LOCUS       DASPGE010000001         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_118626, whole genome shotgun sequence.
ACCESSION   DASPGE010000001 DASPGE010000000
VERSION     DASPGE010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118626"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /locus_tag="VD791_00005"
     CDS             <1..1435
                     /locus_tag="VD791_00005"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV06385.1"
                     /translation="VPIAEQRTQDFRWGLERFSQEARTLARFSHPHIVRVNRYFEANG
                     TGYMVMDYERGDPLKAVFQARPFPPESTIKALIGPLLDGLEKVHAAGFLHRDIKPDNI
                     FLRQDGGAVLIDFGSARQAAGESGSALTTIVTPGYAPFEQYTTSKQQGPWSDIYSLGG
                     VLFFLVTGHSPPDALTRMRGDTLGERLAQARSRYSPALIEAIAWSLAIEEKSRPCDVA
                     TWRKALLGSAAPERNAPPSSIGLETVRVEPRVPTTVLRSDAPGMTTVHLQLADASILL
                     LESGADAAGPRRAAAGRGAPGADWAFGYLLLIVVTAALGAWIGKLPAFQETQMLGGKL
                     NAATLAQLLGAGGAIVFLWLLARSVALRFQAEGGTRAVLYDLAMPIVTLVSQAIAWIV
                     LQKLLGSALEETLSPVYAWGFLLGTCACAMWFSMAVFSHAEPLRKLVHGVRRPRPGPG
                     EPAAGVAGGDSGGGEPGRLTGIGLKER"
     gene            1435..2010
                     /locus_tag="VD791_00010"
     CDS             1435..2010
                     /locus_tag="VD791_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXV06386.1"
                     /translation="MGSWSWIIRYAVVLLVAVLLGTGIAELPVFKQTTLGTPKLPASA
                     LARVLGYGGALVIFWMLGQRTAAELRRAGGGAAQLGFLVVPLATLIVLSAGYDIGLTI
                     LRPFLGAGYKNAYNWLFVLGISASALWLVVALYQRSEGLVDLLKVARAHAPSAARKCE
                     SCGAALADEAKSCTACGKGLAEVDGAHTPAS"
     gene            2112..>2497
                     /locus_tag="VD791_00015"
     CDS             2112..>2497
                     /locus_tag="VD791_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HXV06387.1"
                     /translation="MRVFVTGASGYIGGSVAAGLLAAGHTVKGLVRSEQRAQQVRALG
                     MEPAIGTLSDASLLAQMAREVDAVVNAANSDDRGAVEAMLPALAGSGKAFLHTSGSSI
                     VGDMAAGARGDKMYEDDTPIQPLPGRA"
BASE COUNT          356 a          829 c          915 g          397 t
ORIGIN      
        1 ggtgccgatc gccgagcagc ggacgcagga cttccgctgg ggcctggagc gcttcagtca
       61 ggaggcgcgc acgctcgcga ggttcagcca tccgcacatc gtgcgcgtga accgctattt
      121 cgaggccaac ggcaccggat acatggtgat ggactacgag cgcggcgacc cgctgaaggc
      181 ggtgttccag gcgcgcccgt tcccgcccga gtccacgatc aaggcgctca tcggccccct
      241 gctcgacggt ctggaaaaag tccacgccgc gggattcctg catcgggaca tcaagccgga
      301 caacatcttt ctgcgccagg acggcggcgc ggtgctgatc gactttggct cggcacggca
      361 ggcggccggc gaatccggtt cggccctgac cacgatcgtg acccccggct atgcgccgtt
      421 cgagcagtac acgacctcca agcagcaggg accctggtcc gacatctatt ccctgggcgg
      481 cgtgctgttc ttcctggtga ccgggcacag tccgccggac gcgctcacgc gcatgagggg
      541 cgatacgctc ggcgagcgac tggcccaggc gcgcagccgc tacagccccg cgttgatcga
      601 ggcgatcgcc tggtcgctgg cgatcgagga gaagtcgcgg ccgtgcgacg tggcaacctg
      661 gcgcaaggcg ctgttgggct cggccgcgcc ggagcgcaac gcgccaccct cgtcgatcgg
      721 cctggaaacc gtgcgcgtgg aaccgcgcgt gccgactacg gtgttgcgat ccgatgctcc
      781 gggcatgacc accgtgcact tgcaacttgc ggacgcgtcc atcctgttgc tcgaatcggg
      841 ggctgacgcg gcaggcccgc ggcgcgccgc ggcagggcgc ggcgcgccgg gcgcagactg
      901 ggcgttcggc tatcttctgc tgatcgtggt caccgccgcg ctcggcgcct ggatcggtaa
      961 gctgccggcg ttccaggaga cgcagatgct gggcggcaag ctcaatgccg cgacgctggc
     1021 gcaactgctg ggagccggcg gcgccatcgt cttcctctgg ctgctggccc gaagcgtggc
     1081 gctgcgcttc caggccgagg gcggcacacg cgcggtgctc tacgatctgg cgatgccgat
     1141 cgtcacgctc gtgtcccagg ccatcgcctg gattgtcctg cagaagctgc tcggatctgc
     1201 gctggaggag acgctcagcc cggtctacgc gtggggcttc ctgctcggca cctgcgcgtg
     1261 cgcgatgtgg ttcagcatgg ccgtgttcag tcacgccgag ccgttgcgca agctcgtgca
     1321 cggagttcgc cgcccgcgtc ccggtcccgg cgagcccgct gctggagtcg ccggcggcga
     1381 ctccggcggc ggcgagccgg gccgcttgac gggcatcgga ttgaaggaga ggtgatggga
     1441 tcgtggagct ggatcatccg ctacgcggtg gtgctgctgg tcgccgtgct gctcggcacg
     1501 ggcatcgcgg agctgccggt gttcaagcag accacgctgg ggactccgaa gctcccggct
     1561 tccgcgctgg cccgggtcct gggctacggc ggcgcgctcg tcatcttctg gatgctcgga
     1621 cagcgcaccg ccgccgagtt gcgacgcgcc ggcggtggcg ccgcccagct cgggttcctg
     1681 gtcgttccgc tggcgacgct gatcgtgctg agcgccgggt acgacatcgg gctcacgatc
     1741 ctgcgtccgt tcctcggcgc gggctacaag aacgcctaca actggctgtt cgtgctggga
     1801 atctccgcct cggcgctgtg gctggtggtg gccctgtacc agcgttccga agggctcgtc
     1861 gatctgctga aggtcgcgcg cgcgcacgct ccgtcggcgg cgaggaaatg cgagtcctgc
     1921 ggcgccgcct tggcggacga agcgaagtcc tgcaccgcct gcgggaaggg cctcgccgaa
     1981 gtggacggcg cgcacacacc ggcgtcgtaa cgccgtctag aatctccatg cgcgaactcc
     2041 ccggacgcgg tagtcgccgg ggcgcgggcg atcgtgaggt cccgcgcgcc gatcgacgca
     2101 ggagacatcg catgagagtg ttcgtgaccg gagcctccgg ctatatcggc ggatcggtgg
     2161 ccgcggggct gctcgcggcc ggccacacgg tgaaggggct ggtgcgatcc gagcagcgtg
     2221 cgcagcaggt gcgcgcgctg gggatggaac cggcgatcgg gacgttgagc gacgcctcgt
     2281 tgctggcgca aatggcgcgc gaggtcgatg cggtggtgaa cgccgccaac tcggatgatc
     2341 gcggcgcagt cgaggcgatg ctgccggcgc tcgccggcag cggcaaggcg ttcctccaca
     2401 ccagcggatc gagcatcgtg ggcgacatgg cggccggcgc gcggggcgac aagatgtacg
     2461 aggacgacac gccgatccag ccgctgccgg gaagggc
//
LOCUS       DASPGE010000002         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1172682, whole genome shotgun sequence.
ACCESSION   DASPGE010000002 DASPGE010000000
VERSION     DASPGE010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172682"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..721
                     /locus_tag="VD791_00020"
     CDS             14..721
                     /locus_tag="VD791_00020"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HXV06388.1"
                     /translation="MGYDSAGFTSPRCTLVNRKAGTDVLVVRRARTCAAGVAGCQAAA
                     VGMPYIQTSLCATEAASHRLGLEGATSFDLTKKDCTTPADKRQYLTRIYYISTDNGDG
                     VTLPTLMRLELTGGGWIALPLVEGIEEFNIEYGMDSDGDGAPDVYAANPSDHPKGACA
                     GACPVNNWMNVVTARFHLLARNLDPSPSYSDGKTYELGQDDNGSTVTVTPGGSFRRHV
                     YSALVRLPNAAGRRETP"
     gene            718..1278
                     /locus_tag="VD791_00025"
     CDS             718..1278
                     /locus_tag="VD791_00025"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HXV06389.1"
                     /translation="MTSQRRQRGATLVVALIMLVLTTLFALSSMKTATTSLMVTGNMQ
                     AKTEALNAAQETIETVISTPQFIANPANAVINPCTGPNTLCTDVTGDGAPEYVTQLVP
                     PPYCMSAKPLKNDALNLALAEDLGCAAGQQQQFGVAGAVTGNSLCASSVWEISAHAQG
                     LNSGANVTVRQGIGVRISADEAGSAC"
     gene            1295..>2247
                     /locus_tag="VD791_00030"
     CDS             1295..>2247
                     /locus_tag="VD791_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06390.1"
                     /translation="MRYPRLVLNAAIAAALCLAATQRAVAEDVDLFVGAAATVANPNV
                     LVIIDNSANWNAASQHWVGAGGESPFKQGQSELRALRTVAGELNDKINLGLMLFTGGQ
                     SGNADGGYVRFHMRQMTPTNKAGLQELIGTATCTNGPNSVNGTPNCMFKNFSGAEQAA
                     TADTDYSATLFEAFKYFGGFTSPAHAHDDIGGTPVDAIAGPSATHFGPVRYAGNPDPK
                     TDIAAFSAPFNTYAPPALASAACAKNYIIFIGNGFPNQDLPATVLNNVGGATSQLSMP
                     VLSTSSQTRTSTIGAACGTGSTSTQRVADCTANIPQSLKDA"
BASE COUNT          424 a          800 c          711 g          312 t
ORIGIN      
        1 gccgttccat gtcatgggct acgacagcgc cggcttcact tcgcccagat gcacgctcgt
       61 caatcgcaag gcgggcaccg atgttctcgt cgtgcggcgc gcccgcacct gtgcggcggg
      121 cgtcgcgggc tgccaggccg ccgcggtcgg catgccttac atccagacct cgctgtgcgc
      181 gaccgaggca gcgtctcacc gcctcgggct cgaaggcgcc acgagcttcg atctgacgaa
      241 aaaggactgc acgacccctg ccgacaagcg ccagtacctc acgcgcatct actacatcag
      301 caccgacaac ggggatggcg taacgcttcc cacgctgatg cggctcgagc tgaccggcgg
      361 cggatggatc gcgctgccgc tggttgaggg catcgaggag ttcaatatcg agtacggcat
      421 ggacagcgac ggcgacggcg cgccggacgt gtacgcggca aacccgagcg accaccccaa
      481 gggcgcgtgc gcaggcgcct gcccggtgaa caattggatg aatgtggtga ccgcgcgttt
      541 ccacctgctc gcgcgcaacc tcgatccttc gccgagttat agcgatggca agacctacga
      601 gctcggccag gacgacaacg gcagtaccgt gaccgtgacg cccggcggat cgttccgccg
      661 ccacgtctac agcgcgctgg tgcgcctgcc gaacgccgcc ggccgcaggg agacgccatg
      721 acaagccagc gccgccagcg cggcgcgacc cttgtagtcg cgctgatcat gctggtgctc
      781 accacgctat tcgccttgag ctcgatgaag accgcgacca ccagcctgat ggtcaccggc
      841 aacatgcagg cgaagaccga agcgctgaac gccgcccagg agaccatcga gacggtgatc
      901 agcacgcccc agttcatcgc caaccccgcc aacgcggtga tcaatccgtg caccgggccg
      961 aatacgctct gcaccgacgt gaccggcgac ggcgcgccgg agtacgtgac gcagctagtg
     1021 ccgccgccct actgcatgag cgccaagccg ctcaagaacg atgcgctcaa cctggcgctc
     1081 gccgaggacc tgggctgcgc cgccggccag cagcagcagt tcggcgtggc cggcgctgtc
     1141 accggcaatt ccctgtgcgc cagctcggtc tgggagattt cggcccacgc gcagggactg
     1201 aactcgggcg cgaatgtgac cgtgcggcag ggcatcggcg tgcgcatcag cgccgacgag
     1261 gccggcagcg cctgctagac gagcgaggcc caccatgcgc tatccccgac ttgtcctgaa
     1321 cgctgcgatc gccgccgcgc tttgcctcgc ggccacgcag cgcgccgtcg ccgaggacgt
     1381 cgaccttttc gtgggggcag cggccacggt ggcgaacccg aacgttctgg tcatcatcga
     1441 caacagcgcg aactggaacg cggcgagcca gcactgggta ggcgcgggcg gcgaaagccc
     1501 gttcaagcag gggcaatccg aactgcgcgc gctgcgcacc gtcgccggcg agctcaacga
     1561 caagatcaac ctcgggctga tgctcttcac cggggggcag agcggcaacg ccgatggcgg
     1621 ctacgtacgc ttccacatgc gtcagatgac gccgaccaat aaggcggggt tgcaggagct
     1681 gatcggcacg gcgacttgca ccaacggccc caacagcgtg aacggcacgc cgaactgcat
     1741 gttcaagaac ttcagcggcg ccgagcaggc cgcgaccgcg gacaccgact acagcgcgac
     1801 cctgttcgag gcgttcaaat acttcggcgg ctttaccagc ccggcgcacg cgcacgacga
     1861 tatcggcggc acgccggtgg acgccatcgc gggcccgagc gctacccact tcgggccggt
     1921 gcgctacgcc ggcaaccccg atccgaaaac cgacatcgcg gctttcagcg cgccgttcaa
     1981 tacctatgcg ccgcccgcgc tcgcgagcgc agcctgcgcc aagaactaca tcatcttcat
     2041 cggcaacggt ttcccgaacc aggacctgcc cgccaccgtg ctgaacaatg tcggcggcgc
     2101 cacgagccag ctctcgatgc cggtgctttc cacgagcagc cagacccgca cctcgacaat
     2161 tggagccgcg tgcggcaccg gcagcaccag cacccagcgc gtcgccgatt gcaccgcgaa
     2221 cattccgcag agcctgaagg acgccaa
//
LOCUS       DASPGE010000003        19539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_469937, whole genome shotgun sequence.
ACCESSION   DASPGE010000003 DASPGE010000000
VERSION     DASPGE010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19539
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469937"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..485
                     /locus_tag="VD791_00035"
     CDS             165..485
                     /locus_tag="VD791_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV06391.1"
                     /translation="MTRLLATLAALAIGVAPAMARDYRAGKQKAESCKACHGEAGKEP
                     ITPETPRLAGQHYDYLVHALQAYKKGTRDNALMSPMARPLSEADIRDMAWYFSRQKGL
                     QTKY"
     gene            complement(584..1237)
                     /gene="nth"
                     /locus_tag="VD791_00040"
     CDS             complement(584..1237)
                     /gene="nth"
                     /locus_tag="VD791_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXV06392.1"
                     /translation="MNPAKREEIFRRLQARNPTPTTELEYRTPFELLVAVVLSAQATD
                     KSVNIACRRLFPVAATPQALLALGEDGLAQHIRSIGLYRTKAKNLMAACRLLIERHGG
                     AVPHERAALESLPGVGRKTANVILNTAFGEPTIAVDTHIFRVANRTGLAPGKTVEEVE
                     RKLLKFVPERYRKDAHHWLILHGRYVCKARRPECPACPILDLCEYRAKTKASNDIRS"
     gene            complement(1234..1896)
                     /locus_tag="VD791_00045"
     CDS             complement(1234..1896)
                     /locus_tag="VD791_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HXV06393.1"
                     /translation="MRSSPATADEIDALLPQTQCTKCGFGGCRPYAEALAAGQAQINR
                     CPPGGAAGVVKLAALLGRPAPPLDPDCGREGPRMAALIDEARCIGCTLCLRACPTDAI
                     VGAPKQMHTVLLAQCTGCELCLPLCPVDCIDMLPLVQLAQRGSAVARREARTPVAAHA
                     ARWRERYRRHQDRLARECEQRELGLAAAEQDRRAGPDAERKRAAVRAALERARARAAG
                     NP"
     gene            complement(1903..5427)
                     /locus_tag="VD791_00050"
     CDS             complement(1903..5427)
                     /locus_tag="VD791_00050"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXV06394.1"
                     /translation="MTPVSTASPLRLAHGLQFEALYQRDGLLRLDAAFIAQLGQAAPA
                     LADALQAARADPDACTPLQESELLIALAPYLDDFVAELFGIEVEVRALSARHHALAPL
                     YAVKRGFVQRRAMARIKPQEAAAVDGPALEAELAAAMGGAFTELGFAQCVSAWLEDEA
                     GCAAQLERAARYAAWAAHTPAGRARHRTGVLFKAPAKLDYFRLVPAQAEDAGGHRSFR
                     SAHLRRREGFALTDPGTDLTGALDHANYCIWCHEQGRDSCSRGLKEKAAGDAPPAFRK
                     TPFGVPLAGCPLEEKISEFHKVKVLGHAIAALAMIAVDNPMAAATGHRICNDCMKSCI
                     YQKQEPVNIPQAETRTLKDVLELPWGFEIYGLLTRWNPLDLRRPYPRPRSGRRVLIVG
                     LGPAGFTLAHHLINDGHLVVGIDGLKIEPLPPELSGVDERGARVPFAPLRDIQALYET
                     LDERVMAGFGGVAEYGITVRWDKNFLKVIRLLLERRAQFSMFGGVRFGGTLSAEDAWA
                     QGFDHIALAAGAGRPTVLELPNGLARGVRTASDFLMALQLTGAAKTDSVANLQIRLPV
                     VVIGGGLTAIDTATESAAYYPVQVEKFLLRYETLAAERGKAVVRAGWSAEEHAIADEF
                     IAHARALRAERAVAAAEGRPPRVAELLQSWGGVTIAYRRRMIDSPSYTLNHEEIEKAL
                     EEGIRFAEGLTPVAVEVDAFGHASGLRVAAQKADEHGTLHPTTETVLPARAILVAAGT
                     QPNTVLAREDAAHFPLDGRYFQACDEDGKPVAPERSAKPRAVRVLLAREPDGRFISFF
                     GDLHPSFFGNVVKAMGGAKQGYPVVSRVLNARAPAWPGSDEAFVWQLNDALRASVHEV
                     LRLTPDIVEVVVRAPAAARRFRPGQFYRLQNYERFAAQVDGTRLAMEGLAMTGAWVDR
                     ARGLVSTIVLEMGGSSDLCAWLRPGEPVVLMGPTGSPTDIPSGETVLLVGGGLGNAVL
                     FSIGQALRQAGSRVLYFAGYKKMIDRYKVAEIEAASDLVVWCCDEAPGFVPTRPQDRA
                     FVGNIVQGLLAYAEGRLGEPGVPLKEAQRLIAIGSDRMMAAVAAARHGVLAAHLDPRH
                     VAIGSINSPMQCMMKEICAQCLQAHVDPATGRSTYVFSCFNQDQPLDRVDFAGLGARL
                     AQNALQEKLTAQWIDRCLVRLARRAAAA"
     gene            complement(5618..6646)
                     /locus_tag="VD791_00055"
     CDS             complement(5618..6646)
                     /locus_tag="VD791_00055"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011884670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HXV06395.1"
                     /translation="MSYRWLRPLLFRLEAEAAHAATLRLLELGAALRLPLAPIPPPAP
                     VRVMGLEFPNPVGLAAGLDKNGAHIDALARLGFGFLEVGTVTPRPQPGNPRPRQFRIP
                     EARAVINRMGFNNLGVDRLVHNVRAARYRGILGINIGKNFDTPIERADADYLACMRKV
                     YALASYVAINISSPNTADLRRLQEADALDRLLPQLKTEQARLADQHGRYVPIAVKIAP
                     DFAPEQVRSLAERLQAHAVDAVIATNTTLSREGVEGLPQGRESGGLSGAPLTARALGI
                     VRLLAQHLQERIPIIGVGGIMSGADAAERIAAGASLVQLYTGLVYAGPALICDCVRAL
                     RAIRPAAA"
     gene            complement(6643..7428)
                     /locus_tag="VD791_00060"
     CDS             complement(6643..7428)
                     /locus_tag="VD791_00060"
                     /inference="COORDINATES: protein motif:HMM:NF005126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HXV06396.1"
                     /translation="MSLERITVETRGGVRLLGLNRTAKRNAFDLQMYAELAQAYGDYE
                     RDAKARCALLFAHGEHFTGGIELPQWLPLFRDGRMPPLPAGAIDPLLRTQALSKPLVI
                     AVQGWCLTIGIELLLAADIRVAAQGTRFAQIEVKRGIFPIGGATVRLVQEAGWGNAMR
                     ILLTGDEFSAAEAFRIGLVQEVAAPGAQLERALVIAQTIARQSPTGVRATLASARLAR
                     EQGERAAFARMLADMQTAVAGKDAIEGLNAFLERREPRFDDPT"
     gene            complement(7580..8314)
                     /locus_tag="VD791_00065"
     CDS             complement(7580..8314)
                     /locus_tag="VD791_00065"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HXV06397.1"
                     /translation="MTQLNDLPFSVLQFYATAPYPCSYLPDRLARSQVATPSHAIDPQ
                     VYSQLVRAGFRRSGAFTYRPYCDSCRACVPVRILAREFIPNRSQRRAAGRHAGLDARV
                     LDLKYNPEHYALYLRYQSTRHAGGGMDHDSREQYRHFLLQSNVNSSLVEFREDGRLRM
                     VSIIDRLEDGLSSVYTFFDPDLSGGSLGTYNVLWQVDLCRRLNLPYLYLGYWIAQSRK
                     MAYKVNFQPVEGLVNGQWQRLPQQRA"
     gene            complement(8328..9032)
                     /gene="aat"
                     /locus_tag="VD791_00070"
     CDS             complement(8328..9032)
                     /gene="aat"
                     /locus_tag="VD791_00070"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HXV06398.1"
                     /translation="MIPWLSEHDDFPPVERARRRPNGLLAAGGDLSPQRLLRAYRRGI
                     FPWYSAGEPILWWSPDPRMVLLPGELHVGRSLRKTLRKAPFVVTADTAFERVLEGCSA
                     PRPDQDGTWITPDMKRAYGALHALGLAHSVESWAGDELVGGLYGVALGRVFFGESMFS
                     RAGDASKVAFVHLVRQLSAWEFGLIDCQMRTPLLAAFGAREIPRREFTRRLAELVNYP
                     DVAGRWSLPAPAHDRS"
     gene            complement(9052..9585)
                     /locus_tag="VD791_00075"
     CDS             complement(9052..9585)
                     /locus_tag="VD791_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HXV06399.1"
                     /translation="MTPRTVLAAVAVATLAACANMSLRSPTAVANLAPTKGNQVAGTV
                     NFTQQGELVLVEAHVTGLTPGAHGFHIHEKGNCTAPDASSAGGHFNPTGAAHGAPSAS
                     ARHAGDLGNLVADAAGVADYRAEIHGISLGTGEDSIIGRAVIVHAERDDLTSQPTGNA
                     GARQACGLISLSKDKWF"
     gene            9838..11400
                     /gene="fliF"
                     /locus_tag="VD791_00080"
     CDS             9838..11400
                     /gene="fliF"
                     /locus_tag="VD791_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HXV06400.1"
                     /translation="MAQAPGTGIAALTRGFTQLSNSQKLGFLAALAAVAAVIVVALLW
                     AGAPEYRVLYSNLSERDGGEVIGALAQLDVPYRVGEGGSVVLVPAGQVYELRLKLAAQ
                     GLPKGGAVGFELMDGQKFGLSQFAEQVNYQRALAGELARSVQSMVAVQSARVHLAIPR
                     PTVFVREARQPSASVFVATYPGRALDTGQVAAIQHLVASSVPELPARNVTVVDQGGNL
                     LSRGGAEAAGNALDASQLQYLHTVESSYARRIEAILEPIVGRDNVRAQVTAALDFSRS
                     EQTSETFKPNAASAATIRSQQTTMQTVPASGAQPGQAAAARAAAPGTRRESVVSYEVD
                     KTVRHTKGEVGAVKRLSAAVVVNYRREAGDGGAYQPLGEETLQQITALTREAMGFDPD
                     RGDTLNLVNAPFNGEVAAGAAGESTLAAPTGWIQDIASPAGMTLLKTALVALVLLAAL
                     WLARSVLRELARAGRRSPGRALPGSGAEAQLAAAPPEGAGYEADLRAVRDMARQDPRT
                     VANVVREWVARQ"
     gene            11397..12389
                     /gene="fliG"
                     /locus_tag="VD791_00085"
     CDS             11397..12389
                     /gene="fliG"
                     /locus_tag="VD791_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HXV06401.1"
                     /translation="MRDGVTRSAILLMSLGEEEAVEVFKLLAPREVQKVGQAMARLGR
                     VSRDDVAGVLRDFRQVAEQQVPIGTASDQYLRSVLTRALGDDRAAGLIDRILQVQDNR
                     GIEGLKWMDAASVAELIRNEHPQVIATILAHLDADQAAGVLGALDEQLRGDVVLRVAT
                     LESVQPAAMQDLNEVLAQLLSGGERAGKSALDGVQAAAQILNRMGPMEAAVCSHIREV
                     DEELAQRIEDRMLAFDDLLELDDRDLQLLLREVPSETLLVALKGAGAALQDKVFRNLS
                     QRAAEMLREDLEAKGPVRLSQVEAEQREILRIARRLAADGLVVLGGAGGASVVG"
     gene            12379..13020
                     /locus_tag="VD791_00090"
     CDS             12379..13020
                     /locus_tag="VD791_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HXV06402.1"
                     /translation="MSGRIIPREQAQRARKLDLGDIAAGRSPIDTRRAGGEQDASPAD
                     IERVREQAYREGVEAGRREAGAQLARQRDELRALIQGVNELLENVEQRLATEVLSISL
                     ELAKLIVRAGVRVRPELVLSVVQDAIASLPGAGEPVTLLLNPADAALLRELARDDRAL
                     SALPWKLLEDPHIERGGCKLETPSTEVDGTLETRWRRVIANLGRDDAWIDITT"
     gene            13034..14431
                     /gene="fliI"
                     /locus_tag="VD791_00095"
     CDS             13034..14431
                     /gene="fliI"
                     /locus_tag="VD791_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HXV06403.1"
                     /translation="MSRVRRWQKYVEDCRTAVRAAQPFTVSGRLTRVAGLVMEAVGLK
                     LPVGNSCYVVTPSGQRVDAEVVGFSGERLFLMPSTDVYGLKPGALVVPIDQAVMRVPR
                     LGEDYVPRRRAQDRAKHVPVGAGLLGRVVDGAGRPLDNAGPLALDRHVPLHSRPINPM
                     ERAPIRTVLDVGIRAMNALLTVGRGQRLGLLSGSGVGKSVLLSMMARYTAADVVVVGL
                     IGERGREVREFIETILGREGLERSAVVAAPADSPPLLRLHGAAYATAIAEHFRDQGRH
                     VLLIMDSLTRYATAQREISLAIGEPPATRGYTPSVFAKLPQLIERAGNGPDNRGSITA
                     FYTVLTEGDDLQDPIADAARAILDGHIVLSRDLADQGHYPAIDIESSISRAMTELLSA
                     EELALARRFKQLFAAYRRSRDLIAVGAYVRGNDPLLDRAVALYPQLAAFLQQDMYERA
                     SVAASRERLRSLLEE"
     gene            14431..14907
                     /gene="fliJ"
                     /locus_tag="VD791_00100"
     CDS             14431..14907
                     /gene="fliJ"
                     /locus_tag="VD791_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HXV06404.1"
                     /translation="MAAAFALQPLLDLAERRLEAATAELQRLAALRQQAEGSLAQLRG
                     RHAQYAAELQRALARGIEPDRLRDYRAFLARLERAVALQAEELERRRRAWADAHARWL
                     ELRRREQALAVLRRRHQLAEAVREGRREQRQQEELISRKSGHEALEDVPLEGALRR"
     gene            15022..16041
                     /gene="fliM"
                     /locus_tag="VD791_00105"
     CDS             15022..16041
                     /gene="fliM"
                     /locus_tag="VD791_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HXV06405.1"
                     /translation="MADDTLSQEEVEALLRGVKGAPGQVPAPAAMDSVRSYDIGHRRR
                     IVRGGMPGLELVNQRFANLLQASLSRLTRRSAEVTAGPPQVLEYSELVRNLTTPASLS
                     LVQAKPLRGVGLVVLDADLVSQTIDQLFGGQGRFAEPAQGAHGREFTATELRVARRLL
                     DAVLEAYGKAWEPVFPLRFEHLRSETDARFANIAAPDELVVTSSFSVDAGLVDANPVD
                     ANRGGARFHVCLPYAILEPIRGIVDQPMQAERSDANDRWTALLSAQVPDIEVELAANL
                     ARTAISVRQLMRLESGDVIAIELPEVIAAEVDGVPVLECKYGVVHGRYALKVVRRAQD
                     RGSGS"
     gene            16060..16470
                     /gene="fliN"
                     /locus_tag="VD791_00110"
     CDS             16060..16470
                     /gene="fliN"
                     /locus_tag="VD791_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HXV06406.1"
                     /translation="MADDDVKAGVDAWAPLGSQAGSQSVSSSAAGSGARVFPELTTEP
                     GARGAAASLDIVMDIPVQLSVELGRTRLPIRTLVQLAQGSVVELDRLAGEPLDVLVNG
                     CLIAQGEVVVVNDKLGIRLTEVIGPDERLRRLQR"
     gene            16493..16927
                     /gene="fliO"
                     /locus_tag="VD791_00115"
     CDS             16493..16927
                     /gene="fliO"
                     /locus_tag="VD791_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HXV06407.1"
                     /translation="MRPPVARWVAGLAAGVSLALPVAGADAPASGTAVPVGGLLQVVF
                     ALLLVLGAIGLFAWLMRRFVPGHGTAGGVLKVVGGVMVGPRERLVVVEVGDTWLFVGV
                     AASSVRLVHSMPRPERSAAAPAQATSFTRRLRHALALRRGEG"
     gene            16935..17750
                     /gene="fliP"
                     /locus_tag="VD791_00120"
     CDS             16935..17750
                     /gene="fliP"
                     /locus_tag="VD791_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024001.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HXV06408.1"
                     /translation="MRNHPASTGAPLSGTTGWRRPGGGAQRWLGVVLLLAAAAAAREA
                     GAQALPAITSAPAPGGGQTYSLTVHTLLFLTSLAFLPAVVLTMTAFTRIIIVLALLRQ
                     ALGTVQAPPNQVLVGLALFLTFFVMAPVFGRMYDEAYVPLSERRIGTEQALERAAVPL
                     RGFMLRQTRHADLALFAQLAGEPPPADADAVPMRVLLPAYVISELKTAFQIGFVVFIP
                     FLIIDLVVASVLMSMGMMMLSPVIVALPFKLMLFVLVDGWNLLVGSLLQSFSA"
     gene            17747..18016
                     /gene="fliQ"
                     /locus_tag="VD791_00125"
     CDS             17747..18016
                     /gene="fliQ"
                     /locus_tag="VD791_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HXV06409.1"
                     /translation="MTPELVVDLVQQALRVGILVAAPMLLAALLAGLIVSVFQAATQI
                     NEMTLPFVPKLLALFAAAVLAGPWMLGLMVDFTRRLFSSIPGVVG"
     gene            18023..18835
                     /gene="fliR"
                     /locus_tag="VD791_00130"
     CDS             18023..18835
                     /gene="fliR"
                     /locus_tag="VD791_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HXV06410.1"
                     /translation="MVQISAGMIQISSGQWYGWIAAFLYPLLRVLGLILADPLLGNRS
                     VPITAKLGLALLIAVLVAPLLPPAPSADPASAAGVLIAAQQLLIGLAMGFSIRVALSA
                     AQMAGQLAGLQMGLTFAQFFDPHSGAQSAVLDRFLGLFAVLIFFSIDGHHVVLGVLVD
                     SFRALPIAAAPVDALGLRVVVEWAGTIFTAGLLIALPVVATLLIANAALGIVMRAAPP
                     LNLFTVGFPVTLITGLLALYLVAPYLGPALADLFEQATLALGRVLEALTPGS"
     gene            18950..>19539
                     /gene="dapA"
                     /locus_tag="VD791_00135"
     CDS             18950..>19539
                     /gene="dapA"
                     /locus_tag="VD791_00135"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HXV06411.1"
                     /translation="MLKGSLVAIVSPMHEDGSLDLESFRGLVDWHIAQGTDGIVVVGT
                     TGESPTVDFEEHCLLIRTAVERAGGRVPVIAGTGANSTREAIELQAFAHKAGVDMTLS
                     VVPYYNKPTQEGLYRHFKAIAESVDLPLIMYNVPGRTVCDMHNDTALRLAQVPNIVGI
                     KDATGNLERGADLVMRAPKDFAVYSGDDATAIALMLL"
BASE COUNT         2801 a         6812 c         7044 g         2882 t
ORIGIN      
        1 ggccagacgc agagctacct ggtcaaggcg ctgcaggatt accgctccgg cgtgcgccag
       61 caagcatcga tgaacggtgt ggccgccacg ctgagcgatc aggacatcgc cgacctggcg
      121 gcctattacg ccggcgacgc caggtgaacg tggcgatgag cacgatgacc aggcttctgg
      181 cgacgctcgc cgcactggcg atcggcgttg cgccggccat ggcgcgcgac taccgggccg
      241 gcaagcagaa ggcggaaagc tgcaaggcct gccacggcga ggccggcaag gagccgatca
      301 cgcccgaaac gccgcgcctc gcagggcagc actacgatta cctggtgcac gcgctgcagg
      361 cctacaagaa gggcacgcgc gacaacgccc tgatgagccc gatggcgcgg ccgctcagcg
      421 aggccgacat ccgcgacatg gcgtggtact tctccaggca gaaggggctg cagaccaagt
      481 actagccggc gcctgaatgc acccttaagg cggcgcgatt cgaacagccc agaacccagg
      541 ttccggcgtc gcactgacga ctgggggtga acatcgccgg gggtcaggag cggatgtcgt
      601 ttgacgcctt ggtcttggcg cggtactcgc acaggtccag gatcgggcag gccgggcact
      661 ccggccgccg cgccttgcac acgtagcgcc cgtgcaggat cagccagtgg tgcgcatcct
      721 tgcgatagcg ctcgggcacg aacttcagca gcttgcgctc gacttcctcc accgtcttcc
      781 cgggagccag cccggtgcgg ttggccaccc ggaagatgtg cgtgtccacg gcgatcgtgg
      841 gttcgccgaa ggcggtgttg aggatcacgt tcgcggtctt gcgccccacc cccggcaggc
      901 tttccagcgc cgcgcgctcg tgcgggaccg cgccgccgtg acgctcgatc agcaggcggc
      961 aggcggccat caggttcttc gccttggtgc ggtacagccc gatgctgcgg atgtgctgcg
     1021 cgaggccgtc ctcgcccagg gcgagcaacg cctgcggcgt ggcggcgacc gggaacaagc
     1081 ggcggcaggc gatgttcacg ctcttgtccg tggcctgcgc ggacagcacc acggccacca
     1141 gcagctcgaa cggcgtgcgg tactccagct cggtggtggg cgtcgggttg cgcgcctgca
     1201 gccggcggaa gatctcctcg cgcttggccg gattcatggg ttgccggcgg cccgcgcccg
     1261 cgcccgttcg agcgccgccc ggaccgcggc gcgcttgcgc tccgcatcgg gccccgcgcg
     1321 ccggtcctgc tccgcagctg cgagcccgag ctcgcgctgt tcgcactcgc gggcgagcct
     1381 gtcctggtgc cggcgatagc gctcgcgcca gcgcgcggcg tgcgcggcaa ccggcgtgcg
     1441 ggcctcgcgt cgggccacgg ccgacccgcg ctgcgccagc tgcaccagcg gcagcatgtc
     1501 gatgcagtcc accgggcaga gcggcaggca cagctcgcag ccggtgcact gcgcgaggag
     1561 cacggtgtgc atctgcttgg gggcgccgac gatcgcgtcg gtggggcaag cgcggaggca
     1621 gagcgtgcag ccgatgcaac gcgcctcgtc gatcaacgca gccatgcgcg gcccttcccg
     1681 cccgcagtcc ggatcgagcg gcggcgcggg tcgcccgagc aaggcagcca gcttgaccac
     1741 gcccgccgcg ccgcccggcg gacagcggtt gatctgcgcc tgtcccgccg cgagcgcctc
     1801 cgcgtagggc cggcagccgc cgaagccgca cttcgtgcac tgcgtctgcg gcagcagcgc
     1861 gtcgatttcg tctgcagtgg ccggcgacga tcgcattcgg gctcaagccg cggcggcgcg
     1921 gcgtgcgaga cgcaccaggc agcggtcgat ccactgggcg gtgagcttct cctgcagcgc
     1981 gttctgcgcg agccgcgcgc ccaggccggc gaagtcgacc cggtccagcg gctggtcctg
     2041 gttgaagcag gagaacacgt aagtgctgcg gccggtggcc gggtccacgt gcgcctgcag
     2101 gcactgcgcg cagatctcct tcatcatgca ctgcatgggt gagttgatcg agccgatcgc
     2161 cacgtggcgc ggatcgaggt gcgcggccag cacgccgtgg cgggcggcgg ccaccgccgc
     2221 catcatgcgg tccgagccga tcgcgatcag gcgctgcgct tccttcagcg gcacgccggg
     2281 ctcgcccagc cgaccctcgg cataggcgag cagcccctgc acgatgttgc cgacgaaggc
     2341 gcgatcctgc ggccgcgtgg gaacgaaacc gggcgcctcg tcgcagcacc agaccacgag
     2401 gtcgctggcg gcctcgatct ccgccacctt gtagcgatcg atcatcttct tgtagccggc
     2461 gaagtacagc acgcgcgacc ctgcttggcg cagcgcctgc ccgatcgaga acagcaccgc
     2521 gttgcccagg ccgccgccga ccaacagcac ggtttcgccc gacgggatgt cggtgggcga
     2581 gccggtgggt cccatcagca ccaccggctc gccggggcgc agccaggcgc acaggtcgga
     2641 ggagccgccc atctccagca cgatggtgga caccaggccg cgggcgcgat cgacccaggc
     2701 cccggtcatc gccaggcctt ccatcgccag gcgcgtcccg tccacctgcg cggcgaagcg
     2761 ctcgtagttc tgcaggcggt agaactgccc gggccggaag cggcgggcgg cggccggcgc
     2821 gcgcaccacc acctcgacga tgtcgggcgt cagccgcagc acctcgtgca cgctggcgcg
     2881 cagggcgtcg ttgagctgcc agacgaaggc ctcgtcgctg cccggccagg ccggcgcccg
     2941 cgcgttcagc acgcggctca ccaccgggta gccctgcttg gcgccgccca tggccttgac
     3001 cacgttgccg aagaacgaag ggtgcaggtc gccgaagaag ctgatgaagc ggccgtccgg
     3061 ctcgcgcgcg agcagcacgc gcaccgcgcg tggtttggcg ctgcgttcgg gcgcgaccgg
     3121 cttgccgtcc tcgtcgcagg cctggaagta gcgcccgtcc agcggaaagt gcgcggcatc
     3181 ctcgcgcgcc agcacggtgt tgggctgggt gcccgcggcc accaggatgg cgcgcgccgg
     3241 cagcaccgtc tcggtggtcg ggtgcagcgt gccgtgctcg tccgccttct gcgccgcgac
     3301 gcgcaacccc gaggcgtgac cgaacgcgtc cacttccacc gcaacgggag tgaggccttc
     3361 ggcgaagcgg atgccttcct cgagcgcctt ctcgatctcc tcgtggttga gggtgtagga
     3421 cgggctgtcg atcatgcgcc tgcggtaggc gatagtgacg ccgccccagg actgcagcaa
     3481 ctcggcgacg cgtggcggcc ggccctcggc ggcggccacc gcccgctcgg cgcgcagcgc
     3541 tcgcgcgtgg gcgatgaact cgtcggcgat ggcatgctcc tccgcactcc agccggcgcg
     3601 caccaccgcc ttgccacgct cggccgcgag cgtctcgtag cgcagcagga acttctctac
     3661 ctgcaccggg tagtaggcag ccgattcggt cgcggtgtcg atggcggtga ggccgccgcc
     3721 gatcaccacc accggcagcc ggatctgcag gttggcgacc gagtcggtct tggccgcacc
     3781 ggtgagctgc agggccatca ggaagtcgga ggcggtgcgc acgccgcgcg cgagaccgtt
     3841 gggcagctcc agcaccgtgg ggcggccggc gccggcggcg agcgcgatat ggtcgaagcc
     3901 ctgcgcccag gcgtcctcgg cgctcaaggt gccaccgaag cgcacgccgc cgaacatgga
     3961 aaactgcgcg cgccgctcga gcagcagccg gatcaccttg aggaagttct tgtcccagcg
     4021 caccgtgatg ccgtactcgg ccacgccgcc gaagccggcc atgacgcgct cgtcgagcgt
     4081 ctcgtacagc gcctggatgt cgcgcagcgg cgcgaacggc acgcgcgcgc cgcgttcgtc
     4141 cacgccggac agctccggcg gcagcggctc gatcttcagc ccgtcgatgc cgaccaccag
     4201 gtggccgtcg ttgatcagat gatgcgcgag cgtgaagccg gccggaccca ggcccacgat
     4261 cagcacgcgc cggccgctgc gcgggcgcgg atacggccgg cgcaggtcga gcgggttcca
     4321 gcgcgtgagc aggccgtaga tctcgaatcc ccagggcagc tcgagcacgt ccttcagcgt
     4381 gcgcgtctcg gcctgcggaa tgttgaccgg ttcctgcttc tggtagatgc aagacttcat
     4441 gcagtcgttg cagatgcgat ggccggtggc cgccgccatc gggttgtcca ccgcgatcat
     4501 cgccagcgcg gcgatcgcgt gaccgagcac cttgaccttg tggaactcgg agatcttctc
     4561 ctccaggggg cagccggcaa gcggcacgcc gaacggcgtc ttcctgaagg cgggcggggc
     4621 gtcgccggct gctttttcct tgaggccgcg ggagcacgag tcgcgtccct gctcgtggca
     4681 ccagatgcag tagttcgcat ggtccagggc accggtcagg tcggtgccgg gatcggtgag
     4741 ggcgaaaccc tcacgccggc gcaggtgcgc gctgcggaag ctgcgatggc cgcccgcatc
     4801 ctcggcctgg gccggcacca ggcgaaagta gtcgagcttg gcgggcgcct tgaacagcac
     4861 gccggtgcga tggcgcgcgc ggcctgcggg agtgtgcgcc gcccacgcgg cgtagcgcgc
     4921 cgcgcgctcg agctgcgcag cgcaaccggc ctcatcctcg agccaggcgc tcacgcactg
     4981 cgcaaagcct agctcggtga acgccccgcc catggcggcc gcgagctcgg cttcgagcgc
     5041 cgggccgtcc accgcggccg cctcctgcgg cttgatgcgc gccatcgccc ggcgctgcac
     5101 gaaaccgcgc ttcaccgcgt acagcggcgc cagcgcgtgg tgccgcgcgc tcagcgcgcg
     5161 cacttcgacc tcgatgccga acagctcggc gacgaagtcg tcgagatagg gcgccagcgc
     5221 gatcagcagc tccgactcct gcagcggggt gcaggcatcc ggatcggccc gcgccgcctg
     5281 caaggcgtcc gccagcgccg gcgcggcctg cccgagctga gcgatgaacg cggcgtcgag
     5341 gcgcaacagc ccgtcgcgct gatacaaggc ttcgaactgg aggccgtgcg cgagccgcag
     5401 cggagaagcg gtggaaacgg gcgtcatcga gggcaacaca ggaaaactac aaagtatagc
     5461 agccgacccg cgtcgctccg cgcgcaggca accacgcgct gcgtagggcc gcacccgtcc
     5521 cagcgtcggc accaccgttc cagcgtcggc acctcccggc gtccgcacct cctggcgtcg
     5581 gcgctcggcc gtgcgctgca cccgccttgc gccctggcta tgccgcggcc gggcggatcg
     5641 cgcgcagcgc ccgcacgcaa tcgcagatca gcgcgggccc cgcgtagacc aggccggtgt
     5701 agagctgcac caggctggcc cccgcagcga tgcgctcggc cgcgtccgcg ccgctcatga
     5761 tcccgccgac gccgatgatc gggatgcgct cctgcaggtg ttgggccagc agccgcacga
     5821 tgccgagcgc acgcgcggtg agcggcgcgc cgctcaggcc cccgctttca cggccctggg
     5881 gcagcccctc gacgccttct cgcgacagcg tcgtgttggt ggcgatgacc gcatccacgg
     5941 catgcgcctg caggcgctcc gctagggagc gcacctgctc cggcgcgaaa tcgggtgcga
     6001 tcttgactgc gatcgggacg tagcgcccgt gctggtcggc aagccgtgcc tgctcggtct
     6061 tcagctgcgg caacagccga tcgagggcgt cggcctcctg gagccggcgc aggtcggcgg
     6121 tgttgggcga ggagatgttg atcgcgacat agctcgccag cgcatagacc ttgcgcatgc
     6181 aggcgagata atccgcgtcg gcgcgctcga tcggcgtgtc gaagttcttg ccgatgttga
     6241 tgccgaggat gccgcggtag cgcgctgcgc gcacgttgtg caccagacgg tccacgccca
     6301 ggttgttgaa ccccatgcgg ttgatcaccg cgcgcgcctc gggaatgcgg aattgacgcg
     6361 gacgcggatt gcccggctgc gggcgcgggg tcacggtgcc cacttcgagg aaaccgaagc
     6421 ccaggcgcgc cagcgcgtcg atgtgcgcac cgttcttgtc caggcccgcg gccaggccca
     6481 ccggattggg gaactcgagc cccatcacac gcaccggagc cggcggcggg atcggcgcga
     6541 gcggcaggcg cagcgccgcg cctagctcga gcagccgcag cgtcgcagcg tgcgccgcct
     6601 ccgcctccag ccggaacagc agcggccgta gccagcggta gctcacgtcg gatcgtcgaa
     6661 gcgcggctcg cgccgctcca ggaaggcgtt gagcccctcg atggcgtcct tgcccgcgac
     6721 cgcggtctgc atgtcggcca gcatgcgcgc gaacgccgcg cgctcgccct gctcgcgcgc
     6781 cagccgcgcg gaggcgagcg tggcgcgcac gccggtcggc gattggcggg cgatggtctg
     6841 ggcgatgacg agcgcgcgct cgagctgcgc gcccggcgcc gccacctcct gcaccaggcc
     6901 gatgcggaac gcttccgcgg cggagaactc gtcgccggtg agcaggatgc gcatcgcgtt
     6961 gccccagccc gcctcctgca ccaggcgcac cgtggcgccg ccgatcggga agatgcccct
     7021 cttgacctcg atctgggcga agcgcgtgcc ctgcgccgcc acgcggatgt cagcggccaa
     7081 cagcagctcg atgccgatgg tcaggcacca gccctgcacc gcgatcacca gcggcttgct
     7141 gagcgcctgc gtgcgcagca gcgggtcgat cgcacccgcg ggcaatggcg gcatgcgccc
     7201 gtcgcggaac aacggcagcc actggggcag ctcgatgccg ccggtgaagt gctcgccgtg
     7261 tgcgaacagc agggcgcagc gcgccttggc gtcgcgctcg tagtcgccgt acgcctgcgc
     7321 cagttcggcg tacatctgca ggtcgaaagc gttgcgcttg gccgtgcggt tcagtccgag
     7381 cagccgcacg ccgccgcggg tttccaccgt gatccgctcg aggctcatga ttcagagaac
     7441 gctgtcacca cgaaggacac gaagaagaac ccaagagcac gaacacccga ccaggccttt
     7501 tcttgttgct cggtcttcgt gtccttcgcg ctcttcgtgt gcttggtgtc gcgggtgttc
     7561 ctggtggtga agacgaggtt caggcccgct gctggggcag ccgctgccac tggccgttca
     7621 ccagcccctc caccggctgg aagttgacct tgtaggccat cttgcgggac tgtgcgatcc
     7681 agtagccgag gtagagatag ggcaggttca ggcgccggca cagatccact tgccacagta
     7741 cgttgtaggt gccgaggctg ccccccgaca ggtccgggtc gaagaaagtg tagaccgagg
     7801 acaggccgtc ctccagccga tcgatgatgc tcaccatgcg cagccgtccg tcctcgcgga
     7861 actcgaccag gctggagttg acgttgcttt gcaggaggaa atgacggtac tgctcgcggc
     7921 tgtcgtgatc catgccgccg ccggcgtggc gggtggactg gtagcgcagg tacagcgcgt
     7981 agtgctcggg attgtacttg aggtcgagca cgcgcgcgtc caggcccgcg tgccgtcccg
     8041 ccgcgcggcg ctgcgagcgg ttggggatga actcgcgcgc caggatgcgc accggcacgc
     8101 aggcacggca gctgtcgcaa tacggacggt aggtgaacgc gccgctgcgc cggaagccgg
     8161 cgcgcaccag ctggctgtag acctgcgggt cgatcgcgtg gctgggggtg gcgacctggg
     8221 agcgcgccag ccggtccggc aggtagctgc aggggtaggg cgcagtcgcg tagaactgca
     8281 gcaccgagaa cggcagatcg ttgagctgag tcatgccggg ccgagggtca tgatcgatcg
     8341 tgcgcgggcg ccggcagaga ccagcggccc gccacgtccg gatagtttac caactccgcc
     8401 agccggcgcg tgaactcgcg tcgcgggatc tcgcgggcgc cgaaggcggc gagcaggggc
     8461 gtgcgcatct ggcagtcgat caggccgaac tcccaggcgc tgagctggcg caccaggtgc
     8521 acgaacgcga ccttggaggc gtcgccggcg cgcgagaaca tcgattcgcc gaagaacacc
     8581 cgccccaggg ccacgccgta caggccgccg accagctcat ccccggccca gctctccacc
     8641 gagtgcgcca gccccagcgc gtgcagcgcc ccgtaggcgc gtttcatgtc cggcgtgatc
     8701 caggtaccgt cctggtccgg ccgcggcgcg ctgcagcctt cgagcacgcg ctcgaaagcg
     8761 gtgtcggcgg tgaccacgaa cggcgccttg cgcagggtct tgcgcagcga gcggccgacg
     8821 tgcagttcgc cgggcaacag caccatgcgc gggtccgggc tccaccacag gatcggctcg
     8881 ccggcgctgt accagggaaa gatgccgcgc cggtacgcgc gcagcagccg ctgcggcgac
     8941 aggtcgccgc cggcggcgag caatccgttg ggtcgccgcc gcgcacgctc caccggcgga
     9001 aagtcgtcgt gctcggaaag ccagggaatc aatcgttgcg gcggggagcg ctcagaacca
     9061 cttgtccttg ctcaagctga tcagcccgca ggcctggcga gcgccggcgt tgccggtggg
     9121 ctggctggtg agatcatcgc gctcggcgtg cacgatgacg gcgcggccga tgatgctgtc
     9181 ttcgcccgtg cccaggctga tgccgtggat ctcggcgcga tagtcggcca cgccggcggc
     9241 gtcggccacc aggttgccga ggtccccggc gtgacgtgcg ctggcgctcg gcgcgccatg
     9301 cgcggcgccg gtgggattga aatgcccgcc ggcgctgctg gcatcgggcg cagtgcagtt
     9361 gcccttctcg tgaatgtgaa atccgtgcgc tcccggcgtc aggccggtca cgtgcgcctc
     9421 caccagcacc agctcaccct gctgggtgaa gttgacggtg ccggccacct ggttgccttt
     9481 ggtgggagcc aggttcgcca ccgccgtggg cgagcgcagg ctcatgttgg cgcaggcggc
     9541 aagcgtcgcc acggcgaccg cagccaagac cgtgcgtggt gtcattgcat gctccccctt
     9601 tttttgaatg gcgagcgacg cgccgcactc gcccgcaccc ggtgcgtgcg cgagttgtag
     9661 cacccgacct cccccctgtc aaaccgattc cgtctcccgc caaaccggcc ccttttcaca
     9721 gcggatcggc tatgattcgc ccaacgtgac gacgcgtagc atgctggcta cccccccgcg
     9781 acctcgcccc gcgagtcagg gcagcgtcgc cccttccccg gtcagccgcc cgcactgatg
     9841 gcccaggctc ccggcaccgg aatcgccgcc ctcacccggg gtttcaccca actcagcaat
     9901 tctcagaagc tcggcttcct ggcggcgctg gccgcagtcg ccgcggtgat cgtggtcgcc
     9961 ctgctctggg ccggcgcgcc cgagtaccgc gtgttgtact cgaatctgtc cgagcgggac
    10021 ggtggcgaag tcatcggcgc gctggcgcag ctcgacgtcc cctaccgcgt gggcgaaggc
    10081 ggcagcgtcg tgctggtgcc cgcagggcag gtatacgagc tgcgcttgaa gctggccgct
    10141 cagggcctgc ccaaaggggg ggcggtcggc ttcgagctga tggacgggca gaagttcggc
    10201 ctcagccagt tcgccgagca ggtgaactac cagcgcgcac tcgccggcga gctggcgcgc
    10261 agcgtccagt ccatggtggc cgtacagtcg gcgcgcgtgc atctggcgat cccccggccc
    10321 accgtgttcg tgcgcgaggc gcgccagccg agcgcgtcgg tgttcgtggc cacgtatccc
    10381 gggcgcgcgc tcgacaccgg ccaggtggcg gcgatccagc acctggtggc gagcagcgtt
    10441 ccggagctgc cggcccgcaa cgtcaccgtg gtcgaccagg gcggcaacct gctctcccgt
    10501 ggcggcgccg aggcggccgg caacgcgctc gacgcgtcgc agctgcagta cctgcacacc
    10561 gtggagtcca gttacgcgcg gcgcatcgaa gcgatcctcg agccgatcgt ggggcgtgac
    10621 aacgtgcgcg cgcaggtcac cgcggcgctc gatttcagcc gctccgagca gacctccgag
    10681 acgttcaagc cgaacgcggc gtcggcggcc accatccgca gtcagcagac cacgatgcag
    10741 accgtgcctg ccagcggcgc gcagccgggc caggccgcgg ctgcgcgggc ggcggcgccc
    10801 ggcacacgcc gcgagagcgt cgtcagctac gaggtcgaca agaccgtgcg ccataccaag
    10861 ggcgaggtcg gcgcggtcaa gcggctgtcc gctgcggtgg tggtgaacta tcgcagggag
    10921 gcgggcgacg gcggcgctta ccagccgctc ggcgaggaga ccctgcagca gatcactgcg
    10981 ctcacacgcg aggcgatggg cttcgatccc gatcgcggcg acacgctgaa cctggtcaac
    11041 gcgccgttca acggcgaggt cgccgccggt gctgccggcg agagcacgct ggcggccccc
    11101 accggctgga tccaggacat cgccagccca gccggcatga cgttgttgaa gaccgcgctc
    11161 gtcgctctgg tgctgctcgc cgcgctgtgg cttgcgcgtt cggtgttgcg cgagctggcg
    11221 cgtgccgggc gcagatcgcc gggcagagcg ctgcccgggt cgggtgcgga agcacagctc
    11281 gccgccgccc cgcccgaggg cgcgggctac gaggccgatc tgcgcgcggt gcgggacatg
    11341 gcgcgccagg acccgcgcac ggtcgccaac gtggtcaggg aatgggtggc tcggcagtga
    11401 gggacggcgt cacgcgcagc gccatcctgc tgatgtcgct cggcgaggaa gaggcggtcg
    11461 aggtgttcaa gctcctcgct ccccgggagg tgcagaaggt cggccaggcg atggcccgac
    11521 tgggccgtgt ctcgcgcgac gacgtggcgg gcgtgctgcg cgatttccgc caggtggccg
    11581 agcagcaggt cccgatcggc accgcctcgg accagtatct gcgcagcgtg ctcacgcgcg
    11641 ccctggggga cgaccgcgcc gcgggcctga tcgaccgcat cctgcaggtc caggacaacc
    11701 gcggcatcga aggcctgaag tggatggacg cggcgtcggt cgcggaactg atccgcaacg
    11761 agcacccgca ggtcatcgcc accatactgg cgcacctgga cgcggaccag gcggccggcg
    11821 tgctgggcgc gctcgacgag cagctgcgcg gcgacgtcgt gctgcgcgtc gccaccctgg
    11881 agagcgtcca gccggcggcc atgcaggacc tcaacgaggt gctcgcgcaa ctgctgagcg
    11941 gcggcgagcg ggccggcaag agtgcgctgg acggcgtgca ggcggccgcc cagatcctca
    12001 atcgcatggg tccgatggag gcggcggtct gctcgcacat ccgcgaggtg gatgaggaac
    12061 tggcgcagcg catcgaagac aggatgctgg cgttcgacga cctgctcgag ctggatgacc
    12121 gcgacctgca gctgctgctg cgcgaggtgc cgtccgagac gctgttggtg gcgctgaagg
    12181 gcgccggcgc cgcgctgcag gacaaggtct tcaggaatct gtcacagcgc gccgcggaga
    12241 tgctgcgcga ggacctggag gccaagggcc cggtgcgcct gtcccaggtc gaagccgagc
    12301 aacgcgagat cctgcgcatc gcgcgccgcc tggccgcgga cgggctggtg gtgctgggtg
    12361 gcgcgggagg cgcgagcgtt gtcgggtagg atcatcccgc gcgagcaggc gcagcgcgcg
    12421 cgcaagctcg acctcggcga catcgccgcc ggccgctcgc cgatcgacac ccggcgggcc
    12481 ggcggcgagc aggacgcatc gcctgcggat atcgagcgcg tgcgcgagca ggcctaccgc
    12541 gaaggcgtgg aggccggcag gcgcgaggcg ggcgcacagc tcgcgcggca gcgcgacgag
    12601 ctgcgtgcgc tgattcaggg cgtcaacgag ttgctggaga acgtggagca gcgcctcgcc
    12661 accgaagtgc tgtccatcag tctggagctc gccaagctga tcgtgcgcgc cggcgtgcgg
    12721 gtcaggcccg agctggtgct gtcggtggtg caggatgcca tcgccagcct gccgggggcg
    12781 ggcgagcctg tcacgctgct gctcaacccc gccgacgccg cgctgctgcg cgagctggct
    12841 cgcgacgacc gggcgctgtc cgctctgccc tggaagctcc tggaggatcc gcacatcgag
    12901 cgcggaggct gcaagctgga gaccccgagc accgaggtgg acggcacgct ggagacgcgc
    12961 tggcgccgcg tgatcgccaa tctgggccgg gacgacgcct ggatagacat cacgacgtga
    13021 ggcccgagtc gcgatgagcc gcgtgcgccg ctggcagaag tacgtcgaag actgccgcac
    13081 ggcggtgcgc gctgcgcaac cgttcaccgt cagcggccgc ctcacgcgcg tggcaggact
    13141 ggtgatggaa gcggtcgggc tgaagctccc ggtgggcaac agctgttacg tggtcacgcc
    13201 cagcggccag cgcgtggacg ccgaggtggt gggcttttcc ggcgagcggc tgttcctgat
    13261 gccgtccacc gacgtctacg ggctcaagcc gggcgcgctg gtggtgccca tcgaccaggc
    13321 ggtgatgcgg gtgccgcggc tgggcgagga ctacgtgccg cggcggcgcg cgcaggatcg
    13381 cgccaagcac gtgccggtgg gcgccgggct gctcggccgc gtggtggacg gcgcgggccg
    13441 cccgctcgac aacgccgggc cgctggcgct ggaccgccac gtcccgctgc acagccggcc
    13501 gatcaacccg atggagcgtg cgccgatccg caccgtgctc gacgtcggca tccgcgccat
    13561 gaacgcgctg ctcaccgtcg ggcgcggaca gcgcctgggg ctgctctcgg gcagcggcgt
    13621 gggcaagagc gtgctgctca gcatgatggc gcgctacacc gccgccgacg tcgtcgtcgt
    13681 gggcctgatc ggcgagcgcg ggcgcgaggt gcgcgagttc atcgaaacca tcctcggcag
    13741 ggaggggctg gaacgctcgg cggtggtggc cgcgccggcc gacagcccgc cgctgctgcg
    13801 cctgcacggc gcggcctatg ccaccgccat cgccgagcac ttccgcgacc agggccggca
    13861 cgtgctgctg atcatggact cgctcacgcg ctacgccacc gctcagcgcg agatcagcct
    13921 ggccatcggc gagccacccg ccacccgggg ctataccccg tcggtgttcg ccaagctgcc
    13981 gcagctgatc gagcgcgccg gcaacggccc cgacaaccgc ggctcgatca ccgcgttcta
    14041 cacggtgctc accgaaggcg acgatctgca ggatccgatc gcggacgcgg cgcgcgcgat
    14101 cctggacggg cacatcgtgc tgtcgcgcga tctggccgac cagggtcact acccggcgat
    14161 cgatatcgag agctcgatca gccgcgcgat gaccgagctg ctgtccgcgg aagagctggc
    14221 gctggcgcgc cgcttcaagc agctgttcgc cgcctaccgg cgctcgcgcg acctgatcgc
    14281 ggtgggagcg tatgtgcgcg gcaacgaccc gctgctcgac cgcgccgtcg cgctgtatcc
    14341 gcagctcgcc gccttcctgc agcaggacat gtacgaacgg gcgagcgtgg cggcgagccg
    14401 cgagcgcctg cgcagcctgc tggaggagtg atggccgcgg ctttcgcgct gcagccgctg
    14461 ctcgacctgg ccgagcgccg cctggaagcg gccaccgccg agctgcagcg gctggcggcg
    14521 ttgcgacagc aggccgaggg cagcctcgcg cagctgcgcg ggcgccacgc gcagtacgcg
    14581 gccgaactgc agcgagcgct cgcgcgcggc atcgagccgg accggctgcg cgactatcgc
    14641 gccttcctcg ccagactcga gcgcgccgtc gccctgcagg ccgaagagct cgagcgccgc
    14701 cgccgggcgt gggccgacgc gcatgcgcgc tggctcgagc tgcgccggcg cgagcaggcg
    14761 ctcgccgtgc tcaggcgacg tcatcaactg gccgaggcgg tacgcgaagg ccggcgcgag
    14821 cagcgtcagc aggaggagct gatcagcagg aagagcggcc acgaggcgct ggaggacgtg
    14881 ccgctcgagg gcgcgctgcg caggtaaccc aactttggct gacgtggggc gcgcttgatt
    14941 tgctcttccg atcgacctac actccggcgc gaacatggct gtatttcctt caggttgttg
    15001 acttcctgtc gaccccgcta gatggccgac gacacgctct cgcaggaaga ggtcgaagcc
    15061 ctgctgcgcg gggtgaaggg cgcgcccggg caggtgcccg ctcccgccgc aatggatagc
    15121 gtgcgcagct acgacatcgg ccaccgccgg cgcatcgtgc gcgggggcat gcccgggttg
    15181 gagctggtca accagcgctt cgcgaacctg ctgcaggcgt cgctgtctcg cttgacgcgc
    15241 aggagcgcgg aggtcaccgc cgggccgccg caggtgctcg agtacagcga gctcgtgcgc
    15301 aacctgacga ccccggccag cctgagcctg gtgcaggcca agccgctgcg cggcgtcggc
    15361 ctcgtcgtgc tcgatgccga cctggtgtcc cagacgatcg atcagctgtt cggcggccag
    15421 gggcgcttcg ccgagcccgc gcagggcgcc cacgggcgcg agttcacggc cacggagctg
    15481 cgcgtcgcgc ggcgcctgct ggacgctgtt ctagaagcct acggcaaggc ctgggagccg
    15541 gtgtttcctc tgcgcttcga gcacctgcgg tcggagaccg atgcgcgttt cgccaacatc
    15601 gctgcgcccg atgaactggt ggtgaccagc agcttcagcg tcgatgcggg cctcgtcgat
    15661 gcgaaccccg tcgatgccaa ccggggcggc gcgcgatttc acgtgtgtct gccctatgct
    15721 atcctcgaac cgatccgcgg catcgtcgac cagccgatgc aggccgaacg cagcgacgcg
    15781 aacgaccgct ggacggcgct gctctcggcg caggtgcctg acatcgaagt cgagctggcc
    15841 gcgaacctgg cgcgcaccgc catcagcgtg cggcagctga tgcgactcga gagcggcgac
    15901 gtgatcgcga tcgagctgcc cgaggtgatc gcggccgaag tcgacggcgt cccggtgctc
    15961 gagtgcaagt acggcgtggt ccacgggcgc tacgcgctca aggtggtgcg cagggcacag
    16021 gatcgaggat ctgggagcta gatcctccac ggaggaacga tggcagacga tgacgtgaag
    16081 gccggcgtgg acgcgtgggc cccgctgggc tcgcaagcgg gctcgcagtc cgtgtcctcc
    16141 tccgccgccg gcagcggcgc gcgcgtgttc cccgaattga ccaccgagcc cggcgcgcgc
    16201 ggggccgccg ccagtctcga tatcgtcatg gacatcccgg tccagctctc ggtggagctg
    16261 ggccgcacca gactccccat ccgcaccctg gtgcagctgg cgcagggctc ggtagtggag
    16321 ctcgaccggc tggccggcga gcccctcgac gtgctcgtca acggctgcct gatcgcccag
    16381 ggcgaggtgg tcgtcgtcaa cgacaaactc ggcatccggc tcaccgaggt gatcgggccg
    16441 gacgagcgct tgcgccgtct gcagcggtga ccgcatgccg ttgagccggg ccatgcggcc
    16501 gccagtcgct cgctgggtcg cagggctcgc cgcgggtgta tccctcgcgc tgccggtcgc
    16561 cggcgccgat gctccggcgt cgggcacagc ggtaccggtg gggggcctgc tgcaagtggt
    16621 gttcgcgttg ctgctggttc tcggcgcgat cggcctgttc gcgtggctga tgcgccgctt
    16681 cgttcccggg cacggcaccg ccggcggcgt gctcaaggtg gtcggcgggg tgatggtcgg
    16741 gccgcgcgag cgactggtgg tggtcgaagt cggcgacacc tggttgttcg tcggcgtcgc
    16801 ggccagcagc gtgaggctgg tgcacagcat gccgcgcccc gagcgcagcg cggctgcacc
    16861 ggcgcaggcg acgtccttca cgcggcgctt gcggcatgcg ctggcgctac gccgcgggga
    16921 gggctgaccg cccgatgcga aaccaccccg cgagcaccgg cgcgccgctg tccggcacga
    16981 ccggatggcg ccgccccggc ggcggcgcgc aacgctggct gggtgttgtc ctgctgctcg
    17041 ccgccgccgc ggccgcgcgc gaggcgggcg cgcaggcgct gcccgcgatc accagcgcgc
    17101 cggcgcccgg cggcgggcag acctactcgc tcaccgtcca cacgctgctg ttcctgactt
    17161 cgctcgcatt cctgccggcg gtggtgctga cgatgaccgc gttcacgcgc atcatcatcg
    17221 tgctcgccct gctgcggcag gcgctgggaa cggtgcaggc gccgcccaac caggtgctcg
    17281 tcgggctggc gctgttcctg acctttttcg tcatggcgcc ggtgttcggg cgcatgtacg
    17341 acgaagccta cgtgccgctg tcggagcggc gcatcggcac ggagcaggcg ctggagcgcg
    17401 ccgccgtgcc gctgaggggg ttcatgctgc gccagacgcg acacgcggat ctggcgctgt
    17461 tcgcccagct cgccggcgag ccgccgccgg ccgacgccga cgcggtgccg atgcgcgtgc
    17521 tgctgccggc gtacgtgatc agcgagctga agaccgcgtt ccagatcggc ttcgtcgtgt
    17581 tcatcccgtt cctgatcatc gacctggtgg tggcgagcgt gctcatgtcg atgggcatga
    17641 tgatgctgtc gccggtgatc gtcgcgctgc ccttcaagct gatgctgttc gtgctggtgg
    17701 acggctggaa cctgctggtc ggttcgctgc tgcagagctt ctccgcgtga ctcccgagct
    17761 ggtcgtcgac ctggtgcagc aggcgctccg agtgggcatc ctggtggcgg cgccgatgct
    17821 gctcgcggcg ttgctcgccg ggctgatcgt gagcgtgttc caggccgcca cccagatcaa
    17881 cgagatgacg ctgccattcg tgcccaagct gctcgccctg ttcgccgccg cggtgctcgc
    17941 cggcccctgg atgctcgggc tgatggtgga cttcacccgg cgcctgttct cctcgattcc
    18001 gggcgtggtc gggtgacagc cggtggtcca gatcagcgcc ggcatgatcc agatcagcag
    18061 cgggcaatgg tatggctgga tcgccgcgtt cctgtatccg ctgttgcgcg tgctcgggct
    18121 gatcctggcc gacccgctgc tcggcaaccg cagcgtcccg ataacggcga agctggggct
    18181 cgcgctgctc atcgccgtgc tggtcgcgcc tctgcttccc ccggcaccgt cggcagaccc
    18241 ggcctccgct gccggcgtgt tgatcgccgc gcaacagctg ctgatcgggt tggcgatggg
    18301 cttctcgatc cgcgtcgcgc tcagcgcagc gcaaatggcc ggccagctcg ccgggctgca
    18361 gatgggcctc accttcgcgc agttcttcga cccgcacagc ggtgcgcaga gcgctgtcct
    18421 ggaccggttc ctgggattgt tcgcggtgct gatcttcttt tccatcgacg gccaccacgt
    18481 cgtgctgggt gtgctggtgg acagcttccg ggcgctgccg atcgccgccg cgccggtgga
    18541 cgcgctcggc ctgcgcgtgg tggtggagtg ggccgggacg atcttcaccg ccgggttgct
    18601 gatcgcgctg ccggtggttg ccacgctgct gatcgccaac gccgccctgg gaatcgtgat
    18661 gcgggccgcg ccgccgctga acctgttcac ggtgggcttc ccggtcacgc tcatcaccgg
    18721 gctgctggcg ctgtatctcg tggcgcccta cctcggcccg gcgctggcgg atctgttcga
    18781 gcaggccacg ctcgcgctgg ggcgcgtgct ggaagcgctg acgcccggct cgtgaccgtc
    18841 gaggcgcgcc ctgactgtca aggcgccccc ctgactgtca aggagccctt ggagtagact
    18901 atccgaccag ttcgcgcgct ctccgcgcgc gcgccgcctg gagacgacaa tgctcaaagg
    18961 cagtttggtg gcgatcgtgt cgccgatgca cgaggacggc agcctcgatc tcgagtcgtt
    19021 ccggggcctg gtcgactggc acatcgcaca gggtacggac ggcatcgtgg tggtgggcac
    19081 caccggcgag tcgccgaccg tggacttcga ggagcactgc ctgctgatca ggaccgcggt
    19141 tgagcgggcc ggcgggcgcg tcccggtcat cgccggcacc ggcgcgaact ccacgcgcga
    19201 ggcgatcgag ctgcaggcct tcgcgcacaa ggcgggggtc gacatgaccc tgtcggtagt
    19261 gccctactac aacaagccga cgcaggaggg cctgtaccgt cacttcaagg cgatcgccga
    19321 gtcggtggac ttgcccctga tcatgtacaa cgtgcccggc cgcaccgtgt gcgacatgca
    19381 caacgacacc gcgctgcgcc tggcgcaggt gcccaacatc gtcggcatca aggatgccac
    19441 cggcaacctc gagcgcggag cggatctcgt catgcgtgcg cccaaggatt ttgcggtcta
    19501 cagcggcgac gatgccacgg cgatcgcgct gatgctgct
//
LOCUS       DASPGE010000004         3964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1524175, whole genome shotgun sequence.
ACCESSION   DASPGE010000004 DASPGE010000000
VERSION     DASPGE010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524175"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VD791_00140"
     CDS             complement(<1..661)
                     /locus_tag="VD791_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV06412.1"
                     /translation="MAWKWLSALAAAVVVAAVALAGVRHWLLHSLTATAAPGSEVPVV
                     RTVTPEVAAASTDLRLPARTAPVEQALIYARATGVVSERRVDIGARVRRGDVLALIAA
                     PEIDQGVDKARAAVAQAKAKEQFATTNLQRSQQLVDQGFLSKQVLDERQAAFDAAQAD
                     RLAAAAELRRLNEIKGFQVVRAPFSGVIAERRVDRGDRVVGDQGNAEGYLFRLVRVNE
                     LR"
     gene            complement(689..3871)
                     /locus_tag="VD791_00145"
     CDS             complement(689..3871)
                     /locus_tag="VD791_00145"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV06413.1"
                     /translation="MWIVAYALRRRYTIGVLAILIVLFGLFSGRRMSTDILPPVDIPS
                     LNLVWIYSGLDAREMATRVTSFSELATINNVDNVREIRSETTAGLAVVKIDFQPYVNV
                     DTAMAQAVAVSQTILRRMPPGMQPPLIVRYSQSSTPILQLTLSSDTLSESQLFDFARI
                     QLRSQIQTIPGIRLTLPYGGAARQVMIDLNPEALQAYGLSAADVTRAVSAQNLVLPSG
                     VVREPGREMQVSVNASPAEVAAFQDLPIREVGGRVILLRDVAAVRDGSTVQTNVARLN
                     GQNGVIVSILKLGGASTVDIVDQVIARLPEIRAGAPKGLTIEPIFDQSVFVRAAIDAV
                     VKEAVLVALLVAAVVLVFLGSKRSTLIVLTSIPLALLASVAGLHAVGHTFNLMTLGGL
                     ALAIGILVDNALVEIENINRNLDQGKPLQQAILDSARQVAFPEFVSTLCICIVFIPVF
                     LLTGVASYVFAPLALSVVFAMAASYLLSRTLVPTLASLLLRDGGARRAEQAARRSTLY
                     LAHHAAEGVLEALRDTYRQRFARLLVHRLLLGLGCAAVIGVGVFAAWSLGREFFPAAD
                     AGLMRLYLRAPPGTRLEDTARQFAEVQRDIRAIVPAQELRFIGESIGAVEPINRGWVE
                     SVVVGSFDGELYIQLAPEHAPTARYMTRIRRMLKEKYPDLHAFFRPADNISQTLAGTS
                     PAALEVRFVGRAVPANLKLARELEQRLAQVPGAVDVGLLQVLDLPEYFIEVDRVRAMQ
                     IGVTQQEATNAILGVLGSGGTVAQSMWADGRMGISYNVQVHQPPISLTSLEQLLNTPV
                     RLSALGEPVLLRTIARASERRVPALLNRVTLAPSLNVVANVEGRDLGSVYHEVEALAG
                     ELRGKLEPGNRIEIVGQSKAMQSAYAELAGGIGIAAVLVFLVMVVNFQSWSAPAVALS
                     GLPFALTGAALALWATATPLSVPALMGMIMVVGVSTANSVLVTSFALSRLREGTGAVQ
                     AATEAAATRLRAVLMTASAMIVGVLPMALGLGEGGEQNAPLGRAVVGGLLLGTVATLV
                     VVPAVFALLMGGHRLAPLTRSGDAARPAVG"
BASE COUNT          655 a         1412 c         1333 g          564 t
ORIGIN      
        1 cccgcagctc gttcacgcgc accagccgga acaggtagcc ctcggcgttg ccctggtcgc
       61 cgaccacgcg gtcgccgcgg tccaccctgc gctcggcgat caccccggag aagggcgcgc
      121 gcaccacctg gaagcccttg atctcgttca ggcggcgcag ctcggccgcg gccgccaggc
      181 gatcggcctg ggcggcatcg aaggccgctt gccgctcgtc gagcacctgc ttcgacagga
      241 agccctggtc cacgagctgc tgagagcgct gcaggttggt ggtggcgaac tgctccttgg
      301 ctttcgcctg cgccacggcg gcgcgcgcct tgtccacacc ctggtcgatc tccggcgcgg
      361 cgatcagcgc cagcacgtcg ccgcgccgca cgcgcgcgcc gatgtccacg cgtcgttcac
      421 tcaccacgcc ggtggcgcgc gcatagatca gcgcctgttc cacgggcgcg gtgcgggcgg
      481 gcaggcgcag gtctgtgctc gccgcagcga cctcgggcgt gaccgtgcgc acgaccggca
      541 cctcgctccc gggcgcggcc gtcgccgtga gcgaatgcag cagccaatgc cgcacgccgg
      601 ccagcgcgac ggcggccacg acgaccgctg cggcgagcgc cgacagccac ttccatgcca
      661 ttgcgtttct cctgccggcg cgcatgcttt atccgacagc aggccgcgcc gcgtcacccg
      721 accgcgtcag cggcgcgagc cggtgcccgc ccatcagcag ggcaaacaca gccggcacta
      781 ccaccagtgt agcgacggtt ccgagcagca gcccgcccac cacggcgcgg cccagcgggg
      841 cattctgctc gccgccctcg cccaatccca gcgccatcgg cagcacgccg acgatcatcg
      901 cgctggccgt catcaacacc gcacgcaacc gcgtagcggc ggcctcggtc gccgcctgca
      961 ccgcgccggt gccctcgcgc aggcgcgaca gcgcgaagct ggtcaccagc acgctgttgg
     1021 cggtggacac gcccaccacc atgatcatgc ccatcagcgc cggcacgctg agcggcgtgg
     1081 cggtggccca cagcgccagg gcggcgccgg tcagcgcgaa cggcaggccg gacagcgcaa
     1141 ccgcgggcgc cgaccaggac tggaagttca ccaccatcac caggaacacc aggaccgcgg
     1201 cgatgccgat gccgccggcg agttcggcat acgccgactg catcgccttg ctctgaccga
     1261 cgatctcgat gcggttgccc ggttcgagct tgccgcgcag ctcgcccgcc agcgcttcca
     1321 cctcgtggta cacgctgccc aggtcgcggc cctcgacgtt ggcgaccacg ttgagcgacg
     1381 gggcaagcgt gacgcggttc aggagcgccg gcacgcgccg ctcgctggct cgcgcgatgg
     1441 tacgcagcag caccggctcg cccaatgccg acaaccgtac cggagtgttg aggagctgtt
     1501 cgagcgatgt cagcgatatc ggcggttgat gcacctgcac gttgtatgag attcccatgc
     1561 ggccatcggc ccacatgctc tgcgcgacgg tgccgcccga gccgagcacg ccgaggatcg
     1621 cattggtggc ctcctgctgg gtcacgccga tctgcatcgc gcgcacccgg tccacttcga
     1681 tgaagtactc gggcagatcc agcacctgca gcaacccgac gtcgaccgcg ccggggactt
     1741 gcgctaggcg ctgctccagc tcgcgcgcga gcttcaggtt ggcgggcacg gcacggccca
     1801 cgaagcgcac ctccagtgcg gccggcgagg tgcccgcgag cgtctggctg atgttgtcgg
     1861 ccgggcggaa gaaggcgtgc aggtcgggat acttctcctt cagcatgcgc cggatgcgcg
     1921 tcatgtatcg cgcggtcggc gcatgctcgg gcgcgagctg gatgtagagt tcgccgtcga
     1981 acgaccccac caccacgctt tccacccagc cgcggttgat cggctcgacc gcgccgatgc
     2041 tctcgccgat gaagcgcagt tcctgcgccg gtacgatggc gcggatgtcg cgttgcactt
     2101 ccgcgaactg ccgcgcggtg tcctccaggc gcgtaccggg aggcgcgcgc aggtaaaggc
     2161 gcatcaggcc ggcgtcggcc gccgggaaga actcgcgccc gagggaccag gctgcgaaca
     2221 ccccgacccc gatcacggcc gcgcagccca ggccgagcag aagcctgtgg acgagcagtc
     2281 gcgcgaagcg ctgtcgatag gtgtcacgca acgcctccag cacaccttcg gccgcatgat
     2341 gcgccaggta cagtgtgctg cggcgcgctg cctgttcggc ccgccgggct ccaccgtcgc
     2401 gcagcagcaa cgaggcgagc gtcggcacca gcgtgcgcga cagtaggtag gacgccgcca
     2461 tggcgaagac cacggacagc gcgagcgggg cgaacacgta ggaggccacg ccggtcaaca
     2521 ggaacaccgg gatgaagacg atacagatgc acagcgtgga gacgaactcg gggaaggcga
     2581 cctgccgggc gctgtcgagg atcgcctgct gcaacggctt gccctgatcc aggttgcggt
     2641 tgatgttctc gatctccacc agtgcgttgt ccaccaggat gccgatggcg agcgccaggc
     2701 cgccgagcgt catcaaattg aacgtgtgac ccacggcgtg cagcccggcc accgaggcca
     2761 gcagcgccag cgggatcgag gtcagcacga tcagcgtcga gcgcttggag ccgaggaaca
     2821 ccagcaccac cgcggccacc agcagtgcca ccagcaccgc ttccttcacc accgcgtcga
     2881 tcgccgcgcg cacgaacacc gactggtcga agatcggttc gatggtcagt cccttgggcg
     2941 caccggcgcg gatctccggc aggcgggcga tcacctggtc gacgatatcc acggtcgacg
     3001 ccccgcccag cttgaggatc gagacgatca cgccgttctg gccattcaac cgcgccacgt
     3061 tggtctgcac cgtcgagccg tcgcgcacag cggccacatc gcgcagcagg atcacacgcc
     3121 cgccgacttc gcggatcggc aggtcctgga acgcagcgac ctcggcgggg ctggcgttga
     3181 cgctcacctg catttcgcgc ccgggctcgc gcaccacgcc cgagggcagc accaggttct
     3241 gcgccgagac cgcgcgcgtc acgtcggcgg cggacaggcc gtaggcctgc agcgcctccg
     3301 gattgaggtc gatcatcacc tggcgcgcgg cgccgccgta gggcagagtg aggcggatgc
     3361 cggggatggt ctggatctgg ctgcgcagct ggatgcgggc gaagtcgaac agctgcgact
     3421 cggacaacgt atccgaggac aaggtgagct gcaggatcgg cgtgctcgac tggctgtagc
     3481 gcacgatcaa cggcggttgc atgcccggcg gcatgcggcg caggatggtc tgcgacaccg
     3541 ccaccgcctg tgccatcgcc gtgtccacat tcacgtaggg ctggaagtcg atcttcacca
     3601 ccgccaggcc ggcggtggtc tcggagcgga tctcgcgtac gttgtcgacg ttgttgatcg
     3661 tggccagctc gctgaacgag gtcacgcgcg ttgccatctc gcgcgcgtcc agcccgctgt
     3721 aaatccatac caggttgagc gaaggaatat ccaccggggg caggatgtcg gtggacatgc
     3781 gccgccccga gaacagcccg aacagcacga tcaggatcgc cagcacgccg atggtgtagc
     3841 ggcggcgcaa agcgtaggcg actatccaca ttgcctgttg ttccgatagt gcatgacaag
     3901 cgccacaccg ccgccggcct tcggccgacc cctcctcgtg agaggagggg agtggtcttc
     3961 aagt
//
LOCUS       DASPGE010000005        10076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1641682, whole genome shotgun sequence.
ACCESSION   DASPGE010000005 DASPGE010000000
VERSION     DASPGE010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10076
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641682"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="VD791_00150"
     CDS             complement(<1..120)
                     /locus_tag="VD791_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HXV06414.1"
                     /translation="MAATKPGERKLQILQTLAEMLETPSAERITTAALAAKLDV"
     gene            complement(125..754)
                     /locus_tag="VD791_00155"
     CDS             complement(125..754)
                     /locus_tag="VD791_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HXV06415.1"
                     /translation="MWIFDLDNTLHDANRHVFPHLNRSMTAYLERHLGLDAEGANALR
                     VDYWRRYGATLLGLMRHHRVEPRHFLRETHRFPELERAVLRHPLLRSVLTRLPGRKLV
                     FSNAPVHYSRAVLELLRVADLFDDVFTIEHTGFRPKPDPAGFLRLLRKHRLHPQRCIM
                     VEDDLANLMAAKRLGMKTVWVQPRGRARAHVDVTVSSLARLPRLLRRLR"
     gene            complement(793..1680)
                     /gene="argB"
                     /locus_tag="VD791_00160"
     CDS             complement(793..1680)
                     /gene="argB"
                     /locus_tag="VD791_00160"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HXV06416.1"
                     /translation="MALSDSSAGQRARILAEALPYIRRFYGKTIVVKYGGNAMTDERL
                     KGSFARDVVLLKLVGMNPVVVHGGGPQIDELLKRVGKKGEFVQGMRVTDAETMDVVEM
                     VLGGLVNKEIVTLINQHGGRAIGLTGKDGSFIRARKLLVPSGDNEAELLDIGQVGEVA
                     SIDPEIVALLETREFIPVVAPIGVGSDGRSYNINADLVAGKLAEVLRAEKLILLTNTP
                     GVLDKDGKLLTGLTPKQVDELFAAGVIHGGMLPKIGSALDAAKGGVKSVHIIDGRVEH
                     ALLLEVLTDEGVGTLIRNR"
     gene            complement(1910..2335)
                     /locus_tag="VD791_00165"
     CDS             complement(1910..2335)
                     /locus_tag="VD791_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXV06417.1"
                     /translation="MANITRWDPFEDIDDLFKGFFLRPMRVESGVDSQMRIKMDVKED
                     DKAYVVHAEVPGVKKEDIQVSIDGNQVSISAEVKREKEEKQGEKVLRTERYTGKVYRA
                     FSLAQDVDQDGARAKYENGVLELTLPKRAASAQRRLTVQ"
     gene            2519..3094
                     /locus_tag="VD791_00170"
     CDS             2519..3094
                     /locus_tag="VD791_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV06418.1"
                     /translation="MHTDADQLAQHRDYLVRFARVHLRDPARAEDVVQETLLAAMENR
                     AGFSGKAQLRTWLTGILKHKIIDVFRRQKRDAPDAGVDDLGPEADEAFADLYFDAGRE
                     DHWRSAPPAWGDPERSLEQKRFWEVFDACSKQMPAQTARVFLMRELMGLETEEICKEL
                     GITPNNCWVLLYRARMNLRQCLEARWFADRA"
     gene            3123..3350
                     /locus_tag="VD791_00175"
     CDS             3123..3350
                     /locus_tag="VD791_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXV06419.1"
                     /translation="MPDDADLDSAPAALTCKQVSVLLSRAQDTPLGALERLRLEAHLK
                     VCRGCENFRKQLDFLRRALRRHPALRDEEDR"
     gene            complement(3393..4559)
                     /locus_tag="VD791_00180"
     CDS             complement(3393..4559)
                     /locus_tag="VD791_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXV06420.1"
                     /translation="MTHFFADPFAWLAAAAGLACAGSLGWMLRERRRARRMEEAILSR
                     APAVSGPAAPRTDRVTAALEELDARVAAAQRDVRQQADELAKAEAALHESEERLRLAV
                     RGASDGLWEWNFRTRQAYFSSRAKAMLGYTDEELGNGVREWRALIHPDDLKAALGELE
                     AHIAGRSARFEFEHRMRHKDGSWRWVQARAAVVRHASGKPERLVGLYADVTARKRVQQ
                     LMVDLADGLSGLHGEACFRALVRSFAEVLGVREAFVCECCDYPTSRVRMLARWKNGDF
                     GRCVEFDLAGTACQDVIAGGQVLYQPQDAGVRWPLERTYERDSYLGLPCLDSQGRVIG
                     HIACADPGPMPGDLPQQAILKIFAVRAGLELERRELERERRAVGMRTAQHVALH"
     gene            complement(4556..6043)
                     /locus_tag="VD791_00185"
     CDS             complement(4556..6043)
                     /locus_tag="VD791_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV06421.1"
                     /translation="MFRTLYSKLAAVLLGLFALVGAGAVFGTLHVLSLYQLESSQRLN
                     RTLARHLADQNLLVNLSDANAQRLRGMFDMQMIINPAIQIYLLDGQGRIVAHSAAPGE
                     IKVERVSLGPLQAFLTPGGKFPVFGDDPRRIGPRRIFSAAPIPAHGPPQGYLYVVLAD
                     PVHERPASLLSQSYVIKLAVALALASVVLALLVGLAVFALITRKLRQLADGVERFQAT
                     DFSQRPRLPRLPEGRSGDEIDRLGVSLRDMAERIASQVQRLKQTDVLRRELVANVSHD
                     LKTPLATLQGYVDTLLLKDDALAPAERRHHLGVASRSCQRLSKLVADLIELAKLDAHE
                     VTPQLEPFSPGELLQDVAQKFQLKAEQRRVALEIVLPEGVPYVSADIGLIERVLENLI
                     GNALAHTDAGGTVRLTLDAAGSEVVLRVSDSGCGIPAQELPHVFERFYRVNGADWERG
                     GNAGLGLAITKSILDLHGSQMTVDSQVGVGTSFGFTLPAARLEVA"
     gene            complement(6082..6789)
                     /locus_tag="VD791_00190"
     CDS             complement(6082..6789)
                     /locus_tag="VD791_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXV06422.1"
                     /translation="MHRVLVIEDENDISQLIRLHLEDIGCEVKQAFDGIVGLAEAEAQ
                     GYDLIILDLKLPGMDGLEVCRRLRSRERYTPILMLTSKSGELDRVLGLELGADDYLTK
                     PFSVMELTARAKAIFRRIGMLGIRPPAQDDKLIAARELAIDIERHSVTVRGHSVDLTA
                     KEFDLLTWFARHPGRVYSREQLLDQVWGYQHSGYDHTVNSHINRLRAKIERDPQNPEY
                     ILTVWGVGYKFSEGARA"
     gene            complement(6876..>10076)
                     /locus_tag="VD791_00195"
     CDS             complement(6876..>10076)
                     /locus_tag="VD791_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HXV06423.1"
                     /translation="EIADGELDLRPAQGPALVLRNLDAALASRPDRIELNVRATSEPV
                     ERLTGRLRLAHAALEGGIELDLGGVRLAALQGFLPWRPDVYGIDGELDVRLLGTMRGA
                     SQLSVELDASAARLQLTAAGKPLTIEGAALKAVAAYARGNLNVTLDSLASTAPSLDSK
                     GVFSAGDDGYALRVQAVRLALGEWWPLLERYAPKLAEAVRIHAVPRQGAIDTIEVSAR
                     ADSLDELWRPERLTAAAAFEHVALDLPRFGVAVRELAGKAAYADGALRIEGLQGAVGA
                     SRIRSASGFVQLTGPRHPLQGRVELAVQLQEALRLARGAVRAEPARRQLARVRRLEGS
                     VLAQVELDGTLQAPLAAVVLSQPDFTALHEAMPYALAVSAGTARYDRGVLSVQGLAGR
                     LGGSTFSALTGMLGLQAPYPLRLSGGRADVALTELYRWLARQPAIASVLEGYSVRGGR
                     AAVSLASIAGALAEPERLRYRGEVIPSEVVVRARELEDTVRLDGGVLRIEPEALSASD
                     LHATTLGSALRVGGRLERVEDGFRLRQAEISGTLGQLLLDWLQARYDVPLQARPTAPL
                     QLDRVRLQTAPGEAFAAQGAVQSQDGARLEFDIRSETAGRVELNRIALRDAQSDATVR
                     ATLRDRHVHAAFAGVLAAATVRRLLPAAPVPFRTLRGDIEFDLDRDSPRASRALGWLQ
                     GEGLDAPFPANAPWRIERFSLEADGALLRIDSARIEGPGTDAVLSGTVAAAEDRYLVD
                     LQLQGKSVTAPAWGGKKTEAGQAAASNEGARGALPRLLAADLPVWGTVRVNLDRVAVG
                     QFEVMPLTAAGTLENGRLDLAVQRAALCGIALQARLAARPGQARLEGGLSSRGARLEE
                     SVPCLTERRVAATGGFDMDVRFTSEGPPGLLLDLIQGDFQFNARDGRILAFTTLNRVF
                     AALNVTEAVRGRLPDLTREGMAFKSAQVKGRIDGSRLVFEHSVLDADTVTLAAQGQAD
                     LAARTLDLDLLVAPLKTVDAVVRRVPILGRVLGGTLVAIPVRVRGPVTDPAVVPLAPQ
                     AVAARLLSILGNTLKLPVDLFDALGAAGQAPQGGTR"
BASE COUNT         1520 a         3627 c         3410 g         1519 t
ORIGIN      
        1 gacgtccagc ttggcggcga gcgccgccgt ggtgatgcgc tcggcgctgg gcgtctcgag
       61 catctccgcc agcgtctgca ggatctgcag cttgcgttcg ccgggcttgg tcgcggccat
      121 gggctcagcg caggcggcgc agcagccgcg gcagccgcgc gagactggag acggtgacgt
      181 ccacgtgtgc gcgcgcgcgg ccgcgcggct gcacccatac cgtcttcatg cccaggcgct
      241 tggcggccat caggttggcc agatcgtcct ccaccatgat gcagcgctgc ggatgcaggc
      301 gatgcttgcg cagcaggcgc aggaagccgg ccgggtccgg cttgggccgg aagccggtgt
      361 gctcgatggt gaacacgtcg tcgaacagat ccgccaccct gagaagctcc aataccgccc
      421 gggaataatg caccggcgcg ttcgaaaaca ccagcttacg cccgggcagc cgggtcagta
      481 ccgagcgcag cagcgggtgg cgcagcaccg cgcgctccag ctcggggaac cgatgcgtct
      541 cccgcaggaa atgccgcggc tcgacgcggt ggtggcgcat cagcccgagc agcgtggccc
      601 cgtagcgccg ccagtagtct acccgcaatg cgttcgcgcc ttcggcgtcg agccccaggt
      661 ggcgctccag gtaggcggtc atggagcggt tcaggtgcgg gaagacgtgc cggttggcgt
      721 cgtgcagcgt gttatccaga tcgaatatcc acaccgtgcc ggctccgcgg cgcggcctgc
      781 ggcgccggct catcagcggt tgcggatcag ggtgcccacg ccctcgtcgg tgagcacctc
      841 cagcagcagt gcgtgctcca cccggccgtc gatgatgtgc acgctcttca ccccgccctt
      901 ggcggcatcc agggccgaac cgatcttcgg cagcatgccg ccgtggatga cgccggcggc
      961 gaacagctcg tcgacctgtt tcggcgtcag gccggtgagc agcttgccgt ccttgtccag
     1021 cacgccgggc gtgttggtga gcaggatcag cttctcggcg cgcagcacct cagccagctt
     1081 gccggccacc aggtcggcgt tgatgttgta gctgcgcccg tcgctgccta cgccgatcgg
     1141 cgccacaacc gggatgaatt cacgcgtttc gagcagcgcc acgatctcgg ggtcgatgct
     1201 ggccacctcg ccgacctggc cgatgtcgag caactcggcc tcgttgtcgc cgctaggcac
     1261 caggagcttg cgcgcgcgga tgaaggagcc atccttgccg gtgagcccga tggcgcgtcc
     1321 gccgtgctga ttgatcaggg tgacgatctc cttgttgacc agtccgccga gcaccatctc
     1381 gaccacgtcc atggtctcgg cgtcggtcac gcgcatgccc tgcacgaatt cgcccttctt
     1441 gcccacgcgc ttgagcaact cgtcgatctg cgggccgccg ccgtgcacca ccacggggtt
     1501 catgcccacc agcttgagca gcaccacgtc gcgggcgaac gagcccttga ggcgctcgtc
     1561 ggtcatggcg ttgccgccgt acttgaccac gatggtcttg ccgtagaagc ggcggatata
     1621 gggcagggcc tcggccagga tcctggcccg ctggcctgcg ctggaatcgg agagcgccat
     1681 ggaacgattt tgggggaacg attttggggg aacggttttc gggggaacga gggctggggt
     1741 cgagggtggg tgcgcccggc gcaacggccg gtccgaaccg cggattctac cggtgcgcgg
     1801 gcgcgaccgg gcgcacgccc cggggacgcc gtccctcggt gcatgacgcc atcaccaagc
     1861 aaaaggcggc cgcaacgggc cgcctctcgc acgcggggat gctgcgctgt tactggaccg
     1921 tcaaccgtct ctgcgcggag gcagctcgct tcggcagggt cagctccagc acgccgttct
     1981 cgtacttggc ccgcgcgcca tcctggtcca catcctgggc caggctgaag gcgcggtaca
     2041 ccttgccggt gtagcgctcg gtgcgcaaga ccttctcgcc ctgcttctct tccttctcgc
     2101 gcttgacctc cgcgctgatg gacacctggt taccgtcgat cgaaacctgg atgtcctcct
     2161 tcttcacgcc cgggacctcg gcgtggacca cgtaagcctt gtcgtcctcc ttcacgtcca
     2221 tcttgatgcg catctgggag tcgacgccgc tctccacgcg catcgggcgc aggaagaagc
     2281 ccttgaacag atcgtcgatg tcctcgaagg gatcccagcg ggtgatgttt gccatagcca
     2341 ttgcctcctg tcgtatctgg tcagcctcgc agtgcaatca atcgggggcc cggtggccct
     2401 accgcgctat atgggcgcct gggggagcct ttcaagaggc cccggccccc gtgtcgtggc
     2461 ccgggcgcgc ttgtcccggc ccgccaaacg cactagattc cgctttggga cagaccgcat
     2521 gcacaccgac gccgatcagc tggcccagca ccgcgactac ctggtccgct tcgcccgggt
     2581 gcacctgcgc gacccggcgc gcgccgagga cgtggtgcag gagacgctgc tggccgcgat
     2641 ggagaaccgc gccgggttct cgggaaaggc ccagctgcgc acctggctta ccggcatcct
     2701 caagcacaag atcatcgacg tgtttcggcg ccagaagcgc gacgcgccgg acgcgggcgt
     2761 ggacgacctc gggccggagg cggacgaggc gttcgccgat ctgtacttcg acgccgggcg
     2821 cgaggaccac tggcgcagtg ctccgccggc ctggggggac cccgagcgat ccctggagca
     2881 gaagcgtttc tgggaggtgt tcgacgcctg cagcaagcag atgcccgcgc agaccgcccg
     2941 ggtgttcctg atgcgcgagt tgatgggact ggaaaccgag gaaatctgta aggaactggg
     3001 catcaccccg aacaactgct gggtgctgct gtatcgcgcc cgcatgaacc tgcgccagtg
     3061 cctggaggcg cgctggttcg ccgaccgcgc ctgagcctgc ctcgacccgg actgcccgca
     3121 ccgtgcccga cgacgccgat ctggattccg cccctgccgc gctgacctgc aagcaggtct
     3181 cggtgctgct gtcgcgggcc caggacactc cgcttggtgc tctggagcgg ctgcgcctgg
     3241 aggcgcacct gaaggtatgc cgcgggtgcg agaacttccg caagcagctc gactttctgc
     3301 gccgtgcgct gcgccggcac cccgccctgc gggacgagga ggacaggtag gcacagcctg
     3361 ccgaatctgt cggccggacg ccggcccttg cgtcaatgca gggccacgtg ctgcgcagtg
     3421 cgcatgccga ccgcgcggcg ttcgcgctcg agctctctgc gctccagctc cagccctgcg
     3481 cgcacggcga agatcttgag gatggcctgc tgcggcaggt caccgggcat cggcccgggg
     3541 tcggcgcatg cgatatggcc gatcacgcgc ccctggctgt ccaggcaggg caggcccagg
     3601 tagctgtcgc gctcgtaggt gcgctccagc ggccatctca cccccgcatc ctgcggctgg
     3661 tacaggacct ggccgcccgc gatcacgtcc tggcaggcgg tgccggccag atcgaactcc
     3721 acgcagcgcc cgaaatcgcc gttcttccaa cgcgccagca tgcgcacccg cgaggtgggg
     3781 tagtcgcagc attcgcacac gaacgcctcg cgcacgccca acacctctgc gaaagagcga
     3841 accagcgccc ggaagcaggc ttcgccgtgc aaccccgaca ggccatcggc cagatcgacc
     3901 atgagctgct gcacacgctt gcgcgcggtg acatcggcat acaggccgac caaccgctcc
     3961 ggcttgccgc tggcatgccg caccaccgcg gcgcgcgcct gcacccagcg ccaggaaccg
     4021 tccttgtggc gcatgcggtg ctcgaactcg aagcgcgcgc tgcggccggc gatgtgcgct
     4081 tccagctcgc ccagcgcggc cttgaggtcg tcgggatgga tcagcgcacg ccattcgcgc
     4141 acgccgttgc cgagctcctc gtccgtatag ccgagcatgg ctttcgcccg gctggaaaaa
     4201 tacgcctgcc gcgtccggaa gttccattcc cacagcccgt cgctggcgcc gcgcaccgcc
     4261 aggcgcaggc gctcttcgct ctcgtgcagg gccgcctccg ccttggccag ctcgtcggcc
     4321 tgctgccgga cgtcgcgctg ggccgcggcc acgcgcgcgt ccagctcttc cagggcggcg
     4381 gtgacccggt cggtgcgcgg cgcggccggt ccggaaacag cgggcgcgcg cgaaagaatc
     4441 gcttcttcca tgcgccgggc gcgccggcgc tcgcgcagca tccaccccag cgaccccgcg
     4501 caagcgagac ccgcggcggc ggcaagccat gcaaatgggt cggcgaagaa atgtgtcatg
     4561 ccacctccaa tctggccgcg ggcagcgtga agccgaacga ggtgcccacc ccgacctggc
     4621 tgtcgaccgt catctgacta ccgtgcagat ccaggatgct cttggtgatc gccagtccca
     4681 gcccggcgtt gccgcctcgc tcccaatcgg cgccgttcac ccggtagaag cgctcgaaca
     4741 cgtgcgggag ctcctgcgcc gggatgccgc agccggaatc gctcacgcgc agcacgacct
     4801 cgctgccggc ggcgtcgagc gtcaggcgca cggtgccgcc cgcgtcggtg tgcgcgagcg
     4861 cgttgccgat cagattctcc agcacgcgct cgatcaaccc gatgtccgcc gagacgtagg
     4921 gcacgccttc cggcaacacg atctccagcg cgacacggcg ctgctccgcc ttgagctgga
     4981 acttctgcgc cacgtcctgc agcagctcgc cgggcgagaa cggttcgagc tgcggcgtga
     5041 cctcatgcgc gtcgagcttg gccagctcga tcaggtcggc gaccagcttg gaaagccgct
     5101 ggcagctgcg cgaggccacg cccaggtggt gcctgcgctc ggccggcgcg agcgcgtcgt
     5161 ctttcagcag cagcgtgtcc acgtaaccct gcaaggtggc gagcggcgtc ttcaggtcgt
     5221 gcgacacgtt ggccaccagt tcccgccgca gcacgtcggt ctgcttgagc ctctgcacct
     5281 ggctcgcgat gcgctcggcc atgtcgcgca gactcacgcc caggcggtcg atctcgtcgc
     5341 cgctgcgtcc ctcgggcagg cgcggcagcc gcggacgctg gctgaagtcg gtggcctgaa
     5401 agcgctccac gccgtcggcg agctggcgca gcttgcgcgt gatcagcgcg aacaccgcca
     5461 gccccaccag cagcgcgagc acgacgctgg cgagcgccag cgcgaccgcc agcttgatga
     5521 cgtagctctg cgacagcagg ctggccggcc gttcgtgcac cgggtcggcc agcaccacgt
     5581 acagataacc ctgcgggggc ccgtgcgcgg ggatcggcgc cgccgagaaa atgcggcgcg
     5641 ggccgatgcg gcgcgggtcg tcgccgaaca ccggaaactt gccgcccggc gtcagaaacg
     5701 cctgcagcgg gccgagcgac acgcgctcga ccttgatctc gcccggtgct gccgagtgcg
     5761 cgacgatcct gccttgcccg tccagcagat agatctggat cgcgggattg atgatcatct
     5821 gcatgtcgaa catgccgcgc aggcgctgag cgttggcgtc gctcaggttc accagcaggt
     5881 tctggtcggc cagatgccgc gccagagtgc ggttgagccg ctggctggac tccagctggt
     5941 acaggctcag cacgtgcagc gtgccgaaca ccgcccccgc tccgaccaga gcgaacaggc
     6001 cgagcaggac ggcggcgagc ttgctgtaga gagtgcggaa catgctgcga acgaccagcc
     6061 cttggcgcta cacctggggc gctaggctcg cgctccttcg gagaacttgt accccacccc
     6121 ccacaccgtg aggatgtact cggggttctg cgggtcgcgc tcgatcttgg cgcgcaggcg
     6181 gttgatgtgc gagttcaccg tgtggtcgta gccgctgtgc tgatatcccc acacctggtc
     6241 gagcaactgc tcgcggctgt agacgcgccc cgggtggcgc gcgaaccagg tgagcaggtc
     6301 gaattccttg gcggtgagat cgaccgaatg cccgcgcacg gtgacgctat ggcgctcgat
     6361 gtcgatggcc agctcgcggg cggcgatcag cttgtcgtcc tgcgccggag gccgtatgcc
     6421 gagcatgccg atgcgccgga agatggcctt ggcgcgggcg gtcagctcca tcacgctgaa
     6481 cggcttggtg aggtagtcgt ccgcacccag ctccaggccc agcacgcggt cgagctcgcc
     6541 cgacttggag gtcagcatca ggatcggcgt atagcgctcc cggctgcgca gccgccggca
     6601 cacctccagg ccgtccatgc cgggcagctt cagatcgagg atgatcaggt cgtagccctg
     6661 cgcctcggcc tcggccagcc cgacgatgcc gtcgaaggcc tgcttgacct cgcagccgat
     6721 gtcctccagg tgcagccgga tgagttgcga gatgtcgttc tcgtcttcga tcaccaggac
     6781 gcgatgcatg gctcccccga tgcggatgtc ccggcactgc gtcggcggag gtgccgcttt
     6841 ccttacaccg tccagggcgg tgcgagaaat gctcgctagc gcgtgccgcc ctgaggggcc
     6901 tggccggcgg cgccgagggc gtcgaacaga tccaccggca gtttcagggt gttgccgaga
     6961 atgctcagca ggcgtgcggc gaccgcctgc ggggcgagcg gcacgaccgc cggatcggtc
     7021 accgggccgc gtacgcgcac cgggatcgcc accagcgtcc cgcccagcac gcgccccagg
     7081 atcggcaccc gccgcaccac ggcgtccacg gtcttcaacg gcgccaccag cagatccagg
     7141 tccagggtgc gcgcggcgag gtccgcctgc ccctgcgccg ccagcgtgac ggtgtcggcg
     7201 tccagcacgc tatgctcgaa caccaggcgg ctgccgtcga tccgcccttt cacctgggcg
     7261 gatttgaagg ccatcccctc gcgggtcaag tcgggcaggc gcccgcgcac cgcctcggtc
     7321 acgttgagcg cggcgaacac gcgattgagc gtggtaaagg caaggatgcg tccgtcccga
     7381 gcgttgaact ggaagtcgcc ctggatgagg tcgagcagca ggcccggcgg gccttccgag
     7441 gtgaagcgca catccatgtc gaagccgccg gtggcggcga cgcggcgctc ggtgaggcag
     7501 ggaacggact cctccaaccg cgcaccgcgg ctggacagcc cgccttccag ccgcgcctgg
     7561 ccaggccgcg ccgcaagccg cgcctgcagc gcgatgccgc acagcgccgc gcgttgcacg
     7621 gcgaggtcga ggcggccgtt ctcgagcgta ccggcggccg tgagcggcat cacctcgaac
     7681 tgaccgaccg cgacgcgatc cagattcacc cgcacggtgc cccagaccgg aaggtccgcc
     7741 gcgagcagcc ggggcaacgc cccgcgcgcg ccctcgttcg atgccgcggc ctgccccgct
     7801 tcggtttttt tcccgcccca ggcgggcgcc gtgaccgatt ttccctgcag ctgcagatcc
     7861 acgaggtagc ggtcttctgc ggcggccacg gttccgctca gcaccgcgtc ggtgcccggc
     7921 ccctcgatgc gggccgagtc gatgcgcagc agcgcgccat cggcctcgag cgagaagcgc
     7981 tcgatgcgcc agggggcgtt cgcggggaac ggcgcatcga ggccctcgcc ctgcagccac
     8041 cccagcgccc ggctcgcgcg cggagaatcg cgatccaggt cgaactcgat gtctccgcgc
     8101 agcgtgcgaa acggcaccgg cgcagcgggc agcaggcggc ggacggtcgc ggcggcgagc
     8161 acgcccgcga acgccgcgtg cacatgccgg tcgcggagcg tggcgcgcac tgtcgcgtcg
     8221 gactgggcgt cgcgcaaggc gatgcggttc agttcgacgc ggccggccgt ctcgctccgg
     8281 atatcgaact ccagccgcgc cccgtcctgc gactgcaccg cgccttgcgc ggcgaatgct
     8341 tccccgggcg ccgtctgcaa gcgcacgcga tcgagctgca gcggcgctgt cggtcgcgct
     8401 tgcagcggta cgtcgtagcg cgcctgcagc cagtcgagca gcagctggcc gagcgtgccg
     8461 ctgatctccg cctggcgcag ccgaaagccg tcctcgaccc gctccaaccg cccgcctacc
     8521 cgcaaggccg aaccgagtgt cgtggcatgc aggtcgctag cgctcagcgc ttcgggttcg
     8581 atacgcaaca cgccgccgtc cagtcgcacc gtgtcctcca attcgcgcgc gcggaccacg
     8641 acttcgctgg gaatcacctc gccgcggtag cgcagccgct ccggctcggc cagcgcgcct
     8701 gcgatcgacg cgagcgacac cgccgcccgg ccgcctcgca cgctgtagcc ctcgagcacg
     8761 gatgcgatcg cgggttgccg cgcgagccat cgatacagct cggtgagcgc gacatcagcg
     8821 cggccgccac tgagccgcag ggggtatggc gcctgcaacc cgagcatgcc ggtgagcgca
     8881 ctgaaggtcg agccgcccaa ccgcccggcc aggccttgca ccgagagcac gccgcggtcg
     8941 tagcgcgccg tgcctgccga caccgcgagc gcatagggca tcgcttcgtg caaggcagtg
     9001 aagtcgggct gcgacagcac cacggccgcc agcggcgcct gcagcgtccc gtccagctcg
     9061 acctgggcga gcacgctccc ctccagccgc cgcacgcgtg cgagctggcg ccgtgcaggc
     9121 tcggcgcgca ccgctccgcg ggccaggcgc agcgcttcct gcaactgcac cgccagctcg
     9181 acgcggccct gcagcggatg cctcggtccg gtgagctgga cgaagccgga ggcgctgcga
     9241 atgcgcgacg cgccgaccgc gccctgcaag ccttcgatgc gcagcgcgcc gtcggcatag
     9301 gctgccttcc ccgccagctc ccgcacagcg acgccgaagc gcggcagatc gagggccaca
     9361 tgctcgaacg cggcagctgc cgtcaggcgc tccgggcgcc aaagctcgtc cagcgaatcc
     9421 gcacgcgcgc tcacttcgat cgtgtcgatc gcaccctggc gcggaacggc atggatccgg
     9481 accgcctctg ccagcttcgg cgcgtaccgt tcgagcagcg gccaccactc acccagcgcc
     9541 aggcgcacgg cctgcacgcg caacgcgtaa ccgtcgtcgc cggcgctgaa cacacccttc
     9601 gaatcgaggg acggcgccgt gcttgccagc gaatcaagcg tcacgttcag attgccgcgc
     9661 gcgtacgccg ccacggcctt gagcgcagcg ccctcgatgg tcagcggctt tcccgcagcg
     9721 gtgagctgga ggcgcgcggc gctggcgtcg agctcgacgc tgagctgcga cgcgccacgc
     9781 atcgtaccga gcaggcgcac gtctagctcg ccatcgatgc catagacatc cggtcgccag
     9841 ggcaggaaac cctgcaacgc ggcgagacgc acgccgccga ggtcgagctc gattccgccc
     9901 tccagcgccg cgtgcgccag ccgcagccgg cctgtgaggc gctcgaccgg ctcggaggtc
     9961 gcgcgcacgt tcagctcgat ccgatcgggt cgcgacgcga gcgccgcgtc gagattgcgc
    10021 agcaccaacg ccgggccctg cgccggccgc agatccagct cgccgtcggc gatctc
//
LOCUS       DASPGE010000006         4839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1056539, whole genome shotgun sequence.
ACCESSION   DASPGE010000006 DASPGE010000000
VERSION     DASPGE010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056539"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VD791_00200"
     CDS             <1..211
                     /locus_tag="VD791_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06424.1"
                     /translation="ASPEKLAKFCGCLGDLADAHGIPFGREQVSGYFDAIAAQGYPVV
                     RHSDAYRAAYRPAYRVVLRELLSDV"
     gene            260..1456
                     /locus_tag="VD791_00205"
     CDS             260..1456
                     /locus_tag="VD791_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXV06425.1"
                     /translation="MKITAIETIRLPDRPNLLLAQIHTDAGLIGLGETSRGAAAVEAQ
                     IHELVAPALLGKDPLAIQQHSRQLLNSYLGFASAGVEVRAASVVDIALWDIFGQAVNQ
                     PITQLLGGACRDRIRAYNTCAGYHYNTRTVARRQITAQDTPEGPYDDQIAFTQRADEL
                     AESLLSEGFTAMKIWPFDPLAERNAGSFLSREDLELGLAPWRRIRKAVGDRIEVMAEF
                     HSLWSLQQAKQIARALEDFRPYWSEDPIKMCDVATLKEYARSTSIPVCGSETLGGTAP
                     FRDMLQAQAADIVMLDVGWCGGLTEARKIAALAESYQRPVAPHDCNGPVVWVASIHLM
                     MHIPNALIMEAVRAYYSTWYQDVITEVPAVKGGYIYPLTGPGLGTKLLPGLPKREGVQ
                     VRRSPL"
     gene            1546..2700
                     /locus_tag="VD791_00210"
     CDS             1546..2700
                     /locus_tag="VD791_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXV06426.1"
                     /translation="MPAENLRIVDIKAIPTSFPVDPKNSVTLGIGRAVKRDAVLVKVS
                     TDAGIVGWGEAHHGRAPGSVAHLINNTLKQLVLGQDAYDVVGVWQRVYVKQLGSHGMG
                     AAAAIGLSGIDQALWDIRGKAAGLPLYKLLGGRSQRIPAYAGGVSLGYQEPQALVAEA
                     QPLVAAGYKALKLRVGDTPARDLARVAAVRKALGDDIAILSDANTGYSVADVRRVMAG
                     FEAHGVGWLEEPFPPHDYASYAQAASFGNVPLAAGENHYTRFEFHRMIEDGVITVLQP
                     DLSKSGGVTECLRIAHLASAWKLPINPHTSMTGINMAVTIHFLCAIDNAGYFEGDVSK
                     GNRFRDELVATPYTVGSDGCVAPLEKPGIGVEVDEKFLEAHPVIEGPAYI"
     gene            2755..3195
                     /locus_tag="VD791_00215"
     CDS             2755..3195
                     /locus_tag="VD791_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06427.1"
                     /translation="MKTSNLLAGAVLLLGCAAGAQAADGGIPAETQARLFQPVPDKAV
                     IYLLRDRGDLWRFGIRVLLDGRDMGETSPNSYFRWEVAPGPHVIVSDTTPPAVLELST
                     QPGGLYYVWQDINVGFLRAESRLEEVNAYTAKQTMDSAVLLTGK"
     gene            complement(3239..4456)
                     /gene="chrA"
                     /locus_tag="VD791_00220"
     CDS             complement(3239..4456)
                     /gene="chrA"
                     /locus_tag="VD791_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HXV06428.1"
                     /translation="MSSAAPSVHPRASALEVLLVFLKLGLTSFGGPVAHIGFLREELV
                     ARRRWLDEHTYADLVALCQFLPGPASSQVGMSIGQLRAGIPGAIAAWVGFTAPSAIAL
                     LLFAYGMHALRDVLHEGVLHGLKVVTVAVVAQAVWAMGRALCTDRTRVVIAALAAGAC
                     LWRPGSLTQVAVLFAAGVAGWRLLPVAALAGAETIAIPATRSIAIGAWVGFFALLLLL
                     PVAAKLSGPGALAMFDSFYRAGALVFGGGHVVLPLLEAEVVAPGWVSRDDFLAGYGAA
                     QAVPGPLFTFAAYLGAVMRVEPSGWVGSLLCLTAIFLPAFLLVLGALPFWQQLRGNRS
                     VRAALAGVNAAVVGLLLAALYDPVAVGGITSVRDAALALAAFALLWWRVAPWLVVLLT
                     AAASGMIYALIGS"
     gene            4619..>4839
                     /locus_tag="VD791_00225"
     CDS             4619..>4839
                     /locus_tag="VD791_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXV06429.1"
                     /translation="MPLAARICAVALAAVCAPGAAGAADFPSKPLRIIVPFPPGGFND
                     QLARILGQKFTARWRQPVVVDNRAGGSTLV"
BASE COUNT          831 a         1717 c         1632 g          659 t
ORIGIN      
        1 cgcctcgccc gagaagctcg ccaagttctg cggctgcctg ggcgacctcg ccgacgccca
       61 cggcattccc ttcggccgcg aacaggtgag cgggtatttc gacgcgatcg ccgcgcaggg
      121 ctacccggtg gtacggcact ccgacgcgta tcgcgccgct taccggcctg cttaccgcgt
      181 ggtcctgcgc gagctgctat cggacgtgta gcccggatgg agcgtaagcg gaatccgggg
      241 acacgataca tgcttcgcca tgaagatcac cgcgatcgag accatccgcc tgccggaccg
      301 gcccaacctg ctgctcgcgc agatccacac cgatgcgggt ctgatcggcc ttggcgaaac
      361 gtcccgcggc gcggccgccg tcgaggcgca gatccacgag ctggttgcac ccgccttgct
      421 cggcaaggac ccgctcgcca tccagcagca cagccgccag ctcctgaaca gctatctggg
      481 cttcgccagc gccggcgtcg aagtgcgcgc ggcctcggtg gtggacatcg cgctgtggga
      541 catcttcggt caggcggtga accagcccat cacccagctg ctcggcggcg cctgccgcga
      601 ccgcatccgc gcctacaaca cctgcgccgg ctaccactac aacacgcgta ccgtcgcgcg
      661 gcggcagatc acggcacagg acacgcccga gggtccgtac gacgaccaga tcgccttcac
      721 gcagcgcgcc gacgagctgg ccgagagcct gctgtccgaa ggcttcacgg cgatgaagat
      781 ctggcccttc gatccgctcg ccgagcgcaa cgccggcagc ttcctgtcgc gggaggacct
      841 ggagctgggc ctcgcgccgt ggcgcaggat ccgcaaggcc gtgggcgatc gcatcgaggt
      901 gatggcggag ttccactcgc tgtggagcct gcagcaggcc aagcagatcg cccgggcgct
      961 ggaggacttc cgcccctact ggtcggagga tccgatcaag atgtgcgatg tcgctacgct
     1021 caaggagtac gcccggtcga cgtcgattcc ggtgtgcggc agcgaaacgc tcggcggcac
     1081 ggcgcccttc cgcgacatgc tgcaggcgca ggccgccgat atcgtcatgc tcgacgtggg
     1141 ctggtgcggg gggctgaccg aagcgcgcaa gatcgccgcg ctcgccgaaa gctaccagcg
     1201 tccggtcgcg ccgcacgact gcaacggccc ggtggtgtgg gtcgcctcca tccacctgat
     1261 gatgcatatc cccaacgcgc tgatcatgga agcggtgcgc gcctactaca gcacctggta
     1321 ccaggacgtg atcaccgagg tgccggcggt gaagggcggc tacatctacc ccctcaccgg
     1381 accgggcctg ggcactaagt tgttgccggg cctgccgaag cgggagggtg tgcaggtcag
     1441 gcgcagcccg ctttgagccc tacccccgga ttccgcttcg ctgcatccgg gctacactct
     1501 gcttccggac tgcattgggc gagaaacaac cgcggaggac aagcgatgcc cgccgaaaac
     1561 ctgcgcatcg tcgacatcaa ggcgattccg acctcttttc cggtcgaccc caagaacagc
     1621 gtcacgctcg gcatcggccg tgcggtgaag cgcgatgcgg tgctcgtcaa ggtcagcacg
     1681 gatgccggca tcgtcggctg gggcgaggcg catcacgggc gggcgccggg ctcggtcgcc
     1741 catctgatca acaacacgct gaagcagctc gtactgggtc aggatgcgta cgacgtggtc
     1801 ggcgtgtggc agagggtgta cgtaaagcag ctcggcagcc acggcatggg cgcggccgcg
     1861 gccatcgggc tgtccggcat cgaccaggcg ttgtgggaca tccgcggcaa ggccgcgggc
     1921 ctgccgctct acaagctgct gggcgggcgc tcgcagcgca tcccggctta cgccggcggg
     1981 gtctcgctcg gctaccagga gccgcaggcg ctggtggccg aggcgcagcc gctggtagcc
     2041 gccgggtaca aggcgctcaa gctgcgcgtg ggcgatacac cggcgcgcga tctggcacgg
     2101 gtggccgcgg tgcgcaaggc gctcggcgac gacatcgcca tcctgagcga cgcgaacacc
     2161 ggctacagcg tcgccgacgt gcgccgggtc atggcgggtt tcgaagcgca cggcgtgggc
     2221 tggctggagg agccgttccc gccccacgac tacgcgagct acgcgcaggc cgcatcgttc
     2281 ggcaacgtgc cgctggccgc cggcgagaac cattacacgc gcttcgagtt ccatcgcatg
     2341 atcgaggacg gagtcatcac cgttctgcag cccgacctgt cgaaatccgg cggggtcacc
     2401 gaatgcctgc gcatcgccca cctcgcctcc gcgtggaagc tgccgatcaa cccgcacact
     2461 tccatgaccg gcatcaacat ggcggtcacg atccatttcc tgtgcgccat cgacaacgcc
     2521 ggctacttcg aaggcgacgt gtccaagggc aaccgtttcc gcgacgagct cgtcgccacg
     2581 ccctacacgg tgggcagcga cggctgcgtc gcgccgctgg agaagccggg catcggcgtg
     2641 gaggtggacg agaagttcct cgaggcccac ccggtcatcg aaggaccggc ctatatctaa
     2701 cctggcgcgc agtgtgcttg ccgcagcctg cacgacatcg atcaggaggc gatcttgaag
     2761 acatcgaact tgctcgcggg agccgtcctg ctgctcggct gcgcagcggg cgcccaagcg
     2821 gcggatggcg gtatccccgc cgagacccag gcgcggctgt tccaacccgt cccggacaaa
     2881 gcggtgatct acctgctgcg cgaccgcggt gacctctggc gcttcgggat ccgggtcctg
     2941 ctcgacgggc gcgacatggg tgagacctcg ccgaacagct acttccgctg ggaagtggcg
     3001 cccggcccgc atgtgatcgt gtcggacacc acccccccgg cggtactcga gctcagcact
     3061 cagcccggcg ggctgtacta cgtttggcag gacatcaacg tcggcttcct gcgcgcggag
     3121 tcgcggctgg aagaggtcaa cgcgtacacc gcgaagcaga cgatggactc ggcggtgctg
     3181 ttgaccggga aatagatcct catccgagcg caaagtgcgt agcccggatg catccgggct
     3241 acgaaccgat cagggcgtag atcattcccg acgccgctgc ggtcagaagg acgaccagcc
     3301 acggcgccac gcgccaccac agcaacgcga aagccgcgag cgccagcgcg gcgtcgcgca
     3361 cgctggtgat cccgcccacg gcgaccggat cgtagagcgc cgccagcagc agccccacca
     3421 cggcggcgtt taccccggcg agcgccgcac gcacgctgcg gttgccgcgc agctgctgcc
     3481 agaacggcag cgcccccagc accagcagga atgccggcag aaagatggcc gtcaagcaca
     3541 gcaggctccc gacccagccg ctcggctcga cgcgcatcac cgctcccagg taggcggcga
     3601 aggtgaacag cggccctggc accgcctgcg ccgcgccata tcccgccagg aaatcgtcgc
     3661 ggctcaccca gccgggcgcc accacttccg cctcgagcag cggcagcacg acgtgcccgc
     3721 cgccgaacac cagcgcgccg gcgcggtaga agctgtcgaa catcgccagc gcccccgggc
     3781 cgctgagctt cgcggcgacc ggcaacaaca gcaacagcgc gaagaagccc acccaggcgc
     3841 caatggcgat cgagcgcgtg gcgggaatcg cgatcgtctc cgcgccggcc agcgcagcga
     3901 ccggcagcag ccgccagccg gccacaccgg cggcgaaaag cacggccacc tgcgtgagcg
     3961 atcccggacg ccacaggcaa gcgccggcgg ccagcgcggc gatgactacg cgcgtgcgat
     4021 cggtacacag cgcgcgtccc atggcccaca ccgcctgcgc caccaccgcc accgtcacca
     4081 ccttcaggcc atgcagcacg ccctcgtgca ggacatcgcg caacgcatgc atgccgtagg
     4141 cgaacagcag cagggcgatc gccgacggcg cggtgaaccc gacccaggcc gcgattgcgc
     4201 cggggatgcc ggcgcgcagc tggccgatgg acatgccgac ctggctgctc gcggggccgg
     4261 gcaggaactg gcacagcgcc accaggtccg cgtaggtgtg ctcgtccagc cagcggcggc
     4321 gcgcgaccag ctcttcgcgc aggaagccga tgtgcgccac cgggccgcca aaggaagtga
     4381 ggcccagttt caggaacacc agcagcacct cgagcgccga tgcgcgcggg tggacggagg
     4441 gggcagcgga agacatgggc cgagggtggt cgacgccgga actgtacacc agcgcccccg
     4501 gccgctgcgc aggtgagccg ctggtgtatc cttcgggcgc ggccggcagc tcgatggccc
     4561 gaagcccggt catcgccaca cgagcaaacg gggccgcgcg aggaggagat cgacctgcat
     4621 gccgctcgct gcacggatct gcgccgtcgc gctcgccgcc gtgtgcgcgc cgggagccgc
     4681 gggggctgcc gactttccga gcaagccgct gcgcatcatc gtgccgttcc cgcctggcgg
     4741 cttcaacgat cagctggcgc gcatcctggg ccagaagttc accgcgcgct ggcgccaacc
     4801 ggtcgtggtc gacaaccgcg ccggcggaag taccctggt
//
LOCUS       DASPGE010000007         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_470990, whole genome shotgun sequence.
ACCESSION   DASPGE010000007 DASPGE010000000
VERSION     DASPGE010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470990"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VD791_00230"
     CDS             <1..578
                     /locus_tag="VD791_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV06430.1"
                     /translation="VTFGLAIVIQNLLQEIYSADPRSIESGGLNTASVSLGGGLAVGT
                     LPLLIFALAVAMAIGMQLLFDRTPIGRAFRAVSDDKDAAELMGLDHRHVYALATGIAF
                     LLVAVAGTLHGMKTTVSPADGPALLLYAFEAVIIGGMGSFYGTLAGGIILGVTQAIGF
                     RFDPGWGIWFGHIVFLAMLVFRPSGLFPKTR"
     gene            582..1619
                     /locus_tag="VD791_00235"
     CDS             582..1619
                     /locus_tag="VD791_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV06431.1"
                     /translation="MTPHALTATQFDVSRATTASRAGAMLGVLLLATLATFPFWADAG
                     WMREFNEMACYLIFAMMWNLLAGYGGMVSIGQQAYLGLGGYALLVMGNFFGVNPFVAV
                     PIAAVLTMLVALPVSQIAFRLQGGYFAIGTWVIAEVFRLSVANVSAVGGGSGTSFTAL
                     RGIDKVTRQSVTFWLCLVGAVASVALVYWFLRSRQGLALLAIRDSEIASESQGVNVRA
                     TKLGVYLVAAFGAGLAGALYFLGNLRISPDAAFTVNWTAFSIFIVLIGGIGTIEGPII
                     GVILFYLLSKFFSDFGTWYLVGLGLLAIVVTVRFPRGLWGWLSQRFDLHFFPVQRRLV
                     LREPDKETSHG"
     gene            1612..2817
                     /gene="pcaF"
                     /locus_tag="VD791_00240"
     CDS             1612..2817
                     /gene="pcaF"
                     /locus_tag="VD791_00240"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HXV06432.1"
                     /translation="MAEAFLCDGVRTPIGRYGGALSSIRTDDLAALPIKALMQRHPDL
                     DWQALDDIVYGCANQAGEDNRNVARMAGLLAGLPVEVSGCTVNRLCGSGMDAINIAAR
                     AIKSGEAELMVAGGVESMTRAPFVMSKADSAFSRTAQVFDTTIGWRFVNKLMKEKHGV
                     DSMPETADNVAADFKISREDQDKFALRSQQRWHRAKEAGKFKEEIVPVAVPQRKGDPV
                     MFDTDEHPRPDTTLEALAKLKGINAPELSVTAGNASGVNDGACALIVASETAVKRHGL
                     RPIARVAGTATAGVPPRIMGFGPAPASRKLLARLGLEVQDMTVLELNEAFASQVLAAL
                     RDLGVPDDAEDVNPNGGAIAVGHPLGMSGARLVMTAAYELRRRNGRYALATMCIGVGQ
                     GIAMVIERA"
BASE COUNT          415 a          954 c         1029 g          488 t
ORIGIN      
        1 tggtgacctt cggcctggcg atcgtgatcc agaacctgct gcaggaaatc tacagcgccg
       61 atccgcgctc gatcgagtcg ggcggcctga acacggcgag cgtgtcccta ggcggcgggc
      121 tggcggtggg cacgctgcca ctgctgattt tcgcgctcgc ggtggcgatg gcgatcggca
      181 tgcaactgtt gttcgaccgc accccgatcg gccgcgcgtt ccgggcggtg agcgacgaca
      241 aggacgcggc cgagctgatg gggctcgatc accggcacgt gtacgcgctg gccaccggca
      301 tcgcattcct gctggtggcg gtggccggta cgctgcacgg catgaagacc acggtatccc
      361 cggccgacgg gccggcgttg ctgctctatg cgttcgaggc ggtgatcatc ggcggcatgg
      421 gctcgttcta cggcacgctc gccgggggca tcatcctggg cgtgacgcag gcgatcggct
      481 tccgcttcga tccgggctgg ggcatctggt tcggccacat cgtgttcctc gccatgctgg
      541 tgttccgtcc cagcggcctg ttcccgaaga cgcgttgaac catgacgccg cacgcgctga
      601 ccgccaccca gttcgacgtc tcgcgcgcca ccacggcgag ccgcgccggc gcgatgctgg
      661 gcgtgctgct gctggccacc ctggcgacct ttccgttctg ggccgacgcc ggctggatgc
      721 gcgagttcaa cgagatggcg tgttacctga tcttcgccat gatgtggaac ctgctcgccg
      781 gctacggcgg catggtgtcg atcggacagc aggcctatct cgggctgggc ggctacgccc
      841 tgctggtgat gggaaatttc ttcggcgtga acccgttcgt cgcggtgccg atcgcggcgg
      901 tgctgaccat gctggtggcg ctgcccgtgt cccagatcgc cttccggctg cagggcggct
      961 acttcgccat cggcacctgg gtgatcgccg aggtgttccg cctgtcggtg gccaacgtgt
     1021 ccgcggtcgg cggcgggtcg ggcaccagct tcaccgcgct gcgcggcatc gacaaggtca
     1081 cccgccagtc ggtcaccttc tggctctgcc ttgtcggcgc ggtggcgtcg gtggcgctgg
     1141 tgtactggtt cctgcgctcg cgccagggat tggcgctgct cgcgatccgc gacagcgaga
     1201 tcgcctccga gagccagggg gtgaacgtgc gcgccaccaa gctcggggtg tacctggtcg
     1261 cggcgttcgg cgccgggctc gccggcgcgc tgtacttcct cggcaacctg cgcatctcgc
     1321 cggacgcggc gttcacggtc aactggaccg cgttctcgat cttcatcgtc ctgatcggcg
     1381 gcatcggcac catcgaaggg ccgatcatcg gggtgattct gttctacctg ctcagcaagt
     1441 tcttctccga tttcggcacc tggtacctgg tgggcctggg tctgctcgcg atcgtggtga
     1501 ccgtccgctt cccgcgcggg ctgtggggct ggctgtccca gcgcttcgat ctacacttct
     1561 tcccggtgca gcgccgtctg gtgctgcgcg aacccgacaa ggaaacctcc catggctgaa
     1621 gcctttctct gcgacggcgt gcgcacgccg atcgggcgct acggcggggc gctgtcctcg
     1681 atccgcaccg acgacctggc ggcgctgccg atcaaggcgc tgatgcagcg ccacccggac
     1741 ctcgactggc aggcgctgga cgacatcgtg tacggctgcg ccaaccaggc cggcgaggac
     1801 aaccgcaacg tcgcgcgcat ggcgggcctg ctggcgggat tgccggtgga agtgtccggg
     1861 tgcaccgtca accgcctatg cggatccggc atggacgcga tcaacatcgc cgcgcgcgcg
     1921 atcaagtccg gcgaggccga gctgatggtc gcgggcgggg tggaatcgat gacgcgcgcg
     1981 ccgttcgtga tgagcaaggc ggacagcgcc ttctcgcgca ccgcgcaggt attcgacacc
     2041 accatcggct ggcgcttcgt caacaagctg atgaaggaga agcacggcgt cgactcgatg
     2101 ccggagacgg ccgacaacgt ggccgccgac ttcaagatct cgcgcgagga tcaggacaag
     2161 ttcgcgctgc gcagccagca gcgctggcac cgcgccaagg aggccggcaa gttcaaggag
     2221 gagatcgtgc cggtggcggt cccgcagcgc aaaggcgatc cggtgatgtt cgacacggac
     2281 gagcacccgc gcccggacac gaccttggag gcgctggcca agctcaaggg gatcaatgcg
     2341 ccggagctgt cggtgacggc ggggaacgct tcgggcgtga acgacggcgc gtgcgctctg
     2401 atcgtcgcca gcgaaactgc ggtcaagcgg cacggcctgc gccccatcgc gcgcgtggcc
     2461 ggcaccgcga ccgcgggcgt gccgccgcgc atcatgggct tcggccccgc gcccgcatcg
     2521 aggaagctgc tcgcacgtct gggcctcgag gtgcaggata tgaccgtgct ggaactcaac
     2581 gaggccttcg ccagccaggt gctggcggcg ctgcgcgacc tgggcgtgcc cgacgacgcg
     2641 gaagacgtca atcccaacgg cggcgcgatc gccgtcggcc atccgctggg gatgagcggc
     2701 gcgcgcctgg tgatgaccgc cgcctacgag ctgaggcggc gcaacggacg ctacgccctg
     2761 gccacgatgt gcatcggcgt cggccagggc atcgcgatgg tgatcgaacg cgcctgacgg
     2821 cgcacggagg atgttcggga gcagcgggtg gcgcggcctg acggctgcgc agcggcagat
     2881 cggagt
//
LOCUS       DASPGE010000008         3955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_237299, whole genome shotgun sequence.
ACCESSION   DASPGE010000008 DASPGE010000000
VERSION     DASPGE010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237299"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VD791_00245"
     CDS             complement(<1..471)
                     /locus_tag="VD791_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06433.1"
                     /translation="MTPTALHVAAAGLGTLLPALVLAAVAGGALAAEHDPLAGAPEAV
                     VDLATPAGIALMQAQWRYSDTRLTQIAFRAPGSDGQPTGPDSQTYDFLPKAGARDYDD
                     SGWEQIDPGTLSRPRGHGHLSFNWYRVRLTVPPRVGELDPTGSTLVFETQVDDYA"
     gene            complement(468..959)
                     /locus_tag="VD791_00250"
     CDS             complement(468..959)
                     /locus_tag="VD791_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HXV06434.1"
                     /translation="MKTKLSLAALLLVSAASAQAQTIDAKMLTLEGARTIIAGAAAEA
                     RRLDAPGGAITVVDEGGHILALERWDRTFPAASQISIGKARTAALFRKETKAFEDAIN
                     KGRTAMTALPDSVLTPLQGGVPIVVGGRVIGAVGVSGAASAQQDEELAVAGIKALTEG
                     AMR"
     gene            complement(1071..1370)
                     /locus_tag="VD791_00255"
     CDS             complement(1071..1370)
                     /locus_tag="VD791_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HXV06435.1"
                     /translation="MNPTDRVLRAALAGLFAASALSLGAGALADDKDHSKDEKCAGVI
                     KAGKNDCATSKNQCHGHVKTDRDPEAWIYVPKGTCEKIAGAHVTNVFDPTPGVKQ"
     gene            1695..1955
                     /locus_tag="VD791_00260"
     CDS             1695..1955
                     /locus_tag="VD791_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06436.1"
                     /translation="METSKLAVAAAAAALFLAGCTTQGGGSTMQTADAGTVKVKCYGA
                     NACKGQAECKTAMNECKGHNACKGKGFLSMTEKDCLDRLGRA"
     gene            2024..2893
                     /locus_tag="VD791_00265"
     CDS             2024..2893
                     /locus_tag="VD791_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HXV06437.1"
                     /translation="MPDGMLTPPDREFTLPSAGFGLGLRTAHYEAIAARQPAVDWFEI
                     LTENYLVPGGRPLQWLDRIRADYPLAMHGVSLSVGSTDPLDLDYLRAVKALAQRIDPL
                     WISDHLCWTGVAGKNLHDLLPLPYTEAALRHVVARVQAVQELLGRRILLENVSSYVEF
                     EDAEMTEWEFLAQVARQADCLILLDVNNIYVSACNHGFAADDYLAGIPAERVQQIHLA
                     GHSHCGEYIIDTHDAPVADPVWALYERAVQHLGPVTVMIERDDRIPPLPELLAELERA
                     RALAARAQTGQPG"
     gene            2890..3669
                     /locus_tag="VD791_00270"
     CDS             2890..3669
                     /locus_tag="VD791_00270"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HXV06438.1"
                     /translation="MNLHALQDAFQAYVLHGAGQVRERVAPGRLANAERRLRIYHDAY
                     RLRLIEALASDYETLRAALGEEAFRTAALAYVEANVSQWRNVRWYGARLPDFLRGTAP
                     WSDRVWLSDVARFEWTLTLAFDAADAPSATFAELAALPPEAWAALAFRLHPSVHLLRL
                     SSNAPALRKAVDAGAALPAIETHDPPVDWLIWRDGLGVSFRSLAAAEHCALAAVQRGE
                     SFPAICEGICAFVRADEAPALTAGWLRAWVDDGLIAAAVLP"
     gene            complement(3666..>3955)
                     /locus_tag="VD791_00275"
     CDS             complement(3666..>3955)
                     /locus_tag="VD791_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HXV06439.1"
                     /translation="ASRLVGVRSRAAAAAEVHEMSHAGGVMKMRKVEALDLPANRVVK
                     LEPGGFHVMLFDIGQPLKPGERVALTLIVEQAGKRVEVPVDAEVRPLVEKD"
BASE COUNT          565 a         1359 c         1414 g          617 t
ORIGIN      
        1 cgcgtagtcg tccacctggg tttcgaacac cagcgtggag ccggtgggat cgagctcgcc
       61 cacacgcggc ggcacggtca gccgcacgcg ataccagtta aagctcaagt gaccgtgacc
      121 gcgcggccgg gacagcgtgc cgggatcgat ctgctcccag ccgctgtcgt cgtagtcgcg
      181 cgcgcccgcc ttgggaagaa aatcgtaggt ctggctgtcc ggcccggtgg gctggccatc
      241 gctccccggg gcgcggaaag cgatctgggt aaggcgcgta tcgctgtagc gccattgcgc
      301 ctgcatcagc gcgatgccgg cgggcgtggc gagatccacg accgcctccg gcgcgccggc
      361 aagcgggtcg tgctcggcag ccagcgcacc gccggcgacc gccgccagga cgagcgccgg
      421 taacagggtg ccaaggccgg ccgctgcgac gtgcagagcc gtgggagtca tcgcatcgct
      481 ccctcggtca gcgctttgat gccggcgacg gcgagttcct cgtcctgctg cgcgctggcc
      541 gcgccgctca cgccgaccgc gccgatcacc cggccgccga ccacgatcgg gacaccgccc
      601 tgcagcggcg tgagcacgct gtcgggcagc gccgtcatgg ccgtgcggcc cttgttgatg
      661 gcgtcctcga aagccttggt ctccttgcgg aacagcgcgg cggtgcgggc cttgccgatg
      721 gagatctgcg aggcggctgg gaaagtgcgg tcccagcgtt ccagggccag gatatggccg
      781 ccttcgtcca ccacggtgat cgcgccgccc ggcgcgtcga gccggcgcgc ctcggcggcg
      841 gcgccggcga tgatggtgcg cgcgccttcc agcgtcagca tcttggcgtc gatcgtctgg
      901 gcctgtgcgg aagccgcgga aacgagcagc agcgctgcga gcgaaagctt ggtcttcatc
      961 gggtcactcc agtggacagg ggtcggaaga aaacgccctc gccggccgat ttgagcgcat
     1021 cggctcccgc aagcggggac ggcgaaggcg tggttgcggc ggccgcgcgc ttactgtttc
     1081 acgcccgggg tcgggtcgaa cacattggtg acgtgggcgc cggcgatctt ctcgcaggtg
     1141 cccttgggca cgtagatcca ggcctcgggg tcgcgatcgg tcttgacgtg cccgtggcac
     1201 tggttcttgc tggtggcgca gtcgttcttg ccggccttga tgacgccggc gcacttctcg
     1261 tccttgctgt gatccttgtc gtcggccagt gcgccggcgc cgaggctcaa ggcgctcgcg
     1321 gcgaacaggc cggccagagc ggcacgcagg acgcggtcgg tggggttcat gcttgtctcc
     1381 gattgagagt tgcgggggat ggatacaggc gcagcgcagg cggcgatgcc gcccggcact
     1441 acggccctgt gagcgcgcag tgtgcggcgc actcctcacg ccgcgatcac acgaagatca
     1501 acaacgattc acatcgggta gcccgcgcca cgagccgcgt gcatccaagg ggcggtagcg
     1561 cacgtatcac gcgggcgggt ggcgctgggg gaagcggtcc ggcccggcac tgcctgagcg
     1621 cgccctgcag ccttacccag cggcggcgac acgaaggtcc tcgtccggcc cgcatcttac
     1681 gagggagagg ttcgatggag acttccaagc ttgccgtcgc ggcggcggcc gcggcgctgt
     1741 tcctcgccgg ctgtaccacg cagggcggcg gcagcacgat gcagacggcg gacgccggaa
     1801 cggtgaaggt gaagtgctat ggggccaacg cctgcaaggg ccaggccgaa tgcaagacgg
     1861 cgatgaacga gtgcaagggc cacaacgcct gcaagggcaa ggggttcttg agcatgaccg
     1921 agaaggactg cctggatcgc ctcggacgcg cctgaggcgt ggcgggaagc cggtcgtgcc
     1981 cgccggcttc ccgctctttt ctcgtatgcg ccgagaagca atcttgccgg acggcatgct
     2041 cacgccgccg gaccgggaat tcaccttgcc gagcgcaggc ttcggcctgg ggttgcgcac
     2101 cgctcactac gaagcgatcg ccgcccggca gcccgcggtc gactggttcg agatcctgac
     2161 cgagaactac ttggtgcccg gcggccggcc cctgcaatgg ctggaccgga tccgcgccga
     2221 ctacccgctc gccatgcacg gcgtgtcgct gtcggtcggc agcaccgacc ccctcgacct
     2281 cgactatctg cgcgccgtga aggcgctggc gcagcgcatc gatccgctgt ggatctctga
     2341 tcatctgtgc tggaccggcg tggccggcaa gaacctgcac gacctgctgc cgttgccgta
     2401 cacggaagcg gcgctgcgcc acgtggtcgc gcgcgtgcag gcggtgcagg agctgctggg
     2461 ccggcgcatc ctgctggaaa acgtgtccag ctacgtggag ttcgaggacg ccgagatgac
     2521 cgagtgggag ttcctggcgc aggtggcgcg gcaggccgac tgcctgatcc ttctggacgt
     2581 caacaacatc tatgtcagcg cctgcaacca cggcttcgcg gccgacgact acctggccgg
     2641 catcccggcc gagcgcgtgc agcagatcca cctggcggga cacagtcatt gcggcgagta
     2701 catcatcgac acccatgacg cgcccgtggc cgacccggtg tgggcgctgt acgagcgcgc
     2761 agtccagcac ctgggtccgg tgaccgtcat gatcgagcgc gacgaccgta ttcccccgct
     2821 gccggagctg ctggccgagc tcgagcgggc acgagcgctc gccgcgcgtg cgcagaccgg
     2881 gcagcccgga tgaacctcca cgcgttgcag gacgccttcc aagcgtacgt gctgcacggc
     2941 gcggggcagg tccgcgagcg cgtcgcgccc gggcggctcg ccaacgccga gcggcgcctg
     3001 cgaatctacc acgacgctta tcggctgcgg ctgatcgagg cgctcgccag cgactacgag
     3061 acgttgcgcg cggcgttggg cgaggaagcg ttccggactg cggctcttgc atacgtggaa
     3121 gcgaacgtat cgcagtggcg taacgtgcgc tggtacggcg cgcggctgcc cgatttcctg
     3181 cgcggcaccg cgccgtggag cgaccgcgtc tggctgagcg acgtcgcgcg cttcgagtgg
     3241 acgctgacgc tggccttcga tgccgcggac gcgcccagcg cgaccttcgc cgagctggcc
     3301 gcgctgccgc ccgaggcctg ggcggcgctc gccttccggc tgcacccgtc ggtgcatctg
     3361 ctgcggctgt cgagcaacgc gccggcgttg cgcaaggcgg tggacgcggg cgccgcactt
     3421 cccgcgatcg agacgcacga tcctccggta gactggctga tctggcgcga cggcctcggc
     3481 gtgtcctttc gctcgctggc ggccgcggag cattgcgccc tggctgcggt gcagcgcggc
     3541 gagagcttcc cggcgatctg cgaaggcatc tgcgcgttcg tccgcgccga cgaggcgcct
     3601 gcgctcaccg ccggctggct gcgcgcctgg gtggacgacg ggttgatcgc tgcggcggtg
     3661 ctgccctagt ccttctccac cagcggccgc acctcggcgt cgaccggcac ctccacgcgc
     3721 ttgccggcct gctcgacgat cagggtgagc gccacgcgct cgcccggctt gagcggctgg
     3781 ccgatgtcga acagcatcac gtggaagccg cccggctcga gctttacgac ccggttcgcc
     3841 ggcaggtcga gcgcctcgac cttgcgcatc ttcatcacgc ccccggcgtg gctcatctcg
     3901 tgcacttccg ccgctgcggc agcacggctg cgcacgccca ccaggcgcga ggcgc
//
LOCUS       DASPGE010000009         6253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_3288, whole genome shotgun sequence.
ACCESSION   DASPGE010000009 DASPGE010000000
VERSION     DASPGE010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3288"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VD791_00280"
     CDS             <1..687
                     /locus_tag="VD791_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXV06440.1"
                     /translation="VRQRRGLFEQAEGGTLFLDEIGDMPPAMQVKLLRAIQERKITRV
                     GGERPLAVNIRLACATNRDLRRLVEQGAFREDLYYRINVIHLRVPALRERVEDVLWLA
                     ERFLRERTGAAGTRRLSAAAQSALLRHAWPGNARELKHCIERACILARGPSIEPWDLF
                     QAEPERGGDGARSETLNAYLRACEREFITRALEEHGWRVTETATELGISRKNLWEKMR
                     RLDIDGRRDD"
     gene            919..2757
                     /locus_tag="VD791_00285"
     CDS             919..2757
                     /locus_tag="VD791_00285"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV06441.1"
                     /translation="MLWKRRLIVVAMIGAGLVGALAFDEYQAVRSDRTSLLLSTIGAP
                     FCTPQRSGPQYKTFFRLAMAQTERGGKPARTEVGPFSRAIPNAESLAGADANPVLADD
                     LGTLHYPIATSVPLAQRFFDQGLRLAYAFNHGEALRAFRKARTLDPNCAMCYWGEALV
                     LGPNINAPMDGASVAPAFAAVTKAKALSANASPKHRALIDALAARYSVDPAADRAALD
                     KAYADAMQAVAARYPSDDEIAVQYAESLMDLQPWDYWEGGSKPKGRAAEIVGQLERVL
                     ARSPSHPGAIHYYIHVVEASSDPKRALPHARRLGRVMPGAGHLVHMPFHIFFRAGLFK
                     EAVESNRQAVAADEAFIARSAPVGIYPDAYYPHNVHSLLVSAQMAGDGKTVLAAADKL
                     EAIVSDAAAQNIAWVQPIKAAPFFAHAQFSDAPTILALADPGLDFPYVQAMWHYARAV
                     ALAGTGDLSAAQAEVDAITRLQEYADLSALAAGGVPVNDVLQLAQHVARGRIAQARQD
                     LTAAIGHYQAAVAVEDMLAYSEPPFWYYPTRQSLGAALLLAGDLDRAEQVLRASLQRT
                     PNNGWALFGLMKVYEQRGDERSARSAGKLLSEAWIGSMHDLDLARL"
     gene            2869..3630
                     /locus_tag="VD791_00290"
     CDS             2869..3630
                     /locus_tag="VD791_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXV06442.1"
                     /translation="MLLLHGFTDSWRSFEPVLPHLPESVHAFAVTQRGHGGLHRPSIG
                     YRTRDFAADVVGFLDALGLGRAILVGHSMGATNALRFAIDYPERTLGLALAATFATYR
                     SNPVVMEFWENGVSRLTDPIDPAFARDFQQGTLARPLPARFLDTVVRESLKVPAHVWR
                     TAFQGFLEDDFVDELAEIEAPTLVLWGTQDVLCPRADQETLLAAIAGARLAVYEGAGH
                     ALHWEEPERFAADLAAFARTVPDLPRAYAAPLAVL"
     gene            3755..4513
                     /locus_tag="VD791_00295"
     CDS             3755..4513
                     /locus_tag="VD791_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HXV06443.1"
                     /translation="MEWLFTPEGLIALATLTALELVLGIDNVIFISILAGKLPPGQRD
                     NARRLGLLVAAVSRLALLFSIAWIIGLTDPLFALLGQEISWRDIILITGGLFLIYKGT
                     SELHQRLEGETGVKGAAVRATFAGVLLQVFILDMVFALDSIITAVGMVDDVGIMAAAI
                     ILSVALMLLAAKYIHGFVERHPTIKVLALSFLLMIGMVLIADGLEVKVPKGYVYFAMG
                     FSVFVELLNMRMRKVQREAPVRLHDAYAQDDRKA"
     gene            complement(4582..>6253)
                     /locus_tag="VD791_00300"
     CDS             complement(4582..>6253)
                     /locus_tag="VD791_00300"
                     /inference="COORDINATES: protein motif:HMM:NF009310.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit D"
                     /protein_id="HXV06444.1"
                     /translation="SVHPGLLLIAAGIGLPLLPRALRPGLILLAPLAALALTWQLPDG
                     ELWRVPLLDYELTLLRSDRLARLFAIVFALMAFGGGLYALTQRSRVEMPAAFTYAGSA
                     IGATLAGDLITLFVFWELMALASTLVLWSAGTRAYRPGLRYLAMHLLGGVLLMAGIAG
                     HVTESGSIAFDAMTGGSPAQWLILAGFLINAAAPPLSAWLPDAYPAASWSGTVFLSAF
                     TTKTAVYALLRGFPGCELLLYLGVFMIFYGIVYALLENDMRRILAYSIVNQVGFMVAG
                     AGLGTELAINGAAAHAFAHIIYKALLLMSAGSVLYMTGRSKCNELGGLFRSMPLTTAC
                     GIVGALSISAFPLTSGFVSKSMISQAAADAHLTSLWLLLAAASAGVFLHAGIKFPWFV
                     FFQKDSGLRPPDPPFNMRLAMLLFAVLCLGLGVYPAPLYALLPYPVDYVPYTPWHVIA
                     QLQLLLFSGLAFFVMLPWLKRTPTITLDVDWLYRAAGARLASSVLEASSATYRRLAVH
                     LQRAMGAALVRIYRHHGPSGVLARTWPTGSMVLWVAILLASYLLLYYL"
BASE COUNT         1021 a         2090 c         2190 g          952 t
ORIGIN      
        1 gtgcgccagc gccgcggcct gttcgagcag gccgagggcg gcacgctgtt cctggacgag
       61 atcggcgaca tgccccccgc catgcaggtc aagctgctgc gcgccatcca ggagcgcaag
      121 atcacgcgcg tcggagggga gcggccgctg gcggtgaaca tccggctggc ctgcgccacc
      181 aaccgcgact tgcggcgcct ggtcgagcag ggtgccttcc gcgaggacct ctactatcgc
      241 atcaacgtca tccacctgcg cgtgccggcg ctgcgcgagc gcgtcgagga cgtgttgtgg
      301 ctggccgagc gcttcctgcg cgagcgcacc ggcgccgccg gtacgcgccg cctgtccgcc
      361 gccgcccagt cggcgctgct gcgacacgcc tggcccggca atgcgcgcga gctcaagcac
      421 tgcatcgagc gcgcttgcat ccttgcgcgc ggcccgtcga tcgagccctg ggacctgttt
      481 caggccgagc ccgagcgcgg cggggacggg gcgcggtcgg agacgctgaa cgcctacctg
      541 cgcgcgtgcg agcgcgaatt cattacacgt gcgctggagg aacacggctg gcgcgtcacc
      601 gagacggcga cggaactggg gatcagccgc aagaacttgt gggagaagat gcgcaggctg
      661 gatatcgacg gacggcgcga cgactgatcg gccgtcggtc gcgtcgtacc gttacaaaca
      721 cgatacaaac cgtttccgaa cggacatcgc cgggttaccg ccggcctcca tactccgctc
      781 ccgccgcccc aagcgctttg tacgacggcc gcttatacga cggccgcttg tgcgacgtcc
      841 ggcttgcgtt gcatccgagg aatcgccgcg ccgccggtgc gcgctgtcgg ggcttcctgt
      901 tttcgaagag gtatcgatat gttgtggaaa cgcagactta tcgttgtcgc catgatcggc
      961 gccggcctgg tgggcgcgct ggcattcgac gaataccagg cggtccgcag cgaccgcacg
     1021 tccctgctgc tgagcacgat cggtgcgccg ttctgcacac cgcagcgcag cggcccgcag
     1081 tacaagacgt tcttccggct ggcgatggcg cagacggagc gcggcggcaa gccggcgagg
     1141 accgaagtcg gaccgttcag cagagcgatc ccgaacgccg aatcgctggc cggggccgac
     1201 gccaacccgg tgctggccga cgacctcggg acgttgcact atccgatcgc cacctcggtg
     1261 ccgctggcgc agcgcttctt cgaccagggc ttgcgcctgg cgtacgcgtt caaccacggc
     1321 gaggcgctgc gcgcgttccg caaggcgcgc accctcgatc cgaactgcgc gatgtgctac
     1381 tggggcgagg cgctggtgct cggacccaac atcaacgcgc cgatggacgg cgcctccgtg
     1441 gcgcccgcat tcgcggcggt gaccaaggcg aaggccttgt cggcgaacgc gagcccgaag
     1501 catcgcgcct tgatcgacgc gctggcagcc cgctactcgg tggaccccgc ggccgaccgc
     1561 gcggcgctcg acaaggcgta tgcagatgcg atgcaggcgg ttgcggcgcg ctacccgtcg
     1621 gacgacgaga tcgcggtgca gtacgccgag tcgctgatgg acctgcagcc ctgggactac
     1681 tgggaagggg gcagcaaacc caaagggcgc gcggcggaga tcgtcggcca gctcgagcgc
     1741 gtgctggcga gatcgcccag tcatccgggc gcgatccact actacatcca tgtggtggag
     1801 gcctccagcg accccaagcg cgccttgccc cacgcgcggc gccttggccg ggtgatgcct
     1861 ggcgccgggc acctggtgca catgccgttt cacatcttct tccgtgccgg cttgttcaaa
     1921 gaggcggtgg agtccaaccg gcaggccgtg gccgccgacg aggccttcat cgcgcggtcg
     1981 gcgccggtcg gcatctatcc cgatgcgtat tacccgcaca acgtccactc gctgctggta
     2041 tcggcgcaga tggccgggga cggcaagacg gtcctcgccg ccgccgacaa gctggaggcc
     2101 atcgtgtcgg atgccgcggc ccagaacatc gcctgggtgc agccgatcaa ggcagcgccg
     2161 tttttcgccc atgcgcagtt cagcgacgcc ccgacgattc tcgcgctggc cgatccgggg
     2221 ctcgatttcc cgtacgtcca ggcgatgtgg cattacgcgc gcgcggtggc gttggccggc
     2281 accggggact tgtccgcggc gcaggcggag gtggacgcca tcacgcgact gcaggagtac
     2341 gccgaccttt ccgctctggc cgcgggcggc gtcccggtga acgacgtgct gcagctcgcg
     2401 cagcacgttg cccgcggacg catcgcgcaa gcacggcagg acttgacggc cgccatcggc
     2461 cattaccagg cggcggtggc ggtggaggac atgctggctt actcggagcc gcccttttgg
     2521 tactacccga cacgccaatc gctcggcgcc gccttgctgc tcgcgggcga cctggaccgg
     2581 gccgagcagg tgctgcgcgc gagcctgcag cgcacgccca acaacggctg ggcgctgttc
     2641 ggcttgatga aggtctacga gcagcgtggc gacgagcgca gcgcccgctc cgccgggaag
     2701 ctgctgagcg aggcgtggat cggcagcatg cacgacctgg atctggcgcg gctctaaacc
     2761 gaccttctca tggagaccca gcctgtgaca ccgtcggtcc gatgcgtgga actgcctacc
     2821 ggggtcgagc tcgcctacgt cgagcagggt gatcccgccg gcgtacccat gttgctgctg
     2881 cacgggttca ccgattcgtg gcgctccttc gagccggtct tgccgcatct tcccgagtcc
     2941 gtgcacgcgt tcgccgtgac acaaaggggg cacggcggcc tgcatcgccc gagcatcggt
     3001 taccggacgc gggatttcgc tgcagacgtg gtcggcttcc tggacgcgct cgggctcggg
     3061 cgggcgatcc tcgtcggcca ttcgatgggc gccaccaatg ccttgcgctt cgcgatcgac
     3121 tatcccgagc gtacgctcgg cctggcgctg gcggccacct tcgccaccta ccgcagcaac
     3181 cccgtcgtca tggagttctg ggagaacggg gtatcgcggc tcaccgatcc gatcgacccg
     3241 gcgttcgcgc gggatttcca gcaaggcacc ttggcgcgcc cgctgccggc gcggttcctc
     3301 gacaccgtcg tgcgggagag cctgaaagtg cccgcgcacg tgtggcgcac ggcgtttcag
     3361 ggctttctcg aggacgattt cgtggatgag ctcgccgaga tcgaagcgcc cacgctcgtc
     3421 ctgtggggca cgcaggacgt gttgtgcccg cgcgccgatc aggaaacgct gctcgcggcc
     3481 atagccgggg ctcggctcgc ggtgtacgag ggggccgggc atgcgctgca ttgggaggag
     3541 ccggagcgct ttgcggctga tctcgccgcg ttcgcccgga cagtcccaga cttgccccga
     3601 gcgtacgccg ccccgttggc tgtgctctga cgccgcgccg atcgtcgtag actcccgtgc
     3661 ccgggcggcc acgctgcccg ggcgcgcttc gcgcgcctgc attcttgcgt accattgtct
     3721 tccgacgtcc aactagaccg aaggaagacc gccgatggag tggctgttca cgccggaagg
     3781 actgattgcc ctcgcgacgc tcaccgcgct cgagctggtg ctgggtatcg acaacgttat
     3841 cttcatttcg atcctggccg gcaagctgcc gccggggcag cgcgacaacg cgcgccggct
     3901 cggcctgctg gtcgccgcgg tgagccggct cgcgctgctg ttctcgatcg cctggatcat
     3961 cggtctcacc gacccgctgt tcgccctgct cgggcaggag atctcgtggc gcgacatcat
     4021 cctgatcacg ggcggattgt tcttgatcta caagggcacc agcgagctgc accagcgcct
     4081 ggagggcgaa accggcgtga aaggggcggc ggtgcgggcc accttcgccg gtgtgctgct
     4141 gcaggtgttc atcctcgaca tggtgttcgc gctggactcg atcatcaccg cggtgggcat
     4201 ggtcgacgac gtcgggatca tggcggccgc gatcatcctg tcggtcgcgc tgatgctgct
     4261 cgcggcgaag tacatccacg gattcgtcga gcggcatccc accatcaagg tgctggcgct
     4321 gtccttcctg ctcatgatcg gcatggtgct gatcgccgat ggtctcgagg tcaaggtgcc
     4381 gaagggctac gtgtacttcg cgatgggctt ctcggtgttc gtcgagctgc tgaacatgcg
     4441 catgcgcaag gtccagcgcg aagcgccggt gcgcctgcac gacgcgtacg cgcaggacga
     4501 ccggaaggcc tgagccggcc cagaaatgcc gggctcagag gtagtagcgc agcaggtgag
     4561 gcaggccccg aggtgccagg ctcacagata gtagagcagc aggtaggagg ccagcagaat
     4621 cgccacccac agcaccatgc tgcccgtggg ccaggtgcgc gcgagcacgc ccgacgggcc
     4681 gtggtggcgg tagatgcgga cgagtgcggc gcccatcgcg cgctgcaagt gcacagcgag
     4741 acggcgataa gtggcgctgg aggcctccag gacgctgctc gccaggcgcg cgccggccgc
     4801 ccggtacagc caatccacgt cgagcgtgat cgtcggcgtg cgcttgagcc agggcagcat
     4861 gacgaagaag gccagccccg agaacaatag cagttgcagc tgcgcgatca cgtgccaggg
     4921 cgtgtagggc acgtagtcca ccgggtaggg cagcagcgcg tagagcggcg ccggataaac
     4981 gcccagccca aggcacagca ccgcgaacag tagcatggcc aggcgcatgt tgaacggcgg
     5041 gtcgggcggg cgcagtcccg aatccttctg gaagaacacg aaccagggga acttgatgcc
     5101 ggcgtgcagg aacacgccgg cggatgccgc ggcgagcagc agccacagcg aggtcaggtg
     5161 agcatcggcg gcggcctgcg atatcatcga tttagacacg aacccggagg tgagcgggaa
     5221 cgccgagatc gagagcgctc cgacgatgcc gcacgccgtg gtcagcggca tgctgcggaa
     5281 caggccgccc agctcgttgc acttgctgcg cccggtcatg tacagcaccg aaccggcgga
     5341 catcagcagc agcgccttgt agatgatgtg cgcgaatgcg tgcgcggccg cgccgttgat
     5401 cgccagctcc gtgcccaggc ccgcgcccgc caccatgaac ccgacctggt tgacgatgct
     5461 gtaggccagg atgcggcgca tgtcgttctc cagcagggcg tagacgatgc cgtagaagat
     5521 catgaacacg cccagataga gcaacagctc gcagcccggg aaaccgcgca gcagcgcgta
     5581 gaccgcggtc ttggtggtga aggcggagag gaacacggtg ccggaccagg acgccgcggg
     5641 ataggcgtcg ggcaaccagg ccgacagcgg tggcgccgcg gcgttgatca ggaatccggc
     5701 gagtatcaac cactgcgccg gggagccgcc ggtcatggcg tcgaaggcga tcgagcccga
     5761 ctcggtgaca tggccggcga tgcctgccat gagcagcacg ccgcccagca agtgcatggc
     5821 cagatagcgc aggccgggtc ggtaagcgcg cgtgccggcg ctccatagca ccagcgtcga
     5881 ggcgagcgcc atcagctccc agaacacgaa cagggtgatg aggtcgccgg cgagcgttgc
     5941 gccgatcgcg ctccccgcgt aggtgaacgc ggcgggcatc tcgacgcggc tgcgctgcgt
     6001 gagggcgtaa agcccgccgc cgaaggccat cagcgcgaac acgatggcga acagccgcgc
     6061 cagccggtcg ctgcgcagta gcgtcagctc gtagtcgagc agcggcacgc gccacagctc
     6121 gccatcgggg agctgccagg tcagcgccag cgccgccagc ggcgccagca ggatcaggcc
     6181 ggggcgcagc gcgcgcggca gcagcggcag cccgatcccg gcggcgatca ggagcaggcc
     6241 cgggtgcacg cta
//
LOCUS       DASPGE010000010         3287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_238273, whole genome shotgun sequence.
ACCESSION   DASPGE010000010 DASPGE010000000
VERSION     DASPGE010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238273"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VD791_00305"
     CDS             <1..1009
                     /locus_tag="VD791_00305"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HXV06445.1"
                     /translation="CPPDTDPPLDEPGLVEMLKFRARSLKQARVYPLGALTQGLKGER
                     LTEMAELRDAGCIGYGQADAPITDRQVLSRALQYATTFGFCVWLRAQDPSLAHGGVAH
                     DGQVSTRLGLPGIPVSAETIALSTILILAQETGARIHLCRLSSAAGLELVRRARQEGM
                     PVTCDVAAAHVHLSEIDIGFFDSNCNLVPPLRSLRDRVALRQGLAEGSVDAICSDHTP
                     VDDDAKQRPFAEAEPGATGLELLLPLTLKWAQEARIPLPVALRKVTSEPLRVLGLAGG
                     RLRPGEAADLCLFDAQAHWKVEPAALFSQGKNTPFLGYELPGRVRYTLVGGSVVFEAK
                     A"
     gene            1006..3051
                     /locus_tag="VD791_00310"
     CDS             1006..3051
                     /locus_tag="VD791_00310"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HXV06446.1"
                     /translation="MNPLLDFSGLPRFGEIQPEHVTPAVDQLLAENRALVERVATAAE
                     PPTWDGFVAPIDDANERLSRAWGQVGHLNAVMNSPPLREVYNANLPRVTQYYTELAQD
                     ERLYAKFKALRAAGEYGRLGAAQRRIIDNELRDFWLSGAELAPERKARFKAIRARLDE
                     LSSRFNDNVLDATNAFGHIVTDAAQLAGIPADALEAARAAAQADGKEGWKFTLHMPSY
                     LPVMQYADHRALRSHMYQAYVTRASELGKPQCDDTQWDNTPLIAEILKLRKEAAQLLD
                     FESYAAFSLTPKMADSPIQVLDFLDDLAARVKPFAEREFEELTRFAATELGLPRLEAF
                     DVGWAAEKLRLARYAFSEQEVKRYFPEDRVLPGMFRLVERLYGIRIAPAQAPVWHPDV
                     RFFDIRRPDGELIGQFYLDLYARPSKRGGAWMDDAITRKRRADRIQTPVAYLNCNFSG
                     PVGGRPALFTHSEVTTLFHEFGHGLHHLLTRVEHRGVSGIAGVEWDAVELPSQFMENF
                     CWEWDVLQGMARHVDSGAPLPRELYDKMLAAKNFHSGMMFVRQLEFALFDMHLHHDFD
                     PDGPMTALQLLDQVRRRVAVVIPPPYNRFPHSFSHIFAGGYAAGYYSYKWAEVLSADA
                     YSLFEEQGVLNPEVGERFWREILGVGGSRPALESFVAFRGREPRIEALLRHSGMAVA"
     gene            3092..>3287
                     /locus_tag="VD791_00315"
     CDS             3092..>3287
                     /locus_tag="VD791_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HXV06447.1"
                     /translation="MKRIALLFGLAVAADIGTARAAEMYRWVDEAGRVHYSDTLPPPE
                     ARSRERRRLGDKPTPSTLPYA"
BASE COUNT          512 a         1156 c         1119 g          500 t
ORIGIN      
        1 gtgcccgccc gacaccgacc cgcctctgga cgagcccggg ctggtggaga tgctcaagtt
       61 ccgcgcgcgc agcctcaagc aggcgcgcgt gtacccgctg ggcgcgctga cccaggggct
      121 gaagggcgag cgcctgaccg agatggccga actgcgcgac gccggctgca tcggttacgg
      181 ccaggcggac gcgccgatca ccgaccggca ggtgctttcg cgcgcgctgc agtacgccac
      241 cacgttcggc ttctgcgtat ggctgcgcgc ccaggatccc agcctcgcgc acggtggcgt
      301 ggcgcacgac ggccaggtat ccacgcgcct gggactgcca ggcatcccgg tgtcggccga
      361 gaccatcgcg ctgtccacca tcctgatcct ggctcaggaa accggcgccc gcatccatct
      421 gtgccggctg tccagcgccg cgggcctcga gctggtgcgc cgcgccaggc aggagggcat
      481 gccggtgacc tgcgacgtag cggccgcgca cgtgcacctg agcgagatcg acatcgggtt
      541 cttcgattcg aactgcaacc tggttccgcc gctgcgcagc ctgcgcgacc gcgttgccct
      601 gcgccagggg ctggccgagg gcagcgtcga cgccatctgc tccgaccaca cgccggtgga
      661 cgacgacgcc aaacagcggc ctttcgccga ggccgagccc ggcgccaccg gcctggaact
      721 gctcctgccg ctgacgctca agtgggcgca agaggcgcgc atcccgctgc ccgtggcgct
      781 gcgcaaggtg accagcgagc cgctgcgcgt cctcggcctt gccggcggac gactgcgtcc
      841 cggcgaagcg gcggatctgt gcctgttcga tgcgcaggca cactggaagg tcgagccggc
      901 ggcgctcttc agtcagggca agaacacgcc gttcctgggc tacgagctgc cgggacgcgt
      961 gcgctacacg ctggtcggcg ggtcggtggt cttcgaagcc aaagcatgaa tccgttactc
     1021 gatttctcgg gcctgccgcg cttcggcgag atccagccgg agcacgtcac gcccgccgtg
     1081 gaccagctgc tcgccgagaa ccgggcgctg gtcgaacgcg tcgcgaccgc ggccgagccg
     1141 cccacctggg acgggttcgt ggcgccgatc gacgatgcca acgagcgcct gtcgcgcgct
     1201 tggggacagg tcgggcacct caacgcggtg atgaacagtc cgccgctgcg cgaggtgtac
     1261 aacgccaact tgccgcgcgt cacgcagtac tacacggagc tcgcccagga cgagcggctg
     1321 tacgcgaagt tcaaggcgct gcgcgccgcc ggcgaatacg gccgcctcgg cgccgcgcag
     1381 cgcaggatca tcgacaacga gctgcgcgac ttctggttga gcggcgccga gctggcgccg
     1441 gaacgcaagg cgcgcttcaa ggcgatccgc gcgcggttgg acgagctgtc ctcgcgcttc
     1501 aacgacaacg tgctggacgc caccaacgcg ttcggccaca tcgtgaccga tgccgcgcag
     1561 ctggccggca tcccggccga cgcgctggaa gcggcgcgcg ccgccgcgca agccgacggc
     1621 aaggagggct ggaagttcac cctgcacatg ccctcgtacc tgccggtgat gcagtacgcc
     1681 gatcaccgcg cgctgcgcag ccacatgtac caggcctacg tcacgcgcgc ctccgagctg
     1741 ggcaaacccc agtgcgatga cacccagtgg gacaacacgc cgctgatcgc cgagatcctg
     1801 aagctgcgca aggaggccgc gcagctgctc gacttcgaga gctatgcggc gttttcgctc
     1861 acccccaaga tggccgactc gccgatccag gtgctcgact tcctggacga tctcgcggcg
     1921 cgggtgaagc ccttcgccga gcgcgagttc gaggagctga cccggttcgc cgccaccgag
     1981 ctgggcctac cccggctcga ggcgttcgac gtcggctggg ccgccgagaa gctgcggctt
     2041 gcgcgctacg cgttctcgga gcaggaggtc aagcgctatt tccccgagga tcgcgtgctc
     2101 cctggcatgt tccgcctggt ggagaggctg tacggcatcc gcatcgcccc ggcgcaagcg
     2161 ccggtgtggc acccggacgt gcgcttcttc gacatccgta ggccggacgg cgagctgatc
     2221 ggacagttct atctcgacct ctacgcccgg ccatccaagc gcggcggagc ctggatggac
     2281 gacgccatca cccgcaagcg ccgcgcggac cgcatccaga cgccggtggc ctacctcaac
     2341 tgcaatttct ccggccccgt gggcggccgg ccggcgctgt tcacccacag cgaggtcacg
     2401 acgctgttcc acgagttcgg ccacggcctg caccacctgc tcacgcgcgt ggagcatcgc
     2461 ggtgtgtccg gcatcgccgg cgtggaatgg gatgcagtgg agctgcccag ccagttcatg
     2521 gagaatttct gctgggaatg ggacgtgctg cagggcatgg cgcggcacgt ggacagcgga
     2581 gcgccgctgc cgcgcgagct gtacgacaag atgctcgcgg cgaagaactt ccacagcggc
     2641 atgatgttcg tgcgccagct cgagttcgcg ctgttcgaca tgcacctgca ccacgacttc
     2701 gacccggacg gtccgatgac cgcgctgcag ctgctggacc aggtgcgccg ccgcgtggcg
     2761 gtggtgatcc cgccgccgta caaccgcttc ccgcacagct tctcgcacat cttcgcgggc
     2821 ggctatgcgg ccggttacta cagctacaaa tgggccgaag tgctctccgc cgacgcctac
     2881 agcctgttcg aggagcaggg ggtgctcaac cccgaggtcg gcgagcgctt ctggcgcgag
     2941 atcctggggg tggggggcag ccgcccggcc ctggagtcct tcgtcgcctt ccgcggccgg
     3001 gagccccgga tcgaggcgtt gctgcgccac agcggcatgg ctgttgcttg agttgttccg
     3061 gaagggaacc tgccccatcg gggagctcac catgaaacgg atcgcgctgc tgttcggttt
     3121 ggccgtcgcg gcggatatcg ggaccgctcg ggcggccgag atgtatcgct gggtcgatga
     3181 agccggccgg gtccactact ccgacacatt gcctccaccc gaggcgcgca gccgcgagcg
     3241 cagacggttg ggcgacaaac ccaccccctc gacgttaccc tacgctt
//
LOCUS       DASPGE010000011         7799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_940369, whole genome shotgun sequence.
ACCESSION   DASPGE010000011 DASPGE010000000
VERSION     DASPGE010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940369"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..774)
                     /locus_tag="VD791_00320"
     CDS             complement(241..774)
                     /locus_tag="VD791_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HXV06448.1"
                     /translation="MSSASLRHAPLAGFLLALVLVILASNILVQTPLNDWLTWGAITY
                     PFSFLITDLANRYYGLRFARRLVYAGFVIALVLSAYYATPRIALASGTAFLVAELADV
                     QIFDRLRRRAWWQPPLVSTLVGSVLDTAVFFTLAFYGTGMPWITLGLGDLGVKVALAV
                     LTLLPFRAALALFRPAV"
     gene            complement(793..1149)
                     /locus_tag="VD791_00325"
     CDS             complement(793..1149)
                     /locus_tag="VD791_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXV06449.1"
                     /translation="MSIIDLLPSETPLKSIPAGEVVFAQGDAGDSMYVVIEGEVSVLI
                     DGKVVETVRAGGVVGELALIDSQPRSATAISRTRCLVSPVDERRFMDLIAREPEFALQ
                     VMRVMAKRLRRMDSER"
     gene            1411..2427
                     /locus_tag="VD791_00330"
     CDS             1411..2427
                     /locus_tag="VD791_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HXV06450.1"
                     /translation="MIAHRGASGYLPEHTLAAYYLAIQQGADFVEPDLVMTRDGVLVA
                     RHENEIGATTDVSAHPEFVARKTRKTIDGEQVEGWFTEDFTLAELKTLRARERLPRLR
                     PGNTRFDGMLEVATLDEVLALVQAMDWQRRMLAQQDGRTAPPPIGVYPETKHPSYFAG
                     LGLPMEAALVRTLQRWGYAGRDAAAFIQSFETANLKALRRMSELRLVQLVDSGGRPHD
                     FVVAGDPRPGSDLLTPRGLAEIAGYADAVGVNKSLIIPRNADGSLAAPTTLVADAHAA
                     GLLVHAWTFRAENEFLPREFLSGNDAAVLGDMVGEVRRFLDAGVDGVFTDHPDLGIKA
                     VDRR"
     gene            complement(2481..3491)
                     /locus_tag="VD791_00335"
     CDS             complement(2481..3491)
                     /locus_tag="VD791_00335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXV06451.1"
                     /translation="MRILIVTDAWEPQVNGVVRTLKSTCRELQAMGHEVGLITPLEFR
                     TIPCPTYPDIRLSLFPRAKVARRIRAFRPNALHIATEGPLGMAARACARRIGMPFTTA
                     YHTRFPEYVHARFAVPLSWTYAFLRRFHAASSAVMAPTRMVVRDLQQWGFKNVVQWTR
                     GVDLEVFSFRRCQRLKSTPPIFLYVGRVAVEKNVEAFLELDLPGSKWVVGDGPALPAI
                     RIKYPQAHYLGVLDQSEVAELYASADVFVFPSRTDTFGLVLLEAMACGLPVAAYPVTG
                     PLDVVGDSGGGVLDEDLRVACLAALNIERSAARAVAERFSWRAATEQFERHLHPVDLQ
                     PG"
     gene            3710..4972
                     /locus_tag="VD791_00340"
     CDS             3710..4972
                     /locus_tag="VD791_00340"
                     /inference="COORDINATES: protein motif:HMM:NF023327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtaA family protein"
                     /protein_id="HXV06452.1"
                     /translation="MRAGRGKARATSRGTGLNQVARKLIDSLDQKVFDTLSGRSLVYN
                     TCWEDPAVDRQALCLSASDRLLVITSAGCNTLDYALQGPARIDAVDANPRQTALLELK
                     LAGIRRLTFDDFFRVFGDGYHPAFRDLYRSALRGELSNFARSYWDRNGDWFCQKHGRD
                     TFYYFGLSGLMARAFRAYLRVRPRLRAAIEAVFDAGSLPAQREIYDSQIGPLLWSRAM
                     NWTLSRQLTMSLLGVPHTQRKEVEQQHPGGVAGFIRQAIEYVARELPLSSNYFWSLYL
                     RGGYTRECCPEYLKRDNFAALKGGLVDCIHPHTATVTEFLDRGHMPLSRFVLLDHMDW
                     MSGHRPQALVEEWEAILRRACSGARAIFRSAHARPSYLEAVHVERDGRTRPLRDLLRF
                     HRALARRLSLQDRVHTYAGYHIADIETD"
     gene            4972..5718
                     /locus_tag="VD791_00345"
     CDS             4972..5718
                     /locus_tag="VD791_00345"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV06453.1"
                     /translation="MLSTADAHVLWALIRGRSRAATHAQRLQDFYADQADRYDAFRER
                     LLQGRRELIERLPVGPGARVTELGAGTGRNVEFFGERLARLRSIVLVDLCPALIEQAL
                     RRRHRLAASGVSNISVVHADATTYRPCGPRDCVYFSYALTMIPDWAGAIDNAIAMLRP
                     GGVLGVVDFHLPDRVPVLDAFWRRWFAHDGVWLSDRHLPYLRRRLRPLFAREGRARLP
                     LLPGLRAPYYLYIGQKAAAPEMGGEGETGD"
     gene            5723..6559
                     /locus_tag="VD791_00350"
     CDS             5723..6559
                     /locus_tag="VD791_00350"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HXV06454.1"
                     /translation="MAEVRSIFLSDIHLGTRACQADRLLDFLRHFESRYLFLVGDILD
                     FWAMGQRGVYWSASMNTFVQKMLKRARHGTEVIFIPGNHDEALRDYLDSTFGNIGLHL
                     EYEHRSADGKRYLVLHGDQFDVVTKYHRWLAVLGDHGYALLVRLNVLLSWVRRQLGIG
                     GYWSLAGYAKRKVKTAVSFICDFEEAVARHVKERGLDGAICGHIHAAAIKQVDGVTYI
                     NCGDWVDSCTAIVEYLDGRMELIDWAAAPAAAGSSETSQQRSPALAAHGRAGSAAEHE
                     TV"
     gene            complement(6572..7573)
                     /locus_tag="VD791_00355"
     CDS             complement(6572..7573)
                     /locus_tag="VD791_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV06455.1"
                     /translation="MRTTALPGAWRLLGMAVLLWATHAPVVLAQSPPKLIFLTNWYAQ
                     AEHGGFYQAVAEGTYRKHGLDVDVKMGGPQVNVVQLLLAGQADLVMGYDLQTIKLIEQ
                     GLPVVTVGATFQKDPAVLIAHPDVKTMADLATRTLLIGQASETTFWPWLRAKYGFTEA
                     QKRPYAFSVQQFLVDRNVAQQGYATSEPYSIEKAGVKPVVFLLADQGYPPYAQTIVVT
                     RKTAQDKRDLVRRFLQATAEGWKSYLNQPAPGNALIKKANPQMEDELMAFGLRKMREF
                     GLVTGGDARTQGILTMTDARWKRTFDFMAGAGLAPPDLDYRQAYTLEFVKAVKVLPQ"
BASE COUNT         1243 a         2674 c         2666 g         1216 t
ORIGIN      
        1 gacccgcggc gcgcccgagc cggctacggc agccaccgtc gtacggcagg cggccgcccc
       61 cgctctgccc cgcggtaggc cccttatcac cctccggccg atcgacccgg cgccgggcct
      121 gcaggtccgg cgcggaccga ccggaagcca gcatacatca gccgtagacg cgatggtggg
      181 gtgcgcaggg cgcccctctc gggtgcccgt ccaacctgtg gccggtccgg cccggaacgc
      241 tcagaccgcg ggccggaaca gcgcgagcgc ggcgcggaac ggcagcagcg tgagcaccgc
      301 cagggcgacc ttcacgccga gatcccccag ccccagggtt atccacggca tgccggtacc
      361 gtagaaggcc agcgtgaaga acaccgcggt atcgaggacg gagccaacca gcgtggaaac
      421 cagcggcggc tgccaccagg cgcgccggcg caggcggtcg aagatctgca cgtccgccag
      481 ctcggccacc aggaaggcgg tgcccgaggc gagcgcgatg cgcggggtgg cgtagtaggc
      541 cgacaggacc agcgcgatca cgaagcccgc gtacaccagc cggcgcgcga agcgcagtcc
      601 gtagtaacgg ttggcgaggt cggtgatcag gaagctgaag ggataggtga tcgctcccca
      661 ggtgagccag tcgttgagcg gcgtctgcac caggatgttg gacgccagga tcaccagcac
      721 cagcgccagc aggaagccgg cgaggggcgc gtggcgcagc gacgcggaac tcatcgcttt
      781 gaaatatccg ggctagcgct cggagtccat ccgccgcagc cgcttggcca tcacccgcat
      841 cacctgcagg gcgaactccg gctcgcgcgc gatcagatcc atgaagcgcc gttcgtcgac
      901 cgggctgacc aggcagcggg tgcgcgagat ggcggtggcg ctgcgcggct gcgagtcgat
      961 cagcgccagc tcgccgacca cgccgccggc gcgcaccgtc tccaccacct tgccgtcgat
     1021 cagcaccgag acttcgcctt cgatgaccac gtacatagaa tcgcccgcat cgccctgcgc
     1081 gaacaccacc tcgcccgccg ggatagactt gagcggggtc tcgctcggca gcagatcgat
     1141 gatcgacatg aagcgcctcc gggtaacggg ttgtgggcat tatcggccgg gcgcgccatc
     1201 cctgcacacc cgggctgcat atcgcccgcc ctgtatccgc cgtgtaaggc ccggcggcag
     1261 gctcggaccg ttcagctcgt gccgcgttcg tccataattg gcgtgctcgc cgtcgtcgcc
     1321 gcgacaggcg cttgcgcaag cccttcccca ccctccgaca cgccgatgcc ggccacccgc
     1381 ccctcactcg tcgctcacgg cgggcccatc gtgatcgccc accgtggcgc cagcggctat
     1441 ctgcccgagc acacgctggc ggcctattac ctggcgatcc agcagggcgc ggatttcgtc
     1501 gaacccgatc tggtgatgac ccgcgacggg gtcctggtgg cgcgccacga gaacgagatc
     1561 ggtgcgacca cggacgtttc ggcgcatccc gagttcgtcg cgcgcaagac gcgcaagacc
     1621 atcgacggcg agcaggtgga gggctggttc accgaggatt tcacgctcgc cgagctgaag
     1681 accctgcgcg cgcgcgagcg gctgccgcgg ctgcgcccgg gcaacacgcg cttcgacggc
     1741 atgctcgaag tcgcgaccct ggacgaggtg ctggcgctgg tgcaggcgat ggactggcag
     1801 cgccgcatgc ttgcgcagca ggacggccgg accgcgccgc cgccgatcgg cgtgtatccc
     1861 gagaccaagc acccgagcta cttcgccggg ctgggcctgc cgatggaggc ggcgctggtg
     1921 cgcacgctgc agcgttgggg ctacgccggg cgcgacgccg cggcgttcat ccagtccttc
     1981 gagaccgcca atctcaaagc gctgcgccgc atgagcgagc tgcggctggt gcagctcgtc
     2041 gattccgggg gcaggccgca cgacttcgtg gtcgccggcg acccgcggcc agggtccgac
     2101 ctgctgacgc cgcgcgggct ggccgagatc gccggctacg cggatgccgt gggcgtgaac
     2161 aagtccctga tcattccgcg caacgcggac ggctcgctgg ccgcgcccac cacgctggtc
     2221 gcggacgccc acgccgcggg cctgctggta cacgcctgga cgttccgcgc cgagaacgag
     2281 ttcctgccgc gggagtttct gtcggggaac gatgcagccg tgctcggtga catggtcggc
     2341 gaagtgcggc gcttcctcga cgccggcgtc gacggcgtct tcaccgacca tcccgacttg
     2401 gggatcaaag cggtggatcg aagataaatt ccccctcacc tcagccctcc cgcaagggga
     2461 gagggaaacc tgggccgcgt ctagccgggc tgcaggtcga ctgggtggag atggcgctcg
     2521 aactgctcgg tggcggcgcg ccagctgaag cgctccgcga ccgcgcgcgc cgccgagcgc
     2581 tcgatgttca gggccgccag gcaggcgacg cgcagatcct cgtccagcac gccgcctccc
     2641 gagtccccga ccacgtccag cggaccggtg acgggatacg ccgccaccgg caggccgcag
     2701 gccatcgcct cgagcagcac taggccgaag gtgtcggtcc tgctggggaa gacgaacacg
     2761 tcggccgaag cgtacagctc ggccacctcg ctctggtcca ggaccccgag gtagtgggcc
     2821 tgcggatact tgatgcggat cgccggcagc gccgggccgt cgccgaccac ccacttcgag
     2881 cccggcagat ccagctccag gaaggcctcg acgttcttct ccaccgccac gcgcccgacg
     2941 tagagaaaga tcggcggcgt gctcttcaag cgctggcagc gccggaagct gaacacctcg
     3001 agatcgacgc cgcgggtcca ctggacgacg ttcttgaacc cccactgttg gaggtcccgc
     3061 accaccatcc gggtgggcgc catcactgcg ctcgacgccg cgtgaaagcg ccgcaggaag
     3121 gcgtaggtcc aggacagagg aaccgcgaag cgcgcgtgca cgtattcggg aaagcgcgtg
     3181 tggtaggcgg tcgtgaacgg catgccgatg cgccgcgcgc aagcgcgcgc cgccatgccg
     3241 agaggcccct cggtcgcgat gtgcagggcg ttcggccgga acgcccggat ccggcgcgcg
     3301 accttggcgc gggggaacag cgaaagtcgg atgtcgggat aggtcgggca ggggatggta
     3361 cgaaactcga gcggggtgat gaggccgacc tcgtggccca tcgcctgcaa ctcgcggcag
     3421 gtggatttca aggtgcgcac cacgccgttg acctgaggtt cccatgcgtc cgtcacgatg
     3481 agaatcctca tgctccgcgc tccggcgatc ggcgcaaccc gcgtgtcggc cgccggcgct
     3541 ttcacgaaag aaagggcgcc ttcgcagacg gagggagccc gacccttttg aacgcgatca
     3601 tgcgcgcgga ccatagccga ccagtattgc agtgtcatga acctaatgct gccgaaaggt
     3661 caaaacacgg cttggactgt aacgacggtg caatgcccat cggctagtct tgcgcgcagg
     3721 gagggggaag gctcgcgcta cttcaagagg taccggcctg aaccaggttg caagaaagct
     3781 gatcgacagc ctcgatcaga aggttttcga caccctctca ggtcgttccc tggtctacaa
     3841 cacctgctgg gaagatccgg ccgtcgatcg tcaggccctg tgcctgagcg catcggaccg
     3901 cttgctggtg atcacgagcg ccggctgcaa cacgctcgac tatgccctgc agggaccggc
     3961 gcgcatcgac gccgtcgacg ccaatccccg ccaaaccgcg ctgctcgagc tgaagctggc
     4021 cggcatccgg cggctcacgt tcgacgattt ctttcgcgtt ttcggggacg gctatcatcc
     4081 ggcgtttcgc gacctgtatc gcagcgcctt gcgcggcgag ctgtcgaatt tcgcgcgctc
     4141 gtactgggac cgcaacggcg actggttttg ccagaagcac gggcgcgaca ccttctatta
     4201 cttcggcctg tccggcctga tggcgcgcgc gtttcgcgcc tacctgcggg tgcgcccgag
     4261 actgcgcgcc gccatcgagg cggtgttcga cgccgggtcg ctgcccgccc agcgcgagat
     4321 ctacgacagc cagatcggcc cgctgctgtg gagccgtgcg atgaactgga cgctgtcgcg
     4381 ccagctcacc atgagcctgc tgggcgtgcc gcacacgcag cgcaaggaag tcgagcagca
     4441 gcaccccggc ggcgtggccg gcttcatccg ccaggcgatc gagtacgtgg cgcgcgagct
     4501 gccgctctcc agcaactact tctggagctt gtacctgcgt ggcgggtaca cgcgcgagtg
     4561 ctgccccgag tacctgaagc gcgacaactt cgcggcgctg aaaggcgggc tggtggactg
     4621 cattcatccg cataccgcga cggtgaccga gttcctcgat cgcgggcaca tgccgctgtc
     4681 gcgcttcgtg ctgctcgatc acatggactg gatgagcggt caccggccgc aggcgctggt
     4741 ggaggagtgg gaggcgatcc tgcgccgagc gtgctccggc gcccgggcga tcttccgcag
     4801 cgcgcacgcg cgaccgtcct acctggaagc ggtacatgtg gagcgtgacg gtcgcacgcg
     4861 gccgctgcgc gacctgctgc gctttcatcg cgcgctggcg cgccggctgt ccctgcagga
     4921 tcgcgtgcat acctacgccg ggtatcacat cgccgacatc gagaccgact gatgttgagc
     4981 accgccgacg cacacgtgct gtgggcgctg attcgagggc gctccagggc cgctacgcat
     5041 gcgcagcggc tccaggattt ctacgcggac caggcggacc ggtacgacgc gtttcgcgag
     5101 cggctcttgc agggaaggcg cgagctgatc gagcggctcc cggtggggcc cggcgcgcgc
     5161 gtcaccgagc tgggcgccgg gacggggcgc aacgtcgagt tcttcggcga gcggctggcg
     5221 cggctgcgat ccatcgtcct ggtcgatctg tgtcccgcgc tgatcgagca ggcgctgcgc
     5281 cgcaggcacc ggcttgccgc gtcgggcgtg tcgaacatca gcgtggttca cgccgacgcg
     5341 accacctatc ggccgtgcgg gccgcgcgat tgcgtgtact tctcctatgc gctgaccatg
     5401 atcccggact gggccggcgc catcgacaac gccatcgcga tgctgcgccc tgggggcgtc
     5461 ctgggggtgg tggatttcca tctgccggac cgcgtgcccg tgctggacgc cttctggcgg
     5521 cgctggttcg cgcacgacgg cgtctggttg tccgatcggc acctgccgta cctgcgccgg
     5581 cggctgcggc cgctgttcgc cagggaaggc cgggcccggc tgccgctgct gcccggcctg
     5641 cgggccccgt attacctgta catcggacag aaggcagcgg ccccggagat gggaggcgag
     5701 ggggagacgg gagactagac cgatggccga ggtgcgttcg atcttcctgt ccgacatcca
     5761 tctcggcacg cgcgcgtgcc aggccgaccg gctgctggat ttcctccggc acttcgagtc
     5821 gcgctacctg ttcctggtcg gggacatcct cgacttctgg gcgatgggtc agcgcggcgt
     5881 gtactggtcg gcgtcgatga acacgttcgt gcagaagatg ctcaagcgcg cccgccacgg
     5941 taccgaagtg atcttcatcc ccgggaatca cgacgaagcg ctgcgcgatt acctggacag
     6001 caccttcggc aacatcggcc tgcacctgga atacgagcac cggtcggcgg acggcaagcg
     6061 ctacctggtc ctgcacggag atcagttcga cgtcgtgacc aagtatcacc ggtggctcgc
     6121 cgtgctcggc gaccacggct acgctctgct ggtgcgcctc aacgtcctgt tgtcctgggt
     6181 gcgccgccag ctgggcatcg gcggctactg gtcgctggcg ggttacgcca agcgcaaggt
     6241 gaagacggcg gtgagcttca tctgcgactt cgaggaggcg gtggcccgcc acgtcaagga
     6301 gcgcggcctg gatggcgcga tctgcgggca catccacgcc gcggcgatca aacaagtcga
     6361 cggcgtgacc tacatcaact gcggcgactg ggtggacagt tgcacggcga tcgtggagta
     6421 cctcgacggg cgcatggagc tgatcgattg ggcggccgcg cctgcagccg ccggctcgag
     6481 tgaaacctcg cagcagcgct cgccggcgct ggccgctcac ggacgagcgg gctccgcggc
     6541 cgagcacgag acggtctgag gcacgccgcc tctactgcgg cagcaccttc accgccttga
     6601 cgaactcgag cgtgtaggcc tgccggtagt cgagatccgg cggcgccagc ccggcgcccg
     6661 ccatgaaatc gaaggtccgc ttccagcgcg cgtcggtcat ggtgagaatg ccctgcgtcc
     6721 tggcgtcgcc gccggtcacc aggccgaact cgcgcatctt gcgcagcccg aaggccatca
     6781 gctcgtcttc catctgcggg ttggccttct tgatcagcgc attgcccggc gccggctggt
     6841 tcaggtagct tttccagccc tccgcggtcg cctgcaggaa ccggcgcacc agatcgcgct
     6901 tgtcctgcgc ggtcttgcgc gtgacgacga tggtctgcgc atagggcgga tagccctggt
     6961 cggcgagtag gaacacaacc ggcttcaccc cggccttctc gatggagtag ggctcagagg
     7021 tggcatagcc ctgctgcgct acgttccggt ccactaggaa ctgctgcacg ctgaacgcgt
     7081 acggccgctt ctgcgcctcg gtgaagccgt acttggccct gagccagggc cagaaagtgg
     7141 tctcgctcgc ctgtccgatc agcagcgtgc gcgtcgcgag atcggccatc gtcttgacgt
     7201 ccgggtgcgc gatcagcacc gccgggtcct tctggaacgt ggcgcccacc gtcaccaccg
     7261 gcaagccctg ctcgatcagc ttgatggtct gcaggtcgta gcccatcacc aggtcggcct
     7321 ggccggcgag cagcagctgc accacgttga cctgcggtcc gcccattttc acgtcgacgt
     7381 ccaggccgtg cttgcggtaa gtgccctcgg ccaccgcctg atagaagccg ccgtgctcgg
     7441 cctgcgcata ccagttggtg aggaagatca gcttgggcgg cgattgcgca agcacaacgg
     7501 gtgcgtgcgt cgcccacagc agaacggcca tgccgagcag gcgccaggcg ccagggagcg
     7561 cggttgttct catggaggtc tccttgcggc ggaagcggca gcgtagcccc ggcgcgcgcg
     7621 gcgcgcaacc cggcgccgga ccggtagccc ggatgaagcc gcccaagacc tcgtcggcga
     7681 aacgggcgcc gcaatctggg gcaagggccc tcttccttcc cggattccgc tgcgctgcat
     7741 ccgggctaca tgaccgcgac cgcttcccga ctagcgcgcc agctcgctct cgtgccagc
//
LOCUS       DASPGE010000012         3712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1174889, whole genome shotgun sequence.
ACCESSION   DASPGE010000012 DASPGE010000000
VERSION     DASPGE010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174889"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VD791_00360"
     CDS             <1..583
                     /locus_tag="VD791_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HXV06456.1"
                     /translation="GRRVITLLAFGDALKPSAPAAIERLRELGVRTVLVSGDNRRAAQ
                     AVARRLKVDAVEAEVLPADKARIVEGLRGQGGKVAMVGDGINDAPALAAADVGIAMST
                     GTDVAMHTAGITLMRGDPVLVCDAIDVSRHTYAKIRQNLFWAFVYNVVGIPLAAFGLL
                     SPVIAGAAMALSSVSVVSNALLLKRWRPAAARR"
     gene            671..1060
                     /locus_tag="VD791_00365"
     CDS             671..1060
                     /locus_tag="VD791_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HXV06457.1"
                     /translation="MVAYGFGKQVKMSFDQAVQRVTQALAGEGFGVLTEIDVAATLKK
                     KLDKTIPPYKILGACNPNYASKALEAEPQIGLLLPCNVVVRQDAAGTVHVEFMDPHAV
                     LELVRRPEVDALGSEVRAKLQRVMAAL"
     gene            complement(1104..1772)
                     /locus_tag="VD791_00370"
     CDS             complement(1104..1772)
                     /locus_tag="VD791_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HXV06458.1"
                     /translation="MSFDGSRTMATCCEHDCEAAPAALEPRWRRVLWAALAINAVMFA
                     VEIGGSLIADSSALLADAVDFFGDAFNYGLSLAVLALSPLARARAAAVKGATMTGYGL
                     FVGGHAAWAWSQGVVPTPAAMGAVAILALLANASVALLLYRYRNGDANMRSVWICSRN
                     DVLGNLAVIAAAVGVFGTAQGWPDAVVAAILSVLAVSGGIAVMQHVRKELRPTMVRTG
                     PISG"
     gene            complement(1769..2434)
                     /locus_tag="VD791_00375"
     CDS             complement(1769..2434)
                     /locus_tag="VD791_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011326331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXV06459.1"
                     /translation="MSTRSPKREIFDNLARIGTALSSATRLEFLELLAQGERSVDQLA
                     SMTGVSVANTSQHLQKLRQAGLIVGRKEGQYVFYRLAGDEVVSLMAALGRVGETYLAE
                     VERVVRLYLAQRDDLEPIPARELLDRMRKGLVTVVDVRPPEEFAAGHLPGAVNIPVHA
                     LEKRLGELPKRKEVVAYCRGPYCLMSYDAVQLLRQKGLKARRLEAGLPEWRVAGLPVE
                     QMG"
     gene            2518..3363
                     /locus_tag="VD791_00380"
     CDS             2518..3363
                     /locus_tag="VD791_00380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HXV06460.1"
                     /translation="MITDIGMVSEPLIRLTAFVGVFLAVALWEGLAPRRRRAFPRLWR
                     WPHNLGLVAIDTLLARFLVPAGAVGVAVAAQSHGWGFAHQLAMPAWVAIPLAVVLLDL
                     TIYFQHVAFHAVPALWRLHRVHHADLDFDVTTGTRFHPIEILISAAIKFAAVAAIGAP
                     PIAVLAFEVLLNATAMFNHANASLPARLDAVLRWLVVTPDMHRVHHSVLPQETNSNFG
                     FNLPWWDRLFGTYRAQPKAGHEGMTIGIDAFRSPEDLRLDRLLLQPLRDTGGGYAITP
                     RRAAS"
     gene            3409..>3712
                     /locus_tag="VD791_00385"
     CDS             3409..>3712
                     /locus_tag="VD791_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HXV06461.1"
                     /translation="MKTLFILNDPPYGTERSYNGLRLAGALAKREGEEVRVFLIGDAA
                     ASAKRNQKVPQGYYNLEVMLGAVTRRGGEVGVCGSCMDARGITEEDLVPGTRRSSME"
BASE COUNT          605 a         1257 c         1266 g          584 t
ORIGIN      
        1 cggtcggcgt gtgatcaccc tgctggcgtt cggcgatgcg ctcaagccct cggcaccggc
       61 cgcgatcgag cggctgcgcg agctgggcgt gcgcacagtt ctggtgagcg gcgacaatcg
      121 gcgcgcggcc caagcggtgg cgaggcggct gaaggtggac gccgtggagg cggaggtgct
      181 cccggccgac aaggcgcgca tcgtcgaggg gctgcgcggg cagggcggga aagtggcgat
      241 ggtgggcgac ggcatcaacg acgcgccggc gctcgccgcg gcggacgtgg gcatcgccat
      301 gtcgaccggc accgatgtgg ccatgcacac ggccggcatc accctgatgc gcggcgatcc
      361 cgtgctggtg tgcgatgcca tcgacgtgtc gcgtcacacc tacgcgaaga tccgccaaaa
      421 cctgttctgg gccttcgtct acaacgtcgt cggcattccg ctggcggcgt tcggtctgct
      481 cagcccggtg atcgccgggg cggcgatggc gctgtccagc gtgagcgtgg taagcaacgc
      541 cctgctgctc aagcgctggc ggccggcggc ggcgcgacgc taaggcacgc gcccgcgaga
      601 aggaatgcga ccgcggtcac agaccgcaga gcacccgtcg ggcgatagtg cgattttcga
      661 ggagattccc atggtggcgt acggtttcgg caaacaggtg aagatgagct tcgatcaggc
      721 ggttcagcgc gtaacgcaag cgctggccgg cgagggtttc ggcgtgctga ccgagatcga
      781 cgtcgcggcc accctgaaga agaagctcga caagaccatc ccgccctaca agatcctggg
      841 cgcctgcaac ccgaactacg cttccaaggc gctcgaggcc gaaccgcaga tcggtttgct
      901 gctgccgtgc aacgtcgtgg tccgccagga cgccgcgggc acggtgcacg tggagttcat
      961 ggacccgcac gcggtccttg agctggtgcg gcgtcccgag gtggacgcgc tgggcagcga
     1021 ggtgcgcgcc aagctccagc gggtgatggc ggcgctgtga attggcgctg ggggctctgg
     1081 agggtggcgc gatgcgctca cgctcacccg gagatgggcc ccgtccgcac catcgtcgga
     1141 cggagctctt tccggacatg ttgcatcacc gcgattccgc cggacaccgc tagcacggag
     1201 aggatcgcag ccaccaccgc atccggccag ccttgcgcag tgccgaacac gccgacggcg
     1261 gctgcgatca cggccaggtt cccgagcacg tcgttgcgcg agcagatcca cactgaacgc
     1321 atgttggcgt cgccgttgcg atagcggtag agcagcaggg ctacgctcgc gttcgccaac
     1381 aatgcgagga tcgcgaccgc gcccatggcg gcgggggtcg gcacgacacc ctggctccag
     1441 gcccaggcgg catgcccgcc gacgaacagg ccgtagccgg tcatcgtcgc acccttcacc
     1501 gcggccgcgc gagcgcgtgc aagcggactg agcgcgagca ccgccaggga cagcccgtag
     1561 ttgaaggcgt cgccgaaaaa atccacagcg tcggcgagca gcgcgctgga gtctgctatc
     1621 aggctgccgc cgatctcgac cgcgaacatc accgcattga tcgcgagcgc agcccacagc
     1681 acgcgacgcc agcgcggctc gagcgccgcg ggagcggctt cgcaatcgtg ttcgcaacag
     1741 gtcgccatcg tccgagatcc gtcgaagctc aacccatctg ctccaccggc agaccggcaa
     1801 cgcgccattc gggcagcccg gcttcgagcc ggcgcgcctt cagtcctttc tggcgcagca
     1861 actgcaccgc gtcgtaggac atcaggcagt aaggcccgcg gcagtaggcc accacctctt
     1921 tgcgcttggg cagctctccc agccgcttct cgagcgcgtg cacggggatg ttcaccgcgc
     1981 cgggcaggtg cccggcggcg aactcttccg gtgggcgcac gtcgaccacg gtgaccagcc
     2041 ctttgcgcat tcgatccagc agctcccggg ccgggatcgg ctcgaggtcg tcgcgctgcg
     2101 ccaggtacag tcgcacgacg cgctcgacct cggccagata ggtctcgccc acgcgcccga
     2161 gcgcggccat cagcgacacc acttcatcgc ccgccaggcg gtagaagacg tactgtccct
     2221 ccttgcgccc cacgatcagg cctgcctggc gcagcttctg cagatgctgc gaggtattcg
     2281 ccaccgacac gccggtcatg gacgcgagct gatccacgct gcgctcccct tgggccagca
     2341 gctcgaggaa ctccaggcgc gtggcgctcg acagggcggt cccgatcctg gcgaggttgt
     2401 cgaagatttc gcgtttcgga ctgcgggttg acatctggcc gggttcgcaa tagtattcaa
     2461 tcgatcaatt gaatgaatgt aacatcgagc gcggcccgtg gcaagcgggg caagatcatg
     2521 atcactgaca tcggcatggt atccgagccg ctgatccggc tcaccgcctt cgtcggcgta
     2581 ttcctggcgg tggccctctg ggaggggctc gcaccgcgcc gtcggcgcgc ctttccgcgg
     2641 ctgtggcgct ggccgcacaa cctcgggctg gtggccatcg acacgctgct cgcacgattc
     2701 ctggtgccgg ccggcgcggt cggcgtggcc gtggccgcgc agtcccacgg ctgggggttc
     2761 gcccaccagc tcgcgatgcc ggcgtgggtc gcgatcccgc tggctgtcgt cctgctcgat
     2821 ctgaccatct acttccagca cgtggcgttt cacgcggtgc cggccctgtg gcgcctgcac
     2881 cgcgtgcatc acgccgacct cgacttcgac gtcaccaccg gcacgcgctt ccaccctatc
     2941 gagatcctga tctccgccgc catcaagttc gccgccgtcg cggccatcgg cgcgcccccg
     3001 atcgcggtgt tggccttcga ggtgctgctc aacgccaccg cgatgttcaa ccacgcgaac
     3061 gccagtctgc ctgcacgcct ggacgccgtg ttgcgctggc tggtggttac gcccgacatg
     3121 catcgcgtac accattcggt cctgccgcag gagaccaaca gcaatttcgg cttcaaccta
     3181 ccgtggtggg atcggctgtt cggcacctac cgcgcccagc cgaaggccgg gcatgaaggg
     3241 atgacgatcg ggatcgacgc atttcgctcc ccggaggatc tccggctgga tcgcctgctg
     3301 ctgcagccgt tgcgggatac agggggaggg tatgccatca ccccgcgccg ggcggcgagt
     3361 tgagcgtccc agaccgaacc gccctgcgta gaccgaaagc aagtccggat gaagacgctg
     3421 ttcattctga acgacccgcc ctacgggacg gaacgcagct acaacggact gcggctcgct
     3481 ggcgcgctgg caaagcgcga gggcgaggag gtgcgcgtgt tcctgatcgg cgatgcggcg
     3541 gccagcgcca agcgcaacca gaaggtgccg cagggctact acaacctgga ggtgatgctc
     3601 ggtgcggtca cacgccgcgg cggggaggtc ggcgtgtgcg gttcgtgcat ggacgcgcgc
     3661 ggcatcaccg aggaagacct ggtgccaggc acccgacgca gcagcatgga ag
//
LOCUS       DASPGE010000013         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_4481, whole genome shotgun sequence.
ACCESSION   DASPGE010000013 DASPGE010000000
VERSION     DASPGE010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4481"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..760
                     /locus_tag="VD791_00390"
     CDS             107..760
                     /locus_tag="VD791_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HXV06462.1"
                     /translation="MTGERILVQVFAKAPVPGEVKTRLIPVLGREGATELYCRLVQHT
                     LATVAMARVGSIELWTTAPRSEVFLLACERLLGIPLLPQPDGDLGARMSAAATHGLAR
                     ARAVLLVGSDVPTLCCDDLRAARDALANGSDAVLGPAEDGGYWLLGLSRHATELFQDL
                     PWSTPAVLDATRERLRVLRWRWHELPTRWDVDRPEDLQRLAGEPSLGRLVRDLPKTA"
     gene            757..1926
                     /locus_tag="VD791_00395"
     CDS             757..1926
                     /locus_tag="VD791_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXV06463.1"
                     /translation="MKRLILVGGGHTHVEVLRRFGMRPLAGIELLLISPGRFTPYSGM
                     LPGLVAGHYGFEECHIDLEALTNFARARFLRDQTVGLDPEAKSLRCESGVSVPYDLLS
                     IDIGAVPARYPKGRHPQGDSTGVGIPVKPVDRYLQEWERVLQLARQRPLDLLVVGGGA
                     GGVEITLAMQHRLSRLTPRPSVRFSLVTMTDTIMPDHCAGVRKRLARVLAQRGVAVHL
                     RSRVARQEGDGVLLESGARLQADCVFSALGARPPAWLAQAGLKTDAAGFVLVGETLQS
                     LSHPEVFAVGDVATMARHPRPKSGVYAVRQGPPLADNLRRLAGGQVLEPYLPQPIALN
                     LISTGDQYAVASWGSLAWEGAWVWRWKDRIDRRFMRRYRFGAAGTRPGRGAGFMR"
BASE COUNT          246 a          685 c          759 g          317 t
ORIGIN      
        1 ggcgtgtggc gcaccatggc gctgatgtgg tggctgcggc tgcggtattt cctcggcgcc
       61 tcgccggcgc gcctgcgccg gctctacgaa ggcgagcgca cctgagatga ccggcgagcg
      121 catcctggtg caggtgttcg ccaaggctcc cgtgccgggc gaagtgaaga cgcgcctgat
      181 cccggtgctg ggccgcgagg gtgctaccga actgtattgc cgcctggtgc agcacacgct
      241 cgcgacggtg gcgatggcgc gcgtgggcag catcgagctg tggaccacgg cgcctcggag
      301 cgaggtgttc ttgctcgcct gcgagcgcct gctcggcatc ccgctgctgc cgcagcccga
      361 cggcgacctg ggtgcacgca tgagcgccgc ggcgacgcat ggcctggcgc gtgcgcgtgc
      421 cgtcctgctg gtcggcagcg acgtgccgac cctgtgctgc gacgacctgc gcgcagcgcg
      481 cgacgcgctg gcgaacggca gcgacgccgt gctggggccg gccgaggacg gcggctactg
      541 gctgctcggt ctgtcccgcc acgcgaccga gctgttccag gacctgccct ggagcacccc
      601 ggcggtgctg gatgccacgc gcgagcgcct gcgcgtgctg cgctggcgct ggcacgagct
      661 gccgacgcgc tgggacgtgg accggcccga ggacctgcag cgactggccg gcgaaccctc
      721 cctcggcagg ctcgtcagag atctgcccaa gaccgcgtga agcggctgat cctcgtcggc
      781 ggcggacaca cccatgtgga ggtgctgcgc cggttcggga tgcggccgct cgccggcatc
      841 gagctgctgc tgattagccc cggccgcttc acgccgtact cgggcatgct gcccggcctg
      901 gtcgcgggcc actacggctt cgaggagtgc catatcgacc tggaagcgct gaccaacttc
      961 gcccgcgcgc gcttcctgcg cgaccagacg gtggggctcg atccggaggc gaagtcgctg
     1021 cgctgcgaat ccggcgtgtc ggtgccctac gacctgctct cgatcgacat cggggcggtg
     1081 cccgcgaggt atccgaaggg gcgacatccg cagggcgact ccaccggcgt gggaatcccg
     1141 gtgaagccgg tggaccgcta cctgcaggag tgggagcgcg tgctgcagct tgcgcgacag
     1201 cgcccgctcg acttgctcgt ggtcggcggg ggtgcgggcg gggtggagat cacgcttgcc
     1261 atgcagcatc ggttgagccg gctcacgccg cggccgtcgg tgcgcttctc gctggtgacc
     1321 atgaccgaca ccatcatgcc cgatcactgc gccggcgtgc gcaagcgctt ggcgcgggtg
     1381 ctcgcgcaac gcggagtcgc cgtgcatctg cgcagccgcg tcgcgaggca ggaaggcgac
     1441 ggcgtgctgt tggaatcggg cgcgcgcctg caggccgatt gcgtgttctc ggcgctgggg
     1501 gcgcggcccc cggcgtggct cgcgcaggcg ggcttgaaga ccgacgccgc gggcttcgtg
     1561 ctggtgggcg agacgctgca gtcgctgtcg cacccggagg tgttcgccgt cggcgacgtc
     1621 gccaccatgg ccaggcaccc gcgccccaag tcgggcgtgt acgccgtgcg ccagggcccg
     1681 ccgctggcgg acaatctgcg ccgcctggcg ggcggccagg tgctcgagcc ctatctcccg
     1741 cagccgatcg cgctcaatct gatcagcacc ggcgaccagt acgcggtggc gtcctggggt
     1801 tcgttggcct gggagggcgc ctgggtttgg cgctggaagg accgcatcga ccggcgcttc
     1861 atgcggcgct accggttcgg cgcggcggga acgcggcctg gaaggggtgc cggcttcatg
     1921 cgatagtggc gacccttcgc gttgagggcc cccgcatgct cacggcctcg gatcggtcgc
     1981 tgctcttcgg atgcctcggt tatgccg
//
LOCUS       DASPGE010000014         7533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1644222, whole genome shotgun sequence.
ACCESSION   DASPGE010000014 DASPGE010000000
VERSION     DASPGE010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7533
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644222"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VD791_00400"
     CDS             <1..1572
                     /locus_tag="VD791_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXV06464.1"
                     /translation="FADNSLEGTQTSPLSLLAVDREVPYTYPDITDNRLDFVNLRLSK
                     VFDNDKILAGNVYYRRFKGENFSSNVNDECADVAINPGNCAGGSPAEPQGSNDTSEID
                     TDGFGATLQFTLLKQLFGRENKFTVGASYDAGRTRFTQFEQIADFSADRGTVATGPFE
                     LETDIDADNDYYGIYATDTFSINAQTHLTLSGRFNRAEVTLRNRSGDPADDDLNGDHH
                     FTRFNPAIGLNWNPNARFNTWAGYNEGMRVPTPMELTCADEADPCKLPNAFLADPPLD
                     PVISRTIELGTRGAITANTFYSLSAYRTDLQDDIQFITVSGVNGFFDNVGDTRRQGVE
                     IGLQTRLGKLALSAAYGFVDATFRDSFLVASPNNSQADANDRIQVASGDRIPGIPQHN
                     FKLRADYAFTPRLFGGLNFVYASDQYARGDENNEDVNGTVPDYFVVNLDARWQVTKEL
                     QVFGRITNLLDEEYETFGVLGENFFPAPAFAFDAANAVDEQFRTPAAPRGFFVGVRYD
                     FGRPARTAGTGGIDD"
     gene            1784..2488
                     /locus_tag="VD791_00405"
     CDS             1784..2488
                     /locus_tag="VD791_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HXV06465.1"
                     /translation="MEIKALTFDVFGTVVDWRGSIVREGHALGRARGLEVDWPAFADA
                     WRAGYRPAMDRVRRGELPWTNIDTLHRRILDELLPRFGIRSLKAAEKDHLNRVWHRLR
                     PWPDAVRGLRRLKTRYVIATLSNGNVALLTNMAKHAGLPWDCVLSAELFGRYKPDPET
                     YRGAAVLLGLKPREVMMVAAHPDDLAAARRCGLRTAFVPRPREHGTARASQPPANAGF
                     DIIASDFIDLARQLRT"
     gene            2553..3998
                     /locus_tag="VD791_00410"
     CDS             2553..3998
                     /locus_tag="VD791_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06466.1"
                     /translation="MLLPGCATFRSYDAELSATLAMAADGRVDAALKTLDANNKRDRK
                     DLLYFLERGELLRLKTDYRDSQSAWGAADARVQEWERTAKTDPDKLLGSAGSYLVNDR
                     LRPYEGHDYEKVMLTTRMAMNFLGQGDFANARVAIKQTHEREALIAEVRARQYAKVQE
                     EAGKRDAAPPTFKEINGYPVQEIDNPEVSALRNSYQSALSHYLAGFVYEALREPSLAA
                     AGYRQAIELQPGTGLLEESLAGLDQRVAAPDDGLTDTLVVIESGLVPARRSQQFALPI
                     PVGGELVLVAVAFPVMQARSEAFVPDEVRVDFGEPLRPARITSVDAMARRALQDEMPG
                     IVLRSVVRSTTKAVAQYQAQRAAEQQRMHGDSGEAGLLGLAALALMIGTVATEQADER
                     GWRSLPADIYVARGKLPRGRRMLSLATPRGERSVQVDLSGRHAFIALRYLQGSLFTML
                     PQIPASGGGDGAQTVALPADATQLNPKEVSP"
     gene            3995..4435
                     /locus_tag="VD791_00415"
     CDS             3995..4435
                     /locus_tag="VD791_00415"
                     /inference="COORDINATES: protein motif:HMM:NF018889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1425 domain-containing protein"
                     /protein_id="HXV06467.1"
                     /translation="MKIRALVVLAAACALLALAGCGAKKGFEKKGFAYGCPIGETKVG
                     SMEDLVQSKVAREGPRTNVAVTEMRCTMSGDLLRIDANLNNDASDTRRVAYKFRWIDR
                     EGMRAAEEEVWKPILMYEKSNQLVSAVAPTPKAIDFRLIYMAQD"
     gene            4468..5058
                     /gene="lpoB"
                     /locus_tag="VD791_00420"
     CDS             4468..5058
                     /gene="lpoB"
                     /locus_tag="VD791_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HXV06468.1"
                     /translation="MCHSVRIGRLAAVTLAAGLALAGCAKEQVRYGDEKGVETVTNQF
                     GSTDLQMIAESMTRSLLQAPVVVSSNLPVVTVQEVKNKTSEYIDTRAITDSIRAELQK
                     SGKVRFAVDAAAMEQQVEELERQQSELYDKDKAVEVGRMTGAQYRLEGNITSIVKQAK
                     DVKDVYYKFNLQMWNIRNGLLEWSDEKEIRKTTSKG"
     gene            complement(5066..6184)
                     /locus_tag="VD791_00425"
     CDS             complement(5066..6184)
                     /locus_tag="VD791_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06469.1"
                     /translation="MAPDSPPLSSSPAQAAHGRRLRFTAVQTLVAMGLLSAFPGVVLA
                     LLLLGAQQRDIEAVDKRVRGQEYGAAVSRLLSAVHWHRDLSSRALLGDTSVEGTLRRA
                     QTELRSALRELDAAAAQEGFAFALIDRWGELRAQWARVESEQPTLTPAANVEAHNRLL
                     AEAGVFLALVGQRSELLLDPAPGVHALSVCSTLTMPALARHTGMLRAYALLTAREVSP
                     AQRERIVRTLADARTAFDQVVFSLDEAYAADGKTRAALQERTTRALEQVRRVLDQAET
                     AFAPGRRPDLDANAWFSATTQAVEALEGARDAAAARVSSLLSKRARGLRERRLVTQAL
                     AVLVIAGTLLGVLVLGRSLLRGRAPPLTPPLLMAGRTP"
     gene            complement(6331..6807)
                     /locus_tag="VD791_00430"
     CDS             complement(6331..6807)
                     /locus_tag="VD791_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HXV06470.1"
                     /translation="MTMTITSTAFAADASIPKRYTCEGDDVSPPLAWSGVPAAAKSLA
                     LIVDDPDAPDPAAPRMTWVHWVLYNLPVASGGLPEAVSAARFPAGTVEGLNDWQRTGY
                     GGPCPPIGRHRYFHKLYALDVVLPDLKRPNKAALEKAMKGHIVAEAQLVGTYQKSR"
     gene            complement(6885..>7533)
                     /gene="queG"
                     /locus_tag="VD791_00435"
     CDS             complement(6885..>7533)
                     /gene="queG"
                     /locus_tag="VD791_00435"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HXV06471.1"
                     /translation="LAAQAGLGWRGKHTLLLDRSAGSWFFLGEIYTDLPLPLDQPVSE
                     HCGTCTRCIDVCPTRAIVAPYLLDARRCISYLTIEHKGDIPEELRPLLGNRIYGCDDC
                     QLVCPWNSFAGTAEIDDFAVRNGLDAAALVELFAWTQDDFDRRLEGSAIRRIGYERWL
                     RNLAVALGNAPSGPQVVAALRARADHPSALVRSHVLWALRRHEAPKPGAPRAEPA"
BASE COUNT         1254 a         2624 c         2600 g         1055 t
ORIGIN      
        1 ttcgccgaca acagcctcga gggcacgcag acctcgccgc tctcgctgct ggcggtggac
       61 cgcgaagtac cctacaccta cccggacatc accgacaacc ggctggactt cgtgaacctg
      121 cgcctgtcga aagtgttcga caacgacaag atcctcgccg gcaacgtcta ttaccgccgc
      181 ttcaagggcg agaacttcag cagcaacgtg aacgacgagt gcgcggacgt cgccatcaat
      241 cccggcaact gcgcgggagg ctcgccggcc gagccgcagg gctccaacga caccagcgag
      301 atcgacaccg acggcttcgg cgccaccctg cagttcacgc tgctcaaaca actgttcggc
      361 cgcgaaaaca agttcacggt gggagcgagc tacgacgccg ggcgcacgcg cttcacacag
      421 ttcgagcaga tcgccgactt cagcgccgac cgcggcacgg tggccaccgg tcccttcgag
      481 ctggagaccg acatcgacgc cgataacgac tactacggca tctacgccac cgacacgttc
      541 tcgatcaacg cgcaaacgca cctgacgttg tccggccggt tcaaccgtgc ggaggtcacg
      601 ctgcgcaacc gcagcggcga cccggccgac gacgacttga acggcgacca tcatttcacg
      661 cgcttcaacc cggcgatcgg cttgaactgg aaccccaatg ctcgcttcaa cacctgggcg
      721 ggctacaacg aaggcatgcg cgtgcccacg ccgatggagc tgacctgcgc ggacgaggcc
      781 gatccctgca agctgccgaa cgcgttcctg gccgacccgc cgctcgaccc ggtgatctcg
      841 aggaccatcg agctgggtac gcgcggcgcg atcaccgcca acaccttcta cagtctgagc
      901 gcgtaccgca ccgacctgca ggatgacatc cagttcatca cggtaagcgg ggtcaacggc
      961 ttcttcgaca acgtcggcga cacgcgccgc cagggcgtcg agatcggatt gcagacccgc
     1021 ctgggcaaac tggcgctcag cgcggcttat ggtttcgtcg acgccacctt ccgtgactcg
     1081 ttcctggtgg cgagtccgaa caactcgcag gccgacgcca acgaccgcat tcaagtcgcg
     1141 tcgggcgacc gcatcccggg catcccgcag cacaacttca agctgcgcgc cgactacgcc
     1201 ttcacgccgc gcctcttcgg tggcctcaac ttcgtctacg ccagcgatca atacgcgcgc
     1261 ggcgacgaga acaacgagga cgtgaacggg acggttccgg actacttcgt ggtcaacctg
     1321 gatgcgcgct ggcaggttac caaagagctg caggtgttcg gccggatcac caacctgctc
     1381 gacgaggaat acgaaacttt cggcgtgctg ggcgagaact tcttccccgc ccccgccttc
     1441 gccttcgacg ccgccaacgc ggtggacgag cagttccgca cgcccgccgc cccgcgcggc
     1501 ttcttcgtgg gcgtgcgcta cgacttcggc agaccggcgc gcacagccgg caccgggggt
     1561 atcgacgact gaccggcaag cgcggccgcg cccgctcgca agggcgtacg ctgcgcggat
     1621 accggccgca gtcgccggcc gcgccgccgc gctcgaccgg cggctcccgc atggattatc
     1681 ctgacgcgcg ctgcggcccg gtcaaccgcc gggcgcggcg cgtcgttgct cgagggccgc
     1741 gacgtccggt acccgacggc ccgacgatcg ctctgcaacc cctatggaaa tcaaagcgct
     1801 gaccttcgac gtcttcggca cggtcgtcga ctggcgcggc agcatcgtcc gcgaggggca
     1861 cgcgctgggc cgcgcgcgcg ggctggaggt ggactggccg gcattcgccg acgcctggcg
     1921 cgcgggctac cggccggcca tggaccgcgt gcgccggggt gagctgccgt ggaccaacat
     1981 cgataccctg caccgccgga tcctggacga gctgctgccc cggttcggca ttcgcagcct
     2041 gaaggcggcc gagaaagacc acctcaaccg ggtgtggcac cggttgcggc cgtggccgga
     2101 tgcggtgcgc gggctgcgcc gcttgaagac gcgctacgtc attgctaccc tgtccaacgg
     2161 caacgtcgcg ctgctcacca acatggcgaa gcacgccggc ctgccatggg attgcgtgct
     2221 ctcggccgag ctgttcgggc gctacaagcc ggacccggag acctaccgtg gagcggccgt
     2281 gctgctgggc ttgaagccgc gcgaggtgat gatggtggcg gcgcaccccg acgatctggc
     2341 cgccgcgcgc cgttgcggac tgcgcaccgc cttcgtgccc cgcccgcgcg agcacggcac
     2401 ggcgcgtgct tcgcaaccgc ccgcgaatgc aggcttcgat atcattgcat cggacttcat
     2461 cgacctcgcc cggcaattgc ggacttgagc ggcgccggcg cgcgctgccc ggacggtgcc
     2521 ggtttgccgg ggccggcctg atcctggcga tcgtgctgct gcccggctgc gccaccttcc
     2581 gcagttatga cgccgagttg agcgcgacgc tggcgatggc cgccgacggc agggtggacg
     2641 cggccctcaa aaccctcgac gccaacaaca agcgcgaccg caaagacctg ctctatttcc
     2701 tggagcgcgg cgagctgctg cggctgaaga ccgactaccg cgacagccag agcgcctggg
     2761 gcgccgccga cgccagggtc caggaatggg agcgcaccgc caagaccgat ccggacaaac
     2821 tgctgggcag cgccggcagc tacctggtca acgaccggct gcggccttac gagggtcacg
     2881 attacgagaa ggtgatgctg accacgcgca tggcgatgaa cttcctggga cagggcgact
     2941 tcgccaacgc gcgcgtggcg atcaagcaga cgcacgagcg cgaggccctg atcgccgagg
     3001 tgcgcgcccg tcagtacgcc aaggtgcagg aagaagcggg caagcgcgac gccgccccgc
     3061 ccaccttcaa ggagatcaac ggttacccgg tgcaggagat cgacaacccc gaggtgagcg
     3121 cgctgcggaa cagctatcag agcgcgctca gccactatct ggcgggcttc gtgtacgagg
     3181 cgttgcgcga gcccagcctg gccgctgcgg gctaccgcca ggcgatcgag ctgcagcccg
     3241 gcacggggtt gctggaggaa tcgctcgctg ggctggacca gcgtgtggcg gcccccgacg
     3301 atggcctgac tgacaccctg gtagtcatcg agtccgggct cgtcccggcg cggcgctcgc
     3361 agcagttcgc gctgccgatc ccggtgggcg gcgagctggt gctggtggcg gtggcgttcc
     3421 ccgtgatgca ggcgcgtagc gaggctttcg tgccggacga ggtgcgcgtc gatttcggcg
     3481 aaccgctccg gccggcgcgc atcaccagcg tggacgcgat ggcgcggcgc gctctgcagg
     3541 acgagatgcc cggcatcgtg ctgcgcagcg tggtacgctc caccacgaag gcggtggcgc
     3601 agtaccaggc gcagcgcgcc gcggagcagc aacggatgca cggcgatagc ggcgaggccg
     3661 ggctgctcgg gctggccgcg ttggccctga tgatcggcac ggtggcgacc gaacaggccg
     3721 acgagcgcgg ttggcgcagc ctgccggccg acatctacgt ggcccgcggc aagctcccgc
     3781 gcgggcgccg catgttgtcg cttgccaccc cgcgcggaga gcgtagcgtg caggtcgacc
     3841 tatccgggcg tcatgcgttc atcgccttgc gctacctgca aggcagcctg ttcaccatgc
     3901 tgccccagat tccggcgtcg ggcggcgggg acggcgcgca aaccgtcgcc ctcccggccg
     3961 atgcgactca actcaacccg aaggaggttt cgccatgaag atccgagcgc tcgtcgtgct
     4021 cgccgccgcc tgcgcgctgc tcgccctcgc cggctgcggc gccaaaaagg gcttcgagaa
     4081 gaagggcttc gcctacggtt gcccgatcgg ggaaacgaag gtcggctcga tggaggacct
     4141 ggtccagtcc aaggtcgcgc gcgaaggccc gcgcaccaac gtggcggtga ccgagatgcg
     4201 ctgcaccatg agcggcgatc tgctcaggat cgacgccaat ctcaacaacg acgcctccga
     4261 cacgcggcgc gtggcctaca agttccgctg gatcgatcgc gagggcatgc gcgccgcgga
     4321 agaggaggtc tggaagccga tcctgatgta cgagaagtcc aaccagctgg tcagcgccgt
     4381 ggcgccgacc cccaaggcga tagacttccg cttgatctac atggcgcagg actgatgtcc
     4441 tcgcctacgc gcaaaggaga accccgcatg tgccattccg ttcgcatcgg ccgcctggcg
     4501 gccgtgacgc tcgccgccgg cctggcgctc gccggctgcg ccaaggagca ggtccgctac
     4561 ggcgacgaaa aaggcgtgga gacggtgacc aatcagttcg gctccaccga cctgcagatg
     4621 atcgccgagt cgatgacgcg ctcgctgctg caggcgccgg tggtggtgag cagcaacctg
     4681 ccggtcgtga ccgtgcagga ggtcaagaac aagaccagcg agtacatcga cacgcgcgcg
     4741 atcaccgaca gcatccgcgc cgagctgcag aagagcggca aggtgcgctt cgcggtggac
     4801 gccgcggcca tggagcagca ggtcgaggag ctggagcgcc agcagagcga gctgtacgac
     4861 aaggacaaag cggtggaggt gggccgtatg acgggtgcgc agtaccggct ggagggcaac
     4921 atcacctcca tcgtcaagca ggcgaaggac gtgaaggacg tctactacaa gttcaacctg
     4981 cagatgtgga acatccgcaa cggcctgctc gagtggtcgg acgagaagga gatccgtaag
     5041 accaccagca agggctgaga cgcactcaag gcgtgcgccc cgccatcagc agcggcgggg
     5101 tgagcggcgg cgcccggccg cgcagcaggc tcctgccgag caccagcacg ccgagcaggg
     5161 tgccggcgat caccagcaca gccagcgcct gagtcaccag gcgccgctcg cgcaggccgc
     5221 gggcgcgctt ggacagcagc gacgagacgc gggcggcggc ggcatcgcgt gcgccctcca
     5281 gcgcctccac tgcctgggtc gtggccgaga accaggcgtt ggcatccaga tcgggccggc
     5341 gccccggcgc gaaagcggtt tcagcctgat ccagcacccg gcgcacctgc tcgagggcgc
     5401 gtgtggtgcg ctcctgcagt gcggcgcgcg tcttgccgtc ggccgcgtag gcctcgtcga
     5461 ggctgaacac cacctggtcg aacgcggtgc gtgcatcggc aagcgtgcgc acgatccgct
     5521 cgcgctgggc ggggctcacc tccctggccg tcaacagagc gtaggcgcgc agcatgccgg
     5581 tgtgccgggc gagcgccggc atggtcagcg tgctgcacac gctcagggcg tgcacaccgg
     5641 gcgcggggtc gagcagcagc tccgagcgct gcccgaccag cgccaggaac acgcccgcct
     5701 cggccagcag cctgttgtgc gcctccacgt tggccgcggg cgtgagcgtg ggctgctccg
     5761 attcgacccg cgcccactgc gcccgcagct cgccccagcg gtcgatcagc gcaaacgcga
     5821 aaccctcctg cgccgccgcc gcatcgagct cccgcagcgc cgagcgcagc tccgtctggg
     5881 cgcgccgcag cgtgccttcc accgaggtgt cgcccagcag cgcgcggctc gacaggtcgc
     5941 ggtgccagtg caccgcgctc agcaggcgcg acaccgcggc gccgtactcc tgcccgcgca
     6001 cgcgcttgtc caccgcctcg atgtcgcgct gttgcgcacc gagcagcagc agggcgagga
     6061 ccaccccggg aaaggcggac agcagaccca tcgccaccag cgtttgcacc gcggtgaacc
     6121 gcaggcgccg gccatgcgcg gcctgcgcgg gactcgagga caagggcggg gagtcgggtg
     6181 ccatgagaag accgcgcggg attgcggcgc attgtcgctt gtaacggaca ccgcgccgcc
     6241 gaacttaagg gtgcgtccgg gcccggagtt tgccctgcgc cccctgctgg gctgaggagc
     6301 ccggccggtt ccggccgatc ccggacggtc tcagcggctt ttctgatagg tgccgaccag
     6361 ttgcgcttcg gccacgatgt ggcccttcat cgccttttcc agggccgcct tgttcgggcg
     6421 cttgaggtcg ggaagaacga cgtccagggc gtacagcttg tggaagtagc ggtgccggcc
     6481 gatcgggggg caggggccgc cgtagccggt gcgctgccag tcgttcaggc cctccacggt
     6541 accggccggg aagcgcgcgg cgctcaccgc ctcgggcaga ccgccggagg cgaccgggag
     6601 gttgtagagc acccagtgca cccaggtcat gcggggggcg gccgggtcgg gcgcatccgg
     6661 atcgtcgacg atcagcgcca ggctcttcgc cgccgccggc acgccgctcc acgccagcgg
     6721 cggggacacg tcgtcgccct cgcaggtgta gcgcttgggg atggatgcgt cggccgcgaa
     6781 cgcggtcgag gtgatggtca tggtcatggc tttctcctcg gcaagggcga cgccgggcgt
     6841 cggcagcagc gatgcgagca gcgcaatcgc gaatgcgcgc gtcttcatgc cggttccgcg
     6901 cgcggggctc ccggcttcgg cgcctcgtgc cggcgaagcg cccagagcac gtggctgcgc
     6961 accagcgcgg acggatggtc ggcgcgcgcg cgcagcgccg cgaccacctg cgggccggac
     7021 ggcgcgttgc cgagcgccac cgcgaggttg cgcagccacc gctcgtagcc gatgcggcgg
     7081 atcgcgctgc cctccaggcg ccggtcgaag tcgtcctgcg tccacgcgaa cagctccacc
     7141 agcgccgccg catccagccc gttgcgcacg gcgaaatcgt cgatctcggc ggtgcccgcg
     7201 aagctgttcc aggggcacac cagctggcaa tcgtcgcagc cgtagatgcg gttgccgagc
     7261 aggggccgca actcctcggg aatgtcgccc ttgtgctcga tggtgaggta ggagatgcag
     7321 cggcgcgcat ccagcaggta gggcgccacg atcgcgcgcg tggggcacac gtcgatgcag
     7381 cgcgtgcagg tgccgcagtg ctccgacacc ggctgatcca gcggcagcgg caggtcagtg
     7441 tagatctcgc ccaggaagaa ccaggaaccg gcgctgcggt cgagcaatag cgtgtgcttg
     7501 ccgcgccagc cgagcccggc ctgcgcggcc agc
//
LOCUS       DASPGE010000015         3373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_239765, whole genome shotgun sequence.
ACCESSION   DASPGE010000015 DASPGE010000000
VERSION     DASPGE010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239765"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VD791_00440"
     CDS             <1..463
                     /locus_tag="VD791_00440"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465596.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HXV06472.1"
                     /translation="ETETDLFGEQSVLCGGCVELVKAGFDTLVEAGYAPEMAYFECLH
                     ELKLIVDLMYEGGIGTMNYSISNNAEYGEYVSGPKVIDAGARQRMREILGRIQSGEYA
                     KEFVLENRAGAPVLLSRRRMTAELPIEKVGARLREMMPWIRKNRLVDTSKN"
     gene            468..1124
                     /locus_tag="VD791_00445"
     CDS             468..1124
                     /locus_tag="VD791_00445"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HXV06473.1"
                     /translation="MRPPYPHPIVAREGWPFLAGAVAAAVLVGVLAGWWWSVPVWLAA
                     AFVLQFFRDPPRAVPEDPRAVVSPADGRIVAVEKARDPWLERDALKISVFMNVFNVHS
                     NRSPVDGTVRQRWYHPGAFVNAALDKASLANERNALALRTAEGIDVTCVQIAGLVARR
                     ILCYVEAGAPLVRGQRFGFIRFGSRVDVYLPPEAQAVAALGDKVYAAETVLARLPGRG
                     "
     gene            1153..1902
                     /gene="pssA"
                     /locus_tag="VD791_00450"
     CDS             1153..1902
                     /gene="pssA"
                     /locus_tag="VD791_00450"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HXV06474.1"
                     /translation="MPGLVPPRGGTLRRRGIYLLPNLFTTAALFAGFYAIVQAMNGQY
                     GASAIAIFVAGLLDGLDGRVARLTRTQSEFGAEYDSLSDMVSFGAAPALVLYEWALKD
                     LGRFGWIAAFVYCAGAALRLARFNVMIDVVDKRYFQGLPSPAAAALVAGFVWLAHDNK
                     IPLDAYGLQWVAAFLALYGGLTMVSNAPFYSFKDVNFRKSVPFWAMLLIVLGFVLVSS
                     DPPIVLFLLFCAYGLSGYVLWALGKRPRPRP"
     gene            2105..>3373
                     /locus_tag="VD791_00455"
     CDS             2105..>3373
                     /locus_tag="VD791_00455"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HXV06475.1"
                     /translation="MSKGKLIIFDTTMRDGEQSPGASMTKDEKLRIARALERMRVDVI
                     EAGFPASSNGDFEAVRAIANVVKDSTVAGLCRANDRDIQRGIDALKGAKSWRVHTFIA
                     TSALHMEKKLRMTPDQVFEQAKLSVRYAKNACPDVEFSPEDGSRSDPDFLCRVLEAVI
                     KEGATTINIPDTVGYAVPEQFGEMIRRLRERVPNSDKAVWSVHCHNDLGMAVANSLAA
                     VRIGGARQIECTVNGLGERAGNTSLEEVVMAVRTRKDFFDLETRIDTTQIVPTSRLVS
                     QITGFVVQPNKAVVGANAFAHASGIHQDGVLKARQTYEIMTAEDVGWTTNKIVLGKLS
                     GRTAFKQRLKELGIELDSEDAYNKAFQRFKDLADKKADIFDEDLQALVAQEAAVSADE
                     QWRLVHMSQASGTGERPHADVVLSEHGAERK"
BASE COUNT          537 a         1143 c         1173 g          520 t
ORIGIN      
        1 ggagaccgag accgacctct tcggcgagca gtcggtgctg tgcggcggct gcgtcgagct
       61 ggtgaaggcg ggcttcgaca cgctggtcga ggcgggctac gcgccggaga tggcctactt
      121 cgagtgcctg cacgagctga agctgatcgt cgacctgatg tacgaaggcg gcatcggcac
      181 catgaactac tcgatctcca acaacgccga gtacggcgag tacgtgagcg ggccgaaggt
      241 gatcgacgcg ggcgcgcgcc agcgcatgag ggagatcctc gggcgcatcc agagcggcga
      301 gtacgcgaag gagttcgtgc tcgagaaccg cgccggcgca ccggtgctgc tcagccggcg
      361 gcgcatgacg gccgagctgc cgatcgagaa ggtgggcgcg cggctgcgcg agatgatgcc
      421 ctggatccgc aagaaccgcc tggtcgacac gtcgaagaac tgaaggggtg aggccaccct
      481 acccgcatcc gatcgtcgcg cgcgagggct ggccgttcct cgcgggcgcg gtcgccgccg
      541 ccgtgctggt gggcgtgctg gcggggtggt ggtggtcggt gccggtgtgg ctcgcggcgg
      601 cgttcgtcct gcagttcttc cgcgatccgc cgcgcgcggt gcccgaggac ccgcgcgccg
      661 tggtgtcgcc ggccgacggc cgcatcgtcg ccgtggagaa ggcgcgcgat ccgtggctgg
      721 agcgcgatgc cctcaagatc agcgtgttca tgaacgtgtt caacgtgcac tccaaccgct
      781 cgccggtgga tggcacggtg cgccagcgct ggtaccaccc cggcgccttc gtcaacgcgg
      841 cgctcgacaa ggcgtcgctc gccaacgagc gcaacgcgct cgcgctgcgc acggcggagg
      901 gcatcgacgt cacttgcgtg cagatcgcgg gcctcgtggc gcggcgcatc ctgtgctacg
      961 tggaggccgg cgccccgctg gttcgcggcc agcgcttcgg cttcatccgc ttcggctcgc
     1021 gcgtggacgt ctacctgccg cccgaggcgc aggcggtggc ggcgctgggc gacaaggtgt
     1081 acgccgccga gaccgtgctc gcgaggctgc ccggccgtgg ctgacgcgcc cgagccgcac
     1141 aagcaggact acctgcccgg gctggtgccg ccgcgcggcg gcacgctgcg ccgccgcggc
     1201 atctacctgc tgcccaacct gttcaccacg gcggcgctgt tcgccggctt ctacgccatc
     1261 gtgcaggcca tgaacggcca gtacggcgcg tcggcgatcg ccatcttcgt ggcggggctg
     1321 ctcgacgggc tggacggacg cgtggcgcgc ctgacgcgca cgcagagcga gttcggcgcc
     1381 gagtacgaca gcctgtcgga catggtgtcc ttcggcgccg cgccggcgct ggtgctgtac
     1441 gagtgggcgc tcaaggacct cggccgcttc ggctggatcg ccgcgttcgt ctattgcgcg
     1501 ggcgccgcgc tgcgcctggc gcgcttcaac gtcatgatcg acgtggtcga caagcgctat
     1561 ttccagggac tgccgagccc cgcagccgct gcgctcgtgg ccggcttcgt ctggctggcg
     1621 cacgacaaca agattccgct ggacgcctac ggcctgcagt gggtggccgc gttcctcgcg
     1681 ctgtatggcg ggctgaccat ggtgtccaac gcgccgttct acagcttcaa ggacgtgaac
     1741 ttcaggaaga gcgtgccctt ctgggcgatg ctgctcatcg tgctcgggtt cgtgctggtc
     1801 tcttccgacc cgcccatcgt gttgttcctc ctgttctgcg cctacggcct gtcgggctac
     1861 gtgctgtggg ccctgggcaa gcgtccgcgc ccgcgtcctt gacgctggcg cgcggctggg
     1921 gctatagtcg agcgcatgtc gcaggccatg attctcggca ccatcctcac gctcgcgctc
     1981 ctcgcgctga gcggcctgct gctgcgcccc gcgcgcacaa actaaaaccc cacaattcgg
     2041 cagtgcccgt gccgcccaca aggcggacag ggatcagtcc atccttaccc acgaggacca
     2101 gaccatgagc aaaggcaaat tgatcatctt cgacaccacc atgcgcgacg gcgagcagag
     2161 cccgggtgcg tccatgacca aggacgagaa gctgcgcatc gcccgcgcgc tggagcgcat
     2221 gcgcgtcgac gtgatcgagg ccggttttcc ggcgtcctcc aacggcgact tcgaggcggt
     2281 gcgcgccatc gccaacgtgg tgaaggactc cacggtcgcc ggcctgtgcc gcgccaacga
     2341 ccgcgacatc cagcgcggca tcgacgcgct caagggcgca aagagctggc gcgtccacac
     2401 cttcatcgcc accagcgccc tgcacatgga aaagaagctg cgcatgacgc ccgaccaggt
     2461 gttcgagcag gccaagctct cggtgcgcta cgcgaaaaac gcctgccccg acgtcgagtt
     2521 ctctcccgag gacggcagcc gctccgatcc ggatttcctg tgccgggtgc tggaggcggt
     2581 catcaaggag ggcgccacca ccatcaacat cccggacacc gtgggctacg cggtgccgga
     2641 gcagttcggc gagatgatcc gtcgcttgcg cgagcgcgtc cctaactccg acaaggcggt
     2701 gtggagcgtg cattgccaca acgacctggg catggcggtc gccaactcgc tcgccgccgt
     2761 gaggatcggc ggcgcgcgcc agatcgagtg caccgtcaac ggcctgggcg agcgcgccgg
     2821 caacacctcg ctcgaggaag tggtgatggc ggtgcgcacg cgcaaggact tcttcgacct
     2881 ggagacgcgc atcgacacga cgcagatcgt gccgacctcg cgcctggtgt cgcagatcac
     2941 cggcttcgtg gtgcaaccga acaaggcggt ggtcggcgcc aacgccttcg cccacgcctc
     3001 cggcatccac caggacgggg tgctgaaagc gcgccagacc tacgagatca tgaccgccga
     3061 ggacgtgggc tggaccacca acaagatcgt gctcggcaag ctttccgggc gcaccgcctt
     3121 caagcagcgg ctgaaggagc tcggcatcga gctcgacagc gaggatgctt acaacaaggc
     3181 cttccagcgc ttcaaggacc tggccgacaa gaaagccgac atcttcgacg aggacctgca
     3241 ggccctggtg gcgcaggagg ccgcggtgag cgccgacgag cagtggcggc tggtgcacat
     3301 gagccaggcg agcggcaccg gcgagcggcc acacgccgac gtggtgctca gcgagcacgg
     3361 cgctgagcga aag
//
LOCUS       DASPGE010000016         7669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_473819, whole genome shotgun sequence.
ACCESSION   DASPGE010000016 DASPGE010000000
VERSION     DASPGE010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7669
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473819"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VD791_00460"
     CDS             <1..710
                     /locus_tag="VD791_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXV06476.1"
                     /translation="GSKALPPAYFAYQTLRYLGAQRVHVYHGGFDDWKAAGKPVSTER
                     TALPAVEFHAKLDQRLLVSTQDVVSRLNPGKVQILDVRTAKEFSGDDIRALRGGRIPG
                     AVNVPYEANWIDPDTPRKLQRKQADNKDGMALKPREALRELYAALDPDKETIVYCQSG
                     SRAAESATVLQDLGFKDVKIYDGSWLAWGNAFDAPVENVSWFNVGRVNSLLNQLQGRI
                     DALEAEIEQLKSAARRQ"
     gene            812..1699
                     /gene="glyQ"
                     /locus_tag="VD791_00465"
     CDS             812..1699
                     /gene="glyQ"
                     /locus_tag="VD791_00465"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HXV06477.1"
                     /translation="MNFQDVILTLQRYWGKQGCALLQPYDMEVGAGTFHTATFLRALG
                     PEPWRAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVLKPSPHQIQDLYLASLRELGID
                     PRVNDIRFVEDDWESPTLGAWGLGWEVWLNGMEITQFTYFQEVGSLTCKPVLGEITYG
                     LERLAMYLQGVDNVYDLTWVDGVSYGDVYHQNEVEQSKYNFEHSNTPMLFEHFNQFER
                     EAARLIDAGLPLPGYEMVMKCSHTFNLLDARGAISTTERAAYIARVRALARLVAQAYY
                     DSREALGFPMLKPDKQPAA"
     gene            1704..3788
                     /gene="glyS"
                     /locus_tag="VD791_00470"
     CDS             1704..3788
                     /gene="glyS"
                     /locus_tag="VD791_00470"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HXV06478.1"
                     /translation="MKHTLLVELLTEELPPKALQSLGATFAREVHKGLVEAAFVAADA
                     APPADVLATPRRLAVLAHEVLAVQPDREIERKGPYVAQGLDAHGKPAPALLGFARSCG
                     VEMTALGRGADAKGEHFLFRSVKRGEPIEQHLAAIVTQALKKLPAPSKMMRWGDGDAQ
                     FVRPAHGLIMLLGNRVVAGEVLGLTSGDRTSGHRFLGEAQLRIPSADAYQDTLEKRGK
                     VIASFARRRDAIADALQAAAQREGGAIAEDPALLDEVTALVEFPAVYAGGFDPAFLAV
                     PPECLVLSMKQHQKYFPLHAPATGKLLDRFLIVSNLATDDPRHIVAGNERVLRARLAD
                     AKFFYDQDRKTPLAARVERLGAVVYHNKLGTQLERIQRIRKLASAIADKLHADAAKTE
                     RAAWLCKADLTTDMVGEFPELQGIMGQYYARHDGEDEDVARAIEAHYHPRFAADTLPQ
                     DNVGCAVALADKLDSLAGIYGIGLAPTGDKDPFGLRRQALGVLRILSERALPLDLKEL
                     LQLARLGFAPGMLADSVAVDLHGFMLERLRNYLRERDYAIDEIEAVVGQNPTRIDLVV
                     PRLKAVQAFRALPEAQALASANKRIRNILKKTTVPQAHADPALLTEGAERNLHDATAR
                     LLPAVRSLVDNEDYTEALRVLAGVRKEVDTFFDEVMVMTDEPLVRNNRLALLAQLEAL
                     LNQVADISRLAQ"
     gene            3785..4324
                     /gene="gmhB"
                     /locus_tag="VD791_00475"
     CDS             3785..4324
                     /gene="gmhB"
                     /locus_tag="VD791_00475"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HXV06479.1"
                     /translation="MKLVILDRDGVINTDSDQFIKTPDEWKPIPGSLEAIARLTHSGF
                     RVVVCSNQSGVGRGLLDMAALNAINDKMYKALAPFGGRIDALFYCPHPAEANCACRKP
                     RPGMFQDIAKRFNVTLSGVPSVGDSLRDMQASAAAGCQPLLVLTGKGRKTLAAGGLPE
                     GTQVFDDLSAAVRAIIAKP"
     gene            4321..5052
                     /locus_tag="VD791_00480"
     CDS             4321..5052
                     /locus_tag="VD791_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXV06480.1"
                     /translation="MSRAISSALRSALFLLLQAALTIVWSLLSLLTFPFSPLTRYRFI
                     TLWSRMVIRLAGAICGIRYEVRGLEHLPARPGIILSKHQSAWETLAFQVFLPPQVWVL
                     KRSLLRIPFFGWGLAMMNPIAIDRAAGMAALKQTLEQGRERLAQGFWIVIFPEGHRFP
                     PGEKGTYHPGGAWLAIRTGAPVVPIALNSGYLWRRDAFVKLPGKITVSIGRPIETGST
                     KAEALMRRVEDWIETETARLGDPRS"
     gene            5073..5792
                     /locus_tag="VD791_00485"
     CDS             5073..5792
                     /locus_tag="VD791_00485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HXV06481.1"
                     /translation="MLTEPRTVRLGERDLPYLLKRSAQRRRLVLLVDERGLVVHVPWR
                     ASERHIAQAIAEAQSWILRKLAEWERKKPRQRSWTGGEALDFLGRQLTLQLHARDGPA
                     IAQLRDGSALELSLAAPHTPERVRNAVVSWYRRHAGSHFAERVQHFCARLDVPAPCVR
                     LSSARTRWGSCNADGEIRLNWRLMQAAGPVIDYVVAHEVAHLKVMSHSTRFWRTVQRL
                     CPDYEAAKAELSAMSLHFMAL"
     gene            5876..6121
                     /locus_tag="VD791_00490"
     CDS             5876..6121
                     /locus_tag="VD791_00490"
                     /inference="COORDINATES: protein motif:HMM:NF025012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06482.1"
                     /translation="MHRTAILLLIAQACASGCGLKGPLYIPTAEEERELAERKRALEE
                     RERREREEQPASTNPAPPQASSTQPPARVEPGSVPTN"
     gene            6128..7390
                     /gene="lysA"
                     /locus_tag="VD791_00495"
     CDS             6128..7390
                     /gene="lysA"
                     /locus_tag="VD791_00495"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HXV06483.1"
                     /translation="MPAALPQSPFIGYRAGALHVEDVPLAQVAQQHGTPCFVYSRAHL
                     TGAFHAFDRPFGTQPHLICYAAKANSSLAILNLLARLGSGFDIVSGGELERVLAAGGE
                     AGKVVFSGVGKRADEIELGLAKGVLCFNVESESELMRISAIAAKSRRTANISLRVNPD
                     VDPRTHPYISTGRAENKFGVPFDEAFALYGKASALPGLAIAGIDYHIGSQMTEVAPFL
                     DALDRLLRLVHRLAGAGIPLRHLDLGGGFGIRYRDESPPEPAAYIEAALERLGSVEQT
                     VILEPGRALVGNAGVLLTRVEYLKHGAHNFAVVDAAMNDLIRPALYDAFHEIVPVVAR
                     QGARRRYEVVGPICESADFLGHDRELALAEGDLLAVLSAGAYGMSMSSNYNTRPRAAE
                     VMVDGATVHLVRERERVAQLFALERLLP"
BASE COUNT         1217 a         2694 c         2625 g         1133 t
ORIGIN      
        1 acggcagcaa ggcgctgccg ccggcgtatt tcgcctacca gacgctgcgc tacctgggcg
       61 cgcaacgcgt gcacgtgtat cacggcggct tcgacgactg gaaggcggcg ggcaaaccgg
      121 tgagcaccga gcgcaccgcc ctgccggcgg tggagttcca tgcgaagctc gaccagcggc
      181 tgctcgtctc cacgcaggac gtggtctcgc gcctgaaccc gggcaaggtg cagatactgg
      241 acgtgcgcac ggccaaggag ttcagcggcg acgatatccg cgcgctgcgc ggcggccgca
      301 tcccgggcgc ggtcaacgtc ccctacgaag ccaactggat cgacccggac acgccgcgca
      361 agctgcagcg caagcaggcc gacaacaagg acggcatggc gctcaagccg cgcgaggcgt
      421 tgcgcgaact gtatgccgcg ctcgaccccg acaaggagac catcgtctac tgccagagcg
      481 gcagccgcgc cgccgagagc gcgaccgtgc tgcaggatct aggcttcaag gacgtcaaga
      541 tctacgacgg ctcctggctc gcctggggca acgccttcga cgcgccggtg gagaacgtca
      601 gctggttcaa cgtcgggcgc gtgaacagcc tgctcaacca gctgcaggga cgcatcgacg
      661 cgctggaggc ggagatcgag cagctcaagt cggctgcccg gcggcaatag cggcggccgc
      721 tcgcgcgccg ccgtcatgcg cgcggcggga aacacagtag aatccgtgtt ttccgtcgcg
      781 ccaagtgcgc gccggcccgg tcgagagcgg catgaacttc caggatgtga tcctcacgct
      841 ccagcgttac tggggcaagc aaggctgcgc gctgctgcag ccctatgaca tggaggtggg
      901 tgcgggcacc tttcacaccg cgaccttctt acgcgcgctc ggccccgagc cctggcgcgc
      961 cgcctatgtg cagccctcgc gccgccccaa ggacggccgc tacggcgaga accccaatcg
     1021 cctgcagcac tactaccagt accaggtggt gctcaagccc tcgccccacc agatccagga
     1081 cctgtacctg gcgtcgctgc gcgagctggg catcgacccg cgcgtgaacg atatccgctt
     1141 cgtcgaggac gactgggagt cgcccacgct gggcgcctgg gggctgggct gggaggtgtg
     1201 gctgaacggc atggagatca cccagttcac ctacttccag gaggtgggca gcctgacctg
     1261 caagccggtg ctgggcgaga tcacctacgg cctggagcgg ctggccatgt acctgcaggg
     1321 cgtggacaac gtctacgacc tgacctgggt ggacggcgtg agctacggcg acgtctatca
     1381 ccagaacgag gtggagcagt cgaagtacaa cttcgagcac tccaacacgc cgatgctgtt
     1441 cgagcatttc aaccagttcg agcgcgaagc cgcgcgcctg atcgacgccg ggctgccgct
     1501 accgggctac gagatggtga tgaagtgctc gcacaccttc aacctgctcg acgcgcgcgg
     1561 cgccatctcg accaccgagc gcgccgccta catcgcccgc gtgcgcgcgc tggcgcgcct
     1621 ggtggcgcag gcctattacg actcgcgcga agcgctgggc ttcccgatgc tgaaacccga
     1681 caagcagccc gccgcgtgaa gtcatgaaac acacgctgct ggtcgagctg ctcaccgagg
     1741 agctgccccc gaaggcgttg cagtcgctgg gcgcgacctt cgcgcgcgag gttcacaagg
     1801 ggctggtgga ggccgccttc gtcgccgcgg acgcggcgcc gcccgcggat gttctcgcga
     1861 cgccgcggcg cctggcggtg ctggcgcacg aggtcctcgc cgtgcaaccc gaccgcgaga
     1921 tcgagcgcaa gggcccgtac gtggcgcaag gcctcgacgc gcacggcaag ccggcgcccg
     1981 cgctgctggg cttcgcccgc agttgcggcg ttgagatgac ggcgctcggc cgcggcgcgg
     2041 acgccaaggg cgagcacttt ctctttcggt cggtcaagcg cggcgagccg atcgagcagc
     2101 acctcgccgc gatcgtcacc caagccctga agaagctgcc cgcccccagc aagatgatgc
     2161 gctggggcga cggcgacgcc cagttcgtgc ggccggcgca cgggctgatc atgctgctcg
     2221 gcaaccgcgt cgtggccggc gaggtgctgg gactcacgag cggcgatcgc accagcggcc
     2281 accgttttct gggcgaggcg cagctgcgca tccccagcgc cgacgcctat caggacacgc
     2341 tggagaagcg cggcaaggtg atcgcgagct tcgcgcggcg gcgcgacgcg atcgcggacg
     2401 cgctgcaagc ggcggcccaa cgcgagggcg gcgccatcgc cgaggacccg gcgctgctcg
     2461 acgaggtgac cgcgctggtg gagtttccgg cggtgtacgc cggcggcttc gatcccgcct
     2521 ttctcgcggt gccgccggaa tgcctagtgc tgtcgatgaa gcagcaccag aagtactttc
     2581 cgctgcacgc gccggccacg ggcaagctgc tcgaccgctt cctgatcgtg agcaatctcg
     2641 ctaccgacga tccgcgccac atcgtcgccg gcaacgagcg cgtgctgcgc gcgcgcctgg
     2701 ccgacgccaa gttcttctac gaccaggacc gcaagacacc gctcgccgcg cgcgtcgagc
     2761 gcctgggcgc ggtcgtgtac cacaacaagc tcggcaccca gctcgagcgc atccagcgca
     2821 tccgcaagct cgccagcgcc atcgccgaca agctgcacgc ggacgcggcc aagaccgagc
     2881 gcgccgcctg gctgtgcaag gccgacctca ccaccgacat ggtcggcgag ttccccgagc
     2941 tgcagggcat catggggcag tactacgccc gccacgacgg cgaggacgag gacgtggcgc
     3001 gcgcgatcga ggcgcactat cacccgcgct tcgccgccga cacgctgccg caggacaacg
     3061 tcggctgcgc ggtggcgctg gccgacaagc tcgacagttt ggcgggcatc tacggcatcg
     3121 gcctcgctcc caccggcgat aaggacccgt tcggcctgcg ccggcaggcg ctgggtgtgc
     3181 tgcgcatcct gtccgagcgc gcgctgccgc tggacctgaa ggagctgctg cagctcgcgc
     3241 ggctcggctt cgcgcccggc atgctggccg acagcgtggc cgtcgacttg cacggtttca
     3301 tgctggagcg gctgcgcaac tacctgcgcg agcgcgatta cgcgatcgac gagatcgagg
     3361 cggtggtggg acagaacccc acgcgcatcg acctggtggt gccgcgcctg aaggcggtgc
     3421 aggcgttccg cgcactgccc gaggcgcagg cgctggcgag cgccaacaag cgcatccgca
     3481 acatcctgaa gaagaccacc gtgccgcagg cgcacgccga tcccgccctg ctcaccgaag
     3541 gcgccgagcg caacctgcac gacgcgaccg cgcgcctgct gcccgcggtg cgctcgctgg
     3601 tggacaacga ggactacacc gaggcgctgc gcgtgcttgc cggcgtgcgc aaggaggtgg
     3661 atactttctt cgacgaggtc atggtcatga ccgacgagcc gctggtccgc aacaaccgcc
     3721 tggcgctgct ggcgcagctg gaggcgctgc tgaaccaggt ggcggacatc tcgcggctgg
     3781 cgcaatgaag ctggtgatac tggatcgcga cggtgtgatc aataccgaca gcgatcagtt
     3841 catcaagact cccgacgagt ggaagccgat tccgggcagc ctggaggcga tcgcgcgcct
     3901 gacgcactcc gggttccgcg tcgtggtgtg ctccaaccag tccggcgtgg ggcgcgggct
     3961 gctcgacatg gccgctttga acgccatcaa cgacaagatg tacaaggcgc tcgccccttt
     4021 cggcgggcgc atcgacgcgc tgttctactg cccgcatccg gccgaggcga actgcgcctg
     4081 ccgcaagccc cggccgggca tgttccagga catcgccaag cgcttcaacg tcacgctcag
     4141 cggcgtgccc agcgtgggcg attccctgcg cgacatgcag gcctcggcgg cggccggctg
     4201 ccagccgctc ctggtgctca ccggcaaggg ccgcaagacc ctggccgcgg gcggcctgcc
     4261 cgagggcacg caggtgttcg acgacctgtc cgccgcggtg cgcgcgatca tagccaagcc
     4321 atgagccggg ccatctcgtc cgccctgcgc tcggccctgt tcctcctgct gcaagccgct
     4381 ctcaccatcg tctggtcgct gctgtcgctg ctgacctttc cgttcagccc gctgacgcgc
     4441 taccgcttca tcacgctctg gtcgcgcatg gtgatccggc tcgctggagc gatctgcggc
     4501 atccgctacg aggtgcgcgg gctggagcat ttgccggctc gtcccggcat catcctgtcc
     4561 aagcatcaat cggcgtggga aacgctcgcc ttccaggtgt tcctgccgcc gcaggtgtgg
     4621 gtgctcaagc gcagtctgct gcgcattccg ttcttcggct ggggcctggc gatgatgaac
     4681 ccgatcgcca tcgaccgcgc cgcgggcatg gcagcgctca agcagaccct ggagcaggga
     4741 cgcgagcgcc tggcgcaggg cttctggatc gtgatctttc ccgaaggcca ccgcttcccg
     4801 ccgggggaga aaggcaccta tcatccgggc ggcgcctggc tcgccatccg gaccggtgcg
     4861 ccggtagtgc cgatcgcgct gaactcgggg tatctttggc gccgcgacgc cttcgtcaag
     4921 ctccccggta agatcacggt gagcatcggc cggccgatcg aaaccggctc gacgaaagcc
     4981 gaagcgctca tgcggagagt ggaagactgg atcgaaaccg aaaccgcccg actgggggat
     5041 ccgcgcagct agatctgttc gcggagcccg ccatgctcac cgagccgcgc acggtgcgtc
     5101 tcggcgagcg cgacctgccc tacctgctca agcgcagcgc gcagcgccgg cgcctggtgc
     5161 tgctggtgga cgagcgcggc ctagtggtgc acgtgccgtg gcgtgcgtcc gagcgccaca
     5221 tcgcgcaggc catcgccgag gcgcagtcct ggatcctgcg caagctcgcg gagtgggaac
     5281 gcaagaaacc gcgccagcgc agctggaccg gcggcgaggc gctcgacttc ctggggcggc
     5341 agctcacttt gcagcttcac gcccgcgacg gccccgcgat cgcgcaactg cgcgacggca
     5401 gcgcgctgga gctgtcgctg gccgcgccgc acacgccgga gcgcgtgcgc aacgcggtgg
     5461 tgagctggta ccgccgccac gcgggcagcc acttcgccga gcgcgtgcaa catttctgcg
     5521 cgcggctgga cgtgccggcg ccgtgcgtgc ggctgtcgag cgcccgcacg cgctggggga
     5581 gctgcaacgc cgacggcgaa atccgcctca actggcgcct gatgcaggcc gccggccccg
     5641 tgatcgacta cgtggtggcg cacgaggtgg cgcacctcaa ggtgatgagc cattcgacgc
     5701 gcttctggcg caccgtccag cgcctgtgcc cggactacga ggcggccaag gccgagctga
     5761 gcgcgatgag cctgcacttc atggcgttat gactccctct ccccttgcgg gagagggact
     5821 tgtctgtctg ctcgggagag gggagcctcg tgctagactc gcgcggatgc gcgcgttgca
     5881 tcgtaccgcc atcctcctgt tgatcgccca ggcttgcgcc tcgggctgcg gcctgaaggg
     5941 cccgctttac atccccaccg ccgaggagga acgcgagctg gccgaacgca agcgcgcgct
     6001 ggaggagcgc gagcgccggg agcgcgagga acagccggct tcgaccaacc ccgcgccgcc
     6061 gcaggcgtcg agcacgcagc caccggcacg ggtcgaaccc ggcagcgtcc cgaccaactg
     6121 acgtttcatg cctgctgctc tgccccagtc gccgttcatc ggctaccgcg ccggcgcgtt
     6181 gcacgtcgag gatgtgccgc tcgcgcaagt ggcgcagcag cacggcacgc cgtgcttcgt
     6241 gtattcgcgc gcccatctca ccggcgcctt tcacgcattc gaccggccct tcggcacgca
     6301 gccgcatctc atctgctatg ccgcgaaagc caactccagt ctggcgatcc tcaacctgct
     6361 cgcccggctg gggagcggtt tcgacatcgt ttcgggcggc gagctggagc gggtgctggc
     6421 cgccggcggc gaggccggca aggtggtgtt ctcgggcgtg ggcaagcgcg cggacgagat
     6481 cgagctgggg ctcgccaagg gtgtgctgtg cttcaacgtc gagtccgaaa gcgagctgat
     6541 gcgcatcagc gcgatcgcgg cgaagtcccg gcgcacggcg aacatcagcc tgcgcgtcaa
     6601 cccggacgtc gatccccgca cccatcccta catctcgacc gggcgcgcgg agaacaagtt
     6661 cggggtgccc ttcgacgagg cgttcgcgct gtacggcaag gccagcgcgc tgccggggct
     6721 ggcgatcgcc ggcatcgact accacatcgg ctcgcagatg accgaggtcg cgccgttcct
     6781 ggacgcgctc gaccgcctgc tgcgcctggt gcaccgcctg gccggcgcgg gcatcccgct
     6841 gcggcacctg gacctcggcg gcggtttcgg catccgctac cgcgacgagt cgccgcccga
     6901 gccggccgcc tacatcgagg ccgcgctcga gcggctgggt agcgtcgagc aaaccgtcat
     6961 cctggagccc ggccgcgcgc tggtgggaaa cgccggcgtg ctgctgacgc gcgtggaata
     7021 tctgaagcac ggcgcgcaca acttcgccgt cgtggatgcg gcgatgaacg acctgattcg
     7081 gccggcgctg tacgacgcgt tccacgaaat cgtgccggtg gtcgcgcggc agggcgcgcg
     7141 ccggcgctac gaggtggtgg gcccgatctg cgagagcgcc gacttcctcg gccacgaccg
     7201 cgagctggcg ctcgccgagg gtgatctcct ggcggtgctc tccgcgggcg cctacggcat
     7261 gagcatgagc tcgaactaca acacccgccc gcgcgccgcc gaggtgatgg tggacggcgc
     7321 caccgttcac ctggtacggg agcgcgagcg ggtggcgcaa cttttcgcgc tggagcggct
     7381 cttaccctag cctgaagttc cccctgggcc ctacggggaa gcgctagaat ctcgaagttg
     7441 cgccgcatca gcggggcaca accgacagca ggcgcagata gtcgagtgca caccgatcga
     7501 atcgctcgga tgcgaggcgc atcctcgcaa tctccggccg gcgcaacgcc gccgcgctcc
     7561 tgtgctgcct tgctgctgct tgcgctcgcc gctgccgggt gcagcgacag caagagcgcc
     7621 gccgaaaaga aggccgccgc gaccccgccc gctccggtca ccgtcgccc
//
LOCUS       DASPGE010000017         2780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1294071, whole genome shotgun sequence.
ACCESSION   DASPGE010000017 DASPGE010000000
VERSION     DASPGE010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294071"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="VD791_00500"
     CDS             complement(<1..1050)
                     /locus_tag="VD791_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV06484.1"
                     /translation="MSLLLAPGLAGAAAGTPQTVVNLLDYISVEYPAFVRDGRVLDAD
                     EYAEQVEFSSQVRDLIAALPATAQRAALVARAAELSRLVQEKADAAQVVALARDVQRG
                     VIDAYAVAVTPRQPPDLATAATLYAAHCGQCHGALGDGAGVAAAGLDPAPTDFRDAER
                     ASARSVYGLYNTITLGVAGTAMAGFGQLSEEQRWALAFHVSGFAADDALRARGAAAWK
                     RAAGRDRFPDLAAVATATPSEVRGAGPEAEAILAFLRSQPSAAAAAPGSPIDFSIATL
                     DRSREAWRAGRTDEAYQLAVSAYLEGFELAEPGLASADSQLTKRTEQALMAYRNAIHA
                     GATAQQLETAHAAAIA"
     gene            1326..1655
                     /locus_tag="VD791_00505"
     CDS             1326..1655
                     /locus_tag="VD791_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HXV06485.1"
                     /translation="MNATLVGADRLGNIPAVLRSRGIAVLQHISGRTAGAQRRLPCLR
                     RGTQLLILFTDFINHNAMQGFRRAAQEQGIPVLACRRSSSCLLECLERNGLCRACADL
                     SVAGTIP"
     gene            1739..1936
                     /locus_tag="VD791_00510"
     CDS             1739..1936
                     /locus_tag="VD791_00510"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXV06486.1"
                     /translation="MFVCVCNGVTERQIREAIDRGARSLPELTAALGVAAGCGACADH
                     TRRMLEGFVPARELDALPCAA"
     gene            2101..2565
                     /gene="bfr"
                     /locus_tag="VD791_00515"
     CDS             2101..2565
                     /gene="bfr"
                     /locus_tag="VD791_00515"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HXV06487.1"
                     /translation="MKGDSKVLEYLNKVLTNELTAINQYFLHARMFRNWGFKKLDEYE
                     YHESLDEMKHADQLINRILFLEGLPNLQQLGKLLIGESVPEALRCDLGLEMQALPLLR
                     EAIAHCEAVSDYISRELLEDILDSEEEHVDWLETQLELIDRTGLQNYLQSQM"
BASE COUNT          439 a          942 c          973 g          426 t
ORIGIN      
        1 ggcgatggcg gccgcatgcg ccgtctccag ctgctgtgcc gtggcgccgg cgtggatggc
       61 attgcggtag gccatcagcg cctgctcggt gcgcttggtg agctgcgaat ccgcgctggc
      121 gagcccgggc tcggccagct cgaagccttc gaggtaggcc gagacggcga gctggtaggc
      181 ctcgtccgtg cgtcccgcgc gccacgcttc gcggctgcgg tccagggtgg cgatgctgaa
      241 gtcgatgggc gagccgggtg cagcggccgc ggccgaaggc tggctgcgca ggaatgcgag
      301 gatcgcttcg gcctcagggc ctgcgccgcg cacctcggac ggcgtggcgg tcgccaccgc
      361 cgccaggtcg gggaagcgat cgcggcccgc ggcccgcttc caggcggccg cgccgcgcgc
      421 acgcagggcg tcgtcagcgg cgaagccgct cacatggaac gccagcgccc agcgctgctc
      481 ctcggacagc tggccgaatc cggccatggc ggtgccggcc acgcccaggg tgatggtgtt
      541 gtacagcccg tacacgctgc gtgccgaagc gcgttcggcg tcgcggaagt cggtgggcgc
      601 cgggtcgagg ccggcggcgg ccacacccgc gccatcgccc agtgcgccgt ggcattgccc
      661 gcaatgggcg gcgtacaggg tggcggcggt tgccaggtcg ggcggctgcc gcggcgtcac
      721 ggccaccgca taggcatcga tcacgccgcg ctggacatcg cgcgccagcg ccaccacctg
      781 tgcggcgtcg gctttctctt gcaccaggcg gctcaactcg gcggcgcgcg ccaccagcgc
      841 ggcgcgctgc gcggttgccg gcagcgccgc gatgaggtcg cgcacctgcg acgagaactc
      901 cacctgctcc gcgtactcgt cggcgtcgag cacgcggccg tcgcggacga aggccggata
      961 ctccaccgag atgtagtcga gcagattgac cacggtctgg ggcgtgccgg cggcggcgcc
     1021 cgccaggcca ggcgcaagca gcagcgacac gagcgcgagt aagacccgca ggggcacgtt
     1081 tcggaagagg cggcaggcgt ttggaatgcg aattatatgc attacgccgg tctcacgaaa
     1141 gatagcggtt accgagctcc agttagcggc ggcagacgct tggaacgcct tggaaggata
     1201 ccgcgcgcgg ccttcgcgac acgccgtgcc cgcctctcag ctcttccgcc tgcgtgggtg
     1261 cgttctcttc ccaacagaga tgagaatgca tatcatttgt gttgaatcgg cattgaggag
     1321 gttcgatgaa cgcaaccctg gtcggggcgg atcgcctggg caatatcccc gctgtcctgc
     1381 gcagccgcgg gattgccgtg ctgcagcaca tcagcggacg gactgcgggc gctcagcggc
     1441 gcctgccgtg cctccggcgt ggaacgcaac tgcttattct gtttacggat ttcatcaatc
     1501 ataacgcgat gcagggcttc cggcgggcgg cgcaggagca aggcattccg gtgctggcct
     1561 gccgccgttc gagcagctgc ctgctcgaat gcctggagcg caacggcctg tgcagggcgt
     1621 gcgccgatct cagcgtcgcc gggaccatcc cctgagcagc ggtgaggttg tgcatcgcgc
     1681 agttgacact cattctcatt gcgttataga gttgcgcggc ctgggaggga aaaacgcctt
     1741 gttcgtctgc gtctgcaacg gggtcacgga acgccagatc cgcgaggcca tcgaccgcgg
     1801 agccagatcg ctgccggaac tgaccgccgc gctcggcgtc gcggcggggt gcggcgcttg
     1861 cgctgatcac acgcgccgca tgctggaggg tttcgtcccc gcgcgcgagc tcgacgccct
     1921 gccgtgcgcc gcctaggccg taacatctag cggcccgcca ctcccgcttc cctcggatac
     1981 gcgcgcggtg cgcgtgccgc catcgcgctg atgcgaaacc gtcgcgcagc gcgccgccga
     2041 cccgacctgc gcctcgaggc cggctataat cccccgctgt ttctcgccga gggagcgcag
     2101 atgaagggtg acagcaaggt gctcgagtac ctgaacaagg tgctgaccaa cgagctgacc
     2161 gcgatcaacc agtacttcct gcacgcgcgc atgttccgca actggggatt caagaagctc
     2221 gacgagtacg agtaccacga gtcgctcgac gagatgaagc acgcggacca gctgatcaac
     2281 cgcatcctgt tcctggaagg gctgccgaac ttgcagcagc tgggcaagct gctgatcggc
     2341 gagagcgtgc cggaggcgct gcgctgcgac ctcggcctgg agatgcaggc gctgccgctg
     2401 ctgcgcgaag ccatcgccca ctgcgaagcg gtgagcgact acatcagccg cgagctgctg
     2461 gaggacattc tcgacagcga ggaggagcac gtggactggt tggaaaccca gctcgagctg
     2521 atcgaccgca ccggcttgca gaattacctg caatcgcaga tgtagcgctg caggtccagc
     2581 cttggccgtg tgcccgccca cgacgattca gcgcctgctc gcgctcgcag gctgcgtggc
     2641 gctgctcgcg ggctgcgcta cggctcccaa gtcgccgccg gacaccccgg cgccgccgca
     2701 gccgccggcg acgacgtccg cggcgactcc cgcaacgacc cccgggacga cccctgggac
     2761 aacccctggg acaaccgagg
//
LOCUS       DASPGE010000018         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1060019, whole genome shotgun sequence.
ACCESSION   DASPGE010000018 DASPGE010000000
VERSION     DASPGE010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060019"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VD791_00520"
     CDS             <1..330
                     /locus_tag="VD791_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HXV06488.1"
                     /translation="YFVGMLGFAPGFPYLAGMDQRLIAARRATPRPRVPAGSVAIGGE
                     HTGIYPFASPGGWHLIGRTPLTLFALDRDPPSLLQAGDRVRFVPIAPDEFQRTAEFER
                     TAEERRA"
     gene            397..1401
                     /locus_tag="VD791_00525"
     CDS             397..1401
                     /locus_tag="VD791_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HXV06489.1"
                     /translation="MALKVLRAGILSTLQDLGRWGYQRHGVPVGGVMDEVSHRLANLL
                     AGNPEDQATVEMTLVGPGFALTRDALIAVCGGEFEARINGEALPRARPVLARAGSTLD
                     FGVCRRGCRAYLAVAGGFAVEPVLGSRSTYLRGGFGGWRGRALQRGDLLPTAEPDPAR
                     YPSLHRKLGERRAPMAYPGWSVTERVELLAPGPYLLRFVPGRHWEAFPQPVRERFLGA
                     EYRVGSNSDRQGYRLAGPLLVPGAPLEIISGGVTFGTIQVPPDGEPIVLMANRQTTGG
                     YPRLGEVAAVDLPLLAQLPPGAPVRFQQVEMATAQSLLIERERDLARTREGIALQSRR
                     "
     gene            1555..>2014
                     /locus_tag="VD791_00530"
     CDS             1555..>2014
                     /locus_tag="VD791_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06490.1"
                     /translation="MKTLIAAALLLLAATSNAGAAGTLADVTVYDRIAQRSLPVHWSE
                     GGAWVAGAPGSEYSIRVRNRSAADLLAVISVDGVNAVTGETASPDQSGYIVPAYGSVE
                     IKGWRKSMRRVASFYFTDLGDSYAARTGRPDDVGVIGVALFKRKPAPDLHF"
BASE COUNT          257 a          733 c          717 g          307 t
ORIGIN      
        1 tacttcgtcg ggatgctggg cttcgcgccg ggcttccctt atctcgccgg catggaccag
       61 cgcctgatcg ctgcgcgccg cgccaccccg cggccgcgcg tgcccgcggg cagcgtggcc
      121 atcggcggcg agcacaccgg catctatccg ttcgcctcgc ccggcggctg gcacctgatc
      181 gggcgcacgc cactcacgct gttcgcgctc gatcgcgatc cgcccagcct cctgcaggcg
      241 ggcgaccgcg tgcgcttcgt gccgatcgcg cctgacgaat tccagcgcac ggcggagttc
      301 gaacgcaccg cggaggaacg ccgtgcctga tcgtcgtgcc cccgatcgtt gcgtccccga
      361 tccttgcgac gtggcgcaag cacagaggga gccgaggtgg cgttgaaggt gctgcgcgcg
      421 gggatcctgt ccacgctgca ggatctcggg cgctggggct accagcgcca cggcgtgccg
      481 gtgggcggcg tgatggacga agtgtcgcat cgcctcgcca acctgctggc cggcaacccc
      541 gaggatcagg cgacggtgga gatgaccctg gtgggaccgg gcttcgccct cacgcgcgat
      601 gcgctcatcg cggtgtgcgg gggcgagttc gaggcgcgca tcaacggcga ggcgttgccg
      661 cgcgcgcgcc cggtgctggc gcgcgccggc agcacgctgg acttcggcgt gtgccggcgc
      721 ggctgccggg cttacctggc ggtggccggc ggcttcgcgg tggagccggt gctgggcagc
      781 cgcagcacgt atctgcgcgg cggtttcggc ggctggcgcg ggcgcgcgct gcagcgcggc
      841 gatctgctcc ccacggccga gcccgacccg gcgcgctatc ccagcctgca tcgcaagctg
      901 ggtgagcggc gcgcgccgat ggcctatccc ggctggtcgg tcaccgagcg cgtcgagctg
      961 ctcgcacccg ggccgtacct gctgcgtttc gtgcccggcc gtcactggga ggccttcccg
     1021 caaccggtgc gcgagcgctt tctgggcgcc gagtaccgcg tcggcagcaa ctccgaccgc
     1081 cagggctatc ggttggcggg gccgctgctc gttcccgggg cgccgctcga gatcatctcc
     1141 ggcggcgtga ccttcggcac catccaggtg ccgccggacg gcgagccgat cgtgctgatg
     1201 gccaaccgcc agaccaccgg cggctacccg cgcctgggcg aggtggcggc cgtggatctg
     1261 cccctgctcg cgcagcttcc gcccggcgcg ccggtgcgct tccagcaggt ggaaatggcc
     1321 accgcccagt cgctgctgat cgagcgcgag cgcgacctgg cgcgcacgcg cgaagggatc
     1381 gcactacagt cgcgccgatg aagctagacc tgaactgcga cacaggcgag cactgacggc
     1441 gccccggttt cacccccggc tcaacccccg tcggctcaac cccccgggtt aaccctttag
     1501 cgctgcggcc gcgtttaccg tctctgtgat ccatctttca cagaggagaa acgcatgaag
     1561 accctgatcg ccgcagcgct gctgctgctc gcggctacct cgaacgccgg cgcggcgggc
     1621 acgctcgccg acgtgaccgt ctacgaccgc atcgcgcagc gttccctgcc ggtgcactgg
     1681 agcgaaggcg gcgcctgggt ggcgggcgcg ccgggcagcg agtattcgat ccgcgtccgc
     1741 aaccgcagcg ctgcggacct gctggcggtg atctcggtgg acggcgtgaa cgccgtcacc
     1801 ggcgagaccg cgtcgcccga ccagagcggc tacatcgtgc cggcttacgg gtcggtcgag
     1861 atcaagggct ggcgcaagag catgcgtcgc gtcgcgtcgt tctacttcac cgacctcggc
     1921 gattcctacg cggcgcgcac cggccgtccg gacgacgtcg gcgtgatcgg cgtggcgctg
     1981 ttcaagcgca agcccgcgcc cgacctgcac ttcc
//
LOCUS       DASPGE010000019         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_942487, whole genome shotgun sequence.
ACCESSION   DASPGE010000019 DASPGE010000000
VERSION     DASPGE010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942487"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /gene="rimP"
                     /locus_tag="VD791_00535"
     CDS             complement(<1..350)
                     /gene="rimP"
                     /locus_tag="VD791_00535"
                     /inference="COORDINATES: protein motif:HMM:NF000929.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HXV06491.1"
                     /translation="MEPILAGMGLELVDMQVSNRGRFLRIFMDKPDGITVEDCAAVSR
                     HLSRVLEVEGVDYDRLEVSSPGLDRPLRKAADFARFAGQKVDVRMRLADANGRRRYIG
                     RLKGIEGATATVEV"
     gene            complement(421..1209)
                     /locus_tag="VD791_00540"
     CDS             complement(421..1209)
                     /locus_tag="VD791_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HXV06492.1"
                     /translation="MSPRRRPLSPKPPTNPPQAERLQKLLAAAGFGSRREIEGWIAAG
                     RLRVNGVVTKLGERARPGDVIELDGKVLALKATGARPRVLLYHKPDGEMVTRSDPEGR
                     PTVFQRLPPIPGGKWVAVGRLDINTAGLLLLTDCGELANRLMHPRYEVEREYAVRVLG
                     ALHPEERAKLLSGVTLDDGIGRFDRLEPSGAAEGANRWYRVVLREGRNREVRRLFEAL
                     GRKVSRLLRVRYGPVELPRDLKPGRWIELDEERVAEIVKTAKSG"
     gene            complement(1229..1735)
                     /gene="scpB"
                     /locus_tag="VD791_00545"
                     /pseudo
     CDS             complement(1229..1735)
                     /gene="scpB"
                     /locus_tag="VD791_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516749.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
     gene            complement(1722..>2174)
                     /locus_tag="VD791_00550"
     CDS             complement(1722..>2174)
                     /locus_tag="VD791_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HXV06493.1"
                     /translation="DPRAELVRRLLEYERIKKAAQQLDQLPQLGRDVLAVSVWVERVV
                     TEQLPGVDPQDLAEAWRTLLHRARMSAHHRVSREELSVREHMSGILRRLQERRVLEFG
                     ELFDPARGVAVLVVTLLAVLELARESLLELTQSESFAPIYVKLAHAAH"
BASE COUNT          319 a          827 c          705 g          323 t
ORIGIN      
        1 tccacctcca cggtcgccgt ggccccctcg atgcccttca accggccgat gtagcgccgg
       61 cggccgttcg cgtcggccag ccgcatcctg acgtcgactt tctgccccgc gaagcgcgca
      121 aaatccgcgg ccttgcgcag cggccggtcc agccccggcg aagacacctc cagccggtcg
      181 tagtcgacgc cctcgacctc cagcacgcgg gacaggtggc ggctcacggc cgcacagtcc
      241 tccaccgtga tgccgtccgg cttgtccatg aaaatgcgca ggaaacggcc ccggttggac
      301 acctgcatgt ccaccagttc caaccccatc ccggcaagga tgggttccac gattccttcc
      361 agcgcgctcg ccacaaacaa aaaatgggcc gaagcccacc cgcatgaaac ggcttgaagt
      421 ttaaccgctt tttgccgttt tcacaatctc cgcgacgcgt tcctcgtcga gctcgatcca
      481 gcgccccggc ttgaggtcgc gcggcagttc caccgggcca tagcgcacgc gcagaagccg
      541 gctgaccttg cgcccgaggg cctcgaacag ccggcgcact tcccgattgc gcccctcccg
      601 cagcacgacc cggtaccagc ggttggcgcc ttcggccgcg cccgagggct ccagccggtc
      661 gaagcgcccg atgccgtcgt ccagcgtcac gccggacagc agcttggcgc gctcctcggg
      721 atgcaaagcc ccgagcacgc gcaccgcgta ttcgcgctcg acctcgtacc gcgggtgcat
      781 gagccggttg gcgagctcgc cgcaatccgt cagcagcagc aggccggccg tattgatgtc
      841 caggcgcccg accgcgaccc acttgccccc cggaatcggc ggcagccgct ggaataccgt
      901 gggccggccc tcgggatccg agcgcgtcac catctccccg tccggcttgt ggtacaggag
      961 cacgcgcggg cgcgcgccgg tggccttgag cgccaggacc ttgccgtcca gctcgatcac
     1021 atcgccgggg cgggcgcgct cgcccagctt cgtcacgacg ccgttgacgc gcaaccgccc
     1081 ggcggcgatc cagccctcga tctcccggcg cgagccgaat cccgccgccg ccaggagctt
     1141 ctgcagccgc tcagcctgcg gcgggttcgt tggcggcttc ggggacagcg ggcgccggcg
     1201 ggggctcaag ctgcagggtc tgcgcgatct cctccagggg cggcaattcc tgcagggagc
     1261 gcaagcccag ttcgtcgagg aaggtcttgg tcgtggcgta gagcgccggg cggcccggcg
     1321 tctcgcgatg cccgaccacg tcgatccagc cgcgcgcctc gagcgtctgc atgatctgcg
     1381 tggacaccgt cacgccgcgg atgtcctcga tgtcgccgcg cgtcaccggc tgccggtagg
     1441 cgatgatcgc cagcgtctcg agcaccgccc gcgagtagcg cggcggcttc tccggctgca
     1501 gcttctcggc gaacggcctg aactccggcc gggtctggaa gcgccatccg ctggcgaggc
     1561 tcaccagctc cacggcgcgc ccgctccact cctcgcgcag ctcggccagc aggttcttca
     1621 gggtctcggc gcccagctga tcgtcgaaca gtccgcgcag cgcggcgagc gacagcggct
     1681 cctgcgcgca caggagcgcc gcctcgagga tgcgcttggc ttcagtgggc tgcatgggcc
     1741 aattttacgt agatcggggc gaagctctcc gattgcgtga gctcgagcag cgactcgcgc
     1801 gccagctcca gcacggcgag cagcgtgacc accagcacgg cgacgccgcg cgcgggatcg
     1861 aacagctcgc cgaactcgag cacccgccgc tcctgcagcc ggcgcaggat gccgctcatg
     1921 tgctcgcgca ccgacagctc ttcgcgcgag acgcggtggt gcgcgctcat cctcgcccgg
     1981 tgcagcagcg tgcgccaggc ctcggcgagg tcctgggggt cgacgccagg cagctgctcg
     2041 gtcactacgc gctccaccca gaccgacacc gccagcacgt cgcgccccag ctgcggcagc
     2101 tggtcgagct gctgcgcggc cttcttgatg cgctcgtact ccagcagccg gcgcaccagc
     2161 tcggcgcgcg ggtc
//
LOCUS       DASPGE010000020         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_825819, whole genome shotgun sequence.
ACCESSION   DASPGE010000020 DASPGE010000000
VERSION     DASPGE010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825819"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VD791_00555"
     CDS             <1..904
                     /locus_tag="VD791_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV06494.1"
                     /translation="YGIIQLINFAHGEVVMIGAMVAFSVITALAGSGMPPLIIVLTGV
                     ACAVPACMLIGYGMERLAYRPLRRAPRLAPLITAIGVSIILQHLALLVWSRNVMAFPQ
                     IIQSRTFTIAGAAITNVQVAIVLLCVAIMSALAYLVYRTRLGTAMRATAQNPQVAGLM
                     GVDVNRVIAATFVIGAGLAAVAGVMVGTYYGIAQYTMGSILGLKAFAAAVLGGIGNIP
                     GAMLGGVLLGVVEALGAGYIGDLTDLCRYAWLAGLVEERCARDGHFILFGSNYQDVFA
                     FVVLILVLVFRPSGLLGERVGDRA"
     gene            909..1985
                     /locus_tag="VD791_00560"
     CDS             909..1985
                     /locus_tag="VD791_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV06495.1"
                     /translation="MRALFDTKSDPRLVWAGVAVVSLVLLVLPFLLAQIGTTWVRITN
                     YAILYVLLALGLNIVVGFAGLLDLGYIAFYAVGAYTYALLASPHFGLHLPFWTVLPIG
                     ALVAAFFGVLLGAPTLKLRGDYLAIVTLGFGEIVRIFLNNLSRPVNLTNGPQGITGID
                     SFKLGGLDFAASERVLGLTFSGPIKYYYLLLLVMIGILALNVRLQNSRIGRAWEAVRE
                     DETAARAMGINTTHMKLLAFAMGATFGGVAGGMFSAIQGFISPESFILVESILVVSMV
                     VLGGMGNIWGVVLGALLLSFVPEILRWTVEPLQRAAFGRMIVEPEVIRMLLFGLALVL
                     VMLFRPAGLLPSAVRKRELEEHVA"
BASE COUNT          249 a          692 c          673 g          393 t
ORIGIN      
        1 gtacggcatc atccagctca tcaacttcgc gcacggcgaa gtggtgatga tcggcgccat
       61 ggtggccttt tccgtgatca cggcgctcgc cggctccggc atgccgccgc tcatcatcgt
      121 gctgaccggc gtcgcctgcg cggttccggc ctgcatgctg atcggctacg gcatggaacg
      181 gctcgcctac cggccgctgc ggcgcgcgcc gcgcctcgcg ccgctcatca ccgccatcgg
      241 cgtctccatc atcctgcagc acctggcgct gctcgtctgg agccgcaacg tgatggcgtt
      301 tccgcagatc atccagagcc ggacgttcac catcgcgggc gcggccatca ccaacgtgca
      361 ggtggcgatc gtgctgctgt gcgtcgccat catgtcggcg ctcgcttacc tcgtgtaccg
      421 cacgcgcctg ggcacggcga tgcgcgccac cgcgcagaat ccgcaggtcg cgggcctgat
      481 gggcgtggac gtgaatcgcg tcatcgccgc gactttcgtc atcggcgcgg gcctggccgc
      541 ggtcgccggc gtgatggtcg gcacctacta cggcatcgcc cagtacacga tgggctcgat
      601 tctcggcctc aaggcgttcg ccgcggcggt gctgggcggt atcggcaaca tcccgggcgc
      661 gatgctgggc ggcgtgctgc tcggcgtggt cgaggcgctc ggcgcgggct acatcggcga
      721 cttgaccgac ctgtgccgct acgcctggct cgccggactg gtggaggagc gctgcgcgcg
      781 ggacgggcac ttcatcctct tcggctccaa ctaccaggat gtgttcgcct tcgtcgtgct
      841 gatcctggtg ctcgtcttcc ggccctccgg gctgctcggc gagcgcgtcg gcgaccgggc
      901 ctagccgcat gcgcgcgctg ttcgacacca agagcgaccc gcgcctggtc tgggcgggcg
      961 tggcggtcgt gtcgctcgtg ctgctcgtgc tgccgttcct gctggcgcag atcggcacca
     1021 cctgggtgcg catcaccaac tacgccattc tctacgtgct gctcgcgctc ggcctcaata
     1081 tcgtcgtcgg cttcgccggc ctcctggatc tgggctacat cgccttctac gccgtcggtg
     1141 cctatacgta cgcgctgctc gccagcccgc acttcggcct gcacctgccg ttctggaccg
     1201 tcctgccgat cggcgcgctg gtggccgcct tcttcggcgt gctgctgggc gcaccgacgc
     1261 tcaagctgcg cggcgactac ctggccatcg tcacgctcgg cttcggcgaa atcgtgcgta
     1321 tttttctgaa caacctgtcg cgcccggtga atctgaccaa cgggccgcag ggcatcacgg
     1381 gaatcgattc cttcaagctc ggcggcctgg acttcgcggc cagcgagcgt gtgctcgggc
     1441 tcacgttcag cggcccgatc aagtactact acctgttgct gctcgtcatg atcggcatcc
     1501 tcgcgctcaa cgtccgcctg cagaactcgc gcatcggccg cgcctgggaa gcggtgcgcg
     1561 aggacgaaac cgccgcgcgc gccatgggca tcaataccac gcacatgaag ctgctcgcct
     1621 tcgccatggg ggcgactttc ggcggcgtcg cgggcggcat gttctccgcc atccagggct
     1681 tcatctcgcc ggagagcttc atcctggtcg aatcgatcct ggtggtgtcc atggtggtgc
     1741 tgggcggcat gggcaacatc tggggcgtgg tgctgggggc gctgctgctg tccttcgtgc
     1801 cggagatcct gcgctggacg gtcgagccgc tgcagcgcgc ggccttcggc cgcatgatcg
     1861 tcgagcccga ggtgatccgc atgctgctgt tcggcctggc gctggtgctg gtgatgctgt
     1921 tccggcccgc agggctgctg ccttccgcgg tgaggaagcg cgagctggag gagcacgtgg
     1981 catgagcctg ctcaccgggg atcgcgt
//
LOCUS       DASPGE010000021         4843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1528330, whole genome shotgun sequence.
ACCESSION   DASPGE010000021 DASPGE010000000
VERSION     DASPGE010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528330"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="VD791_00565"
     CDS             complement(<1..1362)
                     /locus_tag="VD791_00565"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXV06496.1"
                     /translation="MPIAENLDLEAPATPGAPLLDPQAVAIAPQAQPLQQPDAAREPQ
                     AEPANAFAALTNFTVMMVDDDPIMLEVVQTFLEEAGYSSFVTTSDPTEAMALFVKQRP
                     DILLLDLMMPQVSGFDILGEVRSHEELRYTPVIMLTAESESSAKLKALELGATDFLLK
                     PVDPSELRLRLRNALAFKAYQDRLSDFDALTGLPNRRRFQAEVSAVLAKAAEQRSRAC
                     ALLHIDLDRFKQVNDTLGHRIGDKLLCGVAQMLARIADTSKPSQFPGSDQGARPSVAR
                     IGGNGFAVLLPNLHNLAKQDDATAVTRRILNTFAEPFHAEGHELFTTASIGIAVSPSD
                     GQDAEALLKNAEMAMYQAKKRGRGGYEFFSGDMNAQALERLTLENQLRRAVEREEFVL
                     YFQPKVEIAGRRIAGVEALIRWKHPEQGIIVPQKFIPIAEETGLIVEIGQWVLRTACS
                     QLQA"
     gene            complement(1381..3948)
                     /locus_tag="VD791_00570"
     CDS             complement(1381..3948)
                     /locus_tag="VD791_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV06497.1"
                     /translation="MRRPSARFFVSLGLASLLASVLLLAMYLDIMPDRHAAIRQGRGA
                     LAEAIAVNTSALLGDQDMARAKAALQFVVERNEDLLSAAVIKADGKAVATIGEHAEHW
                     KPLPGGLSTDAQIQVPILAGAQKWGRVELRFEPLRKPGWLGSFREPRVEMIGFVVLLS
                     AIAFYYYLGRVLRQLDPSRAIPGRVRAALDTMAEGLLVIDPKGYIMLANQAFAEVVGE
                     ASDKLIGRLATNFAWLGPDGVKLAAQACPWNATLRDAQALRHVLVRLADSSAKVRSFM
                     VNCSPVMGASGKAQGVLISFDDVTVLQEKEVELRIAKEQAEEANRSKSDFLANMSHEI
                     RTPMNAILGFTDVLRRGFYKSEGEMRRHLNTIHSSGKHLLELINDILDLAKVESGRME
                     VERLECAPHQIIQEVVEVLAVKAREKGIGLEFECSGPVPEKVLTDAGRVRQIVTNLTG
                     NAIKFTQSGLVKIIQGVRQDGGRAILTIDVVDTGVGIPENKLESIFDPFTQAESSTTR
                     RFGGTGLGLTISRRFARALGGDIVAHSVLGQGSVFSVTLDPGPLAGVRMLTPEQATVR
                     THEAGPLDSAAWQFPPAKVLVVDDGEENRELVRLVLEEAGLSVAQAENGRIGVDKALA
                     ESPEVILMDMQMPVMDGFTATQMLRKHGFKRPIVALTANAMKGFEQTILQAGCSHYLT
                     KPIDIDLLLQTLAGLLNGRRVAAAARPEVRREPAHAEPQAARAPAAHAPGVEQPRPEA
                     RAPAGPPIVSRLANHPKLRPVVRKFALQMSERMSAIEAAWQARDYPTLAGLAHWLKGS
                     GGTAGFDAFTAPARTLEQLAKAGDAAPIAAVIAELRALVDRVEAPVEDEDAPLARAG"
     gene            complement(3945..>4843)
                     /locus_tag="VD791_00575"
     CDS             complement(3945..>4843)
                     /locus_tag="VD791_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06498.1"
                     /translation="GGSIGSTAFNVSVGPPPIIVAPPPPPPPPPPPPPDPGTEPAPDP
                     GTEPAPSGEPTDTGASVPLPVGPGLDETLSDAQPTEPADANVIRTSANSSGRVPIAHL
                     LGDRSLVSVLSDRDGMLAPVPVEMGAAPELSVAGSLLSAPQFQRSDLAVLESVTSTLG
                     SSEFVGELDTMHDQVDGKIEVQDKLVASGVAVSGGLSVGYVIWLLRGGLLLSSLLSSL
                     PAWHAVDPMPVLARGGGDDDEEGADEDPLERLFGKAKDALLGGLRGRAVEPEPPPPVP
                     DDVDANAASRQQTRNDTAEANA"
BASE COUNT          669 a         1707 c         1685 g          782 t
ORIGIN      
        1 tgcctgcagc tgcgagcagg cggtgcgcag cacccattgc ccgatctcga cgatgagccc
       61 ggtctcctcg gcgatgggga tgaacttctg cggcacgatg atgccctgct ccggatgctt
      121 ccagcggatc aacgcctcga ccccggcgat gcgccggccg gcgatctcga ccttgggctg
      181 gaagtagagc acgaactcct cgcgctccac cgcgcggcgc agctggttct cgagcgtcag
      241 ccgctcgagc gcctgcgcgt tcatgtcgcc ggagaagaac tcgtagccgc cacgcccgcg
      301 cttcttggcc tggtacatgg ccatctcggc gttcttcagc aaggcctcgg catcctggcc
      361 gtcgctcggc gagactgcga tgccgatgct ggcggtggtg aacagctcat gaccctcggc
      421 atggaacggc tcggcgaagg tgttgaggat gcggcgggtg acggcggtgg cgtcgtcctg
      481 cttggcgaga ttgtgcaggt tgggcagcag caccgcgaaa ccgttgccgc cgatgcgcgc
      541 gaccgacggc cgggccccct ggtcgctgcc ggggaactgc gatggcttgc tggtgtcggc
      601 gatgcgcgcg agcatctgcg ccacgccaca cagcagcttg tcgccgatgc ggtggccgag
      661 cgtgtcattc acctgtttga agcggtccag gtcgatgtgc agcagcgcgc acgcgcgcga
      721 gcgctgctcg gcggccttcg ccagcaccgc ggacacctcc gcctggaacc tgcgccggtt
      781 cggcagcccg gtgagcgcgt cgaagtcgga caggcggtcc tggtaggcct tgaacgcgag
      841 tgcgttgcgc agccgcaggc gcagctcgct ggggtcgacc ggcttcagca ggaagtcggt
      901 tgcgcccagt tccagcgcct tgagcttggc cgagctctcg ctttcggcgg tgagcatgat
      961 caccggtgtg tagcgcagct cctcgtgcga acgcacctcg ccgaggatgt cgaagccgct
     1021 cacctgcggc atcatcaggt cgagcagcag gatgtcgggc cgctgtttga cgaacaacgc
     1081 catcgcctcg gtcgggtcgc tggtggtcac gaagctgctg tagccggcct cctccaggaa
     1141 ggtctgcacc acctcgagca tgatgggatc gtcgtccacc atcatgacgg tgaaattggt
     1201 gagcgccgcg aaggcgttgg ccggctccgc ctggggttcg cgcgcggcgt ccggctgctg
     1261 cagcggctgc gcctgcgggg cgatcgccac cgcctgcgga tccagcagcg gtgcgcccgg
     1321 cgtcgccggg gcttccaggt cgaggttctc agcgataggc atcggtcatg ctcctgagtt
     1381 ctagcctgct cgcgccagcg gcgcatcctc gtcttcgacc ggcgcctcca cgcggtccac
     1441 cagagcgcgc agctcggcga tcaccgccgc gatgggcgcc gcgtcgccgg ctttggcgag
     1501 ctgctccagc gtgcgcgccg gcgcggtgaa ggcgtcgaac ccggcggtgc cgcccgagcc
     1561 cttcagccag tgagcgagcc cggccagcgt cggatagtcg cgcgcctgcc aggccgcctc
     1621 gatcgcgctc atgcgctcgg acatctgcaa tgcgaacttg cgcaccaccg ggcgcagctt
     1681 gggatggttc gccaggcgcg atacgatcgg cgggccggcc ggcgcgcgcg cctccggccg
     1741 cggctgctcc acacctggcg cgtgggcagc cggcgcgcgg gcggcctgcg gctcggcgtg
     1801 cgccggctcg cgccgcactt cggggcgcgc cgccgccgcc acgcggcgtc cgttgagcag
     1861 gccggcgagc gtctgcagca gcaggtcgat gtcgatcggc ttggtcaggt agtggctgca
     1921 acccgcttgc aggatggtct gctcgaagcc tttcatggca ttggcggtga gcgcgacgat
     1981 cggccgcttg aagccgtgct tgcgcagcat ctgggtggcg gtgaagccgt ccatcaccgg
     2041 catctgcatg tccatgagga tgacttctgg gctctcggcg agcgccttgt ccacgccgat
     2101 gcgcccgttc tcggcctgtg cgacgctcaa gcccgcctcc tcgagcacca gacgcaccag
     2161 ctcgcggttc tcctcgccgt cgtccaccac cagcaccttc gccggcggga actgccacgc
     2221 ggcgctgtcc agcggcccgg cctcgtgcgt acgcactgtg gcctgctccg gggtcagcat
     2281 gcgcacgccc gccagcggac ccgggtcgag ggtgacgctg aacacgctgc cctgcccgag
     2341 cacgctgtgc gccacgatgt cgccgccgag cgcgcgcgcg aacctgcggc tgatggtcaa
     2401 tcccagaccg gtgccgccga agcgccgggt ggtcgagctc tcggcctgcg tgaagggatc
     2461 gaagatcgac tccagcttgt tctccgggat gcccaccccg gtgtcgacca cgtcgatggt
     2521 gaggatcgcg cggccgccgt cctggcgcac gccctggatg atcttgacca gtccgctctg
     2581 ggtgaacttg atggcattgc cggtcaggtt ggtcacgatc tgccgcacgc ggccggcatc
     2641 ggtgagcact ttctcgggca ccgggccgga gcactcgaac tccaggccga tgcccttttc
     2701 ccgcgccttc accgcgagca cctccaccac ctcctggatg atctggtgcg gtgcgcactc
     2761 gaggcgttcc acctccatgc gtccggactc gaccttggcc aggtcgagga tgtcgttgat
     2821 cagctcgagc agatgcttgc cgctggagtg gatcgtgttc aggtggcggc gcatctcgcc
     2881 ttcgctcttg tagaagccgc gccgcagcac gtcggtgaag ccgaggatgg cgttcatcgg
     2941 cgtgcggatc tcgtggctca tgttggccag gaagtcgctc ttggagcggt tggcctcctc
     3001 ggcctgctcc ttggcgatgc gcagctcgac ctccttctcc tgcagcacgg tgacgtcgtc
     3061 gaagctgatc agcaccccct gggccttgcc gctggcgccc atcaccggcg agcagttcac
     3121 catgaaggag cgcaccttgg cgctgctgtc ggccagccgc accaggacgt ggcgcagcgc
     3181 ctgcgcgtcg cgcagcgtcg cattccacgg gcaggcctgc gcggcgagct tcacgccgtc
     3241 cggccccagc caggcgaagt tggtcgccag ccgtccgatc agcttgtcgg aggcctcgcc
     3301 caccacctcg gcgaacgcct ggttcgccag catgatgtag cccttgggat cgatcaccag
     3361 caggccctcg gccatggtgt ccagcgccgc gcgcacccgg ccgggaatgg cgcgcgacgg
     3421 atcgagctgg cgcagcacgc gtccaaggta gtaatagaac gcgatcgcgc tcagcagcac
     3481 cacgaaaccg atcatctcca cgcgcggctc gcggaaagag ccgagccagc cgggtttgcg
     3541 cagcggttcg aagcgcagct ccacccggcc ccacttctgc gccccggcca ggatcggcac
     3601 ctggatctgc gcgtcggtgg acagcccgcc gggcagcggc ttccagtgct cggcgtgctc
     3661 gccgatggtc gcgacggcct tgccgtcggc cttgatcacg gcggccgaca gcaggtcctc
     3721 gttgcgctcg accacgaact gcagcgccgc tttggcgcgc gccatgtcct ggtcgccgag
     3781 cagggcggag gtgttgacgg cgatggcttc ggccagcgcg ccccgcccct gccggatggc
     3841 cgcgtgccgg tcgggcatga tgtccaggta catcgccagc agcagcacgc tggcgagcag
     3901 cgaagccagc cccagagaca cgaagaaccg cgcgctagga cgcctcatgc gttcgcctcc
     3961 gctgtgtcat tgcgcgtctg ttgccggctc gcggcgttcg cgtcgacgtc gtccggaacg
     4021 ggcggcggtg gctcgggctc gaccgcccgg ccgcgcagcc cgcccagcaa cgcatccttg
     4081 gccttgccga acagccgctc gagcggatcc tcgtcggcgc cctcttcgtc gtcgtcgccg
     4141 ccgccgcgcg ccagcaccgg catcggatcc accgcgtgcc aggccggcag cgaagagagc
     4201 agactcgaca gcagcagacc gccgcgcagc agccagatca cgtacccgac cgacaagccg
     4261 ccgctgaccg ccacgccgga ggcgaccagc ttgtcctgca cctcgatctt gccgtcgacc
     4321 tggtcgtgca tggtgtccag ctcgccgacg aactcggagc tgccgagcgt gctcgtcacc
     4381 gattccagca cagcgagatc ggagcgctgg aactgcggcg cgctgagcag cgagccggcc
     4441 accgacagct cgggtgctgc gcccatctcc accggcacag gcgccagcat gccgtcgcgg
     4501 tcggagagca cgctgaccaa gctgcggtcg cccagcaggt gagcgatggg tacgcgcccg
     4561 ctgctgttcg cgctggtgcg tatgacgttg gcgtcggccg gctcggtggg ctgcgcatcc
     4621 gacagcgtct cgtccagccc agggccgacg ggtagcggga cgctggcgcc ggtgtccgtc
     4681 ggctcaccgg acggcgcggg ctccgtgccc gggtccggag ccggctcggt gcccgggtcc
     4741 ggagggggag gcggcggggg cggcgggggc ggcgggggcg cgacgatgat cggcggcggc
     4801 cccaccgaga cgttgaacgc ggtcgagccg atgctgccgc cgc
//
LOCUS       DASPGE010000022         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_240821, whole genome shotgun sequence.
ACCESSION   DASPGE010000022 DASPGE010000000
VERSION     DASPGE010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240821"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VD791_00580"
     CDS             complement(<1..446)
                     /locus_tag="VD791_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HXV06499.1"
                     /translation="MSVGRTLRHLLHPAWTVRRAFPAHSLEAIRRAIAQSEAGHGGEI
                     RFAVEHALDLPDLLRNLSARERAIDMFSNLRVWDTERNNGVLIYLLLADRDVEIVADR
                     GIHAQVGAAGWEAICRRMEAAFAAGRFEAGVLDGIQAIGEHLRRHFP"
     gene            complement(463..1338)
                     /locus_tag="VD791_00585"
     CDS             complement(463..1338)
                     /locus_tag="VD791_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011881321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HXV06500.1"
                     /translation="MERALAGLALLLAFTPGIAQDVPVPPLRARVTDLTATLSAEQAA
                     QLERKLADFEARKGSQIAVLIVPTTRPETIEQYSIRVVEAWKLGRKGVDDGALLLVAK
                     DDRELRIEVGYGLEGALSDLMSNRIVEDIIVPRFRQGDFPGGIEAGVEAMIKVVDGEP
                     LPAPAPRGRTWVRGVGGNLETVLLVGFVLVFVIGGVLRAIFGRLPAAALIAGATGFIA
                     WIIVSSLIAAAVVGGIAFLLSLVAGTQRMGRGGWHPGGFGGGGWSGGGGGWSGGGGWS
                     GGGGGFGGGGASGRW"
     gene            complement(1352..1969)
                     /locus_tag="VD791_00590"
     CDS             complement(1352..1969)
                     /locus_tag="VD791_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXV06501.1"
                     /translation="MKNRLLSWVLAAVATLGLGGCGYNTIQAEDEQVKAAWSEVINQY
                     QRRADLIPNLVATVKGIAQQELDVLTQVTEARARAGSIQATPELINDPQAFARFQQAQ
                     GELTSALSRLLVTVEAYPQIKSDANFRDLQAQLEGTENRIAVARKRYIETVQQFNTTV
                     RQFPVNLTAMLFGYDAKPNFTVENEAAIAQPPAVDFGKPAPGGSQ"
     gene            complement(2129..>2438)
                     /locus_tag="VD791_00595"
     CDS             complement(2129..>2438)
                     /locus_tag="VD791_00595"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXV06502.1"
                     /translation="NRVPEPSEAAACEPFLLRQIALIQPRLIVALGKTAASNLLRTDA
                     SIASLRGRLHDFRGTPLLVTYHPAYLLRNLPDKSKAWEDLCLAREVMQRLKAPAPAPT
                     "
BASE COUNT          353 a          901 c          810 g          374 t
ORIGIN      
        1 gggaaatgcc gccgcaggtg ctcgccaatg gcctggatgc cgtcgagcac gcccgcctcg
       61 aaccggccgg cggcgaacgc cgcttccatg cgccggcaga tcgcctccca gccggccgcg
      121 ccgacctgcg cgtggatgcc gcggtcggcg acgatctcga cgtcgcggtc cgccagcagc
      181 agatagatca gcacgccgtt gttgcgctcg gtgtcccaca cgcgcaggtt cgagaacatg
      241 tcgatcgcgc gttcgcgtgc gctcaggttg cgcagtagat cgggcaggtc gagcgcgtgc
      301 tccacggcga agcggatctc gccgccgtga ccggcctcgc tctgcgcaat ggcgcggcga
      361 atcgcttcca ggctgtgcgc cgggaaggcg cgccgcaccg tccaggccgg atgcagcagg
      421 tgccgcaggg tgcgtccgac gctcacctca ccatctcccc cctcaccatc tccccgatgc
      481 cccgccgccc ccgaagccgc cgcctccacc gctccagcca ccgcctccgc tccagccacc
      541 gcctccgccg ctccaacctc cgccgccgaa gccgcccggg tgccagccgc cgcggcccat
      601 ccgctgcgtc ccggccacca ggctgagcag gaacgcgatg ccgcccacga ctgcggccgc
      661 gatcagagag gaaacgatga tccatgcgat gaaaccggta gcgccggcga tcagcgcggc
      721 ggcgggcagg cgtccgaaga tcgcgcgcag cacgccgccg atcacgaaca ccaggacgaa
      781 gcccaccaag agcaccgtct ccaggttgcc gcccacgccc cgcacccagg tgcgtccgcg
      841 cggcgccggc gcgggcagcg gctcgccgtc caccaccttg atcatcgcct ccacgccggc
      901 ctcgatcccg ccggggaagt cgccctgccg gaaacgcggc acgatgatgt cctcgacgat
      961 gcggttggac atcaggtcgg agagcgcccc ttcgaggccg tagccgacct cgatgcgcag
     1021 ctcgcggtcg tccttcgcca ccagcagcag cgcgccgtcg tccacgccct tgcgcccgag
     1081 cttccacgcc tccaccaccc ggatcgagta ctgctcgatg gtttcgggcc gggtcgtggg
     1141 cacgatcagc acggcgatct ggctgccctt gcgcgcctcg aagtcggcca gcttgcgctc
     1201 gagctgcgcc gcctgctcgg cggacagcgt ggcggtgagg tcggtgacgc gcgcgcgcag
     1261 cggcggcacc gggacgtcct gggcgatccc gggcgtgaac gcgaggagca gcgcgagccc
     1321 ggctaacgcg cgttccatca tgcccgcgcg gctactggct gccgccgggc gcaggcttgc
     1381 cgaaatcgac cgccggcggc tgggcgatgg ccgcctcgtt ctccaccgtg aagttgggct
     1441 tggcgtcgta gccgaacagc atggcggtga ggttgaccgg gaactggcgc accgtggtgt
     1501 tgaactgctg caccgtctcg atgtagcgct tgcgcgccac cgcgatgcgg ttctcggtgc
     1561 cctcgagctg cgcctgcaga tcgcggaagt tcgcgtcgga cttgatctgc ggataggcct
     1621 ccaccgtcac cagcagccgc gacagggccg aggtcagctc gccctgcgcc tgctggaagc
     1681 gggcgaaggc ctgcgggtcg ttgatcagct ccggcgtggc ctggatgctg cccgcgcgcg
     1741 cacgcgcctc ggtgacctgc gtcagcacgt ccagctcctg ctgcgcgatg cccttcacgg
     1801 tggccaccag gttgggaatc aagtcggccc ggcgctgata ctggttgatc acttcgctcc
     1861 acgccgcctt gacctgctcg tcctcggcct ggatcgtgtt gtagccgcag ccgcccaggc
     1921 cgagcgtcgc aaccgcggca agtacccaac tcagcagcct gttcttcatg tcggtggtct
     1981 cctgtcgagc tcctcgcaaa tggtgcccca ccccgtcgcg cttccagcgc cttcggcgcc
     2041 agcccggaat aggtagttta aatcgcgcct tcagggcgca ttcggcagtg tgcagcatcc
     2101 gcggccgtca tatcggggcc ggttgctgtc atgtcggggc tggcgccggg gccttcaagc
     2161 gctgcatcac ctcgcgcgcc aagcacaggt cctcccaggc cttggacttg tccggcagat
     2221 tgcgcagcag gtatgccgga tgataggtga ccagcagtgg ggtgccgcga aaatcgtgca
     2281 gccggccgcg caggctcgcg atcgaggcat ccgttcgcag caggttcgac gccgccgtct
     2341 tgccgagcgc cacgatcagg cggggctgga tgagagcgat ctgccgcagc aggaacggct
     2401 cgcacgcggc cgcttcgctc ggctccggca cgcggttg
//
LOCUS       DASPGE010000023         4380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1060480, whole genome shotgun sequence.
ACCESSION   DASPGE010000023 DASPGE010000000
VERSION     DASPGE010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060480"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1826
                     /locus_tag="VD791_00600"
     CDS             135..1826
                     /locus_tag="VD791_00600"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HXV06503.1"
                     /translation="MNRVKRNLLTGFAGAALATASAAAMAQAANEWLHYGGDQSNTRY
                     SSLSQINTGNVKNLRVLWMHSLGSLESQQSTPLVVGDTMYVTTSTGPKYVFALDAKSG
                     KVKWKHEPEMPNDYFATVCCGLDNRGVAYANGKVFFGRLDARLVALDANTGKQLWNVA
                     VADYKHGHAITSPPVVYKNLVVTGIAGGEYGIRGFVEAYDQNSGQSVWKTYTIPGAGE
                     PGNDTWKGDSWKTGAGSTWYVGSYDPKLNLVYWSTSNAGPWGGHTRGNDSSDIGQYTN
                     LWTSSQLAFDGDTGKIVWGYQYTPSDVWDYDGVNEAVLADVSIGGQKTPALLHADRNG
                     FFYVLNRQNGKLLAADPVVPVNWAKGIDMKTGMPIEDPAKRPRLGVWARNVCPNLIGG
                     KNWAPMSYNPQKGLTYVPLFNMCMDIASKEEEYTPGKFYLASEFNLDMAGSEGTNLSE
                     ILAWDPIGKKKVWSIKEDLPFLSGTMTTAGGLVFYGNQHGALKAVDAKTGNVLWQFNV
                     GTGILQSPITYTIGGKQYLAVVAGRTMGPPSFFGKIGQRVIDASPEGGVLVVFAL"
     gene            1954..2292
                     /locus_tag="VD791_00605"
     CDS             1954..2292
                     /locus_tag="VD791_00605"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV06504.1"
                     /translation="MAVAVLAMAPVAAAEAERPVNPYEGNAELVPEGKSLFNQYCSHC
                     HGPDAIQGERPRDLRRLRIRYGENAIGVFYTTINDGRMDKGMPPWKGVLREDVIWRIF
                     TFLSAVQTQP"
     gene            complement(2297..3136)
                     /locus_tag="VD791_00610"
     CDS             complement(2297..3136)
                     /locus_tag="VD791_00610"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXV06505.1"
                     /translation="MLTAQEIEHYRERGYVIPRYRLPDDLLRDVRAGLDRLLASYTDV
                     AQEDLANPHMLPPTDGPDINPFMRAARQPEILDMLAQLLGRDLVLWITRVLCKPAAKG
                     REVPWHQDGEYWPMRPLATCSVWIALDPATTGNGCMRFIPGSHKQQQLYRHHVTDRDN
                     LVLNLELDQDQFDEAQAVNVELEAGQMSLHDVRLIHGSLANTSGQRRAALIMRYMPAT
                     SHYDRSLVTQRRDNSPFNIYHQPLWLMRGVDRSGKNDFVHGHAQWRERYARGLVPAME
                     SEG"
     gene            complement(3173..>4380)
                     /locus_tag="VD791_00615"
     CDS             complement(3173..>4380)
                     /locus_tag="VD791_00615"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HXV06506.1"
                     /translation="QGVTTVVAGNCGVSLAPFPDGIRPPVTPPLDLLDESGGWFRFPT
                     FGAYVEELRARPPATNAVLLVGHTTLRVVVMDDLERPATPAEIGRMRELVREALAAGA
                     AGVSTGLYYEPAAAAPTEEVIEVCRPLREAGGLYCTHMRNEAERVLDSLEESFRIGRE
                     LGVPVVISHHKVAGTPNHGRSRETLALISRRMREQPIGLDCYPYCASSTILSADRAQF
                     ASRTLVTWSKPQPQCAGRDLAQIAKEWGVSQDEAVARLLPAGGIYFSMDEDDVQRILA
                     FPPTMIGSDGLPHDAFPHPRLWATFPRVLGHYSRELKLFTLEQAVHKMTGLTAQTYGL
                     DGRGVLKAGAHADIAVFEAAGVDEGATFERPIAAAKGIDTVIVNGTPVWREGKPTGAR
                     PGRVLRRTQ"
BASE COUNT          745 a         1469 c         1499 g          667 t
ORIGIN      
        1 cgggtcgacc gaccggaggg tgataagggg cctatcgcgg ggtggagcgg gcgacggcgg
       61 cctggggcaa aacgaggctg ccttggccgg ctctggcgca ccgcaggtcc tcaactttgg
      121 gggagacaac caagatgaat cgggttaaac gcaatctgct gaccggcttc gccggagcgg
      181 cgctggcgac agcgtcggcc gcggcgatgg ctcaggccgc caacgagtgg ctgcactacg
      241 gcggcgatca gtccaacacg cgctacagct cgctgagcca gatcaacacc ggcaacgtga
      301 agaacctgcg ggtgctgtgg atgcactcgc tgggcagcct ggagtcccag caatccacgc
      361 cgctggtggt gggcgacacc atgtacgtca ccacctccac cgggccgaag tacgtcttcg
      421 cgctcgacgc gaagtccggc aaggtcaagt ggaagcacga gccggagatg ccgaacgact
      481 acttcgccac ggtgtgctgc gggctggaca atcgcggcgt ggcctacgcc aacggcaagg
      541 tgttcttcgg ccggctggac gcacgcctgg tcgcgctcga cgcgaacacc ggcaagcagc
      601 tgtggaacgt ggccgtggcg gactataagc acggccacgc catcacctcg ccgccggtgg
      661 tctacaagaa cctggtggtg accggcatcg ccggcggcga gtacgggatc cgcggcttcg
      721 tcgaggccta cgaccagaac agcggccaat cggtgtggaa gacctacacc attcccgggg
      781 cgggcgagcc cggcaacgat acctggaagg gcgactcctg gaagaccggc gccggctcga
      841 cctggtacgt gggctcgtac gatccgaagc tgaatctggt gtactggtcc accagcaacg
      901 cggggccctg gggcggtcac actcgcggca acgactccag cgatatcggt caatacacca
      961 acctgtggac ctcctcccag ctcgccttcg acggggacac cggcaagatc gtgtggggct
     1021 accagtacac gccgagcgac gtgtgggatt acgacggcgt gaacgaggcg gtgctggcgg
     1081 acgtcagcat cggcgggcag aagacgcccg cgttgctgca cgccgaccgc aacggcttct
     1141 tctacgtgct caaccgccag aacggcaagc tgctcgccgc cgacccggtg gtgccggtca
     1201 actgggccaa gggcatcgac atgaagaccg gcatgccgat cgaggacccc gccaagcgcc
     1261 cgcggctggg cgtgtgggcg cgcaacgtct gcccgaacct gatcggcggc aagaactggg
     1321 cgccgatgtc gtacaacccg cagaaggggc ttacgtacgt gccgctgttc aacatgtgca
     1381 tggacatcgc cagcaaggaa gaggagtaca ccccgggcaa gttctacctg gcctccgagt
     1441 tcaacctcga catggcaggc agcgaaggca ccaacctgtc cgagatcctc gcctgggacc
     1501 cgatcggcaa gaagaaggta tggagcatca aggaagacct gccgttcctg agcggaacca
     1561 tgaccacggc cggcgggctg gtgttctacg gcaaccagca cggcgcgctc aaggcggtgg
     1621 acgccaagac cggtaacgtt ctgtggcagt tcaacgtggg cacaggcatc ctgcagagcc
     1681 cgatcaccta cacgatcggc ggcaaacagt acctggcggt ggtcgcgggc cgcaccatgg
     1741 gcccgccctc gttcttcggc aagatcggtc agcgcgtgat cgacgccagt cccgaaggcg
     1801 gcgtgctggt ggtgttcgcg ctgtaagccg gcacggtacg cgatgatggg aggcggcggg
     1861 gcgctcccac gccctgccgc ctttttttgt cgactcgagc gtacggagga tcgagtggca
     1921 gtgaagtccg ggagcgcaac cttgcgccgc gcaatggccg tggccgtcct cgccatggcg
     1981 ccggtcgcgg cggccgaggc cgaacgcccg gtgaatccct acgaaggcaa cgccgagctg
     2041 gtgcccgagg gcaagagcct gttcaaccag tactgctcgc actgccacgg ccccgacgcg
     2101 atccagggcg agcgcccgcg cgacctgcgc cggctgcgca tccgctatgg cgaaaacgcc
     2161 atcggcgtgt tctacaccac catcaacgac gggcgcatgg acaagggcat gccgccctgg
     2221 aaaggcgtgc tcagggaaga cgtcatctgg cgcatcttca ccttcctgtc cgcggtgcag
     2281 acgcagccat gagttcttac ccctcgctct ccatcgccgg caccagacct cgagcgtagc
     2341 gctcgcgcca ctgcgcgtgg ccgtgcacga agtcgttctt gccgctgcgg tccacgccgc
     2401 gcatcagcca cagcggctgg tgatagatgt tgaaggggct gttgtcgcgg cgctgggtga
     2461 ccagcgaacg atcatagtgc gaggtggcgg gcatgtagcg catgatcagc gccgcccggc
     2521 gctggcccga ggtgttggcc agcgagccgt gaatcagccg cacgtcgtgc agcgacatct
     2581 gccccgcttc cagctccacg ttcaccgcct gcgcctcgtc gaactggtcc tggtccagct
     2641 cgaggttgag caccaggttg tcgcggtcgg tcacgtgatg gcgatagagc tgctgctgct
     2701 tgtgcgaccc ggggatgaag cgcatgcagc cgttgccggt ggtcgccggg tcgagcgcga
     2761 tccacaccga gcaggtggcg agcggccgca tcggccagta ctcgccgtcc tggtgccagg
     2821 gcacttcgcg gcccttcgcc gccggcttgc acagcacgcg cgtgatccac agcaccaggt
     2881 cgcgaccgag cagctgcgcc agcatgtcca ggatctccgg ctggcgcgcc gcgcgcatga
     2941 agggattgat gtccggcccg tcggtgggcg ggagcatgtg cgggttggcg aggtcttcct
     3001 gcgccacgtc ggtgtagctc gccagcagcc ggtccaatcc cgcgcggaca tccctcagca
     3061 ggtcgtccgg gagccggtaa cgcgggatca cgtagccgcg ctcgcgatag tgctcgatct
     3121 cttgtgcggt cagcatggga attcgattcg actgttcagg ctacattatc gcttactgcg
     3181 tgcggcgcag caccctgccc ggccgcgcgc cggtgggctt gccttcgcgc cacaccgggg
     3241 tgccgttcac gatcaccgtg tcgatgcctt tcgccgccgc gatgggacgc tcgaaggtcg
     3301 cgccctcgtc cacccccgcg gcttcgaaca ccgcgatatc ggcgtgggcg cccgccttca
     3361 gcacgccgcg cccgtcgagg ccgtaggtct gcgcggtgag cccggtcatc ttgtgcaccg
     3421 cctgctccag cgtgaacagc ttcagctccc ggctgtaatg ccccagcacg cgcgggaacg
     3481 tggcccacag gcgcggatgc ggaaaggcgt cgtgcggcag gccgtcggag ccgatcatgg
     3541 tcggcggaaa ggccaggatg cgctgcacgt cgtcctcgtc catggaaaag tagatccccc
     3601 ccgcgggcag cagccgcgcc accgcttcgt cctgcgacac gccccactcc ttcgcgatct
     3661 gcgcgaggtc cctccccgcg cactgcggtt gcggcttcga ccacgtcacc agcgtcctgg
     3721 acgcgaattg cgcgcggtcg gcggaaagga tggtcgagga cgcgcaatag ggatagcagt
     3781 ccagcccgat gggctgctcg cgcatgcgcc tggagatcag cgccagcgtc tcgcgggagc
     3841 gcccgtggtt aggcgtgccg gcgaccttgt gatgcgagat caccaccggc acgcccagct
     3901 cgcggccgat gcggaagctc tcctccagcg agtccagcac ccgctcggcc tcgttgcgca
     3961 tgtgcgtgca gtacagcccg ccggcctcgc gcagcggccg gcacacctcg atcacctcct
     4021 cggtgggcgc ggcggcggcg ggttcgtagt acaggccggt cgagaccccg gcggcgccgg
     4081 cggcgagcgc ctcgcgcacc agctcgcgca tgcggccgat ctcggccggg gtagcgggac
     4141 gctcgaggtc gtccatgacg accacgcgca gcgtggtgtg gccgaccagc agcacggcgt
     4201 tggtcgcggg cggacgagcg cgcagctctt ccacgtaggc gccgaaggtc ggaaagcgga
     4261 accagccgcc cgattcgtcc agcaagtcca gcggcggggt gacgggcggc cggatgccgt
     4321 cgggaaacgg cgccagggaa acgccgcaat tgcccgcgac caccgtggtg acgccctggc
//
LOCUS       DASPGE010000024         4502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_357652, whole genome shotgun sequence.
ACCESSION   DASPGE010000024 DASPGE010000000
VERSION     DASPGE010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357652"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VD791_00620"
     CDS             <1..1127
                     /locus_tag="VD791_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HXV06507.1"
                     /translation="AAFGSWLWYVLFRQLDRALFGTLQHVDAGKSVPLKATPHSLRGY
                     LKIVTIHGVPVFIHWSFPVGGLLISFWVGFDLEAAAYYCLGYLILVGAHELGHFAAAR
                     WSRLNVRAVEISGIGGLCRMEMPRTVGQAFLVYSGGFLAQLLLLILALSYVAAFGSPT
                     SRLAQCLAATFIVVNILVLVANIIPIKPREGVATDGYVLFKLLRGDSREIVAPVDSSP
                     VLPPDTRLLSIEGFVPDGFVSGIEILNDRTTPMEFVVGVLMKDLEHNREQAVEIMLTV
                     HRRGGLLIPVPTFEEATLIAERITLEAREHNHALICRAVNADHPKSEAQSGRRGFLTP
                     GSHNTPHAGPHGVFHEATYAESHVAMNDTQRRPLQRIGDK"
     gene            complement(1171..2673)
                     /locus_tag="VD791_00625"
     CDS             complement(1171..2673)
                     /locus_tag="VD791_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXV06508.1"
                     /translation="MTLHRVLERWAAIQPHKPAVHFQGADITYAALAHDTVRAAEGLA
                     QHLGVRPGDRVAYLGYNRPQMLVLLFAVARIGAMLLPLNVRLAPAEHRTILRHAEASA
                     VIADQAMLAAAAQLREALAGMRGVAVGATPAGWLGWEDLIAGGAPPLAQLGQARDPVL
                     LVYTSGTAGAPKGAVHTQEALIWNAVNAAHCHDLTSVDHVLTVLPMFHVGGLCIQTLP
                     ALHAGASVTLHERFDAGRWIADVRVRRPTLSLLVPATIKAILEHPDWPGADLSSLRAV
                     FTGSSIVPDALFAPFHARGVPLAQVYGATETGPVSICLRAADALRKSGSAGTVAIHCD
                     VRLMGGDGAEVARGQVGEIWVRGPSLMLGYWKDPHNPAFEQGWFRSGDLARQDEEGFY
                     WVAGRSQDMIVSGGENIYPAEIENVLVQCADIVEAAVIGTPDPQWGEVPVAAVVKRPG
                     SALDEAAVLRLFEDRLARFKRPRRVVFLASLPKTALGKVQKAVLRSALQR"
     gene            complement(2683..3924)
                     /locus_tag="VD791_00630"
     CDS             complement(2683..3924)
                     /locus_tag="VD791_00630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXV06509.1"
                     /translation="MPYRPPPIVGEGFPLTARQAQLLELASRLGRERFAPRAARYDRD
                     AAFPFENYADLREAGLLALCVPERYGGGGADLLTYCMVAAEIGRHCGATALTYNMHVC
                     STLWTGALADALTMTRQQRAEHERCRQLHFARVVEQGKLYAQPFSEGSAAAAGRAPFG
                     TLAAKVEGGWRVNGKKIFASLAGAADYYGVLCTEDKPDRSLRDTLYLAISAQAPGFSI
                     VGDWDPLGMRGTVSRTLLMQDVFVPDSAQLMPPGVYFQAASRWPHMFATLSPTYMGIA
                     QAAYDFTVQYLRGEAPGTPPVKRRMYPTKQVAVAQMHIQLEQTRALFLRTLSEAKVDP
                     GKDERLRVYAAQYTIMENANDLCRLAIRTCGGQSMLRSLPLERMYRDSRCGSLMLPWT
                     AELCLDRIGREALYEAGETDD"
     gene            complement(3933..>4502)
                     /locus_tag="VD791_00635"
     CDS             complement(3933..>4502)
                     /locus_tag="VD791_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXV06510.1"
                     /translation="GQLLDAVASERVVLVGHSMGGMVALEAMALFPERIAGLVLSATS
                     PAFGKPDGDWQRAFLRQRLEPLDQGKSMAELAPALVRGMVAPQADPAAVQRAVEIMSA
                     VPLATYRAALHALVTFDRRALLPSIAVPTLALAGEADSVAPPAVLEKMAGKIPGARYH
                     CIARAGHLLSLEQPAAFTEAVLRFVRTAL"
BASE COUNT          733 a         1514 c         1487 g          768 t
ORIGIN      
        1 tcgctgcgtt tggaagctgg ttatggtacg tcctattccg ccaactggat cgggctcttt
       61 tcggcaccct gcaacacgtt gatgcaggga aatccgtgcc tctgaaggcg acgcctcata
      121 gcctccgagg atatctgaag atcgtcacca tacacggtgt tcccgtcttc attcattggt
      181 ccttcccagt tggcgggctt ctaatttctt tctgggttgg ctttgatctg gaggcggcag
      241 cgtactactg cctgggctac ttgatcctgg tcggagctca tgagcttggc cactttgctg
      301 ctgcgcgctg gtcgcgattg aacgtgcggg ctgtagagat ctctggaatt ggcggcctat
      361 gccggatgga gatgcctcga acagtgggcc aagccttcct ggtgtattca ggaggctttc
      421 tggcgcaact gctgctgctg atacttgcac tgtcgtacgt tgccgctttc ggttctccga
      481 cgtctcgcct tgcgcaatgc ttggctgcca cgttcatcgt tgtgaacatt ctcgtcctgg
      541 ttgccaatat catccccatc aaaccacgtg agggcgtcgc cacggatggt tacgttctct
      601 tcaagctgct gagaggggat tcccgcgaaa tcgttgcacc ggtggatagc tcgcctgtat
      661 tgccgccaga tacacgtctt ctttcaatcg aaggctttgt gccggatggc tttgtcagtg
      721 ggatcgagat cttgaacgat cgaacaactc cgatggaatt cgtggtcggt gtactcatga
      781 aggatctaga gcataatcgg gagcaggcgg ttgaaataat gctcaccgtt catcggcgag
      841 gaggcctgct gataccagtg ccgacctttg aggaggctac actcatagcg gagcgcatta
      901 cgcttgaggc gcgcgaacac aatcacgctc ttatctgcag agcggtgaac gctgatcatc
      961 cgaagtccga ggcgcaatcg ggtcgccggg ggtttctaac tcccggctcc cacaacaccc
     1021 cgcatgcggg tccgcacgga gtgtttcacg aagcgacgta cgcggaatct catgtcgcca
     1081 tgaacgacac acagcgtcga ccactgcagc ggattggcga taagtgatcg aggcgctctc
     1141 aggcaaggcc cacacaccga atacggctga tcatcgctgc agtgcgctgc gcaacaccgc
     1201 cttctgcact ttgccgagcg cggtcttcgg caggctcgcc aggaacacca cgcggcgcgg
     1261 ccgcttgaag cgtgcgagcc ggtcttcgaa caggcgcagc accgcggctt cgtccagcgc
     1321 gctgcccggt ctcttgacca cggcggccac ggggacttcg ccccactgcg ggtcgggcgt
     1381 gccgatcacg gccgcttcga cgatatctgc gcattgcacc agcacgttct cgatctcggc
     1441 cggatagatg ttctcgccgc cggagacgat catgtcctgc gagcgccctg ccacccaata
     1501 gaacccctct tcgtcctggc gcgccaggtc gccgctgcgg aaccagcctt gctcgaacgc
     1561 cgggttgtgc gggtccttcc agtagcccaa catgagactg ggcccgcgca cccagatctc
     1621 gccgacctgt ccgcgcgcca cctcggcgcc gtcgccaccc atcaggcgaa cgtcgcagtg
     1681 gatcgcgacg gtgccggcgg agccgctctt gcgcagggcg tccgcagcgc gcagacagat
     1741 cgagaccggc ccggtttcgg tcgccccata cacctgcgcg agcggtacac cgcgcgcgtg
     1801 gaacggcgcg aacagcgcat cggggacgat cgaggagccg gtgaacaccg cgcgcagcga
     1861 tgacaggtcg gcgccgggcc agtcgggatg ctcgaggatc gccttgatgg tggccggcac
     1921 cagcaacgac agtgtcggcc gccggacgcg cacgtcggcg atccagcgcc ccgcatcgaa
     1981 gcgctcatgc agcgtcacgc tcgctcccgc gtgcagcgca ggcagcgttt ggatgcacaa
     2041 cccgccgacg tggaacatgg gcagaaccgt cagcacgtga tcgacgctgg tcagatcgtg
     2101 gcagtgcgcg gcgttgaccg cgttccagat cagcgcctcc tgcgtgtgca ccgcgccctt
     2161 cggtgccccg gcggtgcccg aggtgtagac cagcagcacc ggatcgcgcg cctgaccgag
     2221 ctgcgcgagc ggcggcgcgc cgccggcgat caggtcttcc catccgagcc agccggcggg
     2281 cgtggcgccg accgctacgc cgcgcatccc ggccagcgct tcgcgcagtt gcgcggcggc
     2341 agcgagcatg gcctggtccg cgatcaccgc gctcgcctcg gcatgccgca agatggtccg
     2401 gtgctcggcc ggcgcgaggc ggacgttgag cggcaacagc atcgccccga tgcgcgccac
     2461 cgcaaacagc aacaccagca tctgcggccg gttgtagccg aggtaggcga cgcggtcgcc
     2521 gggacgcaca cccaggtgct gcgccagccc ctcggcggcg cgcacggtat cgtgcgccag
     2581 cgctgcgtag gtgatgtccg caccctggaa gtgcaccgcc ggcttgtgcg gctggatcgc
     2641 cgcccagcgc tcgaggacgc gatgcagggt catgcgcttc gctcagtcgt ccgtctcgcc
     2701 cgcctcgtaa agcgcttcgc gaccgatgcg atcgaggcac agctcggcgg tccagggcag
     2761 catcagcgag ccgcagcggc tgtcgcggta catgcgctcg agcggcaggg agcgcagcat
     2821 cgattgcccg ccgcaggtgc ggatcgccag gcggcacagg tcgttggcgt tctccatgat
     2881 ggtgtactgc gccgcataca cccgcaggcg ctcatccttg cccggatcca ccttcgcctc
     2941 ggacagcgtg cgcaggaaca gcgcgcgggt ctgctcgagc tgaatgtgca tctgcgccac
     3001 cgccacctgc ttggtcggat acatgcgccg cttcaccggc ggcgtgccgg gcgcctcgcc
     3061 gcgcagatac tgcacggtga agtcgtaggc ggcctgggcg atgcccatgt aggtgggtga
     3121 gagcgtggcg aacatgtgcg gccatcgcga agcggcctgg aagtagacgc ccggcggcat
     3181 gagctgggcg ctgtcgggga cgaacacgtc ctgcatcagc agcgtgcgcg acaccgtgcc
     3241 gcgcatgcct aggggatccc agtcgccgac gatggagaag ccgggcgctt gcgcggagat
     3301 cgccagatac agcgtgtcgc gcaggctgcg gtccggcttg tcttcggtgc acagcacgcc
     3361 gtagtagtcg gccgccccgg cgagcgatgc gaagatcttc ttgccgttca ccctccaacc
     3421 gccctccacc ttcgccgcca gcgtgccgaa cggcgccctg ccggcagcgg ctgcgctgcc
     3481 ctcggagaac ggctgcgcgt agagcttgcc ctgctccacc acgcgcgcga agtgcagctg
     3541 gcggcaacgc tcgtgctcgg cgcgctgctg tcgcgtcatg gtcaacgcgt cggccagcgc
     3601 accggtccac agggtagagc acacgtgcat gttgtaggtg agcgcggtgg cgccgcagtg
     3661 ccggccgatc tcggcggcca ccatgcaata ggtcagcagg tccgcgccgc cgcccccgta
     3721 acgctcggga acgcacaagg cgagcagccc cgcctcgcgc agatcggcgt agttctcgaa
     3781 cgggaacgcg gcgtcgcgat cgtagcgggc cgcgcgtggc gcaaagcgtt cgcgcccgag
     3841 ccgggatgcc agctcgagca gttgcgcctg cctggcggtc aacgggaagc cttcaccgac
     3901 gatgggcggc ggacggtacg gcatggacat tctcacagtg ccgtgcgcac gaatcgcagc
     3961 acggcctcgg tgaacgccgc cggctgttcc aggctcagca gatggccggc ccgcgctatg
     4021 cagtggtagc gtgcaccggg gatctttccg gccatcttct ccagcaccgc gggcggcgcc
     4081 acgctgtcgg cctcgccggc gagcgccagc gtgggcacgg cgatcgacgg cagcagcgcg
     4141 cggcgatcga aggtcaccaa tgcgtgcagc gcagcgcgat aggtggccag cggcacggcg
     4201 ctcatgattt ccacggcgcg ctgcacggcg gcaggatccg cttgcggcgc gaccatgccg
     4261 cgcaccagcg caggcgcgag ctcggccatg ctcttgcctt gatccagcgg ctcgagccgc
     4321 tggcgcagga acgcccgctg ccagtcgccg tcgggcttgc cgaaagcggg gctggtggcg
     4381 ctcagcacca ggccggcgat gcgctcgggg aacagcgcca tcgcttccag cgccaccatg
     4441 ccgcccatgc tgtgcccgac cagtaccacg cgctccgagg cgaccgcgtc caggagttgc
     4501 cc
//
LOCUS       DASPGE010000025         2730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_592515, whole genome shotgun sequence.
ACCESSION   DASPGE010000025 DASPGE010000000
VERSION     DASPGE010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592515"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VD791_00640"
     CDS             complement(<1..795)
                     /locus_tag="VD791_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXV06511.1"
                     /translation="MIEAPHQRASLGDLRLESGETIRDFELSYVTHGKLNAARDNAIL
                     VTVSLTGNHHRLDFLIGSGKALDPERHFIVCVDPIGNGLTTSPSNSSAQPGMKFPRFG
                     IRDMVRSQQRLLVDEMDLAGVHAVVGASMGGMQALQWAVSFPEFPRAVVAMTPMARTA
                     AWAVAVVETARRALTADPAWDGEQFTAYPERGWRAWTGVMSVLANRTPRALADMFLQP
                     LGALEWLDKLTADNKANGFDATDWLYQSWAYQEHDVGGTPGFHGDLE"
     gene            complement(822..1928)
                     /locus_tag="VD791_00645"
     CDS             complement(822..1928)
                     /locus_tag="VD791_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HXV06512.1"
                     /translation="MKFKAAVLNAVGGPLEIEELELSDLEPTDVLVRIHASGLCHTDL
                     EVIEGALAYPLPIVLGHEGAGVVEAVGSEVTLVARGDHVVCSWNPHCGHCFYCEQDLP
                     ILCEPFTRHQPKGLLLDGRSRLSRRGSQVHHFSVVSSHAQYCVVPESGAIAVPKELPL
                     DRACLIGCGVMTGVGAATRKAPVQPGAAVLVIGCGAVGLNVIQGARLAGAGLIVAADV
                     SDEKLDRAKGFGADTRLDAGAPEVLQLLRGMTQGRGPDYVFEAAGNDNAFRLALEAVR
                     PGGQVVFLGKVNVNREVAFRWGSLMGEKRIVRSSYGAGRPRRDFPWLAREYLAGRLKL
                     DELVTRRIALEDINSGFDAIRRGETVREVVVFPQ"
     gene            complement(2053..>2730)
                     /locus_tag="VD791_00650"
     CDS             complement(2053..>2730)
                     /locus_tag="VD791_00650"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXV06513.1"
                     /translation="RIPFRRKIAQLKGLALPILVIATVLGTIYAGVASVTEAAGMGVV
                     GTMIASAVRGELKWTHMRESLQQTMRTCGMLLWLSFGATAMIGVYNLAGGPAFVKQVM
                     TGMDLAPVGIIVVMMAILMVLGCIMDWVGICLLTMPIFVPIVRELGYSPIWFGVLFCL
                     NMQVSYLSPPFGPAAFYLKGVAPPDISLNQIFAALWPFILLQLTVLMVVLFFPQIALW
                     LPGLGTQ"
BASE COUNT          448 a          976 c          900 g          406 t
ORIGIN      
        1 ttccagatcg ccgtggaaac cgggcgtacc gcccacgtcg tgctcctggt aagcccagct
       61 ctggtacagc cagtcggtcg cgtcgaagcc gttggccttg ttgtccgcag tgagcttgtc
      121 cagccactcc agcgcgccga gcggttgcag aaacatgtcc gccagcgcgc gcggcgtgcg
      181 gttggccagc acgctcatca cgccggtcca cgcgcgccag ccgcgctccg ggtatgcggt
      241 gaactgctcg ccgtcccagg ccgggtcggc ggtgagcgcg cgccgcgccg tctccaccac
      301 cgccaccgcc caggcggcgg tgcgcgccat cggcgtcatg gccaccaccg cgcgcgggaa
      361 ctcggggaag ctcaccgccc attgcagcgc ctgcatgcct cccatcgacg cgccgaccac
      421 ggcgtgcacg ccggcgaggt ccatttcgtc caccagcagg cgctgctgcg agcgcaccat
      481 gtcgcggatg ccgaagcgcg gaaacttcat gcccggctga gcgctgctgt tgctcggcga
      541 ggtggtgagg ccgttgccga tcgggtccac gcacacgatg aaatggcgct ccggatcgag
      601 cgccttgccg gacccgatca ggaagtccag gcgatggtgg ttgccggtca gggacaccgt
      661 caccaggatg gcgttgtcgc gcgccgcgtt gagctttccg tgcgtgacgt aggacagctc
      721 gaagtcgcgg atcgtctcgc cgctctccag ccgcaggtcg cccagggacg cccgctggtg
      781 cggtgcctcg atcatgcctg cgcgcatcgg cgggtggcgg gtcattgcgg gaacacgacg
      841 acctcgcgca ccgtctcgcc gcggcggatg gcgtcgaagc cgctgttgat atcctccaat
      901 gcgatgcggc gggtgaccag ctcgtcgagc ttcagcctgc cggcgaggta ctcccgcgcc
      961 agccagggga agtcgcggcg cgggcgcccc gccccgtagc tggagcgcac gatgcgcttc
     1021 tcgcccatca acgagcccca gcggaaggcg acttcgcggt tgacgttgac cttgccgaga
     1081 aacaccacct ggccgccggg gcgcaccgcc tccagagcca ggcggaaggc gttgtcgttc
     1141 cccgccgcct cgaacacgta gtccggcccg cgcccctgcg tcatgccgcg cagcagctgc
     1201 agcacctcgg gcgcgccggc gtccaagcgc gtgtcggcgc cgaagccctt ggcgcggtcg
     1261 agcttctcgt cgctgacgtc ggcggccacg atcagccccg cgccggcgag ccgcgcaccc
     1321 tggatgacgt tcaatcccac cgcgccgcaa ccgatcacca ggacagcggc gcccggctgc
     1381 accggcgcct tgcgcgtggc cgcacccacg ccggtcatca cgccgcagcc gatcaggcag
     1441 gcgcggtcga gcggaagctc cttgggcacg gcgatcgcgc cggattccgg caccacgcaa
     1501 tactgagcgt gcgacgacac cacggagaag tgatgcacct ggctgcctct gcgcgacagg
     1561 cgcgagcggc cgtcgagcag cagccccttg ggctggtgcc gcgtgaacgg ttcgcacagg
     1621 atcggcaggt cctgctcgca gtagaagcaa tggccgcaat gcgggttcca ggagcacacc
     1681 acgtggtcgc cgcgcgcgac cagggtgacc tcgctgccga cggcctccac cacccccgcg
     1741 ccctcgtggc cgagcacgat cggcagcggg taagcgagcg cgccctcgat cacttccagg
     1801 tcggtgtggc acaggccgct ggcgtggatg cgcaccagca cgtcggtcgg ctcgagatcg
     1861 ctcagctcca gctcctcgat ctcgagcggc ccgcccactg cgttcagcac tgcggccttg
     1921 aatttcatgc cactacccct caaccgcaaa ggcgccgaga actgccgcaa agtactcggg
     1981 atggtattca cgcaggcggt gttctcgcgg gtttcctttg cgggtctcct ttgcgccttt
     2041 gcgcctctgc ggtcattgcg tacccaagcc cggcaaccat agggcgatct gcgggaagaa
     2101 caataccacc atcagcaccg tcagctgcaa caggatgaac ggccacaggg cggcgaagat
     2161 ctggttgagc gagatgtccg gcggcgccac ccccttgaga tagaaggcgg ccggcccgaa
     2221 gggcggcgac aggtagctca cctgcatgtt caggcagaac agcacgccga accagatcgg
     2281 gctgtagccc agctcgcgca cgatcggcac gaagatcggc atggtgagca ggcagatgcc
     2341 gacccagtcc atgatgcagc cgagcaccat caggatcgcc atcatcacca cgatgatgcc
     2401 caccggcgcc aggtccatgc cggtcatcac ctgcttgacg aacgccggcc cgccggcgag
     2461 gttgtacacc ccgatcatcg cggtggcgcc gaaggacagc cacagcagca tgccgcaggt
     2521 gcgcatggtc tgctgcagcg attcgcgcat gtgggtccac ttcagctcgc cgcgcaccgc
     2581 cgaggcgatc atggtgccga ccacccccat gcccgcggcc tcggtgaccg aagccacgcc
     2641 ggcgtagatc gtgcccagca ccgtggcgat gaccagtatc ggcaaggcca ggcccttgag
     2701 ctgggcgatt ttgcggcgaa acgggatgcg
//
LOCUS       DASPGE010000026        17799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_241711, whole genome shotgun sequence.
ACCESSION   DASPGE010000026 DASPGE010000000
VERSION     DASPGE010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17799
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241711"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VD791_00655"
     CDS             <1..439
                     /locus_tag="VD791_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06514.1"
                     /translation="RSSYNGLLLFLFIVAAAWLAATVIRRFASGALRRAPAWDCGFPD
                     ASPLTQYTSASFAQPIRRVFGSVVFHAREEVQIPAPGQTLPARFHVSMRDLVWDAVYA
                     PVESVVNWTSDRLNKVQFWTIRAYLTLVFGAVVLLLMVLAVWH"
     gene            430..1386
                     /locus_tag="VD791_00660"
     CDS             430..1386
                     /locus_tag="VD791_00660"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HXV06515.1"
                     /translation="MALILDALVQLAQLLLVLSLAPLLTGYVRKVKAHLLRRRGPPLV
                     QPYRDLLKLIRKEAVLAENASWVFRVAPYLIFATTLIAAALVPTFATGLVFSSAADLI
                     AIVALLGSARFALALAGLDIGTSFGGIGSSREMMIASLAEPAMLMIVFTLSLLAGSTQ
                     LSSVAAFMQGPEVGLRVSLAMSLVALIMVALAENARIPVDNPATHLELTMIHEAMILE
                     YSGRHLAVIEAAASLKLLLYLSLIACMFAPWGVATTGQGFAAYAIGLGTFLLKLAAGG
                     FLLAVFETVTAKMRVFRVSDFLGAALMLGLLAALLLFVSQSA"
     gene            1386..2048
                     /locus_tag="VD791_00665"
     CDS             1386..2048
                     /locus_tag="VD791_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HXV06516.1"
                     /translation="MDRLALDVAHLFAGGLVLVSFMLLYQDRMFGLLNIFALHALVVS
                     LSVAWQAYIQAAPHLYITAGIALVVKAIIIPVALHRIIVRLGIHRTIETVVGIGQTML
                     AGIALVALSILVMLPITAEAGTLAREDLAFSLSVILLGLLMMISRRNAVSQIIGFMSI
                     ENGLILAATAAKGMPLVVEISVAFSILVALIVIGIFLFRIRERFDTVDVHALERFRGE
                     QP"
     gene            2045..3493
                     /locus_tag="VD791_00670"
     CDS             2045..3493
                     /locus_tag="VD791_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HXV06517.1"
                     /translation="MIAPGPLLVQAILVVPAGSAALLALFPDDRATGRLNAVGTFLTF
                     LAAAGLFFVRPEPGPFLIVDDVNVVFIVLNTFVGFTTSVFSASYIAHELDVGRLTPTY
                     LRFYHAMYQTLMFAMNLALLANNVGLMWVAVELATLTTILMVGIYRTPEALEAAWKYF
                     ILGSVGIALALFGTILVYLAARSVVGEGLDAMAWNVLVGQAAAFDPTLLNLAFVFLLL
                     GYGTKVGLAPLHAWLPDAHAEGPTPISAVLSGLLLNVALFAVLRFKMVLSANPEALAP
                     GPLMVTLGLISVVFAAFMLYRRHDIKRMFAYSSIEHMGIITFAFGMGGPLANFAGLLH
                     MTFHSLTKSAIFFSVGHIAQVKGTQRMADIRGLTVSHPVLGWGLVLGVMTIVGLPPMG
                     IFMSEFLIVTSTFARQPVLAVALAAGLLVALGALLLRLNGLAFGDPHGSTAPAQASFV
                     PMFAHLALVLAAGIYLPAPLVAWFRHVAGLLG"
     gene            3585..5033
                     /locus_tag="VD791_00675"
     CDS             3585..5033
                     /locus_tag="VD791_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni,Fe-hydrogenase III large subunit"
                     /protein_id="HXV06518.1"
                     /translation="MIARESWQRAAGLLQAGRCTLLGLWGERAFVHLALVDERRSELG
                     VLSIACEGNSFPSVGHLHAPAVRLERAVRDLDGLEPEGCVDARAWLDHGKWPIRWPLG
                     QTECAAPAPGPYPFLPAEGESLHQIPVGPVHAGIIEPGHFRFTASGETIVRLEARLGY
                     VHKGIESLMAGAPIGRAAVLAGRVSGDSTVAYALAFARAIEAAHELDVPARALWLRAL
                     MAELERLANHFGDIGAVCNDAAFAMLHAHFGILREQVLRAADRCFGHRLMMDCVVPGG
                     MRCDLTGNGAQTLSGLIDHVRKQFPELIELYDNTASLQDRTVGTGILPPVLARQFGCG
                     GYVGRASGRAFDARRVPGYPPYDRLQFEVPVLEEGDVDARVWIRIREVEQSMSLIDQI
                     LRELPAGPISQPLPPPREHSREGLALVEGFRGDVLVWVKLGPDGSVERCHLRDPSWFQ
                     WPLLEAVIEGNIVADFPLCNKSFNCSYSGHDR"
     gene            5073..5615
                     /gene="nuoB"
                     /locus_tag="VD791_00680"
     CDS             5073..5615
                     /gene="nuoB"
                     /locus_tag="VD791_00680"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HXV06519.1"
                     /translation="MRRMLFKSLVHGPLTEAAPLRDDAAVAELAARVDRAARRRLGRT
                     LALREVDAGSCNGCELEIHALNNVLYDLERFGMRFVASPRHADVLLVTGPVTTNMREA
                     LERTYQATPHPKWVVAVGDCARDGGVFSGSYACVGGVSAVLSVDLHIRGCPPSPTALL
                     KGLLALLEHADREPAAGVDE"
     gene            5750..6073
                     /locus_tag="VD791_00685"
     CDS             5750..6073
                     /locus_tag="VD791_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06520.1"
                     /translation="MLVLGACASSPKPKVPAELIGDWQYIRTSAWVRIAPDGRVFQCR
                     HGRSGQLFRGMGRLNGSVIAWEQVWEPDEVARRGDALMLTGPYGSFLFGRPLDPLPAI
                     CEAPF"
     gene            6144..6620
                     /locus_tag="VD791_00690"
     CDS             6144..6620
                     /locus_tag="VD791_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013210567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV06521.1"
                     /translation="MSEATRAGDQVRIRPAQARDLPEAARLFDAYRQFYGQPADLEAA
                     QVFLCARFERRESVLLVAGAGAGLCGFTQLYPTFCSVAAAPVFVLYDLFVEPATRRCG
                     VGRALLRAACEHAQACGAVRLELATARVNAPAQALYESEGWVRDAAFYRYSLDVRR"
     gene            6854..7306
                     /locus_tag="VD791_00695"
     CDS             6854..7306
                     /locus_tag="VD791_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06522.1"
                     /translation="MDARYEQLSYLHGWWYGAADFLGSAAVRIRLPGDLGGPLPRARA
                     ALLAVDRAWDCLERQIHPYLWAAFLAACEARGARSKASRAQRDAPGERMHQRYRVAAV
                     CAGAYGEAGLVELALEPRWAPGRVLGAYVQNNMLVEFRGNIGLWRTPQ"
     gene            7343..7966
                     /locus_tag="VD791_00700"
     CDS             7343..7966
                     /locus_tag="VD791_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HXV06523.1"
                     /translation="MIAGALLTGGIVGFSIAAPVGPIGALTIRRTLAQGRTAGFLTGL
                     GAATADSAYGAVAAFGLTFVGSFLVAQQGWLSLLGGLFLCYLGVTTFRAQPAPIAHTP
                     PARSLLAAYGSTVFLTLTNPATILSFIGIYAGLGLRESRTDYTAASAFVLGVFLGSAL
                     WWLLLSFGVGTLRERLSAHGLRWINRISGAVIAIFGLMVLLRLALSA"
     gene            complement(7973..8572)
                     /gene="rdgB"
                     /locus_tag="VD791_00705"
     CDS             complement(7973..8572)
                     /gene="rdgB"
                     /locus_tag="VD791_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HXV06524.1"
                     /translation="MRRIVLASSNPGKIREIRAVLAPLTIEVVPQSRYGVPEAGEPHA
                     TFIENALAKARNACVHAGLPALADDSGICVDALGGAPGVQSARLAGEPKSDERNNQRL
                     LELLAHTEDRRAHYYCVIVLMRHAEDPQPVLAEGEWHGEILRAPRGGGGFGYDPLFLD
                     PQLGQSGAELPMAHKNRVSHRGKALAVLAQHLAGALPSA"
     gene            complement(8577..9293)
                     /gene="rph"
                     /locus_tag="VD791_00710"
     CDS             complement(8577..9293)
                     /gene="rph"
                     /locus_tag="VD791_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HXV06525.1"
                     /translation="MRPSNRAPGELRPVRLTRGYTRHAEGSVLAEFGHTRVICTASIE
                     DKVPAFLKGRAQGWVTAEYGMLPRATGTRLQREAAAGRQSGRTQEIQRLIGRSLRAVT
                     DLSAVGERTIQIDCDVIQADGGTRTAAITGAFVALHDACRVLQERQPACAWPLRDLVA
                     AVSVGIYRGVPVLDLDYDEDSACETDMNVVMTGAGALVEVQGTAEGKTFTRAELDAMV
                     DLASAGVARLIAIQRAALGL"
     gene            9454..10320
                     /locus_tag="VD791_00715"
     CDS             9454..10320
                     /locus_tag="VD791_00715"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HXV06526.1"
                     /translation="MIYSMTGFAALSEDVPLGTLSVELRSVNHRYLDLSFRLPDELRA
                     LEPGLREALSARLTRGKVECRIGLQKNPATTLSLGVNDALLQQLVELDRRVRERLPQA
                     VALSVSDVLRWPGLLAADELPLEALKDRTQALLERTLAEFTRSRAREGDKLKALLLER
                     VARMEELVARVKPKIPLLVQAYQERLSARLRDAAAELDEDRLRQELLLFSSKVDVEEE
                     LSRLVTHLAETRRILQQGGTVGKRLDFLMQELNRESNTLASKSVDIDVSQASVELKVL
                     IEQMREQVQNIE"
     gene            10470..11078
                     /gene="gmk"
                     /locus_tag="VD791_00720"
     CDS             10470..11078
                     /gene="gmk"
                     /locus_tag="VD791_00720"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HXV06527.1"
                     /translation="MTGNLFIVCAPSGAGKTSLVNALLEREPEVELSVSYTTRAPRNG
                     ETDGVDYHFVTRDVFLAMTGRGEFLESAEVHGNLYGTSQAWIDARTREGRDIVLEIDW
                     QGAQQVRRLAPSAVGVFVLPPSLEALRRRLTARGQDSSEVIEQRLANARDEIAHVEEF
                     DYVIINQNFDVAVVDLVSIVRATRLKVTAQLTRHRDLINAMK"
     gene            11086..11292
                     /gene="rpoZ"
                     /locus_tag="VD791_00725"
     CDS             11086..11292
                     /gene="rpoZ"
                     /locus_tag="VD791_00725"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HXV06528.1"
                     /translation="MARITVDDCLRLIPNRFVLTLAATYRARQLTQGASPMIEGAKDK
                     PTVTALREIAAGKVGTEILNKGQA"
     gene            11401..13518
                     /locus_tag="VD791_00730"
     CDS             11401..13518
                     /locus_tag="VD791_00730"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HXV06529.1"
                     /translation="MSEAELLIRDASSYLKPEDVRQLRSAYRFSEAAHDGQFRRSGEP
                     YISHPVAVATILVEWHLDSQALTAALLHDVMEDTTVTKGEISDKFGRMVAELVDGVSK
                     LDKIEFESQEQAQAENFRKMFLAMARDVRVILIKLADRLHNMRTLDVMQPRKRARIAR
                     ETMEIYAPIANRLGLNSVYQELQDLAFQNLYPNRYRVLRKALESQRGNRKEVVEKILD
                     AIRRQLEEAGLEAHVSGREKNLYSIYRKMQDKSLSFSKVLDIYAFRVIVRDVPACYLA
                     LGALHGLYKPIPGKFKDYIAIPKANGYQSLHTTLFGPFGSPIELQVRTQEMHTLAEAG
                     VASHWLYKSSDASLNELQRKTHQWLQSLLEMQSETGDSVEFLEHLKVDLFPDETYVFT
                     PKGKIMALPHGATCVDFAYAVHTDIGNRCVAVKVNHELMPLRTELKNGDRVEIITASH
                     AKPNPSWLNFVVTAKARHHIRHFLKTMQYEESAQLGERLLAQALVALGGSLKELREQD
                     WERLLKESAAKSKQEVLADIGLGRVLAIVIARKLLPSATGHAAPAAAASGPVVIRGSE
                     GMALQFAKCCHPIPGDPIIGFIRKGRGMIVHTHDCPVIAKMKREPDKWLDVEWAPETR
                     KLFSVNIKLLVSNQQGVLAKVASEISANNSNIENVSVEPGDSAAYSNIHFTLQVSNRM
                     HLAQIMRGLRRLPEVIRINRLKG"
     gene            13586..14239
                     /locus_tag="VD791_00735"
                     /pseudo
     CDS             13586..14239
                     /locus_tag="VD791_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869789.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
     gene            14524..14838
                     /locus_tag="VD791_00740"
     CDS             14524..14838
                     /locus_tag="VD791_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06530.1"
                     /translation="MKRVLFALLLGVWLAVPGLYAGEDDAEDAAQAAQSKVPSPLRQE
                     SLELPGLTACHQCEWRPKPNQMAAAEQCGMTPDGTPKVGLFECGFSADCERLCNFVEC
                     LK"
     gene            15075..15428
                     /locus_tag="VD791_00745"
     CDS             15075..15428
                     /locus_tag="VD791_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06531.1"
                     /translation="MRLLSGRSSKTLGQLVRSVLEDAVALGVVGFALLGVGGVAYKAL
                     SPDGWLSKAVDSLWEKSPGLLWLVAFTIAAITLVVKHHFDRDPRLTSRGNVLAYAFVA
                     LGLFFFFKLLITGSL"
     gene            complement(15587..16468)
                     /locus_tag="VD791_00750"
     CDS             complement(15587..16468)
                     /locus_tag="VD791_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXV06532.1"
                     /translation="MRLCTIRLEGEETAAVCTGEAVVPVRSINAHLGRSWPTDLLSLI
                     AQGLSPILLRDAERTPWMLPASQVACGPLYRHPRKIWGIGLNYRDHAADLSAPFPSEP
                     ASFMKGDHTIIGPGDAIQLPHESERVTAEAELGVIIGRECRFVSQDDAPAVIAGYCLI
                     LDMTAEDILQRNPRFLTRAKNFDTFFSFGPQLITPDEVLDVLRLRVGTWRNGELHREN
                     LVANMAFPPFYLVSFHSHVMTLYPGDIISTGTPGAVVIEHGDVAECRIEGIGWSLANP
                     VRRGAQADYTMLASVGV"
     gene            complement(16597..17583)
                     /gene="egtD"
                     /locus_tag="VD791_00755"
     CDS             complement(16597..17583)
                     /gene="egtD"
                     /locus_tag="VD791_00755"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HXV06533.1"
                     /translation="MRFARTPALRSTPSNLSVLDLAPRASSLLAEVLAGLGEPSKRLP
                     PKLFYDARGAELFNRICGTQAYYPTRTERAILTRHAEDVARLLGAGGVVVEPGAGDMC
                     KIRILLPALRPRAYVPIDISPDQLLAEAESLAREHRWLQVVAVSADFHARELDDRLTL
                     PSGRRTVFFPGSTIGNFDPGEALDFLRRARELAGEGGAALIGVDLDKPKPILDRAYND
                     PEGYTAQFNLNVLARINREMEADFDLDAFAHRAFYNAAHGRIEMHLVCSRRCSVSVAG
                     RRFEFDAGETIHTENSYKYAPEMFARMACAAGFSTARRWSDAAGWFGVFLFS"
BASE COUNT         2638 a         6044 c         6098 g         3019 t
ORIGIN      
        1 ccgcagctcc tacaacggcc tgctgctgtt cctcttcatc gtcgcagcgg cgtggctcgc
       61 ggcaaccgtc atccgccgct tcgcttccgg ggcgttgcgg cgtgcgccgg cgtgggactg
      121 tggctttccc gacgccagcc cgctcacgca gtacacctcg gccagcttcg cccagccgat
      181 ccggcgcgtg ttcggcagcg tcgtgttcca cgcccgcgag gaggtgcaaa taccggcccc
      241 gggacagacg ctgcccgcac gcttccacgt gtcgatgcgc gacctcgtat gggacgcggt
      301 gtatgcgccg gtcgagagcg tcgtgaactg gacctccgac cgcctcaaca aggtgcagtt
      361 ctggaccatc cgcgcgtatc tgaccctggt gttcggcgcg gtcgtgctgc tgttgatggt
      421 gctggcggta tggcattgat cctggacgcg ctggtccagc tcgcgcagct gttgctggtg
      481 ctgtcgctcg caccgttgct gaccggctat gtgcgcaagg tcaaagcgca cctgctgcgg
      541 cgccgagggc cgcctctggt ccagccctac cgggatctgc tgaagctgat ccgcaaagag
      601 gcggtgctcg cggagaacgc ttcctgggtg ttccgggtcg cgccctacct gatcttcgcc
      661 accaccttga tcgccgccgc actggtaccg accttcgcca ccggcctggt gttcagctcc
      721 gcggccgatc tcatcgccat cgtggcgctg ctgggcagcg cacgtttcgc cctggcgctg
      781 gccgggctcg atatcggcac cagcttcggc ggcatcggct cgagccgcga gatgatgatc
      841 gcgtcgctgg ccgaaccggc gatgctgatg atcgtattca ccctgtccct tctggcgggc
      901 tcgacgcagc tctcctcggt cgccgccttc atgcaagggc ccgaggtcgg cctgcgcgtg
      961 tcgctcgcca tgtcgctggt ggcgctgatc atggtggccc tggcggagaa cgccaggatc
     1021 ccggtggaca atcccgccac ccacctcgag ctgactatga tccacgaggc catgatcctc
     1081 gagtattcgg ggcgccatct ggccgtgatc gaagcggccg cctcgctcaa gctgctgctc
     1141 tacctgtctc tcatcgcttg catgttcgcg ccgtggggag tcgcgaccac cggccagggc
     1201 ttcgccgcct acgcgatcgg cctgggaacc ttcctgctca agctggcggc gggtggtttc
     1261 ctgctggcgg tgttcgagac ggtgaccgcg aagatgcgcg tgttccgggt ttcggatttc
     1321 ctcggcgcgg cgctcatgct cggcctgttg gccgcattgc tgctgttcgt atcgcagagc
     1381 gcgtgatgga ccggctcgcc ctggatgtgg cccatctgtt cgccggcggg ctggtgctgg
     1441 tgagcttcat gctgctgtat caggaccgca tgttcggcct gctcaacatc ttcgcgctgc
     1501 acgcgctggt ggtttcgctg tcggtggcgt ggcaggccta catccaggcc gcgccgcatc
     1561 tgtacatcac ggccggcatc gcgctggtgg tcaaggccat catcatcccg gtggcgctgc
     1621 accgcatcat cgtgcggctg ggcatccatc gcaccatcga aaccgtggtc ggcatcgggc
     1681 agaccatgct cgccggcatc gcgctggtgg ccttgtcgat cctggtcatg ctgccgatca
     1741 ccgccgaggc cgggacgctg gcgcgcgagg atctcgcgtt ttcgctctcc gtgatcctgc
     1801 tcgggctgct gatgatgatc tcgcggcgca acgcggtgag ccagatcatc ggcttcatgt
     1861 cgatcgagaa cggcctgatc ctggctgcca ccgccgccaa gggcatgccg ctggtggtgg
     1921 agatcagcgt ggcgttttcg atcctggtcg cgctcatcgt gatcggcatc ttcctgtttc
     1981 gcatccgcga gcgcttcgac acggtggatg tgcacgcgct ggagcgcttc cgcggagagc
     2041 agccatgatc gcgccgggcc cgctgctggt ccaggcgatc ctggtcgttc ccgcaggcag
     2101 cgctgcgttg ctggcgctgt tcccggacga tcgagccacc ggacggctca atgcggtggg
     2161 cacgttcctc acgttcctcg ccgcggccgg cctgttcttc gtccggcccg agccggggcc
     2221 gtttctgatc gtggacgacg tgaacgtcgt gttcatcgtg ctgaacacct tcgtcggctt
     2281 caccaccagc gtgttcagcg ccagctatat cgcacacgag ctcgacgtcg gccgcctgac
     2341 gccgacctat ctgcgcttct accacgccat gtatcagacg ctgatgttcg cgatgaacct
     2401 cgcgttgctg gcgaacaatg tcgggctcat gtgggtcgcc gtcgagctcg ccaccctcac
     2461 cacgatcctg atggtcggca tctaccgcac ccccgaggcg ctcgaggcgg cctggaagta
     2521 cttcatcctg ggcagcgtcg gcatcgcgct ggccctgttc gggaccatcc tcgtctatct
     2581 cgccgcacgc tcggtggtgg gcgagggtct cgacgccatg gcctggaacg tcctcgtcgg
     2641 gcaggccgcc gccttcgatc cgactctgct caacctcgcc ttcgtgttcc tgctgctggg
     2701 ctacggcacc aaggtcggac tcgccccgtt gcacgcctgg ctgcccgacg cacatgccga
     2761 aggtccaacc ccgatttccg ccgtgctgtc cggattgctg ctgaacgtcg cgctgttcgc
     2821 ggtgctgcgc ttcaagatgg tgctgtccgc caaccccgaa gcgctggcgc cggggccgct
     2881 gatggtcacg ctgggattga tctcggtggt gttcgcggcc ttcatgctct accgccgtca
     2941 cgacatcaag cgcatgttcg cctactcctc catcgagcac atggggatca tcacgttcgc
     3001 cttcggcatg ggcggcccgc tcgcgaactt cgccgggttg ctgcacatga cctttcacag
     3061 cctgaccaag tccgccatct tcttctccgt cggccacatc gcgcaagtga agggcaccca
     3121 gcgcatggcc gatattcgcg gcctcaccgt ctcgcacccg gtcctggggt ggggtctggt
     3181 gctgggcgtg atgaccatcg tgggcttacc gcccatgggt atcttcatga gcgagtttct
     3241 gatcgtcacg tccaccttcg cgcgccagcc ggtgctcgcg gtggcgctgg cggccggttt
     3301 gctggtggca ttgggagccc tgctgcttcg gctcaatggc ctcgcgttcg gcgacccgca
     3361 tggcagcacc gcgccggcgc aggcttcgtt cgtcccgatg ttcgcgcatc tggcgctggt
     3421 gctggccgcc ggaatctatc ttccggcgcc gttggtggcc tggttccggc acgtcgcggg
     3481 gctgctgggt taagccgatg gaagcggccg cgggcgcgac aaccttgcgc gacatcatcg
     3541 gagagggccg gatcgttcgg caacatcggc cctggccgcg cgcggtgatc gcccgcgaga
     3601 gctggcaacg cgccgccgga ctgctgcagg cgggtcgctg caccctgctc ggcttgtggg
     3661 gcgaacgagc tttcgtgcat ctcgcgctgg tcgacgaacg ccgcagcgaa ctcggcgttc
     3721 tgagcatcgc gtgcgaaggc aacagcttcc cgtcggtagg acatctgcat gcccccgcgg
     3781 tgcggctgga gcgggcggtc cgggacctgg acgggctcga gcccgagggg tgcgtcgacg
     3841 cgcgcgcctg gctcgatcac ggcaagtggc cgattcgctg gccgttgggg cagacggagt
     3901 gcgccgcgcc tgcgcccggg ccgtacccgt tcctgccggc cgaaggggag agcctgcacc
     3961 agatcccggt cgggccggtc cacgccggca tcatcgagcc cgggcacttc cgcttcacgg
     4021 caagcggcga gaccatcgtg cgcctggagg cacggctcgg ctacgtgcac aagggtatcg
     4081 aatcgctcat ggccggagcg ccgatcgggc gggccgcggt cctcgccggg cgtgtttcgg
     4141 gcgacagcac ggtggcctac gccctggcgt tcgcccgggc gatcgaagcg gcgcacgaac
     4201 tcgacgtccc ggcgcgcgcg ctgtggctgc gcgcgctgat ggcagagctg gagagactgg
     4261 cgaatcactt cggcgacatc ggtgccgtct gcaacgatgc cgccttcgcc atgctgcacg
     4321 cgcacttcgg cattctgcgc gagcaggtgc tgcgcgcagc cgatcgttgc ttcggccacc
     4381 ggctgatgat ggattgcgtc gtgcccggcg ggatgcgctg cgacctcacc ggcaacggag
     4441 cgcagaccct gagcgggctg atcgatcatg tgcgcaagca gtttccggaa ctgatcgagc
     4501 tgtacgacaa caccgcgtcc ctgcaggatc gcaccgtcgg cacgggcatc ctgccgccgg
     4561 tgctcgcgcg gcagttcggc tgcgggggtt acgtcggccg ggcctccggg cgggcgttcg
     4621 acgcgcgacg cgtgccgggt tacccacctt acgaccggtt gcagttcgaa gtgcccgtgc
     4681 tcgaggaagg cgacgtggac gctcgcgtgt ggatccgcat ccgcgaggtc gagcaaagca
     4741 tgtcgctcat cgatcagatc ctgcgcgagc ttcccgccgg tccgatctcg caaccgcttc
     4801 cgccgccgag ggagcacagc cgcgaagggt tggcgctggt cgagggcttc cgcggcgacg
     4861 tgctggtatg ggtgaagctc ggccccgacg gcagcgtcga gcgctgtcac ctgcgcgacc
     4921 cctcttggtt ccaatggccg ctgctggagg ccgtgatcga gggcaacatc gtcgccgact
     4981 ttcctctgtg caacaagtcc ttcaactgct cctactcggg ccacgatcgg tagcccggat
     5041 gattcacgaa cacgcccctg gggtgaagcg cgatgcgtag aatgctgttc aagagcctgg
     5101 tgcacgggcc cctcaccgag gcggcgccct tgcgggacga cgccgcggtg gccgaactcg
     5161 cggcgcgggt cgaccgcgcc gcgcgccggc ggctgggtcg aacgctggcg ctgcgcgagg
     5221 tggatgcggg ctcctgcaac ggttgcgagc tcgagataca cgccctcaac aacgtcctgt
     5281 acgatctcga gcgcttcggt atgcgtttcg tggcttcgcc gcgccacgcc gatgtgttgc
     5341 tggtgacggg cccggttacg accaacatgc gtgaggcgct cgagcgcacc taccaggcga
     5401 ccccacaccc gaaatgggtc gtcgccgtgg gggactgtgc gcgcgacggc ggggtattct
     5461 cggggagcta cgcctgcgtg ggcggcgtgt ctgcagttct gtctgtcgat ctgcacattc
     5521 gcggctgtcc cccctcgccg accgccctgc tcaaaggcct gctcgcccta ctcgagcacg
     5581 ccgatcgcga accggctgcc ggtgtcgacg aatgaaagat ccacctggag ccgccgggcg
     5641 cgcaccgacc gtgtgcgcat cagccggcgg tgcatggccc tggcatccat ggcccgggcg
     5701 tgcatggccc cggcgtgcat ggccccggcg tgcatggctg ggcgccgccg tgctggtgct
     5761 cggcgcttgc gccagttcgc cgaaacccaa ggtccccgcc gagctgatcg gcgactggca
     5821 gtacatccgc acatccgcct gggtgcgcat cgcgccggac ggtcgtgtgt tccagtgccg
     5881 tcatgggcgc agcggccaat tgttccgcgg gatggggcgc ctgaacggct cggtcatcgc
     5941 gtgggagcag gtatgggagc cggacgaggt cgcgcgccga ggcgacgcgc tcatgctcac
     6001 cgggccttat ggcagcttcc tcttcggccg cccgctcgac cccctgcccg cgatctgcga
     6061 ggcgccgttc tgaggccctg ccccgactgc ccgaagttcc ttcagatcag cccgaagttt
     6121 gttcagatca gagtggcgcg ccgatgagcg aagcgacccg ggccggggat caggttcgga
     6181 tcagaccggc gcaggcacgg gatctgcccg aggcggcgcg cctgttcgac gcctaccggc
     6241 agttctacgg ccagcctgcc gatctcgagg cggcgcaagt gttcctgtgc gcccgcttcg
     6301 agcgccgcga atccgtgctg ctggtcgccg gagccggggc cgggctgtgc ggtttcacgc
     6361 agctctatcc cacgttctgc tcggtcgcgg ccgcgcccgt cttcgtgctc tacgatctgt
     6421 tcgtggaacc cgccacacgg cggtgcggcg tgggccgcgc cctgctgcgc gccgcctgcg
     6481 agcatgcgca ggcttgcggc gccgtgcgcc tggagctcgc cacggcccgc gtcaacgcgc
     6541 ccgcccaggc cctgtacgaa tccgagggct gggtgcgcga cgcagccttc taccggtaca
     6601 gcctggacgt ccgccgctga agtctgactc gattgtcaat tccgcgtgcg cttgcaagga
     6661 ttttgctacg cgccttggcg tggacttccc caactccgca tctcacgcct cgtgcagcgc
     6721 ggcaccgccg ggcgggctgg ccgcggcggg cggtatgacg cgcgcatccg gcgactgaat
     6781 gggcccgact gcgtcccgtc atccggcgcg agcgccgcgc cgcgctgtcg aagcgcccgc
     6841 gcgcgccgta cgcatggatg cgcgctacga acagctctcc tacctgcatg gctggtggta
     6901 cggcgcggcc gacttcctcg gctccgccgc cgtgcgcatc cgcctgcccg gcgacctggg
     6961 cggccctttg ccgcgcgcgc gcgcggcgct gctcgccgtg gaccgcgcct gggattgcct
     7021 ggagcggcag atccatcctt acctctgggc ggcatttctg gccgcatgcg aggcgcgcgg
     7081 cgcccgctcg aaggcgtcgc gcgcgcagcg ggacgcgccg ggagagcgca tgcatcagcg
     7141 ctaccgcgtc gccgcagtgt gtgcgggcgc ctacggcgag gcgggtctgg tggagctggc
     7201 gctggagccg cgctgggcac cggggcgcgt gctcggcgct tacgtgcaga acaacatgct
     7261 ggtggagttc cgcgggaata tcggactctg gcgcacaccg caatagcgca gcccggcgcg
     7321 cgcgcggtgt tacactttgg ccatgatcgc gggcgcgctg ctcacgggcg gcatcgttgg
     7381 gttctccatc gccgccccgg tgggccccat cggggcgctc accatacgcc gcacgctggc
     7441 gcaagggcga accgccggct tcctcaccgg gttgggcgcg gccaccgcgg acagcgccta
     7501 cggcgcggtg gcggcgttcg gcctcacctt cgtcggcagc ttcctggtgg cccagcaagg
     7561 ttggctcagc ctgctgggcg gattgttcct gtgttacctg ggggtgacca ccttccgcgc
     7621 gcagccggcg ccgatcgcgc acactccgcc ggcgcgcagc ctgctggcgg cgtacggctc
     7681 caccgtattc ctgacgctga ccaacccggc caccatcctg tccttcatcg gcatttacgc
     7741 cgggctcggg ctgcgggaaa gtcgcacgga ctacacggcg gcgtcggctt tcgtgctcgg
     7801 cgtgttcctg ggctctgcgc tgtggtggct gctgctgagc ttcggtgtgg gcacgctgcg
     7861 cgagcggctg agcgcgcacg gcctgcgctg gatcaaccgc atctcgggcg cggtgatcgc
     7921 gatcttcggt ctgatggtgc tgctgcggct cgcgctgagc gcttgaccgc gcttacgcgc
     7981 tcggcagcgc cccggcgagg tgctgggcga gcacggccag cgccttgccg cgatggctga
     8041 cgcggttctt gtgcgccatc ggcagctccg cgccgctctg gccgagctgc gggtcgagga
     8101 acagcggatc gtacccgaag ccgccgccgc cgcgcggcgc acgcaggatc tctccgtgcc
     8161 attcgccttc cgccagcacc ggctgcggat cctcggcatg gcgcatgagc acgatcacgc
     8221 agtagtaatg ggcgcgccgg tcctcggtgt gcgcgagcag ttccaacagc cgctggttgt
     8281 tgcgctcgtc cgacttgggc tcgcccgcca agcgcgccga ctgcaccccc ggagcaccgc
     8341 ccagcgcgtc gacgcagatg cccgagtcgt cggccagcgc gggcaggccc gcatgcacgc
     8401 aggcgttgcg cgccttggcc agcgcgttct cgatgaacgt cgcgtgcggc tcgccggctt
     8461 ccggcacccc gtagcgcgac tgcggaacca cctcgatcgt gagcggcgcg agcacggcgc
     8521 ggatctcgcg gatcttgccc gggttgctgc tggccaggac gatgcgtcgc atcgcctcac
     8581 aggcccagtg cggcgcgctg aatggcgatc agccgtgcga cgcccgcgga tgcgagatcg
     8641 accatcgcgt ccaactcggc gcgggtgaag gtcttgcctt cggcggtgcc ctgcacttcg
     8701 accagggcgc ccgcgccggt catgaccacg ttcatgtccg tctcgcaggc cgagtcttcg
     8761 tcgtaatcga ggtcgagcac cggcacgccg cggtagatgc ccaccgagac cgcggcgacg
     8821 aggtcgcgca gcggccaggc gcatgccggc tgccgctcct gcagcacgcg gcaggcgtcg
     8881 tgcagagcga cgaaagcgcc ggtgatggcg gcggtgcgcg tgccgccgtc ggcctggatc
     8941 acgtcgcagt cgatctggat ggtgcgctct cccacggccg acaggtcggt gaccgcgcgc
     9001 aggctgcgcc cgattaggcg ctggatctcc tgcgtgcgcc cggactggcg gccggcggcg
     9061 gcctcgcgct gcaatcgcgt gccggtcgcg cgcggcagca tgccgtactc ggcggtgacc
     9121 cacccctgcg ccctgccctt caggaaagcc ggcaccttgt cctcgatgct ggcggtgcag
     9181 atcaccctgg tgtggccgaa ctccgccagc accgagcctt cggcgtgacg cgtgtagccg
     9241 cgcgtcagcc gcacggggcg cagttccccg ggggcacgat tgctcggtcg cataagtcgc
     9301 gtgggtgtcg ccggctcggg ccgcccgcgc tcgcgcggca gccgcggcta tgccgaaaga
     9361 ttatgtgcta tcttttagag cccactgggc tcgttccgcc cgtcatgccc gtcctgagga
     9421 agtgggctgc cacagtatag tcgaggaagg cgcgtgatct acagcatgac cggcttcgcc
     9481 gcgctgtccg aggacgtgcc gctgggcacg ctcagcgtgg agctgcggtc ggtcaaccac
     9541 cgctatctcg acctgagctt ccggctgccc gatgagctgc gcgcgctcga gccggggctg
     9601 cgcgaagcgc tgtccgcgcg cctcacccgc ggcaaggtgg aatgccggat cggtcttcag
     9661 aagaaccccg ccaccacctt gtccctgggc gtgaacgacg cgctgctgca gcagctggtg
     9721 gagctcgacc ggcgcgtgcg cgagcgcttg ccgcaggcgg tggcgttgtc ggtgagcgac
     9781 gtgctgcgct ggccgggcct gctggcggcc gacgagctgc cgctggaagc gctgaaggac
     9841 cgcacgcagg cgttgctcga gcgcacgttg gccgagttca cccgctcgcg ggcgcgcgag
     9901 ggcgacaagc tcaaggccct gctgctcgag cgtgtggcgc gcatggaaga gctggtcgcg
     9961 cgggtgaagc cgaagatccc gttgctggtg caggcctacc aggagcgcct gtccgcgcgg
    10021 ctgcgcgacg ccgccgcgga gctggacgag gaccgcctga ggcaggagct gctgctgttc
    10081 tcgtccaagg tggacgtgga ggaggagctg tcgcgcctgg tcacgcatct ggcggagacg
    10141 cgccgcatcc tgcagcaggg cggcacggtg ggcaagcgcc ttgacttcct gatgcaggag
    10201 ctcaaccgcg aatcgaacac gctcgcctcg aagtcggtgg acatcgacgt gtcgcaggcc
    10261 tcggtggagc tgaaggtgct gatcgaacag atgcgcgagc aggtgcagaa catagaatga
    10321 ggcgcgcggc acgagggaaa cccgatggtg gtgcggccac tcggcgtcgc cgccggacga
    10381 gcggctcatc aagttgtcgc gtatcaagcc gttacctatg atgtgattcg ccctgccaac
    10441 gcgccctcgc gcctcgcgcc tgaattcaag tgaccggaaa tctgttcatc gtctgcgccc
    10501 cttccggtgc gggcaagacc agcctggtga atgcgctgct cgagcgcgag cccgaggtcg
    10561 agctgtcggt gtcctatacc acgcgcgcac cgcgcaacgg cgagaccgac ggggtcgatt
    10621 accatttcgt gacgcgcgac gttttcctgg cgatgaccgg tcgcggcgag ttcctggaaa
    10681 gcgccgaggt gcacggcaac ctgtacggca cctcgcaggc ctggatcgac gcacgcacgc
    10741 gggaagggcg cgacatcgtg ctcgaaatcg actggcaggg agcgcagcag gtgcgccgcc
    10801 tggccccctc ggcggtgggg gtgttcgtcc tgccgccgtc gctggaggcg ctgcgccgcc
    10861 gcctgaccgc ccgcgggcag gattcctcgg aggtgatcga acagcgcctg gccaacgccc
    10921 gcgacgagat cgcccatgtc gaggagttcg actatgttat tatcaatcag aattttgacg
    10981 tggcagtagt ggatctggtc agcatcgtgc gcgccacgcg cctgaaagtg accgcccagc
    11041 tcacgcgcca ccgcgatctg atcaatgcga tgaaatgagg ttgccatggc ccgcatcacc
    11101 gtggacgact gcctgaggct catccccaac cgcttcgtgc tcacgctcgc cgcgacctac
    11161 cgcgcgcgcc agctcaccca gggcgcctcg ccgatgatcg agggggcgaa ggacaagccg
    11221 accgtgaccg cgctgcgcga gatcgccgcc ggcaaagtcg ggaccgagat cctcaacaag
    11281 ggccaggcct agcgcccctt gccccgcgtc cgcccacgtt ccccgatccc gcatcgagcc
    11341 gtcgaaacgc cgcagacaac gctgccccga gggctcgcgg accgaaggtg tgacccgacg
    11401 atgtccgagg ccgagcttct gattcgcgat gcttccagct acttgaagcc ggaggacgtg
    11461 cgccagctgc gatcggccta ccgcttctct gaggccgccc acgacgggca gttccgccgc
    11521 tccggcgagc cgtacatctc gcatccggtg gccgtggcca ccatcctggt cgagtggcac
    11581 ctggattccc aggcgctcac tgccgccctg ctgcacgacg tgatggagga caccaccgtc
    11641 accaagggcg agatcagcga caagttcggg cgcatggtgg ccgagctggt ggacggcgtg
    11701 tcgaagctgg acaagatcga gttcgagagc caggagcagg cgcaggcgga gaacttccgc
    11761 aagatgttcc tggcgatggc gcgcgacgtg cgcgtgatcc tgatcaagct cgccgaccgc
    11821 ctgcacaaca tgcgcaccct ggacgtgatg cagcccagga agcgcgcgcg catcgcgcgc
    11881 gagacgatgg agatctacgc gccgatcgcc aaccgcctgg ggctgaacag cgtgtaccag
    11941 gagctgcagg acctcgcctt ccagaacctc tacccgaacc gctaccgggt gctgaggaag
    12001 gccctcgagt cgcagcgcgg caaccgcaag gaggtggtcg agaagatcct ggacgcgatc
    12061 cggcgccagc tggaagaggc gggcctcgag gcgcacgtga gcggtcgcga gaagaacctg
    12121 tacagcatct accgcaagat gcaggacaag agcctgtcct tctccaaggt gctggacatc
    12181 tacgcgtttc gcgtcatcgt gcgcgacgtg ccggcctgct acctggcgct gggtgcgctg
    12241 cacgggctgt acaagccgat ccccggcaag ttcaaggact acatcgcgat cccgaaggcc
    12301 aacggctacc agtcgctgca caccacgctg ttcggcccct tcggcagccc gatcgagctg
    12361 caggtgcgca cccaggagat gcacacactg gcggaggccg gcgtggcctc gcactggctg
    12421 tacaagagca gcgacgcctc gctcaacgag ctgcagcgca agactcacca gtggctgcag
    12481 agcctgctcg agatgcagtc cgagaccggc gactcggtgg agttcctcga gcacctgaag
    12541 gtcgatctgt tcccggacga gacctacgtg ttcacaccca agggcaagat catggcgctg
    12601 ccccacggcg cgacctgcgt ggacttcgcc tacgcggtgc acaccgacat cggcaaccgc
    12661 tgcgtggcgg tcaaggtcaa ccacgagctg atgccgctgc gcacggagct gaagaacggc
    12721 gaccgcgtgg agatcatcac ggccagccat gccaagccca atccgtcctg gctgaacttc
    12781 gtggtcaccg ccaaggcgcg ccaccacatc cgccacttcc tgaagaccat gcagtacgag
    12841 gagtcggcgc agctcggcga acggctcctg gcgcaggcgc tggtcgcact gggcggcagc
    12901 ctgaaggaac tgcgcgagca ggactgggag cggctgctca aggagagcgc cgccaagtcc
    12961 aagcaggagg tcctggccga catcgggttg ggacgcgtgc tcgccatcgt gatcgcgcgc
    13021 aagctgctgc ccagcgcgac cgggcacgcc gccccggccg cggccgcctc cggcccggtg
    13081 gtgatccgcg gctcggaagg catggcgctg cagttcgcca agtgctgtca cccgatcccc
    13141 ggcgacccga tcatcgggtt catccgcaag ggtcgcggca tgatcgtgca cacccacgat
    13201 tgcccggtga tcgccaagat gaagcgcgaa ccggacaagt ggctcgacgt ggaatgggcg
    13261 ccggagacgc gcaagctgtt ctcggtcaac atcaagctgc tggtgtccaa ccagcagggg
    13321 gtgctggcga aagtcgcttc cgagatctcc gccaacaact ccaacatcga aaacgtcagc
    13381 gtcgagcccg gcgacagcgc cgcctacagc aacatccact tcaccctcca ggtcagcaac
    13441 cgcatgcacc tcgcgcagat catgcgtggg ctgcggcggc tgcccgaagt aataagaatc
    13501 aaccgactga aaggataggc gcgggcgccg ggcgaggcgc tcgatgcgtc gagcagcccg
    13561 ctgcgcgcag cgcgccgcat gccgcatgca accgttctcc cccgagttct tctccgctct
    13621 gctggccatc atcatcatcg acctggtgct ggccggggac aacgccatcg tgatcgcgct
    13681 ggccgcgcgc aagctgccgc agcacctgca gatgaaagcg gtgatgtggg gcacggccgg
    13741 cgccatcggc gtgcgcgtcg cgatgaccct gatcgtggtg gcgctgctca agattcccgg
    13801 gctgatgctg gccgggggtg tgctgctggt ctggatcgct tacaagctgc tcgcctccgg
    13861 agagcgggaa gggcagggcc acgcaggcgg acgcgagatc accacgctgt ggtcggcgct
    13921 gcgcaccatc atcatcgccg atgccgtgat ggggctggac aacgtgctcg gcgtggccgg
    13981 cgccgcgcaa ggcagcttcg cgctggtggt actgggactg ctgatcagca tcccgatcat
    14041 ggtgtggggc agcacgttga tcctgcgcat ggtggaccgc catccggtca tcgtgtacat
    14101 cggcgccgcc gtcctggctt ggacggcggc gaagatgatc ctgcacgagc cgctggtcgc
    14161 ctcgcgcacc gagcagctgc cctggctggc atggctgctc tatgcggttg tcatcggcgg
    14221 cgtcctgttg gcggggtgga aacgcaatcg cggcctcccg cacgaaggca tcatgctgcc
    14281 cgaggaggtt gcgaccccgg acgggccgcc ggagtcggcg ggtccggcgc gccaagacca
    14341 gtctccgtca caacgggatc aggctccgtc acaacgggat cagtttgcgt cacgacggga
    14401 ccaacccgaa tcggcgaggg atcgatccgc atcacaacgc gattgatctg tatcaagccc
    14461 ggaccttgct tggaccggaa tgatgggcgt gcgatactgc gcgctggcgc ggcggatgga
    14521 atgatgaaac gagtcctatt tgctctgctg ctcggggtct ggctggctgt tccggggctg
    14581 tacgccggcg aggacgacgc cgaggatgcg gcgcaggcgg cccaaagcaa ggtcccgagc
    14641 ccgctgcgcc aggagagcct ggagctgccc ggcctcaccg catgccacca gtgcgaatgg
    14701 cggccgaagc cgaatcagat ggcggccgcc gaacaatgcg gcatgacccc ggacggcacc
    14761 ccgaaggtcg gcttgttcga gtgcggcttt tctgcggatt gcgagcggct gtgcaatttc
    14821 gtggagtgcc tcaagtaggt ccccggtccc ccgtctcgcc cgcgcacccc gatcacgcct
    14881 gaagcgtggg ggcgcagggg cgcgctcctg ttctcgcctc gcgccggggc acagtcgccc
    14941 accgactacg gattgccccg ccgcgggccg tcccctggcc tgggcatttt gcgctaaccc
    15001 actgggtaca cagctgcttt ttgggctctg gtacggccgt tgcactggct ggtgagggtc
    15061 ggcggcgcca cggcatgcgg ttactatcgg gcaggtcatc aaaaaccctc gggcaactcg
    15121 tccgcagcgt actggaggac gcggtcgcgc tgggggtggt gggcttcgcg ctgctgggcg
    15181 tgggcggggt cgcctataag gcgctcagcc ccgacggttg gctcagcaag gcggtggaca
    15241 gcctgtggga gaagagccca ggcctgctgt ggctggtggc gttcaccatc gccgccatca
    15301 cgctcgtggt gaagcaccac ttcgaccgcg acccgcgcct gacctcgcgc ggcaacgtcc
    15361 tcgcgtacgc cttcgtcgcc ctcggcttgt tcttcttctt caagctcttg atcaccggat
    15421 cgctgtaatt gtctcctttc ggagacagag aaaacctcga cggtgtaagg acttacacgg
    15481 cgcggccggg ggcggctgtc gcatcccggc tacaacccgg cggattcggc tcgctggatc
    15541 atgcggttgc acgcgcccgg gcggcgccag ccggttcgat cgacctctac acgccgaccg
    15601 atgcgagcat ggtgtagtcg gcctgcgccc cgcggcgcac cgggttggcg agggaccacc
    15661 cgatgccttc gatgcggcat tcggccacgt cgccatgctc gatgaccacc gccccgggcg
    15721 tgccggtgct gatgatgtcg cccgggtaca gggtcatgac gtgagaatgg aaggacacca
    15781 ggtagaacgg cgggaaggcc atgttggcga ccagattctc gcgatgcagc tcgccgttgc
    15841 gccaggtgcc cacccgcagg cgcagcacgt cgagcacttc gtcgggggtg atgagctgcg
    15901 gcccgaacga gaagaacgtg tcgaagttct tcgcgcgcgt gaggaagcgc gggttgcgct
    15961 gcaggatgtc ctccgcggtc atgtcgagga tcaggcagta gccggcgatc accgcaggcg
    16021 catcgtcctg cgacacaaac cggcactcgc gcccgatgat cacgcccagc tcggcttcgg
    16081 cggtaacgcg ctccgactcg tgcggcagct ggatcgcgtc ccccggcccg atgatggtgt
    16141 gatcgccctt catgaaggac gccggctcgc tgggaaaggg cgccgacagg tcggccgcgt
    16201 ggtcgcggta gttcaggccg ataccccaga tcttgcgcgg gtgccggtag agcgggccgc
    16261 aggcgacttg tgacgcgggc agcatccagg gcgtgcgctc ggcgtcgcgc agcagaatcg
    16321 ggctcaagcc ctgcgcaatc agcgacagca ggtcggtcgg ccagctgcgg ccgaggtggg
    16381 cgttgatgct gcgcacgggg acgacggctt cgccggtgca gacggcggcg gtttcttccc
    16441 cctcgagccg gatggtgcaa agacgcatgt gctctcctcg ccgccggcgt cgatcgtccg
    16501 cgatggaccg tgcgcctatc ttaccccgcc cgccgtgccg ggcccgatga tcgcgagaag
    16561 gagaaccggg ggatcacgag aacaagaacc gggcgatcag ctgaacagga acacgccgaa
    16621 ccacccggcg gcatccgacc agcgccgcgc ggtcgagaag ccggccgcgc acgccatccg
    16681 cgcgaacatc tccggcgcgt acttgtagga attctcggtg tggatggtct cgccggcgtc
    16741 gaactcgaag cgccggccgg cgacgctgac gctgcagcgg cgactgcaga ccagatgcat
    16801 ctcgatgcgg ccgtgcgccg cgttgtagaa ggcgcggtgg gcgaacgcgt cgaggtcgaa
    16861 gtcggcctcc atctcgcggt tgatgcgcgc gagtacgttc aggttgaatt gcgcggtgta
    16921 gccttccggg tcgttgtagg cgcgatccag gatcggcttg ggcttgtcca agtccacgcc
    16981 gatcagcgcc gctccgccct cgcccgccag ctcgcgcgcc cgacgcagga agtccagcgc
    17041 ctcgccggga tcgaagttgc cgatggtcga acccggaaag aacaccgtcc ggcggcccga
    17101 gggaagcgtc agtctgtcgt cgagctcgcg cgcgtggaaa tccgcgctca ccgcgaccac
    17161 ttgcagccaa cggtgctcgc gcgccaggct ctccgcctcc gccagcagct gatcgggtga
    17221 gatgtcgatc ggaacatacg cgcgcgggcg cagcgccggc agcaggatgc ggatcttgca
    17281 catgtcgccg gcgcccggtt ccaccaccac accgcccgcg cccaggaggc gcgcgacgtc
    17341 ctcggcgtgg cgcgtcagga tggcgcgctc ggtccgggtc gggtaatagg cctgcgtgcc
    17401 gcagatgcgg ttgaacagct cggcgccgcg cgcatcgtag aacagcttgg gcggcagccg
    17461 tttgctcggt tcaccgagac ccgccaggac ctccgccagc agcgaggagg cgcgcggcgc
    17521 caggtcgagc accgagaggt tgctcggcgt ggagcgcagc gctggagtcc tggcgaatcg
    17581 catcgcacac cgtgatcggg acagagggag cgtgcacttg ctgcacgcgc ggccggcaca
    17641 tggcggacca ttgtatgcgc attggtcgag gcgcgccgag cgccctgaca cggccggcca
    17701 gccgctcgcg ccacgagcct gaaccgtgcc gcgtcacgct gatcacggcc gcttccaggg
    17761 cggtcgcaat gatcactccg ggcccgggcg ccagttcga
//
LOCUS       DASPGE010000027         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_593256, whole genome shotgun sequence.
ACCESSION   DASPGE010000027 DASPGE010000000
VERSION     DASPGE010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593256"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VD791_00760"
     CDS             complement(<1..309)
                     /locus_tag="VD791_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HXV06534.1"
                     /translation="MKHSLQLKLSQHLTLTPQLQQSIRLLQLSTLELNQEIERFLQDN
                     PLLERDEGAEEEGNAAQAPAAAPVTAEPTTPATTSTTTSTTSQEESEQSHAAEPEQDW
                     I"
     gene            complement(369..1148)
                     /gene="lptB"
                     /locus_tag="VD791_00765"
     CDS             complement(369..1148)
                     /gene="lptB"
                     /locus_tag="VD791_00765"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HXV06535.1"
                     /translation="MTSVTDPFPQVQPAPAAEPRSELRAEELHKRFKARTVVRDVSLA
                     VSSGEVVGLLGPNGAGKTTCFYMIVGLIPSDGGRVVLDQKNLNHMPIHMRARMGVAYL
                     PQEASIFRKLTVAENVQAVLELQLRDREQIEAQLESLLNDLHIAHLRNSPAVSLSGGE
                     RRRAEIARALASHPRFILLDEPFAGVDPIAVLDIQRIIRFLKERQIGVLITDHNVRET
                     LGICDRAYIINDGSVLACGAPEEIVYNESVRKVYLGEHFRL"
     gene            complement(1145..1765)
                     /gene="lptA"
                     /locus_tag="VD791_00770"
     CDS             complement(1145..1765)
                     /gene="lptA"
                     /locus_tag="VD791_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HXV06536.1"
                     /translation="MHRLRPAPLPRTIAALLAAALAGAPLAHAERGDREKPVNIEADR
                     MTVDDLKKESVFEGNVTLSQGTLMLRADRVVVKQDAAGFNFAYAYGKPAHFRQKRDGV
                     DEYVEGFAERMEYDGKQDKVQMFTNAHVKKGLDEVRGDYISYDAITEFYQVVGGGKQA
                     ATSTNPQGRVRAVIQPKQRDKPPAAPPTGAGAEAAPGAENVKKPAQ"
     gene            complement(1777..2379)
                     /gene="lptC"
                     /locus_tag="VD791_00775"
     CDS             complement(1777..2379)
                     /gene="lptC"
                     /locus_tag="VD791_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HXV06537.1"
                     /translation="MRDRWLAYIPALLLAGLALLTYWLDQRVQPLGPARDGGLPNPPD
                     FIVENFQAMRMHPDGTPRYLVAAKKMLHYPDDNSAVLEQPQLTHFEPDKAPVSIRANE
                     GRLSPNGEDAIFSGDVLVRRAAYDGSDELTLSTSWLHVIPEQDLAKTDREVIMTSGNS
                     TLKSVGLEFNNATRTLKLLSKVNGTFATPPKSELPWQQRR"
BASE COUNT          343 a          841 c          877 g          479 t
ORIGIN      
        1 aatccagtcc tgctcgggtt cggcggcgtg gctctgctcg ctctcttcct gggaagtggt
       61 gctggtggtg gtgctggttg tggcgggggt cgtcggctcg gcggtgaccg gcgccgccgc
      121 cggcgcctgc gcggcgttgc cttcttcctc cgcaccctcg tcgcgctcga gcaggggatt
      181 gtcctgcagg aagcgctcga tctcctggtt cagctcaagg gtggaaagct gcagcagccg
      241 gatggactgc tggagctgcg gggtgagcgt gaggtgctgc gacagcttga gctgaagaga
      301 atgcttcatt aacggatttg cgatgcggag aagccgtgcc gaaggcggcg gtcttgtcgg
      361 ggctgtcgct agagccggaa atgctcaccc agatagacct ttctcacgct ttcattataa
      421 acgatctctt cgggcgctcc gcaagcaagc accgagccat cgttgataat gtaggcgcgg
      481 tcgcagatgc ccagggtttc gcgcacgttg tggtcggtga tcagcacgcc gatctgcctc
      541 tccttcaaga accggatgat ccgctggatg tccagcactg cgatcgggtc gaccccggcg
      601 aagggctcgt ccagcaggat gaatcgcggg tggctggcga gcgcccgcgc gatctccgcc
      661 cgccgccgct ccccgcccga caggctcacc gccgggctgt tgcgcaggtg agcgatgtgc
      721 aagtcgttca gcagcgactc gagctgcgcc tcgatctgct cgcggtcgcg cagctgcagc
      781 tcgagcacgg cctggacgtt ctccgccacc gtcagcttgc ggaagatcga ggcctcctgc
      841 ggcaggtagg ccacgcccat ccgcgcccgc atatgtatcg gcatgtggtt gagattcttt
      901 tggtccaaga cgacgcgccc gccgtcgctg gggatcaggc ccacgatcat gtagaagcag
      961 gtggtcttgc cggccccgtt gggccccagc aggcccacca cctcgccgct gctgaccgcc
     1021 agggagacgt cccgcaccac cgtgcgcgcc ttgaagcgct tgtgcagctc ctcggcccgc
     1081 agctcgctgc gcggttcggc ggccggggcc ggttgcacct gcgggaacgg gtcggtaacg
     1141 ctggtcattg ggcgggtttt ttcacattct cggcgcccgg cgccgcttcc gcacccgcgc
     1201 ccgtgggcgg ggccgccggc ggtttgtcgc gctgcttggg ctggatcacc gcccgcaccc
     1261 gtccctgcgg gttggtgctc gtggcggcct gcttgccgcc gcctacgacc tggtagaact
     1321 cggtgatcgc atcgtaggag atgtaatccc cgcgcacctc gtccagaccc ttcttgacgt
     1381 gggcgttggt gaacatctgc accttgtcct gcttgccgtc gtactccatg cgctcggcga
     1441 agccctccac gtattcgtcc acgccatcgc gtttctgccg gaagtgggcc ggcttgccgt
     1501 aggcgtaagc gaagttgaag cccgcggcgt cctgcttcac caccacgcgg tcggcgcgca
     1561 gcatgagcgt gccctgggag agcgtgacgt tgccctcgaa aacgctctcc ttcttgagat
     1621 cgtccacggt catgcggtcg gcctcgatgt tgaccggctt ctcgcggtcg ccgcgctcgg
     1681 cgtgcgccag cggcgcgccg gccagcgcgg cggcgaggag cgccgcgatc gtgcgcggca
     1741 gcggggcggg gcggagtcga tgcatggcgg ggggagctag cggcgttgct gccacggcag
     1801 ctcgctcttt ggcggcgttg cgaaggtgcc gttgaccttc gacagcagct tcagcgtgcg
     1861 cgtcgcattg ttgaattcta ggccaaccga cttcagcgtg ctgttgccgc tggtcatgat
     1921 gacttcgcgg tcggtcttgg ccaggtcctg ttccgggatc acgtgcagcc agctggtcga
     1981 cagagtgagc tcgtcgctcc cgtcgtaggc cgcgcgccgc accagcacgt cgccgctgaa
     2041 gatggcgtcc tcgccgttgg gcgacagcct gccctcgttg gcgcggatcg ataccggcgc
     2101 cttgtccggc tcgaagtggg tgagctgggg ctgctccagc accgcgctgt tgtcgtcggg
     2161 gtagtgcagc atcttcttcg cggccaccag gtagcgcggg gtgccgtccg ggtgcatgcg
     2221 catcgcctgg aagttctcga cgatgaagtc gggcgggttc ggcagcccgc cgtcgcgcgc
     2281 cggcccgagc ggctgcaccc gctgatcgag ccagtacgtg agcagggcca gtccggcgag
     2341 cagcagcgca gggatgtacg cgagccagcg gtctctcatc tcagatagtc gtcgatcgcg
     2401 cgcgccaacg taccctgcgc ctgcatcagc agctcgcaca cctcgcgcac cgcaccgcgt
     2461 ccggctgcgc ggcgcgtgac gtaatgcgcg cgggcgcgca ccgcttcggg cgcctcgggc
     2521 acggcaacgg ccaggccgca
//
LOCUS       DASPGE010000028        11322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_476361, whole genome shotgun sequence.
ACCESSION   DASPGE010000028 DASPGE010000000
VERSION     DASPGE010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11322
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476361"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VD791_00780"
     CDS             <1..464
                     /locus_tag="VD791_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXV06538.1"
                     /translation="WCKRYPIVSIEDPLAEDDAAGMGLFTAAMGDRIQIIGDDYLVTS
                     AALVQKAAAERACNAVLIKPNQAGTLTETHAALRAGQAAGFGTIVSARSGETEDVTIA
                     HLAVGWDAGQLKVGSFARSERMAKWNEVLRIEEALGPAARFAGGAALHRDA"
     gene            479..946
                     /locus_tag="VD791_00785"
     CDS             479..946
                     /locus_tag="VD791_00785"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXV06539.1"
                     /translation="MNSDGGYVADDTTSKPVPEARAAYRHFQRIPTRWMDNDMYGHVN
                     NVVYYSYFDTVVNQYLIEQGALDIERSPVIGLVVETRCQYFAPITFPDRVDAGLRVAK
                     LGNSSVRYEIGLFRNDEDRASAQGHFIHVYVERASRRPAALPSQMRAALARIA"
     gene            complement(956..1537)
                     /locus_tag="VD791_00790"
     CDS             complement(956..1537)
                     /locus_tag="VD791_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HXV06540.1"
                     /translation="MLRAPTVIAILAALAACAPAPQRQAPAAKPAAPAPAPEAAEPRA
                     AAGVTVSGIEQQVIARTNAFRRQNALAALEANARLTAIAQSHVRNMARQDKFGDTDQN
                     GHVLDGADMEDRIRAGGYAFALVAENVGYQLNRGEPVAAMMDGWKNSPGHRRNLLLAQ
                     VTEIGVGAAKGRSGRWYFVQLFGRPPQVTKISQ"
     gene            complement(1588..2337)
                     /locus_tag="VD791_00795"
     CDS             complement(1588..2337)
                     /locus_tag="VD791_00795"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HXV06541.1"
                     /translation="MNCALVTGGSRGIGRAIVLTLVEAGMRVTFSYRSRAGEAEQVVA
                     AAAAQGGQAQAVRADAGRADECQRLANAALEAMGRIDVLVNNAGTHLPGAWLRDMPAG
                     EWDRILRINLDGPFHLTRAVLPHMRSRGSGHIVNISSNVTNRMPVGYGAYAVSKAALE
                     AFTRILAKEEGPAGIRVNAVAPGPIRTEMLNETLEKLGAERAEALLKTVPLGRAGQPE
                     EIAAMVAFLVSESASYVTGQVIYVNGGGTGG"
     gene            2521..3510
                     /locus_tag="VD791_00800"
     CDS             2521..3510
                     /locus_tag="VD791_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HXV06542.1"
                     /translation="MQVVEITRPGGPEVLKPATRPVPQPRAGEVLIQVAAAGVNRPDC
                     LQRAGSYAPPPGASDLPGLEVAGTVVRVGEGASEWNVGDRVCALTPGGGYAQYCLTPG
                     AHCLPPARGLDLIQAASLPETFFTVWINVFERARLAPGETLLVQGGASGIGVAAIQMA
                     RAFGHRVFATAGSAEKCAACQRLGAERAINYRTQDFVEAVQQATGGKGVDVILDMVGG
                     DYVPRELKCLANDGRLSIIAFLGGTKATLDLSDILFRRLTITGSTLRPRSVEYKAGVA
                     QALRQRVWPLIEAGAIRPVIYRTFPLAQAAQAHALMESGAHVGKIVLEVMREG"
     gene            complement(3522..4472)
                     /locus_tag="VD791_00805"
     CDS             complement(3522..4472)
                     /locus_tag="VD791_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06543.1"
                     /translation="MDPRRSEYDVSNRIDTTDPASVEGEVRRLHADLYPRAQTSTLSR
                     AFDDCTRMYRGAHPAYRACDTAYHDLQHTLDVSLAMARLMDGYQRSASRAEPIGERLF
                     VFGIVAALFHDIGYLRRRNDTRHQNGAEYTLRHVSRGGRFLHRYLADIGMEDLAAVGA
                     ELIHFTGYEMPVSAIRVPSPLFRRIGNMLGTADIIAQMSDRCYLEKCRDRLYPEFVVG
                     GLASRGGAESAPGAQFSSAEDLLNKTPAFYLSAERRLGELLGGAVDYARTHFGGRNLY
                     LDAVQKNVSHARRVAQLGEARGLLRRNPPARADQAVRLAA"
     gene            complement(4731..5267)
                     /locus_tag="VD791_00810"
                     /pseudo
     CDS             complement(4731..5267)
                     /locus_tag="VD791_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051840.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
     gene            complement(5377..6174)
                     /locus_tag="VD791_00815"
     CDS             complement(5377..6174)
                     /locus_tag="VD791_00815"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HXV06544.1"
                     /translation="MGMRIGLLSRLRRRSAGGDAQVQISEQDGVRYLHLGTDTVQSAM
                     RLSDPHELVLSYTRSMLAFLLFAPNPHRIVNIGLGGGSLAKWIHRHLTRAQQLVLELN
                     PRVIACARQYFHLPGDDARLRVLEADGAAWAAQHPGCCDLVLVDGYDGRSHAAELATR
                     EFYASTARCLTADGILVVNLWGSDRRFDETLQRIEAAFEGRICCVPAAQRGNVAVLAF
                     RRTPLATRWDELRTAASALQARYGIEFGDFVGVMRRLNPHTSRRLLI"
     gene            6255..7964
                     /locus_tag="VD791_00820"
     CDS             6255..7964
                     /locus_tag="VD791_00820"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HXV06545.1"
                     /translation="MRVSAFFLSTLKEAPAEAELPSHKLMLRAGLIRKLGSGLYTWMP
                     LGLRVLRGVERIVREEMDRAGAVELFMPAVQPAELWQESGRWDKFGPQMLKIKDRHER
                     DFCFGPTHEEVITDIARREIRSYRQLPVNFYQIQTKFRDEIRPRFGVMRAREFVMKDA
                     YSFHLDHADLEREYRNMHAAYSRIFERLGLKFRAVAADPGAIGGTGSHEFHVLADSGE
                     DAIAFSPESDYAANLELAEALPPRTPRPAPAQKMRKLATPGQHTIEQVSAFLKVPAER
                     IVKTLMVVTGDQQEYLLLLRGDHMLNEVKAGKKFLLNDGFRFASEGEVRARLRAAPGS
                     IGPVGDHQLPVIADRALQAMSDFVCGANEDDHHLLGVNFGRDLPEPEEYIDLRNVVEG
                     DPSPDGKGRLSICRGIEVGHIFQLRTKYSEAMSATVLDEAGQPRPMEMGCYGIGVSRI
                     VAAAIEQNHDERGILWPSPMAPFRIAIAPVGYGKSAPVKALADRLHDELTAAGVEALL
                     DDRDERPGVMFADLELIGIPHRITIGERGLKQGQVEYVSRREGTPRMIAADQALEFAK
                     TLG"
     gene            8088..8735
                     /locus_tag="VD791_00825"
     CDS             8088..8735
                     /locus_tag="VD791_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HXV06546.1"
                     /translation="MSLCGAVVLALWHAPAFAGAQRYEPLATSVRAALHSAVADRASP
                     RLVFATPDEGYQWLDEMSIRLVRRVPDQQTRLDLLTTVQYEAKRAGLDPQLVLGLIEV
                     ESGFRKYAVSRAGARGYMQVMPFWLNLIGTGDHNLFNLRTNLRYGCVILRHYLDVERG
                     DMTRALARYNGSLGKKSNYPGKVLRAWETRWGWPGTGVRQISGKRPAIRYMGEPD"
     gene            complement(8745..9995)
                     /locus_tag="VD791_00830"
     CDS             complement(8745..9995)
                     /locus_tag="VD791_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HXV06547.1"
                     /translation="MRRQLRAFADFLHWLHLRLREDRLLQVSGALTFTSLLALVPVVT
                     IALTVFSAFPAFGGFWSVVRNFMLTHLVPAAASKLTGVYVQQFAENAGRLTALGMAML
                     GAAAIMMMLTIEHTFNRIWRVRLARPVVARVLIYWGVLTVGPLLLGASLSLTSWLITQ
                     STGVIGKLRGPEAVILKVVPLALTCLTFAFLYRTVPYRRVQTTDAVVGGAVAGLLFEL
                     MKGLFGAYIKQVPTYKLVYGAFASFPIFLTWIYVSWLIVLIGAELTAAMPYLRTGGVR
                     LRYLPGSHLLDAVRVLRLLYEAHNRGAVLTVGDLRAALRTPWEECEALLERLAKLGWV
                     VPAAGERWVLARDVGKIRLAEVYREFVFDAEAQVRVGEEGYEAAVARLAADVQEDLSM
                     TLESLFEERGAGRGAAATKRARAA"
     gene            10102..10695
                     /gene="wrbA"
                     /locus_tag="VD791_00835"
     CDS             10102..10695
                     /gene="wrbA"
                     /locus_tag="VD791_00835"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HXV06548.1"
                     /translation="MPEILVLYYSRTGAVRQMAHMVARGIDAVPGAAARIRTVPAVSA
                     VSEAVEPPVPDAGAPYAQLADLEACAGLALGSPTRFGNMAAPLKYFLDSTSGLWLRGA
                     LAGKPAALFTSTGTLHGGQEATLLSMMLPLFHHGMLLLGLPYTEPELTTTTSGGTPYG
                     ASHVAGAAADRAVTDEEQRMCFALGRRLATVAAKLAR"
     gene            10706..>11322
                     /locus_tag="VD791_00840"
     CDS             10706..>11322
                     /locus_tag="VD791_00840"
                     /inference="COORDINATES: protein motif:HMM:NF025252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HXV06549.1"
                     /translation="MPRARAARMLVRSHRSGVLSSQSLKFPGYPYGSALPHVTDHGGR
                     PVVLISHLAEHTQNLEVDARASFIVCASGADLQAHPRATVLGAARAVVDAAPVARRYL
                     RYYPDHAQHLEIGGFRFFALEPFQVRFIQGFGGLHWIAGERYLAPEAMREAEEDILAH
                     MNRDHGEALRAYCRHVHGVEVETAEMVGCDCDGFDVRAGERLLRF"
BASE COUNT         1777 a         3919 c         3987 g         1639 t
ORIGIN      
        1 actggtgcaa gcgctatccg atcgtctcga tcgaggaccc gctggccgag gacgacgccg
       61 cgggcatggg gttgttcacc gcggccatgg gcgaccgcat ccagatcatc ggcgacgact
      121 acctggtcac cagcgccgcg ctggtgcaga aggcggcggc ggaacgcgcc tgcaacgcgg
      181 tgctgatcaa gcccaaccag gcgggcacgc tcaccgaaac gcacgccgcg ctgcgcgccg
      241 gccaggcggc ggggttcggc accatcgtgt cggcgcgctc gggcgaaacc gaggacgtga
      301 ccatcgcgca cctcgcagtc gggtgggatg cgggtcagct gaaggtgggt tcgttcgccc
      361 gctcggagcg catggccaag tggaacgagg tgctgcgcat cgaggaagcg ctgggcccgg
      421 cggcgcgctt cgcgggcggc gccgcgttgc atcgtgatgc gtgacacgcg atgcgtgatt
      481 gaacagcgac gggggatacg tggcggacga tacgacgagc aagccggttc ccgaagcgcg
      541 tgccgcctac cgacacttcc agcgcattcc cacccgctgg atggacaacg acatgtacgg
      601 acatgtgaac aacgtcgtgt actactccta tttcgacacg gtggtgaacc agtatctgat
      661 cgagcaggga gcgctggaca tcgagcgcag cccggtgatc ggcctggtgg tggaaacgcg
      721 ctgccagtac ttcgccccga tcacctttcc cgaccgggtc gatgccggcc tgcgcgtggc
      781 gaagctcggc aacagcagcg tgcgctacga gatcggcctg ttccgcaacg acgaagaccg
      841 cgccagcgcg cagggacact tcatccacgt gtacgtggag cgtgcgagcc gccggccggc
      901 ggcgctgccg tcgcagatgc gcgcggcgct ggcgcggatt gcgtagcgag cggcgctact
      961 gcgagatctt ggttacctgc ggcgggcgcc cgaacagctg cacgaagtac cagcgcccgg
     1021 acctgccctt ggcggcgccc acgccgatct cggtgacctg cgcgagcagc aggttgcggc
     1081 ggtggccggg cgagttcttc cacccgtcca tcatcgccgc gaccggttcg ccgcggttga
     1141 gctggtagcc gacgttctcg gcgacgagcg cgaacgcgta gccgccggcg cggatgcggt
     1201 cctccatgtc cgcgccgtcg aggacatggc cgttctggtc ggtgtcgccg aacttgtcct
     1261 ggcgcgccat gttgcgcacg tggctctgcg cgatggcggt caaccgcgcg ttggcctcga
     1321 gcgcggcaag cgcgttctgc cggcggaaag cgttggtgcg cgcgatcacc tgctgctcga
     1381 tgccgctgac cgtgacgcca gctgccgccc gcggctcggc cgcttcgggc gcaggcgcgg
     1441 gtgcggcggg cttcgcggcc ggggcctggc gttgcggcgc gggtgcgcag gcggccagcg
     1501 cggcgagaat ggcgatgaca gtcggtgcgc gcagcatgat gacgacggcg ggttacaacg
     1561 cgaaccgggg aagcaaacgc cgtaccgcta gccgcctgtc ccgccgccgt tgacgtagat
     1621 cacctgcccg gtgacgtagc tcgcggactc cgaaaccaga aaagcgacca tcgccgcgat
     1681 ctcctccggc tgcccggcac gccccagcgg cacggtcttg agcaacgctt cggcgcgctc
     1741 ggcgccgagc ttctcgagcg tttcattcag catctcggtg cggatcggac cgggggccac
     1801 ggcgttgacg cgaatgcccg ccggcccctc ttccttggcg aggatgcggg tgaacgcctc
     1861 cagcgccgcc ttggacaccg cgtacgcacc gtagcccacg ggcatgcggt tggtgacgtt
     1921 ggaggagatg ttgacgatgt gcccgctgcc gcggctgcgc atgtgcggca gcaccgcccg
     1981 cgtcaggtgg aacggtccgt cgagattgat ccgcaggatc cgatcccatt cgccggccgg
     2041 catgtcgcgc agccaggcgc cgggcaagtg cgtgccggcg ttgttcacca gcacgtcgat
     2101 ccggcccatc gcctcgagcg cggcattcgc caggcgttgg cactcgtccg cgcgtcccgc
     2161 gtcggcgcgc acagcctgcg cctgaccgcc ctgcgccgcg gcggcggcga ccacctgctc
     2221 ggcctcgccg gcgcggctgc gatagctgaa cgtcacgcgc atcccggcct cgacgagcgt
     2281 caggacgatg gcgcgtccga tgccgcgcga gccaccggtc accagtgcgc agttcatgtc
     2341 ctcctcctca cgctttgccc ggatggggcg ctcgatgatg ccgcaagggc cgggcgggcg
     2401 caaacgcgct tgttgcggcc gcgggctcgg tgctataaga gggccgtccc cgcgacaacg
     2461 cgcccccgag gactcgagat gcccccgccc gtacccgatc agatgcaggt gcccgagaca
     2521 atgcaagtcg tggagatcac gcggccgggc ggaccggagg tcctgaagcc cgccacgcgc
     2581 ccggtgccgc aaccgcgcgc cggcgaagtg ctgatccagg tcgcggccgc gggcgtcaac
     2641 cgccccgact gcctgcagcg cgccggctcc tacgcgccgc ctccgggcgc ctccgatctg
     2701 cccggcctgg aggtcgcggg cacggtggta cgcgtgggcg aaggcgcgag cgagtggaat
     2761 gtcggcgacc gcgtctgcgc gctcacgcca ggcggcggct acgcgcagta ctgcctgacc
     2821 ccgggcgcac actgcctgcc gccggcgcgg gggctcgacc tgatccaggc ggcgtcgctg
     2881 ccagagacct tcttcaccgt ctggatcaac gtgttcgagc gcgcgcggct ggcgcccggc
     2941 gagacgctat tggtgcaggg cggcgccagc ggcatcggcg tcgccgccat ccagatggcg
     3001 cgggctttcg ggcaccgcgt gttcgccacc gccggcagcg cggaaaagtg cgcggcctgc
     3061 cagcgactgg gagccgaacg cgccatcaac taccgcacgc aggacttcgt cgaagcggtg
     3121 cagcaggcga ccggcggcaa gggtgtggac gtgatcctgg acatggtggg cggggactac
     3181 gtgccgcgcg agctgaaatg cctcgccaac gacgggcgcc tgtcgatcat cgccttcctc
     3241 ggcggcacca aggcgacgct ggacctgagc gacatcctgt tccgcaggct caccatcacc
     3301 ggctcgacgc tgcggccgcg cagcgtggag tacaaggccg gggtggcgca ggcgctgcgc
     3361 cagcgggtgt ggccgctcat cgaagccggc gccatccggc cggtgatcta ccgcaccttt
     3421 ccgctggcgc aggccgcgca agcgcacgcg ctcatggagt ccggcgcgca cgtcggcaag
     3481 atcgtgctgg aggtgatgcg cgagggctga ggcgcgcgac gtcaggccgc cagccgcacg
     3541 gcctggtcgg cgcgcgcagg cggattgcgg cgcagcagcc cgcgtgcttc gccgagctgc
     3601 gccacgcgcc gtgcgtgcga cacgttcttc tgcacagcgt cgaggtatag gttgcgtccg
     3661 ccgaaatgcg tgcgcgcgta gtcgacggcg ccgccgagca gttcacccag gcgccgctcg
     3721 gcgctgaggt agaaagccgg cgtcttgttc agcaggtcct cggccgacga gaactgcgcc
     3781 ccgggtgcag actcggcgcc gccgcgcgat gccagcccgc caacgacgaa ctccgggtac
     3841 aggcggtcgc ggcacttctc cagatagcag cgatcggaca tctgcgcgat gatgtccgcc
     3901 gtgcccagca tgttgccgat ccggcggaac agcgggctcg gcacccggat cgcgctcacc
     3961 ggcatctcgt agccggtgaa gtgaatcagc tcggcgccga ccgcggccag atcctccatg
     4021 ccgatgtcgg ccaggtagcg atgcaggaag cggccgccgc ggctgacatg acgcagcgtg
     4081 tactcggccc cgttctggtg gcgggtgtcg ttgcggcgcc gcagatagcc gatgtcgtgg
     4141 aacagcgcgg cgacgatgcc gaacacgaac aggcgctcgc cgatcggctc cgcgcgcgaa
     4201 gccgagcgct gatagccgtc catcaggcgc gccatcgcca ggctcacgtc cagcgtgtgc
     4261 tgcagatcgt ggtaggcggt gtcgcaggcg cggtaggccg ggtgggcgcc gcgatacatg
     4321 cgcgtgcagt cgtcgaaggc gcgcgacagg gtggacgtct gcgcgcgtgg gtagaggtcc
     4381 gcatgcaggc gtcgcacctc gccttcgacc gacgcggggt cggtggtgtc gatgcgattc
     4441 gacacgtcgt attcgcttct gcgggggtcc atgttgttct ccaaaccgac gatccgcgct
     4501 tgcacgggca tctcagccaa tcttcaatcc ttttgacaat ttcgaaccag ccgggcccgc
     4561 gaacgatcta ccgtcgtttg tcgacgacat ggactttagc atggaatcaa cgaccgggga
     4621 aggcagatgc ggggcagtgg ccagagccgg cggcagacgg gcctgcgaaa gatttgaatc
     4681 acctgccggg cgccgggccc ggcagggagg gcctagcgct gcgcgacggc ccggggcgat
     4741 tcgctgccgg cgtgcgcgcc cggcggggag gcgaggtccg gccgctgcgc ggccacggcg
     4801 ggcagatagc gggacagctc gcacagcgcc tcgtgataca ccgcgcgctt gaactcgacg
     4861 acgctgtcga gcggcaccca gtactggctc cagcgccagg cgtcgaactc cgggcgctcg
     4921 ctgcgccgca ggcagacgtc gcagtcgcgc ccgatcaagc gcagcagata ccagatctgc
     4981 ttctggccgc ggtagttcac gcgcgcatcg cgccgcaccc aagtcgccgg gacgtcgtag
     5041 cgcagccagt ggcgcgtgcg ccccacgacc ttgacgtgct cgggcaacag cccgacttcc
     5101 tccttcaact cccggtacat ggcgagctgc ggcgtttcgc cgggcttgat gccgccctgc
     5161 ggaaactgcc acgcgtgctc cttgacccgc ttgccccaga acacctcgtt gcgggcattg
     5221 accaggatga tgccgacgtt cgggcgatac ccgtcacgat cgatcacgac gatcacccca
     5281 agaaacctgt tcttgtccga attctttcat aaaccggccg gcctgggcaa tcgcgccggc
     5341 ggccgccgcc gcggcgcaag cgcagcccgg gcgggatcag atcagcagcc ggcggctggt
     5401 gtgcgggttg agccgccgca tgacgccgac gaaatcgccg aactcgatgc cgtaccgggc
     5461 ctgcagggcc gaggccgcgg tgcgcagctc gtcccagcgc gtggcgagcg gcgtgcgcct
     5521 gaaggcgagc accgcgacgt tgccgcgctg cgccgccggc acgcagcaga tgcgcccctc
     5581 gaaagcggcc tcgatgcgct gcagggtctc gtcgaagcgc cggtcgctgc cccacaggtt
     5641 gacgaccaga atgccgtcgg cggtgaggca gcgcgcggtg ctggcgtaga actcgcgggt
     5701 cgccagctcg gcggcgtggg agcggccgtc gtagccgtcc accagcacca ggtcgcaaca
     5761 gccggggtgc tgcgccgccc aggccgcgcc gtcggcctcg agcacgcgca accgggcatc
     5821 gtccccaggc aggtgaaagt actggcgcgc gcaggcgatc acgcgcgggt tgagctccag
     5881 caccagttgc tgggcgcgcg tcaggtgacg atggatccac ttggcgagcg agccgccgcc
     5941 caggccgatg ttgacgatac ggtgcgggtt cggcgcgaac agcaggaacg ccagcatgct
     6001 gcgggtgtag gacagcacca gctcgtgcgg atcggacagg cgcatcgcgc tctgcacggt
     6061 atccgtgccc aggtgcaggt agcgcacgcc gtcctgctcg ctgatctgca cctgcgcgtc
     6121 gccgccggcg ctgcgccggc gcaggcgcga cagcaacccg attcgcattc ccatctgcgt
     6181 gtcgctatct cgttgtctta tggctggttg tcttgcgggc cgactgtaga atagcggttt
     6241 tcccgggagt cccgatgcgc gtcagcgcct tctttctgtc caccctcaag gaagccccgg
     6301 ccgaggccga gttgccgagc cacaagctga tgctgcgcgc cgggctgatc cgcaagctgg
     6361 gcagtgggct gtacacgtgg atgccgctgg ggctgcgcgt gctgcgcggg gtggagcgca
     6421 tcgtgcgcga ggagatggat cgcgccggtg cggtggagct gttcatgccc gcggtgcagc
     6481 cggccgagct gtggcaggag tccggccgct gggacaagtt cgggccgcag atgctcaaga
     6541 tcaaggaccg gcacgagcgc gatttctgct tcggtcccac gcacgaggag gtgatcaccg
     6601 acatcgcccg ccgcgagatc cgcagctacc gccagctgcc ggtgaacttc taccagatcc
     6661 agaccaagtt ccgcgacgag atacggccgc gtttcggggt gatgcgggcg cgcgagttcg
     6721 tcatgaaaga cgcctactcc ttccacctgg atcacgccga cctggagcgc gaataccgca
     6781 acatgcacgc cgcctactcg cgcatcttcg agcgcctggg cctcaagttc cgcgcggtgg
     6841 ctgccgatcc cggagccatc ggcggcaccg gctcgcacga gttccacgtg ctggccgact
     6901 cgggcgagga cgccatcgcc ttcagcccgg agtcggatta cgccgccaac ctggagctgg
     6961 ccgaagcgct gccgccgcgc accccgcggc cggccccggc acagaaaatg cgcaagctag
     7021 ccacacccgg gcagcacacc atcgagcagg tgagcgcttt cctgaaagtg cccgccgagc
     7081 gcatcgtcaa aacgctgatg gtggtgaccg gggaccagca ggagtacctg ctgctgctgc
     7141 gcggggatca catgctgaac gaagtgaagg ccggcaagaa gttcctgctc aacgacggct
     7201 tccgcttcgc ctcggagggc gaggtgcgcg cgcgcctgcg cgccgcgccg gggtcgatcg
     7261 ggccggtggg cgaccaccag ctgccggtga tcgccgaccg cgcgctgcag gcgatgagcg
     7321 acttcgtctg cggcgccaac gaggatgacc accacctgct gggggtcaac ttcgggcgcg
     7381 atctgccaga gccggaggag tacatcgacc tgcgcaacgt ggtggaaggc gatccgagcc
     7441 cggacggcaa ggggcgcctc agcatctgcc gcggcatcga ggtcggccac atcttccagc
     7501 tgcgcaccaa gtactcggag gcgatgagcg ccaccgtgct ggacgaggcc ggccagccgc
     7561 gacccatgga gatgggctgc tacggcatcg gcgtgtcgcg catcgtggcc gccgccatcg
     7621 agcagaacca cgacgagcgc ggcatccttt ggccctcccc gatggcgccg ttccggatcg
     7681 cgatcgcgcc ggtcggctac ggcaagagcg caccggtgaa ggcgctcgcc gatcgcttgc
     7741 acgacgagct caccgccgcc ggcgtggagg cgctgctcga cgatcgtgac gagcgcccgg
     7801 gcgtgatgtt cgccgacctc gagctgatcg gcatccccca ccgcatcacc atcggcgagc
     7861 gcggcctgaa gcagggccag gtggagtacg tgtcgcggcg cgaaggcaca ccccggatga
     7921 tcgcggcgga tcaggcgctg gagttcgcca agacgctggg ctagaacccc aagaccgcag
     7981 gcgcagagga caaccgacaa aaggctcccg tgaccagctc caccggtatt tcgaaggttt
     8041 tctccgcgcc tctgtatctc tgcggtgcaa ggggtctctg cggtgcagtg agcctctgcg
     8101 gtgcagtggt gctcgcgctg tggcacgcgc cggcgttcgc gggcgcgcag cgctacgagc
     8161 cgctggcgac gagcgtgcgc gcggcgctgc actcggcggt ggccgaccgg gccagcccgc
     8221 gcctggtgtt cgcgaccccc gacgagggtt accagtggct ggacgagatg tcgatccgcc
     8281 tggtgcgccg cgtgcccgac cagcaaaccc gcctcgatct gctcaccacg gtgcagtacg
     8341 aagccaagcg cgccggcctc gacccgcaac tggtgctggg gctgatcgag gtggaaagcg
     8401 ggttccgcaa gtacgccgtg tcgcgcgcag gcgcccgcgg ctacatgcag gtgatgccgt
     8461 tctggttgaa tctgatcggc acgggcgacc acaacctgtt caacctgcgc accaacctgc
     8521 gctacggctg cgtcatcctg cgccactatc tcgatgtcga gcgcggcgac atgacccggg
     8581 cgctggcccg ctacaacggc agcctgggca agaagagcaa ttaccccgga aaggtgctgc
     8641 gcgcctggga gacgcgctgg ggctggccgg gcacgggcgt gcgccagatc tcggggaagc
     8701 gcccggcgat ccgctacatg ggcgagccgg actagcccac cgggctaagc cgcccgcgcc
     8761 cgtttggtcg ccgccgcgcc gcggcccgcg ccacgctcct cgaacagcga ttccagcgtc
     8821 atgctcagat cctcctgcac gtccgcggcg aggcgcgcga ccgcggcctc gtagccctct
     8881 tcgccgacgc gcacctgcgc ctcggcgtcg aacacgaact cgcggtaaac ctcggccagg
     8941 cggatcttgc cgacgtcgcg cgccagcacc cagcgctcgc ccgccgccgg caccacccag
     9001 ccgagcttcg ccagccgctc gagcagcgct tcgcactcct cccacggggt gcgcagcgcc
     9061 gcgcgcaggt cgccgacggt aagcacggca ccccggttgt gcgcctcgta cagcaagcgc
     9121 agcacgcgca ccgcatcgag caggtggctg ccaggcaagt accgcagccg tacgccgccg
     9181 gtgcgcaggt acggcatcgc cgcggtcagt tccgcaccga tcaggacgat cagccaggac
     9241 acgtagatcc aggtgaggaa gatcgggaaa ctcgcgaaag cgccgtatac cagcttgtag
     9301 gtgggcacct gcttgatgta ggcgccgaac aggcccttca tcagctcgaa cagcaggccc
     9361 gcgaccgccc cgccgaccac cgcatcggtg gtttgcacgc gccggtaggg cacggtgcga
     9421 tacaggaacg cgaaagtcag gcaggtcaac gcgagcggca ccactttgag gatcaccgcc
     9481 tccgggccgc gcagtttgcc gatcacgccg gtggactggg tgatcagcca ggaggtgagc
     9541 gacaggctgg ccccgagcag caagggcccc accgtcagca cgccccagta gatcagcacg
     9601 cgcgcgacca ccggccgcgc caggcgcacg cgccagatgc ggttgaaggt gtgctcgatg
     9661 gtgagcatca tcatgatcgc cgccgcgccc agcatcgcca tgcccagcgc cgtgagacgc
     9721 ccggcgttct ccgcgaactg ctgcacgtac acgccggtca gcttgctggc tgcagccggc
     9781 accaggtgcg tcagcatgaa attgcgtacc accgaccaga atccgccgaa ggcgggaaag
     9841 gccgagaaca cggtgagcgc gatggtcact accggcacca gggcgagcag cgaggtgaac
     9901 gtcagggcgc ccgagacctg cagcaggcgg tcctcgcgca ggcgcaagtg cagccagtgc
     9961 aggaagtcgg cgaaggcgcg cagctgacgg cgcatgcgga atggcttggc caaatgacgg
    10021 ttctataata gccgcggggt cacccgcctc ccgaaagcaa gggccattcc tcgcgcccga
    10081 cgccagcggt ccccctcacc gatgcccgag atcctggtcc tgtattacag ccgcaccggc
    10141 gcggtgcgac agatggcgca catggtcgcg cgcggcatcg acgcggtgcc gggggcggcg
    10201 gcgcgcatcc gcaccgtgcc cgcggtgtcc gcggtgagcg aggcggtcga gccgccggtg
    10261 cccgacgccg gcgcgcccta cgcgcagctt gccgacctcg aggcgtgcgc cggcctggcg
    10321 ctcggcagcc ccacccgctt cggcaacatg gccgcgccgc tcaagtactt cctcgattcg
    10381 acctcgggcc tgtggctgcg cggcgcgctg gccggcaagc cggcggcgct gttcacctcg
    10441 accggcacgc tgcacggcgg ccaggaggcc accctgctgt cgatgatgct gccgctgttc
    10501 caccacggca tgctgctgct cgggctgccc tataccgagc ccgagctcac caccacgacg
    10561 agcgggggca cgccgtacgg cgccagccac gtggccggcg ccgccgccga ccgggcggtg
    10621 accgacgagg agcagcgcat gtgcttcgcg ctggggcggc ggctggcgac cgtggcggcg
    10681 aagctggcgc gctgagatgg acgcgatgcc ccgcgcccgt gccgcgcgca tgctggtgcg
    10741 cagccaccgc agcggcgtgc tgtccagcca gtcgctgaag ttcccgggtt acccctacgg
    10801 ctcggcgctg ccgcacgtca ccgatcatgg cggccggccg gtggtgctga tcagccacct
    10861 cgccgagcac acccagaacc tggaggtgga cgcacgcgcg agtttcatcg tctgcgcgag
    10921 cggcgcggat ctgcaggcgc acccgcgcgc gaccgtgctg ggcgcggcgc gcgcggtggt
    10981 cgacgcggca ccggtcgcac gccgttatct gcgctattac cccgatcacg cgcagcacct
    11041 ggagatcggc gggttccggt tcttcgcgct ggagccgttc caggtgcgct tcatccaggg
    11101 cttcggcggg ctgcattgga tcgcgggcga gcgctatctc gcgccggaag cgatgcgcga
    11161 ggcggaggag gatatcctcg cgcacatgaa ccgggatcac ggcgaggcgt tgcgcgcgta
    11221 ttgcaggcac gtgcacggcg tcgaggtgga gaccgccgag atggtcggct gcgactgcga
    11281 cggcttcgac gtgcgcgcgg gcgagcggct gctgcgcttc ga
//
LOCUS       DASPGE010000029         9206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1178869, whole genome shotgun sequence.
ACCESSION   DASPGE010000029 DASPGE010000000
VERSION     DASPGE010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9206
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178869"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VD791_00845"
     CDS             complement(<1..1013)
                     /locus_tag="VD791_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXV06550.1"
                     /translation="MYYAFFGLKQPPFRITPDTDFFFEGGNRGAVLEALIYAIMRGEG
                     IVKVTGEVGSGKTMLCRVLQARLPPTVETVYLANPSVSPEEILHAIAFELQLPLARDA
                     GRLEVMHCLNEFLLARHAEGRQVVVFVEESQSMPIATLEEIRLLSNLETRQHKLLQIV
                     LFGQPELDVNLRKREIRQLRERITHSFSLAPLDSEEVSDYLGFRLRAAGYHGPDLFSP
                     RVIAYMARATGGLTRRLNIVADKALLAAFAEGTHNVSYKHVRAAIADSEFSADAGGGG
                     WLRWPAWVGAAALLALAAVGAGWLQRGTPPELPAMPSAAAPEIASTGTAAPAPTPGAT
                     QAAV"
     gene            complement(1024..2475)
                     /locus_tag="VD791_00850"
     CDS             complement(1024..2475)
                     /locus_tag="VD791_00850"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV06551.1"
                     /translation="MDSATSNKPDLLVVDDDPLITDALDFVLAHDFNVYVAESRNQAK
                     SLLRQLDTPPQLALVDLGLPPTPHRPDEGFQLINELLAFSPDIKILVLSGQNDENNAR
                     HARALGAIEFVHKPCEPEQLKRLLFKARTLTAAERGAGGQEAGILGSGPAIAKLRQQI
                     RQYAGSPFPVLIEGESGSGKELVAASLHGQSPRAAKPYLALNCAAISPTLVEPTLFGY
                     AKGAFTGATNHRAGYFEDARDGTLFLDEIGELPLELQAKLLRVLENGAYQRVGETSTR
                     TSQARVLAATNRDLKQEVRTGRFRPDLYHRLSVFTIAVPPLRELGEDKRLLLQHFAEF
                     YARQGGAAPVVLDERAERIWMEYPFPGNVRELRNVVIRLTAKYAGEPVNSEQLLAELD
                     TQSLEPAGGPGAAALGADFRGSVEAAKQHLQSQRNFSLDETLQRCERSYVEAALALTS
                     GNLSQAAKLLGIHRTTLYSRMQNYAPGLTADKD"
     gene            complement(2619..3377)
                     /locus_tag="VD791_00855"
     CDS             complement(2619..3377)
                     /locus_tag="VD791_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV06552.1"
                     /translation="MTTGTDMNEPGKGTALRFDAVSKRYGTTVALQALSLAVARGEAF
                     GLAGMNGAGKTTLIKCLLDFCALDAGRIEVFGVSHRLTSARSRLAFLPERFIPPYYLT
                     GRDFLRYMLTLYGVTYAQAPVEETLRALDLEAAALTKPVRAFSKGMTQKLGLAACLLS
                     GRELLVLDEPTSGLDPRARALLKQRLRALRGGGGTVFFTSHALADVEEICDRMAVIHR
                     GELCFAGTPAQLQAEHGAATLEAAFLARIERAAA"
     gene            3540..4361
                     /locus_tag="VD791_00860"
     CDS             3540..4361
                     /locus_tag="VD791_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV06553.1"
                     /translation="MQSVVTQNLVEIEDVEFRYDVRPVLKGIHMRFPRGKVIAVMGLS
                     GCGKTTTLRLIAGQLRPARGSVRVAGQRVQDLDQGELYALRRRMGMLFQFGALFTDMS
                     VFDNVAFQLREHTGLPEAVVRDLVMMKLNAVGLRGAHALMPAELSGGMARRVALARAI
                     ALDPKLIMYDEPFAGLDPISLGVIGNLIRRLNDALGATSIVVTHDVQESLKIVDYVYF
                     MSEGVVVAQGSADEIKAAREDYVRQFVHGEMDGPVPFHYAGPTYRRDLRLEPTHG"
     gene            4354..5148
                     /gene="mlaE"
                     /locus_tag="VD791_00865"
     CDS             4354..5148
                     /gene="mlaE"
                     /locus_tag="VD791_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HXV06554.1"
                     /translation="MDNLLVRALRHVGHLVIDGVWRLGYASRFFLLVLLRSGPSFRRF
                     PLTIREIYFAGVLSLIIILVSGLFVGMVLGLQGYDTLQRFGSEEAVGTLVALSLVREL
                     GPVVCALLFASRAGSAMTAEIGLMKATEQITALDMMAIDPIARIVGPRFWGGVLSMPL
                     LAALFSAMGVFGGYLITVVLLGVDAGAFWSQMQASVDLKEDVLNGVIKSIVFGVAVTA
                     IALFEGYDAPPTAEGVSGATTRTVVTSSLAVLALDFVLTAIMFTGA"
     gene            5152..5631
                     /gene="mlaD"
                     /locus_tag="VD791_00870"
     CDS             5152..5631
                     /gene="mlaD"
                     /locus_tag="VD791_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HXV06555.1"
                     /translation="MDRRVLDLWVGIFVAAGIAALFVLALQVSNASTFNTGESYRVIA
                     EFDNIGGLRVRAPVKSAGVLVGRVEGIGFDNDKFVARVVLRLDRRYTFPKDTIASILT
                     SGLLGEQYIGLDGGGDPENLADGERLKLTQSAVVLERLIGQFLYGRAAEGGTAGESK"
     gene            5732..6388
                     /locus_tag="VD791_00875"
     CDS             5732..6388
                     /locus_tag="VD791_00875"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV06556.1"
                     /translation="MHRILCSFVATLLLAVAGLLPAPEALAQKAPDELVKSVAEDVLA
                     IVRQDRALREGDNSRMAQLIEEKIVPHFDFERMTRLAVGRSWRQATPEQRQQLIDEFR
                     RLLVRSYSAAYSTYNHIVVEVKPLKLQPGEEDVQVKTLIKLPGGAPPVSVDYSMVNNS
                     PDWKVYDVVVDGISLVTTYRTTFAEEVNKGGIEGLLKSLAALNAAKTAQRPVGATRRQ
                     "
     gene            6405..6722
                     /locus_tag="VD791_00880"
     CDS             6405..6722
                     /locus_tag="VD791_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXV06557.1"
                     /translation="MIVRDGACFRVEGPVTMANVEALIEEGRAAFADGAVQVDLAGVT
                     EVDSTAVSLLLQWLRDAAARGRRLTFRNLNHNIHSLAVLYGVAELIPIDDASEPRRTG
                     APG"
     gene            6761..7660
                     /locus_tag="VD791_00885"
     CDS             6761..7660
                     /locus_tag="VD791_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV06558.1"
                     /translation="MAPAIEIRDARKCYGTLRALDHVSLEIAQGEFFGLLGPNGAGKT
                     TLISLLAGLARADSGSLKVMGHDVVRDFRAARRALGVVPQELVFDPFFSVRETLRIQS
                     GYFGLRRNDAWIDEILERLDLTHRADSNMRTLSGGMKRRVLVGQALVHRPAVIILDEP
                     TAGVDVELRQGLWKFIRELNAAGHTIVLTTHYLEEAEALCGRIAMLKQGRVVALDTTR
                     NLLQHFSGVRIRIRLLPDVLPPDLGPYHVAREGESEILQLPDYGAIERVLAALRAAEV
                     QAQELEVLQADLEQVFVQMMRKS"
     gene            7660..8415
                     /locus_tag="VD791_00890"
     CDS             7660..8415
                     /locus_tag="VD791_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV06559.1"
                     /translation="MSGFATLLYKELLRFWKVTFQTVGAPILTALLYLLIFSHVLESR
                     VQVYPGVSYSAFLAPGLAMMSLLQNAFANSSSSLIQSKITGNIVFVLLTPLTPLQMFL
                     AYVLAAIARGLLVGIGVLLSTLLLADVPLLHPLWALTFAVLGGAILGALGIIAGIWAD
                     KFDQLAAFQNFLIVPLTFLAGVFYSVHSLPDFWQRASHLNPFFYMVDGFRYGFFGAGD
                     VAPLTSLAVVAGCLAGLSALTLRLLHIGYKLRG"
     gene            8459..8710
                     /locus_tag="VD791_00895"
     CDS             8459..8710
                     /locus_tag="VD791_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HXV06560.1"
                     /translation="MVLPDDVKTYIQQGLDCEHVEVVGDGQHFEALIVSPAFRGKSRV
                     QQYQLVYRVLGERMREEIHALSMQTFTPEDWAGRQARPG"
     gene            8732..>9206
                     /locus_tag="VD791_00900"
     CDS             8732..>9206
                     /locus_tag="VD791_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HXV06561.1"
                     /translation="MDKLLIQGGVALSGEVRVSGAKNAALPILCASILTAGTLRVRNV
                     PQLRDVTTTLALLRQMGVEVSLDEKLGVGLVAAGVHHRVAPYELVKTMRASILVLGPL
                     LARFGEARVSLPGGCAIGTRPVDLFIEGLTALGANIEIDGGYVNATAPEGGLTGGR"
BASE COUNT         1347 a         3078 c         3269 g         1512 t
ORIGIN      
        1 accgccgcct gcgtcgcgcc cggcgtcgga gcaggcgctg cggtcccggt cgacgcgatc
       61 tcaggcgccg ccgccgaggg cattgccggg agctcgggcg gcgtaccacg ctgaagccac
      121 cccgcgccaa cggccgcgag cgcgagcagc gccgccgccc ccacccacgc cggccagcgc
      181 agccagccgc ccccgccggc gtcggcgctg aactcgctgt ccgcgatggc agcgcgcacg
      241 tgtttgtacg agacgttgtg cgtgccctcg gcgaacgccg ccagcagagc cttgtcggcg
      301 acgatgttca ggcgccgcgt caagccgccg gtggcgcgcg ccatgtaggc gatcacgcgc
      361 ggcgagaaca ggtcgggccc gtgatagccg gccgcgcgca ggcggaagcc gagatagtcg
      421 ctcacctcct cggagtcgag cggcgccagc gagaagctgt gcgtgatgcg ctcgcgcagc
      481 tggcggatct cgcgcttgcg caggttgacg tcgagctcgg gttgcccgaa cagcacgatc
      541 tgcagcagct tgtgctggcg cgtctcgagg ttcgacagca ggcggatctc ctccagggtg
      601 gcgatcggca tgctctgcga ttcctcgacg aacaccacca cctgcctgcc ctcggcgtgc
      661 cgggcgagca ggaactcgtt gaggcagtgc atcacctcca ggcggccggc gtcgcgcgcg
      721 agcggcaact gcagctcgaa ggcgatggca tgcaggatct cctcgggcga gacgctgggg
      781 ttggccaggt acaccgtctc caccgtgggc ggcagccgcg cctgcagcac gcggcacagc
      841 atggtcttgc cgctgcccac ttcgccggtg accttgacga tgccctcgcc gcgcatgatt
      901 gcgtagatca gcgcctccag caccgcgccg cgattgccgc cctcgaagaa gaaatcggtg
      961 tccggcgtga tgcggaaggg cggctgcttg aggccgaaga aggcgtagta cataggtctg
     1021 cgatcaatcc ttgtcggcgg tgagcccggg cgcgtagttc tgcatccggc tgtacagagt
     1081 ggtgcggtgg atgccgagca gcttggcggc ctggctcaga ttgccgctgg tcagcgccag
     1141 cgccgcttcc acgtagctgc gctcgcagcg ctgcagcgtc tcgtccaggc tgaagttgcg
     1201 ctgcgactgc aggtgctgct tggcggcctc gaccgagccg cggaaatcgg cgccgagcgc
     1261 cgccgcgccg gggccgccgg ccggctcgag gctctgcgtg tccagctcgg ccagcagctg
     1321 ctcgctgttc accggctcgc cggcgtactt cgcggtgagg cggatcacga cattgcgcag
     1381 ctcgcgcacg ttgcccggga aagggtactc catccagatg cgctcggcgc gttcgtccag
     1441 gacgaccggc gccgcgccgc cctggcgcgc gtagaactcg gcgaagtgct gcagcagcag
     1501 ccgcttgtcc tcgcccagct cgcgcaacgg cggcacggcg atggtgaaca cgctcaggcg
     1561 gtgatagaga tcgggacgga agcggccggt gcgcacctcc tgcttcaggt cgcggttggt
     1621 ggcggccagc acgcgcgcct ggctggtgcg cgtgctggtc tcgcccacgc gctggtaggc
     1681 gccgttttcc agcacgcgca ggagcttggc ctgcagctcg agcggcagct cgccgatctc
     1741 gtccaggaac agggtgccgt cgcgggcgtc ctcgaagtag ccggcgcggt ggttggtggc
     1801 gccggtgaag gcgcccttgg catagccgaa cagtgtcggc tccaccaagg tgggcgagat
     1861 ggccgcgcag ttgagcgcca ggtagggctt ggcagcgcgc gggctctggc cgtgcagcga
     1921 ggcggccacc agctccttgc cgctgcccga ttcgccttcg atcaacaccg ggaagggcga
     1981 gccggcgtac tggcggatct gctgccgcag cttggcgatc gccggcccgc tgccgaggat
     2041 gcccgcttcc tggccgccgg cgcctcgctc ggcggcggtc agggtgcgcg ccttgaacaa
     2101 cagccgcttg agctgctccg gctcgcacgg cttgtgcacg aactcgatcg cgcccagtgc
     2161 ccgggcgtga cgcgcattgt tctcgtcgtt ctgcccggaa aggaccagga tcttgatgtc
     2221 gggcgagaag gccagcagct cgttgatcag ctggaagcct tcgtcggggc ggtgcggggt
     2281 ggggggcaac cccagatcga cgagcgcgag ctgcggaggc gtatccagct ggcgcagcag
     2341 actcttcgcc tgattgcgcg actcggccac gtagacgttg aagtcgtgcg ccagcacgaa
     2401 atcgagcgcg tccgtgatca gcggatcgtc gtcgacgacg agcaggtcag gcttgttgga
     2461 ggtggcgctg tccattcgca tgcaccgatg cgggcggccc gatcaaatat tgtgccagat
     2521 ggcagcggcc cgccggcggc gcccgcgaac aaacgccggc ggacgctcgc gcgtcgacct
     2581 gtcgccggga cgccgggccg gcgcatcgag tacccgactc aggctgccgc gcgctcgatg
     2641 cgggccagga aggctgcctc gagggtggcc gcgccgtgct cggcttgcag ctgcgccggg
     2701 gtgccggcga agcacagctc gccgcggtgg atcaccgcca tgcggtcgca gatctcctcc
     2761 acgtcggcca gcgcgtggga ggtgaagaac acggtgccgc cgcccccgcg cagcgcccgc
     2821 aggcgctgct tgagcaacgc gcgcgccctc gggtcgagcc cgctggtcgg ttcgtcgagc
     2881 acaagcagct cgcggccgct cagcaggcag gcggcgagcc ccagcttctg ggtcatgccc
     2941 ttggagaagg cgcgcaccgg cttggtgagc gcggccgcct ccaggtcgag cgcgcgcagc
     3001 gtctcctcga ccggcgcctg ggcgtacgtc acgccgtaca gcgtcagcat gtagcgcagg
     3061 aagtcgcggc cggtcaggta gtagggcggg atgaagcgct ccgggaggaa ggcgagccgg
     3121 ctgcgggccg aggtgaggcg gtggctcacg ccgaacacct cgatgcggcc ggcgtccagc
     3181 gcgcagaagt cgagcaggca cttgatcagc gtggtcttgc ccgcgccgtt catgcccgcc
     3241 aggccgaagg cctcgccccg ggccacggcg aggctcagcg cctgcagcgc aacggtggtg
     3301 ccgtagcgct tgctgaccgc gtcgaagcgc agagcggtgc ccttgccggg ttcgttcatg
     3361 tcggtgccgg tcgtcatgcg tccaccgggc gcaacagtgc ccggaatgcc agccgctggc
     3421 aagcgcgcgt ccccccaggg gcctctcctt acacggggtt ctctgctaca acgggcttgt
     3481 ctgctgcgac cggcacccct gctacaatac gccgcgcttt gcaaccgccg atggagtctg
     3541 tgcagtctgt ggtcacgcag aacctggtcg agatcgaaga cgtcgagttc cgctacgacg
     3601 tgcgccccgt gctgaagggc atccacatgc gcttcccgcg cggcaaagtg atcgcggtca
     3661 tgggcctgtc cggctgcggc aagaccacca cgttgcgcct catcgccggc cagctgcggc
     3721 ccgcccgcgg cagcgtgcgc gtggccgggc agcgggtgca ggatttggac cagggtgagc
     3781 tgtacgcgct gcgccggcgc atgggcatgc tgttccagtt cggcgcgctg ttcaccgaca
     3841 tgtcggtgtt cgacaacgtg gcgtttcagc tgcgcgagca caccggtctg ccggaggccg
     3901 tggtgcgcga cctggtgatg atgaagctca acgcggtggg gttgcgcggc gcgcacgcgc
     3961 tgatgccggc cgagctctcc ggcggcatgg cgcggcgcgt ggcgctggcg cgcgccatcg
     4021 cgctggaccc gaagctgatc atgtacgacg agccgttcgc gggactggat ccgatctccc
     4081 tgggggtcat cggcaatctg atccggcgct tgaacgacgc gctgggcgcg acctcgatcg
     4141 tggtcaccca cgacgtgcag gagtcgctca agatcgtcga ctacgtgtac ttcatgtcgg
     4201 agggggtggt ggtggctcag ggcagcgcgg acgagatcaa ggccgcgcgc gaggactacg
     4261 tgcgccagtt cgtgcacggc gaaatggacg gtccggtgcc gttccattac gccggcccga
     4321 cttatcggcg cgacctgcgc ctggaaccca cgcatggata acctgctggt gcgcgcgctg
     4381 cgccacgtcg gacacctggt gatcgacggc gtgtggcgcc tgggctacgc cagccgcttc
     4441 ttcctcttgg tgctgctgcg ctcgggcccg agcttccggc gtttcccgct caccatccgc
     4501 gagatctact tcgcgggcgt gctctcgctc atcatcatcc tggtgtcggg gttgttcgtc
     4561 ggcatggtgc tgggcctgca gggctacgac acgctgcagc gcttcggctc ggaggaggcg
     4621 gtcggcaccc tggtggcgct gtccctggtg cgcgagctcg ggccggtggt gtgcgccctg
     4681 ctgttcgcca gccgtgccgg ctcggccatg accgccgaga tcggcctgat gaaggccacc
     4741 gagcagatca ccgcgctcga catgatggcg atcgacccga tcgcgcgcat cgtcgggccg
     4801 cgcttctggg gcggggtgct gtcgatgccg ctgctggccg cgctgttcag cgccatgggc
     4861 gtgttcggcg gctacctgat cacggtggtc ctgctggggg tggacgccgg ggcgttctgg
     4921 tcgcagatgc aggcctcggt cgacctcaag gaagacgtgc tgaacggcgt cataaagagt
     4981 atcgtgttcg gcgtggcggt caccgccatc gccctgttcg agggctacga cgcgccgccc
     5041 acggccgaag gcgtgtccgg cgcgaccacg cgcacggtag tgacgtcctc gctcgcggtg
     5101 ctggcgctgg atttcgtgct caccgcaatc atgttcacgg gtgcataacg catggatcgc
     5161 agagttctcg acctgtgggt cggtatcttc gtggcggcgg gcatcgccgc cctgttcgtg
     5221 ctcgcgctgc aggtgagcaa cgccagcacc ttcaacaccg gcgagagcta ccgcgtgatc
     5281 gcggagttcg acaacatcgg cggcttgcgg gtgcgcgccc cggtgaagag cgccggcgtg
     5341 ctggtcgggc gcgtggaggg catcggcttc gacaacgaca agttcgtggc gcgggtggtg
     5401 ctgcgcctgg accgccgcta caccttcccg aaggacacca tcgcctcgat cctcacctcc
     5461 ggcctgctgg gcgagcaata catcggcctg gacggcggcg gcgatcccga gaatctggcc
     5521 gacggcgaga ggctcaagct cacgcagtcg gcggtggtgc tggagcgcct gatcggccag
     5581 ttcctctacg gccgcgcagc ggagggtgga acggcgggcg aatcgaagtg accccgccgt
     5641 tgtccaaaca cggggcgttg tcccaccacc gggcgtggtc caaacatgcg gcgttgtcca
     5701 agcatccggc caaacgttca taggactcga aatgcaccgc atcctttgta gtttcgtcgc
     5761 aactttgctg ttggcggtcg ctggcctgct gccggcgccg gaggcgctgg cgcagaaggc
     5821 gcccgacgag ctggtcaaga gcgtggcgga ggacgtgctc gccatcgtgc gtcaggaccg
     5881 cgcgctgcgc gagggcgata actcgcgcat ggcgcagctg atcgaggaga agatcgtccc
     5941 gcacttcgac ttcgagcgca tgacccgcct ggcggtgggc cgctcttggc gccaggcgac
     6001 gccggaacag cgccagcagc tgatcgacga gttccgccgc ctgctggtgc gttcctacag
     6061 cgccgcctac agcacctaca accacatcgt ggtggaggtc aagccgctca agctgcagcc
     6121 cggggaggag gacgtgcagg tcaagacgct gatcaagctc cccgggggtg ccccgccggt
     6181 gagcgtcgac tacagcatgg tcaataacag ccccgactgg aaggtctacg acgtcgtcgt
     6241 ggacggcatc agcctggtga ccacatatcg gaccaccttc gccgaggagg tcaacaaggg
     6301 cggcatcgag gggctgctca agagtctggc ggcgctcaac gccgcgaaaa cggcacagcg
     6361 cccggttggg gcgacccggc gtcaataggc cgggagctcg acagatgatc gtacgcgacg
     6421 gtgcctgctt ccgggtggag ggcccggtca ccatggccaa cgtcgaagcg ttgatcgagg
     6481 agggccgcgc cgcgtttgcc gacggcgcgg tgcaggtgga cctcgccggg gtcaccgagg
     6541 tggactccac cgccgtcagt ctgctgctgc agtggttgcg cgacgcggcc gcacgcggcc
     6601 gccgcctcac cttccgcaat ctgaaccaca acatccacag cctcgccgtg ctgtacggcg
     6661 tggccgagct gatccccatc gacgacgcca gcgaaccaag aagaaccggc gcgcccggct
     6721 gaggtgtacg cggacgcccc tgggcatgcg cggcagcgcc atggcaccgg ccattgaaat
     6781 ccgggatgcg cgcaagtgct acggcacgct gcgggcactc gaccacgtca gcctggagat
     6841 cgcgcagggc gagttcttcg gcctgctcgg gccgaacggc gccggcaaga ccacgctgat
     6901 cagcctgctg gcgggcctgg cgcgggccga ctccggcagc ctgaaggtga tgggccacga
     6961 cgtggtgcgc gactttcgcg ccgcccgccg ggcgctgggc gtggtgccgc aggagctggt
     7021 gttcgacccc ttcttcagcg tgcgcgagac cctgcgcatc cagtccggct acttcgggct
     7081 gcggcgcaac gacgcctgga tcgacgagat cctcgagcgc ctggacctga cccacagggc
     7141 ggacagcaac atgcgcaccc tgtccggagg catgaagcgc cgcgtgctgg tggggcaggc
     7201 gctggtgcat cgcccggcgg tgatcatcct ggacgagccc accgcagggg tcgacgtgga
     7261 gctgcgccag gggctgtgga agttcatccg cgagctcaac gccgccggcc ataccatcgt
     7321 gctgaccacg cactacctgg aggaggccga ggcgctgtgc ggccgcatcg ccatgctcaa
     7381 gcaggggcgg gtggtggcgc tggacacgac gcgcaacctg ctgcagcact tctccggcgt
     7441 cagaattcgc atccggctgc tgcctgacgt gctgccgccg gacctggggc cctaccatgt
     7501 cgcgcgcgag ggcgagagcg agatcctgca actgcccgac tacggcgcca tcgagcgggt
     7561 gctggcggcg ctgcgcgcgg cggaggtgca ggcgcaggag ctcgaggtgc tgcaggccga
     7621 cctggagcag gtgttcgtac agatgatgcg caagtcctga tgagcggctt tgccaccctg
     7681 ctgtacaagg agctgttgcg cttctggaag gtgaccttcc agaccgtcgg cgcgccgatc
     7741 ctcaccgcgc tgctctatct gctgatcttc tcgcacgtgc tggaatcgcg cgtgcaggtc
     7801 tacccaggcg tgagctacag cgccttcctg gcgcccgggc tggcgatgat gtcgttgctg
     7861 cagaacgctt tcgccaatag ctcctccagc ctgatccaat cgaagatcac cggcaacatc
     7921 gtgttcgtgc tgctcacgcc gctcacgccc ctgcagatgt tcctggccta cgtgctggcg
     7981 gcaattgcgc gcggcctcct ggtgggtatc ggcgtgctcc tgtccaccct gctgctggcc
     8041 gacgtccccc tgctgcatcc gctgtgggcg ctgaccttcg ccgtgctggg cggcgcgatc
     8101 ctcggcgcgc tcggcatcat cgccggcatc tgggccgaca agttcgacca gctcgccgcg
     8161 ttccagaact tcctgatcgt gccgctcacc tttctcgccg gcgtgttcta ttcggtgcac
     8221 tcgctgcccg acttctggca gcgcgcgtcg cacctgaatc cgttcttcta catggtggac
     8281 gggttccgct acggcttctt cggcgccggc gatgtggcgc cgctgacgag cctggcggtg
     8341 gtcgcgggtt gcctggcggg gctgtccgcg ctgactctgc ggctgctgca catcggctac
     8401 aagctgcgcg gctgacgggc gctacaatag cgatccgagt cattgcaggg gaatcgccgt
     8461 ggtcctgccg gacgacgtca agacatacat tcagcagggg ctcgactgcg agcacgtgga
     8521 agtggtcggc gacgggcagc atttcgaggc gctgatcgtc agcccggcgt tccgcggcaa
     8581 gagccgagtg cagcagtatc agctcgtcta ccgggttttg ggcgagcgca tgcgcgagga
     8641 gatccacgcg ctgtcgatgc agaccttcac gcccgaggac tgggccgggc gccaggcccg
     8701 gcccggataa tttccggccc gctcgattcc catggacaag ctgctgatcc agggcggggt
     8761 cgccctgtcc ggcgaggtgc gggtgtcggg tgcgaagaac gccgcgctgc cgatcctgtg
     8821 cgcgtcgatc ctcaccgccg gcaccctgcg ggtgcgcaac gttccgcagt tgcgcgatgt
     8881 caccaccacg cttgcgctgc tgcggcagat gggtgtggag gtgtcgctcg acgagaagct
     8941 cggcgtcggg ctggtcgccg ccggagtcca ccaccgggtc gcgccctacg agctggtcaa
     9001 gaccatgcgc gcctccatcc tggtgctggg cccgctgctg gcgcgcttcg gcgaggcgcg
     9061 cgtctcgctg ccgggcggct gcgccatcgg cacgcggccg gtcgatctct ttatcgaggg
     9121 cctgacggcg ctcggcgcca acatcgagat cgacggcggc tacgtcaacg ccacggcgcc
     9181 ggaaggcggg ctgaccggcg ggcgct
//
LOCUS       DASPGE010000030         3471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1530687, whole genome shotgun sequence.
ACCESSION   DASPGE010000030 DASPGE010000000
VERSION     DASPGE010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530687"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VD791_00905"
     CDS             <1..822
                     /locus_tag="VD791_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV06562.1"
                     /translation="GLGRAMALAFADEGMDLALADVDEKGLKETQSAVEAKGVKASWM
                     KVDVSKEAEVQKFCDLLPAVHVVCNNAGVSPLGAVWENSAADWQWILGVNLWGVIHGV
                     RAFAPRLIAQDEGHIVNTASVAGLISPPGMGAYNVTKHAVVALSESLYHDLRERNSRV
                     GVSVLCPAYVPTGIVDSERNRPALLRNPPRTKTSEELAREEQMRHAVLSGRISAERVA
                     DKVLEAVREDRFYILTHAKIKSAIQTRMEDILLERRPTDTSKPGDTAKPAGKPGG"
     gene            complement(831..2051)
                     /locus_tag="VD791_00910"
     CDS             complement(831..2051)
                     /locus_tag="VD791_00910"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXV06563.1"
                     /translation="MTGVSRRAQELGTENAFVVLAEVNELVRAGRDVISFCIGQPDFH
                     TPVNIQDAAIKAIREGKHGYTPSAGIVELRQAVAREISATRGIAVDANDVVIAAGAKP
                     FIGYTVLSVTDYGAGDEVIYPNPGFPIYESQIKANGAVPVPLHLRESRAFNFDPQELA
                     DRITPRTKLLILNSPHNPTGGVIPRQDMEAIAEILRKHEQVWVFADEIYGRLVYDGEF
                     ASIASLPGMQERTIVSDGCSKTWAMTGWRLGFAVNRKLAPFFTTWITNTESCASQISQ
                     WAAVEALTGPQDDARRMRDIFFERRNLIVELLNRVPGVTCQSPGGAFYVWPNVTQACA
                     MVGAKDSEELRARLLNEAGVAVLADIHFGPRVPNEGQHIRFSYAASNDAIRKGVARMA
                     EFIEKNRAKRSAAA"
     gene            2168..3166
                     /locus_tag="VD791_00915"
     CDS             2168..3166
                     /locus_tag="VD791_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HXV06564.1"
                     /translation="MATNMQVLLASRPTGWVEESNFRIVESPIPRPGPGEVLVKVHWL
                     SLDPYMRGRMNAGKSYARAVEIGEVMTGGTVAEVIESTHRKFKPGDHVVGSLGWQQYA
                     VSDGEGLTKVDPKLVPLSYYLGAVGMPGATAWIGLLEHCQPRAGETVVVSAASGAVGS
                     VVGQLAKMQGCRAVGIAGGSAKCDYVVRELRFDACVDHKGGKLYDDLKAACPEGIDCY
                     FENVGGEVMDTVFRQLNPFSRVALCGMIADYNLTEPYGVKMLRALLMNRVKLQGFIVS
                     DRMDLYQRALQRLGKAVAEGKIKYRETVAQGLENAPKAFIGMLKGANLGKQLVKVA"
     gene            complement(3171..>3471)
                     /locus_tag="VD791_00920"
     CDS             complement(3171..>3471)
                     /locus_tag="VD791_00920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004544446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV06565.1"
                     /translation="AWLSFSSFYGRPAYDKTAELSVYVDQRARGQGLGSYLLRAALAQ
                     APSIGVDRLLGFIVGHNAPSLRLFAKFGFERWGFMPGVARLDGVERDLVVVGRRV"
BASE COUNT          588 a         1137 c         1221 g          525 t
ORIGIN      
        1 ggcctgggcc gcgccatggc gctcgccttc gccgacgagg gcatggacct ggcgctcgcc
       61 gatgtcgatg aaaagggatt gaaagagacg cagtccgccg tggaagccaa aggcgtcaag
      121 gcatcctgga tgaaggtgga cgtctcgaag gaagcggagg tgcagaagtt ctgcgatctg
      181 ctgccggcgg tccacgtggt gtgcaacaac gccggcgtct cgccgctcgg cgcggtgtgg
      241 gagaacagcg ccgccgactg gcaatggatc ctcggcgtca acctgtgggg cgtgatccac
      301 ggcgtgcgcg ccttcgcgcc gcgcctgatc gcgcaggacg agggccacat cgtcaacacc
      361 gcctcggttg ccgggctgat ctcgccgccc gggatgggcg cctacaacgt caccaagcac
      421 gcggtggtcg cgctgtcgga atcgctgtac cacgacctgc gcgagcgcaa ctcgcgcgtc
      481 ggcgtctcgg tgctgtgccc ggcctacgtg cccaccggca tcgtcgactc cgagcgcaac
      541 cggcccgcgc tgctgcgcaa cccgccgcgc accaagacgt ccgaggagct ggcgcgcgag
      601 gagcagatgc gccacgcggt tctctccggc cgcatcagcg ccgagcgggt ggccgacaag
      661 gtgttagagg cggtgcgcga ggatcgcttc tacatcctga cgcacgcgaa gatcaaaagc
      721 gccatccaga cgcggatgga ggacatcctc ctggagcggc ggcctacgga cacgtcgaaa
      781 ccgggagaca ccgcgaagcc cgccgggaag cccggcggct gagcgccggg tcaggcggcc
      841 gcgctgcgct tcgccctgtt cttctcgatg aactcggcca tccgcgccac ccccttgcgg
      901 atggcgtcgt tcgaggccgc gtagctgaag cggatgtgct ggccctcgtt gggtacgcgc
      961 gggccgaagt ggatgtcggc gagcacggcc acaccggcct cgttgagcag gcgtgcgcgc
     1021 agctcctccg agtccttcgc acccaccatc gcgcaggcct gcgtgacgtt gggccacacg
     1081 tagaacgcgc cgccggggct ctggcaggtc acgcccggaa cgcggttcag cagctccacg
     1141 atcagattgc gccgctcgaa gaagatgtcg cgcatgcgcc gggcgtcgtc ctgcggtccg
     1201 gtcagcgcct cgaccgcggc ccactggctg atctgggaag cgcacgactc ggtgttggtg
     1261 atccaggtgg tgaagaacgg cgcgagtttg cggttcacgg cgaagcccag gcgccaaccg
     1321 gtcatggccc aggtcttgga gcagccgtcg gagacgatgg tgcgctcctg catgccgggc
     1381 agcgacgcga tcgacgcgaa ctcgccgtcg tacaccagcc gaccgtagat ctcgtcggcg
     1441 aacacccaca cctgctcgtg cttgcgcagg atctcggcga tcgcctccat gtcctggcgc
     1501 gggatcacgc cgccggtggg attgtgcggc gagttcagga tcagcagctt ggtgcggggc
     1561 gtgatcctgt cggccagctc ctgagggtcg aagttgaacg cgcgcgactc gcgcaggtgc
     1621 agcggcaccg gcaccgcgcc gttggccttg atctgcgatt cgtagatcgg aaagcccggg
     1681 ttgggataga tcacttcatc gcccgcgccg tagtcggtga ccgacagcac ggtgtagccg
     1741 atgaacggct tggccccggc cgcgatcacc acgtcgttgg cgtcgaccgc gatgccgcgc
     1801 gtggccgaga tctcgcgcgc caccgcctgg cgcagctcga cgatgcccgc cgagggcgtg
     1861 tagccgtgct tgccctcgcg aatcgccttg atcgccgcgt cctggatgtt gaccggcgta
     1921 tggaaatctg gctgcccgat gcagaacgag atgacgtcgc gcccggcgcg gaccagctcg
     1981 ttcacctcgg ccagcaccac gaaagcgttc tcggtgccga gctcctgcgc gcggcggctc
     2041 accccggtca tggaagttcc ctctgccgat gcaaagagcg taggttatca tgcggcgact
     2101 ggccgcgaac tcttgactgc gaaagcctga ctgcgaaacc cctggttgcg aaacgaggat
     2161 ctcccacatg gcgacgaaca tgcaggtgct gctggcgagc cggccgacgg gctgggtcga
     2221 ggaatcgaac ttccgcatcg tggagagccc gatcccgcga ccgggccccg gcgaagtgct
     2281 ggtcaaggtg cattggctct cgctcgatcc ctacatgcgc gggcgcatga acgccggcaa
     2341 gtcctacgcc agggcagtgg agatcggcga ggtgatgacc ggcggcaccg tggccgaggt
     2401 gatcgaatcc actcaccgca agttcaagcc gggcgaccac gtggtcggca gcctgggatg
     2461 gcagcagtac gcggtgagcg acggcgaagg gctcacgaag gtcgatccca agctggtgcc
     2521 gctgtcgtac tacctgggtg cggtgggcat gccgggcgcg accgcctgga tcggcctgct
     2581 cgagcactgc cagccgaggg ccggcgagac ggtggtggtg tccgccgcct cgggcgcggt
     2641 gggcagcgtg gtggggcaac tggcgaagat gcagggctgc cgcgcggtgg gcatcgccgg
     2701 cggcagcgcg aagtgcgatt acgtggtgcg cgagctgcgc ttcgacgcct gcgtcgacca
     2761 caaagggggc aagctgtacg acgacctcaa ggcggcctgt ccggaaggca tagactgcta
     2821 cttcgagaac gtcggcggcg aggtgatgga caccgtgttc cgccagctca acccgttctc
     2881 gcgcgtggcg ctgtgcggca tgatcgccga ctacaacctg accgagccgt acggcgtgaa
     2941 gatgctgcgc gcgctgctga tgaaccgggt gaagctgcag ggtttcatcg tctccgaccg
     3001 catggacctg taccagcgcg cgctgcagcg gctcggcaag gcggtggccg agggcaagat
     3061 caagtatcgg gaaacggtgg cgcagggact ggagaacgcg cccaaagcct tcatcggcat
     3121 gctgaagggc gccaacctcg gcaagcagct ggtaaaggtg gcgtgatccg ctacacgcgc
     3181 cggccgacga ccaccagatc gcgctccacg ccgtccagcc gcgccacgcc cggcatgaag
     3241 ccccagcgct cgaagccgaa cttcgcgaac agccgcaggc tcggcgcgtt gtgaccgacg
     3301 atgaagccca gcagccgatc gacgccgatg gagggcgcct gcgcgagcgc cgcgcgcagc
     3361 aggtaagaac cgaggccctg cccgcgcgca cgctggtcca cgtagacgct gagctcggcg
     3421 gtcttgtcgt aggccggcct gccgtagaag ctggagaagc tcagccaggc c
//
LOCUS       DASPGE010000031        12309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1413687, whole genome shotgun sequence.
ACCESSION   DASPGE010000031 DASPGE010000000
VERSION     DASPGE010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12309
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413687"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VD791_00925"
     CDS             <1..1065
                     /locus_tag="VD791_00925"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXV06566.1"
                     /translation="RDYTIYFQQLHRSQLPLALRLEADRMANLVLTQAEFAREIRVVM
                     EERRLRTEDQPRSLLYETFLATAYQAYPYRTPIIGWMRDLENMTLVDARTWYQTWYAP
                     NNAALVVVGDVQAADVFAEAQRWFGAIAPRALPERKAQSEAPQRGIRRAVVNAPAELP
                     YLLMGWHVPALRDLERDWEPYALWVLAAALDGSDASRLTRDLVREQRLAVSADASYDP
                     VNRGPGLFVVSATPAAGHSAEALEAALRAQIVAIGERGITAEELERTKVQAVAAQVYG
                     QDSILGQARQIGRLHNAGLPPDGAAVQVRRLQEVTVAQVQAVARKYFADDNLTVGTLR
                     PQPLAAAARAGAIDAPLREH"
     gene            1127..2461
                     /locus_tag="VD791_00930"
     CDS             1127..2461
                     /locus_tag="VD791_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXV06567.1"
                     /translation="MRLRVEILAAAALALAASAALGALPIQHWQTSSGARVYFVEGRN
                     LPILDLSVEFPAGSSRDTPATSGLAGLTLRMLRFGAQGFTEEQISLRLADVGAQLASS
                     FDYDRAGYALRTLSGAAQREQALATLAALLQRPSFPAAVLEREKARVIAGLREAESKP
                     DAIGAREFARLVYGGHPYGLRESGEPQTVARLTREDLAAFHARFFAAPYAVVAMIGDL
                     DRQEAEAVAERLVAGLPKGAGPLPPLPPVPRLDAPVQRVIAHPAAQAHLYLGAPGVRR
                     IDPDYFPLLVGNYILGGGGFNSRFSEQVRQKRGLSYSVYSRLVPYEQDGAFTVGLQTR
                     KDQAGEALRVVRDTFADFVANGPSAEELDGARQHIVGAFPLRIDSNRKILEHLAVIGF
                     YRLPLDWLERYPQSVTAVTAAQIRDAFQRRISPERLAVVVVGGPWDGSPVPP"
     gene            2434..3054
                     /gene="rsmD"
                     /locus_tag="VD791_00935"
     CDS             2434..3054
                     /gene="rsmD"
                     /locus_tag="VD791_00935"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HXV06568.1"
                     /translation="MGRKSCPAVSRAGPPNRVRIVGGKWRSRVLRFPDLPGLRPTPDR
                     VRETLFNWLGQDLTGRVCLDLFAGSGVLGLEAASRGAREVVMVERDPQALRALQDNIG
                     MLQATAVRLVRADALEFIGSDARSYDVVFLDPPYAMGGRERLLERVRERVAAGGLVYV
                     EGPEDVRAPAGWAVHRAARAGAVHYQLLIAVDRSWIKPETDRHETR"
     gene            3103..3585
                     /gene="coaD"
                     /locus_tag="VD791_00940"
     CDS             3103..3585
                     /gene="coaD"
                     /locus_tag="VD791_00940"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HXV06569.1"
                     /translation="MKKAVYPGTFDPVTRGHEDLVRRAARLFDHVILAVADSRGKRPF
                     FSLEERVDMARSVLTGCDNVEVMGFSGLLMDFMRQHNAKIILRGLRAVSDFEYEFQMA
                     GMNRQLYPDVETVFLTPAEQYMFISATIVREIATLGGDVAPFVNPLILHRLTRMNGAD
                     "
     gene            complement(3582..4397)
                     /gene="mutM"
                     /locus_tag="VD791_00945"
     CDS             complement(3582..4397)
                     /gene="mutM"
                     /locus_tag="VD791_00945"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HXV06570.1"
                     /translation="MPELPEVETTRRGVEPWLLNQRIREAVLRHRGMRWPVRADLSRL
                     LAGHRIRRVDRRSKYLLLDCEAGWLILHLGMSGSLRIVPAQQAPGKHDHFDLVLDSGR
                     ALRLTDPRRFGAVLWQAGEPARHKLLRGLAPEPFDAAFDADWLYRRTRGRSAAIKSVL
                     MDSRVVCGVGNIYANEALFRAAIRPARAAGRISHSRCAGLVDAVRATLREAIEAGGSS
                     LRDFVHADGSSGYFQQRYFVYGREGEPCRRCAAPIKALRIGQRSAFYCPRCQR"
     gene            complement(4402..5352)
                     /locus_tag="VD791_00950"
     CDS             complement(4402..5352)
                     /locus_tag="VD791_00950"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HXV06571.1"
                     /translation="MATPARLSAFATAMSPMRIGWIVNLLLLVGVVGLAAYALWGRKA
                     EDPPGHAIAAIKAAEIKRVVIEPREGKGMILEKQGEDWFVAQPLQARADRGQVERLLD
                     LLSAKSRERLTAADLQRFELDTPDLKVSFDGHSVAFGTTNPLSQDQYVLSGDGVYLLP
                     AYYRSLVPESPERVLTHALFRGDEKPVGFALGRFRVEQRDAKWTLSPAPADAQNRPTA
                     DDLNRWVDEWRLASSLLTQPATRKPAAETIPVETIEVRLADGRTLRLGVRQREPELVL
                     VRADENLSFHFSGEMARRLLNPPAATPAPDAGAASAAQPQ"
     gene            complement(5331..6689)
                     /locus_tag="VD791_00955"
     CDS             complement(5331..6689)
                     /locus_tag="VD791_00955"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HXV06572.1"
                     /translation="MKASRRMQVQLLAQNTLFGALLVAAAVLGVYLLDDVKLQRDVTQ
                     NKRGSLSQATRDVLGRMQGPIVITAYATHQDPVLGDVRRVIHDFVAPYIQVKPDIGLE
                     FIDPREQPKQTAAANVRANGELVIEYGKRSEHLTSLNEQNMANLLMRLARSQERLIMH
                     VDGHGEPKLDGGANFDLGEFGRQLGTKGFRVQGLNLLVAPEVPDNVDVLVVTHPRTQM
                     IQGEVSKIIRHLDRGGSLLWLVDQEPLRGLQSLAEYLKVELTPGVVVDPAAAQLGIQP
                     TIALSSNYGFHPVTENFTQYNTAFPFARAIGRPAENGEWRATALVEVAQNGWVETGDL
                     RASLRFDKDRDVRGPVAVAVALEREVKGKPQRVVAVGGSGFLTNAFVGLLSNLDLGTN
                     MLNWLAEDESLITIQPRARVDSTLELSRAALIAIVTAFLLALPAAFLFAGGAIWWRRR
                     RA"
     gene            complement(6694..7437)
                     /locus_tag="VD791_00960"
     CDS             complement(6694..7437)
                     /locus_tag="VD791_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXV06573.1"
                     /translation="MIRAIALKEWRLLFASPLAWVVLAFLQLVFAWVFLSRLNAFLEL
                     QPQLATTPGAPGLTEIVAAPVFGTATIILLMVVPLLSMRLVAEERRSQTLPFLISAPV
                     SITQIVLGKFLGLLGILALAVGLIVAMCASLYLGGRLDLGLLAANALGLLLLAGTFAA
                     VGLYLSCLTAQPLVAAVGTFAVLLALWLINISATDPGSLLHVLSLIQHFESFSKGTLA
                     LKDLGYYLVLIALFLLLSIRRLDADRLRA"
     gene            complement(7434..8492)
                     /locus_tag="VD791_00965"
     CDS             complement(7434..8492)
                     /locus_tag="VD791_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV06574.1"
                     /translation="MSDNSASPADTSAPPAELTLAARDLSRHFGTTPAVAGIDLELRR
                     GEVLGLLGPNGAGKTTTMQMLTGNLAPTTGSISVCGIDLLERPTAAKARIGYLPEVPP
                     LYKELKVDEYLRLAARLHRVPRSETAAALERAKRRCGVSDIGHRLIGTLSKGYQQRVG
                     IAQAVIHSPDLVILDEPTVGLDPNQIREIRALIRELSDSHSVILSTHILPEVEAVCDR
                     VQILHRGRIVYNDRIAALQRFRGAHSVLVGFRRPPSVDEISRLPGVAGVEPAAEGLLR
                     VLRTGAPAATDDAVPSGAPDLIDAPDLIDTLVRLSVQRNWALTHLSIERASLEDVFVH
                     LTRQEAPEAAAAAAEATA"
     gene            8815..10635
                     /locus_tag="VD791_00970"
     CDS             8815..10635
                     /locus_tag="VD791_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV06575.1"
                     /translation="MPFALRSILPYSVLLLAACAQQPVLGPIGFPPHSLTASAQDVPL
                     PARLAQQSAEEESLEERARRIFANQPPPASNLPDTALDQELLFKFLLSEIAGQRGNMQ
                     LAAQGYLEMARATRDPRLARRAAEIATYARLQGVALESARLWYELDKGNPQARQSLVG
                     LLLSGNKLGEAKPHLQAIVNADGNPAQGFMQLHSLMSKHPDKQAVLAVVKDLAQGYKQ
                     MPEAHFAVAQSAYAAGKHELALAEVREAMRLRPEWEIGALLQAQMLQQAQSTQKALEY
                     LRGFLDGHPKAREARANYARLLINDKQLEQARAQYQIMLQEQPANADVVVTIGLLSLQ
                     LDDFEAAETNLKRGLELNYRDPDTLRFYIGQSYEERKRYRDAMHWYTQVAEGDQYIAA
                     QARYAFLLGRDNKLAEARQYLQNVKVQSDEQRAVLIQAEAQLLREARNYRESYDLLAG
                     ALEKLPENADLLYDSALAAEKLDKLDVVETNLRKLIKLKPDHAQAYNALGYTLADRTD
                     RLVEAKEYIEKALALAPEDPFILDSMGWVHFRLGDHQEGLTYLERAYKVRPDPEIAAH
                     LGELLWVRGRQSDAEKLWRESQKEHPDNELLLDTMNRFLK"
     gene            10635..11222
                     /gene="lolB"
                     /locus_tag="VD791_00975"
     CDS             10635..11222
                     /gene="lolB"
                     /locus_tag="VD791_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HXV06576.1"
                     /translation="MPSRLRAAVLAAPFAALLCACAAVPRGPEISPVDPAALVSFELN
                     GRINLKVQKEAFPGRVRWQHAPAMDELWFYTPVGNAVAHLRQDGDGAWLMTAEGREHR
                     ADDLERLAFEVLGWDLPLQALPYWVRGIPWPQADAADTQRDGQGRLRRLQQGAWQVTY
                     LAWGASGLPVKLDLLGERLRLRLVVDRWSAHATGG"
     gene            11206..12060
                     /gene="ispE"
                     /locus_tag="VD791_00980"
     CDS             11206..12060
                     /gene="ispE"
                     /locus_tag="VD791_00980"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HXV06577.1"
                     /translation="MPPAVESGGVSCSAPAKLNLFLHVIGRRADGYHWLQTLFRFLDY
                     GDTLSVSIRPDGAVRRIGDVPGVSEEQDLALRAARLLQAASATTWGADIALDKRLPPG
                     GGLGGGSSDAATALIALNRLWGLRWTRARLQELALQLGADVPVFVFGRSAFAEGVGER
                     LQAVTLPPAWYLVLVPSVSVSTATVFADPELTRNSDPIKIHGFSAGPGRNDLEGVVCR
                     RYAQVAQCLDWLRQFGPAAMTGSGASVFAAFDTEQAAQQVFRRRPAGVQGFVARGLDR
                     HPLWKWVE"
     gene            12087..12163
                     /locus_tag="VD791_00985"
     tRNA            12087..12163
                     /locus_tag="VD791_00985"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12121..12123,aa:Gln,seq:ttg)
BASE COUNT         1883 a         4257 c         4318 g         1851 t
ORIGIN      
        1 cgcgactaca ccatctattt ccagcagctg caccgctcgc agctgccgct cgccttgcgg
       61 ctggaggccg accgcatggc caacctggtg ctcacgcagg cggagttcgc gcgcgagatc
      121 cgggtggtga tggaggagcg gcgcctgcgc acggaggacc agccgcgttc tctgctgtac
      181 gagacgtttc tcgccaccgc gtatcaagcg tacccctacc gcacgccgat catcggctgg
      241 atgcgggacc tggagaacat gacgctggtc gatgcgcgca cctggtatca gacctggtac
      301 gcaccgaaca acgccgcgct ggtggtggtg ggcgacgtgc aggccgcgga cgtgttcgcc
      361 gaagcgcagc gctggttcgg cgccatcgcg ccgcgcgcct tgcccgagcg caaggcgcag
      421 agcgaggcgc cgcagcgcgg catccggcgc gccgtggtca acgcgccggc cgagctgcct
      481 tatctgttga tgggctggca tgttcccgcg ctgcgcgatc tggagcgcga ctgggagccg
      541 tacgcgctgt gggtgctggc agcggccctg gacggcagcg acgcatcgcg cctgacgcgc
      601 gacctggtgc gcgagcaacg gctggcggta tcggccgacg ccagctacga tccggtcaat
      661 cgcgggcccg gcctgttcgt ggtgtccgcc acgccggccg cgggtcacag cgccgaagcg
      721 ctggaggcgg cattgcgtgc gcagatcgtg gccatcgggg agcgcggcat caccgccgag
      781 gagctggaac gcaccaaagt gcaggcggtg gccgcgcagg tatacgggca ggattccatc
      841 ctcggccagg cgcggcagat cggcaggctg cacaacgccg gcctgccgcc cgatggagcc
      901 gcggtgcagg tgcgcaggct gcaggaggtc acggtggcgc aggtgcaggc ggtcgcgcgc
      961 aagtacttcg ccgacgacaa tctcaccgtc ggcacgctcc ggccgcaacc cctcgccgcg
     1021 gctgcgcgcg ccggcgcaat cgacgccccg ctgcgtgaac actgacaatt tcccgggccg
     1081 gttctgctcg aggtggcccg cgcccgcgcg gctgcgttca ccccgcttgc gcctccgcgt
     1141 cgagatcctc gcggcggccg cgctcgcgct ggccgcgtcc gccgcgctgg gcgccctgcc
     1201 gatccagcac tggcagactt ccagcggcgc gcgcgtctac ttcgtcgagg gccgcaatct
     1261 gccgatcctc gacctgagcg tggagttccc ggcgggatca agccgcgaca cgccggcgac
     1321 ctcggggctt gccggcctca ccctgcgcat gctgcgcttc ggcgcgcaag ggttcaccga
     1381 ggagcagatc tcgctgcggt tggcggacgt cggcgcccag ctcgcttcga gcttcgatta
     1441 cgaccgcgcc ggctatgcgc tgcgcacgtt gagcggcgcg gcgcagcgcg agcaggcgct
     1501 cgccacgctc gccgccctct tgcagcggcc cagtttcccg gccgccgtgc tggagcggga
     1561 gaaggcgcgc gtgatcgccg gcctgcgcga ggcggagtcg aagcccgacg ccatcggcgc
     1621 gcgcgagttc gcgcgcctgg tctacggcgg gcatccctac ggcctgcgcg agtcgggcga
     1681 accgcaaacg gtggcgcggc tcacccgcga ggacctcgcc gcgtttcatg cgcgcttctt
     1741 cgcggcgccc tacgccgtgg tggcgatgat cggcgacctc gaccggcaag aggccgaggc
     1801 cgttgccgag cgcctcgtcg ccgggctgcc gaagggcgcc ggcccgctgc cgccgctgcc
     1861 tccggtgccg cgcctggacg ccccggtgca gcgcgtgatc gcccacccgg ccgcgcaggc
     1921 gcatctgtac ctgggcgctc cgggcgtgcg ccgcatcgac cccgactact ttccactgct
     1981 ggtgggcaat tacatcctgg gcggcggcgg cttcaactcg cgcttcagcg agcaggtgcg
     2041 gcagaagcgc ggtctgtcct acagcgtcta cagccggctg gtgccctacg agcaggatgg
     2101 cgcgttcacc gtcggcttgc agacgcgcaa ggatcaggcc ggcgaggcgc tgcgcgtggt
     2161 gcgcgacacg ttcgccgact tcgtggccaa cggacccagc gccgaggagc tggacggcgc
     2221 ccgccagcac atcgtgggcg cgttcccgct gcgcatcgac agcaaccgca agatcctcga
     2281 gcaccttgcg gtgatcggtt tctaccgcct cccgctcgac tggctggagc gctatccgca
     2341 gagcgtgacc gcggtgacgg cggcgcagat ccgcgacgcc ttccagcggc gcatctcgcc
     2401 cgagcgcctg gccgtggtgg tggtgggcgg gccgtgggac ggaagtcctg tcccgccgtg
     2461 agccgggccg ggccgcccaa ccgggtgcgc atcgtgggcg gcaagtggcg cagccgcgtg
     2521 ctgcgctttc ccgatctgcc ggggctgcgc ccgacgcccg accgcgtgcg cgagacgctg
     2581 ttcaactggc tgggacagga tctcaccggc cgggtgtgcc tcgatctgtt cgccggcagc
     2641 ggcgtgctgg gcctggaagc cgcctcgcgc ggcgcgcgcg aggtggtgat ggtcgagcgc
     2701 gacccgcagg cgctgcgcgc gctgcaggac aacatcggca tgctacaggc cacggccgtc
     2761 cgactggtgc gggcggatgc gctagaattc atcggatcgg acgcccgcag ctacgacgtc
     2821 gtgttcctcg atccgcccta cgcgatgggc ggtcgcgagc ggctcctgga acgcgtgcgc
     2881 gagcgggtcg ccgccggcgg gctggtgtac gtggaagggc cggaggatgt gcgcgcgccg
     2941 gccggctggg cggtacatcg cgccgcacgc gccggcgccg tgcactatca gcttctgatc
     3001 gccgtcgacc gatcgtggat caagcccgaa actgatcgtc atgaaaccag gtgaaccgat
     3061 ggtgaaggcg gtggtgaagc caatggcgag catggcggcg tcgtgaagaa ggcggtctat
     3121 cccgggactt tcgaccccgt cacccgcggt cacgaggacc tcgtgcgccg tgccgcccgc
     3181 ctgttcgatc acgtcatcct ggcggtggcc gacagccgcg gcaagcgccc gttcttcagc
     3241 ctcgaggagc gcgtcgacat ggcgcgcagc gtgctcaccg gctgcgacaa cgtcgaggtg
     3301 atgggcttct ccgggctgct gatggacttc atgcgccagc acaacgcgaa gatcatcctg
     3361 cgcgggttgc gcgcggtgtc cgacttcgag tacgagttcc agatggccgg catgaaccgc
     3421 cagctctatc ccgacgtcga aaccgtgttc ctgacgcccg ccgagcagta catgttcatc
     3481 tcagccacca tcgtgcgcga gatcgccacc ctgggcgggg acgtcgcccc gttcgtcaac
     3541 ccgctgatcc tgcaccgtct cacgcgcatg aacggcgccg actagcgctg gcagcgcggg
     3601 cagtagaacg ccgagcgctg gccgatgcgc agcgccttga tcggcgccgc gcagcgccgg
     3661 caaggctcgc cctcgcgccc gtagacgaag tagcgctgct gaaagtatcc ggagctgccg
     3721 tcggcgtgca cgaagtcgcg caggctgctg ccgcctgcct cgatcgcctc gcgcagggtc
     3781 gcgcgcaccg cgtcgaccag acccgcgcag cggctgtgcg agatgcgccc ggcggcgcgc
     3841 gccgggcgga tggccgcgcg gaacagggcc tcgttggcgt agatgttgcc cacgccgcac
     3901 accacgcggc tgtccatcag cacgctcttg atcgccgcgc tgcgcccgcg cgtgcgccgg
     3961 tacagccagt cggcgtcgaa cgcggcgtcg aacggctcgg gcgcgagccc ccgcagcagc
     4021 ttgtgccgcg ccggttcgcc cgcctgccac aggaccgcgc cgaagcgtcg cgggtcggtc
     4081 aagcgcaacg cccggccgct gtcgagcacc aggtcgaagt gatcgtgctt gccgggtgcc
     4141 tgctgcgcgg ggacgatgcg caggcttccc gacatgccca gatgcaggat cagccacccg
     4201 gcttcgcagt cgagcaggag gtacttgctg cgccggtcca cgcggcggat acggtgcccg
     4261 gccagcaggc gcgacaggtc cgcgcgcacc ggccagcgca tgccgcgatg gcgcagcacc
     4321 gcttcccgga tgcgctggtt cagcagccag ggctcgacgc cccggcgcgt ggtttcgacc
     4381 tcgggcagct cgggcatgcg cttactgcgg ctgcgccgcc gaagccgcgc cggcgtcggg
     4441 cgcgggcgtg gccgccggcg ggttgagcag gcgccgggcc atctcgccgg aaaagtggaa
     4501 gctcaggttc tcgtccgccc gcaccagcac cagctcgggc tcgcgttggc gcacgccgag
     4561 ccgcagcgtc cggccatcgg ccagccgcac ctcgatcgtt tccaccggga tcgtttccgc
     4621 cgccggtttg cgggtcgccg gctgcgtgag caggctggaa gcgagtcgcc actcgtccac
     4681 ccagcgattg agatcgtccg ccgtgggccg gttctgcgcg tccgcaggcg ccggagacag
     4741 cgtccatttc gcgtcgcgct gctcgacgcg gaagcgcccg agagcgaacc ccaccggctt
     4801 ctcgtcgccg cggaacagcg cgtgcgtgag cacgcgctcg ggactctcgg gcaccaggga
     4861 gcggtagtag gccgggagca ggtacacgcc atcgccggac agcacgtact ggtcctgcga
     4921 cagcggattg gtggtgccga aggccacgct gtggccgtcg aagctcacct tgaggtcggg
     4981 cgtgtcgagc tcgaagcgct gcaggtccgc ggcagtcagc ctttcccggc tcttggccga
     5041 gagcagatcg agcagccgct ccacctggcc gcggtcggcg cgcgcctgca gcggctgcgc
     5101 cacgaaccag tcctcgccct gcttctccag gatcatgccc ttgccctcgc gcggttcgat
     5161 aaccacgcgc ttgatctctg cggccttgat ggcggcgatg gcgtgtcccg ggggatcctc
     5221 ggccttgcga ccccagaggg cgtaggcggc gagcccgacc acgccgacca gcagcagcag
     5281 gttgacgatc cagccgatgc gcatcgggct catggctgta gcgaaggcgc tcaggcgcgc
     5341 cggcgtcgcc accagatcgc gcctccggcg aacaggaatg cggccggcag agccagcagg
     5401 aaggcggtga caatggcaat gagcgcggct ctggacaact ccagcgtgct gtccacgcgc
     5461 gcgcgcggct gaatcgtgat caggctctcg tcctcggcca gccaattgag catgttggtg
     5521 cccaggtcga gattgctgag cagcccgacg aatgcattgg tgaggaagcc gcttcccccc
     5581 accgcgacga cgcgctgcgg cttgcctttc acttcgcgct ccagcgccac tgccaccgcc
     5641 accgggccgc gcacatcgcg atccttgtcg aagcgcaggc tggcccgcag gtcgccggtc
     5701 tcgacccagc cgttctgcgc cacttccacc agcgcggtcg cgcgccattc gccgttctcc
     5761 gcaggacggc cgatcgcgcg ggcgaacggg aacgcggtgt tgtactgggt gaagttctcg
     5821 gtgaccgggt ggaagccgta gttggaggac agcgcgatgg tgggctggat gccgagctgc
     5881 gcggcggcgg gatccaccac cacgccgggg gtcagctcga ccttcaggta ttcggccagc
     5941 gactgcaggc cgcgcagcgg ctcctggtcc accagccaca gcaggctgcc gccgcgatcg
     6001 aggtgacgga tgatcttgct cacctcgccc tggatcatct gcgtgcgcgg atgggtgacg
     6061 accagcacat ccacgttgtc cggaacctcc ggtgcgacca gcaggttcag cccctgcacg
     6121 cggaagccct tggttccgag ttgccgcccg aactcgccca ggtcgaagtt ggcgccgccg
     6181 tccagcttcg gctcgccgtg cccgtccacg tgcatgatca ggcgctcctg gctgcgcgcc
     6241 aggcgcatca gcaggttggc catgttctgc tcgttcaggc tggtgaggtg ctcggagcgc
     6301 ttgccgtact cgatcaccag ctcgccgttg gcgcgtacgt tcgcggcggc ggtctgcttg
     6361 ggctgctcgc gcggatcgat gaactcgagc ccgatgtcgg gcttcacctg gatgtagggc
     6421 gcgacgaaat cgtggatcac gcggcgcacg tcccccagga ccggatcctg atgggtggcg
     6481 taagcggtga tgacgatcgg cccctgcatc ctgcccagca cgtcgcgcgt ggcttgagac
     6541 agactgccgc gcttgttctg ggtgacgtcc cgctgcagct tgacgtcgtc gagcaggtac
     6601 acgcccagaa cggctgcggc caccagcagc gcgccgaaca gcgtgttctg cgccagcagc
     6661 tgcacctgca tgcgacggct cgctttcatg ccttcacgcg cgcagacggt cggcgtccag
     6721 acgccggatg gaaagcagca ggaacagcgc gatcagcacc aggtaatagc cgaggtcctt
     6781 gagcgccagc gtgcccttgg agaagctctc gaagtgctgg atcagggaca gcacatgcag
     6841 caggctgccc gggtcggtcg ccgagatgtt gatcagccac agtgcgagca ggacggcgaa
     6901 ggtgccgact gcggccacca gcggctgggc ggtcaggcag gacaggtaca acccgactgc
     6961 ggcaaaggtg ccggcgagaa gcagcaggcc gagcgcgttg gccgccagca gccccaggtc
     7021 cagccggccg ccgaggtaga gcgaggcgca catggcgacg atcaacccca ccgccagcgc
     7081 caggatgccg agcagcccga ggaacttgcc gagcacgatc tgggtgatcg acaccggtgc
     7141 cgagatcagg aaaggcagcg tctggctgcg ccgctcctcg gccaccaggc gcatggagag
     7201 cagcggcacc accatcagca ggatgatggt ggcggtgccg aacaccggcg cggccacgat
     7261 ctcggtgagc ccgggtgcgc ccggcgtggt cgcgagctgg ggctgcagct cgaggaaggc
     7321 gttcagccgg gacaggaaca cccaggcgaa caccagttgc aggaaggcca gcaccaccca
     7381 ggcgagcggg gaggcgaaca acagccgcca ctctttcagc gcgatcgccc ggatcacgcc
     7441 gtcgcctccg cggctgccgc cgccgcctct ggagcctcct ggcgggtcag gtgcacgaac
     7501 acgtcctcca ggctggcgcg ctcgatcgac aggtgcgtga gcgcccagtt gcgctgcacc
     7561 gacagacgca ccagggtatc gatcagatcc ggggcatcga tcagatccgg agcgccgctg
     7621 ggaacggcgt catcggttgc tgcgggcgcg cctgtgcgca ggacgcgcag caacccctcg
     7681 gccgccggct cgacgcccgc cacgccgggc aggcgcgaga tctcgtccac ggacggcggg
     7741 cggcggaagc cgaccagcac actgtgcgcc ccgcggaacc gctgcagcgc cgcgatgcgg
     7801 tcgttgtaca cgatccggcc acgatgcagg atctgcacgc ggtcgcatac cgcctccacc
     7861 tccggcagga tgtgcgtgga caggatcacg ctgtggctgt cggacagctc gcggatcaac
     7921 gcgcggatct cgcggatctg gttcgggtcc agccccactg tgggctcgtc caggatcacc
     7981 aggtcggggc tgtggatcac ggcctgtgcg atccccacgc gctgctggta gcccttggac
     8041 agggtcccga tcaggcgatg cccgatgtcg ctcacgccgc agcggcgctt ggcgcgctcc
     8101 agcgccgcgg cggtctccga ccgcgggacc cggtgcaggc gggcggcgag ccgcaggtat
     8161 tcgtcgactt tcaactcctt gtaaagcggg ggcacctcag gcaggtagcc gatacgcgcc
     8221 ttggcggcgg tggggcgctc cagcaggtcg atgccgcaga cgctgatgct gcccgtggtc
     8281 ggcgcgaggt tgccggtgag catctgcatg gtggtggtct tgccggcgcc gttcggcccg
     8341 agcaggccca gcacctcgcc gcgccgcagc tcgaggtcga tgcccgccac cgccggggtg
     8401 gtgccgaaat ggcgcgacag atcccgcgcg gccagggtca gctccgctgg aggcgcagag
     8461 gtatcggccg gagatgcaga attatccgac attcgtcaaa gggttgtgcg gcaaagaact
     8521 tgcttcgacc ggcggcaggc acgccgctag gcgctgatcc aacctgcgct ccgaccggct
     8581 ccgccgaagt cggttccccg cgcctgccgg gggaacgcag ggggctcggg ccgatgcgac
     8641 acgagtgact tgaggcgcgg cgcaaatata cctaaactga ccgtgatgtt gaaggcgccg
     8701 gcagcgggct tatttcgagg cgcgccacgg ttttcccagc acctcgttgc cttgacgcaa
     8761 ttggccgctg atcagcattg cctgatccac tgactgaagc cgggaagcac cgccatgccc
     8821 ttcgccctcc gctccatact cccgtacagc gtgctcctcc tggccgcctg cgcccagcag
     8881 ccggtgctgg ggccgatcgg cttcccaccc catagcctga ccgcgtcggc ccaggacgtg
     8941 ccgctgccgg cgcgcctcgc gcagcagtcc gccgaggagg aatcgctgga ggaacgggcg
     9001 cggcggatct tcgcgaatca gcccccgccc gcgtccaacc tgcccgatac cgcgctcgat
     9061 caggagctgc tgttcaagtt cctgctctcc gagatcgccg gtcagcgcgg caacatgcag
     9121 ctcgccgcgc agggctatct ggagatggcc cgggcaaccc gcgatccgcg cctggcgcgc
     9181 cgggccgccg agatcgcgac ttacgcccgg ctgcaggggg tcgcgctgga gtccgcgcgc
     9241 ctgtggtacg agctggacaa gggcaatccg caggcgcggc agagcctggt gggactgctg
     9301 ttgtcgggca acaagctggg cgaggccaag ccgcacctgc aggcgatcgt caacgcggac
     9361 ggcaatcccg ctcagggctt catgcagctg catagcctga tgtccaagca cccggacaag
     9421 caggccgtgc tcgcggtggt gaaggacctg gcgcaaggct acaagcagat gccggaggcg
     9481 catttcgcgg tggcgcaatc ggcctacgcc gccggcaagc acgagctggc gctcgccgag
     9541 gtgcgcgagg cgatgcggct gcgccccgag tgggagatcg gtgcgctgct gcaggcgcaa
     9601 atgctgcagc aggcgcagtc cacgcagaag gcgctcgaat acctgcgcgg tttcctcgac
     9661 gggcatccga aggcgcgcga ggcgcgcgcc aactatgcgc gtctgctgat caacgacaag
     9721 cagctggagc aggcgcgcgc gcagtaccag atcatgctgc aggagcagcc cgccaacgcc
     9781 gacgtcgtgg tcaccatcgg gctgctgtcc ctgcagctcg acgacttcga ggccgccgag
     9841 acgaacctca agcgcgggct cgagctcaac taccgcgatc ccgacaccct gcgcttctac
     9901 atcggccagt cgtatgagga gcgcaagcgc tatcgcgacg ccatgcactg gtacacgcag
     9961 gtcgccgagg gcgatcagta catcgccgcc caggcgcgct acgccttcct gctggggcgc
    10021 gacaacaagc tcgccgaggc gcgccagtac ctgcagaacg tcaaggtgca gagcgacgag
    10081 cagcgcgcgg tcctgatcca ggccgaggcc cagctgctgc gcgaggccag gaactaccgc
    10141 gagtcctacg acctgctggc cggggcgctg gagaagctgc cggagaacgc cgacctgctc
    10201 tacgacagcg cgctcgccgc cgagaagctg gacaagctcg acgtggtcga aaccaacctg
    10261 cgcaagctga tcaagctcaa gcccgatcat gcgcaagctt acaacgccct ggggtacacg
    10321 ctggccgatc gcaccgatcg cctggtcgag gccaaggaat acatcgaaaa agccctggcg
    10381 ctcgcgcccg aggacccgtt catcctggac agcatgggct gggtgcactt ccgcctgggc
    10441 gaccaccagg aagggctgac ctacctggag cgcgcctaca aggtgcgccc cgatccggag
    10501 atcgccgccc acctgggcga gctgctgtgg gtcagggggc ggcagtccga cgccgagaag
    10561 ctctggcgcg agtcgcagaa ggagcatccg gataacgagc tcctgctcga cacgatgaac
    10621 cgcttcctga agtagtgccc tcacgcctgc gcgccgcggt cctcgcggcg ccattcgccg
    10681 cgctgctgtg cgcctgcgcc gccgttccgc gcgggccgga gatctctccc gtcgatcccg
    10741 cagcgctggt gagcttcgag ctgaacgggc gtatcaacct caaggtgcag aaggaagctt
    10801 tccccggccg cgtgcgctgg caacacgcac ccgcgatgga cgagctgtgg ttttatacgc
    10861 cggtgggcaa cgccgtggcg cacctgcggc aggacggcga cggcgcctgg ctgatgactg
    10921 cggaggggcg cgagcaccgc gccgacgatc tggagcggct ggccttcgag gtgctgggct
    10981 gggatctgcc gctgcaggcg ctgccctact gggtgcgcgg catcccctgg ccgcaggccg
    11041 acgcggccga tacgcagcgc gacgggcagg gccgcctcag gcggctgcag cagggagcct
    11101 ggcaggtgac ctacctcgcc tggggggcga gcggcctgcc ggtaaagctc gacttgctgg
    11161 gcgagcgcct gcgcctgcgc ctggtcgtgg accgctggag cgcgcatgcc accggcggtt
    11221 gagtcgggcg gtgtgagctg ctccgcgccc gccaagctca acctgttcct gcacgtgatc
    11281 ggccgccgtg ccgacggcta ccactggctg cagacgctgt tccgcttcct cgactacggc
    11341 gatacgctct cggtgagcat tcgcccggac ggtgccgtac ggcggatcgg cgacgtgccc
    11401 ggcgtgagcg aagagcaaga cctcgcgctg cgagcggcgc gcctgctgca ggcggcgtcg
    11461 gcaacgacct ggggcgccga tatcgcgctc gacaagcgct tgccgcctgg cggagggttg
    11521 ggcggcggca gctccgatgc cgccaccgcc ctgatcgcgc tcaatcgcct gtggggcctg
    11581 cgctggacgc gcgcccgcct gcaggagctc gcgctgcagc tcggcgccga tgtgccggtc
    11641 ttcgtgttcg gccgcagcgc tttcgccgaa ggggtgggcg agcggctgca ggcggtcacc
    11701 ctgccgccgg cgtggtacct ggtgctggtc ccgtcggtga gcgtttcgac tgcgacggtc
    11761 ttcgccgatc ccgaattgac acgcaatagc gatcccatca aaatacacgg cttttctgca
    11821 ggccccggcc gaaacgacct ggaaggcgtg gtatgccgcc gctacgcgca agtggcgcag
    11881 tgtctggact ggctccggca attcggtcct gcagcgatga ccggttcggg cgcgagcgtg
    11941 ttcgccgcct tcgacacgga acaggcggca caacaggttt tccggcgccg cccggcgggt
    12001 gtgcagggtt tcgtggctcg cggactggac cgtcacccgt tgtggaaatg ggtcgagtag
    12061 ccgcgcgtcc cggcgcgagg cgcgaatggg gagtcgccaa gctggttaag gcaccggatt
    12121 ttgattccgg catgcgtagg ttcgaatcct acctccccag ccaagagcag gggctaggag
    12181 ctgggatcta gggcgtgtgc agtcatgccg acttcgccgc acaaagcttc cgcttgcgtt
    12241 atcgtgacac gatcctccct agatcctaga tcctagatcc tcccgtcatg gcctacgaca
    12301 gcttgatgg
//
LOCUS       DASPGE010000032         6666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_244160, whole genome shotgun sequence.
ACCESSION   DASPGE010000032 DASPGE010000000
VERSION     DASPGE010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6666
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244160"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1073
                     /locus_tag="VD791_00990"
     CDS             282..1073
                     /locus_tag="VD791_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HXV06578.1"
                     /translation="MPYDRPPLKIGLSPRFLYKSPLELGFRGKTLLYLEQSIAHWVMS
                     HGALIFMIPSIETGGLIRRGNLSVEDYVAALDGLVLQGGTDVSPRSYGHEPLRPEWQG
                     DIVRDQYEMELLAGFVDKGKPVLGICRGAQLINVAFGGTLWQDIPSMVPAALRHHDTE
                     LYDENFHPLRFEPGSLLEKIFPGQRSGLVNSIHHQAARDLGAGLQVEGRGDADGVVEA
                     IRWDGPSFVYGMQWHPEFHPGREGLLDCTPILEAFLREARQRMRG"
     gene            1222..2061
                     /locus_tag="VD791_00995"
     CDS             1222..2061
                     /locus_tag="VD791_00995"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06579.1"
                     /translation="MNQLRSLGDALAIVGSTLLERFVVFVPSVIGAVLLLLAGWVLAR
                     LLRVLATRAAMLLDTLLARVGGPAGAERLRMGRAAGMLGTGVFWVVLLFFATAATQVL
                     GLQTFTDWLARLIDYLPTLAAGVLIVVVGYVVSRVVGDVVRATATRLDAAQRSVLARL
                     TQVAILVGAVLVGADQIGIRITFLAIFAGAAAAAVVGGVAIAVGLGARDHIANLIGAH
                     HLRQVFSVGQTVRVAGHQGRILEVTTEALVLDTVEGRVTLPGRVYSEQPITVITHRTD
                     GER"
     gene            2048..2896
                     /locus_tag="VD791_01000"
     CDS             2048..2896
                     /locus_tag="VD791_01000"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXV06580.1"
                     /translation="MASAETLTVAFLEAHPARAARVLERLPGPEAAALFARVPARVGG
                     PVLAAMPASTAARILSGLDDATTLALLTAVGAQAAVAVLRHAAEPRRTRLIEGLPTAT
                     AVASRLLLGSLEDSVGGWVDPDVIVLTPDSSVGEALARVRRGSEAEVEQLYVVDAEQR
                     LLGLVDLQAVVRAPEQTAVTALMREPAAALAAAAPLASAAAHEGWEGAAVLPVLERGE
                     RLLGVLRRSTLHRALAQAERPLPEAGETTIVGLLANGYWDAVSGLAEAALTLLPRLKP
                     ILPEER"
     gene            2893..4206
                     /locus_tag="VD791_01005"
     CDS             2893..4206
                     /locus_tag="VD791_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HXV06581.1"
                     /translation="MSIDALPAVTALNQRYLLDFPHEAARRLETMAAQEIGELLAAQP
                     PHAVVRAWEALAPDVARAVLESVPEALGRYLLTEAEPAASAGVLAQFEPADRERVLAA
                     VDAQVASELRELLQYPENSAGRLMDPNVSPLRTGMSVAEALARLRALKRGGLRELFVV
                     DQEGRLAGRVEIHDLAVADPARPLSEITRTLLAAVQDLDPREDVVSVLQQHPITELPV
                     VNHDGRFVGVIRQAALMSAVEQETSADIQTMVGASADERALSSPGFAVRKRLAWLQIN
                     LLTAFLAAAVVGLFESTIARFTALAVLLPVVAGQSGNAGAQALAVTMRGLVLREISLR
                     HWPRMVWKEAAVGLTNGLAVAATTALGVYVWSRSAGLVAVISSAMVISMVIAGVAGAL
                     VPIVLRRLGQDPAQSSSIVLTTVTDVAGFFSFLGIATLLSGLLAT"
     gene            complement(4254..4682)
                     /locus_tag="VD791_01010"
     CDS             complement(4254..4682)
                     /locus_tag="VD791_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV06582.1"
                     /translation="MSNKKQKFSASYAKDAVYRTGLRDFMEYRDLGIEEATHGRFRAH
                     VIRVKEDHRGGDQDLHTTGLHQHLCDFQMFYVLKGWIKFVYEGQKAERIFRAGDCVLQ
                     PPGIVHNEIACSDDVEVLEIYSPAVHETRPVAKMPEAAVQ"
     gene            complement(4798..5778)
                     /locus_tag="VD791_01015"
     CDS             complement(4798..5778)
                     /locus_tag="VD791_01015"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXV06583.1"
                     /translation="MKHVAGYLRRAIAALAIALAGGEAPAQSPPERPLKILVGYAPGG
                     PADALARIGAQILSEGLGQRVIVENRPGAGGSIAAGIVANAPPDGSVLLLTATADVIN
                     SLINREARYNIETSFTPIALIASAPNVLVVHPSVPVGSVKELIEYARARPGALNYGSA
                     GFGTVSHLAGAALAQGAGLEVVHVPYKGTAAAQVDLISGRLQFMFDAIGGALPNAKAG
                     RVKALAVTSPGRWPSAPELPTMAESGFPGFDMTAWFGLLAPAGSPAQSVARISEVVLK
                     GVQTVEARKRIGAIGGEPGQMTPAEFARYIRAENARWRKLFGDGRLSVEQ"
     gene            complement(5797..6522)
                     /locus_tag="VD791_01020"
     CDS             complement(5797..6522)
                     /locus_tag="VD791_01020"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HXV06584.1"
                     /translation="MADKKLTGKIAPERIRLMQVPRPPEGVIEGYRALGQASGILSDI
                     MDELGITGVIAASVLKPTLPGSCIVGPALTVRNMIQREHVYETARRHVNKMAEFEGHN
                     LACPGDVIVIDGVPGISNMGGISAQTGKRQGEAGAIVFGGVRDIAHSRSLGYPVWSTE
                     VTPLTGKWRIETVEINGEIEVAGVRVAPGDIVFADDTGVCFIPRVRAAEVLELARKKA
                     AAEAAKCQAIDAGTPVADLPSNA"
BASE COUNT          916 a         2263 c         2371 g         1116 t
ORIGIN      
        1 tgcgtagcag acgatctggc cgtggacgat ctgcgcaggc tgcgacacgc ttgtcgctca
       61 ggatgaagcc ctctggatcc tccgccggtg ggggaacagc tagggtagcg caggacatcc
      121 gcagcctcaa ggacccgaaa gcgccgctcg ccgggccgaa ccgccgcggg caaggtcgag
      181 cggggaccag cgacagcgcc tggccatgcg ggggagtacg ggttccgcgc ggtgacggca
      241 caatgtgagc tgcctctcat cttttcgacc gttcgcgacc gatgccctac gatcgcccgc
      301 ccctcaagat cggcctgtcc ccgcgctttc tgtacaagag tcccctggag ctgggtttcc
      361 gcggcaagac gctgctgtac ctggaacaat cgatcgccca ttgggtcatg tcgcacgggg
      421 ccctcatctt catgattccc tcgatcgaga ccggcggcct gatccggcgt ggcaacctca
      481 gcgtggaaga ctatgttgcg gcgctggacg gtctggtgct gcaagggggc accgatgtct
      541 cgccgcgcag ctacggacac gagccgctgc gcccggaatg gcagggcgac atcgtgcgcg
      601 accagtacga aatggagctg ctcgccgggt tcgtggacaa aggcaagccg gtgctgggta
      661 tctgccgcgg cgcgcaattg atcaacgtgg cctttggcgg cacgctgtgg caggacatcc
      721 cgagcatggt gccggcagcg ctgcggcatc acgacaccga gctctacgac gagaatttcc
      781 atccgctgcg gttcgagccg ggctcgctgc tggagaagat cttccccggt cagcgctccg
      841 gtctggtcaa ctccatccac catcaagcgg cgcgcgacct gggcgccggc ctgcaagtcg
      901 aaggccgggg cgacgccgac ggggtcgtcg aggcgatccg ctgggacgga ccgagcttcg
      961 tgtacggcat gcaatggcac ccggagttcc acccgggccg cgaggggctg ctcgactgca
     1021 caccgattct ggaggcgttc ctgcgcgagg cgaggcagcg catgcgcgga tgatggtcgc
     1081 gggctgccgc caccaccatg gcgcggcatc gtcgaccggg gcgcgatcga cggcaagccc
     1141 tggttgccgg gcatggcggg atgctaagct cggcctgcgc cgggttgccc gcggcgcgcc
     1201 cttcccgtcg gagcaggccc gatgaaccag ttgcgcagcc tcggcgacgc gctcgccatc
     1261 gtcggctcga ctcttctcga gcgctttgtc gtgttcgtcc ccagcgtgat cggcgcggtg
     1321 ctgctgctgc tggcgggctg ggtccttgcc cgcctactgc gggtgctggc gacgcgcgcg
     1381 gcgatgctgc tcgacacgct gctggcgcgc gtgggcgggc cggccggcgc agagcgcctg
     1441 cgcatgggtc gcgcggccgg aatgctcggc accggggtgt tctgggtggt gttgctgttt
     1501 ttcgccaccg ctgcgacgca ggtcctcggg ctgcagactt tcaccgactg gctggcgcgc
     1561 ctgatcgact acctgccgac gctggccgcc ggggtgctga tcgtggtcgt cggctacgtc
     1621 gtctcgcgcg tcgtgggaga tgtggtgcgc gcgaccgcta cgcgcctgga tgccgcgcag
     1681 cgctcggtgc tcgcccgcct gacccaggtc gcgatcctgg tcggcgcggt gctggtcggt
     1741 gccgatcaga tcggcatccg gatcaccttc ctcgccatct tcgccggcgc ggccgccgcg
     1801 gcggtcgtgg gcggggtggc gatcgcggtg gggctgggcg cgcgcgacca catcgcgaac
     1861 ctgattggcg cgcaccatct gcgccaggtt ttctcggtgg gtcagacggt gcgcgtggcc
     1921 ggacaccagg gacgcatcct cgaggtcacg accgaggcgc tcgttctcga taccgtcgaa
     1981 ggccgcgtga cgctgccagg gcgcgtctac agcgagcagc cgatcaccgt catcacacat
     2041 cgaaccgatg gcgagcgctg agactctgac cgtcgccttc ctcgaggcgc acccggcgcg
     2101 tgctgcacgc gtgctcgagc ggctgccggg acccgaggcg gcggcgctgt tcgcgcgcgt
     2161 gccggcgcgg gtcggcggcc ccgttctcgc cgcgatgccg gcgtccaccg ccgcgcgcat
     2221 cctcagcggt ctggacgacg cgaccacgct cgcgctgctg acggcggtcg gtgcgcaggc
     2281 ggcggtcgcg gtgctgcggc atgcggccga gccccggcgc acacggctga tcgagggcct
     2341 gcccacggcc actgcggtgg cctcgcgcct gctgctcggt tccctggagg actcggtggg
     2401 cggttgggtc gacccggatg tcatcgttct gacgccggac agctccgtcg gtgaggcgct
     2461 ggcccgcgtg cgccgcggga gcgaggcgga agtcgagcag ctctacgtgg tcgatgccga
     2521 gcagcggctg cttggactcg tcgacctgca ggccgtggtg cgcgcgccgg agcaaacagc
     2581 cgtgaccgcg ttgatgcgcg agccggccgc ggcgctggcg gcggccgcac cgctcgcaag
     2641 cgccgctgct cacgagggct gggagggggc tgccgtgctg ccggtgctgg agcgcggcga
     2701 gcgtctgctc ggggtgctgc gccggtccac gctgcatcgt gcgctcgccc aggccgagcg
     2761 cccgctgccc gaggccggcg agaccacgat cgtaggcctg ctcgcgaacg gctactggga
     2821 tgccgtgtcc gggttggcag aagcggcact gacgctgctt ccgcgcctca agccgatcct
     2881 gccggaggag cgatgagcat cgacgcgctg cctgccgtga ccgcgctgaa ccagcgctat
     2941 cttctcgatt ttccgcacga agccgcgcgc cgcctggaga cgatggcggc gcaggagatc
     3001 ggcgagctgc tcgccgcgca acccccgcat gcggtggtgc gcgcctggga agccctcgcg
     3061 ccggacgtag cgcgagcggt gctggaaagc gtgcccgagg cgctggggcg ctatctgctg
     3121 acggaggccg agcccgccgc cagcgcaggg gtgctggccc agttcgagcc tgccgaccgg
     3181 gaacgcgtgc tggcagccgt ggacgcgcag gtcgccagcg agctgcgcga gctgctgcaa
     3241 tatcccgaga actccgccgg ccgcctcatg gatcccaatg tcagcccgct gcgcaccggc
     3301 atgtcggtgg cggaagcact cgcccgcctg cgtgcgctca agcgcggagg gctgcgcgag
     3361 ctgttcgtgg tggaccagga aggccgtctc gccggccggg tcgagatcca cgacctcgcc
     3421 gtggccgatc cggcaaggcc gttgagcgag atcacgcgca cgctgctggc cgcggtgcag
     3481 gacctcgatc cccgcgagga cgtcgtgtcc gtgctgcagc agcatcccat cacggagctg
     3541 ccggtggtga accacgacgg gcgtttcgtg ggcgtcatcc ggcaggcggc gctcatgtcc
     3601 gcggtggagc aggagaccag cgccgacatc cagaccatgg tgggcgccag cgccgacgag
     3661 cgcgcgctgt ccagcccggg ctttgcggtg cgcaagcgcc tggcatggct gcagatcaac
     3721 ctgctcacgg cgttcctggc cgccgctgtc gtggggctgt tcgagagcac catcgccagg
     3781 ttcaccgcgc tcgccgtgct cctgccggtg gtggcagggc agtcgggcaa tgccggcgcg
     3841 caggccctgg cggtgaccat gcgcggcctg gtgctgcgcg agatcagcct gcgccactgg
     3901 ccgcgcatgg tgtggaagga ggcggcggtc gggctcacca acggactcgc ggtagccgcc
     3961 accacggcgt tgggcgtcta cgtctggagc cggtcggccg ggctggtggc ggtcatctcc
     4021 agcgcgatgg tgatctccat ggtgatcgcg ggcgtggccg gagcgctggt gcccatcgtc
     4081 ctgcgccgcc tgggtcagga cccggcgcag tcctcttcca tcgtgctcac caccgtcacc
     4141 gacgtggcgg gattcttctc tttcctcggg atcgcgacgc tgctgtcggg actgctcgcg
     4201 acgtagccgg ctgctgcgca accttcggcg agccaccgtc gcggttcggg cggctactgc
     4261 acggccgctt ccggcatctt ggcgaccggg cgcgtctcgt ggacggccgg cgagtagatc
     4321 tccagcactt cgacgtcgtc ggaacaggcg atctcgttgt ggacgatgcc cggcggttgc
     4381 agcacgcagt cgccggcgcg gaagatgcgc tctgccttct ggccttcgta gacgaacttg
     4441 atccagccct tgagcacgta gaacatctgg aagtcgcaca gatgctggtg caggccggtg
     4501 gtgtgcaggt cctggtcgcc gcctctgtgg tcctccttca cccggatcac gtgggcgcga
     4561 aagcggccgt gggtggcctc ctcgatgcct agatcgcggt attccatgaa gtcgcgcagg
     4621 ccggttcggt aaacggcgtc cttggcgtaa ctcgcggaga acttctgctt cttgttgctc
     4681 atggtgggcc cctctggatc gtcagccggt ggcgaaaggc ctagcgtagc gcaggacccc
     4741 tccaagctca aggacgccaa ggcgcggcgg cgcggctcga ctgccgcggc gcccgcatca
     4801 ttgctcgacg ctcagccgtc cgtcgccgaa gagctttctc cagcgggcgt tctcggcgcg
     4861 aatgtatcga gcgaactcgg ccggcgtcat ctgacccggc tcgccgccga tcgcgccgat
     4921 ccgcttgcgt gcctcgacgg tttgcacgcc cttcagcacg acttcggaga tgcgcgcaac
     4981 gctctgcgcg ggcgatcccg ccggggcaag caggccgaac caggcggtca tgtcgaatcc
     5041 ggggaagccg ctctcggcca tggtggggag ctcgggtgcg ctcggccagc gccccggtga
     5101 ggtgacggca agcgccttga ccctgccggc cttggcgttg ggcagcgcac cgccgatggc
     5161 gtcgaacatg aactgcagcc tgccgctgat gaggtcgacc tgggccgctg ccgtcccctt
     5221 gtagggtacg tgcaccacct ccagccccgc gccttgcgca agcgccgccc cggccaggtg
     5281 gctgaccgtg ccgaagccgg cggagccgta gttgagcgcc ccgggacggg ctctggcgta
     5341 ttcgatcagc tccttcaccg atccaaccgg aacggacggg tgcaccacca gcacgttggg
     5401 cgcgctggcg atcagcgcaa tcggcgtgaa gctggtctcg atgttgtacc gggcctcgcg
     5461 attgatgagc gagttgatga cgtcggcggt ggcggtcagc agcaggacac tgccgtcagg
     5521 gggcgcgttg gcgacgatgc ctgcggcgat gctgccgcct gctccggggc ggttctccac
     5581 gatcacgcgc tgacccaggc cctccgagag gatctgtgca ccgatgcgtg ccagcgcatc
     5641 ggccggtccg cccggcgcgt agccgaccag gattttcagc gggcgctctg ggggcgattg
     5701 cgcgggcgcc tcgccgcccg cgagcgcgat cgcaagagcg gctatcgcac gacggagata
     5761 gccggcaacg tgtttcaagt tacaactcgc acccgatcaa gcattgctcg gcagatcggc
     5821 cacgggggtg cccgcatcga tcgcctggca cttggccgcc tcggctgccg ccttcttgcg
     5881 ggccagctcc agcacttccg ctgcgcgcac gcgagggatg aagcaaacgc cggtgtcgtc
     5941 ggcgaacact atgtcgcctg gcgcgacgcg cacgcccgcc acttcgatct cgccgttgat
     6001 ctcgaccgtc tcgatgcgcc atttcccggt gagcggcgtc acctcggtcg accacaccgg
     6061 gtagcccagg ctgcgcgaat gcgcgatgtc gcgcacgccg ccgaacacga tggcacctgc
     6121 ttcgccctgg cgtttgccgg tttgcgcgga gatcccgccc atgttcgaga ttcccggcac
     6181 gccgtcgatc acgatcacgt cgcccgggca agccaggttg tgtccctcga actcggccat
     6241 cttgttcacg tgccggcgag cggtttcgta gacgtgctcg cgctggatca tgttgcgcac
     6301 cgtgagcgcc gggcccacga tgcacgagcc cggcagggtc ggcttcagaa ccgaggctgc
     6361 gatcaccccg gtgattccca gctcgtccat gatgtcggac aggatgccgc tcgcctgccc
     6421 gagcgcccgg tatccttcga tgactccctc gggagggcgc ggcacctgca tcaatcggat
     6481 gcgctcgggt gcgatcttcc cggtcagctt cttgtccgcc acggcaagct cctcgagtta
     6541 tctgcgaatg gtacagggcg ggtcggtcgg ctgctgactt cgcgtcgggg catcgtccgc
     6601 atgcaggtag aatcgccggt atgcgccgtg ttccgtcaga tcggcgccaa cgcaaggtgc
     6661 atgggt
//
LOCUS       DASPGE010000033        12472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_829920, whole genome shotgun sequence.
ACCESSION   DASPGE010000033 DASPGE010000000
VERSION     DASPGE010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12472
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829920"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /gene="argH"
                     /locus_tag="VD791_01025"
     CDS             <1..1109
                     /gene="argH"
                     /locus_tag="VD791_01025"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HXV06585.1"
                     /translation="RLTDLVGEAGKRLHTGRSRNDQVATDVRLWLRASIDAIDALMRA
                     LQSALVELAAQHVDTVMPGFTHLQVAQPVSFGHHLLAYYEMLKRDRARLADCRTRVNR
                     LPLGAAALAGTGWPIDRDRVARELGFDGVCANSLDAVSDRDFAIEFCACAALAMTHLS
                     RLSEELILWMNPRFGFVTLADSYCTGSSIMPQKKNPDVPELARGKTGRMNGHLMALLT
                     LMKGQPLAYNKDNQEDKEPLFDAVDTLSDTLRVFAGMLSGLTVDAAAMRRAAAEGFAT
                     ATDLADYLVRKGLPFRDAHEAVAQAVRFAAGEGKDLAQLPLDALRRFSPLIAEDVYAG
                     LTLEGALAARKHVGGTAPQQVRAAVARARAELAG"
     gene            1106..2011
                     /locus_tag="VD791_01030"
     CDS             1106..2011
                     /locus_tag="VD791_01030"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HXV06586.1"
                     /translation="MTPELVGVADWTAVAASIAQASGLPFTVEACAPVGGGCINSGYR
                     VRGAGRSYFVKLNSAQRAAMFEAELEGLAAIAATGTLRVPRPLCVGRDADHSWLVLEH
                     LELARADGSAMAALGERLAALHRCSAARFGWHRDNTIGATPQRNQWCADWVEFWRERR
                     LAFQLQLAADNGHRGALQERGERLLQHVQDFFARDRPAPSLLHGDLWAGNAACIAGGE
                     PVVFDPACYYGDREADLAMTELFGGFAPEFHAAYRNAWPLPDAYALRRDLYNLYHVLN
                     HLNLFGGGYGAQAERMIERLLSEVR"
     gene            2012..2782
                     /locus_tag="VD791_01035"
     CDS             2012..2782
                     /locus_tag="VD791_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV06587.1"
                     /translation="MLPLPELFSLSGKVALVTGASSGIGRTMVATLAEAGAAVVLLAR
                     DSARLEDAVRQIVSAGGRARALRCDLADRTRLDGAADQAAAAFGAPDILVNAAGINPR
                     PPVENISREMWDAVMAVNVDAAFFLAQRLAPAMRARRWGRIINLSSQQAIRAFNNSGA
                     YGVSKGAVSAMTRGLAEAWSRDGVTCNAIAPGYVPTGMTTGVFDGGERERWAAGLTLI
                     GRNGSLEDLRGITLFLASRASDYVTGQTIFVDGGMSAH"
     gene            2923..4260
                     /locus_tag="VD791_01040"
     CDS             2923..4260
                     /locus_tag="VD791_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV06588.1"
                     /translation="MGLFLAAALASGVARAQEPVRIGFVTFLSGPAAGPFGVPARHAA
                     EAIAESLNAGKGPAPHNARGFGGRRVELVVIDEAGGTTKQVSEFRNLVERQNVGYVVG
                     YISSGDCLAIAPVAEEMKKITVLFDCGTPRIFEEASYKYVFRTRPHATMDAVAAALYL
                     LETWPGVKSYAGINQNYAGIDHNYAGGQDSWNDFTMAMGVLKPEARVTTSQMPKFGAG
                     QYGAETSALMQSGPDVIHSSLWGGDLEAFVMQAGPRGLFKKSPLLLVAGEPYLHRLGS
                     AVPDGTIVGARGTNGVFAPDGPLNTWLRDIYRAKAGHDPTYPAYSAANAILGLKAAYE
                     KAKVAKLQRAIPMGANRGVKEETEKAYAEGPSDAELIAAFEGLIFDSPSGRMSMSLGK
                     GHQAVSGTAYGSTRLVGGKVTVIDIKRYPLEMVQPPEGVASADWIKGGMKPAK"
     gene            4278..5174
                     /locus_tag="VD791_01045"
     CDS             4278..5174
                     /locus_tag="VD791_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV06589.1"
                     /translation="MNQIPAILIDGIIYSSWLFVIAVGLTLIYGVMKILNVAHGSLYA
                     LGAYAATAFGGYWIAHGYPPLGSYGMLVVAAIAVGLTAGPLIERGLLRLMYGKEEVVL
                     VLVTYAVFLILEDLTKLAFGVDPLSIPEPYGLLGSIEVGDLIYPTYSLLVVLLSVVVG
                     IGLAMTLNRTRHGKILQAVIHDREMAAATGINVGRVYLVTFTVGTALAALGGALTAPT
                     ISVQPGIAAEVIVLAFAVVVIGGLGSLGGAALGAVIVGMVRAWAVHKWPEVELFSVYA
                     VMAAVLVMRPKGLFAVPEARKI"
     gene            5174..6115
                     /locus_tag="VD791_01050"
     CDS             5174..6115
                     /locus_tag="VD791_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV06590.1"
                     /translation="MLRGRTTPILLTALVVLLGAGPFLPRWAMFMLNVSLSWGTVVLG
                     MMLLMRTGLVSFGQGLYYCLGAYAAGVLYAKLGIGDIAVMLLASVAVTVAVSAVLGLL
                     LAKYRDIFFAMLSLAFSMILFGLLVKTSELGSTDGFGLSVASVAGVPLAEGWDRYAVF
                     ASAVVVAFGAALGIQRYLASHTGRLAGAIRDNELRVEYMGASVRRVIHLNYVISAALA
                     GLGGGLVGITIGHVDPEMAFWTTSGEFVFIAILSGTGNVLAPFLGATLFETVRSFAYE
                     YSPNTWQLVLGVTMLLVIMFLPGGLWSVFTRRKATRG"
     gene            6112..6906
                     /locus_tag="VD791_01055"
     CDS             6112..6906
                     /locus_tag="VD791_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV06591.1"
                     /translation="MSVILEARRLNKSFGAVTAAADISVGVERDSVVGLIGSNGAGKT
                     TFINMVTGYLAPTSGSIHYGDRDITGMAPREITRLGICRSFQIPQLFDNLSVRENLQV
                     GVGIAARSARAAVDTQGEAGGHPRGEAGEIDTLVERFNLGGYRDQPAGLLPEGVRKLL
                     DIAMAVAGHPNLLLLDEPTSGVSADEKFRLMDMVMGALQAQGVTVLFVEHDMEVVARY
                     THRILAFYEGRIIADGEPQAVLRDADVRRHVIGEDLHPGRAEAARG"
     gene            6899..7549
                     /locus_tag="VD791_01060"
     CDS             6899..7549
                     /locus_tag="VD791_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV06592.1"
                     /translation="MVKLEGLDVAIGAVSILRNVNLELPSGAFTGLIGRNGAGKTTLL
                     RSIMGILEARNGSIHFDGATLDTMPTHLRARAGIGYMPEDRRLVPNLTVEENVLMPAW
                     AAGLPDVSARLARVYEMIPEVEAFAGRKAMQLSGGQQKLAALARALMCGSRVLLLDEP
                     FEGVAPVLARRLAEVIADLKQEGLSILLTESDLSHSREMLDSLYVIDRGEVSPGQL"
     gene            complement(7631..8776)
                     /locus_tag="VD791_01065"
     CDS             complement(7631..8776)
                     /locus_tag="VD791_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXV06593.1"
                     /translation="MERVQLHRFIDLAKPVEPRGEPRAMSRRQFLKAASAVAAVTGMS
                     SDAFARNFIDQYDADGPIARYPNPDVIVLDKRFKYKLGNTPIVRLYRGTMWAEGCAWN
                     GVGRYLVWSDIPNNEQLRWCEEDGHVGRRFHYPSGNSNGNTFDYQGRQISFEHGTRRV
                     GRYELNGSYTVLADKSPDGKGLNAPNDGMAHPEGWVLFTDPGYGGLMNYEGERLNTGS
                     PQPIQKEAVYRIDAPGKIEKVTDEPFKPNGLCFSPDFKKVYIADTGLSHYPNAKSIIW
                     VYDVDGKRLKNGKTFANMEMNGKIGFADGIRCDEDGNVWAGMGWVGDGYDGVHVFSPD
                     GTRIGQIRMPEITGNITFGGTKRNRLFMAASTSLYAVYVETRGGTLT"
     gene            8756..9529
                     /locus_tag="VD791_01070"
     CDS             8756..9529
                     /locus_tag="VD791_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06594.1"
                     /translation="MKLYAFHAGVSSRCYCARPAALHRGTRGRNDSPPVLLLLHPRAA
                     CNRGECRARRAPAQRFAYHARRPKKAWSSIMRLRAFFALLLLAGSTNAAEPGPAGAAK
                     SDAATGGCDAYQWDMNREMFLLGTAPIAVAALKARAEDARFTPLDRRLQVTLHPAAEV
                     ALLAPPGRAHDAATNHAGLLLLYVPRSGAYRISSDQRLWIDVVGPDGVVKSSRFAMQT
                     GCAKLLKSVAFRLDPEIGYWIQLTGSPAREPMLLVTLDR"
     gene            9573..10742
                     /locus_tag="VD791_01075"
     CDS             9573..10742
                     /locus_tag="VD791_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HXV06595.1"
                     /translation="MTSKPSQQAFATRAIHAGQEPDPTTGAVMPPIYATSTFKQRSPG
                     EHTGFEYARTQNPTRMAFERCVANLESGRAAWAFSSGLAAMGTVLELLDAGSHMVAVD
                     DIYGGSWRLFERVRGRSMGLRVTYVDPDRPQSLEPAIRADTRMIWVETPSNPLLKVTD
                     LAAVARAAKARGILTVCDNTFASPYLQRPLEHGFDIVVHSVTKYINGHSDAVGGVVAA
                     GDNAELIERFGFMQNAIGSIMDPFTSFLALRGAKTLPLRMDRHCANALELAHWLDRHP
                     KTEKVIYPGLPKHPQHALAAAQMRGFGGMIAVYLRTDLAGTKRFLEGCRLFTLAESLG
                     GVESLAGHPVLMSHASLPAERRALLALTDNLVRLSVGIEDARDLIADLEQALERV"
     gene            10760..11269
                     /locus_tag="VD791_01080"
     CDS             10760..11269
                     /locus_tag="VD791_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HXV06596.1"
                     /translation="MSSRLVFLAVCLACAGLMGFGLYLQHAQGLEPCPLCILQRYAFV
                     ATGLVALIAAVHGPARVGSAVYGLLILLAAGAGGTVAGRQVWLQHNPPQVLDCGPDLA
                     YMLDSFPLSQVLPKVFRGEGDCAKVVWKFLGLSIPEWALLWFVLFAAAGLYVLLVRPA
                     RAARPFVAG"
     gene            complement(11278..11913)
                     /gene="nthA"
                     /locus_tag="VD791_01085"
     CDS             complement(11278..11913)
                     /gene="nthA"
                     /locus_tag="VD791_01085"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HXV06597.1"
                     /translation="MHVHSDHDHPHPPDSDDALTYYQAMEIAVRELLIEKGVLTADEL
                     RRQVEDMDNRTPARGAQVVARAWVDPAYKARLLADGNSALEEIGLDRGPYKLVVVENT
                     PAVSDAAPVHNVIVCTLCSCYPRWLLGLPPDWYKSRAYRSRVVREPRAVLREFGLELP
                     GEVVLRVHDSTADMRYLVLPARPAGTDGWSEERLAAIVTRDCMIGVAIPRT"
     gene            complement(11924..12232)
                     /locus_tag="VD791_01090"
     CDS             complement(11924..12232)
                     /locus_tag="VD791_01090"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HXV06598.1"
                     /translation="MSTPRIDPIAQRFQPGDSVRVRRADPPGHVRTPWYIRGQTGVVE
                     RVCGCYPNPEELAYARPGLPAQPLYRVRFLQREVWPGYTGGARDTVDIEIYQHWLAPA
                     "
     gene            complement(12229..>12472)
                     /locus_tag="VD791_01095"
     CDS             complement(12229..>12472)
                     /locus_tag="VD791_01095"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06599.1"
                     /translation="FWETRVAALLVLLTRKQRMNVDELRRNVEILGADAYDKLSYYER
                     WIHSIAQTLIQRGVISVDELGRKMAEIERRPRDPGP"
BASE COUNT         1821 a         4293 c         4328 g         2030 t
ORIGIN      
        1 agcgcctcac cgacctggtc ggtgaggccg gcaagcgcct gcacaccggc cgctcgcgca
       61 acgatcaggt ggcgaccgac gtgcgcctgt ggctgcgcgc gtcgatcgac gcgatcgacg
      121 ccctgatgcg cgcactgcaa agcgcgctgg tggagctcgc cgcgcagcat gtcgacacgg
      181 tgatgccggg attcacgcac ctgcaggtcg cgcagccggt cagcttcggc catcacctgc
      241 tcgcctatta cgaaatgctc aaacgcgacc gcgcgcggct ggccgattgc cgcacgcgcg
      301 tcaatcgtct gcccctgggc gcggcggcac tggccggcac cggctggccg atcgaccgcg
      361 accgggtcgc gcgcgagttg gggttcgacg gcgtgtgcgc caactcgctg gacgcggtgt
      421 ccgatcgcga cttcgccatc gaattctgcg cctgcgcggc gctcgccatg acccatctgt
      481 cgcgcttgtc ggaagagctg atcctgtgga tgaacccgcg tttcggcttc gtcacgctgg
      541 ccgacagcta ttgcaccggc tcgtcgatca tgccgcagaa gaagaaccct gacgtgcccg
      601 agctggcacg cggcaagacc gggcgcatga acggacacct gatggcgctg ctcacgctga
      661 tgaagggcca gccgctcgcc tacaacaagg acaatcagga ggacaaggag ccgctgttcg
      721 atgcggtgga caccctcagc gacacgctgc gcgtgttcgc ggggatgctg tcgggcttga
      781 ccgtcgacgc cgcggcgatg cgccgcgccg cggccgaagg tttcgccacc gccaccgatc
      841 tcgccgacta cctggtgcga aagggcctgc cgtttcgcga cgcgcacgaa gccgtggcgc
      901 aagcggtgcg cttcgctgcg ggcgagggca aagacctcgc gcagttgccg ctcgatgcgc
      961 tgcgccgttt ctcaccgctg atcgccgagg acgtgtatgc cgggctcacg ctggagggcg
     1021 cgctggccgc ccgcaagcac gtcggtggca ccgcaccgca gcaggtgcgc gccgccgtcg
     1081 cccgtgcgcg cgccgagctg gccggttgac ccccgagctg gtgggcgtcg ccgactggac
     1141 cgcggtcgcg gcctcgatcg cgcaggcgag cggcctgccg ttcaccgtcg aagcctgcgc
     1201 accggtcggc ggcggctgta tcaacagcgg ctaccgcgtg cgcggggcgg gccgcagcta
     1261 cttcgtcaag ctcaacagtg cgcaacgcgc ggcgatgttc gaggcggagc tggagggcct
     1321 cgccgccatc gccgccaccg gcaccttgcg ggtcccgcgg ccgttgtgcg tggggcgcga
     1381 tgccgaccac agctggctgg tgctcgagca tctcgagctg gcgcgcgcag acggctcggc
     1441 aatggcggcg ctgggggagc ggctcgcggc gctgcaccgc tgcagcgcgg cacgcttcgg
     1501 ctggcaccgc gacaacacca tcggcgcgac gccacagcgc aaccagtggt gtgcggactg
     1561 ggtcgagttc tggcgcgagc ggcggctggc gtttcagttg cagctggcgg cggataacgg
     1621 ccaccgcggg gcgctgcagg agcgcggcga gcggctgctg cagcacgtgc aggatttttt
     1681 cgcccgcgac cggccggcgc cgtccctgct gcacggcgat ctgtgggccg gcaacgcggc
     1741 ttgcatcgcc ggcggggagc cggtggtgtt cgacccggcc tgctactacg gcgaccgcga
     1801 agcggacctg gcgatgaccg agctgttcgg cggcttcgcg cccgaattcc acgccgcgta
     1861 tcgcaacgcc tggccgctgc cggacgctta tgcgctgcgg cgcgacctgt acaacctgta
     1921 tcacgtgctc aatcacctga acctgttcgg cggcggctat ggcgcgcagg ccgagcgcat
     1981 gatcgagcgt ttgctgagcg aggtgcgttg aatgctgccg ctgccggagc tgttctcgct
     2041 gtcgggcaag gtggcgctgg tgacgggcgc gagctcgggc atcggccgga cgatggtcgc
     2101 gacgctggcc gaagccggcg cggcggtggt gctgctggcg cgcgacagcg cgcgcctgga
     2161 ggacgcggtg cgtcagatcg tgagcgccgg cggccgcgct cgggcgctgc gctgcgacct
     2221 cgccgaccgc acacgactcg atggcgcagc ggaccaggcc gcggcagcgt tcggcgcgcc
     2281 cgacatcctg gtcaacgccg ccgggatcaa cccgcgcccg ccggtcgaga acatcagccg
     2341 cgagatgtgg gacgcggtga tggcggtgaa cgtagacgcg gccttcttcc tggcgcaacg
     2401 gctcgccccc gccatgcgcg cccggcgctg gggccgcatc atcaatctgt cgtcgcagca
     2461 ggcgatccgt gccttcaaca acagcggcgc ctacggggtt tcgaagggcg cagtctcggc
     2521 catgacgcgc gggctggccg aggcctggtc gcgcgacggt gtcacctgca acgctatcgc
     2581 cccgggctat gtgccgaccg gcatgaccac cggcgtgttc gacggcggcg agcgcgagcg
     2641 ctgggccgcc gggctcaccc tgatcgggcg caacggcagc ctggaagatc tgcgcggcat
     2701 cacgctgttc ctggcgagcc gagcgagcga ctacgtcacg ggccagacca tcttcgtgga
     2761 cggcggcatg tcggcccatt gagcccattg gggagcgtag gccggcgcgc ctttcggtcc
     2821 gctagaatgc ggggcgttcg cgtcccggcc aaactgctca agccgggcgc gtcatcacaa
     2881 gcgggggagg agatggcaca cgttggcgcg ccgcggtggc tcatgggcct gtttctagcc
     2941 gccgccctcg cgtccggagt cgcccgggcg caggagccgg tgcgcatcgg tttcgtcacc
     3001 ttcctgtccg gacccgcggc cggcccgttc ggcgtcccgg cgcggcacgc ggccgaggcc
     3061 atcgccgaat cgctcaacgc cggcaaaggc ccggccccgc acaacgcgcg cggattcggc
     3121 ggccgccggg tggagctggt ggtgatcgac gaggcgggcg gcaccaccaa gcaggtgagc
     3181 gagttccgca atctggtcga gcgccagaac gtgggctacg tcgtcggcta tatctccagc
     3241 ggcgactgcc tggccatcgc gccggtggcc gaggagatga agaagatcac ggtgctgttc
     3301 gattgcggca cgccgcggat cttcgaggag gccagctaca agtacgtgtt ccgcacccgc
     3361 ccccacgcca ccatggacgc cgtggccgcc gccctgtacc tgctcgagac ctggcccggc
     3421 gtgaagagct acgccggtat caatcagaac tacgccggca tcgatcacaa ctacgcgggg
     3481 ggccaggact cctggaacga cttcaccatg gccatgggcg tgctcaagcc cgaggccagg
     3541 gtcaccacct cgcagatgcc caagttcggg gccgggcaat acggcgccga gacctcggcg
     3601 ctgatgcaga gcggccccga cgtgatccat tccagccttt ggggcggcga cctcgaggcg
     3661 ttcgtcatgc aggcgggccc gcgcggactg ttcaagaaga gcccgctgct cctagtggcc
     3721 ggcgagccct atctgcaccg gctcggcagc gccgtccccg acggcaccat cgtcggcgcg
     3781 cgcggcacca acggcgtgtt cgcgcccgac ggcccgctca acacgtggct gcgcgacatc
     3841 taccgcgcga aggccggcca cgaccccacc tacccggcgt acagcgcggc caacgccatc
     3901 ctcggtctga aggccgcgta cgagaaggcc aaggtggcca agctgcagcg ggcaatcccg
     3961 atgggcgcca accggggcgt caaggaggaa accgaaaagg cctacgccga aggtccgagc
     4021 gacgcggagc tgattgccgc attcgaaggg ctgatcttcg actcgccgag cggcaggatg
     4081 tcgatgtcgc tgggtaaggg tcaccaggcc gtgtcgggca ccgcgtacgg cagcaccagg
     4141 ctggtcggcg gcaaggtgac ggtgatcgac atcaagcgct acccgctgga gatggtgcaa
     4201 ccgccggagg gcgtggccag cgcggactgg atcaagggcg gcatgaagcc cgcgaagtag
     4261 cgacccgctg ccgccgcatg aaccagatcc ccgccatcct gatcgacggc atcatctact
     4321 cgtcctggtt gttcgtcatc gccgtgggcc tcacgctgat ctacggcgtg atgaagatcc
     4381 tcaacgtcgc gcacggcagc ctgtacgccc tgggcgcgta cgccgccacc gcgttcggcg
     4441 gctactggat tgcgcacggc tatccgccgc tcggcagcta cggcatgctg gtcgtcgctg
     4501 cgatcgcggt cgggctcacc gccgggccgc tgatcgagcg cgggctgctg cgcctgatgt
     4561 acggcaagga agaagtggtg ctggtgctgg tcacctacgc cgtgttcctg attctcgagg
     4621 acctcaccaa gctcgccttc ggcgtggacc cgctgtcgat cccagagccc tacgggctgc
     4681 tgggcagcat cgaggtcggc gacctcatct accccaccta cagcctgctg gtggtgctgc
     4741 tgtcggtggt cgtgggcatc ggtctcgcga tgaccctgaa ccgcacgcgc cacggcaaga
     4801 tcctgcaggc ggtgatccac gaccgcgaga tggcggcggc caccggcatc aacgtcggcc
     4861 gcgtgtatct ggtcaccttc accgtcggca ccgcgctcgc cgcgctgggt ggcgcgctca
     4921 ccgcgcccac catctcggtg cagcccggca tcgccgccga agtgatcgtg ctcgccttcg
     4981 cggtggtggt gatcggcggc ctgggcagcc tgggcggggc ggcgctgggc gcggtgatcg
     5041 tgggcatggt gcgcgcctgg gcggtgcaca agtggccgga ggtggagttg ttcagcgtct
     5101 acgcggtgat ggcggcggtg ctcgtgatgc gccctaaggg cctcttcgcc gtgccggaag
     5161 cgaggaagat ctgatgctgc gcggccgcac cacgcccatt ctgctcaccg cgctggtggt
     5221 gctgctcggg gccggcccgt tcctgcccag atgggcgatg ttcatgctca acgtatcgct
     5281 gtcctggggt acggtggtgc tgggcatgat gctgctgatg cgcaccgggc tggtgtcctt
     5341 cggccagggc ctgtactact gcctcggcgc ctacgcggcc ggagtgctgt acgccaagct
     5401 cggcatcggc gacatcgccg tcatgctgct ggcgagcgtg gcggtgacgg tggcggtgtc
     5461 cgccgtgctg gggctgctgc tcgccaagta tcgcgacatc ttcttcgcca tgctgagcct
     5521 ggcgttctcg atgatcctgt tcggcctgct ggtgaagacc tccgagctcg gcagcaccga
     5581 cggcttcggc ctgagcgtgg cctcggtggc cggcgtgccg ctcgccgagg gctgggaccg
     5641 ctacgccgtg ttcgccagcg cggtggtggt ggcgttcggc gccgcgctgg gcatacagcg
     5701 ctacctcgcc tcccataccg gaaggctggc cggcgcgatc cgcgacaacg agctgcgggt
     5761 ggagtacatg ggcgcgtcgg tgcgccgcgt gatccatctc aattacgtga tcagcgccgc
     5821 gctcgccggg ctgggcggcg ggctcgtggg catcaccatc ggccacgtgg acccggaaat
     5881 ggcgttctgg accacctccg gcgagttcgt cttcatcgcc atcctgtcgg gtaccggcaa
     5941 tgtgctggcg ccgtttctcg gcgctaccct cttcgagacg gtgcgcagct tcgcctacga
     6001 atactcgccc aacacctggc agctggtgct gggcgtcacc atgctgctgg tgatcatgtt
     6061 cctgcccggc gggctatggt ccgtgttcac gcgccgaaag gccaccagag gatgagcgtc
     6121 atcctcgagg cgcgccgcct caacaagagc ttcggcgcgg tcaccgccgc cgccgacatc
     6181 agcgtcggcg tcgagcgcga cagcgtggtg ggcctgatcg gcagcaacgg cgccggcaag
     6241 accaccttca tcaacatggt gaccggctac ctcgcgccca cgtcgggcag catccactac
     6301 ggcgaccgcg acatcaccgg catggccccg cgcgagatca cgcggctcgg catctgccgc
     6361 tcgttccaga tcccgcagct gttcgacaac ctgagcgtgc gcgagaacct gcaagtggga
     6421 gtgggcatcg cggcgcgctc cgcgcgcgcc gccgttgaca cgcagggaga agcaggcggc
     6481 cacccgcgag gcgaggccgg cgagatcgac acgctggtcg aacgcttcaa cctgggcggc
     6541 tatcgtgacc agcctgccgg gctgctgccc gagggcgtgc gcaagctgct cgacatcgcc
     6601 atggccgtgg ccggccaccc caacctgctg ctgctggacg agcctaccag cggcgtatcc
     6661 gcggacgaga agttccgcct gatggacatg gtgatgggtg cgctccaggc gcagggcgtc
     6721 acggtgctgt tcgtcgagca cgacatggag gtcgtggcgc gctacacgca ccgcatcctg
     6781 gcgttctacg aaggccgcat catcgccgac ggcgaaccgc aggcggtgct gcgcgacgcc
     6841 gacgtgcgcc ggcacgtgat cggcgaggat ctgcaccccg gcagggcgga ggccgcccgt
     6901 ggttaagctc gaggggctgg acgtggccat cggtgcggtg agcatcctgc gcaacgtgaa
     6961 cctggagctg ccgtcgggtg cgttcaccgg gctgatcggg cgcaacggcg ccggcaagac
     7021 cacgttgctg cgctccatca tgggtattct cgaagcgcgc aacggctcga tccacttcga
     7081 cggggcaacg ctggacacga tgcccacgca cctccgcgcg cgcgcgggga tcggctacat
     7141 gcccgaggac cggcgcctgg tgccgaacct cacggtggag gagaacgtcc tgatgccggc
     7201 ctgggccgcc ggcctgccgg acgtgtcggc gcggctggcg cgcgtctacg agatgatccc
     7261 ggaggtcgag gccttcgccg gccgcaaagc gatgcagctc tccggcggcc agcagaagct
     7321 tgctgcgctg gcgcgcgcgc tcatgtgcgg gagcagggtg ctgctgctgg acgaaccgtt
     7381 cgagggagtg gcgccggtgc tcgcgcggcg cctcgccgag gtgatcgccg atctcaaaca
     7441 ggaaggcctg tctatcctgc tcaccgaatc ggatctgtcg cactcccgcg aaatgctcga
     7501 cagcctgtac gtgatcgacc gcggggaggt ctctccgggg cagctctaat tcaaccgcag
     7561 aggcgcaagg aacaccgttc ctgtattcgc ccgcgcctcg gcggcaagac cgtagcaggt
     7621 cacgcgggaa tcaggtcagc gtgccgccac gcgtctcgac atagaccgcg tacagggacg
     7681 tgctggccgc catgaatagg cggttgcgct tggtaccccc gaaggtgatg ttgccggtga
     7741 tctcgggcat gcggatctgg ccgatgcggg tgccgtcggg cgagaacacg tgcacgccgt
     7801 cgtagccgtc gcccacccag cccatgccgg cccagacgtt gccgtcttcg tcgcagcgaa
     7861 tgccgtcggc gaagccgatc ttgccgttca tctccatgtt ggcgaacgtc ttgccgttct
     7921 tcagccgctt gccgtccacg tcgtacaccc agatgatgct cttggcgttc gggtaatgcg
     7981 acagcccggt gtcggcgatg tacaccttct tgaagtcggg cgagaagcac agcccgttcg
     8041 gcttgaacgg ctcgtcggtc accttctcga tcttgcccgg cgcatcgatg cggtacacgg
     8101 cctccttctg gatcggctgc gggctgccgg tgttgagccg ctcgccctcg tagttcatca
     8161 gcccgccgta gcccgggtcg gtgaacagca cccagccttc cgggtgcgcc atgccgtcgt
     8221 tgggggcgtt cagtcccttg ccgtccgggc tcttgtccgc cagcacggtg tagctgccgt
     8281 tcagctcgta gcggcccacg cggcgcgtgc cgtgctcgaa ggagatctgg cggccctggt
     8341 agtcgaaggt gttgccgttg gaattgccgg acgggtagtg gaagcgccgg ccgacgtggc
     8401 cgtcttcctc gcaccagcgc agctgctcgt tgttggggat gtcgctccac accaggtagc
     8461 ggcccactcc gttccaggcg cagccctcgg cccacatggt gccgcggtac aggcgcacga
     8521 tcggggtgtt gccgagcttg tacttgaagc gcttgtccag cacgatgacg tcgggattgg
     8581 ggtagcgcgc gatcggcccg tccgcgtcgt actggtcgat gaagttgcgg gcaaaggcgt
     8641 ccgacgacat gccggtgacc gcggctaccg cagacgccgc cttcaggaac tggcgccggc
     8701 tcatcgcccg gggctctccc ctgggctcga ccggcttggc caaatcgatg aagcggtgaa
     8761 gctgtacgcg ttccatgctg gagtctcctc tcgatgttat tgcgcacgcc ccgcggcgct
     8821 gcaccgcggc acgcgcgggc ggaacgatag tccgccggtc ctccttctac tccatccgcg
     8881 cgcggcgtgc aaccggggcg aatgccgcgc gcggcgagcg cccgcgcagc ggttcgcgta
     8941 ccatgcacgc cgacccaaga aagcctggtc atcgatcatg cgactccgtg cgtttttcgc
     9001 gctgctgctg ttggccggtt ccacgaacgc cgccgagccc ggccctgctg gcgcagccaa
     9061 atccgacgcg gcgaccggcg gctgcgacgc ctaccaatgg gacatgaacc gcgagatgtt
     9121 cttgttgggc accgcgccga tcgcggtggc cgcgttgaaa gcgcgcgctg aggatgcgcg
     9181 cttcaccccg cttgaccggc gcctgcaggt gaccctgcac cccgcagccg aggtggcgct
     9241 cctcgcgccg ccgggccggg ctcacgacgc ggcgacgaac cacgcgggtc tgttgttgct
     9301 gtacgtgccg cgcagcggcg cctaccgcat ttcgtcggac cagcgcctgt ggatcgacgt
     9361 ggtcggcccc gacggcgtgg tgaaatcgag ccggttcgca atgcagaccg gctgcgccaa
     9421 gctgctgaag agcgtcgcct tccggctcga ccccgagatc gggtactgga tccagctcac
     9481 cggcagcccg gcgcgcgagc ccatgctcct cgtcacgctc gaccgctagc acgctacact
     9541 gggcgtacgc agcgatcctc cacggcacga ccatgaccag caagccctcg caacaggcat
     9601 ttgccacccg cgccatccac gccggccagg aacccgatcc gaccaccggc gcggtgatgc
     9661 cgccaatcta cgcgacttcg accttcaagc agcgctcgcc gggcgagcac accggcttcg
     9721 agtacgcgcg cacgcagaac cccacgcgca tggccttcga gcgctgcgtc gcgaacctgg
     9781 agtccggccg cgccgcctgg gcgttctcct ccggcctggc ggccatgggc acggtgctgg
     9841 agctgctcga tgccggcagc cacatggtgg cggtagacga catctacggc ggcagctggc
     9901 ggctgttcga gcgcgtgcgc gggcgctcca tgggcctgcg cgtgacctac gtcgacccgg
     9961 accggccgca atcgctggag ccggccatcc gcgccgacac gcgcatgatc tgggtggaga
    10021 cgccgagcaa tcccctgctc aaggtgaccg acctggccgc ggtggcgcgc gcagcgaaag
    10081 cgcgcgggat cctcaccgtg tgcgacaaca ccttcgcctc gccttatctg caacgcccgc
    10141 tggaacacgg tttcgatatc gtggtgcatt cggtcaccaa gtacatcaac ggccactccg
    10201 acgcggtcgg cggcgtggtg gcggcgggcg acaatgccga actgatcgag cgcttcggct
    10261 tcatgcagaa cgcgatcggc tcgatcatgg atccgttcac cagcttcctc gcgctgcgcg
    10321 gcgcgaagac cctgccgctg cgcatggatc ggcactgcgc caatgcgctg gagctggcgc
    10381 actggctcga ccgccacccg aagacggaga aggtgatcta tcccggcctg cccaagcacc
    10441 cgcagcatgc gctggcggct gcgcagatgc gcggcttcgg cggcatgatc gcggtgtacc
    10501 tgcgcaccga tctcgcggga accaagcgct tcctggaagg ctgccggctg ttcaccctgg
    10561 cggagagcct gggcggggtg gagagcctgg ctgggcaccc ggtcctgatg agccatgcgt
    10621 cgctccccgc ggagcgccgc gccctgctcg cgctcaccga caatctcgta cgcctgtcgg
    10681 tcggcatcga ggacgcgcgc gatctgatcg ccgacctcga gcaggcgctc gagcgggtct
    10741 gacgccgggt cgccgtccct tgtccagccg cctcgtgttc ctcgccgtct gcctggcctg
    10801 cgccgggttg atgggtttcg ggctctacct gcagcacgcg caaggcctgg agccctgccc
    10861 gctgtgcatc ctgcagcgct atgcgttcgt ggcgaccggg ctcgtcgctc tgatcgctgc
    10921 cgtgcacggg cctgcgcgtg tgggcagcgc tgtctacggg ctgctgattc tcctggccgc
    10981 cggcgccggc ggtacggtgg ccggccgcca ggtctggctg cagcacaacc cgccgcaagt
    11041 gctcgactgc ggtccggatc tggcgtacat gctggattcc ttcccgctgt cgcaggtgct
    11101 gcccaaggtg ttccggggcg agggtgactg cgccaaggtg gtgtggaagt tcctggggct
    11161 gtcgattccg gaatgggcgc tgctgtggtt cgtgctgttc gcggcagcag gcctgtacgt
    11221 cctgctcgtg cgtccggcgc gtgccgcgcg cccgttcgtg gccggctgag ctggaaatca
    11281 ggtcctcggg atcgccaccc cgatcatgca gtcgcgcgtg acgatggcgg cgagccgctc
    11341 ctcgctccag ccgtcggtgc cggccggacg ggccggcaga accaggtagc gcatgtccgc
    11401 agtggagtcg tgcacccgca gcaccacctc tcccggcagt tccagcccga actcgcgcag
    11461 cacggcgcgc ggctcgcgca ccacccggct gcggtaggcg cggctcttgt accagtcggg
    11521 cggcagaccc agcaaccagc gcggatagca cgagcacagc gtgcaaacga tgacgttgtg
    11581 caccggggcc gcgtcgctca cggccggcgt gttctccacc accaccagct tgtacgggcc
    11641 gcggtcgagg ccgatctcct ccagcgccga gttgccgtcc gccagcaggc gcgccttgta
    11701 agccgggtcg acccatgcgc gcgccaccac ttgggcgccg cgcgccgggg tgcggttgtc
    11761 catgtcctcg acctggcgcc gcagctcgtc ggcggtgagc acgcccttct cgatcagcag
    11821 ctcgcgcacc gcgatttcca tcgcctggta gtaggtcaac gcgtcgtcgc tgtcgggcgg
    11881 gtgtggatgg tcgtggtcgg aatgaacgtg catgcgtgcc tcgtcaagcg ggggccagcc
    11941 agtgctggta gatctcgatg tccacggtgt cccgcgcgcc gcccgtgtag cccggccaca
    12001 cttcgcgctg caggaaccgc actcggtaca ggggctgcgc gggcagtccc gggcgcgcat
    12061 aggcgagttc ttccgggttg ggatagcaac cgcagacgcg ttcgaccacg cctgtttgcc
    12121 cgcggatgta ccagggcgtg cgcacgtgac ccggcggatc cgctcgccgc acgcgcacgc
    12181 tgtcgcccgg ttgaaaccgc tgcgcgatcg ggtcgattcg cggcgtgctc acgggccagg
    12241 gtctcgaggg cgccgttcga tctccgccat cttgcgcccg agctcgtcca cgctgatgac
    12301 gccacgctgg atcagcgtct gcgcgatgga atgaatccag cgctcgtagt aggaaagctt
    12361 gtcgtaagcg tccgcgccca ggatctcgac gttccggcgc aactcgtcca cgttcatccg
    12421 ctgcttgcgc gtcagcagca ccagcagcgc ggcgacgcgc gtctcccaga ac
//
LOCUS       DASPGE010000034         6464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_361293, whole genome shotgun sequence.
ACCESSION   DASPGE010000034 DASPGE010000000
VERSION     DASPGE010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361293"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VD791_01100"
     CDS             complement(<1..255)
                     /locus_tag="VD791_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06600.1"
                     /translation="MQRAASFGLLCALAGAAAPSLPVRAEPALSPEQIIAALRKPSSS
                     TGNIADAVEEATGARGFMYHDMKPVFKAKIVGRAATALLRP"
     gene            377..565
                     /locus_tag="VD791_01105"
     CDS             377..565
                     /locus_tag="VD791_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06601.1"
                     /translation="MSSAEFSGIYPMQYAFFDRGGGLDRAAMRRQVEAALAGARCACS
                     TGAAGTSCRTTCAPGAPA"
     gene            502..849
                     /locus_tag="VD791_01110"
     CDS             502..849
                     /locus_tag="VD791_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06602.1"
                     /translation="MFNGRGGNELPDNLRAGCAGLIPATDTFDFQVRVFELMRSGRAQ
                     DEARAEAVYREVLPAIVFAMQSLDTLICYGKRLAALRLGLPEVHDRAPALALTASGLA
                     CVRRYAAALGFLV"
     gene            1012..1908
                     /locus_tag="VD791_01115"
     CDS             1012..1908
                     /locus_tag="VD791_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXV06603.1"
                     /translation="MGDEFLPSEAEAKVVNGWIALLIVLALLAFGVYFLAAGLPRSEG
                     GPLGTGLALVTVGLICLHGMFTLQPNEAAVMTFFGSYAGTERHAGFHWANPFCQRRRM
                     SLRAHNLTTPILKVNDGRGSPIEIGAVVVWRVQDTARACFEVEDYTEYVRLQCESGLR
                     EVASRHAYDNADETLLGGRVRTLRGDAERISETLRATIQARAGLAGVAVEEARIAHIA
                     YAPEIAPAMLRRQQAEAVIAARRKLVEGAVGMVEMALIDINERCLASFTPAQKVALVT
                     NLMTVLVGEGQPQPVVPMKQES"
     gene            complement(1933..2508)
                     /locus_tag="VD791_01120"
     CDS             complement(1933..2508)
                     /locus_tag="VD791_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXV06604.1"
                     /translation="MAYALPVHADIYRYVDQDGKVHFTNVPPDSRFKLYLATEKEPSA
                     VANTITHRYRPYSPADRKKYHAHVVAAARAFKLEPALIHAVISAESGYNPLARSPKGA
                     KGLMQLMPDTARRYGVDNPLDPKQNIQGGSAYLRYLLTLFGDDLQLALAAYNAGEGAV
                     MEYGHRIPPYRETTQYVPKVLSYYKRYKTMM"
     gene            complement(2716..3606)
                     /locus_tag="VD791_01125"
     CDS             complement(2716..3606)
                     /locus_tag="VD791_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXV06605.1"
                     /translation="MPRDHHTLPRSTWVLLAALTLGWGCNWPMMKLALAEVPVWTLRA
                     VSLAAGAAGMFLIAAAGGLRLLPPRDQWPGLALSAAFNITLWNLLVAYGLTYLPAGRA
                     VILAYTMPLWAVLLARVVLREPLSARRALGLSLGMLGMMLLIGDELTAVRAAPVGALL
                     VVGAALAWAIGTVLMKRFPTRLPITAFTGWQLAIGGAPIALGALALDWGDWRPLSWQA
                     GAAVAYNVLVAFVLCHWMWFKIVSRASAGVSALGTMTIPVAGVASSMLVLGERPGWQE
                     YAALLLVIAALATVLVPVKR"
     gene            3664..4023
                     /locus_tag="VD791_01130"
     CDS             3664..4023
                     /locus_tag="VD791_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HXV06606.1"
                     /translation="MQHPVVRFLLFWALNTLVLWVAAELFAGVRFDGWSALAISGLLF
                     GIAHAVLKPLLVILTLPITVVTLGLFLLVINALLLLLVAWLVPGFHLTGFWQAVLVGL
                     FISVFSFILNLLIRAEK"
     gene            4031..5293
                     /locus_tag="VD791_01135"
     CDS             4031..5293
                     /locus_tag="VD791_01135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV06607.1"
                     /translation="MSAERSGPSTAPGARTAAAVLLPFAAAYALSFLFRSVNAVIAPD
                     LVRRFALTPSQLGLLTSAYFLSFAAFQLPLGLLLDRYGPRRTDATLVLIAALGALVFA
                     VAPNAAVLIGGRALIGLGVSGCLMSGIKANVLWFPLARLPVMNGWMFFAGGLGLVLAT
                     LPVELLLQAIDWRRLFFALALLTMLSGLTVLRVVPERTEHTPRETFSAQLAGLRSVYA
                     ARRFWQLGLAALTSQATSMAVQGLWSGPWLRDVAGLGRQGVAAHLLLMALATMAGFLL
                     WGNVASWVARRGVPGARVFALGMWMFLVVQALLVFGAPVAPALIWIGFGVFGTAGSLS
                     YAVLSWEFPRRLAGRVTTALNALVFGWAFVVQWTIGGIIELWPVVDGRYHADGYRAAF
                     GVFLAVQALAFGWMLLDGWRLRRAHATR"
     gene            5329..6279
                     /locus_tag="VD791_01140"
     CDS             5329..6279
                     /locus_tag="VD791_01140"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXV06608.1"
                     /translation="MKIATAVAALALAVAAAPASAQSWQPPAEAQRCPSKWGAGDERG
                     AANHMKPTTVMRAAQLIKTGQVIELGRVLESNMPLFGTRRFEIHTKRTGPVLGGNKRR
                     SNEELVITEIGQVGTQFDMFSHQTIGNQFYNCFDNDEIATRNGFTKLGIDKIGTLFTR
                     GVLIDVAALKGVPTLPAGYEITTADLQGALQRQGVSLQAGDAVLIHSGYGKLWNVDNV
                     TYNKDTPGIGVPAAEWLAKQDVMLIGADTFAVEAAPNPDKNVSLPVHQIALVVNGIFL
                     LENLKLDELAAARAYEFAFIVQPLKVKGGTGSTVAPVAVR"
     gene            complement(6281..>6464)
                     /locus_tag="VD791_01145"
     CDS             complement(6281..>6464)
                     /locus_tag="VD791_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06609.1"
                     /translation="DARCFAQASRRAAIDHIVVSRSLAARLVPGSMRRVTYATADVRR
                     FRLSDHCPVAVTLELP"
BASE COUNT          908 a         2200 c         2305 g         1051 t
ORIGIN      
        1 cggccgcaac agggcggtgg ccgcgcgccc gacgatcttg gccttgaaca ccggcttcat
       61 gtcgtggtac atgaagccgc gtgccccggt ggcttcctcc acggcatcgg cgatgttgcc
      121 ggtgctggag ctgggcttgc gcagggcggc gatgatctgc tcgggggaca gcgccggctc
      181 ggcgcgcacc ggcaggctgg gcgcggcagc gccggcgagc gcgcacagca gcccgaacga
      241 ggcggcgcgt tgcatcggga cggtcctcca gggatgggtg cgacgatgtt accgcaagtc
      301 ccgccggcgg ggctcgcgcg gccggctttc acgccgccgg ctttcacgtc aagggggcgg
      361 caggcgaggt gaggccatga gcagcgcgga gttttccggc atctacccga tgcagtacgc
      421 cttcttcgat cgcggcggcg gcctcgaccg cgcggcgatg cgccggcagg tcgaggcggc
      481 gctggccggg gcgcgctgcg cgtgttcaac gggcgcggcg ggaacgagct gccggacaac
      541 ctgcgcgccg ggtgcgccgg cctgatcccg gccaccgaca ccttcgactt ccaggtgcgc
      601 gtcttcgagc tgatgcgaag cgggcgcgcg caggacgaag cgcgggccga agccgtctac
      661 cgcgaggtgc tgccggcgat cgtgttcgcg atgcagtcgc tcgatacctt gatctgttac
      721 ggcaagcgtc tcgcggcgct gcgcctgggc ctgcccgagg tgcacgaccg cgccccggcg
      781 ctcgcgctca ccgcatccgg cctggcgtgc gtgcgccgct atgccgccgc cctggggttt
      841 cttgtctaat aaagagcgtc cgctcgtctg aacgagaccc gctcgtccga accctggtga
      901 aacggcgtca actcggcggc gcgtggcgtt gctcttgctt ttcaggcgcg cacacactgc
      961 gccgcggcgg ccgctccgcg gaaacctccg cggcgcacgg atgctttcac aatgggtgat
     1021 gaattccttc cgagcgaagc ggaagccaag gtcgtcaacg gctggatcgc gctgctgatc
     1081 gtgctggcgc tgctcgcctt cggcgtgtac tttctcgccg ccggcctgcc gcgcagcgag
     1141 ggcggaccgc tcggtaccgg gctggcgctg gtcacggtcg gcctgatctg cctgcacggc
     1201 atgttcacgc tgcagcccaa cgaggccgcg gtgatgactt tcttcggcag ctacgccggc
     1261 accgagcgcc acgccggctt ccactgggcc aatccgttct gccagcggcg ccgcatgtcg
     1321 ctgcgcgcgc acaatctgac cacgccgatc ctcaaggtga acgacgggcg cggcagcccg
     1381 atcgagatcg gcgcggtggt ggtgtggcgc gtgcaggaca ccgcgcgcgc ctgcttcgag
     1441 gtggaggact acaccgagta cgtgcgcctg cagtgcgagt cgggtctgcg cgaggtggcc
     1501 agccgccacg cctacgacaa cgccgacgag accctgctcg gcgggcgcgt gcgcacgctg
     1561 cgcggcgatg ccgagcgcat ctccgagacg ctgcgtgcga cgatccaggc ccgcgccggc
     1621 ctcgccggcg tcgcggtgga ggaagcgcgc atcgcccaca tcgcctacgc gcccgagatc
     1681 gcgcccgcca tgctgcgccg ccagcaggcc gaggcggtga tcgccgcccg ccgcaaactg
     1741 gtggagggcg ccgtcggcat ggtcgagatg gcgctgatcg acatcaacga gcggtgcctc
     1801 gccagcttca cgccggcgca gaaggtggcg ctggtcacca acctgatgac cgtgctggtg
     1861 ggcgaagggc agccgcagcc ggtggtgccg atgaagcagg agagctgagg cgcagaccgc
     1921 cccggctctt ctctacatca tcgtcttgta gcgcttgtag tacgacagga ctttcggcac
     1981 gtactgcgtg gtttcgcggt acggcgggat gcggtgaccg tactccatca ccgcgccttc
     2041 gcccgcgttg taggcggcga gcgccagttg caagtcgtcg ccgaacaggg tgagcaggta
     2101 gcgcaggtag gccgagccgc cctggatgtt ctgcttcggg tccagggggt tgtccacgcc
     2161 gtagcggcgc gcggtgtcgg gcatcagctg catcaggcct ttggcgccct tgggcgaacg
     2221 cgccagcggg ttgtagccgg attcggccga gatcaccgcg tgaatcagcg cgggctcgag
     2281 cttgaaggcg cgcgccgcgg ccaccacgtg cgcgtgatac ttcttgcggt cggcgggcga
     2341 gtacgggcgg tagcggtgcg tgatggtgtt ggccaccgcg ctcggttcct tctcggtggc
     2401 gaggtagagc ttgaagcggc tgtccggggg cacgttggtg aagtgcacct tgccgtcctg
     2461 gtccacgtag cggtagatgt cggcatgaac cggcaacgca tatgccatca gcattgcgcc
     2521 tgcgatccag ccgatccttc gcctgtgcgg aatcatccct gtaccactct gtttttacgg
     2581 ggcttttctg ccctcgggcc ggggccggag ggtctgccgg tgagaaaagc aaagacggtg
     2641 ccacgaagag aatcggggat cgggaagcag accggcggtc gatcgcgccc tgctggacgg
     2701 tcgctaggcc gcgggctagc gcttgacggg gaccagaacg gtggcgagcg cggcgatgac
     2761 caggagcagg gcggcgtact cctgccagcc gggtctctcg ccgagcacca gcatgctgct
     2821 cgccacgccg gccacgggaa tcgtcatggt gccgagcgcg gatacgccgg ccgaggcgcg
     2881 gctgacgatc ttgaaccaca tccagtggca caggacgaag gccaccagca cgttgtacgc
     2941 gaccgcggcg ccggcctgcc agctcagcgg gcgccagtcc ccccagtcga gggcgagcgc
     3001 gccgagcgcg atgggtgcgc ctccgatcgc cagctgccag ccggtgaacg cggtgatggg
     3061 caggcgcgtg ggaaagcgtt tcatcagcac cgtgccgatc gcccaggcga gcgcggcgcc
     3121 cacgacgagc agcgcgccca ccggcgccgc ccgcaccgcg gtgagctcgt cgccgatcaa
     3181 cagcatcatg cccagcatgc cgagcgacag ccccagcgcg cggcgcgcgc tgagcggctc
     3241 ccgcagcacc acgcgcgcga gcagcaccgc ccacagcggc atggtgtacg ccaggatcac
     3301 ggcgcgcccg gcgggcagat aggtcaaccc gtaggcgacc agcaggttcc acagcgtgat
     3361 gttgaacgcg gcgctgagcg caaggcccgg ccattgatcg cgcggcggga gcagacgcag
     3421 gccgccggcg gccgcgatca ggaacattcc cgccgcgccc gcggcgaggg acacggcgcg
     3481 cagcgtccac accggcacct cggccagcgc cagcttcatc atcggccagt tgcagcccca
     3541 gccgagcgtg agcgccgcga gcagcaccca ggtggagcgg ggaagcgtgt ggtgatcgcg
     3601 gggcatcggt gcggagtcgg gctgccggat gatagccgag cgggatgcta cactgcgcgc
     3661 ctcatgcagc atcctgtcgt ccgcttcctg ctgttctggg cgctcaacac gctggtgttg
     3721 tgggtggccg cggagctgtt cgccggcgtg cgtttcgacg gctggagcgc gctcgcgatc
     3781 tcgggactgc tgttcggcat cgcgcacgcg gtgctcaagc cgctgctggt gatcctcact
     3841 ctaccgatca ccgtcgtcac gctgggcctg ttcctgctcg tcatcaatgc gctgctgctg
     3901 ttgctggtcg cctggctggt cccgggcttc cacctgaccg gtttctggca ggcggtgctg
     3961 gtcgggctgt tcatctccgt tttcagcttc atcctgaacc tgctgatccg cgccgagaag
     4021 tgaggcgcac atgagcgcgg agcgctccgg tccgtccacc gcgcccggcg cacgcaccgc
     4081 ggccgcggtg ctcctgccgt tcgccgcggc ctatgcattg tcgttcctgt tccgcagcgt
     4141 caacgccgtg atcgcacccg acctggtgcg ccggttcgcg ctcacgccca gccagctcgg
     4201 gttgctgacc agcgcctact tcctgtcctt cgcggctttt cagctgcccc ttggcctgct
     4261 gctcgaccgc tacgggccgc gccgcaccga cgccacgctg gtgctgatcg ccgcgctcgg
     4321 ggcgctggtg ttcgccgtcg cgcccaacgc cgctgtgctg atcggggggc gggcgctgat
     4381 cgggctgggc gtgtcgggct gcctgatgtc gggcatcaag gcgaacgtgc tgtggtttcc
     4441 gctggcgcgc ctgccggtga tgaacggctg gatgttcttt gccgggggct tggggctggt
     4501 gctcgcgact ctgccggtgg agctgctgct ccaggccatc gactggcgcc gcttgttctt
     4561 cgcgctggcg ctgctcacga tgctgtccgg cctgaccgtg ctgcgcgtgg tgcccgagcg
     4621 caccgagcac acgccgcgcg aaacgttttc cgcgcagctc gccggactgc gctcggttta
     4681 cgccgcccgg cgcttctggc agttggggct cgctgcgctc acttcgcagg cgaccagcat
     4741 ggcggtccaa gggctctggt ccggcccctg gctgcgcgac gtggccgggc tcggccgcca
     4801 gggtgtggcc gcgcatctgc tgctcatggc gctcgccacc atggccggct tcctgctctg
     4861 gggcaacgtc gcatcctggg tggcgcgccg cggtgtgccg ggcgcgcgcg tgttcgcgct
     4921 gggcatgtgg atgttcctgg tggtgcaggc tctgctggtg ttcggggcgc cggtcgcgcc
     4981 ggcgctcatc tggatcgggt tcggcgtgtt cggcaccgcc ggcagcctgt cctacgcggt
     5041 gctgtcctgg gagttcccgc gccggcttgc agggcgcgtg accaccgcgc tcaacgctct
     5101 ggtgttcggc tgggccttcg tggtgcaatg gacgatcggc ggcatcatcg agctttggcc
     5161 ggtggtcgac gggcgctatc acgccgacgg ctatcgcgcc gcgttcggcg tgttcctcgc
     5221 cgtgcaggcg ctcgccttcg gctggatgct gctcgacggc tggcgtctgc gtcgcgcgca
     5281 cgccacgcgc taaactagcg gccgcgaacc aacacgggag gagacatcat gaagatcgca
     5341 actgccgtcg ccgcactcgc gcttgccgtg gccgcggccc cggcctccgc gcagtcctgg
     5401 cagccgcccg cggaggcaca gcgttgtccg tccaagtggg gcgcgggcga cgagcgcggc
     5461 gccgccaatc acatgaagcc gacgacggtg atgcgcgccg cgcagctgat caagaccggc
     5521 caggtgatcg agctggggcg cgtgctggag tccaacatgc cgctgttcgg cacgcgccgc
     5581 ttcgagatcc acaccaagcg caccggtccg gtgctgggcg gcaacaagcg ccgcagcaac
     5641 gaggagctgg tgatcaccga gatcggccag gtgggcacgc agttcgacat gttctcccac
     5701 cagaccatcg gcaaccagtt ctacaactgc ttcgacaacg acgagatcgc cacgcgcaac
     5761 ggcttcacca agctcggcat cgacaagatc ggcacgctgt tcacccgcgg cgtgctgatc
     5821 gacgtggccg cgttgaaggg cgtgccgacg ctgccggccg gctacgagat cacgaccgcc
     5881 gatctgcagg gcgcgctgca gcgccagggc gtctcgctgc aggccggcga cgcggtgctg
     5941 atccactctg gctacggcaa gctgtggaac gtggacaacg tcacctacaa caaggacacg
     6001 ccgggcatcg gcgtgccggc cgccgagtgg ctggcgaagc aggacgtgat gctgatcggc
     6061 gccgacactt tcgccgtgga ggccgcgccc aaccccgaca agaacgtcag cttgccggtg
     6121 catcagatcg cgctggtggt gaacggcatc tttctgctcg agaacctgaa gctggacgag
     6181 ctggccgcgg cccgggccta cgagttcgcc ttcatcgtgc agccgctcaa ggtcaagggc
     6241 ggaaccggct cgacggtggc gccggtggcg gtacgatgac ctagggcagc tccagcgtca
     6301 ccgccaccgg gcagtgatcc gacagccgga accttctcac gtcggccgtc gcgtaggtca
     6361 cgcggcgcat cgatcccggc accagccggg ccgccagcga acggctgacc acgatgtggt
     6421 cgatcgccgc gcggcgcgag gcctgcgcga agcagcgcgc gtcg
//
LOCUS       DASPGE010000035         5890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1181194, whole genome shotgun sequence.
ACCESSION   DASPGE010000035 DASPGE010000000
VERSION     DASPGE010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181194"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VD791_01150"
     CDS             <1..412
                     /locus_tag="VD791_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06610.1"
                     /translation="MPEKRHTAYRHQQDSRRRAYLASRSLREELPRVERLVLQMTFVD
                     PRGAAQHSPQMHTFSRGAKAFFAIPCPCSLCLDGGFDLGPAVKRMLAEGSEESTGRLL
                     CPGWQNPERTEKDRCRIEMRYRLSASYRPPGGVD"
     gene            complement(485..2131)
                     /locus_tag="VD791_01155"
     CDS             complement(485..2131)
                     /locus_tag="VD791_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06611.1"
                     /translation="MQNRLSHWLAAMVLGGGAMLAHGHEGDDVPAGKPGEQLGKVVFR
                     VDCSPAAQREFNRAVALYHSFWFDPATASFRKVLEFDPGCGMGWWGQALSALANPFGW
                     PAPSKAMRAGAAAIEQGLKTGARTQRERDYIAALAKLFQDWDKTDHRPRALAWEAAME
                     QVAQRYPDDDEAQIFYALSLVANALASDKTFSRQLKAGEILEPYFQSHPDHPGAAHYL
                     IHAYDYTELVQRGLPAARRYASIAPSAPHALHMPAHIFTRLGLWEDSIATNSASARAA
                     KAELESASHTLGSYNAVHAMDYMVYAHLQLGQDAAARRLIEEIAAIERVDATNFAAAY
                     GFAAMPARYALERRRWDEAARLELRPQSLPWNQFPHAEAIVVYARALAKARLGEAPGA
                     RSDIARLQQLRDALATMKLGYWAQQVEIQITAASAWLALAEGRQGDALGLMRQATEME
                     TATDKHPVTPGPLVPTRELLGEMLLEQGQPAQALAEFERLERAEPNRLLPLYYAARAA
                     EAVGDTAKAQSYYRKLLELAAKRDTERPEIARARAHVALN"
     gene            complement(2166..2537)
                     /locus_tag="VD791_01160"
     CDS             complement(2166..2537)
                     /locus_tag="VD791_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HXV06612.1"
                     /translation="MSTGDPLALPGIPRDDAGPVFRAPWEAQAFGMTLALFERGCFTW
                     REWTQRLAAQIAAAQIAATGARGEHDDGSRYYEHWLAALEQLAADKGLASLREMAERK
                     MAWDAAARATPHGRPIVLPGR"
     gene            complement(2576..3247)
                     /gene="nthB"
                     /locus_tag="VD791_01165"
     CDS             complement(2576..3247)
                     /gene="nthB"
                     /locus_tag="VD791_01165"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HXV06613.1"
                     /translation="MNGVHDMGGMHGFGPVEPERDEPVFHHDWEKKVFALRFASGAMG
                     KWNIDMARHANERMPPAEYLAATYYERWLNGLERLLLERGVVTRRELETGRSDAPLPA
                     EDRARVLRAGAVDGVLAAGRNYRVDEDVAPRFAAGAAVMTRNIHPAGATRLPRYARGR
                     RGVVERDHGVFIFPDASAAGGRKPQHLYSVRFRTRELWGEAASPRDAVYLDLWDDYLD
                     PAQPA"
     gene            complement(3244..3882)
                     /gene="nthA"
                     /locus_tag="VD791_01170"
     CDS             complement(3244..3882)
                     /gene="nthA"
                     /locus_tag="VD791_01170"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HXV06614.1"
                     /translation="MSHSDPEHDHEHSGGSRFSEVELRVKALESLLVDKGVVDPAALD
                     RLIDSYEHRIGPRNGARVVARAWTDPAYRQRLQHDATAAIAELGYSGAQGEHMVVVEN
                     TARVHNLVVCTLCSCYPWPVLGLPPVWYKSNAYRARVVVDPRGVLRDFGLELADDVEV
                     RVWDSTAEIRYLVLPERPAGTEGLSEAQLAELVTRDSMVGVAKASAAPGGKL"
     gene            complement(3902..4534)
                     /locus_tag="VD791_01175"
     CDS             complement(3902..4534)
                     /locus_tag="VD791_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HXV06615.1"
                     /translation="MTAGRHLFRSLLLAFATYCAAPVLAAQDQAVSANPTLSVIGRAP
                     DFALKDTGGETVRLAGYRGRVVLLAFVFTTCPGVCPVISQQMAGLQARLKQERLFGSK
                     AAMLSVTVDPGTDTAEVLARYARTFGADPAGWRFLREDAPKLDPVLKAYDEWTKLLPP
                     GRGAQAAQLDHPARVYLIDPAGNIREIYSLAFFNDKQALLDMKKLLSELR"
     gene            complement(4543..5778)
                     /locus_tag="VD791_01180"
     CDS             complement(4543..5778)
                     /locus_tag="VD791_01180"
                     /inference="COORDINATES: protein motif:HMM:NF017505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HXV06616.1"
                     /translation="MRFHPTKLAVAVAVAFASAVAIPLDESAAETCISPYIKALNQPE
                     KVMYLWALPATPGGGEDFLAVIDVNLASATYGKVLKKVYVGSKGNEAHHVGYTDDRTK
                     LWAASLNSDRFFVFDVASDPMNPKLLKVIDNVAQLTGLTGPHTPYAIPGRMLVSMASG
                     PNGKGPGGLAEFTNDGKFIASHKATNHPYETVVKPEFNRMITSAWFPQETWMKPLDKW
                     DPSTWSNPNTMLVWDLKERKIIQTLTAPDAVMLAARWMLKPGARYGYNISNAGNSIWM
                     FKMRDDGTFDYRKAADVGAGCGPADLRQSPDDKYLYLTCFVSSEIQAWDISDPENIKL
                     HDTIQGVVQPNMMHVTYDGRRLYFTNSAISSIDYSPRYSMQLVQIGRDGRMKLDPNFR
                     IDFSKAPDGPARPHDMLLN"
BASE COUNT          842 a         2087 c         2016 g          945 t
ORIGIN      
        1 catgcccgag aagcggcaca ccgcctaccg gcaccagcag gactcccggc gtcgcgccta
       61 cctggcctcg cgcagcttgc gcgaggagct gccgcgggtc gagcggctgg tgctgcagat
      121 gaccttcgtc gatccgcgcg gcgctgcgca gcactcgccg cagatgcaca ccttctctcg
      181 cggcgccaag gctttcttcg ccatcccctg cccttgctcg ctgtgtctcg acggcggctt
      241 cgacctggga ccggcggtca agcgcatgct cgccgagggc tcggaagaga gcaccgggcg
      301 gctgctgtgt cccgggtggc agaatccgga gcgcaccgag aaggaccgct gccgcatcga
      361 gatgcgctat cgcctgtcgg ccagctaccg gccccccggc ggcgtggact gagcggcgcg
      421 agcaggagat cccggccgtc ggtgtctgca gcgccgcgcg tgcggcgcga ggccgtgcct
      481 gccttcagtt cagcgccacg tgcgcgcgcg ctcgcgcgat ctccggccgc tcggtgtcgc
      541 gcttggcggc gagctcgagc agcttgcggt agtagctctg cgccttggcg gtgtcgccga
      601 ccgcttcggc ggcgcgcgcg gcgtagtaca gcggcagcag ccggttcggc tcggcccgct
      661 ccaggcgctc gaattcggcc agcgcttggg ccggctgccc ctgctccagc agcatctccc
      721 ccagcagctc gcgcgtgggc accagcgggc ccggcgtgac cggatgcttg tcggtggcgg
      781 tctccatctc ggtggcctgg cgcatcaggc cgagcgcgtc gccctggcgg ccctcggcga
      841 gcgccagcca ggccgaagcg gcagtgatct ggatctcgac ctgctgcgcc cagtagccga
      901 gcttcatcgt cgcgagcgcg tcgcgcagct gctgcaggcg cgcgatgtcg ctgcgcgcgc
      961 cgggcgcctc gcccaggcgc gccttggcca acgcgcgcgc atagacgacg atggcttccg
     1021 catgcgggaa ctggttccac ggcaggctct gcggccgcag ctcgagccgc gccgcctcgt
     1081 cccagcgccg ccgctccagc gcatagcgag ccggcatggc ggcgaagccg tacgcggccg
     1141 cgaaattggt cgcgtccacg cgctcgatcg ccgcgatctc ttcgatcagg cggcgcgcgg
     1201 ccgcgtcctg cccgagctgc agatgcgcgt acaccatgta gtccatggcg tgcaccgcgt
     1261 tgtaggagcc cagcgtgtgg ctggcggact cgagttccgc cttcgccgcc cgggccgacg
     1321 cgctgttggt cgcgatcgaa tcctcccaca ggcccagccg ggtgaagatg tgggcgggca
     1381 tgtgcagcgc gtgcggcgcc gaaggcgcga tcgacgcgta gcggcgcgcc gccggcagcc
     1441 cgcgctgcac cagctcggtg tagtcgtagg cgtggatcag gtagtgcgcg gccccggggt
     1501 gatccgggtg cgactggaag tacggctcca ggatctcgcc ggccttgagc tggcgcgaga
     1561 aggtcttgtc ggaagcgagc gcgttggcca ccagcgacaa cgcgtagaag atctgcgcct
     1621 cgtcgtcatc cgggtagcgc tgcgcgacct gctccatcgc cgcctcccag gccagcgcgc
     1681 gcggccggtg gtcggtcttg tcccagtcct gaaacagctt ggcgagcgcc gcgatatagt
     1741 cgcgctcgcg ctgcgtgcgc gcaccggtct tgagcccctg ctcgatcgcc gcggcgccgg
     1801 cccgcatcgc cttggaaggc gccggccagc cgaacggatt ggcgagcgcg ctgagcgcct
     1861 gcccccacca ccccatgccg cagccggggt cgaactcgag caccttgcgg aacgacgcgg
     1921 tggcgggatc gaaccagaag gagtgataca aggcgacggc gcggttgaac tcgcgctgcg
     1981 cggccgggct gcagtcgacg cgaaaaacga ccttgcccag ctgctcgccg ggcttgccgg
     2041 cgggaacgtc gtcgccctcg tggccatgcg cgagcatggc gccgccgccg aggaccattg
     2101 ctgcgagcca atgcgagaga cggttttgca tcgttgccct cctggttgcg tgacccttcg
     2161 attcctcacc tgccgggcag gacgatcggt cggccgtgcg gggtggcgcg cgccgcggcg
     2221 tcccaggcca tcttgcgctc ggccatctcg cgcaggctcg ccaggccctt gtcggcggcg
     2281 agctgctcga gcgcggccag ccagtgctcg tagtagcggc tgccgtcgtc gtgctcgccg
     2341 cgcgcccccg tcgccgcgat ctgggccgcc gcgatctgcg ccgcgagccg ctgcgtccac
     2401 tcgcgccagg tgaagcagcc gcgctcgaac agcgccagcg tcatgccgaa cgcctgcgcc
     2461 tcccacggcg cgcgaaacac cggccccgcg tcgtcgcgtg gaataccggg cagtgcaagc
     2521 ggatcgccgg tgctcatgtc ccagtgctca tatcccggtg ctcatctcga tacgctcaag
     2581 cgggctgcgc cggatccagg taatcgtccc acaggtccag gtacacggcg tcgcgcggcg
     2641 aggcggcttc cccccacagt tcgcgcgtgc ggaaccgcac gctgtacaaa tgctgcggct
     2701 tgcgcccccc ggccgcgctc gcgtccggga agatgaacac gccgtggtcg cgctcgacca
     2761 cgccgcgccg gccgcgcgca taacgcggca gccgcgtggc gccggcggga tggatgttgc
     2821 gcgtcatgac ggcagcgccc gccgcgaagc gcggcgcgac gtcctcgtcc acgcggtagt
     2881 tcctgccggc ggccagcacg ccgtccaccg cgccggcacg caggacgcgg gcgcggtcct
     2941 cggccggcaa cggcgcgtcg ctgcgccccg tttccagctc gcggcgcgtc accacgccgc
     3001 gctccagcag caagcgctcc agcccgttca gccagcgctc gtaatacgtc gcggcgaggt
     3061 actcggccgg cggcatgcgc tcgttggcgt gccgcgccat gtcgatgttc cacttgccca
     3121 tcgccccgga cgcgaagcgc agcgcgaaca ccttcttctc ccagtcgtgg tggaacaccg
     3181 gttcgtcgcg ttccggctcc accgggccga acccgtgcat cccgcccatg tcgtgcacgc
     3241 cgttcacagc ttgccgcccg gcgccgcgct cgccttcgcc acgccgacca tcgaatcgcg
     3301 cgtcaccagc tcggcgagct gcgcttcgct cagcccctcg gtgccggccg gccgctcggg
     3361 cagcaccagg tagcggatct ccgcggtgct gtcccagacg cgcacctcca cgtcgtcggc
     3421 aagctccagg ccgaagtcgc gcagcacgcc gcgcggatcg acgaccacgc gcgcgcggta
     3481 ggcgttggat ttgtaccaga cgggcggcag tcccagcacc ggccagggat agcaggagca
     3541 cagcgtgcac accaccagat tgtgcacgcg cgcggtgttc tccaccacca ccatgtgctc
     3601 gccctgcgcg ccgctgtagc ccagctcggc gatggcggcc gtggcgtcgt gctgcagccg
     3661 ctgccggtag gcgggatcgg tccaggcgcg cgccaccacc cgcgcgccgt tgcgcggtcc
     3721 gatgcgatgc tcgtagctgt cgatcagccg gtcgagcgcc gccggatcga ccacgccctt
     3781 gtccaccagc agggactcca gcgccttgac gcgcagctcc acctcgctga agcgcgagcc
     3841 gccggaatgt tcatggtcat gctcgggatc ggaatggctc atcgcggtgc aacgccggtc
     3901 gctagcgcag ttcggacagc agcttcttca tgtccagcag cgcctgcttg tcgttgaaga
     3961 aagccaggct gtagatctcg cggatgttgc ccgcgggatc gatcaggtac acgcgcgccg
     4021 gatgatccag ctgcgccgct tgcgccccgc gtcccggcgg gagcagcttg gtccactcgt
     4081 cgtaggcctt gagcaccgga tcgagcttgg gcgcatcttc gcgcagaaag cgccagccgg
     4141 ccggatcggc gccgaaggtc cgggcgtacc tggccagcac ctccgcggtg tcggtgccgg
     4201 gatccacggt caccgacagc atcgcggcct tgctgccgaa cagccgctcc tgcttcagcc
     4261 gcgcctgcaa ccccgccatc tgctgcgaga tcaccgggca cacgccgggg caggtggtga
     4321 agacgaacgc gagcagcacc acccgtcccc ggtacccggc gagccgcacc gtctcgccgc
     4381 cggtgtcctt cagcgcaaag tcgggcgcgc ggccgatgac cgacagcgtc gggttggcgg
     4441 agacggcctg gtcttgcgcc gcgagcaccg gcgccgcgca gtacgtggcg aacgcgagca
     4501 acaggctgcg aaacagatgc cggccggccg tcatgcccgc actcagttga gcagcatgtc
     4561 gtgcggccgc gccggcccgt cgggcgcctt gctgaaatcg atcctgaagt tcggatcgag
     4621 cttcatgcgc ccgtcgcgcc cgatctggac cagctgcatc gagtagcgcg gcgaataatc
     4681 gatgctgctg atcgcagagt tggtgaagta caggcgccgc ccgtcgtagg tcacgtgcat
     4741 catgttgggc tgcaccacgc cctggatggt gtcgtgcagc ttgatgttct ccggatcgga
     4801 gatgtcccag gcctggatct cgctgctcac gaagcaggtc aggtacaggt acttgtcgtc
     4861 gggcgactgg cgcaggtcgg caggcccgca gcccgcgccc acgtcggccg ccttgcggta
     4921 atcgaaggtg ccgtcgtcgc gcatcttgaa catccagatc gagttgccgg cgttgctgat
     4981 gttgtagccg taacgcgccc cgggcttgag catccagcgc gcggccagca tcacggcatc
     5041 gggcgcggtg agcgtctgga tgatcttgcg ttccttcaag tcccacacca gcatggtgtt
     5101 ggggttggac caagtgctcg ggtcccactt gtcgagcggc ttcatccagg tttcctgcgg
     5161 gaaccaggcc gaggtgatca tgcggttgaa ctcgggcttc accaccgtct cgtacggatg
     5221 attggtcgcc ttgtggctgg cgatgaactt gccgtcgttg gtgaactccg ccaggccgcc
     5281 cgggcccttg ccattgggcc ccgaggccat gctgaccagc atgcggccgg ggatggcgta
     5341 gggcgtgtgc gggccggtca gcccggtgag ttgcgcgacg ttgtcgatga ctttgagcag
     5401 cttggggttc atcgggtcgc tggccacgtc gaacacgaag aagcggtcgg aattgagcga
     5461 cgccgcccag agcttggtgc ggtcgtcggt gtagccgacg tggtgcgcct cgttgccctt
     5521 cgagccgacg taaaccttct tcagcacctt gccgtaggtc gccgaggcga gattgacgtc
     5581 gatcaccgcg aggaaatcct cgccgccgcc gggcgtggcc ggcagcgccc acaggtacat
     5641 gaccttctcc ggctggttga gcgccttgat gtacggcgag atgcaggtct cggccgcgct
     5701 ttcgtccaag gggatggcca ccgcagaggc gaacgctaca gcgacggcta ccgccagctt
     5761 cgtcggatgg aatcgcatga aagaagtctc cttggtccgt tgatcgatga tccgcacaag
     5821 tcgacgcccg gaatcataaa ccgggcgcca caacacgcaa gtctgcatgc cggccacggg
     5881 ttggccatat
//
LOCUS       DASPGE010000036         4634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_830290, whole genome shotgun sequence.
ACCESSION   DASPGE010000036 DASPGE010000000
VERSION     DASPGE010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830290"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /gene="coaE"
                     /locus_tag="VD791_01185"
     CDS             complement(<1..505)
                     /gene="coaE"
                     /locus_tag="VD791_01185"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HXV06617.1"
                     /translation="MPFVVGLTGGIGSGKSAAADEFARLGAAVIDTDAIAHELTAAGG
                     AAIDEVRRVLGDAYIDDTGAMDRAKVRARVFSDAAARARLEAVLHPRIRAESERRIAA
                     ARAPYVVHVVPLLLESGDYRRRVARVLVVDCAPELQAARVRRRSGLAEDEIRRIIAAQ
                     MPRAARLA"
     gene            complement(505..1371)
                     /locus_tag="VD791_01190"
     CDS             complement(505..1371)
                     /locus_tag="VD791_01190"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HXV06618.1"
                     /translation="MAVASWLAQPAVLPWVALVLGVCVGSFLNVVIHRLPKMMEREWH
                     AQCAELAGAPAAPPAQGRYNLLVPGSACPACGRHIRAVENIPVLSWLLLRGKCAGCAA
                     RIPARYPVVEIAAGAIAAYAAWRFGLSLAAAGAMLFGWSMLALAAIDLDTQLLPDDIT
                     LPLLWAGLLFNLERVFVPLPTAVIGAVAGYLSLWLVYWAFKLATGKEGMGYGDFKLLA
                     AIGAWLGWQKLPLVILLSSVVGAAVGIALIVLARHGREKPIPFGPYLAAAGLIALFWG
                     EPITRRWLPILG"
     gene            complement(1372..2595)
                     /locus_tag="VD791_01195"
     CDS             complement(1372..2595)
                     /locus_tag="VD791_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXV06619.1"
                     /translation="MATAAAKAAGPKEFNYTWEGRDKGGKVIRGELRSVSEAAVNATL
                     RRQGIVVQKVRKQKIGRGGKVTEKDLALFTRQLATMMKAGVPLLQSFDIVSKGASNPA
                     VARLLVDIKTEVETGSALAAAFRKHPLYFDALYCNLVAAGEQAGILETLLERLAVYKE
                     KILAIKSKIKSALFYPIAIVVVAFVITAVIMIFVIPAFKEVFTNFGADLPAPTLMVMA
                     ISDWFVANWYIIFPVVIGSVIGFLEAWKRVLPVQIFMDRLMLKLPVFGDLVLKSTIAR
                     WTRTLATMFAAGVPLVEALDSVGGASGNHVYLTATKKIQQEVSTGTSLTMAMQNSGVF
                     PNMVLQMCSIGEETGALDSMLSKVADFYEAEVDDAVEALSSLMEPMIMVVLGTLIGGM
                     VIAMYLPIFKMGQAV"
     gene            complement(2734..4026)
                     /locus_tag="VD791_01200"
     CDS             complement(2734..4026)
                     /locus_tag="VD791_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HXV06620.1"
                     /translation="MGDLPLVWGFVALGVLLVLSGFFSIAETSMMALNRYRLRHLVAQ
                     NHGGARRAAALLKRTDTLLGAILIGNNLVNAAAATLAGLIAVELLGQDRFAYAVSTLS
                     ISFLIIVFAEITPKVIGATYPERIALPLAYVLGPLRQTLQPGVWFVNLFVRPLLLAAG
                     IRPGENTEVQKLTPEEIRTLVLESSSFMPKKHVSILLNLFDLGSITVQDIMLPRARIE
                     SVKLEDDMATIARQLGTSYHMRLPVFRDARGDVLGVLHLRKVLSPLLAGTLDKAQIEA
                     LLVPPYFVPASTPVFAQLQYFQENEERIALVVDEYGELMGLVTLEDIIEEIIGKFTTS
                     IPSAHALGWGEDGSASVDGATPVREVNRALGLELPTEGPKTMNGLILEHLRDIPEADV
                     SVKIGEVPMEIVHAQGRSVKTVRIFRPRGAVEEVTERA"
     gene            complement(4028..>4634)
                     /gene="ccsA"
                     /locus_tag="VD791_01205"
     CDS             complement(4028..>4634)
                     /gene="ccsA"
                     /locus_tag="VD791_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HXV06621.1"
                     /translation="IYWVESFFYDLDGILPPALLLAALASPLPALFPGSASAALAASL
                     EFRLHVVLAMLAYSLFTIAILHALLMALLERRLHGSAALRGAEGAAMLAGPLGALPPL
                     LTLERLLFRIIAAAFVLLTCTLATGIAFSESLFGRPLQFDHKTLFALISWATFALLLA
                     GRHLYGWRGRVALRWTLTGFVLLLLAYVGSRFVLEVLLGRG"
BASE COUNT          794 a         1583 c         1593 g          664 t
ORIGIN      
        1 ccgcgagtcg cgcggcccgc ggcatctgtg ccgcgatgat gcgccggatt tcatcctccg
       61 cgaggccgct gcggcgccgg acgcgcgccg cctgcagctc gggtgcgcaa tccacgacca
      121 gcacacgggc gacgcggcgc cggtaatcgc cggactcgag cagcagcggc accacgtgca
      181 cgacgtacgg cgcgcgcgcg gccgcgatgc gacgctcgga ctcggcgcgg atgcgcggat
      241 gcagcaccgc ttccagtcgc gcccgggccg ccgcgtcgga aaacacccgg gcgcgcacct
      301 tggcgcggtc catggcgccg gtgtcgtcga tataggcgtc gccgagcacg cggcgtactt
      361 cgtcgatggc ggcgccgccc gccgcggtca gttcgtgcgc gatggcgtcg gtgtcgatca
      421 cggcggcgcc gagccgcgca aactcgtccg ccgcggcgct cttgccgctg ccgatgccgc
      481 cggtcaggcc gaccacgaag ggcatcaacc gaggatcggc agccagcggc gcgtgatggg
      541 ctcgccccag aacagcgcga tgagtccggc ggccgcgaga tacgggccga agggaatggg
      601 cttctcccgg ccatggcgcg cgagcacgat cagcgcgatg ccgacggcgg cgccgaccac
      661 ggacgacagc aggatcacca gcggcagctt ctgccagccg agccaggcgc cgatcgcggc
      721 gagcagcttg aagtcgccat agcccatgcc ctccttgccg gtggcgagct tgaatgccca
      781 gtacacgagc cacagggaca ggtagccggc gacggcgccg atcaccgcgg tcggcagcgg
      841 cacgaagacg cgctccaggt tgaagagcag cccggcccac agcagcggca gcgtgatgtc
      901 gtccggcagg agctgggtgt ccaggtcgat cgccgccagc gcgagcatcg accagccgaa
      961 cagcatggcg cccgccgccg ccagcgacag gccgaagcgc caggcggcgt acgccgcgat
     1021 tgccccggcg gcgatctcga ccaccgggta gcgcgccggg atgcgtgcgg cgcagccggc
     1081 gcacttaccg cgcaggagaa gccagctgag caccgggata ttctccactg cgcggatgtg
     1141 ccggccgcag gccggacagg cggatccggg aacgagcagg ttgtagcggc cctgcgcggg
     1201 cggcgcggcc ggagcgcccg cgagctcggc gcactgcgca tgccactcgc gttccatcat
     1261 cttcggcaag cggtggatga cgacgttcag gaacgacccg acacacacgc cgagcaccag
     1321 cgccacccac ggcagaaccg ctggctgggc cagccagctc gcgaccgcca tctagacggc
     1381 ttggcccatc ttgaagatcg gcaggtacat ggcgatcacc atgccgccga tgagcgtgcc
     1441 gagcaccacc atgatcatcg gctccatcag cgaggagagc gcctcgacgg cgtcgtccac
     1501 ttctgcctcg tagaaatccg cgaccttgct gagcatgcta tcgagcgcgc cggtttcctc
     1561 gccgatcgag cacatctgca gcaccatgtt ggggaagacg cccgagttct gcatcgccat
     1621 ggtgagcgag gtgcccgtgg agacctcctg ctggatcttc ttggtggccg tgaggtagac
     1681 gtgattgccg gaggcgccgc ccaccgaatc aagcgcctcc acgagcggca cgccggccgc
     1741 gaacatggtg gcgagcgtgc gcgtccagcg cgcgatggtg gatttcagca ccaggtcgcc
     1801 gaacacgggc agcttgagca tcagccggtc catgaagatc tgcaccggca gcacgcgctt
     1861 ccaggcttcc aggaagccga tcacgctgcc gatcaccacg gggaagatga tgtaccagtt
     1921 ggccacgaac cagtccgaga tggccatcac catcagcgtc ggcgccggca ggtcggcgcc
     1981 gaagttggtg aacacctctt tgaacgccgg aatgacgaag atcatgatca cggcggtgat
     2041 gacgaaggcc acgacgacga tggcgatcgg gtagaacagc gccgacttga tcttcgactt
     2101 gatggcgagg atcttttcct tgtagaccgc cagccgctcg agcagcgtct ccaggatccc
     2161 cgcctgctcg cccgcggcca ccaggttgca gtacagcgcg tcgaagtaca gggggtgctt
     2221 gcggaaagcg gcggcaagcg ccgatcccgt ttccacctcg gtcttgatgt ccaccagcag
     2281 gcgcgccacg gccgggttgg aagcgccctt gctgacgatg tcgaaggact gcagcagcgg
     2341 cacgcccgcc ttcatcatgg tcgccagctg ccgcgtgaac agcgcaaggt ccttctcggt
     2401 gaccttgccg cccctgccga tcttctgctt cctgaccttc tggacgacga tgccctggcg
     2461 gcgcaatgtg gcgttcacgg ctgcctcgct gaccgagcgc agctcgccac ggatcacctt
     2521 gccgcccttg tcgcggcctt cccaggtgta gttgaattcc ttgggcccgg cggcctttgc
     2581 cgctgccgtt gccatagcgc gtaccccgat tccgttcccg tcgccgcagg ataggctgac
     2641 gatgatgccc tgcggcccgt gttttgtaaa gcctcaggtt tcgcgtctaa ccgactgacc
     2701 agaaaacatt ttgacctatc cggaaggcct ccctcaggcc cgttccgtga cctcctccac
     2761 agcgccccgc ggccggaaga tgcgcaccgt tttcacgctc cggccctggg cgtggacgat
     2821 ctccatcggc acctcgccga tcttcaccga gacgtccgcc tcggggatgt cgcgcaggtg
     2881 ctcgaggatg aggccgttca tggtcttcgg cccttccgtg ggcagctcca gcccgagcgc
     2941 gcggttcacc tcgcgcacgg gcgtggcgcc gtccaccgac gcgctgccgt cctcgcccca
     3001 gccgagcgcg tgcgcggacg ggatcgaggt cgtgaacttg ccgatgatct cctcgatgat
     3061 gtcctccagc gtcacgaggc ccatcagctc gccgtactcg tccaccacca gcgcgatgcg
     3121 ctcctcgttt tcctggaagt actgcagctg cgcgaacacc ggcgtgcttg ccggcacgaa
     3181 gtacggcggc acgagcagcg cctcgatctg cgccttgtcg agcgtgccgg cgagcagcgg
     3241 cgagagcacc ttgcgcagat gcagcacgcc cagcacgtcg ccgcgcgcgt cgcggaatac
     3301 cggcagacgc atgtggtagc tggtgccgag ctggcgcgcg atggtcgcca tgtcgtcctc
     3361 cagcttgacc gactcgatgc gcgcgcgcgg cagcatgatg tcctgcacgg tgatcgagcc
     3421 caggtcgaag agattcagca ggatggagac gtgcttcttc ggcatgaagc tcgaggactc
     3481 gagcaccagc gtgcggatct cctccggcgt gagcttctgc acctcggtgt tctcgccggg
     3541 ccggatgccc gcggcgagca gcagcggtcg cacgaacagg ttgacgaacc acacgcccgg
     3601 ctgcagcgtc tgacgcagcg gcccgagcac gtaggcgagc ggcagggcga tgcgctccgg
     3661 gtaagtggcg ccgatcacct tgggcgtgat ctcggcgaac acgatgatga gaaacgagat
     3721 gctcagggtg ctcacggcgt aggcgaacct gtcctggccg agcagctcga ccgcgatcag
     3781 gccggcgagc gtggccgccg ccgcgttcac caggttgttg ccgatgagga tggcgcccag
     3841 cagcgtgtcg gtacgcttga gcagcgcggc ggcgcgccgc gcgccgccgt ggttctgcgc
     3901 caccaggtgg cgcaggcggt agcggttgag cgccatcatg ctggtctcgg cgatcgagaa
     3961 aaagcccgag agcaccagca gcacgccgag cgcgacgaag ccccagacga gcggcaggtc
     4021 tcccatgtca gccccggccg aggagcactt ccagcacgaa gcggctgccg acgtaggcga
     4081 gcagcagcag cacgaacccg gtcagcgtcc agcgcagcgc cacgcggccg cgccagccgt
     4141 acaggtggcg cccggcaagc agcagggcga aggtcgccca ggaaatcagc gcgaacagcg
     4201 tcttgtggtc gaactgcagc ggccggccga acagcgactc ggaaaacgcg atgccggtgg
     4261 ccagcgtgca ggtcagcagg acgaaggcgg cggcgatgat gcggaacagc agccgctcga
     4321 gcgtgagcag cggcggcagc gcgccgaggg gcccggcgag catcgccgcg ccctcggcgc
     4381 cgcgcagggc agcgctgccg tgcagccggc gctcgagcag cgccatcagc agcgcgtgca
     4441 ggatggcgat ggtgaacagg ctgtaggcga gcatggcgag cacgacgtgc aggcggaact
     4501 ccagcgacgc ggcgagcgct gcgctggcgc ttccggggaa gagcgccggc aggggcgagg
     4561 ccagggcggc gagcagcaac gccgggggca ggatgccgtc caggtcgtag aagaagcttt
     4621 ccacccagta gatg
//
LOCUS       DASPGE010000037         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_362039, whole genome shotgun sequence.
ACCESSION   DASPGE010000037 DASPGE010000000
VERSION     DASPGE010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362039"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(353..2191)
                     /locus_tag="VD791_01210"
     CDS             complement(353..2191)
                     /locus_tag="VD791_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXV06622.1"
                     /translation="MTTHEVLPWQEQAEPEVGFAMDRFGNVSQMGAGWSMLTGGSPHD
                     VLSRPFADILHPADRPPVLEALQSLIRGEIYSCRMPARCLRRDGLHCRVEIYAHPTLD
                     ADGRIVGVRGNFTDITARRKSMRALRESEARFRAISDASPLGVYVTDAAGGCIFANAN
                     FEQISGLRADQVRASGHLSTLHPEDRTRVLLAREAATRSRTPYGVDCRYLHADATVAW
                     TRINGAPIRDGNNLLGFVHVVEDVSAQRCAGESLRRSQERLQLALEGSGDVLLDWDLR
                     SGEVYLSEQWGQLVGGPRGAAITTVRDLLELVHPREQPLVQQAVDETLSGERPFLRVQ
                     YRVRAQTGEWKWVETHARVMERGDDGTPLRVTGTSADITDRKNLEARQAEFMATVSHE
                     LRTPLASVLGALEILREDYLADLPEDACRFIEMALRNGNQLAGLIQSVLDLERIETGV
                     HGFDFAAVSVKELLGRALQVNEAFALKLAVRLEVEASDGTDCYVWTDMVRALQVLTNL
                     ISNAVKFSPAGQCVTLGSLCTADRVRLFVEDRGPGIPEDSRDCIFQRFGQASNQEHSR
                     LPGSGLGLSICKALVTRMGGDIGLHSQTGRGSVFWIELPKAKSPTA"
BASE COUNT          363 a          838 c          834 g          386 t
ORIGIN      
        1 acaccggcac gcggcgcgca gcctgcgcca tttcgccgac gaattctgcg ccgggcggct
       61 gatggccttc ggcggcggcg gttacgaccg gcgcaacctg gcgctggcgt ggtgcgaggt
      121 gctgcgggaa ttggtctgaa ggcgaggaaa ggcgtgaccg agaagcgtga gcgccgcccg
      181 tcatgtccgt agcccggatg cagcaaagcg gaatccgggg agcaagaaag cgctcttttc
      241 ccggattgcg ccgcccgttt tatcgcccag atgcttgggc ggctccatcc aggctacgct
      301 tccccatcac gcttctcggt cacgctcacg cttctcggtc acgcagtacc gatcacgcag
      361 tagggctttt cgccttcggc agctcgatcc agaacacgct gccgcgcccc gtttgcgagt
      421 gcaggccgat gtcgccgccc atgcgcgtga ccagcgcttt gcagatggac agccccagcc
      481 cgctgcccgg caggcgcgag tgctcctgat tggaggcctg gccgaagcgc tggaatatgc
      541 agtcgcgcga atcctcgggg ataccggggc cacggtcctc gacgaacaac cgcacgcggt
      601 cggccgtgca gaggctgccc agcgtgacgc actgtcccgc cggcgagaac ttcacggcgt
      661 tggagatgag attggtcagc acctgcagcg cgcgcaccat gtcggtccac acgtagcagt
      721 ccgtgccgtc cgacgcctcc acctccagcc gcacggcgag cttgagcgcg aaggcttcgt
      781 tgacctgcag cgcgcgcccg agcagctcct tcaccgacac ggcggcgaag tcgaagccgt
      841 gcacgccggt ctcgatgcgc tccaggtcga gcacgctctg gatcagcccg gcgagctggt
      901 tgccgttgcg cagcgccatc tcgatgaaac ggcaggcgtc ctcgggcagg tcggcgaggt
      961 agtcctcgcg caggatctcc agcgcgccca gcaccgaggc gagcggcgtg cgcaactcgt
     1021 ggctgacggt ggccatgaac tcggcctggc gcgcttccag gttcttgcgg tcggtgatgt
     1081 cggcgctggt cccggttact ctcaggggcg tgccgtcatc gccgcgctcc atgacgcgcg
     1141 cgtgggtctc gacccatttc cactcgccgg tctgagcgcg cacgcggtac tgcacgcgca
     1201 ggaagggccg ctccccgctc aaggtctcgt ccaccgcctg ctgcaccagc ggctgctcgc
     1261 gcggatgcac cagctccagc aggtcgcgca cggtggtgat cgccgcgccg cggggcccgc
     1321 ccactagctg tccccattgc tcgctcaggt acacctcgcc gctgcgcagg tcccagtcga
     1381 gcagcacgtc gcccgaaccc tccagcgcca gctgcaggcg ctcctggctg cggcgcaggg
     1441 actcgccggc gcagcgctgg gcgctgacgt cctcgaccac gtgcacgaag ccgaggaggt
     1501 tgttgccgtc gcggatcggg gcgccgttga tgcgcgtcca cgccacggtg gcgtcggcgt
     1561 gcaggtagcg gcaatcgacg ccatacggcg ttcggctgcg ggtagcggcc tcgcgcgcca
     1621 gcagcacgcg cgtgcggtcc tccggatgca gcgtggacag atggcccgaa gcgcgcacct
     1681 ggtcggctcg cagccccgag atctgctcga agttggcatt ggcgaagata cagccgccgg
     1741 cggcgtcggt cacgtacacg cccagtggcg aagcgtcgct gatggcgcgg aagcgcgcct
     1801 cgctctcgcg cagcgcgcgc atgctcttgc gccgggcggt gatgtcggtg aaattgccgc
     1861 gcaccccgac gatgcgcccg tccgcgtcga gcgtgggatg ggcgtagatc tccaccctgc
     1921 agtgcagccc gtcgcggcgc aggcagcgcg ccggcatgcg gcaggaatag atctcgccgc
     1981 ggatcaggga ctgcagggct tcgagcaccg gcggacggtc cgccggatgg aggatgtcgg
     2041 cgaagggccg ggacagcacg tcatgcggcg agccgccggt cagcatgctc cagccggctc
     2101 ccatctgact gacgttgccg aagcgatcca tggcgaaccc gacctcgggc tcggcctgtt
     2161 cctgccacgg cagtacctcg tgcgtagtca tcggctgcct gtatccagcg aggagcgcag
     2221 ccgtcccggc ctgcggccgg gcgccctccc tgggcccagt gtacgcctag atttctcaca
     2281 aagccagcct acaacttcct gacgagaaaa ggattgccag ggaaaacctg cttgggccgc
     2341 ttcaggccgt tcccagggcg ggcggatggt ggccgggccg gtcgatgggg tgctccccga
     2401 gggtccggtg gtgctcgatc t
//
LOCUS       DASPGE010000038         6542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1181931, whole genome shotgun sequence.
ACCESSION   DASPGE010000038 DASPGE010000000
VERSION     DASPGE010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181931"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..2770
                     /gene="gyrA"
                     /locus_tag="VD791_01215"
     CDS             194..2770
                     /gene="gyrA"
                     /locus_tag="VD791_01215"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HXV06623.1"
                     /translation="MEPFAKETIPVSLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMHESNNVWNRAYVKCARVVGEVLGKYHPHGDTATYEALVRMAQDFSMRYLL
                     VDGQGNFGSVDGDNAAAYRYTECRLERLAGELLADIDRETVDFVDNYDGKEREPTVLP
                     SRIPNLLLNGSSGIAVGMATNIPPHNLTEVVDACLKLLHEPDSAIEELIKLMPAPDFP
                     TAGIIYGIGGVHEGYRTGRGRVVMRARTHVEELDRGARSAIVIDELPYQVNKANLLMR
                     IGELVREKKLEGIAEIRDESDKSGMRVVIELKRGEVPEVVLNNLYKDTQMQDTFGMNM
                     VALVDGQPRVLNLKELLEAFLRHRREVVTRRTAYDLRQARNRGHILEGLAVALSNVDE
                     IIALIKESPSPAEARLRLMGRPWRSQLVTEMLARANAELTRPDGLVRELGLQADGGYR
                     LSEAQAQRILEMQLQRLTALERDKILNEYREVLDTIADLLDILAKPARITAIIAEELG
                     QVKEQFGDARRSEIQLNAQDLNLEDLIAPADVVVTLSHTGYIKSQPLSEYRAQKRGGR
                     GKQATTTKEDDFVEHLFIAHTHDYILCFSSRGRVYWIKVYEAPQGGRQSRGKPINNLV
                     PLEEGEKITAVLPIKTFDEAHYVFMATANGTVKKTPLSEFSRPRPSGIIAVSLDQGDR
                     LVGVAVTDGRHDVMLFSDAGKAVRFSEQDVRPMGREAHGVRGMTLNPGQQVISLVVAD
                     EEAKPVLVATENGFGKRTAVGEFTRHGRGSQGMIAIQTSARNGKVVAARLVAPDDEVM
                     LITTGGVLIRTRVSEIREMGRATQGVTLINLDQDEKLAGLERVVETDENGTDNGGESE
                     PG"
     gene            2894..3976
                     /gene="serC"
                     /locus_tag="VD791_01220"
     CDS             2894..3976
                     /gene="serC"
                     /locus_tag="VD791_01220"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HXV06624.1"
                     /translation="MRRVYNFSAGPGVLPEEVLRRAQTEMLDWHGSGMSVMEMSHRGE
                     EFIAIAEAAEADLRELLAVPAHYRVLFLQGGATSQFAMVPMNLLRGKTTADYVHTGEW
                     AKKAIAEARRYCTVNIAASSADRQFSYAPPQEAWKLTREAAYVHYTGNETIGGVEFHW
                     IPDTGEVPLVCDLSSSLLSRPLDVSRFGLIYAGAQKNIGPAGLTIVIVREDLLGRALP
                     FTPSMFDYRIQADNGSMYNTPPTYALYIAGLTFQWLKQRGGLGQMEKVNAAKAQLIYD
                     LLDGGGFYRSPVVRADRSRMNVPFTLRDATLDPAFLDAAGRRGLVQLKGHRSVGGMRA
                     SIYNAMPIEGVRALVEFMRDFEREHG"
     gene            3969..5156
                     /locus_tag="VD791_01225"
     CDS             3969..5156
                     /locus_tag="VD791_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HXV06625.1"
                     /translation="MADRLRILTLNAISHAGLKLLPPERYEVGNHLENPDAILLRSHD
                     MHGMHIPDTVRAVGRAGAGTNNIPVEALSRRGVPVFNAPGANANAVKELVLAGLFVAA
                     RNLLPAVRFVEGLNGSDEQLSRLVEDNKKHYAGIELPNRTLGVIGLGRVGSLVAEAAI
                     GLGMNVLGYDPDITVEAAWSLPSQVKKAHSIEEVLKTSEFVTLHVPLTPATRHLIDAE
                     RLALMRHGAVLLNFAREEVVDAEPVAAALAAKRLKYYVCDFPSARFSGNPAVAALPHL
                     GASTREAEDNCAVMVATEVRDYLEHGNIRNAVNFPDVVMQRESPYRVGIANANVPNML
                     GQISTAMAAAGLNIHNMLNKSKREMAYTLVDVDSPVGAPLVAALAAIQGVLSVRYLPA
                     QDR"
     gene            5202..6290
                     /gene="pheA"
                     /locus_tag="VD791_01230"
     CDS             5202..6290
                     /gene="pheA"
                     /locus_tag="VD791_01230"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HXV06626.1"
                     /translation="MGEENPVSGEDLNALRGRIDAIDERLLQLLNERADLARRIGELK
                     GDAPAYRPEREAQVLRRLTAANPGPLPGEAVERLFTEVISACRAVEAALSVAFLGPRG
                     TFSEEAAHKRFGGGAQGVPCASIDEVFRQVEAKQAGYGVVPVENSTEGAVGRTLDLLV
                     ATTARICGEVMLPVHQCFMSRAADAATVTRIYAHNQSLAQCHEWLGQHRPKAERVPVV
                     SNAEAARLASLEQGAACIGSRAAAALYSLNLLAENIEDNPNNTTRFAVIGHQAVPPSG
                     RDKTSLVMSATRNRPGAVHDLLAPLAAHGVNMSRLESRPSRTGMWEYVFFVDLEGHEQ
                     DAPVAAALEGVRSRAPFLKILGSYPAAE"
BASE COUNT         1136 a         2185 c         2280 g          941 t
ORIGIN      
        1 cttccaggac cgcgcccgcg ggctcgaccg gaatgaccca gcggggacag agaagggcgg
       61 tttcagccac ggatccaggg ccgattctaa ccgtgccgca tgataaaatt tggcgctgaa
      121 ttcgagcctg acgcagcgtg gttttcgacc tccggaacgg cccagaaatc ctcccagaag
      181 aagagccgaa ttgatggaac ccttcgccaa ggaaacgatc ccggtcagcc tcgaggagga
      241 gatgcggcgc tcgtacctcg attacgcgat gagcgtgatc gtgggccgcg cgcttcccga
      301 cgtccgcgac ggcctcaagc cggtgcaccg gcgggtgctc ttcgccatgc acgaatcgaa
      361 caacgtctgg aaccgggcct acgtgaagtg cgcgcgggtg gtcggcgagg tgctgggcaa
      421 gtaccacccg cacggcgaca ccgccaccta cgaggcgctg gtgcgcatgg cgcaggattt
      481 ctcgatgcgc tacctgctgg tcgacggcca gggcaacttc ggctcggtcg acggcgacaa
      541 cgccgccgcc taccgctaca ccgagtgccg cctggagcgg ctcgcgggcg agctgctcgc
      601 cgacatcgac agggagacgg tcgatttcgt cgacaactac gacggcaagg agcgcgagcc
      661 gacggtcctg ccctcgcgca tcccgaacct gctgctgaac ggctcctcgg ggatcgccgt
      721 cgggatggcg accaacatcc cgccgcacaa tctcaccgag gtggtcgatg cctgcctgaa
      781 gctgctgcac gagccggact ccgccatcga ggagctgatc aagctgatgc cggcgcccga
      841 cttcccgacc gccgggatca tctacggcat cggcggcgtg cacgaaggct atcgcaccgg
      901 ccgcggccgc gtggtgatgc gcgcgcgcac ccacgtcgag gagctggacc ggggcgcgcg
      961 ctcggcgatc gtcatcgacg agctgccgta ccaggtgaac aaagccaacc tgctgatgcg
     1021 catcggcgag ctggtgcgcg agaagaagct ggagggcatc gccgagatcc gcgacgaatc
     1081 ggacaagtcg ggcatgcgcg tggtgatcga gctgaagcgc ggcgaggtcc ccgaggtggt
     1141 cctgaacaat ctgtacaaag acacgcagat gcaggacacc ttcggcatga acatggtcgc
     1201 cctggtggac ggccagccgc gggtgctcaa cctcaaggag ctgctggagg ccttcctgcg
     1261 ccaccggcgc gaggtggtca cgcgccgcac cgcctacgac ctgcgccagg cgcgcaaccg
     1321 cggccacatc ctggaaggcc tggcggttgc cctgtccaac gtggacgaga tcatcgccct
     1381 gatcaaggag tcgccctcgc cggcggaggc acggctgcgc ctgatggggc gcccgtggcg
     1441 ctcgcagctg gtgaccgaga tgctggcgcg cgccaacgcc gagctaacgc gtcccgacgg
     1501 gctggtgcgc gagctcgggc tgcaggccga cggcggctac cgcctgtccg aggcgcaggc
     1561 gcagcgcatc ctggagatgc agctgcagcg cctgaccgcg ctggagcgcg acaagatcct
     1621 gaacgagtat cgcgaggtgc tggacacgat cgccgacctg ctcgacatcc tggccaaacc
     1681 tgcgcgcatc accgcgatca tcgccgagga gctcgggcag gtgaaggagc aattcggcga
     1741 tgcgcgccgc agcgagatcc agttgaacgc ccaggacctc aacctcgagg acttgatcgc
     1801 gccggccgac gtggtggtca ccctgtcgca caccggctac atcaagtccc agccgctgtc
     1861 cgagtatcgc gcgcagaagc gcggcggccg cggcaagcag gccaccacca ccaaggaaga
     1921 cgatttcgtc gagcacctgt tcatcgccca tacccacgac tacatcctgt gcttctccag
     1981 ccgcgggcgc gtgtactgga tcaaggtgta cgaggcgccc cagggcggcc gccagagccg
     2041 cggcaagccg atcaacaacc tggtgccgct ggaggagggc gagaagatca ccgcggtgct
     2101 gccgatcaag accttcgacg aggcgcacta cgtgttcatg gccaccgcca acggcacggt
     2161 gaagaagacg cccctgtccg agttttcgcg cccgcgcccg agcggcatca tcgccgtgag
     2221 cctggaccag ggcgaccgcc tggtgggcgt ggcggtcacc gacggccggc acgacgtgat
     2281 gctgttctcc gacgccggca aggcggtgcg cttctccgag caggacgtgc ggccgatggg
     2341 acgcgaggcg cacggcgtgc gcggcatgac gctgaatccg ggccagcagg tgatctcgct
     2401 ggtggtggcc gacgaagagg cgaagccggt gctggtggcc accgagaacg gcttcggcaa
     2461 gcgcactgcg gtgggcgagt tcacccgcca tggccgtggc agccagggca tgatcgcgat
     2521 ccagaccagc gcgcgcaacg gcaaagtggt cgccgcgcgc ctggtcgctc cggacgacga
     2581 ggtaatgctg atcaccaccg gcggggtgct gatccgcacg cgcgtgtccg agatccgcga
     2641 gatgggccgc gccacccagg gcgtgacgct gatcaacctg gaccaggacg agaagctggc
     2701 ggggttggag cgtgtggtgg aaaccgacga gaacggcacg gacaacggcg gcgagagcga
     2761 gccgggctga gagtcgatcg gcggaccgct tgcctcgcgc cccgagcgcg catgcggcgg
     2821 cgtgagtcga gatcaaggta aaggggagct ggcagtggga gcgatcgaac aggcgaacgc
     2881 gggcgcggta gccgtgcgcc gcgtctataa cttcagtgcc ggcccgggcg tgctgccgga
     2941 ggaggtgctg cgccgcgcgc agaccgagat gctcgactgg cacggcagcg gcatgtcggt
     3001 gatggagatg agccaccgcg gcgaagagtt catcgccatc gctgaagccg ccgaggccga
     3061 tctgcgcgag ctgcttgccg tcccggcgca ctacagggtg ctgttcctgc agggcggcgc
     3121 caccagccag ttcgccatgg tgccgatgaa cctgctgcgc ggaaaaacca ccgccgacta
     3181 cgttcacacc ggggaatggg ccaagaaggc gatcgccgag gccaggcgct actgcacggt
     3241 caacatcgcc gccagcagcg cagaccgcca gttcagctac gcgccgccgc aggaggcctg
     3301 gaagctcacg cgcgaggcgg cctacgtgca ctacaccggc aacgagacca tcggcggcgt
     3361 cgagttccac tggatcccgg acaccgggga ggtgccgctg gtgtgcgatc tgtcctccag
     3421 cctgctgtcg cgtccgctgg acgtgtcgcg ctttggcctg atctacgccg gcgcgcagaa
     3481 gaacatcgga cccgccgggc tgaccatcgt catcgtgcgc gaggacctgc tcggccgggc
     3541 gctgccgttc accccctcga tgttcgacta ccgcatccag gcggacaacg gctcgatgta
     3601 caacacgccg ccgacctatg cgctgtacat cgccgggctg acgttccagt ggctcaagca
     3661 gcgcggcggg ctggggcaga tggagaaggt gaacgcggcc aaggctcagc tgatatacga
     3721 cctgctcgac ggcggcggct tctaccgcag cccggtggtg cgcgcggatc gctcgcgcat
     3781 gaacgtgccg ttcacattgc gcgacgcgac cctggacccg gcgttcctcg acgccgccgg
     3841 gcgccgcggc ctggtgcagc tcaagggcca ccgttcggtc gggggcatgc gcgcctcgat
     3901 ctacaacgcc atgccgatcg aaggcgtgcg cgccctggtc gaattcatgc gcgatttcga
     3961 gcgcgagcat ggctgaccgg ctgcgcatcc tgacgctcaa cgcgatctcg cacgccggac
     4021 tcaagctgct gccgcccgag cgctacgagg tcggcaacca tctggagaac ccggacgcga
     4081 tcctgctgcg ctcgcacgac atgcacggca tgcacatccc ggacacggtg cgggcggtgg
     4141 ggcgcgcggg cgcgggcacc aacaacatcc cggtggaagc gctcagccgg cgcggcgtgc
     4201 cggtgttcaa cgcccccggg gccaacgcca atgcggtgaa ggagctggtc ctggccggcc
     4261 tgttcgtcgc cgcccgaaac ctgctgccgg cggtgcgttt cgtcgagggc ttgaacggca
     4321 gcgacgagca gctgtcgcgc ctggtggagg acaacaagaa gcactacgcc ggcatcgagt
     4381 tgcccaaccg cacgctcggg gtgatcggcc tgggccgggt cggcagcctg gtggccgagg
     4441 ccgccatcgg cctgggcatg aacgtgctgg gctacgatcc cgacatcacc gtggaggccg
     4501 cctggagcct gccatcccag gtgaagaagg cgcacagcat cgaggaggtg ctcaagacca
     4561 gcgagttcgt cacgctgcac gtgccgctca cgcccgctac ccggcacctg atcgacgccg
     4621 agcgcctggc gttgatgcgc catggcgcgg tgctgctcaa cttcgcgcgc gaggaggtgg
     4681 tggacgcgga gcctgtggcc gccgcgctgg ctgccaagcg gctgaagtac tacgtgtgcg
     4741 atttcccgag cgcgcgcttc agcggcaatc cggcggtggc cgcgctaccg cacctgggcg
     4801 cgtccacgcg cgaggcggag gacaactgcg ccgtgatggt cgccaccgag gtgcgcgatt
     4861 acctggagca cggcaacata cgcaacgcgg tgaatttccc ggacgtggtg atgcagcgcg
     4921 agtcgcccta ccgcgtgggc atcgccaacg ccaacgtgcc caatatgctg gggcagatct
     4981 ccaccgcgat ggcggccgcc ggcctcaaca tccacaatat gctgaacaag tccaagcgcg
     5041 agatggccta taccctggtg gacgtggaca gcccggtcgg ggcgcccctg gtggccgcgc
     5101 tggcggcgat ccagggcgtg ctgtcggtgc gctacctgcc ggcccaggac aggtgaggag
     5161 agcccggtgg gtgaggagag cccggtgggt gaggagagcc ggtgggtgag gagaacccgg
     5221 tgagcgggga ggacctgaac gcgctgcgcg gccgcatcga tgccatcgac gagcgcctgc
     5281 tgcagctgct caacgagcgc gccgacttgg cgcgccgcat cggcgagctc aaaggcgacg
     5341 ctccggcgta tcggccggag cgcgaggcgc aggtgttgcg gcggctcacc gccgccaacc
     5401 cgggaccgct tccgggcgaa gcggtcgagc gcctgttcac cgaagtcatc tccgcctgcc
     5461 gggcggtgga agcggccctg tcggtggcct ttctcggccc gcgcggcacc ttcagcgagg
     5521 aggccgcgca caagcgcttc ggcgggggtg cgcagggcgt gccctgcgcc tccatcgacg
     5581 aggtgttccg ccaggtcgag gcgaagcagg cgggctacgg ggtagtgccg gtggagaaca
     5641 gcaccgaagg ggcggtgggg cgcacgctcg acctgctggt cgccaccacc gcgcgcatct
     5701 gcggcgaggt gatgctgccc gtgcaccagt gcttcatgag ccgcgccgcg gacgccgcca
     5761 cggtgacccg catctacgcg cacaatcaga gcctggcgca gtgccatgaa tggctcggcc
     5821 agcaccggcc caaggccgag cgcgtgccgg tggtgagcaa cgcggaggcc gcgcggctgg
     5881 cgagcctgga gcagggcgcc gcctgcatcg gcagccgggc agccgcggcg ctgtactccc
     5941 tgaacctgtt ggccgagaac atcgaggaca acccgaacaa caccacgcgc ttcgccgtga
     6001 tcggccatca agcggtacct ccgtccggcc gcgacaagac ctcgctggtg atgtcggcga
     6061 cgcgcaaccg gccgggcgcg gtgcacgacc tgctggcgcc gctcgccgcg cacggcgtga
     6121 acatgtcgcg cctcgaatcg cgcccctcgc gcaccggcat gtgggagtac gtgttcttcg
     6181 tcgacctgga aggccacgag caggatgcac cggtggccgc cgccctggag ggcgtgcgca
     6241 gtcgcgctcc gttcctcaag atcctcggtt cgtatcccgc cgcggagtga cggctgtgat
     6301 tcgtgattcg tgactcgtga ttcgtttcta ggccttgagt tctgcccttc gcttatcgcc
     6361 actactgcta gagcagacaa tgcttgatca agcgcggccc gccaatcacg aatcgcgatt
     6421 cacgaatcac ggtagtttac gaatcacgaa tcacgaatca cgggtcgcca tgatcgacgc
     6481 ctgcgagctc acaccgtcct acgtccgcgg catcgccgcc taccagccgg gcaagccggt
     6541 tt
//
LOCUS       DASPGE010000039         3566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1299301, whole genome shotgun sequence.
ACCESSION   DASPGE010000039 DASPGE010000000
VERSION     DASPGE010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299301"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VD791_01235"
     CDS             complement(<1..281)
                     /locus_tag="VD791_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HXV06627.1"
                     /translation="MSIDYSEKIPNNVGLMDDQRVLKALEQWHPGYIDWWMTMGPEGF
                     QTSDVYLRTAVSIDPKGWAKFDYVKMPEYRWGILLAPQVEGRTIPFGEHL"
     gene            complement(283..1956)
                     /gene="boxC"
                     /locus_tag="VD791_01240"
     CDS             complement(283..1956)
                     /gene="boxC"
                     /locus_tag="VD791_01240"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HXV06628.1"
                     /translation="MNAPSPTPPSPAIDYRTSPDRYRHWRLAFEGAVARLTLDVQEDG
                     GLRPGYKLKLNSYDLGVDIELHDALQRIRFEHPEVRAVVVSSGKERIFCSGANIFMLG
                     LSTHAWKVNFCKFTNETRNGIEDASAHSGLKFLAACNGTTAGGGYELALACDEIVLVD
                     DRSSAVSLPEVPLLGVLPGTGGLTRLTDKRRVRHDLADIFCTTSEGVRGQRAKDWRLV
                     DEVVKPQQFAEHVARRAQQLAAASDRPPDAKGVALTPVERSDDTSGYRYEYVDVRLDA
                     RARQATITVRAPDAAVPGEIDAILARGASWWPLQMARELDDAILTLRTNHLDVGTWLL
                     KTAGDGERVLAADANLARHRDHWFVRETIGMLRRTLARIEVSSRTVMALIEPGSCFAG
                     TLLELALAADRSYMLDLPDDASAPAIALSEMNFGAYPRADRQSRIAARFYAETAPLAA
                     ARASLGRALRAAEAAELGLVTAAIDELDWVDEIRIVLEERASLSPDALTGMEANLRFG
                     AGETMETRIFARLSAWQNWIFNRPNAVGERGALKVYGTGNKAQFDWERV"
     gene            complement(2012..2899)
                     /locus_tag="VD791_01245"
     CDS             complement(2012..2899)
                     /locus_tag="VD791_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXV06629.1"
                     /translation="MPAQLARSEAPAADDAVFLSALGRRVRERRERLGMTRKLLAQQA
                     EVSERYLAQLESGDGNVSIVLLRRIAAALNLTPAALVDGEAADSVERRLVQRLLEGVP
                     AHRLEDVIFRLMRDFGQEEAARRKRIALIGLRGAGKSTLGVLLSRALDAPFVELNREI
                     QRETRLPVSEVFNLYGQAGYRRIEHRCLERVLAEHDSAVISTGGGLVSDEETFKLLLA
                     NCYTVWLKARPEEHMSRVLAQGDFRPMAGHQDAMDDLRRILAAREPLYRRADALVDTS
                     GQTPEESLRALRSLVNGPR"
     gene            3082..>3566
                     /locus_tag="VD791_01250"
     CDS             3082..>3566
                     /locus_tag="VD791_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXV06630.1"
                     /translation="MQRSTERMEDEVPELSHADHSVIPPRITIPREYNAAHDLLERNL
                     RAGRAAKPAFIDDAGSLTYGALAQRVDAFAHGLKALGLRMEDRVLLCLHDTVDFPTAF
                     LGAIKAGIVPIAANTLLTAADYEYMLRDSRARALIVSAPLLATFAPLLPRLPFLEHVI
                     VS"
BASE COUNT          546 a         1238 c         1188 g          594 t
ORIGIN      
        1 aggtgctcgc cgaacgggat ggtgcgcccc tccacctgcg gcgccagcag gatgccccag
       61 cggtattccg gcatcttgac gtagtcgaac ttggcccagc ccttgggatc gatgctgacc
      121 gcagtgcgca gatagacgtc ggaggtctgg aatccctccg ggcccatggt catccaccag
      181 tcgatgtagc cgggatgcca ttgctccagc gctttcaaga cgcgctggtc gtccatcagc
      241 ccgacgttgt tggggatctt ttcgctgtaa tcgatgctca tttcacaccc gctcccagtc
      301 gaactgcgcc ttgttgccgg tgccgtacac cttcagcgcg ccgcgctcgc cgaccgcgtt
      361 cggccggttg aagatccagt tctgccaggc ggacaggcgc gcgaagatgc gtgtttccat
      421 ggtttccccg gcgccgaagc gcaggttcgc ctccatgccg gtgagcgcat cgggcgacag
      481 gctggcgcgc tcctccagca cgatgcggat ctcgtcgacc cagtccagct cgtcgatcgc
      541 ggcggtgacc aaccccagct cggcagcctc cgccgcgcgc agcgcgcggc cgagtgacgc
      601 gcgtgcggcg gccagcggag ccgtctcggc gtagaaccgc gcggcgatgc gcgattgacg
      661 gtcggcgcgc ggataggcgc cgaagttcat ctccgacagc gcgatcgcag gcgcagacgc
      721 gtcgtccggg aggtcgagca tgtagctgcg gtcggccgcc agcgccagct cgagcagcgt
      781 cccggcgaag cacgagccgg gctcgatcag cgccatcacc gtgcgcgagc tgacttcgat
      841 gcgggccaac gtccgccgca gcatgccgat cgtttcgcgc acgaaccagt gatcccggtg
      901 gcgcgcgagg ttggcgtcgg cggcgagcac gcgttcgccg tcgcccgcgg tcttgagcaa
      961 ccaggtgccg acgtcgagat ggttcgtgcg cagcgtcagg atggcgtcgt ccagctcgcg
     1021 cgccatctgc agcggccacc aggacgcgcc ccgtgccagg atggcgtcga tctctccggg
     1081 cacggcggcg tcgggcgcgc gcaccgtgat cgtggcctga cgcgcacgcg catcgaggcg
     1141 tacgtctacg tactcgtatc gatagccgga cgtgtcgtcg ctgcgctcaa ccggcgtgag
     1201 cgccacgcct ttcgcatccg gcgggcgatc gctagccgcg gcgagctgtt gcgcacgccg
     1261 ggcaacgtgc tcggcgaact gctgcggctt caccacctcg tccaccagcc gccagtcctt
     1321 ggcgcgctgg ccacgcaccc cttcgctggt ggtgcagaag atgtcggcca agtcgtggcg
     1381 cacgcggcgc ttgtcggtca ggcgcgtgag cccgcccgtc cccggcagca cgcccagcag
     1441 cggcacctcc ggcagactca ccgccgagga gcggtcgtcc accagcacga tctcgtcgca
     1501 ggcgagcgcc agctcgtagc cgccgccggc ggtggtgccg ttgcacgcgg cgaggaactt
     1561 caggcccgag tgcgcgctgg cgtcctcgat accgttgcgc gtctcgttgg tgaacttgca
     1621 gaagttgacc ttccaggcgt gcgtcgacag ccccagcatg aagatgttcg cgccggagca
     1681 gaagatgcgc tccttgccgc tgctcacgac aacggcgcgc acctcgggat gctcgaagcg
     1741 gatgcgctgc agcgcatcgt gcagctcgat gtccacgccc aggtcgtagg agttgagctt
     1801 gagcttgtag cccggacgca ggcccccgtc ctcctgcacg tccagagtca accgcgccac
     1861 cgccccctcg aacgcgaggc gccagtgccg gtagcgatcc gggctggtgc ggtaatcgat
     1921 cgccggcgac ggtggcgtgg gagaaggggc gttcattctc ggtacctctc ttgtcctttt
     1981 cgtccccgga ttccgctgcg ctgcatccgg gctatctcgg gccgttcacg aggctgcgca
     2041 acgcgcgcag gctctcctcg ggcgtctggc cggaggtgtc caccagcgca tcggcccggc
     2101 ggtacagcgg ctcgcgcgcg gccagtatgc gcctgaggtc gtccatcgcg tcctggtgtc
     2161 cggccatcgg ccggaagtcg ccctgcgcga gcacgcgcga catgtgctcc tcgggccgcg
     2221 ccttgagcca aacggtgtag cagttcgcca gcagcagctt gaaggtctcc tcgtcggaga
     2281 ccagcccgcc gccggtcgag atcaccgcgc tgtcgtgctc ggccagcacg cgctcgaggc
     2341 agcggtgctc gatgcgccgg tagccggcct gaccgtacag attgaacacc tcgctcaccg
     2401 gcaggcgcgt ctcgcgctgg atctcgcggt tcagctccac gaacggggcg tcgagcgcgc
     2461 gcgacagcag cacgcccagc gtgctcttgc cggcgccgcg caggccgatc agcgcgatgc
     2521 gcttgcgccg cgccgcctcc tcctggccga agtcgcgcat gaggcggaag atgacgtctt
     2581 cgagccgatg cgcgggcacc ccttcgagca ggcgctgcac caggcgccgc tccacggagt
     2641 cggcggcctc gccgtcgacg agcgcggccg gcgtgagatt cagcgccgca gcgatgcgcc
     2701 gcagcagcac gatcgacacg ttgccgtcgc cggattcgag ctgggcgaga tagcgctcgg
     2761 agacttccgc ctgctgcgcc agcagcttgc gcgtcatgcc gagccgttcg cggcgctcgc
     2821 gcacacggcg gcccagggcg gacagaaaaa cggcgtcgtc ggccgccggc gcttcggagc
     2881 gggcgagttg tgcgggcata gagtttacct tgggcagtgc cgggaagtat gatgcgatcg
     2941 tagcgattct gcaagcatta tcttgcatgg acagggttcg ttgaactccc gctggcagcc
     3001 atttagacta gaaatatggc tatgatacaa acactcattt gatatgcaaa ataatgcatt
     3061 cactgcattg agcgctaccc ggtgcagcga tcgacggaaa ggatggagga cgaggtgccc
     3121 gaactcagcc atgccgatca cagcgtgatt ccaccgcgga tcaccatccc gcgcgagtac
     3181 aacgccgccc acgatctgct cgagcgcaac ttgcgcgcgg ggcgcgccgc caagccggcg
     3241 ttcatcgatg acgcaggcag cctcacctac ggtgcgctcg cgcagcgcgt ggacgccttc
     3301 gcgcatggct tgaaggcgct ggggctgcgg atggaagacc gcgtgctgct gtgcctgcac
     3361 gacaccgtgg atttccccac tgcatttctg ggcgccatca aggccggcat cgtcccgatc
     3421 gcagccaaca cgctgctcac cgccgcggac tacgaataca tgttgcgcga cagccgtgcg
     3481 cgcgccctga tcgtgtcggc gccgctgctc gcgaccttcg cgcccctgct cccgcgcctg
     3541 ccgttcctcg agcacgtgat cgtgtc
//
LOCUS       DASPGE010000040         6596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_11439, whole genome shotgun sequence.
ACCESSION   DASPGE010000040 DASPGE010000000
VERSION     DASPGE010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11439"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1479)
                     /locus_tag="VD791_01255"
     CDS             complement(40..1479)
                     /locus_tag="VD791_01255"
                     /inference="COORDINATES: protein motif:HMM:NF017870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HXV06631.1"
                     /translation="MRKLKIALALVLFFIVGAAAAFPWYLGRQAEQRFLAGLKHPALG
                     DNSALAVSLVQYERGWLNSSAVHRVALKADPDVYFDVHHAIRHLPDPERGLVMVHSTP
                     RWPQKVQAAADYYFGNQPALSVHTVVDFDRNVSMTLESPAFSKPMLTRPEVKLTWGGG
                     SGRISMTGPARVAVLFDLPRVQVDGSDLSATFADMKVQGDWDVTGNQAEWSGQTSMGI
                     GELSLASPFGSASLKGVDSSVVQRNQGATVLVGYTLKVREGVTAAAGEQAQGFRDAVL
                     EIELDRLDKQVLAKYFDDVADAERAAVSPQAHKRLAAQLAMGMMSELLKGSPEVRVKK
                     LGMTMAKGSLSGSAVLGFDGNGMGQGGAPAEMLSRVKFSGSAELSTTLLQAWLEKQAR
                     AQASNALTAQGTPPEEARVHALTQQIAQQTLAGMEASGLLKAEGDKFVVRAELTAGSL
                     SINGMAGDQFLPGLLPAMTPSAAGEDEKA"
     gene            1602..2477
                     /locus_tag="VD791_01260"
     CDS             1602..2477
                     /locus_tag="VD791_01260"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HXV06632.1"
                     /translation="MAGAFVHPEYLVSTGWLAQHLDDPRLRVLDATTHLPPLPDFSLY
                     EVVSGRADFDKAHIPGAQFVDIEHELSTPQPRLHFTLPDAQTFADAMGRLGVGDDTRV
                     ICYASANHWWATRLWWMLRVFGHDQAAVLDGGFQKWRAEGRPVESGPGRARAPARFTP
                     RRRAELVAGKDEVLAAIGRRRVCTVNALRPEQHAGTGGVHYGRRGHIPGSVNIAAVAH
                     VNPDNTFKSADELRTMFAGALASPEVITYCGGGIAATSVALVLAALGYDRVKVYDNSL
                     TEWAADPQLPMATGA"
     gene            2491..3462
                     /locus_tag="VD791_01265"
     CDS             2491..3462
                     /locus_tag="VD791_01265"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV06633.1"
                     /translation="MLEHFPVHRGDYDGPVLITGAGGCIGSWALALLARAGVPAVAFD
                     LNEDKRRPRLLMSDAQLAAVRWLTSDIADFDAVLKAVEASGATSIVHLAALQVPFCKA
                     DPVAGAKVNVVGTVNVLEAARKLGIRRLSYASSIAAHGALEGHGTLPTLYGAYKYCNE
                     ETARVYSQDCGVHSVGLRPGVVYGVGRDQGMTSKTTVALLAAAAGKPYDIPFRGPISW
                     LHAGEVASAFLKAVSQPRARAAVFDINGVASTVEASVALLQRVAPGAAITCSGQALPF
                     PMNLSDAPVRAYLGDYGSVPVEAGMRGTLEAFKGLLASGLLDAKSIA"
     gene            3459..3962
                     /locus_tag="VD791_01270"
     CDS             3459..3962
                     /locus_tag="VD791_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXV06634.1"
                     /translation="MRGAIAARVALLSVVLLLCVAPLARAAPQIDEPAPPLKGRLFSG
                     SDFDLATLRGKVVLVNFYSSYCKRCAFEIGMLEAFYEEHRAEGFEVVAIGVDDLRDRH
                     RVERMLGIYNLPGMMADELTESGFAPRYPTPTAFIVDRDGILRHRITGGKIPAHYREL
                     VLPLLTR"
     gene            4078..4863
                     /locus_tag="VD791_01275"
     CDS             4078..4863
                     /locus_tag="VD791_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06635.1"
                     /translation="MAAALLLGLPCLAQAATPSFLCSKARTWVEKTICASERLSDLDL
                     ELATVYARLLRVSTGNAEKVLTSDQHRWWATRDECRRQVRPGECLETRYVQRIALLKA
                     RPDYTELRPGAVELPPESLSAVGEGWSKSLSRYLKAMRACLRRAPAAVKVIGAGWEEP
                     GEEPNVGLRMRGPRDEIWVCTAARNGSRVLSFHEANIYEPLPPEGPLFYPDPTAPPAD
                     ACGTPVQVLDEYEAPVGWLGPQCAQTATQTPAETPSGTPPGTQ"
     gene            5026..5460
                     /locus_tag="VD791_01280"
     CDS             5026..5460
                     /locus_tag="VD791_01280"
                     /inference="COORDINATES: protein motif:HMM:NF016211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HXV06636.1"
                     /translation="MTRHDYSDEVRKHGSLAVRVLLVLAGTAFVALGIAGAFLPVLPT
                     TPFLLLAAACYARASTRFYNGLLNSRMFGPTIREWQRYRSIRYRTKLTAIALMAVTLS
                     ISIAFFVTPPALQAALALLGLALAVYLYRIPSRDHPRRRAGS"
     gene            5893..>6596
                     /locus_tag="VD791_01285"
     CDS             5893..>6596
                     /locus_tag="VD791_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV06637.1"
                     /translation="MNPPHPSQSLRRDAQIIALVGLAHGTSHFYHLLFAPLFPWLKAA
                     FGLSYAELGLLLSAFFVVSGIGQALAGFVVDRVGAWIVLMGGVGLLGVAALTLAASQS
                     YPMLVLAAAIAGLGNSVFHPADFTILNRRVSVPRLGHAFGMHGILGTMGWATAPVFLA
                     GLATLIGWRLALLAAALLTFAILALLLANRALLDTRELHAAAAKGAVQADALAFLRLP
                     GVWMCFAFFFITSMSLG"
BASE COUNT          935 a         2315 c         2253 g         1093 t
ORIGIN      
        1 cacgccttcc ggtcacgcct ttcgatcacg cagtcccgat cacgccttct cgtcttcccc
       61 cgccgcactc ggcgtcatcg ccggcagcaa tccaggcagg aactgatccc cggccatgcc
      121 gttgatcgac aagctgcccg ccgtgagctc ggcgcgcacc acgaacttgt cgccctcggc
      181 cttgagcagg ccgctcgcct ccatcccggc gagggtttgc tgcgcgatct gctgcgtgag
      241 cgcatgcacg cgcgcctcct cgggcggcgt gccctgcgcg gtcagcgcat tgctcgcctg
      301 tgcacgcgcc tgcttctcga gccaggcctg cagcagcgtg gtggacagct cggccgaacc
      361 gctgaacttc acccgcgaca gcatctcggc gggcgcgccg ccctggccca tgccgttgcc
      421 gtcgaagccc agcaccgccg aacccgacag actgcccttc gccatggtca tgccgagctt
      481 cttcacgcgc acctcgggcg aacccttgag cagctcgctc atcatgccca tcgccagctg
      541 cgccgccagg cgcttgtgcg cctgcgggct caccgcggcg cgctcggcgt cggccacgtc
      601 gtcgaagtac ttggccagca cctgcttgtc gaggcggtcg agctcgatct ccagcaccgc
      661 gtcccggaaa ccctgcgcct gctcgccggc ggcagcggtc acgccttcgc gcaccttcaa
      721 ggtgtagccc accagcacgg tcgcaccctg gttgcgctgg accacgctgc tgtccacgcc
      781 tttgaggctg gccgacccga aggggctggc caacgacagc tcgccgatgc ccatgctcgt
      841 ctggccggac cattccgcct ggttgccggt gacatcccag tcgccctgca ccttcatgtc
      901 ggcgaaggtc gccgacagat cgctgccgtc gacctgcacg cgcggcagat cgaacaggac
      961 cgcaacgcgc gcggggccgg tcatcgagat ccggccgctg cctccgcccc aggtcagctt
     1021 gacctcgggc cgggtgagca tcggcttgct gaacgcgggc gactccagcg tcatgctgac
     1081 gttgcggtcg aagtccacca cggtgtgcac cgacagcgcc ggctggttgc cgaagtagta
     1141 gtcggcggcg gcctgcacct tctgcggcca gcgtggcgtg gagtgcacca tcaccagacc
     1201 gcgctcggga tcgggcaaat gacggatggc gtgatgcacg tcgaagtaga cgtcggggtc
     1261 cgccttgagg gcgacgcgat gcaccgcgga cgagttcagc cagccgcgct cgtattgcac
     1321 cagcgacacg gccagcgccg agttgtcgcc cagcgcggga tgcttcagcc ctgcgagaaa
     1381 gcgctgctcg gcctgccggc ccaggtacca ggggaaagca gcggcggcgc cgacgatgaa
     1441 aaacaagacg agggccagcg cgatcttgag ttttcgcatg gatgctcccg gtttgacact
     1501 tcgctgtcta tcggcgcctg tacgacgcag catccgtgcc cgggtgcggc gcgtcgcgac
     1561 cgtgctacca tcgccgcgtt cccgttgacg cggaggcgac gatggcaggc gcgttcgtgc
     1621 accccgagta cctggtctcc accggctggc tggcgcagca tctggacgac ccgcgcctgc
     1681 gggtgctgga cgcgaccacg catctgccgc cgttgccgga cttctcgctg tacgaggtgg
     1741 tgtcgggccg ggcggatttt gacaaggcgc acattcccgg tgcgcagttc gtcgacatcg
     1801 agcacgagct gtccacgccg cagccacgcc tgcacttcac gctgcccgat gcgcagacgt
     1861 tcgccgacgc gatgggacgg ctgggggtgg gcgatgacac ccgcgtgatc tgctacgcga
     1921 gcgccaacca ctggtgggcc acgcgcctgt ggtggatgct gcgggtcttc ggacacgatc
     1981 aggccgcggt gctcgatggc ggatttcaga agtggcgcgc agaggggcgg ccggtcgaga
     2041 gcggaccggg acgggcgcgt gcgccggcac ggttcacgcc gcgccggcgc gcggagctgg
     2101 tcgccggcaa ggacgaagtg ctcgccgcca tcggacggcg gagggtgtgc acggtgaacg
     2161 cgctgcgacc cgagcagcac gccgggaccg gcggcgtgca ttacggccgc cgcggtcata
     2221 tcccggggag cgtcaatatc gccgcggtcg cgcacgtcaa tcccgacaat accttcaaga
     2281 gcgcggacga gctgcgcacg atgttcgccg gcgcgctggc cagccccgag gtgatcacct
     2341 actgcggcgg cggcatcgcg gccaccagcg tggcgctggt gctcgccgcg ctgggctacg
     2401 accgcgtcaa ggtgtacgac aactcgctca ccgagtgggc ggccgatccg cagttgccga
     2461 tggccaccgg ggcctagcag gaggaagatc atgctcgagc atttccccgt ccaccgcggc
     2521 gactacgacg gcccggtgct gatcaccggg gccggcggct gcatcggcag ctgggcgctg
     2581 gcgttgctcg cgcgcgccgg cgtcccggcg gtggcgttcg atctgaacga ggacaagcgc
     2641 cgcccgcggc tgctgatgtc cgacgcgcag ctcgccgcgg tgcgctggct caccagcgat
     2701 atcgcggact tcgacgcggt cttgaaggcg gtcgaggcga gcggcgcaac ctcgatcgtg
     2761 catctggcgg ccctgcaggt gccgttctgc aaggccgatc cggtggccgg cgcgaaggtg
     2821 aacgtggtag gcacggtgaa cgtgctggag gccgcgcgca agctcggcat ccggcgcttg
     2881 agctacgcca gctcgatcgc ggcgcacgga gcgctggagg gccacggcac gctgccgacg
     2941 ctgtacggcg cctacaagta ctgcaacgag gagaccgcca gggtctattc ccaggactgc
     3001 ggcgtgcaca gcgtcgggct gcgcccgggg gtggtgtacg gtgtgggtcg cgatcagggt
     3061 atgacctcga agacgaccgt ggcgctgctg gcggctgcag ccggcaagcc ctacgacatt
     3121 ccgttccgcg gtccgatctc ctggctgcac gcgggcgaag tcgccagcgc tttcctgaaa
     3181 gccgtgtcgc agccgagagc gcgagccgcg gtgttcgaca tcaacggcgt ggcctcgacg
     3241 gtggaggcgt cggttgcgct gctgcaacgg gtggcgcccg gtgcggcgat cacctgctcc
     3301 ggccaggcgc tgcccttccc gatgaacttg tccgacgccc cagtgcgtgc ctatttgggc
     3361 gactacggtt cggtcccggt cgaggccggt atgcgcggca cgctggaggc gttcaagggg
     3421 ctgcttgcaa gcggcctgct ggacgcgaag agcatcgcat gagaggcgcc atcgccgccc
     3481 gtgtcgcgct tctaagtgtc gtgctgctgc tgtgcgtcgc gccgctggcg cgggccgcgc
     3541 cgcaaatcga cgagccggcg ccgccgctca aaggtcgcct gttctccggc tccgacttcg
     3601 acctcgccac cctgcgcggc aaggtggtgc tggtcaactt ctactccagc tactgcaagc
     3661 gctgcgcatt cgagatcggc atgctcgaag cgttctacga agagcaccgc gccgaaggtt
     3721 tcgaggtagt ggccatcggc gtggacgact tgcgcgatcg ccatcgggtc gaacgcatgc
     3781 tcggcatcta caatctgccg gggatgatgg cggacgagct caccgagagc ggcttcgccc
     3841 ctcgctaccc cacgcccacc gccttcatcg tcgaccgcga cggcatcctg cgccaccgca
     3901 tcacgggcgg caagatcccc gcgcattacc gggagctggt gctcccgctg ctgacgcgct
     3961 gaggcggggg cgcgccgggc gagagcgttc cggcatgcgt tgtgcatcgc ggtgcgctgc
     4021 caagccacct ggatcagctc tatgcgcctg tcccctgcaa acgacgtcct tcgcgctgtg
     4081 gccgcggccc tgctgcttgg ccttccctgc ttggcgcagg cggccacgcc cagcttcctg
     4141 tgcagcaaag cccgcacctg ggtggagaaa accatctgtg cgagcgagcg cttgtcggat
     4201 ctggatctgg agctggccac ggtgtatgcg cgtctgctca gggtcagcac cggcaacgcg
     4261 gagaaggtgc tcacttcgga tcagcaccgc tggtgggcaa cccgcgacga gtgccgacgc
     4321 caggtgcgtc cgggcgaatg cctggagacg cgctacgtgc agcgcatcgc gctgctcaaa
     4381 gcacggccgg actacactga gctgcggccc ggagcggtgg agctgccgcc cgagtctctg
     4441 tccgccgtcg gcgagggctg gtccaagtcg ctgtcgcgct atttgaaggc gatgcgcgcc
     4501 tgcctgcgcc gcgcgccggc ggcggtgaag gtgatcggtg cgggctggga ggagcccggg
     4561 gaggaaccca acgtcgggtt gcgcatgcgc ggtccgcgcg acgaaatctg ggtttgcacc
     4621 gccgcccgca acggttcgcg agtgctgagc tttcacgagg ccaacatcta cgaaccgctg
     4681 ccgccggagg gaccgctgtt ctaccccgac ccgaccgcac cgccggcaga cgcgtgcggt
     4741 acaccggtgc aggttctgga cgagtacgaa gcgcccgtgg ggtggctggg gccgcagtgc
     4801 gcgcagaccg cgacgcagac gcccgccgag acgcccagcg ggacgccccc cgggacgcag
     4861 tgattcgaga gccggattcg cgccgctgac gcggcttcat ccgggctacg gccgagcgca
     4921 gcgatctcgt gtcgcccgca ataccgcggc gcatgcagcg cccgcatccg catctccgtg
     4981 tagcatgcgt taccgcagcc cgcagcgcac ggatttgttc gcgcaatgac ccgccacgac
     5041 tacagcgacg aggtgcgcaa gcacggctcg ctcgcggtgc gcgtgctgct ggtgctcgcc
     5101 ggcaccgcgt tcgtcgcgct gggcatcgcc ggggcgttcc tgccggtgct gcccaccacc
     5161 ccgttcctcc tgctcgccgc ggcttgctat gcgcgcgcgt cgacccgctt ctacaacggt
     5221 ctgctcaaca gccgcatgtt cggcccgacc atccgcgagt ggcagcgcta tcgcagcatc
     5281 cgctaccgca ccaagctcac cgccatcgcg ctgatggcgg tcacgctgtc gatatcgatc
     5341 gccttcttcg tcacgccgcc ggcgttgcag gcggcccttg cgctgctcgg cctggcgctc
     5401 gccgtctacc tgtaccgaat cccgtcccga gatcatcctc ggcggcgggc cggatcgtga
     5461 aacaacgcaa cggcggcgat cgcgtccggg gcattgcgcc gattgacgca ccatgacatc
     5521 ggcgtcgccg gcgcgggaca gcgacgtgcc gcgcgacgcc ccgctgggcc gggttcaggc
     5581 ggtatgaatc agggcgttat gtgccggtcc gagatcccgc aggcgaccgg cgcagttcct
     5641 gcttcccagc gttgcgcgtt cgtccaaccc ccctgccatg cgccaactcg gatcccaccc
     5701 ctttgtgcac agcctgttcc gcgccggcct gccctggctg ctggtcgcgg tgctgatgct
     5761 gggcaacctg atcctgtccc agaccagcgc ggtgctgcac tgacccgccc cgctggcgga
     5821 tcgggtattt cgaaccgcgg cggaccgatt tgaccgggcc gcgcgcatgc cttaaggttc
     5881 ggggtccgac ccatgaaccc cccgcacccg agccagtcgc tgcgccgcga cgcccagatc
     5941 atcgccctgg tcggactggc tcacggcact tcgcacttct atcacctgct gttcgcaccc
     6001 ctgtttccgt ggctcaaggc ggccttcggg ctgagctacg cggaactggg gctgttgctg
     6061 agcgccttct tcgtggtatc cggcatcggc caggcgctgg cgggcttcgt ggtcgatcgc
     6121 gtcggcgcct ggatcgtact gatgggcggg gtgggtttgc tgggggtcgc cgcgctgacc
     6181 ctggcggcca gccagagcta cccgatgctg gtgctggcgg cggcgattgc cggcctgggc
     6241 aacagcgtgt tccatccggc cgatttcacc atcctcaacc gtcgcgtcag cgtgccccgg
     6301 ctcggtcacg cgttcggcat gcacggcatc ctgggcacaa tgggctgggc caccgcgccg
     6361 gtgttcctgg ccgggctggc cacgctgatc ggctggcggc tggcactgct ggccgcggcg
     6421 ctgctcactt tcgcgatcct ggcgctgctg ctggccaatc gcgcgctgct ggacacgcgc
     6481 gagctgcatg cggcggccgc gaagggcgcg gtgcaggcgg acgcgctcgc attcctgcgc
     6541 ctgccgggcg tgtggatgtg cttcgccttc ttcttcatca cctccatgtc gctggg
//
LOCUS       DASPGE010000041         3955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1299675, whole genome shotgun sequence.
ACCESSION   DASPGE010000041 DASPGE010000000
VERSION     DASPGE010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299675"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..124)
                     /locus_tag="VD791_01290"
     tRNA            complement(33..124)
                     /locus_tag="VD791_01290"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(88..90),aa:Ser,seq:cga)
     gene            232..1656
                     /gene="mltF"
                     /locus_tag="VD791_01295"
     CDS             232..1656
                     /gene="mltF"
                     /locus_tag="VD791_01295"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HXV06638.1"
                     /translation="MRKLLLSLVLLLPLICDPGFIAERPVPSPSKTGELVVLTRNTPT
                     TRYIDSEGHYAGLEYDLIEMFAAELDLRVRYLDRLPLYQLAPALQRHEAHLAAAGLAI
                     TPERLDEFQFGPAYQMVQPVLAYNTHNPAPRRLGDMVGKRIEVVKGSSGVEQLRALRK
                     RLPRLRWKEVAESDSEGLLTRLSEGKTDLVVTESHVLDVVRRFYPNLARAFPIGDPTP
                     LAWMFPPDGDPVLAERAKEFFLRITYDGTLRHMLDRYYGHVQRLGPLDFANFLERRLT
                     VLPGYRAAFKEAQELTGIDWRLLAALGFQESHWDPLAVSPTGVRGLMMLTSETADRMR
                     VTDRLEPRQNILAGARYLLELKDTLPVRIREPDRTWLALAAYNVGYGHLEDGRILAQR
                     QGLNPDLWVNVKQVLPLLTRYEYYSTVKYGFCRGGEAVVLTENIRSYYDVLRRLEAPH
                     SPGFELFTRTVARPQAQPVPVASQ"
     gene            complement(1742..3028)
                     /gene="serS"
                     /locus_tag="VD791_01300"
     CDS             complement(1742..3028)
                     /gene="serS"
                     /locus_tag="VD791_01300"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HXV06639.1"
                     /translation="MLDIQLLRNDLEGVAARLATRGIPVPVAEFQRLEGVRKSVQTRT
                     QELQSRRNALSKLIGEAKRKGEDAAALMAEAAALPDEVKRVEDELERVQRELTDLLLV
                     IPNVPHASVPVGKSADDNVELRRVATPRGFDFPVKDHVDLGEALGMLDFQRATKISGS
                     RFTLITGQLARLHRAIAQFMLNVHTLEHGYTEVYAPYLVNADSMRGTGNLPKFEDDLF
                     PVPRRDADKLYLIPTAEVPVTNMVRDEILAAEALPLKFVCHSPCFRSEAGSYGKDTRG
                     MIRQHQFDKVEIVQIVRPEVSYVALEQLTGHAEEILRRLELPYRVVALCTADMGFSAA
                     KTYDIEVWLPGQNAYREISSCSNFEAFQARRMQARFRNEKGRPELVHTLNGSGLAVGR
                     TLVAILENCQRADGSVDVPAALHAYMGGVTRIEPAR"
     gene            complement(3033..>3955)
                     /locus_tag="VD791_01305"
     CDS             complement(3033..>3955)
                     /locus_tag="VD791_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination factor protein RarA"
                     /protein_id="HXV06640.1"
                     /translation="GLFTFIGATTENPSFEVNSALLSRAAVYVLKPLTEDELSALFER
                     AHAQHMPDLTFAAQARELAIGFADGDARRLYNVLEQVADAAATRGRTEIDEAFVRDAL
                     AERLRRFDKGGEEFYDQISALHKSVRGSAPDAALYWLTRMLDGGADPLYVGRRLVRMA
                     VEDIGLADPRALRLALDACETFERLGSPEGELALAQAAIYLACAPKSNAAYLAYNAAR
                     ALVSHDGSRPVPLHLRNAPTRLMKQLGYGRTYRYAHDEPDAYAAGEDYFPEGMPQVGF
                     YHPTPRGLEGKIGEKLAHLRELDAAAKKKS"
BASE COUNT          595 a         1398 c         1339 g          623 t
ORIGIN      
        1 accaccctcg acgccacgcg cgcagcgcgt gctggcggag agggtgggat tcgaacccac
       61 ggtcccccgc aagagggaca acagatttcg agtctgcccc gttcggccac tccggcacct
      121 ctccagcggc cgccattcta ccgcaagcgc cccatgccac ggcccgggcc ctgctaggat
      181 gccgccggac gcgtagcgcc gtgcgccttc ttcttgcttc cgacgatctc gatgcgcaag
      241 cttctgctgt cgctcgtctt gctgctgccg ctcatctgcg acccgggctt catcgcggag
      301 cggccggtgc cgtccccgtc gaagaccggc gagctggtgg tgctcacgcg caacacgccg
      361 accacgcgct acatcgactc cgaggggcac tacgccgggc tcgagtacga cctgatcgag
      421 atgttcgcgg ccgagctgga cctgcgcgtg cgctatctcg accggctgcc gctgtaccag
      481 ctggcgccgg cgctgcagcg ccacgaggcg cacctggccg ccgccgggct ggccatcacg
      541 ccggaacggc tcgatgagtt ccagttcggt ccggcctacc agatggtgca gccggtgttg
      601 gcctacaaca cccacaaccc ggcaccgcgc cgcctgggag acatggtcgg caagcgcatc
      661 gaggtggtga agggctcgtc cggcgtcgag cagctgcgcg cgctgcgcaa gcgccttccg
      721 cgcctgcgct ggaaggaagt cgccgaaagc gacagcgaag gcctgctgac ccgcctgtcg
      781 gagggcaaga ccgacttggt ggtaaccgaa tcgcacgtgc tcgacgtggt gcgcaggttc
      841 tacccgaatc tcgcgcgcgc cttcccgatc ggcgatccga cgccgctggc gtggatgttc
      901 ccgccggacg gcgatccggt gctggccgag cgcgccaagg aattcttcct gcgcatcacc
      961 tacgacggca cgctgcgtca catgctggac cgctactacg ggcacgtgca gcgcctcggc
     1021 ccgctcgact tcgccaactt cctggagcgc cgcctgacgg tgctgccggg ctaccgggcg
     1081 gcgttcaagg aggcgcagga gctcaccggc atcgactggc gactgctggc ggcgctcggt
     1141 ttccaggaat cgcactggga cccgctggcg gtgtcgccca ccggggtgcg cggcctgatg
     1201 atgctgacct cggagaccgc cgaccgcatg cgcgtcaccg atcggctgga accgcgccag
     1261 aacatcctgg ccggcgcgcg ctacctgctc gaattgaaag acacgctgcc ggtgcgcatc
     1321 cgcgagccgg accgcacctg gctggcgctg gcggcttaca acgtcggcta cggccacctg
     1381 gaggacgggc gtatcctggc gcagcgccaa ggcctgaatc cggatctgtg ggtgaacgtc
     1441 aagcaagtgc tgccgctgct cacgcgctac gagtactaca gcacggtgaa gtacggcttc
     1501 tgccgcggcg gcgaggcggt ggtgctgacc gagaacatcc gcagctacta cgacgtcctg
     1561 cggcgcctgg aagcgccgca ctcgcccggc ttcgagctgt tcaccaggac cgtggcgcgc
     1621 ccgcaagcgc agccggtgcc ggtggccagc cagtgacatg caaggctgag caggcgcacc
     1681 agccagctcg gcaatacccc tcacccccgc cctctcccgc aacgggagag ggagaaagca
     1741 ttcagcgcgc cggctcgatg cgcgtcaccc cgcccatgta ggcgtgcagc gccgccggca
     1801 cgtccaccga gccgtcggcg cgctggcagt tctccaggat cgccaccagg gtgcggccta
     1861 cggcgaggcc cgagccgttc agcgtgtgca ccagttccgg tctgcccttc tcgttgcgga
     1921 agcgcgcctg catgcggcgc gcctggaacg cctcgaagtt gctgcacgag gagatctcgc
     1981 ggtaggcgtt ctgccccggc agccagacct cgatgtcgta ggtcttggcc gcggaaaaac
     2041 ccatgtccgc ggtgcacagc gctaccacgc ggtagggcaa ctcgagccgc cgcaggatct
     2101 cctccgcgtg gccggtgagc tgctcgagcg cgacgtagga aacctccggg cgcacgatct
     2161 gcacgatctc gaccttgtcg aactgatgct ggcggatcat gccgcgcgtg tccttgccgt
     2221 aggagccggc ctcgctgcga aagcacggcg agtgacagac gaacttgagc ggcagcgcct
     2281 ccgcggccag gatctcgtcg cgcaccatgt tggtgaccgg cacttccgcg gtggggatca
     2341 ggtacagctt gtcggcgtcc cggcgcggca ccgggaacag atcgtcctcg aacttgggca
     2401 ggttaccggt gccgcgcatg ctgtcggcgt tgaccaggta gggcgcgtac acctcggtgt
     2461 agccgtgctc cagggtgtgc acgttcagca tgaactgcgc gatggcgcgg tgcagccgcg
     2521 cgagctgccc ggtgatcagc gtgaagcgcg agccgctgat cttggtcgca cgctggaaat
     2581 cgagcatgcc cagcgcctcg cccagatcga cgtggtcctt gaccgggaaa tcgaagccgc
     2641 gcggcgtggc gactcgccgc agctcgacgt tgtcgtcggc ggacttgccc accggcaccg
     2701 acgcatgcgg cacgttgggg atgaccagca gcaggtcggt cagctcgcgc tgcacccgct
     2761 cgagctcgtc ctccacgcgc ttgacctcgt ccggcagggc ggcggcctcc gccatcagcg
     2821 ccgcggcgtc ctcgcccttg cgcttggcct cgccgatcag cttggacagc gcattgcgcc
     2881 ggctctgcag ctcctgggtg cgcgtctgca cgctcttgcg cacgccttcc aggcgctgga
     2941 actccgccac gggtacgggg atgccacgcg tggcgagccg cgccgcgacg ccctccagat
     3001 cgtttcgtag aagttgtatg tcgagcattg gctcagctct tcttcttcgc cgccgcgtcg
     3061 agctcgcgca ggtgcgcgag cttctccccg atcttgccct ccagacccct gggcgtggga
     3121 tggtagaagc cgacctgcgg catgccctcc gggaaatagt cctcgcccgc ggcatacgcg
     3181 tcgggctcgt cgtgggcgta gcgataggtc ctgccgtagc ccagctgctt catcagccgg
     3241 gtgggggcgt tgcgcaggtg cagcggcacc ggccgggagc cgtcgtgcga caccaacgcg
     3301 cgggcggcat tataagcgag gtacgcggcg ttgctcttcg gcgcgcatgc caggtagatg
     3361 gcggcctgcg ccagcgccag ctcgccctcc ggcgagccga gccgctcgaa ggtctcgcac
     3421 gcgtccagcg ccagccgcag cgcgcgcgga tcggccaggc cgatgtcctc gaccgccatg
     3481 cgcaccagcc tgcgtccgac gtagagcgga tcggcgccgc cgtcgagcat gcgcgtgagc
     3541 cagtacaacg ccgcgtcggg cgcggagccg cgcaccgact tgtgcagcgc cgagatctgg
     3601 tcgtagaact cctccccgcc cttgtcgaag cggcgcagcc gctcggcgag cgcgtcgcgc
     3661 acgaaggctt cgtcgatctc ggtacgcccc cgcgtggcgg cggcgtcggc cacctgctcc
     3721 agaacgttgt acaagcggcg cgcgtcgccg tcggcgaagc cgatcgccag ctcgcgcgcc
     3781 tgcgccgcga aggtgagatc cggcatgtgc tgcgcgtgcg cgcgctcgaa cagcgcgctc
     3841 agctcatcct cggtgagcgg cttgagcacg tacacggccg cgcgcgacag cagcgcgctg
     3901 ttcacctcga aggacgggtt ctcggtggtg gcgccgatga aggtgaacag gccct
//
LOCUS       DASPGE010000042        18565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1182360, whole genome shotgun sequence.
ACCESSION   DASPGE010000042 DASPGE010000000
VERSION     DASPGE010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18565
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182360"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VD791_01310"
     CDS             complement(<1..432)
                     /locus_tag="VD791_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HXV06641.1"
                     /translation="MTEFELIARYFSPATTHTVLAGGDDAALVAVRPGMELVVSTDML
                     VAGRHFCEDAPAYDVGYKSLAVNLSDIAAMGATPRWATLSLALPAADEAWLEAFARGF
                     LELAGTYDVDLIGGDTTRGPLNVCVQIMGEAPAGKALRRSGA"
     gene            complement(425..1291)
                     /gene="panB"
                     /locus_tag="VD791_01315"
     CDS             complement(425..1291)
                     /gene="panB"
                     /locus_tag="VD791_01315"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HXV06642.1"
                     /translation="MAEAVKKLTIFDLQAKVDRGEKIFQVTAVDFPTAQLVDRAGIDC
                     ILIGDSLGMTVLGYASTVPVTMEEMIHHAKAITRAAKRAIVVGDLPLGAYHASSADAI
                     DSAMRMVKEGGADVVKLEGGEDFAPMAAAVVRAGIPVMAHIGLTPQLMSKLGGFRVQG
                     KDAAAGVQLLADALALEKAGCFAIVLEAIPDRVAQLITERLRIPTIGIGAGPHCDGQN
                     LVLHDMVGLFDRFAPKFVKKYADCWALISKALAEFQDEVRSGAFPKAEHSFTMKEEEY
                     QALLQQLPRRGD"
     gene            complement(1320..1787)
                     /gene="nusB"
                     /locus_tag="VD791_01320"
     CDS             complement(1320..1787)
                     /gene="nusB"
                     /locus_tag="VD791_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HXV06643.1"
                     /translation="MPVEKIGVAARERAGRGARRRSRELALQALYQWLVAGHEAGVLC
                     AEAAAGRELQRADKAYFETLVRGVIERAAALDASLQPFLDRPARALSPVEHALLLIGA
                     FELSASPDVPYRVAINEAVELAKAYGGTDGHKYVNGVLDKLAAQLRPGEARKS"
     gene            complement(1798..2277)
                     /gene="ribH"
                     /locus_tag="VD791_01325"
     CDS             complement(1798..2277)
                     /gene="ribH"
                     /locus_tag="VD791_01325"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HXV06644.1"
                     /translation="MARYDDILELEPGLDGAGLRIGIVMSRFNIDVCEGLLAACSAEL
                     RKHGVRPEDMLLVTVPGALEIGLAAQKLAASRRFDAICALGAVIRGETYHFEVVADQS
                     AAALARVQLDSGVPVANGVLTTDDEFQAEARMSHKGADAARAAIEMANLLRRLDGPR"
     gene            complement(2285..3427)
                     /gene="ribBA"
                     /locus_tag="VD791_01330"
     CDS             complement(2285..3427)
                     /gene="ribBA"
                     /locus_tag="VD791_01330"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HXV06645.1"
                     /translation="MSRITPTPEIIAEIQAGRMVVLVDEEDRENEGDLVMAAEFVTPE
                     AINFMARHGRGLICLTLTEARCRQLNLPPMVSNNRSQLGTNFTVSIEAAQGVTTGISA
                     ADRARTVHAAVKRDARPEDLVQPGHIFPLLARNGGVLVRAGHTEAGCDLAELAGLTPA
                     AVICEVLKDDGEMARLPDLIQFAQRHGLKVGTIADLIHYRSRTESLVQRVAERPLETV
                     HGRFRLCAYRDVTAGEVHLALVRGEIVAGKEVLVRVHEPLSVIDALDVASTAHSWNLN
                     DALRTIAAADAGVIVLLHRNETGEALLDRARSQATGAARPKMDLRTYGIGAQILKDLG
                     VTRMKIMALPRKMPSMAGFDLEVTGYLLPERARAGAVVTPLGERKR"
     gene            complement(3508..4098)
                     /locus_tag="VD791_01335"
     CDS             complement(3508..4098)
                     /locus_tag="VD791_01335"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HXV06646.1"
                     /translation="MFTGIVETTGEIASVTPVDQGARVVVRAPAFDLSDVKPGDSVSV
                     DGVCLTAVAVRNQDFSVDVSRETLDRTAGFRPAVQVNLEKALRLSDRLGGHLVSGHID
                     GVGEVTRVESVGGSRLLEVRAPEALGRYIAPKGSIAINGVSLTVNRVSGERFEVNLIP
                     HTLAVTNLQGLRPGGRVNLEIDLIARYLEGLLRARN"
     gene            complement(4138..5235)
                     /gene="ribD"
                     /locus_tag="VD791_01340"
     CDS             complement(4138..5235)
                     /gene="ribD"
                     /locus_tag="VD791_01340"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HXV06647.1"
                     /translation="MFSAADHAHMARALALAQRGLFTAAPNPRVGCVLVRGGEVVGEG
                     WHQRAGEPHAEVHALRAAGERARGATAYVSLEPCSHRGRTPPCAEALVAAGVGRVVAA
                     AEDPNPQVSGRGLARLRAAGIDTACGLLGEQARELNVGFVSRHERGRPWVRVKTAASL
                     DGRTALANGASQWITGPAARRDGHRWRARSCAVMIGIGTLRADDPRLSVRDVETTRQP
                     VRIVIDRYLELPLSARVLEGGNVLVAAAREDAAKVRQIRQAGAEVLVLPDRGGKVDLA
                     QLMRELARREMNEILVESGNRLNGALLRAGLVDELVMYFAPHLLGDLARGMYALGELT
                     ALDQRVDLDLRDLERIGPDLRILARVLPARP"
     gene            complement(5240..5734)
                     /gene="nrdR"
                     /locus_tag="VD791_01345"
     CDS             complement(5240..5734)
                     /gene="nrdR"
                     /locus_tag="VD791_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HXV06648.1"
                     /translation="MKCPFCNSPDTQVVDSRVSEEGDSIRRRRRCLACDKRFTTYETV
                     ELRLPAVVKANGIREEYDEDKLRTGFQRALHKRPVPTEFVDAAIERITQKLLASGERE
                     VDSRRIGEMVMRELYRLDKVAYIRFASVYKDFQDPEDFRDVLKEVARPGGTARKRTRA
                     RREG"
     gene            complement(5749..6993)
                     /gene="glyA"
                     /locus_tag="VD791_01350"
     CDS             complement(5749..6993)
                     /gene="glyA"
                     /locus_tag="VD791_01350"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011356345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HXV06649.1"
                     /translation="MFHKNQSIETFDPDLWRAMAGEAQRQEDHIELIASENYASPRVL
                     QAQGSLLTNKYAEGYPGKRYYGGCEYVDLAEQLAIERARRLFDCDFANVQPHSGSQAN
                     AAVYMAALSPGDTILGMSLAHGGHLTHGSPVNFSGKLYNVVSYGLTEDEVIDYAQVER
                     LAHEHKPKLIVAGASAYSLIIDWKRFREIADAAGASLMVDMAHYAGLVAAGFYPSPLR
                     HAHFVTSTTHKTLRGPRGGLILGHADNEKPINSAIFPGSQGGPLMHVIAAKAVAFKEA
                     MGPEFRAYQEQVLDNARVLAKVLQERGLRIVSGRTESHMFLVDLRAKRITGKEAEAVL
                     GAAHITVNKNAIPNDPEKPFVTSGVRIGSPAITTRGFTELETEQLGNLIADVLEAPQD
                     DGVIRRVAQEVKALCRRLPVYG"
     gene            complement(7107..7397)
                     /locus_tag="VD791_01355"
     CDS             complement(7107..7397)
                     /locus_tag="VD791_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06650.1"
                     /translation="MQYPSESELEACLRTIAVEAGDTSGAPFRISMVRLHGKPPMYSV
                     VILSASKEPMRQRLGKLLARNFSLGVTSIFLTVAEVESLVAHCQMARQDTKA"
     gene            7662..8666
                     /locus_tag="VD791_01360"
     CDS             7662..8666
                     /locus_tag="VD791_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXV06651.1"
                     /translation="MQHFDLAVLIGGFEPLDADQRAAVQAALAAAPRAVVLVGSHGAA
                     RSLRHPWTGEERSAMLRAAFSEAENARLEVLPLRDRLYNEHQWLRNVQAALGPVQPAG
                     ARVVQIALPGQRAPLHPDWSVQQAKPLAAPDFWGVRRGVFGDAGEVAALDGRLPPSTV
                     AFLRRFRAGADFEALAEELRYIERFRDSWRHAPYPPVLVTTDAVVACAGHLLLVHRGH
                     APGKGLWALPGGFLDQDETLLEGCLRELREETGLALAQAALEAALCGSHVFDAPYRSL
                     RGRTITHAYAFALHDGALPSVAGGDDAADARWVPLARFHAMEAQMFEDHYHIARFFLG
                     "
     gene            8680..10500
                     /locus_tag="VD791_01365"
     CDS             8680..10500
                     /locus_tag="VD791_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXV06652.1"
                     /translation="MKNNTPIRYRILPKHPEAHLFETSCSLEDPDPAGQRFALPAWIP
                     GSYLIRDYARHVVGIRAQSARRTLPLAKLDKHTWIAAPCSGPLTVTMEVYARDISGRG
                     AYLDSNQGMFNGASVFLRVPGREHRPCEVDILRPTGARYRNWQVATAMPHRSAPPHGF
                     GAYRAADYDELIDHPVAIGIFTPARFNACGVPHEIAISGRHDTDLDRLCRDLKRLCEW
                     HIRLFGEPPPMQRYLFLITALGEGYGGLEHRASTVLLCSRDDLPRPGQAEVTESYRTF
                     LGLCSHEYFHTWNVKRIKPAAFTPYDLSRENYTRLLWAFEGITSYYDDLALVRAGLIT
                     QQDYFELLGRSITGLLRTPGRHRQTVAEASFDAWIKYYRQDENSPNAQVSYYLKGSLI
                     ALCLDLLIRSRTRGRKSLDHVMQALWARHGRTGAGVEEDGVERLAEEVSGVRLRAFFD
                     RALRSTDELPLRRLLATAGVEMHLRPAESSADKGGKPAPKPSAQRAARTTLGVRTAED
                     ATGVKLTHVLDGGAAQAAGLAAGDVLVAVDGLRVTQRNLDRRLEDLRPGDRVQAHAFR
                     RDELLRCDILLQAPPSDTCFLAAGGDAAARRRRAAWLDPT"
     gene            10532..11509
                     /locus_tag="VD791_01370"
     CDS             10532..11509
                     /locus_tag="VD791_01370"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HXV06653.1"
                     /translation="MTADDRLPVSYRDVAAAAQRLAGQARRTSVLTSRQVDERTGCRV
                     FLKCENFQRVGAFKFRGAFNALSQLAPEQLQRGVLAYSSGNHAQAVALAAQLLGSPAV
                     IVMPQDAPQVKVEATRSYGAEVVLYDKRTAVREQLAAAIAAERGLALIPPYDHPHVVA
                     GQGTAAKELIEDAGPLDYLFVCVGGGGLISGCAIAADALSPGCKVIGVEPAAGDDATR
                     SFKTGTLQMVHNPETIADGARTPSLGRITFPLVLRYVHDMVTVDDAQLLRAMLYVWER
                     MKIVVEPTGALGIAGLFERKVDVPPGSRVGVIVSGGNVDVRDVCRWLGA"
     gene            complement(11520..12548)
                     /locus_tag="VD791_01375"
     CDS             complement(11520..12548)
                     /locus_tag="VD791_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV06654.1"
                     /translation="MRSAGARFVLAAWVALAAACAWAQVPEKKAVTIAVGGKAALYYL
                     PLTLAERLGYFKDAGLDVQILDFPGGAKSLQAMMGGSADVVAGGFDHVVILRARGQKL
                     QAFVLQVATPSLILGVVKARAAGYRSAGDLKGMKVGVTAPGSSTHIFLNHLLAGAGLS
                     ADDVAVIGVGTGPTAVAAVRAGHIDAIVNVEPAIAMLERSGAIVVVTETASEKGSRAQ
                     FGEALPAGSLYTKEEFLKRNPNTAQALVNAMVRALLWLEKASPQEILKTVPAEYALGD
                     PDTYLAALARQRQGYSRDGLIPPAGAAALHRVLRRFDPSVQQARDLDITQAYDNRFAQ
                     QALRKYRK"
     gene            complement(12562..13377)
                     /locus_tag="VD791_01380"
     CDS             complement(12562..13377)
                     /locus_tag="VD791_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV06655.1"
                     /translation="MSAPIPEDTGAPPRVRAMWPWHLLLLAIVVGTWHVLTSTEVLPP
                     FFFGQPLQVGRRILEWLITGKIFPHLGITLLETLLAFVIGTALGLGAGLWLGLSALAA
                     RLLDPYITAVNSMPRVILAPIFAVWFGLGIWSKVLLGVTLVFFIVFFSVYHGVREVSP
                     VVLANARMLGASRRQLLRHVFLPSATAWVFASLHASVGMAFVGAVVGEYLGSARGVGY
                     LILQAEGVFDIDTVFAGVIVLTLCALALDRLVGVLERRLLTWRPVATEAASQS"
     gene            complement(13358..14209)
                     /locus_tag="VD791_01385"
     CDS             complement(13358..14209)
                     /locus_tag="VD791_01385"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV06656.1"
                     /translation="MAPPVQFDERALSGDSPLPALVLDGVTCTFPARGNARDTYTAVR
                     AASLTVQDGEFVSLVGPTGCGKSTLLNVAAGLLAPTQGEVRVFGERLSGLNRVAGYLF
                     QSDALFPWRTALDNVTAGLEFRGVARAEAQQRGMEWLKRVGLAAHASKYPHELSGGMR
                     KRVALAQTWILGPRMLLMDEPFSALDVQTRQLMENELLGLWSQDRRCVLFVTHDLEEA
                     IALSDRVVVLSAGPQSRPIEAFRVDLPRPRDVQEIALHPRFVEVHAAIWRALKQEVLK
                     AHERADS"
     gene            complement(14214..14828)
                     /locus_tag="VD791_01390"
     CDS             complement(14214..14828)
                     /locus_tag="VD791_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HXV06657.1"
                     /translation="MACRRGFAALWLVLLCVGCGRDAPALARLGPDDVVLAFGDSLTF
                     GTGVADDETYPAVLAGLIGRKVVRDGVPGETTAQGLERLEASLETHRPRLLLLCLGGN
                     DLLRRVDAAQIESNLRAMVQLARSRGAEVVLIGVPEPALFGGTAPLYRRLAAELRVLL
                     EEETVTDVLRDEALKSDPIHPNAEGYRRIAQALAALLRDAGAIH"
     gene            complement(14888..16228)
                     /gene="pmbA"
                     /locus_tag="VD791_01395"
     CDS             complement(14888..16228)
                     /gene="pmbA"
                     /locus_tag="VD791_01395"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HXV06658.1"
                     /translation="MPGNAFAHDPDRLRQIASLVLEHARSRGASAAECEVSEGFGQNV
                     TVRRGEVETIEYNRDKGVGVTVYLGKRRGHASTSDFSPQAVRETVDAAHAIAGFTAAD
                     DCAGLADEDLLAREFPDLDLYHPWDLSVEDAIVLARDCEAAALGVDPRIGNSEGGTVS
                     TQQSQFVYANSLGFIGGFPSSRHSIMCSVIGGEGDEMQRDDWYTMARAPSELEPGVAV
                     GRRAGERCVRRLGARRIGTMEAPVLFEAPVAASLLGHFVSAASGGSLYRKASFLVDSL
                     GQRVFAPQVCIRDLPDIPRGLASSPFDDEGVATRRRDVVCDGVLQGYFLGSYSARKLG
                     MRSTGNAGGSHNLVLDSTGEDFEALLRKLGRGLLVTELLGMGVNNVTGDYSRGAAGFW
                     VENGEVAYPVQEITIAGNLKQMFQGIVAAGSDVLVRGSRRCGSILIDRMTVAGE"
     gene            16381..16968
                     /gene="mog"
                     /locus_tag="VD791_01400"
     CDS             16381..16968
                     /gene="mog"
                     /locus_tag="VD791_01400"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HXV06659.1"
                     /translation="MDETLKIGLVSVSDRASRGVYKDEGIPALEQWFRQALTSPWTMI
                     TRLIPDEQPLIQATLEELVDREGCHLVLTTGGTGPAPRDITPEATLAVGHKLMPGFGE
                     QMRQISLHYVPTAILSRQVAVIRGQALIINLPGQPKSIRETLEGVKDGEGKALVHGIF
                     AAVPYCIDLIGGPYIETNAAVVQAFRPKSAIRPKT"
     gene            17047..17718
                     /locus_tag="VD791_01405"
     CDS             17047..17718
                     /locus_tag="VD791_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXV06660.1"
                     /translation="MTAAELLPAIEIETQPRPRSAVIWMHGLGADGSDFVPIVDQLGL
                     PGTAVRFVFPHAPLQPVTINGGYVMRAWYDIGYQDLTLREDEKGVRESQHGVERLIER
                     EIERGIPAGRIVLAGFSQGGAIALQTGLRHSARLAGVLALSSYLPLPDKLEAERSDAN
                     RDVPVFMGHGIEDPIVPLKLAADSCSRLLTMGYPVEWHEYDMPHTVCVEEVAHIGAWL
                     RKVLG"
     gene            complement(17723..>18565)
                     /locus_tag="VD791_01410"
     CDS             complement(17723..>18565)
                     /locus_tag="VD791_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06661.1"
                     /translation="EPAKGCHYYVDLLAAKPPTRMVAKVTPAPAVRYFGPGAAFERVD
                     GLADTIAERNIVPSDMNLGGSYEPGLVLEVARHLARYWASTPPTRREERRRSVARIHV
                     VHNFDDVLDIISGESKSLDFQQNIETWAVQNESDHGFGALIPDSASDWLRVGTLLGIK
                     REDGNAWGVGIVRRLTNDASQQRYVGIQTLAKGGARVKLFPVGAKDGSGGGDDAVLLP
                     SSAADSSGTGELSLLMRMGSFSPRQSFQMRAYEREYLLVPKALLEGGQDFDMAKFRVL
                     QRAA"
BASE COUNT         2784 a         6668 c         6363 g         2750 t
ORIGIN      
        1 cgcgcccgag cggcgcagcg ccttgccggc gggcgcttcc cccatgatct gcacgcacac
       61 gttgagcggc ccgcgcgtgg tgtcgccgcc gatcagatcg acgtcgtagg tcccggccag
      121 ctccagaaag ccgcgcgcga acgcttccag ccacgcctcg tcggccgcgg gcagcgccag
      181 ggacagcgtg gcccagcgcg gcgtggcgcc catcgcggcg atgtcggaca gattcaccgc
      241 cagcgacttg tacccgacgt cgtaggccgg ggcatcctcg cagaagtgcc ggccggcgac
      301 cagcatgtcg gtcgagacca ccagctccat gcctggccgc accgcgacca gcgccgcatc
      361 gtcgccgccg gcgagcaccg tgtgcgtggt ggccggggag aagtagcggg cgatcagctc
      421 gaactcagtc accgcgcctg ggcaactgct gcagcagcgc ctggtactcc tcttccttca
      481 tggtgaagct gtgctcggct ttgggaaagg cgccgctgcg cacctcgtcc tggaactccg
      541 cgagcgcctt ggagatgagc gcccagcagt cggcgtactt cttcacgaac ttgggcgcga
      601 agcggtcgaa caggcccacc atgtcgtgca gcaccaggtt ctgcccgtcg cagtgcggtc
      661 cggcgccgat gccgatggtg gggatacgca ggcgctcggt gatgagctgg gccacgcgat
      721 cggggatcgc ctcgagcacg atcgcgaagc agcccgcctt ctccagggcc agcgcgtccg
      781 ccagcagctg cacacccgcg gccgcgtcct tgccctgcac ccggaagccg ccgagcttgg
      841 acatcagctg cggcgtcagc ccgatgtgcg ccatcaccgg gatgccggcg cgcaccaccg
      901 ccgcggccat cggcgcgaag tcctcgccgc cctcgagttt caccacgtcg gcgccgccct
      961 ccttgaccat gcgcatggcg ctgtcgatcg cgtccgcgga gctggcgtga taggcgccca
     1021 gcggcagatc gccgaccacg atcgcgcgct tcgcggcgcg cgtgatggcc ttggcgtggt
     1081 ggatcatctc ctccatggtg accggcacgg tactggcgta gccgagcacg gtcatgccca
     1141 gcgagtcgcc gatcaggatg cagtcgatgc ccgcgcgatc gaccagctga gcggtgggga
     1201 agtccaccgc cgtcacctgg aagatcttct cgccgcgatc gaccttcgcc tgcaggtcga
     1261 agatcgtcag cttcttcacc gcttcagcca tggtcccctc gcattcgttt gaacagtgct
     1321 caactcttgc gcgcttcccc cggccgcagc tgcgcggcca gcttgtcgag cacaccgttg
     1381 acgtacttgt gcccgtcggt tccgccgtag gccttcgcca gctccaccgc ttcgttgatc
     1441 gccacgcggt agggaacgtc gggcgacgcg ctcagctcga aggcgccgat cagcagcagc
     1501 gcgtgctcca ccgggctgag cgcccgggcc ggccggtcga ggaagggctg gaggctggca
     1561 tcgagagcgg cggcgcgctc gatgaccccg cgcacgaggg tttcgaagta tgccttgtcc
     1621 gcgcgctgca gctcgcggcc cgcggcggct tcggcgcaca ggacaccggc ctcgtggccg
     1681 gcgaccagcc actggtacag ggcctggagc gccagctcgc gggagcgacg gcgcgcgccc
     1741 cggccggcgc gctcgcgcgc ggcgacgccg atcttctcga ccggcattcc cttggcctca
     1801 gcgggggcca tcgagccgcc gcagcaggtt ggccatctcg atcgccgcac gcgccgcgtc
     1861 ggcgcccttg tggctcatgc gcgcctcggc ctggaactcg tcgtcggtgg tcagcacgcc
     1921 attggcgacc ggcacgccgc tgtcgagctg cacacgcgcc agtgccgcgg cggactgatc
     1981 cgcgaccact tcgaagtggt aggtctcgcc gcggatcacc gcgccgagcg cgcagatcgc
     2041 atcgaagcgc cggctggcgg cgagcttctg cgcggcgagc ccgatctcga gcgcaccggg
     2101 cacggtcacc agcagcatgt cttccggccg cacgccgtgc ttgcgcagct ccgccgagca
     2161 ggcggccagc agcccctcgc acacgtcgat gttgaagcgg ctcatgacga tgccgatgcg
     2221 cagccccgcg ccgtcgagcc cgggctccag ctccaggatg tcgtcgtaac gcgccatgca
     2281 agcgctaccg tttgcgctcg cccagaggag tcaccactgc gcctgcgcgc gcgcgctccg
     2341 gcagcagata gccggtgacc tcgaggtcga aacccgccat gctcggcatc ttgcgcggca
     2401 gcgccatgat cttcatgcgc gtcacgccca gatccttgag gatctgcgcc ccgatgccgt
     2461 aggtgcgcag gtccatcttc ggccgcgcag cgcccgtcgc ctgcgaccgc gcccggtcga
     2521 gcagggcctc gccggtctcg ttccggtgca gcaggacgat caccccggca tcggccgctg
     2581 cgatggtgcg caacgcgtcg ttcaggttcc acgagtgcgc ggtgctggcc acatccagcg
     2641 cgtcgatcac cgacagcggc tcgtgcacgc gcaccagcac ttccttgccg gccacgatct
     2701 cgccgcgcac cagggccagg tgcacttcgc cggcggtgac gtcgcggtag gcgcacaggc
     2761 ggaagcggcc gtgcaccgtc tccaggggcc gctccgccac gcgctgcacc agcgactcgg
     2821 tgcggctgcg gtagtggatc aggtccgcga tggtgcccac cttgagcccg tggcgttgcg
     2881 cgaactggat caagtccggc aggcgcgcca tctcgccgtc gtccttgagc acctcgcaga
     2941 tcaccgccgc cggcgtgagc ccggcgagct cggcgaggtc gcagccggcc tcggtgtggc
     3001 cggcgcgcac cagcacgccg ccgttgcgcg ccagcagcgg gaagatgtgg cccggctgca
     3061 ccaggtcctc gggccgcgcg tcgcgcttga ccgccgcatg cacggtgcgc gcgcggtcgg
     3121 cggcggagat gccggtggtc accccctggg ccgcttcgat cgacacggtg aagttggtgc
     3181 ccagttgcga gcggttgttg ctcaccatcg gcggcaggtt gagctgccgg cagcgcgcct
     3241 cggtgagcgt caggcagatc agcccgcgcc cgtgcctggc catgaagttg atcgcctccg
     3301 gcgtcacgaa ctcggcggcc atcaccaggt caccttcgtt ctcgcggtcc tcttcgtcca
     3361 ccaggacgac catgcggccg gcctggatct cggcgatgat ctccggggtg ggggtgatgc
     3421 gggacatgga aggcggtcgc gggctcgttg cgcaagcctg cattctgcca gatcgcgacg
     3481 cggccgcgtg cggctgcagc agggacgcta gttccgcgcc cggagcaggc cctcgagata
     3541 gcgggcgatc agatcgatct cgaggttcac gcgcccgccc ggccgcaacc cctgcaggtt
     3601 ggtcaccgcc agcgtgtgcg ggatcaagtt cacctcgaag cgctcgccgc tcacccggtt
     3661 cacggtgagg ctcacgccgt tgatcgcgat cgagcccttg ggcgcgatgt agcgtcccag
     3721 cgcctcgggc gcacgcactt cgagcagccg gctcccgccg accgattcca cccgcgtcac
     3781 ctcgcccacg ccgtcgatgt ggccgctgac caggtgcccg ccgagccggt cggacaggcg
     3841 cagcgccttc tccagattga cttgcactgc gggccgaaag ccggcggtgc gatcgagcgt
     3901 ttcccgcgac acgtccacgc tgaagtcctg gttgcggacc gcaaccgccg tgaggcagac
     3961 gccgtccacc gagacgctgt cgcccggctt cacgtccgag aggtcgaacg ccggagcgcg
     4021 caccacgacg cgggcgccct gatccaccgg cgtcaccgac gcgatctcac ccgtggtttc
     4081 gacgatgccg gtgaacatga cgccttggac cccggattcc gctgcgctgc atccgggcta
     4141 cggccgggca ggaagaactc gagcgaggat gcgcaggtcc ggtccgatgc gctcgaggtc
     4201 gcgcaggtcc agatccacgc gctggtcgag cgcggtcagc tccccgagcg catacatgcc
     4261 gcgcgccagg tcgcccagca gatgcggcgc gaagtacatc accagctcgt ccaccaggcc
     4321 ggcgcgcagc agcgcgccgt tcaagcggtt gccggactcc accaggatct cgttcatctc
     4381 gcggcgcgcc agctcgcgca ttagctgcgc cagatcgacc ttgcctccgc ggtcgggcag
     4441 caccaggacc tcggcgccgg cctgccggat ctgccgaacc ttggccgcgt cctcgcgcgc
     4501 cgcggccacc agcacgttgc cgccctcgag cacgcgcgcg gacagcggca gctcgagata
     4561 ccgatcgatc acgatgcgca ccggctgccg cgtggtctcg acgtcgcgca cgctgagcct
     4621 cggatcgtcg gccctgagcg tgccgatgcc gatcatgacc gcgcacgacc gcgcgcgcca
     4681 gcgatgaccg tcccggcgcg ccgccgggcc ggtgatccac tggctggcgc cgttggcgag
     4741 cgcggtgcgg ccgtccaggc tggcggcagt cttcacccgc acccagggac gaccgcgctc
     4801 gtggcgcgag acgaaaccga cgttgagctc gcgcgcctgc tcgccgagca ggccgcaggc
     4861 ggtgtcgatg ccggcggcgc gcaggcgcgc cagtccgcgg ccggacacct gcggattggg
     4921 gtcctcggcc gccgccacca cgcggccgac gccggcggcg accagggcct cggcgcaggg
     4981 cggcgtgcgg ccgcgatggc tgcagggctc gagcgagacg taagccgtcg caccgcgcgc
     5041 gcgctcgccg gcggcgcgca gggcgtgcac ctcggcgtgc ggctcgccgg cgcgctggtg
     5101 ccagccttcg cccaccacct cgccgccgcg caccagcacg cagcccaccc gcgggttggg
     5161 cgcggcggtg aacaggccgc gttgcgcgag cgccagcgcg cgggccatgt gggcgtggtc
     5221 ggcggcggag aacatctgct catccttcac gcctggcgcg cgtgcgcttg cgcgccgtgc
     5281 cgcccggcct ggccacctcc ttgagcacgt cgcggaagtc ctcgggatcc tgaaagtcct
     5341 tgtacacgga ggcaaagcgg atgtaggcga ccttgtccag gcggtacagc tcacgcatca
     5401 ccatctcgcc gatgcgccgc gagtcgactt cgcgctcgcc ggaggcgagc agcttctgcg
     5461 tgatgcgctc gatcgccgca tccacgaact cggtgggcac cggtcgcttg tgcagcgcgc
     5521 gctggaagcc ggtgcgcagc ttgtcctcgt cgtactcctc gcggatgccg ttggccttga
     5581 ccacggcggg caggcgcagc tcgacggtct cgtaggtggt gaagcgcttg tcgcaggcga
     5641 ggcagcgccg gcgccggcgg atgctgtcgc cctcctcgct cacgcgcgag tccaccacct
     5701 gggtgtccgg cgaattgcag aacggacact tcatcggggc aggcgcggct acccgtacac
     5761 cggcagccgc cggcacaaag ccttgacttc ctgtgccacg cgccgtatca cgccgtcgtc
     5821 ctgcggcgcc tccagcacgt cggcgatgag gttgccgagc tgctcggtct cgagctcggt
     5881 gaagccgcgg gtggtgatgg cgggcgagcc gatgcggacg ccgctggtga cgaagggctt
     5941 ctccgggtcg ttggggatgg cgttcttgtt caccgtgatg tgagcggcgc cgagcaccgc
     6001 ctcggcttcc ttgccggtga tgcgcttggc gcgcaggtcc accaagaaca tgtgcgattc
     6061 ggtgcggccg gacacgatcc gcagtccgcg ctcctgcagc accttggcca gcacccgcgc
     6121 attgtccagc acctgctcct gataggcgcg aaactccggg cccatggcct ccttgaacgc
     6181 caccgccttg gcggcgatga cgtgcatcag cggcccgccc tgactgccgg ggaagatcgc
     6241 cgagttgatc ggcttttcgt tgtcggcgtg gcccaggatc agccctccgc gcggaccgcg
     6301 cagcgtcttg tgcgtggtgc tggtgacgaa gtgcgcgtgc cgcagcgggc tgggatagaa
     6361 cccggcggcg accagcccgg cgtagtgcgc catgtccacc atcaggctcg cgcccgcggc
     6421 gtcggcgatc tcgcggaagc gcttccagtc gatgatcagg gagtaggcgg aggcgcccgc
     6481 gacgatcagc ttgggcttgt gctcgtgcgc caggcgctcg acctgggcgt agtcgatcac
     6541 ttcgtcctcg gtgaggccgt agctcaccac gttgtagagc ttgccgctga agttgaccgg
     6601 cgaaccgtgc gtgaggtggc cgccgtgcgc cagcgacatg ccgaggatgg tgtcgccggg
     6661 gctgagcgcg gccatgtaga cggcggcgtt ggcctggctg ccggaatgcg gctgcacgtt
     6721 ggcgaagtcg cagtcgaaca gccgtctggc gcgctcgatg gccagctgct cggcgaggtc
     6781 cacgtactcg cagccgccgt agtaacgctt gccggggtag ccttcggcgt acttgttggt
     6841 gagcagcgac ccctgcgcct gcagcacccg cgggctggcg tagttctcgg aagcgatcag
     6901 ctcgatgtgg tcttcctggc gctgggcctc gcccgccatg gcccgccaca ggtccgggtc
     6961 gaaggtctca atgctttgat ttttgtgaaa catggggcgg ctcgggaaag ccccgattgt
     7021 agcgttaaga ctgcgagccg cccaccgcga cagggtacag ggggttggtt ttcttgagtt
     7081 cgcgccttaa gggcgcacca ggcgcattaa gccttggtgt cctggcgggc catctgacaa
     7141 tgggccacca gcgactcgac ttcggccacg gtgaggaaga tcgaggtcac gccgaggctg
     7201 aagttacgcg ccaggagctt tcccagccgc tggcgcatcg gttccttgga ggcggacagg
     7261 atcaccaccg agtacatcgg cggcttgccg tgcaggcgga ccatgctgat ccggaacgga
     7321 gcgccgctgg tgtccccggc ttcgaccgcg atggtgcgca ggcaagcctc gagctcggat
     7381 tcggaggggt actgcatgag agcattctcg accgaagcga ccggagggcg ttcgcaacgc
     7441 ccccgatggt ccatggccta gattctaatc aaatccgccc ttactaagcg agcggcatcg
     7501 gatcgcccac cgcagaccgg gccttgcggt cgcacccgac gcttgcagtc gcaccgggga
     7561 gcgctcgcgt agccggtcgg agacgcgcga ccgcgggggg ggggcccggc ggctatactc
     7621 gcggcatctc ccgtccgccc gccaagcgct acatcccatc catgcagcat ttcgacctcg
     7681 cggtcctgat cggcggcttc gagccgctcg acgccgacca gcgagcggcg gtccaggccg
     7741 ccctggcggc ggcccctcgt gcggtcgtgc tggtgggctc ccatggcgct gcccgcagcc
     7801 tgcgccatcc gtggaccggc gaggagcgca gcgcgatgtt gcgcgccgcg ttcagcgagg
     7861 ccgagaacgc gcgcctcgag gtgctgccgc tgcgcgaccg cctgtacaac gaacaccagt
     7921 ggctgcgcaa cgtgcaggcg gcactgggtc ccgtgcagcc tgctggcgcc cgcgtcgtgc
     7981 agatcgcgct tcccggccag cgcgcgccgc tgcaccccga ttggtccgtg cagcaggcaa
     8041 aacctctggc ggcccccgat ttctggggcg tgcggcgcgg agtgttcggc gacgcggggg
     8101 aggtcgcggc cctggacggc cgcttgccgc cctcgacggt cgcctttctg cgccgctttc
     8161 gcgccggcgc cgatttcgaa gcgcttgccg aggagctgcg ctacatcgag cgcttccgcg
     8221 acagctggcg ccatgccccc tacccgcccg tgctggtcac cacggacgcg gtggtggcct
     8281 gcgccggaca cctgttgctg gtgcaccgcg gccacgcacc gggcaagggc ctgtgggcgc
     8341 tgccgggcgg cttcctcgac caggacgaga cgctgctcga gggttgcctg cgcgagctgc
     8401 gggaagagac cggtctggcg cttgcgcaag ccgcgcttga ggccgccctg tgcggcagtc
     8461 atgtgttcga tgcgccgtat cgctcgctgc gcggccgcac catcacccac gcctacgctt
     8521 tcgcgctcca cgacggcgcg ctgccgagcg tcgccggcgg cgacgacgcc gcggacgcac
     8581 gctgggtgcc gctggcgcgc ttccacgcca tggaggcgca gatgttcgaa gatcattatc
     8641 acatcgccag gttcttcctc ggctaggcga gttgcataca tgaagaacaa cacaccgatt
     8701 cgctaccgga tccttcccaa acatcccgag gcccatctgt tcgaaacgtc ctgcagcctc
     8761 gaggaccccg accccgccgg acagcgcttc gcgctgccgg cctggatccc cggcagctat
     8821 ctgatccgcg actacgcccg ccacgtcgtc gggatacggg cgcagagcgc acgccgcacg
     8881 ctgccgctcg ccaagctgga caagcacacc tggatcgccg cgccctgcag cggcccgctc
     8941 accgtgacca tggaggtgta cgcccgggac atctcgggtc gcggcgccta tctggattcg
     9001 aaccaaggca tgttcaacgg ggcgtcggtg ttcctgcgcg tgcccggccg ggagcaccgg
     9061 ccttgcgagg tggacatcct ccggcccacc ggcgcgcgct atcgaaactg gcaggtggcg
     9121 accgccatgc cgcacaggag cgcgccgccg cacggcttcg gcgcctaccg ggcggccgat
     9181 tacgacgagc tgatcgacca cccggtggcc atcggcatct tcaccccggc ccggttcaac
     9241 gcctgcggcg tgcctcacga aatcgccatc agcggccgcc acgacaccga tctcgaccgc
     9301 ctgtgccgcg atttgaagcg cctgtgcgaa tggcacatcc gcctgttcgg agagccgccg
     9361 ccgatgcagc gctatttgtt tctgatcacc gcgctcggcg agggctacgg cgggctcgag
     9421 caccgcgcct ccaccgtgtt gctgtgctcg cgcgacgacc tgcccaggcc gggacaagcc
     9481 gaggtcacgg agagctaccg gacctttctg ggcttgtgca gccacgagta cttccacacc
     9541 tggaacgtca agcgcatcaa gcccgccgcc ttcacgccct acgacctgag tcgggagaac
     9601 tacacgcggc tgctgtgggc cttcgaaggc atcaccagct actacgacga tctggcgctg
     9661 gtgcgcgccg gcctgatcac gcaacaggat tacttcgagc tgctgggcag aagcatcacc
     9721 ggcttgctgc gcaccccggg gcgccacaga cagaccgtgg ccgaggcgag cttcgacgcc
     9781 tggatcaagt actaccgtca ggacgagaac agcccgaacg cgcaagtcag ctactacctg
     9841 aaaggcagct tgatcgccct gtgcctggac ctgctgatcc gcagccgcac gcgcgggcgc
     9901 aagtccctcg accacgtgat gcaggcgctg tgggcgcgac acggccgcac cggcgcgggc
     9961 gtcgaggagg acggcgtgga gcgcctcgcc gaggaggtga gcggcgtgcg gctacgggca
    10021 ttcttcgatc gcgcgctgcg ctccaccgac gagctgccgc tgaggcggct gctcgccacg
    10081 gcgggcgtcg agatgcacct gcgccccgcc gaatccagcg ccgacaaggg cggcaaaccc
    10141 gcccccaagc cgagcgccca gcgcgccgcc cgcaccacgc tgggcgtgcg caccgccgag
    10201 gatgcgacgg gcgtcaagct cacacacgtg ctcgatggcg gcgccgcgca ggccgcggga
    10261 ctggccgcgg gcgacgtgct ggtggccgtc gacggcttgc gcgtcaccca gcgcaacctg
    10321 gaccggcgcc tggaagacct gcgaccgggc gatcgcgtcc aggcgcacgc attccgccgc
    10381 gacgagctgc tccgttgcga tatcctgctg caggcgcccc cgtccgatac ctgcttcctg
    10441 gcggcgggcg gcgacgccgc cgcacggcgg cggcgcgcgg catggctgga cccgacgtga
    10501 agcaatccac ccgctgcccg gcgttcgccc gatgaccgcg gacgaccgtc tccccgtctc
    10561 ctatcgggac gtggccgccg ccgcgcaacg gcttgccggc caggcccgcc gcacgtcggt
    10621 cctcacctcg cgccaggtgg acgagcgcac cggctgccgc gtgttcctca agtgcgagaa
    10681 cttccagcgc gtcggggcgt tcaagttccg cggggcgttc aatgcgctgt cgcagctcgc
    10741 gccggagcag ctgcagcgcg gcgtgctcgc ctattcgtcg ggcaaccacg cgcaggcggt
    10801 ggcgctggcc gcgcagctgc tcggcagccc ggcggtcatc gtcatgccgc aggacgcgcc
    10861 gcaggtcaag gtggaggcga cccgcagcta cggcgccgaa gtggtcctgt acgacaagcg
    10921 caccgccgtg cgcgagcagc tggccgcagc gatcgccgct gagcgcgggc tggctctgat
    10981 cccgccctac gaccacccgc acgtggtggc gggccagggg acggcggcaa aggagctgat
    11041 cgaggacgcc ggtccgctgg attacctgtt cgtgtgcgtc ggcggcggcg gcctgatctc
    11101 gggctgcgcg atcgcggccg atgcgctgtc gccgggctgc aaggtgatcg gcgtcgagcc
    11161 cgcggccggc gacgacgcca cgcgctcgtt caagaccggg acgctgcaga tggtgcacaa
    11221 ccccgagacc atcgccgacg gcgcgcgcac gccctcgctg ggccggatca ccttcccgct
    11281 ggtgctgcgc tacgtgcacg acatggtcac ggtcgacgac gcgcagctcc tgcgggcaat
    11341 gctctacgtc tgggagcgca tgaagatcgt ggtggagccc accggcgcgc tgggcatcgc
    11401 cggcctgttc gagcgcaagg tggacgtgcc ccccgggtcg cgcgtgggtg tgatcgtttc
    11461 cggcgggaat gtggacgtac gcgacgtgtg ccgctggctg ggcgcctgac cgccccgcgc
    11521 tacttccggt acttgcgcaa cgcctgctgt gcgaaccggt tgtcgtaggc ctgcgtaatg
    11581 tccaggtcgc gggcctgctg caccgaagga tcgaaacgcc gcagcacgcg atgcagggcg
    11641 gcggctcccg ccggcgggat cagcccgtcg cgcgagtacc cctgccgctg acgcgcgagc
    11701 gccgccagat aggtatcggg gtcgcccagc gcgtactcgg cgggcaccgt tttcaggatc
    11761 tcctgcgggc tcgccttctc cagccacagc agcgcacgca ccatggcgtt caccagcgct
    11821 tgcgcggtgt tcgggttgcg cttgaggaac tcctccttgg tgtacaggga gccggcgggc
    11881 aacgcctcgc cgaactgcgc gcggctgccc ttctcgctgg cggtctcggt caccacgacg
    11941 atggcgccgc tgcgttcgag catggcgatc gcgggctcga cgttgacgat cgcgtcgata
    12001 tgtccggccc gcacggcggc tactgcggtc gggccggtgc ccacgccgat cacggcgacg
    12061 tcgtccgcgg aaagcccggc gccggcgagc agatgattga ggaagatgtg cgtgctcgat
    12121 cccggcgcgg tgacgccgac cttcatgccc ttcagatccc cggcggagcg atagcccgcc
    12181 gcgcgcgcct tgaccacgcc gaggatcagc gacggcgtgg ccacctgcag cacgaacgcc
    12241 tgcagcttct gcccgcgcgc gcgcaggatc accacgtgat cgaagccgcc ggcgacaacg
    12301 tccgcgctgc cgcccatcat cgcctgcagc gacttggcgc caccgggaaa atccaggatc
    12361 tgcacgtcga ggcccgcatc cttgaagtag ccgaggcgtt cggccagagt caacggcaga
    12421 tagtagagcg cggccttgcc cccgaccgcg atggtgaccg ccttcttctc agggacctgc
    12481 gcccaggcgc aggccgcggc cagcgccacc cacgccgcca ggacaaaacg cgcccctgcc
    12541 gatctcatcg tcgattccgc ctcaggactg cgaagccgct tccgtggcca ccggccgcca
    12601 ggtcagcagg cgccgctcca gcacgccgac cagccggtcg agggccagcg cgcacagcgt
    12661 cagcacgatc acaccggcga acacggtgtc gatgtcgaac acgccctcgg cctgcaggat
    12721 cagatagccc accccgcggg cggagccgag atactcgccc accaccgcac cgacgaaggc
    12781 catgcccacg gaggcgtgca ggctggcgaa cacccaggcg gtggcgctgg gcaggaacac
    12841 gtggcgcagc agctgtcggc ggctggcccc cagcatgcgc gcgttggcga gcaccaccgg
    12901 gctgacttcg cgcacgccat gatacacgct gaagaacacg atgaagaaca ccagcgtcac
    12961 gccgagcagc accttggacc agatgcccag cccgaaccac accgcgaaga tcggcgcgag
    13021 gatgacgcgc ggcatggaat tcaccgcggt gatgtaggga tcgagcaggc gcgcggcgag
    13081 cgcgctcagc cccagccaca ggcccgcgcc cagcccgagc gctgtgccga tgacgaaagc
    13141 aagcagcgtc tccagcaagg tgatgccgag gtgcgggaag atcttgccgg tgatcaacca
    13201 ctccaggatg cgccggccca cctgcagcgg ctggccgaag aagaacggcg gcagcacctc
    13261 ggtgctggtg agcacgtgcc aggtgcccac tacgatcgcc agcagcagaa ggtgccacgg
    13321 ccacatggcg cgcacgcgcg gcggcgcgcc ggtgtcctca ggaatcggcg cgctcatggg
    13381 ccttcagcac ctcctgcttg agagcgcgcc agatggcggc atgcacctcg acgaaacgcg
    13441 ggtgcagcgc gatctcctgc acgtcgcgcg ggcgcggcag atcgacgcgg aacgcctcga
    13501 tcgggcgcga ctgcggtccg gccgagagca ccaccacgcg atcggacagg gcgatggcct
    13561 cctccaggtc gtgggtcacg aacagcacgc agcgacggtc ctgcgaccac agcccgagca
    13621 gctcgttctc catcagctgc ctggtctgca cgtcgagcgc gctgaacggc tcgtccatca
    13681 gcagcatgcg cggcccgagg atccaggtct gcgccagcgc cacgcgcttg cgcattccgc
    13741 ccgacagctc gtgcggatac ttggacgcat gcgcggccag gccgacgcgc ttgagccact
    13801 ccatgccgcg ctgctgcgcc tcggcgcgcg ccacgccgcg gaactccagc cccgcggtga
    13861 cgttgtcgag cgccgtgcgc cagggaaaca gggcgtcgga ctgaaacagg taaccggcga
    13921 cccggttcag gcccgacaag cgctccccga acacccggac ctcgccctgc gtgggcgcga
    13981 gcaacccggc cgcgacgttg agcagcgtgg acttgccgca gccggtgggg ccgaccagcg
    14041 acacgaactc gccgtcctgc accgtgagcg aagccgcacg caccgccgtg tacgtatcgc
    14101 gagcgttacc gcgtgcggga aaggtgcagg tgacgccgtc cagcacgagg gcgggcagcg
    14161 ggctatcgcc ggacagcgcc ctctcgtcga actgaacagg cggcgccatc gcctcagtgg
    14221 atcgcgcccg catcgcgcaa cagcgcggcg agggcctgcg cgatgcgacg gtagccctcg
    14281 gcgttgggat ggatcgggtc ggacttgaga gcctcgtcgc gcagcacgtc ggtgaccgtc
    14341 tcttcctcca gcagcacgcg cagctcggcc gcgaggcgcc ggtacaacgg cgccgtcccg
    14401 ccgaacagag ctggttccgg gacgccgatc agcaccacct cggcgccgcg gctgcgcgcg
    14461 agctgcacca tggcgcgcag attcgactcg atctgcgccg cgtccacgcg gcgcagcaga
    14521 tcgttgccgc ccagacacaa cagcagcaac cgcgggcgat gcgtctcgag cgacgcctcc
    14581 agccgctcca gtccctgcgc ggtggtttcc ccggggaccc cgtcgcgcac caccttgcgc
    14641 ccgatcaggc cggccagcac cgcggggtag gtctcgtcgt cggcgacccc ggtgccgaag
    14701 gtgaggctgt cgccgaatgc gagcacgaca tcgtccgggc ccaaccgggc cagcgcgggc
    14761 gcatccctgc cgcagcccac gcacagcagg accagccaca gggcggcgaa tccgcgccga
    14821 cacgccacgg gcgcaaacgc atgtctcact gcgcgccgct cacccccgcg ttggccgcgt
    14881 gccggcctca ctcccccgcg accgtcatgc ggtcgatcag gatcgagccg cagcggcgcg
    14941 agccgcgcac caggacgtcg ctgcccgcgg ccacgatccc ctggaacatc tgcttcaggt
    15001 ttccggcgat cgtgatctcc tgcaccggat aggcgacctc cccgttctcc acccagaacc
    15061 cggcggcgcc gcgcgagtag tcgccggtga cgttgttcac gcccatcccc agcagctcgg
    15121 taaccagcag cccgcgcccg agcttgcgca gcagcgcctc aaagtcctcg ccggtgctgt
    15181 cgagcaccag gttgtggctg ccgccggcgt tgccggtgga gcgcatgccg agcttgcgcg
    15241 ccgagtagct gccgaggaag tagccctgca gcacgccgtc gcacaccacg tcgcggcggc
    15301 gggtggccac gccttcgtcg tcgaacgggc tgctcgccag gccgcgcggg atgtcgggca
    15361 ggtcgcggat gcacacctgc ggcgcgaaca cgcgctgccc caggctgtcc accaggaagg
    15421 aagccttgcg atacaggctg ccgccgctcg cggccgagac gaaatggccg agcaggctgg
    15481 cggcaaccgg cgcttcgaac agcaccggcg cttccatggt gccgatccgg cgcgccccga
    15541 gccggcgcac gcagcgttcg cccgcgcgcc gcccgaccgc cacgccgggc tccagctcgg
    15601 aaggggcgcg cgccatggtg taccagtcgt cgcgctgcat ctcgtcgcct tcgcccccga
    15661 tcaccgagca catgatgctg tggcgcgagc tgggaaaccc gccgatgaag ccgaggctgt
    15721 tggcgtagac gaactgcgac tgctgcgtgg agacggtgcc gccctcggag ttgccgatgc
    15781 gcgggtcgac gccgagcgcc gcggcttcgc agtcgcgcgc caggacgatc gcatcctcga
    15841 cgctcagatc ccagggatgg tacagatcca gatccggaaa ctcgcgcgcc agcagatcct
    15901 cgtcggcaag cccggcgcaa tcgtcggcgg cggtgaagcc ggcgatggca tgggccgcgt
    15961 ccaccgtttc gcgcaccgcc tgcggcgaga agtccgaagt gctggcgtgg ccgcgccgct
    16021 tgccgagata gacggtgacg cccacgccct tgtcgcggtt gtactcgatg gtttcgacct
    16081 cgccgcggcg caccgtgacg ttctgcccga aaccctcgct gacttcgcat tccgcggcgc
    16141 tcgcgccgcg gctgcgcgcg tgctcgagca ccagggacgc gatctggcgg agtcgatccg
    16201 ggtcgtgggc gaaggcgttg ccgggcacgg tgcagtcgcg cgagaaatgg gatgagcggc
    16261 cgctcacgcc gcgcacattg ttatgatacc agctggctcc ggtggagccg cgcgtgggga
    16321 aacggcggct ggacaacctg caaccgcgcg gaccgtgcgc gtgaggcatg cgtgactgcg
    16381 atggacgaga ctctcaagat cggtttggtg tcggtgagcg accgcgcctc ccgcggcgtg
    16441 tacaaggacg agggcatccc ggccctggag cagtggtttc gccaggcgct gacctcgccc
    16501 tggaccatga tcacgcggct gatcccggac gagcagccgc tgatccaggc cacgctggag
    16561 gagctggtgg atcgcgaggg ctgccacctc gtgctgacca cgggcggcac cggaccggcg
    16621 ccgcgcgaca tcacaccgga ggccaccctg gcggtgggcc acaagctgat gccgggcttc
    16681 ggcgagcaga tgcgccagat cagcctgcac tacgtgccca ccgcgatcct ctcgcgccaa
    16741 gtcgcggtga tccgcggaca ggccctgatc atcaatctcc cgggacagcc gaagtcgatc
    16801 cgggagacgc tggagggggt caaggacggc gaaggcaagg cgctggtgca cggcatcttc
    16861 gcggcggtgc cctattgcat cgacttgatc ggcggcccct acatcgagac caatgcggcc
    16921 gtggtgcaag ctttccgtcc caagtccgcg atccggccca agacctgagc agactcgcgg
    16981 cgtgaactgt gcaccgttgg ctcgcctatt gcttggggcc ccggattgtc ccgctttccc
    17041 atcacgatga cagctgccga actgctcccc gccatcgaga tcgagacaca gccgcggccg
    17101 cgcagcgctg tgatctggat gcacggtctg ggtgcggacg gcagcgactt cgtgccgatc
    17161 gtcgaccagc tcggcctgcc cggaaccgcc gtgcgcttcg tgttcccgca cgcgccgctg
    17221 cagccggtca ccatcaacgg cggctacgtg atgcgcgcct ggtacgacat cggctaccag
    17281 gacctgacgt tgcgcgagga cgagaagggg gtgcgcgaat cgcagcacgg cgtcgagcgc
    17341 ctgatcgagc gcgagatcga gcgcggcatt ccggccggcc gtatcgtgct cgccggcttc
    17401 tcccagggcg gcgcgatcgc gctgcagacc ggcctgcgcc acagtgccag gctcgccggc
    17461 gtcctggcgc tgtcgagcta tctgcccctg cccgacaagc tcgaagccga gcgcagcgac
    17521 gcgaaccggg acgtgccggt gttcatggga cacggcatcg aggacccgat cgttcccttg
    17581 aagctcgcgg ccgactcctg cagccgcctg ctcaccatgg gctaccccgt ggagtggcac
    17641 gagtacgaca tgccgcacac cgtgtgcgtc gaggaagtgg cgcacatcgg ggcgtggctg
    17701 cgcaaggtgc tgggctaacc gctcacgccg cgcgctgcag cacccggaac ttggccatgt
    17761 cgaagtcctg gccgccctcg agcagggcct tgggcaccag cagatattcg cgctcgtagg
    17821 cgcgcatctg gaagctctgg cgcggcgaga agctgcccat gcgcatcagc aggctgagct
    17881 cgccggtgcc gctgctgtcc gcggcgctgc tgggcagcag caccgcgtcg tcacctccgc
    17941 cggatccgtc cttcgctccc accgggaaca gcttcacgcg cgcgccgccc ttggccagcg
    18001 tctggatgcc gacgtagcgt tgctgggacg cgtcgttggt gagccggcgc acgatgccca
    18061 cgccccaggc gttgccgtcc tcgcgcttga tgccgagcag cgtgccgacc cgcagccagt
    18121 cgctggccga gtcggggatc agcgcgccga agccgtggtc gctctcgttc tgcaccgccc
    18181 aggtctcgat gttctgctgg aaatcgaggc tcttggactc gccggagatg atgtcgagca
    18241 catcgtcgaa attgtgcacg acgtggatgc gggcgacgct gcgccgtcgc tcctcgcgcc
    18301 gcgtcggcgg cgtcgaggcc cagtagcgcg ccaggtggcg ggcgacctcg agcaccaggc
    18361 cgggctcgta gctgccgccg aggttcatgt cggaaggcac gatgttgcgc tcggcgatcg
    18421 tgtccgccag gccgtcgaca cgctcgaaag cggcgccggg gccgaagtag cgcaccgccg
    18481 gcgccggcgt caccttcgcc accatgcggg tgggcggctt ggcggccaac agatccacgt
    18541 aatagtggca acccttggcc ggctc
//
LOCUS       DASPGE010000043         4966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_597293, whole genome shotgun sequence.
ACCESSION   DASPGE010000043 DASPGE010000000
VERSION     DASPGE010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597293"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VD791_01415"
     CDS             <1..964
                     /locus_tag="VD791_01415"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HXV06662.1"
                     /translation="GDAADSARDGAGAPNYLWHAVKGFFDNGGGRVYVTRLQGDASEV
                     DERALAEALRGLEANEEVSLLCAPGVRLAQGHAQLCNLLLRHCERTSGRFALLDTPPG
                     AAPDAARALRGHLQSDRGALYYPWIAVQAAAPIGAADAAGIVVPPSGHLAGLYADSDD
                     QPGIHKAPANLPIAGALGLERPVGDADYARLAPLGVNCLRSPPGRRGAWVWGARTLGT
                     DSGWNYVAVCRYLLYLRKALEHGLRWVHAQPGSGRLWEDVTALVSEFLYREWRMGALM
                     GGTPAEAYAVQCQPGAGRATLAVGVALVQPADFVRFSTELAARA"
     gene            1038..1496
                     /locus_tag="VD791_01420"
     CDS             1038..1496
                     /locus_tag="VD791_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV06663.1"
                     /translation="MPITELNHYFVRANDLEKTKDFYVGVLGFEVMERPNFPFPGYWL
                     GVNGKIQVHMGPHGISNAELYYLGTPSGAATDQTGVIDHIAFLATDPTAFRQRLAQMQ
                     VDYRPRYLPEAQLYQLFIKDPNGLTIELNFFGITGNPDWGGEDYSRMPKA"
     gene            1703..2365
                     /locus_tag="VD791_01425"
     CDS             1703..2365
                     /locus_tag="VD791_01425"
                     /inference="COORDINATES: protein motif:HMM:NF017517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06664.1"
                     /translation="MSGDGSAYRLAVVGAGRVRGQIAVAACPGRVGKMPLPSHSRWCL
                     QRDVTTLRHWGAEALVTLLEKSELLSMRLGELPALLAAQGVAWHHVPLPEDSIPDMRF
                     EAVWQPVSRYLRGVLWRGGRVALHCADGRSRAPLVTAKLLIELGCPLRDALNRVRSAR
                     PGVLLRPEEVEFLLTQGAGDAYVVPGVVPGPAPLPVSAADDGGQFALIHNPNQLELLR
                     AS"
     gene            2526..3086
                     /locus_tag="VD791_01430"
     CDS             2526..3086
                     /locus_tag="VD791_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HXV06665.1"
                     /translation="MTRSSVQSAIVHRVALTCHPATPCTAVQRIEVEVREAADAGLEL
                     RYLLDGAIERVLIPAAGAPRRADRLWQHTCFEAFLAGEAQGAYFEYNFAPSEEWAVYR
                     FSAYREGMAPVEPGRAPRIRSEATPRRLQLDAEIDAPLAARRIALSAVVEDVDRRLSY
                     WALAHPLAKPDFHHPDAFALRLEHAT"
     gene            3083..4288
                     /locus_tag="VD791_01435"
     CDS             3083..4288
                     /locus_tag="VD791_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HXV06666.1"
                     /translation="MRFGLDRLLEEPALRKPLAGRRIALLAHPASVTRDLRFSLDALA
                     ALGDLHIVAAFGPQHGLRGDKQDNMIESPDYRDPEHGIPVFSLYGAVRRPTAAMLDCF
                     DVLLVDLQDVGCRIYTFITTLRYVLEDAARAHKAVWVLDRPNPAGRPVEGLRLRPGWE
                     SFVGAGALPMRHGLTMGELARWFVHTLALDVDCRVVEMQGWEPARAPGYGWPLGERAW
                     VNPSPNAPNLWMVRCYAGTVMLEGTTLSEGRGTTRPLELFGAPGLDVGELLARMRALA
                     PHWLQGCRLRPCWFEPTFHKHAGKLCAGVQIHVEDGAYEHEAFRPWRLQALAFKALKQ
                     LRPDYALWRDFPYEYERNRLAIDLINGSALLREWVDDAAATPADLDALAQPDEAAWRE
                     ERDAILLYR"
     gene            4321..4635
                     /locus_tag="VD791_01440"
     CDS             4321..4635
                     /locus_tag="VD791_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06667.1"
                     /translation="MLIDRWLTRWSRNYAFLRRALDDIGRQLARRPYDAMLQPDEELS
                     FSQMVQDVRVDFEVEVFRIDLSDRTMWVRVTARSDLATPLRLKPSLVFRKLPDGRAYI
                     FL"
     gene            complement(4705..>4966)
                     /locus_tag="VD791_01445"
     CDS             complement(4705..>4966)
                     /locus_tag="VD791_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HXV06668.1"
                     /translation="APGVDPVTLVPYAPQMVDQVAYESMRALRKPFALVHEYSFYALL
                     VLVVLHVAAVVVTEVREGGSLVSAMFTGRKIVPGHPVDEERD"
BASE COUNT          683 a         1781 c         1756 g          746 t
ORIGIN      
        1 cggcgacgcc gcggacagcg cgcgcgacgg cgccggcgcg ccgaactacc tgtggcacgc
       61 cgtcaagggc ttcttcgaca acggcggcgg gcgcgtgtac gtgacgcggc tccagggcga
      121 tgcctcggag gtggacgagc gcgcgctcgc cgaggcgttg cgcgggctcg aggccaacga
      181 ggaggtgtcg ttgctgtgcg cgcccggcgt ccggctggca cagggccacg cgcagctgtg
      241 caacctgctg ctcaggcact gcgaacgcac cagcgggcgc ttcgcgctgc tcgacacgcc
      301 gccgggcgcc gcacccgacg cggcacgcgc gctgcgcggg catctgcaat cggatcgcgg
      361 cgcgctctac tacccctgga tcgccgtgca ggccgccgcg cccatcggcg cggccgacgc
      421 cgccgggatc gtggtgccgc cctcgggcca tctcgccggc ctgtacgccg acagcgacga
      481 tcaacccggc atccacaagg cgccggccaa tctgccgatc gccggcgcgc tgggcctgga
      541 acggcccgtc ggcgatgccg actacgcccg gctcgcaccg ctcggcgtca actgcctgcg
      601 ctcgccgccg ggccggcgcg gagcatgggt gtggggcgca cgcacgctgg gcaccgattc
      661 gggctggaac tacgtcgccg tgtgccgcta cctgctgtac ctgcgcaagg cactcgagca
      721 cggcctgcgc tgggtgcatg cgcaacccgg cagcggccgg ctgtgggagg acgtcaccgc
      781 cctggtttcg gagtttcttt accgggaatg gcgcatgggc gcgctgatgg gcggcacgcc
      841 cgcggaagcc tacgccgtcc aatgccaacc gggcgcgggc cgcgccacgc tggcagtcgg
      901 cgtggcgctg gtccagccgg cggatttcgt gcgtttcagt accgagctcg cggcccgcgc
      961 ctgacgaacg ggcgcatccg gtttatgctc tcgccgcagg cgatcggcgg ccgcggccgc
     1021 cgcaccggga gacagacatg ccgatcaccg agctcaacca ctacttcgtg cgcgccaacg
     1081 acctggagaa gaccaaggac ttctacgtgg gcgtcctcgg attcgaggtg atggagcgcc
     1141 cgaactttcc gttcccgggc tactggctcg gggtgaacgg caagatccag gtgcacatgg
     1201 ggccgcacgg catctccaac gccgagctgt actacctggg caccccgtcc ggcgccgcca
     1261 ccgatcagac cggggtgatc gaccacatcg ccttcctcgc caccgacccg acggcgtttc
     1321 ggcagcggct ggcgcagatg caggtcgact accggccgcg ttatctgccc gaggcgcagc
     1381 tctaccagct gttcatcaag gaccccaacg ggctcaccat cgagctcaat ttcttcggca
     1441 tcaccggcaa cccggactgg ggcggcgaag actactccag gatgccgaag gcatagcggg
     1501 tccgattgcc ccgaggacct cgggcaagcc ggaaaccgct tccgccgcgt cgcgcgcggt
     1561 gcgccaccgg ccgccgcgtt cgtcgccgtt cgccgacagt gttgccgagc tgcaggcggg
     1621 cgctgaaata tttctggcgc tgtgctgcgc tgatttgcta cctttgtatc tccgccgact
     1681 gaaacgccga ggaggagacc agatgagcgg agacggttcg gcctatcgac tcgccgtggt
     1741 cggagccggg cgcgtgcgcg gccagatcgc agtggccgcc tgccccggac gcgtcggcaa
     1801 gatgccgttg ccctcccaca gccgctggtg cctgcagcgc gacgtcacga cgctgcggca
     1861 ctggggcgcc gaggccctgg tcaccctgct ggagaaatcg gagctgctgt ccatgcggct
     1921 gggcgagctg cccgccctgc tcgccgcgca aggtgtcgcc tggcatcacg ttcccctgcc
     1981 tgaggacagc atccccgaca tgcgtttcga agcggtgtgg caaccggtga gccggtacct
     2041 gcgcggcgtg ttgtggcgcg gggggcgtgt cgccctgcac tgcgcggacg gcaggtcgcg
     2101 cgcaccgctg gtgacggcca agctgctgat cgagctcggt tgcccgctgc gcgacgcgct
     2161 caaccgcgtg cgcagcgcgc gtcccggggt tctgctgcgt cccgaggagg tggagttcct
     2221 gttgacgcag ggcgcgggcg acgcctatgt ggtcccgggc gtggtcccgg gtcccgcccc
     2281 gctgccggtg tcggcggcgg acgatggcgg gcagtttgcg ctgatccaca accccaacca
     2341 gctcgagctg ctgcgggcat cctgagacgg ttgcggcctc ggggcagcga acgcgcgctg
     2401 cgcgaacggc cggcggcggc ccgttgactg cgccggggtg agcgtcaccg cccagagcgc
     2461 gggggtcggc tggcaccgcc ggggccgcaa gatcggcctg gcgctgccgt tacaattcct
     2521 gccctgtgac ccgatcctcc gttcagagcg cgatcgtcca ccgcgtcgcg ctcacctgcc
     2581 acccggccac gccctgcacg gccgtgcagc gcatcgaggt ggaggtgcgc gaagcggccg
     2641 atgccgggct cgagctccgc tacctgctgg acggcgccat cgagcgcgtg ctgatccccg
     2701 ccgcaggcgc gccccggcgc gcggaccggc tctggcagca cacctgcttc gaggcgtttc
     2761 tcgccgggga ggcgcagggc gcgtacttcg agtacaactt cgcaccctcg gaagaatggg
     2821 ccgtctaccg cttcagcgct tatcgcgagg ggatggcgcc ggtcgagccg gggcgtgcgc
     2881 cgcgcatccg gtccgaggcc acgccccgcc gcttgcagct cgatgccgag atcgacgcgc
     2941 cgctggctgc gcggcggatc gcgctgtccg ccgtggtcga ggacgtcgat cgccgcctct
     3001 cctactgggc gctggcccac ccgctcgcca agcctgattt ccatcatccg gacgcctttg
     3061 cgctgcgcct ggagcacgcg acgtgagatt cggcctcgac cgcctgctgg aagagccggc
     3121 gctgcgcaag ccgctggccg ggcggcgcat cgccctgctt gcccatccgg cgtcggtcac
     3181 gcgcgacctg cggttctcgc tggacgcgct cgccgcgctg ggcgatctgc acatcgtcgc
     3241 cgccttcggt ccgcagcacg gcctgcgcgg cgacaagcag gacaacatga tcgagtcgcc
     3301 cgattaccgc gaccccgagc atggcatccc ggtgttcagc ctgtacggcg cggtgcgccg
     3361 cccgaccgcc gcgatgctgg actgcttcga cgtgctgctg gtggatctgc aggatgtcgg
     3421 ctgccgcatc tacaccttca tcacgacgtt gcgctacgtg ctggaagacg cggctcgggc
     3481 gcacaaggcg gtatgggtgc tcgaccggcc gaaccccgcc gggcgcccgg tcgaggggct
     3541 gcggctgcgt ccgggttggg aaagcttcgt cggagcgggc gcgctgccca tgcgccacgg
     3601 attgaccatg ggcgaactgg cgcgctggtt cgtgcacacc ctggcgctcg acgtcgattg
     3661 ccgcgtcgtc gagatgcagg gctgggagcc ggcgcgcgcg cccggctacg gctggccgct
     3721 cggcgagcgc gcctgggtca accccagccc gaacgcgccc aatctgtgga tggtgcgttg
     3781 ctacgccggc acggtgatgc tggagggcac cacgctgtcg gagggccgcg gcacgacgcg
     3841 gccgctggag ctgttcggcg cgcccggtct cgacgtcggc gagctgctcg cgcgcatgcg
     3901 cgccctggcg ccgcactggc tgcagggctg ccggctgcgc ccgtgctggt tcgagcccac
     3961 cttccacaag cacgccggca agctgtgcgc gggcgtgcag atccacgtgg aagacggcgc
     4021 ctacgagcac gaggcgttcc ggccctggcg cctgcaagcg ctcgccttca aggcgctcaa
     4081 gcagctgcgt cccgactacg cgctgtggcg cgacttcccg tacgaatacg aacgcaaccg
     4141 gctggcgatc gacctgatca acggcagcgc gctgctgcgc gagtgggtgg acgacgcggc
     4201 ggccacgccg gccgatctcg atgcgctggc gcagccggac gaagccgcgt ggcgcgagga
     4261 gcgcgacgcg atcctgctgt accgctgagg ccgggcgcgt cgggccgtga agcacccggc
     4321 atgctgatcg accgctggct cacccggtgg agccgcaact acgccttcct gcgccgcgcg
     4381 ctcgacgaca tcggccggca gctcgcgcgc cggccctacg acgcgatgct gcagccggac
     4441 gaggagcttt ccttcagcca gatggtgcag gacgtgcgcg tggacttcga ggtcgaagtg
     4501 ttccgcatcg acctcagcga ccgcaccatg tgggtgcgcg tcacggcgcg cagcgatctc
     4561 gccacgccgc tgcgcctcaa gccctcgctg gtgttccgca agctccccga cggccgcgcg
     4621 tacatcttcc tgtgagggcg cggctcgcac gcatgaccga gatacgcgcc gcgctcatcg
     4681 cggtgcccgc gtggcgggcc tgcgtcagtc acgctcctcg tcgaccgggt gtccaggcac
     4741 gatcttgcgg cccgtgaaca tggccgagac gaggctgccg ccttcgcgca cttcggtcac
     4801 caccaccgcc gcgacgtgca gcacgacgag cacgagcagc gcgtagaagc tgtactcgtg
     4861 gaccagcgcg aacggcttgc gcagggcgcg catgctttcg tacgcaactt ggtcgaccat
     4921 ctgcggggcg tacggcacga gtgtcactgg atcgaccccg ggcgcg
//
LOCUS       DASPGE010000044         4684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_715444, whole genome shotgun sequence.
ACCESSION   DASPGE010000044 DASPGE010000000
VERSION     DASPGE010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715444"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VD791_01450"
     CDS             <1..241
                     /locus_tag="VD791_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06669.1"
                     /translation="AEQIALGERVLERAPAHPEREEAEFILMVHYVQAARSAPDATTR
                     TRYAQRARQAAERFAARYPDSMRAAALPVLLEDLP"
     gene            complement(349..696)
                     /locus_tag="VD791_01455"
     CDS             complement(349..696)
                     /locus_tag="VD791_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06670.1"
                     /translation="MHKPLALLALALAGAASVPLLAAEGTVTWFDPTCRYFVLQLPEG
                     SEGFGLYEWKRGPEPTEGMVIAGDILGGPELTVTEKASGQEMGVVHWGDAKKQDVLVR
                     HSPDWCKSKRKRR"
     gene            799..1845
                     /gene="ltaE"
                     /locus_tag="VD791_01460"
     CDS             799..1845
                     /gene="ltaE"
                     /locus_tag="VD791_01460"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HXV06671.1"
                     /translation="MKEIDLRSDTVTKPTPGMRAAMAAAEVGDDVYGEDPTVNRLEAL
                     AAQMLGKDAALFVPSGTQSNLLGVMAHCERGDEYIVGQQAHTYKYEGGGAAVLGSIQP
                     QPLEFEPDGTLDLARVSAAIKPDDAHFARTRLLCLENTQSGKALPLDYLQRAHDFARG
                     RGLGLHLDGARVFNAAVKQAVPVREIARHFDTVSVCLSKGLGAPVGSVLCGRADLIGK
                     ARRWRKMLGGGMRQAGMLAAAGIYALTHNVERLAEDHASARALAQGLAGIDGVSVDPA
                     AVQTNMVFMTVAPERAEPLRAFLRQGNVLIGGGHQIRLVTHLDVTPADVQAVVRAVQE
                     FFATAGRQLAPQPH"
     gene            2192..3076
                     /locus_tag="VD791_01465"
     CDS             2192..3076
                     /locus_tag="VD791_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXV06672.1"
                     /translation="MAPAAAAPAAQADAAPRAAAPPPAPAKEPWYKRWFGGGAKSAQP
                     AAAQPAPAAQMPASGAPAAEGFAGDAPQRSIRTGAGQCLKTGTWDPSEGEGACPGAEP
                     VARAAPAPMAAPPAPQPVEVQPLATPQLKEERALEPEPPSLPPPPIPEPPLAAEPPPA
                     PLESQTLTLSADALFGVGSYELKPSARAALDDLVARLKEVTFQSITITGHTDPTGSSA
                     LNDRLSLQRAEEVKRYLVAHGVNAAKIETEGLGSSMPMVIERDCARLPRDKRSACYQP
                     DRRVEIEVLGASEKLAGQ"
     gene            3085..3924
                     /locus_tag="VD791_01470"
                     /pseudo
     CDS             3085..3924
                     /locus_tag="VD791_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090342.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
     gene            4075..4494
                     /locus_tag="VD791_01475"
     CDS             4075..4494
                     /locus_tag="VD791_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06673.1"
                     /translation="MSIATTTNRSLLAGLYRALDPLCEETCDAVRIACVPNGAEHRFI
                     VRIDERWRPDVYCLLAERTSVAIPADGGPLNLSAAEAHEVAQLTTVLVAERPVPERPA
                     DRPLADRPMADRVEPLPLRTPRHMPDIRGGALEHTFQ"
BASE COUNT          765 a         1635 c         1610 g          674 t
ORIGIN      
        1 cgcggagcag atcgcgctcg gcgaacgcgt cctcgagcgc gcgcctgcgc atcccgagcg
       61 cgaggaagcc gagttcatcc tgatggtgca ttacgtccag gccgcgcgca gcgccccgga
      121 cgcgacgacg cgcacacgct acgcgcagcg cgcgcggcag gcggcagagc gtttcgcggc
      181 acgctatccc gacagcatgc gcgccgcggc gctgccggtg ctgctggagg acctcccgta
      241 gcccgcatgc agcgaagcgg aatctcttcg ccggattccg ccgcccattt gttcggcgac
      301 gtatcggggc ggctgcaccc ggactacgcc gtcgtgcgcg aagtcctctt acctgcgctt
      361 gcgcttgctc ttgcaccagt ccggcgaatg gcgcaccagc acgtcctgct tcttggcgtc
      421 gccccagtgc accacgccca tctcctgacc gctggccttc tccgtcaccg tcagctccgg
      481 gccgccgagg atgtcgccgg cgatgaccat gccttccgtc ggttccgggc cccgtttcca
      541 ctcgtacaga ccgaagcctt cgctgccttc gggaagctgc agcacgaaat agcggcaggt
      601 gggatcgaac caggtgacgg tgccttcggc cgcgagcagc gggacgctcg ccgcgccggc
      661 cagggccagc gccagcaacg cgagtggctt gtgcattgcg ctcctcctcc ttcaagcgcc
      721 ggctcggacc ggcggtggcc gcattatccg tcgtttgatg ggctggtgta acatgccggg
      781 ctggtccaga gcgtgccgat gaaagaaatc gacctgcgca gcgacaccgt caccaagccc
      841 actcctggca tgcgcgcggc catggccgcc gccgaggtgg gcgacgacgt ctacggcgaa
      901 gacccgaccg tcaaccggct ggaggcgctg gcggcgcaga tgctgggcaa ggatgcggcg
      961 ctattcgtcc ccagcggcac gcagagcaat ctgctgggcg tgatggcgca ctgcgagcgc
     1021 ggcgacgagt acatcgtcgg ccagcaggcg catacctaca agtacgaagg cgggggcgcc
     1081 gccgtgctag gcagcatcca gccgcagccg ctcgagttcg agcccgacgg taccctcgac
     1141 ttggcgcgtg tgagcgctgc gatcaaaccg gacgacgcgc acttcgctcg gacgcgcctg
     1201 ctgtgcctgg agaacaccca gagcggcaag gcgctgccgc tcgactatct gcagcgcgcg
     1261 cacgacttcg cgcgcggccg cggcctggga ctgcatctgg acggcgcgcg cgtgttcaac
     1321 gccgcggtga aacaggcggt gccggtgcgc gagatcgcgc ggcatttcga cacggtgtcg
     1381 gtgtgcctgt cgaagggact gggtgctccg gtgggctcgg tgctgtgcgg gcgcgcggat
     1441 ctgatcggca aggcgcgccg ctggcgcaag atgcttggcg gcggcatgcg ccaggccggc
     1501 atgctcgcgg ccgccgggat ctacgcgctc acgcacaacg tcgagcgtct ggccgaggac
     1561 catgccagtg cgcgcgcgct ggcgcagggt ctggcaggga tcgacggcgt gtcggtggat
     1621 ccggcggcgg tacagaccaa catggtgttc atgacggtgg cgccggaacg cgccgagccg
     1681 ttgcgcgcat tcctgcgcca gggcaacgtg ctgatcgggg gcggccatca gatccgcttg
     1741 gtgacgcacc tcgacgtgac cccggccgac gtgcaagcgg tcgtgcgcgc ggtccaggag
     1801 ttcttcgcca ccgccgggcg gcagttggcg ccgcaaccgc actgaccccg caaaccggac
     1861 ccagcagccg atccgctccg ggatccggac cccggtcggc tataatccgg ccagtttttt
     1921 gcgtgccctc acaagaggat agctcatgca ttgtgctgcc agaccgattc gcttcctgtg
     1981 gctgacgtgc gcgatcgcgg ccgcattgcc ggcggcgctg ttcgcgcagg aaagaccggg
     2041 tgcggtattg ccgccgccca gcctgtacga ttccgctccg ccgccggccc aggtggcacc
     2101 cgcaccacgc gctgcggaac ccgcgccacg cgccgcggag ccggcccctg caccagccgc
     2161 ggcggagccg gcgccggtac gcgactcgca gatggcgccc gctgcagctg ccccagcagc
     2221 acaggccgac gccgcgccgc gcgcggctgc gccgcctccc gctccggcga aagagccctg
     2281 gtacaagcgt tggttcggcg gtggcgcgaa gtcggcgcag cccgcagccg cccaacccgc
     2341 accggcggcg cagatgcccg ccagcggtgc gcccgctgcc gagggctttg ccggcgacgc
     2401 gccgcagcgg agcatccgca ccggggcagg gcaatgcctg aagaccggca cctgggatcc
     2461 gtccgagggc gaaggagcat gccccggcgc cgagcccgtg gcgcgtgccg cgccggcgcc
     2521 gatggccgca ccgcccgcac cgcaaccggt cgaagtgcag ccgctcgcca cgccgcagct
     2581 caaggaggag cgcgcgctcg agcccgagcc gccgtcgctg cctccgccgc cgatacccga
     2641 gccaccgctc gccgcggagc ccccgccggc gcccctcgag tcgcagacgc tcacgctctc
     2701 ggccgacgcg ctgttcgggg tgggcagtta tgagctcaag ccgagcgccc gcgccgcgct
     2761 ggacgacttg gtggcgcgtc tcaaggaggt caccttccag tcgatcacga tcaccggtca
     2821 caccgatccc accgggtcga gcgcgctcaa tgatcgcctg tccctgcagc gcgccgagga
     2881 agtgaagcgc tacctggtgg cgcacggcgt gaacgccgcc aagatcgaga ccgagggcct
     2941 gggcagcagc atgccgatgg tgatcgagcg cgattgcgcc aggctgccgc gcgacaagcg
     3001 ctcggcctgc tatcagcccg accggcgcgt cgagatcgag gtgctcggcg ccagcgagaa
     3061 gctggccgga cagtgacgcg cgccatgcaa gcgaacggcg aaacccgcaa catggcggtg
     3121 ttctgcgact tcgagaacgt cgcgctcggc gtgcgcgacg ccaagtacga caagttcgac
     3181 atcggcaagg tgctcgagcg cctgctgctc aagggcagca tcgtggtgaa gaaggcgtac
     3241 tgcgactggg agcgctataa ggcgttcaag gcggtgatgc acgaggccgc cttcgagctc
     3301 atcgaaatcc cccacgtgcg ccagtccggc aaaaactcgg cggacatccg catggtggtg
     3361 gacgcgctcg acctgtgcta caccaaggcg cacgtggaca ccttcgtgat cgtgagcggc
     3421 gattcggact tctcgccgct ggtgtccaag ctgcgcgaga acaacaagga ggtgatcggg
     3481 gtcggcgtga agcagtcctc ctccgacctg ctgatcaaca gctgcgacga gttcatctac
     3541 tacgacgacc tggtgcgcga ggacaggagc aaggccaagc gcgcgcgcag aaagcccgcc
     3601 aggcccaagc aggcagctcg ggccgcgccc gaagcgcccg agccggaagg cgaagccgaa
     3661 gaggcggagg aaaaggacaa gtcccagcag gccatggacc tggtggtggg cactttcgag
     3721 gcgctggtgg ccgagcgcgg cgaggacgag aagatctgga gctcgatgat caagcaggcg
     3781 ctcaagcgcc gcaaacccgg tttcaacgag tcgtactacg gcttcaagag cttcaacagc
     3841 ctgctcgaag aagcgcaggc gcgcgggttg ctcaagctcg agcgcgacga gaaatccggc
     3901 ggctacgtgg tgcgtgccac cgccgcggag cccgacatcg agctggccga ctgaagcccg
     3961 gatcgggcac cgcgcccggg cgtccgccta ttgcgcgggc attcccgtct ggacagaggc
     4021 cggcctggca aggtccttgc taatacttat tgcgaaccta atcaaggggc tgcgatgagt
     4081 atcgcgacga caaccaaccg ttccctgctg gccgggctgt atcgggcgct cgatccgctg
     4141 tgcgaggaga cgtgcgatgc agtgcgcatc gcctgcgtgc ccaacggcgc cgagcaccgc
     4201 ttcatcgtgc gcatcgacga gcgctggcgc ccggacgtgt attgcctgct tgccgagcgc
     4261 accagcgtcg cgattccagc cgatggtggt ccgctgaacc tgtcggcggc ggaggcgcac
     4321 gaagtcgccc agctcaccac ggtcctggtg gcggaacgcc ccgtgccgga acgccccgcg
     4381 gatcggcccc tggccgaccg ccccatggcc gatcgagtgg agccgcttcc gctgcgcaca
     4441 ccgcgtcaca tgccggacat ccgaggcggc gcgctggaac acacgttcca gtagtcccgc
     4501 attcgtctca tcgcgctgcg ccgcggaacc gttccttgca atccaccgga gcggtgtgga
     4561 tcatcgcgta cagcttgggg cccagggcca gcagatacac ggtgttgctg ccgcctgccg
     4621 tgacgttctg cagctcgcgt gcgccggcgt cctccgagag caagtccccc tcgatgcgat
     4681 cgcc
//
LOCUS       DASPGE010000045         3601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_129579, whole genome shotgun sequence.
ACCESSION   DASPGE010000045 DASPGE010000000
VERSION     DASPGE010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129579"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="VD791_01480"
     CDS             complement(<1..1240)
                     /locus_tag="VD791_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV06674.1"
                     /translation="MQRWHEAAGRAALAAAVALATTAWADMHGGDVTHERIRDADKRP
                     GDWLTYHGTYKGWHYSGVDQINSKNVGKLRVAWVHAMPRSVRGLQSMPLAANGILYYS
                     GSYNQVFALDGTTGEVIWYYRQKLNEDLVSKQTHSPYNRGIALGLGHVYMGTLDGKLV
                     AIDMKTGKLVWETKLIDSERLTVGFTGAPLFVKDKVIIGSQGGEWPYRGPIFGVDART
                     GQEVWKFYTVGGNDGTQSDARNTWGNDTWLTGGGGGWMAGGYDADNDTVWWGTGNPAP
                     LYDWSGPDWLTAGARPGTNLYTTSVIGLDPDSGELKAYHQELPHDAWDYDSAVGEFLM
                     LERRGKKYIVHPNKSGFVFVYDRKGKVQNVWRLVKNINFVQDIKTDGTLVGRRDLTEG
                     EHKNLCPFIAGGISWNAGSYN"
     gene            complement(1501..1923)
                     /gene="dksA"
                     /locus_tag="VD791_01485"
     CDS             complement(1501..1923)
                     /gene="dksA"
                     /locus_tag="VD791_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HXV06675.1"
                     /translation="MAAEFHTPFKPYQPKKGEAYMNPKQLDHFRKILTALKEELSQDI
                     DRTVHTMQDEATIFADPNDRASQESDMALELRNRDRERKLIKKIEETITKIDAGDYGY
                     CDSCGVEIGLKRLEARPTATLCIDCKTLDELRERQVAK"
     gene            2147..2223
                     /locus_tag="VD791_01490"
     tRNA            2147..2223
                     /locus_tag="VD791_01490"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2181..2183,aa:Pro,seq:tgg)
     gene            2235..2311
                     /locus_tag="VD791_01495"
     tRNA            2235..2311
                     /locus_tag="VD791_01495"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2269..2271,aa:Arg,seq:tct)
     gene            2375..2450
                     /locus_tag="VD791_01500"
     tRNA            2375..2450
                     /locus_tag="VD791_01500"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2408..2410,aa:His,seq:gtg)
     gene            2514..>3601
                     /locus_tag="VD791_01505"
     CDS             2514..>3601
                     /locus_tag="VD791_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV06676.1"
                     /translation="MITLRKVVLQRGGKRLLDGVDLAIAPRQKIGVTGANGTGKSSLL
                     ALLRGELHAESGDVELQPGLRMAHVAQETPACDAGALEYVLDGDRELRRIERALDDAA
                     AEHTGDAGQHIDHSGHAERLAHLHADYEQHGGYTARARAAQILNGLGFRETDLERPVA
                     RFSGGWRMRLNLAQALLCPSDVLLLDEPTNHLDLDAVYWLEDWLRRYPGTVLLISHDR
                     EFLDATVSAVCHLDGRGLKLYQGDYSAFERQRAAQLATQQATYDKQQRRIAHLKSFVE
                     RFRAKATKARQAQSRLKMLARMEVVAAAQAATPFTFGFREPLPASELLLSLERARAGY
                     GADVVLQEVDLALTAGARVGLLGANGAG"
BASE COUNT          524 a         1209 c         1220 g          648 t
ORIGIN      
        1 ggttgtagct gcccgcgttc cagctgatgc cgccggcgat gaacgggcac aggttcttgt
       61 gctcgccctc ggtgaggtcg cgccgcccga ccagggtgcc gtcggtcttg atgtcctgca
      121 cgaagttgat gttcttcacc aggcgccaca cgttctgcac tttgcccttg cggtcgtaca
      181 cgaacacgaa gccgctcttg ttcgggtgca cgatgtattt cttgccgcgg cgctccagca
      241 tcaggaactc gcccacggcg gagtcgtagt cccaggcgtc gtgcggcagc tcctggtggt
      301 aggccttgag ctcgcccgaa tcggggtcga gcccgatcac cgaggtggtg tagagattgg
      361 tgccgggccg tgcgcccgcc gtcagccaat cgggcccgga ccagtcgtac agcggagccg
      421 gattgcccgt gccccaccac accgtgtcgt tgtcggcgtc gtagccgccc gccatccagc
      481 ccccgccgcc gccggtgagc caggtgtcgt tgccccaggt gttgcgcgcg tcggactgcg
      541 tgccgtcgtt gccgcccacg gtgtagaact tccacacctc ttggccggtg cgcgcgtcca
      601 cgccgaatat cgggccgcgg tagggccact cgccgccctg cgagccgatg atcaccttgt
      661 ccttgacgaa cagcggcgca ccggtgaagc cgacggtgag ccgctcggaa tcgatcagct
      721 tggtctccca cacgagcttg ccggtcttca tgtcgatcgc gaccagcttg ccgtccagag
      781 tgcccatgta gacgtgaccc agccccaggg cgatgccgcg gttgtagggc gagtgcgtct
      841 gcttcgagac caggtcctcg ttgagcttct ggcggtagta ccagatcacc tcgccggtcg
      901 tgccgtccag cgcgaacacc tggttgtacg agcccgagta gtacaggatg ccgttggccg
      961 ccagcggcat ggattgcagc ccgcgcaccg agcgcggcat ggcgtgcacc caggccacgc
     1021 gcagcttgcc gacgttcttg ctgttgatct ggtcgacgcc gctgtagtgc cagcccttgt
     1081 aagtgccgtg ataggtgagc cagtctccgg ggcgcttgtc ggcgtcgcgg atgcgctcgt
     1141 gggtcacgtc gccgccgtgc atgtccgccc aggccgtggt ggcgagcgcc acagccgcgg
     1201 ccagcgcggc gcgtccggct gcttcatgcc atcgctgcat agatgttctc ctcatggcaa
     1261 atgcatggtc cgcgtcggta agacccccgg ggcactcggt tgccaccagc accgcggcac
     1321 gggtcgttcg agcacgacac ggcgccgtcg ggcccggccg gtgcactata gtgccatccc
     1381 gccgggcgct taaaggaacg ccgcgactgc tgttatcggc cggcaaacgc cggtcaggag
     1441 ggtgaatcca cgcccgccgc aggaccggca tgcgtgtgtc tgcgcacagg gcaggcgagt
     1501 ctacttggcg acctggcgct cgcgcagctc gtcgagcgtc ttgcagtcga tgcacagggt
     1561 ggcggtgggg cgcgcttcca ggcgcttcag cccgatctcg acgccgcagc tgtcacagta
     1621 gccgtaatcg ccggcatcga tcttggtgat ggtctcctcg atcttcttga tcagcttgcg
     1681 ctcgcggtcg cggttgcgca gctcgagggc catgtccgac tcttggctgg cgcggtcgtt
     1741 cgggtcggcg aagatggtgg cctcgtcctg catggtgtgc accgtgcggt cgatgtcctg
     1801 gctcagctct tccttcagcg cggtgaggat cttgcggaag tgatcgagct gcttcgggtt
     1861 catgtaggcc tcgcccttct tgggctggta aggcttgaat ggggtgtgga actctgcggc
     1921 catcaggtga tctccccccg gagtcggttc gcggcgaagc aacaagggct cggcgagaaa
     1981 aatgcgcttt aatagcagaa tttgggtgcc cctgcaagca actttgattc cggaacgaca
     2041 cctgtacgcg ccggccgatt cgtcagactt ctgttgcaaa ccggggttcc tgcgcggcgg
     2101 ccaaaattga ccggctcggc ggccggcggg cataatgccc cccttccggg gtgtgacgca
     2161 gcctggtagc gtgcctgctt tgggagcagg atgtcggagg ttcgaatcct ctcaccccga
     2221 ccagtgtgag agccctgccc gtagctcaac cggatagagc accggccttc taagccgggg
     2281 gttatgggtt cgagtcccgt cgggcaggcc agcctcggtc gtggatcgtc cggggtcccg
     2341 gatcgtccgg tcgccccgga gcacgctcgc agtggtggct gtagctcagt tggtagagtc
     2401 ccggattgtg attccggttg tcgtgggttc gagtcccatc agccacccca tctccttccc
     2461 gcgctgccgt cggctcgatt gagcccgcgc ggcggtttgc ttcgcgtccc ggcatgatca
     2521 ccctgcgcaa ggtcgtcctg cagcgcggcg gcaagcgcct gctggacggc gtggacctcg
     2581 ccatcgcgcc gcgccagaag atcggcgtca ccggcgccaa cggtaccggc aagagcagcc
     2641 tgcttgcgct gctgcgcggg gagctgcatg ccgagagcgg cgacgtcgag ctccagccgg
     2701 gcctgcgcat ggcgcacgtg gcgcaggaaa cgcccgcctg cgatgccggc gccctcgaat
     2761 acgtgctgga cggcgaccgc gagctgcggc gcatcgagcg cgccctggac gacgccgcgg
     2821 ccgagcatac cggcgacgcg ggccagcaca tcgaccactc cgggcatgcg gagcgactgg
     2881 cgcacctgca cgcggactac gagcagcacg gcggttacac ggcacgcgcc cgcgccgcgc
     2941 aaatcctcaa tggtctgggg tttcgtgaga ccgacctcga acgcccggtt gcacggttct
     3001 cgggcggctg gcgcatgcgt ctgaacctgg cgcaggcgct gctctgcccc tccgacgtgc
     3061 tgctgctcga cgagcccacc aaccacctcg atctggacgc ggtgtactgg ctcgaagact
     3121 ggctgcggcg ctatcccggc accgtgctgc tgatctccca cgaccgcgag ttcctcgatg
     3181 ccaccgtgag cgctgtctgc cacctggacg gccgcggtct caagctgtac cagggcgact
     3241 actcggcctt cgagcgccag cgcgccgcgc agctcgccac gcaacaggcg acgtatgaca
     3301 aacagcagcg ccggatcgcg cacctgaaga gcttcgtcga gcgcttccgg gccaaggcga
     3361 ccaaagcgcg ccaggcgcaa agccggctca agatgctggc gcgcatggag gtggtggccg
     3421 ccgcgcaggc ggccacgccg ttcaccttcg gcttccgcga gccgctgccg gcctcggagc
     3481 tgctgctcag tctggagcgc gcgcgcgcgg gctacggcgc ggacgtggtg ctgcaggagg
     3541 tcgacctggc gctgaccgcc ggtgcgcgcg tgggtctgct gggcgccaac ggcgccggca
     3601 a
//
LOCUS       DASPGE010000046        10260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_481359, whole genome shotgun sequence.
ACCESSION   DASPGE010000046 DASPGE010000000
VERSION     DASPGE010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10260
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481359"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VD791_01510"
     CDS             complement(<1..662)
                     /locus_tag="VD791_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HXV06677.1"
                     /translation="MDSVDLQVLGSTAQWLDQGHRVVLATVVETWGSAPRPAGALLAV
                     RDDGQVTGSVSGGCVEDDLIDRIRRQELAGDKPELTVYGISKEEANRFGLPCGGQLRI
                     VLEPLGHKTAWLKELLARVANRELVAREVDLETGRVRLSAAGRTDTMRFDGRTLKTVH
                     GPRWRLLIIGAGQISRYLAQMAQALDYNVAVCDPREEYAHTWDLPSVELLKGMPDDVI
                     AEL"
     gene            complement(817..3024)
                     /locus_tag="VD791_01515"
     CDS             complement(817..3024)
                     /locus_tag="VD791_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXV06678.1"
                     /translation="MENVTNQSRRDFIKASAAGGGALVVGFHLPGKLAQADAGGGYAM
                     PNAWIKVGADNQVTILVARSEMGQGVATSMPMLVAEELDVDINKVKVEFAPPAEVYIN
                     SLLGGQITGGSTSVRDAWEKLRKAGASARMLLTAAAAQEWGVDAGQVRAENGNLVGPG
                     GKRMSYGMAAPKAAQMEPPKDVPLKPASAFKVVGNAKQKRLDTPMKVKGEPTFGIDTR
                     VPGMLYAAVAMSPYLGGKVKAYDDSRAKNMPGVKAVVQYSRGVAVVADSYWQAKKAKD
                     LLEIDWDEGANAGLNMQKIWGGLREASKQPGAVFREAGDADSALSGAAKTVTSTYQLP
                     FLSHSPMEPQNTTAHVTADKAVIITPTQFQQLIPHVVAGATGLKPEQVQVTTTYLGGG
                     FGRRVEVDYAIDAAEISKAAGGVPVKMVWSREDDMTHDSYRPAGLYTLTAGLDSGGKM
                     VGVRFHSTSPSISARLFPSIVKDGIDPFAVEGIDNFPYAVGNIKFTYQMHDSGVTPGY
                     WRAVSHNLNAVALECFIDECANAAGKDPIAFRLAQIDMAESKHAWSGLSAGVSVGPRI
                     KAALEMVRDKSGWGTPLAKGKGRGVAVMEGYNTVIAIVTEVTVSPNYDVALDRVVAVV
                     DAGPLVHPDQALAQMESCINYGQSACMFGEITIKDNAVQQNNFDMYRVVRMEESPKRI
                     EIHWMKPAANQPPGGLGEPGTAVVQPSIANAIFAATGKRVRTLPLTPEHIRAA"
     gene            complement(3039..3485)
                     /locus_tag="VD791_01520"
     CDS             complement(3039..3485)
                     /locus_tag="VD791_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXV06679.1"
                     /translation="MKITFNVNGKNQTVDVAPDTPLLWVLRDSLGLVGTKFGCGKALC
                     GACTVHLNNMAIRSCQTPVGSVAGQKVTTIEGLKMNNVQKAWIALDVPQCGYCQPGQI
                     MSATALLAANKNPSDSDIDAAMSGNICRCGTYTRIRAAIKAAAKMA"
     gene            complement(3708..4403)
                     /locus_tag="VD791_01525"
     CDS             complement(3708..4403)
                     /locus_tag="VD791_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HXV06680.1"
                     /translation="MSTAPMLTLCGFAASNYYNKVKLALLEKGAAFAEEQVFPSGDEN
                     TLRLTPMGKVPFLRVAGGGLSESQAIVEYLEETCPEPPLYPKDVLARAKCRELIEVLE
                     LYLEWPARRLYPQAYFGGTASEETKAEVQRALARVAKALPRLTGFETFAFGDRFTYAD
                     CAAAMHLPLARNASKLVLGQDALSDVPGLQAYLRRMAERPCVQRVNEDRKAGLEAFAA
                     YRAAKAAAAAARA"
     gene            complement(4412..5632)
                     /locus_tag="VD791_01530"
     CDS             complement(4412..5632)
                     /locus_tag="VD791_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXV06681.1"
                     /translation="MDFSFTDKVQKLQARVSAFMEQHVTPNEARFYQEIEDNRGKGNA
                     WLPTRIIEELKPLARAQGLWNLWRPLQHGGTLTNLEYAPLCEIMGRVSWAPEVFNCSA
                     PDTGNMEVLLRYGSPEQQKQWLDPLLAGEMRSAFLMTEPEVASSDATNIQCSVQRQGG
                     DYVINGRKWWSSGAGDPRCKIFILMGKSDANNPDKHKQQSMILVPANAPGVKVLRHLT
                     VFGYDDAPHGHMEVQLENVRVPAANLLLGEGRGFEIAQGRLGPGRIHHCMRIVGQAER
                     ALELMCQRLKSRVAFGKPVAEQSVWHERIAEARIGIDTARLLTLKAAYMMDTVGNKAA
                     RAEIAMIKVLAPNVAQQVIDWAIQAHGGGGVSQAFPLAAMYAHNRTLRLADGPDEVHR
                     NAIAKVELKKHDHR"
     gene            complement(5715..7151)
                     /locus_tag="VD791_01535"
     CDS             complement(5715..7151)
                     /locus_tag="VD791_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXV06682.1"
                     /translation="MQIRDKLYIGGRWVASASPATIDVHSASTEEIVARVPAGDRADV
                     DRAVAAARGAFDAWSRTPAAERAAVLSRVHDGLKARGDEIARTIATEVGTPLKMAQRI
                     QAALPVSSFGLYSGLVAEFPFEEQVGNATVVREPVGVVAAITPWNYPLHQIAAKVAPA
                     LAAGCTVVLKPSEVAPLNAFILAEIAEAAGVPAGVFNLISGTGASVGEALVQHPDVDM
                     VSFTGSTRAGRRIGELAAGTVKRVALELGGKSPSIVLDDADLAAAVKTTVNVCFLNSG
                     QTCNALTRLLVPQARYEEAARLAVENARGFTVGDPLQGDARLGPVVSAAQRERVRAYI
                     RKAVEEGAQLLEGGAEAPAGLERGYYVKPTVLGRVRPGSTVDQEEIFGPVLSIIAYRD
                     EEDAVRIANGTIYGLAAAVWSGDEARALRVARRLRAGQVDINGGGFNINAPFGGFKQS
                     GRGRELGKYGLEEFLEYKSLQRRPPKAA"
     gene            complement(7336..7776)
                     /locus_tag="VD791_01540"
     CDS             complement(7336..7776)
                     /locus_tag="VD791_01540"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXV06683.1"
                     /translation="MARGDFTFSHRLRVRWAEVDRQGIVFNGHYLTYFDVGFTEYCRA
                     IGCPYPDALLAHGSDLFAKKAALEYHAPAQYDDELDVLVRVARVGRSSVQFLIEIHRG
                     DTLLISGEIVYVNADPIARKAAPVPAFLREAIGRFERVAPQTGA"
     gene            complement(7781..8563)
                     /locus_tag="VD791_01545"
     CDS             complement(7781..8563)
                     /locus_tag="VD791_01545"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HXV06684.1"
                     /translation="MTKLPALFVSHGAPTMIIERTPAREFLAGFGAAVGRPEAVLAVS
                     AHWDTSAPAVSLAERPETIHDFFGFPQELYRLRYAAPGAPALAQRVAGLLQEAGFAVE
                     RDAARGLDHGAWVPLMLMYPQADIPVTQLSIQADLGPAHHQRLGKALAALRDENVLIL
                     ASGSATHNLSHFRRYALNDAAPDWVNDFREWVAAAVAEGRDGDLLEYRGLAPHAADNH
                     PTEEHFLPLFVALGARSPDAPGRRVHASDTYGVLAMDAYRFD"
     gene            complement(8556..9017)
                     /locus_tag="VD791_01550"
     CDS             complement(8556..9017)
                     /locus_tag="VD791_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXV06685.1"
                     /translation="MLKLELASLRELKERIGEEVVVSDWLEMTQERIDRFAQATGDHQ
                     WIHVDVERARRESPFGAPIAHGFLTLSLLSKFLNESVRFSTSKMGVNYGLNRLRFTAP
                     VPVGARIRARLKVKRVEEIEGGVQVAWDVTLEREGSDKPCLIAEWLTRRYD"
     gene            9133..10044
                     /gene="gnd"
                     /locus_tag="VD791_01555"
     CDS             9133..10044
                     /gene="gnd"
                     /locus_tag="VD791_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HXV06686.1"
                     /translation="MDIAMVGLGRMGANMARRLARGGVRVHAFDVSPMARAALADEAG
                     VASADALGPLVKALPAPRLVWLMLPAGEITQSTLDALTPLLASGDTVVDGANAYYKDS
                     MQRAQALAQQGLRFVDAGVSGGVWGLENGYAIMLGGAPEAVAHVAPFVRILAPSPDRG
                     WLHCGPAGAGHFVKMVHNGIEYGMMQAYAEGFALMAAKEPFKLDLAAVAETWRHGSVV
                     RSWLLDLSAEFLQRDARLADLKPIVADSGEGRWTALEAIELGVPAPVMSLALMARFAS
                     QGQADYANRMLAMMRNMFGGHAVQQDE"
     gene            10041..>10260
                     /locus_tag="VD791_01560"
     CDS             10041..>10260
                     /locus_tag="VD791_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HXV06687.1"
                     /translation="MIVVLMGVSGSGKTTVGEIVSRELAWPYLDADDHHPAANVEKMR
                     AGTPLTDADRWPWLDRLNELLRRRQADGD"
BASE COUNT         1510 a         3598 c         3480 g         1672 t
ORIGIN      
        1 agctccgcga tcacgtcgtc gggcatgccc ttgagcagct ccaccgacgg caggtcccag
       61 gtgtgcgcgt attcctcccg cggatcgcac accgccacgt tgtagtcgag cgcctgcgcc
      121 atctgcgcca ggtaacgcga gatctggccc gcgccgatga tcagcagccg ccagcgcggc
      181 ccgtgcacgg tcttgagcgt cctgccgtcg aagcgcatgg tgtcggtacg gccggccgcg
      241 gacagccgca cccggccggt ctcgagatcc acctcgcgcg ccaccagctc gcggttggcc
      301 acccgcgcga gcagctcctt gagccacgcg gtcttgtggc cgaggggctc gagcacgatg
      361 cgcagctggc cgccgcaggg caggccgaag cggttcgctt cctccttgct gatgccgtac
      421 accgtcagct cgggcttgtc gccggcgagc tcctgcctgc ggatgcgatc gatgagatcg
      481 tcttccacgc agcccccgga caccgagccg gtgacctggc cgtcgtcgcg cacggcgagc
      541 aaggcgccgg ccgggcgggg cgccgagccc catgtctcga cgacggtggc gagcaccacg
      601 cggtggccct gatcgagcca ctgcgccgtg cttcccagta cctgcagatc gacgctgtcc
      661 atatcgggtt gcctggaaga aggtctcgcg tgccgctggt tgtttcctgg cccgttattc
      721 ccggcgggaa ataaccctgc cgtcaaaaaa cccccgaggg gcctccccgc ccgcgggcgg
      781 agagggtctc gggggtggtt gttgccttga accgctttac gccgcgcgga tgtgctccgg
      841 cgtcagcggc agcgtgcgca cacgcttgcc ggtggcggcg aagatcgcgt tggcgatcga
      901 cggctggacg acggccgtgc cgggctcgcc cagacctccg ggcggctggt tggcggcggg
      961 cttcatccag tggatctcga tgcgcttggg cgactcctcc atgcgcacca cacggtacat
     1021 gtcgaagttg ttctgctgga cggcgttatc tttgatggtg atctcgccga acatgcacgc
     1081 cgactgcccg tagttgatgc acgattccat ctgcgccagg gcctggtccg gatgcaccag
     1141 cggaccggca tccaccaccg ccacgacgcg atccagcgcc acgtcgtagt tcggggacac
     1201 ggtcacttcg gtcacgatgg cgatcacggt gttgtagcct tccatcaccg ccacgccgcg
     1261 gcccttgccc ttcgccagag gcgtgcccca gcccgacttg tcgcgcacca tctcgagcgc
     1321 ggccttgatg cgcggaccca ccgacacgcc cgccgacaga ccggaccagg catgcttcga
     1381 ttcggccatg tcgatctggg ccaggcggaa ggcgatcggg tccttgcccg cggcgttcgc
     1441 gcactcgtcg atgaagcact ccagcgccac ggcgttgagg ttatgcgaca ccgcacgcca
     1501 gtagcccggg gtcacccccg agtcgtgcat ctggtaggtg aacttgatgt tgcccaccgc
     1561 gtacgggaag ttgtcgatgc cctccaccgc gaacgggtcg atgccgtcct taacgatgct
     1621 cgggaacagc cgcgccgaga tcgacgggct ggtcgagtgg aagcgcacgc cgaccatctt
     1681 cccgcccgaa tccaggccgg cggtcagcgt gtacagcccg gccggacggt aggaatcgtg
     1741 ggtcatgtcg tcttcgcggc tccacaccat cttcaccggc acgccgccgg cagccttgga
     1801 gatctccgcg gcgtcgatcg cgtaatccac ctccacgcga cggccgaagc caccgcccag
     1861 gtaggtggtg gtgacctgca cctgctcggg cttgaggccc gtggcgccgg ccacgacgtg
     1921 cggaatcagc tgctggaact gcgtcggcgt gatgatcacc gccttgtccg ccgtcacatg
     1981 cgccgtggtg ttttgcggct ccatcggcga gtgcgacagg aacggcagct ggtaggtcga
     2041 ggtgaccgtc ttcgccgccc cgctcagggc gctgtcggcg tcgccggcct cgcggaacac
     2101 cgcgccgggc tgcttcgagg cctcgcgcag gccgccccag atcttctgca tgttcaggcc
     2161 ggcgttcgcg ccctcatccc agtcgatctc gagcaggtcc ttggccttct tggcttgcca
     2221 gtaggagtcg gccaccaccg ccacgccgcg gctgtactgc accacggcct tgacgcccgg
     2281 catgttcttg gcgcgcgagt cgtcgtaggc cttgaccttg ccgcccaggt aggggctcat
     2341 ggccacggct gcgtacagca tgcccggcac gcgcgtgtcg atgccgaagg tgggctcgcc
     2401 cttgaccttc atcggcgtgt ccaggcgctt ttgcttggcg ttgccgacca ccttgaaggc
     2461 ggaggcgggc ttcagcggca cgtccttcgg cggctccatc tgcgccgcct tcggcgcggc
     2521 catgccgtag ctcatgcgct tgccgcccgg gccgaccagg ttgccgttct cggcccgcac
     2581 ttgcccggcg tccacgcccc actcctgggc cgcagcggcc gtcaggagca tgcgcgcgct
     2641 ggcgccggcc ttgcgcagct tctcccaggc gtcgcgcacc gaggtcgagc cgccggtgat
     2701 ctggccgccc agcaggctgt tgatgtacac ctcggcggga ggagcgaact ccaccttcac
     2761 cttgttgatg tcgacgtcga gctcctcggc caccagcatc ggcatggagg tcgcaacgcc
     2821 ctggcccatt tcggagcggg ccaccaggat ggtcacctga ttgtccgcgc ccaccttgat
     2881 ccacgcgttg ggcatggcat agccgccccc cgcgtcggcc tgggcaagct tgccaggcag
     2941 gtggaagccg accaccagcg caccgccgcc ggcggcgctg gccttgatga aatcacgacg
     3001 ggattggttc gttacgtttt ccatgtgtca cctctccctc aggccatctt cgcggccgct
     3061 ttgatggcgg cgcggattcg agtataggta ccgcagcggc agatgttgcc cgacatggcg
     3121 gcgtcgatat cggagtccga agggttcttg ttggcggcga gcagcgccgt ggcggacatg
     3181 atttgcccgg gctggcagta gccgcattgc ggaacatcga gtgcgatcca ggccttttgg
     3241 acgttgttca tcttcaggcc ttcgatcgtg gtgaccttct ggccggcgac cgagccgacc
     3301 ggggtctgac acgagcggat cgccatgttg ttcaggtgca cggtacaggc gccgcacagc
     3361 gccttgccgc acccgaactt cgtgccgacc aggcccagcg aatcacgcag gacccacagc
     3421 aacggggtgt ccggcgcaac gtcgacggtc tgattcttac cgttaacgtt gaaggtgatt
     3481 ttcataacgc tcccccttcg aggtaggttg ggatagaacg actacaggta caacacgcgg
     3541 aaggtacacc aaggggatgc ccgtttcaac cgaaaccgat ccgcgcatcg ccttgtctgg
     3601 tgcgggattg cgggcaatgc gcggttccgg agcgcggccg ggatgctgcg ttgcggcgaa
     3661 agcggctcgc ggcggatcca tcggcggcgc gcttccaggc atgcgcctca agcgcgcgcg
     3721 gctgcggccg cggccttggc ggcgcgatag gcggcgaagg cctccaggcc ggccttgcga
     3781 tcctcgttga cgcgctgcac acaaggccgc tccgccatcc tgcgcagata cgcctgcagg
     3841 cctggcacgt ccgagagcgc gtcctgaccg agtacgagct tggaggcgtt ccgcgccagc
     3901 ggcagatgca tcgcggcggc gcaatccgcg taagtgaagc ggtccccgaa cgcgaacgtc
     3961 tcgaagccgg tgagccgagg caacgccttg gccacgcgtg cgagcgcacg ctgcacctcg
     4021 gccttggtct cctcgctcgc cgtgccgccg aaataggcct gcgggtagag ccgccgcgcc
     4081 ggccactcca gatacagctc gagcacctcg atcagctcgc ggcacttggc gcgcgccagc
     4141 acgtctttcg gatacagcgg cggttcgggg caggtttcct ccaggtactc gacgatcgcc
     4201 tgcgactcgc tcaacccgcc gccggccacg cgcaggaacg gcaccttgcc catcggcgtc
     4261 aggcgcagag tgttctcgtc cccggacggg aacacctgct cctcagcgaa cgccgcccct
     4321 ttctccagca gcgcgagctt gaccttgttg tagtagttgc tggcggcgaa gccgcaaagc
     4381 gtgagcatcg gagcggtgga catgcgaggt ctcagcggtg atcgtgcttc ttcagctcga
     4441 ccttggcgat ggcgttgcgg tgcacctcgt cgggcccgtc ggccaggcgc agggtgcggt
     4501 tgtgcgcgta catcgcggcg agcggaaacg cctgggagac gccgccgccg ccgtgcgcct
     4561 ggatcgccca gtcgatcacc tgctgcgcga cgttgggcgc cagcaccttg atcatcgcga
     4621 tctcggcgcg cgccgctttg ttgcccacgg tgtccatcat gtaggccgct ttcagcgtga
     4681 gcagccgcgc cgtgtcgatg ccgatgcgcg cctcggcgat gcgctcgtgc cagacgctct
     4741 gctcggcgac cggcttgccg aacgccaccc ggctcttcag ccgctggcac atcagctcga
     4801 gcgcgcgctc ggcctggccg acgatgcgca tgcagtgatg gatgcgtccc ggaccgagcc
     4861 gcccctgcgc gatctcgaag ccgcggcctt cccccagcag caggttggcc gccggcaccc
     4921 gcacgttctc gagctgcact tccatgtggc catgcggggc atcgtcgtag ccgaacacgg
     4981 tgagatggcg cagcaccttc acgccgggcg cgttcgccgg caccaggatc atcgactgct
     5041 gcttgtgctt gtccgggttg ttcgcgtcgc tcttgcccat caggatgaag atcttgcagc
     5101 gcggatcgcc ggcgcccgag gaccaccact tgcggccgtt gatgacgtag tcgccgcctt
     5161 ggcgctggac gctgcactgg atgttggtgg cgtccgacga ggccacctcg ggctcggtca
     5221 tcaggaaggc cgagcgcatc tcgccggcga gcagcggatc gagccactgc ttctgctgct
     5281 ccggcgagcc gtagcgcagc agcacctcca tgttgccggt gtcgggcgcg gagcagttga
     5341 acacctccgg cgcccagctc acgcgcccca tgatctcgca cagcggcgcg tactcgaggt
     5401 tggtgagcgt gccgccgtgc tgcagcggtc gccacaggtt ccacaggccc tgcgcgcgcg
     5461 ccagcggctt cagctcctcg atgatgcgcg tgggcagcca ggcgttgccc ttgccgcgat
     5521 tgtcctcgat ctcctggtaa aagcgcgctt cgttgggagt gacgtgctgc tccatgaagg
     5581 ccgacacccg ggcctggagc ttctgcacct tgtcggtgaa ggagaaatcc atggtgatcc
     5641 tgtccttggt gttcctgttc gggggatgcg ggttgacgcg atgagatggg agctgccgct
     5701 tgcgtcggac ggcgtcacgc cgccttcggc gggcggcgct gcagcgactt gtactcgagg
     5761 aactcctcga gcccgtactt gcccagctcg cgcccgcggc cggactgctt gaagccgccg
     5821 aacggcgcgt tgatgttgaa gccgccgccg ttgatgtcca cctgtccggc gcgcagccgg
     5881 cgcgcgacgc gcagcgcacg cgcctcgtcg cccgaccaca cggcggccgc cagcccgtag
     5941 atcgtgccgt tggcgatgcg cacggcgtcc tcctcgtcgc ggtaggcgat gatcgacagc
     6001 accgggccga agatctcctc ctggtccacc gtcgagccgg gccgcacccg cccgagcacg
     6061 gtgggcttga cgtaatagcc gcgctcgagg ccggccgggg cttccgctcc gccttccagc
     6121 agctgcgcgc cctcctcgac ggctttgcgg atgtaggcgc gcacgcgctc gcgctgcgcc
     6181 gcggagacca cgggtcccag ccgcgcatcg ccctgcaggg gatcgcccac ggtgaagccg
     6241 cgcgcgttct ccaccgcgag ccgcgccgcc tcttcgtagc gcgcctgcgg caccagcagc
     6301 cgcgtcagcg cattgcaggt ctgccccgag ttcaagaagc agacgttgac ggtggtcttg
     6361 acggcggcgg cgagatcggc gtcgtccagc acgatcgaag gcgatttgcc gccgagctcg
     6421 agcgccacgc gcttcaccgt gcccgcggca agctcgccga tgcggcgacc ggcccgcgtc
     6481 gagccggtga aggacaccat gtcgacgtcc gggtgctgca ccagggcctc gcccacgctc
     6541 gccccggtgc cgctgatcag attgaacacg ccggccggca cgccggcggc ctcggcgatc
     6601 tcggccagga tgaaggcgtt gagcggcgcc acctcgctcg gcttgagcac cacggtgcag
     6661 cccgcggcca gcgccggtgc gaccttggcc gcgatctggt gcagcggata gttccacggc
     6721 gtgatcgcgg cgaccacgcc gaccggctcg cgcaccacgg tggcgttgcc gacctgttcc
     6781 tcgaagggga attcggccac cagcccgctg tacaggccga agctcgacac cggcagcgcg
     6841 gcctggatgc gctgcgccat cttcagcggc gtgccgactt cggtggcgat ggtgcgcgcg
     6901 atctcgtcgc cgcgcgcctt cagcccgtcg tggacgcggc tcagcaccgc ggcgcgctcg
     6961 gccgccgggg tgcgcgacca ggcatcgaag gcgccgcgcg ccgccgccac cgcccggtcc
     7021 acgtcggcgc ggtcgccggc cggcacgcgc gcgacgatct cctccgtgga ggcgctgtgc
     7081 acgtcgatgg ttgcgggaga ggcggaggcg acccatcggc cgccgatgta gagcttgtcg
     7141 cggatctgca tgggactgtc ttcctgccgg ggagagcggc gccttggtct atcgtagccc
     7201 ggatggagcg aagcggaatc cggggagcag acgattcaat ttaggggccg gatgcgagcc
     7261 cggtaatgta gccgctttcg ccgcactgcg caaatagcgc ttcggggcaa atggcgcttc
     7321 ggggcaaatg gcgcttcacg cgccggtctg cggcgccacg cgctcgaagc gcccgatcgc
     7381 ctcgcgcaga aacgcgggca ccggcgcggc cttgcgcgcg atcggatcgg cgttcacgta
     7441 cacgatctcg ccgctgatca ggagcgtgtc gccgcggtgg atctcgatca ggaactgcac
     7501 gctcgagcga ccgacgcggg ccacgcgcac cagcacgtcc agctcgtcgt cgtattgcgc
     7561 gggcgcgtga tactcgagcg cggccttctt cgcgaacagg tcgctgccgt gggcgagcag
     7621 cgcatccgga tagggacagc cgatggcgcg gcagtactcc gtgaagccga cgtcgaagta
     7681 ggtcaggtag tggccgttga acacgatgcc ctggcggtcc acttccgccc agcgcacgcg
     7741 cagccggtga ctgaaggtga agtcgccgcg cgccattcag ctaatcgaac cggtacgcat
     7801 ccatcgccag cacgccgtag gtgtcgctgg cgtgcacgcg ccgcccgggc gcgtcgggcg
     7861 accgcgcacc cagcgccacg aacagcggca gaaagtgctc ttccgtggga tggttgtcgg
     7921 ccgcgtgcgg cgccaggccc cggtactcca gcagatcgcc atcacgccct tcggccaccg
     7981 cggcggcgac ccactcgcgg aaatcgttca cccagtccgg cgccgcatcg ttcagcgcgt
     8041 agcgacggaa gtggctgagg ttatgcgtgg cgctgccgga agccaggatc agcacgttct
     8101 cgtcgcgcag agcggcgagc gccttgccca gccgctgatg atgcgccggc cccaagtccg
     8161 cctggatcga cagctgcgtc accgggatgt ccgcctgcgg atacatcagc atcagcggca
     8221 cccatgcgcc gtgatccaac ccgcgcgcgg catcgcgctc gactgcgaag cccgcctcct
     8281 gcagcaggcc cgcgacgcgc tgcgcgagcg cgggcgcgcc gggcgccgcg tagcgcagac
     8341 ggtacagctc ctgtgggaaa ccgaagaagt cgtggatggt ttccggccgc tcggcgagac
     8401 tcaccgccgg agcggaggtg tcccagtgtg ccgacaccgc cagcaccgct tcgggccggc
     8461 ccacggccgc accgaaaccg gccaggaact cgcgcgccgg cgtgcgctcg atgatcatcg
     8521 tcggcgcgcc gtgcgagacg aacagcgcag gcagcttagt catagcgtcg cgtcagccat
     8581 tcggcgatca ggcacggctt gtcgctgccc tcgcgctcga gcgtgacgtc ccaggccacc
     8641 tgcacgccgc cttcgatctc ctccacgcgc ttgaccttca ggcgcgcgcg gatgcgcgcg
     8701 cccaccggca ccggggcggt gaagcgcagc cggttcaggc cgtagttgac gcccatcttc
     8761 gaggtggaga aacgcaccga ctcgttgagg aacttcgaca gcagcgacag cgtgagaaat
     8821 ccatgcgcga tcggcgcgcc gaagggcgac tcgcggcgcg cgcgctcgac gtccacgtgg
     8881 atccactggt gatcgccggt ggcctgtgcg aagcggtcga tgcgctcctg cgtcatctcc
     8941 agccagtcgc tcaccaccac ctcctcgccg atgcgctcct tcagctcgcg caggctggcg
     9001 agctcgagct tgagcatgcc ttgtctccgg ttgtcgagaa ccccgcccgg ctgcgcaacc
     9061 gcaacgaagc tcgtcatcgc gccaactgta gcacgcggcc gagcctccgc acgccagccg
     9121 tataatgccc gcatggacat cgccatggtc ggcctggggc ggatgggcgc caacatggcg
     9181 cgccgcctgg cgcgcggcgg cgtacgcgtg cacgctttcg acgtcagccc gatggcgcgc
     9241 gcggcgctgg ccgacgaagc gggcgtcgcc agcgccgatg cgctgggacc gctggtcaag
     9301 gcgctgccgg cgccgcgcct ggtgtggctg atgctgccgg ccggcgagat cacgcagagc
     9361 acgctggacg ccctcacacc gctgctcgcc tccggcgaca ccgtcgtgga cggcgccaac
     9421 gcctactaca aggattcgat gcagcgcgcg caggcgctcg cgcagcaggg gctacgcttc
     9481 gtcgacgcgg gcgtctcggg cggcgtgtgg ggcctggaga acggctacgc catcatgctc
     9541 ggcggcgcac cggaagcggt cgcacacgtc gctcccttcg tgcgcatcct ggcgccgtcg
     9601 ccggaccgcg gctggctgca ctgcggaccg gcgggcgccg ggcacttcgt gaagatggtg
     9661 cacaacggca tcgagtacgg gatgatgcag gcctacgccg agggcttcgc cctgatggcc
     9721 gccaaggagc cgttcaagct cgatctcgcc gcggtggccg agacctggcg ccacggcagc
     9781 gtggtgcgct cgtggctgct cgatctgagc gccgagttcc tgcagcgcga cgcccgcctg
     9841 gcggacctca agccgatcgt cgccgactcc ggcgagggac gctggaccgc gctggaggcg
     9901 atcgagctgg gcgtgcccgc gccggtgatg agcctggccc tgatggcgcg cttcgccagt
     9961 cagggacagg ccgactatgc caacagaatg ctcgccatga tgcgcaacat gttcggcggg
    10021 catgcggtgc agcaggacga atgatcgtcg tgctgatggg cgtgtccggc tcgggcaaga
    10081 ccacggtcgg cgagatcgtg tcgcgcgagc tggcctggcc gtacctcgac gccgacgatc
    10141 atcacccggc cgccaacgtc gagaagatgc gcgccggcac gccgctcacc gacgccgacc
    10201 gctggccctg gctcgaccgg ctgaacgagc tgctgcggcg gcggcaggcg gacggcgaca
//
LOCUS       DASPGE010000047         4910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_598654, whole genome shotgun sequence.
ACCESSION   DASPGE010000047 DASPGE010000000
VERSION     DASPGE010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598654"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VD791_01565"
     CDS             complement(<1..870)
                     /locus_tag="VD791_01565"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HXV06688.1"
                     /translation="MIKPYKKTPFAVALVGALSIAPGASFAAGFALLEQNVSGLGNAY
                     AGSAAVAQDASTIFFNPAGLTLLPGTQAVVAGSAIYLDTEFSPTAAPVFPPFPVVTPG
                     TDTGGNLGGWSVVPNLYFSTPIGERFAVGIGVNVPFGLTTEYHDDWMGRFQGIKSELT
                     STNVNPSVAFKVSDTVSLGAGINWQKTDAELTNAVFLPAGLGVFVEGRTKLEADDDAW
                     GWNVGALFQLGSDMRVGVSYRSAIDYTLEGDVSTTFNGTPVAAGSFPAEAEAKFPDSA
                     LLSVTQRFGDKWEL"
     gene            complement(928..1332)
                     /locus_tag="VD791_01570"
     CDS             complement(928..1332)
                     /locus_tag="VD791_01570"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HXV06689.1"
                     /translation="MSDGAAHVKLPEREPVLRVVPMPADANPTGDIFGGWIMSQVDIA
                     GSIPALRLAKGRIATVAVNSFVFKQPVLIGDLVSFYAEVARVGRTSITVNVEVYAQRG
                     TQEEVTVKVTEATLTYVAVDAERHPRPVPVSV"
     gene            complement(1338..2153)
                     /locus_tag="VD791_01575"
     CDS             complement(1338..2153)
                     /locus_tag="VD791_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV06690.1"
                     /translation="MSLKEKTIFITGASRGIGRSIALRCAQDGANIVIAAKTAEPHPK
                     LSGTIHTVAREVEQAGGRALPLQLDIREEEAVFAAVKQAVGAFGGIDILVNNASAISL
                     TGTLATPIKRYDLMQAINARGTYLCSQVCLPHLLKAANPHILTLSPPLNLNPRWFAAH
                     AAYTISKYGMSMCTLGMAAEFKERGVAVNSLWPRTTIATAAVEVFFPEALASSRKPEI
                     MGDAAYLILTRDSRSATGNFYLDDEVLRAAGVTDLGRYAVTPGVPASPDIFLD"
     gene            complement(2246..4738)
                     /locus_tag="VD791_01580"
     CDS             complement(2246..4738)
                     /locus_tag="VD791_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXV06691.1"
                     /translation="MVILLWIAAFAGLLWGLAYFRASRWVWAGAAAGFLTGATLSGNL
                     PPVWRGVLWTIFVAAAGVLLVPPLRRALISDPLLGWFRKVLPQVSQTEQEALDAGTVW
                     WDGDLFSGRPDWRKLLALPKPQLSAEEQAFLDGPVEELCHMVDEWKVTHELNDLPPEI
                     WQFIKDQGFLGMIIPKEYGGLGFSALAHSQVVMKLAGRSATACVSVMVPNSLGPAELL
                     LHYGTPEQKRYYLPRLAKGLEMPCFALTGPEAGSDAGAIPDYGIVCWGEHEGKRALGM
                     RVTWEKRYITLGPVATLLGLAFKLYDPDGLLGGAKDIGITVALIPTDHPGVNIGRRHF
                     PLNASFMNGPNSGRDVFIPMDWVVGGQPYVGQGWRMLMECLAAGRSISLPSSATGGAK
                     LAARTTGAYSRIRSQFRVPIGRFEGVEEALARIGGNTYLMEAARVMTAGAVDLGEKPS
                     VVSAIVKYHCTERGRQVINDAMDVHGGKGICLGPSNYLGRGYQQVPIGITVEGANILT
                     RSMIVFGQGAIRCHPYVLKEIAATREPDRASASIAFDEALWGHVGFVVTNAARSLWLA
                     LTGARLLPAPGPAELRRHVRRLARLSASFALAADVAMLFLGGDLKRREKISARLGDVL
                     SQLYLASCVLKRYQDDGAQRTDLPFAQWALEDSEHKAQGAFFGLFHNFPVPGVGCVLG
                     LLSFPFGRRFTPPSDRLGHRVAALLLEASPARDRLTGGMFIRRHEADAVGRLELALEL
                     VAAAEAVEAKLRSAAKSGVVQGLTADARLAAGLAAGVISAQEAELWTRYNALRRACIM
                     VDDFPHDIGRNAAAEPPRVTSLQDAALQRKSA"
BASE COUNT          773 a         1750 c         1630 g          757 t
ORIGIN      
        1 cagctcccac ttgtccccga agcgttgcgt cacgctgagc agggccgagt ccggaaactt
       61 cgcttcggct tcggctggga atgacccggc cgcgaccggc gtgccgttaa aggtggtcga
      121 gacgtcgccc tccagggtgt agtcgatcgc ggaccgatag gagaccccca cccgcatgtc
      181 ggatccgagc tggaacagcg cgcccacgtt ccagccccag gcatcgtcgt cggcttcgag
      241 cttggtccgc ccctccacga agacacccag ccccgccggg aggaacaccg cgttagtgag
      301 ctcggcatcg gtcttctgcc agttgatccc cgcgcccaag ctcacggtgt cgctgacctt
      361 gaaggcgacg ctcggattga cgttggtgga cgtgagttcc gacttgatgc cttgaaagcg
      421 gcccatccaa tcgtcgtggt actcggtggt caggccgaag ggcacgttga cgcctatccc
      481 aacggcgaac cgctccccga tgggggtcga gaaatagagg ttgggtacga cggaccagcc
      541 gccgaggttg cccccggtgt cggtgcccgg cgtgacgacg ggaaagggcg gaaacaccgg
      601 cgccgccgtc gggctgaatt cggtgtccag gtagatggcc gagcccgcca ccaccgcctg
      661 cgtgcccggc agcagcgtta gaccggctgg gttgaaaaag atggtgctgg catcctgcgc
      721 gaccgctgcg ctgccggcgt aggcattgcc cagtccgctg acgttctgct cgagcagcgc
      781 gaagccagcc gcgaagctcg cgcccggcgc gatcgacaag gcccccacca gcgctaccgc
      841 gaagggtgtc ttcttgtatg gcttgatcat gacgtcctcc tcctgttgct ggcctgcaca
      901 cgacgcagag ctgcctccga ctccgcctca caccgaaacc ggcaccgggc gtggatgccg
      961 ctcggcgtcc accgccacgt aggtcaaagt ggcctccgtc accttcaccg tcacctcctc
     1021 ctgcgtcccg cgctgcgcgt acacctccac gttcaccgtg atcgaggtgc gccccacgcg
     1081 cgccacctcg gcgtagaagc tcaccagatc gccgatcagg accggctgct tgaagacgaa
     1141 cgagtttacc gccactgtgg cgatgcggcc cttggcgagc cgcagcgcgg gaatgctgcc
     1201 ggcgatgtcc acctgcgaca tgatccagcc gccgaagatg tcgccggtgg ggttggcgtc
     1261 cgcgggcatc ggcaccacgc gcagcacggg ctcgcgctcc ggcaacttca catgcgcggc
     1321 gccgtccgac atggctctca atccaagaag atgtccgggc tcgccggcac tccgggcgtc
     1381 accgcgtagc gtccgagatc cgtcaccccg gccgcgcgca gcacctcgtc gtcgaggtag
     1441 aagttgccgg tggcgctgcg gctgtcgcgc gtcaggatga ggtaggccgc atcccccatg
     1501 atctccggct tgcggctgct ggccagcgct tccgggaaga acacctccac cgcggcggtg
     1561 gcgatcgtgg tgcgcggcca cagcgagttc accgccacgc ctcgttcctt gaactccgcg
     1621 gccatgccga gcgtgcacat gctcatgccg tacttggaaa tggtgtaggc ggcgtgcgcg
     1681 gcgaaccacc ggggattgag gttgagcggc ggcgacaggg tcaggatgtg cggattggct
     1741 gccttcaaca ggtgcggaag gcagacctgc gagcacaggt aggtgccgcg cgcgttgatc
     1801 gcctgcatca ggtcgtagcg cttgatcggg gtggcgagcg tgccggtcag gctgatcgcg
     1861 ctggcgttgt tcaccaggat gtcgatgccg ccgaaggcgc cgaccgcctg cttcaccgcg
     1921 gcgaacacgg cctcctcctc gcggatgtcc agctgcagcg gcagcgcgcg gccgccggcc
     1981 tgctcgactt ctcgcgcgac cgtgtggatg gtgcccgaga gcttggggtg cggctcggcg
     2041 gtcttggccg cgatcacgat gttggccccg tcctgcgcgc agcgcagggc gatcgagcgc
     2101 ccgatgcccc ggctcgcgcc ggtgatgaag atcgtcttct ctttcaggct cacttgctac
     2161 tccccgtcgc cacagagatc acacagagag gttcgtctcg cgaatatctc cgtgtgctct
     2221 gtgtcctctg tggctggctt gtgggttacg cgctcttgcg ctgcagcgcg gcgtcctgca
     2281 gcgaggtcac gcgcggcggc tccgcggccg cgttgcggcc gatatcgtgc gggaagtcgt
     2341 ccaccatgat gcaagcgcgg cgcagcgcgt tgtagcgcgt ccacagctcc gcctcctgcg
     2401 cgctgatgac gcccgccgcg agcccggccg caaggcgcgc gtcggcggtc aacccctgca
     2461 ccaccccgga cttcgccgcg ctgcgcagct tggcctccac cgcctccgcg gcggccacca
     2521 gctccagcgc cagctcgagg cgtccgaccg catcggcctc gtgacgccgg atgaacatgc
     2581 cgcccgtcag gcggtcgcgc gcgggcgaag cctccagcag cagggccgcc acgcgatgcc
     2641 cgaggcggtc gctcggcggc gtgaaacggc gcccgaacgg gaagctgagc aggcccaaga
     2701 cgcaccccac cccgggcacc gggaagttgt ggaacaggcc gaagaaggct ccctgcgcct
     2761 tgtgctcgga gtcctccagc gcccattgcg caaacggcag atcggtgcgc tgcgcaccgt
     2821 cgtcctggta gcgcttgagc acgcacgagg ccaggtagag ctggctcagc acgtcgccca
     2881 ggcgcgccga gatcttctcg cggcgcttca agtccccgcc caggaacagc atcgccacat
     2941 ccgcggcgag cgcgaagctg gccgacaggc gcgcgagccg gcgcacgtgc cggcgcagct
     3001 cggcgggtcc gggcgccggc agcaggcgcg ccccggtgag cgccagccac agcgaacgcg
     3061 cggcgttggt cacgacgaaa ccgacgtgcc cccacagcgc ctcgtcgaag gcgatcgagg
     3121 cggaagcgcg atccggctcg cgcgtggccg cgatttcctt gaggacgtaa ggatggcagc
     3181 ggatcgcgcc ctggccgaat acgatcatgc tgcgcgtcag gatgttcgca ccctccaccg
     3241 tgatgccgat cggcacctgc tgatagccgc ggccgaggta gttgctcggc cccaggcaga
     3301 tgcccttgcc gccgtgcacg tccatggcgt cgttgatcac ctggcggccg cgttcggtgc
     3361 agtgatactt gacgatggcc gacaccaccg agggcttctc gcccagatcc accgcccccg
     3421 cggtcatcac gcgtgcggct tccatgagat aggtgttgcc accgatgcgg gcgagcgcct
     3481 cttccacgcc ctcgaacctg ccgatcggca cgcggaactg gctgcggatg cggctgtagg
     3541 cgccggtggt gcgcgcggcg agcttcgcgc cgccggtggc cgacgacggc agcgagatcg
     3601 agcggcccgc cgccaggcat tccatcagca tgcgccagcc ctgaccgacg tagggctgcc
     3661 cgcccaccac ccagtccatc ggaatgaaca cgtcgcggcc cgagttgggg ccgttcatga
     3721 acgaggcgtt gagcggaaag tggcgccgcc cgatgttgac gcccgggtgg tcggtcggga
     3781 tcaaggcgac ggtgatgccg atatccttgg cgccgccgag caggccgtcg gggtcgtaca
     3841 gcttgaaggc gaggccgagc agcgtggcca ccggccccag cgtgatgtag cgcttctccc
     3901 aggtcacgcg catgccgagc gcgcgcttgc cttcgtgctc gccccagcac acgatgccgt
     3961 agtcgggaat cgctcccgcg tcggagcccg cctccggccc ggtgagcgcg aagcagggca
     4021 tctccaggcc cttggccagg cgcggcaagt agtagcgttt ctgctccggc gtgccgtaat
     4081 gcagcagcag ctcggccggg ccgagcgagt tgggcaccat caccgacacg caggccgtgg
     4141 cgctgcggcc ggcgagcttc atcaccacct gcgagtgcgc cagcgccgag aagcccagcc
     4201 cgccgtactc cttcgggatg atcatgccga ggaagccctg gtccttgatg aactgccaaa
     4261 tctcgggcgg cagatcgttc agctcgtggg tgaccttcca ctcgtccacc atgtggcaca
     4321 gctcttcgac cgggccgtcg aggaaggcct gctcctcggc gctcaactgc ggcttgggca
     4381 gggccagcag cttgcgccag tccgggcggc cgctgaacag atcgccgtcc caccacaccg
     4441 tgcccgcatc gagcgcctcc tgctcggtct gcgacacctg cggcagcacc ttgcggaacc
     4501 agccgagcag cggatcgctg atcagcgcgc gccgcagcgg cggcaccagc agcacgcccg
     4561 ccgcggccac gaagatcgtc cacaggacgc cgcgccacac cggcggcaga ttgccgctca
     4621 acgtcgcccc ggtcagaaag ccggccgccg cgcccgccca cacccagcgc gacgcgcgga
     4681 agtacgccag gccccacagc aacccggcga aagccgcgat ccatagcaaa atcaccatcg
     4741 gattctcctg cgccgaatgc gcccttaagg cgcgacttag aaccccccca cttctcacca
     4801 cgtgggcggg gctacacgcg cgccgccggg tgccttgcac cttgcgctcg cccccgacgc
     4861 gttgtcaagc agctcgcgtt gtcaagacgc cctgcgcccg ggtcgaggta
//
LOCUS       DASPGE010000048         8339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_833623, whole genome shotgun sequence.
ACCESSION   DASPGE010000048 DASPGE010000000
VERSION     DASPGE010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8339
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833623"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VD791_01585"
     CDS             complement(<1..220)
                     /locus_tag="VD791_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06692.1"
                     /translation="MTTFVAVGVLSALLHNGPAHAQDRAVAPAGRASRVGAILEEIKA
                     RYPDPIDERRMIADAVNGVLVNLDPHSAY"
     gene            478..1260
                     /locus_tag="VD791_01590"
     CDS             478..1260
                     /locus_tag="VD791_01590"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXV06693.1"
                     /translation="MQRIANPAKERLTRGELAIGMGVRGVRGVEVARVMKTAGYDFLF
                     IDLEHGATSVETAFQISVAALDAGIAPLVRVPHGELAMGTRCLDGGGLGLVIPHVDGA
                     DEARAMVDAFKFAPLGRRSIGGAYPQFGFAPTPAAEVVSGLNDATLLVAMIETPRAVQ
                     NAEQIAAVAGIDVLLMGTNDLCLEMGIPGKLDDERVVSAIDTVVGACRKHGKWPGLGG
                     VYGRELMKRYIGRGMRFVLTGNDISLLLAAAQEQATFARGCL"
     gene            1352..2245
                     /locus_tag="VD791_01595"
     CDS             1352..2245
                     /locus_tag="VD791_01595"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXV06694.1"
                     /translation="MNVRRFGRGLGCAMLLSCVAGAAPALADSDLLRAMKAAKVVELN
                     HVWDTRSPLLSFNPPYMAALSNTHKQTTGWIPELAFASDMMYFSGQHGAPTIDAIGHI
                     SHNGKMFGGLAAEQNEQPSGLTALGIEIYPKERLVNRGVLLDVARYKKVDALAPGEEI
                     TAADLEATAKAQGVEVRAGDSLLIRTGWGKFFGDKAKYMGLRPGPGESAAKWLAARKV
                     FLTGDDQLTYEVYPEKGTVFPAHRVLIADNGIYIVENMNLEELGETLAARKNHEFVLV
                     LNPLRLRGMTGSPLNAFAIVP"
     gene            2295..2789
                     /locus_tag="VD791_01600"
     CDS             2295..2789
                     /locus_tag="VD791_01600"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXV06695.1"
                     /translation="MRSAYYLLMQTCGVLAALAIGAVALLVTGDVVARNLGLPFFPWV
                     VEVSEYSLPLATFLAAPWLLYRNEHVRLDMLLGALPRTAARLLDALADLVGIAVSLVF
                     VWYGVRVIADSRALGSMVFKTLVFPEWWLFVPVPLCFALLAVEFVRRMLGANGSARFR
                     QSTA"
     gene            2786..4105
                     /locus_tag="VD791_01605"
     CDS             2786..4105
                     /locus_tag="VD791_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXV06696.1"
                     /translation="MIWYATAALLFGCLAAVFLIGLPAAFSFFAINVVGAWYYLGGEA
                     GLAQLVRNGVAAITNFSLTPIPFFVLMGEVLFHTGVAMKAIDAIERLIRRVPGRLAVV
                     AVVAGTVFSAISGSTIATTALLGSLLLPEMLRRGYHPSMAMGPIMAIGGVDMLVPPSA
                     LAVLLGSLAGISISHLLIAGIVPGVLMAAIFVAYVVLRAKVNPALAPAFEEAPARGWS
                     RWGPLLLYVTPLVLIFVVVIAAMVAGWATPTESAAVGAVATVVVAWAYRALTLSGLMK
                     ALLGTAAISGIILFIIVGATTFSQVLTFSGATDGLVTAVRAADFGPWGVLLGMMLILL
                     ALGCFIDQVSMMLLTLPFFMPLVQHYGYDPVWFGVLYLIAMQLGLLTPPFGMLLFTMK
                     GVAPKQITMSQIFRAVTPYVVIGLVMLAVVMLFPGLAVWLPDVLLGK"
     gene            4128..6266
                     /locus_tag="VD791_01610"
     CDS             4128..6266
                     /locus_tag="VD791_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HXV06697.1"
                     /translation="MERSFKKEVALLRLGEGEVFNGEGILAVTKALLQSGVAYVGGYQ
                     GAPVSHMMDVLNDAHDILDELGVHVETNASEAGAAAMLGASINYPLRGAVTFKSTVGT
                     NVASDALSNLASAGVRGGTLIVLGEDYGEGASIIQERTHAFAMKSQMWLLDPRPDLPS
                     IVRMVQKGFELSEASSTPVMLELRIRACHVHGAFPAKDNVAPPFSRRRVLEEPDFDYG
                     RICLPPATYMQEKHKVEVRWPAAVKFIAEQGLNEFFDGPCDEVGILCQGGMYNAVIRA
                     LQQLGLADAFGAAVIPIYCLNVAYPLIPEEIARFCAGKRAVLMVEEGQPAYLEDALHT
                     VLRKADLQTKVHGKDFLPLAGEYTGEVVLTGVARWAEAMAAQGVDLGGVDASRVRAVY
                     EGLTAPKRKATEMLGAPLPVRPPGFCIGCPERPVFSAMKLVERETGKFHVSADIGCHT
                     FSTLPPFNIGNTVLGYGLGLASNSAVSPMFGKRTVTVMGDGGFWHNGLTSGVANAAFN
                     RDDAILVIMKNGYTSATGTQNIPSSLHQDANKSRDMSIERALAGLGIGWVKSITTYRV
                     AEMKKALLEAMTTGYQGLKVIIADAECQLERQRRIRPLVAEQLKRGERVVRERFGVDE
                     DVCTGDHSCIRLSGCPSLTIKPSSDPLKRDPVAHVDNGCVGCGLCGEVAHAAVLCPSF
                     FRAEIVQNPTWWDRLLERARRTVIGWMQPA"
     gene            6263..7849
                     /locus_tag="VD791_01615"
     CDS             6263..7849
                     /locus_tag="VD791_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HXV06698.1"
                     /translation="MTMQLRADQLPARPIAVLVAALGGEGGGVLSDWIVDAATAEGLP
                     VQSTSIPGVAQRTGATTYYVEIYPVRLDALGGRRPVLALTPSPGNVDVMVASELLEAG
                     RAMQNGYVSPDRTTLIASTHRIYAIAEKAAMADGRFDVERLQRAAAALAKRRVLFDMA
                     AAAAQAGTVISAVMFGAIAGAAVLPLSREACEQAIRRSGKGAEPSLRGFAIGFAAAAG
                     DAPAAATERGRVGANPVERVRSGFPAETHHILEEAVARLTDYQDRDYADLYLDRLEPV
                     VNAERAAGRAAEGYALINETGRHLALWMSYEDVIRVADLKTRRARFERVRAEADAKPH
                     EPVRIVEFLKPGVEELCAVLPPSLARPMLAWARARGKSFNAGMHVPTTSVWGFLLMRA
                     MAWLKPLRRRTSRFQEEQRLIERWLAAIRAAASVDAQLAAEIAECARLIKGYGDTHAR
                     GTGNFLRLFDTLVEGSPELAPAERAARLRQAREAALADPEGKALGQTLGGGGAKSEPA
                     VKPVVWLTPKRAETLSTDAD"
     gene            7872..8285
                     /locus_tag="VD791_01620"
     CDS             7872..8285
                     /locus_tag="VD791_01620"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXV06699.1"
                     /translation="MNKVFVHEQLVRFSHCDPAGIVYYPWFLDMAHAVKEEWFERGLE
                     HSHFKLIRERRIGTPTVNLQCDFLRPVEMGETLRWELRVAKLGNASVQLALSAKRNGE
                     ECVKLLQTVAFMNLDTRKAIPVPEDLRPRIAEYLL"
BASE COUNT         1218 a         2829 c         2961 g         1331 t
ORIGIN      
        1 agtaggccga atgcggatcc aggttgacga gcacgccgtt gaccgcgtcg gcgatcatcc
       61 gccgctcgtc gatcgggtcg gggtagcgcg ccttgatctc ctccaggatc gcacccacgc
      121 gcgacgcccg accggccggt gcgaccgccc ggtcctgggc gtgcgcgggc ccgttgtgca
      181 gcaatgcgct cagcaccccg accgcgacga aagttgtcac ccgggactcg atggcgcccc
      241 gaggcgtcac ccgatgcttt ccgatgcgtc cgttcaccgc tggcaatctc ctgtttagcg
      301 cggagtttaa cctacgggcg acgcttggcg gcgtcacccg cgcacgctct cgcaagaacc
      361 gtgctgcccc gggcgccccc tcaccccgac ccttctcccg cagcggggag agggagccct
      421 cccccgcccc gagaggcggg tagaatcgcg cggatcgctc gacaaaggga ggagacggtg
      481 caacggatcg cgaacccggc caaggaacgc ttgacgcgcg gcgagctggc catcggcatg
      541 ggcgtgcgcg gcgtgcgcgg cgtcgaagtg gcgcgggtga tgaagacggc ggggtacgac
      601 ttcctgttca tcgacctgga gcacggggct acgtcggtcg agaccgcctt ccagatctcg
      661 gtggcggcgc tcgacgcggg gatcgcgccg ctggtgcggg tgccgcacgg cgagctggcg
      721 atgggcacgc gctgcctgga cggcggtggg ctgggcctgg tgatcccgca cgtggacggc
      781 gccgacgagg cgcgcgccat ggtggacgcg ttcaagttcg cgccgctcgg gcgccgctcc
      841 atcggcgggg cctatccgca gttcggcttc gcgcccacgc cggccgccga ggtggtgagc
      901 ggcctgaacg acgccacgct gctggtggcc atgatcgaga cgccgcgcgc ggtgcagaac
      961 gccgagcaga tcgccgccgt ggcgggcatc gacgtgctgc tgatgggcac caacgatctg
     1021 tgcctggaga tgggcatccc cggcaagctc gacgacgagc gcgtggtgtc ggcgatcgac
     1081 acggtggtcg gcgcctgccg caagcacggc aagtggcccg gcctgggcgg cgtgtacggc
     1141 cgcgagctga tgaagcgcta catcggccgc ggcatgcgct tcgtgctgac cggcaacgac
     1201 atcagcctgc tgctcgccgc cgcgcaggag caggccacgt tcgcgcgcgg gtgcctgtga
     1261 ccgaaaggcg tgaccgggag gcgtgaccgg aaagcgtgac ggctgctata gttacgcgcg
     1321 tcggacaagc acaccaacag ggaggatccc aatgaacgtt cgccgcttcg ggcgcgggct
     1381 cggctgcgcc atgctcctat cgtgcgtcgc cggcgcagcg ccggccctgg ccgacagcga
     1441 tctgctgcgc gcgatgaagg ccgccaaggt ggtggagctg aatcacgtgt gggacacgcg
     1501 ctcgccgctg ctgtccttca acccgcctta catggcggcg ttgagcaaca cccacaagca
     1561 gacgaccggc tggatcccgg agctggcgtt cgcctcggac atgatgtact tcagcggcca
     1621 acacggcgcg ccgaccatcg acgccatcgg ccacatcagc cacaacggca agatgttcgg
     1681 cggcctcgcc gccgagcaga acgagcagcc cagcggactg acggcgctgg gcatcgagat
     1741 ctatccgaag gagcgcctgg tcaaccgcgg cgtgctgctc gacgtggcgc gctacaagaa
     1801 ggtggatgcg ctcgcgccgg gcgaggagat caccgccgcc gacctggagg ccaccgccaa
     1861 ggcgcagggc gtggaggtgc gcgccggcga ctcgctgctg atccgcaccg gctggggcaa
     1921 gttcttcggc gacaaggcca aatacatggg cctgcgtccc ggccccggcg agagcgccgc
     1981 gaagtggctg gcggcgcgca aggtcttcct caccggcgac gatcagctca cctacgaggt
     2041 gtacccggag aagggcaccg tgttcccggc gcaccgcgtc ctgatcgccg acaacggcat
     2101 ctacatcgtc gagaacatga acctcgagga gctgggcgag acgctggccg cacgcaagaa
     2161 ccacgagttc gtgctggtgc tcaatcccct gcgcctgcgc ggcatgaccg gttcgccgct
     2221 gaacgcgttc gcgatcgtgc cgtagcggcg atgtagtggc ggcggggcgg cggctgcacg
     2281 cgccggcaac cgacatgcgc tccgcctact acttgctgat gcaaacctgc ggcgtgctgg
     2341 ccgcgctcgc gatcggcgcg gtcgcgctgc tggtgaccgg cgacgtggtg gcgcgcaacc
     2401 tcgggctgcc ctttttcccc tgggtggtgg aggtctccga gtacagcctg ccgctcgcca
     2461 ccttcctcgc ggcgccctgg ctgctctacc gcaacgagca cgtgcggctg gacatgctgc
     2521 tcggcgctct gccgcgcact gcggcacgtc tgctcgacgc tctggcggat ctggtcggca
     2581 tcgccgtgtc gctggtgttc gtgtggtatg gcgtgcgcgt gatcgcggac tcgcgggcgc
     2641 tggggtcgat ggtgttcaag accctggtgt ttcccgagtg gtggctgttc gtcccggtgc
     2701 cgctgtgctt cgcgctgctg gcggtggagt tcgtgcggcg catgctcggc gccaacggct
     2761 cggcgcggtt ccggcagagc acggcatgat ctggtacgcc accgctgcgc tgctgttcgg
     2821 ctgcctggcc gcggtgttcc tcatcgggct cccggccgct ttctccttct tcgcgatcaa
     2881 cgtggtgggc gcctggtact acctcggcgg cgaggccggc ctggcgcaac tggtgcgcaa
     2941 cggcgtggcc gcgatcacca acttctcgct cacgccgatc ccgttcttcg tgctgatggg
     3001 cgaagtgctg ttccacaccg gcgtggcgat gaaggcgatc gacgccatcg agcggctgat
     3061 ccgccgggtg ccgggccggc tcgcggtggt ggcggtggtg gccggcacgg tgttctcggc
     3121 gatctccggc tccaccatcg ccaccaccgc gctgctcggc agcctgctgc tgccggaaat
     3181 gctgcgccgt ggctaccacc cgagcatggc gatggggccg atcatggcga tcggcggcgt
     3241 ggacatgctg gtgccgcctt ccgcgctggc ggtgctgctg ggaagcctgg ccgggatttc
     3301 gatctcgcac ctgctgatcg ccggcatcgt tcccggcgtg ctgatggcgg cgatcttcgt
     3361 cgcctacgtg gtgctgcgcg cgaaggtgaa tccggcgctg gctccggctt tcgaggaagc
     3421 gcccgcgcgc gggtggtcgc ggtgggggcc gctgctgctg tatgtcacgc cgctggtgct
     3481 gatcttcgtg gtggtgatcg ccgcgatggt ggcgggctgg gccacgccca ccgaatcggc
     3541 cgccgtcggc gcggtcgcga cggtcgtcgt cgcctgggcc taccgggcgc tgacgctgtc
     3601 gggcctgatg aaggcgctgc tcggcacggc ggcgatctcc ggcattatcc tgttcatcat
     3661 cgtgggcgcc accaccttct cgcaggtgct caccttctcg ggcgccaccg acgggctggt
     3721 gacggcggtg cgggccgccg acttcggccc ctggggcgtg ttgctcggca tgatgctgat
     3781 cctgctcgcg ctcggctgct tcatcgacca ggtgagcatg atgctgctca ccctgccctt
     3841 cttcatgccg ctggtgcagc actacggcta cgacccggtg tggttcgggg tgctgtacct
     3901 gatcgccatg cagctggggc tgctcacacc gcccttcggc atgctgttgt tcacgatgaa
     3961 gggggtggcg ccgaagcaga ttacaatgag ccagatcttc cgggcggtga ccccctacgt
     4021 ggtgatcggg ttggtcatgc tcgcggtggt gatgctgttt cccgggctgg ccgtatggct
     4081 gcccgacgtg ttgctaggca aataggcact ttgctggacg ggtagccatg gagcggtcgt
     4141 tcaagaaaga agtcgcgctg ctgcgcttgg gcgagggcga ggtcttcaac ggcgagggca
     4201 tcctcgccgt gaccaaggcc ctgctgcagt ccggggtcgc ctacgtcggc ggctatcagg
     4261 gtgcgccggt gtcgcacatg atggacgtgc tcaacgacgc gcacgacatc ctggacgagc
     4321 tgggcgtgca cgtggagacc aacgcctccg aggcgggcgc ggccgcgatg ctgggcgcat
     4381 cgatcaacta tccgctgcgc ggggcggtga ccttcaagtc cacggtcggc accaacgtcg
     4441 cctccgatgc gctttccaac ctggcatcgg ccggcgtgcg cggcggcacc ctgatcgtgc
     4501 tgggcgagga ctacggcgaa ggcgcctcca tcatccagga gcgcacgcac gccttcgcca
     4561 tgaagtcgca gatgtggctg ctcgacccgc ggccggatct gcccagcatc gtgcgcatgg
     4621 tgcagaaggg tttcgagctg tcggaggcgt ccagcacgcc ggtcatgctg gagctgcgca
     4681 tccgcgcctg ccacgtgcac ggcgccttcc cggcgaagga caacgtcgcc cccccgttct
     4741 cgcgccgccg cgtcctggag gagccggact tcgactacgg ccgcatctgc ctgccccccg
     4801 ctacttatat gcaggagaag cacaaggtgg aagtgcgctg gccggcggcg gtgaagttca
     4861 tcgccgagca ggggctcaac gagttcttcg acggcccgtg cgacgaggtc ggcatcctgt
     4921 gccagggcgg catgtacaac gcggtgatcc gcgcgttgca gcagctcggc ctggcggatg
     4981 cgttcggcgc ggcggtcatt ccgatctact gcctcaacgt cgcctacccg ctgatccccg
     5041 aggagatcgc ccgcttctgc gccggcaagc gcgccgtgct gatggtcgag gaagggcagc
     5101 ccgcgtatct cgaggacgcc ctgcacacgg tgctgcgcaa ggccgacctg cagaccaagg
     5161 tgcacgggaa ggacttcctg ccgctggcgg gcgagtacac cggcgaggtg gtgctcaccg
     5221 gcgtggcacg ctgggcggag gcgatggccg cccaaggggt cgacctgggc ggcgtggacg
     5281 cgagccgggt gcgtgccgta tacgaagggc tcaccgcacc gaagaggaag gcaaccgaga
     5341 tgctgggcgc accgctgccc gtgcgtccgc cgggcttctg catcggctgc cccgagcggc
     5401 ccgtgttctc ggcgatgaag ctggtcgagc gcgagaccgg caagttccac gtctcggcgg
     5461 acatcggctg ccacaccttt tcgacgctgc cgccgttcaa catcggcaac accgtgctcg
     5521 gctacggcct gggcctggcg tcgaactcgg cggtgagccc gatgttcggc aagcgcaccg
     5581 tcacggtgat gggcgacggc ggcttctggc acaacggcct cacctcgggc gtggccaacg
     5641 ccgccttcaa ccgcgacgac gccatcctgg tgatcatgaa gaacggctac acctcggcca
     5701 ccggcaccca gaatatcccg tcatcgctgc accaggacgc gaacaagtcg cgcgacatgt
     5761 cgatcgagcg cgcgctggcg ggcctgggca tcggctgggt gaagagcatc accacctatc
     5821 gagtcgcaga gatgaagaaa gcgctgctcg aggcgatgac caccggctac cagggtctga
     5881 aggtgatcat cgccgacgcc gagtgccagc tcgaacggca gcggcgcatc cgcccgctgg
     5941 tcgcggagca gctcaagcgc ggcgagcgcg tggtgcgcga gcgcttcggc gtggacgagg
     6001 acgtgtgcac cggcgaccac tcctgcatcc gcctgtccgg ctgtccctcg ctcacgatca
     6061 agcccagctc cgatccgctc aagcgcgacc cggtcgccca cgtcgacaac ggctgcgtcg
     6121 gctgcggcct gtgcggcgaa gtagcgcacg cggcggtgct gtgcccgtcg ttcttccgcg
     6181 ccgagatcgt gcagaacccg acgtggtggg atcgcctgct ggagcgcgca cggcgcaccg
     6241 tgatcggctg gatgcagccc gcatgaccat gcagcttcgc gccgaccaac tgccggcccg
     6301 cccgatcgcg gtcctcgtcg ccgcgctcgg cggcgagggc ggcggcgtgc tgtcggactg
     6361 gatcgtggat gcggccaccg ccgaggggtt gccggtgcag agcacctcga tccccggcgt
     6421 tgcgcagcgc accggcgcga cgacttatta tgtggagatc tacccggtgc gcctcgacgc
     6481 gctcggcggc cggcggccgg tgctggcgct gacgccctcc cccggaaacg tggacgtgat
     6541 ggtggcctcg gagctgctcg aagccggccg cgccatgcag aacggctacg tcagcccgga
     6601 tcgcaccacg ctgatcgcct ccacgcatcg catctacgcg atcgcggaga aagcggcgat
     6661 ggccgacggc cgcttcgatg tcgaacgcct gcagcgggcc gcggcggcgc tcgcgaagcg
     6721 ccgcgtgctg ttcgacatgg ccgccgcggc ggcgcaggcg ggcacggtga tcagcgcggt
     6781 catgttcggt gcgatcgccg gcgccgccgt cctgccgctg tcgcgagagg cctgcgagca
     6841 agccatccgg cgttcgggca agggcgccga gccgagcctg cgcggcttcg cgatcgggtt
     6901 tgcggcggca gcgggggatg caccggccgc agcaaccgag cgcgggcgag ttggcgccaa
     6961 tccggtcgag cgggtgcgca gcggctttcc ggccgagacg caccatatcc tcgaggaggc
     7021 ggtggcgcgc ttgaccgatt atcaggatcg cgactacgcc gacctgtatc tcgaccgcct
     7081 ggaacccgtc gtgaacgccg agcgtgctgc gggccgcgcc gccgagggct acgcgctgat
     7141 caacgaaacc ggccgccacc tggcgctgtg gatgagctac gaggacgtga tccgggtggc
     7201 cgatctcaag acccgccgcg cgcgcttcga gcgcgtgcgc gccgaggccg acgccaagcc
     7261 gcacgagccg gtgcgcatcg tcgagttcct caagccgggc gtggaggagc tgtgcgccgt
     7321 gctgccgcct tcgctcgcgc gcccgatgct ggcctgggcg agagcgcgcg gcaagtcgtt
     7381 caacgccggc atgcacgtgc ccaccaccag cgtgtggggc ttcctgctga tgcgcgcgat
     7441 ggcctggctc aagccgttgc gccggcgcac ctcgcgcttt caagaggagc agcggctgat
     7501 cgagcgctgg ctcgccgcga ttcgcgctgc ggcttccgtc gatgcgcagc tcgccgcgga
     7561 gatcgccgag tgcgcccgcc tgatcaaggg ctacggcgac acgcacgcgc gcggcacggg
     7621 caacttcctg cggctgttcg acacgctggt ggaaggctcg ccggagctgg cgcccgccga
     7681 acgcgcagcg cggttgcgcc aggcgcgaga agcggcgctg gccgaccccg aaggcaaggc
     7741 tttgggacag acgctgggtg gcggcggcgc caaatccgag cccgcggtca agccggtggt
     7801 gtggctgacg ccgaagcgcg ccgaaaccct gtccaccgac gccgactgac ccgctcttcc
     7861 ccgcccgcac cgtgaacaaa gtcttcgtgc acgagcagct cgtgcgcttc tcccactgcg
     7921 accccgcagg gatcgtctac tacccctggt tcctcgacat ggcgcacgcc gtgaaagagg
     7981 agtggttcga gcgcgggctc gagcactcgc acttcaagct catccgggag cggcgtatcg
     8041 gaacgcccac cgtcaacctg caatgcgatt tcctgcgccc ggtggagatg ggcgagacgc
     8101 tgcgctggga gctgcgcgtc gccaagctgg gcaacgcctc ggtacagctc gcgctcagcg
     8161 ccaagcgcaa cggcgaggag tgcgtgaagc tgctgcagac cgtcgccttc atgaacctcg
     8221 acacgcgcaa agccatcccc gtgcccgagg acctgcgccc gcgcatcgcg gagtacctgc
     8281 tctaagcccg ttccgcgaag agccgcaggg tcgtcggggg gggacgattc gcgcctgcg
//
LOCUS       DASPGE010000049         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1301835, whole genome shotgun sequence.
ACCESSION   DASPGE010000049 DASPGE010000000
VERSION     DASPGE010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301835"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VD791_01625"
     CDS             <1..629
                     /locus_tag="VD791_01625"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HXV06700.1"
                     /translation="FDVADGPEIETDWHNFTALNQPENHPARSMHDTFYVEGGLLLRT
                     HTSSMQVRYARQHQPPIRVIVPGRTYRVDSDATHSPMFHQVEGLWLDEHISFADLKGV
                     YTDFLQRFFETDALRVRFRPSFFPFTEPSAEIDMAFDSGPLEGRWLEISGAGQVHPNV
                     VRNFGLDPERWIGFAFGSGLERLAMLRYGIDDLRLFFEGDLRFLRQFA"
     gene            634..>2093
                     /gene="pheT"
                     /locus_tag="VD791_01630"
     CDS             634..>2093
                     /gene="pheT"
                     /locus_tag="VD791_01630"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HXV06701.1"
                     /translation="MQFSERWLRELTDPPLAIAELTHRLTMSGLEVEFCEAVLDDHVL
                     LIKLTPNRADCLSVLGVAREVAALTGAPLKPPPVAPVAPRCDAQFPVRISDPDGCGRF
                     TARVIRNVNPAAPTPEWMRQRLERSGLRSISALVDVTNYVMLELGRPLHVYDLDKLSG
                     GIDVRFGRSGERVTLLNEQIVALEPDVLAITDASGPIGLAGIMGGDSTKADSGTRHVL
                     LEAAFFYPEAIAGRARRYNFTSDASHRFERGVDFDNNVAGIERATRLILDICGGEPGP
                     VRDTVARLPQRKPVRMRVERARKIIGLPIAAEEMSDIFSRLGLAWRAEGQGAAQSFLV
                     TPPSYRFDLEIEEDLIEEVARLHGFERIPARPPLARAAMRARPETQRTLHELRGRMAA
                     RGYHEVVNFSFVEPAWEEDLAGNAQPIRLMNPIASQLSVMRSSLIGGLVDKVRYNLNR
                     RLDRVRLFEVGRVFLREPGAPEGALDVAGIRQPTRVG"
BASE COUNT          302 a          745 c          719 g          327 t
ORIGIN      
        1 gcttcgatgt ggccgacggc ccggagatcg agaccgactg gcacaacttc accgcgctca
       61 atcagccgga gaatcatccg gcgcgctcca tgcacgacac gttctacgtg gaaggcggcc
      121 tgctgctgcg cacgcacacc agttccatgc aggtgcgcta cgcgcgccag caccagccgc
      181 cgatccgggt catcgtgccg gggcgcacct accgggtgga cagcgacgcg acgcactcgc
      241 ccatgttcca tcaggtcgag ggactgtggc tcgacgagca catcagcttc gccgatctga
      301 agggcgtgta caccgacttc ctgcagcgct tcttcgaaac cgatgccctg cgcgtgcgct
      361 tccggccgtc cttcttcccg ttcaccgagc cctcggccga gatcgacatg gccttcgaca
      421 gcggcccgct cgaggggcgt tggctggaga tctcgggcgc cggccaggtg caccccaacg
      481 tggtgcgcaa cttcggcctc gaccccgagc gctggatcgg cttcgccttc ggcagcggcc
      541 tggagcggct cgccatgctg cgctatggca tcgacgacct gcgcctgttc ttcgagggcg
      601 acctgcgttt cctgaggcag ttcgcctgat cccatgcagt tctccgaacg ctggctgcgc
      661 gagctcaccg atccgcctct ggccatcgcc gagttgacgc accggctcac catgtcgggc
      721 ctggaggtgg agttctgcga ggcggtgctg gacgaccacg ttctgctgat caagctgacg
      781 ccgaaccgcg ccgattgcct gtcggtgctc ggcgtcgcgc gcgaggtggc ggcgctcacc
      841 ggggcgccct tgaagccgcc gccggtcgcg ccggtcgctc cccgctgcga tgctcagttc
      901 ccggtgcgca tctccgaccc cgatggctgc gggcgcttca ccgcgcgcgt gatccgcaac
      961 gtgaatcccg ccgcgccgac acccgagtgg atgcggcaac gcctggagcg ctcgggcctg
     1021 cgctcgatct cggcgctggt ggacgtcacc aactacgtga tgctggagct gggccgcccg
     1081 ctgcacgtgt acgacctgga caagctctcc ggcggcatcg acgtgcgctt cggccgcagc
     1141 ggcgagcgcg tgacgctgct caacgagcag atcgtggcgc tcgagcccga cgtgctcgcc
     1201 atcacggacg cgtcggggcc gatcggcctg gcgggcatca tggggggcga cagcaccaag
     1261 gccgattccg gcacacgcca cgtgctgctg gaagccgcct tcttctaccc cgaggccatc
     1321 gccgggcgcg cgcgccgcta caacttcacg agcgacgcgt cgcaccgctt cgagcgcggc
     1381 gtggacttcg acaacaacgt cgccgggatc gagcgcgcca cacggctgat cctggacatc
     1441 tgcggcggcg agcccgggcc ggtgcgcgac acggtggcgc gcctgccgca gcgcaagccg
     1501 gtgcgcatgc gcgtggagcg ggcgcgcaag atcatcgggc tgccgatcgc ggccgaggag
     1561 atgagcgaca tcttctcgcg gctcgggctg gcctggcgcg ccgaggggca gggcgcggcg
     1621 cagtcgttcc tggtgacccc tcccagctac cggttcgacc tggagatcga agaggacctg
     1681 atcgaggaag tggcgcggct gcacggcttc gagcgcattc ccgcccgtcc gcccctcgcc
     1741 cgcgcggcga tgcgcgcccg ccccgagacg caacgcacgc tgcacgagct gcgcggccgg
     1801 atggcggccc gcggctatca cgaagtggtg aacttcagct tcgtggagcc cgcctgggag
     1861 gaggatctcg ccggcaacgc ccagccgatc cgattgatga acccgatcgc gagccagctc
     1921 tcggtgatgc gtagctccct gatcggcggg ctggtggaca aggtgcgcta caacctgaac
     1981 cgtcgcctgg accgggtgcg gctgttcgaa gtgggccgcg tgttcctgcg cgagcccggc
     2041 gcgcccgagg gcgcgctcga tgtcgccggc atccgccagc cgacgcgggt ggg
//
LOCUS       DASPGE010000050         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_950477, whole genome shotgun sequence.
ACCESSION   DASPGE010000050 DASPGE010000000
VERSION     DASPGE010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950477"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..2254
                     /gene="recQ"
                     /locus_tag="VD791_01635"
     CDS             443..2254
                     /gene="recQ"
                     /locus_tag="VD791_01635"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HXV06702.1"
                     /translation="MPSPLDLLRTVFGYAEFRGEQAQIIDHVVAGGDALVLMPTGGGK
                     SLCYQIPAMLRAGVGIVVSPLIALMQDQVDALLQLGVRAAFLNSSLEADAAREVQRRL
                     FVGDLDLLYVAPERLLTDSFLAMLEGLRARHGLALFAIDEAHCVSQWGHDFRPEYIQL
                     SVLPERFPGIPRIAMTATADAITRAEIVDKLELAQARRFVASFDRPNIRYHVVEKDNP
                     RLQLLDFLAGHGGQAGIVYCLSRRKVDETAEWLREHGIDALPYHAGMEQQQRRDNQQR
                     FQREDGVVVVATIAFGMGIDKPDVRFVAHLDLPKSLEGYYQETGRAGRDGEPAEVWLA
                     YGLADVVLFKSWIEESEAPPEQKRIESMKLDAMLGYCEAASCRRVVLLNYFGEAARPC
                     GNCDVCLEPPQTWDATVAAQKLFSAVLRTGSRFGAGHLIDILRGKATERVEQLGHDRL
                     PTFGVGADLDDLAWRSVVRQLIALGLLHSDARRYGALRLTDAARPVLRGELPLKLRRA
                     RPKHQRKGRRGEQRAATLVAQGLDVDLVEALRTLRRELAGAQRVPPYVIFHDATLHEL
                     ARVRPRTEPELYLVSGIGANRVQRYGQKLLEVLRRFD"
     gene            2338..>2604
                     /locus_tag="VD791_01640"
     CDS             2338..>2604
                     /locus_tag="VD791_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HXV06703.1"
                     /translation="MSLAVLHTRAISGMDAPLVTVEVHLGGGLPNFTIVGLAETEVKE
                     ARDRVRAALQNARFEFPARRITVNLAPADLPKESGRFDLPIALGI"
BASE COUNT          403 a          906 c          885 g          410 t
ORIGIN      
        1 cgtcacgctg atcatcaacg cctttcactg cgcccccaac cccttcgcca agaaggacgc
       61 ctgcggcgcc gacgcggtcg cgaccatcaa gcgcagcgac ttcggcatga ccacgatgct
      121 gcccggcctg ggcgacgagg tgaagctgct gatcaacatc gaagcattca aggattgagc
      181 ccggagaggg ggctagcgga agcacaccac accgccctgc cggcgcagta gattcccctc
      241 ctcttcgagg aggggtggcg cgcgaagcgc gacggggtgg ttcagccacg ctcacaccac
      301 accaccccgc ctcgtgagag gtgtggagcg tctttttagg tcgcgccttc agggcgcata
      361 aggcgtggca cgcggagcgt gacggggtgg ttcacccgcc tcgcgcctct gcgcctcggc
      421 atggccgata atagtcccgg ccatgccctc ccccctcgat ctgctgcgca ccgtcttcgg
      481 ctacgccgag ttccgcggcg agcaggcgca gatcatcgac cacgtcgtcg ccggaggcga
      541 tgcgctggtg ctgatgccca ccggcggggg caaatcgctg tgctaccaga tccccgccat
      601 gctgcgcgcc ggcgtgggca tcgtcgtttc gccgctgatc gcgctgatgc aggaccaggt
      661 ggacgcgctg ctgcagctcg gcgtgcgcgc cgctttcctc aactccagcc tcgaagccga
      721 cgccgcacgt gaggtgcagc gccgcctgtt cgtcggcgac ctcgacctgc tgtacgttgc
      781 gcccgagcgc ctgctgacgg acagcttcct cgccatgctg gagggtctgc gcgcgcgcca
      841 cgggctggcg ctgtttgcca tcgacgaggc gcactgcgtg tcgcaatggg gccatgattt
      901 ccgccccgag tacatccagc tctccgtgct gccggagcgc ttccctggca tcccgcgcat
      961 cgcgatgacc gccacggccg atgcgatcac gcgcgccgag atcgtggaca agctcgagct
     1021 ggcgcaggcg cgccgtttcg tcgccagctt cgaccgcccg aacatccgct atcacgtggt
     1081 ggagaaggac aacccgcgct tgcagctgct cgacttcctc gccggccacg gcggacaggc
     1141 cggcatcgtc tattgcctgt cgcgccgcaa ggtggacgaa accgcggagt ggctgcgcga
     1201 gcacggcatc gatgcgctgc cgtaccacgc cggcatggag caacagcagc gccgcgacaa
     1261 ccagcagcgc ttccagcgcg aggacggcgt ggtggtggtg gccaccatcg ccttcggcat
     1321 gggcatcgac aagccggacg tgcgcttcgt cgcccacctg gacctgccca agagcctgga
     1381 gggctactac caggagaccg ggcgtgcagg gcgcgacggc gagccggccg aagtctggct
     1441 cgcgtacggg ctggccgacg tggtgctgtt taagagctgg atcgaggaat cggaggcgcc
     1501 gcccgagcag aagcgcatcg agagcatgaa gctcgacgcc atgctcggtt attgcgaggc
     1561 cgcgagctgc cggcgtgtgg tgctgctcaa ctacttcggt gaggccgcgc ggccctgcgg
     1621 caactgcgac gtgtgcctgg agccgccgca gacctgggac gcgacggtgg cggcgcagaa
     1681 gctgttttcg gccgtgctgc gcaccggcag tcgcttcggc gcgggccacc tgatcgacat
     1741 cctgcgcggc aaggccacgg agcgcgtcga gcagctcggc cacgatcgcc tgccgacctt
     1801 cggcgtgggc gccgacctcg acgacctggc atggcgcagc gtggtgcggc agctgatcgc
     1861 gctcgggctg ctgcattcgg atgcgcggcg ctacggcgcg ctgcggctca ccgatgcggc
     1921 gcggcccgtg ctgcgcggtg aattgcccct gaagctgcgt cgcgccaggc cgaagcacca
     1981 gcgcaaggga cgtcgcggag agcagcgcgc ggcgacgctc gttgctcagg gcctggacgt
     2041 cgacctggtc gaagcattgc gcactctgcg ccgcgagctc gccggcgccc agcgcgtacc
     2101 gccctatgtc atcttccacg acgctacgct gcacgagctg gcgcgcgtgc gcccgcggac
     2161 cgaacccgag ctctatctgg tctccggcat cggcgcgaat agagtgcagc gctacgggca
     2221 gaagctgctg gaagtcctgc gccggttcga ctgatagaac agcctcacct tcgacgggag
     2281 agggttgggg tgtcagggtg tacactcctg cggcattctt tcgccgccct tgcagacatg
     2341 tctctcgccg tcctccacac ccgcgccatc tccggcatgg acgcgccgct ggtgacggtg
     2401 gaagtgcacc tgggcggcgg gctgcccaac ttcaccatcg tgggcctggc cgaaaccgag
     2461 gtaaaggaag cgcgcgaccg cgtgcgcgcc gcgctgcaga acgcgcgctt cgagttcccc
     2521 gcgcggcgca tcacggtcaa cctcgcgccc gctgacctgc cgaaggaaag cggccgcttc
     2581 gacctgccga tcgcgctggg gatc
//
LOCUS       DASPGE010000051         3687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1653862, whole genome shotgun sequence.
ACCESSION   DASPGE010000051 DASPGE010000000
VERSION     DASPGE010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653862"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VD791_01645"
     CDS             <1..601
                     /locus_tag="VD791_01645"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HXV06704.1"
                     /translation="AGFFVIFLAGLSWRILIGLTAGATACLPLMWSMLHDYQRQRVLT
                     LLDPSQDPLGAGYHTIQAAIALGSGGFFGKGWLNGTQAHLDFLPERSTDFIFAVFSEE
                     FGLFGNLILVTLFLFVIGRGMFISFNASTLFERLTAGAITLTFFTYVAVNIGMVSGVL
                     PVVGVPLPLISYGGTSLVSLLFGMGILMSIHTHKRLVRT"
     gene            610..1548
                     /locus_tag="VD791_01650"
     CDS             610..1548
                     /locus_tag="VD791_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HXV06705.1"
                     /translation="MRATRREAARGALAIAAAAVSLLLSACGGAPHRPERAEPPRKPG
                     GYYLDDGPHANPPADLDKVPDAQPRWEPIKTSTARPYTALGRSYTPMTALAPYSETGM
                     ASWYGKRYHGQPTASGEIYDMYGMTAAHPTLPIPSYARVTHLASSKIVVVRINDRGPF
                     HADRLIDLSYTAAHRLGMLSEGSARVRVQSILPEQMQPVVASAPPPPSMDRVDGVDSV
                     DGVDSAGAAPVRVQTAPAQGVYLQLGAFASADNAQAFLRRMGTELSWLAGIGAIHHDG
                     RLYRVQAGPYATRDEALDAAGRIDRTLDLKPLIVTR"
     gene            1566..2501
                     /locus_tag="VD791_01655"
     CDS             1566..2501
                     /locus_tag="VD791_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HXV06706.1"
                     /translation="MHTLICGSIAYDTIMVFRDRFKNHILPDQIHILNVAFLVPDMRR
                     EFGGCAGNIAYNLRMLGGSPLIMATVGEDFQPYAYRLERLGLAQTHVRQVKDSYTAQA
                     FITTDLDDNQLTAFHPGAMNFSHQNKVSDARDVSLGIVAPDGREGMLNHAREFSEAGI
                     AFIFDPGQGLPMFSGDELLVMVRQSTCVAVNGYEGRLLQERIGMPLEKLAGEVKALVV
                     TQGAEGSTVYTGGQILRIPSVPPARVVDPTGCGDAYRAGLLYGMAQGYDWPTTGRLAS
                     LLGSIKVASRGAQNHQLSRDEIGARYRNAFGSSLW"
     gene            2543..3361
                     /locus_tag="VD791_01660"
     CDS             2543..3361
                     /locus_tag="VD791_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXV06707.1"
                     /translation="MRASAPPAAASPLVQGWRLLRLIVHLVRGLATEALVFPFVGSER
                     EFAIIRRWSRGLLRVLNVKLRVSGTPPGGRTPTLLVTNHISWLDIWLIHAVCPVRFVA
                     KSDVRRWPVLGWLVARSGTIFIERTRRHDTGRTNRRIVEVLSRGERVGVFPEGTTTDG
                     THLRPFHASLFQPALGAGARIVNAAIRYPLHDGAPNLDVAYTGERSLLASLRLILAQP
                     RLHAELIFCGVLEPVGKTRRDLARESHRLIAHALGLSRPGTEPETADGPPDAEP"
     gene            complement(3282..>3687)
                     /locus_tag="VD791_01665"
     CDS             complement(3282..>3687)
                     /locus_tag="VD791_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HXV06708.1"
                     /translation="CAATYRSFLARMYGNQPQRWSDALAGHDRLRVIVNAMTRMRLCT
                     AEGEMEFAHKGRPKRLPAGYLPWFDVPERRSRDTPLIFGHWSALGLIKRPDLFGLDTG
                     CLWGRRLTALRLEDRRLFQVRCRDAKAPARAR"
BASE COUNT          552 a         1313 c         1232 g          590 t
ORIGIN      
        1 ggccggcttc ttcgtgatct tcctcgccgg gctgagctgg cgcatcctga tcggactgac
       61 cgccggggct acggcctgcc tgccgctgat gtggtccatg ctgcacgatt atcagcgcca
      121 gcgcgtgctc acgctgctcg atcccagcca ggacccgctc ggcgccggct atcacacgat
      181 ccaggccgcc atcgcgctgg gatcgggcgg gttcttcggc aagggctggc tgaacggcac
      241 gcaggcgcat ctcgacttcc tgcccgagcg cagcaccgac ttcatcttcg cggtgttctc
      301 cgaggagttc ggtctgttcg gcaatctgat cctggtcacc ttgttcctgt tcgtgatcgg
      361 gcgcggcatg ttcatctcct tcaacgcctc taccctgttc gagcggctca ccgccggggc
      421 catcacgctg acgttcttca cctacgtggc ggtgaacatc gggatggtga gcggcgtgct
      481 gccggtggtc ggggtgcccc tgccgctgat cagctacggc ggcacctcgc tggtgagcct
      541 gctgttcggc atgggcatcc tgatgagcat ccatacgcac aagcgactgg tgagaacatg
      601 aggctcgcgg tgcgagccac gaggcgcgag gccgcgcgcg gcgccttggc catcgcagcg
      661 gcggcggtct cgctgctgct gagcgcctgc ggcggcgctc cccaccgtcc ggaacgcgcg
      721 gagccgccgc gcaagccggg cggctattac ctcgacgacg ggccgcacgc caatccgccg
      781 gcggatctgg acaaggtgcc cgatgcgcag ccgcgctggg agccgatcaa gacctccacc
      841 gcgcgcccct acaccgcgct gggccgcagc tacacgccga tgacggcgct cgccccgtac
      901 agcgagacgg gcatggcctc ctggtacggc aagcgctacc acgggcaacc gaccgcatcg
      961 ggcgagatct acgacatgta cggaatgacc gctgcgcatc cgacgctgcc cattcccagc
     1021 tacgcgcgcg taacgcatct ggcgagcagc aagatcgtgg tggtgcgcat caacgaccgc
     1081 gggccgttcc atgccgatcg gttgatcgac ctgtcgtaca cggcggctca ccggctcggc
     1141 atgctgagcg agggcagcgc gcgggtgcgg gtgcagagca tcctccccga gcagatgcag
     1201 ccggtcgtgg caagtgcgcc accgccgccg tcgatggaca gggtggacgg cgtggacagc
     1261 gtggacgggg tggacagcgc gggcgccgcg ccggtgcgcg tgcagaccgc cccggcccag
     1321 ggcgtgtatt tgcagctcgg cgcattcgct tcggcagaca acgcgcaggc gtttctgcgc
     1381 cgcatgggca ccgagctttc ctggcttgcc ggaatcggcg ccatccatca cgacggccgc
     1441 ctgtaccggg tgcaggccgg tccctatgcc acgcgcgacg aggcgctgga tgccgccggc
     1501 cgcatcgacc gcacgctcga tctgaagccg ctcatcgtca cccgctgact cgcgagaccc
     1561 ctgccatgca tacgctgatc tgcggctcca tcgcctacga caccatcatg gtgttccgcg
     1621 accgcttcaa gaaccacatc ctgcccgatc agatccacat cctgaacgtg gcgttcctgg
     1681 tgccggacat gcgccgcgag ttcggcggct gcgccggcaa catcgcctac aacctgcgca
     1741 tgctcggcgg cagcccgctg atcatggcca ccgtcggcga ggatttccag ccctatgcct
     1801 atcgcctcga gcgcctgggc ctggcgcaga cgcacgtgcg ccaggtgaag gacagctaca
     1861 ccgcgcaggc cttcatcacc accgatctcg acgacaatca gctcaccgcc ttccaccccg
     1921 gggcgatgaa cttctcgcac cagaacaagg tgagcgatgc gcgcgacgtg agcctgggca
     1981 tcgtcgcccc ggacggccgc gagggcatgc tcaaccatgc gcgcgagttc agcgaggccg
     2041 gcattgcgtt catcttcgat ccggggcagg gcctgcccat gttcagcggc gacgagctgc
     2101 tcgtcatggt gcgccagtcg acctgcgtgg cggtcaacgg ctacgagggc cggctgctgc
     2161 aggagcgcat cggcatgccc ctggagaagc tggccggcga ggtgaaggcg ttggtggtga
     2221 cgcagggcgc ggaaggctcc accgtctaca ccggcggcca gatcctgcgc atcccgagcg
     2281 tgccgccggc gcgcgtggtg gatccgaccg gctgcggcga tgcctaccgg gcggggctgc
     2341 tgtacgggat ggcgcaggga tacgactggc ccaccaccgg ccgcctcgcc tcgctgctgg
     2401 gttcgatcaa ggtcgccagc cgcggcgcgc agaaccatca actttcccgc gacgaaatcg
     2461 gcgcgcgcta ccgcaacgcc ttcggcagca gcctctggta agccggctgg tcggcaagca
     2521 ggccctcgcg acggtgcgag aaatgcgggc cagtgcccca cccgcggccg ccagccctct
     2581 ggttcagggc tggcgcctgc tgcggctgat cgtgcatctg gtgcgcggac tggcgaccga
     2641 ggctttggtg ttcccgttcg tcggcagcga acgcgaattc gccatcatcc gccgctggtc
     2701 gcgcgggctg ttgcgcgtgc tcaacgtgaa gctgcgcgtg tcgggcactc cgcccggagg
     2761 ccgcacgccc acgttgctgg tgaccaatca catatcctgg ctcgatatct ggctgatcca
     2821 cgccgtgtgc ccggtgcgct tcgtcgccaa atcggatgtg cggcgctggc cggtgctggg
     2881 ctggctggtg gcacgctccg gcaccatctt catcgagcgc acgcggcgcc acgacaccgg
     2941 gcgcaccaac cgccgcatcg tcgaggtgct gtcccggggc gagcgcgtgg gcgtgttccc
     3001 ggagggcacc accaccgacg gaacgcatct gcgcccgttc cacgcctcgc tgttccagcc
     3061 cgccctcggg gcgggcgcgc gcatcgtgaa cgccgcgatc cgctatccgt tgcacgacgg
     3121 tgcgccgaac ctggacgtgg cctataccgg cgagcgctcg ctgctcgcct cgctgcgcct
     3181 gatcctggct cagcccaggc tgcacgcgga gctgatcttc tgcggcgtgc tcgagcccgt
     3241 cggcaagacg cggcgcgact tggcgcgcga gtcccaccgg ctcatcgcgc acgcgctggg
     3301 gctttcgcgt cccggcaccg aacctgaaac agccgacggt cctccagacg cagagccgtg
     3361 aggcgccgcc cccacaggca gccggtatcc agcccgaaca gatcggggcg cttgatcaac
     3421 cccagcgccg accagtggcc gaagatcagt ggcgtgtccc ggctgcggcg ctcgggcacg
     3481 tcgaaccacg gcagatagcc cgcgggcagc cgcttgggac ggcccttgtg cgcgaactcc
     3541 atctccccct cggcggtgca cagacgcatg cgcgtcatgg cgttcacgat cacgcgcagc
     3601 cggtcgtgac cggccagcgc gtcgctccag cgctgcggct ggttgccgta catgcgtgcc
     3661 aggaagctgc gataggtcgc cgcgcac
//
LOCUS       DASPGE010000052         5861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_717172, whole genome shotgun sequence.
ACCESSION   DASPGE010000052 DASPGE010000000
VERSION     DASPGE010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717172"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VD791_01670"
     CDS             complement(<1..971)
                     /locus_tag="VD791_01670"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV06709.1"
                     /translation="MEFNILQHFKGLEHQPMNPLNADEIGTSLFLTGNMGDRISAKMD
                     GYAKHAGLSRRGFLKSSLGFSAAMLAANEVTGMKFFEVGKAEAADIDAKKEAIQVARA
                     SADFIMDAHTHVCTRPQGYKKGVNTTERGMWFVDLLDGLGKAMGLPNGTSDMNIDNYG
                     KLLLEGSDTTVGVFNPFGFREDYGGEDMVPMAYQQQVRDRWPKSTVMLAGGLTPNQGL
                     PVTLDRLRQYVSQNKISGLKLYTFDSTRNKGWWFDDEKVAYPIWEECRKLGVKNIGCH
                     KGIPFGQFYARYAHVEDFDAAADDFLDLNFIAYHSAWPYHNELAALKG"
     gene            complement(1207..2133)
                     /locus_tag="VD791_01675"
     CDS             complement(1207..2133)
                     /locus_tag="VD791_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXV06710.1"
                     /translation="MAVPAPPVAAHGSRIGRFLPYAILAAGVLVVSTAAILIRLAQSE
                     GVPSLAIAAVRLGLAALVLLPLAWARTGRELAALSQRDLLLAAASGALLALHFWSWIA
                     SLEFTSVASSTVLVTTNPLWVGLASVVLLRERLPVWALVGIGLSFGGMILTFAADTAA
                     ATPSPNPALGNALALVGAVAASGYLIIGRALRARLGLLAYVALAYGAAAALLWIGVLW
                     TQTGMLGYSLLAWSCLVALALGPQLIGHTAFNWSLRHLSATFVALAILGEPVGSAILA
                     WVIFEERFTALQLAGFGLLLVGIYLGARGEHR"
     gene            complement(2185..2616)
                     /locus_tag="VD791_01680"
     CDS             complement(2185..2616)
                     /locus_tag="VD791_01680"
                     /inference="COORDINATES: protein motif:HMM:NF018391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HXV06711.1"
                     /translation="MIVYDLLCENAHRFEGWFASAEAFSHQHEAGHLSCPMCGSSSVR
                     KQLSAPYVQTAGGEPREHTVMARPGLMEEVRRKLIETVLQNTEDVGTRFPQEARAIHH
                     KEAPERAIRGLATGAQAKELREEGIDVFSLPAPAVPKDKLH"
     gene            2850..3839
                     /locus_tag="VD791_01685"
     CDS             2850..3839
                     /locus_tag="VD791_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HXV06712.1"
                     /translation="MSEDAHASRRSQHAAETAGWHLVERVPLARSHETAGEVIARLPG
                     HAYDYAGTVFLADEGRRLLGVIPLPTLLKAPPAKPVVELSESAHAVHAGMDQEHIASH
                     AIRHNMSAVPVTDAHGVLLGVVPPMVLLEVLRHEHVEDIHRLAGIKRETAHARTAIEA
                     PPVRRARDRLPWLVIGLAGSMVATFVVASFERALQSRVAIAFFIPGIVYLADAIGTQT
                     EAIAVRGLSLSRAPLAQLVLGELRTGLLIGSTLGLLSFPAVWLAYDMHLALAVSLSLL
                     AAGAVATTVGLLLPWLLAHTGRDPAFGSGPIATIIQDVLSLLIYFAIASLLLL"
     gene            3854..5107
                     /locus_tag="VD791_01690"
     CDS             3854..5107
                     /locus_tag="VD791_01690"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HXV06713.1"
                     /translation="MSKTPDDASLTRLRSMQRAATGLLLAAAAVYAVARVYESRHPAI
                     GYLRAFCEAAMVGGLADWFAVTALFRHPLGLPLPHTAILPSNKERIGEALGRFVERNF
                     LSTEVVAAKLAETDLAGTAARWLAQPERSAMLAGHAANLLPRLLDAVGDEPMRQFLHR
                     QVLAGLRRVEMAPLAADVLETLTAGNRHQELVDEVILQTRRLLSEAEPELRARVREKT
                     AWLWKKLGVDEAISDRLISAAEETLAEVAADPTHAWRQRFTDLVHEYVVRLRTSAEYA
                     KRAEALKQSLLDHPVLGGYVGSLWDEVRTRVREDAAREDSAIRASLHSALLRLADALL
                     AEPAVREAIDAGMRKALAEIVEARRHEVAELIAQTVRRWDAHTVSERIERAIGRDLQY
                     IRINGTVIGGLVGVVIHAVSNAIGG"
     gene            5211..5573
                     /locus_tag="VD791_01695"
     CDS             5211..5573
                     /locus_tag="VD791_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXV06714.1"
                     /translation="MPKSYTQITADINRGILKLREAQPDALTGFSAMAKGAMKSGALS
                     EPTKELIALAIGVAGHCEACIAFHAKSLARLNAPREQVMEALAVAVYMGGGPALMYAA
                     EAVHAFEEFSGGRSPDAA"
BASE COUNT          925 a         2051 c         1998 g          887 t
ORIGIN      
        1 cccttcagcg ccgccagctc gttgtgatac ggccacgccg agtggtaggc gatgaagttc
       61 aggtcgagga agtcgtccgc cgcggcgtcg aagtcctcca cgtgcgcata gcgcgcgtag
      121 aactgcccga aggggatgcc cttgtggcaa ccgatgttct tgacgcccag cttgcggcac
      181 tcttcccata tcgggtaggc gaccttctcg tcgtcgaacc accagccctt gttgcgcgtc
      241 gagtcgaagg tgtacagctt caggccggag atcttgttct gcgatacgta ctggcgcagg
      301 cgatcgagcg tcaccggcag cccctggttc ggcgtcaggc cgccggccag catgacggtg
      361 gacttcggcc agcggtcgcg cacctgctgc tgataggcca tcggcaccat gtcttcgccg
      421 ccgtagtcct cgcggaagcc gaacggattg aacacaccga ccgtggtgtc gctaccctcg
      481 aggagcagct tgccgtagtt gtcgatgttc atgtcgctgg tgccgttggg caggcccatg
      541 gccttgccca ggccatccag caagtccacg aaccacatgc cgcgctcggt ggtgttcacg
      601 cccttcttgt agccttgcgg ccgggtacag acgtgggtgt gcgcgtccat gatgaaatcg
      661 gcgctggcgc gcgcaacctg gattgcttct ttcttggcgt cgatgtcggc ggcttcggct
      721 ttgccgacct cgaagaactt catgccggtg acttcgttgg cggccagcat cgccgccgag
      781 aagcccaggg acgacttcag gaaaccgcgc cgggacaggc cggcgtgctt cgcgtagccg
      841 tccatcttgg cggaaatccg gtcgcccatg ttcccggtga ggaacagcga cgtaccgatc
      901 tcatccgcat tcaaagggtt catcggctgg tgctcgagcc ccttgaagtg ctggagaatg
      961 ttgaactcca tagccctctc ctccgttgtt ggaatggtct cgactcccgg ccacccttgc
     1021 cgcaccgcag gccaggatga tccatcgcgt actgtagtcc cctcgccggc acccagcaag
     1081 ggcctgcttc cttccggtaa gtcgctcgcg cgacaggagg ttacggtgct ttgcagcatt
     1141 ggcagtcccg tgtgcagact gccagcgccc agactgccag cgccgagccg ccacagcgag
     1201 aaatcgctag cggtgctcgc cgcgcgcgcc caggtagatc cccaccagca gcaacccgaa
     1261 cccggcgagc tgcagcgcgg tgaagcgctc ctcgaagatc acccaggcga ggatcgcgga
     1321 gcccaccggc tcgcccagga tggcgagcgc aacgaaggtg gccgagagat ggcgcagcga
     1381 ccagttgaac gcggtgtggc cgatgagctg cggccccagc gccagcgcca ccaggcacga
     1441 ccaggcgagc agcgagtagc ccagcatccc ggtctgcgtc cacagcacgc cgatccagag
     1501 cagcgccgcc gcggcgccgt acgccagcgc cacgtaagcg agcaggccca agcgggcgcg
     1561 cagcgcccgg ccgatgatca gatagccgga cgccgccacc gcgccgacca gcgccagcgc
     1621 gtttcccagc gccgggttgg gcgaaggggt ggccgccgcg gtgtcggccg cgaaggtgag
     1681 gatcatcccg ccgaacgaca gaccgatgcc cacgagcgcc cagacgggca ggcgctcgcg
     1741 cagcagcacc accgaggcga gcccgaccca cagcggattg gtggttacca gcacggtgct
     1801 gctcgctacc gaagtgaact cgagcgaggc gatccacgac cagaagtgca gcgcgagcaa
     1861 cgcgccggat gccgccgcca gcagcaggtc gcgctgggac agcgcggcca gctcgcggcc
     1921 ggtgcgcgcc caggccagcg gcagcagcac cagtgcggcg agcccgagcc ggaccgcggc
     1981 gatggcgagc gacggtacgc cttccgattg cgcgagccgg atcaggatgg ctgcggtgga
     2041 gacgaccagg acgccggccg ccagaatcgc atagggaagg aagcgcccga ttcgggaccc
     2101 gtgagccgcg accggtggtg cggggacagc catgcgccgg gtcccgggcc ctcgatcacc
     2161 tgtggtgcat cgggacttac gctgtcaatg cagcttgtcc ttgggcactg ccggcgcagg
     2221 gagagagaac acgtcgatgc cttcctcgcg cagctccttg gcctgggcgc ccgtggccaa
     2281 cccgcggatg gcgcgctccg gcgcctcctt gtggtggatt gcgcgcgcct cctgcgggaa
     2341 gcgcgtgccg acatcttcgg tgttctgcag aaccgtctcg atgagcttcc tgcgcacctc
     2401 ttccatcagg ccgggacggg ccatcacggt gtgctcgcgc ggctccccgc ctgcggtctg
     2461 cacgtaggga gcggacagct gcttgcgcac gctggacgag ccgcacatcg ggcaggacaa
     2521 gtggccggcc tcgtgctggt gcgagaaggc ctcggcggac gcgaaccacc cctcgaagcg
     2581 gtgggcgttc tcgcagagca ggtcgtaaac gatcatctca cgggcttgcg gcgttggagt
     2641 gcgactcgca gtctagtggg gcggcgccca atccacaagt cgccccgtca aaccgaaaca
     2701 ctaccggcac accgccaccg ttgcggccga ggcacggcgc ccggggttcg agggacatgc
     2761 gcagcgcgcc ctcgcgggaa tgttacgctt gcgatgcagc cgccggatgc acgccggcgc
     2821 attcaacgat cgggacgcag ccatacgcca tgagcgaaga cgcacacgcg agccgtcgca
     2881 gccagcacgc cgccgaaacg gccggatggc acctggtcga gcgcgtgccg ctggcgcgca
     2941 gccacgagac cgccggcgag gtcatcgccc gcctgccggg tcacgcctac gactatgcag
     3001 gcacggtgtt cctcgccgac gagggacgcc gcctgctcgg cgtcatccct ttgccgacgt
     3061 tgctcaaggc gccaccggcc aagccggtcg tggaactgtc cgagagcgct cacgcggtgc
     3121 acgcgggcat ggaccaggag cacatcgcct cgcacgcgat tcgccacaac atgtcggcgg
     3181 tgccggtgac cgacgcgcac ggcgtgctgc tcggcgtggt gccccccatg gtcctgctcg
     3241 aggtgttgcg ccacgagcac gtcgaggaca tccaccgcct ggccggcatc aagcgcgaaa
     3301 ccgcgcacgc gcgcaccgcg atcgaggccc cgcccgtgcg ccgtgcccgc gaccgcctgc
     3361 cgtggctggt catcggcctg gcgggcagca tggtggccac cttcgtggtg gcctccttcg
     3421 aacgtgcgct gcaatcgcgc gtggcgatcg cgttcttcat tcccgggatc gtctatctgg
     3481 ccgacgcgat cggcacccag accgaggcca tcgccgtgcg cggactgtcg ctcagccgcg
     3541 caccgctcgc gcagttggtg ctcggcgagc tgcgcaccgg actgctcatc gggtcgacgc
     3601 tcggtctgct gagtttcccc gcggtctggc tggcgtacga catgcacttg gcgctcgccg
     3661 tgtcgctgtc gctgctcgcc gcgggggcgg tagcgaccac ggtgggcctg ctgctgccct
     3721 ggctgctggc ccacaccggc cgcgacccgg ccttcggcag cggcccgatc gccaccatca
     3781 tccaggatgt gctgtcgttg ttgatctact tcgccatcgc cagcctcctg ctgttgtagg
     3841 ctgcgccgcc cgcgtgagca aaacacccga tgacgcgtcg ctcacccgcc tgcgctcgat
     3901 gcagcgcgcg gccaccgggc tgttgctcgc cgccgcggcg gtgtatgccg tggcccgcgt
     3961 gtacgaatcg cgccaccccg ccatcggcta cctgcgcgcc ttctgcgagg cggcgatggt
     4021 gggcggcctg gccgactggt tcgccgtcac ggcgctgttc cgccacccgc tgggcctgcc
     4081 gctgccgcac acggcgatcc tccccagcaa caaggagcgt atcggcgagg cgctgggccg
     4141 gttcgtggag cgcaatttcc tgtccaccga agtggttgcc gccaagcttg cggagaccga
     4201 cctggcgggc accgcggcgc gctggctggc gcaaccggag cgctcggcga tgctcgcggg
     4261 ccacgccgca aacttgttgc cgcgcctgtt ggacgcagtc ggcgacgaac cgatgcgcca
     4321 gttcctgcac cggcaggtgc tggccggcct gcgccgcgtc gagatggcgc cgctcgccgc
     4381 cgacgtgctg gagaccctca ccgccgggaa ccggcaccag gagctggtgg acgaggtcat
     4441 cctgcagacc cgcaggctgc tcagcgaagc ggagcccgag ctgcgcgccc gcgtgcgcga
     4501 gaagaccgcg tggctgtgga agaagctggg cgtggacgag gccatctccg atcgcctgat
     4561 ctcggccgcg gaggaaacgc tggcggaggt ggcggcggac cccacgcatg cctggcggca
     4621 gcgcttcacc gatctggtgc acgagtacgt ggtgcggctg cgcacctcgg cggaatacgc
     4681 caagcgcgcc gaagcgctca agcagtcgtt gctcgaccac ccggtgctcg gcggctacgt
     4741 cggctcgctg tgggacgagg tgcgcacgcg cgtgcgcgag gacgcggcgc gcgaggattc
     4801 ggcgatccgc gccagcctgc actccgctct gctgcgactg gccgatgcgc tgctcgccga
     4861 gcccgcggtg cgcgaagcga tcgacgccgg catgcgcaag gcgctggccg agatcgtcga
     4921 agcccgccgc cacgaggtcg ccgagctgat cgcacagacc gtgcggcgct gggacgcgca
     4981 caccgtgagc gagcgcatcg agcgcgcgat cgggcgcgac ctgcagtaca tccgcatcaa
     5041 cggcaccgtg atcggcggtc tggtcggagt cgtcattcac gcggtgtcga acgcgatcgg
     5101 cggataggac gattcgcgcc ggcgccgcta ccgggcggcc tttgatagca gtcaacccgg
     5161 ctcccggcat caccgctacc attcccatcg aacgttctgg agacccttac atgccgaagt
     5221 cgtacacgca gatcaccgcc gacatcaacc gcggcatcct caagctgcgc gaagcgcagc
     5281 ccgatgcgtt gaccggcttc agcgccatgg ccaagggtgc gatgaagagc ggtgcgctgt
     5341 ccgagccgac caaggagctc atcgcgctcg ccatcggcgt ggcgggccat tgcgaagcct
     5401 gcatcgcctt ccacgccaag tcgctcgcgc gcctgaacgc gccgcgcgag caagtcatgg
     5461 aagcgctggc ggtggcggtg tacatgggcg gcgggccggc gctgatgtac gccgccgaag
     5521 cggtacacgc cttcgaggag ttcagcggcg gtcgcagccc ggatgcggcg tagcggacgc
     5581 gctttgtgtg gtgcccccgg aaacgcagag caacgtgctc cccgcctctc acgcggcggg
     5641 gtgccggcga agccggcggg gcggtgtggt gtgaacgtcg ctgaaccacc ccatcgcgct
     5701 gcgcgcgcca cccctcctcg gagaggaggg gaacctaccg aaacgcgccc aacaccctgc
     5761 tcaggaacgc ctcgtggtcc cacgtccccg gctcgcgcgt caggcccagg cgcagcacca
     5821 ccagcttgcg gctcgggatc acggtgagca gttgcgcctc g
//
LOCUS       DASPGE010000053         3208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_484716, whole genome shotgun sequence.
ACCESSION   DASPGE010000053 DASPGE010000000
VERSION     DASPGE010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484716"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1394)
                     /locus_tag="VD791_01700"
     CDS             complement(63..1394)
                     /locus_tag="VD791_01700"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXV06715.1"
                     /translation="MNHAASGSRHTRLVISIAIVLAVAFVVALVLALLGPAPPRTVVM
                     ATGPEGGSYHLLAERYRALLEREGVELKILTTDGAVDNVQRLRDARSGVSVAFVQGGV
                     TSEQESPELVSLGTVFYEPVWLFVRGATPALRPDVFQGRRVSIGPQGSGTRKLALELI
                     GAVGVSLDGVETLSLTASAVAEAMPRGDLDFAVLVAPWESKAVRDLLIAEGVTALSFP
                     RADAHVALRPYLHKLVLPRGVADLAKDRPPDDLALVAPKASLVVRRDLHPAIAYLLLD
                     VASQLHSQPGIFQKAGEFPAAEPIDLPLSADARQFYQSGRPFLQRYLPFWVAVFAARL
                     LVILIPVVGVVYPLARLAPAIYGWGMRRRIFTLYGELKFLETEIDARAGRRPMDDLRA
                     GLDKLEQRANHMRVPVTFAHMLYTLRLHIRLVREHLERRIGASVEKPNGKE"
     gene            complement(1420..2169)
                     /locus_tag="VD791_01705"
     CDS             complement(1420..2169)
                     /locus_tag="VD791_01705"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXV06716.1"
                     /translation="MRLKDKVALITGGAHGMGESMARVFAREGAKVVIGDVLEGEGGE
                     VAASIRAAGGDALFVRLDVSEEAQWAEAVRATVARHGRLDILINNAGISGAVPDRTSV
                     EYYDRLMAVNARGTFLGIMAAAPQMQKTGGGAIVNLSSISGFIGQEFVHMGYNAAKGA
                     IRLLTKSAAVQYAKAGIRVNSVHPGMLPPMRTSVTSADPATRERIIASVPMGRSGRPE
                     EVAYAVLFLASDEASYITGTELVVDGGFLAV"
     gene            complement(2213..>3208)
                     /locus_tag="VD791_01710"
     CDS             complement(2213..>3208)
                     /locus_tag="VD791_01710"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HXV06717.1"
                     /translation="PLIRLRRCSEQTGCEILGKAEFLNPGGSVKDRAALFIVQDAERS
                     GALRPGGVIVEGTAGNTGIGLALVGNARGYRTVIVIPDTQSQEKKDTLRLSGAMLYEV
                     PAVPYKNPNNYVHVAQRLAQALTKSEPNGAIWAQQFDNVANRQGHYATTGPEIWRQTD
                     GKVHGFICAAGTGGTLAGVAAALRERNPGIKIGLADPPGAALYSYYSRGELKAEGSSI
                     TEGIGQGRITKNLEGLTVDFPFQIPDTETVQAVFDLLQYEGLCLGSSSGINVAGAVRL
                     AKQLGPGHTIVTVLCDSGLRYQSRLFNPQFLRSKDLPVPEWLERKVPPLPAGVMT"
BASE COUNT          515 a         1117 c         1071 g          505 t
ORIGIN      
        1 cgcgagcggt actcgtcctt gagcggcgcc tgcagggcac gcagctgatc ggcgttcatc
       61 tctcactcct ttccattggg tttctcgacg gacgcgccga tgcgccgttc cagatgttcg
      121 cgcaccagcc tgatgtgcag gcgcaacgtg tacaacatgt gggcgaaggt gaccggaacg
      181 cgcatgtggt tggcgcgctg ctccagcttg tccaacccgg cgcgcaggtc atccatcggc
      241 ctgcggcccg cgcgcgcatc gatctccgtt tcgaggaact tgagctcgcc atagagcgtg
      301 aagatgcggc gacgcatccc ccagccgtat atcgccggcg ccaagcgtgc cagcggatac
      361 accacgccca ccacggggat caggatcacc agcagacgcg ccgcgaacac ggccacccag
      421 aacggcaggt agcgctgcaa gaacgggcgc cccgactggt agaactggcg cgcgtccgcg
      481 ctgagcggca gatcgatcgg ctcggcggcg gggaattccc cggccttctg aaaaattccc
      541 ggctgcgaat gcagctgcga ggcgacgtcc agcaacaggt aggcgatcgc cggatgcagg
      601 tcgcgccgca ccactaggct ggccttgggt gcgaccagcg ccagatcatc gggcggccgg
      661 tccttcgcga gatcagccac gccccggggc agtaccagct tgtggagata agggcgcaac
      721 gccacgtggg catcggcacg aggaaacgag agcgcggtca cgccctcggc gatgagcagg
      781 tcgcgcaccg ccttggactc ccacggggcc accaggacgg cgaagtccag gtcgccacgc
      841 ggcatcgcct cggccactgc cgatgcggta agcgacagcg tctcgactcc gtccagactc
      901 actccgaccg cgccgatcag ttcgagcgcc agcttgcggg taccgctgcc ctgcggaccg
      961 atggacacgc gtcgcccctg gaacacatcc ggtctgagcg caggcgttgc gccgcggacg
     1021 aacagccaca ccggctcata gaacacggtg ccgagcgaca ccagctcagg agactcctgc
     1081 tcgctggtga cgccgccttg gacgaaagcg acgctgactc ccgagcgtgc gtcgcgcagg
     1141 cgctggacgt tgtccacggc gccatcggtc gtcaggatct tcagctcgac gccttcgcgc
     1201 tccagcagcg cgcggtagcg ctcggcgagc aggtgatagg agccgccctc cgggccggtc
     1261 gccatgacga cggtgcgcgg cggtgccggg cccagcagcg ccaggaccag cgcgacgacg
     1321 aacgcaaccg ccagaacgat cgcgatcgag atgacgagcc gggtgtgccg gctcccgctc
     1381 gctgcgtggt tcatccgcga gctcgctggc ggaaaggcat cagaccgcca ggaagccccc
     1441 gtccaccacc agctcggtgc cggtgatgta cgaggcctcg tccgaggcga ggaacaacac
     1501 cgcgtaggcg acttcctccg gccggccgga tcggcccatg ggaacgctgg cgatgatcct
     1561 ctcgcgcgtg gccgggtcgg cactggtgac cgaggtgcgc atcggcggca gcatgccggg
     1621 atgcaccgag ttgacgcgaa tgccggcctt cgcgtactgc acggcggcgg acttggtgag
     1681 caggcgaatc gcgcccttgg ccgcgttgta ccccatgtgc acgaactcct ggccgatgaa
     1741 gccggagatc gacgacaggt tcacgatcgc gccgccgccc gttttctgca tctgcggcgc
     1801 cgccgccatg atgccgagaa acgtgccgcg ggcgttcacc gccatcaggc gatcgtagta
     1861 ctcgacgctg gtgcgatcgg gcaccgcgcc gctgatgccg gcgttgttga tcagaatgtc
     1921 gaggcggccg tgacgcgcca cggtggcgcg caccgcttcg gcccactgcg cctcctcgga
     1981 cacgtcgagg cgcacgaaca gggcatcgcc gccggcggct cggatcgaag ccgcgacctc
     2041 gccgccctcg ccctcgagca cgtcgccgat caccaccttg gcgccctcgc gtgcgaacac
     2101 gcgcgccatg ctctcgccca tgccgtgcgc accgccggtg atcaacgcca ccttgtcttt
     2161 cagtctcact cgcctctccc ctattgtctt ccgcagcgct ccatgcagcg gctcaagtca
     2221 tcacccccgc cggcaagggt ggcacttttc gctccagcca ttccggtacg ggcaggtcct
     2281 tcgagcgcag gaactgcggg ttgaacaacc gcgactgata gcgcagcccc gaatcgcaca
     2341 gcacggtgac gatggtgtgg cccggcccca gctgtttcgc aaggcgcacc gcgccggcca
     2401 cgttaatgcc gctggagctg cccaggcaca gcccttcgta ctgcagcaga tcgaaaaccg
     2461 cctgcaccgt ctcggtgtcc gggatctgga acgggaagtc gaccgtcaag ccctccaggt
     2521 ttttggtgat gcggccctgg ccgatgccct cggtgatgga cgagccttcc gccttcagct
     2581 cgccgcggct gtagtagctg tagagcgcgg cgccgggtgg atcggccagc ccgatcttga
     2641 tgccgggatt gcgctcgcgc agcgccgcgg ccacgccggc cagggtgcca ccggtgccag
     2701 cggcgcagat gaagccgtgc accttgccgt cggtctgccg ccagatctcc gggccggtgg
     2761 tcgcgtagtg accctggcga ttggccacgt tgtcgaattg ctgcgcccag atggcgccgt
     2821 tgggttcgct cttggtcagc gcttgcgcca ggcgctgcgc cacgtgtacg tagttgttcg
     2881 ggttcttgta gggcaccgcg ggcacttcgt agagcatcgc gccggacagg cgcagcgtat
     2941 ccttcttctc ctgggactgc gtgtcgggaa tgacgatgac ggtgcggtag ccgcgcgcgt
     3001 tgcccaccag ggccaggccg atgccggtgt tgccggcggt gccctcgacg atcacgccgc
     3061 cgggccgcag cgcgccgctg cgctccgcat cttgcacgat gaacagggcg gcgcggtcct
     3121 tcaccgaccc gccggggttg aggaactcgg ccttgcccag gatctcgcag ccggtctgct
     3181 cggagcagcg gcgcaggcgg atgagcgg
//
LOCUS       DASPGE010000054         2369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1655809, whole genome shotgun sequence.
ACCESSION   DASPGE010000054 DASPGE010000000
VERSION     DASPGE010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655809"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VD791_01715"
     CDS             complement(<1..562)
                     /locus_tag="VD791_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HXV06718.1"
                     /translation="MAEETRGRRVHERWAHLRFSVVGQLLAAPPPKGELKAALRALAE
                     RSWVHPSSGEPVRFGVSTIERWYYRALKERVDPVGSLRRKIRADAGQQFAISAAVRQA
                     LIAQYAAHKGWSVQLHYDNLVALAESHKELKPLPSYTTLRRFMRANGLAKRRRLSPRR
                     TEGAERAEARLVAREVRSYEAEHVNGL"
     gene            complement(652..1368)
                     /locus_tag="VD791_01720"
     CDS             complement(652..1368)
                     /locus_tag="VD791_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06719.1"
                     /translation="MSSFGRQHFLYFLPLPHGQGSFRPALAIVVNTLSLMGRSPLYRD
                     LLADASFHKRLRKFDEELGQAARAEGCLRCSGVLHSARFPRKPRGRPAGLGVEDERRI
                     SFCCAVDGCRKRRTPASLRFLGSKLYLAAVVVLVAVLRHGMTVPRIQRLSELAGIDRR
                     TIERWQVWWRKVVPATAFWRNARAAFMPPLDEARLPDALIERFTVMPEACFQHDAPDR
                     LIALLRFLAPLTGDARMRAF"
     gene            1286..1789
                     /locus_tag="VD791_01725"
     CDS             1286..1789
                     /locus_tag="VD791_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HXV06720.1"
                     /translation="MAKAGRNDPCPCGSGKKYKKCCLPKEDTAALEVRAKAQAEREAR
                     AAADRLRDRQIGAERLARWAADDSDADGDPLDAASNAVIDLIEAGKLEEAEAAARDLL
                     VRYPDVHDGWDRLGMVYEARSNSREAADCYRKVIDFLRQHPDYADPEMAEDYAKLVAK
                     LDPPPAG"
     gene            1856..>2369
                     /locus_tag="VD791_01730"
     CDS             1856..>2369
                     /locus_tag="VD791_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXV06721.1"
                     /translation="MARPPASDCSARVTHGGQQLAFWNAHQDERGFATMHINHVRSGT
                     PIAAILRSARTPKGTEVRTVVKHVTRRLKMHWPRTRLIWRGDSHYGRNEVMQWCEDNG
                     EAFIFGLSGNSVLVDIRYVATSLEGSPEHLYENVYCQRGQMENLIKLHKAQLASDRMS
                     CHSATANQVRL"
BASE COUNT          414 a          817 c          803 g          335 t
ORIGIN      
        1 acaggccatt gacgtgctcg gcctcgtagc ttctgacctc gcgcgcaacg agccgggcct
       61 cggcgcgctc ggctccctcc gtgcggcgcg gcgacaggcg ccggcgcttg gcgagcccgt
      121 tggcgcgcat gaagcggcgc agcgtcgtgt aggacggcag cggcttgagc tctttatggc
      181 tttcggccag agcgacgagg ttgtcgtaat gcagctgcac gctccagccc ttgtgtgcgg
      241 catattgcgc gatgagagcc tgccgcacgg ccgcgctgat ggcgaactgc tggccggcgt
      301 cggcgcggat cttgcggcgc agtgagccga ccggatcgac acgctccttc agcgcccggt
      361 agtaccagcg ctcgatggtg gagacgccga agcggaccgg ctcgccgctc gaaggatgca
      421 cccacgagcg ttctgccaat gcgcgcagcg ccgccttgag ctcgcccttc ggcggcggcg
      481 cggccaagag ctggccgacg acggagaagc gcaagtgcgc ccaccgttca tgaacccgtc
      541 gtcctcgcgt ctcctctgcc atatcggcgc tcctccgatc agggtgcgtc gagcgtaggc
      601 acggcgctgg agcgcccgcg acgggcatcc tctgcgggcc ggccgtcgcc gtcagaaagc
      661 gcgcatgcga gcgtctccgg tgaggggggc caggaagcgc agcagcgcga tcagccggtc
      721 cggcgcgtca tgctggaagc atgcctccgg catgacggtg aagcgctcga tgagcgcatc
      781 cggcagccgc gcttcgtcga ggggtggcat gaaggcggca cgggcgttcc tccagaatgc
      841 cgtcgcgggg acgaccttgc gccaccacac ctgccagcgt tcgatggtgc ggcgatcgat
      901 acccgcaagc tcggaaagcc tctgtatgcg tggcaccgtc atgccatgtc gcagcacggc
      961 gaccagcacg acgactgccg ccaggtagag cttggagccg aggaaccgca gcgaggccgg
     1021 agtgcggcgc ttgcggcagc cgtcaactgc gcagcagaag ctgatgcgcc gctcgtcctc
     1081 cacgccaagc ccggccggtc ggccgcgcgg cttgcgcgga aagcgcgccg aatgcagcac
     1141 gccgctgcag cgcaggcagc cctctgcccg cgcggcctgc ccgagctcct cgtcgaactt
     1201 gcgcaagcgc ttgtggaagc tggcatcggc cagcaggtct cggtacaacg ggctcctccc
     1261 catcaggctc aaggtgttga ccacaatggc caaagccgga cggaatgatc cctgcccgtg
     1321 cggaagcggc aagaagtaca agaagtgttg cctgccgaag gaggacacgg ccgcgctcga
     1381 ggtgcgcgcc aaagcgcagg ccgaacgtga ggcgcgggcg gccgccgatc ggctgcgcga
     1441 ccggcagatc ggagccgaga ggctcgcgag gtgggccgcc gatgacagcg acgccgatgg
     1501 cgacccgttg gatgccgcct ccaacgccgt gatcgacctc atcgaggccg gcaagctgga
     1561 ggaggccgaa gccgccgccc gcgaccttct cgttcgctac cccgacgtgc acgacggctg
     1621 ggatcgcctc ggcatggttt acgaggcgcg cagcaacagc cgcgaggccg ccgactgcta
     1681 ccgcaaggtg atcgacttcc tgcgccagca ccccgactac gccgaccccg agatggccga
     1741 ggactacgcc aagctggtcg ccaagctcga cccgccgccg gctggctgac gagcacactg
     1801 gcggatcgcg ctgtgtgatg gccgcgataa cgcgctcatc acgaaccgtg ctcagatcgc
     1861 acggccacca gcctcagatt gctcggcgag ggttacgcac ggcggccagc agctcgcgtt
     1921 ctggaatgcg caccaggacg agcgcggctt cgccaccatg cacatcaatc acgtcaggag
     1981 cggcacgccg atcgccgcga tcctgcgctc cgcccgcacg ccgaagggca cggaggtgcg
     2041 gaccgtcgtc aagcacgtca ctcggcgcct caagatgcat tggccccgaa cgcgcctgat
     2101 ctggcgcggc gacagccact atgggcgcaa cgaggtgatg caatggtgcg aggacaacgg
     2161 cgaagccttc atcttcggcc tgtccggcaa cagcgtgctg gtcgacatcc gctacgtcgc
     2221 cacctcgctc gaaggcagcc ccgagcacct ctacgagaac gtctattgcc agcgcggaca
     2281 gatggaaaac ctgatcaagc tgcacaaggc gcagttggcg tccgatcgca tgagctgcca
     2341 cagcgcaacc gccaaccagg tgcgtctcg
//
LOCUS       DASPGE010000055         4181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_133713, whole genome shotgun sequence.
ACCESSION   DASPGE010000055 DASPGE010000000
VERSION     DASPGE010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133713"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VD791_01735"
     CDS             complement(<1..236)
                     /locus_tag="VD791_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018025740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06722.1"
                     /translation="MELTAQRLNAATEALRAMLGFEHPADAVLRAFFREHRVLGQRDR
                     GPVAELSFTALRHLRLLETLAGGRQPRRLLLAAAA"
     gene            complement(280..981)
                     /locus_tag="VD791_01740"
     CDS             complement(280..981)
                     /locus_tag="VD791_01740"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HXV06723.1"
                     /translation="MKRSLLALLLAAQLPAGALAAVPARVEITYAVFLGSTKIGEGRD
                     VFQHDGRTYRVVSESKTAGVAAMFYRLNIRRESQGRVTRAGLRPEAFSEERNGQPKRS
                     AAFDWERKQATLVDGDESQTVPLPDNSWDSTSFGYNFAFVPPEASGLVAHLTDGRRLR
                     EYRYALLGREKIDTELGALETLHVKKVQGPQDKRAFEVWLAVERHHMPVRIRYTDRNG
                     KVFDSVVTRIVFPEK"
     gene            complement(978..1613)
                     /gene="purN"
                     /locus_tag="VD791_01745"
     CDS             complement(978..1613)
                     /gene="purN"
                     /locus_tag="VD791_01745"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HXV06724.1"
                     /translation="MKRLVILISGRGSNMQALLEAPVPAVTGAVISNDPAAGGLAIAI
                     ARGVATQIVDHRAHADREAFDRALAQAVDAAAPDLIVLAGFMRVLTPWFVRRYFGRLI
                     NIHPSLLPAFPGMHTHRRALEAGVRLHGCTVHFVTAQVDHGPIIVQAAVQVLDDDTED
                     SLAARVLAQEHKILPRAVRWFCEGRLALEGNRVRLSAPTRAAQAIVCPEEA"
     gene            complement(1625..2683)
                     /gene="purM"
                     /locus_tag="VD791_01750"
     CDS             complement(1625..2683)
                     /gene="purM"
                     /locus_tag="VD791_01750"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HXV06725.1"
                     /translation="MSDTHRRRNLPRGLTYRDAGVDIDAGDALVQNIKPLARRTLREG
                     VLAGIGGFGALFEVPRGYRNPVLVSGTDGVGTKLKLAFELNRHDTVGIDLVAMSVNDI
                     LTQGAEPLFFLDYYACGKLDVQIATAVVGGIARGCEMAGCALIGGETAEMPDMYPPGE
                     YDLAGFAVGVVEKDRIIDGARIAPGDALIGLASSGAHSNGYSLIRRILERVRPDLSSD
                     FHGRSLGETILAPTRIYVKPVLELMQKIDVKGLAHVTGGGLLENVPRMLDAGLAARLE
                     RSAWPRAPLFDWLQAQGGVADEEMHRAFNCGIGMVVAVADADAARALELLTGAGELAW
                     RIGRVHPRATGAAQTVVA"
     gene            2848..3525
                     /gene="hda"
                     /locus_tag="VD791_01755"
     CDS             2848..3525
                     /gene="hda"
                     /locus_tag="VD791_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HXV06726.1"
                     /translation="MQLVLDLAPPAAPTLDNFEPGDNAEVLSAVRAWLNGASPQASVY
                     VWGPAGSGKSHLLRAAIVDAAARGGHAHYLAAGASLPPEALRAEWLAIDDVHALDAET
                     LAALFALLDRAAAGALRLLLAGDNAPRGLPLRTDVRTRIGAALVLRIKPLTDEDKVQA
                     LRRHADGRGFELTPEGAQYLLRYGRRDLPSLLALLDAADRYSLQTRRAVSVPLLREVL
                     QLADRGA"
     gene            3749..>4181
                     /locus_tag="VD791_01760"
     CDS             3749..>4181
                     /locus_tag="VD791_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HXV06727.1"
                     /translation="MRLALFDLDNTLLAGDSDFEWAQFLIDQGVLDREVYEARNQHFF
                     EQYKAGALDIHEFLDFQLKPLSRHPRRQLEDWHRDFMRSRILPIITQKARDLVQSHVH
                     DARAIVTATNSFVTGPIAREFGIDHLVATEPEEAGGEFTGRV"
BASE COUNT          599 a         1517 c         1442 g          623 t
ORIGIN      
        1 gccgccgccg cgagcagcag gcggcgcggc tgacggccgc cggccagcgt ttccagcaag
       61 cgcaggtggc gcagggcggt gaacgacagc tcggccaccg gcccgcggtc gcgctggccc
      121 agcacccggt gctcgcggaa gaaggcgcgc agcaccgcgt cggccggatg ctcgaagccg
      181 agcatggcgc gcagggcctc ggtggcggcg ttcaggcgct gagcggtcag ttccacgcgc
      241 gcgtggcggg tctccagcgc ggggcgggcg gcccggccgc tacttctccg gaaagacgat
      301 ccgcgtgacc accgagtcga acaccttgcc gttccgatcg gtgtaacgga tgcgcaccgg
      361 catgtggtgc cgctccaccg ccagccacac ctcgaaggca cgcttgtcct gcgggccttg
      421 cactttcttg acgtgcaggg tctcgagcgc gcccagctcg gtgtcgatct tctcgcgccc
      481 cagcagcgcg tagcggtatt cgcgcagccg gcgcccgtcg gtgaggtgcg ccacgagccc
      541 gctcgcctcg ggcggcacga acgcgaagtt gtagccgaag ctggtgctgt cccagctgtt
      601 atccggcagc ggcacggtct ggctctcgtc gccgtccacc agggtggcct gcttgcgctc
      661 ccaatcgaag gcggcgctgc gcttcggttg cccattgcgc tcttcgctga acgcctccgg
      721 ccgcaggccg gcgcgcgtga cgcggccctg gctctcgcgc cggatgttga ggcggtagaa
      781 catggccgcc acgccggcgg tcttcgattc gctcaccacg cgatacgtgc gcccgtcgtg
      841 ctggaacacg tcgcgcccct cgccgatctt cgtcgagccc aggaacacgg catacgtgat
      901 ctccacccgc gccggcaccg ccgccaatgc gccggccggt agctgggcgg cgagcaacag
      961 tgcgaggagc gaccgcttca tgcttcctcc ggacagacga tggcctgggc cgcgcgcgtg
     1021 ggggcgctga gtcgcacgcg attgccctcg agcgcgagcc ggccctcgca gaaccagcgc
     1081 accgcccgcg gcaggatctt gtgctcctgg gcgagcaccc ttgccgcgag gctgtcttcc
     1141 gtgtcgtcgt ccagcacctg caccgcggct tgcacgatga tgggaccgtg gtccacctgg
     1201 gcggtgacga aatgtacggt gcagccgtgc aggcgcacgc cggcctccag cgcgcgccgg
     1261 tgcgtgtgca tgccgggaaa cgcgggcagc agcgaggggt ggatattgat cagccgcccg
     1321 aagtagcgcc gcacgaacca cggggtgagc acgcgcatga agccggccag cacgatcagg
     1381 tccggcgcgg ccgcgtccac cgcctgggcc agcgcccggt cgaaggcctc ccgatcggca
     1441 tgagcgcggt ggtccacgat ctgcgtggcc acgccgcggg cgatggcgat cgccagtccg
     1501 ccggccgcgg ggtcgttgct gatcacagcg ccggtgaccg ccggcacggg cgcctcgagc
     1561 aacgcctgca tgttgctgcc gcgcccggag atgaggatga ccagccgctt cacgcgcgcc
     1621 cgcctcatgc cacgacggtc tgcgccgcac ccgtcgcgcg cgggtgcacg cgtccgatgc
     1681 gccaggccaa ctcgccggcg ccggtgagca actcgagcgc gcgcgcggcg tccgcgtcgg
     1741 ccaccgctac caccatgccg atgccgcagt tgaacgctcg atgcatctcc tcatcggcca
     1801 caccgccttg cgcctgcagc cagtcgaaca ggggcgcccg cggccacgca ctgcgctcca
     1861 gacgcgcggc caggcccgcg tccagcatgc gcggcacgtt ctccagcaag ccgccgccgg
     1921 tgacgtgcgc cagtcccttc acgtcgatct tctgcatcag ctccagcacc ggtttcacat
     1981 agatgcgcgt cggcgccagg atggtctctc ctaacgagcg accgtggaaa tcggacgaca
     2041 ggtccggccg cacccgctcg agaatgcgcc ggatcagcga gtaaccgttg gagtgcgcgc
     2101 cgctggaagc cagcccgatc agcgcatcgc cgggtgcgat gcgggcgccg tcgatgatac
     2161 gatctttctc caccacgccc acggcgaaac cggcgagatc gtactcgccc ggcggataca
     2221 tgtccggcat ctcggcggtc tcgccgccga tcagggcgca gccggccatc tcgcagccgc
     2281 gcgcgatgcc cccgaccacg gccgtggcga tctgcacgtc gagcttgccg caggcgtagt
     2341 agtccaggaa gaacaacggc tcggcgccct gcgtgaggat gtcgttgacg ctcatcgcca
     2401 ccagatcgat gcccaccgtg tcgtgacggt tcaattcgaa agcgagcttg agcttggtgc
     2461 ccaccccgtc ggtgccggac accagcaccg ggttgcggta gcccctgggc acctcgaaca
     2521 aagcgccaaa gccgccgatg ccggcgagaa caccttcccg cagcgtgcgc cgtgccagcg
     2581 gcttgatgtt ctgcaccagc gcgtcgccgg cgtcgatgtc cacgcccgcg tcgcggtagg
     2641 tcaggccccg gggtaggttc cggcgtcggt gggtgtcgct catggcgtgt ggcgcggacg
     2701 ctgcggcgga tcgggccggg gaaactgcgc taagatggcg cggttggcgt tcgccgtctc
     2761 ggcgaggcgg cattctactg caaaccgctt cccatgcgtt tccctcgggc gttgcctgcg
     2821 ccgcctgccc tgaccatcgc tccgccgatg cagctcgtgc tagatctcgc tccgcccgcg
     2881 gcacccacgc tcgacaactt cgagcccggc gacaacgccg aagtcctgtc cgcagtgcga
     2941 gcctggctga acggcgcgtc gccgcaggcg agcgtttacg tgtgggggcc agccggatcc
     3001 ggcaagagcc atctgctgcg cgcggcgatc gtcgacgcgg cggcgcgcgg aggtcacgcg
     3061 cactacctgg ccgccggggc gtcgctgccg ccggaggcgc tgcgcgcaga gtggctggca
     3121 atcgacgacg tgcacgcgct ggacgccgag accctggctg cgctgttcgc gctgctcgat
     3181 cgcgccgcgg cgggagcgct gcgcctgctg ctcgcgggtg acaacgcgcc gcgcggcctg
     3241 ccgttgcgca ccgacgtgcg cacccgtatc ggcgccgcgc tggtgctgcg gatcaagccg
     3301 ctcaccgacg aggacaaggt gcaggcgctg cgccgtcatg ccgacggccg cggcttcgag
     3361 ctcacgcccg aaggcgcgca gtacctgctg cgctacggtc ggcgcgacct gccatcgctg
     3421 ctggcgctgc tcgatgccgc cgaccgctac tcgctgcaga cgcgccgcgc ggtgtccgtg
     3481 ccgttgctgc gcgaggtgct gcagctcgcg gaccgcggcg cctagagcgg cgcgagcgcc
     3541 gcccgggcgc gggcgcggcc atgccgccgg caagcggttg tcggggagat gcagtcgcga
     3601 attcggctat actcgatcgc tacgccgcat ggcacgccca cccgttttga tctccttcgc
     3661 ccccacgcgt ttcctctggc tccagcggcg gctccggcgg cgctgcggct gtcgggcgat
     3721 ggcaacatag ccgcggcgga tgacggccat gcggctcgcc ctgttcgacc tcgacaatac
     3781 gctgctcgcc ggtgacagcg acttcgagtg ggcgcagttc ctgatcgatc agggcgtgct
     3841 cgaccgcgaa gtgtacgagg cgcgcaacca gcacttcttc gagcagtaca aggccggcgc
     3901 gctggacatc cacgagttcc tcgacttcca gttgaagccg ctgtcgcgcc atccgcgccg
     3961 gcagctcgag gactggcacc gcgacttcat gcgcagccgc atcctgccga tcatcacgca
     4021 gaaagcgcgc gacctggtgc agagccacgt gcacgatgcg cgcgccatcg tcaccgccac
     4081 caacagcttc gtcaccgggc cgatcgcgcg cgagttcggc atcgatcacc tggtggcgac
     4141 cgagccggag gaggcgggcg gcgagttcac cggacgcgtg c
//
LOCUS       DASPGE010000056         4864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_252153, whole genome shotgun sequence.
ACCESSION   DASPGE010000056 DASPGE010000000
VERSION     DASPGE010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252153"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1304)
                     /locus_tag="VD791_01765"
     CDS             complement(18..1304)
                     /locus_tag="VD791_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXV06728.1"
                     /translation="MIFPLLLVSAAACADDASRARIDALAAAVESKVIAWRRDIHAHP
                     ELGNREVRTAALVTAHLRALGLEVRTGVAHTGVVALLRGGKPGPVVALRADMDALPVT
                     EEVDLPFASKVRTQWAGRETGVMHACGHDTHVAMLLGAAEVLAGMKAQLPGSVKFIFQ
                     PAEEGPPPGEEGGAALMIKHGVLDNPRPDAIFGLHIGATYEVGQLAYRPGPVMAAADV
                     LRITVKGRQTHGSAPWGGVDPIVVAAQIVLGLQTIVSRQVNITKEPVVVTIGGIDGGN
                     RFNIIPDKVEMVGTVRSFDERLRDDVHHRIRLTAEGIAAASGASAEVAIEKPYAVTVN
                     DPALTERMRPTLARVVGEGNLLLAERRTGAEDFSFFAQQVPGLFVFLGGSPKGTDMSK
                     VAYNHSPRFYVDESALLLGVRTLLHLTIDYLSGAGK"
     gene            1460..1651
                     /locus_tag="VD791_01770"
     CDS             1460..1651
                     /locus_tag="VD791_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06729.1"
                     /translation="MKFDAPHGSVFGGYGFWSYSAAPNSQPSAGNSGPSAGTSPERGS
                     LSLRWLLDHARRMLRRTSA"
     gene            complement(1724..3775)
                     /locus_tag="VD791_01775"
     CDS             complement(1724..3775)
                     /locus_tag="VD791_01775"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HXV06730.1"
                     /translation="MSFFNLYRHNFVRVAVAIPEVRVADPAFNASQSVDLVARAASER
                     AVLVVLPELVLSAYSCEDLFHQQALLDACRDALAKVLEGTRELPVAAVLGLPLQVDDL
                     LFNCAVVVARGRILGVVPKTYLPSYREFYELRQFTPGDFSAREAIRLLGQEAPFGTRL
                     IFRVEEQPLLRFAVEICEDLWTPLPPSSSAALAGATVLVNLSASNVTVGKDDWRRQLV
                     ASQSGRCIAAYLYCAAGYGESTTDLAWDGHGMIYENGTRVAETERFAYRSQLVTADID
                     LDRLAQDRMRTNTFGQSMQRHREAVERFRSVPISLPLPADRTLPLARAYDRFPYVPAD
                     SATRDERCHEVYEIQVQGLVKRLRAMNAQHAVIGISGGLDSTQALIVCARAMDVMGLP
                     RANILAYTMPGFATSERTLDQSRRLMQAVGCSAHEIDIRPSCMQMFKDIGHPFAAGKP
                     VYDVTFENVQAGERTSHLFRLANLHGAPVVGTSDLSELALGWSTYGVGDHMAHYHVNA
                     SVPKTLIQHLIRYVARTGQLGADAGRVLPDVLDTTISPELVPAAGHSEPGQSTEAVIG
                     PYELQDFHLYYLLRFGYLPSKVAFMAWCAWRDRAAGVWPDVPVQHRRQYTIADVRKWL
                     GVFVRRFFQLSQYKRSALPNGPKVGSGGSLSPRSDWRAPSDSEAAAWLADVERIPARG
                     P"
     gene            complement(3795..4364)
                     /locus_tag="VD791_01780"
     CDS             complement(3795..4364)
                     /locus_tag="VD791_01780"
                     /inference="COORDINATES: protein motif:HMM:NF022586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HXV06731.1"
                     /translation="MPLTKPVSRKLMHTRTVHCAGYQRDDGLWDIEAHLTDTKPFDIP
                     SEDRGGTIPAGEPLHEMWIRLTVDLDLVVHDVEARTDHGPHHICANIVDNFKVLKGLV
                     IKSGWTQKTRELLGGTRGCTHLVELLGPVATTAFQTIYPARVKRDRDKPLTHKPGLVD
                     SCHAYASDGPIVKRRWPQFYTGPGGARDA"
     gene            complement(4399..>4864)
                     /locus_tag="VD791_01785"
     CDS             complement(4399..>4864)
                     /locus_tag="VD791_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06732.1"
                     /translation="YFGGQPPEELVSGTAFPEAHWGYVDKTSEIAPGVYAIALVSDTP
                     GTREMRELSLAIRTAAGLVLVVGCSHPGIERIVEAASAIDPRVRIVFGGFHMPAASDA
                     EISRVAGLLHDRWKVEQLAPGHCTGEPTFAHFKKVWGTAYRHAGVGSVIELP"
BASE COUNT          784 a         1694 c         1648 g          738 t
ORIGIN      
        1 aggcggaagc ggggccccta cttgccggcg ccgctgagat agtcgatcgt gaggtgcagc
       61 agcgtgcgca cgcccagcag cagcgccgac tcgtccacgt agaagcgcgg agagtggttg
      121 taagccacct tcgacatgtc cgtgcctttg ggcgaaccgc ccaggaatac gaacaggccg
      181 ggcacctgct gcgcgaagaa cgagaagtcc tccgccccgg tgcggcgttc ggccaggagc
      241 aggttcccct caccgaccac gcgcgcgagc gtcgggcgca tgcgctcggt gagcgcgggg
      301 tcgttgaccg tcaccgcgta cggcttctcg atcgcgacct ccgccgaggc gccggaggcc
      361 gccgcaatgc cctccgccgt cagccggatg cggtgatgga cgtcgtcgcg caagcgctcg
      421 tcgaagctgc gcacggtacc caccatctcg accttgtcgg gaatgatgtt gaagcggttg
      481 ccgccgtcga tgccgccgat ggtgaccacc accggttcct tggtgatgtt cacctggcgg
      541 ctgacgatgg tctgcaggcc gagcacgatc tgggcggcga ccacgatcgg atccaccccg
      601 ccccagggcg ccgagccgtg cgtctgccgg cccttcacgg tgatgcgcag aacgtcggca
      661 gccgccatca ccggacccgg gcgatacgcg agctgcccga cctcgtaggt ggcgccgatg
      721 tgcagaccga agatggcgtc gggtctcgga ttgtcgagca caccgtgctt gatcatgagc
      781 gcggccccgc cttcctcgcc cggcggcgga ccctcttccg ccggctggaa gatgaacttg
      841 acgctgccgg gcagctgcgc cttcatgccg gcgaggacct ccgcggcgcc cagcagcatc
      901 gccacgtgcg tgtcgtggcc gcaggcgtgc atcacgccgg tctccctgcc ggcccactgc
      961 gtgcgaacct tcgaggcgaa aggcaggtcc acttcttcgg tcaccggcag cgcgtccatg
     1021 tcggcgcgca gcgccaccac cggccccggc ttgccgcctc gcagcagcgc caccaccccg
     1081 gtgtgggcga ctccggtgcg cacctccagg cccaaggcgc gcaaatgcgc ggtgaccagc
     1141 gcggccgtgc gcacctcgcg gttgcccagc tcgggatgcg cgtggatgtc gcgccgccag
     1201 gcgatcacct tcgattcgac cgccgccgcc agcgcgtcga tgcgcgcgcg cgacgcgtcg
     1261 tcggcgcacg ccgccgccga caccagcagc aggggaaaaa tcacaaaccg tttcaatttt
     1321 ctctcgctct ttgtgtaatc acgcacagca tgacatgcgc cggcgcacca tgaggacgac
     1381 accaaagcgt tggcacaagt gatgctggag agcatgccac agcactgtca catgcgcagc
     1441 cgctgaggag acaagacaga tgaagttcga tgcaccgcat ggcagcgtgt tcggaggata
     1501 cgggttctgg agctactcgg ccgcgcccaa ctcgcagccg tccgccggca attccggtcc
     1561 atccgcaggg acgtcgccgg agcgcggctc gctgagccta cgctggttgc tcgatcacgc
     1621 ccggcgcatg ctgcgccgca cgagcgcctg atcgcgtcgc ttcccgccgc ggtgccgccg
     1681 aagtccggcg acaggcgccc gaaacacatt ccgcatcaca ctgtcagggc ccgcgggcgg
     1741 ggatgcgctc cacgtccgcc agccaggcgg cggcctcgct gtcgctcggc gcgcgccagt
     1801 cgctgcgcgg cgatagggag ccgcccgagc ccaccttggg gccgttcggc agcgcgctgc
     1861 gcttgtactg cgacagctgg aagaagcgcc ggacgaacac gcccagccat ttgcggacgt
     1921 cggcgatggt gtactggcgc cggtgctgca ccggaacatc gggccacacg cccgccgcgc
     1981 ggtcgcgcca ggcgcaccac gccatgaacg cgaccttgct cggcagatag ccgaagcgca
     2041 gcaggtagta gaggtggaag tcctgcagtt cgtagggacc gatcaccgcc tcggtgctct
     2101 gacccggttc actgtggccg gccgcgggca ccagctccgg gctgatggtg gtgtccagca
     2161 cgtccggcaa cacgcggccg gcgtccgcac cgagctgacc ggtgcgcgcc acgtagcgga
     2221 tcagatgctg gatcagggtc ttcggcacgc tggcgttcac gtggtagtgc gccatgtgat
     2281 cgcccacgcc ataggtggac cagcccagcg ccagctccga cagatcgctg gtgcccacca
     2341 ccggcgcgcc gtgcaggttg gccaggcgga acagatgact ggtgcgctcg cccgcctgca
     2401 cgttctcgaa ggtgacgtcg tacaccggct tgccggcggc gaagggatgg ccgatgtcct
     2461 tgaacatctg catgcaactg ggccggatat cgatctcatg cgcgctgcag cccaccgcct
     2521 gcatcaaccg ccgcgactgg tccagcgtgc gctcgctagt ggcgaatccc ggcatggtgt
     2581 aggcgaggat gttggcgcgc ggcagcccca tgacgtccat ggcgcgcgcg catacgatca
     2641 acgcctgggt cgagtccagc ccgcccgaga tgccgatcac cgcgtgctgc gcgttcatcg
     2701 cgcgcagccg cttgaccagc ccctgcacct ggatctcgta gacctcgtga cagcgctcgt
     2761 cgcgggtggc ggaatcggcc gggacgtagg ggaagcggtc gtaggcgcgc gccagcggca
     2821 gggtgcggtc ggccggcagc ggcagggaga tcgggacgct gcggaagcgc tccacggctt
     2881 cccgatgccg ctgcatcgac tggccgaagg tgttggtgcg catgcggtcc tgcgccaggc
     2941 gatcgaggtc gatgtccgcg gtgaccagct gcgagcggta ggcgaagcgc tcggtctcgg
     3001 ccacgcgcgt gccgttctcg tagatcatgc cgtggccgtc ccacgccaga tcggtggtcg
     3061 actcgccgta accggccgcg cagtatagat acgcggcgat acagcgtccg gactggctgg
     3121 cgaccagttg ccgccgccag tcgtccttgc ccaccgtgac gttggaggca gacagattca
     3181 ccagcacggt ggcgcccgcc agcgccgccg aggacgacgg cggcaacggc gtccacaggt
     3241 cctcgcagat ctccaccgcg aagcgcagca gcggctgttc ctccacgcgg aagatcaggc
     3301 gcgtcccgaa gggcgcctct tgtcccagca agcggatcgc ctcgcgcgcg ctgaagtcgc
     3361 ctggcgtgaa ctggcgcagc tcgtagaact cgcggtaact cggcagatag gtcttgggca
     3421 ccacacccag gatgcgcccg cgcgcgacca ccacggcgca gttgaacagc aggtcgtcca
     3481 cctgcagcgg caggcccagc acggccgcca ccggcagctc gcgcgtaccc tcgagcacct
     3541 tggcgagcgc atcccggcag gcatcgagca gcgcctgctg gtgaaacagg tcttcgcagg
     3601 agtaggccga gagcaccagt tccggcagca cgaccaggac cgcccgctcc gaggccgccc
     3661 gggcgaccag gtcgacgctc tgcgacgcgt tgaacgccgg gtccgccacc cgcacctcgg
     3721 ggatggccac ggcgacgcgc acgaaattat gccggtacag gttgaaaaag ctcacgggat
     3781 caatagtaca ggcactaggc gtcgcgcgcg ccgccgggtc cggtgtagaa ctgcggccag
     3841 cgccgcttga cgatcggccc gtcggaggcg tacgcatggc aggaatcgac cagcccgggc
     3901 ttgtgcgtga gcggcttgtc ccggtcgcgc ttcacgcgcg ccggatagat ggtctggaac
     3961 gcggtcgtcg ccaccgggcc gagcagctcc accagatgcg tgcaaccgcg agtgccgccc
     4021 agcagctcgc gggtcttctg cgtccagccg ctcttgatca ccagtccctt gagcaccttg
     4081 aagttgtcca cgatgttcgc gcagatgtgg tgggggccgt ggtcggtgcg cgcctcgacg
     4141 tcgtgcacta ccaggtccag gtccaccgtc aggcggatcc acatctcgtg cagcggctcg
     4201 cccgcgggaa tcgtcccgcc gcggtcctcg ctcgggatgt cgaacggctt ggtgtcggtg
     4261 aggtgggcct cgatatccca caagccgtcg tcgcgctgat aaccggcgca gtgcaccgtg
     4321 cgcgtatgca tcagcttgcg gctgacgggt ttggtcaatg gcattggcag caaccgtcgg
     4381 ttggaaaaag tgacatcgtt acggcagctc gatcacgctg ccgacgccgg cgtggcgata
     4441 ggccgtgccc cataccttct tgaaatgcgc gaaggtgggc tctccggtgc agtgaccggg
     4501 cgcgagctgc tcgaccttcc agcgatcgtg cagcaggccg gccacgcgcg agatctccgc
     4561 gtccgatgcc gccggcatgt ggaagccgcc aaacacgatg cgcacgcggg gatcgatcgc
     4621 gctggccgcc tcgacgatgc gctcgatgcc cgggtgcgag cacccgacca ccagcaccag
     4681 gcccgcggcg gtgcggatcg ccagcgacag ttcgcgcatc tcgcgagtgc cgggcgtgtc
     4741 cgacaccagc gcgatggcgt acaccccggg cgcgatctcg ctggtcttgt cgacgtaacc
     4801 ccagtgggcc tccgggaacg cggtgcccga caccagctcc tcgggcggct gcccgccgaa
     4861 gtat
//
LOCUS       DASPGE010000057         3975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_134963, whole genome shotgun sequence.
ACCESSION   DASPGE010000057 DASPGE010000000
VERSION     DASPGE010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134963"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..3517)
                     /locus_tag="VD791_01790"
     CDS             complement(5..3517)
                     /locus_tag="VD791_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXV06733.1"
                     /translation="MIVVDASTSAPTEARMTLRTVSLDDKYTLQSGRIFITGTQALVR
                     LPLMQHLRDRAERFHTGGYVSGYRGSPLGGYDQQLWAAKKHLEAHHIVFNPGVNEDLA
                     ATACWGTQQLGFHGASDYAGVFAIWYGKGPGVDRTGDVFRHGNLAGSHPRGGVLVLMG
                     DDHTCESSTTAHQSEYAMVDAMIPVLNPAGVQELLDYGICGWALSRYAGVWVGLKCVK
                     DVIDASGTVDVGPERVRVVTPTDFRMPPGGLNIRWPDQFLEQELRLHRYKLEAARAFA
                     RANGLDRLILDAPHAKLGVITCGKSYLDVRQALQYLGIDEAEANRLGLRLYKVGMTFP
                     LEPQGAMAFAQGLERIIVVEEKRSLIEWQLKEALYGQPNAPRIIGKQDERGALLFPSN
                     GALDPNHIAVEIGRRILERISDAQLQQRVKEQEQLESRVVEKPSMDRTPYFCSGCPHS
                     TGTRLPEGSIALSGIGCHFLAQYMNRNTAGFTQMGGEGASWIGEAPFVKTRHVFQNVG
                     DGTYFHSGFLAVRAAIAAGVNVTFKILYNDAVAMTGGQHIDGNLAPPQITRELLAEGV
                     KQVAVVTDEPHKYAAGAFPPEVQVHHRDDYDKVQRAIREIAGTTAIIYDQTCASEKRR
                     RRKRNAFPDPAKRAFINDLVCEGCGDCGVQSNCVSIVPQETEFGRKRRIDQSSCNKDY
                     SCVKGFCPSFVTVHGGGLRKGKGAAAGADAANLPFAVLPEPALPAIDRPYGIMVTGVG
                     GTGVVTIGAILGMAAHLEDKGFSSLDMAGLAQKGGAVLAHLQIAETPQDIKTVRLGSG
                     GAQLLLGCDLVVSASEKAMDTTRAGVTHALVNTHQQMTGDFTRNPDFAFPAQSLKHTI
                     VKGVGEANAEFVEATRVATALMGDSIATNMFILGFAYQRGLVPVSAQAIERAIELNGA
                     AVRMNQAAFLWGRRMAVDPATVERMLAPRETTAPMCISESLDEMSQRRGEFLTAYQNA
                     TYAQKYAALVERVRKEEQLRARGLTGLAEAVARYYFKLLAYKDEYEVARLYTDPAFME
                     KVKAQFEGDYSLHFHLAPPLLAKRNDKGELVKREYGAWMFGAFKLLAKLRFLRGTALD
                     VFGYSEERRTERKLIADYEKTVDQLLAQLTPDNHGLAVQIASIPEEIRGFGHVKAWHL
                     AAARQKEAKLLETWRAPTKARAAA"
     gene            complement(3653..>3975)
                     /locus_tag="VD791_01795"
     CDS             complement(3653..>3975)
                     /locus_tag="VD791_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXV06734.1"
                     /translation="DVVVEVGGAGTLEKSLAAVRVGGIVTLIGVLTGTAGPIPTAAIL
                     RRHLRVQGIYVGSREMFEEMNRAIARHRLRPRIDRSFAFEDARAAYDYLASKAHVGKV
                     VIKR"
BASE COUNT          585 a         1332 c         1366 g          692 t
ORIGIN      
        1 ccgcctacgc tgccgcccgc gccttcgttg gcgcgcgcca ggtctcgagc agcttggctt
       61 ccttctgcct ggccgcggcg aggtgccacg ccttcacgtg gccgaagccg cggatctcct
      121 cggggatcga cgcgatctgc acggcgagcc cgtggttgtc gggcgtgagc tgcgcaagca
      181 gctggtccac ggtcttctcg taatccgcga tcagtttccg ctcggtgcgc cgctcctcgc
      241 tatagccgaa cacgtcgagt gcggtaccgc gcaggaagcg cagcttcgcc agcagcttga
      301 acgcgccgaa catccaggcg ccatactcgc gcttcaccag ctcgcccttg tcgttgcgct
      361 tggcgagcag cggcggcgcc agatggaagt gcaagctgta gtcgccctcg aactgcgcct
      421 tgaccttctc catgaaggcc gggtcggtgt acaggcgcgc cacttcgtac tcgtctttgt
      481 acgccagcag cttgaagtag tagcgcgcca ccgcttcggc gaggccggtg agacctctgg
      541 ccctgagctg ctcctccttg cgcacgcgct cgaccagcgc ggcgtacttc tgcgcataag
      601 tggcgttctg gtaggcggtg aggaactcgc cgcggcgctg ggacatctcg tccagcgatt
      661 ccgagatgca catgggcgct gtcgtctccc tgggcgcgag catgcgctcc accgtcgccg
      721 gatccaccgc catgcgccgg ccccacagga aggccgcctg gttcatcctg accgccgcgc
      781 cgttcagttc gatggcgcgc tcgatggcct gcgccgacac cggcaccagc ccgcgttgat
      841 aggcgaagcc gagtatgaac atgttggtgg cgatggagtc gcccatcaga gcggtggcta
      901 cgcgcgtggc ctccacgaac tcggcgttgg cctcgcccac tcccttcacg atggtgtgct
      961 tgagcgactg ggcggggaag gcgaagtcgg ggttgcgtgt gaagtcgccg gtcatctgct
     1021 ggtgcgtgtt gaccagcgcg tgcgtgactc ccgcacgggt ggtgtccatc gccttctcgc
     1081 tggcggagac caccaggtcg cagcccagca gcagctgcgc gccgccggag cccaggcgca
     1141 cggtcttgat gtcctgcggt gtctcggcga tctgcagatg cgccagcact gcgccgccct
     1201 tctgcgccag gccggccatg tcgaggctcg agaacccctt gtcctccaga tgtgccgcca
     1261 ttccgaggat ggcgccgatg gtcaccacgc cggtgccgcc cacgccggtg accatgatgc
     1321 cgtagggccg gtcgatcgcc ggcagggccg gctcgggcag cacggcgaac ggcagattgg
     1381 ccgcgtcggc cccggcagca gcgcccttgc ccttgcgcaa gccgccgccg tgcaccgtca
     1441 cgaagctggg acagaagcct ttcacgcagg agtaatcctt gttgcaggag gactggtcga
     1501 tccggcgctt gcggccgaac tcggtctcct gcgggacgat cgacacgcag ttcgactgca
     1561 cgccgcaatc gccgcaaccc tcgcacacca ggtcgttgat gaaggcacgc ttggccggat
     1621 cggggaaggc gttgcgcttg cgccggcgtc gcttctcgga ggcgcaggtc tggtcgtaga
     1681 tgatcgcggt ggtgccggcg atctcgcgga tcgcgcgttg caccttgtcg tagtcgtcgc
     1741 ggtggtgcac ctgcacctcg ggcgggaagg ccccggcggc gtacttgtgc ggctcgtcgg
     1801 tgaccactgc cacctgcttg accccttctg cgagcagctc gcgcgtgatc tgtggcggcg
     1861 ccaggttgcc gtcgatgtgc tggccgccgg tcatggccac cgcgtcgttg tagaggatct
     1921 tgaaggtgac gttcacaccg gcagcgatgg cggctcgcac cgccaggaag cccgagtgga
     1981 agtaggtgcc gtcgccgacg ttctggaaga cgtgcctggt cttcacgaat ggcgcctcgc
     2041 cgatccagct cgcgccctcc ccgcccatct gggtgaaccc ggcggtgttg cggttcatgt
     2101 actgcgccag gaaatggcag ccgatgccgg agagcgcgat gctgccctcg ggcaacctgg
     2161 tcccggtgct gtgcggacag ccggagcaga agtacggcgt gcggtccatc gacggcttct
     2221 cgaccacacg cgactcgagc tgctcctgct ccttcacgcg ctgctggagc tgggcatccg
     2281 agatgcgctc gaggatgcga cggccgatct ccaccgcgat gtgattcggg tcgagcgccc
     2341 cgttgctcgg gaacagcagc gcaccgcgct cgtcctgctt gccgatgatc cgcggcgcgt
     2401 tgggctggcc gtagagcgct tccttgagct gccactcgat cagggagcgt ttctcctcga
     2461 ccacgataat tctctccagg ccttgtgcga atgccatcgc gccctgcggc tcgagcggga
     2521 aggtcatgcc caccttgtac aggcgcagac ccaggcggtt ggcctcggct tcatcgatgc
     2581 ccaggtactg cagcgcctgg cgcacgtcca ggtacgattt gccgcaggtg atcacgccga
     2641 gcttggcgtg cggcgcgtcc aggatcagcc ggtcgagccc attggcgcgc gcgaaggcgc
     2701 gcgcggcttc gagcttgtag cgatgaaggc gcagctcctg ttcgaggaac tgatcgggcc
     2761 agcggatgtt gagcccgccc ggaggcatcc ggaaatccgt gggcgttacc acgcgcacgc
     2821 gctccgggcc gacgtcgacg gtgccggagg cgtcgatcac gtccttcacg cacttcagcc
     2881 cgacccatac gccggcgtag cgcgacagcg cccacccgca gatgccgtag tcgagcaact
     2941 cctgcacgcc ggcggggttg agcaccggga tcatcgcgtc caccatcgcg tactcgctct
     3001 ggtgcgcggt ggtggaggac tcgcaggtgt ggtcgtcgcc catcagcacg agcacgccgc
     3061 ccctgggatg cgaacctgcg aggttgccgt ggcggaaaac gtccccggtg cgatccacgc
     3121 ccgggccttt gccgtaccag atggcgaaca cgccggcgta gtcgctcgcc ccgtgaaagc
     3181 cgagctgctg cgtgccccag caggcggtgg cggcaaggtc ctcgttcacc ccggggttga
     3241 agacgatgtg gtgcgcctcg aggtgcttct tcgcggccca cagctgctga tcgtagccgc
     3301 ccagcggcga gccgcggtag ccgctgacat agccgccggt gtggaatctc tccgcccggt
     3361 cgcgcaggtg ctgcatcagc ggcaggcgca ccagcgcctg ggtgccggtg atgaagatgc
     3421 ggccggattg cagcgtgtac ttgtcgtcca gggagacggt acggagggtc atgcgggcct
     3481 cggtcggggc gctcgtcgat gcgtcgacga cgatcatgtt gccacagaac cgttactgca
     3541 taaaccgcgg tccgccaagg gattctcccg gcgggagcgg attcgcaggc tcacttgcac
     3601 ctgccagcgt cgaacccgaa cggctgcggc ggcagcgcgg cgacgccgca gatcagcgct
     3661 tgatgacgac cttgccgacg tgggccttgc ttgcgaggta gtcgtaggcc gcgcgggcgt
     3721 cctcgaaggc gaagctgcgg tcgatgcggg gacgcagccg gtggcgggcg atggcgcggt
     3781 tcatctcctc gaacatctcg cgcgaaccga cgtagatgcc ctgcacgcgc aggtggcggc
     3841 gcaggatggc ggcggtggga atgggcccgg cggtgccggt cagcacgccg atgagggtga
     3901 cgatgccgcc gacgcgcaca gcggccagcg acttttccag ggtgccggcg ccgccgactt
     3961 cgaccaccac gtcgg
//
LOCUS       DASPGE010000058        13868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_604564, whole genome shotgun sequence.
ACCESSION   DASPGE010000058 DASPGE010000000
VERSION     DASPGE010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13868
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604564"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VD791_01800"
     CDS             complement(<1..211)
                     /locus_tag="VD791_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06735.1"
                     /translation="MGMSETVLAAMIGAGATLFTAFFSLLSSFRKPASDRRTTTGVGF
                     RSLMWILALVGAAGVGGFAYAEYRAQ"
     gene            complement(365..1084)
                     /gene="trmB"
                     /locus_tag="VD791_01805"
     CDS             complement(365..1084)
                     /gene="trmB"
                     /locus_tag="VD791_01805"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HXV06736.1"
                     /translation="MDRRDDKTTETRQRRPIRSYVLRQGRMSSAQQRAYDTLLHRFAL
                     PYAPAIADLVAAFGRTAPTVLEIGFGMGETTATIAQAHPEIDYLGVEVHAPGIGSLLK
                     RIDELGLTNVRIVQHDATEVVRHMLAPGTLDGVHVFFPDPWPKKRHHKRRLLQPAFVA
                     LLASRLKPGGLLHVATDWQDYAEQALAVLAAEPLLENTAPRFAPRPAHRPRTKFEQRG
                     LKLGHGVWDLLFRRVAAAGPA"
     gene            complement(1197..1997)
                     /locus_tag="VD791_01810"
     CDS             complement(1197..1997)
                     /locus_tag="VD791_01810"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HXV06737.1"
                     /translation="MDQLIIAGKRYASRLLVGTGKYKDFEQTRAAIEASGAQIVTVAI
                     RRTNIGQNPNEPSLLEAIPLSKYTILPNTAGCYTAEDAVRTLRLARELLDGHALVKLE
                     VLGDPHTLYPNVVETLAAARTLVKEGFEVMVYTSDDPIVAKQLEEIGCVAVMPLASLI
                     GSGMGILNPWNLQIVIDKAKVPVLVDAGVGTASDAAIAMELGCDGVLMNTAIAAAKDP
                     VRMAGAMRKAVEAGREAWLAGRMAKKPYSAAPSSPTSGLIAPVKGKAA"
     gene            complement(2079..2279)
                     /gene="thiS"
                     /locus_tag="VD791_01815"
     CDS             complement(2079..2279)
                     /gene="thiS"
                     /locus_tag="VD791_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HXV06738.1"
                     /translation="MALTINGERRDFEGPLTAAELIDRLQLGGKRVALERNGQIVPRG
                     AFPHERLGDGDRLEIVVAVGGG"
     gene            2349..2422
                     /locus_tag="VD791_01820"
     tRNA            2349..2422
                     /locus_tag="VD791_01820"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2381..2383,aa:Gly,seq:ccc)
     gene            2463..3806
                     /locus_tag="VD791_01825"
     CDS             2463..3806
                     /locus_tag="VD791_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HXV06739.1"
                     /translation="MPAQEVPMLQENVPDLARFVEPDRVRKEVYTDPAIFELEMERIH
                     ERVWIYCGHESQVRKPGDFYTVQIGRQPMLLVRGADGKVSVLYNRCPHRGSMMCGERH
                     GNTGEFFRCSYHAWTFHHDGTLRNIPMMESGYAGTALGRDNPDCNMKRAARVESYRGF
                     VFASLAPEGPSLAEFLGPARIAFDDMCDRAPQGEVEIVPNCFRVIQHSNWKIFLENQL
                     DALHPSVTHQSTGRAARDVELMIEKATGKAPLAYHMLSAFSTVTIDKWDNFQTLNYPY
                     GHCILTGYMGLRPQDPDTLEYERVLAKAYGEQRKEQILCTNIHHVLLYPGLSVQSPLQ
                     QLRAVRPLGVDRTLTEIWHFRLKGAPEPIYRRALAYYNLVNSPSTMINADDLENFWKV
                     QQGLASDGGDWVSFHRHHGRDLERGGVVQTGEGMGTSEAPMRNQMKAWAQYMARV"
     gene            3810..4430
                     /locus_tag="VD791_01830"
     CDS             3810..4430
                     /locus_tag="VD791_01830"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HXV06740.1"
                     /translation="MATPSRGKKKAPPKQAPGTSAKKMSMRKAAGRPSPKAARAQRRA
                     GAAADLHHEIEQFLYRQAELLDAKRWQDYIDLFADDGVYWMPASPQDRTWDGVPAIFA
                     EDKNLMTVRMKRVLHPDAWSQRPLWGTNHVVGNVILQAADGAPDELVVRSRFHMMELR
                     RDNVRHFAGAYTHHLKRTPDGLRIKLQRVDMTNAQAAYDYVLQVWV"
     gene            4443..7022
                     /locus_tag="VD791_01835"
     CDS             4443..7022
                     /locus_tag="VD791_01835"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXV06741.1"
                     /translation="MKALRYALYAVGALALLLIVAIGVLAATFDPNDYKPRIVQLVHD
                     KTGRTLSIAGDIGLKVFPKLGVQLGKLSLSERDGSGEFAHLEAAQVYVALLPLLSNRV
                     VVDEVRVDGLRANLIKFEDGSTNFDDLAKGGQTPSETPAPSETPAGRPIELDVDGIRV
                     TDSRLTWQDRTSGNDLAIHIAEFKTGRIADKRPGDIQLSAAVQGAKPRVDLRIGLECR
                     LTFDLEQQLYRLQNLSANLEGAALDFTDIAVKVGGDLEALAAQRRVALSDLRLEGKAA
                     RGTNRYNLDLSAPAIESTPEALAVQGLSLAATGSLGAMQLTQSSIKAPTVRVNLARNE
                     MLVEGLSLSARARQDGDNLDIGLSAPRLQVTPDSAGGESMVLTLKLDGAQRGADATVR
                     VSGLEGSARALKIQSLALELDARQQDNAIKGRLTTPVTGNLETRVFELPKLAGDFTVT
                     GPTLPGKRLQVPLSGLVRADLGKERVIGDIATRFDESNIKAKGGMTGFAAPAYDFDVA
                     IDALNLDKYRAPEKKETTAAQGPQRDEPIDLSALKGLNLKGEVRIGRLQASNVKAANL
                     RVSLRASNGKLDVDPLSANLYQGTVKGAIGVDANGNQFAVNQTLTGVAIGPLLRDAVQ
                     KDILEGKGSVALNVTTRGATVSELTRALDGTARTVLRDGAVKGIDLAGTVRRVKTKLT
                     GGDVEGSAAQGEKTDFSEMSASFTIRKGVARNEDLDLKSPFLRVTGSGEVDIPQSRLD
                     YVVKTSVVGTTAGQGGKELSELRGLTIPVRVAGPFDKLGYKVQFSQMVRGSTKEQLEA
                     AKRTGKEALEGAARGKLETLLGGPGGPEEAKEGQPGGEQAHQAAPKRPQDEVKDKLKE
                     LLR"
     gene            7019..8116
                     /gene="mutY"
                     /locus_tag="VD791_01840"
     CDS             7019..8116
                     /gene="mutY"
                     /locus_tag="VD791_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HXV06742.1"
                     /translation="MNAGAVGPSFAARLIAWQKTQGRHELPWQSTRDPYRIWLSEIML
                     QQTQVAAVVPYYERFLARLPDLAALAAAQEDAVLALWAGLGYYARARNLHRAAQTIVQ
                     RHRGIFPRELDAVRALPGVGRSTAAAICAFAYGTRAAILDGNVKRVLTRHFGIDGYPG
                     ERRIENALWRLSESLLPARDVEAYTQGLMDLGATLCVRGNPRCGACPLQDGCVARREG
                     RTQDWPTVRPAKALPQRRTTMLVLVSRGELLLEKRPASGIWGGLWCFPEIDGARFDRE
                     AGLDWESGLERECLARFGLHAGASRRLRDIEHGFTHFRLRITPVLVRTDRAPGAGEPG
                     SLWLTPADARGAAIPAPVRKIIDLIEVNRDS"
     gene            complement(8196..8783)
                     /locus_tag="VD791_01845"
     CDS             complement(8196..8783)
                     /locus_tag="VD791_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HXV06743.1"
                     /translation="MALEIPIFPLRTVLFPGGLLPLKIFEQRYLDMTKTCIRDASPFG
                     VCLLREGAEVGAPAVPYPIGCTALIRDWDMPHLGLFHLLCRGQSVFRIAEHWAVRSGL
                     LYGLVELQDASPSRPLAPSYRPLADLLAKIIDKVGADNFPSPVRLDDADWVACRLAEV
                     LPLEVELKQRMLELHDPQARLERLRTFLQSRSVAV"
     gene            complement(8799..9416)
                     /locus_tag="VD791_01850"
     CDS             complement(8799..9416)
                     /locus_tag="VD791_01850"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HXV06744.1"
                     /translation="MNGTVAVAFTGASGMPYGVRLLECLLASGARVYLLYSQASQIVA
                     RQEMDLALPGRAKEAETFFGARFRAQPGQLTAFGREEWFAPVASGSNPPDAMVICPCS
                     MGTLAAVAQGLADNLIERAADVMLKEARKLVLVPRETPLSAIHLENMLRLARAGAVVL
                     PANPGFYHHPRRVEDLVDFVVARVLDQLGIAHSLTPRWGEAAPQA"
     gene            complement(9413..9952)
                     /locus_tag="VD791_01855"
     CDS             complement(9413..9952)
                     /locus_tag="VD791_01855"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HXV06745.1"
                     /translation="MLALSLSAAAQAADWVRVGTPDQHQHFYDRSKLTIEKDEITYWR
                     RVLFRTPQPARSGKARMAMYRERVDCARHSYRTLGYLLYAQDGAILENVYTPEAPSQP
                     IIPETVGDRFETLMCLIVEQDQASRAQAEDALPASPEALRTEVERLEARLRELKEQLS
                     TAAPADAGSAPPPDTGEAQ"
     gene            complement(10080..10919)
                     /gene="rapZ"
                     /locus_tag="VD791_01860"
     CDS             complement(10080..10919)
                     /gene="rapZ"
                     /locus_tag="VD791_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HXV06746.1"
                     /translation="MRVILVTGMSGSGKSIALNVLEDIGYYCVDNLPANLLVEVVDFL
                     AQSGYARVGVSVDVRSGPTLKELPASVVELRRRQVDLRVLFLDAKTGTLVKRFSETRR
                     RHPLSDDARTLPECIDAERAMLQDIVALAHRIDTSELNTNALRAWVKDFAGLAGSGMT
                     LLFQSFGFKHGIPLDADLVFDVRCLPNPHYDPVLQPLTGNDDQVIAFMEADADAQKML
                     ADISAFLDAWLPCYLRDNRSYLTVAIGCTGGRHRSVYFSEVLARRFEPTLPVIVRHRE
                     LAQ"
     gene            complement(10972..11913)
                     /gene="hprK"
                     /locus_tag="VD791_01865"
     CDS             complement(10972..11913)
                     /gene="hprK"
                     /locus_tag="VD791_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HXV06747.1"
                     /translation="MPLVSVAQLFKDNQEALKLSWLGGETHAGNALDTRRIYQANEGL
                     IGHLNFYHPNWIQIFSPTEAAYFSAMGEADCEQALARLHASGLACVIVSDGERAPDTL
                     RRFAERMNAPLIGSPLPSLQIIWVIRTYMGRALAEFVTRHGVLLDVLGMGVLLTGDSG
                     VGKSELALELITRGGGLVADDVVELYHIAPETLEGRSPELLKDFLEVRGLGMLNIRTI
                     FGETAVRVRKNLKLIVQLERPVGGVIPGLERLPLTASFEEILGVKVRKVVLPVAAGRN
                     LAVLVEAAVRNYVLQLRGIDSTQEFIQRQEQHFGREP"
     gene            complement(11936..12415)
                     /locus_tag="VD791_01870"
     CDS             complement(11936..12415)
                     /locus_tag="VD791_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HXV06748.1"
                     /translation="MAPAISLLASLLPTSHVALDIAAQDKRAVFEHAATLLEGKTGIK
                     RGHILDSLLARERMGSTGLGQGIAIPHGRVKGLLAAVACFLRTRAPIAFDAPDDEPVR
                     LIFVLLVPEKSTEAHLEILSQLAEMFSDREMRAALAAAPDALAAQRLIAGWQARTAN"
     gene            complement(12456..12779)
                     /gene="raiA"
                     /locus_tag="VD791_01875"
     CDS             complement(12456..12779)
                     /gene="raiA"
                     /locus_tag="VD791_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HXV06749.1"
                     /translation="MNLQLTGHHLDITPAIRSYVTSKLERITRHFDHVIDVNVIMSVD
                     KLQQRIEATVHVRGRDIFCESIDADMYAAIDGLVDKLDRQILKHKEKAVEQRYNGANR
                     HRAGG"
     gene            complement(12806..>13868)
                     /gene="rpoN"
                     /locus_tag="VD791_01880"
     CDS             complement(12806..>13868)
                     /gene="rpoN"
                     /locus_tag="VD791_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HXV06750.1"
                     /translation="LLWQVNLLKCSDRDKRIVAMLVDTLDDDGYLTQDLDELAAMLPA
                     EAGIDREDLQIGLRHLQQLEPAGIGARDLSECLALQLEAMPGDTPYRAEALTTVRNHL
                     DLLASRDYARLKKLLKCSDEVLRGTQSLITSLNPRPGANFSGEEARYIVPDVVVKKVK
                     GLWRASLNPDAMPKLRINRMYADILHRNRDASLQQLAGQLQEAKWLIKNVQQRFDTIL
                     RVSQAIVDRQRHFFEHGEVAMRPLVLREIADTLGLHESTVSRVTTQKFMLTPRGIFEL
                     KYFFGSHVTTETGGACSATAIRALIKQLVAEEDSRKPLSDSQISEILGKQGIVVARRT
                     IAKYRESLQILPVNLRKSI"
BASE COUNT         2207 a         4700 c         4844 g         2117 t
ORIGIN      
        1 gttgcgcgcg gtactcggcg taggcgaaac cgccgactcc cgccgccccc accagcgcga
       61 ggatccacat cagcgagcgg aacccgacgc ccgtagtggt gcgccgatcg ctcgccggct
      121 ttcggaacga gctgagcagc gaaaaaaagg cggtgaacaa cgtggcgccg gcgccgatca
      181 tggcggccag gacggtctcg gacattccca ttgcactgct ccgcattcgg gcccggccgg
      241 ggccggaccg gccccgcggg ggggcggggg gatctgacga cgggttattc tgtccgccgg
      301 cgccggcccg ccctcgagtc gggccggaaa cggctggaac agcgaggaag aagcaagttc
      361 cgtgtcacgc cgggccggct gcggcaacgc ggcgaaacag cagatcccac acgccgtggc
      421 ccagcttcag gccgcgctgc tcgaatttgg tgcgcgggcg atgcgccggt cgcggggcga
      481 accggggcgc cgtgttctcg agcaggggct cggcggccag gacggcgagg gcctgctcgg
      541 cgtagtcctg ccagtcggtg gccacgtgca ggaggccgcc cggcttgagc cgcgaggcga
      601 gcagcgcgac gaaggccggc tgcagcaggc ggcgtttgtg gtggcgcttc ttgggccagg
      661 gatccgggaa gaacacgtgc accccgtcca gcgtccccgg ggcgagcatg tgccgcacca
      721 cctcggtggc gtcgtgctgg acgatgcgca cgttggtgag ccccagctcg tcgatccgct
      781 tcagcaggct gccgatcccg ggtgcgtgca cctccacgcc gagatagtcg atctcgggat
      841 gcgcctgggc gatggttgcc gtggtctctc ccatgccgaa gccgatctcg agcaccgtcg
      901 gggccgtgcg gccgaaggcg gcaacgaggt cggcgatggc cggagcgtag ggcagcgcaa
      961 agcgatggag cagcgtgtcg taggcgcgct gctgcgcgct cgacatccgt ccctggcgca
     1021 gcacgtagct gcggataggg cggcgctgcc gcgtttccgt ggtcttgtcg tcgcggcgat
     1081 ccaagggtta tgggtcggcc aagatgtcgc gggttggctg gaacggcagg ggaatccggg
     1141 cgtcccgcgc cgttcctggg acggtcccgc ggaccagtcc gtgccgcacc aggcgcttac
     1201 gccgccttgc ccttgaccgg cgcgatcaac cccgaggtcg gcgagctcgg cgcggccgag
     1261 tacggcttct tcgccatgcg cccggcgagc caggcctcgc gcccggcctc caccgcctta
     1321 cgcatcgcgc ccgccatgcg caccggatcc ttggccgccg cgatggccgt gttcatcagc
     1381 acgccgtcgc agcccagctc catggcgatg gcggcgtccg aggcggtgcc cacgcccgca
     1441 tccaccagca ccggcacctt ggccttgtcg atgacgatct gcaggttcca cgggttgagg
     1501 atgcccatgc cggagccgat caggctggcg agcggcatca ccgccacgca gccgatctcc
     1561 tcgagctgct tggcgacgat cgggtcgtcg gaggtgtaca ccatcacctc gaacccctcc
     1621 ttcaccagcg tgcgcgcggc ggccagcgtt tccacgacgt tcgggtacag cgtgtgcggg
     1681 tcgcccagca cctcgagctt gaccagggcg tggccatcca gcagttcgcg cgccaggcgc
     1741 agggtgcgca ccgcatcctc ggcggtgtag caaccggcgg tgttgggcag gatggtgtac
     1801 ttggacagcg ggatcgcctc cagcaggctc ggctcgttcg ggttctggcc gatgttggtg
     1861 cggcggatcg ccaccgtcac gatctgcgcg ccgctcgcct cgatggccgc gcgcgtctgc
     1921 tcgaaatcct tgtacttgcc ggttcccacc agcaggcggg aagcgtagcg cttgccggcg
     1981 atgatgagtt ggtccatagc gttgcaccac agttgggccg cgtccgcaga gtcacatctc
     2041 tcggtcacgc ttgccggtca cgtccttcgg tcacagtatc agccgccgcc caccgcgacc
     2101 acgatctcca gccggtctcc gtcgcccagg cgctcgtgcg ggaacgcgcc gcgcggcacg
     2161 atctgcccgt tgcgctccag cgcgacgcgc ttgccgccca gctgcaggcg gtcgatcagc
     2221 tcggccgcgg tcagcggccc ctcgaagtcg cggcgctcgc cgttgatggt gagagcgatc
     2281 acgaagcaaa ttttaccatg cgcgccgcgg ccaaagcgcc gctggaatcg agtacactac
     2341 tcctcgccgc gggcgtagtt caatggtaga acgaaagctt cccaagcttt agacgtgggt
     2401 tcgattccca tcgcccgctc cagcccgtca cgcagcgcct gaagtccaga ccacgcccgc
     2461 acatgcctgc gcaggaggtg cccatgctcc aggagaacgt ccccgacctc gcccgcttcg
     2521 tcgagccgga ccgcgtgcgc aaggaggtct acaccgatcc ggccatcttc gagctggaga
     2581 tggagcgcat ccacgagcgc gtgtggatct actgcggcca cgagagccag gtgcgcaagc
     2641 ccggcgactt ctacaccgtg cagatcggcc gccagcccat gctcctggtg cgcggcgcgg
     2701 acggcaaggt gagcgtgctc tacaaccgct gcccgcaccg cggctccatg atgtgcggcg
     2761 agcgccacgg caacaccggc gagtttttcc gctgctccta ccacgcctgg acgtttcacc
     2821 atgacggcac gctgcgcaac attccgatga tggaatcggg gtatgccggc accgcgctcg
     2881 gccgcgacaa ccccgactgc aacatgaagc gcgcggcgcg ggtggagtcc taccgcggct
     2941 tcgtgttcgc cagcctggcg ccggaggggc cgtcgctcgc cgagttcctg ggaccggcgc
     3001 gcatcgcctt cgacgacatg tgcgaccgcg cgccgcaggg cgaggtcgag atcgtgccga
     3061 actgcttccg cgtgatccag cattccaact ggaagatctt cctcgagaac cagctcgacg
     3121 cgctgcatcc gtcggtgacg caccagtcca ccggccgcgc cgcgcgcgac gtcgagctga
     3181 tgatcgagaa ggccaccggc aaggcgcccc tcgcctatca catgctgtcg gcgttctcca
     3241 ccgtcaccat cgacaagtgg gacaacttcc agacgctgaa ctatccctac ggccattgca
     3301 tcctgaccgg ctacatgggg ctgcgaccgc aggaccccga caccctcgag tacgagcgcg
     3361 tgctggcaaa ggcctacggc gagcagcgca aggagcagat cctgtgcacc aacatccacc
     3421 acgtcctgct ctatccggga ctgtcggtgc agtcgccgct gcagcagctg cgcgcggtgc
     3481 ggccgctggg cgtggaccgg acgctgaccg agatctggca cttccgcctg aagggtgcgc
     3541 ccgagccgat ctatcgccgg gcgctcgcct actacaacct ggtgaactcg ccgtccacca
     3601 tgatcaatgc cgacgacctg gaaaacttct ggaaggtgca gcagggcctg gcgagcgacg
     3661 gcggcgactg ggtgagcttc catcgccacc acggccggga cctcgagcgg ggcggcgtgg
     3721 ttcagaccgg tgagggcatg gggacctccg aggcgccgat gcgcaatcag atgaaggcgt
     3781 gggcgcaata catggcgaga gtgtgaagca tggcgactcc gagcaggggc aagaagaaag
     3841 caccgccgaa gcaagcgccc ggaacgtcgg cgaaaaagat gtcgatgcgc aaggcggccg
     3901 ggaggccgtc accgaaggcg gcgcgcgcgc agcgccgggc gggcgcggcg gccgacctgc
     3961 atcacgaaat cgagcagttc ctctaccgtc aggccgagct gctcgacgcc aagcgctggc
     4021 aggactacat cgacctgttc gccgacgacg gggtctactg gatgcccgcc agcccgcagg
     4081 accgcacctg ggacggcgtg cccgcgatct tcgccgagga caagaacctg atgaccgtgc
     4141 gcatgaagcg cgtgctgcat cccgatgcct ggtcgcagcg cccgctgtgg ggcaccaacc
     4201 acgtggtggg caacgtgatc ctgcaggcgg cggacggcgc cccggacgag ctggtggtgc
     4261 gctcgcgttt tcacatgatg gagctgcggc gtgacaacgt gcgtcatttc gccggcgcct
     4321 acacccacca tttgaagcgc acaccggacg gcctgcgcat caaactccag cgtgtggaca
     4381 tgaccaatgc ccaggcggcc tacgattacg tgctgcaagt ctgggtctag cgggagcgcc
     4441 gcatgaaagc cctccggtac gctctgtacg ccgtcggcgc cttggcgctg ctgctgatcg
     4501 tggcgatcgg cgtgctggcg gcgaccttcg atcccaacga ctacaagccg cgcatcgtcc
     4561 agctggtgca cgacaagacc ggccgcacgc tgtccatcgc cggcgacatc ggcctcaagg
     4621 tattccccaa gctcggcgtg cagctcggca agctgtctct gtccgagcgc gacggcagcg
     4681 gcgagttcgc gcacctggag gcggcgcagg tgtacgtcgc gctgctgccg ctgctgtcca
     4741 accgtgtggt ggtggacgaa gtgcgcgtcg atggcttgcg cgcgaacctg atcaagttcg
     4801 aggatgggag caccaatttc gacgatctgg ccaagggcgg gcagacgccg tccgaaacac
     4861 cggcgccgtc cgagacgccg gccgggcggc cgatcgagct cgacgtggac ggcatccgcg
     4921 tcaccgactc gcgcctgacc tggcaggacc gcaccagcgg caacgatctg gccatccaca
     4981 tcgccgagtt caagaccgga cgcatcgccg acaagcgccc cggcgacatc caactgtccg
     5041 ccgccgtgca gggcgccaag ccgcgcgtgg acctgcgcat cggcctggag tgcaggctca
     5101 ccttcgatct cgagcaacag ctctatcgac tgcagaacct ctccgcgaac ctggaaggtg
     5161 cggcgctgga cttcaccgac atcgcggtga aggtgggcgg cgatctggaa gcgctggcgg
     5221 cgcagcgtcg cgtcgcgttg tcggacctgc gcctcgaggg gaaggccgct cgcggcacga
     5281 accgctacaa cctcgacctg tccgcgccgg cgatcgaatc caccccggag gccttggccg
     5341 tgcagggctt gtcgctggcg gccaccggtt cgctgggcgc catgcaactc acgcaaagca
     5401 gcatcaaggc gccgacagtg cgcgtgaact tggcccgcaa cgagatgctg gtcgagggcc
     5461 tcagcctttc ggccagggcc aggcaggacg gggacaacct cgacatcggc ctgagcgccc
     5521 cccggctgca agtcacgccg gacagcgccg gcggcgagtc gatggtgctg accttgaagc
     5581 tcgatggcgc gcagcgcggc gccgacgcga ctgtgcgcgt gtcgggcctg gaaggttcgg
     5641 cgcgggcgct caagatccag tcgctcgcgc tggagctgga cgccaggcag caggacaacg
     5701 ccatcaaggg gcgcctgacc acgccggtga ccggcaacct ggagacgagg gtgttcgagc
     5761 tgcccaagct ggcgggtgat ttcaccgtca ccggccccac gctccccggc aagcgcctgc
     5821 aagtgccgct gagcgggctg gtgcgcgcgg acctcggcaa agagcgggtg atcggcgaca
     5881 tcgccacgcg cttcgacgag agcaacatca aggcaaaggg cggcatgacc ggcttcgctg
     5941 cgccggcata cgacttcgac gtggcgatcg acgcgctcaa cctggacaag taccgcgcgc
     6001 ccgagaagaa agaaacgacg gctgcgcaag gcccgcagcg cgacgagccg atcgatctgt
     6061 cggcgctcaa gggcctgaat ctgaagggcg aggtgcggat cggccggctg caggcgagca
     6121 acgtcaaggc ggccaacctg cgcgtgagcc tgcgggccag caacggcaag ctcgacgtcg
     6181 atccgctgtc cgcgaatctg taccagggca cggtcaaggg ggcgatcggc gtcgacgcca
     6241 acggcaacca gttcgcggtg aaccagacgc tcaccggtgt ggccatcggt ccgttgctgc
     6301 gggacgcggt gcaaaaggac attctcgagg gcaagggcag cgtggcgctg aatgtgacca
     6361 cgcgcggtgc cacggtgagc gagctgacgc gcgcgctcga cggcaccgcc cgcactgtgc
     6421 tgcgcgacgg cgcggtgaag ggcatcgatc tggcgggcac cgtgcggcgc gtgaaaacca
     6481 agctgacggg cggggacgtg gaaggcagcg ccgcccaggg cgagaagacc gacttctcgg
     6541 agatgtccgc cagtttcacc atccgcaagg gcgtggcccg caacgaagat ctcgatctca
     6601 agtcgccctt cctgcgggtc accggcagcg gcgaggtcga catcccgcag tcgcgcctcg
     6661 actatgtggt caagacctcc gtggtgggca cgacggccgg gcagggcggc aaggagctgt
     6721 ccgagctgcg tggcctcacc atcccggtgc gcgtggccgg gcccttcgac aagctcggct
     6781 acaaggtgca gttcagccag atggtgcgcg ggtcgacgaa ggagcagctg gaggcggcga
     6841 agcgtacggg caaggaggcg ctcgaggggg cggcgcgcgg caagctcgag acgctgctgg
     6901 gcggccccgg cggaccggag gaggcgaagg agggtcagcc gggcggcgag caggcgcacc
     6961 aggccgcgcc caaacggccg caggacgaag tgaaggacaa gctcaaagag ctgctccgtt
     7021 gaacgccggc gcagtgggtc ccagcttcgc ggcgcgcctc atcgcctggc agaagacgca
     7081 gggccgtcat gagcttccct ggcagagcac ccgcgatccc taccgcatct ggctctcgga
     7141 aatcatgctc cagcagaccc aggttgctgc ggtcgttcct tactacgagc gattcctggc
     7201 gcggcttccc gacctggcgg cgctcgccgc cgcgcaggag gacgcagtgc tggcgctgtg
     7261 ggcggggctc ggctactacg cgcgcgcgcg caacctccac cgcgcggccc agacgatcgt
     7321 gcagcggcac cgcggcatct tcccgcgcga gctggacgcg gtgcgcgcgc tgcccggtgt
     7381 cgggcgctcc accgccgccg cgatctgcgc cttcgcctac ggcacgcggg cggcgatcct
     7441 cgacggcaac gtcaagcgcg tcctgacgcg ccacttcggc atcgacggct atcccggcga
     7501 gcggcgcatc gagaacgctc tgtggcggtt atcggagtcg ctgctcccgg cgcgggacgt
     7561 cgaggcctac acgcagggtc tgatggatct cggggccacc ctgtgcgtgc gcggcaatcc
     7621 gcgctgcggc gcgtgcccgc tgcaggacgg ttgcgtcgcg cggcgcgagg gccggaccca
     7681 agactggccc acggtgcgtc cggccaaggc gctgccgcag cggcgcacca cgatgctggt
     7741 gctggtgagc cgcggcgagc tgttgctgga gaagcgtccg gcgtcgggca tctggggcgg
     7801 cctttggtgc tttcccgaga tcgacggcgc ccggttcgat cgggaagctg ggcttgactg
     7861 ggaatccggg ctcgagcgcg aatgcctggc gcgtttcggc ttgcatgccg gcgcgtcgcg
     7921 tcgcctgcgc gacatcgagc acggcttcac ccacttccgc ctgcgcatca cgcccgtgct
     7981 cgtgcgcacc gaccgcgcgc cgggcgccgg cgagcccggc agcctgtggc tgacgccggc
     8041 ggacgcgcgc ggggccgcga ttcccgcgcc ggtgcgcaag atcatcgacc tcatagaagt
     8101 gaatcgtgat tcgtgattcg tgccccggat tccgctgcgc tgcatccggg ctacgcacat
     8161 caccaatcac gattcacgag tcacgaatca cggcttcaca ccgccaccga ccggctctgc
     8221 aggaacgtgc gcaaccgctc gaggcgcgct tgcgggtcgt gcagctccag catccgctgt
     8281 ttgagctcga cttcgagcgg cagcacctcg gccaggcggc aggccaccca gtcggcgtcg
     8341 tccaggcgca ccggtgacgg gaagttgtcc gctccgacct tgtcgatgat cttcgcgagc
     8401 aggtccgcca ggggccggta ggagggcgcc agcgggcgcg acggcgacgc gtcctgcagc
     8461 tccaccagcc cgtagagcaa gccgctgcgc accgcccagt gttcggcgat gcggaacacc
     8521 gattggcccc ggcacagcag gtggaacaac cccaggtggg gcatgtccca atcccggatc
     8581 agggcggtgc agccgatcgg gtagggcacg gcgggggcgc cgacctcggc gccctcgcgc
     8641 agcaggcaca cgccgaacgg gctggcgtcg cggatgcatg tcttggtcat gtccaggtag
     8701 cgctgctcga agatcttgag cggcagcagg ccccccggga acagcacggt gcgcagggga
     8761 aagatcggga tctcgagggc catgatcctc gcccgggctc acgcctgcgg cgcggcttcg
     8821 ccccagcggg gcgtcagact gtgcgcgatc ccgagctggt cgagcacgcg cgccacgacg
     8881 aaatccacca ggtcctccac gcgccgggga tggtgataga agcccggatt ggcgggcagc
     8941 acgacggcgc cggcgcgcgc caggcgcagc atgttctcca gatggatcgc ggaaagggga
     9001 gtctcgcgcg gcaccagcac cagcttgcgc gcctccttga gcatcacgtc cgcggcgcgc
     9061 tcgatcaagt tgtcggccag gccttgtgcg acggcggcca gcgtgcccat gctgcacggg
     9121 cagatcacca tcgcgtcggg gggattggag ccggaggcca ccggcgcgaa ccattcctcg
     9181 cgcccgaagg cggtgagctg ccccggctgc gctcggaaac gcgcgccgaa gaaggtctcg
     9241 gcctccttcg cccgccccgg caaggcgagg tccatctcct ggcgtgcgac gatctgcgag
     9301 gcctgcgaat acagcaggta cacgcgcgcg cccgaggcga gcaggcattc cagcaggcgc
     9361 accccgtagg gcatccccga cgcgccggtg aacgccaccg cgacggtgcc gttcattgcg
     9421 cctctccggt gtccggcggc ggggcggacc cggcatcggc gggggcggcg gtgctcagct
     9481 gctctttcag ctcgcgcagg cgcgcctcca ggcgttcgac ttcggtgcgc agcgcttccg
     9541 ggctggcggg caaggcatcc tcggcctgcg cgcgggaagc ctggtcctgc tcgacgatca
     9601 ggcacatcag cgtctcgaag cggtcgccaa cggtttccgg gatgatcggc tgcgacggcg
     9661 cctccggcgt gtacacgttc tccaggatcg cgccgtcctg ggcgtagagc agatagccca
     9721 gcgtgcgata ggagtgccgc gcgcaatcga cgcgctcgcg gtacatggcc atgcgcgcct
     9781 tgcccgaccg cgccggctga ggcgtgcgga acagcacgcg ccgccagtac gtgatttcgt
     9841 ccttctcgat ggtgagcttg ctgcggtcgt agaagtgttg gtgctggtcg ggggtgccca
     9901 cccgcaccca gtccgccgcc tgcgccgcgg ccgacagcga caatgcaagc acgccggccg
     9961 ccaggcgttt cgtgggagac ggatgcgggc tgatcaacac gcaggcacag aggcgcggac
    10021 aaagaacgcc attatccgtt tttccctgcg cctctgcgtc tccgcggtga acgcagcggt
    10081 cactgcgcca gctcgcgatg acgcacgatc accggtaggg tcggctcgaa gcgccgggcc
    10141 agcacctcgc tgaagtacac cgagcggtgg cgcccgccgg tgcaaccgat ggccaccgtg
    10201 agatagctgc ggttgtcgcg caggtagcaa ggcaaccacg cgtcgagaaa cgcggagatg
    10261 tcggccagca tcttctgggc gtcggcatcg gcttccatga acgcgatcac ctggtcgtcg
    10321 ttgccggtga gcggttgcag caccggatcg tagtgcgggt tgggcaggca gcgcacgtcg
    10381 aacaccagat cggcatccag cggaatgccg tgcttgaagc cgaaggactg gaacagcagg
    10441 gtcatgccgc tgccggcgag cccggcgaag tccttgaccc aggcgcgcag cgcgttggtg
    10501 ttgagctcgc tggtgtcgat gcggtgcgcc agggcgacga tatcttgcag catcgcgcgc
    10561 tcggcgtcga tgcattccgg cagggtccgc gcgtcgtccg acagcggatg gcggcggcgc
    10621 gtctcggaga agcgcttgac cagcgtgccg gtcttggcgt cgaggaacag cacgcgcagg
    10681 tccacctggc gccggcgcag ctcgaccacc gacgcgggca gttccttgag cgtggggccg
    10741 ctgcgcacgt ccacgctcac tccaacccgg gcgtaacccg attgcgccag gaaatcgacg
    10801 acctcgacca gcaggttcgc cggcagattg tccacgcagt agtagccgat gtcctccagc
    10861 acgttgagcg cgatgctctt gcccgagccg gacatgcccg tgaccaggat cacgcgcatc
    10921 tgcgctgcgc ccgctgcggc aggggccgcc gcggccagcg gcaggccgag ctcagggttc
    10981 gcggccaaag tgctgctcct ggcgctggat gaactcctgc gtgctgtcga tgccgcgcag
    11041 ctgcagcacg tagttgcgca ccgccgcctc caccagcacc gccaggttgc ggccggccgc
    11101 caccggcagc accaccttgc gcaccttcac ccccaggatc tcctcgaagc tcgcggtgag
    11161 cggcaggcgc tcgagcccgg ggatcacgcc gccgaccggg cgctccagct gcacgatcag
    11221 cttcagattc ttgcgcacgc gcaccgccgt ctcgccgaag atggtgcgga tgttgagcat
    11281 gcccaggccg cgcacttcga ggaagtcctt gagcagctcg gggctgcgcc cctcgagcgt
    11341 ctccggcgcg atgtgataca gctccaccac gtcgtcggcc accaggccgc cgccgcgcgt
    11401 gatcagctcc agcgccagct cgctcttgcc cacgccgctg tcgccggtga gcagcacgcc
    11461 catgccgagg acgtcgagca gcacgccgtg gcgggtgacg aactcggcca gcgcgcggcc
    11521 catgtaggtg cggatcaccc agatgatctg caggctcggc agcggcgagc cgatgagcgg
    11581 cgcgttcatg cgctcggcga agcgcctgag cgtgtcgggc gcgcgctcgc cgtcgctgac
    11641 gatgacgcag gcgagcccgc tggcgtgcag ccgcgccagg gcctgctcgc agtcggcctc
    11701 gcccatcgcc gagaagtagg cggcctcggt cgggctgaag atctggatcc agttcgggtg
    11761 gtagaagttg aggtgcccga tcagcccctc gttcgcctga tagatgcggc gcgtgtcgag
    11821 cgcgttgccg gcatgcgtct cgccgcccag ccaggacagc ttgagcgcct cctggttgtc
    11881 cttgaacagc tgcgctacgc tgacgagcgg catgcgggtc tccgtgcagc gcgggctagt
    11941 tggccgtccg ggcctgccag ccggcgatca ggcgctgcgc ggccagcgcg tcgggggccg
    12001 cggccagggc ggcccgcatc tcgcggtcgc tgaacatttc cgcgagctga ctcaggatct
    12061 cgaggtgtgc ctcggtggac ttctcgggca ccagcagcac gaagatcagg cgcaccggct
    12121 cgtcgtccgg tgcatcgaag gcgatcgggg cgcgcgtgcg caggaagcag gccacggcgg
    12181 cgagcagccc cttgacccgt ccatgcggga tggcgatgcc ctggccgagg ccggtggagc
    12241 ccatccgctc gcgcgcgagc aggctgtcga ggatgtgccc gcgcttgatg ccggtcttgc
    12301 cctcgagcag ggtggccgcg tgctcgaaca ccgcccgctt gtcctgcgcc gcgatatcga
    12361 gcgcgacgtg ggaggtgggg agcagcgagg cgagaaggct gatggcgggt gccacccgag
    12421 tcgccgtggc cgaagctgaa gcgcgctccc ggcgctcacc cgcccgcgcg gtggcggttg
    12481 gctccgttgt agcgctgctc gacggccttc tccttgtgct tgaggatctg gcgatcgagt
    12541 ttgtcgacca gcccgtcgat cgcggcgtac atgtccgcgt cgatgctctc gcagaagatg
    12601 tcgcggccgc gcacgtgcac ggtcgcctcg atacgctgct gcagcttgtc caccgacatg
    12661 atcacgttca cgtcgatgac gtggtcgaag tggcgcgtga tgcgctccag cttggaggtg
    12721 acgtagctgc ggatcgcggg cgtgatgtcg aggtggtgcc cggtcagttg cagattcatc
    12781 tagcggctcc ttgtgcttgt tcgagtcaga tcgacttcct cagattgacc ggcaggatct
    12841 gcagagattc cctgtacttg gcgatggtgc gacgcgccac gacgatgccc tgcttgccca
    12901 ggatctcgga tatctggctg tcggacagcg gcttgcgcga gtcctcctcg gccaccagct
    12961 gcttgatcag cgcgcggatg gcggtggccg agcaggcgcc gccggtctcc gtggtgacgt
    13021 ggctgccgaa aaagtacttg agctcgaaga tgccgcgcgg ggtgagcatg aacttctgcg
    13081 tggtgacccg cgaaaccgtg gactcgtgca ggcccagggt gtcggcgatc tcgcgcagga
    13141 cgagcgggcg catggccact tccccgtgct cgaagaagtg gcgctggcgg tcgacgatcg
    13201 cctgggacac gcgcaggatg gtgtcgaagc gctgctgcac gttcttgatc agccacttcg
    13261 cctcctgcag ctgcccggcg agctgctgca ggctggcgtc gcggttgcgg tgcaggatgt
    13321 cggcgtacat gcggttgatg cgcagcttcg gcatcgcatc cgggttgagc gatgcgcgcc
    13381 acaggccctt gaccttcttc accaccacgt cggggacgat gtagcgcgcc tcctcgccgg
    13441 agaagttggc gcccggccgc ggattgagac tggtgatcag actctgggtc ccgcgcagca
    13501 cctcgtcgct gcacttgagc agcttcttca gccgcgcgta gtcgcgcgag gcgagcaggt
    13561 ccagatggtt gcgcaccgtg gtcagcgcct cggcgcggta gggcgtgtcg cccggcatgg
    13621 cctcgagctg cagcgccagg cactcggaca gatcgcgcgc accgatgcca gccggttcga
    13681 gctgctgcag gtgccgcaag ccgatctgca ggtcctcgcg gtcgatgccc gcctccgcag
    13741 gcagcatggc agcgagctcg tcgaggtcct gcgtgaggta gccgtcgtcg tcgagcgtgt
    13801 ccaccagcat ggccacgatc ctcttgtcgc ggtccgagca cttcaggagg ttgacctgcc
    13861 acagcagg
//
LOCUS       DASPGE010000059         3676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1659074, whole genome shotgun sequence.
ACCESSION   DASPGE010000059 DASPGE010000000
VERSION     DASPGE010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659074"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..501
                     /locus_tag="VD791_01885"
     CDS             97..501
                     /locus_tag="VD791_01885"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXV06751.1"
                     /translation="MTALVTHRGGCHCGRVRFEVDAPYDLDATYCNCSICSKTGYLHL
                     IVPAARFRLLTPRDELITYTFNTGVAQHYFCRTCGIKSFYVPRSNPDGFSVNVRCLDE
                     GSFGEVRITDFDGRNWEAHGSKLAELTRMPGA"
     gene            506..1435
                     /locus_tag="VD791_01890"
     CDS             506..1435
                     /locus_tag="VD791_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HXV06752.1"
                     /translation="MILLFQSDTDDPGPWRSALVRALPGLEFRVWPEVGRPEEIDYAL
                     VWRPPPGMLKSLPRLKAILSLGAGVDHIFGDPELPRGVPITRMLDAGLAAQMSEYALY
                     GVLHFHRHMGRYAEQQRSGVWRQLPAVPPQQRTIGVLGLGVLGADFARKAVALGFRVL
                     GWSRSPTQIAGVEAFHGEAGFEPFLARSEVLVNFLPLTRDTEGILDARTFARVRRGAF
                     LINIARGAHLVEPDLLAALDSGQLGGAMLDVFAHEPLPADHPFWRHPRILVTPHIAGL
                     AIAELMVEQVVDNIRRIEREETPLGLVDPERGY"
     gene            1513..2958
                     /locus_tag="VD791_01895"
     CDS             1513..2958
                     /locus_tag="VD791_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXV06753.1"
                     /translation="MATQQRSNYIAGEWVTGASASRDVNPSDVSDIVGEYAQADQKQT
                     EQAIAAARAAAPAWAAFNVQQRADALDRIGAEILARKDELGTLLSREEGKTLPEGTGE
                     VVRAGNIFKFFAGEVLRRAGELVPSVRPGIDVEITREPVGVVGIISPWNFPAAIPAWK
                     VAPALAYGNCVVFKPADLVPGSAWAMAEIISRAGLPAGVFNLVMGRGSVVGETMIGDP
                     RVDAISFTGSVDTGRKVALKAVAQLKKLQLEMGGKNPLVVLDDADLKTAVESAVNGAY
                     FSTGQRCTASSRLVVTKGIHNKFVEALIERIKAMPVDHALKPGTQMGPVVDKNQLNQD
                     LFYIEEGKKEGAKLAHGGSLLKREKDGFYLEPALFTETRNDMRINREEVFGPVATVIA
                     ADGYDEALAIANDTQFGLASGIITTSLKHAAHFRRNSQAGMVMVNLPTAGVDYHVPFG
                     GRKGSSYGPREQGRYAAEFYTAVKTAYVAAG"
     gene            3073..>3676
                     /locus_tag="VD791_01900"
     CDS             3073..>3676
                     /locus_tag="VD791_01900"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HXV06754.1"
                     /translation="MNDLTQTPRSRVKRLPKRGHYDFDTAAAILDAGFLCHVGYVIDG
                     KPYVTPTSYWRERDHVYWHGSSASRMLRTLEDGVDACLTVAHVDALVLARSAFHHSIN
                     YRSVMLFGKARKVEDAAAKERALHNFVERMYPGRWQELRPVHPQELKATTVLHMQIGE
                     GAAKIRTGPPIDDAEDYGWPAWAGVVPVRSVAGAPQDDGRL"
BASE COUNT          595 a         1216 c         1321 g          544 t
ORIGIN      
        1 ctggccggtg cgggacttcg tgctggtgcg ctcggagctg aacgacgagg gctcgcggta
       61 cactatcgtg gagcgctgga agggggagga ggaatcatga cggctctggt gacacatcgc
      121 ggcggctgcc actgcggccg cgtgcgcttc gaggtggacg ccccgtacga tctggacgcg
      181 acctactgca attgctcgat ctgctccaag accggttacc tgcacctgat cgtgcccgcc
      241 gcgcggtttc gcctgctcac gccccgggac gagttgatca catacacgtt caacaccggc
      301 gtggcccagc actacttctg ccgcacctgc ggcatcaagt ccttctacgt gccgcgctcg
      361 aaccccgacg gcttcagcgt caacgtgcgc tgcctggacg agggcagctt cggcgaggtg
      421 cgcatcaccg atttcgacgg ccgcaactgg gaggcgcacg gcagcaagct ggcggagctc
      481 acgcgcatgc cgggcgcctg aaggcgtgat cctcctgttc cagagcgaca cggacgaccc
      541 cgggccctgg cgcagcgcgc tggtgcgcgc ccttcccggg ctcgagttcc gcgtctggcc
      601 cgaggtgggc cgtcccgagg agatcgacta cgcgctggtg tggaggccgc cgcccgggat
      661 gctgaagagc ctgccgcggc tcaaggcgat cctgtcgctg ggggcgggcg tggaccacat
      721 cttcggcgac ccggagctgc cgcgcggcgt gccgatcacg cgcatgctgg acgcggggct
      781 tgcggcacag atgtccgagt acgcgctgta tggtgtgctg catttccacc gccacatggg
      841 ccggtacgcc gagcagcagc gcagcggcgt gtggcgccag cttccagcgg tgcccccgca
      901 gcagcgcacg atcggcgtgc tgggcctggg cgtgctgggg gcggacttcg cgcgcaaggc
      961 ggtcgcgctg gggttccgcg tgctcgggtg gagccggtcg ccgacacaga tcgccggcgt
     1021 cgaggcgttc catggagaag cgggattcga gcccttcctt gcgcgcagcg aagtcctggt
     1081 caacttcctg ccgctgaccc gcgacaccga aggcatcctc gacgcccgca ccttcgcgcg
     1141 cgtgcgccgc ggtgcgttcc tgatcaacat cgcgcgcggc gcgcatctgg tcgagcccga
     1201 tctgctggca gcgctggaca gcgggcagct cggcggggcg atgctcgacg tcttcgcgca
     1261 tgagccgctg cccgccgacc atccgttctg gcgccatcct cgcatcctcg tcacgccgca
     1321 catcgccggg ctggccatcg ccgagctgat ggtggagcag gtggtggaca acatccggcg
     1381 catcgagcgc gaggaaacgc cgctcgggct ggtcgatccg gaaaggggat actgagccgc
     1441 cgctgagtgc ggcggcacgc ggcggtagaa tagcgtcttc cgcggacgcg accgaccccg
     1501 gggggagagc acatggctac acagcagcgt tccaactaca tcgcgggcga gtgggtgacg
     1561 ggtgcgagcg ccagccgcga cgtcaacccg tcggatgtga gcgacatcgt cggcgagtac
     1621 gctcaggccg accagaagca gaccgagcag gcgatcgccg cggcgagagc ggcggcgccg
     1681 gcttgggcgg cgttcaacgt gcagcagcgc gccgacgcgc tggaccgcat cggcgcggaa
     1741 atcctggcgc gcaaggacga gctgggcacg ctgctctcgc gcgaggaagg caagacgctg
     1801 ccggagggca ccggcgaagt ggtgcgcgcg ggcaacatct tcaagttctt cgccggcgag
     1861 gtgctgcgcc gcgccggcga gctggtgccc tcggtgcgcc ccggcatcga cgtggagatc
     1921 acgcgcgagc cggtcggcgt ggtggggatc atctcgccct ggaacttccc ggccgccatc
     1981 cctgcgtgga aagtggcgcc cgcgctcgcc tacggcaact gcgtggtgtt caagcccgcc
     2041 gacctggtgc cgggctcggc ttgggcgatg gcggagatca tctcgcgcgc tgggttgccg
     2101 gcaggcgtgt tcaacctggt gatggggcgc ggcagcgtgg tgggcgaaac catgatcggc
     2161 gatccgcggg tggacgcgat cagcttcacc ggctcggtgg acaccggccg caaggtggca
     2221 ctgaaagcag tggcgcagtt gaagaagctg cagctcgaga tgggcggcaa gaacccgctg
     2281 gtggtgctgg acgacgccga cctcaagacc gcggtcgagt cggcggtgaa cggcgcctac
     2341 ttctccaccg gccagcgctg caccgcttcg tcgcgcctgg tggtcaccaa gggcatccac
     2401 aacaagttcg tcgaggcgct gatcgagcgc atcaaggcca tgccggtgga ccacgcgctc
     2461 aagccgggca cgcagatggg gccggtggtg gacaagaacc agctcaacca ggacctgttc
     2521 tatatcgagg aaggcaagaa ggaaggcgcc aagctcgccc acggcggcag cctgctcaag
     2581 cgcgagaagg atggcttcta cctggagccg gcgctgttca ccgagacgcg caacgacatg
     2641 cgcatcaacc gcgaagaggt gttcgggccg gtggccaccg tgatcgcggc cgacggctac
     2701 gacgaggcgc tggccatcgc caacgacacg caattcggcc tggcctcggg gatcatcacc
     2761 acatcgctca agcacgctgc gcatttccgc cgcaacagcc aggccggcat ggtgatggtg
     2821 aatctgccca ccgccggggt cgactatcac gtgccgttcg gcgggcgcaa ggggtcgagc
     2881 tacgggccgc gcgagcaggg gcggtatgcg gcggagttct acaccgccgt gaagaccgcg
     2941 tacgtggcgg cggggtgatg cgatgctact cgtagcccgg atgcaggccg caggccggaa
     3001 tccgggaaac gccccatctt ccccagattc cgctacgctg catccgggct acgcaacttc
     3061 tcgcatacat cgatgaacga cctgacccag acgccacgct cgcgcgtgaa gcgcctgccg
     3121 aagcgcgggc actacgattt cgacaccgcg gccgcgatcc tcgacgcggg gttcctgtgc
     3181 cacgtcggct acgtgatcga cggcaagccg tacgtgacgc ccaccagcta ctggcgcgaa
     3241 cgcgaccacg tctactggca tgggtccagc gcgtcgcgca tgctgcgcac gctcgaggac
     3301 ggcgtggacg cctgcctgac ggtggcgcat gtggacgcgc tggtgctggc gcgctcggct
     3361 ttccaccact cgatcaacta ccgctcggtg atgctgttcg gcaaggcgcg caaggtggag
     3421 gacgcggccg ccaaggagcg ggcgctgcac aacttcgtcg agcgcatgta tcccggccgc
     3481 tggcaggagc tgcggccggt gcacccgcag gagctgaagg cgaccaccgt gctgcacatg
     3541 cagatcggcg aaggggcggc caagatccgc accgggccgc cgatcgacga cgcggaggac
     3601 tacggctggc cggcctgggc cggtgtggtg ccggtgcgca gcgtggccgg cgcgccgcag
     3661 gacgacggcc ggctgg
//
LOCUS       DASPGE010000060         4041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_370525, whole genome shotgun sequence.
ACCESSION   DASPGE010000060 DASPGE010000000
VERSION     DASPGE010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370525"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2191)
                     /locus_tag="VD791_01905"
     CDS             complement(<1..2191)
                     /locus_tag="VD791_01905"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HXV06755.1"
                     /translation="MNSRLLALVVGALVVGAAAMLCAPAAWSMRATEVAPCRPNMPQE
                     VRSFAGLFTVHVVCDHVLFEIPPKLLNRDMLLNTEFSGLSAGVEKLAPGAVVDSRVVR
                     WVRRGNKVFLENVRYEMRAENTSGLDRGVEATQINTVGVVFEAVAEGKDGAPIIDVTT
                     LFTETPRGFAMEFKRYFDMSTNDPKRSYIDSVKVFPQNIEVGFHQTWVPDPKEFEDSY
                     KPGRTPIKAALGFSFHTSMLLLPETPMVGRYSDPRVGYFEVEFQDYGTTENRGVKRGF
                     IMRYRLEKKDPMAEVSDPVKPIVFYISNEVPEKWRPYLKKGIEAWQGPFEKAGFRNAI
                     IARDAPTLEQDPDWDPEDVRYSVIRWTPSPRENAMGPAVVDPRSGEVLSSHAIFWHDI
                     LRLAETWYFTQVGALDPRAHKLPLPDEVMGEILAYVTTHEVGHALGLRHNFKAPSAVS
                     VQQLRSREWTAKWGTSTSIMSYARFNYVAQPGDKAELFPKFGQYDYFAIDWGYRQFGG
                     GHMAGDPMGCDDEWPMLDSMAATQIKDRLLHFGGEDAIAPLDPSINTNVLSSDPVAAA
                     DLGLRNIDRTAALLVPATTRLGRDYALLADMYYALVQQRHRELAAVAKIVGGVEETRY
                     QGGRGGTPFQPVSPARQREAVRFLIDRAFATPEPLLNREILGRITPTGGDDALQGSNI
                     RLLQQLLDPAVFVRMVEASTADKKTKPYMGLDLLEDLNRGIFNELAAD"
     gene            2498..2953
                     /locus_tag="VD791_01910"
     CDS             2498..2953
                     /locus_tag="VD791_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016723725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXV06756.1"
                     /translation="MTASIKITVAAVVQRDGRFLLVEEETDAGLRLNQPAGHLEPGES
                     LTNGVARETLEETAYRFVPQALLGVYRWQQPTGITYVRFTFLGEATGPEPGRTLDQGI
                     VRALWLTPEALRAERARHRSPLVLRCVEDCLAGRRYPLELITDYPGVTA"
     gene            2950..4014
                     /gene="mnmA"
                     /locus_tag="VD791_01915"
     CDS             2950..4014
                     /gene="mnmA"
                     /locus_tag="VD791_01915"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HXV06757.1"
                     /translation="MTGSRVVVGMSGGVDSSVAAWLLKEQGHDVLGLFMKNWEDDDSD
                     EYCTSRADLIDAVSVAELIGIDIEAVNFSAEYRERVFANFLAEYRAGRTPNPDVLCNA
                     EIKFKAFLDHALQMGADRIATGHYARVREVDGRLELLKAADASKDQSYFLYRLSQAQL
                     ARTLFPLGEMLKRDVRALARRIGLPNHAKRDSTGICFIGERPFREFLMRYLPTAPGPM
                     ETPDGRRVGTHHGLMYYTIGQRHGLGVGGPGEAWYVAHKEVESNRLVVVQGHDHPALF
                     KDRLRAGQLSWVAPAAPAAPAPYAAKTRYRQADAPCVLTQVDTGACELSFERPQWAVT
                     PGQSAVLYDGEVCLGGGIID"
BASE COUNT          613 a         1387 c         1381 g          660 t
ORIGIN      
        1 tgtcggccgc gagctcgttg aagatgccgc ggttgaggtc ctccagcagg tccaggccca
       61 tgtagggctt ggtcttcttg tccgcggtgg aagcctcgac catgcgcacg aacaccgccg
      121 gatccagcag ctgctgcagc agcctgatgt tcgagccctg cagcgcatcg tcgccgccgg
      181 tgggggtgat gcggcccaag atctcccggt tcagcagcgg ctccggggtc gcgaacgcgc
      241 ggtcgatcag gaagcgcacc gcctcgcgct gccgtgccgg cgagaccggc tggaacggcg
      301 tcccgccgcg gccgccctgg tagcgcgtct cctcgacgcc gcccacgatc ttggcgaccg
      361 ccgcgagctc gcggtgccgc tgctgcacca gcgcgtagta catgtcggcg agcagcgcgt
      421 agtcgcgtcc caggcgcgtg gtcgcgggca ccagcagcgc cgcggtgcgg tcgatgttgc
      481 gcagcccgag gtcggccgcc gccaccgggt ccgagctgag cacgttggtg ttgatgctgg
      541 gatcgagcgg agcgatcgca tcctcaccgc cgaagtgcag cagccggtcc ttgatctgcg
      601 tggccgccat gctgtccagc atcggccact cgtcgtcgca gcccatcggg tcgccggcca
      661 tgtggccgcc gccgaactgg cggtaacccc agtcgatggc gaaatagtcg tactggccga
      721 acttcgggaa cagctcggcc ttgtcgccgg gctgcgccac gtagttgaag cgcgcgtagg
      781 acatgatcga ggtcgaggtg ccccacttcg ccgtccactc gcgactgcgc agttgctgga
      841 cgctcaccgc cgagggcgct ttgaagttgt ggcgcaggcc cagcgcatga ccgacctcgt
      901 gcgtggtcac ataggccagg atctcgccca tgacttcgtc gggcagcggc agcttgtgcg
      961 cgcgcggatc gagcgccccg acctgggtga aataccaggt ctccgccagg cgcaggatgt
     1021 cgtgccagaa gatggcgtgg gacgagagca cctcgccgct gcgcggatcg accaccgcgg
     1081 ggcccatcgc gttttcgcgc gggctgggcg tccagcggat caccgagtag cgcacgtcct
     1141 ccgggtccca gtccgggtcc tgctccagcg tgggcgcgtc gcgcgcgatg atcgcattcc
     1201 tgaatcccgc cttctcgaac ggcccctgcc aggcctcgat gcccttcttg aggtacgggc
     1261 gccacttctc cggcacctcg ttgctgatgt agaacacgat cggcttgacc gggtcggaca
     1321 cctccgccat cgggtccttc ttctccagcc ggtagcgcat gatgaagccg cgcttgacgc
     1381 cgcggttctc ggtggtgccg tagtcctgga actcgacctc gaagtagccc acgcgcggat
     1441 cggaatagcg ccccaccatc ggcgtctcgg gcagcagcag catgctggtg tggaacgaga
     1501 accccagcgc cgctttgatc ggcgtgcgcc cgggcttgta cgagtcctcg aactccttcg
     1561 gatccggcac ccaggtctga tggaagccga cctcgatgtt ctgcgggaac accttgacgc
     1621 tgtcgatgta ggagcgcttc gggtcgttgg tgctcatgtc gaagtagcgc ttgaactcca
     1681 tggcgaagcc gcgcggcgtc tcggtgaaca gcgtggtcac gtcgatgatc ggggcgccgt
     1741 ccttgccttc ggccaccgcc tcgaacacca cgcccacggt gttgatctgg gtggcttcga
     1801 ccccgcggtc gagcccgctg gtgttctcgg cgcgcatctc gtagcgcacg ttctccagga
     1861 acaccttgtt gccgcgccgc acccagcgca ccacgcggct gtccaccaca gcacccggcg
     1921 ccagcttctc caccccggcg gacaggcccg agaactcggt gttgagcagc atgtcgcggt
     1981 tgagcagctt gggcgggatc tcgaacagca cgtggtcgca gaccacgtgc acggtgaaca
     2041 gcccggcgaa gctgcgcacc tcctggggca tgttcgggcg gcagggggcc acttcggtgg
     2101 cgcgcatgga ccaggcggcg ggcgcgcaca gcatggcggc cgcgcccacc acaagagcgc
     2161 ccaccacaag agctaggagt cgagagttca tagcggttat catgagagcc ggttgcatgt
     2221 tcggtcgttc gatgtggcga tagcttacac aaggcgccgc tcctgcgccg ggcgggtccg
     2281 tttggattga cgcagcgcgg ggcaggccgt atcctttcgc cccttttccg tcgggcgccc
     2341 ttttccgtcg gcccggacgg caaaaaccct atacggcagg cgagcatgtg cggattcgtg
     2401 acccgggata gggcgcgcta gtccggcggg ccgccagccg cccgaaaggc agggccacgg
     2461 ccctgccttt ctcgcttccg ggaatcggga tatggccgtg accgcatcga tcaagatcac
     2521 cgttgccgcc gtcgtccagc gcgacgggcg cttcctcctg gtcgaggaag aaaccgacgc
     2581 cgggctgcgt ctcaaccagc ccgccggcca tctggagccc ggggaatcgc tgacgaacgg
     2641 cgtggcgcgc gaaacgctgg aagagaccgc ctaccgcttc gtgccgcagg cgctgctcgg
     2701 cgtgtaccgc tggcaacagc cgacggggat cacctacgtg cgcttcacct tcctcggcga
     2761 agccacgggc ccggagcccg gccgcactct cgaccagggc atcgtgcgtg cgctgtggct
     2821 cacccccgaa gcgctgcgcg ccgagcgggc ccgccaccgc agccctctgg tgctgcgctg
     2881 cgtggaggac tgcctcgccg gacggcgtta cccgctcgag ctgatcaccg actatccggg
     2941 ggtgaccgcg tgacgggcag ccgcgtcgtg gtcggcatgt ccggcggcgt cgattcctcg
     3001 gtcgcggcct ggttgctcaa ggagcaggga cacgacgtgc tcggcctgtt catgaagaac
     3061 tgggaggacg acgacagcga cgagtactgc acctcgcgcg cggacctgat cgacgcggtg
     3121 tcggtggccg agctgatcgg catcgacatc gaggcggtga acttctccgc cgaataccgg
     3181 gagcgggtgt tcgccaattt cctggccgag tatcgcgccg gccgcacgcc gaacccggac
     3241 gtgctgtgca acgcggagat caagttcaag gcgttcctgg atcacgcgct gcagatgggc
     3301 gcggaccgta tcgccaccgg acactatgcc cgggtgcgcg aggtcgacgg ccgcctcgag
     3361 ctgctcaagg ccgccgacgc gtccaaggac cagagctact ttctgtaccg gctcagccag
     3421 gcgcagctcg cgcgcacact gttcccgctg ggcgaaatgc tcaagcgcga cgtgcgcgcg
     3481 ctcgcccgtc gcatcggcct gcccaaccac gccaagcgcg acagcaccgg catctgcttc
     3541 atcggcgagc gcccgttccg cgagttcctg atgcgctacc tgcccaccgc ccccggcccg
     3601 atggagacac ccgacggccg ccgcgtgggc acgcatcacg gcttgatgta ctacaccatc
     3661 ggccagcgcc atggcctggg tgtcggcggg ccgggcgaag cgtggtatgt cgcgcacaag
     3721 gaggtcgaga gcaaccgcct ggtggtggtg cagggtcacg atcaccccgc cctgttcaag
     3781 gaccggctgc gcgcggggca gctgagctgg gtggcgcccg ccgcacccgc cgcgccggcg
     3841 ccgtatgcgg ccaagacgcg ctatcgccag gccgacgcgc cctgcgtgct gacgcaggtg
     3901 gacacgggcg cttgcgagct gtcgttcgag cggccgcagt gggcggtgac gccggggcaa
     3961 tcggcggtgc tgtacgacgg cgaggtttgc ctcggcggag gcatcatcga ctgaagcgcc
     4021 gcgcgctgcg tcgaccgcgg t
//
LOCUS       DASPGE010000061         4178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_488226, whole genome shotgun sequence.
ACCESSION   DASPGE010000061 DASPGE010000000
VERSION     DASPGE010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488226"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VD791_01920"
     CDS             complement(<1..216)
                     /locus_tag="VD791_01920"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656416.1"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HXV06758.1"
                     /translation="MSAGDVLVGLIGAGIRRSLSPALHEEEARHHGLRLQYRLIDLAD
                     GGENADDLPRLLATVRTLGFAGVNITHP"
     gene            complement(213..1145)
                     /locus_tag="VD791_01925"
     CDS             complement(213..1145)
                     /locus_tag="VD791_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HXV06759.1"
                     /translation="MSAVDIVSLGEPLYEFSALPGQDRPGQDHHWLQGFGGDTSNCAI
                     AAARQGAKVAYLTRLGQDEFGEQFLALWKEEGLDVSGVGIDPDAHTAVYFITHGPAGH
                     VFSYLRAGSAASRMRPEHLPLSLIRSAQFLHTSGITQAISASACDTAFAAMEAARQAG
                     VRVAYDANLRPRLWPAARARAVITAALGMSDLFLLSIDEAQALCDLDQPDAVLDWCHR
                     RGARLVALKLGKDGVIGSDGERRERLPGHRVQAVDATGAGDCFAGALLARMAAGESFP
                     VALRYANAAAALTTTGYGAVAPLPRPEDVRRLLG"
     gene            complement(1142..1831)
                     /locus_tag="VD791_01930"
     CDS             complement(1142..1831)
                     /locus_tag="VD791_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HXV06760.1"
                     /translation="MSGVEASSAAGSARDVADRLESMRVIPVLRLPSREHAGAAIDCL
                     IEAGYRTVELTLTTPDAIGLLAELRRRAGADFLVGAGTVLDLDGAAACLDAGADYLVS
                     PCIVPGMARRAGERGRAALMGGFTPGEVLAAWREGAQIVKLFPAATGGPGHLAAIHAV
                     YPDIPLCPTGGVSQANLLDWFKAGARVVGVGNNVIDQKALAAGDRAQVIRHARAFLEL
                     AAGAPIEQPRA"
     gene            complement(1842..2534)
                     /locus_tag="VD791_01935"
     CDS             complement(1842..2534)
                     /locus_tag="VD791_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXV06761.1"
                     /translation="MSPDLTAALQRIWIFEGLAGEELARLAALARTRVYKAREAIVEK
                     GDPPRELFVLLSGRAKVLTRGAEGADAALNVMGPGQVFGEVAILDGQPRSATVVALEE
                     CEIAAVDVQAFRDFLAASPPVALKLLGVLAGRVRELTTRLEDRVFLDVPARLAKQLLW
                     LAENHGERTAAGAQIELRLSQQELGDLIGATRESVNKYISEWTRSGVLKQGRDRLEIF
                     DLDALRSVAGAS"
     gene            2624..3484
                     /locus_tag="VD791_01940"
     CDS             2624..3484
                     /locus_tag="VD791_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV06762.1"
                     /translation="MRILFLGSGSAHTVGTDNFQSNMLLVAGNGRCLLIDCGSDVRWS
                     LARQGFSHRDITDIYVSHLHADHIGGLEYVGFKTRFDPACRRPRLYLEASLAGPLWDH
                     SLRGGMGVITDGETRLEDFFEVVPLRAHEPFEWEGARLEVVPVAHVVAPRVTMHSHGL
                     LIGAGDQRVLLTTDTRFDADLLAPHYANADLIFQDCETSSYKTGIHAHYSELRGLPAA
                     VRAKMWLYDYPPGPLPDAVADGFRGFVRPGQSFELPDGGDRGTLRAERPMGRPVGRAP
                     GRAPGRAATS"
     gene            complement(3555..>4178)
                     /locus_tag="VD791_01945"
     CDS             complement(3555..>4178)
                     /locus_tag="VD791_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXV06763.1"
                     /translation="EGRGASGSAVSGADGAGGAGGRRAGEGGAGADGASGDAVGGAGS
                     DQESMVVGMVDAPGSRVRIDEEVTPQTLGGMLPLTVGVDEEGHFDFDEAVLRPDVKSV
                     LDELAVRLRDAEWDRLDILGYTDRIGTEDYNQQLSERRAWAVARYLMAQGIPVNKMRV
                     EGRGKRGSQLAQGECHDLERAELIACLQKDRRVEIEASIRKTEAKIQ"
BASE COUNT          600 a         1560 c         1414 g          604 t
ORIGIN      
        1 gggatgcgtg atgttgacgc cggcgaagcc gagcgtgcgc accgtcgcga gcagccgcgg
       61 cagatcgtcc gcgttctcgc cgccgtctgc aaggtcgatc agccgatact gcaggcgcag
      121 cccgtggtgc cgcgcctcct cctcgtgcag cgcgggcgac agcgagcgcc ggatgcccgc
      181 gccgatcagg cccaccagca cgtcgcccgc gctcaaccga gcagccgccg cacgtcctcg
      241 ggccgcggca gcggcgccac agcgccgtac ccggtggtgg tcagcgcggc tgcggcattc
      301 gcatagcgca gcgcgaccgg gaagctctcg cccgccgcca tgcgcgccag cagcgcgccg
      361 gcaaagcagt cgcccgcacc ggtggcgtcc acggcctgca cgcgatggcc gggaaggcgc
      421 tcgcgccgct cgccgtcgct gccgatcacg ccgtccttac cgagcttgag cgccaccagc
      481 cgggcgccgc ggcgatggca ccagtcgagc acggcgtccg gctggtcgag gtcgcacagc
      541 gcctgggctt cgtcgatgct caacagaaaa aggtccgaca tgccgagcgc ggcggtgatc
      601 acggcgcgcg cgcgtgccgc cggccacagc cgcggccgca ggttggcgtc gtaggccacc
      661 cgcacccccg cctggcgcgc cgcctccatc gccgcgaaag ccgtgtcgca ggcgctggcg
      721 ctgatcgcct gcgtgatgcc cgaggtgtgc aggaactgcg cgctgcggat caggctcagc
      781 ggcagatgct ccggccgcat gcggctcgcc gcagagccgg cgcgcagata gctgaagaca
      841 tgcccggcgg gcccgtgcgt gatgaagtac acggcagtgt gcgcgtccgg gtcgatgccc
      901 acgcccgaca cgtccaggcc ttcctctttc cacagcgcca ggaactgctc gccgaactcg
      961 tcctggccca ggcgcgtgag gtaggccacc ttcgcaccct ggcgcgccgc cgcgatggcg
     1021 cagttcgagg tgtcgccgcc gaagccctgc agccaatgat gatcctggcc ggggcgatcc
     1081 tggccgggca gcgcactgaa ctcgtacagc ggctcgccca gggaaacgat atccactgcg
     1141 ctcatgcgcg cggctgctcg atcggcgcgc ccgcggccag ctcgagaaag gcgcgcgcgt
     1201 ggcggatcac ctgcgcgcgg tcaccagcgg cgagcgcttt ctggtcgatc acgttgttgc
     1261 ccacccccac cacgcgcgcg ccggccttga accagtcgag caggttggcc tgggacaccc
     1321 cgccggtcgg acacagcggg atgtccgggt agacggcgtg aatcgcggcg aggtggccgg
     1381 ggccgccggt ggcggcggga aacagcttga cgatctgggc gccctcgcgc caggcggcca
     1441 gcacttcgcc gggggtgaag ccgcccatca acgccgcgcg cccgcgctcg ccggcgcgcc
     1501 gcgccatgcc ggggacgatg cagggcgaaa cgagatagtc ggcaccggcg tccaggcagg
     1561 ccgcagcgcc gtccagatcg agcacggtgc ccgcgccgac cagaaaatcg gcgcccgcac
     1621 gacggcgcag ttccgccagc agcccgatcg cgtcgggcgt ggtgagggtg agctccacgg
     1681 ttcgatagcc ggcttcgatc aggcagtcga tcgccgcgcc ggcgtgttca cggctgggga
     1741 ggcgcagcac cgggatcacc cgcatcgatt ccagccggtc ggccacgtcc cgggcgctcc
     1801 cggccgccga actcgcttcc acgccgctca tgccggctcg ctcaggacgc gccggcgacg
     1861 ctgcgcaagg cgtcgaggtc gaagatctcc agccggtcgc ggccctgctt gagcacgccg
     1921 ctgcgcgtcc attcgctgat gtacttgttc acgctctcgc gggtggcgcc gatcagatcg
     1981 cccagctcct gctgggacag gcgcagctcg atctgtgcgc cggcggccgt gcgctcgccg
     2041 tggttctcgg ccagccatag taattgcttg gccaggcgcg cggggacgtc gaggaagacg
     2101 cggtcttcca gccgcgtggt cagctcgcgc acgcgacccg ccagcacgcc gagcagcttg
     2161 agcgccaccg gcggcgaggc cgccaggaaa tcgcggaaag cctgcacgtc caccgccgcg
     2221 atctcgcatt cctccagcgc gaccaccgtg gcggagcgcg gctgcccgtc caggatggcg
     2281 acttcgccga acacctgccc cggccccatg acgttgagcg cggcgtccgc cccctcggcg
     2341 ccgcgcgtca gcaccttggc ccgaccgctc agcagcacga acagctcgcg cggcgggtcg
     2401 cccttctcga cgatggcttc gcgcgccttg tacacccggg tgcgggcgag cgcggccagg
     2461 cgcgccagtt cctccccggc cagaccttcg aatatccaga tgcgctgcag cgccgcggtc
     2521 aggtcggggc tcatcgggcg gcccgggtga atatgagatg tttcacaacg caggtgtgaa
     2581 gcagtttatt gggcgaggat agcactcccc gtacactgcg ggcatgcgca ttctcttcct
     2641 gggctccggg tccgcccaca ccgtagggac cgacaacttc cagtcgaaca tgctcctggt
     2701 cgccggcaac gggcggtgcc tgctcatcga ctgcggatcc gacgtccgct ggtccctggc
     2761 gcgccagggt ttctcgcacc gggacatcac cgacatctac gtcagccatc tgcacgccga
     2821 ccacatcggc ggcctcgagt acgtcggctt caagacccgc ttcgatccgg cgtgccgccg
     2881 tccgcggctg tacctggagg cctcgctcgc cgggccgctc tgggaccact cgctgcgcgg
     2941 gggcatgggg gtcatcaccg acggcgagac gcgcctggag gatttcttcg aggtggtccc
     3001 gctgcgtgcc cacgagccgt tcgagtggga gggtgcgcgc ctggaggtgg tgcccgtagc
     3061 gcacgtggtc gcaccgcggg tcacgatgca cagccacggg ctgctgatcg gcgccggaga
     3121 ccaacgcgtc ctgctcacca ccgacacgcg cttcgacgcc gatctcctgg cgccccacta
     3181 cgcgaacgcc gacctcatct tccaggactg cgagaccagc agctacaaga ccggcatcca
     3241 cgcgcattac agcgaattgc gcgggctgcc ggccgcggtg cgcgccaaga tgtggctgta
     3301 cgactatccg cccggccccc tgcccgatgc ggtggccgac ggcttccggg gtttcgtgcg
     3361 tccgggtcag tccttcgagt tgcccgacgg cggcgaccgt ggaaccctgc gcgccgagcg
     3421 tcccatgggg cgtcccgtgg ggcgtgcccc ggggcgtgcg cccgggcggg cagccacgag
     3481 ctgaacatcg gtcggtatca atgacgaagg gcccatccgg gcccttcgtc gcatggccaa
     3541 ccgagcaccg ccggttactg gatcttcgcc tcggtcttgc ggatcgacgc ctcgatctcg
     3601 acgcggcggt ccttctgcag gcaggcgatc agctcggcgc gctccaggtc gtgacactcg
     3661 ccttgggcga gctgcgaacc acgtttgcca cggccctcga cccgcatctt gttgaccggg
     3721 atgccctgcg ccatcagata gcgcgccacg gcccaggcgc ggcgctcgga cagctgctgg
     3781 ttgtagtcct cggtgccgat gcgatcggtg taacccaaga tgtcgagccg gtcccattcg
     3841 gcgtcgcgca gccggaccgc cagctcgtcc agcaccgatt tgacgtccgg acgcagcacc
     3901 gcctcatcga agtcgaaatg gccttcctcg tccacgccca ctgtcagcgg cagcatgccg
     3961 cccagggtct gcggggtgac ctcctcgtcg atgcgaaccc ggctcccggg cgcgtccacc
     4021 ataccgacta ccatcgactc ctgatccgag ccggcgccgc cgactgcatc cccgctggcg
     4081 ccgtctgcgc cggccccgcc ttcaccggca cggcggccac cggccccgcc cgctccatcg
     4141 gcgcccgaca ccgcgcttcc ggaggcaccc cggccctc
//
LOCUS       DASPGE010000062         4036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1191364, whole genome shotgun sequence.
ACCESSION   DASPGE010000062 DASPGE010000000
VERSION     DASPGE010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191364"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VD791_01950"
     CDS             <1..197
                     /locus_tag="VD791_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06764.1"
                     /translation="EPFVLAILFGANLCYVTPMAYQTNLLVMSAAGYRFADFVRGGLP
                     LTLIMWVLLSLLVPRFFPFS"
     gene            complement(249..1343)
                     /locus_tag="VD791_01955"
     CDS             complement(249..1343)
                     /locus_tag="VD791_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HXV06765.1"
                     /translation="MSPETDPQRTRRLARLIRWRLRRSRQRARVRLLAPRPHVSATRT
                     IALRAGLVLLLFALAMLVFWLDRDGLRDAADGVISFLDVVYFTMVTVTTVGYGDIVPV
                     GGRARIIDALFVTPIRIFIWFIFLGTAYQMVVQRWFEERRLRKFGQSLKDHIVLCGFG
                     HSGRIAALELVEKGHPPQELVVIDISEAAAQSAAEAGFLALVGDATTEEIQRQAAISA
                     ARAVIVCVRRDDTAVLTVLTARELAPGVRIIASCREAENIRLLRRGGADVIVSGARVG
                     GYLMADAVDSAYTVALINDLLTERGRVRLNERNPRPDEVGKSPRALACLVIGVERSGK
                     RVGFWDADELRIQADDLLLVIETTPPAQRG"
     gene            1547..2041
                     /locus_tag="VD791_01960"
     CDS             1547..2041
                     /locus_tag="VD791_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06766.1"
                     /translation="MRRQLPMTVTLATALAAAATCVLADVQSRSSEAIERAVGRASGD
                     AYWTHRSDQGVPGPLRDAYGTTIEYLSQPATEPAPEPYGRAGGYLEFAPYDAQESMLG
                     VEPMSIAFGYERRYGRVGGTIGLPGYQARLGTPDLRPVNTGALADGYWYGRAGGEIPP
                     YSLE"
     gene            2055..2360
                     /locus_tag="VD791_01965"
     CDS             2055..2360
                     /locus_tag="VD791_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06767.1"
                     /translation="MKLGREALLRDMQQIVERTQELLEAGGEKLGKTREAVAEHLEAA
                     KGSLLDLERDLERGARRAVRRADHYAQDNPWRIAGAALFLGLLLGTVLGIGAGPRRD"
     gene            2532..3890
                     /locus_tag="VD791_01970"
     CDS             2532..3890
                     /locus_tag="VD791_01970"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HXV06768.1"
                     /translation="MTWEQLPLVRTIERAVWATPLESLPSWRASLLRLTRLLVTLGRD
                     LLSGQLTLRAMSLVYTTLLSIVPLLALSFSVLKAFGVHNQIEPALRNFLAPLGSQGEE
                     IIEHIIQFVARVHVGVLGSVGFALLLYTAISLVQKIEESFNFIWHIAESRSMGERFGR
                     YLSVLLVGPILVFSALGMTAAVASSDPIRGLLAVEPLGRAAYVAGRVLPYALVIGAFT
                     FIYVFVPNTRVRIWPALTGGIVGGLLWQTGGWAFAAFVAVSTRYSAIYSGFAILVLFM
                     IWLYLSWLILLFGAAVAFYRQHPEYVLPQSGEPRLSNRMRERLALTVMSLIAQHHRDG
                     RPAWTLPQLAQRLSVPTHAVHVVLAALRQSGILAQASGDPPAYLPARDLARLSVSSLL
                     QAVRSAGEDRYLNPTSLPVPPAVEQVLQRIEEGLGVLVEDLSVSALAGPPEKSERGDP
                     AL"
BASE COUNT          598 a         1370 c         1412 g          656 t
ORIGIN      
        1 cggagccgtt cgtgctggcg atcctgttcg gcgccaacct ctgctacgtc acgccgatgg
       61 cgtatcagac caacctgctg gtgatgagcg cggcgggcta ccggttcgcc gacttcgtgc
      121 gcggcgggct gccgctgacg ctcatcatgt gggtgttgct gtcgctgctc gttccgcgct
      181 tcttcccgtt ctcctgagcc cgggcgccgc gcaaacgacg cggcgggggc cgtaccggtc
      241 gcgcccgctc aaccgcgctg cgccggcggc gtggtctcga tgaccagcag cagatcgtcg
      301 gcctggatcc gcaactcgtc ggcgtcccag aagccgacgc gcttgccgga acgctcaact
      361 ccgatcacca ggcaggcgag cgcgcgcggg ctcttgccga cctcgtccgg acgaggattg
      421 cgctcgttca agcgcacccg cccgcgttcg gtcaacaggt cgttgatcag cgcgacggtg
      481 taggcgctgt ccaccgcgtc ggccatgaga tagccgccca cgcgcgctcc ggatacgatc
      541 acatccgccc cgccgcggcg cagcagcctg atgttctccg cctcgcggca gctggcgatg
      601 atgcgcactc cgggcgcgag ctcgcgcgcg gtgagcacgg tgagcacggc ggtgtcgtcg
      661 cgccgcacgc atacgatgac cgcacgggcc gcggagatcg cggcctggcg ctggatctcc
      721 tcggtcgtgg cgtcgcccac cagggcgaga aagcccgcct ccgcggccga ctgcgcggcg
      781 gcctcggaga tgtcgatcac caccagctcc tgcggcgggt gccctttctc caccagctcc
      841 agcgcggcga tacgcccgct gtgaccgaag ccgcagagca cgatgtggtc cttcagcgat
      901 tgcccgaact tgcgcaagcg acgctcctcg aaccatctct gcaccaccat ctggtaggcg
      961 gtgccgagga agatgaacca gatgaagatg cgtatcggcg taacgaacag cgcgtcgatg
     1021 atgcgggcgc ggccgcccac ggggacgatg tcgccgtagc ccaccgtcgt gaccgtgacc
     1081 atggtgaaat atactacgtc gaggaacgag atcacaccgt cggccgcgtc gcgcagcccg
     1141 tcgcggtcga gccagaacac cagcatcgcc agtgcgaaca gcagcagtac cagcccggcg
     1201 cgcagggcga tggtgcgggt ggcactgacg tgcgggcgtg gagcaagcag ccgcacccgg
     1261 gcgcgctgcc ggctgcggcg caggcgccag cggatcagcc gggcaaggcg gcgcgtgcgc
     1321 tgcgggtccg tctcggggga catcggtata gtccggtcgc gggagtccag gcaattgtgg
     1381 cgcggatcgc ttcaacgagc agtgggacga gcaagacggc acggccgtcg agccaggcga
     1441 gtacggggaa ctcggcgccc gtgcagctgt cgctcagtag gtgccgggtt ttccgtgcac
     1501 agcgcgcgcc gtgcacgcgc gctcaccaca gcagaggaga tcaaccatgc gacgacaact
     1561 tcccatgaca gtgacgctgg cgactgccct cgcggcggcc gcgacttgcg ttctcgcaga
     1621 cgttcaatcg cgctcgtcgg aggccatcga gcgcgctgtc gggcgcgcga gcggcgacgc
     1681 ttactggacg catcgctccg atcagggcgt gccggggccg cttcgggacg cctacggtac
     1741 caccatcgag tatctctcgc agcctgcgac cgagcccgcg cccgagcctt acggccgcgc
     1801 cggcggctac ctcgagttcg ctccgtacga cgcacaggaa agtatgctcg gagtcgaacc
     1861 gatgagcatc gcgttcggtt acgagcgtcg ctacggccgc gtgggcggta ctatcggcct
     1921 gccgggctac caggcccggc ttggtacgcc ggaccttcgg ccggtgaaca ccggcgcgtt
     1981 ggccgatgga tactggtacg gccgtgccgg cggcgagata ccgccctaca gcctggagta
     2041 ggagaccgaa ggatatgaaa ctcggcaggg aagccttgct gcgggatatg cagcagatcg
     2101 tagagcgcac gcaggagctg ctggaggcgg gcggcgagaa gctcggcaag acgcgggaag
     2161 ccgtggccga gcacctcgag gcggccaagg gcagccttct cgacttggag cgtgatctgg
     2221 agcgcggggc gcgccgcgcc gtgcggcgcg cggatcacta tgcgcaggac aacccgtggc
     2281 gcatagcggg cgcggccctg ttcctggggt tgctcctcgg cacggtgctc ggcataggcg
     2341 ccggtcccag gcgcgattga ccgattcgcg ccgcgtgcgg ctgtgctgcg ccctgcgcgt
     2401 cagcgtggca gacgcgcgcg gggcgagatc gccgcaccgg tgacccccag gggcatagcc
     2461 gtgggtcagg ctcgcgaggt acgctgcgca cgccggtgcg gcggccgcgg ggaggcgcgg
     2521 gccccacggc catgacctgg gagcagctgc cgctggtgcg caccatcgag cgcgccgtct
     2581 gggcgacgcc gctcgaatcc ctgccttcct ggcgggcctc cctgctgcgc cttacgcgcc
     2641 tgctggtgac gctgggccgg gatctgctat cgggccagct taccctgcgc gccatgagcc
     2701 tggtctacac cacgctgctg tcgatcgtgc cgctgttggc gctgagtttc tccgtgctga
     2761 aggccttcgg cgtccacaac cagatcgagc cggcgctgcg caacttcctt gccccgctgg
     2821 gcagccaagg cgaagagatc atcgagcaca tcatccagtt cgtcgcgcga gtgcacgtcg
     2881 gcgtgctggg ctcggtgggc ttcgcgctgc tgctgtacac ggcgatatcg ctggtgcaga
     2941 agatcgagga gtcgttcaac ttcatctggc acatcgccga gtcgcgcagc atgggcgagc
     3001 gcttcggccg ctatctgagc gtgcttctgg tggggccgat actggtgttc tccgcgctcg
     3061 gcatgaccgc cgcggtggcg agctcggatc cgattcgagg gctgctggcg gtggagccgc
     3121 taggacgcgc cgcgtatgtc gccggcaggg tcctgccgta tgcgctggtg atcggcgcgt
     3181 tcaccttcat ctacgtattc gtccccaaca ctcgcgtgcg catctggccg gctctcaccg
     3241 gcggaatcgt cggcggattg ctgtggcaaa ccgggggctg ggccttcgcc gcgttcgtgg
     3301 ccgtgtccac ccgctactcg gcgatctact ccggcttcgc gatcctggtg ctgttcatga
     3361 tctggctcta cctgagctgg ctgatcctgt tgttcggcgc cgcggtcgct ttctatcgcc
     3421 agcatcccga gtacgtgctg ccgcaaagcg gcgagccgcg actgagcaac cgcatgcgtg
     3481 agcgcctggc gctgacggtg atgagcttga tcgcacagca tcaccgcgac ggacgccccg
     3541 cgtggaccct gccgcagctc gctcagcgcc tgagcgtgcc gacccatgcc gtgcacgtcg
     3601 tgctcgccgc gctccgtcaa tcgggcatcc tggcgcaggc cagtggcgat ccgcccgcct
     3661 atttgccggc ccgggacctc gctcggctgt cggtgagcag cctgctgcag gcggtgcgca
     3721 gcgcgggcga ggaccgctac ctcaacccga cctccctgcc ggtgccgccg gccgtcgagc
     3781 aggtcttgca acgcatcgag gaggggctgg gtgtcttggt cgaagacctc agcgtctcgg
     3841 cactcgccgg tccgcccgag aagagcgagc gcggggaccc ggcgctctga ccgagcgttg
     3901 ccggggagga gccgccggca tgcaccagcg acaccgggcg agcggcactt cccggtgacg
     3961 aaatggcatc gtttgcggca acgcgtgagc gggcgtacgc ttgctactgc gctcgaggag
     4021 tcatccatgc atacgg
//
LOCUS       DASPGE010000063         7965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_20860, whole genome shotgun sequence.
ACCESSION   DASPGE010000063 DASPGE010000000
VERSION     DASPGE010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20860"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /gene="hemA"
                     /locus_tag="VD791_01975"
     CDS             complement(<1..961)
                     /gene="hemA"
                     /locus_tag="VD791_01975"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HXV06769.1"
                     /translation="MEMQLFAFGINHQSAPLEVRERVAFNAEGLPGALRDLVDHEPVR
                     EAAIISTCNRTEVYCNAQDPGKAVQWLADYRQVRPAALEPFLYTLPRERAVKHAFRVA
                     SGLDSMVLGEPQILGQMKQAVRSAEQAGTLGLVLHKLFQQTFSVAKEVRTRTDIGAAS
                     VSMAAATVRLAERIFPGIGGQRILFIGAGEMIELTAAHFAAQHPRGMTFANRTVERAQ
                     VLATRFGAQAVSLNELSEVIAVHDIVVTSTASPLPIIGKGMVERALRRRRHRPMLMVD
                     LAVPRDVESEVAAMDDVFLYSVDDLGKVAQEGRDQRAAAVLKAE"
     gene            complement(1043..2242)
                     /locus_tag="VD791_01980"
     CDS             complement(1043..2242)
                     /locus_tag="VD791_01980"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXV06770.1"
                     /translation="MSFALRRESLPQTILAALPFLLFFPHGIVKAGIVVFALSVMVSG
                     DYRNKWRTAKASPLFWPVVGMFGISCLLAVFTDRSAPKFWSGFAHYQVYFYLLLFISI
                     GPGDWQRRAANAFFAGALLASTLFYLNLLNLLPDVRLFGDYIAYSGNNSILLGVLLAV
                     AAAGVFYEMIDHTDRRRLLWQIPAFLYIAAALLVLARTRSGSLIFIVLCLFVLLKQAA
                     RSWHRALGIAAVLALMVAATWHLSSSLQTRALKTVQDIKAFSQGEKTSGEGIRAEMYW
                     ITAQMIAEKPLAGHGIGAWLPVYQQRAKGLATGRMTTPHNDYLLYTAEIGLIGLAALF
                     WIWTTQLVVAWRIGGVHGAWVAMLSLTLIVGAMFNAILRDSVFGMSFMILMAIPLAGV
                     TRDGRAA"
     gene            complement(2306..3214)
                     /locus_tag="VD791_01985"
     CDS             complement(2306..3214)
                     /locus_tag="VD791_01985"
                     /inference="COORDINATES: protein motif:HMM:NF006487.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXV06771.1"
                     /translation="MKDALRRAGLSLALALLRLLGRLPLRWYQALGALLGHLAYHLSG
                     RYSRRMRSNLRASGLCKDEDDYRRILRANITETGKQGIEFLALWFRPQAQAAALVRSC
                     AGEQEVLDAYRAGRGVILLTPHLGCFEVSAIYAAQRFPITVLYRRPRVAWLETLIVAG
                     RGRGDEKLAPADVKGVRALLKALKSGEAVGVLPDQAPRQGEGVWVDFFGRPAYTMTLI
                     GRLWEATRPAVFVAVALRRPRGEGYDIEVRRVEGDLSGEAGARRINAAVEEAIRRCPE
                     QYLWSYSRHKRPRGAPPPPAARPHSA"
     gene            3516..4559
                     /locus_tag="VD791_01990"
     CDS             3516..4559
                     /locus_tag="VD791_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXV06772.1"
                     /translation="MKRVLVVRTSAIGDVVFATPLAAALRRTFPSAHIAWLVEQGIEG
                     LLAGDPAIDQVIVWPKTEWAQLWRTHRLGALLRAVREFRARLRAERFDTALDLQGLLK
                     SAVLTFLSGAPRRIGLDSREGGRLLLTQSLPRGGPVERISSEYLHFARLLGLDTGEFI
                     PRLAVAAAAEAQAQALLGAEGLAPGRYAVFAPFTTRPQKHWFEDAWRELAGLIQRSLA
                     ITPVLLGAPADRQAAARIAGAGSMADLTGRTSLPQAAAVIRQAALVIGVDTGLTHIGI
                     ALNRPTIALFGSTRPYLDACRPNARVIWLGLDCSPCRRHPTCNGAFTCLRNISPARVL
                     DEARLALAAERAG"
     gene            4556..5647
                     /locus_tag="VD791_01995"
     CDS             4556..5647
                     /locus_tag="VD791_01995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV06773.1"
                     /translation="MKILHVESGRHLYGGARQVLYIVEGLAARGVDNMLVCPVGSEIA
                     HAAGHSARVMPLTMRGELDAGLALRVRRLILQSRPDLVHLHSRRGADVWGAVAARLAA
                     TPCILSRRVDNPEPRWLVAVKYRLYDHVIAISEGIRQVLIAEGLDPRMVTCVRSAVDA
                     APYLSAVDRAAMCREFRLPPDALIVGMVAQLIPRKGHRHLLAALPALRAQFPRLRVLL
                     FGQGPLAPALRAEVVARGLQEVVRLLGFRDDLARWLGGLDLLVHPADMEGLGVALLQA
                     AAAAVPVIATRAGGLSEAVADGVTGVLVRPGDVADLTAAMRRLLAEPALRKRMGEAGR
                     ARVLSEFSVDAMVEGNLAVYRRVRAARAA"
     gene            5760..7433
                     /locus_tag="VD791_02000"
     CDS             5760..7433
                     /locus_tag="VD791_02000"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV06774.1"
                     /translation="MAFDLMIRHGLVVDGTGAPARHADIAIRDGRISQIGRVSGTAKA
                     SIDAADCVVAPGFIDPHTHYDAQICWDGALTPSCWHGVTSVVLGNCGVGIAPCRPGTR
                     EVAMRDLVNVEAIPYEVLERGIAWDWETFPEYLDAAQRRKPVLNLAFLAPLTPLRHYV
                     MGEASMERAATAEETVRIAALLAGAMDAGAFGFSSTILNQHLGFRGRPLACRMASRDE
                     LKAYANVLRDKAKGAIEVALTRQIGVLEEDQCEMLDLLLEQSGRPVTFIALFDRDDLP
                     EAVRDTLRRAAPLIARGARPQTSPLPLTREVDMRSPFSFAAFPSWKRVFADKRKEAQK
                     AVYRDPAFRNQFREELKNPMTFGNWERITLHEVRSAAMKRFEGCSVAEIAAAQGKDGV
                     DAFLDLTLADDLELEFTMTSFNTRVDRMSELLNNPSVLIGLGDGGAHVDMLCDAGYPT
                     YLLGTWVRERGVLGLEEAVRRLTSDPADLFGIRDRGRLLPGLAADVTIFDPASVGSSN
                     RGERRYDLPGGGKRMVMPSRGVRYTIVNGAVTYAEGALTGAAQGRVLRS"
     gene            complement(7469..7544)
                     /locus_tag="VD791_02005"
     tRNA            complement(7469..7544)
                     /locus_tag="VD791_02005"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7509..7511),aa:Phe,seq:gaa)
     gene            7732..>7965
                     /locus_tag="VD791_02010"
     CDS             7732..>7965
                     /locus_tag="VD791_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV06775.1"
                     /translation="MDLIGKTAFVTGGSGDIGGTVARALAAAGADVAISYVGHREGAS
                     ATVQAVQAAGRRGLAVQLDQRGPAAIDASVGAVV"
BASE COUNT         1259 a         2682 c         2740 g         1284 t
ORIGIN      
        1 cctcggcctt caacaccgcg gccgcgcgct ggtcgcggcc ttcctgcgcc accttgccga
       61 ggtcgtccac cgagtacagg aacacgtcgt ccatcgcagc gacctcgctc tcgacgtcgc
      121 gcggcacggc caggtccacc atcagcatcg ggcgatgccg gcggcgcctg agcgcgcgct
      181 cgaccatgcc cttgccgatg atcggcaacg ggctcgccgt gctggtgacc acgatgtcgt
      241 gcaccgcgat cacctccgac agctcgttca gggacaccgc ctgcgcgccg aagcgggttg
      301 ccagcacctg cgcccgctcg accgtgcggt tggcgaaggt catcccgcgc ggatgctgcg
      361 cggcgaagtg cgcggccgtc agctcgatca tctcgccggc accgatgaac aggatcctct
      421 gcccgccgat gccggggaag atgcgctcgg cgagccgcac cgtggcggcg gccatggaca
      481 ccgaagcggc gccgatgtcg gtgcgcgtgc gcacctcctt ggccaccgag aaggtctgct
      541 ggaacagctt gtgcagcacc agcccgagcg tgcccgcctg ctcggcgctg cgcaccgcct
      601 gcttcatctg ccccaggatc tgcggctcgc ccagcaccat ggagtccaac ccgctcgcca
      661 cgcggaatgc gtgcttcacc gcgcgctcgc gcggcagggt gtacagaaac ggctcgagcg
      721 cggccggccg cacctgccgg tagtccgcca gccattgcac cgccttgccc gggtcctggg
      781 cattgcagta gacctcggtg cggttacagg tggagatgat cgccgcctcg cgcaccggct
      841 cgtggtcgac caggtcgcgt agcgcccccg gcaggccctc ggcgttgaag gcgacccgct
      901 cgcgcacctc cagcggggcg ctctgatggt tgatcccgaa ggcgaagagc tgcatctcga
      961 ttgccgccgg tgggagctgt tctcccccgg gaccggcatt atagccagta gccgggatct
     1021 agtcgctatg tacgcttgtt cgtcatgcgg cccgcccgtc gcgcgttacg ccggccagcg
     1081 gaatggccat caagatcatg aacgacatgc caaacaccga gtcgcgcaag atggcgttga
     1141 acatggcccc gacaatcaag gtcagcgaca gcatggccac ccaggcaccg tggacgccgc
     1201 cgatccgcca cgcaacgacc agttgcgtgg tccagatcca aaacagggcg gccagcccga
     1261 tcaacccgat ttcggccgta tacaacaggt agtcgttgtg cggggtggtc atccttccgg
     1321 tggccagccc tttcgcgcgc tgttgataca ccggcagcca ggcgccgatg ccatggccgg
     1381 ccagcggctt ctcggcgatc atctgcgcgg taatccagta catctccgcg cgaatcccct
     1441 cgccgctggt tttctctccc tgcgaaaagg ccttgatgtc ttgcaccgtc ttcagtgcgc
     1501 gcgtctgcag gctcgatgaa agatgccatg tggcggcgac catcaaggcc agcacggccg
     1561 ctatcccgag tgcacggtgc caggaccgcg cagcctgttt cagaagcacg aacagacaga
     1621 gtacgatgaa gatcaggctt ccgctgcgag tccgcgccag gaccagcagc gcggcggcga
     1681 tgtacaggaa tgccgggatt tgccacaaca accgtcggcg gtcggtgtgg tcgatcatct
     1741 cgtagaacac gccggcggcg gcaactgcca gcaagacgcc gagcaagatc gaattgttcc
     1801 ccgaataggc aatgtagtcg ccgaaaagcc ggacgtcggg aagcagattg agcaggttga
     1861 ggtaaaacag cgtcgaggca agcagcgcgc cggcgaaaaa agcgttggcc gcccttcgct
     1921 gccaatcgcc tggtccgatg ctgatgaaaa gcagcaggta aaagtacacc tggtagtgcg
     1981 caaagccgga ccagaacttg ggcgcggacc tgtccgtaaa gacagccagc aaacaactga
     2041 tgccaaacat gccaacaacc ggccagaaca gcggactcgc tttggcggtg cgccatttgt
     2101 tccggtagtc gcctgatacc ataacgctca gcgcgaaaac aacgatcccg gccttcacga
     2161 tgccgtgcgg gaaaaacagc agaaacggca atgcagcgag tatcgtttgc ggcaagctct
     2221 cgcgcctgag cgcgaagctc atgtttctta tcatttccgc cccggtctgt tgcataaagg
     2281 acgccattga ctggtcggac ctttctcagg cgctgtgagg ccgcgcggcc ggcggtggcg
     2341 gcgcgccgcg cggacgcttg tggcggctgt aggaccagag gtactgctcc gggcaacggc
     2401 ggatcgcctc ctcgactgcg gcgttaatgc ggcgcgcccc ggcttcgccc gacagatcgc
     2461 cctccacgcg ccgcacttcg atgtcatagc cttctccccg cgggcggcgc agcgcgacgg
     2521 caacgaacac cgccggccgc gtggcttccc agagcctgcc gatcagcgtc atggtgtacg
     2581 cgggacggcc gaagaaatcc acccagaccc cttcgccctg gcgcggcgcc tggtccggca
     2641 gcacgccgac ggcctcgccg ctcttgagcg ccttgagcag agcgcgcacg cctttcacgt
     2701 cggcgggtgc cagcttttcg tctccacggc cgcgaccggc gacgatcaga gtctcgagcc
     2761 aggcgacgcg cggccggcgg tacaggacgg tgatcggaaa cctctgggcg gcgtagatcg
     2821 ccgacacttc gaaacagccc agatgcgggg tgagcaggat cacgccccgt cccgcacgat
     2881 aggcatcgag cacctcctgc tcgccggcgc acgagcgcac cagcgcggcg gcctgcgcct
     2941 gcgggcgaaa ccagagcgcg agaaattcga tgccctgctt gccggtctct gtgatgttcg
     3001 cgcgcaggat ccggcggtag tcgtcttcgt ctttgcacag gccgctggcg cgcaggttgg
     3061 aacgcatgcg ccgcgagtag cggccggaca ggtgataggc caggtgtccg agcaaagcgc
     3121 ccagcgcctg ataccaacgc agcggcagcc ggccgagcag gcggagcagc gcaagggcca
     3181 gcgacaggcc cgcgcggcgc agagcgtcct tcacgcgggc cggatcgccg atgctgcgac
     3241 gagatccacg ggcaccggga cggcgggtgc acgctgttgc ggtcgatcga tcgctccaat
     3301 ccctctcatc tcgaacaccg gcgcggtgcc gttgtgtgac gggtccacat ccggccactg
     3361 tcgccgggga gcttgcccgc gccgtactgt agaatatctg cacgccaatt gcacgcgtcc
     3421 atcgcgtccg aaatgccagg cacggttgcc gtctgcttcg tcgcttcgcc gctgctgcgc
     3481 ggggacaccg gcacctggca caagctcgcc gggcgatgaa gcgcgtcctg gtggtgcgca
     3541 cttcggcgat cggcgacgtc gtgttcgcca cgccgctggc tgcagcgctg cgacgcacct
     3601 ttccatccgc gcacatcgcc tggctggtgg agcaggggat cgaaggtctg ctcgccggcg
     3661 acccggcgat cgaccaggtg atcgtatggc ccaagaccga atgggcgcag ctgtggcgaa
     3721 cgcatcggct gggcgcgttg ttgcgggcgg tgcgcgagtt ccgtgcgcgc ctgcgcgccg
     3781 agcgcttcga cacggcgctc gacctgcagg gcctgttgaa gagcgcggtg ttgacgttcc
     3841 tgtccggtgc tccccgccgc atcggcctgg actcccggga aggcggccgg ctgctcctga
     3901 cgcaaagtct tccgcgcggc ggtccggtcg agcgcatcag ctccgagtac ctgcacttcg
     3961 cgcggctgct cggactggat accggcgagt tcatcccgag gctcgccgtc gccgctgcgg
     4021 ccgaggcgca ggcgcaggcg ctgctcggcg ccgagggcct tgcaccgggc cggtacgcgg
     4081 tgttcgcgcc gttcaccacc cgtccgcaaa agcactggtt cgaggacgcg tggcgcgagc
     4141 tggccggcct gatccagcgc tcgctcgcga tcacccccgt gctgctcggt gcgccggcgg
     4201 atcgccaggc ggccgctcgc atcgccggcg cggggtcgat ggcggacttg accgggcgca
     4261 cgtcgctgcc gcaggccgcg gccgtcattc ggcaggccgc gctagtgatc ggcgtggaca
     4321 cgggattgac gcacatcggc attgcgctga accggccgac catcgccctg ttcggatcga
     4381 cgcggcccta tctggatgcc tgtcgcccca atgcccgcgt gatctggctg gggctcgact
     4441 gctccccttg tcgccgccat cccacctgca acggcgcttt cacctgcttg cgcaacatct
     4501 cgccggcacg cgtgctcgac gaagcgcgac tggcgctggc cgcagagcgt gccgggtgaa
     4561 gatcctgcac gtcgagtccg gccgccacct ctacggaggc gcccggcagg tgctgtatat
     4621 cgtcgagggg cttgctgcgc gcggtgtcga caacatgctg gtgtgcccgg tcgggagcga
     4681 gatcgcgcat gcggcgggac acagcgcccg agtcatgccg ctgacgatgc gcggcgagct
     4741 ggacgcgggc ctcgcgttgc gcgtacggcg actcatcctg cagtcgcgtc ccgacctggt
     4801 gcatctgcac agccggcgcg gagccgacgt atggggtgcg gtcgctgcac gcctcgcggc
     4861 cacaccctgc atcctctcgc gccgcgtcga caaccccgag ccgcgctggc tggtggcggt
     4921 caagtaccgg ctgtacgatc acgtcatcgc gatctcggaa ggcatccggc aagtgctgat
     4981 cgcggaaggg ctcgacccgc ggatggtgac ctgcgtgcgc agcgcggtcg atgcggcgcc
     5041 ctatctgtcc gctgtcgacc gcgccgcgat gtgccgcgag ttccggctac cgcccgatgc
     5101 gctgatcgtc ggcatggtgg cgcagctcat cccacgcaag gggcatcgcc acttgctggc
     5161 ggcgctgcct gcgttgcgcg cgcagttccc gcgcctgcgc gtgctgctgt tcggccaggg
     5221 tccgctggcg ccggcgctgc gggccgaggt cgtcgcacgg ggactgcagg aggtggtgcg
     5281 gctgctgggc ttccgtgacg acctcgcgcg ttggctcggg ggactcgatc tgctggtgca
     5341 tccggcggac atggaagggc tcggggtggc cttgctgcag gccgccgcgg cggcggtgcc
     5401 ggtgatcgcg acccgtgccg gcggattgag cgaggcggtg gccgacgggg tcacgggtgt
     5461 gctggtgagg cccggcgacg ttgccgatct gaccgcggcc atgcgccggc tgctcgccga
     5521 accggcgctg cgcaagcgca tgggtgaggc cgggcgcgcg cgcgtgctgt ccgagttctc
     5581 ggtggacgcg atggtggagg gcaatctggc cgtgtaccgg cgggttcggg cagcgcgcgc
     5641 ggcatgatcc gccctgcgca ccgatcggca ggacggttcg cggttgccgg acgcgggttc
     5701 gatgcgaagc tgcagcatgc tagagtgatg gctgagcagg gcgttggaga tacgcacgga
     5761 tggccttcga tctgatgatc cgacacgggc tggtcgttga cggcaccggc gcaccggcgc
     5821 ggcatgccga catcgcgatc agagacgggc ggatcagcca gatcggacgc gtgagcggca
     5881 ccgccaaggc gtcgatcgat gccgccgact gcgtggtcgc gcccggattc atcgacccgc
     5941 acacgcacta cgacgcgcag atttgctggg atggcgcact gaccccgtcc tgctggcatg
     6001 gggtcaccag cgttgtgctg ggcaactgcg gcgtgggaat cgcgccgtgc cggcccggca
     6061 ctcgtgaagt cgcgatgcgc gatctggtca atgtcgaggc gattccctac gaggtgctcg
     6121 agcgcggtat cgcctgggac tgggagacct ttcccgagta cctggacgcc gcgcagcgcc
     6181 gcaagcctgt cctgaatctt gccttcctgg cgccgctcac gccgctgcgg cattacgtga
     6241 tgggcgaggc gtcgatggag cgcgccgcga ccgcagagga aacggtgcgt atcgccgcgc
     6301 tgcttgccgg agcgatggac gccggggcgt tcggattctc cagcacgatc ctcaaccagc
     6361 atctcggctt ccggggccgc ccgctggcct gccgtatggc gagccgtgat gagctcaagg
     6421 cctacgcgaa cgtgctcaga gacaaggcca agggcgcgat cgaggtggcc ctgacgcggc
     6481 agatcggcgt gctggaggaa gatcagtgcg agatgctcga tctgttgctc gagcagagcg
     6541 gccggccggt gaccttcatc gcgctgttcg atcgcgacga cctgccggaa gcggtgcgcg
     6601 acacgctgcg ccgtgcagcg ccgctgatcg cacgcggcgc ccgcccgcag acctcgccgc
     6661 tgccgctcac ccgcgaagtc gacatgcgca gcccgttctc gttcgccgcc tttccgtcct
     6721 ggaagcgcgt gttcgcggac aagcgcaagg aggcgcagaa ggcggtgtac cgcgatccgg
     6781 cgttccgcaa tcagttccgc gaagagctga agaatccgat gacgttcgga aactgggagc
     6841 ggatcaccct gcacgaggtc cgctcggctg cgatgaaacg cttcgaggga tgctcggtcg
     6901 cggagatcgc cgccgcgcag ggcaaggacg gcgtcgatgc ttttctcgac ctcacactgg
     6961 ccgacgatct cgagctcgag ttcaccatga cctcgttcaa cacgcgcgtc gaccggatga
     7021 gcgagctgct caacaatccc agcgtgctga tcgggttggg cgacggcggc gcgcacgtgg
     7081 acatgctctg cgatgcgggc tatcccacct acctgctcgg cacctgggtg cgcgagcgcg
     7141 gcgtgctcgg tctggaggag gcggtgcggc gtctgacctc agacccggcc gacctgttcg
     7201 ggattcgcga ccgcgggcgc ctgctcccgg ggctcgcggc cgatgtcacc atattcgatc
     7261 cagccagcgt cggctcctcg aaccgcggtg agcggcgtta cgacctgccg ggcggcggca
     7321 agcggatggt gatgccctcc cgcggcgttc gctacaccat cgtcaatggc gcggtcactt
     7381 acgcagaggg ggcgctgacc ggcgccgcgc agggacgcgt gctgcgcagc tgacccgcac
     7441 aggcggtcta ctgcgaggag agtgaaggtg gtggccaagg gcggaatcga accaccgaca
     7501 caaggatttt cagtcctctg ctctaccaac tgagctactt ggccgtgcct gcacgatgtt
     7561 cgcggcccca agatgaactg cgcgaggccg ctctccagca gcggagttca cgccgggacg
     7621 gagtcccgcc gcgaaagccg cgcattatag ccgggccggc aggtattgtg ttccctggcc
     7681 ctgctgaaca aggatcgctt ttccggtgac cggctgcagt gagagcgcga tatggatctg
     7741 atcggcaaga ccgccttcgt cacgggcggg tctggagaca tcggaggcac cgtagcccga
     7801 gcgctggcag cggcgggtgc ggatgtcgcc atctcctacg tggggcaccg cgaaggcgca
     7861 tccgcgaccg tgcaggcggt gcaagcagcc ggccggcgcg ggctggcggt gcaactcgat
     7921 cagcgcggcc cggccgccat cgacgcctcg gtcggggccg tggtc
//
LOCUS       DASPGE010000064         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_137910, whole genome shotgun sequence.
ACCESSION   DASPGE010000064 DASPGE010000000
VERSION     DASPGE010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137910"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VD791_02015"
     CDS             <1..1059
                     /locus_tag="VD791_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXV06776.1"
                     /translation="AEGDIALVLHTSGTTSRPKIVPLTQRNLCASARNVCRTLALTAA
                     DRGLNIMPLFHIHGLVAATLAPLSAGGQVCCTPGFNALKFFGWMAEARPTWYTAVPTM
                     HQAILARAGRSAEVIRANPLRFIRSSSSSLAPQIIKELEEVFGAPVIESYGMTEAAHQ
                     MASNPLPPAQRKPGSVGIAAGPEMCVLDAAGSVLPAGATGEIAIRGENVTAGYENNSK
                     ANAEAFADGWFRTGDQGVMDAAGYFTITGRLKEIINRGGEKISPREVDEVLLDHPAVQ
                     QCVTFAVPHDKLGEDIAAAVVLREGAQADERALCEFVARHLADFKVPRKILILPELPK
                     GATGKIQRIGLAQKLGLA"
     gene            complement(1056..2294)
                     /locus_tag="VD791_02020"
     CDS             complement(1056..2294)
                     /locus_tag="VD791_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06777.1"
                     /translation="MAYDEALYSDVIITWLGAIGVWSYLDFVHRHRTGSVLERRVLFL
                     LYCLCVLLAGRGFYWLLGWSWLEYVEMLPATLIPLAVTLVAEGLIRQHVSMPMKIYVA
                     AGTAVFLLLGAAGLLGRDLPPLLLGLMAFQVSVIAAIGWILWRTDRAMLKASEIRLNN
                     AMIVAAMFAVPLVVTDFREALTWIPRRLGAAGALIFVYVLLRMTYRADTRRIVVAEVA
                     GIFLRAAVMAAAFMAITGRLTLPEFFGMFPLSVTFVLVFTIFYRLKAVSVENRGLSFL
                     QWLLHARMSGLDAFAQSLRHLPLAEEHVLLRDGDLARYDVRAIVDHLGAQRGVVSLTR
                     LRTLGHAGAAEQLIDILERHGMNHVGLVGERPPALLLLNTPLVPGAHLDEIKINLILK
                     LCRVLSSPPLQARTAAAAGY"
     gene            complement(2330..>3128)
                     /locus_tag="VD791_02025"
     CDS             complement(2330..>3128)
                     /locus_tag="VD791_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06778.1"
                     /translation="VVRTVVPEGVYYGVLTGDKHRLDNALIAILYERRSHRPIAFNAF
                     AYLEVELHGRPVDVLHLGLIMVDPERRAKGYSWLIVGLPCLLVFLRSGLQPVWITSVS
                     QVPAVCGAVARGFTGVYPSPVAESRCTFTHLSIARQVMERHRAAFGAGPQAQFDEDRF
                     IIRDGYAPGVENLKKTFERVAKHRDERVNDACRRSLDYARGDDFLQVGSIDFACTREY
                     LLRSVPRDSLPSVLYRATFVLLGRLVVPLVHWFLPRRQWGDLRPWRP"
BASE COUNT          580 a         1061 c         1053 g          434 t
ORIGIN      
        1 gcggaggggg acatcgcgct ggtgctgcac acctccggca ccacgtcgcg cccgaagatc
       61 gtaccgctca cccagcgcaa cctgtgcgcc tcggcgcgca acgtgtgccg gacgctggcg
      121 ctcaccgccg ccgaccgcgg cttgaacatc atgccgttgt tccacatcca cggcctggtc
      181 gccgccacgc tcgcgccgct ctcggccggc gggcaggtgt gctgcacgcc ggggttcaac
      241 gcgctgaaat tcttcggctg gatggccgag gcgcgtccca cgtggtacac cgccgtgccg
      301 acgatgcatc aagcgatcct cgcgcgggcc ggccggagtg cggaggtgat ccgagccaat
      361 cccctgcggt tcatccgctc ctcgtcctca tcgctcgcgc cgcagatcat caaggaactg
      421 gaggaagtgt tcggcgcgcc cgtgatcgag tcctacggca tgacggaagc cgcgcaccag
      481 atggcgagca acccgctgcc gccggcccag cgcaagcccg gctcggtcgg catagcggca
      541 ggacccgaga tgtgcgtgct ggatgccgca ggcagcgtgc taccggctgg cgccactgga
      601 gaaatcgcca ttcgcgggga gaacgtcacc gcgggctacg agaacaactc caaggcgaac
      661 gccgaagctt tcgccgacgg ctggtttcgc accggagacc agggcgtgat ggacgcagca
      721 ggctacttca cgatcaccgg ccggctaaag gagatcatca accgcggcgg cgagaagatc
      781 tcgccgcgcg aggtggacga ggtgctgctc gaccacccag cggtgcagca atgcgtgacc
      841 ttcgccgtgc cccacgacaa gctcggcgag gacatcgccg ccgcggtggt gctgcgcgaa
      901 ggggcgcagg ccgacgagcg ggcgctgtgc gaattcgtgg cgcggcacct ggccgatttc
      961 aaggtgccgc gcaagatcct gatcctgccg gagctgccca agggcgccac cggcaagatc
     1021 cagcgcatcg gactggcgca gaagctgggg ttggcctagt accctgcggc ggccgcggtg
     1081 cgtgcctgca gcggcgggct cgacagcacg cggcacagct tcaggatcag gttgatcttg
     1141 atctcgtcca gatgcgctcc gggcaccagc ggggtgttga gcagtagcag cgccggcggc
     1201 ctctcgccta ccaggcccac gtggttcatg ccgtggcgct cgagaatgtc gattagctgc
     1261 tcggccgcgc cggcgtgccc gagcgtacgt aacctggtga ggctcaccac cccgcgctgc
     1321 gcgccgaggt gatcgacgat cgcacggacg tcgtaacgcg ccaggtcgcc atcgcgcagc
     1381 aggacatgct cctcggcgag gggcaggtgc cgcagcgact gggcgaaggc atccaggccg
     1441 gacatgcgag cgtgcagcag ccattgcagg aaagacagtc cgcggttctc gacgctgacc
     1501 gctttcaacc ggtagaagat ggtgaacacc agcacgaacg tcaccgacag cgggaacatg
     1561 ccgaagaact caggcagcgt caggcgaccg gtgatggcca tgaacgccgc ggccatgacc
     1621 gcggcgcgca ggaagatgcc tgcaacctcg gccaccacga ttctccgggt gtcggcgcgg
     1681 taggtcatgc gcagcaacac gtagacgaag atcaacgccc cggccgcgcc caggcgccgc
     1741 gggatccagg tcagcgcctc gcggaagtcg gtcaccacca gcggcactgc gaacatggcc
     1801 gccacgatca tggcgttgtt gagccggatc tccgaggcct tcagcatggc gcgatccgtg
     1861 cgccacagta tccagccgat cgccgcgatc accgagacct ggaacgccat cagccccagc
     1921 agcagcgggg gcagatctct gccgagcagc ccggcggccc cgagcagcaa gaagacggcg
     1981 gtgcccgcgg cgacatagat cttcatcggc atgctcacgt gctggcgaat cagaccctcg
     2041 gcgaccagcg tgaccgcgag ggggatcaga gtggccggca gcatctccac gtactccagc
     2101 cagctccagc cgagcagcca gtagaagccg cggcccgcca gcagcacgca caggcagtac
     2161 aggaggaaca gcaccctgcg ctccagtacg ctgccggtgc ggtgacgatg gacgaagtcg
     2221 agatagctcc aaacgccgat cgccccgagc caggtgatga tgacgtcgct gtagagagcc
     2281 tcgtcgtaag ccatgttccc tcctcaccac gcaacggcgg atgtcccgac tacggccgcc
     2341 agggacgcag gtcgccccat tgccgtctcg gcaagaacca gtgcaccagc ggaacgacca
     2401 gacggccgag gagcacgaag gtcgcgcgat aaaggacgct gggcagggag tcgcgcggca
     2461 ccgacctcag cagatactcg cgcgtacagg cgaagtcgat cgatccgacc tgcaggaaat
     2521 cgtcgccgcg cgcatagtcg agcgagcggc ggcatgcgtc gttgacccgc tcgtcgcggt
     2581 gcttggcgac ccgctcgaag gtctttttca ggttctcgac cccgggagca tagccgtcgc
     2641 ggatgatgaa gcggtcctcg tcgaactgcg cctgcgggcc ggccccgaag gcggcgcggt
     2701 ggcgttccat cacctggcgg gcgatgctca ggtgagtgaa ggtgcagcgc gattccgcga
     2761 ccggcgaggg gtataccccg gtgaagcccc gggcgacggc tccgcacacc gccggcacct
     2821 ggctcacgct ggtgatccag accggctgca ggccgctgcg caggaacacc agcagacacg
     2881 gcagcccgac gatgagccac gagtatccct tcgcgcggcg ttcggggtcc accatgatca
     2941 gtcccagatg cagcacgtcc accgggcggc cgtgcagctc gacttccaga taggcgaagg
     3001 cgttgaacgc gatcggccga tgcgaccgcc gctcatagag gatcgcgatc agggcgttgt
     3061 ccagccggtg cttgtcgccg gtgagcacgc cgtaatacac gccctccgga acgacggtgc
     3121 gcaccacc
//
LOCUS       DASPGE010000065        10056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_489587, whole genome shotgun sequence.
ACCESSION   DASPGE010000065 DASPGE010000000
VERSION     DASPGE010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10056
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489587"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1018
                     /gene="fdxH"
                     /locus_tag="VD791_02030"
     CDS             158..1018
                     /gene="fdxH"
                     /locus_tag="VD791_02030"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HXV06779.1"
                     /translation="MPLQSLDITRRSATTTQPPSVREQTKVAKLIDISKCIGCKACQS
                     ACMEWNDLRDMVGDAGGAYDNPRDLTDKSWTVMRFFEVEPDGKLEWLIRKDGCMHCAD
                     PGCLKACPSPGAIVQYSNGIVDFHQEHCIGCGYCITGCPFDVPRISKKDSKAYKCTLC
                     SDRVSVGLEPACVKTCPTGAIVFGSKEDMIVHADERIADLKERGYGNAGRYDPAGVGG
                     THVMYVLQHADRPELYNLPKDPQISPLVSLWKGVGKPIALLALAAAAIGSFFHYVKVG
                     PNEVEEEETR"
     gene            1095..1715
                     /locus_tag="VD791_02035"
     CDS             1095..1715
                     /locus_tag="VD791_02035"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HXV06780.1"
                     /translation="MKSNLIERYGAEDRINHWVIAITFVLLALSGLALFHPSLFWLTN
                     LFGGGPWTRILHPFVGVVMFVAFAWLALGFWRHNVLGDNDRRWLKQWRDVMNNREDRL
                     PEVGRYNAGQKVLFWAMVVCMAILIVSGIVIWQPYFAPVFPIALIRIASLLHALAAFV
                     LILGIIVHIYAALWVKGSIGAMVRGTVTRAWARKHHPGWYREITGD"
     gene            1818..2750
                     /gene="fdhE"
                     /locus_tag="VD791_02040"
     CDS             1818..2750
                     /gene="fdhE"
                     /locus_tag="VD791_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HXV06781.1"
                     /translation="MSEIIRDGSLAGGIPTVPRFRAVGAGVFAERAARLRTLAAGHAL
                     EGYLRFAAALIDAQDKALRAATNSSSSGWSSAPDEQTLQRCREHGLPPLSVDGHRRDA
                     VWREVLAGIAGTLNPDALPPEARNVLSALAAAPVDSLESAASSLLAGAYAEVDAGWAP
                     FIAAALQVYWVRMALRLGEAGFSPDVRYGLCPVCGSHPVASVVRVGGAAQGLRYLVCS
                     LCASEWHVVRVKCAACASTKGIGYLGIEGASPAIKAECCDECRAYLKIFYLEKDTALD
                     PVADDLATLALDMLVDEQGYDRAGPNLLFLPGAV"
     gene            complement(2757..3527)
                     /locus_tag="VD791_02045"
     CDS             complement(2757..3527)
                     /locus_tag="VD791_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXV06782.1"
                     /translation="MPTLDIDAATESIWRHLQAGVHYPLEWSRRLSMVDGYRIQLGIL
                     ERLRVAGERHAGWKVGLTAAAMRAQHGVHEPCFGFLLASGNRPSGHAFRHAELIKPGF
                     ENELCLTVGSTLRGPGVTFDQARSAIAEVAPALEIVEKRGDFAADLPLTMADNAQQKA
                     FVTAAARPLGDYDLAGATVRVYVDHALIDRADGAQVMGNPINSIVWLANKLAEFGRAL
                     EAGQRVISGSFTRQYPLDQPAHVRSEFEPFGVVEASFV"
     gene            3672..5102
                     /gene="selA"
                     /locus_tag="VD791_02050"
     CDS             3672..5102
                     /gene="selA"
                     /locus_tag="VD791_02050"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HXV06783.1"
                     /translation="MNEPLPTLLSPRPGPAALPSVDRLVGLARVAQLVDAYGRDAVYA
                     AVRALLEEKRRAVLGGALPESVWPPGEIWVEADLRARLEADAALKLRPVFNLTGTVLH
                     TNLGRAPLPEEAVAAVAKAMRGAVNLEYDLASGARGERDELVEGLLTELTGAQAATVV
                     NNCAAAVLLVLNTLALRGDVVVSRGELIEIGGAFRMPDIMARAGAKLVEVGTTNRTHA
                     ADYEHAIGLRTALVLKVHPSNYSVEGFSTLVDEEELARIAHRHQLPLFVDLGSGALAD
                     LREFGLPGEPTAKALLAAGADLVAFSGDKLLGGPQAGMVVGRKELIAKLRKNPLKRAL
                     RVDKMTLAALEAVLRLYRNPDRLGRRLTTLRLLTRSQAEIVALGERLLEPVRAAFAAQ
                     ADVSLQECQSQIGSGSLPVDRLPSAALVLTPRVQGKGEGTALKALETRLRDLPVPVIG
                     RVHEGALWLDLRCLEDEAQFLAQLAT"
     gene            5099..7054
                     /gene="selB"
                     /locus_tag="VD791_02055"
     CDS             5099..7054
                     /gene="selB"
                     /locus_tag="VD791_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HXV06784.1"
                     /translation="MIVGTAGHIDHGKTALLKALTGVDADRLPEEKARGITLDLGYAY
                     TPLPGGEVLGFVDVPGHERLVHNMLAGATGIDFVLLVVAADDGPMPQTREHLQIVDLL
                     GLSHGAVAVTKIDAVSAERAAQAQDEVRRLLRGTALEGAPLFPVSSVTGGGVDALRAH
                     LGRAAGEPGGRDSRGHFRLAIDRCFTLKGTGTVVTGAVHSGTVRVGDELLLSPPGIPV
                     RVRGIHAQDRAAEAGLAGQRCALNLAGPEFDRQIVQRGHWVLEPALHAPTQRIDVQLR
                     LLDSEPRALKHWTPAHVHLGARDVPGRVALLEGETLEPGATALAQLVLDRPDAVLAGD
                     RFVVRDQSASRTMGGGVALDPFPPQKGRRASGRLSLLRTWRSGDARAAFTAALGTAAL
                     GIDVQRFALAWNLREEERPALFDGVAMRRVRSAATDLVFSASAWQGLRQALLAAVAAE
                     HGRAPDMVGVSRERLRRLGAPSLAPAAYEALLDELLGEGLIVASGAWLHDPAHRVRFG
                     AQEEHLWSKVRPMLDATPFHPPRVRDLAHALHVEEEAMRRLLQRAARLGVVYPVAHDH
                     YFTLHAVDALAEIVRDLNRAKGAALAAEFRDRIGTGRRLAIQILEFFDRVGYTRRAGD
                     AHLLRQPALFAAREAPLPLRDRGGGEG"
     gene            7063..7160
                     /locus_tag="VD791_02060"
     tRNA            7063..7160
                     /locus_tag="VD791_02060"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7098..7100,aa:Sec,seq:tca)
     gene            7190..8008
                     /gene="fdhD"
                     /locus_tag="VD791_02065"
     CDS             7190..8008
                     /gene="fdhD"
                     /locus_tag="VD791_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HXV06785.1"
                     /translation="MNDSTRPIAAFGEYAVQRWDGARGASNVDRVAEEVPVSLVYNGL
                     AHAVMMASPADLEDFALGFSLSEGIVASPAELFRVDVVPLDEGILLRMEIEPARCVPL
                     EEQRRHLAGRTGCGLCGAESIEQAVRHPARVSGGPRIAPAALHAAFARLAQSQPLSAV
                     TGGVHAAAWAGVDGELLAVREDVGRHNALDKLIGALVRGGRRMDEGFALITSRASFEM
                     AQKAATVGITLLAAISAPTGLAIRLAEETGLTLVAFARDGKHNVYANPARILVA"
     gene            complement(7997..8770)
                     /locus_tag="VD791_02070"
     CDS             complement(7997..8770)
                     /locus_tag="VD791_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXV06786.1"
                     /translation="MQWVEAAGQRLECVRLAASHARAAAPSIVFLHEGLGSVSMWRDF
                     PQRVADATGCEAVVYSRQGYGRSDPPTAPLPVRYMHDEALIVLPALLERLRIERPILL
                     GHSDGASIALIHAGSASPAPSGVIAMAPHVMVEELSVASIAQARVAYETTDLRQRLSR
                     HHADPDAAFWSWNRIWLDPAFREWNIESCLPSIACPILAIQGEDDEYGTMEQIERIAR
                     QARDVDVVKLADCGHSPHRDQPDAVLAAMEAFVRRVLGD"
     gene            complement(8772..>10056)
                     /locus_tag="VD791_02075"
     CDS             complement(8772..>10056)
                     /locus_tag="VD791_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HXV06787.1"
                     /translation="NTLLTAADYEYMLRDSRARALIVSAPLLATFAPLLPRLPFLEHV
                     IVSGGDAAGHRPFVSLLDSARGAFAPAPTTADDACFWLYSSGSTGSPKGTVHVHGSLI
                     QTAELYARPILGIEEGDLVFSAAKLFFAYGLGNALTFPMAVGATAVLMAERPTPASVF
                     ARLRKHQPTIFYGVPTLYAALLASPELPERGALRLRRCASAGEALPEEIGKRWSAHFG
                     VDILDGIGSTEMLHIFLSNRPGDVRYGTTGKPVPGYEVRLTDEYGMPVAPGEQGELQI
                     SGPTSAAFYWNNRDKTRATFQGPWTRSGDKYLQDADGYYVYAGRSDDMLKVSGMYVSP
                     VEVESALITHEAVLETAVVGREDHDKLTKPMAYVVLKPGRGATPALAAELQQHVKTRL
                     APFKYPRWVEFIDELPKTATGKIQRFKLRARLARP"
BASE COUNT         1460 a         3442 c         3630 g         1524 t
ORIGIN      
        1 gcaccaggtc ggcatcccga tccactgggg cttcaagggc gaggcgcgcc cgggctacct
       61 gacgaatacg ctgaccccgt tcgtcggcga cgccaatacg cagacgcccg agttcaagtc
      121 gttcctggtg aacatggcga aggcatagga gcagcgtatg ccacttcaat ctctcgacat
      181 cacgcgccgc tccgccacca ccacgcagcc gccctcggtg cgcgagcaga ccaaggtcgc
      241 caagctgatc gacatctcca agtgcatcgg ctgcaaggcc tgccagagcg cgtgcatgga
      301 atggaacgac ctgcgcgaca tggtgggcga cgccggcggc gcctacgaca acccgcgcga
      361 cctcaccgac aagtcctgga cggtgatgcg cttcttcgag gtggagccgg acggcaagct
      421 cgagtggctg atccgcaagg acggctgcat gcactgcgcc gatcccggct gcctcaaggc
      481 ctgtccgtcg ccgggcgcga tcgtgcagta ctccaacggc atcgtcgatt tccaccagga
      541 gcactgcatc ggctgcggct actgcatcac cggctgcccg ttcgacgttc cgcggatctc
      601 gaagaaggac agcaaggcgt acaagtgcac cctgtgctcc gaccgcgtgt cggtgggcct
      661 ggagcccgcc tgcgtcaaga cctgtcccac cggcgccatc gtgttcggct cgaaggagga
      721 catgatcgtg cacgcggacg agcgcatcgc cgacctgaag gagcgcggat acggcaacgc
      781 gggccggtac gatccggcgg gcgtgggcgg cacgcacgtg atgtacgtgc tgcagcatgc
      841 cgaccggccg gagctgtaca acctgcccaa ggacccgcag atcagcccgc tggtgagcct
      901 gtggaagggc gtgggcaagc cgatcgcgct gctggcgctg gcggcggcgg cgatcgggtc
      961 gttcttccac tacgtcaagg tgggaccgaa cgaggtcgag gaagaggaga ccagatgagc
     1021 ggaggcggcg tgaaaacatc gcgtaggccg gatgcagcgc agcggaatcg ggggaaaacg
     1081 aacggggcaa ttttatgaag agcaatctca tcgagcgcta cggcgcggag gatcgcatca
     1141 atcactgggt gatcgcgatc accttcgtcc tgctcgccct gtccgggctg gcgctgttcc
     1201 acccgtcctt gttctggctc accaacctgt tcggcggcgg cccgtggacg cgcatcctgc
     1261 atcccttcgt cggcgtggtc atgttcgtcg ctttcgcctg gctggcgctc ggcttctggc
     1321 gccacaacgt gctgggcgac aacgaccgcc gctggttgaa gcagtggcgc gacgtgatga
     1381 acaaccgcga agaccgcctg ccggaagtcg ggcgctacaa cgcggggcag aaggtgctgt
     1441 tctgggcgat ggtcgtgtgc atggcgatcc tgatcgtgag cggcatcgtc atctggcagc
     1501 cgtacttcgc gccggtcttc ccgatcgcac tgatccgcat cgcatcgctg ttgcacgcgc
     1561 tggccgcctt cgtgctgatc ctgggcatca tcgtgcacat ctacgcggcg ttgtgggtca
     1621 aaggcagcat cggcgcgatg gtccgcggca ccgtcacgcg tgcctgggcg cgcaagcatc
     1681 atccgggctg gtatcgcgag atcacggggg actgaatgcg cccttaaggc gcgtcttaag
     1741 aagaagagcg cccggccttt caccaagcgg agtggtgccg aagcccgggg cggtgtgggg
     1801 ttcatcgggt aaacctaatg tccgaaatca tccgcgacgg ctccctcgcc ggcggcatcc
     1861 ccactgtccc gcgcttccgt gccgtcggcg ccggcgtgtt cgcggagcgc gcggcgcgcc
     1921 tgcgcacgct cgcggccggc catgcgctgg agggctacct gcggttcgcg gccgcgctca
     1981 tcgacgcgca agacaaagcg ctgcgcgccg ccacgaattc ctcctcgtcg gggtggagtt
     2041 ccgcgcccga cgagcaaacg ctgcagcgtt gccgcgagca cggcctgccg ccgttgtcgg
     2101 tcgatggcca ccggcgcgac gcggtatggc gcgaagtcct cgccgggatt gccgggacct
     2161 tgaacccgga tgcgctgccg ccggaagcgc gcaacgtgct gtcggcgctc gccgccgcgc
     2221 cggtcgactc gctcgagtcg gcggcaagca gcctgctcgc cggcgcgtac gccgaggtcg
     2281 atgcgggatg ggctcctttc atcgccgccg cgctgcaggt gtactgggtg aggatggcgc
     2341 tgcggctcgg cgaggccggc ttcagccccg acgtgcgtta cggcctgtgt ccggtatgcg
     2401 gctcgcatcc ggtggcgagc gtcgtgcgcg tgggcggtgc ggcgcaggga ttgcgttacc
     2461 tggtgtgctc gctgtgcgcg tccgagtggc acgtggtgcg cgtgaagtgc gcggcctgcg
     2521 cctccaccaa gggtatcggc tacctcggca tcgagggcgc gagcccggcg atcaaggccg
     2581 agtgctgcga cgagtgccgc gcctacctca agatcttcta tctggagaag gacaccgcgc
     2641 tcgacccggt cgccgacgac ctggccacgc tcgccctgga catgctggtt gacgagcagg
     2701 gctacgaccg cgccgggccg aacctgctgt tcctgccggg cgccgtgtag cgcgtttcag
     2761 acgaagctcg cctccaccac gccgaacggc tcgaactccg agcgcacgtg cgcgggttgg
     2821 tcgagcgggt actgccgcgt gaaagatccc gagatcacgc gctgcccggc ctcgagggcg
     2881 cgcccgaact ccgccagctt gttggccagc cagacgatcg agttgatcgg gttgcccatg
     2941 acctgcgcgc cgtcggcgcg atcgatcagc gcgtggtcca cgtacacgcg caccgtggcg
     3001 ccggccagat cgtaatcccc gagcggcctc gccgccgcgg tgacgaaggc cttctgctgc
     3061 gcgttgtcgg ccatggtgag cggcagatcc gccgcgaaat cgccgcgctt ctccacgatc
     3121 tccagcgccg gcgccacctc ggcgatcgcg ctgcgtgcct ggtcgaaggt gacaccgggg
     3181 ccacgcagcg tgctgccgac ggtcaggcac agctcgttct cgaagcccgg tttgatcagc
     3241 tcggcgtgcc ggaacgcatg gccagaaggc cgattgccgc tcgccaggag gaagccgaag
     3301 cagggctcgt gcaccccgtg ctgcgcgcgc atggccgcag cggtgagccc caccttccag
     3361 cctgcgtgcc gctcgcccgc cacccgcagg cgctcgagga tcccgagctg gatccggtag
     3421 ccgtccacca tgctgagccg ccggctccat tccagcgggt agtgcacgcc ggcctgcagg
     3481 tggcgccaga tcgactcggt ggcggcgtcg atatccaggg tgggcatcgt gcgtggctcg
     3541 tgggcttgcg ctgcgagtat agcagcggcc gtccttcggc cccttcccct gaccctctcc
     3601 cgcaacggga gagggcactc ggggcgtccg aacgcgcgta gaattccttc gtcctcccgc
     3661 cgagcttcgt cgtgaacgag ccgctgccga ccctgctgtc tccccgtccc gggcccgccg
     3721 ccctgccttc cgtggatcga ctggtcgggc tcgcgcgcgt ggcgcagctg gtcgatgcct
     3781 acggccgcga cgcggtctat gcggccgtgc gcgcgttgct cgaagagaag cgccgcgcgg
     3841 tgctcggcgg tgcgctgccc gagtcggtat ggccacccgg agagatctgg gtggaggccg
     3901 acctgcgggc gcggctggag gccgatgcgg cgctgaagct gcgccccgtg ttcaatctca
     3961 ccggcacggt cttacatacc aacctgggcc gcgcgccgct gcccgaagag gcggtcgccg
     4021 ccgtcgcgaa agcgatgcgc ggcgccgtca acctggagta cgacctcgcc tcgggcgcgc
     4081 gcggggagcg cgacgagctg gtcgagggcc tgctcaccga gctgaccggc gcgcaggcgg
     4141 cgaccgtggt gaacaactgc gcggccgcgg tgctgctggt gctcaacacg ctcgccctgc
     4201 gcggcgacgt ggtcgtctcg cgcggcgagc tgatcgagat cggcggcgcg ttccgcatgc
     4261 cggacatcat ggcgcgcgcc ggcgcgaagc tggtcgaagt gggcaccacc aaccgcacgc
     4321 acgccgccga ttacgagcac gccatcgggt tgcgcaccgc gctggtgctg aaggtgcatc
     4381 cgagcaacta cagcgtcgag gggttcagca cgctggtcga cgaggaggag ctggcgcgca
     4441 tcgcgcaccg tcaccagctg ccgctgttcg tggatctggg cagcggcgcg ctggccgacc
     4501 tgcgggagtt cggattgccc ggagagccga cggccaaggc gttgctcgcg gccggcgccg
     4561 acctggtggc cttcagcggc gacaagctgc tgggcgggcc gcaggcgggg atggtggtcg
     4621 ggcgcaagga actgatcgcg aagctgcgca agaatccgct caagcgcgcg ctgcgcgtgg
     4681 acaagatgac gctggcggcg ctggaggcgg tgctgcgcct gtatcgcaat cccgaccggc
     4741 tcggccggcg cctcaccacg ctgcgcctgc tgacgcgcag ccaggcggag atcgtggcgc
     4801 tgggcgaacg gctgctggag ccggtgcgcg cagcgttcgc cgcgcaggcc gacgtctcgc
     4861 tgcaggagtg tcagagccag atcggcagcg gctcgctgcc ggtggaccgg ctgccgagcg
     4921 cggcgctggt gctgaccccg cgcgtgcagg gcaagggcga gggcacggcg ctgaaggcgc
     4981 tggagacgcg cctgcgcgat ctgccggtgc cggtgatcgg gcgcgtgcac gagggcgcgc
     5041 tgtggctcga cctgcgctgc ctggaagacg aggcccagtt cctggctcag ctcgcaacat
     5101 gatcgtcggc accgccggcc atatcgatca cggcaagacc gcgctgctca aggcgctgac
     5161 cggcgtcgac gccgaccgcc tgcccgagga gaaggcgcgc ggcatcacgc tggacctcgg
     5221 ctatgcctac acgccgctgc ccggcggcga ggtgctgggg ttcgtggacg tcccgggcca
     5281 cgagcggctg gtgcacaaca tgctggcggg cgccaccggc atcgacttcg tcctgctggt
     5341 ggtcgccgcg gacgacggcc cgatgccgca gacgcgagag catctgcaga tcgtcgacct
     5401 gctcggcctg tcgcacggcg ccgtggccgt caccaagatc gacgcggtga gcgcggaacg
     5461 tgcggcgcaa gcgcaagacg aggtgcgccg cctgctgcga ggcaccgcgc tcgaaggcgc
     5521 gccgttgttt cccgtgtcca gcgtgaccgg cggcggcgtc gatgcgttgc gcgcacacct
     5581 cgggcgagcc gccggcgagc ccggcgggcg cgacagccgc ggccacttcc gcctcgcgat
     5641 cgatcgctgt ttcaccctga aggggaccgg cacggtggtg accggggcgg tgcactccgg
     5701 caccgtgcgc gtgggcgacg agctgctgct ttcgccgccg gggattccgg tgcgcgtgcg
     5761 cggcatccat gcgcaggacc gtgcggcgga agccggcctc gccggacagc gctgcgcgct
     5821 caacctggca gggccggagt tcgaccggca gatcgtgcag cgcgggcact gggtgctgga
     5881 gccggcgctg cacgcgccca cgcagcgcat cgacgtgcag ttgcgcctgc tcgacagcga
     5941 gccgcgcgcg ctgaagcatt ggacgccggc acacgtgcac ctcggcgcgc gcgacgtgcc
     6001 ggggcgcgtc gcgctgctcg agggcgaaac cctcgagccg ggtgccaccg cgctcgcgca
     6061 gctcgtgctc gaccggccgg acgcggtcct ggcgggcgac cgcttcgtgg ttcgcgacca
     6121 gtccgcctcg cgcactatgg gcggcggcgt ggcactggat ccgttcccgc cgcagaaagg
     6181 acgccgcgcc tcgggacgtc tgtcgctgtt gcgtacgtgg cgatccgggg acgcgcgcgc
     6241 ggcgttcacc gctgcgctcg gcacggcggc gctgggtatc gatgtgcagc gcttcgcgct
     6301 cgcctggaac ctgcgcgaag aggagcggcc agccctgttc gacggcgtgg cgatgcgccg
     6361 ggtgcgcagc gccgccaccg acctggtttt ctcggcgagc gcctggcagg ggctgcgtca
     6421 agcgctgctc gcagcagtcg cggccgagca cgggcgcgcg cccgacatgg tgggtgtgag
     6481 ccgcgagcgg ctgcgccggc tgggggcgcc gtcgctggcg cccgcggcct acgaagcgtt
     6541 gctcgacgaa ctgctgggcg aaggcctgat tgtcgccagc ggcgcctggc tgcacgatcc
     6601 ggcgcatcgc gtgcgcttcg gcgcgcagga ggaacaccta tggtcgaagg tgcggccgat
     6661 gctggacgcg acgccattcc atccgccgcg cgtgcgcgac ctggcgcatg ccctccacgt
     6721 cgaagaggag gcgatgcgcc ggctgctgca gcgcgctgca cgccttggcg tggtgtatcc
     6781 ggtggcgcac gaccactact tcacgctgca cgccgtggac gccctggcgg agatcgtgcg
     6841 cgatctcaac cgcgcgaagg gtgcggcgct tgcggccgag ttccgcgacc gaatcggtac
     6901 cggtcgccgg ctcgcgatcc agatcctgga gttcttcgac cgcgtcggct acacccgccg
     6961 cgccggggac gcgcacctgc tgcggcagcc ggcgctgttc gctgcgcgag aggcccctct
     7021 ccccttgcgg gacaggggcg ggggtgaggg ctaaatcaag agggaagaga agcgtccccc
     7081 ggtggggcgt tcggacttca aatccgagaa gggccggcag cccggtcctt ggtgggttcg
     7141 actcccactc tcttccgcca cgctgcacgg cgcgtgctag catttaccga tgaacgacag
     7201 cacgcggccc atcgccgcct tcggcgagta cgcagtccag cgctgggacg gcgcgcgtgg
     7261 cgcgtccaac gtcgatcgcg tcgcggaaga agttcccgtc tcactggtgt acaacggcct
     7321 ggcgcatgcc gtgatgatgg cctcgccggc ggacctggag gacttcgcgc tcggcttcag
     7381 cctgagcgaa ggcatcgtgg cgtcgcccgc ggagctgttc cgcgtggacg tggtgccgct
     7441 cgacgaaggc atcctgttgc gcatggagat cgagccggcg cgctgcgtac cgttggagga
     7501 gcagcgccgt cacctcgccg ggcgcacggg atgcgggctg tgcggcgccg agagcatcga
     7561 gcaggcggtg cgccaccccg cgcgggtcag tggcggaccg cgcattgcgc ccgccgcgct
     7621 gcacgcggcg ttcgccaggc tggcgcagag tcaaccgctg agcgccgtca ccgggggcgt
     7681 gcatgcggcg gcatgggccg gcgtcgacgg tgagctgctg gccgtgcgcg aagatgtcgg
     7741 ccgccacaac gcgctcgaca agctgatcgg cgcgctggtg cgcggcgggc ggcgcatgga
     7801 cgaaggcttc gccctgatca ccagccgcgc gagcttcgag atggcgcaga aggcggccac
     7861 ggtggggatc accctgctcg cggcaatctc cgctcctacc ggcctcgcca tccgcctggc
     7921 cgaggaaacc ggtctcaccc tggtcgcctt cgcgcgcgac ggcaagcaca acgtgtacgc
     7981 gaatccggcg cggatcctag tcgcctagca cccgccgcac gaacgcttcc atcgccgcca
     8041 gcaccgcgtc cggctgatcc ctgtgcggcg agtggccgca gtcggccagt ttgaccacgt
     8101 ccacgtcgcg cgcctggcgc gcgatgcgct cgatctgctc catggtgccg tactcgtcgt
     8161 cctcgccctg gatggcgaga atcgggcacg cgatgctggg caggcagctc tcgatgttcc
     8221 attctcggaa cgcggggtcg agccagatgc ggttccagct ccagaacgcc gcgtccgggt
     8281 cggcgtgatg gcgcgacagg cgctggcgca gatcggtggt ctcgtaggca acccgcgcct
     8341 gggcgatgct cgccaccgag agctcctcga ccatcacgtg cggcgccatc gcgatcacgc
     8401 cgctcggagc cggggacgcg ctgccggcat ggatcaacgc gatcgaggcg ccgtcgctgt
     8461 ggccgagcag gatcggccgc tcgatgcgca gccgttccag cagcgcgggc agcacgatca
     8521 gcgcctcgtc gtgcatgtag cgcaccggca gcggcgcggt cggtggatcg gagcggccgt
     8581 agccttggcg ggagtacacc acggcctcgc agccggtggc atcggcaacg cgctgcggga
     8641 agtcgcgcca catcgacacc gagcccaggc cttcgtgcag gaacacgatg ctcggcgcgg
     8701 cggcccgggc gtgcgaagcg gccaggcgca cgcactcgag ccgttgcccg gcggcttcga
     8761 cccactgcat gctagggccg cgccagccgc gcgcgcagct tgaagcgctg gatctttccg
     8821 gtcgcggtct tgggcagctc gtcgatgaac tcgacccagc gcggatactt gaacggcgcc
     8881 agccgcgtct tgacgtgctg ctgcagctcg gcggccaacg ccggcgtggc gccgcgcccg
     8941 ggcttcagca ccacgtaggc catcggcttg gtcagcttgt cgtggtcctc gcgcccgacg
     9001 accgcggttt ccagcaccgc ttcgtgggtg atcagggccg attccacctc cacgggagaa
     9061 acgtacatcc cgctcacctt gagcatgtcg tcgctgcgcc ccgcgtacac gtagtaaccg
     9121 tcggcgtcct gcaggtactt gtcgccgctg cgggtccacg ggccctggaa ggtggcgcgc
     9181 gtcttgtcgc ggttgttcca gtagaacgcc gcgctggtcg gcccgctgat ctgcagctcg
     9241 ccctgctcac cgggcgcgac cggcatgccg tactcgtcgg tgagccgcac ctcgtaaccc
     9301 gggaccggct tgccggtggt gccgtagcgc acatcgcccg ggcggttgga caggaagatg
     9361 tgcagcatct cggtgctgcc gatgccgtcc aggatgtcca cgccgaagtg cgccgaccag
     9421 cgcttgccga tctcctcggg cagcgcctcg ccggccgagg cgcagcggcg caggcgcagc
     9481 gcgccgcgct cgggcagctc cgggctcgcg agcagcgccg cgtagagcgt gggcacgccg
     9541 tagaagatgg tgggctggtg cttgcgcaac cgcgcgaaca ccgatgcggg cgtgggccgt
     9601 tcggccatca gcacggcggt ggcgcccacc gccatcggga aagtgagcgc gttgcccagc
     9661 ccgtaggcga agaacagctt ggcggccgag aacaccaggt cgccttcctc gatgcccagg
     9721 atcgggcgcg cgtaaagctc ggccgtttga atcaggctgc cgtgcacgtg caccgttccc
     9781 ttgggcgatc cggtcgagcc cgaggagtac agccagaagc aggcgtcgtc cgcggtggtg
     9841 ggcgcggggg cgaaggcgcc gcgcgccgaa tccagcagcg atacaaatgg gcgatgcccg
     9901 gcggcgtcgc cgcccgacac gatcacgtgc tcgaggaacg gcaggcgcgg gagcaggggc
     9961 gcgaaggtcg cgagcagcgg cgccgacacg atcagggcgc gcgcacggct gtcgcgcaac
    10021 atgtattcgt agtccgcggc ggtgagcagc gtgttg
//
LOCUS       DASPGE010000066         4554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1543395, whole genome shotgun sequence.
ACCESSION   DASPGE010000066 DASPGE010000000
VERSION     DASPGE010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543395"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..788)
                     /gene="pgsA"
                     /locus_tag="VD791_02080"
     CDS             complement(216..788)
                     /gene="pgsA"
                     /locus_tag="VD791_02080"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HXV06788.1"
                     /translation="MPANIPNLLTWLRIILIPLFLAIYYTPDTWLSGPAKNMTATAMF
                     AFAAITDWLDGYLARSLNQTSAFGAFLDPVADKLMVAAALILLVSLQRVDAIIAVVII
                     GREIAISALREWMAKLGQSRSVAVHFLGKVKTGCQMAAILLLLFNGILLGVDTQQVGT
                     VLIYIAALLTLWSMGYYLRRAMPWITGKET"
     gene            complement(788..2677)
                     /gene="uvrC"
                     /locus_tag="VD791_02085"
     CDS             complement(788..2677)
                     /gene="uvrC"
                     /locus_tag="VD791_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014722731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HXV06789.1"
                     /translation="MSKVPAHARAYTPAPSSGATTLLSAPAAAGTDLAEVVAGLPHLP
                     GVYRMLNAAAEVLYVGKARDLQKRVASYFQKTASLEPRIQLMVAQVAAIETTVTRSES
                     EALLLENNLIKTFAPRYNILYRDDKSYPYLQVSGHRFPKLGFYRGPLDRVNRYFGPFS
                     SAHAVRESIQLLQKVFRLRTCEDTVFSNRSRPCLLHQIRRCTAPCVGLIDAASYAEDV
                     RNAELFLLGRDDEVLAKLEERMQAAAAGLDYEAAAMYRDQVRALRGVRQTQFVSSERA
                     RDVDIVALAAASGVLCVNLVTIRGGMHRGDKSFFPEHGEGYEAGQALQAFLSQHYLQR
                     EVPPLIVLNHEVDGEALARLLSEQSGHEVQISASATGERRAWLAMAETNARVAIGQRL
                     QLHATQEARLAALLEALGLPATTQRIECFDISHTGGEATVASCVVWDEGAMRKDQYRH
                     YNIQGVVPGDDYGAMREVLDRRYRKVVAGEGRMPDLILIDGGKGQVSSAMAVLEELGL
                     AQVALIGVAKGEERKPGQEQLIVPGRHEPLRLPKDHPGLHLIQQIRDEAHRFAITGHR
                     GRRGKTRRTSTLEAIAGVGAKRRQRLLARFGGLRGVLTASIDEIAQVDGIGRDLAERI
                     YQHLH"
     gene            2851..3417
                     /gene="efp"
                     /locus_tag="VD791_02090"
     CDS             2851..3417
                     /gene="efp"
                     /locus_tag="VD791_02090"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXV06790.1"
                     /translation="MAKVLATEIRAGNLLEWDKRIWRVLKSYHVHVGGRGGAFMQVEM
                     KDIEAGTKANQRFRTDEKVERAFVESREMEYLYNDGTGYVFMDKENFEQLNLSAELLE
                     GQVEYLLPNTVVQVNFHNERPIGVQLPPSVVLTITETEPTLKGSTATGSFKPAKTETG
                     LTVMVPPFVGEGEKIKVNTDSGEYVERM"
     gene            complement(3544..4455)
                     /locus_tag="VD791_02095"
     CDS             complement(3544..4455)
                     /locus_tag="VD791_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXV06791.1"
                     /translation="MHDQPHEHRHGAAMGSAALMWAMALTVGYAAVEFAAGWWFGSLA
                     LMSDAGHMVSDAAALGLAWFAAWLSQRPPGSRHTFGLARAEVIAAFVNGLGLLLVVLL
                     IAVEAVKRLLAPPAVQGLGVMAVALVGLLLNLGVAFLLSRSERTLNRRAALLHVLADL
                     VGSFAALLAGAVIYFTGWRPIDPILSLAIALLILGSTIGLLREALHVLMEGVPRSIRL
                     DEVGKALARIADVHDVHDLHIWHISSGQVALSAHVRLASLERWPEVLEEARAILERRF
                     GIAHVTLQPELSARASEESVIRLFTRR"
BASE COUNT          747 a         1588 c         1547 g          672 t
ORIGIN      
        1 agttacacgc atgggtcgaa gtctcgaggg attgcgttgg cagccagtat gcagaacccg
       61 accttaaagc aagctgaatc gctcgaggac tgccgatccg aggtcgctct atgactccaa
      121 ctacttgaaa gtttggaggc gtcggggcga gcgcagcgct gctctcaccc tccaacgcca
      181 gcccctgtcc gcagggagac aggcggcgtg tgcaatcagg tttccttgcc cgtgatccac
      241 ggcatcgcgc ggcgcaggta gtagcccatc gaccacagcg tgagcagcgc ggcgatgtag
      301 atcagcacgg tgcccacctg ctgcgtgtcc accccgagca ggatgccgtt gaacagcagc
      361 agcaggatcg ccgccatctg gcagccggtc ttgaccttgc ccaggaagtg caccgccacg
      421 ctgcggctct gcccgagctt ggccatccat tcgcgcagcg ccgagatggc gatctcgcgg
      481 ccgatgatga ccacggcgat gatcgcatcc acgcgctgca ggctcaccag caggatcagc
      541 gcggcggcca ccatcagctt gtccgccacc gggtcgagga aggcgccgaa ggccgaggtc
      601 tggttgagcg agcgcgccag gtagccgtcc agccaatcgg tgatggcggc gaaggcgaac
      661 atcgcggtcg ccgtcatgtt cttcgccggg ccgctgagcc aggtgtccgg cgtgtagtag
      721 atggccagaa acagcgggat caggatgatc cgcagccagg tgagcaggtt ggggatgttc
      781 gcgggcatca gtgcagatgc tggtagatgc gctcggccag atcgcggccg atgccgtcaa
      841 cctgcgcgat ctcgtcgatg ctcgccgtga gcaccccgcg cagcccgccg aagcgcgcca
      901 gcagcctctg gcggcgcttc gcgcccacgc cggcgatcgc ctccagggtg gaggtgcgcc
      961 gcgtcttgcc gcgccggccc cgatggccgg tgatggcgaa gcggtgcgcc tcgtcgcgga
     1021 tctgctggat caggtgcagg cccgggtgat ccttgggcag gcgcagcggc tcgtgccggc
     1081 cgggcacgat gagctgctcc tggccgggct tgcgctcctc gcccttggcc acgccaatca
     1141 gcgccacctg cgccaatccc agctcctcca gcaccgccat cgcgctcgac acctggccct
     1201 tgccgccgtc gatcaggatc aaatcgggca tcctgccctc gcccgcgacc accttgcgat
     1261 agcgccggtc caggacctcg cgcatggcgc cgtagtcgtc gccgggcacg acgccctgga
     1321 tgttgtagtg gcgatactgg tccttgcgca tggcgccctc gtcccacacc acgcaggagg
     1381 ccacggtcgc ctcgccgccg gtgtggctga tgtcgaagca ttcgatgcgc tgcgtcgtgg
     1441 ccggcaggcc cagggcctcc agcagggcgg ccaggcgcgc ctcctgcgtg gcgtgcagtt
     1501 gcagccgctg gccgatggcg acccgggcgt tggtctccgc catggcgagc caggcgcgcc
     1561 gctcgccggt ggcgctggcg ctgatctgca cctcgtgccc ggattgctcg gacagcaacc
     1621 gggccagggc ctcgccgtcc acttcgtggt tcaggacgat gagcggcggc acctcgcgct
     1681 gcaggtaatg ctggctgagg aaggcctgca gcgcctgccc ggcctcgtag ccctcgccgt
     1741 gctccgggaa gaaggacttg tcgccgcggt gcatgccgcc gcggatcgtc accaggttca
     1801 cgcacagcac gccggatgcc gccgccaggg ccacgatatc cacgtctcgc gcgcgctcgc
     1861 tgctcacgaa ctgggtctgg cgcacgccgc gcagcgcgcg cacctgatcg cggtacatcg
     1921 ccgcggcctc gtaatccagc ccagcggccg cggcctgcat gcgctcctcg agcttggcca
     1981 gcacctcgtc gtcgcggccg agcaggaaga gctcggcgtt gcgcacgtcc tcggcgtagc
     2041 tcgccgcgtc gatcaacccg acgcaaggcg cggtgcaacg ccggatctgg tgcagcaggc
     2101 aggggcgcga gcggttgctg aacaccgtgt cctcgcaggt gcgcagccgg aataccttct
     2161 gcagcagctg gatgctctcg cgcacggcat gcgcgctgga gaacggtccg aagtagcggt
     2221 tcacgcgatc cagcgggccg cggtagaagc ccaacttggg gaatcggtgt ccgctgacct
     2281 gcagataggg ataggacttg tcgtcccggt agaggatgtt gtagcgcggc gcgaaggtct
     2341 tgatcaggtt gttctcgagc agcagcgcct cggactccga gcgcgtcacc gtggtctcga
     2401 tcgcggccac ctgcgccacc atgagctgga tgcgcggctc caggctggcg gtcttctgga
     2461 agtaggaggc cacgcgcttc tgcagatcgc gcgccttgcc cacgtaaagc acctccgccg
     2521 ccgcattgag catgcggtag acgccgggca gatgcggcag gccggcgacg acctcggcga
     2581 ggtcggtgcc cgcggccgcc ggggccgaca atagcgtcgt cgctccggaa gagggcgccg
     2641 gcgtgtaggc gcgggcgtgg gcggggacct tgctcatgcg ccggggagga agatcggcgt
     2701 gcctcggaat gggccggggg tgcaggctgt ggcgggtggt gcgtggtgcg tattatagcg
     2761 gcggccgcgc gcgcagcctt aggcacggcc ggcgtcttcg ggtagaatcc gcacctttcc
     2821 gcggaactcc gcagccgcat atccccaaac atggccaagg tcctcgccac cgaaatccgc
     2881 gccggcaacc tgctcgaatg ggacaagcgc atctggcgcg tgctcaagtc ctatcacgtg
     2941 cacgtcggcg ggcgcggcgg cgcgttcatg caggtggaga tgaaggacat cgaggccggc
     3001 accaaggcca atcagcgctt ccgcaccgac gagaaggtcg agcgcgcctt cgtcgagagc
     3061 cgcgagatgg agtacctgta caacgacggc accggctacg tgttcatgga caaggagaac
     3121 ttcgagcagc tcaacctgtc cgccgagctg ttggagggcc aggtcgagta cctgctgccc
     3181 aacaccgtgg tgcaggtgaa cttccacaac gagcgtccga tcggcgtgca gctgccgccc
     3241 agcgtggtgc tcaccatcac cgagaccgag cctaccctca agggctccac cgccaccggc
     3301 tcgttcaagc ccgccaagac cgagaccggt ctcaccgtca tggtgccgcc gttcgtcggc
     3361 gaaggcgaaa agatcaaggt caacaccgac tcgggcgagt acgtcgagcg catgtgacgc
     3421 ccggccagcg cgcaggaggg gaagttactc ccctcctctc atgaggaggg gtcggccgaa
     3481 ggccgggggt ggtgtggctt tcgtcccaca cttacgaaaa cctcaatagt tcgccacgcg
     3541 ccgtcagcga cgggtgaaca accggatcac cgattcctcg ctggcgcgcg cggacagctc
     3601 cggttgcagc gtgacgtgcg cgatgccgaa gcgccgctcc aggatcgccc gcgcctcctc
     3661 gaggacctcc ggccagcgct ccaggctcgc cagccgcacg tgcgcggaca acgcgacctg
     3721 ccccgacgag atgtgccaga tgtgcagatc gtgcacgtcg tgcacgtcgg cgatgcgcgc
     3781 cagcgccttg cccacttcgt ccagccgaat gctgcgcggc acgccctcca tcagcacgtg
     3841 cagcgcctcg cgcagcaggc cgatggtgga gcccaggatc agcagggcga tcgccagcga
     3901 caggatcggg tcgatcgggc gccagccggt gaagtagatc accgcaccgg ccagcagcgc
     3961 ggcgaaggag ccgaccaggt cggccagcac gtgcagcagc gctgcgcgcc ggttgagggt
     4021 gcgttcgctg cggctcagca ggaacgccac ccccaggttg agcaggaggc cgaccaaggc
     4081 gaccgccatc acgcccagcc cctgcacggc gggcggcgcc agcagccgct tgaccgcctc
     4141 caccgcgatc agcagcacca ccagcagcag cccgaggccg ttgacgaacg ccgcgatcac
     4201 ctccgcccgc gccagcccga aggtgtggcg cgatccgggc gggcgctgcg acagccaggc
     4261 ggcgaaccag gcgagcccca gggccgcggc gtccgacacc atgtgcccgg catcgctcat
     4321 caacgccagg gagccgaacc accagcccgc ggcgaactcc accgccgcgt atcccaccgt
     4381 gagcgccatc gcccacatca gggccgcgct gcccatcgcg gcgccgtgcc ggtgctcgtg
     4441 gggttggtcg tgcacgtggg ccactagcca gtctctcccc gctgcacaac ctccagcgcg
     4501 gcgcgcgcgg cacaccagtc cgcatcttcg gccagcgcgc cgaggccgca ggcc
//
LOCUS       DASPGE010000067        11455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_841225, whole genome shotgun sequence.
ACCESSION   DASPGE010000067 DASPGE010000000
VERSION     DASPGE010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11455
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841225"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VD791_02100"
     CDS             <1..197
                     /locus_tag="VD791_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06792.1"
                     /translation="AYDKTKEMAVKGYESTKKVLTAPPTATGPEHYGRAGGYVGMEKF
                     QTQPESQTFAHESRGIPPRE"
     gene            complement(253..1044)
                     /locus_tag="VD791_02105"
     CDS             complement(253..1044)
                     /locus_tag="VD791_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXV06793.1"
                     /translation="MSDQPQIIVRREGTVGRVLFSNPARRNAMTVDMWRAVPGALQEL
                     DADAVIRVIVLEGAGDVAFVSGADISQFESVRFDTDARRRYDDTVEAAYAALLVCSKP
                     VLAKIRGICMGGGLGLAAACDVRICADDARFRMPAGRLGLGYKLDGVRRFVALIGAQN
                     TLDIFFTARVFDAQEALRMGFVGKVAPVERLEDEVAQWCVQVADNAPLTLRAAKLAVN
                     ALLAPDAERDLQAVETALEACFRSEDYREGVRAFLEKRNPRFKGR"
     gene            complement(1045..2238)
                     /locus_tag="VD791_02110"
     CDS             complement(1045..2238)
                     /locus_tag="VD791_02110"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXV06794.1"
                     /translation="MPRPVTTGALAHLRIVDLSQVRAGPTCVRQFADFGADVIKVESR
                     AGSGRSDLMIGSRTGADMLNLHRNKRCITLDLKSEGGKGVLRKLVERADVVVENFRPD
                     VKHRLGIDYDSLAAINPRVILASISGFGQDGPYRDRPGFDQIAQGMSGLMSVTGYPDG
                     APTRVGAAVADVAAGLFAALGIMTALLERERSGKGQWVQSSLLHAGIALMDFQAARYL
                     IGGEVPKPVGNDHPTSMPTSTYPTRDGYLNVAASGQAMWKTLCTTLGREDLLQRQEFA
                     DEAGRSRNRAALNAELATALRTRTNGEWIERLNAAGIPCGPIYRVDEVFADPQVQHLG
                     IATPVPHPQLGEIRVVGNAVRLSRTPANLVTALPDAGAHTDEVLEELGYTRAQIEALR
                     RDGAI"
     gene            2434..3069
                     /locus_tag="VD791_02115"
     CDS             2434..3069
                     /locus_tag="VD791_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HXV06795.1"
                     /translation="MNLLHFGLTAFVTLLVVVDPVGVAPIYVACTRELEPGARRDTLL
                     RAIVVALAVTLFFLFGGRLLLIYLGVSVHAFAISGGILLFATAVPMLFGQRPMLQGPE
                     RGDYGKVGEDVAVFPLAIPLLSGPGAIATTLLLTSQAAGDYARLSLLASVIGLVYLIA
                     WLILRTADQLMARLGEGRIHILTRVLGIVLAALAVQYVLDGAAGYYRALTG"
     gene            3074..3661
                     /locus_tag="VD791_02120"
     CDS             3074..3661
                     /locus_tag="VD791_02120"
                     /inference="COORDINATES: protein motif:HMM:NF016239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HXV06796.1"
                     /translation="MNFLAHLYLADRRPDALLGSLMGDFVKGPLQGRFSPELTQALAQ
                     HRKVDVFTDAHPVVRASRARMAPARRRFAGIMVDVFYDHFLALHWEDYADQPLEAFAR
                     EVYALLRTREAILPERLRRIAPLMAQFDWLGSYRRVEAVHVALDRMGERLKRGNALLG
                     AGAELNANYAGLEADFRAFFPEVVRFAREDLGTRI"
     gene            complement(3731..5716)
                     /locus_tag="VD791_02125"
     CDS             complement(3731..5716)
                     /locus_tag="VD791_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06797.1"
                     /translation="MRRFALLLLLLLAACAGKREARDRMDWGELRELFPPTLSQPSAA
                     SPSTAEATADAEATAAAAAADATAAAASAQAAAAEAPPQSVDNEPAQDATAPRESAAA
                     SSAPSAGADVEPDPHAPDTSAAASIGASAAGAEPMSASNVAAELAAAEPTVMPDVAAA
                     PEPAAAAAAAAKAPQWPHTVAANGVSFEIHAPQVDAWDGEALLAHALVKAQPGGRKQP
                     AIGAVRMTARTEVDDGAGTVALQDLEVTAARFPGAPDKTQAAWLETLRAGLPQQVNGV
                     ALERVESGIPAVRARERGAAVLSVVAPRVVIAKKPTVLVYIDGEPRYVPVKDTQLSGV
                     LNTRTLLLRDAKGRHYLRLYDGWARAESLAGPWSVSKPPPASTQVAQVARASGRVNLL
                     QGKPDPKTRRRPSLKSGSLPQILVATKPTALLVLDGEPRFEAVAGTSLQYATNTTAHL
                     FRDVETKQLYVRIAGYWFRSGSTAGPWEFVSAHRLPESFAAIPDDSPKRGVKAGIAAA
                     KQEHAAPARGIAPVMVAASPTRTSLYLTLDGDPRLEPVEGTQLGYVANASVPVIRLDD
                     RRWFAIQNGAWFSAETVAGPWRLADTIPPDIYAIPPSAAIYPAIHSRVYSSSSDVVYF
                     AYEPSLLGISGGAAGVEDQGEDYQYTPPAGMHWGWFY"
     gene            complement(5853..6923)
                     /locus_tag="VD791_02130"
     CDS             complement(5853..6923)
                     /locus_tag="VD791_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXV06798.1"
                     /translation="MTRVIAPCACLALIAFAGGAQAKSVCETAGISTRELATESYRIA
                     SDTPGIELYLRNKRPVQVKSFAAERVLLYVHGATYPAETAFDLPLAGMSWMDYIACHG
                     WDVYLVDLRGYGQSTRPAEMARPAEEGEPIVTTDVAVRDVGTAVEHVLRRRGVTRLNL
                     LGWSWGTAIMGTYAAGNPDKVARLVLYAPLWLRQTPSLVAGDGPLGTYRTVTQEQARK
                     RWLTGVPDTARHDLIPEGWFEAWAQATWATDPDSGKWNPPLLRAPNGVVRDGREYWSA
                     GRPYYDPRAITAPTLLIHGEWDQDTPGYMAQSVFALLEKAAWKRLVVIGAGTHTVIME
                     ANRLQLFREVQLFLDEPAPRAR"
     gene            6989..8014
                     /locus_tag="VD791_02135"
     CDS             6989..8014
                     /locus_tag="VD791_02135"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HXV06799.1"
                     /translation="MSSHPYDVLTPDLILDAVESAGLRCDGRLLALNSFENRVYQVGI
                     EEAQPVIVKFYRPGRWSDAQIAEEHDFARELAQAELPVVAPLAVDEGGGEGGGEAGGE
                     SGALRTFGGFRFALYPRQRGRAPELESQDTLTWMGRFIGRIHAVGALRPFAQRPALTI
                     ESFGEEPARFLLDSGFVPADLALAYRTLAEQLLATIRACFTRAGQSAAVRLHGDCHPG
                     NVLWADDGPHFVDLDDARMGPAVQDLWMLLSGDRDSMARQLRWLLDGYWEFHEFELRE
                     LQLIEPLRTLRMMHYAGWLARRWDDPAFPAAFPWFNTQRYWQDHILALREQAAAMDEA
                     PPAPAPG"
     gene            8082..8825
                     /locus_tag="VD791_02140"
     CDS             8082..8825
                     /locus_tag="VD791_02140"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HXV06800.1"
                     /translation="MNAPHRIVAPWGLKQVVPLLKTHRVQLTEPGMAPAFSLNLHAVP
                     LGASEFGVAMRDYPIVFLRRARDGVHQAVAILGMKEGENLYVMPDGRWDRRTYIPAYI
                     RRYPFHVEVTGAERTICVDPDAVADDGERLFDEVGAPLQHWIVFERLLNEYEDELARA
                     HRLGDKLHALGVLAPFSLHADLSSGFSLSLDGMFRVDRERLGALKAAALRELIGSGAM
                     ESVYAHLLSQENFRRLLSRRGFFAKRPAS"
     gene            9013..10290
                     /gene="phaZ"
                     /locus_tag="VD791_02145"
     CDS             9013..10290
                     /gene="phaZ"
                     /locus_tag="VD791_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HXV06801.1"
                     /translation="MLYELHELQKTVSAPLLALVHASHRLFSNPYSPLAYTPVSRHIA
                     ASSELITRLMRSYHKPAWGIDHALVAAEPPAEKRPGETPALPGQTLGVPVLIEPVLTR
                     PFCHLLHFRKQTDRPGPKVLIFAPLSGHHATLLRDTVRTMLRDFDVYLTDWVDARMVP
                     LARGPFHLEDYVDYCQEFIRFLGPGIATVSVCQPTVPVLAAIALLAQEQDACVPRAMV
                     MMGGPIDTRRSPTVVNELAMGRPHSWFEDQVIHRVPFNYPGFMRRVYPGFLQHLSFIA
                     MNADRHIEAHRRFFNHLIVGDGDSAESHRRFYDEYNAVLDMPAEYYLDTIKVVFQDFL
                     LAKGRWEVRGALVRPEAIRNAALFTVEGELDDISGNGQTEAAHGLCRNIAAEKRRHLL
                     AQGVGHYGIFSGRKFRDTIYPQIRDFIRRWTAV"
     gene            10348..10509
                     /locus_tag="VD791_02150"
     CDS             10348..10509
                     /locus_tag="VD791_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06802.1"
                     /translation="MFEENFANSLIKAEGNWLAFFERPIAGTLGVVTLLVWLSPLALG
                     GWRRRRAPA"
     gene            10677..11126
                     /locus_tag="VD791_02155"
     CDS             10677..11126
                     /locus_tag="VD791_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXV06803.1"
                     /translation="MATLKVNGKQVRKDVEPDMPLLWLLRDELGLTGTKYACGIAACG
                     ACTVHVDGQPVRACVMPVSAVEGKEITTIEGLSKDGSHPVQKAWVQLQVPQCGYCQSG
                     MIMAAAALLKANRKPSDADIDAAMTNLCRCGTYHRVRQAVHLAAKSA"
     gene            11139..>11455
                     /locus_tag="VD791_02160"
     CDS             11139..>11455
                     /locus_tag="VD791_02160"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXV06804.1"
                     /translation="MTYLIDSNRREFLVKSAAVAGGMMLGVRLPGAAQAAEGAAGPEV
                     THWIVIHPDDTVVVRIARSELGQGTFTGLAMLVAEELEADWSKVRAEYADVNEHVRRN
                     RIWG"
BASE COUNT         1662 a         3957 c         4047 g         1789 t
ORIGIN      
        1 aagcctacga caagaccaag gagatggcgg tcaagggtta cgagtcaacc aagaaagtgt
       61 tgaccgctcc gccaacggca acggggcctg agcactatgg gcgcgccggc ggttacgttg
      121 gaatggagaa attccagacg cagccggagt cgcagacttt cgcgcacgaa tcgcgcggca
      181 taccgccccg cgaatgacag tgggtgcagg caacgggggc cgcacggccc ccgggccctt
      241 gaatagtcca ggctaacgcc ccttgaaccg cgggttgcgc ttctcgagga aggcgcgcac
      301 gccctcgcga taatcctcgc tgcggaagca ggcttcgagc gcggtttcga ccgcctgcag
      361 gtcgcgctcc gcgtccggtg cgagcagcgc gttgacggcg agtttcgccg cgcgcagcgt
      421 gagcggagca ttgtcggcca cctgcacgca ccactgcgcc acctcgtctt ccagccgttc
      481 gaccggcgcc accttgccga cgaagcccat gcgcagcgcc tcttgcgcgt cgaacacgcg
      541 ggccgtgaag aagatgtcga gcgtgttctg cgcgccgatc agcgccacga agcggcgcac
      601 gccgtcgagc ttgtacccca gcccgaggcg accggccggc atgcggaagc gggcgtcatc
      661 ggcgcagatg cgcacgtcgc aggcggcggc caggcccagg cccccgccca tgcagatgcc
      721 gcggatcttg gccagcaccg gcttgctgca caccagcaga gcggcgtaag ccgcctcgac
      781 ggtgtcgtcg tatcggcggc gggcgtcggt atcgaagcgc accgattcga actgcgagat
      841 gtcggcgccg gagacgaagg cgacatcgcc cgcaccctcg agcacgatga cgcggatcac
      901 tgcatccgcg tccaactcct gcagcgcgcc cggcacggcg cgccacatgt ccacggtcat
      961 cgcattgcgc cgggccggat tggagaacag cacgcgcccc acagtgccct cgcggcggac
     1021 gatgatctgc ggctggtcgc tcattcagat ggctccatcg cggcgcagcg cctcgatctg
     1081 ggcgcgggtg tagcccagct cctcgagcac ctcgtcggtg tgcgcgccgg catccggcag
     1141 cgcggtgacc aggtttgcgg gcgtgcgcga caatctcact gcattgccga cgacgcggat
     1201 ctccccgagc tggggatgcg gcaccggcgt ggcgatcccg aggtgttgca cctgcgggtc
     1261 ggcgaacacc tcgtccacgc ggtagatcgg gccgcagggg atgccggcgg cgttgaggcg
     1321 ttcgatccac tcgccgttgg tgcgcgtgcg cagcgcggtg gcgagctcgg cgttgagcgc
     1381 ggcgcgattg cgcgagcgtc cggcctcgtc ggcgaactcc tggcgttgca gcaggtcctc
     1441 gcgtccgagc gtggtgcaca gcgttttcca catcgcctgg ccggacgcgg ccacgttgag
     1501 gtagccgtcg cgcgtcgggt aggtcgaggt cggcatgctg gtcgggtgat cgttgcccac
     1561 gggtttgggc acttcgccgc cgatcagata gcgcgccgcc tggaagtcca tcagcgcgat
     1621 gcccgcgtgc agcagcgaag actgcaccca ttggcccttg ccgctgcgct cgcgctcgag
     1681 cagggcggtc atgatgccca gcgccgcgaa cagaccggcg gcgacgtcgg ccacggcggc
     1741 acccacgcgg gtgggcgcgc catccgggta tccggtcacc gacatcaacc cggacatgcc
     1801 ttgcgcgatc tgatcgaaac cgggacgatc gcggtagggg ccgtcctggc cgaagccgga
     1861 gatgctggcg aggatgacgc gcggattgat cgccgccagg ctgtcgtagt cgatccccag
     1921 ccggtgcttc acgtccggac ggaagttctc gaccaccacg tcggcgcgct ccaccagctt
     1981 gcgcagcacg cccttgccgc cctcggactt gagatcgagc gtgatgcagc gcttgttccg
     2041 gtgcaggttc agcatgtcgg caccggtgcg cgaaccgatc atgagatcgg aacgcccgct
     2101 accggcccgc gactcgactt tgatcacgtc ggcgccgaaa tcggcgaact ggcgcacgca
     2161 ggtcggaccg gcgcgcacct ggctgaggtc gacgatgcgc aggtgcgcga gcgcgccggt
     2221 ggtcacgggg cggggcatgg tcggtgcggc agggcgctgg cgaagggctg gcgcaggaag
     2281 catatcccat ttccgcgccg cgccccatgc cggtaccatg caatgatccc tcacccccgc
     2341 cctctcccgt aaggggagag ggaatggcct tcgccgtcca caccggactg aggctcgttg
     2401 gcagtcgatt agagcacagg cctgacccaa cccatgaacc tcctgcactt cggtctgacc
     2461 gcattcgtga cgttgctcgt ggtggtggac ccggtcgggg tggcgccgat ctacgtcgcc
     2521 tgcacgcgcg agctggagcc cggcgcgcgg cgcgacacgc tgctgcgcgc catcgtcgtc
     2581 gcgctggcgg tgacgctgtt cttcctgttc ggcgggcggc tgctgctgat ctacctcggg
     2641 gtgtcggtgc acgccttcgc catcagcggc ggcattctgc tgttcgccac cgcggtgccg
     2701 atgctgttcg gccagcgccc gatgctgcaa ggccccgagc gcggagacta cggcaaggtg
     2761 ggcgaggacg tcgcggtgtt cccgctggcg atcccgctgt tgtccggccc tggcgcgatc
     2821 gccaccacgc tgctgctcac cagccaggcc gccggcgact atgcccggct gagcctgctc
     2881 gcatcggtaa tcggcttggt gtacctgatc gcctggttga tcctgcgcac cgccgaccag
     2941 ctcatggcgc ggctgggcga gggcaggatc catatcctga cgcgcgtgct cggcatcgtg
     3001 ctcgcggcct tggcggtgca gtacgtcctg gacggggccg ccggctacta ccgggcgctc
     3061 accggctgag gcgatgaact tcctcgcgca cctgtatctc gcggatcggc ggcccgacgc
     3121 gctgctcggc agcctgatgg gcgacttcgt caaggggccg ctgcaggggc gcttctcccc
     3181 cgagctcacg caagcgttgg cgcagcaccg caaggtggat gtgttcaccg acgcgcatcc
     3241 ggtcgtgcgc gccagccgcg ctcgcatggc gccggcgcgg cggcgcttcg ccggcatcat
     3301 ggtggacgtg ttctacgacc acttcctcgc cctgcactgg gaggactacg ccgaccagcc
     3361 gctggaggcg ttcgcccgtg aggtttatgc gctgctgcgc acgcgcgaag cgatcctgcc
     3421 cgagcgcttg cggcggatcg cgccgctcat ggcgcagttc gactggctcg gctcctatcg
     3481 ccgggtcgag gcggtgcacg tggccctgga ccgcatgggc gaacggctca agcgcggcaa
     3541 cgcgctgctc ggcgccggcg cggagctgaa cgccaactat gccggcctgg aggccgattt
     3601 ccgcgcgttc ttccccgagg tggtgcgctt tgcgcgtgag gatctaggaa ctaggatcta
     3661 ggggctgggg gggtgttgtg cggcggagcc gcgccttttc cctagatccc agctcgcaga
     3721 tcctagcccc tcagtagaac cacccccagt gcatccccgc cggcggggtg tactgataat
     3781 cctcgccctg gtcctccacc cccgctgcgc cgccgctgat gccgagcagg ctcggctcgt
     3841 aagcgaagta gacgacgtcg ctgctgctgg agtacacgcg cgagtggatc gccggataga
     3901 tggcggcgga cggcggaatg gcgtagatgt cgggcgggat ggtgtcggcg agccgccacg
     3961 gaccggcgac cgtctcggcg ctgaaccagg cgccgttctg aatcgcgaac cagcgccggt
     4021 cgtcgagccg gatcaccggg accgacgcgt tggcgacgta gccgagctgc gtgccctcga
     4081 ccggctcgag ccggggatcg ccgtcgaggg tcaggtacag gctggtgcgc gtggggctgg
     4141 cggcgaccat caccggcgcg atgccgcggg cgggcgccgc gtgctcctgc ttcgccgccg
     4201 cgatgccggc cttgacgccg cgtttggggc tgtcgtcggg gatcgcggca aagctctccg
     4261 gcaggcggtg cgcggacacg aactcccagg gccccgcggt ggagccggag cggaaccagt
     4321 agcccgcgat gcgcacgtag agctgtttcg tctccacgtc gcggaacagg tgcgccgtgg
     4381 tgttggtcgc gtactgcagc gaagtgcccg ccacggcctc gaagcgcggc tcgccgtcga
     4441 gcaccagcag cgctgtgggc ttcgtggcca ccaggatctg cggcagcgat ccgctcttca
     4501 ggctcggccg acgccgcgtc ttcgggtcgg gcttgccctg caggaggttg acgcggccgc
     4561 tggcgcgcgc cacctgcgcg acctgggtgg acgccggcgg cggtttgctc acggaccagg
     4621 gcccggccag cgattcggcg cgggcccagc cgtcgtacag gcgcagatag tgccgtccct
     4681 tggcgtccct gagcagcagc gtgcgcgtgt tgagcacgcc gctcagttgc gtatccttca
     4741 ccggcacgta gcgcggctcg ccgtcgatgt acaccagcac ggtgggcttc ttggcaatca
     4801 ccacgcgcgg cgcgaccacg ctcagcacgg cggcgccgcg ctcgcgggcg cgcaccgccg
     4861 ggataccgga ctcgacgcgt tccagggcga cgccgttcac ctgctgcggc aggccggcac
     4921 gcagcgtctc gagccacgcc gcctgcgtct tgtccggcgc gccgggaaag cgcgccgccg
     4981 tgacctccag gtcctgcagg gcgaccgtcc cggcgccgtc gtcgacttcg gtgcgcgccg
     5041 tcatgcgcac cgcgccgatc gcgggctgct tgcgcccccc cggttgggcc ttgaccagtg
     5101 catgcgcgag cagcgcctcg ccgtcccagg cgtccacctg cggcgcatgg atctcgaaac
     5161 tcacgccgtt cgccgcgacg gtgtgcggcc actgcggggc cttcgcggcg gcggcggcgg
     5221 ccgccgccgg ctccggtgcg gcggcgacgt ccggcatgac cgtcggctcc gccgctgcga
     5281 gctcggctgc gacgttcgag gcggacatcg gctccgcgcc ggctgcgctt gccccgatgc
     5341 ttgccgcggc gctcgtatcg ggcgcgtgcg ggtcgggctc gacgtcggcg cctgcggaag
     5401 gcgcactcga tgcggcagca ctttcgcgcg gcgcggtcgc gtcctgcgcc ggctcgttgt
     5461 cgacggattg cggcggcgct tccgccgctg cggcctgagc ggacgcggca gcggcagtcg
     5521 catcggcggc ggcagcggcg gcggtcgctt cggcgtcggc cgttgcttcg gcggtcgaag
     5581 gcgatgcggc gctcggctgg ctcagcgtcg gcgggaacag ctcgcgcagc tcgccccagt
     5641 ccatgcgatc gcgcgcctcg cgcttgccgg cgcacgcggc aaggagaagc agcagcagca
     5701 gagcgaatcg cctcatgggt gtggcccggt ggagatagag tgcggcccgg tggagataga
     5761 gtgtggtccg gcagagacag tgcgcagcgc gcagcgcgaa gcggcagatg atagcggatc
     5821 gcgcgcggcc gtccacgcca tgcggtgcgg tctcaacgtg cgcgcggcgc gggctcgtcg
     5881 aggaacagct gcacttcgcg gaacagctgc aaccggttgg cttccatgat caccgtgtgc
     5941 gtgccggccc cgatcaccac caggcgtttc cacgcggcct tctccagcag cgcgaacacc
     6001 gactgcgcca tgtagcccgg cgtgtcctga tcccactcgc cgtggatcaa cagcgtgggc
     6061 gcggtgatgg cgcgcggatc gtagtaggga cggcccgccg accagtactc gcggccgtcc
     6121 cgcaccacgc cgttgggcgc gcgcaggagc ggcggattcc acttgccact gtccggatcg
     6181 gtggcccagg tggcctgcgc ccaggcctcg aaccagccct cggggatgag atcgtgcctg
     6241 gcggtatccg gcacgccggt gagccaacgc ttcctcgcct gctcctgcgt caccgtgcga
     6301 taggtcccga gcggaccgtc gccggccacc agcgaaggcg tctggcgcag ccacagcggc
     6361 gcatacagca ccagccgcgc caccttgtcc gggttgccgg ccgcataggt gcccatgatc
     6421 gccgtgcccc aggaccagcc gagcaggttc aagcgtgtca cgccgcggcg cctgaggacg
     6481 tgctccaccg ccgtgcccac gtcgcgcacc gccacgtcgg tggtgacgat gggctcgcct
     6541 tcctccgccg gtcgcgccat ctcggcgggc cgggtggact ggccgtagcc gcgcagatcc
     6601 accagataca cgtcccagcc gtggcaggcg atgtagtcca tccaggacat gcccgctagc
     6661 ggcaggtcga aggccgtctc cgcggggtag gtggcgccgt gcacgtacaa gagaacgcgc
     6721 tcggccgcga agctcttgac ctgcacggga cgcttgttgc gcaggtacag ctcgatcccc
     6781 ggcgtgtcgg aggcgatgcg atagctctcc gtggcgagct cgcgcgtgga gatgccggcg
     6841 gtctcgcata cgctcttggc ctgcgcgccc ccggcgaaag cgatcaacgc caggcatgcg
     6901 caaggcgcaa tcacacgagt catcgaggtc cctcctgtaa gatggccggc gaagcttacc
     6961 acccgcgcgc cgcaccgcag agccaaggat gtccagccac ccctacgacg tcctcacgcc
     7021 ggacctgatc ctggacgcgg tggagagcgc cggtctgcgc tgcgacgggc gattgctggc
     7081 gctcaacagc ttcgagaacc gcgtctacca ggtgggcatc gaggaggcgc agccggtaat
     7141 cgtcaagttc taccgccccg ggcgctggag cgacgcgcag atcgccgagg agcacgactt
     7201 cgcgcgcgag ctggcgcagg cggagcttcc cgtcgtggcg ccgctggccg tggacgaggg
     7261 cgggggcgag ggcggcggcg aggccggtgg cgagagcggc gcgctgcgaa cgttcggcgg
     7321 ctttcgcttc gcgctgtatc cgcgccagcg cgggcgcgcg ccggagctcg aatcgcagga
     7381 cacactgacc tggatggggc gcttcatcgg ccgcatccac gcggtcggcg cgctgcggcc
     7441 gttcgcgcag cgtccggcgc tgaccatcga gagcttcggc gaggagcccg cgcgcttcct
     7501 gctggacagc ggcttcgttc cggccgacct cgccctcgcc tatcgcacgc tggccgagca
     7561 gctgctcgct acgatccgcg cctgcttcac gcgcgccggg cagagcgctg cggtccgcct
     7621 gcacggggac tgtcatccgg gcaatgtgct gtgggccgac gacgggccgc acttcgtgga
     7681 cctggacgac gcgcgcatgg gtccggcggt gcaggacctg tggatgctgc tgtccggcga
     7741 tcgcgacagc atggcgcgcc agctgcgctg gctgctggac ggctactggg agttccacga
     7801 gttcgagctg cgcgagctgc agctgatcga gccgctgcgc acgctgcgca tgatgcacta
     7861 tgcggggtgg ctggcgcggc gctgggacga tccggccttt cccgccgcct tcccctggtt
     7921 caacacgcag cgctactggc aggaccacat cctggccttg cgcgagcagg cggcggcgat
     7981 ggacgaggcg ccgccggcgc ctgcccccgg gtaggcgttt cctggcccgc aatttgcagc
     8041 gctgggcggt tgcgaccctc ccgaacagcg agaaaccgga cgtgaacgcg ccgcatcgaa
     8101 tcgtcgcccc gtggggcctc aagcaagtgg tgccgctgct caagacccac cgcgtgcagc
     8161 tcaccgagcc gggcatggcg cccgcattca gcctgaacct gcacgcggtg ccgctgggcg
     8221 cctcggagtt cggcgtggcg atgcgcgact acccgatcgt gttcctgcgc cgcgcacgcg
     8281 acggcgtgca ccaggcggtg gcgatcctgg gcatgaagga aggggaaaac ctgtacgtca
     8341 tgcccgacgg ccgctgggac cggcgcacct acatcccggc ctatatccgg cgttatccgt
     8401 tccatgtcga ggtcaccggc gccgagcgca ccatctgcgt cgatccggat gccgtggcgg
     8461 acgatggcga gcgcctgttc gacgaggtcg gcgcgccgct gcagcactgg atcgtgttcg
     8521 agcgcctgct caacgagtac gaagacgagc tggcgcgcgc ccatcgcctc ggcgacaagc
     8581 tgcacgcgct gggcgtgctg gcgccgttct cgctgcacgc cgatctgtcc agcgggttct
     8641 cgctgtcgct ggacggcatg ttccgggtgg accgcgagcg cctcggcgcg ctcaaggcgg
     8701 ccgcgctgcg cgagctgatc ggctcgggcg cgatggagtc ggtgtacgcg cacctgctgt
     8761 cgcaggagaa cttccgccgg ctgctgtcgc gccgcggttt cttcgcgaag cggccggctt
     8821 cctgagccgg gcgcctgagc ggggccgcgc ttcgggcggc gacgcggtgg cgcttgacgc
     8881 tgcatgacgc cctgattaca gtttgctgcg ccggcaagcc gatggttatc caacaagcac
     8941 aaccaagccg acgtccaccg ggcgcgctgc cgcgtcgctc gtgcgggcct ggcccgaccc
     9001 gaggcgagtg acatgctcta cgaactgcac gagctgcaga agaccgtgtc ggcgcctctg
     9061 ctggccttgg tccacgccag ccaccgcctg ttcagcaacc cctacagtcc gctcgcgtac
     9121 acgccggtca gccgccacat cgccgccagc agcgagctga tcacgcggct gatgcgctcc
     9181 tatcacaagc ccgcgtgggg catcgaccac gcgctggtcg cggccgaacc gcctgcagag
     9241 aagcgccctg gggaaacgcc cgccttgcct ggacaaacac tgggcgtgcc ggtgctgatc
     9301 gagccggtgc tgaccaggcc gttctgccac ctgctgcact tccgcaagca gaccgaccga
     9361 cccgggccga aggtgctgat cttcgcgccg ctgtccggtc atcacgccac tttgctgcgc
     9421 gataccgtgc gcaccatgct gcgcgacttc gacgtgtacc tcaccgactg ggtggatgcg
     9481 cgcatggtgc cgctcgccag ggggccgttc cacctggagg actacgtcga ctattgccag
     9541 gagttcatcc gctttctagg cccgggcatc gccaccgtgt cggtgtgcca gcccacggtg
     9601 ccggtgctgg cggcgatcgc gctgctggcg caggagcagg acgcctgcgt gccgcgcgcg
     9661 atggtcatga tgggcgggcc gatcgacacg cgccgcagcc ccacggtggt caacgagctg
     9721 gcgatgggcc ggccgcactc gtggttcgag gaccaggtga tccaccgcgt gccgttcaac
     9781 tacccgggct tcatgcgccg cgtgtatccc ggcttcctgc agcacctgtc gttcatcgcc
     9841 atgaacgccg accgccacat cgaggcgcac cggcggttct tcaatcacct gatcgtgggc
     9901 gacggcgaca gcgcggaatc gcaccgccgc ttctacgacg agtacaacgc ggtgctcgac
     9961 atgcccgcgg agtattacct ggacaccatc aaggtggtgt tccaggactt cctgctggcc
    10021 aagggccgct gggaggtgcg cggcgccctg gtgcggccgg aggcgatccg caacgccgcg
    10081 ctgttcaccg tggaaggcga gctcgacgac atctccggca acggtcagac cgaggccgcg
    10141 cacggcctgt gccgcaacat cgctgcggag aagcgccggc acctgttggc ccagggcgtg
    10201 ggccactacg gcatcttcag cgggcgcaag ttccgcgaca ccatctaccc gcagatccgc
    10261 gatttcatcc ggcgctggac agcagtctga tccggcccgc ggccctgtag aatctcctcc
    10321 ttcggcgccg gcgctcggct gggcggcatg ttcgaggaga acttcgccaa ctcgctgatc
    10381 aaggccgaag gcaactggct ggcgttcttc gagcggccga tcgccggcac gctgggggtg
    10441 gtcacgttgc tggtgtggtt atcgccgctg gccttgggtg ggtggaggcg gcggcgcgct
    10501 ccggcctgac ccccgttaca attgaaccgt ccggcacgcg ccacggtcaa acggcgtgca
    10561 gcgccccgca ggtgcccggg cgccggtcaa acattcgccg tatcgctaat tcgccgagtc
    10621 gcgcattcgc cgagtcgtac agtcgccgag tcgtacattc tctgggggag gtcctgatgg
    10681 caacgctcaa agtgaacgga aagcaggtga gaaaggacgt ggagcccgac atgccgctgc
    10741 tgtggctgct gcgcgacgag ctgggcctta ccgggacgaa gtacgcctgc ggcatcgccg
    10801 cctgcggcgc ctgtaccgtg cacgtggacg ggcagccggt gcgcgcctgc gtgatgccgg
    10861 tctcggcggt ggagggcaag gagatcacca ccatcgaggg cctgtccaag gacggcagcc
    10921 acccggtgca gaaggcctgg gtccagctgc aggtgcccca gtgcggttat tgccagagcg
    10981 gcatgatcat ggccgcggcc gcgctgctga aggcgaatcg caagccctcg gacgcggaca
    11041 tcgacgccgc gatgaccaac ctgtgccggt gcggaacgta ccaccgggtg cgccaggccg
    11101 tccacctggc cgcgaagtcc gcgtaacggg aggccgacat gacatatctc atcgatagca
    11161 accgtcgcga gttcctggtc aagtccgccg ccgtggccgg cggcatgatg ctgggcgtgc
    11221 gtctgcccgg cgccgcgcag gcggccgagg gcgcggcagg gccggaggtc acccactgga
    11281 tcgtgattca cccggacgac accgtggtgg tgcgcatcgc ccgctcagag ctgggccagg
    11341 gcacgttcac cgggctggcc atgctggtcg ccgaggagct ggaggccgac tggagcaagg
    11401 tgcgcgccga atatgcggac gtgaacgagc acgtgcgccg caaccgcatc tgggg
//
LOCUS       DASPGE010000068         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1075916, whole genome shotgun sequence.
ACCESSION   DASPGE010000068 DASPGE010000000
VERSION     DASPGE010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075916"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VD791_02165"
     CDS             complement(<1..351)
                     /locus_tag="VD791_02165"
                     /inference="COORDINATES: protein motif:HMM:NF036723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06805.1"
                     /translation="MPFNQPAPRIGNQYGEDRVLREYLARVLPAEVREALQSGLQEMG
                     ELAGGPLYAFQLADRAAEPTHTPWDAWGNRIDQIEVSPLWRAAEPLAARSGLIAIPYE
                     RAHGQWSRVHQFALV"
     gene            499..1884
                     /gene="fumC"
                     /locus_tag="VD791_02170"
     CDS             499..1884
                     /gene="fumC"
                     /locus_tag="VD791_02170"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HXV06806.1"
                     /translation="MCAAPRIEHDSMGDVPVPAERLWGAQTQRALMHFAISTERMPGE
                     LVRALVLVKRAAATVNARLGALPADKADAIVRAADEVLAGQHEAEFPLSVWQTGSGTQ
                     TNMNVNEVLANRASELLGGPRGPGRLVHPNDDVNRSQSSNDAIPTAMHVAAVTALTDR
                     LLPSLLRLKTTLAHKAEAFQGIIKVGRTHLQDATPLTLEQEFSAYVAQLDQAQLAIEG
                     AVGELYALAIGGTAVGTGLNAPPGFDAAMSEEIARVTGLPFFPAPNKFAALAAHEPLV
                     RLHGCLKALAVALTKIANDVRWLGSGPRAGLGEIRLPENEPGSSIMPGKVNPTQAEAL
                     LMVCAQVMGNDVAVGIAGASGNFELNVSKPLLIHAVLQSVRLLGDAMRSFNQHCAEGI
                     EPNEARIAELLARSLMLATALAPHIGYDRAAEIARKAHREGSTLKEAALALGYVSERD
                     FDAWTDPAGMV"
BASE COUNT          305 a          704 c          711 g          290 t
ORIGIN      
        1 taccagcgcg aactggtgca cgcgcgacca ctggccgtgc gcgcgttcgt agggaatggc
       61 gatcaggccg ctgcgcgcag ccagcggttc ggcggcgcgc cacagcggcg acacttcgat
      121 ctgatcgatc cgattgcccc aggcgtccca gggcgtgtgc gtcggctcgg ctgcgcggtc
      181 ggcgagctgg aatgcgtaca gcggcccgcc ggcgagctcg cccatctcct ggaggccgct
      241 ctggagcgcc tcgcgcacct cggccggcag aacacgcgcc aggtactcgc gcagcacgcg
      301 gtcttcgccg tattgattgc cgatgcgtgg cgcgggctgg ttgaagggca tgcggcacta
      361 taccgcgctc gccgtccgat cgcccgtccc gtttcgtgga gcggccgtgg catcgggtcc
      421 agcgcgtgca ggccagcgcg gccgggttgg gtgtagcatt cctcaccacc accatcctgt
      481 cgcttgcccc aatcgtccat gtgcgccgcg ccccgaatcg agcatgacag catgggagat
      541 gtgccggtgc cggccgaacg cctgtggggc gcgcagacgc agcgcgcgct gatgcacttc
      601 gccatctcca ccgagcgcat gccgggcgag ctggtgcgcg cgctggtcct ggtcaaacgc
      661 gcggccgcga cggtcaacgc gcggctcggt gcgttgcccg cagacaaggc cgacgcgatc
      721 gtgcgcgccg cggacgaggt gctcgccggt cagcacgagg ccgagtttcc gctgtcggtg
      781 tggcagaccg gctcggggac gcagaccaac atgaacgtca acgaagtgct ggccaaccgc
      841 gcctcggagc tgctcggcgg cccgcgcggg ccgggccggc tggtgcaccc caacgacgac
      901 gtcaaccgca gccagtcctc caacgacgcg atccccaccg ccatgcacgt ggccgcggtc
      961 accgccctca ccgaccggct gctgccgtcg ctgctgcgcc tgaagaccac gctcgcgcac
     1021 aaggccgaag cattccaggg catcatcaag gtgggccgca cccatctgca ggacgccacc
     1081 ccgctcacgc tcgagcagga attctccgct tacgtggcgc agctcgacca ggcgcagctc
     1141 gcgatcgaag gcgcggtggg tgagttgtat gcgctggcga tcggcggcac ggcggtgggc
     1201 accggcctga acgccccgcc gggattcgac gcagcgatga gcgaggagat cgcgcgcgtg
     1261 acggggctgc cattcttccc cgccccgaac aagttcgccg ccctggccgc gcacgagccg
     1321 ctggtgcgcc tgcacggctg cctcaaggct ttggcagtgg cgctgaccaa gattgccaac
     1381 gacgtgcgct ggctaggcag cggcccgcgc gccggcttgg gcgagatccg cctgcccgag
     1441 aacgagccgg gcagctcgat catgccgggc aaggtcaatc cgacgcaggc cgaggccctg
     1501 ctgatggttt gcgcccaggt gatgggcaac gacgtggccg tgggtatagc gggcgcgtcg
     1561 ggcaatttcg aactgaacgt gagcaagccg ctgctgatcc acgccgtgct gcagagcgtg
     1621 cggctgctgg gcgatgcgat gcgctccttc aaccagcact gcgccgaggg gatcgagccg
     1681 aatgaggcgc gcatcgcgga gctgctcgcg cgctcgctga tgctcgccac cgcgctggcg
     1741 ccgcacatcg gttacgaccg cgccgccgag atcgcgcgca aggcgcaccg cgagggcagc
     1801 acgctgaagg aagcggcgct cgctctgggt tacgtcagcg agcgcgactt cgacgcctgg
     1861 accgatccgg cggggatggt gtgagaatcc cgcaccccgc ctctcacgag gcggggtggc
     1921 gccgtcaggc gccagggcgg tgtggtgtga gctggccgga ccaccccgtc acgcttcgcg
     1981 tgccacccct cctcgacgag gaggggaatc
//
LOCUS       DASPGE010000069         3619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1544061, whole genome shotgun sequence.
ACCESSION   DASPGE010000069 DASPGE010000000
VERSION     DASPGE010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544061"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1155)
                     /gene="paaE"
                     /locus_tag="VD791_02175"
     CDS             complement(88..1155)
                     /gene="paaE"
                     /locus_tag="VD791_02175"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HXV06807.1"
                     /translation="MTPRFHSLRIAEVRRECADAVSLRFEVPPRLAADYAFVQGQHVT
                     LRTRLGGRELRRSYSICAAADDGELRVAIRKVAGGAFSTFANAQLKDGDAIDVLTPEG
                     RFHTPLDPANAKHYVAFAAGAGITPVLSLIKTTLKREPRSRFTLLYGNRRVATTMFQE
                     ELEDLKDRYLERFTLYNLFSREHQEIELFNGRLDAAKVQALADTLLPVAGIDEAFLCG
                     PDTMVDEIEAALRARGLSAAHIHLERFGVPAAGAAHHVEPGDAPQALITVLVDGVRRE
                     VEFHAGDPSVLDAALRAGLDLPYSCKGGMCCTCRAKVLEGRLRMDKNYSLEQRDLDAG
                     FVLTCQSHPLTERVVISYDER"
     gene            complement(1160..1642)
                     /gene="paaD"
                     /locus_tag="VD791_02180"
     CDS             complement(1160..1642)
                     /gene="paaD"
                     /locus_tag="VD791_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HXV06808.1"
                     /translation="MLSIAQAWQVLARVPDPEIPVISVTELGIVRDVRTEGEALTVVV
                     TPTYSGCPATEAIETAIRDALREAGAGEVRIETRLAPPWTTDWLDPRAADRLREYGIA
                     PPGSAASVHSVRWAPRPPACPRCGSHDTERLSEFGATACKALYRCAACREPFEHFKPI
                     "
     gene            complement(1650..2402)
                     /gene="paaC"
                     /locus_tag="VD791_02185"
     CDS             complement(1650..2402)
                     /gene="paaC"
                     /locus_tag="VD791_02185"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HXV06809.1"
                     /translation="MQDLHIEYLLRLADSPLILGQRLSEWCGHAPVLEEDLALANTAL
                     DLIGQARLLLTHAGAQEGSGRDEDQLAFLRSEREFRNCTLAELPNGDFARTVLRNFLF
                     GAYQVPLWEGLLGSRDAQLAAIAAKSLKEARYHLRHAADWAVRLGGGTEESHCRMQAA
                     LDYLWPYTAELFAGDAVEEAAAAAGVGVTGSSLQARWEATVLPVLQEAGLSPPARTPF
                     LSQGKLGVHSEHMGHLLAEMQTMQRAYPGAQW"
     gene            complement(2426..2713)
                     /gene="paaB"
                     /locus_tag="VD791_02190"
     CDS             complement(2426..2713)
                     /gene="paaB"
                     /locus_tag="VD791_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HXV06810.1"
                     /translation="MDRNEWPLWEVFIRSRNGLDHKHCGSLHAPDARMALQNARDIYT
                     RRMEGVSIWVVRSDQIHASDPEAKPEMFDPAEDKIYRHPTFYKLPQEVDHM"
     gene            complement(2724..>3619)
                     /gene="paaA"
                     /locus_tag="VD791_02195"
     CDS             complement(2724..>3619)
                     /gene="paaA"
                     /locus_tag="VD791_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HXV06811.1"
                     /translation="IDAGEFIEAKDWMPEHYRKTLVRQISQHAHSEIVGMLPEGNWIT
                     RAPSLKRKAILLAKVQDEGGHGLYLYAAAETLGVSRDALVEALHSGKAKYSSIFNYPT
                     LSWADIGAIGWLVDGAAIMNQIPLTRCSYGPYARAMIRICKEESFHQRQGFDLLLTMM
                     RGTPQQRAMVQDAVNRWWWPVLMMFGPHDSASTHGEQSARWGIKRISNDDLRQKFIDA
                     TVPQAQVLGVTLPDPQLRWNQARGHWDFGPIDWQEFWNVVNGHGPCARERIAARVKAH
                     EDGAWVREAALAYARKQAAAA"
BASE COUNT          518 a         1259 c         1266 g          576 t
ORIGIN      
        1 ggggccccat cgcttgcgat ggggatgcga ccgcggaggc gcatgacatc cgcgcggtcc
       61 ggccaaagcc tacaactctc cacgcgctca tctttcgtcg taactgatga ccactcgctc
      121 ggtaagcgga tgcgactggc aagtgagcac gaaaccggcg tcgagatcgc gctgctccag
      181 gctgtagttc ttgtccatgc gcagccgccc ttccagcact ttggcgcggc aggtgcagca
      241 catgccgccc ttgcacgaat agggcaggtc gagaccggcg cgcagcgcgg cgtcgagcac
      301 cgaggggtcg ccggcgtgga actccacctc gcgccgcacg ccgtccacca gcaccgtgat
      361 cagcgcctgg ggcgcatcgc ccggctcgac gtggtgcgcg gcgccggcgg cgggcacgcc
      421 gaagcgctcg aggtggatgt gcgcggcgga caggccgcgc gcgcgcagcg cggcctcgat
      481 ctcgtccacc atcgtgtccg ggccgcagag gaacgcttcg tcgatgccgg ccaccggcag
      541 cagcgtgtcg gcgagcgcct gcaccttggc tgcgtccagc cggccgttga acagctcgat
      601 ctcctgatgc tcgcggctga acaggttgta gagggtgaag cgctcgaggt accggtcctt
      661 gagatcctcg agctcctcct ggaacatggt ggtcgccacg cgccggttgc cgtacagcag
      721 cgtgaagcgg ctgcgcggct cgcgcttgag cgtggtcttg atcagcgaga gcaccggcgt
      781 gatgccggcg cccgcggcga aggcgacgta gtgcttggcg ttggccggat cgagcggcgt
      841 gtgaaagcgg ccctcgggcg tgagcacgtc gatcgcgtcg ccgtccttga gctgcgcgtt
      901 ggcgaacgtc gagaacgcgc cgccggccac cttgcggatc gccacgcgca gctcgccgtc
      961 gtcggccgcc gcacagatgg agtacgagcg gcgcagctcc cggcccccga gccgcgtgcg
     1021 cagcgtcacg tgctggccct ggacgaaggc gtagtcggcg gccagccgcg gcggcacttc
     1081 gaagcgcagg cttaccgcgt ccgcgcattc gcggcgcacc tcggcgatgc gcaggctgtg
     1141 gaagcggggc gtcacgcgat cagatcggct tgaagtgctc gaacggctcg cggcatgcgg
     1201 cgcagcgata cagcgccttg caggccgtcg ccccgaactc cgacaggcgt tcggtgtcgt
     1261 gggaaccgca gcgcgggcac gccggcggtc gcggcgccca gcgcacgctg tgcaccgagg
     1321 ccgcggagcc tggcggtgca atgccgtact cgcgcagcct gtcggcggcg cgcgggtcga
     1381 gccagtcggt ggtccagggc ggcgcgagcc gggtctcgat gcgaacctcg cccgcacccg
     1441 cctcgcgcag cgcatcgcgg atcgccgtct cgatcgcctc ggtggcgggg cagccggaat
     1501 aggtgggggt gaccactacg gtcagcgcct cgccttcggt cctgacgtcg cgcacgattc
     1561 ccaactcggt gaccgagatc accgggatct cggggtcggg cacccgggcc aggacttgcc
     1621 aggcctgtgc gatgctcagc acgcgtgcct caccattgcg cgcccgggta ggcgcgttgc
     1681 atggtctgca tctcggccag caggtgcccc atgtgctcgc tgtgcacgcc gagcttgccc
     1741 tgcgacagaa agggtgtgcg cgcgggcgga gacagccccg cctcttgcag caccggcagc
     1801 accgtcgcct cccagcgcgc ctgcagcgag ctgcccgtca cgcctacccc ggcggcggcg
     1861 gccgcttcct cgaccgcatc gccggcgaac agctcggcgg tgtaaggcca cagatagtcc
     1921 agggcggctt gcatgcggca gtgggattcc tcggtgccgc caccgaggcg aaccgcccag
     1981 tcggccgcgt gccgcaggtg atagcgcgcc tccttgaggc tcttcgcggc gatggcggcg
     2041 agctgagcgt cgcgcgaacc gagcaggcct tcccacagcg ggacctgata ggcgccgaac
     2101 aggaagttgc gcagcaccgt gcgggcgaag tcgccgttgg gcagctccgc cagcgtgcag
     2161 ttgcggaact cgcgctcgga gcgcaggaac gcgagctggt cctcgtcgcg accgctgcct
     2221 tcctgcgcgc ccgcatgggt gagcagcagg cgcgcctgcc cgatgagatc cagcgcggtg
     2281 ttggccagcg ccaagtcctc ctccaggacc ggcgcgtgcc cgcaccactc cgacagccgc
     2341 tggcccagga tcagcggact gtcggcgaga cgcagcagat attcgatgtg cagatcctgc
     2401 atcgctccgg cggccgcggc gcggctcaca tgtgatccac ttcttgcgga agcttgtaga
     2461 aagtcggatg ccggtagatc ttgtcttcgg cgggatcgaa catctccggc ttggcctccg
     2521 gatcggaggc gtggatctga tcgctgcgca ccacccagat gctcactccc tccatgcgcc
     2581 gcgtgtagat atcgcgcgcg ttctgcagcg ccatgcgtgc gtcgggcgcg tgcaggctgc
     2641 cgcagtgctt gtggtccagg ccgttgcggc tgcggatgaa cacctcccac agcggccatt
     2701 cgttgcgatc catcgaagtt ctcctatgcc gcggccgctt gcttgcgcgc gtaggccagc
     2761 gccgcctcgc gcacccacgc gccgtcctcg tgcgccttga cgcgcgcggc gatgcgctcg
     2821 cgcgcgcacg gcccgtggcc gttgaccacg ttccagaact cctgccagtc gatcggcccg
     2881 aaatcccagt gcccgcgcgc ctggttccag cgcagctgcg gatcgggcag cgtcaccccc
     2941 agcacctggg cctgcggcac ggtggcgtcg atgaacttct ggcgcaggtc gtcgttggag
     3001 atgcgcttga tgccccagcg cgcgctctgc tcgccgtggg tggaggcgct gtcgtgcggc
     3061 ccgaacatca tcagcaccgg ccaccaccag cggttcaccg cgtcctgcac catggcgcgc
     3121 tgctgcggcg tgccgcgcat catggtgagc agcaggtcga agccctggcg ctggtggaag
     3181 ctctcctcct tgcagatgcg gatcatggcg cgcgcgtagg gcccgtagga gcagcgcgtc
     3241 agcgggatct ggttcatgat cgccgcgccg tccaccagcc agccgatcgc gccgatgtcg
     3301 gcccaggaaa gggtcgggta gttgaagatg gaagagtact tggccttgcc gctgtgcagc
     3361 gcctcgacca gcgcgtcgcg cgacacgccc agcgtctcgg cggcggcgta caggtacagc
     3421 ccgtgcccgc cttcgtcctg caccttggcg agcaggatgg ccttgcgctt gaggctgggc
     3481 gcacgcgtga tccagttgcc ctcgggcagc atgccgacga tctccgagtg cgcatgctgc
     3541 gagatctggc gcaccagcgt cttgcggtag tgctcgggca tccagtcctt ggcctcgatg
     3601 aactcgcccg cgtcgatgc
//
LOCUS       DASPGE010000070         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1075969, whole genome shotgun sequence.
ACCESSION   DASPGE010000070 DASPGE010000000
VERSION     DASPGE010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075969"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1708
                     /locus_tag="VD791_02200"
     CDS             <1..1708
                     /locus_tag="VD791_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV06812.1"
                     /translation="PRASGQRHRVLGRVGLQLLLLTPMRNAAAGSVKAAEAISVSAWS
                     PFRNGIFAMLWTATVVSNIGTWMQDVAAGWLMTSLAPSPAMVALVQAATTLPVFLFAL
                     PAGALADLMDRRKLLLVTQSSLLVVTASLGVLTQLDLVTPVVLLVFTFLAGLGAAMTA
                     PAWQAIVPELVPRPALQSAIALNSLGINISRAIGPALGGFLIAAFGVAVPFFVNAVTF
                     VGVLAALFVWKRQAVHSTLPGERFLGAIRTGLRYARESAPLRATLVRSAAFFVFASCY
                     WSLLPLIARHELRGGPGLYGMLLTAIGLGAVAGALALPVLRKKISSDHLVVGGTAVTA
                     AIMAALALAPRVEVALVAFLCAGGAWIAVLSSLNVSAQTALPNWVRARGLSVYLMVFF
                     GSMALGSVIWGQTATYTGLQTALLIAAGGAVAAILVTRRWGVQSGGLDLAPSMHWPAP
                     AVADAIAPDRGPVMITVEYRIDQAQAAAFVARLTELSHSRRRDGAFAWGVFEDAEKPG
                     RYLEYFLVESWAEHLRQHERVTVADRDLQEGVQNFHLGPGKPIVSHFLAPAAIPTNGR
                     ET"
     gene            1705..1929
                     /locus_tag="VD791_02205"
     CDS             1705..1929
                     /locus_tag="VD791_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010396484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HXV06813.1"
                     /translation="MKIALGLLLGFLIGALCRFADIPVPAPPALVGALLVLAMTTGYV
                     LTDRWASHRAAHHRELCGGPNGRPITRSDA"
     gene            1926..2135
                     /locus_tag="VD791_02210"
     CDS             1926..2135
                     /locus_tag="VD791_02210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HXV06814.1"
                     /translation="MTAALIGLALGLFIGAGCRWFDIPLPAPPRLVGALLVVAMTLGS
                     VATDLTLASRAPATSHVPVSTSTAG"
     gene            2137..2550
                     /locus_tag="VD791_02215"
     CDS             2137..2550
                     /locus_tag="VD791_02215"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXV06815.1"
                     /translation="MDGAASLAARICLSAVFLYSGFTKLLDLPAGYAEVAGLGLPFPW
                     IALAATLVVQIGGGLTVLIGWRALFGAFLLAAFTLIATLLAHGFWSPSGVERIRQLTT
                     SLEHLAIVGGFILIMAYGPGPFSFEARRGRADRAA"
     gene            2547..>2859
                     /locus_tag="VD791_02220"
     CDS             2547..>2859
                     /locus_tag="VD791_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06816.1"
                     /translation="MIARLAGAVLAGATILASVAAAAQDRPSFKRLRYDEDWSTMCEP
                     EMRTEPLDAIKCIKLIDSGESWLSLGGELRERYEYTHNPTWGNDPQDKHGVWLQRYVL
                     HA"
BASE COUNT          371 a          961 c          975 g          552 t
ORIGIN      
        1 cccgcgtgcc agcggccaac gccaccgcgt tctggggcgc gttgggctgc agctgcttct
       61 tttgacgccg atgcgtaacg cggcggccgg ttcagtcaaa gcagccgagg cgatttccgt
      121 ctcggcctgg agcccattcc gtaacggcat cttcgcgatg ctgtggaccg cgacggtggt
      181 ctccaatatc ggcacctgga tgcaggatgt cgccgccggc tggctgatga cgtcgctcgc
      241 accctcgccg gccatggtgg cgctcgtcca ggcggcaacg acactgccgg tcttcctctt
      301 cgcgctgccg gccggggcgc tcgccgatct catggaccgg cgcaagctgc tgcttgtcac
      361 gcaaagctcg ctgcttgtgg tcacggcatc gctgggcgtg ctgacgcagc tcgatctcgt
      421 gacacccgtg gtgctactcg tgttcacgtt cctggccggg cttggtgccg cgatgacggc
      481 gccggcttgg caggcgatcg tgccggagct ggtgccgcgg ccggccctac aatcggcgat
      541 cgcgcttaac agccttggca tcaatatctc gcgggcgatc gggccggcgc tgggcggatt
      601 cctcatcgca gcgttcggcg tcgccgtgcc attcttcgtc aacgccgtca ccttcgtcgg
      661 cgtgctggct gctttgttcg tgtggaagcg gcaagcggtg cacagcacgc tgccgggcga
      721 gcgtttcctc ggcgcgatcc gcaccggctt gcgctatgcg cgcgaaagcg cccccttgcg
      781 cgcgacgctg gtgcgttccg ccgcgttctt tgtgttcgcc agttgctact ggtcgctgct
      841 gccgctgatc gcgcgccacg agttgcgcgg cgggcctggg ctctacggta tgctgctgac
      901 ggcaatcggg ctgggcgcgg tcgccggcgc gctcgccttg ccggtgctgc gcaagaagat
      961 cagcagcgat catctggtcg tcggcggtac ggccgtcact gccgcgatca tggcggcact
     1021 ggcgctggcg ccgcgtgtcg aggtggcgct ggtagcgttc ctctgcgccg gcggcgcgtg
     1081 gatcgcggtg ctgtcgtcgc tgaacgtttc ggcgcagacc gctttgccga actgggtgcg
     1141 ggcgcgcggg ctgtcggtct atctcatggt gttcttcggc agcatggcgc tgggcagcgt
     1201 gatctggggc cagaccgcga cctataccgg ccttcagacc gcgctgctga tcgctgctgg
     1261 cggcgccgtg gctgcgatcc tcgtgacgcg acgctggggc gtgcagagcg gcgggctcga
     1321 tctcgcgccc tccatgcatt ggcccgcacc agctgttgcc gacgcgatcg cgcccgatcg
     1381 cgggccggtc atgatcacgg tcgagtaccg catcgatcag gcccaggctg ccgccttcgt
     1441 ggcgcggctc acggagctca gccacagccg gcggcgcgac ggcgccttcg cctggggcgt
     1501 gttcgaggat gcagagaagc ccgggcggta cctcgaatat ttcctggtcg aatcctgggc
     1561 cgagcatctg cgccagcacg aacgcgtcac agtcgctgac cgcgatctgc aggaaggcgt
     1621 gcagaatttc catctcgggc ccggcaagcc gatcgtctcc cacttcctcg cgccggcggc
     1681 gatcccaacg aacgggagag agacatgaaa atcgccttgg gcctcctgct tggctttctc
     1741 atcggcgcac tctgccggtt cgccgacatc cccgtgccgg cgccgccggc cctcgtgggc
     1801 gcgcttctcg tgctggcgat gaccaccggc tatgtgctca cggatcgctg ggcgagccat
     1861 cgtgccgcgc atcaccgcga actctgcggc ggccccaacg gccgccccat cacgaggagt
     1921 gacgcatgac cgccgccctg atcggcttgg cgctcggcct cttcatcggc gcgggctgcc
     1981 gttggttcga tattccgctg ccggcaccgc cgcggctggt gggtgccctg ctggtcgtgg
     2041 cgatgacgct aggctccgta gcgaccgatt tgacgctggc gtcgcgcgcg ccggcgacga
     2101 gccacgttcc ggtcagcaca tccacggcag gttgagatgg acggcgccgc atctctcgct
     2161 gctcggattt gtttgagcgc ggtgttcctc tacagcggat tcactaagtt gctcgacttg
     2221 ccggctggct acgcggaggt cgcggggctc ggtctcccgt tcccgtggat tgcgctcgca
     2281 gccacgctcg tagtgcagat tgggggcggg ttgacggtgc tcatcggctg gcgggccctc
     2341 ttcggcgctt tcctactggc cgcgttcacg ctcatcgcga cgctcttggc gcatggcttc
     2401 tggtcgccgt ccggcgtcga gcggatccgc cagctcacga cctcacttga acatctggcg
     2461 atcgtgggcg ggttcatcct gatcatggct tacgggcctg gacccttcag cttcgaggcg
     2521 cggcggggcc gtgcagaccg tgcagcatga tcgcgaggct tgcgggggcg gtgctagccg
     2581 gcgctacgat cctcgcgagc gtggctgccg ccgcgcagga ccgacccagc ttcaagcgcc
     2641 tgcgctatga cgaagactgg tcgacgatgt gcgagccgga aatgcgcacc gaaccgctcg
     2701 atgccatcaa atgcattaag ctgattgact ccggtgagtc ctggctctcg ctcggcggcg
     2761 agctgcgcga gcgctacgag tatacgcaca accccacctg gggtaacgac ccgcaggaca
     2821 agcacggcgt ctggctccag cgctacgtgc tgcatgccg
//
LOCUS       DASPGE010000071         4990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_22187, whole genome shotgun sequence.
ACCESSION   DASPGE010000071 DASPGE010000000
VERSION     DASPGE010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22187"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VD791_02225"
     CDS             complement(<1..766)
                     /locus_tag="VD791_02225"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXV06817.1"
                     /translation="MAEQRKKLRLGELLVQQGLITPDQLRIALTEQKNQSVPLGRLLV
                     RLGFVTEAVIRDIMARTIGQESIDLTQVVVDGEAVRLIPQDFARRHRLLPIAFDGAAQ
                     ALTVAITEVFNMVALDQLRATIGQQIKIKTVLAGDAQLEDHIDQFYGHELSVDGILRE
                     IETGEIDYQSLQGGGDEYQQPIVRLVGALLVDAVKRGASDIHFEPEFAFLRVRYRIDG
                     VLEQVRSLHKNYWPGIAVRLKVISGMNIAETRAPQDG"
     gene            complement(777..2351)
                     /locus_tag="VD791_02230"
     CDS             complement(777..2351)
                     /locus_tag="VD791_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV06818.1"
                     /translation="MSLLLQALQKAAKNRESGGPEQEPERDALPYEREPHEAGPYEPG
                     PYEPGSHEPGAPESGLHEPGPREPELGELPADPDALSLTDDDDELFDPEPEDEPALTA
                     APEPTIAPARPPERPVLRPATAAGGSAHAATILRANEARSTGALDWVRDRPVHAFAIA
                     AAIFGVFYGAYVYLQIFHPAVLRGDFLTSFLQSKPPSAAKRRTPPPHAGRIEQAQPAQ
                     PAAAAVASSASAAPSTPAGTLSTPAGTLSTPPGTLSTPARAAAVPPAEAGAGEAAERP
                     FTGMPQAKASRRTAERPVASAAREKPPPRRATVEVEAAATLDDSVAVRAPPASATPPS
                     VPLMQAWEALNQGRFEEARALYQEVLRIEPHNVDALLGLAAIDTRAGNGEEAALHYAR
                     ALELEPRNSTAQAGLISLLGQADPLQSETRLKQLIARGPTGFLYFALGNLYARQGRWP
                     LAQPAYFQAYQLQPDNPDYAYNLAVGLEHLGQAKIALNYYRRALELSSLRGHASFEQA
                     RVQERVGQLSARVGSE"
     gene            complement(2348..4102)
                     /locus_tag="VD791_02235"
     CDS             complement(2348..4102)
                     /locus_tag="VD791_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06819.1"
                     /translation="MSRAAATRIPRLPGALLAGAALLASGACTTYRPTVPPSDAHLSA
                     DTVTPIEETRRILPPLTNSPFVPPPRPRTKPTTYSVVVHEVPVKELLLALARDTKENI
                     DIHPGLQGLVSLNAIDETLPSILERISRQVNMRYRVEGRTIVVSPDTPYLKTYRVNYV
                     NMVRDTDSTIGVTGQVGTASDLTNQAVGASQTKVKTTSRNTFWETLRQNIEDILSSSR
                     KLSQTADERQARAERMRAEREERVAQAEAVARAGSNAKDLYDKVFGPAKQVTDVKEDI
                     VVNEVAGTVTVMGRQREQDLIQEYLDSVQASVQRQVLIEATIAEVDLSNAYQAGVDWS
                     ALATDGSGFNIRQETMGPEVLAGAPRAVIGYVDPNSPFGNLNISVRLLERFGKTRVLS
                     SPKLMALNNQTALLKVVDNRVYFTTEQQTSQAQSSTVQTITTTVNTVSVGVVVSLTPQ
                     VHDNGNVTLLVRPTISRILRFVPDPNPLLTAAPNLIPEITVREMESVLQLTSGQTAIL
                     GGLMQDNTRRDRDQVPYAGNLPRVGDAFAFRDEEVRKSELVIFIRPVVVNNPSLDSDE
                     LKHLRKLLPQIDQTGENP"
     gene            complement(4282..>4990)
                     /locus_tag="VD791_02240"
     CDS             complement(4282..>4990)
                     /locus_tag="VD791_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06820.1"
                     /translation="AWVGAAALLALVAVGAGWLQRGMQPELPAMPSAAAPTTAAPAPT
                     PGATQAAVAPQPGAPDAPLDTALAVQATAPAAVAPAPTAAATAAGTADAMIAQPATGE
                     TTAFATARALPAAPSGADAAGPDLLEQRLLATERWLAEADAGVFSIQLLGSTDQQMLK
                     RYLNDLTNYIERDRIFVYRTVANQAPSMTVLYGEFAARADAVRSLDGLPPELRANRPY
                     IRTVQGIRSELGLDKPS"
BASE COUNT          677 a         1676 c         1777 g          860 t
ORIGIN      
        1 gcccgtcctg aggggcgcgc gtctccgcga tattcatgcc gctgatgacc ttcaggcgca
       61 ccgcgatgcc aggccagtag ttcttgtgca ggctgcgcac ctgctcgagc acgccgtcga
      121 tccggtaacg cacgcgcagg aacgcgaact cgggttcgaa gtggatatcc gaggcgccgc
      181 gctttaccgc atccaccaac agcgcgccga ccaggcgcac gatgggctgc tgatactcat
      241 cgcctccgcc ctgcaggctc tgataatcga tctcgccggt ctcgatctcg cgcaggatgc
      301 cgtcgaccga cagctcgtga ccgtagaact ggtcgatatg gtcctcgagc tgggcatcgc
      361 cggccagcac cgtcttgatc ttgatctgct gaccgatggt ggcgcgcagc tgatcgagcg
      421 ccaccatgtt gaacacctcg gtgatggcca cggtcagcgc ctgcgcggcg ccgtcgaagg
      481 cgatcggcag cagccggtgg cggcgggcga agtcctgggg gatcaaccgg accgcttcgc
      541 cgtccaccac cacctgcgtg aggtcgatgc tttcctggcc gatggtccgg gccatgatgt
      601 cccggatcac ggcctcggtg acgaagccca ggcgcaccag caggcggccc aacggaacgc
      661 tctggttctt ctgctcggtg agcgcgatgc gcagctgatc gggtgtgatc agcccctgct
      721 gcaccaggag ctcgcccagg cgcagcttct tgcgctgctc cgccatgggc ccagcgctac
      781 tcgctgccaa cgcgcgcgga gagctgccca acgcgttcct gcacgcgagc ctgctcgaag
      841 ctggcgtgcc ccctgagtga gctcaattcc agcgccctgc ggtaatagtt cagggcgatc
      901 ttcgcctggc ccagatgctc caggcccacc gcgaggttgt atgcgtagtc ggggttgtcc
      961 ggctgcagct ggtaagcctg gaagtaggcc ggctgcgcta gcggccagcg cccctggcgc
     1021 gcatacaggt tgccgagcgc gaagtacagg aatccggtcg gaccgcgcgc gatgagctgc
     1081 ttgagccgcg tctccgactg cagcggatcg gcctggccga ggagcgagat cagcccggcc
     1141 tgcgccgtgc tgttgcgcgg ctcgagctcg agcgcgcgcg cgtaatggag cgcggcctcc
     1201 tcgccgttgc ctgcgcgcgt gtcgatggcg gccaagccca gcagcgcgtc cacgttgtgc
     1261 ggctcgatgc gcagcacctc ctgatacagg gcgcgcgcct cctcgaaccg gccctgattc
     1321 aacgcttccc acgcctgcat cagggggacc gacggcgggg tggcgcttgc cggcggcgcg
     1381 cgcaccgcga cgctgtcatc gagcgtggcg gcggcttcca cttccacggt ggcgcggcga
     1441 gggggaggct tctcgcgcgc cgccgatgcg acgggacgct cggcggtgcg gcgggaggcc
     1501 ttggcctgcg gcattccggt gaacgggcgc tcggcggctt ctccggcgcc ggcttcggcc
     1561 ggcggcaccg ccgcggcgcg cgccggtgtg gacagcgtac ccggcggcgt ggacagcgta
     1621 ccggccggcg tggacagggt acccgccggt gtggacggcg ctgccgacgc cgaggacgca
     1681 actgcagcgg cggccggctg agcaggctgc gcttgctcga tgcgacccgc gtgcggcggc
     1741 ggggtgcggc gtttcgcggc gctgggcggc ttggactgca ggaaggaggt caggaaatcc
     1801 ccgcgcagca ccgccggatg gaagatctgc agatagacgt aagcgccgta gaacactccg
     1861 aagatggcgg cggcgatggc gaaggcgtgc accggccggt cgcggaccca gtccagcgca
     1921 ccggtgctgc gcgcctcgtt cgcgcgcagg atggtggcgg cgtgggccga tccgccggcg
     1981 gcggttgccg gccgcagcac cggccgctcc ggtggccgtg cgggcgcgat ggtcggctcg
     2041 ggggcggctg tcagcgccgg ctcgtcttcc ggttccggat cgaacaactc gtcatcatcg
     2101 tccgtgaggg acagcgcgtc cgggtcggcc ggcagctcgc cgagctcggg ctcgcgcggc
     2161 cccggctcgt gcagccccga ttcgggcgcc ccgggttcat gcgacccggg ctcgtacggc
     2221 cccggttcgt acggcccggc ttcgtgcggc tcgcgttcgt acggcaacgc gtcgcgctcc
     2281 ggctcctgct ccgggcctcc gctctcgcga ttcttggccg ccttttgcag cgcttgcagc
     2341 agcaggctca tggattctcg ccggtctggt cgatctgggg caggagcttg cgcaggtgct
     2401 tgagctcgtc gctgtcgagc gacgggttgt tgaccaccac tgggcggatg aagatgacca
     2461 gctcgctctt gcgcacttcc tcgtcgcgga aggcgaaggc atcgcccacg cgcggcaagt
     2521 tccctgcgta gggcacctgg tcgcgatcgc gccgcgtgtt gtcctgcatc aggccgccca
     2581 ggatagcagt ctgcccgctg gtgagctgca gcaccgattc catctcgcgc accgtgattt
     2641 cgggtatcag attgggcgcg gcggtcagca gcgggttggg atccggcacg aagcgcagga
     2701 tgcggctgat ggtcggccgc accagcagcg tgacgttgcc gttgtcgtgc acctgcgggg
     2761 tcaggctcac cacgactccg acggacacgg tgttaaccgt ggtggtgatg gtctgcaccg
     2821 tgctgctctg cgcctggctg gtctgctgct cggtggtgaa gtacacgcgg ttgtccacca
     2881 ccttgagcag cgccgtctgg ttgttgagtg ccatcagctt cggactggac aggacgcgcg
     2941 tcttgccgaa ccgctcgagc aggcgcaccg agatgttcag gtttccgaac ggggagttgg
     3001 gatcgacgta gccgatcacc gcgcgcggcg cgcctgccag gacctcgggg cccatcgtct
     3061 cctggcggat gttgaagccc gagccgtcgg tggcgagcgc gctccagtcc accccggcct
     3121 ggtaggcgtt ggacagatcg acttcggcga tggtggcctc gatcagcacc tggcgctgca
     3181 ccgaggcttg cacgctgtcg aggtattcct ggatcaggtc ctgctcccgc tggcgcccca
     3241 tcacggtgac ggtcccggcg acctcgttca ccacgatgtc ttccttgacg tcggtcacct
     3301 gcttggcggg accgaacacc ttgtcgtaca ggtccttggc gttggaaccg gcgcgcgcga
     3361 ccgcctcggc ctgcgccacc cgctcctcgc gctccgcgcg catgcgctcg gcgcgcgcct
     3421 ggcgttcgtc ggcggtttgc gagagcttgc ggctcgagct gaggatgtcc tcgatgttct
     3481 ggcgcagcgt ttcccagaac gtgttcctgc tggtggtctt gaccttggtc tgcgatgcgc
     3541 ccaccgcctg gttggtcagg tcggaagcgg tgcccacctg cccggtgacg ccgatcgtcg
     3601 agtcggtgtc gcgcaccatg ttcacgtagt tgacccggta ggtcttcagg tacggggtgt
     3661 cgggcgagac cacgatggtc ctgccctcca cgcggtagcg catgttcacc tgccgggaga
     3721 tgcgctccag aatcgagggc agcgtctcgt cgatggcgtt caggctcacc aggccttgca
     3781 ggccgggatg gatgtcgatg ttctccttgg tatcgcgcgc cagcgcgagc agcagctcct
     3841 tcaccggcac ttcgtgcacc accacgctgt aggtcgtggg tttggtcctg ggcctgggcg
     3901 gcgggacgaa gggggagttg gtcagcggcg gcaggatgcg ccgcgtttcc tcgatcggtg
     3961 tcaccgtgtc cgcgctcaag tgcgcatcgg acggcgggac cgtgggccgg taggtggtgc
     4021 aggcgccgga cgcgagcaga gcggccccgg cgagcagcgc gcccggcaag cgcgggatgc
     4081 gcgttgccgc tgcgcgcgac attcgcgggg aacgccacgc aatcatccgc tgcatcgccg
     4141 ccaagtgtga ctccgggttc gtgttgatgc ttcgattgtt atgattgagt ccgcccgctc
     4201 gcgggatcgg ctcgctgcgc ttgccccgcg accggcgcgc tgggcgcggg cgttgcgccg
     4261 ccgcatgccc cggagccgat ctcaggaagg tttgtccagg ccgagctcgc tgcgaattcc
     4321 ctgcaccgtc cgaatgtacg gtcggttcgc gcgcagttcc ggcggcagtc catccaggga
     4381 tcggacggca tccgcacggg ccgcgaactc gccgtacaag acggtcatcg aaggcgcttg
     4441 gttcgccacg gtacggtaca caaaaatcct atctctttca atgtaatttg tcagatcatt
     4501 gagatagcgc ttcagcatct gctgatcggt cgaaccgagc agctggatgc tgaacacgcc
     4561 ggcatcggcc tcggccagcc agcgctcggt ggcaagcaat cgctgttcca gtaagtcggg
     4621 acccgcggca tcggcgccgg acggtgccgc gggcagcgcg cgcgcagtcg cgaacgcggt
     4681 ggtttcgccc gttgccggtt gcgcgatcat cgcgtcggcg gtcccggcag cggtcgcggc
     4741 agcggtcgga gccggcgcga ccgccgcagg agcggtcgcc tgcacggcga gcgcggtgtc
     4801 gagcggcgcg tcgggcgcac cgggctgcgg tgcgaccgcc gcctgcgtcg cgcccggcgt
     4861 cggagcgggc gctgcggtcg tgggcgccgc cgccgagggc attgccggga gctcgggctg
     4921 catgccacgc tgaagccacc ccgcgccaac ggccacgagc gcgagcagcg ccgccgcccc
     4981 cacccacgcc
//
LOCUS       DASPGE010000072         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_842481, whole genome shotgun sequence.
ACCESSION   DASPGE010000072 DASPGE010000000
VERSION     DASPGE010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842481"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..1210)
                     /locus_tag="VD791_02245"
     CDS             complement(251..1210)
                     /locus_tag="VD791_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HXV06821.1"
                     /translation="MLQIGDGVKKWAVLAPTAALPRTLRVAARRHWLARLELAKARRT
                     DLLIIGHPKSGNTWLRVMMSRLYQVRHGLPASVIVTSDELARRNPAIPRISATNGHYS
                     YEGIVGDALAPDAPDNALRHKPIVLLARNPCDIAVSWYFQFTKRQSAHKQELINHFIA
                     HPIDRRTIGMWDFVRHSDIGLPFLIDYLNGWERSLQKLDRALTIRYEDLRAQPAETLG
                     RITRLMGESFTDAEMTEAVTFGSFDNLRQLESKGFFRQGGLTLRNPNDPESFKVRRAK
                     VNGYRDYFAPEQVAELEALVAERLSPTFGYQAAGRDQPQRMAG"
     gene            complement(1382..>2339)
                     /locus_tag="VD791_02250"
     CDS             complement(1382..>2339)
                     /locus_tag="VD791_02250"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXV06822.1"
                     /translation="RHARKRVDRSPMLRPARWTLEAAVMAGLWGLSASLSPERASAAG
                     RRLLQRLGPSLKNTTHLRRNLGLAFPDRSAPELDRLVRQVWGQMGAVLAEYPHLGAIC
                     AGEGGHRVRTVIRFDLERLRRQNRPAIFVTAHLANWEIAAAVGARADLPLTVVYTPIE
                     NPWIDRRLYRYRQALGCKMIAREDALRPLMGELAAGRSVGLLMDQRTDSGAPIPFFGI
                     EKLTTLVPARLALRFDCDLIPVRVERLQGVRFRVTAFEPVSAGAEDRTEQERAMAMTR
                     QVHALFESWIKEAPEAWFCSNRRWPKKAQPPRAALVQAAPAH"
BASE COUNT          370 a          815 c          777 g          377 t
ORIGIN      
        1 gggttcacgc atttggacga acttgcgctc gcacggccag ccgaggacgt cggcgatcac
       61 ctcgacctga gcgttgtcgc cgcgcttgtc gccgaggatc aaccagacgc gcggcgctgc
      121 cgcgctttcg gacgtccgtc gatcggggag atcctgcagc gcgcgcaccg gggacgcctc
      181 tagcgcgccg ccccccgcgc gggccatggc tcgccgcagc gcggttctcg gggcagaaat
      241 actcgcgaca tcagcccgcc atgcgctgcg gctgatcacg gcccgccgcc tgatagccga
      301 aggtggggga caggcgctcg gccaccagcg cctccagctc ggccacctgc tcgggcgcga
      361 agtagtcgcg atagccgttg accttggccc gacgcacctt gaagctctcg ggatcgttgg
      421 ggttgcgcaa cgtcagccca ccctggcgga agaacccttt gctctccagc tggcgcagat
      481 tgtcgaacga gccgaacgtc accgcttccg tcatctcggc gtcggtgaac gactcgccca
      541 tcagcctggt gatccggccg agggtctcgg caggctgcgc ccgcagatcc tcgtaccgga
      601 tcgtgagcgc acgatccagc ttttgcaggc tgcgctccca accattgaga tagtcgatca
      661 gaaacggcag gccgatatcg ctgtgacgca cgaagtccca catgccgatc gtgcgccggt
      721 cgatcgggtg cgcgatgaag tggttgatca actcctgctt gtgggccgac tgccgcttgg
      781 tgaactggaa gtaccaggac accgcgatat cgcaggggtt gcgagccagc agaacgatcg
      841 gcttgtggcg cagcgcgttg tcgggggcgt ccggcgcgag cgcgtcgccg acgatcccct
      901 cgtagctgta gtggccgttg gtcgcgctga tgcgcgggat cgcggggttg cgccgggcga
      961 gctcgtccga ggtgacgatc accgatgccg gcaggccatg gcgcacctgg tagaggcgcg
     1021 acatcatcac gcgcagccag gtattgccgc tcttggggtg gccaatgatc aacagatcgg
     1081 tgcgcctcgc cttggcgagc tccagccggg ccaaccaatg cctccgggca gccacgcgca
     1141 gggttcgagg cagggccgcg gtgggcgcga gcaccgccca tttcttgacg ccgtcgccga
     1201 tctgcaacat cagttctcgc ctgaaagtgg agcgcgctca tgccgatccg gcagacccgc
     1261 cgctacgcgc ccggcgctgc ctagcagatc cttcaaccgc ctgcaacgac ggtcccggtc
     1321 gatcggtcaa aggcccagtg ccacaaagcg ccacgcggca cttgggcctt ctgttcgcga
     1381 tctagtgcgc cggcgccgcc tgcacaagcg cggcacgggg cggctgcgcc ttctttggcc
     1441 atcggcggtt ggagcaaaac cacgcctccg gcgcctcttt gatccagctc tcgaacagtg
     1501 cgtggacctg ccgggtcatg gccatggcgc gctcctgctc ggtgcggtcc tcggcgcccg
     1561 cgctcaccgg ctcgaacgcg gtcacccgga agcgcacgcc ctgcaggcgc tcgacccgca
     1621 ccgggatcag gtcgcagtcg aaccgcaacg ccaggcgtgc cggtaccagg gtggtcagct
     1681 tttcgatgcc gaagaagggg attggtgcgc cgctgtcggt gcgctggtcc atcagcaggc
     1741 cgaccgagcg cccggcagcc agttctccca tcaacggccg gagagcatcc tcccgcgcga
     1801 tcatcttgca gcccagcgcc tgtcggtagc gatagaggcg gcggtcaatc caagggttct
     1861 caatcggcgt gtagaccacg gtcaacggca agtctgcgcg cgcgcctacc gccgcggcaa
     1921 tttcccagtt ggcaagatgc gccgtcacga agatcgccgg ccgattctgc cgtcgcaggc
     1981 gctcgagatc aaagcggatc acggtcctca cccgatgccc cccctcgccg gcgcagatcg
     2041 cgccgaggtg cggatactcg gccaggacgg cgcccatctg cccccagacc tggcgcacca
     2101 gtcgatcaag ctcgggggcg ctgcggtcgg ggaaggcgag gccaaggttg cggcgcaggt
     2161 gcgtagtgtt cttcaggctt ggccccagcc gctgcagcag tcgccggccg gccgccgacg
     2221 cccgttccgg cgacagactt gcgctcagcc cccacagccc tgccatgacc gctgcctcca
     2281 aggtccagcg tgcaggccgc agcatgggcg atcggtcgac gcgcttgcgc gcgtgcctg
//
LOCUS       DASPGE010000073         5363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_257689, whole genome shotgun sequence.
ACCESSION   DASPGE010000073 DASPGE010000000
VERSION     DASPGE010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257689"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VD791_02255"
     CDS             <1..1047
                     /locus_tag="VD791_02255"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HXV06823.1"
                     /translation="RRLGMTGVSEAIRAAGSLAELVRAAGDVRTLAESLVAQGVAAEQ
                     LTRIVSTLNDRVTARLIELECAAAGIGAGELCWMAFGSEGRHEQTLATDQDNGILFPD
                     PPDGDGATRRARLLPLARRINEGLAACGFPLCKGEVMAGNPRWCLSLSEWKSTFAEWM
                     DKADGQALLNATIFFDFRGLWGRSDLADTLRAWLAHKVSGRSLFLRMMAENALRNAPP
                     LGVLRDFAVERHADAQDSLDLKLNGVAPFVDAARILSLQAGDQHTGTVERLRALVRAH
                     RLDAAEVEAWVDAFHFIQMLRLRHQQQQLASGHVPDNFVQPDRLNDLERRILKEAFRQ
                     SRKLQERLRLDFRL"
     gene            1102..1803
                     /locus_tag="VD791_02260"
     CDS             1102..1803
                     /locus_tag="VD791_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HXV06824.1"
                     /translation="MRLFAPWLGRARESDVAVAARSEWSRLTYPSPGASLDLLRFVCV
                     DTETSGLDPGRDRLISIGACAIDAGGIRIDDTFSALLRQEQPSTADNILIHGIGHGAQ
                     AAGDVPEQALAGYLRFAGRDALAGYHAAFDLSVLQRAVRGALGIAYRPPCLDLALLLP
                     ALEAQGGAWELDRWLGLYGLRAFARHHALADAAATGELFLIALQRARARGLRTLGDLV
                     RLQKEQSHLTRMARN"
     gene            1976..2236
                     /locus_tag="VD791_02265"
     CDS             1976..2236
                     /locus_tag="VD791_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HXV06825.1"
                     /translation="MQLTEKHQEYWRRNLVITGILLVIWFVVTFVVQYFAVPLEEVTF
                     FGFPLAFYMGAQGSLIIYVLVIWYYARRMGKLDQEYDVAEGD"
     gene            2240..4297
                     /locus_tag="VD791_02270"
     CDS             2240..4297
                     /locus_tag="VD791_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HXV06826.1"
                     /translation="MATASQTAFTQQLRKYYGFYTGGFAIFVVLLAIAEQLGLPRVWI
                     GYVFLFATIGLYAGIGIISRTADISEYYVAGRRVPALFNGMATGADWMSAASFIGMAG
                     TLYAMGYDGLAFIMGWTGGYVLVALLLAPYLRKFGQFTIPDFLGARYDGNLPRMIGVI
                     AAILASLVYVVAQIYGVGLIVSRFTGFAFGIGVFLGLGGILVCSFLGGMRAVTWTQVA
                     QYIILIVAYLIPVVMLSIKFTDNPIPQLAYGSVLQKVTEREKVLRADPAQQEVREIFK
                     QRAAEIDDKLAALEQSHEADRAALESKLAELRSAGAPQGEIDVAQKALDEFPKDVGAY
                     KEQLSARKASLLARAQPITPHATPFPGKDEAERNAARKNFLALIFCLMVGTAALPHIL
                     MRYYTTPSVSEARKSVFWSLFFIFLLYFTAPALAVLVKYDVYSNLVGTPIADLPAWLS
                     AWSHVDKTLASVTDVNGDGILQLAELTLGQDIIVLATPEIAGLPYVISGLVAAGGLAA
                     ALSTADGLLLTVANALSHDVYYKMLDPNASTKRRVAISKALLLVVALLAAYWASLKPG
                     NILFLVSAAFSLAASAFFPALVLGIFWKRATKWGAVAGMVAGLGVCVWYIYTRYAFFG
                     INAPLIWDIKPISAATFGVPVGFVVIAVVSMLTPAPKRQLQELVEHVRYPHLRGDIDT
                     QAT"
     gene            4349..4648
                     /locus_tag="VD791_02275"
     CDS             4349..4648
                     /locus_tag="VD791_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06827.1"
                     /translation="MPVEITLVSVARALVEVAGWFLLGQGVLYVLAGARRDQNPVYRL
                     FRLITSPVVRATRALTPRVIVDRHVPVVAFFLLFWIWIALQVLKRHLCALHGLAC"
     gene            complement(4701..>5363)
                     /locus_tag="VD791_02280"
     CDS             complement(4701..>5363)
                     /locus_tag="VD791_02280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV06828.1"
                     /translation="VAISYVGHREGASATVQAVQAAGRRGLAVQLDQRGPAAIDASVG
                     AVVQHFGRVDILVNNAAWNIGIPFPSLDALTADIWDRVLETNLRGPYLLSRAFALHLR
                     AHGAGRIVNIASVAGLFPGGSSIAYASSKAGLIHLTRCLAVALAPEVTVNCIAPGLVE
                     GTRMAQRIPEKMARAARGQAVLGRTASAQDIAEQVVTFCRAESVTGQVLVIDGGMPGS
                     MH"
BASE COUNT          791 a         1839 c         1824 g          909 t
ORIGIN      
        1 cggcgcttgg gcatgacggg agtgagcgag gcgatccgcg ccgccggctc gctggccgag
       61 ctggtacgcg cggcgggcga cgtgcgcacg cttgccgaga gcctggtggc gcagggcgtg
      121 gccgcggagc agctcacgcg catcgtctcc accctgaacg atcgggtcac tgcccggctg
      181 atcgagctgg agtgcgctgc cgccggcatc ggcgccggag agctgtgctg gatggcattc
      241 gggtccgagg ggcggcacga gcagacgctg gccaccgacc aggacaacgg cattctgttt
      301 cccgatccgc cggacggcga cggcgcgaca cggcgcgccc ggctgctgcc gctcgccagg
      361 cgcatcaacg aagggctggc ggcctgcggc ttcccactgt gcaagggcga ggtgatggcc
      421 ggcaatccgc gctggtgcct gagcctgtcg gaatggaaga gcactttcgc agagtggatg
      481 gacaaggccg atgggcaggc gttgctgaac gccaccatct tcttcgactt ccgcggcttg
      541 tgggggcgca gcgacctggc cgatacgctg cgcgcctggc tcgcgcacaa ggtttccggt
      601 cgctcgctgt tcctgcgcat gatggcggag aacgcgctgc gcaacgcgcc gccgctgggc
      661 gtgctgcgcg atttcgccgt ggagcggcac gccgatgcgc aggactcgct ggatctgaag
      721 ctcaacgggg tggctccgtt cgtcgacgcg gcgcgcatcc tgagcctgca ggcgggcgac
      781 cagcacacgg gcaccgttga gcgcctgcgc gccctggtgc gtgctcaccg cctggacgcc
      841 gcggaggtgg aggcgtgggt ggacgctttc cacttcatcc agatgctgcg cctgcggcac
      901 cagcagcagc agctcgcgtc cggccatgtg ccggacaact tcgtgcagcc cgaccggctt
      961 aacgatcttg agcgccgcat cctcaaggaa gccttccgcc agtcgcgcaa gctgcaggag
     1021 cggctgcggc tggatttccg gttatgaccg ggcccgccgg cggggcagcg ggatccgcgc
     1081 cgtgaaccgg ggcggttgcg tgtgaggctc ttcgccccgt ggttgggccg ggcacgcgaa
     1141 tccgacgtcg ccgtcgccgc ccgctcggaa tggagccggc tcacctaccc ttcgcccggc
     1201 gcgtccctgg acctactgcg cttcgtctgc gtggacaccg aaaccagcgg gctggaccct
     1261 gggcgcgacc ggctcatctc gatcggcgcc tgtgccatcg acgcgggcgg tattcgcatc
     1321 gacgacacct tctcggccct gttgcgccag gagcaaccca gcaccgcgga caacatcctg
     1381 atccacggca tcggacacgg cgcacaggcc gccggggacg tgccggagca ggcgttggcc
     1441 gggtacttgc ggttcgctgg acgcgacgca ctcgccggct atcacgccgc gttcgacttg
     1501 agcgtgctcc agcgcgcggt gcgcggcgcg ctcgggatcg cctaccggcc gccttgcctg
     1561 gacctggcgc tgctgctgcc cgccctcgag gcgcagggcg gcgcatggga gctcgaccgc
     1621 tggctcgggt tgtacgggct gcgagccttt gcgcggcacc atgcgctggc cgatgcggcg
     1681 gccaccggag agctgttcct gatcgcgctg caaagggccc gggctcgggg tttgcgcacg
     1741 ttgggcgacc tggtgcgctt gcagaaggag cagagccacc tcacccgcat ggcgcggaac
     1801 tgagcccgac cgcggcaaga gctcggaggg catgcggggg ggctgcgcgc cccccgcacg
     1861 gcaccctccc ccgaacacct gcacgccggt atactggcct cgccgcggcc ctcgggcgga
     1921 ccgtcgggcg acaaacgcgc cgccaggccg gcgataacaa catgggagga gagcaatgca
     1981 gctcaccgag aagcaccagg agtactggcg tcgcaacctg gtcatcaccg gcatcctgct
     2041 ggtgatctgg ttcgtggtca ccttcgtggt gcagtacttc gccgtgccgc tggaggaggt
     2101 cactttcttc ggcttcccgc tggccttcta catgggcgcc caaggctcgc tcattatcta
     2161 cgtgctcgtc atctggtact acgcacgccg catgggcaag ctcgaccagg agtacgacgt
     2221 cgcggagggg gactgatcga tggccaccgc atcccagacc gctttcacgc agcagctgag
     2281 gaagtactac ggcttctaca ccggcggctt cgccatcttc gtcgtgctgc tggcgatcgc
     2341 cgagcagctc ggcctgccgc gcgtgtggat cgggtacgtg ttcctgttcg ccaccatagg
     2401 cctgtacgcc gggatcggca tcatcagccg caccgccgac atctccgagt actacgtcgc
     2461 cggccgccgg gtgccggcgc tgttcaacgg catggcgacc ggagccgact ggatgagcgc
     2521 cgcctcgttc atcggcatgg cgggaacgct ctacgccatg ggctacgacg ggctcgcctt
     2581 catcatgggc tggaccgggg gttacgtgtt ggtggccctg ctgctggcgc cttacttgcg
     2641 caagttcggg cagttcacca tcccagactt cctgggcgca cgctacgacg gcaacctgcc
     2701 gcgcatgatc ggcgtgatcg ccgccatcct cgcctccctg gtatacgtgg tggcgcagat
     2761 ctacggcgtt ggcctcatcg tcagccgctt caccggcttc gcgttcggca tcggcgtgtt
     2821 cctgggcctg ggcggcatcc tggtgtgctc cttcctgggc gggatgcggg cggtgacctg
     2881 gacgcaggtg gcccagtaca tcatccttat cgtcgcctac ctcatcccgg tggtgatgct
     2941 gtcgatcaag tttaccgaca acccgatccc gcagctcgcc tacggcagcg tgctgcagaa
     3001 ggtgaccgag cgcgagaaag tgctgcgcgc cgatccggcg cagcaagagg tgcgcgagat
     3061 cttcaagcaa cgcgcggccg agatcgacga caagctggct gccctcgagc aatcgcacga
     3121 ggccgaccgc gccgcgctcg agtccaagct cgccgagctg cgcagtgccg gcgcgccgca
     3181 gggcgagatc gacgtcgccc agaaggcact cgacgagttt ccgaaggacg tgggcgccta
     3241 caaggagcag ctcagcgccc gcaaagcgtc actccttgcg cgcgcccagc cgataacgcc
     3301 gcacgctacc cctttccccg gcaaggacga ggcggaacgc aacgcggcgc gcaagaactt
     3361 cctggcgctg atcttctgtc tgatggtcgg cactgcggcg ctgccgcaca tcctgatgcg
     3421 ctactacacc accccgagcg tcagcgaggc gcgcaagtcg gtgttctggt cgctgttctt
     3481 catcttcctg ctgtatttca cggcgccggc tctggccgta ttggtgaagt acgacgtcta
     3541 cagcaacctg gtggggacgc cgatcgcgga cctccctgcg tggctctcgg cctggtcgca
     3601 cgtcgacaag acgctggcat cggtgaccga cgtgaacggc gacggcatcc tgcaactcgc
     3661 agagctgacg ctggggcagg acatcatcgt gctcgccaca ccggagatcg ccgggctgcc
     3721 ctacgtgatc tcgggactgg tggccgccgg cgggctggcc gcggcgctct cgaccgccga
     3781 cggcttgctg ctcacggtgg ccaacgcgct gtcgcacgac gtgtactaca agatgctcga
     3841 cccgaatgct tccaccaaac gacgggtggc gatctcgaag gcgctgctcc tggtggtggc
     3901 cttgctggcc gcctactggg cgtcgctcaa accgggcaac atcctgttcc tggtgagcgc
     3961 cgccttctcg ctcgcggcct cggcgttctt cccggcactc gtcctcggca tcttctggaa
     4021 gcgcgcgacc aagtgggggg cggtcgccgg catggtggcg ggacttggcg tgtgcgtgtg
     4081 gtacatctac acgcgctacg cgttcttcgg tatcaacgcg ccgctcatct gggacatcaa
     4141 gccgatctcc gcggcgacct tcggcgtgcc ggtgggcttt gtcgtgatcg ccgtggtgag
     4201 catgctcacc cccgctccga agaggcagct gcaggaactg gtggagcacg tgcgctatcc
     4261 gcatctgcgc ggggacatcg acacgcaggc cacctgagag cctggtcggc ttgccgaggc
     4321 ggccgcgagc cgcctcggct tttcaccgat gcccgttgag atcacgctgg tcagcgtcgc
     4381 gcgcgccctg gtcgaggtcg cgggctggtt cctcctcggg cagggagtgc tgtacgtgct
     4441 cgcgggtgcg cgccgggatc agaaccccgt ctatcgcttg tttcgtctga tcaccagccc
     4501 cgtggtgcgc gcgacgcgtg cccttacgcc aagagtcatc gtggaccggc acgtgccggt
     4561 cgtcgcgttc ttcctcctgt tctggatctg gatcgccttg caggtgctca agcgtcatct
     4621 gtgcgcgctg cacggcctgg cttgctgacc ccggcgaccg gggtggagat gagagaccgg
     4681 ctgcgcaggc gccacccgcg tcagtgcatg ctgcccggca taccaccgtc gatcaccaga
     4741 acctgcccgg tcaccgactc ggcacggcag aaggtgacca cctgctcggc gatgtcctgc
     4801 gcgctcgccg ttctgccgag cactgcctgg ccgcgcgccg cccgcgccat cttctcggga
     4861 atgcgctgcg ccatgcgcgt gccctccacc agccccggcg cgatgcaatt gaccgtcacc
     4921 tccggggcca gcgccaccgc caggcaacga gtgaggtgga tcaaccccgc cttgctggac
     4981 gcgtaggcga tgctgctgcc gcccgggaac agcccggcca ccgaggcgat gttcacgatc
     5041 cggcctgcgc catgcgcgcg cagatgcagc gcgaacgccc tggacagcag gtacggcccg
     5101 cgcagattgg tttcaaggac acggtcccag atatcggccg tcaatgcgtc gagggaaggg
     5161 aacgggatgc cgatgttcca ggcggcgttg ttgaccagga tgtccacccg accgaagtgc
     5221 tggaccacgg ccccgaccga ggcgtcgatg gcggccgggc cgcgctgatc gagttgcacc
     5281 gccagcccgc gccggccggc tgcttgcacc gcctgcacgg tcgcggatgc gccttcgcgg
     5341 tgccccacgt aggagatggc gac
//
LOCUS       DASPGE010000074         9878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1545601, whole genome shotgun sequence.
ACCESSION   DASPGE010000074 DASPGE010000000
VERSION     DASPGE010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9878
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545601"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VD791_02285"
     CDS             <1..711
                     /locus_tag="VD791_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HXV06829.1"
                     /translation="RLRRLAELAEQVDALQDLLYAEHKHKLLIVLQGMDTSGKDGTIR
                     HVFTEVDPLGVRAVSFRAPAGEELEHVYLWRIHRQTPRKGEIVIFNRSHYEDVLVPRV
                     HALIDLAECKRRYRQINDFERMLVENGTILLKFFLHISKAEQRARLQERVDEPNKRWK
                     FNPRDLKERARWDDYMAAYEDAIEATSSEWAPWHVVPSDSKSTRNLIVSSILVDTLEG
                     LKMAYPQPAEDYSEIVVA"
     gene            complement(720..1973)
                     /locus_tag="VD791_02290"
     CDS             complement(720..1973)
                     /locus_tag="VD791_02290"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HXV06830.1"
                     /translation="MNILVLGAGVVGVTSAWYLAADGHAVTVIDRQPGAGLETSFANG
                     GQISVSQAEPWATPEAPAKIVRWLGDESAPLLFRPLLSPRQWRWGLQFLYECLPGRTR
                     DNTVQILHLAIYSAAQLKQLRDQSGVRYDYLERGILQLYFDAGEFEIACARAELIRRG
                     GVDLLVRSPQQCVEIEPALAYSSVAPAGGTYAPSDESGDAHQFTRSLAEICARRGVKF
                     CYDTTVERLEADGERVRAVHVTRGGASEAMQADVVLVCLGVRSPFLLEPLGIRLPVYP
                     VKGYSITLPVARADRAPSVCITDETHKMGVTRLGDRLRVAGTAELDGYDTSINEVRCQ
                     ALLQRVGQLFPEAGDAARVQKWAGLRPATPGNVPVIGRTRLANLFLNTGHGTLGWTLC
                     AGSGRSIADIIGGRTPPVDFRFRRG"
     gene            complement(2007..3020)
                     /locus_tag="VD791_02295"
     CDS             complement(2007..3020)
                     /locus_tag="VD791_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXV06831.1"
                     /translation="MKTSRRNFLKTASAAAAGAPLLAARHAPARAAAREGDPDLPRGL
                     TLATIRRHNQWRLAVRTRRGLLDVAAAREDMDIDAPTSIDELFARGGGNALTRLAEAA
                     VASERAGRWFLREDEAEFGPCVTNPEKILCVGLNYWKHAQEMGAQPPGTPMLFNKYNN
                     ALMGHRGVLRLPTKVARAFDYEVELVVVIGRSARDIGAQQALSYVAGYCTGNDMSARD
                     LQRKTSQFMLGKTCDGFAPIGPWLVGADQVPDPNALDLWCEVNGERRQSSNTRDMVFS
                     CAELVSYASQHMTLKPGDIIFTGTPEGVIAGRKPEQQAWLKPGDRIRCGIEPLGALEF
                     TLA"
     gene            complement(3043..3474)
                     /locus_tag="VD791_02300"
     CDS             complement(3043..3474)
                     /locus_tag="VD791_02300"
                     /inference="COORDINATES: protein motif:HMM:NF042415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY0301 family protein"
                     /protein_id="HXV06832.1"
                     /translation="MRNRLVALALPGLMAGAVGDAAGAEVSCPDRLTVEQRAQAPDGW
                     VVSYSGIGPRLSGVSIFDGQPANRVSLKHTRRRQARRELILTWDLSHSPRSHYLQCSY
                     ERTTASISMPLPPGTTQCEVAYDLTSSYPGGAMPVKRMVCR"
     gene            complement(3481..4266)
                     /locus_tag="VD791_02305"
     CDS             complement(3481..4266)
                     /locus_tag="VD791_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV06833.1"
                     /translation="MDALFSVQDQVVLVSGGSRGIGRALAQGFAERGAQVIIGGREAS
                     VLEATARDISSGAKRVQTAVCDVAKPDDIARTVQAAIAEHGRIDTLLNVAGVNKRMRA
                     ESYSVEDFDGILDINLRGAFLMAQACGRHMIARKCGCIVGIDSFNTYAPLRGVLPYAI
                     SKSGMSMMTRGMATEWGRHGVRVNAIAPGFFPTDLSRKLWAQEKMARWGQAMTPLGKL
                     GELKDLVGAAIFLASPAAAFITGQVLRVDGGTSAGIDWPIDLE"
     gene            complement(4279..4860)
                     /locus_tag="VD791_02310"
     CDS             complement(4279..4860)
                     /locus_tag="VD791_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HXV06834.1"
                     /translation="MFTLRRFLLVCVLLLAVAAHGPQASGVEPVLLVASTELEDPSFA
                     HSVVLVLFPAGGWPTGVILNRPTRLSWGEAFPDEPALRARADPIFFGGPVRINALWFL
                     FRQGSPQGNVLPVLDDLYLSSDGELLDRLLAGKAPVDRFFVGYSGWAPEQLDVEIAQG
                     AWHVLPAELDVILKMDPETMWRELLLRATAVKI"
     gene            complement(4935..6380)
                     /locus_tag="VD791_02315"
     CDS             complement(4935..6380)
                     /locus_tag="VD791_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXV06835.1"
                     /translation="MDAPLSTAQPLPRPLPALRFDAHGKRRVDLRAVIALAAPLFLNS
                     GVQAVLNLTDTWFLGRISTEATAGVGAVYWFVIVFILIFGGVAMAVQTLVAQAHGAGE
                     RTQAARIAWAGLWASLCLIPLFVAASLSGRALLAPFDLPPGIETAALAFWFPRMLGAA
                     AGVALWSATSFFNGIGRTAVTLMVMLIVAAANVPLNQLLIFELGLGVAGAAWATGAAQ
                     LLGVGVALALFLSAPVRREFDCTRHWRPRAEALRRLFSLGVPMGLLPAADLTGLALFQ
                     AMLAALGAVPGAATQIVMMMTSIAYMPAVGIASAGTTLVGQSIGAGDRRWAHKVGNAA
                     ILVNVAYMGLVSLVLALAGPWLLPLFVSPADPNARAVIDIGRVLLWIAAAYQIFDGFN
                     LGAGFCLRGAGDVRVPALLVLLLSWVGFVPLTHMLAFAPGEGWVQALPQFGLGAAGGW
                     SALLLYTGAVGAVMFLRWRSGAWRRIDLSRA"
     gene            6531..7379
                     /locus_tag="VD791_02320"
     CDS             6531..7379
                     /locus_tag="VD791_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HXV06836.1"
                     /translation="MRWGGGRRSANVEDRRGMRVSPGIAGGGLGAIVIVILALLFGVD
                     PRMLTQMLPQESPRGSSGPAQGPAVSGEQDPLAEFVSVVLADTEDTWNELFRRGGAQY
                     VEPTLVLFSSAVQSACGFAQAAVGPFYCPADQKVYIDLSFYQDLRNRFEAPGDFAQAY
                     VIAHEIGHHVQNLLGIAGKVRERQQQVSEAQANALSVRMELQADCLAGIWAHHADRSR
                     QILEQGDVEEALNAAAQIGDDRMQMRGRGYVAPESFTHGSSDQRAHWFRAGLETGELS
                     RCDTFG"
     gene            complement(7447..8049)
                     /locus_tag="VD791_02325"
     CDS             complement(7447..8049)
                     /locus_tag="VD791_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HXV06837.1"
                     /translation="MDAILFDLGGVLIDWNPRYLYQRHFGADVAAMEDFLARVCAPEW
                     NLEMDAGKPSAEAVAERQRAFPEHAALIALWKDEWAQMLRDAIPETVQILAELRARGL
                     RLFALTNWSAETFPVAQARFAFLRWFEDIVVSGEVKLVKPDPRIYRLALERNRLRADR
                     TLFVDDALHNVQAARALGLPAIHFQSAQALRAELALRGLL"
     gene            complement(8206..8472)
                     /locus_tag="VD791_02330"
     CDS             complement(8206..8472)
                     /locus_tag="VD791_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06838.1"
                     /translation="MSADIDLKACTILERLSAELSDATVDYCCTQDLHEFQIVRAGMK
                     HEVGFMERVLELRDIPDIEHVVNRLVEEIKASTQPRRIRIGNNG"
     gene            8684..9124
                     /locus_tag="VD791_02335"
     CDS             8684..9124
                     /locus_tag="VD791_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06839.1"
                     /translation="MGIKLMPFAIALAAVLASGARAEDWDHDANIGAAVQALVAVHRA
                     GGMDAAATFVGDCYATIDGYQDSDVQLQRLEYCAGMDFAGYLVGRQAQVRNGEESPPF
                     FAAGAMFERMQRLDRWVQDPGMNQEVLRAWATTAANSLLAQQQN"
     gene            complement(9173..9565)
                     /locus_tag="VD791_02340"
     CDS             complement(9173..9565)
                     /locus_tag="VD791_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HXV06840.1"
                     /translation="MALTQQQSNQLARALDTRRAVLLEEIRTELERSGEQHYIDLADR
                     VADSGEASVADLLVDLEAAMTDRDMRELREIDAAQQRMRDGSYGTCRDCGGEIDFERL
                     RANPVAVRCIVCQTQHEKSFAHGRTPTL"
BASE COUNT         1540 a         3475 c         3414 g         1449 t
ORIGIN      
        1 cgcctgcgcc ggctcgcgga gctggccgag caggtcgatg cgctgcagga tctgctctac
       61 gccgagcaca agcacaagct gctgatcgtc ctgcagggca tggacacctc gggcaaggac
      121 ggcactatcc gccacgtgtt cacagaagtg gaccccctcg gggtgcgcgc ggtgagcttc
      181 cgcgcgccgg ccggcgagga gctggagcac gtctacctgt ggcgcatcca ccgccagacg
      241 ccgcgcaagg gcgagatcgt gatcttcaac cgcagccatt acgaggacgt gctggtgccg
      301 cgcgtgcacg ccctgatcga cctcgcggag tgcaagcgcc gctaccggca gatcaacgat
      361 ttcgagcgca tgctggtgga gaacggcacc atcctgctga agttcttcct gcacatctcg
      421 aaggcggagc agcgcgcgcg cctgcaggag cgcgtcgacg agccgaacaa gcgctggaag
      481 ttcaacccgc gcgatctcaa ggagcgcgca cgctgggacg actacatggc cgcctacgag
      541 gacgctatcg aggcgacctc cagcgagtgg gcgccgtggc atgtcgttcc gtcggattcg
      601 aagtccacgc gcaatctgat cgtctcctcc atcctggtgg acacgctcga ggggctgaag
      661 atggcctatc cgcagccggc ggaggactac tccgagatcg tggtggcgta gcggggccgc
      721 tagccgcggc ggaagcgaaa atccaccggc ggcgtgcgtc caccgatgat gtcggcgatg
      781 gagcgccccg agccggcgca cagcgtccag ccgagcgtgc cgtgcccggt gttcaggaac
      841 aggttcgcca ggcgcgtgcg gccgatcacc gggacatttc cgggcgtcgc cggccgcagg
      901 cccgcccact tctgtacgcg cgccgcatcg ccggcttcgg ggaagagctg gccgacccgt
      961 tgcagcaacg cctggcagcg cacctcgttg atcgaggtgt cgtaaccgtc cagctcggcc
     1021 gttccggcca cgcgcagtcg atcgcccagg cgcgtcacgc ccatcttgtg cgtctcgtcg
     1081 gtgatgcaca cactcggtgc gcggtccgcg cgcgccaccg gcagcgtgat cgagtagcct
     1141 ttcaccggat agaccggcag tcggatcccc agcggttcga gcaggaacgg cgatcgaacc
     1201 cccaggcaga cgagcacgac gtcggcctgc atggcttcgc ttgcgcctcc gcgcgtcacg
     1261 tgtaccgcgc gcacgcgctc gccgtccgcc tcgaggcgct ccacggtggt gtcgtagcag
     1321 aacttcaccc cgcgccgggc gcagatctcc gccaggctgc gggtgaactg gtgcgcgtcc
     1381 ccgctttcgt cgctcggggc gtacgttccg ccggcgggag ccacgctgct gtacgccagc
     1441 gcgggttcga tctcgacaca ctgctgcggg ctcctcacca gcagatccac gccgccgcgg
     1501 cggatcagct cggcacgcgc gcaggcgatc tcgaactcgc cggcgtcgaa gtagagctgc
     1561 aggatcccgc gctccaggta gtcgtagcgc acgccggact ggtcgcgcag ctgcttgagt
     1621 tgcgcggcgc tgtagatcgc caggtgcaag atctgcacgg tgttgtcgcg cgtgcgcccg
     1681 ggcaggcact cgtacaggaa ctgcaggccc cagcgccact ggcgcgggct caacagcggg
     1741 cgaaacaaca gcggcgcgga ctcgtcgccc agccagcgca cgatcttggc cggcgcttcc
     1801 ggagtggccc agggctcggc ttggctgacc gagatctggc cgccgttggc gaagctcgtc
     1861 tccagcccgg cgccgggctg gcggtcgatg acggtgaccg cgtgcccgtc cgcggccagg
     1921 taccacgcgc tggtgacgcc gaccacgccg gcgcccaata ccaggatgtt catgctcctc
     1981 tcacccggcg cggcgagaac gcgccttcat gccagcgtga actccagcgc cccgagcggc
     2041 tcgatgccgc agcggatgcg gtcgcccggc ttcaaccacg cctgctgctc gggcttgcgg
     2101 ccggcgatca cgccttcggg cgtgccggtg aagatgatat cgccgggctt gagcgtcatg
     2161 tgctgcgagg cgtagctcac cagctcggcg cagctgaaca ccatgtcgcg cgtgttcgag
     2221 ctctgacggc gctcgccgtt gacctcgcac cacaggtcca gcgcgttcgg atcgggcacc
     2281 tggtcggcgc cgaccagcca cggtccgatc ggcgcgaagc catcgcaggt cttgccgagc
     2341 atgaactggc tggtcttgcg ctgcaggtcg cgcgcgctca tgtcgttgcc ggtgcagtag
     2401 ccggccacgt aggacagcgc ctgctgcgcg ccgatgtcgc gcgcgctgcg gccgatcacg
     2461 accaccagct ccacttcgta gtcgaaggca cgcgccacct tggtcgggag ccgcagaacg
     2521 ccgcgatggc ccatcagcgc gttgttgtac ttgttgaaca gcatcggcgt gcccgggggc
     2581 tgcgcgccca tctcctgcgc gtgcttccag tagttcaggc ccacgcacag gatcttttcc
     2641 gggttggtca cgcacggccc gaactcggcc tcgtcttcgc gcagaaacca tcgtccggcg
     2701 cgctcgctcg ccacggcggc ttcggcgagc cgcgtcagcg cattgccgcc gccgcgcgcg
     2761 aacagctcgt cgatgctggt cggcgcgtcg atatccatgt cctcgcgcgc ggcggcgacg
     2821 tcgagcaggc cgcgccgcgt cctcaccgcc aggcgccact ggttgtggcg gcggatcgtc
     2881 gccaacgtga gtccgcgcgg caggtccggg tcgccctcgc gcgcggcggc tcgagcgggc
     2941 gcgtggcggg cggcgagcag cggagcgccg gcggccgccg cgctggcggt cttcaggaag
     3001 ttgcggcgag aggttttcat ccggttcctc cgggtttgcg tcctagcggc aaaccatgcg
     3061 cttcaccggc atcgcgccgc cgggatagct gctggtcaga tcgtaggcga cctcgcattg
     3121 cgtggtccct ggcggcagcg gcatggagat gctggcggtg gtgcgctcgt agctgcattg
     3181 caggtaatgg ctgcgcggcg aatggctcag gtcccaggtc aggatcagct cgcgccgtgc
     3241 ctgccggcgc cgtgtgtgct tcaggctgac gcgattggcg ggctgcccgt cgaagatgct
     3301 cactcccgac aggcgcgggc cgattcccga gtagctcacg acccagccat ccggcgcctg
     3361 cgcccgctgc tcgacggtga gccggtccgg acaagacacc tccgcgccgg ccgcgtcccc
     3421 cacagcgccc gccatcaggc cggggagcgc gagggcgaca agccggttgc gcaccgcgct
     3481 tcactccagg tcgatcggcc agtcgatccc ggcgctcgtg ccgccgtcca cccgcagcac
     3541 ttgcccggtg atgaaggccg cggcgggcga ggcgaggaag atcgccgcac ccaccagatc
     3601 cttcagctcg ccgagcttgc cgagcggcgt catcgcctgt ccccagcgcg ccatcttctc
     3661 ctgtgcccac agcttccgcg acaggtcggt gggaaagaac ccgggggcga tcgcgttcac
     3721 gcggacgcca tgccgccccc actcggtggc catgccgcgc gtcatcatcg acatgccgct
     3781 cttgctgatg gcgtagggca gcacgccgcg caacggcgcg taggtgttga acgagtcgat
     3841 gcccacgatg cagccgcact tgcgcgcgat catgtgccgc ccgcaggcct gcgccatcag
     3901 gaaggcgccg cgcaggttga tgtccaggat cccgtcgaag tcttcgacgc tgtagctttc
     3961 cgcgcgcatg cgcttgttga cgccggccac gttgagcagg gtgtcgatgc ggccgtgctc
     4021 ggcgatcgcg gcctgcaccg tgcgcgcgat gtcgtcgggc ttggccacgt cgcacaccgc
     4081 ggtctgcacg cgctttgcgc cactggaaat gtcccgggcg gtggcctcca gcacgctcgc
     4141 ctcgcgcccg ccgatgatca cctgcgcgcc gcgctcagcg aacccctgcg ccagggcccg
     4201 gccgatgccg cggctgccgc cggagaccag gacgacttga tcctgcaccg agaacagcgc
     4261 gtccacgtct ctcgccgatc agatcttcac cgcggtggcg cgcagcagca gctcgcgcca
     4321 catggtttcg ggatccatct tgaggatgac atccagctcc gccggcagca cgtgccacgc
     4381 gccttgcgcg atctccacgt cgagctgctc cggcgcccag cccgaatagc cgacgaagaa
     4441 gcggtccacc ggcgctttcc ccgccagcag gcggtcgagc agctcgccgt cggagctgag
     4501 gtacaggtcg tcgagcaccg gcagcacgtt gccctgcggg ctcccctggc ggaacaggaa
     4561 ccacagggcg ttgatgcgca ccggaccgcc gaagaagatc ggatccgcgc gcgcgcgcag
     4621 ggccggctcg tcgggaaacg cctcgcccca cgacaggcgc gtaggccggt tgaggatgac
     4681 cccggtcggc caaccgccgg cggggaacag caccagcacc acgctgtgcg cgaagctcgg
     4741 gtcttccagc tcggtgctgg ccaccagcag caccggctcg acgccgctcg cctgcggccc
     4801 atgcgccgcg acggcgagga gcagcacaca cacgagcagg aagcggcgaa gcgtgaacat
     4861 gcgaatgcgg gcaggaatgg ccgaacagta caccgcgctc cgcgttgcgg caagcgcggc
     4921 gtgacgccgg ccgctcacgc gcgcgacagg tcgatgcggc gccaggcgcc cgagcgccag
     4981 cgcaggaaca tcaccgcacc caccgcgccg gtatagagca gcagcgcgga ccagccgccg
     5041 gcggcgccca ggccgaactg cggcagcgct tgcacccagc cctcgcccgg cgcgaaggcg
     5101 agcatgtggg tgagggggac gaagcccacc caggacagca gcagcacgag cagcgcgggc
     5161 acgcgcacgt cgccggcgcc gcgcaggcag aagccggccc ccagattgaa gccgtcgaag
     5221 atctggtagg ccgcggcgat ccacagcagc acgcggccga tgtcgatcac cgctcgcgcg
     5281 ttggggtcgg cgggcgagac gaacagcggc agcagccacg gcccggcgag cgccagcacc
     5341 aggctcacca ggcccatgta ggcgacgttc accaggatcg cggcgttgcc gaccttgtgc
     5401 gcccagcgcc ggtcgcccgc gccgatcgat tgtccgacca gagtggtgcc ggcgctggcg
     5461 atgcccaccg cgggcatgta ggcgatcgag gtcatcatca tgacgatctg cgtggccgcg
     5521 cccgggaccg cgcccagggc ggcgagcatc gcctggaaca gcgccaggcc ggtcaggtcc
     5581 gccgcgggca gcaggcccat cggcaccccg agcgagaaca ggcgccgcaa tgcctcggcg
     5641 cgtggccgcc agtggcgcgt gcaatcgaat tcccggcgca ccggcgcgct gagaaacagc
     5701 gccagcgcga cgcccacgcc gaggagctgg gccgcgccgg tggcccaggc cgccccggcc
     5761 acccccagcc cgagctcgaa gatgaggagc tggttgaggg gcacgttcgc cgcggccacg
     5821 atcagcatca ccatcagggt caccgcggtg cggccgatgc cgttgaagaa gctggtcgcc
     5881 gaccacaacg ccaccccggc tgccgcaccc agcatccgcg gaaaccagaa cgcaagtgcc
     5941 gcggtttcta ttcccggcgg caggtcgaag ggcgccagca acgcgcgccc cgagagcgac
     6001 gcggcgacga acagcgggat caggcacagg ctcgcccaca gcccggccca ggcgatgcgc
     6061 gccgcctgcg tgcgctcccc ggcgccgtgc gcttgcgcga ccagcgtctg caccgccatc
     6121 gccacgccgc cgaagatcag gatgaacacg atcacgaacc agtacaccgc gccgacacct
     6181 gcggtggcct cggtggagat acggccgagg aaccaggtgt cggtgagatt gagcaccgcc
     6241 tgcacgccgc tgttcaggaa cagcggtgcc gcgagcgcga tgacggcgcg caagtccacc
     6301 cggcgcttgc cgtgcgcatc gaagcgcaac gcgggcagcg gccgcggcag gggctgggcg
     6361 gtggagagcg gggcgtccat cggcggatga tgctgttcca gcctgggcgc cggaaaagcg
     6421 aaacgcgcag tgtagcggca gcgcaggctt cacgcccgct tggcggcgtc gcggcgggtt
     6481 tacggctaca ctgtccgcgc taccaatcca tcaccgggac ggcgagcgag atgcgctggg
     6541 gcggaggcag acgcagtgcg aacgtggaag accgccgcgg catgcgcgtg tcgccgggca
     6601 tcgccggtgg cgggctgggc gccatcgtga tcgtgatcct ggcgctgctg ttcggcgtcg
     6661 atccgcgcat gctcacgcag atgctgccgc aggagagccc ccggggaagc tcggggccgg
     6721 cgcagggacc ggccgtcagc ggcgagcagg acccgctggc cgagttcgtg tcagtggtgc
     6781 tggcggacac ggaggacacc tggaacgagc tgttccgccg cggcggggcg caatacgtcg
     6841 agcccacgct ggtgctgttc tccagcgcgg tgcagtcggc ctgcggtttc gcccaggccg
     6901 cggtcggccc cttctattgc ccggccgatc agaaggtgta catcgacctg agcttctatc
     6961 aggacctgcg caatcggttc gaggcgcccg gcgacttcgc gcaggcttac gtcatcgcgc
     7021 acgagatcgg gcatcacgtg cagaacctgc tcggcatcgc cggcaaggtg cgcgaacgcc
     7081 agcagcaggt gtcggaggcg caagccaacg cgctgtcggt gcgcatggag ctgcaggcag
     7141 actgcctggc gggcatctgg gcgcaccacg ccgatcgctc gcggcagatc ctggagcagg
     7201 gcgacgtgga ggaggccctc aacgccgccg cgcagatcgg cgacgatcgc atgcagatgc
     7261 gcggccgcgg ctatgtcgcc ccggagtcct tcacgcacgg cagcagcgac cagcgcgcgc
     7321 actggttccg cgccggcctg gagaccggcg agctgagccg ctgcgacacc ttcggctagc
     7381 tccgggaacc tgagcctggc ccggatggag cgaagcggaa tccgggaagg agcctacgac
     7441 tgcggttcac agcagcccgc gcagcgccag ctcggcgcgc agcgcctgcg cgctctggaa
     7501 gtggatcgcc ggcagaccga gcgcacgcgc cgcctgcacg ttgtgcagcg catcgtccac
     7561 gaagagcgtg cggtcggcgc gcaaccgatt gcgctccagc gcgagccggt agatgcgcgg
     7621 gtcgggcttg accagcttga cctcgcccga caccacgatg tcctcgaacc accgcaggaa
     7681 cgcaaaacgc gcctgcgcca ccggaaaggt ctccgccgac cagttggtga gggcgaacag
     7741 ccgcagcccg cgcgctcgca actcggccag gatctgcacc gtctccggga tcgcatcgcg
     7801 cagcatctgc gcccattcgt ccttccacag cgcgatcagc gcggcgtgct cggggaacgc
     7861 gcgctggcgt tcggctaccg cctcggcgga cggcttcccc gcgtccatct cgaggttcca
     7921 ttcgggcgcg cacacgcgcg cgaggaagtc ctccatcgcc gcgacgtctg cgccgaagtg
     7981 gcgctggtac agatagcgcg ggttccagtc gatcaggacc ccgccgaggt cgaagagtat
     8041 cgcgtccatg ccggttctgc ggtcgtgggc ccgcagccta gcaggatcgg gcgcggcaag
     8101 cagcccgcgc agtgcaatcg aacggaggtt gggtttgtgc gcccggagga cggcgcgggt
     8161 gccccccaag cgctcgcgcg cgcggccggg cgaggcggct gcggatcagc cgttgttgcc
     8221 gatgcggatg cgccgcggct gggtgctggc cttgatctct tcgaccagac gattgacgac
     8281 gtgctcgatg tcggggatgt cgcgcagctc caggacgcgc tccatgaacc cgacctcgtg
     8341 cttcatgccc gcacgcacga tctggaactc gtgcagatcc tgcgtgcagc agtaatccac
     8401 ggtggcgtcg gacagctccg cgctcaggcg ttccaggatg gtgcaggctt tcaggtcgat
     8461 gtctgcgctc attccgtttc ccttcacaag ccggtggcaa aacgccggct cacccctctt
     8521 acagcagttt ccgttccatc gcggtgcagt aattgacagt tgacagttca cgaagagttc
     8581 tcgatcgtgc ccgccggcgg cggccgcggg ccggcaggac cgcaccttgc tcggggtctt
     8641 gtcaagcgcg gatgccgggg catcccgcga cactggaggt catatgggaa tcaagctcat
     8701 gccgttcgcg attgcgctgg cggccgtcct ggcgagcggc gcccgggccg aggactggga
     8761 ccacgacgcc aacatcggcg ccgcggtgca agcgctggtc gccgtgcatc gcgcgggcgg
     8821 catggatgcc gccgccacct tcgtcggaga ctgctacgcg acgatcgacg gttaccagga
     8881 ctcggacgtg cagctccagc gcctggaata ctgcgcgggg atggacttcg ccggctacct
     8941 ggtgggccgt caggcccagg tgcgcaacgg cgaggagagc ccgccgttct tcgcggccgg
     9001 cgcgatgttc gagcgcatgc agcggctcga ccgatgggtg caggacccgg ggatgaatca
     9061 agaagtcctg cgcgcatggg ccaccaccgc cgcgaactcc ctgctcgcgc agcagcagaa
     9121 ctgaagcggc tgcgccccgc cgaccgcggg cgctagattg tcgcgtgcgc gctcacagcg
     9181 tcggcgtgcg gccgtgagcg aagctcttct cgtgctgcgt ctggcacacg atgcagcgca
     9241 ccgccacagg gttggcgcgc aaccgctcga agtcgatctc gccgccgcaa tctcggcacg
     9301 tgccatagct gccgtcgcgc atgcgctgct gcgccgcgtc gatctcgcgc aactcgcgca
     9361 tgtcccggtc ggtcatcgcc gcctcgagat ccaccagcag gtcggccacc gacgcctcac
     9421 cgctgtcggc cacgcggtcg gcgaggtcga tgtagtgctg ctcgcccgag cgctccagtt
     9481 ccgtgcggat ctcctcgagc agcacggcgc gccgcgtgtc gagggcccgg gcgagctggt
     9541 tcgattgttg ttgcgtcagt gccatgttgg ctccttgcgg ggtatcggtt cggggactgc
     9601 ggattgtcgg gctgcgccta ttatgaccgg cccggcgcca gcgaagatcc ggttcgccgg
     9661 caacgctcca cgccgtttgc tccccaggct gggggcgagg ccttcctcca gccgcttctc
     9721 atcgggccag gccggggctt tcgcaattcg cgattcgcga atagactgtg cgggaacccc
     9781 cggatgccgg gtccctctat gagcctgaaa ccccaggacg tctgtgtcct gctcaagatc
     9841 gtagccctgg aacgcacgcc ctggtcctac agccaact
//
LOCUS       DASPGE010000075         6297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_961195, whole genome shotgun sequence.
ACCESSION   DASPGE010000075 DASPGE010000000
VERSION     DASPGE010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961195"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1977)
                     /locus_tag="VD791_02345"
     CDS             complement(<1..1977)
                     /locus_tag="VD791_02345"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:NF002837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HXV06841.1"
                     /translation="MQSSVPAAAPARGEARSAEFKRQLVEGRAALETAYRVRPNPPAL
                     LKRQAALVDRVLREVWAAAAMPSGLTLVAVGGYGRGTLFPYSDVDVLVLLPEEADDGD
                     RERVSQLVGRLWDIGVELGHSVRTIEQCLSEARQDITVQTNLLEARWVAGSKPLFRRF
                     DSTIHAHLEVQAFFEAKLLEQQQRHSRFNDTAYNLEPNLKEGPGGLRDLTTILWISAA
                     SGTGRSWKDLSRHGVITAEEAQRIGRDERALQDLRIRLHYHSRRREDRLVFDLQTPLA
                     QELGFADTPAKRSSEQLMQRYYRTARAVTQLNEIILQNLRAIVFPAPDLRPRRLDERF
                     EARHELLAACDPAVFEREPQAILEVFELMQRHPELKGIEAGTLRALWRSAKGIDALFR
                     RDPGNRERFLGMFRYGAGLTHALRRMNRYDVLGRYLPPFGRIVGQMQHDLFHVYTVDE
                     HILMVVRNLRRFAVTDFAHEYPLCSRLMTEFERPEVLYLAGLFHDIAKGRGGDHSTLG
                     GVDARRFCRNHGLGEEDTELIAWLVERHLHMSSVAQKHDLADAAVIAEFARVVQTDRR
                     LVALYLLTVADIRGTSPKVWNAWKGKLLEDLFWSTRRFFGGDTDPAGSRLQARQKLAL
                     EKLRLYAVPDGAHAKLWSRLDTTYFLRHDAREIAW"
     gene            complement(1993..2793)
                     /gene="map"
                     /locus_tag="VD791_02350"
     CDS             complement(1993..2793)
                     /gene="map"
                     /locus_tag="VD791_02350"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HXV06842.1"
                     /translation="MSVTIKSPEDIDKMRVAGRLASEVLDFITPHVKAGVTTEELDAL
                     CHQYMVREQKTVPAPLNYAPPGYPPYPKSVCTSVNHVVCHGVPGNKRLKAGDIVNIDV
                     TVIKDGFHGDTSRMFLVGEPSIQARRLVDITYECMWLGIRAVRPGACLGDIGHAIQTH
                     AERHGFSVVREFCGHGIGRRFHEEPQVLHYGRPGTGLKLQPGMTFTVEPMINAGKAGI
                     RQLGDGWTIVTKDHSLSAQWEHTVLVTPGGYEVLTLSEGTPQPAALAA"
     gene            3144..4850
                     /locus_tag="VD791_02355"
     CDS             3144..4850
                     /locus_tag="VD791_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HXV06843.1"
                     /translation="MQEFVARFRPFFVYAAGFSLVINLLMLMPALFMLQVYDRVITSH
                     SRETLAMLFLLVTAALLFMAYLDLIRSRLLAGSAVALEKLLGPRVLGSMLRRAADPRP
                     NESAANESTHGLRDVAAMRAFLTGPGIVALFDAPWVPVYALIIALFHPLLGAIAVCGA
                     LLLFAMAYLNERLSRQPLEAMQAESRKAGRFVDQGVANAEVAGALGMADNLARGWQKL
                     SRRVLTFQLEASRAGSLFSSAGKFLRQFLQAVMLAAGAWLVIEQQATSGVMIAATILL
                     GRALAPVEAAIAGWKGLVDARAAYGRVSKGLNAEAQGEPPTALPAPRGALSVERVVFA
                     FRGQDRPVIRQVTFELAPGEALAIVGPSAAGKSTLARVIVGLWKPMAGAVRLDSADIA
                     SWPRDRLGPYVGYLPQDVELFAGTVSENIARMGEIDSPAVIEAATRANAHELILRLPK
                     GYDTPIGEGGSFLSAGQRQRIALARALYGDPRLVVLDEPNSNLDADGEAALLEAVRRL
                     KAERRTVLVVTHRPQLLAAMDKVMLLREGVIEKMGLMAEVMGGVRPVHSEAGPAALAA
                     RG"
     gene            4858..6192
                     /locus_tag="VD791_02360"
     CDS             4858..6192
                     /locus_tag="VD791_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HXV06844.1"
                     /translation="MAGALSKLLLPAVNVDNAEDPRPVARAGLVAVALLVLAVSAWMA
                     LAPIGGAAIAPGVVKVDMNRKTVQHQEGGIVGEILVRDGDKVKAGQPLLVLKDVRVDA
                     SNELVQTQLDAELAKAARFTAEQAWAADIAFPSELKARAGDSRVAEIVQREKSLFRAR
                     RSAYDNQLALIKREIAETQAEIRARDRQLEADAQAIRLQREELAANEGLLDQGFVSKT
                     RLITLQRSVAELEGRHGESEAERSRARQKVSDLELRAENLRSTLMQEAANELRQTTGQ
                     IFDLRERLRPTRDAEERQRITAPIAGEVVALRVTSVGAVIAPREPILDLVPENADLVI
                     EARLRPEDISFVRSDAPADVRLTAFRQRITPTVKGTVTYVSADRLEDKDAKMSYYVAH
                     VRVTPRALREAGDLQLQAGMPAEVFIRTTSRTALQYLLDPISGFLQKSLREH"
BASE COUNT          958 a         2108 c         2280 g          951 t
ORIGIN      
        1 ccaggcgatc tcgcgcgcgt cgtggcgcag gaagtaagtc gtgtcgagcc ggctccagag
       61 cttcgcgtgc gcgccgtcgg gtacggcgta caggcgcagc ttctccagcg cgagcttctg
      121 ccgggcctgc agacggctgc ccgcggggtc ggtgtcgccg ccgaaaaagc gccgggtgga
      181 ccagaacagg tcctccagca gcttgccttt ccaggcgttc cagaccttcg ggctggtgcc
      241 gcggatgtcg gcaacggtca gcaggtacag cgccaccagg cggcgatcgg tttgcaccac
      301 gcgcgcgaat tcggcgatca cggcggcgtc ggccagatcg tgcttctgcg ccaccgacga
      361 catgtgcaga tggcgctcca ccagccaagc gatgagctcg gtgtcctctt cgcccagccc
      421 gtggttgcgg cagaaacgcc gcgcatcgac gccgccgagc gtcgagtgat cgccgccgcg
      481 gcccttggcg atgtcgtgga acaggccggc caggtacagc acttcgggcc gctcgaattc
      541 ggtcatcagg cggctgcaca gcgggtactc gtgcgcgaag tcggtgacgg cgaagcgccg
      601 caggttgcgc accaccatga ggatgtgctc gtccaccgtg tagacgtgga acaggtcgtg
      661 ctgcatctgg ccgacgatgc ggccgaacgg gggcaggtag cgcccgagca cgtcgtagcg
      721 gttcatccgc cgcagcgcgt gcgtgagccc ggcaccgtag cggaacatgc cgaggaagcg
      781 ctcccggttc cccgggtcgc gccggaacag cgcgtcgatg cccttggccg agcgccacag
      841 cgcgcgtaac gtaccggctt cgatgccctt cagctccgga tggcgctgca tcaactcgaa
      901 cacctccaag atggcctggg gctcgcgctc gaacaccgcc gggtcgcagg ccgccagcag
      961 ctcgtgacgg gcctcgaagc gctcgtcgag ccgccgtggc cgcagatccg gggccgggaa
     1021 gacgatcgcg cgcaggttct gcaggatgat ctcgttgagc tgcgtgactg cgcgcgcggt
     1081 gcggtagtag cgctgcatca gctgctcgct ggagcgcttg gcgggggtat cggcgaagcc
     1141 cagctcctgc gccagcggcg tctgcaggtc gaacaccagc cgatcctcgc gccggcgcga
     1201 atggtagtgc aggcggatgc gcaagtcctg cagcgcacgc tcgtcgcgcc cgatgcgctg
     1261 cgcttcctcg gcggtgatca ccccgtgccg cgaaaggtcc ttccagctgc ggccggtgcc
     1321 gctggcggcg ctgatccaca ggatggtggt gaggtcgcgc aggccaccgg gaccctcttt
     1381 gaggttgggt tcgagattgt aggcagtgtc gttgaaacga ctgtggcgct gctgctgctc
     1441 gagcagcttg gcctcgaaga aagcctggac ctcgaggtgc gcgtggatgg tggagtcgaa
     1501 gcgccggaac agcggcttgc tgcccgccac ccagcgcgct tccagcagat tggtctgcac
     1561 cgtgatgtcc tgccgcgcct cgctcaggca ctgctcgatg gtgcgcacgc tgtgcccgag
     1621 ttcgacgccg atatcccaaa gccggccgac cagttgcgag acccgctcgc gatcgccgtc
     1681 gtccgcctcc tcgggcagga gcaccaggac gtcgacatcg gagtagggga acagcgtgcc
     1741 gcggccgtag ccgcccaccg ccaccagcgt caggccggaa ggcatcgccg ctgcggccca
     1801 cacttcgcgc agcacgcggt ccaccagcgc cgcctggcgc ttgagcaggg caggcgggtt
     1861 gggacgcacg cggtaggcgg tttcgagcgc ggcacgcccc tcgaccagct ggcgcttgaa
     1921 ttcggcggag cgcgcctcgc cccgggcggg ggcggcggcg ggaacggagg actgcaccgg
     1981 gcgcagcccc tgctatgccg cgagcgcagc cggctgcggc gtgccctcgg acaatgtgag
     2041 cacctcgtag ccgccgggcg tgaccagcac ggtgtgctcc cactgggccg acaggctgtg
     2101 gtccttggtg acgatggtcc agccgtcgcc gagctggcgg atgccggcct tgccggcgtt
     2161 gatcatcggc tcgaccgtga aggtcatccc cggctgcagc tttagcccgg tcccgggccg
     2221 gccgtaatgc agcacctgcg gctcctcgtg aaagcgccgg ccgatgccgt gcccgcagaa
     2281 ctcgcgcacc acgctgaagc cgtggcgctc ggcgtgcgtt tggatggcgt gaccgatgtc
     2341 acccaggcag gcgccgggac gcaccgcgcg gatgccgagc cacatgcact cgtaggtgat
     2401 gtcaaccagc cggcgcgcct ggatcgaagg ctcccccacc aggaacatgc ggctggtgtc
     2461 gccgtggaac ccgtccttga tcacggtgac gtcgatgttg acgatgtcgc cggccttcag
     2521 ccgcttgttg cccggcacgc cgtggcacac cacgtgattg accgaggtgc agaccgactt
     2581 gggatagggg gggtagccgg gcggagcgta gttgagcggc gcgggcaccg tcttctgctc
     2641 ccgcaccatg tactggtggc acagggcatc cagctcctcg gtggtcacgc ccgccttgac
     2701 gtgcggcgtg atgaagtcga gcacctcgct ggccaggcgg ccggcgacgc gcatcttgtc
     2761 gatgtcctcg ggggacttga tggtgacgga catggatgag cggggcgcgc agggaatcgg
     2821 cccgacggga aacgaccccg gtcgcacgaa cgacccccgg tcgcacaaac gaccccgatc
     2881 gcacaaacga ccccggggca cacgaatccg gcgggcgcgc ccctctgttt gagtgctgcg
     2941 attctgcccg tgaaatgtta gcacagctca ccgcgcgtcc cggcttgacg ggcgccccgg
     3001 ccgggcgggc ccgccccgcg gggccggatc cgggggcggc gccgccggcg tttgacgatt
     3061 gctcatattt gccgctggca gaggcacgat gttagctttc cgaggcttac cggtccgcaa
     3121 cccggccggc ccgacccccg tccatgcaag agttcgtcgc gcgcttccgc ccgttcttcg
     3181 tctacgcagc cggtttcagc ctggtcatca acctgctgat gctgatgccc gcgctgttca
     3241 tgctgcaggt ctacgaccgc gtcatcacca gccacagccg cgagacgctg gcgatgctgt
     3301 tcctgctggt gaccgcggcc ctgctgttca tggcctacct cgacctgatc cgctcgcgcc
     3361 tcctggccgg gtcggcggtg gcgctggaga agctgctcgg gccgcgggtg ctggggtcga
     3421 tgctgcgccg ggcggccgac ccccgcccca acgagtccgc agccaatgag tcgacgcacg
     3481 gcctgcgcga cgtcgccgcg atgcgcgcgt tcctcaccgg ccccgggatc gtggcgttgt
     3541 tcgacgcgcc gtgggtgccg gtctacgcgc tcatcatcgc gttgttccat ccgctgctgg
     3601 gcgcgatcgc cgtgtgcggg gcgctgctgc tgttcgcaat ggcctacctg aacgaaaggc
     3661 tgtcgcgcca gccgctggaa gcgatgcagg ccgagtcgcg caaggccggc cgcttcgtgg
     3721 accagggcgt ggccaacgcc gaggtggccg gcgccttggg gatggcggac aaccttgcgc
     3781 gcggttggca gaagctgagc cggcgcgttc tcacgttcca gctcgaagcg agccgggcgg
     3841 ggagcctgtt ctcgagcgcc ggcaagttcc tgcgccagtt cctgcaggcg gtcatgctgg
     3901 ccgcgggcgc ctggctcgtc atcgagcagc aggccaccag cggggtgatg atcgccgcca
     3961 ccattctgct cggccgggcg ctggcgccgg tcgaagcggc catcgccggc tggaaggggc
     4021 tggtggatgc gcgcgccgcc tacgggcgcg tgagcaaggg gctgaacgcc gaagcgcagg
     4081 gcgagccgcc gaccgcgttg cccgctccgc gcggtgcgct gtccgtcgag cgcgtggtgt
     4141 tcgccttccg cggacaggac cggccggtga tcaggcaggt gacgttcgag ctcgcccccg
     4201 gcgaagccct ggcgatcgtc ggtcccagcg cggcgggcaa gtccacgctg gcgcgcgtga
     4261 tcgtgggctt gtggaagccg atggccggcg ccgtgcggct ggacagcgcc gacatcgcga
     4321 gctggccccg cgaccggctc ggtccctacg tcggctatct gccgcaggac gtcgagctgt
     4381 tcgccggcac ggtgagcgag aacatcgccc gcatgggcga gatcgactcg ccggcggtca
     4441 tcgaagcggc cacgcgggcc aacgcccacg agctgatcct gcgcctgccc aaaggctacg
     4501 acacgccgat cggcgagggc ggctcgttcc tgtcggccgg gcagcggcag cgcatcgcgc
     4561 tcgcgcgcgc gctgtatggc gatccgcggc tggtggtgct ggacgagccg aactcgaacc
     4621 tggacgcgga cggcgaagcg gcgctgctgg aggcggtgcg caggctcaag gcggaacgca
     4681 ggacggtgct ggtggtgacg catcgcccgc agctgctcgc cgccatggac aaggtcatgc
     4741 tgctgcgcga gggcgtgatc gagaagatgg gactgatggc ggaagtcatg ggcggggttc
     4801 ggccggtgca ctcggaagcg ggaccggcgg ccttggcagc cagagggtag gcgaaccatg
     4861 gcaggcgccc tctcgaagct gttgctgccg gccgtgaacg tggacaacgc cgaggatccg
     4921 cggccggtgg cgcgcgctgg cctggtcgcc gtggcgctgc tggtgctcgc ggtcagcgcc
     4981 tggatggcgc tggcgccgat cggcggcgcg gcgatcgcgc ccggcgtggt caaggtggat
     5041 atgaaccgca agacggtcca gcaccaggaa ggcggcatcg tcggcgagat cctggtgcgc
     5101 gacggcgaca aggtgaaggc aggtcagccg ctgctggtac tgaaggacgt gcgcgtggat
     5161 gccagcaacg agctggtgca gacgcagctc gacgccgagc tggcgaaggc cgcacgcttc
     5221 acggccgaac aggcgtgggc cgcggacatc gcttttccgt cggagctgaa ggcgcgggcc
     5281 ggcgactcgc gcgtggcgga gatcgtccag cgcgagaagt cgctgttccg tgctcggcgc
     5341 tcggcctacg acaaccagct cgccctgatc aagcgcgaga tcgccgagac gcaagccgag
     5401 atccgggcgc gggaccgcca gctcgaggcg gacgcgcagg cgatccggct gcagcgcgag
     5461 gagctggctg cgaacgaagg gctgctcgat cagggcttcg tcagcaagac gcggctgatc
     5521 actctgcagc gcagcgtggc cgagctggaa gggcggcacg gcgaaagcga ggcggagcgc
     5581 tcgcgcgcgc ggcagaaggt gtccgacctc gagctgcgcg ctgagaatct gcgcagcacc
     5641 ttgatgcaag aggcggcgaa cgagctgcgc cagacgaccg ggcagatctt cgacctgcgc
     5701 gagcggctgc gtccgacccg ggatgcagag gaacgccagc gcatcaccgc cccgatcgca
     5761 ggagaagtag tggcgctgcg agtcaccagc gtcggcgcgg tgatcgcccc gcgcgaaccg
     5821 atcctcgatc tcgtgccgga gaacgcggac ctggtcatcg aagcgcgcct gcggcccgag
     5881 gacatcagct tcgtgcgcag cgacgccccc gcggacgtgc gcctgacggc gttccgacag
     5941 cgcatcacgc ccacggtgaa ggggacggtg acctacgtgt cagccgaccg cctggaggac
     6001 aaggacgcga agatgtctta ctacgtggcg cacgtacgcg tcaccccgcg ggcgctgcgc
     6061 gaagccggag atctgcagct gcaggcgggc atgccggccg aagtgtttat ccgtaccacc
     6121 tcgcgcactg ccctgcagta cctgctggat ccgatcagcg gcttcctgca gaaatccctg
     6181 cgcgagcatt gaccgcctaa gctttggcgg cgatcgcctc gacctcgcac agatacggcg
     6241 gcagggtcag ccccgagatg aacaccaagg tctgggcggg tcgcgtgtca ccgaggt
//
LOCUS       DASPGE010000076         4478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_610921, whole genome shotgun sequence.
ACCESSION   DASPGE010000076 DASPGE010000000
VERSION     DASPGE010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610921"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VD791_02365"
     CDS             complement(<1..500)
                     /locus_tag="VD791_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M29"
                     /protein_id="HXV06845.1"
                     /translation="MLRERIEGRWIDCFAEVFGRCGLAGGEAVAVLSETQSRQVNVEL
                     AELALLRLGCRTFQLVVPTARLTAPAPVRSTGASNALQGLAPAVDALAACTMVVDLTV
                     EGLLHAAELPRILAGGARVLMVSNEHPEALERLMPDPALEPKVRAGMRLLKAARQMRV
                     TSPAGT"
     gene            complement(532..1080)
                     /locus_tag="VD791_02370"
     CDS             complement(532..1080)
                     /locus_tag="VD791_02370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010121925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXV06846.1"
                     /translation="MKPLRIVLAAAFALPLAAESAGAADPENTLYLDTKDGRVVIELR
                     PDLAPKHVARIKELVRKGFYDGLVFHRVIEGFMAQTGDPLGNGTGGSGTKVPAEFSKQ
                     AFERGTVGMARSRDPNSGDSQFFICFAPAPFLNGQYTVWGQVTQGMDVVDKIKKGDSA
                     ANGAVQSPDKIVRMQVAADVKQ"
     gene            1262..1909
                     /gene="pdxH"
                     /locus_tag="VD791_02375"
     CDS             1262..1909
                     /gene="pdxH"
                     /locus_tag="VD791_02375"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HXV06847.1"
                     /translation="MSHKKHIAELRREYARESLNDDDVARDPIAQFARWFQEALNSGF
                     TEPNAMTLATAGADGRPSARIVLLKDYDERGFTFFSNYESRKGEELASNPQAALLFHW
                     VELERQVRIEGRVERVAPAESDEYYRGRPLGSRLGAWASPQSRVLARREDLEARLAEV
                     GSRYGQDPPRPPHWGGYRLAPDLLEFWQGRPNRLHDRVRYARQGSASWRIERLAP"
     gene            2004..2270
                     /locus_tag="VD791_02380"
     CDS             2004..2270
                     /locus_tag="VD791_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06848.1"
                     /translation="MLTIQDCIALSELTAEEIRAIAEHEHIPEMAAIELGNYLVHTPS
                     GEKRIKAMIADDLEHARHEGRLERVLALKLCLRHFLEQHAAARE"
     gene            complement(2292..2663)
                     /locus_tag="VD791_02385"
     CDS             complement(2292..2663)
                     /locus_tag="VD791_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06849.1"
                     /translation="MPGNFRPFAYALAAALIAGCSTLKVGRDFDYNAFASRVELGTTT
                     AAQVMDWLGAPAGQGVEVGTDRTRLDVWNYYYGTGKIPSGSDTSFKLLQVKLNPEGKV
                     AAYVWTGDMAGAPVEDKSSGK"
     gene            complement(2725..3900)
                     /locus_tag="VD791_02390"
     CDS             complement(2725..3900)
                     /locus_tag="VD791_02390"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HXV06850.1"
                     /translation="MNDPVVQQLQRALGTHGRALLGQPAQLEQLLTGVAATFPGKVKA
                     LLILLDKKAVAFLIDWAEQARPGKLGFEQLRQQMVAKFEQANVLNAAAASWAFDAWAA
                     ALGIAPGALAAQAAPRAVAVSAAAAPSAGGAPAPRNVYAPPGAPVEDPRQGLEDEHDF
                     IEGGRAVPAGHGVSWLGEGWRLFKESPLIWIVNVILFLVITVAVQVVPIVGGIAGALL
                     APVLGAGLMIGAHALRHGETLEVGHLFAGFRQNTGALVLVGVVYLVGVIAIVVVAMLL
                     MGASFVGLMAGGGEPVLGPGLILAILIGVALAIPLAMAYWFAPALVALNGLSAIEAMK
                     MSFWGCLKNILPFLVYGLVALVAAIVATIPLALGWLVLGPVLAASMYASYRDIFYQE"
     gene            4132..>4478
                     /locus_tag="VD791_02395"
     CDS             4132..>4478
                     /locus_tag="VD791_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06851.1"
                     /translation="MWLNLLIAAVLLVGMIVGLYLLPRHLKGERIGFPLLARAAAQKV
                     DSGDDLERFMAAYRRDKAQSGATTTQPGGAAQAPAPRSAGRPPTLAVATVKPKGAFLA
                     GPNKLLYLVLRTA"
BASE COUNT          719 a         1594 c         1534 g          631 t
ORIGIN      
        1 tcggttccgg cgggcgaggt cacccgcatc tgcctggccg ccttgagcaa gcgcatgccg
       61 gcgcgcacct tgggttcgag cgccgggtcc ggcatcaggc gctcgagggc ctcggggtgc
      121 tcgttcgaga ccatcagcac gcgcgcgccg cccgccagga tgcgcggcaa ctcggcggcg
      181 tgcaacaggc cctcgacggt cagatccacc accatcgtgc acgcagcgag cgcgtccacc
      241 gcgggggcga gtccctgcag tgcgttgctc gccccggtcg agcgcaccgg ggcgggagcg
      301 gtcagccgcg cagtcgggac gacgagttgg aaggttcggc agcccaggcg cagcaacgcc
      361 agctcggcca gctcgacatt gacctggcgc gactgagtct cggacagcac cgcgaccgcc
      421 tcgccccccg ccagcccgca tcggccgaaa acctccgcga agcagtcgat ccatctgccc
      481 tcgatgcgct cgcgcagcat ggcccgcctc ccccgccagc gaccgcagcg gtcactgctt
      541 cacatcggca gccacctgca tgcgcacgat cttgtccggg ctctgcacgg cgccgttggc
      601 cgcgctatcg cccttcttga tcttgtccac cacgtccatg ccctgcgtca cctggcccca
      661 tacggtgtac tggccgttca ggaagggcgc gggcgcgaag cagatgaaga actgcgaatc
      721 gccgctgttg gggtcgcgcg agcgcgccat gccgacggtg ccgcgctcga acgcctgctt
      781 ggagaactcg gccgggacct tggtccccga gccgccggtg ccgttgccga gcggatcgcc
      841 ggtctgcgcc atgaagccct cgatcacgcg gtggaacacc agcccgtcgt agaagccctt
      901 gcgcaccagc tccttgatgc gcgccacgtg cttgggcgcg aggtccgggc gcagctcgat
      961 caccacgcgg ccgtccttgg tgtccagata cagcgtgttc tcggggtccg cggcgccggc
     1021 cgattcggcg gcgagcggca gggcgaaggc ggcagcgagc acaatgcgca ggggtttcat
     1081 gggtgtcaga gtcggtccgg tgggtggatg gagcgcccga tgggtggatg aagcgcccga
     1141 tgggtggatg aagcgcgtag tgtaccggtt ccggctgtag aatcgcgggc ggaacgcccc
     1201 catcgatgcc gatcccggcg gtgcgccgtc cgggcgcgcc gtgcgggtgc cgacccgacc
     1261 catgtcacac aagaagcata tcgccgagct gcgcagggag tatgcgcgag agagcctgaa
     1321 cgacgacgac gtcgcccgcg acccgatcgc gcagttcgcg cgctggttcc aggaagcgct
     1381 caactccggt ttcaccgagc ccaacgccat gacgctcgcc accgccggcg cggacgggcg
     1441 gccctcggcg cgcatcgtcc tgctcaagga ttacgacgag cgcggcttca cgttcttcag
     1501 caactacgaa agccgcaaag gcgaggagct ggccagcaac ccgcaggcgg cgttgctgtt
     1561 ccactgggtg gaattggagc ggcaggtgcg catcgagggc cgcgtcgagc gcgtcgcgcc
     1621 ggccgagtcg gacgagtact atcgcggccg gccgctcggc agccggctcg gcgcgtgggc
     1681 ctctccgcag agccgcgtgc tggcgcgccg cgaggatctg gaggcgcggc tggccgaggt
     1741 cggcagccgc tacggccagg acccgccacg cccgccgcat tggggcggct accgcctggc
     1801 ccccgacctg ctggagttct ggcaggggcg tcccaaccgc ctgcacgacc gcgtgcgcta
     1861 cgcacgtcag ggctcagcga gctggcgcat cgagcggctg gcgccgtgac ccgccggcgg
     1921 cgccgcgcag cggtccttgc gctggatcaa gacggtccgc gcgagattgc ggtctgcttg
     1981 cagcgtgcgg gccggaggaa accatgctca ccattcaaga ctgcatcgcg ctgtccgagc
     2041 tgaccgcgga ggagatccgg gcgatcgccg agcacgagca catcccggag atggccgcga
     2101 tcgagctcgg caactatctg gtgcacacgc ccagcggaga gaaacgcatc aaggcgatga
     2161 tcgccgacga cctcgagcac gcgcgccacg aaggccgcct ggagcgcgtg ctggcgctca
     2221 agctgtgcct caggcatttc ctggagcagc acgccgcggc acgcgagtag ccgcggcgcg
     2281 cggcgccggc gctacttgcc ggacgactta tcctcgaccg gcgcgccggc catgtcgccg
     2341 gtccacacgt aggcggcgac cttgccctcc gggttgagct tgacctgcag cagcttgaag
     2401 ctggtgtcgg agccgctggg gatcttgccg gtgccgtagt agtaattcca cacgtccagg
     2461 cgagtgcggt cggtgcccac ttccacgccc tggccggccg gagcgccgag ccagtccatc
     2521 acctgcgcgg cggtggtggt gccgagctcc acgcgcgaag caaaggcgtt gtagtcgaag
     2581 tcgcgcccca ccttgagcgt gctgcagccg gcgatcagag ctgccgcgag cgcatacgcg
     2641 aacggtcgga agttgcctgg catggtctcc tcctgtcttg ggcccgggcg ggattctagc
     2701 aggggcgccc ggccggcgcg cgcgttattc ctggtagaag atgtcgcgat agctcgcgta
     2761 catggatgca gccagcaccg gcccgagcac cagccagccc aacgcgagcg gaatggtggc
     2821 gacgatcgcc gccaccagcg ccaccaggcc gtacaccaag aatggcagga tgttcttcag
     2881 gcagccccag aagctcatct tcatcgcttc gatcgcggac agcccgttca aggccaccag
     2941 cgccggcgcg aaccagtagg ccatcgccag cggaatggcg agcgcaacgc cgatcaggat
     3001 ggcgaggatc agtcccggtc ccagcaccgg ctcgccgccg cccgccatca gaccgacgaa
     3061 gctggcgccc atcagcagca tcgccacgac cacgatcgcg atcaccccca ccaggtacac
     3121 cacaccgacc agcaccagcg cgccggtgtt ctgccgaaag ccggcgaaca ggtgtccgac
     3181 ctcgagggtt tctccgtggc gcagcgcgtg cgcgccgatc atcaggccag cgcccagcac
     3241 cggggcgagc aatgcgcccg caatgcctcc gacgatgggc accacctgca ccgcgacggt
     3301 gatgaccagg aacaggatca cgttgacgat ccagatcagc gggctctcct tgaacaaccg
     3361 ccagccctcg ccgagccagg acacgccgtg accggccggc acggcgcgcc cgccctcgat
     3421 gaagtcgtgc tcgtcctcga gcccctgacg cggatcctcg accggcgcgc cgggcggggc
     3481 atagacgttg cgcggggcgg gggctccccc ggccgacgga gcggccgccg ccgaaacggc
     3541 aaccgccctc ggcgccgctt gcgcggcgag cgcgccgggc gcgatgccca gggccgcggc
     3601 ccaggcgtcg aaggcccagg aggctgcggc cgcgttcagg acgttggcct gctcgaactt
     3661 cgccaccatc tgctggcgta gctgctcgaa gccgagcttg cccgggcgcg cctgctccgc
     3721 ccagtcgatc aggaacgcca ccgctttctt gtcgagcagg atgagcagcg ccttcacctt
     3781 gccggggaag gtcgcggcaa cgccggtgag gagctgttcg agctgtgccg gctgcccaag
     3841 gagggcgcgg ccatgcgtgc ccagggcacg ctgcaactgc tgcaccactg gatcgttcaa
     3901 ggccctctcc tcgatcgcgc aatcgctccg ccgcgcgcat gccgcaggca tgcgtagccg
     3961 gtgctttcgc gagacgaatc aacgcctgta gcgattgtca aagcaacgtg catgccacgg
     4021 cggagggttc acaaacgcgc agcgcgcttg gcacggggcg tgcaactcgg cacaccgtcg
     4081 cgcgcagccg cgcacccccg gggcgggcaa ccccttggcc ttcgacgacc catgtggctc
     4141 aacctgctga tcgcggccgt cctgctggtc ggcatgatcg tcggcttgta tcttctgccg
     4201 cgccacctca agggcgagag gatcggcttt cctctgctcg cgcgcgccgc cgcgcagaag
     4261 gtcgacagcg gcgacgatct cgagcggttc atggccgcct atcgacgcga caaggcgcag
     4321 agcggtgcga cgaccaccca gcccggaggg gcggcccaag caccggcacc gcggtcggcc
     4381 ggacgaccac cgacgctggc ggtagccacc gtcaagccga agggggcgtt tctcgcgggg
     4441 ccgaacaagc tgctgtacct ggtgctgcgc accgcctt
//
LOCUS       DASPGE010000077         3434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_727847, whole genome shotgun sequence.
ACCESSION   DASPGE010000077 DASPGE010000000
VERSION     DASPGE010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727847"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VD791_02400"
     CDS             complement(<1..773)
                     /locus_tag="VD791_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06852.1"
                     /translation="MARSLKEPKIVRPDEIDPHHNWGRPIQAPGHTQVDFEERVNFRR
                     LHDYRLARVRAALSMSGLGALLCFDQANIRYTTSTVIGEWARDKLTRYSLLTGNGDPY
                     IWDFGSAAKHHRLYAPWLHSDHCRAGLLGMRGAVGADIALFREAAQEIKALLDTEGVG
                     GMPLGVDVVEPPMLFELQRLGLEVRDGQQAMLQAREVKSIDEITLLNMASAMVDGVYQ
                     DIAEALKPGVKESEIVAMATARLYEMGSDCVEAINSISG"
     gene            1190..1729
                     /locus_tag="VD791_02405"
     CDS             1190..1729
                     /locus_tag="VD791_02405"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HXV06853.1"
                     /translation="MYTPWTGERWIFRPASGWLRIRRNEDLISLVLCVAVVFVGIMLI
                     VQMLDQYSRAKTMAAWPTALGRILSSGIEPVASDGELRWRPAVRYMYEVRGQSVISTG
                     LSLATARDDYSEAQARQMALPYPPNTTVVVFYNPQHISDAVLDHSLPRFAWLSLFAGL
                     ILVSAGGARLLIAHRVFWR"
     gene            complement(1734..3353)
                     /locus_tag="VD791_02410"
     CDS             complement(1734..3353)
                     /locus_tag="VD791_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV06854.1"
                     /translation="MTAPALRVEGLTVRLPPGADRTHAVTGVSLEVRQGELMCLVGES
                     GSGKSIVAHTVMGLLPGNVRATAGRVLLGEEDLLRATPERLRRLRGEQMAMIFQEPMT
                     ALNPVMRCGAQIDELLAQHTALRSDERRARVLRMLEHVRLPDPAAIHAAYPHQLSGGQ
                     RQRIMIAMALILQPRLLIADEPTTALDVTTQAEILRLMRELQRESGTAVLFITHDFGV
                     VAEIADRVSVLKLGELVEAGDAQAVLSRPRHAYTRMLLASVPALVPRKAPLPDGTGVQ
                     LRTRGLTKVFDAGGWFGRRRRVTAADAVEIDLLRGETLGIVGESGSGKSTVARLLAHL
                     IEPTGGEIELDGEPVVRLRGDAARRFRRKVQIVFQDPYRSLNPRRTVGAAVAEGPLNF
                     GTGRKAAWLRAGELMELVKLSPAALQRYPHEFSGGQRQRICIARALAVDPEILIADEA
                     VSALDVSVQAEILKLLDEIQRRLRLTLLFITHDLRVAAQICNRVVVMRGGRVVEQGPV
                     AEVFERPRDAYTRALIDAAPGRGFAFGEEVA"
BASE COUNT          515 a         1219 c         1159 g          541 t
ORIGIN      
        1 tcccccgaga tcgagttgat cgcctccacg caatccgacc ccatctcgta cagccgcgcc
       61 gtggccatcg ccacgatctc gctctccttg acgccgggct tgagcgcctc ggcgatgtcc
      121 tggtagacgc cgtccaccat cgccgaggcc atgttcagca gcgtgatctc gtcgatgctc
      181 ttcacctcgc gcgcctgcag catcgcctgc tggccgtcgc gcacttccaa ccccaggcgc
      241 tgcagctcga acagcatcgg cggctcgacc acgtccacgc ccagcggcat gccgcccacg
      301 ccctccgtgt cgagcagcgc cttgatctcc tgcgccgcct cgcggaacag cgcgatgtcc
      361 gcgccgaccg cgccgcgcat gccgagcagc ccggcgcggc agtggtccga gtgcagccat
      421 ggcgcataca ggcggtgatg cttggccgcc gagccgaagt cccagatata aggatcgcca
      481 ttgccggtga gcagcgagta gcgcgtgagc ttgtcgcgcg cccattcgcc gatcaccgtg
      541 ctggtggtgt agcggatgtt ggcctggtcg aagcagagca gggcacccag gcccgacatg
      601 gacagcgccg cacgcacgcg cgccaggcga tagtcgtgca ggcggcggaa attgacgcgc
      661 tcctcgaagt ccacctgcgt gtgcccgggc gcctggatcg gccggcccca gttgtggtgc
      721 ggatcgatct cgtcgggccg cacgatcttc ggctccttca ggctgcgcgc catgggtttc
      781 ctcccctttc gttatagtgc cggcccgctc gccgggtgcg acaatgtcag ggaattctgt
      841 aacagcggga ggccagcgta aaggatcgcc ggggaacgga tgcaccaggg ctgtgcggcc
      901 gcggcggcgg ttttgaggat cgtttgacac taatgtctga cctgtccgca actggctaca
      961 cttggcgccg aattcgaacg ggcgaagcca atagcaacgg cggcgggccg ggatccggca
     1021 agtcgcttca ccccgcggat ttgcagggct tttttggagt ttccagccgg aaggacggtc
     1081 ccgcgacggc gctgtcgaac gacttggcgg gcgatgccag cccgccgtcc gacgcagccc
     1141 accagcaggc cgtgtcccgg gcgaagtgaa ggcgatcgga ggtttgagcg tgtatacccc
     1201 ctggaccggt gagcggtgga tctttcgacc cgccagcgga tggttgcgca tccggcgcaa
     1261 cgaggacttg atctcgctgg tgctgtgcgt ggcggtcgtc ttcgtcggca tcatgctgat
     1321 cgtgcagatg ctggatcagt acagccgcgc caagacgatg gccgcctggc ccacggccct
     1381 cggccgcatc ctgtcttccg gcatcgagcc ggtggcctcc gacggcgagc tgcgctggcg
     1441 gccggcggtg cgctacatgt acgaggtgcg cgggcagagc gtgatcagca ccggcctgtc
     1501 gctcgccacc gcgcgcgacg actacagcga ggcgcaggcg cgccagatgg cgctgcccta
     1561 tccgccgaac accacggtgg tcgtgttcta caatccccag cacatcagcg acgcggtgct
     1621 cgatcactcg ctgccgcgct tcgcgtggct cagcctgttc gcggggttga tcctggtgag
     1681 cgccggcggc gcccggctac tgatcgcgca ccgcgtgttc tggcgctgac cgattaggca
     1741 acctcctccc cgaacgcgaa gcctcgcccc ggcgccgcgt cgatgagcgc gcgcgtataa
     1801 gcgtcgcgcg ggcgctcgaa cacctccgct accggcccct gctccaccac gcgaccgccg
     1861 cgcatcacca ccacgcggtt gcagatctgc gccgccacgc gcagatcgtg ggtgatgaac
     1921 agcagcgtaa gccgcaggcg gcgctggatc tcgtccagca gtttcaggat ctcggcctgc
     1981 accgagacgt ccagcgcgga caccgcttcg tccgcgatca ggatctcggg gtccaccgcc
     2041 agcgcgcgcg cgatgcagat gcgctgccgc tgaccgccgg agaactcgtg cggatagcgc
     2101 tgcagggctg cgggcgagag cttgaccagc tccatcagct cgcccgcgcg cagccaggcc
     2161 gccttgcggc ccgtgccgaa gttgagcgga ccctcggcga cggcggcgcc caccgtgcgg
     2221 cgtggattga gcgagcggta ggggtcctgg aagacgatct gcaccttccg ccggaaacgc
     2281 cgtgcggcgt cgccgcgcag gcgaacgacc ggttcgccgt ccagctcgat ctcgccgccg
     2341 gtcggttcga tcaggtgcgc gagcaggcgc gcgaccgtgg acttgccgga tccggactcg
     2401 cccacgatgc ccagcgtctc gcctctcagc agatcgatct ccaccgcatc ggccgcggtc
     2461 acgcggcgcc gcctgccgaa ccagccaccg gcgtcgaaca ccttggtcag cccgcgcgtg
     2521 cgcaattgga cccccgtgcc gtccggcagc ggcgccttgc gcggcaccag cgcaggcacc
     2581 gacgccagca gcattcgcgt gtaggcgtgg cgcgggcggc tcagcaccgc ctgcgcatcg
     2641 ccggcttcca ccagctcgcc caacttcaga accgacaccc ggtcggcgat ttccgccacc
     2701 accccgaagt cgtgcgtgat gaacagcacg gcggtgccgc tttcgcgctg cagctcgcgc
     2761 atcaggcgca ggatctcggc ctgcgtggtg acgtcgagcg ccgtggtcgg ctcgtccgcg
     2821 atcagcaggc gcggctgcag gatgagcgcc atcgcgatca tgatgcgctg tcgctggccg
     2881 ccggagagct gatgcggata ggcggcgtgg atcgcggccg gatcgggcag gcgcacgtgc
     2941 tccagcatgc gcagcacgcg cgcgcgccgc tcgtcggatc gcagcgcggt gtgctgggcg
     3001 agcagctcgt cgatctgggc gccgcagcgc atcaccgggt tgagcgcggt catcggctcc
     3061 tggaagatca tcgccatctg ctcgccgcgc agccggcgca gccgctctgg cgtggcgcgc
     3121 aacaggtctt cttcgcccag cagcacgcgg ccggcggtgg cgcgcacatt gcccggcagc
     3181 aggcccatca cggtgtgagc gacgatcgat ttgccggaac cggactcgcc caccaggcac
     3241 atcagctcac cctgccgcac ttccaggctc acgccggtca cggcatgcgt gcgatcggcg
     3301 ccgggtggca ggcgcacggt cagcccttcg acgcgcagcg cgggtgcggt cacggtcggt
     3361 ctccacgccc gtagttggaa gactcccggg agaagcgcgg gtccagcgta tcgcgcaggc
     3421 cgtcgccgag gatg
//
LOCUS       DASPGE010000078         4265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_612065, whole genome shotgun sequence.
ACCESSION   DASPGE010000078 DASPGE010000000
VERSION     DASPGE010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612065"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1528)
                     /locus_tag="VD791_02415"
     CDS             complement(<1..1528)
                     /locus_tag="VD791_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HXV06855.1"
                     /translation="MQTATEVAAGSAAAVLSIEGDAQPRDPRYSQYRVIRRNGAVVGF
                     EPGKISVAVTKAFLAVNGSQGAASARVREQVAELTEQVVGALMRRHPQGGTLHIEDIQ
                     DQVELALMRSGEHDVARAYVLYREERARLRAQQKAAQAEPRQQTLTVVDNGVSRALDL
                     ERLQTLVADCCQGLGHAVEPGVILQAVLKDLYDGVPMDEVRKCAILSARALIEKDPAY
                     SYVTARLLLHSIQVEVLGEEATHADMRSRYAEYFAGFIERGIAAGLLDPRLRSYDLEA
                     LARALDANRDFKFGYLGLQTLYDRYFIHVDGRRIELPQAFFMRVAMGLALKETEREAR
                     AIEFYNVLSSFDFMSSTPTLFNSGTHRSQLSSCYLTTVSDDLDGIYEAIKENALLSKF
                     AGGLGNDWTPVRALGSHIKGTNGKSQGVVPFLKVVNDTAVAVNQCFAPDTPVYTADGA
                     KAIRDVRPGDLVLGKSGSYREVTRTFVYNQTDPMVAVHVKHAIEPLCVTAGHPFYAIQ
                     G"
     gene            complement(1694..2254)
                     /gene="ampD"
                     /locus_tag="VD791_02420"
     CDS             complement(1694..2254)
                     /gene="ampD"
                     /locus_tag="VD791_02420"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HXV06856.1"
                     /translation="MSAGGGAQTFDAAGRLQRARQIASPNFDERPAGEAIRLVVVHNI
                     SLPPHEFGGPGVIDLFLNRLDPGAHPFYPAIAGLRVSSHFYIRRDGELIQFVPCAQRA
                     WHAGASRWNGRERCNDFSVGVELEGSDFVPFRVAQYEALSRLTRELMAHYPIEAVLGH
                     SDIAPGRKTDPGPFFDWARFRALIRA"
     gene            complement(2310..2798)
                     /locus_tag="VD791_02425"
     CDS             complement(2310..2798)
                     /locus_tag="VD791_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06857.1"
                     /translation="MTSRVLALLFLFTLAAAADAVGSRSLLGTWEAARSPDDERLYVH
                     LKHNGRAEIVAEYDFTLPGVPGKRRGRSTTFGKWSVSGDDVVISYARVRDRLRYSDKL
                     PLAEIGLTGTSAGLKPTGKTDPKSRIRGATLWKAPHEYKRRTSEAAPAAGTTAESGTP
                     GQ"
     gene            complement(2876..4255)
                     /locus_tag="VD791_02430"
     CDS             complement(2876..4255)
                     /locus_tag="VD791_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV06858.1"
                     /translation="MGSGGRATAPQRCVLVVDDEVDILELIELTLLRMGLDVERAMSV
                     RDAAAKITAGRHYDLCLTDMRLPDGTGLEIVENITRAGLDLPVAVITAHGNTENAVAA
                     LKAGAFDYVSKPVSLDQLRTLVKSALSLPKVGETKSIELAPGEKGLLGTSPAIGHVRD
                     LIEKLARSEAPVHITGESGSGKELAARLIHQKSARRDRPFVPVNCGAIPENLMESEFF
                     GYRKGAFTGADADRDGFFQAANGGTLFLDEVADLPLAMQVKLLRAIQEKRARKVGSTQ
                     EDAVDVRIISATHQNLADCVESGKFRHDLYYRLNVIELRMPALRDMREDIPAIAGAVL
                     DKLAGQAKASLPQLEPEAMQALAQYDFPGNVRELENILERAMALCDGQKITTADLQLT
                     PPEAEEVVRTSEGSEGKWPLQEYLDRVEKEAILEALEKTRYNRTAAAKLLGITFRAMR
                     YRMERLGIN"
BASE COUNT          604 a         1471 c         1483 g          707 t
ORIGIN      
        1 ccccctggat ggcatagaaa ggatggcccg cggtcacgca cagcggctcg atggcgtgct
       61 tgacgtgcac cgccaccatc gggtcggtct ggttgtatac gaaggtgcgc gtgacctcgc
      121 ggtagctgcc gctcttgccg agcacgagat ctcccgggcg cacatcgcgg atcgccttcg
      181 ccccgtcggc ggtgtagacc ggcgtgtccg gggcgaagca ctggttcacc gcgacggcgg
      241 tgtcgttgac caccttcagg aacggcacca cgccctggga cttgccgttg gtgcccttga
      301 tgtggctgcc gagcgcccgt accggcgtcc agtcgttgcc cagaccgccg gcgaacttgg
      361 acagcagggc gttctccttg atcgcctcgt agatgccgtc gagatcgtcg gacacggtgg
      421 tcaggtagca gctggagagc tgcgagcggt gcgtgccgga gttgaacagc gtcggggtcg
      481 agctcatgaa gtcgaagctc gacagcacgt tgtagaactc gatggcgcgg gcctcgcgct
      541 cggtctcctt gagcgccagt cccatcgcca cgcgcataaa gaaggcctgc ggcagctcga
      601 ttcggcgacc gtccacgtgg atgaaatagc ggtcgtagag cgtctgcagg ccaaggtagc
      661 cgaacttgaa gtcgcggttg gcgtcgagcg cccgggcgag cgcctcgagg tcgtagctgc
      721 gcagccgcgg atccagcagg ccggcggcga tgccgcgttc gatgaagccg gcgaagtact
      781 cggcgtagcg gctgcgcatg tcggcgtggg tcgcctcctc gcccagcacc tcgacctgga
      841 tgctgtgcag cagcaaacgc gccgtgacgt agctgtaggc cgggtccttc tcgatcagcg
      901 cgcgggccga caggatggcg cacttgcgca cctcgtccat cggcacgccg tcgtacaggt
      961 ccttgagcac cgcctgcagg atcacgccgg gctcgacggc gtgccccagc ccttggcagc
     1021 agtccgcgac caaggtctgc agccgctcga ggtcgagggc gcggctcacg ccgttgtcga
     1081 ctacggtcag cgtttgctgc cggggctcgg cctgcgcggc cttctgctgc gcgcgcaggc
     1141 gcgcgcgctc ttcgcggtac agcacgtagg cgcgcgccac gtcgtgctcg ccggagcgca
     1201 tcagcgccag ctcgacctgg tcctggatgt cctcgatgtg cagggtgccg ccctgcgggt
     1261 ggcggcgcat cagcgcgccc accacctgct cggtgagctc ggccacctgt tcgcgcacgc
     1321 gcgccgaggc cgcaccctgg ctgccgttga ccgccaggaa agccttggtc accgccaccg
     1381 agatcttgcc cggctcgaag ccgaccaccg cgccgttgcg ccggatgacg cgatactggc
     1441 tgtagcgcgg gtcgcgcggc tgggcgtcgc cctcgatgga aagaacggcg gccgcggaac
     1501 cggccgccac ctcagtggcc gtctgcatca agctctcccc aggctgcatg tcgttcggtc
     1561 gcgacgtgcg ttctgattcg tggcgaggaa ccactacatc ttgtgtacat gcgctgattg
     1621 accactaatt ctagtgggac tggcgcgcgt gtcaagtgat agtcgcgcgc cgcgcgcgca
     1681 accggggaag actctaggct cggatcaacg cgcgaaagcg cgcccagtcg aagaacggcc
     1741 cgggatcggt cttgcgcccg ggcgcgatgt cgctgtgccc gagcaccgcc tcgatcgggt
     1801 aatgcgccat cagctcgcgg gtgagccggc tgagcgcctc gtattgcgcg acgcggaacg
     1861 gcacgaagtc ggagccttcc agctcgacgc ccaccgagaa gtcgttgcag cgctcgcgcc
     1921 cgttccagcg cgaggcgccg gcatgccagg cgcgctgcgc gcacggcacg aactggatca
     1981 gctcgccgtc gcgccgtata tagaagtggc tggacacgcg cagcccggcg atggcggggt
     2041 agaaagggtg cgcgccgggg tccaggcgat tcaggaacag gtcgatcaca cccggcccac
     2101 cgaactcgtg aggcggcagg ctgatgttgt gcaccaccac cagacggatg gcttcgccgg
     2161 cgggacgctc gtcgaaattg ggcgacgcga tctggcgggc gcgctgcagg cgcccggcgg
     2221 cgtcgaacgt ctgcgcgccg ccgcccgcgc tcatgcgtcc gctgcgcggc gccaccgccg
     2281 cgcgaccggc gcgccgggcc ggcccgcgtt cactggccgg gcgtgcccga ctccgcggtc
     2341 gtgccggcgg ccggcgcggc ctcggaggtc ctgcgcttgt actcgtgcgg cgctttccac
     2401 agggtcgctc cgcggatgcg gctcttcggg tcggtcttgc cggtgggctt caatccggcc
     2461 gaggtgccgg tcaggccgat ctcggcgagc ggcagcttgt cgctgtagcg cagccggtcg
     2521 cgcactcggg cgtagctgat caccacgtcg tcgccgctca cgctccactt gccgaaggtg
     2581 gtggagcgcc cgcgccgctt gccgggcacg ccggggaggg tgaagtcgta ctcggcgacg
     2641 atctcggccc tgccgttgtg cttcaggtgc acgtacagcc gttcgtcgtc ggggctgcgc
     2701 gcggcttccc aggtgccgag caggctcctg gagccgacgg cgtcggcggc ggccgccagc
     2761 gtgaacagga acagcaacgc gaggacgcgt gaagtcatgg cgagcggtct ccagccggcc
     2821 cggtcgctgc cgggcactta ttgacagtcg atgcccaact agtcgatgcc caacttcaat
     2881 tgatgccaag gcgctccatg cggtagcgca tggcgcggaa ggtgatgccg agcagcttgg
     2941 cggccgcggt gcggttgtag cgggtcttct ccagcgcctc gaggatcgct tccttctcga
     3001 cccggtccag gtattcctgc agcggccact tgccttccga gccctccgag gtgcgcacca
     3061 cctcttcggc ctccggcggc gtgagctgca ggtccgcggt ggtgatcttc tgcccgtcgc
     3121 acagcgccat ggcgcgctcc aggatgttct ccagctcgcg cacgttgccc gggaagtcgt
     3181 actgcgccag cgcctgcatc gcctccggct cgagctgggg cagcgacgcc ttggcctggc
     3241 cggcgagctt gtccagcacg gcgccggcga tcgccgggat gtcctcgcgc atgtcgcgca
     3301 gcgccggcat gcgcagctcg atcacgttca gccggtagta cagatcgtgg cggaacttgc
     3361 ccgactccac gcaatcggcc aggttctgat gggtggcgct gatgatgcgc acgtccaccg
     3421 cgtcctcctg ggtggagccg accttgcgcg cgcgcttctc ctggatcgcg cgcagcagct
     3481 tgacctgcat cgccagcggc aggtccgcca cctcgtccag gaacagcgtg ccgccgttgg
     3541 ccgcctggaa gaagccgtcg cggtcggcgt ccgccccggt gaaggcgccc ttgcggtagc
     3601 cgaagaactc gctctccatc aggttctcgg ggatggcgcc gcagttcacc ggcacgaacg
     3661 gccggtcgcg ccgcgcgctc ttctgatgaa tcagacgcgc ggccagctcc ttgccgctgc
     3721 cagactcgcc ggtgatgtgc accggcgctt cgctgcgcgc cagcttctcg atcaggtcgc
     3781 gcacgtgccc gatggcgggc gaagtgccga gcagaccctt ctcgcccggc gccagctcga
     3841 tcgacttggt ctcgccgacc ttgggcaggg acagcgcgga cttgaccagg gtgcgcagct
     3901 ggtcgaggct gaccggcttg gacacgtagt cgaaagcgcc ggctttgagc gccgcgaccg
     3961 cgttctcggt gttgccgtga gcggtgatca ccgccaccgg cagatccagc cccgcgcggg
     4021 tgatgttctc gacgatctcc aggccggtgc cgtccggcag ccgcatgtcg gtcaggcaca
     4081 gatcgtagtg cctgccggcg gtgatcttgg cggcggcgtc gcgcacgctc atggcgcgct
     4141 ccacgtccag ccccatgcgc agcagcgtga gctcgatcag ctcgaggatg tcgacctcgt
     4201 cgtccaccac cagcacgcag cgctggggcg cagtggcccg cccgcccgac cccacccgct
     4261 cggta
//
LOCUS       DASPGE010000079         3356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_25677, whole genome shotgun sequence.
ACCESSION   DASPGE010000079 DASPGE010000000
VERSION     DASPGE010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25677"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VD791_02435"
     CDS             complement(<1..515)
                     /locus_tag="VD791_02435"
                     /inference="COORDINATES: protein motif:HMM:NF021998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase CarF family protein"
                     /protein_id="HXV06859.1"
                     /translation="MQITPKTAEVLSQYRYGYRLVDCCAIALFAVLALWSLFRLIAAA
                     DSGPWLALATLSGWLAADLLSGLVHWAFDTWGSVHTPLLGKRFIRPFREHHWDPAAMT
                     RHDFVETSGSSCLVALPVLSATALMPLTSTAGVGAQAVLLFTALGVLLTNQCHRWAHT
                     PLARVPRLVRAA"
     gene            complement(579..1535)
                     /locus_tag="VD791_02440"
     CDS             complement(579..1535)
                     /locus_tag="VD791_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV06860.1"
                     /translation="MTTAQRKIALAAALGAFALIAPLLAPNEYVVGVLARICLYATLA
                     LGLNIVVGFAGLLDIGYVAFFGIGSYVYAFLASPQFGLHLPFLLALPIVVLATALFGI
                     LIGAPTLRLGGDYLAIVTLGFGEITYLLLLNLDRPINITNGPSGIINIDPPALLGYAM
                     SGNIQYYYLFLAVLALTLVASNRLQHSRIGWAWQAIREDELAARAMGINTTVAKLQAF
                     AIGASFAGFAGALLASWQRSVFPENFLFTESINVLAMVIIGGMGSLAGVVLGAAVIVA
                     LPEIFRELAAYRLLVFGLMLMVLMIFRPQGILVPRTAAAKKS"
     gene            complement(1639..2580)
                     /locus_tag="VD791_02445"
     CDS             complement(1639..2580)
                     /locus_tag="VD791_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV06861.1"
                     /translation="MLDALSILPQQLVFGLALGAVYGLIALGYTMVYGVLMMINFAHG
                     ELFTIGAYVGWAVLVWLINAQLGGLHPAWVLPVLLLMAMSVSGILGMTLERFAYRPLY
                     TRGATRLGPLISAVGASIFLQNAVMLTQGARMKVYMTGLLFPRSWYVQIGGVRISVLV
                     LVMISVALLLMWGLSLLVQRTTLGRCMRAVAEDREVAAVMGVNVRRVISRTFLLGSAL
                     GGAAGVLVGLYYTQIDFFMGYSAGLKAFTAAVLGGIGNIKGAMLGGLMLGVIESLAVT
                     FMNPAYRDVVAFVVLILVLVFRPTGLLGETVPQANKV"
     gene            complement(2580..3293)
                     /locus_tag="VD791_02450"
     CDS             complement(2580..3293)
                     /locus_tag="VD791_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006479711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV06862.1"
                     /translation="MLKLESVAASYGPIEVLRQVSLEVRRGEIVCLLGGNAAGKSTTL
                     KTVLGMLRPSGGSVALDDQRIDRLPTEDIVRRGIALVPESRRIFARMTVWENLAMGAY
                     SRRGAPPGDFDEDLERVYTLFPRLGERATQHGGTLSGGEQQMLAIGRSLMARPRLLLM
                     DEPSMGLAPVLVEQVFDIIQTINRQGTTILVVEQNAAVALAIAHRGYVLQDGRIVMQG
                     AAAALLDEDHIRRAYLGED"
BASE COUNT          603 a         1157 c         1154 g          442 t
ORIGIN      
        1 gccgcccgta ccaatcgcgg gacgcgggcc agcggcgtat gcgcccagcg gtggcattga
       61 ttggtgagca gcaccccgag ggcggtgaac agcaacaccg cctgcgcgcc gacgcccgcg
      121 gtcgaggtca acggcatcag cgcggtggcg ctcagcaccg gcaaagcgac caggcagctc
      181 gacccggagg tctcgacgaa gtcgtgccgg gtcatcgcgg ccggatccca gtgatgctcc
      241 cggaagggac ggatgaagcg cttgccgagc agcggcgtgt gcacgctgcc ccaggtgtcg
      301 aacgcccagt ggaccaggcc gctcagcaga tccgcggcga gccagccgga caacgtggcg
      361 agggcgagcc atggcccgga gtccgccgcc gcgatcaggc ggaacaggct ccacagggcg
      421 agcaccgcga acagcgcgat ggcgcaacag tccaccagcc ggtaaccgta ccggtactgg
      481 gaaagcactt cggcggtctt cggggtgatc tgcatcggcc ggcggctcgc agcaattcgt
      541 gtccggcggc cggacttgca agagacaagc cagcgccgtc aggatttctt cgccgccgca
      601 gtgcgcggca ccaggatgcc ctgcgggcgg aagatcatca gcaccatcag catgaggccg
      661 aacaccagca gccggtaggc ggccagctcg cggaagatct cgggcagcgc gacgatcacc
      721 gccgcgccga gcaccacgcc cgccaggctc cccatgccgc cgatgatcac catggcgagc
      781 acgttgatcg actcggtgaa caggaaattc tccgggaaca ccgagcgctg ccaggacgcg
      841 agcaacgcgc cggcgaagcc ggcgaacgag gcgccgatgg cgaacgcctg cagcttcgcc
      901 accgtggtgt tgatgcccat ggcacgcgcc gccagctcgt cctcgcggat cgcctgccag
      961 gcccagccga tgcgcgagtg ctgcaggcgg ttcgaggcga ccagcgtcag cgccagcacc
     1021 gccaggaaca ggtagtagta ctggatattg ccgctcatcg cgtaaccgag cagcgccggc
     1081 ggatcgatgt tgatgatgcc gctcgggccg ttggtgatgt tgatcgggcg gtccaggttg
     1141 agcagcagca ggtaggtgat ctcgccgaaa cccagggtga cgatggcgag gtagtctccg
     1201 cccaggcgca gggtcggcgc gccgatcagg atgccgaaca gggcggtggc cagcaccacg
     1261 atcggcagcg ccagcaggaa cggcaggtgc agaccgaact ggggcgaggc caggaatgcg
     1321 tacacgtagg agccgatgcc gaagaacgcc acgtagccga tgtcgagcag gccagcgaag
     1381 ccgaccacga tgttgaggcc gagcgccagc gtggcgtaca ggcagatgcg ggcgagcacg
     1441 ccgacgacgt actcgttcgg ggcgagcagc ggcgcgatca gcgcgaatgc gccgagcgcg
     1501 gcggcgaggg cgatcttgcg ttgcgcggtg gtcatgagtg gatggcgcct gcggcgccgc
     1561 tgttcttgca acccctcacc cccgcccctc tcccgcaagg ggagagggga gtttgttcgc
     1621 accccgagat caagctgctc ataccttgtt cgcttgcggc acggtctcgc cgagcagccc
     1681 ggtgggccgg aacaccagca ccaggatcag gaccacgaac gcgaccacgt cgcggtatgc
     1741 ggggttcatg aaggtcaccg cgaggctctc gatcacaccg agcatcaggc cgcccagcat
     1801 cgcccccttg atgttgccga tgccgcccag cacggcggcg gtgaaggcct tcagcccggc
     1861 ggagtagccc atgaagaagt cgatctgcgt gtagtacagg ccgaccagca cgccggccgc
     1921 gccgccgagc gccgatccca gcaggaaggt ccgcgagatg acgcggcgca cgttcacgcc
     1981 catcaccgcg gccacttcgc ggtcctcggc cacggcgcgc atgcagcggc cgagcgtggt
     2041 ccgctgcacc agcaggctga gcccccacat cagcagcagc gcgaccgaga tcatcaccag
     2101 caccagcacg gagatgcgca cgccgccgat ctgcacatac cagctgcgcg ggaacagcag
     2161 gccggtcatg tacaccttca tgcgcgcgcc ctgcgtgagc atcaccgcgt tctgcaggaa
     2221 gatcgacgcc ccgaccgcgc tgatcagcgg ccccaggcgc gtggcgccgc gcgtgtacag
     2281 cggccggtaa gcgaagcgct ccagcgtcat gcccagaatg ccggagacgg acatcgccat
     2341 cagcagcagc accggcagca cccaggcggg gtgcaggccg ccgagctgcg cgttgatcag
     2401 ccacacgagc accgcccagc cgacgtaggc gccgatggtg aacagctcgc cgtgggcgaa
     2461 gttgatcatc atcagcaccc cgtacaccat ggtgtagccg agggcaatca gaccgtacac
     2521 cgcgcccagc gccagcccga acacgagctg ctgcggcagg atcgacagcg cgtcgagcat
     2581 cagtcctctc ccaagtaggc gcggcggatg tggtcttcgt ccagcagcgc ggccgcggcg
     2641 ccctgcatga cgatgcggcc gtcctgcagc acgtagccgc ggtgggcgat ggcgagcgcg
     2701 accgcggcgt tctgctcgac caccaggatg gtcgtgccct ggcggttgat ggtctggatg
     2761 atgtcgaaca cctgctccac cagcaccggc gcgagtccca tcgacggctc gtccatcagc
     2821 agcaggcgcg gccgcgccat cagcgagcgc ccgatggcga gcatctgctg ctcgccgccg
     2881 gagagcgtgc cgccgtgctg cgtggcgcgc tctcccaggc gcggaaacag ggtatagacg
     2941 cgctcgaggt cctcgtcgaa gtcccccggc ggcgcgccgc ggcggctgta cgcgcccatc
     3001 gccaggttct cccacaccgt catgcgcgcg aagatgcggc gcgactctgg caccagggcg
     3061 atgccgcggc gcacgatgtc ctcggtcggc aggcggtcga tgcgctggtc gtcgagcgct
     3121 acgctgccgc cgctcgggcg cagcatgccc agcacggtct tgagcgtggt ggacttgccg
     3181 gcggcattgc cgcccagcag gcagacgatc tcgccgcgcc gcacttccag gctcacctgg
     3241 cgcagcacct cgatcgggcc gtagctcgca gccacgctct ccaacttaag catggcgtcg
     3301 cccgaggtag gcttccagca cggcggggtt gtggcgcacc tcctggaagc tgccgt
//
LOCUS       DASPGE010000080        14398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1197256, whole genome shotgun sequence.
ACCESSION   DASPGE010000080 DASPGE010000000
VERSION     DASPGE010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14398
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197256"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="VD791_02455"
     CDS             complement(<1..1155)
                     /locus_tag="VD791_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HXV06863.1"
                     /translation="MLDVSTYRERRQRLTRQMGRGVAVISTAPERLRNRDAHYPFRFD
                     SYFHYLTGFPEPESVAVIVAGDQAKSLIFCRDKDLEREIWDGFRHGPEAARQVFGFDE
                     GHSFSKLDEMMPGLLADQPAIFCDVGENSEWDARLIQWLNAVRAQVRTGVSAPGEIRD
                     VRKLLDEMRLIKDAAELDVMRRAAAISAAAHRRAMQATRPGRCEYQIEAELLHEFRAH
                     GAQAPAYTPIVAGGANACVLHYTTNDAPLRDGDLVLIDAGCELAGYAADITRTFPVGA
                     RFSGAQRDVCQVVLAAQAAAINRVRPGCAWNEPHDAAVRALAQGMIDLKLLAGGLDEV
                     LEKESYKRFYMHRTGHWLGLDVHDAGDYKEAGEWKPLQPGMTLTVEPGCYI"
     gene            complement(1243..1875)
                     /locus_tag="VD791_02460"
     CDS             complement(1243..1875)
                     /locus_tag="VD791_02460"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HXV06864.1"
                     /translation="MLYLPGSFAENDRATLQAFIAAHGFATLISPDAKDPQVTHLPLL
                     LDPERSTLGALLGHVARANPHWRLLESRTEVLAIFHGPHAYVSPSWYLHHPSVPTWNY
                     AVVHVQGRARVTHDARELERIVRRLVDRYESARPQPWSMQLPADYQAQMLAGIVGFEI
                     EIVRISGKFKLSQNRPVADRPRVAEVLSGGGPHERELAQLMQRYYGIGKA"
     gene            complement(1890..2579)
                     /locus_tag="VD791_02465"
     CDS             complement(1890..2579)
                     /locus_tag="VD791_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXV06865.1"
                     /translation="MHVMILAAGRGERMRPLTDATPKPLLPLAGKPLIVWHLERLAAA
                     GLHEVVINHAWLGERIEALLGDGTRYGVRIRYSAEGSALGTAGGIARALPLLGAGTFL
                     VINGDVYTHIDFVALAARASDIGPQAALAHLVLVSNPEHHPDGDFALDGGRVHSAGAR
                     RLTFSGVGLYAPQLFAGIAAGTRAHLAPLLHKAMAEGRVTGEHFTGCWTDVGTPERLA
                     QLDARLRTRPI"
     gene            complement(2596..3594)
                     /locus_tag="VD791_02470"
     CDS             complement(2596..3594)
                     /locus_tag="VD791_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HXV06866.1"
                     /translation="MSRNLQFEQWLAQHYAGRRFAFAPASEDASFRRYLRVTPETGPT
                     VILMDAPPEREDCRPYVEVARLFGAAGVHVPEVLAADLENGYLLLSDLGTRTYLQALQ
                     DKRSASALYDDATDALVRIQLASRPGVLPPYDRTLLLREMRLFPDWYIARHLGVTLTP
                     SLNQALDLVFERILANNLAQPCVYVHRDYHSRNLMVTTPNPGILDFQDAVYGPITYDL
                     VSLLKDAYVSWEEATILDWAVRYWEKARKAGLPLASDFAAFYRDFEWMGAQRHIKVLG
                     IFARLFHRDGKNGYLKDMPLVLRYLRQACERYGELHPLLRLLDQLEHRQPSTGYTF"
     gene            3658..5934
                     /locus_tag="VD791_02475"
     CDS             3658..5934
                     /locus_tag="VD791_02475"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HXV06867.1"
                     /translation="MAETGRAARAALAFVVGLWTLPAALAQTSSDQGASLDPDRGGLG
                     LRLERELMAPTAPGPGEQLPLFLEADRIEGVQDRYVEASGNVVVRRRGQTLFADRLRY
                     SIPDNAVSASGNVESNRLGDVIAGDEAYYDLDTESGYIQKPRFNFSQIRGRGQASRLS
                     VRDRDRYRAERATYTNCAVGDDDWYLRVGQLDLDRLRDVGTARNATLVFKGVPILYTP
                     WIDFPLSSRRKSGFLAPVVGTSNNSGFEAMLPFYWNIAPNYDYTIAPRVLARRGLQIN
                     NEVRYLRPSLNGQLRADVLPDDQEKDGDTRWGYSFQHSQLLTQRLRGWVNYQGVSDDT
                     YFVDLSNKIAATSQTNLPRELGLSYNGDWWNLMARSQRFQTLQDPLAPVEEPYARVPQ
                     LVLNAAQQNLRGFDLQFFGEVVDFDHPDKVSALRQVYYPAAVFPVRGSFWYVAPKFGV
                     NHTIYAYQEGSRSDDTRTLPIFSVDSGMAFDRATAFFGRSYRQTLEPRLYYVRIPFER
                     QDDLPLFDTSQADFNLAQIFTENQFTGWDRINDADQITAAVTTRLLEPTSGAEWVRAT
                     LGQRYYFKEQEVTLNNQVRSSNRSDLLAGVAGNLTRNWSASVGLQYNVDGGEFEKFNS
                     VLRYRPQPGKVVNLGYRFTREVLEQVDLAAQWPLSRRWSGLARWNYALEGSALLQGLV
                     GLEYSANCWAARFVAHSFVTATDERSNAFFAQLELSGLSRLGISPLELLRQSIVGYQT
                     TGGQSAAPEVYYPGMEYR"
     gene            6069..7304
                     /locus_tag="VD791_02480"
     CDS             6069..7304
                     /locus_tag="VD791_02480"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF020872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXV06868.1"
                     /translation="MAQRVELLDRIVAVVNADVITRLELDRENSIAQEALRRQGTPLP
                     QRDVLEKQLLERLITKNVLMQYARQTGLRVTDPEVEAALERIAAENKMKSAELRQAVE
                     QSGMPFSRFREDLRGELLISKLREREVEAKIAVTDTEIQNFLRTQEAQPERVDEYNLS
                     HILITVPEQASPEELKLRRGRAENALTQIRQGGDFRQAAASYSDAPDALQGGDMGWRP
                     TGRLPTIFLQALKDMKVGQVSDIVRSPAGFHILKLNDKRGNDTPILVNQTRARHILVR
                     LNEVVSENDARNRLGELKDRIEHEADFAELARLHSDDTTAARGGDLGWVSPGDTVPEF
                     ERAMAALQPGQVSQPFKTAFGWHIVQVLERREQDMSKDRQRIQARQAIRSRKSDEQWQ
                     EWVRQQRDKAYVEYKLEEK"
     gene            7301..8101
                     /gene="rsmA"
                     /locus_tag="VD791_02485"
     CDS             7301..8101
                     /gene="rsmA"
                     /locus_tag="VD791_02485"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HXV06869.1"
                     /translation="MKYEARGARHVLRKRFGQHFLVDPTVVASILEAISPQAGDHVVE
                     IGPGLGALTEPLLQRLPSLDAVEVDRDLAAALAARFGPERLRMHVADALQFDFCALGA
                     PLRVVGNLPYNISTPLLFHLAGCIACLLDCHFMLQREVVQRIAAKPGGKDYGRLSVML
                     QYRFEARELLRVAAGAFRPVPAVESTLVRLTPRRPLPWPARDEGLLAALVAKAFMQRR
                     KTLRNALRDRVEESDFDAAQIDPGQRPEALSVAQFVRLADVVTAKRQA"
     gene            complement(8102..8491)
                     /gene="gloA"
                     /locus_tag="VD791_02490"
     CDS             complement(8102..8491)
                     /gene="gloA"
                     /locus_tag="VD791_02490"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HXV06870.1"
                     /translation="MRILHTMLRVGDLERSLAFYTQVLGMQLLRRRDYPDGKFTLAFV
                     GYGDEEHDAVLELTYNWGVDKYQLGNAYGHIAVEVDDARRACEEVKKRGGKVTREAGP
                     MKHGSTVIAFVEDPDGYKIEFIQRKAA"
     gene            complement(8511..8696)
                     /locus_tag="VD791_02495"
     CDS             complement(8511..8696)
                     /locus_tag="VD791_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HXV06871.1"
                     /translation="MSAQAAPYRTYMCLICGFIYDEAAGLPEEGIAPGTRWEDVPVNW
                     VCPECGARKEDFELVEV"
     gene            8775..9602
                     /locus_tag="VD791_02500"
     CDS             8775..9602
                     /locus_tag="VD791_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HXV06872.1"
                     /translation="MPESPPIVLAFAATDPSAGAGLQADILTLSSMGCHPLSVVTAIT
                     IQDTAGVEDVLALDAEWVADQARCVLEDMPVSVFKVGLLGSVEIITAIAEIVSDYPEV
                     PLVLDPVLASGRGDELATDEIIAAMRELLVPQTTVLTPNSLEARRLVQEDGDESDYPA
                     LDECARRLLTLGTEFVLITGTHENTPEVVNTLYSREGVVRSDRWQRLPGAFHGSGCTL
                     AAAIAATLANGLDVAEAVRDAQEYTWQALGAAYRPGMGQHIPDRLFWARDEEQEGQA"
     gene            9614..10258
                     /gene="thiE"
                     /locus_tag="VD791_02505"
     CDS             9614..10258
                     /gene="thiE"
                     /locus_tag="VD791_02505"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HXV06873.1"
                     /translation="MDAPHLHGLYAVTPDTLDDAWLAERVEQALRGGARAVQYRSKHA
                     DAQLRRRQAQRLLQACRAHGALFVVNDDVDLALELEADGVHLGRDDLSVAAARARLRR
                     GALIGASCYDDLARAERAAQAGADYLAFGSLFPSAVKPGAVRASLELLRQARGRWRLT
                     LVAIGGIEPDNAARVIQAGADAVAVITALFSVPDTLAAARALSRLFQQPTMPAL"
     gene            10255..11541
                     /gene="hemL"
                     /locus_tag="VD791_02510"
     CDS             10255..11541
                     /gene="hemL"
                     /locus_tag="VD791_02510"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HXV06874.1"
                     /translation="MKTRNETLFELSQQVIPGGVNSPVRAFRAVGGTPVFFQRAQGAH
                     FWDADGKRYIDYVGSWGPLVLGHAHPDVVDAVRRAAQDGLSFGAPTEAEYEMAALLTR
                     LLPSLELVRLVSSGTEATMTAIRLARGFTGRNRIVKFEGCYHGHADALLVKAGSGALT
                     LGVPSSAGVPPEVTAHTLVLAFNDLSGLERAFGEQGRDIAGVIVEPVAGNMNLVQGSE
                     SFLNGLRELCTRHGAVLIFDEVMTGFRVGLQGAQGLYGIRPDLTTLGKIVGGGMPLGA
                     FGGRRDIMQCVAPLGPVYQAGTLSGNPLAVAAGLATLKAVQAPGFYARLEATAAGLTE
                     GLAHAAREAGVSFSAQSVGGMFGLYFSAQPPRTFAEVMQSDREAFNRFFHAMLQQGVY
                     FAPSAFEAGFVSCAHGAEDIEATVAAARRAFAGLGR"
     gene            complement(11583..12206)
                     /locus_tag="VD791_02515"
     CDS             complement(11583..12206)
                     /locus_tag="VD791_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV06875.1"
                     /translation="MKHLAAAALAAALAASPAAAQPDAARAGDAARGQTIAAGVCAAC
                     HGGDGNSPIGANPNLAGQHAEYLVKQLQNYKSGERNNPIMTAIATTLSDADARDVAAY
                     YAAQGSRPGFARDARLASAGRALYRGGNSASGVAACSGCHSPNGAGIPVQYPRLKGQH
                     GEYTAAQLRAFRSGERANDPASMMRGVAARMSDAEIAAVAEYISGLK"
     gene            12348..12989
                     /gene="yihA"
                     /locus_tag="VD791_02520"
     CDS             12348..12989
                     /gene="yihA"
                     /locus_tag="VD791_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HXV06876.1"
                     /translation="MLEKAQFRIGAADWSQLPPDHGREVAFAGRSNAGKSSAINAITN
                     RRRLAFVSKTPGRTQQVNFYALGAGRHLVDLPGYGYARVAPELQQRWARMLSAYLQNR
                     AALRGLVLIMDVRRPLTPLDRALLDWFAPTAKPVLALLTKADKLSRQQATRQLDAVCA
                     MFERSYPGARAILFSAVSRLGVEAAQGFVAGLLGGQGEAPAEYNSGAQRKAPG"
     gene            13142..14146
                     /gene="hemB"
                     /locus_tag="VD791_02525"
     CDS             13142..14146
                     /gene="hemB"
                     /locus_tag="VD791_02525"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HXV06877.1"
                     /translation="MPVLGKYPQRRMRRNRHDGFSRRLVREHTLTPDDLIYPVFVIEG
                     EGNAQPVESMPGVSRLTLDRLLPMAERCLRLGVPALALFPAIEPRLKTPDGREAINPK
                     GLIPRVVAGLKKRFPELGVMTDVALDPFTSHGQDGVIDAAGYILNDETIAILQKQVLT
                     QAEAGVDIVAPSDMMDGRVGALRAALDAQGFIHTRIMAYAAKYASAFYGPFRDAVGSA
                     ANLGQGDKLTYQMDPANGDEALWEVGLDLEEGADMVMVKPGLPYLDIVRRVKDAYRAP
                     TFVYQVSGEYAMLKAAFQNGWLDERKCVLEALLAFKRAGADGILTYFALDAAEWLRNR
                     "
BASE COUNT         2261 a         4843 c         5013 g         2281 t
ORIGIN      
        1 gatgtagcac cccggctcca cggtgagcgt catgcccggc tgcagcggct tccactcgcc
       61 cgcctccttg tagtcgccgg cgtcgtgcac gtcgagcccc agccagtgcc cggtgcggtg
      121 catgtagaag cgcttgtagg attccttctc gagcacttcg tcgaggccgc cggcgagcag
      181 cttcaggtcg atcatgccct gcgcgagcgc gcgcaccgcc gcgtcgtgcg gctcgttcca
      241 ggcgcagccg ggccgcaccc tgttgatcgc cgcggcctgc gccgcgagca ccacctggca
      301 gacgtcgcgc tgcgcgccgg agaagcgcgc gcccacgggg aaggtgcggg tgatgtcggc
      361 ggcgtagccg gccagctcgc agcccgcgtc gatcagcacc aggtcgccgt cgcgcagcgg
      421 cgcgtcgttg gtcgtgtagt gcagcacgca ggcgttggcg ccgccggcga cgatcggtgt
      481 gtaggccggc gcctgtgcac cgtgcgcgcg gaactcgtgc agcagctcgg cctcgatctg
      541 atactcgcag cgtccgggcc gggtggcctg catggcgcgg cggtgcgcgg ccgccgagat
      601 cgccgcggcg cgtcgcatga cgtcgagctc ggccgcgtcc ttgatcaggc gcatctcgtc
      661 gagcagcttg cgcacgtcgc ggatctcgcc gggtgccgac acgccggtgc gcacctgtgc
      721 acgcaccgcg ttgagccact ggatcaggcg cgcgtcccac tcgctgttct cgccgacgtc
      781 gcagaagatc gccggctggt cggcgagtaa tccgggcatc atctcgtcga gcttggagaa
      841 cgagtgccct tcgtcgaagc cgaacacctg ccgcgccgct tccggcccat ggcggaaacc
      901 gtcccagatt tcgcgctcca gatccttgtc gcggcagaag atcaagctct tcgcctggtc
      961 gccggccacg atcaccgcga ccgactccgg ctcggggaag ccggtgagat agtggaagta
     1021 gctgtcgaac cggaacgggt aatgggcgtc gcggttgcgc agccgctccg gggcggtgga
     1081 gatcaccgcc acgccccgac ccatctgtcg ggtgaggcgc tggcggcgct cgcggtaagt
     1141 ggatacgtcg agcatgtcgt gattataaag gtgcgatcgg tgaaacgtga tcgacaaggg
     1201 gtgatcgaaa aggcgtgatc gaacagcgca ggcgccaacc cgctacgctt tgccgattcc
     1261 gtagtaacgc tgcatcagct gcgccagctc ccgctcatgc ggcccgcctc cggagaggac
     1321 ttcggccacg cgcggacggt ccgccaccgg ccggttctgg ctcagcttga acttgccgct
     1381 gatgcgcacg atctcgatct cgaagccgac gatgccggcc agcatctgcg cctggtagtc
     1441 ggcgggaagc tgcatgctcc agggctgggg acgcgcgctt tcgtacctat ccaccagccg
     1501 ccgcacgata cgctccagct cccgggcatc gtgggtgacg cgcgcgcggc cctgcacatg
     1561 caccaccgca tagttccagg tcggcacgct cggatgatgc aggtaccagc tcggcgaaac
     1621 gtaggcgtgc gggccatgga agatcgccag cacttcggtc cgggactcga ggaggcgcca
     1681 gtgcgggttg gcgcgggcga cgtgccccag gagcgcaccc agcgtgctgc gctccgggtc
     1741 cagcagcagc ggcaggtgcg tgacctgggg atccttcgcg tcgggcgaga tcagcgtggc
     1801 gaagccgtgc gccgcgatga acgcctgcag ggtggcgcga tcgttctcgg cgaaactgcc
     1861 ggggaggtac aacatcgtgc ggcttcctct caaatcgggc gcgtgcgcag gcgcgcgtcg
     1921 agctgcgcca ggcgctcggg cgtgcccacg tcggtccagc agccggtgaa gtgctcgccc
     1981 gtgacgcgac cctcggccat cgccttgtgc agcagcggcg cgaggtgtgc tcgcgtgccg
     2041 gccgcgatgc cggcgaacag ctgcggcgcg tacagcccca cgcccgagaa ggtcaggcgg
     2101 cgcgcgcccg cgctgtgcac gcgcccgccg tcgagcgcga aatcgccgtc gggatgatgc
     2161 tccggattgg acaccagcac caggtgcgcg agcgccgctt gcgggccgat gtcggacgcg
     2221 cgcgcggcca gggccacgaa gtcgatgtgc gtgtacacat cgccgttgat caccaggaac
     2281 gtgccggcac cgagcagcgg cagggcgcgt gcaatgccgc ccgccgttcc cagcgcgctg
     2341 ccctccgccg agtagcggat gcgcacgccg tagcgggtgc cgtctccgag cagcgcttcg
     2401 atccgctctc cgagccacgc gtggttgatc accacctcgt gcaagcccgc agcggccagc
     2461 cgctcgagat gccagacgat cagcggcttg ccggccaggg gcagcagcgg cttgggtgtg
     2521 gcgtcggtca gcggccgcat gcgctcgccg cgaccggcgg ccaggatcat cacgtgcatg
     2581 cgacgtggct ccacgtcaga aggtgtaacc cgtgctcggt tgcctgtgct cgagctggtc
     2641 gagcaggcgc agcaggggat gcagctcgcc atagcgctcg caggcctggc gcaggtagcg
     2701 caggaccagc ggcatgtcct tcaggtagcc gttcttgccg tcgcggtgga acaggcgcgc
     2761 gaagatgccc agcaccttga tgtggcgctg cgcgcccatc cattcgaagt cgcggtagaa
     2821 agcggcgaaa tccgaggcca aaggcaggcc ggccttgcgc gccttctccc agtagcgcac
     2881 cgcccagtcg agaatcgtcg cctcctccca gctgacgtat gcgtccttca gcaacgacac
     2941 caggtcgtag gtgatcggcc cgtacaccgc gtcctggaaa tccaggattc cgggattggg
     3001 tgtggtcacc atcaggttac gcgagtggta gtcgcggtgc acgtacacgc agggctgcgc
     3061 cagattgttg gcgaggatgc gctcgaacac caggtccagc gcctggttca gggacggggt
     3121 caacgtcacc ccgaggtgtc tggctatgta ccagtcggga aacagccgca tctcgcgcag
     3181 cagcagcgtg cggtcgtagg gcggcagcac gccggggcgg ctcgccagct ggatgcgcac
     3241 cagggcatcg gtggcgtcgt cgtacagtgc gctcgccgaa cgcttgtctt gcaacgcctg
     3301 caggtaggtt cgggtgccga ggtccgacag caggaggtag ccgttctcca gatccgcggc
     3361 gagcacctcc ggcacgtgca cgccggcggc gccgaacagg cgcgcgacct cgacgtaggg
     3421 ccggcaatcc tcgcgctccg ggggcgcatc catgaggatg acggtcgggc ccgtttcggg
     3481 ggtgacccgc agatagcgcc ggaagctggc gtcctcggag gcgggggcga aggcgaagcg
     3541 gcgccccgca tagtgctgcg caagccactg ctcgaactgg agattgcggc tcaaatcgcg
     3601 tgtttgcctg gagcttgctt ttgtgcgatt ttatcggcaa atcacaacct ggcctaaatg
     3661 gcagaaaccg gtcgcgccgc gcgggcggcg ctggcgttcg tcgtcggcct gtggacgctt
     3721 cccgcagcgc tggcccagac ctcctccgac cagggcgcat ccctcgaccc cgaccgcggc
     3781 ggcctcggac tcaggctgga acgcgagttg atggcgccga ccgcgcccgg ccccggggag
     3841 cagttgccgc tgttcctcga ggccgaccgc atcgagggcg ttcaggaccg ctacgtggag
     3901 gcgagtggca acgtggtggt gcggcgtcgc gggcagacgc tgttcgcgga ccggttgcgc
     3961 tattcgattc cggacaacgc cgtcagcgcc agcgggaacg tcgagtcgaa ccgcctgggc
     4021 gacgtgatcg cgggggacga ggcctactac gatctggaca ccgaatcggg ttacatccag
     4081 aagccgcgct tcaatttctc ccagatccgc ggccgcggcc aggccagccg tctcagcgta
     4141 cgcgaccgtg atcgctaccg cgcagagcgc gccacgtaca ccaactgcgc ggtgggcgat
     4201 gacgactggt atctgcgagt ggggcaactg gacctggatc gcctgcgcga cgtcgggacg
     4261 gcacgcaacg ccacgctggt gttcaagggt gtgccgatcc tgtacacacc ctggatcgac
     4321 ttcccgctga gcagccgccg caagagcggc tttctggcgc cggtggtcgg caccagcaac
     4381 aacagcggct tcgaagccat gctcccgttc tactggaaca tcgcgccgaa ctacgactac
     4441 accatcgcac cgcgcgtact cgccaggcgc ggactgcaga tcaacaacga ggtgcgctat
     4501 ctgcggccga gcttgaacgg gcagctgcgg gcggacgtgc tgcccgacga tcaggagaaa
     4561 gacggcgaca cgcgctgggg ttacagcttc cagcactcgc agctgctgac ccagcgcctg
     4621 cgcggctggg tgaactacca gggcgtgtcc gacgacacct acttcgtcga tctgtccaac
     4681 aagatcgcgg ccacttcgca gaccaacctg ccgcgcgagc tcgggctcag ctacaacggc
     4741 gattggtgga acctgatggc tcgttcgcaa cgcttccaga ccctgcagga tccgctggcg
     4801 ccggtggagg agccttacgc ccgcgtcccg cagctggtgc tgaacgccgc gcagcagaac
     4861 ctgcgcgggt tcgacctgca gttcttcggc gaggtggtcg atttcgacca tccggacaag
     4921 gtgagcgcgc tgcggcaggt gtattacccg gcggccgtgt ttccggtgcg cggttcgttc
     4981 tggtacgtgg cgcccaagtt tggcgtcaac catacgatct acgcctacca ggagggttcg
     5041 cgcagcgacg acacccgcac cctgccgata ttcagcgtgg acagcggcat ggctttcgac
     5101 cgcgcgactg cgttcttcgg gcgcagctat cgtcagacgc tggagccgcg gctatactac
     5161 gtgcgcatcc cgttcgagcg ccaggacgat ctgcccctgt tcgacacctc gcaagccgac
     5221 ttcaacctgg cgcagatctt caccgagaat cagttcaccg gctgggaccg catcaacgat
     5281 gccgaccaga tcaccgccgc ggtgaccacc cggctgctcg aacccacctc gggcgccgag
     5341 tgggtgcgcg ccacgctcgg ccagcgctac tacttcaagg agcaggaggt gaccctcaac
     5401 aaccaggtgc gcagttcgaa ccgctccgat ctgctggcag gcgttgccgg caacctgacg
     5461 cgcaactggt cggcgagcgt gggcctgcaa tacaacgtgg acggcgggga gttcgagaag
     5521 ttcaactccg tgctgcgcta ccggccgcag cccggcaagg tcgttaacct gggctaccgg
     5581 ttcacgcgcg aggtgctgga gcaggtggat ctcgctgccc agtggccgct gtcgcggcgc
     5641 tggtcgggcc tggcgcgctg gaactatgcc ctcgagggca gtgcgctgct gcaggggctg
     5701 gtgggcctcg agtacagcgc gaactgctgg gcggcgcgct tcgtcgcgca cagcttcgtc
     5761 accgccaccg acgagcgctc caacgccttc ttcgctcagc tcgagctgag cggcctgtcg
     5821 cgcctgggca tcagcccgct cgagctgctg cggcagagca tcgtcggcta ccagaccacc
     5881 ggcggccagt cggccgcgcc ggaggtctac tatcccggca tggagtaccg atgaatcgac
     5941 cggcagccgt ccctctcccg cagccggcgc tgacgccgcg gtgcagtgtt gggcgcgccc
     6001 cgacgcgcgc cggcgcgttg ctgtctgttc tcgcggccct ggcgccggcg gccccagcgc
     6061 cggtagccat ggcgcagcgc gtcgagctgc tcgaccgcat cgtggcggtg gtcaacgccg
     6121 atgtgatcac ccggctggag ctcgaccggg agaacagcat cgcgcaggaa gcgctgcgcc
     6181 gtcagggcac acccctgccc cagcgcgacg tgctggagaa gcagctcctc gagcggttga
     6241 tcacgaagaa cgtgctgatg cagtacgcac ggcagaccgg gttgcgcgtg accgaccccg
     6301 aggtggaggc ggccctcgag cgcatcgccg cagagaacaa gatgaaaagc gccgagctgc
     6361 gccaggcggt ggagcagagc ggcatgccgt tcagccgctt ccgcgaggac ctgcgcggcg
     6421 agctgctgat ctccaagcta cgcgagcgcg aggtcgaagc caagatcgcg gtgaccgaca
     6481 cggagatcca gaacttcctg cgcacccagg aggcgcagcc ggaacgcgtg gacgaataca
     6541 acctgtcgca catcctgatc accgtgccgg agcaggcgag ccctgaggag ctcaagctgc
     6601 gccgtggccg cgccgagaat gcgctcacgc agatccgaca gggcggcgac tttcggcagg
     6661 ctgcggcgag ctattccgac gcgcccgatg ctctgcaggg tggcgacatg gggtggcgcc
     6721 cgacgggccg actgccgacg atcttcctgc aggctctcaa ggacatgaag gtgggtcagg
     6781 tgtccgacat cgtgcgcagc ccggccggtt tccacatcct gaagctcaac gacaagcgcg
     6841 gcaacgacac cccgatcctg gtcaaccaga cgcgcgcgcg ccatatcctg gtgcggttga
     6901 acgaggtggt atccgagaac gacgcgcgca accgcctggg cgagctcaag gaccgcatcg
     6961 agcacgaagc cgacttcgcc gagctcgcgc ggctgcattc ggacgacacc accgcggccc
     7021 gcggcggcga cctgggctgg gtgtcgccgg gagacacggt gcccgagttc gagcgcgcca
     7081 tggcggccct gcaaccgggg caggtgagcc agccgttcaa gaccgccttc ggctggcaca
     7141 tcgtgcaggt gctggagcgc cgcgagcagg acatgtccaa agaccgccag cgtatccagg
     7201 cgcgccaggc gatccgttcg cgcaagtcgg acgagcagtg gcaggaatgg gtgcgccagc
     7261 agcgcgacaa ggcgtacgtg gaatacaagc tcgaggagaa gtgaagtacg aggcacgagg
     7321 tgcgaggcac gtcctgcgca agcgcttcgg gcagcatttt ctggtcgacc ctacggtggt
     7381 cgcgagcatc ctcgaggcga tctcgccgca ggcgggcgac cacgtggtgg agatcggccc
     7441 cggcctgggc gcactcaccg agccgctgct gcagcgcctg cccagcctgg acgcggtgga
     7501 ggtcgaccgc gacctcgcgg cagcgctggc cgcgcgcttc ggcccggagc ggctgcgcat
     7561 gcacgtcgcc gatgcgctgc agttcgactt ctgcgccctc ggcgcgcctc tacgcgtggt
     7621 gggcaacctg ccctacaaca tctcgacgcc gctgctgttc cacctcgccg gctgcatcgc
     7681 ctgcctgctg gactgccact tcatgctcca gcgcgaagtg gtacagcgca tcgccgccaa
     7741 gccgggcggc aaggactacg ggcggctgtc ggtaatgctg cagtatcgct tcgaggcgcg
     7801 cgagctgctg cgtgtcgcgg ccggcgcgtt ccgcccggtg ccggcggtgg agtcgacgct
     7861 ggtgcggttg acgccgcgcc gcccgctgcc ctggccggcc cgcgacgagg gcctgctcgc
     7921 tgcgctggtg gcgaaggcgt tcatgcagcg gcgcaagacg ctgcgcaacg cgctgcgcga
     7981 tcgcgtcgag gaaagcgatt tcgacgccgc gcagatcgac ccgggccagc ggcccgaggc
     8041 gctgtcggtt gcgcagttcg tgcggctcgc cgacgtggtg acggcgaaac ggcaggcttg
     8101 actaagccgc cttgcgctgg atgaactcga tcttgtagcc gtccgggtct tcgacgaagg
     8161 cgatcaccgt gctgccatgc ttcatcggcc cggcctcgcg cgtcaccttg ccgccgcgtt
     8221 tcttcacttc ctcgcaggcg cgccgggcgt cgtccacctc gacggcgatg tgaccgtagg
     8281 cgttacccag ctggtacttg tccacgcccc agttgtaggt gagctccagc accgcgtcgt
     8341 gctcctcgtc gccgtagccg acgaaggcga gcgtgaactt gccgtcgggg taatccctgc
     8401 gccgcagcag ctgcatgccg agcacctggg tgtagaacgc cagcgagcgc tcgagatcgc
     8461 cgacgcgcag catggtgtgc aggatacgca tgatcgagaa cctccgtgtc tcagacttcg
     8521 accagctcga agtcttcctt gcgcgcgccg cactccgggc acacccagtt caccggcacg
     8581 tcctcccagc gcgttcctgg cgcgatgcct tcttcgggca ggccggctgc ttcgtcgtag
     8641 atgaagccgc agatgagaca catgtatgtg cggtacggcg ctgcttgtgc gctcatcgtt
     8701 ctgcagttgt ctttgggggt gactctttta gagtagaatc gaattctagc aaatccctcc
     8761 tcgcagccta attcatgccc gaatcgcccc cgatcgtcct cgcgttcgcc gccaccgatc
     8821 cgagcgcagg cgcagggctg caggcggaca ttctcaccct gtcgagcatg ggttgccacc
     8881 cgctgtcggt ggtcaccgcg atcaccatcc aggacaccgc aggcgtcgag gacgtgctgg
     8941 cgctggacgc cgagtgggtg gcggaccagg cgcgctgcgt gctggaggac atgccggtaa
     9001 gcgtgttcaa ggtcggattg ctcggcagcg tcgagatcat caccgccatc gccgagatcg
     9061 tgtccgacta tccggaggtg ccgctggtgc tcgacccggt gctggcatcg ggccggggcg
     9121 acgagctggc gaccgacgag atcatcgccg ccatgcgcga gctgctggtc ccgcagacca
     9181 ccgtgctcac gcccaacagc ctggaggcgc ggcgcctggt acaggaggac ggggacgaga
     9241 gcgattaccc tgcgctggac gagtgcgcgc gccgtctgct cacactgggc accgagttcg
     9301 tcctgatcac cggcacgcac gaaaatacgc ccgaagtggt caacacgctc tattcgcgcg
     9361 aaggcgtggt gcgcagcgac cgctggcagc gcctgcccgg tgccttccac ggatcgggtt
     9421 gcacgctcgc cgcggccatc gccgcgacgc tcgccaacgg actcgacgtc gccgaggccg
     9481 tgcgcgacgc gcaggaatac acctggcaag cgctgggcgc ggcttatcgt ccggggatgg
     9541 gccagcacat cccggaccgc ctgttctggg cgcgcgacga ggagcaggaa ggccaggcat
     9601 aggcccgcgc cggatggatg cgccgcatct gcatgggctg tacgccgtga cgccggacac
     9661 gctcgacgac gcctggctcg ccgagcgtgt cgagcaggcc ctgcgcggcg gggcgcgcgc
     9721 ggttcagtac cgcagcaagc acgccgacgc gcaactgcga cgacgccagg cgcagcggct
     9781 gttgcaggcg tgccgtgcgc atggggcgct gttcgtcgtg aacgacgatg tggatctcgc
     9841 gctcgagctc gaggcggacg gggtgcacct gggccgcgac gacctgtccg tggcggcggc
     9901 gcgcgcgcgc ctgcggcgtg gcgcgctgat cggcgcttcc tgctacgacg acctggcccg
     9961 cgccgagcgc gcggcgcagg ccggcgcgga ctacctcgcg ttcggaagct tgttcccgtc
    10021 ggcggtgaag ccgggcgcgg tgcgcgcgtc gctcgagctc ctacgccagg cgcgggggcg
    10081 ctggcgcctg accctggtgg cgatcggcgg catcgagccg gacaacgcgg cacgcgtgat
    10141 ccaggccggg gcggacgcgg tggcggtgat caccgcgctg ttcagcgtgc ccgacacgct
    10201 cgccgccgcg cgcgcgttgt cgcgactgtt ccagcaaccg accatgcccg cgctatgaag
    10261 acccgaaacg aaacgctgtt cgagctttcc cagcaggtca ttccaggcgg cgtgaactcg
    10321 ccggtgcgcg ccttccgcgc ggtgggcggg accccggtgt tcttccagcg cgcgcagggt
    10381 gcgcacttct gggatgccga cggcaagcgc tacatcgact atgtcggctc ctgggggccg
    10441 ctggtgctgg ggcacgcgca cccggacgtg gtggatgcgg tgcggcgcgc ggcgcaggac
    10501 ggcctgagct tcggcgcgcc cactgaggcc gaatacgaga tggcggcgct gctcactcgc
    10561 ctgctgccgt cgctcgagct ggtgcggctg gtgagctccg gcaccgaggc caccatgacc
    10621 gcgatccggc tcgcgcgcgg tttcaccggc cgcaatcgca tcgtcaagtt cgagggctgc
    10681 tatcacgggc acgccgacgc gctgctggtg aaggccgggt cgggcgcgct gacgctgggc
    10741 gttcccagct cggcgggtgt gcccccggag gtgaccgcgc acaccctggt gctcgcgttc
    10801 aacgacctct cggggctgga gcgcgccttc ggcgagcagg gccgggacat cgccggcgtc
    10861 atcgtcgagc cggtggccgg caacatgaat ctggtgcagg gatcggagag cttcctcaac
    10921 ggcttgcgcg agctgtgcac gcgccacggt gcggtgctga tcttcgacga ggtgatgacc
    10981 gggtttcgcg tcggcctgca gggcgcgcag ggcctgtacg gcatccgccc ggatctgacc
    11041 acgctcggca agatcgtcgg cggcggcatg ccgctcggcg ccttcggcgg caggcgcgac
    11101 atcatgcagt gcgtcgctcc gctggggccg gtgtatcagg ccggaacgct gtccggcaat
    11161 ccactggcgg tggccgcggg tctggcgacg ctgaaggcgg tgcaggcccc cgggttctac
    11221 gcgcgcctgg aagcgaccgc ggccggcctg accgagggcc tggcgcacgc cgcacgcgag
    11281 gccggggtga gcttcagcgc gcagtcggtg gggggcatgt tcgggctgta tttctccgcg
    11341 cagccgccgc gcaccttcgc ggaagtgatg caaagcgacc gggaggcgtt caaccgcttc
    11401 ttccacgcca tgctgcagca gggcgtgtat ttcgcgccgt ctgcgttcga agcgggtttc
    11461 gtgtcctgcg cgcacggcgc cgaggacatc gaggccacgg tcgccgcagc gcggcgcgcg
    11521 ttcgccggac tgggccgctg aaggcgccag ccgcccgctc tcgccgagat acgctggacg
    11581 gcttatttga gcccggagat gtactcggcc accgccgcga tctccgcatc gctcatgcgt
    11641 gccgccaccc cgcgcatcat ggacgcggga tcgttggcgc gctcgccgct gcggaaagct
    11701 ctgagctgcg ctgcggtgta ttcgccgtgc tgccccttga gccgcggata ctgcacgggg
    11761 atgccggccc cgttgggcga atgacagccc gagcaggcgg ccacgcccga tgcgctgttg
    11821 ccgccgcggt agagggcgcg cccggcgctc gccaatctcg cgtcgcgcgc gaagccgggc
    11881 cgggacccct gcgccgcgta gtacgcggcg acgtcgcgcg cgtcggcgtc ggacagagtc
    11941 gtggcgatag cggtcatgat cgggttgttg cgctcccccg acttgtagtt ctggagctgc
    12001 ttgacgaggt attcggcatg ctgtccggcg aggttcggat tggccccgat cgggctgttg
    12061 ccgtcgccgc cgtggcaggc tgcgcacacg ccggcggcga tcgtctgccc cctggccgcg
    12121 tctccggctc tcgccgcatc cggctgggcg gcggcgggcg acgccgccag tgccgcggcg
    12181 agggccgccg ctgcgagatg cttcatgctg actcccgaac ggtaagggcc gcgcccggcc
    12241 cgaacaggcg cgcattctag cctagggaag ctctgaacag gtatcgcact gtgcgttgcg
    12301 tcaggggcgg atgcgatact tgttcagagc ttccctagtc accaaccatg ctggagaagg
    12361 cgcagttccg aatcggcgcc gccgactggt cgcagcttcc gcccgatcac ggccgcgagg
    12421 tggcgtttgc cgggcgttcc aacgcaggca agtcgagcgc gatcaacgcc atcaccaatc
    12481 gccggcgcct tgctttcgtg agcaagacgc cggggcgtac ccaacaggtc aacttctacg
    12541 ccctgggcgc cgggcgccac ctggtggatc tgcccggcta tgggtacgcc cgcgtcgcgc
    12601 ccgaactgca gcagcgctgg gcgcggatgc tgagcgcgta tctgcagaat cgcgcagcgc
    12661 tgcgcgggct ggtgctgatc atggacgtcc gccgcccgct cacgccgctc gatcgtgcgc
    12721 tgctcgactg gttcgcgccg accgcaaagc cggtgctggc gctgctcacc aaagccgaca
    12781 agctctcccg gcagcaggcg acgcgacagc tcgacgcggt gtgcgcgatg ttcgagcgat
    12841 cgtatcccgg ggcgcgcgcg atccttttct cggccgtctc gcgcctgggc gtcgaggcgg
    12901 cgcaagggtt cgtcgcgggg ttgttggggg gacagggtga agcgcccgcg gagtacaatt
    12961 ccggcgcgca aagaaaagcc cccggctaaa ggggaaaaac cgggggcaaa agcctcaatt
    13021 ttggattaag gcacccgctc agggaggaaa agcgggagac gaaaccgtct gtttctatga
    13081 gtcccggcgt gcacgggaag ttcccgaaac gttaaatacc gtaaccgcca tcgccagccc
    13141 catgccagtc ctcggcaagt atccgcagcg gcgcatgcgt cgcaaccgcc atgacggttt
    13201 ctcccggcgc ctggtccgtg agcacacgct tacgccagat gatctgatct atccggtgtt
    13261 cgtgatcgag ggcgaaggca acgcacagcc ggtggagtcg atgccgggcg tcagccgcct
    13321 cacgctcgac cggctgctcc ccatggcgga gcggtgcctg cggctgggcg tgcctgccct
    13381 ggccttgttc cccgccatcg agccgcggct caagacgccc gacggacgcg aggcgatcaa
    13441 tcccaagggc ctcattccgc gggtagtcgc tgggctgaag aagcgctttc cggaactggg
    13501 ggtgatgacc gacgtcgcgc tcgacccctt caccagccac ggccaggacg gggtgatcga
    13561 cgccgccggc tacatcctca acgacgagac cattgccatc ctgcagaagc aggtgctcac
    13621 tcaagcggag gcgggcgtgg acatcgtggc cccctccgac atgatggacg gccgagtcgg
    13681 ggcgctgcgg gcggcgctcg atgcgcaggg cttcatccat acccgcatca tggcctatgc
    13741 cgcgaagtac gcgtccgcgt tctacgggcc gttccgggac gccgtcggct cggccgccaa
    13801 tcttggccaa ggcgacaagc tcacctacca gatggacccg gccaacggcg acgaagcgtt
    13861 gtgggaggtg ggcctggact tggaggaagg cgccgacatg gtgatggtca agcccggcct
    13921 gccctacctg gacatcgtgc gcagagtgaa ggacgcgtac cgggctccca cgttcgtcta
    13981 ccaggtgagc ggcgagtacg cgatgctgaa ggcggctttc cagaacggct ggctggacga
    14041 gcgcaaatgc gtgctggagg cgctgctcgc tttcaagcgt gccggcgcgg acggcatctt
    14101 gacctatttc gccctcgatg ctgcagagtg gctgcgcaac cgctgaacgc gcgtcacgag
    14161 tactgctgct aaccgcaaag gcgcaaaggc gcagaggaaa cgcagagaaa atcggatgct
    14221 cgtggcaggc actcgattgc ctttgcgcgt gcttcgcgcc tttgcgcctt tgcggtgaag
    14281 acttgtatta cccgcctgcg accagcgtct ccaccgacgc gatcggcgcg cgctccaggg
    14341 gccgaccgtc gagcgacagg cgcagcggct ggcgacgttg caccggcgac agcacggt
//
LOCUS       DASPGE010000081         3256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1548337, whole genome shotgun sequence.
ACCESSION   DASPGE010000081 DASPGE010000000
VERSION     DASPGE010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548337"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VD791_02530"
     CDS             <1..549
                     /locus_tag="VD791_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06878.1"
                     /translation="TARRALTADPAWDGKQFTGYPERGWRAWTGVMSVLASRTPRGLA
                     DTFLQPLDALEWMDKLTAENKAGGFDATDWLYQSWAYQEHDVGATPGFHGDLEAALGA
                     IQARTLILAPPLDLLNPVLCAQEAADLIPGAMLVEIPSVQGHQSAGTASAEDAVFLNE
                     EISAFLASIPSPEQEPGSSATG"
     gene            615..1025
                     /locus_tag="VD791_02535"
     CDS             615..1025
                     /locus_tag="VD791_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06879.1"
                     /translation="MTARIAVTLSALTLLAGTAVHAQEPGSPESLAQAKTGEEARLDL
                     QTIRKFGQSQGRFEVVVAWADSSRPAPEDYGPRQVRYMANCDEGTIALAAIGVFDRSG
                     QLTKTMIIPPGGADPVKPEKGSQEAKWLQRVCMF"
     gene            1173..1637
                     /locus_tag="VD791_02540"
     CDS             1173..1637
                     /locus_tag="VD791_02540"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXV06880.1"
                     /translation="MLRLCTMLAAAALALPVLADDHKIEYLKPPGLLNVPSFSQVTTA
                     SEGKLVFVSGQVSWDEKGKPLHAGDLEAQTRKTYENLKIALAAAGATFRDVVKFTVYV
                     KNLDTAKWRLISKVRSEYLSPDHPPASTMIGVTGLVYDELLIEIEAYALVSD"
     gene            complement(1634..1957)
                     /locus_tag="VD791_02545"
     CDS             complement(1634..1957)
                     /locus_tag="VD791_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06881.1"
                     /translation="MQRQADAAPEPIDAEMALLAYLLHKGFEEARIGLAQTERVLLEC
                     ADRLCSGALDEQELHEALERCRYALEHARQRDAYFVTRRHGSAEHFLVAAQQLLSGRD
                     DAGRS"
     gene            2090..>3256
                     /locus_tag="VD791_02550"
     CDS             2090..>3256
                     /locus_tag="VD791_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06882.1"
                     /translation="MKIAKREASTVQEGASALSLAPLGIDEWMCDARRVGAQVSLAWS
                     RHIALPRWRAVASSDSDLIGALVLFCGDSLHPFEPQRTGFAKMCYGFRREVIVLGVRR
                     SDRAVLELVPRAAGDEERRLCHAAANAPDFVLSAVLGVGRWRLSDSWRGFLVRWFSHG
                     FGMAAVQHAHMCRVMLDRVDPRALPQPLDKIEAELNRREIGVMRRARRRLLEWLDPEV
                     VRSVRRSNAILTPATYNLVATAAGEARERRVAALSTYPSVLPAFIAKDPPIPGNADVL
                     AYYESGSEHEFRALARFIVGAVDRGEPLSPYLSRPFDTRPQVIQAVRDVPRLVLWRIG
                     AWGLHALLRAVDAVPAGVRPTSPADWVAFRDIAVAVAALTEAFEDFSEEAGAQFW"
BASE COUNT          487 a         1136 c         1143 g          490 t
ORIGIN      
        1 acggcgcgac gtgcgctcac cgccgatccg gcctgggacg gcaagcagtt caccggctac
       61 ccggagcgcg gctggcgcgc ctggacgggc gtgatgagcg tgctggcgag ccgcacgccg
      121 cgcggcctgg cggacacgtt cctgcagccg ctcgacgcgc tggagtggat ggacaagctc
      181 accgccgaga acaaggccgg cggcttcgac gcgaccgact ggctgtacca gagctgggcc
      241 taccaggagc acgacgtggg tgccacgccc ggcttccacg gcgacctgga ggcggccctc
      301 ggggcgatcc aggcgcgcac cctgatcctg gccccgccgc tcgacctgct caacccggtg
      361 ctgtgcgccc aggaggcggc cgatctcatc ccgggcgcga tgctggtgga gatcccttcg
      421 gtccaggggc accagtcggc cgggaccgcg agcgccgagg acgccgtctt cctgaacgag
      481 gagatctcgg cgttcctggc ctcgatcccg tcgcccgagc aggagcctgg ttcgtccgca
      541 accggctagc gggaacgcaa tatgctctcg ccgctccgat gcagtagcat gcccattttt
      601 ctccgggagc tcacatgacc gcacgcatcg cagttaccct ctccgccctg acgctgctcg
      661 ccggcaccgc cgtccacgcc caggagccgg gcagccccga gagcctggcg caggccaaga
      721 ccggcgagga agcccgtctc gacctgcaga cgatccgcaa gttcggccag agccagggcc
      781 gcttcgaggt ggtggtggcc tgggccgaca gcagccggcc ggcgcccgag gactacggcc
      841 cgcgccaggt gcgctacatg gccaactgcg acgagggcac gatcgccctt gccgccatcg
      901 gcgtgttcga ccgcagcggc cagctcacca agacgatgat catcccgccc ggcggcgccg
      961 acccggtcaa gcccgagaaa ggcagccaag aggcgaaatg gctgcagcgg gtctgcatgt
     1021 tttgacctag tgcgccgagt gcgcccttaa ggcgcgattt aagaacctac tccctggcgc
     1081 caaccaggcg ggtggcgaag tctgcgccgg gcacaggcgc gggggagggc cgtggggtat
     1141 cctgcacgcg ctcaccccga tggaggcccg ccatgctccg cctgtgcacg atgctcgccg
     1201 ccgccgcgct cgctctgccc gtcctggccg atgaccacaa gatcgaatac ctgaagccgc
     1261 cggggctgct caacgtcccg agcttctccc aggtgaccac cgccagcgag ggaaagctgg
     1321 tgttcgtgag cgggcaggtg tcctgggacg agaagggcaa gccgctgcac gccggcgacc
     1381 tggaagcgca gacccgcaag acctacgaga acctgaagat cgcgctggcc gcggccggcg
     1441 caaccttccg cgacgtggtg aagttcacgg tgtacgtcaa gaacctcgac accgcgaagt
     1501 ggagactgat ctcgaaggtg cgcagcgaat acctgtcccc ggaccatccg ccggcaagca
     1561 ccatgatcgg cgtcaccggg ctggtgtacg acgagctgct gatcgagatc gaggcctacg
     1621 cgctggtaag cgactaactg cgcccggcgt cgtcgcgtcc gctcagcagt tgctgcgcgg
     1681 cgaccaggaa atgctccgcg ctgccgtggc gcctggtcac gaagtacgcg tcgcgctggc
     1741 gcgcgtgctc gagcgcgtag cggcaacgct ccagcgcttc gtgcagctcc tgctcgtcga
     1801 gcgcgccgct gcacagacgg tctgcgcact cgagcaacac gcgctcggtc tgcgccaggc
     1861 cgatgcgcgc ttcttcgaag cccttgtgca ggagataggc gagcagcgcc atctccgcat
     1921 cgatcggctc cggtgccgca tctgcttgac gttgcattga cagtccccgc gcgtgactcc
     1981 tgcccgagac acgcaattcc tgtgcgggag cgcgcggcgc gtgcgccgcg cgcgcagcag
     2041 cgatggcatg cagcttgcgt ccgggccctc ggatggggca ggcatgcgga tgaagatcgc
     2101 gaagcgcgaa gcaagcacgg ttcaagaggg cgcaagcgcg ctgtcgctgg ccccgctggg
     2161 catcgacgaa tggatgtgcg acgcgcgccg cgtcggcgcg caagtcagcc tggcctggtc
     2221 gcggcatatc gcgctgccgc gctggcgcgc cgtagcgagc tccgattccg acctgattgg
     2281 cgcgctcgtc ctgttctgcg gcgacagcct gcatccgttc gagccgcagc gtacgggttt
     2341 cgcgaagatg tgctacggct tcaggcgcga ggtcatcgtg ctcggcgtac gccgctccga
     2401 tcgtgccgtg ctggagctgg ttcccagggc ggcgggcgac gaggagcgcc gcctgtgcca
     2461 tgccgccgcc aacgcacccg acttcgtgct gtcggcggtg ctcggcgtag gccgctggcg
     2521 tctgagcgac agctggcgtg gcttcctggt gcgctggttc tcgcacggct tcggcatggc
     2581 ggcggtccag catgcgcaca tgtgccgcgt gatgctcgat cgcgtcgatc cgcgcgcgct
     2641 gccgcagccg ctggataaga tcgaagcgga gctgaaccgg cgcgaaatcg gcgtgatgcg
     2701 gcgcgcacgc cgccgcctgc tcgagtggct cgatcccgag gtggtgcgca gcgtgcgccg
     2761 cagcaacgcc atcctgacgc cggcgaccta caacctggtg gccaccgccg cgggcgaagc
     2821 gcgcgagcga cgcgtcgcgg cgctcagcac ctacccgagc gtcctgcccg cgttcatcgc
     2881 caaggacccg ccgattcccg gcaatgccga cgtgctggcg tactacgagt cgggcagcga
     2941 gcacgagttc cgcgcgctcg cccgcttcat cgtcggtgcc gtggaccgcg gcgagccgct
     3001 gtcgccgtat ctgtcgcggc cgttcgacac ccgcccgcag gtcatccagg cggtgcgcga
     3061 tgtgccgcgg ctggtgctgt ggcggatcgg cgcctggggg ctgcacgcgc tgctgcgcgc
     3121 cgtggacgcg gtgcccgccg gcgtgcggcc gacgtcgccc gcggactggg tcgcttttcg
     3181 ggacattgcc gtggcggtgg cggcgctcac cgaggcgttc gaggatttct ccgaggaggc
     3241 gggcgcgcag ttctgg
//
LOCUS       DASPGE010000082         6531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_494770, whole genome shotgun sequence.
ACCESSION   DASPGE010000082 DASPGE010000000
VERSION     DASPGE010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494770"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VD791_02555"
     CDS             <1..495
                     /locus_tag="VD791_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXV06883.1"
                     /translation="CHGERKFAKLLELVLADRLRRSDGQPSAMMPMSHSDPDDNLSSW
                     PFPHVYEIFMRIKEKEAPRHEHRNTVESVELLLQYDTFAYARRILNTPTLVVVAEQDN
                     ITQWDLEIQMYNTIPAGNKELVVLEKVNHMSLYSTRKHLEMASAHHARFLKKHLIDEF
                     TAEA"
     gene            492..1379
                     /locus_tag="VD791_02560"
     CDS             492..1379
                     /locus_tag="VD791_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010808944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV06884.1"
                     /translation="MTATLLLQSALAGLTNGFVYALVGLGIAVIFRGTRIVNAMQGEA
                     ALVGGLVAYLVLERLGLPLALAAVGGVLGGGVLGLATEVLLVRPTRRRGGTEESYLLL
                     TLGVAFAISAGVLYLFGRDSRLLPGIGGEASALIFDAAMRIHALWLIVIASAVMLLLW
                     LFYHRTHIGLSMMAASIDEEGAAAVGIDVPRMRTLTFLLGGLVGGLAGVLVSPLTTIH
                     YEMGLLLTLKGFAAAILGGLTNPFGAVVGGVTLGLLEAMAVLTVSSGYKDVIAMSILI
                     AIMILMPNGMLGRGARKGG"
     gene            1493..2479
                     /locus_tag="VD791_02565"
     CDS             1493..2479
                     /locus_tag="VD791_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV06885.1"
                     /translation="MSVQVRGARWRAMLGWLAALFALALWAGVPAFASRSIVDMLVFA
                     GLYTIAGLGVSFLLGQCGIVSLAQSVFYGIGAYSTAFCTTHLGWPAPAGFAAGMALSG
                     GVALAVGWPVLRLSGYFLALATLALAIIGHVLFTEWDWLTGGTLGIGGIPRLGLFGLP
                     LDSPQRFYYLVWPVAGLLLCLHRNLLNSRTGFAMRAMRDAPAAAAVLGVDIHALKVKV
                     FVLSALLGALAGSLFAHYVSFVSVDSFGVDRAIGFLLLAVLGGARTIAGTVLGALFIT
                     VMPHALSGFGDIHAVLFALFLILAVMFLPEGFGGALAGRLARRRAARSSAAG"
     gene            2482..3237
                     /locus_tag="VD791_02570"
     CDS             2482..3237
                     /locus_tag="VD791_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV06886.1"
                     /translation="MADILEVRDVSRSFSGLRVLNGVSLTVEQGSITGLIGPNGAGKS
                     TLFNIVSGFLEADAGAVIYAGMQIGRLSVPRRSRLGLVRSFQTPQVFAHLSVRENLMA
                     GCYKHTRSGMLANLLGTPGSRTELARMQREAQAACERFGLTEVSERPAGKLPAGQQRL
                     VELARACVGRPRLLCLDEPSSGLNSEEVAQLMQSLQRLNREGMSILLVSHDMELVAVA
                     SMVHVLCFGEIIASGPLEQIKAHARVREAYLGT"
     gene            3240..3977
                     /locus_tag="VD791_02575"
     CDS             3240..3977
                     /locus_tag="VD791_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010808940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV06887.1"
                     /translation="MLRVEGIEAGYGGLPVLRGVSLAIDRNEAVAIIGANGAGKSTLV
                     RAICGLLPVDAGRIVHHEREIQALPAHARSQHGVAVVLENRHLFGELSVRNNLTLAAI
                     HGARRGAADRRFHLHDVLELFPFMRARLDAPVQLLSGGEQQMVAIARALLLHPELLIM
                     DEPSTGLSPKVVRDIVGVMARLREGGMSILLVEQNVALAAETSERGYVMSLGRVVHEI
                     RRGEWDAFRNDERLLRAYLGGERADTA"
     gene            4035..5690
                     /locus_tag="VD791_02580"
     CDS             4035..5690
                     /locus_tag="VD791_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXV06888.1"
                     /translation="MQTDISGWVTRLCDEDIVRYTASGAWRDVTVADCAARLAQRAPD
                     RVGVLEGERSVSFRRLFDEAQRLAAALRGRGLQPGDVMSFQLPNWVETLVINLAACLA
                     GLVANPIVPIYRDAELRHILRDARSRLFFVPASFRGFDHAAMAQRLRPALPMLREVVV
                     VRGEAPGCTPYPDLLACARSGPPPEGVDPNAVKLLLYTSGTTGSAKGVLHSHNTIMSE
                     IDAVIGFWRITDRDVVLMPSPVTHITGYLYALETAFAAGVKTVLMERWNAAEAIELIT
                     RHGVTFSVGATPFLKELVAEAEARQAALSSLRLFMTGGAPVPPEIVYRAQRVLPGCMT
                     FRVYGSSEAPTITLGIRSRAENDLGATTDGRIVNHEVRLADPMTGAPVQGDADGEICT
                     RGPELMLGYTDWRQTQESFDADGYFRTGDLGHCVAGHYLCVTGRKKDLIIRGGENISP
                     KEIEDVLHTHPAIREAAVVAMPHERLGEGVCAFVIPCPGHVIDVASAAAHLERAGLAR
                     QKFPERVELVEELPRTASGKVQKNVLRERIAAQIAREKRGRGA"
     gene            6009..>6531
                     /locus_tag="VD791_02585"
     CDS             6009..>6531
                     /locus_tag="VD791_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV06889.1"
                     /translation="MYTSIVLEEASKVKNQNFAMRRLVAALALAGLAGAAYAGPVTDS
                     MLDAQAGESWLHTNGNYAGWRYSTLSQINSATAKRLKVAWMMSLGGKNDAQSTPSVHD
                     GVVYLPQDNKVFAVDAGTGRVIWKYEHKLPDDWGGYNVGFITGKHRGVALAGGNVYFL
                     SNDAKLHSINAKTG"
BASE COUNT          951 a         2188 c         2335 g         1057 t
ORIGIN      
        1 tgccacggcg agcgcaagtt cgccaagctg ctggagctgg tgctggccga ccgcttgcgc
       61 cgctccgacg gccagccgag cgcgatgatg ccgatgtcgc acagcgaccc ggatgacaac
      121 ctgtccagct ggccgttccc gcacgtgtac gagattttca tgcgcatcaa ggagaaggaa
      181 gcgccgcgcc acgagcaccg caacacggtc gagtcggtcg agctgttgct gcagtacgac
      241 accttcgcct acgccaggcg catcctgaac acgcccacgc tggtggtggt cgccgagcag
      301 gacaacatca cgcaatggga cctcgagatc cagatgtaca acaccatccc ggcgggtaac
      361 aaggagctgg tggtgctgga gaaagtcaac cacatgagcc tgtactccac acgcaagcac
      421 ctggagatgg cctcggcgca ccacgcgcgc ttcctgaaga agcatttgat cgacgagttc
      481 acggcggagg cgtgaccgcc accctgctcc tccaatccgc gctcgccggg ctcaccaacg
      541 gcttcgtcta cgcgctggtg gggctgggaa tcgcggtgat cttccgcggt acccgtatcg
      601 tcaacgcgat gcagggcgag gccgcgctgg tgggcggcct ggtcgcctac ctggtgctgg
      661 agcggctcgg cctgccgctg gcgctggccg cggtgggcgg cgtgctgggc ggcggcgtgc
      721 tggggctggc caccgaggtg ctgctggtgc gccccacgcg ccggcgcggg ggcaccgagg
      781 agagctacct gctgctcacg ctgggcgtgg ccttcgccat ctcggccggc gtgctctacc
      841 tgttcgggcg cgattcgcgg ctgctgccgg gcatcggcgg cgaagcttcg gcgctgatct
      901 tcgacgccgc catgcgcatc cacgcgctgt ggctgatcgt gatcgcctcc gccgtgatgc
      961 tgctgctgtg gctgttctac caccgcacgc acatcggcct gtcgatgatg gccgcctcga
     1021 tcgacgagga aggggcggct gcggtcggca tcgacgtccc gcgcatgcgc accctcacgt
     1081 tcctgctcgg cgggctggtg ggcggactgg ccggcgtgct ggtctcgccg ctcaccacga
     1141 tccactacga gatgggcctg ctgttgacgc tcaagggctt cgccgcggcg atcctgggcg
     1201 gactcaccaa cccgttcggc gcggtggtag gcggcgtgac cctcgggctg ctcgaggcga
     1261 tggcggtgct gacggtgtcc tcgggctaca aggatgtcat cgcgatgagc atcctgatcg
     1321 cgatcatgat cctgatgccc aacggcatgc tgggccgggg cgcgcgcaag ggcggctgac
     1381 cggaaccgga gcgcggtgca tgagcgaacc gcccgatgta tgagcaacgc ccccggtgta
     1441 tgagcaacgc cctggctgta tgagcaaccc cttgggacga gctccggact cgatgtccgt
     1501 ccaggttcgc ggcgcgcgtt ggcgcgcgat gctgggctgg ctggcggcgc tgttcgcgct
     1561 cgcgctttgg gccggcgtgc ccgccttcgc ctcgcgcagc atcgtcgaca tgctggtgtt
     1621 cgccggcctg tataccatcg ccggcctggg cgtgtccttc ctgctcggcc agtgcggcat
     1681 cgtgtcgctg gcgcagagcg tgttctacgg tatcggcgcc tacagcacgg cgttctgcac
     1741 gacccatctg ggctggccgg cgccggccgg tttcgccgcc ggcatggcgc tgagcggcgg
     1801 tgtcgcgctg gcggtcggct ggccggtgct gcgcctctcg ggctacttcc tggcactcgc
     1861 cacgctcgct ctagcgatca tcggccacgt cctgttcacc gaatgggact ggctcaccgg
     1921 cggcacgctc ggcatcggcg gcatcccgcg gctcggtctg ttcggcctgc cgctcgacag
     1981 cccgcagcgg ttctactatc tggtgtggcc ggtggcgggg ctgctgctct gcctgcaccg
     2041 caacctgctc aattcgcgca ccgggttcgc catgcgcgcg atgcgcgatg cgcctgcagc
     2101 agcggcggtg ctgggcgtgg acatccacgc gctcaaggtc aaggtgttcg tgctgagcgc
     2161 gctgctcggg gccctggcgg gcagcctgtt cgcgcattac gtctccttcg tcagcgtcga
     2221 cagcttcggc gtggaccgcg ccatcggctt cctgctgctg gcggtgctgg gcggcgcgcg
     2281 caccatcgcc gggaccgtgc tcggcgcgct gttcatcacc gtgatgccgc atgcgctgag
     2341 cggcttcggc gacatccatg cggtgctgtt cgcgctgttc ctgatcctgg cggtgatgtt
     2401 cctgcccgag ggtttcggcg gagcgctcgc cggccggctg gcgcggcgtc gagcggcgcg
     2461 ctcttccgca gcgggctgag catggccgac atcctggaag tgcgcgacgt ctcccgcagc
     2521 ttcagcggcc tgcgggtgct gaacggcgtc agcttgacgg tcgagcaagg cagcatcacc
     2581 ggcctgatcg ggcccaacgg cgccggcaag agcacgctgt tcaacatcgt gtccggcttc
     2641 ctggaagccg atgcgggcgc ggtgatctac gccggcatgc agatcggccg gctgtcggtg
     2701 ccgcggcgca gccgtctcgg attggtgcgc agcttccaga cgccccaggt gttcgctcat
     2761 ctgagcgtgc gcgagaacct gatggccggc tgctacaagc atacgcgcag cggcatgctc
     2821 gccaatctgc tcggcacgcc gggctcgcgc accgagctgg cgcggatgca gcgcgaggcg
     2881 caggcggcgt gcgagcgctt cggcctgacc gaagtgagcg agcggccggc cggcaagctg
     2941 cccgccggcc agcagcgact ggtcgagctg gcgcgggcct gcgtcggccg gccgcgcctg
     3001 ctgtgcctgg acgagccctc ctcgggcctc aactcggagg aggtggcgca gctgatgcag
     3061 agcctgcagc ggctgaaccg ggagggcatg tcgatcctgc tggtgtcgca cgacatggag
     3121 ctggtcgcgg tcgcctcgat ggtgcacgtc ctgtgcttcg gcgagatcat cgccagcggg
     3181 ccgctggagc agatcaaggc gcacgcacgc gtgcgcgaag cctacctggg gacctgaccg
     3241 tgctgcgcgt ggagggcatc gaggcgggat atggcgggct gccggtgctg cgcggagtat
     3301 cgctcgccat cgaccgcaac gaggcggtgg ccatcatcgg cgccaacggc gccggcaagt
     3361 ccaccctggt gcgcgcgatc tgcggcctgc tgccggtcga cgccgggcgc atcgtgcacc
     3421 acgagcgcga gatccaggcg ctgccggcgc acgcccgctc ccagcatgga gtggcggtgg
     3481 tcctggagaa ccggcacctg ttcggcgaac tgtccgtgcg caacaacctg acgctcgccg
     3541 cgattcacgg tgcacggcgc ggcgcggccg atcgccgctt ccacctgcac gacgtgctgg
     3601 agctgttccc gttcatgcgc gcgcggctcg atgcgccggt gcagctgctc tccggcgggg
     3661 agcagcagat ggtcgccatc gcccgcgcgc tgctgctgca ccccgagctg ctgatcatgg
     3721 acgagccctc caccggcctg tcgcccaagg tggtgcgcga catcgtcggc gtcatggcgc
     3781 gcctgcgcga gggcggcatg agcatcctgc tggtggagca gaacgtggcg ctcgccgccg
     3841 agacctcgga gcgcggctac gtgatgtcgc tgggtcgcgt ggtgcacgag atccggcgcg
     3901 gcgagtggga cgctttccgc aacgacgagc ggctgctgcg cgcctacctg ggcggagaac
     3961 gcgccgacac ggcctgaggc gccccctatc gtaaggcgac tgcctgagag gcgatcccga
     4021 gaagggcgac aacggtgcag accgacatca gcggctgggt cacccggctc tgcgacgagg
     4081 acatcgtgcg ctacacggca agcggcgcct ggcgcgacgt cacggtcgcc gactgcgccg
     4141 cgcgcctggc gcagcgcgcg ccggaccgcg tcggcgtcct cgaaggcgag cgcagcgtga
     4201 gttttcgccg gctgttcgac gaggcgcaac ggctcgccgc ggccctgcgc gggcgcggcc
     4261 tgcagccggg ggacgtgatg agcttccagc tgcccaactg ggtcgagacg ctggtgatca
     4321 acctcgcggc ctgcctcgcc gggctggtgg cgaatcccat cgtgccgatc taccgtgacg
     4381 cggagctgcg ccacatcctg cgcgatgcgc gctcgcgcct gttcttcgtc ccggcatcgt
     4441 tccgcgggtt cgatcacgcg gcgatggcgc agcgcctgcg gccggcgctg cccatgctgc
     4501 gcgaggtggt ggtggtgcgc ggtgaggctc cgggatgcac cccatatccc gacctgctcg
     4561 cctgtgcgcg cagcggccct ccgccggagg gcgtcgaccc caacgccgtg aagctgctcc
     4621 tgtacacctc gggcaccacc ggcagtgcca aaggcgtgct gcacagccac aacacgatca
     4681 tgtcggagat cgacgcggtg atcggcttct ggcgcatcac cgatcgcgac gtggtgctga
     4741 tgccctcgcc ggtgacgcac atcaccggtt acctgtatgc gctggagacc gccttcgcgg
     4801 ccggcgtgaa gacggtgctg atggagcgct ggaacgccgc cgaggccatc gagctgatca
     4861 cccgtcacgg cgtgaccttc agcgtcgggg ccacgccgtt tctgaaggag ctggtggcgg
     4921 aagcggaagc gcgtcaggcc gccctttcca gcctgcggct gttcatgacc ggcggtgctc
     4981 cggttccgcc ggagatcgtc tatcgtgccc agcgggttct gcccggctgc atgacgttcc
     5041 gcgtctacgg cagcagcgag gcccccacca tcacgctcgg catccgcagc cgggccgaga
     5101 acgacctggg cgcgaccaca gacggccgga tcgtcaacca cgaggtgcgc ctggccgatc
     5161 cgatgaccgg ggcgccggtg cagggcgatg ccgacggcga gatctgcacg cgcgggccgg
     5221 agctgatgct cggctacacc gactggcggc agacgcagga gagcttcgat gccgacggct
     5281 actttcgcac cggggatctg ggccattgcg tcgccggcca ctacctgtgc gtgaccggcc
     5341 gcaagaagga cctgatcatc cgcggcggcg agaacatcag ccccaaggag atcgaggacg
     5401 tgctgcacac tcaccctgcg atccgggaag cggcggtggt ggcgatgccg cacgagcggc
     5461 tcggcgaggg cgtgtgcgcg ttcgtgatcc cttgcccggg ccatgtcatc gacgtggcga
     5521 gcgccgccgc gcacctggag cgggcggggc tggccaggca gaagttcccc gagcgcgtgg
     5581 agctggtcga ggagctgccg cgtacggcca gcggcaaggt gcagaagaac gtgctgcgcg
     5641 agcgcatcgc tgcgcagatc gcgagggaaa agcgaggccg gggagcgtga cgctgtgctg
     5701 caatgcggga aaagcgccgc gcggctggcg cccaggcagt tgatgcacag ggaattcggg
     5761 agtagtgttg tgccgataca agtgccggcg aggtggcctc gccgtgaagg cactggaggc
     5821 ggcagcgcag acgccgctgc ggggaggagc atgtcgagtc tgtatgtcac atctccagcg
     5881 ctggccggcg gcttgccgcc gccgctgcgt cgcacgccgt gccgccaccc gattcgcccg
     5941 ccgcccgcag gcggccaccc ggttcgtgtt ctctcaggcc ctgtgtcctg ggaatgcgaa
     6001 gcaagcagat gtacacgtca atcgttctgg aggaggcatc gaaagtgaaa aatcagaact
     6061 tcgcaatgcg gcgcctggtt gcggcgctgg ccttggccgg cctggccggc gcggcctatg
     6121 cgggtccggt gacggactcg atgctcgacg cgcaggccgg cgagtcgtgg ctgcacacca
     6181 acggcaacta cgccggctgg cgctatagca cactgagcca gatcaacagc gctaccgcca
     6241 agcgtctgaa ggttgcctgg atgatgtcgc tgggcggcaa gaacgacgcg caaagcacgc
     6301 ccagcgtgca cgacggcgtg gtgtacctgc cgcaggacaa caaggtgttc gccgtggatg
     6361 ccggcaccgg ccgcgtgatc tggaagtacg agcacaagct gccggacgac tggggcggct
     6421 ataacgtcgg cttcatcacc ggcaagcatc gcggcgtggc cttggccggc ggcaatgtgt
     6481 atttcctgtc gaacgacgcc aagctccact cgatcaacgc gaagaccggc a
//
LOCUS       DASPGE010000083        15645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1431731, whole genome shotgun sequence.
ACCESSION   DASPGE010000083 DASPGE010000000
VERSION     DASPGE010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15645
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431731"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VD791_02590"
     CDS             complement(<1..581)
                     /locus_tag="VD791_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV06890.1"
                     /translation="MSEPLIVVPDDFPSVFKGTAAEERCRRLGETRVFVERGADGESE
                     LIRRLGGARIAINIRAHARFSDAVLDACPRLQMISVWGTGTDNIDLESAQRRGITVCN
                     TAGVNASAVAEHTIALMLAVARRIPRIDHEMRAGAWPREMLTQLLGKTLGVFGTGRIG
                     ARVVELGRAVGMTVLAWSRRGEPARITALGARPA"
     gene            717..1013
                     /locus_tag="VD791_02595"
     CDS             717..1013
                     /locus_tag="VD791_02595"
                     /inference="COORDINATES: protein motif:HMM:NF020469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HXV06891.1"
                     /translation="MLVKFESDVGSFTMFGDAAIALLKMMGHSGTVPSAILARDIPAA
                     LERLKSALAEQREPPPEPAGNESKEPRVPLRTRAWPLLDLLTRAAAKNADVMWR"
     gene            1004..1927
                     /gene="alkA"
                     /locus_tag="VD791_02600"
     CDS             1004..1927
                     /gene="alkA"
                     /locus_tag="VD791_02600"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF007641.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HXV06892.1"
                     /translation="MALSGFRRAPRRRAPRGDASQGPELRLRYRPPLDWSALLAFLAR
                     RAVAGVEHVADDVYLRTLRIEQDGVAHRGWIAVRPAHHDDAMRIALSPSLSRVSALVL
                     TRAGRAFDLSCDPGRVAAALGALGAGNPGLRVPGAFDGFEAAVRGILGQQVTVKAAHT
                     LAGRFAAAFGDPLDTAHPALRCAFPAPDRIAALRPARVAALGIVRTRAAAIVGIARAL
                     AQGRLRLDPTADVNATLEALRAIPGIGEWTAQYIAMRALAWPDAFPHSDYGVLKALGE
                     TDPRRALQRAEAWRPWRAYAVMHLWKSLEAA"
     gene            complement(1996..3486)
                     /locus_tag="VD791_02605"
     CDS             complement(1996..3486)
                     /locus_tag="VD791_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HXV06893.1"
                     /translation="MTPLPHGPDWEFELIERYHAAIQETAAEFALDTYANQIEVISSE
                     QMLDAYASSGLPLGYPHWSFGKAFIRNEQAYRKGYQGLAYEIVINSDPCIAYLMEENT
                     MVMQALVIAHACYGHNSFFKGNYLFRQFTQADAILDYLVFARRYVMECEQRHGMSEVE
                     EIIDSCHALMEYGVDRYRRPQPISAREKEARASQRAEHQRRLYNDLWRTLPQKEAAAE
                     AVDERPFPVEPEENVLYFVEKHSPRLAPWQRELTRIVRKLSQYFYPQVQTKVMNEGWA
                     TFWHHTLLNRMYEKRLVNDAFMLEFLHHHTNVVTQRGFDEKGYHGINPYALGLAMMQD
                     IRRICENPDEEDRHWFPDLAGSDWRAQLDFAMRNFKDESFIAQYLSPRLIRELRLFVV
                     ADHEGEAELTVDSIHDEYGYRRVRRMLAEQYRRDNRLPDIQVARYQRDTDRSLVLRNQ
                     LYRGRPLADAEADEVLRHLGRLWGFPVRLESVDAEGKVQGTREWRP"
     gene            complement(3483..4751)
                     /locus_tag="VD791_02610"
     CDS             complement(3483..4751)
                     /locus_tag="VD791_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HXV06894.1"
                     /translation="MMYLIDRRLNGGNKSAVNRERFMRRYKEHIRRAVKGMVADRSIR
                     DMIQGGEVKVPAKDISEPGFRYGQGGDWETVHPGNREFVRGDRIPRPSGGPGGGGSEP
                     GTGESTDDFVFTLSREEFLNIFFEDLELPNLMRTTLAEAEHKKLVRAGYVKDGTPANL
                     SVPRTMKTALARRIALSGPIKDDIERLEGLLAASVRSGVPQGRIQALEAEILELEGRL
                     QRLPFLDDVDLRYRHRVAQPAPTARAVMFCLMDVSASMDERKKDLAKRFFTLLYLFLT
                     RKYERVELVFIRHTDEAEEVDEDTFFYDQRSGGTVVLSALELMRKVMGERYRLSEWNV
                     YAAQASDGDAFGADAGKSARFLREELLPATRYFAYIETPEEEYSRKSPLWAEYEELRE
                     SSPNFAMKRVTRPQEIYPVFRDLFSKEKVG"
     gene            complement(5002..6927)
                     /locus_tag="VD791_02615"
     CDS             complement(5002..6927)
                     /locus_tag="VD791_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HXV06895.1"
                     /translation="MTSIIEGYKATYSAAQDEEMTLEQYLDLCRRDPGAYASAAERIL
                     QAIGEPDLVDTRNDPRLSRIFANRLIKRYPAFAEFYGLEDAISQIVSFFKHAAQGLEE
                     RKQILYLLGPVGGGKSSIAERLKQLMEKLPIYALKGSPVNESPLALFAPERYGDTLEK
                     EYRIPHRYLTGVMSPWAVKRLKEFDGDIGQFRVVRVQPSVLRQVAIAKTEPGDENNQD
                     ISTLVGKVDIRKLDRFAQNDPDAYSYSGGLCLANQGFLEFVEMFKAPIKMLHPLLTAT
                     QEGNFKGTEGFSAIPFNGIILAHSNESEWQSFRNNRTNEAFLDRIYIVKVPYCLQVSE
                     EVKIYEKLLRHSALAHAPCVPGTLDMMAQFSVLSRLKEPENSSLYSKMRVYDGESLKD
                     TDPKAKSFQEYRDYAGPDEGMSGISTRFAFKVLSAVFNYDQTEVAANPVHLMYVLEQR
                     IGREDFPEEQKRKYLEFIKGWLSPRYAEFIGKEIQTAYLESYSEYGQNIFDRYVTYAD
                     CWLQDEEYRDPDTGEIFDRAALNNELEKIEKPAGIANPKDFRNEVVNFVLRARANNAG
                     RNPAWSSYEKLREVIEKKMFSSTEDLLPVISFNAKASAEDQEKHQSFVARMVSKGYTE
                     KQVRLLSEWYLRVQKSS"
     gene            7740..8708
                     /locus_tag="VD791_02620"
     CDS             7740..8708
                     /locus_tag="VD791_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06896.1"
                     /translation="MKTTRVAVAAALLLASALLPVSTELRAYQIIEGRYRVEVSPGVF
                     EDQLVVRCDDGRTLTVRWETKLAEACGESLMGGPAPKAARAAAPSPAPKAAQSPQAAP
                     QTKTAPEPPPAAATPAGLLDEQSQKDAMLAQVRAQFGDVPEHYVEFKPGPDGLSMRFL
                     PPLSDILKKYEMCRKVRETRADCAADRDNAIAKLSATRPPAAAGTDKAVDKANPAVAG
                     DDKSLAPPAKAAAPSETPAPAAASPQAHAAPAQPDAAAAAAADRVAAEQKILEEYTWC
                     MRAKPKFECEQARAKALSALDKPKPAAKPKRAPKQAAAPNVAATAR"
     gene            8897..9913
                     /locus_tag="VD791_02625"
     CDS             8897..9913
                     /locus_tag="VD791_02625"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXV06897.1"
                     /translation="MPRARLAPFASPLFAALLFAAPLFAALGLAAPLSAAAASFALPS
                     SGDDLVGELSVVVAREEDTLLDIARQHDLGFNEIAAANPDIDPWLPGAGARVVLPLQF
                     VLPAGPRSGIVINLEQMRLFYFPESGANAGRVVTHPLGVGVEHGATPLGQTRVIRKAV
                     DPVWRPPQSIRAARAAAGETLPEVVPPGPDNPLGRHALYLGFPGYLVHGTNRPWGIGM
                     RVSGGCIRLYPEDIESLYAQVPPGTAVRIVSEPFAVGLLRGMPYVQVFARPERDADNR
                     SYTPLVEGLIRAAPNAALDWGRVLRTAKEQRALAIPVGRSAPTLEQLISGAQRAAAAP
                     PQER"
     gene            complement(9970..10371)
                     /locus_tag="VD791_02630"
     CDS             complement(9970..10371)
                     /locus_tag="VD791_02630"
                     /inference="COORDINATES: protein motif:HMM:NF023267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine-zipper protein"
                     /protein_id="HXV06898.1"
                     /translation="MNRRSTITRMSLLALPFALAQGCGTTGGAGAAGAQTAAGPDRVE
                     QALATAQSALNEARAARAAAEAANAKADQILTTVRAMPVSGDMSQEVLQASRAAQAAA
                     EDAKRTALEAQDAVERLDIKTDRMFEKSMRK"
     gene            10548..11483
                     /locus_tag="VD791_02635"
     CDS             10548..11483
                     /locus_tag="VD791_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXV06899.1"
                     /translation="MSAFSSARRPVDATGAALMTLLCLLWGFTYVFSKLAAADISLVL
                     QGGLRSVIAALLLLVWARARGIPLFSRDGTLGAGLAAGVLFAAEFLFIFAGLAHTGAS
                     RMVVFIYLAPCFTALGLQWLVPGERLHARQWAGIALAFVGVAAAFADGFASERASLLG
                     DAFGVIAAALWAATTVIIRSTRLNNANAAKTLFYQLAVAGVALPPASLLLGEPGVVAL
                     TPIALVSLLYQGAVISFASYLIWFWLLTRYLAARLSVFTFLSPLFGVTASVAVLSEPL
                     RPAFVAAVALVGAGIYLVNRPAPAHALRSPPPRRP"
     gene            complement(11428..12861)
                     /locus_tag="VD791_02640"
     CDS             complement(11428..12861)
                     /locus_tag="VD791_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HXV06900.1"
                     /translation="MPALDSRALSAFVDRKWNEDIVPRLVEYIRIPCKSPHFDAQWQG
                     NGHIEAAIGLAHRWCSAQQVPGMQLEILRLPGRTPVLYLDVPASGAGAQGTVLIYGHL
                     DKQPEMVGWRAGLGPWSPVVEDDRLYGRGGADDGYAVFAALAALTALRAQGHPHARVA
                     GLIECCEESGSYDLPAYLEALTPRLGKVDLVIGLDSGCGNYEQLWVTTSLRGLAAGTL
                     GVEVLAEGVHSGDASGVVPSSFRVARMLLDRLEESATGRVRPPELHCQIPPERVEQAR
                     QAGEILRESVYAKFPFAGGTQPLTTDFTQAILNRTWRPFLSVVGADGMPAIRDAGNVL
                     RPGTALKLSLRLPPPVDAQRATALMRELLEADPPHGARVRFEPDQAATGWCAPATAPW
                     LERALQGASRAHYGRPAAAMGEGGTIPFMGMLGRVFPGAQFLITGVLGPHSNAHGPNE
                     FLHIPYARKLTACVAEVIAAHALAPAG"
     gene            complement(12937..14439)
                     /locus_tag="VD791_02645"
     CDS             complement(12937..14439)
                     /locus_tag="VD791_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06901.1"
                     /translation="MKSASSATRQPEGAVPKAPPLPIVLPTADPLGAMEEAAFWLEAY
                     GQNPSSDPLRIFEVIDRLDALVQPHRLALLHEYLESGGRMQTYREQRLWQAAARYARH
                     LADGYSACLRLSQGQGERKLVPVLAARAVRAHTLELRWALLRYSAVDDEIWPRLGQLY
                     ALCDRAGLATVRFKIYPAMSGDSTVRREYLRALVLSVSAMGNLLPAAQAVAERVIAAV
                     AEFFLLHRRPAAGCHFAVDLKASRPPYRIGEGIAPARGVRFFGPGDAGVMVEGFLAQL
                     AAQRGVPAQLRLGGEFEPSLVREVLQHLARHWGAKPPTRGEARHRVLATAHVAHGFER
                     VLAAVGGEQAQDEVTEAWTVENESEGGFGALIPARDDDWLEVGRLVAAKPDWPSSWCV
                     GVIRRLTARSPMHRSVGVEILARGGVCVELAPLPLKEGLQPLHGILLPTEAQTSLAGG
                     EVELVLPRGSTARLASCELRMHCRLYRIERRRIADSGSDFEIARFALREA"
     gene            14562..>15645
                     /locus_tag="VD791_02650"
     CDS             14562..>15645
                     /locus_tag="VD791_02650"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXV06902.1"
                     /translation="MRESRSHVTIAGSMSAVTLTARPAVLQLRASRIREVANAAMGRE
                     DILAFWFGEPDEVTPEFIRRAAVESLERGETFYSQNLGLPELRQTLATYISRLHCPTT
                     ADQIAVTSSGLSALQLASQALAGHGERVVEVTPLWPNLVEIPKILGAQVECVPLRFDP
                     GGWRLDLDRLLDALTPRTRVLFVNSPNNPTGWTVGRAEQRAILEHCRRHGIWLVADDA
                     YERLCYDDDAGGGVAPSFLDLADPGDRVVSTNTFSKSWLMTGWRLGWMRGPAALIAEM
                     PKLIEYNTSCTPVFVQRAGVVAVSEGEPVVAHTLTRLRQARDFLLEQLARTPGIEVAR
                     PAGAMYAFFRVAGVHDSLALCKHLVAG"
BASE COUNT         2263 a         5491 c         5382 g         2509 t
ORIGIN      
        1 gccggccgcg cgccgagggc cgtgatccgc gccggctcgc cccgccggct ccacgcgagc
       61 acggtcatcc cgacggcgcg gcccagctcg acgacgcggg cgccgatgcg cccggtgccg
      121 aagacgccca gggtcttgcc cagcagctgc gtcagcatct cgcgcggcca cgcgcccgcg
      181 cgcatctcgt ggtcgatcct cggtatgcgg cgggccacgg cgagcatcag ggcgatggtg
      241 tgttcggcca cggcgctggc gttcaccccg gcggtgttgc acacggtgat gccgcgccgc
      301 tgcgccgatt ccaggtcgat gttgtcggtg ccggtacccc ataccgagat catctggagt
      361 cgcggacacg cgtccagcac ggcgtcgctg aagcgcgcat gcgcgcggat gttgatggcg
      421 atgcgtgcgc cgccgagtcg gcgaatgagt tcggactccc cgtccgcgcc ccgctcgacg
      481 aaaacgcgcg tctcgcccag gcgccggcag cgctcctctg ccgccgtgcc cttgaacacc
      541 gacgggaagt cgtcgggaac gacgatgagg ggctcgctca tggcccagaa tagcgccgat
      601 ccgcgggtcg cagcaaggcc gccgcgaacc gcggctgcgc gcgccactgg gggcgaaagg
      661 ccaccgcgac tgcggtatcc ttgcgcaacg tccgacggat ccgggagcca aagccgatgc
      721 tggtgaaatt cgagagcgat gtgggcagct tcaccatgtt cggcgacgcc gcgatcgcgc
      781 tgctgaagat gatgggccac agcggcacgg tgccgagcgc catcctcgcg cgcgacatcc
      841 cggccgccct ggagcggctg aagagcgcgc tggcggagca acgcgagccg ccgcccgagc
      901 cggcaggcaa cgaatccaag gaaccgcgcg tgccgctgcg cacgcgcgcc tggccgctgc
      961 tggatctgct gacccgcgct gcggcgaaga acgcggacgt gatgtggcgc tgagcggctt
     1021 tcgccgcgcg ccgcgccggc gggcgccccg gggcgacgcg tcgcaggggc ccgagctgcg
     1081 gctgcgctat cgaccgccgc tcgactggag cgcgctgctc gccttcctcg cgcgccgcgc
     1141 cgtggccggc gtggagcacg tcgccgacga cgtctatctg cgcacgctgc gcatcgagca
     1201 ggacggcgtc gcacaccgcg gctggattgc cgtccgcccg gcgcaccacg acgacgcgat
     1261 gcgcatcgcg ctgtcgccct ccctgtcgcg cgtgagtgcg ctggtgctga cgcgcgccgg
     1321 acgcgccttc gatctgagct gcgaccccgg ccgggtggcc gctgcgctcg gtgcgctggg
     1381 cgccggcaat ccgggtctgc gcgtgcccgg cgccttcgac ggcttcgagg cggcggtgcg
     1441 cgggatcctg gggcagcagg tgacggtcaa ggcggcgcac acgctggccg gacgcttcgc
     1501 tgccgcattc ggcgacccgc tcgacactgc gcatcccgcg ctgcgctgcg cttttcccgc
     1561 accggaccgc atcgccgccc tgagaccggc gcgggtggcg gcgcttggaa tcgtgcgcac
     1621 gcgcgccgcc gccatcgtcg gcatcgcacg cgccctcgcg caaggccgcc tgcggctgga
     1681 cccgactgcc gacgtgaacg cgacgctcga ggcgctgcgg gcgattccgg gcatcggcga
     1741 gtggaccgcg cagtacatcg cgatgcgcgc gctcgcctgg ccggatgcct ttccgcacag
     1801 cgattacggc gtgctcaagg cgttggggga aaccgatccg cgtcgcgccc tgcagcgcgc
     1861 cgaggcctgg cgcccctggc gcgcctacgc ggtgatgcac ctgtggaagt cgctggaggc
     1921 ggcctgaacc gctagcccgg atgtctcatc gggacgcgga attgcggccc ggggtggccc
     1981 ggtgaaagat ccgggctagg gccgccactc gcgcgtgcct tgcaccttgc cttccgcgtc
     2041 caccgattcc aggcgcacgg ggaagcccca tagccggccc aggtgacgca gcacctcgtc
     2101 ggcctcggcg tcggcgagcg ggcgcccgcg gtagagctgg ttgcgcagca ccagcgagcg
     2161 gtcggtgtcg cgctggtagc gggcgacctg gatgtcgggc agccggttgt cgcggcggta
     2221 ctgctcggcc agcatgcgcc gcacccgccg gtagccgtac tcgtcgtgga tgctgtccac
     2281 ggtgagctcg gcttcgccct cgtgatcggc caccacgaac aggcgcagct cccggatcag
     2341 gcgcggcgag aggtactggg cgatgaacga ttcgtccttg aagttgcgca tcgcgaagtc
     2401 gagctgcgcg cgccagtcgc tgccggcaag atcggggaac cagtggcggt cctcctcgtc
     2461 cgggttttcg cagatgcgac ggatgtcctg catcatggcg agccccagcg cgtaggggtt
     2521 gatgccgtgg tagccctttt cgtcgaaacc gcgctgcgtc accacgttgg tgtgatgatg
     2581 caggaattcc agcatgaagg cgtcgttcac caggcgcttc tcgtacatcc ggttgagcag
     2641 cgtgtggtgc cagaacgtcg cccaaccttc gttcatcacc ttggtctgca cctgcgggta
     2701 gaagtactgc gacagcttgc gcacgatgcg cgtcagctcg cgctgccagg gcgccaggcg
     2761 cggcgagtgc ttctcgacga agtacagcac gttctcttcc ggctccaccg ggaacggccg
     2821 ctcgtcgacc gcctccgccg ccgcctcctt ctgcggcagc gtgcgccaca ggtcgttgta
     2881 caggcggcgc tggtgctcgg cgcgctggct ggcgcgcgcc tccttctcgc gcgccgagat
     2941 cggctgcggg cggcgatagc ggtccacacc gtactccatc agcgcgtggc aggaatcgat
     3001 gatctcctcg acctcggaca tgccgtggcg ctgctcgcac tccatcacgt agcggcgcgc
     3061 gaacaccagg tagtcgagga tggcgtcggc ctgggtgaac tgccggaaca ggtagttgcc
     3121 cttgaagaag gagttgtggc cgtagcaggc gtgcgcgatg accagcgcct gcatcaccat
     3181 cgtattctcc tccatcaggt aggcgatgca cggatcggag ttgatgacga tctcgtaggc
     3241 caggccctga tagcccttgc ggtaggcctg ctcgttgcgg atgaaggcct tgccgaagga
     3301 ccagtgcggg tagccgagcg gcagcccgct cgaggcatag gcgtcgagca tctgctcgga
     3361 gctgatcacc tcgatctggt tggcgtaggt gtccagagcg aactcggcgg cggtctcctg
     3421 gatcgcggcg tggtagcgct cgatcagctc gaactcccaa tccggcccgt gcggcagcgg
     3481 ggtcacccga ctttctcttt gctgaataag tcgcggaaca ccgggtagat ctcctgcggc
     3541 cgggtgacgc gcttcatggc gaagttgggc gagctctcgc gcagctcctc gtactcggcc
     3601 cacagcgggc tcttgcgcga atactcctcc tccggcgtct cgatgtaggc gaagtagcgc
     3661 gtggccggca acagttcctc gcgcaggaag cgcgcgctct tcccggcatc ggcgccgaag
     3721 gcgtcgccgt cggaggcctg cgcggcgtag acgttccatt cgctcagacg gtagcgctcg
     3781 cccatcacct tgcgcatcag ctccagggcg gagagcacca ccgtaccgcc gctgcgctgg
     3841 tcgtagaaga aggtgtcctc gtccacctct tccgcctcgt cggtgtggcg gatgaagacc
     3901 agctcgacgc gctcgtactt gcgcgtgagg aaaaggtaca ggagcgtgaa gaaacgtttg
     3961 gccagatcct tcttgcgctc gtccatggag gcggacacgt ccatcaggca gaacatgacc
     4021 gcccgggcgg tgggcgcggg ctgcgcgacg cgatgccgat agcgcagatc gacgtcgtcc
     4081 aggaacggca ggcgctggag ccgcccttcg agctccagga tctcggcctc cagcgcctgg
     4141 atgcggccct ggggcacgcc gctgcgcacc gaagcggcga gcaggccttc gagcctttcg
     4201 atgtcgtcct tgatcggccc gctcagcgcg atgcgccggg cgagcgcggt cttcatggtg
     4261 cgcggcacgc tcaggttggc cggcgtgccg tccttgacgt agccggcgcg caccagcttc
     4321 ttgtgctcgg cctcggccag cgtggtgcgc atcaggttgg gcagctcgag gtcctcgaag
     4381 aagatgttga ggaactcctc gcgcgacaac gtgaacacga aatcgtccgt gctctcgccc
     4441 gtgcccggct cgctcccgcc gccgcctggc ccgccgctgg gccgcggaat gcggtcgcca
     4501 cgcacgaact cgcggttgcc cggatgcacc gtttcccagt ccccgccctg gccgtagcgg
     4561 aagccgggct cggagatgtc tttggccggc accttgacct cgccgccctg gatcatgtcc
     4621 cggatcgagc ggtcggccac catgcccttg accgcgcgcc ggatgtgctc cttgtagcgg
     4681 cgcatgaagc gctcgcggtt gaccgcgctc ttgttgccgc cgttcaggcg gcggtcgatg
     4741 aggtacatca tgatgggaac tgggcggaga ggccggggct gccgaaaggc gtgatcgaaa
     4801 ggcgtgatcg gcacggcgcg atcggcaagg cgtgatcgaa gagcgtatca gcgcccccgc
     4861 ccctcccccg cggccggcgg aggggccctg ttggaaggcc aacatgtatc gctcgtgcca
     4921 tcgtcgcctg caccttcacg cttctcgata acgcctcacc gataacgcct taccgatcgc
     4981 gcagcaccga taacgccttt ttcatgaaga cttctgcacc cgcaggtacc actcggacag
     5041 cagccgcacc tgcttctcgg tgtagccctt cgacaccatg cgcgcgacga agctctggtg
     5101 cttctcctgg tcctcggccg aggccttggc gttgaaggag atcaccggca gcaggtcttc
     5161 ggtgctggag aacatcttct tctcgatcac ctcgcgcagc ttctcgtagc tgctccacgc
     5221 cgggttcctg ccggcgttgt tggcgcgcgc gcgcaagacg aaattgacca cctcgttgcg
     5281 gaaatccttc gggttggcga tgccggccgg cttctcgatc ttctccaact cgttgttgag
     5341 cgccgcgcgg tcgaagatct cgccggtgtc cgggtcgcgg tactcctcgt cctgcagcca
     5401 gcagtcggcg taggtcacgt accggtcgaa gatgttctgc ccgtactccg agtaggactc
     5461 caggtaggcg gtctggatct ccttgccgat gaactcggcg tagcgcgggc tcagccaccc
     5521 cttgatgaac tccaggtact tgcgcttctg ttcctcgggg aagtcctcgc ggccgatgcg
     5581 ctgctcgagg acgtacatca gatgcaccgg gttcgcggcc acctcggtct ggtcgtagtt
     5641 gaacaccgcc gacagcacct tgaaggcgaa gcgggtggaa atgccgctca tgccctcgtc
     5701 cgggcccgcg tagtcgcgat actcctggaa cgacttggcc ttcggatcgg tgtccttcag
     5761 gctctcgccg tcgtagacgc gcatcttcga atacaggctg gaattctccg gctccttcag
     5821 gcgcgacagc accgagaact gggccatcat gtccagcgtc ccgggcacgc acggcgcatg
     5881 agcaagcgcg ctgtggcgca gcagcttctc gtatatcttg acctcttccg acacctgcag
     5941 gcaatagggc accttcacga tgtagatacg gtcgaggaac gcctcgttgg tgcggttgtt
     6001 gcggaagctc tgccactccg actcgttgga atgcgccagg atgatgccgt tgaacgggat
     6061 cgcggagaaa ccttcagtgc ctttgaagtt gccttcctgg gtggcggtca gcagcgggtg
     6121 cagcatcttg atcggcgcct tgaacatctc gacgaactcg aggaaaccct ggttcgccag
     6181 gcacagcccg ccggagtaac tgtaagcgtc cgggtcgttc tgagcgaagc ggtcgagctt
     6241 gcggatgtcc accttgccca ccagtgtcga gatgtcctgg ttgttctcgt cgccgggctc
     6301 cgtcttggcg atcgccacct ggcgcagcac cgagggctgc acgcgcacca cgcggaactg
     6361 accgatgtcg ccgtcgaact ccttcaggcg cttgaccgcc cacggcgaca tgaccccggt
     6421 gaggtagcgg tgcgggatcc tatactcctt ctccagcgtg tccccgtagc gctccggggc
     6481 gaacagcgcg agcggcgact cgttcaccgg ggagcccttc agcgcataga tcgggagttt
     6541 ctccatcagc tgcttcaggc gctcggcgat ggaggacttg cccccgccca ccgggccgag
     6601 caggtagagg atctgtttgc gctcctccag gccctgcgcg gcgtgcttga agaaggacac
     6661 gatctgcgag atcgcgtcct ccaagccgta gaactcggcg aaggccggat agcgcttgat
     6721 gaggcggttg gcgaaaatgc gcgacagccg cgggtcgtta cgggtatcga ccagatcggg
     6781 ctccccgatc gcctgcagga tgcgctctgc cgcgctggcg taggcgcccg gatcgcggcg
     6841 gcacagatcg agatactgtt ccagggtcat ctcctcgtcc tgcgcggccg agtaggtggc
     6901 cttgtagccc tcgatgatgc tggtcatgcg acatctccta gagttgatgc atcgcctcac
     6961 cttccattgt tgttgttgtg tgcgtccgcg tctgggcgtc tccacccctt cggtcagcac
     7021 gccgcgtgcc ggcctgcgag cggtccaact tgcacacccc gttgcccgtc gccaaactaa
     7081 ctcaaagctc agcgcccatg ttgcgagcag agcaagcgcc cgagtgccag cgatctgacc
     7141 ggcgctttgc gcgcttgcgc gagccgttca gcctgcgctt ttaacgcacg caacgcggtt
     7201 cggttacatc acgcgtgaag ctatcgcagc tgctttgtat tgcgcgctgc accgcctgcg
     7261 gcaagcgtgg ccgcagcggc ctcgaatcgg acggtaagca gcttccttgc caatggcgcc
     7321 tttgcgcctg aacatgcaag gaaactgatg gctcgcgctc cagtcgtcgc ctgtgcgccg
     7381 ccccccggcg gcggcaattc ggccgcatgg ccggagtttg cgagagcacc gcaccggcgc
     7441 ggattccggc gcaatgcgtc aatcgcgctg aagggtttag ggggaccctt tgccgcatcc
     7501 aaaccgtgcg cacgcctcac agccgtgcct ttgcgtcggg cccacgctgc attaggccgc
     7561 ttgcagcacc ccgggttcaa cccgctgctc ggtttcgagt tcgagtgccg cgggccgccg
     7621 atcggatcag gggattcaag tcgggctgca ctgcagccga taagacttga acgcgccgaa
     7681 tccgcgggcc cgaactttgc cagcacctct cgaatcgcct cgacattgga catccaagca
     7741 tgaaaaccac ccgtgttgct gtcgccgcgg ccctcctgct ggcaagcgcc ctgctgccgg
     7801 tgagcactga gctgcgcgcc taccagatca tcgagggacg ttaccgtgtc gaagtctcgc
     7861 cgggcgtatt cgaggatcag ctggtggtgc gctgcgacga cgggcgcacg ctcaccgtgc
     7921 gctgggaaac caagctcgcc gaggcctgtg gcgaaagcct gatgggcgga ccggcgccca
     7981 aggcggcacg cgcggccgcg cccagcccgg caccgaaggc ggcccaatcg ccgcaggccg
     8041 ccccgcagac gaagaccgcg cccgaaccgc cacccgcggc cgccacgccg gcgggcttgc
     8101 tcgacgagca gtctcagaag gatgcgatgc tggcgcaggt gcgtgcccag ttcggggacg
     8161 tgccggagca ctacgtcgag ttcaagcccg gccccgacgg tctgtccatg cgcttcctgc
     8221 cgccgctgtc cgacatcctc aagaagtacg agatgtgccg caaggtgcgc gagacgcgcg
     8281 ccgactgtgc cgccgaccgc gacaacgcga tcgccaaact gtccgcaacg cggccgccgg
     8341 cagccgcagg cactgacaaa gcggtggaca aagccaatcc ggccgtggca ggcgatgaca
     8401 agtcgctcgc gcctccagcc aaggccgcgg ctccaagcga gacgccggcg ccggcggccg
     8461 cgagcccgca ggcgcacgcc gccccggctc agccggatgc ggcagccgcc gctgcggcgg
     8521 atcgcgtcgc tgcagagcag aagatcctgg aggaatacac ctggtgcatg cgcgccaagc
     8581 cgaagttcga atgcgagcag gcgcgcgcaa aggcgctgag cgcgctggac aagccgaaac
     8641 ccgcggcgaa gcccaagcgc gcgcccaagc aggccgccgc gcccaacgtc gccgccaccg
     8701 cgcgctaacc cgtcctgttc cctcgccggc agggtgcgta caccttgtcc cttgacgctg
     8761 agatagcgcc tcgcgctcac accgccccgg gtagacggtt tttgacacat ctggacgctg
     8821 gccggcgtca gatctcgccg gccgcgcgcc gggccgccgc ggcgaggcac aggctgctgc
     8881 actaccatag cgcgccatgc ctcgcgcacg cctcgctccg ttcgcatcgc ccctgttcgc
     8941 agcgctcctg ttcgcagcgc ctctgttcgc agcactgggc ctcgcagcgc cgctgagcgc
     9001 tgccgcggcg agcttcgccc tgccctcgag cggagacgat ctggtgggcg agctgagcgt
     9061 ggtggtcgcg cgcgaggagg acaccctgct cgatatcgcc cggcagcacg acctgggctt
     9121 caacgagatc gccgccgcca accccgacat cgatccgtgg ctgccgggcg cgggcgcgcg
     9181 cgtggtgttg ccattgcagt tcgtgctgcc cgctggcccg cgcagcggca tcgtgatcaa
     9241 cctcgagcag atgcggctgt tctactttcc ggagagcggc gcgaatgccg gccgcgtcgt
     9301 cacccatccg ctgggcgtcg gtgtggagca tggcgccacg cccttggggc aaacgcgcgt
     9361 gatccgcaag gcggtcgatc ccgtctggcg cccgccgcaa tcgatccgcg ccgcgcgcgc
     9421 ggccgccggt gagaccctgc ccgaggtggt gccgcccggc ccggacaatc ccctggggcg
     9481 ccacgcgctg tatctcggct tccccggtta cctggtgcac ggcacgaacc gcccctgggg
     9541 gatcggcatg cgcgtgagcg gcggctgcat ccgcctgtat cccgaggaca tcgagtcgct
     9601 ctacgcgcag gtgccgcccg gcactgcggt gcgcatcgtg agcgagcctt tcgcagtggg
     9661 cctgctgcgg ggcatgccct acgtgcaggt gttcgcgcgg cccgagcgcg acgcggataa
     9721 ccgcagctac acgccgctgg tcgaggggct gatccgggcg gcgccgaacg ccgcgctgga
     9781 ctggggtcgg gtgctgcgca ccgccaagga gcagcgcgca ctcgctatcc ccgtaggccg
     9841 aagtgcgccg accctggagc agctcatttc cggggcgcag cgcgcggcgg cagcgccgcc
     9901 gcaggaacgc tgaggccggt gacagatgcg gaccaggcgt tgcgcggggt agcgcgcgcc
     9961 gacgcgcggt cacttgcgca tcgacttttc gaacatgcgg tcggtcttga tgtccaggcg
    10021 ctcgactgcg tcctgcgcct ccagggcagt gcgcttggcg tcctccgcag cggcctgggc
    10081 ggcacgggag gcctgcagca cctcctgcga catgtcgccg ctcacgggca tggcgcgcac
    10141 ggtggtgagg atctgatcgg ctttggcatt cgccgcctcg gcggcggcgc gcgccgcccg
    10201 ggcttcgttc agcgcgcttt gcgccgtggc cagcgcctgc tccacccggt cgggccccgc
    10261 ggcggtctgg gcgcctgccg cgccggcgcc gccggtggtg ccgcagcctt gggcaagcgc
    10321 gaacggcagt gcgagcagcg acatcctggt gatcgtcgaa cggcgattca cggctgatct
    10381 cctgttgttg gctgcattgg atcgaggcag gcatatcgcc ccgatgcggg gcgacgccgg
    10441 attataggcg gattcatcgg cagacaccgt cgggggcaga taccgccggg cgagacgatg
    10501 cggccggtac aattgtggcc gcccgcgcca ccgaccgttc ccaccccatg tccgcgttct
    10561 cgagcgcgcg ccgccccgtc gacgccaccg gggcggcgct gatgaccctc ttgtgcctgc
    10621 tgtgggggtt cacctacgtc ttctcgaagc tcgccgcggc cgacatctcg ctggtgctgc
    10681 agggcgggtt gcgctcggtg atcgcggcgc tgctgctgct cgtctgggcg cgcgcgcgcg
    10741 gcataccgct attcagccgc gacggcacgc tgggtgcggg gctggccgcc ggcgtgctgt
    10801 tcgcggccga gttcctgttc atctttgccg ggctggcgca caccggggcc tcgcgcatgg
    10861 tggtgttcat ctatctcgcc ccgtgcttca ccgcgctcgg cctgcagtgg ctggtccccg
    10921 gcgagcgctt gcacgcgcgc cagtgggccg gaatcgcgct cgccttcgtg ggcgtggccg
    10981 cggccttcgc cgacggcttc gcgtcggagc gcgcttcgct gctgggcgac gccttcggcg
    11041 tgatcgccgc cgcgttgtgg gcggcgacca ccgtgatcat ccggagcacc cggctgaaca
    11101 atgcgaacgc cgcgaagacc ttgttctacc agctggcggt cgccggggtg gccctgccgc
    11161 cggcctcgct gctgctcggc gagcccggcg tcgtggcgct cacgccgatc gccctggtga
    11221 gcctgttgta tcagggcgcg gtgatctcgt tcgcgagcta cctgatttgg ttctggttgc
    11281 tcactcgcta cctcgccgcg cggctgtcgg tgtttacgtt cctgtcaccc ctgttcggcg
    11341 tgaccgccag cgtggcagtg ctgtccgagc cactgcggcc cgccttcgtc gccgccgtgg
    11401 cgctggtggg cgccggcatc tacctggtca accggccggc gccagcgcat gcgctgcgat
    11461 cacctccgcc acgcaggccg tgagcttgcg ggcgtagggg atgtgcagga actcgttggg
    11521 gccgtgcgcg ttcgaatgcg gcccgagcac gccggtgatg aggaactgcg cgcccgggaa
    11581 aaccctgccc agcatgccca tgaagggaat ggtgccgccc tcgcccatcg ccgcggcagg
    11641 ccggccgtag tgcgcgcgcg acgcgccctg aagcgcgcgc tcgagccagg gcgcagtggc
    11701 gggcgcgcac cagccggtgg ccgcctggtc gggttcgaag cgcacgcgtg ccccgtgcgg
    11761 gggatcggct tcgagcagct cgcgcatgag cgccgtggcg cgctgcgcat ccaccggcgg
    11821 cggcagccgc agcgacagct tgagcgcggt ccccggccgc agcacattgc ccgcgtcgcg
    11881 gatcgccggc atgccatcgg cgccgaccac cgacaggaac ggccgccagg tgcgattcag
    11941 gatcgcctgc gtgaagtccg tggtcagcgg ctgcgtaccg ccggcaaacg ggaacttggc
    12001 gtacacggac tcgcgcagga tctcgccggc ttgccgtgcc tgttccacgc gctcgggcgg
    12061 gatctggcag tgcagctcgg gcggccggac gcgcccggtg gccgactctt ccagccggtc
    12121 gagcagcatg cgcgcaacgc gaaacgacga aggcaccacc ccgctcgcat cgcccgagtg
    12181 cacgccttcg gccaggacct cgacgccgag cgttccggcc gcgagcccgc gcaacgacgt
    12241 ggtcacccag agctgctcgt agttgccgca acccgagtcc aggccgatca ccagatccac
    12301 cttgcccagg cgcggcgtca gggcctcgag gtaggccggc agatcgtagc tgccgctctc
    12361 ttcgcagcac tcgatcagcc ccgccacccg tgcgtgcggg tggccctgcg cgcgcagcgc
    12421 cgtcagcgcg gccagcgcgg cgaacaccgc gtaaccgtcg tccgccccgc cgcgcccgta
    12481 gagcctgtcg tcctcgacca cggggctcca gggtccgagc cctgcgcgcc agccgaccat
    12541 ctccggctgc ttgtcgagat gtccgtagat cagcacggtg ccttgcgccc ccgcacccga
    12601 cgcgggaacg tccaagtaga gcaccggcgt gcgcccgggc agccgcagga tctcgagctg
    12661 catgccgggg acctgctggg cgctgcacca ccggtgcgcc aggccgatcg cggcttcgat
    12721 gtggccgttg ccctgccact gcgcatcgaa atgcggcgac ttgcagggga tacggatgta
    12781 ttcgaccagg cgtgggacga tgtcctcgtt ccacttgcga tcgacgaagg cggacagcgc
    12841 gcgcgagtcc agggcgggca tcggggtggt gcgaaacggg ggcgacggcg cgagtgtacc
    12901 ccggcgcgcc gccggggctc taggggcgca accgcctcac gcctcgcgca gagcgaagcg
    12961 cgcgatctcg aagtcgctgc cgctgtcggc gatgcggcgg cgctcgatgc gatacaggcg
    13021 gcagtgcatg cgcagctcgc agctcgcgag ccgcgccgtc gaaccgcgcg gcaagaccag
    13081 ctcgacctcg ccgccggcca ggctggtctg ggcctcggtg ggcagcagga tgccgtgcag
    13141 cggctgcaga ccctccttga gcgggagcgg cgccagctcg acgcatacgc cgccgcgggc
    13201 caggatctcc acgccgaccg atcgatgcat ggggctgcgc gcggtgaggc ggcggatcac
    13261 gcccacacac cacgacgagg gccagtccgg cttggccgcc accaggcgtc cgacctccag
    13321 ccagtcgtcg tcgcgcgccg ggatcagcgc gccgaagccg ccctcgctct cgttttcgac
    13381 ggtccaggcc tcggtcactt cgtcctgcgc ctgctccccg ccgacggcgg ccagcacgcg
    13441 ctcgaagccg tgcgccacgt gcgcggtggc gagcacgcgg tggcgcgcct cgccgcgcgt
    13501 cggcggcttg gcgccccagt ggcgcgccag gtgctgcagc acctcgcgca ccagggacgg
    13561 ctcgaactcg ccgccgagcc gcagctgcgc cggcacgcca cgctgtgcgg cgagctgggc
    13621 gaggaaaccc tcgaccatca cgccggcgtc gccgggaccg aagaagcgca caccgcgcgc
    13681 cggcgcgatg ccctcgccga tgcgataggg cgggcggctc gccttcagat ccacggcaaa
    13741 gtggcagccg gcggccggcc ggcggtgcag caggaagaat tcggcgaccg cggcgatcac
    13801 ccgctcggcc accgcctggg cggccggcag caggttgccc atggccgaca ccgacagcac
    13861 cagcgcccgc aggtattcgc ggcgcacggt ggaatcgccg ctcatcgccg ggtagatctt
    13921 gaagcgcacc gtggcgagcc cggcgcggtc gcacaatgcg tacaactgtc ccagccgcgg
    13981 ccagatctcg tcgtccaccg cgctgtagcg cagcagcgcc cagcgcagtt ccagcgtgtg
    14041 cgcgcgcacc gcgcgcgcgg ccaggaccgg caccagcttg cgctcgccct ggccctggga
    14101 caggcgcagg cacgcgctgt agccgtcggc cagatggcgc gcatagcgcg ccgccgcctg
    14161 ccacaggcgc tgctcgcgat aggtctgcat gcgcccgccg gactccagat actcgtgcag
    14221 cagcgccagc cggtgcggct gcaccagcgc atccaggcgg tcgatcacct cgaatatgcg
    14281 caacgggtcg gacgacgggt tctggccgta ggcctccagc cagaaagccg cctcctccat
    14341 cgcgcccagc ggatcggccg tgggcaggac gatgggcagc ggcggcgcct tggggacggc
    14401 gccctccggc tggcgggtgg cggacgaagc gcttttcacg gtggcagcag gtcgaaccca
    14461 gtcgcccgca ggcaagctct gcgccaggcg gctccccggt ggcggccagc cgcggtgcat
    14521 tcgggcgtgg cctgtcgctt gctttgcgga gcatgccggc catgagagaa tcgcgctccc
    14581 acgtcaccat cgccggctcg atgtccgccg tcaccctcac cgcccgcccc gccgtcttgc
    14641 agctgcgggc ttcgcgcatc cgcgaagtgg ccaatgccgc catggggcgg gaggacattc
    14701 tcgcgttctg gttcggcgag ccggacgagg tgacgccgga attcatccgc cgcgccgccg
    14761 tagagtcgct cgagcgcggc gagacgttct acagccagaa cctcgggctg ccggagctgc
    14821 gccagacgct ggcgacttac atatcgcgcc tgcactgccc caccacagcc gaccagatcg
    14881 cggtcaccag ctcggggctg tcggcgttgc agctcgccag tcaggcgctt gcgggccacg
    14941 gcgagcgcgt ggtcgaagta acgccgctgt ggcccaacct ggtggaaatc ccgaagatcc
    15001 tcggcgcgca ggtggagtgc gtaccgctgc gcttcgaccc gggcggctgg cgccttgatc
    15061 tggaccgcct gctcgacgcg ctgaccccgc ggacccgggt gctgttcgtc aactcgccga
    15121 acaatcccac cggctggacc gtcggccggg ccgagcagcg cgcgatcctg gagcactgcc
    15181 gccggcacgg catctggctg gtagccgacg acgcctacga gcgcctgtgt tacgacgacg
    15241 acgccggcgg cggcgtggcg ccgtccttcc tcgacctggc ggacccggga gaccgcgtgg
    15301 tcagcaccaa caccttctcc aagtcctggt tgatgaccgg ctggcgcctg ggctggatgc
    15361 gcgggccggc ggcgctgatc gcggaaatgc ccaagctgat cgagtacaac acctcatgca
    15421 cgccggtgtt cgttcagcgc gccggcgtgg tcgcggtaag cgagggcgag ccggtggtag
    15481 cgcacacgtt gacgcgcttg cggcaggcgc gcgacttctt gctggagcag ctcgcgcgca
    15541 ccccgggcat cgaggtggca cggccggcgg gcgcgatgta tgcgttcttc cgcgtggccg
    15601 gggtgcacga cagtctggcg ctgtgcaagc atctggtcgc cggcg
//
LOCUS       DASPGE010000084         3402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1198298, whole genome shotgun sequence.
ACCESSION   DASPGE010000084 DASPGE010000000
VERSION     DASPGE010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198298"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VD791_02655"
     CDS             complement(<1..428)
                     /locus_tag="VD791_02655"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HXV06903.1"
                     /translation="MKPTAIALAAALAAAAPVALAGGGSFQDYARVKDVDPEYERVNV
                     PRKECYSEYVPENSYQRNGGKRLVGPLIGGVAGGLLGSQVGKGNGKVAAAAAGAAIGA
                     IVGDRLSERRAGAEYYEREVRRCRMVDNWETRIVGYRVTY"
     gene            complement(468..818)
                     /locus_tag="VD791_02660"
     CDS             complement(468..818)
                     /locus_tag="VD791_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06904.1"
                     /translation="MKSRLLTAALGGLLLLGSGLALADDGGRGRYDRGGNHGWHDRGS
                     ANGPRWSPPRHYRGHGHGHFKPHWKQGHRFGHHRDYGLRDRHRYHRGHQGHHWKRYAD
                     RGDDITIIFRGSLD"
     gene            946..1770
                     /locus_tag="VD791_02665"
     CDS             946..1770
                     /locus_tag="VD791_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV06905.1"
                     /translation="MDNAPAPPADYIAEIEDVHFAYGERQVLSGVSLRIPRAKVVAIL
                     GSSGSGKTTLLRLLGGQARPDRGRVSVAGQVVHELDTRRLYELRRHMGMMFQAGGLFS
                     DLSVFDNIAFPLREHTRLSEDIVRDLVLMKLHAVGLRGAQGLMPAELSGGMSRRVALA
                     RAIAMDPMLIMYDEPFAGLDPISLNVIANLIRRLNDALGATSIVVTYDVSESLKVADY
                     AFFLSDGQIAAHGAAAQMSASRDPFVHQFVHAEPDGPVAFHYPAGALQSDFDFPSI"
     gene            complement(1808..2398)
                     /locus_tag="VD791_02670"
     CDS             complement(1808..2398)
                     /locus_tag="VD791_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HXV06906.1"
                     /translation="MSAHCDTLAATALRALPRRFYERDTVAVARELLGTYLAHVQQGV
                     ERVGRIVEVEAYLGPHDLAAHSSKGLTARNRSMFGPAGHAYVYMIYGVHFCMNVVTGG
                     AGQGSAVLLRALEPVRNVTARTRGPGLLCKAMHIDRRLDGHDLLSATLFIAAAQPPGA
                     RARIACARRIGVDYAGPWSRRLLRFYLKGNPFVSRS"
     gene            2504..3166
                     /locus_tag="VD791_02675"
     CDS             2504..3166
                     /locus_tag="VD791_02675"
                     /inference="COORDINATES: protein motif:HMM:NF018755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HXV06907.1"
                     /translation="MLAGVGVAAWSVAAETPGFAGRVVVEWLHDDPFIARMRLVEDFA
                     FRDPAGRHWVAEKGHVLDGRSIPLLFRDLVGPPFEGEYRRSAVVYEVQAHRMQEPWRA
                     VHRLFYHASRAEGVPDADAKLMYLALYAGGLRWEQPGSTCFNHCHTAAQSLSWMPATT
                     PAEMRPLVEWIRQVDPGLDQIDRRVDATLRKPGPHIFGQGFTSPILADPETAPSQSPE
                     KE"
     gene            3169..>3402
                     /locus_tag="VD791_02680"
     CDS             3169..>3402
                     /locus_tag="VD791_02680"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV06908.1"
                     /translation="MFVKRAGERQWMSAGYEGAERALLRLSKEEGRTSIVRLKAGSRG
                     PRHTHAAGEDVLVLSGKVRLAGEILAAGDYMYTE"
BASE COUNT          515 a         1158 c         1188 g          541 t
ORIGIN      
        1 tcgtaggtca cgcggtaacc gacgatgcgg gtttcccagt tgtccaccat gcggcagcgg
       61 cgcacttcgc gttcatagta ctccgcgccg gcgcggcgct cggacagccg gtcgccgacg
      121 atggcgccga tggccgcgcc ggcagccgcc gcggccacct tgccgttgcc cttgcccacc
      181 tgcgagccga gcaggccgcc ggcgaccccg ccgatcagcg gtccgaccag gcgcttaccg
      241 ccgtttcgct ggtagctgtt ttccggcacg tactcggagt agcactcctt gcgcgggaca
      301 ttcacccgct cgtattccgg gtcgacgtcc ttgacccgcg cgtaatcctg aaagctcccg
      361 ccgccggcga gggcgaccgg agcggcggcg gcaagcgcgg ccgccagcgc gattgcagtg
      421 ggcttcatgg catgctcctt cgatccggtt ggtcgctgcg cagccgctca gtccaggctg
      481 ccgcggaaga tgatggtgat gtcgtcgccg cggtcggcgt agcgcttcca gtggtgaccc
      541 tgatggccac gatgatagcg gtgccgatcg cgcaagccgt agtcgcggtg gtggccgaag
      601 cggtgtccct gcttccaatg gggcttgaag tgcccgtggc catgcccgcg gtaatgccgg
      661 ggagggctcc agcgcggtcc gttggccgac ccgcggtcat gccagccgtg atttccgccg
      721 cggtcgtacc gcccgcgtcc gccgtcgtcg gccagcgcca ggccactgcc cagcagcagc
      781 agtccgccca gtgcggcggt cagaagtctc gatttcattt ggcaatctcc ttgcaaaagt
      841 ccaggacgcc cctggttgag cgcagattac gcaaggggcg ctgaacggaa gctgaacgaa
      901 gaggcggccg gccgggctac aatcgcgcct ggaatccgcc aacccatgga caacgccccc
      961 gccccgccag ccgactacat cgccgagatc gaggacgtgc acttcgccta cggcgagcgc
     1021 caggtcctga gcggcgtaag tctgcgcatc ccgcgcgcca aggtggtggc gatcctcggc
     1081 agcagcggca gcggcaagac cactttattg cgcctgctcg gcggtcaggc gcggcccgac
     1141 cgcgggcggg tgagcgtggc cggacaggtg gtgcacgagc tcgacacgcg gcgcctgtac
     1201 gagctgcgcc ggcacatggg catgatgttc caggcgggcg gcctgttcag cgacctgtcc
     1261 gtgttcgaca acatcgcctt cccgttgcgc gagcacacgc ggctctcgga ggacatcgtc
     1321 cgcgacctgg tgctgatgaa gctgcacgcg gtgggtctgc gcggcgccca gggtctgatg
     1381 ccggccgagc tgtcgggcgg catgtcgcga cgcgtcgcgc tggcgcgcgc catcgccatg
     1441 gacccgatgc tgatcatgta cgacgagccg ttcgcgggcc tggacccgat ctcgctcaac
     1501 gtgatcgcca acctgatccg gcgtttgaac gatgcgctgg gcgccacttc gatcgtggtc
     1561 acctacgacg tgtcggagtc gctgaaggtc gccgactacg ccttcttcct ctcggacggc
     1621 cagatcgccg cgcacggggc cgcggcgcag atgagcgctt cgcgcgatcc cttcgtgcac
     1681 cagttcgtgc acgccgaacc cgacggtccg gtggcgtttc actatcccgc gggcgcgctg
     1741 cagtcggact tcgatttccc atcgatatag cccggatgca gcgaagcgga atccggggaa
     1801 atccagctta gcttcgcgaa acgaacgggt tgcctttgag gtagaagcgc agcagccgcc
     1861 ggctccaggg ccccgcgtag tccaccccga tgcgcctggc gcaggcgatg cgcgcgcggg
     1921 cgcccggcgg ttgcgccgcg gcaatgaaca aggtggcgct cagcaggtcg tgcccgtcca
     1981 ggcgccggtc gatgtgcatg gctttgcaca gcagcccggg accccgcgtg cgcgcggtga
     2041 cgttgcgcac cggctcgagc gcgcgcagca gcaccgccga gccctgacct gcaccgccgg
     2101 tcaccacgtt catgcagaaa tgcacgccgt agatcatata gacataggcg tggccggccg
     2161 gcccgaacat cgaccggttg cgcgcggtaa ggcccttcga ggagtgcgcg gcgaggtcgt
     2221 gcggccccag gtaggcttcg acctcgacga tccggccgac ccgctcgacg ccttgttgca
     2281 cgtgggccag gtaagtgccc agcagctccc tggccacagc aacggtgtcg cgctcgtaga
     2341 agcggcgcgg cagcgcgcgc agcgcggtgg cggccagggt atcgcagtgg gcgctcatgg
     2401 cgccgcagct tagcacctgc gccggtggac gcccgcgcga agacgtgttt tcataggcga
     2461 gaacctatcc ccgggggcgt gggaatttaa gcgactgtac gccgtgctgg ccggcgtggg
     2521 cgttgccgcg tggtccgtgg ccgccgagac gccgggtttt gccggccgag tcgtggtcga
     2581 atggctgcac gacgatccgt tcatcgcccg catgaggctg gtggaggact tcgccttccg
     2641 cgacccggcc ggccggcact gggtcgccga gaaagggcat gtgctcgacg ggcgctcgat
     2701 cccgctgctg tttcgcgacc tcgtcggccc gccgttcgag ggcgagtacc gcagatcggc
     2761 ggtggtgtac gaggtgcagg cgcaccgcat gcaggagccc tggcgcgcgg tgcaccgcct
     2821 gttctaccac gccagccgcg cggagggcgt gcccgacgcc gacgccaagc tcatgtacct
     2881 ggccttgtat gcgggcggct tgcgctggga acagcccggc tcgacctgtt tcaaccattg
     2941 ccacacggcg gcgcaatcgc tgtcgtggat gccggcgacc acgcccgctg aaatgcggcc
     3001 gctcgtcgag tggatccggc aggtcgaccc ggggctcgat cagatcgacc gccgcgtcga
     3061 cgccacgctg cgcaagccgg gcccgcacat cttcgggcag gggttcacca gtccgatcct
     3121 cgccgacccc gagaccgcac cgtcgcaatc accagagaag gagtgagcat gttcgtgaag
     3181 cgtgcaggag agcgccagtg gatgtccgcg ggctacgagg gggccgagcg cgcgctgctc
     3241 aggctcagca aggaagaggg gcgcacctcg atcgtgcgcc tgaaggccgg ttcgcgcggc
     3301 ccgcgccaca cgcacgcggc cggcgaggac gtgctggtgc tgtcgggaaa ggtgcggctg
     3361 gccggtgaga tcctcgccgc tggcgactac atgtacaccg ag
//
LOCUS       DASPGE010000085         6585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_378264, whole genome shotgun sequence.
ACCESSION   DASPGE010000085 DASPGE010000000
VERSION     DASPGE010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6585
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378264"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VD791_02685"
     CDS             complement(<1..784)
                     /locus_tag="VD791_02685"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HXV06909.1"
                     /translation="MPEPATAKTRIFHELDFDKPGKQIGYLRVPQSRDGGAWSTIEIP
                     IAVINGSKGRGVLFTGGVHGDEYEAQIAISRLARRIDPQSVRGRIIMLPAVDLPAALA
                     GRRMCPIDGRDFNRCLPGDPRGSFCQMLAHFIDSAILPQVEVLVDVHAAGNSMEAALS
                     TTMHYVDDAAIVRKTRELAESFAAPYNVMFWGVDEGGTMAAAAERNGVLAISSELGGY
                     GRVSVEGVRVAQRGLDNVLKWMGMIEGTPDTSQRDGSGGTRHM"
     gene            complement(837..2084)
                     /locus_tag="VD791_02690"
     CDS             complement(837..2084)
                     /locus_tag="VD791_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV06910.1"
                     /translation="MTRILFTNCKVLDCTGASPFSGSVLVEGERIHEVWRAQQTPRLE
                     DARAIDGKGATLMPGLIEPHAHITFTDCAQSVDMGFIPVEEHLLIALRNARTMLDAGF
                     TSCLSAASAKKRLDIVLRNAIDRGEFPGPRMLAASPEMTVTGGLGDVRLPHLYRENFA
                     VVLDGADEFRRFAREMCRDGVDTLKINISGDAGTPAAPADRTVMSEAEIHAVCDVAHD
                     FGKRVAAHARSAESVKRCLHFGVPILYHATLLDQEAKDMLSAARDTVFVAPTLGNLYA
                     TLYEAGAWGMSVEAGRARGLEHELEQGIATMKDLRRRGVRILPGGDYGFAWNPIGANA
                     RDVEHFVKLLGFTPLEAILAATRLGGEIMQRGHELGQVRPGYLADLLLLDGDPLADVG
                     VLRQRERLRMVMKGGAIHARCEP"
     gene            complement(2124..2885)
                     /locus_tag="VD791_02695"
     CDS             complement(2124..2885)
                     /locus_tag="VD791_02695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV06911.1"
                     /translation="MGLQGKVTWITGAGSGIGLAGVQALSEAGATVVMSGRRGEVLER
                     EAGKLLKAGRRVEVEPLDVSDREAVARVAQRVLSRHGKVDILVNSAGLNVVNRYWRNQ
                     TPDGWDEVIGINLDGSFYTTLAVLPAMRAQRDGLVINLSSWAGVYHAALTGAAYNASK
                     HAVIAMTETINMEECVHGIRACAICPAEVATPIMDRRPVPPSAEDRAKMLQPEDLGRT
                     IRFVAELPPHACINQIIISPTWNRIYVGGADPTRR"
     gene            complement(2935..3888)
                     /locus_tag="VD791_02700"
     CDS             complement(2935..3888)
                     /locus_tag="VD791_02700"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXV06912.1"
                     /translation="MPLDPQADFILGLVKTSGLPELWQLTPDQAREQFKLRVAKLAQK
                     ESIYRSYDRRIPGPGADLAIRIYAPREAKPGERFPVLLWFHGGGFVIGDLDTHDPACR
                     ALATQAECLVVAVDYRLAPECKFPAAVDDSMAALRWLALHAPEIGGDPARIAVGGDSA
                     GGNLAAVCSILARDDGYPKLAHQLLIYPCTAPEPETPSHHQFAEGHLLTRNTITWFYK
                     QYVRSPREFNDFRFAPLVAKDLSNLPPALVLVAGYDPLRDEGVDYAKRLIEAGNRVTL
                     VNYEGMIHGFYLMGGAVDAARRALSQSAQALKEAFVSSAAG"
     gene            complement(3908..4264)
                     /locus_tag="VD791_02705"
     CDS             complement(3908..4264)
                     /locus_tag="VD791_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06913.1"
                     /translation="MLVVAFVGLVWGGIWLRNAAPAVAPLLGQAIGPTGAMFITPIAL
                     AAAVGLWLRKSWGWWFSLVVAAYQTVSYLLFLMVVLASGDATGLLTWLTGLLLLALLV
                     VLLLPGTRRACIAPST"
     gene            complement(4342..4743)
                     /locus_tag="VD791_02710"
     CDS             complement(4342..4743)
                     /locus_tag="VD791_02710"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXV06914.1"
                     /translation="MPPRFDVLIPFLEHLGLRLLEIGGGRARVGFDPKPEHLNSWKAI
                     HGGGVMTVLDAALSSACRSLDEGCIGATTVELKVNFIAAARGPVIAEGRAQRAGRSLL
                     FAEGELFDREGAVLAKATGTFKLVYPQAPGE"
     gene            4807..5271
                     /locus_tag="VD791_02715"
     CDS             4807..5271
                     /locus_tag="VD791_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06915.1"
                     /translation="MARSSGRSPSSGKTPATAAERMRRYRARKRAAGLKQERRWVPAQ
                     GERLAVYSDHAVLDARSLAMHCLIARKISRDPQLLEVARRNLDAWKRARPAGERPRYL
                     AEWERVLSRPWPQIAAFITAGSGPAVRLRQSSPFAGVLDPMERRRVYEAFRA"
     gene            5276..5800
                     /locus_tag="VD791_02720"
     CDS             5276..5800
                     /locus_tag="VD791_02720"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HXV06916.1"
                     /translation="MIRAAGAIAADSQIVVIGSQAVLGQFPRAPVSLLASMEADVYPR
                     NKPELSDLVDGSIGEGSFFHERFGYYAQGVSPQTATLPAGWEKRLISVSNPNTGGVEG
                     LCLEVHDLAVSKYVAGREKDLEFTRELARHGMTQRAALLERLGQTRLSAAQRRVIQAR
                     IARDFPDPPAGRGA"
     gene            complement(5843..>6585)
                     /locus_tag="VD791_02725"
     CDS             complement(5843..>6585)
                     /locus_tag="VD791_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV06917.1"
                     /translation="VALITGGSRGLGLQIAEALGEMGARLVITARKQHELDEAAAHLQ
                     GQGAEVLTVAGDLQDPHAAKALVEKVLAERGRLDILVNNAGATWGAPAEEHPLDAWNK
                     VINLNLTAVFALTQAAATRAMIPARYGRIVNIASVAGLNGTHPDFMRTIAYNASKGGL
                     VNFTRSLAAEWAQYGITVNAIAPGVFPTKMAKVMAERHMDTIMQLTPMKRLGGDEDLK
                     GVAVLLASDASSYITGQIVAVDGGMLAI"
BASE COUNT          987 a         2285 c         2271 g         1042 t
ORIGIN      
        1 ccatgtgccg cgtgccgccg ctgccgtcgc gctgcgaggt gtccggcgtg ccttcgatca
       61 tgcccatcca cttgagcacg ttgtcgaggc cgcgctgcgc cacccgcacc ccctcgacgc
      121 tcacccgtcc gtagccgccg agctcgctcg agatcgcgag cacgccgttg cgttcggcgg
      181 ccgccgccat cgtaccgccc tcgtccacgc cccagaacat gacgttgtag ggtgcggcga
      241 aactctcggc cagttcccgg gtcttgcgca cgatcgccgc atcgtcgacg tagtgcatgg
      301 tcgtgctgag cgccgcctcc atcgagttcc ccgcggcatg cacgtcgacc aggacctcga
      361 cttgcggcag gatcgcgctg tcgatgaagt gcgccagcat ctggcagaag ctgccgcgcg
      421 gatcgccggg caggcagcga ttgaaatcgc gtccgtcgat ggggcacatc cgccgtccgg
      481 cgagggcggc gggaaggtcc acggccggca gcatgatgat ccggccgcgc acgctttgcg
      541 ggtcgatgcg gcgcgccagc cgcgagatgg cgatctgcgc ctcgtactcg tcgccgtgca
      601 cgccgccggt gaacaggacg ccgcggcctt tgcttccgtt gatcaccgcg atcggaatct
      661 cgatcgtcga ccaggcgccg ccgtcgcgcg actgcggcac gcgcagatag ccgatctgct
      721 tgccgggctt gtcgaaatcc agctcgtgga agatgcgggt cttggcggtt gcgggctcag
      781 gcatgctatg gctccccaga agaaaaagtt ccggacgcgg tgactggcga tcgcgctcaa
      841 ggttcgcacc tggcgtgtat cgcgccgccc ttcatgacca tgcgcagccg ctcccgctgg
      901 cggagaacgc cgacgtccgc tagcggatcg ccgtccagca gcagcagatc ggccaggtag
      961 ccggggcgca cctgccccag ctcgtggccg cgctgcatga tctcgccgcc gagcctggtg
     1021 gcggcgagga tcgcctccag cggcgtgaag ccgagcagtt tgacgaagtg ctcgacgtcg
     1081 cgcgcattcg ccccgatcgg attccaggcg aagccgtagt cgccgccggg caagatgcgc
     1141 acgccccgcc ggcgcaggtc tttcattgtt gcgatgccct gctcgagttc gtgctcgagc
     1201 ccgcgcgcgc gtcccgcctc gacgctcatg ccccacgcgc cggcctcgta cagggtcgcg
     1261 tacaggttgc ccagggtggg cgccacgaat accgtgtcgc gcgccgcgct gagcatgtcc
     1321 ttggcctcct ggtcgagaag ggtcgcgtgg tacagaatcg gcacgccgaa gtgcaggcag
     1381 cgcttcaccg actcggcgct gcgcgcatgg gcggccacgc gcttgccgaa gtcgtgcgcg
     1441 acatcgcaca cggcgtggat ctcggcctcg gacatgaccg tcctgtccgc cggcgcggcc
     1501 ggggtgccgg cgtcgccgga gatgttgatc ttgagcgtgt cgacgccgtc gcggcacatc
     1561 tcgcgcgcga agcggcggaa ttcgtcggcg ccgtccagga ccaccgcgaa gttctcccgg
     1621 tacaggtgcg gcaggcgcac gtcgcccagc ccgccggtga ccgtcatctc cggcgaagcc
     1681 gcgagcatgc gcgggccggg gaactcaccc cggtcgatgg cgttgcgcag cacgatgtcg
     1741 agccgcttct tcgccgaggc ggcactcagg cagctggtaa agcctgcgtc gagcatggtg
     1801 cgcgcgttgc gcagcgcgat cagcaggtgc tcctcgaccg ggatgaagcc catgtcgacc
     1861 gactgcgcgc agtcggtgaa cgtgatgtgc gcgtgcggct cgatcaggcc gggcatcagc
     1921 gtcgcgccct tgccgtcgat cgcccgcgca tcctccaggc gcggcgtctg ctgcgcacgc
     1981 catacctcgt ggatgcgctc gccctccacc agtacgcttc cggagaacgg cgaggcgccg
     2041 gtgcagtcga gcaccttgca gttggtgaac aggatacggg tcatcgcagc gccctccctg
     2101 cgttgcgacc ggcgctctcg gtttcagcgt cgcgtcgggt ccgccccgcc gacgtagatg
     2161 cggttccagg tggggctgat gatgatctgg ttgatgcagg cgtgcggcgg cagctcggcc
     2221 acgaagcgga tggtgcggcc caggtcctcg ggctggagca tcttcgcgcg atcctcggcg
     2281 gacggcggca ccgggcgccg gtccatgatc ggggtggcga cctcggccgg acagatcgcg
     2341 caggcgcgga tgccgtggac gcattcctcc atgttgatgg tctcggtcat ggcgatgacc
     2401 gcgtgcttgc tggcgttgta ggcggcaccg gtgagcgcgg cgtggtacac gcccgcccac
     2461 gaggacaggt tgatcaccag gccgtcgcgc tgcgcgcgca tcgccggcag gaccgcgagc
     2521 gtggtgtaga acgatccgtc caggttgatg ccgataacct cgtcccagcc gtccggcgtc
     2581 tggttgcgcc agtaccggtt gacgacgttg agcccggcgc tatttaccag gatgtccacc
     2641 ttgccgtggc gcgagagcac ccgctgggcg acgcgcgcga ccgcttcgcg gtcgctcacg
     2701 tcgagcggct ccacctccac acgcctcccg gccttcagga gcttgcccgc ttcgcgctcg
     2761 agcacctcgc cgcgccgccc cgacatcacc accgtcgcac ccgcctcgga cagcgcttgc
     2821 acgcccgcga ggccgatgcc gctgccggca ccggtgatcc aggtgacttt cccttgcaga
     2881 cccatcatcg agctcctctc tgccgcaccc attgcatgat agcgctgcgt cgagctagcc
     2941 cgctgcgctg gagacgaacg cttccttgag cgcctgcgcc gactgcgaca gcgcccgccg
     3001 cgccgcgtcc accgcgccgc ccatcagata gaagccgtgg atcatgccct cgtagttcac
     3061 cagcgtcacg cggttgccgg cctcgatcag ccgcttcgcg tagtccacac cctcgtcgcg
     3121 cagcggatcg tagccggcca ccagcaccag cgcgggcggc aggttggaga ggtctttcgc
     3181 gaccagcggg gcgaagcgga aatcgttgaa ctccctgggg ctgcgcacgt actgtttgta
     3241 gaaccaggtg atggtgttgc gcgtgagcag atggccctcc gcgaactggt gatgcgaggg
     3301 cgtctccggc tcgggcgccg tgcagggata gatcagcagc tggtgggcga gcttcggata
     3361 gccgtcgtcg cgtgcgagga tcgagcacac cgcggccagg ttgccgcctg cgctgtcgcc
     3421 gccgaccgca atgcgcgcag gatcgccgcc gatctcgggg gcgtgcagcg ccagccagcg
     3481 cagcgccgcc atgctgtcgt caaccgccgc cggaaacttg cactcggggg cgagccggta
     3541 atcgaccgcc accaccaggc attcggcctg cgtggccagc gcgcggcagg cggggtcgtg
     3601 cgtgtcgagg tcgccgatca cgaaaccgcc gccgtgaaac catagcagca ccgggaagcg
     3661 ctcgccgggc ttcgcctcgc gcggcgcgta gatgcgaatg gcgagatccg cgcccgggcc
     3721 cggaatacgc cggtcgtagc tgcggtagat cgactccttc tgggccagct tggccacgcg
     3781 cagcttgaac tgctcgcgcg cctggtccgg cgtgagctgc cacagctcgg gcagaccgga
     3841 ggtcttgacc agcccgagaa tgaagtcggc ttgcggatcg agaggcatgg ggagtttcct
     3901 gttgagatca agtcgacggc gcaatgcagg cgcggcgcgt gccgggcagc agcagcacga
     3961 ccagcaatgc gagcaggagc aggcccgtga gccaggtgag gagcccggtg gcgtcaccgc
     4021 tcgcgagcac gaccatcaga aacagcaggt acgacaccgt ctgataagcg gcgacgacga
     4081 ggctgaacca ccagccccag gacttgcgca gccacagccc gacggcggcg gccagcgcga
     4141 tgggcgtgat gaacatcgcg ccggtggggc cgatcgcctg accgagcagc ggcgcgacgg
     4201 ccggcgccgc gttgcgcagc cagattccgc cccacaccag cccaacgaat gcgacgacga
     4261 gcatcgcggc gactcgcggc agagccgggc cgccgggggc ggaaggcgcc tgcacggcgc
     4321 cgtgcatggc gctctgaacc gtcactcgcc gggcgcctgc ggatacacca gcttgaaggt
     4381 gccggtggcc ttggccagca ccgcgccttc tcgatcgaac agctcgccct ccgcgaacag
     4441 cagcgaccgg cccgcacgct gcgcccggcc ctcggcgatc accggtccgc gcgcggcggc
     4501 gatgaagttg accttcaatt cgacggtggt ggcgccgatg cagccctcgt ctaatgagcg
     4561 gcaggcgctg ctcagcgccg catccaggac ggtcatcaca ccgccgccgt ggatcgcctt
     4621 ccagctgttc aggtgctcgg gcttgggatc gaaccccacg cgcgcgcggc ctccgccgat
     4681 ctccagcagg cgcaggccga ggtgctcgag gaaggggatc agtacgtcga agcgaggcgg
     4741 catgggcgaa ttctacctgc tcggacggaa cttgaggcga tcttcgtaac gcgttacact
     4801 gccggcatgg cacgctcatc cggcagatcg ccctcatcgg gcaaaacgcc cgccaccgct
     4861 gcggagcgca tgcggcgcta ccgcgcccgc aaacgcgcgg ccgggctcaa gcaggagcgt
     4921 cgctgggtcc cggcgcaagg cgagcggctt gccgtctatt ccgatcacgc cgtgctcgat
     4981 gcgcgcagcc tcgccatgca ctgtctgatc gcccgcaaga tcagccgcga cccgcagctg
     5041 ctcgaggtgg cgcggcgcaa cctcgacgcc tggaagcgcg cccgccccgc gggcgagcgg
     5101 ccccgctatc tggcggagtg ggagcgcgtg ctgagccgcc cctggccgca gatcgccgcg
     5161 ttcatcaccg ccggttcagg tccggcggtg aggcttcggc agtcctctcc ctttgcggga
     5221 gtgctcgatc caatggaaag gaggcgcgtc tacgaagcgt tccgagcttg agcacgtgat
     5281 ccgcgccgcc ggggcgatcg ccgccgacag ccagattgtg gtgatcggca gtcaggcggt
     5341 gctggggcag tttccgcgcg cgccggtcag cctgcttgcc tcgatggaag ccgacgtcta
     5401 tccgcgcaac aagccggagc tgtccgatct ggtcgacggc tcgatcggcg aaggctcgtt
     5461 cttccatgag cgcttcggct attacgccca gggcgtgagc ccgcagaccg ctaccttgcc
     5521 cgccggctgg gagaagcggt tgatctcggt cagcaatccg aacacagggg gcgtggaagg
     5581 gctgtgcctg gaggtgcacg atctggcagt ttcgaagtac gttgccggac gcgagaagga
     5641 tctggagttc acgcgggaac tcgcgcgtca tggcatgacg cagcgcgcgg ccctcctcga
     5701 acgcctgggg cagacgcggc tatccgccgc gcagcggcgt gtgatccagg cgcgaatcgc
     5761 gcgagacttt ccggacccgc ctgccgggcg tggcgcctga cgggagcgtt ctgctgcgga
     5821 ccgaagccgt gcagtgcgcg cgtcagatcg ccagcatccc gccgtccacg gcgacgatct
     5881 gcccggtgat gtagctcgac gcgtcggagg cgagcagcac ggccacgccc ttgagatcct
     5941 cgtcgccgcc caggcgcttc atgggcgtga gctgcatgat cgtgtccatg tggcgctcgg
     6001 ccatcacctt ggccatcttg gtggggaaca cgccgggcgc gatggcattc accgtgatgc
     6061 cgtattgcgc ccattcggcg gcgagcgagc gcgtgaagtt caccagtccg cccttgctgg
     6121 cgttgtaggc gatggtgcgc atgaagtccg gatgcgtgcc gttgaggccg gcgaccgagg
     6181 cgatgttgac gatgcggccg tagcgcgcgg ggatcatggc gcgcgtggcc gccgcctggg
     6241 tgagcgcgaa caccgccgtc aggttcaagt tgatcacctt gttccaggcg tccagcggat
     6301 gctcttccgc gggtgcgccc cacgtggcgc cggcgttgtt caccaggata tcgagccgcc
     6361 cgcgctcggc gaggaccttc tccaccagcg ccttggcggc gtgcgggtcc tgcaggtcgc
     6421 cggcgacggt gagcacctcg gccccctgcc cctgcagatg cgctgccgcc tcgtccagct
     6481 cgtgctgctt gcgggcggtg atcaccagcc tcgcccccat ctcccccaag gcctcggcga
     6541 tctgcagacc gaggccgcgc gaccctccgg tgatgagggc gacgc
//
LOCUS       DASPGE010000086         4505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_963720, whole genome shotgun sequence.
ACCESSION   DASPGE010000086 DASPGE010000000
VERSION     DASPGE010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963720"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..895
                     /gene="tpx"
                     /locus_tag="VD791_02730"
     CDS             281..895
                     /gene="tpx"
                     /locus_tag="VD791_02730"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HXV06918.1"
                     /translation="MKITASVGALALLAALAVPVLADDSMAVAKGSVAPGDGQSVTLK
                     GNALPLAGSAVKVGAPLPAATLVNGSMQPVNIAEAKGKVRIINVVPSLDTPVCDAQTH
                     ELVEKDPALAEAIELVTVSMDLPFAQQRWARAAKVKNVTFLSDHRDGNFGTNNGLLIQ
                     PLRLLARAVIVTDKDGVVRYLQVVPEITELPDMDAAMSAAKGLM"
     gene            977..1064
                     /locus_tag="VD791_02735"
     tRNA            977..1064
                     /locus_tag="VD791_02735"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1011..1013,aa:Ser,seq:tga)
     gene            complement(1052..2362)
                     /gene="rlmD"
                     /locus_tag="VD791_02740"
     CDS             complement(1052..2362)
                     /gene="rlmD"
                     /locus_tag="VD791_02740"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HXV06919.1"
                     /translation="MSVAVHIESLDQEGRGVARVDGKVTFVDGALPGEAVEVRAWRRK
                     RRFDLASATRILHASSQRVAPPCPHFERCGGCSLQHLDERAQVAIKQRTLEDSLARLG
                     GVRAEEILPPIHGPAWGYRLRGRLAVRHVAKKRGVLVGFHERRTHLVVEMDSCAVLPA
                     RISALILPLRELLGAMQLAARIPQVEFAAGEAADALVLRVLDPVGEHDLALLRGFAER
                     HALDIYLQPDGPDSVYPLLPERLRPLFYRLPDFDLTLHFRPGDFTQVNRAVNEVLVRR
                     AVGLLDPRPGQHLADLFCGLGNFTLALARRGAQVVGVEGCAELVARAEENARDNGLAQ
                     RARFRVADLFEAPQRVLAELGPLDGMLVDPPRQGAHRLVQALGEGAPRRVVYVSCNPA
                     TLARDAGVLVREKAYRLRAAGVVNMFPHTSHVESLALFERDGGG"
     gene            complement(2381..3205)
                     /locus_tag="VD791_02745"
     CDS             complement(2381..3205)
                     /locus_tag="VD791_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HXV06920.1"
                     /translation="MTPVLAFDVETIPDVAGLRLLHRIPTGISDRDVAEMAFHRRRQV
                     AGSEFLPLHLQRVVAVACALRERNAFTVWALGEPGDSEASLVQRFFDGIEKYTPQLVS
                     WNGGGFDLPVLHYRGLIGGVQARRYWDLGDDDREFRYNNYISRYHTRHLDLMDLLSMY
                     QPRATAPLDDIAQLLGFPGKLGMDGGQVWDAVQRGELARVRNYCETDAANTFLVFLRF
                     QLMRGALSPRQYQSELELVHSTLARSSAAHWQEFLAAWDIAHGGDVAHGASSAASG"
     gene            3345..>4505
                     /locus_tag="VD791_02750"
     CDS             3345..>4505
                     /locus_tag="VD791_02750"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HXV06921.1"
                     /translation="MGNPARLEPASSEKPEIPDHVRHHRLREWVMQAARLTRPERIVW
                     CDGSDAEYATLCVELTAAGTFVALDRAKRPGSYLARSDPSDVARVEDRTFVCSIHRAD
                     AGPTNNWMEPGQMKARLARLFDGCMRGRTMYVLPFSMGPIGSPISHIGVEITDSAYVA
                     ASMRIMTRMGRAVVEALGEDGEFVACLHSVGMPLSPGQSDVPWPCNREHKYIVHFPEE
                     NAIWSFGSGYGGNALLGKKCFALRIASAMAREQGWLAEHMLILGVESPAGEKTYVAAA
                     FPSACGKTNPAMMVSALQDQGYRVWTVGDDIAWMTIGKDGYLHAINPEAGFFGVAPGT
                     NSQTNPNVIDALHTNSMFTNTAMTSSREPWWEGMDSAPPAGLVDWQGRTADAA"
BASE COUNT          736 a         1576 c         1539 g          654 t
ORIGIN      
        1 tccaggtgat cgcctacctg aagacgcgcg agccgcagct ctacaagcgg ctgcggcagc
       61 aggcggtgag cggcggcaac tcggtacaca agcgcgcccg gcaccgctga ggtgcaggtg
      121 ccggggctgc ccggctcgtc ccgccccggg actgcgcggg tgtggcagcg tatactcccc
      181 ggtccggggc gtgtacgcac ccggcgccgc ggaggcgcct tgcccgtgtc atcggcgggc
      241 ggcacgagtt cgttgcgtcc ataccaacaa cggaggaacc atgaagatca ccgcatccgt
      301 cggcgcgctg gcgctgctcg ccgcgctcgc cgtccccgtc ctagccgacg acagcatggc
      361 cgtggccaag ggcagcgttg cccccggcga tggccagtcg gtcaccctga agggcaatgc
      421 cttgccgttg gccggaagcg cggtcaaggt cggcgcgccc ctgcccgccg ccaccctggt
      481 gaacggcagc atgcagccgg tcaacatcgc cgaagccaag ggcaaggtgc gcatcatcaa
      541 cgtcgtgccg tcgctggaca ccccggtgtg cgacgcgcag acccacgaac tggtggaaaa
      601 agacccggcg ctagccgagg ccatcgagct ggtgacggtg agcatggacc tgccgttcgc
      661 gcagcagcgc tgggcccgtg cggccaaggt caagaacgtc accttcctgt ccgatcaccg
      721 cgacggcaac ttcggcacga acaacggcct gctgatccag ccgctgcgcc tgctcgcccg
      781 tgccgtcatc gtcaccgaca aggacggcgt ggtgcgctac ctgcaggtcg tcccggaaat
      841 caccgagctg cccgacatgg acgccgcgat gagcgcggcc aaagggttga tgtaacagac
      901 ccccgacctc acgaaaggcc ctgcctaagg cagggccttt tcgtttccgg cattccgatc
      961 gcgtatcatt ggttccggag ggttggcaga gcggtcgaat gcggcggtct tgaaaaccgt
     1021 tgtggggcaa cccaccgggg gttcgaatcc ctcaccctcc gccatctctc tcgaacaacg
     1081 ccagcgactc gacgtgcgag gtgtgcggga acatgttcac cacgcccgcg gcgcgcagcc
     1141 gataggcttt ctcgcgcacc agcacgccgg catcgcgcgc gagcgtcgca ggattgcacg
     1201 acacgtacac caccctgcgc ggcgcccctt cgcccagcgc ctgcaccagg cggtgggcgc
     1261 cctgtcgcgg cggatcgacg agcatgccgt cgagcgggcc gagctccgcg aggacgcgct
     1321 gcggggcttc gaacaggtcg gcgacgcgga accgcgcgcg ctgcgccaat ccgttgtccc
     1381 gcgcgttctc ctccgcgcgc gccaccagct ccgcgcagcc ctccactccc accacttgcg
     1441 ccccgcggcg cgccagcgcg agggtgaaat tgcccaggcc gcagaacagg tcggcgaggt
     1501 gctggccggg acgcgggtcg agcagaccga cggcgcgccg caccagcacc tcgttcactg
     1561 cgcggttgac ctgggtgaag tcgcccggtc ggaaatgcag cgtgaggtcg aagtcgggca
     1621 gccggtagaa cagggggcgc agccgttccg gtagcagggg gtacaccgag tccggtccat
     1681 ccggctgcag gtagatgtcc agcgcgtggc gctcggcgaa cccgcgcagc agcgcgagat
     1741 cgtgctcgcc caccggatcg agcacgcgca gcaccagcgc atcggccgcc tcgcccgcgg
     1801 cgaactcgac ctggggaatg cgcgccgcga gctgcatggc tccgagcaac tcgcgcagcg
     1861 gcaggatcag agccgagatg cgcgcgggca gcaccgcgca cgagtccatc tccaccacca
     1921 gatgcgtgcg gcgctcgtgg aagccgacca gcacgccgcg cttcttcgcg acgtggcgca
     1981 ccgcgaggcg cccccgcagg cgatagcccc acgccggtcc gtggatcggc ggcaggatct
     2041 cctcggcgcg cacgccaccg aggcgcgcca ggctgtcctc cagcgtgcgc tgcttgatcg
     2101 ccacctgcgc gcgctcgtcc aagtgctgca ggctgcagcc gccgcagcgt tcgaaatgcg
     2161 ggcagggcgg ggcgacgcgc tggctgctcg cgtgcaggat gcgggtggcg ctcgccagat
     2221 cgaagcggcg cttgcgccgc caggcccgga cctcgaccgc ctcgcccggt agcgcgccgt
     2281 cgacgaaggt caccttgccg tccacgcggg cgacgccgcg cccttcctga tccagggact
     2341 cgatgtggac ggcgacgctc aacgtgctag cccggctatt tcatccggac gcggcgctgg
     2401 acgcgccgtg cgcgacgtcc ccgccgtgcg cgatatccca ggcggcgagg aattcctgcc
     2461 aatgcgcggc gctcgaccgc gccagcgtgc tgtgcaccag ctccagttcg ctctggtact
     2521 gccgcgggct gagcgctccg cgcatgagct ggaagcgcag gaacaccagg aaggtgttgg
     2581 cggcgtccgt ctcgcagtag ttgcgcaccc gcgcgagctc gccgcgttgc acggcatccc
     2641 acacctgccc gccgtccatg ccgagcttgc ccgggaagcc gagcagttgt gcgatgtcgt
     2701 cgagcggtgc ggtggcgcgc ggctgataca tggacaacag gtccatcaga tccaggtggc
     2761 gcgtgtggta gcggctgatg tagttgttgt agcggaactc gcggtcgtcg tcacccaggt
     2821 cccagtagcg ccgcgcctgc acgccgccga tcaacccccg gtaatgcagc accggcaggt
     2881 cgaagccgcc gccgttccag gaaaccagct gcggcgtgta cttctcgatg ccatcgaaga
     2941 agcgctgcac caggctggcc tccgaatcgc ccggttcgcc cagcgcccac acggtgaagg
     3001 cgttgcgctc gcgcaacgcg caggcgaccg ccaccacgcg ctgcagatgc aacggcagga
     3061 actcgctgcc ggcgacctgg cggcggcgat ggaacgccat ttccgccacg tcgcggtcgc
     3121 tgatgccggt cgggatacga tgcagcaggc gcagcccggc gacatccgga atggtctcga
     3181 catcgaaggc gaggacgggt gtcatggtat gcagcgaatc gtgtaaggat tgtatatcgg
     3241 gaggtggacg gcgcgccgga cgtgctacca tgcattgcgc acgacgcccc gggtatctac
     3301 aggaatcggg ggcatcgaca gaaatcggga gggagacacc aagcgtgggc aacccagcgc
     3361 gcctcgagcc ggcctcctcc gaaaagccgg agattcccga ccacgtccgc catcaccggc
     3421 tgcgcgagtg ggtgatgcag gccgcgcgcc tgacccggcc ggagcgcatc gtctggtgcg
     3481 acggctcgga tgccgagtac gccacgttgt gcgtggagct caccgccgcc ggcacgttcg
     3541 tggcgctcga ccgcgcgaag cgccctggca gctacctcgc tcgctccgac ccttccgacg
     3601 tcgcccgcgt cgaggaccgc accttcgtgt gcagcatcca ccgcgcggac gccggcccca
     3661 ccaacaactg gatggagccc gggcagatga aagcccggct ggcgcggctg ttcgacggct
     3721 gcatgcgcgg gcgcacgatg tacgtgctgc cgttctcgat ggggccgatc ggctcgccca
     3781 tctcgcacat cggcgtggag atcaccgact cggcttacgt cgccgccagc atgcgcatca
     3841 tgacgcgcat gggccgcgcc gtggtggagg cgctgggcga ggacggcgag ttcgtcgcct
     3901 gcctgcactc ggtgggcatg ccgctctctc ccggtcagtc cgacgtgccg tggccgtgca
     3961 atcgcgagca caagtacatc gtgcactttc cggaggagaa cgcgatctgg tcgttcggca
     4021 gcggctacgg cggcaacgcg ctgctgggca agaagtgctt cgcgctgcgc atcgcctcgg
     4081 ccatggcacg cgagcagggc tggctggccg agcacatgct gatcctgggc gtggagtcgc
     4141 cggccggcga gaagacctat gtggcggccg cgttccccag cgcctgcggc aagacgaacc
     4201 cggccatgat ggtttccgcc ctgcaggatc agggctatcg cgtctggacg gtcggcgacg
     4261 acatcgcctg gatgaccatc ggcaaggacg gctacctgca cgcgatcaat cctgaggcag
     4321 gtttcttcgg cgtcgcgccg ggcaccaact cacagaccaa tcccaacgtg attgacgcct
     4381 tgcatacgaa ctcgatgttc accaacacgg ccatgacgtc ctcgcgcgag ccgtggtggg
     4441 aaggcatgga cagcgcgcca ccggcgggct tggtggactg gcagggacgc acagcggatg
     4501 ctgcc
//
LOCUS       DASPGE010000087         8664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_964433, whole genome shotgun sequence.
ACCESSION   DASPGE010000087 DASPGE010000000
VERSION     DASPGE010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8664
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964433"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VD791_02755"
     CDS             <1..321
                     /locus_tag="VD791_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06922.1"
                     /translation="GNVAIMGATMYVGGAELREALQAAAEGAPERISAEAANKAATAL
                     LLSLRACLANMLPFLLYGALMSALFFVALLPLFVGLAVWVPIAVISAYTSYRDVFGSA
                     PTPA"
     gene            complement(308..2512)
                     /locus_tag="VD791_02760"
     CDS             complement(308..2512)
                     /locus_tag="VD791_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXV06923.1"
                     /translation="MSSSLLEGLNERQRAAVTLPHESALILAGAGSGKTRVLTTRIAW
                     LIQTGQIGPQSLLAVTFTNKAAREMLTRISAMLPINTRGMWVGTFHGLCNRMLRAHWR
                     DAGLPQTFQILDTQDQASLIKRMLKSLGVDEEKVPPRDLQYFINGNKEQGLRAGQVEV
                     FDDHDRKRNELYAEYDRQCNREGVVDFAELLLRCYELLGRNELLRAHYQQRFVHILVD
                     EFQDTNRLQYLWLKLLAGGGSAVFAVGDDDQSIYAFRGASAQNMADFEHDFARGRVIK
                     LEQNYRSHGNILDAANALIRHNQRRLGKELWTAEGKGEPLRLYEAATDIEEASFILEE
                     VKGLRGDSVPLSDIALLYRSNAQSRVLEHALFNAGLPYRVYGGLRFFERQEIKHALAY
                     LRLVSNPEDDGALLRVINFPARGIGARTLEQMQESAHMRRVSLWQAACTTPLGGKAGA
                     GVGAFVQRIEELRRACLGLSLPEMVELTIERSGLKTYYETERDGADRIENLNELVTAA
                     ASFHAEGGFAAPAEPAAGAETEADPRLRALDDFLAHAALEAGEHQAGAGADALQLMTV
                     HAAKGLEFHAVFISGLEEGLFPHDNSRNEPESLEEERRLMYVALTRARRRLYLSFAQS
                     RMLHGQTRYNIASRFLDEVPAELVRRLNRGRAAGPFAPGATRSMGVASTGPYAAASDS
                     PWRIGQSVIHPKFGPGVIVSAEGRGADARVQVNFRSEGLKWLALEYAKLTPA"
     gene            complement(2575..3468)
                     /locus_tag="VD791_02765"
     CDS             complement(2575..3468)
                     /locus_tag="VD791_02765"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HXV06924.1"
                     /translation="MTMLLRSAARLTLLLATAALPALAAPPDAEVEALQMPAWLTRDG
                     QRTPLAPGARLRNGDRIDTGPGSRALLRLPDGSLVKLGEKARFSLDGVSGARRADGLF
                     KATLNVLQGAFRFTTTALYKFRGRREVEVRFPTVTAGIRGTDLWGKATAEREIVALIE
                     GRITVTRRGTPPLDLDQPRSLYQALRNAGVLPVEQISDEALAVYAAETEIAPGQGAAG
                     KGGKWRVYAARVRTQDEALAVYDRLREAGYPATIHPTQKEGTTVYQVRIGGLLSEADG
                     VAVAIRLKSELGLTEVSVSLI"
     gene            3625..6240
                     /gene="leuS"
                     /locus_tag="VD791_02770"
     CDS             3625..6240
                     /gene="leuS"
                     /locus_tag="VD791_02770"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HXV06925.1"
                     /translation="MQPQYRPADIEKQAQAEWDEKRAARAVEDPARPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLARYYRMRGHNVLQPMGWDAFGLPAENAAMANGVPPAKWTY
                     DNIAYMKQQLKSLGFAIDWERELATCRPEYYRWNQWLFVRMLEKGLAYKTTGAVNWDP
                     VDQTVLANEQVIDGRGWRTGAQVEKREIPMYYLRITAYADELLQGLETLPEWPERVRL
                     MQSNWIGRSEGVEVAFPYAHDPAQALKVFTTRADTLLGATYCAVAAEHPLAAQAARGK
                     PELAAFVEVCKRGAVMEAELATQEKKGMPTGLSVLHPLTGEKLPLWVANYVLMGYGEG
                     AVMAVPAHDERDFEFATRYGLPIKPVIRTSAGETTPAPWRDAYAEHGTLINSGKYDGL
                     AFQPAVDAIAADLAAKGLGRKRIQYRLRDWGISRQRYWGCPIPIVHCPQCGDVPVPDA
                     QLPVVLPEDCVPDGAGNPLNRRADFVNIRCPKCDQPARRETDTMDTFVDSSWYYIRYA
                     CPDNDRAMVDERVRYWLPVDQYIGGIEHAILHLLYSRFWTRVMRDLGLVRFDEPFSRL
                     LTQGMVLNEIFFRKQENGRIAYYNPADVEIRTDDRGNRIGVALKVDGLPLESGGVGTM
                     SKSKNNGVDPQALVDQYGADTARFFMMFTSPPEQTLAWSDSGVEGSYRFLRRLWAFAA
                     DYQGSVRPQVRPGALAQSALDAPLAAVRREVHLNVKQANYDMQRHQFNTVASACMKIL
                     NALEGVPRNPPPIGPGAAAVVEEGLSILLRLQAPITPHLSHHLWRELGFGEDVFAAPW
                     PQAEESALEQKEIELVLQINGKTRGSIRVPSDATREAIEQAALASPVTQKYLAGAKVK
                     KIVVVPGRLVNVVTQ"
     gene            6255..6740
                     /gene="lptE"
                     /locus_tag="VD791_02775"
     CDS             6255..6740
                     /gene="lptE"
                     /locus_tag="VD791_02775"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HXV06926.1"
                     /translation="MRLLALVALAALAGGCGFKPVAPLNVPFHNLYVVAPDYSSFGAQ
                     FKRYVESYSKTRLASSPETAAAVLEILSETREKQILSLSTAGRVSEFLLRYQVTFRVR
                     DKSSRDLVPASTIALERDLTFSDEATLGKENEEVFLYDDMRNEAIQQVLGRLSAAQIP
                     A"
     gene            6748..7917
                     /locus_tag="VD791_02780"
     CDS             6748..7917
                     /locus_tag="VD791_02780"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HXV06927.1"
                     /translation="MPDVLAAVMPAPAGAEPGSVGVVRAQRAAFDAPLQLRSGASIAA
                     YELAYETYGTLNASRSNAVLVCHALNAAHHVAGVYADDPKNVGWWDNLVGPGKPLDTD
                     RFFVVGVNNLGGCHGSTGPASVNPASGRRWGADFPLVTVEDWVEVQARLADRLGIERF
                     AAAIGGSLGAMQALQWTLSYPDRIRHALVIAAAPKLSAQNIAFNEVARQAITTDPDFH
                     GGHYYEHGVVPRRGLRLARMLGHITYLSDDAMAEKFGRALRTGAIKFNFDVEFEIESY
                     LRHQGDKFSEYFDANTYLRITKALDYFDPAADFGGDLTRALARAKAAFLVVAFTSDWR
                     FSPARSREIVKALIDNRRDVTYAEIEAVHGHDAFLMDDPNYHGVLRAYFDNIDIR"
     gene            7923..8549
                     /gene="metW"
                     /locus_tag="VD791_02785"
     CDS             7923..8549
                     /gene="metW"
                     /locus_tag="VD791_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HXV06928.1"
                     /translation="MPAAALHSHPRLSGRHDFDAIAQWIPEGARVLDLGCGDGSLLRF
                     LRQTRRASGYGVEIDEAKLIAGVRNGVNVIHSDLESGLSGFESRSFDYVILSLTLQAV
                     RHTERIVKEMLRVGRESIVSFPNFGFWRHRAQVLGGRMPVSRHLPYHWYDTPNVHLFT
                     IRDFEAFCADHGIRILERVVMDEAGHDIRRLPNLLGALAVYRLDAGSR"
BASE COUNT         1321 a         2972 c         2988 g         1383 t
ORIGIN      
        1 ggcaacgtgg cgatcatggg cgcgaccatg tacgtcggcg gggcggagct gcgcgaggcg
       61 ctgcaggccg ccgccgaggg cgcaccggag cgcatctctg ccgaagcggc caacaaggcg
      121 gccacggcgc tgctgctcag cctgcgggcc tgcctcgcca atatgctgcc gttcctgctc
      181 tacggcgcgc tgatgtcggc gctgttcttc gtggcgctgc ttccgctgtt cgtcgggttg
      241 gcggtatggg tgccgatcgc ggtgatcagc gcctacacca gctatcggga cgtgttcggc
      301 tcggccccta cgccggcgtg agcttggcgt attccagcgc cagccacttc agcccctcgc
      361 tgcggaagtt cacctgcacg cgggcatcgg cgccgcgccc ctcggcgctg acgatcacgc
      421 cggggccgaa cttggggtgg attacgctct ggccgatgcg ccagggcgaa tcggacgccg
      481 ccgcgtacgg tccggtgctc gcgaccccca tggagcgcgt cgcaccgggc gcaaacgggc
      541 ccgcggcgcg gccccggttc agccgcctca ccagctccgc gggcacttcg tccaggaaac
      601 gcgaggcgat gttgtagcgc gtctggccgt gcagcatgcg gctctgggcg aagctcaggt
      661 acagccggcg gcgcgcgcgc gtgagcgcca cgtacatcag gcgccgctcc tcctccaggc
      721 tttccggctc gttgcggctg ttgtcgtggg ggaacagccc ttcctccagg ccgctgatga
      781 acaccgcgtg gaactccagg cccttggccg cgtgcaccgt catcagctgc agcgcatcgg
      841 cgccggcacc ggcctggtgc tccccggcct cgagcgccgc atgggccaga aagtcgtcga
      901 gcgcacgcag gcgcggatcg gcctcggttt cggcgcctgc cgccggttcg gcgggcgcgg
      961 cgaaaccgcc ctcggcgtgg aagctggcgg cggcggtgac cagctcgttc aggttttcga
     1021 tgcgatcggc gccgtcgcgc tcggtctcgt agtacgtctt gaggccgctg cgttcgatgg
     1081 tgagctcaac catctcgggc agcgacaagc ccaggcaggc gcgccgcagc tcctcgatgc
     1141 gctgcacgaa ggcgccgacg ccggctcccg ctttgccgcc gagcggcgtg gtgcaggccg
     1201 cctgccacag gctgacccgg cgcatgtgcg ccgactcctg catctgttcg agggtgcgcg
     1261 cgccgatacc gcgcgcggga aagttgatca cgcgcagcaa ggcgccgtcg tcctccggat
     1321 tggacaccag ccgcaggtag gcgagcgcgt gcttgatctc ctggcgctcg aagaagcgca
     1381 acccgccgta cacgcggtac ggcagtccgg cgttgaacag cgcgtgctcc agcacccgcg
     1441 actgcgcgtt ggagcggtac agcagcgcga tgtcggaaag cggcacgctg tcgccgcgca
     1501 gccccttcac ctcctccagg atgaaggacg cctcctcgat gtcggtggcg gcctcgtaca
     1561 ggcgcagcgg ctcgcccttg ccctcggcgg tccacagctc cttgcccagc cggcgctggt
     1621 tgtggcggat cagggcgttg gccgcatcca ggatgttgcc gtggctgcgg tagttctgct
     1681 cgagcttgat cacgcgccct ctggcgaagt catgctcgaa gtccgccatg ttctgcgccg
     1741 aggcgccgcg gaaggcgtag atcgactggt cgtcgtcgcc cacggcgaag acggcgctgc
     1801 cgccgccggc cagcagcttg agccagaggt attgcaggcg gttggtgtcc tggaactcgt
     1861 ccaccaggat gtgcacgaag cgctgctggt aatgggcgcg cagcagctcg ttgcggccga
     1921 gcagctcgta acagcgcagc agcagctcgg cgaaatccac cacgccctcg cggttgcatt
     1981 gccggtcgta ctcggcgtac agctcgttgc gcttgcggtc gtgatcgtcg aacacctcca
     2041 cctggccggc gcgcaggccc tgctccttgt tgccgttgat aaagtactgc aggtcccggg
     2101 gcgggacctt ctcctcgtcc acgcccaggg acttgagcat gcgcttgatc agggaggcct
     2161 ggtcctgcgt gtccaggatc tggaacgtct gcggcaggcc ggcgtcgcgc caatgcgcgc
     2221 gcagcatgcg attgcacagc ccgtggaagg tgcccaccca catgccgcgg gtgttgatcg
     2281 gcagcatggc cgagatgcgg gtcagcatct cgcgcgcggc cttgttggtg aacgtcaccg
     2341 ccagcagcga ctgcggtccg atctgaccgg tctggatcag ccaggcgata cgcgtggtga
     2401 gcacgcgcgt cttgccgctg ccggcgccgg cgaggatgag cgcggattcg tgcggcaggg
     2461 tgacggcagc gcgctgccgt tcgttgagac cttcgaggag gctgctcgac acgaaacctg
     2521 gaggcgtgag tgtgtgccgc aattatacgg cagcgctcgc gcgttacgat tgcgctagat
     2581 cagcgacacg ctgacttcgg tgaggccgag ctcggacttg agcctgatcg ccaccgccac
     2641 cccgtcggcc tcgctcagca gcccgccgat gcgcacttgg tagacggtcg tcccttcctt
     2701 ctgggtcgga tggatcgtcg ccggatagcc ggcttcgcgc agccggtcgt acacggcaag
     2761 cgcctcgtcc tgggtgcgca cgcgcgctgc gtacacgcgc cacttgccgc ctttgccggc
     2821 ggcgccctgg cccggcgcga tctcggtctc ggcagcgtac acggcgagcg cctcgtccga
     2881 gatctgttcc accggcagca cgccggcgtt acgcagcgcc tggtacaggc tcctgggctg
     2941 atccaggtcg agcggcggcg tcccgcgccg cgtgaccgtg atccggcctt cgatcaatgc
     3001 gacgatctcg cgctccgcgg tcgccttgcc ccataggtcg gtgccgcgga tgccggcggt
     3061 gacggtggga aagcgcactt cgacctcgcg cctgccgcgg aacttgtaca gcgccgtggt
     3121 ggtgaagcgg aatgcgccct gcagcacgtt gagcgtggct ttgaacagcc cgtcggcacg
     3181 gcgcgcgccg ctgacgccgt ccaggctgaa gcgcgccttc tcgcccagct tcaccaggct
     3241 gccgtcgggc aggcgcagca gcgcccgcga gccggggccg gtgtcgatgc gatcgccgtt
     3301 gcgcagtcgg gcgcccggcg cgagcggcgt gcgctggccg tcgcgcgtga gccaggcggg
     3361 catctgcagc gcctcgacct cggcatccgg cggcgcggcg agggccggca gggcggcggt
     3421 cgccagaagc agcgtcaggc gcgcggccga tcttagcagc atggtcattg catctctcca
     3481 tgacgttgta gccgatgcgt gcccgagata cggggcgcgc ggtggaccgg atgcgctgcg
     3541 cacagcgctt tcatagcggt ttcggcagcg cgagccccct gggtataatt ccgcccttgc
     3601 cctcacttcg cgcagtgtag acgaatgcaa ccgcagtacc gcccggcaga catcgagaag
     3661 caggcgcagg ccgaatggga cgagaagcgc gccgcgcggg cggtcgagga cccggcgcgg
     3721 cccaagtact actgcctgtc catgttcccc tacccctctg gcaagctgca catggggcac
     3781 gtgcgcaact acaccatcgg cgacgtgctg gcgcgctact accgcatgcg cggccacaac
     3841 gtgctgcagc cgatgggctg ggacgccttt ggcctgcccg cggagaacgc cgccatggcc
     3901 aacggcgtgc cgccggcgaa gtggacctac gacaacatcg cctacatgaa gcagcagctc
     3961 aagtcgctgg gcttcgccat cgactgggaa cgggaactcg ccacctgccg gcccgagtac
     4021 taccgctgga accagtggct gttcgtgcgc atgctggaga aggggctcgc ctacaagacc
     4081 accggcgcgg tgaactggga tccggtcgac cagaccgtgc tcgccaacga gcaggtgatc
     4141 gacgggcgcg gctggcgcac cggcgcgcag gtggagaagc gcgagatccc aatgtactac
     4201 ctcaggatca ccgcctatgc ggacgagctg ctccagggcc tggagacgct tccggagtgg
     4261 ccggagcgcg tgcgcctgat gcagtcgaac tggatcgggc gcagcgaagg cgtcgaggtc
     4321 gcgtttccct acgcccacga tccggcgcag gccctcaagg tgttcaccac ccgcgccgac
     4381 acgctgctcg gcgccaccta ttgcgcggtg gcggcggagc atccgctcgc ggcgcaggcc
     4441 gcgcgcggca agcccgagct ggccgcgttc gtcgaggtgt gcaagcgcgg ggcagtgatg
     4501 gaagccgaac tcgccaccca ggagaagaag ggcatgccca ccgggctctc cgtgctgcat
     4561 ccgctcaccg gggagaagtt gccgctatgg gtggcgaact acgtgctgat gggctacggc
     4621 gagggtgcgg tgatggcggt gccggcgcac gacgagcgcg acttcgagtt cgccacccgc
     4681 tacggcctgc cgatcaaacc ggtcatccgc accagcgccg gcgagaccac gcccgcgccc
     4741 tggcgcgacg cctatgccga gcacggcacg ctgatcaact cgggcaagta cgacgggctg
     4801 gcgttccagc ccgcggtgga cgcgatcgcc gccgatctgg cggcaaaggg cctggggcgc
     4861 aagcgcatcc agtaccgcct gcgcgattgg ggcatctcgc gccagcgcta ctggggctgc
     4921 cccatcccca ttgtgcactg cccgcaatgc ggcgacgtgc cggtgcccga cgcgcagctg
     4981 ccggtggtgc tgcccgagga ctgcgttccc gacggcgccg gcaatcccct caaccggcgc
     5041 gccgacttcg tcaacatccg ctgccccaag tgcgaccagc cggcgcggcg cgagaccgac
     5101 accatggaca ccttcgtgga ctcgtcctgg tattacattc gctacgcctg tccggacaac
     5161 gaccgcgcca tggtggacga gcgcgtgcgc tactggctgc cggtggacca gtacatcggc
     5221 ggcatcgagc acgccatcct gcatctgctg tattcgcgct tctggacgcg cgtgatgcgc
     5281 gatctcggcc tggtgcgctt cgacgagccg ttctcgcgcc tgctcaccca gggcatggtg
     5341 ctcaacgaga tcttcttccg caagcaggag aacgggcgca tcgcctacta caacccggcg
     5401 gacgtggaga tacgcaccga cgaccgcggc aaccggatcg gcgtcgcgct gaaggtcgac
     5461 ggcttgccgc tggaatcggg cggcgtcggc accatgtcca agtccaagaa caacggcgtc
     5521 gacccgcagg cgctggtgga tcagtacggc gccgacaccg cgcgcttctt catgatgttc
     5581 accagcccgc cggagcagac gctggcgtgg tccgactccg gcgtggaagg ctcctaccgc
     5641 ttcctgcgcc ggctatgggc gttcgccgct gactaccagg gcagtgtgcg gccgcaggtc
     5701 cggcccggcg cgctggcgca gagcgcgctc gacgcgccgc tggccgccgt gcgccgggag
     5761 gtgcatttga atgtcaagca ggccaactac gacatgcagc gccaccagtt caacacggtg
     5821 gcgtcggcct gcatgaagat cctgaatgcg ctggagggcg tgccgcggaa cccgcctcca
     5881 atcggacccg gcgccgccgc ggtcgtcgag gaagggctgt cgatcctgct gcgcctgcag
     5941 gcgccgatca cgccgcatct cagccaccat ctgtggcgcg agctggggtt cggcgaggac
     6001 gtgttcgccg cgccctggcc gcaggccgag gagagtgcgc tggagcagaa ggagatcgag
     6061 ctggtgttgc agatcaacgg caagacgcgc ggcagtatac gcgtgccgag cgacgctacc
     6121 cgggaagcga tcgagcaggc cgcgttggcc agcccggtga cgcaaaaata cctggccggt
     6181 gcgaaagtga agaagattgt ggtggttccc ggccgcctgg tgaacgtggt cacgcaatga
     6241 gaccggcgtt gcgcatgcga ctgttggctc tcgtcgcgct ggctgcgctc gcgggtgggt
     6301 gcggcttcaa gccggtggcg ccgctgaacg tgccgttcca caatctctac gtggtggccc
     6361 cggactactc ctcgttcggc gcccagttca agcgctacgt ggagagctac agcaagacgc
     6421 gcctggcttc cagcccggag acggccgcgg cggtgctgga gatcctgagc gagacgcggg
     6481 agaaacagat cctgtccctg tccaccgccg ggcgcgtgag cgagttcctg ctgcgctacc
     6541 aggtgacctt ccgggtgcgc gacaagagca gccgcgacct ggttccggcc agcaccatcg
     6601 cgctggagcg cgatctgacc ttcagcgacg aggccacgct gggcaaggag aacgaggaag
     6661 tcttcctcta cgacgacatg cgcaacgagg cgatccagca ggtgctgggg cggctgtcgg
     6721 cggcgcagat tcccgcgtga gcgcgcgatg ccggacgtcc tcgccgccgt catgccagcg
     6781 cccgccgggg ccgagcccgg gtcggtgggg gtggtccgcg cacagcgcgc cgcgttcgat
     6841 gcgccgctgc agctgcgcag cggcgcctcc atcgcggcgt acgaacttgc ctacgaaacc
     6901 tacggcacgc tgaacgcgag ccgcagcaat gcggtgctgg tgtgccacgc gctgaacgcc
     6961 gcgcaccacg tcgccggcgt ctacgccgac gatcccaaga acgtcggctg gtgggacaac
     7021 ctggtgggcc cgggcaagcc gctcgacacc gaccgcttct tcgtggtcgg ggtgaacaac
     7081 ctgggcggct gccacggttc cacgggcccg gcctcggtca acccggccag tggccggcgc
     7141 tggggcgccg atttcccgct ggtgacggtg gaagactggg tcgaggtgca agcgcgcctg
     7201 gccgaccggc tcgggatcga gcgcttcgcc gcggccatcg gcggcagcct gggcgcgatg
     7261 caggcgctgc agtggacgct gagctacccg gatcgcatcc gccacgcgct ggtgatcgcg
     7321 gcggcgccca agctgtcggc gcagaacatc gccttcaacg aggtcgcccg ccaggccatc
     7381 accaccgacc ccgacttcca tggcggccac tactacgagc acggcgtagt gccgcggcgc
     7441 gggctgcgcc tggcgcggat gctgggccac atcacctacc tgtccgacga cgcgatggcc
     7501 gagaaattcg gccgcgcgct gcgcaccggt gcgatcaagt tcaacttcga cgtcgagttc
     7561 gagatcgagt cctacctgcg ccaccagggc gacaagttct ccgagtattt cgacgccaac
     7621 acctacttgc gcatcaccaa ggcgctggat tacttcgatc ccgcggccga cttcggcggc
     7681 gacctgaccc gggcgctggc gcgggccaag gccgcgttcc tggtggtggc ctttacttcc
     7741 gactggcgct tctcgccggc gcgctcgcgc gagatcgtca aggcgctgat cgacaaccgg
     7801 cgcgacgtga cttacgccga gatcgaggcg gtgcacgggc acgacgcgtt cctgatggac
     7861 gacccgaact accacggcgt gctgcgcgcc tatttcgaca acatcgacat ccggtgacgg
     7921 gtatgcccgc ggccgccctg cattcgcatc cgcgcctgtc cggccggcac gacttcgacg
     7981 ccattgcgca gtggatcccg gagggcgcgc gcgtgctcga cctcggctgc ggcgacggct
     8041 ccctgctgcg tttcctgagg cagacccggc gcgcgtccgg ctacggcgtc gagatcgacg
     8101 aggccaagct gatcgccggg gtgcgcaacg gggtcaacgt gatccacagc gacctcgagt
     8161 ccggcttgtc cggcttcgag tcccgctcct tcgactacgt gatcctgtcg ctcaccctgc
     8221 aggcggtgcg ccataccgag cgcatcgtga aggagatgct gcgcgtgggc cgggagagca
     8281 tcgtcagctt tcccaacttt ggcttctggc gccaccgcgc gcaggtgctg ggcgggcgca
     8341 tgccggtttc gcggcacctg ccgtaccact ggtacgacac gcccaacgtg cacctgttca
     8401 ccatccgcga cttcgaggcg ttttgcgcgg accacggcat ccgcatcctg gagcgcgtgg
     8461 tgatggacga ggccggccac gacatccgcc ggttgcccaa tctgctcggc gcgctcgccg
     8521 tgtatcgcct cgacgccggg tcccgctagc ccggatattt catcaggacg cggaattatg
     8581 gcctgttctg gacgagtcag atcgggctcg accgccgcag ccaatagatc attctattgg
     8641 caaggcgggc atgcccgaga tggc
//
LOCUS       DASPGE010000088         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1432956, whole genome shotgun sequence.
ACCESSION   DASPGE010000088 DASPGE010000000
VERSION     DASPGE010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432956"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1219
                     /gene="glnL"
                     /locus_tag="VD791_02790"
     CDS             170..1219
                     /gene="glnL"
                     /locus_tag="VD791_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HXV06929.1"
                     /translation="MAAAFSGLELLATAVLVLDAQQLVRYANPAAENLLGAGAKSLIG
                     QPFLQLFAPDSALEKALSDALVTHWDYSAQTVSYPRPGREPLPLSCTLTRAEAPGVSL
                     LAELRPIEQQLRQAREERLASEQQSTRELIRNLAHEIKNPLGGLRGSAQLLEHELDRA
                     ELREYTQVIIKEADRLQTLMDRLLTPHRQPRVERLGIHEVLERVRSLVLAEFARAAVE
                     IECDYDPSLPDLEGDKEQLIQAVLNVVRNAAQAVAGSGRPGRITLRTRAIRQVTLLRQ
                     RHRLALELQVIDNGPGVPEDIREKIFNPLVSGRDGGSGLGLSLAQTFVHYHRGVIECD
                     SRPGRTVFRIVLPLA"
     gene            1216..>2077
                     /locus_tag="VD791_02795"
     CDS             1216..>2077
                     /locus_tag="VD791_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXV06930.1"
                     /translation="MKPVWIVDDDRSIRWVFEKALGREGIAFNSFSSADEALHALRSG
                     PPQVLVSDIRMPGPSGLELLQTVKQQHPAVPVIVMTAYSDLESAVAAFQGGAFEYLPK
                     PFDVDQAVELIRRALDESQRDERAAEPPAEAPEILGQAPAMQEVFRAIGRLSQSSATV
                     LITGESGSGKELVARALHRHSARAARPFVAINTAAMPKDLLESELFGHERGAFTGAQQ
                     LRRGRFEQAEGGTLFLDEIGDMPADLQTRLLRVLSEGSFYRVGGHQPIKTNVRVIAAT
                     HQNLEARVREG"
BASE COUNT          332 a          723 c          736 g          286 t
ORIGIN      
        1 aggagctgga ggagcaggag tccgtgcgct acggcgacga acgcaactac gcgcgggtcc
       61 tcgagcgcct gcagaagtac aaggacgccg tggaactgca cgagaagaac atcgaatcgc
      121 tcaggcgcga gctgaccatc ctgtaccggt gaaccggccg gcctgaccga tggccgcggc
      181 cttttccgga ctcgagctgc tggccaccgc ggtgctcgtg ctcgacgcgc agcagctcgt
      241 gcgctacgcc aatcccgcgg ccgagaacct gctcggcgcg ggcgcgaaga gcctcatcgg
      301 ccagccgttc ctgcagctgt ttgcgcccga cagcgccctc gagaaggcgc tgtccgatgc
      361 gctggtgacg cactgggact actccgcgca gacggtgagc tacccgcgcc cgggccgcga
      421 gccgctgccg ctttcctgca cgctgacgcg cgccgaggcg cccggcgtgt cgctgctggc
      481 cgagctgcgc ccgatcgagc agcagctgcg ccaggcgcgc gaggagcgcc tggcgagcga
      541 gcagcagtcc acccgcgagc tgatccgcaa cctcgcgcac gagatcaaga atccgctggg
      601 gggcttgcgc ggctccgcgc agctgctcga gcacgagctc gatcgcgcgg agctgcgcga
      661 gtacacgcag gtgatcatca aggaagccga ccgcctgcag acgctgatgg accggctgct
      721 cacgccgcat cgccagccgc gcgtcgagcg cctgggcatc cacgaggtgc tggagcgggt
      781 gcgcagcctc gtgctggcgg agttcgctcg cgcagccgtg gaaatcgagt gcgactacga
      841 ccccagcctg ccggacctcg agggcgacaa ggagcagctg atccaggcgg tgctcaacgt
      901 ggtgcgcaac gccgcgcagg cggtggccgg gagcggccgg cccggccgca tcaccttgcg
      961 cacgcgggcc atccgccagg tgacgctgct ccggcagcgc cacaggctgg cactagaatt
     1021 gcaagtgatc gacaacgggc ccggcgtccc agaggacatc cgggagaaga ttttcaaccc
     1081 cctggtctcg gggcgggacg gcggcagcgg gctcggacta tcgctcgcgc agactttcgt
     1141 ccactaccac cggggtgtca tcgagtgcga cagccgcccc gggcgcaccg ttttcaggat
     1201 cgtgttgccg ctggcatgaa accggtctgg atcgtcgacg acgaccgctc catccgctgg
     1261 gtgttcgaga aggcgctcgg gcgcgagggc atcgccttca attcgttctc ctcggcggac
     1321 gaggcgctgc acgcattgcg cagcgggccg ccgcaggtcc tggtctcgga catccgcatg
     1381 ccggggccct cgggcctgga gctgctgcag acagtcaagc agcagcaccc cgcggtgccc
     1441 gtgatcgtca tgaccgcgta ctccgacctg gagtcggcgg tcgccgcgtt ccagggcggc
     1501 gccttcgagt acctgccgaa gcccttcgac gtcgaccagg cggtggagct gatccggcgc
     1561 gcgctcgacg agagccagcg cgacgagcgc gcggcggagc cgcccgcgga ggcgccggag
     1621 atcctcggcc aggcgcccgc gatgcaggaa gtctttcgcg ccatcggccg gttgtcgcaa
     1681 tccagcgcga cggtgctgat cacgggcgag tccggctcgg gcaaggagct cgtggcgcgc
     1741 gcgctgcaca ggcatagtgc acgcgccgcg cggccgttcg tggcgatcaa taccgcggcc
     1801 atgcccaagg acctgctgga atcggagctc ttcggccacg agcgcggcgc cttcacgggc
     1861 gcgcagcagc tgcgccgcgg ccgcttcgag caggccgagg gcggcacgct gttcctggac
     1921 gagatcggcg acatgccggc cgacctgcag acgcgcctgc tgcgcgtgct gtccgaaggg
     1981 agcttctacc gcgtgggcgg ccaccagccc atcaagacca acgtgcgcgt catcgcggcc
     2041 acgcaccaga acctggaagc gcgcgtgcgt gaaggcg
//
LOCUS       DASPGE010000089         4305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_848668, whole genome shotgun sequence.
ACCESSION   DASPGE010000089 DASPGE010000000
VERSION     DASPGE010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848668"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1412
                     /gene="leuC"
                     /locus_tag="VD791_02800"
     CDS             3..1412
                     /gene="leuC"
                     /locus_tag="VD791_02800"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HXV06931.1"
                     /translation="MNAPQTLYDKLWSSHVVREEADGTALVYIDRHLVHEVTSPQAFE
                     GLKLAGRRPWRVDSIVATADHNTPTRDWDQGIRDPISRTQVETLDANIRQYGAKAYFP
                     FLDGRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFACLAFGIGSSEVEHVLATQC
                     LLLKKAKRMLVEVEGTLGRGVSAKDIVLAVIGRIGTAGGTGHAIEFAGSAVRALSMEG
                     RMTLCNMAIEAGARAGMVAVDDTTIAYLRGRQFAPKGDLWERAVAVWRTLRTDPGAQF
                     DRVVRLDAAQIKPQVTWGTSPEMVTTVDGRVPDPAQIEDPVKRDAVERALQYMGLQPN
                     TPIGEIRIDKVFIGSCTNSRIEDLRNAAEVVKGRRIASNVKLAMVVPGSGPVKRQAEA
                     EGLDRVFRDAGFEWREPGCSMCLAMNDDRLEPGERCASTSNRNFEGRQGAGGRTHLVS
                     PAMAAAAAIAGRFVDVRSS"
     gene            1426..2064
                     /gene="leuD"
                     /locus_tag="VD791_02805"
     CDS             1426..2064
                     /gene="leuD"
                     /locus_tag="VD791_02805"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HXV06932.1"
                     /translation="MEPFVRHTGLVAPLDRANVDTDAIIPKQFLKSIKRSGFGPNLFD
                     AWRYLDHGEPGMDARRRALNPNFVLNQQRYHGASILLARENFGCGSSREHAPWALLDF
                     GFRAVIAPSYADIFFSNCFKNGLLPIVLSAGSVDLLFHEVLAEPGYRLTVDLPAQAVS
                     TPSGKTHAFDIDPFRKHCLVNGLDEIALTLQHVDEIKAFEARRRAEQPWLFG"
     gene            2069..3142
                     /gene="leuB"
                     /locus_tag="VD791_02810"
     CDS             2069..3142
                     /gene="leuB"
                     /locus_tag="VD791_02810"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HXV06933.1"
                     /translation="MKVAVLPGDGIGPEIMAQALKVLDALAGHGLKMEMEQAPVGGAG
                     YEAAGDPLPPATLELARKADAVLFGAVGGPQYDALPRAKRPEQGILGLRKALGLFANL
                     RPAVLYPELAGASALKPEVVAGLDIMILRELTGDIYFGQPRGRQRTGDGEREGFDTMR
                     YRESEIARIARVGFETALQRSRRLCSVDKANVLDTSILWREVVSEVARDYPQVELTHM
                     YVDNAAMQLVRAPRQFDVIVTGNMFGDILSDEASMLTGSIGMLPSAALDADGKGLYEP
                     IHGSAPDIAGKGVANPLATILSAAMMLRYTFKRADAAQRIEGAVRQVLRQGLRTADIW
                     QEGARKVGTEEMGDAVVSALAHR"
     gene            3276..>4305
                     /gene="asd"
                     /locus_tag="VD791_02815"
     CDS             3276..>4305
                     /gene="asd"
                     /locus_tag="VD791_02815"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012338485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HXV06934.1"
                     /translation="MRVGFIGWRGMVGSVLMQRMREERDFEHIEPMFFSTSQAGGKGP
                     AIGREVPPLKDARDVKALAQHEVLISCQGGDYTGEIVPRLRASGWKGYWIDADKALRM
                     NDDAVIILDPVNLHVIQDALARGIKDYIGGNCTVSLMLMAMHGLLKAGLVEWITAMTY
                     QAASGAGAQNMRELVAQMGTVREAAGGLVDDPASSILDIDRAVAGALRSPGLPREHFG
                     FALAGSLLPWIDKDLGNGQSREEWKAQAEGNKILGRAANPIPMDGICVRIGTMRCHAQ
                     ALTVKLTRDVPLSDVESLLAGANAWVKVVPNEREASLRELTPAQVTGTLNVPVGRLRK
                     LPMGGQYLA"
BASE COUNT          701 a         1442 c         1525 g          637 t
ORIGIN      
        1 ccatgaacgc accgcaaacc ctgtacgaca agctctggtc cagccatgta gtgcgcgagg
       61 aggccgacgg caccgcgctc gtctacatcg accgtcacct ggtgcacgag gtgaccagcc
      121 cgcaggcctt cgaggggctg aagctcgccg ggcgccgacc gtggcgcgtc gactcgatcg
      181 tcgccaccgc cgaccacaac acgcctacgc gcgactggga ccagggcatc cgcgatccga
      241 tctcgcgcac gcaggtcgag acgctggacg ccaacatccg gcagtacggc gccaaggcct
      301 actttccttt cctggatggg cgccaaggca tcgtccacgt gatcggtccc gagcaggggg
      361 ccaccctgcc cggcatgacg gtggtgtgtg gcgattcgca taccagcacg cacggcgcct
      421 tcgcctgcct ggcgttcggc atcggcagct ccgaggtcga gcacgtgctc gccacccagt
      481 gcctgctgct gaagaaggcc aagcgcatgc tggtggaggt cgaaggcacg ctgggccgcg
      541 gcgtgagcgc gaaggatatc gtgctggcgg tgatcggcag gatcggcacc gccggcggca
      601 ccggccacgc catcgagttc gccggcagcg ccgtgcgcgc cctgtcgatg gaagggcgca
      661 tgaccctgtg caacatggcg atcgaggccg gtgcacgcgc cggcatggtg gccgtggacg
      721 acaccaccat cgcttacctg cgcggccgcc agttcgcgcc gaagggcgat ctgtgggagc
      781 gggcggtggc ggtctggcgc acgctgagaa ccgatcccgg cgcgcagttc gatcgcgtgg
      841 tgcggctcga tgccgcgcag atcaagccgc aggtcacctg gggcacctcg cccgagatgg
      901 tgaccaccgt ggacggccgc gtgcccgatc cggcgcagat cgaggatccg gtgaagcgcg
      961 acgccgtcga acgcgcgctg cagtacatgg gactgcagcc gaacacgccg atcggcgaga
     1021 tccgcatcga caaggtgttc atcggctcct gcaccaactc gcgcatcgag gacctgcgca
     1081 acgccgccga ggtggtgaag ggccgccgca tcgcgtccaa cgtcaagctg gcgatggtgg
     1141 tgcccggctc cgggccggtg aagcgccagg cggaggccga ggggctggac cgcgtgttcc
     1201 gcgacgccgg cttcgagtgg cgcgagcccg gctgctccat gtgcctggcg atgaacgacg
     1261 accggctcga gccgggcgaa cgctgcgcat ccacctcgaa ccgcaacttc gagggacgtc
     1321 agggtgcggg cgggcgcacg cacctggtga gcccggcaat ggccgcggcg gccgccatcg
     1381 ccggccggtt cgtggacgtg cgctcgagct agagaggggc agccgatgga accgttcgta
     1441 cgacacaccg ggctggtggc cccgctcgac cgcgccaacg tcgacaccga cgccatcatc
     1501 cccaagcagt tcctgaagtc gatcaagcgc agcggcttcg gcccgaacct gttcgatgca
     1561 tggcgctatc tcgatcacgg cgagcccggc atggacgcgc gccgccgcgc gctcaacccg
     1621 aatttcgtgc tcaaccagca gcgctaccat ggcgcttcga tcctgctcgc gcgcgagaac
     1681 ttcggctgcg gctcgtcgcg cgagcacgcc ccctgggcgc tgctcgactt cggctttcgc
     1741 gccgtcatcg cgcccagcta cgccgacatc ttcttcagca actgcttcaa gaacggcctg
     1801 ctcccgatcg tgctgtccgc cggcagcgtc gacctgctgt tccacgaggt gctggccgag
     1861 ccgggctatc gcctgaccgt ggacctgccc gcgcaggcgg tgagcacgcc ctccggcaag
     1921 acccatgctt tcgacatcga cccgttccgc aagcactgcc tggtgaacgg cctcgacgag
     1981 atcgcgctca cgctgcagca cgtggacgag atcaaggcct tcgaggccag gcgccgcgcc
     2041 gagcagccgt ggctgttcgg ttgacgcgat gaaagtcgcg gtcctgcccg gagacggcat
     2101 cggcccggag atcatggccc aggcgctgaa ggtgctcgac gcgctggccg gccacggtct
     2161 gaagatggag atggagcagg cgccggtggg cggcgccggc tacgaggccg ccggcgaccc
     2221 gctgccgccc gccacgctgg agctggcgcg caaggcggac gccgtgctgt tcggcgccgt
     2281 gggtggcccg cagtacgacg cgctgccgcg ggccaagcgg ccggagcagg gcatcctggg
     2341 cttgcgcaag gcgctcgggt tgttcgccaa cctgcgcccg gcggtgctct atcccgaact
     2401 ggcgggcgcc tccgcgctca agccggaggt ggtggccggg ctggacatca tgatcctgcg
     2461 tgagctgacc ggcgacatct acttcggcca gccacgcggc aggcaacgca ccggcgacgg
     2521 ggagcgcgag ggattcgaca cgatgcgcta ccgggagtcg gagatcgcac gcatcgcgcg
     2581 cgtcgggttc gagacggcgc tgcagcgcag ccgccggctg tgctcggtgg acaaggccaa
     2641 cgtgctggac accagcatcc tgtggcgcga ggtggtgagc gaggtggccc gggactatcc
     2701 gcaggtcgag ctcacgcaca tgtacgtgga caacgcggcg atgcagctgg tgcgcgcgcc
     2761 caggcagttc gacgtgatcg tgaccggcaa catgttcggc gacatcctgt ccgacgaggc
     2821 ctccatgctc accgggtcga tcggcatgct gccctcggcg gcgctggacg cggacggcaa
     2881 gggcctgtac gagccgatcc acggctccgc ccccgacatc gccggcaaag gcgtcgccaa
     2941 tccgctcgcc accatcctat cggccgcgat gatgctgcgc tacacgttca agcgcgcgga
     3001 cgcggcgcag cgcatcgagg gtgcggtgcg ccaagtgctg cgccaggggt tgcgtaccgc
     3061 cgacatctgg caggagggcg cgcgcaaggt cggcaccgag gagatgggcg acgcggtggt
     3121 ctccgccctc gcgcatcgct agcgttgctt gaaccgcatt caccacgaag agcgcgaagg
     3181 aacgacagag agcatgtttt ccttcgcgct cttggtgtat cttcgtggcc ttcgcggttg
     3241 acgcgtttgg ttttgaagca aggttgagca aaatgatgcg agtgggtttc atcggctggc
     3301 gcggcatggt gggctcggtg ctgatgcagc gcatgcggga ggagcgcgac ttcgagcaca
     3361 tcgagccgat gtttttcagc acctcccagg ccggcggcaa gggcccggcc atcgggcggg
     3421 aggtgccgcc gctcaaggat gcgcgcgacg tgaaggcact cgcgcagcac gaggtgctga
     3481 tctcctgcca gggcggggac tacaccggcg agatcgtgcc gcgcttgcgc gcctcgggct
     3541 ggaaggggta ctggatcgat gccgacaagg cgctgcgcat gaacgacgac gcggtcatca
     3601 tcctcgaccc ggtcaacctg catgtcatcc aggacgcgct cgcgcgcggc atcaaggact
     3661 acatcggcgg caactgcacc gtgagcctga tgctgatggc catgcatggc ctgctcaagg
     3721 ccggcctggt cgagtggatc accgccatga cctatcaggc ggcctccggc gcgggcgcgc
     3781 agaacatgcg cgaactggtg gcgcagatgg gcacggtgcg tgaggcggcg ggcgggctgg
     3841 tggacgatcc cgcgtcctcg atcctcgaca tcgaccgggc ggtggcgggc gcgctgcgat
     3901 ccccggggct gccgcgcgag catttcggct tcgcgctggc cggcagtctg ctgccgtgga
     3961 tcgacaagga cctgggcaac ggccagagcc gcgaggagtg gaaggcgcaa gccgagggca
     4021 acaagatcct gggacgggcc gccaacccga tcccgatgga cggcatctgc gtgcgcatcg
     4081 gcaccatgcg ctgtcacgcc caggcgctca ccgtcaagct tacccgcgac gtgccgttgt
     4141 ccgatgtcga aagcctgctg gcgggcgcga acgcctgggt caaagtggtg cccaacgaac
     4201 gtgaggcgag cttgcgcgag ctcacgccgg cgcaggtgac cggtacgctg aacgtaccgg
     4261 tgggccggct gcgcaagctg ccgatgggcg gccaatatct cgcgg
//
LOCUS       DASPGE010000090         9905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_28149, whole genome shotgun sequence.
ACCESSION   DASPGE010000090 DASPGE010000000
VERSION     DASPGE010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9905
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28149"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /gene="pyrH"
                     /locus_tag="VD791_02820"
     CDS             <1..540
                     /gene="pyrH"
                     /locus_tag="VD791_02820"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HXV06935.1"
                     /translation="GVALGAAGMDRATADYMGMLATVMNALALQDAMRQVGLLSRVQS
                     ALNIEQVAEPYIRGKAMRYLEEGKVVIFAAGTGNPFFTTDTAAALRGMEMNVDIVIKA
                     TKVDGVYTDDPKANPGAMRYKRLTFDEAIVKNLKVMDATALTLCRDQKLPISVFSIFK
                     PGALKRLVLGEDEGTLVHS"
     gene            602..1162
                     /gene="frr"
                     /locus_tag="VD791_02825"
     CDS             602..1162
                     /gene="frr"
                     /locus_tag="VD791_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HXV06936.1"
                     /translation="MMIADVKKSAEQKMQKSVEALKADLGKVRTGRAHTGLLDHIVID
                     YYGTPTPINQVANVTLLDARTIGVSPWEKRLASAIEKAIRDSDLGLNPATVGDLVRVP
                     MPALTEERRKDLIKVVRHEAENARVAVRNIRRDANTHLKDLLKQKKVPEDEERRAQED
                     IQKLTDRYIAEVDKLLHQKEADLMAV"
     gene            1188..1976
                     /gene="uppS"
                     /locus_tag="VD791_02830"
     CDS             1188..1976
                     /gene="uppS"
                     /locus_tag="VD791_02830"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HXV06937.1"
                     /translation="MTVFDSSTIGIPATADVPTHIAIIMDGNGRWAKQRLLPRVAGHR
                     RGLETVRSVTRFCIEAGVEYLTLFAFSSENWRRPAEEVSFLMQLFMRALESEVVRLHE
                     NNIRLKVVGDLSSFESRLVGLVQEAEALTAANTRLTLTIAANYGGRWDIMQAVNRLVR
                     EHAERSAGFNEAHLAPYLALSYAPEPDLFVRTGGEQRVSNFCLWQLAYTELYFTDVLW
                     PDFDRAAFDAALASYRRRERRFGRTSEQMAQQRTPPAALPAEPR"
     gene            1978..2805
                     /locus_tag="VD791_02835"
     CDS             1978..2805
                     /locus_tag="VD791_02835"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HXV06938.1"
                     /translation="MLWQRILTAGVLLPLFLAALFLLPNLYWALLLAALLAVAAREWS
                     RLAGFGAAGQATYALALLSAALAIVALDHDDGAAHAFIYSAPGKLLYALCAAFWLAIV
                     PAWLYYRWVVRSRPLLAVVGVVVLLPVWHALAWLQLTPERLLVALAVVWIADTAAYFA
                     GRAFGRHKLAPSISPGKTWEGVAGALAAVCSGWVAFALLAPSFRAQLASGLLLAALLT
                     AMSVQGDLFESWMKRQAGRKDSGALLPGHGGLLDRVDALTSTLPLAALYFAYPLLRS"
     gene            2816..4027
                     /gene="ispC"
                     /locus_tag="VD791_02840"
     CDS             2816..4027
                     /gene="ispC"
                     /locus_tag="VD791_02840"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HXV06939.1"
                     /translation="MRTSSVPRTLTILGATGSIGASTLDVLARHPERFRVAALTAHRQ
                     TAAMVQVCLRFRPRYAVMTEEAAAAALSTELRRAGLATEVLAGAEGLTAVATLPEVDS
                     VMAAIVGAAGLRPTLAAARAGKRVLLANKEALVMAGRLFMDAVREGGARLLPIDSEHN
                     AILQSMPAGFDGDLHAAGVRRILLTASGGPFRNTPQGRLAAVTPAQACAHPNWVMGRK
                     ISVDSATMMNKGLEVIEAHWLFGAPAEAIEVVIHPQSVIHSMVEYVDGSVLAQLGNPD
                     MRTPIAYALGFPERIESGARALNFFELGALNFERPDLARFPCLALAYQALRAGGSAPA
                     VLNAANEVAVEQFLKGELAFDRIAHLIEDVLSRVDGMPVDSLEQVLDADRAAREAAYR
                     YGATEKRDRTA"
     gene            4062..5414
                     /gene="rseP"
                     /locus_tag="VD791_02845"
     CDS             4062..5414
                     /gene="rseP"
                     /locus_tag="VD791_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HXV06940.1"
                     /translation="MTLATTLLAFIVALGLLIVVHELGHYMVARWCGVKVLRFSVGFG
                     TPLWLRRFGPDQTEWVVAAFPMGGFVKMLDEREGEVAPHERERAFNRQSVYRRFAIVL
                     AGPLANFLLAIAVYWLLFMHGVPGFKPILGAPPPATAAAQAQIAAGETILRVDDQEVR
                     TWQDVRWALLERAVERQTVTLEVQNEKAEIAFRKLDLSGIEAADLDGDFLAKIGLTRL
                     PPPPVVGEVTAGKPGEKAGLKPGDRILAVDGKPLATWDDLVAAVAPKGGAPLTLQLRR
                     GADELTVQVTPETVQQDGKSIGRIWVAPDRAALERQMVEVRYGPLAALGQGAVRTWEV
                     SILSLRMLGRMIVGHVSLKNLSGPITIADYAGQSARMGWVAYASFVALISISLGVLNL
                     LPVPLLDGGHLMYYFIEIIKGRPVSERTLEIGQRIGMVVLFTLMAFALFNDIHRLVGG
                     "
     gene            5430..7697
                     /gene="bamA"
                     /locus_tag="VD791_02850"
     CDS             5430..7697
                     /gene="bamA"
                     /locus_tag="VD791_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HXV06941.1"
                     /translation="MKLARLLLFLSGLSTISASAFEPFEVKDIRVEGIQRTEAGTVFS
                     YLPIRVGETLTEERAAAALRALYATGFFKDVQLEAEGDVLVVVVQERPAIGQIEFVGQ
                     KEFDRDRLKAALKDIGLAEGRVFDRSLLQRAEQELKSQYLGRGKYSAQITTTVSPLER
                     NRVAIQFSIVEGPVAKIREINFVGNEAVKESQLRDLMRLDTPGLLSWYSKNDQYSRQK
                     LEADLEAIRSSYLNQGYLESAIDSTQVSISPDKKDIYLTVNLTEGKKFTVSEIRFAGE
                     LIAPEAELRKLLRLKAGEQFSRDKLAESTKLIVDRLGDDGYAFANVNPVPEIDQDKAT
                     VAFTLYVDPGRRVYIRRINISGNATTRDEVIRRELRQLEGAWYSTQKLQRSKQRVDKL
                     GYFSEVTLDTVGVPGVPDQVDINLKVVERPTGNLLFGVGYSSAEGVIFSGAIAQNNLF
                     GTGNALSLQVNTGNVNTVYALSFTRPYFTDDGMGLGLDLYRRDVDASSLSVSTYSTST
                     LGTGIRFSVPITETDTINYGLAGESTEIELGEAPAQRYVDFVDAFGTENTALVTTAGW
                     ARDKRDSVIFTRRGTVQKISVEVAVPPAELRYYRTQYELQWFLPIGRDNVLQLSGRVG
                     YADGYNDKPLPFYKNFYLGGVSTLRGYETASVGPEDENGDAIGGQALLVSSAELFFPF
                     PGLEKDRSVRLSLFLDAGALDDTFSFTEWRASTGLALSWFSPVGPLKISYGHALNDEP
                     GDELQPFQFSIGTVF"
     gene            7740..8222
                     /locus_tag="VD791_02855"
     CDS             7740..8222
                     /locus_tag="VD791_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HXV06942.1"
                     /translation="MTLATLLLCTAGFAHANDIKVGFVSTERLFREAAPAVRALKKLE
                     KEFAPRDAELQKLGKQAKDLQAQLEKEGMTMSEGDRRGKEQDLARMNRELQRMQRELR
                     EDLNLRKNEELAQVLERANKVIQEIAEKEKYDLILQEAVYRSPRIDITDKVLKALSDK
                     "
     gene            8233..9300
                     /gene="lpxD"
                     /locus_tag="VD791_02860"
     CDS             8233..9300
                     /gene="lpxD"
                     /locus_tag="VD791_02860"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HXV06943.1"
                     /translation="MSDSSSSTAPRRAVTLSQIVERFGGELLGEGEVRVDQVATLESA
                     TGGQISFFVNPRYRRQLEQTQAAAVIVKREAVGLIERPLIVCDNPYAYFGRVSDFLNP
                     PEAVIPGVHPTAAIDADAQVHAGACIGPLASVAAGAVIGERTLLGPGSHVGARARIGA
                     DCRIHANVSIYHDCSVGARAILHSGAVIGADGFGVAMHGGRWLKIPQIGRVIVGDDVE
                     VGANTTIDRGALDDTVIEDGVKLDNQIQIGHNCRIGAHTAIAGCVGIAGSTRIGRYCR
                     IGGGAMIGGHLEIADGVEIGAATAVGKSISRPGTYSGALPMSPHPEWLKNAAHIRHLD
                     RMAHRIRELESRLADLERKQR"
     gene            9306..9740
                     /gene="fabZ"
                     /locus_tag="VD791_02865"
     CDS             9306..9740
                     /gene="fabZ"
                     /locus_tag="VD791_02865"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HXV06944.1"
                     /translation="MEIREILECLPHRHPFLLLDRVLSCEPGKSIVAIKNVAIGEPFF
                     QGHFPGNPIMPGVMILEAMAQAAAILSFKTLHHSSTRNLLYYFVGIDAARFKKPVVPG
                     DQLVLEVTMLRIVRGIGKFTARARVGEVAVAEAELLCTIKED"
BASE COUNT         1640 a         3284 c         3443 g         1538 t
ORIGIN      
        1 ggcgtggctt tgggagccgc cggcatggat cgtgcgacgg ccgattacat gggcatgctg
       61 gccacggtca tgaacgccct ggcgctgcag gacgccatgc gccaggtcgg gctgctgagc
      121 cgggtgcagt cggcgctgaa catcgagcag gtggccgaac cgtacatccg cggcaaggcg
      181 atgcgctacc tcgaggaagg caaggtcgtg atcttcgccg ccgggaccgg caacccgttc
      241 ttcaccaccg acaccgcggc cgcgctgcgc ggtatggaga tgaacgtgga catcgtgatc
      301 aaggccacca aggtggacgg ggtctacacc gacgatccca aggccaatcc cggggccatg
      361 cgctacaagc gcctcacctt cgacgaggcg atcgtcaaga acctcaaggt gatggacgcc
      421 accgcgctca ccctgtgccg cgatcagaag ctgccgatca gcgtgttcag catcttcaag
      481 ccgggggcgc tcaagcgcct ggtcctgggg gaggacgagg gcacgctggt gcacagctga
      541 cccgccggcc cgggggatca tactcagcaa ggcaccgggg ggtcgcccgt cgataggagg
      601 catcatgatc gccgatgtca agaaatccgc cgagcagaaa atgcagaaga gcgtggaagc
      661 gctcaaggcc gacctcggca aagtgcgcac cggccgcgcc cacaccggtc tgctggacca
      721 tatcgtgatc gactactacg gtaccccgac gccgatcaac caggtggcca acgtcactct
      781 gctggacgca cgcaccatcg gcgtgtcccc ctgggagaag cgcctggcga gcgcgatcga
      841 gaaggcgatc cgcgattccg acctgggcct gaatccggcc accgtgggcg acttggtgcg
      901 cgtgccgatg ccggcgctca ccgaggaacg gcgcaaggac ctgatcaagg tggtgcggca
      961 cgaggccgag aacgcgcgcg tggcggtgcg caacatccgg cgcgacgcga atacccacct
     1021 caaggatctg ctcaagcaga agaaggtgcc cgaggacgag gagcgccgcg cgcaggagga
     1081 catccagaag ctcaccgacc gctacatcgc cgaggtggac aagctgctgc accagaagga
     1141 agccgacctg atggcggtgt gatcggcgca gcccggggat cgctctcgtg accgtcttcg
     1201 acagctccac catcggcatt ccggccaccg ccgacgtgcc cacgcacatc gccatcatca
     1261 tggacggcaa cggccgctgg gcaaaacagc gcctgctgcc gcgcgtggcc ggccatcgcc
     1321 gcgggctgga gacggtgcgc tccgtgaccc gcttctgcat cgaagccggg gtggaatacc
     1381 tcaccctgtt cgccttcagc agcgaaaact ggcgccggcc cgctgaggaa gtgtcgttcc
     1441 tgatgcaact cttcatgcgc gcgctggaga gcgaggtggt gcggctgcac gagaacaaca
     1501 tccggctcaa ggtggtgggc gatctgtcca gcttcgagtc gcgcttggtg ggcctggtgc
     1561 aggaagccga ggcgctcacc gccgccaaca cgcggctcac gctcaccatc gccgccaact
     1621 acggcgggcg ctgggacatc atgcaggccg tcaaccgctt ggtgcgcgag cacgccgagc
     1681 gtagcgccgg cttcaacgag gcgcatctcg ctccctatct ggcgctctcc tacgctcccg
     1741 agcccgatct gttcgtgcgc accggtggcg agcagcgggt cagcaacttc tgcctctggc
     1801 agctcgccta caccgagctg tacttcaccg acgtactgtg gccggacttc gaccgggccg
     1861 ccttcgacgc ggcgctggcc tcctaccgcc ggcgcgagcg ccgcttcggc cgcaccagcg
     1921 agcagatggc ccagcagcgc acccctcccg ccgcgctgcc ggccgagccg cgctagcatg
     1981 ctgtggcagc gcatcctcac ggcgggcgtg ctgctgccgt tgttcctcgc tgcgctgttc
     2041 ctgctgccca atctgtactg ggcgctgctg ctcgccgctc tgctggcggt ggccgcgcgc
     2101 gaatggtcgc gcctggcggg gttcggggcc gctggccagg ccacgtatgc cctggcgctg
     2161 ctctccgcgg cgctggcgat cgtcgccctg gatcacgacg acggcgccgc ccacgccttc
     2221 atctacagcg cgccggggaa gctcctgtac gcgctgtgcg ccgctttctg gctggcgatc
     2281 gtgccggcct ggctgtacta ccgctgggtg gtgcgctccc ggcccctgct cgccgtcgtc
     2341 ggcgtggtag tgctgctgcc ggtctggcat gcgctggcgt ggctgcagct cacgcccgag
     2401 cggctgctgg tggcgctggc cgtggtctgg atcgcggaca ccgccgccta cttcgcgggg
     2461 cgcgccttcg gacggcacaa gctcgcgccc agcatcagcc cgggcaagac gtgggaaggc
     2521 gtggccggcg cgctcgccgc ggtatgcagc ggctgggtgg cgttcgcgct gctggcgccg
     2581 agcttccgcg cgcagctcgc ctccgggctg ttgctcgcgg ccctgctcac cgcgatgagc
     2641 gtccagggcg atctgttcga gtcctggatg aagcgccagg ccggccggaa ggacagcggc
     2701 gcgctgcttc cgggtcacgg cgggctgctc gaccgggtgg acgcgctcac ctccaccctg
     2761 ccgctggcgg ccctatactt cgcctatcct ctgctgcgca gctgacatgc ccgccttgcg
     2821 cacttcctcc gtgcctcgaa cgctgaccat cctgggggcc accggctcga tcggcgcgag
     2881 caccctcgac gtgctggccc ggcatccgga gcgcttccgg gtagcggcgc tcaccgccca
     2941 tcgtcagacg gcggcgatgg tgcaggtctg cctgcgtttc cggccgcgct acgcggtgat
     3001 gaccgaagag gcggcggccg cggcgctgag taccgagctg cgccgcgcgg ggctggcgac
     3061 cgaggtgctg gccggtgccg agggtctgac cgcggtggcg acgctgccgg aggtggatag
     3121 cgtcatggcc gcgatcgtgg gcgcggcagg cctgcggccg acgctggcgg cggcgcgcgc
     3181 cggcaagcgc gtgctgctcg ccaacaagga ggccctggtg atggcgggcc ggctgttcat
     3241 ggatgcggtg cgcgagggcg gagcgcgcct gctgccgatc gacagcgagc acaacgccat
     3301 cctgcaatcc atgccggcgg gtttcgacgg cgacctgcac gcggccggcg tgcggcgcat
     3361 cctgctcacc gcctccggcg gtccgttccg caacacgccg cagggccggc tcgcggcggt
     3421 cacgccggcg caggcctgcg cccaccccaa ctgggtgatg ggccgcaaga tctcggtgga
     3481 ctcggccacc atgatgaaca aggggctgga ggtgatcgag gcgcactggc tgttcggcgc
     3541 gccggccgag gcgatcgagg tggtgatcca cccgcagagc gtgatccact cgatggtcga
     3601 atacgtcgac ggctcggtgc tggcgcagct gggcaatccc gacatgcgca cgcccatcgc
     3661 gtacgcgctc ggcttccccg agcgcatcga atcgggcgca cgcgcattga actttttcga
     3721 gctgggcgcg ctcaacttcg agcgtcccga cctcgcccgt ttcccgtgcc tggcgctcgc
     3781 ctaccaggcc ctgcgcgcgg gcggcagcgc gccggccgtg ctgaacgccg ccaacgaagt
     3841 ggccgtagag cagttcctga aaggagagct ggcgttcgat cgcatcgcgc acctcatcga
     3901 ggacgtgctg tcgcgggtgg acggaatgcc ggtcgacagc ctggagcagg tgctcgacgc
     3961 ggatcgcgcg gcgcgggagg cggcataccg gtacggcgcg accgagaagc gcgaccgaac
     4021 ggcgtgaccg gtcacgctga cctccagaaa gcggagcaac catgacatta gccaccacac
     4081 tgcttgcgtt catcgtcgca ctgggcctgc tgatcgtggt gcacgagctg ggccactaca
     4141 tggtggcgcg ctggtgcggc gtgaaggtgc tgcgcttctc ggtgggattc ggcacgccgt
     4201 tgtggctgcg ccgcttcggg ccggaccaga ccgagtgggt cgtggccgca ttcccgatgg
     4261 gcggcttcgt gaagatgctg gacgagcgcg agggcgaagt cgccccgcac gagcgcgagc
     4321 gcgccttcaa ccggcagtcg gtgtaccgcc gcttcgccat cgtgctggcc gggccgctcg
     4381 ccaacttcct gctcgcgatc gccgtgtact ggctgctgtt catgcacggg gtgccgggct
     4441 tcaagccgat cctcggtgcg ccaccgcccg ccacggcggc agcccaggcg cagatcgctg
     4501 ccggcgagac catcctgcgg gtggacgatc aggaagtccg cacctggcag gacgtgcgct
     4561 gggcgctgct cgagcgggcc gtggagcgcc agacggtgac cctcgaggtg cagaacgaga
     4621 aggccgagat cgcatttcgc aagctcgacc tgtcggggat cgaagccgct gatctggacg
     4681 gcgatttcct ggccaagatc ggcctcaccc ggctgccgcc accgcccgtc gtcggcgagg
     4741 tgactgcagg caagcccggc gagaaagcgg ggctgaagcc cggcgatcgc atcctggcgg
     4801 tcgacggcaa gccgctggcg acctgggacg atctggtggc ggcggtggct ccgaagggcg
     4861 gcgcgccgct gacgctgcag ttgcgccggg gcgcggacga gctcaccgtg caggtgacgc
     4921 cggagacggt gcagcaggac ggcaagtcga tcggccgcat ctgggtcgca ccggatcgcg
     4981 cggcgctcga gcgccagatg gtggaagtgc gctacggccc gttggcggcg ctgggccagg
     5041 gagcggtgcg cacctgggag gtgtcgatcc tgagcctgcg catgctcggc cggatgatcg
     5101 tgggccacgt gtcgctgaag aatctgagcg gtccgatcac catcgccgac tacgcaggcc
     5161 agtcggcgcg catgggatgg gtggcctacg cgagcttcgt ggcgctgatc agcatcagcc
     5221 tgggagtgct caacctgctg ccggtgccct tattggacgg cgggcacttg atgtattatt
     5281 tcattgaaat catcaaggga cgaccggtgt ccgagcgcac cctggagatc gggcagcgca
     5341 tcgggatggt ggtactgttc accctgatgg ccttcgcgct cttcaacgac attcaccgct
     5401 tggtcggcgg ctgacaacaa caccactgga tgaagctggc gcggctgctg ctattcctat
     5461 cgggcctttc caccatctcc gcatccgcct tcgaaccgtt cgaggtcaag gatatccgcg
     5521 tcgagggcat ccagcgcacc gaggccggca ccgtcttcag ctacctgccg atccgggtcg
     5581 gcgagaccct taccgaggag cgggccgccg ccgcactgcg cgcgctgtac gccaccggtt
     5641 tcttcaagga cgtgcagctg gaggccgagg gcgacgtgct ggtggtcgtc gtgcaggagc
     5701 gaccggcgat cggccagatc gagttcgtcg gccagaagga gttcgaccgg gacaggctca
     5761 aggcggcgct caaggacatc gggctggccg aggggcgcgt gttcgaccgc agcctgctgc
     5821 agcgggcgga gcaggagctg aagagccagt acctcgggcg cgggaagtac tccgcgcaga
     5881 tcaccaccac ggtgtcgccg ctggagcgca accgcgtcgc gatccagttc agcatcgtgg
     5941 agggtccggt ggccaagatc cgcgagatca acttcgtcgg caacgaggcg gtcaaggagt
     6001 cgcaactgcg cgacctcatg cgcctggata ccccggggct gctcagctgg tacagcaaga
     6061 acgaccagta ctcgcggcag aagctggagg ccgatctgga ggcgatccgc tcgtcctatc
     6121 tgaaccaggg ctatctcgag tcggcgatcg actccacgca ggtgtcgatc tcgccggaca
     6181 agaaggacat ctatctcacg gtcaacctca ccgaaggcaa gaaattcacc gtctccgaga
     6241 tccggttcgc gggtgagctg atcgcacccg aggcggagct gcgcaagctg ctcaggctca
     6301 aggccggcga acagttttcg cgcgacaagc tggccgagtc caccaagctg atcgtcgatc
     6361 ggctcggaga cgacggctac gcctttgcca acgtgaaccc ggtgccggag atcgaccagg
     6421 acaaggcgac ggttgcattc accctgtacg tggaccccgg ccggcgcgtg tacatccggc
     6481 gcatcaacat ctcgggcaac gccaccacgc gcgacgaggt gatccgccgc gagctgcgac
     6541 agctcgaagg cgcctggtac tcgacgcaga agctgcagcg ctccaagcag cgcgtggaca
     6601 agctcggtta cttctcggaa gttaccctgg acacggtcgg ggtgccgggc gtgccggacc
     6661 aggtggacat caatctgaaa gtagtggaac ggcccaccgg caacctgctg ttcggggtcg
     6721 gctattccag cgcggaaggc gtgatcttca gcggcgcgat cgcgcagaac aacctgttcg
     6781 gcaccggcaa cgcgctgtcg ctgcaagtga acaccggcaa cgtgaacacc gtgtacgcgc
     6841 tgtcgttcac gcgcccgtac ttcaccgacg acggaatggg cttgggtctg gacctgtacc
     6901 gtcgcgacgt ggacgcgagc agcctgtcgg tgtccaccta cagcaccagc accttgggga
     6961 ccgggatccg cttctcggtg ccgatcaccg agaccgacac catcaactac ggcctggccg
     7021 gtgagagcac ggagatcgaa ctgggcgagg cccccgcgca gcgctacgtc gacttcgtgg
     7081 acgcgttcgg gacggagaac acggcgctgg tgaccaccgc cggctgggcg cgggacaagc
     7141 gcgacagcgt catcttcacg cgccgcggaa cggtgcagaa gatctcggtc gaagtggcgg
     7201 tgccgccggc ggagctgcgc tactatcgca cccagtacga gctgcagtgg ttcctgccga
     7261 tcggccgcga caacgtgttg cagctctccg gacgcgtcgg ctatgccgac ggttacaacg
     7321 acaagccgct cccgttctac aagaacttct acctgggcgg cgtgagcacg ttgcgcggct
     7381 acgagaccgc ctcggtcggc cccgaggacg agaacggcga cgccatcggc ggccaggccc
     7441 tgctggtgag cagcgccgag ttgttcttcc ccttccccgg gctggagaag gaccgctcgg
     7501 tgcgcctgtc gctgttcctt gacgccggcg cgctggatga tacattcagc tttaccgaat
     7561 ggcgcgcctc caccggcctg gcgctgagct ggttctcgcc ggtgggtccg ttgaagatat
     7621 cctacggcca cgccttgaac gacgagccgg gggacgagct gcagccgttc cagttctcga
     7681 tcggcacggt gttctgacgt gccgcagcga agaggagatt tcagttgaat agagttcgaa
     7741 tgacgctcgc gacgctgctg ctgtgcacgg cagggttcgc ccacgccaac gacatcaagg
     7801 tcgggttcgt cagcaccgag cgcctgttcc gcgaggcggc gccggcggtg cgggcgctga
     7861 agaagctgga gaaggaattc gcgccccgcg acgccgagct gcagaagctc ggcaagcagg
     7921 ccaaggacct gcaggctcag ctcgagaagg aaggcatgac gatgagcgag ggcgaccgcc
     7981 gcggcaagga acaggatctg gcgcgcatga accgcgagct gcagcgcatg cagcgcgaat
     8041 tgcgcgagga cctgaacctg cgcaagaacg aggagctagc gcaggtgctg gagcgcgcca
     8101 acaaggtgat ccaggagatc gcggagaagg agaagtacga cctgatcctg caggaggcgg
     8161 tgtaccgcag cccgcgcatc gacatcaccg acaaggtgct caaggcgctc tcggacaagt
     8221 agccgattcg ccgtgtccga ctccagctcc agcaccgctc cgcggcgggc cgtcaccctg
     8281 agccagatcg tcgagcgctt cggcggcgag ctgctgggcg agggcgaggt gcgggtggac
     8341 caggtcgcca cgctggagag cgcgaccggc ggccagatct ccttcttcgt caatccgcgc
     8401 taccggcgcc agctcgagca aacgcaggcg gcggcggtga tcgtgaagcg ggaggcggtc
     8461 ggcctgatcg agcgtccgct catcgtttgc gacaacccct acgcctattt cgggcgcgtg
     8521 tccgactttc tcaacccgcc ggaggccgtg atccccggag tgcatcccac ggcggcgatc
     8581 gatgctgacg cgcaagtcca cgccggcgcc tgcatcgggc cgttggcatc ggtggcggcg
     8641 ggcgccgtga tcggagagcg cacgctcctg gggccgggca gccatgtcgg cgcgcgcgcg
     8701 cgcatcggcg ccgattgccg catccacgcc aacgtctcca tctatcacga ttgcagcgtc
     8761 ggcgcgcgtg ctattctgca ctccggggcg gtgatcggcg cggacgggtt cggcgtggcg
     8821 atgcacggcg gacgctggct gaagatcccg cagatcgggc gggtgatcgt cggcgacgac
     8881 gtggaggtcg gcgccaacac caccatcgac cgcggcgcgc tcgacgacac cgtgatcgaa
     8941 gacggggtga agctggacaa ccagatccag atcggacaca actgccgcat cggcgcacac
     9001 accgccatcg ccggctgcgt cggcatcgcg ggcagcaccc gcatcgggcg ctattgccgc
     9061 atcggcggcg gtgcgatgat cggcggccat ctggagatcg ccgacggcgt ggagatcggt
     9121 gccgccaccg ccgtgggcaa gtcgatttcc agacccggca cctatagcgg agcgttgccg
     9181 atgtcgccgc atcccgagtg gctgaagaac gcggcccaca tccggcacct ggaccggatg
     9241 gcgcaccgca tccgcgagct cgaaagccgg ctcgccgacc tggaaaggaa gcagcgttga
     9301 acagcatgga gatcagggag atcctagagt gcctgccgca caggcacccg ttccttctgc
     9361 tcgatcgcgt cctgtcgtgc gagcccggca agagcatcgt cgcgatcaag aacgtggcca
     9421 tcggcgaacc gttcttccag ggtcactttc ccggcaaccc gatcatgccc ggcgtgatga
     9481 tcctcgaggc gatggcgcag gcggcggcga tcctgtcgtt caagacgctg caccacagca
     9541 gcacgcgcaa cctgttgtac tacttcgtcg gcatcgacgc cgcccgcttc aagaagccgg
     9601 tggtgccggg cgaccagctg gtgctggagg tgacgatgct gcgcattgtg cgcggcatcg
     9661 gcaagttcac cgcccgggcg cgggtgggcg aagtcgcggt ggccgaggcc gagctgctgt
     9721 gcaccatcaa ggaggactga ttggatcccc ggccgagcgc cggagccgcc gatgacggcg
     9781 ttgtctgacc cgccggcgat ccaccccacg gccgtggtcg accccggtgc gcgccttgcc
     9841 gaaggcgtcc gggtgggggc gttctgcgtg atcgacggac acgtggagct gggtgccggc
     9901 acgct
//
LOCUS       DASPGE010000091         2758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1550802, whole genome shotgun sequence.
ACCESSION   DASPGE010000091 DASPGE010000000
VERSION     DASPGE010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550802"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="VD791_02870"
     CDS             complement(<1..1047)
                     /locus_tag="VD791_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXV06945.1"
                     /translation="MKRRSFLKTAAAGMATGAVAAPAIVHAQPSIQWRLAASWPKSLD
                     TLFGGAELVARRVSEVTEGKFQIRVFAGGEIVPPLQVLDAVQAGTVEMGHTASYYYFG
                     KDPAFSFGTAVPFGLNARQNFAWWFFGGGAQAMAPLYKDYGCIAIPAGNTGCQMGGWF
                     RKEIKSVADMKGLKFRMGGMAGLVLAKLGVVPQMISGADIYPSLEKGTIDGAEWVGPY
                     DDEKLGFQKVAKFYYYPGWWEAGPIGLMLVNEKKYNELPKAYQSALHAACGEACVWMP
                     AKYDAENPQSLRKLIASGTKLRPFPRSVMEACEKATYELYAELMEKSAHWKRIYPAWK
                     KFRDEQFLWFRVAEA"
     gene            complement(1119..1886)
                     /locus_tag="VD791_02875"
     CDS             complement(1119..1886)
                     /locus_tag="VD791_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXV06946.1"
                     /translation="MSAHVLTETRERVARIELKRPDKKNALTVEMYQAMADALRAVEA
                     DPQVRVGLIHGQPDCFSAGNDLKDFLERPPHAERSPVFQFLQAISTAAKPLIAAVGGP
                     AVGIGTTMLLHCDLVYATPQARFQLPFVPLGLVPEAGSSLLLPLLAGYHRAAELLLLG
                     QPFSADKALAAGIVTEVVPAEELLEYAQDAAAAVAALPPESVRLTKALMKKRDAADVS
                     ARMAEDGRLFLERVGSPEAKEAMAAFFEKRKPDFSRF"
     gene            2013..>2758
                     /locus_tag="VD791_02880"
     CDS             2013..>2758
                     /locus_tag="VD791_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV06947.1"
                     /translation="MPTAAKKFKLLLPSTMAQAGWQVLAPRPDVEAVAYDTNMPAAAF
                     HALLADADGVALSLNSFGAAEIAAGPRVRVVARHGVGYDSVDVPALTRSGIPLLVTGI
                     ANSPSVAEQALHFMLALAKHGAALHAMVQSNRWGERLKEELPSDLFGKTVLVVGFGRI
                     GTRIARACLALGMTVRVYDPYVAAKTIDAAGCVPESDLDAALPHADFVTIHCPKSAET
                     TGMFGAQRLARMKASACLINTARGGIVDEDA"
BASE COUNT          412 a          980 c          923 g          443 t
ORIGIN      
        1 cgcttccgcg acgcggaacc agaggaactg ctcgtcgcgg aatttcttcc aggcggggta
       61 gatgcgcttc cagtgcgcgc tcttctccat cagttcggcg tacagctcgt aggtggcctt
      121 ctcgcaggcc tccatcaccg atcgggggaa cggccgcagc ttggtgccgc tggcgatcag
      181 cttgcgcagc gattgcggat tctccgcatc gtacttggcc ggcatccaca cgcacgcctc
      241 tccgcacgcc gcgtgcagcg ccgactgata ggccttgggc agctcgttgt acttcttctc
      301 gttgaccagc atcagcccga tcggaccggc ttcccaccac ccggggtagt agtagaactt
      361 ggcgaccttc tggaagccga gcttctcgtc gtcgtacggc cccacccatt cggcaccgtc
      421 gatggtgccc ttctccagcg acgggtagat gtcggcgccg ctgatcatct gcggcaccac
      481 gccgagcttg gcgagcacca gcccggccat gccgcccatg cggaacttga gccccttcat
      541 gtcggcgacg ctcttgatct ccttgcggaa ccagccgccc atctggcagc cggtgttgcc
      601 cgccgggatg gcgatgcagc cgtagtcctt gtacaggggc gccatcgcct gcgcgccgcc
      661 gccgaagaac caccaggcga agttctggcg cgcgttcagg ccgaacggga cggcggtgcc
      721 gaaggagaac gccggatcct tgccgaagta gtaatacgat gcggtgtgcc ccatttccac
      781 ggtgccggcc tggaccgcgt cgagcacctg caacggcggc acgatctcgc cgccggcgaa
      841 cacccggatc tggaacttgc cttcggtcac ctcggacacg cgccgcgcga ccagctccgc
      901 gccgccgaac agcgtgtcca ggctcttcgg ccagctcgcc gccagccgcc actggatgct
      961 gggctgcgca tgcacgatgg ccggcgcggc gaccgcgccg gtcgccatcc ccgcggccgc
     1021 ggtcttgagg aaagatctgc gcttcatggt ctcctccgga agtcgagttg atgtgttgtt
     1081 ttcagcagga ccggccggcg cgcgccgccg ccctgtcctc agaatcgtga gaaatccggc
     1141 ttgcgcttct cgaagaaggc cgccatcgcc tccttcgctt cgggcgaacc cacgcgctcg
     1201 agaaacagcc ggccgtcctc ggccatgcgc gccgacacgt ccgcggcgtc gcgcttcttc
     1261 atcagcgcct tggtcaggcg caccgattcg ggcggcagcg cggccaccgc cgcggccgca
     1321 tcctgcgcgt attccagcaa ctcctccgcc ggcaccacct cggtgacgat gccggccgcg
     1381 agcgccttgt ccgccgagaa aggttggccc aggagcaaca gctcggccgc gcgatggtat
     1441 ccggcgagca gcggcagcag caggctggag cctgcttccg gcaccagtcc gagcggcacg
     1501 aacggcagct ggaagcgcgc ttgcggcgtg gcgtacacca ggtcgcagtg cagcagcatg
     1561 gtggtgccga tgcccacggc ggggccgccc accgccgcga tcagcggctt ggctgccgtc
     1621 gagatcgcct gcaggaactg aaacaccggc gagcgctccg cgtgcggcgg gcgctcgagg
     1681 aagtccttca gatcgttgcc cgcggagaag caatccggct ggccgtggat caggccgacg
     1741 cgtacctgcg gatcggcctc gacggcgcgc agcgcgtcgg ccatcgcctg gtacatctcc
     1801 accgtgagcg cgttcttctt gtcggggcgc ttcagctcga tgcgcgcgac gcgctcgcgg
     1861 gtctcggtca ggacatgcgc ggacatggct ccttgctcct tattgccgga cgggctgcgg
     1921 gcggtcgccg gcacagcgca gtgtaaccgc atcgcgcgca gacggcaggc gttccaggta
     1981 cagtgtcgcc gtcaccggag gagacccgca gaatgcccac cgccgccaag aagttcaagc
     2041 tgctgctgcc gtccaccatg gcccaggccg gctggcaggt gctggcgccc cgccccgatg
     2101 tggaggcggt cgcctacgac acgaacatgc ccgcagcggc gttccatgcg ctgctggccg
     2161 acgcggacgg cgtggcgctg agcctcaact cgttcggcgc ggccgagatc gcggccgggc
     2221 cgcgcgtgcg cgtggtggcg cgtcacggcg tgggctacga ctcggtggac gtgccggcgc
     2281 tcacccgcag cggcatcccg ttgctggtga ccggcatcgc caactcgccc tcggtcgcgg
     2341 agcaggcgct gcacttcatg ctggcgctcg ccaagcacgg ggcggcgctg cacgccatgg
     2401 tgcagagcaa ccgctggggc gagcggctca aagaggagct gcccagcgat ctgttcggca
     2461 agaccgtgct ggtggtgggc ttcggccgca tcggcacgcg catcgcccgc gcctgcctgg
     2521 ccctgggcat gacggtgcgg gtctacgacc cttacgtcgc ggccaagacg atcgacgcgg
     2581 ccggctgcgt gcccgagagc gacctcgatg ccgcgctgcc gcacgccgat ttcgtcacca
     2641 tccattgccc gaagagcgcc gagaccactg gcatgttcgg tgcgcagcgg ctcgcccgca
     2701 tgaaggcgag cgcctgcctg atcaacacgg cgcgcggcgg catcgtcgac gaggatgc
//
LOCUS       DASPGE010000092         3766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1316210, whole genome shotgun sequence.
ACCESSION   DASPGE010000092 DASPGE010000000
VERSION     DASPGE010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316210"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1853)
                     /locus_tag="VD791_02885"
     CDS             complement(<1..1853)
                     /locus_tag="VD791_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXV06948.1"
                     /translation="MLIAAAMAAGLVLTAPVAHARPSVVLMSLDGAKPELVERYLRMG
                     VLDREGLGRLRTHGIFAHRNITATPSVTAVSHIAIATGSTAVHNNIPGNAFHPVAATI
                     GTTISGFGAPIGGYRIDPLGAPAAPTAQPLWVRVREAGLKVVTATWPGSDGADIRIAS
                     TLVQPAAPARITDYSVPFGAFSGLGAQGFALTAADFEDAPQPLVEQLAAAGRSSYSPV
                     RVTIDPIQTLFCAPMAGSGCGTTDAGGRTLRYDLKAAALDTSDDAAVNYDTLAVFDAN
                     AAVPPGPFTPPSTGAAYVQRGGPSGRFFFEGSGNRIGTAFFVSHLAPDLSAVRLVRYA
                     ATFIPRNAAVIDVVDEVNGRVGFWAPQPDFRILERLSPGFTAFPELELEALYRDQVAT
                     FTDYQTALALHAMRRNADADLVMIYLEQPDGSGHQFTLTDPRQPTDSADPASIGTPRN
                     PPGASGQDAAKVKRYAEHLAFAYQRADRAVQRILQEVGVDQHGAPLRDVFVVSDHGMA
                     PFHTAVRLANLLANAGIDTGALAVRTTGPAANIYVNLAGRESGGSVAPGDYETLVERI
                     AAALRAAADPNGFYNPSGGALFSHVWTRPGGCGKPGFCTDDRVGQDSGDVVA"
     gene            complement(1903..>3766)
                     /locus_tag="VD791_02890"
     CDS             complement(1903..>3766)
                     /locus_tag="VD791_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HXV06949.1"
                     /translation="GGRMSEESAATTPVTRWLRWTVATVAVTMSLYHMYVAAFGPPEA
                     MIFRGTHLLFALTLVFLLFPLRPGAHWRALDALLLAGGWACVLYIFFNYEYYTNRIIY
                     IDELNFWDRFYAVLAVLVVLEGTRRVIGWALPLTALAFLAYVVFFTNVAPPVLLEQLY
                     LSTEGIFGSTLGVSASYVMLFVLFGAFMERSGTGQLFMDFAMSITGHSAGGPGKVAVV
                     SSSLFGTVSGSAVANVMVDGPITIPLMKRTGFRPPFAAAVEAVASTGGQLMPPIMGAA
                     AFVMAEFLAVPYVQVALWAAIPALLYYVAVFFAVHFEAKRCGLAGVPREQLPRFARVI
                     AARGHLFAPILVILTGLFLGYSAPLCALAGALSCLPIALVRATTRAGITWRTVLEALE
                     DGARNTLAVAMACACAGIVIGCVTITGLGITFTQLVIALAQNSLVLALVLTALAGIIL
                     GMGMPTTPAYIVMVALLVPAVIKLGAVTPAAHMFAFYFAILSAITPPVALAVFAAAGL
                     AKANMWTAGWAAMRAGAPAYIVPFMFVYEPALLLIGDWTVSLHATITATVGVILLAGG
                     LFGYLARPATPWQRALLIAAALLLIKPGWATDLVGLGLAAVVVAAQLARPRETA"
BASE COUNT          655 a         1304 c         1319 g          488 t
ORIGIN      
        1 gccaccacat cgccggagtc ctggccgacg cggtcgtcgg tgcagaagcc aggcttgccg
       61 cagccgcctg gccgcgtcca cacatgcgag aacagcgcac cgcccgaagg gttgtagaag
      121 ccgttgggat ccgccgcggc acgaagagcg gctgcgatgc gctcgaccaa ggtctcataa
      181 tcgcccggcg ccacgctgcc gcctgattcg cggcccgcga ggttgacgta gatattcgcc
      241 gccggcccgg tggtgcgcac cgcgagcgcg ccggtgtcga tgccggcgtt cgcgagcaga
      301 ttagccagcc gcacggcagt gtgaaacggc gccatgccgt gatcggaaac cacgaacacg
      361 tcgcgcaggg gcgcgccgtg ctggtccact cccacttctt gcaggatgcg ttgcaccgcc
      421 cgatcggcgc gctgataggc gaacgccagg tgctccgcgt agcgcttcac cttcgcggcg
      481 tcctggccgc tggctccggg cggattccgc ggcgtgccga tgctggccgg gtccgccgag
      541 tcggtgggct gccgcggatc ggtcaaggtg aactggtgtc cggatccatc gggctgctcc
      601 agatagatca tcaccaggtc ggcgtcggcg ttgcgccgca tcgcgtgcag cgcgagcgcg
      661 gtctggtagt cggtgaaagt cgcgacctgg tcgcgataca gcgcctccag ctccagctcc
      721 ggaaacgcgg tgaagcccgg gctcaggcgc tcgagaatgc ggaagtcggg ctggggcgcc
      781 cagaagccga cgcgaccgtt gacctcatcg accacgtcga tcaccgctgc gttgcgcgga
      841 atgaaggtcg ccgcgtagcg cacgaggcgc accgcggaaa ggtcgggcgc cagatggctg
      901 acgaaaaatg ccgtgccgat ccgattgccc gagccttcga agaagaatct gccggacggc
      961 ccgccgcgtt gtacgtacgc agccccggtg gaaggcggcg tgaacggccc gggcggcacg
     1021 gcggcattgg cgtcgaagac ggcgagggtg tcgtagttga ccgccgcatc gtcgctcgtg
     1081 tccagcgcag ccgccttgag gtcgtagcgc agcgtccgcc cgccggcatc cgtggtaccg
     1141 cagccgctgc cggccatcgg tgcgcagaac aaagtctgga tcgggtcgat cgtcacccgc
     1201 acgggactgt acgaactgcg ccccgccgcc gcgagctgtt cgacgagcgg ctgcggggcg
     1261 tcctcgaaat ccgccgcggt cagcgcaaaa ccctgtgcac ccaagccact gaaagcgccg
     1321 aacggcacgc tgtagtcggt gatgcgcgca ggcgcggccg gctgcaccag ggtgctggcg
     1381 atgcggatgt cggcgccgtc gctgcccggc caggtggcgg tcaccacttt gagccccgcc
     1441 tcgcgcacgc gcacccacag cggctgcgcg gttggcgccg ccggcgcgcc gagcgggtcg
     1501 atccggtaac cgccgatcgg cgcgccgaag ccgctgatgg tcgtgccgat cgtcgccgcc
     1561 accggatgaa aggcgtttcc cggaatattg ttgtggaccg ccgtcgaacc ggtggcgatg
     1621 gcgatgtgcg aaacggccgt gaccgagggc gtcgcggtga tgttgcggtg cgcgaagatg
     1681 ccgtgcgtgc gcaagcgtcc cagtccttct cgatcgagca cgcccatgcg cagatagcgc
     1741 tcgaccagtt ccggcttggc gccatcgagg gacatcagca ccacgctcgg tcgggcatgt
     1801 gccaccggtg cggtcagcac caggccagcg gccatcgccg ccgcgatcag catgtccgtc
     1861 ggcgcagcgc gcgtggatcg tcgcactttc gggacgaccc gctcaggcgg tctcgcgcgg
     1921 ccgagcgagc tgcgccgcca cgacgacggc ggcgaggccg agcccgacga gatcggtggc
     1981 ccagcccggc ttgatcagga gcaacgcggc cgcgatcagc agcgcgcgct gccacggcgt
     2041 ggccgggcgc gccaagtagc cgaacaaccc gcctgcgagc aggatcacgc cgacggtcgc
     2101 ggtgatcgtc gcgtgcagac tcaccgtcca atcgccgatg agcagcaggg ccggctcgta
     2161 gacgaacatg aacggcacga tgtaggccgg cgccccggcg cgcatcgccg cccagcccgc
     2221 cgtccacatg ttcgccttgg cgaggcccgc cgcggcgaac accgccagcg ccaccggcgg
     2281 ggtgatcgcc gacaggatgg cgaagtagaa cgcgaacatg tgcgccgccg gcgtcaccgc
     2341 gccgagcttg atcaccgccg gcaccagcag cgcgaccatc acgatgtagg ccggcgtggt
     2401 cggcatgccc atccccagga tgatgccggc gagcgcggtc agcaccaacg cgagcaccag
     2461 cgagttctgc gccagcgcga tgaccagctg cgtgaacgtg atgcccagcc cggtgatcgt
     2521 cacgcagccg atgacgatgc cggcgcaggc gcaggccatc gccaccgcca gggtgttgcg
     2581 cgcgccgtcc tcgagcgcct cgagcaccgt gcgccaggtg atgccggcgc gcgtggttgc
     2641 gcgcaccaga gcaatcggca gacacgaaag cgcgcccgcc agcgcgcaca gcggcgccga
     2701 gtagccgagg aaaagaccgg tcaggatcac caggatcggc gcgaacaggt ggccgcgcgc
     2761 cgcgatcacg cgggcgaagc gcggcagctg ctccctcggc accccggcga gcccgcagcg
     2821 cttcgcctcg aagtgcaccg cgaagaacac cgccacgtaa tagagcagcg cgggaatcgc
     2881 cgcccacagc gcgacctgca cgtacggcac cgccaggaac tcggccatca cgaaggcggc
     2941 cgcgcccatg atcggcggca tcaactgtcc gccggtcgag gccaccgcct cgaccgcggc
     3001 cgcgaacggg gggcggaacc cggtgcgctt catcagcggg atggtgatcg ggccgtccac
     3061 catgacgttg gccacggcgc tgcccgacac cgtgccgaac agcgacgagc tgaccacggc
     3121 gaccttgccc ggaccgccgg ccgagtgccc ggtgatcgac atcgcgaagt ccatgaacag
     3181 ctgcccggtg ccgctgcgct ccatgaacgc gccgaacagc acgaacagca tcacgtagga
     3241 ggccgacacg ccgagcgtgg agccgaagat cccctcggtc gagaggtaga gctgctccag
     3301 aagcaccggt ggggccacgt tggtgaagaa caccacgtag gcgaggaatg cgagcgcggt
     3361 gagcggcagc gcccagccga tgacgcggcg cgtgccctcc aggaccacga gcaccgccag
     3421 aacggcgtag aacctgtccc agaagttgag ctcgtcgatg tagatgatgc ggttggtgta
     3481 atactcgtag ttgaaaaaga tgtacaggac gcaggcccag ccgccggcga gcagcagcgc
     3541 gtcgagcgcg cgccagtgcg cgccggggcg cagcgggaac agcaggaaca cgagcgtcag
     3601 ggcgaacagc aggtgggtgc cgcggaagat catcgcctcc ggcgggccga atgcggcgac
     3661 gtacatgtgg tacagcgaca tcgtcacggc cacagtcgcc accgtccatc gcagccaccg
     3721 cgtcaccggt gtggtggcgg cactctcctc cgacatgcgc cctccc
//
LOCUS       DASPGE010000093        10836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_849053, whole genome shotgun sequence.
ACCESSION   DASPGE010000093 DASPGE010000000
VERSION     DASPGE010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10836
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849053"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VD791_02895"
     CDS             <1..207
                     /locus_tag="VD791_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HXV06950.1"
                     /translation="NKHRTVIEDGAFIGSDTALVAPVTVGRDATIGAGSVITEDAPAG
                     ELSVARGRQVTIKGWKRPKKARDR"
     gene            267..2096
                     /gene="glmS"
                     /locus_tag="VD791_02900"
     CDS             267..2096
                     /gene="glmS"
                     /locus_tag="VD791_02900"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HXV06951.1"
                     /translation="MCGIVGAIAQRDVVPVLLEGLRKLEYRGYDSAGLAVVNGELRRL
                     RSVGRVASLAQLAAQSALAGHIGIAHTRWATHGAPTERNAHPHVSDDGLAVVHNGIIE
                     NHEAMRARLAGLGYRFVSDTDTEVLAHLVHYYFKQTGDLFGATHRAISELTGAYAIAV
                     VSQKDPDRFVAARKGAPLLLGLGEGENFCASDASALLQVTRRIVYLEEGDVVELTCHG
                     YRIRDSAGGDAIRPVHESKLSADAVELGPYRHYMQKEIFEQPGAVAATLEMVTGASAI
                     APELFGVQARSILDELEGVSVLACGTSYHAGMVARYWFESIAGLPCSVEVASEYRYRT
                     SVPNPNALILTISQSGETADTIAALQHAKQLGQERTLAICNVAESALVRNTALCFLTR
                     AGPEIGVASTKAFTTQLAALFLLTLVLAKLRGRLPADGERAYLKALRHLPAALVSVLE
                     LEPAIARWAEHFATKQHALFLGRGVHYPIALEGALKLKEISYIHAEAYPAGELKHGPL
                     ALVDRDMPVVAIAPNDALLEKLKSNLQEVRARGGVLYVFADADSQVSESEGVHILRLT
                     ENRGHLSPILHVVPLQLLAYHAALVRGTDVDKPRNLAKSVTVE"
     gene            2449..2904
                     /locus_tag="VD791_02905"
     CDS             2449..2904
                     /locus_tag="VD791_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV06952.1"
                     /translation="MRRVKTLLLALALAAAPAWANLGEEEEESSNPDWIEGKKAVEAQ
                     DWKRAVPLLSKAAEAEPKNADIRNWLGFANRKLGNMDAAFAAYNEALKLNPRHKHAHE
                     YIGEAYLMANDVAKAEQHLAELQKLCTPIPCEEYKDLKRAVDEYKKSKK"
     gene            2907..3566
                     /locus_tag="VD791_02910"
     CDS             2907..3566
                     /locus_tag="VD791_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXV06953.1"
                     /translation="MPNPAIRAVLWDFGGVLTESPFEAFQRYERSHGLPAGFIRRLNA
                     VNSDRNAWARLERGEITLTEFDLAFAEEARSAGQTVPGSEVVALLYGAVRPPMVEALR
                     RCGAHYLTACLTNNINTGVGHGLPASDERAAEVGRILQLFDVVIESSKVGVRKPEPGF
                     YRMALDALKIEAPQAVYLDDLGVNLKPARAMGMITIKVESTEQALGELEQRLELTLRD
                     Q"
     gene            complement(3634..4401)
                     /locus_tag="VD791_02915"
     CDS             complement(3634..4401)
                     /locus_tag="VD791_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV06954.1"
                     /translation="MSAQRVVITGASSGIGAALAREYARRGATLGLIARRRDPIERLA
                     AELPGTHATYAVDVRDAHALRAAAEQFCARFGGADVVIGNAGVSSGTLTDRAEDEPVF
                     REILDINVLGLFNTFQPFLASMRTARRGTLAGIASVAGFRGLPGAGAYSASKAAAIAY
                     LESLRVELRGSGVHVVTVSPGYVATPMTERNPYPMPFLLNADEAARRIVRAIERRKPH
                     SVIPWQMGVVGGVLRALPRPLYDRLFARAPRKPRRSG"
     gene            complement(4398..5087)
                     /locus_tag="VD791_02920"
     CDS             complement(4398..5087)
                     /locus_tag="VD791_02920"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HXV06955.1"
                     /translation="MHASRRRLLRALTIGGMALGARHAAAVETIGAAVEVGAAVDLLE
                     EVDYRLIARQPLADPARIEVVEFFYYGCHWCSEFEPHLNDWLGRKPADVVFRYQPAIR
                     NTRWLVLTKAFYAFRALGVQPQLHARIYRAYHREEINLEDEAVLTAWALKQGVALKPL
                     ERLLGSDEVMAEVQAARAATDAYEIETTPSVVVDGRYLTSSGMIGGVGELMRVVEDLI
                     VLARQERPARG"
     gene            complement(5095..5781)
                     /locus_tag="VD791_02925"
     CDS             complement(5095..5781)
                     /locus_tag="VD791_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HXV06956.1"
                     /translation="MRFPRLFGWSSLLFAVATLAAGALAQPPIADKEYKLINPPQRTE
                     TGKKIEVIEFFSYACPHCADFEPFLQDWLKRKPKDVEYRMVPMVFRESWKPLAKLYFT
                     LEQMALVDKYHMKVYDALHKQNQQLFTDQAVMDWVLKQGIDKAKFEQTYHSFGIDTKV
                     QRAAAMGRAYGVQFTPSLAVNGKYWTGPSMVMGPNGGLDIPRFFEVVDRLVAMERGKP
                     AAAGGTKEKS"
     gene            complement(5821..6444)
                     /locus_tag="VD791_02930"
     CDS             complement(5821..6444)
                     /locus_tag="VD791_02930"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXV06957.1"
                     /translation="MARAQRGTAVRSSGAIGHPMLTGVFIGLLIGLSMALAVALYLNK
                     YNPFVARSRPPDKPEPRVEAAKVAPPAGMPTGRRPQAQPPRPQDARQADAKRFDFYKI
                     LPGTEEAVSEKDLKRNRPPGAAREVYYLQAGAFQKSADADNLKARLALAGLEAQIQTA
                     TLPDQTVWHRVRLGPFRHPDAMMQTRSVLRENQIEPSIIKVAERDTR"
     gene            complement(6453..8198)
                     /gene="argS"
                     /locus_tag="VD791_02935"
     CDS             complement(6453..8198)
                     /gene="argS"
                     /locus_tag="VD791_02935"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXV06958.1"
                     /translation="MASPAIQDLKAHLASLFSRALQEVAPDASGFSVTVERPKAAEHG
                     DFACNLALQLAKPLKRNPREIAQALVSALPASPVIDRTEVAGAGFINLFLRPAAKRAV
                     VRQVLERGDAYGRIGVGQGKKVQVEFVSANPTGPLHVGHGRGAAYGASLSNVLAAAGF
                     DVQREYYVNDAGRQMDILALSTWLRYLELQAVVVPFPANAYQGDYVRDMAAAIAAAHG
                     KRYVRAAEQVMHNVPAVEDDAEAHLDALIANAKRILGGDYAYVHDYALNEQLGDCRND
                     LLEFGVTFDHWFSERSLFEAGHVLQALEHLQQAGQLYRQDGAMWFRSSVFGDEKDRVV
                     QRKNGEYTYFASDIAYHVNKFERGFDLAIDVWGADHHGHVPRVKGALQALGLDPARLT
                     VALVQFAVLYRGGKKAAMSTRAGDYVTLRQLRAEVGNDAARFFYVLRKSDQHLDFDLD
                     LAKSQSTDNPVYYVQYAHARICSVLDKWGGHVPDLRTAELAPLEGEQELKLLRQLMEY
                     PQTIEDAARELSPHLVAFYLKDLAGAFQSYYQSVPFLVEDAAVRDARLALVAAIAQVL
                     RNGLQLLGVSAPEKM"
     gene            8427..9281
                     /locus_tag="VD791_02940"
     CDS             8427..9281
                     /locus_tag="VD791_02940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXV06959.1"
                     /translation="MAGCVGLTLVTATVRFSLEEHLRVSLTLLPPIMLVAWYVGMGWG
                     LSAACVVVLSWLAADLLAVTPPAPSWVAYVNAGVRAGVFGLIAALVAALRAAYRQQRL
                     LATQDALTGLGNRHRFMAVAEIERQRATRFGHALALAYLDLDDFKAVNDALGHAQGDE
                     VLRVVGRFLHKRLRSIDHAARLGGDEFVVLLPASGVQAVARGLADVHQGLMDALRHAG
                     FAVGVSAGIAIFPHAPPSVAAMLQAADRLMYQAKLQGKSQARQAVIGGGEAQGASAGS
                     AEAGAHRR"
     gene            complement(9339..>10836)
                     /locus_tag="VD791_02945"
     CDS             complement(9339..>10836)
                     /locus_tag="VD791_02945"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004067.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HXV06960.1"
                     /translation="TEVYLQSVAALLQRGRQALVLVPEINLTPQLIERFRTRFAGVPL
                     AAMHSNLAEGERLRAWRAAERGEVGVVIGTRLAVFTPLPQLGVIVVDEEHDASFKQQE
                     GLRYSARDLALLRAKLRGIPVVLGSATPSLESYANAIAGRYALLELPDRPGAASPLIR
                     CVDTRGQGLSHGLSQPLLDALRGRVARGEQSLVFVNRRGYAPALVCPACAWVAPCTRC
                     SAKLVLHLGDGKLRCHYCGHEEPITAVCPGCGNQDLMPAGHGTQRLEAALLEAVPGAR
                     VLRVDRDTTRRRHSFEAMQGQIRGQQVDILVGTQMLAKGHDFPRLTLVGVVNADSALY
                     SGDFRASERLYAQLTQVAGRAGRSDLPGEVLIQTDFPEHPLYDAVCRQDYAGFARAAL
                     DERRQAGFPPHAYQALLRAEAGRREVVDDYLTRAAAAGVALGLAVEVYDPVPPPIARI
                     GGRERGQLLVQSRSRSELQRFLHAWYPQLAAQASRAVRWSLDVDPLEL"
BASE COUNT         1719 a         3742 c         3733 g         1642 t
ORIGIN      
        1 aacaagcatc gcaccgtcat cgaagacggt gctttcatcg ggtcggacac cgcgctggtc
       61 gccccggtca cggtggggcg tgacgccacc atcggcgccg ggagcgtcat caccgaggac
      121 gcacccgcgg gcgagctgtc ggtggcccgg gggcggcagg tgacgatcaa aggctggaag
      181 cggcccaaga aggcccgcga tcggtgatca tcggcgatcg atgatgagaa cctcaatcac
      241 gaatcacgaa tcacgaatca cgaatcatgt gcggcatcgt cggcgcaatc gcgcaacgtg
      301 acgtagtccc ggtcctgctg gaggggctgc gcaagctgga ataccgcggc tacgactccg
      361 ccgggctggc ggtggtcaac ggcgagctgc ggcggctgcg cagcgtcggc cgggtcgcgt
      421 ccctcgctca gctcgcggca cagtcggcgc tggccggcca catcggcatc gcgcacacgc
      481 gctgggccac gcatggcgcg cccaccgagc gcaatgccca cccgcacgtc agcgacgacg
      541 gcctggcggt ggtgcacaac ggcatcatcg agaatcacga ggcgatgcgc gcgcgcctgg
      601 cgggactcgg ctaccgcttc gtgtccgaca ccgacaccga agtcctggcg cacctggtgc
      661 actattactt caagcagacc ggggacctgt tcggtgcgac gcaccgcgcg atcagtgagc
      721 tcaccggcgc ttacgccatc gccgtggtct cgcagaagga ccccgatcgg ttcgtggcgg
      781 cgcgcaaggg cgcgccgctg ctgctgggac tgggagaggg tgagaacttc tgcgcctcgg
      841 acgcttccgc cctgctgcag gtcacgcgcc gcatcgttta tctggaggag ggcgacgtcg
      901 tggagctgac ctgccacggc taccgcattc gggactccgc cggcggcgat gcgatacgcc
      961 ccgtgcacga gagcaagctc tcggcggacg ccgtggagct gggcccctac cgccactaca
     1021 tgcagaagga gatcttcgag cagcctgggg cggttgcggc caccttggag atggtcacgg
     1081 gcgcgagcgc catcgccccg gagctgttcg gagtgcaagc gcgcagcatc ctggacgagc
     1141 tcgagggggt gagcgtgctg gcgtgcggca ccagctatca cgccgggatg gtggcgcgct
     1201 actggttcga gtccatcgcc gggctgccct gcagcgtgga ggtcgccagc gagtaccgct
     1261 accgcaccag cgtgccgaat cccaatgcgc tgatcctcac catctcccag tccggggaga
     1321 cggccgacac gatcgcggcg ctccagcacg ccaagcagct cgggcaggag cgcacgcttg
     1381 ccatctgcaa cgtcgcggaa agcgcgctgg tgcgcaacac ggcgctgtgc ttcctcacgc
     1441 gcgcgggacc ggagatcggc gtggcctcca ccaaggcgtt caccacgcaa ctggccgccc
     1501 tgttcctgct gaccctggtg ctggccaagc tgcgcgggcg tctgccggcg gacggcgagc
     1561 gggcctacct caaggccttg cgccacctgc ccgcggcgct ggtcagtgtg ctggagctgg
     1621 agccagccat cgcccgctgg gccgagcact tcgccaccaa gcagcacgcg ctgttcctgg
     1681 ggcgcggcgt gcactacccg atcgccctgg agggcgcgct gaagctgaag gaaatctcct
     1741 acatccacgc cgaggcctat ccggccggcg agctgaagca cggcccgctc gccctggtgg
     1801 accgcgacat gccggtggtg gcgatcgcgc ccaacgacgc gctgctggag aagctcaagt
     1861 ccaatctgca ggaggtgcgc gcgcgcggcg gcgtgctgta cgtgttcgcc gacgccgaca
     1921 gccaggtgtc ggagtcggaa ggcgtgcaca tcctgcgcct caccgagaac cggggacact
     1981 tgagccccat cctgcacgtg gtgccgctgc agctgctcgc ctaccacgcc gcgctggtgc
     2041 gcgggaccga tgttgacaag cctcgaaatc tcgccaaatc ggttacggtt gagtaagcgg
     2101 ccaacgcagc gcccaacgca cggttgccag gtggcttgcg gcgtaggcta acgtgcggct
     2161 tgccgcacgt taaggccgct tcagcggccg gccgcaggca caagcctcgg aacctagcca
     2221 aatcggtaac ggttgagtga gcggccaacg tcgcgcccaa cgcacggttg ccagatggct
     2281 tgcggcggag gctaacgtgc gcttgcgcac gttaagggcg cttcagcgcc cggccggagt
     2341 cataaacccc gcaacttggc gaaatcagta acggtggagt gacgaccggg gcattgacag
     2401 ccggcctgcg ggcgggcacc ttttccgctc ggcagacgga ggagcaccat gagacgcgtc
     2461 aagaccctgc tgctcgcgtt ggcactggcc gcggcgccgg cctgggccaa cctgggggaa
     2521 gaggaagagg agagcagcaa ccccgactgg atcgagggca agaaggcggt cgaagcgcag
     2581 gactggaagc gcgccgtgcc gttgctgtcc aaggccgccg aagcggagcc caagaacgcg
     2641 gacatccgca actggctcgg cttcgccaac cgcaagctcg gcaacatgga tgcggccttc
     2701 gccgcgtaca acgaggcgct caagctcaat ccgcgccaca agcatgcgca cgagtacatc
     2761 ggcgaggcct acctgatggc gaacgacgtc gccaaggcgg agcagcacct cgccgagctg
     2821 cagaagctgt gcacgccgat cccttgcgag gagtacaagg acctgaagcg cgcggtggac
     2881 gaatacaaga agagcaagaa gtagcgatgc cgaacccggc gatccgcgcc gtcctgtggg
     2941 atttcggcgg cgtgctcacg gagagcccgt tcgaggcgtt ccagcgctac gagcgcagcc
     3001 acggattgcc ggccggcttc atccggcggc tcaacgccgt caactccgac cgcaacgcct
     3061 gggcgcgcct ggagcgcggc gagatcaccc tgacggaatt cgaccttgcc ttcgccgagg
     3121 aggcgcgcag cgccggccag acggtgccgg gttcggaagt ggtggcgctg ctgtacggag
     3181 cggtgcggcc gccgatggtg gaagcgctgc gccgctgcgg cgcgcactac ctgaccgcgt
     3241 gcttgacgaa caacatcaac accggtgtcg ggcacggcct gccggcaagc gacgagcgcg
     3301 ccgccgaggt ggggcgcatc ctgcaactgt tcgatgtggt gatcgaatcc agcaaggtcg
     3361 gcgtgcgcaa gccggagccg ggcttctacc ggatggccct ggatgcgctg aagatcgaag
     3421 cgccgcaggc cgtgtacctc gacgacctgg gcgtcaatct gaagccggcg cgggcgatgg
     3481 ggatgatcac catcaaggtc gaaagcaccg agcaggcgct cggcgagctg gagcagaggc
     3541 tcgagttgac cctgcgcgat cagtgaccgc gcgggagcag ccgctgcccg caggggaaag
     3601 cgaacggcct cttcgcccag tcgttgccgg gcgtcaaccg gaccgtcgcg gcttgcgcgg
     3661 cgcgcgtgcg aacagccggt cgtagagcgg ccgcggcagc gcccgcagca cgccgcccac
     3721 cacgcccatc tgccagggaa tgacgctgtg cggcttgcgc cgctcgatcg cgcgcacgat
     3781 gcggcgcgcg gcttcgtcgg cgttcaggag gaaaggcatc ggataggggt tgcgctcggt
     3841 catcggcgtg gcgacatagc ccggggacac ggtcaccacg tgcacgccgc tgccgcgcag
     3901 ctcgacgcgc aggctctcga ggtaggcgat ggcggccgct ttcgacgccg agtaggcgcc
     3961 cgcgcccggc aggccgcgga agccggcgac gctggcgatg cccgcgagcg tgccccggcg
     4021 cgcggtgcgc atggacgcca gaaacggctg gaaggtgttg aacagcccca gcacgttgat
     4081 gtcgaggatc tcgcggaaca cgggctcgtc ttcggcgcgg tcggtgagcg ttccgctcga
     4141 caccccggcg ttgccgatca ccacgtctgc gccgccaaag cgtgcgcaga actgctccgc
     4201 cgccgcgcgc agtgcatgcg catcgcgcac gtccacggca taagtcgcat gcgtgccggg
     4261 cagctcggcc gccagccgct cgatcggatc gcgccgccgc gcgatcaacc cgagcgttgc
     4321 accgcgccgc gcatattcgc gcgccagcgc cgcgccgatg ccgctcgagg cgccggtgat
     4381 caccacccgc tgcgcgctca tccgcgcgct ggccgctcct gcctggccag gacgatgaga
     4441 tcctccacca cgcgcatcag ctcgccgacg ccgccgatca tgccgctgct ggtgaggtag
     4501 cgtccgtcca ccaccaccga gggcgtggtc tcgatctcgt aggcgtcggt cgccgcgcgc
     4561 gccgcctgca cctcggccat cacttcgtcg gagccgagca gccgctccag aggcttgagc
     4621 gccaccccct gcttcagcgc ccaggcggtg agcaccgcct cgtcctcgag gttgatctcc
     4681 tcgcgatggt aggcgcggta gatgcgcgcg tgcagctgcg gctgcacgcc cagcgctcgg
     4741 aaggcgtaga acgccttggt cagcaccagc cagcgcgtgt tgcggatcgc gggctggtaa
     4801 cggaagacga cgtccgccgg cttgcgcccg agccagtcgt tgagatgcgg ttcgaactcg
     4861 ctgcaccagt ggcagccgta gtagaagaac tcgaccacct cgatgcgcgc gggatcggcc
     4921 agcggttgtc gtgcgatcag gcggtagtcg acctcttcca gcagatcgac ggcggcgccc
     4981 acttcaacgg cagcgccgat cgtttcgaca gcggcagcgt gccgcgcccc gagcgccatc
     5041 ccgccgatcg tcagtgcccg cagcaggcgc cggcgcgagg catgcatgcg cgcctcagct
     5101 cttctccttg gtgccgccgg cggccgccgg cttgccgcgc tccatggcga ccagccggtc
     5161 caccacctca aagaagcgcg ggatgtccag cccgccgttg gggcccatca ccatcgacgg
     5221 gccggtccag tacttgccgt tgaccgccag cgacggggtg aactgcacgc cgtaggcgcg
     5281 gcccatggcg gcggcccgct gcaccttggt gtcgatgccg aaggagtggt aggtctgctc
     5341 gaatttggct ttgtcgatgc cctgcttgag cacccagtcc atcaccgcct ggtcggtgaa
     5401 cagctgctgg ttctgcttgt gcagggcgtc gtagaccttc atgtggtact tgtccaccag
     5461 cgccatctgc tccagagtga agtacagctt ggcgagcggc ttccagctct cgcggaacac
     5521 catcggcacc atgcgatact ccacgtcctt cggcttgcgc ttgagccaat cctgcaggaa
     5581 cggctcgaag tcggcgcaat gcgggcaggc gtaggagaag aactcgatca cctcgatctt
     5641 cttgccggtc tccgtcctct gcggcggatt gatcagcttg tattccttgt ccgcaatcgg
     5701 cggctgcgca agcgcgccgg ccgccagcgt cgcgacggcg aacagcaggg aggaccagcc
     5761 aaacagtctg gggaagcgca tgggtgctcc tgtgcggtga ggattcgttt gcccgactcg
     5821 ttacctggtg tcgcgctcgg caaccttgat gatgctcggc tcgatctggt tctcgcgcag
     5881 cacgctgcgc gtctgcatca tggcgtccgg gtgccggaac ggccccaggc gcacacggtg
     5941 ccacaccgtc tggtccggaa gcgtggcggt ctggatctgc gcctccagcc cggccagcgc
     6001 caggcgcgct ttcaaattgt ccgcatccgc ggacttctgg aacgctccgg cctgaaggta
     6061 gtacacctcg cgcgccgcgc ccggcggccg gttgcgcttg aggtcctttt cgctgaccgc
     6121 ctcctccgtt cccggcagga tcttgtagaa gtcgaagcgt ttggcgtcgg cctggcgcgc
     6181 gtcctgcggg cggggcggct gcgcctgcgg gcgccgcccc gtcggcatgc cggcgggcgg
     6241 cgcgaccttc gccgcctcca cgcgcggctc gggcttgtcc ggcggcctgg aacgcgccac
     6301 gaacgggttg tacttgttca gatacagcgc gaccgccagc gccatgctaa gcccgatcag
     6361 cagcccgatg aacactccgg tgagcatcgg atgaccgatg gcgccgctcg atctgaccgc
     6421 agtgccgcgc tgtgctcgtg ccatggatca ggtcacatct tctccggtgc gctcacgccc
     6481 agcagctgca ggccgttgcg caggacctgg gcgatcgcgg cgaccagcgc caggcgcgcg
     6541 tcgcgtacgg cggcatcctc caccaggaac ggcaccgact ggtagtagct ctggaacgcg
     6601 ccggcgaggt ccttcaggta gaacgccacc aggtgcggcg acagctcgcg cgccgcatcc
     6661 tcgatggtct gcgggtattc catcagctgg cgcagcagct tgagctcctg ctcgccttcc
     6721 agcggcgcca gctccgcggt gcgcagatcc ggcacgtgac cgccccactt gtcgagcacg
     6781 ctgcagatgc gcgcgtgcgc atactgcacg tagtacaccg ggttgtcggt gctctgcgat
     6841 ttcgccagat ccaggtcgaa gtccaggtgc tggtcgctct tgcgcaacac atagaagaag
     6901 cgcgcggcgt cgttgcccac ctcggcgcgc agctggcgca gcgtgacgta atcaccggca
     6961 cgcgtcgaca tggcggcctt cttgccgccg cgatacagca cggcgaactg caccagcgcc
     7021 acggtcagcc gggccggatc cagacccagg gcctgcagcg cgcctttcac ccgcgggacg
     7081 tggccgtgat gatcggcgcc ccacacgtcg atcgccaggt cgaagccgcg ctcgaacttg
     7141 ttcacgtgat aggcgatgtc ggaagcgaag taggtgtact cgccgttctt ccgttgcacc
     7201 acgcggtcct tctcgtcgcc gaagacgctc gagcgaaacc acatggcgcc gtcctgccgg
     7261 tacagctgcc cggcttgctg caggtgctcg agcgcctgca gcacgtgccc cgcctcgaac
     7321 agcgagcgct ccgagaacca gtgatcgaag gtgacgccga actcgagcag atcgttgcgg
     7381 caatcgccga gctgctcgtt gagcgcgtag tcgtgcacgt aggcatagtc gccgcccaga
     7441 atcctcttcg cgttggcgat gagcgcatcc aggtgcgcct cggcgtcgtc ctcgaccgcc
     7501 ggcacgttgt gcatcacctg ctcggcggcg cgtacgtagc gcttgccgtg cgccgcggcg
     7561 atcgcggccg ccatgtcgcg cacgtagtcg ccctggtagg cgttggcggg aaacggcacc
     7621 accaccgcct gcagctccag atagcgcagc caggtggaca gcgccaggat gtccatctgc
     7681 cggccagcgt cgttcacata gtattcgcgc tgcacgtcga agcccgcggc cgccagcacg
     7741 ttggacaggc tcgcgccgta ggccgcgccg cgcccgtgcc ccacgtgcag tggccccgtg
     7801 gggttggccg atacgaattc gacttgcacc ttcttgcctt gtccgacacc gatcctgccg
     7861 taagcgtcgc cccgctcgag cacctgtcgc accaccgcgc gcttcgcggc cggcctcagg
     7921 aacaggttga tgaaaccggc gccggcgact tccgtacgat cgataacggg cgaggccggc
     7981 agggcgctga caagcgcctg ggcgatctcg cgcgggttgc gcttcaacgg cttggcgagc
     8041 tgcagcgcca ggttgcaggc gaaatcgccg tgctccgccg ccttgggccg ctccaccgtc
     8101 accgagaagc cggaggcgtc cggggcgacc tcctgcagcg cgcgcgagaa cagcgaggcg
     8161 aggtgagcct tgaggtcttg gatagccggg gacgccatgg caggccggcg gaaaaggtga
     8221 aattatagtc cggatctgcg cagggccctg cctgtgtgag acaagaagcg tcgacagcgc
     8281 cggaactttc cggcaccgat ggcgacaaac gtgttcgagc gtccagtgct cgctgcaacc
     8341 cggaccgctg catgctgcac cggtttcgat ccctcctggt agaccttctc gccgccgagg
     8401 cgacccttgc cgtaagaatc gccctcgtgg ccggctgtgt cggactgacg ctggtcaccg
     8461 ccactgtacg gttctcgctc gaggagcatc tgcgcgtctc gctcacgctg ctgcccccga
     8521 tcatgctggt cgcctggtat gtaggcatgg gctgggggct gagcgcagca tgcgtggtgg
     8581 tgctgtcctg gctggccgca gacctgctcg ccgtaacgcc gcccgcgccc tcctgggtgg
     8641 cctacgtcaa tgcgggcgtg cgggcgggcg tgttcggctt gatcgcagcg ctggtggccg
     8701 cgctgcgcgc ggcataccgc cagcaacgcc tgctcgcgac ccaggacgcg ctcacgggtc
     8761 tgggcaaccg ccatcggttc atggccgtgg cggagatcga gcgccagcgc gcaacgcgat
     8821 tcggacatgc gctcgcgctc gcctatctcg acctggacga cttcaaagcg gtcaacgacg
     8881 cgctcgggca cgcgcaggga gacgaggtgc tgcgggtggt gggccggttc ctgcacaagc
     8941 gcctgcgcag catcgatcat gcggcgcggc tgggcggcga cgaattcgtc gtgctgcttc
     9001 ccgcaagcgg cgtgcaggcg gttgcgcgcg ggctcgcgga tgtgcaccaa ggtctgatgg
     9061 acgccctgcg ccacgccggc ttcgcagtcg gcgtaagcgc cgggatcgcg atcttcccgc
     9121 acgcgccgcc ttcggtcgca gcgatgctgc aggcggccga tcggctcatg taccaggcca
     9181 agctgcaggg caagagccag gctcggcagg ccgtcatcgg aggaggcgaa gcgcagggtg
     9241 cctcggcggg ttcggccgag gccggtgcgc accggcggta acgcgcaacc gcgcttgcag
     9301 ccgcagagcg accgcttccg cgatctgcac ccgccgcgtc acagctccag cgggtccacg
     9361 tccagcgacc agcgcacggc gcgcgaggcc tgcgcagcga gctgcgggta ccaggcgtgc
     9421 aggaaacgct gcagctcgct gcgcgagcgc gactggacca ggagctgccc gcgctcgcgc
     9481 ccgccgatgc gcgcgatggg cggcggcacc ggatcgtaca cctccaccgc caggcccagc
     9541 gccacgccgg ccgcggccgc ccgcgtcaga tagtcgtcga ccacctctcg gcggccggct
     9601 tcggcacgca acagcgcctg gtaggcatgc ggtggaaagc ccgcctggcg gcgctcgtcg
     9661 agcgcagcgc gggcgaagcc ggcgtaatcc tgccggcata ccgcgtcgta gagcggatgc
     9721 tcgggaaagt cggtctggat cagcacctcg cccggcaggt cgctgcggcc ggcacggccg
     9781 gcgacctgcg tgagctgcgc gtacagccgc tcggaggcgc ggaagtcacc gctgtacagc
     9841 gcgctgtcgg cgttcaccac gccgaccagg gtgagccgcg ggaagtcgtg gcctttggcc
     9901 agcatctgcg tgccgaccag gatgtccacc tgctggccgc ggatctgccc ttgcatggct
     9961 tcgaagctgt ggcgccggcg cgtggtgtcg cgatccacgc gcagcacgcg cgcccctgga
    10021 accgcctcca gaagcgccgc ttccaggcgc tgggtgccat ggcctgcggg catcaggtcc
    10081 tggttgccgc agcccgggca gaccgctgtg atcggctcct cgtggccgca gtaatggcag
    10141 cgcagcttgc cgtctccgag atgcagcacc agcttcgcgg agcagcgcgt gcagggcgcg
    10201 acccaggcgc aggccgggca caccagcgcc ggggcgtagc cgcggcggtt gacgaacacc
    10261 aggctctgct cgccgcgcgc cacgcgcccg cgcaatgcgt ccagcagcgg ctgcgacagg
    10321 ccgtgcgaga gtccctgtcc gcgcgtgtcg acgcagcgga tgagcggcga ggccgcgccc
    10381 ggccgatcgg gcagctcgag cagcgcatac cggccggcga tggcgttggc atagctctcc
    10441 agcgacgggg tggccgaacc cagcaccacc gggatgccgc gtagcttggc gcgcagcagc
    10501 gccaggtcgc gtgccgagta gcgcagcccc tcctgctgct tgaaggaggc gtcgtgctcc
    10561 tcgtcgacga cgatcacgcc gagctgtggc agcggagtga agacagcgag ccgcgtgccg
    10621 atcaccacgc ccacctcgcc gcgctccgcc gcgcgccagg cgcgcaggcg ctctccctcc
    10681 gccaggttgc tgtgcatggc ggcgagcggc acgccggcga aacgagtgcg gaagcgctcg
    10741 atgagctgcg gggtcaggtt gatctccggc accagcacca gcgcctgccg cccgcgctgc
    10801 agcagcgcgg ccacgctctg caggtagacc tcggtc
//
LOCUS       DASPGE010000094        19945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_849360, whole genome shotgun sequence.
ACCESSION   DASPGE010000094 DASPGE010000000
VERSION     DASPGE010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19945
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849360"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..1127
                     /gene="dnaN"
                     /locus_tag="VD791_02950"
     CDS             21..1127
                     /gene="dnaN"
                     /locus_tag="VD791_02950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HXV06961.1"
                     /translation="MTSITVQRDVVLLPLQSVSGVVERRHTLPILSNVLLELQDGVLT
                     LTATDLEIQVASRNPTGGSGAPQATTVSARKLQDILRALPEGSSVTLDIQDKRLQLKA
                     GKSKFSLQTLPSQDFPKLVAGGAASATLTLEQKDLKRLFARVHYAMAQQDIRYYLNGL
                     LLSIGPHGITAVATDGHRLALATLALELDAPKVEVIVPRKAVLELTKLLAEGEGSVKL
                     EVRENQVGFSFGVIDLITKVVDGKFPDYQKVIPSGYRKHFTLDRMGLQQALQRAAILS
                     NEKFRGVRWVLTENALRIASSNTEQEEAEEELEVIYSGEPLDVGFNVSYLLDVLANVT
                     STAVECWLGDANSSMLIALPGEPDFKYVVMPMRI"
     gene            1187..3595
                     /gene="gyrB"
                     /locus_tag="VD791_02955"
     CDS             1187..3595
                     /gene="gyrB"
                     /locus_tag="VD791_02955"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HXV06962.1"
                     /translation="MQQPSQPSEYTAESIKVLKGLEAVRKRPGMYIGDTGDGTGLHHM
                     VFEVVDNAVDEALAGYCDEIKVIIHSDNSISVIDNGRGIPTEVHPEDEGGRSTAEIVM
                     TVLHAGGKFDSNSYKIAGGLHGVGVSVVNALSEWLRLTIRRNGKVHQMEFRNGETVAP
                     LKETGLTERRGTEVHFMPSVPVFGNVEYHYDILARRLRELSFLNNGVRIELVDQRSGK
                     EELFQFAGGIKAFVEFINRTKTVLHPHIFHALREREGIGVEVAMQWNDSYQEAVLCFT
                     NNIPQRDGGTHLTALRAAMTRTLNQYIEHSDLAKRAKVETTGDDMREGLTCVLSVKLP
                     DPKFSSQTKDKLVSSEVRPVVEEVVSEALMSFLMEKPNDAKIIVAKIIEAARAREAAR
                     KAREMTRRKGVLDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRRFQAIL
                     PLKGKILNVERARFDKLLSSQEIAALITALGTGIGKDEYRPEKLRYHRIIIMTDADVD
                     GSHIRTLLLTFFYRQMPDLVERGHIYIAQPPLYKIKHGRDERYLKDDHELKDHLLKLA
                     LKDAQLFPGGDAPALTGDALAEVAREYILAEAVIERLSRRVDGAVLRALLRGVQVDLS
                     SAEAAARSAQVLVAALEGEGVLIEPQFDESTEAHRLSVRRLLHGNLRLSVVDRDLLES
                     GDYAQIRKTAGLLQGLLHEGASVQRGEHRTAVEEFPQAVEWLLEQAKSGLTIQRYKGL
                     GEMNPTQLWETTMDPASRRLLRAQIEDAIAADEIFTTLMGDVVEPRRAFIEANALGVR
                     NLDV"
     gene            complement(3600..6428)
                     /gene="topA"
                     /locus_tag="VD791_02960"
     CDS             complement(3600..6428)
                     /gene="topA"
                     /locus_tag="VD791_02960"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HXV06963.1"
                     /translation="MVESPSKAKTLKRYLGRNFEVLASYGHVRDLEAKEGAVDPEQGF
                     RMKYALVERNRKHVEAIARAAEHAEQILLATDPDREGEAIAWHLSEILRGKKGLRNRP
                     LKRVVFYEITESAVKEGVQQPRDISMPLVNAQQARRALDHLVGFNLSPLLWRKIGPSL
                     SAGRVQSPALRLIVEREQEIEAFHSREYWTIHLDSHKQKEQFSARLTQYRGAKIEQFS
                     VVSAEQQQEIVQALARAAHGAATVVKVEKKPKMRSPAAPFITSTLQQEAVRKLGMSAD
                     RAMRTAQQLYEGVDTDGGGQATSGGLITYMRTDSTNLAGEAVAEIRDYVAKNFSAPYL
                     PKAPVEYRSKSKNAQEAHEAIRPTSIFRTPDQVRAYLSADQARLYEMIWKRTVACQMA
                     PARYDTTGVDIRIGAVDIDSGGEDALFRATGQTLVFPGYIAVYQEGDDDTAEEGEERL
                     PALEEGEQIPADRIYGEQHFTLPPPRYTEASLVKKLEEYGIGRPSTYASIISTLQDRG
                     YALLDKKRFLPTDLGRLVNGFLTANFSRYVDYAFTAEMEEELDQISNGELDWVKVLRE
                     FWEPFVGTLQEKLETGRGVPLPAAEPRVSNWKEYAQRLLSEDWQQGGRTPESCPKCGK
                     QLYLQNSKRGPFVGCSGYPKCDYTRPWGELSEGPVTLGQDPETGLDVLLAKGPYGYYV
                     QLGPTLESARTKPRRASWPSNMPVSTADLDTALRLLSLPRDLGLHPETNKPVEANVGR
                     FGPYVRHDSIYKSIPKSESVYDITLARAVELLAQPKSGQGAATGRQLGPHPIDQKPVA
                     VLEGRYGPYVKHGNVNVTVPSDLDPTTLTLDDAVDLLAEKAAKELAKTGSTAAPLAAA
                     QRPRMQRPPGATGPGRQQRQAQPTAGDRRSAPAGGRGPGQNRGQHPRAAGKSAGGPGG
                     RPPPPGRNRPGRPQQRPGAKSAPRRRPG"
     gene            complement(6604..6744)
                     /locus_tag="VD791_02965"
     CDS             complement(6604..6744)
                     /locus_tag="VD791_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06964.1"
                     /translation="MALDEPEAGTEQVYLIVLMALRSREQTRDALLPDEFVAAERPGA
                     LH"
     gene            complement(6899..8023)
                     /gene="dprA"
                     /locus_tag="VD791_02970"
     CDS             complement(6899..8023)
                     /gene="dprA"
                     /locus_tag="VD791_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HXV06965.1"
                     /translation="MTGRPPTPIDTQHGDRQDAETWLRLSLIPRLGPAALRLLLKQFG
                     SPQEVLGASPAALERAAGAPIAAVIRQGAARQAVDAALAWLEQPGHHLLTLADAAYPA
                     LLLHTPDPPVVLYVTGRTELLQQPALAVVGSRSPTPRGASDAEAFACALSNAGLTIVS
                     GLAFGIDAAAHRGGLRGSASSVAVVGTGLDRVYPARHRDLAHELALRGALVSEFALGT
                     PPLPANFPRRNRILSGLARGCLVVEAALRSGSLITARQALEQAREVFAVPGSIHSPLS
                     KGCHWLIKQGAKLVESAQDVLDELRLPGAATSDAQAPAAALPPEQSALLECLGYDAVD
                     LDTVCLRGGLTPDAASAMLLALELDGYLDRLPGGLYQRRR"
     gene            complement(8066..9229)
                     /locus_tag="VD791_02975"
     CDS             complement(8066..9229)
                     /locus_tag="VD791_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXV06966.1"
                     /translation="MPLLLCLALPALAQDQDEMQPPDPAAPGAMPEDEPAAAPTEPAP
                     APAAQTMPAAPVENVPLQDDVPTRYVVVKGDTLWDISARFLKNPWKWPDVWGINRDHI
                     RNPHLIYPGDVILLDLTGATPRLRLEGVADGGISRWYGYELQRSELRPQMRSEPLGLA
                     IPTISAKAIEPFLIRPLVVDPTQVAAAPMIVANTDQRVIVSAGDTAYVTGLDQRKGPK
                     WQVYRQGRVFQDPDSKEVLGFEAVYLGDADVTGFGEVSTVHIVRAQQEIAKGDRLAIA
                     PPLQNTPYIPRAPERSVRGRVIAGADNTVFEMGTYSVLIMNRGARDGLEVGHVLGLYR
                     SEGSIPMGDNKSMPLPEQRYGLVLVFRVFNKMSYGLVMASRRPVNVLDTVRNP"
     gene            9386..9889
                     /gene="def"
                     /locus_tag="VD791_02980"
     CDS             9386..9889
                     /gene="def"
                     /locus_tag="VD791_02980"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXV06967.1"
                     /translation="MALLPILHYPDERLHRVAAPVQEINDRIRQLVRDMAETMYSAPG
                     IGLAATQVDVHEQVIVIDISETRDRLQVFINPELLDSRGQAECEEGCLSVPGIYEKVT
                     RAEWIKVRARDADGKSFTLEADGLLGVCIQHEMDHLKGKVFVEYLSRLKQSRIIGRLK
                     KQQRQAM"
     gene            9889..10824
                     /gene="fmt"
                     /locus_tag="VD791_02985"
     CDS             9889..10824
                     /gene="fmt"
                     /locus_tag="VD791_02985"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009972654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HXV06968.1"
                     /translation="MRLVFAGTPAFARSALQALLDAGHEIALVLTRPDRPAGRGLKPR
                     PSEVKALALERGLPLHQPATLLGDDACAPLQAAAARTMVVAAYGLILPQSVLDLYALG
                     CINIHASLLPRWRGAAPIQRALLAGDAQTGISIMRMEAGLDTGPVYLTRALPIGARDT
                     AQTLHDKLAVLGGECIVQALPEIAAGTLIAAPQPEAGVTYAHKIGKDEAELDWRRSAA
                     ELDRQVRAFVPFPVATTRLRGEVLRVWAALPLDGRCGPPGTALEITADGIVVACGAGA
                     LCLQRLQRAGGKQLPAREFLLGARLAVGERLGPST"
     gene            10835..12133
                     /gene="rsmB"
                     /locus_tag="VD791_02990"
     CDS             10835..12133
                     /gene="rsmB"
                     /locus_tag="VD791_02990"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HXV06969.1"
                     /translation="MTEVQGRAALALLAVLRGRSLTAVLDEIRSRNPRLEANERAAIQ
                     DLAFGSCRWLGTLRAVLAQLLRAPLTDAEVEALLLVALYQLHWTRAAQHAVVDGAVRT
                     CVALDKPAAKGLVNGVLRNFLRRREALMAQAQATSEGRFSYPQWWIDEARAAWPAQYA
                     EILDAGNLHPPMTLRVNRRKGDAQSYRATLARAGIDTEPLGSGALGAAALLLAKPMPV
                     QALPGFEQGLVSVQDLAAQYAAPLLDLASGQRVLDACAAPGGKTAHILELCDVELLAL
                     DRDAQRLDRVRGNLDRLGLKARLQAADAADARAWWDGRPFDRILLDAPCTGSGVVRRH
                     PDAKWLRRPGDLAQLAAEQSRLLQGLWHTLAADGKLLYVTCSVFPAETESRIAAFLGS
                     HGDASRLGLAGLPAGGQLLPDMRHDGFFYALLQKRPAPAD"
     gene            12140..12715
                     /locus_tag="VD791_02995"
     CDS             12140..12715
                     /locus_tag="VD791_02995"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HXV06970.1"
                     /translation="MKLLFRRRAWVRDLLAILLGALLATSARGAVLIKSAELRPSEAG
                     YTLEATYDVGLTPTLEEALERGLSLTFVTEFELVYPRWWTFNLWNRTVSEFSTQHRLS
                     YNALARQYRLAFGSLHQSFDTVVEALAVVGRVRFAPAVRFPEVEPGAVYLAALRLRLD
                     TSELPKPLQIDALASNDWSLSSDWYRWTFQP"
     gene            12712..14910
                     /locus_tag="VD791_03000"
     CDS             12712..14910
                     /locus_tag="VD791_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV06971.1"
                     /translation="MSVSIHSVRHSLLSRGMKYLIALILMLGALMLYLLSSVSADTQL
                     FASDLRIMLLLGVCLVLGLMALVGYQLLALRRRLRAGLFGSKLTLRLVLLFALVAVLP
                     GALVYAVSVQFLNKSIESWFDVRVDKALEGGLSLGRTTLDNMLRDLTRKAESMAMSLD
                     DVSRSQLVPALNALREQAAVQEATVFRADGGILAFSVSEGTGLMPTALPPSAARQILL
                     QQPYREIDPDAPQGLSLVAVVPLNRLSGDTRALQLVQLVPQQLARDASTVQAMYRDYQ
                     ELALSRSGLKRLYGLSLTLSLLLALLSALVLAILFSERLSAPLVLLAAGTRAVAQGDF
                     SQRHPVRSHDELGVLTESFNTMTRQLAEARAAALGNQAQLEAAKAYLESILAKLSAGV
                     LSFDSERRLRSANPSAGEILGVDLQPLIGERVEDWPERDVRLAEIGRAVLESILADGT
                     EAWEKQLEVAGGSKVLLMRGSALPGDVEGGFVVVFDDITRLLQAQRYAAWGEVARRLA
                     HEIKNPLTPIQLSAERLEHRLAEKLQHTDAEMLARSTQTIVKQVAQLKGMVDAFSQYA
                     RTPEMQLQPLDLNQLVREVLPLYDSQRPRVLLELHEGLPPVHGDPARLRQVIHNLLQN
                     AEQALVESSGARITLRTLPSGAWVRLTVLDNGPGFPPEMLARAFEPYVTTKAKGTGLG
                     LAIVKKIVEEHNGSIEIANPKGGGAQVSILLPVATQAAQPERAPVNARVG"
     gene            14917..15311
                     /locus_tag="VD791_03005"
                     /pseudo
     CDS             14917..15311
                     /locus_tag="VD791_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179708.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            15307..15723
                     /locus_tag="VD791_03010"
                     /pseudo
     CDS             15307..15723
                     /locus_tag="VD791_03010"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            15994..16176
                     /locus_tag="VD791_03015"
                     /pseudo
     CDS             15994..16176
                     /locus_tag="VD791_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            16279..18309
                     /gene="tkt"
                     /locus_tag="VD791_03020"
     CDS             16279..18309
                     /gene="tkt"
                     /locus_tag="VD791_03020"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXV06972.1"
                     /translation="MPEPSSRKTYSMATRQELANALRALAMDAVQKANSGHPGMPMGM
                     AEIAEVLWNHHLRFNPANPNWPDRDRFVLSNGHGSMLLYALLHLTGYDLPMDELRRFR
                     QLHSKTPGHPEHGLAPGVETTTGPLGQGLANAVGMALAERALAAEFNRPGSEIVNHHT
                     YVFVGDGCLMEGISHEACSFAGTLGLGKLIAIYDDNGISIDGHVQAWFTDDTPRRFQA
                     YGWHVLPNVDGHSFEAVHTAIESAKAAADRPSLICCKTVIGKGAPNKADSHDAHGSPL
                     GEKEVAATRENIGWRHPPFEIPADVYQGWDARVKGAGLEKLWRNRFAEYQRAHPALAA
                     EFERRMAGDLPAAWREHAAAYLARTVEKAESIATRKASQNAIEGLAPKLPELIGGSAD
                     LAASNLTMWSGSRPVRRADGGNYVFYGVREFGMTAIMSGLALHGGFIPYGGTFLTFSD
                     YSRNALRMAALIKVRSIFVFTHDSIGLGEDGPTHQPVEHAATLRYIPNMEVWRPCDSV
                     ETAVAWTAAVERKDGPTALLLSRQNLPFVKRSAAQLALISRGGYVLAEAQGGTPAVAL
                     LATGSEVALALEAQKALSEQGVAARVVSMPCTGMFDRQDAAYRQSVLPQGLRRVAIEA
                     GVSDFWRKYVGLEGGVVGIDRFGESAPAGELFKYFAFTPGHVVETVKRVLHG"
     gene            18320..19288
                     /locus_tag="VD791_03025"
     CDS             18320..19288
                     /locus_tag="VD791_03025"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HXV06973.1"
                     /translation="MGDAPFWLLADIGGTNVRFALLPPRSDQPQRERSLRCADYPGLE
                     QAARAYLADAGSPRVTEAAIDVATAVTGDLVKLTNSPWAFSIEQTRRQLGLARLLVLN
                     DFTALALSLPLLNATELRQVGRGQALAGKAIGLIGAGTGLGVSGLLPCGARWVPLEGE
                     GGHVAFSPVDAREDAVLAVLRERFGHVSAERVLSGPGVVNVYQALCRIDHVPTRALEP
                     EQIAQAARSGADPQCLEALAMFCGALGTAAGNLAITLGARGGVYIGGGIVPKLGDYFE
                     RSSFRARFEHKGRFDSYVAGIPTFVIHADYPALRGLAVALNEDPNS"
     gene            complement(19285..>19945)
                     /locus_tag="VD791_03030"
     CDS             complement(19285..>19945)
                     /locus_tag="VD791_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HXV06974.1"
                     /translation="PTLFLFDEPLSNLDAKLRVEMRTEIKLLHERLAATIVYVTHDQI
                     EAMTLGDRIAVMKEGVVRQLATPQEVYDNPADLFVAGFIGSPSMNFIRCPVTQSDGRL
                     GVQLHEGSERFFFPAPEPMQKGLGAWNGREVMLGIRPEQITDKTPYSGQNPNVVTRVA
                     KIEVVQPTGPDTLVLIHLNDTPVTCRVHPEAGARPGQPATLMFDLSKLVFFDPQTEKR
                     I"
BASE COUNT         3062 a         6813 c         6931 g         3139 t
ORIGIN      
        1 atctataacc aggaagaaaa atgacatcaa taactgtaca acgcgatgtc gtgctcctgc
       61 cgctgcagtc ggtcagcgga gtggtcgagc gccgtcatac gctgccaatc ctgtccaatg
      121 tcctgctcga gttgcaggat ggcgtgctca cgctcaccgc gaccgatctg gagatccaag
      181 ttgcttcgcg caacccgacc ggcggcagtg gagcgccgca ggcgaccacg gtgtcggcgc
      241 gcaagctgca ggatatcctg cgcgccctgc cggaaggctc cagcgtgacg ctggacatcc
      301 aggacaaacg cctgcagctc aaggccggca agagcaagtt ctcgctgcag acgctgccct
      361 cgcaggattt ccccaagctg gttgccggtg gcgcagcgag cgccacgctg acgctcgagc
      421 agaaggatct caagcgcctg ttcgcgcgcg tgcactacgc catggcgcag caggatatcc
      481 gctattactt gaacggtttg ctgctgtcga tcgggccgca cggcatcacc gcggtggcca
      541 ccgacggtca ccgcctggcg ctggctaccc tggcgctgga gctggacgcg cccaaagtgg
      601 aggtgatcgt gccgcgcaaa gccgtgctcg aactcaccaa gctgctggcc gagggggaag
      661 gctcggtgaa gctggaagtg cgcgagaacc aggtgggctt cagcttcggg gtgatcgacc
      721 ttatcaccaa ggtggtggac ggcaagttcc ccgactatca gaaggtcatc cccagcggct
      781 accgcaagca cttcacactc gaccggatgg ggctgcagca ggcgctgcag cgcgcagcga
      841 tcctgtcgaa cgagaaattc cgcggcgtgc gctgggtgct gaccgagaac gccttgcgca
      901 tcgccagcag caacaccgag caggaagaag ccgaggaaga gctcgaggtg atctacagcg
      961 gcgagccgct cgacgtgggc ttcaacgtga gctacctgct cgacgtgctg gccaacgtca
     1021 ccagcactgc ggtcgagtgc tggctaggcg acgccaactc cagcatgctg atcgcgctgc
     1081 ccggcgaacc ggacttcaag tacgtggtca tgccgatgcg catctgatgc gcggcgagcc
     1141 gaaccaaccc atgtcttcga tcccacgaga aaaccctgcg acctccatgc agcagccgtc
     1201 ccagccgtcc gaatacaccg cggaaagcat caaagtcctg aaggggctgg aagcggtgcg
     1261 caagcgtccc ggcatgtaca tcggcgacac cggcgacggc accgggttgc atcacatggt
     1321 gttcgaggtg gtggacaacg cggtggacga ggccctggcc ggctattgcg acgagatcaa
     1381 ggtcatcatc cactcggaca actcgatcag cgtcatcgac aacggccgcg gcatccccac
     1441 cgaagtgcac cccgaagacg agggcggccg ctcgaccgcc gagatcgtga tgacggtgct
     1501 gcacgccggc ggcaagttcg acagcaactc ctacaagatc gcgggcggcc tgcacggcgt
     1561 aggcgtgtcg gtggtcaatg cgctgtccga atggctgcgc ctgaccatcc gccgtaacgg
     1621 caaggtgcat cagatggagt tccgcaacgg cgagacggtc gcgccgctga aggaaaccgg
     1681 cctgaccgag cgccgcggca ccgaggtgca cttcatgccg agcgtgccgg tcttcggcaa
     1741 cgtcgaatac cactacgata tcctggcgcg ccggctgcgc gagctgtctt tcctcaacaa
     1801 cggcgtgcgc atcgagctgg tggaccagcg cagcggcaag gaggagctgt tccagttcgc
     1861 cggcggcatc aaggccttcg tcgagttcat caaccgcacc aagacggtcc tgcacccgca
     1921 tatcttccac gcgttgcgcg agcgcgaggg catcggcgtc gaggtggcga tgcagtggaa
     1981 cgactcgtac caggaggcgg tgctgtgctt caccaacaac atcccgcagc gcgacggcgg
     2041 cacgcacctc acggcgctgc gcgccgccat gacgcgcacc ttgaaccagt acatcgagca
     2101 cagcgacctc gccaagcgcg ccaaggtgga gaccaccggc gacgacatgc gcgagggcct
     2161 gacttgcgtg ttgtcggtga agctgcccga cccgaagttc tcctcccaga ccaaggacaa
     2221 gctggtctcc tccgaagtgc gcccggtggt ggaggaggtg gtgtccgagg cgctgatgtc
     2281 gttcctgatg gagaagccca acgacgccaa gatcatcgtc gccaagatca tcgaggccgc
     2341 gcgtgcgcgc gaggcggcgc gcaaggcgcg cgagatgacc cggcgcaagg gcgtgctcga
     2401 cggcctggga ctgcccggca agctggccga ttgccaggag aaggacccgg cgttatccga
     2461 gctgtacctg gtggagggcg actccgccgg cggctcggcg aagcaaggcc gggaccggcg
     2521 cttccaggcg atcctgccgc tgaagggcaa gatcctgaat gtcgagcggg cgcgcttcga
     2581 caagctgctg tcgtcgcagg agatcgccgc cctgatcacc gcgctcggca ccggcatcgg
     2641 caaggacgaa taccggcccg aaaaactgcg ctaccaccgc atcatcatca tgaccgacgc
     2701 ggacgtcgac ggctcccaca tccgcacgct gttgctgact ttcttctacc ggcaaatgcc
     2761 ggatctggtg gagcgcggcc acatctacat cgcgcagccg ccgctgtaca agatcaagca
     2821 cgggcgcgac gagcgctatc tgaaggacga tcacgagctg aaggaccacc tgctcaagct
     2881 cgcgctcaag gacgcgcagc tgttccccgg cggggacgcc ccggcgttga ccggcgatgc
     2941 cctggccgag gtcgcgcgcg agtacatcct ggccgaggcg gtgatcgaac gcttgtcgcg
     3001 ccgggtcgac ggtgcggtgc tgcgcgcgct gctgcgcggc gtgcaggtgg acctgtccag
     3061 cgcggaagcc gcggcgcgca gcgcccaggt gctcgttgct gcgctggagg gcgaaggcgt
     3121 gctgatcgag ccgcagttcg acgagagcac cgaagcgcac cgcctgagcg tgcgtcgcct
     3181 gctgcacggc aacctgcgct tgagcgtggt cgaccgggac ctgctggaga gcggcgacta
     3241 cgcgcagatc cgcaagaccg cgggcctgct gcagggcctg ctgcacgagg gcgcgagcgt
     3301 gcagcgcggc gagcaccgca cggcggtcga agagtttccg caggcggtgg aatggctgct
     3361 cgagcaggcc aagagcgggc tcaccatcca gcgctacaag ggcctgggtg aaatgaatcc
     3421 gacgcagctt tgggagacca ccatggatcc cgcctcgcgg cggctgctgc gcgcgcagat
     3481 cgaggacgcc atcgccgcgg acgagatttt caccacgctg atgggcgacg tggtcgaacc
     3541 gcgccgcgcc ttcatcgagg ccaacgcgct gggcgtgcgc aacctcgacg tgtagtcggc
     3601 tagcccgggc ggcggcgcgg cgcggacttg gcgccggggc gctgttgcgg gcgcccgggt
     3661 cgattgcggc ccggcggcgg tggacgcccg cctggcccgc ctgcgctctt gcccgccgct
     3721 cgcgggtgct ggccgcggtt ctggccgggg ccacgcccgc cggcgggcgc ggatctacga
     3781 tcgcccgcag tcggttgcgc ctggcgctgc tgtctgcctg gaccggtcgc accggggggc
     3841 cgctgcatgc gcggacgctg cgcagccgcc agcggtgcgg cggtgctgcc ggtcttggcg
     3901 agctccttcg ccgctttctc cgcgagcagg tcgaccgcgt cgtcgagcgt gagcgtggtc
     3961 ggatccaggt ccgagggcac ggtgacgttg acgttgccgt gcttcacgta ggggccatag
     4021 cgcccttcca gcaccgccac cggcttctga tcgatgggat gcggaccgag ctggcgtcct
     4081 gtggcggcgc cctgtccgct cttcggctgc gccagcagct ccaccgcgcg ggcgagcgtg
     4141 atgtcgtaca cgctctcgct cttggggatc gacttgtaga tgctgtcgtg cctgacgtac
     4201 ggcccgaagc ggccgacgtt ggcttccacc ggcttgttgg tttcggggtg caggcccagg
     4261 tcgcgcggca gcgacagcaa cctgagcgcg gtgtcgagat cggccgtgga caccggcatg
     4321 ttgctcggcc acgaggcgcg gcgcggcttg gtgcgggcgc tttccagcgt cggaccgagc
     4381 tgcacgtagt agccgtaggg acccttggcc agcagcacgt cgagcccggt ctcgggatcc
     4441 tgccccagcg tcaccggacc ctcgctcagc tcgccccagg gccgcgtgta atcgcacttg
     4501 ggatagccgc tgcagcccac gaacggcccg cgcttgctgt tctgcaggta gagctgcttg
     4561 ccgcacttcg ggcagctctc cggcgtgcgg ccgccctgct gccagtcttc gctcagcagc
     4621 cgctgcgcgt actccttcca gttgctcacg cgcggctcgg cggccggcag cggcacgccg
     4681 cggccggtct ccagcttctc ctgcagcgtg ccgacgaagg gctcccagaa ctcgcgcagc
     4741 accttgaccc agtccagctc gccgttggag atctggtcga gctcctcctc catctccgcg
     4801 gtgaaggcgt agtccacgta gcggctgaaa ttggcggtga gaaagccgtt caccagtcta
     4861 ccgagatcgg tgggcaggaa gcgcttcttg tccagcagcg cgtagccgcg gtcctgcagc
     4921 gtcgagatga tgctggcata ggtcgagggg cggccgatgc cgtactcctc gagcttcttc
     4981 accaggctcg cctcggtgta acgcggcggc ggcaaggtga agtgctgctc gccgtagatg
     5041 cgatccgcgg ggatctgttc gccctcttcg agtgccggca gcctctcctc gccttcctcc
     5101 gccgtgtcgt cgtccccctc ctggtacacc gcgatgtagc cggggaacac gagcgtctgc
     5161 ccggtggcgc ggaacagggc gtcctcgccg ccgctgtcga tgtcgacggc gccgatgcga
     5221 atatcgacgc cagtggtgtc gtagcgcgcg ggcgccatct ggcaggccac ggtgcgcttc
     5281 cagatcatct cgtagagccg cgcctgatcg gccgagaggt aggcgcgcac ctggtccggc
     5341 gtgcggaaga tggaggtcgg gcggatggcc tcgtgcgcct cctgcgcgtt cttcgacttg
     5401 ctgcggtact ccaccggcgc cttcggcaag tacggcgcgc tgaagttctt cgccacgtaa
     5461 tcgcggatct ccgccaccgc ttcgccggcg aggttggtgg agtcggtgcg catgtaggtg
     5521 atcaagccgc cggaggtcgc ctgaccgccg ccgtcggtgt ccacgccctc gtagagctgc
     5581 tgcgccgtgc gcatcgcccg gtccgccgac ataccgagct tgcgcaccgc ttcctgttgc
     5641 agggtggagg tgatgaacgg cgccgccggc gagcgcatct tgggcttctt ctcgaccttg
     5701 accacggtgg cggccccgtg cgccgcgcgc gcgagcgcct gcacgatctc ctgctgctgc
     5761 tcggcgctca ccaccgagaa ctgttcgatc ttggcgcccc ggtactgggt caggcgcgcg
     5821 ctgaactgct ccttctgctt gtggctgtcc aggtggatgg tccaatactc ccggctgtgg
     5881 aacgcctcga tctcctgttc gcgttccacg atcagacgca gcgccgggct ctgcacccgg
     5941 cccgccgaca ggctggggcc gatcttgcgc cacagcaggg gcgagagatt gaagcccacc
     6001 aggtgatcga gggcgcggcg cgcctgctgc gcgttgacca gcggcatgga gatgtcgcgc
     6061 ggctgttgca cgccttcctt caccgcggac tcggtgatct cgtagaacac cacgcgcttc
     6121 agcgggcgat tgcgcaggcc cttcttgccg cgcaggatct cggacaggtg ccaggcgatc
     6181 gcttcgcctt cgcgatccgg gtcggtggcg agcaggatct gctcggcgtg ctcggccgcg
     6241 cgcgcgatgg cctccacgtg cttcctgttg cgctccacca gcgcgtactt catccggaag
     6301 ccttgctccg gatcgacggc gccttccttc gcctccaggt cgcgcacgtg gccgtaggag
     6361 gcgagcactt cgaaattgcg cccgaggtac ctcttgagcg tcttggcctt ggagggcgat
     6421 tcaaccacga gaagactgat cggcatgcgg gcccttgggc aataaaatgc ggcggttcag
     6481 atcgccggcg tcgacccggc aagcgggtcg cgatacggcg tgcaagatcg acgaaagcgt
     6541 tgctgccgcg aggacgccac gcggtatcag cgacacaagc gcggccttgc cgcggtgcgg
     6601 atactagtgc aacgcgcctg gacgctcggc agcgacaaac tcgtccggga gcagcgcgtc
     6661 ccgcgtctgc tcccggctcc gaagcgccat cagcacgatg aggtagacct gctcggtgcc
     6721 ggcttccggc tcgtccaggg ccacgatgcg atcgacggct cctgtgacga ttcgagcacg
     6781 cccgcggcct ccaaacactg caggaatccg cgtccgccgg gatggatctt gcgcatctcc
     6841 ggggctgcgt aacgccggac actggcacct gcaggtaagc gttcgctgcg ccgcagactc
     6901 agcgtctgcg ctgatacagc ccaccgggca gccggtcgag atacccgtcc agttccagcg
     6961 ccaacagcat ggcggaagcc gcatcgggcg tcaagccgcc gcgcaggcac accgtgtcca
     7021 agtccaccgc atcgtaaccc aagcactcga gcagcgccga ctgctcgggc ggcaacgctg
     7081 ctgccggcgc ttgcgcgtcg ctggtcgccg cgcccggcag gcgtaactcg tccagtacat
     7141 cctgggccga ctccaccagc ttcgcccctt gcttgatcag ccagtggcag cccttcgaca
     7201 gcggcgagtg aatcgatccg ggcacggcga acacctcgcg ggcctgctcc agggcctggc
     7261 gtgcggtgat caacgatccg ctgcgcagcg ccgcctccac caccaggcaa ccgcgcgcga
     7321 gcccgctgag gatgcgattg cggcgcggga agttggccgg gagcggtggc gtgccgagcg
     7381 cgaactcgga taccagcgcg ccgcgcagcg ccagctcgtg cgccaggtcg cgatggcgtg
     7441 cgggatagac gcggtccagg ccggtaccga ccactgccac gctggacgcg ctcccgcgaa
     7501 gcccgccgcg atgcgccgcc gcgtcgatgc cgaaagccag gccgctgacg atggtgagcc
     7561 cggcgttgct cagcgcgcag gcgaacgcct cggcgtcgga agcaccgcgc ggcgtggggc
     7621 tgcggctgcc gaccaccgcc agcgcaggct gttgcagcag ctccgtgcgc cccgtcacgt
     7681 acagcaccac aggcggatcg ggcgtgtgca gcagcagcgc cggataggcg gcgtcggcga
     7741 gcgtcagcag atggtgtccg ggttgctcga gccaggcgag ggcggcgtcc acggcttggc
     7801 gcgccgcgcc ctggcggatc actgcggcga tcggcgcgcc ggccgcgcgc tccagcgccg
     7861 cgggcgaagc gcccagcacc tcctggggcg agccgaactg tttcagcagc agccgcagcg
     7921 ccgccggacc gagcctggga atcaggctga ggcgcagcca ggtctctgcg tcctgccggt
     7981 ctccgtgctg agtatcgatg ggtgtcggcg gccgccctgt caagtcatcg gttcccggcg
     8041 gcagggaccg aatgaaatat ccgggctagg gattgcgcac cgtgtcgagc acgttcaccg
     8101 gccggcgcga cgccatcacc agcccgtagg acatcttgtt gaacacacgg aacaccagca
     8161 ccaggccgta gcgctgctcc ggcagcggca tcgacttgtt gtcgcccatc gggatcgagc
     8221 cttccgagcg gtacaggccg agcacgtgac cgacctcgag cccgtcgcgg gcgccgcgat
     8281 tcatgatcag caccgagtag gtgcccatct cgaacaccgt gttgtccgca cccgctatca
     8341 cgcgtccgcg cacggagcgc tccggcgcgc gcgggatgta gggcgtgttc tgcagcggcg
     8401 gggcgatcgc gaggcgatcg cccttggcga tctcctgctg cgcgcgcacg atgtgcaccg
     8461 tgctgacctc gccgaagccg gtcacgtcgg cgtcgcccag atacaccgcc tcgaaaccga
     8521 gcacctcctt gctgtccggg tcctggaaca cgcgtccctg ccggtacacc tgccatttgg
     8581 gccccttgcg ctgatccagc ccggtgacat aagccgtgtc gccggcggag actatcacgc
     8641 gctgatcggt gttggcgacg atcatcggcg cggccgccac ttgagtcgga tcgaccacca
     8701 gcgggcggat caggaacggc tcgatcgcct tggcggagat ggtcgggatg gccaggccga
     8761 gcggctcgct gcgcatttgc ggccgcagct cggaccgctg cagctcgtag ccgtaccagc
     8821 gcgagatgcc gccgtcggcc acgccttcca gccgcagccg cggcgtggcg ccggtcaagt
     8881 ccaggaggat cacgtctccc ggatagatca ggtgcggatt gcggatgtgg tcgcggttga
     8941 tcccccacac gtcgggccac ttccaggggt tcttgaggaa gcgcgcggag atgtcccaca
     9001 gcgtgtcgcc cttgaccacc acgtagcggg taggaacatc gtcctgcagg ggcacgttct
     9061 cgacgggcgc agccggcatg gtctgcgccg ccggggcggg cgcgggttcg gtcggtgcgg
     9121 ccgcgggttc gtcttccggc atcgcgccgg gcgccgcggg gtcgggcggc tgcatttcgt
     9181 cctgatcctg agccagggcc ggcagcgcca ggcagagcag caggggcaca gccgatataa
     9241 ttccgcgcat cgttgtctcc cgagacgggt cggggaaggg ctccccgtcg accctggaac
     9301 ggtccgcaag aactgcggcc ttagtgcgta acatcaataa cttccctcgc attctgcacg
     9361 ttacttatca gcttggcaag cgcaaatggc gttgctaccg attctgcatt acccggacga
     9421 gcggctgcac cgcgtggccg ctccggtcca ggagattaac gaccggatcc gtcaactcgt
     9481 tagggacatg gcggagacca tgtactcggc cccgggcatc gggctggccg cgacgcaggt
     9541 cgacgtgcac gagcaggtga tcgtgatcga catctccgaa acgcgcgatc ggttgcaggt
     9601 gttcatcaat cccgagctcc tcgacagccg cgggcaggcc gagtgcgagg agggatgcct
     9661 ttcggtgccc ggtatctacg agaaagtcac gcgcgccgag tggatcaagg tgcgcgcccg
     9721 ggacgccgac ggcaagtcct tcacgctgga agccgacggc ctgctggggg tgtgcatcca
     9781 gcacgaaatg gaccacctca agggcaaggt cttcgtggag tacctgtcgc ggctgaagca
     9841 gtcgcgcatc atcggccgcc tgaagaagca gcagcgccag gccatgtgat gcggctcgtg
     9901 ttcgccggca cgccggcctt cgcgcgcagc gcactgcagg cgctgctcga cgccggccac
     9961 gagatcgccc tggtgctcac tcgaccggac cggccggcgg gccgcggcct gaaaccgcgg
    10021 ccctcggagg tcaaggcgct cgcgctcgag cgcggtctgc ccttgcatca gccggcgacg
    10081 ctgctcggcg acgacgcgtg cgcgccgctg caggccgccg ccgctcgcac catggtggtc
    10141 gccgcgtatg ggctgatcct gccgcagtcc gtgctcgatc tttatgcgct gggctgcatc
    10201 aacatccacg cttcgctgct gccgcgctgg cgcggcgccg cgccgattca gcgcgccctg
    10261 ctcgccggcg acgcgcagac cggcatcagc atcatgcgca tggaggccgg cctggacacc
    10321 ggcccggtgt atctgacgcg ggcgctgccg atcggtgcgc gcgacaccgc gcaaacgctg
    10381 cacgacaagc tcgccgtcct gggcggggag tgcatcgtgc aggccttgcc ggagatcgca
    10441 gccggcacgc tcatcgccgc gccgcagccc gaggccggcg tgacctacgc gcacaagatc
    10501 ggcaaggacg aggccgagct cgactggcgg cgcagcgcgg cggagctgga ccgtcaggtg
    10561 cgcgcgttcg tgccctttcc cgtggccacc acgcgcttgc gtggggaggt gttgcgcgtc
    10621 tgggcggcgc ttccgctcga cggtcgttgc ggcccgcccg gaacggcgct ggaaatcacc
    10681 gcggacggca tcgtagtagc ctgcggcgcg ggcgcgctgt gtctgcagcg cctccagcgc
    10741 gcgggcggca agcagctgcc ggcgcgcgag ttcctgctcg gcgcgcggct cgccgtcggc
    10801 gagcggcttg gcccgtccac gtgaccgaga tcctgtgacc gaggttcagg gccgcgccgc
    10861 gctcgcgctt ctggcggtcc tgcgcggccg cagcctcacg gccgtgctcg acgagatccg
    10921 gtcgcgcaac ccgcgcctgg aagcgaacga gcgcgccgcg atccaggatc tcgccttcgg
    10981 cagctgccgc tggctcggca cgttgcgcgc ggtgctggcg cagctgctgc gcgcgccgct
    11041 aaccgacgcg gaggtcgaag cgctgctgct ggtcgccctg tatcagctgc actggacccg
    11101 ggccgcgcag cacgcggtgg tggacggggc ggtgcgcacc tgcgtggcgc tggacaagcc
    11161 ggccgcgaaa ggtctggtga acggcgtgct gcgcaatttc ctgcgccgcc gcgaggcgct
    11221 gatggcacag gcgcaggcca cctcggaggg tcgcttctcc tacccgcaat ggtggatcga
    11281 cgaggccagg gccgcctggc cggcacagta cgcggaaatt ctggacgcgg gcaatctgca
    11341 tcctccgatg acgctgcgcg tgaaccgccg caaaggcgac gcgcagtcct atcgggcgac
    11401 gctggcgcgg gcgggcatcg ataccgagcc gctgggctcg ggggcgctgg gtgccgcggc
    11461 gctgctgctg gcgaaaccga tgccggtgca agcgctgccg ggcttcgagc agggcctggt
    11521 gtcggtccag gacctcgcgg cgcagtacgc agcgccgctg ctggacctcg cttccggcca
    11581 gcgggtgctg gacgcgtgcg cggcgccggg cggcaagacc gcgcacatcc tcgaactgtg
    11641 cgacgttgag ctgctcgcgc tcgatcgcga cgcgcaacgc ctcgatcggg tccgcggcaa
    11701 cctggaccgt ctcggcctga aagcgcggct gcaggcggcg gacgccgcgg atgcgcgcgc
    11761 ctggtgggac ggccgcccgt tcgaccgcat tctgctcgat gcgccctgca ccggctcggg
    11821 agtggtgcgc cgccatccgg atgccaaatg gctgcgccgg ccgggcgacc tcgcgcagct
    11881 tgccgccgag cagagccgcc tgctgcaggg cctgtggcac acccttgccg ctgatggtaa
    11941 attgctctat gtgacctgct cggtcttccc ggccgaaacc gaatcgcgca tcgccgcctt
    12001 cctgggatcc cacggcgacg cgtcgcggct cgggcttgcc gggctgcccg ccggggggca
    12061 gctgctaccg gacatgcgcc acgatggctt cttctacgct ctgcttcaga agcgccccgc
    12121 gcctgccgat tagccggcaa tgaaactcct gttccgccgg cgcgcctggg tgcgcgatct
    12181 ccttgcgatc ctgctcggcg cgctgctggc gacgagcgcg cggggcgccg tgctgatcaa
    12241 gtcggccgag ctgcggccct ccgaagcagg ctatacgctg gaggcgacct acgatgtcgg
    12301 cctgacgccg acgctcgagg aggcgctgga gcgcggactg tccctgacct tcgtcaccga
    12361 gttcgagctg gtgtacccgc gctggtggac cttcaacctg tggaaccgga ccgtctccga
    12421 gttcagcacg cagcatcgcc tgtcctacaa cgcgctcgcg cgccagtacc ggctcgcctt
    12481 cggttctctg caccagagct tcgacaccgt ggtcgaagcg ctcgccgtgg tcgggcgcgt
    12541 gcgcttcgcg ccagcggtgc gctttccgga ggtggaaccc ggcgcggtct atctggcggc
    12601 gctgcgcttg cgcctggaca cctccgagct gcccaagccg ctgcagatcg acgcgctggc
    12661 gtcgaacgac tggagtctgt cttccgactg gtaccgctgg accttccagc catgagcgtt
    12721 tcgatccaca gcgtgcgcca ttcgctgctg tcgcgcggca tgaagtacct gatcgccttg
    12781 atcctcatgc tgggcgccct gatgctctat ctgctgtcca gcgtcagcgc ggatacgcag
    12841 ctgttcgcca gcgacctgcg catcatgttg ctgctcggcg tgtgcctggt gctggggctg
    12901 atggcgctgg tgggctatca gctgctggcg ctgcggcgcc ggctgcgcgc cggcctgttc
    12961 ggctccaagc tcactctgcg gctggtgctg ctgttcgcgc tggtggcggt gctacccggc
    13021 gcgctggtgt atgccgtgtc ggtgcagttc ctgaacaaga gcatcgaatc ctggttcgac
    13081 gtgcgcgtgg acaaggccct cgaaggcggt ttgagcctgg gccgcacgac gttggacaac
    13141 atgctgcgcg acctcacgcg caaggccgag tcgatggcga tgtcgctcga cgacgtgtcc
    13201 aggtcgcagc tcgtgcccgc gctcaacgcg ctgcgcgagc aggcggcggt gcaagaagcc
    13261 accgtgttcc gcgcggacgg cggcatcctt gccttttcgg tgagcgaggg cacggggctg
    13321 atgcccaccg cgctcccgcc ttcggctgcg cggcagatcc tgctgcagca gccttaccgt
    13381 gagatcgatc cggatgcgcc gcaaggactg agcctggtgg cggtggtgcc gcttaacagg
    13441 ctctccggcg atacgcgcgc gctgcagctg gtgcagctgg tgccgcagca gctggcgcgc
    13501 gacgcgagca cggtgcaggc gatgtaccgg gactaccagg agctggcgct ctcgcgcagc
    13561 gggctgaagc gcctgtacgg gctttcgctc acgctgtccc tgctgctggc gctgctctcg
    13621 gcgctggtgc tggcgatcct gttcagcgag cgcctgtcgg cgccgctggt gctgctggcc
    13681 gccggcaccc gcgccgtggc gcagggcgac ttcagccagc gccatccggt gcgcagccac
    13741 gacgagctgg gcgtgctcac cgaatccttc aacaccatga cgcgccagct cgcggaggcg
    13801 cgcgcggcgg cgctcggcaa ccaggcgcaa ctggaggcgg cgaaggccta cctggagagc
    13861 attctggcga agctctcggc cggcgtgctg tcgttcgaca gcgagcggcg cctgcgctcg
    13921 gccaacccga gcgcaggcga gatcctgggc gtggacctgc agccgttgat cggcgagcgc
    13981 gtggaagact ggccggagcg cgacgtacgc ctggcggaga tcgggcgcgc ggtgctggaa
    14041 tcgatcctgg cggacggcac ggaagcctgg gagaagcagc tggaggtggc gggcggcagc
    14101 aaggtgctgc tgatgcgcgg gagcgcgctg ccgggggacg tggagggcgg cttcgtggtg
    14161 gtgttcgacg acatcacacg gctgctgcag gcgcaacgct acgccgcctg gggcgaggtc
    14221 gcgcgccggc tcgcgcacga gatcaagaac ccgctcacgc cgatccagct gtccgccgaa
    14281 cgcctggagc atcgcctagc ggagaagctg cagcacaccg acgccgagat gctggcgcgc
    14341 tcgacccaga ccatcgtcaa gcaggtagcg cagctgaagg ggatggtgga cgccttcagc
    14401 cagtacgcgc gcaccccgga gatgcagctg cagccgctcg acttgaacca gctggtgcgc
    14461 gaggtgctgc cgctgtacga cagccagcgc ccgcgcgtgc tgctggagct gcacgagggc
    14521 ctgccgccgg tgcacggcga cccggcgcgg ctgcgccagg tgatccacaa tctgctgcag
    14581 aacgccgagc aggcgctcgt cgaatcgagc ggcgcgcgca tcaccttgcg cacgctgccc
    14641 tcgggcgcct gggtgcgcct gacggtgctg gacaacggtc cgggcttccc gcccgagatg
    14701 ctggcgcgcg ccttcgaacc ctacgtcacc accaaggcca agggcaccgg cctggggctg
    14761 gccatcgtca agaagatcgt ggaagagcac aacggcagca tcgagatcgc caatcccaag
    14821 ggcggcggcg cgcaggtgag catcctgctg ccggtcgcga cgcaggcggc gcagcccgag
    14881 cgtgcaccgg tcaacgcgcg agtcggctga gcggccatga gccagatcct ggtagtggat
    14941 gacgagatcg gcatccgcga gctgctctcg gaaatcctcg gcgacgaggg gcaccaggtg
    15001 cggctcgccg agaacgccgg cgaggcgcgc caggcgcgca acagggcgcg gcccgacctg
    15061 gtgctgctcg acatctggat gccggacacc gacggcatct cgctgctcaa ggagtgggcg
    15121 gcgaacggcc agctcaccat gccggtggtg atgatgtcgg gccacggcac catcgacacc
    15181 gcggtcgagg ccacccgcat tggcgcctac gccttcctgg agaagccgat cgcgctgcag
    15241 aagctgctca gcaccgtggg gcaggcgctg cgcgagctgc cgccgcaggc gcagcagcag
    15301 ccgtccctgt cgctcgccta cctcggcaag gccccggtga gcagcgagct caaggagcgc
    15361 ctgacactgc tcaagggact gaagatgccg gtgctgttga ccggcgagcc gggctccggc
    15421 aagagcctgt gcgcgcgcta cctgcagcag gccaacacgc cctgggtggc gcccgacgac
    15481 ttccgcgtgc tggacgaccc ggccaacaat ctggtgggcc aggccgccga gggcacgctg
    15541 ttcctgcacg aggtgagcgc cctcacgctg ccgcagcagc gcaatctgct ggcgcagacg
    15601 gcgaagctcg agcgttatca cacgcgcctg gtctgctcca cctcgcgcgt gctgccgcgg
    15661 ctggtcgccg agggggcctt cgactcgcgc ttgtacgccg gcatcggcca ggtgcaggtg
    15721 cgggtgccga gcctgcgcga gcactcggag gacatcccgg aactggccaa gctgatgctg
    15781 agccgcctgg tggaagcgcg gcacgtgccg ccgcgcacgc tcagcaccgc tgcgctcaac
    15841 gcgttgcgcg tctacgactg gccgggcaac ctcacgcagc tggaaaacgt ggtcaagagc
    15901 atggcgctgc tggcgctgtc cggcgagatc gggctcaccg acgtcaacaa cgcgctgtcg
    15961 cgactggata cgccgccatc cggcgcgctg cccagcctga ccctggaccg ggagctgcgc
    16021 gaggcgcgcg acgccttcga gcgcatgtac ttcgagtatc acatcgcgcg cgaaggcgcg
    16081 aacatgagcc gggtggccga gcgcgtcggc ctcgagcgca cccatctgta ccgcaagctc
    16141 aagcagctcg gcatcaagat cggccggcgc gaatagccgc catccactgc gcgcgcccgc
    16201 gcattcccgg cggcgccggg gcgccgcgct ccgatcactt ataatgagcc ggccgatgcg
    16261 gccgcgctgt gtgcggccgt gcccgaaccc tcctctcgca aaacgtactc gatggcaacc
    16321 cgacaggaac tggccaacgc gctgcgtgcg ctcgccatgg acgcggtgca gaaggccaac
    16381 tccggccatc ccggcatgcc gatgggcatg gccgagatcg ccgaggtgct gtggaatcac
    16441 cacctgcgct tcaacccggc caatccgaac tggccggacc gcgaccgctt cgtgctgtcg
    16501 aacggccacg gctccatgct gctgtacgcg ctgctgcacc tgaccgggta cgacctgccg
    16561 atggacgagc tgcggcgctt ccgtcagctg cactcgaaga cgccgggcca tcccgagcat
    16621 ggcctggcgc ccggcgtgga aaccaccacc ggcccgctgg ggcaggggct cgccaatgcc
    16681 gtcggcatgg cgctcgccga gcgcgcgctg gccgcggagt tcaaccgccc cggctccgag
    16741 atcgtcaacc accacaccta cgtgttcgtc ggcgacggct gcctgatgga ggggatctcg
    16801 cacgaggcct gctcgttcgc gggcaccctg ggcctgggca agctgatcgc catctacgac
    16861 gacaacggca tctccatcga cggccacgtg caggcctggt tcaccgacga cacgcccagg
    16921 cgtttccagg cctacggctg gcacgtgctg ccgaacgtgg atggacacag cttcgaagcc
    16981 gtgcataccg ccatcgagtc ggccaaggcg gccgccgacc gtccgagcct gatttgctgc
    17041 aagaccgtca tcggcaaggg cgcgccgaac aaggccgaca gccacgacgc ccacggttct
    17101 ccgctcggcg agaaggaggt ggcggccacg cgcgagaaca tcggctggcg ccacccgccg
    17161 ttcgagattc ccgccgacgt gtaccagggc tgggacgcgc gcgtgaaggg cgccggcctg
    17221 gaaaagctgt ggcgcaaccg tttcgccgag taccagcgcg cgcacccggc gctcgccgcc
    17281 gaattcgagc ggcgcatggc cggcgacctg cccgccgcat ggcgcgagca cgccgcggcc
    17341 tatctggcgc gcacggtgga gaaggccgag agcatcgcca cgcgcaaggc ttcgcagaac
    17401 gccatcgaag gcctggcgcc caagctgccc gagctgatcg gcgggtccgc cgacctcgcc
    17461 gcctccaacc tcacgatgtg gtccggttcg cgtccggtgc gccgcgccga cggcggcaat
    17521 tatgtcttct acggcgtgcg cgagttcggc atgaccgcga tcatgagcgg gctggcgttg
    17581 cacggcgggt tcatccccta cggcggaacc ttcctgacct tctcggacta ttcgcgcaac
    17641 gcgctgcgca tggcggcgct gatcaaggtg cgcagcatct tcgtgttcac ccacgattcg
    17701 atcggtctgg gcgaagacgg ccccacgcac cagcccgtcg agcacgccgc cacgctgcgc
    17761 tacatcccga acatggaggt ctggcggccc tgcgacagcg tcgagacggc ggtcgcctgg
    17821 accgccgccg tcgagcgcaa ggacggtccc accgcgctgc tgctgtcgcg ccagaacctg
    17881 cccttcgtca agcgcagcgc ggcgcagctt gcgctgatct cacgcggcgg ctacgtgctg
    17941 gccgaggccc agggcgggac gccggccgtg gcgttgctgg ccaccggttc ggaagtcgcg
    18001 ctcgcgctcg aggcccagaa ggcgctgtcg gagcaaggcg tcgccgcgcg cgtggtgtcg
    18061 atgccctgca ccggcatgtt cgaccgccag gacgcggctt atcgccagtc ggtgctgccg
    18121 cagggcttgc ggcgcgtggc gatcgaagcc ggcgtcagcg acttctggcg caagtacgtc
    18181 ggcctcgaag gcggcgtcgt ggggatcgac cgcttcggcg agtcggcgcc tgccggtgag
    18241 ctgttcaagt acttcgcctt cacccccgga cacgtggtgg agaccgtgaa gcgggtcctg
    18301 cacggctgag gcgagcgcga tgggcgacgc cccgttctgg ttgctcgccg acatcggcgg
    18361 cacgaacgtg cgcttcgcgc tgctgccgcc gcgcagcgac cagccgcagc gggagcgcag
    18421 cctgcgctgt gccgactacc ccggtctcga gcaggcggcg cgcgcctatc tggccgacgc
    18481 gggcagtccg cgcgtgaccg aggccgcgat cgacgtggct accgccgtga ccggcgacct
    18541 ggtcaagctc accaacagcc cctgggcgtt ctccatcgag cagacgcgcc ggcagctcgg
    18601 cttggcgcgg ctgctggtgc tcaacgactt cacggcgctg gcgttgtcgc tgcccctgct
    18661 gaacgcaacc gagctgcgac aggtcggtcg cggccaggcg ctcgcgggca aggccatcgg
    18721 gttgattggc gcgggcacgg ggttgggcgt gtcgggactg cttccctgcg gcgcgcgctg
    18781 ggtgccgctc gagggcgaag gcgggcatgt cgcgttcagc ccggtggacg cgcgcgaaga
    18841 cgccgtgctg gcggtgctgc gggagcgctt cgggcacgtc tcagcggagc gcgtgctgag
    18901 cggtccggga gtggtcaacg tctaccaggc gctgtgccgc atcgaccacg tgccgactcg
    18961 tgcgctcgag cccgagcaga tcgcccaggc ggcccggtcc ggggccgacc ctcaatgctt
    19021 ggaagcgctc gccatgttct gcggcgcgct tggtactgcg gcggggaatc tggccatcac
    19081 gctgggcgcc cgcggcggcg tgtacatcgg cggcggcatc gtgcccaagc tcggcgatta
    19141 cttcgagcgc tcgtccttcc gtgcacgctt cgagcacaag ggccggttcg actcttatgt
    19201 cgccggcatc ccgaccttcg tcatccacgc ggactacccg gcgttgcgcg gcctcgccgt
    19261 ggcgctgaat gaggatccta actcctagat ccttttctcg gtctgcggat cgaagaacac
    19321 cagcttggac aggtcgaaca tcagcgtggc gggctggccc gggcgggccc cggcctcggg
    19381 gtgtacgcgg caggtgaccg gcgtgtcgtt gaggtggatc agcaccagcg tgtcgggacc
    19441 ggtgggctgc accacctcga tcttggccac acgcgtcacc acgttcggat tctgtccgct
    19501 gtacggcgtt ttgtcggtga tttgctcggg gcggatgccg agcatcacct cgcggccgtt
    19561 ccaggcgccg agccctttct gcatcggttc gggcgccggg aagaagaagc gctcgctgcc
    19621 ctcgtgcagc tgcacgccga ggcgcccgtc gctttgggtc accgggcagc ggatgaaatt
    19681 catcgacggc gagccgatga agccggccac gaacaggtcg gcggggttgt cgtacacctc
    19741 ctgcggggta gcgagctggc gcaccacccc ttccttcatc acggcgatgc gatcgccgag
    19801 cgtcatcgct tcgatctggt cgtgggtcac gtagacgatg gtggccgcga gccgctcgtg
    19861 cagcagcttg atctcggtgc gcatctccac gcgcagcttg gcgtccaggt tggacagcgg
    19921 ctcgtcgaac aggaacaaag tgggg
//
LOCUS       DASPGE010000095        10934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_380332, whole genome shotgun sequence.
ACCESSION   DASPGE010000095 DASPGE010000000
VERSION     DASPGE010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10934
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380332"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VD791_03035"
     CDS             <1..1029
                     /locus_tag="VD791_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV06975.1"
                     /translation="IPERDTIALFPKEIGGQKVEYIVLDDASDTTTAVKNAKRLVAED
                     RVDLILGPSLTTSSLAMLDIIAEAGTPTVSLASSARIILPMDEKRRWMFKTPQTDVQM
                     ASGIMEHAAARGVKTIAYIGQADTLGEAFYTEVARQAEAHGIKIVASERFNPRDTSVV
                     GQVLRILAAKPAAVVIGSAGTPAALPPKTLVERGYKGVIYHNHGVGNPDFLRVCGKQC
                     DGTYLPASPVLVAAQLPDEHPAKKVALDYIRRYEAAYGPGTVTAFGAYAWDAGLLLQR
                     AVPEALKSAKPGSAEFRKALRDALERVNALAVTNGIINMTPEDHLGLEQRARVMVRIV
                     GGRWVLEK"
     gene            complement(1094..2416)
                     /locus_tag="VD791_03040"
     CDS             complement(1094..2416)
                     /locus_tag="VD791_03040"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HXV06976.1"
                     /translation="MQRSRLVPAVGEAIAKASTAKRCVAVLMLRLKPKDRLFLFTQQD
                     VEAQARKEFEQLAQLLRPADRYCIVDTLQTCILLPDLAAAAQATLAAHKLSRAMAHAS
                     RNPPGQSWARLLIGIACCPDHAEDAESLLMRADAAATQAEQSEDGIWLSDEGSTDGAD
                     GTLPAARAEALEALRSNAFTLAYQPQVDLASGRCESVEALLRAKSTDGGALPPPLLVA
                     AAEEEGKIGFLTSSVLHAALRQLSVWASGGLPMRVSVNLSTHNLRDRGFPDVVARSLA
                     TWGMRAERLTLEIVESSMVHSFTEAATSLHRLKDLGVQLSLDDFGTGYSCLAYLRQLP
                     FDELKVDRAFVHNLNQSAEDRRLVQAIIDLAHNFDLRAVAEGVQDEATLRTLQGMGCD
                     AVQGHVLSEALTADGFTRWLERFANRPAEGDNRPAVDPNLPLTPVRDG"
     gene            complement(2483..4114)
                     /locus_tag="VD791_03045"
     CDS             complement(2483..4114)
                     /locus_tag="VD791_03045"
                     /inference="COORDINATES: protein motif:HMM:NF023299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HXV06977.1"
                     /translation="MPTTIPESRVEKQFSVDELTLGMFVCALDRPWLDTPFLIQGFLI
                     EDEETLAQLRQICGHVTVDLSRSGIDDAEYLGSRPERPARVGANPVPATAPSLLTDQR
                     AQPRSLVPRWLQALFWILGPNWRRPEEKPPLARPKKDLRPAPVDRVKRAAAPKFKAPS
                     GSALERNAAGLGSAAPGDLLAWLGEQLERLRFSGRQGPSDADDPGSANAPRLKRSGSS
                     ARPYYESVPLEQELLRANDRFGAAIDMLGTVMHDIQVGKGLEIEAVESVVEDLVESII
                     RHPGALQLVSRMREADESSYTHALQVGVLLVSFGRELGFSRDELTHLGQVGMLLDIGK
                     LRIDQRILNKRAHLTPKEFDEVKRHVEYGLEIIEASAVAHPDVVTGVAQHHERLNGSG
                     YPQGLQDPEISPIGKMAGIVDTFSALTSIRPHAEPLSPYAAMQQLQGWADTYFNSGLV
                     ELFIQAIGIFPVGTLVELSTGELAVVLEQSRPRRLKPKVLVISAPDKTPLKIPFVLDL
                     LHPECDSADRVPYIRQGLAAASFAVDPREYYVSRS"
     gene            4307..5947
                     /locus_tag="VD791_03050"
     CDS             4307..5947
                     /locus_tag="VD791_03050"
                     /inference="COORDINATES: protein motif:HMM:NF027305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
                     /protein_id="HXV06978.1"
                     /translation="MVVLGWLLGIVIAIVVVAIVVVFLNRFYRKSTRDVALVRTGFGG
                     QRIVISGGCLALPFLHKVDEINMRTIRVEVRRTGDKSLITVDRIRVDVELEFYVRVQP
                     TVEGIATAAQAIGSRSFNPEGIRNLLEGRFVDALQAVAAAETMDSLHERRGEFVRHVA
                     EQLRENLSVNGVLLDSVSLTRLDQASFAALDENNAFNAVGMRKLAEIIASNKKKRTEI
                     EADADIAVRQTQLDATKRRLLLQQEEEQAQIGQRLEVEKVKAASDAEAARAREQAMVA
                     TENARIERERETSALEIGKQRELRKLEIEAQLNSEMRKVDSAIQLAAKHAEEAKAQAA
                     AEMARTEIVLAQEHVQTERERALADRSREMAVKRVQEEGEVAGANAETEASVLIRRAQ
                     ADAQATRSRAEAERARLMAESEGRAALIKAENSRTESLMHLELERYRLDKMPEIVAQM
                     MKPAEKIDSIRIHQVTGFGAATAPNGGVGPEAAARPPVNQVMDSILGMALQLPALKSI
                     GESIGYDFSAALPKPGSSSSGPGFSSSGPGAGGGKPES"
     gene            6042..7268
                     /locus_tag="VD791_03055"
     CDS             6042..7268
                     /locus_tag="VD791_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HXV06979.1"
                     /translation="MGISRREFLAATGAAAVWGGMRPLAAWAQGKDILLGSVLDNSGN
                     LDIYGKPMVMATTMAVEEINAAGGLLGRKIKAIQYDTQSDIALYTKFAQQLARQDKVD
                     VVHGGITSASREAIRQTFKRSNTLYFYNVLYEGGVCDRNCVVTGTTPAQAVEPIVELA
                     MKKWGQKVVILAADYNYGQITAKWLQHYIGQRKGTVASTDFFPLDVSDFGSTIARIQQ
                     AAPNWVVSALVGGAHMAFYRQWAASGMNKKIPLASTTFGVGNEHLALSPAEGDGILIA
                     GNYSQEAKTPANQEFLGRWRKRFGDTKIVHEIAVSQYQGIHLWAECVRKAGTLDRNAM
                     LKTVESGVAIEGPAGTVTIEPKTHHTALDIHVMEVKGQKLTILNTVKQRAPTDTLQYC
                     DLLKNPNENKQYEVKI"
     gene            7420..8289
                     /locus_tag="VD791_03060"
     CDS             7420..8289
                     /locus_tag="VD791_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV06980.1"
                     /translation="MDYTAVVVLEILYMIAFIALISAGLAIVFGMMRVINLAHGEFVM
                     LGGYTTLACERAGIDIWFAMLVIAPLVVALVGLVVERLVIRFLYGRMIDTMLATWGLS
                     LLLIGVVTLIFGSSAVSVPAPIPGYQLGGYQMGGYNLFIIGVTLVVYVATWLVLRYTR
                     VGLVARGAMQSAEVASALGYNPRTVYMITFTVGAALSGLAGGILAPLTGLLPSSGGAY
                     IAEAFIAVITGGAGAVIAGTLASAGIFGAVQQLVSFAHGPVLGDIVMLALAIILLRLM
                     PQGISGRFFRNGQ"
     gene            8286..9335
                     /locus_tag="VD791_03065"
     CDS             8286..9335
                     /locus_tag="VD791_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV06981.1"
                     /translation="MKVWPSIGRIRWGWLLAGAVALLVLPVFTDTHTPTVLLIWALFA
                     LSLGLMWGYAGLLSFGHSAYFGLGAYTYAVAVINIGESTGPLLLAILIPAAVAAVIGA
                     MMFYGRISDVYMGVITLVVTLILFKFMNATAGDQYAIGEARLGGFNGMPGFPVLNVPG
                     LPEVSLFGTPMYYVAAAALLGCYLLSRWILARSFGRVLVGIRENEARCELLGYSVPAY
                     KTAIFSISAAMAGLAGCLFANWAEIVTPGVFALKSAAEVIVWVIVGGLGTLIGPVLGA
                     MALGMVKLLLGEQTMIDNSLILGAILVVVVLAIPRGAVPAVQGWWRGRARIRQQRKVH
                     ARPRRRLQSVQAERV"
     gene            9332..10057
                     /locus_tag="VD791_03070"
     CDS             9332..10057
                     /locus_tag="VD791_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV06982.1"
                     /translation="MSDAVVETIDLTKRFGGVTAVDHVDFALKERELRCLIGPNGAGK
                     STFFKCLTGQYRLDSGNGRVLIRGSDVIGLRPHEIVRMGVGIKTQVPSVMNGLTVMEN
                     LWLAARRAHGWTRSGAEVQRVIEEVGLGELARRTVGELAHGQRQMVEIGIVLAQDPWL
                     VLLDEPAAGITGAEVERLVGLIRQINQNASMIVVDHDMEFVRMLGARITVFHQGAVLT
                     EGPPDKVLSDARVREVYIGNRAR"
     gene            10054..>10934
                     /locus_tag="VD791_03075"
     CDS             10054..>10934
                     /locus_tag="VD791_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV06983.1"
                     /translation="MSSVMDNQVQGAAAEDRPQGVVFEVSDLRAGYGRVPVLHGVSFA
                     LQAGEALGIVGHNGMGKTTLLKALMGLLPVASGRISVDGVDITREPAHARSRLGIAYV
                     PQGRGILPGLTTLDNLRLAWTRDSEESEQEALDRVLTLFPRLSQLLDRRGGALSGGEQ
                     QLLALGRALVASPWMLLLDEPSEGVQPSIVQEIGQMLSAWRAESGLTMIVVEQNLDVV
                     LDVVNRIAVLEKGRIAQMLDTAALGGGQLASVLGLGATRGARGAAAQRIGPAAPAVPG
                     PSSVPGGAPLPGAAAAFP"
BASE COUNT         1754 a         3604 c         3865 g         1711 t
ORIGIN      
        1 atcccggagc gcgacaccat cgccctgttc ccgaaggaaa tcggcgggca gaaggtcgag
       61 tacatcgtgc tggacgatgc ttccgacacg accacggcgg tgaagaacgc caagcggctg
      121 gtcgccgagg accgcgtcga cctgatcctg ggaccctcgc tcaccaccag cagcctggcg
      181 atgctcgaca tcatcgccga ggccggcacg cccaccgtct ccctcgcgtc ctcggcgcgc
      241 atcatcctgc cgatggacga gaaacggcgg tggatgttca agacgccgca gaccgacgtg
      301 cagatggcct cgggcatcat ggagcatgcc gccgcacgcg gcgtgaagac gatcgcttat
      361 atcggccagg cggacacgct gggcgaggcg ttctacaccg aggtggccag gcaggccgag
      421 gcgcacggga tcaagatcgt cgcgagcgag cgcttcaacc cccgcgacac cagcgtggtc
      481 ggccaggtgc tgcgcatcct ggccgcgaag ccggcggcgg tggtgatcgg ttcggccggc
      541 acgccggcgg cgctgccgcc caagacgctg gtggagcgcg gctacaaagg cgtcatctac
      601 cacaatcatg gcgtgggcaa tcccgacttt ctgcgcgtat gcggcaagca atgcgacggc
      661 acctacctgc cggccagtcc ggtgctggtc gccgcgcagc tgcccgacga gcatccggcg
      721 aagaaggtcg cgctcgacta catccggcgc tacgaagcgg cgtacggccc gggcacggtc
      781 accgctttcg gcgcgtacgc ctgggacgcg ggcctgctgc tccagcgcgc cgtgcccgag
      841 gcgctgaaga gcgccaaacc cggcagcgcg gaattccgca aggcgctgcg cgacgcgctg
      901 gaacgggtga acgccctggc ggtgaccaac ggcatcatca acatgacccc ggaggaccac
      961 ctggggctgg agcagcgcgc ccgggtgatg gtgcgcatcg tcgggggcag gtgggtgctg
     1021 gaaaagtgaa tctcgcctcc cgcagcgcct gttctcgcga cagcgacgcc cgcgaagagg
     1081 aaattcaacc gcatcacccg tcgcggacgg gcgttagcgg taggtttggg tcaaccgctg
     1141 gccggttgtc gccttccgct ggccggttgg cgaagcgctc gagccagcgc gtgaatccgt
     1201 cggccgtcag cgcctcgctc agcacatgtc cctggacggc atcgcagccc atcccctgca
     1261 gcgttcgcag cgtggcttcg tcctgtacgc cttcggcgac tgcgcgaagg tcgaaattgt
     1321 gcgcgaggtc tatgatcgcc tgcaccaggc gccgatcttc cgccgactgg ttcaggttgt
     1381 gcacgaacgc ccgatcgacc ttgagctcgt cgaacgggag ctggcgaaga tacgccaggc
     1441 aggaataccc ggttccgaaa tcgtcgagcg aaagctgaac gcccagatcc ttcaggcggt
     1501 gcaggctggt agcggcttcg gtgaagctgt gaaccatcga gctttcgacg atctccaggg
     1561 tcagccgctc ggcacgcatg ccccaggtcg caaggctgcg cgccacgacg tcggggaagc
     1621 cccggtctcg caggttgtgg gtcgacaggt tgacgctcac gcgcatgggc aagccgccgc
     1681 tcgcccacac gctgagctgc cgtagtgccg cgtgcaggac cgagctggtg aggaacccga
     1741 tcttgccttc ctcctccgct gcggcgacca gcaagggcgg cgggagtgcc ccgccgtctg
     1801 tcgacttggc ccgcagcagc gcctcgacgg actcgcaacg gccgctcgcg aggtcgacct
     1861 gcggctggta ggcgagggtg aaggcgttgg atcgcaaggc ttcgagcgct tcggcacgag
     1921 ccgcgggcag cgtaccgtcg gcgccgtcgg tggacccttc gtcggacagc cagataccgt
     1981 cttcgctctg ctcggcctgg gtggccgcgg catccgcgcg catcagcaag gactcggcgt
     2041 cctcggcgtg atccgggcaa caggcgatgc cgatcagcag acgggcccag ctctgcccag
     2101 gcgggttgcg cgatgcatgt gccatggccc gcgaaagctt gtgagccgcg agcgtcgcct
     2161 gtgcggcggc tgcgagatcg ggtaacagga tgcacgtctg cagcgtatcc acgatgcagt
     2221 agcggtctgc cggcctcagc agctgggcga gttgctcgaa ctccttcctc gcttgcgctt
     2281 cgacgtcttg ttgcgtaaag agaaaaagcc ggtccttcgg cttgagccgc agcatcagca
     2341 ccgcgacaca gcgctttgcc gtgctcgcct tggcgatagc ttctccgacg gccgggacga
     2401 ggcgcgagcg ctgcatccgc tgcgcgctcg gttgcttctg cctgtcgggc catctccgtg
     2461 ccgccattct gcccccgtct ggctaggagc gcgacacgta atactccctc ggatccaccg
     2521 cgaacgaggc cgcggccagg ccctggcgga tatatggcac ccggtccgcg gagtcgcatt
     2581 ccgggtgcag cagatccagg acgaacggga tcttcagagg ggtcttgtcc ggcgccgaga
     2641 tcacgagcac cttgggcttg agccgccggg gccggctttg ctccagcacc acggccagtt
     2701 ccccggtgga cagctcgacc agcgtgccca cagggaagat gccgatggcc tggatgaaca
     2761 gctccaccag accggagttg aagtaggtgt cggcccagcc ttgcagctgc tgcatcgctg
     2821 cgtacgggga aaggggctct gcgtgcgggc ggatcgaggt cagagccgag aacgtgtcga
     2881 cgatcccggc catcttgccg atgggagaga tctccggatc ctgcagccct tgcggatacc
     2941 cgcttccgtt gagccgctcg tggtgctgcg caacgcccgt gaccacgtcc gggtgcgcca
     3001 ccgcggaggc ctcgatgatc tccagcccgt attcgacgtg ccgcttgact tcgtcgaact
     3061 ctttcggcgt caggtgtgcg cgcttgttca ggatgcgctg atcgatccgc agtttcccga
     3121 tgtcgagcag catgccgacc tggccgagat gggtcagctc atctcgcgag aagcccagct
     3181 cgcggccgaa gcttaccagc agcacgccga cctgaagggc gtgggtgtag ctcgattcgt
     3241 ccgcctcccg catgcgggat acgagctgga gcgcgccggg gtggcggatg atgctctcca
     3301 cgagatcctc caccacgctc tccacggctt cgatctccag ccctttgccg acctggatgt
     3361 cgtgcatgac cgtgccgagc atgtcgatcg cggcgccgaa acggtcgttc gcacgcagaa
     3421 gctcctgctc gagcggcacg ctttcgtaat agggcctggc gctggagccg ctgcgtttga
     3481 gccggggcgc attggcggat ccggggtcat cggcgtcgct cggtccctgg cggccgctga
     3541 agcgaagccg ctcgagctgc tcacccaacc acgcaagcag gtctcctggc gctgccgaac
     3601 cgaggcccgc cgcgttgcgt tccagtgcgc tccccgaggg cgccttgaac ttgggcgcgg
     3661 cggcgcgttt gacacggtcg accggcgcag gtcgcaagtc ctttttggga cgtgcgagcg
     3721 gcggtttctc ctcaggccgc cgccagttcg gcccgaggat ccagaacagc gcctgcaacc
     3781 accgagggac caggctgcgc ggctgcgcgc gctggtctgt aagcagggac ggagcggtcg
     3841 ctggaaccgg attcgccccg acgcgcgccg ggcgctcggg gcgtgagccc aggtactcgg
     3901 cgtcgtctat gccggatcgg ctgagatcga cggtgacgtg cccgcagatc tgccgcagct
     3961 gcgcgagcgt ctcttcgtcc tcgatcaaga acccctggat gagaaagggc gtgtccagcc
     4021 aaggccggtc cagggcacac acgaacatgc ccagggtgag ctcatcgacc gaaaactgct
     4081 tttccacccg ggactccgga atcgtggtcg gcatggttta gctagtttta tgccaaattc
     4141 ccgcggcgaa gcacgatgtt cgacgcccgt ccagccgtgc gacttgggac cgggggcggg
     4201 tgcccgaagg gaggggctcc tggcccctgc catttcatat acttgacacg gaaaatatcc
     4261 ccgctccata ctccggcagg cttcgtagcg ggaggagggc ggcgccatgg tggtactcgg
     4321 ctggcttctg ggcatcgtca tcgcgatcgt cgtcgtggcg atcgtcgtcg tgtttctcaa
     4381 ccgcttctac cgcaagtcca cccgcgacgt cgccctggtg cgcaccggct tcggcggcca
     4441 gcgcatcgtc atttccggcg gctgcctggc gttgccgttc ctgcacaagg tggacgaaat
     4501 caacatgcgg accatccgcg tggaggtccg ccgtaccggc gacaagtccc tgatcacggt
     4561 cgaccgcatc cgcgtggacg tggagctgga gttctacgtg cgggtgcagc ccacggtgga
     4621 gggcatcgcc accgccgcgc aggcgatcgg ctcgcgctcc ttcaatcccg aggggatccg
     4681 caacctcttg gaaggccgct tcgtggatgc gctgcaggcg gtggccgcgg ccgagaccat
     4741 ggacagtctg cacgagcgcc gcggggagtt cgtgcgccac gtggccgagc agctgcgcga
     4801 gaacctgtcg gtgaacggcg tgctgctcga ttcggtgtcc ctcacccgcc tggaccaggc
     4861 gtcgttcgcg gcgctggacg agaacaacgc gttcaatgcg gtggggatgc gcaagctggc
     4921 cgagatcatc gccagcaaca agaagaagcg caccgagatc gaggccgacg ccgacatcgc
     4981 cgtgcgtcag acccagctcg acgccaccaa gcggcgcctg ctgctgcaac aggaggagga
     5041 gcaggcgcag atcggtcagc gcctggaggt ggagaaggtc aaggcggcga gcgacgccga
     5101 ggcggcacgc gcacgcgagc aggcgatggt cgccaccgag aacgcgcgca tcgagcgcga
     5161 acgcgagacc agcgcgctgg agatcggcaa gcagcgcgag ctgcgcaagc tcgagatcga
     5221 ggcccagctc aactccgaga tgcgcaaggt ggacagcgcg atccaactcg ccgccaagca
     5281 cgccgaagaa gccaaggcgc aggccgccgc cgagatggcg cggaccgaga tcgtgctcgc
     5341 ccaggagcac gtgcagaccg agcgcgagcg cgcgctggcg gatcgctcgc gggaaatggc
     5401 ggtcaagcgg gtgcaggagg aaggcgaggt ggccggggcc aacgccgaga ccgaggcctc
     5461 ggtgctgatt cgccgcgcgc aggccgatgc gcaggccacg cgctcgcgcg cggaggccga
     5521 gcgcgcgcgc ctgatggccg agtccgaggg cagggccgct ctgatcaagg ccgagaactc
     5581 gcgcaccgag agcctgatgc acctggagct ggagcgctat cgcctggaca agatgccgga
     5641 gatcgtcgcg caaatgatga agccggcgga gaagatcgac agcatccgca tccaccaggt
     5701 caccgggttc ggcgcggcca ccgcgccgaa cggcggcgtc ggcccggaag cggcggcgcg
     5761 cccgccggtg aaccaggtga tggacagcat cctcggcatg gcgctgcagc tgccggccct
     5821 gaagagcatc ggcgagtcca tcggctacga tttctccgcg gccctgccca aacccggctc
     5881 cagcagcagc ggacccggct tcagcagcag cggacccggc gccggcggcg gcaagccgga
     5941 gagttgagca cgggtttcct caccggcggg cgggaagcgc gccggtgggg ttttcgaagc
     6001 ggcgcgacgc cgacggcgtt ccaaacactg aggaggaggc gatgggcatc tcgaggcggg
     6061 agtttctggc cgcgaccggc gcggcggcgg tgtggggcgg gatgcggccg ctggccgcct
     6121 gggcccaggg caaggacatc ttgctcggct cggtgctgga caactccggc aacctggaca
     6181 tatacggcaa gccgatggtg atggccacca ccatggcggt ggaggagatc aatgccgccg
     6241 gcgggctgct gggccgcaag atcaaggcga tccagtacga cacgcaatcc gacatcgcgc
     6301 tgtacaccaa gttcgcgcag cagctcgcgc gccaggacaa ggtggacgtg gtccacggcg
     6361 ggatcacttc agcctcgcgc gaagccatcc gccagacctt caagcgcagc aacacgctgt
     6421 acttctacaa cgtgctgtac gagggcggcg tgtgcgaccg caactgcgtg gtcaccggta
     6481 ccacgccggc gcaggcggtc gagccgatcg tcgagctggc gatgaagaag tgggggcaga
     6541 aggtggtgat cctggcggcc gactacaact acggccagat caccgccaag tggctgcagc
     6601 actacatcgg acagcgcaag ggcacggtgg cttccacgga cttcttcccg ctcgacgtgt
     6661 cggatttcgg ctcgaccatc gccaggatcc agcaggcggc gcccaactgg gtggtgtcgg
     6721 cgctggtggg cggtgcgcac atggccttct atcgccagtg ggcggcctcg ggcatgaaca
     6781 agaagatccc gctcgcctcg accaccttcg gcgtgggcaa cgagcacctg gcgctgtccc
     6841 cggcggaggg cgacggcatc ctgatcgcgg gcaactacag ccaggaagcg aagacgccgg
     6901 cgaaccagga gttcctgggg cgctggagga agcgcttcgg cgacaccaag atcgtgcacg
     6961 agatcgccgt gtcccagtac cagggcatcc atctgtgggc ggagtgcgtg cgcaaggccg
     7021 gcacgctgga tcgcaacgcc atgctgaaga cggtcgaatc gggcgtggcc atcgaaggcc
     7081 cggccggcac cgtcaccatc gagccgaaga cgcaccacac cgcgctcgac atccacgtga
     7141 tggaagtgaa gggccagaag ctcaccatcc tgaacacggt caagcagcgc gcgccgacgg
     7201 acacgctgca gtactgcgac ctgctcaaga acccgaatga gaacaagcag tacgaggtga
     7261 agatctaggt ccgagaatcc accccacacc gccccgtcgc ctgcggcgcc accccgcctc
     7321 gtgagaggcg gggagttgtt cttaagtcgc gccttaaggg cgcatccagc ggggtggcgc
     7381 cggaggcgac ggggtggttc gacgctgcat gcgcaacccg tggactacac agcagtagtc
     7441 gtcctcgaaa tcctgtacat gatcgccttc atcgcgctga tcagcgcggg gctggcgatc
     7501 gtgttcggga tgatgcgcgt gatcaacctg gcgcacggcg agttcgtgat gctcggcggc
     7561 tacaccacgc tcgcctgcga gcgtgccggc atcgacattt ggttcgcgat gctggtgatc
     7621 gcgccgctgg tagtggcgct ggtcgggctg gtggtggagc ggctggtgat ccgcttcctg
     7681 tacggccgca tgatcgacac catgctcgcc acctggggcc tgagcctgct gctgatcggg
     7741 gtggtgacgc tgatcttcgg cagctcggcg gtgagcgtgc ccgcgccgat cccgggctac
     7801 cagctcggcg gctaccagat gggcggctac aacctgttca tcatcggcgt cactctggtc
     7861 gtatacgtgg cgacgtggct ggtcctgcgc tacacgcgcg tggggctggt cgcgcgcggc
     7921 gcgatgcaga gcgccgaggt ggcctccgcg ctgggctaca acccgcgcac ggtgtacatg
     7981 atcacgttca ccgtcggcgc ggcgctttcc gggctggccg gcggcatcct ggcgccgctc
     8041 accgggctgc tgccgtcctc gggcggcgcc tacatcgcgg aagccttcat cgcggtgatc
     8101 accggcgggg ccggcgcggt gatcgccggg acgctcgcca gcgccgggat cttcggcgcg
     8161 gtacagcagc tggtgtcctt cgcccacggc ccggtgctgg gcgacatcgt catgctggcg
     8221 ctcgccatca tcctgctgcg cctgatgccg cagggcatca gcgggcgctt cttccggaac
     8281 ggccagtgaa agtctggccc tcgatcggac gcatccgctg gggctggctc ctggccggcg
     8341 ccgtggcgct gctggtgctg ccggtgttca ccgacaccca caccccgacc gtgctgctga
     8401 tctgggccct gttcgcgctc tcgctcggcc tgatgtgggg ctacgccggg ttgctgagct
     8461 tcgggcacag cgcctacttc ggactgggcg cctacaccta cgcggtggcc gtgatcaaca
     8521 tcggcgagag cacaggcccg ctgctgctcg cgatcctgat cccggcggcg gtggccgcgg
     8581 tgatcggcgc gatgatgttc tacgggcgca tcagcgacgt gtacatgggg gtgatcacgc
     8641 tggtggtgac cttgatcctg ttcaagttca tgaacgcgac cgccggcgac cagtacgcca
     8701 tcggcgaggc gcggctcggc ggcttcaacg gcatgcccgg cttccccgtg ctcaacgttc
     8761 ccggcctgcc cgaagtgtcc ctgttcggca cgcccatgta ctacgtggcc gcggcggcgc
     8821 tgctcggctg ctatctcctg agccgctgga tcctggcgcg ctcgttcggg cgcgtgctgg
     8881 tgggtatccg cgagaacgag gcgcgctgcg agttgctggg ctacagcgtg ccggcgtaca
     8941 agaccgccat cttcagcatc agcgcggcga tggccgggct cgccggctgc ctgttcgcca
     9001 actgggccga gatcgtgacg cccggcgtgt tcgccctgaa gtccgccgcc gaggtgatcg
     9061 tctgggtcat cgtcggcggc ctgggaacgc tgatcgggcc ggtgctcggc gccatggcgc
     9121 tgggcatggt gaagctgctg ctgggcgagc agaccatgat cgacaactcg ctgatcctgg
     9181 gcgcgatcct ggtggtggtg gtgctggcga tcccgcgcgg ggcggtgccg gcggtgcagg
     9241 ggtggtggcg cggtcgcgcg cgtatccgcc agcagcgcaa ggtgcacgcg cggccgcggc
     9301 ggcgtctgca gagcgtgcaa gccgaacgcg tatgagcgac gccgtcgtcg agaccatcga
     9361 tctcaccaag cgcttcggcg gcgtgacggc ggtggatcac gtcgacttcg cgctcaagga
     9421 gcgcgagctg cgctgcctga tcggccccaa cggcgccggc aagagcacct tcttcaagtg
     9481 cctgaccggc cagtaccgcc tggacagcgg caacgggcgc gtgctgatcc gcggtagcga
     9541 cgtgatcggc ctgcgcccgc acgagatcgt gcgcatgggc gtgggcatca agacccaggt
     9601 accctcggtg atgaacgggc tcacggtgat ggagaacctg tggctcgcgg cgcggcgcgc
     9661 gcacggctgg acgcgcagcg gcgccgaggt gcagcgtgtg atcgaggaag tgggtctggg
     9721 cgagctggcg cggcgcaccg tgggcgagct ggcgcacggc cagcgccaga tggtggagat
     9781 cggcatcgtg ctggcgcagg acccgtggct ggtgctgctg gacgaaccgg ccgccggcat
     9841 caccggcgcc gaagtcgagc gtctggtcgg gctgatccgg cagatcaacc agaacgcctc
     9901 gatgatcgtg gtggatcacg acatggagtt cgtgcgcatg ctgggcgcac gcatcaccgt
     9961 gttccaccag ggcgcggtgc tcaccgaggg gccgccggac aaggtcctct ccgacgcgcg
    10021 cgtgcgcgag gtctacatcg gcaaccgggc gcgatgagca gcgtgatgga caaccaggtg
    10081 cagggcgccg ccgcggaaga ccgcccccag ggcgtggtgt tcgaggtgag cgatctgcgc
    10141 gccggctacg ggcgcgtgcc ggtgctgcac ggggtgtcgt tcgcgctgca ggccggcgag
    10201 gcgctgggca tcgtcggcca caacggcatg ggcaagacca cgctgctcaa ggcgctgatg
    10261 ggcctgctgc cggtggcttc gggccgcatc tcggtggacg gcgtggacat cacgcgcgag
    10321 ccggcgcacg cgcgcagccg tctgggcatc gcctacgtgc cgcagggccg cggcatcctg
    10381 ccggggctca ccacgctcga caatctgcgg ctcgcctgga cgcgcgacag cgaggagagc
    10441 gagcaggagg cgctcgaccg cgtgctgacc ctgttcccgc gcctgtcgca gctgctcgac
    10501 cgccgcggcg gcgcgctgtc cggcggcgag cagcagcttc tcgcgctcgg ccgtgcgctg
    10561 gtggcgagcc cgtggatgct gctgctggac gagccctcgg agggcgtcca gccctccatc
    10621 gtgcaggaga tcgggcagat gctgtcggca tggcgtgccg agagcggcct caccatgatc
    10681 gtggtggagc agaacctcga tgtcgtactc gacgtcgtca accgcatcgc ggtcctcgag
    10741 aagggccgca tcgcgcagat gctggacacg gccgcgctcg gcggcgggca actcgcctcg
    10801 gtgctcgggc tgggcgccac gcgcggcgcg cgcggcgcag cggcgcagcg catcggtccg
    10861 gccgcgcctg ctgtgcccgg cccgtcgtcc gtgcccgggg gggcgcccct accaggcgcg
    10921 gcggccgcgt tccc
//
LOCUS       DASPGE010000096         4130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_498154, whole genome shotgun sequence.
ACCESSION   DASPGE010000096 DASPGE010000000
VERSION     DASPGE010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498154"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /locus_tag="VD791_03080"
     CDS             <1..1590
                     /locus_tag="VD791_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HXV06984.1"
                     /translation="LPPLPLPAAPEADEVPEEDEVPEADEAWQDAWQAIAPQQAPSAE
                     EAEEEDIDGLPDGLSWADDEPPPKLPTRPRGTLAPPGERSPPASLSAEQKLLLLDTWQ
                     RSGLPASDFAALVHVTRQTLCAWKRRFEELGPAGLVDRPKGAKPGSKLPELTRRTILM
                     LKQTHPDWGCQRISDMLVRGPALPASPNAVARVLREAGYELVEQPTRPHPDHVRRFER
                     ARPNQLWQTDLFTFVLKRQNRRVYLVAFLDDHSRFITGYGLHGSQSTPLVIEVLRAAI
                     TAYGVPAEILTDNGAQYVTWRGKSQFTKELEKLGVRQIVARPKRPQTLGKVERFWGTL
                     WRECVESAVFLDLEDARRRIGLFIDHYNFQRVHSGIDSLVPADRFFQAAPQVLATLKE
                     RVAASALKLARHGVPKQPFYLTGQVEGRPFSVHQAGDRVILQQAGAEHTEIELAAPLG
                     TAALGTTTGGAASAASASQGAAQQPPALPAAICPDGSPPQSGPLPEELPPGVSALDDY
                     LAQRGAPQPGMEAGEDAGGDA"
     gene            1587..2225
                     /locus_tag="VD791_03085"
     CDS             1587..2225
                     /locus_tag="VD791_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06985.1"
                     /translation="MNKKKSSPSSRKQAAAKEASPPAATPSDAQRLAAAILEVLAGLR
                     SPPQAAELLGVSLPRYYQLEARALEGLVAALAPRPKGKQPALENRVRQLEQELEAARR
                     ACARQEALVRVTQRSLGLAALAKPPAKANPKANANGKADSQADAPAAGRRRKPRRPLV
                     RALRAAQQLRTQADRAEQPQASDQTQDAGAGNSAPPAVQPIAPNGAGEETRP"
     gene            2262..2765
                     /locus_tag="VD791_03090"
     CDS             2262..2765
                     /locus_tag="VD791_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXV06986.1"
                     /translation="MAGRKPLGPALVEHLEGSASAKERLEMILATIAGQVSVVDAAEL
                     LGISEAMFYRLRSRALQVSLEDLEPKPIGRPPGTSATSPEQLHSAALQSRVAELEREL
                     AAHTVRLELAQTIPHVLQPPAMQLHTTSPQIDAPASPAAVKKTRRLARQRRQLRQRQR
                     QARRKAK"
     gene            2780..4009
                     /locus_tag="VD791_03095"
     CDS             2780..4009
                     /locus_tag="VD791_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HXV06987.1"
                     /translation="MEEEVTPSPATSDDAVTGDAVPVEVAPVEVVPVEVVSPSRRERH
                     AARQRSRRTQEHQTRTSAVQFAAEHARSCRQAARRLDLSPRTLAAWKRRQALGELAPH
                     PRGRPCREPTHDQRAVVVAILEETGPRLGLPTLRSCTPDTPPCVLAHLLDEHRREFQA
                     EHRQQAETLHWQQAGAVWAIDHSEPPRPVDGCYTQILALRDLASGLQLAWTPVPDATA
                     AEALIVLEGLVRAHGSPLVLKSDNGSAFKSSDFAAWLEGWQIVPLFSPVRMPRFNGAC
                     EAGIGAAKRRTEYLAARHGRPLDWTSDDLYAAQLWANDDHYPHGLAAGAPADRFAARP
                     PIAEIARDNFHAAVVQYEDQLNREFCTASLALTDTLRAMHHRRAVRRALVELGYLDIT
                     RRSIPQPLPPAKRAGIT"
BASE COUNT          693 a         1498 c         1432 g          507 t
ORIGIN      
        1 ctcccaccgc tgccgctccc agcggccccc gaggccgacg aagtccccga ggaagacgaa
       61 gtccccgaag ccgacgaggc gtggcaggac gcctggcagg cgatcgctcc acagcaggcg
      121 ccatctgccg aagaggccga agaagaagac atcgacggcc tgcccgatgg cctgtcgtgg
      181 gccgatgacg aaccgccgcc gaagctcccc acgcgccccc gcggcacgct cgctccgccc
      241 ggcgaacgat cgccgcccgc gtcgctgtca gccgagcaga agctgctgct gctggacacc
      301 tggcagcgga gcggcctgcc ggccagcgac ttcgcggcgc tggtccacgt cacgcggcag
      361 acgctctgcg cctggaagcg gcggttcgaa gagctcggcc cggccggact ggtcgaccgc
      421 cccaaggggg ccaagccggg aagcaagctg ccggagctca cccggcggac gatcctgatg
      481 ctcaagcaga cgcatcccga ctggggctgc cagcggatca gcgacatgct ggtccgcggc
      541 cccgcgctgc cggccagtcc gaatgccgtg gcgcgggtgc tgcgggaggc gggctacgaa
      601 ctggtcgagc agcccacgcg tccgcatccc gatcacgtcc gccgcttcga gcgggcccgg
      661 cccaaccagc tctggcagac cgacctgttc acgttcgtgc tcaagcggca gaaccggcgg
      721 gtgtacctgg tcgccttcct ggacgaccac agccgcttca tcaccggcta cggcctgcac
      781 ggcagccagt cgacgccgct ggtgatcgag gtgctccggg cggcgatcac ggcctacggc
      841 gtgccggcgg agatcctcac cgacaacggc gcgcagtacg tcacctggcg cggcaagagc
      901 cagttcacca aggagctgga aaaactgggc gtgcggcaga tcgtggcccg gcccaagcgg
      961 ccgcagacgt tgggcaaggt cgagcgcttc tggggcacgc tgtggcgcga atgcgtcgag
     1021 agcgccgtgt tcctcgacct ggaagacgcc cggcggcgga tcgggctgtt catcgaccac
     1081 tacaacttcc agcgcgtgca ctcgggcatc gacagcctgg tgccggccga ccgcttcttc
     1141 caggcggcgc cgcaggtgct ggcgacgctc aaggagcggg tggcggcaag cgcgctcaag
     1201 cttgcgcggc atggcgtgcc gaagcagccg ttctacttga cgggccaggt cgaaggccgg
     1261 ccgttcagcg tgcaccaggc gggcgaccgc gtgatcctgc agcaggccgg cgcggagcac
     1321 acggagattg agctggcagc gccgctgggg acagcggcgc tgggaacgac aaccggcggc
     1381 gcggcatccg ctgcttctgc gtcccagggc gcagcgcagc agccgccagc gctgccggct
     1441 gcgatctgtc ccgatggatc gccgccgcaa tccggcccgc tgccggaaga actgccgccg
     1501 ggcgtctcgg cgctcgacga ctatctcgcg cagcgcggtg cgccgcagcc gggaatggaa
     1561 gcgggagagg atgcgggagg tgacgcatga acaagaaaaa gtcctcgcct tcgtcacgga
     1621 agcaagcggc cgcgaaggag gcttcgccgc cggccgcgac gcccagcgac gcgcagcgcc
     1681 tggcggcggc gatcctcgaa gtgctggccg gcctgcgctc gccgccgcaa gcggccgaac
     1741 tgctgggcgt ctcgctgccc cgttattacc agctcgaagc gcgggccttg gagggcctgg
     1801 tggcggcgct ggcgccgcgt cccaagggga aacagccggc gctggaaaac cgcgtccggc
     1861 aactggagca agagctggaa gcggcccggc gggcctgcgc ccggcaggag gcgctggtcc
     1921 gcgtcacgca gcggagcctg gggctggccg cgctcgccaa acctccggcc aaggcgaatc
     1981 ccaaggccaa cgccaacggc aaggccgact cccaagccga tgctcccgca gccggcaggc
     2041 ggcgcaagcc gcggcggccg ctggtccgcg cgctgcgggc cgcgcagcag cttcgcacgc
     2101 aagccgaccg tgccgagcag ccgcaggcgt ctgaccaaac gcaagacgcc ggcgctggca
     2161 attccgcacc gcccgcggta caaccgatcg cgccaaatgg cgcgggagag gagacccgcc
     2221 catgatcggc gacagacccc aggaggtcgg agacttcccg catggcagga cgcaaaccgt
     2281 tgggaccggc gctggtcgag cacctggaag gctccgccag cgcgaaagaa cgtttggaaa
     2341 tgatcctggc cacgatcgcc ggccaggtgt cggtcgtcga cgccgccgag ttgctcggca
     2401 tcagcgaggc gatgttctac cggctccgca gccgggcgct gcaggtcagc ctggaagacc
     2461 tggagcccaa gccgatcggc cggccgcccg gcaccagtgc gaccagcccc gagcagttgc
     2521 acagcgcagc gctgcagtcg cgggtggcgg agctcgagcg ggaattggcg gcgcacacgg
     2581 tgcggttgga gttggcccag acgattccgc acgtcctcca gccgccggcg atgcaactgc
     2641 acacgacttc gccgcagatc gacgcgccgg cctcgcctgc ggcggtaaaa aaaacgcgcc
     2701 gcctggcccg gcagcggcgg cagctccgcc agcggcagcg ccaagcgcgg cggaaggcga
     2761 agtagcgccg ctctgcgcgg tggaagagga agtcacgccg tcgcccgcga cgtccgacga
     2821 cgcagtgaca ggcgacgctg tgccggtcga agtcgcgccg gtcgaagtcg tgccggtcga
     2881 agtcgtgtcc ccttcgcgcc gcgagcggca cgccgcgcgg cagcgatcgc ggcgcacgca
     2941 ggaacaccag acacgaacct cggccgtgca gttcgcggcc gagcacgccc gttcctgccg
     3001 gcaggcggcg cggcgattgg atctctcgcc gcgcacgctg gccgcctgga aacggcggca
     3061 ggcgctggga gaacttgcgc cgcacccgcg cggccggccc tgccgcgaac cgacgcacga
     3121 ccagcgggcc gtcgtcgtgg cgatcctcga ggagacgggg ccgcggctcg gcctgccgac
     3181 gctgcggtcc tgtactcccg acacgccgcc ctgcgtcctt gcgcacctgc tcgacgagca
     3241 ccgccgcgag ttccaggccg agcaccgcca gcaggcggag acgctgcact ggcagcaggc
     3301 cggcgcggtc tgggcgatcg atcacagcga gccgccgcgg ccggtcgacg gctgctacac
     3361 ccagatcctc gccctgcgcg acctggccag cggcctgcag ctcgcctgga cgccggttcc
     3421 cgacgcgacc gccgcagagg ccctgatcgt gctcgagggg ttggtccggg cgcacggttc
     3481 gccgctggtg ctcaagagcg acaacggctc ggcattcaaa agcagcgact tcgccgcctg
     3541 gctggagggc tggcagatcg tgccgctctt ctcgccggtc cgcatgccgc gcttcaacgg
     3601 cgcgtgcgag gcgggcatcg gcgccgcgaa gcgccgcacc gagtacctcg ctgcgcggca
     3661 cggccgcccg ctcgactgga ccagcgacga cctttacgcc gcgcagctct gggccaacga
     3721 cgaccactac ccgcacggtc tcgccgccgg cgcccccgcc gaccgcttcg ccgcgcggcc
     3781 gccgatcgcc gaaatcgcgc gtgacaactt ccacgccgca gtcgtacaat acgaagatca
     3841 actcaaccgc gagttctgca ccgcaagcct cgcgctgacc gatacgttac gagccatgca
     3901 ccaccgtcga gccgtccgcc gtgctctcgt cgagctcggc tacctcgaca tcacccggag
     3961 gtcgattcct caaccccttc cccccgccaa acgcgcagga attacgtgag ggacacagtg
     4021 tctacagggt gggcgagagc ggagtgctgg tgcataacaa ttcagcaagt attgtccctg
     4081 atgccaacaa ctccccaaca agccacaagg cgaccgactg tacggactcg
//
LOCUS       DASPGE010000097         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_264537, whole genome shotgun sequence.
ACCESSION   DASPGE010000097 DASPGE010000000
VERSION     DASPGE010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264537"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..912)
                     /locus_tag="VD791_03100"
     CDS             complement(139..912)
                     /locus_tag="VD791_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HXV06988.1"
                     /translation="MDLSEKLLRLKPEAMSGEEWQARLELAALYRAFDWLGWTESIFN
                     HITARVPGGERHYLINPYGLNYSEVTATNLVKVNLAGETMDGSRYPVNKAGFIIHSAV
                     HAAREDAHCVIHTHSTAGSAVACKKDGLRFDNFYSALLYGQVAYHDFEGVTTDAAEQP
                     RLVKSLGDKAILILRNHGLLVACPSVPQAYLTYWGLQRACEIQVATDAMRGDNLPIAP
                     RVLEQTPARSQAFNSDPRPGGLPFDALLRRAGIHYEDIV"
     gene            complement(933..2072)
                     /locus_tag="VD791_03105"
     CDS             complement(933..2072)
                     /locus_tag="VD791_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV06989.1"
                     /translation="MTLGGAGMYAVTVALPPVQAEFGVGRSEASLPYTMTMIGFGLGG
                     IVMGRLADRFGVMVPVLIGALCLGAGFITAGLAGSLWQFALAQGVLIGFLGCSATFAP
                     LVADTSLWFNRRRGIAVAICASGNYLGGTVWPPVVQHFFDTAGWRATYIGVGAFCIAA
                     MLPLALVLRPRPPLPVLAPGVKAGQAPHDLGLRPALLLALLCTAGVACCVAMSMPQVH
                     IVALCSDLGFAAARGAEMLSLMLGFGIVSRLAFGWISDRIGGLRTLLLGSALQGVALL
                     LFLPADGLVSLYVVSALFGLFQGGIVPSYAIIVREYFDARTAGATVSIVLTATLVGMA
                     LGGWMSGALFDLTGSYRAAFLNGLAWNLLNMTIAGALLLRARKRL"
BASE COUNT          357 a          734 c          760 g          302 t
ORIGIN      
        1 ccacgaacgg ctggttgaag tcgtcgaccg aggccatggc cttgtccacg tagccggcgc
       61 ccagcacctc gcgccgcgtc ttcagcccgt cgtcgaaccg ctccttctgg ctcctcttgt
      121 ccatcctcgg atctccattc agacgatgtc ttcgtagtgg atgcccgccc gccgcagcag
      181 ggcgtcgaac ggcaggccgc cgggtcgcgg gtcgctgttg aacgcctgcg agcgcgccgg
      241 cgtctgctcg aggacgcgcg gcgcgatggg cagattgtcg ccgcgcatgg cgtcggtcgc
      301 cacctggatt tcgcaggcgc gttgcagccc ccagtaggtc aggtaggcct gcgggacgct
      361 cgggcaggcc accagcagcc cgtggttgcg caggatgaga atggccttgt cgccgaggct
      421 cttcaccagc cgcggctgct cggcggcgtc ggtcgtcacg ccctcgaagt cgtggtaggc
      481 gacctggccg tagagcagcg cgctgtagaa attgtcgaag cgcaggccgt ccttcttgca
      541 ggccacggcg ctgccggccg tcgaatgcgt atggatgacg caatgcgcgt cctcgcgcgc
      601 cgcgtggacg gcgctgtgga tgatgaaccc ggccttgttc accggatacc tcgagccgtc
      661 catggtctcg ccggccaggt tcaccttgac gagattggtg gcggtcacct cgctgtaatt
      721 gaggccgtag ggattgatca ggtagtggcg ctcgccgccc ggcacgcggg cggtgatgtg
      781 gttgaagatc gactcggtcc agcccagcca gtcgaaggcg cggtacagcg ccgcgagctc
      841 caggcgggct tgccactcct cgccgctcat ggcctccggc ttcaggcgca gcagtttttc
      901 gctcaggtcc atgcgtgcct cctcggcgcc gactatagcc gctttcgggc gcgcagcaag
      961 agcgcgccgg cgatcgtcat gttgagcaga ttccaggcga ggccgttgag gaacgcagcg
     1021 cggtaggacc cggtgaggtc gaacagcgct cccgacatcc agccgccgag cgccatgccc
     1081 accagcgtgg cggtgagtac gatgctgact gtggcgcccg cggtgcgggc atcgaaatac
     1141 tcgcgcacga tgatcgcata ggacggcacg atcccgccct ggaacaggcc gaacagcgcg
     1201 gacacgacgt agagcgatac gaggccgtcg gccggcagaa agagcagcag cgccacccct
     1261 tgcagcgcgg atcccagcaa cagcgtgcgc agcccgccga tgcggtcgga gatccagccg
     1321 aaggcgagcc ggctgacgat gccgaagccc agcatgagcg agagcatctc ggcgccgcgc
     1381 gccgcggcga agccgaggtc gctgcacagc gcgacgatgt gcacttgcgg catggacatc
     1441 gccacgcagc aggccacccc ggcggtgcag agcagggcga gcagcagggc gggccgcagg
     1501 cccaggtcgt gcggcgcctg gcccgccttg acgcccggcg cgagcaccgg gagcggcggg
     1561 cgcgggcgca gcaccagggc gagcggcagc atggccgcga tgcagaacgc ccctacgccg
     1621 atgtacgtgg cgcgccatcc ggcggtgtcg aagaaatgct gcactaccgg gggccagaca
     1681 gtaccgccca ggtagttgcc gctcgcgcag atggccaccg cgatgccgcg gcggcggttg
     1741 aaccacagcg aagtgtcggc caccagcggt gcgaaggtcg ccgagcagcc gagaaacccg
     1801 atcagcacgc cctgcgccag ggcgaactgc cacaggctgc cggcgagccc cgcggtgatg
     1861 aagcccgcgc ccaggcacag cgcgccgatc aatacgggga ccatgacgcc gaagcggtcc
     1921 gcgagccggc ccatgacgat gccgcccagg ccgaagccga tcatggtcat cgtgtagggg
     1981 agcgaagctt ccgagcggcc gacgccgaac tccgcctgca ccggcggcag ggcgaccgtg
     2041 accgcgtaca tgcccgcgcc gccgagcgtc atcagcgcga gcgtcgccca caggcgcagc
     2101 caggtgtagc gggattccgc ctcgtgttgc acgatcgtcg gctacgcggc ggg
//
LOCUS       DASPGE010000098        11580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1551733, whole genome shotgun sequence.
ACCESSION   DASPGE010000098 DASPGE010000000
VERSION     DASPGE010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11580
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551733"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2623)
                     /locus_tag="VD791_03110"
     CDS             complement(<1..2623)
                     /locus_tag="VD791_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06990.1"
                     /translation="MWKNLTRRARITVLLVIHAALPLSLLGILTASQSGEAAQASAAP
                     GFSIKPARPWTASGVRYRNAQEYVVTGKALGRKEKAAAAAVLAHMLVTTEPRTSHEDA
                     LKRLADIAASRDATIEYVEIGGWPAVELQFTEALRRRGQAADGSEPVQVPRAITAIAA
                     GNKVLHFDVSLAPEAPLDLLDTAKGVARTATFPKRGNPEEVKRALETLPMEVTRRRSL
                     RQPSEEPVTTPPSSEGAESTRPNQALEVAQGTRRPSDLAPSMAAAAGQAAPGAGNVGA
                     VRVQNGVGELEIAASANAMNVVIASNAALSFSTNRGSTYAAGATGVFGLNDPTLARAV
                     SGNFYLGVIAFPNGTAAHLNVTGCTNAVSRSTDNGANFALQGYSARCPQSGTGLCFPD
                     QEHIAADTVTASGGNDQLYAVWRNFTPAGTPPATCAGIGSGFVAPSISCSQNNGTTWT
                     ATAVIPGAGDFPRVAVGRDGSVFVVTLSGNSVLLNRFASCANGLTAAAGFPVTVATLS
                     GAVVCPVSGLDRCNDGNTLSSPTVAPDPANANHLFVSFAERDGAGGERIVTRESTDRG
                     ATFPTARTVSANQSTRRFMPWVCSARGAAWTGWYDRRASGAAGASNDLTDYFLGSTLG
                     HEWNLSGNADPQCASGWPCAPRSTNDSESCTIQPQLAGVCRAAGGGGSGNRCDFSSPA
                     CPAGETCMGGGGCPKYGDYNGVACSGNFVFAAWASATTPPGVPAGTGLRVFSSTLFVA
                     RNGASIWRHTGTPCAGDSCPGWQRLDNNHKTVSIVSAGSRIYQLHNDGWIWQGTGTAC
                     SSDSCPGWIRLDNNSKTIAIAAADGQLYQLHNDGWIWRYTGTPCSGDSCPGWQRLDNN
                     PKTTAIAAAGSQLYQLHN"
     gene            2853..3929
                     /locus_tag="VD791_03115"
     CDS             2853..3929
                     /locus_tag="VD791_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06991.1"
                     /translation="MDILSQLAISALDDALSGFLPSTVPSGLTRRVRVVPSEVRASGL
                     GGYVGTHPEPRGAVLARRVRATLELAVTGGNDADASGYLNQVVRTLLSQQQGDLRREG
                     IQRITLNAAAVQPRSATLELDYEYRHLPTESEGVIELLDLNLDVNLTPYRVKYRWDLA
                     TRTLVGASQPLADFQVADDPDLNSGSPASQWTFNEAQARIEQNALTRGGPLALSQPKK
                     AGAQLLWRPGGEAFELGRFIAAVDFESGSSDGIGVVFGRKDAQNFWYFLASERHRYHL
                     FGRKDAGAYSFVGTPAGDVGFALNSRHVLVLVVYDQTLIAELDGVRTLKAEADVQVDA
                     GEIGFLTHGNNQARFHRARLIELV"
     gene            4002..5033
                     /locus_tag="VD791_03120"
     CDS             4002..5033
                     /locus_tag="VD791_03120"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HXV06992.1"
                     /translation="MQAEALIAPKGVAFGNVGIVGTAANAQGASALGTTQIISSLEDA
                     TRLYAPSDALSAGTLNLMRGMELLFRNGANTVYARGLAQGATQQDFANAVDELLKDEI
                     NLLVAPELTTSVATTVLKAAVNSAETNARDVIAVIGADATTVANIEDQVQADKRVVLC
                     TPGVFAFDAAAGAEVSLPGNYTAAAVAGLIASLAPQSSPTNKVLPGITKLGIKFGYGE
                     LKALLQANVLPLESRQGIRVVRGITSEGEAFAQITTRRIVDFAKAGIRQVANPFIGRL
                     NNERVRKALQGALDGFLTTMLVDEALTHYTVEVKATRADEIAGRCVVNVVMQPTFSID
                     YIRVTLALS"
     gene            5052..5555
                     /locus_tag="VD791_03125"
     CDS             5052..5555
                     /locus_tag="VD791_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06993.1"
                     /translation="MANTSVFTGADGSITLSTTSSAGREAEVAQGIIDGNDLITVGRA
                     TGVAVEVTSEIHAFHEIGQRYATELRPGNVAVHGTIKRAYLNGAMLRLLLGEAADGRP
                     SNSWAQPSFNITLLAENAAVPGVRSTITLHGVKLDQWSYNVPEDDFIVESASFQALFL
                     TVADEGA"
     gene            5653..6120
                     /locus_tag="VD791_03130"
     CDS             5653..6120
                     /locus_tag="VD791_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06994.1"
                     /translation="MLTADELLAGANLTFDIELPPHVLGGSGPAPAGAVRLRPLTVRD
                     LQLIARAAKDNDALAGALMVQAALVEPKLTLPQVNALQVGLLQFLLTEVNRVSGIGAD
                     AVSVRGAAEDPLIRAAFLLAREFGWTPEQVGELTLGQILLHLQLLKEQRVANA"
     gene            6209..7141
                     /locus_tag="VD791_03135"
     CDS             6209..7141
                     /locus_tag="VD791_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06995.1"
                     /translation="MRQLDDKATLWSCVRALDEVPACITFHPFTATADWSVDLQREQA
                     HAVRAEQLQSWSPELMRRARRRQQISVVGGAAALSPSAASPPRALKADSTLADARLPG
                     ARSAAQVPRGLMPEAGVWNALLAIVQLIDEIETAPEPGDVPTAVPGASVSSQPRSDNA
                     DTADIPPEPKATAVAATALLSPPGKIAAANSTSAAGSEPQPATALLGQYVAALWSGAA
                     APLSSRSTAAHTASRAAVEPASAARPRAPSLLLPARAPRETPPGPEPEARSADGSSPA
                     TAWPANDPGHEDALAERLNRALLEQAWLRGVDLT"
     gene            7161..7751
                     /locus_tag="VD791_03140"
     CDS             7161..7751
                     /locus_tag="VD791_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06996.1"
                     /translation="MSLVPMLGDWEVPSVTRLETLESRALAEMEIPGRAGNLFQDLNR
                     KPARVVVEGSVFGQDAGQEFLTSVREKFVAGEPLTFVSDIATGTEIQHVLVQQLHVQA
                     AASHADQIDYTLWLVECPPPPPPSDILGGIDTGLLDQAAGMLDTALGAIDALEALGSV
                     PNLSDPTKPLAGALDDVKGAVDQLGEVGASLQGLFG"
     gene            7755..11207
                     /locus_tag="VD791_03145"
     CDS             7755..11207
                     /locus_tag="VD791_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06997.1"
                     /translation="MPTLLEQLQSVTGGSNVTLDVAAQAGQFTQIAQLVQQLASEPPG
                     DFGGYLSQLQGLALPNVSLGGQLGQSFGQLLPDLQGGLGGMLQPLLDSAGNIQQGVGA
                     DLAGALGPLLEAIAKLRDLVASDWTCGLVPALAPPPAPEPPPGPPPPEPPPPGPPPAP
                     STPAPVLTAAQVTQAKAVLDTLPGDMSVEALLKWLHQRVGTFRPGRYLYLRSIPVLDD
                     LRDPLDTLVRWDGFTGAQLAQELAQTLNAMAAIITANTTDIAGGPFFAGTVQAIPAQG
                     FATAGDALGDALDALAAAVQAQNAATIATQLAAAQSAATQVEAANVTLDAQQAAVEQM
                     EEMARALPSALETTMCRLLVLSQPRATWGDLSKRFGTPTVLPPDAFAPLTDLFDRLQE
                     FLDGVLDAVDIGAVTQPIAQALTQVTQAIQQLEQGLAQLTASARAAFEQAHTALQALD
                     LEAVLQQADQSLQAALQQVQQAIAQGLAPAATALSDALTAVDGALAGFDPEQLAQPVQ
                     QVMDAIKSVFEDAAVQQAIQQLERLKEMADKLDELSFRPVSDVVIGGIGTVKSALEAI
                     DEASLPAPGPELISEAMSVLPESLTPLTDPLTSNLDELIEKGPIPLLEALRDLPKPIV
                     EEMRNFSPRGLLEEPLGTPFRELRGQLDEFQPTQWLDAAEQELDSLKQRLLQALDLEA
                     LLAPLAQAQQALLQELGRFRPGAVIEPLAQQLEQALESLDVALPTTQITQELDQVLGR
                     VQSILGTFDQVVEVVQAFTTKLALLADPDAQLDQWLTEILAKLPADAPATLSAPLAAL
                     AQAVDNARATPLHTAYQSARQPLADKLALADPAALLVRILQGRSRLTPALVNALPASQ
                     AKTDLQQFLTQFDPAGPGFSRGLRRLAGLRDALSAADQQMTTLFAEWDARYHRSDGVL
                     AALVHQSVTVDELRGWLREAVDKQLGAPVVGFLKQLKLVGTLLGTFGDAIAGVVQAVK
                     DKIDDLLAVPQALSQAAHELEQLQQQITSLDLDMFVREVDSLYEELLDTLRALDPGAM
                     EQPLRRALEALLDDLTLDSVFTPGLRDQIEQSYATLVGKVDALDPELLVIKPLDDLYT
                     QDVLPLVDALDVSEAVQKLIDRLNALPDELKEELGRVDEAYQDMLAAAPSGAPSSGSA
                     SVSL"
     gene            11221..>11580
                     /locus_tag="VD791_03150"
     CDS             11221..>11580
                     /locus_tag="VD791_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06998.1"
                     /translation="MALGSLLLQPKTLERMQTLDANQALADDIVALLHQILVNPDHLD
                     SVAPAFRKAFGGLGERMRGRLESRLQSALDQLRDLLGPVLQAGQDVAANGQHLDSAGE
                     VFELIATALDKLIAALQA"
BASE COUNT         1897 a         3960 c         3916 g         1807 t
ORIGIN      
        1 cgttgtgcag ctgatagagc tggctgccgg ccgcggcgat tgccgtcgtc ttcgggttgt
       61 tgtccaacct ctgccagccc gggcagctgt cgccgctgca gggcgtaccg gtgtagcgcc
      121 agatccagcc gtcgttgtgc aactgataga gctgaccgtc cgccgcggcg atcgcgatgg
      181 tcttggaatt gttgtcgagt cggatccacc ccggacagga atcgctgctg caggcggtgc
      241 cggtcccctg ccagatccag ccgtcgttgt gcagctgata aatgcgactc cccgcactga
      301 cgatcgagac cgtcttgtgg ttgttatcca ggcgctgcca gcccgggcac gaatcgccag
      361 cgcaaggcgt gccggtgtgg cgccagatcg acgcgccatt gcgggcgacg aacagcgtcg
      421 aggaaaacac ccgtagtccg gtgccggcgg gcacacccgg cggtgtcgtc gccgatgccc
      481 acgccgcgaa cacgaaattg cccgagcacg cgacaccgtt gtagtcgccg tacttcgggc
      541 agcccccacc gcccatgcac gtctcgccgg ccggacacgc tggactgctg aaatcgcagc
      601 ggttgccgct tcctccgccg ccagccgcgc gacatacccc tgcgagttgc ggttggatgg
      661 tgcaggactc cgagtcgttc gtactgcgcg gcgcgcaagg ccagccggag gcacactgcg
      721 ggtcggcgtt gccggagaga ttccattcgt gccccagtgt cgatcccaga aagtagtccg
      781 tcaggtcatt gctcgcaccc gcggctccgc tggcgcggcg gtcgtaccag ccggtccatg
      841 cagcaccgcg tgccgagcac acccagggca tgaagcgccg cgtcgactga ttggcgctca
      901 cggtccttgc ggtggggaaa gtggccccgc gatcagtcga ctcgcgcgtg acgatacgct
      961 cgccgcccgc accgtcgcgc tcggcaaagg acacgaagag atgattggcg ttggccggat
     1021 ccggcgcgac ggtggggctg ctgagcgtgt tcccatcgtt gcagcgatcg aggccagaaa
     1081 ccgggcagac caccgcgcca ctcagcgtgg ccaccgtgac ggggaatccc gccgccgcgg
     1141 tcaacccgtt cgcgcacgag gcgaagcggt tgagcaggac actgttgccg ctcaacgtta
     1201 cgacaaacac gctgccgtca cgaccgaccg cgacgcgcgg aaaatcgccc gcaccgggga
     1261 tcacggcagt ggccgtccag gtcgtaccgt tgttctgcga gcagctgatc gaaggcgcaa
     1321 cgaagcccga cccgatcccg gcgcaagttg caggcggggt gccggcgggc gtgaagttgc
     1381 gccagaccgc gtacagctga tcgttgccgc ccgaggccgt taccgtgtcg gccgcgatgt
     1441 gttcctgatc ggggaaacac agcccggtgc cgctctgcgg acagcgcgcg ctgtagcctt
     1501 gcaacgcaaa gttcgcgccg ttgtccgtcg agcggctcac cgcgttcgta caaccggtga
     1561 cattgaggtg tgcggctgtg ccgttcggaa acgcaatcac gccgagatag aaattgccgc
     1621 tgacggcgcg cgccagcgtt ggatcgttca ggccgaacac gccggtcgct ccggccgcgt
     1681 aggtggatcc gcgattggtc gaaaacgaca acgcggcgtt gctcgcgatc acgacgttca
     1741 tcgcgttcgc actcgcggcg atttccagtt cgcccacgcc gttttgcact cggactgccc
     1801 caacgttgcc ggcgccaggc gcagcctggc ctgccgcggc agccatgctc ggagccagat
     1861 cgctcggtcg ccgagtgccc tgcgcgacct cgagcgcctg gttcggccgc gtcgattcag
     1921 cgccttcgct tgacgggggc gtggttacgg gctcttcgct gggttggcga agcgatcggc
     1981 gccgagtcac ttccatcggc agggtttcga gcgcccgctt cacttcctcc gggttgcccc
     2041 gtttggggaa agtcgcggtt cgcgcgactc ccttggcggt atcaagcaaa tcgagcggcg
     2101 cctccggcgc cagcgaaaca tcgaaatgca gcaccttgtt cccggcggcg atcgccgtaa
     2161 tggcacgcgg cacttgcacc ggctcggacc catcggctgc ctggccacgg cgccgcaatg
     2221 cttccgtgaa ttgcagttcg actgccggcc atccaccgat ttcaacgtac tctatcgtcg
     2281 catcgcgcga ggccgcgatg tccgcgagcc tcttcaatgc atcctcatga ctggtgcgcg
     2341 gctcggtcgt gaccagcatg tgcgcaagca ccgcggcggc agcggctttc tcttttcttc
     2401 cgagcgcctt gcccgtcacc acatactctt gggcgttgcg gtaccgcacg ccggatgccg
     2461 tccaagggcg cgccggtttg atggagaatc caggagcggc acttgcctgc gcagcttcgc
     2521 cgctttggct tgccgtgagt atgccgagca aggagagcgg caaggcggca tgaataacca
     2581 ggagcacggt gatgcgcgcc cggcgtgtca agttcttcca catggcgatc tccgctcatg
     2641 agccaggaaa acgggacaga gctcgtctgc gttttctcgg ctatgttggc gcgatagtgg
     2701 acgcgcccca atgcgccgtc tattactctc ggtctgtttg acaacgcgcc gcgttcagat
     2761 ttaagtttct cgccacttcg ataattcatc aactatgggt cgaaatcgac ccgggtcaac
     2821 ttcgacccgg aatcttcagc gacccgggtt tcatggatat tctttctcag ctcgccatca
     2881 gcgcgctgga tgatgcgctg agcggttttc tgccgagtac cgtgccgagc ggacttacgc
     2941 gcagggtgcg cgtggtcccg tccgaagtgc gcgcctcggg gctgggcggt tacgtcggca
     3001 cgcatccgga accgcgcggc gcggtgctgg cgcggcgcgt gcgcgccacg ctcgagcttg
     3061 ccgtgaccgg tggcaacgac gcggacgctt cagggtatct caatcaggtg gtgcgcacac
     3121 tcctgtcgca gcagcaaggc gacctgcgcc gtgagggcat ccagcgcatc acgctcaatg
     3181 ctgccgcggt gcaaccgcgc agcgccacgc tcgagctgga ttatgaatat cgccatctac
     3241 ccaccgagag cgaaggggtc atcgaactcc tggatctgaa tctcgacgtc aacctgacgc
     3301 cttaccgcgt gaagtaccgc tgggacctcg ccacgcgcac gctggtgggc gcgtcgcaac
     3361 cgctggcgga cttccaggtg gccgacgatc ccgacctgaa cagcggatcg cccgcttcgc
     3421 aatggacgtt caacgaggcg caggcgcgta tcgagcagaa cgcgctgacg cgtggcggcc
     3481 cgctcgccct gtcgcaaccc aagaaggcgg gtgcgcaact gctctggcgc cccggcggcg
     3541 aggccttcga gctcggccgc ttcatcgccg ccgtcgattt cgagtccggg agctccgacg
     3601 gcatcggcgt ggtgttcggg cgcaaggacg cgcagaactt ctggtacttc ctcgcctccg
     3661 agcgccaccg gtaccatctg ttcgggcgca aggacgccgg cgcctatagc ttcgtcggca
     3721 cgcctgccgg cgacgtcggt ttcgcactga acagccgcca cgtgctcgtg ctcgtggtct
     3781 acgaccagac cctgatcgcc gagctggacg gtgtgcgcac gctgaaggcc gaagcggatg
     3841 tgcaggtcga cgccggcgag atcggctttc tcacccacgg caacaaccag gcgcgcttcc
     3901 atcgtgcccg cctgatcgaa ctcgtttagg ggcccaccgg aaggaggacg gcaatggaag
     3961 acagttttgc tgagcagata gtccccggaa cattcattcg cgtgcaagcc gaggcgctga
     4021 tcgcgcccaa gggtgtggcg ttcggcaacg tcgggatcgt gggcaccgcg gccaacgccc
     4081 agggtgccag cgcgctcggc accacccaga tcatctccag cctcgaggac gccacccggc
     4141 tgtatgcgcc ttccgatgcg ctcagcgctg ggacgctgaa tctcatgcgc gggatggagt
     4201 tgctgttccg caacggcgcc aacacggtgt atgcccgcgg cctcgcacag ggcgccacgc
     4261 agcaggactt cgccaacgcc gtggacgagc tgctgaagga cgagatcaac ctgctggtcg
     4321 cgcccgagct caccacctcg gtcgccacca cggtgttgaa ggcggcggtg aacagcgccg
     4381 aaaccaacgc ccgcgatgtg atcgcagtga tcggcgccga cgcgaccacc gtggccaaca
     4441 tcgaggacca ggtgcaggcc gacaagcgcg tggtgctgtg cacgccgggc gtgttcgcgt
     4501 tcgacgcggc cgcgggcgcg gaggtgtcgc tgcccggcaa ctacaccgcg gcggcggtgg
     4561 ccggactgat cgcctcgctc gccccgcaat cgagccccac caacaaggtg ctgcccggga
     4621 tcaccaagct cggcatcaag ttcggctacg gcgagctgaa ggccctgctg caggccaacg
     4681 tgctgccgct cgagagccgg cagggtatcc gcgtggtgcg cggcatcacc agtgaaggcg
     4741 aagctttcgc tcagatcacc acgcgccgga tcgtcgattt cgcgaaggcc ggcatccgtc
     4801 aggtggccaa tccgttcatc ggccggctca acaacgaacg cgtgcgcaag gcgctgcagg
     4861 gcgcgctgga cggcttcctc accaccatgc tggtggacga agccctgacg cactacacgg
     4921 tggaggtcaa agccacccgg gccgacgaga tcgcgggccg ctgcgtggtg aacgtggtca
     4981 tgcagcccac cttcagcatc gactacatcc gcgtcacctt ggcgctgtcg taaaccatca
     5041 gggtcactgc catggcaaac accagcgtat tcaccggagc cgacggctcc atcacgttgt
     5101 ccaccacctc ctccgcgggc agagaagccg aggtcgccca gggcatcatc gacggcaacg
     5161 atctgatcac cgtgggccgg gccaccggcg tcgcggtcga ggtcacctcc gagatccacg
     5221 ccttccacga gatcggccag cgctacgcca ccgagctgcg cccgggcaac gtcgccgtgc
     5281 acggcaccat caagcgtgcc tatctcaacg gcgcgatgct gcgcctgctg ctgggcgaag
     5341 ccgccgacgg gcgcccgtcg aactcctggg cccagcccag cttcaacatc acgctgctgg
     5401 cggagaacgc cgcggtgccg ggcgtgcgct ccaccatcac gctgcacgga gtgaagctgg
     5461 accagtggag ctacaacgtg ccggaggacg acttcatcgt cgagtcggcg agcttccagg
     5521 cgctgttcct gacggtggcg gacgaaggcg cctgaggcat cggccagcgc aatagtttga
     5581 ctccgccggc cgcggccgca gctgcgtggg cgcggcggcg ggttcttccg gctgtgaact
     5641 ggagcgacga ccatgctgac agcagacgaa cttctcgccg gcgccaatct cacgttcgac
     5701 atcgaattgc cgccgcatgt cctcggcggg tccgggcctg cgcctgccgg cgccgtgcgc
     5761 ctgcgccctc tcacggtgcg cgacctgcaa ctgatcgcac gcgccgccaa ggacaacgat
     5821 gcactggccg gcgcgctgat ggtgcaggcc gcgctggtcg agcccaagct cacattgccg
     5881 caggtcaacg cgctgcaggt gggtctgctg cagttcctgc tgaccgaggt caaccgcgtc
     5941 agcggtatcg gcgccgatgc ggtcagcgtg cgcggcgccg cggaggaccc gctgatccgc
     6001 gccgcgttcc tgctggcgcg cgagttcggc tggacaccgg agcaggtcgg ggagctgacc
     6061 ctcggccaga tcctgctgca tctgcagctg ctgaaggagc agcgggtggc caatgcttga
     6121 agggaaacgg caagcagagc aggactgggc ggagtcgatc gcgccgctgc gacaagcggg
     6181 gcaagcgttc ggcgccgccg gcgagctcgt gcggcagctg gacgacaagg ccacgctgtg
     6241 gtcctgcgtg cgcgccctcg acgaggttcc ggcctgcatc acctttcatc cgttcacggc
     6301 cactgcggac tggtccgtgg acctgcagcg cgagcaggcg cacgcagtcc gggcggagca
     6361 gttgcagagc tggtcgcccg agctgatgcg acgcgcgcgc cggcggcagc agatctcggt
     6421 ggtgggcggc gctgcagcgc tctcgccatc cgccgccagt ccgccacgcg cgctgaaagc
     6481 ggattccacg ctcgcggatg cgcggctgcc cggcgcgcgc tccgctgcgc aggtcccgcg
     6541 aggcctcatg ccggaagccg gcgtctggaa cgcgctgctc gcgatcgtgc aactgatcga
     6601 cgagatcgag acggcgccgg aaccgggtga tgtgccgacc gccgtgcccg gagcgtcggt
     6661 ctcttcacaa ccgcgcagcg acaacgcgga cactgccgac atcccgccgg aaccaaaggc
     6721 caccgccgtt gccgcaacgg cgctgctatc gccgcccggg aagatcgccg cagcgaactc
     6781 cacgagcgct gcgggttcgg agccccagcc tgcgaccgcg ttgctcgggc agtacgtggc
     6841 ggcattgtgg agcggcgccg ctgcaccgtt gagctcgcga tccacggcag cgcacacggc
     6901 ttcgcgcgcc gcggtcgaac cggcttccgc ggcccgacca cgggcgccgt cgctgctgtt
     6961 gcccgcacgc gcgccgcgcg aaacgccgcc cggcccggaa ccggaggcac gctccgctga
     7021 cggcagctca cctgcgaccg cgtggccggc gaacgatccc ggccacgagg atgcgctggc
     7081 cgagcggctc aatcgcgcct tgctcgagca ggcctggttg agaggggtgg acctgacatg
     7141 agcccggggg tggatctgat atgagcctgg tgcccatgct cggcgactgg gaagtgccca
     7201 gcgtcacgcg gctggagacg ctggagagcc gtgcgctggc ggaaatggag atccccggcc
     7261 gcgcgggaaa cctgttccag gacctgaatc gcaagcccgc gcgcgtggtg gtggaagggt
     7321 cggtgttcgg tcaggacgcc ggccaggaat tcctcacctc ggtgcgcgag aaattcgtcg
     7381 ccggtgagcc gctcacgttc gtgtccgaca tcgccaccgg caccgaaatc cagcatgtcc
     7441 tggtgcagca gcttcatgtg caggctgcgg cgtcccacgc cgaccagatc gactacacct
     7501 tgtggttggt ggaatgtccg ccaccacccc cgcccagcga catcctgggc ggcatcgaca
     7561 ccggcctgct ggaccaggcg gcgggcatgc tcgacaccgc gctcggcgcc atcgacgcgc
     7621 tcgaagcgct gggcagcgta cccaatctgt ccgacccgac gaaaccgctg gcgggcgcac
     7681 tcgacgatgt gaagggcgcc gtggaccagc tcggggaggt gggcgcatcg ctgcaaggtc
     7741 tgttcgggtg acgcatgccc acgctgctgg aacaactgca atcggtcacc ggcggatcca
     7801 acgtcacgct ggacgtcgcc gcgcaggcgg gccagttcac ccagatcgcg caattggtgc
     7861 agcagctcgc gagcgagccg cccggcgact tcggcggcta tctgagccag ctgcaggggc
     7921 ttgcgctgcc caacgtctcg ctcggcggcc agctcggcca gtcgttcgga cagctgctgc
     7981 ccgacctgca aggcgggctg ggtggcatgc tgcagccgct gctggattcg gcgggcaaca
     8041 ttcagcaagg tgtcggcgcc gatctggccg gtgcgctcgg cccgctgctg gaggcgatcg
     8101 caaagctgcg cgacctggtg gcgagcgatt ggacctgcgg tctggtgcct gcgctcgccc
     8161 caccgccggc acccgagccg ccgccgggac ctccgccacc cgaacctccg ccgccgggac
     8221 ctcctcccgc cccgtcgacg ccagcgccgg ttctcactgc cgcccaggtc acgcaggcca
     8281 aggcggtgct cgacacgctg cccggcgaca tgagcgtcga ggcgctgctc aaatggctgc
     8341 accagcgcgt cggcaccttc cgcccgggcc gctacttgta tctgcgctcg ataccggtgc
     8401 tggacgacct gcgcgatccg ctcgacacgc tggtgcgctg ggacggcttc accggagcgc
     8461 agctcgccca ggagctggcg caaaccctga acgcgatggc cgcgatcatc acggccaaca
     8521 ccaccgacat cgccggcggg cctttcttcg ccggcacggt gcaggcgata ccggcacagg
     8581 ggttcgccac cgcgggcgat gcgctgggcg atgcgctcga cgcgctggcg gcggcggtgc
     8641 aggctcagaa cgcggcgacc atcgcaacgc agctcgccgc tgcacagagc gcggccacgc
     8701 aagtggaggc cgccaacgtc acgctcgacg cgcaacaggc ggccgtggag cagatggaag
     8761 agatggcgcg ggcgctgccg tcggcgctgg aaaccaccat gtgccggctg ctggtgctgt
     8821 cgcaaccgcg cgccacctgg ggcgatctgt cgaagcgctt cggtacgccg accgtgcttc
     8881 ctccggacgc cttcgcgccg ctcaccgacc tgttcgatcg cttgcaggaa ttcctcgacg
     8941 gcgtgctcga tgcggtggac atcggcgccg tgacgcagcc gatcgcacag gcgctgacgc
     9001 aggtcacgca ggccatccag cagctcgagc aggggcttgc gcagctcacc gccagcgcgc
     9061 gcgccgcctt cgagcaggcg cacaccgccc tgcaggcgct cgatctggag gcggtgctgc
     9121 agcaggccga ccagtcgctg caggcggcgt tgcagcaggt acagcaggcc attgcgcaag
     9181 gcctggcgcc ggcggccacg gcattgagcg atgcgctcac cgcggttgac ggcgcgctcg
     9241 ccggcttcga ccccgagcag ctggcccagc cggtgcagca ggtgatggac gcgatcaagt
     9301 cggtgttcga ggacgcggcc gtgcagcagg cgatccagca gctggagcgt ctcaaggaaa
     9361 tggcggacaa gctcgacgag ctgtcgttcc gtccggtaag cgacgtcgtc atcggcggca
     9421 tcggcacggt gaagtcggcg ctcgaagcga tcgacgaggc cagcctcccc gcgcccggtc
     9481 cagagctgat cagcgaagcc atgagcgtgc tgcccgagtc gctgacaccg ttgaccgacc
     9541 cgctcacctc caatctcgac gagctgatcg agaaaggacc gatcccgctg ctggaggccc
     9601 tgcgcgacct gcccaagccg atcgtggagg agatgcgcaa tttctcgccg cgtgggctgc
     9661 tcgaggaacc cctcggcacg ccgttcaggg aactgcgcgg acagctcgac gagttccagc
     9721 cgacacagtg gctggacgcg gccgagcagg agctcgacag cttgaagcag cgcctgttgc
     9781 aggcgctcga cctggaagcc ctgctggcgc cgctggcgca ggcgcagcag gcgctgctgc
     9841 aggaactcgg ccgcttccgc ccgggtgcgg tgatcgagcc gctcgcgcag cagctcgagc
     9901 aggcgctgga atcgctcgac gtggcgctgc cgaccaccca gatcacgcag gagctggacc
     9961 aggtgctggg acgcgtacag agcatcctcg gcactttcga tcaggtcgtg gaggtggtcc
    10021 aggcgttcac caccaagctg gcgctgctgg ccgacccgga tgcgcagctc gatcaatggc
    10081 tgaccgagat cttggccaag ctgccggccg acgcacccgc gaccttgagc gcgccgctcg
    10141 ctgcgctggc gcaagcggtg gacaatgcgc gcgcgacacc gctgcacacc gcttatcaga
    10201 gcgcgcgcca gcctctggcc gacaagctcg ccctggccga ccccgcggcg ttgctggtgc
    10261 gcatcctcca agggcgctcg cgcctgacgc cggcgctggt gaacgccctg ccggcttcgc
    10321 aggccaagac cgatctgcag caattcctga cgcagttcga tcccgccggc cccggcttca
    10381 gcagagggct acgccggctg gccggcctgc gcgacgcatt gagtgccgcc gatcagcaga
    10441 tgaccacgct gttcgccgaa tgggacgcgc gctatcaccg cagcgacggc gtgctcgcgg
    10501 cgctggtgca tcagtcggtg acggtggacg agctgcgcgg gtggctgcgc gaggcggtcg
    10561 acaagcagct cggcgcgccg gtcgtgggtt tcctcaagca gctcaagctg gtcggcacgc
    10621 tgctcgggac gttcggcgac gcgatcgccg gcgtggtgca ggcggtaaag gacaagatcg
    10681 acgatctgct ggccgtgccg caagcgctgt cgcaggccgc ccatgagctg gaacagttgc
    10741 agcaacagat caccagcctc gacctggaca tgttcgtgcg cgaggtcgac agtctgtacg
    10801 aggagctgct ggacacgctg cgcgcgctcg accccggcgc gatggagcag cccctgcgcc
    10861 gcgcgctcga agcgctgctg gacgacctca cgctcgactc ggtgttcacc ccgggcctgc
    10921 gcgatcagat cgagcagagc tatgccacgc tggtaggcaa agtcgacgcg ctggatcccg
    10981 agctgctggt gatcaagccg ctggatgacc tgtacacaca ggacgtcctg ccgctggtcg
    11041 atgcgctgga cgtgagcgaa gcggtgcaga agctgatcga tcggctgaac gcgctgcccg
    11101 acgagctgaa ggaggagctg gggcgggtcg acgaggccta tcaggacatg ctggcggccg
    11161 cgccgagcgg agcgccgtcg agcggcagcg cctccgtaag cctctagagg aaaagcggcg
    11221 atggcactcg gatcactgct gctgcaaccg aagacgctgg agcgcatgca gacgctggac
    11281 gccaatcagg cgttggccga cgacatcgtc gcgctgctgc atcagatcct ggtcaacccg
    11341 gaccacctgg acagcgtcgc gcccgcgttc cgcaaggcct tcgggggctt gggcgagcgc
    11401 atgcgcgggc gcctcgagtc gcgcctgcag agcgcgctcg accagttgcg cgatctgctc
    11461 ggcccggtcc tccaggccgg gcaggacgtc gcggcgaacg gacagcacct ggactcggcc
    11521 ggcgaggtgt tcgagctgat cgccacggcg ctcgacaagc tgatcgcggc gctgcaggcg
//
LOCUS       DASPGE010000099        10515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_264723, whole genome shotgun sequence.
ACCESSION   DASPGE010000099 DASPGE010000000
VERSION     DASPGE010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10515
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264723"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /locus_tag="VD791_03155"
     CDS             complement(<1..117)
                     /locus_tag="VD791_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV06999.1"
                     /translation="MELKGKQALVLGLGVSGLSMARWLASRGAHVRVADTRAT"
     gene            complement(265..420)
                     /locus_tag="VD791_03160"
     CDS             complement(265..420)
                     /locus_tag="VD791_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07000.1"
                     /translation="MAHDLVSAEANLRSKLSRALRGGRSQNQVVVRFWIITMMLVLVG
                     LSTLKLR"
     gene            complement(426..1511)
                     /gene="mraY"
                     /locus_tag="VD791_03165"
     CDS             complement(426..1511)
                     /gene="mraY"
                     /locus_tag="VD791_03165"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HXV07001.1"
                     /translation="MLLELAQWAAQEVRAFNVFNYITLRAVLATLTALVISFIVGPVM
                     IRRLTAYKIGQAVRDDGPQTHLTKAGTPTMGGALILVSIAITTLLWTDLTNRYVWVVL
                     AVTTGFGLIGWVDDYRKVVHRNPKGLSARAKLFWQSVIGLTAAVYLAFSTTLPAQTEF
                     IVPFFKHVAYPLGVWGFILMTYFVIVGTSNAVNLTDGLDGLAIMPTVMVGSALGVFAY
                     VAGHAVFSKYLGFPHIPGASELTVFCAALAGAGLAFLWFNAYPAEVFMGDVGALALGA
                     ALGTLAVICRQEIVLFIMGGVFVVETLSVMLQVASFKLTGKRIFRMAPLHHHYELKGW
                     KENQVVVRFWIITMMLVLVGLSTLKLR"
     gene            complement(1514..2875)
                     /gene="murF"
                     /locus_tag="VD791_03170"
     CDS             complement(1514..2875)
                     /gene="murF"
                     /locus_tag="VD791_03170"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HXV07002.1"
                     /translation="MLDLDTAAREIGAGRRGPNLSFDGVTTDSRQVARGDLFVALRGE
                     RFDGHAFADQALAAGAVAAMVEDPDRVATRGASLILVDDTLQGLGRLAGAWRARFAPA
                     LAGVTGSNGKTTVKEMIAGILRAQAGDEAVLATSGNLNNAIGVPLMLLRLRSHHRYAV
                     IEMGMNHLGEIRYLTKLAAPTVAVVTNAGTAHIGEVGSREAIAQAKGEIYEGLRDRTA
                     VINADDRFAGYWRGLNAGRGIVDFGFEQPAAVRGSLSGSVLSVRTPDAAYELRLRVPG
                     AHNAHNGLAACAVACALAMPPAAVVRGLQEYEGSRARLQRRAGTGGSLVIDDTYNANP
                     DSTRAAIAVLAAQPGTRILVLGDMGELGSEGPALHAEVGAEARRAGIDRLFALGPLSA
                     ETARAFGSAAHHFTEVDALVSELSPLLRAGVTVLVKGSRFMRMERVVSRLADGEPVAA
                     GDH"
     gene            complement(2880..4334)
                     /locus_tag="VD791_03175"
     CDS             complement(2880..4334)
                     /locus_tag="VD791_03175"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HXV07003.1"
                     /translation="MKPAGRFDPAALAALGVEVKRLRSDSRQVQAGDVFAAFPGERTD
                     GRRFIPQAIAAGAAAVLWESRAFQWDPSWRVPNLGVAELRNEVGEIAAYVYGHPSRQM
                     WVAGVTGTNGKTSCSHWIAQALGRLGRPTAIIGTLGIGFPGALAPATHTTPDAVALQE
                     EMVGLLARGARCVSMEVSSHGLDLSRVNGVAFSAALFTNLSRDHLDHHGSMRRYGAAK
                     AKLFRWPGLEHAVINLDDAFGRELAASLDRSRVNVLGYGLGKGEISGHRLDLSKRGLS
                     LEIETPWGAGAIRSRVLGGFNASNLLGVLGVLLAAGVQLDQATASLAEVEPVAGRVQL
                     IREAGKPLVVVDYAHTPDALEKVLETLRPLLPDGAQLVCVFGCGGDRDPGKRPLMGEV
                     ATRLAHRTLVTSDNPRSEDPLAIIGQVIAGAHPTYQVDADRASAIHRAVHAAGPEDIV
                     LIAGKGHETYQEIAGVRLPFNDAEVAQAALRARN"
     gene            complement(4331..6073)
                     /locus_tag="VD791_03180"
     CDS             complement(4331..6073)
                     /locus_tag="VD791_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXV07004.1"
                     /translation="MKPVNPALSLKLPIARARVLLGLLLLGFATLAGRALHLQALQND
                     FLQRKGESRYARLLEMPAHRGMITDRHGEPLAISTPVESVWASPADVEATPAQRRQLA
                     RVLNMSEADLNKRLSGSGREFVYLKRHLRPDVAARLVQLGIAGVSLKREYRRYYPAGE
                     VTAHLLGFTDVDDRGQEGIELAYDDWLTGKPGSRRVIKDRLGRVVEDTESVLAPIEGR
                     DVVLSIDRKLQYLAYRELKAAAAEHRAKAGAIVVLDVKSGEVLALANVPAYNPNNRSR
                     RDAARARNRAITDLFEPGSTLKPFTIAAALEARLIRPESTVQTAPGYLLVGNARIDDV
                     HPAQVLTVSELIQRSSNVGVAKLALALPRQQLWSVLSAAGFGAPPRSGFPGEVGGRLR
                     DYQRWRPIEQATLSYGHGISVSLLQLARAYTVFAGDGALQPISLLKRDAPGAATRVIS
                     PGTARAVRAMLETVVHPGGTAVRAQVPGYRVAGKTGTAHKVQGSGYSPDRYVSSFVGM
                     APASDPRLLVAVMIDEPDNGDYYGGVVAAPVFSSVMAEALRILSVPPDAPATLVEPGE
                     LQPNGPLPEVREEV"
     gene            complement(6070..6348)
                     /gene="ftsL"
                     /locus_tag="VD791_03185"
     CDS             complement(6070..6348)
                     /gene="ftsL"
                     /locus_tag="VD791_03185"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HXV07005.1"
                     /translation="MIRINLVLLCLLVICALALVTSQHRARKLFTELEKEQDIGKQID
                     VEWGQLQLEQSTWATHSRVEHIARGTLRMRVPPSGRVRLVPAPARSVP"
     gene            complement(6345..7274)
                     /gene="rsmH"
                     /locus_tag="VD791_03190"
     CDS             complement(6345..7274)
                     /gene="rsmH"
                     /locus_tag="VD791_03190"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HXV07006.1"
                     /translation="MSVPAHRPVLLLEAIDALNVKEDGIYVDCTFGRGGHSRAILERL
                     GARGRLVALDRDPQAVQAGAEIRDPRFSIHHTRFGRMAEVLAAEGVARVDGVLIDLGV
                     SSPQLEDAARGFSFRLDGPLDMRMDTTRGITAEQWLASASEDHIREVIETYGEERFAG
                     AIARAIVAARSRGAVRTTGQLAQVVGQAVRTREPGQDPATRTFQAVRIFINQELEELP
                     LALAQALDHLAAGGRLAVISFHSLEDRLVKRFLRSHSQPAPVPRGLAVRESERAPAKL
                     RLVGKPRRPGAAEIALNPRSRSATLRVAERLAA"
     gene            complement(7271..7717)
                     /gene="mraZ"
                     /locus_tag="VD791_03195"
     CDS             complement(7271..7717)
                     /gene="mraZ"
                     /locus_tag="VD791_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HXV07007.1"
                     /translation="MFRGVTALTIDSKGRVPMPARYRDMLQARAGGKLVITADSALCL
                     LVYAAPDWEPIQEKLMSLSSFHPRVRDLQRLLVGNASDAEMDAAGRILIPGPLRRFAG
                     LEKDVALVGQGARFELWDERKWAQQMEQSIALTEDGVPPELEGFSL"
     gene            complement(7998..8360)
                     /gene="rnpB"
                     /locus_tag="VD791_03200"
     ncRNA           complement(7998..8360)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VD791_03200"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(8564..9496)
                     /locus_tag="VD791_03205"
     CDS             complement(8564..9496)
                     /locus_tag="VD791_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXV07008.1"
                     /translation="MSSPGHRRNDAARGARLAAFAWLSVAAAVATIALKGLAYWLTGS
                     VGLLSDALESFVNLAAAIVALTMIHVAARPPDEEHAYGYSKAEYFASGFEGALIFLAA
                     VAIGVAAGQRLIAPVPLERLDAGLAISAGAAALNLAVARVLTRAGRRYDSIALEADGR
                     HLMTDVWTSVGVIAALFAVLLTGWQWLDPLIALAVALNILWTGYRLLKRSALGLLDRA
                     LPQPQRAAIAQVLARYQRDDLQFHALRTRQAAGRSFISVHVLVPGAWSVQRGHDLLEE
                     IERELRAAVPGAAVFTHLEPVEDPASFLDRELDR"
     gene            complement(9534..>10515)
                     /gene="pyrC"
                     /locus_tag="VD791_03210"
     CDS             complement(9534..>10515)
                     /gene="pyrC"
                     /locus_tag="VD791_03210"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HXV07009.1"
                     /translation="DGAHMQAVLAHSARRFARAIVMPNLRPPITTTGLALEYRARILA
                     ALPAGASFRPLMTLYLTDDTQPEEIARARASGAVYAVKYYPAGATTHSDSGVTDLRRC
                     ADTLQAMTEAGMPLLVHGEVTDAAVDVFDRERVFIERVLRPLIERYPQLRIVLEHITT
                     SEAVRFVESAPPNVAATVTAHHLLLNRNAMFQGGIRPHHYCLPVLKREEHRRALLQAA
                     TSGSPKFFLGTDSAPHARHTKEHACGCAGVYTAHAGIELYAEAFEEAGALPRLEAFAS
                     LHGADFYGLPRNSERLALRRNRWAVPEDLAYGEDRLVPLRAGGSVAWSLD"
BASE COUNT         1646 a         3701 c         3597 g         1571 t
ORIGIN      
        1 cgtggcgcgc gtgtccgcca cgcgcacgtg cgcgccgcgg ctcgccagcc agcgcgccat
       61 cgacaggccg ctcactccga gcccgagcac gagtgcctgt ttgcctttca gttccacgct
      121 actgcttacc ccttaaccgc aaaggcgcga aggcgccaag gaaacgcaaa gacgtccacg
      181 cgaagcgcac gaacgaataa cgatgagtcg tttcctttgc gtcatctttg cgtctttgcg
      241 cctttgcggt cctcgtcatg cgcgtcagcg cagctttaaa gtcgacagcc cgaccaatac
      301 gagcatcatg gtgatgatcc agaagcggac gacaacttga ttttggctgc ggccgccgcg
      361 caaagcgcgg cttaacttgc tgcgcaagtt agcctccgcc gaaaccaagt cgtgggccat
      421 ttttctcacc gtagcttcag ggtcgagagc ccgaccaata caagcatcat cgtgatgatc
      481 cagaagcgga ccacgacttg gttttccttc caccccttca gctcgtagtg gtgatgcagc
      541 ggcgccatgc ggaagatgcg ttttccggtg agcttgaagg aggccacctg cagcatcacc
      601 gagagcgtct cgaccacgaa caccccgccc atgatgaaca gcacgatctc ctggcggcag
      661 atcaccgcca gggtgccgag cgccgcgccc agcgccagcg cgcccacgtc gcccatgaac
      721 acctcggcgg gataggcgtt gaaccacagg aaggcgagcc cggcaccggc cagcgccgcg
      781 cagaacacgg tcagctcgga ggcgcccggg atgtgcggga agccgagata tttggagaac
      841 accgcgtggc cggcgacgta ggcgaacacg cccagggcgc tgcccaccat caccgtgggc
      901 atgatcgcca ggccgtccag cccgtcggtc aggttcaccg cgttgctggt gccgacgatg
      961 acgaagtagg tcatcaggat gaagccccag acgcccaacg gataggccac gtgcttgaag
     1021 aagggcacga tgaactcggt ctgcgccggc agcgtggtgc tgaaggcgag gtacaccgcc
     1081 gccgtcagcc caatcaccga ctgccagaag agcttcgccc gggccgacag gcccttggga
     1141 ttgcgatgca ccaccttgcg gtagtcgtcc acccagccga tcaggccgaa tccggtggtc
     1201 accgcgagca cgacccagac gtagcggttg gtgaggtcgg tccacagcag cgtggtgatc
     1261 gcgatcgaca ccaggatcag cgcgccgccc atggtcggcg tgccggcctt ggtgaggtgc
     1321 gtctgcggac cgtcgtcgcg caccgcctgg ccgatcttgt aggcggtcaa cctgcggatc
     1381 atgaccggcc cgacgatgaa ggagatcacc agcgcggtga gcgtggccag caccgcgcgc
     1441 agggtgatgt agttgaacac gttgaaggcg cgtacttcct gcgccgccca ttgcgccagt
     1501 tcgagcaaca tgtctagtga tctcccgccg ccaccggctc gccgtccgca aggcgcgata
     1561 cgacgcgttc catgcgcatg aagcgcgacc ccttcaccag caccgtcacg cccgcgcgca
     1621 gcagcggcga caactcgctc accagcgcgt ccacttcggt gaagtgatgc gcagccgagc
     1681 cgaaggcgcg ggccgtctcg gccgacagcg ggccgagcgc gaacagccgg tcgatgcccg
     1741 cgcgcctggc ttccgcaccg acctccgcgt gcaatgccgg accctccgac cccagctcgc
     1801 ccatgtcgcc cagcaccagg atgcgcgtgc cgggttgcgc ggcgagcacg gcgatggcgg
     1861 cccgcgtcga atccgggttg gcgttgtagg tgtcgtcgat caccaggctg ccgcccgtgc
     1921 cggccctgcg ctgcagccgc gcccggctgc cctcgtactc ctgcaggccg cgcacgaccg
     1981 ccgccggcgg cattgcgaga gcgcaggcca ccgcgcaggc ggcgagcccg ttgtgcgcgt
     2041 tgtgcgcgcc cggcacgcgc agccgcagct cataggcggc gtcgggcgtg cgcacggaca
     2101 ggaccgaccc gctcaagctg ccgcgcaccg cggccggctg ctcgaagccg aagtcgacga
     2161 tcccccggcc ggcattcaag ccgcgccagt agccggcaaa gcgatcgtcg gcgttgatca
     2221 ccgcggtcct gtcgcgcaag ccttcgtaga tctcgccctt cgcctgcgcg atcgcttcgc
     2281 gcgaaccgac ctcgccgatg tgcgcggtgc cggcgttggt caccacggcg accgtgggcg
     2341 cggcgagctt ggtcaggtag cggatctcgc ccaggtggtt catccccatc tcgatcacgg
     2401 cgtagcggtg gtggctgcgc agccgcagca gcatcagcgg cacgccgatc gcgttgttca
     2461 ggttgccgct ggtggcgagc accgcttcgt cgccggcctg cgcgcgcagg atgccggcga
     2521 tcatctcctt gaccgtggtc ttgccgttgc tgccggtcac gcccgccagc gccggagcga
     2581 aacgcgcgcg ccatgcgccg gcgagccgcc ctagcccctg caaggtgtcg tccaccagga
     2641 tcagcgaagc gccacgggtg gcgacgcggt cggggtcttc caccatcgcg gccaccgcgc
     2701 cggcggcgag cgcctggtcg gcgaaggcat gaccgtcgaa gcgctctccg cgcaacgcga
     2761 cgaacaggtc gccgcgcgcg acctggcggc tgtcggtggt cacgccgtcg aagctcaggt
     2821 tcgggccgcg gcgtccggcg ccgatctcgc gggctgcggt gtcgaggtcg agcacggccc
     2881 taattcctcg cccgcagcgc ggcctgcgcc acttcggcgt cgttgaacgg cagcctcacg
     2941 ccggcgatct cctggtaggt ttcgtggccc ttgccggcga tcagcacgat gtcctccggg
     3001 ccggcggcat gcaccgcgcg atggatcgcg gaggcgcgat cggcatccac ctggtaggtc
     3061 ggatgggcgc ccgcgatcac ctgcccgatg atggccagcg gatcctcgct gcgcgggttg
     3121 tcgctggtga ccagggtgcg gtgcgcgagc cgtgtggcca cctcgcccat cagcgggcgc
     3181 ttgccgggat cgcgatcgcc gccgcagccg aacacgcaca cgagctgcgc gccgtcgggc
     3241 agcagcggcc gcaacgtctc cagcactttc tccagcgcgt cgggcgtgtg cgcgtaatcc
     3301 accaccacca gcggcttgcc cgcctcgcgg atcagctgca cgcgtccggc caccggctcc
     3361 acctcggcca gcgacgcggt ggcctggtcc agctgcacgc ccgcggccag cagcacgccc
     3421 agcacgccca gcaggttgga ggcgttgaag ccgcccagga cgcggctgcg gatcgcgccc
     3481 gcgccccacg gcgtttcgat ctccaggctt aagccccgtt tgctgagatc gagccgatgg
     3541 ccggagattt cgcccttgcc caggccgtag ccgagcacgt tgacgcggct gcggtcgagc
     3601 gaggccgcca gctcgcgccc gaaggcgtcg tcgagattga tcacggcgtg ctcgaggccc
     3661 ggccagcgaa acagcttcgc cttggccgcg ccgtagcggc gcatgctgcc gtggtgatcc
     3721 aggtggtccc gagaaagatt ggtgaacagc gcggcggaga acgccacgcc gttcacccgg
     3781 ctcaagtcga gcccatggga ggagacttcc atcgagacgc acctggcgcc gcgcgcgagc
     3841 aggccgacca tctcctcctg caacgcgacg gcgtcgggtg tggtgtgcgt ggcgggcgcg
     3901 agcgcgcccg gaaagccgat gcccagcgtg ccgatgatgg cggtcgggcg accgagacgt
     3961 ccgagcgctt gcgcaatcca gtgcgagcac gaagtcttgc cgttggtgcc ggtaacgccc
     4021 gcgacccaca tctggcgcga cggatgtccg tagacgtagg cggcgatctc gccgacctcg
     4081 ttgcgcagct cggccacgcc caggttgggg acgcgccagg acgggtccca ctggaacgcg
     4141 cgcgattccc acagcaccgc ggctgcgccc gccgcgatgg cctgcgggat gaagcggcgc
     4201 ccgtcggtgc gctccccggg gaaggcggcg aacacatcgc cggcctgcac ctgccggctg
     4261 tcgctgcgca ggcgcttcac ctccacgccc agggcggcca gtgcggcggg atcgaatcgc
     4321 ccggcgggct tcatacctct tccctcacct ccggcagcgg tccgttgggc tgcaactcgc
     4381 ccggctcgac cagcgtggcc ggggcgtccg gggggacgga caggatgcgc agcgcctcgg
     4441 ccatcaccga gctgaacacc ggggccgcga ccacgccgcc gtaatagtcg ccattgtccg
     4501 gctcgtcgat catcaccgcc accagcaggc gcgggtcgga agccggcgcc atgccgacga
     4561 aggacgacac gtatcggtcc ggcgagtagc cgctgccctg caccttgtgc gcggtgccgg
     4621 tcttccccgc gacgcggtag cccggtacct gggcgcgcac cgcggtaccg ccggggtgca
     4681 ccaccgtctc gagcatcgcg cgcaccgcgc gcgccgtgcc gggagagatc acgcgcgtgg
     4741 ccgcgcccgg cgcatcgcgt ttgagcagcg agatcggctg cagcgcgccg tcgccggcga
     4801 acaccgtgta agcgcgcgcg agctgcagca ggctgaccga gatgccgtgg ccgtaggaca
     4861 gggtggcctg ctcgatcggc cgccagcgct ggtagtcgcg caggcgcccg ccgacttcgc
     4921 ccgggaagcc gctgcgcggc ggggcgccga agccggccgc ggacagcacg ctccacagct
     4981 gctgtcgtgg cagcgccagc gcgagcttgg ccacgccgac attggacgag cgctggatca
     5041 gctccgacac cgtcagcact tgcgccgggt gcacgtcgtc gatgcgggcg ttgcctacca
     5101 ggaggtaccc gggggcggtc tgcaccgtgc tttcgggacg gatcagccgc gcttccagcg
     5161 cggcggcgat ggtgaagggc ttcagcgtcg agcccggctc gaacaggtcg gtgatggcgc
     5221 ggttgcgcgc gcgcgcggca tcgcggcggg agcggttgtt ggggttgtag gccgggacgt
     5281 tggcgagcgc cagcacctcg ccgctcttca cgtccaggac gacgatggca cccgccttgg
     5341 cgcggtgctc ggcggccgcg gccttcagct cgcggtaggc gaggtattgc agcttgcggt
     5401 cgatggacag tacgacgtcg cggccctcga tcggcgcgag cacgctttcg gtgtcttcca
     5461 ccacgcgccc gagacggtcc ttgatcaccc gccggctgcc gggcttgccc gtcagccagt
     5521 cgtcgtacgc cagctcgatg ccttcctggc cgcggtcgtc cacgtcggtg aagccgagca
     5581 gatgcgccgt cacttccccg gccgggtagt agcggcggta ctcgcgcttg agcgagacgc
     5641 cggcgatgcc gagctgcacc aggcgagcgg ccacgtcggg gcgaaggtgg cgcttgaggt
     5701 agacgaactc gcgccccgag cccgacagcc gcttgttcag atcggcttcg ctcatgttca
     5761 gcacacgcgc gagctggcgg cgctgagccg gcgtggcttc gacgtccgcc gggctcgccc
     5821 agaccgattc caccggcgtg gagatggcga gcggctcgcc gtgccggtcg gtgatcatgc
     5881 cccggtgcgc cggcatctcc agcaggcggg cgtaacgcga ctcgcccttg cgctgcagga
     5941 agtcgttctg cagcgcctgc agatgcagcg cgcgcccggc cagcgtcgcg aagcccagga
     6001 gcaacaggcc cagcagcacg cgcgcacgcg cgatcggcag cttcagcgac agggccgggt
     6061 tgaccggctt cacggcacgc tccgtgcggg cgccggcacc agccgcacgc gccccgatgg
     6121 cggcacgcgc atgcgcagcg tgccgcgggc gatgtgctcg acccgcgagt gcgtcgccca
     6181 cgtgctctgc tcgagctgca gctgacccca ctcgacgtcg atctgcttgc cgatgtcctg
     6241 ctccttctcg agctcggtga acagcttgcg cgcgcgatgc tggctggtca ccagcgccag
     6301 cgcgcagatc accagcaggc acagcaggac gaggttgatg cggatcatgc cgcgagccgt
     6361 tccgccaccc gcagcgtcgc gctgcggctg cgcgggttga gcgcgatctc cgccgccccg
     6421 gggcgacgcg gcttgcccac caggcgcagc ttcgccggcg cgcgctccga ttcgcgcacc
     6481 gccaggccgc ggggcaccgg cgccggctgc gagtggctgc gcaggaagcg cttcaccagc
     6541 cggtcctcga gcgaatgaaa gctgatcacc gccaggcgtc cgcccgcagc caggtggtcg
     6601 agcgcctggg cgagcgctag cggaagttcc tcaagctctt gattgatgaa aatccgtaca
     6661 gcctggaatg tccgcgtcgc cgggtcctgg ccaggttcgc gcgtgcggac ggcttgaccc
     6721 acgacctggg caagttgtcc ggtggtgcgg acagctcccc gcgatcgagc cgcaacaatc
     6781 gctcttgcaa tcgcgccagc aaaccgttct tcgccatagg tctcgatcac ctccctgatg
     6841 tggtcctcgg acgcgctcgc cagccactgc tcggcggtga tgccgcgggt ggtgtccatg
     6901 cgcatgtcga gcggaccgtc gagccggaag ctgaagccgc gcgcggcgtc ctcgagctgc
     6961 ggcgaggaaa cgcccaggtc gatcagcacg ccgtccacgc gcgcaacgcc ttcggcggcg
     7021 agcacctcgg ccatgcgacc gaagcgcgtg tggtggatgc tgaagcgcgg gtcgcggatc
     7081 tcggcgcccg cctgcaccgc ctgcggatcg cggtcgagcg cgaccagccg gccgcgggcg
     7141 cccaggcgct cgaggatggc ccggctgtgt ccgccgcgtc cgaaggtgca atccacgtag
     7201 atcccgtctt ccttcacgtt cagcgcgtcg atcgcctcga gcagcaggac cgggcgatgg
     7261 gccggcacgc tcagagcgag aaaccctcca actccggcgg cacgccgtcc tcggtgagcg
     7321 cgatggactg ttccatctgc tgcgcccact tgcgttcatc ccacagctcg aaccgggcgc
     7381 cctgccccac cagcgccacg tccttctcca gcccggcgaa cctgcgcagc ggccccggaa
     7441 tcaggatgcg gccggccgcg tccatctcgg catcggaggc gttgcccacc agcagccgct
     7501 gaagatcgcg cacgcgcggg tggaagctcg acaggctcat gagcttttcc tggatcggtt
     7561 cccaatccgg ggcggcgtac acaagaaggc agagggccga gtccgcagtg atcaccaact
     7621 tacccccagc gcgggcctgc agcatatccc ggtaccgggc aggcatcggc actcggccct
     7681 tgctgtcgat cgtcagcgca gtgacgccgc ggaacacgca cccccctgat ttttagggat
     7741 ctcccacgat cccccacttt tacccaccgg acgccactat agtgaaaccg ccggcgatgg
     7801 tcaagcgaca gacggggttt ttcgcattcg cgacaaccac ttatctcaac gtcccggcaa
     7861 ctcgagagag caaaaagcag aaacattaaa aaagaatgag atagataaga tattgaaagt
     7921 tatttcgaga agaaaacgcc atcgatgcgt ctgcgcgagg cgacagatcg ggcgatttcg
     7981 gccgatgcaa aggaggcgtg cgaagccggc ctgtaagccg ggttctgtgc agggaaagcc
     8041 cctgcggcag tcattcctct aggcgaccgg ttgcccggac actcgagcga cctacccggg
     8101 agcagcgcga gccacgctgt cgctccccta tttggtcttg ctccggatgg ggtttgccga
     8161 ctcccgacgt taccgcggga gcggtgcgct cttaccgcac catttcaccc ttgcctgagc
     8221 cctggcgggc catcggcggt gtactttctg ttgcactttc cgtcgtctcg cgacgcccgg
     8281 ccgttagccg gcatcctgct ctgtggagcc cggactttcc tctcccgcct cgcgacggca
     8341 gcgactgccc ggccgacttc acacgattga gattgtaggc gtttagacaa aaaagtccgg
     8401 gcgccgtttc atgggagccc gaactttggc ttcggccgcc gcgcggtaca accgcgcggc
     8461 ttaaacttgg ctgaagccaa gttagcctgc gccgcaacag cttcgcggtt gtggcttcgg
     8521 ccgccgcgcg gtgcaacggc ttggtgagac ttgcgcgatt cagtcagcgg tccagctcgc
     8581 gatcgaggaa tgaggccggg tcctcgaccg gctcgagatg cgtgaacacc gcggcgcccg
     8641 gcaccgcagc gcgcagctcg cgctcaatct cctccagcag atcgtggccg cgctggacgc
     8701 tccaggcgcc gggcaccagc acgtgcaccg agatgaagga gcgccccgcg gcctggcgcg
     8761 tacgcaacgc gtggaactgc aggtcgtcgc gctggtagcg cgccagcacc tgcgcgatcg
     8821 ccgcgcgctg cggctgcggc agcgcgcggt ccagcagccc caaagcggag cgcttgagca
     8881 gccggtaacc ggtccacagg atgttcagcg ccaccgcgag cgcgatcaac gggtcgagcc
     8941 actgccagcc ggtgagcagc accgcgaaca acgcggcgat cacgcccacc gatgtccata
     9001 cgtcggtcat caggtgcctg ccgtcggcct ccagcgcgat cgagtcgtag cgccgcccgg
     9061 cgcgcgtcag gacccgggcg acggcgagat tgagggcggc ggcgcccgcc gagatcgcca
     9121 gaccggcgtc gagccgctcg agcgggaccg gcgcgatcaa ccgctgcccc gcggcgacgc
     9181 cgatggccac ggcggcgaga aagatgagcg caccttcgaa gccgctggcg aagtactccg
     9241 ccttgctgta gccgtaggcg tgctcctcgt ccggcgggcg cgcggcgacg tggatcatgg
     9301 tgagcgcgac gatcgccgca gccaggttga cgaaggactc cagcgcgtcg gagagcaggc
     9361 ccaccgagcc ggtgagccag taggccaggc ccttgagcgc gatggtcgcg accgccgcgg
     9421 ccaccgaaag ccaggcaaag gccgccaagc gcgcgccccg cgccgcgtcg ttgcgccggt
     9481 gtccgggcga gctcacccgg cccgtcgcct ctcccgcggg cgggagaggg cggctagtcg
     9541 agcgaccatg ccacgctgcc gcccgcgcgc agcggcacca ggcgatcttc gccgtaggcc
     9601 aagtcctccg gcaccgccca gcggttacgc cgcagagcga gccgctcgct gttgcgcggc
     9661 aggccgtaga aatcggcgcc atgcaagctt gcgaatgcct ccaggcgcgg cagcgcaccg
     9721 gcctcttcga aggcctcggc gtacagctcg atgccggcgt gcgccgtgta cacacccgcg
     9781 cacccgcagg cgtgctcctt ggtgtggcgc gcatgcggtg cgctgtcggt gcccaggaag
     9841 aacttggggc tgccggaggt ggccgcctgc agcagcgcgc gccggtgctc ctcgcgcttg
     9901 aggacgggaa ggcagtagtg gtgcgggcgg atgccgccct gaaacatggc gttgcggttg
     9961 agcagcagat ggtgcgcggt gaccgtggca gcgacgttgg gcggcgccga ctcgacgaag
    10021 cgtaccgcct cgctggtggt gatgtgctcc agcacgatcc tgagctgcgg gtagcgctcg
    10081 atcaggggcc tcagcacacg ctcgatgaac acccgctccc ggtcgaacac gtccaccgcc
    10141 gcgtcggtaa cctccccgtg caccagcaac ggcatgccgg cttcggtcat cgcctgcagc
    10201 gtgtcggcgc agcgccgcag gtcggtcacg ccggaatcgg agtgggtggt agcgcccgcc
    10261 gggtagtact tgaccgcgta cacggccccg ctcgcccggg cgcgcgcgat ctcctccggc
    10321 tgtgtgtcgt cggtgagata gagcgtcatc agcggccgaa agctcgcgcc ggcgggcagg
    10381 gctgcgagga tgcgggcgcg gtactccagc gcgagcccgg tggtggtgat cggcggtctg
    10441 aggttgggca tgacgatggc gcgcgcgaaa cgtcgcgcgc tgtgcgcgag caccgcctgc
    10501 atgtgtgcgc cgtcg
//
LOCUS       DASPGE010000100         5190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1084481, whole genome shotgun sequence.
ACCESSION   DASPGE010000100 DASPGE010000000
VERSION     DASPGE010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084481"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VD791_03215"
     CDS             complement(<1..1052)
                     /locus_tag="VD791_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV07010.1"
                     /translation="MTRLRPHLLRAAVGLLLLAIGLAAVLAWQKWREQPLEERYQLEA
                     VTTGDLAQTVTANGTVNPVGLVNVGTQVSGTVLRIHADFNDRVKAGQVLLELDPTTLR
                     ATVEQSSGELASAEATLRLTRQEETRMRELHAQEYVSRQELEKAVQAREAAQAQVRTA
                     QGKLARDRANLGYAVIRSPVAGVVVSREVDVGQTVAASFQTPTLFKIARDLSRMQIDS
                     NVAEADIGKVAVGQPVRFTVDAYPERRFEGRVHQIRLSPLTQQNVVTYNVVVEVANPD
                     LALMPGMTAYLAIVTDRAEHAVLVPNAALRFRPSEAKTGTGAAVAGNSDARRREARRP
                     GEAAVYVLRGEEIVRVP"
     gene            complement(1066..2505)
                     /locus_tag="VD791_03220"
     CDS             complement(1066..2505)
                     /locus_tag="VD791_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXV07011.1"
                     /translation="MPPLPFAPGRVACALLLALAPATALGQRGADPFETRKQLPAAPS
                     SFWSGPPGPAPCPDAATMPETLRLVEAIDLALCNNPRTRQSWAIARQAAAEVGVARSA
                     RLPTVDALIELERREVRNIDDPQGFTLLPASVGFSWLLFDFGGRDAAIALAAESLAAA
                     NWTHNATLQTVMLDAITAYYLLFATREAVDATVAAEKSALQSLEAARARLRAGSATRA
                     DVLQAQTAYSQAVFNRTQAEGEAARSQGVLANVLGISPDRPLSIAPPPDLEAQRAIER
                     SMGELLAAARAQRPELAAAEARVRAAESNVRVRESAAKPTVSLFGSAGATLSNPGPDP
                     VSGAIGLSVSIPIFTGYFNTYRIQQAREQVLEQAATRDRLADDVALEVWLAYQNLRTE
                     GQSLATANDLVESAQESYNVALARYRAGVGTVIDLLNAQSALSAALVQRIEARLRWNV
                     AKAALARAVGVLDPALVAQLSQSSAPLPR"
     gene            2828..3484
                     /locus_tag="VD791_03225"
     CDS             2828..3484
                     /locus_tag="VD791_03225"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXV07012.1"
                     /translation="MALRIGDEAPNFTAQTTEGTIDFHKWIGDGWAILFSHPKDFTPV
                     CTTELGYMARLKPQFDKRNCKVIGLSIDPVADHEKWARDIQETQGYAPNYPLIGDADL
                     KVAKLYDMIHPNASGEQKRTAADNATIRSVFVVGPDKKIKLILTYPMSTGRNFDEVLR
                     VLDSMQLTAKHKVATPVNWKQGEDVIIVPSVPDEEAKKQFPGGWKAPKPYLRIVPQLK
                     "
     gene            complement(3673..4671)
                     /locus_tag="VD791_03230"
     CDS             complement(3673..4671)
                     /locus_tag="VD791_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV07013.1"
                     /translation="MTHRKRPRALALLALLLPAAAVAGGSDYTVRPGSNAAFAGNARE
                     WPVPTPQFARDPAPGPDGAIYIAVMYGNKIARFDPKTETFKEWELPPGAKPHGLLVDA
                     QGQVWYTGNGNGTIGRLDPKTGKVTEYKAPSGGDPHTLITDGKGTIWFTVQNGGRVGR
                     LDTATGKITEYESDGRPYGIALDRQGNVWFCRMGADKLGKIDPRSGKISEFDTGAGSA
                     PRRMATAPDGTLWVTLFGNGKLLHFDPVQGKPIKEYALPAGHSGGPYAVTIDGSGLVW
                     ANEINTDTVVRLDPKTGQMRTIELPSRNTGIRKMIVDAGGRLWYMGSHNGRLGVVE"
     gene            complement(4668..>5190)
                     /locus_tag="VD791_03235"
     CDS             complement(4668..>5190)
                     /locus_tag="VD791_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXV07014.1"
                     /translation="SRMMLAIARIPQPVIARVQGIATAAGCQLVAQCDLAVASENARF
                     AVSGINVGLFCSTPSVPLGRNVLRKQAMEMLLTGDFIDAATAQRYGLVNRVTPPERLD
                     AEVAALAQRIVEKSAVAVATGKRMFYKHLEMGLEGAYQYAAETMACNMMTEDVGEGID
                     AFMQKRPAKWKHK"
BASE COUNT          768 a         1826 c         1805 g          791 t
ORIGIN      
        1 ggcacgcgca cgatctcttc gccgcgcagc acgtacaccg ccgcctcccc cggccgccgc
       61 gcttcgcgac gccgcgcgtc gctgttgccg gcgacggccg cgccggtgcc ggtcttggct
      121 tcgctcgggc ggaagcgcag cgcggcgttg ggcaccagga ccgcatgctc ggcccggtcg
      181 gtgacgatgg cgagataggc cgtcatgccc ggcatcagcg ccaggtcggg attggcgact
      241 tcgaccacga cgttgtaggt caccacgttc tgctgcgtga gcggcgacag ccggatctga
      301 tgcacccgcc cctcgaagcg gcgctcgggg taggcgtcca cggtgaagcg caccggctgg
      361 ccgaccgcga ccttgccgat gtcggcctcg gcgacgttgg agtcgatctg catgcgcgac
      421 agatcgcgtg cgatcttgaa cagggtgggc gtctggaagc tcgccgccac cgtctgaccc
      481 acgtccacct cgcgcgacac caccacgccc gccaccggcg agcggatcac cgcgtagccc
      541 agattggcgc ggtcgcgcgc cagcttgccc tgtgcggtgc gcacttgcgc ctgcgccgcc
      601 tcgcgcgcct gcacggcctt ctccagctcc tggcgcgaga cgtactcctg cgcgtgcagc
      661 tcgcgcatgc gcgtctcctc ctggcgggtc agccgcagcg tggcctcggc gctggccagc
      721 tcccccgagc tctgctcgac cgtcgcgcgc agcgtggtgg gatcgagctc cagcagcacc
      781 tgcccggcct tcacccggtc gttgaagtcg gcgtggatgc gcagcacggt gccggacacc
      841 tgggtgccga cgttcaccag tccgaccggg ttgacggtcc cgttcgcggt gacggtctgc
      901 gccagatcgc cggtggtcac cgcctccagc tgatagcgct cctcgagcgg ctgctcgcgc
      961 catttctgcc acgcgagcac ggcggcgagg ccgatcgcca gcagcagcaa cccgacggcg
     1021 gcacgcagga gatggggccg caacctggtc atcgcaggag cctggtcatc gcggcaaggg
     1081 ggcgctcgac tgggacaatt gcgccaccaa tgcaggatcg agcacgccca cggcgcgcgc
     1141 cagcgccgcc ttggccacgt tccagcgcag gcgcgcctcg atgcgttgca ccagcgcggc
     1201 ggacagggcg ctctgggcgt tgagcagatc gatgacggtg cccacgccgg cacgatagcg
     1261 cgccagggcc acgttgtagg attcctgcgc gctctcgaca aggtcgttcg cggtggcgag
     1321 cgactggccc tcggtgcgca ggttctggta cgcgagccac acctcgagcg cgacgtcgtc
     1381 ggccagccgg tcgcgcgtgg ccgcctgctc cagcacctgt tcgcgcgcct gctggatgcg
     1441 gtaagtgttg aagtagccgg tgaagatcgg gatgctgacc gacaacccga tcgcgcccga
     1501 caccgggtcc gggcccgggt tgctcaaggt ggcgcccgcc gaaccgaaca gcgacaccgt
     1561 gggcttcgcc gccgactcgc gcacgcgcac gttggattcg gcggcccgca cgcgcgcttc
     1621 cgccgccgcc agctccgggc gctgggcgcg cgcggccgcc agcagctcgc ccatggagcg
     1681 ctcgatcgcg cgctgcgctt cgaggtccgg cggcggtgca atgctgagcg gccggtccgg
     1741 cgatatgccg agcacattgg ccaggacgcc ttgcgagcgc gccgcttccc cctccgcctg
     1801 ggtgcggttg aacaccgcct gcgagtacgc cgtctgcgcc tgcagcacgt ccgcgcgcgt
     1861 ggcgctgccc gcgcggagcc gcgcccgcgc cgcttccagg ctctgcagcg ccgacttctc
     1921 ggcggccaca gtggcgtcca cggcttcgcg cgtcgcgaac agcaggtagt aggcggtgat
     1981 ggcatcgagc atcaccgtct gcagcgtcgc gttgtgcgtc cagttggcgg cagccagcga
     2041 ctcggccgcc agcgcgatcg cggcgtcgcg cccgccgaag tcgaacagca gccagctgaa
     2101 cccgacgctg gccggaagca gggtgaagcc ctgcgggtcg tcgatgttgc gcacttcccg
     2161 gcgttcgagc tcgatcaggg cgtccaccgt gggcagccgc gccgagcgcg ccacccccac
     2221 ctcggccgcc gcctgacggg cgatcgccca ggactggcgc gtgcgcgggt tgttgcacag
     2281 cgccaggtcg atcgcctcca ccagccgcaa cgtctccggc atggtcgccg cgtcggggca
     2341 gggcgccggc cccggcgggc ccgaccagaa gctgctcggc gcggcgggaa gctgcttgcg
     2401 ggtctcgaac gggtcggcgc cacgctgacc gagcgcggtg gcgggcgcca gggcgagcag
     2461 cagggcacag gctacccggc cgggtgcgaa gggaagtgga ggcatcttgg gcaggcgtcc
     2521 ggtagaaacc gccattaggc tgcgaccgtc gtcccgaaca atcgttgcat gcgcatggaa
     2581 tccggctggc ccccggggag agcgagctgg cgaggcgacc cggccgccag ggccatcaac
     2641 gcgccggcgg cggagccgtg cacagggcgt gctggagcgc gcgtgccgcg ctgcgcggcg
     2701 gtacaattcc tcttcgctcg accgggggag ggacagcacg tctcatgcca tcgccggccg
     2761 cactgagcgg acggcgcctg ccgcgcagga cgatcacccg actccccacg acaaccagga
     2821 gggacccatg gctttgcgaa tcggagacga agcacccaat ttcaccgcgc agacgacgga
     2881 aggcacgatc gacttccaca agtggatcgg cgacggctgg gccatcctgt tctcgcatcc
     2941 gaaggacttc accccggtgt gcaccaccga gctgggctac atggcgcggc tgaagccgca
     3001 attcgacaag cgcaactgca aggtcatcgg cctgagcatc gacccggtcg cggaccacga
     3061 gaagtgggcc agggacatcc aggagacgca gggctacgcg cccaattacc cgttgatcgg
     3121 cgacgccgac ctgaaggtgg cgaagctcta cgacatgatc cacccgaacg cgagcggcga
     3181 gcagaagcgc acggctgcgg acaacgccac catccgctcg gtgttcgtgg tcgggccgga
     3241 caagaagatc aagctgatcc tgacctaccc gatgagcacc gggcgcaact tcgacgaggt
     3301 gctgcgcgtg ctggactcga tgcagctcac cgccaagcac aaggtcgcca cgccggtgaa
     3361 ctggaagcag ggcgaggacg tgatcatcgt cccgtcggtg cccgacgagg aggcgaagaa
     3421 gcagtttccc ggcgggtgga aggcgcccaa gccgtacctg cgcatcgtgc cgcagctgaa
     3481 gtaggcgtgc cggagcgcgg agcaggggcc ccgcttgggg ggatgcgacc gcggaggcgc
     3541 gccgcgcgcg acaccgcaga acggtgcgcc ctgacttggt tcggagcgcg cgggctgtga
     3601 cggcatcgca tcgcttccgc gctgcgaagc cgcatcagcc tccgccgcaa tggccgacgc
     3661 aggtttggat ggtcactcca ccactccgag tcggccattg tgtgaaccca tgtaccacag
     3721 gcggccgccg gcatcgacga tcatcttgcg gatgccggtg ttgcgcgaag gcagctcgat
     3781 cgtgcgcatc tgcccggtct tcgggtccag gcgcaccacg gtgtcggtgt tgatctcgtt
     3841 ggcccagacc aggcccgagc cgtcgatggt caccgcgtaa ggcccgccac tgtggccggc
     3901 cggcagcgcg tattccttga tcggcttgcc ttggaccggg tcgaaatgca gcagcttgcc
     3961 gttgccgaac agcgtgaccc acagcgtgcc gtccggggcg gtggccatgc gccgcggcgc
     4021 gctgcccgcg ccggtgtcga actcgctgat cttgccgctg cgcggatcga tcttgccgag
     4081 cttgtccgcg cccatgcggc agaaccagac gttgccctgc cggtccagcg cgatgccgta
     4141 cggccggccg tcgctctcgt attccgtgat cttgccggtg gcggtgtcca gccggcccac
     4201 gcgcccgccg ttctgcaccg tgaaccagat cgtgcccttg ccgtcggtga tcagggtgtg
     4261 cggatcgccg cccgacggcg ccttgtactc ggtgaccttg ccggtcttcg gatccagcct
     4321 gccgatggtg ccgttgccgt tgccggtgta ccagacctgg ccttgcgcat cgaccagcaa
     4381 cccatgcggc ttggctcccg gcggcagttc ccattccttg aaggtttcgg ttttcgggtc
     4441 gaagcgcgcg atcttgttac cgtacatcac ggcgatgtag atcgcaccgt ccggacccgg
     4501 cgcaggatcg cgcgcgaact gcggcgtggg cacgggccac tcgcgcgcat tgccggcgaa
     4561 ggccgcgttg ctgccgggcc gcacggtgta gtcggagccg ccggcaacgg cggcggccgg
     4621 taacaacaga gccagcagcg cgagcgcgcg cgggcgtttg cgatgcgtca cttgtgcttc
     4681 cattttgccg ggcgcttctg catgaaggcg tcgatgcctt cgcccacgtc ctcggtcatc
     4741 atgttgcagg ccatggtctc ggcggcgtac tggtaggcgc cttccagccc catctcgagg
     4801 tgtttgtaga acatgcgctt gccggtggcc accgcgaccg ccgatttctc cacgatgcgc
     4861 tgcgccaggg ccgcgacctc ggcgtcgagc cgctccggcg gcgtcacgcg gttcaccagg
     4921 ccgtaacgct gcgccgtggc ggcgtcgatg aaatcgcccg tgagcagcat ctccatcgcc
     4981 tgcttgcgca gcacgttacg cccgagtggt acgctcggag tggagcagaa cagcccgacg
     5041 ttgatgccgg agacggcgaa ccgtgcgttc tccgaggcca ccgccaggtc gcactgcgcc
     5101 accagctgac agcccgccgc ggtggcaatg ccctggacgc gcgcgatcac cggctgcggg
     5161 atgcgcgcga tggcgagcat catgcggctg
//
LOCUS       DASPGE010000101         4648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1552891, whole genome shotgun sequence.
ACCESSION   DASPGE010000101 DASPGE010000000
VERSION     DASPGE010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552891"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VD791_03240"
     CDS             <1..187
                     /locus_tag="VD791_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07015.1"
                     /translation="AAGLEAYRQRDWDEALALFRQTLTLFPGDGPARTMAERCEACRR
                     TPPPAQWDGVFEQTFKK"
     gene            333..1631
                     /locus_tag="VD791_03245"
     CDS             333..1631
                     /locus_tag="VD791_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV07016.1"
                     /translation="MARYPNVIASLIAAIGLCGFAAGARAAPPAGAPAELKIGVVAFL
                     SGPASGPFGIPSRNAAQLWIDKINSEGGIGGVKVVPVFTDEAGPADKVVTEFRRLALD
                     QKVSAVMGYISSANCLAVPPVADELKVLTVLADCGTDRVFEEGKHPYVFRTHAHTVID
                     GVGAARYVLAVNPKVKTIAGINQDYAWGRDSWEVFQRSIRKLKPDVQVVDALWPKIFA
                     GEYSTEISKLLVNKPDVVFTSFWGGDLVTFSGQAKARGLLEETMVVVNNTGADIKDLA
                     PGMLIGSRGPHGIVSPDLEKNPLRKQFIDLYRQKHGLAANNYIAHGAYHYLQALQGLR
                     AAYEKAIKAAGRWPTPEQVAAAFKRLEFDTPSGHIAMAIGDGHQAVEPAGYAVIKFDS
                     ALGVNTFADIRMFSARCVNPPDGVKSLDWIDRGFPGAQCP"
     gene            1728..2624
                     /locus_tag="VD791_03250"
     CDS             1728..2624
                     /locus_tag="VD791_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV07017.1"
                     /translation="MRLLTVIALDGLAYASWLFLVSAGLTFTYGVLRILNLAHGNLYA
                     LGAYLGATLVLRYATAGSTVALGYGLLLLGAVAIGIVAGPLIERLFLRRVYAKDEEVQ
                     LLLTFSISLILEDVVKLTWGVSPIFADAPYTALGRVSLGGVNYAWYPFLLTAIALAVG
                     TLLWLVVTRTRLGKVLQAVITDREVSLTLGINVPRVYTWAFTVGAVLAALGGAYTAPM
                     FSLVPGVGAEVLVTSFAVVAIGGLGSIGGAALGALIVGMARSAAVYLFPELDLFAIYF
                     MMALVLLFRPQGLFGEIEMRRI"
     gene            2629..3576
                     /locus_tag="VD791_03255"
     CDS             2629..3576
                     /locus_tag="VD791_03255"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV07018.1"
                     /translation="MSPRVRAAAVPGTLAVLALLPFVLSGWVLFLLTVAGAKALAVLG
                     VVLLLRGKLLTFGHALYYAAGAYAAGMAVKYLNVREALVLLALALLAGALLSALLGLL
                     VARYRAVYFALLNLAFSMVLYGILLKFYSVTGGTDGLGIAEPTLAGWRLGGESLRLAM
                     YELTLILVALLVYLAHRFSVSPVGYALRAIRDNEVRIEYVGASVWHTVYVSYVIAGAL
                     AGVAGALIGISVGHITPDLAFWSQSGEFVFVAILGGIGSVLAPVVGSILFEFVRNYAF
                     EVSPYTWQMTLGVVLLLIIFFLPGGVWSLAHLATRRSTR"
     gene            3573..4316
                     /locus_tag="VD791_03260"
     CDS             3573..4316
                     /locus_tag="VD791_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV07019.1"
                     /translation="MSALLETVGLTRFFGAVRAAEDLSVQVAEGEMLGIIGSNGSGKT
                     TFLNLATGYLKPERGRILLAGRETTGLPPRTITRMGVARSFQIPQLYLGLTVIENMLL
                     PLAARAGAIGVWAPLHQAQWRAEGLRILGQFALEQYAERSVRELPEGVRKLLDIAIAV
                     ALRPRLLLMDEPTAGVSSEDKFEVMDTLVAVLRGSGITTIFVEHDMDVVRRYSQRVLV
                     FDQGLVIADGEPQNVLSDPLVRTAVLGHV"
     gene            4371..>4648
                     /locus_tag="VD791_03265"
     CDS             4371..>4648
                     /locus_tag="VD791_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV07020.1"
                     /translation="MNQPLPSTNLLSLDNVRVAIQGAEILRGVSLSVARGGLVALVGR
                     NGAGKTTTLRSVMGLLPLAGGRITVDGEDLAQVPPFRRAQLGIGYVPE"
BASE COUNT          648 a         1572 c         1605 g          823 t
ORIGIN      
        1 cgccgccggg cttgaggcgt atcgccagcg ggactgggac gaggccctcg cgctgttcag
       61 acaaaccctg acgctctttc cgggagatgg gccggctcgg acgatggcgg agcgctgcga
      121 agcctgccgg agaaccccgc ccccggcaca gtgggatggc gtgttcgagc agaccttcaa
      181 gaagtaaaaa gcttccacgg tttccaccgg cccggcacac cgccgtgctc ttcgcgcctg
      241 gcccgggggc gcgcttgacg cggatggacc gagccgatag atttgaaccc cggcatacgc
      301 cgcacgggtt gtcgctcgag ggaggacgcg ccatggctcg ctatccgaat gtcatcgctt
      361 cgctgatcgc cgcgatcggt ctctgcggtt tcgccgcggg cgcgcgcgcg gcaccaccgg
      421 cgggcgcacc ggcggaattg aagatcggcg tggtcgcgtt cctgtcgggg ccggcttccg
      481 gcccgttcgg catcccgtcg cgcaacgccg cccagctgtg gatcgacaag atcaacagcg
      541 agggcggcat cggcggcgtc aaggtggtgc ccgtgttcac cgacgaggcc gggccggccg
      601 acaaggtggt gaccgaattc cggcggctgg cgctcgacca gaaggtgagc gcggtgatgg
      661 gctacatctc cagcgccaac tgcctggcgg taccccccgt ggccgacgag ctgaaagtgc
      721 tcaccgtgct ggcggactgc ggcaccgacc gcgtgttcga ggaaggcaag catccttacg
      781 tgttccgcac ccacgcgcac acggtgatcg acggcgtggg tgcggcgcgc tacgtgctgg
      841 cggtgaaccc gaaggtgaag accatcgccg gcatcaacca ggactacgcc tgggggcgcg
      901 actcctggga agtgttccag cgcagcatcc gcaagctcaa gcccgacgtg caggtggtcg
      961 acgccctgtg gccgaagatc ttcgccggcg agtactcgac cgagatctcc aagctcctgg
     1021 tgaacaaacc cgatgtcgtc ttcaccagct tctggggcgg cgacctggtg accttctccg
     1081 gccaggccaa ggcgcgcggg ctgctcgagg agaccatggt cgtggtcaac aacaccggcg
     1141 ccgacatcaa ggacctggcg ccgggcatgc tgatcggttc gcgcgggccg cacggcatcg
     1201 tctcgcccga cctggagaag aatccgctgc gcaagcagtt catcgacctg taccggcaga
     1261 agcacgggct cgcagcgaac aactacatcg cacacggcgc ctaccactac ctgcaggcgc
     1321 tccagggctt gcgcgccgcc tacgagaagg cgatcaaggc ggccggccgc tggcccaccc
     1381 ccgagcaggt cgcggccgcc ttcaagcgcc tggagttcga cacgcccagc ggccacatcg
     1441 ccatggccat cggcgacggc catcaggccg tcgagccggc gggctacgcg gtgatcaagt
     1501 tcgactccgc gctgggcgtg aacaccttcg ccgacatccg catgttctcc gcccgctgcg
     1561 tcaatccgcc ggacggggtg aagagcctgg actggatcga tcgcggcttc cccggcgcgc
     1621 agtgtcctta ggcgccctta aggcgcgact taaaaagact accttcctcc cgtgaggagg
     1681 ggtcccgacg gtcgcggagt ccgataagcc ctcactaaac caccgccgtg cgcctgctga
     1741 cggtcatcgc gctggacggc ctcgcgtacg cgtcctggct gttcctcgtc tccgcggggc
     1801 tgacgttcac gtacggcgtg ctgcggatcc tcaacctggc ccacggcaat ctgtacgcgc
     1861 tgggcgcata tctcggcgcg acattggtgc tgcgctatgc gaccgccggc tccacggtag
     1921 cgctcgggta cggcctgctc ctgctcggcg cggtggcgat cgggatcgtg gccgggccgt
     1981 tgatcgagcg cctgttcctg cggcgcgtct acgccaagga tgaggaggtc cagctgctgc
     2041 tgacgttctc gatctccttg atcctcgaag acgtggtcaa gctcacctgg ggcgtgagcc
     2101 cgatcttcgc cgacgcgccg tacaccgcgc tggggcgcgt gagcctgggg ggcgtgaatt
     2161 acgcctggta cccgttcctg ctgacggcga tcgcgctggc cgtcggcacc ctgctctggc
     2221 tggtggtcac gcgcacgcgg ctgggcaagg tgctgcaagc ggtgatcacc gaccgcgagg
     2281 tcagcctgac gctgggcatc aacgtgccgc gcgtgtacac ctgggcgttc accgtcggcg
     2341 ccgtgctggc cgccctgggc ggggcgtaca ccgcaccgat gttctccctg gtgcccggcg
     2401 tgggggcgga ggtgctggtg acctccttcg ccgtcgtcgc catcggcggc ctgggcagca
     2461 tcggcggcgc ggcgctcggc gcgctcatcg tgggcatggc gcgctcggcc gcggtgtacc
     2521 tgttccccga gctggacctg ttcgcgatct acttcatgat ggcgctggtg ctcctgttcc
     2581 ggccgcaggg cctgttcggc gaaatcgaga tgcggaggat ctgagcgcat gtctccgcgc
     2641 gttcgcgccg ccgccgtccc cggcacgctc gccgtgctgg cgttgctgcc cttcgtgctg
     2701 tcgggttggg tgctgtttct gctcaccgtg gcgggggcca aggcgctggc ggtgctgggc
     2761 gtggtgttgc tgctgcgcgg caagctgctc accttcggcc atgcgctgta ttacgccgcc
     2821 ggcgcctatg ccgccgggat ggcggtgaag tatctcaacg tgcgcgaggc cctggtgctg
     2881 ctcgccctcg cgctgcttgc gggcgcgttg ctcagcgcgt tgctgggtct gctggtggcg
     2941 cgctaccggg cggtgtactt cgcgctgctc aatctcgcct tctcgatggt gctctacggc
     3001 atcctgctca agttctactc ggtgaccggc ggcaccgacg gtctgggcat cgccgagccc
     3061 acgctcgccg gctggcgcct gggcggcgag tccctgcgcc tcgccatgta cgagctgacg
     3121 ttgatcctgg tggcgctgct ggtctatctc gcccaccgct tttccgtctc gccggtcggc
     3181 tatgccctgc gcgcgatccg cgacaacgag gtgcgcatcg aatacgtcgg cgcgtcggta
     3241 tggcacacgg tgtacgtctc ctatgtcatc gccggcgcgc tggccggcgt cgcgggcgct
     3301 ttgatcggca tctcggtcgg gcacatcaca cccgatctcg cgttctggtc gcaatccggc
     3361 gagttcgtct tcgtcgcgat cctgggcggc atcggcagcg tgctcgcccc cgtggtcggc
     3421 tcgatcctgt tcgagttcgt gcgcaactac gctttcgagg tctcgcccta cacctggcag
     3481 atgacgctgg gggtggtgct gctgctgatc atcttcttcc tgcccggcgg ggtgtggagc
     3541 ctcgcgcatc ttgccacgcg gcggagcacc cgatgagcgc gctgctggaa accgtcggcc
     3601 tgacccggtt cttcggcgcg gtgcgcgccg cggaggactt gagcgtgcag gtagcagagg
     3661 gcgagatgct cggcatcatc ggctccaacg gctcgggcaa gaccacgttc ctgaatctcg
     3721 ccaccggcta cctcaagccc gaacgcggcc gcatcctgct cgccggcagg gaaaccaccg
     3781 ggctgccgcc gcgtaccatc acgcgcatgg gcgtggcccg ctccttccag attccacagc
     3841 tgtatctggg gctgacggtg atcgagaaca tgttgctccc gctcgcggcg cgcgccggcg
     3901 cgatcggcgt gtgggccccg ctgcaccagg cgcagtggcg tgccgagggc ctgcggatcc
     3961 tcgggcagtt cgccctcgag cagtacgcgg agcgttcggt gcgcgagctg ccggaaggcg
     4021 ttcgcaagct gctcgacatc gccatcgcgg tggcgctgcg tccgcggctg ctgctgatgg
     4081 acgagcccac ggcgggcgtc agcagcgagg acaagttcga agtgatggac accctggtcg
     4141 cggtgctgcg cggaagcggc atcaccacca tcttcgtcga gcacgacatg gacgtcgtgc
     4201 gccgctacag ccagcgcgtg ctggtgttcg accaggggct ggtgatcgcc gacggcgagc
     4261 cgcagaacgt gctctccgat cccttggtgc ggacggcggt gctcggtcat gtctagtgcg
     4321 ctgcaatgcc ccctctcccc ttgcgggaga gggtagggtg aggggtacga atgaatcagc
     4381 cgctaccgtc caccaacctg ctctctctgg acaacgtccg cgtcgcgatc cagggcgccg
     4441 agatcctgcg cggcgtttcg ctctcggttg cacgcggcgg cctggtggcg ctggtaggcc
     4501 gcaacggcgc gggcaagacc accacgctgc gcagcgtgat ggggctgctg ccgctcgccg
     4561 gcgggcgcat caccgtggat ggcgaggacc tggcgcaggt gccgccattc cggcgtgcgc
     4621 agctcggcat cggctatgtg cccgaaga
//
LOCUS       DASPGE010000102         7643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1202542, whole genome shotgun sequence.
ACCESSION   DASPGE010000102 DASPGE010000000
VERSION     DASPGE010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7643
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202542"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2174
                     /gene="infB"
                     /locus_tag="VD791_03270"
     CDS             <1..2174
                     /gene="infB"
                     /locus_tag="VD791_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011884588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HXV07021.1"
                     /translation="RQAAEAAAKAQRGRKRKAAEAAPPAEQAAAELASAPGATAAAAE
                     AKPAEAKPEVKQAAKTGAAGTLHKPAAKAGEGEKKVAKKATKDTWRDDAARRRTIKTR
                     GGESVGGWHARRDRHARGRPTTEAAGQHAFSLPMEPVVHDVLIPETITVANLAQKMSV
                     KAAEVIKALMKLGTMVTINQVLDQDTAMIVVEEMGHVAKRAKLDDPEAFLAETAQEAS
                     VPAEPRAPVVTVMGHVDHGKTSLLDHIRRTRVASGEAGGITQHIGAYHVETARGTITF
                     LDTPGHEAFTAMRARGAKVTDIVVLVVAADDGVMPQTVEAIHHAKAAKVPVLVAVNKI
                     DKPEAQPERIRQELVAHAVVPEDWGGDTIFVDVSAKTGANIDTLLESILLQAEVLELK
                     APKSTPGKGIIIESRLDKGRGPVATVLVQSGTLNRGDIVLAGAVFGRVRAMLDEAGRA
                     VQHAGPSIPVEIQGLSDVPIAGEEVMVLGDERKAREIALFRQGKFRDVKLAKQQAAKL
                     EGMFEQMGAGEHKALSLIIKADVQGSQEALVHALQKLATEEVKVNVVHSGVGAITESD
                     INLALASNAVIVGFNTRADATAKKLMAAHGVDVRYYNVIYDAVDEVKAALSGMLAPEK
                     KESVTGLVEVRQVFHISKVGTVAGCYVLEGVVRRSAMVRVLRDNIVIHAGELDSLKRF
                     KDDVREVKAGFECGLSVKSFSDLKVGDQLEVFEVVEVARTL"
     gene            2212..2589
                     /gene="rbfA"
                     /locus_tag="VD791_03275"
     CDS             2212..2589
                     /gene="rbfA"
                     /locus_tag="VD791_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006053158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HXV07022.1"
                     /translation="MPRDFSRASRLADQIQRDLSEVIRTELKDPRVGMVTITAVEVSR
                     DLSHARVFVTSLAGAQAMEESLGALQHAAGFLRSRLAHSLSLRTVPELQFAYDESIER
                     GARLSRLIDEANQPAAGSDPDPA"
     gene            2613..3530
                     /gene="truB"
                     /locus_tag="VD791_03280"
     CDS             2613..3530
                     /gene="truB"
                     /locus_tag="VD791_03280"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HXV07023.1"
                     /translation="MRKGTPLDGVLLLDKPAGVTSNAALQQAKRLLGAAKAGHTGTLD
                     PLATGLLPVCFGEATKFAGLHLEADKTYLATIQLGASTTTGDAEGEVVFRGDTGGLGR
                     ERVSVVLAQLTGAQEQVPPMYSALKREGRPLYAYARAGETVERSGRPVTIHELTAVRI
                     APEELELRLRVSKGTYVRAVAQEIGERLGCGAHLKTLRREASGGLSLADSITLERLSA
                     LPPAQRVAVLRPVDILVAPLPRGELDAPSAAALQQGRTVGTALALASGLVRLYDPQGA
                     FLGVGEMQGSGRVAPRRLLAARPGAGAAA"
     gene            3691..3960
                     /gene="rpsO"
                     /locus_tag="VD791_03285"
     CDS             3691..3960
                     /gene="rpsO"
                     /locus_tag="VD791_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HXV07024.1"
                     /translation="MAITTELKSRLVGEYQRAKGDTGSPEVQVALLTARINELTEHFK
                     THLKDFHSRRGLLQMVSRRRKLLDYLKRSDNERYRTVLDRLGLRK"
     gene            4040..6148
                     /gene="pnp"
                     /locus_tag="VD791_03290"
     CDS             4040..6148
                     /gene="pnp"
                     /locus_tag="VD791_03290"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HXV07025.1"
                     /translation="MDVNPIASVKKSIQYGRHTLTLETGEIARQAHGAVMVRLDDTCV
                     LVSVVGRKEVKAGQDFFPLTVDYMERTYAAGRIPGGFFKREGRPSEKETLTCRLIDRP
                     IRPLFPEGLFNEVQVVATVVSSDNEIDSDIPALIGASAALALSGIPFNGPIGAARVGY
                     ANGEYILNPTASELKESQLNLVVAGTQAAVLMVESEANELPEEVMLGAVVYGHEQMQA
                     VIDMVNDLADEAGKPLWDWTPPQKDEALVRKVAELAEAGLEEAYRTRQKQQRTDKLAA
                     LSAAVTEALITNTETPADPNLVNTLFSDLESKIVRSRILGGEPRIDGRDTRTIRPIAI
                     RTGVLPRAHGSALFTRGETQALVVATLGTARDEQIIDALQGEYSERFMLHYNMPPYAT
                     GETGRVGPPKRREVGHGRLAKRALVNMLPTPEQFGYSMRVVSEITESNGSSSMASVCG
                     GSLALMDAGVPMKAHVAGIAMGLIKEGNRFAVLSDILGDEDHLGDMDFKVAGTERGVT
                     ALQMDIKIQGITKEIMQVALNQAREGRMHILALMKQALEAPRPEISAYAPRMIVIKIK
                     PEKIRDVIGKGGAVIRAITEETGTTIDIAEDGTVTIACVSSDGGLAAKKRIEDITAEV
                     EVGRVYEGTVLKLLDFGAIVSVLPGKDGLLHISQIAEERVKNVADHLKEGQSVRVKVL
                     EADDKGRLRLSMKAVAAETH"
     gene            6210..>7643
                     /locus_tag="VD791_03295"
     CDS             6210..>7643
                     /locus_tag="VD791_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HXV07026.1"
                     /translation="MSNDVRTATGGRILVDALGAHGVDLAFCVPGESYLAVLDALYDV
                     RDALRLVVCRQEGGAAFMAEAYGKLSGRPGICFVTRGPGATNASIGVHTAYQDSTPMI
                     LFIGQVGSDVVEREAFQEVDYRRMFGPMAKWVAQIDRADRVAEYVSHAFHLAVSGRAG
                     PVVLALPEDMLVAQAPVVPLVPYRRVKPHPGPAELERMAQMLREAQRPLLMLGGGDWS
                     QAACDDARAFVEAWNLPTACTFRRQDLLDNRHPNYAGEIGIVTGAELKKRVREADLVL
                     AVGPRLGEMTTGGYTLFDIPAPRQRIVHVHPGSEELGRVYQAELMINSGMAEFCAAAR
                     RLPAIAKPGWAQWTAALRAEYLKTLEPVPAPGQINMSEVVAWLRGRLPEGSIVTNGAG
                     NFSGWPQRFHQYTGFRTQLAPTNGAMGYGVPAAIAAKLVHPARTVVCFAGDGDFLMTG
                     QELATAVQYSAPVVFLVLNNSMYGTIRM"
BASE COUNT         1297 a         2585 c         2715 g         1046 t
ORIGIN      
        1 cgcgccaggc cgccgaggcc gcggccaagg cccagcgcgg caggaagcgc aaggcggccg
       61 aagcggcgcc gcccgcggag caggccgccg ccgagctcgc ctcggccccg ggagcaaccg
      121 ctgccgcggc cgaggcgaaa ccggcggagg cgaagccgga ggtcaagcag gcggcgaaga
      181 cgggcgccgc gggcactttg cacaagcctg ccgccaaggc gggcgaaggc gagaagaaag
      241 tcgccaagaa ggcgaccaag gacacttggc gcgacgatgc ggccaggcgg cgcaccatca
      301 agacccgcgg cggcgagagc gtgggcggat ggcacgcgcg ccgcgaccgc cacgcgcgcg
      361 gccggccgac gacggaagcc gccggccagc acgccttttc gctgcccatg gagccggtgg
      421 tgcacgacgt cctgatcccg gaaacgatca cggtcgcgaa cctggcgcag aaaatgtcgg
      481 tgaaggcggc cgaggtgatc aaggccctga tgaaactcgg caccatggtc accatcaatc
      541 aagtgctcga ccaggacacg gcgatgatcg tggtcgagga aatgggccat gtggccaagc
      601 gcgccaagct cgacgacccg gaagccttcc tcgccgagac cgcgcaggag gcctccgtgc
      661 ccgcggaacc gcgagcgccg gtggtgaccg tgatgggaca cgtcgaccac ggcaagacgt
      721 cgctgctcga ccatatccgc cgcacgcgcg tggccagcgg cgaggccggc ggcatcaccc
      781 agcacatcgg cgcctatcac gtcgagaccg cgcgcggcac catcaccttc ctcgacacgc
      841 ccggccatga ggcctttacc gccatgcgcg cgcgtggcgc gaaggtgacc gacatcgtcg
      901 tgttggtggt ggccgccgac gacggcgtca tgccgcaaac ggtggaggcg atccaccacg
      961 ccaaggccgc caaggtgccg gtgctggtgg cggtgaacaa gatcgacaag cccgaggcgc
     1021 aacccgagcg catccgccag gagttggtgg cgcacgcggt ggttccggaa gactggggcg
     1081 gcgacaccat tttcgtggat gtttcggcca agaccggcgc caacatcgac acgctgctcg
     1141 aatccatcct tctgcaggcc gaggtgctgg agctgaaagc gccgaagagc acgccgggga
     1201 aaggcatcat catcgagtcg cgcctggaca aggggcgcgg accggtcgcc acggtgctgg
     1261 tgcagtcggg tacgctcaac cgtggggaca tcgtgctcgc cggcgcggta ttcgggcgcg
     1321 tgcgcgcgat gctggacgag gctggacgcg cggtgcagca cgccggtccc tccattccgg
     1381 tggagatcca ggggttgtcc gacgtgccca tcgccggcga ggaggtgatg gtgctgggcg
     1441 acgagcgcaa ggcgcgcgag atcgccttgt tccgccaggg caagttccgc gacgtcaagc
     1501 tcgccaagca gcaggcagcg aagctcgagg gcatgttcga gcagatgggc gcgggcgagc
     1561 acaaggccct gtcgctgatc atcaaggccg acgtgcaggg ctcccaggag gccctggtgc
     1621 acgcgctgca gaagctcgcc accgaggagg tgaaggtgaa cgtcgtgcac agcggcgtgg
     1681 gcgccatcac cgagtcggac atcaacctcg cgctcgcctc caacgccgtg atcgtcggct
     1741 tcaacacccg ggccgacgcc accgccaaga agctgatggc ggcgcatggc gtggatgtgc
     1801 gctactacaa cgtgatctac gacgccgtgg acgaggtgaa ggcggcgctg tccgggatgc
     1861 tggcgcccga gaagaaggaa agcgtcaccg ggctggtgga ggtgcggcag gtgttccaca
     1921 tctccaaggt cggcacggtg gcgggctgct acgtgctgga gggcgtggtc cgacgcagcg
     1981 ccatggtgcg cgtgctgcgc gacaacatcg tgatccacgc cggcgagctg gattcgctca
     2041 agcgcttcaa ggacgacgtg cgcgaggtga aagccggctt cgagtgcggc ctgtccgtga
     2101 agagcttctc cgacttgaag gtgggcgacc agctcgaggt gttcgaagtc gtggaggtgg
     2161 cgcgcaccct gtagcgcgac tggcgcccct cccgcagccg cgcgccgtcc gatgccccgc
     2221 gacttctccc gcgcttcgcg cctcgccgat cagatccagc gcgacctgtc ggaggtgatc
     2281 cgcaccgagc tcaaggaccc gcgggtgggc atggtcacca tcaccgccgt ggaagtgagc
     2341 cgcgacctgt cgcacgcgcg cgtgttcgtg acctcgctgg ccggcgcgca ggcgatggag
     2401 gagagtctgg gcgccctgca gcacgccgcc ggcttcctgc gcagccgcct cgcgcacagc
     2461 ctgagtctgc gcaccgtgcc ggagctccag ttcgcctacg acgagtcgat cgagcgcggt
     2521 gcgcggctgt cgcgcctgat cgacgaggcg aaccagcccg ccgccggctc cgaccccgat
     2581 ccggcctgag acgacacccc cggtggtcgg cgatgcgcaa ggggactccg ctggacggcg
     2641 tgctgctgct cgacaagccg gccggggtca cctccaacgc cgccctgcag caggccaaac
     2701 gtctgctcgg ggccgccaag gccggccata ccggcacgct cgatccgctt gcgaccgggt
     2761 tgctgcccgt gtgcttcggg gaggcgacca agttcgccgg cctgcacctc gaggcggaca
     2821 agacctacct cgccacgatc cagctcggcg cgagcaccac caccggggac gccgagggcg
     2881 aagtcgtctt ccgtggcgat accgggggcc tcggccggga gcgggtctcg gtcgtcttgg
     2941 cgcagctcac cggcgcccag gaacaggtgc cgccgatgta cagcgccctg aagcgcgaag
     3001 gccggcctct gtacgcttac gcgcgggcgg gcgagaccgt cgagcgcagc ggccgcccgg
     3061 tcaccatcca tgagctgacc gccgtacgca tcgcgccgga ggagctggag ctgcgcctgc
     3121 gcgtgagcaa gggcacctac gtgcgggcgg tcgcccagga gatcggcgag cgcctggggt
     3181 gcggcgctca cctcaagacg ttgcggcgcg aggcgagcgg cggcctgagc ctggccgact
     3241 cgatcaccct ggagcgcctc tccgccctgc cgccggcgca gcgcgtggcg gtcctgcggc
     3301 cggtggatat cctggtggcg ccgcttcccc ggggcgagct ggacgcgccg tcggcggcgg
     3361 cgctgcagca gggccgaacc gtggggacgg cgcttgcgct cgcatccggg ctggtgcggc
     3421 tgtacgaccc gcagggggcc ttcctcggcg tcggggagat gcaggggagc ggtcgggtag
     3481 cgccgcgcag gctgctggcg gcccggccgg gcgccggggc cgccgcttga agccggcggt
     3541 cgcccccggg agcctcctcg ggtcaagcct ttgcggaact tgcggcgaaa aagccggatc
     3601 cgggctagaa tgcgcgtttt ttggagacaa ccccggtttt cttggagacc gccccgtttt
     3661 tctgtttttc ttggagacag acaagtaacg atggccatca cgaccgagct gaaatcgcgg
     3721 ctggtaggcg aataccagcg cgccaagggc gacaccggct cgcccgaggt gcaggttgcg
     3781 ctgctgaccg cgcgcatcaa cgagctgacc gagcacttca agacgcacct caaggatttc
     3841 cattcgcggc gcgggctgct gcagatggtg agccgccgcc gcaagctgct cgactacctc
     3901 aagcgctcgg acaacgagcg ctatcgcacg gtgctggacc ggctcggtct gcgcaagtga
     3961 agcggcacgc cgcatccgct ggcgtcccga tcaaccctcg ctgcgggcgc gctgcgcgcg
     4021 ccctcgaagc ctccctccta tggatgtgaa tccgatcgct tccgtcaaga aaagcattca
     4081 atacggccgc cacacgctca ccctcgagac cggcgagatc gcccgccagg cgcacggcgc
     4141 ggtcatggtt cgcctggacg acacctgcgt gctggtcagc gtcgtgggca gaaaggaagt
     4201 caaggccggc caggacttct tcccgctcac cgtggactac atggagcgca cctacgccgc
     4261 cggacgcatc ccgggcggct tcttcaagcg cgaggggcgc ccttcggaga aagaaaccct
     4321 cacctgtcgc ctgatcgatc ggccgatccg cccgctgttc ccggaggggt tgttcaacga
     4381 ggtgcaggtg gtggccacgg tggtctcgtc cgacaacgag atcgactccg acatcccggc
     4441 gctgatcggc gcatcggccg cgctggcgct gtcgggcatt cccttcaacg gcccgatcgg
     4501 cgcggcgcgg gtgggctacg cgaacggcga gtacatcctc aacccgaccg ccagcgagct
     4561 gaaggaatcc cagctcaacc tggtggtggc cggcacgcag gccgcggtgc tgatggtcga
     4621 gtccgaggcc aacgagctgc cggaggaagt gatgctcggc gcggtggtgt acgggcacga
     4681 gcagatgcag gcggtgatcg acatggtcaa cgaccttgcc gacgaggccg gcaagccgct
     4741 gtgggactgg accccgccgc agaaggacga agcgctggtg cgcaaggtcg cggaactggc
     4801 cgaggccggc ctggaggagg cctaccgcac gcgccagaag cagcagcgca ccgacaagct
     4861 ggctgcgctg agcgccgccg tgaccgaggc gctgatcacc aacaccgaga cgccggccga
     4921 cccgaacctg gtgaacacgc tgttcagcga cctggagtcg aagatcgtgc gcagccgcat
     4981 cctgggcggc gagccgcgga tcgacgggcg cgacacgcgc accatccgtc ccatcgctat
     5041 ccgcaccggc gtgctgccgc gcgcgcacgg ctcggcgctg ttcacccgcg gcgaaacgca
     5101 ggcgctggta gttgccaccc tgggcaccgc gcgcgacgag cagatcatcg acgcactgca
     5161 gggcgaatac agcgagcgct tcatgctgca ctacaacatg ccgccctacg ccaccggcga
     5221 gaccggccgg gtcggcccgc ccaagcgccg cgaggtcggc cacgggcggc tggccaagcg
     5281 cgcgctggtg aacatgctgc ccacgccgga gcagttcggc tactcgatgc gcgtggtgtc
     5341 cgagatcacc gaatccaacg gctccagctc gatggcctcg gtgtgcggcg ggtcgctcgc
     5401 gctgatggac gccggcgtgc cgatgaaggc gcacgtggcg ggcatcgcca tgggcttgat
     5461 caaggaaggc aaccgcttcg cggtgctgtc cgacatcctg ggcgacgagg accacctggg
     5521 cgacatggac ttcaaggtgg cgggcaccga gcgcggggtc accgcgctgc agatggacat
     5581 caagatccag ggcatcacca aggagatcat gcaggtggcg ctcaaccagg cgcgcgaggg
     5641 ccgcatgcac atcctcgccc tgatgaagca ggcgctggag gcgccgcggc cggagatctc
     5701 ggcctacgcg ccgcgcatga tcgtgatcaa gatcaagccg gagaagatcc gcgacgtgat
     5761 cggcaagggc ggcgccgtga tccgcgccat caccgaggag accggcacca ccatcgacat
     5821 cgccgaggac ggcaccgtga ccatcgcctg cgtcagctcc gacggcggac tggccgcgaa
     5881 gaagcgcatc gaagacatca ccgccgaggt cgaggtgggg cgcgtctacg aaggcacggt
     5941 gctcaagctg ctcgacttcg gcgccatcgt cagcgtgctg cccggcaagg acgggctgct
     6001 gcacatctcg cagatcgccg aggagcgggt caagaacgtg gccgaccacc tcaaagaagg
     6061 ccagtcggtg cgcgtcaagg tgctcgaggc cgacgacaag gggcgactac ggctgtcgat
     6121 gaaggcggtc gccgccgaaa cgcactagcg cgctctatcc ccggcctccc gcgaggccgg
     6181 cgcccagcag tcatcggagg cgcagcggca tgagcaacga cgttcgcacc gcgaccggcg
     6241 gcaggatcct ggtggatgcg ctgggcgcgc acggcgtcga tctcgccttc tgcgtgccgg
     6301 gcgagagcta cctggcggtg ctggacgcgc tgtacgacgt gcgcgacgcg ctacgcctgg
     6361 tggtttgccg gcaggaaggc ggcgcggcct tcatggcgga agcctacggc aagctcagcg
     6421 gccgccccgg catctgtttc gtcacccgcg gaccgggcgc caccaacgcc agcatcggcg
     6481 ttcacaccgc gtatcaggac tcgacaccga tgatcctgtt catcggccag gtgggcagcg
     6541 acgtcgtcga gcgcgaagcc ttccaggagg tcgattaccg gcgcatgttc ggaccgatgg
     6601 ccaagtgggt ggcgcagatc gaccgtgccg accgcgtcgc cgagtacgtg agccacgcgt
     6661 tccatctggc ggtctccgga cgcgccggcc cggtggtgct ggctcttccg gaggacatgc
     6721 tggtggcgca ggccccggta gtgccgctgg ttccgtaccg cagagtgaag ccgcacccgg
     6781 gcccagccga actcgaacgc atggcccaga tgctgcgcga agcgcagcgg ccgctgttga
     6841 tgctcggtgg cggcgactgg agccaggcgg cctgcgacga cgcgcgcgcg ttcgtcgagg
     6901 cctggaacct acccaccgcc tgcaccttcc gacgtcagga cctgctggac aaccgccacc
     6961 cgaactacgc cggcgagatc ggcatcgtca ccggcgccga gctgaagaag cgcgtgcgcg
     7021 aagccgacct cgttcttgcg gtcggtccgc ggctcggcga gatgaccacc ggcggctaca
     7081 cgctgttcga catccccgcg ccgcggcagc gcatcgtgca cgtgcacccg ggcagcgagg
     7141 agttggggcg cgtgtaccag gccgagctga tgatcaactc gggcatggcc gaattctgcg
     7201 ccgcggcgcg acgcctgccg gcgatcgcga agccggggtg ggcgcagtgg acggcggcgc
     7261 tgcgcgcgga atacctgaag acgctcgagc cggtgcccgc gccgggccag atcaacatga
     7321 gcgaagtggt ggcatggctg cgcggccgtc tgccggaagg cagcatcgtc accaacggtg
     7381 cgggcaactt ctccggctgg ccgcagcgct tccatcaata caccggcttc cgcacgcagc
     7441 tcgcgccgac caacggcgcg atgggttacg gcgtgccggc tgcgatcgcg gccaaactgg
     7501 tgcaccccgc gcgcaccgtg gtgtgcttcg ccggcgacgg cgatttcctg atgacgggcc
     7561 aggagctggc caccgccgtt caatactcgg cgccggtggt gttcctggtg ctgaacaaca
     7621 gcatgtacgg caccatccgc atg
//
LOCUS       DASPGE010000103         5833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1318682, whole genome shotgun sequence.
ACCESSION   DASPGE010000103 DASPGE010000000
VERSION     DASPGE010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318682"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="VD791_03300"
     CDS             complement(<1..1468)
                     /locus_tag="VD791_03300"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HXV07027.1"
                     /translation="MYLYDPIDQRLVDERVAQYRDQTRRFLAGRLSEDEFRPLRLRNG
                     LYIQKHAPMLRVAIPYGLLSTAQLRKLAFIARTYDRGYGHFSTRQNIQFNWPRLEEVP
                     DILAHLAAVQMHAIQTSGNCVRNITTDHFAGVAPDEIIDPLVWVEVMRQWSTLHPEFG
                     FLPRKFKIAMNGSVEDRAATLVHDIGLHAVRDAVGEVGFRVIVGGGLGRTPIIGHVIR
                     EFLPWRHLLTYLDAILRVYNRYGRRDNLYKARIKILVKDRTPAGFAAEVEAEWAHLKD
                     GPGTLAAEEIERIASRFTRPAYLRLPDTDAGYESRLKAEPAFARWVSRNVHPHKVPGY
                     AAVTLSLKKTGVPPGDATDVQMDAVADLADRYAFGEVRVTHEQNLVFADVPKRDLHAL
                     WLELKALGLATPNIGLLTNIIACPGGDFCSLANAVSIPVAEAIQQRFDDLDYLYDIGE
                     LDLNISGCMNSCGHHHVGHIGILGVDKNGEEWYQISIGG"
     gene            complement(1850..2821)
                     /locus_tag="VD791_03305"
     CDS             complement(1850..2821)
                     /locus_tag="VD791_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HXV07028.1"
                     /translation="MNLQQLRYVREAVRQGLNLTEAANALYTSQPGISKQIRELEDEL
                     GIEIFVRRGKRLVALTEPGQLVVRVIDRLLDEADNLRQVGKEFADQASGALTIATTST
                     QARYSLPVVVQRFRIQYPNVHLSLLQGTPTQIADMVCEGRADIAIATEALDHYPDLLA
                     LPGYTWHHCAIVPRGHALSKVTRLTLDELAKYPLITYSPAFSRRTHIDEAFAAKGLKP
                     DIVLTALDSDVIKTYTELGLGVGIVAAMAVDRKRDPGLSAIDAGHLFGTNTTRVAVKR
                     TAYLRGYTYAFIHAFAPHLTRAVVDRARQASAAGAEGTKPTRRPPRK"
     gene            complement(2883..3995)
                     /locus_tag="VD791_03310"
     CDS             complement(2883..3995)
                     /locus_tag="VD791_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HXV07029.1"
                     /translation="MSIEVRNLVKRFGAFTALDDVTLSFPSGQLVALLGPSGCGKTTL
                     LRVIAGLETPDSGAVLFHGEDASWQDVRERQVGFVFQHYALFRHMSVFENVAFGLRVK
                     RRAERPSNEQIRRKVLDLLKLVQLDWLADRYPHQLSGGQRQRIALARALAVEPKVLLL
                     DEPFGALDAKVRKELRRWLRRLHDELHITSIFVTHDQEEALEVADQVVLMNKGKVEQI
                     GTPDEVYERPASPFVYGFLGTVNVFHGRASNGRFELGTVSVEAPEHRDARNAPAVAYA
                     RPHEIEIERFSPGLDGLPVQLSRMLVVGPTARLELEREDSNEILEVEIPAPRARGLNL
                     KVGETLLIRPRRMQVFLDRSPVEPSILHGTSRAVIS"
     gene            complement(4030..4917)
                     /gene="cysW"
                     /locus_tag="VD791_03315"
     CDS             complement(4030..4917)
                     /gene="cysW"
                     /locus_tag="VD791_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HXV07030.1"
                     /translation="MLPLAAPAAARNRATAEPAYVRWLLIGLALAFLGVFLLVPLAAV
                     FTEATRKGVETYFAALTEPDAIAAIRLTLLVAAIAVPANLVFGVAAAWAIAKFDFRGK
                     SVLITFIDLPFAVSPVIAGLIYVLLFGAQGWLGPWLQAHDIRIVFAVPGIVLATIFVT
                     FPFVARELIPLMQAQGTEEEEAARVLGASGWQMFWRVTLPNIKWGLLYGVILCNARAM
                     GEFGAVSVVSGHVRGVTNTTPLHVEILYNEYNFAAAFAVASLLASLALVTLLLKSLVE
                     WRARRELALAAELSSERPA"
     gene            complement(4926..5801)
                     /gene="cysT"
                     /locus_tag="VD791_03320"
     CDS             complement(4926..5801)
                     /gene="cysT"
                     /locus_tag="VD791_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006580064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HXV07031.1"
                     /translation="MTTASTSIAALPGGLSRRPNVLPGFGLALGYTVLYLSLIVLIPL
                     SATFFKSAGLGWDAFWTTVTAPRVLASLRLSFGASLAAALINAVFGLLVAWVLVRYRF
                     FGRRVCDALVDLPFALPTAVAGIALTAVYAGNGWLGQYLEPLGIKVAFTPLGVIVALT
                     FIGLPFVVRTVQPVLEDLEPELEEAAATLGANRLQTFQRIILPAVWPALLTGFTLAFA
                     RAVGEYGSVIFIAGNMPMVSEITPLLIIIKLEQYDYAGATSVAVLMLVISFALLLLIN
                     LLQWWSGRRMGQTRG"
BASE COUNT          977 a         1942 c         2037 g          877 t
ORIGIN      
        1 ggccgccgat cgagatctgg taccactcct cgccgttctt gtccacgccg aggatgccga
       61 tgtgaccgac atgatggtgg ccgcaggagt tcatgcagcc cgagatgttg agatccagct
      121 cgccgatgtc gtacagataa tccaagtcgt cgaagcgctg ctggatcgcc tcggccaccg
      181 ggatggagac ggcgttggcg agcgagcaga aatccccgcc cgggcaggcg atgatgttgg
      241 tgagcaggcc gatgttcggg gtcgccaggc cgagcgcctt cagctcgagc cacagcgcat
      301 gcaggtcgcg cttgggcacg tccgcgaaca ccaggttctg ctcgtgcgtg acgcggacct
      361 cgccgaaggc gtagcggtcc gcgagatcgg cgacggcgtc catctgcacg tcggtcgcgt
      421 ccccgggcgg cacgccggtc ttcttcagcg acagcgtcac ggccgcatag ccgggcacct
      481 tgtgcggatg cacgttgcgc gacacccagc gggcgaaggc cggttcggct ttcagacgcg
      541 attcgtagcc ggcgtcggtg tcgggcaggc gcagataggc cggacgggtg aagcgcgagg
      601 cgatgcgctc gatctcctcg gcggccagcg tccccggacc gtccttcagg tgcgcccact
      661 ccgcctcgac ctcggcggcg aagccggccg gagtgcggtc cttcaccagg atcttgatgc
      721 gggccttgta caggttgtcg cgacgcccgt agcggttgta cacgcgcagg atcgcgtcca
      781 ggtaggtcaa cagatggcgc cacggcagga actcgcggat cacgtggccg atgatgggcg
      841 tgcggcccag gccgccgccg acgatcacgc ggaagcccac ctcgccgacg gcgtcacgca
      901 ccgcgtgcag accgatgtcg tgcaccagcg tcgccgcgcg gtcctcgacc gagccgttca
      961 tggcgatctt gaacttgcgc ggcaggaaac cgaactcggg atgcagcgtg gaccactggc
     1021 gcatcacctc gacccacacc agcggatcga tgatctcgtc cggggccacc ccggcgaagt
     1081 ggtcggtggt gatgttgcgc acgcagttgc cgctggtctg gatggcgtgc atctgcacgg
     1141 ccgccagatg ggccaggatg tcgggcacct cctccaggcg cggccagttg aactggatgt
     1201 tctggcgcgt gctgaagtgg ccgtagccgc gatcgtaagt gcgggcgatg aaggcgagct
     1261 tgcgcagctg cgccgtggac agcaggccgt aggggatcgc cacccgcagc atcggcgcgt
     1321 gcttctggat gtacagcccg ttgcgcaggc gcagcgggcg gaactcgtcc tccgacagcc
     1381 gccccgccag gaaacggcgc gtctggtcgc gatactgcgc gacgcgctcg tccaccaggc
     1441 gctggtcgat cggatcgtag aggtacatgg ggcgtcagtg cacgagttcg aaaccgggtt
     1501 gagacggaaa cgctggcgcg ggagtcgacc ggagggtcaa cagcccgacg cagcgcgcgc
     1561 ggcacacgcg caaccgcggc gggcggcgcg catgcgcatg gaagccggag agatttgcag
     1621 gtggtgcgag tgcggggtcc gagcgacgac tggccgcggg ccgcagcccg cctgcctgtc
     1681 cccgtcgtcg tgcgcccgta aggagctcat cccgctaccc gtgcagtagg tgagcggaat
     1741 gctagcaagc ccttatagct tgtgaaacga atatgaagtc atatcgttat gctcaagggc
     1801 tatatgctcg ccccctcacc cccgccctct cccgcagggg gagagggggc tatttgcgtg
     1861 gcggacgccg ggtcggcttc gtgccttccg cgccggccgc gctcgcctgg cgcgcgcggt
     1921 ccaccaccgc gcgcgtcaga tgcggcgcga aggcgtggat gaaggcgtag gtgtacccgc
     1981 gcaggtaggc ggtgcgcttg accgcgacgc gcgtggtgtt ggtcccgaac agatgcccgg
     2041 cgtcgatggc gctcaagccg ggatcgcgtt tgcgatcgac cgccatcgcg gcgacgatcc
     2101 ccacgccgag gccgagctcg gtataggtct tgatcacgtc cgaatccagc gccgtgagca
     2161 cgatgtcggg tttcaggccc ttggccgcga atgcttcgtc gatgtgcgtg cggcggctga
     2221 acgcggggct gtaggtgatc agcgggtact tggccagctc atcgagagtc aggcgcgtca
     2281 ccttgctcag ggcatgcccg cgcgggacga tggcgcagtg gtgccaggtg tagccgggca
     2341 gggcgagcag atcgggatag tggtccagcg cctcggtggc gatggcgatg tcggcgcggc
     2401 cttcgcagac catgtcggcg atctgggtcg gcgtgccctg cagcagcgac aggtgcacgt
     2461 tgggatactg gatgcggaaa cgctgcacca cgaccggcag ggaatagcgc gcctgcgtgc
     2521 tggtggtggc gatggtgagc gcgccgctgg cctggtcggc gaactccttg ccgacctgcc
     2581 gcagattgtc ggcttcgtcc agcaggcggt cgatcacccg caccacgagc tgccccggct
     2641 cggtcaacgc gaccaggcgc ttgccgcgac ggacgaagat ctcgatcccg agctcgtcct
     2701 ccagctcgcg gatctgcttg ctgatgccgg gctgcgaggt gtacagagcg ttggccgcct
     2761 cggtcaggtt cagcccctgg cgcaccgcct cgcgcacgta gcgcaactgc tggagattca
     2821 tgcgatctgc ggtatggcgg cgttatgctc cgccgcccgt gtggtttctc ctccttataa
     2881 gcttagctta tgacagcgcg gcttgtcccg tgcaggatcg acggctcgac cgggctgcgg
     2941 tccagaaaca cctgcatccg ccgcgggcgg atcagcagcg tttcgcccac cttgaggttc
     3001 aggccgcgcg cccggggggc ggggatctcg acctccagga tctcattgct gtcttcgcgc
     3061 tcgagctcca gtcgcgcggt gggaccgacc accagcatgc ggctcagttg caccggcagg
     3121 ccgtccagcc cgggcgagaa gcgctcgatc tcgatctcgt gcgggcgcgc gtaggcgacg
     3181 gccggcgcgt tgcgcgcgtc gcggtgctcg ggcgcttcca ccgacaccgt ccccagctcg
     3241 aagcggccgt tgctggcgcg gccgtggaac acgttcaccg tgcccaggaa gccgtacacg
     3301 aaaggcgagg ccgggcgttc gtacacctcg tcgggggtgc cgatctgctc gaccttaccc
     3361 ttgttcatca gcaccacctg atcggcgacc tcgagcgcct cctcctgatc gtgcgtgacg
     3421 aagatgctgg tgatgtgcag ctcgtcgtgc aggcgccgca gccagcggcg cagctccttg
     3481 cgcacctttg catccagggc gccgaacggc tcgtcgagca gcagcacctt cggctcgacc
     3541 gccagcgcgc gggccagtgc gatgcgctgg cgctggccgc ccgagagctg gtgcgggtag
     3601 cgatcggcga gccagtcgag ctgcaccagc ttcagcagat ccagcacctt gcggcggatc
     3661 tgctcgttgg agggacgctc ggcgcggcgt ttgacgcgca gcccgaacgc cacgttctcg
     3721 aacacgctca tgtggcggaa cagcgcgtag tgctggaaca cgaagccgac ctggcgctcg
     3781 cgcacgtcct gccaggaggc gtcctcgccg tggaacagca cggcgccgct gtccggcgtc
     3841 tccaggccgg cgatgacccg cagcagagtg gtcttgccgc agccggacgg accgagcagg
     3901 gccaccagct gaccggaggg gaacgacagg gtcacgtcgt ccagcgccgt gaatgccccg
     3961 aaccgcttga ccaggtttct gacttcgatg ctcatggcga cgtcctcgac aaagtccccg
     4021 cccggccgct catgcgggcc gctcgctgga cagctcggcc gccagcgcca gctcgcgccg
     4081 cgccctccac tcgaccaggc tcttcagcag caaggtgacc agcgcgagcg aggcgagcag
     4141 cgaagcgacg gcgaaggcgg ccgcgaagtt gtactcgttg tagagaatct cgacgtgcag
     4201 cggcgtggtg ttggtgaccc cgcgcacgtg accggacacc accgagacgg cgccgaactc
     4261 gcccatggcc cgggcgttgc acaggatcac gccgtacagc aggccccact tgatgttcgg
     4321 cagcgtcacg cgccagaaca tctgccagcc cgaggcgccg agcacgcgcg cggcctcctc
     4381 ctcttcggtg ccctgcgcct gcatcagcgg gatcagctcg cgcgccacga acgggaaggt
     4441 gacgaagatg gtcgccagca cgatgcccgg cacggcgaac acgatccgga tgtcgtgcgc
     4501 ctgcagccaa gggcccagcc agccctgcgc cccgaacagc agcacgtaga tcaggccggc
     4561 gatcaccggc gagacggcga acggcaggtc gatgaacgtg atcagcacgc tcttcccgcg
     4621 gaagtcgaac ttggcgatgg cccaggcggc ggccacgccg aacaccaggt tcgccgggac
     4681 ggcgatggcc gccaccagca gcgtgagacg gatcgcggcg atggcatcgg gttcggtgag
     4741 tgccgcgaag taggtctcga cgcctttgcg cgtggcttcc gtgaacactg ccgccagcgg
     4801 cacaagtagg aacaccccga ggaaggcgag cgcgagtccg atcagcaacc agcgcacgta
     4861 ggcgggctcg gccgtcgcgc gattgcgggc ggcggccggg gccgccaggg gcagcatcgc
     4921 ggccgtcatc ctcgggtctg tcccatgcgc cggccgctcc accactgcag cagattgatg
     4981 agcagcagca gcgcgaacga gatcaccagc atgagcacgg cgacggacgt cgcgccggcg
     5041 tagtcgtact gctccagctt gatgatgatc agcagaggcg tgatctcgga gaccatcggc
     5101 atgttgccgg cgatgaagat caccgaaccg tactcgccga ccgcgcgcgc gaacgcgagg
     5161 gtgaagccgg tgagcagcgc tggccagacc gcaggcagga tgatgcgctg gaaggtctgc
     5221 aggcgattgg cgcccagcgt cgccgccgct tcctccagct ccggctccag atcctccagc
     5281 accggttgca ccgtgcgcac gacgaacggc aggccgatga aggtcagcgc gacgatcacg
     5341 cccagagggg tgaacgcgac cttgatcccg agcggctcga gatactggcc gagccagccg
     5401 ttgccggcgt acaccgcggt cagggcgatg ccggccaccg ccgtgggcag ggcgaagggc
     5461 agatccacca gcgcatcgca cacgcggcgc ccgaagaacc ggtagcgcac cagcacccac
     5521 gcgaccagca gcccgaacac ggcgttgatc agcgcggcgg cgagcgacgc accgaacgac
     5581 aggcgcagcg aagcgagcac gcgcggcgcg gtgaccgtgg tccagaacgc gtcccagccc
     5641 aggccggccg acttgaagaa cgtcgccgaa agcgggatca gcacgatcag cgacaggtac
     5701 aggacggtgt atccgagcgc gagcccgaag ccgggcagca cattcgggcg cctggacaac
     5761 ccgcctggaa gagcggcgat cgacgtcgat gcggtcgtca tagcgcagca attcgctccc
     5821 cgcctctcac gag
//
LOCUS       DASPGE010000104         5327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_617370, whole genome shotgun sequence.
ACCESSION   DASPGE010000104 DASPGE010000000
VERSION     DASPGE010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617370"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1259)
                     /locus_tag="VD791_03325"
     CDS             complement(48..1259)
                     /locus_tag="VD791_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HXV07032.1"
                     /translation="MTSLDFLPSLPLVAGDLALIGAILAIGLVSGELFRRLLRLPRIS
                     GYLLVGIALGPGALDLLGRDLLVAARVVLDVALGLILYELGSRLDLGWVRRNRWLLVT
                     SVAESALSFALVYLALRWLAVLPLHAAVAAAIAMSTSPSVLLLVIHDERAEGALTEQA
                     LNLTALNNIFAVVTVTMLLAYMHLEYQAGVHIMLLHPVYLLLGSLAVGYAAAALTVLF
                     AGWLGKREDLQFVMLVSMVLLAVGSALALRVSVLLTLLAFGVLARNRDRRRVLLPVEF
                     GPAAQFFFVALFVLTGAYLEWKDLRLVGGAALVYVIARVAGKLLGILVFAPLTGLRVR
                     KAALLGLVLTPMAAVAVVLVQTTSEIYPAFGAELGAIVLSAVVVLAVAGPIAAQFAFR
                     LAGETHREAAR"
     gene            1408..2610
                     /locus_tag="VD791_03330"
     CDS             1408..2610
                     /locus_tag="VD791_03330"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXV07033.1"
                     /translation="MQLPPARWLTRLVVGATVISANSGAEADGPAREPLGALLLPLVS
                     TRFQPQLLLARRAATILYRVRLVAAAFALLTVAWIPVDALMLDPGTRTPVAIARLVAA
                     CAFLVLALAAWRANSLQAARLAVAILVLIPSAFFVFAATQWTQATLADESFGLRGVYA
                     AVPFVVASGLGLFPLTAIEAAAASLPIFLAEAGVAALDIQALTPMETLGSLWLLALVT
                     GVAGLAGMAQVGLMSQLLRQMARDPLTGAYRRATGEELLALEMGLARRSDIPLAVLFI
                     DLDRFKSINDQFGHEVGDQVLQHAARAILDQMREGDLLVRWGGEEFVVVLPNMRCADA
                     GRFLERVRATGLGARPDGKTVTASVGISERLADAAAEWWQLVDLADRRMYEAKRAGRD
                     RYVGCGGT"
     gene            complement(2693..3358)
                     /locus_tag="VD791_03335"
     CDS             complement(2693..3358)
                     /locus_tag="VD791_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HXV07034.1"
                     /translation="MASARDNILGRIRSALGRAQAPASGSDEAGAAGMRARLARHPRG
                     PLPSMDWEPVARFEERCLLLSSSVARVRNRSEVAPAVAQYLAQNDLPRSGVCWPELGG
                     LGWSATGLRIEARSAGGEDKVGITGCYCAIAETGTLMLLSGPDTPLTASLLPDTHIAL
                     VPAPRILRSMEDAWDLLRRESGALPRQVAFVSGPSRTADIEMTLVLGIHGPYRVHLIL
                     VDE"
     gene            complement(3368..4315)
                     /locus_tag="VD791_03340"
     CDS             complement(3368..4315)
                     /locus_tag="VD791_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HXV07035.1"
                     /translation="MHHIVFLDRAILPIALPQPGFAHSWVEYPTTAPGELDARLQGAT
                     IVISSKIPLRGETLARFPGIRFIAVAGTGVDVFDLDWCREHGVPVSNVRGYAEHTVPE
                     HAFMLILALARNLPGYRGDVEAGQWQRAEPFCLLTRPIFDLHGKTLGIVGEGAIGQGA
                     ARIARGFGMRVLFADHAPPKAPGIDYTPFDELLAGSDVVTLHCPLTPATRGLIGEHEL
                     RRMKRSALLINTARGGLVDERALVHALQEGWIAGAGVDVLSVEPPRDGNPLLELRLPN
                     LIVTPHVAWASREAMQAVAAQLIGNIEAFVAGRPRNLVV"
     gene            complement(4315..>5327)
                     /locus_tag="VD791_03345"
     CDS             complement(4315..>5327)
                     /locus_tag="VD791_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HXV07036.1"
                     /translation="DLGEYLIEQSGDVPSHIIAPAVHRTKDEVADLFAQRHNLPRKTD
                     IGELTREARMMLRPHFLTADMGITGANFLIAETGSTMIVTNEGNGRMTTTLPRVHVAI
                     AGIEKVLPTLEDVSFILRLLTRSATAQSISNYLTFTTGPKQATDADGPEEFHVVIVDS
                     GRTQVLGSELREALRCIRCGACMNHCPVYQNIGGHPYGWVYPGPIGSVLTPAYAGLEN
                     AVDLPNAATLCNQCGVVCPVKIPLPDLMRKLRERQVQRGLRPWPERLALHAWAWAAVR
                     PSLYALLARIGARLGRMAGGRDGLIHHLPGATAWTAQRDMPAPNGRTFRELYAIRRDR
                     KA"
BASE COUNT          845 a         1867 c         1862 g          753 t
ORIGIN      
        1 ttccacgccg acagtgaacg gctcggagcg gcggaaatcg agaatgctca tcgcgctgcc
       61 tcccgatgcg tctcgcccgc caggcggaac gcgaactggg ccgcgatcgg cccggccacg
      121 gccagcacca ccaccgcgga cagcacgatg gcgccgagct ccgcgccgaa cgccggatag
      181 atctcgctgg tggtctgcac cagcaccacg gcgaccgcgg ccatcggcgt gagcaccagg
      241 ccgagcagcg ccgccttgcg cacccgcagc cctgtgagcg gagcgaacac caggatgccg
      301 aggagcttgc cggcgacccg cgcgatcacg tagaccagcg ccgctccgcc caccagcctg
      361 aggtccttcc actccagata ggcgccggtc agcacgaaca gcgctacgaa gaagaactgc
      421 gcggccggcc cgaactcgac cggcagcaag acgcgcctgc ggtcgcggtt gcgcgccaac
      481 accccgaagg ccagcagcgt cagcagcacc gacacgcgca gcgccagcgc gctgcccacc
      541 gccagcaata ccatggacac cagcatcacg aactgcaggt cctcgcgctt gccgagccag
      601 ccggcgaaca gcacggtgag cgcggccgcc gcgtagccca cggcgagcga accgagcagc
      661 aggtacaccg ggtgcaatag catgatgtgg accccggcct gatactccaa gtgcatgtac
      721 gcgagcagca tggtgacggt caccacggcg aagatgttgt tgagagcggt gaggttcagc
      781 gcctgctcgg tgagcgctcc ctcggcgcgc tcatcgtgga tcaccagcag cagcaccgaa
      841 ggcgaggtgc tcatggcgat ggccgcggcc accgcagcgt gcagaggcaa gaccgccagc
      901 caacgcagcg caaggtacac cagcgcgaag gacagcgcgc tctcggccac gctggtcacg
      961 agcagccagc ggttgcgacg cacccagccc aggtccagcc gcgaacccag ctcgtagaga
     1021 atcagtccca gcgccacgtc gagaaccacg cgcgcggcga cgagcagatc gcggccgagc
     1081 agatccagag caccgggccc gagcgcgatc cccaccagca gatagccgct gatgcgcggc
     1141 agccgcagca ggcgccggaa caactccccg ctgaccaggc cgatcgccag gatggcgccg
     1201 atcagcgcca gatctcccgc caccagggga agggacggca ggaagtcgag cgaggtcatg
     1261 ccggaatcgg tatgcgcgcg agcatggacc gatcatcgcc caggcgcctg caaaccgcaa
     1321 ccgccgatgc cccgcaaccg tcgatgtcac ggcgcgggct tgtgtccggg ccgttacaat
     1381 ggtaggcaac gtgcgcgagg aagatgcatg cagctgcccc cggcccgctg gctcacgcgc
     1441 ctggtcgtcg gcgcgacagt gatctcggcg aattccggcg ccgaggcaga cggtcctgcg
     1501 cgcgagcctc tgggcgcgct gctgctgccg ctagtctcca cgcgctttca gccgcagctt
     1561 ctgctcgcgc gccgcgccgc aacgatcctt taccgggtgc gactggtcgc ggccgcgttc
     1621 gcgctgctga ccgtggcgtg gatcccggtg gatgcactga tgcttgaccc aggcacgcgc
     1681 actccggtgg cgatcgcccg gctggtggcg gcctgcgcgt tcctggtgct ggctctggcc
     1741 gcgtggcgcg cgaattccct gcaggccgcc cgcttggcgg tggcgattct ggtcctgatt
     1801 ccgtcggcct tcttcgtgtt cgccgccacg cagtggacgc aggccactct ggccgacgag
     1861 agcttcggcc tgcgcggcgt gtacgccgcg gtgccgttcg tggtcgcgag cggtctcggg
     1921 ctgtttccac tcaccgcgat cgaggcggct gccgcctcgc tgccgatctt cctggccgaa
     1981 gccggggtgg cggcgctcga catccaagcg ctcacgccga tggagacgct cggctcgctg
     2041 tggctgctgg cgctggtgac cggcgtggcc gggctcgccg gcatggcgca ggtgggcctg
     2101 atgtcgcagc tgctgcggca gatggcgcgc gacccgctca ccggcgccta ccggcgcgcg
     2161 accggcgagg agctgctggc gctggagatg ggcctcgcgc gccgcagcga catcccgctc
     2221 gccgtgttgt tcatcgacct ggaccgtttc aagtcgatca acgatcagtt cgggcacgaa
     2281 gtcggcgatc aggtgctgca acacgccgcc cgggccatcc tcgatcagat gcgcgagggc
     2341 gacctgctgg tgcgctgggg cggagaggag ttcgtcgtcg tgctgccgaa catgcgctgc
     2401 gcggatgccg gccgcttcct ggagagggtg cgcgcaaccg gactgggtgc acgtccggac
     2461 ggaaagacgg tgacggcgag cgtcggcatc agcgaacgcc tggccgatgc ggccgccgag
     2521 tggtggcagc tggtcgacct ggcggaccgc cgcatgtacg aggcgaaacg cgccggccgc
     2581 gatcgctacg tcggctgcgg cggaacgtag cccggatgga gccgcccaag catcttgttg
     2641 acagagcggg cggcggaatc cggggaggtg aagcgcattt cacgtattcc cctcactcgt
     2701 ccaccaggat caggtgcacc cggtacgggc cgtggatgcc gagcaccagc gtcatctcga
     2761 tgtcggcggt gcgcgagggg cccgagacga acgcgacctg gcgcggcaat gcgcccgact
     2821 cacggcgcag caggtcccag gcgtcttcca tgctgcgcag gatgcgcggg gccggcacca
     2881 gtgcgatgtg cgtgtccggc agcaggctgg cggtaagagg cgtatcgggc cccgacagca
     2941 gcatcagcgt tccggtctcg gcgatcgcgc agtagcagcc cgtgatgccg accttgtcct
     3001 cgccgccggc ggaccgcgcc tcgatccgca ggccggtggc gctccagccc agcccgccca
     3061 gctcgggcca gcacacgccg gaacgcggca gatcgttttg cgccagatac tgcgcgaccg
     3121 ccggcgcaac ctcagagcga ttgcgcaccc gcgcgacgct gctcgacagc aacaggcagc
     3181 gctcctcgaa gcgcgctacg ggctcccagt ccatcgacgg cagcgggccg cgcggatgcc
     3241 tggcgagccg cgcgcgcatg ccggcagcgc ccgcttcgtc actccccgaa gccggcgcct
     3301 gcgcacgccc cagcgcgctg cggatgcggc ccaggatgtt gtctctcgcg ctcgccatct
     3361 tcgcccgcta gacgaccaga ttccgcggcc ggccggcgac gaacgcctcg atgttgccga
     3421 tcagctgcgc ggcgaccgcc tgcatcgcct cgcggctggc ccatgccacg tgcggcgtga
     3481 cgatcagatt gggcaggcgc agctcgagca gcgggttgcc gtcgcgcggc ggctcgacgc
     3541 tcagcacgtc cacgccggcg cccgcgatcc agccttcctg caacgcgtgc accagggcgc
     3601 gctcgtccac cagtccgccg cgcgcagtgt tgatcagcag cgcgctgcgc ttcatgcgcc
     3661 gcagctcgtg ctcgccgatc aaaccgcggg tggctggagt gagcgggcaa tgcagcgtca
     3721 ccacgtcgct gccggcgagc agctcgtcga acggcgtgta gtcgatgccc ggcgccttgg
     3781 gcggcgcatg gtcggcgaac agcacgcgca tgccgaaccc gcgcgcgatg cgcgccgcgc
     3841 cctgtccgat ggcgccttcg ccgacgatgc cgagcgtctt cccgtgcaga tcgaagatcg
     3901 gccgcgtcag caggcagaag ggttcggccc gctgccactg tcccgcctcc acgtcgccgc
     3961 ggtagccggg cagattgcgc gccagcgcca ggatcagcat gaaggcgtgc tccggcacgg
     4021 tgtgctcggc atagccgcgc acgttggaca ccggcacgcc gtgttcccgg caccagtcga
     4081 ggtcgaagac gtccacgccg gtgcccgcca ccgcgatgaa gcggatgccg ggaaacctgg
     4141 ccagcgtctc gccgcgcagc gggatcttgc tggagatgac gatggtcgcg ccctgcagcc
     4201 gcgcatccag ctcgcccggc gcggtggtcg ggtactcgac ccaggagtgc gcgaagcccg
     4261 gctgcggcag agcgataggc aggatggcgc ggtcgaggaa gacgatgtgg tgcatcacgc
     4321 ttttcgatca cgccttatcg catacaactc ccgaaacgtc cgcccattgg gcgccggcat
     4381 gtcgcgctgc gcggtccagg cggtcgcacc gggcagatga tggatcagcc cgtcgcggcc
     4441 gccggccatg cgtcccagcc gcgcgccgat gcgcgcgagc agcgcgtaca gcgatggccg
     4501 cacggcggcc caggcccacg cgtgcagcgc caggcgctcc ggccaggggc gcagcccgcg
     4561 ctgcacttgc cgctcgcgca gtttacgcat gaggtcgggc agcgggatct tcaccgggca
     4621 caccacgccg cactgattgc acagcgtggc ggcgttgggc agatccacgg cattctccag
     4681 accggcgtaa gcgggcgtga gcaccgaccc gatcggcccg gggtagaccc agccgtaggg
     4741 atggccaccg atgttctggt acaccgggca gtggttcatg caagcaccgc agcggatgca
     4801 gcgcagcgcc tcgcgcagct ccgagccgag cacctgggtg cggccggaat cgacgatgac
     4861 cacatggaat tcctcgggcc cgtccgcgtc tgtcgcctgc ttcggcccgg tggtgaaggt
     4921 caggtagttg ctgatcgact gggcggtggc cgagcgcgtg agcagacgca ggatgaagga
     4981 tacgtcctcc agcgtaggca gcaccttctc gatgccggcg atcgccacgt gcacgcgcgg
     5041 cagcgtcgtg gtcatgcggc cgttgccctc gttggtcacg atcatggtgc tgccggtctc
     5101 ggcgatcagg aagttcgcgc cggtgatgcc catgtcggcg gtgaggaagt gcgggcgcag
     5161 catcatgcgt gcctcgcgcg tgagctcgcc gatgtcggtc ttgcgcggca gattgtgccg
     5221 ctgggcgaac aggtcggcga cttcgtcctt ggtgcggtgc acggccgggg cgatgatgtg
     5281 ggaaggcacg tcgcccgact gctcgatgag gtactcgccg agatcgg
//
LOCUS       DASPGE010000105         3342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_382764, whole genome shotgun sequence.
ACCESSION   DASPGE010000105 DASPGE010000000
VERSION     DASPGE010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382764"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..807
                     /locus_tag="VD791_03350"
     CDS             229..807
                     /locus_tag="VD791_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07037.1"
                     /translation="MTVKRFHSTGGGAPHHGVEVRGDWLARLHPLFLASACIRGALAL
                     LVVLVAIYVLRLLGTSDLVAWLVLGLTSAGLAVVLLDDAFDIEGKVAEQAARAAVFEP
                     DGFGVRAAAVREYADDFLRIKGRIPFGRARLRQPGDDRPFRVYFPTRRADCGRWCARA
                     LLASLYNALRFIAIGLAVMMSIQLYFLSAGWA"
     gene            995..3292
                     /gene="pbpC"
                     /locus_tag="VD791_03355"
     CDS             995..3292
                     /gene="pbpC"
                     /locus_tag="VD791_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HXV07038.1"
                     /translation="MLRFAAPILSPRGTGALLALALASAAPALALPTYDEVRKAHVST
                     EGVLLDRHGEVIHELRVDMRGRRLEWVTLDGVSPAFLRAVVRAEDKRFYEHAGVDWLA
                     LTDAALDSMFSSKPRGASTLSMQVAAMLEASLKPRKQRRTVGQKWDQIQAARDLEKKW
                     TKRQILEAYLNLSTFRGEVQGVGAAARALFGKDASGLDEQEALILAVLLRGPNAQPPV
                     VVKRACGLAQSMQPPLACDGLAELAELRLSDVPNLVPAAALAPHVARELVSAEARRVE
                     STLDAGLQAVALDAAGRQLAALGGQNVADAAVLAVDNRSGEVLAYVGNAEEISSARFV
                     DGVRAPRQAGSALKPFLYELALEQRLLTAASLLDDSPVAIVTPAGLYVPQNYDRDFKG
                     MVSLRTALSGSLNVPAVRTLALLGPDLFVERLRALGFDAVRDDGEHYGYSLALGSAEV
                     TLWQLANAYRTLANGGLASPMRLTPGERARGQRVLDPAAAHIVTDILADRLSRSITFG
                     LDSPLAPRFWAAVKTGTSKDMRDNWCVGFTARYTVAVWVGNFDGSAMWDVSGVSGAAP
                     LWLEMINALHQNVPSHAPPDPPGVERVPITFEAALEPDREELFLPGTQMRRVVHKGQG
                     GGHPAIVYPASGQIIAVDPDIPPDTQRVRFRAVGVNGDARWRLNGEPLADASSWHPAP
                     GRWRLVLEDAAGVALDEVEFEVRGAPADVQGAADARNALGGGNGARVDPAGTAPASPA
                     NPSQPNRSSPDRSAPNPSAGGATRP"
BASE COUNT          451 a         1151 c         1218 g          522 t
ORIGIN      
        1 cgccggcgca cccgggtgac gggcaacgct ctcgccacaa tcgtttgacg cagccgcggc
       61 ggtcggtggc actgctccgg ccgcgcttgt cgcgatcgag cgaccgctgg cgggatcgat
      121 tgtcaagatt cggtggcgga aatccacgcg ccactatgcc gtcgcggttt ccggccgata
      181 ccggtacagc acacccgttt acgccgttcc agcaaacgaa ggcgcgagat gacggtcaag
      241 cggtttcatt cgactggcgg cggcgcaccg caccacggcg tggaggtgcg gggcgattgg
      301 ctggcccggc tgcatccgct gttcctggcg agcgcatgca ttcgcggcgc gctcgcgctc
      361 ctggtggtgc tggtcgcgat ctatgtcctg agactgctcg gcacgtccga tctggtggcg
      421 tggctcgtcc tcgggctcac cagcgccggg ctggcggtgg tcctgctgga cgacgcgttc
      481 gacatcgaag gcaaggtcgc cgagcaggcg gcgcgcgcgg cggtgttcga gccggacgga
      541 ttcggcgtgc gcgcggcggc ggtgcgcgag tacgccgacg attttctgcg catcaaggga
      601 cgcatcccgt tcggccgcgc gcggctgcgg cagcccggcg acgaccgacc gttccgggtg
      661 tacttcccga cgcgccgggc ggactgcggg cgttggtgtg cgcgggcgct gctggcctcg
      721 ctgtacaatg ccttgcgctt catcgccatc ggcctcgcgg tgatgatgtc gatccagcta
      781 tacttcctgt ccgcgggctg ggcctagcct tgacggaacc tggggccttg acgggacctg
      841 gggccttgac ctcaggggac aggctgccgt ccatccaccg agcgcccggc cgagcgagaa
      901 ctgcctggag ctcatcatct ccttcgggaa caccggatcc ggcgcgcgcg ggcccgcggc
      961 tcgggcgagg ccgacgagcg gcgccgatcg cgcgatgctt cgcttcgcgg cacccatcct
     1021 gagcccgcgc ggcaccggcg cgcttctcgc gctcgccctg gcgagcgcag cgcccgctct
     1081 ggcgctgccc acctacgacg aggtgcgcaa ggcgcacgtg agcaccgaag gcgtgctcct
     1141 ggaccggcat ggcgaagtga tccacgagtt gcgcgtcgac atgcgcggcc ggcggctgga
     1201 atgggtgacc ctggacggcg tgtcgccggc ctttttgcga gcggtggtgc gggcggagga
     1261 caagcgcttc tacgagcacg ccggtgtgga ctggctggcg ctgaccgacg cggcgctgga
     1321 cagcatgttc tcctccaagc cgcgcggggc gagcacgctg tcgatgcagg tggcggccat
     1381 gctcgaggcg agcctcaaac cgcgcaagca acgccgcacc gtggggcaga agtgggatca
     1441 gatccaggcc gcgcgcgatc tggagaagaa atggaccaag cgccagatcc tggaggcgta
     1501 tctcaatctg tccaccttcc gcggtgaggt gcagggcgtg ggcgcggcgg cgcgcgcgct
     1561 gttcggcaag gacgccagcg gcctggacga gcaggaggcg ctgatcctgg ccgttctgct
     1621 gcgcggtccc aatgcgcagc cgccggtggt ggtgaagcgc gcctgcgggc tggcgcagtc
     1681 gatgcagccg cctctggcgt gtgacggtct ggccgagctg gccgagctgc ggctttccga
     1741 cgtgccgaac ctggtcccgg ctgctgcgct cgcgccgcac gtcgcgcgcg agctggtgag
     1801 cgccgaggcg cgccgcgtcg agtccacgct cgacgcggga ctgcaagctg tggcgctcga
     1861 cgcggcgggg cgccagctcg ctgcgctagg cgggcagaat gtcgccgacg ccgccgtgct
     1921 cgcggtcgac aaccgcagcg gagaggtgct ggcctacgtg ggcaacgccg aggagatctc
     1981 cagcgcgcgc ttcgtggacg gagtgcgcgc gccgcgtcag gccggttccg ccttgaaacc
     2041 gttcctctac gagctggcgc tcgagcagcg gctgctcacc gcggcgtctc tgctggacga
     2101 ttcgccggtc gctatcgtca cgccggcggg cctctacgtg ccgcagaact acgatcgcga
     2161 cttcaagggg atggtgagcc tgcgcacggc gctgtcgggc tcgctcaacg tgccggccgt
     2221 gcgcacgctc gcgctgctgg gcccggatct gttcgtggag cgtttgcgcg cgctcggctt
     2281 cgacgcggtg cgcgatgacg gcgagcacta cggttactcg ctggccctgg gttcggccga
     2341 ggtcacgctg tggcagctgg ccaacgccta tcgcacgctg gccaacggcg gcctggcctc
     2401 gccgatgcgg ctcacgcctg gagagcgcgc acgcggtcaa cgcgtgctgg atccggccgc
     2461 ggctcacatc gtgaccgata tcctcgccga ccggctgtcg cgcagcatca ccttcggcct
     2521 ggacagcccg ctcgcgccgc gcttctgggc cgcggtgaag accggcacca gcaaggacat
     2581 gcgcgacaac tggtgcgttg ggttcacggc gcgctacacc gtcgcggtgt gggtcggcaa
     2641 tttcgacggc agcgccatgt gggacgtgtc cggcgtgagc ggtgcggcgc cgctgtggct
     2701 ggagatgatc aacgccttgc atcagaacgt ccccagccac gcgccgcccg atccgcccgg
     2761 tgtggaacgc gtcccgatca ccttcgaggc ggcgctcgag ccggaccgcg aggagctgtt
     2821 tctgcccggc acgcagatgc ggcgcgtggt gcacaagggg cagggcggcg gccaccctgc
     2881 catcgtctat cccgccagcg gtcagatcat cgcagtggac cccgatatcc cgccggatac
     2941 ccagcgcgtg cgattccggg ccgtcggggt gaacggcgac gcgcgctggc gcctgaacgg
     3001 cgaaccgctc gccgacgcgt cctcctggca ccctgcaccg gggcgctggc ggctggtcct
     3061 ggaggacgct gctggcgtcg ccctggacga agtcgagttc gaagtgcgcg gcgcaccggc
     3121 cgacgtccag ggtgcggccg acgcacgcaa cgcgctcggc ggcggcaacg gtgctcgcgt
     3181 cgaccctgcc ggaaccgccc cggcttcgcc ggcgaatccc tcgcagccga atcgctcgtc
     3241 accggatcgc tcggcaccaa atccctcggc agggggcgcg acccgcccgt gaaacccgtg
     3301 acgatcctgc tggcgctggc gtgcgccttg acgctcgcgc cc
//
LOCUS       DASPGE010000106        10551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_149106, whole genome shotgun sequence.
ACCESSION   DASPGE010000106 DASPGE010000000
VERSION     DASPGE010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10551
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149106"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="VD791_03360"
     CDS             <1..1042
                     /locus_tag="VD791_03360"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HXV07039.1"
                     /translation="PWRGLSVTLEGINVENLDGNDFTVTPSPTSPYPVIADPDDSDIN
                     QLYLDYTGVPKTLLRGGRQSIKIDNVRFIGNVEFRQNMQVFNAVGVENTSLRNIRLYA
                     GYLFQQKTILAAEREIDAPIFNARYTWRAGNNLIGYAYLQDQAVTGQNLNTGPVTGFT
                     DNSNKVLGVRADGTYPIGERWKLLYTAEYADQSDYSGGDARIDASYWHVGAGPQFGDW
                     YLRLDFEQLGSNDGLYGFQTPLGTNHLFQGWADQFLTTPRQGIEDLYLVAGGKILELG
                     LYAEVHRFESDFGGIDFGDEFDIGLTWPLLKGLTGKVEYADYRAGDAVPGDALANKVD
                     VRKFWLTLIYQF"
     gene            1225..1713
                     /locus_tag="VD791_03365"
     CDS             1225..1713
                     /locus_tag="VD791_03365"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HXV07040.1"
                     /translation="MDFVPALARWLHLLAGVMWIGLLYYFNFVQVAALKAAQADNTAA
                     GIGKHVAPRALLFFRWAAVVTWLAGAALLGGNFVAAFTFQGAYAAIGMGAWLGTIMLF
                     NVWVLIWPNQKKILGIVQASDEQKAKARRVAFLASRANTMLSIPMLFFMGAGGHSAIY
                     FH"
     gene            complement(1788..2222)
                     /locus_tag="VD791_03370"
     CDS             complement(1788..2222)
                     /locus_tag="VD791_03370"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HXV07041.1"
                     /translation="MLKPWTAGLTLAVAAGALLADPLLTYPLKIKSHTLRVEVARSDE
                     EKTLGLMFRKQLGADSGMLFVYETEGRWAMWMKNTYVPLSVAFIDRDGRILNIEDMEP
                     LTEDSHQAAGAAKYAIEVNRGWFRKRGIKPGDRVEGIGKIPG"
     gene            2446..4092
                     /locus_tag="VD791_03375"
     CDS             2446..4092
                     /locus_tag="VD791_03375"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXV07042.1"
                     /translation="MTASLLVVDDEALYREIIAAYLEPEGYALTMAENGEQALALLVN
                     GHAFDAVLLDRMMPGIDGIEVLKRVKRDPRFNALPVIMQTAADSHEQVAEGLRLGAHY
                     YLTKPYHRDALVAVVRSALSAVAQRQRLAEQIEEYRGVMALIDESVFRYRSIDEARAL
                     AAALASMCRDPAAAGFGLLELLINAVEHGNLGIRLEDKCELVATGRWEQEVTARFAKP
                     ENAAKSVEVHCRREGERLLIRIRDQGAGFDWRSFLAMDEASVFRPNGRGIALAREVAF
                     ETMEYSSPGNQVEVTVAARSSTLHGAGEAPRNDAESELELGAALMSSILQRHEGLRDP
                     ALAWAVLPSHRFSGDAVAAARTPSGRLLAMLADATGHGLQAAISVLPLLQVFYAMARR
                     DLSVAELTAEMNRRLRTFAPAGVFLAAVVVACDPAAGRVELWNGGMPGGLWLHADGEA
                     QPAALASRHLPLGILDDARFDGACATLEARRGRLAFFSDGLIEAGAPDGEPFGAARLR
                     RHLLDATPDPGFQRLLDAVRAHLRGAPAHDDISLMTIALD"
     gene            4324..6069
                     /locus_tag="VD791_03380"
     CDS             4324..6069
                     /locus_tag="VD791_03380"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HXV07043.1"
                     /translation="MVKQLSLNKAALAVAAAAALGASAQVSAQGVDTLESKLYPNYVT
                     DDMLLNADKDHSNWLHYGKDYQSTRYSALKQVNRDNVKRLRPVWNLSFGVLEGQDSQA
                     NVVNGTVYVTTSFNKVIAADGTTGKILWKYERELPGDVFPKLCCDVVNRGVALYKNKV
                     YLATLDAHIVALDNQTGKVVWDKKMGDYTYAETFTIMPMALQNKIIFGTAGAEYGVRG
                     WIKAINADTGELVWQTYTVPGPGEPGNDTWAGESWKYGGGSAWITGSYDAATNRLFWP
                     IGNPGPDFDRHVRLGDNLFTNSNVVMNPDTGKILSYYNYTPNDPYDYDGVNEMILVDV
                     GGKKLWLHGSRNGHIYGIDRDATVTGKAGKEHKCAWVTPLQRVNWTKPLHTGNNCKPV
                     YNWPEKDVVYDKVTKDIAPSLDGGKEWHPMAYSHRTKMVYVPVYDFAMDLQAKKMEWK
                     RGEWYLGAKVITFNAGAGHIKAFDAATGKLVWAKAQSYPATSGILTTGGGLMFYGDPE
                     GYFSALDDETGEHLWSFQVGSGVHGNPTTYTANGKQYVAIVYGAGGGGIWPLYYADWL
                     KKNTKGGGLMVFALD"
     gene            6265..7149
                     /locus_tag="VD791_03385"
     CDS             6265..7149
                     /locus_tag="VD791_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXV07044.1"
                     /translation="MNRTQHLFVAILALLALVAVRPAAAQQQAALEQAGPDLSDPNQI
                     KDALERVKARGKLIACADPYDWPYSSNAEDPPGFDIDIIRAVAKRSGLKLEMYWADTG
                     TRGGIMRAFRNSILAKRCDVFLGLSDNGEDDLLPGALTFTDAYISLGYVLVVQGKAEG
                     MKSLEDFKKAGMKVGVTMSTPIDDYLFMNQVPRELYLNNRRLMEGMQKGDVDAGMVWA
                     TAVAVAKQEFPKAKFHMVPDYVPLDAHRFNSKFAVRKEDKSLLQFINEGIKDLLVNGR
                     MKQIVESYGVPYYPPIAS"
     gene            7171..7626
                     /locus_tag="VD791_03390"
     CDS             7171..7626
                     /locus_tag="VD791_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV07045.1"
                     /translation="MFRVSSATLGRLATPALGVVALAGGLLLAQPAAAQHKMNENDME
                     LPVSDPKVVELGREKYAQRCSFCHGGAGKGGKGPCLTCGRFPYSGNTNTNIYTTIAVG
                     VTNRSQGGTMGAFGTTMSAEEILAVLTFMRAEEKRRIDEGEIPDPYKQE"
     gene            complement(7636..8082)
                     /gene="dut"
                     /locus_tag="VD791_03395"
     CDS             complement(7636..8082)
                     /gene="dut"
                     /locus_tag="VD791_03395"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HXV07046.1"
                     /translation="MRTIDIKILDARLNEFPPAYATAGAAGLDLRACIDEAVEIRPGQ
                     SELIASGIAIHLADPSLAAMVLPRSGLGHKHGIVLGNLVGLIDSDYQGQIFVSVWNRG
                     SAAFSLKPMERIAQLVIVPVLQVRFNRVQDFAQSARGIGGFGSTGR"
     gene            complement(8103..9299)
                     /gene="coaBC"
                     /locus_tag="VD791_03400"
     CDS             complement(8103..9299)
                     /gene="coaBC"
                     /locus_tag="VD791_03400"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HXV07047.1"
                     /translation="MTALPQHRILLGITGGVAAYKAAELCRLLVRDGMDVRVVMTDAA
                     CRFVAPVTMQALSGQPVLTDLWDASIPNAMGHIELSRDRDLIMVAPASADFLGKLAGG
                     LADDLLSTLCLARECPLLVAPAMNRQMWENAATQRNVERLRADGVVILGPGSGDQACG
                     EVGMGRMLEAADLHAAIVAALQPKLLAGVRVLVTAGPTFEAIDPVRGLTNRSSGRMGY
                     AVAQAALEAGAQVTLVSGPTALTAPAGARVVPVASAQDMLQAVSAEIAATDVFVAVAA
                     VADYRVKQASVQKIKKNDRPLTLELEPTVDILASVSTRPDPPFCVGFAAESHDLDRFA
                     QDKRKKKKLPLLVANLAQEAIGAQEVELILFDDAGRHPLPRAPKLEQARALVKHIAVL
                     YRARKA"
     gene            9399..10073
                     /gene="radC"
                     /locus_tag="VD791_03405"
     CDS             9399..10073
                     /gene="radC"
                     /locus_tag="VD791_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HXV07048.1"
                     /translation="MGIADWPQDRQPREKLLARGEAALSEPELLAIVLRTGVRGKSAV
                     DLARELLERFGTLHGLLEAEPEAIQRSSGLGPAKSAQLRAVLALVKRALGQEVQRRDN
                     LSSPQAVRDYLRLNLAQRDREVFVAVFLDAQNRVLKIQELFAGTLTQTSVFPRDVVRY
                     ALLANAAAVIFAHNHPSGLAEPSRADETLTQALKQALALVDVKVLDHFVVGAGGAMSF
                     AERGLL"
     gene            complement(10089..>10551)
                     /locus_tag="VD791_03410"
     CDS             complement(10089..>10551)
                     /locus_tag="VD791_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07049.1"
                     /translation="QLGAIELAHTGTLHRFERDAAGNWFYHGVHTASEAAHEHQTDPA
                     AATRIGQAFAALGRARIERELQAGARVNDYGVASPELLILAYRPKEPLPLVQYAVGDL
                     APDQFSRYVQAVGSSSVITIANYQIDNLLSLIQAVAGPADQTAAQDERGPR"
BASE COUNT         1786 a         3548 c         3570 g         1647 t
ORIGIN      
        1 gccctggagg ggcttgagcg tgacgctgga agggatcaac gtcgagaacc tggacggcaa
       61 cgacttcacc gtcacgccgt cgccgacctc accctacccg gtgatcgccg accccgatga
      121 cagcgacatc aatcagcttt acctcgacta caccggtgtg ccgaagacgc tgctgcgcgg
      181 cgggcgccag tcgatcaaga tcgacaacgt gcgtttcatc ggcaacgtcg aattccgcca
      241 gaacatgcag gtgttcaatg cggtgggcgt ggagaacacg agcctgcgca acatacggct
      301 gtacgcaggc tacctgttcc agcagaagac catcctcgcc gccgagcgcg agatcgacgc
      361 gccgatcttc aacgcgcgct atacctggcg cgcgggcaac aacctgatcg gctacgccta
      421 cctgcaggac caggcggtca ccggccagaa cctgaacacc gggccggtga ccggcttcac
      481 cgacaactcg aacaaggtcc tcggcgtgcg cgcggacggc acctacccga tcggcgagcg
      541 gtggaagctg ctgtacaccg ccgagtacgc cgatcagagc gattactccg gcggcgacgc
      601 gcgcatcgac gcgagctact ggcacgtggg ggccggccct cagttcggcg actggtacct
      661 gcgcctggac ttcgagcagc tcggcagcaa cgacgggctg tacggattcc agacgccgct
      721 cggcaccaat cacctgtttc agggctgggc cgatcagttc ctgaccaccc cccggcaagg
      781 catcgaggac ctgtacctgg tggccggcgg caagatcctc gagctcggcc tctacgccga
      841 ggtgcaccgc ttcgagtccg acttcggcgg catcgacttc ggcgacgagt tcgacatcgg
      901 gctcacctgg cccctgctaa aggggctgac cggcaaggtc gagtacgcgg attaccgcgc
      961 cggcgacgcg gtgccggggg acgcgctggc gaacaaggtg gacgtgcgca agttctggct
     1021 gaccctcatc tatcagttct gagcgggctg ccttcgacgc tctccgggcc tgctagactc
     1081 acgcctgcgg cattccaaat aaccacgccg aaaaggaggg catccatgaa tccgttggac
     1141 tcgctgtggg gaacgatcat cgcgggcttg gtcctgaccg ggattctgta cttcatcgtg
     1201 cgcgcgctgg cgggaggctg agccatggac ttcgtccctg ctctcgcacg ctggttgcac
     1261 ctgctggccg gggtgatgtg gatcggcctg ctgtactact tcaacttcgt gcaggtcgcg
     1321 gcgctcaagg cggcgcaggc ggacaacacc gccgcgggca tcggcaagca cgtcgcgccg
     1381 cgcgccctgc tgttcttccg ctgggcggcc gtggtcacct ggctggccgg tgcggcgctg
     1441 ctgggcggta acttcgtcgc ggcgttcacc ttccaggggg cctatgcggc catcggcatg
     1501 ggcgcatggc tcggcaccat catgctgttc aacgtctggg tgctgatctg gccgaaccag
     1561 aagaagatcc tgggcatcgt gcaggcgagc gacgagcaaa aggcgaaagc gcgccgcgtc
     1621 gcgttcctgg cctcacgcgc gaacaccatg ctgtcgatcc cgatgctgtt cttcatgggc
     1681 gccggcgggc actcggcgat ctatttccac tgagtcgcgc acgcggccgg tcggttcaag
     1741 ggcgcgcccg gtggcgcgcc ctttcgttta ctgcggcgct gcgcggttca cccagggatc
     1801 ttgccgatcc cctcgacccg gtccccgggc ttgatgccgc gcttgcgaaa ccagccgcgg
     1861 ttgacttcga tcgcgtactt ggctgcgccg gcggcctggt gcgagtcctc ggtcagcggc
     1921 tccatgtcct cgatgttgag gatgcgcccg tcgcggtcga tgaacgccac cgacagcggc
     1981 acgtaggtgt tcttcatcca catcgcccag cgcccttccg tttcgtacac gaacagcatt
     2041 ccgctgtccg cgccgagctg cttgcggaac atgagcccga gggtcttttc ctcgtcgctg
     2101 cgggcgacct ccacgcgcag ggtgtggctt ttgatcttga gcgggtaggt gagcagcggg
     2161 tccgcgagaa gcgcgcccgc ggccacggcc aacgtcaggc cggccgtcca gggtttcagc
     2221 aagttctcct ccgtcaaggg gttgcgccgg tgcagcgtta aagtatcaca gcgctcggcc
     2281 gataaatcca gcgcgggcaa tccgcgccgc ggcctcctcc tctcgccagc cccatgctca
     2341 atttccagcg cgcctgtgat cccaccatgc gcagtcgggc ccggggcgca gccgggcccg
     2401 gggcgcagcc gccgtctcgc cggtgaccga ttcgccaagc gcaatatgac ggccagcctg
     2461 ctggtggtgg acgacgaggc gctgtaccgc gagatcatcg cggcctacct cgagcccgag
     2521 ggctacgcgc tcaccatggc cgagaacggc gagcaggccc tggccctgct cgtcaacggc
     2581 cacgccttcg acgccgtgct gctcgaccgc atgatgccgg gcatcgacgg catcgaggtt
     2641 ctcaagcggg tgaagcgcga cccgcggttc aacgcgctgc cggtgatcat gcagaccgcg
     2701 gccgacagcc acgagcaggt cgccgaaggg ctgcgcctgg gcgcgcacta ttacctcacc
     2761 aaaccgtatc accgcgatgc gctggtggcc gtggtgcgct cggcgctgtc ggcggtggcg
     2821 cagcgccagc ggctcgcgga gcagatcgag gagtaccgcg gcgtgatggc gctgatcgac
     2881 gagagcgtgt tccgctaccg ctcgatcgac gaggcgcgcg cgctcgccgc ggcgctggcg
     2941 agcatgtgcc gcgacccggc ggcggccggc ttcgggctgc tcgagttgct gatcaacgcg
     3001 gtggagcacg gcaacctcgg gatccggctg gaggacaagt gcgagctggt cgccaccggg
     3061 cgctgggagc aggaggtcac agctcggttc gcgaagccgg agaacgccgc caagtccgtc
     3121 gaagtgcact gccggcgcga gggcgagcgc ctgttgattc gtatccgcga ccagggggcc
     3181 gggttcgact ggcgcagctt cctggctatg gatgaagcaa gcgtcttccg tcccaacggg
     3241 cgtggcatcg cactggcgcg cgaggtcgcg ttcgagacca tggaatacag cagcccgggc
     3301 aatcaggtgg aagtcaccgt ggccgcgcgc agttccacgc tgcatggcgc tggggaggcg
     3361 ccccgcaacg acgcggaatc cgagctggag ctgggcgcgg cgttgatgtc ctcgatcctg
     3421 cagcggcacg aggggctgcg cgacccggcg ctcgcctggg cggtgctgcc ctcgcaccgc
     3481 ttcagcggcg atgccgtggc ggcggcgcgc acgcccagcg gaaggctgct cgccatgctc
     3541 gccgacgcca ccggtcacgg actgcaggcg gcgatcagcg tgctgccgct gctgcaggtg
     3601 ttctacgcga tggcccgcag agacctttcc gtggccgagc tgaccgccga gatgaaccgg
     3661 cgcctgcgca cgttcgcgcc ggccggggtg ttcctggcgg cggtggtggt ggcctgcgac
     3721 ccggctgcgg ggcgggtcga gctgtggaac gggggcatgc ccggtggact gtggctgcac
     3781 gcggacgggg aggcgcagcc ggccgcgctc gcctcgcgac acctgccgct gggtatcctg
     3841 gacgacgcac gcttcgacgg cgcctgcgcc acgctcgagg cgcgccgcgg gcgcctggcg
     3901 ttcttctcgg acgggctgat cgaggcgggg gcgccggacg gcgagccctt cggcgcggcc
     3961 cgcctgcgcc ggcatctgct tgatgccacg ccggatcctg gcttccagcg cctgctcgat
     4021 gcggtgcgcg cgcacctgcg cggtgcgccg gcgcacgacg acatctcgct catgacgatc
     4081 gcgctcgact gagcgtgcat gctgcgtcgc aatagacgct cggctccccg gccgaagccg
     4141 cgtggaaact tgttgacaac gatttcccgc ttctttatcg ttggtcacgc tgtgcatagt
     4201 gcagcacgtt cttcttgaag acgtcagacg ttctctaaga agcaacaaat caataaccgg
     4261 cgcaagcgct cgcactgaag ttccgccgag cgcgacgcgc cactttcggg aggtgtaccc
     4321 caaatggtga agcaactgtc gctcaacaag gcggcgttgg ccgtcgccgc tgcagcggcg
     4381 ctcggcgctt cagcacaggt ctccgctcag ggtgtggata cgctggaatc gaagctgtat
     4441 ccgaactacg tgacggatga catgctgctc aacgccgaca aggaccacag caattggctg
     4501 cattacggaa aggactacca gtccacccgt tacagcgcgc tgaagcaggt gaacagggac
     4561 aacgtcaagc gcctgcgtcc ggtgtggaac ctctccttcg gcgtgctcga agggcaggac
     4621 agccaggcca acgtggtcaa cggcaccgtc tatgtaacca catccttcaa caaagtcatc
     4681 gccgccgacg gcaccaccgg caagatcctg tggaagtacg agcgcgagct ccccggcgac
     4741 gtgttcccga agctgtgctg cgacgtggtg aaccgcggcg tcgccctgta caagaacaag
     4801 gtctacctgg ccacgctcga cgcgcacatc gtcgcgctcg acaaccagac cggcaaggtg
     4861 gtctgggaca agaagatggg cgattacacc tacgccgaga ccttcaccat catgccgatg
     4921 gccctgcaga acaagatcat cttcggcacg gcaggcgccg agtacggcgt gcgcggctgg
     4981 atcaaggcga tcaacgccga caccggcgag ctggtgtggc agacctacac cgtccccggc
     5041 ccgggcgagc ctggcaacga cacctgggcg ggcgaatcgt ggaagtacgg cggcggctcg
     5101 gcctggatca ccggctccta cgacgcggcg accaaccgcc tgttctggcc catcggcaac
     5161 cccggcccgg acttcgaccg tcacgtgcgc ctcggtgaca acctgttcac caacagcaac
     5221 gtggtgatga atccggacac cggcaagatc ctctcgtact acaactacac gcccaacgat
     5281 ccgtacgact acgacggcgt gaacgagatg atcctggtgg acgtcggcgg caagaagctc
     5341 tggctgcacg gctcgcgcaa cggccacatc tacggcatcg accgcgacgc cacggtgacg
     5401 ggcaaagccg gcaaggagca caagtgcgcc tgggtcaccc cgctgcaacg cgtgaactgg
     5461 accaagccgc tgcacaccgg caacaactgc aagccggtgt acaactggcc cgagaaggac
     5521 gtggtgtacg acaaggtgac caaggacatc gccccctcgc tggacggcgg caaagagtgg
     5581 cacccgatgg cctacagcca ccgcaccaag atggtctatg tgccggtgta cgacttcgcc
     5641 atggacctgc aggccaagaa gatggaatgg aagcgcggtg agtggtatct gggcgcgaag
     5701 gtcatcacct tcaacgccgg cgcgggccac atcaaggcct tcgacgcggc caccggcaag
     5761 ctggtgtggg ccaaggcgca aagctacccc gccaccagcg gcatcctgac caccggcggc
     5821 ggcctgatgt tctacggcga tccggaaggc tacttcagcg cgcttgacga cgagaccggc
     5881 gagcacctgt ggagcttcca ggtcggctcg ggcgtccacg gcaaccccac cacctacacg
     5941 gccaatggca agcagtacgt cgccatcgtg tacggggcgg gcggcggcgg catctggccc
     6001 ctgtactacg ccgactggct gaagaagaac accaagggcg gcggcctgat ggtgttcgcg
     6061 ctcgactgac gcaggcggcc gcgggccgct gaccgtctcg caagcggtaa agtagaatac
     6121 ggggagggcg caagccctcc cttttttgtg gaagcccaca atcagaagcc caggcggcag
     6181 aagccccgac gggccgttgc gggcgccgac cgcgaggccg gcgcatcaga gaaaagcgaa
     6241 gtgccgaaca tgaagagacc agtcatgaac cgaactcagc acctattcgt tgcaatcctg
     6301 gccctgctgg cgcttgttgc cgtgcggccc gccgccgccc agcagcaggc ggcgctggag
     6361 caggcggggc ccgatctgtc cgatcccaac cagatcaagg acgccctgga acgggtcaag
     6421 gcccggggca agctgatcgc ctgcgccgat ccctacgact ggccctattc gtccaacgcc
     6481 gaggatccgc ccggcttcga catcgacatc atccgcgcgg tggccaagcg cagcggtctg
     6541 aagctggaga tgtactgggc cgacaccggc acgcgcgggg gcatcatgcg cgccttccgc
     6601 aactcgatcc tggccaagcg ttgcgacgtc ttcctcggat tgtcggacaa cggcgaggat
     6661 gacctgctgc ccggcgcgct caccttcacc gacgcctaca tcagcctggg ctacgtgctg
     6721 gtggtgcagg gcaaggccga gggcatgaaa tcgctcgagg acttcaagaa ggccgggatg
     6781 aaggtcggcg tgacgatgtc cacgccgatc gacgactacc tgttcatgaa ccaggtgccg
     6841 cgcgagctgt atctgaacaa ccgccgcctc atggagggca tgcagaaggg cgacgtggac
     6901 gccggcatgg tgtgggcgac cgcggtggcg gtggccaagc aggagttccc gaaggccaag
     6961 ttccacatgg tgcccgacta tgtcccgctc gacgcgcacc gcttcaacag caagttcgcg
     7021 gtgcgcaagg aggacaagtc gctgctgcag ttcatcaacg agggcatcaa ggacctgctg
     7081 gtgaacggtc gcatgaagca gatcgtcgaa agctacggcg tgccctacta tccaccgatc
     7141 gcgtcgtgac gtcttcaggg aggagctcag atgttccgcg tatcgagcgc aaccctcggc
     7201 aggctagcga cgccggcgct gggcgtcgtc gcgctcgccg gcggcctgct gctggcgcag
     7261 cccgcggccg cgcagcacaa gatgaacgag aacgacatgg aattgccggt gagcgacccg
     7321 aaggtggtcg agttgggccg cgagaagtac gcccagcgct gctccttctg tcacggcggc
     7381 gccggcaagg gcggcaaggg tccgtgcctg acgtgcggca ggttccccta ctcgggcaac
     7441 accaacacca acatctacac caccatcgcg gtgggggtca ccaaccgctc ccagggcggc
     7501 accatgggcg ccttcggcac caccatgagt gccgaggaga ttctcgccgt gctcaccttc
     7561 atgcgcgccg aggaaaagcg ccgcatcgac gagggcgaga tccccgatcc gtacaagcag
     7621 gagtgaagcg gcgcttcacc gccccgtact gccgaagccc ccgatgccgc gcgcgctctg
     7681 cgcgaagtcc tgcacgcggt tgaagcgcac ctgcaggacc ggcacgatca ccagttgcgc
     7741 gatgcgctcc atcggcttca ggctgaaggc ggcgctgccg cggttccaca ccgacacgaa
     7801 gatctgcccc tggtagtcgg agtcgatcaa cccgaccagg ttgcccagca cgatgccgtg
     7861 cttgtggccg agcccggagc gcggcagcac catggccgcc agactcggat cggcgaggtg
     7921 gatcgcgatg ccgctcgcga tcagctcgct ctgccccggt cggatctcga cggcctcgtc
     7981 gatgcacgcg cgcaggtcca ggccggcggc gccggcggtg gcgtaggccg gtgggaactc
     8041 gttcagcctg gcgtcgagga tcttgatgtc gatggtgcgc atgctcggca acggcgcacc
     8101 gatcacgcct ttcgagcgcg gtacaggacc gcgatgtgct tcaccagcgc ccgcgcctgc
     8161 tcgagcttcg gcgcgcgcgg cagggggtgg cggccggcgt cgtcgaacag gatcagctcg
     8221 acctcctgcg cgccgatcgc ctcctgcgcg aggtttgcga ccagcagcgg cagctttttc
     8281 ttcttgcgct tgtcctgggc gaaccggtcg aggtcgtggc tctccgcggc gaagccgacg
     8341 cagaagggcg ggtccgggcg cgtggacacc gaggcgagga tgtccacggt gggctccaac
     8401 tcgagcgtga gcggccgatc gttcttcttg attttctgca ccgacgcttg cttcacccgg
     8461 tagtcggcca cggcggctac cgcgacgaat acgtcggtgg cggcgatctc ggcactcacc
     8521 gcctggagca tgtcctgtgc cgaggccacc ggcaccacgc gggcgcccgc gggcgcggtg
     8581 agagccgtgg gaccggacac gagcgtgacc tgcgcaccgg cctccagggc cgcctgcgcg
     8641 accgcatagc ccatgcgccc cgagctgcgg ttggtgaggc cgcgcaccgg gtcgatggct
     8701 tcgaaggtcg ggccggcggt caccagcacg cgcacgccgg ccagcagctt gggctgcagc
     8761 gcggcgacga tggccgcgtg caggtccgct gcttccagca tgcggcccat gccgacctcg
     8821 ccgcaggcct ggtcgccgct gcccggcccc aggatcacca cgccgtccgc gcgcagccgc
     8881 tctacgttgc gctgggtggc cgcgttctcc cacatctggc gattcatcgc cggcgctacc
     8941 agcagcgggc attcgcgcgc caagcacaac gtagacagaa gatcgtccgc cagaccgccg
     9001 gcgagcttgc cgaggaagtc cgcgctggcc ggcgcgacca tgatcaggtc gcggtcgcgc
     9061 gagagctcga tatgccccat cgcgttgggg atcgaggcgt cccacagatc ggtcagcacc
     9121 ggctgtccgg acagggcctg catcgtcacg ggcgcgacga aacggcaggc ggcgtcggtc
     9181 atgaccacgc gcacgtccat gccgtcgcgg accagcaggc ggcacagctc ggcggccttg
     9241 taggcggcca cgccgccggt gatgcccaga aggatgcggt gctgcgggag cgctgtcatg
     9301 ggtctccaag gggccgtcgg caacgggggg tcgtccacaa cggggggcgt ccacaacgtc
     9361 aagtgtatta tacggctcgc aaatcacgcg ccagaccgat gggcatcgcc gattggccgc
     9421 aggaccgcca gccccgggag aagctgctcg cccggggcga ggccgcgctc agcgagccgg
     9481 agctgctggc catcgtcctg cgtaccggcg tgcgcgggaa gagtgccgtc gaccttgcgc
     9541 gcgagctgct cgagcgcttc ggcaccctgc acgggctgct cgaggccgag cccgaggcga
     9601 tccagcgctc gagcggcctg gggccggcca agtccgcgca gctgcgcgcg gtgctggcgc
     9661 tggtcaagcg tgccctcggg caggaggtgc agcgccgcga caacctcagc tcgccgcagg
     9721 cggtgcgcga ctacctgcgg ctcaatctgg cgcagcgcga ccgcgaggtc ttcgtcgccg
     9781 tctttctcga cgcacagaac cgtgtgctca agatccagga gctgttcgcc ggcaccctca
     9841 cgcagaccag cgtgttcccg cgcgacgtgg tgcgctacgc gctgctcgcc aacgccgccg
     9901 ccgtgatctt cgcgcataat catccctccg gcctggccga gcctagccgc gccgacgaga
     9961 cgctgacgca ggcgctcaag caggcgctgg cgctggtgga cgtgaaggtg ctggatcatt
    10021 tcgtcgtcgg cgcgggcggt gcgatgtcct tcgccgagcg cggactgctg tagcccgggc
    10081 ggccgcacct atcgcgggcc gcgctcgtcc tgtgcggccg tctgatccgc agggccggcg
    10141 acggcctgga tcagcgacag caggttgtcg atctggtagt tggcgatcgt gatgacgcta
    10201 gaactgccga ctgcctgcac atagcggctg aactgatccg gtgcaagatc gccgaccgcg
    10261 tactggacca gcggcagggg ctctttcggc cggtaggcca ggatcagcag ctcgggcgag
    10321 gccacgccgt aatcgttcac gcgggcaccg gcctgaagct cccgctcgat gcgggctcga
    10381 cccagcgcgg cgaatgcctg accgatgcgc gttgccgccg ccgggtcggt ctgatgctcg
    10441 tgggccgcct ccgaggccgt gtgcacgccg tggtagaacc aattgccggc ggcgtcgcgc
    10501 tcgaagcggt gcagcgtccc ggtgtgcgcc agctcgatgg cgccgagctg g
//
LOCUS       DASPGE010000107         9405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_618205, whole genome shotgun sequence.
ACCESSION   DASPGE010000107 DASPGE010000000
VERSION     DASPGE010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9405
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618205"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VD791_03415"
     CDS             <1..435
                     /locus_tag="VD791_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HXV07050.1"
                     /translation="NLMTRRKFGLALVLMALAMIGDAAWFGAGFAQEAAHQAGGHDFL
                     AQFSIFVLAVFVGYYVVWSVTPALHTPLMSVTNAISSVIVVGALLAVAVETAGVADSD
                     GHFIAKLLGLAALTIQGRGKTQCTSRLRYTTPPRRLRRHPAS"
     gene            488..1885
                     /locus_tag="VD791_03420"
     CDS             488..1885
                     /locus_tag="VD791_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HXV07051.1"
                     /translation="MTANYVALLYLVASVLFILALKGLSSPETARRGNLFGIAGMLIA
                     VFTTLALTRSVATIVAAVAVGGTVGAVVARRVQMTQMPELVAAMHSLVGMAAVLIAIA
                     VVNNPAAFNIAVPMPRGNRLELFIGTFVGAITFSGSVIAFGKLAGLGKHFRLFSSAPV
                     VFRGQHLLNLTLAAAMLGFGVAFFLAGPEGQWLPFGAMAAIAFVLGVLIIIPIGGADM
                     PVVISMLNSYSGWAAAGIGFSLNNTMLIIAGSLVGSSGAILSYIMCRAMNRSFFSVIL
                     GGFGAAEGAAAQGAQQKSVRSGSAEDAAFLMSNAESVIIVPGYGLAVARAQHAVKEMA
                     EKLAGKGVQVRYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMEDINSEFGNTDVVLVL
                     GANDVVNPLAEQPGSPIYGMPILQAHKARTVLVNKRSMASGYAGLDNPLFYLDKTMMV
                     FGDAKKVVEDMVKAL"
     gene            2150..5314
                     /locus_tag="VD791_03425"
     CDS             2150..5314
                     /locus_tag="VD791_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXV07052.1"
                     /translation="MNRNAVFSKTGKGLLEIKNKANRLSKDQYRVLNLVDGKANLVDL
                     ADKAKVSEIEVRKILTGLADGGYVKEFSNPAGDYAGSTVPPSTSYADDLDFTAILGPS
                     QSAKPGFYQSAALEQRQREEAERKASEAAATRAREESERRAKEEADNRARAEAAARKV
                     REDAERRAREEFERQERQVDDIRSKAENERKAREEAERRVREAKEAAVRKLEQDKLDA
                     ERRAKEEAERRVKIQAEARARVEADKRTREEAERKAREDEERRRREEEERKRREEEER
                     KRREEEERRRREEEERRRREEEERRRLEEEQRKRREEEERRRVEEEKRRREEEERKRR
                     EEEERKRREEEERRRREEEEERRRKEEAARRLQEEQERLKRDLEEKQRREQEELLRRM
                     EDERLRLDDSDYRQQAEEQEREEQERVRVDQRRQAEEQRRREEEELRRRREEDERRKA
                     EEEKRRREQEERRRKEETARKPKEVAFPSLDLTDVPIPVPTISSGFDTDLEALKRAEV
                     EVEKEFKVKEDAIRQALEEQERRFRMEEEARAAMDRAEREARERADREARDLALAAER
                     ARRDAEQKAREEAGRRALEEKERRSKEKQDRQKQAEEDRKKREQQRREAEARAREEEL
                     VRRRKDQQEQDRRKADLDRLQVQARRRAFGPGKMAAAGLLVLVLLVVAVVEFAPMSTY
                     APSIEKAASEAVGEPVRVGGVRASLFPSFHVQLDNVTVGNAQDVRVGKVVAYMGFGSA
                     FGEQKDIGKLVLEDVSAPQDALDRTARWLSAEGKPARNISIERLLLNAVRIDMKGNTL
                     PSFDADLLLAPDRSVRAATIETNDGRFTAELTPREAVVDVTARGRNFTPPLGPGFELT
                     DFTAKGQISGTRLRLTELEYSLYGGQGKGSASVSWGGIWTVEGDFTVQRVELEPAMKA
                     LKIDIVSDGTLEAKGRYVLQSAALERLLDGARVDAVFMVRKGNLSGLDLVRALQSPSR
                     DGTAGGKTKFEELSGSLSASGGRYLYNGVRLVAGALTATGQGEIAPDQDVNGRTYVEL
                     RSSANVIRGNFRVQGSLKGMVLRP"
     gene            complement(5391..5966)
                     /locus_tag="VD791_03430"
     CDS             complement(5391..5966)
                     /locus_tag="VD791_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXV07053.1"
                     /translation="MTGGILLAGGAGSRFGGRKLLYPLPDGTPIGVAAWRNLRTALAG
                     SVAVVRAGDDELATLFAGAGAPVVRCEDAHLGMGHSLVCGVRALEHAGGWVVALADMP
                     RVHPQTIAAVAERLRAGARIALPVWNGRRGHPVGFAAVCRDELLALSGDSGARALLER
                     HAAEVVRVPVDDPGIVEDVDTREDAARTRDS"
     gene            complement(5990..6286)
                     /locus_tag="VD791_03435"
     CDS             complement(5990..6286)
                     /locus_tag="VD791_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07054.1"
                     /translation="MEQLRLGLPPRDAHSPLSRTLFGSLTAVLGLCAIALVVLVLLPL
                     LGIIVSAAVGGVILALAGIVMMVPFILVAGTVLAFMARANTRKPSTFRTRPHWR"
     gene            complement(6504..7883)
                     /locus_tag="VD791_03440"
     CDS             complement(6504..7883)
                     /locus_tag="VD791_03440"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXV07055.1"
                     /translation="MRGRTPARARATPYRVLWALWILLLLCLLPVVALAGVEVSAARV
                     WPAPEYTRITLESARPIEYSLFTLKDPQRLVLDLEDVELGAALESLPASIGADDAFIA
                     QARVGRFKPGVLRMVLEVRGDVRPQAFALRPAGEYGHRLVLDVYPAQPVDPLLALLEQ
                     LEPDRSSSAPADPTPGGRVTPAGQSPPEPSTQPAVARASEQAKPRPQGPKARKRNEPQ
                     VVRLLTVAIDAGHGGEDPGAKGRHGTYEKNVTLAIARRLKALVDAEPNMRGVLIRDGD
                     YYVALNDRVSKARRVRADLFVSIHADAFVKPHARGSSVFALSKRGATSAAARWLAQKE
                     NEADLIGGVKLDVPDPYLRETLLDLSQDGTIHHSIKLGAAVLAELGELNTLHKPRVEQ
                     AGFAVLKAPDIPSILVETAFISNPSEEKKLRDTAYQEKIAKAIFSGIRKYFAANPPTL
                     HDKLAVLRP"
     gene            complement(7880..8383)
                     /gene="tsaE"
                     /locus_tag="VD791_03445"
     CDS             complement(7880..8383)
                     /gene="tsaE"
                     /locus_tag="VD791_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HXV07056.1"
                     /translation="MMQSSGLALRRLPGGEEAVATLSDEAATLALGQRLGLALQPGLK
                     VYLRGDLGAGKTTLVRGALRALGYGGRVKSPTFGLVEVYSVSSLYLYHFDFYRFKNPQ
                     EWRDAGFREEFGGPGVCLVEWPEKAGGLLPVPDLTIALEHDGEGRRARLTSTSERGRQ
                     CLDQITQ"
     gene            8459..>9405
                     /locus_tag="VD791_03450"
     CDS             8459..>9405
                     /locus_tag="VD791_03450"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXV07057.1"
                     /translation="MADNWRSRLITGGVARSPNRAMLRAVGFTDRDFDKPIVGVANGH
                     STLNPCNAGIQPLVDRAVAALKDAGAMPQMFGVPTVTDGIGMGTEGMKYSLVSREVIA
                     DCIETSVNGQMMDGVLVVGGCDKNMPGGMMAIARMNVPAIYVYGGTIKPGYWKGQALT
                     IVSPFEAVGAFAAGKMSKEDFDGIERNACPTVGACGGMYTANTMSSSFEALGMSLLGS
                     SQMASPDPEKADSASESARVLVNAVRKNIRPRDIITRKSIENTIALVMATGGSTNAVL
                     HYLAIASAAEVEWTIDDFERIRQKVPVLCNLKPSGKYVAV"
BASE COUNT         1607 a         3135 c         3386 g         1277 t
ORIGIN      
        1 aacctcatga cgcggcgaaa attcggcttg gcgctcgtct tgatggcgct ggccatgatc
       61 ggcgacgcgg cttggttcgg cgcgggcttc gcgcaagagg cggcgcatca agcaggcggt
      121 cacgacttcc tcgcccaatt ctcgattttc gtgctggcgg tgttcgtcgg ctattacgtg
      181 gtgtggagcg tcacgccggc gttgcacacg ccgctgatgt cggtgaccaa cgccatctcc
      241 tcggtgatcg tggtcggcgc gctgctcgcg gtggcggtcg agaccgccgg cgtcgcggac
      301 tccgacggac acttcatcgc caagctgctc ggtctcgccg cgctgacaat ccaaggcagg
      361 ggaaagacac agtgcacatc tcggctgcga tacaccacac cgccccgccg gcttcgccgg
      421 caccccgcct cgtgacaggc ggggaatgtc tttttaagac gcgctttaaa gcgcatgtgg
      481 tcaagtgatg acggccaact acgtcgccct gctgtatctg gtcgcctcgg tgctgttcat
      541 cctggcgctc aagggcctgt cgagtcccga gaccgcgcgc cgcggcaacc tgttcggcat
      601 cgccggcatg ctgatcgcgg tgttcaccac gctcgcgctg acgcgcagcg tggccacgat
      661 cgtcgccgcc gtcgccgtgg gcggaaccgt cggcgcggtg gtcgccagga gggtgcagat
      721 gacgcagatg ccggagctgg tggcggcgat gcactcgctg gtgggcatgg ccgcggtgct
      781 gatcgccatc gcggtggtca acaacccggc ggcgttcaac atcgcagtgc cgatgccgcg
      841 cggcaatcgc ctggagctgt tcatcggcac cttcgtgggc gccatcacct tctccggctc
      901 ggtgatcgcc ttcggcaagc tggccgggct gggcaagcac ttccggctgt tctccagcgc
      961 cccggtggtg ttcagggggc agcatctgct caatctgacg ctggcggcgg cgatgctggg
     1021 cttcggcgtg gcgttcttcc tggccggccc ggagggacaa tggctgccgt tcggcgcgat
     1081 ggccgcgatc gctttcgtgc tgggcgtgct gatcatcatc cccatcggcg gcgccgacat
     1141 gccggtggtg atctcgatgc tcaacagcta ctccggctgg gcggcggcgg gcatcggctt
     1201 ctcgctcaac aacaccatgt tgatcatcgc cggcagcctg gtcggctcct cgggcgcgat
     1261 cctgtcgtac atcatgtgcc gcgccatgaa ccgctcgttc ttcagcgtca tcctgggcgg
     1321 gttcggcgcg gccgaggggg cggcggcgca gggcgcgcag cagaagagcg tgcgctcggg
     1381 cagcgccgag gacgcggcct tcctgatgtc gaacgccgag tccgtgatca tcgtgcccgg
     1441 ctacggtctc gcggtggcgc gcgcccagca cgcggtgaag gagatggccg agaagctggc
     1501 cgggaaaggc gtgcaggtgc gctacgccat tcatcccgtg gccgggcgca tgccggggca
     1561 catgaacgtg ctgctggccg aggccgaggt gccctacgat caggtgttcg agatggagga
     1621 catcaacagc gagttcggca acaccgatgt ggtgctggtg ctgggcgcga acgacgtggt
     1681 gaacccgctc gccgagcagc ccggcagccc gatctacggg atgcccatcc tgcaggcgca
     1741 caaggcgcgc accgtgctgg tgaacaagcg ctccatggcc tcgggctacg ccggcctgga
     1801 caatccgctg ttctacctgg acaagaccat gatggtgttc ggggacgcca agaaggtggt
     1861 cgaggacatg gtcaaggcgc tctgagcctg ccgagccctt gcccgggtgg ccccgagcca
     1921 gggcattatt cttgctgcgg ggccccatcg ttgccgcgcg gggacagcgg caagcgggag
     1981 tcggccgtgg ccggcgacca gcgggtggcc cccaaaaggg tggccggggt ggcccaccag
     2041 ggtggccccc atagcggtcc gcagcggcgc ccggaggccg tcgctgtgcg gagactaccg
     2101 tcctcgcaat gcaatctcat cgctgacaga agcgagcgca aaccagcaca tgaaccggaa
     2161 cgcggtgttc tccaagacgg gcaagggcct gctggagatc aagaacaagg cgaaccgcct
     2221 gtccaaggac cagtaccgcg tcctcaatct ggtggatggc aaggccaacc tggtcgatct
     2281 cgcggacaag gccaaggtgt ccgagatcga ggtgcgcaag atcctcaccg gcctggccga
     2341 cggcggctac gtcaaggagt tctccaatcc cgccggcgac tacgccggca gcaccgtccc
     2401 gcccagcacc tcgtacgcgg acgatctcga cttcaccgcc atcctcggcc cctcgcagtc
     2461 ggccaagccc ggtttctatc agagcgctgc gctcgagcag cgtcagcgcg aggaagccga
     2521 gcgcaaggcc tccgaggcgg cggccacgcg cgcgcgcgaa gagtcggagc gccgggccaa
     2581 agaggaagcc gacaaccgcg cgcgcgccga agcggccgcg cgcaaggtgc gcgaggacgc
     2641 ggaacggcgc gcgcgcgagg agttcgagcg ccaagaacgc caggtcgacg acatccgcag
     2701 caaggccgag aacgagcgca aggcgcgcga ggaggccgag cgcagggtgc gcgaggccaa
     2761 ggaagcggcg gtgcgcaagc ttgagcagga caagctcgac gccgagcggc gcgccaagga
     2821 ggaggcggag cggcgcgtga agatccaggc cgaggcgcgc gcgcgggtgg aagccgacaa
     2881 gcgcacgcgc gaggaagccg agcgcaaggc gcgcgaggac gaggaacggc gccgccgcga
     2941 ggaagaggag cgcaagcgcc gcgaggaaga ggagcgcaag cggcgcgaag aggaggagcg
     3001 ccggcggcgc gaggaagagg agcgccggcg gcgcgaggaa gaggaacgtc ggcgtctcga
     3061 ggaggagcaa cgcaagcgcc gcgaggagga agagcgccgc agggttgaag aagaaaagcg
     3121 gcgccgcgaa gaggaagagc gcaagcggcg cgaggaagag gaacgcaagc ggcgcgaaga
     3181 ggaagagcgc aggcggcgcg aggaagagga ggagcgccgg cgcaaggaag aggccgcacg
     3241 ccgcctgcag gaggagcagg agcgcctcaa gcgcgacctg gaggagaagc agcggcgcga
     3301 gcaggaggag ctgctgcggc gcatggagga cgagcgcctc cgcctcgatg acagcgacta
     3361 ccgccagcag gccgaggagc aggagcgcga ggaacaggag cgcgtgcggg tggaccagcg
     3421 ccgccaggca gaagagcagc gccgccgcga ggaagaagag ctgcgccgcc gcagggaaga
     3481 ggacgagcgc cgcaaagccg aggaagagaa gcggcgccgc gagcaggagg agcgtcgccg
     3541 caaggaggaa accgcgcgca agcccaagga agtggctttc ccgtcgctcg acctgaccga
     3601 cgtgccgata ccggtgccga cgatatccag cggcttcgac accgatctgg aagcgctgaa
     3661 gcgcgccgag gtcgaggtcg agaaggagtt caaggtcaag gaagacgcga tccgccaggc
     3721 gctcgaagag caggagcggc gcttccgcat ggaggaagag gcgcgcgcgg ccatggaccg
     3781 tgccgagcgc gaggcgcgcg agcgtgccga ccgcgaagcg cgcgacctgg cgctggctgc
     3841 cgagcgcgcg cgccgcgacg cggagcagaa ggcgcgcgag gaggcggggc ggcgcgccct
     3901 ggaagagaaa gagcgccgct cgaaggaaaa gcaagaccgg cagaagcagg ccgaagagga
     3961 tcgcaagaag cgcgagcagc agcggcgcga ggccgaagcg cgcgcacgcg aagaggagct
     4021 ggtgcgccgc cgcaaggacc agcaggagca ggaccggcgc aaggccgatc tcgaccggct
     4081 gcaggttcag gcccgccgcc gtgcgttcgg gcccgggaag atggcggcgg cggggttgct
     4141 ggtgctggtg ctgctggtcg tcgcggtggt cgagttcgcg ccgatgtcga cctatgcgcc
     4201 gagcatcgag aaagcggcca gcgaggcggt cggcgagccg gtgcgcgtgg gcggagtgcg
     4261 cgcctcgttg ttcccctcgt tccatgtcca gctcgacaac gtcaccgtgg gcaacgccca
     4321 ggatgtgcgc gtcggcaagg tagtggccta catgggcttc ggctcggcct tcggcgagca
     4381 gaaggacatc ggcaagctgg tgctggagga cgtcagcgcg ccgcaggatg cgctcgaccg
     4441 cacggcgcgt tggctctccg ccgagggcaa gcccgccagg aacatcagca tcgagcgcct
     4501 gctgctcaac gcggtcagga tcgacatgaa gggcaacacg ctgcccagct tcgatgccga
     4561 cttgctgctc gcgcccgatc gcagcgtgcg cgccgcgacc atcgagacca acgacgggcg
     4621 cttcaccgcc gagctgacgc cgcgcgaggc ggtggtggac gtgaccgcgc gcgggcgcaa
     4681 cttcacgccg ccgctaggcc ccggcttcga gctgaccgac ttcaccgcga aggggcagat
     4741 cagcggcacg cgcctgcgcc tgaccgagct ggagtactcg ctctacggcg gtcagggcaa
     4801 gggcagcgcc agcgtttcat ggggcggcat ctggaccgtg gagggcgact tcacggtgca
     4861 gcgcgtcgag ctcgagccgg ccatgaaggc gctgaagatc gacatcgtct cggacggcac
     4921 gctggaggcc aagggccgct acgtgctgca atcggccgcg ctggagcgcc tgctcgacgg
     4981 cgcgcgcgtg gacgcggtgt tcatggtgcg caagggcaac ctttccggcc tggacctggt
     5041 gcgcgcgctg cagtcgccgt cgcgcgacgg caccgccgga ggcaagacga agttcgagga
     5101 gctgtccggc agcttgagcg cctccggcgg ccgctacctg tacaacggcg tcaggctggt
     5161 cgccggcgcg ttgaccgcga ccgggcaggg cgagatcgct ccggaccagg acgtcaacgg
     5221 ccgcacctac gtcgaactgc gctcgagcgc gaacgtgatc cgcggcaact tccgcgtgca
     5281 gggcagcctg aaggggatgg tgctgagacc gtgattcgat ggaaccgtga ttcgtgatta
     5341 cgtgattcgt aacgaccaat cacgagtcac gaatcactac gaatcacgat tcacgaatca
     5401 cgggttctag cggcgtcctc ccgcgtgtcc acgtcctcga cgatccccgg gtcgtccacc
     5461 ggcacgcgca ccacctccgc ggcgtgacgc tcgagcagcg cgcgcgcgcc gctgtccccg
     5521 ctgagcgcca gcagctcgtc acggcacacg gcggcgaacc ccaccggatg gccgcgccgg
     5581 ccgttccaca ccggcagcgc gatgcgtgcg cccgcccgca ggcgctcggc gacggccgcg
     5641 atcgtctgcg ggtgcacgcg cggcatgtcc gcgagcgcca ccacccagcc gccggcgtgc
     5701 tccagggcgc gcacgccgca caccaggctg tgacccatcc ccaggtgcgc gtcctcgcat
     5761 cgaacgaccg gcgcgccggc gccggcgaac agcgtcgcca gctcgtcgtc gcctgcgcgc
     5821 accaccgcaa cgctgcctgc aagcgccgtg cgtagattgc gccaggcggc cacgccgatg
     5881 ggtgtgccgt cgggcagggg atagaggagc ttgcggccgc cgaagcggga gcccgcgccg
     5941 cccgccagca ggatgccgcc ggtcacggcg gcccccgcgt gccgcgggct cagcgccaat
     6001 gcgggcgcgt gcggaaagtg ctgggcttgc gggtgttggc gcgggccatg aaggccagca
     6061 cggtgcccgc gaccaggatg aaggggacca tcatcacgat cccggccagg gccaggatca
     6121 ccccgcccac ggccgcggac acgatgatgc cgagcagcgg cagcagcacc aggacgacca
     6181 gcgcgatggc gcacaagccg agcacggcgg tcagcgaccc gaacagcgtc cgggacaggg
     6241 gactgtgagc atcccggggc gggagcccga gcctgagttg ttccatggaa gatccttgca
     6301 ctcgcgccgc cgtgggcctt gcggcccggc cattgcgtcc atgctaagcc gaagccggga
     6361 gggctgcaac cggctcgccg cgacagcgca gccaagcaga tcccgcgccc gcctgacccc
     6421 gccgcggtgc aagatttggc ctcgctgacg acgagcaggg tgtgtttctt cttacatcgc
     6481 gccttaaggg cgcaccaggc gccttaaggg cgcagaacgg ccagcttgtc gtgcagggtg
     6541 ggcgggttgg ccgcgaagta cttgcggatg ccggagaaga tggccttggc gatcttctcc
     6601 tggtaggcgg tgtcgcgcag cttcttctcc tcgctcggat tgctgatgaa ggcggtctcg
     6661 accaggatcg aggggatgtc cggggccttg agcaccgcga agccggcctg ctccacgcgc
     6721 ggcttgtgca gcgtgttgag ctcgcctagc tcggccagga cggcggcgcc gagcttgatg
     6781 ctgtgatgga tggtgccgtc ctgcgacagg tcgagcagcg tctcgcgcaa gtaaggatcg
     6841 gggacgtcga gcttcacccc gccgatcaga tcggcctcgt tctccttctg cgccagccag
     6901 cgcgccgcgg ccgaggtggc gccgcgcttg gacagcgcga acaccgagga gccgcgcgcg
     6961 tgcggcttga cgaaggcgtc ggcgtggatc gagacgaaca ggtcggcgcg cacgcggcgc
     7021 gccttgctca cccggtcgtt cagggcgacg taatagtcgc cgtcgcggat cagcacgccg
     7081 cgcatattgg gctccgcgtc caccagcgcc ttcagccggc gcgcgatggc gagcgtgacg
     7141 ttcttctcgt aggtgccgtg acggcccttg gcgccggggt cttccccgcc gtggccggcg
     7201 tcgatggcca cggtcagcag ccgcaccacc tgcggctcgt tgcgcttgcg cgccttgggg
     7261 ccttgcggcc gcggcttggc ctgctcgctg gcgcgcgcca ccgcgggctg cgtggacggc
     7321 tcgggcgggg actgtcccgc gggcgtcacc cgaccgccgg gcgtcgggtc ggccggcgcc
     7381 gacgatgagc ggtcgggctc gagctgctcg agcagcgcca acagcggatc gacgggctgg
     7441 gccggatata cgtcgaggac caggcggtgg ccgtactcgc ctgccggtcg cagcgcgaag
     7501 gcctgcgggc gcacgtcgcc gcgcacctcc agcaccatac gtagcacgcc cggcttgaag
     7561 cgcccgacgc gcgcctgcgc gatgaacgca tcgtcggcgc cgatgcttgc cggcagcgac
     7621 tcgagcgcgg cgccgagctc gacgtcctcg aggtcgagca ccaggcgctg cggatccttg
     7681 agcgtgaaca gcgagtactc gatcggccgc gccgactcga gcgtgatgcg ggtgtactcg
     7741 ggcgccggcc agacccgcgc cgcggagact tccaccccgg cgagcgcgac caccggcagc
     7801 aggcacagca gcagcaggat ccacagcgcc cacagcacgc ggtagggtgt cgcgcgcgcg
     7861 cgagccggtg tgcggcctct cactgcgtga tctgatccag gcactgtctg ccccgttcgc
     7921 tggtgctcgt caggcgcgcc cggcggcctt cgccgtcgtg ctccagcgcg atggtgaggt
     7981 ccggtacggg cagcagcccg ccggcctttt caggccattc cacgaggcat acccccggcc
     8041 ctccgaactc ctcgcgaaag cccgcatcgc gccattcctg tggattcttg aagcgataga
     8101 aatcaaagtg atataagtat aagctcgaaa cgctataaac ttcaaccaag ccgaaggtcg
     8161 ggctcttgac ccggccgccg tatcccaggg cgcgcagcgc cccgcgcacc agggtggtct
     8221 tgcccgcgcc gaggtctccc cgcaggtaga ccttcaggcc cggttgcagt gccagcccca
     8281 ggcgctgccc cagcgccagc gtggcggcct cgtcggaaag cgtggcgacg gcttcttcgc
     8341 cgccgggcag ccggcgcagc gccaagccgc tcgattgcat catgcccgca atactagacg
     8401 cgcgcggcga atctgctcaa acttccccca caccccgcat tcggaggaga caccatcgat
     8461 ggccgacaac tggcgctcca gactcatcac cggcggcgtg gcgcgctcgc cgaaccgcgc
     8521 catgctgcgc gcggtgggct tcaccgaccg cgatttcgac aagcccatcg tcggcgtggc
     8581 caacggccac tccaccttga acccctgtaa cgccggcatc cagccgctgg tggaccgcgc
     8641 cgtggccgcg ctcaaggacg ccggcgccat gccccagatg ttcggcgtgc ccaccgtcac
     8701 cgacggcatc ggcatgggca ccgagggcat gaagtactcc ctggtgtcgc gcgaggtcat
     8761 cgccgactgc atcgagactt cggtcaacgg ccagatgatg gacggcgtgc tggtggtggg
     8821 cggctgcgac aagaacatgc cgggcgggat gatggcgatc gcgcgcatga acgtgccggc
     8881 catctacgtg tacggcggca ccatcaagcc cggctactgg aaaggccagg cgctcaccat
     8941 cgtcagcccg ttcgaggcgg tgggcgcgtt cgccgccggc aagatgtcga aggaggactt
     9001 cgacggcatc gaacgcaacg cctgccccac ggtgggcgcc tgcggcggca tgtacaccgc
     9061 caacaccatg tcctcgtcgt tcgaggccct gggcatgagc ctgctcggct cctcgcagat
     9121 ggcctcgccc gatcccgaga aagcggactc cgcgtccgag tcggcgcgcg tgctggtcaa
     9181 cgccgtgcgc aagaacatcc gcccgcgcga catcatcacg cgcaagtcga tcgagaacac
     9241 catcgcgctg gtgatggcca ccggcggctc gaccaacgcg gtgctgcact acctcgccat
     9301 cgcttcggcc gccgaggtgg aatggaccat cgacgacttc gagcgcatcc gccagaaggt
     9361 gccggtgctg tgcaacctga agccctcggg caagtacgtg gcagt
//
LOCUS       DASPGE010000108         2701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1437244, whole genome shotgun sequence.
ACCESSION   DASPGE010000108 DASPGE010000000
VERSION     DASPGE010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437244"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /gene="mtnA"
                     /locus_tag="VD791_03455"
     CDS             <1..789
                     /gene="mtnA"
                     /locus_tag="VD791_03455"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HXV07058.1"
                     /translation="LAASRPTAVNLAWALARMRRRLDALAGRTPPDIAGALLDEAHAI
                     AAEDVEINRRMGAIGAALITDGARVLTHCNAGALATAGHGTALGVIRSAVAAGKRVSV
                     IADETRPFLQGARLTAWELLQENIPVTLVADAGAGHLMSRGEVDLVVVGADRVAANGD
                     TANKVGTYTLAVLAQRHRVPFYVVCPLSTIDLSTASGGDIPIEERPAEEVTGFRETRW
                     APAGVAVRNPAFDVTPAELITAIVTERGIVRPVTASGLRALFAA"
     gene            complement(786..1526)
                     /locus_tag="VD791_03460"
     CDS             complement(786..1526)
                     /locus_tag="VD791_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HXV07059.1"
                     /translation="MRLAGKVSIITGAARGIGHATALKFAREGALVVVCDLERDGVDA
                     TVGEIREANGEALGFVVDVTKPGSIRAMVDAVMQKYGRIDVLVNNAGIVQDAQLTKMS
                     EVQFDRVIDVNLKGTYNCAKAVADIMVAQGSGVILNASSIVGVYGNFGQTNYAASKFG
                     VIGLVKTWARELGRKGVRANAVCPGFVETPILKSIPAKVLEAIKARVPMGRLARPEEI
                     ANTYAFLASDEASYINGAVIEVSGGATI"
     gene            complement(1600..2088)
                     /locus_tag="VD791_03465"
     CDS             complement(1600..2088)
                     /locus_tag="VD791_03465"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXV07060.1"
                     /translation="MKRIRIRLDAQTLELHDDADGSIVRYLVSTSGRGAGERNGSYCT
                     PRGRHVIRAKIGAGAPLNAVFVERRPTGETYSPELATRFPQRDWILTRILWLSGCEPG
                     FNRLGEVDTMRRMIYIHGSPDSAVMGKPGSIGCVRMHNADLVDLFERVPVGTPVDIVE
                     RE"
     gene            complement(2085..2567)
                     /gene="tadA"
                     /locus_tag="VD791_03470"
     CDS             complement(2085..2567)
                     /gene="tadA"
                     /locus_tag="VD791_03470"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HXV07061.1"
                     /translation="MTEGAPPDRADREFMRHALRLAERAREAGEVPVGAVLVLEGEVA
                     GEGYNAPISAADPSAHAEIRALRDAAGRTGNYRLPGSTLYVTLEPCAMCVGAMFHARV
                     ARVVFGAGDPKTGAAGGVIDLFAEPCLNHHAQVQGGVLAEECGALLREFFVNKRNGTA
                     "
BASE COUNT          371 a         1002 c          909 g          419 t
ORIGIN      
        1 ctcgccgcga gccgccccac cgctgtcaac ctggcctggg cgctggcgcg catgcggcgc
       61 cggctggacg cgctggccgg ccgcacgcca ccggacatcg ccggtgcctt gctggacgag
      121 gcccatgcga tcgccgccga agacgtggag atcaaccgtc gcatgggggc gatcggcgcg
      181 gcgctgatca ccgacggcgc acgcgtcttg acccattgca acgcaggcgc actggccacc
      241 gccggtcacg gcaccgcgct cggcgtgatc cgctcggcgg tcgccgccgg caagcgcgtg
      301 agcgtgatcg ccgacgagac gcgtccgttc ctgcagggcg cgcgcctgac cgcctgggag
      361 ctgctacagg agaacatccc ggtcacgctg gtggcggacg ccggcgccgg gcacctgatg
      421 agccgcggcg aggtggatct ggtggtggtg ggcgccgatc gcgtcgcggc caacggcgac
      481 accgcgaaca aggtcgggac ctacaccctg gcggtgctgg cgcaacgcca ccgggtgccg
      541 ttctacgtgg tctgcccgct ttcgacgatc gacctgtcca ccgccagcgg cggcgacatt
      601 ccgatcgagg agcgtcccgc cgaggaggtg accggttttc gcgaaacgcg ctgggcgccg
      661 gcgggcgtgg cggtgcgcaa tcccgcattc gacgtcaccc cggcggagct gatcaccgcc
      721 atcgtcaccg agcgcggcat cgtacgcccg gtgaccgcgt ccggcctgcg cgcgctgttc
      781 gccgcctaga tcgtcgcgcc gccgctcacc tcgatcaccg cgccgttgat gtagctcgcc
      841 tcgtcggagg cgaggaaggc ataggtgttg gcgatctcct cgggcctggc gagccgcccc
      901 atcggtacgc gcgccttgat cgcctcgagc accttcgcgg ggatgctctt caggatgggc
      961 gtctccacga aacccgggca cacggcgttg gcgcgcacac ccttgcggcc cagctcgcgc
     1021 gcccaggtct tgaccagccc gatgacgccg aacttgctgg ccgcgtagtt ggtctggccg
     1081 aagttgccgt agacgccgac gatggaggag gcgttgagga tcacgccgga gccttgcgcc
     1141 accatgatgt cggccaccgc cttggcgcag ttgtaggtgc ccttcaggtt gacgtcgatc
     1201 acgcggtcga actgcacctc gctcatcttc gtgagctgcg cgtcctgcac gatgccggcg
     1261 ttgttcacca gcacgtcgat gcggccgtac ttctgcatca ccgcgtccac catggcgcgg
     1321 atcgagccgg gcttggtcac gtccaccacg aagccgagcg cctcgccgtt cgcctcccgg
     1381 atctccccga cggtggcgtc caccccatcc cgctccaggt cgcaaaccac gaccagcgcg
     1441 ccttcgcggg cgaacttcag cgccgtcgcg tggccgatgc cgcgcgccgc gcccgtgatg
     1501 atcgaaacct tgcctgccag ccgcatatgc cttcgtcctt gaacccgggt cgagaaatgg
     1561 ccggcactct acctcagatt accgcgccgc acaatccgct cactcccgct cgacgatgtc
     1621 caccggcgtg ccgaccggca cgcgctcgaa caggtccacc aggtcggcgt tgtgcatccg
     1681 cacacagccg atcgagccgg gcttgcccat caccgcgctg tccgggctgc cgtggatgta
     1741 gatcatgcgc cgcatggtgt ccacctcccc cagccggttg aagcccggct cgcagcccga
     1801 cagccacagg atgcgggtga ggatccagtc gcgctgcgga aagcgcgtcg ccagctcggg
     1861 cgagtaggtc tcgccggtcg gccggcgctc cacgaacacg gcgttgagcg gcgcgccggc
     1921 gccgatcttg gcgcggatca cgtggcggcc gcgcggggtg cagtagctgc cgttgcgctc
     1981 ccccgcgccg cgcccggaag tcgagaccag atagcgcacg atcgaaccgt ccgcgtcgtc
     2041 gtgcagctcc agcgtctggg cgtccagccg gatgcggatg cgcttcacgc ggtgccgttt
     2101 cgtttgttta cgaagaactc gcgcagcagg gcaccgcact cctcggcgag cacgccgccc
     2161 tgcacctgcg cgtgatggtt caggcacggc tccgcgaaca ggtcgatcac gccgcccgcc
     2221 gcgccggtct tcgggtcgcc ggcgccgaac accacgcgcg ccacgcgcgc gtggaacatc
     2281 gcgccgacgc acatcgcgca cggctcgagc gtgacgtaca gcgtgctgcc gggcagccgg
     2341 tagttcccgg tgcggccggc ggcgtcgcgc agcgcgcgga tctcggcgtg cgcgctggga
     2401 tcggcggcgg agatgggcgc gttgtagccc tcgccggcca cctcgccttc gagcaccagc
     2461 acggcaccca ccggcacctc gccggcctcg cgtgcccgct cggccaggcg cagcgcgtgg
     2521 cgcatgaact cgcggtcggc ccgatccggc ggcgcgcctt cggtcattcc cactcggcgg
     2581 tcattcccac tcggcggtca ttcccactcg gcggtcgttc ccactcggcg gtcgttccca
     2641 ctcggcggtt attcccaccc gggggctatt cccactcgat ggtggcgggc ggcttgcccg
     2701 a
//
LOCUS       DASPGE010000109        10328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1204039, whole genome shotgun sequence.
ACCESSION   DASPGE010000109 DASPGE010000000
VERSION     DASPGE010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10328
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204039"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /gene="galU"
                     /locus_tag="VD791_03475"
     CDS             complement(<1..725)
                     /gene="galU"
                     /locus_tag="VD791_03475"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HXV07062.1"
                     /translation="MGTRFLPATKASPKEMLPIVDKPLIQHAVEEAVAAGITHMVFVT
                     GRNKRAIEDHFDKAYELETELTGRGRHKLLEHVQDIVPKEVTCSYVRQSVALGLGHAV
                     LCAKAVVHGEPFAVILADDLIDAPLPALKQMVDVYEHRHRSVIAVQNVPREETANYGI
                     IQGEPDGDRLLRMTGIVEKPRPERAPSTLAVVGRYIFGPAIMDHLERVRPGAGGEIQL
                     TDAIASLLHEETVLGYTFDGTRY"
     gene            complement(775..2913)
                     /gene="ligA"
                     /locus_tag="VD791_03480"
     CDS             complement(775..2913)
                     /gene="ligA"
                     /locus_tag="VD791_03480"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HXV07063.1"
                     /translation="MKVPAQARERATRLRHEIERHNYLYYVLDAPEISDAEFDRLFRE
                     LEALEARYPELRTADSPTQRVGAPVQGELAPVVHAVPMLSLNNALTEEEAAAFDRRVC
                     DALGMQEVEYAAEPKFDGLAVSLAYADGALAVGATRGDGYTGEDVTPNLRTIRAVPLR
                     LAADRPPALLEVRGEVVMLKKDFAAINAQQRARGEKEFVNPRNAAAGALRQLDPRITA
                     SRPLHFFAYAISRFEGPLEAQPEAVQRAPAARPKPAGRAGERQPPGLPQDRHSRQMEY
                     LQALHLPVSPRRRVVKGLQGMLDYYREIGRERASLPYDIDGVVYKIDDLKLQQRLGFV
                     SRAPRFAVAHKFPAEEAVTEVLDIVVQVGRTGALTPVARLKPVFVGGVTVTNATLHNE
                     DEVRRKDVHIGDFVVVRRAGDVIPEVVRVVPEKRRRQLRAFKLPERCPVCHSRVRRLP
                     DEAVARCTAGLFCPAQRKQALLHFASRRAMDIDGLGEKIVDQLVDGKRVQTPADLYRL
                     RVEDLAALERMGEKSAANLVAAIDRSRRTTLARFVYALGIRNVGESTARDLARSFRGI
                     EELMHADVERLQRVPDVGPVVAQSIADFFAERHNREVIAQLLAGGVRPEAGGGRPDAA
                     GGTGPSGGRLAGKSFVLTGTLPSMTREEASAAIESAGGRVSGSVSKKTDYLVAGADPG
                     SKYDKALALGVPVLDQAGLLELLESGPEAG"
     gene            complement(2920..4155)
                     /locus_tag="VD791_03485"
     CDS             complement(2920..4155)
                     /locus_tag="VD791_03485"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HXV07064.1"
                     /translation="MGELLIWLIVLGVVAVVAVYVYNRMEEARFRKRTESAFAGQDGD
                     ALMDDELAPAAPGERIEPQLQADASAEAPGPADRREPRAMSEPAPRSGPLPTPAAAIE
                     ALKPPAGAVRPAPDAPAGHAASTAAAARKPSAPQRQPADAALDPIAYSAEIYAREAIP
                     SAALEQMLRAIGPLVARVRLEGRPDAGTPWTALDRTAYGASPHVRASLQLVDRRGAIG
                     KQDLITFQSAVARCAASVAASASIPESEPFLSRARELDAFCAEVDVAVGINIVAPPGR
                     PFLGTRLRALAEAAGLRLADGAFVYPDNRGGVRFSLENQEQTRLLGEALRNLQTTGVT
                     LVLDVPRLADGVSAFDEMVAVGRKLAVSLGGTLVDDNNVPVTDTGLEQIRAQLRAIYG
                     GMQARGIPAGSPAALRLFS"
     gene            complement(4171..7689)
                     /gene="smc"
                     /locus_tag="VD791_03490"
     CDS             complement(4171..7689)
                     /gene="smc"
                     /locus_tag="VD791_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004527298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HXV07065.1"
                     /translation="MRLSHIKLAGFKSFVDPTHIPVPGRLVGVVGPNGCGKSNVIDAV
                     RWVLGESSARHLRGETMQDVIFNGSGERKPANRASVELVFDNALGKAGGPWAKYAEIS
                     VKRVLERDGESAYFVNNMHVRRRDVADIFLGTGLGGRAYAIIEQGMISRIVEARPEEL
                     RTFLEEAAGISKYRERRKETEQRLEDTRENLARVDDILQELAKQLAHLEEQAEVAGRY
                     RALEANLKTTQQLLWLLRKQEAANSRGRVTRELEQVGLELEAETARLRELESRVETLR
                     VDHYGASDAVHGAQGALYEANAETARLEQALQHLRDSRRRVDHQVVALSREREETTQQ
                     LQALRAQLAEQRDALEAAGGHLDACREQERQEAQLLPAAEQAFAATRAEAERVAEDLT
                     RHRQEEQIELTRRGHAAKVLAQLAQRTERLQQERAVLEDVDREELERLTAALHELDWQ
                     YRHKRDTLAKAEALLPDLEQAHRESADAADAAGRELGALDARKTALELLQKQVSRSGE
                     MHAWLDDHQLDGLGRLWQGVRIEPGWEDALEAVLRERLNGIALEDLQRASAWLQHPPP
                     GKVSFFQKQASGPAPASTGARTALSSYVACTDPGVAAVLADWLEGVYVAADPAEGFTR
                     SGELAAGEVLVCPQGHVFTRNSVNFHAADSEIHGILSRQREIEDLERRRQGLQMNLAA
                     TRSRVVETQAELEHGRAELHKLRQDAEAAQQQRHDLQLDQVRLSQQAERVAHRRAQIE
                     AELEEIATEHQREIGVQERARRHLEELALRLEEMERQLEAARAAHDEAGRALDQRRDG
                     AQSAARRLQEAVFNQRAIGQRIDDAESGIQGLAEKLERVEAALREQRQELAGLDEAPL
                     QTQLGEALGQRLEREQALNRARGALEESERVLKEAEQQRMAAEQKLGPLRERSGELRL
                     KEQEARIAEENFAAQLAEVHADESELAGKLEKGMRPNALQSEINRLGQEIAALGAVNL
                     AALEELAAARERKAYLESQSRDLHEALQTLENAIRRIDRETRERLQTTFDEVNRNLAQ
                     LFPTLFGGGEARLTMTGEEILDAGISIVARPPGKRNTSIHLLSGGEKALTALALVFSL
                     FQLNPAPFCLLDEVDAPLDDSNTERFCHLVQQMTQNTQFVFISHNKITMELAEQLVGI
                     TMPEQGVSKVVAVDIEEALKIREEAA"
     gene            complement(7893..8765)
                     /locus_tag="VD791_03495"
     CDS             complement(7893..8765)
                     /locus_tag="VD791_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXV07066.1"
                     /translation="MTEGKTTAVLGLLTGALVWGLIWYPYRALEQVGVSGSLATLASY
                     GVGLLAAVALFRRDLGPLLRPDPTLALIALSAGWCNLAYVLGMIHGQVMQVLLLFYLA
                     PLWTVLFARLLLDERPGAVGAAVILLSLTGAAFMLWQPGARLPLPRSGAEWLGLSSGL
                     TFALSNVLIRRARHQSIRLKSAAVLLGVVTVAALVVPFEAGAALPRPAGVAWPHLALL
                     ILLGLVLLATNLAVQHGLTLTSANRAIVIMLTELVVGAASAYLLAGEAMRWNEWAGGA
                     MIVAASLLSGRMRT"
     gene            8811..10007
                     /gene="dapC"
                     /locus_tag="VD791_03500"
     CDS             8811..10007
                     /gene="dapC"
                     /locus_tag="VD791_03500"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HXV07067.1"
                     /translation="MNPHLARLQQYPFQKLAALLKGVHPDPALSPINLSIGEPRHATP
                     EFIKRALVDNLDGLAGYPPTLGTEALRASIAAWITRRYGLPAVDPARQVLPLNGSREG
                     LFAIAQAVVDNTRAKARVVMPNPFYQIYEGATLLAGAEPEFINTLPGDGYRLDFDQLP
                     ATAWAQTQLVYVCSPGNPTGSVMRMQDWERLFALSDRHGFVIASDECYSEIYFDETDP
                     PLGGMQAAHRLGRDGYPRLICFGSLSKRSNVPGMRSGFVAGDAALLEKFLLFRTYLGN
                     AMSLTVQAASRAAWEDEAHVRDNRRLYREKFEAALAVLRGVVPVSLPQGAFYLWLPTP
                     TEDTEFARRLYQTYNVMVLPGSYLAREARQVNPGRGFVRAALVSAPAECDEAVRRIRA
                     FLTEHA"
     gene            10020..>10328
                     /locus_tag="VD791_03505"
     CDS             10020..>10328
                     /locus_tag="VD791_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HXV07068.1"
                     /translation="MSELQSTIESAFDNRANLTPANAESRLREAVNEVLAQLDAGKLR
                     VAEKRGGEWITHQWIKKAVLLSFRLDDNAVLRDGYTSYYDKVPAKFADYSGRDFRAGG
                     F"
BASE COUNT         1450 a         3777 c         3501 g         1600 t
ORIGIN      
        1 tcgtagcgcg tgccgtcgaa ggtgtagccg agcacggtct cctcgtgcag cagggaggcg
       61 atcgcgtcgg tgagctggat ctcgccgccg gcaccgggcc gcacccgctc cagatggtcc
      121 atgatcgcgg gtccgaagat gtagcgcccg accaccgcca gggtggaagg cgcccgctcc
      181 ggccgcggct tttcgacgat gccggtcatg cgcagcaggc ggtccccgtc gggttcgccc
      241 tggatgatgc cgtagttggc cgtctcctcg cgcgggacgt tctgcaccgc aatcaccgag
      301 cggtggcgat gctcgtacac gtccaccatc tgcttgagcg ccggcagggg agcgtcgatc
      361 aggtcgtccg ccaggatcac cgcgaagggc tcgccgtgca ccaccgcctt ggcgcacagc
      421 accgcatgcc ccaaccccag ggccaccgac tggcgtacat aggagcaggt cacctccttg
      481 ggcacgatgt cctgcacgtg ctctagcagc ttgtggcgcc cgcgcccggt gagctcggtt
      541 tccagctcgt aggccttgtc gaagtggtcc tcgatcgcgc gcttgttgcg cccggtgacg
      601 aacaccatgt gcgtgatgcc ggcggccacc gcttcctcca ccgcatgctg gatgagcggc
      661 ttgtccacga tcgggagcat ctccttgggg ctggccttgg tggcggggag aaaccgcgtc
      721 cccatccccg ccacggggaa tacggccttg gtgaccttca aatcgcctcc tgtctcaccc
      781 ggcttcaggc ccggactcga gcaattctag caatcccgcc tggtcgagca caggcacgcc
      841 caatgcaaga gctttgtcat acttgctgcc cgggtcggcg ccggccacca ggtaatcggt
      901 cttcttggac acgctccccg acaccctgcc ccccgccgac tcgatcgccg cgctggcttc
      961 ctcgcgcgtc atggacggca gcgttccggt gagcacgaag ctcttgccgg ccagccggcc
     1021 gccggagggt ccagtgccgc cggctgcatc cgggcgcccg ccgccggcct cggggcgcac
     1081 accgcccgcc agcaattgtg cgatcacctc gcggttgtgg cgctcggcga agaagtcggc
     1141 gatgctctgc gccaccaccg gtccgacgtc gggcacgcgc tgcaggcgct ccacgtcggc
     1201 gtgcatcagc tcctcgatgc cgcggaacga gcgcgccagg tcgcgtgcgg tggactcgcc
     1261 gacgttgcgg atgcccaatg cgtagacaaa gcgcgccagc gtagtgcgcc ggctgcggtc
     1321 gatcgcggcc accaagttcg ccgccgactt ctcgcccatg cgctccagcg cggccaggtc
     1381 ctcgacacgc agccgataga gatcggcggg cgtctgcacc cgcttgccgt ccaccagctg
     1441 atcgacgatc ttctcgccca ggccgtcgat gtccatggcg cgccgcgagg cgaagtgcag
     1501 caacgcctgc ttgcgctgcg cgggacagaa cagcccggcg gtgcagcgcg ccacggcttc
     1561 gtccggcagg cgccgcaccc gcgaatggca caccgggcag cgctccggca gcttgaaggc
     1621 ccgcaactgc cggcggcgct tctccggcac cacccgcacc acctcgggga tcacgtcgcc
     1681 ggcgcggcgc accaccacga agtcgccgat gtgcacgtcc ttgcgccgca cctcgtcctc
     1741 gttgtgcagc gtggcgttgg tgaccgtcac gcccccgacg aacaccggct tgagccgcgc
     1801 caccggggtc agcgcgccgg tacgccccac ctgcacgacg atatccagca cctcggtgac
     1861 cgcctcctcc gcgggaaact tgtgcgccac cgcgaagcgc ggcgcgcgcg atacgaagcc
     1921 gaggcgttgc tgcagcttga gatcgtcgat cttgtacacc acgccgtcga tgtcgtaagg
     1981 caggctcgcg cgctcgcgcc cgatctcccg atagtagtcg agcatgccct gcagcccctt
     2041 caccacccgg cggcgcggcg aaaccggcag gtgcagggcc tgcaggtatt ccatctggcg
     2101 gctgtgccgg tcctgcggaa ggccgggcgg ttgcctctcg ccggcccgcc ctgccggctt
     2161 cggccgtgcc gcaggcgcgc gttggacggc ctcgggttgc gcctcgagcg gaccctcgaa
     2221 gcgcgagatg gcgtaagcga agaaatgcag cgggcgcgat gcggtgatgc gcgggtcgag
     2281 ctggcgcagc gccccggcgg cggcgttgcg cgggttgacg aactctttct cgccgcgcgc
     2341 gcgctgctgc gcgttgatcg cggcgaaatc cttcttcagc atcaccacct cgccgcgcac
     2401 ctcgagcagc gccggcggcc ggtcggcggc gagccgcagc gggacggcgc ggatggtgcg
     2461 caggttcggg gtcacgtcct cgccggtgta gccgtcgccg cgggtggcgc ctaccgccag
     2521 ggcgccgtcg gcgtaggcga ggctgaccgc cagtccgtcg aacttgggct cggcggcgta
     2581 ctcgacttcc tgcatgccca gcgcgtcgca cacccggcga tcgaacgcgg ccgcctcttc
     2641 ctcggtgagc gcattgttga gcgagagcat cggcactgcg tgcacgaccg gcgccagctc
     2701 gccctgcacc ggcgcgccca ctcgctgcgt cggcgaatcc gcggtgcgca gctcgggata
     2761 gcgcgcctcc agcgcctcca gctcgcggaa caggcggtcg aactcggcgt cgctgatctc
     2821 cggcgcgtcg aggacgtagt acaggtagtt atggcgctcg atctcgtgcc gcagccgcgt
     2881 cgcccgttcg cgcgcctggg ccggcacctt catcgggcgc tacgagaaca ggcgcagcgc
     2941 agccgggctg cccgccggga tgccgcgtgc ctgcatgccg ccgtagatcg cgcgcagctg
     3001 cgcgcggatc tgctccagcc ccgtatcggt gaccggtacg ttgttgtcgt ccaccagcgt
     3061 cccgccgagc gacacggcga gcttgcggcc aaccgccacc atctcgtcga aggcggacac
     3121 cccgtcggcc agacgcggaa cgtccagcac cagcgtgacg ccggtggtct ggaggttgcg
     3181 cagcgcctcg cccaacaggc gcgtctgctc ctgattctct agcgagaagc gcacgccgcc
     3241 gcggttatcc gggtagacga acgcgccgtc ggccagccgc agccctgccg cctccgccag
     3301 ggcgcgcagg cgcgtgccga ggaacgggcg cccgggcggc gcgacgatgt tgatcccgac
     3361 cgcgacatcg acctccgcgc agaaggcatc cagctcgcgc gcccgcgaca ggaacggctc
     3421 ggactcgggg atgctcgcgc tggcagcgac cgaggcggcg cagcgcgcca ccgcggactg
     3481 gaaggtgatg aggtcctgct tgccgatcgc tccgcgccgg tccaccagct gcagcgaagc
     3541 gcgcacgtgc ggactggcgc cgtatgccgt ccgatccagc gccgtccacg gcgtgccggc
     3601 gtccgggcgt ccctccaatc gcacgcgtgc gacgagcggt ccgattgcgc gcagcatctg
     3661 ctccagcgcc gcggagggga tcgcttccct ggcgtagatc tcggcgctgt acgcgattgg
     3721 atccagggcc gcgtcggccg gctggcgctg cggcgccgag ggcttgcgcg cggctgccgc
     3781 cgtgctcgcg gcgtgaccgg caggcgcatc cggcgccgga cggacggccc ccgcgggcgg
     3841 cttgagcgct tcgatcgcgg ccgccggcgt cgggagcggg ccggaccgcg gcgccggctc
     3901 gctcatggcg cgcggttcgc gccggtccgc cggaccgggg gcttcggccg acgcgtccgc
     3961 ctgcagctgc ggctcgatgc gctcgcccgg agcggccggc gccagctcgt cgtccatcag
     4021 ggcgtcgccg tcctgcccgg cgaaggcgct ctcggtgcgc ttgcggaacc gcgcttcctc
     4081 catgcggttg tacacgtaga ccgcgacgac tgcgaccacg cccagaacga tgagccagat
     4141 cagcagctcg cccatggtcg cgtcagtggc ctacgccgct tcttcacgga tcttgagcgc
     4201 ttcctcgatg tccaccgcca ccaccttgga cacgccctgc tccggcatgg tgatgccgac
     4261 cagctgctcg gcgagctcca tggtgatctt gttgtggctg atgaacacga actgggtgtt
     4321 ctgcgtcatc tgctgcacca gatggcagaa gcgctcggtg ttgctgtcgt cgagcggcgc
     4381 atccacctcg tccagcaggc agaacggcgc ggggttgagc tggaacagcg agaacaccag
     4441 cgccagcgcg gtgagcgcct tttccccgcc ggacagcagg tggatcgagg tgttgcgctt
     4501 gcccggaggc cgggcgacga tgctgatgcc ggcgtcgagg atctcctcgc cggtcatcgt
     4561 caggcgcgcc tcgccgccgc cgaacaaggt ggggaagagc tgcgcgaggt tgcggttgac
     4621 ctcgtcgaaa gtcgtctgca ggcgctcgcg ggtctcgcgg tcgatgcggc gaatggcgtt
     4681 ctccagggtc tgcagcgcct cgtgcaggtc gcgcgattgc gactccaggt aggctttgcg
     4741 ctcgcgcgcg gccgccagct cctccagggc ggcgagattc accgccccca gcgcggcgat
     4801 ctcctggccg aggcggttga tctcggattg cagcgcgttg ggccgcatgc ccttctccag
     4861 cttgccggcg agctcgctct cgtcggcgtg cacctcggcg agctgggcgg cgaagttctc
     4921 ttcggcgatg cgcgcctcct gctccttgag gcgcagctcg ccggagcgct cgcgcaacgg
     4981 tcccagcttc tgctccgccg ccatgcgctg ctgctcggcc tccttgagga cgcgctcgga
     5041 ctcctccagc gcgccgcgcg cccggttcag cgcctgctcg cgctccaggc gttgaccgag
     5101 cgcttcgccg agctgcgtct gcagcggcgc ctcgtccaga cccgccagct cctgccgctg
     5161 ctcgcgcagc gcggcttcca cccgctcgag cttttcggcc aagccctgga tgccgctctc
     5221 ggcgtcgtcg atgcgctggc cgatggcgcg ctggttgaaa accgcttcct gcaggcggcg
     5281 cgcggccgac tgcgcgccgt cgcggcgctg gtcgagcgcg cgtccggcct cgtcgtgcgc
     5341 cgcgcgcgcc gcctcgagct gccgctccat ctcctccagg cgcagcgcca gctcctcgag
     5401 gtggcgacgc gcgcgctcct gcaccccgat ctcacgctga tgctccgtcg cgatctcctc
     5461 cagctcggcc tcgatctgcg cgcggcggtg cgccacccgt tcggcctgct gcgacaggcg
     5521 cacctggtcg agctgcaggt catggcgctg ctgctgggcc gcctcggcgt cctggcgcag
     5581 cttgtggagc tcggcgcggc cgtgctccag ctcggcctgc gtctccacca cgcgtgagcg
     5641 ggtggcggcc aggttcatct gcaggccctg ccgccggcgt tcgaggtcct cgatctcgcg
     5701 ctggcgcgac aggatgccgt ggatctcgga gtcggcggcg tggaagttca cgctgttgcg
     5761 cgtgaacaca tggccctggg ggcacaccag cacctcgccg gcggccagct cgccgctgcg
     5821 agtgaagccc tcggccgggt cggccgccac gtacacgccc tccagccagt cggccaacac
     5881 ggcggccacg cccgggtcgg tgcaggcgac gtagctcgac aatgcggtgc gcgcgccggt
     5941 ggatgcgggg gcgggcccac tcgcctgctt ctggaagaac gaaaccttgc cgggcggcgg
     6001 gtgctgcagc caggcgctgg cccgttgcag gtcctccagc gcgatgccgt tgaggcgctc
     6061 gcgcagcacc gcctcgagcg cgtcttccca gcccggctcg atgcgcacgc cctgccacag
     6121 gcgcccgaga ccgtcgagct gatggtcgtc gagccaggcg tgcatctcgc cggagcggct
     6181 gacctgcttc tgcagcagct ccagggcggt cttgcgtgca tccagcgctc ccagctcgcg
     6241 gccggcagcg tcagccgcat cggccgattc ccggtgggcc tgctccagat ccggcagcag
     6301 cgcctcggcc ttagccaggg tgtcgcgctt gtggcggtat tgccagtcca gctcgtgcag
     6361 cgcggcggtg agccgctcga gctcttcacg gtccacgtcc tccagcacag cgcgctcctg
     6421 ctgcaggcgc tcggtgcgct gcgcgagctg cgccagcacc ttggcggcgt gcccgcgccg
     6481 ggtgagctcg atctgctctt cctgccggtg gcgcgtcagg tcctcggcca cgcgctcggc
     6541 ctcggcgcgc gtcgcggcga acgcctgctc cgccgccggc agcagctgcg cttcctggcg
     6601 ctcctgctcg cgacacgcat ccaggtgccc gccggccgct tcgagcgcgt cgcgctgctc
     6661 cgcgagctgt gcgcgcaacg cctgcagctg ctgagtggtc tcctcccgct cgcgcgacag
     6721 cgcgaccacc tggtgatcca cgcggcggcg gctgtcgcgc aggtgctgaa gcgcttgctc
     6781 caggcgtgcg gtctcggcgt tcgcttcgta cagcgcgccc tgcgcaccgt gcaccgcgtc
     6841 gctggcgccg tagtggtcca cgcgcagggt ctcgacgcgg ctctccagct cgcgcaggcg
     6901 cgcggtctcg gcttccagct ccaggccgac ctgctccagc tcacgcgtga cacggccgcg
     6961 actgttggcc gcctcctgct tgcgcagcag ccacagcagc tgctgcgtgg tcttcaggtt
     7021 cgcctccagg gcgcggtagc ggcccgccac ctccgcctgc tcttccaggt gggcgagctg
     7081 cttggccagc tcctgcagga tgtcgtccac ccgcgccagg ttctcgcgcg tgtcctccag
     7141 gcgctgctcg gtttccttgc gtcgctcgcg gtacttggag atgccggcgg cctcctccag
     7201 gaaggtgcgc agctcctccg ggcgtgcctc gacgatgcgc gagatcatgc cctgttcgat
     7261 gatggcgtag gcgcgccccc ccaggccggt gcccaggaag atgtcggcca catcgcgccg
     7321 gcgcacgtgc atgttattga cgaagtacgc cgactcgccg tcgcgctcga gcacgcgctt
     7381 gaccgagatc tcggcgtatt tcgcccaggg gccgccggcc ttgccgagcg cgttgtcgaa
     7441 caccagctcg acgctggcgc ggttggccgg cttgcgctcg cccgagccgt tgaagatcac
     7501 gtcctgcatg gtctcgccgc gcaggtggcg cgcggacgac tcgccgagga cccagcgcac
     7561 cgcatcgatg acgttcgact tgccgcagcc gttcggaccg accacgccta ccaggcggcc
     7621 gggcaccggg atgtgagtgg gatcgacgaa ggacttgaag ccggcgagct tgatgtgcga
     7681 gaggcgcaat tgagccgaac ctgttttcag ccgaggatca cgggaagccg gcgacgctgc
     7741 aagcgcccgg tcgcttgaaa ccaagacgaa agtcacctaa aacaaacgcc cagtcaccgg
     7801 aatgctgccg tgggaccagg cgctgctcga atgttgacat tttaaccgat tcgacgaggc
     7861 ggatgacagg aagccgcgct gccgcgccgc cgctaggtgc gcatgcggcc cgacagcagg
     7921 cttgccgcca cgatcatcgc gccgccggcc cactcgttcc agcgcatcgc ctcgccggcg
     7981 agcagatagg ccgaggcggc gccgaccacc agttcggtga gcatgatgac gatcgcccgg
     8041 ttggccgagg tcagggtgag cccgtgctgg acggccagat tggtggccag cagcaccagg
     8101 ccaagcagga tcagcagcgc caagtgcggc cacgccaccc ctgccggccg gggcaacgcg
     8161 gcccccgcct cgaacggtac gaccaacgcg gcgaccgtca ccacacccag caggacggcc
     8221 gcggacttga ggcggatgct ctgatggcgc gctctgcgga tgagcacgtt ggacagcgcg
     8281 aaggtgaggc ccgaggacag gcccagccat tccgcccccg agcgcggcag cggcagacgc
     8341 gcgcccggct gccacagcat gaacgcggcg ccggtcagcg acaggaggat caccgccgca
     8401 ccgaccgcgc cgggccgctc gtcgagcaac aggcgcgcga acaggaccgt ccacagcggc
     8461 gccaggtaga acagcagcag cacttgcatg acctgcccgt ggatcatgcc gagcacgtag
     8521 gccaggttgc accagcccgc gctcaacgcg atcagcgcca gggtcgggtc gggccgaagc
     8581 agcgggccga ggtcgcggcg aaacagcgcc acggcggcga gcaaacccac gccgtagctg
     8641 gccagcgttg ccagcgagcc ggacacgccc acctgctcca gcgcccggta ggggtaccag
     8701 atcaggcccc acaccagcgc gccggtgagc agccccagca ccgccgtcgt cttgccctcg
     8761 gtcaccggcc gtccacctta tataatcctc cgcctactcc gcgaacctcg atgaatcccc
     8821 atctcgcacg gctgcagcag tatcccttcc agaagctcgc ggcgctgctc aagggcgtgc
     8881 acccggaccc cgcgctgagc cccatcaacc tgtccatcgg tgagccccgg cacgccacgc
     8941 cggaattcat caagcgcgca ctggtggaca acctggacgg gctcgccggc tatccgccga
     9001 cgctcggcac cgaggcgctg cgagcgagca tcgcggcatg gatcacgcgc cgctacggtc
     9061 tgccggcggt cgaccccgcg cgccaggtgc tcccgctcaa cggcagccgt gaaggcctgt
     9121 tcgcgatcgc ccaagcggtg gtggacaaca cgcgcgccaa agcgcgggtg gtcatgccca
     9181 atcccttcta ccagatctac gagggcgcca cgctgctcgc cggggcggag ccggagttta
     9241 tcaataccct gcccggcgac ggttaccgcc tggacttcga tcagctgcct gcaacggcgt
     9301 gggcgcagac tcagctcgtc tacgtctgca gcccgggcaa tcccaccggc agcgtgatgc
     9361 gcatgcagga ctgggagcgc ttgttcgccc tgtccgaccg gcatggcttc gtcatcgcca
     9421 gcgacgagtg ctactcggag atctacttcg acgagacgga tccgccgctc ggcgggatgc
     9481 aggccgcgca ccgcctgggc cgcgacggct atccgcgcct gatctgcttc ggcagcctgt
     9541 ccaaacgctc caacgtgccc ggcatgcgct cgggcttcgt cgccggcgac gcggcgctgc
     9601 tggagaagtt cctgctgttt cgcacctacc tcggcaacgc catgagcctg accgtgcagg
     9661 ccgcgagccg ggcggcctgg gaggacgaag cgcacgtgcg cgacaaccgc cggctgtacc
     9721 gggagaagtt cgaggccgcg ctcgccgtcc tgcgcggtgt ggtgccggtg tccctgccgc
     9781 agggcgcctt ctatctgtgg ttgcccacgc ccacggaaga caccgagttc gctcgccgcc
     9841 tgtaccaaac ttataatgtc atggtcctgc cgggcagcta tctcgcgcgc gaggcgcgcc
     9901 aagtgaaccc cgggcgcggc ttcgtgcgtg cagcgctggt atccgccccg gcggagtgcg
     9961 acgaggcggt gcggcgcatc cgcgcttttc tcaccgaaca cgcctgagcc ggaaacccca
    10021 tgtccgaact gcaatccacc atcgaatccg cgttcgacaa ccgcgcgaac ctgacccctg
    10081 ccaacgccga gtcgcgcctg cgcgaagccg tcaatgaagt gctggcgcag ctcgacgccg
    10141 gcaagctgcg ggtggccgag aagcgcggcg gcgagtggat cacgcaccag tggatcaaga
    10201 aggcggtcct gttgtctttc cgcctcgacg acaacgcggt gctgcgcgac ggttacacca
    10261 gctactacga caaggtgccg gccaagttcg ccgattacag cggcagggat ttccgggcgg
    10321 gcgggttc
//
LOCUS       DASPGE010000110         5592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1672349, whole genome shotgun sequence.
ACCESSION   DASPGE010000110 DASPGE010000000
VERSION     DASPGE010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672349"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..956)
                     /locus_tag="VD791_03510"
     CDS             complement(72..956)
                     /locus_tag="VD791_03510"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HXV07069.1"
                     /translation="MTTPLFESNLRSLRLLHRGKVRDLYEIDAERLLIVQTDRLSAFD
                     VVLPTPVPGKGAVLTALSLFWFRRLAQVIPTHLLEVDPLALVREDEHDQVRGRAMVVR
                     RLKPLPIEAIVRGYLAGSGWKAYRSTGAICGIPLPAGLKEADRLPSAIFTPSTKAPAG
                     THDQNIPFSEAERLLGAERASRVRDVAIALYTQAAEYARERGLIIADTKFEFGTDASG
                     ALVLIDEALTPDSSRFWPADQHRPGTSPPSFDKQFVRDWLEAQHWDKRPPAPALPPDI
                     LARTAAKYREALRMLTGQ"
     gene            complement(1041..2183)
                     /locus_tag="VD791_03515"
     CDS             complement(1041..2183)
                     /locus_tag="VD791_03515"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HXV07070.1"
                     /translation="MLIEPGAFLGLLGGGQLGRMFTMAAQSMGYRVMVLDPGAHSPAG
                     SVADEHVAADYLDPRALEKLAAQCAAVTTEFENVPAEAMRFLAGHCRISPGADSVAVA
                     QDRIREKRFLAAAGLDVAPHKVIGQADDLRDVGHLLPGILKRSRLGYDGKGQVRVTRS
                     AELNAAFEQLGGEPCVLEQMLELELELSCVVARGFDGAMLSWPVAENQHAQGILDVSI
                     VPARASVALAAAAREGALTVARKLDYRGVLCVEFFVVRGGRLVANEIAPRPHNSGHYT
                     IDACVTSQFEQQARVLCGMPLGDTGLHSPAVMVNVLGDLWAAGEPRWEHVLREPRAKL
                     HLYGKHEARPGRKMGHYTVLGESADAALAKALEIRVKLRADAHGKR"
     gene            complement(2221..2718)
                     /gene="purE"
                     /locus_tag="VD791_03520"
     CDS             complement(2221..2718)
                     /gene="purE"
                     /locus_tag="VD791_03520"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HXV07071.1"
                     /translation="MSAPLVGIVMGSDSDWDVMQHAAAQLDQLGVAHEARVLSAHRTP
                     DEMFRYAESAAGRGLRCIIAGAGGAAHLPGMLAAKTVLPVLGVPVPSRHLQGLASLLS
                     IVQMPRGVPVATFAIGEAGAVNAALFAASLLAVSDPALSAKLRDLRSAQEAKVRAMKL
                     PPAKS"
     gene            complement(2715..4142)
                     /gene="pyk"
                     /locus_tag="VD791_03525"
     CDS             complement(2715..4142)
                     /gene="pyk"
                     /locus_tag="VD791_03525"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HXV07072.1"
                     /translation="MRRSTKIVATVGPASSSREVLTRVLAAGVDVVRLNFSHGSAQEH
                     AERAALVREISRRLGRTVGLMVDLQGPKIRIGSFDQGSIVLGKGDRFILDADCKSGDQ
                     ERVGLDYPELVGDVRAGDVLLLDDGRIVLDVREVRSHEIVTGVRHGGVLSDNKGINRQ
                     GGGLTAPALTAKDVQDIGTAAELKADFLAVSFPKSAADMQRARELLDAAGGHALLVAK
                     IERAEAIKALDEIVQASDAIMVARGDLAVEVGDAAVPALQKRMIRLARERNKVAITAT
                     QMMESMISNPVPTRAEVSDVANAVLDGTDAVMLSAETASGKYPAETVEAMARVCLEAE
                     KSSAVTLDRDFLDRVFTRVDQSIAMAALFTAYHLKVKAIAALTQSGSTALWVSRLNSG
                     VPVYALTPEISTRYKLSLFRSVHTLMLQYSGDNRETLLRFAEDKLVEAGLVQAGDLIV
                     FTFGEPIGVPGGTNTMKIVRVGEQR"
     gene            complement(4146..5333)
                     /locus_tag="VD791_03530"
     CDS             complement(4146..5333)
                     /locus_tag="VD791_03530"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HXV07073.1"
                     /translation="MPILKMTDLDLAGKRVLVREDFNVPLEDGVISDDTRIRASLPGI
                     RHALSGNARLMLVSHLGRPTEGQFDEKESLAPVARRLSELLGQDVALVRNWLDGVDVV
                     PGRAALLENCRFNKGEKKNDDALAKRIAALCDIYVNDAFATAHRAEATTHGVAKYAPI
                     ACAGPLLARELEALGKALENPSRPLVAIVGGAKVSTKLTVLAKLSEKVDQLIVGGGIA
                     NTFMLAAGMKIGKSLAEPDLVAEAKKVMDAVQAKGGRVPLPVDVVCAKELSATAAATT
                     KDVAAVADDDLILDVGPNTAAQLAGLLGRAATIVWNGPLGVFEHDQFAGGTRALAQAI
                     AGSRAFSLAGGGETVAALAKFGVTDKIGYVSTAGGAFLEFLEGRKLPAVEILEQRAAG
                     SRT"
BASE COUNT          833 a         1991 c         1916 g          852 t
ORIGIN      
        1 cgggaaggcg accagtccgc cggcgcgcag gacgtccgca gcctgcgcga tctcctctgg
       61 cgcggggagc gtcattgccc ggtcagcatg cgcagcgcct cgcggtattt cgctgcggtg
      121 cgcgccagga tatcgggcgg cagcgccggg gccggcgggc gcttgtccca gtgctgcgcc
      181 tcgagccagt cgcgcacgaa ctgcttgtcg aagctgggcg ggctagtccc gggacggtgc
      241 tggtcggcgg gccagaaacg cgaggaatcg ggcgtgagcg cctcgtcgat cagaaccagc
      301 gcgccgctcg cgtcggtacc gaactcgaac ttggtgtcgg cgatgatcag cccgcgctcg
      361 cgcgcatact cggcggcctg cgtgtacagc gcgatggcga cgtcgcgcac ccgggaggca
      421 cgctcggcgc cgagcaggcg ctcggcctcg gagaacggaa tgttctggtc atgggtcccg
      481 gcgggcgctt tggtggaggg ggtgaagatg gcgctgggca ggcggtccgc ctccttcagc
      541 cccgccggca gcggaatacc gcagatcgcg cccgtgctgc ggtatgcctt ccagcccgag
      601 cccgccagat agccgcgcac gatcgcctcg atcggcaagg gcttcagccg ccgcaccacc
      661 atcgcccgcc cgcgcacctg gtcgtgctcg tcttcgcgca ccagcgcgag cgggtccacc
      721 tccagcagat gcgtggggat cacctgcgcc agccggcgga accagaacag cgacagcgcc
      781 gtgaggaccg cgcccttgcc cggcaccggg gtgggcagga ccacgtcgaa ggcggacagc
      841 cggtcggtct gcacgatcag caggcgctcg gcgtcgatct cgtagaggtc gcgcaccttg
      901 ccgcggtgga gcagccggag cgagcgcaag ttggattcga acagaggtgt ggtcatcggg
      961 agcgggttgc cctcgccgcg gagtcagaac tgccgcgacg gcgttcgacg tgcggtggcg
     1021 ccggtgcggc gggtgaagtt tcagcgcttt ccatgcgcgt cggcccgcag cttcacacgg
     1081 atctccagcg ccttggcgag cgccgcgtcg gcgctctcgc cgagcaccgt atagtgcccc
     1141 attttgcgac cggggcgcgc ctcgtgcttg ccgtacaggt gcagcttcgc gcgcggctcg
     1201 cgcaggacgt gttcccagcg cggttcaccg gcggcccaca ggtcgcccag cacgttgacc
     1261 atcaccgccg ggctgtgcaa gccggtgtcg ccgagcggca tcccgcacag cacgcgcgcc
     1321 tgctgctcga actgggacgt gacgcaggcg tcgatcgtgt agtgaccgct gttgtgcggg
     1381 cgcggcgcga tctcgttggc caccagccgt ccgccgcgca ccacgaaaaa ctccacgcac
     1441 aacacgcccc ggtagtcgag cttgcgcgcc accgtcagcg ccccctcgcg cgcggcggcc
     1501 gccagcgcca cagacgcgcg cgccggcacg atgctcacgt cgaggatgcc ctgcgcgtgc
     1561 tggttctcgg ccacgggcca gctcagcatg gcgccgtcga agccgcgcgc caccacacag
     1621 gacagctcga gctcgagctc cagcatctgc tccagcacgc acggctcgcc gccgagttgc
     1681 tcgaaagcgg cgttcagctc cgcgctgcgc gtgacgcgca cctgcccctt tccgtcatag
     1741 ccgaggcggc tgcgcttgag tatgcccggc agcagatggc cgacatcgcg caagtcatcg
     1801 gcctggccga tcaccttgtg cggcgccacg tcgagcccgg cggcagcgag gaaccgtttt
     1861 tcgcggatcc ggtcctgggc cacggcgacg ctgtcggcgc ccgggctgat gcggcaatgc
     1921 ccggccagga acctcatggc ttcggccggc acgttctcga actcggtggt gacggccgcg
     1981 cactgcgcgg ccagcttctc cagcgcgcgc gggtccaggt aatcggccgc gacgtgctcg
     2041 tcggccacgc tgcccgcagg gctgtgcgcg ccgggatcca gcaccatcac ccggtagccc
     2101 atgctctggg cggccatggt gaacatgcgg cccagctgtc cgccgccgag cagcccgaga
     2161 aacgcgccgg gctcgatcag catggagcgg gagcagcacc ggccgcgccg cgcgcccgaa
     2221 tcatgacttg gcgggcggca gcttcatggc gcgcactttc gcctcctgcg ccgagcgcag
     2281 gtccctcagc ttcgcgctca gcgcgggatc gctcacggcc agcagcgaag cggcgaacag
     2341 cgcggcgttg accgcgccgg cctcaccgat ggcgaaggtg gccaccggca cgccgcgcgg
     2401 catctgcacg atcgacagca gggaggcgag cccctgcaag tggcggctgg ggactggcac
     2461 gcccagcacc ggcagcaccg tcttggcggc cagcatgccg ggcagatgcg cggcgccgcc
     2521 ggcccccgcg atgatgcagc gcagcccgcg gccggcggcg gattccgcgt agcggaacat
     2581 ttcatccggc gtgcgatgcg ccgacagcac gcgcgcttcg tgcgcaacgc ccagctggtc
     2641 gagctgcgcg gccgcatgct gcatcacgtc ccagtcgctg tcgctgccca tgacgatgcc
     2701 caccagcggc gcgctcatcg ctgctctcct accctaacaa tcttcatagt attcgtcccg
     2761 ccggggacgc cgatcggctc gccgaaagtg aagacgatca ggtcgccggc ctgcaccagg
     2821 ccggcctcca ccagcttgtc ttcggcgaag cgcagcagcg tctcgcgatt gtcgcccgag
     2881 tactgcagca tcagcgtgtg caccgagcga aacaacgaga gcttgtagcg cgtcgagatc
     2941 tccggcgtga gcgcgtacac cggcacgccg gaattgaggc gcgacaccca cagcgcggtg
     3001 gagccggact gggtgagcgc ggcgatcgcc ttcaccttga ggtgataggc ggtgaacagc
     3061 gccgccatgg cgatggactg gtccacgcgc gtgaacacgc gatccaggaa gtcgcggtcg
     3121 agggtgaccg cggaggactt ctcggcctcg aggcagacgc gcgccatcgc ctcgaccgtc
     3181 tccgccgggt acttgccgct cgcggtctcg gccgacagca tcacggcgtc ggtgccgtcg
     3241 agcacggcgt tggccacgtc ggagacctcg gcgcgggtag gcaccgggtt ggagatcatc
     3301 gactccatca tctgcgtggc ggtgatcgcc accttgtttc gctcgcgcgc cagccggatc
     3361 atgcgcttct gcaacgccgg caccgcggcg tcgcccacct ccaccgccag gtcgccgcgc
     3421 gcgaccatga tcgcgtcgga ggcctgcacg atctcgtcca gcgccttgat cgcctcggcg
     3481 cgctcgatct tggccaccag cagcgcgtgt ccccccgcgg catcgagcag ctcgcgcgcg
     3541 cgctgcatgt cggccgcgct cttcggaaag gacaccgcca ggaaatcggc cttgagctcg
     3601 gcggccgttc cgatgtcctg cacatccttg gcggtcagcg ccggtgcggt gaggccaccg
     3661 ccctggcgat tgatgccctt gttgtcggac agtacgccgc cgtggcgcac gccggtgacg
     3721 atctcgtgtg aacgcacctc ccggacatcg agcacgatgc ggccgtcgtc gagcagcagg
     3781 acgtcgccgg cgcgcacatc gccgaccagc tcggggtagt cgaggccgac gcgttcctgg
     3841 tcgccggact tgcaatcggc atccaggatg aagcgatcgc ccttgccgag cacgatgctg
     3901 ccttggtcga aactgccgat gcgaatcttg ggaccttgca gatccaccat cagcccgacc
     3961 gtgcgaccga gccggcgcga aatctcgcgt accagcgcgg cccgttcggc gtgctcctgg
     4021 gcgctgccgt gcgagaagtt caggcgcacc acgtccacgc ccgcggcgag gacgcgggtg
     4081 agcacctccc ggctggagct ggccgggccg acggtggcga cgatcttggt gctgcgtcgc
     4141 aaaggctagg tccttgaccc cgcggcgcgc tgctcgagga tctcgaccgc gggcagcttc
     4201 ctgccttcca ggaactccag gaatgcgcca ccggcggtgg acacgtaacc gatcttgtcg
     4261 gtgacgccga acttggccag cgccgccacg gtctcgccgc cgcccgcgag cgaaaaagcg
     4321 cgtgacccgg cgatcgcctg cgccagcgcg cgcgtgccgc cggcgaactg atcgtgctcg
     4381 aagacgccga gcgggccgtt ccagacgatg gtcgccgcgc ggccgagcag cccggcgagc
     4441 tgcgcggcgg tgttcgggcc gacatcgagg atgaggtcgt cgtcggccac cgcagcaacg
     4501 tccttcgtgg tcgccgccgc agtggcggag agctcctttg cgcacaccac atccacgggc
     4561 agcggaaccc gtccaccctt ggcttgcacc gcatccatga ccttcttcgc ctccgccacc
     4621 aagtcgggtt cggccaggga cttgccgatc ttcatgccgg ccgcgagcat gaaggtgttg
     4681 gcgattccac cgccgacgat cagctgatcc accttctcgg acagcttggc cagcacggtg
     4741 agcttggtgg acactttggc cccgccgacg atcgccacca gcggacggct cgggttttcc
     4801 agcgccttgc cgagcgcctc cagctcacgc gccagcagcg ggccggcgca ggcgatcggc
     4861 gcatacttgg ccacgccgtg ggtggtcgcc tcggcgcgat gcgcggtggc gaaggcatcg
     4921 ttcacgtaga tgtcgcacag cgccgcgatc cttttcgcca gcgcatcgtc gttcttcttc
     4981 tcgcccttgt tgaagcggca gttctccagc agtgcggcgc ggccgggcac gacgtcgacg
     5041 ccgtccagcc agttgcgcac cagcgcaacg tcctgaccga gcagctcgga caggcgccgc
     5101 gcgaccgggg ccagcgattc cttctcgtcg aactgaccct cggtcggccg ccccagatgc
     5161 gagaccagca tcagccgcgc gttcccggac agcgcgtgcc ggatgccggg cagcgaggcg
     5221 cggatgcgcg tgtcgtcgct gatgacgccg tcttccagcg gcacgttgaa gtcctcgcgc
     5281 accagcaccc gcttgccggc gagatcgagg tcggtcattt tcaggatggg catgcgggct
     5341 actggaggta cccgacgggc gatgatgctt cacaccacac cgccccggcg cctgcggcgc
     5401 cacgccgcct cgtgagaggc ggggagtgtt ttttaagtcg cgccttaagg gcgcacccgg
     5461 cggggtggca cgcgaagcgt gacggggtgg tgcagacccc cgcgatcatt tcgcgttaac
     5521 caaggctacg gttgtgtcga gcatgcgatt gctgaagccc cactcgttgt ggctccggcc
     5581 gcgcggcaag cc
//
LOCUS       DASPGE010000111        11292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_853005, whole genome shotgun sequence.
ACCESSION   DASPGE010000111 DASPGE010000000
VERSION     DASPGE010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11292
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853005"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VD791_03535"
     CDS             <1..559
                     /locus_tag="VD791_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952388.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HXV07074.1"
                     /translation="VLGRLKICGPDAEELPPGEVGGVYFADGRPFAYHNDPQMTAEAH
                     NAKGWATLGDIGYVDEEGYLYLTDRKAYVIISGGVNIYPQECENLLAMHPGVHDVAVF
                     GVPNDEFGEEVKAVVQPHDMRDAGPALEAELIAFCRQHLSPIKCPRSIDFEAQLPREP
                     TGKLQKRRLRERYWAGLGGNTPRRT"
     gene            complement(517..1602)
                     /gene="hppD"
                     /locus_tag="VD791_03540"
     CDS             complement(517..1602)
                     /gene="hppD"
                     /locus_tag="VD791_03540"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006497422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HXV07075.1"
                     /translation="MPDLWDNPMRTDGFEFVEYAAPDPAALGALFVKMGFRAVARHRQ
                     KDVTLYRQGDVNFIINAEPHSFAQSFARIHGPCVCAIAFRVRDAGAAYKRAIDLGAWG
                     VPSQPGPMELNIPAIKGIGDSLVYLVDRYPGNDGDLSIYDVDFRWLPGEDRRPAGAGL
                     STVDHLTHNVHRGRMREWAEFYERLFNFREMRYFDIEGKLTGLRSKAMTSPCGKIRIP
                     INESSDDRSQIQEYLDAYHGEGIQHIALASRDIYATVEGLRTAGVAFLDTPDVYYDRV
                     DERVPGHGEDLARLRAVRILIDGAPTQGGGLLLQIFTKTVIGPIFFEIIQRKGNEGFG
                     EGNFRALFESIEEDQVRRGVLPPSPAQ"
     gene            complement(1652..2788)
                     /locus_tag="VD791_03545"
     CDS             complement(1652..2788)
                     /locus_tag="VD791_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HXV07076.1"
                     /translation="MLRESVQQFAAAEIAPRAAEIDRSNEFPAHLWRKFGDMGLLGIT
                     VEEEYGGAGMGYLAHILAVEEISRASAAVGLSYGAHSNLCVNQIRRNGDAAQKRKYLP
                     RLISGEHVGALAMSEPNAGSDVVGMRLRAERRGDRYLLNGNKMWITNGPDADVLVVYA
                     KTDPAAGAKGITAFLIEKGFKGFSTAQKLDKLGMRGSNTGELVFQDCEVPEENVLGQV
                     GRGAQILMSGLDFERAVLSGGPLGVMQACMDVVLPYVHQRVQFGQPIGEFQLMQGKLA
                     DMYSTMNACRAYVYAVGRACDAANSPEQVRALRKDAAGAVLYSAEKATWMAGEAIQAL
                     GGNGYINEFPTGRLWRDAKLYEIGAGTSEIRRWLIGRELFEDTK"
     gene            2992..3525
                     /locus_tag="VD791_03550"
     CDS             2992..3525
                     /locus_tag="VD791_03550"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HXV07077.1"
                     /translation="MQSNPYKTPAAKVADQAEQYGEVKILSASGRMGRVRYIGYGVGM
                     TLVMYLVIGLFALLAGVTDLQFLLVAGMIVGGVGMLVVMVMLTIQRCHDFNMSGWMSL
                     LLIVPIVPLLFWIIPGTQGSNRFGNPPPPNSTGAVVLALILPVLFVVGIMAAIAVPAY
                     VDYQQRAAQAEQSQPTQ"
     gene            3744..4802
                     /locus_tag="VD791_03555"
     CDS             3744..4802
                     /locus_tag="VD791_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV07078.1"
                     /translation="MNDPAAFRPAPAGLNPLQFPFAGPPEAGTTLEIAPGMFWLRMPL
                     PFALDHINLWVLRDGDGWTLIDCGLNSARTRELWEAICGSLLATGSVNRVLVTHFHPD
                     HVGLAGWLTQRFGVELWMTEAEFLTTHVNYAQLPGHTKDGTLALFQRHGLDSARAEVM
                     RAHPHVYRRAISEPPAAFRRIMDGDRIDIGEHAWSVVTGYGHAPEHAALYCRELSLLI
                     SGDMVLPKISTNVSVWAEQPEADPLDLFLKSVDRYAQLPGDTLVLPSHGLPFRGLQAR
                     AAALHEHHAARLDEVLAACAQPVSAAQMVPVLFRRALDDHQMSFAMGEAIAHLNHLLY
                     KGRVVRSEQDGILCFAAV"
     gene            4816..6633
                     /gene="phaC"
                     /locus_tag="VD791_03560"
     CDS             4816..6633
                     /gene="phaC"
                     /locus_tag="VD791_03560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HXV07079.1"
                     /translation="MTAPSDSVPPSSPSPPRPEELAREFADIARQSQRLANDFLAHVQ
                     QGKAPLPSDDVGVTNAFVDLAAKLMANPMQLAEAQMRMWHDYARLWQSAMARFLGETP
                     EPVATPAKSDNRFKNEVWENNFLFDYIKQSYLIAAQNIQRTVSQVQGLDPQTARKAQF
                     FTRQFVDALAPTNFVFTNPEVLHATVESGGRNLIDGLHNLLDDLERGGGQLSVSMTDP
                     SAFKLGENVATTPGKVVYQNDLIQLIQYTPSTQTVWKTPLLIIPPWINKYYILDLREK
                     NSFIRWAVAQGHTLFVISWRNPDARFAGKGFDDYMLEGPIAALEAIERATGEKEANVI
                     GYCLGGTLLGCTLAWLAAKQRNPVKSATFFTALIDFTEPGELGVFVDETVLANLEKKM
                     QERGYLEGSEMAATFNLLRANDLIWSFVVNNYLLGKDPFPFDLLFWNSDSTRMPAKMH
                     TFYLRNMYLRNALVQPGGITLAGEPIDLGKIRTPACFVSTIEDHIAPWKSTYLGARRL
                     PGEVKFILGGSGHIAGIVNPPAGGKYHHWTDDTLTGSADEWLSQAQRRDGSWWPEWNA
                     WVERFGGEKVPARAPGDGRLEPIEDAPGSYVKERLDTQR"
     gene            complement(6636..9413)
                     /locus_tag="VD791_03565"
     CDS             complement(6636..9413)
                     /locus_tag="VD791_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXV07080.1"
                     /translation="MTISQRLWLGLGLILALFAAAHYVSVRATHSLDAVLSEAVEDSE
                     ARSAAAYEMQLALDRMTRAVQSVSDVHEPAQRKQLSEAQAGFERALSSYAAVASTDGS
                     RGLHRDVERRYARFKQRAAALIQQAGSHARRLAAYDAHQREGDALLRALPRVWAARES
                     PRPHGSAVETALGRYFQVAGVLTSEPEVADAWPRIDGARRRVDAALASYEAFARTGAE
                     RQWAASVHRWLEQSDRQGRAILAGERTQYRALADLGAQGEALEQLLDERIHPAARTEL
                     NEAVARASATAHDANLLVTRGLLLALALGVLVAAATTRAVRAPLRALVASSRRVAEGD
                     FSLRVPSGGSDELGKLTVAFNEMAERLQATTVSRSYMESVLDSMGEALLVVSHDGAIE
                     TANPAAARLLGYEREQLCGLVFSAVVDGGSERLLEGVQGPLPARFDVRLLGRDGSRVP
                     VTLSAVPMREPGTAPAIVCIAQDLRERIAAEERLNYLARHDPLTTLPNRLLLGERLRS
                     ALSRAERTGRAVALLYLDLDSFKHVNDTLGHQEGDRLLQAMAGRLQGCVRGQDVVARL
                     GGDEFVVIVEDVSEPRAPARVAEKIISVLSAPFELGGIELRMRASVGISLGPAHGNTA
                     EQLLKAADAAMYRAKRTGQGRYEFFSEELTVQAMERLTLENTLRDPRLLEQLVLHYQP
                     QVSIAQARTVGVEALIRWQHPTRGLLGPHHFIPVAEQIGLIHGIGEWVLRTACAQAKA
                     WIDAGHPPMRMAVNISAQQIAGEAIVTHVVSALRETDLDPALLELEVTEGALQTGAEA
                     MEMLGRLKRLGVHLALDDFGTGYSGLSSLKLLPFDRLKIDRAFVRDLETDPSDRALVR
                     AIIAMARSLKLDVLAEGVETEAQLAFLREHRCDEIQGYLVAKPMRAEELRALLPMSAA
                     RTDLRAVRAGM"
     gene            complement(9564..10031)
                     /locus_tag="VD791_03570"
     CDS             complement(9564..10031)
                     /locus_tag="VD791_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07081.1"
                     /translation="MKIRLTIAQRLWIGLGLLLSPLAAAYLFALHMLSTLETQGDAHA
                     PALASAVRTLELLSNGLLAALMLGAAIAAVTSFAVRAPLRRLIASVRGYVDGDLSYRS
                     LSMRGDEVGELRWIFDAMVGRFGSAPRLGQEVAAGPHERVRDDGALAHDDVRE"
     gene            10195..10992
                     /locus_tag="VD791_03575"
     CDS             10195..10992
                     /locus_tag="VD791_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV07082.1"
                     /translation="MKTILITGAAGDIGTRLRRELAGVYQLQLSDIRPVPALAPGEVF
                     ERGDIADLSDMLRIARGVDAILHLGGVSAESDWESILQANIVGCYNVFEAARRNGVKR
                     VLFATSNHAVGFYRRDETIDHRVYPRPDSRYGLSKVFGEQLGSLYADKYGLEVFCMRI
                     GNLTRAPVDKRRLSIWISPRDLAQLVRIGVEHPAIRFEIVYGVSANRRSWYDNSNAER
                     LGYRPQDDSEPYAAEILAKHVDAADPRVELYQGGTFVATESNVSRPT"
     gene            complement(11079..>11292)
                     /locus_tag="VD791_03580"
     CDS             complement(11079..>11292)
                     /locus_tag="VD791_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07083.1"
                     /translation="TEDPTMTTIIKLLTGLLIGALLSTSAVAGNRIDVLMVQGAQQAK
                     FNLGDSRCVLVGDVIKCIPVITTAAN"
BASE COUNT         1776 a         3927 c         3837 g         1752 t
ORIGIN      
        1 agtgctgggg cggctcaaga tctgcgggcc ggacgcggag gagctcccgc cgggcgaggt
       61 cggtggggtc tatttcgccg acggtcggcc gttcgcgtac cacaacgatc cgcagatgac
      121 cgcggaagcg cacaacgcca aaggctgggc cacgctgggc gacatcggct acgtggacga
      181 ggaaggttac ctgtatctga ccgaccgcaa ggcctatgtg atcatctcgg gcggcgtcaa
      241 catctacccg caggagtgcg agaacctgct ggctatgcac cctggcgtgc acgacgtggc
      301 tgtgttcggc gtgccgaacg acgagttcgg cgaagaagtg aaggccgtgg tgcaaccgca
      361 cgacatgcgc gacgccggcc cggcgctcga ggccgagctg atcgctttct gccgccagca
      421 tctttcaccg atcaagtgcc cgcgctcgat cgacttcgag gcgcagttgc cgcgcgagcc
      481 gaccggcaag ctgcagaagc ggcggctgcg tgagcgctat tgggcggggc tgggcggcaa
      541 cacgccacgc cgcacctgat cttcctcgat cgactcgaac agcgcgcgga agttgccctc
      601 gccgaaacct tcgttgccct tgcgctggat gatctcgaag aagatcggac cgatcaccgt
      661 cttggtgaag atctgcagca gcagcccgcc gccctgggtc ggcgcgccgt cgatcaggat
      721 gcggacggcg cgcaggcgcg cgaggtcctc gccgtggccg ggcacgcgct cgtccacccg
      781 gtcgtaatag acgtcgggcg tgtcgaggaa ggcaacgccg gccgtccgca gcccttccac
      841 ggtggcgtag atgtcgcggc tggcgagcgc gatgtgctgg atgccctcgc cgtgataggc
      901 gtcgaggtat tcctggatct ggctgcggtc gtcggacgat tcgttgatgg ggatgcggat
      961 cttgccgcac gggctggtca tggccttgga gcgcagcccg gtgagcttgc cctcgatgtc
     1021 gaagtagcgc atctcgcgga agttgaacag gcgctcgtag aactcggccc actcgcgcat
     1081 gcggccgcga tgcacgttgt gcgtcaggtg atcgacggtg ctcagccccg cgccggccgg
     1141 ccggcggtcc tcacccggca gccagcggaa atcgacatcg tagatcgaca ggtcgccgtc
     1201 gttgccggga tagcggtcca ccagatagac gagcgagtcg ccgatgccct tgatcgccgg
     1261 aatgttgagc tccatcggcc cgggttggct gggcacgccc caggcgccca ggtcgatggc
     1321 acgcttgtag gcggcccccg cgtcgcgcac ccggaacgcg atcgcgcaca cgcacgggcc
     1381 gtggatgcgc gcgaaggact gcgcgaagga gtgcggctcg gcgttgatga tgaaattgac
     1441 gtcgccttgc cggtacaggg tgacgtcctt ctgccggtgc cgcgccaccg cgcggaagcc
     1501 catctttacg aacagcgccc cgagcgctgc cggatcgggc gccgcgtatt cgacgaactc
     1561 gaagccgtcg gtgcgcatcg ggttgtccca cagatcgggc atggatctta cctctcaccc
     1621 ccgcccctct cccgcaaggg gagaggggtg tctacttggt gtcctcgaac agctcgcggc
     1681 caattagcca gcgccggatc tccgatgtgc ctgccccgat ctcgtagagc ttggcgtcgc
     1741 gccacaggcg gccggtcgga aactcgttga tgtagccgtt gccgccgagc gcctggatcg
     1801 cttcgcccgc catccaggtg gccttctccg cgctgtacag gacggcgccg gcggcgtcct
     1861 tgcgcagcgc gcgcacctgc tcgggcgagt tggccgcatc gcacgcgcga ccgaccgcgt
     1921 agacataggc gcggcaggcg ttcatcgtgc tgtacatgtc ggcgagcttg ccctgcatca
     1981 gctggaactc gccgatcggc tggccgaact gcacgcgctg gtgcacgtag gggagcacga
     2041 cgtccatgca ggcctgcatc acgccgagcg gcccgccgga gagcacggca cgctcgaagt
     2101 cgaggccgct catcaggatc tgtgcgccgc ggcccacctg ccccagcacg ttctcctccg
     2161 gcacttcgca gtcctggaac accagctcgc cggtgttgga cccgcgcatg ccgagtttgt
     2221 cgagcttctg cgccgtggag aagcccttga accccttctc gatcaggaaa gcggtgatgc
     2281 ccttcgcacc ggccgccgga tcggtcttgg cgtagaccac cagcacgtca gcgtccggcc
     2341 cgttggtgat ccacatcttg ttgccgttca gcaggtaacg gtcgccccgg cgctcggcgc
     2401 gcaggcgcat gccgaccacg tccgagccgg cgttcggctc gctcatggcc agcgcgccga
     2461 cgtgctcgcc gctgatcagc cgcggcaggt acttgcgctt ctgggcggcg tcgccgttgc
     2521 ggcggatctg gttgacgcac aggttggagt gcgcgccgta ggacaagccg acggctgcgg
     2581 aggcgcggct gatctcctcc accgcgagga tgtgggcgag gtagcccatg ccggcgccgc
     2641 catactcctc ctcgaccgtg atgccgagca gccccatgtc cccgaactta cgccacaggt
     2701 gcgcagggaa ctcgttgctg cggtcgatct cggcggcgcg gggcgcgatc tccgcggcgg
     2761 cgaactgctg cacgctctcg cgcagcatgt cgagggtctc gcccaggccg aagtcgaggg
     2821 tggtgaagcg cttgaggatg ggggcgtcgg caggtttcac ggcagtgctc cggaggcatc
     2881 gaccccgcag atgttaacag gggcggacgg ccccatgaag cgctaaaccc gggcgccggc
     2941 aagccgatac cactgtccta tacgccgacc cacctcatca aggaggtcct catgcaaagc
     3001 aatccctaca agacgcccgc cgccaaggtc gccgaccagg ccgagcagta cggcgaggtc
     3061 aagatcctgt ccgcttccgg ccgcatgggc agagtccgtt acatcggcta cggcgtggga
     3121 atgacgctgg tcatgtacct ggtgatcggg ctgttcgccc tgctcgccgg ggtgaccgat
     3181 cttcagttcc tgctcgtggc cggcatgatc gttggcggcg tcggcatgct ggtggtcatg
     3241 gtcatgctca cgatccagcg ctgccacgac ttcaacatgt cgggctggat gtccctgctg
     3301 ctgatcgttc cgatcgtccc gctgctgttc tggatcatcc cgggcacgca gggctccaac
     3361 cgcttcggca accccccgcc gccgaactcc accggcgcgg tggtcctggc gctgatcctg
     3421 ccggtgctgt tcgtggtcgg gatcatggcg gccatcgccg tccccgccta cgtggattat
     3481 cagcagcgcg cggcgcaggc ggagcagtcg cagccgacgc agtagcctgg gagagattgc
     3541 accccgcctc tgacgaggcg gggtgcccgc gccgcgggcg gggcggtgtg gtgttgaacc
     3601 gctggaccac cccggcgctc cgcgccaccc ctccttgaga aggaggggaa cgctcacatc
     3661 ccggggcccg cgcagcgggc cggatcggtt atcattttgt tctgcaaggg cagcggcccg
     3721 ctctggccac tgccctttct acgatgaacg atcccgccgc tttccggccc gccccggccg
     3781 gcctcaaccc tctgcagttc ccgttcgccg gtccgccgga ggcgggaacc acgctcgaga
     3841 tcgcgccggg catgttctgg ctgcgcatgc cgctgccgtt cgcgctggac cacatcaact
     3901 tgtgggtgct gcgcgacggg gacggttgga cgttgatcga ttgcggcctc aactccgccc
     3961 gcacgcgcga gctgtgggag gcgatctgcg gttcgctgct ggccacgggc agcgtcaacc
     4021 gggtgctggt gacccacttc catccggacc acgtcggcct cgcgggctgg ctgacgcagc
     4081 gcttcggcgt cgagctgtgg atgaccgaag ccgagttcct caccacccat gtcaattacg
     4141 cgcagctgcc cgggcacacc aaggacggca ccctggcgct gtttcagcgg cacggtctgg
     4201 acagcgcacg cgcggaggtg atgcgcgcgc acccgcacgt gtaccggcgc gccatcagcg
     4261 agccccccgc cgcgttccgc cgcatcatgg acggcgaccg catcgacatc ggcgagcacg
     4321 cctggtcggt ggtgaccggc tatggccatg cgcccgagca cgcggccctg tactgccgcg
     4381 agctgagcct gctgatttcc ggcgacatgg tgctgcccaa gatcagcacc aacgtcagcg
     4441 tttgggccga gcagcccgaa gccgacccgc tggacctgtt cctcaagtcc gtggatcgct
     4501 acgcgcagct gccgggcgac acgctggtgc tgccttcgca cggcctgccg ttccgcggct
     4561 tgcaggcgcg cgccgcggcg ctgcacgagc accacgccgc gcgcctcgac gaggtgctgg
     4621 cggcctgcgc gcagccggtt tccgccgcgc agatggtgcc ggtgctgttc cggcgcgcgc
     4681 tcgacgacca ccagatgtcg ttcgccatgg gcgaggcgat cgcgcacttg aatcacctgc
     4741 tgtacaaggg ccgggtggtc cgcagcgagc aggacggcat cctgtgcttc gccgcggtgt
     4801 gagcagcaag cctcgatgac cgctcccagc gattccgtgc cgccgtcctc cccgtcgccg
     4861 ccgcggccgg aggagctggc gcgcgagttc gcggacatcg cgcgccagag ccagcgtctg
     4921 gccaacgact tcctggcgca cgtgcagcag ggcaaggcgc cgctgcccag cgacgacgtc
     4981 ggcgtgacca atgcgttcgt cgatctggca gccaagttga tggccaaccc gatgcagctc
     5041 gccgaggcgc agatgcgcat gtggcacgac tacgcgcgcc tgtggcaaag cgccatggcg
     5101 cgcttcctcg gcgagacgcc ggagccggtg gccaccccgg ccaagtccga caaccgcttc
     5161 aagaacgagg tgtgggagaa caacttcctg ttcgactaca tcaagcagtc ctacctgatc
     5221 gccgcgcaga acatccagcg cacggtgagc caagtgcagg gcctggatcc gcagaccgcg
     5281 cgcaaggcgc agttcttcac gcgccagttc gtggacgcgc tggcgccgac caatttcgtg
     5341 ttcaccaacc ccgaggtgct gcacgcgacg gtggagtcgg gcgggcgcaa cctgatcgat
     5401 gggttgcaca acctgcttga cgacctggag cgcggcggcg gccagctttc cgtcagcatg
     5461 accgacccct cggcgttcaa gctgggcgag aacgtcgcca ccacgccggg caaggtggtc
     5521 tatcagaacg acctgatcca gctcatccag tacacgccga gcacgcagac ggtgtggaag
     5581 acgccgctgc tgatcatccc gccctggatc aacaagtatt acatcctcga cctgcgcgag
     5641 aagaattcct tcatccgctg ggcggtggcg cagggccaca cgttgttcgt gatctcctgg
     5701 cgcaacccgg acgcgcgctt cgccggcaag ggcttcgacg actatatgct ggagggtcca
     5761 atcgcggcgc tggaagccat cgagcgggcc accggcgaga aggaggcgaa cgtcatcggc
     5821 tactgcctgg gcgggacgct gctcggctgc acgctggcct ggctggcggc gaagcagcgc
     5881 aacccggtga agagcgcgac cttcttcacc gcgctgatcg acttcaccga gccgggcgag
     5941 ctgggcgtgt tcgtcgacga gaccgtgctg gcgaacctgg aaaagaagat gcaggagcgc
     6001 ggctatctcg agggctcaga gatggcggcc accttcaacc tgctgcgcgc gaacgatctg
     6061 atctggtcgt tcgtggtgaa caactacctg ctcggcaagg acccgttccc gttcgacctg
     6121 ctgttctgga attccgactc cacgcgcatg ccggcgaaga tgcacacctt ctacctgcgc
     6181 aacatgtacc tccgcaacgc gctggtgcag ccgggcggga tcacgctggc gggcgagccg
     6241 atcgacctcg gcaagatccg cacgccggcc tgcttcgtgt cgaccatcga ggaccacatc
     6301 gcaccctgga agtccaccta cctgggcgcg cggcgcctgc cgggcgaggt caagttcatc
     6361 ctcggcggct cgggacacat cgccggcatc gtcaacccgc cggcgggcgg caagtatcac
     6421 cactggaccg atgacacgct caccggcagc gccgacgagt ggctgtccca ggcgcagcgg
     6481 cgcgacggct cgtggtggcc ggagtggaac gcctgggtcg aacggttcgg cggcgagaaa
     6541 gtcccggcgc gtgcaccggg cgacggcagg ctcgagccga tcgaggacgc gccggggtcc
     6601 tacgtcaagg agcggctgga cacgcagcgg taggcctaca tcccggcgcg caccgcgcgc
     6661 aggtcggtgc gcgccgccga catcggcagc agcgcgcgca gctcctcggc ccgcatcggt
     6721 ttggccacca gataaccctg gatctcgtcg cagcggtgct cgcgcaggaa ggcgagctgc
     6781 gcctcggtct ccaccccttc ggcgagcacg tcgagcttga ggctgcgcgc catcgcgatg
     6841 atggcgcgca ccagcgcgcg gtcgctggga tcggtctcca ggtcgcgcac gaacgcgcgg
     6901 tcgatcttca ggcggtcgaa gggcagcagc ttgagcgagc tcaagcccga gtaaccggtg
     6961 ccgaagtcgt ccagcgccag gtgcacaccc aagcgcttga gccggccgag catctccatc
     7021 gcctccgcgc cggtctgcaa cgccccctcg gtgacctcca gctcgagcag cgcgggatcc
     7081 aggtcggtct cgcgcaacgc cgacaccacg tgcgtgacga tggcctcgcc cgcgatctgc
     7141 tgcgccgaga tgttcaccgc catgcgcatc ggcggatggc cggcgtcgat ccaggccttg
     7201 gcctgggcgc aggcggtgcg caacacccat tcgccgatgc cgtggatcag gccgatctgc
     7261 tcggccaccg gtatgaagtg atgcggcccg agtagccccc gcgtcggatg ctgccagcgg
     7321 atcagggcct ccacgcctac ggtgcgcgcc tgggcgatgg agacctgggg ctgatagtgc
     7381 agcaccagct gctcgagcag gcgcgggtcg cgcagcgtgt tctccagggt cagccgctcc
     7441 atcgcctgca ccgtcagctc ctcggagaag aactcgtagc gtccctgccc ggtgcgcttg
     7501 gcgcggtaca tcgcagcgtc cgcggccttc aggagctgct cggcggtgtt gccgtgcgcc
     7561 gggcccaggc tgatgccgac gctggcgcgc atgcgcagct cgatgccgcc gagctcgaag
     7621 ggcgcggaca gcaccgaaat gatcttctcg gccacgcgcg ccggcgcgcg cggctcgctg
     7681 acatcctcca cgatcaccac gaactcgtct ccgccgagcc gggcgaccac gtcctgcccg
     7741 cgcacgcatc cctgtagccg gcccgccatc gcctgcagca ggcggtcgcc ttcctggtgg
     7801 ccgagggtgt cgttcacgtg cttgaagctg tccaggtcca ggtacagcag tgccaccgcg
     7861 cgaccggtgc gttcggcgcg gctcagggcc gagcgcagcc gttcgcccag cagcagccgg
     7921 ttgggcagcg tggtgagcgg atcgtgacgg gcgaggtagt tgagccgctc ctccgccgcg
     7981 atgcgctcgc gcaggtcctg ggcgatgcac acgatcgccg gcgcggtacc cggctcgcgc
     8041 atgggcacgg cggacagcgt caccggcacc cgcgatccgt cgcgccccag gaggcgcaca
     8101 tcgaagcggg ccggcaacgg tccctggacg ccttccagca gccgttcgct tcccccgtcg
     8161 acgaccgcgg agaacaccag cccgcacaac tgctcgcgct cgtatcccag cagccgggcc
     8221 gccgccgggt tcgccgtctc gatcgccccg tcgtgcgaca ccaccagcag ggcctcgccc
     8281 atggaatcga gcacgctctc catgtagctg cgcgacacgg tggtggcctg cagccgctcg
     8341 gccatctcgt tgaacgccac cgtcagcttg cccagctcgt cgctgccccc gctcggcaca
     8401 cgcagcgaga aatccccttc cgccacgcgg cggctggacg ccaccagcgc gcgcaacggc
     8461 gccctcaccg cgcgcgtggt ggccgcggcg accagcacgc cgagcgcgag cgccaggagc
     8521 aggccgcgcg tcaccagcag attcgcgtcg tgcgcagtgg ccgaagcgcg ggccacggct
     8581 tcgttcagct cggtgcgcgc ggcgggatgg atgcgctcgt cgagcagctg ctcgagcgct
     8641 tcgccctgcg cgccgaggtc ggcaagtgca cgatactgcg tgcgttcgcc cgcgaggatc
     8701 gcgcgcccct ggcgatcgct ctgctccagc cagcggtgta cgctcgctgc ccattggcgc
     8761 tccgcgccgg tcctggcgaa cgcttcgtag ctcgccaacg ccgcgtccac gcgccgccgc
     8821 gcgccgtcga tccggggcca tgcatccgcg acctcgggtt cggaggtgag cacgcctgca
     8881 acctggaagt agcggccgag cgccgtctcc accgcactcc cgtgcggccg gggcgattcg
     8941 cgcgcagccc agacgcgcgg cagcgcgcgc agcagcgcgt cgccctcgcg ctggtgggcg
     9001 tcgtaggccg cgagacggcg tgcatgactg cccgcctgct ggatcaacgc cgccgcacgc
     9061 tgcttgaagc gcgcgtagcg ccgctcgacg tcgcgatgca ggccgcggct gccgtcggtg
     9121 gaggcgaccg cagcgtagct cgacaacgcc cgctcgaatc ccgcttgcgc ctcgctgagc
     9181 tgtttgcgct gagccggttc gtgcacgtcg gagacgcttt gcaccgctcg cgtcatgcgg
     9241 tcgagcgcga gctgcatctc gtatgccgcg gcgctgcgcg cttcgctgtc ctcgaccgct
     9301 tccgacaaca cggcgtcgag cgagtgcgtg gcgcgcaccg atacgtagtg cgcggccgcg
     9361 aacagcgcca ggatcaggcc gagcccgagc cagagtcgtt gcgagattgt caaccgcagg
     9421 ccgatcaccg tggagaaagt ccggacgcag tgaggattca gcccgggcct gcactcaaga
     9481 tccgcaccag gagccgaggg caagcggggt acgtacgaga gagggcgcgc cgtagtcgcc
     9541 ggcagccgac gtcttgcgcg cacctactcc cggacgtcgt cgtgcgcgag cgccccgtcg
     9601 tcgcggacgc gttcgtgtgg ccccgctgcg acctcctggc ccagccgcgg cgcgctcccg
     9661 aagcggccca ccatggcgtc gaagatccag cgcagctcgc cgacctcatc gccgcgcatg
     9721 gacagactgc gatacgacag atcgccgtcc acgtagccgc gcaccgaggc gatcagccgg
     9781 cgcagcggcg cccgcacggc gaagctggtg acggccgcga tcgccgcacc cagcatcagg
     9841 gccgccagca agccgttcga caacagctcg agagtccgca ccgcgcttgc cagcgcgggg
     9901 gcatgcgcgt cgccttgcgt ttcgagcgtg ctcagcatgt gcagggcgaa caggtaggct
     9961 gccgcgagcg gcgacagcag gagcccaagc ccgatccaga ggcgctgggc gatggtcagc
    10021 cggatcttca tcggggcgct ccgccgttca ttcttcctcc tggtcatggg tggtgaaggg
    10081 aagagagggg cgcgggggga gcgtgatact gtagtgcgcc cgcgcgcccg cggctactgc
    10141 cgccgatgag cgacaggccc cagcgcgccg cacctcgaac gagcgaccga gaccatgaag
    10201 accatcctga tcaccggcgc ggccggagac atcggcaccc gcctgcggcg cgagctggcc
    10261 ggcgtgtacc aattgcagct gtccgacatc cgtccggtgc ccgcgctcgc cccgggcgaa
    10321 gtcttcgagc gcggcgacat cgccgacctg agcgacatgc tgcgcatcgc ccgcggcgtg
    10381 gacgccatct tgcacctggg cggggtgtcg gccgagtccg actgggagtc gatcctgcag
    10441 gccaacatcg tcggctgcta caacgtgttc gaagcggcgc ggcgcaatgg cgtgaagcgc
    10501 gtgctgttcg ccaccagcaa ccacgcggtc gggttctacc ggcgcgacga gaccatcgac
    10561 caccgcgtct atccgcgccc cgacagccgc tacggcctgt cgaaggtgtt cggagaacag
    10621 ctcggcagcc tgtacgccga caagtacggc ctggaggtgt tctgcatgcg catcggcaac
    10681 ctcacccgcg cgccggtgga caagcggcga ctgtcgatct ggatctcgcc gcgcgacctc
    10741 gcgcagctgg tgcgcatcgg cgtcgagcat ccggcgatcc gcttcgagat cgtgtacggg
    10801 gtgtccgcca accgccgctc ctggtacgac aactccaacg ccgagcggct gggctaccgc
    10861 ccgcaggacg acagcgagcc gtacgcggcg gagatcctcg ccaagcacgt ggacgcggcc
    10921 gacccgcggg tggagctgta tcagggggga actttcgtgg cgaccgaatc gaacgtgagc
    10981 agacccacct gaccgcgcca gaccccggca caaataaagc ggcccgcgcg aggcgggccg
    11041 aaagggggag gagacgacga agaaacggtt cgggcagatc agttcgcggc ggtggtgatc
    11101 acggggatgc acttgatcac gtcgccgacc aggacacacc tggaatcgcc caggttgaac
    11161 ttggcttgtt gcgcgccctg gaccatgagc acatcgatac ggttgccggc cacggcgctg
    11221 gtcgaaagca gcgcgccgat cagcaggccg gtcagcagtt tgatgatggt cgtcatggtc
    11281 gggtcctcgg tt
//
LOCUS       DASPGE010000112         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_969619, whole genome shotgun sequence.
ACCESSION   DASPGE010000112 DASPGE010000000
VERSION     DASPGE010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969619"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..>2196)
                     /locus_tag="VD791_03585"
     CDS             complement(282..>2196)
                     /locus_tag="VD791_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXV07084.1"
                     /translation="ADWSKVRAEYADVNEHVRRNRIWGAMSTGGSRGIRDSQSYVRAG
                     GAAAREMLVAAAAQQWGVPAAECGVAKGVITHAGSGRSTTFGKVAAAASKLEAPKEPK
                     LKDPKDWKLIGTSPARFDIPDKTTGKQIYAADVRPAGLLHASVAQSPVFGGKLKRYDE
                     AKIKGMRGVKRVVAGPDWVAVVADNWWRANQALKAMPIEWDPGENGQVSSQSIMAFLR
                     TGIDAKEAPVARKDGDFDKAAAGAAKVLEAEYYNPYMNHATMEPQTATALVTDDKVEV
                     WVGTQNGEASMAAASEASGVPLEQVYVHKMHAGGGFGRRGPHQEYTKQAVVIAQAMRG
                     TPVRLQWSREEDIQQGRYRPVGLIRLRGALDAQGNWIGWHVRQADQSIISTVRLATLQ
                     VKDGIDPVNTRCFSDNPYAVPNFTNEYSMRNTHVPPGFWRAVAHTNNPIAREGFIDEL
                     AHAAGKDPYEFRRPLLQGKKDLGVLDAVAKAAGWGKPPAKGVHRGIAVVDSYGSYTAA
                     VVELSMKGEAIDVQRVLVAIDCGHVCHRDAVIGQIEGGVIWGLSAAMHEEITIEDGRV
                     AQSNFSDYPLLRLGETPPKIEAILVPTGGFWGGVGEPPIGGVLPALCNAIFAATGKRI
                     RSLPLKHHGFSYA"
BASE COUNT          292 a          825 c          726 g          353 t
ORIGIN      
        1 cttcggcagc tccagcacca ctttgccggg cgtgatcggg cgctcgccga aggtttcgcg
       61 cagcgccgcc gcgacctcct ccgcggccgc gcgggccggg cgcacggcga gcaggcacac
      121 gccggcggcc agcgcacgca aagccgcgcg gcgggacgtc acaccacgtg cgcccttaag
      181 gcgcgacatg agaaaaccct cccctcagcg ggaggcagcg ggccacaggc ttccagcgct
      241 gcgtgacgac actgcggcac cgcccaccaa ccctcccccg gtcacgcgta cgagaagccg
      301 tgatgcttca gcggcagcga gcggatgcgc ttgccggtcg cggcgaagat ggcgttgcac
      361 agcgccggca gcacgccgcc gatcggcggc tcgccgacgc cgccccagaa gcccccggtg
      421 ggcaccagga tcgcctcgat cttcggcggc gtctcgccca agcgcagcag cgggtagtcg
      481 gagaagttgc tctgcgccac gcgcccgtcc tcgatggtga tctcctcgtg catggcggcg
      541 ctcaagcccc agatcacgcc gccctcgatc tgtccgatca ccgcgtcgcg atggcacacg
      601 tggccgcagt cgatcgcgac cagcacgcgc tgcacgtcga tcgcctcgcc cttcatggag
      661 agctcgacca ccgcagcggt gtagctgccg taggaatcca ccaccgcgat gccgcggtgc
      721 acgcctttgg ccggaggctt gccccagccg gccgccttgg ccaccgcgtc gagcacgccg
      781 aggtccttct tgccttgcag gagcgggcgg cggaactcgt aggggtcctt gcccgcggca
      841 tgcgccaact cgtcgatgaa gccctcgcgc gcgatcgggt tgttggtgtg cgccacggcg
      901 cgccagaagc cgggcggcac gtgcgtgttg cgcatcgagt actcgttggt gaagttcggc
      961 accgcgtagg ggttgtccga gaagcagcgc gtgttgaccg gatcgatgcc gtccttgacc
     1021 tgcagggtcg ccagccgaac cgtgctgatg atcgactggt cggcctgccg cacgtgccag
     1081 ccgatccagt tgccctgggc atcgagcgcg ccgcgcagcc ggatcagacc caccggccgg
     1141 tagcggccct gctggatgtc ctcctcgcgc gaccactgca gccgcaccgg ggtgccgcgc
     1201 atcgcctggg cgatcacgac cgcctgcttg gtgtactcct ggtgcggccc gcgccgcccg
     1261 aagccgccgc ccgcgtgcat cttgtgcacg tagacctgct ccagcggcac acccgaggcc
     1321 tccgaggccg ctgccatgct cgcctcgccg ttctgggtgc ccacccacac ttccaccttg
     1381 tcgtcggtga ccaatgcggt cgccgtctgc ggctccatgg tcgcgtggtt catgtagggg
     1441 ttgtaatact cggcctcgag caccttggcc gcgcccgcgg ccgccttgtc gaaatcgccg
     1501 tccttgcgtg cgaccggcgc ctccttcgcg tcgatgccgg tgcgcaggaa ggccatgatc
     1561 gattggctcg acacctggcc gttctcgccg gggtcccact cgatcggcat ggccttgagc
     1621 gcctggttcg cgcgccacca gttgtcggcg acgaccgcca cccagtccgg ccccgcgacc
     1681 acgcgcttga ccccgcgcat gcccttgatc ttggcctcgt cgtagcgctt gagcttgccg
     1741 ccgaacaccg gcgattgcgc gaccgacgcg tgcagcagcc cggccgggcg cacgtccgcg
     1801 gcatagatct gcttgccggt ggtcttgtcg gggatgtcga agcgcgccgg cgaggtgccg
     1861 atcagcttcc aatccttcgg gtccttgagc ttcggctcct tgggcgcctc cagcttggag
     1921 gcggccgccg ccactttgcc gaaggtcgtg ctgcgcccgc tccccgcgtg cgtgatcacg
     1981 cccttggcca cgccgcactc cgcagccggc acaccccatt gctgggcggc cgccgccacc
     2041 agcatctcgc gggcggcggc gcccccggcg cgcacgtagg actgggaatc gcggatgccg
     2101 cggctgccgc cggtggacat cgcgccccag atgcggttgc ggcgcacgtg ctcgttcacg
     2161 tccgcatatt cggcgcgcac cttgctccag tcggcc
//
LOCUS       DASPGE010000113         8579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1672893, whole genome shotgun sequence.
ACCESSION   DASPGE010000113 DASPGE010000000
VERSION     DASPGE010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8579
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672893"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /gene="thiC"
                     /locus_tag="VD791_03590"
     CDS             <1..1081
                     /gene="thiC"
                     /locus_tag="VD791_03590"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HXV07085.1"
                     /translation="IHETREWIVRNSPVPIGTVPIYQALEKVNGKAEDLSWEIFRDTL
                     IEQAEQGVDYFTIHAGVRLPFIPLTAKRMTGIVSRGGSIMAKWCLAHHRESFLYAHFE
                     DICEIMRAYDVSFSLGDGLRPGSIYDANDEAQIAELKTLGELTDVAWKHDCQVMIEGP
                     GHVPMHLIKENMDLQLQHCKEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAML
                     CYVTPKEHLGLPDRDDVKDGIIAYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWQD
                     QFNLGLDPDKARQFHDATLPQEGAKLAHFCSMCGPHFCSMKITQDVRDFAAKHGLSEQ
                     EALGKGMEQKAEEFVRRGGEIYSKA"
     gene            1113..1703
                     /locus_tag="VD791_03595"
     CDS             1113..1703
                     /locus_tag="VD791_03595"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HXV07086.1"
                     /translation="MGVPQKAVAWIKPEEYLALEEKATTKHEYLDGVVYSWQGFVPEA
                     MAGGSRAHNRVIQNLVFALRSSTPRRPCEVYASDIRLNVSAGRAYFYPDVAVTCSPGD
                     SAAAAAQASEFSEPCVVFEVLSDSTEAFDRGEKFEAYKYIPTLEEYVLVSPLNRRVEV
                     FRRAQGWSAQICDSGAVRLDCLDISVALDRVFEGLD"
     gene            1776..2021
                     /locus_tag="VD791_03600"
     CDS             1776..2021
                     /locus_tag="VD791_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07087.1"
                     /translation="MLANEAAERLLARDAQGAASVLAALKREDPAYPAAGALSVLALD
                     REREGSSRALIGLRQRLRDLNRDLFALYMQRRTVFHR"
     gene            2032..2862
                     /locus_tag="VD791_03605"
     CDS             2032..2862
                     /locus_tag="VD791_03605"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HXV07088.1"
                     /translation="MDFSLAVKALILGIVEGLTEFLPISSTGHLILAGQLLDFTGERA
                     QVFAIAIQTGAILAVCWEYRARLLGAVAAFGRERAARRFFANLAIAFVPLAVLGLLYG
                     TRIKATLFHPIPVAIAFIVGGLVILWAERRKHRITVEKVDEMRWPDALKLGVAQAFAL
                     IPGTSRAGATIIGGLLFGLSRKAATEFSFFLAIPTLITAGAYEMIKHRALFSADDLNL
                     LAVGLIASFASALLAVRALLRYISTHDFTAFAWYRIGFGLLVLATGYAGVIDWGAAPY
                     "
     gene            complement(2859..3311)
                     /locus_tag="VD791_03610"
     CDS             complement(2859..3311)
                     /locus_tag="VD791_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXV07089.1"
                     /translation="MANISEILSNARTRAIERKLSYQGALFPAEAYALQQRAPGAKIV
                     DVRTRAELDYVGRIPGSVEIEWQTYPGGQPNPAFLAELQQQVDKESLVMFVCRSGARS
                     HAAATAAAGVGYTQCYNVLEGFQGDKDGQGHRDAVGGWRVAGLPWYQS"
     gene            3589..5001
                     /gene="glnA"
                     /locus_tag="VD791_03615"
     CDS             3589..5001
                     /gene="glnA"
                     /locus_tag="VD791_03615"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HXV07090.1"
                     /translation="MTTAADALKLIKENEVKFVDLRFTDTRGKEQHVSVPVKAFNMSK
                     FEDGHAFDGSSIAGWKGIQASDMLLLPDPATAHMDPFTDEPTLILTCDVIEPSDGKGY
                     DRDPRSIAKRAEAYLKSTGLGDTAYFGPEPEFFIFDSVTWNVDMSGCSVRIVSEEAPW
                     TSGEDLEGGNKGHRAPIKGGYFPVPPVDSLQDIRSAMCLALEEQGVEVEVHHHEVAAA
                     GQNEIGTKFAPLVQRADWMQILKYTVHNVAHSYGKTATFMPKPIVGDNGSGMHCHQSL
                     WKEGKNLFAGSGYSGMSDLALHYIGGIIKHARALNAITNPGTNSYKRLVPGFEAPINL
                     AYSARNRSAAVRIPYVTSDKARRIEVRFPDPMMNPYLGFAAMMMAGLDGVQNKIHPGD
                     PVDKNLYDLPPEEAKKVPNVCSSLEMALEYLEKDREFLTRGGVFSNDMLDAYMELKTE
                     ELNNFRMTTHPIEFAMYYSL"
     gene            5192..5665
                     /locus_tag="VD791_03620"
     CDS             5192..5665
                     /locus_tag="VD791_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HXV07091.1"
                     /translation="MRLPAALLALLVAPLLASLAPSVAWAQTCKYVDNEGRVTYSNVP
                     VKNARKVTCFEPVKPPPGAQSAPPAAAAQRPTVDTNTQRQRDDERRGILESELAAEQQ
                     RLDEARRALAEQEAIRSGDERNYQRVLERLKPYQEAVAQHEKNIASLRQELANLR"
     gene            5709..6785
                     /gene="glnL"
                     /locus_tag="VD791_03625"
     CDS             5709..6785
                     /gene="glnL"
                     /locus_tag="VD791_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HXV07092.1"
                     /translation="MRAFASPGLDALATAVLLLDRGGAIDYANAAAENLLKLSTRNIL
                     GHRLDEVFLDATPLLAAAQYAEAHHCGYTQHDLILATTAAEKLELTCTVTPAEIEAFD
                     GFLLEFTETHQQLRIAREERLQDQTEASRALIRNLAHEIKNPLGGLRGAAQLLERELD
                     RPELAEYTQVIMQEADRLQSLMDRLLAPNRIPQPAPLNIFECLERVRSLLLAEYPELT
                     IRRDYDVSLPPVTADKEQIIQALLNIGRNAAQALKGRGEIELRTRVARQVTLARKLHR
                     LGVMLQIVDNGPGIPAELREKIFFPLVSGREGGTGLGLTIAQNFIHQHGGVIEVESEP
                     GRTCFTIVLPLEARREAGEPPEAR"
     gene            6804..8207
                     /gene="ntrC"
                     /locus_tag="VD791_03630"
     CDS             6804..8207
                     /gene="ntrC"
                     /locus_tag="VD791_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HXV07093.1"
                     /translation="MKPVWIIDDDRSIRWVFEKTLAREDIPFKTFSSAQDALGALPAG
                     APQVVVSDIRMPGGSGLELLQKLKERHPHLPVIIMTAYSDLESAVAAFQGGAFEYLPK
                     PFDVDHAVDLIRRAMEESLRQGEAGEVSEAMPEILGQAASMQEVFRAIGRLTQSHATV
                     LINGESGTGKELVARALHRHSPRAAKPFIAINTAAIPKELLESELFGHERGAFTGAQS
                     MRRGRFEQAEGGTLFLDEIGDMPADLQTRLLRVLSDSHFYRVGGHQPIKANVRIITAT
                     HQDLESRVKQGLFREDLFHRLNVIRVRLPALRERREDIPLLARYFLGKSARELGVEPK
                     RLTDAAMKYLMSHDFPGNVRQLENLCHWLTVMAPGVSVDLHDLPPEFRQDQRAMTTQS
                     WIAALEREAELALNRGEHSIMPELSRQFERALIVKALAHTGGRRIEASHLLGMGRNTL
                     TRKIQELEIESKDAAGE"
     gene            complement(8217..>8579)
                     /locus_tag="VD791_03635"
     CDS             complement(8217..>8579)
                     /locus_tag="VD791_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HXV07094.1"
                     /translation="HPRLALLGDFNVAPEERDVHDPRLWEGKVLFSAPERDALRRLFD
                     LGLADSFRLFEQPERSFSWWDYRMNQFRRNMGLRIDHILLSRPLAKQAKASAIHKQVR
                     AQERPSDHAPVVTELGES"
BASE COUNT         1489 a         2963 c         2820 g         1307 t
ORIGIN      
        1 catacacgaa acgcgcgagt ggatcgtgcg caacagtccg gtgccgatcg gcaccgtgcc
       61 gatctaccag gcgctcgaga aagtgaacgg caaggccgag gacctgagct gggagatttt
      121 ccgcgacacg ctgatcgagc aggccgagca gggcgtggac tacttcacca tccacgccgg
      181 cgtgcgcctg cccttcatcc cgctcaccgc caagcgcatg accggcatcg tgtcgcgcgg
      241 cggctcgatc atggccaagt ggtgcctggc gcaccatcgc gagagcttcc tgtacgcgca
      301 cttcgaggac atctgcgaga tcatgcgcgc gtacgacgtg agcttctcgc tcggcgacgg
      361 gctgcggccg ggctcgatct acgacgccaa cgacgaagcg cagatcgccg agctgaagac
      421 cctgggcgag ctgaccgacg tcgcctggaa acacgactgc caggtgatga tcgaggggcc
      481 gggccacgtg cccatgcatc tgatcaagga gaacatggac ctgcagctcc agcactgcaa
      541 ggaggcgccg ttctatacgc tcgggccgct caccaccgat atcgcgcccg gctacgatca
      601 catcaccagc gcgatcggcg cggcgatgat cggctggtac ggcaccgcca tgctgtgcta
      661 cgtcacgccc aaggagcacc tcgggctgcc ggaccgggac gacgtcaagg acggcatcat
      721 cgcctacaag atcgcggcgc acgccgccga tctcgcgaaa ggccatcccg gcgcgcagat
      781 ccgagacaac gcgctgtcca aggcgcgctt cgagttccgc tggcaggacc agttcaacct
      841 cggcctcgac ccggacaagg cgcgccagtt ccacgacgcg accctgccgc aggagggcgc
      901 caagctggcg cacttctgct cgatgtgcgg gccgcacttc tgctcgatga agatcaccca
      961 ggacgtgcgc gacttcgccg ccaagcatgg cctgtcggag caggaagcgc tgggcaaggg
     1021 catggagcag aaggccgagg agttcgtgcg ccggggcggc gagatctact ccaaggcgta
     1081 gctggcgcgg cggcaatagg agcgtagcga cgatgggtgt gccccagaag gcggtggcct
     1141 ggatcaaacc cgaggaatat ctggcgctcg aagagaaggc gaccaccaag cacgaatatc
     1201 tcgacggcgt cgtctactcg tggcaagggt tcgtaccgga ggcgatggcg ggaggaagcc
     1261 gcgcgcacaa ccgggtgatc caaaacctcg tattcgccct gcgatcctcg actccgcgcc
     1321 gcccctgcga ggtctacgcg agcgatatca ggctgaacgt gtcggccggg cgcgcctact
     1381 tctatcccga tgtcgccgtc acctgttctc cgggagacag cgcggcggca gcagcccaag
     1441 catccgaatt ctcggagccg tgcgtggtct tcgaggtctt gtcggactcg actgaggcct
     1501 tcgaccgcgg ggaaaagttc gaggcgtaca agtacatccc caccctcgag gagtacgttc
     1561 tggtatcacc gctcaaccgg cgagtagagg tgttccggcg cgcgcaggga tggagcgcgc
     1621 agatctgcga ctccggggcc gttcggctgg actgtctgga catctctgtc gcgctcgacc
     1681 gggtcttcga aggtctggat tgaggccggg actcgacccc ccaaagcggt tcgagcgtgg
     1741 cgcagcttga tctgttcctg gactcgcgcg cggtcatgct cgcgaacgag gccgccgagc
     1801 gcttgctggc gcgcgacgcg cagggcgccg cttcggtcct cgccgcactg aaacgcgaag
     1861 accccgccta ccctgctgca ggcgcgctga gcgtcctggc tctcgaccgg gaacgggaag
     1921 gtagcagcag ggcgctgatc ggtctacgcc agcggctgcg cgacctcaat cgcgacctgt
     1981 tcgcgctgta catgcagcgt cggacggtct ttcaccgcta gaatcgccag gatggatttt
     2041 tccctcgccg tcaaggcgct catcctcggc atcgtcgaag gcctgaccga gttcctcccc
     2101 atctccagca ccggccacct gatcctcgcc gggcagctgc tcgacttcac cggagagcgt
     2161 gcgcaggtgt tcgcgatcgc gatccagacc ggcgccatcc tggcggtgtg ctgggaatac
     2221 cgcgcgcgcc tgctgggggc ggtggctgcg ttcgggcgcg agcgcgcagc ccggcgcttc
     2281 ttcgccaacc tggcgatcgc gttcgtgccg ctggcggtgc tgggtctgct gtacgggacg
     2341 cggatcaaag cgacgctgtt ccatccgata ccggttgcga tcgccttcat cgtcggcggc
     2401 ctggtgatcc tgtgggcgga acggcgcaag caccgcatca cggtcgagaa ggtggacgag
     2461 atgcgctggc cggatgcgct caagctcggc gtggcgcagg ccttcgcgct gatccccggc
     2521 acctcgcgcg ccggggccac catcatcggc ggcctgctgt tcggcctgtc gcgcaaggct
     2581 gccacggagt tctccttctt tctggcgatc ccgacgctga tcaccgcggg agcctacgag
     2641 atgatcaagc accgcgcgct gttcagcgcg gacgacctga acctgctggc ggtggggctg
     2701 atcgcttcgt tcgccagcgc gctgctggcc gtgcgggccc tgctgcgcta catcagcacc
     2761 cacgatttca cggcgttcgc ctggtatcgg atcgggttcg gcctgctggt gctggccacc
     2821 ggctacgccg gtgtgatcga ctggggagcc gcaccctact aggactgata ccagggcagg
     2881 ccggcaacgc gccagccccc caccgcgtca cggtggccct ggccgtcctt gtcgccctgg
     2941 aaaccttcga gcacgttgta gcactgcgtg tagccgacgc ccgccgcggc tgtggcggcc
     3001 gcgtgggagc gcgcgccgct gcggcagacg aacatcacca gcgactcctt gtccacctgc
     3061 tgctgcagct cggccaggaa agccgggttc ggctgaccgc cggggtaggt ctgccactcg
     3121 atctcgacgc tgcccgggat gcggcccacg tagtccagct cggcgcgggt gcgcacgtcc
     3181 acgatcttgg cgcccggtgc gcgctgctgc agcgcgtagg cttcggcggg aaacagcgca
     3241 ccctggtagc tcagcttgcg ctcgatcgct cgggtgcggg cgttggacag gatttccgag
     3301 atgttcgcca tgtcggcctc cggccgccgc cccaaggcag ggcagcgctt ccactgttgc
     3361 gagtctaggt gcacgatagc ggctcgccaa gtcggtgccg gcgcactaga atagtgcggg
     3421 ttgtgcaggg ctgcaccgat tcggtgccag agatgccggg ttttacgacg taagcgcctg
     3481 tattaaaaca ccgttttctt ccagcgcggg aggcatgcat cgtgcaaaac gccttcggct
     3541 tcagtacgcg ctcgaagaag gttctatttt ttccccagga gacatgaaat gacgacggct
     3601 gccgacgcac tcaaattgat caaggagaac gaggtgaagt tcgtggacct gcggttcacg
     3661 gacacgcgcg gcaaggagca gcatgtgtcg gtaccggtca aggcattcaa catgtccaag
     3721 ttcgaggacg gccacgcgtt cgacggctcc tcgatcgccg gctggaaggg gatccaggcc
     3781 tccgacatgc tgctgctgcc ggacccggcc accgcgcaca tggatccgtt caccgacgag
     3841 cccacgctca tcctgacctg cgacgtgatc gaaccgtccg acggcaaggg ctacgaccgc
     3901 gatccgcgct cgatcgccaa gcgcgccgag gcctatctca agtccaccgg cctgggcgac
     3961 accgcctact tcgggcccga gcctgagttc ttcatcttcg actcggtgac ctggaacgtg
     4021 gacatgtcgg gctgctccgt gcgcatcgtg tcggaggagg cgccctggac ctcgggggag
     4081 gacctggagg gcggcaacaa gggccaccgc gccccgatca agggcggcta cttcccggtg
     4141 ccgccggtcg attcgctgca ggacatccgc tccgcgatgt gcctggcgct ggaagagcag
     4201 ggcgtggagg tggaagtgca ccaccacgag gtggccgccg cgggccagaa cgagatcggc
     4261 accaagttcg ctcccctggt gcagcgcgcc gactggatgc agatcctcaa gtacacggtg
     4321 cacaacgtcg cgcactccta cggcaagacg gcgaccttca tgcccaagcc gatcgtgggc
     4381 gacaacggct cgggcatgca ctgccaccag tcgctgtgga aggaaggcaa gaacctgttc
     4441 gccggcagcg gctactccgg gatgtcggac ctcgcgctgc actacatcgg cgggatcatc
     4501 aagcacgcgc gcgcgctcaa cgcgatcacg aatcccggca ccaactccta caagcggctg
     4561 gtgcccggct tcgaagcgcc gatcaacctg gcctactcgg cgcgcaaccg gtccgcggcg
     4621 gtgcgcattc cgtacgtgac cagcgacaag gcgcgccgca tcgaagtgcg cttccccgac
     4681 ccaatgatga atccgtacct gggcttcgcc gccatgatga tggccggcct ggacggcgtg
     4741 cagaacaaga ttcacccagg cgacccggtc gacaagaacc tgtacgacct gccgcccgag
     4801 gaagccaaga aggtgcccaa cgtctgctcc tcgctggaga tggcgctcga gtacctggag
     4861 aaggaccgcg agttcctcac ccgcggcggg gtgttctcca acgacatgct ggacgcctat
     4921 atggagctga agacggagga gctcaacaac ttccggatga ccacccaccc catcgaattc
     4981 gccatgtact acagcctctg aggccgcgag ctggcgtatc ggtgtgcgaa ggggtgggcg
     5041 cgcgcgcccg cccctttttc gtccatcccg cccgcgcagc tccgcccacg ggcggacgcg
     5101 aattgatccg tgaccgcagc tgtcttagac tggaacgcat gctcacccgg ggtcggcccg
     5161 cggcccgcct cgtccagcac gcccctacac gatgagactg cctgccgccc tgctcgccct
     5221 gcttgtcgcc ccgctcctcg cctcgctcgc cccctcggtc gcttgggccc agacctgcaa
     5281 gtacgtggac aacgagggtc gggtcaccta ctcgaacgtg ccggtcaaga atgcgcgcaa
     5341 ggtgacatgc ttcgagccgg tcaagccccc gccgggcgcg cagtccgcgc cgccggcagc
     5401 cgccgcacag cgcccgacgg tggacaccaa cacgcagcgc cagcgcgacg acgagcggcg
     5461 cggcatcctg gaaagcgagc tggcggcgga gcagcagcgc ctggacgaag ccaggcgcgc
     5521 gctggcagag caggaggcga tccgctccgg cgacgagcgc aattaccagc gcgtgctcga
     5581 gcgcctcaag ccctaccagg aggcggtggc acagcacgag aagaacatcg cttccctcag
     5641 gcaggaactc gccaacctgc gctgagcgcg gcgtgcgcgc cgggcggcgg cggggagagc
     5701 gcctcgccat gagggcgttc gcctcgcccg gactggacgc gctcgccacc gcggtgctgc
     5761 tgctcgatcg cggcggcgcg atcgactacg ccaacgccgc ggcggagaac ctgctcaagc
     5821 tcagcacccg caacatcctc ggccaccgtc tggacgaggt gttcctggac gccacgccgc
     5881 tgctggccgc cgcccaatat gctgaggcgc accactgcgg ctacacccag cacgacctga
     5941 tcctggccac cacggccgcc gagaagctcg agctcacctg cacggtgacg ccggcggaga
     6001 tcgaagcgtt cgacggcttt ctgctcgaat tcaccgagac ccaccagcag ctgcgcatcg
     6061 cgcgcgagga acggctgcaa gaccagaccg aagcgagccg ggcgctgatc cgcaacctgg
     6121 cgcacgagat caagaacccg ctgggagggc tgcgcggcgc cgcgcagctg ctcgagcgcg
     6181 agctggaccg gcccgagctc gccgagtaca cccaggtgat catgcaagag gccgaccgcc
     6241 tgcaatcgct gatggaccgg ctgctggcgc cgaaccgcat cccgcagccg gcgccgctca
     6301 acatcttcga gtgcctggag cgggtgcgca gcctgctgct cgcggagtat cccgagctca
     6361 ccatccggcg cgactacgac gtcagcctgc ccccggtgac tgccgacaag gagcagatca
     6421 tccaggcgct gctcaacatc ggccgcaacg ccgcgcaggc gctgaaaggc cgcggcgaga
     6481 tcgagctgcg cacgcgtgtg gcgcgccagg tcacgctcgc gcgcaagctg catcgcctgg
     6541 gcgtgatgct gcagatcgtc gataacggcc ccggcattcc cgccgagctg cgcgagaaga
     6601 tcttcttccc gctggtgtcc ggccgcgagg gcggcaccgg gctcgggctc accatcgccc
     6661 agaacttcat ccaccagcac ggcggggtca tcgaagtgga atccgagccc ggccgtacct
     6721 gcttcaccat cgtgctcccg ctcgaggccc gccgcgaggc gggcgagccc ccggaagcgc
     6781 gctagagcca accccatcac gccatgaaac ctgtctggat catcgacgac gaccgctcca
     6841 tccgctgggt gttcgagaag acgctcgccc gcgaggacat cccgttcaag accttctcct
     6901 cggcgcagga cgcgctcggc gcgctgcccg ccggcgcgcc gcaggtggtg gtcagcgaca
     6961 tccgcatgcc cggcggatcg ggcctcgagc tgctgcagaa gctcaaggag cgccacccgc
     7021 atctgccggt catcatcatg accgcctact ccgacctgga gagcgcggtg gccgcgttcc
     7081 agggcggcgc cttcgagtac ctgcccaagc cgttcgacgt ggatcacgcc gtggacctga
     7141 tccgccgcgc gatggaagag agcctgcgcc agggcgaagc cggcgaagtg tccgaggcga
     7201 tgccggagat cctgggccag gcggcctcca tgcaggaggt gttccgcgcc atcggccgtc
     7261 tgacccagtc gcacgccacc gtgctgatca acggcgagtc gggcaccggc aaggagctgg
     7321 tggcgcgggc gctacaccgc cacagcccgc gggccgcgaa gccattcatc gccatcaaca
     7381 ccgcggcgat ccccaaggag ctgctcgaat ccgagctgtt cggccacgag cgcggcgcct
     7441 tcaccggcgc gcagagcatg cgccgcggcc gcttcgagca ggccgagggc ggcaccctgt
     7501 tcctggacga gatcggcgac atgccggctg acctgcagac gcgactgctg cgcgtgctgt
     7561 ccgacagcca cttctaccgc gtcggcggcc accagccgat caaggcgaac gtgcgcatca
     7621 tcaccgccac gcatcaggac ctcgagagcc gcgtcaagca gggcctgttc cgcgaggatc
     7681 tgttccaccg gctgaacgtg atccgcgtgc gcctgcccgc gttgcgcgag cggcgcgagg
     7741 acatcccgct gctggcgcgc tacttcctcg gcaagagcgc gcgcgagctg ggcgtggagc
     7801 ccaagcgcct gaccgacgcc gccatgaaat acctgatgag ccacgacttc ccgggcaacg
     7861 tgcgccagct cgagaacctg tgccactggc tcaccgtgat ggcgcccggg gtgagcgtgg
     7921 acctgcacga cctgccgccg gaattccgcc aggatcagcg cgccatgacc acccagagct
     7981 ggatcgcggc gctggagcgc gaagccgagc tggcgctgaa ccgcggcgag cacagcatca
     8041 tgccggagct gtcgcgccag ttcgagcgcg ccctcatcgt caaggcgctg gcgcacaccg
     8101 gcgggcgccg aatcgaagcc tcgcacctgc tgggcatggg ccgcaacacg ctcacgcgca
     8161 agatccaaga gttggagatc gagtcgaagg acgcggcggg cgaatgaagc cgcggctcaa
     8221 ctctcgccga gttcggtgac caccggggcg tgatcggacg gccgctcctg cgcccgcacc
     8281 tgcttgtgga tggcgctggc cttcgcctgc ttcgccaggg gccgtgacag caggatgtgg
     8341 tcgatgcgca gacccatgtt gcgcctgaac tggttcatgc ggtagtccca ccagctgaac
     8401 gacctctcgg gctgctcgaa caggcgaaag ctgtccgcca gacccaggtc gaacaaacgg
     8461 cgcagcgcgt cgcgttcggg cgcgctgaac aacactttgc cctcccacag ccgcggatcg
     8521 tgcacgtcgc gctcctcggg cgcgacgttg aagtcgccca gcagtgcgag ccgcggatg
//
LOCUS       DASPGE010000114         2861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1438401, whole genome shotgun sequence.
ACCESSION   DASPGE010000114 DASPGE010000000
VERSION     DASPGE010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438401"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VD791_03640"
     CDS             <1..543
                     /locus_tag="VD791_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXV07095.1"
                     /translation="LCDISGSMNPYARMFLHFLHAITNDRDRVHTFVFGTRLTNVTRQ
                     LRHRDVDVAKTRVADAIKDWSGGTRIGVCLREFNWRWGRRVLGQNACVLLVSDGLDRE
                     AGEGLADEMQRLHKSCRTLIWLNPLLRYDRFEAKPAGVRAMLPHVDHFLPVHNLKSLV
                     ELAHALSRPANARMETRAWK"
     gene            540..1133
                     /locus_tag="VD791_03645"
     CDS             540..1133
                     /locus_tag="VD791_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HXV07096.1"
                     /translation="MSGEERIPASQADTWAALNDPEMLKACVPGCDAIERIGDDEFQV
                     LMVARIGPVSAKFKGKLTLSDLDPPNSYALAFEGQGGAAGFGKGSASVQLSPEGEGTL
                     LRYQVKASVGGKLAQIGSRLVDAAARKISQDFFAAFNEKVGALHGAGAAVGQEAEHHP
                     APLGRDADLPTVSDASLIFLAAGALVVLAVALYVLLA"
     gene            1282..1890
                     /locus_tag="VD791_03650"
     CDS             1282..1890
                     /locus_tag="VD791_03650"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXV07097.1"
                     /translation="MSGPNEHAAVRTESVILIAELRNFTRMSEMLEPERVLGLVDRFF
                     EMAARCVQAHGGLPMSVHNDSLVAAYYDGKPADMGRNAVRGAQQLFAEFDALAQEWER
                     DFGLRTAMSLGMHRGEAVYGEAGPRSERRQVVFGDCVSIAERLVHRARAGELVLSDAV
                     MSVLPVEELEMDAEPLPPLELHHRPAMRIYGLLRDERLDFTT"
     gene            complement(1880..2428)
                     /locus_tag="VD791_03655"
     CDS             complement(1880..2428)
                     /locus_tag="VD791_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV07098.1"
                     /translation="MRSLRLAAALVLLCGLPAGAADREREPDVIYVPTPQSVVEDMLR
                     LADVKKGDVLYDLGCGDGRIPVTAARKYGVRAVGIDIDPERIREANRNAKKSGVTRLV
                     RFRQQDLFQTDLREATVVTLYLLPDLNLKLRPRLLEQLKPGTRVVSHQFDMGDWKPDK
                     TLETGGATVYLWTVPPRKAARS"
     gene            complement(2482..2742)
                     /locus_tag="VD791_03660"
     CDS             complement(2482..2742)
                     /locus_tag="VD791_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07099.1"
                     /translation="MKPEIDREHPIAAWVGIVAILIAILLILWARIEIHGTHDLEQTL
                     SAEEPRNGPERLKPSPAADKGARGSEVDDAVATGRWATGFSP"
BASE COUNT          419 a          977 c         1016 g          449 t
ORIGIN      
        1 ctgtgcgaca tctccggctc gatgaacccg tacgcgcgca tgttcttgca cttcctgcac
       61 gccatcacca acgaccgcga ccgggtgcac accttcgtgt tcggtacgcg cctcaccaac
      121 gtcacccgcc agctccggca ccgcgacgtc gacgtcgcca agacgcgggt ggccgacgcc
      181 atcaaggact ggtcgggcgg cacgcgcatc ggcgtgtgcc tgcgcgagtt caactggcgc
      241 tgggggcggc gcgtcctcgg ccagaacgcc tgcgtgctgc tggtctccga cggcctggac
      301 cgggaggcgg gcgaaggcct ggccgatgag atgcagcgcc tgcacaagtc ctgccggacg
      361 ctcatctggc tgaatccgct cctgcgctac gaccgcttcg aggccaagcc cgcgggcgtg
      421 cgcgccatgc tgccgcacgt ggatcatttc ctgccggtgc ataatctgaa gagcctggtc
      481 gagctggcgc acgcgctcag caggccggcg aacgcacgca tggagacccg agcatggaaa
      541 tgagcggcga agagcgcatc ccggcatcgc aggccgatac ctgggcggcg ctcaacgatc
      601 ccgagatgct caaggcctgc gtgccgggct gcgacgccat cgagcgcatc ggcgacgacg
      661 agttccaggt gctgatggtg gcgcgcatcg ggccggtgag cgccaagttc aagggcaagc
      721 tcacgctgtc cgacctcgac ccgcccaact cgtatgcgct cgctttcgag ggccagggcg
      781 gcgcggccgg gttcggcaag ggctcggcca gcgtgcagct ctcccccgag ggcgaaggca
      841 cgctgctgcg ctaccaggtg aaggcgagcg tcggcggcaa gctggcgcag atcggctcgc
      901 gcctggtgga cgccgcggcg cgcaagatct cgcaggactt cttcgccgcg ttcaacgaga
      961 aggtcggcgc gctgcacggc gccggcgcgg ccgtggggca ggaggccgag caccacccgg
     1021 ctccgctcgg gcgtgatgcg gacctgccga cggtgtccga cgcctcgctc atctttctcg
     1081 ccgccggggc gctcgtggtg ctcgcggtgg cgctgtacgt cctgctcgcc tgatgggctc
     1141 cgcccgtccc ggcgggccgg ccggccgtcc gggggcacgg gccggcgagg tattatttcc
     1201 tcctcgtgcc gcgcgggcgc tgactagcat gcgcgcgtcg ctttgcgcga aagtccaccc
     1261 agggggggag gcttagctgt cgtgtcgggg ccgaacgagc atgcggcggt gcgcaccgaa
     1321 tcggtgatcc tgatcgccga gctgcgcaat ttcacgcgca tgtcggaaat gctcgagccg
     1381 gagcgcgtgc tcgggctggt cgaccggttc ttcgaaatgg cggcgcgctg cgtgcaggcg
     1441 cacggcggcc tgcccatgtc ggtgcacaac gattccctgg tggcggccta ctacgacggc
     1501 aagcccgcgg acatggggcg caacgccgtg cgcggcgccc agcagctgtt cgccgagttc
     1561 gacgcgctgg cgcaggaatg ggagcgcgat ttcggcctgc gcaccgcgat gtcgctcggc
     1621 atgcatcggg gagaggcggt gtacggggaa gcgggtccga ggagcgagcg gcgccaggtg
     1681 gtgttcggcg actgcgtgag catcgccgag cggctggtgc accgcgcgcg cgcgggcgag
     1741 ctggtgctgt ccgacgccgt catgagcgta ttgccggtcg aggagctgga gatggacgcc
     1801 gagccgctgc cgccgctcga gctgcaccac cggcccgcca tgcgcatcta cggcctgctg
     1861 cgcgacgagc ggctcgactt cacgacctag ccgccttgcg cggcggcacg gtccacaggt
     1921 agaccgtggc gccgcccgtc tccagcgtct tgtccggctt ccagtcgccc atgtcgaact
     1981 ggtgcgagac gacgcgcgtg cccggcttga gctgctcgag cagccgcggc cgcagcttga
     2041 ggttgaggtc gggcagcagg tagagcgtga ccaccgtcgc ttcgcgcagg tcggtctgga
     2101 acaggtcctg ctgccggaag cgcaccagcc tggtgacgcc gctcttcttc gcgttccggt
     2161 tggcctcgcg aatgcgctcg ggatcgatgt cgatgccgac tgcgcgcacg ccgtacttgc
     2221 gggccgcggt caccgggatc ctgccgtctc cgcagcccag gtcgtacagc acgtcgccct
     2281 tcttcacgtc ggcgagccgc agcatgtcct cgacgacgct ctgcggcgtg ggcacgtaga
     2341 tgacgtcggg ctcgcgctca cggtcggccg cgccggcggg caggccgcac aacaggacaa
     2401 gcgccgccgc caggcggagg gaccgcatgc aaactcctcg caggatcgcg cccggggatt
     2461 atctcccagg cgagcgggcg ctcagggcga gaaccccgtg gcccagcgac cggtcgccac
     2521 ggcgtcgtcg acttccgagc cgcgggcgcc cttgtcggcg gccggcgacg gcttgagccg
     2581 ctccggccca ttgcggggct cttcggccga gagggtctgc tcgaggtcgt gcgtcccgtg
     2641 gatttcgatt ctcgcccaaa gaatcaacag gatagcgatc agtatggcga cgatgcccac
     2701 ccaggcggcg atgggatgct cgcgatcgat ctcgggcttc aaagcagggt ccatgatgag
     2761 cagtacgcaa agcctatgcc cgttcgaccg ccacgcctat cccaaggttc tggccggcta
     2821 ctccccagca atggtcatgt tctgcaccag gatcgagccg c
//
LOCUS       DASPGE010000115         2671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_502128, whole genome shotgun sequence.
ACCESSION   DASPGE010000115 DASPGE010000000
VERSION     DASPGE010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502128"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /gene="groEL"
                     /locus_tag="VD791_03665"
     CDS             complement(<1..850)
                     /gene="groEL"
                     /locus_tag="VD791_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HXV07100.1"
                     /translation="MPAKQLLFHDSAWAKILIGVNTLADAVKVTLGPKSRNVILQQPF
                     GAPSVANSGVVVAKAIELQDPFENMGAQMVREVAAKTSEVAGDGTTTATVLAQGIVRE
                     GMKYVAAGMSPMDLKRGIDRAVAALVAELKKASRPCRTSEEITQVGAISANNDKSVGR
                     IIAQAMDKVGREGVITVEDGSGLENELETVEGMQFDRGYLSPYFINSAEKRTCILDDP
                     YILVYEKKIGTVAELLPILEEITRVNRPLLIVAEDVEGEALATLVVNAVRGVLKACAV
                     KAPGFGD"
     gene            complement(869..1120)
                     /locus_tag="VD791_03670"
     CDS             complement(869..1120)
                     /locus_tag="VD791_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXV07101.1"
                     /translation="MDQADIRRALIDALASVAPEGDYDRLKPDRPLRDQLDIDSYDFL
                     NVVVHLHEQLGVDIPEADYQKLATLDSAVAYLAPRLGAA"
     gene            complement(1120..2298)
                     /locus_tag="VD791_03675"
     CDS             complement(1120..2298)
                     /locus_tag="VD791_03675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HXV07102.1"
                     /translation="MAEFRMPSLGADMESGVLVEWLVAPGQAVKRGQVVAVVETQKGA
                     VEVEIWEAGVVDALVVRPGTRVPVGEVLATLRDGEARPAAVPPSLPPTPPPAPAVQPV
                     PEPFVPAAAAHVKASPAARKRADALRVDLARVTGSGPGGAITLEDVESAAARAAQAPA
                     PPAVRAAAPADKQAAMRAAIAAAMSKSKREIPHYYLGTTIDVTRAQDWLTQENAKRPV
                     KERILFAAVLLKAVAGALPEVPELNGFWVDGAFRPSAAVHLGVGIALRGGGLIAPAIH
                     DANSKSVAELMAALGDLIQRARTGALRSSELTDATVTVTNLGEQGVESVYGVIYPPQV
                     ALIGLGKIVERPWAEDGGVFVRRVLHATLSADHRASVGHRGGLFLAALDRLLQQPEKL
                     "
     gene            complement(2291..>2671)
                     /locus_tag="VD791_03680"
     CDS             complement(2291..>2671)
                     /locus_tag="VD791_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXV07103.1"
                     /translation="GKDVSLITYGGSLGKALQAAAQLAQDGIDAEVVDLRSLRPLDEA
                     TFLASVAKTHRAVVVDEGWKSVGLSAEIVARIMEGVFYELDAPVARVCSREVPVPYAR
                     HMEEATLPQVARIVAAAKQVLGRG"
BASE COUNT          379 a          953 c          898 g          441 t
ORIGIN      
        1 ggtcgccgaa gcccggcgcc ttgaccgcgc aggctttgag cacgccgcgt acggcgttga
       61 ccaccagcgt ggcgagcgct tcgccttcca cgtcctcggc gacgatcagc aggggccggt
      121 tgacgcgcgt gatctcttcc aagatcggta gcagctcggc caccgtgccg atcttcttct
      181 cgtacaccag gatatagggg tcgtccagga tgcaggtacg cttctcggcg ctgttgatga
      241 agtacggcga gagatagccg cggtcgaact gcatgccctc gacggtctcc agctcgttct
      301 ccaggccgga gccgtcttcc acggtgatca ccccttcgcg cccgaccttg tccatggcct
      361 gcgcgatgat gcggccgacc gacttgtcgt tgttggcgga gatggcgccg acctgcgtga
      421 tctcctcgct cgtgcggcaa ggtctggatg ccttcttcag ctccgccacc agcgcggcca
      481 cggcgcggtc gatgccgcgc ttcaggtcca tcgggctcat gccggcggcc acgtacttca
      541 tgccttcgcg cacgatgcct tgggcgagca ccgtcgcggt ggtggtgccg tcgcccgcga
      601 cctcggaggt cttcgcggcc acctcgcgca ccatctgcgc acccatgttt tcgaatgggt
      661 cctgcagctc gatggccttg gccaccacca cgccggagtt ggccacgctg ggcgcgccga
      721 agggctgctg gaggatgacg ttgcggctct tcgggccgag cgtgaccttg accgcgtcgg
      781 cgagcgtatt cacgccgatg aggatcttgg cccaggcgct gtcgtggaag aggagctgct
      841 tggctggcat ggttgacctc tgattgggtt acgcggcgcc cagccgcggc gccaggtagg
      901 ccaccgcgct gtcgagggtg gcgagtttct ggtagtccgc ctcggggatg tccacgccga
      961 gctgctcgtg cagatgcacg accacgttca ggaaatcgta ggagtcgatg tcgagctgat
     1021 cgcggagcgg gcgatccggc ttgagccgat cgtagtcgcc ctcgggagcg acgctggcca
     1081 gtgcgtcgat cagcgcgcgg cggatgtctg cctggtccat cagagtttct ccggttgctg
     1141 cagcaggcgg tcgagggcgg cgaggaacag accgccgcga tgcccgacgc tggcgcggtg
     1201 gtccgcggac agggtggcat gcagcacgcg ccgcacgaac acgccgccgt cctcggccca
     1261 ggggcgctcg acgatcttgc ccaggccgat cagcgccacc tgcggcggat agatcacgcc
     1321 gtagacgctt tccacgccct gctcgccgag attggtgacg gtgacagtgg cgtcggtcag
     1381 ctcggagctg cgcagcgcgc cggtgcgggc gcgctggatc aggtcgccaa gcgccgccat
     1441 cagctcggcc accgacttgc tgttggcgtc gtgaatcgcc ggcgcgatca gacccccgcc
     1501 gcgcagcgcg atgcctacgc ccaggtgcac ggccgcggat ggccggaacg cgccgtcgac
     1561 ccagaagcca ttgagctcgg gaacttctgg cagcgcgccg gcgaccgcct tcagcagcac
     1621 ggcggcaaac aggatgcgct ccttcaccgg gcgcttggcg ttctcctgcg tgagccagtc
     1681 ctgcgcgcgc gtgacgtcga tcgtcgtgcc caggtaatag tgcgggatct cgcgcttgga
     1741 tttggacatg gccgcagcaa tggcggcgcg catcgccgcc tgcttgtcgg cgggcgccgc
     1801 tgcgcgaaca gcaggcgggg ccggcgcttg cgctgcacgg gcagccgcgc tctctacgtc
     1861 ctcgagcgtg atcgcgccac cggggccgct gccggtcacg cgcgccagat cgacccgcag
     1921 cgcgtccgcg cgcttgcgcg ccgcgggcga tgccttcacg tgcgcggcag cggctggaac
     1981 gaacggctcg ggcacgggct gtaccgcagg cgccggcgga ggcgttggcg gaagacttgg
     2041 cggcacagcc gcgggacgtg cttcgccgtc acgcagcgtg gcgagcacct cacccaccgg
     2101 cacccgcgtt ccgggccgta ccaccagcgc atccaccacg ccggcttccc agatctccac
     2161 ctcgaccgcg cctttctgcg tttcgaccac cgccaccacc tgcccgcgct tgactgcttg
     2221 gcccggcgcg accagccact ccaccagcac gcccgactcc atgtcggcgc ccagcgaagg
     2281 catgcggaac tcagccacgg cccagcacct gcttcgcggc ggccacgatg cgcgcaactt
     2341 gcgggagggt ggcctcctcc atgtggcgcg catagggtac cggcacctcg cggctgcaca
     2401 cgcgcgccac cggcgcatcc agctcgtaga aaacgccttc catgatccgt gcaacgatct
     2461 ctgccgacag gcccacgctc ttccagccct cgtcgaccac caccgcgcgg tgcgtcttgg
     2521 ccaccgacgc caggaaagtc gcctcgtcga gcgggcgcag gctgcgcagg tccaccacct
     2581 ccgcatcgat gccgtcctgc gcgagctgcg cggccgcctg cagcgccttg cccaggctgc
     2641 cgccgtaggt gatcaggctc acgtccttgc c
//
LOCUS       DASPGE010000116         4327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_268830, whole genome shotgun sequence.
ACCESSION   DASPGE010000116 DASPGE010000000
VERSION     DASPGE010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268830"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..515)
                     /locus_tag="VD791_03685"
     CDS             complement(159..515)
                     /locus_tag="VD791_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07104.1"
                     /translation="MAGFFLRAAFLFLPAGFFAAFFAPAFLLEGRLAFFAAGFFAAFF
                     FAVFFLARFARLAGAFLAADLRGALRRAGRLAAIFFMAGRFADFLRALRAGAFFLAAD
                     FFAADRFIAIVAYSSG"
     gene            686..898
                     /locus_tag="VD791_03690"
     CDS             686..898
                     /locus_tag="VD791_03690"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HXV07105.1"
                     /translation="MDRKKVNSSGIRSIGYEASSQTLEVELADGSIWQYSKVPSEVHR
                     RLMAAPSMVSYYRDNVEEEYSRRRLK"
     gene            complement(900..1493)
                     /locus_tag="VD791_03695"
     CDS             complement(900..1493)
                     /locus_tag="VD791_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HXV07106.1"
                     /translation="MDDSVARALAKWPNVPAAYGWLALDRRGNWSIKGERIGNPALVD
                     FIGRNYEHDARGRWFFQNGPQRVFVRLEYTPYVLRTEPAASGLALRTHSGMPVRPRAA
                     WLDDAGSLLVEFGDSVGVVHGQDLAQMLSCLRDARGDELSDDALEALLQAGSRALAVV
                     LAAAARTVPVGRIAAAEVAARFGFDPDPRPAPGEPEC"
     gene            complement(1724..3052)
                     /locus_tag="VD791_03700"
     CDS             complement(1724..3052)
                     /locus_tag="VD791_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HXV07107.1"
                     /translation="MTGLHPAALLVLYGALALAPLALAAWSGPRASDFLAELGTGTAL
                     VAYAMLLLQFISSGRFERLSGRAGIDRTMRFHQLGARVAALLVIAHPLIPRLPTQVDQ
                     IGMLPAAMAIMFRAPHLLSGVVAWVLVLALVAMAVLRRRLPLPYEAWRATHALGAVTI
                     ALAGAHHTFSVGAYSREPALFWFWCALLCGALASMVFVYGVRPALQSRAGYRITQNRA
                     IGAGIRELTLDPLPGRGIRPRAGQFAWINLRRPPLPVFDHPFSFSSAPAEAPRLRLTI
                     KARGDFTSRLHALAPGAPVVVDGPHGSFGLQDRRADAVCLVAGGIGIAPIISVMRDLH
                     AGADPRPVALLYGARNLAQLVYAEEIRAMRGRLRLHTAFHLDEPPSGWIGGVGPINAA
                     AFRAALAPVPAARWVCLVCGPTPMVLAAERHLIAGGVPSDRIVYERFEYD"
     gene            3142..>4327
                     /gene="otnK"
                     /locus_tag="VD791_03705"
     CDS             3142..>4327
                     /gene="otnK"
                     /locus_tag="VD791_03705"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HXV07108.1"
                     /translation="MAGPLLLGCIADDFTGATDLANTLVRAGMRTIQLLGVPRPGLEM
                     PQADAVIVALKSRSNPAAEAVASSLAALAWLRANGARQFYFKYCSTFDSTDRGNIGPV
                     ADALLDALSADFTVFNPAFPTNKRTVYKGYLFVGDELLSESGMRHHPLTPMTDPSLTR
                     VLQRQTRHKVGLVPYATVVQGAPAVREAFAELRRSGVRHAILDSITDQHLHTLGEACA
                     DLRLVTGGSGMAMGLPANFVHAGLLQAGRHAVLPRVTGPTVVLAGSCSVATQGQVAKM
                     KQDHEAFELDPIAIAGGKDVAAQALAWASTRLGDKPILLYSTAAPERVAAVQSQLGRE
                     EAGALIERALGRIAQGLVQQGVRRLVVAGGETSGAVVSALGVEGLAIGPEIDPGVPWT
                     FSI"
BASE COUNT          738 a         1484 c         1542 g          563 t
ORIGIN      
        1 ctttccgtgc cggcacggaa agcggccggc ccctttttgc cgtccgcgcg gcagcggacg
       61 gccagaggaa ctgcgtcacc ggacgccagg ccgccagtag gggccgaagc gtccggcagc
      121 gtggtcgcac cgcgaggtgc gtgcagtacg tgttcaactc aacctgagga gtaagccaca
      181 atggctatga aacgatctgc agcgaagaag tccgcggcca agaagaaggc cccggcgcgt
      241 aaggcccgca ggaaatcggc gaagcgtccg gccatgaaga agatggccgc caagcgcccg
      301 gcccggcgca gagcgccgcg caagtcggcg gccaagaagg ccccggccaa gcgcgcgaag
      361 cgcgccaaga agaagacggc gaagaagaag gcggcgaaga aaccggcagc gaaaaaagcc
      421 aagcgacctt cgagaaggaa agcgggcgca aagaaggcag cgaagaaacc tgctggcaag
      481 aagaggaagg cagctcgcaa gaagaagcct gccatggccg aagccgctgc cccggcagca
      541 ccgaagcccg ctcccgcgcc ggcagcttgg ccgttcccgg tgtctggtaa ccggccgaac
      601 taagctgtag cttccaggag gctcgcggga ggcctgcatc cgtgaggata ccagtcacct
      661 ccgcgggctt tgtgttccgg ttgcgatgga ccgcaagaag gtcaattcga gcggcatccg
      721 ctcgatcggc tacgaggctt cctcccagac cctcgaggtc gagctcgccg acggcagcat
      781 ctggcagtac tccaaggtgc cctccgaggt gcaccggcgc ctgatggccg cgccctcgat
      841 ggtcagctac taccgcgaca acgtcgagga agagtacagc cgccgacggc tgaaataggt
      901 cagcattccg gctctcccgg cgccggccgc ggatccggat cgaagccgaa gcgggccgcc
      961 acctcggccg ctgcgatgcg gccgacgggc actgtccggg cggctgccgc cagcaccacc
     1021 gccagcgccc tcgagccggc ttgcagcagc gcctccagcg catcgtccga aagctcatca
     1081 ccgcgcgcgt cccggagaca ggacagcatc tgcgccaggt cctggccgtg caccacgccg
     1141 acgctgtcgc cgaactcgac cagcaggctt cccgcatcgt ccagccaggc ggcgcgcggc
     1201 cgcaccggca ttccgctatg ggtgcgcagt gccaggccgg acgctgcagg ctccgtgcgc
     1261 aacacgtagg gcgtgtattc caggcgcacg aacacgcgct gcgggccgtt ctggaagaac
     1321 catcttcccc gcgcgtcatg ctcgtagttg cggccgatga agtccaccag cgccgggttg
     1381 ccgatgcgct cgcccttgat cgaccagttg ccgcgccggt cgagcgccag ccagccgtag
     1441 gcggcgggaa cgttgggcca cttggccagg gcgcgagcta cgctgtcgtc catgtgcgct
     1501 tccagcgcaa tgcgggtgcg ctgcgcccta cagtttagta gcatgcgccg gaccgcattt
     1561 ccagcagggt tggaccgggc cgacggcgcc gctgcggcac cggccacacg cgggcggcgg
     1621 ccagtcaggg cccgctagcg cagcgcgaaa gcgagcaggg ccgccacgat cacggcgttg
     1681 accgccagga acaccagcag gaagcgcgtc tcccgcgtca tcgtcagtcg tactcgaagc
     1741 gctcgtagac gatccggtcc gagggcacgc cgccggcgat gaggtggcgc tcggcggcga
     1801 gcaccatcgg cgtgggtcca cagaccaggc atacccagcg cgctgcaggg accggtgcga
     1861 gcgcggcgcg gaatgccgcg gcgttgatcg gcccgacccc gccgatccac ccggacggcg
     1921 gttcgtccag atggaatgcc gtgtgcagcc gaaggcggcc gcgcatcgca cggatctctt
     1981 cggcgtacac cagctgcgcg agattgcggg cgccgtagag cagcgcaacc ggccgcggat
     2041 cggcgcccgc atgcaggtcg cgcatcacgc tgatgatcgg tgcgatgccg atgccgcctg
     2101 ctaccaggca caccgcgtcg gcgcgacgat cctgcagtcc gaagctgccg tgcgggccgt
     2161 ccacgaccac gggcgcgccc ggggcgaggg cgtgcagccg gctcgtgaag tcccccctgg
     2221 ccttgatcgt caggcgcagc cgcggggcct ccgccggtgc ggagctgaac gagaacgggt
     2281 gatcgaatac gggcaacggc gggcgcctca gattgatcca tgcgaactgg ccggcgcgcg
     2341 ggcggatgcc gcggccgggc agggggtcga gcgtcagctc gcggatgccg gcgccgatcg
     2401 cacggttctg cgtgatgcga tagcccgccc gcgactgcag cgccgggcgc acgccgtaca
     2461 cgaacaccat cgatgcgagc gccccgcaca gcagggcgca ccagaaccag aacaacgccg
     2521 gctcccggct gtacgcgccg acgctgaaag tgtgatgcgc cccggccagg gcgatggtca
     2581 ccgcgccgag cgcatgggtc gcgcgccacg cttcataggg cagaggcagg cgccgccgca
     2641 gcaccgccat cgcgaccagc gccagcacca gcacccaggc caccacgccg ctcagcaggt
     2701 gcggcgcgcg gaacatgatg gccatcgctg cgggaagcat gccgatctgg tcgacctgcg
     2761 tgggcaggcg cgggatcagg gggtgcgcga tcaccagcag cgccgccacc cgtgctccga
     2821 gctgatggaa gcgcatggtg cggtcgatgc ctgcgcgccc ggacaggcgc tcgaagcggc
     2881 ccgacgaaat gaactgcagc agcagcatcg cgtaggcgac cagcgcggtg ccggtgccga
     2941 gctcggccag aaagtcgctc gcgcgcgggc cgctccaggc cgcgagcgcg agcggcgcca
     3001 gcgccagcgc gccgtagagg accagcagcg ccgccggatg caggccggtc acgtgcggga
     3061 ggtggtctgg ctcggcatgc gatggggccg cgggtttttc gctaccatta tgccaaggcg
     3121 catcgacggg aggaacagtt cgtggcaggg ccgcttctgc tcggttgcat cgcggacgat
     3181 ttcaccggcg cgacggatct cgccaacacg ctggtgaggg ccggcatgcg caccatccaa
     3241 ctgctcggcg tgccgcgccc aggtctcgag atgccgcagg ccgacgccgt gatcgtcgcg
     3301 ctcaaatcgc gcagcaaccc cgccgccgag gcggtggcga gctcgctggc ggcgctcgcg
     3361 tggctgcgtg ccaacggcgc gcgccagttc tacttcaagt attgctcgac cttcgattcc
     3421 accgaccgcg gcaacatcgg tccggtggcc gacgccctgc tggacgcgct gagcgcggac
     3481 ttcacggtgt tcaatcccgc cttcccgacc aacaagcgca cggtgtacaa gggctatctg
     3541 ttcgtgggcg acgagctgtt gtccgagtcg ggcatgcgcc atcatccgct cacgccgatg
     3601 accgatccgt cgctcacgcg cgtgctgcag cgacagacgc ggcacaaggt ggggctggtg
     3661 ccctacgcca cggtggtgca gggcgcgccg gcggtgcgcg aggccttcgc cgagctgcgc
     3721 cgcagcgggg tgcgccacgc catcctagac tcgatcaccg accagcacct gcacacgctg
     3781 ggtgaagcgt gtgcggacct gagacttgtc accggcggct ccggcatggc gatgggcctg
     3841 cccgccaact tcgtacacgc ggggctgctg caggccggtc ggcatgcggt gctacccagg
     3901 gtcaccggcc ctacggtcgt gctggcgggc agctgctcgg tggccacgca gggccaggtg
     3961 gcgaagatga agcaggacca cgaggccttc gagctggacc cgatcgccat cgccggcggc
     4021 aaggacgtgg ccgcgcaggc gctggcgtgg gcctccacgc ggctcggtga taaaccgatt
     4081 ctgttgtact ccaccgccgc gcccgagcgg gtggcagcgg tgcagagtca gctgggacgg
     4141 gaagaggcgg gcgcgctgat cgagcgcgcg ctgggccgca tcgcgcaggg cctggtgcag
     4201 cagggggtgc gccggctggt ggtggccggc ggcgagactt ccggtgcggt ggtgagcgca
     4261 ctcggggtgg aaggactggc catcggcccc gagatcgacc cgggcgtgcc ctggaccttc
     4321 agcatcg
//
LOCUS       DASPGE010000117         8794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1205681, whole genome shotgun sequence.
ACCESSION   DASPGE010000117 DASPGE010000000
VERSION     DASPGE010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8794
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205681"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..838)
                     /gene="bamD"
                     /locus_tag="VD791_03710"
     CDS             complement(113..838)
                     /gene="bamD"
                     /locus_tag="VD791_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HXV07109.1"
                     /translation="MSGRPALARAGVLLACLAAPAPAHDLITGEAAQRYLAQAVEQLA
                     LLDARQPAAQRAQASYALGRMLDQIRDLLNRDLATHGRVQGLPTQRLMAELDARGLRL
                     SVSPQLKRFPANLDYYREALRLAPDGPRAADAAFALLQGYFYDSFADDPLQPRDQSWG
                     RLAEQIAIGERFLERAPAHPEREEAEFILVVHYVQAARSAPDATTRTRYAQRARQAAE
                     RFAARYPDSLRAAALPVLLEDLP"
     gene            complement(840..1388)
                     /locus_tag="VD791_03715"
     CDS             complement(840..1388)
                     /locus_tag="VD791_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07110.1"
                     /translation="MQRRRFLASLAAAGGVWLLSGHSPYRQWKLYRQSHLLLFTTRDD
                     EGSDALGEALAARLREALPDSRAGVGRAPNLQRLASLLSSAQADVAVLKMENALALYR
                     GAPPFADFAPMPLRVLVETQGYQLVCRDDFKERHAYLVAEALTREPRAAPVRVPEREG
                     DEAGVPTHAGALAFLRGRALDD"
     gene            complement(1438..2694)
                     /locus_tag="VD791_03720"
     CDS             complement(1438..2694)
                     /locus_tag="VD791_03720"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HXV07111.1"
                     /translation="MKARFAALCAALAAASLAVPAGADTTDVKTQSWVHVKKTGAHCA
                     DDPNCINRYHPAIKPAARARPGQLIVFETRDALDSDLTVNSTAKDMAALDLNLVHPIT
                     GPVHIEGAMRGDVLAVTLVDVDPDEYGYTLIVPGFGFLRDLFTEPFIANWKLNRMEAV
                     SDQIPGVAIPFNGFMGTVGTMPGFPETDAWFAREKQLGEAGGIALPPQPTGALPAAVC
                     GPNGSHKDKCLRTIPPRENGGNMDIKQMVVGTTLLLPCYIDGCGLIVGDVHYAQGDGE
                     VSGTAIEIGAKVTVRTEIRKGMAAMLKSGPHFEGGSQLKKLEPDKFYATVGYPLKKAG
                     EVPPQHKYLDSAKLPALTNLNEDLVVAARNSLNEMIDWLVKTRGYTRQQAYIFTSVAC
                     DLRIGNVVDVPNYAVSTVCPLEVFKK"
     gene            complement(2917..4008)
                     /locus_tag="VD791_03725"
     CDS             complement(2917..4008)
                     /locus_tag="VD791_03725"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HXV07112.1"
                     /translation="MFRRLRTFAAFLTASACLLAAGSAWPHATQLSSSRAQVHGGRID
                     VLLELNALDVQVALEVALLGADGQVAPAALREVEQQLGAYILERAKLSNTAGAACHGR
                     LAGARPKAEHVLVDIEWSCPPMTGQLIYEVTLFQEVDPAARHMVTAGGDVRRMALLST
                     GNPRVALSDTRPHVGEVMWHYLLAGIEHIAIGYDHIAFLVAVILWGRRVWPLVGVVTA
                     FTVAHSITLTLAVLDVVRLPSQLVETAIALSIVYVAAENFFVRDMRRRAWITFAFGLI
                     HGFGFASVLRDYGIPRDALVPALAAFNVGVEVGQIVIVVAALGLMRGVERALRGPFPA
                     FPTAYPYAVSASVLLLGLYWTVERLLPGA"
     gene            complement(4136..7861)
                     /locus_tag="VD791_03730"
     CDS             complement(4136..7861)
                     /locus_tag="VD791_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07113.1"
                     /translation="MAAEVLPQTMRFHIGFFWGLPLSVLGFAALAAGAGYLLLTDYHG
                     HLWLRCAVHAVGGALLGVAAWRASDRLTDYVNLHTSPLLRLALAGTGATWIATEVAGV
                     FTELAVGSGAWWAEAAAPLLFFAVGVAPLLAGIALLAGIETLLAAWRGDGGAFRAVRP
                     IAVHMLVAVLVLGAVGYVAVMLPDAPIRAAMALAPERARTSGWVQRESSLLTEAHAGW
                     SCEVLVVPVAGAAPSLDRAARSLITRLTAAELARRSGLCVADPTLVARALGAQDRGSA
                     QRAVRKLANALNARYLVQGEVRMDEQRPAFHVALQLYTRTQAQAPWEAGGEAVWGPLP
                     VSDELPPEAAFARIAREAAEALGIPLTGEDAQQAQADEPGRLPGTTSHLAGYGATPLA
                     RARALQLLAAAHHASELNGEHLWERSIVALSVLPATHGQARVLRARAALHLQRRPYAL
                     QLLDGLGGSEPAAVRALADGNLLEAERRRAELADAAALTLDLELAALRAHYGKHRANS
                     ERRQRLLDSWPGYAALLYVPMSPGDAATGDAHAVVAAQLAALGADAEPPGIAALAARV
                     GIKVPGRGAGLALAADLERGRAALWRANAGAWRAERGFDRVAAWDIPEALYAANRAAV
                     VLRLWALAAAPDSDQQLLDDLRALGSGFGGHAGIQAARAVVLQRLGAGGGERAFLIAE
                     RSRRLQADVLAWEGGESDTERELRARLQPVQPPQADEPPRPWRAVPAAEGEAGASALA
                     GAMHYARMQSYAQEDFAVLQRAIQAYERARQPDRVAQLMQLARTRFVGSPVRERFLLQ
                     RAEAERDLAAAVVLLLERIREQPEQWAAYPLLAKAYLRAREPAMAQRALLSYPPFNGA
                     GARGAVRQAAESGALLLAAGEGEFARPLLELAARAAEDGSEPSPRLEARMRLAQLDGD
                     WSEVRTLALALHEQHGAEHALARAGTLAFLLGEREAGWRAFYEASKRFETLAPWSAAV
                     DGHRLEATEPDEVVAFAKRWKSLSGERARETRLKERFLFNMLLVDRPGADWAVDALSS
                     FAREREDPAFAALVRGYAAFKRGDYAKVVGELEPLQQGGQEPETLPWLVLALYGTGRG
                     GDAQALLQAAGKQPGSAFYTTLAAAYAAGARGEHERALGALWDAFLAAPGHLDSAVPA
                     GYQVLEACERLLALSADERYRQLLLDLARRQQRAWPVSWAFAFEVRYATHADDAERAL
                     GAALYLDPESERLRHIAHPQRQRAAERFGRINPFRRA"
     gene            complement(7929..>8794)
                     /locus_tag="VD791_03735"
     CDS             complement(7929..>8794)
                     /locus_tag="VD791_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXV07114.1"
                     /translation="LLKQRPVLGGYDAARYASELTYATAADGERIPVSLVYRRELRAS
                     GPQAMLLTGYGAYGFPHDVRFSSARLSLLDRGVVVATAHVRGGGEMGKRWHDGGRMLN
                     KRNTFTDFVAAAEYLIARGYTRPDRLVIEGGSAGGLLMGAVTNARPDLFKAVVADVPF
                     LDLINTMLDPRLPLTTGEYEEWGDPRDRRYFEYMLSYSPYDNIEAKAYPAMLVQTSLN
                     DSQVMVWEPAKYVARLRATKTDTRPLLLKINMEAGHGGASGRYDFLREIAFTYAFVLW
                     QLERLPGVLEA"
BASE COUNT         1300 a         3190 c         3123 g         1181 t
ORIGIN      
        1 ggtaagagga cttcgcgcac gacggcgtag tgcggatgcc gccgccccga tacgtcgcct
       61 aacaaatggg cggcggaatc cggggaagag attccgcttc gctgcatgcg ggctacggga
      121 ggtcctccag cagcaccggc agcgccgcgg cgcgcaggct gtcgggatag cgtgccgcga
      181 aacgctccgc cgcctgccgc gcgcgctgcg cgtagcgcgt gcgcgtcgtc gcgtccgggg
      241 cgctgcgcgc ggcctggacg taatgcacca ccaagatgaa ttcggcttcc tcgcgctcgg
      301 gatgcgcggg cgcgcgctcg aggaagcgct cgccaatcgc gatctgctcc gcgagccgac
      361 cccagctctg gtcgcgcggc tgcagcgggt cgtcggcgaa gctgtcgtag aaatacccct
      421 gcagcagggc gaaggcggcg tcggccgcgc gcgggccgtc gggcgcaagc cgcagcgcct
      481 cgcggtagta gtcgaggttc gctggaaagc gcttgagctg cggcgacacg ctcagccgca
      541 atccgcgtgc atccagctcc gccatcaacc gctgcgtggg cagcccctgc acgcggccgt
      601 gggtggcaag gtcgcggttg agcaggtcgc ggatctggtc gagcatgcgg cccagcgcgt
      661 agctggcctg ggcgcgttgc gccgccggct gtcgcgcgtc gagcagcgcg agctgctcga
      721 cggcctgcgc caggtagcgc tgcgcggcct cgcccgtgat cagatcgtgg gccggcgccg
      781 gcgcggccag acacgcgagg agaacgccgg cacgagccag cgcgggccgg ccgctcacat
      841 caatcgtcga gggcccggcc gcgcaggaaa gcgagcgcgc cggcatgcgt cggcacgccc
      901 gcctcgtcgc cctcgcgctc cggcacgcgg accggcgcgg ctcgcggctc gcgggtgagc
      961 gcctcggcca ccaggtacgc gtgccgctcc ttgaagtcgt cgcggcacac cagctgatag
     1021 ccctgcgttt ccaccagtac acgcaaaggc atgggtgcga agtcggcgaa cggcggtgca
     1081 ccgcgataca gcgccagcgc gttttccatt ttcaggacgg ccacgtcggc ctgggcgctg
     1141 gacagcaggc tcgccagccg ctgcaggttc ggcgcgcgcc ccacgcccgc gcggctatcg
     1201 ggcagcgcct cgcgcaggcg cgcggcgagc gcctcaccga gcgcatcgga gccctcatcg
     1261 tcgcgcgtgg tgaacagcag cagatgcgat tgccggtaca gcttccactg ccggtagggc
     1321 gagtgcccgc tcagcagcca cacgccgccg gcggcggcga gcgatgcgag gaagcggcgg
     1381 cgctgcatgc agatcttccg gccctctccc gcgcgtgcgg gagagggcgc tgcgatgcta
     1441 cttcttgaag acctccagcg gacacacggt ggacacggcg tagttcggca cgtccaccac
     1501 gttgccgatg cgcaggtcgc aggccacgct ggtgaagatg taggcctgct ggcgcgtgta
     1561 gcccctggtc ttgaccagcc agtcgatcat ctcgttgagc gagttgcgcg cggccacgac
     1621 caggtcctcg ttcaggttgg tgagcgcggg cagcttggcg ctgtcgaggt acttgtgctg
     1681 cggcggcact tccccggcct tcttgagcgg atagcccacg gtggcgtaga acttgtcggg
     1741 ctcgagcttc ttcagctgcg acccgccctc gaagtgcggg ccggacttga gcatggccgc
     1801 catgcccttg cggatctcgg tgcgcaccgt gaccttggcg ccgatctcga tcgccgtgcc
     1861 cgacacctcg ccgtcgcctt gggcgtaatg cacgtcgccc acgatcagcc cgcaaccatc
     1921 tatgtagcag ggcagcagca gcgtggtgcc gaccaccatc tgcttgatgt ccatgttgcc
     1981 gccgttctcg cgcggcggga tggtgcgcag gcacttgtcc ttgtggctgc cgttggggcc
     2041 gcacaccgcc gccggcaggg ccccggtggg ctgcggcggc agcgcgatcc cgccggcctc
     2101 acccagctgc ttctcgcgcg caaaccaggc gtcggtctcg gggaagccgg gcatcgtgcc
     2161 cacggtgccc atgaagccgt tgaaggggat ggcgaccccg gggatctggt cggacaccgc
     2221 ctccatgcgg ttcagcttcc agttggcgat gaacggctcg gtgaacaggt cgcgcaggaa
     2281 gccgaagccc ggcacgatca gcgtgtagcc gtactcgtcg ggatcgacgt ccaccagcgt
     2341 gacagcgagc acgtcaccgc gcatcgcccc ttcgatatga accggcccgg tgatcggatg
     2401 caccaggttg aggtccaggg cggccatgtc cttcgccgtg gaattgaccg tgaggtcgga
     2461 gtcgagcgcg tctcgggttt cgaacacgat cagctggccc ggcctggccc gcgccgccgg
     2521 cttgatcgcc gggtgatagc ggttgatgca gttcggatcg tcggcgcaat gcgcgccggt
     2581 tttttttacg tgcacccagg actgggtctt gacgtcggtg gtgtcggcgc cggccggcac
     2641 agccagagac gcggctgcca acgcggcgca cagggcggcg aagcgcgctt tcatatctct
     2701 ctccctgaag ttaggaaaaa ccggaactgc ggtgggggga acgaaactcc ttcttattct
     2761 cgccggacgg gcgtcagttt ggccgcgccg ccctgtcgtt gtcaaaccac tgggccgttg
     2821 tcaaacggct gggtcgttgc caaaccgctg ggtcgttgcc gaaccgccgg gccgttgcca
     2881 aaccgccgcc gaccgtgcaa tcgcccgcct gtccgatcag gcaccgggca gcaggcgctc
     2941 cacggtccag tacaggccaa gcagcagcac gctggcggac accgcgtagg ggtaggcagt
     3001 gggaaacgcc gggaacggtc cccgcagcgc gcgctccacg ccgcgcatca ggcccagcgc
     3061 cgccaccacg atcacgatct ggcccacctc gacgccgacg ttgaaggccg ccagcgccgg
     3121 caccagcgca tcgcgcggga tcccgtagtc gcgcaacacc gaggcgaaac cgaagccgtg
     3181 gatcagcccg aaggcgaagg tgatccaggc gcgccggcgc atgtcgcgca cgaagaagtt
     3241 ttccgcggcc acgtagacga tggacagcgc gatcgcggtt tccaccagct gcgagggcag
     3301 ccgcaccacg tcgagcacgg ccagagtgag cgtgatcgag tgggcgacgg tgaacgcggt
     3361 gaccacgccc accagcggcc acacgcgccg tccccacagg atcaccgcca ccaggaaggc
     3421 gatgtggtcg tagccgatgg cgatgtgctc gatgcccgcg agcaggtaat gccacatgac
     3481 ctcgccgaca tgtgggcggg tgtcggacag ggccaccctc gggttgccgg tggacagcag
     3541 cgccatgcgc cgcacgtcgc cgccggcggt gaccatgtgg cgcgcggccg ggtcgacctc
     3601 ctggaacagc gtgacctcgt agatcagctg cccggtcatg ggcgggcagc tccactcgat
     3661 gtccaccagc acgtgctcgg ccttcgggcg ggcgccggcg aggcgcccat ggcaggccgc
     3721 cccggcggta ttggagagct tcgcccgctc cagtatgtag gcgccgagct gttgctcgac
     3781 ctcgcgcagc gcggcggggg cgacttgccc gtccgcgccc aacagcgcga cctcgagcgc
     3841 cacctgcacg tcgagcgcgt tcaactccag cagcacgtcg atccgaccgc cgtgcacctg
     3901 cgcgcgcgag ctggacagct gcgtggcgtg cggccaggcg ctcccggcgg cgagcaggca
     3961 ggcgctcgcc gtcaggaacg cggcgaaggt gcgcaggcgg cggaacatcc gatctggaaa
     4021 tccgggctgg agaccgcgaa gcccggcgtt cgaggaacgc gagggccgga ggccgcatcc
     4081 taccatcccc ctccgaaccc ctcccccggg aagagcaggc ggccggcgag ggccgtcacg
     4141 cccggcggaa cgggttgatg cggccgaagc gctcggccgc ccgctggcgc tgcggatgcg
     4201 cgatatggcg caggcgctcg gactcggggt cgagatacag cgcggcaccc agcgcgcgct
     4261 cggcatcgtc cgcatgcgtg gcgtagcgga cttcgaaagc gaatgcccag gacaccggcc
     4321 aggcgcgttg ctgccgccgc gcgaggtcga gcagcagctg tcgatagcgc tcgtccgcgc
     4381 tgagggccag cagccgctcg caggcttcga gcacctggta gccggcgggc accgcgctgt
     4441 cgagatgacc cggggcggcg agaaaagcgt cccacagcgc acccagcgca cgctcgtgct
     4501 cgcccctggc gccggcggcg taagccgccg ccagcgtggt gtagaaggcg gacccgggct
     4561 gcttgccggc cgcctggagc agcgcttgag cgtcgccgcc gcggccggtg ccgtacagag
     4621 ccagcaccag ccagggcagc gtctccggct cctggccgcc ctgttgcaac ggttccagct
     4681 cgccgaccac cttggcgtag tcgccgcgct tgaaggcggc gtagccgcgc accagggcgg
     4741 cgaacgcggg gtcttcgcgc tcccgggcga agctgctcag cgcatcgacc gcccagtccg
     4801 cccccggtcg gtccaccagc agcatgttga acagaaaccg ctccttgagc cgcgtctcgc
     4861 gcgcacgctc cccggacagg ctcttccagc gcttggcgaa ggccaccacc tcatccggct
     4921 cggtggcttc gagccggtgt ccgtcgaccg ccgccgacca cggcgcgaga gtctcgaagc
     4981 gcttggaagc ctcgtagaat gcgcgccagc cagcctcgcg ctcccccagc aggaaggcga
     5041 gcgtgccggc gcgcgccagc gcgtgctcgg cgccgtgctg ctcgtgcaag gcgagcgcca
     5101 gcgtgcgcac ctcggaccag tcgccgtcga gctgagccag ccgcatgcgc gcctccagtc
     5161 gcggcgaagg ctcgctgccg tcctccgccg cccgcgccgc cagctcgagc aacggccgcg
     5221 cgaactcgcc ctcgccggcc gccagcagca gcgcgcccga ctcggccgcc tggcgcaccg
     5281 ccccgcgtgc tccggcgccg ttgaacgggg gatagctgag caacgcacgc tgcgccatcg
     5341 ccggttcgcg cgcgcgcagg tatgccttgg cgagcagcgg gtacgcggcc cattgctcgg
     5401 gctgctcgcg gatgcgctcc agcaacagca cgaccgcggc ggcgaggtcg cgttcggctt
     5461 ccgcccgctg cagcaggaag cgctcgcgca ccgggctgcc gacgaaacgc gtgcgcgcca
     5521 gctgcatgag ctgggcgacg cgatccggct ggcgtgcgcg ctcgtatgct tggatcgcgc
     5581 gctgcaagac ggcgaaatcc tcctgcgcgt aggactgcat gcgtgcgtag tgcatcgccc
     5641 ccgccagggc ggacgcaccg gcctcgccct cggcggcggg gactgcgcgc caggggcgtg
     5701 ggggttcgtc cgcctgcggc ggctgcacag gctgcaggcg cgcgcgcagc tcgcgctcgg
     5761 tgtcgctttc gccgccttcc caggccagga cgtcggcctg cagccggcgg ctgcgctcgg
     5821 cgatgaggaa ggcgcgctcg ccgccgcccg cgccaagccg ctgcagcacc accgcgcgcg
     5881 ccgcctggat gccggcgtgg ccgccgaagc ccgaccccag cgcgcgcaga tcgtcgagga
     5941 gctgttgatc gctgtccggc gcggccgcca gcgcccacaa gcgcagtact accgcagccc
     6001 ggttcgccgc gtacagcgcc tcggggatgt cccacgcggc cacccgatcg aagccgcgct
     6061 ccgcgcgcca cgcgcccgcg ttcgcacgcc acagcgcggc gcgcccgcgc tcgaggtcgg
     6121 ccgccagcgc gaggccggcc ccgcgccccg gcaccttgat cccaactcgg gccgcgagag
     6181 ccgcgatccc gggcggctcg gcgtccgcac ccagcgcggc gagctgcgcg gcgaccactg
     6241 catgtgcgtc gcccgtcgca gcatcgcccg gcgacatcgg cacgtacagc agcgcggcgt
     6301 atcccggcca gctgtccagc agacgctggc ggcgctcgct gttggccctg tgcttgccat
     6361 agtgcgcacg cagtgcggcc agctccagat cgagcgtcag cgccgcggcg tcggccagct
     6421 cggctcggcg ccgttcggcc tccagcagat tgccgtcggc cagcgcgcgc accgcggccg
     6481 gctcgctgcc gccgaggccg tccagcagct gcagcgcgta cgggcgccgc tgcaggtgca
     6541 gcgccgcacg ggcgcgcagc acgcgtgcct gaccgtgcgt cgccggcagc accgacagcg
     6601 ccacgatgga acgctcccac agatgctcgc cgttcagctc gctggcgtga tgcgcggcgg
     6661 ccagcaactg cagcgcgcgt gcgcgcgcca gcggcgtcgc gccgtagccg gccaggtggc
     6721 tcgtcgtgcc gggtaacctc cccggctcat cggcctgcgc ctgctgcgcg tcctccccgg
     6781 tgagcgggat gccgagcgct tccgccgcct cgcgcgcgat gcgcgcgaac gccgcctccg
     6841 ggggcagttc gtcggacacc ggcagcggac cccacaccgc ctcgccgcca gcctcccagg
     6901 gagcctgcgc ctgggtcctg gtgtacagct gcagcgcaac gtggaacgcg ggccgttgct
     6961 cgtccatgcg cacctcgccc tgcaccaggt aacgcgcgtt cagcgcgttg gcgagcttgc
     7021 gcaccgcgcg ctgggcgcta ccgcgatcct gcgccccgag cgcccgcgcc accagggtgg
     7081 gatcggccac gcacaggccg ctgcgccgcg ccagctccgc ggcggtgagg cgtgtgatga
     7141 gcgagcgcgc agcccgatcc agcgacggcg cggcgccggc gaccggcacc accaggacct
     7201 cgcagctcca cccggcatgc gcttcggtga gcaggctgct ttcgcgctgc acccagccgg
     7261 atgtgcgcgc gcgctccggg gcgagcgcca tcgcggcgcg gatcggcgca tcgggcagca
     7321 tgactgcgac atagccgacg gcccccagga cgagcacggc gaccagcatg tgcaccgcga
     7381 tcggccgcac ggcgcgaaag gcgccgccgt cgccgcgcca ggctgcgagc agggtttcga
     7441 tcccggccag cagggcgatg ccggcgagca gcggcgccac gccgaccgcg aagaacagca
     7501 gcggcgcagc cgcctcggcc caccaggcgc cgctgcccac ggccagctcg gtgaacacgc
     7561 cggccacttc ggtggcgatc caggtcgcgc cggtgcccgc cagcgccagg cgcagcagcg
     7621 gcgaggtgtg caggttgacg tagtcggtga gccggtcgct cgcacgccac gcggccacgc
     7681 ccagcagcgc cccgcccacg gcgtgcaccg cgcagcgcag ccacaagtgc ccatgataat
     7741 cggtgagcag caggtaaccg gcgcccgcgg cgagcgcggc aaagccgagc acgctcaacg
     7801 gcaggcccca gaagaaaccg atgtggaatc gcatggtctg cggaaggact tccgcagcca
     7861 tgatcggcac gcacgccggg aaattgaggg acagtcgccc ggcggctacg cgcccgaaac
     7921 gccggcggct acgcctctag cacaccgggc agacgctcca gctgccatag cacgaacgca
     7981 taggtgaaag cgatctcgcg caggaagtcg tagcgtccgg aggccccgcc atgccccgcc
     8041 tccatgttga tcttgagcag cagcgggcgc gtgtcggtct tggtggcgcg cagcctggcc
     8101 acgtacttgg ccggctccca gaccatcacc tggctgtcgt tgagcgaggt ctgcaccagc
     8161 atcgccgggt aggccttggc ctcgatgttg tcgtaaggcg agtaggacag catgtactcg
     8221 aagtagcgcc ggtcgcgggg atcgccccac tcctcgtact cgccggtggt gagcggaagc
     8281 cgggggtcga gcatggtgtt gatcaggtcc aggaagggca cgtcggcgac caccgccttg
     8341 aacaggtcgg gacgggcgtt ggtcaccgcg cccatcagca gcccgcccgc gcttccgccc
     8401 tcgatcacca ggcggtcggg gcgggtgtag ccgcgcgcga tcaagtactc cgcggcggct
     8461 acgaagtcgg taaaggtgtt gcgcttgttg agcatgcgcc cgccgtcgtg ccagcgcttg
     8521 cccatctcgc cgccgccgcg cacgtgggcg gtggccacga cgacgccgcg gtcgagcagg
     8581 gacaggcgcg ccgagctgaa gcgcacgtcg tgggggaaac cgtaggcgcc gtagccggtg
     8641 agcagcatcg cctgcggccc ggacgcgcgc agctcgcgcc ggtagaccag agacaccggg
     8701 atgcgctcgc cgtcggcagc ggtggcatac gtgagctcgg aagcgtagcg cgccgcgtcg
     8761 tagccgccca gcaccgggcg ctgcttgagc agca
//
LOCUS       DASPGE010000118         3167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1321714, whole genome shotgun sequence.
ACCESSION   DASPGE010000118 DASPGE010000000
VERSION     DASPGE010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321714"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VD791_03740"
     CDS             complement(<1..781)
                     /locus_tag="VD791_03740"
                     /inference="COORDINATES: protein motif:HMM:NF021645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HXV07115.1"
                     /translation="MLRKLDMLYCAVSRYLCALLPLFAPAPAAADLLAYVRKPDPASA
                     WHAVGNRALGAGTVHTLRLVSQRWQGSAWQHDLRLYVPDQRGNHSTLLLFVTGGGGGD
                     ESAMRLAHSLRAPVGVLGEVPNQPLLDGKVEDDLIAESFLRYLATGDAEWPLLLPMTK
                     SVVRAMDALQAFAREALGREVQGFVLTGASKRGWASWLAAAADPRVRGVAPRVIDMLD
                     VAAQMPHQLASWGVYSEMLQPYTDPGLPSFIDTPAGRRLLAM"
     gene            842..1597
                     /locus_tag="VD791_03745"
     CDS             842..1597
                     /locus_tag="VD791_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HXV07116.1"
                     /translation="MFAHPGHLLVIGHLLVIALLIAAPAARADVYQCIGAEGKMLLTD
                     SACPPGYRTNLVVSDPQPQVVAAAPQPAAGATPPASASAAAMDRRVLEAEAEAELLRD
                     QLEIERLRSDLVRERLDAIEDHLDGLTDPYAVYGGLAVPLLVTPKPRFHGPHGTPDRF
                     HDKPGKLHDRRPCRDCRPRLVDGKPRVDGKPRVDGKPRVDGKARVDGKPRDVKPRVVP
                     RHARRDCGTFGCTPTITHAPWDFPRRGERRTQR"
     gene            1699..2313
                     /locus_tag="VD791_03750"
     CDS             1699..2313
                     /locus_tag="VD791_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07117.1"
                     /translation="MARPAINPGTVDWTGENPGMYLKETADGPFVTLISFFRVLLSPR
                     GRGHAAFILQDPHGEGRSAAKPNLCITDNEPLAEYLREGFVANFGAFRGLKGLAGVRM
                     VPGWDFMPAGDGKVAHVEWFRSAVGQVCLTWKGLGDVFLVEMPKDKSATGRHEMFSLF
                     VDAHAVDATINGRGVAGRAFPREFAGKKDSSTAFLAFSETWVKA"
     gene            2399..3013
                     /locus_tag="VD791_03755"
     CDS             2399..3013
                     /locus_tag="VD791_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07118.1"
                     /translation="MTLSAARLLVPAATALVLGGCASTSLVNQWKSPEFSGPPVRKVL
                     VVGVTQQASVRRVFEDEFAGALRAAGVEAVPSYTIIAEDGQAEQALLEKAVQDQGADG
                     VLVTRLVKSEQKTQVTPGFYHPAPAMGFYGWYSSAWVGYYEPPMVYQYDVVTAETSLY
                     SPPQSRLVWSGTTETFSPRDVKKETQGFAKVIIGALREHGIVAR"
BASE COUNT          487 a         1087 c         1137 g          456 t
ORIGIN      
        1 ccatcgccag caggcgccgc cctgcgggcg tgtcgatgaa gctcggcagg ccggggtcgg
       61 tgtagggctg cagcatctcg ctgtagacgc cccaggaggc gagctgatgc ggcatctgcg
      121 ccgccacgtc gagcatgtcg atgacgcgcg gcgccacgcc gcgcacccgc gggtcggcgg
      181 cggccgccag ccagctcgcc cagccgcgct tggaggcgcc ggtcaacacg aacccctgca
      241 cctcccggcc gagcgcctcg cgcgcgaacg cctgcagcgc gtccatagcg cgcacgacgc
      301 tcttggtcat cggcaacagc agcggccact ccgcgtcgcc ggtggcgaga tagcgcagga
      361 aactctcggc gatcaggtcg tcctcgacct tcccgtcgag caacggctgg ttcggcacct
      421 cgccgagcac cccgaccggc gcccgcagcg agtgcgccag ccgcatcgcg ctctcatcgc
      481 cgccgccgcc gccggtgacg aacagcagta aggttgagtg gtttccacgc tggtccggta
      541 cgtacaggcg caggtcgtgc tgccaggccg acccttgcca gcgctgcgat accaggcgca
      601 gcgtgtgcac ggtcccggcg ccgagcgcgc ggttgccgac ggcgtgccag gcggacgccg
      661 ggtcgggctt gcgcacatag gcgagcaggt cggcggcggc cggcgcgggg gcgaacagcg
      721 gcagcagcgc gcacaggtag cgcgagacgg cgcagtacag catatcgagc ttgcggagca
      781 tgtcgtcagc gaactgccgg cgcaggtgtt agtctgaact gtaccaagcc tcgccacggc
      841 catgtttgcg caccccgggc atctcctagt catcggccat cttctggtca tcgctctgct
      901 gatcgcggcg cccgcggcgc gcgctgacgt ttaccaatgc atcggcgccg agggcaagat
      961 gctgctcacc gacagtgcct gcccgcccgg gtaccgcacc aacctggtgg tgagcgatcc
     1021 gcaaccgcaa gtcgttgctg cggcgccgca gccggcggcc ggcgcaacgc cccccgccag
     1081 cgcttcggcc gcagccatgg accggcgcgt cctagaggcc gaagccgaag ccgaactgct
     1141 gcgggaccag ctggagatcg agcggctgcg ctccgatctg gtgcgcgagc ggctcgatgc
     1201 gatcgaggac cacctcgatg ggctgaccga tccctacgcg gtgtacggtg gcctggcggt
     1261 gccgctgctg gtgacgccca aaccgcggtt ccacgggccg catggcacgc cagatcgttt
     1321 tcatgacaag ccgggcaagc tgcacgatcg ccgtccctgc cgggactgcc ggccgcgcct
     1381 tgtcgatggg aagccccggg tcgatgggaa gccccgggtc gatgggaagc cccgggtcga
     1441 tgggaaggcc cgggtcgatg gaaagccccg ggacgtcaag ccccgggtgg tgccacggca
     1501 cgccaggcgc gactgcggga cgttcggctg cactcccacc atcactcacg ccccctggga
     1561 cttcccgcgc cgcggcgaac gccgaacaca gcgctagccc gcaggcaccg ctctccccgc
     1621 tgcgggagag gggccggggt gagggggtgt ttcaacaact gctagactgc ggccgccgaa
     1681 caacagggag gagaaaccat ggcacgtccc gcgatcaacc ccggcaccgt cgactggacc
     1741 ggcgagaacc ccggcatgta cctgaaggag acggcggacg gccccttcgt cacgctgatc
     1801 agcttctttc gcgtgctgtt gagcccgcgc ggccgcggcc acgcggcctt catcctgcag
     1861 gacccgcacg gcgagggcag gagcgcggcc aagcccaacc tgtgcatcac ggacaacgag
     1921 ccgctggccg agtacctgcg cgagggcttc gtcgccaact tcggcgcctt ccggggcctg
     1981 aaagggctgg ccggtgtgcg catggtgccg ggctgggact tcatgcccgc gggcgacggc
     2041 aaggtcgcgc acgtggagtg gttccgcagc gccgtcggcc aggtgtgcct gacctggaag
     2101 gggctgggcg acgtgttcct ggtggagatg ccgaaggaca agtcggccac cggccggcac
     2161 gagatgttct cgctgttcgt ggatgcgcac gcggtggacg caaccatcaa cgggcgcggc
     2221 gtggcgggcc gggcgttccc ccgcgagttc gcgggcaaga aggacagcag caccgccttc
     2281 ctggcctttt cggagacctg ggtgaaggcg tgagggcgca gcctcgcgcc gcggcgccgt
     2341 acctggggcg ctgcggcaca atgcgggcgt ccccgccggg tttcctggga gcagatcgat
     2401 gaccctgtcc gccgcgcgcc tcctggtgcc ggctgcaacc gcgctggtcc tgggcggctg
     2461 cgcttccacc agcctggtca accagtggaa gagcccggag ttctcgggtc cgcccgtgcg
     2521 caaggtgctg gtggtgggcg tcacccagca ggcgagcgtg cggcgcgtgt tcgaggacga
     2581 gttcgccggt gcgctgcggg cggccggcgt ggaagcggtg ccgagctaca cgatcatcgc
     2641 cgaggatggc caggccgagc aggcgctgct ggagaaggcg gtacaagacc aaggcgccga
     2701 cggcgtgctg gtcacgcgcc tggtgaagag cgagcagaag acgcaggtca cgcccggttt
     2761 ttatcatccg gcgcccgcga tgggcttcta cggctggtac tcctccgcct gggtcggcta
     2821 ctacgagccg ccgatggtgt accagtacga cgtggtgacc gccgagacca gcctgtacag
     2881 cccgccgcag tcgcggctgg tgtggtcggg tacgaccgag acgttctcgc cccgggacgt
     2941 gaagaaggaa acgcagggct tcgccaaggt gatcatcggc gcgctgcgcg agcacggcat
     3001 cgttgcgcgc tgaggctgct gcgctgccgc agcagctccg cgcgtagttt ctccttgaca
     3061 gcggcgcgcg ccgcgctaaa gattgcggca cggcggcgcc cggtggcgcg cttcaggcgt
     3121 tgcagcgcgg ccggtttcgt caccgagaag tattcctaca ccaatcg
//
LOCUS       DASPGE010000119         3777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_385396, whole genome shotgun sequence.
ACCESSION   DASPGE010000119 DASPGE010000000
VERSION     DASPGE010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385396"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..524)
                     /locus_tag="VD791_03760"
     CDS             complement(99..524)
                     /locus_tag="VD791_03760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HXV07119.1"
                     /translation="MTQIDFYTHVESRYQTLCVLAGKALARGLRMMILTPHSEATEQV
                     DRLLWSQPAIGFLPHCRAHHKLAGVTPIILDHVAEPVVHEQLLVNLCDQPPPHFSRFE
                     RLIEIVGLDDAERAQARARFRFYRERGYEIRTHDLHTAA"
     gene            complement(521..2053)
                     /locus_tag="VD791_03765"
     CDS             complement(521..2053)
                     /locus_tag="VD791_03765"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HXV07120.1"
                     /translation="MEFSTNAAAPEKCRSDCIVVGVFDGRRLTAAAQAVDRACKGRLA
                     AVLARGDLAGRLGSTLLLHDVPGTAAQRVLLVGLGKQEEFGPKAYREAVQAAVRALSD
                     GGTSDAVLYLGTASISASDDKAKQRDGAWAAMTAVVVAADAGYRFDRLKSKKEDRKGL
                     ERLAIGSADAAHKVAVARGAALAEGMALAKDLGNLPSNICTPSYLARQAQDLARRHKL
                     ACEVLGEKDMEKLGMGALLAVTRGTREPAKLIVLRYQGAARKDKPVALVGKGITFDTG
                     GISLKPSAEMDEMKYDMSGAGSVLGTLEAAARMRLPVNVVGVIPTCENMPGGSAIKPG
                     DIVASMSGQTIEILNTDAEGRLILCDALTYCERFEPDAVVDIATLTGACVVALGHVAT
                     GLYSNDDGLAQALLDAGERAWDRAWRMPLWDDYQEQLKSPFADMGNIGGRPAGSVTAA
                     CFLSRFAGKFKWAHLDIAGTAWRSGKDKGSTARPVPLLTTFLMDRAGGAGRPRGRAAR
                     KT"
     gene            2211..3290
                     /gene="lptF"
                     /locus_tag="VD791_03770"
     CDS             2211..3290
                     /gene="lptF"
                     /locus_tag="VD791_03770"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HXV07121.1"
                     /translation="MIFLRSTLRELTTAGVAVFAILLVITFTSQLIRLLGSAARGRIP
                     ADAVMTLMGFAALGYLSVLLSATVFLAVLLTLTRAYRDSEMVVWQTSGLGLISWFRPV
                     IVFAAPVVLTVAVLSTTLTPWAVGKVEQYRHQLANRDDVSAVSPGVFKESRNAERVFF
                     VENLSSDLTQVTNIFVHELRKQRQGVVVARRGYLETTDEGDRYLVMADGRHYAGTPGQ
                     ADYRVETFGRYALRIEQSESKSFLPSHKSRPTRELLADPSPANMGELGFRFGLPVATV
                     ILALLAIPMSAVNPRAGRAVNLIAAVFVFMIYSNVVSLSQAWIAQRKLPPEIGVWIVH
                     AVMVGVLAVLLARRMGRLRIRLRRR"
     gene            3287..>3777
                     /locus_tag="VD791_03775"
     CDS             3287..>3777
                     /locus_tag="VD791_03775"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HXV07122.1"
                     /translation="MRILRRYLATQIVASVVLVLTALLMLFSFFDLINELQDIGQGAY
                     SLAAALTYVGLSVPGRLYELFPAAVLIGTMFALAQLVLNAEYAVMRTSGVSITGLALA
                     IAQVGLAFAALAFVTGEFLTPLSEEAAQRLRLRQTSSVIASTFRSGLWVKDSDSFVNV
                     GRI"
BASE COUNT          507 a         1318 c         1300 g          652 t
ORIGIN      
        1 ccccggcagt cttgttccat gcggcggggc gcgcccctgg tgtcgactga tgagctgatc
       61 cagcttgtcg aacagatcgt cgtcgtcgtc ggtcggggtc atgctgcggt atgcaggtcg
      121 tgcgtgcgga tctcgtaacc gcgctcgcgg tagaagcgaa agcgcgcgcg cgcctgtgcg
      181 cgctccgcat cgtccaggcc gacgatctcg atcagccgct cgaagcggct gaaatgcggc
      241 ggcggttgat cgcacaggtt caccaggagc tgctcgtgca ccaccggctc ggcgacatga
      301 tccagaatga tgggcgtcac gccggcgagc ttgtggtgcg cgcggcagtg cggcaggaag
      361 ccgatggccg gctggctcca cagaagcctg tccacctgct cggtcgcctc gctgtggggg
      421 gtcaggatca tcatgcgtag cccccgcgcc agcgccttcc ccgcgagcac gcacagcgtc
      481 tggtagcggc tctcgacgtg ggtgtagaag tcgatctgcg tcatgtcttg cgcgccgctc
      541 ggccgcgcgg ccggccggcg ccgccggccc ggtccatcag gaacgtggtg agcagcggca
      601 ccggccgcgc ggtggagccc ttgtccttgc cggagcgcca ggcggtgccg gcgatgtcga
      661 ggtgggccca cttgaacttg ccggcgaagc gcgacaggaa gcaggccgcc gtgacgctgc
      721 cggccggccg gccgccgatg ttgcccatgt ccgcgaacgg gctcttgagc tgctcctggt
      781 agtcgtccca cagcggcatg cgccaggcac ggtcccaggc gcgctcgccg gcgtcgagca
      841 gcgcctgcgc cagcccatcg tcgttgctgt acagaccggt ggccacgtgg cccagcgcga
      901 cgacgcaggc gcccgtgagc gtggcgatgt ccaccacggc gtcgggctcg aagcgctcgc
      961 agtaggtcag cgcatcgcac aggatcaaac gcccctccgc atccgtgttg aggatctcga
     1021 tggtctgccc ggacatgctg gcgacgatgt cacccggctt gatggcactg cccccgggca
     1081 tgttctcgca ggtcgggatc acgccgacca cgttcaccgg caggcgcatg cgcgcggccg
     1141 cttcgagcgt gcccagcacg ctgcccgcgc ccgacatgtc gtacttcatc tcgtccatct
     1201 cggccgatgg cttgagcgag atgccgccgg tgtcgaaggt gatgcccttg cccaccaggg
     1261 ccaccggctt gtccttgcgc gccgcgccct ggtagcgcag cacgatcagc ttggccggct
     1321 cgcgcgtacc gcgggtgacg gcgagcagcg cgcccatgcc gagcttctcc atgtccttct
     1381 cgcccagcac ctcgcaggcg agcttgtggc gccgggcgag gtcctgcgcc tgcctggcga
     1441 gataggacgg ggtgcagatg ttgctgggga gattgccgag gtccttggcg agcgccatgc
     1501 cctcggccag cgcagccccg cgcgcgaccg ccaccttgtg cgcggcgtcg gcggacccga
     1561 tggccagccg ctccagccct ttgcggtcct ctttcttgct tttcaggcgg tcgaaacggt
     1621 agccggcatc cgccgccacc accacggcgg tcatcgccgc ccaggcgccg tcgcgctgct
     1681 tcgccttgtc gtccgaggcg ctgatcgagg ccgtgccgag gtacagcacg gcgtcgctcg
     1741 tgccgccgtc gctgagcgcc ctcaccgcgg cctgcaccgc ctcgcgatag gccttggggc
     1801 cgaactcctc ctgcttgccc agcccgacca gcaggacgcg ttgcgccgcg gttcccggga
     1861 cgtcgtgcag cagcagcgtg gagccgaggc gaccggccag atctccgcgc gcgaggaccg
     1921 cggcgaggcg ccctttgcag gcgcgatcga cggcctgcgc cgcggcggtc agccgccggc
     1981 catcgaacac accgaccacg atgcagtcgc tccggcactt ttccggtgcc gcggcgtttg
     2041 tgctaaattc cacctggtgc tcctcgtttc gttcgattat tgaatcgctt agaattgcgc
     2101 gaattattgc ttcccgcacg ccttgaagtc aaagcatggc ggggagtcga tgcgtgccgg
     2161 cgccgcccgg cgcagcgtgc aggggcgccg cgctgcggga aggccagggc atgatcttcc
     2221 tgcgtagcac gctgcgcgag ctcaccacgg cgggcgtggc cgtgttcgcg atcctgctgg
     2281 tgatcacctt cacctcgcag ctgatccgcc tgctgggctc cgccgcccgc ggccgcattc
     2341 ccgccgacgc ggtgatgacg ctgatgggct tcgcggccct gggttacctg tccgtgctgc
     2401 tgtcggccac cgtgttcctg gccgtgctgc tgacgctcac gcgcgcctac cgcgattcgg
     2461 aaatggtggt gtggcagacc tccgggctgg ggctcatctc ctggttcagg ccggtcatcg
     2521 tgttcgccgc cccggtggtg ctgaccgtgg ccgtgctcag caccaccctc acgccgtggg
     2581 cggtgggcaa ggtcgagcag taccggcatc agctggcgaa ccgcgacgac gtctcggcgg
     2641 tgtcgccggg cgtgttcaag gaatccagga acgccgagcg cgtgttcttc gtcgagaacc
     2701 tgtccagcga cctcacccag gtcaccaaca tcttcgtgca cgagctgcgc aagcagcgcc
     2761 agggggtggt ggtcgcccgc cgcggctacc tggagacgac cgacgagggc gaccgctacc
     2821 tggtgatggc cgacggccgg cactacgcgg gcacgccggg gcaggccgac taccgggtgg
     2881 agaccttcgg gcgctatgcg ctgcgcatcg agcagagcga gagcaagtct ttcctcccgt
     2941 cgcacaagtc gcgccccacc cgagagctgc tggccgatcc ctcgccggcg aatatgggcg
     3001 agctcggctt ccggttcggg ctgccggtgg ccactgtgat cctcgccctg ctggcgattc
     3061 cgatgagcgc ggtcaacccg cgcgcggggc gcgcggtgaa cctgatcgcg gccgtgttcg
     3121 tgttcatgat ctacagcaac gtggtgagcc tgtcccaggc ctggatcgcg cagcgcaaac
     3181 tgccgccgga gatcggcgtc tggatcgtgc acgcggtgat ggtgggcgtg ctcgccgtgc
     3241 tgctcgccag gcgcatgggc aggctgcgca tccggctgcg acgccgatga ggatcctgcg
     3301 ccgctatctc gccacgcaga tcgtggccag cgtcgtgctg gtgctgaccg cgctgctcat
     3361 gctgttcagc ttcttcgatc tgatcaacga gctgcaggac atcggccagg gcgcctacag
     3421 cctggccgcg gcgctcacct acgtgggctt gagcgtaccc ggtcggctgt acgagctgtt
     3481 cccggccgcg gtgctgatcg gcaccatgtt cgcgctggct cagctcgtgc tcaacgccga
     3541 gtacgcggtg atgcgcacct ccggggtgtc gatcaccggc ctggcgctgg ccatcgcgca
     3601 ggtcggcctg gccttcgccg cgctggcgtt cgtcaccggc gagttcctca cgccgctgtc
     3661 ggaggaggcg gcgcagcggc tgcgcctgcg ccagaccagc agcgtgatcg cctcgacctt
     3721 ccgctccggc ctgtgggtca aggacagcga cagcttcgtc aacgtcggcc gcatcat
//
LOCUS       DASPGE010000120         8496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1557068, whole genome shotgun sequence.
ACCESSION   DASPGE010000120 DASPGE010000000
VERSION     DASPGE010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557068"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1890
                     /gene="uvrA"
                     /locus_tag="VD791_03780"
     CDS             <1..1890
                     /gene="uvrA"
                     /locus_tag="VD791_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXV07123.1"
                     /translation="HYGFDLDTPFEDLPARVREILLQGSGREKIRFRYAAERGAQHTR
                     EHVFEGIVPNLERRYKETDSAVVREELAKYLNTAPCPECEGTRLRREARFVFVGGHTI
                     YGLSRLPLARALEALRGLQLAGAKLAVAAKIVREIVERLRFLNDVGLDYLTLERSAET
                     LSGGEAQRIRLASQIGSGLTGVMYVLDEPSIGLHQRDNDRLLTTLKRLRDLGNSVIVV
                     EHDRDAIEAADFVVDMGPGPGEHGGRVVATGSPRDIARHPDSLTGQYLSGRRGVAMPL
                     TRHTPQPDRWLRVSGARGNNLKSIDFNLPAGLFTCVTGVSGSGKSTLVNDTLYNAAAR
                     HLYGAAQEPAPCDEIAGLELFDKAVNVDQSPIGRTPRSNPATYTGLFTPIRDLFAGVP
                     LARERGYGPGRFSFNVKGGRCEACQGDGVLKVEMHFLPDVYVTCDVCHGKRYNRETLE
                     VKYKGRSIFEVLQLTVEQAHEVFGPVPAIARKLQTLLDVGLGYITLGQSAITLSGGEA
                     QRVKLALELSKRDTGRTLYILDEPTTGLHFHDIAMLLRVLHQLRDHGNTVVVIEHNLD
                     VIKTADWIVDLGPEGGDGGGHIVAQGTPQQVAQHPDSHTGRYLRQALQQPAAATPRGR
                     STLPL"
     gene            2161..2550
                     /locus_tag="VD791_03785"
     CDS             2161..2550
                     /locus_tag="VD791_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HXV07124.1"
                     /translation="MSRSSRASNPSAAELDRAALDAIRALDEGGAAGLLQQVVQLYLE
                     SVPPLIAELRRAGAAGDASAVRNAAHSLKSSSANLGAARLAEMCRAVEHAARAGRLGG
                     DLPSVDEIEGEFRALRPALEREAGLAL"
     gene            2547..4700
                     /locus_tag="VD791_03790"
     CDS             2547..4700
                     /locus_tag="VD791_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXV07125.1"
                     /translation="MSTPAARVSAALIVDDDPTMRLLIRQTLERVGFACEEAENGNQA
                     LQRFVAAQPDIVLLDVMMPELDGYETCARLRAMPGGATVPVVMLTGLDDDESIDRAYQ
                     VGATDFISKPITWGVLGYRVRYLLRASHALRDVAKHQASLETAQRIAHLGGWEWNLQR
                     NEVYWSPETFRILGLAPDAVTPSVDRFLARVHPQDRAVAEQAISDLTRTGEFSRRLVR
                     LVRPDGSVRHVQLQGLGTFDARGAVTQLAGTIQDVTELKEAEQRIRHLAYYDGATGLP
                     NRQFFTERLAQTLALAKRHRGQLGVLALDLDQFKRINDTLGHTAGNELLVAVARRLAD
                     AVREADALGRPDGESAVPMEASDLIARLDGDEFCLLITDLAQYHDAAKVARRLLQELR
                     KPFRIGNREMFVTASIGLALYPLDGEDADSLLKNAGAAMHFAKEQGRDNYQFYSRAMN
                     ATALEKLSMESQLRKALERGELLLNYQPKIRVSNGQVVGLEALIRWRHPELGLVRPNQ
                     FIPVAEETGLIIRIGDWVLRTACLQNQAWRNAGYPPVHVAVNIASPHFRQGGLVASVA
                     AALKESALDPGLLEVELTESMLMHSVDNTLDTLSRLKDMGVRLAIDDFGTGYSSLSYL
                     KRFPLDTLKIDRSFVKDLPHDAEDAAITRAIIAMAHSLKLAVVAEGVENAEQLAFLQQ
                     HGCEVVQGYLFSQPLAAEDFGALLAAQREQSAAAG"
     gene            4823..5578
                     /locus_tag="VD791_03795"
     CDS             4823..5578
                     /locus_tag="VD791_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07126.1"
                     /translation="MPEPIPFPDGGYAYLQGGFPYSQGVRALDGYAIERARFAHPVPL
                     MQGFAAIEAQLARLGRPRTALCAAELRSPKPFTMDGFAEFNRGYVAVLERWGLFRDRR
                     NPVARSNVAPQIDPPAEPCFYAFCYTVPAAETAPGFVVAGSGEWPEGGRFPDDIVARG
                     DVSPAGVAAKARCVLDAMERRMAGLGVSWRNVTASQVYTVHDIHAVLGKEILPRAGNG
                     GGLTWHYCRPPIEELEYEMDVRGATVERVIPAP"
     gene            complement(5652..6635)
                     /locus_tag="VD791_03800"
     CDS             complement(5652..6635)
                     /locus_tag="VD791_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV07127.1"
                     /translation="MIGWLPWAGLAALALAPLSGIGNYPLHLAIMCLLWGFVYTGWAL
                     MGRLGMVSLGHGAFLGIGAYGVVMGWNHFGLSPWLGMPIALALTAVVAFLIGWPCFRF
                     KIVGHYFALVTLALSEVVRMIIVALRDQTGGSLGATPNTALAEQAFSLGAMQFSSKLV
                     WFYIMLVVWLAGLWVWTRVDRSMARLALEAIAHEEEAAASVGIHVTRLKLGVTLVSAL
                     MTCVGGVLYAQYQLYVNPETVSGIGISLQMVFGAIAGGMYVRLGPTVGAVFTLLLAEV
                     LRLALGHDIHGLDTTLYGLLLVLFIIFMPKGILGGAIEAWRRARRPEPAPA"
     gene            complement(6632..7501)
                     /locus_tag="VD791_03805"
     CDS             complement(6632..7501)
                     /locus_tag="VD791_03805"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV07128.1"
                     /translation="MDIFDIFLLEAVVNGVLLGGILALLALGLNLIFGVIDVVWICYA
                     ELIMIGMYAVYYLHVVYGWPLWLAFAAGVALVSVLGAALHYYVIRPVLAAEPINQLLV
                     TGGVLFLLQGVATLAFGVEFKNIGVRLPTLEVGEMFVSFARLIAFLAALAGMIGVYLF
                     LKRSYLGTAIRAISQDREIMPLMGVNPNRIFLITSALGGGLAGLAACLLVLQYDIHPA
                     IGLSFGPIAFLVCVLGGLGNMIGGFIAAFIFAQFISVGGYFFHLEWGYVIAFLFFIAV
                     MFWKPRGLFGGKS"
     gene            complement(7505..8209)
                     /locus_tag="VD791_03810"
     CDS             complement(7505..8209)
                     /locus_tag="VD791_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV07129.1"
                     /translation="MLSLQGVSAGYGSFQALFDIGLEVKAGETVAVIGPNGAGKTTLL
                     RVISRLIEPRAGEMTMEGKRLNAVPAHEVIGHGIAHVPENRRLFPRLSVEDNLRMGAF
                     TPAARARFDERLAAVYRHFPRMQERRRQPAGTLSGGEQQMCAIGRALMSGPKLLLLDE
                     PSAGLAPVVVQATFEVVRQMRAEGYTVLIVEQNIRQVLKVADRAYLLETGRIKLQGSS
                     AELLASEAIRKAYLGL"
     gene            complement(8226..>8496)
                     /locus_tag="VD791_03815"
     CDS             complement(8226..>8496)
                     /locus_tag="VD791_03815"
                     /inference="COORDINATES: protein motif:HMM:NF023816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV07130.1"
                     /translation="SLGGLDEKEMDQAAGLLARIRDERAITIIWVEHIMGVLMRVVDR
                     CVVLDHGELIAQGKPEEVAHDPRVIEVYLGSDAQDVQATVAARRG"
BASE COUNT         1441 a         3016 c         2862 g         1177 t
ORIGIN      
        1 cactacggct tcgatctcga cacgccgttc gaagacctgc ccgcgcgcgt ccgggagatc
       61 ctgctgcagg gctcggggcg cgagaagatc aggttccggt acgcggccga gcgcggcgcg
      121 cagcataccc gcgagcacgt cttcgagggc atcgtgccca acctcgagcg gcgctacaag
      181 gaaaccgact cggcggtggt gcgcgaggag ctggccaagt atctgaacac cgcgccctgc
      241 ccggagtgcg agggcacccg gttgcggcgc gaggcgcgct tcgtgttcgt cggcgggcac
      301 acgatctacg gcctgtcgcg cctgccgctc gcgcgggcgc tggaggcgct gcgcgggttg
      361 cagctggcgg gcgcgaagct ggcggtagcc gcgaagatcg tgcgcgagat cgtcgagcgg
      421 ctgcggtttc tcaacgacgt cggcctggac tatctgacgc tcgagcgctc ggcggagacg
      481 ctctcgggcg gcgaggccca gcgcatccgg ctggcgagcc agattggctc gggcctgacc
      541 ggcgtgatgt acgtgctgga cgagccgtcc atcggcctgc accagcgcga caacgatcgt
      601 ctgctgacca cgctcaagcg gctgcgcgat ctgggcaact cggtgatcgt ggtggagcac
      661 gatcgggacg ccatcgaagc ggcggacttc gtggtggaca tggggccggg gccgggcgag
      721 cacggcgggc gcgtggtcgc caccggctcg ccgcgcgaca tcgcacgcca ccccgactcg
      781 ctcaccggcc agtacctgtc gggccggcgc ggcgtcgcca tgccgctcac gcgccacacg
      841 ccgcagccgg atcgatggct gcgcgtgagc ggtgcgcgcg gcaacaatct caagagcatc
      901 gacttcaacc tgccggcggg gctgttcacc tgcgtcaccg gcgtgtcggg ctcgggcaaa
      961 tcgacgctgg tcaacgacac gctgtacaac gccgccgcgc gccacctgta cggggccgcg
     1021 caggaaccgg ccccgtgcga cgagatcgcc ggcctggagc tgttcgacaa ggcggtcaat
     1081 gtggaccaga gcccgatcgg ccgcacgccg cgctccaatc ccgcgaccta taccggcctg
     1141 ttcaccccga tccgcgatct gttcgccggc gtaccgctgg cgcgcgagcg cggctacggg
     1201 ccggggcgct tctcgttcaa cgtcaagggc gggcgctgcg aggcctgtca gggcgacggc
     1261 gtgctgaagg tggagatgca cttcctgccc gacgtgtacg tgacctgcga cgtgtgccac
     1321 ggcaagcgct acaaccgcga gacgctcgaa gtgaagtaca agggcaggag catcttcgag
     1381 gtgctgcagt tgacggtgga gcaggcccat gaggttttcg gcccggtgcc cgccatcgca
     1441 cgcaaactgc agacgctgct cgacgtgggg ctgggctaca tcacgctggg gcagagcgcg
     1501 atcacgctct ccggcggcga agcgcagcgc gtcaagctcg cgctggagct gtccaagcgc
     1561 gacaccggcc gcaccctgta catcctggac gagcccacca ccggcctgca cttccacgac
     1621 atcgcgatgc tgctgcgcgt gctgcaccag ctgcgcgacc acggcaatac ggtggtggtg
     1681 atcgagcaca acctggatgt aatcaagacc gccgactgga tcgtcgacct cgggcccgag
     1741 ggcggcgacg gcggcgggca catcgtggcc cagggtacgc cccagcaggt ggcgcagcat
     1801 ccggacagcc acaccggccg ctatctgcgc caggcgctgc agcaacccgc cgcggccacg
     1861 ccgcgcggcc gctcgacgct gccgctgtaa cgcaccgctc cccgcctctc gcgaggcggg
     1921 gtggcgccgc aggcgccggg gcggtgtggg gtggaagcat tgcagtacca ctgcgtcacg
     1981 cttcgcgtgc caccccgccg agtgcgccct caaggcgcga ctcgaaaacc cgctccccgt
     2041 ctataaagac ccatagtctc gatccgataa aacttcgggg cggtccagtc accgcgccgc
     2101 ccgtcggcat cctgcaatcg aatcgtcaaa cggccgctcg tcggctagca ctcaggccca
     2161 atgagccgtt cctcgcgcgc cagcaacccc tccgccgcag agctggatcg cgcagcgctg
     2221 gacgccatcc gggcgctgga cgagggcggc gcggccgggc tgctgcagca ggtcgtgcag
     2281 ctgtatctgg aatcggtccc gccgctgatc gccgagctgc gccgcgccgg cgccgccggg
     2341 gacgcctccg cggtgcgcaa cgccgcgcac tcgctcaaat cgagcagcgc caatctgggc
     2401 gccgcacggt tggcagaaat gtgcagagcc gtcgagcacg ccgcccgcgc aggcaggctc
     2461 ggtggcgacc tgcccagcgt cgacgagatc gaaggcgagt tccgggcgtt gcgcccggcc
     2521 ctggaacggg aagccggcct cgcgctatga gcacgcccgc cgcccgcgtc tcggccgccc
     2581 tgatcgtcga cgacgatccg accatgcgcc tgctcatccg gcagacgctg gagcgggtgg
     2641 gattcgcctg cgaggaagcc gaaaacggaa atcaggcgct gcagcgcttc gtcgcggcgc
     2701 agccggacat cgtgctgctg gacgtgatga tgccggagct ggacggctac gagacctgcg
     2761 cgcggttgcg cgccatgccg gggggcgcga cggtgccggt ggtcatgctg accggtctcg
     2821 acgacgacga atcgatcgac cgcgcgtacc aggtcggcgc taccgacttc atcagcaagc
     2881 cgatcacctg gggggtgctg ggctaccggg tgcgttacct gctgcgcgcc agccatgcgc
     2941 tgcgcgacgt ggccaagcac caggcgagcc tggaaaccgc gcagcgcatc gcccacctcg
     3001 gcggctggga gtggaacctg cagcgtaacg aggtgtactg gtcgcccgaa accttccgca
     3061 tcctggggct cgcgcccgac gcggtgacgc ccagcgtgga tcgattcctg gcgcgggtgc
     3121 acccgcagga ccgggccgtg gccgagcagg cgatctccga cctcacgcgc accggcgagt
     3181 tctccagacg gctggtgcgc ctggtgcgcc ccgacggcag cgtgcggcac gtgcagctgc
     3241 aggggctggg gaccttcgac gcgcgcggcg cggtcacgca gctggccggc accatccagg
     3301 acgtcaccga gctgaaggag gccgagcagc gcatccgcca cctcgcctac tacgacggtg
     3361 ccacggggct acccaaccgg cagttcttca ccgagcggct ggcgcagacg ctggcgctcg
     3421 ccaagcgcca tcgcgggcag ctcggcgtgc tggcgctcga tctcgaccag ttcaagcgca
     3481 tcaacgacac gctcgggcac acggccggca acgagctgct ggtcgcagtg gcgcgacgtc
     3541 tggcggacgc ggtgcgcgag gccgacgcgc tgggacgccc cgacggcgag agcgcggtcc
     3601 cgatggaggc gagcgatctc atcgcgcgcc tggacggcga cgagttctgc ctgctgatca
     3661 ccgatctcgc ccagtatcac gacgcggcca aggtcgcgcg ccggctgctg caagagctgc
     3721 gcaagccgtt ccgcatcggc aaccgggaga tgttcgtcac cgccagcatc ggcctcgcgc
     3781 tgtatccgct ggacggcgag gacgccgaca gcctgctcaa gaacgccggc gcggcgatgc
     3841 acttcgccaa ggagcagggc cgcgacaact accagttcta cagccgcgcc atgaacgcca
     3901 ccgcgctgga gaagctgtcg atggaatcgc agctgcgcaa ggcgctcgag cgcggcgagt
     3961 tgctgctcaa ctaccagccc aagatccgcg tctccaacgg ccaggtcgtg ggcctggaag
     4021 cgctgatccg ctggcgtcac cccgagctgg ggttggtgcg gcccaatcaa ttcatcccgg
     4081 tggcggagga aaccggcctg atcattcgca tcggcgactg ggtgctgcgc accgcctgcc
     4141 tgcagaatca ggcctggcgc aacgccggct atccgccggt gcacgtcgcc gtcaacatcg
     4201 ccagcccgca cttccgccaa ggcgggctgg tcgcatcggt ggccgccgcg ctcaaggaaa
     4261 gcgcgctcga ccccgggctg ctcgaagtgg agctcaccga gagcatgctg atgcacagcg
     4321 tggacaatac gctggatacg ctgtcccggc tcaaggacat gggggtgcgg ctggcgatcg
     4381 acgacttcgg caccggctac tcgtcgctgt cctacctcaa gcgcttcccg ctcgacacac
     4441 tcaagatcga ccgctccttc gtcaaagacc tgccgcacga cgcggaagac gcagccatca
     4501 cccgggccat catcgccatg gcgcacagcc tgaagctggc ggtggtggcc gagggcgtcg
     4561 aaaacgcgga gcagctcgcc ttcctgcagc agcacggctg cgaagtggtg cagggctacc
     4621 tgttcagcca gccgctcgcc gccgaggact tcggcgcgct gctggccgca cagcgggagc
     4681 agtcggcggc cgccggctag gcgagaagcc tgtcacgtcc ccggattccg ctgcgctgca
     4741 tccgggctac gaaccagcgc cgggcgagcg cgcgccccga gagcgcgcta cagttgccgc
     4801 cgtcgacctg cacggaggcc ccatgcccga acccattccc ttccccgacg gcggttacgc
     4861 gtacctccag ggcggatttc cctattcgca gggcgtgcgg gcgctggacg gttacgccat
     4921 cgaacgggcg cgctttgcgc atcccgtccc gctgatgcag ggattcgccg cgatcgaagc
     4981 gcagctcgcc cggctcgggc gcccacgcac cgcgctgtgc gcggccgagt tgcgctcgcc
     5041 caagccgttc accatggacg gtttcgccga gttcaatcga ggctacgtgg cggtgctgga
     5101 gcgatggggc ctgttccgcg accgccgcaa tccggtcgca cgctccaacg ttgcgccgca
     5161 gatcgacccg ccggccgagc cgtgcttcta cgcgttctgt tatacggtgc ccgccgccga
     5221 gaccgccccc ggcttcgtcg tcgccggcag cggcgaatgg cccgagggcg gccgcttccc
     5281 cgacgacatc gtcgcgcgcg gcgacgtctc acccgcaggc gtcgcggcca aggcgcgctg
     5341 cgtcctggac gcgatggagc ggcgcatggc gggcctgggt gtgtcgtggc gaaacgtgac
     5401 ggcaagccag gtgtacaccg tgcacgacat ccacgccgtg ctcggcaagg agatactgcc
     5461 gcgtgcgggc aacggcggcg ggctcacctg gcactactgc cggccgccga tcgaggaact
     5521 cgagtacgaa atggacgtgc gtggcgcaac ggtcgagcgg gttattcccg ccccgtagcc
     5581 cggatggagc ggagcgcact ccggggacca aaaccgcctc tctccccgga ttccgcttcg
     5641 ctgcatccgg gctacgccgg ggcgggttct gggcgccgag cgcgccgcca ggcttcgatc
     5701 gccccgccca ggatgccctt gggcatgaag atgatgaaca gcaccagcag caggccgtag
     5761 agcgtggtgt cgaggccgtg gatgtcgtgc cccagcgcca gcctcagcac ctcggccagc
     5821 agcagcgtga acaccgcacc cacggtcggc cccaggcgca cgtacatgcc gccggcaatc
     5881 gccccgaaga ccatctgcag gctgatgccg atgccggaca cggtctccgg gttgacgtag
     5941 agctgatact gagcgtacag cacaccgccg acgcacgtca tcagcgccga gaccagcgtc
     6001 acgccgagct tcaagcgcgt gacgtggatg cccacggacg cggcggcctc ctcctcgtgt
     6061 gcgatcgcct ccagcgccag ccgcgccatg gagcggtcca ctcgcgtcca cacccacagc
     6121 cccgcaagcc agaccacgag catgatgtag aaccagacga gcttcgagga gaactgcatc
     6181 gcccccaacg agaacgcctg ctcggcgagc gcggtgttcg gcgtggcgcc gagcgaaccg
     6241 ccggtctggt cgcgcagcgc gacgatgatc atgcgcacga cctcggacag cgccagcgtg
     6301 accaacgcga agtagtgtcc gacgatcttg aaacggaagc acggccagcc gatcaggaat
     6361 gccaccaccg cggtcagcgc cagcgcgatg ggcatcccca gccaggggga cagcccgaag
     6421 tggttccagc ccatcaccac cccgtaagcg ccgatgccga ggaacgcgcc gtggccaagg
     6481 ctcaccatgc ccagccgccc catcagcgcc cagccggtgt acacgaagcc ccacagcagg
     6541 cacatgatgg cgaggtgcag cgggtagttg ccgatcccgc tcagcggcgc cagcgcgagc
     6601 gccgccagcc cagcccaggg cagccagccg atcacgactt gcctccgaac aggccgcgcg
     6661 gcttccagaa catcaccgcg atgaagaaca gaaaggcgat cacgtaaccc cactcgaggt
     6721 ggaagaagta gccgcccacc gagatgaact gggcgaagat gaacgcggcg atgaagccgc
     6781 cgatcatgtt gcccagcccg cccagcacgc acaccaggaa agcgatgggc ccgaacgaca
     6841 gcccgatcgc cgggtggatg tcgtactgca gcaccagcag gcaggcggcc agccccgcca
     6901 gcccgccgcc cagcgccgag gtgatcagaa agatgcggtt gggattgaca cccatcagcg
     6961 gcatgatctc gcggtcctgg gagatcgcgc ggatggcggt gccgaggtaa ctgcgcttga
     7021 ggaacagata gacgccgatc atgcccgcga gcgccgccag gaaagcgatg aggcgcgcga
     7081 agctcacgaa catctcgccc acctccagcg tgggcagccg cacgccgatg ttcttgaact
     7141 ccacgccgaa ggcgagcgtg gccacgccct gcagcaggaa cagcacgccg ccggtgacca
     7201 gcagctggtt gatcggctcg gccgccagca ccgggcggat cacgtagtaa tgcagtgccg
     7261 cccccaggac cgacaccagc gccacgccgg cggcgaaggc cagccacagc ggccagccgt
     7321 acaccacgtg caggtagtac accgcgtaca tgccgatcat gatcagctcg gcgtagcaga
     7381 tccacaccac gtcgatcacg ccgaagatca ggttcaaccc cagcgccagc agcgccagga
     7441 tgccgccgag cagcacgccg ttgaccacgg cttcgagcag aaagatgtcg aagatgtcca
     7501 tcggtcacag gcccagataa gctttgcgga tggcctcgct cgcgagcagc tcggcggagc
     7561 tgccctggag tttgatgcgg ccggtttcga gcagataggc gcgatcggcc accttcagca
     7621 cctggcggat gttctgctcg acgatcagca ccgtgtagcc ctcggcgcgc atctgccgga
     7681 ccacctcgaa ggtcgcctgc accaccaccg gcgccagtcc ggccgacggc tcgtcgagca
     7741 ggagcagctt cggcccggac atgagcgcac gcccgatggc gcacatctgc tgctctccgc
     7801 ccgatagcgt accggcgggc tgccggcggc gctcctgcat gcgcgggaag tgccggtaca
     7861 cggcggcgag ccgctcgtcg aaacgcgccc gggccgcggg cgtgaacgcg cccatgcgca
     7921 ggttgtcctc gacgctcaag cgcgggaaca gccgccggtt ctccggcacg tgcgcgatgc
     7981 catggccgat cacctcgtgc gcgggaacgg cgttgagccg cttgccctcc atggtcatct
     8041 cgccggcgcg cggctcgatc aggcgcgaga tcacgcgcaa cagcgtggtc ttgcccgctc
     8101 cgttcggccc gatcacggcg acggtctcgc ccgctttcac ttccaggccg atgtcgaaca
     8161 gcgcctggaa gctgccgtat ccggcggata cgccctgcag gctgagcata tcaggtgcgc
     8221 tcatgtcagc cacgccgggc cgcgaccgtc gcctgcacgt cctgcgcgtc gctgccgaga
     8281 tagacctcga tcaccctggg atcgtgcgcc acctcctcgg gcttgccctg cgcgatcagc
     8341 tcgccgtggt cgagcaccac gcagcggtcc accacgcgca tcagcacgcc catgatgtgc
     8401 tccacccaga tgatggtgat ggcgcgctcg tcgcggatgc gggcaagcag gcccgcggcc
     8461 tggtccatct ccttctcgtc caggccgccg agcgac
//
LOCUS       DASPGE010000121         3897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_621083, whole genome shotgun sequence.
ACCESSION   DASPGE010000121 DASPGE010000000
VERSION     DASPGE010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621083"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3150)
                     /locus_tag="VD791_03820"
     CDS             complement(<1..3150)
                     /locus_tag="VD791_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXV07131.1"
                     /translation="MGEMRDLDGRYPALLADDGLAELLLGPGTHGDHSAIEDCNPAAA
                     ALFRCTRERLLGMRWAELLPTVQPDGSASHPGFERRMAAARGLPQSFLWHFQRADGSP
                     VEALVDLEFPAAETRTRLRLRDLSPLRLADQAVAESEERLRQVLENTSAAVFVKDLDG
                     VYLYINQRFCEMFGRTRSEMRGLRDVDIFPAQIAEQLRVDDRSVLQARGPLEFEEYLI
                     VNGTPGTYLAIKFPLLDSRGEPYAICGIATDITRRKRTEDALRSAALAVSTAEGETVF
                     QELTRYLATTLGVECSFIAACTSEEATHVRTLAVYTDLGYEENIEYSLPGTACGTVVG
                     QDFRLVASGVRALFPQDRMFRRLAIESYAAYPLNDSRGCALGLIAVLSRRPMRDRELT
                     ESMLKIFAARAAAEIERGKADEARRISERSYREMFEATEDAIFVHDWDTGAMVDANPK
                     ACETYGYSYDEIRRLTVADISSGEHPYTMQEAKRWLERAKAGERVRFEWHRRNRDGSL
                     HWDEVCLKPAVIAGEKRILAFTRDITQRKQAEEALRNAALAVSSVEGDSAFEDLVRQL
                     AATVGADLAFVARFVDGDASRMRTLAAWVDGKLVGNYEYSLAGTPCESVVGRQFLFFG
                     SGVQQVFRVDAPGIDSYAAFPLFDMQGRSVGLIAVADRKPMKDPALIESVLKIFAARA
                     ASEIERKDAERELRASEEQYRAIFHTSVDGMMVLDAQGRVVDANPACVGMFGYARDEL
                     LGQEPIALLVAPASREACAQLASQVTGGRPFHGECQATRRDGELLDIEVRGMQMHHQG
                     RPHVLAIVRDISARKRAEAERAQLEGQLRQAQKMEAIGHLTGGIAHDFNNILTSIMGY
                     LVLAVERQTEIGDARMGRYLDQAHVASTRARDLIQQMLTFSRGRRGEPRALALPPLVK
                     EAVKLLRSTLPSSIELDTELSVEVPAVLLDPVQVEQVLLNLCINARDAMSGAGQIRVR
                     VGTVDDRGRTCTSCRKPLPSGRLVELSVRDTGSGIRPEVIDRMFEPFFSTKEVGKGSG
                     MGLATVHGIVHEHGGHVIVD"
     gene            complement(3285..>3897)
                     /locus_tag="VD791_03825"
     CDS             complement(3285..>3897)
                     /locus_tag="VD791_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXV07132.1"
                     /translation="GEEMRAALARAVPDVVLLDVNLPGEDGLSLARYLRERHDLGIIM
                     VTAAGDVVDRIVGLEVGADDYIAKPFDPRELRARVRSVIRRVQQRAPQPGATAAAPVR
                     RVSVGACSLELTTHRLIGPDGEEIPLTHMEFDLLRVFTEHPNQVLTRDQLLTLTRNRE
                     WEPFDRSIDIRIARLRRKIEPDPENPRVIRTVRGAGYMFVPGV"
BASE COUNT          552 a         1384 c         1353 g          608 t
ORIGIN      
        1 gtccacgatc acgtggccgc cgtgctcgtg cacgatgccg tgcacggtgg cgagccccat
       61 gccactgccc ttgcccacct ccttggtgga gaagaacggc tcgaacatgc ggtcgatcac
      121 ctccggccgg atgccgctgc cggtgtcgcg caccgacagc tcgaccaggc ggccggacgg
      181 cagcggcttg cgacaggacg tgcaggtgcg gccgcggtcg tcgaccgtgc ctacgcgcac
      241 gcggatctgc cccgcgccgc tcatggcgtc gcgcgcgttg atgcacaggt tgagcagcac
      301 ctgctcgacc tgcaccggat cgagcagcac cgccgggacc tcgacgctga gctcggtgtc
      361 cagctcgatg ctggacggca gggtcgaacg cagcagcttc acggcttcct tgaccagcgg
      421 cggcagggcc agcgcgcgcg gctccccgcg ccggccgcgg ctgaaggtga gcatctgctg
      481 gatcaggtcg cgcgcccggg tggaggcgac gtgcgcctgg tccagatagc ggcccatcct
      541 ggcgtcgccg atttccgttt gccgctcgac ggccagcacc aggtagccca tgatgctggt
      601 caggatgttg ttgaagtcgt gcgcgatgcc gccggtgagg tggccgatcg cctccatctt
      661 ctgcgcctgg cgcagctgcc cctcgagctg cgcgcgctcg gcttcggcgc gcttgcgcgc
      721 gctgatgtcg cgcacgatgg ccagcacgtg cgggcggccc tggtggtgca tctgcatgcc
      781 gcgcacctcg atgtcgagca gctcgccgtc gcgccgcgtc gcctggcatt cgccgtggaa
      841 cggccggccc ccggtgacct ggctggcgag ctgcgcgcac gcttcgcggc tcgccggtgc
      901 gaccagcagc gcgatcggct cctggccgag cagctcgtcg cgcgcgtagc cgaacatgcc
      961 cacgcaggcc gggttggcgt cgaccacgcg cccctgcgcg tcgagcacca tcatcccgtc
     1021 caccgaggtg tggaagatgg cgcggtactg ctcctcgctc gcgcgcagct cgcgctccgc
     1081 gtccttgcgc tcgatctcgg aggcggcgcg cgcggcgaag atcttcagca ccgattcgat
     1141 cagcgccggg tccttcatcg gtttgcgatc ggccacggcg atcagcccga ccgagcgccc
     1201 ctgcatgtcg aacagcggga acgccgcgta gctgtcaatg cccggggcgt cgacgcggaa
     1261 cacctgctgc acgccgctgc cgaagaacag gaactgccgt cccaccaccg attcgcatgg
     1321 cgtgccggcg agcgagtact cgtagttgcc caccagcttg ccgtccaccc aggcggccag
     1381 cgtgcgcatg cgcgaggcat cgccgtccac gaagcgggcg acgaaggcga ggtctgcgcc
     1441 caccgtggcg gcgagctggc gcaccaggtc ctcgaacgcg ctgtcgccct cgacgctgga
     1501 caccgccagc gcggcgttgc gcagcgcctc ctcggcctgc ttgcgctggg tgatgtcgcg
     1561 cgtgaaggcg aggatgcgct tctcgcccgc gatcaccgcc ggcttcaggc acacctcgtc
     1621 ccagtgcagg ctgccgtcgc ggttgcgccg gtgccattcg aagcgcacgc gctcgccagc
     1681 cttggcccgc tccagccagc gcttcgcctc ctgcatcgtg tagggatgct cgccggagct
     1741 gatgtcggcc acggtgaggc gccggatctc atcgtagctg tagccgtagg tctcgcacgc
     1801 cttgggattg gcgtccacca tggcgccggt gtcccagtcg tgcacgaaga tggcgtcctc
     1861 ggtggcctcg aacatctcgc ggtagctccg ctcggagatg cggcgcgctt cgtcggcctt
     1921 gccgcgctcg atctcggcgg cggcgcgcgc ggcgaagatc ttgagcatgg actcggtcag
     1981 ctcgcggtcg cgcatcgggc ggcgcgacag caccgcgatc aggcccaggg cgcagccgcg
     2041 cgagtcgttc agcggatagg cggcgtagct ctcgatcgcc agccgccgga acatccggtc
     2101 ctgggggaac agcgcgcgca cgccggaggc gaccaggcgg aagtcctgtc ccaccacggt
     2161 gccgcaagcg gttccgggca gcgaatactc gatgttttcc tcgtagccca ggtcggtata
     2221 caccgcgagc gtgcgcacgt gggtggcctc ctcgctggtg caggcggcga tgaagctgca
     2281 ttccacgccc agcgtcgtgg ccaggtagcg cgtcagctcc tggaacacgg tctcgccctc
     2341 ggcggtggac accgccaggg ccgcgctgcg cagcgcgtcc tcggtgcgct tgcgccgggt
     2401 gatgtcggtg gcgatgccgc agatcgcata cggctcgccg cgcgagtcga gcagcgggaa
     2461 cttgatcgcc aggtaggtgc cgggcgtgcc gttgacgatc aggtactcct cgaactccag
     2521 cgggccgcgc gcctgcagca cgctgcggtc gtccacgcgc agttgttcgg cgatctgagc
     2581 ggggaagatg tccacgtcgc gcagcccgcg catctcgctg cgcgtgcggc cgaacatctc
     2641 gcagaagcgc tggttgatgt acagatagac cccgtccagg tccttgacga ataccgccgc
     2701 cgaggtgttc tccagcacct ggcgcaagcg ctcctcgctc tcggccaccg cctggtcggc
     2761 gagccgcaag ggcgacagat cgcgcaggcg caggcgcgta cgcgtctccg cggcggggaa
     2821 ctcgagatct accagagcct ccaccgggct gccgtccgcg cgctggaaat gccacaggaa
     2881 ggactgcggc aggccgcggg ctgcggccat gcgccgctcg aaaccggggt gcgaagcgct
     2941 gccgtcgggt tgcaccgtcg gcagcagctc cgcccaccgc atgcccagca ggcgctcgcg
     3001 ggtgcaacgg aacagcgccg ccgcggcggg gttacagtcc tctatcgcac tgtggtcgcc
     3061 atgcgtgccg gggccgagca gcagctcggc caggccgtcg tcggcgagca gggccggata
     3121 ccggccgtcc aggtcgcgca tctctcccat gatcacagcg gctacggatg gatgacgcca
     3181 gtctatcctc gtccgcgcgc gcgcgggcaa gttttacccg gccgatacca ttgaaacacc
     3241 gcggttacat tcgcccgccg gttatgttca cccgccgatt atgttcacac cccgggcacg
     3301 aacatgtacc ccgccccgcg cacggtgcgg atgacgcggg gattctccgg gtccggctcg
     3361 atcttgcggc gtagccgcgc gatgcggatg tcgatgctgc ggtcgaacgg ctcccactcg
     3421 cgattgcgcg tcagagtcag aagctggtcg cgggtgagca cctggttggg gtgctcggtg
     3481 aacacgcgca acaggtcgaa ctccatgtgc gtgagcggaa tctcttcgcc gtcggggccg
     3541 atcaggcgat gggtggtcag ctccagggag caggcgccca cgctcacgcg gcggaccggc
     3601 gcagccgctg tcgcccccgg ctggggcgca cgctgctgca cccggcggat cacgctgcgc
     3661 acgcgcgcgc gcagctcgcg cggatcgaac ggcttggcga tgtaatcgtc ggcacccacc
     3721 tcgaggccga cgatgcggtc caccacgtcg ccggcggcgg tgaccatgat gatgcccagg
     3781 tcgtggcgct cgcgcaggta gcgcgccagg ctcaacccgt cctcgccggg caggttgacg
     3841 tcgagcagca ccacgtccgg gaccgcgcgc gcgagcgccg cgcgcatctc ctcgccc
//
LOCUS       DASPGE010000122         3957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1440304, whole genome shotgun sequence.
ACCESSION   DASPGE010000122 DASPGE010000000
VERSION     DASPGE010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440304"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VD791_03830"
     CDS             <1..683
                     /locus_tag="VD791_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXV07133.1"
                     /translation="WRQWKIGVLLDAPNAEVDRSVQERIQGVAEFLARRGAKVDDRAR
                     PDIDLAEAHRVFLQLLRAATTRGLTDEMFVRHQEAARQLRPDDDSYYAWMVKANTMHH
                     RDWLYLNEKRHRMRLKWGEFFREHDLLLCPPAATPAFAHNQQGERWERMVSVNGKPQP
                     STTQMFWAGYSGMCYLPSTVAPAGFSAGLPVGVQIVGPQYGDLTCIAFAQLLERELQG
                     FVAPPGYD"
     gene            726..1637
                     /locus_tag="VD791_03835"
     CDS             726..1637
                     /locus_tag="VD791_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXV07134.1"
                     /translation="MTTPPYAWALFTVAAAAAQTARNAMQRGLTAKVGVAAASWARFI
                     FALPFALAFLAVVLSFETGSVSVRDGFGVWLLIGSLAQMAGTATMLLAMRDRSFVTVT
                     AYLKTEPIQVAAFSFAFLGEPLTPLRFAAVVLGTAAVMLMSWPPASTKDAFGAAGRDL
                     RGAGWGVAAAAAFAMAAVGYRGAVTHLDGVSFLSAATLALAATLTIQTLLSIAWLLIA
                     NRTSLPALFVAWRESVPAGFAGAIASQCWFLALALQAAPAVRTLALVEVVFAQLVSWR
                     LLREKVSRREIAGMALLAVALIVILRT"
     gene            1658..2185
                     /locus_tag="VD791_03840"
     CDS             1658..2185
                     /locus_tag="VD791_03840"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HXV07135.1"
                     /translation="MPIESLIRTIPHYPRPGVMFRDITTLLKDPVGFRLTVNELLRRY
                     RDQRIDKVAAIESRGFIIGAPLAFELGAGFVPLRKKGKLPAETVGQDYELEYGADRIE
                     MHVDAILREEHVLLVDDLLATGGTAEASCKLIESAGGRVVECCFVIDLPELGGRRRLL
                     DQGYAVHALVEFPGH"
     gene            complement(2189..>3957)
                     /locus_tag="VD791_03845"
     CDS             complement(2189..>3957)
                     /locus_tag="VD791_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXV07136.1"
                     /translation="ELSQTLEVMGARVVAASSSPLGWLTVVESPDVPLRHVPGLSLNA
                     PGEPPAQLALFTDGEGLTPITRFDGRREPLAYLDFVTSAAAYHVLDRPRVLVLGAGAG
                     ADVLQALAHSAASIDAVELNPAVARLVERDFAGFSGRPLSHPGVRLHIAEARAFATAS
                     RARYDLVQLALLDAFGASSAGLYALAESYLYTVESLRIYLRRLAPDGILSITRWVTLP
                     PRDTLKLFGMAALALEREGASRPGKRLVLLRSWRTATLLIKNGDWSDAQIERLKGFAA
                     QRSFDFGWYPGMTAADADRYNILDRPWFYEGATALLGPQRDAFVADYKFDIAPAIDDR
                     PYFFHFFRWRALPELLRLKGQGGLPLLEWGYPLLVATLLQAVLASAALILLPLWLLGR
                     RDRARSREADRWRVAVYFTAIGLAFMLVEIAFIQKFGLLLGHPLYAIAVVLFAFLLSA
                     GFGSRFSERLLPAGLRITLPVLAIAAVSGLYASLLLPVLLPVVAALPDAVRIAIGVAL
                     ILPLGFAMGMPFPAGLARVAASAERLVPWAWGVNACASVIGAIAATLAAVHFGFTAVL
                     IAAIALYALAAACAPRPVSNVD"
BASE COUNT          602 a         1406 c         1396 g          553 t
ORIGIN      
        1 cctggcggca gtggaagatc ggcgttctgc tcgacgcccc caacgcggag gtcgaccgca
       61 gcgtgcagga gcgcatccag ggcgtcgccg agttcctggc gcggcgcggc gccaaggtgg
      121 acgatcgcgc ccggcccgac atcgatctgg ccgaggcgca tcgcgtgttc ctccagctgc
      181 tgcgcgcggc gaccacccgc gggttgaccg acgagatgtt cgtgcgccac caggaggccg
      241 cgcgtcagtt gcggcccgac gacgacagct attacgcctg gatggtgaag gccaacacca
      301 tgcaccaccg cgactggctg tacctgaacg agaagcgcca tcgcatgcgc ctgaagtggg
      361 gcgagttctt ccgggagcac gatctgctgc tgtgcccgcc ggccgccacg ccggcctttg
      421 cccacaatca acagggcgag cgctgggaac gcatggtgag cgtcaacggc aaaccgcagc
      481 cgtccaccac gcagatgttc tgggccggct actcgggcat gtgctacctg ccctcgacgg
      541 tcgcgccggc cggcttcagc gccggcctgc cggtcggcgt gcaaatcgtc ggcccgcagt
      601 acggcgatct gacctgcatc gccttcgcgc agctgctgga gcgcgagctt cagggtttcg
      661 tcgcgccgcc cggctatgac tgaggccgtt ccctcccccg cgagcggggg agggtgaggg
      721 agcgcgtgac cacaccgccc tacgcctggg cgctgttcac cgtcgccgcc gcggccgcgc
      781 agaccgcccg caacgcgatg cagcgcgggc tcaccgccaa ggtcggcgtg gccgccgcaa
      841 gctgggcgcg cttcatcttc gcgctgccgt tcgcgctggc gttcctggcc gtggtcctgt
      901 cgttcgagac gggcagcgtg tccgtgcgcg acggtttcgg cgtctggctg ctgatcggat
      961 cgctggcgca gatggcgggc accgccacca tgttgctcgc catgcgcgac cgctcgttcg
     1021 tgacggtcac cgcctatctc aagaccgagc cgatccaggt cgccgccttc tcgttcgctt
     1081 ttctcggcga accgctcacg ccgctgcgct ttgcggccgt ggtgctgggc accgccgcgg
     1141 tgatgctgat gtcctggccg cccgcctcga cgaaggacgc cttcggcgcg gccggccgcg
     1201 acctgcgcgg ggcgggctgg ggcgtggcgg ccgctgccgc cttcgccatg gccgcggtcg
     1261 gctatcgcgg tgcggtcacg catctcgatg gcgtgtcctt cctgtccgcg gcaacgctcg
     1321 ccctggccgc gaccttgacg attcaaactc tgctgtcgat cgcttggctg ctgatcgcga
     1381 atcggacatc cctgcccgcc ctgttcgtgg cctggcgcga gtcggtgccc gccggctttg
     1441 ccggtgcgat cgcatcgcaa tgctggttcc tcgcgctcgc cttgcaggcg gcgccggcgg
     1501 tgcgtaccct ggcgctggtg gaggtcgtgt tcgcgcagct cgtctcctgg cgcctgctgc
     1561 gcgagaaggt ttcccggcgc gagatcgcgg gcatggcgct gctcgccgtg gcgctgatcg
     1621 tcatcctgcg cacctgacgc gaccccggag cgcgctcatg cccatcgaat cgctgatccg
     1681 taccatcccg cactatccgc ggcccggagt gatgttccgc gacatcacca cgctgctcaa
     1741 ggacccggtc gggttccgcc tgacggtgaa cgagttgctg cgccgctacc gcgaccagcg
     1801 catcgacaag gtcgcggcca tcgagtcgcg cggtttcatc atcggcgcgc cgctcgcctt
     1861 cgagctgggc gccggcttcg tgccgctgcg caagaaaggc aagctgccgg cggaaaccgt
     1921 cggccaggac tacgagctcg agtacggcgc agaccgcatc gagatgcacg tggacgcgat
     1981 cctgcgggag gagcacgtcc tgctggtgga cgacctgctc gccaccggcg gcaccgcgga
     2041 ggcgtcgtgc aagctgatcg agagcgccgg cgggcgggtc gtggagtgct gcttcgtgat
     2101 cgacctgccc gagctgggcg ggcgccgccg gctgctggac cagggctacg cggtgcacgc
     2161 gctggtggag ttcccggggc actgaccgct agtccacatt gctcacgggg cgcggtgcgc
     2221 acgccgcggc caacgcgtac agcgcgatcg ccgcgatgag cacggcggtg aagccgaagt
     2281 ggaccgcggc cagcgtggcg gcgatcgcgc cgatcaccga tgcgcaggca ttgacgcccc
     2341 aggcccaggg cacgagcctc tcggcggatg cggcaacgcg cgccagcccc gccggaaacg
     2401 gcatgcccat ggcgaaaccc agcggcagga tcagcgcgac gccgatggcg atgcgcaccg
     2461 cgtcgggcag cgccgccacg accggcagca agacgggcaa caacagagac gcgtacaagc
     2521 cggacactgc ggcgatcgcg agcaccggca gcgtgatgcg caggcccgcg ggcaacagcc
     2581 gttcggagaa ccggctgccg aacccggccg agagcagaaa ggcgaacagc accacggcga
     2641 tggcatagag cgggtggccg agtaacagcc cgaacttctg gatgaaggcg atctcgacca
     2701 gcatgaacgc caggccgatc gccgtgaagt acaccgccac ccgccaccga tccgcctccc
     2761 gcgagcgggc gcgatcgcgc cggccgagca gccaaagcgg cagcaggatc agcgcagcgc
     2821 tggcgagaac ggcctgcaac agggtcgcca ccagcagcgg atagccccac tcgagcagcg
     2881 gcaggccgcc ctgccctttc aggcgcagca gctccggcag cgcccgccag cggaagaagt
     2941 ggaagaagta gggccggtcg tcgatggcgg gggcgatgtc gaacttgtag tccgcgacaa
     3001 aggcgtcgcg ctgcggcccg agcagcgcgg tggcgccctc gtagaaccag ggccggtcca
     3061 ggatgttgta gcggtcggcg tccgcggccg tcatgcccgg ataccagccg aagtcgaagg
     3121 agcgctgcgc cgcgaacccc ttcaggcgct cgatctgcgc atcgctccag tcgccgttct
     3181 tgatcagcag cgtggccgtg cgccagctgc gcagcagcac caggcgcttg cccggccggc
     3241 tcgcgccctc gcgctccagc gccagcgccg ccatgccgaa cagcttgagc gtgtcgcgcg
     3301 gcggcagcgt cacccatcgc gtgatggaaa ggatcccgtc gggggcgagc cgccgcaggt
     3361 agatgcgcag cgactcgacg gtgtacaggt agctctccgc cagcgcgtac agccccgcgg
     3421 acgaagcgcc gaaggcgtcc agcagcgcca gctgcaccag gtcgtagcgc gcgcgggagg
     3481 cggtcgcgaa ggcgcgcgcc tcggcgatgt gaagccgcac acccgggtgc gacagcggcc
     3541 ggccggagaa accggcgaag tcccgctcga ccagacgcgc cacggccgga ttcagctcca
     3601 ccgcatcgat cgacgcggcc gagtgagcca gcgcctgcag cacgtcggcg ccggcgcccg
     3661 cgcccagcac cagcacgcgc gggcggtcca gcacgtggta ggcggccgcc gaggtgacga
     3721 agtcgaggta ggcgagcggc tcgcgcctgc cgtcgaaccg ggtgatcggc gtcagaccct
     3781 cgccgtcggt gaacagcgcg agctgcgcgg gcggctcgcc cggcgcgttg aggctcaagc
     3841 ccggcacgtg gcgcaacggc acgtccggac tttccacgac ggtgagccaa ccgagcggac
     3901 tggagctcgc cgcgaccacg cgcgcgccca tcacttcgag cgtctggctg agctcct
//
LOCUS       DASPGE010000123         6903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_504805, whole genome shotgun sequence.
ACCESSION   DASPGE010000123 DASPGE010000000
VERSION     DASPGE010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504805"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VD791_03850"
     CDS             complement(<1..336)
                     /locus_tag="VD791_03850"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HXV07137.1"
                     /translation="MDLRATPNLVHLSNTASDRRLLELTPATGYRVVALSGEVWITQA
                     GQIADYVLRPGDALTLDSPGTAIVTSFGPADIEVIAPPAAAPLEWPPAISAEVVEQAQ
                     RRAHELRAQV"
     gene            754..1095
                     /locus_tag="VD791_03855"
     CDS             754..1095
                     /locus_tag="VD791_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07138.1"
                     /translation="MKSLFARKQLAVAIGLGASALAFGVYAEDLGKIKVTHDPAVAER
                     TNMQRPGDDSSDLKVSHDQAVADRTNMGRKPGDVGTVTTAPDYGLRARTNMGGIAKQG
                     AEPPVTAAAPK"
     gene            1212..1421
                     /locus_tag="VD791_03860"
     CDS             1212..1421
                     /locus_tag="VD791_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07139.1"
                     /translation="MRHRASIAGILAVHLAGALLLVATGAQAAGAPQRAHLHRPQQTS
                     LGLGDSKCILVGEVLKCVPTIVASG"
     gene            1537..2202
                     /locus_tag="VD791_03865"
     CDS             1537..2202
                     /locus_tag="VD791_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXV07140.1"
                     /translation="MDIQQVRRKLIELYPAFREMPAELLQYTLDNALVRTVPAGTVMF
                     DERNPCQAFPMILEGGIRVSKIAPNGRELQLYRVGPGESCILSTSCMLGTTPYTARGV
                     ADSEMTVFALPAPAFNHLISQHQPFRAYVFGLFADRIVDLMQLVEAVAFHKLDQRLAA
                     LLLGKGKVIHVTHQGLADELGSVREIVSRLLKSFAEQGMVSLGREQIEIVDAGRLRQL
                     AAR"
     gene            2315..2527
                     /locus_tag="VD791_03870"
     CDS             2315..2527
                     /locus_tag="VD791_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HXV07141.1"
                     /translation="MKQNVGSIDKAVRVIVGIGLLSLLFVLEGGARWFGLIGLVPLAT
                     AVFGYCPLYAVLGMNTCPAGGKPGHA"
     gene            complement(2556..2858)
                     /locus_tag="VD791_03875"
     CDS             complement(2556..2858)
                     /locus_tag="VD791_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07142.1"
                     /translation="MKTGLHELTERRETLVAQSAAQRAQLAQAVEGVQRGFMVGRLAL
                     DAWLIFKSQPIAAALAVGLVLAARPRRLLAWASTGLTVYSAARRIAAALRARRSGR"
     gene            complement(2855..3256)
                     /locus_tag="VD791_03880"
     CDS             complement(2855..3256)
                     /locus_tag="VD791_03880"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HXV07143.1"
                     /translation="MAEQPPRDQGERRAGLVGSLRALAATLVAIAHTRLELVGVELRE
                     EFARLGAVLLWGYLALFCGSVAMVLLTLALAVLWAEHRLIVLGVAAALFLVLALLAAR
                     AAVAVARRKPRLFEASLTELEHDNRQLRDRP"
     gene            complement(3293..3595)
                     /locus_tag="VD791_03885"
     CDS             complement(3293..3595)
                     /locus_tag="VD791_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HXV07144.1"
                     /translation="MDTIDRNKLLDDLRVVASDVEELLRATASQTGDRIKEARARAEE
                     SLRTARARLEETGDEVAAHAREAAREAERYVHDNPWQSVGIAAGIGFLLGYLIGRR"
     gene            3747..4424
                     /locus_tag="VD791_03890"
     CDS             3747..4424
                     /locus_tag="VD791_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXV07145.1"
                     /translation="MDDAAGRGELVVLVHGLWMGRAIWLFLARRLRRQGYRVASFAYP
                     SVRQGLTESALALEAFVRARPAIRLHLVGHSLGGLVVLRMLRDARSAALARVVLLGCP
                     VAGCSAAGQLGRSRTGRWLLGAALPQWTPAEAPPTVRRFETGAIAGTRRFGVGSVLLR
                     LPRLNDGVVMVDETVLPGMADHLVLPVSHSGMVISGRVADEVGTFLRTGRFAHRSQAI
                     ARATPLV"
     gene            4433..5521
                     /locus_tag="VD791_03895"
     CDS             4433..5521
                     /locus_tag="VD791_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HXV07146.1"
                     /translation="MSIAARRWYRLLPLIALSALLVAGAGCSTLGYYWQAFGGQMEMT
                     RKARPIPEVIADPATAGELKLRLERVHEIRDFASRALALPDNGSYRRYADLKRHFVVW
                     NVFATPEFSVQAREWCFPVAGCVGYRGYFAQADAEAFAAGLQREGLDVYVGGVPAYST
                     LGWLDDPVLNTFIGYPDTELARLIFHELSHQVAYAKGDSTFNESFAVAVETEGVKRWI
                     DAKGTPEQRAAFDASQQRRQDFAALVQRHRDALAQLYAGGLPPERMRAEKARALEAMR
                     ADYAALKQRWGGFAGFDEWFAQPLNNAQLASVAIYTQLVPGFERLLAGSDGDLPRFYA
                     EVKALAKLPERERRAALGAPPAERVTAR"
     gene            5594..6088
                     /locus_tag="VD791_03900"
     CDS             5594..6088
                     /locus_tag="VD791_03900"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXV07147.1"
                     /translation="MPIHSLIPRFLLLLLAFAAANAFAGPDVGEPAKLKSVALDGKPL
                     SLERLKGKVVMVNFWATWCVTCKAEMPMWQAFYDANKARGFEIVAMSIDEDQDELVKH
                     AQEKKFGFPIAWRWDDKTDDNFGDIIGTPTLYVVDKAGKVAWMKRGRVTPEQLAAVVE
                     PLLK"
     gene            6096..6569
                     /locus_tag="VD791_03905"
     CDS             6096..6569
                     /locus_tag="VD791_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXV07148.1"
                     /translation="MNEDINTLRRILKQSRTIAVVGLSADWWRPSYFAAKYMQEHGYR
                     VIPVNPSYQQILGEKCYASLRDVPQPVDIVDCFRKSEDIDPIAEDAIAIGAKVLWMQI
                     GVINHAAAEKARAAGLEVVMDRCVKIEHARLFGGLNWVGVNTKVISSKRPHWLAY"
     gene            complement(6606..>6903)
                     /locus_tag="VD791_03910"
     CDS             complement(6606..>6903)
                     /locus_tag="VD791_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV07149.1"
                     /translation="SLAVKPDGTADAACLTFSVSDTGIGLTPEQVGRLFQDFAQADAA
                     TTRKYGGTGLGLALSQRLCRLMGGDITVQSEAGRGSTFTARLPANVAAPARIER"
BASE COUNT         1055 a         2343 c         2417 g         1088 t
ORIGIN      
        1 gacctgggcg cgcagctcgt gcgcccggcg ctgcgcctgc tcgacgacct cggcgctgat
       61 cgccggtggc cactccagcg gcgccgcagc gggcggtgcg atgacctcga tatcggccgg
      121 accgaaggaa gtgacgatcg ccgtacccgg cgaatccagg gtcagcgcgt cgccggggcg
      181 cagaacgtag tccgcgatct gtcccgcctg cgtgatccag acctcgccgg acagcgcgac
      241 cacccgatat ccggtggcag gggtgagttc cagcagacgc cggtcggaag cggtattgct
      301 cagatgaacc aggtttgggg ttgcgcgaag atccatgatg tgctccgttc aggtatgatt
      361 gcggcgcatg caaaacgcgc cgccaagacg ggcgcgtctg tgcgctcatg aagtgcatta
      421 tcggcaccgg acggcagcgc caccagcgat ctttgcgcat gccaggcatg cgctacagga
      481 atgtctaaac ggggcgaccg gtgcggcgtc gagccttgac gaccggttga ttaccgcccc
      541 tttcaacagc taccaaaggt tctcgggcgt gatcggaatg tcgatgttgc cccgcgttta
      601 cggcgacgcg gggcggcgcg cctatgcgta ccctatttgt caggaccccg cgtgcagaat
      661 gggccacgtc agccggcgca tcgagcgact ccagcgcggc ggcggcgatt cggtaagcga
      721 tctgtacgta tcaactttgc aatgggggaa gtcatgaaga gtctgttcgc acgcaagcag
      781 cttgcagtag ccatcggtct gggcgcgtcc gcgctcgcct tcggggtgta cgccgaggac
      841 ctgggcaaga tcaaggtgac ccatgacccg gcggtcgccg agcgcaccaa catgcagcgg
      901 ccgggcgacg acagcagcga tctcaaggtc tcgcacgacc aggccgtggc cgatcgcacc
      961 aacatgggcc gcaagccggg cgacgtgggc accgtgacca ccgcgccgga ctacgggctg
     1021 cgggcgcgca ccaacatggg cggcatcgcc aagcagggag ccgaaccgcc cgtgaccgcg
     1081 gccgcaccga agtgagcgcc gcgggccacc ttccggttgc gtaggtgggt ctcctcctgc
     1141 gttgcggccc cgcccccgcg gggccgtttt ttccttccgg gtgggccggc ccgaagccgc
     1201 cgcgagccgc catgagacac agggcatcga tcgccgggat cctcgccgtt catctggcgg
     1261 gtgcgctgct gctcgtcgcg acgggcgcgc aggccgccgg cgcgccgcag cgcgcgcatc
     1321 tccatcggcc gcagcagact tcgctgggcc tgggggactc gaagtgcatc ctggtcggtg
     1381 aagtgctgaa gtgcgtgccg acgatcgtcg cgtccggcta gccgcccgca ttaccgacgg
     1441 acgaacgccg cttttctccc gccgctgcgc tgcaaccgga ttgcgtctgc tgcttccgct
     1501 gcgcgcggtg cgcaatcggg ctatgcttgc ctggccatgg acattcagca ggtcaggcga
     1561 aagctgatcg aactgtatcc ggccttccgg gaaatgcccg cggaattgct gcaatacacc
     1621 ctcgacaatg cgctggtgcg cacggtgccg gcgggaaccg tgatgttcga cgagcgcaac
     1681 ccatgccagg catttcccat gatccttgaa ggcgggatcc gcgtatccaa gatcgccccg
     1741 aacggccgcg agctgcagct ctaccgggtg ggcccgggcg agagctgcat cctgtcgacg
     1801 agctgcatgc tgggcaccac gccttacacg gcgcgcggcg tggccgattc ggagatgacc
     1861 gtgttcgcgc tgccggcccc cgccttcaac catctgatct cgcagcacca accgttccgc
     1921 gcttacgtct tcgggctgtt cgccgatcgc atcgtggacc tgatgcagct ggtggaggcg
     1981 gtggcgttcc acaagctgga ccagcggctc gccgcgctgc tcctgggcaa gggcaaggtg
     2041 atccacgtca cccaccaggg gctggccgac gagctgggca gcgtgcgcga gatcgtcagc
     2101 cggctgctca agagcttcgc cgagcagggg atggtgtcgc tcggtcgcga gcagatcgag
     2161 atcgtcgatg ccgggcggct gcggcagttg gccgccaggt gacttcggtc acagagccgc
     2221 gcagcctggt gtctctatga tgacaagagg tgccgcaagc acccgcgtca accccggaaa
     2281 gcgccgtgtc aaccctggag agcaaggagg ccctatgaaa cagaatgtcg ggagcatcga
     2341 caaagcggta cgcgtgatcg taggcatcgg cctgctcagc ctgttgttcg tgctggaagg
     2401 cggagcgcgg tggttcgggc tgatcggcct ggtgccgctc gccaccgcgg tgttcggcta
     2461 ctgcccgctg tatgcggtgc tgggcatgaa cacctgtcct gccggcggca agcctggaca
     2521 cgcctgacgc gtcgcgccgc ggcgcgcgcg cggcgctagc gccctgaccg gcgggcgcgc
     2581 agggcggctg cgatacggcg tgcggccgag taaacggtga gcccggtgct cgcccaggcc
     2641 agcagccgcc gtggtcgcgc cgcgagcacg aggccgacgg cgagcgcggc cgcgatcggc
     2701 tgcgatttga agatcagcca ggcgtcgagc gccaaccgcc cgaccatgaa cccgcgctgt
     2761 acaccctcga ccgcctgcgc gagctgcgcg cgctgagccg cggactgcgc gaccagcgtc
     2821 tcgcgtcgtt cggtcagctc gtgcagcccg gttttcatgg gcggtcgcgc agctgccggt
     2881 tatcgtgctc gagctcggtc aggctggctt cgaacagccg cggcttgcgg cgcgccaccg
     2941 cgaccgcggc gcgcgccgcg agcagcgcca gcacgagaaa cagcgcggcc gccacgccga
     3001 gcacgatcag ccggtgctcg gcccagagga ccgcgagggc gagcgtgagc agcaccatcg
     3061 caacgctgcc gcagaacagc gccaggtatc cccacagcag caccgcgccc aggcgcgcga
     3121 actcctcgcg cagctcgacg ccgaccagct cgagccgggt gtgcgcgatc gcaaccagcg
     3181 tggcggcgag cgcccgcagc gaaccgacca ggcccgcacg gcgttcgccc tggtcccgcg
     3241 gcggctgttc ggccatggct ctggggaaag gggcctcgat gacaaccggg gcctagcggc
     3301 gcccgatgag atagccgagc aggaagccga tgccggcggc gatgccgacc gactgccacg
     3361 gattgtcgtg cacgtaccgc tccgcctcgc gcgccgcctc ccgggcatgc gccgcgacct
     3421 cgtcgccggt ctcctccagg cgcgcgcgcg cagtgcgcag cgactcctcg gcgcgcgcgc
     3481 gcgcctcctt gatgcgatcg ccggtctgac tggcggtcgc gcgcagcagc tcttccacgt
     3541 cgctcgccac gacccgcagg tcgtcgagca gcttgttcct gtcgatggtg tccacgtgat
     3601 cctccaaagc gttggcgcgc aggcaaagcg ttggcgccgg gcaaagtgat ggcgcgttac
     3661 gatgaagcgt ttcggctcga tggtagcccg cgccgccgcc ggccgcaacc cgcgagccac
     3721 ggccatcgcg ctatagaatg ccgcggatgg acgatgcggc cggacgcggc gagctggtgg
     3781 tgctggtgca cgggctgtgg atggggcgcg cgatctggct gtttctcgcc cggcgcctgc
     3841 gtcggcaggg ctatcgggtc gcgtccttcg cgtacccgtc cgtgcgccag gggctcaccg
     3901 agagcgcact tgcactcgag gcctttgtac gggcgcgtcc tgcaatccgc ctgcatcttg
     3961 tcggacacag cctcggcggg ctggtggtgc tgcgcatgct gcgcgatgcg cgctctgcgg
     4021 ccctcgcgcg ggtggtcttg ctcggctgcc cggtggcagg ttgcagcgcc gcggggcagc
     4081 tcggccgcag caggaccggc cgctggctgc tgggcgcagc gctgccgcag tggacgccgg
     4141 cggaggcgcc gccgacggtg cggcgtttcg agaccggcgc gatcgccggc acgcgccgct
     4201 tcggcgtggg ctcggtgctg ctgcggctgc cgcgcctcaa tgacggcgtg gtcatggtcg
     4261 acgagacggt tctgcccggg atggccgatc acttggtgct gccggtcagc cattcgggca
     4321 tggtgatctc ggggcgcgtg gcggacgaag tggggacttt cctgcgcacc gggcgctttg
     4381 cgcaccgatc ccaggccatc gcacgcgcca cccccctcgt ataatcggcc cgatgtccat
     4441 cgctgcgcgc cgctggtacc ggctcctgcc gctgatcgcg ctgtccgcct tgcttgtagc
     4501 gggggccggc tgcagcacgc tcggctacta ctggcaggcg ttcggcggcc agatggagat
     4561 gacgcgcaag gcgcgcccga tcccggaggt gatcgccgac cccgccaccg ccggcgaact
     4621 caagctcagg ctggagcggg tgcacgagat ccgcgacttc gccagccgcg ccttggcgct
     4681 gcccgacaac ggcagctatc gccgctacgc agatctgaag cggcacttcg tcgtgtggaa
     4741 cgtgttcgcc acgccggagt tctcggtgca ggccagggag tggtgcttcc cggtggccgg
     4801 atgcgtgggc tatcgcgggt actttgcgca ggccgacgcc gaggcgttcg ccgccggctt
     4861 gcagcgcgag ggcctggatg tatacgtggg cggggtgccc gcctattcca cgctcgggtg
     4921 gctggacgat ccggtgctca acaccttcat cggctatccg gacacggagc tggcgcgctt
     4981 gatcttccat gagctgtcgc atcaggtggc ctacgccaag ggcgacagca cgttcaacga
     5041 gtcgttcgcg gtggcggtgg agaccgaggg cgtcaagcgc tggatcgacg cgaagggcac
     5101 gccggaacag cgtgcggcgt tcgacgcgtc ccagcagcgc aggcaggact tcgccgcgct
     5161 ggtgcagcgc catcgcgatg ccctcgcgca gctctacgcg ggcggcctgc cgccggagcg
     5221 gatgcgcgcc gagaaggcgc gcgcgttgga agcgatgcgc gccgactacg ctgcgctcaa
     5281 gcagcgctgg ggcggcttcg ccggcttcga cgagtggttc gcgcagccgc tcaacaatgc
     5341 gcagctcgcc tcggtcgcca tctacacgca gctggtgccc ggcttcgagc ggctgctcgc
     5401 cggcagcgac ggcgatctgc cgcggttcta tgccgaggtg aaggccctgg ccaagctgcc
     5461 cgagcgcgag cggcgcgcgg cgctcggcgc gccgcccgcg gagcgcgtca ccgcgcgctg
     5521 aagcgtataa tccgacgcat ccgttgcggc cgggctggcc gcccgtcttt tctctccttc
     5581 cggtgccctg accatgccga tccatagcct gatcccgcgc ttcctgctgc tcctgctcgc
     5641 gttcgcggca gccaacgcct tcgccggccc cgacgtcggc gaaccggcca agctcaagag
     5701 cgtcgccctg gacggcaagc cgctgtccct ggagcggctc aagggcaagg tggtgatggt
     5761 gaacttctgg gccacctggt gcgtgacctg caaggcggag atgcccatgt ggcaggcctt
     5821 ctacgacgcc aacaaggcgc gcggcttcga gatcgtggcc atgagcatcg acgaggacca
     5881 ggacgaactc gtcaagcacg cgcaggagaa gaagttcggc ttcccgatcg cctggcgctg
     5941 ggacgacaag accgacgaca acttcggcga catcatcggc acgcccacgc tgtacgtggt
     6001 ggacaaggcc ggcaaagtgg cgtggatgaa gcgcggccgg gtgacgcccg agcagcttgc
     6061 cgcggtggtg gagccgctgc tcaagtaggt ccgggatgaa cgaagacatc aacacgctgc
     6121 ggcgcatcct caagcagagc aggaccatcg ccgtggtcgg gctgtcggcc gactggtggc
     6181 ggccgagcta cttcgccgcc aagtatatgc aggagcacgg ctatcgcgtc atcccggtca
     6241 acccgagcta ccagcagatc ctcggcgaga agtgctacgc cagcctgcgc gacgtcccgc
     6301 agccggtgga catcgtggac tgctttcgca agagcgagga catcgacccg atcgcggagg
     6361 acgccatcgc catcggcgcc aaagtgctgt ggatgcagat cggcgtgatc aatcacgccg
     6421 cggccgagaa ggcgcgcgcc gccgggctgg aggtggtgat ggaccgctgc gtgaagatcg
     6481 agcacgcgcg gctgttcggc gggctgaact gggtgggagt caataccaag gtcatctctt
     6541 cgaagcgccc gcattggctg gcttactaga gggtcggatc ggcgtaacgc cgcgtttctc
     6601 tatcctcacc gctcaatgcg cgccggggcc gcgacgttcg cgggcagccg cgccgtgaag
     6661 gtactgcctc ggcccgcttc gctctgcacg gtgatgtctc cacccatcaa tcggcacagc
     6721 cgctggctca gcgccagtcc caggcccgtg cccccgtact tgcgtgtggt cgcggcgtcc
     6781 gcctgcgcga agtcctggaa cagccgtccc acctgctccg gcgtcaggcc gatgcccgtg
     6841 tcgctcacgc tgaacgtcaa gcaggctgcg tccgcggtgc cgtccggctt gaccgcgagc
     6901 gaa
//
LOCUS       DASPGE010000124         5277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_972855, whole genome shotgun sequence.
ACCESSION   DASPGE010000124 DASPGE010000000
VERSION     DASPGE010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972855"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="VD791_03915"
     CDS             <1..1355
                     /locus_tag="VD791_03915"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HXV07150.1"
                     /translation="VLHTLNLRLAPDEIGYIANHARDRFLIVDDILLSLFERFRGQVN
                     LERVIVVPLTGKPVPAGTDDYEAFLRTATGEFTYPPMDENEPCAMCYTSGTTGRPKGV
                     VYSHRSQLLHSLVQAMPDALGMSGRDVILPVVPMFHANAWGVPYTAAMLGVKMVFPGP
                     HLHPEDLIPLLESEKVTISMGVPTIWLPMVQLLEANRGRWKLQPNLRLTVGGSAVPEA
                     MIRAYARLGIEVIQGWGMTETSPLATVAKITALQEHLSEDERFAVLASQGYPLPLVDI
                     RAVSDQGEQPWDGKSVGEIQVHGPWITGRYHEQPAPENFAPDGWLRTGDVASLDPQGF
                     LKITDRTKDLIKSGGEWISSVDLENAIMAHPAVQEAAVIAVPHPKWAERPLAVIVLKP
                     GASATEADIRSHLAQRFVKWMVPDAYAFVDAIPRTSTGKFLKTRLREQYRDWKWAEQT
                     "
     gene            1352..2488
                     /locus_tag="VD791_03920"
     CDS             1352..2488
                     /locus_tag="VD791_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018026687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXV07151.1"
                     /translation="MNFEPSDEQKLIKHTAREFARKEIAPRAAACDREARFPMEVQQQ
                     ARDIGLVNMTVPREHGGSGWGVLDQALVTAELAWGCTGITGALGLNAIFGDVFQVAGS
                     VEQRKRAFGRLLAGEFGAYAVTEPAAGSDVARIKTRAVRKADRYVLNGSKVWISNAPL
                     ASMFVAFAKTDPEAGHKGISAFFVDRDTPGLRVGPPLGKLGQKASPAAEVFLEDVEVP
                     VSALIDAEGAGFLIAMRVFDRSRPMIAALALGLMQRCLDEALQYARTRQTMGQPIIEH
                     QAIGHKLADMALRTEAARLLTEQAAWMLDTGRRNTLQAAYAKTFAADSAMWAATEAVQ
                     IFGGMGYSTEYPVEKLFRDAKVLQIYEGTSEIQRNIIARELGRQ"
     gene            2507..3682
                     /locus_tag="VD791_03925"
     CDS             2507..3682
                     /locus_tag="VD791_03925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXV07152.1"
                     /translation="MAEAVIVDAVRTPFGRRGGALREQRPDAMLAHALKAVVRRAGVD
                     ATRIEDVVDGTVTLAGEQGANPGRLGVLLAGFPVEVPGVSLNRMCGSSQQALHFAAQA
                     VAAGDMNYVIASGVESMTRVPMFLDISLGREGVHGFEVLNPELFRRHELIHQGESAER
                     IAEKWGLSRQAVDEFAKESHRRAARAQQAGWNREIVPIEGVSPEAQKIEVVRDEGVRA
                     VLDEQKIAALKPAFRPEGKGVVTAANSSQISDGASAVLVANADAAKADGVKARARFRA
                     RVVVGSDPTMQLTGVIPATRKALQRAGLSVRDIDWFEINEAFAVVPLCWAQDLGIPLE
                     KVNPWGGAIAHGHPLGATGGGLMAKMLAGLEATGGRLGLQVMCIGHGQATATIVERV"
     gene            3796..4563
                     /locus_tag="VD791_03930"
     CDS             3796..4563
                     /locus_tag="VD791_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07153.1"
                     /translation="MFRSFRILVLLLVLFTVALGNWLARARSTSWEHSLRMVVFPIDA
                     DGSPATARYIASLSRETFAPIDAFMASEAQRYGLALRDPLDVDVAPPVTRLPPAVPHG
                     GNALQIVLWSLRLRYWAWVNAEYPGPTPQVRMFVLYHDPDRVSRLNHSLGLQKGLIGV
                     VNAFAADAQAGPNNVIIAHEMLHTLGATDKYDPLTNYPLHPIGFAEPELAPVVPQRFA
                     EIMGGRVPMSRADARIPDDLDEVLIGAATALEIRWLQ"
     gene            4606..>5277
                     /locus_tag="VD791_03935"
     CDS             4606..>5277
                     /locus_tag="VD791_03935"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXV07154.1"
                     /translation="MATDHPKGLRRNLTSYGDRDFALYLRRSFARAMGYSLDLLDRPI
                     VGIAQTGSGFNNCHRTMPELVEAVKRGVLAGGALPLDFPTVSLGEIFLSPTSLMFRNL
                     MSMDTEEMIRAQPMDAAVLVGGCDKTVPAQLMGAASADLPAVQLVTGPMMAMPWHGER
                     LGACTDCRRFWARHRAGEIDAAGIAEVEGNLATTAGTCAVMGTASTMACIAEALGMAL
                     RDSAAI"
BASE COUNT          821 a         1785 c         1894 g          777 t
ORIGIN      
        1 gcgtgctgca cacgctgaac ctgcggcttg ctcccgacga gatcggctac atcgccaacc
       61 acgcgcggga ccgcttcctg atcgtggacg acatcctgct gtcgctgttc gagcggttcc
      121 gcggccaggt gaacctggag cgggtgatcg tggttccgct caccggcaaa ccggtgccgg
      181 ccgggacgga cgactacgaa gcgttcctgc gcaccgccac cggcgagttc acgtacccgc
      241 cgatggacga gaacgagccg tgcgcgatgt gttacacctc cggtaccacc gggcggccga
      301 agggcgtggt gtactcgcac cgctcgcagc tgctgcactc gctggtgcag gccatgcccg
      361 atgcgctcgg catgtcgggg cgcgacgtga tcctgccggt ggtgccgatg ttccacgcca
      421 acgcctgggg cgtgccctac accgcggcga tgctgggcgt gaagatggtc ttcccgggcc
      481 cgcacctgca tcccgaggac ctgatcccgc tgctggagag cgagaaggtc accatctcca
      541 tgggcgtgcc caccatctgg ctgccgatgg tgcagctcct cgaggccaac cgcggccgct
      601 ggaagctgca gccgaacctg cgcctcaccg tgggtggatc ggcggtgccg gaggcgatga
      661 tccgcgccta cgcccggctc ggcatcgagg tgatccaggg ctggggcatg accgagacct
      721 cgccgctcgc caccgtggcc aagatcaccg ccctgcagga gcacttgagc gaggacgagc
      781 gcttcgcggt gctcgcgagc cagggttatc cgctgccgct ggtggacatc cgcgcggtaa
      841 gcgaccaggg cgagcagccg tgggacggca agagcgtggg cgagatccag gtgcacggtc
      901 cttggatcac cggccgctat cacgagcagc cggcgccgga gaacttcgcg cccgacgggt
      961 ggctgcgcac cggggacgtc gcctcgctcg atccgcaggg cttcctgaag atcaccgacc
     1021 gcaccaagga cctgatcaag tccggcggcg aatggatcag ctcggtcgac ctggaaaatg
     1081 cgatcatggc gcaccccgcg gtgcaggagg ccgcggtcat cgccgtgccg catccgaaat
     1141 gggccgagcg cccgctggcg gtgatcgtgc tcaagcccgg ggcgagcgcc accgaggcgg
     1201 acatcaggag ccatctcgcg cagcgtttcg tgaagtggat ggtgcccgac gcctacgcgt
     1261 tcgtggacgc gatcccgcgc acctcgaccg ggaaattcct gaagaccagg ctgcgcgagc
     1321 aataccgcga ttggaagtgg gcggagcaga catgaacttc gagccgagcg acgagcagaa
     1381 gctgatcaag cacacggcgc gggagttcgc gcgcaaggag atcgcgccgc gcgcggccgc
     1441 ctgcgaccgc gaagcgcgct tccccatgga ggtgcagcag caggcgcgcg atatcgggct
     1501 ggtgaacatg accgtgccgc gcgagcacgg cggcagcggc tggggggtgc tggaccaggc
     1561 gctggtcacc gccgagctgg cctggggctg caccggcatc accggcgcgc tcgggctcaa
     1621 tgccattttc ggcgacgtgt tccaggtggc cggcagcgtc gagcagcgga agcgcgcctt
     1681 cggccggctg ctcgccggcg agttcggcgc ctacgccgtc accgagccgg ccgcgggctc
     1741 ggacgtcgcc cgcatcaaga cgcgcgcggt gcgcaaggcc gaccgctacg tgttgaacgg
     1801 cagcaaggtg tggatctcga acgccccgct cgccagcatg ttcgtggcgt tcgccaagac
     1861 cgaccccgaa gccgggcaca aggggatcag cgcgtttttc gtggaccggg acacgccggg
     1921 cctgagggtc gggccgccgc tcggcaagct cggccagaag gcgagcccgg cggccgaggt
     1981 gttcttggag gacgtggagg tgccggtctc ggcgttgatc gacgccgaag gcgccggttt
     2041 cctgatcgcg atgcgcgtgt tcgaccgctc gcggccgatg atcgcggcgc tggcactggg
     2101 tctgatgcag cgctgcctgg acgaagcgct gcagtacgcg cgcacgcggc agacgatggg
     2161 tcagccgatc atcgaacatc aggcgatcgg ccacaagctg gccgacatgg cgctacgcac
     2221 cgaggccgca cgcctgctca ccgagcaggc cgcctggatg ctggacacgg ggcggcgcaa
     2281 cacgctgcag gcggcctacg ccaagacctt cgccgcggac agcgcgatgt gggccgccac
     2341 cgaggcggtg cagatcttcg gcggcatggg ctacagcacc gagtacccgg tggagaagct
     2401 gttccgcgac gccaaggtgc tgcagatcta tgaaggcacc agcgagatcc agcgcaacat
     2461 catcgcccgc gagctcgggc ggcagtaacc ggtcaggagg ccgaagatgg ctgaagcagt
     2521 gatcgtggat gcagtgcgca cgccgttcgg ccggcgcggg ggggcgctgc gcgagcagcg
     2581 tccggacgcg atgctggccc acgccctgaa ggcggtggtc cggcgtgcgg gtgtggatgc
     2641 cacgcgcatc gaggacgtgg tggacggcac ggtgacgctc gccggcgagc agggcgcgaa
     2701 cccgggccgg ctcggcgtgc tgctggccgg cttcccggtc gaggtgcccg gcgtctcgct
     2761 gaaccgcatg tgcggctcga gccagcaggc gctgcacttc gccgcacagg cggtcgcggc
     2821 gggggacatg aactacgtca tcgcttcggg ggtggagagc atgacgcgcg tgccgatgtt
     2881 cctcgacatc agcctcggac gcgagggcgt gcacggcttc gaagtgttga accccgagct
     2941 gttccgccgg cacgagctga tccaccaggg ggagagcgcc gagcgcatcg ccgagaagtg
     3001 gggcctgtcg cgccaggcgg tggacgagtt cgccaaggag agccaccggc gcgccgcccg
     3061 ggcacagcaa gcgggctgga accgcgagat cgtgccgatc gagggcgtga gccccgaggc
     3121 gcaaaagatc gaagtcgtgc gcgacgaggg cgtgcgcgcg gtgcttgacg agcagaagat
     3181 cgccgccctg aaacccgcgt tccggcccga aggcaagggt gtggtcaccg ccgccaattc
     3241 cagccagatc tccgacggcg cgtcggccgt gctggtggcc aacgccgatg cggcgaaagc
     3301 ggacggggtc aaggcgaggg cgcgcttccg cgcgcgcgtg gtggtcggct ccgatccgac
     3361 gatgcagctc accggcgtca tccccgccac gcgcaaggcg ctgcagcgcg ccgggctgtc
     3421 ggtgcgggac atcgactggt tcgagatcaa cgaggccttc gccgtggtgc cgctgtgctg
     3481 ggcgcaggac ctgggcatcc cgctggagaa ggtgaatccc tggggcggcg cgatcgcgca
     3541 cggccatccg ctgggcgcca ccggcggcgg cctgatggcg aagatgctcg cgggcctcga
     3601 ggccaccggc ggccggttgg ggctgcaggt gatgtgcatc ggccacggcc aggccaccgc
     3661 gacgatcgtc gagcgagtgt aaaccggcac gcccgccctc caaggggcgg gaacgggttc
     3721 ttaaatcgcg cctttagggc gcacccggcc cggctcttgc ctgcgtcagc ggcgtctcga
     3781 ccccgcaggc ggcgcatgtt ccgctctttc cgcatcctgg ttctgctcct ggtgttgttc
     3841 actgtggccc tgggcaactg gctggcgcgc gcgcgcagca cctcgtggga gcactcgctg
     3901 cgcatggtgg tgtttccgat cgacgccgac ggcagccccg cgaccgcgcg ctacatcgcg
     3961 tcgctcagcc gcgagacctt cgcgccgatc gacgccttca tggcgtccga ggcgcagcgc
     4021 tacggactgg cgctgcgcga tccgctggac gtcgacgtgg caccgccggt gacccggctg
     4081 ccgccggccg tcccgcacgg cgggaatgcg ctgcagatcg tgctgtggag cctgcggctg
     4141 cgctactggg cgtgggtcaa cgcggagtac cctggaccta cgccgcaggt gcgcatgttc
     4201 gtgctgtatc acgatcccga ccgcgtgagc cgcctgaatc attcgctcgg actgcagaag
     4261 gggttgatcg gcgtggtcaa cgccttcgcc gcggatgccc aggcggggcc caacaacgtg
     4321 atcatcgcgc acgagatgct gcacaccttg ggcgccaccg acaaatacga tccgttgacc
     4381 aactatccgt tgcacccgat cggctttgcc gaaccggagc tcgcccccgt cgtgccgcag
     4441 cgcttcgccg aaatcatggg cgggcgcgtg ccgatgtcgc gggccgacgc gcgcattccc
     4501 gacgatctgg acgaggtcct gatcggcgcg gccaccgcgc tggaaatccg ctggctgcag
     4561 tgatccagcc tggcccggcc ggcgttttgg ggtagattcg ctcccatggc caccgaccac
     4621 cccaagggtt tgcgccgcaa cctcaccagt tacggcgacc gcgatttcgc gctctacctg
     4681 cgccgctcct tcgcccgcgc catgggctat tcgctggacc tgctcgaccg gccgatcgtc
     4741 ggcatcgcgc agaccggcag cggcttcaac aactgtcacc gcaccatgcc cgagctggtc
     4801 gaggcggtga agcgcggcgt gctcgccggc ggcgcgctgc cgctcgactt tcctaccgtc
     4861 tccctgggcg agatcttcct ctcgcccacc agcctgatgt tccgcaacct gatgtcgatg
     4921 gacaccgaag agatgatccg ggcgcagccg atggacgcgg cggtgctggt gggcggctgc
     4981 gacaagacgg tgccggcgca gctcatgggc gccgcctcgg ccgacctgcc cgcggtgcag
     5041 ctggtgaccg ggccgatgat ggcgatgcct tggcacggcg agcggctggg cgcgtgcacc
     5101 gactgccgcc gcttctgggc gcgccatcgc gccggcgaga tcgacgccgc cggcatcgcc
     5161 gaggtcgaag gcaatctcgc caccaccgcg ggaacctgcg cggtgatggg caccgccagc
     5221 accatggcct gcatcgccga ggcgctgggc atggctctgc gcgacagcgc cgcgatt
//
LOCUS       DASPGE010000125         5206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1090539, whole genome shotgun sequence.
ACCESSION   DASPGE010000125 DASPGE010000000
VERSION     DASPGE010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090539"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1852)
                     /locus_tag="VD791_03940"
     CDS             complement(212..1852)
                     /locus_tag="VD791_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HXV07155.1"
                     /translation="MHDLSLIVTLAGALAVALAFGWVTQRLGLSSLVGYMLAGIAVGP
                     HTPGFVADAQLASQLAEIGVILLMFGVGMHFHPGELLRVWRIAVPGAVGQSAVAALAG
                     WGAARAFGWSDAAGIVFGMALAVASTVVLMRMLVEQGRLSTRDGHVAVGWLIVEDLFT
                     VAALVVLPALALERSEPAGTAAALGLAVVKAGVFAVLIWAVGTRLVGSFLERIARTRS
                     SELFTLTVFVVALGVAVLAAGVFQVSVALGAFIAGLVLGRSRFGPQAASDMMPFRDVF
                     TALFFVSVGMLFDPRFALAQPLMVLAAVAIVLIAKPLVALAIVLALRDTRRTALTVAV
                     GLAQIGEFSFILATLGRSLGILPAEAVEVLVAAAIVSIAVNPLLFRGLRTLEARFAAA
                     GPGPPPQRPQETAVPAVETASAVAVAGLGDLGRHLARRCAASGIPVCVIDKDVEALEE
                     LRNSGILSVFGDPGRIDVLRAAAVKEAKIIVLTGMPLADKMRVCTAARALNPRIAIIA
                     TASSGAERAWLQEFGAAYVHDLLDEATEALLSALRRSL"
     gene            complement(2145..3041)
                     /locus_tag="VD791_03945"
     CDS             complement(2145..3041)
                     /locus_tag="VD791_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXV07156.1"
                     /translation="MLLRTSIGAALLASLALPAAALDGFAVYQQARPNMLQIVGVSPE
                     GRFHMGSGVALPNGTVVTNCHVTLRAKRVQLFWGGGAPSATLEAATVGHDLCALYFPE
                     LNRSGAAIGASRELQVGDPVFAIGYNAGHGLTYQPGEVAELYEHDGGMVVRTTAAFTH
                     GASGGGLFDQHGRLVAILTFFRVSKDKTDYFAVPVEWLKDLEGAPATPVAPLEGVPFW
                     ADEIGRQPAFLKAGALEADGRWEELATMARNWTETAPSDAHAWMALGKAATQLGDSVV
                     ADDAFRRAAELGATYPATMQSR"
     gene            3433..4818
                     /locus_tag="VD791_03950"
     CDS             3433..4818
                     /locus_tag="VD791_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07157.1"
                     /translation="MRTRSLVALLMLGLLPACDRWFGRDEDAVRQAYQSYREAMMTSN
                     LAKLKTLVARDKARELEAPDAAEMLQMASALYPEGTQIASLRLSGGEAALQLTGRMQG
                     GTAQGSVRLVKEEGAWKVSQEDWQVQISMGSEPGPAVAQEAQLPSNAVRPADYAALLG
                     RWQGGEGGVSDWTLTFGEGYAVTGQHASGAHYSGQAAIFWDLGATSDGIRVPPGWGVL
                     DVQVTQASEPRHVGEVSLGTFSKQGDMLKFCGSEPGTHVRTPSFEAPPSGVRCLTLAR
                     VGDAEAAPAPAAPATAPAPAPVVSRQESDGATGQAEVVLDGVPQSYPLFGGFVSDTRF
                     ADPRRATIQFKPPEWGSVSGLRLTLDATVTGRHYADGKAIQDMMFNGAEVQVGQDNGR
                     GRAAVLHWVADGGQVFPPKEGCAIEILSPYTGQPDSVFEGRISGCTVHSGGIDRQLAS
                     VKFAMHGAPSR"
     gene            4902..>5206
                     /locus_tag="VD791_03955"
     CDS             4902..>5206
                     /locus_tag="VD791_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07158.1"
                     /translation="MSRISRVEVHVFQFDAHNLGAVRGAGVGAFGYAKGAKTRISKYA
                     VAIMTDNGLRGEYVTHWVGSSAALGQTLMLAPYLIGRQAEEREQIYDDLKRQARQFD"
BASE COUNT          843 a         1797 c         1840 g          726 t
ORIGIN      
        1 cagctggtcc acatcgccgt agctgagagt ggtgcccagg ttggaatagg caggcaggtc
       61 gcggaaccgc tcgcaggagc gttccaggat gtctttgatc gacgagaatt cgtggatgtc
      121 cacctccggc ggcatgccgg gtggatagct tctcagccag ggcttttcca tgggttctcc
      181 ttcgaggcga tcgcagcgcc gtggcgcggg cctacaggct gcgccgcagc gcgctcagca
      241 gagcctcggt cgcttcgtcc agcaggtcgt gcacgtaggc ggcgccaaac tcctgcagcc
      301 acgcgcgttc cgcgccgctg gacgcggtgg cgatgatcgc gatgcgcgga ttgagcgcgc
      361 gtgccgccgt gcagacgcgc atcttgtcgg cgagcggcat gccggtgagc acgatgatct
      421 tcgcctcctt gaccgcggct gcgcgcagca cgtcgatgcg gcccgggtcg ccgaaaacgc
      481 tcaggatacc gctgttgcgc agctcttcca gcgcttccac gtccttgtct atcacgcaga
      541 ccggaatgcc gctcgcggcg cagcgccttg ccaggtgccg cccgaggtcg ccgagaccgg
      601 ccacggcgac cgccgatgcg gtttccaccg ccggcacagc agtctcctgc ggacgctgcg
      661 gcggtgggcc gggtccggcc gcagcgaagc gcgcctccaa ggttctcagg ccgcgaaaca
      721 gcagcgggtt gacggcgatc gagacgatgg cggcggccac cagcacctcc accgcctccg
      781 cgggcaggat gccgagcgat ctcccgagag tggccaggat gaaggagaac tcgccgatct
      841 gcgccagccc gaccgccacg gtgagcgcag tgcggcgcgt atccctgagc gccaggacga
      901 tcgccagcgc gaccagtggt tttgcgatca gcacgatggc gacagcggcg agcaccatca
      961 gcggttgggc gagcgcgaac ctcggatcga acagcatgcc caccgagacg aagaacagcg
     1021 cggtgaacac gtcgcgaaac ggcatcatgt cggatgccgc ctgtggaccg aagcgcgacc
     1081 ggcccaggac gagaccggcg atgaacgcgc ccagcgcgac ggagacctgg aacacgccgg
     1141 cggcgagcac ggcgacgccc agcgccacca cgaacaccgt cagcgtgaac agctcgctcg
     1201 agcgcgtgcg ggcgatgcgc tcgaggaacg aacccaccag gcgcgtgccg actgcccaga
     1261 tcaacacggc gaagactccg gccttcacca ccgcgaggcc gagcgcggct gcggtcccgg
     1321 cgggttcgct gcgctccagc gccagcgccg gaagcaccac caacgccgcc acggtgaaca
     1381 ggtcctcgac gatcagccag ccgacggcga cgtgaccgtc gcgtgtgctc aaccgtccct
     1441 gctcgacgag catgcgcatc agcaccacgg tgctggccac ggccagtgcc atgccgaaca
     1501 cgatccccgc agcatcgctc cagccgaagg cgcgcgcggc gccccagccg gccaatgcgg
     1561 ccacggcgct ctgtccgacg gcgccaggca ctgcgatgcg ccacacgcgc agcagctccc
     1621 cggggtgaaa gtgcatgccg acgccgaaca tcaacaggat cacaccgatc tcggccagct
     1681 gcgacgccag ttgcgcatcg gcgacgaacc cgggggtgtg cggcccgact gcgatgcccg
     1741 ccagcatgta gcccaccagg ctggagaggc ccaggcgctg cgttacccag ccgaaggcca
     1801 gcgcaacggc cagcgcgccg gccagggtca cgatcagaga caggtcgtgc atggaaagtc
     1861 agggagcggc atcccgatat cgcgccggtc gcactcgaat tgcaggtcgg cgaggattgt
     1921 cgcaagcgtc gcctgtcgcg cgggctacgt caaccgcgtg acgggagtgg caaaaagaac
     1981 tgccccggcg ccgtcggcac gacgatccgc cgggaatcgg gtcgcttcta cctgcccggc
     2041 agcgacgcag cgcccggtca gctgtagccg aaccgcggcc ggccgcgaaa aaaacaggca
     2101 gcgggtgctc cgctgcctgc ggggaagcgg ctgcaggcga tgcgtcagcg cgactgcatc
     2161 gtcgccggat aggttgcgcc gagctctgcg gcgcgccgga acgcgtcgtc ggccacgacg
     2221 ctgtcgccga gctgcgtagc cgccttgccg agcgccatcc aggcgtgcgc atccgagggc
     2281 gcggtttccg tccagttgcg ggccatcgtc gccagctcct cccagcggcc gtcggcctcc
     2341 agcgcgccgg ctttcaggaa cgcgggctgg cggccgatct cgtcggccca gaatggcacg
     2401 ccctcgagcg gagcgaccgg cgtcgcgggg gcgccttcga ggtccttcag ccactccacc
     2461 ggcacggcga agtagtcggt cttgtccttc gacacgcgga agaaggtgag gatcgccacc
     2521 aggcgcccat gctggtcgaa caggccgccg ccggaggcgc cgtgcgtgaa ggcggcggtg
     2581 gtgcgcacca ccatgccgcc gtcgtgctcg tacagctcgg ccacctcgcc cggctgatag
     2641 gtcaggccgt ggcccgcgtt gtatccgatc gcgaacaccg ggtcgccgac ttgcagctcg
     2701 cggctggcgc cgatcgcagc gccgctgcgg ttgagttccg ggaagtacag cgcgcacaga
     2761 tcgtggccga cggtggccgc ttcgagcgtc gcgctgggcg ccccgccgcc ccagaacagc
     2821 tgcacgcgct tggcgcgcag cgtcacgtga cagttggtga cgacggtgcc gttgggcaga
     2881 gccacgcccg agcccatgtg gaagcggccc tcgggactga ctccgacgat ctgcagcatg
     2941 ttcgggcgcg cctgctgata caccgcgaac ccgtcgaggg ccgcggcggg caaggcgagg
     3001 ctcgcgagca acgctgcgcc gatcgatgtc ctgagcaaca tggatactct cctgctgtga
     3061 tcgagaatat cctcccccac gcacccgcgc tcgtgcccgc gcatgcaccg ccgcgggcgg
     3121 tgatgcccca ctgcggtgcg ctacggccgg cagccggtgc taggggcgta gcttgcttcg
     3181 gattcatcgc gatttcttgt ctgcggctcg tgccggcgac ggcgacaggc agacgccctt
     3241 gtcacgcact acggcaagat gcatgccttc tgaagggccg agtccgtcaa aggttccccg
     3301 ccacgcgaca tgccgcctgg ccgatggagg cgcgcaggag cgggcgcgtg cgctgcgggg
     3361 ccaacggggg gtgaatctcc gtgtagcgca gccgatattc agaccgtccc agggccccga
     3421 aggaggtcca agatgcgaac ccgatcgctg gttgcgctgc tcatgctcgg cctgttgccc
     3481 gcttgcgacc ggtggttcgg ccgcgacgag gacgccgtgc ggcaggccta ccagagctac
     3541 cgcgaggcga tgatgaccag caatctcgcc aagctgaaga cgctggtcgc acgcgacaag
     3601 gcgcgagagc tggaagcgcc cgatgccgcg gagatgctgc agatggcgag cgccctgtac
     3661 ccggagggta cccagatcgc gagcctccgg ctgagcggtg gcgaagcggc gctgcagctc
     3721 accgggcgca tgcaaggcgg taccgcccag gggagcgtgc gcctggtgaa ggaagagggc
     3781 gcctggaagg tgagccagga ggattggcag gtccagatca gcatgggcag cgagcccggc
     3841 ccggctgtcg cgcaggaggc gcagctgccg tccaacgcgg tgcggccggc ggactacgcg
     3901 gccctgctcg gccggtggca gggcggagag ggcggggtga gcgactggac gctgaccttc
     3961 ggcgagggct acgcggtgac cgggcagcat gcctccgggg cccactacag cgggcaggcg
     4021 gcgatcttct gggacctcgg agcgacctcg gacggtattc gcgtgccgcc cggctggggc
     4081 gtgctcgacg ttcaggtcac tcaggcctcc gagccgcggc acgtcggcga agtgtcgctc
     4141 ggcactttca gcaagcaggg cgatatgctg aagttctgcg gcagcgagcc gggcacgcac
     4201 gtgcgcaccc cgtccttcga agcgccgccg tcgggtgttc gctgcttgac gctggcgcgc
     4261 gtcggcgacg cggaggcggc gcctgcgccg gccgcaccgg caaccgcccc ggcgcctgca
     4321 cccgtcgtgt cgcgccagga gagcgatggc gccaccggtc aggcggaggt ggtgctcgac
     4381 ggcgtgccgc agagctaccc gctgttcggt ggattcgtct ccgacacccg ctttgcggac
     4441 ccgcgccgtg ccacgatcca gttcaagccg ccggaatggg gcagcgtctc cggattgcgc
     4501 ctgaccctgg atgccacggt gaccggtcgc cactatgcgg acggcaaggc gatccaggac
     4561 atgatgttca atggcgccga ggtgcaggtc gggcaggaca acggccgcgg ccgcgccgcg
     4621 gtgctgcatt gggtcgccga cggtggacag gtgttcccgc ccaaggaggg ctgcgcgatc
     4681 gagatcctgt ctccctacac cgggcagccg gacagcgtgt tcgagggccg catctccggc
     4741 tgcaccgtgc attccggcgg catcgaccgc cagctcgcct cggtgaagtt cgcgatgcac
     4801 ggcgcgccct cgcgctagcc gccgcggcgc ggctctcctt gcggcggcgg ccccgttgcc
     4861 ccggtgctat cctttcccgc tcgctcgggg ggaggagccg catgagccgc atcagcaggg
     4921 tcgaagtcca cgtcttccag ttcgacgcgc acaacctggg cgcggtgcgc ggcgccggtg
     4981 tcggcgcgtt cggctacgcc aagggcgcca aaacgcgcat cagcaaatac gccgtggcga
     5041 tcatgaccga caacggcttg cgcggagagt acgtcacgca ctgggtcggg tcctccgcgg
     5101 cgctcggcca gaccctgatg ctcgcgccct atctgatcgg gcgccaggcg gaggagcgcg
     5161 agcagatcta cgacgatttg aagcgccagg cccggcagtt cgacca
//
LOCUS       DASPGE010000126         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1090719, whole genome shotgun sequence.
ACCESSION   DASPGE010000126 DASPGE010000000
VERSION     DASPGE010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090719"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..743
                     /locus_tag="VD791_03960"
     CDS             60..743
                     /locus_tag="VD791_03960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV07159.1"
                     /translation="MELTIGPLTPDLWAGLEDLFGEHGGWRMYWRIGSSYRKRPRQAN
                     KAAFWEVVERGPPPGLLALDGAVAVGWCQLTPREALPWLDRTWRLKRMDDLPVWSLSC
                     LCVRNSRRRRGVTSALIAAALRAAKRGARLRSKPIRSMRTCRRALRAPATPRPSPAPA
                     SAPSPAARPPARSCATISWRLPDLRHGQRGGAAAAPSMMLNLTGLLRHPVAGACGPVR
                     RTSEGVVPG"
     gene            1004..2329
                     /gene="glcF"
                     /locus_tag="VD791_03965"
     CDS             1004..2329
                     /gene="glcF"
                     /locus_tag="VD791_03965"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF008434.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HXV07160.1"
                     /translation="MQTTFSLAQLADPDVRESEKILRACVHCGFCTATCPTYLLLGDE
                     LDSPRGRIYLIKDMLENAKPASARVVTHIDRCLSCLSCMTTCPSGVHYMHLVDHARRY
                     IEQTYTRPFSDRLLRRMLALALPRPGLFRLALRGAGLARAVRGLMPGRLAGMLALAPA
                     RLPSASPVDRPQRFPAEGVRRARVALLSGCAQQVLAPEINEATIRLLRRHGVEVVIAE
                     GGGCCGALAHHMGREAQALASAKANIDAWERTRTDGGLDAVIINASGCGTTVKDYGFM
                     LREDPAYAAKAAHIAALAKDITEFLHELGLQAPVPGRDLVVAYHSACSMQHGQRITAE
                     PKDLLCAAGFTVRDIPEGHICCGSAGTYNLLQPELATRLRERKLANIARVRPDVVATG
                     NIGCMVQLADSTTPVVHTVELLDWATGGPVPRALAAAAPPVPAAVAAAL"
     gene            2382..>2663
                     /locus_tag="VD791_03970"
     CDS             2382..>2663
                     /locus_tag="VD791_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HXV07161.1"
                     /translation="MLRVLHFPLGPFSRKVRIVLREKELDAELEAVEPWRIGDDFAAL
                     NPAAEVPVLLDGPRVICDSWAICEYLEETRPERRLLALDPVERAEVRRLV"
BASE COUNT          356 a          966 c          929 g          412 t
ORIGIN      
        1 cccgattggc gcggtatcat tggtcaagat cggagggatg cttggtccgc gacggcgaca
       61 tggagctgac gatcggcccg ctcacgcccg atctgtgggc cgggctcgaa gacctgttcg
      121 gcgagcacgg cggctggcgc atgtactggc ggatcggcag ctcctatcgc aagcgcccgc
      181 gccaggcgaa caaggcggcg ttttgggagg tcgtggagcg cggcccgccc cctggcctgc
      241 ttgcgctcga cggcgccgtc gccgtgggct ggtgccagct cacgccgcgc gaggcattgc
      301 cttggctcga ccgcacatgg cggctgaagc gcatggatga ccttccggtc tggtcgctct
      361 cctgcctctg tgtgcgcaac agccgtcgcc ggcgcggcgt cacgtccgca ctgatcgccg
      421 cagcgctgcg agccgcgaag cgcggggcgc gcctgcgctc gaagcctatc cgttcgatgc
      481 ggacgtgtcg ccgagcgctt cgggcaccgg ctacgcctcg accttcgccc gcgccggctt
      541 ccgcaccgtc gcccgccgca cgcccgcccg cccgatcatg cgccacgatc tcctggcgat
      601 tgcctgatct ccggcatggc cagaggggtg gcgcggcggc tgccccatcc atgatgctga
      661 acttgactgg cctgctccga catccggttg cgggagcatg cggtccagtg cggcggacca
      721 gcgaaggcgt tgtgccgggg tgaccggcgc ccggcgcgat cagcgtcccc gcgttgcagt
      781 cgttcaactc gaccccggcc atcatcgacc gcttggcggc agtgatcggc gcgcgcacca
      841 cggcgctggt cagggtcggc gggggtcgcc atatgtgggg ccgcgggtag ccgccggcgc
      901 ggcgcatcga tcgagttttt tgcccggatg acatcccgcc ggggcctcgt gatcgaggcg
      961 cgggcgtgat agggagagcc cacgcagggc cggactaggt cggatgcaga ccaccttctc
     1021 gctcgcccag ttggccgatc ccgacgtgcg ggaatcggaa aagatcctgc gcgcctgcgt
     1081 gcactgcggc ttctgcaccg cgacctgccc gacctacctc ctgctcggcg acgagctcga
     1141 cagcccgcgc ggccgcatct acctgatcaa ggacatgctg gagaacgcga agcccgcgtc
     1201 ggcgcgcgtg gtcacccaca tcgaccgctg cttgtcctgc ctgtcctgca tgaccacctg
     1261 cccgtccggc gtgcattaca tgcacctggt cgaccacgcc aggcggtaca tcgagcagac
     1321 ctatacccgg cctttttcgg atcgcctgct gcgccgcatg ctcgccctgg cgctgccgcg
     1381 cccggggctg ttccgcctcg cgctccgtgg cgccgggctc gcgcgcgcgg tccgcggcct
     1441 catgcccggg cggctcgccg ggatgctggc gctcgccccg gcgcgcctgc cgtccgcgtc
     1501 cccggtcgac cggccgcaga ggttcccggc cgaaggcgtg cgccgcgcgc gggtcgcgct
     1561 gctctccggc tgcgcgcagc aggtgctggc cccggagatc aacgaggcga ccatccgcct
     1621 tctgcggcgc cacggcgtcg aggtggtgat cgccgagggt gggggctgct gcggcgcgct
     1681 ggcccatcac atggggcggg aggcgcaggc gctcgcctcg gccaaagcca acatcgacgc
     1741 ctgggagcgc acgcgcaccg acggcggcct cgacgccgtg atcatcaacg cctcgggctg
     1801 cggcaccacg gtcaaggact acggcttcat gctgcgcgag gacccggcat acgccgcaaa
     1861 ggcggcgcac atcgccgcgc tggccaagga catcactgag ttcctgcacg agctcgggct
     1921 gcaggcaccg gtgcccggga gggacctcgt ggtcgcctac cattccgcct gttcgatgca
     1981 gcacggccag cgcattaccg cggagcccaa ggatctgctc tgcgccgccg gcttcaccgt
     2041 gcgggacatc cccgaaggcc acatctgctg cggctccgcc ggcacctaca acctgctgca
     2101 gccagagctc gccacccggc tacgcgagcg caaactggcc aacatcgcgc gcgtacgccc
     2161 ggacgtggtc gcgaccggca acatcggctg catggtgcag ctggcggaca gcaccacgcc
     2221 ggtggtgcac accgtggagc tgctggactg ggcgacgggc gggccggtgc cgcgggcgct
     2281 cgcggccgcc gccccgccgg ttcctgccgc ggttgcggcc gccctctgaa tccgcacata
     2341 aactcttgcg cccaacctcc gatttttgct aagcaatacc catgctgcgc gtgctgcact
     2401 ttccgctcgg cccgttctcg cgcaaggtgc gcatcgtgct ccgcgagaaa gagctggatg
     2461 cggagctgga agcggtggag ccctggcgga tcggcgacga cttcgccgcg ctcaacccgg
     2521 cggccgaggt gccggtgctg ctcgacgggc cgcgcgtgat ctgcgacagc tgggcgatct
     2581 gcgagtacct ggaggagacc cggccggagc ggcggctgct ggcgctcgat ccggtcgagc
     2641 gcgccgaggt ccggcgtctg gtg
//
LOCUS       DASPGE010000127         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1325081, whole genome shotgun sequence.
ACCESSION   DASPGE010000127 DASPGE010000000
VERSION     DASPGE010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325081"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VD791_03975"
     CDS             complement(<1..353)
                     /locus_tag="VD791_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HXV07162.1"
                     /translation="MAQAPATALTRPTLRQMAAPLLILMILAMMVLPLPPIALDLLFS
                     FNIALSMLVLLLALYVLKPLDFSLFPTVLLLTTLLRLSLNVASTRVVLLHGHTGPDAA
                     GKVIEAFGHFLVGGN"
     gene            complement(645..1808)
                     /locus_tag="VD791_03980"
     CDS             complement(645..1808)
                     /locus_tag="VD791_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HXV07163.1"
                     /translation="MAEESDLERTEPASQRRLEQARERGQVPRSPELSSFAVLMAAGG
                     GFLWMGAALVDALEGVMRAGLTLDHAATFDAARLGARLYDGAAAALLGFAPWFLLVAA
                     VGILAPTLMSGWLFTFQPLQPDLSRLDPARGLGRIFSRQGVIESGKALLKIAAVGAAA
                     AWALWAERAEIATLLSEPLQPGLAHLGHLLGATFLIVAGALALIVALDLPLRLWDHGR
                     QLRMSRDEVRAELKETEGDPLVKARIRALQREMTRRRMMLEEVPRADAVVTDPGRCAV
                     ALRYAEDTMRTPRVVARGALRVAERIVDLARANDVPVLSAPALARALLAHAAVGRDIP
                     APLYDAVADVLAWVYQLRRFRTAGGERPREPGALPVPPELDPGRFSDAEETSR"
     gene            2550..>3056
                     /locus_tag="VD791_03985"
     CDS             2550..>3056
                     /locus_tag="VD791_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV07164.1"
                     /translation="MRIPTKLLSASLLLAFTAPGAALANEGLAKLMADSKNWAAQAGD
                     FANTRYSALKQVNAGNVNRLQVAWMFSTGVLRGHEGSPLVVGDTMYLHTPFPNNVFAI
                     DLETQKIKWKYAPKQDPAVIPQMCCDTVNRGLAYADGKLFLQQADSKLVALNAADGRV
                     VWTAVNGDP"
BASE COUNT          534 a         1054 c         1026 g          442 t
ORIGIN      
        1 aagttgccgc cgaccaggaa gtggccgaag gcctcgatta ccttgcccgc ggcatccggc
       61 ccggtgtgac catgcagcag cacgacgcgg gtgctggcca cgttcaacga gaggcgcagc
      121 agcgtggtga gcagcagcac cgtcgggaac aacgagaagt cgagcggctt gaggacgtac
      181 agggcgagca gcagcaccag catcgacagc gcgatgttga agctgaacag cagatccagc
      241 gcgatgggcg gcagcggcag caccatcatc gccaggatca tgaggatcag caacggcgcg
      301 gccatctgcc gcagagtcgg gcgcgtcaac gccgtcgccg gcgcctgggc cattaccgcg
      361 acacctcaac ccaaaggtgc agaggacgca gagaacgcag ggaggggcgc ttctccgcag
      421 cgccacaccc gccgacgggc gggcatgcaa tggcgtcacc gcgggatggg ccgggaggga
      481 ggaggaggag tgcccggggt ccatccgtca ggtggcgctc tgcgccctct gcgttctctg
      541 cacctctggg ttgggggttc atcttgctca aagccattca ccgcggtgga cgcggaggaa
      601 aaacaacatc gcgtttcctc tgcgtcctcg gcgtcctctg cggttcatct tgaggtttct
      661 tccgcatccg agaaccgacc gggatcgagc tcgggcggga ccggcagcgc cccgggctcg
      721 cgcgggcgtt caccgccggc ggtgcggaag cggcgcagct ggtaaaccca cgccagcacg
      781 tcggctaccg cgtcgtacag cggcgccggg atgtcccggc cgactgcggc gtgcgcgagc
      841 agcgcgcgag ccagcgccgg cgcgctcagc accggcacgt cgttggcgcg cgccaagtcg
      901 acgatccgct ccgccacccg cagcgcgccc cgggccacga cccgcggcgt gcgcatggtg
      961 tcttcggcgt atctcagggc gaccgcgcag cggcccgggt cggtcaccac cgcgtcggcg
     1021 cggggcacct cctcgagcat catgcggcgc cgtgtcatct cgcgctgcag ggcgcggatc
     1081 cgcgccttca ccagcgggtc gccttcggtt tccttgagct ccgcgcgcac ctcgtcccgg
     1141 ctcatgcgca gctggcgccc gtgatcccac aaccggagcg gcaagtccag cgccacgatc
     1201 agcgcgagcg cgccggccac gatcaggaag gtcgcgccga gcagatgacc gaggtgcgca
     1261 aggcccggct gcagcggctc ggacagcagc gtcgcgatct cggcgcgctc ggcccacagc
     1321 gcccacgccg cggctgcgcc gactgcggcg atcttcagca gcgctttccc cgattcgatc
     1381 acaccctggc gcgagaagat gcggcccagg ccgcgggccg gatcgagacg cgagagatcc
     1441 ggctgcaggg gctggaaggt gaacagccag ccgctcatca gcgtgggggc gagaatgccc
     1501 acggcggcca ccagcaggaa ccagggcgcg aagccgagca gcgcggccgc ggcgccgtcg
     1561 tagagccgcg ccccgagccg ggcggcatcg aaagtcgcgg catggtccaa agtgaggccg
     1621 gcgcgcatca cgccctcgag cgcgtccacc agcgccgctc ccatccacaa gaaaccgccg
     1681 ccggccgcca tcagcacggc gaagctggag agctcgggag agcgcggcac ctggccgcgc
     1741 tcgcgggcct gctccaggcg tcgctgtgac gccggttctg tgcgttcgag atcgctttcc
     1801 tcggccatcc tccgcctcgc cggcgtgtcc cctcacgggc cggaaccggc tggtctcgcc
     1861 gcgcgccacc gaccgggcgc gcgcgcaggc cggttcccgt gcagtctagc cgagttgcgg
     1921 accggaacac aagatatttg atacctgtcg cctggcgacc actatattta gtggttgagg
     1981 tccagtctac caaagctctg accgcaaagg cgcagaggaa ccgcagagga aagcgagtcc
     2041 tgcgggagcc cggcgaatcg gttgcctctc ccatcacgct gccgcggctc tctgagtctt
     2101 tcctttgcga ccctctgcgc ctctgcggtt gcctgaagca aaacctaaca aagcggaagg
     2161 ttcgaccgac cagcactctg cggaagcgct gctttgcctt gacggctgcc aagcgcggtg
     2221 ctattcattt cgtcgcatgt tcaccacgca acggcctcgc gcagaagggc cagcagaata
     2281 accataacgc tcgcggtagc atcccgccag ggtttccagc tgccgtttcg gcgtgcactg
     2341 ttgacgcacg ccagccagac aagaaagccg aacgactcgc gctcggcccg gactgcatgt
     2401 ggatgcgacg ggccgctggc agcatcacct tatcgatcgg cgccgtttgc acgcgccgca
     2461 acgggctggc ccaccgcatt tccagtgcgg acggtgccgg cttgcgaacc ttgcaccaag
     2521 aaccaatcga ctacaggcgg aggaccaaag tgaggattcc taccaagctg ctttcggcgt
     2581 cgcttctgct cgccttcacc gcgccgggcg ctgccctggc caacgagggg ctggcgaagc
     2641 tgatggccga ttccaagaac tgggcggcgc aggctggcga tttcgccaac acgcgctaca
     2701 gcgcgctcaa gcaggtcaac gcgggcaacg tcaaccggct gcaggtcgcg tggatgttct
     2761 ccaccggcgt gctgcgcggg cacgagggct cgccgctggt ggtgggcgac accatgtacc
     2821 tgcatacccc tttccccaac aacgtcttcg ccatcgactt ggagacacag aagatcaagt
     2881 ggaagtacgc gcccaagcag gacccggcgg tgatcccgca gatgtgctgc gacacggtca
     2941 atcgcgggct ggcgtacgcc gacggcaagc tgttcctgca gcaggccgac agcaagctgg
     3001 ttgcgctgaa cgcggcggac ggccgggtgg tgtggaccgc ggtcaacggc gatccc
//
LOCUS       DASPGE010000128        19712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1559937, whole genome shotgun sequence.
ACCESSION   DASPGE010000128 DASPGE010000000
VERSION     DASPGE010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19712
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559937"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..715
                     /gene="napH"
                     /locus_tag="VD791_03990"
                     /pseudo
     CDS             59..715
                     /gene="napH"
                     /locus_tag="VD791_03990"
                     /inference="COORDINATES: protein motif:HMM:NF007013.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
     gene            755..2356
                     /locus_tag="VD791_03995"
     CDS             755..2356
                     /locus_tag="VD791_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV07165.1"
                     /translation="MGTRFAKLSRIPATTLAVALAALCVVAGPVQAKTFVYANQGDTL
                     SMDPHMLNESLLLNFTGNVYESLVGRGRKLELEPELATDWKRTSATVWRFNLRKGVKF
                     HDGTPFTADDVIFSWQRARGEGSDMKTYVGQIAEIRKIDAHAIDIVTSEPFPILPDVL
                     TLWRIMSKSWCERNNAVRPVDVRRGTENHASNHANGTGPFMLQSRQPGVRTVLVANPG
                     WWGKPEHNVTEARFLPIANEATRVAALLSGEIDMMEPVPLQDVQRLRNDARLKVMQSP
                     ELRTIFLGMDQKRDALIYSSVKGRNPFKDRRVRQAFYQAIDIQAIRSRIMRGAATPTA
                     LMIAPGINGFDPALNQRLPYSPEAAKKLLAEAGYPNGFEVGMNCPNDRYVNDAEICQA
                     VAVMLARIGVKVNLMAETKATFFPKVLSRETSFYLLGWTPGTYDSHNPLFSVLSTPGE
                     GGQGQFNLGAYSNARVDELTRAIGSETDPVKRQAMIGEAFKLHAEDIGHLPLHQQMLT
                     WGMKKNIELTQRADNFNYLKWVVIR"
     gene            2357..3271
                     /locus_tag="VD791_04000"
     CDS             2357..3271
                     /locus_tag="VD791_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV07166.1"
                     /translation="MREAWLRAWDSDVAYSFRHSPVTMAAALATLLCVGAALFAPWVA
                     PHDPFDLASLDLLNAFTPPAWDARGQASYPLGTDDQGRDVLSTILYGARISLAVGVAS
                     VVFALVLGVGLGLAAGYAGGRIDALIMRLADVQLSFPAILIALLIDGLVRVALPAERH
                     ADIAVYVLVLAIGVSGWVQYARTVRGSTLVEKSKEYVQAARVIGRRPAAIMLQHVLPN
                     VLGPVLVIATIHLATAIITEATLSFLGVGVPPTTPSLGTLIRVGNDYLFSGEWWIAVF
                     PGIALVALVLSVNLLGDWLRDALNPRLR"
     gene            3268..4245
                     /locus_tag="VD791_04005"
     CDS             3268..4245
                     /locus_tag="VD791_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV07167.1"
                     /translation="MSPASPLLEVKRLRVEFQTRRGALLAVDDVSFAIGRGEVLGVVG
                     ESGAGKSLTGMAIIGLLEPPGRIAGGEVRLDGQRIDDLPQAELRRIRGRRIGAIFQDP
                     LTSLNPLYTVGQQLVETIRTHLPVSQREARARAIALLRETGIPAPEQRIDQYPHQFSG
                     GMRQRAVIALALCAGPELIVADEPTTALDVSIQAQIIGLLKRMCREHGAAIMLITHDM
                     GVIAETADRVAVMYAGRIAEIGPVREVIHAPRHPYSAGLMGSIPKPGQARATLVQIDG
                     AMPRLNAIPSGCAFHPRCPRQFARCTQQRPELIPAGSSRAACWLHADER"
     gene            4235..5293
                     /locus_tag="VD791_04010"
     CDS             4235..5293
                     /locus_tag="VD791_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HXV07168.1"
                     /translation="MSAEAAALLVLRDVGRDFDVSRPWLERALERTPRRVLKAVDGLS
                     LDIRRGETLALVGESGCGKSTVARMIVGLHAPSRGRIEFEGVELTAARPAPEVARARR
                     RMQMIFQDPYASLNPRWRVERIVAEPLRAQSHAAGRDAIRARVAELLLQVGLTPEDGG
                     KYPHEFSGGQRQRICIARALSAHPEFLVCDEPTSALDVSVQAQILNLMKALQQRLGLT
                     YLFISHNLAVVAYIAGRIGVMYLGRLVELADAATLFAQPQHPYTRMLLDAVPDLQMSG
                     RPRTPVAGEVPNPLDPPSGCAFHPRCPHADARCRAERPALRRAAAGSMVACHAVEEQR
                     LPAWTGRFSSTPQRAGVQ"
     gene            complement(5295..6467)
                     /locus_tag="VD791_04015"
     CDS             complement(5295..6467)
                     /locus_tag="VD791_04015"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXV07169.1"
                     /translation="MIRLSSLIRRAPPCTRAIALAALLSTHALPASGQAPRSPTPDAK
                     SFPGSVTVIARGLEHPWGLALLPEGGMLVTERSGQLRRIAADGKVSAPLAGVPEVYAR
                     GQGGLLDVALSPDFARDRLVYLSFSEPGEGGAGTAVARGRLGERGLEDTQVVWRQQPK
                     VAGANHWGSRLVFAPDGTLFVTLGDRAGYRQQAQNLEAGLGKVVRLNPDGSAPGDNPF
                     VQGGGRPDIWSYGHRNIQAAALHPETGQLWTVEHGARGGDELNQPQRGSNYGWPVITY
                     GVDYSGAKIGEGTAKAGMEQPVYYWDPVIAPSGAVFYTGTAFPAWHGNLLVGSLNPGG
                     LVRLVLRDGRVAAEERYRGRELSARIRDVAQGADGSVYLLTDSSDGRVLRLAPAGG"
     gene            complement(6551..7987)
                     /locus_tag="VD791_04020"
     CDS             complement(6551..7987)
                     /locus_tag="VD791_04020"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HXV07170.1"
                     /translation="MQNAVQTTGTRATSGAEEALATRIARTVAQLSTAQLSGEVADKI
                     PEAVIEKTKVCLMDLIGCAFEARDLPWSRQAVQLAAPVAEGAGAATVIGSPRPAAAGD
                     AAFANAVMGHGLVREDMHAGSISHLGIVVLPALLALAQSRPVAGRDFIAATVAGYEVG
                     CQIGRVLMDAAVARIFRPTGISGPLAAAAAGARLLGFDAGQTASALVLAANTTAGFNQ
                     WAHTGGSEMFFHAGFAARNALTAVRLAGLGAFASPTGLDGEAGLFAALGKREAAARVR
                     LFAGPPELLSVYHKPLPACNFAQTPSQAALRIARDSGCRPERIAAVVVRVSRAGAAYP
                     GCDYRGPFAHVLQAKMSIQYNVAAALIRGELSEDSFAALDDAQVRRLIGLTTLQVDDD
                     MTRAYPGKQGAEVEVREASGATHRVRLDDVVNATAAEVRTRLRGAAARVLGAARAGQI
                     EGFIDRLELSEDVGHLGTLLRGAPRTPA"
     gene            complement(8002..8454)
                     /locus_tag="VD791_04025"
     CDS             complement(8002..8454)
                     /locus_tag="VD791_04025"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HXV07171.1"
                     /translation="MEPLLKTFGKPFHLGEKAGQAQTMKLANNLLAASALAVTSEAMV
                     MGVKAGLDPKTMLDVINAGSGRNSASQDKFPRAVLPGTFDFGFATGLSYKDVRLCIEE
                     AEAMGVPMVAGAAVREMLAVTNALYGGDSDFTSICRVVESWAGVQVRG"
     gene            complement(8434..8898)
                     /locus_tag="VD791_04030"
                     /pseudo
     CDS             complement(8434..8898)
                     /locus_tag="VD791_04030"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
     gene            complement(9039..10136)
                     /locus_tag="VD791_04035"
     CDS             complement(9039..10136)
                     /locus_tag="VD791_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXV07172.1"
                     /translation="MKIEAVETIVLRIPYTCGGSTDTEAWGGKPWETADALLVKISTD
                     AGLTGWGEAFGYNAIPATRAAIEHMLAPMCVGRDPLQIESLTLALQQKLHIFGRGGPV
                     IFGLSGIDIALWDIVGKAAGQPVHRLLGGCRLPRLPCYASLIRYSDPKLVADNVARAL
                     SQGFRHVKLHEIAVAPTRAARDAGGQDVDIMLDVNCPWSLREALDMAEKLRPLHLRWL
                     EEPVWPPEDYAALAQVRARGGIPIAAGENAATLQQYRHMFEAGAVDFAQPSPIKMGGI
                     SELCKVFALAAAHNVTVVPHSFYDGPGLLAALHVNAALGQGSLVEWRYFDLEARLYGD
                     AAVPREGSIAVPLGPGLGLDPDPEVIKRYRQ"
     gene            complement(10207..11484)
                     /locus_tag="VD791_04040"
     CDS             complement(10207..11484)
                     /locus_tag="VD791_04040"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HXV07173.1"
                     /translation="MNLAVLSLIALFLAIVLGTFTQANVGILSIAGAFAIGVLIGGLP
                     LKDVAAGFPVNLFLTLVAITFLFAQARANGTLERVSSVAVRVAAGHPGYIPFAFFGLA
                     LAFATLGPGNIATTALLAPVAMAAAGRAGIPAFLMALMVCNGANAGALSPFAPTGIIA
                     NGLMARIGLEGHEWRNYFNALLPQTVVALAGYLALGGWRLMRQPRADAPGVVVDTTGG
                     AVQPASTPFDWRQKLTLAVIAALIVSVAVFKVDVTFGAFVAAVVLSLAGAAQERAAVA
                     AMPWATILMVCGVTVLIALVEKTGGMDLFTTLLAQFSTRETITGVIAFVTGVISVYSS
                     SSGVVLPAFLPTIPGLVDKLGGGDPLGIAASINVGAHLVDVSPLSTLGALCIAAAAPE
                     EDRKALFNRMLAWGLSMSVVGALVCWLFFGVLA"
     gene            11663..12634
                     /gene="dctP"
                     /locus_tag="VD791_04045"
     CDS             11663..12634
                     /gene="dctP"
                     /locus_tag="VD791_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXV07174.1"
                     /translation="MPRVPVVVLALLGTLSLCAPAARAQEVTLRALTSFSEGTFFSRN
                     FERLIEKINREGRGVLQIRYVGGPKAIPTMEAGNALRSGVVDIANIASAFYVNLVPEV
                     DAQKLARVPTAEQRRNGAFAYFNRLHNEKMNAVYLARQHGNIQFHLYLNKPIERSDLS
                     GLKIRVTPVYRDFFGALGATTVTTAPGEVYMALERGVVDGYGWPSVGIFDLAWHERTK
                     YRVEPAFYVVDVAVLVNLDKWNGLTAQQQKLLSDAALWLEGLDRENDALVAKELERQA
                     QAGIRPIRLEGAEAKRYLERAHQVGWDSIARISPAHAPKLRALLDKQ"
     gene            12631..13452
                     /locus_tag="VD791_04050"
     CDS             12631..13452
                     /locus_tag="VD791_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV07175.1"
                     /translation="MSETTTAMRLEGKIAIIVGAGQSPGEGIGNGRATALRFAQEGAR
                     ILAVDRDLASARETAAMVTAEGGECLAFEADVTKEQTLAAAVSFAQARWGRIDVLHNN
                     VGVSVGGGDAPLAELTEETFDRISAINLRGTIMACKHVLPVMRQQRDGAIVNISSVAA
                     WTEYPLVVYKATKAAMIAFTQQLAIQNAAYGIRANVILPGLMDTPMAVDTRARASRRS
                     RAEVAAERDAKVPLRQRMGSAWDVANAALFLASDEARFITGVALPVDGGALVRIG"
     gene            complement(13469..14737)
                     /locus_tag="VD791_04055"
     CDS             complement(13469..14737)
                     /locus_tag="VD791_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV07176.1"
                     /translation="MTGVIGPGHAPDAKLGVDSAYAWARLTAALLLSAIGGVGMWSVV
                     VVLPPVQAEFAAARSDASLPYTLTMIGYGLGGVLMGRLSDRFGILLPVIAGTIALAIG
                     YACAGLSAHLWQFTLAQGLLIGVGSSATFAPLLADTSMWFARCRGMAVAIFASGNYLA
                     GTVWPPVVQHYIESAGWRETYFGIALFCVATVLPLAAAVLRRRSPRDAPVVSKARPSS
                     LGAAPASPLSVGLSPGALQALLILAGLSCCIAMSMPQVHIVAYCGDLGYGAARGAQML
                     SLMLGFGIVSRLASGWIVDHIGGVRTLLLGSSLQMLALALFLGFDGMVSLYVISALFG
                     LFQGGIVPSYAIIVREYFPAQEAGARVGAVFMATLFGMALGGWMSGAIFDLTGSYDAA
                     FVNGLLWNLLNVAIAAWLLRQGRQRLVAAT"
     gene            14811..15806
                     /locus_tag="VD791_04060"
     CDS             14811..15806
                     /locus_tag="VD791_04060"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXV07177.1"
                     /translation="MHNLPAVLALLMAAGLAAIPDPAGAQQIRMRFGHDQPVGSMYDE
                     GHTMLRKLLQERAGERVRMDIFPAAQLGSEVAMIEGVRLGSLDGSVAHVANAATVVPE
                     LALFSVSYLFKDREHYERVLNDPRFRERMDRLVASKNLGLRLIGFYSAGVRMLYTRRG
                     PAYTPEELKGTKIRVMNNPVEARIWQALGTIPTPMNFGEVYQALQSGVLDAAENAPSV
                     IESNRHYEAAKTLILTEHQRSICLLLINEKKFSGLPPDLQKTLLEAAAQAAAYERKRD
                     LELNSEAIARMQARGARVVTPERAKFIARIVPIQDQVARELKMTDVLELIRGHAR"
     gene            15796..16284
                     /locus_tag="VD791_04065"
     CDS             15796..16284
                     /locus_tag="VD791_04065"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXV07178.1"
                     /translation="MRAERVFERLERWLLPLLASLLALITAGVFVQVVLRYGFAKSFL
                     WGEELALFAFIWCVYLGAAICTWRRSHFAFDLFAGRLSGRAAGAQQLAVDLCVLLVAA
                     VMLVQGAAFAELSVQRLSPALGITLLVPTLAIPLSGGLMVLAALAALHRDLRLILTGR
                     AG"
     gene            16284..17579
                     /locus_tag="VD791_04070"
     CDS             16284..17579
                     /locus_tag="VD791_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXV07179.1"
                     /translation="MHPLLLLVGSLTLLLIAGVPIGFALLLSSLLVIWVEELPTAVMV
                     QQMFNGINAFTLLALPFFFLAGNVMTGGGISERLVKLAMALVGHRRGGLAHVNVVVGM
                     FLAGVSGSSTADAAAITTVFAPAMKQRGYDEHFAVCLSACSSTMGAVIPPSILMVIYG
                     ATAGVSIGALLIGGILPGLLIGLALMAQSHVFAVRHGFPREHPRFLGLRAVWQGVVAS
                     AWPMGVPLIIVVGMVGGVFTPTESAAVAAVYALFVTLVVYRSLRWRELPALLGETAVQ
                     FSQVLFCVGGAALWGWILAFYQVPDIVSEFILGFTRDWVSIMLLIVLVNVLLGTFMDA
                     IPAILIFVPIVHPLAVEVGIHPVHLGVVVVMTQAFGLLTPPLGMCAMTACAVAGVPLA
                     RIQGLLHVMMLPILLICFLCALAPWIVLWLPRLLVPNWV"
     gene            17639..18700
                     /locus_tag="VD791_04075"
     CDS             17639..18700
                     /locus_tag="VD791_04075"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXV07180.1"
                     /translation="MQPVDTLETPAVTVDLDIMEDNIRRVQRQLDKYGIGNRPHIKTH
                     KIPALGKLQMQAGAAGITCQKLGEVEVFTDAGVADDVLLTFNIVGRQKTDRLMAQMKR
                     LKRLAVVLDNEPVARGLSEAAVRHGMDVRFLVECDTGMGRNGVQTPQQALDLARAAMR
                     LPRLQFEGLMTFPLRHPSSFDFFTEALSLFRQAGIRVPVVSGGGTPAIFSVDKFPMLT
                     EHRAGTYLFNDVMTVASGTATWDNCAMRVRATVVSRPTPMRAVIDCGTKVLTSDLYTV
                     TGYGHVLEYPDAVVPHLSEEHGVIDLSRCKTGPEIGETVHVVPNHCCVVSNMVDELYG
                     VRRGQVEATWPVAARGAVR"
     gene            18713..>19712
                     /locus_tag="VD791_04080"
     CDS             18713..>19712
                     /locus_tag="VD791_04080"
                     /inference="COORDINATES: protein motif:HMM:NF005566.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HXV07181.1"
                     /translation="MPAQPRALVIGGSLGGLFAGLLLQRAGWHVEIFERVPEALAGRG
                     AGIATHPELLEILARCGARADGSVGVEVPHRIVLDASGRVIAERAMPQVFTSWGRLYA
                     LAKAQFPAAQYRTGYSLERVEQRGETVTACFADGRRAEGELLVGADGIRSTVRAQFLP
                     AVKPLYAGYVAWRGMVEEAAISAATRASLMNRFAFCLPPGEQMLGYPVAGPANSTRPG
                     ERRYNFVWYRPAAEEPDLADMLTDTTGYRHEVSIPPPAIRPDVIADMRRAAENLLAPQ
                     FAEILGLIREPFFQPIYDLESPQLAFGRVALLGDAAFVARPHVGMGVTKAAEDALGL"
BASE COUNT         2958 a         6874 c         6959 g         2921 t
ORIGIN      
        1 tcggcgcagc gcgcagctcg gcatcctggc gctgtttctc gccggaccct ggttcggcat
       61 ctggtggctg aagggcaatc tcgcttccag caccttcctc gacgtcatcg gcttgaccga
      121 tccgctggtg ctgctgcagt ccttcgccgc cgggcacaaa gtcgcagccg ccgcgctgat
      181 cggcgcagtg acgatcctgc ttgcctacgt ggtgctgggc ggccgcgcgt actgctcctg
      241 ggtgtgcccg atcaacatcg tcaccgacgc cgcgttctgg ctgcgcagcc gcttgcgcat
      301 caacgccagc tggcaaccgc gcaagagcac ccgggtatgg atcctggcgg tcgcgctggc
      361 ggtgtcggcg ctcaccgcca ccatcgcctg ggagctggtc aatcccatca ccatgctgca
      421 gcgcggcctg gtgttcggca tcggcctggc ctgggccccg gtgctggcgg tgttcctgtt
      481 cgacctcgcg gtaagccggc gcggctggtg cagcacgctc tgtccggtcg gcgcgttcta
      541 tgcgacggtc ggcaaggcgt cgctggtgcg cgtgggcgcg gtgaagcgca cggcgtgcac
      601 cgactgcggc gagtgcttcc gcgtctgccc cgagccgcac gtgatcaccc cggcgctcaa
      661 gcccaggcgg ccggacgcct cgccggtgat cgcgagcggc gactgcacca actgcgggcg
      721 ctgcatcgac gtgtgccccg acgacgtgtt ccggatgggg acgcgcttcg ccaagctgtc
      781 gcgcatcccg gccacgacgc tcgcggtcgc gctggctgcg ctgtgcgtcg tcgcgggccc
      841 ggtgcaggcg aagacgttcg tgtacgccaa ccagggcgac acgttgtcca tggacccgca
      901 catgctcaac gagtcgctgc tgctcaattt caccggcaac gtgtacgaat cgctggtcgg
      961 gcgcggccga aagctcgagt tggaacccga gctggccacc gactggaagc gcacctcggc
     1021 cacggtgtgg cgcttcaact tgcgcaaggg cgtgaagttc cacgacggca ccccgttcac
     1081 cgcggacgac gtgatcttct cctggcagcg ggcacgcggc gagggctcgg acatgaagac
     1141 ctacgtcggc cagatcgccg agatccgcaa gatcgatgcg cacgcgatcg atatcgtcac
     1201 cagcgagccg ttcccgatcc tgcccgacgt gctgacgctg tggcgcatca tgtcgaagag
     1261 ctggtgcgag aggaacaacg cggtgcgccc ggtggacgtg cgcagaggca cggagaacca
     1321 cgccagcaat cacgccaacg gcaccggacc gttcatgctc cagtcgcgcc agcccggcgt
     1381 gcgcacggtg ctggtggcga accccggctg gtggggcaag cccgagcaca acgtcaccga
     1441 ggcgcggttc ctgccgatcg ccaacgaggc cacccgcgtg gcggcgctgc tgtcgggcga
     1501 gatcgacatg atggagccgg tgcctctgca ggacgtgcag cgcctgcgca acgacgccag
     1561 gttgaaggtg atgcagagcc cggagctgcg caccatcttc ctcggcatgg accagaagcg
     1621 cgatgcgctg atctattcga gcgtgaaggg ccgcaacccg ttcaaggacc ggcgcgtgcg
     1681 gcaggcgttc taccaggcga tcgacatcca ggcgatccgc tcgcgcatca tgcgcggcgc
     1741 cgccacgccg accgcattga tgatcgcgcc cgggatcaac ggcttcgacc ccgctctcaa
     1801 tcagcgcctg ccctacagcc ccgaggcggc caagaagctg ctggccgagg ccggctaccc
     1861 gaacggcttc gaggtcggca tgaactgccc caacgaccgc tacgtcaacg acgccgagat
     1921 ctgccaggcg gtggcggtga tgctggcgcg catcggcgtg aaagtgaacc tgatggcaga
     1981 gaccaaggcg acgttcttcc ccaaggtgct gtcgcgcgaa acctcgttct acctgctggg
     2041 ctggacgcct ggcacctacg acagccacaa cccgctgttc tcggtgctgt ccacgcccgg
     2101 cgagggcggc caggggcagt tcaacctggg cgcctactcg aatgcgcgcg tggacgagct
     2161 gacgcgcgcg atcggctccg agaccgatcc ggtgaagcgc caggcgatga tcggcgaagc
     2221 gttcaagctg cacgccgagg acatcggcca cctgccgctg caccagcaga tgctcacctg
     2281 gggcatgaag aaaaacatcg agctgacgca gcgcgcggac aacttcaact acctcaagtg
     2341 ggtggtgatc cgctgaatgc gtgaagcgtg gctccgcgcc tgggactcgg acgtcgccta
     2401 cagcttccgg cactcgccgg tgacgatggc ggcggcgctg gccacgctgc tgtgcgtggg
     2461 cgcggccctg ttcgcgccct gggtggcgcc gcacgatccc ttcgatctcg cctcgctgga
     2521 cctgctgaac gccttcaccc cgcccgcctg ggacgcgcgc ggccaggcga gctacccgct
     2581 gggcaccgac gatcaggggc gcgacgtcct gtccaccatc ctgtacggcg cgcgcatctc
     2641 cctcgcggtg ggcgtggcct cggtcgtttt cgcgctggtg ctgggcgtcg ggctcgggct
     2701 tgcggccggc tacgcgggcg gcaggatcga cgccctgatc atgcggctgg cggatgtgca
     2761 actgtcgttt ccggcgatcc tgatcgcgct gctcatcgac ggcctggtgc gcgtggcgct
     2821 gcccgccgag cgtcacgccg acatcgcggt gtacgtgctc gtgctcgcga tcggcgtgtc
     2881 cggctgggtg cagtacgcgc gcacggtgcg cgggtccacg ctggtggaga agagcaagga
     2941 gtacgtgcag gcggcccgcg tcatcggccg gcggccggcg gcgatcatgc tgcagcacgt
     3001 gctgcccaac gtcctcggcc cggtgctggt gatcgccacc atccacctgg ccaccgcgat
     3061 catcaccgag gccacgctgt cgttcctggg cgtgggtgtg ccgcccacca cgccgtcgct
     3121 gggaacgctg atccgggtcg gcaacgacta cctgttctcc ggcgaatggt ggatcgcggt
     3181 gttccccggc atcgcgctgg tcgcgctggt gctgtcggtg aacctgctcg gcgactggct
     3241 gcgcgatgcg ctcaatccca ggctgcggtg agtccggcgt cgcctctgct cgaagtgaag
     3301 cgcctgcgcg tggagttcca gacgcggcgc ggggcgctgc tcgcggtgga cgacgtgtcc
     3361 ttcgcgatcg gccgcggcga agtgctgggc gtggtgggcg agtcgggcgc gggcaaatcg
     3421 ctcaccggca tggcgatcat cggcctgctg gagccgcccg gccggatcgc gggcggtgag
     3481 gtgcgcctcg acggacagcg catcgacgac ctgccgcagg ccgagctgcg gcgcatccgc
     3541 gggcggcgca tcggcgcgat cttccaggat ccgctcacct cgctcaaccc gctgtacaca
     3601 gtggggcagc agctcgtcga gacgatccgc acgcatctgc ccgtctcgca gcgcgaagcg
     3661 cgggcgcgcg cgatcgccct gttgcgcgag accggcatcc cggcgccgga gcagcgcatc
     3721 gaccagtacc cgcatcagtt ttccggcggc atgcggcagc gggcggtcat cgcgctggcg
     3781 ctgtgcgccg ggcccgagct gatcgtcgcc gacgagccga ccaccgcgct ggacgtctcg
     3841 atccaggcgc agatcatcgg cctgctcaag cgcatgtgcc gcgagcacgg cgcggcgatc
     3901 atgctgatca cgcacgacat gggcgtgatc gccgagacgg ccgaccgcgt agccgtgatg
     3961 tacgccggcc gcatcgccga gatcgggccg gtgcgggagg tgatccacgc gccgcgccat
     4021 ccctacagcg cgggcctgat gggctcgatc cccaagcccg ggcaggcgcg cgcgacgctg
     4081 gtgcagatcg acggcgcgat gccgcgcctc aacgccatac cctccggctg cgccttccac
     4141 ccgcgctgcc cgcggcagtt cgcccgctgc acgcaacagc gcccggagct gatcccggcg
     4201 ggaagcagcc gcgccgcctg ctggctgcac gccgatgagc gctgaggcgg cggcgctgct
     4261 ggtgctgcgc gatgtcggcc gcgacttcga tgtgtcgcga ccgtggctcg agcgagcgct
     4321 ggagcgtacc ccgcgtcggg tgctgaaagc ggtggacggc ctgtcgctcg acatccgccg
     4381 cggcgagacg ctggcgctgg tgggcgaatc cggctgcggc aagtccacgg tggcgcgcat
     4441 gatcgtcggc ctgcacgccc ccagccgggg gcgcatcgag ttcgagggcg tcgagctcac
     4501 cgccgcgcgg cccgcgccgg aagtcgcccg cgcgcgccgg cgaatgcaga tgatcttcca
     4561 ggatccctac gctagcctga acccgcgctg gcgcgtcgag cgcatcgtgg ccgagccgct
     4621 gcgcgcgcag tcacacgccg ccggccgcga cgcgatccgc gcgcgcgtgg cggagctgct
     4681 gctgcaggtg ggccttacgc ccgaggacgg cggcaagtac ccgcacgagt tctccggcgg
     4741 acagcggcag cgcatctgca tcgcccgcgc actgtccgcg cacccggagt tcctggtgtg
     4801 cgacgagccg acctccgccc tggatgtgtc ggttcaggcg cagatcctga acctgatgaa
     4861 ggcgctgcag cagcgcctgg gactgaccta tctgttcatc tcgcacaacc tggcggtggt
     4921 ggcctacatc gccgggcgca tcggcgtgat gtacctggga cggctggtgg agctggccga
     4981 cgccgcgacg ctgttcgcgc agccccagca tccctacacg cgcatgctgc tggacgcggt
     5041 gccggacctg cagatgagcg gacgcccgcg cactccggtc gccggagaag tgcccaatcc
     5101 gctcgacccg ccctccggtt gcgcgttcca cccgcgctgc ccgcacgccg acgcgcgctg
     5161 ccgtgccgag cggccggcgc tacggcgcgc ggcggcaggc tcgatggtgg cgtgtcacgc
     5221 ggtggaggaa cagcgcctgc cggcctggac cgggcggttc tcgtcgaccc cgcagcgcgc
     5281 gggcgtgcaa tgactcaccc cccggccgga gcaagccgca gcacgcgtcc gtccgagctg
     5341 tcggtcagca gatagacgct gccgtcggcg ccctgcgcga cgtcgcgaat gcgcgcgctc
     5401 agttcgcggc cgcgatagcg ctcttcggcg gccacgcgcc cgtcgcggag caccaggcgg
     5461 accagtccgc cggggttgag cgaaccgacc agcagattgc cgtgccaggc ggggaaggcg
     5521 gtgccggtgt agaacaccgc ccccgaaggg gcgatcaccg gatcccagta atacaccggc
     5581 tgttccatgc cggctttggc cgtgccttcg ccgatcttcg cgccgctgta gtcgacgccg
     5641 taggtgatca ccggccagcc gtagttgctg ccccgctgcg gctggttgag ctcgtcgccg
     5701 ccgcgcgcgc cgtgctcgac ggtccacagt tgcccggtct cgggatgcag cgcggcggcc
     5761 tggatgttgc ggtggccata cgaccagatg tcgggccgcc cgccgccctg cacaaacggg
     5821 ttgtcgccgg gcgccgagcc gtcggggttg aggcgcacca ccttgcccag cccggcctcc
     5881 aggttctgcg cctgctgccg gtatccggcg cgatcgccga gcgtcacgaa cagcgtgccg
     5941 tcgggcgcga acaccagccg cgagccccag tggttcgcgc cggcgacttt cggctgctgg
     6001 cgccacacga cctgggtgtc ctccagcccg cgctcgccca ggcggccacg cgcgaccgcg
     6061 gtgcctgcac cgccctcgcc gggttcggag aacgacaggt aaaccagccg gtcgcgcgca
     6121 aagtccggac tgagcgcgac gtccagaagg ccgccttgtc cgcgcgcgta cacctcgggc
     6181 acgccggcga gcggcgcgct cactttgccg tcggcggcta tgcgccgcag ctgccctgac
     6241 cgttcggtga ccagcatgcc gccctcgggc agcagcgcca gcccccaggg gtgctccagg
     6301 ccgcgcgcga tgacggtgac gctgcccggg aaggacttgg cgtcgggggt gggcgagcgc
     6361 ggcgcctggc cggacgcggg cagcgcatgg gtcgacagca gcgcggcaag cgcgatcgct
     6421 cgcgtgcacg gtggcgcgcg gcggatgagg gaagaaaggc gaatcatgtc agcctcctct
     6481 cgtcgacgcc ccgctgatgc ggccggcgcc aggcgcgcaa tccgggcgac gcgggaattc
     6541 gcggcgccgt ctacgcgggg gttcgcggcg cgccgcgcag cagcgtgccc aggtgtccga
     6601 cgtcctcgct cagctcaagg cgatctatga agccttcgat ctgtcccgcg cgcgcggccc
     6661 cgagcacgcg cgccgcggca ccccgtaagc gcgtgcgcac ctctgcggcg gtggcgttca
     6721 ccacgtcgtc caggcggacg cgatgcgtgg cccccgacgc ctcccgcact tcgacctcgg
     6781 cgccctgctt gcccgggtag gcgcgggtca tgtcgtcgtc cacctgcagc gtggtgaggc
     6841 cgatcagccg gcgcacctgc gcgtcgtcga gcgcggcaaa gctgtcctcc gacagctcgc
     6901 cccggatcag cgccgccgcg acgttgtact ggatgctcat cttggcctgc agtacgtgcg
     6961 cgaacgggcc gcggtaatcg cagcccggat aggccgcacc ggcgcgcgag acgcgcacca
     7021 ccacggccgc gatccgctcg ggacggcagc cgctgtcgcg cgcgatgcgc agcgccgcct
     7081 gggagggcgt ctgcgcgaaa ttgcaggcgg gcaacggctt gtgatacacg gagagcagct
     7141 ccggtggccc ggcgaagagc cgcacgcgcg ccgcggcctc acgcttgccg agcgccgcga
     7201 acagaccggc ctcgccgtcc agcccggtgg gcgaggcgaa cgcgccgagc ccggccagcc
     7261 gcacggccgt gagcgcgttg cgcgcggcga acccggcgtg gaagaacatc tcgctgccgc
     7321 cggtgtgcgc ccattgattg aagccggccg tggtgttagc ggcgagcacc agcgcgctgg
     7381 cggtctgccc ggcgtcgaag ccgagcaggc gcgcccccgc cgccgcggcc gcgagcggac
     7441 cgctgatgcc ggtgggccgg aagatgcgcg ctaccgcagc gtccatcagc acgcggccga
     7501 tctggcagcc gacttcgtag ccggccaccg tggcggcgat gaagtcgcgg ccggccaccg
     7561 ggcgcgactg cgccagcgcg agcagcgccg gcagcaccac gatgcccaga tggctgatgc
     7621 tgccggcatg catgtcctcg cgcaccagcc catgccccat cacggcgttg gcgaacgccg
     7681 cgtcgccggc cgccgccggg cgcggcgagc cgatcacggt ggccgcgccc gcgccttccg
     7741 ctaccggcgc ggcgagctgc acggcctggc ggctccacgg cagatcgcgt gcctcgaagg
     7801 cgcaaccgat gaggtccatc aggcacacct tggtcttctc gatgactgcc tccgggatct
     7861 tgtcagcgac ctcccccgac agctgcgccg tcgacaactg cgccacggtg cgggcgatgc
     7921 gcgtggcaag cgcctcttcg gcgccgctgg tggcgcgcgt cccggtcgtt tgcactgcgt
     7981 tctgcatggc atcgcctcct ttcagccgcg gacttgcacg ccggcccagc tctccaccac
     8041 ccggcagatc gaggtgaaat ccgaatcgcc gccgtacagc gcgttggtca ccgctagcat
     8101 ctcgcgcacc gcggcgccgg ccaccatcgg cacgcccatc gcttcggcct cctcgatgca
     8161 cagccgcacg tccttgtagg acagcccggt ggcgaagccg aagtcgaacg ttccgggcag
     8221 cacggcgcgc gggaacttgt cctggctggc gctgttgcgg cccgagccgg cgttgatgac
     8281 gtcgagcatg gtcttggggt cgaggccggc cttcacgccc atcaccatcg cctcggaggt
     8341 gaccgccagg gccgaggccg ccagcaggtt gttggccagt ttcatggtct gcgcctgacc
     8401 ggctttctcg cccaggtgga acggcttgcc gaaggtcttg agcagcggtt ccagctccgc
     8461 gaacagcgcc ttggggcagg acaccatgac cgccagcgtg ccctgggccg ctccggcgat
     8521 gccgccgctc accggcgcat cgacgtaggc gatgccgcgc tcgccgagcg cggccgccac
     8581 cgcctccgcg gtgcgcgggc cgatggtgga gaagtcgacg acgcgcttca cccgcttgcc
     8641 gtcgatgatc cccgccttgc ccagcgcgac gctgtgcacg atctcggggt tgggcaggct
     8701 gaggagcacc gttgccgcga gcgaggcggc ctcttcggcc gaggcggccg cgcgtgcgcc
     8761 gtctttggcg agcgcgtcca tcgccgcctg gctacggtcg aacacgcata cccgatggcc
     8821 ttgcgcgagc aggcgcctgg tcatgggcgc ccccatgcgc cccactccga cgaagccgaa
     8881 cgtctggctt tccatgcaac ctcctggtgt ttcccgtctt acccctcacc ccaaccctct
     8941 cccgaccctc accctaccct ctcccgcgag cgggagaggg acctgtgggt ctatgggaga
     9001 gggagcaagt gtgctccacg ctgcgttggt gaagtccact actgacggta gcgcttgatc
     9061 acttcgggat ccggatccag gcccaacccg ggacccagag gcaccgcgat cgacccctcg
     9121 cgcggcacgg ccgcatcgcc gtacaggcgc gcctcgagat cgaaatagcg ccactccacc
     9181 agcgaccctt gccccagcgc ggcgttcacg tgcagcgccg cgagcaggcc gggaccgtcg
     9241 tagaagctgt gcgggaccac cgtcacattg tgcgcggcgg cgagcgcgaa caccttgcac
     9301 agctccgaga tgccgcccat cttgatcggg ctcggctgcg cgaagtccac cgcgccggct
     9361 tcgaacatgt gccggtactg ctgcaaggtc gcggcgtttt cgcctgcggc gatcgggatt
     9421 ccgccgcgcg cgcgcacctg cgccagcgcc gcgtagtcct ccggcggcca gaccggttcc
     9481 tccagccagc gcaggtgcaa gggccgcagc ttctcggcca tgtccagcgc ttcgcgcagc
     9541 gaccacgggc agttcacatc gagcatgatg tcgacgtcct gcccgcccgc gtcgcgcgcc
     9601 gcgcgggtgg gcgcgaccgc gatctcgtgc agcttgacgt ggcggaaccc ctggctgagc
     9661 gcgcgcgcga cgttgtccgc caccaacttg ggatcgctgt agcggatcag gctggcatag
     9721 cagggcaggc gcggcagccg gcagccgccg agcagccggt gcaccggctg tcccgcggcc
     9781 ttcccgacga tgtcccagag cgcgatgtcg atccccgaaa ggccgaagat caccggcccg
     9841 ccgcgcccga agatgtgcag cttctgctgc agcgccagcg tcaggctctc gatctgcagc
     9901 gggtcgcggc ccacgcacat cggcgccagc atgtgctcga tggcggcccg ggtggccggg
     9961 atggcgttgt acccgaaggc ctcgccccag ccggtcaggc cggcgtcggt gctgatcttc
    10021 accagcagcg catccgcggt ctcccacggc ttgccgcccc aggcctcggt gtcggtgctg
    10081 ccgccgcagg tgtaggggat gcgcaggacg atggtttcga cggcttcgat cttcatgcgg
    10141 tggggcgggg ctcggtccag acgtggcgca atgataggcg agggcgcgac ggatcgctcg
    10201 tggcggtcag gcgagcaccc cgaagaacag ccagcacacc agggcgccca ccacgctcat
    10261 cgacaagccc caggcgagca tgcggttgaa cagcgccttg cggtcttcct cgggcgccgc
    10321 ggcggcgatg cacagggcgc ccagggtgga cagcggggag acgtccacca ggtgggcacc
    10381 tacgttgatc gaggcggcga tgccgagcgg atcgccgccg ccgagcttgt cgaccaaccc
    10441 cgggatggtg ggcaggaagg cggggagcac cacgcccgag gagctgctgt acaccgagat
    10501 cacgccggtg acgaaagcga tgacgccggt gatcgtctcg cgcgtcgaga actgcgcgag
    10561 cagcgtggtg aacagatcca tgccaccggt cttctccacc agggcgatca gcacggtgac
    10621 gccgcacacc atcaggatgg tggcccaggg catggccgcc accgcggcgc gttcctgggc
    10681 ggccccggcg agcgacagca ccaccgccgc gacgaacgcg ccgaaggtca cgtccacctt
    10741 gaacaccgcc acgctcacga tcaacgccgc gatcacggcc agcgtgagct tctgccgcca
    10801 gtcgaacggg gtgctggccg gctgtaccgc gccgcccgtt gtatccacca ccacgccggg
    10861 tgcgtccgcc cggggttggc gcatcagccg ccagccgccg agcgcgagat agcccgccag
    10921 cgcgacgacc gtctgcggca gcagagcgtt gaagtagttg cgccactcgt ggccctccag
    10981 accgatgcgc gccatcagcc cgttggcgat gatgccggtg ggcgcgaacg gcgacagcgc
    11041 cccggcattg gcgccgttac acaccatcag cgccatcagg aaggcgggaa tgccggcgcg
    11101 gccggcggcg gccatcgcca ccggcgctag cagcgcggtg gtcgcgatgt tgcccggccc
    11161 gagcgtggca aacgcgagcg ccaggccgaa aaaggcgaaa gggatatagc ccggatgccc
    11221 ggccgccacg cgcaccgcga cgctcgacac gcgttccagc gtgccgttgg cccgcgcttg
    11281 cgcgaacagg aaggtgatcg caaccagcgt caggaacagg ttcaccggaa agccggcggc
    11341 cacgtccttg agcggcaggc cgccgatcag cacgccgatc gcgaacgcgc cggcgatgga
    11401 caggatgccg acgttggcct gggtgaacgt gcccagcacg atcgccagga acaaggcgat
    11461 cagcgacaac accgccagat tcacgctcgg ctcctcgtca gccaacctcc ggaatgggtg
    11521 gcggcgccag cataccgcca agcgcgctcg gggacatgcg ggcggcgggg agtgggtccc
    11581 gaaaggggcg gaccgcggcg cgtattctca agcgccgtcg gttccaatac catggcggcc
    11641 aggagcgaaa aagggaggag agatgccccg cgtgccggtt gtcgtgctag ccctgctcgg
    11701 gacgctgtcg ctttgcgcgc ccgcagcgcg cgcgcaggaa gtgacgctgc gcgcgttgac
    11761 gtccttctcc gagggcacgt tcttctcgcg caacttcgag cgcctgatcg agaagatcaa
    11821 ccgcgaaggc aggggcgtgc tgcagatccg ctacgtgggc ggaccgaaag cgattcccac
    11881 gatggaggcg ggcaacgcgc tgcgctcggg cgtggtggac atcgccaaca tcgccagcgc
    11941 cttctacgtg aacctggtgc ccgaggtcga tgcccagaag ctggcgcgcg tccccaccgc
    12001 cgagcagcgc cgcaacggcg cgttcgcgta cttcaaccgg ctgcacaacg aaaagatgaa
    12061 cgccgtgtat ctggccaggc agcacggcaa catccagttc cacctgtacc tgaacaagcc
    12121 gatcgagcgc tccgatctga gcggcctgaa aatccgggtg acgccggtgt accgcgactt
    12181 cttcggcgcg ctgggcgcga ccaccgtgac cacggcgccc ggcgaggtgt acatggcgct
    12241 ggagcgcggc gtggtggacg gctacggctg gccgtcggtc ggcatcttcg acctggcctg
    12301 gcacgagcgc accaagtacc gggtcgagcc ggcgttctac gtggtcgacg tggcggtgct
    12361 ggtcaacctg gacaagtgga acggcctcac cgcgcagcaa cagaagctgt tgagcgacgc
    12421 ggcactgtgg ctcgaggggc tcgaccgcga gaacgacgcg ctggtcgcga aggagctcga
    12481 gcgccaggcg caggccggca tccggccgat ccggctggaa ggcgcggagg cgaagcgcta
    12541 tctggagcgc gcccaccagg tggggtggga cagcatcgcc aggatcagcc ccgcgcacgc
    12601 ccccaagttg cgcgcgctgc tggacaagca gtgagcgaaa ccacgacggc gatgcgactg
    12661 gaaggcaaga tcgcgatcat cgtcggcgcc gggcagagcc ccggcgaagg catcggcaac
    12721 gggcgcgcca ccgcgctgcg gttcgcgcag gagggcgcca gaatcctggc ggtggaccgt
    12781 gaccttgcct cggcccggga aacggccgcg atggtgacgg cggagggcgg cgagtgcctg
    12841 gccttcgagg ccgacgtcac caaagagcag acgctcgccg ccgcggtgtc cttcgcacag
    12901 gcgcgctggg gccgcatcga cgtcctgcac aacaacgtcg gcgtgagcgt gggcggcggc
    12961 gacgcgccgc tcgccgagct caccgaggaa actttcgacc gcatcagcgc gatcaacctg
    13021 cgcggcacca tcatggcgtg caagcacgtc ctgccggtca tgcgccagca gcgcgacggc
    13081 gcgatcgtga acatctcctc ggtcgccgcg tggaccgagt atccgttggt ggtgtacaag
    13141 gcgaccaagg cggcgatgat cgcgttcacc cagcagctcg cgatccagaa cgcggcctac
    13201 ggcatccgcg ccaacgtcat cctgcccggt ctgatggaca cgccgatggc ggtggacacg
    13261 cgcgcgcggg cgagccgccg cagtcgcgcc gaggtggcgg ccgagcgcga cgccaaggtg
    13321 ccgctgcgcc agcgcatggg cagcgcctgg gacgtggcca acgcggcgct gtttctcgcc
    13381 tcggacgagg cgcgcttcat caccggcgtg gcgctgccgg tggacggcgg cgcgctggtg
    13441 cgcatcggct gacactcacg cgccgctgtc acgttgcggc aacgagccgc tgccgcccct
    13501 ggcgcagcag ccaggcggcg atcgcgacgt tgagcaggtt ccacagcagg ccgttgacga
    13561 aggcggcgtc gtaggagccg gtgaggtcga agatggcgcc ggacatccat ccgccgagcg
    13621 ccatgccgaa cagcgtggcc atgaacaccg cacccacccg cgcgccggcc tcctgcgccg
    13681 gaaagtactc gcgcacgatg atggcatagc tgggcacgat gccgccctgg aacagcccga
    13741 acagggcgga gatcacgtac agcgacacca tcccgtcgaa gcccaggaac agcgccagcg
    13801 ccagcatctg caggctggaa ccgaggagga gcgtgcgcac gccgccgatg tgatcgacga
    13861 tccagcccga ggcgaggcgg ctgacgatcc cgaacccgag catcagggaa agcatctgcg
    13921 caccgcgcgc cgcgccgtaa ccgagatctc cgcaataggc gacgatgtgc acctgcggca
    13981 tcgacatggc gatgcagcag ctcagccccg cgaggatcag caacgcctgc aacgcacccg
    14041 gcgacagccc gaccgacagg ggcgaggcag gcgctgcgcc caagctggag ggccgcgctt
    14101 tggagacaac cggcgcgtcg cgcggcgagc gcctgcgcag caccgccgcc gcaagcggca
    14161 gcaccgtggc cacgcagaac aacgcgatcc cgaagtaggt ctcgcgccaa ccggcgctct
    14221 cgatgtaatg ctgcaccacc ggcggccaga cggtcccggc gagatagttg ccgctggcga
    14281 agatcgccac cgccatgccg cggcagcgcg cgaaccacat ggacgtgtcc gccaacagcg
    14341 gcgcgaacgt cgcggaggag cccacgccga tcagcaagcc ctgagcgagc gtgaactgcc
    14401 acagatgcgc ggacagtccg gcgcaggcgt agccgatggc gagcgcgatg gtgccggcga
    14461 tcaccggcag caggatgccg aagcgatcgg acaggcggcc catcaatacc ccgcccaggc
    14521 cgtagccgat catggtcagc gtgtacggca gcgacgcgtc ggagcgcgcc gcggcgaact
    14581 cggcttgcac cggcggcagc acgaccacca ccgaccacat gccgacaccg ccgatggcgg
    14641 agagcagcag cgcggccgtg agccgcgccc aggcgtaggc ggagtccacc ccgagtttgg
    14701 cgtccggcgc gtgcccgggg ccgattacgc ccgtcatttg tgcaagcagg ctcctttgta
    14761 cgatgatact aacatccgat gaatcgggct ccgggaggag gcgttcgatc atgcacaacc
    14821 tgcctgcggt cctcgcgctg ctgatggcag ccggcctggc tgcgattccc gaccccgccg
    14881 gcgcgcagca gatccgcatg cgcttcggtc acgaccagcc cgtgggcagc atgtacgacg
    14941 aaggtcacac catgctgcgc aagctgctcc aggagcgcgc cggcgagcgc gtgcgcatgg
    15001 acatctttcc cgcggcgcaa ctcggctcgg aagtggcgat gatcgaaggc gtgcgcctgg
    15061 gatcgctcga cggcagcgtc gcgcacgtgg cgaacgccgc caccgtggtg ccggagctgg
    15121 cgctgttcag cgtctcctac ctgttcaagg atcgcgagca ttacgagcgc gtgctgaacg
    15181 acccgcgctt ccgggagcgc atggatcgcc tggtggcgag caagaacctg gggctgcggc
    15241 tgatcggctt ctactccgcc ggggtgcgta tgctctacac ccggcgcggc ccggcgtaca
    15301 ctccggagga gctgaaaggc accaagatcc gcgtcatgaa caacccggtc gaggcgcgca
    15361 tctggcaggc gctgggcacc atccccacgc cgatgaactt cggcgaggtg taccaggcgc
    15421 tgcagtcggg cgtgctggac gccgccgaga acgcgccgtc ggtgatcgag tcgaaccggc
    15481 actacgaagc ggcgaagacg ctgatcctca ccgagcacca gcgctccatt tgcctgctgc
    15541 tgatcaacga gaagaagttc tccggacttc cccccgatct gcagaagaca ctgctggagg
    15601 cggccgcgca ggccgcggcc tacgaacgca agcgcgacct cgagttgaac agcgaggcga
    15661 tcgcgcgcat gcaggcgcgc ggcgcgcggg tggtgacgcc ggagcgggcg aagttcatcg
    15721 cgcgcatcgt gccgatccag gaccaggtcg cacgcgagct gaagatgacc gatgtcctgg
    15781 agctgatccg cggccatgcg cgctgagcgg gtgttcgagc ggctcgagcg ctggctgctg
    15841 ccgctgctcg cgagcctgct cgcgctgatc accgccggcg tgttcgtcca ggtggtgctg
    15901 cgctacggct tcgccaagtc tttcctgtgg ggcgaggagc tggcgctgtt cgccttcatc
    15961 tggtgcgtgt atctgggtgc ggcgatctgc acctggcgcc gcagccactt cgcgttcgac
    16021 ctgttcgccg gccggttgtc gggccgcgcc gccggagcgc agcagctggc cgtggacctg
    16081 tgcgtgctcc tggtcgccgc ggtgatgctg gtccagggcg cggccttcgc cgagctaagc
    16141 gtgcagcgcc tgtcgcccgc gctcggcatc accctgctgg tgcccacgct cgccatcccg
    16201 ctgagcggcg gcctgatggt gctcgccgcg ctggcggcgc tgcaccgcga cctgcgtctg
    16261 atcctgaccg ggcgcgccgg ttgatgcatc cgctgctgct gctggtggga tcgctcactc
    16321 tgttgctgat cgccggggtg ccgatcggct tcgcgctgct gctgtcctcg ctgctggtga
    16381 tctgggtgga ggagctgccc acggcggtga tggtgcagca gatgttcaac ggcatcaacg
    16441 ccttcaccct gctggcgcta ccgttcttct tcctcgccgg caacgtcatg accggcggcg
    16501 ggatctcgga gcggctggtg aagctggcga tggcgctggt gggccatcgg cgcggcggtc
    16561 tggcgcacgt gaacgtggtg gtcggcatgt tcctcgccgg cgtctcgggc agcagcacgg
    16621 cggacgccgc cgcgatcacc accgtgttcg ccccggccat gaagcagcgc ggctacgacg
    16681 agcacttcgc ggtgtgcctg tcggcctgca gctcgaccat gggcgcggtg atcccgcctt
    16741 cgatcctgat ggtgatctac ggcgcgaccg caggggtgtc gatcggcgcg ctgctgatcg
    16801 gcggcatcct cccggggttg ctgatcgggc tggcgctgat ggcgcagtcg cacgtgttcg
    16861 cggtccggca cggcttcccg cgcgagcatc cgcgcttcct ggggctgcgc gcggtgtggc
    16921 agggcgtggt ggcctcggcg tggccgatgg gcgtgcccct gatcatcgtc gtgggcatgg
    16981 tcgggggcgt gttcacgccc accgagtccg ccgcagtggc ggccgtgtac gcgctgttcg
    17041 tgaccctggt ggtataccgg agcctgcgct ggcgcgagct gcccgcgctg ctgggggaga
    17101 cggcggtgca gttttcgcag gtgctgttct gcgtcggcgg cgccgcgctg tggggctgga
    17161 tcctggcgtt ctaccaagtg cccgacatcg tgagcgagtt catcctcggg tttacccgcg
    17221 actgggtgtc gatcatgctg ctgatcgtgc tggtgaacgt gctgctaggc acgttcatgg
    17281 acgcgattcc cgcgatcctg atcttcgtgc cgatcgtgca tccgctcgcg gtggaggtcg
    17341 gcatccaccc ggtgcatctg ggcgtggtgg tggtgatgac ccaggccttc ggcctgctca
    17401 ccccgcccct gggcatgtgc gcgatgaccg cgtgcgccgt ggccggcgtg ccgctcgcgc
    17461 gcatccaggg gttgctgcac gtgatgatgc ttcccatact gctgatctgc tttctgtgcg
    17521 cgctggcgcc ctggatcgtg ctgtggctgc cgcggctgct cgtgcccaac tgggtctgaa
    17581 gcccgccaac cgggtctgaa aaaccgccaa ctgggtctga aaaccgaagg agaacgtgat
    17641 gcaaccggtc gacaccctcg aaacccccgc tgtgacggtc gatctcgaca tcatggagga
    17701 caacatccgg cgcgtgcagc ggcagctgga caagtacggc atcggcaacc gcccgcacat
    17761 caagacgcac aagatcccgg cgctgggcaa gctgcagatg caggccggcg ccgcgggcat
    17821 cacctgccag aagctgggcg aggtggaggt gttcaccgat gcgggcgtgg ccgacgacgt
    17881 gctgctcacc ttcaacatcg tcggccggca gaagaccgac cggctgatgg cgcagatgaa
    17941 gcggttgaag cggctggcgg tggtgctgga caacgagccg gtggcgcggg gcctttccga
    18001 ggcggcagtg cggcacggca tggacgtgcg cttcctggtc gagtgcgaca ccggcatggg
    18061 ccgcaacggc gtacagacgc cgcagcaggc gctggacctg gcgcgcgcgg cgatgcggct
    18121 gccgcgcctg cagttcgagg gcctcatgac ctttccgctg cgtcatccga gctcgttcga
    18181 cttcttcacc gaggcgctgt cgctgttccg gcaggccggc atccgcgtgc cggtagtctc
    18241 tggcggcggc acgccggcga tcttcagcgt cgacaagttc ccgatgctca ccgagcatcg
    18301 ggccggcacc tacctgttca acgacgtgat gacggtggcc tcgggcaccg ccacctggga
    18361 caactgcgcc atgcgcgtgc gcgccaccgt ggtaagccgg cccacgccca tgcgcgcggt
    18421 gatcgactgc gggaccaagg tgctgacctc cgatctgtac acggtgaccg gctacggtca
    18481 cgtcctcgag tatccggacg cggtcgtgcc gcatctgtcc gaggagcacg gtgtgatcga
    18541 cctgtcgcgc tgcaagaccg gcccggagat cggcgagacg gtacacgtgg tgcccaatca
    18601 ctgctgcgtg gtcagcaaca tggtggacga gctgtacggc gtgcgccgcg gccaggtgga
    18661 ggcgacctgg ccggtggcgg cgcgcggcgc ggtgcgttga gagggcggat cgatgcccgc
    18721 gcagccgcgc gcgctggtga tcggcgggtc gctgggcggg ctgttcgccg gcctgctgct
    18781 gcagcgggcc ggctggcacg tggagatctt cgagcgcgtt cccgaagcgc tcgccggccg
    18841 cggagcgggc atcgccaccc acccggagct gctggagatc ctggcgcgct gcggcgcgcg
    18901 ggcagacggc agcgtgggcg tggaagtgcc gcaccggatc gtgctggacg cgtcggggcg
    18961 cgtgatcgcc gagcgggcga tgccgcaggt gttcacctcc tggggccgcc tgtacgcgct
    19021 cgccaaggcg cagttccccg cggcgcagta tcgcaccggg tactcgctgg agcgcgtcga
    19081 gcagcgcggc gagacggtga cggcctgctt cgccgacggg cggcgcgcgg aaggcgagct
    19141 gctggtgggc gccgacggca tccgctcgac cgtgcgcgcg cagttcctgc ccgcggtgaa
    19201 gccgctctac gcgggttacg tagcgtggcg cggaatggtc gaggaggccg cgatctcggc
    19261 cgccacacgg gcatctctga tgaaccgctt cgcgttctgc ttgccgccgg gggagcagat
    19321 gctcggctat ccggtggccg ggcccgcgaa cagcacgcgg cccggggagc ggcgctacaa
    19381 cttcgtctgg tatcgaccgg cggccgagga gcccgacctg gcggacatgc tcacggatac
    19441 gacgggctac cggcacgagg tctcgattcc gccgccggcg atccggccgg acgtgatcgc
    19501 ggacatgcgc cgggccgcgg agaacctgct cgcgccccag ttcgccgaga tcctgggcct
    19561 gatccgggag ccctttttcc agccgatcta cgacctcgag tcgccgcagc tcgcgttcgg
    19621 gcgcgtggcg ctgctgggcg atgccgcttt cgtcgcgcgc ccgcacgtcg gcatgggcgt
    19681 caccaaggcc gccgaggatg cgctcgggct gg
//
LOCUS       DASPGE010000129         2519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_506641, whole genome shotgun sequence.
ACCESSION   DASPGE010000129 DASPGE010000000
VERSION     DASPGE010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506641"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VD791_04085"
     CDS             <1..621
                     /locus_tag="VD791_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07182.1"
                     /translation="APDAARATRQPARHMVISSRSLARRLAAITLAAGICAGCPGFAA
                     QPAGRGAGDLACGPVFDSKGAVVLGHGTGDSVKSASKRKCPGVSTPRLAPPKARTPVI
                     MVGAQEQAESEDELAVLDTDVGRVSAAVVTGGALFWLLQSSIWTSLLILGIPLWRHVD
                     LLPIVDRASDPAPASVPPKRDASEEQAVAVMLDAQRRRRRGRRTTP"
     gene            618..2105
                     /locus_tag="VD791_04090"
     CDS             618..2105
                     /locus_tag="VD791_04090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXV07183.1"
                     /translation="MKRIRPSAQITLGLVLLTTAVLLLVDLLFNVFPDPDAQLMRVRG
                     AHAESLATQVSVLLQRGDEHSLSLALARVRARDSSIRSLAVRRVGHGLLARSGDHERA
                     WAEPRHARSALTHLLVPLTQSGTRWGSFEMTFFPDDSGPLQRVFAHPLWVTLLSTALI
                     GALVYWVYIRRALMHLDPTAVIPERVRLAFDIMTEGVAVLDNRGRLLLANSAFRALHG
                     KDGPDLVGARLSDLPWLAASLPPNAADHPWSRAMHQATPVRDVAIEVAPAADVRRKLA
                     VNCAPISDARGLVRGCIATFADLTALHFANERLTETLGELRASRDEVEQKNVELEHMA
                     THDPLTGCLTRRALFDRMMRAREDARRNGKPLSCLAVDIDQFKSVNDRFGHVLGDRVV
                     EEVGKTLAASLRGTDIVGRYGGDEFFVGMPGCDAEHALQLAEKVRRSIKQHCSTNFPE
                     LRVTVSIGVAAMTGGEESVAALIEQADQALYAAKSSGRNRVKIAA"
BASE COUNT          400 a          888 c          884 g          347 t
ORIGIN      
        1 gcaccagacg cggcgcgagc gacccgtcaa ccagctcgtc acatggtgat ctcctctcga
       61 agccttgcca ggcggctggc ggccatcacc ctggcagccg gcatctgcgc gggctgcccg
      121 gggtttgccg cccagccggc ggggcggggc gccggggatc tcgcctgcgg cccggtgttc
      181 gacagcaaag gcgcggtcgt cctgggacac gggaccgggg attcggtcaa gagcgcctcg
      241 aagcggaagt gccccggtgt ctccacgcca cgactggcgc cacccaaggc gcggacgccc
      301 gtgatcatgg tcggcgcgca ggagcaggcc gagagcgagg atgagctcgc cgtactcgac
      361 accgatgtgg gcagggtcag cgccgcagtg gtgaccgggg gcgccctgtt ctggctgctg
      421 caatccagca tctggacctc cctgctgatc ctggggatac ccctgtggcg gcacgtcgat
      481 ctgctgccga tcgtggaccg tgcgtccgac ccggctccgg cgagcgtgcc gcccaagcgc
      541 gacgcctccg aagagcaggc ggtcgcggtc atgctcgatg cgcagcgccg gcggcgccgc
      601 ggaaggcgga ccacaccgtg aagcgcatcc ggcccagcgc ccagatcacc ctcgggctgg
      661 tgctgctgac caccgcggtc ctgctgctgg tggacctgct gttcaacgtg ttcccggacc
      721 cggacgccca gctcatgcgc gtgcgcggcg cgcacgccga gagcctggcg acacaggtct
      781 cggtgctgtt gcagcgtggc gacgagcaca gcctgtcgct ggcgctggcc cgcgtgcgcg
      841 cgcgcgactc gagcatccgc tcgctggccg tgcggcgcgt cgggcacggg ttgctggcgc
      901 gatcgggcga tcacgagcgg gcgtgggccg agccgcgcca cgcgcgctcg gcgctgacgc
      961 atctgctggt gccgttgacg cagagcggca cgcgctgggg cagcttcgag atgaccttct
     1021 tccccgacga cagcggcccg ttgcagcgcg tgttcgccca cccgctgtgg gtgacgttgc
     1081 tcagcaccgc gctcatcggc gcgctggtgt actgggtgta catccgccgc gcgctgatgc
     1141 acctggatcc caccgcggtg atcccggagc gcgtgcggct cgccttcgac atcatgaccg
     1201 agggcgtcgc ggtgctggac aaccgcggcc gcctgctgct ggccaacagc gcgttccgcg
     1261 cgctgcacgg caaggacggt ccggatctgg tcggcgcgcg cctgtcggac ttgccgtggc
     1321 tcgcggccag cctgcccccg aacgccgccg accatccctg gagccgcgcc atgcatcagg
     1381 ccacgccggt cagggatgtg gcgatcgagg tggcgccggc ggcggacgtg cgcagaaagc
     1441 tggcggtcaa ctgcgcgccg atctccgatg cgcgcgggct ggtgcgcggc tgcatcgcca
     1501 ccttcgccga cctcacggcg ctgcacttcg ccaacgagcg gctgaccgag acgctgggag
     1561 agctgcgtgc atcgcgcgac gaagtcgagc agaagaacgt cgagctcgag cacatggcca
     1621 cgcacgatcc gctgaccggc tgcctgaccc gccgggcgct gttcgaccgc atgatgcgcg
     1681 cgcgggaaga cgcgcgacgc aacggcaagc cgctcagctg cctggcggtg gacatcgacc
     1741 agttcaagtc ggtcaacgac cgcttcgggc acgttctcgg cgaccgagtg gtcgaggagg
     1801 tcggcaagac cctggcggca tccctgcgcg gcacggacat cgtcggccgc tacgggggcg
     1861 acgagttctt cgtcggcatg cccgggtgcg atgccgagca cgcgctgcaa ctggccgaga
     1921 aggtccggcg cagcatcaag cagcactgca gcacgaactt cccggaactg cgcgtcaccg
     1981 tgagcatcgg cgtggcggcg atgaccggtg gagaggaaag cgtggctgcg ctcatcgagc
     2041 aggccgacca ggcactctac gcggccaagt ccagcggccg caaccgcgtg aagatcgccg
     2101 cgtagccgcg gcagcatcgc tcaccctgcc ctctcccgca agcgggagag ggggcgcgct
     2161 gccgatgctt caaggcgcac gccgccagtc ccctagtctt cacggccgcc aggaacgggc
     2221 taagatggat gccggcggtc gaagcatccc gtcgccttcg cccaaaatcg aaagcaaggc
     2281 agcccgaatc cgaaagcacg acccccgaat tcgaaagcac gacccccgaa tttgaaagca
     2341 agacccccga attcgaaagc aagacccgga gtgccaggcc tcgccacggc ggctagagtg
     2401 cgacagtcat cgaactgtcg gaggagtcga acatgagcac ctcagcgcac aacgcccggc
     2461 tcgcggccgc aaccgccggc gactcccgct ggaccgccgt cgtcgcgcgc gatgcggcg
//
LOCUS       DASPGE010000130         9334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1560235, whole genome shotgun sequence.
ACCESSION   DASPGE010000130 DASPGE010000000
VERSION     DASPGE010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560235"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VD791_04095"
     CDS             complement(<1..441)
                     /locus_tag="VD791_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HXV07184.1"
                     /translation="MGWAILVHGGAGEWPAQDEAAAIAGVGLAVSHAAQGLAAGDSAL
                     DAAVAAVVELEDNALFNAGTGAVLNRDGDAELDASVMAGHDLRCGAVAALRRVRNPVL
                     VARRVMEYTAHVMLAGEGALRFARSEGFEDYDPITQRARDAWLRR"
     gene            481..1848
                     /locus_tag="VD791_04100"
     CDS             481..1848
                     /locus_tag="VD791_04100"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXV07185.1"
                     /translation="MRDRVAAAALSRLQRSLRRELWLGIGLAALLAGLAFALTLHFVE
                     PAPPRHVVLSTGPADSGYHAFALRYREILARDGVTLALRTSQGSLDNVGRLMDDATDV
                     DAGFLQGGTSFAVNAPGLVSLGSLYYEPLWVFYRGREIDDLTGLRGRKIAIGPDFSGV
                     RTLALQLLAVNDVVLPPTELLPLDGDDAANALVAQRVDAAMFVAPGQSPTVERLIQEP
                     GVRLLSFERGEAYTRRFPFLTRLTLPRGTFDLARNVPRGDIVLIAPTANLLVRSHLHP
                     ALAYLFLRAATQLHGEAGLFNRRGEFPNTKDADFPLSAEAERYYASGTPFLQRYLPYW
                     AANLVDRLWVMLLPVLAVVIPLARLLPPLYSWRMRSRIYRWYARLKEIELQLERATSQ
                     DELNEMLSGLTDIEAAVNHIATPLAYSANLYSFRQHIDLVRGRVLQRLKGDRRVVPEK
                     RDREA"
     gene            complement(1897..3855)
                     /locus_tag="VD791_04105"
     CDS             complement(1897..3855)
                     /locus_tag="VD791_04105"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HXV07186.1"
                     /translation="MERIFSAEGPLARAIPAYKSRAQQVEMARAVARAIEARGVLIAE
                     AGTGTGKTFAYLAPALLSGGKVIVSTGTRTLQDQLFARDVPTVRQALRAPVTVALLKG
                     RANYICHYHLERNLADGRFPAREDARHIRAIARFARTSKTGDRAGVSDVPEDSSVWPF
                     ATSTRENCLGGECPRYDECFVMEARRQALAADLVVVNHHLFFADLALRDEGVAELLPA
                     CNTVIFDEAHQLPDTATLFFGESLSTAQLLELARDTRMEAVRAAQDFQPLPDAAGELD
                     RAARDLRIAAGNETGRQPAREFETRREFMLRLDALGEKLAALGALLAAQAERSEDLQH
                     CAQRCHELAARLQRWRSEAPDESVRWVEVFQYALQLNTTPLAVADIFRKQVTGGARAW
                     IFTSATLSVKGDFSHYQQQMGLEQAQAATWDSPFDYSHQALLYVPAHMPGPNSAGYTR
                     AVVEAALPVLAASRGRAFLLFTSLRAMREAHALLAERFAETGLDFPLLLQGEGSRSEL
                     LERFRRLGNAVLVASQSFWEGVDVRGEALSLVLIDRLPFSPPDDPVLAARIERISAQG
                     RNAFMEYQLPEAVIALKQGAGRLIRDETDRGVLMICDARLVDRPYGRRIWQSLPPMRR
                     TRQVEEVVEFFAKGVTEKRDRNGVTGTA"
     gene            4005..4205
                     /locus_tag="VD791_04110"
     CDS             4005..4205
                     /locus_tag="VD791_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HXV07187.1"
                     /translation="MQRLLIVLGVILLAVGLLWPWLSKLGLGRLPGDIRIERDGFGFY
                     FPLTTGLIVSIVISVILWLLRK"
     gene            4219..4812
                     /locus_tag="VD791_04115"
     CDS             4219..4812
                     /locus_tag="VD791_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV07188.1"
                     /translation="MADSSLPDFWDTRYQTNVMPWDAGRVPDALRRHLAAFPAAGRVL
                     VPGCGSAYEVRCLAEHGFDVLAIDFSVAAVARARKELGPFADRVRQADFFDFDLGGRP
                     FDVIYERAFLCALPQRLWPRYAERSAQVLAPGGRIAGFFYFDDSPRGPPFGTGDAQLQ
                     ALLSPWFDRIEDEPVMDSLPVFQGKERWQLWVRRPRP"
     gene            complement(4793..5431)
                     /locus_tag="VD791_04120"
     CDS             complement(4793..5431)
                     /locus_tag="VD791_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HXV07189.1"
                     /translation="MIRPATGASARFHCGLLLAGIAVLVWSGYRPKDYFTWILEVAPA
                     IIAGVVLIALYSRFRFTDLVYALILAHAVILMVGGHYTYAEMPLFSWLRDEFGLARNY
                     YDRVGHVAQGFVPAMIAREVLLRNRVVNGRRWLFFVICCVALAISALYEFLEWWVAVA
                     SGTAAEAFLATQGDVWDTQWDMFLALCGAVAAQLLLNGWHDAQLARLRGAVV"
     gene            complement(5428..6282)
                     /locus_tag="VD791_04125"
     CDS             complement(5428..6282)
                     /locus_tag="VD791_04125"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HXV07190.1"
                     /translation="MFRAIGTALLLLVGLAGAAHAQQTALEVITLNYRNAEEVMPILR
                     PLLAPQGTITGLNNRLVVRTTPANLAELKQVLAAIDAAPRRLLISVRQDADLDRARRG
                     GQISGQADIGDNATVRVPGRPTPPGATVRADGVRARVYGDESTASDRLSQQAQVIEGG
                     RAFIRVGQSVPVHSRGVIDTPDGRRVVEATAFREADTGFYVAPRVTGDRVTLEISTAA
                     DTLQSGQRGAVRVQRVQTVVTGRLGEWIELGAVDQQSAQVDSEILGRSRDARRDQRRV
                     LVKVDEIR"
     gene            complement(6282..7085)
                     /locus_tag="VD791_04130"
     CDS             complement(6282..7085)
                     /locus_tag="VD791_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HXV07191.1"
                     /translation="MLFGALRGWRRERILRQSSLDERLWRDTVRRLHFVRHLPQQDLQ
                     RLRDLVVLFLHDKHFATAHGLELTARMQVWVAAQACMLILELGLDYYRGWSGIILYPE
                     QFVPRHQHTDELGVVHQGDEPYAGEAWLGGPVVLSWADVQGGDYADGVNVVIHEFAHK
                     LDMLNGEANGFPPLHAGMSRQLWADTFRAAYEDFCVRVDADEDTLIDPYAGESPGEFF
                     AVLSEAFFETPDVVRDEYSEVYAQLVQFYRQDPARWLARHGRRSRAARS"
     gene            complement(7085..8626)
                     /gene="rmuC"
                     /locus_tag="VD791_04135"
     CDS             complement(7085..8626)
                     /gene="rmuC"
                     /locus_tag="VD791_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HXV07192.1"
                     /translation="MDPLILMIVTAALSAVVLVVLVWVGVRLGALRRRQDNVPGTVGE
                     ALERKHLEMVKDLNNGLNTLGDRLGERLNESAERLRGAVSQDLKQTRDAMLVLQLSQT
                     EELSSNREAMTERLVNLSSGLQAKHDELRAELLTRVMERLGEQGRASHEMIQNTLHGI
                     GQQIGASIEGLSRTTDARLEQISGKVNERLEEGFKKTNDTFVSVMARLATIDEAQRKI
                     DGLTSNVVSLQELLGDKRSRGAFGEIQLEALVRNLLPPSAYAFQHDLGNGTRVDCVLM
                     LPEPTGMVAVDAKFPLENYHRMFDWDLAEGDRASALRQFKADVRRHVDDICERYIVAG
                     ETADGAVMFVPAEAVFAEIHAHHPETVDYAMQRRVWIVSPTTMMAVLNTARAVLKDVE
                     TRRQVHIIKDELSKLGKEFGRFDQRMHKLAEHIRQAHQDAQDVRITSEKISRRFSQIE
                     RVELEQSASLAQLERALLDQASPDQAPSDQAPPDQAPSDRAPPDRAPVDQAEPDAPLP
                     RSEAG"
     gene            complement(8695..>9334)
                     /locus_tag="VD791_04140"
     CDS             complement(8695..>9334)
                     /locus_tag="VD791_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HXV07193.1"
                     /translation="RSEPYELLTVDLDDPHQGAAMEALGARRGELIDMQSDGKGRTRL
                     EYRIPARGLLGFQTEFLNLTRGTGIMSHVFDDWGAVKPDMPERRNGVLISAEEGELVP
                     YALWKLEPRGRMFVSPGEPVYEGMIIGVHSRDNDLVVNPVREKKLTNIRAAGKDENVV
                     LTPPIRLTLESAIEFIADDELVEITPKSIRLRKRHLKPHERKRAGRTEGAPA"
BASE COUNT         1350 a         3419 c         3081 g         1484 t
ORIGIN      
        1 gcggcgcagc caggcgtcgc gcgctcgctg cgtgatcggg tcgtagtcct cgaagccttc
       61 gctgcgcgca aagcgcagcg ctccctcgcc cgccagcatc acgtgcgcgg tgtattccat
      121 gacccggcgc gcgacgagca ccggattgcg cacgcgccgc agcgccgcca ccgcgccaca
      181 acgcagatcg tgcccggcca tgaccgaggc gtccagctcc gcgtcgccgt cgcggttgag
      241 caccgcgccg gtgccggcgt tgaacagcgc gttgtcctcc agctcgacca cggccgccac
      301 cgccgcatcg agcgcgctgt ctcccgccgc aagaccctgc gccgcgtggc tcacggccag
      361 gccgacgccc gctatcgccg ccgcttcgtc ctgcgcaggc cattcgccgg cgccgccgtg
      421 aaccagtatc gcccatccca tgatcgcctc tcgctcccgc ccctataatg cagcatgcgc
      481 atgcgtgacc gtgtcgccgc ggccgccctg tcgcggctgc agcgcagcct gcgccgggaa
      541 ctctggctcg ggatcggcct ggccgcgctg ctggccggcc tggccttcgc cctcacgctg
      601 cacttcgtcg agcccgcacc cccgcggcac gtggtgctca gcaccggccc cgccgacagc
      661 ggctatcatg ccttcgcgct gcgctaccgc gagatcctgg ctcgcgatgg cgtcacgctc
      721 gcgttgcgca cctcgcaagg cagcctggac aacgtcggcc gcctgatgga cgacgccacg
      781 gacgtggacg ccggcttcct gcagggcggc acctcgttcg ccgtcaacgc acccggactg
      841 gtgtcgctgg gcagcttgta ctacgagccg ctgtgggtgt tctaccgcgg ccgcgagatc
      901 gacgatctga ccggcctgcg gggcaggaag atcgcgatcg gacccgactt cagcggcgtg
      961 cgcacgctgg cgctgcagct gctcgccgtg aacgacgtcg tgctgccgcc caccgagctg
     1021 ctgccgctcg acggagatga tgcggcaaac gcgctggtcg cgcagcgggt ggacgcggcg
     1081 atgttcgtgg cgcccgggca gtcccccacg gtcgagcgcc tgatccagga gccgggcgtg
     1141 cgcctgctga gcttcgagcg cggggaggcc tatacgcgcc gcttcccctt cctcacccgg
     1201 ctgacgctgc cgcggggcac attcgacctg gcgcgcaatg ttccgcgcgg ggatatcgtc
     1261 ctgatcgcgc ccaccgccaa tctgctggtg cgcagccacc tgcatccggc actcgcctac
     1321 ctgttcctgc gagcggccac gcaactgcac ggggaagccg ggctgttcaa ccggcgcggc
     1381 gagttcccca acaccaagga cgcggacttt ccgctcagcg cggaagcgga gcgctactac
     1441 gcatcgggca cgccgttcct gcagcgctac ctgccttact gggccgccaa cctggtggac
     1501 cggctctggg tgatgctgct gccggtgctg gcggtggtca taccgctggc gcggctgctg
     1561 ccgccgctct acagctggcg tatgcgctcg cgcatctacc gctggtacgc gcgcctcaag
     1621 gagatcgagc tgcagctcga gcgcgccacc agccaggacg agctgaacga gatgctgtcc
     1681 ggactgaccg acatcgaagc cgcggtcaac cacatcgcga ccccgctcgc ctactccgcc
     1741 aacctgtaca gcttccgcca gcatatcgat ctggtgcgcg ggagagtgct gcaacggctc
     1801 aagggggatc ggcgggtagt tccagaaaag cgtgaccgag aagcgtgaca aaaccagttg
     1861 gtagagtcga gaggcggttc tcgccacgcc tttcggtcac gccgttccgg tcacgccgtt
     1921 ccggtcacgc ttctcagtca cgcctttcgc aaagaactcc acaacctcct ccacctgccg
     1981 cgtgcgccgc atcggcggca ggctctgcca gatccggcgg ccgtacggcc ggtccaccag
     2041 ccgcgcatcg cagatcatca gcacgccgcg gtcggtctcg tcgcggatca gccgtcccgc
     2101 cccctgcttg agcgcgatca ccgcttcggg gagctgatac tccatgaagg cgttacggcc
     2161 ttgcgcgctg atgcgctcga tgcgcgcggc cagcaccggg tcgtcgggcg gcgagaacgg
     2221 caggcggtcg atcaacacca gcgacagcgc ttcgccgcgc acgtccacgc cttcccagaa
     2281 cgactggctc gccaccagca ccgcgttgcc cagacgccgg aagcgctcga gcagctcgct
     2341 gcgcgagccc tctccctgca gcagcaaagg gaagtcgagc cccgtttcgg cgaagcgctc
     2401 ggcgagcagc gcatgcgcct cgcgcatcgc gcgcaggctg gtgaacagca gaaaggcgcg
     2461 cccgcggctg gcggccagca ccggcaacgc cgcctcaacc accgcacggg tgtaacccgc
     2521 gctgttcggc cccggcatgt gcgcaggcac gtacagcagc gcctggtgcg aatagtcgaa
     2581 cggactgtcc caggtcgccg cctgcgcctg ctccagcccc atctgctgct gatagtggct
     2641 gaagtcgccc ttcaccgaga gcgtggcgga ggtgaagatc caggcgcgcg cgccgcccgt
     2701 gacctgcttg cggaagatgt cggccaccgc cagcggcgtg gtgttgagct gcaacgcgta
     2761 ctggaacacc tcgacccagc gcacgctctc gtccggcgcc tcgctgcgcc agcgctgcag
     2821 cctggcggcc agctcgtggc agcgctgcgc gcaatgctgc agatcctcgc tgcgctcggc
     2881 ctgtgccgcc agcagtgcgc cgagcgcggc gagcttctcg ccgagcgcgt ccaggcgcag
     2941 catgaattcg cgcctggtct cgaactcgcg agccggctgc cggccggttt cgttgccggc
     3001 ggcgatccgc agatcgcgcg ccgcacggtc gagctcgccg gcggcgtccg gcagtggctg
     3061 gaaatcctgc gcggcgcgca ccgcctccat ccgcgtatcg cgcgccagct cgaggagctg
     3121 cgcggtggac aggctctcgc cgaagaacag cgtggcggtg tccggaagct gatgcgcctc
     3181 gtcgaagatc accgtgttgc atgccggcag cagctccgcc acgccctcgt cgcgcagcgc
     3241 cagatcggcg aagaacaggt ggtggttcac caccaccagg tcggccgcca gcgcctgcct
     3301 gcgcgcctcc atcacgaagc actcgtcgta ccgcgggcac tcgccgccca ggcagttctc
     3361 gcgcgtggag gtggcgaacg gccacacgct ggagtcctcc ggcacgtcgc tcacgcccgc
     3421 ccggtcgccg gtcttgctgg tgcgtgcgaa ccgggcgatg gcccggatgt gccgcgcgtc
     3481 ctcgcgcgcc gggaaacgac cgtcggcgag gttgcgctcg aggtggtaat ggcagatgta
     3541 attggcgcgc cccttgagca gggccacggt gaccggagcg cgcagcgcct ggcggacggt
     3601 cgggacgtcg cgcgcgaaca gttgatcctg caaggtcctg gtgccggtcg agacgatgac
     3661 cttgccgccc gacagcagcg ccggcgccag ataggcgaag gtcttgccgg tgccggtgcc
     3721 ggcctccgcg atcagcacgc cgcgcgcctc gatcgcccgc gccaccgcgc gcgccatctc
     3781 cacctgctgc gcgcgcgact tgtaggcggg aatcgcacgc gccagcggcc cctccgccga
     3841 aaagatccgc tccatcgcat ccatgcgtgg cgggcattgt atcggcccgt cacgcccggc
     3901 gcgagcccgc atcccgccgg tgctatcctt gcgtaggctc cctctgcctt gcgggagctg
     3961 gggtgagggg tacgttcgaa tcggccgtta cagtccgcga gctcatgcag cgcctgctca
     4021 tcgttctggg agtgatcctg ctcgccgtgg gcctgctctg gccctggctg agcaagctcg
     4081 ggctggggcg cctgcccggg gacatccgca tcgagcgcga cggcttcggc ttctacttcc
     4141 ctctcaccac cggcctgatc gtctccatcg tcatcagcgt gatcctgtgg ctcctgcgga
     4201 agtagacacc gattcccgat ggccgacagc tcgctccccg acttctggga cacccgctac
     4261 caaaccaacg tcatgccctg ggatgcgggc cgcgtgccgg acgcgctgcg ccggcatctc
     4321 gccgccttcc ccgccgcggg gcgggtactg gtgcccggct gcggctcggc ttacgaagtc
     4381 cgctgcctgg cggagcacgg attcgacgtg ctcgccatcg acttcagcgt cgcggcggtg
     4441 gcgcgtgccc ggaaggagct cggccctttc gccgatcggg tacggcaggc ggatttcttc
     4501 gacttcgatc tcggcggccg gccattcgac gtgatctacg agcgcgcctt cctgtgcgcg
     4561 ctgccgcagc gattgtggcc gcgctacgcg gaacgcagcg ctcaggtgct agcacccggc
     4621 ggacggatcg ccggcttctt ctacttcgac gacagcccgc gcgggccgcc cttcggcacc
     4681 ggcgatgcgc agctccaggc gctgctctcc ccgtggttcg atcgcatcga ggacgagccc
     4741 gtgatggatt ccctccccgt gttccagggg aaggagcgct ggcagctatg ggtcagacga
     4801 ccgcgcccct gagccgggcg agctgggcat cgtgccagcc attcaacagc agctgcgccg
     4861 ccaccgcccc gcacagcgcg aggaacatgt cccactgcgt gtcccacacg tcgccctggg
     4921 tggcgaggaa cgcctcggcc gcggtgccgg aggcgaccgc gacccaccat tcgagaaatt
     4981 catacagcgc cgagatcgcc agcgccacgc agcagatcac gaagaacagc cagcgccgcc
     5041 cgttcaccac gcgattgcgc agcaggacct cgcgcgcgat catggccggg acgaaaccct
     5101 gagcgacgtg ccccacgcga tcgtagtagt tgcgcgccag cccgaactcg tcgcgcagcc
     5161 agctgaacag cggcatctcg gcgtaggtgt agtggccgcc gaccatcagg atgacggcgt
     5221 gcgccaggat cagcgcgtag accaggtcgg tgaagcggaa ccgcgaatac agcgcgatca
     5281 gcaccacgcc ggcgatgatc gccggcgcga cctcgaggat ccaggtgaag taatctttag
     5341 gccggtaacc ggaccagacc agcacggcga tgccggccag cagcagcccg cagtggaacc
     5401 gtgcgctggc gcccgtcgcc ggacggatca tcggatctcg tcgaccttca ccagcacgcg
     5461 ccgctgatcg cgccgcgcgt cgcggctgcg ccccaggatc tcgctgtcga cctgtgccga
     5521 ctgctggtcg accgcgccga gctcgatcca ttcgcccagg cgcccggtca ccacggtctg
     5581 cacgcgctgc acccggacgg cgccgcgctg gccggattgt agcgtgtcgg cggcagtcga
     5641 gatctccaag gtcacgcgat cgcccgtcac ccgcggcgcc acgtagaagc cggtatccgc
     5701 ttcgcggaac gcggtcgcct cgacgacccg cctgccgtcc ggcgtgtcga tcacgccgcg
     5761 gctgtgcacc ggcaccgact gtcccacccg gatgaaggcg cgcccgccct cgatcacctg
     5821 cgcctgttga gacagccggt cgctcgctgt cgattcgtcg ccgtacaccc tggcgcgcac
     5881 gccgtcggcc cgcaccgtcg caccgggcgg cgtcgggcgt ccgggcacgc gcacggtcgc
     5941 gttgtcgccg atgtcggcct gaccggagat ctggccgccg cggcgcgcgc ggtccagatc
     6001 cgcgtcctgg cgcaccgaga tcaacagccg gcgcggcgcc gcgtcgatcg ccgccagcac
     6061 ctgcttgagc tccgcgagat tggccggcgt ggtgcgcacc acgagccggt tgttcaagcc
     6121 ggtgatcgtc ccttgcggcg cgagcagcgg cctgaggata ggcatcacct cctcggcatt
     6181 gcggtagttg agggtgatga cctccagggc ggtctgctgc gcatgggcgg cacccgccag
     6241 accgacgagg agcagcagtg cggtgccgat cgcgcggaac atcagctgcg agccgccctg
     6301 ctgcgccggc cgtgccgcgc cagccagcgc gccggatcct gccgatagaa ctgcacaagc
     6361 tgcgcgtaga cctcggaata ttcgtcgcgc accacgtccg gggtctcgaa gaacgcctcg
     6421 gacaggaccg cgaagaattc tcccgggctc tcgccggcgt acgggtcgat gagcgtgtct
     6481 tcgtccgcgt cgacgcgcac gcagaagtcc tcgtacgcgg cgcgaaaggt gtcggcccag
     6541 agttgccggc tcatccccgc atgcagcggc ggaaacccgt tggcctcgcc gttgagcatg
     6601 tccagcttgt gcgcgaactc gtggatcacc acattgaccc cgtcggcgta gtcgccgccc
     6661 tgcacgtcgg cccaggacag caccaccggc ccgcccagcc aggcctcccc ggcatagggc
     6721 tcgtcgccct ggtgtaccac ccccagctcg tcggtgtgct gatggcgcgg cacgaattgc
     6781 tccgggtaca ggatgatgcc gctccagccg cgatagtagt cgagccccag ctcgagaatc
     6841 agcatgcagg cctgcgccgc cacccacacc tgcatcctgg cggtcagctc caggccgtgc
     6901 gcggtggcga agtgcttgtc gtgcaggaac agcaccacca ggtcccgcag ccgctgcagg
     6961 tcctgctgcg gcagatggcg cacgaaatgc aggcggcgca cggtgtcgcg ccacaggcgc
     7021 tcgtccaggc tcgactggcg caggatgcgc tcccgccgcc agccgcgcaa cgccccgaac
     7081 agcatcagcc cgcctccgac ctcggcagcg gcgcgtcggg ctccgcctga tccacgggcg
     7141 ctcgatctgg cggcgctcga tctgacggcg cttgatctgg cggcgcttga tccgagggtg
     7201 cttgatccgg ggacgcctgg tcgagcaggg cgcgctcgag ctgcgccagg ctggccgact
     7261 gctccagctc gacgcgctcg atctgcgaga agcggcgcga gatcttctcg ctggtgatgc
     7321 gcacgtcctg cgcgtcctgg tgcgcctggc ggatatgctc ggcgagcttg tgcatgcgct
     7381 gatcgaagcg gccgaactcc ttgccgagct tggacagctc gtccttgatg atgtgcacct
     7441 gccggcgcgt ctccacgtcc ttgagcacgg cgcgcgcggt gttgagcacc gccatcatgg
     7501 tggtgggcga cacgatccag actcggcgtt gcatcgcgta gtctacggtc tccgggtggt
     7561 gcgcatgaat ctccgcgaac accgcctcgg ccggcacgaa catgacggcg ccgtcggcgg
     7621 tctcgccggc gacgatatag cgctcgcaga tgtcgtccac gtgccggcgc acgtccgcct
     7681 tgaactggcg cagcgcgctg gcccggtcgc cttccgccag gtcccagtcg aacatgcggt
     7741 gatagttctc cagcgggaac ttggcgtcca ccgccaccat cccggtgggc tcgggcagca
     7801 tcagcacgca gtccacgcgc gtgccgttgc cgaggtcgtg ctggaaagcg taggccgaag
     7861 gcggcaggag attgcgcacc agggcctcga gctggatctc gccgaacgcg ccgcgcgagc
     7921 gcttgtcgcc cagcagctcc tgcaggctga ccacgttgct ggtgagcccg tcgatcttgc
     7981 gctgggcttc gtcgatggtg gccagccggg ccatcacgct gacgaaggtg tcgttggttt
     8041 tcttgaagcc ctcctccagg cgctcgttca ccttgccgct gatctgctcc agccgggcgt
     8101 cggtggtgcg cgacagcccc tcgatcgacg cgccgatctg ctggccgata ccgtgcagcg
     8161 tgttctggat catctcgtgg ctggccctgc cctgctcgcc caggcgctcc atcacgcgcg
     8221 tgagcagctc ggcgcgcagc tcgtcgtgct tggcctgcag cccggaggac aggttcacca
     8281 ggcgctcggt catcgcctcg cggttgctcg acagctcctc ggtctgcgac agctgcagca
     8341 ccagcatcgc atcgcgcgtc tgcttcaggt cctgagacac tgcgccgcgc agccgctccg
     8401 cgctctcgtt caggcgctcg ccgaggcggt cgccgagcgt gttcaggccg ttattgaggt
     8461 ccttgaccat ctccaggtgc ttgcgctcca gcgcctcccc gacggtcccg ggcacgttgt
     8521 cctggcgccg gcgcagcgcc ccgaggcgca cgccgaccca gacgagcacc accagcacca
     8581 ccgcgctcag cgctgcggtg acgatcatca gaatgagggg atccatgccc gcagtatacc
     8641 gcccgtcggg gcccgcaacg gctacgccgc tgcccccgct aacgccgctt tcctctacgc
     8701 cggcgcccct tcggtgcgcc ccgcccgctt gcgctcgtgc ggcttcaggt gccgcttgcg
     8761 cagccggatc gacttcggcg tgatctccac cagctcgtcg tcggcgatga actcgatggc
     8821 cgattccagc gtgaggcgga tgggcggggt cagcaccacg ttctcgtcct tgccggcggc
     8881 gcggatgttg gtgagcttct tctcgcgcac cgggttcacc accagatcgt tgtcgcggct
     8941 gtggacgccg atgatcatgc cctcgtacac cggctcgccc gggctcacga acatgcgccc
     9001 gcgcggctcg agcttccaca acgcataggg caccagctcg ccctcctcgg ccgagatcag
     9061 cacgccgttg cgccgctcgg gcatgtccgg cttgaccgcg ccccagtcgt cgaacacgtg
     9121 gctcatgatg ccggtgccgc gcgtcaggtt gaggaactcg gtctggaagc cgagcaggcc
     9181 gcgcgccggg atgcggtatt ccaggcgcgt gcgccccttg ccgtcggact gcatgtcgat
     9241 cagctcgccg cggcgtgcgc ccagcgcttc catcgccgcg ccctgatgcg ggtcgtcgag
     9301 gtccacggtg agcagctcgt agggctcgga tctc
//
LOCUS       DASPGE010000131         9835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1210181, whole genome shotgun sequence.
ACCESSION   DASPGE010000131 DASPGE010000000
VERSION     DASPGE010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9835
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210181"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VD791_04145"
     CDS             complement(<1..667)
                     /locus_tag="VD791_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV07194.1"
                     /translation="MGFLQDKKILVTGMLSTRSIAYGIARAMAREGARLAFTYQGEGV
                     KERVLALAQEFGTDLVFACDVTSDAEIDALFAALRGRWDGLDGLVHSIAFAPREALAG
                     DYLESVSREAFRVAHDISSYSLAALAKGALPMMQGRRAAVVTLSYLGAERSMPNYNVM
                     GVAKASLEANVRYLALSLGPKGIRVNAISAGPIKTLAAAGIGGFGKILAYNEKIAPLR
                     RNVT"
     gene            805..2970
                     /locus_tag="VD791_04150"
     CDS             805..2970
                     /locus_tag="VD791_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV07195.1"
                     /translation="MRAPVLALALLAGGCGHPWNNPYPAAERGANILYTSFAERPKHL
                     DPVQSYSENEYALIANIYMPPLQYHYFKRPYELIPLAAETIPSARYYDSAGRALPDTA
                     AAQQIAYTVYEIRIRPGFKYQPHPAFALDQSGQPRYRDLSPEFVRGIHELSDFGHTGT
                     RELIAEDYVYQLKRLAHPHLHSPILGLMTEYIIGLKEYAAALKELAQRTPAGQWIDLR
                     ALDIDGVSVVDRYTYRIEIKGKYPQFVYWLAMPFFAPIPAEADRFYAQPGLAERNINF
                     DWYPVGTGPYMLTVNNPNRQMVLERNPNFPGEPYPGEGEPQDAALGLLADAGKPMPLV
                     DRVVFSLEKETIPYWNKFLQGYYDASGITSDNFDQVIQVSGTGDAQLTPKMQRQGIKL
                     DTSVATSIMYIGFNMLDPVVGGQSERARKLRQAISIALDQEDFISIFLNGRGIAAQGP
                     IPPGIFGYRDGEAGINRYLYDWVDGEPRRKSVEHAKGLLAEAGYPNGIDRDTGKPLTI
                     YFDSTLTGVGAKARSDWLIKQLQRIDLQLVVRTTDYNRFQEKMRKGTAQLYYWGWNAD
                     YPDPENFLFLFHGPQGKAKFSGENASNFASTEFDLLFERMRAMENGPERQALVDRLVE
                     ILRREAPWAGGFFPKDFALAHQWVHNRKANKMANNGLKYQRLDPALRERLRQQWNRPV
                     LWPIGLVALALAAGLLPAVSAYRRRERAAAVAGSGEARA"
     gene            2973..3950
                     /locus_tag="VD791_04155"
     CDS             2973..3950
                     /locus_tag="VD791_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV07196.1"
                     /translation="MFAYIVRRVLYAVPILIGVNLITFALFFFVNSPDDIARMQLGQK
                     RVTPEAIQKWKVERGYDLPMLYNPGAQGGQKLTDTIFYRKSVSLFWFDFGRAEDGRDI
                     GQEIRTRMWPSLAIAVPMFLVGLACYVSVALVLAFFRATYVDFAGVVLCVAAMSISGL
                     FYIIGGQYLVSKLWNLVPISGYGQGIDTWRFVILPAAIGVIGGIGSSARLYRTIFLEE
                     INRDYVRTARAKGLTEGRVLFGHVLRNGLIPILTGVVVAIPGLFIGSLVLESFFGIPG
                     LGSYTIDAINAQDFAVVRAMVFLGAVLYIAGLILTDISYTLADPRVRLQ"
     gene            3950..5419
                     /locus_tag="VD791_04160"
     CDS             3950..5419
                     /locus_tag="VD791_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV07197.1"
                     /translation="MPYRYIMLWTDALVWLLVTCAIGFAWYVAQHEHLRAPWRKVAQS
                     RYGMSALVVLCVFVAIGLLDSIHYQERLETRQADGAANYSAEVLSLFDALAGRLREKP
                     ERTYSAPLTAYGFAKENVMRPDGTQAREFPRLQHGGAHLTDPARQLPLDVLRKTLAGA
                     GLAVALWIGLTMLLAAWLAARQRQPFGATLTEIIHGRTQIPWRDMLLTAGVVLLVACP
                     MGVLSAYYHVLGTDKVGHDVLYLTLKSIRTGLVIGTLTTLVLLPFALVLGLMAGYFRG
                     WIDDIIQYLYTTFSSIPGVLLIAAAVLAGQALMDRYADMFETVTQRADARLLFLCLIL
                     GITSWTGLCRLLRGEALKLREMDYVQAARAFGVPDWRIINRHLLPNVMHIVLIAIVMD
                     FSGLVLAEAVLSYVGVGVDPSMISFGTMINNARLELAREPMVWWSLAAAFVFMFLLVL
                     SANLFSDAVRDAFDPRIRTGWSLFLPYRSRPALAVAIRR"
     gene            5416..7488
                     /locus_tag="VD791_04165"
     CDS             5416..7488
                     /locus_tag="VD791_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV07198.1"
                     /translation="MSAPPLLEVADLRTWLHTANGVVRAVDGISFTVQRGETFALVGE
                     SGCGKSMTALSLMRLLPDAGRIVGGRALLEGTDLLALPEAAMREVRGARLGIIFQEPG
                     TSLNPVLTVGAQIEEVLQQHARLRGPAARRRALELLEQVGIPDAARRLDEYPFQMSGG
                     MKQRAMIAMALACNPGLLIADEPTTALDVTIQAQVLEVLRRLQREAGMSLLLITHDLG
                     VVAEMAHRVAVMYAGEIVECAPRERFFRAPAHPYSRKLFEALPGAAGRHRPLAVIPGS
                     VPRMDESFVGCRFADRCDRVMEVCRREPPPAVDLGDGQVVLCHLHAEARHEARGESRQ
                     ARGVNQVRGTRDEAGGEETSRLVPRASSLIDAPRTSSLLQVEDLKVHFPIRKGVFKRV
                     VGHVRAVDGVALEIPSHKTLALVGESGCGKTTVGKGLLHLLPLTGGRVLFEGEDLASL
                     SARRLRARRKDIQIIFQDPYASLNPRMRVMEIVEEGMAALGIGASEAERQRRVDRLLE
                     QVGLAPDMKLRYPHEFSGGQRQRIAIARALAVEPKLIVCDEPTSALDVSVQAQILNLL
                     KDLQTRLGLAYLFITHNISVVEFLAHEVAVMYLGRIVEKGTVEEVVGSPRHPYTQALL
                     SAVPNIDAATKREVIRLQGELPSPSNPPAGCHFHPRCPRAMPDCRETYPGEVALSGTH
                     SVRCLLYK"
     gene            complement(7600..9087)
                     /locus_tag="VD791_04170"
     CDS             complement(7600..9087)
                     /locus_tag="VD791_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXV07199.1"
                     /translation="MTLKNTLFFAAAAAAGAASLCLPGAVFAEAALFAAPIASSRAAS
                     AAPGATVAMPPVASDAVEVVPAVEAPHVNGTPAAQWNGQVVEPVSAEDLWERIRAGFA
                     MEEVDSPLVLRHEAWYLNRPEYVARMIDRSRRYLYHIVAEVEKRGMPMEIALLPMIES
                     AYNPTAYSRARASGMWQFIPSTGRKYGLQQTWWYDGRRDVLAATRAAMDYLEFLHTMF
                     GDWELALAAYNAGEGSVQRAIAKNRRARKSTDYRHLKLPRETRNYLPKLQAVKNIVSN
                     PELFKVTLEPLPNEPYFAVVTAPPHIDVEMAAKLAGVPEEEFRYLNPGHHRPVITPVA
                     NRQLLVPADKADLFQANLESNDQPLVTWQTYQLKKGETLDTVADKFDISLERLKEVNG
                     LAAGRRVRTGQMLLVPLEHDEAETNLDETYASKDFQAAADDYHQRTIYRVKSGDTLGS
                     IARRYRTSVSKLKAWNGLRGTTIRIGQRLTIWHYGAPAKQRVSKR"
     gene            9249..>9835
                     /locus_tag="VD791_04175"
     CDS             9249..>9835
                     /locus_tag="VD791_04175"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV07200.1"
                     /translation="MSNLMDHQLAPDWFETPLGTHLVACEQAYFDHETADVFGFNAIQ
                     IGLPRFDFLRANRMPLRCRVAPDGGVRVRADFEQLPVSSSCADLVLLPHVLEFSDNPH
                     QVLREVSRILMPEGHVIVSGFNPWSLWGARGFLGTDRGDFPWRGQFIALPRLRDWMAL
                     LGIEIAAGRMGAYVPPVRSEKWLRRVSFLEAAGER"
BASE COUNT         1485 a         3333 c         3369 g         1648 t
ORIGIN      
        1 tggtgacgtt gcgccggagc ggtgcgatct tctcgttgta ggcgaggatc ttcccgaagc
       61 cgccgatgcc ggccgccgcc agcgtcttga tcggcccggc ggagatcgcg ttgacgcgga
      121 tgcctttcgg ccccaggctc aacgccaggt agcgcacgtt ggcctcgagg ctcgccttgg
      181 ccacccccat cacgttgtag ttcggcatcg aacgctccgc gccgagatag ctcagcgtga
      241 ccaccgcggc gcgccggccc tgcatcatcg gcaacgcgcc cttcgccagc gcggcgaggc
      301 tgtagctgct gatgtcgtgc gccacgcgga acgcctcgcg gctcacactt tccaggtagt
      361 cgccggccag cgcctcgcgc ggggcgaacg cgatcgaatg caccaggcca tccaggccgt
      421 cccagcgccc gcgcagggcg gcgaacaggg cgtcgatctc cgcgtcgctg gtgacgtcgc
      481 aggcgaacac caggtcggtg ccgaactcct gcgcgagcgc gagcacgcgc tccttcacgc
      541 cctcgccctg ataggtgaag gccagccgtg cgccctcgcg cgccatcgcc ctggcgatgc
      601 cgtaggcgat cgagcgggtg ctcagcatgc cggtgaccag aatcttcttg tcctgcagaa
      661 agcccatgtc gatctcccgg gacgtcttcc aaggggcggg tggcgtgcgc aattataatc
      721 ggcacggcgc gggcgtcgcg ctgccgccgc ccgcctttgc cggccgggct ttcgcggtac
      781 acttgccggc ccatgtcgca tcccatgcgc gccccggtcc tcgccctcgc cctgctggcg
      841 ggcggctgcg gccacccctg gaacaatccc tatcccgccg ccgagcgcgg cgccaacatc
      901 ctgtacacct cgttcgccga gcgccccaag cacctcgacc cggtgcagtc gtactcggag
      961 aacgaatacg cactgatcgc caacatctac atgccgccgc tgcagtacca ctacttcaag
     1021 cggccctacg agctgattcc gctggccgcc gagacgatcc ccagcgcgcg ctactacgac
     1081 agcgccggcc gcgcgctccc ggacacggcg gcggcgcagc agatcgccta caccgtgtac
     1141 gagatccgca tccggcccgg cttcaagtac cagccgcatc cggctttcgc gctggaccag
     1201 agcgggcagc cgcggtaccg cgacctgtcg cccgagttcg tgcgcggcat ccacgagctg
     1261 agcgatttcg ggcacaccgg cacgcgcgag ctgatcgcgg aggactacgt gtatcagctc
     1321 aagcgcctgg cgcacccgca tctgcactcg ccgattctcg gcctgatgac cgagtacatc
     1381 atcggcctga aggagtacgc ggcggcgctc aaggagctgg cgcagcgcac gcccgcggga
     1441 cagtggatcg acctgcgtgc gctcgacatc gacggtgtga gcgtggtcga tcgctatacc
     1501 taccgcatcg agatcaaggg caagtacccg cagttcgtgt actggctggc gatgccgttc
     1561 ttcgcgccga ttccggcgga ggccgaccgc ttctacgcgc agcccgggct cgccgagcgc
     1621 aacatcaact tcgactggta tccggtgggc accgggccct acatgctcac ggtgaacaat
     1681 cccaaccggc agatggtgct cgagcgcaac ccgaacttcc ccggcgagcc ctaccccggc
     1741 gaaggcgagc cgcaggacgc cgcgctcggg ctgctggccg acgccggcaa gcccatgcct
     1801 ctggtggacc gcgtggtgtt cagcctggag aaggagacca tcccctactg gaacaagttt
     1861 ctgcagggct actacgacgc ctccggcatc acctcggaca acttcgatca ggtgatccag
     1921 gtgtcgggca ccggcgatgc gcagctcacg cccaagatgc agcgccaggg catcaagctg
     1981 gacacttcgg tggccacctc gatcatgtac atcggcttca acatgctcga cccggtggtc
     2041 ggcggccagt cggagcgcgc gcgcaagctg cgccaggcga tctcgatcgc gctcgaccag
     2101 gaggatttca tctcgatctt tctcaacggc cgcggcatcg ccgcccaggg accgatcccg
     2161 ccggggatct tcggctaccg cgacggcgag gcgggcatca accgctacct gtacgactgg
     2221 gtcgacggcg agccgcggcg caagtccgtg gagcacgcca agggcctgct ggccgaggcc
     2281 gggtacccca acggcatcga ccgggacacc ggcaagcccc tgacgatcta cttcgacagc
     2341 acgctcaccg gagtcggcgc caaggcgcgc agcgactggc tgatcaagca gctgcagcgg
     2401 atcgacctgc agctggtggt gcgcaccacc gactacaacc gcttccagga gaagatgcgc
     2461 aagggcacgg cgcagctcta ctactggggc tggaacgccg attacccgga cccggagaac
     2521 ttcctgttcc tgttccacgg gccgcaaggc aaggccaagt tcagcggcga gaacgcgtcg
     2581 aacttcgcca gcaccgagtt cgatcttctc ttcgagcgca tgcgcgcgat ggaaaacggc
     2641 cccgagcgcc aggcgctggt ggaccgactg gtcgagatcc tgcggcgcga agcgccctgg
     2701 gccggcggat tcttccccaa ggacttcgct ctggcccatc agtgggtcca caaccgcaag
     2761 gccaacaaga tggccaacaa cgggctgaag taccagcggc tggatccggc tctgcgcgaa
     2821 cgcttgcggc agcagtggaa ccggccggtg ctgtggccga tcggcctggt cgcgctggcg
     2881 ctcgccgccg ggctgttgcc ggccgtgtcg gcgtaccgcc ggcgcgaacg cgccgccgcc
     2941 gtggccggtt cgggagaggc gcgggcgtag cgatgttcgc ctacatcgtg cgccgggtgc
     3001 tgtacgccgt cccgatcctg atcggcgtga acctcatcac cttcgcgctg ttcttcttcg
     3061 tcaactcgcc cgacgacatc gcgcgcatgc agctcggcca gaagcgcgtg actcccgagg
     3121 cgatccagaa gtggaaggtc gagcgcggct acgacctgcc gatgctgtac aaccccgggg
     3181 cgcagggcgg ccagaagctg accgacacga tcttctaccg caagtcggtg agcctgttct
     3241 ggttcgactt cggccgcgcc gaggacggcc gcgacatcgg ccaagagatc cgcacgcgca
     3301 tgtggcccag cctggcgatc gccgtcccga tgttcctggt gggcctggct tgctacgtca
     3361 gcgtggcgct ggtgctcgcc tttttccgcg ccacctacgt ggacttcgcc ggggtggtgc
     3421 tgtgcgtggc ggcgatgtcg atctcggggc tgttctacat catcggcgga cagtacctgg
     3481 tgagcaagct gtggaacctg gtgccgatct ccggctacgg ccagggcatc gacacctggc
     3541 gcttcgtgat cttgcccgcg gcgatcggcg tgatcggcgg catcggctcg agcgcgcgcc
     3601 tgtaccgcac catcttcctg gaggagatca accgcgacta cgtgcgcacc gcgcgcgcca
     3661 agggcctgac cgaggggcgc gtgctgttcg gtcacgtgct gcgcaacggg cttattccga
     3721 tcctgaccgg cgtggtggtg gcgattcccg ggctgttcat cggcagcctg gtgctggaat
     3781 cgttcttcgg catccccggc ctgggcagct acacgatcga cgcgatcaac gcgcaggact
     3841 tcgcggtggt gcgggcgatg gtgttcctcg gggccgtgct ctacatcgcg ggcctgatcc
     3901 tcaccgacat ctcctacacg ctggccgatc cgcgagtgag gctgcaatga tgccttaccg
     3961 gtacatcatg ctgtggaccg atgcgctggt gtggctgctg gtgacgtgcg cgatcgggtt
     4021 cgcgtggtac gtggcgcagc acgagcattt gcgcgcaccg tggcgcaagg tggcgcagag
     4081 ccgctacggg atgtcggccc tggtggtgct gtgcgtgttc gtggcgatcg ggctgctcga
     4141 ttcgatccac taccaggaac ggctcgagac gcggcaggcg gacggcgccg ccaactactc
     4201 ggcggaggtg ctgagcctgt tcgacgccct ggccggccgc ctgcgcgaaa agcccgagcg
     4261 cacctactcc gcgcccctga cggcgtacgg cttcgccaaa gagaacgtga tgcgcccgga
     4321 cggcacgcag gcgcgcgagt ttccgcgcct gcagcacggc ggcgcgcacc tcaccgaccc
     4381 cgcgcggcag ctgccgctcg acgtgttgcg caagacgctg gccggcgccg ggctggcggt
     4441 ggcactgtgg atcgggctca ccatgctgct ggccgcctgg ctggcggcgc gccagagaca
     4501 acccttcggc gccaccctca ccgagatcat ccacggccgc acgcagattc cctggcggga
     4561 catgctgctc accgccgggg tggtgctgct cgtggcctgc ccgatgggcg tgctgagcgc
     4621 ctattaccac gtgctgggca ccgacaaggt cggccacgac gtcctgtacc tgactttgaa
     4681 gagcatccgc accgggctgg tgatcggcac gctcaccacg ctggtgctgc tgcctttcgc
     4741 gctcgtgctc gggctgatgg ccggttattt ccgcggctgg atcgacgaca tcatccagta
     4801 tctctacacc accttcagct ccattccggg cgtgctgttg atcgccgcgg cagtgctggc
     4861 cggccaggcg ctcatggacc gttatgcaga catgttcgag acggtcaccc agcgcgccga
     4921 cgccaggctg ttgttcctgt gcctgatcct cggcatcacc agctggaccg ggctgtgccg
     4981 cctgctgcgc ggcgaggcgc tgaagctgcg cgagatggac tacgtgcagg ccgcgcgcgc
     5041 gttcggcgtg ccggactggc gcatcatcaa ccgccatctg ctgcccaacg tgatgcacat
     5101 cgtgctgatc gccatcgtca tggacttcag cggcctggtg ctggccgagg cggtgctgtc
     5161 ctacgtcggc gtgggcgtgg atccgtccat gatcagcttc ggcaccatga tcaacaacgc
     5221 gcgcttggag ctggcgcgcg agccgatggt gtggtggtcg ctggccgccg ccttcgtgtt
     5281 catgttcctg ctggtgctct ccgccaacct gttctcggac gcggtgcgcg acgccttcga
     5341 tccgcgcatc cgcaccggct ggtcgttgtt cctgccgtac cgctcgcgcc cggcactggc
     5401 ggtggcgatc cgccgatgag cgcaccgccg ctgctcgagg tcgccgacct gcgcacctgg
     5461 ctgcacacgg ccaacggcgt ggtgcgcgcg gtggacggca tctcgttcac cgtgcagcgc
     5521 ggcgagacct tcgcgctggt gggtgaatcg ggttgcggca agtcgatgac cgcgttgtcg
     5581 ctgatgcggc tgctacccga tgccgggcgc atcgtcggcg gccgcgcact gctcgagggg
     5641 accgacctgc tggccctgcc ggaagcggcg atgcgcgagg tgcgcggcgc gcgcctggga
     5701 atcatcttcc aggagccggg caccagcttg aacccggtgc ttaccgtggg cgcccagatc
     5761 gaggaggtgc tgcagcagca cgcccgattg cgcgggccgg cggcgcgccg gcgcgcattg
     5821 gagctgctgg agcaggtcgg catcccggac gctgcgcgcc ggctcgacga gtatccgttc
     5881 cagatgtccg ggggcatgaa acagcgcgcc atgatcgcca tggcgctcgc ctgcaatccg
     5941 ggtctgctca tcgccgacga gccgaccacg gcgctcgacg tgaccatcca ggcccaggtg
     6001 ctggaggtgc tgcgcaggct gcagcgcgag gccggcatgt cgctgctgct catcacccac
     6061 gacctgggcg tggtcgcgga gatggcgcac cgggtggcgg tgatgtacgc cggggagatc
     6121 gtcgagtgcg caccgcgcga gcgcttcttc cgcgcgccgg cccatcccta ttcgcgcaag
     6181 ctgttcgagg cgctgcccgg cgccgcggga cgccatcgcc cgctggcggt gatccccggc
     6241 agcgtgccgc gcatggacga gagcttcgtc ggctgccgct tcgccgatcg ctgcgaccgg
     6301 gtgatggagg tctgccggcg ggaaccgccg cccgcggtcg atctcggcga tgggcaggtc
     6361 gtgctgtgcc atctgcatgc ggaggcgagg cacgaggcac ggggcgaaag ccgccaggca
     6421 cgaggcgtaa accaggtacg aggcacgagg gacgaggcag gcggggagga gacctcgcgc
     6481 ctcgtgcctc gtgcctcgag cctcatcgac gcgcctcgta cctcgagcct cctgcaagtc
     6541 gaagacctga aggtccactt cccgatccgc aagggcgtgt tcaagcgcgt cgtggggcac
     6601 gtacgcgcgg tggacggcgt cgcgctcgag atcccctcgc acaagacgct ggcgctggtg
     6661 ggcgagtccg gctgcggcaa gaccaccgtc ggcaaggggc tgctgcacct gctgccgctg
     6721 accggcggcc gcgtgctgtt cgagggcgag gatctggcga gcctgtcggc ccgacggctg
     6781 cgcgcccgcc gcaaggacat ccagatcatc ttccaggacc cgtacgcctc gctcaacccg
     6841 cgcatgcggg tgatggagat cgtcgaggag ggcatggccg cgctcggcat cggcgcatcc
     6901 gaggccgagc gccagcggcg ggtggacagg ctgctcgagc aggtgggact ggcgccggac
     6961 atgaagctgc gctacccgca cgagttctcc ggcggccagc ggcagcgcat cgccatcgcc
     7021 cgcgcgctgg cggtcgagcc caagctgatc gtctgcgacg agccgaccag cgccctggac
     7081 gtgtcggtgc aggcgcagat cctcaacctg ctgaaggacc tgcaaacgcg cctggggctc
     7141 gcctacctgt tcatcaccca caacatctcg gtggtcgagt tcctcgccca cgaggtcgcg
     7201 gtgatgtacc tgggccggat cgtcgagaag gggacggtgg aggaggtggt gggcagcccg
     7261 cgccatccct acacgcaagc gctgctgtcg gcggtgccca acatcgatgc ggccaccaag
     7321 cgcgaggtga tccgcctgca gggcgagttg ccgtcgccgt cgaatccgcc tgccggctgc
     7381 cacttccatc cgcgctgccc gcgcgcgatg cccgattgcc gggagaccta tcctggggag
     7441 gtcgcgctca gcggcacgca cagcgtgcgc tgcctgttgt acaagtgaag agaggcgttg
     7501 gaccaccccg tcgcgcttcg cgcgccacgc cttatgcgcc ctcaaggcgc gtcttaaaaa
     7561 aatccccgcg gagcgggcgg ggcggtgtgg ggtaagaacc taacgcttcg agacgcgctg
     7621 cttcgcggga gcgccgtaat gccagatggt gaggcgctgg cctatgcgta tggtggtgcc
     7681 gcgcagcccg ttccacgcct tcagcttgga caccgaagtg cggtagcggc gcgcgatgga
     7741 gccgagcgtg tcgccgctct tcacgcggta gatggtgcgc tggtggtagt cgtccgccgc
     7801 ggcctggaag tccttgctgg cgtaggtttc gtcgaggttg gtctcggctt cgtcgtgctc
     7861 cagcggcacc agcagcatct ggccggtgcg cacgcgcctg cctgcggcga ggccgttgac
     7921 ctccttgagc cgctcgaggc tgatgtcgaa cttgtccgcg acggtgtcga gcgtctcgcc
     7981 tttcttgagc tggtaggtct gccacgtcac cagcggctgg tcgttactct ccagattggc
     8041 ctggaacaga tccgccttgt ccgccggcac cagcagctgg cggttggcga ccggcgtgat
     8101 gacggggcgg tggtgcccgg gattgaggta gcggaactcc tcctcgggaa cgccggcgag
     8161 cttggccgcc atctcgacgt cgatgtgcgg cggcgcggtg accacggcga aatacggctc
     8221 gttgggaaga ggctccagcg tgaccttgaa cagctcgggg ttggagacga tgttcttcac
     8281 cgcctgcagc ttgggcaggt agttgcgcgt ctcgcgcggc agcttcaggt gccggtagtc
     8341 ggtcgacttg cgcgcgcgcc ggttcttcgc gatggcccgc tgcacgctgc cctctccggc
     8401 gttgtaagcc gcgagcgcca gctcccagtc gccgaacatg gtgtgcagaa actccaggta
     8461 gtccatggcc gcgcgcgtgg cggcgagcac gtcgcgccgc ccgtcgtacc accaggtctg
     8521 ctgcaggccg tacttgcggc ccgtggaagg gatgaactgc cacatgccgg atgcgcgcgc
     8581 acgcgagtag gccgtcgggt tgtaggcgct ctcgatcatc ggcagcagcg cgatctccat
     8641 cggcatgccg cgtttctcga cttcggcgac gatgtggtag aggtagcggc ggctgcggtc
     8701 gatcatgcgg gccacatact cgggccggtt caggtaccag gcctcgtgcc gcagcaccag
     8761 ggggctgtcg acctcttcca tcgcgaagcc cgcgcggatg cgctcccaca ggtcctcggc
     8821 cgagaccggc tcgacgacct ggccgttcca ctgcgccgcg ggcgtgccgt tgacatgggg
     8881 ggcctcgacc gcggggacga cctccaccgc gtcggaggcc accgggggca tagccaccgt
     8941 ggcgccgggg gcggcgctgg cggcgcggga cgaagcgatg ggggccgcga acagcgcggc
     9001 ttcggcgaag accgcgccgg ggaggcagag cgaggccgcc ccagcggcgg cagcagcggc
     9061 gaagaacagg gtgtttttca gcgtcatcgg gtctcgtatt gctttccgtt ctgaccgaat
     9121 cctagccaaa tattatcagt cacgtcaagg acttattttg ttaacttaac actgcttcac
     9181 gatcgaaggc aggccgctac gacgatgcaa gaagcaggcc ccgcagggga tggcgtagaa
     9241 tcgctgccat gtcgaatttg atggaccatc agctcgcccc cgactggttc gagacgccgc
     9301 tcggcaccca cctggtggcg tgcgagcagg cctacttcga tcacgaaacg gccgacgtgt
     9361 tcggcttcaa cgccatccag atcgggctgc cgcgcttcga tttcctgcgc gccaaccgca
     9421 tgccgctgcg ctgccgcgtc gcgcccgatg gcggggtgcg cgtgcgcgcc gacttcgagc
     9481 agctgccggt ctcgagcagc tgcgccgacc tggtgctgct gccccacgtg ttggagttca
     9541 gcgacaaccc gcaccaagtg ctgcgcgagg tgtcgcgcat tctgatgccc gaggggcacg
     9601 tgattgtatc cgggttcaat ccgtggagct tgtggggcgc ccgcgggttc ctcggcacgg
     9661 atcgcggcga ctttccgtgg cgcggccagt tcatcgcgct gccgcggctg cgggattgga
     9721 tggcactgct gggcatcgag atcgcggccg ggcgcatggg cgcctacgtg ccgccggtgc
     9781 gcagcgagaa gtggctgcgc cgcgtgagct tcctggaggc cgccggcgaa cgctg
//
LOCUS       DASPGE010000132         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_507459, whole genome shotgun sequence.
ACCESSION   DASPGE010000132 DASPGE010000000
VERSION     DASPGE010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507459"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VD791_04180"
     CDS             complement(<1..329)
                     /locus_tag="VD791_04180"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXV07201.1"
                     /translation="MMTDNALTLMNPAGLYDPAPNGYSHVALVAAGARLVYIAGQRGE
                     TESGALVPDFRLQVRQALRNIGACLASAGAGPAQVLRTKIYVTDIDAYVQEGHAEWLA
                     FFGEHLPA"
     gene            complement(387..1300)
                     /locus_tag="VD791_04185"
                     /pseudo
     CDS             complement(387..1300)
                     /locus_tag="VD791_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832385.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            1473..2576
                     /locus_tag="VD791_04190"
     CDS             1473..2576
                     /locus_tag="VD791_04190"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HXV07202.1"
                     /translation="MSIAATEPEATPLRPPPARLVDEGDSAPFVHAQDHDGQLTASNA
                     DLLSGKPVLLLFCPSVEDESLAAGLQAFRAAAPRLAALQATLLVICRSGIEANAAARA
                     RLALPFALLADANAAIFKGYGVDPHPAVPGLTLFILDAGHRVARVLDRMESAALIGEA
                     IASLERLAASRPMVALQAHPPVLVLPRMLGRADCGFLAEVWSRKVPEHGTDGFTNAGT
                     RSERGDFKVVHDGAYGMAVEYIVQDEALQRFIDGRLRRRLLPEIKKAFQATGLKREGY
                     RIAGYEARAGGFLNPHRDNSTPANAHRRFTMTINLNAGDYEGGALRFREYGEQLYEVE
                     RGTAIVWSASLLHEVLPVTRGRRLVLGVHMFGP"
BASE COUNT          444 a          896 c          933 g          401 t
ORIGIN      
        1 gccggcaggt gctcgccgaa gaacgccagc cactcggcgt ggccctcctg cacgtaggcg
       61 tcgatgtcgg tgacgtagat cttggtgcgc aacacctgcg ccggtccggc gccggccgac
      121 gcgaggcagg cgccgatatt gcgcagcgcc tggcgcacct gcagccggaa atcgggcacg
      181 agcgcgccgg actcggtctc gccgcgctgc cccgcgatat agacgaggcg tgcgcccgcc
      241 gccaccagcg ccacgtgcga atagccgttg ggcgcgggat cgtacaggcc cgccgggttc
      301 atgagagtca gcgcgttgtc agtcatcatg tcgggtttct cctcatcacc gagcatgacg
      361 cgcggacacg ccgtcgtgtg acatgctcag gctggcgcgg ttcgcctgcg tggtggcacg
      421 gttatggccg cgacgccggc aacgacgcag gcgagtccga tccaggcggt cgcgctcagt
      481 gtttcgccca ggaacagcac gccgatcgcg acgccgatcg gcacgcggag ataggcctgc
      541 gacgtggtgc ctaccgaacc caaggtatgg agcagccgga agtagatcgt gaaggcgagc
      601 gccgtcgaga acaccgagag cgcgatgagc gcgagaaccg attccagaga cggcgccaag
      661 gcccacggcc ggtcgatcgc aaggctggcg ggcacgagga tcgccgcgcc gcacagcatc
      721 gagccggcgg cgggcaggag cggatcaagc cccttgaagc cgcggccgaa gatcgccgcg
      781 cctgcatagc agatcgtggc gaagacgatc gccagctggg cggtgagctc gcgccccagc
      841 ccgcccagcg cctccacccc cacgatgagg ccgatgcccg tcaggcccgc gagcaggccc
      901 agcgccttgc gcaggctcac ggcttcgcgc cgcaccagca ggatcgccag caggaaggtg
      961 aagatcgggg aggtggcatt gaggatggtc gccaggcccg cgtcgaccgt ctgctccgcc
     1021 cacgcgatca gggtgaaggg aacgacgctg ttgaggcagg cctggaccgt gaagcgcagc
     1081 cagagcttcc gatcgcaggg gagatgcagc ccccgcgccc gcatgaccac cagcagcagc
     1141 ccgccggcga tcagcgtgcg cgccgcgatc agggtcaccg gcggaatggt ttccacgccc
     1201 accttgatga agctgtagga ggcgcccaca aggcggccaa tagtcccagc agcgcgagct
     1261 ccagcgcgag attcgcgttt cgaggcaagg tgttcaccat ggatggacgg cacggatcgc
     1321 gggtgacggg cgggccatcc tgtcacacac cgccgccgag gtcaggccgc aacggttcgg
     1381 ccgaaaccga aggaatgggt tggaaagcgc ggatcgcgcg tctatcgtga attgggattc
     1441 cgaacggggg aaggaattga gggcgcgttt ccatgagcat cgcggcgact gagccggagg
     1501 ccactccact gcgtccgccg ccggcgcgcc tggtggacga gggcgattcc gcgccattcg
     1561 tgcatgcgca ggatcacgat ggacagctta ccgcctcaaa cgccgacctg ctgagcggca
     1621 agccggtcct gctgctgttc tgcccgtcgg tggaggatga aagcctggcg gcgggtttgc
     1681 aggcgttccg tgccgccgcc ccacgcctcg ccgcgctgca agccaccttg ctcgtgatct
     1741 gccgcagcgg catcgaggcc aatgcggcgg cccgcgcgcg tctcgccctg ccctttgccc
     1801 tgcttgccga cgcgaacgcg gccatcttca agggctatgg cgtcgacccg catcccgccg
     1861 tgccggggct caccttgttc atccttgatg ccggtcaccg cgtggcgcgg gtgctggacc
     1921 ggatggaatc cgccgcgctg atcggcgagg ccatcgcgag tctcgagcgg ctggcggcga
     1981 gccgtccgat ggtggccctg caggcgcatc cgccggtgct ggtgctgccg cgcatgctgg
     2041 gccgggccga ttgcggtttc ctggccgagg tctggagccg caaggttccg gaacatggca
     2101 ccgacggctt caccaatgcc ggcaccagat ccgagcgcgg cgacttcaag gtcgtgcatg
     2161 acggcgccta cggcatggcc gtggagtata tcgtgcagga cgaagcgctg cagcgcttca
     2221 tcgacggtcg cctgcgccgg cgcctgctgc cggagatcaa gaaggcgttc caggcgaccg
     2281 gcctgaagcg cgaaggctac cgcatcgccg ggtacgaagc gcgggcgggc ggctttctca
     2341 acccccaccg ggacaactca acgccggcca atgcgcaccg gcgcttcacc atgaccatca
     2401 acttgaacgc cggggactat gaaggcggcg cgctgcgctt ccgcgagtat ggcgagcagc
     2461 tctatgaggt ggaacgcggc accgcgatcg tctggtcggc cagcctgctg cacgaggtcc
     2521 tgccggtcac cagaggccgg cgcctggtgc tgggcgtgca catgttcgga ccgtgacccc
     2581 aggcttgatc agtccttggc gctggaattc tcgttgcgtc tgtcgtcctt ggtgacgccg
     2641 accttcgagc ccttggaccc gccgcttctg gtgt
//
LOCUS       DASPGE010000133         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_390496, whole genome shotgun sequence.
ACCESSION   DASPGE010000133 DASPGE010000000
VERSION     DASPGE010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390496"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="VD791_04195"
     CDS             complement(<1..1121)
                     /locus_tag="VD791_04195"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXV07203.1"
                     /translation="MPNQPPKPQRRRRILRWILVVIAAVFLLPPLALVLIAWLEISIS
                     AGPWRGTIARTASAALGREVTLEGPLEFVPSLSPRLKVGGIRIANPPGFSTPEFASLG
                     DARLWVDTKALLQRRLRVREITAENVRVFLEIAEDRRVNWALGGARPAQPKGADGGGK
                     AALEAAKDFRFEVDTLGLRQLAVEFHNLPSGSKHFFNLDELAGEARRGQPMRLALRGS
                     VEKRFPYTLSFDGGTADELMRPTQPWPFQLGVEFLGTSFQANGAIARAAGGDSVEVLF
                     GLGTEDLREIERLLQTTLPNVGATALSGRVTADRLHVSLTELRGVMGRTTLEGELHYD
                     CSGKRPRVSGTLRLPTLDLRPFLTGEREGPEEPPGSLLDT"
     gene            1231..1782
                     /locus_tag="VD791_04200"
     CDS             1231..1782
                     /locus_tag="VD791_04200"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HXV07204.1"
                     /translation="MTARLFQLALACGLALPAAAAADIYRCVSPEGSTTYSDSPCPGG
                     AAKSANVTELIGACTTAECQARREQARAAAAARLQEEKAMVRQMQEHRLQAEALDLDR
                     RVRLETLRQLSVLDAAQRDVSSDAYYPVYPVLPGYGYGFGRRFDRPGCKGMQCGPVPG
                     PRPSGRHSRSHRGEPSVSVINPR"
     gene            1782..2225
                     /locus_tag="VD791_04205"
     CDS             1782..2225
                     /locus_tag="VD791_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXV07205.1"
                     /translation="MLSTSLPPKTLSAGIVAVCRDDARYRLLCLRAFDTWDFPKAQVL
                     GESDPLATAVAELQDATGIHDLEFPWGEDYRETVPYADGRVSRYYLAQVRSAEVALRQ
                     PAGADSAEDHEYRWVTFEEAEDILPPRLALVLDWVIGRLAGGRSR"
BASE COUNT          339 a          815 c          815 g          336 t
ORIGIN      
        1 gtgtcgagca ggctgcccgg cggctcttcc ggcccttcgc gctccccggt gaggaacggg
       61 cgcaggtcca gcgtcggtag ccgcagcgtg ccgctcacgc gcggccgctt gccgctgcag
      121 tcgtagtgca gctcgccttc cagcgtggtg cggcccatca cgccgcgcag ttcggtgagc
      181 gacacgtgca gccgatccgc cgtcacgcgc ccgctcagcg ccgtcgcgcc tacgttaggc
      241 agcgtggtct gcagcaggcg ctcgatctcg cgcagatcct cggtgcccag cccgaacagc
      301 acctccacac tgtccccgcc ggcagcgcgg gcgatggcgc cgttggcctg gaacgaagtg
      361 cccaggaact cgacaccgag ctggaacggc caaggctggg tgggccgcat caactcgtcc
      421 gccgtgccac cgtcgaagga gagcgtgtag ggaaagcgct tttcgacgct gccgcgcaac
      481 gcgagccgca tcggctggcc gcgcctggcc tcgcccgcca gctcgtcgag attgaagaaa
      541 tgcttgctgc cggagggcag gttgtgaaac tccaccgcga gctgccgcag tcccagcgtg
      601 tcgacctcga agcggaagtc cttggccgct tccagcgcgg ccttgccgcc gccgtccgcg
      661 cctttgggct gcgccggacg cgcccctccc agcgcccagt tcacccgccg gtcttcggcg
      721 atctcgagga agacgcgcac gttctcggcg gtgatctcgc gcacccgcag ccggcgctgc
      781 aacaacgcct tggtgtccac ccacagccgc gcatcgccca ggctcgcgaa ctcgggcgtg
      841 gagaacccgg ggggattggc gatgcggatg ccgcccactt tcaggcgcgg gctgagcgag
      901 gggacgaact cgagcggccc ctcgagcgtc acctctcgcc cgagggctgc gctggcggtg
      961 cgcgcgatgg tgccgcgcca gggcccggcg ctgatggaaa tctccagcca ggcgatgagc
     1021 accaaggcca gcggcggcag caagaacacg gcggcgatga cgaccaggat ccagcgcagg
     1081 atccggcgcc gtcgctgagg cttgggcggt tggttcggca cgcgggcggg cggccgggac
     1141 aagaccgcgc cgcaccgcct gtcattgtaa gtgtccggcc cggccaatgc cgcctataat
     1201 cgcaggagtg gttgccgcgg aggatcggtc atgactgccc gcttgttcca actggcgctc
     1261 gcctgcggcc tcgcgctgcc ggccgcggcc gcggccgaca tctaccggtg cgtgagcccc
     1321 gagggcagca ccacgtacag cgattccccg tgcccgggcg gcgccgccaa gtcggcgaac
     1381 gtgaccgagc tgatcggcgc gtgcaccacc gccgaatgcc aggcgcggcg cgagcaggcg
     1441 cgcgccgccg ccgcggcccg cttgcaggag gagaaagcca tggtgcgaca gatgcaggag
     1501 caccggctgc aggccgaggc gctcgatctc gatcgccgcg tgcggctgga gacgctgcgg
     1561 cagcttagcg tgctcgacgc ggcgcagcgc gacgtgagct cggatgcata ttacccggtc
     1621 tatccggtgt tgcccggtta cggctacggt ttcgggcggc gcttcgatcg gcccggctgc
     1681 aaggggatgc agtgcggccc cgtccccggc ccgcggccgt ccgggcggca ttcgaggtcg
     1741 catcgcggcg agccttcggt cagcgtgata aacccgaggt agtgctcagc acctccctgc
     1801 ctcccaagac cctgtcggcc ggcatcgtgg cggtatgccg cgacgatgcg cgctaccgcc
     1861 tgctgtgtct gcgcgccttc gacacctggg atttccccaa ggcccaagtg ctgggcgaga
     1921 gcgatccatt ggccactgcg gtggccgagc tgcaggatgc cacgggcatc cacgacctgg
     1981 agttcccctg gggagaggac taccgcgaga ccgttcccta cgccgacggc cgggtatcgc
     2041 gctactacct ggcgcaggtc cgcagcgccg aagtggcgct gcgccagcct gccggcgcgg
     2101 acagcgccga ggaccacgag taccgctggg tcaccttcga ggaggcggag gacatcctcc
     2161 cgccgcggct ggcgctggtg ctcgactggg tcatcggccg gctggccggc gggcgtagcc
     2221 ggtgacctga actatcgacg ccccgtgtag cctggatgga gccgcccaag gaccttgtcg
     2281 ataagacggg cggcgtaatc cgggg
//
LOCUS       DASPGE010000134         2679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_39182, whole genome shotgun sequence.
ACCESSION   DASPGE010000134 DASPGE010000000
VERSION     DASPGE010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39182"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VD791_04210"
     CDS             <1..566
                     /locus_tag="VD791_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXV07206.1"
                     /translation="EYVHDIHASGKHLLSLINDILDLSKIEAGKVELELSTFHLPSSL
                     EGTLTLVRERATRHGIEVGLKVDDKLGEFVADERKVRQVLLNLLSNAVKFTPDGGRVD
                     VRAEAVDSAVQIAVRDTGIGIAPEDQELIFEEFRQVGNDLFRKREGTGLGLTLARKFV
                     ELHGGRIWVQSELGKGSTFTFTLPLRS"
     gene            577..2463
                     /locus_tag="VD791_04215"
     CDS             577..2463
                     /locus_tag="VD791_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV07207.1"
                     /translation="MSAPATILVVDDTPHNVKLLADLLSVQGYAVVTAANGAEALAQV
                     RGIKPDLVLLDVVMPGMSGYEVCRHIRADASTGVLPVVMVTALDPTEERIKGLEAGAD
                     EFLTKPIHPPELLARVRSLLRIKSLYDTVQAQATELAGLNQGLEQRVQEQIGQLQRLA
                     QLKRFFPPQLAERIVGGDLDDPLATHRREVTVAFLDLRGFNAVADICEPEETMALLRS
                     YHREMGASIESHGGTLEQLSGNAMMVVFNDPVVVEDPAMRAVAMAMDMQQRFAQLMGD
                     WRKRGYEIALSIGIAHGYATIGAIGYEARVGYGVIGRVTNLAARLCAQAQPGQVLVTA
                     AVLALVEDKVDAQEVPQVDFQGFVHPVAAYGILGLKRPARAAEEPAWPLKIHALGQFA
                     LQVQGQPLTFSRKAQKKPLDLLRMLIALGGVRVETSALTGLLWPDAEGDAAKISFDSN
                     LYRLRKLLGVEDLLTLSEGKLSLDRERCWMDVWAVEDLVARVEREVRAGEGGAATACS
                     TLAKELLRLYGGHFLEKESQEPWAIAARDRLKAKFLRAVTVLGSGLEAAGQWEQAIAL
                     YSQALELDNLAEGLYRRLMVAYRERGEPAEALNVYRRCRHMLSVVLGAKPSAETEAIR
                     GTLG"
BASE COUNT          428 a          886 c          955 g          410 t
ORIGIN      
        1 tggagtacgt gcacgacatc cacgcctccg gcaagcacct gctctcgctg atcaacgaca
       61 ttctcgacct gtccaagatc gaggccggca aggtggagtt ggagctgagc accttccacc
      121 tgcccagttc gctggaaggc acgctcacgc tggtgcgcga acgggctacg cgccacggca
      181 tcgaagtggg cctgaaggtg gacgacaagc taggcgagtt cgtggcggac gagcgcaagg
      241 tgcgccaagt cctgctcaat ctgctatcca acgcggtcaa attcaccccg gacggcgggc
      301 gcgtggacgt gcgcgccgag gcggtcgatt ccgcggtgca gatcgccgtg cgcgacaccg
      361 gcatcggcat cgcacccgag gaccaggagc tgatcttcga ggagttccgc caggtcggca
      421 acgatctgtt ccgcaagcgc gagggcaccg gcctgggcct gacgctggcg cgcaagttcg
      481 tcgagctgca cggcgggcgt atctgggtgc agagcgagct gggcaagggc tcgaccttca
      541 ccttcacgtt gccgttgcgc tcgtgaggag gccgcgttga gcgctcccgc cacgatcctg
      601 gtggtggacg acacgccgca caacgtgaag ctgctggccg atctgctgtc ggtgcagggc
      661 tatgccgtgg tcaccgcggc gaacggggcg gaagccctgg cgcaggtgcg cggcatcaag
      721 cccgatctgg tgctgctcga cgtggtgatg ccgggcatga gcggctacga ggtgtgccgg
      781 cacatccggg cggacgcgag cacgggcgtg ctgccggtgg tgatggtcac tgccctggat
      841 cccaccgagg agcgcatcaa gggcctcgaa gccggggccg acgagttcct caccaagccg
      901 atccacccgc ccgagctgct ggcgcgcgtg cgctcgctgc tgcggatcaa gagcctgtac
      961 gacacggtgc aggcgcaggc caccgagctg gccgggctga accaggggct ggagcagcgc
     1021 gtgcaggagc agatcgggca gttgcagcgc ctcgcccagc tcaagcgctt cttcccgccg
     1081 cagctcgccg agcgcatcgt cggcggcgac ctggacgatc cgctggccac gcaccggcgc
     1141 gaggtcaccg tggccttcct cgacctcagg ggcttcaacg ccgtcgccga tatctgcgag
     1201 ccggaggaga cgatggcgct gctgcgcagc tatcaccgcg agatgggcgc ctcgatcgaa
     1261 agccacggcg gcacgctcga gcagctgagc ggtaacgcca tgatggtggt gttcaacgat
     1321 ccggtggtcg tggaagaccc cgccatgcga gcggtggcga tggccatgga catgcagcag
     1381 cgcttcgcgc agctcatggg cgactggcgc aagcgcggct acgagatcgc gctcagcatc
     1441 ggcatcgcgc acggctacgc gaccatcggc gccatcggct acgaagcgcg cgtgggatac
     1501 ggtgtgatcg gccgcgtcac gaacctggcc gcgcgcctgt gcgcgcaggc gcagccgggg
     1561 caggtgctgg tcaccgcggc cgtgctcgcg ctggtggagg acaaggtcga tgcacaggag
     1621 gtgccgcagg tggacttcca aggcttcgtg cacccggtgg ccgcctacgg aatcctgggg
     1681 ctgaagcgcc cggcgcgcgc ggccgaggag cccgcctggc cgctcaagat ccacgcgctc
     1741 ggccagttcg ccctgcaggt gcagggccag ccgctcactt tcagccgcaa ggcgcagaag
     1801 aaaccgctcg atctgctgcg gatgctgatc gcgctgggcg gcgtgcgcgt cgagaccagc
     1861 gcgctgaccg gcctgctgtg gcccgatgcg gaaggcgatg ccgccaagat ctccttcgac
     1921 agcaatctgt accgcctgcg caagctcctc ggcgtcgagg atctgctcac cttgtcggaa
     1981 ggcaagctct cgctcgaccg ggaacgctgc tggatggacg tgtgggcggt cgaggacctc
     2041 gttgcgcggg tggagcgcga ggtgcgcgcc ggcgagggcg gcgccgcaac ggcctgcagc
     2101 acgctggcca aggagctgct gcgcctctac ggcgggcatt ttctggagaa ggaatcgcag
     2161 gaaccctggg cgatcgccgc gcgcgaccgg ctcaaggcga agttcctgcg tgccgtgacg
     2221 gtgctcggct ccggcctgga ggcggcgggg cagtgggagc aggccatcgc gctctactcc
     2281 caggcgctgg agctggacaa cctggcggaa ggcctgtacc ggcgcctgat ggtcgcctac
     2341 cgcgagcgcg gcgagccggc cgaggcgctc aacgtctatc gccgctgccg gcacatgctg
     2401 tcggtggtgc tcggcgccaa gccctcggcc gagaccgagg cgatccgcgg cacgctgggt
     2461 tagcccgcat cgcaccgaca cctcgagttc tcccgtgtcg tcgatccgtc agtcattcgc
     2521 aagcgccccg ggcctatcct tgtctcgaag ctgtacgcga atttgccggt cggcgccgcg
     2581 ccgccggctg tcgcctatgg cccgatcggg taaagttcgc ttgagccgac cccatcgagg
     2641 tcccgagcgt tcggagcggc ctttgatcac cacacggac
//
LOCUS       DASPGE010000135         7739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_508301, whole genome shotgun sequence.
ACCESSION   DASPGE010000135 DASPGE010000000
VERSION     DASPGE010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7739
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508301"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VD791_04220"
     CDS             <1..1051
                     /locus_tag="VD791_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HXV07208.1"
                     /translation="LALVEEAFALLVARGVLAPVEATGSYSWGPERERLEALREMLRT
                     IPKHKGTDHLQADIKHRIKALSDELEAPRKGGARSAPALVVRPEGAAQIALLGPPNSG
                     KSALHARLTASHAHVADYPFTTQFPEPGMLPFEDIHFQLVDLPAVSPQHPVPWLAGAL
                     QTADACLLVMDLSDPDCVGEMQALHDILREHRVTLTPRWEPGRNASRAGVNADDPFAV
                     ELPALLLANKADRLDRVEAELAVFVELTGVAYPALAVSATTGRGLGDLGPWLFRNLGV
                     VRVYTKTPGHPPDKDRPFTLRRGQTVEDVARLVHRDLAHTLRYARVWGKSGFDGQQIG
                     REHVMGDGDVVELHG"
     gene            complement(1161..1613)
                     /gene="dtd"
                     /locus_tag="VD791_04225"
     CDS             complement(1161..1613)
                     /gene="dtd"
                     /locus_tag="VD791_04225"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HXV07209.1"
                     /translation="MIGLLQRVSEARVTVAGEVVGAIGPGLMVLVCAERGDDERSADR
                     LLERLLDCRVFSDAEGKMNLSVRDVGGGLLLVSQFTLAADTRKGNRPSFTPAAPPDEG
                     RRLFEHMLAKARASHAPVEAGRFGADMQVALVNDGPVTIWLRVDPADS"
     gene            1702..2268
                     /gene="msrA"
                     /locus_tag="VD791_04230"
     CDS             1702..2268
                     /gene="msrA"
                     /locus_tag="VD791_04230"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HXV07210.1"
                     /translation="MTQLRSNTAPPGREIATLAGGCFWCLEAVYDQVKGVDSVESGYM
                     GGKLANPTYRDVCNGDTGHAEVVRVTFDPKVVSFRDLLDVFFVIHDPTTPNRQGNDVG
                     TQYRSAIFYHSPEQRQIAEDAIRRLADEGVYDDRIVTEVTPASGFYTAEDYHQEYFAN
                     NPYQPYCMAVVAPKVTKFRKQFLDRLKK"
     gene            complement(2293..3630)
                     /locus_tag="VD791_04235"
     CDS             complement(2293..3630)
                     /locus_tag="VD791_04235"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXV07211.1"
                     /translation="MTGHEILGLLMLGAMISIIFVGFPIAFTLLFIALLFGTLGLGAE
                     LTFSLAYLQVWGTMKDDILPAVPLFIFMGFMTEQAGLMERLFLALRNLLAPLRGSLFV
                     AVILTAAIFAMATGIVGAAVTVLGIMAGPVMMRARYDARLSAGAIAAGGTLGILIPPS
                     VMLVVMGPTIGVPVNLLYASALGPGLLLAAMYLAYCLMRSFLKPELGPPVPKAERVTS
                     RMELAWELAVGVVPLAALVGFTLGTILAGVATPSEAAACGASGATLLALAYRRLTFQT
                     FKNAALSTMVTSSMVLFLAVASNVFGAVFTKFGATELISGTLGQLPLPGMGKLLLVMA
                     LIFLLGWPFEWPAIILVFLPIFLPVVEQLDLGLERGEMLIWFGALVAVNLQTAFLSPP
                     VAMSAYYLKNVMPQWNLSLIYRGMGQFMIIQLLCLAAVLLWPQLALWLPRAMQ"
     gene            complement(3640..4191)
                     /locus_tag="VD791_04240"
     CDS             complement(3640..4191)
                     /locus_tag="VD791_04240"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HXV07212.1"
                     /translation="MSRPSPVYLAAIRSIDRVTRWSGYTFALLVIPLVLANTVEVFMR
                     YVLDRPTVWALETTVMSYGALFMLGAAYALLKGAHVRTDLLWEKFSDRTKGVIDSVAY
                     ALLFLPSMALLFYLSFDELVYAWSIDERSNYTVWQPPLWPLRAVVPVSALLLFLQGVS
                     ELLKSLWAARTGKMLVHHEHIEI"
     gene            complement(4244..5332)
                     /locus_tag="VD791_04245"
     CDS             complement(4244..5332)
                     /locus_tag="VD791_04245"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXV07213.1"
                     /translation="MSALRLFAAALALASAAAAAQPVTKLRFQGAFPASSLMFENSKF
                     FTERVKALSGGRLQIEMLPPGAVVPPFEVLDAVHKGVVDGGHTAPAYWLGKNRASTLF
                     GPAPGGPFGMDMLDYMGWLYEGGGLEFYREFYQKELKRNVVPVPMTSVANQILGWFKT
                     PVKSWEDLKGRKCRETGITAEVFSKAGMNPVNVPGGEIVPAGQRGVIECAEFVGPAED
                     MKIGFHTIWKYVYMPSTHENATVVELLINGDVWNKLRPEHREIIQSAAFEATLRSQLV
                     SNRLNAEALVELRSKHGVKIERTPDDILTKILIAWDQIAKEESAKNPFFRKVYESQRA
                     YAAKVVPARREVYAPYELGANYYWPAKK"
     gene            complement(5329..6381)
                     /locus_tag="VD791_04250"
     CDS             complement(5329..6381)
                     /locus_tag="VD791_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXV07214.1"
                     /translation="MKGVVFQGNRKLELMDFPDPTPGPGEVVLEIKASGMCGSDLKYY
                     RAAGGAASMGLGAVSGPIIGGHEPCGVVAAIGPGVSDRQAKVGQRVMQHHYRGCGVCE
                     HCATGWMQLCVEGVAEVYGATGHGAHARYMKCPARTLVPLPDELSFEAGAAVSCGTGT
                     AWGALHRLDLLGDHTIAVFGQGPVGLSATLLAAQMGARVIALDTSEERLARAKEFGAD
                     VLINPKTTANVVQAIRDLTHGRGAHASLDASSSPEARAQAVRCVRTWGKACFVGEGDS
                     VTLDVSNDLLRRQVTLIGSWTFSSVGQADCARYVADRKIVLDRLFTHRWRLEQAEEAY
                     RLFDRQTSGKGVILPS"
     gene            complement(6398..7732)
                     /locus_tag="VD791_04255"
     CDS             complement(6398..7732)
                     /locus_tag="VD791_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HXV07215.1"
                     /translation="MAAAVAAFPGWKRTSAPARAEMLVEVARRLRAGREDLAVTLTRE
                     TGRTLLKNRGYVDWAATCFEYYAGLIRDRRGRVIPSAADGQLNMVLKEPLGVVGCIVP
                     FNYPLMLLAWKVAPALAAGCTVVTKPSEFTSASTLEFAKLVEEAGFPPGVFNVVTGFG
                     RDVGTPLVSHPLVRKIAFTGSDATGRAINEAAAKSFKRVTLELGGKSPNIVFADAKIE
                     DAVNGAVSGIFAATGQTCIAGSRLLLQESVHDQFTDKLLGIAKTARMGNPMSYDTQVG
                     PITTRPQYDKVLSYIDIAKKEGAQLLMGGGPASRPECGDGWFVEPTVFAGVRNDMRIA
                     QEEVFGPVLSVIKFKDEDEAAAIANDVRFGLGAGVWTNDIGRAIRMAERIQAGTVWVN
                     TYRAVSYMSPFGGYKDSGLGRENGIDAIREYLQVKSVWINTGAATANPFVMR"
BASE COUNT         1275 a         2735 c         2569 g         1160 t
ORIGIN      
        1 tctggcgctc gtcgaggagg cgttcgcgct gctggtcgcg cgcggcgtcc tggcgcccgt
       61 cgaggcgact ggctcgtaca gctggggtcc cgagcgcgag cgcctcgagg cgctgcgcga
      121 gatgctgcgc accatcccca agcacaaagg caccgaccac ctccaggcgg acatcaagca
      181 ccgcatcaag gcgctgtccg acgagctgga ggccccgcgc aagggcggcg cgcgcagtgc
      241 gcccgcgctg gtggtgcgtc ccgagggcgc tgcgcagatc gcgctgctgg gtccgcccaa
      301 ctcgggcaag tccgccctgc acgcgcgcct cacggcctcg cacgcgcacg tggccgacta
      361 tcccttcacc acgcaattcc ccgagcccgg catgctgccg ttcgaggaca tccacttcca
      421 gctcgtggac ttgccggcgg tgtcgcccca gcatcctgtg ccctggctgg cgggggcact
      481 gcagaccgcc gacgcctgcc tgctggtgat ggacctgagc gacccggact gcgtgggaga
      541 gatgcaggcg ctgcacgaca tcctgcgcga gcaccgcgtg acgctaaccc cgcgctggga
      601 accggggcgc aatgcctccc gggccggcgt gaacgcggac gatccgttcg ccgtcgaact
      661 gcctgcgctg ctgctcgcga acaaagccga ccgcttggac cgggtggagg ccgagctggc
      721 ggtgttcgtg gaactgaccg gggtcgccta tcccgcgctg gccgtgtccg ccacgaccgg
      781 gcgcgggctg ggagacctcg gcccctggct gttccgcaat ctcggcgtgg tgcgcgtgta
      841 caccaagacc ccgggtcatc cgccggacaa ggatcggccc ttcacgctgc gccgcgggca
      901 gaccgtcgag gacgtggcgc ggctggtgca tcgggacctc gcgcacacgc tgcgctacgc
      961 gcgcgtgtgg ggcaagtccg gattcgacgg gcagcagatc ggccgcgagc acgtcatggg
     1021 cgacggcgac gtcgtcgagc tgcacggctg agccgcgcta ggcactcccc tcctgccaag
     1081 gaggggtgcc cgcgaagcgg gcggggtggt gcgaaggcgg ttgcgcggca aacaccattg
     1141 cgagcttgcg gcaagctcaa tcagctgtcc gccggatcga cccgcagcca gatcgtcacc
     1201 gggccgtcgt tcaccagcgc gacctgcatg tcggcgccga agcggccggc ctcgaccgga
     1261 gcatggctcg cgcgcgcctt ggccagcatg tgctcgaaca gccgccggcc ctcgtccggc
     1321 ggcgcggcgg gcgtgaagct cggccggttg cccttgcggg tgtcggccgc cagcgtgaat
     1381 tgcgacacca gcagcagccc gccgccgaca tcgcgcacgc tcaggttcat cttgccctcg
     1441 gcgtccgaga acacgcggca gtcgagcaag cgctcgagca ggcggtcggc gctgcgctca
     1501 tcgtccccgc gctcggcgca caccagcacc atcaggccgg gtccgatcgc accgaccacc
     1561 tcgccggcca ccgtcacccg cgcctcgctc acgcgctgca gcaggccgat catcggctac
     1621 acgcagccgc aacgacccgg cccgggatga tgtaaggtga catggaaagc tgcgacacac
     1681 tctggagcga ggagcgagat tatgacccag ctgcgatcga acaccgcgcc gcccgggagg
     1741 gagatcgcca cgctggccgg cggctgcttc tggtgcctgg aggcggtcta cgaccaggtg
     1801 aagggcgtgg acagcgtcga gtcgggctac atgggcggga agctcgccaa ccccacgtac
     1861 cgcgacgtgt gcaacggcga caccggtcac gccgaggtgg tgcgcgtgac cttcgatccc
     1921 aaggtcgtga gctttcgcga cctgctggac gtgttcttcg tgatccacga tcccacgacc
     1981 ccgaatcgcc agggcaacga cgtgggcacg cagtaccggt ccgcgatctt ttatcactct
     2041 cctgagcagc ggcagatcgc cgaggacgcg atccggcggc tggccgacga aggcgtctac
     2101 gacgaccgca tcgtcaccga agtgacgcct gcgagcggct tctacaccgc cgaggactac
     2161 catcaggagt atttcgccaa caacccctac cagccctact gcatggcggt ggtggcgccc
     2221 aaggtcacca agttccgcaa gcagttcctc gaccggctca agaaatagcc ctgcggcgcc
     2281 agccccggcg attcactgca tcgccctggg cagccacagc gccagctgcg gccacagcag
     2341 cacggcggcc aggcacagca gctggatgat catgaactgg cccatgccgc gatagatcag
     2401 ggacaggttc cactgcggca tcacgttctt caggtaatag gccgacatcg ccaccggcgg
     2461 gctgaggaag gcggtctgca ggttcaccgc caccagcgcg ccgaaccaga tcagcatctc
     2521 gcccctttcc aggcccaggt cgagttgctc gaccacgggc aggaagatcg gcaggaacac
     2581 caggatgatg gccggccact cgaacggcca gcccagcagg aagatcagcg ccatgacgag
     2641 cagcagtttg cccatgcccg gcagcggcag ctgcccgagc gtgccgctga tcaactcggt
     2701 ggcgccgaac ttggtgaaca cggcgccgaa cacgttcgac gccacggcca ggaacagcac
     2761 catgctcgag gtgaccatgg tggacaacgc cgcattcttg aaggtctgga aggtgagccg
     2821 ccggtaagcg agcgccagca gcgtcgcgcc ggaggcgccg caggcggccg cttcgctggg
     2881 cgtggccaca ccggcgagga tggtgccgag cgtgaagccg accagcgcag ccagcggcac
     2941 tacgccgacc gccagttccc aggccagttc catccggctg gtgacgcgtt ccgccttcgg
     3001 cacgggtggc ccgagctccg gtttcagaaa gctgcgcatc aggcaatagg ccaggtacat
     3061 ggcggccagc agcaggcccg gtccgagggc cgaggcgtag agcagattca cgggtacgcc
     3121 gatcgtcggt cccatcacca ccagcatcac gctgggcggg atcaggattc ccagggtgcc
     3181 gccggcggcg atcgccccgg cggacagccg cgcgtcgtag cgcgcgcgca tcatcaccgg
     3241 cccggccatg atgcccagca ccgtcaccgc ggcgccgacg atgccggtgg ccatggcgaa
     3301 gatggccgcg gtgagaatca ccgccacgaa caacgagccg cgcagcggcg ccagcaggtt
     3361 gcgcagcgcc aggaacagcc gctccatcag ccccgcctgc tcggtcatga agcccatgaa
     3421 gatgaacaag ggcaccgccg gcaggatgtc gtccttcatc gtgccccaca cttgaagata
     3481 ggcgaggctg aaggtgagct cggcccccag gcccagcgtg ccgaacagca gggcgatgaa
     3541 cagcagcgtg aaggcgatcg gaaacccgac gaagatgatg ctgatcatcg cccccagcat
     3601 cagcagcccg aggatctcgt ggccggtcat gatgcggggc tagatctcga tgtgctcgtg
     3661 gtgcaccagc atcttgcccg tgcgcgccgc ccacaggctc ttcagcagct cggacacgcc
     3721 ttgcaggaac agcagcagcg cgctgacggg caccaccgcg cgcaacggcc acagcggcgg
     3781 ctgccagacg gtgtaattgg aacgctcgtc gatcgaccag gcgtagacca gctcgtcgaa
     3841 ggacagatag aacagcagcg ccatcgaggg caggaacagc agcgcgtagg cgacgctgtc
     3901 gatgacgccc ttggtgcggt ccgagaactt ctcccacagc aggtcggtgc gcacgtgcgc
     3961 gcccttgagc agggcatagg ccgcgccgag catgaatagc gcgccgtagg acatcaccgt
     4021 ggtttccagc gcccacaccg tgggccggtc gagcacgtag cgcatgaaca cctcgacggt
     4081 gttcgccagc accagcggga tcaccagcag cgcgaaggtg tagccgctcc accgcgtcac
     4141 gcggtcgatg ctgcggatgg cggcgagata gacgggggac ggccggctca tggctgcgcc
     4201 gagccggcgc gcgggcgacg ccggcccgag tcgaaccggg ccgtcacttc ttcgcgggcc
     4261 agtagtagtt cgcgcccagc tcgtaggggg cgtacacctc gcgccgtgcc ggcaccactt
     4321 tcgcggcgta ggcgcgctgc gactcgtaga ccttcctgaa gaacgggttc ttggccgact
     4381 cctccttggc gatctggtcc caggcgatga ggatcttggt caggatgtcg tcgggcgtgc
     4441 gctcgatctt caccccgtgc ttgctccgca actcgaccag cgcctccgcg ttgaggcggt
     4501 tggagacgag ctgcgagcgc aaggtggcct cgaacgcagc cgactggatg atctcgcggt
     4561 gttccggccg cagcttgttc cagacgtctc cgttgatcag cagctcgacc acggtggcgt
     4621 tctcgtgggt cgagggcatg tacacgtact tccagatggt gtggaagccg atcttcatgt
     4681 cctcggccgg gccgacgaac tcggcgcact cgatcacgcc gcgctgcccg gccggcacga
     4741 tttcgccgcc cggcacgttc accggattca tcccggcctt gctgaacacc tcggccgtga
     4801 tcccggtctc ccggcacttg cggcccttga gatcctccca gcttttcacc ggcgtcttga
     4861 accagcccag gatctgattg gcgaccgagg tcatcggcac cggcacgacg ttgcgcttga
     4921 gctccttctg gtagaactcg cgatagaact ccaggccgcc cccttcgtac agccagccca
     4981 tgtagtcgag catgtccatg ccgaagggtc cgcccggggc cggcccgaac agcgtcgatg
     5041 cgcgattctt gcccagccag taggccggag ccgtgtgccc gccgtccacc actcccttgt
     5101 gaaccgcgtc gagcacctcg aagggcggca ccactgcccc cgggggcagc atctcgatct
     5161 gcaggcgacc gccgctgagc gccttgacgc gctccgtgaa gaacttcgag ttctcgaaca
     5221 tcaggctcga ggcggggaac gcgccctgga agcgcagctt ggtcaccggc tgcgccgccg
     5281 ccgctgccga cgccagcgcc agtgcggccg cgaaaagtcg cagagcgctc atgacggcag
     5341 gatcacgccc ttgcccgagg tctgccggtc gaacagccgg taggcttcct ccgcctgctc
     5401 gagccgccag cggtgggtga acaggcgatc gagcacgatc ttgcgatcgg cgacgtagcg
     5461 cgcgcagtcg gcctgcccga cgctggagaa ggtccacgag ccgatcagcg tcacctgccg
     5521 gcgcagcaga tcgttgctga cgtcgagtgt gacgctgtcg ccttcgccga cgaagcaggc
     5581 cttgccccag gtgcgcacgc aacgcaccgc ctgcgcgcgc gcctccggcg acgaggacgc
     5641 gtccagcgac gcgtgcgcgc cgcggccgtg cgtgaggtcg cggatcgcct gcaccacgtt
     5701 cgcggtggtc ttcgggttga tcagcacatc ggcgccgaac tccttcgccc gcgccagccg
     5761 ctcctcgctc gtgtccagcg cgatcacgcg ggcgcccatc tgcgctgcga gcagcgtggc
     5821 cgacagcccc accggcccct gaccgaacac cgcgatggtg tgatctccca gcagatccag
     5881 gcggtgcagc gcgccccacg cggtgccggt gccgcaggaa acggcggcgc cggcctcgaa
     5941 cgacagctcg tcgggcagcg ggaccagcgt gcgcgcgggg catttcatgt agcgcgcgtg
     6001 ggcgccgtga ccggtggcgc cgtacacctc ggccacgccc tccacgcaca gctgcatcca
     6061 gccggtggcg caatgctcgc acaccccgca gccgcgataa tgatgctgca tcacgcgctg
     6121 gccgaccttc gcctgtctgt cggagacgcc cggcccgatc gcggccacca caccgcacgg
     6181 ctcgtggccg ccgatgatcg ggccgctcac cgcgcccagg cccatcgagg cggcgccgcc
     6241 cgccgcgcgg tagtacttga ggtcgctgcc gcacatgccc gacgccttga tctccagcac
     6301 cacttctccc ggacccggcg tcgggtccgg gaaatccatg agttccagct tacggttgcc
     6361 ttgaaacacc acgcctttca tcgcttgctc ctcgcctcta gcgcatcacg aacgggttgg
     6421 ccgtcgccgc gccggtgttg atccacacgc ttttcacctg caggtactcg cggatcgcat
     6481 cgattccgtt ctcgcggccc aggccggagt ccttgtagcc gccgaagggg gacatgtagc
     6541 tcaccgcgcg gtaggtgttg acccatactg tcccggcctg gatgcgctcc gccatgcgga
     6601 tggcgcgccc gatgtcgttg gtccacacgc ccgcgcccag gccgaagcgc acgtcgttgg
     6661 caatggccgc ggcctcgtcc tcgtccttga atttgatcac cgacagcacc gggccgaata
     6721 cctcctcctg cgcgatgcgc atgtcgttgc gcacccccgc gaacacggtg ggctcgacga
     6781 accagccgtc gccgcattcc gggcgcgacg cggggccgcc gcccatcagc agctgcgcgc
     6841 cctccttctt cgcgatgtcg atgtaactca gcaccttgtc gtattgcggg cgtgtggtga
     6901 tcggcccgac ttgggtgtcg tagctcatgg gattgcccat gcgcgcggtc ttggcgatgc
     6961 cgagcagctt gtccgtgaac tggtcgtgca cgctttcctg cagcagcagg cgcgagccgg
     7021 cgatgcaggt ctggccggtg gcggcgaaga tgccggacac cgcgccgttg accgcgtctt
     7081 cgatcttggc gtcggcgaac acgatgttgg gcgactttcc gcccagctcc agggtgacgc
     7141 gcttgaagct cttggcggcc gcctcgttga tcgcgcggcc ggtggcgtcc gagccggtga
     7201 aggcgatctt gcgcaccagc gggtggctga ccagcggggt gcccacgtcg cggccgaaac
     7261 cggtgacgac gttgaacacg cccggcggga agccggcctc ctcgaccagc ttcgcgaact
     7321 ccagcgtcga ggccgaggtg aactccgaag gcttggtcac caccgtgcag ccggcggcca
     7381 gcgccggcgc gaccttccac gccagcagca tcagtggata gttgaacggc acgatgcagc
     7441 cgaccacccc gagcggctcc ttgaggacca tgttgagctg gccatcggcg gcggatggga
     7501 tgacacgccc acgccgatcg cgaatgagcc cggcgtaata ctcgaagcag gtcgccgccc
     7561 aatcgacgta tccccggttc ttcagcagcg ttcgaccggt ctcccgggtg agggtcacgg
     7621 cgagatcctc gcgcccggcg cgcagcctgc gggcgacctc caccagcatc tccgcacgcg
     7681 ccggggcgct ggtgcgcttc cagccgggga acgcggcgac ggcggccgcc accgccgcc
//
LOCUS       DASPGE010000136         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_976174, whole genome shotgun sequence.
ACCESSION   DASPGE010000136 DASPGE010000000
VERSION     DASPGE010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976174"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..887)
                     /locus_tag="VD791_04260"
     CDS             complement(201..887)
                     /locus_tag="VD791_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXV07216.1"
                     /translation="MLTRKQAEAADARRQAEPAPGAPRLIRVLIADDHPLVRRGLAAI
                     INMEEDASVVGEAGDGEEAVVLWRRLRPDVTLMDLRMPKLEGVQAIRQIRAEDPGAGI
                     IVLTTFDHDEDIYAGLRAGAKAYLLKDVQPEELFRCIRTVHAGEAYLQPRVAAKLAQR
                     MHEETLTEREVQILRLLAEGKSNRAIGQALFITEGTVKSHLKSLFVKLDVTSRAEAIA
                     LAAKRGLVKF"
     gene            complement(893..2233)
                     /locus_tag="VD791_04265"
     CDS             complement(893..2233)
                     /locus_tag="VD791_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HXV07217.1"
                     /translation="MSPKPLAMERPSGRPAESGRGPLPPREVGHWPDARVRALEEANR
                     ILQQEVEQHRLAEEMARGQSQMLIESLGFLAAESNLDTFLGHVLKATVQQLEGVGGTL
                     WFPDPLTGTARLHLEYLDTRVIPADESHHPAVLQPLPIGGRGVSTFPMIHPETYVLTK
                     TVAGMPEENRAYIRSLGVRLLLTVPMVLGRETVGWICVRSGRSDASEMESKIHLAEAL
                     AGQATLAVQMARLGEQAREAAVLGERNRIARDIHDTLAQGFTGVVVNLEAASRTLKKQ
                     NLAVALEHIEHARGLAQSCLDEARLSVRALRPDPLRHADLVEALQAQVQRLEASGTHA
                     QLTVLGDRIPLLREVQAELLRIAQESITNVMKHARAHSVHVTLAFGEDAVSLSVGDDG
                     VGFDPNARHDGFGLLGMRERAERIGARLVVQSRARGGSRVETLLPRSALGGGGR"
BASE COUNT          341 a          882 c          808 g          400 t
ORIGIN      
        1 ctcttcgagg ggacaggaaa gagcggcgcg cgactgcgcc gcgctacgag caggcgaaga
       61 aattgtcggt gccggcccgc gggggtgcat ccggcggagg aaggaattcg ccgatgcctt
      121 tcgtgggagt cgcgcctccc cctttcgagg gaggctgcgg cgcgaaacgc cgcgtttctc
      181 acccttgcga gcgagcccgg tcagaacttc accaggccgc gcttggccgc gagcgcgatg
      241 gcctcggccc ggctggtgac gtcgagcttg acgaacaggc tcttcaggtg gctcttcacc
      301 gtgccctcgg tgatgaacaa cgcctggccg atggcgcggt tgctcttgcc ctcggcgagc
      361 agtctcagga tctgcacttc ccgctcggtc aacgtttcct cgtgcatgcg ctgcgccagc
      421 ttcgcggcca cccgcggctg cagatacgct tcgcccgcgt gcacggtgcg gatgcaccgg
      481 aacagctcct ctggctgcac gtccttgagc agataggcct ttgcgcccgc gcgcaagccg
      541 gcgtagatgt cctcgtcgtg gtcgaacgtg gtgagcacga tgatgccggc gcctgggtcc
      601 tcggcgcgga tctggcggat cgcctggacg ccctcgagct tgggcatgcg caggtccatc
      661 agcgtcacgt ccgggcgcag ccggcgccac agcacgactg cctcctcgcc gtccccggcc
      721 tcgccgacca cgctggcatc ctcctccatg tttatgatcg ccgccagccc acggcgcacc
      781 agcggatggt cgtcggcgat cagcacgcgg atcaaccgcg gcgcgccggg cgcgggctcg
      841 gcttggcgcc gcgcatcggc cgcttctgct tgcttgcgtg tgagcatggg ggctagcgcc
      901 cgccgccgcc cagcgcactg cgcggcagca gggtctccac gcgcgagccg ccgcgggcgc
      961 ggctttgcac caccaggcgg gcgccgatgc gctcggcgcg ctcgcgcatg ccgagtagcc
     1021 cgaaaccgtc gtgacgcgcg ttcggatcga aaccgacgcc gtcatcgcca accgacagcg
     1081 ataccgcatc ttcgccgaag gcgagcgtca cgtgcacgct gtgcgcccgg gcatgcttca
     1141 tcacattggt gatggactcc tgcgcgatgc gcagcagctc ggcctgcacc tcgcgcagca
     1201 ggggaatcct gtctccgagc acggtgagct gcgcgtgggt gccgctcgcc tccaggcgct
     1261 gcacctgcgc ctgcagcgcc tccaccagat cggcgtgccg cagcggatcg ggccgcagcg
     1321 cgcgcaccga caggcgcgcc tcgtccaggc acgattgcgc cagtccgcgg gcgtgctcga
     1381 tgtgctccag agcgaccgcg aggttctgct tcttcagcgt gcggctggcg gcttccagat
     1441 tgaccaccac gccggtaaat ccctgcgcca gcgtgtcgtg gatgtcgcgc gcgatgcggt
     1501 tgcgctcgcc caggacggcg gcctcgcgcg cctgctcgcc gagccgcgcc atctgcaccg
     1561 ccagcgtcgc ctggccggcc agcgcttcgg ccagatggat cttgctctcc atctcgctcg
     1621 cgtcgctgcg gccgctgcgc acgcagatcc agccgacggt ctcgcggccc agcaccatcg
     1681 gcaccgtgag cagcaggcgc acgcccagcg accggatgta ggcgcggttc tcctccggca
     1741 tgccggcgac cgtcttggtc aggacatagg tttccggatg gatcatggga aacgtcgaca
     1801 cgccgcggcc gccgatcggc aacggctgca gcacggcggg gtgatgggac tcgtccgccg
     1861 ggatcacgcg cgtgtcgagg tactccaggt gcagccgcgc ggtgccggtg agcggatccg
     1921 ggaaccagag cgtgccgccg accccttcga gttgctgcac cgtggccttg agcacgtgcc
     1981 ccaggaaggt gtccagattg gattcggcgg cgagaaaccc gagggattcg atcagcatct
     2041 gcgactggcc ccgagccatc tcctcggcca gccggtgctg ctcgacctcc tgctgcagaa
     2101 tgcggttggc ctcttccagc gcgcgcacgc gggcgtccgg ccagtgcccc acttcccggg
     2161 gcggcagcgg accgcgtccg gactctgccg gccggcccga cgggcgctcc atcgccaggg
     2221 gcttggggct catcgcatac tcctcctcgc gcgcattatc cggccgaggg cgaaccacgt
     2281 ctacagcgac ccgcaccccg ccaagcttcg cggccgtggc ttgatccccg gattccgctc
     2341 cgctgcatcc gggctacgtc tattttgttg ctgcgttaac cccgccagaa tcctcgtgcg
     2401 tttcaagcct caagaaccct tcacccgaca a
//
LOCUS       DASPGE010000137         5899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1093919, whole genome shotgun sequence.
ACCESSION   DASPGE010000137 DASPGE010000000
VERSION     DASPGE010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093919"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VD791_04270"
     CDS             complement(<1..1075)
                     /locus_tag="VD791_04270"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HXV07218.1"
                     /translation="MPSPPIAPREPFVTQVHGQPRVDPYHWLRDKDNPRVRAHLQAEN
                     AYTTAMMAHTEAFREALYREMLARIRETDMDVPYREGRFEYYGRTEQGQDYPILCRRP
                     LSGGAEQVTLDMNALAQGHEFFALGAYDVSGDADLLAFSTDITGFREYTLQVKDLRTG
                     ELLPLRVEHTRSVAWGNDHRTLFYVVEDEAKRACRLYRHRLGDERHELLYEETDERFS
                     IGVDQSRSRAYLFLTAHSATTSEVRYLRADDPGGAFALALPRRHEHEYYLDHHGERFY
                     IVTNDRGRNFRLVTAPVDDPRESGWLELIPHRDDVMLDGVDLFAHHYVVHERRGGFPK
                     LRVVRLATGDSHDIEFPEPVYAVH"
     gene            1143..1562
                     /locus_tag="VD791_04275"
     CDS             1143..1562
                     /locus_tag="VD791_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07219.1"
                     /translation="MWVRILGSVLALAAAAPTPGQDKIEAAQDKVEAGSVIWHDRRCA
                     FFIVQSSWGFTLFEYLNGPWPSLDDVIEGKLEGFGTRNVTNKTVDNQITMVYSEISST
                     SKKWVGGKIPGFCRRKKDFLAQLQGEGAPPAPAPAQQ"
     gene            1559..1753
                     /locus_tag="VD791_04280"
     CDS             1559..1753
                     /locus_tag="VD791_04280"
                     /inference="COORDINATES: protein motif:HMM:NF023369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HXV07220.1"
                     /translation="MKNVRYRSEFTQFIDAFLERNPQVVEKQKRHRATWWDRPQDLAE
                     RERQEQADVAPTGYAYYHNP"
     gene            1856..3058
                     /locus_tag="VD791_04285"
     CDS             1856..3058
                     /locus_tag="VD791_04285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV07221.1"
                     /translation="MSAKKNVAALAVCQGLLVTNNIAVISIGSLAGYMLAANKALSTL
                     PATAYIAGGALSTYPISLFMKRHGRRAGFTLGAAAGLLGALICTLAVAAGSFALFCLG
                     TFIAGTYTASGGYYRFAAADAVGMGFKSRAISLVLAGGILGGILGPESSKLTKDWLPV
                     TFMGSYAALMAFALIVIAILRLVHLPMPSEQERSGAGRPLPAIVRQPVFAVACLGAVT
                     AYAVMNLLMGVTPLAMQMCALPYASAALVIEWHIIGMFAPALIAGSLVHRFGALRVMA
                     AGLLTYLACMLAAVTGQSVLHFWMSGTLLGIGWCLLYVGATTLLTDAYAPAEKAKTQG
                     INDTLVFVVMATSNALSGAILYRYGWNALNYSALPLLLATGIAVGWLALARRALRANT
                     APVPTILE"
     gene            3061..4080
                     /locus_tag="VD791_04290"
     CDS             3061..4080
                     /locus_tag="VD791_04290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXV07222.1"
                     /translation="MTVRLSILDQSPIISGHTPAQAVHATLELAQAADRVGYHRYWLA
                     EHHAMRGLADPCPEILLGQIGARTQRIRIGTGGVLLPYYSALKVAEVFRMLEALFPGR
                     VDLGIGRAPGGGVLTAKAMNAEAFLGEDRFPTQVQEVVGYLDDTLPPEYPYHAVKAMP
                     AGDTSPEVWLLGSSDYSGALAAYLGLRFAFAHFITAHGGDAVMHAYREQFRASYRERR
                     PHSLLAVFAICAPTHEEAERLASSIDLRRYQMTLGIDAPIATVEEAQAYPYPEQARAV
                     IQRERARAVIGTPQAVRERLLALCDQYRADELMVITITGDYGSRLRSYELLAQAFELQ
                     ASASA"
     gene            4200..4730
                     /locus_tag="VD791_04295"
     CDS             4200..4730
                     /locus_tag="VD791_04295"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HXV07223.1"
                     /translation="MKAANWLAAPHLCRPALGICLPALGIWLAALAALALSACGGMTG
                     GDSGPVLVNKKNVGRLQGRQWELKTITVDGTRVIMHPDGVMTIAFAAQGEVGGLGTVN
                     QFRGRYEFSEDGRLTWPAPGLVSTRKAGPPELMDKERAYLAGLPKTSRAIVTKSALQL
                     QSDDGNTVLTFVPLGT"
     gene            4784..5440
                     /locus_tag="VD791_04300"
     CDS             4784..5440
                     /locus_tag="VD791_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HXV07224.1"
                     /translation="MKLVIGNKNYSSWSLRPWVLLTHAGIAFEEVQLKFGDDATVIGV
                     EPWSPTRKVPVLWIDEQPVWDSAAICETVAEIFPEKRLWPAEAAARRVARSVSAEMHS
                     GFADLRKAMPLNIRSSHPDKGRTPGALRDIERIASIWESCRARFGAGGEFLFGGFGCA
                     DAMFAPVTLRFATYAVALPPIAQRYAEAMRALPAMQKWCAGARAETEFVAADEPYATR
                     "
     gene            complement(5467..>5899)
                     /locus_tag="VD791_04305"
     CDS             complement(5467..>5899)
                     /locus_tag="VD791_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV07225.1"
                     /translation="FLIPLLGTIGTLPALVALFLYSLLPIVRNTHSGLDDIAPHLRES
                     ARALGLPAGARLRLIELPLASRSILAGVKTSAVINVGTATIAAFIGAGGYGERIVTGL
                     ALNDNTMLLAGAIPAAVLALLVQGVFELADRHLVPAGLRQA"
BASE COUNT          902 a         2017 c         2036 g          944 t
ORIGIN      
        1 catgcacggc gtacaccggc tccgggaact cgatgtcgtg gctgtccccg gtagcaagcc
       61 gcaccacgcg cagcttcggg aagccgccgc ggcgctcgtg cacgacatag tggtgcgcga
      121 acagatcgac gccgtccagc atcacgtcgt cgcggtgtgg gatcagctcc aaccagccgg
      181 attcgcgcgg gtcgtccacc ggcgcggtca ccaggcggaa gttgcgcccg cggtcgttgg
      241 tgacgatgta gaagcgctcg ccgtgatggt ccaggtagta ctcgtgctcg tgcctgcgcg
      301 ggagcgccag cgcgaaagcg ccgcccggat cgtcggcgcg caaatagcgc acctcggagg
      361 tggtcgcgct gtgcgcggtg aggaacaggt aggcgcggct gcgcgactgg tcgacgccga
      421 tggagaagcg ctcgtcggtc tcctcgtaga gcagctcgtg gcgctcgtcc ccgagccgat
      481 gccggtagag ccggcaggcg cgcttggcct cgtcctcgac cacgtagaac agcgtccggt
      541 ggtcgttgcc ccaggccacc gagcgcgtgt gctccacccg caggggcagc agctcgccgg
      601 tgcgcaggtc cttcacctgc agcgtgtact cgcggaagcc ggtgatgtcg gtcgagaacg
      661 ccagcagatc ggcgtcgccg ctcacgtcgt aggcgcccag cgcgaagaac tcgtgccctt
      721 gcgccagcgc gttcatgtcc agcgtgacct gctcggcgcc gcccgagagc ggccggcggc
      781 acaggatcgg gtagtcctgg ccttgctcgg tgcgcccgta atactcgaat cgcccctcgc
      841 ggtaaggcac gtccatgtcg gtttcccgga tgcgcgccag catctcccga tacagggcct
      901 cccggaacgc ctcggtgtgc gccatcatcg ccgtggtgta ggcgttctcg gcctgcaggt
      961 gcgcacgcac ccgcgggttg tccttgtcgc gcaaccagtg atacgggtcc acgcgcggct
     1021 ggccgtgcac ctgcgtgacg aagggctcgc gcggagcgat gggcggggaa ggcatcggaa
     1081 gtcggcggtc gggagtcgga gcaaggatac aatatgggtt cgcaggcatt cggaggcaag
     1141 gcatgtgggt tcgaattctg gggtcggtgc tggctctggc ggctgcggcg cccaccccgg
     1201 ggcaggacaa gatcgaggcg gcgcaggaca aggtcgaggc gggcagcgtg atatggcacg
     1261 accgccgctg cgcattcttc atcgtgcagt cgtcgtgggg tttcaccctg ttcgagtact
     1321 tgaacggacc ctggccgagc ctcgacgacg tgatcgaagg caagctcgag ggcttcggca
     1381 cgcgcaacgt caccaacaag acggtcgata accagatcac gatggtctac tcggagattt
     1441 cttccaccag caagaaatgg gtgggcggca agatccccgg cttctgccgg cgcaagaagg
     1501 acttcctggc gcagctccaa ggcgaaggcg ccccacccgc tccggcaccg gcgcagcaat
     1561 gaagaacgtc cgctatcgct ccgagttcac gcagttcatc gacgcgtttc tggaacgcaa
     1621 tccgcaggtc gtcgagaagc agaaacgcca ccgcgcgacc tggtgggacc gtccccagga
     1681 cctggccgag cgcgaacgtc aagagcaggc cgatgtcgcg cccaccggct acgcctacta
     1741 tcacaaccct tgagaatggc gaccgccccg gcttcaagtg gcgcggcgcc catgacccgg
     1801 tgactctgat cgacgtcgga ccgacgctgt tccgcgtggt ggacatcgtc gtcaagtgag
     1861 cgcgaagaag aacgtcgccg cgctggccgt gtgccagggg ctgctggtca ccaacaacat
     1921 cgccgtcatc tcgatcggtt cgctggccgg ctacatgctc gcggcgaaca aggccctgtc
     1981 caccctgccg gccaccgcct atatcgccgg cggggcgctg tccacctatc cgatctcgct
     2041 gttcatgaag cgccacgggc ggcgcgccgg gttcacgctc ggggcggcgg ccggcctgct
     2101 gggcgcgctg atctgcacgc tcgcggtggc ggccggcagc tttgccctgt tctgcctggg
     2161 cacgttcatc gcgggcactt ataccgcatc gggcggctac tatcgcttcg ccgccgccga
     2221 tgccgtcggc atggggttca agagccgcgc catctcgctg gtgctggcgg gcggcatcct
     2281 cggcggcatc ctcggcccgg agagcagcaa gctcaccaag gactggcttc cggtgacctt
     2341 catgggttcc tacgccgcgc tgatggcgtt cgcgctgatc gtcatcgcca ttctgcgcct
     2401 ggtccatcta cccatgccct ccgagcagga gcgcagcggc gccggccggc cgctgcccgc
     2461 catcgtgcgc cagccggtgt tcgcggtggc ctgcctgggc gcggtgacgg cctacgcggt
     2521 gatgaacctg ctgatgggcg tcacaccgct cgccatgcag atgtgcgccc tgccgtacgc
     2581 gtctgcggcg ctggtgatcg aatggcacat catcggcatg ttcgcgccgg cgctcatcgc
     2641 cgggtccctg gtgcaccgct tcggcgcgct cagggtgatg gcagcggggc tgctcacgta
     2701 tctcgcgtgc atgctggcgg cggtcaccgg ccagagcgtc ctgcatttct ggatgtcggg
     2761 cacgctgctg ggaatcggct ggtgcctgct gtacgtgggc gccaccacgt tgctcaccga
     2821 cgcctatgct ccggccgaga aggcgaagac gcagggcatc aacgacaccc tggtgttcgt
     2881 cgtcatggcg acctccaacg cgctgtcggg cgcgatcctg tatcgctacg gctggaatgc
     2941 gctcaactac tcggcactgc cgctgctgct cgccacaggt atcgccgtcg gctggctggc
     3001 gctcgcccgc cgggcgttgc gcgcgaacac cgcgccggtg cccaccattc tggagtgagc
     3061 gtgacggtac ggctcagcat cctggatcaa tcgcccatca tcagcgggca cacgccggcg
     3121 caggcggtcc acgccacgct cgagctggcg caggccgccg accgcgtggg ctaccaccgt
     3181 tactggctgg cggagcacca cgccatgcgc ggcctggccg acccctgccc ggagatcctg
     3241 ctcgggcaga tcggcgcacg cacccagcgc attcggatcg gcaccggggg cgtgctactg
     3301 ccttactaca gcgcgctcaa ggtggcggag gtgttccgca tgctcgaggc gctgttcccc
     3361 gggcgcgtgg atctgggcat cggccgcgcg cccggcggcg gcgtgctgac cgccaaggcc
     3421 atgaacgcgg aggcgttcct cggcgaggac cgttttccca cgcaggtgca ggaggtggtg
     3481 gggtatctgg acgacaccct gcctcccgag tatccctatc acgcggtcaa ggccatgccg
     3541 gccggcgaca cttcgcccga agtctggctc ctcggcagct cggactacag cggtgcgctc
     3601 gccgcctatc tcggcctgcg cttcgccttc gcccacttca tcaccgcgca cggcggggac
     3661 gcggtcatgc acgcctaccg cgagcagttc cgggcctcct accgcgagcg ccggccgcac
     3721 agcctgctgg ccgtgttcgc aatctgcgcg cccacgcacg aggaggcgga acggctggcc
     3781 agcagcatcg acctgcgccg ctaccagatg acgttgggca tcgacgcgcc catcgccacc
     3841 gtcgaggagg cgcaggccta tccgtacccc gagcaggcgc gcgcggtgat ccagcgcgag
     3901 cgcgcgcgtg ccgtgatcgg cacgccgcaa gcggtgaggg aacggttgct cgcgctgtgc
     3961 gaccagtacc gggccgacga gctgatggtg atcaccatca ccggcgacta cggcagccgc
     4021 ctgcgctcct atgagctgct ggcgcaggcg ttcgagctgc aggcttcggc atccgcctga
     4081 agccgtgcgc ccttggcctc cggcgcttgt tgcagggcgg cgactcggct aatcttgcgc
     4141 cgcacgaaca tcgggagcgg gtaccccacc cgcttcgcat cgaccgagac gaggatcgaa
     4201 tgaaagcagc aaactggctg gccgccccgc atctctgccg gccggcgctg ggtatctgcc
     4261 tgccggcgct gggtatctgg ctggcggcgc tggctgcgct cgccctgagc gcctgcgggg
     4321 gcatgaccgg cggtgacagc gggccggtcc tggtgaacaa gaagaacgtc gggcgcctgc
     4381 agggccgtca gtgggagctg aagaccatca cggtggacgg cacccgcgtc atcatgcatc
     4441 ccgacggcgt catgactatc gccttcgctg cccagggcga ggtcggcggg ctgggcacgg
     4501 tcaaccagtt ccgcggccgc tacgagtttt ccgaggacgg caggctgacg tggcccgccc
     4561 ccgggctggt cagcacgcgc aaggccggcc cgcccgagct gatggacaag gagcgcgctt
     4621 acctcgccgg cctgcccaag accagccgcg ccatcgtcac caagagcgcg ctgcagctgc
     4681 aaagcgacga cggaaacacg gtgctcacct tcgtgccgct cggcacttga tgcgccctca
     4741 aggcgcgatt tagaaggcaa cctcccgcct gcctatgcct atcttgaagc tcgtcatcgg
     4801 caacaagaat tactcatcct ggtcgctgcg tccctgggtg ctgctcacgc acgccggcat
     4861 tgcgttcgag gaagtgcagc tcaagttcgg cgacgacgcc accgtgatcg gcgtggagcc
     4921 gtggtcgccc acgcgcaagg tgccggtgct gtggatcgac gagcagccgg tgtgggattc
     4981 ggctgccatc tgcgagacgg tggcggagat cttcccggag aagcggctgt ggccggcaga
     5041 ggccgcggcg cggcgcgtgg cgcgctcggt cagcgccgag atgcattccg gcttcgccga
     5101 cttgcgcaag gccatgccgc tcaacatccg ctcctcgcat ccggacaagg ggcgcacgcc
     5161 gggcgccctg cgcgacatcg agcgcatcgc gtcgatctgg gaatcctgcc gcgcgcgctt
     5221 cggcgcgggc ggcgaattcc tgttcggcgg tttcggctgc gcggacgcga tgttcgcgcc
     5281 ggtgaccctg cgcttcgcca cctatgcggt tgcgctgccg ccgatcgcgc agcgctatgc
     5341 cgaagccatg cgcgccctgc ccgcgatgca gaagtggtgc gcgggcgcgc gcgccgaaac
     5401 cgaattcgtc gcggccgacg agccctacgc gacgcgctga tcgggcgctt ctcgctctcg
     5461 gcccggctac gcctgtcgta aaccggccgg caccaggtgg cggtccgcga gctcgaacac
     5521 gccttgcacc agtagcgcca gcaccgccgc gggaatggcg cccgccagca gcatggtgtt
     5581 gtcgttgagg gcgaggccgg tgacgatgcg ctcgccgtag ccgccggcgc cgatgaaggc
     5641 ggcgatggtg gccgtgccga cgttgatcac ggcggaggtc ttgaccccgg cgaggatgga
     5701 gcgcgaagcg agcggcagct cgatcaggcg cagccgtgcc ccggcgggca ggcccagagc
     5761 gcgcgccgat tcgcgcagat gcggggcgat gtcgtcgagg ccgctgtgag tgttgcgcac
     5821 gatcggcagc agcgagtaca ggaacagcgc gaccagcgcc ggcagcgtcc cgatcgtccc
     5881 cagcagcggg atcaggaac
//
LOCUS       DASPGE010000138         2857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_391363, whole genome shotgun sequence.
ACCESSION   DASPGE010000138 DASPGE010000000
VERSION     DASPGE010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391363"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VD791_04310"
     CDS             complement(<1..798)
                     /locus_tag="VD791_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV07226.1"
                     /translation="MPMLQNKVALVTGAGGGIGRDFALAFAAQGARVVVNDLGTSVAG
                     EGASTGPAQKVVDEIRARGGEAVASTESVADWESANRIVKTAIDAFGHLDCVVNNAGI
                     LRDRFFFNMSVEEWRAVIDVHLHGAFYVSRAAAPYFKSQNCGCYVHMTSTSGLIGNLG
                     QANYSAAKLGIVGLSKSIALDMARYKVRSNCIAPFAWSRMIGSIPTETAEQKARVERM
                     KVMETAKIAPLAVFLASDAAQEVSGQIFAVRANELFLMSQSRPIRSVH"
     gene            complement(895..2052)
                     /locus_tag="VD791_04315"
     CDS             complement(895..2052)
                     /locus_tag="VD791_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXV07227.1"
                     /translation="MGNLTIRTQFVALTAACLLLIAALSVVVVSGARSDAAAWIVLAL
                     TVVLLCGVQYWYARRLQKEFARMASAVRSLADGDLTARLESRACVETIALGHEFNGAQ
                     QRLHALLERVQAAARQLLVASGEISQGHADLSARTEQQASSLEEAATAMEQMTATVSQ
                     NAQNAKRVSEHASAAAEVARRGGQAVAGVVGTMMGISDSSGRISDIIAVIDGIAFQTN
                     ILALNAAVEAARAGEQGRGFAVVAAEVRSLAQRSATASREIRQLIQDSVGRIDAGSRE
                     VEEAGETMAEIVTAVKRVHDLVAEISSASQEQSQSLHQVSETVQQLEKVTQQNASMVE
                     QASVVSASMAEQAASLAHALGRFKLQDTVHQGPASRQVMRPPQAAVTWKEV"
     gene            complement(2149..2225)
                     /locus_tag="VD791_04320"
     tRNA            complement(2149..2225)
                     /locus_tag="VD791_04320"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2189..2191),aa:Pro,seq:ggg)
     gene            complement(2241..2624)
                     /locus_tag="VD791_04325"
     CDS             complement(2241..2624)
                     /locus_tag="VD791_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXV07228.1"
                     /translation="MESRSPSKKADLPPIPAKRYFTIGEVSELCGVKPHVLRYWEQEF
                     TQLKPVKRRGNRRYYQHHEVLLIRRIRELLYEEGFTISGARNRLDNAALGAEERAAPA
                     PRPAGGADLGAIRRELRALLDALRP"
     gene            complement(2593..>2857)
                     /locus_tag="VD791_04330"
     CDS             complement(2593..>2857)
                     /locus_tag="VD791_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HXV07229.1"
                     /translation="NKREAKDMVEAFFEEIRGALERGESVKLSGFGNFQLRDKPQRPG
                     RNPKTGEEIPISARRVVTFHASQKLKAMVEKARHGKPQPQQES"
BASE COUNT          420 a         1022 c          941 g          474 t
ORIGIN      
        1 gtgcaccgag cggatcgggc ggctctgcga catgagaaac agctcgttgg cgcgcacggc
       61 gaagatctgg ccggagacct cctgcgcggc atcgctcgcc agaaaaacgg cgagcggcgc
      121 gatcttcgcg gtctccatca ccttcatccg ttccacgcgc gccttctgct ccgcggtctc
      181 ggtggggatc gagccgatca tgcggctcca ggcgaagggc gcgatgcagt tggagcgcac
      241 cttgtagcgc gccatgtcga gcgcgatcga cttcgacagg ccgacgatgc ccagcttggc
      301 cgccgagtag ttggcctgcc cgagattgcc gatgaggccc gaggtcgagg tcatgtgcac
      361 gtagcagccg cagttctggc tcttgaaata cggcgcggcc gcgcgcgaaa cgtagaaggc
      421 gccgtgcaga tgcacgtcga tgaccgcgcg ccactcctcc accgacatat tgaagaagaa
      481 ccggtcgcgc aggatgccgg cgttgttcac cacgcagtcc agatggccga aagcgtcgat
      541 cgccgtcttc acgatgcggt tcgccgactc ccagtcggcc acgctctcag tgctggcgac
      601 ggcctcgcca ccccttgccc ggatctcgtc cacgaccttc tgcgcgggcc ccgtgctcgc
      661 gccttcgccc gcgaccgacg tgcccaggtc gttgaccacg acccgcgcac cctgggcggc
      721 gaaggcgagc gcgaagtccc ggccgatgcc gccgccggcg ccggtcacga gagccacctt
      781 gttctgaagc atgggcatgg ggctcgcgca gtttacctgc ccgcgaccgc agactgggac
      841 tgaggtttta ctggcgcgcc gcgacgcgcg ggcgatgctg cacggcccgg gaccctagac
      901 ttccttccac gtgaccgccg cctgcggtgg gcgcatcacc tggcgactgg ccggcccctg
      961 gtgcacggtg tcctgcagct tgaacctgcc cagcgcgtgc gccagtgacg cggcctgctc
     1021 cgccatggac gcggacacca ccgaggcctg ctcgaccatg gacgcgttct gctgcgtcac
     1081 cttctccagc tgctgcacgg tttcgctgac ctggtgcagg ctctgcgact gctcctggga
     1141 ggccgaggag atttccgcga cgaggtcgtg cacgcgcttc accgcggtca cgatctcggc
     1201 catcgtctcg cccgcctcct ccacctcgcg ggaaccggcg tcgatgcggc cgaccgagtc
     1261 ctggatgagc tggcggatct ccctggacgc cgtcgccgag cgctgtgcga ggctgcggac
     1321 ctccgccgcc accaccgcaa acccccgccc ctgctcgccg gcgcgcgccg cctcgaccgc
     1381 agcgttcagc gccaggatgt tggtctgaaa cgcgatgccg tcgatcacgg cgatgatgtc
     1441 ggaaatcctg ccggacgaat ccgagatgcc catcatcgtg cccacgaccc ccgccaccgc
     1501 ctgcccgccg cgcctggcca cctcggcggc cgcggacgca tgctcgctca cgcgcttcgc
     1561 gttctgcgcg ttctgcgaca ccgtggccgt catctgttcc atggccgtgg ccgcttcctc
     1621 cagcgacgaa gcctgctgct cggtgcgggc agacaggtcg gcgtggccct gcgagatctc
     1681 ccccgaggcg accagcaact gtcgcgcggc ggcttgcacc cgctccagga gcgcgtgcag
     1741 gcgctgctgc gccccgttga actcgtggcc gagggcgatc gtttcgacgc aggcgcgcga
     1801 ttccaggcgg gcagtcaggt cgccgtccgc caggctgcgc acggcgctgg ccatgcgcgc
     1861 gaattccttc tgcaggcggc gtgcatacca gtactggacg ccgcagagca ggaccacagt
     1921 gagcgccagc acgatccacg cggccgcgtc gctgcgcgcg ccgctcacga ccacgacgct
     1981 cagcgcggcg atcagcaaca ggcaggccgc cgtgagcgcc acgaactgcg tgcggatggt
     2041 gaggttgccc atgcggtgca gtagccagcc taagggggcc ctgcagtgcg gtaaatcagc
     2101 gatgtcccta ggggtattcc ccgagggccg atgatccatc cggaagattg gtcggggcgg
     2161 cgagattcga actcgcgacc acctgcaccc catgcaggta cgctaccagg ctgcgctacg
     2221 ccccgaagtg tcagagtcta tcagggccgg agcgcatcca gcagcgcgcg cagctcgcgc
     2281 cggattgcgc cgagatctgc gccgcccgcc ggccgcggcg ccggggcggc gcgctcctcc
     2341 gcgccgagcg cggcattgtc caggcgattg cgcgccccgc tgatggtgaa gccttcctcg
     2401 tacagcaact cgcggatgcg gcggatgagc agcacttcat ggtgctggta gtagcgccgg
     2461 ttgccccggc gcttgaccgg cttcaactgc gtgaactcct gctcccagta gcgcaggacg
     2521 tggggcttca cgccgcacag ctcgctgacc tcaccgatgg tgaagtagcg cttcgccggg
     2581 atcggcggca ggtcagcttt cttgctgggg ctgcggcttt ccatggcgcg ccttctccac
     2641 catcgccttc agcttctggc tggcgtgaaa ggtgaccacg cggcgagcgc tgatgggaat
     2701 ttcctcgccg gtcttggggt tgcgccccgg gcgctgcggc ttgtcccgca gctggaagtt
     2761 gccgaagccg gagagcttga cgctctcgcc ccgctcgagc gcgccccgga tttcctcgaa
     2821 gaacgcctcg accatgtcct tggcctcgcg cttgttc
//
LOCUS       DASPGE010000139         6616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_392064, whole genome shotgun sequence.
ACCESSION   DASPGE010000139 DASPGE010000000
VERSION     DASPGE010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392064"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VD791_04335"
     CDS             complement(<1..1160)
                     /locus_tag="VD791_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXV07230.1"
                     /translation="MRFQVKAVKSATISAMKSDGAVVSLSLDARDDRDADQQARSQGY
                     VVLGVRRTGSLGMPAGLRAARFPLVLFTQELLALLRSGISLVEALETLGEKESKAGTA
                     AVLTGLLARLREGQSFSGALQSHPDAFPDLYVATVRASERTGSLPEALSRFVAYQTQL
                     DAVKKHLVSASIYPLILIAVGGLVSLFLLGYVVPRFSHIYEDIGGDLPWLTRMLMAWG
                     GFVEANGGLLLGAVIAGVAGLLFWVRRPAARQWGARQLMRIPALGERVRIYQLTRFYR
                     TTGMLLMGGTPAPQALTMVSGLLDEELRLRLDAALRRIREGQTMSASLDGQGLATPVA
                     LRMLRVGERTGMMGEMMERSAAFHEEELQRWVERFTRTFEPLLMALIGVVIGG"
     gene            complement(1170..1583)
                     /gene="gspG"
                     /locus_tag="VD791_04340"
     CDS             complement(1170..1583)
                     /gene="gspG"
                     /locus_tag="VD791_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HXV07231.1"
                     /translation="MSYRSNTGFTLLELLVVIVIIGLLAGYVAPRYFAQEGKSKIQIT
                     RAQIDAFEKALDQYRLDTGRYPDTEQGLAALVTRPGDEPKWGGPYLRKAVPNDPWDKP
                     YVYRQPGERGEYDLLSLGKDGQPGGTGEDADIGTF"
     gene            complement(1840..2664)
                     /locus_tag="VD791_04345"
     CDS             complement(1840..2664)
                     /locus_tag="VD791_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07232.1"
                     /translation="MTRLQFSLLALLAALPTCAWAQQPPAGGDLGRQVRELQRQLAGL
                     QSTVRSLRAQLAAEAAARQALQAGMETLTNQLAGLQSDVRGLRGNSILDLNGYLTLDH
                     SSGYPTALFQGINVQVVNGAGTTHSANGLGNLIVGYNRPRAGRPVCSLGYYITASDCR
                     AKGGVWARSHKSGSHNFVGGDFNSYSGWGGVVLGIENAINAPYAAITGGALNVASGDL
                     SGVGGGSGNSATGMYGVVGGGLGNIAAGSFSALSGGARRMAVGSEDWVGGGLLQDQ"
     gene            2828..3982
                     /gene="dinB"
                     /locus_tag="VD791_04350"
     CDS             2828..3982
                     /gene="dinB"
                     /locus_tag="VD791_04350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HXV07233.1"
                     /translation="MDPPVFRRIAHLDMDAFYASVELLRYPELKGRPLVIGGGRRHQP
                     VAGDGERRFARLAGYVGRGVITTATYEARAFGAHSGMGLMKAAALAPDAVLLPADFDQ
                     YRKYSRMFKAAVREIAAQVEDRGIDEIYIDLTGLPGDAREIALRIQERVRSATGLTCS
                     IGVAPNKLLAKICSELNKPNGVTVLSQADVVARIWPLPARRINGIGPKAGSKLDALGI
                     RTIGELAAADRQLLIEQFGRSYGAWMHEAAHGRDERAVVTVSEPKSISRETTFERDLH
                     AVRDRDTLSAIFTDLCVRLSEDLARKGYLGKTIGIKLRYDNFKTVTRDHTIEQPTADA
                     RIIRRAAGECLKRTPLDRRLRLLGVRVGSLVRAATAERAAPPHPSAQLFD"
     gene            complement(4071..4676)
                     /locus_tag="VD791_04355"
     CDS             complement(4071..4676)
                     /locus_tag="VD791_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HXV07234.1"
                     /translation="MPARLWTKRVRAVAIAACLAVPCLGHAQLFKCFDEKGRITYSDA
                     PCMSAVPPRPGPGAVGRINREQALGFMASFDKALARVDFDRVVEHFADDALIQVQVKS
                     QRSGGSGTIGKAEFRRLLREAKDSFWDYRLQRNIVEVRLTGDGSQAEVQSELTEWWKD
                     PGGAMVLKSKETYTIEMRAGRPRATALHVVTRDPEPQVMRQ"
     gene            complement(4792..6027)
                     /locus_tag="VD791_04360"
     CDS             complement(4792..6027)
                     /locus_tag="VD791_04360"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXV07235.1"
                     /translation="MDHGAHAGKARALDGIRVLDLTRILAGPMATQTLADLGAEVIKV
                     ERPGAGDDTRGWGPPFLQDGARRDTRDSTYFLCCNRGKKSITLDLARPEGQHIVRRLA
                     QQCDVLAENFKVGDLARHGLDYAALSRLNPRLVYCSITGYGQSGPYAERAGYDPIAQA
                     MGGMMAVTGERDGPPQRVGVAVVDVLTAHCAVIAILAALRHRDATGQGQHIDMALLDV
                     QVAAMINIAQAYLSADVVAMRNGNLHPSVVPSQSFDCQDGMIMLAAGNDGQFARLCEV
                     LGRDDWARDARYATNAARVRNRAELTGLLQARLAQEPVAHWTERLSRAGVPCGPINGI
                     AQVFRDPQVQHRGLRIEMQHPAAGALPLVASPLRLSATPVDYRLPPPLLGEHTDRVLG
                     DLLGMGQEEIGMLRRARVV"
     gene            complement(6032..>6616)
                     /locus_tag="VD791_04365"
     CDS             complement(6032..>6616)
                     /locus_tag="VD791_04365"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXV07236.1"
                     /translation="VHPSLPASDLKALVALAKARPGELTIASQGTGTVSHLEAEMLQQ
                     MAGIALTHVPYKGSAPALVDLLGGRTQIMFDSIASAMPQVRAGKLRALAVAAEKRASV
                     LPDVPTVAEAALPGYRAESWLGILAPARTPRAVVERLNRELNALLAEARVRQTLIERG
                     FEPQASTPEQFVERIRHDLLAWAKVVKASGATVE"
BASE COUNT         1010 a         2371 c         2234 g         1001 t
ORIGIN      
        1 cctccgatca ccacgccgat cagcgccatc agcagcggct cgaaggtgcg ggtgaagcgc
       61 tcgacccagc gctgcagctc ctcttcgtgg aacgcggcgc tgcgctccat catctcgccc
      121 atcatgccgg tgcgctcgcc cacgcgcagc atgcgcagcg ccaccggcgt ggcgagcccc
      181 tgcccgtcaa gcgacgcgga catcgtctgt ccctcgcgga tgcggcgcaa ggcggcgtcc
      241 agccgcagac gcagctcctc gtcgagcaga cccgacacca tggtcagcgc ctggggcgcc
      301 ggcgtgccgc ccatcagcaa catccccgtc gtgcggtaga agcgcgtgag ctggtagatg
      361 cgcacacgct cgccgagcgc cgggatgcgc atcagctggc gagcgcccca ttggcgcgcg
      421 gccggccggc gcacccagaa cagcaggccc gccaccccag cgatgacggc gcccagcagc
      481 agcccaccgt ttgcctcgac gaagccgccc caggccatca gcatgcgcgt cagccagggc
      541 aggtcaccgc cgatgtcctc gtagatgtgg gagaagcgcg gcaccacgta gcccagcagg
      601 aacaggctca ccaatccgcc gaccgcgatc aggatcagcg gatagatcga ggcgctgacc
      661 aggtgcttct tcaccgcgtc cagctgtgtc tgataggcga cgaagcgcga cagggcttcg
      721 ggcaggctgc cggtacgctc gctggcgcgc accgtggcca cgtacaaatc gggaaaagca
      781 tccggatggg actgcagcgc gccggagaag gactgccctt cgcgcaggcg cgccagcaac
      841 ccggtcagaa cggccgcggt gccggccttc gattccttct cgccgagcgt ctccagcgcc
      901 tcgaccaggc tgatgccgga gcgcagcagg gccagcaact cctgcgtgaa caggaccaga
      961 ggaaagcgtg ccgcgcggag gcccgccggc atgccgagcg atcccgtgcg ccgcaccccg
     1021 agcacgacgt agccctggga tcgtgcctgc tggtcggcgt cgcgatcgtc gcgcgcgtcg
     1081 agcgacaggg acaccaccgc gccatcggat ttcatggcgg agatcgtggc ggatttcacc
     1141 gctttgacct ggaagcgcat ggcctgaagt cagaaagtcc cgatgtccgc atcctctccg
     1201 gtgcctccgg gctggccgtc cttgcccaga gacagcagat cgtactcgcc ccgttcgccc
     1261 ggctggcggt acacgtaggg tttgtcccag ggatcgttgg ggaccgcctt gcgcagatag
     1321 ggaccgcccc acttcggctc gtcgccgggg cgcgtcacca gcgctgctag cccctgctcg
     1381 gtgtccgggt agcggcccgt gtcgagccgg tactggtcga gcgccttctc gaacgcgtcg
     1441 atctgcgcgc gcgtgatctg gatcttggat ttgccctcct gcgcgaagta gcgcggggcg
     1501 acgtagccgg cgagcaggcc gatgatcacg atcaccacca gcagctcgag cagggtgaaa
     1561 ccggtgttgc ttcggtagga catgagcgcc attctcgtgg tcggtgttgc gtgcgcttgc
     1621 gccccgctcc aggctggcgc gcggccggag ccccccaggc aagcgcgtcc attgtacggc
     1681 ggcagtctag gtcccgcccg tgacgccgcc gttacttcga tcttggttgt aacaccgggt
     1741 tcacgggagc gtcaagccgg ggcccgtcgt caagccgggg tccgtcattg tgaaattcgc
     1801 gctgcagcgg cccgctgtcg ccggaacaaa acaaaccggt cactgatcct ggagcagccc
     1861 gccgcccacc cagtcctccg acccgacggc catgcgccgc gccccgccgc tgagggcgga
     1921 gaaagacccg gccgcgatgt tgcccagacc gccgcccacc acgccgtaca tgccggtggc
     1981 gctgttgccg ctgccgccgc cgacgcccga cagatccccg ctcgcgacgt tgagcgcacc
     2041 gccggtgatg gcggcatagg gtgcgttgat ggcgttttcg atgcccagga cgacgccgcc
     2101 ccaacccgag tagctgttga agtcgccgcc gacgaagttg tgcgagccgc tcttgtgact
     2161 gcgcgcccag acgccaccct tggcacggca atccgatgcg gtgatgtaat agccgagcga
     2221 gcagaccggg cggccggcgc gcggccggtt gtagcccacg atcaggttgc ccagaccgtt
     2281 ggcgctgtgc gtggtaccgg cgccgttgac cacctgcacg ttgatcccct ggaacagcgc
     2341 ggtcggatag ccgctcgagt gatccagggt gagatagccg ttcaagtcga ggatgctgtt
     2401 cccgcgcaag ccgcgcacgt cgctctgcag gcccgcgagc tggttcgtca gcgtctccat
     2461 gccggcctgc agcgcctggc gcgcggcggc ttccgccgcg agctgcgcgc gcaggctcct
     2521 gaccgtgctc tgcaggccgg cgagctggcg ctgcagctcg cgcacttggc ggccgaggtc
     2581 gccgcccgca ggcggctgct gagcccaggc gcaggtgggc agcgcagcga gcagcgcgag
     2641 cagcgaaaac tggagtctgg tcatgtcggc gttccccatg aatcggtggc ttcgccagtt
     2701 tacgccgaca ccgggttcgc gccgacaccg ggttcgcgcc gacaccgggt tcacgccaac
     2761 accggggcac gacgcagtgt ttcgagagaa aatgctgctc tcgattcgcc cccgcttccg
     2821 agcagtcgtg gacccgcccg tcttccgccg catcgcccac ctggacatgg acgcgttcta
     2881 cgcgtcggtg gagctgctgc gctatcccga gctgaagggg cggcccctgg tgatcggcgg
     2941 cggccggcgg caccagccgg tcgccgggga cggcgagcgc cggttcgcgc ggctcgcagg
     3001 ctacgtgggg cgaggcgtga tcacgaccgc cacctacgag gcgcgcgctt tcggcgcgca
     3061 ctcgggaatg ggcttgatga aagcggcggc gctcgcgccc gatgcggtgc tgctgcccgc
     3121 cgacttcgat cagtaccgca agtattcgcg catgttcaag gcggcggtgc gcgagatcgc
     3181 cgcgcaagtc gaggatcgcg gtatcgacga gatctacatc gatctcaccg gcctgccggg
     3241 agacgcgcgt gagatcgcgc tgcgcatcca ggagcgcgtg cgcagcgcca ccggcctgac
     3301 ctgctccatc ggcgtcgcgc ccaacaagct cctggccaag atctgctcgg agttgaacaa
     3361 gcccaacggc gtgaccgtgt tatcgcaggc cgacgtcgta gcccggatct ggccgctgcc
     3421 ggcgcgacgc atcaacggca tcgggcccaa ggccggcagc aagctggatg cactgggcat
     3481 ccgcaccatc ggggagctgg cagcggccga tcgtcagttg ctcatcgagc agttcggccg
     3541 cagctacggc gcctggatgc acgaggcggc gcacgggcgc gacgagcgcg cggtggtgac
     3601 ggtgagcgaa ccgaagtcga tcagccggga aaccacgttc gagcgcgacc tgcacgcggt
     3661 gcgcgatcga gacaccctga gcgcgatctt caccgacctg tgcgttcgcc tgagcgagga
     3721 tctggcacgc aaggggtatc tcggcaagac catcggtatc aagttgcgct acgacaactt
     3781 caagacggtc acgcgcgatc acaccatcga gcagccgacc gccgatgcgc gcatcatccg
     3841 ccgcgccgcc ggcgaatgcc tgaagcgcac gccgctggac cggaggctgc ggctgctcgg
     3901 ggtgagggtg ggatcgctgg tgcgggcggc gaccgcggag cgggctgcgc cgccgcaccc
     3961 gagcgcgcag ctgttcgact aagaacttcg ccggacgcgg cgcgcaagcc tccgcactac
     4021 cccccgacat ctggtagggt gtttaagtcg cgccttcagg gcgcaatcgg ctactgacgc
     4081 atcacctgcg gctccgggtc gcgggtgacg acgtgcaacg ccgtcgccct gggcctgccg
     4141 gcgcgcatct cgatggtgta ggtctccttg gacttgagta ccatggcgcc gccgggatcc
     4201 ttccaccatt cggtgagctc ggactggacc tcggcctggc tgccgtcgcc ggtgagcctc
     4261 acctccacga tgttgcgctg cagccgatag tcccagaagc tgtccttcgc ctctcgcaac
     4321 aggcggcgga actccgcttt gccgatggtt ccggagcccc cggagcgttg cgatttcacc
     4381 tgcacctgga tcagcgcgtc gtcggcgaaa tgctcgacga cccggtcgaa atccacccgg
     4441 gccagcgcct tgtcgaagct cgccatgaag cccagcgcct gctcgcggtt gatcctgccc
     4501 accgcgccgg gaccgggacg cggcggtacg gccgacatgc aaggggcgtc ggaataggtg
     4561 atccggccct tctcgtcgaa gcacttgaac agctgggcat ggcccaggca cggcactgca
     4621 aggcaggcgg cgatggcaac ggcgcggacc ctcttggtcc acagtctggc cggcatgacg
     4681 ctctccttcg ggatgcggct gcccgccaga atcgcaagcc tcataccaat ccaggccgtc
     4741 cggggccctg cccccgggcc cttctgtgac atttgcggcg tttctttgga gctatacgac
     4801 gcgcgcgcgc cgcagcatcc cgatctcctc ctgccccatt ccgagcagat cgcccagcac
     4861 gcggtcggta tgttccccca ggagcggggg cggcaggcga tagtccaccg gcgtcgccga
     4921 caggcgcagc gggctcgcca ccagcggcag cgcgcccgcc gcggggtgct gcatctcaat
     4981 gcgcaggccc cggtgctgca cctgcggatc gcggaacacc tgcgcgatgc cgttgatcgg
     5041 cccgcagggg acgccggcgc gggacaggcg ttccgtccag tgcgccacgg gctcctgcgc
     5101 cagccgcgcc tgcaacagcc cggtcagctc ggcccggttg cgcacacgcg ccgcgttggt
     5161 ggcgtagcgc gcgtcgcgcg cccagtcgtc gcgccccaac acctcgcaca ggcgtgcgaa
     5221 ctgcccgtcg ttgcccgcgg ccagcatgat catcccgtcc tggcagtcga acgactgcga
     5281 cggcaccacg ctcgggtgca ggttgccgtt gcgcatcgcc accacatcgg cggagagata
     5341 cgcctgggcg atgttgatca tggcggccac ctgcacgtcg agcagcgcca tgtcgatgtg
     5401 ctgcccctgc ccggtcgcgt cgcggtggcg cagcgccgcc aggatcgcga tgaccgcaca
     5461 gtgggcggtg agcacgtcca caaccgccac gcccacccgc tgcggcggac cgtcgcgctc
     5521 gccggtgacc gccatcatcc cgcccatcgc ctgggcgatg gggtcgtagc ccgcgcgctc
     5581 ggcgtacggt ccgctctggc cgtagccggt gatggagcaa tacaccaggc gcggattcag
     5641 acggctgagc gcggcgtagt ccagcccgtg gcgcgccagg tcgcccacct tgaagttctc
     5701 ggcgagcacg tcgcactgct gcgccaggcg gcgcacgatg tgctggccct cggggcgcgc
     5761 caggtccaag gtgatggatt tcttgccgcg attgcagcac aggaagtagg tgctgtcgcg
     5821 cgtgtcccgc cgcgcgccat cctgcaggaa cggcggcccc cagccgcgcg tatcgtcgcc
     5881 cgcgcccggg cgctcgacct tgatgacttc ggcgcccagg tcggccagcg tctgcgtggc
     5941 catcggacca gccaggatgc gcgtgaggtc caggacgcgg atgccatcca gggcccgcgc
     6001 cttccccgcg tgtgcgccgt gatccaacgc ctcactccac cgtcgcgccg gaggccttca
     6061 ccaccttggc ccaggcgagc aggtcgtggc ggatgcgttc cacgaactgc tccggggtgc
     6121 tcgcctgcgg ctcgaagccg cgctcgatca gcgtctggcg caccctggcc tcggcgagca
     6181 gcgcgttcag ctcgcgattc aagcgttcga ccacggcgcg cggcgtgcgc gccggcgcca
     6241 ggatgccgag ccaggattcc gcgcgatagc cgggcagcgc cgcctccgcc accgtcggca
     6301 cgtccggcag cacgctcgcg cgcttctccg cggccacagc cagcgcccgc agcttgcccg
     6361 cgcgcacctg cggcatggcc gaggcgatgc tgtcgaacat gatctgcgtg cggccgccca
     6421 gcagatccac cagcgcgggc gcgcttccct tgtacggaac gtgcgtcagc gcgatgcccg
     6481 ccatctgctg cagcatctcg gcctcgaggt gcgacaccgt gccggtgccc tgcgaggcga
     6541 tcgtcagctc gcccggcctg gccttggcca gcgccaccag cgccttcaga tcgctcgccg
     6601 gcagcgacgg gtgcac
//
LOCUS       DASPGE010000140        16194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_861174, whole genome shotgun sequence.
ACCESSION   DASPGE010000140 DASPGE010000000
VERSION     DASPGE010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16194
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861174"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VD791_04370"
     CDS             <1..956
                     /locus_tag="VD791_04370"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXV07237.1"
                     /translation="SAAFDNAIAVLMALGGSTNAIVHLIAMAGRAGIRLDLARFDEIS
                     RRVPLLANLRPSGKYLMEDFYYAGGLLGLMAEMKSLLDLSQRCVNGATLGENIDGAKS
                     YNHDVIATLERPVQSSGGLAVLRGNLAPDGAVIKPSAAEPKLLQHTGKAVVFEDYDDM
                     AKRIDDEALAVEASSVLVLRNAGPLGGPGMPEWGMLPIPKKLLKQGVRDMVRISDARM
                     SGTSYGACVLHVAPESFVGGPLALVRTGDPIELNVPARRLDLKVSDAELHRRRAAWTA
                     PQPKYRRGYGVLFSQHITQANEGCDFRFLEGTAPTPEPEIH"
     gene            1086..2075
                     /locus_tag="VD791_04375"
     CDS             1086..2075
                     /locus_tag="VD791_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07238.1"
                     /translation="MWCAASCFLAALALGGCAMFRSDADLASLQAYRLHTVELPVGNG
                     TVVIAHPRRALALDEADLQGWVSDAARAVHTYYGRLPVPKLQVVVDATDGRGAQSGTT
                     FGFSTPVIRMTLGRATQGEDLAHDWMMTHEMVHLAFPRLAQMHHWLEEGLATYVEPIA
                     RAQAGQLPAEVVWRDLVHGLPLGLPQPGDRGLDYTPSWGRTYWGGALFCLLADIEIRK
                     RTDNRYGLQDALRGVVDAGGTIFVLWSVEHALEVADRSVGVDVLTRLYAEMRTTPVHV
                     DLAELWRQLGVSVRDDDIAFDDDAPLAALRKAITAAPPPPSPFGGPGREGGRL"
     gene            2089..2889
                     /locus_tag="VD791_04380"
     CDS             2089..2889
                     /locus_tag="VD791_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV07239.1"
                     /translation="MSAATPQRIVCLTEEPTEVLYALGEEQRIVGISGYTVRPARARR
                     EKPKVSAFTSAKTDEILALQPDLAIGFSDIQADIAQALIKQGVEVWISNHRSVEGILG
                     YVRRLGALVGACDEAEGYARELEAHVASVRERASRLTRRPRVYFEEWDEPQISCIRWV
                     SELIGIAGGQDVFPERAAAPLARARILADPQEVVQRAPDIILGSWCGKKFRPERVAAR
                     AGWAAIPAVRDGELHEIKSSIILQPGPAALTDGLDALHAVIARWAART"
     gene            complement(3000..6002)
                     /locus_tag="VD791_04385"
     CDS             complement(3000..6002)
                     /locus_tag="VD791_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXV07240.1"
                     /translation="MTSVAQPVQFVRRDVAPAHPLEARLRREVDGEVLFDAFTRGRYS
                     TDASIYQIEPIGVVVPRTEEAARAAAEIAIEAGVPLLPRGAGTSQCGQTVGAALVIDY
                     SKHLNAVAAVDAERREAEVQPGVVLDRLNADLRRHGLWYPVDVSTSAQATLGGMAGNN
                     SCGSRSIRYGNMVHNVLGIEAWLPSGECYWFGPEAELPSGPPGYRALIEKVHAIVRRE
                     AEEIAARWPTVLRRVQGYNLDMVQPHLPHNLAHLLVGSEGTLACFRRLRLKLSPIPRH
                     KALGACHFPTFHQAMDLARHIVPLEPDAVELVDRTMLDLAHEIPQYRDVLRQFVRGAP
                     GAVLLVEFAGEERDAQIARLKRLSELMADLGLPGSVVEVTDAKLQKDIWEVRKAGLNI
                     MMSMKGDGKPVSFIEDCAVPLEHLAEYTHRINEIFERHGTQGTWYAHASVGTLHVRPV
                     LNMKEDGAAKMRAIAEEACALVQRFKGAYSGEHGDGLVRSEWIEPILGARLTAALAEV
                     KALFDPRGLMNPGKIVQPKKMDDRELFRFKPGYATRPLDTALDWSEWGGFAGAVEMCN
                     NNGHCRKFDAGTMCPSYRTTLEEQHLTRGRANSLRLALSGQLGGEALTSDEMYATLDL
                     CVGCKGCKRECPTGVDMARMKVEFLHHYHRRRGYGLRDKLIAYLPRYAPWAARLAPLV
                     NLRDRVPALARLSELWLGLSARRALPRWRQDTFLGVSSALSPPHPTLSREGRGTSWSA
                     GEGVSSSPASEQLDRPVVLFADTFNNYFEPDNLRAAVRVLAAGGYRVEIARPADGGRP
                     LCCGRTFLALGMVDEARREARRVIEALKPHAERGVPVVGLEPSCLLTLRDEYLVLGLD
                     DEEVGVVAERADLFEEFLAGELDAGRLRLPLQPIPDGRALLHGHCHQKAFAAMPAVER
                     VLQLIPGLQVESVESSCCGMAGAFGYEAAHYDISMKMGEASLLPKVRSASPSTLIVAD
                     GTSCRHQIRDAAGREALHVARVLQRALG"
     gene            6085..6684
                     /locus_tag="VD791_04390"
     CDS             6085..6684
                     /locus_tag="VD791_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07241.1"
                     /translation="MPRPTRALWFLLCCLPASALAIDNAWVRVSRDAAPCAVATAPDC
                     GYRVLVALAELDLRAGDASKRLVRGDIAVFGPGQDYTAPSGSYFEVAIKPGHPPALAP
                     AEIIAAEKNAPLYDGEDFFVFEERLAPGDTRARHSHSQRVVIQLNKARLQQWPDGAPE
                     LFVETVPERPNFSPPVIHVVKNVGEVPLRGIVIEFKPGR"
     gene            6689..7885
                     /locus_tag="VD791_04395"
     CDS             6689..7885
                     /locus_tag="VD791_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXV07242.1"
                     /translation="MNRRSEIPSGAALHTGRHFLQIPGPTNVPDRVLRAIDQPTIDHR
                     GPEFAELGKEVLAGMKQVFKTAHPVVIYPASGTGAWEAALVNTLSPGDHVLMAETGHF
                     AALWRRMAERLGLQVEFLPGDWRHGADSAAIEARLVQDGVHRIKAVAVVHNETSTGCV
                     TRVPEVRRALDHAAHPALLMVDTISSLASIDYRMDEWGVDVTVGGSQKGLMLPPGLSF
                     NAVGPKALAAAKRATLPRSYWSWDEMLAANAGGFFPYTPATNLLFGLREALRMLREEG
                     LENVFARHQRHAEATRRAVAGWGLEVLCADPREYSASLTAVLMPEGVDADRVRQIVLQ
                     RFDMSLGTGLGKLQGKIFRIGHLGHFNDLMLAGTLCGVEMGLQLAGVPLRGSGVKAAL
                     EHLAQA"
     gene            complement(7851..9110)
                     /locus_tag="VD791_04400"
     CDS             complement(7851..9110)
                     /locus_tag="VD791_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HXV07243.1"
                     /translation="MERRKHPAYDRLLMGSPLRRRSVAVLVGATLLVLLAVSTLIHLT
                     LLAAMRPEVAMIYYRALGLSFLLALVPLAVLWFLDRRERETPWLFAAAFLWGGCIATA
                     LAMPLNTAFFIAVDAWVAQHPAITEVLGTDAAMMLAAPISAPIAEETTKALGLALLFW
                     LLRAEFDNMRDGLVYGALVGLGFTWLEAALYVAKSYVEQGAVSYGWQLGLRYALFGFG
                     GHALFTGIFGACLGLALQTRRRWLRILAPILGLGLAVATHMLNNALPLFAALAGAASG
                     EPPAGPALDMAFLDAFLAGTLTQLTIFLPFVAIMALVLWRSGVWERRVIREELANEIG
                     LAVTPAEYGEIVRDRALRTRRIDRMRPRASSALVNAQHELAFRKRRVRDQGQDPERDP
                     LVAGWREDIRRLREAAFTPARGAPAPL"
     gene            complement(9163..9951)
                     /locus_tag="VD791_04405"
     CDS             complement(9163..9951)
                     /locus_tag="VD791_04405"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HXV07244.1"
                     /translation="MSTTQPSDLPGGSILSTVAAAIIGVSMGLTSHTAQSTQPTASGF
                     GVLVFSRTAGYRHDSIAAGVAAVNGLGEKHGFRVDATEAAVAFTDENLRRYRVVIFLN
                     TTGDVLDADQQAAFERFIRRAGGFVGVHSASDTEYDWAWYGGLVGAYFKSHPRIQPAT
                     LKVVDATHPSTLHLPVEWKRTDEWYNFREDPAAHGRALLVIDERSYSGGTMRAHHPMA
                     WCQEYDGGRSWYTALGHTIETYSEPLFLEHLLGGIRWAAGVAAE"
     gene            10075..12345
                     /locus_tag="VD791_04410"
     CDS             10075..12345
                     /locus_tag="VD791_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07245.1"
                     /translation="MGDDTHGKSVLQLQTRGGFLARAVLLAFFSFLTVGLGNIIIVGV
                     DPARETVEAFNLGKGLSQLLCFWVALFYWALCAWYTARLLLEKSFAPFDPLLPCVSER
                     FARGLIKWAPRGLGVAAIVPIALVFLIAQGMKHTPLWVRISPIGIAAVFFGFVVFRRK
                     FFGDLVELLPQEPGYGYRRFSRINWKGWLLISVLGAISFSTLLLLWIDGVAVARWVGA
                     PALVLIALGSWSIFGGFVLIYLPMSLKWPALTLWPFVLAALFSLWMENHNVPVGPPPK
                     AGAAPAEWVRPTLDEHWRQWRAALDATPCKDKAIYLVSIAGGATRAAFWGAHTLAALE
                     HHERTLAAAEKRAPCFARSTFAISGVSGGALGAATFVSLLAQEQAVQHQWGELLPLAA
                     ADFLGRDVLAPVLGYMLFQDGLQRFLPYPFASLDRSHGLEDAWISDWADMVQELSPEE
                     MHRGGAPNWFARPMRDLYADGRGTTLPSLFLNTTRVADGKKVVQSNLRFVPNDAYDLY
                     ARGMLTGELTLAGAVHNSARFTYVSPAGRAWRWDEMRGRSTPWGYVVDGGYFENSGSA
                     TLAPLIREIGRLDPDNARRLVLIMLSNDPSDGATDYVCEDPAPQLEPKRAGPRESSPR
                     FLRERLRKPGIFAMEVAAPAIALYQTRASRAHAADLQAKNAVDRECASGRIVELRIPI
                     PASATDDPPMSWFLNEPTIQGLTQRITESAAAADTPARRLCENVELLKWRIQTPVADT
                     SPRLLLEHCRQRPPAE"
     gene            complement(12347..13228)
                     /locus_tag="VD791_04415"
     CDS             complement(12347..13228)
                     /locus_tag="VD791_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXV07246.1"
                     /translation="MLKRADAGDLSVAYEEDGSATGAPILLLHGFPYDVHAYDEVTPI
                     LVSAGCRVITPYLRGYGPTRFRSPDTLRSGQQAVLAHDLLVLMDALAISRAVLAGYDW
                     GGRAACIVAALWPERVRGLVSGGGYNIQDITASGRPAAPENELRYWYQYYFHGERGRA
                     GLEQNRRALCKLLWQLWSPNWRFGDVTFERSAASFDNPDFVPVVIHSYRHRFGLVPGD
                     PNVEDTERRLAMQPRIAVPSIALDGGGDGVMPEGGSEKHGRFFTGRYERRVIPLVGHN
                     LPQEAPKEFAEAVLAVA"
     gene            complement(13241..14086)
                     /locus_tag="VD791_04420"
     CDS             complement(13241..14086)
                     /locus_tag="VD791_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HXV07247.1"
                     /translation="MKFSVQTAVLPELTREQVVEKLRAHGYDGVEWRVHEDYHVAPKE
                     LVARARDIKRLVDAHGLEVSCLTGYAPIGDVAQQRWFAEACAIMGCPRYRPGAVIYDG
                     SRNYGELYQATVDALGKAIEAIAAFAVKPVIETHFGTIAPSAGLAYRLVQHFDPARIG
                     VNYDPANLIIEGRESWQMGLELLGPYLDYVHAKNVAWVREKGAWRWVFASMADGQVDW
                     AELMRLLKRVGYDGYVSFENFHLVPMKSKGFVGEDLTQHAEVFRDIDRRLDEDLRYLK
                     GLASA"
     gene            14253..15218
                     /locus_tag="VD791_04425"
     CDS             14253..15218
                     /locus_tag="VD791_04425"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXV07248.1"
                     /translation="MSKPRHFEPKGVIPACLLPFKEDLSIDARAFRKHLRHVAAVPGL
                     SALTINAHSTEVSSCSFEEQQVVLEIALDELGDRIPLVNGIYADGSLEAARLARMAAQ
                     AGASCLLVFPPNGLGLGARERPEMVIAHFKAIADATDLPLIAFEYRLESGLGYPLDTL
                     LQLADQVPTVRAIKDWCNNVPLHERHIRVLQNLPRPVNVLTTHSAWLLSSLVLGCKGL
                     LSGAGSVIADLQAELFQAVQGNGLARAREINDRIFPLTEVFYADPFVDMHNRMKEALV
                     ILGRIPCAAVRPPLIKLSEAEVARIRGALQQARLLPERKRTAAAA"
     gene            complement(15255..>16194)
                     /locus_tag="VD791_04430"
     CDS             complement(15255..>16194)
                     /locus_tag="VD791_04430"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HXV07249.1"
                     /translation="TETVYGLGADASKRKAVKRIFEAKGRPATHPLIVHLKGSGDLER
                     WAREIPDAARQLAERFWPGPLTLVLKRAPKVLDVVTGGQDTVGLRVPSHPVAQALLQA
                     FGGGIAAPSANRFGRVSATTAQHVREELGERVDLILDGGACPVGIESTILDLSTGAPV
                     LLRPGHITAEEIGALLHAPVTRGGAASPRAPGGLAAHYAPQLPLRLVPRPMLDATVRE
                     QAAHGPVAVLARHSRPTSSAATVWMHAPLDAASYAQHLYAGLRQLDRSGCVAILVEAP
                     PETADWAAVRDRLTRAARGSGAADRIPVSSDASDGT"
BASE COUNT         2533 a         5619 c         5545 g         2497 t
ORIGIN      
        1 cctctgcggc cttcgacaac gccatcgccg tgctgatggc gctcggcgga tcgaccaacg
       61 ccatcgtgca tctcatcgcc atggccggcc gcgccggcat caggctcgac ctggcccgct
      121 tcgacgagat ctcgcgccgc gtcccgttgc tcgccaacct gcgcccctcg ggcaagtacc
      181 tgatggagga cttctattac gcgggcgggc tgctcgggct gatggccgag atgaagagct
      241 tgctcgacct gtctcagcgc tgcgtcaacg gcgccactct gggcgagaat atcgacggtg
      301 cgaagagcta caatcacgat gtgatcgcca cgctggagcg gccggtgcag tcgagcggcg
      361 ggctggcggt gctgcgcggc aacttggcgc ccgatggcgc ggtcatcaag ccgagcgccg
      421 ccgagccgaa attgctacaa cacaccggca aggcggtggt gttcgaggac tacgacgaca
      481 tggcaaagcg catcgacgac gaagccctgg cggtggaggc ctcgtccgtg ctggtgctgc
      541 gcaatgccgg gccgctgggc ggtcccggca tgccggagtg gggcatgctg ccgatcccga
      601 agaagctgct gaagcagggc gtgcgcgaca tggtgcgcat ctccgatgcg cgcatgagcg
      661 gcaccagcta cggcgcctgc gtactgcacg tcgcgccgga gagtttcgtc ggcggcccgc
      721 tggcgctggt gcgcaccggt gatccgatcg agctgaacgt gccggcgcgc cgcctggacc
      781 tgaaggtgag cgacgccgag ctgcaccgcc ggcgtgcggc atggacggcg ccgcaaccga
      841 agtaccggcg cggctatggg gtgctgttct cgcagcacat cacgcaggcg aacgagggct
      901 gcgatttccg cttcctcgag ggcaccgcgc cgacgcccga acccgagatc cactagcctg
      961 tgcgcatcgc tgaaccaccc cgtcgcgctt cgcgcgccac ccctctgggt gcgccctcaa
     1021 ggcgcgactt aaaaacctgc tccccgcctc tcacgaggcg gggtggcgcc gcaggcgccg
     1081 gggcggtgtg gtgtgctgca tcctgcttcc tcgcggcgct ggcgctgggc ggctgcgcca
     1141 tgtttcggtc cgacgccgac ctcgcgtcgc tgcaggccta tcgcctgcac accgtcgagc
     1201 tgccggtggg caacggcacc gtggtgatcg ctcacccacg gcgagcgctc gcgctggatg
     1261 aagccgatct acagggctgg gtgtccgacg cggcgcgcgc ggtgcacacc tactacggcc
     1321 ggctgccggt gccgaagctg caggtggtgg tggacgccac cgacgggcgc ggggcgcagt
     1381 cgggcaccac gttcggcttc agcacgccgg tgatccgcat gacgctgggc cgcgcgacgc
     1441 agggcgagga cctcgcgcac gactggatga tgacgcacga gatggtgcac ctcgccttcc
     1501 cgcggctggc gcagatgcac cactggctgg aggagggcct cgccacctac gtcgagccga
     1561 tcgcgcgcgc gcaggcgggg cagcttccgg cggaagtggt ctggcgcgac ctggtgcatg
     1621 gcctgccgct cggcctgccg caacccggcg accgcggcct ggactacacg ccgagctggg
     1681 gtcgcaccta ctggggcggc gcgctgttct gcctgctcgc cgacatcgag atccgcaagc
     1741 ggaccgacaa ccgctatggc ctgcaggatg cgctgcgcgg cgtggtggac gccggcggca
     1801 ccatcttcgt gctgtggtcg gtcgagcacg cgctggaggt ggcggaccgt tcggtcggcg
     1861 tggacgtgct gacccggctg tacgcggaaa tgcgcaccac gcccgtgcat gtggacctgg
     1921 cggagctgtg gcgccagctc ggcgtgagcg tgcgcgacga cgacatcgcg ttcgacgacg
     1981 atgcgccgct cgcggcgctg cgcaaggcga tcaccgcggc gccgccgccg ccttcgccgt
     2041 tcggcggtcc cggccgcgag ggagggcgac tctgagcgcc ggcgccggat gtccgcagcc
     2101 actccgcaac gcatcgtctg cctgaccgag gagccgaccg aggtgctgta cgccctcggc
     2161 gaggaacagc gcatcgtcgg catctcgggc tacaccgtgc gaccggcgcg agcgcgccgc
     2221 gaaaaaccga aggtatcggc cttcaccagc gccaagaccg acgagatcct ggcgctgcag
     2281 ccggatctcg cgatcggctt ctccgacatc caggcggaca tcgcccaggc tctgatcaag
     2341 cagggcgtcg aggtctggat cagcaaccac cgcagcgtgg aaggtatcct cggctacgtg
     2401 cgccggctcg gcgcgctggt gggcgcctgc gacgaagccg agggctacgc gcgcgagctc
     2461 gaagcgcatg tggcgtcggt gcgcgagcgc gcatcccggc tgacgcggcg cccgcgggtc
     2521 tatttcgagg aatgggacga gccgcagatc agctgcatcc gctgggtcag cgagctgatc
     2581 gggatcgctg gcggacagga tgtgttcccg gaacgggccg cagcccctct cgcacgcgcg
     2641 cgcatcctgg ccgatccgca ggaagtggtg cagcgcgcgc cggacatcat cctcggctcc
     2701 tggtgcggca agaagttccg gccggagcgg gtggcggcac gcgccggctg ggcggccatc
     2761 cccgcggtgc gcgacggcga gctgcacgag atcaagtcct cgatcatcct gcagcccggc
     2821 ccggccgcgc tcacggacgg cttggatgcg ctgcacgcgg taatcgcgcg ctgggcggcg
     2881 agaacataac accacaccgc cccggcgcct gcggcgccac cccgcctcgg agaggcgggg
     2941 agttgtcagt tcccctcttc gccgaggagg ggtggcgcgc gatagcgcga cggggtggtt
     3001 cagcccaatg cccgctgcag cacgcgcgcg acgtgcagcg cctcgcgccc cgcggcatcg
     3061 cggatctgat ggcggcagct ggtgccgtcg gcgacgatca aggtcgaggg cgaggcgctc
     3121 cgcactttcg gcagcaggct cgcctcgccc atcttcatcg agatgtcgta gtgcgccgct
     3181 tcgtagccga aagcgccggc catgccgcag cagctcgatt cgacgctttc cacctgcagg
     3241 ccggggatca gctggagcac acgctcgacc gccggcatgg cggcgaaagc cttctggtgg
     3301 cagtgcccgt gcagcagcgc tcttccatcg gggatgggtt gcagcggcag ccgcagccgc
     3361 cccgcatcca gctcgccggc caggaattct tcgaacaggt ccgcgcgctc cgccaccacg
     3421 cccacctctt cgtcatccaa accgagcacc aggtactcgt cgcgcagcgt gagcaggcaa
     3481 gacggttcca ggcccaccac cggcacgccg cgctcggcat gcggtttcaa cgcttcgatc
     3541 acccgccgcg cctcgcgacg cgcttcgtcc accatgccca gcgcgaggaa ggtgcgcccg
     3601 cagcacagcg gccgcccgcc gtccgcgggc cgggcgatct cgacgcgata gccgccggct
     3661 gccagcaccc gcaccgccgc acgcagattg tccggctcga agtagttgtt gaaggtgtcg
     3721 gcgaacagca ccacggggcg gtcaagctgt tcgctcgcgg gagaggagga aactccctct
     3781 cccgcagacc aagaggtccc tctcccctcg cgggagaggg tagggtgagg cggcgacagc
     3841 gcagaagaaa ctccgagaaa cgtgtcttgc cgccaccgcg gcagcgcgcg ccgcgcgctc
     3901 aaccccagcc acagctcgga caggcgcgcc aaggccggca cgcggtcgcg caggttgacc
     3961 agtggtgcca gccgcgccgc ccacggcgcg tagcgcggca gataagcgat cagcttgtcg
     4021 cgcaacccgt agccgcggcg ccggtgatag tggtgcagga actccacctt catgcgcgcc
     4081 atgtccaccc cggtggggca ctcgcgcttg cagcccttgc agccgacgca caggtcgagc
     4141 gtcgcgtaca tctcgtccga ggtgagcgcc tcgccgccga gctggcccga cagcgccagc
     4201 cgcaaactgt tggcgcgccc gcgcgtcaga tgctgctcct cgagcgtggt gcggtaggac
     4261 gggcacatgg tgcccgcgtc gaacttgcgg cagtggccgt tgttgttgca catctcgacg
     4321 gcgccggcga aaccgcccca ctcgctccag tcgagcgcgg tgtctagcgg acgcgtcgcg
     4381 tacccgggct tgaaccggaa cagctcgcgg tcgtccatct tcttcggctg cacgatcttg
     4441 cccgggttca tcaggccgcg cgggtcgaac agcgccttga cctcggccag cgccgcggtg
     4501 aggcgcgcgc ccaggatcgg ctcgatccac tccgagcgca ccagcccgtc gccgtgctcg
     4561 cccgagtagg cgcccttgaa gcgctgcacc agcgcgcagg cttcctccgc gatggcgcgc
     4621 atcttggccg cgccgtcctc cttcatgttg agcaccggcc gcacgtgcag cgtgcccacc
     4681 gacgcgtgcg cgtaccaggt gccttgcgtg ccgtggcgct cgaagatctc gttgatgcga
     4741 tgcgtgtact cggccaggtg ctcgagcggc acggcgcagt cctcgatgaa ggacaccggc
     4801 ttgccgtcgc ccttcatcga catcatgatg ttgaggccgg ccttgcgcac ttcccagatg
     4861 tccttctgca gcttcgcgtc ggtcacttcc acgacgctgc ccggcagacc gagatcggcc
     4921 atcagctcgg acaggcgctt cagccgcgcg atctgcgcat cgcgctcctc gcccgcaaac
     4981 tccaccagca gcactgcgcc gggcgcgccg cggacgaact gccgcagcac gtcgcggtac
     5041 tgcgggatct cgtgggccag gtcgagcatc gtccggtcca ccagctcgac ggcatcgggc
     5101 tcgagcggca cgatgtggcg cgccaggtcc atcgcctggt ggaaggtcgg gaagtgacac
     5161 gcgccgagcg ccttgtggcg cgggatcggc gagagcttga ggcgcagccg gcggaagcag
     5221 gcgagcgtgc cctccgatcc gaccaacaga tgggccaggt tgtgcggcag gtgcggctgc
     5281 accatgtcca ggttgtaccc ctgcacccgg cgcagcaccg tgggccagcg cgcggcgatc
     5341 tcctccgctt cgcgccgcac gatggcgtgc accttctcga tcaaggcccg gtagccgggc
     5401 gggccgctcg gcagctccgc ctccggcccg aaccagtagc actcgccgga cggcagccag
     5461 gcctcgatgc cgagcacgtt gtgcaccatg ttgccgtagc ggatcgagcg cgagccgcag
     5521 gagttgttgc cggccatgcc gccgagcgtc gcctgcgccg aggtggagac gtccaccgga
     5581 taccacaagc cgtgccggcg caggtcggcg ttgagccgat cgagcaccac gccgggctgc
     5641 acttcggctt cgcgccgctc ggcatcgacc gcggcgacgg cgttcaggtg cttgctgtaa
     5701 tcgatcacca gcgcggcgcc gacggtctgc ccgcactgtg aggtgcccgc cccgcgcggc
     5761 aacagcggaa cgccggcctc gatcgcgatc tcggctgccg cgcgtgccgc ctcctcggtg
     5821 cgcggcacca ccacgccgat cggctcgatc tgatagatcg aggcgtcggt gctgtagcgg
     5881 ccgcgggtga aggcgtcgaa caggacctcg ccgtccactt cgcgccgcaa gcgcgcttcg
     5941 agcggatgcg ccggcgccac atcgcgccgc acgaactgca ctggttgggc cacgctggtc
     6001 atgattgccc gtcgaattgt acgccctggg cggtcggcga ccagtcggcg catttccggc
     6061 ggatgcgcta agctccccac cgccatgcca cgcccgacac gcgccctctg gtttctgctg
     6121 tgctgcctgc ctgcatcggc gctcgccatc gacaacgcat gggtgcgcgt gtcgcgtgac
     6181 gccgcgccct gcgcggtggc gaccgcgccg gactgcggct atcgcgtgct ggtggcgctc
     6241 gccgagttgg acttgcgtgc gggcgacgcg tccaagcgct tggtgcgcgg ggacatcgcg
     6301 gtgttcggtc ccggccagga ctacaccgca ccttccggct cgtatttcga ggtcgcgatc
     6361 aaacccggcc atccgccggc gctggcgccc gccgagatca tcgccgccga gaagaacgca
     6421 ccgctctacg acggcgagga tttcttcgtg ttcgaggagc ggctcgcccc gggcgacacg
     6481 cgcgcgcggc acagccacag ccagcgcgtg gtgatccagc tcaacaaggc acgcctgcag
     6541 cagtggcccg acggcgcacc cgagctgttc gtcgaaaccg tgcccgagcg tccgaacttc
     6601 agcccgccgg tgatccacgt ggtgaagaac gtcggcgagg tgccgctgcg cggcatcgtc
     6661 atcgagttca agccggggcg ttgaaccgat gaaccggcgc agcgagatcc cgtccggcgc
     6721 ggccctgcac accggccgcc acttcctgca gatccccggt ccgaccaacg tgccggaccg
     6781 cgtgctgcgc gccatcgacc agccgaccat cgatcaccgc ggcccggagt tcgccgagct
     6841 cggcaaggaa gtgctggcgg ggatgaagca ggtcttcaag accgcgcatc cggtggtcat
     6901 ctaccccgcg tcgggcaccg gggcctggga ggcggcgctg gtcaacacgc tgtccccggg
     6961 ggatcacgtg ctgatggcgg agaccgggca cttcgccgcg ctgtggcgcc gcatggccga
     7021 gcggctcgga ctacaggtcg agttcctgcc gggagactgg cggcacggcg ccgactcggc
     7081 cgcgatcgaa gcgcgcttgg tgcaggacgg cgtgcaccgc atcaaggccg tggcggtggt
     7141 gcacaacgaa acgtccaccg gttgcgtgac gcgcgtgccc gaagtgcgcc gcgcgctcga
     7201 ccacgcggcc catccggcgc tgctgatggt ggacacgatc tcgtcgctcg cttctatcga
     7261 ctaccgcatg gacgaatggg gtgtggacgt cacggtcggc ggctcgcaga agggcctgat
     7321 gctgccgccg ggactgtcgt tcaacgcggt ggggccgaag gcgctggccg ccgcgaagcg
     7381 cgccacgcta ccgcgctcgt actggagctg ggacgaaatg ctcgccgcca acgccggcgg
     7441 tttctttccc tacacgccgg cgaccaatct gctgttcggc ctgcgcgagg cgttgcgcat
     7501 gctgcgggag gaagggctgg agaacgtgtt cgcgcgccac cagcggcacg cggaagccac
     7561 gcgccgcgcg gttgccggat ggggcctgga ggtgctgtgc gccgatccgc gcgagtacag
     7621 cgcatcgctc accgcggtgc tgatgcccga aggcgtggat gccgaccggg tgcgccagat
     7681 cgtcctgcag cgcttcgaca tgtccctcgg caccgggctg ggcaagctgc agggcaagat
     7741 cttccgcatc ggccatctcg gccacttcaa cgacctgatg ctggccggca cgctgtgcgg
     7801 cgtggagatg ggcctgcagc tcgccggcgt gccgctgcgc ggcagcggcg tcaaagcggc
     7861 gctggagcac ctcgcgcagg cgtaaacgcc gcttcgcgca ggcgccggat atcctcgcgc
     7921 cagccggcga cgagagggtc gcgctccggg tcctggccct ggtcccgcac ccggcgcttg
     7981 cggaacgcca gctcgtgctg cgcgttgacc agcgccgacg acgcacgcgg gcgcatgcgg
     8041 tcgatgcggc gtgtgcgcag cgcgcgatcg cgcacgatct cgccgtactc ggccggggtc
     8101 accgcaagac cgatttcgtt cgccagctcc tcgcggatca cccgccgttc ccacacaccg
     8161 cttcgccaca gcacgagcgc catgatcgcg acgaacggca ggaagatcgt cagctgggtc
     8221 agggtgccgg ccaggaaagc gtcgagaaac gccatgtcga gcgcaggccc cgccggcggc
     8281 tcgcccgagg cggcgcccgc gagcgctgcg aacaacggca gtgcattgtt gagcatgtgc
     8341 gtcgccaccg cgaggccgag cccgaggatc ggcgcgagga tgcgcagcca ccggcgccgc
     8401 gtctgcagcg ccagtccgag gcaggcgccg aagatcccgg tgaacagcgc gtgcccgccg
     8461 aacccgaaca gcgcgtaccg aaggccgagc tgccaaccgt acgacaccgc accctgctcg
     8521 acgtagctct tggccacgta cagcgccgct tcgagccagg tgaaccccag cccgaccagc
     8581 gcaccgtaca ccaggccgtc gcgcatgttg tcgaactcgg cgcgcagcaa ccagaacagc
     8641 aacgccagcc cgagcgcctt ggtggtctcc tcggcgatcg gcgccgagat cggcgcggcg
     8701 agcatcatcg ctgcatccgt gccgaggact tccgtgatgg ccggatgctg tgcgacccac
     8761 gcatcgacgg cgatgaagaa tgcggtgttc aggggcatgg cgagcgcggt ggcgatgcat
     8821 ccgccccaga gaaaggccgc ggcgaacaac cacggcgttt cgcgctcgcg ccgatccagg
     8881 aaccagagca cggccagcgg caccagcgcc agcaggaaag acagcccgag cgcccggtag
     8941 tagatcatcg cgacctccgg gcgcatggcg gccaggaggg tcagatggat gagcgtcgaa
     9001 acggccagca gaacgagcag ggtcgcaccg accagcacgg ctacactgcg ccgtcgcagc
     9061 ggcgagccca tcaacagccg atcgtacgcc gggtgcttcc gcctctccac aagcgtctcc
     9121 ctgcacgaga agcgcgcaga cccccgcaac cgcgccgggc ttttactcgg cggcgacgcc
     9181 cgccgcccag cgaatgccgc ccagcaagtg ttccaggaac aagggctcgc tgtaggtctc
     9241 gatcgtgtgc ccgagcgcgg tgtaccagct tctcccgccg tcgtattcct ggcaccaggc
     9301 catcgggtgg tgcgcgcgca tcgtcccgcc gctgtagctc ctctcgtcga tcaccagcaa
     9361 ggcgcgcccg tgcgcagccg ggtcctcgcg gaagttgtac cactcgtcgg tgcgcttcca
     9421 ctcgacgggc aagtgcaggg tcgaagggtg ggtcgcatcc accactttca gggtcgccgg
     9481 ttgaatgcga ggatggctct tgaagtaggc gcccaccaga ccgccgtacc aggcccagtc
     9541 gtattcggta tccgaggcgg agtgcacgcc gacgaatccg ccagcgcggc ggatgaagcg
     9601 ctcgaacgcc gcctgctggt cggcatcgag cacatccccg gtcgtgttca ggaagatcac
     9661 gacccgatag cggcgcaggt tttcgtcggt gaacgccacg gcggcctcgg tggcgtccac
     9721 gcgaaagccg tgcttctcgc ccagcccgtt gaccgccgcg acgcccgcgg cgatcgagtc
     9781 gtgccgatat ccggctgtcc tgctgaatac caggacgccg aatccggatg cggtcggctg
     9841 cgtggattgg gcggtgtgtg aagtgagtcc catggaaact ccgatgatgg cagccgcgac
     9901 ggtggacagg atgctgccgc ccggcaggtc agagggctgc gtggtggaca tggcccaact
     9961 cccaggaaaa ctctcacgat cttagcaggc ctgccgtata tcattcgcgg tatcgtgctc
    10021 cgcgccaagc cgggttagaa cggcgcatct gtcgcaaccg gaaggggata gccgatgggc
    10081 gacgacacgc acggcaagtc cgtcctacaa ctgcagactc gcggcgggtt cctggcgagg
    10141 gccgtcttgc tcgcgttctt ttccttcctg acggtgggcc tggggaacat catcatcgtc
    10201 ggcgtggatc cggccaggga gacggtcgag gcgttcaacc tcgggaaggg gttgtcgcaa
    10261 ctgctgtgct tctgggtggc cctgttttac tgggcgctgt gcgcgtggta cacggcgcgg
    10321 ctgctgctgg agaaatcctt cgcgcccttc gacccgcttc tgccgtgcgt gagcgagcgc
    10381 ttcgcacgcg gcctgatcaa atgggccccg cgcggcttgg gcgttgcggc gatcgttccg
    10441 atcgcattgg tgttcctcat cgcgcaagga atgaagcaca cccccctgtg ggtgcgaatc
    10501 tcgcccattg gcatcgccgc ggtgttcttc ggcttcgtcg tgtttcgccg gaagttcttc
    10561 ggcgacctgg tcgagctgct ccctcaggag cccggctacg gctaccgccg cttcagccgc
    10621 atcaactgga aaggttggct gctgatctcg gtgctcggcg cgatctcgtt ctcgaccctt
    10681 ctgttgctgt ggatcgacgg tgtcgcggtc gcacgctggg ttggtgcgcc ggcgcttgtg
    10741 ttgatcgcgc tcgggtcatg gagcatcttc ggcgggttcg tgctgatcta tctgccgatg
    10801 tccctgaaat ggccggcatt gacgctctgg ccgttcgtgc tggcggcact gttctcgttg
    10861 tggatggaga accacaacgt gcccgtcggg ccgccgccca aagcgggagc cgcgccagcc
    10921 gaatgggtgc ggcccacgct cgacgagcac tggcggcaat ggcgcgccgc gctcgacgcc
    10981 acgccatgca aagacaaggc gatctatctc gtttccatcg cgggaggcgc gacacgcgcg
    11041 gcgttctggg gcgcacatac gctggcagcg ctcgagcacc acgaacgcac gctggcggcc
    11101 gcagagaaac gcgcgccgtg cttcgcgcgc agcacgttcg ccatcagcgg cgtctccggc
    11161 ggggcgttgg gagcggccac tttcgtctcc ctgctcgcgc aagagcaggc cgtgcagcac
    11221 caatggggcg aattgttgcc cttggcggca gccgacttcc tcggccgcga cgtgttggcg
    11281 ccggtgctgg gatacatgct gttccaggac gggcttcagc ggttcctgcc ttatcccttc
    11341 gcatccctgg atcgctcgca cggcctggag gacgcctgga tatcggattg ggccgacatg
    11401 gtgcaggagc tttctccaga ggaaatgcat cgcggcggcg cgccgaactg gtttgcgcgg
    11461 ccgatgcgcg acctgtacgc ggacggccgc ggcacgacgt tgcccagcct gttcctgaat
    11521 accacccgcg tcgcagacgg caagaaggtc gtgcagtcca acctgcgctt cgtcccgaac
    11581 gatgcatacg acctctacgc gcgggggatg ctgaccggcg aactgacgct tgcgggcgcg
    11641 gtgcacaaca gcgcgcgctt cacctacgtc agtccggcgg gccgcgcatg gcgctgggac
    11701 gagatgcgcg gcagatcgac gccctggggc tatgtcgtgg acggcggcta cttcgagaac
    11761 tcgggctcgg cgaccttggc gccgctgatc cgcgagatcg gacggctcga tccggacaat
    11821 gcccgcaggc tcgtcctgat catgctatcc aacgacccct ccgatggggc gacggattac
    11881 gtgtgcgaag atccggcgcc acaactcgag ccgaagcgcg cgggcccccg cgaaagctcg
    11941 ccgcggttcc tgcgcgaacg actgcgaaag ccgggcatat ttgccatgga ggttgcggct
    12001 ccggcgatcg ctttgtacca gacgcgggca tcccgggcac atgcggcgga cctgcaagcg
    12061 aagaatgcgg tggacaggga atgcgcgagc ggccggatcg tggagctgcg cattcccatc
    12121 ccggcgagcg caaccgatga tccgccgatg agctggttcc tgaacgagcc caccatccag
    12181 ggactgacgc aacggataac cgagtctgcg gcagccgccg atacgccggc gcgccgcctg
    12241 tgcgagaacg tggagctgtt gaagtggcgc atccagaccc cggtcgcgga cacctcaccg
    12301 cgcctgctgc tcgagcactg ccggcagcga ccgcccgccg agtagctcag gcgacggcca
    12361 acactgcttc ggcgaactcc ttcggcgctt cctgcggaag gttgtgaccc accagcggga
    12421 tcacgcggcg ttcgtatcgc ccggtgaaaa accggccgtg tttctcggag ccgccttcgg
    12481 gcatgacgcc gtcgccacct ccgtccagcg cgatgctggg cacggcgatg cgcggctgca
    12541 tcgccagccg gcgctcggtg tcttcgacgt tcggatctcc cggcaccagc ccgaagcggt
    12601 ggcggtacga gtgaatcacg acgggcacga aatcgggatt gtcgaaggac gcggcgctgc
    12661 gctcgaaggt cacgtcgccg aaacgccagt tgggagacca cagctgccac agcagcttgc
    12721 acagcgcacg gcggttctgc tcgagaccgg cgcggccgcg ttcgccgtgg aagtagtact
    12781 ggtaccagta gcgcagctcg ttctccggcg ccgccggccg gccggaggcg gtgatgtctt
    12841 gaatgttgta accaccgccc gataccagcc cgcgcacgcg ctccggccac agcgccgcga
    12901 cgatgcaggc ggcgcgcccg ccccagtcgt acccggccag caccgcgcgc gagatcgcca
    12961 gcgcatccat caagacgagc aggtcgtggg ccagcaccgc ctgctgcccg gagcgcagcg
    13021 tatccggcga ccggaagcgc gtcggtccgt agccgcgcag gtagggggtg atcactcgac
    13081 atccggccga gacgaggatg ggcgtgacct cgtcataggc gtgcacgtcg taggggaagc
    13141 cgtgcagcaa taggatcggc gcaccggtcg cgctgccgtc ttcttcgtaa gcaacactga
    13201 ggtcgcctgc gtcagcgcgc ttcaacatga cagggcatgg tcaggcgctg gcgaggccct
    13261 tcagatagcg caggtcctcg tccagacgcc ggtcgatgtc gcgaaatacc tcggcgtgct
    13321 gcgtgagatc ctcgcccacg aaccccttcg atttcatcgg caccagatgg aagttctcga
    13381 aggagacgta gccgtcgtac ccgacccgtt tgagcaggcg catcaattcg gcccaatcca
    13441 cttggccgtc ggccattgaa gcgaagaccc agcgccacgc gcccttctcg cgtacccagg
    13501 cgacgttctt cgcgtgcacg tagtcgagat acggaccgag cagttccaga cccatctgcc
    13561 aggactcccg gccctcgatg atgagattgg cggggtcgta gttgacgccg atcctcgcag
    13621 gatcgaagtg ctggaccagg cgataagcca ggccggcgct cggtgcgatg gtgccgaagt
    13681 gggtctcgat gaccggtttg acggcgaagg ccgcgatcgc ttcgatcgcc ttgccgagcg
    13741 catcgacggt ggcctgatac agctcgccgt agttgcgcga gccgtcgtag atcaccgcgc
    13801 cgggccggta ccgcgggcag cccatgatgg cgcacgcttc cgcgaaccag cgctgctggg
    13861 cgacatcgcc gatcggtgcg tagccggtga ggcacgacac ctccaggcca tgagcgtcca
    13921 ccaggcgctt gatgtcgcgg gcgcgagcaa ccagctcctt gggcgcaacg tggtagtcct
    13981 cgtggacgcg ccactccacg ccgtcgtagc cgtgcgcgcg cagcttctcc acgacctgct
    14041 cccgggtcaa ttccggcagc acggcggttt gtacgctgaa tttcatgacc gggctattct
    14101 actgacattc ccgtaaatgc ctaatgccaa ctcgacacgt gggttgaacc accccgcccg
    14161 cttcgcgggc acctctcctt atcaaggagg ggaattctct tcctcccgac gcggtatcat
    14221 tgaccggacc cggaatctcg acaggaaaag ccatgagcaa gcctcgtcat ttcgagccca
    14281 agggcgtcat ccccgcctgc ctgctgccgt tcaaggaaga cctgagcatc gacgcgcgcg
    14341 cgttccgcaa gcacctgcgc cacgtcgcag cggtacccgg cttgtccgcg ctcaccatca
    14401 atgcgcactc gaccgaggtg tcctcgtgca gcttcgagga gcagcaggtc gtgctggaga
    14461 tcgcgctcga cgagcttggc gaccgcatcc cgctggtgaa cggcatctac gcggacggaa
    14521 gcctggaggc agcgcgcctc gcccgcatgg cggcgcaggc cggcgcgtcc tgcctgctgg
    14581 tgttcccgcc gaatggcctg ggcctgggcg cgcgcgaacg gccggagatg gtcatcgctc
    14641 acttcaaagc catcgccgac gcgaccgatc tgccgctgat cgccttcgag tatcggctgg
    14701 agtcggggct gggctacccg ctggatacgc tgttgcagct ggccgaccag gtgccgacgg
    14761 tccgcgcgat caaggactgg tgcaacaacg tcccgctgca cgagcgccac atccgggtgc
    14821 tgcagaacct gccgcgaccg gtcaacgtgc tgaccacgca cagcgcctgg ctgctgagct
    14881 ccctggtgct gggatgcaag ggcctgctgt ccggggcggg aagcgtgatc gcggatctgc
    14941 aggcggagct gttccaggcg gtgcagggca acggcctcgc gcgggcgcgt gagatcaacg
    15001 accgcatctt tccgctgacc gaggtgttct acgccgaccc cttcgtggac atgcacaacc
    15061 gcatgaagga ggcgctggtg atcctggggc gcatcccctg cgccgcggtg cgtccaccgc
    15121 tgatcaagct gagcgaggcc gaggtcgcgc gcatccgggg cgcgctgcag caggcgcgcc
    15181 tgcttcccga gagaaagcgc accgccgccg ccgcgtgaga tcgcgccggg cggatctgcc
    15241 gctctgcacg aagatcaggt tccgtcggag gcgtcgctgc tcaccgggat ccgatccgcc
    15301 gcgccgctgc cccgggccgc gcgcgtgagg cggtccctga ccgcggccca atccgcggtc
    15361 tcgggcggtg cctccaccag gatggccaca cagcccgatc ggtcgagctg gcgcagcccg
    15421 gcgtagaggt gctgcgcata gctcgcggca tccagcgggg cgtgcatcca caccgtggcc
    15481 gcagacgagg tcgggcgcga atgccgcgcc agcacggcga ccggtccgtg cgccgcctgc
    15541 tcgcggacgg tcgcatccag cattggccgc ggcaccaggc gcagcggcag ctgcggtgcg
    15601 tagtgggccg caaggccgcc gggcgcgcgc ggcgaagctg caccgccgcg cgtcaccggc
    15661 gcgtgcagca aggcgccaat ctcctccgcc gtgatgtggc ccggtcgcag cagcaccggc
    15721 gcgccggtcg acagatccag gatggtcgat tcgatcccga cggggcacgc gccgccgtcg
    15781 aggatcaggt ccacgcgctc gcccagctct tcgcgcacgt gttgtgccgt ggtcgcgctc
    15841 acccgcccga aacggttcgc ggagggcgct gcgatgccgc cgccgaaggc ctgcagcagc
    15901 gcctgggcga ccgggtgcga cggcacgcgc aggccgaccg tatcctgccc gccggtgacg
    15961 acgtcgagca ccttcggggc gcgcttcagc accaaagtga gcgggccggg ccagaagcgc
    16021 tcggcaagct gccgcgccgc gtccgggatt tcgcgcgccc agcgctccag atcgccgcta
    16081 cccttcagat gcacgatcag cggatgggtg gccgggcgcc ctttcgcctc gaagatgcgc
    16141 ttgacggcct tgcgcttcga ggcgtccgcg cccagcccgt acacggtctc ggtc
//
LOCUS       DASPGE010000141         3191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1446154, whole genome shotgun sequence.
ACCESSION   DASPGE010000141 DASPGE010000000
VERSION     DASPGE010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446154"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1607
                     /locus_tag="VD791_04435"
     CDS             <1..1607
                     /locus_tag="VD791_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXV07250.1"
                     /translation="MGYSLDLLDRPIVGIAQTGSGFNNCHRTMPELVEAVKRGVLAGG
                     ALPLEFPTVSLGEIFLSPTSLMFRNLMSMDTEEMIRGQPMDAVVLVGGCDKTVPAQLM
                     GAASADLPAVQLVTGPMMAMPWHGERLGACTDCRRFWARHRAGDIDAAGIAEVEGNLA
                     TTAGTCAVMGTASTMACIAEALGMALRDSAAIPAVHADRLRMAEASGRRAAEMAIAGG
                     PRPSEIITPRSVENALRVLLAVGGSTNAIIHLTAVAGRRGIAVDLKRLNRLSDETPVL
                     LDLKPTGQHYLQDLHAAGGLNALLWELRDLLHLDCCTVEGGTLADRLSAPPRWVDHAV
                     VRRRSEPIDKAGGLVALFGNLAPGGAILKRSAADAALFEREGRAVVFESLEDLAGRID
                     DPALDVAADDFLVLKNAGPCSASGMPEAGYLPIPKKLAQAGVKDMVRISDARMSGTAY
                     GTVVLHVTPDAASGGALALVRSGDRIGLSVRDRRLELLVPEEELGRRREGLRVPQRPA
                     RGYARLYAEHVLQADRGCDFDFLAGG"
     gene            complement(1837..>3191)
                     /locus_tag="VD791_04440"
     CDS             complement(1837..>3191)
                     /locus_tag="VD791_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HXV07251.1"
                     /translation="DDLGWKDVGYHGSSIRTPTLDRLAGGGARLEHFYVQPYSTQTRA
                     AVLTGRYPMRYGLQTMQIQWFSEFALPADERTLAQALGDAGYRTVLIGKWHLGHAQKS
                     AWPGRRGYDHFYGHLTGQIDYLKKTDRGGRPDWWRGDKAIKEEGHATALLAREALAIV
                     SRHDKSKPLFLHLAFAAPQAPHQGAKPFIDYYHADDQQLRAYRAMISGVDSALKEVLD
                     ALRKRSMEEGTLVVFHGTTGGAVRREHPIGDGDTEVNVADNGPYRDGRGSLYEGALRA
                     VALVSWPGRVQPGIVTELIHAVDLYPTLIKQGGGTLDQRKPVDGMDLWPTIAQGEPSP
                     RREALLNVEEFRGAIRVGDWKLIVQATLPSSVQLYNLRADPSEEDNQAERDGERVRTM
                     LRRLTEFAWDMAPAKYLEELAKPRRVEAPIHWRDNPARPDSGGQEGRGGTARQIPQQR
                     "
BASE COUNT          434 a         1095 c         1141 g          521 t
ORIGIN      
        1 ccatgggcta ttcgctggac ctgctcgacc ggccgatcgt cggcatcgcg cagaccggca
       61 gcggcttcaa caactgtcac cgcaccatgc ccgagctggt ggaggcggtg aagcgcggcg
      121 tgctcgctgg cggcgcgctg ccgctcgagt ttcccaccgt gtcgctgggc gagatctttc
      181 tctcgccaac cagcctgatg ttccgcaacc tgatgtcgat ggacaccgag gagatgatcc
      241 gggggcagcc gatggacgcg gtggtgctgg tgggcggctg cgacaagacg gtgccggcgc
      301 agctcatggg cgccgcctcg gccgacctgc ccgcggtgca gctggtgacc gggccgatga
      361 tggcgatgcc ctggcacggc gagcgcctgg gcgcgtgcac cgactgtcgc cgcttctggg
      421 cgcgccatcg cgccggcgac atcgacgccg ccggcatcgc ggaagtcgag ggcaatctcg
      481 ccaccaccgc gggaacctgc gcggtgatgg gcaccgccag caccatggcc tgcatcgccg
      541 aggcgctggg catggctctg cgcgacagcg ccgcgattcc cgccgtgcac gccgaccgcc
      601 tgcgcatggc ggaggcgagc ggccgccgcg ccgccgagat ggcgatcgcc ggtggcccgc
      661 ggccgagcga gatcatcacg ccgcggtcgg tggaaaacgc gttgcgcgtg ctgctggccg
      721 tcgggggctc gaccaacgcg atcatccacc tgacggcggt ggccggccga cgcggcatcg
      781 cggtcgacct gaagcggctc aatcggctca gcgacgagac gccggtgctg ctggacctca
      841 agccgaccgg ccagcattac ttgcaggacc tgcacgcggc cggcggtttg aacgcactgc
      901 tgtgggagct gcgcgatctg ctgcatctgg actgctgcac ggtggaaggc ggcacgctgg
      961 cagaccggct gtcggcgccg ccgcgctggg tggatcacgc ggtcgtccgc cggcgcagcg
     1021 agccgatcga caaggccggc ggcctggtgg cgctgttcgg caacctcgcg cccggcggcg
     1081 cgatcctcaa gcgctcggcg gccgatgccg ctctgttcga gcgcgaaggc cgtgccgtgg
     1141 tgttcgagtc cctggaggat ctggccgggc gtatcgacga tccggcgctg gacgtcgctg
     1201 ccgacgactt tctagtgctg aagaacgccg gtccgtgcag cgcttcgggt atgcccgagg
     1261 ccggttacct gccgatcccg aagaagctgg cgcaggcggg cgtcaaggac atggtgcgca
     1321 tctccgacgc gcgcatgagc ggtaccgcct acggcacggt ggtgctgcac gtgacgcccg
     1381 atgccgccag cggcggcgcg ctggcgctgg tgcgctccgg cgatcgcatc ggcctgagcg
     1441 tgcgcgacag gcggctcgag ttgctggtgc cggaagagga gctggggcgg cgccgggagg
     1501 gacttcgcgt gccgcagcga ccggcgcgcg gttacgccag gctgtacgcg gagcacgtcc
     1561 tgcaggccga tcgcggctgc gatttcgatt ttctggcggg cgggtaacgg agcgtaacac
     1621 ccgatcgcgg ggcacccctc accgccaccc tccgccttct cccgcaaggg gcgcaagcgg
     1681 agagggggtt gctcgacgac gcaaacgggc gcggccctcg gggccgaggg ctcagttcct
     1741 gcccttctgt cgcaggatga tcaggacgac gaggatcact acccccaccg ccatcgcgat
     1801 atcgagcatg gacatgggtt gcctccttga tgcgcgctag cgttgttgtg gtatttgccg
     1861 agcggtcccg cctcggccct cctgtccgcc gctgtcgggg cgcgcggggt tgtcgcgcca
     1921 atggatcggc gcctccactc ttcttggttt tgccagttct tccaagtact tggcaggggc
     1981 catgtcccag gcgaactcgg tcaagcggcg aagcatagtt cgtacccgct caccgtcgcg
     2041 ctccgcctga ttatcctcct ccgaggggtc ggcgcgcaga ttgtagagct gcacgctgct
     2101 gggcagcgtg gcctgcacga tgagtttcca gtcgcccacc cggatcgcac cgcggaactc
     2161 ctcgacgttg agcagcgctt cgcggcgcgg cgagggttca ccctgggcga tcgtgggcca
     2221 caggtccatg ccgtcgacgg gcttgcgctg gtcgagcgtg ccaccgccct gcttgatcag
     2281 ggtgggatac aaatccaccg cgtggatcag ctcggtgacg atgcccggtt gcacccgccc
     2341 gggccacgac accagcgcca ccgcccgcag cgcgccttcg tacaggctgc cgcgcccgtc
     2401 gcggtagggc ccgttgtcgg cgacgttcac ctcggtgtcg ccgtcgccga tcgggtgctc
     2461 gcgcctcacc gcgcccccgg tcgtgccgtg aaacaccacc agcgtcccct cctccatgct
     2521 gcgcttgcgc agcgcatcca gcacctcctt gagcgcgctg tccacgccgc tgatcatcgc
     2581 acggtaggcg cgcagctgct ggtcgtcggc gtgatagtag tcgatgaacg gtttcgcgcc
     2641 ctggtgcggc gcctgcggcg cggcgaaggc gaggtgcagg aacagcggct tggacttgtc
     2701 gtgccgcgag acgatcgcga gcgcctcgcg cgccagcagc gcggtggcgt gcccctcctc
     2761 cttgatcgcc ttgtcgccgc gccaccagtc gggccggccg ccgcggtcgg tcttcttcag
     2821 gtaatcgatc tggccggtga ggtgtccgta gaagtggtcg tagccgcggc gcccgggcca
     2881 ggcgctcttc tgcgcgtggc ccaggtgcca cttgccgatc agcacggtgc ggtagcccgc
     2941 atcgcccagg gcctgcgcca gcgtgcgctc gtccgcgggc agcgcgaact cgctgaacca
     3001 ctggatctgc atggtctgca gcccatagcg catgggatag cggccggtga gcaccgcggc
     3061 gcgggtctgc gtggagtagg gctgcacgta gaagtgctcc agccgcgcgc cgccaccggc
     3121 gagccggtcg agggtggggg tgcggatgct gctgccgtgg tagcccacgt ctttccatcc
     3181 gagatcgtcc a
//
LOCUS       DASPGE010000142         4867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_744415, whole genome shotgun sequence.
ACCESSION   DASPGE010000142 DASPGE010000000
VERSION     DASPGE010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744415"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VD791_04445"
     CDS             complement(<1..587)
                     /locus_tag="VD791_04445"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652018.1"
                     /note="Catalyzes the reversible hydrolysis of the amide
                     bond within dihydroorotate. This metabolic intermediate is
                     required for the biosynthesis of pyrimidine nucleotides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HXV07252.1"
                     /translation="MRIHIKGGTLIDPAQGTQGAGDLFIADERIAGAGAAPPGFAADR
                     TIDAAGLMVCPGLVDLSARLREPGFEYKATLESEMQAAVAGGVTSLVCPPDTDPPLDE
                     PGLVEMLKFRARSLKQARVYPLGALTQGLKGERLTEMAELRDAGCIGYGQADAPIADR
                     QVLSRALQYATTFGFCVWLRAQDPSLAHGGVAHDG"
     gene            complement(587..1537)
                     /locus_tag="VD791_04450"
     CDS             complement(587..1537)
                     /locus_tag="VD791_04450"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HXV07253.1"
                     /translation="MSGNPQLSPEGKLRHLLTIEGLPPEILVQILDTARSFVSVTERE
                     VKKVPLLRGKAIFNLFFEPSTRTRTTFEIAAKRLSADVLNLNIATSSQTKGETLLDTV
                     ANLSAMHADMFIVRHSQSGAAHLIARNVGPHIHVVNAGDGRHAHPTQALLDMFTIREY
                     KGGFNHLRVAIIGDVLHSRVARSEIQALTTLGVPEVRVIGPRTLLPAHVEQLGVQVHH
                     DMSEGLRDVDVVIMLRLQSERMDGALLPSPQEFFKYYGLTPDKLAHARPDAIVMHPGP
                     MNRGIEIDSSVADGGQSVILPQVTFGIAVRMAVMSILAGN"
     gene            complement(1542..2042)
                     /gene="pyrR"
                     /locus_tag="VD791_04455"
     CDS             complement(1542..2042)
                     /gene="pyrR"
                     /locus_tag="VD791_04455"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HXV07254.1"
                     /translation="MKLPDPEALLDTLAAKIRADLRPETALVGIHTGGVWVAQRLHQM
                     LGVRLPMGTIDVAFYRDDYKKKGLATGVRPSDIPFPVEDAEIILVDDVLYTGRTIRAA
                     MNEIFDYGRPNCIRLAALVDRGERQLPICPQYVGAQIAATRDQTIELKRDPAGRLALA
                     LVARPG"
     gene            complement(2039..2494)
                     /gene="ruvX"
                     /locus_tag="VD791_04460"
     CDS             complement(2039..2494)
                     /gene="ruvX"
                     /locus_tag="VD791_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HXV07255.1"
                     /translation="MSGGEPARGTVLAFDFGEKRLGVAVGELELALAHPLVTITAESD
                     KERLAAVERLVSEWAPVLFVVGLPMHLDGSDHALAKRCRRFAQRLRGRFGCPAHLVDE
                     RLTSATAGIALAEAGVRGRRQKDVLDRVAAQQILQAYFAGGVTNPDQPA"
     gene            complement(2544..3104)
                     /locus_tag="VD791_04465"
     CDS             complement(2544..3104)
                     /locus_tag="VD791_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HXV07256.1"
                     /translation="MHSVNLTHHFLIAMPNMVDPNFARALTYICEHNEQGALGVVVNR
                     PIEMTLGALFEQIEIPLRDRTHAQLPVYFGGPVQVDRGFVLHRPAGTWQSTLSINREI
                     GLTTSKDILEAVGEGRGPAEILVSLGYAGWAPGQLEQELGQNAWLTVPADPDLIFNLP
                     AERRFAAAMDLLGVDLSHLSDDVGHA"
     gene            3280..3846
                     /locus_tag="VD791_04470"
     CDS             3280..3846
                     /locus_tag="VD791_04470"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV07257.1"
                     /translation="MATERELATFLAEVERRAFKQAVFAVRNDQVALDLVQDAMLRLV
                     EKYAGKPAAELPLLFQRILQNAIRDHYRRSRVRSLWTTLLSSLQGKDGEEDRDPLDTL
                     RTDAGANRLGGPAEQAERGQVLRLIEQGLEALPARQREAFLLRYWEQLDVAETAQAMG
                     CSEGSVKTHCSRATHSLAIFLKSRGVEL"
     gene            3843..4292
                     /locus_tag="VD791_04475"
     CDS             3843..4292
                     /locus_tag="VD791_04475"
                     /inference="COORDINATES: protein motif:HMM:NF023699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HXV07258.1"
                     /translation="MNEQELPRKVAQRLEEGLETISPAISRRLVTAREAALARVHGGE
                     ALVGRSANGTVGAALAGAPGRFLDRRILAPALGLVLVLLGVLYWQQAQRVHREYANAR
                     EYADARYADIGDLDADVLGEELPVTAYLDQGFEVWLYHHSPASERQQ"
     gene            4343..4705
                     /locus_tag="VD791_04480"
     CDS             4343..4705
                     /locus_tag="VD791_04480"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HXV07259.1"
                     /translation="MALAQDWPQLTLEQQRILAPLKDDWPNIEAPRRQKWLDIARRYP
                     TMPPQKQERLQARMRQWAAMTPEERTQVRERYRRLQEMPPEKREEIRRKWREYESLTQ
                     EEKDNLRQAHPAGRPAQQ"
BASE COUNT          766 a         1655 c         1700 g          746 t
ORIGIN      
        1 tggccgtcgt gcgccacgcc accgtgtgcg aggctgggat cctgagcgcg cagccatacg
       61 cagaagccga acgtggtcgc gtactgcagc gcgcgcgaaa gcacttgccg gtcggcgatc
      121 ggcgcgtccg cttgaccgta accgatgcag ccggcgtcgc gcagctcggc catctcggtc
      181 aggcgctcgc cctttagccc ctgggtcagc gcgcccaggg gatagacgcg cgcctgcttg
      241 aggctgcgcg cgcggaactt gagcatctcc accagcccgg gctcgtccag gggcgggtcg
      301 gtgtcgggcg ggcacaccag gctggtcacc ccgcccgcga ctgccgcctg catctcgctc
      361 tcgagcgtgg ccttgtactc gaagcccggc tcgcgcaggc gtgcggacag atcgaccagt
      421 cccggacaca ccatcagacc ggcggcgtcg atggtgcggt cggcggcgaa tcccggcggc
      481 gcggcgcccg cgcccgcgat acgctcgtcg gcgatgaaca gatcgcccgc gccctgggtg
      541 ccctgcgccg ggtcgatcag cgttccaccc ttgatgtgga tgcgcatcag ttgcccgcca
      601 ggatcgacat caccgccatg cgcaccgcga tgccgaaggt gacctggggc aggatcacgg
      661 actggccgcc gtcggccacg ctggagtcga tctcgatgcc gcggttcatc ggccccgggt
      721 gcatcacgat cgcgtccggt ctggcgtgcg ccagcttgtc cggcgtcagc ccgtagtact
      781 tgaagaactc ctgcgggctg ggcagcaacg cgccgtccat gcgctcgctc tgcaggcgca
      841 gcatgatcac cacgtccacg tcgcgcaagc cctcgctcat gtcgtggtgg acctgcacgc
      901 cgagctgctc gacgtgcgcg ggcagcaacg tgcgcggacc gatcacccgt acctcgggca
      961 cgcccagcgt ggtcagcgcc tggatctcgg agcgcgccac gcgcgagtgc agcacgtcgc
     1021 cgatgatggc cacgcgcagg tgattgaagc cgcccttgta ctcgcggatg gtgaacatgt
     1081 cgagcaacgc ctgagtcgga tgcgcgtggc ggccgtcgcc ggcgttcacc acgtggatgt
     1141 gcggtccgac gttgcgcgcg atgaggtgcg cggccccgct ctggctgtgg cgcacgatga
     1201 acatgtcggc gtgcatcgcc gacaggttgg ccaccgtgtc gagcagggtc tcgcccttgg
     1261 tctgcgagga ggtggcgatg ttcaggttca gcacgtcggc ggacaggcgc ttggccgcga
     1321 tctcgaaggt ggtgcgggta cgcgtgctgg gctcgaagaa caggttgaag atggccttgc
     1381 cgcgcagcag cggcactttc ttcacctcgc gctcggtgac cgacacgaaa gagcgcgcgg
     1441 tgtccaggat ctgcaccagg atctccgggg gcaggccttc gatggtgagc agatgccgga
     1501 gcttgccttc gggcgagagc tgcggattgc cgctcatcgg ctcaacccgg gcgcgcgacg
     1561 agcgccagcg cgagccgtcc ggccggatcg cgcttcagct cgatggtctg gtcgcgggtc
     1621 gcggcgatct gcgcccccac gtactgcgga cagatgggca gctggcgctc gccgcggtcc
     1681 accaaggcgg caaggcggat gcagttcggg cgcccgtagt cgaagatctc gttcatggcg
     1741 gcgcggatgg tgcgcccggt gtagagcacg tcgtccacca ggatgatctc ggcgtcctcc
     1801 accgggaacg gaatgtccga ggggcgcacg ccggtcgcca gccccttctt cttatagtcg
     1861 tcgcggtaga aggccacgtc gatggtcccc atcggcagcc gcacgcccag catctggtgc
     1921 agccgctgcg ccacccacac cccgccggtg tggatgccca cgagcgccgt ctccgggcgc
     1981 agatcggcgc ggatcttggc ggcgagcgtg tcgagcagcg cttccgggtc gggcagcttc
     2041 atgcgggctg atcggggttc gtcacgccac cggcgaagta ggcctgcaag atctgctgtg
     2101 ccgccacccg gtcgagcacg tccttctgcc gccggccacg cacgccggcc tcggcgagcg
     2161 caatgccggc ggtggcggag gtcagccgct catccaccag gtgcgccggg cagccgaagc
     2221 ggccgcgcag ccgctgtgcg aagcgcctgc agcgcttcgc caacgcgtga tcgctgccgt
     2281 cgagatgcat cggtaagccc accacgaaca ggaccggcgc ccattcgctc accaggcgct
     2341 cgacggccgc gagccgctcc ttgtccgact ccgccgtgat cgtgacgagc ggatgtgcga
     2401 gcgccagttc cagttccccg accgccacgc ccagccgctt ctcgccgaag tcgaaggcga
     2461 gcacggtgcc gcgggcgggt tctccgccgc tcatggccgg gcgcccgcgg cggggcgtcg
     2521 gctgcgtatc cgtgcggggg cgatcaagcg tggccaacgt cgtcggaaag gtgcgagaga
     2581 tcgacgccga gcaagtccat ggcggccgcg aaacgccgtt ccgcgggcag gttgaagatc
     2641 aggtcggggt cggcgggcac cgtcagccag gcgttctgcc ccagttcctg ctcgagctgg
     2701 cccggcgccc atccggcata gcccagggaa accaggatct cggccggccc gcggccctcg
     2761 ccgaccgctt ccaggatgtc cttggaggtg gtcagaccga tctcgcgatt gatcgacaag
     2821 gtggattgcc aggtgccggc gggacggtgc agcacgaagc cgcggtccac ttgcaccggg
     2881 ccgccgaaat acaccggcag ctgcgcgtgc gtgcggtcgc gcagcgggat ctcgatctgc
     2941 tcgaacaggg cgcccagggt catctcgatg ggccggttga ccaccacgcc gagcgccccc
     3001 tgctcgttgt gctcgcagat gtaggtgagc gcgcgcgcga agttcggatc caccatgttg
     3061 ggcatggcga tcaggaaatg atgggtgaga ttgaccgagt gcatgggcaa tgctcatttt
     3121 atcagcaggc ggcgtccgcc cggttaccgg caggcaaccg cgcgcaagcg cgggcttgcc
     3181 gggggccgat cggctgacac gcagcgaccg attttgctac catcgccgcg ccggccgtgg
     3241 acccaaaccc acgaagggcc ggcgcatccg agaacccccc tggctaccga acgagagctg
     3301 gcgacgttcc tggcggaggt ggagcgcaga gcgttcaagc aggcggtgtt cgccgtgcgc
     3361 aacgaccagg tcgccctgga tctggtgcag gacgcgatgc tgaggctggt cgaaaagtac
     3421 gccggcaagc cggctgcgga actgccgctg ctgtttcagc gcatccttca gaacgcgatc
     3481 cgcgatcact accgccgcag ccgcgtgcgc tcgctgtgga ccacgctgct gtcctcgctg
     3541 cagggcaagg acggggagga agaccgcgac ccgctggaca cgctcaggac cgacgccggc
     3601 gccaaccgcc tgggggggcc ggccgagcag gccgagcgcg gccaggtgct gcggctcatc
     3661 gagcaggggc tggaggccct gccggcgcgt caacgggagg cgttcttgct tcgttactgg
     3721 gaacaactcg acgtggcgga aaccgcccag gcgatgggct gctcggaggg cagtgtgaag
     3781 acccactgct ctcgggccac ccattccctg gcgattttcc tcaagtcgag gggagtggaa
     3841 ctgtgaacga gcaggagctg ccgcgcaaag ttgcgcaacg cctggaggaa ggcctggaga
     3901 cgatctcgcc ggccatctcc cggcgtttgg tcaccgcccg cgaggccgcc ctggcccgcg
     3961 tccacggcgg cgaagcgctg gtcggccggt ctgcgaacgg taccgtgggc gccgccctgg
     4021 ccggcgcgcc tggccgcttc ctcgaccggc gcatcctggc gcctgccctg ggccttgtcc
     4081 tggtgctgct cggcgtgctc tactggcagc aagcgcaacg cgtgcaccga gagtatgcca
     4141 atgctcggga gtacgccgat gcccggtacg ccgacatcgg cgacctcgac gccgatgtgc
     4201 tgggcgagga gttgccggtc accgcctacc tggaccaggg gttcgaagtt tggctctatc
     4261 accactcgcc cgcttccgag cggcagcaat agacctgctc ttcccgatcg ccctgaccgc
     4321 cgcgtcctgt gtcgtcgtgc ctgtggccct cgcgcaggac tggccacagc tgacactcga
     4381 acagcagcgc atcctcgcgc cgctcaagga cgactggccc aacatcgaag ccccacgccg
     4441 ccagaagtgg ctggacatcg cgcggcgcta tccgaccatg ccgccgcaaa agcaggagcg
     4501 gttgcaggcg cgcatgcgcc aatgggcggc gatgaccccg gaggagcgta cgcaggtgcg
     4561 cgagcgctac cgcaggctgc aggaaatgcc gccggagaag cgtgaggaga tccgccgcaa
     4621 gtggcgcgag tacgagagcc tgacgcaaga ggagaaggac aacctgcgcc aggcccatcc
     4681 ggccggccgg ccggcgcagc agtgagccaa ccgcaaagaa tttccaccgc ggaggacgcg
     4741 gaggacgcag aggaagcggc cacaggacat ggcgtcagcg gcctcatcga gggcaaatgg
     4801 cgctcctgct tgcacgatcc tttgaattcc tccgcgtcct ctgcgtcctc cgcggttcct
     4861 gttgttt
//
LOCUS       DASPGE010000143         2870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_861760, whole genome shotgun sequence.
ACCESSION   DASPGE010000143 DASPGE010000000
VERSION     DASPGE010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861760"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VD791_04485"
     CDS             complement(<1..624)
                     /locus_tag="VD791_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXV07260.1"
                     /translation="MLPAALLLVLFLTYPLGLGVWLGFTDAQIGRPGEWIGIENYVYA
                     VTDGVFRLSVWNTFLYTLIASVIKFGLGLWLALLLDEHIPFKALLRAVVLLPFIVPTV
                     LSAIAFWWIYDAQFSIVSWTLSKLGLIDGYIDFLGEPVLARGSVIAANVWRGVPFIAI
                     CLLAGLQTIPPSLYEAAAIDGATPWQQFWNVTLPMLSPIIAVVMTFSV"
     gene            complement(721..1785)
                     /gene="ugpC"
                     /locus_tag="VD791_04490"
     CDS             complement(721..1785)
                     /gene="ugpC"
                     /locus_tag="VD791_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HXV07261.1"
                     /translation="MASVELRRVRKAFGRHEVVHGVDVTIDDGEFVVLVGPSGCGKST
                     LLRMIAGLETITAGEISIGGKVVNEVEPADRDIAMVFQNYALYPHMTVFDNMAFSMKL
                     AGRGKDETRRRVDQAAKILGLTEYLDRYPRQLSGGQRQRVAMGRAIVRDPQVFLFDEP
                     LSNLDAKLRVQMRTEIKELHQRLKTTSVYVTHDQIEAMTMADKIVVMNGGNVEQIGSP
                     LALYDDPANLFVAGFIGSPAMNFLPGKLARDGARRLVTLADGNRLPVPGLALGDGHKV
                     TLGVRPEHLVIGDEGAPAEVVVVEPTGADTQVYCKLAEADLTVVSRERHAFRPGDTIR
                     LQPLPGKTYLFDAASGKRLA"
     gene            complement(1925..2686)
                     /locus_tag="VD791_04495"
     CDS             complement(1925..2686)
                     /locus_tag="VD791_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV07262.1"
                     /translation="MSLHRYRAPPDLLEGRVILVTGASAGIGRVAARTCARHGATVIL
                     HGRDTGRLEAVYDEIEAAGGPQAIVLPLDLAAATDRDFDNVAQAIRAQAKRLDGILHN
                     AAHSVPLGALQHERLDDWLRLLRVNVGAVFALTRACTPLLREAPDASVVLTGETHGLR
                     PAAYWGGFAVSKSALTAYNIIQAQEWEPCANLRINLVVPGRVDSPHRGRTHPGEAREE
                     RAGPESLMPLYLYLLGPDSAGVSGKVFEADDADPA"
     gene            complement(2709..2858)
                     /locus_tag="VD791_04500"
     CDS             complement(2709..2858)
                     /locus_tag="VD791_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXV07263.1"
                     /translation="MGVVRPAQRTPVPGLYLAGDYTANPYPATLEAAVRSGIACARMV
                     LAPVG"
BASE COUNT          467 a         1020 c          973 g          410 t
ORIGIN      
        1 caccgagaag gtcatcacca cggcaatgat cggcgagagc atcggcagcg tcacgttcca
       61 gaactgctgc cagggcgtgg caccgtcgat cgccgccgcc tcgtacaggg agggcgggat
      121 ggtctgcagc cccgccagca ggcagatggc gatgaacggc actccgcgcc agacgttggc
      181 ggcgatgacc gacccgcggg ccagcaccgg ttcgcccagg aagtcgatgt acccgtcgat
      241 cagaccgagc ttgctcaagg tccagctgac gatcgagaac tgcgcgtcgt agatccacca
      301 gaaggcgatc gccgagagca cggtgggcac gatgaagggc agcagcacga cggcgcgcag
      361 caaggctttg aacgggatgt gctcgtcgag cagcagggcg agccacaggc cgaggccgaa
      421 cttgatgacg ctggcgatca gcgtgtacag aaacgtgttc cagaccgaca ggcggaacac
      481 cccgtcggtc actgcgtaca cgtagttctc gatgccgatc cactcgcccg gccggccgat
      541 ctgggcatcg gtgaaaccca gccacacgcc caggcccagc gggtaggtca ggaacagcac
      601 cagcagcagc gccgccggca gcatgaacag cagccccagg aagccgcggt tgtcgaacag
      661 ccggctcagg ggcgactgca gcaaagccgg agcggatgcg gccggggcat gcatggacgg
      721 ttacgccagc cgcttgccgc ttgccgcgtc gaacagatag gtcttgcccg gcagcggctg
      781 caaacggatg gtgtcgccgg gccggaaggc gtggcgctcg cgcgacacca ccgtgagatc
      841 cgcctccgcg agcttgcagt acacctgcgt gtcggcgccc gtcggctcga cgacgaccac
      901 ctcggccggc gcgccctcgt cgccgatcac cagatgctcg gggcgcacgc ccagcgtcac
      961 cttgtgtccg tcgcccaggg ccagccccgg caccggcagc cggttgccgt ccgccagcgt
     1021 caccaggcgc cgcgcgccgt cgcgcgccag cttgccgggg agaaagttca tcgcgggcga
     1081 gccgatgaac ccggccacga acaggttcgc ggggtcgtcg tacagcgcca gcggcgagcc
     1141 gatctgctcg acgttgccgc cgttcatcac cacgatcttg tcggccatgg tcatggcctc
     1201 gatctggtcg tgggtcacgt acaccgaagt ggtcttcagc cgctggtgca gctccttgat
     1261 ctcggtgcgc atctgcacgc gcagcttggc atcgaggttg gacagcggct cgtcgaacag
     1321 gaacacctgg ggatcgcgca cgatcgcccg gcccatcgcc acgcgctggc gctggccgcc
     1381 ggaaagctgg cgcggatagc ggtccagata ctcggtgagg cccaggatct tcgccgcctg
     1441 gtccacgcgc cggcgtgtct cgtccttgcc ccgccccgcc agcttcatgc tgaaggccat
     1501 gttgtcgaac acggtcatgt gcgggtagag cgcgtagttc tggaacacca tggcgatgtc
     1561 gcggtcggcc ggctccacct cgttgacgac cttgccgccg atgctgatct cgcccgcggt
     1621 gatggtctcg agcccggcga tcatgcgcag cagcgtggac ttgccgcagc cggaaggacc
     1681 caccaggacg acgaactcgc cgtcgtcgat ggtgacgtcc acgccgtgga ccacctcgtg
     1741 gcggccgaac gccttgcgca cccgcctcag ctcgaccgag gccatcagcg ccgccgggcg
     1801 agccggcggc gggccggccg gatgtgcgcg cggcaaccgc gcggcagttc gagcaccacc
     1861 tctcgctccc ccgagtgatc gttgtgcggc agtggaggtc gaaccatacc acgccgccga
     1921 acggctacgc tggatccgcg tcgtccgcct cgaacacctt tccgctcacg cccgcgctgt
     1981 cggggcccag caggtagaga tacaacggca tgagcgactc cgggccggcg cgctcctccc
     2041 gcgcctcgcc cggatgcgtc ctgccgcggt gcggggagtc cacccggccg ggcaccacca
     2101 ggttgatgcg caggttggcg cacggctccc actcctgcgc ctggattatg ttgtaggccg
     2161 tgagcgcgct cttcgacacc gcgaagccgc cccagtaggc ggccggccgc aggccgtgcg
     2221 tttcaccggt cagcaccacc gaagcgtccg gcgcctcgcg cagcaacggt gtgcacgcac
     2281 gcgtgagcgc gaacaccgcc cccacgttga cgcgcagcag gcgcagccaa tcgtccaggc
     2341 gctcgtgctg caacgcgccc agcgggacgc tgtgcgcggc gttgtgcagg atgccgtcca
     2401 ggcgcttcgc ttgcgcgcgg atcgcctgcg ccacattgtc gaagtcgcga tccgtcgccg
     2461 ccgccagatc gagcggcagg acgatcgcct gcggtccccc cgcggcctcg atctcgtcgt
     2521 acaccgcctc cagccggccg gtgtcgcggc cgtgcaggat gaccgttgcc ccatggcggg
     2581 cgcaggtgcg cgcggccaca cgcccgatgc cggcgctcgc gccggtcacc aggatcacgc
     2641 ggccctcgag caggtcgggt ggcgctcgat agcggtgaag gctcatctgg ttgcgattcc
     2701 aggcatgctc acccgactgg ggcgagcacc atgcgcgcac aggcgatgcc gctgcgcacc
     2761 gcggcctcca gcgtggcggg ataagggttc gcggtgtaat cgccggccag gtacagaccc
     2821 ggcaccggcg tgcgctgcgc gggacgcacc acgcccaccg tgcaagcgaa
//
LOCUS       DASPGE010000144        20179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_627695, whole genome shotgun sequence.
ACCESSION   DASPGE010000144 DASPGE010000000
VERSION     DASPGE010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20179
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627695"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..898
                     /locus_tag="VD791_04505"
     CDS             35..898
                     /locus_tag="VD791_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07264.1"
                     /translation="MTVPALTPASSARRRFLVDAGALAAGALMAGAARAARINPPALL
                     DNQAGGYRVLPAGQVFCGGVIPNPGYEIVHALFNPWVPLDDAWGLIEAHLKSLGRPVQ
                     ALCGMELRIPQQLTVEGFRAFNAPYVEHLRKRDLLLGNYSAVCRTNVAPGRDAPKVPS
                     LHAFSYCLPGAHTGTTFCVSGTADIDARGKIVAEGDVSAAGMRARLQHCVDVITERLA
                     MLELDWSAATHIDLCVVEDIPGLMDAVVVPGLQGAASRGVRVHVARPPIIGAEVELEC
                     RGVRQELVLRA"
     gene            complement(941..2320)
                     /locus_tag="VD791_04510"
     CDS             complement(941..2320)
                     /locus_tag="VD791_04510"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HXV07265.1"
                     /translation="MRPAALTLGPVGLILAGALIAADGDAPQSLALAGGALTTAESGP
                     RAYEQLSLTLSQAEYRQIADGRTFYDTRWAFYWFEQGLWGRGPTSNADACATCHRANG
                     RGAPPREGDRDDTFVVQIGLPGKDANGGPRPDPNYGAQLQTQGVPRLIAPEARIAFDW
                     STHAERFADGETVELRAPRVRIEKLAYGPLGDDIMISARVAPPVIGMGLLDAVPEEVI
                     LALAARAAPQGISGRPNRVWDVEGKRFALGRFGLKANHPTLRQQVAAAFIEDIGLSTE
                     LFPEQNCPGAQQYCREMMVAGRPEITEYRLKAVESLLRFGAVPARRNPDDAGVRRGEM
                     LFAQAQCAACHVPELRTGEVPGQPALSQQVIRPYTDLLLHDMGEALADERPDFEASGR
                     EWRTPPLWGIGLTQTVNGNVNYLHDGRARTLVEAILWHGGEATASRDAFKAMPGTERE
                     ALLRFLESL"
     gene            2380..3738
                     /locus_tag="VD791_04515"
     CDS             2380..3738
                     /locus_tag="VD791_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HXV07266.1"
                     /translation="MFDDPLLLARTQFGLNIAFHILFPSITIALAWFLVYLRVRADRP
                     GAGWAQKAYELWVKIFALSFALGVVSGIVMSFQFGTNWPGFMQKAGNVAGPLLGYEVL
                     TAFFLEATFLGVMLFGRERVPRWLHTLSAVLVAVGTTGSAFWILALNSWMQTPQGFEV
                     IDGALHVVSWWDVIFNPSFPYRLTHMLLASGLTAAFLIAGVSAFQLLRNHAHQPAWRM
                     FRAGTAVGAVLIPIQIAVGDLHGLNTLEHQPAKIAAIEGIWTSGARVPLVLLAWPNPE
                     QRRNDYAVEIPGGASFILRHDWNAELEGLDRFADEHPPVRPVFFAFRVMVGTGVLMLL
                     LSWLASGYLLAKRRIPPWMLVAFALMTFSGWVATLAGWLVTEIGRQPYLVYGVITTAE
                     TASSVPAANIAITLIGYALVYAALLISYLVVLTQLARSAAGDAPVIPPSGELQPTGTV
                     RA"
     gene            3735..4730
                     /locus_tag="VD791_04520"
     CDS             3735..4730
                     /locus_tag="VD791_04520"
                     /EC_number="7.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006119 - oxidative phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HXV07267.1"
                     /translation="MSLDLPLIFAILMGLAILAYVILDGFDLGVGILLPGGQRPEQDV
                     MVSSIGPFWDANETWLVLGVGILLVAFPLAHGVVLSSLYLPVAAMLCGLVLRGVAFEL
                     RFKATGWHREVWNRLFFVGSLLASVAQGAMLAGVITGFASGPWFTLFGLLVGLGLACG
                     YALLGATWLVIKTEGELRSKALRWSRLALIGAAAGVAVISLATPLASPRILHKWFDWP
                     RIAFLAPLPLLTAAACFGIWSWVGRLQSGRTRLEWPPFVLAVWVFVFAFAGLAYSLFP
                     YLVIDRLTVWQAAAAEKSLWFVFIGACVVLPLIVAYTLLSYRLFWGKARTLTYGP"
     gene            4865..6946
                     /locus_tag="VD791_04525"
     CDS             4865..6946
                     /locus_tag="VD791_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HXV07268.1"
                     /translation="MDPLPPERAVEQAIAAHRHRPGALLPILHAVQDAIGYVPPSAIG
                     QIAHALNLSRAEVHGVISFYHDFRTSPPGRHVVRVCRAEACQSMDGVALEQHARARLG
                     TGWHQTTEDGAVTLEPVYCLGNCACSPAVMIDGKVYGRVTPARFDALLDAARGSAPSA
                     ARSPAERPEAMPAADDAVTVLVPRDTSAVSLGADAVADEIARLAAERRTPVRIVRNGS
                     RGVFWLEPLVEVQHAASGRVAYGPVQPEDVPGLFDAGFLHGGAHRLALGPTAEIDYLK
                     SQERLTFERCGVIDPLSLEDYAAHGGWQGLHHAVTMQPADIVEQVVQSGLRGRGGAAF
                     PAGIKWRTALNAPADRKYIVCNADEGDSGTFADRMLMEGDPYLLIEGMIIAGLAVGAT
                     AGYIYLRSEYPAAERVLEQAIDRARAAGYLGHDVIGGGKAFELEVRRGAGAYICGEET
                     SLLESLEGKPGQVRFKPPLPAIQGLFGKPTVVNNVVTLASVPIILERGAAFYRDFGTG
                     RSRGTLTIQLAGNIRRGGLVEKAFGVTLHELLYEYGGGSASGRPIRAVQVGGPLGAYL
                     PESQFDTPLDYEAFAALNAMIGHGGVVAFDDRVDMAHMARYAMEFCAVESCGKCTPCR
                     IGSTRGVEVVERIVRGERRPENHALLRDLCDTMLHGSLCALGGMAPYPVLSALNHFPE
                     DFGLAPGVKGA"
     gene            6984..9830
                     /gene="fdhF"
                     /locus_tag="VD791_04530"
     CDS             6984..9830
                     /gene="fdhF"
                     /locus_tag="VD791_04530"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HXV07269.1"
                     /translation="MYSIKELDYGTPRRVSGAPVTLEIDGKPVTVASGSSVMAAAVEA
                     GVRVPRLCATDMLEAFGSCRLCLVEIEGRKGYPASCTTLVEPGMKVLTQTPKLADLRR
                     GVMELYISDHPLDCLTCPANGNCELQEMAGVVGLREVRYGYDGANHLRAATDASNPYF
                     NFDPTKCIVCSRCVRACEDVQGTFALTVDGRGFASVISPGGSDFLASECVSCGACVQA
                     CPTATLMEKSIVERGQPEHSVITTCAYCGVGCSFRAEMKGGQVMRMVPDKDGHANHGH
                     SCVKGRFAWGYATHPDRILKPMIREKITDPWREVSWDEAIAHTAAEFQRIQREHGRGA
                     VGGITSSRCTNEETYLVQKLVRAAFRNNNVDTCARVCHSPTGYGLKQTLGESAGTQNF
                     DSVAHADVVMVIGANPTEGHPVFASQLKRRLREGAKLIVVDPRTIDLVRQPHVEAAYH
                     LQLRPGTNVAVINALAHTIFTERLWNEAFVRARCDLKEFEKWKAFIVQPYNSPEAMEP
                     ITGVPAADLRGAARLYATGGNGAIYYGLGVTEHSQGSTMVIGIANLAMLTGNLGRPGV
                     GVNPLRGQNNVQGSCDMGSFPHELPGYRHISDATTRALFEAAWGVTLESEPGLRIPNM
                     LDAALDGSFKGLYVQGEDIAQSDPDTQHVTAALSAMECLVVQDLFLNETAKFAHVFLP
                     GSSFLEKDGTFTNAERRVSRVRKVMAPLCGLADWEATVALSNALGYPMHYRHPSEIME
                     EIARLTPTFHGMSYAKLDRLGSIQWPCHERSPEGTPVMHVDEFVRGKGKFMLTAYVPT
                     DEHTSRMFPLLLTTGRVLSQYNVGAQTRRTANLVWHDEDKLEIHPHDAEQRGVREGDW
                     VGVRSRAGETVLRAHVTERIQPGVVYTTFHHPFSGANVVTTDISDWATNCPEYKVTAV
                     QVTRVSQPSEWQRHFTEIDHQQHELLEHRQRERA"
     gene            9847..10089
                     /locus_tag="VD791_04535"
     CDS             9847..10089
                     /locus_tag="VD791_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HXV07270.1"
                     /translation="MDPEKLIHMANRIADFFQAEPDRQAALEGIAGHLKRFWDPRMRR
                     EILRLVDEDRAQGLSPLVVDALHAHRKLIAPGELRA"
     gene            complement(10096..11508)
                     /locus_tag="VD791_04540"
     CDS             complement(10096..11508)
                     /locus_tag="VD791_04540"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HXV07271.1"
                     /translation="MAGFLACAICAPSANAHPIVSTDINRHVTLRVTDDRLEIRYLYE
                     MLEIAAINTARAWDADSDGSTSAAERDAYAASLGQELLRHLHVRLDDALVPLTLEGVR
                     WELGEGAMGLATWKLVVRFGARLPVTAATGTLDYRDDLRPEEVGWKEVMLTAGGSSGI
                     ARASVPSHDRSYELTDYAAISELPNPNETAAQALLRFAAAPVAETARAPDAPATLPVP
                     AAQAADHARGARAKPETAAPIRAAPAPVPRPESAERGPKPSADDSGRLQAHAQAPAPS
                     ASPPASAWRHYAWPFFKLGVHHIATGYDHLLFLLGLLLFRQSLGRLAAVVTAFTLAHS
                     VTLAVAAAGWITPPGALVELLIAASIAYVGAVTLARPHTPHGPWIALGFGLMHGFGFA
                     GALSAALSGVTDKGWLVALAGFNLGIEAFQLVLVVLAWPLLKRADAFAWSPWLRRLLS
                     AIVLAAGLAWTIARVGAVAG"
     gene            complement(11550..12284)
                     /gene="pncA"
                     /locus_tag="VD791_04545"
     CDS             complement(11550..12284)
                     /gene="pncA"
                     /locus_tag="VD791_04545"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HXV07272.1"
                     /translation="MSTLSRRNFIMAAGAVTASAALPLAGLRHALAAGAIKVGPSDAL
                     LVIDVQNCFIPGGTLPVPKGEEVVPLINELAGKFNCVVMTQDWHPAGHISFASTHGKE
                     PFSVIDMPYGKQVMWPDHCVQGTDDAKLHAQLDLTKANLILRKGFNPKVDGYSAFYDN
                     DKKSTTGLAGYMKARGVKRVFVCGLATDFCVMWSSVDARKDGFQVVVLEDASRGIDVR
                     GSMEAGWKEMEKAGVVRAMSTDLQFG"
     gene            complement(12398..13594)
                     /locus_tag="VD791_04550"
     CDS             complement(12398..13594)
                     /locus_tag="VD791_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV07273.1"
                     /translation="MALDPVASPKGKLVVRNIGLLLSGDLDRPILEADTVVAVDGMIT
                     QVGKEKDCDTAQPDTLIDAKGVCLAPGLIDSHVHPVFGDWTPRQNQLGWIDSTLNGGV
                     TTMISAGEVHLPGRPKDIVGLKALAITAQRAFHNFRPGGVKVLAGAPVLEKGMTESDF
                     RDLAQAGVTLLGEVGLGSVKAGAEARQMVAWARKHGIQSTIHTGGPSIPGSGLIDKDV
                     VLEADADVIGHINGGHTALPEAHVCELCERSSRAIEIVHNGNEKVAVAAAQAARDRGC
                     LERVILGTDGPAGSGVQPLGILRMIALLSSLGAIPAEQVFCFATGNTARLRKLDSGLI
                     APGRAADFVFLDRAQHTAGRTLLESVALGDIPGVGMVVIDGSVRCGRSRNTPPATEIP
                     VVVDAG"
     gene            13684..14367
                     /locus_tag="VD791_04555"
     CDS             13684..14367
                     /locus_tag="VD791_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXV07274.1"
                     /translation="MRILLIEDDTDIAANLYDYLESKGHDVDAAADGITGLHLAVTHD
                     FDAVVLDLALPGLGGLQVCRKLREEAKRDTPLLMLTARDTLEDKLEGFAAGADDYLVK
                     PFALSEVEARLQALNKRHRGRVTSRPLQAGGVVFDPATFTVERDGHPIKLPPKCLRLL
                     ELLMQQPQKVFSRAELESAVWGGEHPSSDTLRSHMHILRRALALPGKPDVIETVHGIG
                     YRFVANRGH"
     gene            14357..15676
                     /locus_tag="VD791_04560"
     CDS             14357..15676
                     /locus_tag="VD791_04560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXV07275.1"
                     /translation="MGIKHSLRLRVAFGIALLSIVVVGAHSTALYLATNRAEEAEIDR
                     IITEEIEHLIDRHRANPAAVPPHTQRLAGYIAHGDAELDQVPPHLRHLPQGLREVFVD
                     DNEFHVAVRIDGPAHFFIAYDAEHHEQRMHDFMLLLLFGVSATAVVAIVLGYWLAGFL
                     VDPVTDLAARVGTLEPGAKDIGLATSYADDEVRRLARAFDRYHARMAEFVEREQQFTA
                     DVSHELRTPLTAIRTSCELLGLDPSLAPGSRKRLEQIARAARRMSDLVSSLLLLARTG
                     QTAEPEDVSLRESIDEACDPLRETAARKGIAVDIAVDPKVILRLDRNALDLILSNLLR
                     NALAHTDVGCVQIRFREGTLTIADTGCGIEPEDIPHLFRRFYRGAASPSRGEGFGLGL
                     ALVKRLCDLYGWQVAVDSAPGKGTRVIIAFPPGVVHSQEIDLSLTRP"
     gene            15722..16510
                     /locus_tag="VD791_04565"
     CDS             15722..16510
                     /locus_tag="VD791_04565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV07276.1"
                     /translation="MPHEVLDRNQRGSGQVEVSVVVPVRNEAENIEPLLGEIRVALDG
                     RLAYEVIYVDDGSTDTTAARLLAAAAEFPRLRVIRHVEPCGQSAALWSGVRAARAAWI
                     ATLDGDGQNDPADIPRLLAVLRDSSAPQTLRLVTGHRRKRRDSALKRLSSRIANGVRA
                     RLLDDRTPDTGCGLKLIRRETYLALPFFDHMHRFLPALVQREGGATVSVEVMHRPRTR
                     GRSNYGMHDRLWAGIVDLIGVLWLKRRMKHPQIADGAVSNGDAA"
     gene            16500..17246
                     /locus_tag="VD791_04570"
     CDS             16500..17246
                     /locus_tag="VD791_04570"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HXV07277.1"
                     /translation="MQPEPPSSGRDWRTVLRGAMLLGSLAVFGLLLQRTHVGAMFGPG
                     WVDAPAWIDAEVRGHGVAGELLFVCVAGMLTAVGVPRQLVSFLGGYAFGLVFGTVLAV
                     AATLLGCAASFYCARVLGRAYLARRFSGRVRRFDDFIRAHPLGMTLLIRLSPVGNNLL
                     TNLLAGVTSVRPSLFFAASAVGYIPQSLAFALGGSGVEVDPLLRTALAVILFLVSGAL
                     GAWLLRKFRRGPPLDFDTESELTYASGPRA"
     gene            17372..18856
                     /locus_tag="VD791_04575"
     CDS             17372..18856
                     /locus_tag="VD791_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXV07278.1"
                     /translation="MGGWFVLLATALWLRPLWPVDETRYLSVAWEMWLRADFLVPYLN
                     GAPYSHKPPLLFWLMQAGWALFGVNEWWPRTVAPLFALASLFLTALLGRRIWPQRAEC
                     GETAAWILFGSLLFALFATLTMFDVLLTGFALLGVHGVLRASQGQVLRGFAQLATAVG
                     LGALAKGPVILLHLLPVALLAPWWMAQPRPPAARWYLGVVAAALLGGGIVLSWALPAG
                     IAGGSAYRDAILWGQTAGRMAHSFAHPHPWWWYLPMLPAILFPWPLWPPLWRALNRLR
                     TAPSDPGVRLLLAWIVPPFIGFSLISGKQPNYLLPLVPGVALLAAHALHAFARVDLLN
                     RVRLLAAASPALVLAINAGIIRNVGVDHDLHPIGDHLRALQQSGVPIAHVGSYLGQFH
                     FVGRLTRPIEVVEPEQVQRWVEGHPCGRLIGYRDAAPARAARGSVPGSPSTRSYPLGE
                     PEIIQGYRRRQVFVWRPGPICAADAVRWARRPQPALIRRSRPAY"
     gene            complement(18828..19787)
                     /locus_tag="VD791_04580"
     CDS             complement(18828..19787)
                     /locus_tag="VD791_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXV07279.1"
                     /translation="MQTEVPEVPWQPSQRYPDAAVRVLDQSFARYRLNLAAVERIAHG
                     FRWAEGPVWFGDGRFLLWSDIPNNRIMKWEEETGAVGVFRKPSNHANGNTRDRQGRLI
                     TCEHGGRRVSRTEYDGTVTAVADSFEGKPLNSPNDVVVKSDGSVWFTDPPFGLLGWYE
                     GYPAKQELPMNVYRVDARGEMTVVESDVNRPNGLCFSPDESILYLVESGTSPRNVYAY
                     DVVDGGRRVANRRKLIDVGPGTPDGMRCDADGNLWMGWGMGEAALDGVAVFNPDGRLI
                     GRIDLPERCANLCFGGLHRNRLFMAASKSVYALYVNTQGASGG"
     gene            complement(19900..>20179)
                     /locus_tag="VD791_04585"
     CDS             complement(19900..>20179)
                     /locus_tag="VD791_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV07280.1"
                     /translation="LTVARTLMGNPLLVLLDEPSEGIAPRIVEEMANMILELKREGLS
                     VLLCEQNLHFAALVSDRAYVIEKGEIRHSGTMRELAANDAVKREHLSL"
BASE COUNT         2890 a         7044 c         6879 g         3366 t
ORIGIN      
        1 accgccgcag aataacaccc ctgaggagac cccggtgacc gtcccagcgc tgactcccgc
       61 ctcgtccgcc cgccgtcgct ttctcgtcga cgcgggggcg ctcgccgccg gcgccctgat
      121 ggccggcgcc gctcgcgccg cccgcatcaa cccgcccgcg ctgctcgaca accaagccgg
      181 cggctatcgg gtgctgccgg cgggacaggt gttctgcggc ggcgtgattc cgaaccccgg
      241 gtacgagatc gtgcacgcac tgttcaatcc gtgggtgccg ctggacgacg cgtggggcct
      301 catcgaggcg cacctgaagt cgctcggccg gccggtgcag gcgctgtgcg ggatggagct
      361 gcgcatcccg cagcagctca cggtggaggg tttccgcgcc ttcaacgcgc cctacgtcga
      421 gcacttgcgc aagcgcgacc tgctgctggg caactacagc gccgtgtgcc gcaccaacgt
      481 cgcgcccggc cgcgacgcgc ccaaagtgcc ttcgctgcac gccttctcct attgcctgcc
      541 cggcgcccac accggcacca ctttttgcgt atcgggcacc gccgacatcg acgcgcgcgg
      601 caagatcgtc gccgaaggcg acgtgtcggc cgccggcatg cgcgcacgcc tgcagcactg
      661 cgtggacgtg atcaccgagc gcctcgccat gctggagctc gactggagcg ccgccacgca
      721 catcgatcta tgcgtggtgg aagacatccc cgggctgatg gacgcggtgg tggtgccggg
      781 actgcaaggc gcggcgtcgc gcggcgtgcg cgtgcatgtc gcacgcccgc ccatcatcgg
      841 cgccgaggtc gagctggagt gccgcggcgt gcgtcaggag ctggtgctgc gggcatgacc
      901 cgccttcgag gtcgccggct gccctcgatc tcgacgacgg ctagagcgac tcgaggaagc
      961 gcagcagcgc ctcccgctcg gtcccgggca tcgccttgaa cgcgtcgcgc gaggcggtcg
     1021 cttcgccgcc gtgccacagg attgcttcga ccagcgtgcg cgcacggccg tcgtgcaggt
     1081 agttgacgtt gccgttcacc gtctgcgtca ggccgatccc ccacagcggc ggggtgcgcc
     1141 actcgcgtcc gctggcctcg aagtcgggcc gctcgtcggc gagcgcttcg cccatgtcgt
     1201 gcagcagcag atcggtgtaa gggcggatca cctgctgcga taacgcgggc tggcccggta
     1261 cctcgccggt gcgcagctcc ggcacatggc aggcggcgca ctgcgcctgc gcgaacagca
     1321 tctcaccgcg tcgaacgccg gcatcgtcgg gattgcgacg cgccggcact gcgccgaagc
     1381 gcagcaggga ctcgacggcc ttcaggcggt actcggtgat ctcgggtctg cccgccacca
     1441 tcatttcgcg gcagtactgc tgtgcgcccg ggcagttctg ctcgggaaac agctccgtgg
     1501 acaggccgat gtcctcgatg aacgccgccg ccacctgctg gcgcagcgtc gggtggttgg
     1561 ccttcaggcc gaagcgcccg agcgcgaagc gcttgccctc cacgtcccac acccgattcg
     1621 gccttccgct gatcccttgc ggcgcggccc gcgccgcgag cgcgaggatg acctcctccg
     1681 gcaccgcatc gagcaggccc atgccgatga cgggcggggc gaccctcgcg gaaatcatga
     1741 tgtcgtcgcc gagcggtccg tacgcgagct tctcgatccg cacccgcggc gcgcgcagct
     1801 cgacggtctc gccgtccgcg aagcgctcgg catgcgtgct ccagtcgaag gcgatgcgcg
     1861 cctcgggcgc gatcaggcgc ggcacgccct gggtctgcag ctgcgcgccg tagttcggat
     1921 cgggccgcgg cccgccgttg gcgtccttcc cgggcaggcc gatctggacc acgaaggtgt
     1981 cgtcgcggtc gccctcgcgc ggcggcgcgc cgcggccgtt cgcgcgatgg caggtggcgc
     2041 acgcgtccgc gttggaggtg ggtcccctgc cccacaggcc ctgctcgaac cagtagaacg
     2101 cccagcgcgt gtcgtagaac gtgcgcccgt cggcgatctg ccgatactcc gcctggctga
     2161 gcgtgagcga gagctgctcg taggcgcgcg gtccgctctc cgcggtggtg agcgcgccgc
     2221 cggcgagcgc gagcgactgc ggcgcgtcgc cgtccgcagc gatcaacgca ccggccagaa
     2281 tcaaccccac ggggcccagc gtcagcgctg caggtcgcat cgcgcgcacg ttcgtctccc
     2341 ggtcaacgag cctgccgaat tcttgcataa tcctcgatca tgttcgacga ccccctgctg
     2401 ctcgcccgca cccagttcgg gctgaacatc gcgttccaca tcctgttccc cagcatcacc
     2461 atcgcgctcg cctggttcct ggtgtacctg cgcgtgcgcg ccgaccgccc gggcgcgggc
     2521 tgggcgcaga aggcgtacga gctgtgggtc aagatcttcg ccctgtcctt cgcgctgggg
     2581 gtggtgtccg gcatcgtgat gagcttccag ttcggcacca actggcccgg cttcatgcaa
     2641 aaagcgggca acgtcgcggg gccgctgctc ggctacgagg tcctcaccgc gtttttcctg
     2701 gaggcgacct tcctcggggt gatgctgttc ggccgcgagc gcgtgccgcg ctggctgcac
     2761 accctgtcag ccgtgctggt ggccgtcggg accaccggct cggcgttctg gatcctggcg
     2821 ctcaactcct ggatgcagac gccgcagggc ttcgaagtga tcgacggcgc cttgcacgtg
     2881 gtgagctggt gggacgtgat cttcaatcca agttttccct accgcttgac ccacatgctc
     2941 ctggcctccg ggctcacggc ggcgttcctg atcgccggcg tatcggcctt ccagttgctg
     3001 cgcaatcacg cgcaccagcc cgcctggcgc atgttccgcg ccgggaccgc ggtcggcgcg
     3061 gtcttgatcc cgatccagat cgcagtcggc gatcttcacg ggctcaacac gctcgagcat
     3121 cagccggcca agatcgcggc gatcgaaggc atctggacct ccggcgcgcg cgtgccgctg
     3181 gtgctgctcg cctggccgaa ccccgagcag cggcgcaacg attacgccgt cgagattccc
     3241 ggcggtgcca gcttcatcct gcgccacgac tggaacgccg agctggaggg gctggatcgg
     3301 ttcgcggacg agcatccgcc ggtgcgcccg gtgttcttcg cctttcgcgt gatggtcggc
     3361 accggcgtgc tcatgctcct gctgtcgtgg ctggcgagcg gctacctgct ggcgaagcgc
     3421 cgtatcccgc cgtggatgct ggtcgcgttt gcgctgatga ccttctccgg ctgggtggcg
     3481 acgctggccg gctggctggt gaccgagatc gggcggcagc cctatctggt atacggcgtg
     3541 atcaccacgg cggagacggc ctccagcgtg ccggcggcca acatcgcgat caccctgatc
     3601 gggtacgcgc tggtgtacgc ggcgctgctc atctcttacc tcgtggtgct cacgcaactg
     3661 gcgcgcagcg ccgccggcga tgcgccggtg atcccgccct cgggcgagct gcagcccacc
     3721 gggacggtgc gcgcatgagt ctggatctgc cgttgatctt tgcgatcttg atgggcctgg
     3781 cgatcctggc ttacgtgatc ctcgacggtt tcgatctggg cgtgggcatc ctgctgccgg
     3841 gcggccagcg ccccgagcag gacgtgatgg tgtcctccat cggcccgttc tgggacgcga
     3901 acgaaacctg gctggtgctc ggtgtcggca tcctgctggt cgctttcccg ctggcgcacg
     3961 gcgtggtgct gtcctcgctc tacctgccgg tggcggcgat gctgtgcggc ctggtcctgc
     4021 gcggcgtggc tttcgagctg cgcttcaagg ccaccggctg gcaccgggag gtgtggaatc
     4081 gcctgttctt cgtcgggtcc ctgctcgcct cggtggcgca gggagcaatg ctcgccggcg
     4141 tgatcaccgg cttcgcgagc ggaccgtggt tcacgctgtt cggcttgctg gtcgggctcg
     4201 gcctggcgtg cggctacgcg ctgctgggcg cgacctggct ggtgatcaag accgagggcg
     4261 agttgcgctc gaaggccctg cgctggagcc ggctggcgct gatcggcgcc gccgccggcg
     4321 tggcggtcat cagcctcgcc acgccgctcg ccagcccgcg catcctgcac aagtggttcg
     4381 actggccacg catcgcgttc ctcgccccgc tgccgctgct caccgcggcc gcctgcttcg
     4441 gcatctggag ctgggtgggc cgcttgcaat cgggcaggac gcgcctcgag tggccgccgt
     4501 tcgtgctggc ggtgtgggta ttcgtgttcg ccttcgccgg cctggcctac agcctgttcc
     4561 cctacctcgt catcgaccgc ctcaccgtgt ggcaggcggc ggccgccgag aagtcgttgt
     4621 ggttcgtgtt catcggcgcc tgcgtcgtgc tgccgttgat cgtcgcctac acgctgctgt
     4681 cctaccgctt gttctggggc aaagcgcgca ccctcacgta cggaccttga gggccaccga
     4741 ccgcttgccg cagacctgtc tgcgctgcct gctgctcccc agagccggca cgctgttggc
     4801 ttgtacggac cggtcgattg gagtctaatt gtccggattc gtgcatcttt cgccccccgc
     4861 gcacgtggac ccgctgcccc cagaacgcgc cgtcgaacag gccatcgccg cgcaccggca
     4921 tcgccccggt gcgctgctgc cgatcctgca tgcggtccag gacgcgatcg gttacgtgcc
     4981 gccgagcgcc atcggtcaga tcgcgcacgc gctcaacctg tcgcgcgccg aagtgcacgg
     5041 ggtcatcagc ttctatcacg acttccgcac ctcgcctccg gggcggcacg tggtgcgcgt
     5101 gtgccgcgcc gaggcctgcc agtcgatgga cggcgtcgcg ctcgagcagc acgcgagggc
     5161 gcggctcgga accggctggc atcagaccac cgaagacggc gcggtcacgc tggagccggt
     5221 gtactgcctg ggcaactgcg cgtgctcgcc ggcggtgatg atcgacggaa aggtgtacgg
     5281 gcgggtaacg ccggcacgct tcgacgcgct gctggacgcc gctcgaggca gtgcaccgtc
     5341 ggccgcgcgc tctccggccg agcgccctga ggcgatgccg gcggcggacg acgccgtcac
     5401 cgttctcgtt ccgcgcgaca cgagcgccgt ctcgctgggc gccgatgccg tcgccgatga
     5461 gatcgcgcgt cttgccgccg agcggcgcac gccggtgcgc atcgtgcgca acggttcgcg
     5521 cggcgtgttc tggctggagc cgctggtgga agtgcagcac gctgccagcg gacgcgtagc
     5581 ctacggcccg gtgcagcccg aggacgtgcc cgggctgttc gacgccggct tcctgcacgg
     5641 tggcgcgcac cggctcgcgc tcggcccgac cgccgagatc gattacctga aaagccagga
     5701 gcggctcacc ttcgagcgct gcggcgtgat cgacccgctc agcctcgagg actacgcggc
     5761 gcacggcggt tggcaagggc tgcaccacgc ggtgacgatg caacccgcgg acatcgtcga
     5821 gcaggtcgtg cagtcgggtc tgcgcgggcg cggcggtgcg gcgttcccgg ccggcatcaa
     5881 gtggcgcacg gcgctcaatg cgcccgccga ccggaagtac atcgtctgca acgccgacga
     5941 gggcgactcg ggcaccttcg ccgaccgcat gctcatggaa ggcgatccct acctgctgat
     6001 cgaaggcatg atcatcgcgg gactcgccgt gggcgcgacg gccggctaca tctatctgcg
     6061 ctctgagtat cccgccgccg agcgggtgct ggaacaggcg atcgaccgag cgcgcgccgc
     6121 gggatatctc ggccacgacg tgatcggcgg cggcaaagcc ttcgagctgg aagtgcggcg
     6181 cggcgccggc gcctacatct gcggcgagga gacctcgctg ctggagagcc tggagggcaa
     6241 gcccggccag gtgcgcttca agccgccgtt gcccgcgatc cagggcctgt tcggcaagcc
     6301 cacggtggtc aacaacgtgg tcacgctggc cagcgtgccg atcatcctcg agcgcggcgc
     6361 tgctttctac cgcgacttcg gcacgggccg ctcgcgcggc acgctgacta tccagctcgc
     6421 cggcaacatc cggcgcggcg gcctggtcga gaaggccttc ggcgtgacgt tgcacgagct
     6481 gctgtacgaa tacggtggcg gctccgccag cggccggccg atccgcgccg tgcaggtggg
     6541 cgggccgctc ggcgcgtatc tgcccgagtc gcagttcgac acgccgctcg actacgaggc
     6601 gttcgcggcg ttgaacgcga tgatcgggca cggcggcgtg gtggccttcg acgaccgcgt
     6661 ggacatggcg cacatggccc gctacgccat ggagttctgc gcggtagaat cttgcggcaa
     6721 gtgcacgccg tgccgcatcg gttccacccg cggcgtggag gtggtggagc ggatcgtgcg
     6781 cggcgagcgc cggcccgaga accacgcgct gctgcgcgac ctgtgcgata ccatgctgca
     6841 cggatcgctg tgcgcgctgg gcgggatggc cccttacccg gtgctgagcg cgctgaatca
     6901 ctttcccgag gatttcgggc tggcacccgg agtcaagggc gcttaggttc gcctgacgtt
     6961 agggccggcc tgctggagga agcatgtact cgatcaagga actcgactac ggtacgccca
     7021 ggcgtgtctc cggcgcgccg gtgacgctgg agatcgacgg caagccggtc accgtggcga
     7081 gcggctcttc ggtgatggcg gcggcggtcg aggccggcgt gcgcgtgccc aggctgtgcg
     7141 ccaccgacat gctcgaggcg ttcggctcct gccgcctgtg tttggtggag atcgaaggcc
     7201 gcaagggcta tcccgcctcc tgcaccacgc tggtcgagcc cggcatgaag gtgctgacgc
     7261 agacgccgaa gctcgcggac ctgcggcgcg gcgtgatgga gctgtacatc tccgatcatc
     7321 cgctcgattg cctcacctgc cccgccaacg gtaactgcga gctgcaggag atggccggcg
     7381 tggtcggcct gcgcgaggtg cgctacggct acgacggcgc caaccatctg cgcgccgcga
     7441 ccgacgcctc caacccctac ttcaacttcg atccgaccaa gtgcatcgtc tgctcgcgct
     7501 gcgtgcgcgc gtgcgaggac gtgcagggca ccttcgccct caccgtggac gggcgcggtt
     7561 tcgcctccgt gatctcgccg ggcggcagcg atttcctggc ctcggagtgc gtctcctgcg
     7621 gggcctgcgt gcaggcctgc ccgaccgcca cgctgatgga gaagtccatc gtcgaacgcg
     7681 gccagcccga gcatagcgtg atcaccacct gcgcctactg cggtgtgggc tgttcgttcc
     7741 gcgccgagat gaagggcggc caggtgatgc gcatggtgcc cgacaaggac ggtcacgcga
     7801 accatggcca ttcttgcgtg aaggggcgct tcgcctgggg ttacgccacg caccccgatc
     7861 ggatcttgaa gccgatgatc cgggaaaaga tcacggaccc gtggcgcgag gtgtcctggg
     7921 acgaggcgat cgcgcacacc gccgccgagt tccagcgcat ccagcgtgag cacggacgcg
     7981 gtgcggtggg cggcatcacc tcctcgcgct gcaccaacga ggaaacctac ctggtacaga
     8041 agctggtgcg cgccgccttc cgcaacaaca acgtggacac ctgcgcgcgc gtgtgccatt
     8101 cgccgaccgg ttacgggctg aagcaaaccc tgggcgagtc cgccggcacg cagaacttcg
     8161 attcggtggc gcacgcggac gtggtgatgg tcatcggtgc caatccgacc gagggtcatc
     8221 cggtgttcgc ctcgcaattg aaacgacggc tgcgcgaggg ggccaagctg atcgtcgtcg
     8281 atccgcgcac catcgacctg gtgcgccagc cgcacgtcga ggccgcctat cacctgcagc
     8341 tgcgcccggg caccaacgtc gcagtgatca acgcgctcgc ccacaccatt ttcaccgaac
     8401 ggctgtggaa cgaagcgttc gtgcgcgcgc gctgcgatct caaggaattc gagaagtgga
     8461 aggcgttcat cgtgcagccg tacaactcgc cggaagcgat ggagccgatc accggcgtcc
     8521 ccgcggccga cctgcgcggc gcggcgcgcc tgtacgccac cggcggcaac ggcgccatct
     8581 actacggcct gggcgtgacc gagcacagcc agggttcgac catggtgatc ggcatcgcca
     8641 acctcgccat gctgaccggc aacctgggac gcccgggcgt gggcgtcaat ccgctgcgcg
     8701 gccagaacaa cgtgcagggc tcctgcgaca tgggctcctt cccgcacgag ctgcccggct
     8761 accgccacat ctcggatgcc accacccgcg cgttgttcga ggcggcctgg ggcgttacgc
     8821 tggagagcga gccgggtctg cgcattccca acatgctcga cgcggcgctg gacggctcgt
     8881 tcaagggcct gtacgtgcag ggcgaggaca tcgcccagtc cgatcccgac acgcagcacg
     8941 tcaccgcggc gttgagtgcc atggaatgcc tggtggtgca ggacctgttc ctgaacgaga
     9001 cggcgaagtt cgcccacgtg ttcctgcctg gctcgtcttt cctggagaag gacggcacct
     9061 tcaccaacgc cgagcgtcgc gtctcgcgcg tgcgcaaggt gatggcgccg ctttgcggtc
     9121 tggccgactg ggaggcgacc gtggcgctgt cgaacgcgct gggctatccg atgcattacc
     9181 gccatccgtc ggagatcatg gaggagatcg cgcggctcac gcccaccttc cacgggatga
     9241 gctacgccaa gctcgaccgc ctgggcagca tccagtggcc gtgccacgag cgcagcccgg
     9301 agggcacgcc ggtgatgcac gtcgacgagt tcgtgcgcgg caagggcaag ttcatgctca
     9361 ccgcgtacgt gccgaccgac gagcacacct cgcgcatgtt cccgctgctg ctcaccaccg
     9421 gccgcgtcct gtcgcagtac aacgtcggcg cgcagacgcg ccgtacggcc aacctggtgt
     9481 ggcacgacga ggacaagctc gagatccatc cgcacgacgc cgagcagcgc ggcgtgcgcg
     9541 agggcgactg ggtgggcgtg cgcagtcgcg ccggcgagac ggtgctgcgc gcgcatgtga
     9601 ccgagcgcat ccagccgggc gtggtctaca ccaccttcca ccacccgttc tcgggcgcca
     9661 acgtggtcac caccgacatc tccgactggg ccaccaactg cccggagtac aaggtgacgg
     9721 cggtgcaggt gacgcgcgtg agccaaccct cggagtggca gcgccacttc accgagatcg
     9781 atcaccagca gcacgagctg ctcgagcacc ggcaacggga gcgggcctga tccggcgggg
     9841 tgcgatgtgg acccggagaa gctgatccac atggccaacc gcatcgccga cttcttccag
     9901 gccgagcccg accggcaggc ggcgctggag ggtatcgccg gacacctgaa gcgcttctgg
     9961 gacccgcgca tgcggcgcga gatcctgcgc ctggtggatg aggatcgggc gcaaggcctg
    10021 tcccctctgg tggtcgacgc cttgcacgct caccgcaagc tgatcgcgcc cggcgagctg
    10081 cgcgcctagg cctgctcagc cggccaccgc gcccacgcgc gcgatggtcc acgccagtcc
    10141 cgccgccagc acgatggcgg acagcagtcg ccgcagccat ggcgaccacg cgaacgcgtc
    10201 ggcgcgcttg agcaacggcc aggcaagcac cacgagtacg agctggaacg cttcgattcc
    10261 gaggttgaag ccggcgagcg cgaccagcca tcccttgtcg gtgacgccgg acagggccgc
    10321 gctcagcgcg ccggcgaagc cgaagccgtg catcagaccg aaaccgagcg cgatccacgg
    10381 cccgtgcggc gtgtgcgggc gcgctagcgt caccgcgccg acgtaggcga tgctggcggc
    10441 gatcagaagt tcgaccagcg cgccgggagg cgtgatccag ccggccgcgg ccaccgcgag
    10501 cgtcacgctg tgcgcgagcg tgaacgccgt gaccaccgcc gccagccgac ccagcgactg
    10561 gcggaacagc aggagcccga gcaggaacag cagatgatcg tacccggtcg cgatatggtg
    10621 cacgcccagc ttgaagaacg gccacgcgta gtggcgccag gcgctcgccg gcggcgatgc
    10681 gctcggtgcc ggcgcttgcg catgcgcctg cagacgaccg ctgtcgtccg ccgagggctt
    10741 cgggccgcgt tcggcgctct ccggccgagg taccggagcg ggggccgcgc ggatcggcgc
    10801 ggccgtctcc ggctttgctc gcgcccctcg ggcgtggtcc gcagcctgcg cggccggcac
    10861 tggcagcgtt gccggcgcat ccggcgcgcg cgccgtctcg gcaaccggtg cggcggcgaa
    10921 gcgcagcagc gcctgcgccg ccgtctcgtt cggattcggc agctccgaga tcgcggcgta
    10981 gtcggtcagc tcatagctgc gatcatgcga cggcacgctc gcccgtgcga tgccggaaga
    11041 tccgccggcg gtgagcatca cctccttcca gccgacctcc tctgggcgca gatcgtcgcg
    11101 gtagtcgagt gtgccggtcg cggccgtcac cggcaggcgg gcgccgaagc gcacaaccag
    11161 cttccatgtt gcgagcccca tcgcgccttc gcccagctcc cagcgcacgc cttccagcgt
    11221 caagggcacg agcgcgtcgt cgagccgcac gtgcaggtgg cgcaacagct cctggcccag
    11281 gctcgccgcg tacgcgtcgc gctcggcggc cgaggtcgag ccgtcgctgt cagcgtccca
    11341 tgcgcgcgcg gtgttgatcg ccgcgatttc cagcatctcg tagagatagc ggatctcgag
    11401 gcggtcgtcg gtgacccgca gggtcacgtg acggttgatg tcggtgctga cgatcgggtg
    11461 cgcgttggca gagggcgcgc agatcgcgca cgcgaggaac cccgccacgg cggtcgcggc
    11521 aagccgccgt gggcggttgc tgcgcggctt cagccgaact ggagatcggt ggacatggcg
    11581 cgcacgacgc cggccttttc catttccttc cagcccgctt ccatggaacc gcgcacgtcg
    11641 atgccgcgcg aagcgtcctc cagcaccacc acctggaagc cgtccttgcg cgcatccacc
    11701 gaggaccaca ttacgcagaa atcggtggcc agaccgcaca cgaacacgcg cttgacgccg
    11761 cgcgccttca tatagccggc gaggccggtg gtcgacttct tgtcgttgtc atagaaggcg
    11821 gagtagccgt ccaccttggg gttgaagccc ttgcgcagga tcaggttggc cttggtgagg
    11881 tcgagctgcg cgtgcagctt ggcgtcgtcg gtgccctgga cgcagtgatc gggccacatg
    11941 acctgtttgc cgtacggcat gtcgatcacc gagaacggct ccttgccgtg cgtggaagca
    12001 aacgagatgt ggcccgcggg gtgccagtcc tgcgtcatca ccacgcagtt gaacttgccg
    12061 gccagctcgt tgatgagcgg caccacctcc tcgcccttgg gaaccggcag cgtgccgccc
    12121 gggatgaagc agttctgcac gtcgatcacc agcagcgcgt cgctggggcc gaccttgatc
    12181 gcgcccgccg ccagcgcgtg gcgcaggccg gccagcggca gcgcggcgga cgccgttacg
    12241 gcaccggcag ccatgatgaa gttgcgtcgg gaaagggtgc tcatcggtct cctcttctga
    12301 taacgatgga atcatgggtg cgccggcagg gccagtggcc accgtgcact gtccagtgta
    12361 gcaccggccc ggtgtggatc aagggcgcgc gcggcggtca gccggcatcc accaccacgg
    12421 gaatctcggt cgcgggcggg gtgttgcggc tgcgcccgca gcgcacgctg ccgtcgatca
    12481 ccaccatgcc cacgccgggg atgtcgccca gggcgacgct ctccagcagc gtcctgcctg
    12541 cggtgtgctg cgcgcggtcg aggaacacga aatcggctgc acgcccgggc gcgatcagcc
    12601 cgctgtcgag cttgcgcagg cgcgcggtgt tgccggtggc gaagcagaac acctgctccg
    12661 ccgggatcgc gccgaggctg gacagcagcg cgatcatgcg caggatgccg agcggctgca
    12721 cgcccgatcc tgccggtccg tcggtcccca ggatcacgcg ctctaggcac ccgcggtcac
    12781 gggcggcctg cgcggcggcc accgccacct tctcgttgcc gttgtgcacg atctcgatgg
    12841 cccgcgaaga gcgctcgcac agctcgcaca cgtgcgcctc cggcaacgcc gtatggccgc
    12901 cgttgatgtg gccgatgacg tcggcgtcgg cctccagcac cacgtccttg tcgatcaggc
    12961 ccgagccggg gatcgacggc ccgccggtgt ggatggtgct ctggatgccg tgcttgcgcg
    13021 cccacgccac catctgccgc gcctcggcac cggccttgac cgaccccagg ccgacctcgc
    13081 ccagcagcgt cacgcccgcc tgcgccaggt cccggaagtc cgattccgtc atgccttttt
    13141 ccagcaccgg tgcgccggcg agcactttca cgccgccggg acggaagttg tggaacgcgc
    13201 gctgcgcggt aatcgccagc gccttcaggc cgacgatgtc cttgggccgc cccggcagat
    13261 gcacctcgcc agccgagatc atcgtggtga ccccgccgtt caacgtcgag tcgatccagc
    13321 cgagctggtt ctgccgcggc gtccagtcgc cgaacaccgg atgcacgtga ctgtcgatca
    13381 ggcccggggc cagacacacg cccttggcgt cgatcagagt gtcgggctgg gcggtgtcgc
    13441 agtccttctc cttgccgacc tgcgtgatca tgccgtcgac cgcgaccacg gtgtcggcct
    13501 ccaggatcgg acggtcgagg tctcccgaca gcaacaggcc gatgttgcgg acgacgagct
    13561 tgcctttggg cgaggcgacg gggtcgagtg ccatgggtca cggagccatc ctgcacgcat
    13621 actgtatcgc agcacggcgg ctttgtcgta tagttgccgg cgttttccgc caggacctcc
    13681 gccgtgcgca ttctgctgat cgaggacgac accgacatcg cggccaatct ctacgactat
    13741 ctggagtcca aaggccatga tgtggacgcc gcggccgacg ggatcaccgg cctgcacctc
    13801 gcggtgacgc acgatttcga cgccgtggtc ctcgacctcg ccttgcccgg cctcggtggc
    13861 ctgcaggtct gccgcaagct gcgcgaggag gcgaagcgcg acacgccgct gctgatgctg
    13921 accgcacgcg acacgctgga ggacaagctc gaagggttcg ccgccggcgc ggacgattat
    13981 ctggtcaagc cgttcgcgct gagcgaggtc gaggcgcgcc tgcaggcgct gaacaagcgt
    14041 catcgcgggc gcgtgaccag ccggcccctg caggccggcg gcgtcgtctt cgacccggcc
    14101 acgttcaccg tcgagcgcga cggccatccc atcaagctgc cgcccaagtg cctgcgcctg
    14161 ctggagctgc tgatgcagca gccgcaaaag gtgttcagtc gcgccgagct cgagtccgcc
    14221 gtgtggggcg gcgagcaccc gagcagcgac accctgcgca gccacatgca tatcctgcgc
    14281 cgcgctctcg ctctgccggg taagccggac gtgatcgaga ccgtgcacgg aatcggctac
    14341 cgcttcgtcg ccaatcgtgg gcattaaaca cagtctccgc ctgcgggttg cgttcgggat
    14401 cgcgctgctc agcatcgtcg tggtcggcgc gcacagcacc gcgctgtacc tggccacgaa
    14461 ccgggcggaa gaggcggaga tcgatcgcat catcaccgaa gagatcgagc acctcatcga
    14521 ccgtcatcgg gcgaacccgg ccgcggtgcc gccccacacc cagcgcttgg ccggttacat
    14581 cgcgcacggc gatgcagagc tcgaccaggt tccgccgcat ctgcgccatc tgccgcaagg
    14641 gctgcgcgaa gtattcgtgg acgacaacga gtttcacgtg gccgtccgta tcgacgggcc
    14701 cgcgcacttt ttcatcgcct acgacgcgga acaccacgag caacgcatgc acgacttcat
    14761 gctgctgctg ctgttcggcg tgtcggcgac cgccgtggtc gcgatcgtgc ttggctactg
    14821 gctcgccggg ttcctggtcg atccggtgac ggacctcgcg gcgcgcgtcg gcacgctgga
    14881 acccggagcc aaggacatcg gcctggcgac ctcctacgcc gacgacgagg tccggcgcct
    14941 ggcgcgggcg ttcgaccgct atcacgcgcg gatggccgag ttcgtcgaac gcgaacagca
    15001 gttcaccgcc gacgtcagcc acgagctgcg tacaccgctg acagcgatcc gtacgagctg
    15061 tgagctgctg gggctcgatc cgagcctcgc gcccggctcc cgcaaacgcc tcgagcagat
    15121 cgcccgagcc gcccggcgca tgtcggatct ggtgagctcg ctgttgctcc tcgcccgcac
    15181 tggccagact gcggagccgg aagacgtgtc gctgcgggag agcatcgacg aagcatgcga
    15241 cccgctgcgc gaaacggcgg cgcgcaaagg gatcgccgtc gacatcgcgg tcgatcccaa
    15301 agtgatactg cgtctcgatc gcaacgcgct cgatctgata ctgtccaact tgctccgcaa
    15361 cgcgctcgct cataccgacg tcgggtgcgt ccagattcgg ttccgggagg ggacgctcac
    15421 gatcgccgac accggctgtg ggatcgagcc cgaggacatt ccacacctgt tccggcgctt
    15481 ctaccgcggg gccgcctcgc catcgcgcgg cgagggcttc ggtctgggac tggcgctggt
    15541 gaaacgcctg tgcgacctgt acggctggca ggttgcggtg gacagcgcgc ccggcaaggg
    15601 cacccgcgtc atcatcgctt tcccgccggg cgtggtgcat tcacaagaaa ttgatctctc
    15661 attgacccgt ccttgaccct accccctgta ggctgtcgtg cggacagcgg ctcacggtgg
    15721 cgtgccgcac gaggtactgg atcggaacca gcgcgggtcg ggtcaagtgg aggtatcggt
    15781 agtcgttccg gtgcgcaacg aggcggagaa catcgagccg cttcttggcg aaattcgagt
    15841 cgcgctcgac ggccggctcg cgtacgaggt gatctacgtc gacgacggga gcaccgatac
    15901 gaccgcggcg cgcctgctcg cggcggcggc cgagtttccg cggttgcgcg tgatccgtca
    15961 cgtcgaaccc tgcgggcaga gcgccgcgct gtggagcggt gtgcgcgcgg cgcgcgcagc
    16021 ctggatcgcc acgctcgacg gagacgggca gaacgaccct gccgacatac cgaggctcct
    16081 ggccgtgctg cgcgattcga gcgctccaca gacgctgcgc ctggtcacgg gtcatcgccg
    16141 caagcggcgc gactcggcgc tcaaacggct gtcctcccgc atcgcgaacg gcgtgcgcgc
    16201 gcgcttgctc gacgatcgca cacccgacac cggctgcggc ctgaagctga ttcggcgcga
    16261 gacctatctg gcgttgccgt tcttcgatca catgcaccgg ttcctgccgg cgctggtgca
    16321 gcgcgagggg ggcgcgactg tctcggtcga ggtgatgcat cggccgcgca cgcgcggccg
    16381 ctccaactac ggcatgcacg accggctgtg ggccgggatc gtggacctca tcggcgtgtt
    16441 gtggctgaaa cgacggatga agcacccgca gattgccgat ggcgcggttt ccaacggcga
    16501 tgcagcctga gcctccaagc tcgggtcgcg actggcgcac ggtgctacgt ggcgccatgc
    16561 tgctcggttc gctcgccgtg ttcggactcc tgctccagcg gacacacgtc ggggcgatgt
    16621 tcggtcccgg ctgggtcgac gcccccgcct ggatcgacgc cgaggtgcgt gggcacggag
    16681 tcgcgggtga gctgctgttc gtgtgcgtcg cgggaatgct caccgccgtc ggcgtaccgc
    16741 gccagctggt gagcttcctc ggcggatacg cgttcgggtt ggtgttcgga acggtgctcg
    16801 ccgtggccgc cacgctgctc ggctgtgctg cgtctttcta ctgcgcccgg gtgctggggc
    16861 gcgcctatct tgcgcggcga ttctccggac gcgtgcgccg gttcgacgat ttcattcgcg
    16921 cgcatccgct tggaatgacg ctgctgatcc ggctgtcacc ggtggggaac aatctcctca
    16981 ccaatctgct cgccggcgtc accagcgtgc gtccgtcgct gttcttcgcc gcctcggccg
    17041 tcggctacat tccgcagagc ctggcgtttg cgctgggcgg cagcggcgtg gaagtcgacc
    17101 cgctgttgcg cacggcactc gctgtgatcc tgttcctggt gtccggagcg cttggcgcat
    17161 ggcttcttcg caagttccgc cgcggcccgc cgctcgactt cgacacggaa tcggaactca
    17221 cctacgcctc cgggccgcgc gcctagcacg gttcgaagcg atgctgaaag agtcgtcgcc
    17281 tcggagtctt tccctggttt ctgcctccct cgatccgccc gggccgagcc gcacccttcc
    17341 gcggctctct gagcgctatc tgccgctgct cgtgggcggc tggtttgtcc tactcgcgac
    17401 ggcactgtgg ctgcggccgc tctggccggt ggatgaaacg cgctaccttt cggtcgcctg
    17461 ggaaatgtgg ctgcgcgcag atttcctggt cccttacctc aacggcgcac cctacagcca
    17521 caagcccccg ctcctgttct ggctgatgca agccgggtgg gcgctgtttg gcgtgaacga
    17581 atggtggccg cgcacggtgg cgccgctgtt cgcgctcgcc agtctcttcc tcaccgcgct
    17641 gctcgggcga cgcatctggc cgcagcgcgc cgagtgcggt gagaccgcgg cgtggatcct
    17701 gttcggcagc ctgctgtttg ccttgttcgc gactctcacc atgttcgacg tcctgctcac
    17761 cggcttcgcg ctgctgggtg tgcacggcgt gctgcgcgct tcgcagggac aggtcctgcg
    17821 cgggttcgcg cagctcgcca cggccgtcgg cctcggggcg ctcgccaaag gtccggtcat
    17881 cctgctgcat ctgctgccgg tcgcgttgct cgcgccctgg tggatggcac agccgcgccc
    17941 gccggccgca cgctggtacc tgggggtcgt cgccgccgcg ctgctcggcg gcgggatcgt
    18001 gctgagctgg gcactgccgg cggggatcgc cggtggatcg gcctaccgcg acgcgatcct
    18061 gtggggccag accgccgggc gcatggcgca ctcgttcgcg cacccgcacc cctggtggtg
    18121 gtacctgccc atgctaccgg cgatcctgtt cccgtggccg ctgtggcccc cgctgtggcg
    18181 ggcgctcaac cggctgcgca ctgcgccgtc cgatccaggc gtgcggttgc tgctcgcctg
    18241 gattgtgccc ccgttcatcg gcttctcgct gatcagcggc aagcagccga actacttgct
    18301 gccgctggtt cccggggtcg cgctgctcgc cgcgcatgct ctgcatgcct tcgcgcgggt
    18361 cgatctgctg aatcgggtgc gactgctcgc ggcggcaagt ccggcgctgg tgttggcgat
    18421 caatgccgga atcatccgga atgtaggcgt ggatcacgat ctgcacccga tcggcgatca
    18481 tctacgcgca ctgcagcagt ccggtgtccc gatcgcgcat gtcgggagct acctgggaca
    18541 gttccacttc gtcgggcgac tcacgcggcc gatcgaggtg gtggagcccg aacaggtgca
    18601 gcgctgggtg gaaggccacc cctgcggtcg cctcatcggt tacagagacg ccgcgccggc
    18661 gcgcgcagca cgaggaagcg tgccgggctc gccctcgacg cgcagctacc cgctcggcga
    18721 gcccgagatc atccagggct atcgccggcg tcaggtcttc gtctggcgtc ccgggccgat
    18781 ctgcgccgcg gatgcggtgc gctgggcgcg tcgtccgcaa cccgcgctca tccgccgctc
    18841 gcgccctgcg tattgacgta gagcgcatac acgctcttgc tcgccgccat gaacaggcgg
    18901 ttgcgatgca ggcccccgaa gcacaggttg gcgcagcgct ccggcagatc gatgcgcccg
    18961 atcagccggc cgtcgggatt gaacacggcg accccgtcga gcgcggcttc gcccatcccc
    19021 caccccatcc acaggttgcc gtccgcgtcg cagcgcatgc cgtcgggcgt gcccgggccg
    19081 acgtcgatca gcttgcgccg gttggcgacg cgccggccgc cgtccaccac gtcgtaggcg
    19141 tagacgttgc gcggcgaggt gcccgactcg accagataca gaatcgattc gtccggcgag
    19201 aagcacaggc cgttggggcg attcacatcg gactcgacga cggtcatctc gccgcgcgcg
    19261 tccacccggt acacgttcat cggcagctcc tgcttcgccg ggtaaccctc gtaccagccg
    19321 aggaggccga acggcggatc ggtgaaccac accgagccgt cggacttcac caccacgtcg
    19381 ttcggcgagt tgagcggctt gccctcgaag ctgtccgcca ccgccgtgac ggtgccgtcg
    19441 tactcggtgc gcgagacgcg gcgcccgccg tgctcgcagg tgatcaggcg gccttgccgg
    19501 tcgcgggtgt tgccgttggc gtggttcgac ggcttgcgga aaacgccgac ggccccggtt
    19561 tcctcctccc acttcatgat gcggttgttg gggatgtcgc tccacaggag aaagcgcccg
    19621 tcgccaaacc acaccggccc ttccgcccag cggaaaccgt gcgcgatgcg ctccacggcc
    19681 gcgagattga gccggtacct ggcgaagctc tggtcgagca cgcgcacggc ggcgtcggga
    19741 tagcgctggc tcggttgcca gggcacctct ggaacttctg tctgcatcgt ctcctcctct
    19801 cgcctggatg gcaggcgcga cgactatact aaccacagag acacagaggc actctaaaag
    19861 gggttttccc tgtgtctgtg tgccgatgtg gtcaagaaac tacaagctca ggtgctcccg
    19921 cttcaccgcg tcgttcgccg ccagctcgcg catcgtcccg ctgtggcgga tctcgccctt
    19981 ctctatgacg taggcgcggt cgctcaccag ggccgcgaag tgcaggttct gctcgcacag
    20041 caggacgctg agcccctccc gcttcagctc cagaatcatg ttcgccatct cttcgacgat
    20101 tctcggagcg atgccttccg aaggctcgtc gagcagcacc aggagggggt tgcccatgag
    20161 cgtgcgcgcc accgtgagc
//
LOCUS       DASPGE010000145         3036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1564860, whole genome shotgun sequence.
ACCESSION   DASPGE010000145 DASPGE010000000
VERSION     DASPGE010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564860"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..>3036
                     /locus_tag="VD791_04590"
     CDS             368..>3036
                     /locus_tag="VD791_04590"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HXV07281.1"
                     /translation="MKLNDSILSLPPQQVSRDVLLEKYAKGGELTVEQVRRRVARALA
                     AVEKEPGRWEPVFFETLESGFIPGGRINSAAGTQLAATLINCFVQPVGDSVSETVDGK
                     PGIYVALMEAAETMRRGGGVGYDFSSIRPKGALVRGTQSSASGPVSYMRVFDQSCETV
                     ESAGARRGAQMGILRCDHPDIEEFIHAKDHGELRNFNVSVAVTDAFMRAVESDGDWEL
                     VHKQPPGPEYQGGVPAQRADGKWVFRTLKARDLWKQIMDSTYDHAEPGVVFIDAANRD
                     NNLSYCEVIEASNPCGEEMLPAYGCCCLGSINLTLFVAEPFTADSRFDFDAFAKVVRP
                     SIRMLDNVLDATVWPLPQQLEEARDKRRVGLGFTGLGDALIMLGLRYDTDAARRMAAQ
                     IAERMRDEAYAASVEIAKEKGAFPLFDKARYLSAPRFAARLPETLKGEIRRHGIRNSH
                     LLAIAPTGTISLAFADNAANGIEPPFSWTYQRKKRLADGGFKTYEVEDHAWRLYRHLG
                     GDMAKLPLQFVTALEISALDHMRMVAAVAPYIDSAISKTVNVAEDYPYEDFKDLYLEA
                     WRSGLKGITTYRPNKVLGAVLSVTPESQQPHDLDLSDVDRRIRLEAAPTPALYSLRWP
                     GRPQLPGGNPAWTYMVESPFGTFAIFVGHVENGRPHPFEVWVNGTEQPRGLGAVAKTL
                     SMDMRAQDKAWLRMKLDILARTAGDDAFDCPMPPKGKPTRVPSMVAAFARIVRWRVDQ
                     LGGLAKDEGSSPVLDALFTKKEPKTGTDGTMSWTVDVYNPSSGDDFLLILKELVLPNG
                     QRRPYSVWMAGAYPRALDGLCKLLSLDMRVIDPAWIGMKLRKLLNYAEPLGDFMARVP
                     GEERQQNFPSTVAYVARLMIHRYAMLGVLDERG"
BASE COUNT          511 a         1041 c         1041 g          443 t
ORIGIN      
        1 catcgtcgag agaaatcagc gcttcgtcgc aggaaggcga cggttggccg ccctcatccc
       61 tggaatccag cacggcgcag aggatttcgc gatagcggcc gcgcgcgtcc acggcgaagc
      121 ccggctcgaa tggggccgtg acgcaggccg acaaaacgag cgcgacgacg cacgccgcgg
      181 ccgaccgccg tcccgctgcc gctcccggat tcgctcgcgc acttgccgca tgtcccacga
      241 ttcgcatgat agcgcgcggc ggcgccgctc ctcgaccgga cgcgacgcgc ctggcgtcac
      301 gtcccggagc gggctcgccg cgtgccccag ccgggccaac atgcgacaat agcggccgag
      361 cgaagacatg aaactgaacg acagcatcct ctctctcccg ccgcagcagg tcagccggga
      421 cgtgctgctg gagaagtacg ccaagggcgg cgagctgacc gtcgagcagg tccgccggcg
      481 cgtggcgagg gcgctggccg cggtggagaa ggaaccgggc aggtgggagc cggtgttctt
      541 cgagacgctg gagagcggct tcatccccgg cgggcgcatc aactccgccg ccgggacgca
      601 gctcgcggcc acgctgatca attgcttcgt ccagccggtg ggcgattcgg tgtccgagac
      661 cgtggacggc aagccgggca tctacgtggc gctgatggag gccgccgaga ccatgcgccg
      721 cggcggcggc gtgggctacg acttctcctc gatccgcccg aagggtgcgc tggtgcgcgg
      781 cacgcagagc agcgcgagcg gcccggtgtc ctacatgcgc gtgttcgacc agagctgcga
      841 gacggtggaa tcggccggcg cccggcgcgg tgcgcagatg ggcatcctgc gctgcgacca
      901 tcccgacatc gaggagttca tccacgccaa ggaccacggc gagctgcgca acttcaacgt
      961 cagcgtcgcg gtcaccgacg ccttcatgcg cgcggtggag agcgacggcg actgggagct
     1021 ggtgcacaag cagccgccgg gccccgagta ccagggtggt gtgcccgcgc agcgcgcgga
     1081 cggcaagtgg gtctttcgca cgctgaaggc gcgcgatctg tggaagcaga tcatggactc
     1141 cacctacgat cacgccgagc cgggcgtggt cttcatcgac gccgccaacc gcgacaacaa
     1201 cctttcctac tgcgaagtca tcgaggctag caatccgtgc ggcgaggaaa tgttgccggc
     1261 ctacggctgc tgctgcctgg gcagcatcaa tctgacgctg ttcgtcgcgg agccgttcac
     1321 cgccgactcg cgtttcgact tcgacgcatt cgccaaggtg gtgcgcccgt cgatccgcat
     1381 gctcgacaac gtgctcgacg cgaccgtgtg gccgctgccg cagcagctcg aggaggcgcg
     1441 cgacaagcgg cgcgtggggc tgggcttcac cggcctgggc gacgcgctga tcatgctcgg
     1501 cctgcgctac gacaccgacg cggcgcgccg catggcggcg cagatcgccg agcgcatgcg
     1561 tgacgaggcg tacgccgcct ccgtggaaat cgccaaggag aaaggcgcgt tcccgctgtt
     1621 cgacaaggcg cggtacctga gcgcgccgcg cttcgccgca cgcctgcccg agacgctgaa
     1681 gggcgagatc cgccggcacg gcatccgtaa cagtcatctg ctggcaatcg cgcccaccgg
     1741 caccatctcg ctcgctttcg ccgacaacgc cgccaacggc atcgaaccgc cgttctcctg
     1801 gacctatcag cgcaagaagc gcttggccga cggcggcttc aagacctacg aggtggagga
     1861 ccacgcctgg cgcctgtacc ggcacctggg cggggacatg gccaagctgc cgttacagtt
     1921 cgtcaccgcg ctggagatct cggcgctcga tcacatgcgc atggtggcgg cggtcgcgcc
     1981 atacatcgac tcggcgatca gcaagacggt gaacgtggcc gaggactatc cgtacgagga
     2041 tttcaaggac ctgtacctgg aggcctggcg ctcgggcctg aagggcatca ccacctaccg
     2101 gcccaacaag gtgctgggcg ccgtgctgtc ggtgacgccc gaatcccagc agccgcacga
     2161 cctcgatctg agcgacgtcg atcgccgcat ccggctggaa gccgcgccga cgcccgcgct
     2221 gtacagcctg cgctggcccg ggcgcccgca actacccggt ggcaacccgg cgtggaccta
     2281 catggtcgaa tccccgttcg gcaccttcgc gatcttcgtg gggcacgtcg agaacggccg
     2341 accgcatccc ttcgaagtgt gggtcaacgg caccgagcag ccgcgcggcc tgggcgcggt
     2401 ggccaagacg ctgtccatgg acatgcgcgc gcaggacaag gcgtggctgc gcatgaagct
     2461 ggacatcctg gcgcgcaccg ccggcgacga cgcgttcgac tgcccgatgc cgcccaaggg
     2521 caagccgacg cgcgtgccga gcatggtggc cgccttcgcc cgcatcgtgc gctggcgcgt
     2581 ggaccagctc ggcgggctgg cgaaggacga gggttcctcg ccggtgctcg atgcgctgtt
     2641 caccaagaag gagcccaaga ccggcaccga cggcaccatg tcctggaccg tggacgtgta
     2701 caaccccagc tcgggcgacg acttcttgct gatcctgaag gaactggtgc tgcccaacgg
     2761 ccagcgccgg ccttactcgg tgtggatggc gggcgcctac ccgcgcgcgc tggacggcct
     2821 atgcaagctg ctgtcgctgg acatgcgcgt gatcgatccg gcatggatcg gcatgaagct
     2881 gcgcaaactg ctcaactacg ccgagccgct gggcgacttc atggcgcgcg tgccgggcga
     2941 ggagcggcag cagaacttcc cgtccacggt ggcctacgtc gcgcgcctga tgatccatcg
     3001 ctacgccatg ctcggcgtgc tcgacgagcg cggtta
//
LOCUS       DASPGE010000146         6936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1214098, whole genome shotgun sequence.
ACCESSION   DASPGE010000146 DASPGE010000000
VERSION     DASPGE010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214098"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VD791_04595"
     CDS             complement(<1..1038)
                     /locus_tag="VD791_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HXV07282.1"
                     /translation="MLLCVLTAPAVRADELVVGSKRFTESYILGEIAAQTVRAAGEGS
                     VTHAQGLGNTAILFEALKSGSIHLYPDYTGTVGRELLKSEATDLDALNRGLAPLGLGV
                     AVRLGFNNTYALAALDTLAVERGLRGIGDLAGHPDLRFGLSHEFLNRNDGWPGLKRAY
                     ALPHRPGGLDHGLAYEAIAAGQIDVMDIYSTDAKIERYKLRVLADDKAYFPEYQALFV
                     YRLDVPQRFPKAWAALARLEGAIDEPRMIAMNAAAELDAKPFSAVAAEFLAGEGAKPV
                     ATRQGLAARLFGPDLWKLTAQHLMLVFLSLAFAVAVGVPLGIWAARRPGAAQPILSAV
                     GLIQTVPSLALL"
     gene            complement(1110..1853)
                     /locus_tag="VD791_04600"
     CDS             complement(1110..1853)
                     /locus_tag="VD791_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV07283.1"
                     /translation="MLAVHNVSKSFDGTLALAPTSLEFAAGRTTVLLGPSGCGKTTLL
                     KLLLGLLVPDGGEVRFHGAPVTPARVDALRHRAGYVIQEGGLFPHLSARDNVCLMARH
                     LGRDSAWIERRITELSDLARLPAESLSRFPGELSGGQRQRVALMRGLMLDPDVLLLDE
                     PLGALDPMTRHQLQLDLKAIFGRLARTVVLVTHDMHEAAWFADEIVLMRAGRVVQRGA
                     LEDLLQRPAEGFVTEFVRAQRADPRLAAG"
     gene            complement(1888..3255)
                     /locus_tag="VD791_04605"
     CDS             complement(1888..3255)
                     /locus_tag="VD791_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXV07284.1"
                     /translation="MPHNVFDSILGTVGNTPLVRLNRIARGVKATVYAKVETFNPGHS
                     IKDRMALRMIEEAERQGLLKPGGTIIEGTSGNTGMGLAIAAIVKGSKCIFTTTDKQSK
                     EKVDALRAFGAEVIVCPTDVDPEDPRSYYSVSSRLVAETPNSWKANQYDNLSNSQAHY
                     ETTGPEIWEQTQGGIDHLVVGVGTGGTVCGVGKFLKERKPSVKVWGIDTYGSVFKKYK
                     ETGVFDKHEIYPYVTEGIGEDFLPRNVDFSVIDHFEKVTDRDAAIYTREIVRQEGIWA
                     GNSAGSAIAGLLQLKDNFRAGETVVVIFHDHGSRYLGKMFNPDWMRTMGYETLPGRTA
                     RDLVKNKKVTDIVGVKVTDSVEYAIQRMHADDFSQIPVTQEQRIVGSLSESHAYACIV
                     KDPRIRTEAVRNIMQKAFRFVDIDTPVQLLAPMITPENPAVLVRDFKAGKTYILTGYD
                     VLNAI"
     gene            complement(3327..4373)
                     /gene="selD"
                     /locus_tag="VD791_04610"
     CDS             complement(3327..4373)
                     /gene="selD"
                     /locus_tag="VD791_04610"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HXV07285.1"
                     /translation="MNAPEVPRLTSLSHGGGCGCKIAPGVLEQIVAKTASGFVPKELL
                     VGIETSDDAAVYRIGDDQAIVATTDFFMPIVDDPFDFGAIAATNAISDVYAMGGTPLF
                     ALALVGMPINVLPLETIHRILEGGESVCRTAGIPIAGGHSIDSVEPIYGLVAIGLVNP
                     RHMRRNADAKAGDKIVLGKPLGVGVYSAALKKDALSQAHYREMLASTTKLNTPGAKLG
                     RIEGVHALTDVTGFGLLGHLLEVCRGSGLAARLHWDKVPLLPGVLDLVTANHRTGASS
                     RNWAGYGTRVDLGRHGDVQRDLLTDPQTSGGLLVTCAPAAADAVLDLFRAEGFARAAV
                     IGEMTAGEPWVSVL"
     gene            complement(4486..5469)
                     /gene="senB"
                     /locus_tag="VD791_04615"
     CDS             complement(4486..5469)
                     /gene="senB"
                     /locus_tag="VD791_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine biosynthesis selenosugar synthase
                     SenB"
                     /protein_id="HXV07286.1"
                     /translation="MPLPVVRIVTPYGAAANNGNWRTAARWARLLRGHYRTMVQADTL
                     AGEAPEDSRAVCLIALHARRSYPAIRAWRERYPRRPLIVVLTGTDLYRDLPCDAQAQA
                     SLRLADRLVLLQEDALRHLPAQVRRKARVIHQSASTLTPAERPVSRLNCILVGHLRAE
                     KDPGTVLRAWSLLAPELPLHLLHVGGALDAGLAAECEASMARDPRYRWVGARPHAWTR
                     QAIKRAHLLILPSLMEGGANVIVEAVTAGTAVLASRVSGNVGMLGHGYAGYFPAGDAQ
                     ALARLVQRCWSDAAFHRRLARECRARRALFTPAHERAALLRVLSEMLPDYS"
     gene            5474..6772
                     /gene="senA"
                     /locus_tag="VD791_04620"
     CDS             5474..6772
                     /gene="senA"
                     /locus_tag="VD791_04620"
                     /inference="COORDINATES: protein motif:HMM:NF041186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1903257 - selenoneine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine synthase SenA"
                     /protein_id="HXV07287.1"
                     /translation="MRELNDVDALRAALIDARDYTLALYAHLSPGQLRFPYLRIVNPP
                     LWELAHIGWFQEHWCLRWRGGNAMPPARLADADSILNSALIPHAARWELPQLTRDTVH
                     AYLERELRDTLAALDSAPPDRLYFFLLALFHEDMHGEALLMTLQTLGYPAPQLNRPVL
                     PRPAPVPTARGAEIEFAGGRVHIGATPGADFVFDNEKWAHEVDLEPFALSATQVSNAE
                     FAAFVEAGGYEDRRLWSDAGWTWRTEHDAHRPRYWQREDGAWLTRRFDRWEALAPDQP
                     VLHVNAYEAEAYCSFVGRRLPTEAEWEYAARSGSAEGADRYPWGDAPARRGSVNLGGE
                     FDGPVPVAGLPDADTRTGLRQMLGNVWEWTSSAFAPYPGFAPDPYQEYSQPWFHDHRV
                     LRGGCFATRARLVHNRWRNFYLPERNDIFAGLRTARTLPA"
BASE COUNT         1044 a         2400 c         2413 g         1079 t
ORIGIN      
        1 gagcagcgcg agcgacggca ccgtctggat caggccgacc gcgctcagga tcggttgcgc
       61 cgcgcccggc cggcgcgcgg cccagatgcc gagcggcacg ccgaccgcga cggcgaacgc
      121 gagcgacaag aagaccagca tcaggtgctg ggcggtcagc ttccatagat cgggcccgaa
      181 cagccgcgcc gccagccctt gccgcgtcgc gaccggcttg gccccctccc cggccaggaa
      241 ctcggcggcc accgcgctga acggcttcgc atcgagctcc gcggccgcgt tcatcgcgat
      301 catgcgcggc tcgtcgatcg cgccctcgag cctggcgagc gccgcccagg ctttcgggaa
      361 gcgctgcggg acgtccaggc ggtagacgaa cagcgcctgg tactcgggaa agtacgcctt
      421 gtcgtcggcg agcacgcgca gcttgtagcg ttcgatcttg gcgtcggtgg aatagatgtc
      481 catcacgtcg atctggccgg cggcgatcgc ttcgtaggcg aggccgtgat cgagaccgcc
      541 gggccggtgc gggagcgcat acgcgcgctt cagacctggc cagccgtcgt tgcggttgag
      601 gaactcgtgc gacaggccga agcgcaggtc gggatggccc gccagatcgc cgatgccgcg
      661 caggccgcgc tcgacggcaa gcgtatcgag cgccgccagc gcgtaggtgt tgttgaagcc
      721 gaggcgcacc gccacgccga gcccgagggg cgcaagcccg cgattgagcg cgtccagatc
      781 ggtggcctcg gacttcagca gctcgcgacc cacggtgccg gtgtagtcgg ggtacagatg
      841 gatgctgccg ctcttgagcg cctcgaacag gatcgcggtg ttgcccaggc cctgcgcgtg
      901 cgtcacgctg ccctcgcccg ccgcgcgcac cgtctgtgcg gcgatctcgc ccaggatgta
      961 ggactcggtg aagcgcttcg agccgaccac cagctcgtcg gcgcgaacgg cgggagccgt
     1021 gagcacgcag agcagcatga gccccgaggt ttgcgccgct gtgcagcacg cgcggcgcct
     1081 cgcccctcgt gcctcgtgcc tcgcgcccat catcccgccg ccagcctcgg gtccgcgcgc
     1141 tgggcgcgga cgaactcggt gacgaagcct tccgcgggcc gctgcaacag gtcttcgagc
     1201 gcgccacgct gcaccacccg gcccgcacgc atcagcacga tctcgtccgc gaaccacgcg
     1261 gcctcgtgca tgtcgtgcgt caccagcacc acggtgcgcg caagccgccc gaaaatcgcc
     1321 ttgagatcga gctgaagctg gtggcgcgtc atgggatcca gcgcgcccag cggctcgtcg
     1381 agcagcagca cgtccgggtc gagcatcagc ccacgcatca gcgccacccg ctggcgctgc
     1441 ccgcccgaca gctccccggg gaagcgcgac agcgattcgg cgggcagccg cgccaggtcg
     1501 ctcagctcgg tgatgcgacg ctcgatccag gcgctgtcgc ggcccaggtg ccgcgccatg
     1561 aggcaaacat tgtcgcgcgc gctgaggtgc ggaaacagcc cgccctcctg gatcacgtag
     1621 ccggcccggt ggcgcagcgc gtcgacgcgc gcgggcgtga ccggcgcgcc gtggaagcgg
     1681 acttcgccgc cgtcggggac gagcaggccg agcagcagct tgagcagcgt ggtcttgccg
     1741 cagccgctgg ggccgagcag gaccgtggtg cggccggcgg cgaattcgag cgaggtcggc
     1801 gccagggcga gcgtgccgtc gaaagacttg gagacgttgt ggacggcgag cacgcgaacg
     1861 ccgctttgct gtggatcggg gactgcgcta gatcgcgttc aacacgtcgt agccggtgag
     1921 gatgtaggtc ttgccggcct tgaagtcgcg caccagcacc gccgggttct ccggcgtgat
     1981 catcggcgcc aggagctgca ccggcgtgtc gatgtccacg aagcggaacg ccttctgcat
     2041 gatgttccgc accgcctcgg tgcggatcct cgggtccttg acgatgcagg cgtaggcgtg
     2101 cgattcgctg agcgagccga cgatgcgctg ctcctgcgtc accgggatct gcgagaaatc
     2161 gtccgcatgc atgcgctgga tcgcgtactc caccgaatcg gtgaccttca ctcccacgat
     2221 gtcggtgacc ttcttgttct tcaccaggtc gcgcgccgtg cggccgggca gggtctcgta
     2281 gcccatggtg cgcatccagt cgggattgaa catcttgccc aggtagcgtg acccgtgatc
     2341 gtggaagatc accaccacgg tttcgcccgc ccggaagttg tccttgagct gcagcaggcc
     2401 ggcgatggcg gagccggcgg agttgcccgc ccagatgccc tcctgacgca cgatctcgcg
     2461 cgtgtagatg gcggcgtcgc ggtcggtcac cttctcgaag tggtcgatca cggagaagtc
     2521 cacgttgcgc ggcaggaaat cctcgccgat gccctcggtg acgtacgggt agatctcgtg
     2581 cttgtcgaag acgccggtct ccttgtactt cttgaacacg gagccgtaag tgtctatgcc
     2641 ccagaccttg accgagggtt tgcgctcctt gaggaacttc ccgacgccgc agacggtgcc
     2701 gccggtgcct accccgacca ccaggtggtc gatcccgccc tgcgtctgct cccagatctc
     2761 cgggccggtg gtctcgtaat gcgcctggct gttgctcaga ttgtcgtact ggttcgcctt
     2821 ccacgagttg ggcgtttcgg ccaccaggcg cgaagacacc gagtagtagg agcgcgggtc
     2881 ctcgggatcg acgtcggtcg ggcacacgat cacttcggcg ccgaaggcgc gcagcgcatc
     2941 caccttctcc ttggactgct tgtcggtggt ggtgaagatg catttggagc ccttgacgat
     3001 ggcggcgatc gccaggccca tgccggtgtt gccgctggtg ccttcgatga tggtgccacc
     3061 cggcttgagc aggccctggc gctcggcctc ctcgatcatg cgcaaggcca tgcggtcctt
     3121 gatgctgtgg ccggggttga aggtttcgac cttggcgtac acggtggcct tcaccccgcg
     3181 cgcgatgcgg ttcaagcgca ccagcggcgt gttgccgacg gtgccgagga tgctgtcgaa
     3241 gacattgtgc ggcatcgccg gccggccgtc cgtttccacg cgcgcgaccg ggcgcggttc
     3301 cgatgcggtg gaacgggctc ttcccatcag agcacgctca cccacggctc gccggccgtc
     3361 atctcgccga tcaccgccgc gcgcgcgaac ccctccgcgc ggaacaagtc cagcaccgcg
     3421 tctgccgccg cgggggcgca ggtcaccagc agcccgccgc tggtctgcgg atcggtgagc
     3481 agatcgcgct gcacgtcgcc gtggcggccg aggtccacgc gggtgccgta gccagcccag
     3541 ttgcgcgacg aggcgccggt gcggtggttg gccgtcacca ggtccagcac cccgggcagc
     3601 agcggcacct tgtcccaatg caggcgcgcg gcgaggccgg agccgcggca cacctccagc
     3661 agatggccga gcaggccgaa cccggtgacg tcggtcaggg cgtgcacacc ctcgatgcgg
     3721 cccagcttcg cgccgggcgt attgagcttt gtcgtgctgg cgagcatctc gcggtagtgc
     3781 gcctgcgaga gcgcgtcctt cttcaacgcc gcgctgtaga cgcccactcc cagcggcttg
     3841 cccagcacga tcttgtcgcc ggcctttgcg tccgcgttgc ggcgcatgtg ccgtggattc
     3901 accaggccga tggcgaccag cccgtagatc ggttccaccg aatcgatgct gtgcccgccc
     3961 gcgatcggga tgcccgcggt gcggcacacc gactcgccgc cctccaggat gcggtggatg
     4021 gtctccagtg gcagcacgtt gatcggcatg cccaccagcg ccagcgcgaa caacggcgtg
     4081 ccgcccatgg cgtacacgtc agaaatcgcg ttggtggcgg cgatcgctcc gaaatcgaac
     4141 gggtcgtcga cgatgggcat gaagaaatcg gtggtcgcga cgatcgcctg gtcatcgccg
     4201 atgcggtaca ccgcggcgtc gtcgctggtc tcgatgccca ccagcagctc cttgggcacg
     4261 aagcccgagg cggtcttggc gacgatctgc tccagcacgc cgggggcgat cttgcagccg
     4321 cagccgccgc cgtgggacag gctggtgagg cgcgggacct cgggggcatt catgcgtgtt
     4381 ctcctgctgt gcggcggtca ttttatgcca caaacggaac gaaggagccg tgcttgcccc
     4441 ggattccgct gcgctgcatc cgggctacgg gcgactgcat ccgggctacg agtaatcagg
     4501 gagcatttcg ctcagcacgc gcagcaaagc cgcacgctcg tgcgccggcg tgaacagcgc
     4561 gcgccgcgcg cggcattcgc gcgcgaggcg ccgatggaac gcagcgtcgc tccagcaacg
     4621 ctgcaccagc cgcgccagcg cctgcgcgtc ccccgccggg aaatagcctg cgtagccgtg
     4681 gcccagcatg ccgacgttgc ccgacacccg gctggcgagg accgccgtgc cggcggtcac
     4741 cgcctcgacg atgacgttcg cgccgccttc catgagcgag ggcaggatca gcagatgggc
     4801 gcgctttatc gcctgccgcg tccaggcgtg gggccgtgcg cccacccagc ggtagcgggg
     4861 atcccgtgcc atcgaggctt cgcactcggc ggcgaggccg gcgtcgagcg ctccgcctac
     4921 atgcaggagg tgcagtggca gctcgggcgc caacagcgac caagcccgga ggacggtccc
     4981 gggatccttc tccgcgcgca ggtgcccgac gaggatgcag ttcagccgcg agaccggccg
     5041 ctcggcgggc gtgagcgtgg acgccgattg atggatcacg cgcgccttgc gccggacctg
     5101 cgccggcaga tggcgcagcg catcctcctg caggagcacc aggcggtcgg caagccgcag
     5161 cgacgcctgc gcctgcgcgt cgcacggcag gtcgcggtag agatcggtac cggtgagcac
     5221 cacgatcagc ggccgccgcg gataccgctc gcgccaggcg cgaatggccg gatagctgcg
     5281 gcgcgcatgc agcgcgatca ggcaaacggc gcgtgagtcc tcgggcgctt cccccgcaag
     5341 cgtgtccgcc tgtaccatgg tccggtagtg tccacgcagc aggcgcgccc agcgcgcggc
     5401 ggtgcgccag ttgccgttgt tggcggcggc gccgtagggt gtgacgatcc ggacgacagg
     5461 aaggggcacg acagtgcggg agctcaatga cgtcgatgcg ttgcgcgcgg ccctgatcga
     5521 cgcgcgcgat tatacgctcg ccctgtatgc ccacctctct ccgggccagc tgcgcttccc
     5581 gtatctgcgc atcgtcaatc cgccgctgtg ggagctggcg cacatcggct ggttccagga
     5641 gcactggtgc ctgcgctggc gggggggcaa cgccatgccg cctgcgcggc tggcggacgc
     5701 ggactcgatc ctgaattcgg cgttgatccc gcatgccgcc cgctgggagc tcccgcaact
     5761 gacacgtgac acggtgcacg cctatctcga acgcgagttg cgggacacgc tcgcagcgct
     5821 cgacagcgca ccgcccgatc gcctgtattt cttcctgctc gcgttgtttc acgaggacat
     5881 gcacggcgag gcgctgctga tgacgctgca gacgctgggc tatcccgcgc cgcagctgaa
     5941 ccgcccggtg ttgccgcggc cggcgcccgt accgacggcg cgcggcgcag agatcgagtt
     6001 cgccggcggg cgcgtgcaca tcggcgcaac gcccggtgcg gatttcgtgt tcgacaacga
     6061 gaagtgggcg cacgaggtcg atctcgagcc gttcgcgttg tccgcgacgc aggtcagcaa
     6121 cgccgagttc gccgcgttcg tcgaggccgg cggttacgag gatcggcgct tgtggtccga
     6181 cgcgggatgg acctggcgca ccgagcacga cgcgcatcgc ccgcgctact ggcagcgcga
     6241 ggacggcgcc tggctcacgc gccgcttcga ccgctgggaa gcgctggctc cagaccaacc
     6301 ggtgctgcac gtgaatgcgt acgaggccga ggcctattgc agcttcgtcg gccgccggct
     6361 gcctaccgaa gcggagtggg agtacgccgc gcgcagcgga tccgcggagg gcgccgaccg
     6421 ctatccctgg ggagacgcgc cggcacgtcg cggcagcgtc aatctcggcg gcgagttcga
     6481 cggaccggta cccgtggccg gtctgcccga tgccgacacg cgcacggggc tgcgtcagat
     6541 gctgggcaat gtgtgggagt ggacctccag cgccttcgcc ccctaccccg gcttcgcacc
     6601 cgacccctac caggaatact cgcagccgtg gttccacgac catcgcgtgc tgcgcggcgg
     6661 ctgcttcgcc acgcgcgcgc gcctggtgca caaccggtgg cgcaatttct atcttccgga
     6721 gcgcaacgac atcttcgcgg gcctgcgcac ggcacgcacg ctgcccgcct gaggtcgcgc
     6781 ccgtctcagt ccttgagcag cgccggcggc gaatcgcgcg cgatccactc ctgcagcagc
     6841 cggaagccga cggcgagcaa cgtcgggccg agaaacacgc cgacgatgcc gaaggcgagc
     6901 gcgccgccga gcacgcccag cagcgtcagg atgaaa
//
LOCUS       DASPGE010000147         2538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_862886, whole genome shotgun sequence.
ACCESSION   DASPGE010000147 DASPGE010000000
VERSION     DASPGE010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862886"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VD791_04625"
     CDS             complement(<1..328)
                     /locus_tag="VD791_04625"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXV07288.1"
                     /translation="MRAPADDRRVYLTFDDGPDPGWTPRILDVLAAANASATFFVVGR
                     IARQAAAVIRRLADAGHEIENHSWSHRHPWTLPRAAARAEVRDGAAAVADLVGRKPSY
                     FRPPHGR"
     gene            complement(312..812)
                     /locus_tag="VD791_04630"
     CDS             complement(312..812)
                     /locus_tag="VD791_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07289.1"
                     /translation="MLLIGRDRHDGPARRFPDGTEIGPGTPIGTLHFDNARIAALDAE
                     TPNAIGLSFTRLLLESLANLAELAGADGPYGDIAVFRGIGWTRQGERLGFVSEPFPQG
                     RRKRFLTVHIGLLVWAFAPARGTAIAARPEPRLIWLTREALLQHFGNRRKHARQATAA
                     ADARAG"
     gene            1101..2057
                     /locus_tag="VD791_04635"
     CDS             1101..2057
                     /locus_tag="VD791_04635"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HXV07290.1"
                     /translation="MKIAVLGCGAMGSVYAALLAAAGHEVWAIDSWREHVQAMRSRGL
                     RLEGASGDRTVAVHATTDPAEAGDCDLVIIATKAMHVEQAARSAGPLIGPNTLVLSIQ
                     NGLGGPDTAAKVLGRDRVLVGVVGGFGASMRGPGHAHHNGWELVRLGEFGGPATPRLE
                     RIAEVWRSGGFRVKCFDDIDQLVWEKLICNVCFSGTCAITERTIIEVVEDADTWECAS
                     GCAREAFAVAKARGIRVEIDDPVRYVRDFGMKIPHARPSMLLDHMAGRMSEIDAINGA
                     IPVAALAVGVDAPYNTVISALVRAKERRMGVRPPAPGKDSSS"
     gene            2070..>2538
                     /locus_tag="VD791_04640"
     CDS             2070..>2538
                     /locus_tag="VD791_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXV07291.1"
                     /translation="MEKYRLYIDGKFVDAVAGKWFDSYNPYTGKVWAQVAEGGAEDVD
                     RAVKAAHRAFTEGPWPQLTASQRGLLLHRLGELVARDAKRLAEIEVLDNGKLIAEMQA
                     QLNYVPQWYYYYGGLADKIQGAVIPLDKKGYFNFTRHEPLGVIAAITAWNSPLL"
BASE COUNT          403 a          857 c          916 g          362 t
ORIGIN      
        1 gcctgccgtg cgggggacga aagtagctcg gcttgcggcc gaccaggtcg gcgaccgcgg
       61 ccgcaccgtc gcgcacttcc gcccgcgccg cggcgcgcgg caaggtccac gggtggcggt
      121 ggctccagct gtgattctcg atctcgtgtc cggcatcggc gagacgtcgg atcacggcgg
      181 cggcctgacg cgcgatgcgc cccacgacga agaacgtggc gctggcgttg gcggccgcga
      241 gcacgtcgag aatccgcggt gtccacccgg ggtcgggtcc gtcgtcgaac gtcaggtaga
      301 cgcgccggtc gtcagccggc gcgcgcatca gcggcggcgg tggcctgccg tgcatgtttc
      361 ctccgattgc cgaagtgctg cagcagcgcc tcacgcgtca gccagatcag ccgcggctcg
      421 ggccgcgcgg cgatggccgt gccgcgggcg ggcgcgaacg cccagaccag gagcccgatg
      481 tgcaccgtca ggaagcgttt gcgccggccc tgaggaaacg gctcgctcac gaatccgagg
      541 cgctcgccct ggcgcgtcca gccgatgccc cggaacacgg cgatatcgcc gtacggccca
      601 tccgcgcctg ccagctccgc caggtttgcg agcgactcga gcaacagccg ggtgaagctc
      661 aacccgatcg cgttcggcgt ttcggcgtcg agagccgcga tgcgtgcgtt gtcgaagtga
      721 agcgtcccga tcggggtccc gggtccgatc tctgtgccgt ccgggaatcg ccgcgccggc
      781 ccgtcgtgac ggtcccgccc gatgagcagc accggcccga ccggccgcag cccgtgccgc
      841 aggcgataca gcgcatcgac tctgcgcagg cagcttcgcg ccatgcagcg aaccgcttcc
      901 atcacggaat cctcgactcc tgacccctca cccccgcccc tctcccgcaa ggccccgcaa
      961 ggggagaggg gagtatcgga tcgatgatcg cacgatcgcg ccgatcgcgc agatggtcca
     1021 cgctgccccg acagacgacc ggcatgccgc aaccaaggca tactgatgcc tgatccggct
     1081 ccgggagggg gaacagcggg atgaagatcg cagtgctggg ctgcggcgcg atgggcagcg
     1141 tgtacgcagc cctgctcgcc gcggcgggtc acgaggtgtg ggccatcgat tcctggcgcg
     1201 agcatgtgca ggcgatgcgc agccgggggc tccggctgga aggcgcatcg ggcgaccgga
     1261 ccgtcgcggt gcacgccacc accgatccgg ccgaagccgg tgactgcgac ctggtcatca
     1321 tcgccaccaa ggcgatgcac gtggagcagg cggcgcgctc ggcgggcccg ctgatcggac
     1381 ccaacacgct ggtgctgtcg atccagaatg ggctgggcgg gccggacacg gcggccaagg
     1441 tgctggggcg cgaccgcgtc ctggtcgggg tggtcggcgg cttcggcgcc tccatgcgcg
     1501 gccccggcca cgcccaccac aacggctggg agctggtgcg gctgggcgaa ttcggcggcc
     1561 cggcgacgcc gcggctggag aggatcgccg aggtgtggcg ttcgggcggc ttccgcgtga
     1621 agtgcttcga cgacatcgat cagctcgtgt gggagaagct gatctgcaac gtctgcttct
     1681 cgggcacctg tgcgatcacc gagcgtacta tcatcgaggt ggtcgaggac gccgacacct
     1741 gggagtgcgc ctcgggctgc gcccgggaag ctttcgcggt ggccaaggcg cgcggcatcc
     1801 gggtggagat cgacgatccg gtgcgttatg tccgcgactt cggcatgaag atcccgcatg
     1861 cgcgcccgtc catgctgctc gaccacatgg cggggcgaat gtccgagatc gacgcgatca
     1921 acggcgccat cccggtggcc gcgcttgcgg tcggcgtgga cgcgccgtac aacacagtga
     1981 tcagtgcgct ggtgcgggcc aaggagcggc gcatgggtgt gcgtccgccg gcaccgggca
     2041 aggacagcag cagctaggaa ggagtactcg tggaaaagta tcggctgtac atcgacggca
     2101 agttcgtcga cgccgtggca ggcaagtggt tcgacagcta caacccctac accggcaagg
     2161 tgtgggcgca ggtcgccgaa ggcggcgcgg aggacgtgga ccgcgcggtc aaggccgcac
     2221 accgcgcctt caccgaaggg ccctggccgc agctcaccgc gagccagcgc ggcctgctgc
     2281 tgcaccgcct gggcgagctg gtggcgcgcg acgccaagcg gctggccgag atcgaggtgc
     2341 tcgacaacgg caagctgatc gccgagatgc aggcccagct caactacgtc ccgcagtggt
     2401 actactacta cggcggactg gccgacaaga tccagggcgc ggtgattccg ctggacaaga
     2461 agggttattt caacttcacg cgccacgagc cgctcggcgt gatcgccgcg atcaccgcct
     2521 ggaactcgcc gctgctgc
//
LOCUS       DASPGE010000148         6095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_159860, whole genome shotgun sequence.
ACCESSION   DASPGE010000148 DASPGE010000000
VERSION     DASPGE010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159860"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VD791_04645"
     CDS             <1..303
                     /locus_tag="VD791_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXV07292.1"
                     /translation="PWQKFWHVTLPMLSPIIAVVMTFSVLFTFTDFQLIWVLTRGGPI
                     NATHLMATLSFQRAIPGGALGEGAAIATAMIPFLLAAILFSYFGLQRRRWQQGMKD"
     gene            321..1190
                     /locus_tag="VD791_04650"
     CDS             321..1190
                     /locus_tag="VD791_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXV07293.1"
                     /translation="MKDQELGSGMRYLVSVPRRVVTVYVPLLVFLFVLLFPFYWMAVT
                     AIKPNAELLSTGGNPFWVIRPTLEHFEKLLLRTAYPSWMLNTMLIAVISTFASVAASV
                     LAAYAIERLRFRGSRQVGMAIFLAYLVPPSILFIPLATIVFQLGIFDTKWALILTYPT
                     FLIPFSTWLLMGYFRSIPFELEECALIDGASRWQILVKIVLPLAVPGLISAGIFAFTL
                     SWNEFLYALTFISSSEHKTVPVGVITELVEGDVYHWGALMAGALLGSLPVAILYSFFV
                     EYYVAGMTGAVKE"
     gene            1322..2398
                     /locus_tag="VD791_04655"
     CDS             1322..2398
                     /locus_tag="VD791_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HXV07294.1"
                     /translation="MSQPIRILHIGPAKSEAFDTSSLEQQSRAVRLAGGTVIRYEGRS
                     TAELVQALREVDAAMVQGHVFDQAAFAHMGLHGRCKGLASFGHGYDHLDLDAATANGV
                     VLANTASFGTEEVSNHTIMHFLVCARKFVLHDKLVRSGAWTRKNLAPMGHVAGQVFGI
                     VGLGDIGRAVARKAKAFGLQVIAYDPYVASWDALEYGVEMIDSLEALCRRSDYISVHV
                     YLNAETRHLIGRAQFAVMKPSAYLINCARGGVVDEAALIEALQQRRIAGAGLDVFEKE
                     PVASDNPLLAMDNVSLTNHYASYSETAWERAQTQLGEEAVRIATGMWPMSLINPDVRH
                     VIPPRAPARSWEVLARELAAAGHR"
     gene            2435..3343
                     /locus_tag="VD791_04660"
     CDS             2435..3343
                     /locus_tag="VD791_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXV07295.1"
                     /translation="MRINKALELLEQRQPVYFEFVDAEDAGEYDGGRALAQTWADYIA
                     YDMEHSALDVRGLMRFMRGLVDGGPTRSGHRTPAVIVTLPIDGTDEQSVRANGWMIKQ
                     VLATGVHGLLLCHAESPEAVRAFVEAARYAFHSSGGHAGAGHAGAGPELGPGRRGHGG
                     QTRAAEIWGVAPGEYLARADPWPLDARGELLLGLKVENKRALAAVERTTRVPGIAFAE
                     WGPGDMGMSMGYPDQHDEPYPAEMRAARARVMSACKVAGLAFLEAVTPENVIGRLEEG
                     VTIGCGPRAQEAADIGRRYTRRALPW"
     gene            3468..4784
                     /locus_tag="VD791_04665"
     CDS             3468..4784
                     /locus_tag="VD791_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXV07296.1"
                     /translation="MNRFTRRRFLTAGTVAAAAAAVPGLAPAQVAPLRFEPEKGATLR
                     VLRWKRFVQGDEDQFMANTRRFTQATGVQVRVDSENFEDLRPKAAVAANVGAGPDIII
                     STDEQPQLYPDKLLDVNDLAQYLGQKYGGWFEVCPEYCTYQGRWIAIPTGVAGGAMVY
                     RRSMLRAAGFEQFPKDLDGFLKLCRALKAKAAPPGFALAHATGDANGWTHWLLWAFGA
                     RQADEQSRLALNSKETVAALEYAKELYQTFAPGTLSWLDPSNNKAFLAGEIGLTMNGI
                     SIYYTAKTSADPKVKAMAADIEHAAFPIGPIGRDVHGALMFPGAIYKYCKYPNAAQEY
                     LRFIMEREQYEPWQRASIGYISHTLRAYDANPVWSEDPQHVFFRDIAKGARHTGYAGR
                     LGKESAAVLADFVIVDMFGEACTGRYAPKEALQRAEKRARRHYRRS"
     gene            complement(4779..5435)
                     /gene="adk"
                     /locus_tag="VD791_04670"
     CDS             complement(4779..5435)
                     /gene="adk"
                     /locus_tag="VD791_04670"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HXV07297.1"
                     /translation="MKLILLGAPGAGKGTQAVAICQHYGIPQISTGDMLHAAVRVGSP
                     AGLRIKKYMDAGQLVPDVVIMSLVKQRIAQPDCANGFLFDGFPRTITQTEAMEQAGVK
                     VDYVVELDVEDAEIIERLSGRRVHPGSGRSYHVRLRPPKVPDKDDLTGEDLIEREDDK
                     EEAVRKRLEIYHAETRHLVDYYSAWAASGDPRAPKYCKVSGSGSVEEIRDRVAAALTS
                     "
     gene            5657..>6095
                     /locus_tag="VD791_04675"
     CDS             5657..>6095
                     /locus_tag="VD791_04675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXV07298.1"
                     /translation="MEYDVVIVGAGPSGLAAAIRLKQLAAELSVCVLEKGSEVGAHIL
                     SGAVLEPRALDELLPDWKARGAPLDTPAREDRFLYLSAARAWRVPTPPQMHNRGNYII
                     SLGSLCRWLGQQAEALGVEIYPGFAAAEVLYGDDGRVAGVATGD"
BASE COUNT          955 a         2055 c         2129 g          956 t
ORIGIN      
        1 ccctggcaga agttctggca cgtgaccctg ccgatgctct cgcccatcat cgccgtggtg
       61 atgaccttct cggtgctgtt cacgttcacc gacttccagc tgatctgggt gctcacccgc
      121 ggcgggccga tcaacgcgac gcacctgatg gctacgctgt cgttccagcg cgccatcccg
      181 ggcggcgcgc tcggcgaggg cgcggccatc gccaccgcca tgatcccgtt cctgctcgcg
      241 gcgatcctgt tcagctattt cgggctgcag agacggcgct ggcagcaggg gatgaaggat
      301 tgagcggaag ggcgtgaccg atgaaggacc aggagctcgg cagcggaatg cgctacctcg
      361 tctcagtccc gaggcgggtg gtcacggtgt acgtcccgct gctggtgttc ctgttcgtgc
      421 tgctgttccc gttctactgg atggcggtca ccgcgatcaa gcccaacgcc gagctgctgt
      481 ccaccggcgg caatccgttc tgggtgatcc ggcccacgct ggagcacttc gagaagctgc
      541 tgctgcgcac cgcatacccg agctggatgc tgaacaccat gctgatcgcg gtgatctcga
      601 ccttcgcgtc ggtcgcagcg agcgtgctcg ccgcctacgc catcgaacgc ctgcgcttcc
      661 gcggctcgcg ccaggtcggc atggcgatct tcctcgcgta tctggtcccg cccagcatcc
      721 tgttcatccc gctggcgacc atcgtgttcc agctcggtat cttcgacacc aagtgggcgc
      781 tgatcctcac ctatccgacg ttcctgatcc cgttcagcac ctggctgctg atgggctact
      841 tccgctcgat tcccttcgag ctggaggagt gcgcgctgat cgacggagcc agccgctggc
      901 agatcctggt caagatcgtc ctgcccctgg cggtgccggg gctgatctcg gccgggatct
      961 tcgctttcac cctgtcgtgg aacgagttcc tgtacgccct caccttcatc tcctcgtcgg
     1021 agcacaagac ggtgccggtc ggggtgatca ccgagctggt ggagggcgac gtgtaccatt
     1081 ggggcgcgct gatggccggc gcgctgctgg gctcgctgcc ggtggcgatc ctgtactcgt
     1141 tcttcgtcga gtactacgtg gcgggcatga ccggtgccgt gaaagaatag ccggtgccgt
     1201 gaaagaatag ccggcgccgt gaaagaatag ctcctcggcg ccgggcggcc ttccgctgca
     1261 gtggacaggc atccggcgcc ggcgtagctt cccccaaaac acagcgaaag gcgcgcaccg
     1321 catgtcgcaa cccatccgga tcctccacat cggcccggcg aagtccgagg ccttcgacac
     1381 ctcctcgctc gagcagcaga gccgcgccgt ccgcctcgcc ggcggaaccg tgatccgcta
     1441 cgagggccgc agcaccgcgg agctggtgca ggcgctgcgc gaggtggacg cggcgatggt
     1501 tcagggccac gttttcgatc aggccgcctt cgcgcacatg ggcctgcacg gccgctgcaa
     1561 gggcctggcc tcgttcggcc acggctacga ccatctggat ctggacgcgg cgaccgccaa
     1621 cggcgtcgtc ctggccaaca ccgcctcgtt cggcaccgag gaggtgtcga accacaccat
     1681 catgcacttc ctggtctgcg ccaggaagtt cgtgctgcac gacaagctgg tgcgcagcgg
     1741 cgcctggacg cgcaagaacc tggcgccgat gggtcacgtc gccgggcagg tgttcggcat
     1801 cgtcggcctg ggcgacatcg ggcgcgccgt ggcccgcaag gccaaggcct tcggcctgca
     1861 ggtgatcgcc tacgacccgt acgtggcgtc ctgggatgcg cttgagtacg gcgtggagat
     1921 gatcgactcg ctggaagcgc tgtgccggcg ctccgattac atcagcgtcc acgtctatct
     1981 caacgccgag acgcgccatc tcatcggccg cgcgcagttc gccgtgatga agcccagcgc
     2041 gtacctgatc aactgcgcgc gcggcggcgt cgtggacgag gccgcgctga tcgaggccct
     2101 gcagcaacgg cgcatcgcgg gcgccggcct ggacgtgttc gagaaggagc cggtcgcctc
     2161 cgacaacccg ctgctggcga tggacaacgt ttcgctcacc aatcactacg ccagctattc
     2221 ggagaccgcc tgggagcgcg cgcagacgca gctcggcgag gaggcggtgc gcatcgccac
     2281 cggtatgtgg ccgatgtcgc tgatcaaccc ggacgtgcgg cacgtgatcc cgccgcgggc
     2341 gccggcgcgc tcgtgggaag tgctcgcgcg cgagctggcg gccgccgggc accggtgacg
     2401 acgaccgggg cgcgggcgtg accggagacg gccgatgaga atcaacaagg cgctggagct
     2461 gctggagcag cgccagccgg tgtacttcga gttcgtcgat gccgaggacg cgggcgagta
     2521 cgacggcggc cgtgcgctgg cgcagacttg ggccgactac atcgcctacg acatggagca
     2581 cagcgcgctc gacgtgcgcg ggctgatgcg gttcatgcgc ggcctggtcg acggcggccc
     2641 gacccggagc ggacaccgga cccccgcggt gatcgtgact ctcccgatcg acggcacgga
     2701 cgagcagtcg gtgcgcgcca acgggtggat gatcaagcag gtgctcgcca ccggcgtgca
     2761 cggcctgctg ctgtgccacg ccgagagccc ggaggcggtg cgcgccttcg tcgaggcggc
     2821 gcgctacgcc tttcatagca gcggcggcca cgctggcgcc ggccatgctg gcgccggccc
     2881 ggaactcggg cccgggcgcc gcgggcacgg cggtcagaca cgcgcggcgg agatctgggg
     2941 cgtggcgccc ggcgagtacc tggcccgagc cgatccctgg ccgctcgacg cgcgcggcga
     3001 gctgctgctc gggctcaagg tggagaacaa gcgtgcgctc gcagccgtgg aacgcaccac
     3061 gcgcgtgccg gggatcgcgt tcgccgagtg gggccccggc gacatgggca tgtcgatggg
     3121 gtatccggac cagcacgacg aaccctatcc ggccgagatg cgcgccgcgc gcgctcgcgt
     3181 catgagcgcg tgcaaggtcg ccggcctcgc cttcctggag gcggtcacgc cggagaacgt
     3241 gatcggtcgc ctcgaggagg gggtgacgat cggctgcggg ccgcgtgcgc aggaagcggc
     3301 ggacatcggt cgcaggtaca cgcggcgcgc gctgccctgg tgagcgacgt gatgccgcac
     3361 accacaccgc cccggcgcct gcggcgccac cccgcctcgt gagaggcggg gattgaggta
     3421 gttcaaatcg cgccttcagg gcgcatccgg ccgacaagga ggcagggatg aaccgattca
     3481 cccgacgccg cttcctcacc gccggtaccg tggcggccgc cgcggcggcc gtgccgggac
     3541 tcgcgccggc ccaggtcgcg ccgctcaggt tcgagcccga gaaaggcgca acgctgcgcg
     3601 tgctgcgctg gaagcgcttc gtgcagggcg acgaagatca gttcatggcc aacacgcggc
     3661 gcttcaccca agccaccggc gtccaggtgc gcgtggacag cgagaacttc gaagacctgc
     3721 gccccaaggc cgcggtcgcg gccaacgtgg gcgccggacc cgacatcatc atcagcaccg
     3781 acgagcaacc gcagctctac ccggacaagc tgctcgacgt gaacgacctg gcgcagtacc
     3841 tggggcagaa gtatggcggc tggttcgagg tgtgcccgga gtactgcacc taccagggaa
     3901 gatggatcgc catcccgacc ggcgttgcag gcggggccat ggtgtatcgc cgcagcatgc
     3961 tgcgcgccgc cgggttcgag cagttcccga aggatctgga cggttttctg aagctgtgcc
     4021 gggcgctgaa ggccaaggcg gcgccgcccg ggttcgcgct ggcgcacgcc accggcgacg
     4081 ccaacggctg gactcactgg ctgctgtggg cgttcggcgc gcggcaggcc gacgaacaga
     4141 gccggctggc gctcaactcc aaggagacgg tggcggcgct ggaatacgcg aaggagctgt
     4201 atcagacctt cgcgccggga acgctgtcct ggctggatcc gagcaacaac aaggccttcc
     4261 tcgccggcga gatcggcctc acgatgaacg gcatctcgat ctactacacc gccaagacca
     4321 gcgccgaccc gaaggtgaag gcgatggcgg cggacatcga gcacgcggct ttcccgatcg
     4381 ggcccatcgg gcgcgacgtg cacggggcgc tcatgttccc cggggcgatc tacaagtact
     4441 gcaagtatcc gaacgcggcg caggagtacc tgcgcttcat catggagcgc gagcaatacg
     4501 agccgtggca gcgggcgtcc atcggctaca tctcacacac gctgcgcgcc tacgacgcca
     4561 atccggtgtg gagcgaagat ccgcaacacg tgttcttccg cgatatcgcc aagggcgcgc
     4621 ggcataccgg atacgcggga aggctgggga aggaatcggc ggcggtgctg gccgatttcg
     4681 tcatcgtcga catgttcggg gaagcgtgca ccgggcgata cgcccccaaa gaagcgctgc
     4741 aacgcgcgga gaagcgggcg cggcgccact accgcagatc atgaggtgag cgcggccgcg
     4801 acgcggtcgc ggatctcctc cacgctgccg ctgccggaca ccttgcagta cttgggcgcg
     4861 cgcggatcgc cgctggcggc ccaggccgag tagtagtcca ccaggtgcct ggtctcggcg
     4921 tgatagatct cgagccgctt gcgcaccgcc tcctccttgt cgtcctcgcg ctcgatcaag
     4981 tcctcaccgg tgaggtcgtc cttgtccggc accttcggcg ggcgcaggcg cacgtggtag
     5041 gagcgccccg agcctgggtg cacgcgccgt ccggacaggc gctcgatgat ctcggcgtcc
     5101 tccacgtcca gctccaccac gtaatcgacc ttcacgccgg cctgctccat ggcctcggtc
     5161 tgcgtaatgg tgcgcggaaa gccgtcgaac aggaagccgt tggcgcagtc gggctgggcg
     5221 atgcgctgct tcaccaggct catgatcacc acgtcgggca ccagctgccc ggcgtccatg
     5281 tacttcttga ttctcaatcc tgcggggctg ccgacgcgca ccgccgcgtg cagcatgtcg
     5341 ccggtggaga tctgcgggat gccgtagtgc tggcagatgg cgacggcctg cgtgcccttc
     5401 ccggcgcccg gcgcgccgag caggatcagt ttcatgcgga tgtcctccgg tgaatgctgc
     5461 cgcgaagagg cgcaattgga agaggcgcaa ttatacgacc tgcgctgcgc catgtgccgg
     5521 cgtcggctga ccgatccggc acggaagcgg cggtgcggcg tggatgcaac gcgcagccac
     5581 accgaaagcg tttatcatcg cggcatctgc ggcgagcgca tgccgtgcgg agaagcgatt
     5641 ggccgagcgc gagttcatgg agtacgacgt ggtgatcgtg ggcgcggggc cctccggcct
     5701 cgcggcggcg attcgcctca agcagctcgc cgccgagctg tcggtgtgcg tgctggagaa
     5761 aggctccgag gttggcgcgc acatcctgtc tggcgcggtg ctcgagccgc gcgcgctcga
     5821 cgagctgctg ccggactgga aggcgcgcgg cgcgccgctg gacacgcccg cgcgcgagga
     5881 tcgcttcctg tacctcagcg ccgcgcgcgc ctggcgcgtg cccacgccgc cgcagatgca
     5941 caaccgcggc aactacatca tcagcctcgg cagcctgtgc cgctggctcg ggcagcaggc
     6001 cgaggcgctg ggggtggaga tctacccggg gttcgcggcc gccgaggtgc tgtacggcga
     6061 cgacggacgc gtggccggcg tggccaccgg agacc
//
LOCUS       DASPGE010000149        21878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_862990, whole genome shotgun sequence.
ACCESSION   DASPGE010000149 DASPGE010000000
VERSION     DASPGE010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21878
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862990"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VD791_04680"
     CDS             <1..886
                     /locus_tag="VD791_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HXV07299.1"
                     /translation="VQQKGSLVDAWKTRFDFSHDKPLTAEEIREIERLVNDEIRHNHA
                     VEARLMKYDDAIKHGAMALFGEKYGDEVRVIGMGEFSTELCGGTHVKRTGDIVFFKIV
                     AQSAVAAGIRRVEAVTAQGALDYVQQQTTALEGVAEMLKAHPMEIGSRLSQIMDNVRA
                     LEKELARLKSKLAASQGDDLADQAVAVGAARVLAAKLDGADVKSLRETVDKLKDRLKS
                     AAVVLASSEGGKVTLIAGVTDDLTAKVKAGELVNFVAQQVGGKGGGRPDLAQAGGTDP
                     AKLPQALASVKRWVEERL"
     gene            complement(949..2199)
                     /locus_tag="VD791_04685"
     CDS             complement(949..2199)
                     /locus_tag="VD791_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXV07300.1"
                     /translation="MAEQPAQELFLGRQPILDRGQNLAAFELLFRAGHFNGAQIEDDV
                     FASATVINHAFSELGMEAVLGKYPGFINLSAPLIMSDVIELLPKNKVVLEILETVRVT
                     DALAQRCRALKQIGFTLALDDYTGREEVFKPLLDVVDIVKVDVQHMDEQALERTTASL
                     KKLPVRLLAEKLDTRAQVERCLELGYELFQGYYFARPSIITGKRLTRSEAALMRLLGL
                     LLSGADTQKIEQVFEHDPDLASNLMKLLNSVAIAGQIRIQSLRQALAVLGRERLLRGL
                     HVLLFTLSSAPGAEFPSPLLLLAATRGRMMELIARALRPQDSAFHDRAFVAGILSLVN
                     ALLGMPMSEILGSMPVHAEVRAALVERSGHLGAMLSLVEALEEAELIGIERALDRVPG
                     LEHGQVMGLQIEAMRWANSIGEPA"
     gene            complement(2219..3595)
                     /locus_tag="VD791_04690"
     CDS             complement(2219..3595)
                     /locus_tag="VD791_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HXV07301.1"
                     /translation="MSRVPPEIFKAYDVRGVVGKTLTPEIAELIGRAVGSRARALDQT
                     TVCIGRDGRLSGPSLCAGLARGLQAAGVDVIDVGRVATPMLYFATFHLQTGSGVMVTG
                     SHNPPDYNGLKMMVAGETLSGDAIQGLRERIERDDFASGSGGYRQTDVTQAYFDRIAG
                     DVKLARPLAIVVDCGNGVPGAFAPELYRRLGCKVRELFCEVDGTFPNHHPDPSQPENL
                     EDLMHALASGEEELGLAFDGDGDRLGVVTKAGKIIYPDRQLMLFAADVLSRNPGAQVI
                     FDVKSTRNLFSWIDAHGGRPLLWKTGHSLIKAKMKETGALLAGEMSGHVFFKERWYGF
                     DDGMYAGARLLEYLSRCPDIDAEFARLPDSVNTPELQIRLEEGENYALIGKLQRSARF
                     EGAREVITIDGLRVEYADGFGLARASNTTPVVVLRFEADNPAALRRIQEDFRRVLSAG
                     APQATLPF"
     gene            complement(3624..4604)
                     /locus_tag="VD791_04695"
     CDS             complement(3624..4604)
                     /locus_tag="VD791_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HXV07302.1"
                     /translation="MAAPAIYRAPRWLPGAHLQTIYPYLFRRLPLPPLRRERWETPDA
                     DFVEVDWLEGRAEAPLMVLFHGLEGCSRSHYARALLRAAQVRGWRAVVPHFRGCGGAP
                     NRLARAYHSGDYAEIDWMLRTAARISPAAPRFALGVSLGGNALLKWLARERQAAAQVV
                     TRAAAVSAPMDLTIAGRLLGRGFNRVYTWNFLKSLKRKSLAKLRRYPGLYAAHAVARA
                     RDLYQFDNLVTAPLHGFKDADDYWTRASSKPDLVHIALPTLIVHAGNDPFLPGMHLPA
                     AQALPSCVTLEVPAEGGHAGFVCGRFPGNMDWLPARVLGFFEEASAPAMG"
     gene            complement(4621..5037)
                     /locus_tag="VD791_04700"
     CDS             complement(4621..5037)
                     /locus_tag="VD791_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXV07303.1"
                     /translation="MTLSSSEQAEAAFYNAFEKADLGAMMAVWAEDEEILCIHPGGQR
                     LMGMDAVRESWRALFAPGPHMRFRLLDVRVQSGRMLAVHNLYERISVVGQRKAHLALA
                     TNVYVLTPSGWRMLVHHASPLPPDAAAPDAPVGTLH"
     gene            complement(5034..5249)
                     /locus_tag="VD791_04705"
     CDS             complement(5034..5249)
                     /locus_tag="VD791_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HXV07304.1"
                     /translation="MEQGKSTDGARRVEVDRADLPLHCPTPQSMLWNAHPRVFLPLEQ
                     RGEALCPYCGTLYVLKGHVLEDGARAA"
     gene            complement(5264..6184)
                     /locus_tag="VD791_04710"
     CDS             complement(5264..6184)
                     /locus_tag="VD791_04710"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HXV07305.1"
                     /translation="MSMADRDGLIWYDGKLVPWRDATTHVLTHSLHYGLACFEGVRAY
                     KTAGGTAIFRLKEHTDRWFNSCHIFGMKMPFDRETLNAAQREVVRANKLESCYIRPIS
                     FYGSEKMGVSPKGARVHVAIAAWPWGAYLGEEGLEKGIRVKTSSYARHHINVTMCRAK
                     FSGTYANSILANQEAVEHGYDEALLLDVDGYVAEGPGENLFIVKHGKLYEPELTSALI
                     GITRDAVTQLARDAGLEVQAKRITRDDVYIADEAFFTGTAAEVTPIRELDGRVIGAGK
                     RGPITEKLQSMFFDVVNGRNQKYVHWLTPV"
     gene            6305..6958
                     /locus_tag="VD791_04715"
     CDS             6305..6958
                     /locus_tag="VD791_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HXV07306.1"
                     /translation="MTVQIEVISDVVCPWCFIGKRRLEQALARLREAPTAPTVSVAWL
                     PFQLNPDLPETGMDRADYLRRKFGHAAPQIYARVAGAGRSAGIAFAFDAIARQPNTVA
                     AHQLIALAAPAGRQDQMVETLFRAYFLEGADLTQRATLVALAQRSGIDPARATACLDD
                     PLQRQAVLDAERDGRELGVEGVPLFLFNRRLAVSGAHEPQVLLAAIDQAARDAAEVT"
     gene            7122..8492
                     /locus_tag="VD791_04720"
     CDS             7122..8492
                     /locus_tag="VD791_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXV07307.1"
                     /translation="MPQVTAAAASVSVSAAPVAEETRVLQDKTSAVPASAAARRGSWL
                     ERLGRAAVGEDDDDELRLRKTLLIFASGLMNMAAIVWLAIYWALGLKLPTTIPLAYQL
                     LSALILAVYLKTRNFDFFRVAQLSLFLFAPFVIQWSIGSFVSSSGIVLLALLAPVGAM
                     VVYGPRESIPWFVAYVALTALSGAFDYYLADGNLSGVPMRTVAVFFVLNFTILSSIVY
                     LLLRYFVQQKDTFQAELARQHELVQLEQRKAEQLLTTILPPHIAQRLKQDQTTIADGF
                     ADVSVMFADIVNFTTLAEELTPKEVVSFLNDVFTRFDELAEKHGVDKIKTIGDAYMVA
                     GGVSGDDTQYVSSVADLALEMRDLTRNDEVIRRYNVSFHVGIATGPVIAGVIGVKRFI
                     YDLWGDTVNIASRVTAEAPSGEIYVDKTTYRRLGTRYTFSEPQELMFKGKGKMTVYQL
                     LGRRAS"
     gene            complement(8557..11286)
                     /gene="glnE"
                     /locus_tag="VD791_04725"
     CDS             complement(8557..11286)
                     /gene="glnE"
                     /locus_tag="VD791_04725"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HXV07308.1"
                     /translation="MQSPSAPPRPEQGAPEQLLDPEQLLDRAGQCSRFLRRLREHPPR
                     SFDPARLHQPLTGAVIGALLHAQPAADEAALLRALREVRRTVMLHLITRDLTGLADLA
                     EVMDTATALAEHSVRFALSHLDDALDAAHGRPVGADSGAVQHLHVVAMGKLGGAELNV
                     SSDIDLIFLYPEEGQTSGGRPLSAHEYFTRLGRKLINALAQYTADGHVFRVDMRLRPY
                     GDSGPLAMSFAMLENYLHTQGREWERYAWVKAREITGRPAPELTQLVTPFVYRRHLDF
                     SAFASLRELHAQIRTEVERRELHDNIKLGPGGIREIEFVAQVFQIVRGGRDADLRLRP
                     TLAALARLGERRLLPVQAVDELRAAYVFLRNLEHRLQYLEDQQTQMLPASAEDRALIA
                     RAMGYADAAAFGEALERHRANVTRQFEAVFAGAVGEVAEHPLSALWHGTLDQDQALAR
                     LTALRYENAAVTVARLREFHDGPLYRRMSASTQARVDRLVPRLLEAAAASERPGQTLE
                     RLARVVESIGRRESYLALLLEYPAALQRLAGLAAASPWAADYLARHPMLLDELIAPDA
                     ISAPDWLTLGARLAAELDAADGIERQMDLLRHFKQVQTMRLLTQDLAGALPLETLSDH
                     LSDLACLILQQVLRLAWSHLRTRHRPDARFAVVGYGKLGGKELGYASDLDLIFLYHDP
                     HPDAPENYARLAQRMNTWLTSFTAAGVLYDTDLRLRPDGASGLLVSRLDAFAQYQRSK
                     AWVFEHQALTRARFVAGDADIGRRFEQLRVEILRLPRELPRLREEVAAMRARMLEGHP
                     NTSGLFDIKHDRGGIVDVEFVVQYLVLGHAHTHAELTGNIGNLALLRLAGRLGLIDSH
                     AAEAVHHSYREFRRMQHALRLQGERYARVERDKVAGLVRPVLALWRAVFEGAV"
     gene            11543..15343
                     /locus_tag="VD791_04730"
     CDS             11543..15343
                     /locus_tag="VD791_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HXV07309.1"
                     /translation="MTRRPAVQLMRHGSLWLYRAVMLTLVGGTVLAGGAVLLLRYWVL
                     PGVDAYRPQIEAALSRAANERITVGGVEGQWDGIRPRLALREVRVFDRAGVERLALAK
                     VDGTLSWTTLLAGEPRFHSIELTGLSVEVRRDRAGGWLIAGMPLTMQGGDSGFGDWLQ
                     EQHRIAVRDSALTWVDETLGGIALPLTDVQLEVERRFGAHRFDLRATPPLDVAAPLGL
                     RGELRHPGRRASGTLYLQVGYADLVTLRQWLPLPVYAARGAGALEVSMDLEEGTLRSV
                     SAQVALSEVSVRLDPDLPPLELSRMSGRLGWAGNGRKLEVLASNLAFTTPDGLHLPPA
                     DLRYRRWGAAGDPATRSELRFDALDLAAVTRLVDRLPIDAGVQARLTEMNPRGTLRGF
                     EVGWTGPWGGHGDYQASGAFERVSVAASGYLPGFSSVSGQLQADQRGGSMSLRADQSE
                     LAMPHVFVEPLPLDSLAGTLSWSMQSDGPLLRIESLVFANQHLAGKVAGTYRAIAGQP
                     GAVDLTGSLERGDGRQAWRYVPLRVHARVRDWLRESVVSGHTRDVQFRLRGDLARFPF
                     ADGKSGLFEVVTRFEGGTLAYAPGWPAIEGLRGEMMFRNAGMNVVAEAAQVYGTRMAS
                     GRATIADLSVDDPVLEVRMETEGPTEDYLRFVEHSPVDRMIGGFSRGMKAFGRGALVL
                     GLQLSLKHAEQVRVSGRYRFAANTLDPGHGVPPLEQLSGQLSFSERDVSLRGGSARIL
                     GMPARFIADRGGKGALVIRGEGRAEAAGLRQVLDHPGVAALGGATGWHSTLAIQDGGY
                     DLVIASDLRGLSSSLPAPLAKDAAADLSFRLQRRSINDRHELVVLTVGDIVSAQLLRG
                     RDAAAGVLRGEVRLNAPAPAPERDGLWLAGKLNGLDLDRWRTLVGQGASALPISLSGI
                     DLRVAELRAFGREWHEVALKVSTTPARWEGSLVSWEAAGTLFWTPAGPGALTGRFTRL
                     HLPPAPSELRAAQAGGGAGYPALDVTAEDFRLGESQLGRLTLVATPENGEWRIRQLDL
                     RSPEGTVSMKGAWQALPAPLTRLDIKGEVNDIGGYLARLQLPKGIDGGSARVQGQLSW
                     SGSPQAVDWPTLSGALQLEAKKGQFLKVEPGIGKLIGVISLQSLPRRVALDFRDVFSD
                     GFAFDEISASAAIARGVARTQDFSMAGPSARVEMKGEINLAGETQRLDVKVVPSVSES
                     VALGAAIVNPAVGIATLLAQKALKDPIGAMVAFEYEVSGTWDDPMVMKKKREPARPQP
                     QGRK"
     gene            15486..16943
                     /gene="tldD"
                     /locus_tag="VD791_04735"
     CDS             15486..16943
                     /gene="tldD"
                     /locus_tag="VD791_04735"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HXV07310.1"
                     /translation="MTAVTPSEVLFATAHQTLLAPYGLDAGRLTRVFGEIMTHQIDYA
                     DLYFQYSRGESWSLEEGIVKSGSFSIEQGVGVRAVSGDKTAFAYSDDISLPALQSAAA
                     ATRAIARQGGEARTAVARRSHGRELYLPHDPIASLDAPRKVALLERLEGFARARDPRV
                     KQVMASLTGEYEVVLVARSDGVLAADVRPLVRLSLQVIAEEGDRREQGSAGGGGRFDY
                     VYFDDEMLRGYAEKAVDQALINLRARPAPAGTMTVVLGPGWPGVLLHEAVGHGLEGDF
                     NRKGTSAFSGRIGERVAAPGVTVVDDGTLPRRRGSLNVDDEGNATQSTVLIEDGVLTG
                     YLQDALNARLMGMAPTGNGRRESFAHVPMPRMTNTYMLGGARDPQEIIGSVKRGLYAV
                     NFGGGQVDIVSGKFVFSASEAYLIEDGKVGDPVKGATLIGNGPDALTRVGMIGNDMSL
                     DTGVGTCGKEGQSVPVGVGQPTLRIDGLTVGGTQS"
     gene            16940..18055
                     /locus_tag="VD791_04740"
     CDS             16940..18055
                     /locus_tag="VD791_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07311.1"
                     /translation="MILILNENADPAGPEYAALMEALRQLPGIQVKAHRIEGELRAVT
                     EIYLIGDTKQLSPEDMRALPLVERVVRVSEEYRVLGRHKDDVRPTWFEYQGLRFGQDT
                     LHLFAGLCAVDTREHVERMCRALRDNGQVCTRMGAYKPRTSPYAFQGHGRACLNYVFE
                     IAGKYGIRVVAMEVTHEAQVDEIRDALRETESPTGAMLQIGTRNTQNFELLKAVGRQR
                     QFPVLIKRGFGITLDESLNAAEYLASEGNQRVIFGLRGMKTHMGDPHRNFVDFVHVPV
                     VKRLTRMPVCIDPSHSVGSRARGVDGVLDLMHATAQGVIAGANMVLVDFHPEPSKALV
                     DGPQALLLSELPQFVEDVAIAREAYLKRTELARRKAL"
     gene            18193..18717
                     /locus_tag="VD791_04745"
     CDS             18193..18717
                     /locus_tag="VD791_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXV07312.1"
                     /translation="MIEPAAQYSLLKDLEQLGEGTALAEQIYSMIGHSRFFDDFSRED
                     VQELAGFMQVYRAEPGAMIIREGDVDDYMLLIIQGKVDIVKTDKRGVIQAMTSVGPGM
                     TLGEMSMIDGEPRFATCIAIEPTTFAVLSRDDMVRIILEEPSLGAKLLIKLVTLLSQR
                     LRQTSSTLLHYMER"
     gene            18714..19589
                     /locus_tag="VD791_04750"
     CDS             18714..19589
                     /locus_tag="VD791_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010114346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HXV07313.1"
                     /translation="MTDPVASRRRFAALLALAAAAPCALARSAPVADDRGKVLRLQFP
                     ARRIAALSPHLAELTFDAGAGGALIAAVRGSDYPPEVKRLPTIGDAAGLDLERILALR
                     PDLVLGWLSGNRATDIERLERLGLPVFVSEPRRLSDVPATLRRMGTLAGTRAAAEHAA
                     RAFEARLARLTPPSVSPPVSVFVEIWHTPMMTVNGAHLISEVLAACGGHNVFADLPAL
                     AASVGVEAVLAADPQVIIATTPPGQDALAAWQALPRLRAVRAGRLHAIDPDLLTRATP
                     RILDGVEQVCAWLRP"
     gene            complement(19602..20303)
                     /locus_tag="VD791_04755"
     CDS             complement(19602..20303)
                     /locus_tag="VD791_04755"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HXV07314.1"
                     /translation="MKYRNELVREALAAEYALGTLQGRARRRFERSLKDDPALRRYVT
                     SWQERLAPLDATVAPVQPPARVWRAIQRRVTAAPRGWAALWDSLGFWRGAAFAALAAA
                     LISTASLGVLQPERAELMVVVMSDERAEPRMTVSWETDGRGDKRMRIRVLGHAEMAPD
                     TAWELWMLPGGDRKPLSLGLITTHETQSVLVPRELATAINAAWGLAMSVEPKGGSPTG
                     LPTGPVLYKGQCTKL"
     gene            complement(20311..20865)
                     /locus_tag="VD791_04760"
     CDS             complement(20311..20865)
                     /locus_tag="VD791_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV07315.1"
                     /translation="MPDYDPIGELMGRCALRDAQAFAQLYRHTAAKLYAVALRILRRE
                     DWAEEVLQESFVNIWNHVGEYSAQKSAPMTWMTAIVRNRALDWLRRPRQEHGSEDYDL
                     LVEALADDRPGPEQALQSERAARALAECLKRLSSNQRQSIALAYAHGLSHGELARHLR
                     QPLGTVKTWIRRGLEKLKSCLDDH"
     gene            complement(20917..21480)
                     /locus_tag="VD791_04765"
     CDS             complement(20917..21480)
                     /locus_tag="VD791_04765"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HXV07316.1"
                     /translation="MGHRLTRIYTRTGDGGSTGLGDGTRVQKDSVRVEALGAVDELNS
                     AIGVLLAEPLPEDVRGQLTAIQHDLFDLGGELSIPGYTRIAGAHVQQLEALLDAHNAG
                     LEPLKEFILPGGSRSAALCHLARAVCRRAERRVVSLASAETLSAYCQQYLNRLSDLLF
                     VLSRVLNRSAGQPDVPWRKDTGPGKPG"
     gene            21688..>21878
                     /locus_tag="VD791_04770"
     CDS             21688..>21878
                     /locus_tag="VD791_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXV07317.1"
                     /translation="MTRSRALAAAAAAAALSLGQSLSAAQGDPLAAVHKKQMCEGCHG
                     KEGYRTAYPTVYSAPKLGG"
BASE COUNT         3254 a         7551 c         7713 g         3360 t
ORIGIN      
        1 cgtgcagcag aagggctcgc tggtcgacgc ctggaagacg cgcttcgact tctcgcacga
       61 caagccgctc accgccgagg agatccgcga gatcgagcgc ctggtgaacg acgagatccg
      121 gcacaaccac gcggtcgaag cgcgcctgat gaagtacgac gacgcgatca agcacggcgc
      181 catggccctg ttcggcgaga agtacggcga cgaggtgcgc gtgatcggca tgggcgagtt
      241 ctccaccgag ctgtgcggcg gcacgcacgt gaagcgcacc ggcgacatcg tcttcttcaa
      301 gatcgtggcg caatcggcgg tggcggccgg catccgccgg gtcgaggcgg tgaccgcgca
      361 aggggcgctg gactacgtgc agcagcagac caccgcgctg gaaggcgtgg ccgagatgct
      421 gaaggcgcac cccatggaga tcggcagccg cttgagccag atcatggaca atgttcgcgc
      481 gctggagaag gagctggcgc ggctcaaatc caagctcgcc gcctcacagg gcgacgatct
      541 ggccgatcag gcggtggcgg tcggggccgc gcgtgtgctg gcggcgaagc tcgacggcgc
      601 cgatgtcaag agcctgcgcg agacggtgga caagctcaag gaccggttga aatcggcggc
      661 ggtggtgctg gcctccagcg aaggcggcaa ggtgacgctg atcgccgggg tcaccgacga
      721 cctcaccgcc aaggtcaagg ccggcgagct ggtgaacttc gtggcgcagc aggtcggcgg
      781 caagggcggc gggcggcccg atctggcgca ggccggcggc accgatccgg ccaagttgcc
      841 gcaggcgctg gcgtcggtga agcgctgggt ggaagagcgg ttgtagccgc cttcgcgccg
      901 cgctgccgaa acccgctgca tggccgcgct gcgcggctcg tgactgctct acgccggctc
      961 gccgatcgag ttcgcccaac gcatcgcctc gatctgcagg cccatgacct ggccgtgctc
     1021 gaggccgggc acccgatcca gggccctctc gatgccgatc agctcggctt cctcgagcgc
     1081 ctccaccagc gacagcattg cgccgaggtg gcccgagcgc tcgaccagcg ccgcgcgcac
     1141 ctcggcgtgc accggcatgg agcccaggat ctcgctcatc ggcatgccga gcagcgcatt
     1201 gaccagcgac aggatgccgg cgacgaaggc gcgatcgtgg aatgcgctgt cctgcggtcg
     1261 cagggcgcgc gcgatcaact ccatcatgcg cccgcgcgtg gccgccagca gcagcagcgg
     1321 gctggggaac tcggcgccgg gcgcgctgct cagggtgaac agcagcacgt gcagcccgcg
     1381 cagcagccgc tcgcgcccga gcacggcgag cgcctggcgc aacgactgga tacggatctg
     1441 gccggcgatc gccaccgagt tgagcagctt catcaagttc gaggccagat ccgggtcgtg
     1501 ctcgaatacc tgctcgatct tttgcgtgtc ggcgccggac agcagcaggc cgagcaggcg
     1561 catcagcgcg gcctcgctgc gggtcaggcg cttgccggtg atgatcgagg gacgggcgaa
     1621 gtagtagccc tggaacagct catagcccag ctcgaggcag cgctccacct gcgcccgggt
     1681 gtccagcttc tcggccagca ggcgcaccgg gagcttcttc aggctggcgg tggtgcgctc
     1741 gagcgcctgc tcgtccatgt gctgcacgtc gaccttgacg atgtccacca cgtcgagcag
     1801 gggcttgaac acctcctcgc gcccggtgta gtcgtccagc gccagggtga aaccgatctg
     1861 cttcagcgcc ctgcagcgct gggcgagcgc gtcggtgacg cgcaccgtct ccaggatctc
     1921 cagcaccacc ttgttcttgg gcagcagctc gatcacgtcg ctcatgatca gcggcgcgct
     1981 caagttgatg aagccgggat acttgccgag gacggcttcc atccccaact cgctgaacgc
     2041 gtgattgatg acggtggcgc tggcgaacac gtcgtcctcg atctgcgcgc cgttgaagtg
     2101 gccggcgcgg aacagcagct cgaaggccgc caggttctgg ccgcgatcga ggatcggctg
     2161 gcgcccgaga aatagctcct gggccggctg ctcggccatg atcgcgcgcg caaccccgct
     2221 agaacggcag ggtcgcctgc ggcgcgcctg cgctcaggac cctgcggaag tcttcctgga
     2281 tgcggcgcag cgcggccgga ttgtcggcct cgaagcgcag cacgaccacg ggcgtggtgt
     2341 tggaggcgcg cgccagcccg aatccatccg cgtattccac gcgcagcccg tcgatggtga
     2401 tcacttcgcg cgccccctcg aagcgtgcgc tgcgctgcag ctttccgatc agggcatagt
     2461 tctcgccttc ctccaggcgg atctgcagct cgggcgtgtt caccgagtcg ggcaggcgcg
     2521 cgaactccgc atcgatgtcg gggcagcggc tcaggtactc gagcagccgc gcgccggcgt
     2581 acatgccgtc gtcgaaaccg taccagcgct ccttgaagaa cacgtgcccg ctcatctccc
     2641 cggcgagcag cgcaccggtc tccttcatct tggccttgat cagcgagtgg ccggtcttcc
     2701 acagcagggg ccggccgccg tgcgcgtcga tccaggagaa caggttgcgc gtggacttga
     2761 cgtcgaagat cacctgcgcg ccggggttgc gcgacagcac gtcggcggcg aacagcatga
     2821 gctgacgatc gggatagatg atcttgcccg ccttggtgac cacgccgagc cggtcgccgt
     2881 cgccgtcgaa cgcgagaccg agctcctcct cgccgctggc gagcgcatgc atcaggtcct
     2941 cgaggttctc cggctgcgac ggatccggat ggtggttggg aaaggtgccg tccacctcgc
     3001 agaacagctc gcgcaccttg cagccgaggc gccggtagag ctccggtgcg aaggcacccg
     3061 gcacgccgtt gccgcagtcg acgacgatcg cgagcgggcg cgccagcttg acgtcgccgg
     3121 cgatgcggtc gaaataggct tgcgtcacgt cggtctggcg gtagccgccg ctgccggagg
     3181 cgaagtcgtc gcgctcgata cgctcgcgca ggccctgaat cgcgtcgccc gacagcgttt
     3241 cgcccgcgac catcatcttc aagccgttgt agtccggcgg gttgtggctg cccgtcacca
     3301 tcaccccgga gccggtctgc aggtgaaagg tggcgaagta gagcatcggc gtggccaccc
     3361 ggccgacgtc gatcacgtcc acgcccgcgg cctgcagccc gcgcgccagg cccgcgcaca
     3421 gggacgggcc ggacaggcgc ccgtcgcgcc cgatgcacac cgtggtctgg tcgagcgccc
     3481 gcgcccgcga gcccaccgca cgcccgatca gctcggcgat ctccggcgtc agggtcttgc
     3541 ccacgacgcc gcgaacgtca taggccttga agatctcggg cggtacgcgg gacatggggg
     3601 tgtcgcgaca agtgtcgcgc gcattatccc atagccgggg ccgaggcttc ctcgaagaag
     3661 cccagcacgc gcgcgggcaa ccagtccatg ttgccgggaa accggccgca gacgaagccg
     3721 gcgtgcccgc cctcggccgg gacctcgagc gtgacgcagg agggcagcgc ctgcgcagcc
     3781 ggcagatgca tgcctggcag gaacggatcg ttgcccgcat gcacgatcag cgtgggcagt
     3841 gcgatgtgca ccaggtccgg cttgctgctc gcgcgcgtcc agtagtcgtc ggcgtccttg
     3901 aagccgtgca gcggcgcggt caccaggttg tcgaactggt acaggtcgcg cgcgcgcgcc
     3961 accgcgtgcg cggcgtacag ccccgggtag cggcgcagct tggcgaggct cttgcgcttg
     4021 agcgacttga ggaagttcca ggtgtagacg cggttgaatc cgcggcccag cagccgtccg
     4081 gcgatcgtca gatccatcgg cgccgacacc gccgcggcgc gcgtcaccac ctgcgctgcg
     4141 gcttggcgct cgcgcgcgag ccatttcagc agggcgttac cgccgagcga gacgccgagc
     4201 gcgaaacgcg gcgcggcggg gctgattcgc gccgccgtac gcagcatcca gtcgatctcg
     4261 gcgtagtcgc cggaatgata agcgcgcgca aggcggttgg gcgcgcctcc gcagccgcgg
     4321 aagtgcggta ccacggcgcg ccagccgcgg acctgcgcgg cgcgcagcag cgcgcgcgcg
     4381 taatggctgc gcgagcagcc ttcgaggccg tggaacagca ccatcagcgg cgcttccgcg
     4441 cgcccttcca gccagtccac ttcgacgaag tcggcgtccg gtgtctccca gcgctcgcgc
     4501 cgcagcggcg gcagcggcag gcggcggaac aggtacggat agatggtctg cagatgcgcg
     4561 ccgggcagcc agcgcggcgc acggtagatc gccggcgcag ccatcctcgg acccgcgtcc
     4621 tcagtgaagc gtgcctaccg gcgcgtcggg tgcggcggcg tctggcggca gcggcgaggc
     4681 gtgatgcacc agcatgcgcc agcccgaagg cgtcagcacg tagacgttgg tggcgagcgc
     4741 aaggtgcgct ttgcgctgcc cgacgacgct gatgcgctcg tacaggttat gcaccgcgag
     4801 catgcgaccc gactgcacgc gcacgtccag cagtcgaaac cgcatgtgcg ggccgggcgc
     4861 gaacaacgcg cgccaggact cgcgtaccgc gtccatgccc atcaggcgct gtccgcccgg
     4921 gtggatgcac aggatctcct cgtcttcggc ccataccgcc atcatggcgc cgagatcggc
     4981 tttctcgaag gcgttgtaga aggcggcttc cgcctgctcg gagctggaca gcgtcatgcg
     5041 gcgcgcgcgc cgtcctcgag cacgtgtccc ttgagcacgt acaaggtgcc gcagtaggga
     5101 cacagcgcct cgccacgctg ctcgagcggc aggaacacgc gtggatgcgc gttccagagc
     5161 atgctctgcg gcgtcgggca gtgcagcggc aggtccgctc tatcgacctc gacccgtcgt
     5221 gcgccgtcgg tgctcttgcc ctgttccatg ccgtcggcgt ccgtcacacc ggcgtcagcc
     5281 agtgcacgta cttctggttg cgcccgttga ccacgtcgaa gaacatggac tgcaacttct
     5341 cggtgatcgg cccgcgcttg ccggcgccga tgacgcggcc gtcgagctcg cggatcggcg
     5401 tcacctccgc cgccgtgccg gtgaagaacg cctcgtccgc gatgtacacg tcgtcgcgcg
     5461 tgatgcgctt ggcctgcacc tccaggcctg catcgcgcgc cagctgagtc acggcgtcgc
     5521 gcgtgatgcc gatcagcgcc gaggtcagct ctggctcgta cagcttgccg tgcttcacga
     5581 tgaacaggtt ctcgcccggg ccttccgcca cgtacccgtc cacgtccagc agcagggcct
     5641 cgtcgtagcc gtgctcgacc gcttcctggt tggcgaggat ggagttggcg taggtgccgg
     5701 agaacttggc acggcacatg gtgacgttga tgtggtggcg cgcgtaggac gaggtcttca
     5761 cgcggatgcc tttctccagc ccttcctcgc ccagataagc gccccagggc cacgcggcga
     5821 tggccacatg caccctggcg cctttcggcg acacgcccat cttctccgag ccgtagaagg
     5881 agatcgggcg gatgtagcag gactcgagct tattggcgcg caccacctcg cgctgcgcgg
     5941 cgttcagggt ttcgcggtcg aacggcatct tcatgccgaa gatgtggcag gagttgaacc
     6001 agcgatcggt gtgctccttg aggcggaaga tggcggtgcc gccggcggtc ttgtaggcgc
     6061 gcacgccctc gaagcaggcg agcccgtagt gcagcgagtg ggtcaggacg tgcgtggtcg
     6121 cgtcgcgcca gggcacgagc ttgccgtcgt accagatcaa cccgtcacgg tctgccatcg
     6181 acatgtatct tgtctccctc gcaaaaacca gcattttagc gtagctttca atctgccagc
     6241 gccgattcgg ctttcaatct aacagcagcg gcgcggcgcg tgacgtcaca ggggtgcgct
     6301 ggacatgacc gtacagatcg aggtgatatc cgacgtggtg tgcccgtggt gcttcatcgg
     6361 caagcgccgg ctggagcagg cgctggcgcg gctgcgcgag gcgccgacgg cgcccacggt
     6421 gagcgtggcc tggctgccgt tccagctcaa cccggatctg ccggaaaccg gcatggaccg
     6481 ggccgattac ttgcggcgca agttcggtca cgccgcgccg cagatctatg cgcgcgtggc
     6541 gggggccggg cgctcggcgg gcatcgcctt tgcattcgat gccatcgcgc gtcagccgaa
     6601 cacggtggcc gcgcaccagt tgatcgccct ggccgcgccc gccgggcgcc aggaccagat
     6661 ggtggagacg ctgttccgcg cctatttcct ggaaggagcc gacctgacgc agcgcgcgac
     6721 gctggtcgcg ctggcgcagc gttccgggat cgatccggcg cgcgcaacgg cgtgcctgga
     6781 cgatccgctg caacgccagg cggtgctcga cgcggagcgc gacggccgcg agctgggggt
     6841 ggagggcgtg ccgttgttcc tgttcaatcg ccgtctggcc gtgtccggcg cgcacgagcc
     6901 gcaggtgctg ctggccgcca tcgaccaggc ggcccgggac gcggccgagg tgacgtagtg
     6961 cgcagccggc agggcgtgcg tggcccggcc gcgcgtgtgt tacaggcgag ccggcgcggc
     7021 cgttcggggt cgcggtgctg cgcgcgggct caggcacaag tcgtgctttg ggcagtgcgt
     7081 tcccagcctc acgcccccac ccaacccgaa taatacacgc catgccccag gtcaccgccg
     7141 ccgccgcctc cgtctccgtc agtgcggcac cggtcgccga ggagacgcgc gtgcttcagg
     7201 acaagacctc cgcggttccc gcttctgccg ccgccaggcg cgggtcgtgg ctggagcgcc
     7261 tgggccgcgc cgcggtgggc gaggacgacg acgacgagct gcggctgcgc aagacgctgc
     7321 tgatcttcgc cagcggcttg atgaacatgg cggccatcgt ctggctggcg atctactggg
     7381 cgctgggcct gaagctgccg accaccatcc cgctcgccta tcagctgctg tcggcgctga
     7441 tcctcgcggt ctatctcaag acgcgcaact tcgacttctt ccgcgtggcg cagctgtcgc
     7501 tgttcctgtt cgcaccgttc gtcatccagt ggagcatcgg cagcttcgtc agctccagcg
     7561 gcatcgtgct gctggcgctg ctcgccccgg tgggagcgat ggtggtgtac gggccgcgcg
     7621 agtcgattcc gtggttcgtc gcctatgtgg cgctcaccgc tctgtcgggc gcgttcgact
     7681 actacctggc cgacggcaat ctctccggcg tgccgatgcg cacggtggcg gtgttcttcg
     7741 tgctcaactt caccatcctg tcgagcatcg tctacctgct gctgcgctac ttcgtgcagc
     7801 agaaggacac cttccaggcc gaactggcgc gccagcacga gctggtgcag ctcgagcagc
     7861 gcaaggccga gcagctgctc accacgatcc tgccgccgca catcgcccag cgcctgaagc
     7921 aggatcagac caccattgct gacggcttcg ccgacgtttc ggtgatgttc gccgacatcg
     7981 tcaacttcac cacgctcgcc gaggagctga ccccgaagga ggtggtgagc ttcctgaacg
     8041 acgtgttcac ccgcttcgac gagctggcgg agaagcacgg cgtggacaag atcaagacca
     8101 tcggcgacgc ctacatggtg gcgggcggcg tatccggcga cgacacccag tacgtgtcct
     8161 cggtcgcgga cctggccctg gagatgcgcg atctgacgcg caacgacgag gtcatccgcc
     8221 gctacaacgt gtcgttccac gtcggcatcg ccaccggtcc ggtgatcgcc ggagtgatcg
     8281 gcgtcaagcg cttcatctac gatctgtggg gggacaccgt caacatcgcc agccgcgtca
     8341 cggcggaagc gccctcgggc gagatctacg tggacaagac cacctatcgg cgcctgggaa
     8401 cgcgctacac gttcagcgag ccgcaggagc tgatgttcaa gggcaaaggc aagatgaccg
     8461 tgtatcagct gctggggcgc agagcatcct agaagccgtg actcgtgact cgtgattcgt
     8521 agcacgagtg acgattcacg aatcacgagt cacgaatcac acagcgccct cgaacacggc
     8581 gcgccacagc gccagcaccg gccgcaccag ccccgcgacc ttgtccctct ccacccgtgc
     8641 gtaacgctcg ccttgcagcc gcagcgcgtg ctgcatgcgg cggaactcgc gatagctgtg
     8701 atgcaccgcc tcggccgcgt gggaatcgat caggcccagg cgaccggcca ggcgcagcag
     8761 cgccaggttg ccgatgttgc cggtgagctc ggcgtgtgtg tgggcgtgac ccagcaccag
     8821 atactggacg acgaactcca cgtccacgat gccgccgcga tcgtgcttga tgtcgaacag
     8881 cccgctcgtg ttcggatggc cttccagcat gcgcgcgcgc atcgccgcca cctcctcgcg
     8941 cagccgcggc agctctcgcg ggagccgcag gatctccacc cgcaactgct cgaagcgccg
     9001 cccgatgtcc gcgtcgccgg cgacgaagcg cgcccgggtc agggcctggt gctcgaacac
     9061 ccaggccttg ctgcgctggt attgcgcgaa ggcatccagc cggctcacca gcaacccgct
     9121 ggcgccgtcg ggccgcaggc gcaggtcggt gtcgtacagc acgccggccg ctgtgaagct
     9181 ggtgagccag gtgttcatgc gctgcgccag tcgcgcgtag ttctccggcg cgtccgggtg
     9241 cggatcgtgg tacaggaaga tgaggtcgag atcggatgcg tagcccagct ccttgccgcc
     9301 gagcttgccg tagcccacca ccgcgaaccg cgcatcgggc cgatggcgcg tgcgcaggtg
     9361 gctccaggcc aagcgcagca cctgctgcag gatcaggcag gcgaggtcgc tcaggtgatc
     9421 gctgagagtc tccagcggca gcgcgccggc cagatcctgg gtgagcaggc gcatcgtctg
     9481 cacctgcttg aaatggcgca gcagatccat ctgccgctcg atgccgtcgg ccgcgtccag
     9541 ctccgccgcc aggcgcgcgc ccagcgtcag ccaatccggg gcggagattg cgtcgggtgc
     9601 gatcaactcg tcgagcagca tcgggtggcg tgccaggtag tcggccgccc aggggctcgc
     9661 cgccgcaagg ccggccagcc gctgcaacgc cgccgggtac tcgagcaaga gcgccaggta
     9721 ggattcgcgc cggccgatgc tttccaccac gcgcgccagc cgctcgaggg tctgcccggg
     9781 gcgctcggag gccgcggccg cctcgagcag gcgcggcacc aatcggtcca cgcgcgcctg
     9841 ggtggacgcg gacatgcgcc ggtagagcgg cccgtcgtgg aactcgcgca ggcgcgccac
     9901 ggtcaccgcg gcgttctcgt atctcagcgc cgtcaagcgc gccagggcct ggtcctggtc
     9961 cagcgtgccg tgccacagcg cggacagcgg atgctccgcg acttcaccca ccgcaccggc
    10021 gaacaccgcc tcgaactggc gcgtgacgtt ggcgcgatgc cgctccagcg cttcgccgaa
    10081 ggccgctgcg tcggcgtagc ccatggcgcg cgcgatgagc gcgcggtcct ccgcgcttgc
    10141 cggcagcatc tgggtctgct gatcctccag gtactgcagc cggtgctcga ggttgcgcag
    10201 gaacacatag gccgcccgca gctcgtccac ggcctgcacc ggcagcaggc gccgttcgcc
    10261 gaggcgagcc agggcggcca gcgtaggccg cagccgcaag tccgcgtcgc gaccgccgcg
    10321 cacgatctgg aacacctgcg ccacgaactc gatctcgcga atgcccccgg gcccgagttt
    10381 gatgttgtcg tgcagctcgc ggcgctcgac ctccgtgcgg atctgcgcgt gcagctcgcg
    10441 cagcgaggcg aaggcgctga agtccaggtg gcgccggtag acgaacgggg tgacgagctg
    10501 cgtgagttcc ggcgccggtc ggccggtgat ctcgcgcgcc ttcacccagg cgtagcgctc
    10561 ccactcgcgg ccctgggtgt gcaagtagtt ctcgagcatg gcgaagctca tcgccaacgg
    10621 tccgctgtcg ccgtagggcc gcagccgcat gtccacccgg aacacgtggc cgtcagccgt
    10681 gtactgcgcc agcgcgttga tgagcttgcg tcccagccgt gtgaaatatt cgtgcgcgct
    10741 cagggggcgg ccgccgctgg tctggccctc ctcaggatag aggaagatca ggtcgatatc
    10801 ggaggagacg ttgagctcgg cgccgccgag cttgcccatg gcgacgacgt gcagatgctg
    10861 gaccgcgccg ctgtccgcgc cgaccggacg gccgtgggcc gcgtccaggg cgtcgtccag
    10921 atgcgacaaa gcgaagcgta cgctgtgctc ggccagcgcc gtggcggtat ccatcacttc
    10981 cgcgagatcc gccaggccgg tgaggtcgcg ggtgatcaga tgcagcatca ccgtgcggcg
    11041 cacctcgcgc agcgcgcgca gcaacgccgc ctcgtctgca gccggctggg cgtgcagcag
    11101 cgcgccgatg acggcgccgg tgagcggctg gtgcaggcgg gccgggtcga agctgcgcgg
    11161 cgggtgctcg cgcaggcggc gcaggaagcg gctgcactgg ccggcccggt ccaaaagctg
    11221 ctcggggtcc agaagctgct cgggcgctcc ctgctcgggt cgcggcggcg cggagggtga
    11281 ttgcatgggg attgggcggt agaatgggcg aacgaagcgc gctgtcctgt ggcggatggc
    11341 gatcgtcggc ggcgggtagc acgcccggcc gcgccggcgc cggttcggtc gcgcttcgcg
    11401 tcggggggcg cgccgccggg ttgctgcaag gaccggcagg cgtatgccgg gcagggcctg
    11461 cggccgaccg cgcgattcta ttttccgacc tgcacctctg gcaaatcccg agcccctggc
    11521 ccacttacgc cgtgacccgt ccatgacccg acgccccgct gtgcagctga tgcggcacgg
    11581 ctcgctgtgg ctgtaccggg cggtgatgct gaccctggtg ggcggcactg tgctggcggg
    11641 gggcgcagtg ctgttgctgc gctactgggt gctgcccggg gtcgacgcct atcgcccgca
    11701 gatcgaggcc gccttgagcc gcgcggccaa cgagcgcatc acggtgggag gcgtcgaggg
    11761 ccaatgggat ggcatccgcc cgcgcctcgc gctgcgcgag gtgcgcgtgt tcgaccgcgc
    11821 gggcgtcgag cgcctggccc tggccaaggt ggacggcacg ctttcctgga ccacactgct
    11881 ggccggcgag ccgcgcttcc actccatcga gttgaccggg ctctcagtcg aggtgcgtcg
    11941 cgaccgggcc ggcggttggc tgatcgccgg catgccgctc accatgcaag gcggcgacag
    12001 cggcttcggc gactggctgc aggagcagca ccgcattgcc gtgcgcgact cggctctcac
    12061 ctgggtcgac gaaacgctgg gcgggattgc gctgccgctg accgacgtcc agctcgaggt
    12121 ggaacggcgc ttcggcgcgc atcgtttcga cctgcgcgcg acgccgccgc tcgatgtggc
    12181 ggctcccctg ggattgcgcg gcgagctgcg gcatcccggc cgccgggcga gcggcaccct
    12241 atatctgcag gtcgggtacg cggacctggt cacgctgcgc cagtggctgc cgctgccggt
    12301 gtacgcagcg cgcggtgcgg gcgccctgga agtgtcgatg gacctcgagg agggcacgct
    12361 gcgctccgtc tccgcccagg tcgcgctgtc ggaggtgagc gtgcgcctgg acccggacct
    12421 gcctcctctg gagctgtcgc gcatgtccgg gcggctgggt tgggccggca atggccgcaa
    12481 gctcgaggtc ttggccagca atctcgcctt caccaccccg gacgggctgc atctgccgcc
    12541 ggccgacctg cgctaccgcc gctggggcgc cgcaggcgat ccggcgacgc gctccgagct
    12601 gcgcttcgac gcgctcgatc tggcagcggt gacgcgcctg gtcgaccgcc tgccgatcga
    12661 cgccggcgtg caggcgcgcc tgaccgaaat gaatccgcgc ggcaccctgc gcggcttcga
    12721 ggtcggctgg accgggccct ggggtggcca cggcgactat caggcgagcg gcgccttcga
    12781 gcgcgtatcg gtggccgcct cgggttatct tccgggcttc tcgtcggtca gcgggcagct
    12841 gcaggccgac cagcgcggcg gcagcatgag cctgcgggcc gaccagtcgg aactcgccat
    12901 gccgcacgtg ttcgtggagc cgttgccgct cgacagcctt gccggcacgc tcagctggtc
    12961 gatgcagtcg gacggtccgc tgctgcgcat cgagagcctg gtattcgcca atcagcatct
    13021 ggcggggaag gtcgccggca cctaccgcgc catcgccgga cagccgggcg ccgtggatct
    13081 gaccggctcg ctcgagcgcg gcgacggccg ccaggcatgg cgctacgttc ccctgcgggt
    13141 gcatgcgcgg gtgcgcgact ggctgcgcga gtccgtcgtg tccgggcaca cgcgcgatgt
    13201 gcagttccgg ctgcgcggcg atctcgctcg ctttcctttc gccgacggca agtccggcct
    13261 gttcgaggtg gtcacgcgct tcgaaggcgg cacgcttgct tacgcgccgg gctggcccgc
    13321 catcgagggc ctgcgcggcg agatgatgtt tcgtaacgcc ggcatgaacg tggtggcgga
    13381 ggcggcgcag gtgtacggga cgcggatggc gagcggtcgc gccaccattg ccgacctgag
    13441 cgtggacgac ccggtgctgg aagtgcgcat ggagaccgag ggcccgaccg aggactacct
    13501 gcgcttcgtc gagcacagcc cggtggaccg catgatcggc ggcttcagcc ggggcatgaa
    13561 ggccttcggg cgcggcgccc tggtgctggg gctgcagttg tccttgaagc atgcggagca
    13621 ggtgcgcgtc tcggggcgct accgcttcgc cgccaacacg ctggaccccg gccatggcgt
    13681 gccgccgctg gagcagctca gcgggcagct gtcgttcagc gagcgcgacg tgagcctgcg
    13741 cggcggcagc gcgcgcatcc tgggcatgcc cgcgcgcttc atcgccgacc gcggcggcaa
    13801 aggggcgctg gtgatccgcg gcgagggccg tgccgaggcc gccggcctgc gccaggtact
    13861 ggaccacccc ggggttgcgg cgctgggcgg cgcgaccggc tggcacagca cgctggcgat
    13921 ccaggacggc ggctatgacc tcgtcatcgc atccgatctg cgcggtctgt cctcgtccct
    13981 gccggccccg ttggccaagg acgccgcggc ggacctgtcg ttcaggctgc agcgccgctc
    14041 gatcaacgac cgccacgagc tggtcgtgct cactgtgggc gacatcgtct cggcgcagct
    14101 cctgcgcggc cgggatgcag cggccggcgt gctgcgcggg gaggtgcggc tcaacgcacc
    14161 ggcgccggcg cccgagcgcg acggcctgtg gctggcgggg aaactgaacg gcctcgatct
    14221 cgaccgctgg cggacgctcg tcgggcaggg cgcgtcggcg ctgccgattt cgctgtcggg
    14281 aatcgatctg cgggtggccg agctgcgtgc cttcggccgc gaatggcacg aggtggcgct
    14341 gaaggtgagc accacaccgg cccggtggga aggcagcctg gtctcgtggg aagccgcggg
    14401 caccctgttc tggacgccgg cggggcccgg tgcgctgacc ggccggttca cacgcctgca
    14461 cctgcccccc gcgccgtccg agctgcgtgc cgcgcaggcc ggcggcggtg cgggctatcc
    14521 ggcgctcgac gtgacggcgg aggacttccg cctcggcgag agccagctgg gacggctgac
    14581 gctggtggcg accccggaga acggcgaatg gcgcatccgc cagctcgacc tgcgcagccc
    14641 cgaggggacg gtgagcatga agggggcgtg gcaggctctc ccggcgccgc tgacgcgcct
    14701 ggacatcaag ggcgaggtga acgacatcgg cggctacctc gcgcgcctgc agctgccgaa
    14761 gggaatcgac ggcggcagcg cccgggtaca gggccagctg tcctggagcg gatcgcccca
    14821 ggcggtggac tggccgacgc tgtccggcgc actgcagctg gaggcgaaaa aaggccagtt
    14881 tctgaaagtc gagccgggta tcggcaagct gatcggcgtg atcagcctgc agtcgctgcc
    14941 gcggcgggtg gcgctcgact tccgcgacgt gttcagcgac ggcttcgctt tcgatgagat
    15001 ctcggcaagc gcggccatcg cccgcggcgt cgcgcgtact caggacttca gcatggcggg
    15061 tccgtccgcc cgggtggaga tgaaaggcga gatcaacctg gcgggcgaga cgcagcgtct
    15121 cgacgtcaag gtggtgccct cggtgagcga gagcgtcgcg ctcggtgccg ccatcgtcaa
    15181 tccggccgtg ggcatcgcca ccctgctggc gcagaaggcg ttgaaggacc cgatcggcgc
    15241 gatggtcgcg ttcgaatacg aggtgagcgg tacctgggac gatcccatgg tgatgaagaa
    15301 gaaacgcgag ccggcgcggc cgcagccgca gggacgcaag tagcgacccg ccgcggaaca
    15361 tgagaccggc gccgccgcgg ccgtccgttt cgccccttga acagtagcgt gccgtcacca
    15421 actgatctgt tggcgcccgc gcgctagact caaactttgt tccccgttcg acagcagccg
    15481 atacgatgac cgccgtcacc ccctccgaag tcctgttcgc caccgcccac cagaccctgc
    15541 tcgcgcccta tggactggac gcgggccggc tcacgcgcgt cttcggcgag atcatgaccc
    15601 accagatcga ctacgccgac ttgtatttcc agtacagccg cggggagagc tggagcctgg
    15661 aggagggtat cgtcaagtct ggcagcttca gcatcgagca gggcgtgggc gtgcgcgccg
    15721 tgagcggcga caagaccgcg ttcgcctatt ccgacgacat cagcctgccg gcgctgcagt
    15781 cggcggcggc ggccacccgc gccatcgcgc gccagggcgg agaagcgcgc accgcggtgg
    15841 cgcgccgcag ccacggacgc gagctgtacc tgccgcacga ccccatcgcc agcctcgacg
    15901 cgccgcgcaa ggtggcgctg ctggagcggc tggaaggctt cgcgcgcgct cgcgacccgc
    15961 gcgtcaagca ggtgatggcc agcctcacag gcgagtacga ggtggtgctg gtggcgcgct
    16021 ccgatggagt gctcgccgcc gacgtgcgtc cgctggtgcg cctgtccctg caggtgatcg
    16081 ccgaggaggg cgaccgccgc gagcaaggca gtgccggcgg gggcgggcgg ttcgactacg
    16141 tctatttcga cgacgagatg ctgcgcggct acgcggaaaa agccgtggat caagccctga
    16201 tcaacctgcg ggcgcgccca gcgcctgccg gcacgatgac ggtggtgctg gggccgggct
    16261 ggcccggcgt gctgctgcac gaggcggtgg gtcacgggtt ggagggcgac ttcaaccgca
    16321 agggcacctc cgctttcagc ggccgcatcg gcgagcgcgt cgccgcgccg ggcgtgaccg
    16381 tggtggacga cggcacgctg ccgcgccggc gtggctcgct caacgtcgac gacgagggca
    16441 atgcgacgca gtccacggtg ctgatcgagg acggcgtgct gaccggctac ctccaggacg
    16501 cgctgaatgc gcgcctgatg gggatggcgc ccaccggcaa cgggcggcgc gagtcgttcg
    16561 cgcacgtgcc catgccgcgc atgaccaaca cctacatgct ggggggcgcc agggatccgc
    16621 aggagatcat cggctcggtc aagcgcggcc tgtacgcggt gaacttcggc ggagggcagg
    16681 tggacatcgt cagcggcaag ttcgtgttct ccgcctcgga ggcctacctg atcgaggacg
    16741 gcaaggtcgg cgatccggtg aagggcgcca cgctcatcgg caacggaccg gacgcgctca
    16801 cccgcgtcgg catgatcggc aacgacatgt cgctcgacac cggggtcggc acctgcggca
    16861 aggagggcca gagcgtgccg gtgggcgtgg gccagccgac gctgcgcatc gacgggttga
    16921 cggtgggcgg gacgcagtct tgatcctgat cctcaacgag aacgccgacc ccgcgggacc
    16981 cgagtacgcg gcgctgatgg aggcgctgcg gcagctgccg ggcatccagg tcaaggcgca
    17041 ccgcatcgaa ggcgagctgc gcgcggtcac cgagatctac ctgatcggcg acaccaagca
    17101 gctctcgccg gaggacatgc gcgccttgcc gctggtcgag cgcgtggtgc gcgtgtcgga
    17161 agagtaccgt gtgctcggcc gtcacaagga cgacgtgcgg ccgacctggt tcgagtacca
    17221 gggactgcgc ttcgggcagg atacgctgca cctgttcgcc gggctgtgcg ccgtggacac
    17281 gcgcgagcac gtggagcgga tgtgccgcgc cctgcgcgac aacggccagg tgtgcacccg
    17341 catgggggcc tacaagccgc gcaccagccc gtatgcgttc cagggccacg gtcgcgcctg
    17401 cctcaactac gtcttcgaga tcgccggtaa gtacggcatc cgggtcgtcg ccatggaggt
    17461 cacccacgaa gcgcaggtgg acgagatccg cgacgcgctg cgcgagaccg aaagcccgac
    17521 cggcgcgatg ttgcagatcg gcacgcgcaa cacgcagaac ttcgagctgc tgaaggcggt
    17581 gggccggcag cggcagtttc cggtgctgat caagcgcggc ttcggcatca cgctggacga
    17641 gtccctgaac gccgcggagt acctggcaag cgagggcaat caacgcgtga tcttcgggct
    17701 gcgcggcatg aagacgcaca tgggcgaccc gcaccgcaac ttcgtggact tcgtgcacgt
    17761 gccggtggtc aagcggctga cccgcatgcc ggtgtgcatc gatccgtcgc actcggtggg
    17821 ctcgcgcgcc cgcggggtcg acggcgtgct cgacctgatg catgccaccg cccagggggt
    17881 gatcgccggc gcgaacatgg tcctggtcga tttccatccc gagccttcca aggccctggt
    17941 ggacggcccg caggcgctgc tgctctccga gctgccgcag ttcgtcgagg acgtcgccat
    18001 cgcgcgcgag gcctacctga aacggaccga gctggcccgt cgcaaggccc tctaggcctg
    18061 gtgaactatc cgggctagca tcccggccgg tccgctgtct gcatcccgcg gccccgccgg
    18121 ctttgccaaa gccgggcgca tgctcgataa tttagccctc gcccgcccgc tgcgcgtcgc
    18181 gccgcaacca cgatgatcga acccgccgcg caatactccc tgctcaagga cctggaacag
    18241 ctcggtgaag gcaccgccct cgccgagcag atctacagca tgatcgggca ctcgcgcttc
    18301 ttcgacgact tcagccgcga ggacgtgcag gagctggcgg gcttcatgca ggtgtaccgc
    18361 gccgagccgg gcgcgatgat catccgcgag ggcgacgtgg acgactacat gctgctcatc
    18421 atccagggca aggtggacat cgtgaagacc gacaagcgcg gcgtcatcca ggccatgacc
    18481 agcgtcggcc cgggcatgac cctgggggag atgtcgatga tcgacggcga gccgcgcttc
    18541 gccacctgca tcgcgatcga gcccaccacc ttcgccgtgc tgtcgcgcga cgacatggtg
    18601 cgcatcatcc tggaagagcc gagcctgggc gcgaagctgc tgatcaagct ggtcacgctg
    18661 ctgtcgcaac ggctgcgcca gaccagctcc acgctgctgc actacatgga gcgttgaccg
    18721 accccgtcgc ctcgcgccgc cgttttgcgg ccctgctggc gctggccgca gcagcgccct
    18781 gcgcgctggc tcgatccgcg cccgtggccg acgaccgcgg caaggtgctg cggctgcaat
    18841 tccccgcacg gcgcatcgcc gcgctctcgc cccatctggc cgagctcacc ttcgacgccg
    18901 gcgccggcgg cgcgctgatc gccgcggtgc gcggcagcga ctacccgccc gaggtgaagc
    18961 gcctgccgac gatcggcgat gcggcagggc tcgacctgga acgcatcctg gccttgcgcc
    19021 cggacctcgt gctgggctgg ctgtcgggca accgcgcgac cgacatcgag cggctcgaga
    19081 ggctggggct gccggtcttc gtcagcgaac cgcggcgcct gagcgacgtg ccggcgacgc
    19141 tgcgccgcat gggtacgctg gccggcacgc gcgcagccgc cgagcacgcc gcgcgggctt
    19201 tcgaggccag gctcgcacgc ctgacgccac cgtccgtctc gcctccggtg agcgtgttcg
    19261 tcgagatctg gcacaccccg atgatgacgg tgaacggcgc gcatctgatc agcgaagtgc
    19321 tggccgcctg cggcggacac aacgtcttcg ccgatctgcc ggcgttggcc gccagcgtcg
    19381 gcgtggaggc ggtcctggcc gccgatccgc aggtgatcat cgccaccacg cctcccggac
    19441 aggacgcgct cgccgcatgg caggccctgc cgcggctgcg cgcggtacgc gccgggcgct
    19501 tgcacgccat cgacccggat ctgctcacgc gcgccacgcc gaggatcctg gacggggtcg
    19561 agcaggtatg cgcgtggttg cggccgtgac cgctcacgcg gctacagctt cgtgcactgg
    19621 cccttgtaca acaccggtcc cgtgggcagc ccggtgggcg atccgccctt gggctccacg
    19681 ctcattgcca ggccccaggc ggcgttgatc gccgtggcca gctcccgcgg caccagcacg
    19741 ctctgggtct cgtgggtggt gatcaggccc agggacagcg gcttgcgatc gccgcccggg
    19801 agcatccaca actcccaggc cgtgtccggc gccatctcgg catgtcccag cacccggatg
    19861 cgcatgcgct tgtcgccgcg gccgtcggtt tcccaggaaa ccgtcatcct cggttccgcg
    19921 cgctcgtccg acatcaccac caccatcagc tccgcgcgct ccggctgcag caccccgagg
    19981 cttgccgtcg atatcagcgc cgccgccagc gcggcgaagg cggctccgcg ccagaaccca
    20041 aggctgtccc acagcgccgc ccacccgcgc ggagcggcgg tcacgcgtcg ctggatcgca
    20101 cgccacacgc gcgccggcgg ctgcaccggc gcgactgtgg cgtcgagcgg agccaggcgc
    20161 tcctgccagc tcgtcacgta gcggcgcagc gccgggtcgt ccttgagcga gcgctcgaag
    20221 cgccggcgcg cgcggccctg cagggtgccg agcgcatact cggcggccag ggcttcccgc
    20281 accagctcgt tgcggtactt catgtgcgct ctagtggtcg tccagacagc tcttcagctt
    20341 ttccagcccg cgccgaatcc aggtcttcac cgttcccagc ggctgtcgca ggtgccgggc
    20401 cagttccccg tgggacaggc cgtgcgcata ggcgagcgcg atgctctgcc gctggttgct
    20461 gctcagccgc ttcaggcatt ccgccagggc ccgcgccgcg cgctcgctct gcagcgcctg
    20521 ctccgggccc gggcggtcat cggccagcgc ttcgaccagc aggtcgtaat cctcgctgcc
    20581 gtgctcctga cggggccggc gcagccagtc cagcgcgcgg ttgcgcacga tcgccgtcat
    20641 ccaggtcatc ggcgcgctct tctgcgcgct gtattcgccg acatggttcc aaatgttaac
    20701 gaagctctcc tgcagcactt cctccgccca gtcctccctg cgcaatatac gcagcgccac
    20761 cgcgtacagt ttcgcggcgg tgtggcggta gagctgagcg aaggcctgcg cgtcgcgcag
    20821 cgcgcaacgg cccatgagct cgccgatggg atcgtaatca ggcatccgct gcgcgcgccg
    20881 aggtcctgcc gcggcgccgc gccgtcgcgc gcgccatcat ccgggttttc cgggacccgt
    20941 gtccttgcgc cacggcacgt ccggctgccc ggcgctgcgg ttgagcacgc ggctcaggac
    21001 gaacagcagg tccgagagac ggttgaggta ctgctggcaa tacgccgaca gcgtctctgc
    21061 ggatgcgagg ctcacgactc tgcgctcggc ccgccggcac acggcgcgcg ccaggtggca
    21121 cagcgcggcg ctgcggctgc ctccgggcag gatgaattcc ttgagcggtt ccagcccggc
    21181 attgtgcgca tcgagcagcg cctcgagctg ctgcacgtgc gcgccggcga tgcgggtgta
    21241 gccgggaatc gaaagctcgc cgccgaggtc gaacagatcg tgctgaatgg cggtgagctg
    21301 gccgcgtacg tcctcgggca gcggctcggc cagcagcacg ccgatcgcgc tgttcagctc
    21361 gtccaccgcg ccgagcgcct cgacgcgcac gctgtccttc tgcacgcgcg tgccgtcgcc
    21421 caacccggtg ctgccgccgt ctccggtgcg cgtgtagatc ctggtgagtc gatggcccat
    21481 gtggcctcaa gccggcggaa gtcgacagcg ccggattata ggcccgcggc ggggcagggc
    21541 caaattgacg tgccgcccgg ccgtggtcgc tcggatggtg gcgcccgcgg tgcgcgatcg
    21601 cagccgcggc attgacccgc tgcagacctt cctttacagt tcgcgagttt tttcctgccc
    21661 gctgaccgcc ccgttcgacg acccaccatg acccgctccc gagcgttagc ggccgcggcg
    21721 gctgccgccg ccctgagcct cggccagtcg ctttcggctg cgcaaggtga cccgctcgca
    21781 gccgtccaca agaagcagat gtgcgagggc tgtcacggca aggaaggcta tcgcaccgcc
    21841 tatcccaccg tgtacagcgc gccgaagctg ggcggcca
//
LOCUS       DASPGE010000150         7462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_745779, whole genome shotgun sequence.
ACCESSION   DASPGE010000150 DASPGE010000000
VERSION     DASPGE010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745779"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="VD791_04775"
     CDS             complement(<1..1243)
                     /locus_tag="VD791_04775"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXV07318.1"
                     /translation="MAEAAVRPAPLSAAQRAAAARELSWRRALALSARLLGRDWRAGE
                     LRVLAVGLIVAVASLTTVAFFADRVKQALSAEANHLLGADLMLISDRPIAPSFAERAR
                     ALGLATVDLVRFPSMTVAGESTVLTEIKAVGPGYPLKGRIAIRTEAQAEPVRPQGIPE
                     PGTVWVDDRLLGRLGVAVGDALGVGERRFRIAAQVVEDPETSVGFLNLGPRLTLNLAD
                     LPSTGLIAVGSRVSYRLGVAGEPAAVAAFRVQAQRGIGPGQRVEDVRDARPEIRSALE
                     RAEKFLGLSALLSVILAAVAVALAARRYLRRHLDGVAIMRCLGASQGLILRLHVAQFV
                     VLGLLASALGCMLGVAGQSVLAALLTPLVGVALPLPGWMPVLQGFAAGFVLLLGFALP
                     PIVALRRVPTLRVLRRDLGLPD"
     gene            complement(1236..1907)
                     /locus_tag="VD791_04780"
     CDS             complement(1236..1907)
                     /locus_tag="VD791_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV07319.1"
                     /translation="MEPIVRARGLTKQVDTGRNRLTILQDVNLEIAPSEAVAILGASG
                     SGKTTLLGLLAGLDMPTSGDVILDGVDLARLDEDGRAALRRKLVGFVFQSFQLLPALT
                     ALENVMLPLELDGAPDARESATALLARVGLAERLDHYPRQLSGGEQQRVAIARAFAGT
                     PRLLFADEPTGNLDSATGDEVIHLMFDLNREQRTTLLLVTHDERLAGRCGRRLRLAAG
                     RMVDG"
     gene            1906..2511
                     /locus_tag="VD791_04785"
     CDS             1906..2511
                     /locus_tag="VD791_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HXV07320.1"
                     /translation="MLVRFLLLTLWLAAAPLQAQPPVILVLGDSLAAGYGLPQGRGWV
                     DLLQDKLAAEGLDHRVVNASISGETTLGGRNRIAAALERHRPDIVILELGANDGLRGQ
                     PLAALRDNLTAMVRAGRRSGAQVLLIGMRIPPNYGQDYTRKFHAAFTDVARAERVPLV
                     PFLLEGFADKRELFQPDGVHPNEQAQPRIVATVWKALRPLL"
     gene            2596..3672
                     /gene="mnmH"
                     /locus_tag="VD791_04790"
     CDS             2596..3672
                     /gene="mnmH"
                     /locus_tag="VD791_04790"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HXV07321.1"
                     /translation="MSDQPPPRDRATVAQLLEFDAVIDVRTPSEFALDHIPGASSLPV
                     LSDEERARVGTLHKQMSPFAARKVGAALISASIARHLESALRDRPRAWRPLVYCWRGG
                     KRSAALTHVLREIGWDAQQLDGGYKAFRRAVMADLPALAPRLGYRVVCGFTGSGKSRL
                     LQALRAAGAQVLDLEALAAHRGSVLGNLPGAPQPTQKMFESLLWDRLRRFDRQRPVFV
                     ESESKKIGLLQVPQTLLDCMWAGDCVQLSTPRPLRVELLMQEYAHFLSDPDLLGKQLD
                     CLVPLHGHAVIERWKAFAAAGEWEALVDELLVRHYDPAYSRSIVSHYPRLPQARVVQL
                     EGGGEAAFSRAASRLLDEAPEAVT"
     gene            3669..4661
                     /locus_tag="VD791_04795"
     CDS             3669..4661
                     /locus_tag="VD791_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXV07322.1"
                     /translation="MNIGLKIDVNTYRGTRRGVPALVALLQKHRAGASFLFNLGPDRT
                     GRAVGRLLRPGFLGKAWRTSALSHYGPITLLRGTLLPSVDIGRRCAGLMRSVRDAGFE
                     VGIHTYDHLRWENRVAHAGAAWTRRELERACQRFRDIFGTEPRVHGAAGWRMNRHAYR
                     LTQALGFGYCSDTRGTQPFVPLCQAEVIACPQLPTTLPTLDELIGTDGITRDNVAEQI
                     LQRTRDGLDTGHVYTAQAELEGMKLAPAFERLLQGWREMGCRVLSLGDYFDALPDKNL
                     PRHEVAIGTVPGRAEYVALQGEEFLAWPAPTTPLPEGGRLPSPPAPLPQRGEGS"
     gene            4675..5133
                     /locus_tag="VD791_04800"
     CDS             4675..5133
                     /locus_tag="VD791_04800"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXV07323.1"
                     /translation="MLENLPVPDFAAPATGGRTVDRAGLRGRPFVLYFYPKDNTPGCT
                     DEGMQFRELHPQFAKAGCGIYGVSRDTLKSHDGFKAKMSFPFDLISDPDERLCEMFGV
                     IKMKNMYGKQVRGIERSTFVVDGNGVVRREWRGVKVPGHAQEVLDFVRSL"
     gene            5114..6640
                     /locus_tag="VD791_04805"
     CDS             5114..6640
                     /locus_tag="VD791_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HXV07324.1"
                     /translation="MSSARSRPRHAHQSMPRCATHRPLLSCSLILEPRSPMSRKPAQV
                     TRLHHDSKLFVLDTNVLMHDPTSLFRFEEHDIFLPMATIEELDANKKGMSEVARNARQ
                     VSRFLDAMMRDGEAQIEDGIPLEQQGDKAAHGRLFLQTQAIAGELPATLASGRADNEI
                     LGVVRHLQELYPRRHVILVSKDINMRIKARALGLAAEDYYNDKALEDTDLLYTGVRAL
                     PADFWDTHGKEMESWQQAGRTWYRVHGPLVPSLLVNEFAYLETDRPFYAQVKEVSGKT
                     AILQTLTDYGHQRNSVWGIVARNREQNFALNLLMNPEVDFVTLLGQAGTGKTLLTLAA
                     GLMQTLEDKLYSEIIMTRVTVPVGEDIGFLPGTEEEKMTPWMGALEDNLDVLNKTDED
                     AGEWGRAATRDLIRSRIKVKSLNFMRGRTFINKFLILDEAQNLTPKQMKTMITRAGPG
                     TKVVCLGNIAQIDTPYLTEGSSGLTYVVDRFKGWSHSGHVTLQRGERSRLADHAAEIL
                     "
     gene            6667..>7462
                     /locus_tag="VD791_04810"
     CDS             6667..>7462
                     /locus_tag="VD791_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXV07325.1"
                     /translation="MPFADSAGVRLHYEENGSGTPVIFVHEFAADCRSWEPQVRYFAR
                     RYRCITFNARGYPPSDVPRDPEAYSQRHAVDDIAAVLHHLKIDRAHICGLSQGGYATL
                     HFGIHYPRMALSLAICGAGHGSDPGTRAQFLKDGAELAERIERLGMEEGIKHYAASPT
                     RLPLRHKDPRGYEEFYRQFAEHSNIGSALTMRGYQLRRPTIYELERDIEKIDVPTLIV
                     TGDEDGPCLEPGIFMKRKIRSAGLLVIPNSGHSINLEEVDAFNRALL"
BASE COUNT         1236 a         2647 c         2515 g         1064 t
ORIGIN      
        1 tgtccggcag tccgaggtcg cgccgcagca cccgcagcgt cgggacgcgg cgcagcgcga
       61 cgatcggcgg cagcgcgaaa cccagcagca ggacgaagcc ggccgcgaag ccctgcagca
      121 ccggcatcca tcccggcagc ggcagcgcca cgccgaccag cggcgtgagc agcgccgcca
      181 gcaccgattg gccggccact cccagcatgc aacccagcgc gctcgcgagc agacccagca
      241 ccacgaattg cgccacatgc aggcgcagga tcagcccctg gctcgcgccc aggcagcgca
      301 tgatcgccac cccgtccagg tgccggcgca ggtagcggcg cgccgccagg gccaccgcga
      361 ccgcggccag gatcacgctg agcagcgcgg acagcccgag gaatttctcg gcacgctcca
      421 gcgccgagcg gatctcgggg cgcgcgtcgc gcacgtcctc gacccgctgc cccggcccga
      481 taccgcgttg cgcctgcacg cgaaacgcgg ccaccgcagc cggctctccg gccacgccca
      541 ggcgatagct cacgcggctg ccgacggcga tcagcccggt ggatggcaga tcggcgagat
      601 tgagcgtcag gcgcgggccg aggttgagga agccgacgct ggtctccgga tcctccacca
      661 cctgggcggc gatgcgaaag cgcctctcgc ccacgcccag cgcatcgccg accgccacgc
      721 cgaggcgccc gagcagccgg tcgtccaccc agacggtgcc gggctcggga atgccctggg
      781 gccgcacagg ctcggcctgc gcctccgtgc gaatggcgat gcgccccttc agcggatagc
      841 ccggcccgac ggctttgatc tcggtgagca ccgtgctctc ccccgccacc gtcatgctcg
      901 ggaagcgcac caggtccacc gtcgccagac ccagcgcgcg cgctctctcg gcgaaggacg
      961 gggcgatcgg gcgatcggag atcagcatca gatccgcacc cagcaggtga ttggcctccg
     1021 cgctcaaggc ctgcttcacc cggtccgcga agaaggcgac cgtggtcagg ctggccacgg
     1081 ccacgatgag gcccaccgcg agcacgcgca gctcgcccgc gcgccagtcg cgccccagca
     1141 agcgcgccga caaggctagc gcccggcgcc acgagagctc gcgcgccgca gccgcccgct
     1201 gtgccgcgct gaggggtgca ggccgcactg cggcttcagc catcgaccat ccttcccgcg
     1261 gccaggcgca gacggcggcc acagcggccc gccaggcgct cgtcatgggt caccagcaac
     1321 agcgtggtgc gctgctcgcg gttgagatcg aacatcaggt ggatcacctc atcgccggtg
     1381 gccgagtcca ggttgccggt cggctcgtcg gcgaacagca gccgcggcgt gccggcgaac
     1441 gcgcgcgcga tcgcgacgcg ctgttgctcg ccgccggaga gctgacgcgg gtagtggtcc
     1501 agccgctcgg cgagcccaac gcgcgcgagc agcgcggtcg cgctttcgcg cgcgtccggc
     1561 gcaccgtcca gctccagcgg cagcatcacg ttctcgagcg cggtgagcgc gggcaggagc
     1621 tggaacgatt ggaacacgaa gcccaccagc ttgcggcgca gcgccgcgcg cccgtcttcg
     1681 tccagccgcg cgagatcgac gccgtcgagg atcacgtcgc cggaggtggg catgtccaat
     1741 ccggccagca ggccgagcag tgtggtcttg ccggaaccgg atgcgcccag gatggcgacg
     1801 gcttcgctcg gcgcgatctc caggttcacg tcctgcagga tggtcaggcg gttgcggccg
     1861 gtgtcgactt gcttggtcaa accccgggcc cgcacaatag gttccatgct cgtcagattc
     1921 ctgttgttga cgttgtggtt ggcggccgcg ccgttgcagg cgcaaccgcc ggtgatcctg
     1981 gttctcggcg acagcctcgc ggcgggctac ggcctgccgc aggggcgcgg gtgggtcgat
     2041 ctgctgcagg acaagctcgc cgcagaaggg ctcgaccatc gcgtggtgaa cgccagcatc
     2101 agcggcgaga ccacgctcgg cggccgcaac cgcatcgccg ccgcgctcga gcgacatcgg
     2161 cccgacatcg tgatcctcga gctgggcgcc aacgacggcc tgcgcggcca gccgctcgcg
     2221 gcgctgcgcg acaacctgac ggccatggtc cgggccggcc gccgcagcgg cgcgcaggtg
     2281 ctgctcatcg gcatgcgcat tccaccgaac tacggccagg attacacgcg gaagttccac
     2341 gccgccttca ccgatgtggc gcgcgccgag cgcgtaccgc tggtgccctt cctgctggaa
     2401 ggctttgccg acaagcgcga actgttccag ccggacggcg tccaccccaa cgagcaggct
     2461 cagccgcgca tcgtggccac cgtgtggaag gcgctgcggc cgctgctata gtggactgcg
     2521 gcccgcctca gcccgccggc caggtgtgtt acccttcgcc tcgccccgag atgtgccagg
     2581 ggatatggca gcgacgtgag cgaccaaccc ccgccccgcg accgggcgac cgtagcacaa
     2641 ttgcttgagt tcgatgcagt catcgacgtg cgtacgccgt cggagttcgc gctcgaccat
     2701 atcccgggcg catccagcct gccggtgctg tccgacgagg agcgcgcgcg cgtaggcacg
     2761 ctgcacaagc agatgtcgcc gttcgccgcg aggaaggtgg gcgcggcgtt gatctcggcc
     2821 agtatcgcgc gacacctgga gagcgcgctg cgggatcgac cgcgagcctg gcggccgctg
     2881 gtgtactgct ggcgcggggg caagcgcagc gcggcgctca cccacgtcct gcgcgagatc
     2941 ggctgggacg cgcagcagct cgacggcggc tacaaggcgt tccggcgcgc cgtgatggcc
     3001 gacctgccgg cgctggcgcc caggttgggc taccgcgtgg tatgcgggtt caccggctcg
     3061 ggcaagagcc gcctgctgca ggcgttgcgc gccgccggcg cgcaggtgct cgacctggaa
     3121 gcgctggccg cgcatcgcgg ctcggtgctc ggcaaccttc ccggcgcgcc gcagccgacc
     3181 cagaagatgt tcgagagcct gctgtgggat cgcctgcggc gcttcgaccg ccagcgcccg
     3241 gtgttcgtcg agtccgagag caagaagatc gggttgctcc aggttccgca aaccctgctc
     3301 gattgcatgt gggccggcga ttgcgtgcag ctctccacgc cgcgcccgct gcgtgtggag
     3361 ctgctgatgc aggagtacgc gcatttcctc tcggatcccg atctgctcgg caagcagctc
     3421 gactgcctgg tgccgctgca cggtcacgcg gtgatcgagc gctggaaggc gttcgccgca
     3481 gcgggcgagt gggaagccct ggtggacgag ctgctggtgc gccactacga tcccgcctac
     3541 agccgctcga tcgtttcgca ctatccgcgc ctgccgcagg cgcgcgtcgt gcaactggag
     3601 gggggcggcg aggcggcgtt ctctcgcgcc gcatcccggc tgctggacga agcgccggag
     3661 gcggtgacgt gaacatcggg ctgaaaatcg acgtcaacac ctaccgcggc acgcgcaggg
     3721 gcgtgccggc gctcgtggcg ctgctgcaga agcaccgggc cggcgccagc tttctgttca
     3781 acctggggcc cgatcggacc ggccgcgcgg tcggacgcct gttgcgcccg gggttcctgg
     3841 gcaaggcctg gcgcacctcg gcgctgtcgc actacgggcc gatcacgctg ttgcgcggga
     3901 ccttgctgcc gagcgtggac atcggccggc gctgcgccgg cctgatgcgc tcggtgcgcg
     3961 atgcgggctt cgaggtcggc atccacacct acgatcacct gcgctgggag aaccgcgtcg
     4021 cgcacgccgg cgccgcctgg acccggcgcg agctcgagcg cgcctgccag cgcttccgcg
     4081 acatcttcgg caccgagccg cgcgtgcacg gtgccgccgg ctggcgcatg aaccgtcacg
     4141 cctaccgtct gacgcaggcg ctgggcttcg ggtactgctc ggatacgcgc ggcacgcagc
     4201 cgttcgtgcc gctgtgccag gccgaggtca tcgcctgccc gcagctgccg accacgctgc
     4261 ccaccctgga cgagctgatc ggcaccgacg gcatcacccg cgacaacgtc gccgagcaga
     4321 ttctgcaacg cacccgggac ggcctcgaca ccggccacgt ctatactgcc caggccgagc
     4381 tggagggcat gaagctcgcg ccggcgttcg agcggctgct gcaaggctgg cgcgagatgg
     4441 gctgccgggt gctatccctg ggcgactact tcgacgcact gcccgacaag aacctgccgc
     4501 gccacgaagt cgccatcggg acggttcccg gacgcgccga gtacgtggcg ctgcagggcg
     4561 aagaattcct cgcctggccc gcacccacaa cgcctctccc cgaggggggg cggctcccct
     4621 cacccccggc ccccctcccg cagcggggag agggcagcta agaaggagca agccgtgctc
     4681 gaaaatctcc cggtccccga cttcgcggcg ccggccaccg gcggaaggac ggtcgaccgc
     4741 gccgggctgc gcggccggcc cttcgtgctg tacttctatc ccaaggacaa cacgccgggc
     4801 tgcaccgacg aaggcatgca gttccgcgag ctgcatccgc aattcgcgaa agccggctgc
     4861 ggcatctacg gggtgtcgcg cgacaccctg aaatcgcacg acggcttcaa ggccaagatg
     4921 agcttcccgt tcgatctgat ctccgacccc gacgagcgcc tgtgcgagat gttcggcgtg
     4981 atcaagatga agaacatgta cggcaagcag gtgcgcggca tcgagcgcag caccttcgtc
     5041 gtcgacggca acggcgtggt ccgcagagag tggcgcgggg tcaaggtccc gggtcacgct
     5101 caggaagtcc tcgatttcgt ccgctcgctc tagaccgcgc cacgcgcacc agtcgatgcc
     5161 tcggtgcgca acacaccgcc cgttgctttc ctgttccctg attctcgagc cccgatcccc
     5221 gatgtcccgc aaacccgcgc aagtcacccg cctgcatcac gacagcaagc tgttcgtgct
     5281 ggacaccaac gtgctgatgc acgaccccac cagcctgttc cgcttcgaag agcacgacat
     5341 cttcctgccg atggcgacca tcgaggagct ggacgccaac aagaagggca tgtccgaggt
     5401 ggcgcgcaac gcgcgccagg tgagccgctt tctcgacgcc atgatgcggg acggcgaagc
     5461 gcagatcgag gacggcatcc cgctcgagca gcagggcgac aaggcggcgc acggacgcct
     5521 gttcctgcaa acccaggcga tcgccggcga gcttcccgcc acgctggcga gcggcagggc
     5581 cgacaacgag atcctggggg tcgtgcgcca cctgcaggag ctctatccgc ggcgccatgt
     5641 gatcctggtg tccaaggaca tcaacatgcg catcaaggcg cgcgcgctgg gtctggccgc
     5701 cgaggattac tacaacgaca aggcgctgga agacaccgat ctgctgtata ccggcgtgcg
     5761 cgcgcttccc gccgacttct gggacacgca cggcaaggag atggaatcct ggcagcaggc
     5821 cggccgtacc tggtaccggg tgcacgggcc gctggtgccg agcctgctgg tgaacgagtt
     5881 cgcctacctg gagaccgacc gtccgttcta cgcgcaggtc aaggaggtga gcggcaagac
     5941 cgccattctg cagacgctca ccgactacgg ccatcagcgc aacagcgtat gggggatcgt
     6001 ggcgcgcaac cgcgagcaga acttcgcgct caacctgctg atgaaccccg aggtcgattt
     6061 cgtcaccctg ctgggtcagg ccggcaccgg caagaccctg ctcacgctgg cggccgggct
     6121 gatgcagacg ctggaggaca agctctactc cgagatcatc atgacgcggg tcaccgtgcc
     6181 ggtgggcgag gacatcggct tcctacccgg caccgaggaa gagaagatga cgccctggat
     6241 gggcgcgctc gaggacaacc tcgacgtgct caacaagacc gacgaggacg ccggcgaatg
     6301 gggccgcgcc gccacccgcg atctgatccg ctcgcgcatc aaggtgaagt cgctgaactt
     6361 catgcgcggc cgcaccttca tcaacaagtt cctgatcctg gacgaggcgc agaacctcac
     6421 gccgaagcag atgaagacta tgatcacgcg cgccgggccg ggcacgaaag tggtgtgcct
     6481 gggcaacatc gcgcagatcg acacgcccta cctcaccgaa ggcagctccg gcctgaccta
     6541 cgtggtggat cgcttcaagg gctggagcca cagcggccac gtaaccctgc agcgcggcga
     6601 gcgctcgcgg ctggcagatc acgcggcgga gatcttgtag cgagttcgca tacccgggag
     6661 accctcatgc ccttcgccga ttcagcaggc gtacgcctgc actacgagga aaacggcagc
     6721 ggaacgccag tcatcttcgt gcacgagttc gcggccgatt gccgcagctg ggagccgcag
     6781 gtgcgctatt tcgcgcggcg ctaccggtgc atcactttca acgcacgcgg ctacccgccg
     6841 tccgatgtgc cgcgcgatcc ggaagcgtac agccagcggc acgctgtcga cgacatcgcc
     6901 gccgtactgc accacctgaa gatcgacagg gcccacatct gcggactgtc gcaaggcgga
     6961 tacgccaccc tgcacttcgg catccactac ccgcggatgg cgctttcgct tgcgatatgc
     7021 ggcgcgggcc acggttcgga tcccggcacg cgcgctcagt ttttgaagga tggggccgag
     7081 ctcgccgagc gcatcgagcg cctgggcatg gaggaaggca tcaagcacta tgcggccagc
     7141 cccacccgcc tgccgctgcg gcacaaggat ccgcgcggct acgaagagtt ttaccgccag
     7201 ttcgccgagc actcgaacat cggctcggcg ctcaccatgc gcggctatca gctgcggcgg
     7261 ccgaccatct acgagctgga gcgcgacatc gagaagatcg acgtgcccac cttgatcgtc
     7321 accggcgacg aagacgggcc gtgcctggag ccggggatat tcatgaagcg caagatccgc
     7381 tcggcggggc tgctcgtcat tcccaacagc gggcacagca tcaacctgga agaggtcgat
     7441 gccttcaacc gtgcgctgct cg
//
LOCUS       DASPGE010000151         3209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_512260, whole genome shotgun sequence.
ACCESSION   DASPGE010000151 DASPGE010000000
VERSION     DASPGE010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512260"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="VD791_04815"
     CDS             <1..1668
                     /locus_tag="VD791_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV07326.1"
                     /translation="LGGPLGFWGGIVLAALAVGLVGVAMEMLLLRRIYHAPELFQLLA
                     TFGVVLIVQDAALWIWGREDLFAGRAPGLRGTVEILGERFPQYHLFLIFLGPVVLGLL
                     WLLFHRTRWGVLVRAATQDREMVGALGVNQRWLFTSVVFLGSALAGLGGALQIPKEAV
                     NLQMDVHVIAEAFVVVVIGGMGSVAGAFLASLLVGLLSAFGILIFPQLTLVLTFLVMA
                     VVLVLRPYGLLGKPEARPRTAPQAAERPLAPAPRPVLLAGGVVLAALVALPAVAGEYA
                     ELVATEIFILALFAASLHFILGVGGLVSFGHAAYFGIGAYACGLLLQRLALPMELGVL
                     AAPLAAGALAVLFGWFCIRLSGVYLAMLTLAFAQILWSIAFQSAWTGGDNGILGVWPA
                     AWLGSKTAYYYFALALAVLSLLFLRRAIFAPFGYALRAGRDSPLRAEAIGIDVRRQQW
                     AAFALAGAFAGLAGALHAFHKGSVFPNVLSIPQSVDALVMVLLGGVQTLGGPIVGAAV
                     YHTLLAEVMRSVEYWRLIVGAVIVLLVVAFPQGIVGFAQRLVRRDAH"
     gene            1672..2442
                     /locus_tag="VD791_04820"
     CDS             1672..2442
                     /locus_tag="VD791_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV07327.1"
                     /translation="MLLLTVTNLSKSFGGVQAVREVSFSLQPGELLALIGPNGAGKST
                     CFNMLNGQLRPDSGTVLLEGQDITGHPPRAVWRRGVGRTFQVAATFGSMTVRENVQMV
                     LLSRERRLLRLWRRAGDQYRDQAMALLARVGMASQAERACALLAYGDVKRVELAMALA
                     NDPRLLLMDEPTAGMAPRERNDLMALIAELTRERGLGVLFTEHSMDVVFAYAQRMLVL
                     SRGRIIAEGDPAAVRENAEVRAVYLGTGTAFQAHAALS"
     gene            2444..3160
                     /locus_tag="VD791_04825"
     CDS             2444..3160
                     /locus_tag="VD791_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV07328.1"
                     /translation="MPAESVLAVRQLSAWYGRAQILFDVGLDVARGEVVALMGRNGAG
                     KSTTLKSIMGLVERTRGEVVFRGKSLRGAEPFDIARLGLGYVPEDRRIFTELTVRENL
                     EVGRQPPRPGAPGWTEEKLFRVFPNLAEMQARPGGQMSGGEQQMLTVARTLMGNPLLV
                     LLDEPSEGIAPRIVEEMANMILELKREGLSVLLCEQNLNFAALVSDRAYVIEKGEIRY
                     SGTMRELAANDAVKREHLSL"
BASE COUNT          401 a         1055 c         1185 g          568 t
ORIGIN      
        1 ctcggtgggc cgctcggttt ctggggcggc atcgtgctgg ccgcgctcgc cgtcggactg
       61 gtgggcgtgg cgatggagat gctgttgctg cgccggatct accacgcacc cgagctgttc
      121 cagttgctcg ccaccttcgg cgtggtgctg atcgtgcagg acgcggcgct gtggatctgg
      181 ggacgcgaag atctgttcgc ggggcgcgcg ccgggcctca ggggcaccgt cgagatcctg
      241 ggcgagcgct ttccgcagta tcacctgttc ctcatcttcc tcggcccggt cgtgctggga
      301 ctgctctggc tgctgttcca tcgcacgcgc tggggcgtgc tggtgcgcgc ggccacgcag
      361 gaccgcgaga tggtcggggc gctgggggtg aatcagcgct ggctgttcac cagcgtggtg
      421 tttctcggct cggcgctcgc cggcctgggc ggggcgctgc agatcccgaa ggaggcggtg
      481 aacctgcaga tggacgtgca cgtcatcgcg gaagccttcg tggtggtggt gatcggcggc
      541 atgggcagcg tggccggcgc tttcctggcc tcgctgctgg tcggtctgct gtcggcgttc
      601 ggcatcctga tcttcccgca gctcaccctg gtgctcactt tcctggtgat ggcggtggtg
      661 ctggtgttgc gcccctatgg cctgctgggc aagccggagg cgcgcccccg caccgcgccg
      721 caggccgcgg agcggccgct ggcgccggcg ccacggccgg tcctgctcgc cggcggcgtg
      781 gtgctcgcgg ccctggtcgc gctgccggcg gtggccggcg aatatgccga gctggtcgcc
      841 acggagatct tcatcctggc gctgttcgcc gccagcctgc acttcatcct gggcgtcggc
      901 ggcctggtct ccttcggtca tgccgcctac ttcggcatcg gcgcgtacgc ctgcggcctg
      961 ctgctgcagc ggctcgccct gccgatggag ctcggcgtgc tggccgcgcc gcttgccgcc
     1021 ggggcgctgg cggtgttgtt cggctggttc tgcatccggc tctcgggcgt gtacctggcc
     1081 atgctcacgc tcgccttcgc ccagatcctg tggtcgatcg ccttccagtc ggcctggacc
     1141 gggggcgaca acggcattct cggcgtgtgg cccgcggcgt ggctcggctc caagaccgcc
     1201 tactactact tcgcgctggc gctggcggtg ctgagcctgc tgttcctccg gcgcgcgatc
     1261 ttcgcgccgt tcggctacgc gctgcgcgcg gggcgcgact cgccgctgcg cgcggaggcc
     1321 atcggcatcg acgtgcgccg ccagcaatgg gcggcgttcg cgctggccgg cgctttcgcc
     1381 ggcctggcag gcgcgctgca cgcgttccac aagggcagcg tgtttcccaa cgtgctgtcg
     1441 atcccgcagt cggtcgacgc gctggtcatg gtgctgctcg gtggcgtgca gacgctgggc
     1501 ggcccgatcg tcggcgccgc cgtgtaccac acgctgctcg ccgaggtgat gcgcagcgtc
     1561 gagtactggc gtctgatcgt cggcgcggtc atcgtgctgc tggtagtggc gtttccacag
     1621 ggaatcgtcg ggttcgcgca gcgcctggtg cgccgagatg ctcattgacc tatgctcctg
     1681 ctcacggtta ccaacttgtc caagtccttc ggcggcgtgc aggccgtgcg cgaggtgtcc
     1741 ttcagcctgc agcccggcga gctgctcgcg ctgatcgggc cgaacggcgc cggcaagtcc
     1801 acttgcttca acatgctcaa tggccagctg cgccccgaca gcggaaccgt cctgctggag
     1861 ggccaggaca tcaccggtca tccgccgcgc gcggtctggc ggcgcggggt gggccgtacc
     1921 ttccaggtgg cggcgacctt cggctcgatg acggtgcgcg agaacgtgca gatggtgttg
     1981 ctgtcgcgcg agcgacgtct gctgcggctg tggcgccgcg ccggcgatca gtatcgggac
     2041 caggcgatgg ctttgctcgc gcgcgtgggc atggcgagcc aggccgagcg tgcgtgcgcg
     2101 ctgctcgctt acggcgacgt caagcgcgtc gagctcgcca tggcgctcgc caacgacccg
     2161 aggctgctgc tgatggacga gcccacggcg ggtatggcgc ccagggagcg caacgacctg
     2221 atggcgctga tcgcggagct aacgcgcgaa cgcggcctcg gggtgctgtt caccgagcac
     2281 agcatggacg tggtgttcgc ttacgcgcaa cgcatgctgg tgctctcgcg cgggcgcatc
     2341 atcgccgagg gcgatcccgc cgcggtgcgc gagaacgccg aggtgcgggc ggtgtatctg
     2401 ggcaccggca cggcgttcca ggcgcacgcg gcgctgtcct gacatgccgg ccgaaagcgt
     2461 gctcgcagtg cggcagctca gcgcctggta cggccgggcg cagatcctgt tcgacgtcgg
     2521 cctggacgtc gcgcgcggcg aggtggtcgc gctgatgggc cgcaacggcg cgggcaagtc
     2581 caccacgctg aagtcgatca tggggctggt cgagcgcact cggggggaag tggtgttccg
     2641 cggaaagtcc ctgcgcggag cggaaccttt cgatatcgcc cgcctcggcc tgggatacgt
     2701 gcctgaggac cgtcgcatct tcaccgagct caccgtgcgg gagaacctgg aagttggacg
     2761 tcagcccccg cgaccgggcg cgccgggctg gaccgaggag aagctgttcc gcgtgttccc
     2821 caacctcgcc gagatgcagg cgcgcccggg cggccagatg agcggcggcg aacagcagat
     2881 gctcacggtg gcgcgcacgc tcatgggcaa ccccctcctg gtgctgctcg acgagccctc
     2941 ggaaggcatc gccccgagaa tcgtcgaaga gatggcgaac atgatcctgg agctgaagcg
     3001 ggaggggctc agcgtcctgc tgtgcgagca gaacttgaat ttcgcggccc tggtgagcga
     3061 ccgcgcttac gtcatcgaga agggcgagat ccgctacagc gggacgatgc gcgagctggc
     3121 ggcgaacgac gcggtgaagc gcgagcacct gagcctgtag actagcggga ggaggaaacc
     3181 atgccgaccg agatccccga agtgccctg
//
LOCUS       DASPGE010000152         2632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_395113, whole genome shotgun sequence.
ACCESSION   DASPGE010000152 DASPGE010000000
VERSION     DASPGE010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395113"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1869)
                     /locus_tag="VD791_04830"
     CDS             complement(<1..1869)
                     /locus_tag="VD791_04830"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HXV07329.1"
                     /translation="MWQPTEASIEQAQLTQFARHAVRKHRLDLNTFAHFYRWTVENPE
                     AFWTEVWEWCGVRASRKGGTVLVDGDKMPGARWFPEARLNLAENLLRRGDRGDALVFW
                     DERGPQRRVSYSDLTSDVSRAAQALAALGLRAGDRAAAFIPNLPETMMFALAALSQGI
                     VWSSCSPDFGVDGVLERFGQIEPKVLFVADGYLYNGKRHDVLERVREIAERLPSLRKV
                     VVVPYLDARPDIADIPKAVLLEEWLRRHTPGDIAWAQLPFDHPAYILFTSGTTGKPKC
                     IVHGAGGTLLAALKMYKLHFDVRPGDHFFYYTTCNWVMWNLLFHALAAEASVMLYDGS
                     PFAREADILFEYAQRERFSHFGTSAKFIDAVAKRGLKPRERYPLPGLRMIVSTGSPLV
                     AESYDYVYREVKNDVCLSSISGGTDIMAAFADANAILPVYRGELQCRSLGLAVEVFDE
                     QGNAVVGKKGELVCTRPFPSMPLGFWNDRGGEKYHAAYFAKYPNVWCHGDWCELTERG
                     TMIVYGRSDATLNPGGVRIGTAEIYGQVEKIPEVEESVAIGQLWPPDKPTDTRVVLFV
                     KLREGLRLDAALEERIRRQIGDHTTKRHVPAKIVQVPDIPRTKNNKVVELAVKAVVH"
     gene            1916..>2632
                     /locus_tag="VD791_04835"
     CDS             1916..>2632
                     /locus_tag="VD791_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07330.1"
                     /translation="MSSPRVLFVLFTLSGFTGLVYESVWSHYLKLFLGSAAFAQSFVL
                     AAFMGGMALGAWLASRAGAQRNLLAAYGWIEALIGVAALAFHFVFTGLTDWSLETVIP
                     AIGDPAAIELYKYALCALLIVPQTVLLGMTFPLMSGAVIRRFPTDAAGGPAGGHHLAM
                     LYFTNSIGAAAGALVSAFLLMGWLGMPGTMRLAGVLNLVLAVLVLSLARGTEPRPGVA
                     GSAPGALPARTGLVVLFLAAA"
BASE COUNT          367 a          920 c          894 g          451 t
ORIGIN      
        1 gtgcaccacg gccttcaccg ccagctccac caccttgttg ttcttggtgc gcgggatgtc
       61 gggcacctgc acgatcttcg ccggcacgtg ccgcttggtg gtgtggtctc cgatctgacg
      121 gcgaatcctc tcctcgagcg ccgcatccag gcgcaggccc tcgcgcagct tgacgaacag
      181 caccacgcgc gtgtccgtgg gcttgtctgg cggccagagc tgcccgatgg cgaccgattc
      241 ctcgacctcg gggatcttct ccacctggcc gtagatctcc gcggtgccga tcctcacgcc
      301 cccggggttg agcgtggcgt ccgagcgtcc gtagacgatc atcgtgccgc gctcggtgag
      361 ctcgcaccag tcgccgtggc accacacgtt cgggtacttg gcgaaatagg ccgcgtggta
      421 cttctcgccg ccgcggtcgt tccagaagcc gagcggcatg gaagggaagg gcctggtgca
      481 caccagctcg cccttcttgc ccaccacggc gttgccttgc tcgtcgaata cttccacggc
      541 gaggccgagc gagcggcact gcagctcgcc gcgatacacc ggcaggatgg cgttggcgtc
      601 ggcgaacgcg gccatgatgt cggtgccccc ggagatggac gacaggcaca cgtcgttctt
      661 cacctcccgg tagacgtagt cgtagctctc ggcgacgagc ggcgagccgg tcgacacgat
      721 catgcgcagg cccggcagcg gatagcgctc gcgcggcttc aggccgcgct tggcgacggc
      781 atcgatgaac ttggccgagg tgccgaaatg gctgaagcgc tcgcgctggg cgtactcgaa
      841 caggatgtcg gcctcgcgcg cgaagggcga gccgtcgtac agcatcacgc tcgcctccgc
      901 ggcgagcgcg tgaaacagca ggttccacat cacccagttg caggtggtgt agtagaagaa
      961 gtggtcgccg ggccgcacgt cgaagtgcag cttgtacatc ttgagcgccg cgagcagggt
     1021 gccgcccgcg ccgtgcacga tgcacttggg cttgccggtg gtgcccgagg tgaacaggat
     1081 gtacgccggg tggtcgaagg gcagctgcgc ccaggcgatg tcgccgggcg tgtgccggcg
     1141 cagccactcc tccagcagca ccgccttggg gatgtcggcg atgtccgggc gggcgtccag
     1201 gtacggcacg accaccacct tgcgcaggct cggcagccgc tcggcgattt cgcgcacgcg
     1261 ctccagcacg tcgtggcgct tgccgttgta gagatacccg tcggcgacga acagcacctt
     1321 gggctcgatc tggccgaagc gctcgagcac gccgtcgacg ccgaaatccg gcgagcacga
     1381 ggaccacacg atgccctggg aaagcgccgc cagcgcgaac atcatggttt ccggcaggtt
     1441 gggaatgaag gccgccgcgc ggtcgccggc gcgcaggccg agcgccgcca gcgcctgcgc
     1501 ggcgcgcgac acgtcgctcg tcaggtcgga gtagctgacg cgtcgctgcg gcccgcgctc
     1561 gtcccagaac accagcgcgt cgccgcggtc tccccggcgc agcaggttct ccgcgagatt
     1621 gagccgcgcc tccggaaacc agcgcgcgcc gggcatcttg tcgccgtcga ccaggacggt
     1681 gccgcccttg cgcgaagcgc gcacgccgca ccattcccag acctcggtcc agaacgcttc
     1741 cgggttttcg accgtccagc ggtaaaaatg cgcaaaagta ttgagatcga gccggtgttt
     1801 tcgcaccgcg tgacgcgcga actgcgtaag ctgcgcctgc tcgatcgacg cttcggtcgg
     1861 ttgccacatc ggctgcatgc ccgggattat cccgccgata cgataggatt gctgcatgag
     1921 ctcgccgcgg gttcttttcg tcctgttcac gctgtccggc ttcaccgggc tggtgtacga
     1981 atccgtctgg tcgcactacc tgaagctgtt cctcggctcg gcggcgttcg cccagtcctt
     2041 cgtgctcgcc gccttcatgg gcggcatggc gctcggcgcc tggctggcga gccgcgccgg
     2101 cgcgcagcgc aacctgctcg ccgcctacgg ctggatcgag gcgctgatcg gcgtcgcggc
     2161 gctcgccttc cacttcgtgt tcaccgggct caccgactgg tcgctggaga ccgtgattcc
     2221 ggcgatcggc gacccggcgg cgatcgagct ctacaagtac gcgctgtgcg ccctgctgat
     2281 cgtgccgcag accgtgctgc tcggcatgac cttcccgctg atgagcgggg ccgtgatccg
     2341 gcgctttccg accgacgccg ccggcggccc cgccggcggg caccacctgg cgatgctgta
     2401 tttcacgaac tccatcggcg cggccgcggg cgcgctggtg tccgccttcc tgttgatggg
     2461 ctggctgggc atgccgggca cgatgcgcct cgccggggtg ctgaacctcg tgctcgcggt
     2521 cctcgtgctc tcgctcgcgc gcggcaccga gccgcgcccg ggcgtcgccg gcagcgcgcc
     2581 cggcgcgctg ccggcgcgca ccgggctggt cgtgctcttt ctcgccgccg cg
//
LOCUS       DASPGE010000153         2868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1214930, whole genome shotgun sequence.
ACCESSION   DASPGE010000153 DASPGE010000000
VERSION     DASPGE010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214930"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VD791_04840"
     CDS             <1..343
                     /locus_tag="VD791_04840"
                     /inference="COORDINATES: protein motif:HMM:NF014543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07331.1"
                     /translation="YRDGFGVYTWPNGDRFEGPWVKDQRQGYAAMEARRLQARAAQEE
                     AFKPGVSVCSLGAAAMAQLAFVKGSVDSFDGAKLSVKLLELPAALTQGGEVLHVGQVV
                     VEDPAQWAPCF"
     gene            362..2473
                     /locus_tag="VD791_04845"
     CDS             362..2473
                     /locus_tag="VD791_04845"
                     /inference="COORDINATES: protein motif:HMM:NF017857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HXV07332.1"
                     /translation="MQLCRRFVVLALAASGLGLDATAAWSQVGLNGQTGLVYMPDARI
                     APDGTLRFGVSYADPYLSGWSSIALFPFLEVSGKYTRIEGVESGFEDYGDYKDKEADF
                     KLRLLPEGKYWPQVAFGMQDFFGTEIFGARYLALSKQLGDFDFTLGYGDDRIDGVFGG
                     LRYTPRAFPGLSFVAEYDAYDYSEDTGAAVTGVIDRNKGPVGGLEYRWKWLAAQLSYG
                     HDEVGVNAYVSVPLNQKEFVPKLKEPPPYTKITPRPTIAQWNADPAHRARMARALHEQ
                     YFKRVRLGLQGATLRVELTNSRINQMSRAVGRAARTILLTSPLETRELQITYTVNDLP
                     FATYTFADLDKLQRYFNGMIPRQQLEPSISMRYADPRAGDVKLETAAMLDAFDQQYRM
                     MVLDDTEGDFLSLRGEGAGIGGFKLSPKVSFYVNDPSGAFRYDIYLLASYKRELLKRL
                     FFEASARVTIVEDVSEVTTESNSLLPHVRSDIAEYAREQGWIGLERLLLNRYFAPAPR
                     FYARASVGYYEQMFGGGGGQVLYLSQSGKWAADIAADWVKQRDFDGGFGFQDYSTVTS
                     LASVHFKVPLLPATTATVRAGRFLAEDEGVRFELKRRFGSGIEVGFWYTRTNGDDITS
                     PGSPDDPYYDKGVFLSVPLSPLLLKDSQARADFSLAPWTRDVGQMVTSPGDLYDMFER
                     PVRDFKEEGGLTRFGDFDDKN"
     gene            2501..>2868
                     /locus_tag="VD791_04850"
     CDS             2501..>2868
                     /locus_tag="VD791_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07333.1"
                     /translation="MRAMRSAAVLGVLVCFTNWTMAATVQVVRLQGSVAAQVGAGPEQ
                     MLRAGDTLQQGTTISTGQNSNVVLRFDDGQVVALKSLSRFTIESYRYDAKDPGAGQMI
                     LSLLSGGMRAITGLVANSNRS"
BASE COUNT          483 a          929 c          984 g          472 t
ORIGIN      
        1 ctatcgggat gggttcggcg tgtacacctg gccgaacggg gatcgattcg aaggcccctg
       61 ggtgaaagac cagcgccagg gttacgcggc catggaggcg cgccggctgc aggcgcgggc
      121 cgctcaggag gaggcgttca agccgggcgt ttccgtgtgc tcgctgggag cggccgcaat
      181 ggcgcagctc gcctttgtga aggggtcagt ggacagcttc gatggcgcca agttgagcgt
      241 caagttgctc gagctgcccg ccgcgttgac gcagggcggc gaggtgctcc acgtcggaca
      301 ggtcgtcgtg gaggacccgg cgcagtgggc gccgtgcttt taggcccgcg gcggagcgca
      361 ggtgcagctg tgcaggcgct tcgttgtact tgcgctagcc gcaagcggtc tgggcctgga
      421 cgccaccgct gcctggtccc aggtggggct caacggtcag accgggctgg tgtacatgcc
      481 cgatgcccgc atcgcgcccg acggcacgct gcgcttcggt gtcagctacg ccgatcccta
      541 tctgtccggc tggtccagca tcgcgctgtt tcccttcctc gaggtgtccg gcaagtacac
      601 ccgcatcgag ggcgttgaaa gcggcttcga agactacggc gactacaagg acaaagaagc
      661 cgacttcaag ctgcgcctgc tgcccgaagg caagtactgg ccgcaggtcg ccttcggcat
      721 gcaggacttc ttcggcaccg agatcttcgg cgcgcgctac ctggcgctga gcaagcagct
      781 cggcgatttc gacttcacgc tggggtacgg cgacgaccgc atcgacgggg tattcggcgg
      841 cctgcgctac accccgcgcg cgtttcccgg cctgtcgttc gtcgccgagt acgacgcgta
      901 cgattactcg gaggacaccg gtgcggccgt caccggcgtg atcgaccgca acaaggggcc
      961 ggtgggcggc ctggagtatc gctggaagtg gctcgccgcg cagttgagct acggccacga
     1021 cgaggtcggc gtgaacgcct acgtgtcggt tccgctgaac cagaaggagt tcgtgcccaa
     1081 gctcaaggag ccgccgccct acaccaagat cacgccgcgg cccaccatcg cgcagtggaa
     1141 cgccgacccg gcgcatcgcg cccgcatggc gcgtgcgctg cacgagcagt acttcaaacg
     1201 cgtgcgcctg ggcctgcagg gcgccacgtt gcgcgtggag ctcaccaaca gccgcatcaa
     1261 ccagatgagc cgtgccgtgg ggcgcgcggc gcgcaccatc ctgctcacca gtccgctcga
     1321 gacgcgcgag ctgcagatca cctacacggt gaacgatctg ccgttcgcca cctacacctt
     1381 cgccgacctg gacaagctgc agcgctattt caacggcatg atcccgcgcc agcagctcga
     1441 gccgagcatc tcgatgcgct acgccgatcc gcgcgccggc gacgtcaagc tggaaaccgc
     1501 ggccatgctg gatgcattcg accagcagta ccgcatgatg gtgctggacg ataccgaggg
     1561 cgacttcctc agcctgcgcg gcgagggcgc gggcatcggc gggttcaagt tgagccccaa
     1621 ggtctcgttc tacgtcaacg atcccagcgg cgcgttccgc tacgacatct acctgctcgc
     1681 cagctacaag cgggagctgc tcaagcgcct gttcttcgaa gcgagcgcgc gcgtcaccat
     1741 cgtcgaggac gtgagcgagg tcaccaccga atcgaacagc ctgctgcctc acgtgcgctc
     1801 ggatatcgcc gagtacgcgc gcgagcaggg ctggatcggg ctcgagcgtc tgctgctcaa
     1861 ccggtacttc gcacccgcgc cgcgcttcta tgcgcgcgcc tcggtcggct actacgagca
     1921 gatgttcggc ggcggcggcg gacaggtgct gtacctgtcg cagtctggca agtgggcggc
     1981 cgacatcgcg gccgactggg tgaagcagcg cgacttcgac ggcggcttcg gtttccagga
     2041 ctattcgacg gtcaccagcc tcgccagcgt acacttcaag gtgccgcttc tgccggcgac
     2101 cacggcgacg gtgcgcgccg gccgctttct cgccgaggac gagggcgtgc gcttcgagct
     2161 gaagcgccgc ttcgggtcgg gcatagaggt gggtttctgg tacacccgca ccaacggcga
     2221 cgacatcacc tcgccggggt cgccggacga cccgtattac gacaaaggag tattcttgag
     2281 cgtgccgctg agcccgctgc tgctcaagga cagccaggcg cgtgccgatt tctcgctggc
     2341 gccctggacg cgggacgtgg ggcagatggt gacgtcgccc ggcgacttgt acgacatgtt
     2401 cgagcgtccc gtgcgcgact tcaaggagga aggggggctc acgcgcttcg gcgacttcga
     2461 tgacaagaac tgacggcaga cgaacaacat ggggatgaag atgcgagcga tgcggtcggc
     2521 ggcggtgctg ggggtgctgg tgtgcttcac gaactggacg atggcggcga ccgtgcaggt
     2581 ggtgcgcctg caaggcagcg tcgcagcgca ggtgggcgcc ggcccggagc agatgttgcg
     2641 ggccggcgac accctgcagc aaggcaccac catcagcacg ggccagaaca gcaacgtggt
     2701 attgcgcttc gacgacgggc aggtggtggc gctgaagtcc ctgtcgcggt tcaccatcga
     2761 atcctatcgc tacgacgcca aggatccggg cgcggggcag atgatcctgt cactgctgtc
     2821 cggcggcatg cgcgccatca ccggcctggt ggccaacagc aaccgctc
//
LOCUS       DASPGE010000154         4131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_746376, whole genome shotgun sequence.
ACCESSION   DASPGE010000154 DASPGE010000000
VERSION     DASPGE010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746376"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1612)
                     /gene="thiC"
                     /locus_tag="VD791_04855"
     CDS             complement(<1..1612)
                     /gene="thiC"
                     /locus_tag="VD791_04855"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HXV07334.1"
                     /translation="MNANPKFIAATARVDAAAVQPLPGSRKVYLQGSRPDIRVPLREI
                     SQADTPAAFGAEANPRITVYDTSGPYTDPDASVDIRTGLPALRAAWIEARGDTEELPG
                     PSSRYGRERLLDSRLAELRFHLERKPRRARAGKTVTQMHYARAGVVTPEMEFIALREN
                     QRCEGLSEMLRRQHAGQRFGASIPAVITPEFVRDEVARGRAIIPANVNHPESEPMIIG
                     RNFLVKINANIGNSAVTSGIAEEVAKMTWAIRWGGDTVMDLSTGKHIHETREWIVRNS
                     PVPIGTVPIYQALEKVDGKAEELTWEIFRDTLIEQAEQGVDYFTIHAGVRLPYIPLTA
                     KRVTGIVSRGGSIHAKWCLAHHEENFAYTHFEEICEIMKAYDVSFSLGDGLRPGSIAD
                     ANDEAQFGELETLGELTQIAWKHDVQVMIEGPGHVPMHLIHENMTKQLEVCHEAPFYT
                     LGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPDKKDVKDGVIAYKI
                     AAHAADLAKGHPGAQMRDNALSRARFEFR"
     gene            1671..2324
                     /locus_tag="VD791_04860"
     CDS             1671..2324
                     /locus_tag="VD791_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HXV07335.1"
                     /translation="MDIERARTNMIESQIRTWEVLDQRVLDTLSAVKREEFVPERYRP
                     LAFVDMEIPLAHGEVMLAPKLEARMLQELTLKESDRVLEVGTGSGYMTALLASLAGHV
                     YSVELHPDLSATAAGKLAAHGIVNATLEVGDAAQGWSRHAPYDAILLTGSVPVLPPRL
                     RAQLRPGGRLLAVVGDPPVMTARLITCVTDGAWNDTGLFETCIAPLRNVPQPERFVF"
     gene            2434..3768
                     /locus_tag="VD791_04865"
     CDS             2434..3768
                     /locus_tag="VD791_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HXV07336.1"
                     /translation="MRLRLIACAAGLLAGGPLHAEDLMDIYRDALIQDPVFAAQRAAY
                     QATKERKVQARALLLPNINATANANYNDIDTQFDGTTAATSQLEGQRDYESYGGAVNI
                     TQPLFRRQNNVTVQQAGIQVEQAGNQLAQAGQELMLRVAQGYFDVLLAQFELLTVEAQ
                     KAATAEQLAQAKRNFEVGTATITDTYDAQARYDLIVARELASRNDIQVRINALQQIIG
                     RPPGPLAKLDGPVTLNPPEPNDMEQWVQAAYANSQQVQVAKANADLATKEVDKNRFGH
                     YPTLDAVGNLSYNSQGDSNFGVGQDVTSAVVGLQLAVPLYSGGSISSRVREAISNRTR
                     AEQDLENTRRTVAQATRQAFLAVNTGLSEINALQQAVASNKLSVEASKLGQEVGVRTQ
                     VDVLNAQGLLFQAQRDLARAYYVSILSQLRLKASVGRLSEVDLENVNRLLAK"
     gene            3771..>4131
                     /locus_tag="VD791_04870"
     CDS             3771..>4131
                     /locus_tag="VD791_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HXV07337.1"
                     /translation="MNDLRAEKAALRQAVLARRNALPHTARADLSRRITAALLAQPAL
                     ATAGTVAAYLSFGSEFDTADFVAHVLGAGKRLVLPRVNRERRRLDLHAVQDLDAGLVP
                     GVWGIREPSPERCPLATL"
BASE COUNT          677 a         1419 c         1379 g          656 t
ORIGIN      
        1 agcggaactc gaaacgcgcc cggctcaacg cattgtcgcg catctgcgcg ccgggatggc
       61 ctttggccag gtcggctgca tgcgccgcaa ttttgtaggc gatgacgccg tctttcacgt
      121 cctttttgtc gggcaagccg agatgctcct tgggcgtcac gtaacaaagc atcgcggtgc
      181 cgtaccagcc gatcatcgcc gcgccgatcg cgctggtgat gtggtcgtag cccggcgcga
      241 tatcggtggt cagcggcccg agggtgtaaa acggcgcctc atggcacact tccaactgct
      301 tggtcatgtt ttcatgaatc aagtgcatcg gcacgtgccc cggcccctcg atcatcacct
      361 gaacgtcgtg tttccaggcg atttgggtga gctcccccag cgtttcgagc tcgccaaact
      421 gcgcctcgtc attggcgtca gcgattgagc ccggccgcag tccgtcgccc aatgaaaacg
      481 agacgtcgta ggccttcatg atctcgcaga tctcttcgaa atgggtgtac gcaaaattct
      541 cttcatgatg ggccaagcac cacttggcat ggatcgagcc gccgcgcgac acgatgccgg
      601 tgacgcgctt cgccgtcagc gggatgtagg gcaggcgcac gccggcgtgg atggtgaagt
      661 agtccacccc ttgctcggcc tgctcgatca gggtgtcgcg gaagatctcc caggtcagct
      721 cctcggcctt gccgtccacc ttctccagcg cctggtagat cggcacagtg ccgatcggca
      781 ccgggctgtt gcgcacgatc cactcgcgcg tctcgtggat gtgcttgccg gtggacaggt
      841 ccatcaccgt gtccccgccc cagcggatcg cccaggtcat cttcgccact tcctcggcga
      901 tgccggaagt gaccgcagag ttgccgatgt tggcgttgat cttcaccagg aagttgcggc
      961 cgatgatcat cggctcgctc tccgggtgat tcacgttcgc cgggatgatg gcgcgcccgc
     1021 gcgccacctc gtcgcgcacg aactcgggcg tgatgaccgc cgggatcgaa gcgccgaaac
     1081 gctgccccgc gtgctgtcgc cggagcatct cggagagccc ctcgcagcgc tggttctcgc
     1141 gcaaggcgat gaactccatc tccggcgtta ccacgccggc gcgcgcgtag tgcatctggg
     1201 tcaccgtctt gcctgcgcgt gcgcgccgcg gcttgcgctc gaggtggaag cgcagctcgg
     1261 cgagcctgga gtcgagcagg cgctcgcggc catatcggga ggacggcccg ggcagctcct
     1321 cggtgtcgcc gcgcgcctcg atccaggccg cgcgcagcgc cggcagaccc gtgcggatgt
     1381 cgacggacgc gtcggggtcg gtgtaaggcc ccgaggtgtc gtacacggtg atgcgcgggt
     1441 tggcctcggc gccgaaggcc gccggcgtgt ccgcctggct gatctcgcgc aacggcacgc
     1501 ggatatcggg ccgcgagcct tgcaggtaca ccttgcgcga gccggggagg ggctgcacgg
     1561 ctgcggcgtc cacgcgtgcg gtggcggcga tgaacttggg attggcgttc atttgcagga
     1621 gtcggtcgta gattttgtgg gataattcta cgccctttgc cgcccggcac atggacatcg
     1681 agcgcgcccg caccaacatg atcgaaagcc agatccggac gtgggaagtc ctggatcagc
     1741 gcgtgctcga cacgctgtcg gcggtcaagc gcgaggagtt cgtccccgaa cgctatcggc
     1801 cgctggcctt cgtcgacatg gagatcccgc tggcccatgg cgaggtgatg ctggcgccca
     1861 agctcgaggc gcgcatgctg caggagctga cgctgaagga gagcgaccgc gtgctggagg
     1921 tgggcaccgg cagcgggtac atgaccgcgc tgctggcgag cctggccggc cacgtctaca
     1981 gcgtcgagct gcacccggat ctctcggcga ccgcggccgg caagctcgcc gcacacggca
     2041 tcgtcaatgc gacgttggag gtcggcgatg ccgcccaggg ctggtcccga cacgcgccgt
     2101 acgacgccat cctgctcacc ggctcggtgc cggtgctgcc tccccggttg cgggcacagc
     2161 tcaggccggg gggccggctg ctggcggtgg tcggcgatcc gccggtgatg accgcgcgcc
     2221 tgatcacctg tgtgaccgac ggcgcctgga acgacaccgg gctgttcgaa acctgcatcg
     2281 cgccgctgcg caacgtcccg cagccggagc ggtttgtgtt ctgacccggc aaagctggcc
     2341 cgggccttgc tcgcggcgag cgggctgggc ggcgcaagcc gccccgagcg ccggccgacg
     2401 aaacgggaac caccctcgaa gggaccgaag aatatgcgcc tgagactgat cgcctgcgct
     2461 gccggactgc tcgccggcgg cccgctgcac gcggaagacc tgatggacat ctaccgggat
     2521 gccctgatac aggacccggt attcgcggcg caacgggccg cttatcaggc caccaaggag
     2581 cgcaaggtgc aggcccgcgc cctgctgctg ccgaacatca acgccaccgc caacgccaac
     2641 tacaacgaca tcgacaccca gttcgacggc accacggccg ccacctccca gctggaaggc
     2701 cagcgcgatt acgagtcgta cgggggggcg gtgaacatca ctcagcccct gttccgccgc
     2761 cagaacaacg tcaccgtgca gcaggccggc atccaggtcg agcaggccgg caaccagctc
     2821 gcccaggccg gccaggagct gatgctgcgc gtggcgcagg gctacttcga cgtgctgctc
     2881 gcccagttcg agctgctcac cgtcgaggcg cagaaggccg ccaccgccga gcagctggcg
     2941 caggccaagc gcaacttcga ggttggcacc gccaccatca ccgacaccta cgatgcccag
     3001 gcacgctacg acctgatcgt cgcgcgcgaa ctggccagcc gcaacgacat ccaggtgcgc
     3061 atcaacgccc tgcagcagat catcggccgt ccccccgggc ccctcgccaa gctggacggg
     3121 ccggtcacgc tgaacccgcc cgagcccaac gacatggagc agtgggtgca ggccgcctac
     3181 gccaacagcc aacaggtcca agtggccaag gccaacgccg acctcgcgac caaggaggtg
     3241 gacaagaacc gcttcggcca ctatccgacc ctggacgccg tgggcaacct cagctacaac
     3301 agccagggcg actccaattt cggcgtgggc caggacgtga ccagcgccgt ggtgggcctg
     3361 cagctcgccg tgccgctgta cagcggcggg tcgatcagct cgcgggtgcg cgaagcgatc
     3421 tccaaccgca cgcgcgccga gcaggacctg gagaacaccc gccgcaccgt ggcccaggcc
     3481 acgcgccagg cgtttctggc ggtcaacacc ggcctgtccg agatcaacgc cctgcagcag
     3541 gcggtggcga gcaacaagct ctcggtcgag gcgagcaagc tcggccagga ggtgggggtg
     3601 cgcacccagg tggacgtgct caacgcgcag ggcctgctgt tccaggcgca acgcgacctg
     3661 gcgcgcgcct attacgtcag catcctgagc cagctgcgtc tcaaagccag cgtcggccgg
     3721 ctgtccgagg tcgatctgga gaacgtcaac cggctgctcg ccaagtaggc ttgaacgatc
     3781 tgcgcgccga gaaggccgcg ctgcggcaag cggtgctggc gcgccgtaat gcccttcccc
     3841 atacggcacg tgccgatctg agccgccgca tcaccgctgc cctgctggcg cagccggcac
     3901 tggccacagc cggtactgtg gccgcctacc tttcgttcgg gagcgagttc gataccgccg
     3961 atttcgtcgc gcatgtgctc ggcgccggca agcgcctggt gctgccgcgg gtcaaccggg
     4021 aacggcgtcg gctcgatctg catgccgtgc aggacctgga cgccggcctc gtgccgggcg
     4081 tgtggggcat tcgcgagccg tcgcccgagc gttgcccgct ggcgacgctg c
//
LOCUS       DASPGE010000155        12750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1331233, whole genome shotgun sequence.
ACCESSION   DASPGE010000155 DASPGE010000000
VERSION     DASPGE010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12750
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331233"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VD791_04875"
     CDS             complement(<1..683)
                     /locus_tag="VD791_04875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV07338.1"
                     /translation="MQSTSWRTPAIVLTCGALILTLSMGVRQSMGLFLPPMTLEFGWG
                     RSTFAFAMALSNLIWGAFQPVFGAVADRRGAGKVLAGSALLYAAALALMPFSSSGAML
                     DLSAGLMMGLGMCGVTFGVILSVIGRAYPAERRSFALGVASAGGSFGQFVMLPFTQQL
                     IGALGWKGALFALAASLLVIVPLSAALIERHGVPGPQAQQSLGAALREAGSHAGFLYL
                     TAGFFVCGF"
     gene            complement(847..3963)
                     /locus_tag="VD791_04880"
     CDS             complement(847..3963)
                     /locus_tag="VD791_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV07339.1"
                     /translation="MVLSDISIQRPVLATVMSLLIVLLGAICFERLSVREYPNIDPPT
                     VSVRTVYKGATSQVVESVITVPIEDALSGIEGIKTIKSQSREEVSQITVTFVTGRDVD
                     AAAADVRDRVSRIAYLLPEAALDPVVAKIEADAFPIMWIALVSDRHSAMELTDYADRY
                     LTDPMKALSGVATVIIGGERKYSMRVWLDRERLAAQGLTAQDVEDALRRQNLDSPGGR
                     IESTDREFTVLAETDLKSAEQFNEMIIAEVNGYSVKLKDVGFARPGPYENRKIIRVNG
                     IDGLGLGVVKQSTANTLEVAQGVKSIIPRLKAGLPEGMNMWLAVDTSEFVEASIDAVY
                     KTLVEALVLVVLVIFLFLRSPRATLIPAVTIPVSLVGAFFFVYLMGFSINVLTMLGIV
                     LAVGLVVDDAIVVLENIHRHIEDGMSPYKAAVQGSKEIGFAVVAMTLTLAAVFTPLVF
                     MTGRTGQLFTEFAMTVAAAVLVSGFVALTLTPMMCSKLLKHHASHSPFYNLTERFFEG
                     MNDGYRGLLRATLNARWVVGIVFAGAVGLLLFLGWQIKDKAELSPIEDRGMFMMFAIA
                     PEGSTMQYTDRYMYAVEEIAKGVPEIETIFAVVAPGLERPNPVNIGIGFSVLKHWDQR
                     DRAQMEITKELTPKLFGGLPGVISFTKDPPSLGGSFLDKKLNYVLYGKTYEELQDKVG
                     KMMGKLSQYKGITGLDTDLKLNKPQLKVDIDREKAAALGVSMETIGHTLETLMGGRDV
                     TRYKREGKQYDVVVQVEDDKRRQPSDLTSIYVRGAGGDLHQLSNLVSVRETVAPKELN
                     HYNKQRAAIINGNVAEGFSLGEVLDFVDKTVKEELPADQVTDLDGESREFREAGAEAT
                     VVLVLAVIFIYLVLSAQFESFVSPLVIMFTVPLAWAGAMLAMWLNLKFGTGGTLNIYS
                     RIGLVMLVGLITKHGILIVEFSNQLRRRGMEKFEAVIEASTLRLRPILMTTAAMVLGA
                     LPLALSTGAGAEARRAIGWVIVGGLLLGTLLTLFVVPAVYSLLVRKVRVPGEEPAEKP
                     KLAPGPAH"
     gene            complement(4007..5215)
                     /locus_tag="VD791_04885"
     CDS             complement(4007..5215)
                     /locus_tag="VD791_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV07340.1"
                     /translation="MIRTVALLAALVIAASTAACGKKEEPAAGAPPKPLSSGPAASGA
                     GGPPPGLPVKAVQVTVGEVQADVSAVGSLLAEESVIIRPELDGRVVDIHFAEGQHVAK
                     GAKLVTLDPAEYRAQLAQADAQVRTDAQRYERAKELLAQKFISQEAVDLAKNNLDRSE
                     ALHSEAQVRLSKAIITAPFSGTVGLRLISPGAYVEKGTDIVRLDNISSIKLDFRVPEL
                     YAAQVRPGQQVAVRLDAFPGEQFTGRIFAMEPVVDEKTRTILLRARVPNQGLKLKPGM
                     FVRVALTLETRKNAILVPEPAIWPQGQDSFVFRVEGGKAMLTKVEIGARRPGEVEIIA
                     GLSPNDTVVTEGQMKLRDGAPVMVLPSQPAPTAGGGSSGVPPGPTAGALKPAAASTGP
                     AAQASEKKGG"
     gene            5368..6270
                     /locus_tag="VD791_04890"
     CDS             5368..6270
                     /locus_tag="VD791_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXV07341.1"
                     /translation="MKLSLDGLLVLDAIARKGSFAAAAEELHRVPSAITYTVQKLEQD
                     LGVQLFDRSGHRARLTPAGEELLKDGRHLLRAAVDIECRVQRVAKGWETELRIAHDDV
                     ISTARLCPLIAEFYAEGSGTRVRIGAEVLGGCWDALMSGRADLCIGAPAEPPLEAGYT
                     VRPLGDIDWVFAVAPAHPLAQAPQPLPTSAIMEQRVVLVADSSRNLPPRSSGIAPGPD
                     ALTVSTLQAKLEAQIAGLGVGFVPARAAAKALAEGQLLALAVEEPKPKTRLYCAWRPR
                     DAGKALKWWVAKLEEPGVRERLLA"
     gene            6301..6774
                     /locus_tag="VD791_04895"
     CDS             6301..6774
                     /locus_tag="VD791_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HXV07342.1"
                     /translation="MNIDISLADNQRKPVVDILNRLLADEVTLYVKTRNFHWNVEGAD
                     FSELHKFFEDQYEQLDEIMDEVAERARALDGYAAGSLGEFAAATRLKEAKGGRVAAKE
                     MVAQLLADHQAIIRNLRAEAAETGEKHGDAGTEDFLVGLMEQHEKMAWMLRAYLK"
     gene            complement(6788..9895)
                     /locus_tag="VD791_04900"
     CDS             complement(6788..9895)
                     /locus_tag="VD791_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV07343.1"
                     /translation="MIAFIVRQSIRYPGVVVALALTVVVYGLYVLTRANLDVFPEFSP
                     SQVVIQTEAPGLSAELVERQVTQPIENALSGTAGVEVVRSQSIPGLSVVTVVFRDGSD
                     VFRNRQVVGERLTMLANRLPRDVAQPTMTPLTSSASTVLGVGLTSQARSLMEVRTLVD
                     WTVRPHLMAVEGVADVNVFGGEVRQWQVQVEPDKLMRYGLALEDVVAAARRASGVVSA
                     GFLKTPNQHIFVNAEGQPASAGELARVMLAFKDGTPLRLGDVARVVEAPAASISAAAI
                     NGVPGVFLMIQGHLGSNTRAVTEDLERALQELAPVIAAEKVDVHPRMFRPANFIETAV
                     RNVQRDLLVGSVLVVVVLFLFLFNARTAFICTTAIPVSLLGAVIVLDYFGVGLNIMVL
                     GGLAIALGEVVDDAIIDTENIFRRLRENRLAARPLPAHQVVLDASVEVRSSVVYATFI
                     VALVFVPLLTLGGIAGKLFAPLGLAYILAILGSLIVALTLTPALCYLLLARGALSGDD
                     PPAVAWIKPRYRALLERIERYPGRVVAAVFALIAGGIALLPLFSGEFIPALREGHYII
                     HMTAVPGTAESESLRLGQRVAGAIGAIEGVQSVAQWVGRAPGGADTFGTHYSEFEVEI
                     GAVRGREQVRILREIRATLAGERGAQFPGVTFAVNTFLTERIEETITGFAAPLVINIY
                     GTDLDLLDRDAQAVATVVSGVPGARDVQLQAPPGTAQLAIRLRHERMAALGVQPGAAL
                     DAIQTAFAGAQVGQVYEGSRVVDLVVVLAPEVRERVPAVGALRLRAADGRLIQLRDVA
                     DVALTGGRYKILHASGRRLQTVTADFEGRDLASFDATVRERIAAQVRLSPGNYLVYAG
                     TARAQAQAREDLIVHSAIAGVGIFLLLYVAFNNLRNLAVTFLNLPFALIGGVLAVLLA
                     SGGWMSLGSLVGFVTLFGITLRNSIMLVSHYQYLVEVEGMPWGTETALRGASERLPSI
                     LMTALVTALALLPLALGTGEPGREIEGPMATIIVGGLITSTILNLLILPTILLQFGRF
                     GAQRR"
     gene            complement(9899..10960)
                     /locus_tag="VD791_04905"
     CDS             complement(9899..10960)
                     /locus_tag="VD791_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07344.1"
                     /translation="MRPQRSTLVIAAMGAIIAALAWAVVYLARDELQWGSEAHEEEGI
                     VTASAVGADGGFAVVTVSPESQQASGLQTAPLQAVRSQATVEIYGVVVDPRPLVEARA
                     RLMALTGEREALRVAAANAQGEYQRLKRLYEDERNVAERAVLAAQAQWLGEQARMATL
                     DQRAASIRDELRSGWGPVLAGWAQTPNAPVLDALVQQQQALVQLVFPYDVQAGGDQIA
                     LAPVGAREAQRPARFVSPSPRTDGSLPGATYFYLAGGRDLRVGVRVTGQLRLPGKARD
                     GVLMPDTAVVWHGGKAWAYVKDGDARFVRREVSTAQELPGGWFNADGFVAGDAVVVSG
                     AQLLLSEEFKFQIRNENED"
     gene            complement(10999..12417)
                     /locus_tag="VD791_04910"
     CDS             complement(10999..12417)
                     /locus_tag="VD791_04910"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXV07345.1"
                     /translation="MFPGIPRWPRPAALPTLCLLSACALQSYQPATLDPDAEAAAYMA
                     RRMDDPELQAYMLGHGHPAADWPVQRWGLTQLTLLAFYYHPDLRVARAQAAEARAQVP
                     VAAQRLPLIASPRVEHHSRETEDSDSPWSLGFAVEIPLSGAARRDALVQRATYLAEAA
                     ELEVGSTAWRVRSRVRAALADLHAARERGALLEQDIAVRSALVGLLEKRLQAGYVGAN
                     EAAAARLGLHEAQGELSAARTAAERAQGELAAALGVPLPVVRQATLDFAQLDALPAPP
                     EESEARSGALRNRIDMRQRLLEFAAADADVKLQVARQYPTVALRPGYLWDQGDSVWSL
                     ALDLLLPPAFGNAPAIEGARARRETAAQLALHGQSQIIAQTDAALATYRQSLAGATSL
                     QQASATQLARSQQTQKRFDAGYADRVELTQVRLEALAVERNAQSARVEARRALGSLED
                     ALQRPLAGGPLPVAPAPQEPQR"
     gene            12568..>12750
                     /locus_tag="VD791_04915"
     CDS             12568..>12750
                     /locus_tag="VD791_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXV07346.1"
                     /translation="MDIRNGFLETIGNTPLIRLRRCSEQTGCEILGKAEFLNPGGSVK
                     DRAALFIVQDAERSGAL"
BASE COUNT         1986 a         4568 c         4357 g         1839 t
ORIGIN      
        1 tggaagccgc atacgaaaaa gccggcggtg aggtacagga aaccggcatg cgatccggcc
       61 tcgcgcagcg ccgccccgag cgattgctgc gcctgcgggc cgggcacccc gtggcgctcg
      121 atcagcgccg ccgacagcgg cacgattacg agcaggctcg ccgccagcgc gaacagcgcg
      181 cccttccagc ccagcgcgcc gatcagctgc tgcgtgaacg gcagcatcac gaactggccg
      241 aaggagccgc cggccgaggc cacgccgagc gcgaagctgc gccgctcggc cgggtaggcg
      301 cggccgatga cgctcaggat cacgccgaag gtcacgccgc acatgcccag gcccatcatc
      361 aggccggcgc tgagatcgag catcgcgccg ctgctggaga agggcatcag cgcgagcgcc
      421 gccgcgtaca gcagggcgct gcccgcgagc accttgcccg cgccgcgccg gtcggcgacc
      481 gccccgaaca ccggctggaa ggcgccccag atcaggttcg acagcgccat ggcgaaagcg
      541 aaggtggaac ggccccagcc gaactcgagc gtcatgggcg gcaggaacag gcccatggac
      601 tggcgcacgc ccatcgacaa ggtcaggatg agcgcgccgc aggtcaggac gatcgcgggg
      661 gtgcgccagc tcgtcgattg catgctcgcc tcgcggaggg ggatggggaa ttatctcacc
      721 gcgcgggcgc gcggccagca ccccgtcgag ggagagggca cctgcggcgc ctcgcgccgg
      781 agccccctcg ctccggcccc tctcccggaa ggggagaggg gatccctgcg ctcagcgcgc
      841 cgggtctcag tgcgcggggc cgggcgccag cttcggcttt tcggccggct cttcgccggg
      901 cacgcgcacc ttgcggacca gcagcgagta gaccgcgggc accacgaaca gcgtgagcag
      961 cgtgccgagc agcaggccgc cgacgatcac ccagccgata gcccggcgcg cttcggcgcc
     1021 cgcgccggtg gacagcgcca gcggcagcgc gcccagcacc atcgccgcgg tggtcatcag
     1081 gatcggtcgc agccgcagcg tcgaggcctc gatcaccgcc tcgaacttct ccatgccgcg
     1141 gcggcgtagc tggttggaga actccacgat caggatgccg tgcttggtga tcaggccgac
     1201 cagcatcacc agcccgatgc ggctgtagat gttgagcgtg ccgccggtgc cgaacttcag
     1261 gttgagccac atggcgagca tcgcgccggc ccaggccagc ggcaccgtga acatgataac
     1321 cagcgggctc acgaagctct cgaactgcgc ggagaggacc aggtagatga agatcaccgc
     1381 caggaccagc accacggtgg cttccgcccc ggcctcgcgg aactcgcgcg actcgccgtc
     1441 gagatcggtc acctggtccg cgggcagctc ctccttcacg gtcttgtcca cgaagtcgag
     1501 cacctcgccc aggctgaaac cctccgccac gttgccgttg atgatggccg cgcgctgctt
     1561 gttgtagtgg ttcagctcct tgggcgccac cgtctcgcgc acgctgacca ggttggagag
     1621 ctgatgcaga tccccaccgg caccgcgcac gtagatcgag gtgaggtcgc tgggctggcg
     1681 gcgcttgtcg tcctccacct gcaccacgac gtcgtactgc ttgccttcgc gcttgtagcg
     1741 ggtgacgtcg cggccgccca tgagcgtctc cagcgtgtgg ccgatggtct ccatcgacac
     1801 gccgagcgcc gccgccttct cgcggtcgat gtccaccttg agctgcggct tgttcagctt
     1861 caggtcggtg tccaggccgg tgataccctt gtactgggac agcttgccca tcatcttgcc
     1921 gaccttgtcc tgcagctcct cgtaggtctt gccgtacagc acgtagttga gcttcttgtc
     1981 caggaagctg ccgcccagcg aaggcgggtc cttggtgaag gagatcacgc ccggcaggcc
     2041 gccgaacagc ttgggcgtga gctccttggt gatttccatc tgcgcgcggt cgcgctggtc
     2101 ccaatgcttg agcaccgaga agccgatgcc gatattgacc gggttgggac gctccagccc
     2161 cggcgccacc accgcgaaga tggtttcgat ctcgggcacg cccttggcga tctcctccac
     2221 cgcgtacatg tagcggtcgg tgtactgcat ggtggagcct tccggcgcga tggcgaacat
     2281 catgaacatg ccgcggtcct cgatcgggga cagctcggcc ttgtccttga tctgccagcc
     2341 caggaacaac agcagcccga cggcgccggc gaacacgatg ccgaccaccc agcgcgcatt
     2401 cagcgtcgcg cgcagcaggc cgcggtagcc gtcgttcatg ccctcgaaga agcgctcggt
     2461 gaggttgtag aacgggctgt ggctcgcatg gtgcttgagc agtttggagc acatcatggg
     2521 cgtcagcgtc agcgccacga accccgacac caggaccgcg gcggccaccg tcatcgcgaa
     2581 ctcggtgaac agctgcccgg tgcgcccggt catgaacacc agcggcgtga acacggcggc
     2641 gagcgtgagc gtcatggcaa cgaccgcgaa gccgatctcc ttgctgccct ggaccgccgc
     2701 cttgtagggc gacatgccgt cttcgatgtg gcggtggatg ttctccagca cgacgatggc
     2761 gtcgtccacc accaggccca ccgccagcac gatgccgagc atggtgagca cgttgatgga
     2821 gaagcccatc aggtacacga agaagaacgc gccgaccagc gacaccggga tggtgaccgc
     2881 cgggatcagg gtcgcgcgcg gactgcgcag gaacaggaag atcaccagca ccaccaggac
     2941 cagcgcttcg accagcgtct tgtacaccgc gtcgatggag gcctcgacga attccgaggt
     3001 gtccaccgcc aaccacatgt tcatgccttc gggcaggccg gccttgaggc gcgggatgat
     3061 gctcttgacg ccctgggcca cctccagcgt gttggcggtg gactgcttca ccacccccag
     3121 gccgaggccg tcgatgccgt tgacacggat gatcttgcgg ttctcgtagg gccccgggcg
     3181 cgcgaagccc acgtccttga gcttgaccga gtagccgttc acctccgcga tgatcatctc
     3241 gttgaactgc tcggcgctct tcaggtcggt ctcggccagc acggtgaact cgcggtccgt
     3301 gctctcgata cgcccgcccg gcgagtcgag gttctgccgg cgcagcgcgt cctccacgtc
     3361 ctgcgcggtc aggccctggg cggcgagccg ctcgcggtcg agccacacgc gcatcgagta
     3421 cttgcgctcc ccgccgatga tcacagtcgc cacgcccgac agcgccttca tcggatcggt
     3481 caggtagcgg tccgcgtagt cggtcagctc cattgcgctg tgccggtcgc tgaccagcgc
     3541 gatccacatg atcggaaagg cgtcggcttc gatcttggcc accaccgggt cgagcgccgc
     3601 ctccggcagg agataggcga tgcgcgagac gcggtcgcgc acgtcggccg ctgccgcatc
     3661 cacgtcgcgg ccggtgacga aggtcaccgt gatctggctg acttcctcgc ggctctgcga
     3721 cttgatggtc ttgatgccct cgatgccgga cagcgcgtcc tcgatcggca cggtgatcac
     3781 gctctccacc acctgcgagg tggcgccttt gtacaccgtg cgcaccgaga ccgtgggcgg
     3841 atcgatgttg ggatactcgc gcaccgacag gcgctcgaag cagatcgcgc cgagcagcac
     3901 gatcagcagg ctcatcaccg tagccaggac cgggcgctgg atggagatgt cggatagaac
     3961 catgattgac ccccccgtga cggggaaatg gaaaccgggc ggccgctcag ccgcccttct
     4021 tttcggacgc ttgggccgcg ggcccggtgc tcgcggcagc gggcttgagc gcgcctgcgg
     4081 taggaccggg cggcacgccg gaagatccgc cgccggcggt gggcgccggc tgcgagggca
     4141 gcaccatgac cggcgcgccg tcccggagct tcatctggcc ttcggttacc acggtgtcgt
     4201 tgggcgacag gcccgctatg atctcgacct cgccgggccg gcgggcgccg atttccacct
     4261 tggtgagcat cgccttgccg ccttctaccc ggaacacgaa gctgtcctgg ccctgcggcc
     4321 agatggccgg ctcgggcacc aggatcgcgt tcttgcgcgt ctccagcgtc aaggccacgc
     4381 gcacgaacat gccgggcttg agtttcaggc cctggttggg cacgcgggcg cgcagcagga
     4441 tggtgcgggt cttctcgtcc accaccggct ccatggcgaa gatgcggccg gtgaactgct
     4501 caccggggaa ggcgtccagg cgcactgcca cctgctggcc cggacgcacc tgcgccgcgt
     4561 acagttccgg cacccggaag tcgagcttga tcgagctgat gttgtccagg cgcacgatgt
     4621 ccgtgccctt ctccacgtag gcgccggggc tgatcaggcg caggccaacg gtgccggaga
     4681 acggcgcggt aatgatggcc ttggacaggc gcacctgcgc ttcgctgtgc agggcctccg
     4741 agcggtccag gttgttcttg gcgagatcga ccgcttcctg gctgatgaac ttctgggcca
     4801 acaactcctt ggcgcgctcg tagcgctggg catcggtgcg cacctgcgca tcggcctggg
     4861 cgagctgcgc ccggtactcc gccgggtcca gcgtcaccag cttggcgccc ttcgccacgt
     4921 gctggccttc ggcgaagtga atgtccacca cgcgaccatc cagctcgggc cggatgatga
     4981 ccgattcctc cgccagcagg ctgccgaccg cactcacgtc ggcctgcacc tcgcccaccg
     5041 tcacctgcac ggccttgacc ggcaggcccg ggggcggtcc gcccgccccg cttgccgccg
     5101 ggcctgagga taggggcttg ggcggcgcgc cggccgcggg ctcctccttc ttgccacagg
     5161 ccgcggtcga ggcggcgatg accagcgccg cgagtagcgc tacagtacgg atcacggatg
     5221 tattcaatgc cgggtacctc gggtagccgc tccgggaggc gggggctgtt ctggacgctc
     5281 ttttggcctt atggagcgat gaaccgggga ttctagcctg atttacgggt cgctggccac
     5341 aattaccgaa cgctgcgtct tgttttcatg aagctctctc tggacggtct gctggtgctg
     5401 gatgccatcg cccgcaaggg cagtttcgcc gccgcggcgg aggagctgca ccgcgtgccc
     5461 tcggcgatca cttacacggt gcagaagctg gagcaggatc tgggcgtcca gctcttcgac
     5521 cgcagcgggc accgcgcccg cctcaccccc gcgggcgagg agctgctcaa ggatggccgc
     5581 cacctcctgc gcgccgcggt ggacatcgaa tgccgcgtgc agcgcgtggc caagggctgg
     5641 gaaacggagc tgcgcatcgc ccacgacgac gtgatctcga ccgcgcggct gtgcccgctg
     5701 atcgccgagt tctacgcgga agggtcgggc acgcgcgtgc gcatcggcgc cgaggtgctg
     5761 ggcggctgct gggacgcgct catgtccggg cgcgcggacc tgtgcatcgg cgcgccggcc
     5821 gaaccgccgc tcgaggccgg ctacaccgtg cgcccgctcg gcgacatcga ctgggtgttc
     5881 gcggtggcgc ccgcgcatcc gctggcgcag gcgccgcaac cgctgccgac ctcggcgatc
     5941 atggagcagc gcgtggtgct ggtggcggac agctccagga acctgccgcc gcgcagctcc
     6001 ggcatcgcgc cgggccccga cgcgctcacc gtctccaccc tgcaggcgaa gctcgaggcg
     6061 cagatcgccg ggctcggcgt gggtttcgtg cccgcgcgcg cggcggcgaa agcgctcgcc
     6121 gagggccagc tgctcgcgct cgccgtcgaa gagccgaagc ccaagacgcg cctgtactgc
     6181 gcctggcgcc cgcgcgacgc gggcaaggcg ctcaagtggt gggtggccaa gctcgaggag
     6241 ccgggggtgc gcgagcggct gctggcgtga cattccgatc gacaaccaca ggagatcccc
     6301 gtgaacatcg atatcagcct cgccgacaac cagcgcaaac cggtcgtcga catcctcaat
     6361 cgcctgctcg ccgacgaggt cacgttgtac gtgaagacgc gcaacttcca ctggaacgtg
     6421 gagggcgcgg acttcagcga gctgcacaag ttcttcgagg accagtacga gcagctcgac
     6481 gagatcatgg acgaggtggc ggagcgcgcg cgcgcgctcg acggctacgc cgccggctcg
     6541 ctgggcgagt tcgccgccgc cacccggttg aaggaagcga agggcggccg cgtcgcggcc
     6601 aaggagatgg tggcgcagct gctcgccgat caccaggcga tcatccgcaa tctgcgcgcc
     6661 gaggccgcgg aaacgggcga aaagcacggc gacgcgggca ccgaggactt cctcgtcggc
     6721 ctcatggagc agcacgagaa aatggcgtgg atgctgcgcg cctacttgaa gtagagcacc
     6781 ctcccgctca ccggcgctgc gcgccgaagc gcccgaactg cagcaggatg gtgggcagga
     6841 tcagcaggtt gagaatggtc gaggtgatca ggccgccgac gatgatggtg gccatcgggc
     6901 cttcgatctc gcgccccggc tcgccggtgc cgagcgccag gggcagcaac gccagcgcgg
     6961 tgaccagcgc cgtcatcaga atcgacggca ggcgctcgct ggcgccgcgc agcgcggtct
     7021 cggtgcccca gggcatgcct tcgacctcga ccagatactg gtagtgcgac accagcatga
     7081 tcgagttgcg cagcgtgatg ccgaacagcg tgacgaagcc caccagcgag ccgagcgaca
     7141 tccagccgcc gctcgccagg aggacggcga gcaccccgcc gatcagcgcg aacggcaggt
     7201 tcaggaaggt gaccgccagg ttgcgcagat tgttgaaggc cacgtacagc agcaggaaga
     7261 tgccgacgcc ggcgatcgcg gagtgcacaa tcaagtcctc gcgcgcctgc gcttgcgcgc
     7321 gcgccgtgcc cgcatagacc aggtagttgc cgggcgacaa gcgcacttgc gcggcgatcc
     7381 gctcgcgcac ggtcgcgtcg aagctcgcca ggtcgcggcc ctcgaagtcg gcggtgaccg
     7441 tctgcagccg ccgcccgctc gcatgcagga tcttgtagcg cccgccggtg agggcgacat
     7501 cggccacgtc ccgcagctgg atcaggcgtc cgtcggccgc gcgcaggcgc agagcgccga
     7561 ccgccgggac ccgctcgcga acctccggcg cgagcaccac gaccaggtcc accacgcggc
     7621 tgccttcgta cacctgcccg acctgcgcgc cggcgaatgc ggtctggatc gcgtccagcg
     7681 ccgcgccggg ctggacgccc agcgcggcca tgcgctcgtg gcgtagccga atggcgagct
     7741 gcgccgtgcc gggcggcgcc tgcaactgca cgtcgcgcgc gccgggcacg ccgctcacca
     7801 cggtggccac cgcctgggca tcgcggtcga gcaggtccag atcggtgccg tagatgttga
     7861 tcaccagcgg cgccgcgaag ccggtgatgg tctcctcgat gcgctcggtc aggaaggtgt
     7921 tgaccgcgaa ggtgacgccc gggaactgcg ctccgcgctc gccggcgagg gtggcgcgga
     7981 tctcgcgcag gatgcgcacc tgctcgcgcc cgcgcaccgc gccgatctcc acctcgaatt
     8041 cgctgtagtg cgtgccgaag gtgtcggcgc cgccgggcgc gcgtccgacc cactgcgcca
     8101 ccgactgcac gccttcgatc gcgccgatgg ccccggccac gcgctggccg agccgcagcg
     8161 attccgactc tgcggtgccc ggcaccgccg tcatgtggat gatgtagtgc ccctcgcgca
     8221 gcgccgggat gaactcgccc gagaacagcg gcagcagggc gatgccgccg gcgatgagcg
     8281 cgaagaccgc ggccaccacc cggcccggat agcgctcgat gcgctccagc agcgcccggt
     8341 agcgcggctt gatccaggcc acggcgggcg ggtcgtcgcc gctcagcgcg ccgcgcgcga
     8401 gcagcaggta gcacagcgcc ggggtgagcg tgagcgccac gatcagcgag cccaggatcg
     8461 ccaggatgta ggccagcccg agcggcgcga acagcttgcc ggcgatgccg cccagcgtga
     8521 gaagcggcac gaacaccagc gcgacgatga aggtggcgta caccacggag ctgcgcacct
     8581 ccaccgaagc gtccagcacc acctggtgcg cgggcagcgg ccgggccgcc agccggttct
     8641 cgcgcaggcg ccggaagatg ttctcggtgt cgatgatggc gtcgtccacc acttcgccga
     8701 gcgcgatggc cagcccgccc agcaccatga tgttcaggcc cacgccgaag taatccagca
     8761 cgatcactgc acccagcagc gacaccggga tggcggtggt gcagatgaag gcggtgcgcg
     8821 cgttgaacaa gaacaagaac aggacaacga ccaccagcac cgagcccacc agcaggtcgc
     8881 gctgcacgtt gcgcaccgcc gtctcgatga agttggcggg ccggaacatg cgcggatgca
     8941 cgtccacttt ctccgcggcg atgaccggcg ccagctcctg cagggcgcgc tccaggtcct
     9001 cggtgaccgc gcgcgtgttg ctgcccaggt gcccctggat catcaggaac acgccgggca
     9061 cgccgttgat cgccgccgcg ctgatggacg cagccggcgc ctccaccacc cgcgccacgt
     9121 cgcccagccg cagcggcgtg ccgtccttga aggcgagcat gacgcgcgcc agctcgccgg
     9181 cggaggccgg ctggccctcg gcgttgacga agatgtgctg gttcggggtc ttcaggaagc
     9241 ccgccgacac cacgccgctc gcgcggcgcg ccgcggcgac cacgtcctcc agcgccaggc
     9301 cgtagcgcat cagcttgtcg ggctccacct gcacctgcca ctggcgcact tcgccgccga
     9361 acacgttgac gtcggccacg ccctccacag ccatcaggtg cggccgcacc gtccagtcca
     9421 ccagcgtgcg gacctccatc agcgagcgcg cctgcgacgt gagacccacg cccaacacgg
     9481 tgctcgccga ggacgtgagc ggcgtcatgg tcggttgcgc cacgtcgcgc ggaagccggt
     9541 tggcgagcat cgtcaagcgc tcgcccacca cctgccggtt gcggaacacg tccgagccgt
     9601 cgcggaacac cacggtcacc accgacagcc cgggaatcga ctgcgagcgc accacctcga
     9661 cgcccgccgt tcccgacagc gcgttctcga tcggctgggt gacctggcgc tcgaccagct
     9721 ccgccgacaa gccgggcgcc tcggtctgaa tcaccacctg gctgggcgag aactccggaa
     9781 acacgtccag gttcgcgcgc gtgagcacgt acaggccgta cacgacgacc gtcaacgcca
     9841 gggcgacgac cacgcccggg tagcggatcg actggcggac gatgaaggcg atcaactact
     9901 agtcctcgtt ctcgttccgg atctggaact tgaactcctc ggacagcagc agctgcgcgc
     9961 cgctgacgac caccgcgtca ccggcgacga agccatcggc attgaaccag cccccgggca
    10021 gctcctgcgc ggtggacacc tcgcggcgca cgaagcgtgc atcgccgtcc ttgacgtagg
    10081 cccaggcctt gccgccgtgc cacaccacgg ccgtgtcggg catcagcacg ccgtcgcgcg
    10141 ccttgccggg caggcgcagc tgacccgtca cgcgcacgcc cacgcgcaga tcgcggccgc
    10201 cggccagata gaaatacgtg gcgccgggca acgagccgtc ggtgcgcggc gagggcgaga
    10261 cgaagcgcgc agggcgctgc gcttcgcgcg caccgaccgg cgccaatgcg atctggtcgc
    10321 cgcccgcctg cacgtcgtag ggaaacacca gctgcaccag cgcctgctgc tgctgcacca
    10381 gtgcgtcgag caccggggcg ttcggcgtct gggcccagcc cgcgagcacc ggcccccagc
    10441 ccgagcgcag ctcgtcccgg atcgacgcag cccgctggtc gagcgtggcc atgcgtgcct
    10501 gctcgcccag ccactgcgcc tgcgccgcca gcaccgcgcg ctcggccacg ttgcgctcgt
    10561 cctcgtagag gcgcttgagc cgctgatact cgccctgcgc gttggccgcc gccacgcgca
    10621 gcgcctcgcg ctcgccggtg agcgccatca ggcgggcgcg cgcctccacc agcggccgcg
    10681 ggtcgaccac cacgccatag atctcgactg tcgcctgtga gcgcacggcc tgcagcggcg
    10741 cggtctgcag cccgctcgcc tgctggctct cggggctgac cgtcaccacg gcgaagcctc
    10801 cgtccgcacc cactgccgag gcggtgacga tcccctcctc ctcgtgcgcc tccgaccccc
    10861 actgcagctc gtcgcgcgcc aggtagacca cggcccacgc cagcgcggcg atgatcgcgc
    10921 ccatcgccgc gatgaccagc gtgctgcgct ggggtctcat cgctggggtc tcatcgctgg
    10981 ggcctcatcg ctggggcctc accgctgcgg ctcctgcggc gcgggcgcga cgggcagggg
    11041 accgccggcg agcgggcgct gcagcgcatc ctcgaggctg cccagcgccc gccgcgcctc
    11101 gacgcgggcg ctctgcgcat tgcgctcgac ggccaacgcc tccaggcgca cctgggtcag
    11161 ctcgacgcga tcggcgtagc ccgcgtcgaa ccgcttctgc gtctgctggc tgcgggcgag
    11221 ctgcgtcgcg ctcgcctgct gcaaggaggt ggcgcccgcc agagactgcc ggtacgtggc
    11281 gagcgcggca tcggtctgcg cgatgatctg cgattggcca tgcagcgcga gctgcgcggc
    11341 ggtctcacgc cgggcgcgcg cgccctcgat ggcgggtgca ttgccaaacg ccggcggcag
    11401 cagcaggtcg agcgccagcg accagacgct gtcgccctgg tcccacagat agcccgggcg
    11461 cagcgcaacg gtgggatatt gtcgtgccac ctgcagcttg acgtcggcat ccgccgccgc
    11521 gaactcgagc agccgctggc gcatgtcgat gcggttgcgc agcgccccgc tgcgcgcctc
    11581 gctttcctcg ggcggcgccg gcagcgcatc gagctgcgcg aagtcgagcg tcgcctggcg
    11641 cacgaccgga agaggcacgc ccagggccgc ggccagttcg ccctgcgcgc gctccgccgc
    11701 ggtgcgcgcc gccgacagct ccccctgcgc ttcatgcagg cccaggcgtg ccgcggcggc
    11761 ttcgttggcg cccacgtagc cggcctgcag gcgtttctcc agcaggccca ccagcgcgct
    11821 gcgcaccgcg atgtcctgct cgagcagcgc gccgcgctcc cgggccgcgt gcagatcggc
    11881 gagcgcggcg cgcacccggc tgcgcacgcg ccaggcggtg ctgcccactt ccagctcggc
    11941 ggcctcggcc agataggtcg cacgctgtac cagcgcgtcg cggcgggcgg ccccgctcag
    12001 cgggatctcg actgcgaagc ccaggctcca gggactgtcg ctgtcctccg tctcgcgact
    12061 gtggtgctcg acgcgcgggc tcgcgatcag gggcagccgt tgcgccgcga ccggtacctg
    12121 cgcgcgggcc tcggcggcct gcgcccgcgc cacgcgcagg tccggatggt agtagaacgc
    12181 gagcagcgtg agctgcgtca atccccagcg ctgcaccggc cagtccgccg cgggatgccc
    12241 gtggccgagc atgtacgcct gcagctcggg gtcatccatg cgccgcgcca tgtaggctgc
    12301 ggcctccgcg tcggggtcga gcgtggccgg ctgatagctc tgcagcgcgc aggccgacag
    12361 cagacagagc gttggcagcg ccgccggccg cggccaacgt gggattccag ggaacatggc
    12421 cggcggagtg tatcgcgcgg ctcgggcaag cgcctactgc gccctcaagg ggcgacttaa
    12481 aaaccactgg agcggcttta gaggcacgct caacaaatcg tgcgcgggct cgcggataat
    12541 aggcgctttt cgcatctcgc caaggccatg gacatccgca acggcttcct cgagaccatc
    12601 ggcaacacac cgctcatccg cctgcgccgc tgctccgagc agaccggctg cgagatcctg
    12661 ggcaaggccg agttcctcaa ccccggcggg tcggtgaagg accgcgccgc cctgttcatc
    12721 gtgcaagatg cggagcgcag cggcgcgctg
//
LOCUS       DASPGE010000156        10240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_864134, whole genome shotgun sequence.
ACCESSION   DASPGE010000156 DASPGE010000000
VERSION     DASPGE010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10240
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864134"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VD791_04920"
     CDS             complement(<1..1055)
                     /locus_tag="VD791_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXV07347.1"
                     /translation="MDDLERFDRRVGMIALAALAVGCLLVLRPFVSSVLWALVLSYST
                     WPVFLRVRHLLRGHSAAAALTMTVLLAVVIVVPVMVLGFSLGDNVAPVVDAGRRVMVE
                     GLPDPPHWVASVPLVGGQLEAYLREMAHDSAKLMSVLAQLLDPLKSWLVSGGLKLGEG
                     LLYLVLSVVLAFFFYRDGDAVSRGLDSALTRIAGARAARLKQVAGDTVASVVYGILGT
                     GLAQGVMAALGLRIAGVPGSLLWGTLTFFLSVVPVGPPLVWISATVWLVFQGSIGWAV
                     FMAVWGFFGISGIDNVVKPYLISRGSRLPFILTLLGVLGGALAFGIVGVFLGPTLLAV
                     GFRLLQEWIARDSPPALL"
     gene            complement(1101..1967)
                     /locus_tag="VD791_04925"
     CDS             complement(1101..1967)
                     /locus_tag="VD791_04925"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HXV07348.1"
                     /translation="MTRAEPNIRPRRSLLFVPALRPDRFAKALGSGADIVCVDMEDAV
                     ALPRKDEGRALALQLFRDAGHPQVEKMARINGLSTVHGLKDLQALIEMQAPPPAIMIP
                     KVKSAAEIQLIEALLSAGAARAIRFCVIVETNQGLEHALDIARASPRIDSMILGAVDM
                     SAELRCQKAWEPLLYTRSRLVHAAASAGIDLLDVPWLNLDDPQGLQAEAQACQRLGFT
                     GKASIHPDQIPIINAAFTPDASTIAKARKVCAAFEQADTGLVVVDNELIELPVVRSYY
                     RVLAIADRLSKT"
     gene            complement(2038..3567)
                     /locus_tag="VD791_04930"
     CDS             complement(2038..3567)
                     /locus_tag="VD791_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HXV07349.1"
                     /translation="MPGRPRHPALSTGSVMLGAFLYSIDWSIAAVALPHMQGTFSATH
                     DQIAWVITSYIVTSAIVMPTGGWLSTRFGRKRVFVWALVGFTAASVLCGAANTLTGEV
                     LARILQGASGAFLIPLSQAITLDTYPPEQHGKAMALWGTGAVMGTMMGPTVGGYLTEY
                     LSWRYIFYINVPFGLLALLGVLAFVPETQREPGRRLDWFGFLTLALGIGALQMMLDRG
                     QRLDWFESEEIVLLACLGALGLYLFAVHSATARQPFLDPRLIANRAFFVSLLLISVYG
                     FLTVPVMVLMPAFLEYLRGYTIDTIGLLQSSRGLGLLIALFVSGRIADKVDARIPIAF
                     GLLCLIVSGSEMSSWTAEVGPWPIVWTGFLQGVGGGIMLVPIQVIAFAALGAQQRTEA
                     AAVFNLVRSVWSSVGVSVMVTLFVRESSMGRARLVEHITPYSQALRFIDPASGLDLHT
                     EHGLAVIERQVELQAAMLGYGAVFLLLAVVACAALPLLLLMGRQTRLKRSDPEALVIG
                     E"
     gene            complement(3579..4709)
                     /locus_tag="VD791_04935"
     CDS             complement(3579..4709)
                     /locus_tag="VD791_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HXV07350.1"
                     /translation="MSMAPVVGNAVIAVRRVTRWLLLFGVPFAAILVGLYVYAHGGRY
                     EETENAYVKADIIAVSAEVSGRVVEVAARDNEPVAAGAPLFRLDAKPFEIELARARAQ
                     MDVVRTEVQALRAEFRETLLESTEARERIDFLTRQLERQERLKEKGMSRIEAYDEARH
                     HLEVARARLASVEERINRVRASLAGDPQLPAERHPRYVEAKTAYDAAAIDLARTQIKA
                     PAAGVVSNMKLQPGEHVEKGAPIFSLIRSGEVWIEANFKETQLTHMREGQPASVVADA
                     YPDHEWRALVAAIAPATGAEFAVLPPQNATGNWVKVVQRIPVLIRVEASTGQPPLRAG
                     MTVAVAVDIGRSRGLPRVVQRLIDKGYLPRFLEPAPALAHSE"
     gene            complement(4706..5182)
                     /locus_tag="VD791_04940"
     CDS             complement(4706..5182)
                     /locus_tag="VD791_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXV07351.1"
                     /translation="MFREDLSRNFGFILHDVARLLRTTFDRRVKALGFTRSQWWVLTH
                     LFRNDGITQSELADILEVEKPTLGRLLDRLEAKGWVRREADAADRRVKRVFLTEEIEP
                     ALRAMRAAAAEMRRDALAGLSAEEQQRFVDTLLAVKANLNGSDNGVARNGARGRKR"
     gene            5354..6565
                     /locus_tag="VD791_04945"
     CDS             5354..6565
                     /locus_tag="VD791_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXV07352.1"
                     /translation="MKVTAIETINLDEFPNVLWVQVHTDEGIVGLGETFYAVEPVIAH
                     IHQTIAPYLLGKSPLTIDAHSRHVLYNYLGFKSVGAEMRAASAVDIALWDILGQVSNQ
                     PIYQLLGGASRDKVRAYNTCAGYQYVRAKSEQGTANFGMPGDSGKYQPYEDLVGFMQY
                     ADELALSLLEEGYTGMKIWPFDEYAEASNGTYISSADLKKAMRPFEKIRKAAGDRMDI
                     HVEFHSLWNLPTAIKIAKALEQFDPFWYEDPIKMDNLDAIAEYARRTDVWVTASETLG
                     TRWAFRDLFEKRAVSVCMFDVGWTGGVSESKKIATMAEAYQLPVAPHDCTGPVLLTAS
                     VHLSMNCPNTLVQEVVRAFYSDWYGKLVTQLPPLEKGYISAPNGPGLGTRLQPDVLKR
                     KDARIRRSSLA"
     gene            6580..7110
                     /locus_tag="VD791_04950"
     CDS             6580..7110
                     /locus_tag="VD791_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXV07353.1"
                     /translation="MPRIPYQPEDLREPAAVVEAIRQRRGGRLYHLDRLLLHSPPFAA
                     AWNGFMREVRTLSIPPRLREIAICAVACLNRAEYEFHHHAPLLLAAGGTAEQVEALRR
                     LEQGGAQAPVFDSAERAVIRLTTEMTRAVQVGDDTFAAVRAVLPSEQQAVELVGVIAA
                     YNMVSRFLVALGVEPE"
     gene            7401..7883
                     /locus_tag="VD791_04955"
     CDS             7401..7883
                     /locus_tag="VD791_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07354.1"
                     /translation="MRALISALAAAGLLSLALPTFAQDTVKSIPDANKPGRQAEDDTK
                     KAVKSKPDLSKPGRTAEEDKAVKSAGPSNKPGRTAEEDKAIKAGGASNKPGRTAEEDK
                     AVKSAGPSTKPGRTAEEDKAVKAGGASDKPGRTAEEEAKAVKSAGPSDKPGRTAEEKK
                     "
     gene            complement(7975..8598)
                     /locus_tag="VD791_04960"
     CDS             complement(7975..8598)
                     /locus_tag="VD791_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXV07355.1"
                     /translation="MKILHADDHPMFREGLRFFLQLLDPQVTILEASNFQAALDKLAL
                     EGPVDLLLLDLQMPGSGELDGFFAIRRRYPALPVVIVSGVSDTRIIRTLLDGGARGYI
                     PKFASSELLMEGLRTVLRGQVFVPEGLFLPASQAPGGNGEATLTDRQLQILPLLADGM
                     ANKQIAGALGVTEGTVKQHLKDLFKRLNARNRTQAVNEARRLGLLRK"
     gene            complement(8663..9817)
                     /locus_tag="VD791_04965"
     CDS             complement(8663..9817)
                     /locus_tag="VD791_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HXV07356.1"
                     /translation="MPLATELRGATLLIVDDKPQNLRLISDFLAEQGFELMLTRNGAQ
                     ALERARLAAPELVLLDLRMPDMDGFEVCRRLKADPATAALPVIFMTAVDDIAHKVEGF
                     ALGAVDYITKPIQREELLARIQHHLQLHRLQQELYSKTQDLALKNAELEAYAHTIAHS
                     LKTPLAAAARFLEILHKFKSANLSEEQRHLLQQAQAALAASASAVEALLLLSTVSQER
                     VELHPLDMSALVGQALNQLAGLQASTRAAVQLPKSWPAALGYAPWVGEVWMNLLSNAL
                     KYSGSPPRLDLGATLEGTQARFWVRDNGQPLSEEQRRRVFTPFTRLHQERAEGHGLGL
                     ATVERIISRLGGSIAVQPAPGGGNEFSFTLPSAAAGRGARREVSNRAAAG"
     gene            complement(9801..>10240)
                     /locus_tag="VD791_04970"
     CDS             complement(9801..>10240)
                     /locus_tag="VD791_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07357.1"
                     /translation="LPGLERVPLVAASASTADLARAEADPATFAVCLRKPFQTADLLD
                     AIERVLGLSWRYGEAESAEPEDGREGRMVAPPAPVLENLLDLARLGKLVRVEQIALEL
                     EQGDPRYGPFARRLYGLARRFDEERLVGLLQEALEAHHDAVSD"
BASE COUNT         1733 a         3587 c         3440 g         1480 t
ORIGIN      
        1 agcagcgccg gcggcgaatc gcgcgcgatc cactcctgca gcagccggaa gccgacggcg
       61 agcaacgtcg ggccgagaaa cacgccgacg atgccgaagg cgagcgcgcc gccgagcacg
      121 cccagcagcg tcaggatgaa aggcaggcgg ctgccgcggc tgatgagata gggcttgacc
      181 acgttgtcga tgccgctgat gccgaagaag ccccacacgg ccatgaacac cgcccagccg
      241 atggacccct ggaacaccag ccatacggtg gccgagatcc agaccagagg cgggcccacc
      301 gggacgaccg agagaaagaa cgtcagcgtg ccccacagca gcgagccggg aacccccgcg
      361 atcctcaggc cgagcgcggc catgacgcct tgcgcgagcc cggtgccgag gatcccgtac
      421 acgacgctgg ccacggtgtc gccggcgacc tgcttcaagc gcgcggcgcg cgccccggcg
      481 atgcgcgtca gcgccgaatc cagcccgcgc gacaccgcgt cgccgtcgcg gtagaagaag
      541 aacgccagca ccacgctcag caccaggtag agcagcccct cgcccagctt gaggccgccg
      601 ctcaccagcc aggacttgag cgggtccagc agctgggcga gcaccgacat cagcttcgcg
      661 ctgtcgtgcg ccatctcgcg caggtaggcc tcgagctgcc cgccgaccag cggcacgctc
      721 gcgacccagt ggggcgggtc gggcaggcct tccaccatga cgcgccggcc ggcgtcgacc
      781 accggcgcca cgttgtcgcc gaggctgaac cccagcacca tcaccggcac cacgatcacc
      841 acggccagca gcacggtcat cgtcagagcc gccgccgcgc tgtgcccgcg cagcagatgg
      901 cgcacgcgca ggaacaccgg ccaggtgctg tagctcagca ccagcgccca cagcaccgac
      961 gatacgaacg gccgcaacac cagcaggcag ccgaccgcga gcgccgccag cgcgatcatg
     1021 ccgacgcggc ggtcgaaacg ttcgaggtcg tccatgtgcg agatcctgcg gacgcggcgt
     1081 gaggagggtc gcaggccgcg tcaggttttc gacaatctgt ccgcgatcgc cagcacgcga
     1141 taataggagc gcaccaccgg cagctcgatc agctcgttgt ccaccaccac cagcccggtg
     1201 tcggcttgct cgaacgcggc gcacaccttg cgcgccttcg cgatcgtgct cgcgtcgggc
     1261 gtgaaggcgg cgttgatgat cgggatctga tcggggtgga tcgaggcctt gccggtgaag
     1321 cccaggcgct ggcacgcctg cgcctcggcc tgcagcccct gggggtcgtc gaggttgagc
     1381 cagggcacgt cgagcagatc gatgcccgcg ctcgccgccg cgtgcaccag acgcgagcgc
     1441 gtgtagagca gcggctccca cgccttctgg cagcgcagct ccgccgacat gtccaccgcg
     1501 cccaggatca tcgagtcgat gcgcgggctg gcccgcgcga tgtcgagcgc atgctccagg
     1561 ccctgattgg tctcgacgat cacgcagaag cggatcgcgc gcgcggctcc ggcggacagc
     1621 aacgcctcaa tcagctggat ctccgcggcc gacttgacct tcgggatcat gatggcgggg
     1681 ggcggcgctt gcatctcgat gagcgcctgc aggtctttca agccgtgcac cgtggacagg
     1741 ccgttgatgc gcgccatctt ctccacttgc ggatggcccg cgtcgcggaa cagctgcagg
     1801 gccagcgcgc gcccctcgtc ctttcgcggc agcgcaaccg cgtcctccat gtccacgcac
     1861 acgatgtccg cccccgaacc gagcgccttg gcgaagcgat ccgggcgcaa tgcgggcacg
     1921 aacagcaggc tgcggcgggg acggatgttc ggttcggcac gggtcatgta cgcggctccg
     1981 tcggaaaacg tcatgctagc tcgctgtttc gcacgccgcc aggcgcgcga agcctcgcta
     2041 ctcgccgatc accagcgctt cggggtcgct gcgcttgagg cgggtctgcc ggcccatcaa
     2101 cagcaggagc ggcagagcgg cgcaggcgac gaccgcgagg agcaggaaca ccgcaccgta
     2161 gcccagcatc gccgcctgca gctcgacctg gcgctcgatg accgccaggc cgtgctccgt
     2221 gtggaggtcg agaccactgg ccggatcgat gaagcgcagc gcctggctgt agggcgtgat
     2281 gtgctcgacc agccgcgcgc ggcccatgct gctctcgcgc acgaacaagg tgaccatcac
     2341 ggagacgccg acgctgctcc atacgctgcg caccaggttg aacaccgccg ccgcctcggt
     2401 gcgttgctgc gcccccagcg ccgcgaaggc gatcacctgg atcggcacca gcatgattcc
     2461 gccgcccaca ccctgcagga aaccggtcca gacgatcggc cacggcccga cttccgccgt
     2521 ccagctcgac atctcgctgc cggaaacgat gaggcacagc agaccgaagg cgatcgggat
     2581 gcgcgcgtcc accttgtccg cgatgcggcc gctcacgaac agcgcgatca gcaatccgag
     2641 gcccctggat gactgcagca gtccgatggt atcgatggtg tagccgcgca ggtactcgag
     2701 aaacgccggc atgagcacca tgacgggcac ggtgagaaag ccgtagaccg agatcagcag
     2761 caggctcacg aagaacgcgc ggttcgcgat caggcgcgga tcgaggaacg gctggcgggc
     2821 ggtcgcgctg tgcaccgcga acagatacag ccccagggct ccgaggcagg ccagcagcac
     2881 gatctcctcc gattcgaacc aatccaggcg ctggccgcgg tcgagcatca tctgcagggc
     2941 gccgatgccc agggcgagcg tcaggaagcc gaaccagtcg agccggcgcc cgggctcgcg
     3001 ctgcgtctcc ggcacgaacg ccagcacgcc cagcagcgcg agcaggccga acggcacatt
     3061 gatgtagaag atgtagcgcc aggagagata ctcggtgagg tagccgccca cggtgggacc
     3121 catcatggtc cccatcaccg ccccggtgcc ccaaagcgcc atggccttgc cgtgctgctc
     3181 cggcggataa gtgtcgagcg tgatcgcctg cgagagcgga atcaggaagg cgccgctcgc
     3241 gccctgcagg atgcgcgcca gcacttcgcc cgtgagggta ttggccgcgc cgcacagcac
     3301 cgaggccgcg gtgaatccga ccagggccca gacgaacacg cgcttgcgcc cgaagcgcgt
     3361 gctgagccag ccgccggtag gcatcacgat ggccgaggtg acgatgtacg aggtgatgac
     3421 ccaggcgatc tggtcgtgcg tcgccgagaa cgtgccctgc atgtgcggca gggccacggc
     3481 cgcgatgctc cagtcgatcg agtacaggaa cgcccccagc atcaccgagc cggtgctgag
     3541 cgcagggtgc cgcggccggc ctggcacggg cgggctcact actcgctgtg cgcgagcgct
     3601 ggggccggct ccaggaagcg cggcaggtag cccttgtcga tcagccgctg gaccacccgc
     3661 ggcaggccgc ggctgcggcc gatgtccacg gccaccgcca cggtcatgcc ggcacgcaac
     3721 ggcggttgcc cggtggaggc ttcgacgcgg atgagcaccg gaatgcgctg caccaccttt
     3781 acccagttgc cggtcgcgtt ctgcggcggc agcacggcga actccgcgcc ggtggccggt
     3841 gcgatcgcgg ccaccagcgc gcgccactcg tggtccggat aggcgtcggc caccacgctg
     3901 gccggctggc cttcgcgcat gtgggtgagc tgggtctcct tgaagttggc ttcgatccag
     3961 acttcaccgc tgcggatcag gctgaagatg ggcgcgccct tctccacgtg ctcgccgggc
     4021 tgcagcttca tgttgctcac cacgccggcg gcgggcgcct tgatctgggt gcgggcgagg
     4081 tcgatggccg cggcatcgta ggccgtcttc gcctcgacgt agcgcgggtg ccgctccgcc
     4141 gggagctgcg gatcgccggc aaggctcgcc cgcacccggt tgatgcgctc ctcgacgctg
     4201 gccagtcgcg cacgggcgac ttccagatgg tggcgcgcct cgtcgtaggc ctcgatacgg
     4261 ctcatgccct tctctttcaa gcgctcctgc cgctcgagct gccgcgtcag gaaatcgatg
     4321 cgctcgcgcg cctcggtgga ctccagcagc gtttcccgga attcggcgcg caacgcctgc
     4381 acctcggtgc gcaccacgtc catctgggct cgcgcccgcg ccagctcgat ctcgaacggc
     4441 ttggcatcga gacggaacag cggagcgccc gcggccaccg gctcgttgtc gcgcgcggcg
     4501 acctcgacca cgcgcccgga cacctcggcg ctgacggcga tgatgtcggc cttgacgtag
     4561 gcgttttcgg tttcctcgta gcggccgccg tgcgcgtaca cgtacagccc gacgaggatc
     4621 gcagcgaacg gcacgccgaa cagcaacaac cagcgcgtga cccggcgcac cgcgatgacc
     4681 gcgtttccca ccacgggcgc catgctcatc gcttgcgccc ccgagcgccg ttgcgggcca
     4741 cgccgttgtc ggatccgttc agattggcct tgacggcaag cagcgtatcg acgaagcgct
     4801 gctgctcctc ggcggaaagc ccggccaacg cgtcccggcg catctcggcg gccgccgcgc
     4861 gcatcgcccg cagcgcgggc tcgatctcct cggtgaggaa cacgcgcttg acgcgccggt
     4921 cggcggcgtc ggcctcgcgc cgcacccagc ctttcgcctc cagacggtcg agcaggcggc
     4981 ccagggtggg cttctccacc tccagaatgt cggcgagctc ggactgcgtg atgccgtcgt
     5041 tgcggaacag gtgcgtcagc acccaccatt gcgagcgcgt gaagccgagc gccttgacac
     5101 gacggtcgaa ggtggtgcgc agcaggcgcg cgacgtcgtg caggatgaag ccgaagttcc
     5161 gggacaggtc ttcgcggaac atgtttccag gagggggctg ttaccggcca aagtttagtt
     5221 gtctagctta taataagcaa gcttcatgcg cgttcgccag cgctcgcccc gggggcgcgg
     5281 ccggggagtc gtcgcctgcg gtcatccgct agaatccacc ggtccgttcc gccaaccgcc
     5341 gccagacgag aacatgaaag tcaccgccat cgaaaccatc aacctggacg agtttcccaa
     5401 cgtgctgtgg gtgcaggtgc acaccgacga gggcatcgtc gggctgggag agacgttcta
     5461 tgcggtggag ccggtcatcg cccacatcca ccagaccatc gcgccgtatc tgctcggcaa
     5521 gagcccgctg acgatcgacg cccacagtcg ccatgtgctc tacaactacc tcggcttcaa
     5581 gagcgtcggg gcggagatgc gggcggcctc ggcggtggac atcgcgctgt gggacatcct
     5641 cgggcaggtg agcaaccagc cgatctacca gcttctcggc ggcgccagcc gggacaaggt
     5701 gcgcgcctac aacacttgcg ccggctacca gtacgtgcgc gccaagtccg agcaaggcac
     5761 cgccaacttc ggcatgccgg gcgacagcgg caagtaccag ccgtacgagg atctggtcgg
     5821 attcatgcag tatgcggacg agctcgccct gtcgctgctc gaagaaggct ataccggcat
     5881 gaagatctgg ccgttcgacg agtatgccga ggcgagcaac ggcacctaca tctccagcgc
     5941 cgacctgaag aaggcgatgc ggcccttcga gaagatccgc aaggcggccg gggacaggat
     6001 ggacatccac gtggaattcc attccctgtg gaacctgccc accgcgatca agatcgccaa
     6061 ggcgctggag cagttcgacc cgttctggta cgaggacccg atcaagatgg acaacctcga
     6121 cgccatcgcc gagtacgccc gtcgcaccga cgtgtgggtc accgccagcg agacgctcgg
     6181 cacgcgctgg gcgttccgcg atctgttcga gaagcgcgcc gtgtcggtgt gcatgttcga
     6241 cgtcggctgg accggcgggg tgagcgagtc gaagaagatc gccaccatgg ccgaggccta
     6301 tcagctgccg gtggccccgc acgactgcac cggcccggtg ctgctgaccg cgtcggtgca
     6361 cttgtcgatg aactgcccca acaccctggt gcaggaagtg gtgcgcgcct tctacagcga
     6421 ctggtacggc aagctggtca cgcagctgcc gccgctggag aagggctata tcagcgcgcc
     6481 gaacggtccg ggcctgggta cgcgcctgca gcccgacgtg ctcaagcgca aggacgcacg
     6541 catccggcgc agcagtctgg cgtaagcgct cgccgcccca tgccccgcat cccctaccaa
     6601 cccgaagatc tgcgcgagcc cgccgcggtg gtcgaggcga tccgccagcg ccgcggcggc
     6661 cgcctgtacc atctcgatcg gctgctgctg cacagcccgc cgttcgccgc cgcgtggaac
     6721 ggcttcatgc gcgaggtcag gacgctgtcg attccgccca ggctgcgcga aatcgcgatc
     6781 tgcgcggttg cgtgcctcaa ccgcgccgaa tacgagttcc accatcacgc gccgctgttg
     6841 ctcgcggccg ggggcaccgc cgagcaggtg gaggccttgc gccggctgga gcagggcggc
     6901 gcgcaggcac cggtcttcga ctctgcggag cgggccgtga tccggctcac caccgagatg
     6961 acgcgcgcgg tgcaggtcgg cgacgatacc ttcgcggcgg ttcgtgccgt gctgccgagc
     7021 gagcaacagg cggtggagct ggtcggcgtg atcgccgcct acaacatggt gtcacgcttc
     7081 ctggtcgcgc tcggtgtcga gccggaatag ccgcctcggc gtcgcaatcg gccgctcgga
     7141 ccgcggcgtc gcccgacagc gacgagcgcg cctgacaagc aggtcaaccg agccgcgcag
     7201 caggtgaaag acgagaagac gcagcgcgct atcaccaaag tgatagagac tgctccgcat
     7261 gcgggattgt ggtccggtat gccatctgta atgatccgag cccagaagac gttctcttct
     7321 cggtcatggg gtgcagttgc ctacgaccgg tttgcttgac ccgcccccca ggcggaaact
     7381 caacgatcag gaggaaacac atgcgtgctc tgatttctgc tctcgcggcg gctggcctgc
     7441 tctcgctcgc gctgccgacg ttcgcgcaag acacggtcaa gtcgattccg gacgccaaca
     7501 agccgggacg gcaggctgag gacgacacca agaaggcggt gaagtccaag cccgacctca
     7561 gcaagcccgg ccgcaccgcc gaggaagaca aggccgtcaa gtccgccggc ccgtccaaca
     7621 agcccggtcg caccgccgag gaagacaagg cgatcaaggc cggtggcgcg tccaacaagc
     7681 ccggccgcac cgccgaggaa gacaaggccg tcaagtccgc cggcccgtcg accaagcccg
     7741 gccgtaccgc cgaggaagac aaggccgtca aggccggtgg cgcgtccgac aagcccggcc
     7801 gcaccgcgga agaggaagcc aaggccgtca agtccgctgg cccgtccgac aagcccggcc
     7861 gcaccgcgga agagaagaag taatgccgca ccgctgaacc cggttcagcg cacgtaaaaa
     7921 gggcggggac tccccgccct tttccttttg gcgctcccgg tcacccccac tacgtcattt
     7981 gcgcagcaac cccaggcgcc gcgcttcgtt caccgcctgt gtgcgattgc gcgcgttcaa
     8041 gcgcttgaac agatccttca agtgctgctt gacggtgcct tcggtaacgc ccagcgcccc
     8101 ggcaatctgc ttgttggcca tgccgtcggc cagcagcggc aggatctgca gttgccgatc
     8161 ggtgagggtc gcctcgccgt tgcctcccgg cgcctgcgac gcgggcagga acaacccctc
     8221 tgggacgaag acctggccgc gcaggacggt gcgcagcccc tccatcagca gctcgctgct
     8281 ggcgaacttc gggatgtagc cacgcgcacc gccgtcgagc agggtgcgga tgatgcgcgt
     8341 gtcgctcacg cccgaaacga tcaccacggg caaggcggga tagcgccggc ggatggcgaa
     8401 gaagccgtcg agctcgccgc tgccgggcat ctgcagatcg agcagcagca ggtcgaccgg
     8461 cccctccagc gccaacttgt ccagcgccgc ctggaagttg ctcgcttcca ggatggtcac
     8521 ctggggatcg agaagctgga ggaagaacct cagcccctcg cgaaacatcg ggtggtcgtc
     8581 ggcgtgcagg atcttcatgt cgacggcgcc gcgcgcggcg accggcgcgg gcttgggctc
     8641 gccgtcatcc tggggctcac cgtcatcctg cggctgcgcg gttgctcacc tcgcgccgcg
     8701 cgccgcgccc agcggccgcg gacggcagcg tgaagctgaa ctcgttgccg ccgccggggg
     8761 ccggctgcac ggcgatgctc ccgccgagcc tggagatgat gcgctccacg gtggccagac
     8821 cgagcccgtg cccttccgcg cgctcctgat gcaagcgcgt gaacggggtg aagacgcggc
     8881 ggcgctgctc ctcggacagc ggctggccgt tatcgcgcac ccagaagcgc gcctgcgtgc
     8941 cctcgagtgt cgcgcccagg tccaggcgcg gcggcgagcc gctgtacttg agcgcgttgc
     9001 tcagcagatt catccacacc tcgccgaccc acggcgcata gccgagcgcc gccggccagc
     9061 tcttcgggag ctgcacggcg gcgcgtgtgc tggcctgcag cccggcgagc tggttcaagg
     9121 cctggcccac cagcgcactc atgtccagcg gatgcagctc cacacgctcc tgcgacaccg
     9181 tggacagcag caacagggcc tcgaccgcgc tcgcgctggc ggccagtgcc gcctgcgcct
     9241 gctgcagcag gtggcgttgc tcttcggaca ggttggcgct cttgaacttg tgcaggatct
     9301 ccaggaagcg ggcggcggcg gccagcggcg tcttcaggct gtgcgcgatg gtatgcgcat
     9361 aggcttccag ctcggcgttc ttcagcgcca ggtcctgagt cttggagtac agctcctgtt
     9421 gcagccggtg cagctgcagg tgatgctgga tgcgcgccag cagctcttcg cgctggatcg
     9481 gtttggtgat gtagtccacc gcgcccaggg cgaagccctc gaccttgtgc gcgatgtcgt
     9541 ccaccgcggt catgaagatc accggcagcg cggcggtcgc gggatcggct ttcagtcgcc
     9601 ggcagacctc gaagccgtcc atgtcgggca tgcgcaggtc cagcagcacc agctcgggag
     9661 cggcgagccg cgcccgctcc agcgcctgcg cgccgttgcg ggtgagcatc agctcgaagc
     9721 cctgctcggc caggaaatcg gagatcagcc gcaggttctg cggcttgtcg tccacgatca
     9781 ggagcgtggc accgcgcagc tcagtcgcta acggcatcgt ggtgcgcttc gagggcttcc
     9841 tgcagcaggc cgacgaggcg ctcttcgtcg aagcgccgcg ccagcccgta cagccggcgc
     9901 gcgaagggac cgtagcgcgg gtcgccctgc tccagctcca gggcgatctg ctccacccgc
     9961 accagcttgc ccagcctggc caggtcgagc agattctcga gcacgggtgc gggcggagcg
    10021 accatccgcc cctcgcgccc gtcctccggc tccgcgctct cggcctcgcc gtagcgccag
    10081 ctcaacccga gcacgcgctc gatggcgtcc agcagatcgg cggtttgaaa cggcttgcgc
    10141 aagcacacgg cgaaggtagc gggatcggct tcggcgcgcg ccaggtcggc ggtgctcgcc
    10201 gaggcggcca ccagcggcac gcgctccagt cccggcaggc
//
LOCUS       DASPGE010000157         5645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_746808, whole genome shotgun sequence.
ACCESSION   DASPGE010000157 DASPGE010000000
VERSION     DASPGE010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746808"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2045
                     /locus_tag="VD791_04975"
     CDS             <1..2045
                     /locus_tag="VD791_04975"
                     /inference="COORDINATES: protein motif:HMM:NF009589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXV07358.1"
                     /translation="MGLAPFTDHPHMFVNLGDGTYFHSGLLAIRQAVAANLSITYKVL
                     YNDAVAMTGGQPVDGSLSVPQMIAQLRAEGVARIALLSDHPERYAQAPLTDREVPVLD
                     RSQLDRVQRELREHKGVSVLIYEQTCATEKQRRHKRANIEAPLLQPVINDEVCEGCGD
                     CGVASNCTAIVPLETALGRKRAIDPTHCTQDLSCLDGFCPSFVLVEGGRRRGAPVRDL
                     EREAQARMSALPEPRLPDLARPCNVLVAGIGGTGILTTSGILGVAAQLDGIGALVLDM
                     TGMSQKNGGVTSHVHFAARQAELSAARVAVADADLVLAVDLLVACSEQGLATMVPGRT
                     RFVGNLALVMPGQFTKQPDLDFPLERMRATVERRLGASAAHWIDANRLAERLVGDLMP
                     VNLFLLGYAWQKGLLPLTRQAIERAVALNGVAVEKNLNAFLWGRRAAHDQAAVERIAV
                     PPQPVRLQRRTALAELIADRKARLTAYQDAAYAERYLQLVERAGAAERSRSPGRQGLA
                     EAVARYYFKLLAYKDEYEVARLYVDSGFLERVASQFEGDYKLSFNLAPPLFAPEDPST
                     GLPRKRRFGPWLVTAFRLLAKLKGLRGTRFDPFGWAAERRMERRLIAEYEALIEQILA
                     ELTRDNHAVAVALASLPDQIRGFGHVKGRNVEAAARERAKLLEEFRRAPHPLATAA"
     gene            2201..2629
                     /locus_tag="VD791_04980"
     CDS             2201..2629
                     /locus_tag="VD791_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009921893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXV07359.1"
                     /translation="MPAQPMWNVFDDRCPTRLVLNRVADKWTVLIVARLARRTMRFGE
                     LKRDIAGISQKMLTQTLRALERDGLVARAVYPEVPLRVEYSLTPLGATLVSILVQIKD
                     WAEGNIEAVLAAQRAYDQAAGQPVQPQGPARVVRLASRAA"
     gene            2647..3444
                     /locus_tag="VD791_04985"
     CDS             2647..3444
                     /locus_tag="VD791_04985"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXV07360.1"
                     /translation="MGRLDGCVALITGSGRGIGRAMALRYAREGAKVAVVDLDESAAQ
                     SVAREIRALGATADAFKSDVREPGESAAMVARVAQRFGRLDVLVNNAGVMRVRPLLEL
                     TPEDWDFVNSVNARGLFFALQAGARQMMRQAPPGPGRPRGKIINVASIAGRIGRPMFA
                     AYAASKAAAISITQSGATALAPDITVNAICPGVVETEMWRQIDREWTEIERRPPGSAW
                     EDRIRGIPMGRPEAPEDLAGMAVFLASADSDYITGQSYHVDGGLVMS"
     gene            complement(3454..>5645)
                     /locus_tag="VD791_04990"
     CDS             complement(3454..>5645)
                     /locus_tag="VD791_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07361.1"
                     /translation="AVAAGLYALSFALPTAASGASCTPQTFLERCALADYLQRLSGAA
                     APAPVLGAIVVLTALAGVLSWLVDINLFSFHSFYRNRLVRCFLGASNGARRALPFIGF
                     DAADNPRFADLVQRPYHIVNTALNLTRSKRLSWQERKAGSFVLTPLYCGYQFPDLSAR
                     ERPEASGEPCFQPTAEYAKDENGYLSLGTALAISGAAASPNMGYHSTPSVAFLLTVFN
                     VRLGWWIQNPSSAANWRQGGPYLGLRYLLSELLGLTSEQTPFVYVSDGGHFENLGIYE
                     LVRRKCRFIVASDAGQDEAFEFEDLGNAIRKCQIDLGVRIEIDRRAIIPDPATRRSLY
                     HCAVGTVRYRDGAPGYLLYIKPTLTGNEPADVSQYAAAHGDFPHQPTGDQWFSESQFE
                     SYRKLGQHIGWTALRDAGLEPGMDRESIFVALKERWYPPSGAVADAFTRHAEALAALQ
                     QIQRGDGLLRFLDMQVYPEWERLDQVAPGGARNLWLPHAPEELRAGFYFCRSMLELME
                     NVYLDLDLEEEYDHPDNRGWMNLFRHWSWSGMFKVTYAICCSMLGARFQRFCERRLDL
                     WPGDVAVVSEAVDAAQLGAFLTRAEAQKQLNFVEAEMIERLHRGGAAFDRIELFQLEV
                     WSPETLDRANAEPGSSFVYTFGFALTRGQRLVYFRIQDHLRKMGHARRALSRLCYRKQ
                     APVTGHAVDELRQLGIDWLEPQDFDGFERLFKSVQQQGRDPDAARRP"
BASE COUNT          888 a         1946 c         1988 g          823 t
ORIGIN      
        1 ggatgggtct ggcgccgttc accgaccacc cgcacatgtt cgtgaacctg ggcgacggca
       61 cctatttcca ctcgggcctg ctcgccatcc gccaggcggt cgcggccaac ttgagcatca
      121 cctataaggt gctgtacaac gacgcggtgg cgatgaccgg cggtcagccg gtggacggct
      181 cgctgtcggt gccgcagatg atcgcccagc tgcgcgccga aggcgtcgcc cgcatcgcgc
      241 tgctgagcga ccatccggag cgctacgcac aggcgccact caccgatcgc gaggtgccgg
      301 tgctcgaccg cagccagctc gatcgcgtgc agcgcgagct gcgcgagcac aagggcgtgt
      361 cggtgctgat ctacgagcag acctgcgcca ccgagaagca gcgccggcac aagcgcgcca
      421 acatcgaggc gccgctgctg cagccggtga tcaacgatga ggtgtgcgaa ggctgcggcg
      481 attgcggcgt ggcctccaac tgcaccgcga tcgtgccgct ggagaccgcc ctgggacgca
      541 agcgcgccat cgacccgacc cactgcacgc aggacctgtc ctgcctggac ggcttctgcc
      601 cgagcttcgt gctggtggag ggtggccggc gccgcggcgc tccggtgcgc gatctcgagc
      661 gcgaggcgca ggcgcgcatg agcgcgctgc ccgaacccag gctgcccgat ctggcgcggc
      721 cctgcaacgt gctggtcgcc ggcatcggcg gtaccggcat cctcaccacc agcggcatcc
      781 tgggcgtggc ggcgcagctc gacggcatcg gcgccctggt gctggacatg accggcatgt
      841 cgcagaagaa cggcggcgtg acctcgcacg tgcacttcgc ggcgcgccag gcagagttgt
      901 ccgctgcgcg cgtggcggtg gccgacgccg atctggtgct cgccgtggat ctgctggtgg
      961 cctgcagcga gcaggggctg gcgaccatgg tgcccggacg cacacgcttc gtcggcaacc
     1021 tcgcgctggt gatgcccggc cagttcacca agcagcccga cctcgacttc ccgctggagc
     1081 gcatgcgcgc caccgtggag cggcgcctgg gcgcctccgc cgcgcactgg atcgacgcca
     1141 accggctcgc cgagcgtctg gtcggcgacc tgatgccggt gaatctgttc ctgctcggct
     1201 acgcctggca gaaggggctg ctgccgctga cccgccaggc gatcgagcgc gccgtggcgc
     1261 tgaacggcgt ggcggtggag aagaatctca acgcattcct gtgggggcga cgcgcggcgc
     1321 acgatcaggc ggccgtggag cgcatcgccg tgccgccgca gccggtgcgc ttgcagcgca
     1381 ggaccgcact ggccgagctg atcgccgatc gcaaggcgcg actcaccgcc tatcaggacg
     1441 cggcctacgc cgaacgctac ctgcagctgg tggagcgcgc gggcgccgcg gagcgcagcc
     1501 gcagcccggg caggcagggg ctggccgaag cggtggcgcg ctactatttc aagctgctcg
     1561 cctacaagga cgagtacgag gtggccaggc tgtacgtgga cagcggcttt ctcgagcgcg
     1621 tggccagtca gttcgaaggc gattacaagc tcagcttcaa tctcgcgccg ccgttgttcg
     1681 cgcccgagga tccgtccact ggattgccgc gcaaacgccg cttcggcccg tggctggtca
     1741 ccgctttccg gctgctggcg aagctcaagg gcttgcgcgg cacgcgcttc gatccgttcg
     1801 gctgggccgc tgagcgcagg atggagcgcc gcctgatcgc cgagtacgag gcgctgatcg
     1861 agcagatcct cgccgagctc acgcgcgaca accacgccgt ggccgtcgca ctggcctcgc
     1921 tgccggacca gatccgcggc ttcggccacg tcaaggggcg caacgtcgag gccgccgcgc
     1981 gcgagcgcgc gaaactgctg gaggaattcc gccgcgcgcc gcatccgctc gcgacggcgg
     2041 cgtgacctgg tgcgccctta aggcgcgatt tgaaaacctg ttcccgggct cgcagcccac
     2101 tctgccatcg cccgccagca ccagcggttg cctgctgcag aaagcgcgct taccatgcgc
     2161 aagtaagcac cggccggtaa ccggtcacct cggagcgacc atgcctgcgc aacccatgtg
     2221 gaacgtgttc gacgatcgct gccccacgcg tctcgtgctg aaccgcgtgg cggacaagtg
     2281 gacggtgttg atcgtcgccc ggctcgcgcg ccgcaccatg cgtttcggcg agctaaagcg
     2341 cgacatcgcc ggcatctccc agaagatgct cacgcagacc ctgcgcgccc tggagcgcga
     2401 cggcctggtc gcgcgggcgg tgtatccgga ggtgccgttg cgcgtggagt actccctcac
     2461 gccgctcggc gcgaccctgg tgagcatcct ggtgcagatc aaggactggg ccgagggcaa
     2521 catcgaggcg gtgctcgccg cgcagcgcgc ctacgaccag gccgccgggc agccggtgca
     2581 gccgcaaggg ccggccaggg tggtgcgcct ggcctcgcgc gcggcctgag ccgggacgca
     2641 acccacatgg gcaggctcga cggctgtgtg gcgctgatca ccgggagcgg tcgcgggatc
     2701 gggcgcgcca tggcgctgcg ctacgcgcgc gaaggcgcca aggtggcggt ggtcgatctc
     2761 gacgagtctg ccgcacagtc cgtcgcccga gagatccgcg cgctgggcgc cacggcggac
     2821 gccttcaagt cggacgtgcg cgagcccggc gagagcgcag cgatggtcgc gcgggtggcc
     2881 cagcgtttcg gccggctcga cgtcctggtc aacaacgccg gcgtgatgcg cgtgcggccg
     2941 ctgctggagc tcacccccga ggactgggat ttcgtcaaca gcgtcaacgc gcgcggtctg
     3001 ttcttcgcgc tgcaggcggg cgcgcgccag atgatgcggc aggcgcctcc gggcccggga
     3061 cggccgcgcg gcaagatcat caacgtcgcc tccatcgccg gacgcatcgg ccggccgatg
     3121 ttcgcggcct acgcggccag caaggccgcc gcgatcagca tcacccagtc gggggccaca
     3181 gcgctcgccc cggacatcac cgtcaatgcg atctgtccgg gtgtggtcga aaccgagatg
     3241 tggcgccaga tcgaccgcga gtggaccgag atcgagcgac gcccgccggg ctcggcctgg
     3301 gaggaccgca ttcgcggcat cccgatgggc cgcccggagg cgcccgagga cctcgccggc
     3361 atggcggtgt tcctggccag cgcggactcc gattacatca ccggccagtc ctaccacgtg
     3421 gacggcgggc tggtgatgag ctgagaggtt caatcacggc cggcgcgcgg catccggatc
     3481 ccggccctgc tgctgcaccg acttgaacag gcgctcgaag ccgtcgaagt cctgcggctc
     3541 gagccagtcg atccccagct gccgcagctc atccaccgcg tggccggtga ccggggcctg
     3601 cttccggtag cacagccggc tgagcgcgcg ccgcgcgtgc cccatcttgc gcaggtggtc
     3661 ctggatccgg aagtacacca gccgttgccc ccgcgtcagc gcgaagccga aggtgtacac
     3721 gaagctcgag ccgggttcgg cgttggcgcg atccagcgtc tcggggctcc acacctcgag
     3781 ctggaacagc tcgattcggt cgaaggcggc gccgccccgg tgcagccgct cgatcatctc
     3841 ggcctcgacg aagttgagct gcttctgcgc ctcggcgcgc gtcaggaacg cgccgagctg
     3901 cgccgcgtcc accgcttcgg acacgaccgc gacatcgccc ggccacaggt ccagccggcg
     3961 ctcgcagaag cgctgaaagc gcgcaccgag catcgagcag cagatcgcat aagtgacctt
     4021 gaacatgccc gaccacgacc agtgccggaa caggttcatc cagccgcggt tgtccgggtg
     4081 gtcgtattcc tcctcgagat cgagatccag gtacacgttc tccatcagct ccagcatgct
     4141 gcggcagaag tagaagccgg cgcgcagctc ctcgggcgcg tgcggcagcc acaggttgcg
     4201 cgcgccgccc ggcgccacct gatcgaggcg ttcccactcg gggtagacct gcatgtccag
     4261 gaagcgcagc aggccgtcgc cgcgctggat ctgctgcagc gccgccagcg cctcggcgtg
     4321 gcgcgtgaac gcgtccgcga cagcgccgct gggcggatac cagcgctcct tgagcgcgac
     4381 gaagatcgac tcgcggtcca tcccgggctc gaggcccgca tcgcgcagcg cggtccagcc
     4441 gatgtgctgg ccgagcttgc ggtagctttc gaactgcgat tcggagaacc actggtcgcc
     4501 ggtaggctgg tgcgggaagt cgccgtgcgc ggcggcgtac tgcgagacgt ccgcgggctc
     4561 gtttccggtc aaggtcggct tgatgtacag cagatagccc ggggccccgt cgcgatagcg
     4621 cacggtgccg actgcgcagt gatacagcga acgccgcgtg gccggatcgg ggatgatggc
     4681 gcgcctgtcg atctcgatgc gcacgcccag gtcgatctgg cacttgcgga tcgcgttgcc
     4741 gaggtcctcg aactcgaaag cctcgtcctg gccggcgtcg ctcgccacga tgaagcggca
     4801 cttgcggcgc accagctcgt agatgccgag gttctcgaag tgcccgccgt cgctcacgta
     4861 cacgaacggc gtctgctcgc tggtgaggcc gagtagctcc gacagcaggt agcgcaagcc
     4921 gagatagggg ccgccctgcc gccagttggc cgcggagctc ggattctgga tccaccaccc
     4981 caggcgcacg ttgaacaccg tgagcaggaa cgcgacgctc ggggtcgagt ggtagcccat
     5041 gttcgggctg gcggcggcgc cggagatggc gagcgcggtg ccgagcgaga gatacccgtt
     5101 ctcatccttg gcatactccg ccgtcggctg gaagcagggt tcgcccgagg cctcgggacg
     5161 ttcccgcgcg ctcagatccg ggaactgata gccgcaatac aacggcgtga gcacgaagct
     5221 gccggccttg cgctcctgcc agctcagccg cttcgagcgc gtcaagttga gcgccgtgtt
     5281 gacgatgtgg tagggacgct gcacaagatc ggcgaagcgc gggttgtcgg cggcatcgaa
     5341 gccgatgaac ggcagcgccc ggcgcgcgcc gttgctcgca ccgaggaagc aacgcaccaa
     5401 gcggttgcgg taaaagctgt ggaacgagaa caggttgatg tccaccaacc acgacaggac
     5461 tccggccagc gctgtcagaa cgacgatcgc gccgagcacc ggcgcgggcg cggcagcacc
     5521 gctcaggcgc tgaagatagt ccgccagcgc gcagcgttcg aggaaggttt gcggggtgca
     5581 cgaggcgccg ctcgcggccg tggggagcgc gaacgacagt gcgtagagcc cggccgcgac
     5641 ggcca
//
LOCUS       DASPGE010000158         9638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_980798, whole genome shotgun sequence.
ACCESSION   DASPGE010000158 DASPGE010000000
VERSION     DASPGE010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9638
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980798"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VD791_04995"
     CDS             <1..1002
                     /locus_tag="VD791_04995"
                     /inference="COORDINATES: protein motif:HMM:NF044995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07362.1"
                     /translation="PSAEDPQSHGIVHVSNGVRTPLDPAAIQGGQLGGLLALRSDALD
                     PVQNQLGRVAVALAQTFNDQHRLGMDLQGDAGQDFFRVPAAVVLENADNTGTAVIAAD
                     SGNVGALTGSDYRLQHSAGTWTLTRLSDNVGTVFSGFPHTVDGFTLTLASGAAASGDS
                     FLIRPVRDGARDIAVLISDAARIAAAAPARYDAFGIFDTRSGGIYDTRSGGIVDARSG
                     GIVDNRSGGIYDNRNALLLAALQGHGTIAGGSASYQSACDRIVQEVRDRAHAVEVQSK
                     AQDALVRQAREAQRSLPGANLDQEAATLFRHQQAYQACGKALQAAARLFDTLLETSE"
     gene            1070..1492
                     /locus_tag="VD791_05000"
     CDS             1070..1492
                     /locus_tag="VD791_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07363.1"
                     /translation="MLVKGRPVGILLLGLWCAAHTVPFGLLAADASGGKAFLAWVAVI
                     VALLAAAGLLIRSRIAHWVVVLQVGVHVFVFSVAAWAFLFVALAWGLHASETAVVALI
                     FAYLSFTCWALLYLFYPDVLDYFEESRSFFRPASHDGL"
     gene            1615..2445
                     /locus_tag="VD791_05005"
     CDS             1615..2445
                     /locus_tag="VD791_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011004196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HXV07364.1"
                     /translation="MVLAVVFPLPGGAACPDDAAVAAYVADYRAARMSRGFGNDLSLA
                     EAQCAKAKLTRQLPQVQGAVVGYKAAFTNPAMYERFGVTGPAWGVMFAKMMVDSGAKL
                     PARFGARPLYEADFLAVVKDAGLADARTQLEALQHISAVVPFIELPDLMLEGPATGAA
                     LIGTNLGFRGGALGPRVAVEPTQAFLDALANMTVVMTEDKSGKELGRAKGDILMQHPI
                     NAAMWLAQALKKDGVALKPGDLLSLGGYIPPAPTQSGTTITVKYLGLPGNPAVTVHLE
                     "
     gene            complement(2532..3326)
                     /locus_tag="VD791_05010"
     CDS             complement(2532..3326)
                     /locus_tag="VD791_05010"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXV07365.1"
                     /translation="MTTGFLWAFVLAAFVGALALGLKQSRRIRQLERLVQDTHGKLER
                     LQLHFARFAPDDVIERLTEPGGELKPTRRTVTVLFADLKGFTALSDRMDPEQMVPILN
                     GYFRAMSAAITAHHGRVTELMGDGMLALFGALEPNPWQAQDAVFGALAMREALARYND
                     TLRARALPELQVGIGIHRGEVLAGVMGNEELSKFGVVGDTINVASRVEQLTRELSVDL
                     LITEEVRRELDGRFRLRPMPPQAVKGKPDPLVTYLVEGLESAVQPA"
     gene            complement(3323..4396)
                     /locus_tag="VD791_05015"
     CDS             complement(3323..4396)
                     /locus_tag="VD791_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07366.1"
                     /translation="MAQLLSDIEWGPPLVPPVVVAEWETEAKQRFGGSSDFLRRVAPV
                     PWLRRAVATWTQYPIKALPVRMADLVFLVVAQENSCRYCYGAARAHMRILGYSERVIG
                     QIERQMQLAELDEKDQAFIRFCRSLARSNPRPAREQREELIRVGFSPQQVTEAAFLIV
                     NNCFGNRVATFIALPPATGYERIGRSPFGRLLRPLLARANRVSVPIPADFVPREHEPF
                     AGVIAPLVGHPAAYLLNETLRGAFESPILSRRVKALMFAVVARILECRFCEPESRKLL
                     HAEGLDDAEVEASLSALALPKLDAREAAVLAWVRDTVRYQPSQIQARTRALAEAIGGE
                     AALEAVGVAALANSTVRVAMLLE"
     gene            4564..5919
                     /locus_tag="VD791_05020"
     CDS             4564..5919
                     /locus_tag="VD791_05020"
                     /inference="COORDINATES: protein motif:HMM:NF042664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HXV07367.1"
                     /translation="MANGAGTCFVVMGFGKKTDFESGRTFDLDKSYRNLIKPAVEAAG
                     YRCVRADEIVHSGLIDVPMYEQLLAADIVVADLSTANKNAFYELGIRHALRPYTTIVI
                     AEDGLKPPFDVNHVVVRHYKHLGEDIGFDEVMRFRKLLTEAITVIAAQPVDKRVDSPV
                     YRFVAGLNPPALAAVAAAPPAPAAVRAPAAAGGATHSALMQQVDDAVARKDFVAAKSL
                     LTVVHDMMRAEAPDRGEDPYIVQRLALLTYKSKHPSEEASLREALALLAGFDPDTSND
                     TETLGLWGAVHKRLWSLTGERPHLDASVRAYARGFYLRNDYYNGINLAFLLDLRASRA
                     EPADAIADHVLARRVRKEVRQICEKWLAQNPPAAAPADAGPQAARQAAQQAAEHRYWV
                     LATLGEAMLGLGDPAAQQQLEAAYAAAPYAWMADTTREQIAKLEPLLADSPLKYLKPA
                     A"
     gene            6098..7114
                     /locus_tag="VD791_05025"
     CDS             6098..7114
                     /locus_tag="VD791_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV07368.1"
                     /translation="MTRVTAANLIGRSAAFQETVRLVQRIAQTDAPVLIEGETGTGKE
                     VAARAIHYSGVRRDRPFVPVNCGAIPENLIENELFGHVGGAYTDARGPQFGLIALANG
                     GTLFLDEVETLCPKGQVALLRFLQDLNYRPLGSRHEQSADVRVIAATNINLSELVQSK
                     QFRMDLYYRLQILFLRIPPLRERDGDPALLAEHFARAFSARYGGPATRFAADSLALLG
                     GYAWPGNVRELENLVHRAFLLAGGDVISIPHDWLTNEPRPAAADAHQVFPLDLAFSEA
                     KARLIDQFERVYIAHVLSQTNGNVSQAARRCGKERRAFGKLLKKHGIDKRLFERPTRV
                     AAHP"
     gene            7327..7575
                     /locus_tag="VD791_05030"
     CDS             7327..7575
                     /locus_tag="VD791_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07369.1"
                     /translation="MPDLAPEVRALVSRIRQYILTRSQAADSVKGVAGAWLELSGEEA
                     WLPVVEQALEHLVATGEMQRQRLADGTVMYSVATRIRH"
     gene            7712..9079
                     /locus_tag="VD791_05035"
     CDS             7712..9079
                     /locus_tag="VD791_05035"
                     /inference="COORDINATES: protein motif:HMM:NF015380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HXV07370.1"
                     /translation="MAKATGRKAAKKKGSGKKATEKKATKSPKKASKTMGKAAWTVMV
                     YLAGDNDLDSAGVVDLGEMKEVGSSDRINLLAQFDRAGPRIETRRFLLRKGTPLGRDL
                     VKSLGETNMGDPKILKDFLVWGAKNYPAERYMVVIWNHGAGWDDENVYRAVRGGLKRG
                     VAYKKHAVGRPARGAKRAVPLSRLRSVSKRKFKRALFSSTVQQAVEKRAIAFDDDAQD
                     FLDNIELKRVLTGARKVFGGKIDVLGMDACLMNMAEVAYQVRNTAQVLVGSQEVEPAD
                     GWPYHTILRDLAKKPGMTPAELGKVIVGRYLASYGANEGVTQSALDLSLCRGLEAAID
                     KLAAVLIGALPGDDARLGLLQARQAVRDYDTRDYIDLVHFCQLLRKFSGSAAIHAACD
                     AVDGAAVKLVLASGFKGTGVKNSNGVSIYFPEGSISPLYAKLDFARNNKWNEMLRAYF
                     DAVSA"
     gene            complement(9091..>9638)
                     /locus_tag="VD791_05040"
     CDS             complement(9091..>9638)
                     /locus_tag="VD791_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07371.1"
                     /translation="EEADFPGATIHAACGLGPVYNLMQRPRDAIPLLERAWGLAEAGG
                     FVYFGVVCLMHVADAYSLTGQDARAWDCAEHALKIADDADFPARKAWALYVRGRILAR
                     ASDSDAAAAREAYQAALGGAGQLGLQPLVAHCHLGLGQIEARARERGKAREHLTTALS
                     LYRAMQMQYWPGQVESALGAH"
BASE COUNT         1556 a         3316 c         3296 g         1470 t
ORIGIN      
        1 ccctcggccg aggatccaca gagccacggc atcgtccacg tgtcgaacgg cgttcgcacc
       61 ccgttggacc ccgccgcgat tcagggcggg cagctcggcg ggctcctggc gctgcgcagc
      121 gacgcgctcg atccggtgca gaaccagctg ggacgtgtcg cggtggcgct ggcgcagacc
      181 ttcaacgacc agcaccggtt gggcatggat ctgcagggcg atgcggggca ggacttcttc
      241 cgcgtcccgg ccgcggtcgt gctggaaaac gcggacaaca ccggcacggc ggtgatcgcc
      301 gccgacagcg gcaacgtcgg tgcgctcacc ggcagcgact atcgcctgca gcacagtgcc
      361 ggcacctgga cgctcactcg gctcagcgac aacgttggca ccgtgttctc cgggtttccc
      421 cacacggtgg acggattcac gctcacgctc gcgagcggcg cggcggccag cggcgacagc
      481 ttcctgattc ggcctgtacg cgacggcgca cgcgatatcg cggtgctgat ctccgacgcg
      541 gcgcggatcg ccgccgcggc gccggcgcgt tacgatgcct tcggtatctt tgacacccgc
      601 agcggcggca tctatgacac ccgcagcggc ggcatcgtcg acgctcgcag cggtggcatc
      661 gtggacaacc gcagcggcgg catctacgac aatcgcaacg cgctgctgct ggccgcgctg
      721 cagggacacg gaacgatcgc cggcggcagc gccagctacc agagcgcgtg cgaccggatc
      781 gtgcaagagg tccgcgaccg ggcgcatgcg gtcgaagtgc agagcaaagc ccaggacgcg
      841 ctggtcaggc aggcgcgcga ggcccagcgc tcgctgccgg gcgcgaacct ggaccaggag
      901 gcggcgacgc tgttccgtca ccagcaggcc tatcaggcct gcggcaaggc gctgcaagcc
      961 gccgcccgcc tgttcgacac tctgctcgag accagcgaat gatccgcttc gattccggcc
     1021 ccggctccgg ccgcagcgca tggcattcgc ccggtgcagg ggagagcgca tgctggtgaa
     1081 ggggcggccc gtcggcatcc tgctgctcgg cctgtggtgc gccgcgcata cggttccctt
     1141 cggcctgctc gcggccgatg cgagcggcgg caaggcgttt ctcgcgtggg ttgcggtgat
     1201 cgtcgcgctg ctggcggccg ccggcctgct gatccgttcg cggatcgcgc actgggtcgt
     1261 cgtgcttcag gtcggggtcc acgtgttcgt gttctccgtg gccgcctggg cgttcctgtt
     1321 cgtggcgctg gcgtgggggt tgcacgcgtc ggagaccgcg gtcgtcgcgc tcatcttcgc
     1381 ctacctgtcg ttcacctgct gggcgctcct gtacctgttc tacccggacg tgctggacta
     1441 cttcgaagaa tcccgcagtt tcttccgtcc ggcctcgcac gacggcctgt gaccctctca
     1501 gccgccgccg gctgccgctg ccgcggcagg cgccgtccgg aaggtttatc ctatcgcgcg
     1561 ctcgtcgccc tgctgcggag ggatgtcatg cacgcgccgg ttcgatacgg tttggtggtt
     1621 ctcgctgtcg tgttcccgct gccgggcggc gccgcgtgtc cggacgacgc ggccgtggcg
     1681 gcctatgtgg ccgactatcg ggcggctcgc atgagccgcg gcttcggcaa cgatctcagc
     1741 ctcgccgagg cgcagtgcgc gaaggcgaaa ttgacgcggc aactgccgca ggtgcagggc
     1801 gccgtcgtcg ggtacaaggc ggcgttcacc aatccggcga tgtacgagcg cttcggcgtg
     1861 accggccccg cgtggggcgt gatgttcgcc aagatgatgg tcgacagcgg ggcgaagctg
     1921 cccgcccgct tcggggcgcg ccccctctac gaagccgact tcctggccgt ggtcaaggat
     1981 gctgggctgg ccgacgccag gacgcaactg gaagcgttgc agcacatctc cgcggtggtg
     2041 ccgttcatcg agctgccgga tctgatgctg gaaggcccgg ccaccggcgc cgcgctcatc
     2101 ggcaccaatc tcggcttccg cggtggcgcg ctgggcccgc gcgtcgcggt cgagccgacg
     2161 caggcgtttc tcgacgcgct ggcgaacatg accgtggtca tgaccgagga caagagcggc
     2221 aaggagctcg gccgcgccaa gggcgacatc ctgatgcagc atcccatcaa cgccgcgatg
     2281 tggctggcgc aagcgctgaa gaaggacggc gtcgcgctca agcccggcga cctgctgagc
     2341 ctcgggggct acatcccgcc ggcgcccaca caatccggga cgaccatcac cgtcaagtac
     2401 ctcgggctgc cgggaaaccc cgcggtcacg gtgcacctgg agtagtctgt caacccggga
     2461 atagtctgct cgaccccggg gagtagcccg ctgagcccag gagcaacctg ctcaccccgg
     2521 gagccggccg gtcacgccgg ctgcaccgcg ctttccaggc cctcgacgag ataggtgacc
     2581 agcgggtcgg gtttgccctt gaccgcctgc ggcggcatcg gccgcaggcg gaagcggccg
     2641 tcgagctcgc ggcgcacctc ttcggtgatc agcagatcga ccgacagctc gcgggtgagc
     2701 tgctccaccc gggaggcgac gttgatggtg tcgccgacca ccccgaactt gctcagctct
     2761 tcgttgccca tcacgcccgc cagcacctcg ccgcggtgga tgccgatccc gacctgcagc
     2821 tcgggcaacg cgcgggcgcg cagcgtgtcg ttgtagcgcg ccagcgcctc gcgcatcgcg
     2881 agcgcgccga acacggcgtc ctgcgcctgc cagggattgg gctccagcgc cccgaacagc
     2941 gccagcattc cgtcgcccat cagctcggtg acgcgaccgt ggtgcgcggt gatcgccgcg
     3001 ctcatcgcgc ggaagtagcc gttcaggatc ggcaccatct gctccgggtc catgcggtcg
     3061 gacagcgcgg tgaagccctt gaggtcggcg aacaggacgg tcaccgtgcg tcgcgtcggc
     3121 ttcagctccc cgccgggttc ggtgaggcgc tcgatcacgt cgtccggcgc aaagcgggcg
     3181 aaatgcagct gcagccgctc gagcttgccg tgcgtgtcct gcaccaggcg ctcgagctgc
     3241 cggatcctgc gcgactgctt cagaccgagc gccagcgcgc cgacgaaggc cgccagaacg
     3301 aacgcccaca ggaaaccggt ggtcattcga gcagcatcgc cacgcgcacc gtcgagttgg
     3361 ccagcgcggc gacgccgacg gcctccagcg ccgcctctcc gccgatcgcc tccgccagcg
     3421 cgcgcgtgcg cgcctggatc tggctcggct ggtagcgaac agtatcgcgc acccaggcaa
     3481 gcacggccgc ttcgcgcgcg tcgagctttg gcagggccag cgcggacagc gatgcctcca
     3541 cttctgcgtc gtccaatcct tctgcgtgta gcagcttgcg gctttcgggc tcacagaagc
     3601 ggcattccag gatgcgggcc accaccgcga acatgagcgc cttgacgcgc cgcgatagga
     3661 tgggcgattc gaatgcgccg cgcagcgtct cgttgagcag ataggcggcc gggtgtccga
     3721 ccagcggggc gatgacgccc gcgaagggct cgtgctcgcg gggcacgaaa tccgccggga
     3781 tcggcaccga gacgcgattc gccctggcga gcagcgggcg cagcagccgg ccgaacggcg
     3841 atcgcccgat gcgctcgtag ccggtcgccg gcggcaaggc gatgaacgtc gccacgcggt
     3901 tgccgaagca gttgttcacg atcaggaaag cggcctcggt gacctgctgc ggcgagaagc
     3961 ccacccggat cagctcctcg cgctgctcgc gggccggccg cgggttggag cgggccaggc
     4021 tgcggcagaa acgaatgaaa gcctggtcct tctcatccag ctccgcgagc tgcatctggc
     4081 gctcgatctg gccgatcacg cgttccgagt agccgaggat gcgcatgtgc gcgcgcgccg
     4141 ccccgtagca gtagcggcac gagttctcct gcgcgacgac caggaacacc aggtccgcca
     4201 tgcgcaccgg cagtgccttg atcgggtact gcgtccaggt cgcgaccgca cgccgcaacc
     4261 agggcaccgg cgccacgcgg cgcaggaaat ccgagctgcc gccgaagcgc tgcttcgcct
     4321 cggtttccca ttccgcgacc accacgggtg gaaccagcgg cggtccccat tcgatatcgc
     4381 tcagcaactg tgccatcatg cctccgtccg atgcatgcgc gctgcggcgc gttctcgcac
     4441 gccagcccgc acccgccgga tcgaaccggc gaaagcacga ttctaccgca ccatcctcgg
     4501 gccgcttcgc gcgcgaggcg ctgtggtatg gttgagcgtg tcaatcggca aggaggcccg
     4561 gcgatggcga atggcgcagg cacctgcttc gtggtcatgg ggttcggcaa gaagaccgat
     4621 ttcgagagcg gccgcacctt cgacctggac aagtcctacc gcaacctgat caagccggcg
     4681 gtcgaggcgg cggggtaccg gtgcgtgcgt gccgacgaga tcgtgcactc cgggctgatc
     4741 gacgtgccga tgtacgagca gctgctggcg gccgacatcg tggtggccga tctgtccacc
     4801 gccaacaaga acgcgttcta cgagctgggc attcgccacg cgctgcggcc gtacaccacc
     4861 atcgtgatcg ccgaggacgg cctgaagccg cctttcgacg tcaatcacgt cgtggtgcgc
     4921 cactacaagc atctgggcga ggacatcggc ttcgacgagg tgatgcgctt tcgcaagctg
     4981 ctcaccgagg cgatcaccgt gatcgccgcc cagccggtgg acaaacgcgt ggacagcccg
     5041 gtatatcgct tcgtcgccgg gctcaatccg cctgcgctgg ctgcggttgc ggcggcgccg
     5101 cccgcgccgg cggcggtgcg cgctccggcg gccgcgggcg gcgccacgca cagcgccttg
     5161 atgcagcagg tcgacgatgc ggtggcgcgc aaggacttcg tcgccgccaa gagcctgctc
     5221 acggtggtgc atgacatgat gcgcgccgag gcgccggacc gcggcgaaga tccttacatc
     5281 gttcagcgcc tggcgctgct cacctacaag agcaagcacc cgagcgagga agcgtcgctg
     5341 cgcgaggcgc tcgcgctgct ggccgggttc gacccggaca cctccaacga caccgaaacg
     5401 ctcgggctgt ggggcgcggt ccacaagcgg ctgtggtcgc tcaccggcga gcggccgcac
     5461 ctggacgcgt cggtccgggc gtacgcgcgc ggcttctact tgcgcaacga ctactataac
     5521 ggcatcaatc tcgccttcct gctcgacttg cgcgcctcgc gcgcggagcc cgccgacgcg
     5581 atcgcggacc atgtcctcgc gcgccgcgtc cgcaaggagg tgaggcagat ctgcgagaag
     5641 tggctggcgc agaatccgcc ggcggccgcg ccggcggacg cggggccgca ggcggcgcgg
     5701 caggcggccc aacaagcggc agagcatcgc tactgggtgc tcgccacgct cggcgaggcg
     5761 atgctgggtc tcggcgaccc cgccgcgcag cagcagctcg aggccgccta cgccgcggcc
     5821 ccgtacgcct ggatggcgga taccacgcgc gagcagatcg cgaagctgga accgctgctc
     5881 gccgactcgc ccttgaagta tctgaagccc gcggcgtaac cgggctgtca tcgcagcgtt
     5941 cgatcacgcg cgcacaatgc gcgccatcgc cgtcgccgcg cacggcatcg actattccac
     6001 acggtgtatg gacgatcgcg ctgcgcgggc ttagcattga gcgcatcgaa ccgatcgcgc
     6061 ttcgccgcga tcgaacctca cattttgtta ggtttcgatg actcgcgtca ccgccgccaa
     6121 cctgatcgga cgttcggccg ccttccagga gacggtgcgc ctggtccagc gcatcgccca
     6181 gaccgatgcg ccggtgctga tcgagggcga aaccggcacc ggcaaggagg tggcggcgcg
     6241 ggccattcat tactcgggcg tgcgccgcga ccggccgttc gtgccggtca actgcggcgc
     6301 gatcccggaa aacctgatcg agaacgagct gttcgggcac gtaggcggcg cctataccga
     6361 cgctcgcggc ccgcagttcg gcctgatcgc gctggccaac ggcggcacgc tgttcctgga
     6421 cgaggtggag acgctgtgcc cgaagggcca ggtggcgttg ctgcgcttcc tgcaggacct
     6481 caactatcgt ccgctcggca gccgccacga gcagagcgcc gatgtccgcg tcatcgccgc
     6541 caccaacatc aacctgtccg agctggtgca gagcaagcag tttcgcatgg acctgtacta
     6601 ccggctgcag atcctgttcc tgcgcatccc gccgctgcgc gagcgcgacg gcgacccggc
     6661 gctgctcgcc gagcacttcg cgcgcgcgtt cagcgcccgc tacggcggtc cggctacgcg
     6721 gttcgccgcg gacagcctgg cgttgctcgg cgggtacgcg tggcccggca acgtgcgcga
     6781 gctggagaat ctggtgcatc gcgctttcct gttggcgggc ggcgatgtca tctccatccc
     6841 acacgactgg ctcaccaacg agccgcgccc ggcggcggcc gacgcgcacc aggtctttcc
     6901 cctcgatctc gccttcagcg aggccaaggc gcggctgatc gatcagttcg agcgcgtgta
     6961 catcgcgcac gtgctgtccc agaccaacgg caacgtctcg caggccgcgc gccgctgcgg
     7021 caaggagcgc cgcgccttcg gcaagctgct gaagaaacac ggcatcgaca agcggttatt
     7081 cgagcgaccc acgcgggtcg cggcacaccc atagcgtctc gatgcaccgc atcttccgca
     7141 acgccgcgcg cggcaaggcc gacgcggttt tgcaacggcg ccgtcgcctt ctgcatacgc
     7201 ggggcggagc cgacccacgc cgacgtgaat gcgatcgcgc acaccaggaa tttcagcggt
     7261 ttgcgctgcg tgcgcgcatg gcatgcgctt agcccgtcag gcgcacacat ccccaggggc
     7321 cgcccgatgc cggaccttgc acccgaggtg cgcgcgctcg ttagtcgcat ccggcagtac
     7381 atcctcacgc gttcgcaagc ggcggacagc gtcaagggag tagcgggcgc atggctggag
     7441 ctgagcggcg aggaggcgtg gctgccggtg gtggagcagg cgctcgagca cctggtggcc
     7501 accggcgaga tgcaacgcca gcgcttggcg gacggaacgg tgatgtacag cgttgccacg
     7561 cgcatccggc actaggaatt cgggcggagc tcaacagcta tgcggaactg tgccagaaca
     7621 tcttgattca ctcgcggccc gacctgcctg gcggcgtttg cgcatcgcgc ctatactcgg
     7681 ttgcatcgcg aacgcacggg agggggcgcg catggcgaag gcgaccggca ggaaggcggc
     7741 caagaagaag ggaagcggca agaaggcgac cgagaagaaa gcgaccaaga gccccaagaa
     7801 ggcgagcaag acaatgggca aagcggcctg gacggtgatg gtgtacctcg ccggcgacaa
     7861 cgacctggac agcgccgggg tggtggacct cggcgagatg aaggaagtcg gctcgagcga
     7921 tcggatcaat ctgctggccc agttcgatcg cgccgggccg cgcatcgaga cccggcgttt
     7981 cctgctgcgc aagggcacgc cgctcggccg ggacctggtc aagagcctgg gcgagaccaa
     8041 catgggcgat cccaagatcc tgaaagattt cctggtctgg ggcgcgaaga actatccggc
     8101 cgagcgctac atggtggtga tctggaacca cggcgcgggc tgggacgacg agaacgtcta
     8161 ccgcgcggtg cgcggcgggc tcaagcgcgg cgtggcgtac aagaagcacg cggtgggccg
     8221 cccggcgcgc ggcgccaagc gggcggtgcc gctgagccgg ctgcgctcgg tgtccaagcg
     8281 caagttcaag cgcgcgctgt tctcttccac tgtccagcag gcggtcgaaa agcgcgccat
     8341 cgccttcgac gacgacgccc aggacttcct cgacaacatc gagctcaaac gcgttctcac
     8401 cggcgcgcgc aaggtgttcg gcggcaagat cgacgttctg ggcatggacg cctgcctgat
     8461 gaacatggcg gaggtcgcct accaggtgcg caacaccgcc caggtgctgg tgggctcgca
     8521 ggaagtggag cccgccgacg gctggcctta ccacaccatc ctgcgcgacc tcgctaagaa
     8581 gccgggcatg acgccggcgg agctgggcaa ggtgatcgtg ggccgctacc tcgcctccta
     8641 cggcgccaac gagggcgtca cccagtcggc gctggatctg tcgctgtgcc gcggcctcga
     8701 agcggccatc gacaagctcg ccgcggtgct gatcggcgcg ctgccgggcg acgacgcccg
     8761 gctcggcttg ctgcaggcgc gccaggcggt gcgcgactac gacacgcgcg actacatcga
     8821 cctggtgcac ttctgtcagc tgctgcgcaa gttcagcggc agcgccgcga tccacgccgc
     8881 gtgcgatgcg gtcgacggcg ccgccgtcaa gctggtgctc gcctccggct tcaagggcac
     8941 aggcgtgaag aactcgaacg gcgtttcgat ctacttcccg gaaggatcga tctcgccgct
     9001 gtacgccaag ctcgacttcg cccgcaacaa caagtggaac gagatgctga gggcgtactt
     9061 cgacgcggtg agcgcgtagc ccgcgcttgc ctaatgcgca cctagagcag actccacctg
     9121 cccgggccag tactgcattt gcatcgcacg gtacagggaa agtgccgtag ttaggtgctc
     9181 ccgcgccttg ccgcgctcac gagcgcgtgc ttcgatctgg cctagaccca ggtggcaatg
     9241 ggcgaccagc ggctgcagtc cgagctggcc ggcccctcca agagcagctt ggtaggcttc
     9301 acgtgctgca gccgcgtcgc tgtcggaggc gcgcgctagg atgcgtccac gcacatagag
     9361 agcccaagct ttgcgggccg ggaaatcggc atcgtcagcg atcttcaggg catgctctgc
     9421 gcaatcccag gcgcgagcat cctgacccgt gaggctgtac gcgtcggcga catgcatgag
     9481 gcacaccacg ccgaaataga cgaaaccgcc ggcctccgcc agaccccacg cgcgctcgag
     9541 caatgggatc gcatcccgag gccgttgcat caggttgtat acaggcccga gcccgcaggc
     9601 ggcatgaatg gtcgccccag gaaaatccgc ttcctccc
//
LOCUS       DASPGE010000159         3269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_161623, whole genome shotgun sequence.
ACCESSION   DASPGE010000159 DASPGE010000000
VERSION     DASPGE010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161623"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VD791_05045"
     CDS             complement(<1..481)
                     /locus_tag="VD791_05045"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HXV07372.1"
                     /translation="MSKHLEWLKQELAQWRAEGLVDEPLAQRILARYPAAAERNWSRI
                     IFSAIGAVLVGLGVILFFAYNWQALPKAAKLALALGALALAHGSALALARRSGAGRGV
                     VEGLHALGTMLFGAGIWLVAQIYHIDEHYPNAFLVWSAGALAMAWAMPSIVQALLALF
                     "
     gene            complement(513..968)
                     /locus_tag="VD791_05050"
     CDS             complement(513..968)
                     /locus_tag="VD791_05050"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HXV07373.1"
                     /translation="MIQTRHLFTLTADVPGIVELGATPQGTRRIATVSGGTFRGERLS
                     GKIQAVPGGDWILVRPDGATILDVRVTLETDDRQLIYMTYRGVRHGPAEVMARLAKGE
                     AVDPASYYFRTTPVFETAAPKYDWLNRIVSVATGRREARGPVYEVYEVL"
     gene            1087..1863
                     /locus_tag="VD791_05055"
     CDS             1087..1863
                     /locus_tag="VD791_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV07374.1"
                     /translation="MSSKERSYLLTDAGRQAWENQDLSVPEDYRRILWLMDFHGKEAV
                     VGELLRRYPAKVLDEWLMEMEQLGLIEPATDGTEGAAFSTRATDRTGNMDQAALKRNG
                     QSASEHLTHTGAYIAADRLSSRPAHRKGPTECVVLIVEDDPDQLALAELRVSMAGYKV
                     RIATSVNAFLHSMLDDGAPDLLLLDVFLPDGNGFDLLEKMRRHAVLGALPIVMLTSEN
                     KPEYIGKGLLLGADGYIIKPYTKNILADVIRRVLKHDGSA"
     gene            2050..>3269
                     /gene="dctP"
                     /locus_tag="VD791_05060"
     CDS             2050..>3269
                     /gene="dctP"
                     /locus_tag="VD791_05060"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXV07375.1"
                     /translation="MRGIPLKRLPAIAVAAAFVAGVATLAAVPQAAHAQAKPVIMRFA
                     ADFPPPPHPAGLAMKYFAERLPKVIPGSEARLYFAGALYTVPEAYEAMRQGNLEMTWM
                     QIGKAAPVDSYMLSVVGPGILTTVGAVDNIDKTSTFQMLVERMAKNQQVKVFGVGHMS
                     FGMGVGGKKRYAAPADFVGKKVRSMGPAENPVLEAWKANPVVMAFGEVPSAMESGVID
                     GLMTSLGGWNSMREQAPYYTMGGAGAFVGDYYMVSASRRWWDRLSPAQREAMEKLIAE
                     TIQVQKEYNWCFDKIAYDKYGTKDPSKPGVYWMTPEEVNAMVGAMGSAAVNYVKSKTP
                     PAAAPWVDKFVQEGRELSKKNPSGTSWIEKVDCSKHASKIIIRSMEKADAAWRAFQDR
                     FLAHAAALVLLGATL"
BASE COUNT          569 a         1075 c         1162 g          463 t
ORIGIN      
        1 ggaacagcgc gagcagcgcc tggacgatcg agggcatcgc ccacgccatg gccagcgcgc
       61 ccgccgacca gaccaggaac gcgttcgggt agtgctcgtc gatgtggtag atctgcgcga
      121 ccagccagat gccggcgccg aacagcatgg tgccgagcgc gtgcagccct tccacgacgc
      181 cgcgcccggc gccggatcgg cgcgcgaggg ccagcgcgct gccgtgcgcg agggcgaggg
      241 cgcccagcgc cagcgcgagc ttggcggcct tgggcagcgc ctgccagttg taggcgaaga
      301 acaggatcac gcccaggccc accagcaccg cgccgatggc cgagaagatg atgcggctcc
      361 agttgcgctc ggcggcggcg gggtagcgcg ccaggatgcg ctgcgcgagc ggctcgtcca
      421 cgagcccctc ggcacgccac tgcgccagct cctgcttcag ccactcgagg tgcttgctca
      481 agccgcgcct ccccggtact cgctgcagga tatcagagca cttcgtacac ctcgtagacc
      541 ggcccgcgcg cctcgcgccg gcccgtggcc accgagacga tgcggttcag ccagtcgtat
      601 ttcggcgcgg cggtctcgaa caccggcgtg gtgcggaagt agtagctcgc cggatcgacc
      661 gcttccccct tcgccagccg cgccatgacc tccgccgggc catggcgcac gccgcggtag
      721 gtcatgtaga tgagctgccg gtcgtccgtc tcgagcgtca cgcgcacgtc gaggatcgtc
      781 gccccgtcgg gtcgcaccag gatccagtcg ccgccgggaa cggcctggat cttgccggac
      841 aggcgctcgc cgcggaacgt gcccccgctc acggtggcga tgcgccgcgt gccctggggc
      901 gtggcgccga gctcgacgat gccggggaca tcggcggtaa gggtgaagag gtgtcgggtc
      961 tggatcatgt ggcgcgctcc atcggcggtg gagggaggat ggtactgaaa atcgctcccg
     1021 ggacgcggcg caagccggac agccggggca taatcgcgcc acacatcacg aagaagcccg
     1081 gggaagatga gcagcaagga gcgcagctat ctgctgaccg acgccggccg gcaggcctgg
     1141 gagaaccagg acctgtccgt gcccgaggac taccggcgca tcctgtggct gatggacttc
     1201 cacggcaagg aggcggtggt gggcgagctg ctgcgccgct acccggcgaa ggtgctcgac
     1261 gagtggctga tggagatgga gcagctcggg ctgatcgagc ccgcgaccga cgggacggag
     1321 ggcgccgcgt tttccacgcg cgccaccgat cgcaccggca acatggacca ggcggccctc
     1381 aagcgcaacg gccagtcggc cagcgagcac ctcacccata caggggcgta catcgcggcg
     1441 gaccgcctga gctcgcgccc ggcgcaccgc aaggggccga cggaatgcgt cgtgctgatc
     1501 gtggaggacg acccggacca gctggcgctc gccgagctgc gcgtcagcat ggccggctac
     1561 aaggtacgca tcgcgacttc ggtgaacgcg ttcctgcaca gcatgctgga cgacggcgcg
     1621 cccgacctgc tgctgctgga cgtcttcctc ccggacggca acggcttcga cctgctggag
     1681 aagatgcgcc gccacgcggt gctcggcgcg ctgccgatcg tcatgctcac ctcggagaac
     1741 aagcccgagt atatcggcaa ggggctgctg ctcggcgccg atggctacat catcaagccc
     1801 tatacgaaga acatcctcgc cgacgtcatc cgccgggtgc tgaagcatga cggcagcgcc
     1861 tagccggtga acgacgatcg cggctgtcac cgcgtgatgc aacgcgtggt gtacgccgga
     1921 gatgtgcccg cgtaagcggg ttgcggcatt gcagcaattg accgcgccgc ttgaccgggc
     1981 gccgtacggt gagatacggt cgcctggcta cgtgtggatt caaatcacga gattcgagga
     2041 ggaggtgtca tgagaggtat cccgctgaag cgtctgcccg caatcgccgt cgccgccgca
     2101 ttcgtggcag gcgtggcgac gctcgccgca gtcccgcagg ccgcgcacgc gcaggccaag
     2161 cccgtgatca tgcggttcgc cgccgatttc ccgccgccgc cgcatcccgc ggggctggcc
     2221 atgaagtact tcgccgagcg cctgcccaag gtgattccgg gcagcgaggc gcggctgtac
     2281 ttcgccggcg cgctgtacac ggttcccgaa gcctacgagg ccatgcgcca gggcaacctg
     2341 gagatgacct ggatgcagat cggcaaggcg gcgccggtcg actcctacat gctgtcggtg
     2401 gtcgggcccg gcatcctgac gacggtcggc gcggtcgaca acatcgacaa gacctcgacc
     2461 ttccagatgc tggtcgagcg catggcgaag aaccagcagg tcaaggtgtt cggcgtcggc
     2521 cacatgagct tcggcatggg cgtaggcggc aagaagcgct acgccgcgcc cgcggacttc
     2581 gtcggcaaga aggtgcgcag catggggccg gcggagaacc cggtgctcga ggcgtggaag
     2641 gccaatcccg tggtgatggc cttcggcgag gtgccgagcg ccatggagtc gggcgtcatc
     2701 gacggcctga tgaccagcct gggcggctgg aacagcatgc gcgagcaggc gccgtactac
     2761 accatgggcg gcgcgggcgc gttcgtcggc gactactaca tggtgagcgc gagccggcgc
     2821 tggtgggacc gcctgagccc cgcgcagcgc gaggcgatgg agaagctgat cgccgagacc
     2881 atccaggtgc agaaggagta caactggtgc ttcgacaaga tcgcctacga caagtacggc
     2941 accaaggatc cctccaagcc gggcgtgtac tggatgaccc ccgaagaggt caacgccatg
     3001 gtgggcgcga tgggcagcgc cgcggtcaac tacgtgaaga gcaagactcc gccggcggcc
     3061 gcgccctggg tcgacaagtt cgtgcaggaa gggcgcgagc tgtcgaagaa gaatccgagc
     3121 ggcacctcct ggatcgagaa ggtggactgc tcgaagcacg catcgaagat catcatccgg
     3181 tcgatggaga aggccgacgc agcctggcgt gcgttccagg acaggttcct ggcgcacgcc
     3241 gctgcgctcg tgctgctcgg ggcgacgct
//
LOCUS       DASPGE010000160        11601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1449527, whole genome shotgun sequence.
ACCESSION   DASPGE010000160 DASPGE010000000
VERSION     DASPGE010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11601
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449527"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VD791_05065"
     CDS             complement(<1..207)
                     /locus_tag="VD791_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07376.1"
                     /translation="MRTMLLLPLVAAATIAACAGNAAAADPEPPAQAATLGEAITGGK
                     LLLNLRPRWEHVEQDNKPEDGDAIT"
     gene            477..2417
                     /locus_tag="VD791_05070"
     CDS             477..2417
                     /locus_tag="VD791_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HXV07377.1"
                     /translation="MPQTTRKLGSKLTAILIAYLVVAVGAVGLTLLTSWQLEGGAAAV
                     NDMGSQRMRSYHIALLLGQANAPGADRARLTQAVRAEMTQFERVLADLERGDSSRPMM
                     LPRDPDIRSRFDALKREWNTAMKPGIERSIAAAVDRANGDAAPSAAEYLSATQGFVAR
                     IDALVLDIEQAISDNTRLLRSLQFGLIALSVIGTVALIYLMYLLIIRPVTGLEEGMRR
                     MEGGDFGARLPVESRDELGALAAGFNRMAAHLQGLYASLERRVAEKTRTLEDKNKELG
                     TLYEIAALLSQPAALDNLCRDFLRKLIARLGARGGAVRLVEADTGRLHMFVHQGIGTD
                     LVEAERCLERGDCLCGQGLQRQHPSVHVLVRRPYDKEPYRCREAGFATVGVFPIRFRD
                     QALGVFNLYFAEPREFDPQQRFMLETLGQHLGIAVENQRLIAREKEMAISEERNLLAQ
                     ELHDSIAQALAFLNLQAQMLQESLASGRDDQAREELARIREGIQESYDDVRELLVHFR
                     TRIAQTDIESTIAAALERFEGQTGIRTAFSQSGVGMPLSPETQLQVMHILQESLSNAR
                     KHSGAKRVEVEMQRGPVYRFRLRDDGQGFDPHEQSSDLHVGLRIMRERAHRVGGTLRV
                     DSRPGAGTEVVLTLPVLQEQAA"
     gene            2428..3090
                     /locus_tag="VD791_05075"
     CDS             2428..3090
                     /locus_tag="VD791_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV07378.1"
                     /translation="MDAIRVLIVDDHTLFRSGLKALLARQPGFEVVGEAADGLDGIKR
                     AKALKPDVILLDLHMRGLSGREAVKTLSEEVPESRVLMLTVSEDADDLIETLRRGASG
                     YLLKNIDTQMLLDSIRRAAEGEAVVSAEMTSKLVRGLKAGPRPEAQPADRERLSPRER
                     EILGFLAKGASNKEIARELEVAESTVKIHVQHILRKLNLTSRVQAAVYAVESGLIEQP
                     RP"
     gene            3326..4606
                     /locus_tag="VD791_05080"
     CDS             3326..4606
                     /locus_tag="VD791_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HXV07379.1"
                     /translation="MATPRYQRVSAAHQRVSVLVASTAAFTVCFMVWMMFGVIGIPIQ
                     KTLGLNATEFGILTATPVLTGSLIRVPLGMWTDKYGGRIVMFLLMLATVVPIWLISYA
                     TAFWHYLVLGLFVGVAGGSFSVGTPYVARWFDKQHQGFAMGVYGAGNSGAAVNKFIAP
                     ALVVAYGWFVVPQVYAVSMLLTAVLFWFFSYSDPKHLVDAKITWREQLAAMKDPKVWK
                     YSQYYSIVFGGYVALALWMTQYYVGEYELDIRVAALLAACFSLPGGVLRAIGGWLSDK
                     YGAHQVTWWVLWVSWICLFILSYPQTDFTIFTVDGPKTFHIGLNVWVFTLLMFVIGTA
                     WAFGKASVFKYISDDYPHNIGVISGIVGLAGGLGGFVLPVMFGALVDLTGIRSSCFML
                     MYGVVWVSLIWMYFTEVRRAEVIGPRAQPFSLRG"
     gene            4659..6083
                     /locus_tag="VD791_05085"
     CDS             4659..6083
                     /locus_tag="VD791_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015514 - nitrite efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015707 - nitrite transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HXV07380.1"
                     /translation="MSLDTAAQRSREGKRGRVLTLWTPEDRAFWEKEGQAIANLNLWI
                     SVPCLFLAFAVWQLWSVVAVNLPVLGFKLTTNQLFWLAAAPALSGATLRIFYSFMVPI
                     FGGRRWTALTTASLLIPAVGIGLAVQDPTVSFPTLLILALLCGFGGGNFSSSMANISF
                     FFPKERKGSALGVNAGLGNLGVSSVQFLAPLVVTMPIWGVLSGDPQTIVDKSGQTLQI
                     WAQNAAWIWVPFIALGAFFAWFGMNDIADARASFSDQAVIFRRKHNWLMCWLYLGTFG
                     SFIGYAAGFPLLIKSQFPEVNPLHYAWMGPLVGAVIRPFGGWLSDKLGGARVTFWNFI
                     VMELAVIGVLYFLPSGGSGGDFAGFLGMFMLLFLTTGIGNGSTFRMIPIIFLTERTRA
                     AGKDPAAQEQATRDGNKEAAAVLGFSGAIGAYGGFFIPKSYGTSISMTGGPEAALYVF
                     IVFYLTCIATTWWFYARRNAQMPC"
     gene            6197..9955
                     /locus_tag="VD791_05090"
     CDS             6197..9955
                     /locus_tag="VD791_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HXV07381.1"
                     /translation="MSHFLDRLTFFTRDKESFSGGHGVVTDEDRSWEDAYRKRWQHDK
                     IVRSTHGTNCTGSCSWKIYVKGGVVTWETQQTDYPRTRPDMPNHEPRGCSRGASYSWY
                     LYSANRLKYPMVRGRLVRLWREARKHLGPVEAWASIVQDPVKAKSYKSVRGMGGFVRS
                     TWDEVQEIVAAANVYTIKQHGPDRVIGFSPIPAMSMVSYAAGSRYLSLIGGVCMSFYD
                     WYCDLPPSSPQTWGEQTDVPESADWYNSTFLIAWGSNVPQTRTPDAHFFTEVRYKGAK
                     VVAVTPDYAEVSKLADLWLHPKQGTDAALAMAMGHVILKEFHVDRQAPYFADYVRRYT
                     DMPLLVRLNKRGERYIADRFLRASDFGDKLGQANNPEWKTLALDEAAGAGAPSVVVPN
                     GSIGFRWNQKPGEDQGKWNLEDRDARSAAPVKLRLSLIDARDTVAPVAFPYFGGIAHE
                     HFRHNDQGSEVLVRNVPVRKLQLADGETLVATVFDLLLANYGIDRGLGGERLARSYDD
                     DVPYTPRWQEAITGVKAQDVITVARQFADNAEKTQGKSMIIIGAAMNHWYHCDMNYRG
                     IINLLMMCGCVGQSGGGWAHYVGQEKLRPQTGWTALAFALDWHRPPRQQNSTSFFYAH
                     TDQWRYEKLQVHEILSPLADPNDFTGSLVDFNVRAERMGWLPSAPQLQTNPLRVCRDA
                     AQAGLDPRDYAVQALKSGSLKLACEDPDDPANFPRNLFVWRSNLLGSSGKGHEYFLKH
                     LLGTRHGVQGKDLGAEGGSKPEEVAWHDDAPEGKLDLLVTLDFRMSTTCLYSDVVLPT
                     ATWYEKNDLNTSDMHPFIHPLSCAVDPVWEARSDWEIYKGIAKKFSEVAVGHLGVEKE
                     LVLTPLMHDTPAELAQALDVKDWKRGECDLIPGKTAPQMTVIERDYPNTWRKFTALGP
                     LMTKAGNGGKGIAWNTEHEVRNLAALNQVVIEDGITKGMPRIDTDIDACEVILMLAPE
                     TNGEVAVKAWEALSKVTGREHEHLAIPREDDKIRFRDVQAQPRKIISSPTWSGLESEK
                     VSYNAGYTNVHELIPWRTLTGRQQFYQDHKWMLGFGEGLCAYRPPVDTRSVAPLLGKR
                     GNGNPEVLLNFITPHQKWGIHSTYTDNLLMLTLSRGGPIVWVSEVDARKMNVEDNDWI
                     ELYNINGAITARAVVSQRVAQGMCLMYHAQEKILNVPGSEITGTRGGIHNSVTRTVTK
                     PTHMIGGYAQLAYGFNYYGTIGTNRDEFVIVRKMQTIDWMDEPAENVL"
     gene            9965..11476
                     /gene="narH"
                     /locus_tag="VD791_05095"
     CDS             9965..11476
                     /gene="narH"
                     /locus_tag="VD791_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HXV07382.1"
                     /translation="MKIRAQIGMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNV
                     ETKPGIGYPKEWENQQRWSGGWVRKASGRIEPRIGGKWQVLMKIFANPHLPEIDDYYE
                     PFTFDYEHLHTAPEMKAPPVARPRSLVSGKPLEKIEWGPNWEEILGGEFAKRSQDSNF
                     EAVQKDIYGQFENTFMMYLPRLCEHCLNPACVAACPSGSIYKREEDGIVLVDQDKCRG
                     WRMCVSACPYKKIYYNWQSGKAEKCTFCYPRIEVGQPTVCSETCVGRIRYLGVMLYDA
                     DRIEEAASAEHEQDLYEAQLAIFLDPRDREVIERARADGVPEAWIEAAQASPVHRMAI
                     EWKIAFPLHPEYRTLPMVWYVPPLSPVQGAAEAGHVGFKDELPDIDALRIPVRYLANM
                     LTAGDTEPVSLALKRMFAMRIYQRGRHVDGTLHTDVLRQVGLTANQVEEMYRLLAIAN
                     YEDRFVIPTTHREYAEDAFDLRGSCGFSFGNGCSHGMSEPKLFGAPAPRKEMA"
BASE COUNT         1952 a         3839 c         3926 g         1884 t
ORIGIN      
        1 ggtgatcgca tcgccgtcct cgggcttgtt gtcctgctcc acatgctccc agcgcgggcg
       61 cagattcagc agcagcttgc cgccggtgat cgcctcgccc agcgtggcgg cctgcgccgg
      121 cggctcgggg tcggctgcgg ccgcgttgcc tgcgcatgcg gcgatggtcg cagccgccac
      181 cagcggcaac agcagcattg ttctcattct cgtctccatc aaatccggcc ggcggcggcc
      241 agggccgggc gggccgcgca cgcgttcggg ccggtatgct gggcagcatg gcgcgctggc
      301 cggcggcttc ctattctgcg aaggagcgag cgcgggcttc tgaacggtgt agcccgcgtg
      361 cggagcggcg cgcgacggcg tacgccggtc ggcctacacc ttcgcgtgcg ccgagcgcac
      421 cggccgcgga tgccgggcgc gctctgctat agtgcggcca ccttccgcct ccgcccatgc
      481 cgcagacgac gcgcaagctc ggcagcaagc tcaccgcgat cctgatcgcc tacctggtgg
      541 tggcagtcgg cgcggtgggc ctgacgctgc tcacgtcctg gcagctcgaa ggaggcgcgg
      601 ccgcggtgaa cgacatgggc agccagcgca tgcgctcgta tcacatcgcg ctgctgctcg
      661 gtcaggcgaa cgcgcccggg gccgaccgcg cccgcctgac gcaggcggtg cgcgcggaaa
      721 tgacgcagtt cgaacgggtg ctggccgacc tcgaacgcgg cgactcctcg cggccgatga
      781 tgctgccgcg cgacccggac atccgcagcc gcttcgacgc gctgaagcgg gaatggaaca
      841 ccgccatgaa gcccggcatc gagcgctcga tcgcggccgc ggtagaccga gcaaatggag
      901 acgctgcgcc gtcggcggcc gaatacctca gcgcgacgca agggttcgtg gcgcgcatcg
      961 acgcgctggt gctcgacatc gagcaggcga tctcggacaa cacgcgcctg ttgcgatcgc
     1021 tgcagttcgg actgatcgcg ctcagcgtga tcgggacggt ggcgttgatc tatctgatgt
     1081 acctgctgat catccggccg gtgaccggcc tcgaggaggg catgcggcgc atggagggcg
     1141 gcgacttcgg cgcgcgcctg ccggtggaat cgcgcgacga gctcggcgcc ctggcggcag
     1201 gcttcaaccg catggctgcg cacctgcagg ggctgtatgc cagcctggag cgccgcgtgg
     1261 cggagaagac ccgaaccctg gaggacaaga ataaggagct gggaacgctg tacgaaatcg
     1321 ctgcgctgct gagccaaccc gcggccctgg acaatctatg ccgggacttc ctgcgcaagc
     1381 tcatcgcgcg cctgggcgcg cgcggcggcg cggtgcgcct ggtcgaagcc gacaccggca
     1441 ggctgcacat gttcgtgcac cagggcatcg gcaccgatct ggtcgaggcc gagcgctgcc
     1501 tggagcgcgg cgactgcctg tgcggccagg gcctgcagcg ccagcacccg agtgtgcacg
     1561 tgctggtgcg ccgcccctac gacaaggagc cgtaccgctg ccgcgaggcc ggcttcgcca
     1621 ccgtcggcgt gttcccgatc cgcttccgcg accaggcgct gggggtgttc aacctgtact
     1681 tcgccgagcc gcgcgagttc gacccgcagc agcggttcat gctggagacg ctcggccagc
     1741 atctgggcat cgcggtcgag aaccagcgcc tgatcgcgcg cgaaaaagag atggcgatct
     1801 cggaggagcg caacctcctg gcccaggagc tgcacgacag catcgcccag gcgctggcgt
     1861 tcctcaacct gcaggcgcag atgctgcagg agtcgctcgc cagcggccgg gacgaccagg
     1921 cacgcgagga gctggcgcgc atccgcgagg gcatccagga gagctacgac gacgtgcgcg
     1981 agctgctggt gcacttccgc acgcgcatcg cgcagaccga catcgagagc accatcgctg
     2041 cggcgctcga gcgcttcgaa ggtcagaccg ggatccgcac cgccttcagc cagtcgggcg
     2101 tgggcatgcc gctgtcgccc gagacccagc tccaggtcat gcatatcctc caggagagcc
     2161 tgtccaacgc acgcaagcac tcgggcgcca agcgcgtcga ggtggagatg cagcgcgggc
     2221 cggtgtatcg cttccggctg cgcgacgacg gccagggctt cgatccgcat gagcagtcgt
     2281 ccgacctgca cgtggggctg cgcatcatgc gcgagcgcgc gcaccgggtg ggcggcacgc
     2341 tgcgcgtcga ttcccggccg ggcgcgggca ccgaggtggt gctcacgctg ccggtgctcc
     2401 aggaacaggc cgcctgagat cctgcggatg gatgcgatcc gcgtgctgat cgtcgacgac
     2461 cacacgctgt tccgcagcgg gctgaaggcc ctgctcgcgc gccagccggg cttcgaggtg
     2521 gtgggcgagg cggccgacgg cctggacggc atcaagcgcg ccaaggcgct caagcccgat
     2581 gtcatcctgc tcgacttgca catgcgtggg ctgtcgggcc gcgaagcggt gaagaccctg
     2641 agcgaggagg tgcccgagtc gcgcgtgctg atgctcaccg tgtcggagga cgccgacgat
     2701 ctgatcgaga cgctgcgccg gggcgcgagc ggctatctgc tgaagaacat cgacacgcag
     2761 atgctgctcg attcgatccg ccgcgccgcc gagggcgaag ccgtggtgtc ggcggagatg
     2821 accagcaagc tggtgcgcgg cctcaaagcc ggcccgcgcc ccgaggcgca gccggccgac
     2881 cgcgaacggc tgagcccgcg cgagcgcgag atcctcggct tcctggccaa gggcgccagc
     2941 aacaaggaga tcgcccgcga gctggaggtc gccgagagca cggtcaagat ccacgtgcag
     3001 cacatcctgc gcaagctcaa tctcaccagc cgcgtgcagg ccgcggtata cgcggtggag
     3061 tctgggctga tcgaacagcc caggccgtag ccacaccgca ttctccccgc ctctcacgag
     3121 gcgggtgccc gcgaagcggg cggggcggtg tggtttgtag cctccagtcc tttttcgtac
     3181 tcctttcgaa ctacccccac ccttccccgc cgccgccgct gcctaggcat ttcacggccg
     3241 gcggctcctc cctccgcaga atgggcgcgg cgcactgcgc cgcgtacgct gcgcctgcat
     3301 cggccaagaa cggagaagaa aaagcgtggc aactccgagg taccagcgag tgtcggcggc
     3361 gcaccagcga gtctcggtgc tggtcgcgag cacggcggcg ttcaccgtgt gcttcatggt
     3421 gtggatgatg ttcggtgtga tcggcatccc gatccagaag acgctgggcc tgaacgccac
     3481 cgagttcggc atcctcaccg ccacgccggt gctgaccggg tcgctgatcc gggtgccgct
     3541 gggcatgtgg accgacaagt acggcgggcg catcgtcatg ttcctgctga tgctcgccac
     3601 ggtggtgccg atctggctga tctcctacgc caccgcgttc tggcactacc tggtgctcgg
     3661 cttgttcgtc ggcgtggcgg gcggttcctt ctcggtgggc acgccgtatg tcgcgcgctg
     3721 gttcgacaag cagcaccagg ggttcgcgat gggcgtgtac ggcgccggca actccggcgc
     3781 ggcggtcaac aagttcatcg cgcccgcgct ggtggtggcc tacggctggt tcgtggtgcc
     3841 gcaggtgtac gccgtctcca tgctgctgac cgccgttctg ttctggttct tcagctacag
     3901 cgaccccaag cacctggtgg acgccaaaat cacctggcgc gagcaactcg cagcgatgaa
     3961 ggacccgaag gtgtggaagt acagccagta ctactccatc gtgttcggcg gttacgtcgc
     4021 gctggcgctg tggatgacgc agtactacgt cggcgaatac gagctggaca tccgcgtcgc
     4081 ggcgctgctg gccgcgtgct tctcgctgcc gggcggcgtg ctgcgcgcca tcggcggctg
     4141 gctgtcggac aagtacggcg cgcatcaggt gacctggtgg gtgctgtggg tgagctggat
     4201 ctgcctgttc atcctgtcct atccgcagac cgacttcacc atcttcaccg tggacggtcc
     4261 caagacgttc cacatcggcc tgaacgtgtg ggtgttcacc ctgctgatgt tcgtgatcgg
     4321 cacggcgtgg gcattcggca aggcctcggt gttcaagtac atctcggacg actatccgca
     4381 caacatcggc gtgatctccg gcatcgtcgg gctggccggc ggcctgggcg ggttcgtcct
     4441 gccggtcatg ttcggagcgc tggtggatct gaccggcatc cgttcgagct gcttcatgct
     4501 gatgtacggc gtggtctggg tttcgctgat ctggatgtac ttcacggagg tgcgccgcgc
     4561 cgaggtgatc gggccgcgcg cgcagccctt cagcctgagg ggttgagccg gagcgaggcg
     4621 cgcgccgccg cgcgcgattt tgatcgagga gcgatgccat gagcctggat accgccgccc
     4681 aacgcagccg cgaaggcaag cggggtcgcg tgctcaccct gtggacgccg gaagaccgcg
     4741 cgttctggga gaaggaaggc caggccatcg ccaatctcaa cctgtggatc tcggtgcctt
     4801 gcctgttcct ggccttcgcc gtgtggcagc tgtggagcgt ggtggcggtg aacctgccgg
     4861 tgctgggctt caagctcacg acgaatcagc tgttctggct ggcggcagcg ccggcgctct
     4921 cgggcgcgac gttgcgcatt ttctactcct tcatggtgcc gatcttcggc ggccggcgct
     4981 ggacggcgct gaccaccgcg agcctgctga tccccgctgt gggcatcggc ctcgcggtcc
     5041 aggacccgac ggtgagcttt cccacgctgc tgatcctggc gctgctgtgc ggcttcggcg
     5101 ggggcaactt ctcctcgtcg atggccaaca tcagcttctt cttcccgaag gagcgcaagg
     5161 gctcggcgct gggggtgaac gccgggctcg gcaacctggg cgtgtcctcg gtgcagttcc
     5221 tggcgccgct ggtggtgacc atgccgatct ggggcgtgct ctccggcgac ccgcagacca
     5281 tcgtcgacaa gtcggggcag acgctgcaga tctgggcgca gaacgccgcc tggatctggg
     5341 tgccgttcat cgcgctcggt gcattcttcg cctggttcgg catgaacgac atcgccgacg
     5401 cccgggcgtc gttctccgat caggcggtga tcttcaggcg caagcacaac tggctgatgt
     5461 gttggctcta cctggggacc ttcggctcgt tcatcggcta cgccgccggc ttccctttgc
     5521 tcatcaagtc gcagttcccg gaagtcaacc cgctgcacta cgcctggatg ggaccgctgg
     5581 tgggcgcggt gatccggccc ttcggcggct ggctttcgga caagctcggc ggcgcgcggg
     5641 tcacgttctg gaatttcatc gtgatggagc tggcggtgat cggcgtgctg tacttcctgc
     5701 cctccggcgg gtcggggggc gacttcgccg gcttcctcgg tatgttcatg ctgctgttcc
     5761 tgaccaccgg catcggcaac ggctcgacct tccgcatgat cccgatcatc ttcctcaccg
     5821 agcgcacgcg cgccgccggc aaggaccccg ccgcgcagga gcaggcgacc cgggacggta
     5881 acaaggaggc cgccgcggtg ctgggtttct ccggggcgat cggcgcctac ggcgggttct
     5941 tcatcccgaa gagctacggc acctcaatct cgatgaccgg cggcccggag gcggcgctgt
     6001 acgtgttcat cgtcttctac ctcacctgca tcgcgaccac gtggtggttc tacgcgcggc
     6061 gcaatgcgca gatgccatgt tgaaagacgt gattcgtgaa tcgtgattgg tgattggtgc
     6121 gaggctatta cgaatcacga gtcacgaatc acgaagtcac ggattctaga gtcacgtgcg
     6181 acgctaggag cacaccatgt cccacttcct cgaccgcctg accttcttca cccgcgacaa
     6241 ggagtccttc tccggcggcc acggcgtggt caccgacgag gaccgcagct gggaggacgc
     6301 ctaccgcaag cgctggcagc acgacaagat cgtgcgctcc acgcacggca ccaactgcac
     6361 cgggtcgtgc agttggaaga tctacgtcaa gggcggcgtg gtgacctggg agacgcagca
     6421 gaccgactac ccgcgcacgc gccccgacat gcccaatcac gagccgcgcg gctgctcgcg
     6481 cggggcgtcg tactcctggt acctgtacag cgccaaccgc ttgaagtacc cgatggtgcg
     6541 cgggcgcctg gtgcgcctgt ggcgcgaggc gcgcaagcac ctggggccgg tcgaggcctg
     6601 ggcttcgatc gtgcaggatc cggtcaaggc caaatcctac aagtcggtgc gtggcatggg
     6661 cggattcgtg cgctcgacct gggacgaagt gcaggagatc gtcgccgccg ccaacgtgta
     6721 caccatcaag cagcatggcc ccgaccgggt gatcggcttc tcgccgatcc cggcgatgtc
     6781 gatggtgtcc tatgccgccg gcagccgtta cctctcgctg atcggcggcg tgtgcatgag
     6841 cttctacgac tggtactgcg atctgccgcc gtcgagcccg cagacctggg gcgagcagac
     6901 cgacgtgccg gaatcggccg actggtacaa ctcgaccttc ctcatcgcct ggggctcgaa
     6961 cgtaccgcag acgcgcaccc cggatgcgca tttcttcacc gaggtgcgct acaagggcgc
     7021 gaaagtggtg gccgtgaccc cggattacgc ggaggtctcc aagctcgccg acctgtggct
     7081 gcatcccaag cagggcaccg acgcggcgct ggcgatggcg atggggcacg tgatcctgaa
     7141 ggagttccac gtcgatcggc aggcgcccta cttcgcggac tacgtgcgcc gctacaccga
     7201 catgccgctg ctggtgcgcc tcaacaagcg tggcgagcgc tatatcgccg accgcttcct
     7261 gcgcgcgagc gacttcggcg acaagctcgg gcaggccaac aacccggaat ggaagacgct
     7321 ggcgctggac gaggcggccg gcgcgggagc gcccagcgtg gtggtgccca acggctcgat
     7381 cggtttccgc tggaaccaga agccgggcga ggatcagggc aagtggaacc tggaggacag
     7441 ggacgcgcgc agcgccgccc cggtcaagct gcgcctctct ctcatcgatg cgcgcgacac
     7501 ggtggcgccg gtggctttcc cctacttcgg cggcatcgcg cacgagcact tccggcacaa
     7561 cgaccagggc agcgaagtgc tggtgcgcaa cgtcccggtg cgcaagctgc agctcgctga
     7621 cggcgagacc ctggtcgcca ccgtgttcga cctgctgctc gccaactacg gcatcgaccg
     7681 cggcctgggc ggtgagcggc tcgcgcgctc gtacgacgac gacgtgccct acacgcccag
     7741 gtggcaggag gcgatcaccg gggtcaaggc gcaggacgtg atcacggtgg cgcggcagtt
     7801 cgccgacaac gccgagaaaa cgcagggcaa atccatgatc atcatcggcg cggcgatgaa
     7861 ccactggtac cactgcgaca tgaactaccg cggcatcatc aacctgctga tgatgtgcgg
     7921 ctgtgtgggc cagtcgggcg gcggctgggc gcactacgtc ggccaggaga agctgcgccc
     7981 gcagaccggc tggaccgcgc tcgcgttcgc gctcgactgg caccggccgc cgcggcagca
     8041 gaactcgacc tcgttcttct atgcgcatac cgaccagtgg cgctacgaga agctgcaggt
     8101 ccatgagatc ctgtcgccgc tcgccgatcc caacgacttc accggcagcc tggtcgactt
     8161 caacgtgcgc gccgagcgca tgggctggct gccgtccgcg ccgcagctgc agaccaatcc
     8221 gctgcgggtg tgcagggacg ccgcccaggc cgggctggac cccagggact acgcggtcca
     8281 ggcgctgaag tccggcagcc tgaagctcgc ctgtgaagat ccggacgacc ccgccaactt
     8341 tccgcgcaac ctgttcgtgt ggcgctccaa cctgctgggt tcctccggca aggggcacga
     8401 gtatttcctc aagcacctgc tcggcacgcg gcacggcgtg cagggcaagg acctcggcgc
     8461 cgaaggcggc agcaagccgg aggaagtcgc gtggcacgac gacgcgccgg agggcaagct
     8521 cgacctgctg gtcacgctcg acttccgcat gtccaccacc tgcctgtact cggacgtggt
     8581 gctcccgacc gccacctggt acgagaagaa cgacctcaac acctccgaca tgcacccctt
     8641 catccacccg ctgtcgtgcg cggtggaccc ggtgtgggaa gcgcgctcgg actgggaaat
     8701 ctacaagggc atcgccaaga agttctccga ggtcgccgtg ggacacctgg gggtggagaa
     8761 ggagctggtg ctcacgcccc tgatgcacga tacgcccgcc gagctcgcgc aggcgctgga
     8821 cgtgaaggac tggaagcgcg gcgagtgcga tttgatcccc ggcaagaccg caccgcagat
     8881 gacggtgatc gagcgcgact atcccaacac ctggcgcaag ttcaccgcgt tggggccgct
     8941 gatgaccaag gccggcaacg gcggcaaagg catcgcctgg aacaccgagc acgaggtgcg
     9001 caatctcgcc gcgctcaacc aggtcgtgat cgaggacggc atcaccaaag gcatgccgcg
     9061 catcgacacc gacatcgatg cctgcgaggt gatcctgatg ctggcgcccg agaccaacgg
     9121 cgaggtggcg gtcaaagcct gggaagcgct gtccaaggtc accggccgcg agcacgagca
     9181 cctcgccatc ccgcgcgaag acgacaagat tcgcttccgc gacgtgcagg cgcagccgcg
     9241 caagatcatc tcctcgccca cctggtcggg gctcgagtcc gagaaggtaa gctacaacgc
     9301 cggctacacc aacgtgcacg agctgattcc ctggcgcacc ttgaccggcc ggcaacagtt
     9361 ctaccaggac cacaagtgga tgctcgggtt cggcgagggg ctgtgcgcct accgcccgcc
     9421 ggtcgacaca cgcagcgtcg cgccgctgct gggcaagcgc ggcaacggca accccgaggt
     9481 gctgctcaac ttcatcacgc cgcaccagaa gtggggcatc cacagcacct acaccgacaa
     9541 tctgctcatg ctgacgctgt cgcgcggcgg cccgatcgtc tgggtgtcgg aagtcgacgc
     9601 gaggaagatg aacgtggagg acaacgactg gatcgagctg tacaacatca acggcgccat
     9661 caccgcgcgg gcggtggtgt cgcagcgcgt agcgcagggg atgtgcctga tgtaccacgc
     9721 ccaggagaag atcctgaacg tgcccggctc cgagatcacc ggcacgcgcg ggggcatcca
     9781 caactcggtg acgcgcaccg tgaccaagcc cacgcacatg atcggcggct acgcgcaact
     9841 cgcctacggc ttcaactact acggcacgat cggcaccaac cgcgacgagt tcgtgatcgt
     9901 gcgcaagatg cagaccatcg actggatgga tgagccagcc gagaacgtcc tctaggagca
     9961 agagatgaaa atacgcgccc agatcggcat ggtcctgaac ctggacaagt gcatcggctg
    10021 ccacacctgc tcggtcacct gcaagaacgt gtggacctcg cgcgaaggca tggagtacgc
    10081 atggttcaac aacgtcgaga ccaagcccgg catcggctac ccgaaggagt gggagaacca
    10141 gcagcgctgg agcggaggct gggtgcgcaa ggcgagcggc aggatcgagc ccaggatcgg
    10201 cggcaagtgg caggtgctga tgaagatctt cgccaacccg cacctgccgg agatcgacga
    10261 ctactacgag ccgttcacct tcgactacga gcacctgcac accgcccccg agatgaaagc
    10321 cccaccggtg gcacgcccgc gcagcctggt gagcggcaag ccgctggaga agatcgaatg
    10381 gggccccaac tgggaggaga tcctcggcgg ggagttcgcc aagcgctcgc aggacagcaa
    10441 cttcgaggcg gtgcagaagg acatctacgg gcagttcgag aacaccttca tgatgtacct
    10501 gccgcgcctg tgcgagcact gcctcaatcc ggcttgcgtg gcggcctgcc cctccggctc
    10561 gatctacaag cgcgaggagg acggcatcgt cctggtcgat caggacaagt gccgcggctg
    10621 gcggatgtgc gtctccgcct gcccctacaa gaagatctat tacaactggc aatccggcaa
    10681 ggccgagaaa tgcaccttct gctatccgcg catcgaggtc ggccagccga ccgtctgctc
    10741 ggagacctgc gtcggccgca tccgttacct cggcgtgatg ctctatgacg ccgaccggat
    10801 tgaagaggcg gcgagcgcgg agcacgagca ggacctctac gaggcgcagc tcgcgatctt
    10861 cctcgacccg agggatcggg aggtgatcga gcgtgcccgc gccgacggcg tgcccgaggc
    10921 ttggatcgag gccgcgcagg cctcgcccgt ccatcgcatg gcgatcgagt ggaagattgc
    10981 cttcccgctg catcctgaat accggacctt gccgatggtc tggtacgtgc cgccgctctc
    11041 gccggtgcag ggcgcggccg aggccggtca tgtcggcttc aaggacgagc ttcccgacat
    11101 cgacgccctg cgaatcccgg tccgctatct ggccaacatg ctcacggcgg gcgacacgga
    11161 gccggtttct ctggcgctca agcgaatgtt cgcgatgcgc atctatcagc gtggccggca
    11221 tgtcgacggc accctccaca cggatgtgct gcgccaggtt gggctgactg ccaatcaggt
    11281 agaggagatg taccgtctgc tcgccatcgc caactacgaa gaccgcttcg tgatcccgac
    11341 cacgcaccgc gagtacgccg aggacgcctt cgatctaagg ggctcctgcg ggttcagctt
    11401 cggcaatggc tgctcgcacg ggatgagtga gccgaagctg ttcggcgcgc cggcgccgcg
    11461 caaggagatg gcgtgatggc gacggtctcc tacaaggcgc tgggggcgct gctggactat
    11521 cccgaggccg agctgatcga ggtgttgccg gagatcggcg ccattctcga cgaagagccc
    11581 gccttctcgc cggagactcg g
//
LOCUS       DASPGE010000161         2950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_45043, whole genome shotgun sequence.
ACCESSION   DASPGE010000161 DASPGE010000000
VERSION     DASPGE010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45043"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..1262
                     /locus_tag="VD791_05100"
     CDS             9..1262
                     /locus_tag="VD791_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXV07383.1"
                     /translation="MCLHAGQIPDPTTGARAVPIYQTTSYVFDSADHAASLFNLQTFG
                     NVYSRISNPTVAVFEERVAALEGGRAALAAASGQAAQMTALLTLLDQGDQIVSASTLY
                     GGTYSQFEVTFRKFGIDTRFVDPEDPQNFKKALTPRTKVIYAETAGNPRINVLDIQAV
                     AAIAKDAGIPLVIDNTFASPYLCQPFQWGADIVLHSATKYIGGHGTTMGGVLVESGRF
                     PWDNGKFPGMTEPSRGYHGVRFFETFGDFGYSMKCRMETLRTLGPAMSPFSAWILLQG
                     LETLHVRMDRHVSNALKVAEYLASHPQVSWVSYPGLRSSPYYELARKYMPKGAGAIMT
                     FGIKGGQAAGVKFIEGVEFLSHLANVGDAKTLVIHPASTTHRQLSEEEQIQAGVTPDM
                     VRLSVGIEDIDDILWDLDQALAKAR"
     gene            1275..2444
                     /locus_tag="VD791_05105"
     CDS             1275..2444
                     /locus_tag="VD791_05105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV07384.1"
                     /translation="MSIWPSLWITLAIQVLASLVLYVPPVLAPAAQGDVGVPASFVGI
                     VTAAVFATATFGALLSGAPIARRGALRVSQVSLVVCGAGLAVMASANAWLIVLGALVI
                     GLGYGTVTPASSVILNDRAPPGLRAFIFSLKQTGVPVGGALAGALLPGLMALVGWRWA
                     TLGPAGACVLLALALEPLRPTLESPRDEQPPHPHIPMLRALRLVMHEPRLRELALASF
                     TYSGIQNCLGSFLVVYLHERVGFGLGAAGLALSAAMAAGIAGRLLWGVAADRYANPRR
                     LLGGLGVAMSAAAFLTATFSPDWPVAGVLAVALVFGLTAVGWNGVYISEAARIAARIH
                     AGAATGAAFAFTYAGVVSSPLLFLGVVQASGSYGLAYAVVAALTLWRGAILIGKA"
     gene            2606..>2950
                     /locus_tag="VD791_05110"
     CDS             2606..>2950
                     /locus_tag="VD791_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXV07385.1"
                     /translation="MAVTNSPGDSKPTGEAKPCRGRPRSEHSHEAILDAVLELLEAEG
                     YGALTIEGVARQAGVGKQTIYRWWKCKAELVLEAYANHAASKIPVPDRGTVRGDLEAF
                     LGAGFKRLTDISG"
BASE COUNT          423 a         1031 c         1013 g          483 t
ORIGIN      
        1 tcggcacgct gtgcctgcac gccggacaga tccccgatcc gaccaccggc gcgcgcgctg
       61 tgccgatcta ccagaccacg tcctacgtgt tcgactcggc cgaccacgcg gccagcctgt
      121 tcaatctgca gaccttcggc aacgtctatt cgcgcatctc caatcccacg gtggcggtgt
      181 tcgaggagcg cgtggccgcg ctcgagggcg ggcgcgcggc gctggccgcc gcctcgggcc
      241 aggcggcgca gatgaccgcg ctgctcacgc tgctcgatca aggcgaccag atcgtctcgg
      301 cgagcacgct ctacggcggc acctactcgc agttcgaggt caccttccgc aagttcggca
      361 tcgacaccag gttcgtcgac cccgaggacc cgcagaattt caagaaggcg ctcacgccgc
      421 gcaccaaggt gatctacgcc gaaaccgccg gcaatccgcg catcaacgtc ctcgacatcc
      481 aggcggtggc cgccatcgcc aaggatgccg gcatcccgct ggtgatcgac aataccttcg
      541 ccagccccta cctgtgccag ccgttccagt ggggggcgga catcgtgctg cactcggcca
      601 ccaaatacat cggcggccac ggcaccacca tgggcggggt gctggtcgaa tccggcaggt
      661 tcccctggga caacggcaag ttcccgggca tgaccgagcc ctcgcgcggc taccacgggg
      721 tgcgcttctt cgagaccttc ggcgacttcg gctacagcat gaagtgccgc atggagacgc
      781 tgcgcaccct ggggccggcg atgtcgccgt tcagcgcgtg gatcctgctg caagggctgg
      841 aaacgctgca cgtgcgcatg gaccggcacg tgtccaacgc gctcaaggtg gccgagtatc
      901 tcgcctcgca tccgcaggtg agctgggtga gctatccggg cctgcgcagc agcccgtact
      961 acgagctggc ccgcaagtac atgccgaagg gcgcgggggc gatcatgacc ttcggcatca
     1021 agggcggaca ggccgccggc gtcaagttca tcgagggcgt cgagttcctc agccatctgg
     1081 ccaacgtcgg agacgccaag acgctggtca tccacccggc ctccaccacg caccgccagc
     1141 tctccgagga ggagcagatc caggccggcg tcacgccgga catggtgcgc ctgtcggtcg
     1201 gcatcgagga catcgacgat atcctgtggg acctggatca ggcgctggcg aaagctcgct
     1261 gagccggctc cgcagtgagc atctggccct cgctgtggat cacgctcgcg atccaggtgc
     1321 tggcctcgct ggtgctttac gtgccgccgg tgctggcgcc ggcggcgcaa ggggacgtcg
     1381 gcgtgccggc gagcttcgtc ggcatcgtca ccgccgcggt gttcgccacg gccaccttcg
     1441 gcgccctgct gtcgggcgct cccatcgcgc ggcgtggcgc cctgcgcgtc agccaggtga
     1501 gcctggtcgt gtgcggcgcc ggactggcgg tgatggcgag cgccaacgcc tggctgatcg
     1561 tgctcggggc gctggtgatc ggcctggggt acgggaccgt gacgccagcg agctccgtca
     1621 tcctgaacga tcgcgccccg ccggggctgc gcgcgttcat cttctcgctc aagcagaccg
     1681 gcgtgccggt cggtggagcg ctggccggcg ccctgctgcc ggggttgatg gcgctggtgg
     1741 gctggcgctg ggcgacgctt ggccccgccg gcgcctgcgt gctgctggcg ctggcgctcg
     1801 agcccctgcg cccgacgctc gaaagcccgc gcgacgagca gccgccgcac ccccacatcc
     1861 cgatgctccg agcgctgcgt ctggtcatgc acgagccgcg gctgcgcgag ctggcgctcg
     1921 cttcgttcac ctactcgggg attcagaatt gcctggggtc gttcctggtg gtgtacctgc
     1981 acgagcgcgt cgggttcggg ctcggcgccg ccggtctggc gctctcggca gcgatggcgg
     2041 ccgggatcgc cgggcgcctg ctgtggggtg tggcggccga ccgctacgca aacccgcggc
     2101 gcctgctcgg cgggctcggg gtcgcgatgt cggccgccgc cttcctcacc gcgacgttca
     2161 gcccggactg gcccgtggcg ggcgtgctgg ccgtggcgct cgtgttcggt ctgaccgcgg
     2221 tgggttggaa cggcgtctac atctccgagg cggcgcgcat cgctgcgcgc atccatgccg
     2281 gggcggcgac cggggcggcg ttcgcgttca cttatgccgg cgtcgtgtct tcgccgctgc
     2341 tcttcctcgg tgtggttcaa gccagcggca gctacggcct ggcctacgcg gtggtcgccg
     2401 cgctgacgct gtggcgcggc gcgattctga tcggtaaggc gtgatcggta gagcattgtc
     2461 gaaactgcgt gatcgaaagg cgtgatcgaa cagcgtatcg aaccagcttg gtaacctggc
     2521 tacgcttctc gatcacgcag taccgatcac agtgtggtac cctcccgcct ccggatcgca
     2581 cgcggcctcg gccgctccac cagcaatggc cgtcaccaat tcccctggcg attccaagcc
     2641 cactggcgaa gccaagccct gccgcgggcg cccgcgcagc gagcactcgc acgaggcgat
     2701 cctggacgcg gtgctcgagc tgctcgaagc cgaaggctat ggcgcgctca ccatcgaagg
     2761 ggtggcgcgg caggccggcg tgggcaagca gaccatctat cgctggtgga aatgcaaggc
     2821 cgagctggtg ctcgaggcct acgccaacca tgccgccagt aagatccccg tgcccgaccg
     2881 cggcacggtg cgcggcgacc tggaagcctt cctcggcgcc ggcttcaagc gcctgaccga
     2941 catctccggg
//
LOCUS       DASPGE010000162         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_45524, whole genome shotgun sequence.
ACCESSION   DASPGE010000162 DASPGE010000000
VERSION     DASPGE010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45524"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VD791_05115"
     CDS             <1..346
                     /locus_tag="VD791_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HXV07386.1"
                     /translation="LGKLVTEMVHDALHAFARLEADEAFEVARRDRKVDEEYEAIQRQ
                     NITFMMEDPRSIRRALEIMWVVRALERIGDHAKNICEYTVYMVHGKDIRHLSLDDAER
                     QIRDFARDRKGA"
     gene            346..831
                     /locus_tag="VD791_05120"
     CDS             346..831
                     /locus_tag="VD791_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HXV07387.1"
                     /translation="MPSRRAFNGLGAGASAAALAIAFWSQYGLGLVPCHLCIFQRVTV
                     AVLAAGFLSAAIISRPGWRGALAAVLVAVPGVATIATAGRHVWIQMQPEGSVPACGAD
                     LDFMLDLFPVTEVILKVFKAGGECAKVDWTLLGLSMPGWVLVFAVVLTAGGIWNNLRR
                     A"
     gene            complement(844..1939)
                     /gene="dapE"
                     /locus_tag="VD791_05125"
                     /pseudo
     CDS             complement(844..1939)
                     /gene="dapE"
                     /locus_tag="VD791_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298058.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
     gene            complement(1949..>2039)
                     /gene="dapD"
                     /locus_tag="VD791_05130"
     CDS             complement(1949..>2039)
                     /gene="dapD"
                     /locus_tag="VD791_05130"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812536.1"
                     /note="catalyzes the formation of
                     N-succinyl-2-amino-6-ketopimelate from succinyl-CoA and
                     tetrahydrodipicolinate in the lysine biosynthetic pathway;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HXV07388.1"
                     /translation="SHSLYCAVIVKKVDAQTRAKTSINELLRE"
BASE COUNT          286 a          712 c          707 g          334 t
ORIGIN      
        1 cctcggcaag ctggtcaccg agatggtgca cgacgcgctg cacgcttttg cacgcctgga
       61 ggcggacgag gccttcgagg tggcgcgccg ggaccgcaag gtggatgagg aatacgaggc
      121 catccagcgc cagaacatca ccttcatgat ggaagacccg cgctcgatcc ggcgcgcgct
      181 cgagatcatg tgggtggtgc gcgcgctgga gcgcatcggc gaccacgcca agaacatctg
      241 cgagtacacg gtctacatgg tccacggcaa ggacatccgc cacctgtcgc ttgacgacgc
      301 ggagcggcag atccgggact ttgcgcgcga ccgcaagggc gcctgatgcc gtcgcgccgc
      361 gcgttcaacg ggctcggcgc cggggccagc gcggccgcgc tcgccatcgc tttctggagc
      421 cagtacggcc tcgggctcgt gccctgccac ctgtgcatct tccagcgcgt gacggtcgcg
      481 gtgctggcgg cgggcttcct gagcgccgcg atcatctcgc ggccgggctg gcgcggggcg
      541 ctggcagcgg tactcgtcgc agtccccggt gtcgcgacca tcgcgaccgc cgggcgtcac
      601 gtgtggatcc agatgcagcc cgagggctcg gtgcccgcct gcggggccga tctcgacttc
      661 atgctggacc tgttcccggt gaccgaggtg atcctcaaag tcttcaaggc cggcggtgag
      721 tgcgccaagg tggactggac tttgctgggg ctctcgatgc ccggctgggt gctggtgttc
      781 gccgtggtgc tgaccgccgg cggcatctgg aacaacctgc gccgcgcctg atgcggcgcc
      841 ggttcaggcc gggggcagca atctttccag cgtgcccacc agggcacggt gcagcaggtc
      901 gatttcctcc acgtcgcagt tctcgtcgag cttgtggatg gattcgttgc gcacgcccag
      961 ttccacgatc tccgcgccca tgggggcgat gaaccggccg tccgaggtcc cgcccccggt
     1021 cgtcgcctgc gggcgctgcc cggtcagttg ctcgatcacg gcggttgccg cggctgccag
     1081 ccgctcaggc ggcgagtaga agggtagccc ggcctcgtac cagtcgatcg catagcgcag
     1141 gccgtggcgg tcgaggatgc cctgcaccgc ttgcttgagt ccgtccagcg tctgcgccgg
     1201 cgaccagcgc aggttgaacc gcactaccag ctcgcccggt atcacgttcg gcgccccggt
     1261 gcccgcgccc aggttactga cctggaagct ggtcgccttg aaatggccgt ggccctcgtc
     1321 ccagcggcga gcgacgagtt ccgccagcgc cggcgcgaag cggtggaccg gattgtcggc
     1381 ccgctcggga taggcgacgt gcccctgcac gccgtgcacc gtgagccgcg ccgagagcga
     1441 gccccggcgg ccgatgcgga tgttgtcgcc gaagcggtcg aggctcgacg gctccgcgat
     1501 caggcagagg tcgatcccct ggccctgcgc ctgcagccac tcgacgacgc ggcgcgtgcc
     1561 gtccaccgac ctgccttcct cgtcgctggt gaccaggaac gcgatcgcgc cggcatgcgc
     1621 gggccgccgg ccgacgaatt cctcggtcgc cgtcaccatc gcggccagtc cactcttcat
     1681 gtccgcagtg ccgcggccgt acagccgtcc gtctcggatc acgggctcga acgggtcgga
     1741 gctccagcgc tctgccgggc ccgccccggc gcagcgccca gaagttctcc acttcgccgt
     1801 accgcaggcg ctcgaccttg aagccgagcg gcgcaaggcg cgcgatcagc aggtcctggc
     1861 agccggcgtc gtcgggcgta atcgaacggc gcgccaccag cgcacgcgtc aactccaccg
     1921 tgggtgaatg ctggctcacg ggtggcgcct attcgcgcag cagttcattg atggacgtct
     1981 tcgcgcgcgt ctgcgcgtcc accttcttga cgatgactgc gcagtagagc gaatggctg
//
LOCUS       DASPGE010000163         5016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1450017, whole genome shotgun sequence.
ACCESSION   DASPGE010000163 DASPGE010000000
VERSION     DASPGE010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450017"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VD791_05135"
     CDS             <1..714
                     /locus_tag="VD791_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXV07389.1"
                     /translation="LAQHADMIFCLVRTASTGKKQEGISFLLIDMRSRGVTVRPIITI
                     EGEHEVNEVWFEDVEVPAENLVGEEGRGWTYAKFLLSHERSGIAAVGASKRELRMLKH
                     IASQEVSGGRPLIEDARFRDRIAQVEIDLMALEITNLRVLAADREGRAPGPEASILKI
                     KGTDIQQALTELMAEAVGPYGLPHLPEAWGDHWLGERVGPDYAAPLAARYFNYRKTSI
                     YGGSNEIQRNIVAQHILGL"
     gene            723..1913
                     /locus_tag="VD791_05140"
     CDS             723..1913
                     /locus_tag="VD791_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXV07390.1"
                     /translation="MDFNYSEEQLLLKDAVEKFLDKNYTFEQRRQIIASRDGISTTAW
                     GGFASLGLLGVPIPQEFGGFGGGGVDVMVVMEALGRALVVEPYLPTVVLAGSAITLGG
                     SAAQRQILLPRIGDGSMKFAFAHTEPGARWELNRVHTCARQEGGMWVIDGQKSVVLHG
                     GVADLLIVTARTSGAPDSTHGITLFFVNPAEQGVMGREYPTYDGMRAAEVTFDNVTIS
                     PESMIGVLDNGYPLLDAVIDRGIAALCAEAVGCMSALHGATLEYLKTRQQFGVPIGRF
                     QALQHRAVDMLIHCEQARSMALLAAVKVDSANAGERRRALSAAKEFIGRAGRFVGQQA
                     IQLHGGMGMTDELNVGHYFKRLTAIDVTFGNGDLHRERFWQAGEAPAEEPVRKPAKRW
                     KQLV"
     gene            2276..3058
                     /locus_tag="VD791_05145"
     CDS             2276..3058
                     /locus_tag="VD791_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV07391.1"
                     /translation="MTQGLLAGDTTLVTGAASGIGRGIAAALAREGARVVLADVEAVR
                     GEEVAEAIRASGGDARFLLADLAAPGAAAKLLASTVEALGSLSILVHSASPRRLEADT
                     VAAVSDETWDRMLAVNLRAGFQLGRDAGKHMRERGIKGRMLYLTSLHADSPRNLPHYS
                     AAKAGETMVMKELARAFGPHGIRVNAIAPGAIPGGGFDAKRAGIERLTRCTSLGRLGT
                     PEDIAGMALALLCDRFSAYVTGTTVVVDGGLALHNWIEPPAS"
     gene            3101..3562
                     /locus_tag="VD791_05150"
     CDS             3101..3562
                     /locus_tag="VD791_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXV07392.1"
                     /translation="MAVKYYWEDFKPGDTFPLGERVMDRDEMLAFARQFDPQPFHVDE
                     AAAKRSMYGGLIASGWHTVALVMRMMCDSYLNQSASLGAPGVDNVRWLKPVRPGDTIR
                     AQRTVLESRASQSRPEMGLVKTRWEVFNQSGELVMTMEGTGMFQRRTPGAR"
     gene            3653..4240
                     /locus_tag="VD791_05155"
     CDS             3653..4240
                     /locus_tag="VD791_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HXV07393.1"
                     /translation="MNPTRWLISATILAATVCSGALAFSAEDDAPKDRGWYIGGGIGA
                     ADDHDFDETEAGGKFFGGYRVGRYFAAEAAAVYLGNYGRGTLGIGDGGEFDKYSFTAQ
                     GVGILPVGRRAELLVKAGLSYWSVDVVETCTVSGGQQVCVDDGTLDEGVDAVYGVGFQ
                     YHFSDRWGGRIEWERYNDVGEDDLDFLSISVLFRF"
     gene            4291..>5016
                     /locus_tag="VD791_05160"
     CDS             4291..>5016
                     /locus_tag="VD791_05160"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HXV07394.1"
                     /translation="MLVRLNHKIHVLFRRQDLDMVKLDGKVAVVLDVLFATSTMISAL
                     ADGAAEVIPTLNEDHAREQAQTLREGSYVLAGELFAETIAGFAPPTPMALMAHELRGK
                     TVVYATTNGTVALKESAAAAHVYAGALLNARAVVEHIARHHGDRTVLIVCSGSMGNPN
                     LEDMYGAGYFVDLLTEELGEGRDFSDAAWAARALYRSERAESLLLRSRVGQMMRERGL
                     TEEVKFAARLSVMDVVPKLVDGRL"
BASE COUNT          834 a         1627 c         1792 g          763 t
ORIGIN      
        1 ctggcgcagc acgcggacat gatcttctgc ctggtgcgca ccgccagcac cggcaagaag
       61 caggagggca tctcctttct gctgatcgac atgcgctcgc gcggggtgac ggtgcggccg
      121 atcatcacca tcgaaggcga gcacgaggtc aacgaagtct ggttcgagga cgtcgaggtt
      181 ccggccgaga acctggtggg cgaggagggc cgcggctgga cctacgccaa gttcctgctg
      241 tcgcacgagc gctccgggat cgcggccgtg ggcgcctcca agcgcgagct gcgcatgctc
      301 aagcacatcg cctcgcagga ggtgagcggt ggtcgcccgc tgatcgagga tgcgcgcttc
      361 cgcgaccgga tcgcacaggt ggagatcgac ctgatggcgc tggagatcac caacctgcgc
      421 gtgctcgccg ccgatcgcga gggccgcgcg ccggggcccg aggcctcgat cctcaagatc
      481 aagggcaccg acatccagca ggcgctgacc gagctgatgg ccgaggcggt cggcccctac
      541 ggcctgccgc acctgccgga ggcctggggc gaccactggc tgggcgagcg cgtgggcccc
      601 gactacgccg cgccgctggc ggcgcgctac ttcaactacc gcaagaccag catctacggc
      661 ggatccaacg agatccagcg caacatcgtc gcgcagcaca tcctgggctt gtgaggcgag
      721 tcatggattt caactacagc gaagagcaac tgctgctgaa ggacgcggtc gagaagttcc
      781 tcgacaagaa ctacaccttc gagcagcgca ggcagatcat cgcctcgcgc gacggcatca
      841 gcaccaccgc ctggggaggc ttcgcctcgc tcggcctgct cggcgtgccg attccgcagg
      901 agttcggcgg cttcggcggt gggggcgtcg acgtgatggt ggtgatggag gcgctcgggc
      961 gcgccctggt ggtggaacct tatcttccga ccgtggtgct ggcgggcagc gcgatcacgc
     1021 tgggcggcag cgcggcccag cgccagatcc tgctgccgcg catcggcgac gggtcgatga
     1081 agttcgcctt cgcgcacacc gagccgggcg ctcgctggga gttgaaccgc gtgcatacct
     1141 gcgcgcgcca ggagggcggc atgtgggtga tcgacgggca gaaatcggtg gtgctgcacg
     1201 gcggcgtcgc cgatctgctg atcgtcaccg cgcgcaccag cggcgcgccg gacagcacgc
     1261 acggcatcac gctgttcttc gtgaacccgg ccgagcaggg cgtgatgggc cgagagtacc
     1321 ccacctacga cggcatgcgc gcggccgaag tcaccttcga caacgtcacc atcagccccg
     1381 agtcgatgat cggcgtgctc gacaacggct acccgctgct cgacgcggtg atcgatcgcg
     1441 gcatcgccgc gctgtgcgcc gaggcggtgg gctgcatgag cgcgctgcac ggcgcgaccc
     1501 tcgagtacct gaagacgcgc cagcagttcg gcgtgccgat cgggcgtttc caggccctgc
     1561 agcaccgcgc ggtggacatg ctgatccact gcgagcaggc gcgctcgatg gcgctgctcg
     1621 cggcggtgaa ggtggattcg gcgaacgcgg gcgagcgccg gcgcgcgctg tccgcggcca
     1681 aggagttcat cggtcgcgcc gggcgcttcg tggggcagca ggcgatccag ctgcacggcg
     1741 gcatgggcat gaccgacgag ctcaacgtcg ggcactactt caagcgcctg accgccatcg
     1801 acgtcacctt cggcaacggc gatctgcacc gcgagcgctt ctggcaagcg ggggaggcgc
     1861 cggccgaaga gccggtgcgc aaacccgcga agagatggaa gcagctggtg tgaagcggcg
     1921 cctgagcgcg tggcttcggt gagcgcgctc ttgtgcgttc tacgacagat gccagcggga
     1981 gcggcgcagt ctccagcgtc cttggggtga ttcgcgccgc tcgcgccgcc acaggcgcca
     2041 cgccgaccac aggcaccaga ccgagatccc cagcagggcg atcacgacac tccaagcaac
     2101 ggtctgcagc attcgtcggc aatcggctgt gttacggcaa cggatgcaaa tctagtcgca
     2161 ccgatcgagc gcggtcaacg aataccaacc gagttgacat tccgtcgctc gcgccgttca
     2221 cttttgtcgc ttgacggaga cagttgtggc ctcaccacgc gaacggagcg aaaccatgac
     2281 acagggattg ctggccgggg acaccacgct cgtcaccggc gcggcatcgg gcatcggacg
     2341 cggaatcgcg gcggcgctgg cgcgcgaggg cgcgcgcgtc gtgctcgccg acgtcgaggc
     2401 ggtgcgcggc gaggaggtgg cggaagcgat ccgcgcttcg ggaggcgacg cgcgcttcct
     2461 ccttgccgat ctcgccgcac ccggcgccgc cgccaagctg ctcgcatcga cagtggaagc
     2521 gcttggctcg ctgtcgatcc tggtccacag cgccagccca cgccggctcg aggccgacac
     2581 cgtggcggcg gtgagcgatg aaacctggga ccgcatgctg gcggtgaacc tgcgcgcggg
     2641 cttccagctc gggcgcgacg ccggcaagca catgcgcgag cggggcatca agggacgcat
     2701 gctgtacctg acgtcgctgc acgcggactc gccccgcaat ctgccgcact acagcgcggc
     2761 caaggcggga gaaaccatgg tgatgaagga actcgcgcgc gcattcggcc cgcacggcat
     2821 ccgcgtcaac gcgatcgccc caggcgcgat tcccggcggc gggttcgatg ccaagcgcgc
     2881 cggcatcgag cggctcacgc gctgcaccag cctggggcgg ctgggcacgc cggaagatat
     2941 cgccggcatg gcgctggcgt tgctgtgcga ccgcttctca gcctacgtga ccggcaccac
     3001 cgtcgtggtg gacggcggtc tcgcgctcca caactggatc gagccgccgg cgtcctagag
     3061 cgtcgcggcg ccgcacgtcg ttcacggacg aggggttcag atggctgtca agtactattg
     3121 ggaagacttc aagcccggcg acaccttccc gctgggagag cgggtgatgg atcgcgacga
     3181 gatgctggcg ttcgcccggc agttcgaccc gcagcctttc cacgtcgacg aagcggcggc
     3241 gaagcgctcg atgtacggtg gcctgatcgc cagcggctgg cacaccgtgg cgctcgtcat
     3301 gcgcatgatg tgcgattcgt acttgaacca gtcggccagt ctgggcgcgc cgggcgtgga
     3361 caacgtgcgc tggctgaagc cggtgcggcc gggcgacacc atccgcgcgc agcgcaccgt
     3421 gctcgagtcg cgcgcctcgc agagccggcc ggagatgggg ctggtgaaga cgcgctggga
     3481 agtgttcaac cagagcggcg agctggtgat gaccatggaa ggcaccggca tgttccagcg
     3541 gcgcactccg ggggcgcgct agatgccggt acgcgagcgg gtgatcgcct gccttgagcc
     3601 cttccgtggc acgtgatacg gttgaatctt ctatttgtcg aagggggcga ccatgaaccc
     3661 gacgcgatgg ctgatcagtg cgaccatcct cgccgccacg gtgtgctcgg gggcgttggc
     3721 gttcagcgcc gaggacgacg cgccaaagga caggggctgg tacatcggtg gtggcatcgg
     3781 cgcagcggac gaccacgact tcgatgagac cgaggccggg gggaaattct tcggcggcta
     3841 ccgcgtcggc agatactttg ccgccgaagc ggcagccgtc tacctgggta actacggacg
     3901 cggcacgctg ggcatcggtg acggcggtga gttcgacaaa tacagcttca ccgcgcaggg
     3961 cgtaggcatc ctgcccgtcg gtcggcgcgc agagctcctg gtcaaggcgg gtctgtccta
     4021 ctggagcgtg gatgtcgtcg agacctgcac ggtgtcgggc gggcaacagg tatgcgtgga
     4081 cgacggcacg ctcgacgaag gcgtggatgc ggtctacgga gtcggcttcc agtatcattt
     4141 cagcgatcgc tggggcggcc gcatcgagtg ggaacggtat aacgacgtcg gcgaagacga
     4201 cctggacttc ttatcgatca gcgtgctgtt ccgcttctga ggatatccaa ggccgggatg
     4261 tgccagcgcc gcaccgcggg ggcgcgctag atgctggtgc gcctgaacca caagatccac
     4321 gtcctgttcc gccggcagga cctggacatg gtcaagctcg acggcaaggt ggcggtggtg
     4381 ctggacgtgc tgttcgccac ttccaccatg atctcggcgc tggccgacgg cgccgccgag
     4441 gtgattccca cgctcaacga ggaccacgcg cgcgagcagg cgcagacgct gagggagggc
     4501 agctacgtgc tcgccggcga gctgttcgcc gagaccatcg cgggcttcgc accgcccacg
     4561 ccgatggcgc tgatggcgca cgagctgcgc ggcaagaccg tggtgtacgc caccaccaac
     4621 ggcacggtgg cgctgaaaga gtcggctgcc gccgcgcacg tgtatgccgg cgcgctgctc
     4681 aacgcgcgcg ccgtggtgga gcacattgca cgccatcacg gcgaccgcac cgtcctgatc
     4741 gtgtgctcgg ggtcgatggg caacccgaac ctggaggaca tgtacggcgc cggctacttc
     4801 gtcgatctgc tcaccgagga gctgggtgag ggccgcgatt tctccgacgc cgcctgggcc
     4861 gcgcgcgccc tgtaccgcag cgagcgcgcc gagtcgctgc tgctgcgctc gcgggtcgga
     4921 cagatgatgc gggagcgcgg gctgaccgaa gaggtcaagt tcgccgcacg cctgagcgtc
     4981 atggacgtgg tgcccaagct ggtcgacggc cggcta
//
LOCUS       DASPGE010000164         3682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1100002, whole genome shotgun sequence.
ACCESSION   DASPGE010000164 DASPGE010000000
VERSION     DASPGE010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100002"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..93
                     /locus_tag="VD791_05165"
     CDS             <1..93
                     /locus_tag="VD791_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HXV07395.1"
                     /translation="EGVGIDRLVMLLTDSPSIRDVILFPQLRPE"
     gene            104..2068
                     /locus_tag="VD791_05170"
     CDS             104..2068
                     /locus_tag="VD791_05170"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HXV07396.1"
                     /translation="MPSEAPTLLWQPSAERIAQANLTRFIRLVDERWQAGAHDHASLY
                     QWSIREPARFWEAVWDFAGVIGDKGKPPYLLHSERMPGAAWFPRARLNFAENLLRRRD
                     DDAALVFWSEDKVRRKLSFNELYDQVSRTGQALRALGVSPGDRVAGFLPNMPEAMIAM
                     LATASTGAIWSSCSPDFGVQGVLDRFGQIAPKVLFCVDGYHYAGKSHDTLARIGEIAR
                     QLPSLQRVVVVPHLAPAPRIDGIANAETLTRFLAPFTPGDIPFERGPFDRPQFILYSS
                     GTTGVPKCIVHGAGGTLLQHLKEHQLHTDLKRGERLFYFTTCGWMMWNWLASGLATGA
                     TLLLYDGSPFHPGPAVLFDLADAEGMTVFGTSAKYIDALGKVGLRPRDTHRLASLRTM
                     TSTGSPLSPESFEYVYRAIKQDLLLSSISGGTDIVSCFVLGSPLLPVWKGEIQCRGLG
                     LKVEVYDDDGRPVIGEKGELVCSAPFPSMPVDFWNDPDGAKYREAYFARFPGVWCHGD
                     YMELTPRGTAVIYGRSDAVLNPGGVRIGTAEIYRQVEQLPEVVESLVIGQDWERDVRV
                     VLFVRLRDGLTLDQALVDKIKRQIRTNTTPRHVPAKICQVEDIPRTKSGKIVELAVRN
                     VVHGRPVKNREALANPEALALFENLPELQR"
     gene            2313..2537
                     /locus_tag="VD791_05175"
     CDS             2313..2537
                     /locus_tag="VD791_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HXV07397.1"
                     /translation="MDQTRLRIEGMTCPGCVRSVKRVLEAVAGVRGAAVSLEQGEAVV
                     DFDPDRASTADLVHAVEGAGYRAQPVQRGS"
     gene            complement(2539..3351)
                     /locus_tag="VD791_05180"
     CDS             complement(2539..3351)
                     /locus_tag="VD791_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HXV07398.1"
                     /translation="MSKLRSLAGFASALLLVGSVLLPACRSTGDDKYDETRNWSAERL
                     YNEAKAELLSGDYKKSIEYYQKLEARYPYGRYAQQSQLELAYAYYKDVEPVLALAETD
                     RFIKLYPEHPNVDYAYYLKGLINFNEDLGVFGGFANQDMSERDPRAAIESFESFRELV
                     QRFPDSRYVPDAYARMRYLINALANNEVRVGEYYLRRKAYVAAVNRAQYVLTNYPKTP
                     AVEQALIVMVKAYDAMGLAELRDDADRLLKLNYPSTKYKLANRQERKWWQFW"
     gene            3350..>3682
                     /locus_tag="VD791_05185"
     CDS             3350..>3682
                     /locus_tag="VD791_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HXV07399.1"
                     /translation="MSLEKRARDYSASSAAPAPAALGKARHARVPDALAGLRLDQALA
                     RLFPEYSRSRLQTWVKSARVTLDGALARPRDKVRGGERVALRPDADQGAARDLAQDIP
                     LGILFEDGA"
BASE COUNT          594 a         1219 c         1268 g          601 t
ORIGIN      
        1 gaaggcgtgg gcatcgaccg gctggtgatg ctgctcaccg acagcccgag catccgcgac
       61 gtgatcctgt ttccgcagct gcgccccgag tagccggtcg gacatgccca gcgaagcccc
      121 cacgctgctc tggcagccct cggcggagcg catcgcccag gccaacctga cgcgcttcat
      181 ccggctggtc gacgagcgct ggcaggccgg cgcgcacgac catgcgtcgc tgtatcagtg
      241 gtcgatccgc gagccggcga ggttctggga ggcggtgtgg gatttcgccg gcgtgatcgg
      301 cgacaagggc aagccgcctt acctgctcca cagtgagcgc atgcccggcg cggcctggtt
      361 cccgcgcgcc cggctcaact tcgcggagaa cctgctgcgc cggcgcgacg acgatgccgc
      421 gctggtgttc tggagcgaag acaaggtgcg gcgcaagctc agcttcaacg agttgtacga
      481 ccaggtgtcg cgcacggggc aggcgctgcg cgcgctgggg gtgagccccg gcgaccgcgt
      541 ggccggcttc ctgcccaaca tgcccgaggc gatgatcgcc atgctggcca cggccagcac
      601 cggcgcgatc tggtcttcct gctcgccgga cttcggcgtg caaggcgtgc tcgaccgctt
      661 cggccagatc gctcccaagg tgctgttctg cgtggacggc tatcactacg ccggcaagag
      721 ccacgacacg ctggcgcgca tcggcgagat cgcgcggcag cttccgagcc tgcagcgcgt
      781 ggtggtcgtg cctcacctgg cgccggcgcc gcggatcgac ggcatcgcca acgcggagac
      841 cctcacccgt ttcctggcgc ccttcactcc cggcgacatc ccgttcgagc gcggcccctt
      901 cgaccggccg cagttcatcc tgtactcctc gggcaccacc ggcgtgccca agtgcatcgt
      961 gcacggcgcg ggcggcacgc tgctgcagca cctcaaggaa caccagctgc acacggacct
     1021 gaagcgcggc gagcggctgt tctacttcac cacctgcgga tggatgatgt ggaactggct
     1081 ggcgtcgggg ttggccacgg gtgccacgct gctgctgtac gacggctctc cgttccatcc
     1141 cgggccggcc gtgctgttcg atctcgccga cgccgagggg atgaccgtgt tcggcacctc
     1201 ggccaagtac atcgacgcgc tcggcaaggt cgggctgcgc ccgcgcgata ctcaccgcct
     1261 cgcgagcctg cgcaccatga cctcgaccgg ttcgccgctg tcgcccgaga gcttcgagta
     1321 tgtgtatcgc gcgatcaagc aggacctgct gctgtcgtcg atctccggcg gcaccgacat
     1381 cgtctcgtgc ttcgtgctcg gcagcccgct gctgccggtg tggaagggcg agatccagtg
     1441 ccgcgggctg ggcctgaagg tggaggtgta cgacgacgac ggccggccgg tgatcggcga
     1501 aaaaggcgag ctggtgtgca gcgccccgtt cccttcgatg ccggtcgact tctggaacga
     1561 ccccgacgga gccaagtacc gggaggccta cttcgcgcgc ttccccggcg tctggtgcca
     1621 cggcgactac atggagctga cgccgcgcgg gacggcggtg atttacgggc gctcggatgc
     1681 ggtgctcaac ccgggcggcg tgcgcatcgg caccgccgag atctaccgcc aggtggagca
     1741 gctgccggag gtggtggaga gcctggtgat cggccaggac tgggagcgcg acgtgcgcgt
     1801 ggtgctattc gtgcggctgc gcgacgggct cacgctggac caggcgctgg tggacaagat
     1861 caagcggcag atccgcacca acaccacgcc gcgccacgtg ccggcgaaga tctgccaggt
     1921 ggaggacatt ccacgcacca agagcggcaa gatcgtggag ctggccgtgc gcaacgtcgt
     1981 gcacgggcgg ccggtgaaga accgcgaagc gctggccaac ccggaggcgc tggcgttgtt
     2041 cgagaacctg ccggagctcc agcggtgaag aggctgaaga ggaagatgcg acgaaagcgg
     2101 gcggccggca gagtcgagag ccgccccaac cacacggcca cgccctgccg gcgcctcacc
     2161 gtcatcgtca tctcgtccaa aggcgcctac acgccggcat tcctcgggac ggaaatgtta
     2221 acggcaagtc tcgcggcggc ttaaggccga ttccggcgaa tcgttatgga aaccgccgcc
     2281 atgatacaaa tttacttgaa atacaatgtg atatggatca gacgcgtttg cggatagagg
     2341 gcatgacctg cccgggctgc gtgcgcagcg tcaaacgggt gctggaggcg gtggccgggg
     2401 tgcgtggcgc cgcggtctcc ctggaacagg gcgaagcggt ggtggacttc gatcccgatc
     2461 gcgccagtac cgccgatctg gtgcacgcgg tcgaaggcgc cggctaccgg gcgcagccgg
     2521 tgcaacgcgg atcttgacct accagaactg ccaccacttc ctctcctggc ggttcgccag
     2581 cttgtacttg gtgctcgggt agttgagctt gagcagccgg tcggcgtcgt cgcgcaactc
     2641 ggcaaggccc atggcgtcgt acgccttcac catcacgatc agcgcctgct cgaccgccgg
     2701 cgtcttgggg taattggtca gcacgtactg ggcgcgattc accgccgcga cataggcctt
     2761 gcgccgcagg taatactcgc cgacgcgcac ctcgttgttg gcgagcgcgt tgatcagata
     2821 gcgcatgcgc gcgtacgcgt cgggcacata gcggctgtcg ggaaagcgct gcaccagctc
     2881 gcggaagctc tcgaacgatt cgatcgccgc gcgcggatcg cgctcgctca tgtcctgatt
     2941 ggcgaaaccg ccgaacacgc ccaggtcctc gttgaagttg atcagcccct tcaggtagta
     3001 ggcgtagtcg acgttgggat gctcgggata gagcttgatg aagcggtcgg tctccgccag
     3061 cgccagcacc ggctcgacgt ccttgtagta ggcgtaagcc agctcgagct gcgactgctg
     3121 ggcatagcgg ccgtacggat agcgcgcctc gagcttctgg tagtactcga tggacttctt
     3181 gtagtcgccg ctcagcagct cggccttggc ctcgttgtaa aggcgctcgg cggaccagtt
     3241 gcgcgtctcg tcgtacttgt cgtcgccggt ggatctgcac gcgggcagca gcacgctgcc
     3301 gacgagcaac agcgccgatg cgaacccggc taaactacgc aacttgctca tgagcctcga
     3361 aaaacgtgcg cgcgattata gcgcatcgag cgccgcgccc gcacccgcgg cgctcggcaa
     3421 ggcgcgccac gcacgcgtgc ccgatgcgct cgccggtttg cgcctggacc aggcgctggc
     3481 gcggttgttc cccgagtatt cgcgcagcag gctgcagacc tgggtgaaga gcgcccgcgt
     3541 gacgctggac ggcgcgctgg cgcggccgcg cgacaaggtg cggggaggcg agcgggtcgc
     3601 gctgcgaccc gacgcggacc aaggcgcggc ccgcgatctc gcgcaggaca tcccgctcgg
     3661 catcctgttc gaggacggcg cg
//
LOCUS       DASPGE010000165         4614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_631348, whole genome shotgun sequence.
ACCESSION   DASPGE010000165 DASPGE010000000
VERSION     DASPGE010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631348"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /gene="mscL"
                     /locus_tag="VD791_05190"
     CDS             complement(<1..210)
                     /gene="mscL"
                     /locus_tag="VD791_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HXV07400.1"
                     /translation="MGMLKEFKSFAIKGNVVDLAVGVIIGVAFGKIVASMVEDVIMPV
                     LGTLIGGINFTGLAITVGSATIKYGK"
     gene            complement(230..781)
                     /gene="greB"
                     /locus_tag="VD791_05195"
     CDS             complement(230..781)
                     /gene="greB"
                     /locus_tag="VD791_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HXV07401.1"
                     /translation="MSKAFVRESESDDTAPLVAAEPSAPPRNYITPAGFARLDAERKQ
                     LVEVERPQVVRTVAWAAANGDRSENADYQYGKKRLREIDRRVRFLIKRLEGATVVNNA
                     GRDEERVFFGATVHVRTRDGGERQVSIVGADEVDAARGRVSWLSPIARALLKARAGDT
                     VTLRTPAGHEELEILDVSYPGID"
     gene            870..1601
                     /locus_tag="VD791_05200"
     CDS             870..1601
                     /locus_tag="VD791_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXV07402.1"
                     /translation="MALIQSSVEKDDLLTRLRIREVALFSLIALTAVLANLPEDYIQE
                     LGLNRDYLLAGLGSAVVIGLFLYLRFFFFFSVVLLFLGANLPEQIAQGFGISKIPLIL
                     ALVAMVGISLINYVVKLLPTGLEPKPKEKSPEGVRAMFYAIEKNNVPYGQRVMSMHFD
                     PNLHHDNGYTPLAYAALRGNLQMVEMFLRHGADPALPTREGETAVELALRAGHKEIAD
                     VLKRARQEREARGVPASAPAAGAAR"
     gene            1672..4506
                     /locus_tag="VD791_05205"
     CDS             1672..4506
                     /locus_tag="VD791_05205"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HXV07403.1"
                     /translation="MSTATLPAAPAIAPQEICRDALLEKYAKGGETSIAEVRARVARA
                     LAQAEPEARRTHWERRFLQALEDGFIPAGRINSAAGVQLQATLINCFVQPVGDSISET
                     VDGRPGIYTALQEAAETMRRGGGVGYDFSSIRPKGAEVKGTHSRASGPVSYMRVFDRS
                     CETVESAGARRDAQMGVLRCDHPDIEEFIHAKDRGELSNFNLSVGVTDAFMQAVEADA
                     QVELVHKAKPWPGPDVRRREDGLWVYRTLPARALWDQIMRSTYDHAEPGILFLDRMNR
                     DNNLNYCETIEATNPCAEQPLPPYGCCCLGSVDLTRFVRSPFAPEAAFDFDAFGKVVE
                     VAVRMLDNVLEVTAWPLEAQLREAMAKRRVGLGFTGLGDALIMLRLHYDSDAARAMAA
                     RIAEAMRDRAYLASVELAKERGAFPLYNADLYLSGTSFAARLPEEVKARVRAHGLRNS
                     HLLSIAPTGTISLAFADNASNGIEPPFSWTYTRKKRMPDGNLQEFPVEDHAWRRYKAA
                     GGDVEQLPPYFVTALELSAAAHEKMVAAVAPFIDTSISKTVNVPEDYPYAEFKDLYLA
                     AWKSGLKGLATYRPNKVLGSVLSVDKPQDFVEDDVNRRIRIKSVPEPVLASLRWPGRP
                     EFAAGNLSWTYMIEHPHHHFAVFVGEAENGGAHAFEVWVNGSEQPRGLGAVAKTLSMD
                     MRAHDPGWLRLKLDTLAKTSGDDAFDMAFPPHGEMRHMPSVVSALAQLVRWRVEQLGG
                     LAGESQADAFASVTPLLDAMFSLKEPKTGTDGTMSWTVDVHNPRTGDDFVLGLKEIAL
                     PDGTTRPYSMWLSGEYPRALDGLCKILSLDMRVLDPAWIGMKLRKLLDFPEPLGDFMA
                     FVPGSRKQQTFPSTVSYLARLILHRYEMLGILDEQGHPKTQMGILEVPSKTHSPLIAG
                     SRCPECGNTTLIRKDGCDYCTACGYVGVCG"
     gene            complement(4503..4604)
                     /locus_tag="VD791_05210"
     CDS             complement(4503..4604)
                     /locus_tag="VD791_05210"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HXV07404.1"
                     /translation="MEWARANGRKLMPLCPFAKSVFDKTPEYRDVLA"
BASE COUNT          712 a         1662 c         1531 g          709 t
ORIGIN      
        1 cttgccgtac ttgatggtcg ccgatcccac ggtaatcgcc aggccggtga agttgatgcc
       61 gccgatcagc gtgccgagca ccggcatgat tacgtcctcg accatcgagg ccacgatctt
      121 gccgaaggcc acgccgatga tcacgcccac cgccaggtcg acgacgttgc ccttgatggc
      181 gaaactcttg aattccttca gcatgcccat gcgcggctcc tctggttgtt caatcgatgc
      241 ccggatagct cacgtccaga atctccagct cttcgtgccc ggccggcgtg cgcagcgtta
      301 ccgtgtcgcc ggcgcgcgcc ttgagcagcg cgcgggcgat gggcgagagc cacgacacgc
      361 gcccgcgcgc cgcatccacc tcgtcggcgc cgacgatgct cacctgccgc tcgccgccgt
      421 cccgcgtgcg cacgtgcacg gtcgcgccga agaatacccg ttcctcgtcg cgcccggcgt
      481 tgttcactac cgtcgcgccc tcgagccgct tgatcaggaa gcgcacgcgc cggtcgatct
      541 cgcgcaggcg cttcttgccg tactggtagt cggcgttttc cgagcggtcg ccgttggccg
      601 ccgcccaggc cacggtgcgc accacctgcg gacgctccac ctcgaccagc tgcttgcgct
      661 cggcgtcgag gcgcgcgaag cccgccggcg tgatgtagtt gcgcggcggc gcgctcggct
      721 ccgccgccac cagcggggcg gtgtcgtcgc tctcggattc gcggacaaac gctttcgaca
      781 tgcggcccca ttctaccggc cgccgacttg ccacagcgcg ggggaccgcc gaaaatccgc
      841 cctgcgatga ggcgacgccc ggaggccgtg tggcattgat tcagagttcc gtcgagaagg
      901 acgacctgct gacccggctg cgcatccggg aagtggcgct gttcagcctg atcgcgctca
      961 ccgcggtgct cgccaacctg cccgaggact acatccagga gctcggcctg aaccgcgatt
     1021 acctgctggc cgggctgggc agcgcggtgg tcatcggcct gttcctctac ctgcgcttct
     1081 tcttcttctt ttccgtggtc ctgctgttcc tcggcgccaa cctgcccgag cagatcgcgc
     1141 agggcttcgg catctccaag atcccgctga tcctcgcgct ggtcgccatg gtcggcatct
     1201 ccctcatcaa ctacgtggtc aagctgctgc ccacgggcct ggagcccaag cccaaggaga
     1261 agagcccgga gggcgtgcgc gcgatgttct acgccatcga gaagaacaac gtgccctacg
     1321 ggcagcgggt catgtcgatg cacttcgacc ccaacctgca ccacgacaac ggctacacgc
     1381 cgctcgccta cgccgcgctg cgcggcaacc tgcagatggt ggagatgttc ctgcgccacg
     1441 gggcggatcc cgcgctgccc acgcgcgagg gcgaaaccgc ggtcgagctg gcgttgcgcg
     1501 ccgggcacaa ggagatcgcc gacgtgctca agcgcgcccg ccaggagcgc gaggcgcggg
     1561 gcgtgcccgc ctccgccccc gccgcgggcg cggcgcgctg agcgcccccg ggccgacttg
     1621 cgggcgccgg ccgatggcgc gacaatgctg tacggatatg cagtaaagca catgagcacc
     1681 gccacccttc ccgccgcccc ggcgatcgcc ccgcaggaga tctgccgcga cgccctgctg
     1741 gagaagtacg ccaagggggg cgaaacctcg atcgccgagg tgcgcgcgcg cgtcgcgcgg
     1801 gcgctcgccc aggccgaacc cgaggcgcgc cgcacgcact gggagcggcg cttcctgcag
     1861 gcgctcgagg acggcttcat ccccgcgggg cgcatcaatt cggccgccgg ggtgcagctg
     1921 caggccacgc tcatcaactg cttcgtgcag ccggtcggcg actcgatctc cgagaccgtc
     1981 gacggcaggc ccggcatcta cacggcgctg caggaagccg ccgaaacgat gcggcgcggc
     2041 ggcggcgtgg gctacgactt ctcctccatc cgccccaagg gcgccgaggt gaagggcacc
     2101 cactcgcgcg ccagcggccc ggtgtcctac atgcgcgtct tcgaccgttc ctgcgaaacg
     2161 gtggaatcgg ccggcgcgcg ccgcgacgcg cagatgggcg tgctgcgctg cgaccacccg
     2221 gacatcgagg aattcatcca cgccaaggac cggggcgagc tgtcgaattt caacctctcg
     2281 gtcggcgtca ccgacgcctt catgcaggcc gtggaagccg acgcacaggt cgagctggtg
     2341 cacaaggcga agccctggcc ggggccggac gtgcgccggc gcgaggacgg gctgtgggtg
     2401 taccgcacgc tgccggcgcg cgcgctctgg gaccagatca tgcgctcgac ctacgaccac
     2461 gccgagccgg ggatcctgtt cctcgaccgc atgaaccgcg acaacaacct gaactactgc
     2521 gagacgatcg aggccacgaa cccctgcgcc gagcagccgc tgccgcccta cggttgctgc
     2581 tgcctgggct cggtcgacct gacgcgcttc gtgcgctcgc cgttcgcccc ggaggcggcg
     2641 ttcgactttg acgccttcgg caaggtggtc gaagtcgccg tgcgcatgct ggacaacgtg
     2701 ctcgaggtca cggcctggcc gctcgaggcg cagctgcgcg aggcgatggc caagcgccgc
     2761 gtgggcctgg gattcaccgg cctcggggac gcgctcatca tgctgcggct gcactacgac
     2821 agcgacgcgg cgcgcgccat ggcggcgcgc atcgccgagg cgatgcgcga ccgcgcctac
     2881 ctggcgtccg tcgagctggc gaaggagcgc ggcgcgttcc cgctgtacaa cgccgacctg
     2941 tacctctcgg gcaccagctt cgccgcgcgc ctgccggagg aggtgaaggc gcgggtgcgc
     3001 gcgcacggcc tccgcaacag ccatctcttg tcgatcgcgc ccacgggcac catcagcctg
     3061 gcgttcgccg acaacgcttc gaacggcatc gagccgccgt tttcctggac gtatacgcgc
     3121 aagaagcgca tgcccgacgg caatctgcag gagttcccgg tcgaagacca cgcctggcgc
     3181 cgctacaagg cggccggcgg ggacgtggag cagctgccgc cgtacttcgt gaccgccctg
     3241 gagctgtccg ccgccgcgca cgagaagatg gtcgccgcgg tggcgccgtt catcgacacc
     3301 agcatctcca agaccgtgaa cgtgccggag gactatccct acgcggagtt caaggacctg
     3361 tacctcgcgg cctggaagtc cggcctgaag gggctcgcca cctaccggcc gaacaaggtg
     3421 ctcggctcgg tgctgtcggt cgacaagccg caggacttcg tcgaggacga cgtcaaccgc
     3481 cgcatccgca tcaagtcggt gcccgagccg gtgctcgcca gcctgcgctg gccgggccgg
     3541 cccgagttcg cggcgggcaa cctctcctgg acgtacatga tcgagcaccc gcaccaccac
     3601 ttcgccgtgt tcgtcggcga ggcggagaac ggcggggcgc atgcgttcga ggtgtgggtc
     3661 aacggcagcg agcagccgcg cgggctgggc gccgtggcga agaccctgtc catggacatg
     3721 cgcgcgcacg atcccggctg gctgcgcctc aagctcgaca cgctggccaa gacctccggc
     3781 gacgacgcct tcgacatggc cttcccgccg cacggcgaga tgcgccacat gccgtcggtg
     3841 gtgtccgcgc tggcgcagct cgtgcgctgg cgcgtagagc agctcggcgg cctggccggc
     3901 gagtcgcagg cggacgcgtt cgccagcgtc acgccgctgc tcgacgcgat gttcagcctc
     3961 aaggagccga agaccggcac cgacggcacg atgtcctgga ccgtggacgt gcacaacccg
     4021 cgcaccggcg acgacttcgt gctcgggctg aaggagatcg cgctgcccga cggcaccacc
     4081 cgtccttact cgatgtggct ctcgggcgag tacccgcgcg cgctcgacgg cctgtgcaag
     4141 atcctgtcgc tggacatgcg cgtgctcgac ccggcctgga tcggcatgaa gctgcgcaag
     4201 ctgctcgact tcccggagcc gctgggcgac ttcatggcct tcgtgcccgg cagccgcaag
     4261 cagcagacct tcccctcgac ggtctcgtac ctcgcgcggc tgatcctgca ccgctacgag
     4321 atgctcggaa tactggacga gcaaggccac ccgaagacgc agatgggtat tcttgaagtt
     4381 ccttcaaaaa cccactctcc gctgatcgcc ggcagccgtt gccccgagtg cggcaacacg
     4441 acgctcatcc gcaaggacgg ctgcgactac tgcaccgcgt gcggctacgt cggggtgtgc
     4501 ggctaggcga gcacgtcccg gtattccggg gtcttgtcga agaccgattt cgcgaacggg
     4561 catagcggca tcagcttgcg gccgttcgcg cgcgcccatt ccacggccgc tttc
//
LOCUS       DASPGE010000166         7318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1450549, whole genome shotgun sequence.
ACCESSION   DASPGE010000166 DASPGE010000000
VERSION     DASPGE010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450549"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VD791_05215"
     CDS             <1..317
                     /locus_tag="VD791_05215"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HXV07405.1"
                     /translation="LFSPGLYVHRKAIAEAALAQRFPTVFEISSMVEAGGLISYGPDP
                     SYGWRRAGYFVSRILAGVPPSELPIEQPREYRLVVNARTAKAVGITVPESILLRADEV
                     IR"
     gene            complement(686..1666)
                     /locus_tag="VD791_05220"
     CDS             complement(686..1666)
                     /locus_tag="VD791_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HXV07406.1"
                     /translation="MTRIAVLDDWQGIARTSADWSDLQRRADVVFFEQPLGGLDAAAR
                     ALAEFDILLAMRERTAFPAALVQRLTRLRMLSVTGSRTRSLDLDACTARRILVCGTGG
                     ERSGAATAELALGLLLAATRHVARADASTRAGGFQSGVPVGSVLEGRTLGIVGLGRIG
                     ARMARYAVALGMQLLAWSQNLTQQRARAAGARLVDKAELLAESDAVSLHLVLSDRTLG
                     ILGAADLARMKPGAILVNTSRGPLVDEQAMLERLRAGKLTAALDVYGEEPLPADHPLR
                     SLPNVVLTPHLGYCTHEVYAQFYRESIENALAYLEGKPMRAFNPEVAQGR"
     gene            1737..2078
                     /locus_tag="VD791_05225"
     CDS             1737..2078
                     /locus_tag="VD791_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07407.1"
                     /translation="MIKVVRLHTGSDNQSHFEDIEIPLADAGKTGFLSERVKATGVIF
                     RITTGECNYEFRPAPRRQYVVNLEGEGSRRTNWAASAPARLPCVVDIPYDFSGIAAGG
                     SRRYDSPANRA"
     gene            2129..3034
                     /locus_tag="VD791_05230"
     CDS             2129..3034
                     /locus_tag="VD791_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HXV07408.1"
                     /translation="MLDKIWIAMLCLGACGPALAEEPASADDAQQREAAARQFEASLR
                     FQEGEITLPGGIAALQVPTGFRYVGPDDARRVLEDGWGNPKGDGTLGMLFPADVSVVG
                     ADGWGVVITYEEDGHVKDADADSIDYDELLADMREGVAAENDARRDAGYEAIELVGWA
                     AKPHYDKAARKLYWAKELKFGDSADHTLNYNIRILGREGVLVLNAVSDMGQLGKVERD
                     MQQVLAFTNFTAGNRYEDFDASIDKVAAYGLGALVAGGVAAKTGLLAKLLALLIAFKK
                     FIILGAVVAAGVISKVLFGRKDQSA"
     gene            complement(3047..5737)
                     /locus_tag="VD791_05235"
     CDS             complement(3047..5737)
                     /locus_tag="VD791_05235"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXV07409.1"
                     /translation="MPTVTFAAVWPLALLLVVPLVWLLAGRARSLSGGARITGAAVLR
                     SAALAAIVVALMRPTLHWTSEDVSVVYALDVSSSVSKRFLDDALDWIARIDGRHRPAQ
                     SHFVAFGDRAELLGSVAEVRALTLATGDAAAPGDDSIHQGATNLEEALLRALPAFAPG
                     HARRIVLVSDGNQTEGDVWRAMLRLKAERVRVFAVPAAVSADSDAWVEAIAVPEGVRE
                     GAAVDVEARLFSRLPVPARVELSIGGRAATARAVTLSPGENRVTLNVRFARAGAQDIT
                     VKVSAEGDQLARNDALTEDVFVQPRPHVLYVEGGPEGTHHLADALTAQGIRVSVATVQ
                     ALSADPRLLAGKDAVVLSDVRAESLGAGAAQRLQAFVRDGGGGLIFAAGENTYGQDGF
                     AKSEVERLLPVKFEAKRKRQDLDLVLLIDRSASMRGGKIEVAKAAALATLDLLEEEQG
                     LGVVAFDAQPHEIVPLGPVGDKREAENLIARMTSSGQTDIYRALLHAQRSFAESRAEI
                     KHIILLSDGLTAPPPADAVGASGQGSRGNRINLQESQWPRYDDDMAGSFRGLVAQLAA
                     AKVTLSTVVMGEGPDVKFMTALAQMGHGRTYIARSDGEVPSLFAGETRRVRGDAVVEE
                     PFRPLVKAWSPTIAGVDFAGGPQLRGYVESKPKRFSDVLLEAKKDLPLLAETHYGLGK
                     TVVFLSDVKNRWATDWLGWPGYAKLWGQVVRDSVRRDAGDGVRWGVTREGRSALVQLT
                     ALGDDGGHRSGLQPRVRITPPSGDSSVAELPQIAPGRYATRIPLAGASSAPWHFELLP
                     GADLRTADLARAGARRLFYTYPDEYRLLPPNLPLLRALSEQTGGALAPRADEIFRPRG
                     DGGITTTPLWPGFVATALLLFLLDILVRRAPWRWSLRRGR"
     gene            complement(5742..>7318)
                     /locus_tag="VD791_05240"
     CDS             complement(5742..>7318)
                     /locus_tag="VD791_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07410.1"
                     /translation="MALALTRPEVPVIAASAQRLLLILDNSPSMAARMRDGGSRWQHA
                     LEEAGALLRQSGAPSQVMLLDTVGQLRGFGFVDRDSALASLGRASVASWGIARMPPAP
                     LPAGAQVHLFTDGVAPIEVPEGAIVHRIFEPADNVAVTAFSARPLAQDPTRYEAVVQV
                     LNASPGEQRVRLLITGGAQFSIAQDIDLQAGETINAVFDVSDFEGGVLGATVICEADD
                     FALDDSAYAMIPPHRPKRVLLVTAGNPALGDALRSLPGVRLTVVPPAGYVRAGAHDAF
                     VFDRFAPLEPPPAGALLLRPPARKWLAGKPTPRNGPRIATWNEDHAVSGAIAWRNLRL
                     ARASLEAATRTDAHALVLAHGSASGAVVTAGEAPVRWVKVGFALEDSNFALQPDFPVF
                     LGNALSWVTEPAPVLARGLGSVQVALPHARVHDGSGKPVAASPTSEGVVFEAPRADVY
                     TVSGSGGRVLVVANVAEQSYALINRTRLADGHTAPIADEAPAPLWPAELWMLLLLFAG
                     ALLLAEWAVFTRRRRN"
BASE COUNT         1140 a         2570 c         2529 g         1079 t
ORIGIN      
        1 tactattttc gcctggtctc tacgtccacc gcaaggctat cgccgaggcg gccctagcgc
       61 agcgattccc caccgtcttc gagatctcct cgatggtcga ggccggcggg ctcatctcgt
      121 atggtccaga tccatcctac gggtggcggc gagccggata ctttgtaagc cgcatcctcg
      181 cgggggtccc tcccagcgaa ttacccatag aacagccgag agagtaccga ttggtggtaa
      241 atgccaggac cgcgaaagca gtcggcatta cagtgccaga atccattctt ctgcgggccg
      301 acgaggtcat ccgatgaact tgacgccgaa catcgcgttg taccggagcc gcaacagcgg
      361 tgctgcgctt gctgttgcgg cccggtgaac gcggtcgttc ggcgtcctgt ggtggtgctg
      421 gtttgtcaca gtacgtccat cgtcagtcac ggcttacgtg ctggcgatgc atcgcgtgcg
      481 gccccctatc gggcaacttg acgagcttgg ccccgatccg ccgcacccgg tcccaccgcg
      541 gcgccgttgg gcccgccgcc gagtgcagga agacgtggat cctgccgtgg agcgtgacgc
      601 gcgacagggt gcgtcgcttg tactcgacca ggatcaccga gccgcccggg atcgcgaccg
      661 ggctttccgg aagccgcagc cccgctcacc ggccctgcgc aacctccggg ttgaacgcgc
      721 gcatcggctt gccttccagg tacgccagcg cattctcgat ggattcccgg tagaactgcg
      781 cgtacacctc gtgcgtgcaa tagcccagat gcggcgtcag taccacattg ggcaggctgc
      841 gcagagggtg atcagcgggc aacggctcct ctccatacac gtccagtgcg gcagtcagct
      901 tgccggcacg cagccgttcc aacattgcct gctcatccac cagcggtccg cgcgaggtat
      961 tcaccaggat ggcgcccggc ttcatgcgtg ccaggtcggc cgcgccgagg atgccgagcg
     1021 tgcggtcgga gagcacaaga tgcaggctca cggcgtcgga ctcggccagc agttcggcct
     1081 tgtccaccag gcgcgcgccg gccgcgcgcg ccctctgctg ggtgaggttc tggctccatg
     1141 ccagcagctg catgccgagc gccacggcgt agcgtgccat tcgcgccccg atccggccca
     1201 acccgacgat cccgagcgtt cggccttcga gcacgctgcc gacgggtacg ccggactgga
     1261 atccaccggc acgggtggat gcatcggcac gcgcaacatg ccgggtcgcg gccaggagca
     1321 gacccagcgc cagctctgcc gtggcggcgc ccgaacgctc gcctcctgtg ccgcacacca
     1381 gaatcctgcg tgccgtgcac gcgtcgagat ccaggctgcg ggtgcgggag ccggtgacgc
     1441 tgagcatccg caggcgcgtc aggcgctgca ccagcgctgc ggggaacgcg gtgcgctcgc
     1501 gcatcgccag caggatgtcg aactcggcca gcgcgcgtgc ggccgcatcc agcccgccca
     1561 gcggctgctc gaagaacacc acgtctgcgc gccgctgcag gtcggaccag tcggcgctgg
     1621 tgcgcgcgat gccttgccag tcgtccagta cggcgattcg cgtcatttgc caatgagaag
     1681 gtcagcgtgc tagcgccact ctacaataca cgagcgcagc cgaggaggga ggaccgatga
     1741 tcaaggtggt gcgtctgcat accgggtcgg acaaccagtc gcatttcgag gacatcgaga
     1801 tcccgctcgc tgacgccgga aaaaccggct tcctgtccga gcgggtcaag gccaccggtg
     1861 tcatcttccg catcaccacc ggtgaatgca actacgagtt tcgccctgcg ccgcggcgac
     1921 aatacgtggt gaacctggag ggcgaggggt cgcgtcggac caactgggcc gcatctgccc
     1981 ctgcgcgttt gccgtgcgtg gtcgatatcc cctatgactt ttcggggata gccgcgggcg
     2041 ggtcgcgccg ctacgattcg cccgccaatc gggcctgatg cgcgacgccg tcgcgcccgt
     2101 gccgccctct ctggccaagg agagaacgat gctcgacaag atctggattg cgatgctgtg
     2161 cctgggcgcc tgcggaccgg cgctggccga ggaaccggct tccgccgacg atgcgcagca
     2221 gcgcgaggcg gctgccaggc agttcgaggc ttcgctgcgc ttccaggagg gggagatcac
     2281 cttgccgggc ggcatcgcgg cgctgcaggt gccgaccggg ttccgctacg tcggccccga
     2341 cgacgcgcgc cgcgtgctcg aggacggctg gggcaacccc aagggcgacg gcacgctcgg
     2401 catgctgttt ccggccgacg tctcggtggt cggcgcggac ggctggggcg tggtgatcac
     2461 ctacgaggag gacgggcacg tcaaggacgc ggatgccgac agcatcgact acgatgagct
     2521 gctggccgac atgcgggagg gtgtggcggc cgagaacgat gcgcgccggg acgcgggcta
     2581 cgaggcgatc gagctggtcg gctgggccgc caagccgcat tacgacaaag cggcgcgcaa
     2641 gctgtattgg gccaaggagc tgaagttcgg cgacagcgca gaccacacgc tcaactacaa
     2701 catccgcatc ctggggcgcg agggcgtgct ggtgttgaac gccgtgtccg acatgggcca
     2761 gctcggcaaa gtggagcggg acatgcagca ggtgctcgcc ttcaccaact tcaccgccgg
     2821 caaccgctac gaggatttcg acgccagcat cgacaaggtg gccgcttacg gcctgggtgc
     2881 tctggttgcg ggcggcgtgg ccgccaagac cggcctgctg gcgaagctgc tggcgctgct
     2941 gatcgcgttc aagaaattca tcatcctggg cgcggtcgtg gccgctggtg tgatcagcaa
     3001 ggtgctgttc ggccgcaagg atcagtccgc ctagccgggg ctgcggctaa cgcccgcggc
     3061 gcaggctcca cctccagggc gcgcgccgca ccaggatgtc cagcaggaac agcagcaggg
     3121 ccgtggcgac gaagccgggc caaagcggcg tggtcgtgat gccgccgtcg ccgcgcggcc
     3181 ggaagatctc gtccgccctg ggcgccagcg ccccgccggt ctgctcgctc agggcgcgca
     3241 gcagcggcag gttcggcgga agcaggcggt actcgtccgg gtaggtgtag aagagcctgc
     3301 gggcgccggc gcgcgcaaga tcggcggtgc gcaagtccgc gccgggcagc agctcgaaat
     3361 gccagggcgc gctgctcgca ccggcgagcg ggatgcgcgt cgcgtagcgc ccgggcgcga
     3421 tctggggcag ctccgcgacg gagctgtctc cgctgggcgg cgtgatgcgc acccgcggct
     3481 gcaggccact gcggtggccg ccgtcatcgc cgagcgcggt cagctggacc agcgcgctgc
     3541 gcccctcgcg cgtcacgccc cagcgcactc catctccggc gtcgcgccgc acgctgtcgc
     3601 gcaccacctg accccagagc ttggcgtaac ccggccagcc gagccaatcg gtcgcccagc
     3661 ggtttttcac gtcggacagg aacaccacgg tcttgcctag accgtaatgc gtttcggcca
     3721 gcagcggcag gtccttcttg gcctcgagca gcacgtcgga gaagcgcttg ggtttgctct
     3781 ccacgtaacc gcgcaactgc ggtccgcctg cgaaatccac gccagcgatg gtcgggctcc
     3841 aggccttcac cagcggccgg aacggctcct cgacgacggc gtcaccgcgc acgcgccgcg
     3901 tctctcccgc gaacaggctg ggcacctcgc cgtcgctcct ggcgatgtag gtcctgccgt
     3961 gccccatttg cgccaaggcg gtcatgaact tcacgtccgg gccttctccc atgaccacgg
     4021 tggacagcgt gaccttcgcg gcggcgagct gcgcgacaag gccccggaag ctgccggcca
     4081 tgtcgtcgtc gtatctcggc cattggctct cctgcaggtt gatccggttg ccacgcgaac
     4141 cctggccact ggcgcccacg gcatcggcgg gcggcggggc ggtgagcccg tcggacagca
     4201 ggatgatgtg cttgatctcg gcccgcgact cggcgaacga gcgctgcgcg tgcagcagcg
     4261 cacggtagat atcggtctgg ccgctggagg tcatgcgcgc gatcaggttc tcggcctcgc
     4321 gcttgtcgcc caccgggccg agcggcacga tctcgtgcgg ttgcgcatcg aacgccacga
     4381 cgcccaggcc ttgctcctct tcgagcagat ccagcgtcgc gagcgcggcg gccttcgcca
     4441 cctcgatctt gcccccgcgc atgctggccg agcggtcgat cagcagcacc aggtccagat
     4501 cctggcgctt gcgcttggcc tcgaacttga ccggcagcag ccgttccacc tcgctcttgg
     4561 cgaagccgtc ctgcccatag gtgttctcgc ccgcggcgaa gatcaggccg ccgccgccgt
     4621 cgcgcacgaa cgcctgcagg cgctgcgcgg cgccggctcc gagactttcc gcgcgcacgt
     4681 cgctcaggac gacggcgtcc ttgcccgcga gcaggcgcgg atcggcggac agggcctgca
     4741 ccgtggcgac cgacacgcgg atgccttgcg cggtgagcgc gtccgccaga tgatgcgtgc
     4801 cctccgggcc gccctccacg tacagcacgt gcggccgcgg ttgcacgaag acgtcctcgg
     4861 tgagcgcgtc gttgcgcgcg agctggtcgc cttccgcgct cacctttaca gtgatatcct
     4921 gtgcgccggc gcgcgcgaag cgcacgttca gcgtcacgcg gttctcgccc ggcgagagcg
     4981 tgaccgcgcg tgcggtggcc gcgcgcccgc cgatgctcag ctcgacccgt gccgggacgg
     5041 gcaggcgcga gaacaaccgc gcttcgacat ccaccgcggc gccttctcgc acgccctccg
     5101 gcacggcgat cgcctcgacc catgcgtcgc tgtcggccga taccgccgcg ggaacggcga
     5161 acactcgcac gcgttcggcc ttcaggcgca gcatcgcgcg ccacacgtcg ccctcggtct
     5221 gattgccgtc gctcaccagc acgatgcgcc tggcgtggcc gggcgcgaac gcgggcagcg
     5281 cccgcaacag ggcttcttcc aggttggtgg cgccttgatg gatcgaatcg tcgcccgggg
     5341 cggccgcgtc accggtagcc agcgtgagcg cgcgcacctc ggcgaccgac ccgagcagct
     5401 cggcgcgatc gccgaacgcg acgaagtgcg actgcgcggg ccgatggcgg ccgtcgatcc
     5461 gtgcgatcca gtcgagcgcg tcgtcgagga atcgcttcga aacgctgctg gacacgtcca
     5521 gcgcatacac caccgacaca tcctcgctgg tccagtgcaa cgtgggccgc atcagcgcga
     5581 cgacgatcgc ggccagcgca gccgagcgca ggacggcggc gccggttatg cgcgcgcccc
     5641 cggagagaga gcgcgcgcgc ccggcgagca gccagacgag cggaaccacc agcagcagcg
     5701 caagcggcca aacggcggcg aacgtgacgg tcggcatgcg atcagttcct gcgccgccgt
     5761 gtgaacacgg cccactccgc gaggagcaat gcgcctgcga acagcagcag cagcatccac
     5821 agctccgccg gccagagcgg agcgggagcc tcgtctgcga tcggggccgt atgaccgtcg
     5881 gcgagccggg tccggttgat cagcgcgtag ctctgctcgg ccacgttggc cacgaccagt
     5941 acccgcccgc cggagccgct gacggtgtaa acgtccgccc gcggcgcctc gaacaccacg
     6001 ccctcggagg tgggcgaggc cgcgaccggc ttgccgctgc cgtcgtgcac ccgggcgtgc
     6061 ggcagtgcga cttgcacgct gccgagcccg cgcgcgagca ccggcgcggg ttcggtgacc
     6121 cagctcaagg cgttgccgag gaacaccggg aagtccggct gcaacgcgaa attggaatcc
     6181 tccagcgcga agccgacctt gacccagcgc accggcgcct cgcctgcggt caccaccgca
     6241 ccggaggccg acccatgggc cagcaccagc gcatgggcat cggtcctggt ggcggcctcg
     6301 agcgatgcgc gcgcgagccg aaggttgcgc cacgcaatcg ctccgctgac cgcgtgatcc
     6361 tcgttccagg ttgcaatgcg cggcccgttg cgcggcgtcg gctttcccgc cagccacttg
     6421 cgcgcaggcg ggcgcaacag caaggcgccg gccggcggcg gctcgagcgg agcgaagcga
     6481 tcgaacacga aggcgtcgtg cgcgccggcg cgcacgtagc cagccggcgg gacaaccgtg
     6541 aggcgcaccc cgggcaagct gcgtaacgcg tccccgagcg ccggattgcc ggcggtcacc
     6601 agcagcacgc gcttcggtcg gtgcggcggg atcatcgcgt aagccgagtc gtccagtgcg
     6661 aaatcgtcgg cttcgcagat cacggttgcg ccgagcacgc cgccttcgaa atcggagacg
     6721 tcgaacaccg cgttgatcgt ctcgccggcc tgcaggtcga tgtcctgcgc gatcgagaac
     6781 tgcgcgccgc cggtgatcag cagccgcacg cgctgctcgc cgggcgaggc gttcagcacc
     6841 tgcacgacgg cttcatagcg ggtcggatcc tgcgccaggg gacgcgcgga gaaggcggtc
     6901 accgcgacgt tgtccgccgg ttcgaagatg cggtgcacga tcgcgccctc cggcacctcg
     6961 atcggcgcga cgccgtcggt gaacaggtgc acctgcgcgc cggcgggaag cggcgcgggc
     7021 ggcatgcgcg cgatgcccca ggaagctacc gacgcccggc cgagcgaagc gagcgccgaa
     7081 tcgcgatcga cgaaaccgaa gccgcgcagc tggccgaccg tgtccagcag catcacctgg
     7141 ctcggcgccc ccgattgccg gagcagcgcg ccggcctcct cgagcgcgtg ctgccagcgg
     7201 ctgcccccgt cgcgcatccg cgctgccatg gaaggggaat tgtccaggat cagcagcagc
     7261 cgctgtgcgc tggcggcgat cacggggacc tcgggccggg tgagcgcgag cgccatgc
//
LOCUS       DASPGE010000167         9441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_514783, whole genome shotgun sequence.
ACCESSION   DASPGE010000167 DASPGE010000000
VERSION     DASPGE010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9441
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514783"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VD791_05245"
     CDS             complement(<1..535)
                     /locus_tag="VD791_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueG-associated DUF1730 domain-containing
                     protein"
                     /protein_id="HXV07411.1"
                     /translation="MQATSVDSPSADIDLVALKRSVAGWARDLGFERMGVSGIDVDAA
                     QARLLEWLGRCYHGEMGYLERHAALRGRPDVLLPGTLRVLSLRMNYFPAGARDAQAVL
                     ADASLAYISRYALGRDYHKVLRRRLLRLAARIEAQVGAFRYRVFTDSAPVMEVELAAQ
                     AGLGWCGKHTLLLDRSAG"
     gene            727..1677
                     /gene="ltnD"
                     /locus_tag="VD791_05250"
     CDS             727..1677
                     /gene="ltnD"
                     /locus_tag="VD791_05250"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HXV07412.1"
                     /translation="MAKARKKIAAGVIGLGSMGWGVARTLLNKGFTVYAYDVRPQVLK
                     DFARAGGKACTNPGEVGANAPVVITLVVNAEQTAEVLLGSAGAAPRMARGSVVIASAT
                     VAPAFAEELGARLNALGVHMIDAPVSGGAARAAAGQLSIMASGAREAFLRARPVLEAV
                     AAKVYRLGDAPGMGSKVKMVNQLLAGVHIAASAEAMALGIRAGVDPNVLYEVISNSAG
                     SSWMFQNRVPHILAGDYTPLSAVNIFVKDLGIVLDSARKATFPLPLTATAHQMFLMAG
                     AHGHGQEDDSAVVKVFQAVSGIPLPEKKRAPRPRTAARRK"
     gene            complement(1681..2367)
                     /locus_tag="VD791_05255"
     CDS             complement(1681..2367)
                     /locus_tag="VD791_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV07413.1"
                     /translation="MLLRRALSWVLSRAVSRAVSRAWLLCLLLAAGAAAGELTREQAL
                     MALDSPDATARREAVTELGRTGGMADAGALIGHLRDEDADVRALAERAVWQVWARSGD
                     PEVDGLLQRGIRHMEDGQMGQAVDAFTRVIDLRPDFAEGWNKRATAYYLIGDYDQSLK
                     DCDQVIERNPSHFGALSGYGLIYVQLGELERALEYFERALDINPNLEGVEQSIALIRY
                     RLGQSGKQSI"
     gene            2524..3675
                     /locus_tag="VD791_05260"
     CDS             2524..3675
                     /locus_tag="VD791_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HXV07414.1"
                     /translation="MNAPREPAPAQALPPPAQAGMRLEQVDTPALLLDLDAFERNLRR
                     LHASLGDAKVRVRPHAKSHKCPQIALRQIALGAVGVCCQKVSEAEALVRDGVGDVLIA
                     NEIVGAQKLARLTALARSAKIGVCVDDIANVADLDRAAREAGARLNVYVEMNVGANRC
                     GVEPGEPALRLAQAVARAGALRFAGVQAYQGAAQHLRSAAERSAAIAKAVEQVRETVA
                     LLEANGLRAEIVTGAGTGTYLLEAASRVYNEIQPGSYVFMDADYGRNLGEDGQPVHEF
                     AQSLFILATVMSRPTPQRAVVDAGLKAHSVDSGMPAVVGIAGARYVRASDEHGVIELG
                     GSGTAGLGQKIRLIPGHCDPTVNLYDWLVCYRGERVEDIWPISARGAFY"
     gene            3689..4984
                     /locus_tag="VD791_05265"
     CDS             3689..4984
                     /locus_tag="VD791_05265"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HXV07415.1"
                     /translation="MPAPRYLSFDTLSLHAGQRPDPATGARATPIYQTSSYVFRDTDH
                     AAALFNMERAGHVYSRISNPTNAVLEERIAALEGGIGGIATASGQAALHLAVATIAGA
                     GSHIVSSRSLYGGSHNLLHYTLPRFGIETTFVDPRDLDQWRTAMRPNTRLLFGETLGN
                     PGLDVLDVPAVAQIAHDAKIPLLVDATFTTPYLMRPFELGADLLYHSATKFLGGHGIV
                     IGGLLVDSGAFDWEASGKFPELTEPYSGFHGMDFQEESTVAAFLLRARREGLRDFGAC
                     MAPTTAFHILQGIETLGLRMARHVDNTRKVVQFLVGHPAVESVGYPELPTHPDHEIAK
                     RLLPRGCGAVFPFSIRGGRPAGRKFIESLRVFSHLANVGDAKSLVIHPATTTHFRMDD
                     AALAAAGIGPGTIRLSIGLEDPQDLIDDLAGALRAAQKV"
     gene            5031..5819
                     /locus_tag="VD791_05270"
     CDS             5031..5819
                     /locus_tag="VD791_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXV07416.1"
                     /translation="MDLRVDGKQAYAYTGGRPFDGARPAVVFIHGAEHDHCVWVLQSR
                     YLAHHGRSVLAVDLPGHGRTAGPPLASVEALADWIVALLNAAGAPKAALVGHSMGSLI
                     AVECAARHPERISKIALVGTAFPMRVAPELLEATRSAEPLAQDMVNIWSHSSYAQYPS
                     NPGPGFWVMGENLRLMQRQKPGVMHVDFAACNAYANGVQAAAQVVCPALLVLGKRDIM
                     TPPRATAELTAALRDARVVEIPGTGHALMAEKPDEVLDALAAFL"
     gene            5834..6550
                     /locus_tag="VD791_05275"
     CDS             5834..6550
                     /locus_tag="VD791_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HXV07417.1"
                     /translation="MDTVELYLEDAYTRACDTRVSRVDERGIQLERTPFYAQGGGQPG
                     DRGTLILRDGRRIEIVAALRDRDTGQVIHVPAAGVETALIGEEIEAAIDWDLRYAHMR
                     CHTCLHLLCALVPYPVTGGSVRAGSGRLDFDVPQPILDKEALTQQLNELIARNAPLST
                     ETISEQELRARPELVRTIEVGPPARQGRVRLVRIEGIDLQPCGGSHLRSTGEIGRARV
                     VKLEKKGRQNRRVEVAIEAA"
     gene            complement(6580..7026)
                     /locus_tag="VD791_05280"
     CDS             complement(6580..7026)
                     /locus_tag="VD791_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV07418.1"
                     /translation="MQINGIAHVALTVSNFEAAVPFYERLLAFLGMTCVFKGEDFLYY
                     VGGRTALGIGRCDARYREERFDQRRVGLHHVCFRARERADVDRLYPFLLSLEAKIVHP
                     PEDGPWAPGYYSVLFEDPDGIRLELNHVPGQGLLADGVAMNPVGYR"
     gene            complement(7034..7546)
                     /locus_tag="VD791_05285"
     CDS             complement(7034..7546)
                     /locus_tag="VD791_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXV07419.1"
                     /translation="MAIKVGDRLPSGTLWEFIEEETPGCSVGPNAFQVENLVKGKRIA
                     IFGLPGAFTPTCSAKHLPGYLQNADQLKSAKVDEIWCLSVNDAFVMGAWGREQGAKGK
                     VRMMADGSGLYSKALGLEMDLVARGLGVRSQRYSMLVDDGVVKTLNVEEPGKFEVSGA
                     EKMLEQAQKR"
     gene            7925..9031
                     /locus_tag="VD791_05290"
     CDS             7925..9031
                     /locus_tag="VD791_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HXV07420.1"
                     /translation="MSKIVLSPREYQEEYASMRERRNRVVQVRRFGGPEELEVVEVPL
                     PTAGRGEVRVRVLASGMEYTDVVIRRHLYPQTMRRRPPFVLGYDVVGEIDQLGDGVSG
                     LRIGERVADMTVVGSNAAYRTLRVDDVTRVPAGLDPAEAAALILSWTTAYQLLHRTAR
                     VKRGDRVLVHGAAGAVGQALLVLGRRTGLELWGTARGEHVALIRELGATPIDYQREDF
                     TQVLSGGFDAVFDGIGEDSYRRSFAALKRGGLLCVYGYSAGVQAQRRILDLLLWIARV
                     YLWRRLLSWLPGGKRVRVYSINAMRARHSAWFREDLERLFGLLATGAIRPRVAERISF
                     DEVAEAHRRLEAGGLEGKLVLCPDLSSRRDRVPA"
     gene            complement(9091..>9441)
                     /locus_tag="VD791_05295"
     CDS             complement(9091..>9441)
                     /locus_tag="VD791_05295"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HXV07421.1"
                     /translation="RHDNSFEYKLTYEDLEGIVTQAHIHFGRPGVNGGISVWLCGTST
                     NPGPAGTPTCPGPNSGEVSGTITSAEVVGPEAQGIAPGEFDELVRAMREGATYANVHT
                     DTRPGGEIRGHIDH"
BASE COUNT         1442 a         3301 c         3259 g         1439 t
ORIGIN      
        1 aaccggcgct gcggtcgagc aatagcgtgt gcttgccgca ccagccgagc ccggcctgcg
       61 cggccagctc gacctccatc accggcgcgc tgtcggtgaa gacgcgatag cggaatgcgc
      121 ccacctgcgc ctcgatgcgg gcggcgagcc gcagcagccg ccggcgcagc accttgtggt
      181 agtcgcggcc cagcgcatag cgcgagatgt aggcgagcga ggcgtccgcg agcaccgcct
      241 gcgcgtcgcg cgcgcccgcc gggaagtagt tcatgcgcag cgacagcacg cgcagcgtgc
      301 cgggcagcag cacgtcgggg cgcccgcgca gcgcggcgtg gcgctcgagg tagcccatct
      361 cgccgtgata gcagcggccg agccattcca gcaggcgcgc ctgcgcggcg tcgacgtcga
      421 taccgctcac ccccatgcgc tcgaacccca ggtcccgcgc ccagcccgcg accgagcgct
      481 tgagcgcgac caggtcgatg tccgccgagg ggctgtccac gcttgtcgct tgcatcgcgg
      541 cttcgcacga tcgattcagc gtgcagtgta aacccgcgcc cgcttcccgc gccacggcgc
      601 cgccgtcacc atccgtccta taatgcgcgt ccggttttgc gccggcggcg cggcggcccg
      661 cgcagtcgca aatcggactg gcgcagatcg gaccgggttc caccacaggg ggagtcactc
      721 gaatccatgg cgaaggcgag aaagaagatc gcggcgggtg tgatcgggct gggctcgatg
      781 ggatggggtg tggcgcgcac gctgctcaac aagggcttca cggtgtacgc ctacgacgtg
      841 cggccgcagg tgctgaagga cttcgcgcgc gcgggcggca aggcctgcac gaacccgggc
      901 gaggtcggcg ccaatgcgcc ggtggtgatc acgctggtgg tcaacgccga gcagaccgcc
      961 gaggtgctgc tgggcagcgc cggcgcggcg ccgcgcatgg cgcgcggctc ggtggtgatc
     1021 gccagcgcca cggtggcgcc ggctttcgcc gaggagctgg gcgcgcgcct caacgcgctg
     1081 ggcgtccaca tgatcgacgc gccggtgagc ggtggcgctg cccgggcggc ggcaggccag
     1141 ctctccatca tggcctccgg cgcgcgcgag gcgttcctgc gcgcacggcc cgtgctcgaa
     1201 gccgtcgccg cgaaggtcta tcgcctgggc gatgcgcccg gcatgggctc gaaggtcaag
     1261 atggtgaacc agctgctggc cggcgtgcac atcgccgctt cggccgaggc gatggcgctc
     1321 ggcatccgcg ccggcgtcga tcccaacgtg ctgtacgagg tcatctccaa cagcgcgggc
     1381 tcgtcctgga tgttccagaa ccgcgtgccg cacatcctgg ccggggacta cacgccgctg
     1441 tcggcggtga acatcttcgt caaggatctg ggcatcgtac tcgactcggc gcgcaaggcg
     1501 acctttccgc tgccgctcac cgccaccgcg caccagatgt tcctcatggc cggcgcccac
     1561 gggcacgggc aggaagacga ctcggcggtg gtcaaggtgt tccaagccgt ctccggcatc
     1621 ccgctgccgg agaagaagcg cgcgcccagg ccgcgcaccg cggcgcgccg caagtgagcg
     1681 tcaaatcgac tgcttgccgc tttggcccag gcggtagcgg atcagcgcga tgctctgctc
     1741 cacgccttcc aggttggggt tgatgtcgag cgcgcgctcg aaatactcca gcgcgcgctc
     1801 cagctcgccg agctgcacgt agatcagccc gtagcccgac agcgcgccga agtggctggg
     1861 attgcgctcg atcacctggt cgcagtcctt cagcgactgg tcgtagtcgc cgatcagata
     1921 gtaggcggtc gcgcgcttgt tccagccctc ggcgaaatcg ggacgcaggt cgatcacgcg
     1981 cgtgaatgcg tcgaccgcct ggcccatctg cccgtcctcc atgtggcgga tgccgcgctg
     2041 gagcagaccg tcgacctcgg ggtctccgga gcgtgcccac acctgccaca cggcgcgctc
     2101 ggccagcgcg cgtacgtcgg cgtcctcgtc gcgcaggtgc ccgatgagcg cccccgcatc
     2161 cgccatcccg ccggtgcggc ccagctcggt gaccgcttcg cgccgggcgg tcgcatccgg
     2221 gctgtcgagc gccatcaggg cctgctcgcg cgtgagctct ccggcggccg cgcctgcggc
     2281 gagcaaaagg cagagtagcc aggcgcggga gacagctcgg gaaacggccc gggaaagaac
     2341 ccaggagagc gcgcggcgca gcagcatggc gtactcctca cggcgcccgc cgtcggtggg
     2401 cgccggagtc gaggtgacga tgccgtgatt ctaacgccgc gagacggccg cgtgctgccc
     2461 gcgctcccgc atccccggtg caagctgtag agtagcggct ggaaatcgaa ccgggagaag
     2521 ccgatgaacg cgccacgcga gcccgccccc gcgcaagccc tgccgccccc ggcgcaggcc
     2581 ggcatgcgcc tggagcaggt ggacacccct gccctgctgc tggatctgga cgccttcgag
     2641 cgcaatctgc gccggctgca cgcctccctg ggcgatgcga aggtgcgtgt gcgcccgcac
     2701 gccaagtcgc acaagtgccc gcagatcgcg ctgcgccaga tcgcgctcgg cgccgtgggc
     2761 gtgtgctgcc agaaggtgag cgaagccgag gcgctggtgc gcgacggcgt cggcgacgtg
     2821 ctgatcgcca acgagatcgt cggagcgcag aagctcgcgc gcctgaccgc gctggcgcgc
     2881 agcgccaaga tcggcgtgtg cgtggacgat atcgcgaacg tggccgatct cgatcgcgcc
     2941 gcacgagagg ccggcgcgcg gctgaatgtg tacgtcgaga tgaacgtcgg cgccaaccgc
     3001 tgcggtgtgg agccgggtga gccggcgctg cgcctggcac aggcagtcgc acgcgcgggt
     3061 gcattgcgct tcgccggcgt tcaggcgtac cagggcgccg cgcagcatct gcgcagcgcc
     3121 gccgagcgca gcgccgcgat cgccaaggcg gtggagcagg tgcgggaaac cgtggcgctg
     3181 ctcgaggcca acggcttgcg cgccgagatc gtcaccggcg caggcaccgg cacttacctg
     3241 ctggaggcgg cgagccgagt ctacaacgag atccagcccg gctcctacgt gttcatggac
     3301 gccgactacg gccgcaacct gggcgaggac ggccagcccg tgcacgagtt cgcacaaagc
     3361 ctgttcatcc tggccacggt catgagccgg cccaccccgc agcgcgcggt ggtggacgcg
     3421 gggctcaagg cgcacagcgt ggactccggc atgccggcgg tggtcgggat cgcgggcgcg
     3481 cgctacgtcc gcgcgtccga cgagcacggt gtgatcgagc tgggcggatc cggcacggcc
     3541 ggtctcggac agaagatccg cctgatcccc ggccactgcg atcccaccgt caatctctac
     3601 gactggctgg tgtgctatcg cggcgaacgc gtcgaggaca tctggccgat ctccgcgcgc
     3661 ggcgctttct attgaccgag gcaacgtcat gcccgcaccc cgctacctga gcttcgacac
     3721 cctgagcctg cacgccggcc agcggccgga cccggccacc ggcgcgcggg ccacgcccat
     3781 ttaccagacc tcgtcgtacg tgttccgcga cacggaccac gcggcggcgc tgttcaacat
     3841 ggagcgcgcc gggcatgtct attcgcgcat ctccaacccg accaacgccg tgctggagga
     3901 gcgcattgcc gcgctcgaag gcggcatcgg cggcatcgcc acggcgagcg gccaggccgc
     3961 cctgcacctg gccgtggcca ccatcgccgg cgccggctcg cacatcgtgt cgagccgctc
     4021 gctgtacggc ggctcgcaca atctgctgca ctacacgctg ccgcggttcg gcatcgagac
     4081 gaccttcgtc gacccgcgcg acctcgatca atggcgcacg gcgatgcgcc cgaacacgcg
     4141 tctgctgttc ggcgagacgc tgggcaaccc cggcctggat gtgctcgacg tgccggccgt
     4201 cgcgcagatc gcgcacgatg cgaagatccc cctgctggtc gacgccacct tcaccacgcc
     4261 gtacctgatg cgccccttcg agctgggcgc ggacctgctg tatcactccg ccaccaagtt
     4321 cctgggcggg cacggcatcg tcatcggcgg tctgctggtg gacagcggcg cgttcgactg
     4381 ggaggcctcc ggcaagttcc ccgagctgac cgaaccgtac agcggcttcc atgggatgga
     4441 cttccaggag gagtccacgg tggcggcttt tctgctgcgc gcgcgccgcg agggcctgcg
     4501 cgacttcggc gcgtgcatgg cgcctaccac ggcgttccac atcctgcagg gcatcgagac
     4561 gctcggcctg cgcatggcgc ggcacgtgga caacacgcgc aaggtagtgc agttcctggt
     4621 cgggcacccg gcggtggagt cggtgggcta cccggagctg cccacgcacc ccgaccatga
     4681 gatcgcgaag cggctcctgc cgcgcggttg cggcgccgtg ttcccgttca gcatccgcgg
     4741 cggccgcccc gccggccgca agttcatcga gtcgctgcgc gtgttttcac acctggccaa
     4801 cgtcggcgac gccaaatccc tggtgatcca ccccgccacc accacgcact tccgcatgga
     4861 cgacgccgcg ctcgccgccg ccggcatcgg ccccggaacg atccgcctgt cgatcggcct
     4921 ggaggatccg caggacctga tcgacgatct ggcgggggcg ctgcgcgctg cgcagaaggt
     4981 ctgaagcccg cgatcaccac agagttacac agagggacac cgaggaagcc gtggatctga
     5041 gagtcgacgg aaagcaggca tatgcctaca ccggcgggcg gccgttcgat ggcgcgcggc
     5101 ccgcggtggt gttcatccac ggcgccgagc acgaccactg cgtgtgggtg ctgcagagcc
     5161 gctacctcgc gcaccatggg cgcagcgtgc tggcggtcga tctgcccggc cacgggcgca
     5221 cggcgggccc accgctggcg agcgtcgagg cgctggcgga ctggatcgtg gcgctgctga
     5281 acgccgcggg cgcccccaag gcggccctgg tgggccacag catgggatcg ctgatcgcag
     5341 tggagtgcgc ggcgcgtcac cccgaacgca tctccaagat cgccctggtc ggcaccgctt
     5401 ttcccatgcg cgtcgcgcca gagctgctcg aggccacacg cagcgccgag ccgctggcgc
     5461 aggacatggt caacatctgg tcccactcga gctacgccca gtatccgagc aacccggggc
     5521 ccgggttctg ggtgatgggc gagaacctgc gcctgatgca gcgccagaag ccgggcgtca
     5581 tgcacgtcga tttcgccgcc tgcaacgcct acgccaacgg cgtccaggcc gcggcgcagg
     5641 tcgtctgccc cgctctgctg gtgctgggca agcgcgacat catgacgccg ccgcgtgcga
     5701 cggccgagct caccgccgcg ctgcgcgacg cgcgcgtcgt cgagatcccc ggcaccggcc
     5761 atgccctgat ggccgagaag ccggacgaag tgctggacgc cctcgccgcc tttctctagc
     5821 gggcagacgc gagatggaca cggtcgagct gtatctcgag gacgcttata cccgagcctg
     5881 cgacacgcgc gtgagccgcg tcgacgagcg cggcatccag ctcgagcgca cgccgttcta
     5941 tgcacagggc ggcggccagc cgggcgaccg cggcacgctc atcctgcgcg acgggcgccg
     6001 catcgagatc gtcgccgcct tgcgcgatcg cgacacggga caggtgatcc acgtgccggc
     6061 tgcgggagtc gagaccgcct tgatcggcga ggagatcgaa gcagcgatag attgggatct
     6121 gcgctatgcg cacatgcgtt gccacacctg cctgcatctg ctgtgcgcgc tggtccccta
     6181 cccggtcacc ggcggcagcg tgcgcgccgg atcgggccga ctggacttcg atgtcccgca
     6241 gccgatcctg gacaaggagg cgctcacgca gcagctcaac gagctgatcg cccgtaacgc
     6301 cccgttgtcg acggaaacca tcagcgagca ggagctgcgg gcgcggcccg agctggtgcg
     6361 caccatcgag gtcggcccgc cggcgcgtca gggaagggtg cgcctggtgc gcatcgaagg
     6421 catcgacctg caaccctgcg gcggcagcca cctgcgcagc accggcgaga tcggccgtgc
     6481 ccgggtcgtg aagctggaga agaaaggccg gcagaaccgc cgcgtcgaag tcgccatcga
     6541 ggcggcttga gcgcgaaggg cgacacggcc ccctgcggcc tagcgatagc ccaccggatt
     6601 catggcgacg ccgtccgcga gcagcccctg tccgggcacg tggttcagct ccagccgaat
     6661 gccgtcggga tcctcgaaca gcaccgagta gtagcccggc gcccagggac cgtcctcggg
     6721 cggatgcacg atcttcgcct cgagcgagag caggaacggg tacaggcggt cgacatccgc
     6781 gcgctcgcgc gcgcggaagc agacgtggtg cagccccact ctgcgctggt cgaagcgctc
     6841 ctcgcggtag cgtgcgtcgc aacggccgat gccgagcgca gtgcgcccgc cgacgtagta
     6901 caggaagtcc tcgcccttga atacgcaggt catgccgagg aatgcgagca gccgctcgta
     6961 gaacggaacg gccgcctcga agttgctgac cgtgagggcg acatgcgcga tgccgttgat
     7021 ctgcattcgg agatcatctc ttctgcgcct gctcgagcat cttctcggcg ccgctcacct
     7081 cgaacttgcc gggctcttcg acgttcagcg tcttgaccac accgtcgtcc accagcatgg
     7141 agtagcgctg cgagcgcacg cccagcccgc gggcgaccag gtccatctcc agcccgaggg
     7201 ccttggagta cagaccgctg ccgtcggcca tcatgcgcac cttgcccttg gcgccctgct
     7261 cgcggcccca cgcgcccatg acgaaggcgt cgttcaccga caggcaccag atctcgtcca
     7321 ccttcgccga cttgagctgg tccgcgttct gcagataacc gggaaggtgc ttggccgagc
     7381 aggtgggggt gaacgcccct ggcaatccga agatggcgat gcgcttgccc ttgaccaggt
     7441 tctccacctg gaaggcgttg ggcccgacgc tgcaacccgg ggtctcctcc tcgatgaact
     7501 cccacagggt gccggacggc aggcggtctc cgactttgat ggccatggcg gtctcctcgg
     7561 ttgatgtacg acctcggatt ctatcaagcc ggctcgatcg ccatcgcgcg cctcagcgag
     7621 ccccggcagg gcagcacctc gttgctccag aagcatcgag tggaagccac gggtggccgg
     7681 gcgttacccc ggctgctatg tccgcatcgc cgtgtcgggc gaattcttcc gaccgtacgc
     7741 tggcgagcga tgcgcacctt cacacctgcg gcgcatcgcg gcgctaaccg cgcaggcacc
     7801 cgtggtgtga tcattgtgca ggattcgcgc cggtcccaca ccgtgcggcc gtgtccccgg
     7861 tcatcgcgcc actgcttgac ttggatcaag cacgtgttcg ctcacgagcc tctaatggcc
     7921 gagtgtgtcg aagatcgtgc tctctcctcg ggagtaccag gaggaatacg cgtccatgag
     7981 agagcggcgc aaccgggttg ttcaagttag gcgcttcggc ggtcccgaag agctggaggt
     8041 ggtcgaggtt ccactgccga cggccggccg gggcgaggtg cgggtgcgcg tgcttgcctc
     8101 gggcatggag tacaccgacg tggtgatccg gcgccacctg tacccgcaaa cgatgcgccg
     8161 ccggccgcca ttcgtgctgg gctatgacgt cgtcggggaa atcgatcagc ttggcgatgg
     8221 tgtgagtggc cttcggatcg gcgagcgcgt ggccgacatg accgtggtcg ggtcgaacgc
     8281 cgcctatcgc acgctgcggg tcgacgacgt gacccgcgtg ccggcgggcc tcgacccggc
     8341 agaggcggcc gcgctgatct tgagctggac gaccgcatac cagctccttc accgcacggc
     8401 ccgggtcaag cggggcgacc gcgtgctcgt gcatggggcc gccggcgccg tcggtcaggc
     8461 gctcctcgta ctcggcaggc ggaccggcct cgagctgtgg ggcaccgcgc gcggcgagca
     8521 cgtggcgctg atccgcgaac tcggcgccac cccgatcgac tatcaacgcg aagatttcac
     8581 gcaagtcctg tcgggtggat tcgacgccgt cttcgacggc atcggcgagg acagctatcg
     8641 ccgatcgttc gcagcgctca agcgcggcgg cctgctctgc gtgtacggtt actcggcggg
     8701 cgtccaggcc cagcgtcgca tcctcgacct cttgctgtgg attgcgcgcg tgtatctatg
     8761 gaggcgattg ctgagttggt tgcccggcgg caagcgagtc cgcgtctact cgatcaatgc
     8821 gatgcgggcg cgacattccg cctggttccg ggaggacctc gagcggctct tcggcctgct
     8881 ggcgactggc gccatccggc cgcgggtcgc cgagcgcatc tctttcgatg aggtcgccga
     8941 ggcacaccgc cgtctcgagg cgggcggtct cgagggcaag ctcgtcctgt gcccggacct
     9001 ctcgtcgcga cgcgaccgcg tgcccgcata ggaaattcaa gcgctatcag ggattctagc
     9061 cctctcgacc gccatgcccg tcgcggcgga tcagtggtca atgtgcccgc gtatctcgcc
     9121 gcccgggcgt gtgtccgtgt gcacgttcgc gtacgtcgca ccttcacgca tcgccctgac
     9181 cagttcgtcg aactcgcctg gcgcgatgcc ttgcgcttcc gggccgacca cttcggcaga
     9241 ggtgatggtg ccggagacct cgcccgagtt cggccccggg caggtcggcg tgcccgcggg
     9301 acccggattg gtactggtgc cgcacagcca gacgctgatg ccgccgttga caccggggcg
     9361 cccgaaatgg atgtgggcct gcgtcacgat cccctcgaga tcttcatagg tcagcttgta
     9421 ttcgaacgag ttgtcgtggc g
//
LOCUS       DASPGE010000168         5982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_514880, whole genome shotgun sequence.
ACCESSION   DASPGE010000168 DASPGE010000000
VERSION     DASPGE010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514880"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VD791_05300"
     CDS             complement(<1..494)
                     /locus_tag="VD791_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HXV07422.1"
                     /translation="MINRMLVYLGRHVDSYLLAAVGALLVVGLITLYSASGESFARLG
                     NQTINIVVALALMWLAANIPPHYLQRLALPVYAVGLLLLIAVALFGEIVNGARRWLDL
                     GITRIQPSELMKIAVPMALAWYFDRYEAILKLRDYLIAAVMMAVPVLLVVKQPDLGTA
                     LLICA"
     gene            complement(491..2440)
                     /gene="mrdA"
                     /locus_tag="VD791_05305"
     CDS             complement(491..2440)
                     /gene="mrdA"
                     /locus_tag="VD791_05305"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXV07423.1"
                     /translation="MRLFRRFSARKETAPIVLGGARRAELRDHQKDLHNFQLRLAVSA
                     ALVLAGFCVLLVRLFYLQVLQHDYYHTLAENNRISIVPIIPNRGLILDRNGAVLAHNY
                     AAYTLEITPSKVVDLDGLIDELAQLVEVAPRDRKRFRKLLDEGHEFASLPIRTRLNDE
                     EVARFAANKYRFQGVDIRARLFRHYPNGEIGSHVLGYIGRLTKADVERLQGEGIAANY
                     TGSDFIGKAGVEARYERQLHGTTGFEEVETDAGGRAVRTLRSTLPVSGNNLMLSLDAR
                     LQDVAERVFGERRGALIAIEPATGGVLALVSKPGFDPNLFVDGIDPQNWEQLNSSPDH
                     PLNNRALQGVYPPGSTFKPFMAIAALELKKRSPHYTIADPGYFTLGGAHRWRDWKYGG
                     HGMVDLHKSVVVSCDTYYYGLAHDLGIDSIHQFIGQFGFGKRTGIDIDGEAGGLLPSQ
                     EWKMRRFKQKWFPGDTISVGIGQGYNLATPMQLAHATSVLANRGVVFRPHVVRQIQNS
                     VTNALTSIEVEPIGKIALNPVNLERVTNAMVDVMRPGGTAAWAGSGAQYRIAGKTGTA
                     QVIGLKQHERYDERRIAERHRDHALFIAYAPADKPNIALAVLVENGGHGSSTAAPIAR
                     KVFDFYLLGKEPEMKPEPPPPEGEE"
     gene            complement(2437..2976)
                     /gene="mreD"
                     /locus_tag="VD791_05310"
     CDS             complement(2437..2976)
                     /gene="mreD"
                     /locus_tag="VD791_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HXV07424.1"
                     /translation="MMRLQELEAERAQTAELLRPVRGRYIAGTLLVALLFNLLLLPDF
                     LHTLRPDFVGLTLIYWVVYHPRRVGFLPAWLMGLAMDVVDGSLFGQHALAYAGLMYLA
                     ILLHRRIVMFGMRQQVVHVLAILAGSQAVMLLVRLTAGSDFPGPLYFAPSIVGAILWP
                     GMFNLIRLPLRPRADPDAV"
     gene            complement(2973..3863)
                     /gene="mreC"
                     /locus_tag="VD791_05315"
     CDS             complement(2973..3863)
                     /gene="mreC"
                     /locus_tag="VD791_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HXV07425.1"
                     /translation="MEYTPPPFFKRGPSLLTRFAFFSMASIVLLVADARFNYLQSMRQ
                     VVAVAVYPLQQLSSVPSRMTRWIGEVAVSHSHLRQENEQLRQRGLLDAAALQNQHALA
                     AENGHLRQLLAMRERVPRELTAAEILSAPRDPFTRRVVVDKGSQHGVRAGAAVVDRVG
                     VVGQVTRVFPWLSEVTLITDKEQAVPIQVLRSGLRGVTFGIGDDSTLELRFMPVNADI
                     QNGDVLVTSGIDGTYPPGLPVAVVSKIERNTAYAFARITCVPAAGVNSYSQLLIVGMD
                     RQQPGRPADGSGAPARKAGP"
     gene            complement(3977..5020)
                     /locus_tag="VD791_05320"
     CDS             complement(3977..5020)
                     /locus_tag="VD791_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HXV07426.1"
                     /translation="MIGFLGGYFSNDLAIDLGTANTLIYVRGKGIVLNEPSVVAIRQE
                     GGPNGKKVIQEVGLAAKQMLGRTPGNIIAIRPMKDGVIADFTVTEQMLKQFIKKVHDN
                     RLFSPSPRIVICVPCGSTQVERRAIRESAYGAGARKVELIEEPMAAAIGAGLPVDEAT
                     GSMVVDIGGGTTEVGVISLGGIVYAGSVRVGGDKFDEAIINYIRRNYGMLIGETTAEE
                     IKKEIGSAFPGSEVREKEVKGRNLAEGIPRSFTISSNEILEALTEPLNSIVSAVKSAL
                     EQTPPELGADIAEKGMVLTGGGALLRDIDRLLMEETGLPVIVAEDPLTCVVRGSGKAL
                     ERMDKVGTIFTSD"
     gene            5265..5552
                     /gene="gatC"
                     /locus_tag="VD791_05325"
     CDS             5265..5552
                     /gene="gatC"
                     /locus_tag="VD791_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HXV07427.1"
                     /translation="MPLSPADVQRIARLARIELADGELAQALAQLNGIFALIAELQAV
                     DTEGIEPMSHAQDLVLRLRPDRVAQADQRAEFQAIAPAVENGLYLVPKVIE"
     gene            5609..>5982
                     /locus_tag="VD791_05330"
     CDS             5609..>5982
                     /locus_tag="VD791_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXV07428.1"
                     /translation="MHNATLAELSQALRARRISSVELTRLFLERAQRLNGTLNAFITL
                     DPERSLALAAAADRRIAAGQAGPLTGIPIAHKDIFCCKGWLTSCGSRMLSNFVAPYDA
                     HVVERLHHAGLVTLGKTNMDEFA"
BASE COUNT          958 a         2072 c         2015 g          937 t
ORIGIN      
        1 gcgcagatca gcagcgcggt gcccaggtcc ggctgcttga ccaccagcag caccggaacc
       61 gccatcatca ccgccgcgat caggtaatcg cgcagcttga ggatcgcctc gtagcggtcg
      121 aagtaccacg ccagggccat cggcaccgcg atcttcatca gctcggaggg ctggatccgg
      181 gtgataccca ggtcgagcca gcggcgggcg ccgttgacga tctcgccgaa cagcgccacc
      241 gcgatcagca gcagcaggcc gaccgcgtac acgggcaacg ccagccgctg caggtagtgc
      301 ggcgggatgt tggccgccag ccacatcaag gccagcgcca cgacgatgtt gatcgtctgg
      361 ttgccgaggc gggcgaagct ctcgcccgag gcgctgtaca gcgtgatcag cccgaccacc
      421 agcagcgcgc cgaccgcggc cagtagatag ctgtccacgt gccggcccag atagaccagc
      481 atccggttga tcattcctcg ccctccggtg gcggcggctc cggtttcatc tccggctcct
      541 tgcccagcag gtagaagtcg aacaccttgc gggcgatggg cgcggcggtg gaggaaccgt
      601 gtccgccgtt ctccaccagc acggcgagcg cgatgttcgg cttgtcggcg ggcgcgtagg
      661 cgatgaacag cgcgtgatcg cggtggcgct cggcgatgcg ccgctcgtcg tacctctcgt
      721 gctgcttcag cccgatcacc tgtgccgtcc cggtcttgcc ggcgatgcgg tactgcgcgc
      781 cggagccggc ccaggcggcg gtgccgccgg gccgcatcac gtccaccatg gcgttggtca
      841 cgcgctccag gttcacgggg ttgagcgcga tcttgccgat cggctccacc tcgatgctgg
      901 tgagcgcatt ggtcacgctg ttctggatct ggcgcacgac atgcgggcgg aagacgacgc
      961 cgcggttggc gagcaccgag gtggcgtgcg ccagctgcat cggcgtggcc agattgtaac
     1021 cctggccgat gcccacggag atggtatcgc ccgggaacca cttctgcttg aagcgccgca
     1081 tcttccactc ctgcgagggc agcagcccgc cggcctcacc gtcgatgtcg atgccggtgc
     1141 gcttgccgaa gccgaactgg ccgatgaact gatggatgct gtcgatgccc agatcatggg
     1201 ccaggccata gtagtaggtg tcgcaggaca ccactacgga cttgtgcaga tccaccatgc
     1261 cgtggccccc gtacttccag tcgcgccagc ggtgcgcgcc gcccagagtg aaatagccgg
     1321 gatcggcgat ggtgtagtgt ggactgcgct tcttcagctc gagggcggca atcgccatga
     1381 acggcttgaa cgtggaaccg ggcgggtaca cgccctgcag cgcgcggttg ttgagcgggt
     1441 gatcggggga gctgttgagc tgctcccagt tctgcgggtc gatgccgtcg acgaacaggt
     1501 tcgggtcgaa gccgggcttg ctcaccagcg ccagcacgcc gccggtggcc ggttcgatgg
     1561 cgatcagcgc gccgcgccgc tcgccgaaca cgcgctcggc gacgtcctgc agccgggcgt
     1621 cgagcgacag catcaggttg ttgcccgaca ccggcagggt gctgcgcagc gtgcgcaccg
     1681 cgcggccgcc ggcgtcggtc tccacctcct cgaagccggt ggtgccgtgc agctggcgct
     1741 cgtagcgcgc ttccacaccg gccttgccga tgaaatccga cccggtgtaa ttggcggcga
     1801 tgccctcgcc ctgcagccgc tccacgtccg ccttggtgag ccgcccgatg tagcccagca
     1861 cgtgcgaccc gatctcgccg ttggggtagt ggcggaacag ccgggcgcgg atatccacgc
     1921 cctggaagcg atacttgttg gcggcgaagc gtgccacctc ctcgtcgttg aggcgcgtgc
     1981 ggatcggcag gctggcgaac tcgtgcccct cgtcgagcag cttgcgaaaa cgcttgcggt
     2041 cgcgcggcgc cacctccacc agctgcgcca gctcgtcgat caggccgtcc agatcgacca
     2101 ccttgctggg cgtgatctcg agcgtgtagg ccgcatagtt gtgcgccagg acggcgccgt
     2161 tgcggtccag gatcagcccg cggttgggga tgatcgggac gatcgagatg cggttgttct
     2221 cggccagcgt gtgatagtag tcgtgctgca gcacctgcag gtagaacagc cgcaccagca
     2281 gtacgcagaa gccggcgagc accagcgccg cggacaccgc cagccgcagc tggaagttgt
     2341 gcaggtcctt ttgatggtcg cgcagctccg cgcggcgcgc tccgcccagt acgatcggcg
     2401 cggtctcctt gcgcgcggag aagcggcgga acagcctcat acggcgtccg gatccgcgcg
     2461 cggccgcagc ggcaggcgga tcaagttgaa catccccggc cacaggatgg cgccgacgat
     2521 gctcggcgcg aagtacagcg gccccgggaa gtcggaaccg gcggtgagcc gcaccagcag
     2581 catcaccgcc tgcgagcccg cgaggatcgc cagcacgtgc accacctgct gacgcatgcc
     2641 gaacatgacg atgcgccggt gtagcaggat cgccaggtac atgagcccgg catacgccag
     2701 cgcgtgctgc ccgaacaggc tgccgtcgac cacgtccatc gccagtccca tcagccaagc
     2761 cggcaggaac ccgacccggc gcgggtggta gaccacccag tagatcagcg tcaggccgac
     2821 gaagtcggga cgcagggtat gcaggaaatc cggcagcaac agcaggttga acaacaacgc
     2881 caccagcagc gtgccggcga tgtaccggcc gcgcaccgga cgcaggagct cggcggtctg
     2941 cgcgcgctcg gcttcgagct cctgcagccg catcatggcc cggccttgcg cgcgggcgcg
     3001 ccggacccgt ccgccgggcg acccggctgc tgccgatcca tgcccacgat cagcagctgg
     3061 ctgtagctgt tgaccccggc ggccggcacg caggtgatgc gggcgaaggc gtaggcggtg
     3121 ttgcgctcga tcttggacac caccgccacc ggcaggcccg gcgggtaggt gccgtcgatg
     3181 cccgaggtca ccagcacgtc gccgttctgg atgtcggcgt tgaccggcat gaagcgcagc
     3241 tcgagcgtgc tgtcgtcgcc gatgccgaag gtgaccccgc gcagcccgct gcgcagcacc
     3301 tggatcggca ccgcctgctc cttgtcggtg atcagggtga cctcggacag ccaggggaag
     3361 acgcgcgtga cctgccccac cacgccgacg cgatccacca ccgccgcgcc cgcgcgcacg
     3421 ccatgctggc tgcccttgtc caccaccacc cgccgggtga agggatcgcg cggcgcggac
     3481 aggatctcgg cggcggtcag ctcgcgcggt acgcgctcgc gcatggcgag cagctggcgc
     3541 aaatgcccgt tctccgcagc cagggcgtgc tggttctgca aggcggccgc atccagcagc
     3601 ccgcgctgcc gcagctgctc gttctcctgg cgcaggtggc tgtggctgac ggcgacctcg
     3661 ccgatccagc gcgtcattcg gctcggcacc gaggacagct gctgcagcgg atacaccgcc
     3721 accgccacca cctggcgcat ggattgcagg tagttgaagc gcgcatcggc caccaacagc
     3781 acgatggagg ccatcgagaa gaaggcgaac cgcgtgagca ggctggggcc gcgcttgaag
     3841 aacggaggcg gggtgtattc cattgcggct cagggttcac cgccgagcgc gtacccgccg
     3901 ctcccggcga cgggggcctg cgccgggagg ctgcggcggt tagcggtgct cgagcggggc
     3961 gccggcgagg gattcctcag tcgctggtga agatggtccc gaccttgtcc atgcgctcca
     4021 gcgccttgcc ggagccgcgc accacgcagg tcagcggatc ttcggcgacg atcaccggca
     4081 acccggtctc ttccatcagc agccggtcga tatcgcgcag cagcgcgccg ccgccggtga
     4141 gcaccatgcc cttctcggcg atgtcggcgc ccagctccgg cggggtctgc tccagagcgg
     4201 acttgaccgc gctcacgatg ctgttgagcg gctcggtgag cgcctccagg atctcgttgc
     4261 tggagatggt gaagctgcgc gggatgccct cggccaggtt gcggcccttg acttccttct
     4321 cgcgcacctc gctgccggga aaggccgaac cgatctcctt cttgatttcc tcggcggtgg
     4381 tctcgccgat cagcatgccg taattgcgcc ggatgtagtt gatgatggct tcgtcgaact
     4441 tgtcgccgcc cacgcgcacc gagccggcgt acacgatgcc gcccagcgag atgacgccca
     4501 cctcggtggt gccgccgccg atgtccacca ccatcgagcc ggtggcctcg tccaccggca
     4561 gcccggcgcc gatggcggcc gccatcggct cctcgatcag ctccaccttg cgcgcgccgg
     4621 cgccgtaggc cgactcgcgg atcgcacgcc gctccacctg agtcgagccg cagggcacgc
     4681 agatcacgat gcgcgggctg ggcgagaaca ggcggttgtc gtggaccttc ttgatgaact
     4741 gcttgagcat ctgctcggtg accgtgaagt ccgcgatcac cccgtccttc atcggacgga
     4801 tggcgatgat gttgccgggg gtgcggccga gcatctgctt ggcggcgagg ccgacctcct
     4861 ggatcacttt cttgccattc ggtccgccct cctggcggat ggcgaccacg gacggttcgt
     4921 tgagtacgat gcccttgccg cgcacgtaga tcagcgtgtt ggccgtgccg aggtcgatgg
     4981 cgaggtcgtt ggaaaaatag ccgccaagga atccgatcat gcacctcccg gttgagattc
     5041 gaacgtaggt ctcggacgga tcgagactaa attctgaaaa aagctataat aacgcacctg
     5101 gcgagccgga ccaaagaagc ccgcgcccgc gcgccgggcc cgccgctacc gacgaatgcg
     5161 ggcgcccgtc tgctgcgcgc gacgcccccg gcgcaacaca acatcgtcac caacgacggc
     5221 gggcttgagt cccgtcgcaa ccgcggcgca tttctccccg tcccatgccc ctgtcccctg
     5281 ccgacgtcca gcgcatcgca cggctggcgc gcatcgagct ggccgacggc gagctcgcgc
     5341 aggccctggc tcagctcaac gggatcttcg ccctgatcgc ggaactgcaa gcggtggaca
     5401 ccgagggcat cgaacccatg tcccacgccc aggacctggt gctgcgcctg cgcccggacc
     5461 gggttgccca ggccgaccag cgcgccgagt tccaggccat cgcacccgca gtggagaacg
     5521 gtctgtacct ggttccgaag gtcatcgaat agccggggtc atcgagtaaa ccggaccggg
     5581 gcggacccgc cgcccgcgcg ccttgccgat gcacaacgcc acccttgccg agctgtccca
     5641 ggccctgcgc gcgcgccgca tctccagcgt cgagctgacg cgcctgttcc tcgagcgggc
     5701 gcagcggctc aacggcacgc tgaatgcctt catcaccctg gatccggaac gcagcctggc
     5761 gctggcggcg gcagccgacc gccgcatcgc cgccgggcaa gccggccccc tgaccggcat
     5821 tcccatcgcg cacaaggaca tcttctgctg caaggggtgg ttgaccagct gcggctcgcg
     5881 catgctgtcg aatttcgtgg cgccttacga cgcgcacgtg gtcgagcggc tgcaccacgc
     5941 gggcctggtc acgctgggca agaccaacat ggacgagttc gc
//
LOCUS       DASPGE010000169         7398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_866122, whole genome shotgun sequence.
ACCESSION   DASPGE010000169 DASPGE010000000
VERSION     DASPGE010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866122"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VD791_05335"
     CDS             <1..283
                     /locus_tag="VD791_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07429.1"
                     /translation="FSRFTDDYDAVVTPPAAGEAPASLDTTGDPSFCTIWTLIGVPAI
                     VIPTGLGSHGLPLAMQIIGNQGESNHLLATSMWCERQLPFRGLIARELL"
     gene            complement(314..1507)
                     /gene="pncB"
                     /locus_tag="VD791_05340"
     CDS             complement(314..1507)
                     /gene="pncB"
                     /locus_tag="VD791_05340"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HXV07430.1"
                     /translation="MIIQSLLDTDLYKFTMMQVVLHHFPGAMVEYRFQCRTPEVDLTA
                     HAGEIRRQIRHLCSLRFQREELDYLRGLRFMKGDFVDLLRLFQLDEGFIHVEESPQVS
                     GGLDIAIRGPWLHTILFEVPVLAIVSETYFRATQPDPDYAEGTRRLEQKIGTITGYPD
                     AGFKISDFGTRRRFSRQWQQTVVRTLKARMNERFVGTSNVWLARSEDLIPLGTMAHEY
                     LQACQAVGPRLRDSQKFAFDTWAKEYRGDLGIALTDICGVDAFLHDFDLFFCKLFDGV
                     RHDSGDPFEWGEKLLAHYRAMRVDARTKTFVFSDSLNIPLCVELYRRFHTRCKPAFGL
                     GTNLTNDLGYRPLQIVIKMTRCNGQPVAKISDTPEKTMDYDPSYVAYLREVFQVKGPV
                     PESVE"
     gene            complement(1504..1941)
                     /gene="arsN2"
                     /locus_tag="VD791_05345"
     CDS             complement(1504..1941)
                     /gene="arsN2"
                     /locus_tag="VD791_05345"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HXV07431.1"
                     /translation="MQFGRVSPDEHQQVLRLLAQCGLPVEDVDPDLLRRFIRARGAAG
                     LAGVVGLQLLDHAALLRSLAVAPDQRGLGVGSRLCDEAEALARAGKVTDLYLLTTTAA
                     DWFAARGYVRVERSALPQAVQETSEFRHLCPASAVAMHKHLQR"
     gene            2077..6048
                     /gene="hrpA"
                     /locus_tag="VD791_05350"
     CDS             2077..6048
                     /gene="hrpA"
                     /locus_tag="VD791_05350"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HXV07432.1"
                     /translation="MNPQREPQSTASEAPDLRALQRLVAHTMLRDRHRLRSALARAFD
                     AERAQRLSATETLALAADIERSAALARERRARLPRVRYPEELPVSERKGEIATALAAH
                     QVVIVSGETGSGKTTQLPKVCLELERGVAGMIAHTQPRRIAARTVAARIAQELGSPLG
                     QDVGYRVRFSDRVSERNFLRVMTDGILLAETQTDRMLAAYDTVIIDEAHERSLNIDFL
                     LGYVKRLLPRRPELKLIVTSATIDAQRFSAHFDGAPVIEVSGRLYPVDVRYRPLAAQG
                     EDEQEIDLPEAIADAVDQVARLSAGGDVLVFLPGEREIRETAEVLRKHVFRGAARAQH
                     VEILPLYARLSAQEQERVFQPAGMRRIVLATNVAETSLTVPGIKYVVDSGLARVNRYS
                     YRNKHEMLQVEKISRASADQRAGRCGRVMSGVCVRLYAEEDYQARPAFTDPEILRSSL
                     ASVILRMKALKLGEVEDFPFVEPPSARAIADGYSLLVELGAVDAERRLTPVGARLAEL
                     PIDPGLARMIVAARDENCLTEMLVVSAGLAIQDVRERPLDKAEAADTAHAPFADERSE
                     FLGLLKLWAFFEDALRHKKSNRRLAQLCRDSFLSYVRLREWRDLHGQLHAQVSELGWR
                     FNQAPATHEQLHRAILTGLLGNVGAKSEEAGVYLGARGIKFAIFPGSPLRKKGPPWLM
                     AAELIETTRLYARTVAAIDTQWLERVGAHLTKTTWHDPHWEKSGGQAYAYERVTLYGL
                     VVVSRRRVAFGPVDPKAAREIFIREALVHGQLATHGGFLRHNRALREEIEALEHKSRR
                     HDVLVDDEAVFAIFDAILPQDVWSAQQFERWRREAERASPRLLFLTREQLMRHAAQEI
                     TEQRFPAAIEIGGVAYDLSYRFEPGHPLDGVTLSVPLHLLNQLDERRCEWLVPGLLRD
                     KVSHLLRGLPKNLRRNFVPAPQYVTAALEALEPGEGPLLPALSEAIRRKTGVQVPLDV
                     WDVADLPPHLLMNFKVLDDEGRELAMGRDLPALRAQLGVKARRQFSERARAQFERRGL
                     TGWDFGELPEQVEFTRGGQTLIGYPALVDEGSSIALVVLDTEHDAQAATRKGLRRLFQ
                     LAAPEQVKQLARNLPGFQEMSLRYALLLEIEGGKPEKGAVSDRLRQELTDAICDRAFF
                     VEDEPIRDAAAFQARAVKARTRLMDVAGEVCRVMNEILGEYQALRPRINQPGVPVWQR
                     AMTDIRNQLRALLGPGFVVDTPLPRLKQIPRYLRAIQLRLDKFSLNPAKDAQWMQQIQ
                     GWWQAWEARVQADRKRGVHDPRLEEFRWMLEELRVSLWAQQLKTPYPVSFKRLEKYWA
                     ELG"
     gene            6165..6593
                     /locus_tag="VD791_05355"
     CDS             6165..6593
                     /locus_tag="VD791_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019452005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXV07433.1"
                     /translation="MKTVRQIIEAKTHKLLSIAPDATVYDALKLMAERDVGALVVMDG
                     ERLAGIFSERDYARKVILFGKASKDTTVREIMTDKVLCVRPEQTMDQCMALMTDKRVR
                     HLPVLDHKHVIGVISIGDVVKEVISEQQFLIEQLEQYIHM"
     gene            6666..6905
                     /locus_tag="VD791_05360"
     CDS             6666..6905
                     /locus_tag="VD791_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07434.1"
                     /translation="MLLLVCAAAAAGCATPERLTAEAIGCGTRQLKIVPSVYQQRGTE
                     TAWCAVCKDQRYHCVTNASRSRVECRESREGDGCL"
     gene            6977..>7398
                     /locus_tag="VD791_05365"
     CDS             6977..>7398
                     /locus_tag="VD791_05365"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HXV07435.1"
                     /translation="MPGHLDIFPLGDRALVIEVADSVSEAATGRARRLAERVTRARLP
                     GVREVVPAFCSITVHYDPFALCALGLPFEILSTRLLALLSGPEDETPPPGKVLEIPVC
                     YGGDCGEDLAALALARDMTPARFAELHCAPTYFVGMLG"
BASE COUNT         1130 a         2577 c         2614 g         1077 t
ORIGIN      
        1 cttctcgcgc ttcaccgacg actacgacgc ggtggtcacc ccgccggccg ccggcgaggc
       61 gcccgcctcg ctcgacacca ccggcgaccc cagcttctgc accatctgga cgctgatcgg
      121 cgtgcccgcc atcgtcattc ccaccgggct gggcagccac ggcctgccgc tggcgatgca
      181 gatcatcggc aaccagggcg agtcgaacca tctgctcgcg acctccatgt ggtgcgagcg
      241 ccagctgccg ttccgcggtc tgatcgcgcg cgagctgctc tgagccggcc tgcgcgtcgc
      301 agccgcgcaa cccctattcc acggactccg gcaccgggcc cttcacctgg aacacctcgc
      361 gcaggtaggc gacgtaggac gggtcgtagt ccatggtctt ttccggcgtg tcgctgatct
      421 tcgccaccgg ctggccgttg cagcgggtca tcttgatgac gatctgcagc ggccggtagc
      481 ccagatcgtt ggtcagattg gtccccaggc cgaaggcggg cttgcagcgg gtgtggaagc
      541 ggcggtacag ctcgacgcac agcgggatgt tcaggctgtc gctgaacacg aaggtcttgg
      601 tgcgggcgtc cacccgcatg gcgcggtagt gcgcgagcag cttctcgccc cactcgaacg
      661 ggtcgcccga gtcgtggcgc acgccgtcga acagcttgca gaagaacaga tcgaaatcgt
      721 gcaggaaggc gtccacgccg cagatgtcgg tgagcgcgat ccccagatcg ccgcggtact
      781 ccttggccca ggtgtcgaag gcgaacttct gcgagtcgcg caggcgcggc cccaccgcct
      841 ggcaggcctg cagatactcg tgcgccatgg tgccgagcgg gatcaggtcc tcgctcctgg
      901 cgagccagac gttgctggtg ccgacgaacc gttcgttcat gcgcgccttc agcgtgcgca
      961 ccaccgtctg ctgccactgg cgcgagaagc ggcggcgggt gccgaaatcc gagatcttga
     1021 agccggcgtc cggatagccg gtgatggtgc cgatcttctg ctccagcctg cgcgtgcctt
     1081 ccgcgtagtc cgggtccggc tgcgtggcgc ggaaatacgt ctcgctgacg atggccagca
     1141 ccgggacctc gaacaggatg gtgtgcagcc aggggccgcg gatggcgatg tccaggccgc
     1201 cgctgacctg cgggctctcc tcgacgtgga tgaagccctc gtcgagctgg aacaggcgca
     1261 gcagatcgac gaagtcgcct ttcatgaagc gcagcccgcg caggtaatcg agctcctcgc
     1321 gctggaagcg cagcgagcac agatgccgga tctggcggcg gatctctccg gcatgcgccg
     1381 tcagatcgac ctccggcgtg cggcactgga agcggtactc caccatggcc ccggggaaat
     1441 ggtgcagcac cacctgcatc atggtgaact tgtacagatc ggtgtcgagc agggactgga
     1501 tgatcatcgc tggaggtgct tgtgcatcgc cacggcgctg gccggacaca ggtggcggaa
     1561 ctcgctggtc tcctgcaccg cctgcggcag ggcggagcgc tccacgcgca cgtagccgcg
     1621 tgcggcgaac cagtcggctg cggtggtggt gagcaggtac agatccgtca ccttgcccgc
     1681 ccgcgccagc gcttcggcct cgtcgcacag gcgtgaaccg acccccaggc cgcgctggtc
     1741 gggggcgacc gctagcgagc gcagcagcgc ggcatgatcg agcagctgga gaccgaccac
     1801 accggcgagc ccggcggcgc cgcgcgcgcg gatgaagcgg cgcagcaggt ccgggtccac
     1861 gtcctcgacc ggcaacccgc actgcgcgag caggcgcagg acctgctggt gttcgtctgg
     1921 cgagacgcgc ccgaactgca tcccttctcc cgcgcaaccg accgatccag gcagtctgcc
     1981 cggcccgggc cgggggcgca agcccgctga cggaatcgaa cccgctggta gaatcatcga
     2041 acccggtccc gttccgggac ggtccgtcct cccgccttga acccgcagcg cgagccccag
     2101 tccaccgcgt ccgaagcccc cgacctgcgc gcgctgcagc gcctggttgc gcacaccatg
     2161 ctgcgcgacc gccaccggct gcgcagcgcg cttgcgcgcg cattcgacgc cgagcgcgcg
     2221 cagcggctgt cggcgaccga gacgctcgct ctggccgccg acatcgagcg ctctgcggcg
     2281 ctcgccaggg agcggcgcgc gcgcctgccg cgcgtgcgct acccggagga gctcccggtg
     2341 agcgagcgca agggcgagat cgcgacggcg ctcgccgcgc atcaggtggt gatcgtgtcg
     2401 ggggaaaccg gctcgggcaa gaccacgcag ctgcccaagg tctgcctgga gctcgagcgc
     2461 ggcgtggcgg ggatgatcgc ccacacgcag ccgcgccgca tcgcggcgcg caccgtggcg
     2521 gcgcgcatcg cgcaggagct gggctcgccg ctggggcagg acgtgggcta ccgggtgcgc
     2581 ttcagcgacc gcgtgagcga gcgcaatttc ctgcgcgtga tgaccgacgg catcctgctc
     2641 gccgagacgc agaccgaccg catgctggcc gcctacgaca cggtcatcat cgacgaggcg
     2701 cacgagcgca gcctcaacat cgacttcctg ctgggctacg tcaagcgcct gctgccgagg
     2761 cgcccggagc tgaagctgat cgtcacctcg gccacgatcg acgcgcagcg cttctccgcg
     2821 cacttcgacg gcgcgccggt gatcgaggtg tccggccggc tctatccggt cgacgtgcgc
     2881 tatcgcccgc tggcggcgca gggcgaggac gagcaggaga tcgatctgcc cgaggccatc
     2941 gccgacgcgg tggaccaggt cgcccggctg tccgccggcg gagacgtgct ggtgttcctg
     3001 cccggcgagc gcgagatccg cgagaccgcg gaggtgctgc gcaagcacgt gttccgcggt
     3061 gcggcgcgcg cgcagcacgt cgagatcctg ccgctctacg cgcgcctgtc cgcgcaggag
     3121 caggagcgcg tgttccagcc cgccggcatg cgccgcatcg tgctcgccac caacgtcgcc
     3181 gagacgtcgc tgaccgtgcc cggcatcaag tacgtggtcg actcgggcct ggcgcgcgtc
     3241 aatcgctaca gctaccgcaa caagcacgag atgctgcagg tggagaagat ctcgcgcgcc
     3301 tccgccgacc agcgcgccgg ccgctgcggc cgcgtgatga gcggcgtctg cgtgcgcctg
     3361 tacgccgagg aggattatca ggcgcggccg gccttcaccg atccggagat cctgcgctcc
     3421 tcgctcgcca gtgtgatcct gcgcatgaag gcgctgaagc tgggcgaggt ggaggacttc
     3481 cccttcgtcg agccgccgtc cgcccgcgcc atcgccgacg gctacagcct gctcgtcgag
     3541 ctgggcgcgg tggacgccga gcggcgcctg acgccggtgg gggcgcggct cgccgagctg
     3601 cccatcgatc ccggcctggc gcgcatgatc gtggcggcca gggacgagaa ctgcctcacc
     3661 gagatgctgg tggtgagcgc cggcctggcg atccaggacg tgcgcgagcg gccgctggac
     3721 aaggccgagg ccgccgacac cgcgcacgcg ccgttcgccg acgagcgctc cgagttcctc
     3781 ggcctgctca agctgtgggc tttcttcgag gacgcgctca ggcacaagaa gtcgaaccgc
     3841 aggctcgcgc agctgtgccg cgacagcttc ctgtcctacg tgcggctgcg cgagtggcgc
     3901 gatctgcacg gccagctgca tgcgcaggtg tcggagctgg gctggcgctt caaccaggcg
     3961 cccgccaccc acgagcagct gcaccgggcg atcctcaccg gcttgctggg caacgtcggc
     4021 gccaagtccg aggaggcggg cgtgtacctg ggcgcgcgcg ggatcaagtt cgcgatcttt
     4081 cccggctcgc cgctgcgcaa gaagggcccg ccctggctga tggccgcgga gctcatcgag
     4141 accacgcgcc tgtacgcgcg caccgtggcc gcgatcgaca cgcagtggct ggagcgcgtc
     4201 ggcgcgcacc tcaccaagac gacctggcac gacccgcact gggagaagag cggagggcag
     4261 gcctacgcgt acgagcgcgt cacgctctac ggcctggtgg tggtgtcgcg ccggcgcgtg
     4321 gcgttcggtc cggtggaccc gaaggcggcg cgagagatct tcatccgcga ggcgctggtg
     4381 cacggccagc tcgccaccca cggcggattt ctgcggcaca accgggccct gcgggaggag
     4441 atcgaggcgc tcgagcacaa gtcccgccgc cacgacgtgc tggtggacga cgaagcggtg
     4501 ttcgccatct tcgacgccat cctgccgcag gacgtgtgga gcgcgcagca gttcgagcgc
     4561 tggcggcgtg aggcggagcg cgccagcccc aggctgctgt ttctcacccg cgagcagctc
     4621 atgcgccacg ccgcgcagga gatcaccgag cagcgcttcc ccgcggcgat cgagatcggc
     4681 ggcgtggcgt acgacttgtc ctaccggttc gagcccggcc atccgctcga cggcgtcacc
     4741 ctgagcgtgc cgctgcacct gctgaaccag ctcgacgagc gccgctgcga atggctggtg
     4801 ccgggcctgc tgcgcgacaa ggtgagccac ctgctgcgcg gtctgcccaa gaacctgcgg
     4861 cgcaatttcg tcccggcgcc gcaatacgtc accgccgcgc tcgaggcgct ggagcccggc
     4921 gaaggcccac tgctgccggc cctttcggag gcgatccggc gcaagaccgg cgtccaggtc
     4981 ccgctggacg tctgggacgt ggccgacctg ccgccccacc tgctgatgaa cttcaaggtg
     5041 ctggacgacg agggccgcga gctcgccatg ggccgcgacc tgcccgcgct gcgcgcgcag
     5101 ctcggcgtga aggcgcgacg ccagttcagc gagcgcgcgc gcgcgcagtt cgagcgcaga
     5161 gggctcaccg gctgggattt cggcgagctg cccgagcagg tggagttcac ccgcggcggg
     5221 cagacgctga tcggctaccc ggcgctggtc gacgagggca gctcgatcgc gctggtggtg
     5281 ctggataccg agcacgacgc gcaggccgcc acccgcaagg gcctgcgcag gctgttccag
     5341 ctggccgcgc ccgagcaggt caagcagctc gcgcgcaacc tgcccggctt ccaggagatg
     5401 agcctgcgct atgcgctgct gctcgagatc gaaggcggca agccggagaa aggcgcggtg
     5461 tccgaccggc tgcgccagga actgaccgac gccatctgcg atcgcgcgtt cttcgtggag
     5521 gacgagccga tccgcgacgc cgccgcgttc caggcgcgcg cggtcaaggc caggacccgg
     5581 ctgatggacg tggccggcga ggtgtgccgg gtgatgaacg agatcctcgg cgaataccag
     5641 gcgctgcgcc cgcgcatcaa tcagccggga gtgccggtgt ggcagcgggc gatgaccgat
     5701 atccgcaacc agctgcgcgc gctgctcggg cccggtttcg tggtcgacac gccgctgccg
     5761 cggctcaagc agatcccgcg ctacctgcgg gcgatccagc tccggctcga caagttcagc
     5821 ctcaatccgg ccaaggacgc gcagtggatg cagcagatcc agggctggtg gcaggcctgg
     5881 gaggcgcggg tgcaggccga ccgcaagcgc ggcgtgcacg atccccggct cgaggagttc
     5941 cgctggatgc tggaggagtt gcgcgtgtcg ctgtgggcac agcagctcaa gacgccctac
     6001 ccggtctcgt tcaagcgcct ggagaagtac tgggcggagc tcggctgacc ggcggttcac
     6061 aggagttgca gcgggaatcg gcccggggct aatatggtcc cggccggcgg ccgccggggc
     6121 acacgcgcgc gccggcgccg acctggaccc agggggaaga ggccatgaag accgtccggc
     6181 agatcatcga agccaagacg cacaagctgc tgtccatcgc gcccgatgcg accgtgtacg
     6241 acgcgctcaa gctgatggcc gagcgcgacg tcggagcgct ggtggtgatg gatggcgagc
     6301 ggctcgcggg gatcttctcc gagcgcgact atgcgcgcaa agtgatcctg ttcggcaagg
     6361 cctcgaagga caccaccgtg cgcgagatca tgacggacaa ggtgctgtgc gtgcgcccgg
     6421 agcagaccat ggaccagtgc atggcgctga tgaccgacaa gcgcgtgcgt cacctgccgg
     6481 tgctggacca caagcacgtg atcggcgtga tctccatcgg cgacgtggtc aaggaagtca
     6541 tctccgagca gcagttcctg atcgagcagc tggagcagta cattcacatg taagccgccc
     6601 gcaggcggcg gcccgatgcc gcgtgccggc ccggtcgcgc cgatgcaaca ccgatgtcct
     6661 tgcgagtgct tctcctggtt tgcgcagccg ctgcagcagg gtgcgccact cccgaacggc
     6721 tgaccgccga ggcgatcggc tgcggcaccc ggcagttgaa aatcgtcccc agcgtgtatc
     6781 agcagcgcgg caccgagacc gcctggtgcg ccgtgtgcaa ggaccagcgc tatcactgcg
     6841 tgaccaacgc cagccgctcg cgtgtggagt gccgcgagag ccgggagggc gacggctgcc
     6901 tgtagccgcc cagacccggt tcgatgcccg ccgcgcgctc gcccgcgaga tgcgcgggcc
     6961 ggcacagtca ccccgcatgc ctggccacct cgacatcttt cccctcggcg accgcgccct
     7021 cgtgatcgag gtcgcggaca gcgtgagcga ggccgccacc ggccgcgcgc ggcgcctcgc
     7081 ggagcgcgtg acgcgcgcac gtctgcccgg cgtgcgcgag gtggtgccgg cgttctgctc
     7141 gatcaccgtg cattacgacc cgtttgcgtt gtgcgcgctc ggtctgccct tcgagatcct
     7201 gagcacgcgc ctgctggcgc tgctgtcggg tccggaagac gaaacgccgc cgcccggcaa
     7261 ggtgctggag atcccggtct gctacggcgg cgattgcggc gaggacctgg ccgcgctggc
     7321 cctggcccgc gacatgacgc ccgcccgttt cgcggaactg cactgcgcgc cgacctactt
     7381 cgtcgggatg ctgggctt
//
LOCUS       DASPGE010000170         7881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_514930, whole genome shotgun sequence.
ACCESSION   DASPGE010000170 DASPGE010000000
VERSION     DASPGE010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7881
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514930"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VD791_05370"
     CDS             complement(<1..765)
                     /locus_tag="VD791_05370"
                     /inference="COORDINATES: protein motif:HMM:NF017346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HXV07436.1"
                     /translation="MMSFTIHGIGVSGGIAIGRAHLLTHTQLEVEHYDIAPGDVEQEK
                     ARFDRAINSVRAELAALAEHIPANSPAEFEGFLNLHLMILNDATLSEAPKRLIEEQRA
                     NAEWALTQQAESLLTQFDRIEDEYLRERKTDVVQVVERVLKALMGQPRAIPAPLHPEE
                     DSVLVAHDLSPADVILFRQHRFASFVTDLGGATSHTAILAHSLGIPSVVALHHARQLI
                     HEGELLIVDGTHGVVIVNPDANVLSEYHLRQEQWILE"
     gene            complement(859..1128)
                     /locus_tag="VD791_05375"
     CDS             complement(859..1128)
                     /locus_tag="VD791_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HXV07437.1"
                     /translation="MQQREVEIVNKLGLHARAAAKLTQLAAGFQCEIWLSRSGRRVNA
                     KSIMGVMMLAAAKASKVAIETSGADEEQAMQAIVQLIAEKFGESE"
     gene            complement(1109..1525)
                     /locus_tag="VD791_05380"
     CDS             complement(1109..1525)
                     /locus_tag="VD791_05380"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HXV07438.1"
                     /translation="MVGILIIAHGTLGESLIHCASHVLGSRPRSLMQIGVTVHDDPLQ
                     LLPQALKLVKQLDQGHGVLVLTDVYGATPANIAAQLLVPGRVEGVAGVSLPMLVRALN
                     YRNEPLDTLARKAVSGGIEGVMRMPSAGHGNAATGS"
     gene            complement(1562..2506)
                     /gene="gshB"
                     /locus_tag="VD791_05385"
     CDS             complement(1562..2506)
                     /gene="gshB"
                     /locus_tag="VD791_05385"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HXV07439.1"
                     /translation="MKLAFVLDPLDSIKTYKDSSFAMMEEAARRGHALFVLHQEHIVC
                     RREQVTARAAAIELTGDQHDWYRLGEEKDTPLPAFDAVLMRKDPPFDMEYIYSTYLLE
                     LAERQGARIVNRPRALRDYNEKFSITGFARFTVPTLVTRLESELRAFLEEQGDIVLKP
                     LDGMGGASVFRLHRQDPNVGVVIETLTHYGRRTVMAQRYIPEIAQGDKRILVIGGKPA
                     PYALARIPKPGETRGNLAAGARGVAQPLSERDREIGETVGAALLGDGLLLVGLDVIGD
                     WLTEINVTSPTGMREIHEQTGFSVAGMAIDAIEALVRT"
     gene            complement(2503..3813)
                     /gene="gshA"
                     /locus_tag="VD791_05390"
     CDS             complement(2503..3813)
                     /gene="gshA"
                     /locus_tag="VD791_05390"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HXV07440.1"
                     /translation="MKAAELNPGLRGPLLELEQKILHNHEAIEHWMRAQWQEHETPFY
                     ASVDLRNAGFKLAPVDTNLFPGGFNNLKSEFRPLCVQAAMTAIQKLCPDAKGVLLIPE
                     SHTRNLFYLENLAQLQSILRMAGMKVRVGTLIPEIAAPTALDLPSGAKVTLEPLVRKG
                     NRIGMESFDPCVVLLNNDLSGGIPPILEGIEQQVIPPLYAGWSTRRKSRHFAAYSGVA
                     EEFGRLIDIDPWLIDPYFATCGEINFQERKGEECLAGHVEGILARVRSKYREHGIERE
                     PFCIVKADAGTYGMGVMTVKDPAEVRDLNRKQRTRMAVVKEGLPVTEVMVQEGVYSFE
                     TVGDAVAEPVIYMIDQFVVGGFYRVHTDRGQDENLNAPGMRFEPMPFDGTCLLPELES
                     NAECAPNRFYAYGVIARLALVSAAIELEQMAQGLEAEARLRKMA"
     gene            3959..5635
                     /locus_tag="VD791_05395"
     CDS             3959..5635
                     /locus_tag="VD791_05395"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HXV07441.1"
                     /translation="MPTDIEIAQQTSLRRIADLARERLAIPDDALEPYGHYKAKISLG
                     YLDSLAARPDGKLVLVTAISPTPAGEGKTTTTVGLGDALNRIGKKAVICLREPSLGPV
                     FGMKGGAAGGGYAQVVPMEDINLHFTGDFGAIGLANNLLAAMIDNHIHHGNELGIDVR
                     RITWRRVVDMNDRALRDIAVALGGTANGYPREDGFDIVVASEVMAIFCLATSLQDLKA
                     RLANIVVGYTRDQQPVRARDLKADGAMAVLLKDALKPNLVQTLEHNLAIIHGGPFGNI
                     AHGCNSVIATRAGLKLADYVVTEAGFGADLGAEKFVDIKCRKSGLRPDAVVIVATVRA
                     LKYHGGADLKALERPNPGALEKGIANLERHVNNVRNHFGLPCVVCINHFTHDTDAEIA
                     LLKRKMAHHEAPIVVARHWAEGGKGAEELAHAVADLIARVPSDFRFVYHDDQPLWEKM
                     EAVAGKIYGAAQVTADSKVRAQIARLEADGYGRYPVCVAKTQYSFSTDPALRGAPAGH
                     TVNVREVRLCAGAEFVVMICGDIMTMPGLPKVPSAENIDLAGDGRVVGLF"
     gene            5656..6123
                     /gene="queF"
                     /locus_tag="VD791_05400"
     CDS             5656..6123
                     /gene="queF"
                     /locus_tag="VD791_05400"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HXV07442.1"
                     /translation="MPRKPAAPKPIQLGRPTALPAGPEQALLERVPNPHRDATYLVRF
                     TAPEFTALCPITGQPDFAHLVIDYVPARWLVESKSLKLYLGSFRNHGAFHEDCTLAVG
                     KRLVKLLAPQWLRIGGYWYPRGGMPIDVFWQTGAPPRGLWAPDQGVAPYRGRG"
     gene            complement(6166..7353)
                     /locus_tag="VD791_05405"
     CDS             complement(6166..7353)
                     /locus_tag="VD791_05405"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HXV07443.1"
                     /translation="MSRLILVINAGSSSLKFQAFALDSAALKMRLRGQIEGIGTRPSF
                     TARDAQGTNLADQALEASQGRDQGACLAFLASWLQHAMQDAQLVGVGHRVVHGGTRYS
                     APVRINRNVLAELEALVTLAPLHQPFNLAPIRALLESRPGLGQVACFDTGFHRTQPML
                     AELFALPRRYYDDGVRRYGFHGLSYEYIAQQLPNVAPEIALQRVVAAHLGNGVSLCAM
                     QEGRSVATTMGFTALDGLPMGTRCGELDPGVVIYLARERGMSVDQIESLLYRQSGLLG
                     LSGLSHDMRELLASDSPDARLAVDYFVYRIARGVGGLAGALGGLDALVFTAGIGENSP
                     EIRARVCWRLGWLGLDFDPEANVAGAARITRPGSRVSAWVVPTNEELMIAQHTRDVLG
                     LGM"
     gene            complement(7476..>7881)
                     /locus_tag="VD791_05410"
     CDS             complement(7476..>7881)
                     /locus_tag="VD791_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006713299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HXV07444.1"
                     /translation="SMGALGVGAGASIFAYLAVAVCKEKFGYDDSLDVFGVHCIGGII
                     GALGTGIFAAPLLGGAGLYDYIQDAANPQYSIGSQFMVQLTGVITTVVWSGVVAFVAY
                     KIVDMTIGLRVTEEQEREGLDETQHGERAYHM"
BASE COUNT         1226 a         2718 c         2679 g         1258 t
ORIGIN      
        1 ctccaggatc cactgttcct ggcgcaggtg gtactccgac agcacgttgg cgtcggggtt
       61 gacgatcacc acgccgtggg tgccgtccac gatcaacagc tcgccctcgt ggatcagctg
      121 gcgcgcgtgg tgcagggcga ccaccgaggg gatgcccagg ctgtgcgcca ggatcgcggt
      181 gtgcgaggtg gccccgccca ggtcggtgac gaagctcgcg aagcgatgct gcctgaacag
      241 gatcacgtcg gcgggcgaca ggtcgtgcgc caccaggacg ctgtcctcct ccgggtgcag
      301 cggcgccgga atggcgcgcg gctggcccat gagcgccttg agcacgcgct cgaccacctg
      361 caccacgtcg gtcttgcgct cgcgcagata ctcgtcctcg atgcgatcga actgggtgag
      421 cagcgactct gcctgctgcg tcagcgccca ttcggcgttg gcgcgctgct cctcgatcag
      481 ccgcttgggc gcctccgaca gggtggcgtc gttgaggatc atcaggtgca ggttgaggaa
      541 accctcgaac tccgcgggcg agttcgcggg gatgtgctcg gccagggcgg ccagctcggc
      601 gcgcacgctg ttgatcgccc ggtcgaagcg cgccttctcc tgctcgacat cgccgggggc
      661 gatgtcgtag tgctcgacct cgagctgcgt gtgcgtcagc aggtgggcgc ggccgatggc
      721 gatgccgccg gagacgccga tgccgtggat ggtgaaggac atcatgtaga cgtgattcgt
      781 gattcgtgat tcgtgatggg tgattcctaa tcgacccccg tgatcctacg aatcaccaat
      841 cacgcttcac gaatcacatc actcgctttc cccgaatttc tccgcgatca gctgcacgat
      901 cgcctgcatc gcctgttcct cgtcggcgcc gctggtctcg atggcgacct tcgaggcctt
      961 ggccgcggcc agcatcatca cacccatgat gctcttcgcg ttcacccggc ggccgctgcg
     1021 cgacagccag atctcgcatt ggaagccggc ggccagctgg gtcagcttgg ccgctgcgcg
     1081 ggcgtgcagc ccgagcttgt tgacgatctc aacttcccgt tgctgcattg ccatgacccg
     1141 cggacggcat gcgcatcacc ccctcgatgc cgccggatac cgccttgcgg gcgagcgtgt
     1201 cgagcggctc gttgcggtag ttgagcgcgc gcaccagcat cggcaggctc actcccgcaa
     1261 ccccttccac gcggcccggg accagcagct gggcggcgat gttggccggc gtggcgccgt
     1321 acacgtcggt gagcaccagc acgccgtggc cctggtcgag ctgcttgacc agcttgagcg
     1381 cctgcggcag cagctgcagg gggtcgtcgt gcacggtcac gccgatctgc atcagggacc
     1441 gcggacggct gcccagcacg tggctggcgc agtggatcag gctttcgccg agggtgccgt
     1501 gggcgatgat gaggattccg accatcgagg ttcaaggccg tgtcgacggc cgcattctac
     1561 gtcaggtgcg caccagcgcc tcgatggcgt cgatcgccat cccggccacg ctgaacccgg
     1621 tctgctcgtg gatctcccgc atgccggtgg ggctggtcac gttgatctcg gtcaaccagt
     1681 cgccgatcac gtccagcccg accaggagca gaccgtcgcc cagcaatgcg gcgcccaccg
     1741 tctcgccgat ctcgcgatcg cgctcggaca gcggctgcgc gacgccgcgc gcgccggcgg
     1801 cgaggttgcc gcgggtctca ccgggcttgg ggatgcgcgc cagcgcgtaa ggcgcgggct
     1861 tgccgccgat caccaggatg cgcttgtcgc cctgggcgat ctcggggatg tagcgctgcg
     1921 ccatcacggt gcggcggccg tagtgcgtga gcgtctcgat caccacgccg acgttggggt
     1981 cctgccggtg caggcggaac accgaggccc cgcccatgcc gtccaggggc ttgagcacga
     2041 tgtcgccctg ctcttcgaga aaggcccgca gctcgctctc cagccgggtg accagggtgg
     2101 gcacggtgaa gcgggcgaag ccggtgatcg agaacttctc gttgtagtcg cgcagcgcgc
     2161 gggggcggtt gacgatgcgc gcgccttgcc tctcggccag ctccagcagg taggtgctgt
     2221 agatgtactc catgtcgaag ggcgggtcct tacgcatcag caccgcgtcg aacgccggca
     2281 acggcgtgtc cttctcctcg cccagccggt accagtcgtg ctggtcgccg gtcagctcga
     2341 tcgccgccgc gcgcgccgtg acctgctcgc ggcggcagac gatgtgctcc tggtgcagca
     2401 cgaacagggc gtgtccgcgc cgcgcggcct cctccatcat ggcgaaggag gagtccttgt
     2461 aggtcttgat cgaatcgagg gggtcgagga cgaaggcgag cttcatgcca tcttgcgcag
     2521 ccgcgcctcg gcctccaggc cctgcgccat ctgctccagc tcgatggcgg cggacaccag
     2581 cgccagccgg gcgatcacgc cgtaggcata gaagcggttg ggtgcgcact cggcgttcga
     2641 ttccagctcg ggcagcagac aggtgccgtc gaagggcatc ggctcgaagc gcatgcccgg
     2701 cgcgttcagg ttctcgtcct ggccgcggtc ggtgtgcacg cggtagaagc cgcctaccac
     2761 gaactggtcg atcatgtaga tcaccggctc ggccaccgcg tcgccgacgg tctcgaagct
     2821 gtacacgccc tcctgcacca tgacctcggt gaccgggagc ccctccttga ccaccgccat
     2881 cctggtgcgc tgcttgcggt tgagatcgcg cacctcggcg gggtccttca ccgtcatcac
     2941 gcccataccg taggtgccgg cgtcggcctt gacgatgcag aagggctcgc gctcgatacc
     3001 gtgctcgcgg tacttgctgc gcacccgcgc caggatgccc tcgacgtggc cggcgaggca
     3061 ttcctcgccc ttgcgctcct ggaagttgat ctcgccgcag gtggcgaagt aggggtcgat
     3121 cagccacggg tcgatgtcga tcagccgccc gaattcctcc gccacgccgc tgtaggcggc
     3181 gaaatggcgc gacttgcgcc gcgtggacca gccggcgtag agcggcggga tcacctgctg
     3241 ctcgatgccc tccaggatcg gcgggatgcc gccggacaga tcgttgttga gcagcaccac
     3301 gcaggggtcg aagctctcca tgccgatgcg gttgcctttg cgcaccagcg gctcgagcgt
     3361 gaccttggcg ccgctcggaa gatcgagcgc agtcggtgcg gcgatctcgg gaatcaacgt
     3421 ccccacccgc accttcatcc ccgccatgcg caggatcgac tgcagctgcg ccaggttctc
     3481 gaggtagaac aggttgcgcg tatggctctc cgggatcagc agcacgccct tggcgtcggg
     3541 gcacagcttc tggatcgcgg tcatcgcggc ctgcacgcac agcggccgga actcggactt
     3601 caggttgttg aagccgccgg ggaacagatt ggtgtccacc ggcgccagct tgaagccggc
     3661 gttgcgcaga tccaccgacg cgtagaacgg cgtctcgtgc tcctgccact gcgcccgcat
     3721 ccagtgctcg atggcctcgt ggttgtgcag gatcttctgc tcgagctcga gcagcggtcc
     3781 gcgcagcccg gggttcagtt cagccgcttt catggggact cgccagggtg gggaggccat
     3841 tgtagccaaa gcggctgtcc ccgacagtgg ccggccccgg ggttcagacc ccggcgtaag
     3901 accccatgat aagaccgagg cgtagaatgg cgccctttcc cgccgtctcc cggttcgcat
     3961 gcccaccgac atcgagatcg cccagcagac cagcctgcgc cgcatcgccg acctcgcgcg
     4021 cgagcgcctg gccatccccg acgacgcgct ggagccctac gggcactaca aggccaagat
     4081 ctcgctcggc tacctcgact cgctggcggc gcggcccgac ggcaagctgg tgctggtcac
     4141 cgccatcagt ccgacgccgg cgggcgaagg caagaccacc acgacggtgg ggctgggcga
     4201 cgcgctcaac cgcatcggca agaaggcggt catctgcctg cgcgagccgt cgctcgggcc
     4261 ggtgttcggg atgaaaggcg gggcggccgg cggcggttac gcgcaggtgg tgccgatgga
     4321 ggacatcaac ctgcatttca ccggcgactt cggcgccatc gggctggcga acaacctgct
     4381 cgccgcgatg atcgacaacc acatccacca cggcaacgag ctcggcatcg acgtgcgccg
     4441 catcacctgg cggcgcgtgg tggacatgaa cgaccgcgcg ctgcgcgaca tcgccgtggc
     4501 gctgggcggc acggcgaacg gctatccgcg cgaggacggc ttcgacatcg tggtcgcctc
     4561 cgaggtgatg gcgatcttct gcctggcgac ctcgctgcag gatttgaagg cgcggctcgc
     4621 caacatcgtg gtcggctaca cccgcgacca gcagccggtg cgcgcgcgcg acctcaaggc
     4681 cgacggcgcc atggcggtgc tgctgaagga cgcgctcaag cccaacctgg tgcagacgct
     4741 ggagcacaac ctggcgatca tccacggcgg tccgttcggc aacatcgcgc acggctgcaa
     4801 ctcggtgatc gccacccgcg ccgggctcaa gctcgccgat tacgtggtga ccgaggccgg
     4861 cttcggcgcc gatctgggcg cggagaagtt cgtcgacatc aagtgccgca agtccggcct
     4921 gcgccccgac gcggtggtga tcgtcgccac cgtgcgcgcg ctcaagtatc acggcggcgc
     4981 cgacctcaag gcgctggagc ggccgaatcc gggggcgctg gagaagggca tcgccaacct
     5041 cgagcggcac gtgaacaacg tgcgcaacca cttcggtctg ccctgcgtgg tgtgcatcaa
     5101 tcacttcaca cacgacaccg acgccgagat cgccctgctg aagcgcaaga tggcgcatca
     5161 cgaagcgccg atcgtggtcg cgcgccactg ggccgagggc ggcaagggtg ccgaggagct
     5221 ggcgcacgcg gttgcggacc tgatcgcgcg ggtgccgtcg gacttccgct tcgtctacca
     5281 cgacgaccag ccgctgtggg agaagatgga ggcggtggcc ggcaagatct acggcgcggc
     5341 ccaggtcacc gccgacagca aggtgcgcgc gcagatcgcc aggctggagg ccgacggcta
     5401 cggccgttac ccggtgtgcg tcgcgaagac gcagtactcg ttctccaccg acccggcgct
     5461 gcgcggcgcg ccggccggac acacggtgaa cgtgcgcgaa gtgcgcctgt gcgccggcgc
     5521 cgagttcgtg gtgatgatct gcggcgacat catgaccatg cccggcctgc cgaaggtgcc
     5581 ttcggccgag aacatcgatc tcgccgggga tggcagggtg gtgggcctgt tctgacggcg
     5641 gctggcgaga cggccgtgcc acgcaagccg gcagcaccga agccgatcca gctcgggcgt
     5701 cccactgcgc tgcccgccgg tccggagcag gcgctgctcg agcgcgttcc caatccgcat
     5761 cgcgacgcga cgtacctggt gcgcttcacc gcgcccgagt tcaccgccct gtgtccgatc
     5821 accggccagc ccgatttcgc gcatctggtg atcgactacg tgccggcgcg ctggctggtg
     5881 gagagcaagt cgctcaagct ctacctcggc agcttccgca accacggcgc cttccacgag
     5941 gactgcacgc tcgccgtggg caagcgcctg gtgaagctgc tcgcgccgca gtggctgcgc
     6001 atcggcggct actggtaccc gcgcgggggc atgccgatcg acgtgttctg gcagaccggc
     6061 gcgccgccgc gcggcttgtg ggctcccgac cagggggtcg ccccttaccg cgggcgaggc
     6121 tgactcaggg aagctcgcgc agcgcgatac tcgttcaggg cttccctaca tccccaggcc
     6181 gagcacgtcg cgcgtgtgct gcgcgatcat cagctcctcg ttggtgggca ccacccaggc
     6241 cgacacgcgg ctgccggggc gcgtgatgcg cgctgcgccg gcgacgttgg cctccgggtc
     6301 gaaatccagg cccaaccatc ccagccgcca gcacacgcgc gcccggatct ccgggctgtt
     6361 ctcgccgatg ccggcggtga acaccagcgc gtccagcccg cccagcgcac cggccagccc
     6421 gcccacgccg cgcgcgatgc ggtacacgaa atagtccacc gcaagccgcg cgtccgggct
     6481 gtcggaggcg agcagctcgc gcatgtcgtg gctcagcccg gacaggccca gcaggccaga
     6541 ctgccggtac agcagcgact cgatctggtc gacgctcatg ccacgctcgc gcgcgagata
     6601 gatcaccacg ccgggatcga gctcgccgca gcgcgtgccc atcggcagcc catccagggc
     6661 ggtgaagccc atggtggtgg ccacgctgcg cccctcctgc atggcgcaca ggctcacgcc
     6721 gttgcccagg tgcgcggcca ccacgcgctg cagcgcgatc tccggcgcca cgttcggcag
     6781 ctgctgcgcg atgtactcgt aggacagccc gtggaaaccg tagcggcgca cgccgtcgtc
     6841 gtagtagcgg cgcggcagcg cgaacagctc ggccagcatc ggctgcgtgc ggtggaagcc
     6901 ggtgtcgaag caggcaactt gtcccagccc ggggcgcgac tccagcagcg cacggatcgg
     6961 cgccaggttg aacggctggt gcaggggcgc gagcgtcacc agcgcctcca gctctgccag
     7021 cacgttgcgg ttgatgcgca ccggggccga gtaacgggtg ccgccgtgaa ccacgcgatg
     7081 gcccacgccc acgagctgcg catcctgcat ggcgtgttgc agccacgatg ccaggaaggc
     7141 cagacacgcc ccttggtcgc gtccctggct cgcctccagc gcctggtccg cgaggttcgt
     7201 tccctgcgca tcgcgcgcgg tgaagctcgg acgcgttccg atgccctcga tctgcccgcg
     7261 caggcgcatc ttcagggcgg cgctgtcgag cgcgaacgcc tggaacttca agctcgagga
     7321 tccggcgttg atgaccagga tcaggcggct catggggcac gattatcgcg cggtttcgcg
     7381 ccccgggtcg ctggctgcgc gagaaaaaaa gcgccccgca gggcgctttt gcaaccggcg
     7441 atcgagacgc cgggggagga ggctgcagca aaaagttaca tgtgatacgc gcgctcgccg
     7501 tgctgggtct cgtccaggcc ttcgcgctcc tgctcttcgg tcacgcgcaa gccgatcgtc
     7561 atgtccacga tcttgtaggc gacgaaggcc accacgcccg accacactac cgtggtgatc
     7621 accccggtga gctggaccat gaactggctg ccgatcgagt actgggggtt tgcggcgtcc
     7681 tggatgtagt cgtacagacc ggcgccaccc agcaaaggcg cggcgaagat cccggtaccg
     7741 agcgccccga tgatgccgcc gatgcagtgc acgccgaaca cgtccagcga gtcgtcgtag
     7801 ccgaacttct ccttgcagac cgcaacggcc aggtaggcga agatgctggc gccggcgccc
     7861 acgcccagcg cacccatgga a
//
LOCUS       DASPGE010000171         5776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_982711, whole genome shotgun sequence.
ACCESSION   DASPGE010000171 DASPGE010000000
VERSION     DASPGE010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982711"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="VD791_05415"
     CDS             <1..1418
                     /locus_tag="VD791_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV07445.1"
                     /translation="GAIVIQRREPGAFAVSHVELLKTFADQAVIAIENVRLFKELEAR
                     TSDLGRSVEQLKALSEVGQAVNSTLDLQEVLNTIVARAVQLSAADTGAVFEHDEASAM
                     LELRATYGINPELVDELMVRTLRMGEGATGRAAATRAPVEIADVQAVDVYTGKMRDVL
                     DRAGYRAMLAVPLVQGERVLGGLTVTRRTPGKFPPEVVDLLQTFATQSTLAIQNARLF
                     REIEQKGRELEIASQHKSQFLANMSHELRTPLNAILGYTELVADGIYGAVPQKIREVV
                     ERVQKSGRHLLGLINDVLDISKIEAGQLKLSVGDYSFHDVVQSVTTAVESLAAEKKLA
                     LKVDLPGDLPVAAGDERRITQVLLNLVGNAIKFTDTGEVGITVRAADSKFLVAVRDTG
                     PGIDAEHQQRIFEEFQQVDSSPTKTKGGTGLGLAIAKRIVEMHGGHIWVESTLGLGST
                     FFVQLPLRTAPQDIAASTSAS"
     gene            1519..1884
                     /locus_tag="VD791_05420"
     CDS             1519..1884
                     /locus_tag="VD791_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV07446.1"
                     /translation="MSKRILVVEDQEDNRRIMRDLLSASGYAYIEATTGDEGLAAAQR
                     EKPDLILMDIQLPGMDGYEATRRIKADPALKHVPIIAVTSYALSGDDDKAFAAGCDGY
                     VTKPFSPKVLLAKIREYLK"
     gene            1904..3019
                     /locus_tag="VD791_05425"
     CDS             1904..3019
                     /locus_tag="VD791_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV07447.1"
                     /translation="MHDPPRILVVDDNAANVEILQMRLLANGYEVVTASDGVEALSAT
                     RRHLPDLILLDVMMPKMDGIEVCRRLRADASLPFIPIVLVTAKSDPKDVVAALEAGGD
                     EYLTKPVDQTSLVARVKSMLRIKGLHDTVQQQSAQLKEWNAGLEQRVAQQVGELERFG
                     RLKRFFSPQLVESILAGGAEDPLKSHRREVVVVFLDLRGFTAFSETAEPEEVMGVLRE
                     YHQHMGGMVLAHEGTLERFAGDGMMVFFNDPVQVPDPAARAVRMAASMRERLGELTAR
                     WRKRGFALDFGAGIAQGYATIGAIGFEGRWDYGAIGTVTNLASRLCDEASPGQVLLSQ
                     RVLSGVEDLVEVEPVGALQLRGFLKPVQTYNLIGMKA"
     gene            complement(3093..4574)
                     /locus_tag="VD791_05430"
     CDS             complement(3093..4574)
                     /locus_tag="VD791_05430"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXV07448.1"
                     /translation="MQSVENLSRDELVARLRALQAGADPDFVSRQALGALDNPALIDL
                     VNESRQRLALALESAALALWEWDIPGGRVFYSDELAGMLQVPPGTLPQDTYPCQHLAH
                     PEDSASLAEGLNAHLRVPSEPFERSLRVRLGSGRWKLLLARGRVIGRDRDGNALRMAG
                     TVQDLDACHLMPSPTGEARPAAAQDDAAGAATAANAWREHQDQLESILNNAAEGMLVV
                     SAEGGIERINPVAQRMFGYGADQALRTNLRHLTVELDYDGEPAAAEESPAQRMRRLLG
                     GRREVTGRRQDGSLFPLELSLSEITLMPGPPKFAALVRDITERKTWENRIYSLAYSDS
                     LTGLPNRLLLRDRLEQAIASAQRNRSQVGVLFVDLDHFKAVNDSYGHHAGDQLLREIG
                     ERAKSCVREIDTVCRLGGDEFVLVLPDLREVGDAGAVARKLVAALSAPYAIEGRELAI
                     TPTVGVSIYPHHGADADTLIRNADTAMYHAKESGKNKYRFYAP"
     gene            4778..5515
                     /locus_tag="VD791_05435"
     CDS             4778..5515
                     /locus_tag="VD791_05435"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXV07449.1"
                     /translation="MFLPIADEPNPRHFPAMTVALIAINVAVYLLVSLPLGMRPPDPT
                     DPALAEYLRALLPHLPPGTSAREVLQQMSAYDLVVYGWGFRPAAPSLVTLVTAMFLHG
                     GLMHLFGNMLYLWIYGNNVEHRLGMPAYLFWYLATGIAATLFHTLFNVGSPIPLVGAS
                     GAISGVLGFYLIWFPHHTVKVWVFLFPIYMGMVHISATIVLVVYLLIDNLLPFLAAPA
                     GGIAHGAHIGGFIAGAMVALILRRKRR"
     gene            complement(5654..>5776)
                     /gene="mscL"
                     /locus_tag="VD791_05440"
     CDS             complement(5654..>5776)
                     /gene="mscL"
                     /locus_tag="VD791_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796739.1"
                     /note="homopentamer; channel that opens in response to
                     pressure or hypoosmotic shock; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HXV07450.1"
                     /translation="IFLMIKQFNRLKRQAPPPAPAAPPEEVVLLRDIRDSLRRA"
BASE COUNT          935 a         1900 c         2007 g          934 t
ORIGIN      
        1 tcggcgccat cgtcatccag cggcgcgagc cgggggcctt cgcagtcagc cacgtcgagc
       61 tgctcaagac cttcgcggac caggccgtca tcgccatcga gaacgtccgc ctgttcaagg
      121 aactcgaggc gcgcacctcc gatctcggac gctcggtgga gcaactcaag gcgctgtccg
      181 aggtgggcca ggcggtgaac tcgacgctgg atctgcagga ggtgctgaac acgatcgtgg
      241 cgcgggcggt ccagctcagc gcggccgaca ccggagcggt gttcgagcac gacgaagcga
      301 gcgccatgct ggagctgcgc gccacatacg gcatcaaccc cgagctggtg gacgagctga
      361 tggtgcgcac gctgcgcatg ggggagggcg ccaccgggcg cgccgccgcc acccgcgcgc
      421 cggtcgagat cgccgacgtc caggcggtag atgtgtacac gggcaagatg cgcgacgtgc
      481 tcgaccgtgc cggctaccga gccatgctgg cggttccgct ggtgcaggga gagcgcgtgc
      541 tcgggggcct cacggtcacg cgtcgcaccc cgggcaagtt cccgcccgag gtggtcgacc
      601 tgttgcagac cttcgcgacg cagtcgaccc tggccattca gaacgcgcgc ttgttccgcg
      661 agatcgagca gaagggccgc gagctggaga tcgccagcca gcacaagtcg cagttcctgg
      721 ccaacatgag ccacgagctg cgcacgccct tgaacgccat cctcggttac acggagctgg
      781 tggccgacgg catctacggc gcggttccgc agaagatccg ggaagtggtg gaacgggtgc
      841 agaagagcgg gcgtcatctg ctcggactga tcaacgacgt gctcgatatc tccaagatcg
      901 aggccgggca gctcaagctc tcggtcggcg attactcctt ccacgacgtg gtgcagagcg
      961 tgacgaccgc ggtggaatcc ctcgcagcgg agaagaagct cgcattgaag gtggacctgc
     1021 ctggtgatct gccggtcgcg gccggtgacg agcggcggat cacgcaggtg ctcctgaacc
     1081 tggtcggcaa cgcgatcaag ttcaccgaca ccggggaggt ggggatcacc gtgcgcgccg
     1141 cagacagcaa gttcctggtg gcggtgcgcg acaccggccc gggcatcgac gccgagcatc
     1201 agcagaggat cttcgaggag ttccagcaag tggacagttc ccccaccaag accaaaggcg
     1261 gcaccgggct ggggctggcg atcgccaagc gtatcgtcga gatgcacggg gggcatatct
     1321 gggtggaatc cacgctcggc ctgggctcca cgttcttcgt gcaactgccg ctcaggacgg
     1381 cgccgcaaga catcgcagca tcaaccagtg catcatgagc atcaggctga ccgcattctg
     1441 gttcgaagtg gcgatcggca acggccagac gcttcgcatc ggcccatcgg tgcgggcggc
     1501 acagacagga gggacgccat gagcaagcgc attctggtgg tcgaggacca ggaagacaac
     1561 cgcaggatca tgcgcgatct gctgtcggcg agcggctatg cctatatcga ggccactacc
     1621 ggcgacgaag ggttggcggc ggcgcagcgc gagaagcccg acctgatcct gatggacatc
     1681 cagctcccgg gcatggacgg ctacgaggcc acgcgccgga tcaaggccga tccggccctg
     1741 aagcacgtgc cgatcatcgc cgtgacctcg tacgcgctga gcggcgacga cgacaaggcg
     1801 ttcgcggccg gctgcgacgg gtacgtgacc aagccgttca gcccgaaggt gctgctggcg
     1861 aagatcagag agtacttgaa gtagcgccgg gaccgcctcc agcatgcacg atccgccccg
     1921 catcctggtc gtcgacgaca acgccgccaa cgtcgagatc ctgcagatgc gcctcctggc
     1981 caacggctac gaggtcgtca ccgcctcgga cggcgtggag gcgttgtccg ccacgcgccg
     2041 gcacctaccc gacctgatcc tgctcgacgt gatgatgccg aagatggatg gcatcgaggt
     2101 gtgccggcgc ctgcgggcgg acgcctcgct gcccttcatc ccgatcgtgc tggtcaccgc
     2161 gaaatccgac cccaaggacg tggtggccgc gctggaggcc ggcggggacg agtatctgac
     2221 caagccggtg gaccagacct cgctggtggc gcgcgtgaag tcgatgctgc gcatcaaggg
     2281 gctacacgac acggtgcagc agcagtcggc gcagctgaag gagtggaacg cgggcctgga
     2341 gcagcgcgtg gcgcagcagg tcggtgagct cgagcgcttc ggacgcttga agcgtttctt
     2401 ctcaccgcaa ctggtcgagt cgatcctggc gggcggggcg gaggacccgc tcaagagcca
     2461 tcggcgcgag gtggtggtgg tgttcctcga tctgcgcggc ttcaccgcgt tctccgagac
     2521 cgccgagccg gaggaagtga tgggcgtgct gcgcgaatac caccagcaca tgggcggcat
     2581 ggtgctggcg cacgagggca cgctggagcg tttcgcgggc gacggcatga tggtgttctt
     2641 caacgatccg gtgcaagtgc ccgatccggc cgcgcgcgca gtgcgcatgg cggccagcat
     2701 gcgcgagcgc ctgggcgaac tgacggcgcg ctggcgtaag cgcggcttcg cgctggactt
     2761 cggcgccggc atcgcacagg gctacgccac catcggcgcg atcggcttcg aaggccgctg
     2821 ggactacggc gcgatcggca ccgtcaccaa cctcgcctcg cggctgtgcg acgaagcgag
     2881 ccccggtcag gtcttgctgt cgcagcgggt gctgtccggc gtggaggacc tggtagaggt
     2941 ggagccggtg ggcgcgctgc agctgcgcgg cttcttgaag ccggtgcaga cgtacaacct
     3001 aatcgggatg aaagcctagg atctatcgag gtaggccacc tctcccagcc ggctacaccg
     3061 agcggcgaat cgggtctttt taaatcgcgc cttcagggcg cataaaagcg gtacttgttc
     3121 ttgccgcttt ccttggcgtg gtacatcgcc gtgtcggcgt tgcggatcag cgtgtccgcg
     3181 tctgcgccgt ggtgcggata gatgctcacg cccacggtgg gggtgatggc gagctccctg
     3241 ccctcgatcg catacggggc cgacagcgcg gcgacgagct tgcgcgccac cgcgccggcg
     3301 tcgccgactt cgcgcagatc gggcagcacg agcacgaact cgtcgccacc aagccggcac
     3361 acggtgtcga tctcgcgcac gcagcttttg gcgcgctcgc cgatctcgcg cagcagctgg
     3421 tcgcccgcgt ggtggccgta cgaatcgttc accgccttga agtggtcgag atcgacgaac
     3481 agcactccca cctgcgagcg gttgcgttgc gccgaggcga tggcctgctc caggcggtcg
     3541 cgcagcagca gccggttggg cagcccggtc agggagtcgg agtacgccag gctgtagatg
     3601 cggttctccc aggtcttgcg ctcggtgatg tcgcgcacca gcgcggcgaa cttgggcggc
     3661 ccgggcatga gcgtgatctc ggacagcgaa agctccagcg ggaacaggct gccgtcctgg
     3721 cgcctgccgg tcacttcgcg acggccgccg agcaggcggc gcatgcgctg cgccgggctc
     3781 tcctccgcgg cggccggctc cccgtcgtag tcgagctcca cggtgaggtg gcgcaggttg
     3841 gtgcgcagcg cctggtcggc gccgtagccg aacatgcgct gcgccaccgg gttgatgcgc
     3901 tcgatcccgc cttcggccga aaccacgagc atgccctcgg cggcgttgtt gaggatcgac
     3961 tcgagctggt cctggtgctc ccgccaggcg ttggcggccg ttgcggcgcc cgccgcatcg
     4021 tcttgcgccg cggccggccg cgcctcaccc gtcggcgacg gcatgagatg gcaggcgtcg
     4081 aggtcctgca ccgtgccggc catgcgcagc gcgttgccgt cgcggtcgcg gccgatcacc
     4141 cgcccgcgcg ccaggagcag cttccaccgg cccgacccca ggcgcacgcg cagcgatcgc
     4201 tcgaacggct ccgagggcac gcgcaagtgg gcgttgagtc cctcggcgag gctcgcgctg
     4261 tcctcggggt gggccagatg ctggcacgga taggtgtcct ggggcagcgt gcccgggggg
     4321 acctgcagca tgccggcgag ctcgtcggag tagaacacgc gcccgccggg gatgtcccac
     4381 tcccacagcg ccagcgccgc cgattcgaga gcgagcgcga gacgctgtcg gctctcgttg
     4441 accaggtcga tcagggccgg gttgtccagc gccccaagcg cttgcctgga cacgaaatcc
     4501 ggatcggcgc cggcctgcag cgcgcgcagg cgcgcgacca gctcgtcgcg actgaggttt
     4561 tccaccgatt gcattcgccg cggctcctcc cgagtccccg cccgcgcaga ttgcagcggc
     4621 gaaagtatag agcggtcgcg ccgaattttg aaaagctact tgttactttc tgctaacagg
     4681 ttgatgcgac gttggaattc gaacgtcaaa cgacgccgcg ggccggccgc gcgcaggcgc
     4741 gcggctataa tcgcgctgct gcgtcgaagg agcgccattg tttcttccca tcgcggacga
     4801 gcccaatcct cgtcactttc cggccatgac ggtggcgttg atcgccatca atgtcgccgt
     4861 ctatctgctg gtcagcctgc cgcttggcat gcggccgccc gaccccaccg acccggcgct
     4921 cgccgagtac ctgcgcgcgc tgctgccgca cctgcccccg ggcacctcgg cgcgggaagt
     4981 gctgcagcag atgagcgcct acgacctggt agtgtacggc tggggcttcc ggcccgcggc
     5041 gccgagcctg gtgacgctgg tcaccgccat gttcctgcac ggcgggctga tgcacctctt
     5101 cgggaacatg ctctacctgt ggatctacgg caacaacgtc gagcaccgcc tcggcatgcc
     5161 ggcgtacctg ttctggtatc tcgccaccgg catcgccgcc acgctgttcc acacgctgtt
     5221 caacgtcggc tcgccgatcc ccctggtggg ggcatcgggg gcgatctcgg gcgtgctcgg
     5281 tttctacctg atctggttcc cgcaccacac cgtgaaggtg tgggtgttcc tgttcccgat
     5341 ctacatgggg atggtgcaca tcagcgcgac catcgtgctg gtggtctacc tgctgatcga
     5401 caacctgctg ccgttcctgg cggcgcccgc cggcggcatc gcgcacggcg cgcacatcgg
     5461 cgggttcatc gccggcgcga tggtggcgtt gatcctgcgg cgtaagcgtc ggtagcgcag
     5521 cccggatgcg aaagcggctc acgtagcccg gatgcagcga agcggaatcc gggggcgaga
     5581 aaggccctgt catccccgga ttccgctacg ctgcatccgg gctacgctag ttacgcccgt
     5641 tacgctggcg atgtcacgct cgcctcagcg aatcgcggat gtcgcgcaac agtaccacct
     5701 cctccggcgg agcagcgggc gcggggggcg gagcctggcg cttgagccgg ttgaactgct
     5761 tgatcatcag gaagat
//
LOCUS       DASPGE010000172         3563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_163346, whole genome shotgun sequence.
ACCESSION   DASPGE010000172 DASPGE010000000
VERSION     DASPGE010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163346"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VD791_05445"
     CDS             <1..322
                     /locus_tag="VD791_05445"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HXV07451.1"
                     /translation="PCEIEAGQARFAGVPLAAAARAAPGARTEIGVRPEFVRFADEGV
                     PVRVARVADAGRHQIVETRHGDTVIRVLLKEGERVPGENARLAFDPAHTRLYVDGWMA
                     GEAA"
     gene            319..1188
                     /locus_tag="VD791_05450"
     CDS             319..1188
                     /locus_tag="VD791_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXV07452.1"
                     /translation="MSAKPVNPRAWWLVLPVLVLVAFNAVIPLMTVVNYSVQETFGNN
                     QFFFEGVKWYQDVVNSARFHEALKRQLLFTAIILAIEVPLGVAIALAMPRKGAWASLC
                     LVLMSMPLLIPWNVVGAMWNIFALPDIGLLGWTLNKIGLDYNYTRQPFAAWATLIAMD
                     VWHWTSLVALLAYAGLSAIPDAYYQAAKIDGASPLAVFRHVQLPKMKHVLTIAVLLRF
                     MDSFMIYTEPVVLTGGGPGNATTLLSIDLVKIALGQFDLGPAAAMSLIYFLIILLLSW
                     VFYRVMTRDEEPS"
     gene            1185..1985
                     /locus_tag="VD791_05455"
     CDS             1185..1985
                     /locus_tag="VD791_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXV07453.1"
                     /translation="MRRVKWLVPALYIAFLMLPIYWLLAMSFKTTNEILGGFTLWPQQ
                     FTLENYAKIFTDPTWYLGYVNSLTYVVINTVLSVAVALPAAYAFSRYRFLGDKHLFFW
                     LLSNRMAPPAVFALPFFQLYSAIGLFDTPIAVALAHCLFNVPLAVWILEGFMSSVPRE
                     LDEMAYVDGYPFWRFFLRIFVPAIAAGIGVAAFFCFMFSWVELLLAKTLTSVDAKPIA
                     ATMTRTASTSGYELGLLAAAGSLTIIPGAFVIYFVRNYIAKGFALGRV"
     gene            2009..2290
                     /locus_tag="VD791_05460"
     CDS             2009..2290
                     /locus_tag="VD791_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2160 domain-containing protein"
                     /protein_id="HXV07454.1"
                     /translation="MALSWMAWTWQTATFFAVIALLLAGMGLWERRVPGGAPRRGLLG
                     IHTTRGDRLFISLLLAAYIHLLWLAATRLPLWGATVVALIAAVLVFRKV"
     gene            2425..2985
                     /locus_tag="VD791_05465"
     CDS             2425..2985
                     /locus_tag="VD791_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HXV07455.1"
                     /translation="MPLPVSEIERELVHTRRVRYEGYKRADGLWDIEAHITDVKNHDF
                     TLKTGVRRAGLPVHDMVVRVTIDRKFNVVDARAVSDAVPYPGGCEAAAPEYAKLVGLN
                     LLKKFRDGLKERLGGVRGCTHITEMLAALPTAAIQTFAGEMREDRMDERKPFQLDHCL
                     AMDTTGETVKKWYPQWYAGSKEKAGA"
     gene            2982..>3563
                     /locus_tag="VD791_05470"
     CDS             2982..>3563
                     /locus_tag="VD791_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXV07456.1"
                     /translation="MKIHEYQGKEIFRRYGVPTPRGVPCFSVDEAVAAAKTLGGKVWV
                     VKAQIHAGGRGKGGGVKVAKSLDEVRAHAKKILGMQLVTHQTGPGGQKVRRLLVEEGA
                     DIQKELYVGLVVDRGSQRVALMASSEGGMDIEEVAAKTPERIHKVIVDPQAGLAETEA
                     RDVARRLGVPEGALGDAARLLQGLYRCFWETDAS"
BASE COUNT          514 a         1235 c         1217 g          597 t
ORIGIN      
        1 gccctgcgag atcgaggcgg ggcaggcgcg cttcgccggc gtgccgctgg cggccgccgc
       61 gcgcgccgcg cccggcgcgc gcaccgagat cggcgtgcgg cccgagttcg tgcgcttcgc
      121 cgacgagggc gtcccggtgc gcgtcgcgcg tgtcgccgac gccgggcgcc accagatcgt
      181 cgagacgcgc cacggcgaca cggtgatccg cgtgctgctc aaggaaggcg agcgcgtgcc
      241 cggggagaac gcgcgcctcg ccttcgaccc ggcgcacacg cggctgtacg tggacggctg
      301 gatggccggg gaggcggcgt gagcgccaag cccgtgaacc cgcgcgcctg gtggctggtg
      361 ctgccggtgc tggtgctggt ggccttcaac gccgtcatcc cgctgatgac ggtggtgaac
      421 tactcggtgc aggagacctt cggcaacaac cagttcttct tcgagggcgt gaagtggtac
      481 caggacgtgg tcaactcggc gcgcttccac gaggcgctca agcgccagct gctgttcacc
      541 gcgatcatcc ttgccatcga ggtgccgctc ggcgtggcga tcgcgctcgc catgccgcgc
      601 aagggcgcct gggcgtcgct ctgcctggtg ctcatgtcga tgccgctgct cattccctgg
      661 aacgtggtcg gcgccatgtg gaacatcttc gcgctgcctg acatcggcct gctgggctgg
      721 acgctgaaca agatcggctt ggactacaac tacacgcgcc agccgttcgc cgcctgggcg
      781 acgctgatcg ccatggacgt ctggcactgg acctcgctcg tcgcgctgct cgcctacgcg
      841 gggctctcgg cgatccccga cgcctactac caggcggcga agatcgacgg cgcctcgccc
      901 ctggcggtgt tccgccacgt gcagctgccg aagatgaagc acgtgctgac catcgcggtg
      961 ctgctcaggt tcatggacag cttcatgatc tacaccgaac cggtagtgct caccggcggc
     1021 gggccgggca acgccaccac gctgctgtcg atcgatctgg tgaagatcgc gctcggccag
     1081 ttcgacctcg ggccggcggc ggccatgtcg ctcatctact tcctcatcat cctgctgctc
     1141 tcctgggtgt tctaccgcgt gatgacgcgc gacgaggagc cgtcatgagg cgcgtgaaat
     1201 ggctggtgcc cgccctctac atcgccttcc tgatgctgcc gatctactgg ctgctcgcca
     1261 tgtcgttcaa gaccacgaac gagatcctcg gcggcttcac gctgtggccg cagcagttca
     1321 cgctggagaa ctacgccaag atcttcaccg acccgacctg gtacctgggg tacgtcaatt
     1381 cgctcaccta cgtggtgatc aacacggtgc tgtcggtggc ggtggcgctg cccgcggcct
     1441 acgctttctc gcgctaccgc ttcctcggcg acaagcacct gttcttctgg ctgctttcca
     1501 accgcatggc gccgccggcg gtgttcgcgc tgcccttctt ccagctgtac tcggcgatcg
     1561 gcctgttcga cacgcccatc gcggtggcgc tggcgcactg cctgttcaac gtgccgctcg
     1621 cggtgtggat cctggaaggc ttcatgtcga gcgtgccgcg cgagctcgac gagatggcct
     1681 acgtcgacgg ctacccgttc tggcgcttct tcctgcgcat cttcgtgccg gcgatcgccg
     1741 ccggcatcgg cgtggccgcc ttcttctgct tcatgttctc ctgggtcgag ctgctgctcg
     1801 ccaagacgct cacctcggtg gacgcgaagc ccatcgccgc caccatgacg cgcaccgcga
     1861 gcacctcggg ctacgagctg ggcctgcttg ccgcggccgg gtcgctcacc atcatcccgg
     1921 gcgccttcgt catctacttc gtgcgcaact acatcgcgaa aggattcgcg ctcggccggg
     1981 tctgacccgg cgcgcgcaac ccccgcccat ggcgctctcc tggatggcat ggacctggca
     2041 gacggcgacc ttcttcgcgg tcatcgcgct gctccttgcc ggcatgggcc tgtgggagcg
     2101 ccgcgtgccc ggcggcgcac cgcggcgggg cctgctcggc atccacacca cgcgcggcga
     2161 tcgcctgttc atctcgctgc tgctcgccgc ctacatccat ctcctctggc tggcggcgac
     2221 gcgcctgccg ctctggggcg cgaccgtcgt cgcgctgatc gccgcggtgc tcgtgttccg
     2281 caaggtctag ccggtcatgg gtctcctcct tccccacggg gtacccggcg aagctgcgcg
     2341 gaccacgggc actgcggcga ccgtttcatg acctaggtca agccggcggt cgggccgcgc
     2401 gccgatgata aactctcgcg cgccatgcca ctccccgttt ccgagatcga gcgcgagctc
     2461 gtgcacacgc gccgcgtgcg ctacgaaggc tacaagcgcg ccgacggcct gtgggacatc
     2521 gaggcccaca tcaccgacgt caagaaccac gatttcacgc tgaagaccgg cgtgcgccgc
     2581 gccggcctgc cggtgcacga catggtggtg cgcgtcacca tcgaccgcaa gttcaacgtc
     2641 gtcgacgccc gcgcggtgtc cgacgccgtt ccctacccgg gcggctgcga ggccgcggcg
     2701 cccgagtacg cgaagctcgt cggcctgaac ctgctgaaga agttccgcga cgggctgaag
     2761 gagcgcctgg gcggggtgcg gggctgcacg cacatcaccg agatgctggc ggcgctgccg
     2821 accgcggcca tccagacctt cgccggcgag atgcgcgaag accgcatgga cgagcgcaag
     2881 cccttccagc tcgatcactg cctggccatg gacaccaccg gcgagaccgt gaagaagtgg
     2941 tatccgcagt ggtacgcagg cagcaaggag aaggcgggcg catgaagatc cacgagtacc
     3001 agggcaagga aatcttccgc cgctacggcg tgcctacgcc gcgcggcgtt ccctgcttca
     3061 gcgtcgacga agccgtggcg gcggcgaaga cgctgggcgg caaggtgtgg gtggtgaagg
     3121 cgcagatcca cgccggcggc cgcggcaagg gcggcggcgt caaggtggcg aagtccctgg
     3181 acgaggtgcg cgcgcacgcg aagaagatcc tcggcatgca gctggtcacg caccagaccg
     3241 gccccggggg gcagaaggtc cggcggctgc tggtggagga gggcgcggac atccagaagg
     3301 agctttacgt aggcctggtg gtggatcgcg gctcgcagcg cgtcgcgctc atggcctcct
     3361 ccgagggcgg catggacatc gaggaggtgg cggcgaagac gccggagcgc atccacaagg
     3421 tgatcgtcga tccgcaggcg ggcctcgccg agaccgaggc gcgcgacgtg gcgcgcaggc
     3481 tcggcgtgcc ggagggcgcg ctcggcgacg cggcgcgctt gctgcagggc ctgtaccgct
     3541 gcttctggga gaccgatgcc tcg
//
LOCUS       DASPGE010000173         4786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_749177, whole genome shotgun sequence.
ACCESSION   DASPGE010000173 DASPGE010000000
VERSION     DASPGE010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749177"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VD791_05475"
     CDS             <1..365
                     /locus_tag="VD791_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HXV07457.1"
                     /translation="NCAACHVPALKTGPKPALRAAADLTIHAYTDLLLHDMGEELADH
                     RPDFKADGREWRTAPLWGIGLLRTVNGHSDLLHDGRARDVTEAILWHGGEAEKSREAF
                     RTLGKADRDALVKFVESL"
     gene            complement(494..904)
                     /locus_tag="VD791_05480"
     CDS             complement(494..904)
                     /locus_tag="VD791_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXV07458.1"
                     /translation="MSAPAVAGTQTLHETRRGPGACAPGSRRRIAAALLTAALGAGIA
                     AAQEAPPAPQNPYLDQKEALDEGERIYRARCIGCHFRGGGRGPNVFRTKLTLVQFVEV
                     VAKGGGPGMPAWGGTLSGEDILKLHAFVMSRDRL"
     gene            complement(901..2736)
                     /locus_tag="VD791_05485"
     CDS             complement(901..2736)
                     /locus_tag="VD791_05485"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HXV07459.1"
                     /translation="MLRNSCLAAAALAAVVSVAPAGAAEVSYQDLLNSGRNTRDWLSY
                     GRTYDAQRYVPLTQITPQNVRRLQAAWVFATGGDNRGLQATPLVHDGVIYLSADQSRL
                     FAIDARTGMPKWRYDPKIAKDVERVYCCGSNNRGAALLGNLVYIGTMDARLVALNKDT
                     GAVAWEVKVADWEHGYSITGAPLAVKDMVLTGVAGGEFGIRGFVAAYDARTGAQRWRR
                     HVIPGPGEPGNDSWPGDTWKHGGGSTWTTGVFDPGMNTVFWQVGNAGPWNCQVRKGDN
                     KWTAGTVALNADTGEIQWGFQYTPWDCWDYDSVSTPVLADLQLGGKAVKALFHYDKNG
                     YFYALDRSTGKFLYGEPIVPGINWTKGLDPITGRPNVNPEMIAQSGGPEIAPIIPSLE
                     GAIDWQPLAFDPGRRTMFFMSNNWAMGYKFWAEDKFAPPTKGEWYLGADYQQYISHGE
                     NTGNFVAFDVANRKVAWRVVSPAPFWSGALVTSSGLVFAGDMRGYFLAIDAATGKVLW
                     YFPTGSGIIGSPISYQLDGVQYVAVPSGGMGGDMGFYYTEPKAGNLWVFALDGRRPPM
                     GKDTNLVSIPGSTPQVGEPGTTLGGRVLPGYGYPEKPEREPIKKQ"
     gene            2958..4001
                     /locus_tag="VD791_05490"
     CDS             2958..4001
                     /locus_tag="VD791_05490"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXV07460.1"
                     /translation="MVRFRFDVTAACVVALLAAPPVAAQKVRLNLTSAFPSTLTLVGE
                     AAPKFAHRVQRASGGTLEFKVHEPGAIVPPLQAIQAVSRGSVDAAWSPAGFFSGTDSS
                     FAMFTSVPFGPGMGEYLAWIYHGGGIELMNEMFGKHGIHAIPCAMTPPEASGWFRKEI
                     KTVQDLKGLKMRFFGMGAKVMDRLGVSTQLLAPGDIFQSLQLGTIDATEFAMPSMDLK
                     FGFHQVAKYYYFPGWHQQTAMLLLNINDKKWAALSDQHKAIIELACGETLMDMIAEGE
                     ATQWKAMKEMRDKHGVKIMRWPPEIMKAYEKAWNEVVAEESAKNPNFKRIYDSYAKFR
                     AEYALWRDYGYLQ"
     gene            4018..>4786
                     /locus_tag="VD791_05495"
     CDS             4018..>4786
                     /locus_tag="VD791_05495"
                     /inference="COORDINATES: protein motif:HMM:NF026200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HXV07461.1"
                     /translation="MPAKGPRADTASPGPRRFVSRQPLPALDAARAQINRLWLADEAR
                     CVQDLIPHARLDRQACARVRARAQALVQAVRAQRVSTGGIDAFLREYDLSSQEGVVLM
                     CLAEALLRIPDATTADRLIEEKLRQGRWEEHLGGTSSLFVNASTWGLMLAGRLLRLDD
                     EAARDLSAYMGRLMARTGEPVVRQALRHAMRVLGRQFVMGRTIDEALQRAAGEEHRAY
                     RHSYDMLGEAALTAADAQRYFSSYYAAIEAVGAAAQGA"
BASE COUNT          743 a         1631 c         1652 g          760 t
ORIGIN      
        1 caaactgcgc cgcctgccat gtccccgcgc tcaaaaccgg cccgaagccc gcgctgcgcg
       61 cagcggccga tctcaccatt cacgcctaca ccgatctgct gctgcacgac atgggcgagg
      121 agctcgccga ccaccgcccc gatttcaaag ccgacggccg ggaatggcgc actgcgccgc
      181 tgtggggcat cgggctgctg cgcaccgtca acggccacag cgacctgctg cacgacgggc
      241 gcgcgcgcga cgtcaccgag gcgatcctgt ggcacggcgg tgaagcggaa aaatcgcgcg
      301 aagcctttcg cacgctgggc aaggccgatc gcgacgcgct ggtgaagttc gtcgaatcgt
      361 tgtaggcggg actcatacca caccgccccg ccggctgcgc cggcaccccg cctcgtgaga
      421 ggcggggagc agttggttcc cctcctttgc taaggagggg tggcgcgcga agcgcgacgg
      481 ggtggtcggt tttctacaac cggtcgcgcg acatcacgaa cgcgtgcagc ttgaggatgt
      541 cctcgccgga caaggtgccg ccccaggccg gcatgccggg accgccgccc ttggcgacga
      601 cctcgacgaa ttgcaccagg gtgagcttgg tgcggaagac gttcgggccg cggccgccgc
      661 cgcggaagtg gcaaccgatg cagcgcgcgc gatagatccg ctcaccctcg tcgagcgctt
      721 ccttctgatc gaggtacggg ttctgcggcg cgggcggcgc ttcctgcgcc gccgcgatac
      781 cggcgccgag cgcggccgtc agcagcgcag ccgcgatacg gcggcgcgag ccgggtgcgc
      841 atgctcccgg cccgcgtctc gtttcatgca gcgtttgcgt gcccgccacc gccggcgcgc
      901 tcactgcttc ttgatcggct cgcgctcggg tttctcggga tagccgtagc cgggcagcac
      961 gcggccgccg agcgtggtgc cgggctcgcc gacctgcggg gtggaaccgg ggatactgac
     1021 caggttggtg tccttgccca tcggcggcct gcgcccgtcg agcgcgaaca cccacagatt
     1081 gccggccttc ggctcggtgt aatagaagcc catgtccccg cccatgccgc ccgagggcac
     1141 ggccacgtat tgcacgccgt ccagctgata ggagatgggg ctgccgatga tgcccgagcc
     1201 ggtggggaag taccacagca ccttgccggt ggcggcgtcg atcgccagga agtagccgcg
     1261 catgtcgccg gcgaagacca gcccgctcga ggtgacgagc gcgcccgacc agaacggcgc
     1321 cgggctcacc acgcgccagg ccactttccg gttggccacg tcgaaggcga cgaagttgcc
     1381 ggtgttctcc ccgtgcgaga tgtactgctg atagtccgcg cccaggtacc actcgccctt
     1441 ggtgggcggc gcgaacttgt cctcggccca gaacttgtag cccatggccc agttgttgga
     1501 catgaagaac atggtgcgac ggccgggatc gaaggcgagc ggctgccagt cgatggcgcc
     1561 ttccaggctg gggatgatgg gcgcgatctc gggtccgccg gactgggcga tcatctccgg
     1621 gttgacgttc ggccggccgg tgatcggatc gagccccttg gtccagttga tgcccggcac
     1681 gatcggctcg ccgtacagga acttgccagt gctgcggtcg agcgcgtaga agtagccgtt
     1741 cttgtcgtag tggaacagcg ctttcaccgc cttgccgccg agctgcaggt cggccagcac
     1801 cggcgtgctg acgctgtcgt agtcccagca atcccagggc gtgtactgga agccccactg
     1861 gatctcgccg gtgtcggcgt tgagcgcaac ggtacccgcg gtccacttgt tgtcgccttt
     1921 gcgcacctgg caattccatg gtccggcgtt gcccacctgc cagaacacgg tgttcatgcc
     1981 cggatcgaac acgcccgtgg tccaggtgga gccgccgccg tgcttccagg tgtcgccggg
     2041 ccagctgtcg ttgccgggct cgcccgggcc ggggatcacg tgccggcgcc agcgctgcgc
     2101 gccggtcctg gcgtcgtagg cggcgacgaa accgcggatg ccgaactcgc cgccggccac
     2161 gccggtgagc accatgtcct tcaccgccag cggcgcgccg gtgatgctgt agccgtgctc
     2221 ccaatcggcc accttgactt cccaagccac cgccccggtg tccttgttga gcgcgaccag
     2281 gcgcgcgtcc atggtgccga tatacaccag gttgcccagc agcgcagcgc cgcggttgtt
     2341 cgagccgcag cagtacacgc gttccacgtc cttggcgatc ttcgggtcgt aacgccactt
     2401 cggcatgccg gtgcgcgcat cgatggcgaa caggcgcgac tggtcggccg acagatagat
     2461 gacgccgtcg tgcaccagcg gcgtggcctg caggccgcgg ttgtcgccgc cggtggcgaa
     2521 cacccacgcc gcctgcaggc gccgcacgtt ctgcggcgtg atctgcgtca gaggcacgta
     2581 gcgctgggcg tcgtaggtgc gcccgtagct caaccagtcg cgcgtgttgc gacccgagtt
     2641 gagcaggtcc tgatagctga cctcggcggc gcccgcaggc gccacggaca cgaccgccgc
     2701 cagcgcggcg gccgccaggc atgagttgcg cagcatgatc tcctcctcgg ccatgttgta
     2761 gagtgtgtgc aggctttacc gccgagccag aatagagcag cgcatgcgcc agggcaagcg
     2821 gcatcgtcgt gcgcgccgct gttcgacacc tggcaagact ggacaagcgc cgggaatgcg
     2881 cctttaatgc ctgaacaatg cggccgccgg cgatgcacgg cgccgctgat cctgcagggc
     2941 atccagagga gaaggccatg gtccgattcc gcttcgatgt gactgcggcc tgcgtggtgg
     3001 cgctgctggc cgcgccgccg gtcgcggcgc agaaggttcg cctcaacctg acgagcgcct
     3061 tccccagtac gctcacgctg gtgggcgagg ccgcgccgaa gttcgcgcac cgcgtgcagc
     3121 gcgcgtcggg cggcacgctc gagttcaaag tgcacgagcc gggcgcgatc gtgcccccgc
     3181 tgcaggcaat ccaggcggtg tcgcgcggct cggtggacgc cgcctggtcg cccgccggtt
     3241 tcttctccgg caccgattcg tccttcgcga tgttcaccag cgtgcccttc ggcccgggca
     3301 tgggcgagta cctcgcctgg atctaccacg gcggcggcat cgagctgatg aacgagatgt
     3361 tcggcaagca cggcatccac gccatcccct gcgccatgac cccgcccgaa gcgtcgggct
     3421 ggttccgcaa ggagatcaag acggtgcagg acctgaaggg cctgaagatg cgcttcttcg
     3481 ggatgggcgc gaaggtgatg gaccggctcg gcgtgtccac gcagctgctc gccccgggcg
     3541 acattttcca gtcgctgcaa ctgggcacga tcgacgccac cgagttcgcc atgccgtcga
     3601 tggatctgaa gttcggcttc caccaggtgg ccaagtatta ttacttcccg ggctggcacc
     3661 agcagaccgc catgctgctg ctcaacatca acgacaagaa atgggcggcg ctgtccgacc
     3721 agcacaaggc gatcatcgag ctcgcctgcg gcgagacgct gatggacatg atcgccgagg
     3781 gcgaggccac gcagtggaaa gcgatgaagg agatgcgcga caagcacggg gtcaagatca
     3841 tgcgctggcc gcccgagatc atgaaggcct acgagaaggc gtggaacgag gtggtggccg
     3901 aggagtcggc caagaacccc aacttcaagc gcatctacga ctcgtacgcc aagttccgcg
     3961 ccgagtacgc gctgtggcgc gattacggct acctgcagta gcgccgatct gaagcgcatg
     4021 ccggccaagg gtccacgggc cgacactgcg agccccggtc cgcgccgctt cgtctcgcgc
     4081 cagccgctgc ccgcgctcga cgccgcgcgc gcgcagatca accgcctgtg gctcgccgac
     4141 gaggcgcgct gcgtgcagga cctcattccc cacgcgcggc tcgatcggca ggcgtgcgcg
     4201 cgtgtgcgcg cgcgggcgca ggcgctggta caggcggtgc gtgcgcagcg cgtcagcacc
     4261 gggggcatcg atgcattcct gcgcgagtac gacctttcgt cgcaggaggg cgtggtgctc
     4321 atgtgcctgg ccgaagcgct gctgcgcatc ccggacgcca ccaccgccga ccggctgatc
     4381 gaggagaagc tgcgccaggg ccgctgggaa gagcatctgg gcggcacctc gtcgctgttc
     4441 gtcaatgctt cgacctgggg cttgatgctg gctgggcggc tgctgcggct ggacgacgag
     4501 gccgcccgcg acctgtccgc ttacatgggg cggctgatgg cacgcaccgg cgagccggtg
     4561 gtgcgccagg cgctgcggca cgcgatgcgc gtgctcggcc gccagttcgt gatggggcgc
     4621 accatcgacg aggcgctgca gcgcgccgcc ggcgaagagc accgcgccta tcgccattct
     4681 tacgacatgc tcggggaggc cgcgctcacg gccgcggatg cgcagcgcta cttctccagt
     4741 tattacgccg cgatcgaggc ggtcggcgcg gccgcgcaag gcgcgc
//
LOCUS       DASPGE010000174        10349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_46796, whole genome shotgun sequence.
ACCESSION   DASPGE010000174 DASPGE010000000
VERSION     DASPGE010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10349
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46796"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2246
                     /locus_tag="VD791_05500"
     CDS             <1..2246
                     /locus_tag="VD791_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07462.1"
                     /translation="QLRDLARRLNGILSDTLGLNQAVIKDEAREVFRAVRQELLVGVG
                     VMSPRSAGIHLALASLIGRLEQEVFDAWPTVDLNPDRLAHEAFQALQRTGFEKLRAQL
                     ACILEKARATLAAGGALIDLAKPDAFAGSVGAQARAPLSGDKYCWYASWLYATRRQGS
                     GTALVPCYPADEVWLSEDRRQLFLRVASGEDEVLHEVASGTINWYDAPQFTTASGPEC
                     FTFGAVSPQFMETWTHVFAAFVEFVKGLLHIINCATSPKEYASNIPFIVWDLTKTVSV
                     ALGEAPLPSLIAQKAGAGVGASQVLFNLLPILMVLGGSFEGIHTKTTGGNIFLQWVTL
                     LGGDALNAFSNHSIVSTANDALLSFWTLINQTGPAGPPDGDDTRPRNREYGGPLIGLV
                     NTGANALMMKYIIPREDYAHPFEGQNVKNFLIWWLAGSTLAGVTGGLVGTLLVWTLSR
                     TYDWPQFGEEIGLGALRSFLGFVVTQYALMEGDTDGGKYNPKLDPEGNPYSPARQPFA
                     GYPPADTSPYKLPYERGVSMFAGQANQGMFSHMRFNWLPQIYAYDFAHDFGDEILASR
                     GGTVVDFFDWIEDNTDPDGAAQTAALTASEAVMGASWRSSAESWNFITIRHDTNVADH
                     DKDVNGTATTTYATYGHGKKGSVREVFNTKGVAPTAIIGRTVVQGEVIMLADDTGVSF
                     HNHLHMHVRGGPAPPASPPATPTPIADASLNQYTLPFVFREAKHVIARDGVLRNLTWY
                     KSDNEKF"
     gene            2252..3328
                     /locus_tag="VD791_05505"
     CDS             2252..3328
                     /locus_tag="VD791_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07463.1"
                     /translation="MDLGLPFLSDSSPAPGFRPGCKITQGAAASGGGGLLDAVASLLS
                     APQADPWAENLRSVEIVQGPAPFLDTCRLLLSRTSTTPQLAVDDELGVQLGFEDSLTT
                     VFTGKVALLGATRDQGLEAVLASPAHLLAQSRQNASFEQRSLGDLLRSWAGEAQVTPG
                     DLDTGPSFPFLAVDDRRSAWEWIGVLARLAGLLAWVDGEGKLQCKQPAGAPARSYRYG
                     QDLLWLRSTERVANLGEVTVSGEGAAGSQGAKAWSWLAKSADSMQASAGDGAPKRLYQ
                     EGALREHAAVDAAANGLSERAQALRSIVEVTVPGSPEVGVGALFELADCPDGRGDGTY
                     VALGLRHRYAKARGFVTEISGAAT"
     gene            3325..3936
                     /locus_tag="VD791_05510"
     CDS             3325..3936
                     /locus_tag="VD791_05510"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HXV07464.1"
                     /translation="MNDLYETLRKLVRQELATLRVAELATVQEAFPADPANYECTVVL
                     RDSQLVLKNVPLATPRKGLAGVPDVGDLMLVQFVGGDLNRPVIIGSLYNDEDRPPENK
                     EQQLVLHMPIDADPDSALRLELNEASPMSLKLNIGPALKLTLQDDDPVVSLDVGGNAK
                     LTVDRSGAVKLESNGELAIKAGANLKIEAGGELKLKGAVINLN"
     gene            3945..4337
                     /locus_tag="VD791_05515"
     CDS             3945..4337
                     /locus_tag="VD791_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HXV07465.1"
                     /translation="MGQPAAKQGDQVMATDIHIEMVPTPGGPVPTPLPNPFTGMLDGS
                     LSSDVKIEGKPAAVVGSTATNTPAHIFKSGPFQKPPANRATIQLGSSTVLINGKPAAR
                     NGDTAITCNDPADLPAGTVVAVGTVLIG"
     gene            4348..4821
                     /locus_tag="VD791_05520"
     CDS             4348..4821
                     /locus_tag="VD791_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HXV07466.1"
                     /translation="MPVRAEKTEHLLRDLAIEAVHRELRPVYTVLDPKTPAPNGRGRV
                     WDLVPRAGKENLAQAVIMRLLTPRGELAALGHPEYGSRIHELIGRENTGTQRNLLKLH
                     VLEALQFEPRVAKVVELNVVPSPGTRSTVDVLLRVQVAGAAELVQIGPFSIELAV"
     gene            4846..6603
                     /locus_tag="VD791_05525"
     CDS             4846..6603
                     /locus_tag="VD791_05525"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HXV07467.1"
                     /translation="MSFRRKTYPEVSDHLLNRLLGGVSAEAHAYPPPKSAREPFAHPL
                     EKAPVTAITSVYGLLNGESSVFARDVDYELSADGAQMVWKTGGRRPDAGSVVEINYLP
                     KQRESGLNDLYPGSVVRTLLEAVALETAGLYAQMETVYRSGFIDTADGGALDHVVSVL
                     GLQRVKAGRNVAEIELTRTKNTRGEILVPAGTRVLTADGGIEYETLADITLADGQPGA
                     KGSARDLVETNDAVPAGSLTLLAKPIAGIDSVTNPAPSTRLDRDETDAELRTRAKSFL
                     AGSERGTLGAINAAIARQGILADVDDTEPGLIHILFHDDQLAPEQRKRLEDAVHAVRP
                     AGVAVQFEYGQPPLAVDLEIRLTSAPGLLETDLKHIQQDVRARVTDYFAKLPTKAAGS
                     VSKLIGLTMGVEGVEDVAIVSAVAGGDDVLDTAKGELAIAGTPTQLGALNIVDPALAT
                     LLVALVRYTTEQKIPDQAAIQTALQTAVSYLNELNAAPAAPLQKRTLSWGKLTLATPL
                     PDLTAATLAAYDANPGAFTLPTAAQRAPYTLQFVFTRPTGVSQVLDSEAVPALVLAPF
                     ERLSLAKVSTEVKPKGASA"
     gene            6600..9011
                     /locus_tag="VD791_05530"
     CDS             6600..9011
                     /locus_tag="VD791_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07468.1"
                     /translation="MSAHDRLRDLLPSLYRPEPGEGTLVNRMLLASGIVFDGVAVQLQ
                     HVLRAHWFDIADKATWDAHYQTARRERGLPAANVRNVKDLKEILQYPYVADLARVCAL
                     LELPPWLEPTDLRESVEEYRQRVRDVLDAYRLGLTTLAALRRLTEAALPEDMGAPLPQ
                     QRWPFAIEEPVALTRSRTQILVPDAQEGDLVSPLFRWTLGDVQGTPTVYIQGVAPSGE
                     LIAATEQPAIERFTPGETPAGTALAYLGTLAQGQTLRLSPARRTWLVLAGALQASPEE
                     SAANAAQDPSANGPWTALSNAPTGQVRLLARALDNTLWLALEDGGNWSLHRYDGAKFD
                     AVTQGAPAGPYLCLRAQGAGVYLGAQQGLFRCALFPDANQPFALGAVTAVPEAVQALA
                     VLPDGQLACAGGNGLARLAPDDTLADRLLPGVGLRAVQADRDQLYLATAQALLLWDHE
                     RWFRYEGAQLSEGAADWVALDAGEVAAAVSPLPAVNAIAVTPDGSVWLGTARGLARYY
                     VHGDRTTLLEAFPDLGSGEIRALTVDERGMLWIAADDGLFRYDGRDLAQYDFAEKRWV
                     QLGPADAVYPDGISAAARGHWRFDGAQDRWERFDAAAARFTTFPLSKRAGNSAAAQAL
                     LVADSLRAELGQFDGNEFSDSQPVAASQLALRIKPDETRIVAGGLPALPGGAGTWRYL
                     QLEPANLAPPSGRPWWSREGRLFLPPEWQAPWPGHFRNEASAWHADGHFDDAVFVYPP
                     SARVWMEYPVSPVVGVRVRLFRRMPDQAVDPALIDRVWQLLARAKAAGVPLQLAVEGM
                     VVKGE"
     gene            9015..>10349
                     /locus_tag="VD791_05535"
     CDS             9015..>10349
                     /locus_tag="VD791_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07469.1"
                     /translation="MSLQDAITQIQGLAPKAAGHLVRPQDWNALIAALGEFGAAITTN
                     TQDLATLQTQVQQILTQLGQVVGQVQVLDSRVDELEQSIQPLLGNYLVTLSCDRLTYA
                     SGEICELTANVTDLTGQALAAPFPWVDFVAAWGRLRAVAGFNSRAGSGDNSVSVQVNA
                     QGVAKVQLRAEHSEGFSEIEEAQVSAVMQMQVPGQNKSVAQVFMGAPTPSDAGSKSAY
                     KVLHAEYERQDSIALRAYADTYHVRTPEWSLAPLGPNYWTQWHDYRATVIAQAQPDAA
                     PTTPDNARGTASIQVTFRDWLWPWTLDYLEDLHVLQDQVFEQYQPLFQFADVYDKFQQ
                     KVDGDLPKYGVIGRKKYLSAAQKAIELINPGSDPIKQNARQQIKQAVSAQESNEIYAG
                     GKAAAGAPVMGIYLGQGKSTDDVRQQVKQVDQKVEETVGVQAAVNVLEGRMQS"
BASE COUNT         1710 a         3561 c         3569 g         1509 t
ORIGIN      
        1 cgcaactgcg cgacctggcg cgccgcctca acggcatcct gtccgacacg ctgggcctga
       61 atcaggccgt gatcaaggac gaggcgcgcg aggtgttccg ggccgtgcgc caggagctgc
      121 tggtcggcgt gggcgtgatg agcccgcgct cggccggcat ccatctggcg ctcgcttcac
      181 tgatcggccg cctggagcag gaagtattcg acgcgtggcc gacggtggac ctcaacccgg
      241 accggctggc gcacgaggcg ttccaggcac tgcagcgcac cggcttcgag aagctgcgcg
      301 cgcagctcgc ctgcatcctg gaaaaggcgc gcgccacgct cgccgccggc ggcgcgctga
      361 tcgatctggc caagcccgac gccttcgccg gctccgtggg cgcgcaagca cgcgcgccgc
      421 tgtcgggcga caagtactgc tggtacgcct cctggctcta cgccacgcgc cgtcagggct
      481 ccggcaccgc gctggttccg tgctacccgg ccgatgaagt gtggctcagc gaggaccgca
      541 ggcaattgtt cctgcgcgtg gcctccggcg aggacgaagt cctgcacgaa gtggcaagcg
      601 gcaccatcaa ctggtacgac gcaccgcagt tcaccaccgc ttccggcccc gagtgcttca
      661 cgttcggcgc ggtgagtcca cagttcatgg agacctggac gcacgtgttc gccgcgttcg
      721 tcgagttcgt caagggcctt ctccacatca tcaactgcgc caccagcccc aaggagtatg
      781 cgtcgaacat tccgttcatc gtctgggacc tgaccaagac ggtgtccgtc gcactgggcg
      841 aggcgccgct gccttcgctc atcgcacaga aggcgggcgc gggtgtcggc gcgagccagg
      901 tgctgttcaa cctgcttccg atcctgatgg tgctcggcgg ctccttcgag ggcatccaca
      961 ccaagaccac cggtggcaac atcttcctgc agtgggtcac gctgctgggc ggcgatgccc
     1021 tcaacgcctt cagcaaccac agcatcgtga gcaccgcaaa cgacgcgctg ctcagcttct
     1081 ggacgctgat caaccagacc ggcccggccg gcccccccga cggagacgac acccgcccgc
     1141 gcaaccgcga gtacggcggc cccctcatcg gcctggtgaa cacgggcgcc aatgcgctga
     1201 tgatgaagta catcatcccg cgcgaggatt acgcccatcc gttcgaggga cagaacgtca
     1261 agaacttcct catctggtgg ctcgcgggct cgacgctggc gggcgttacg ggcgggctgg
     1321 tcggcaccct gctggtatgg acgctgtcgc gcacctacga ctggccgcag ttcggcgagg
     1381 agatcggcct gggtgcgctg cgctccttcc tcggctttgt cgtgacccag tacgccctca
     1441 tggaaggcga caccgacggc ggcaagtaca atcccaagct cgatcccgaa ggcaacccct
     1501 actctcccgc gcgccagccc ttcgccggct atccccccgc tgacacgtcc ccctacaagt
     1561 tgccttacga gcgcggtgtg tcgatgttcg cgggtcaggc caaccaaggc atgttcagcc
     1621 acatgcgctt caactggctg ccgcagatct acgcttacga cttcgcgcac gacttcggcg
     1681 acgagatcct ggcttcgcgc ggcggaacgg tggtcgactt cttcgactgg atcgaggaca
     1741 acactgatcc agacggcgct gcgcagaccg ccgcgttgac cgcctcggag gccgtgatgg
     1801 gagcgagctg gcgcagcagc gccgagagct ggaacttcat cacgattcgc cacgacacca
     1861 acgtcgcgga ccacgacaag gacgtgaacg gaaccgccac gaccacctac gcaacctacg
     1921 gtcacggcaa gaaggggagc gtgcgcgaag tgttcaacac caagggcgtc gcgcccacgg
     1981 cgatcatcgg tcggacggtg gtgcagggcg aggtgatcat gctcgccgac gataccggcg
     2041 tgagcttcca caatcacctg cacatgcacg tccgcggcgg acccgcaccg cccgcctctc
     2101 cgccggcgac gcccacaccg atcgccgatg caagcctgaa ccagtacacg ctgcccttcg
     2161 tgttccgcga ggccaagcac gtcatcgccc gcgacggcgt gctgaggaac ctgacctggt
     2221 acaagtcgga caacgagaag ttctgacgcc catggacctc ggcctgccct ttctgtccga
     2281 cagcagcccc gcccccggct tcagaccggg ctgcaagatc acgcagggcg ccgccgcgag
     2341 cggcggcggc ggtctgctgg atgcggtcgc cagcctgctg agcgcgccgc aggccgatcc
     2401 ctgggccgag aatctgcgca gcgtggagat cgtgcagggc ccggcgccgt tcctcgatac
     2461 ctgccgcttg ctgctttcgc gcacctccac gacgccgcag ctcgcggtgg acgacgagct
     2521 gggggtgcaa ctcgggttcg aggacagcct gaccactgtg ttcacgggca aggtcgccct
     2581 gctcggcgcc acgcgcgacc aggggctgga ggcggtgctg gcctcgcccg cgcacttgct
     2641 ggcgcagtcc cgccagaacg ccagcttcga gcagcgctcc ttgggcgatc tgctgcggtc
     2701 gtgggccggc gaggcgcagg tcacgcccgg cgatctcgat accggcccga gcttcccctt
     2761 tctcgccgtc gacgaccggc gctcggcttg ggagtggatc ggcgtgctcg cgcggttggc
     2821 gggcttgctg gcttgggtcg acggcgaagg caagctgcag tgcaagcaac cggccggcgc
     2881 gccggcgcgc agctatcgct acggccagga tctgttgtgg ctgcgctcca ccgagcgcgt
     2941 cgccaacctc ggcgaggtga ccgtcagcgg cgagggggcc gccggcagtc aaggcgccaa
     3001 ggcgtggagc tggctcgcca agagcgccga ctcgatgcaa gcgagcgccg gtgacggcgc
     3061 gcccaagcgg ctgtaccagg agggcgcgct gcgcgaacac gcggcggtgg acgccgccgc
     3121 caacggcctc tccgaacgtg cgcaggcgct gcgctcgatc gtcgaagtca cggtgcccgg
     3181 cagcccggag gtcggggtag gcgcgctgtt cgagctggcc gattgtccgg acgggcgcgg
     3241 cgacggcact tacgtggcgc tcggcttgcg ccaccgctat gcgaaggcgc gcggctttgt
     3301 caccgagatc agcggagcgg cgacatgaac gatctgtacg agacgctgcg caagctggtc
     3361 cggcaggaac tggccacctt gcgcgtggcg gagctggcga cggtgcagga ggcgtttcca
     3421 gcggacccgg cgaactacga atgcaccgtg gtcctgcgcg acagccagct ggtgctcaag
     3481 aacgtgccgc tcgccacgcc gcgcaagggc ctggccggcg tgccggatgt gggggatctg
     3541 atgctggtgc agttcgtggg cggcgacctc aaccgcccgg tcatcatcgg ctctctgtac
     3601 aacgacgagg accgtccacc ggagaacaag gagcagcagc tcgtgctgca catgccgatc
     3661 gacgccgatc ccgactctgc gctgcgcctg gagctgaacg aggcctcgcc gatgagcctc
     3721 aagctcaaca tcgggccggc gctgaagctc actctgcagg acgacgatcc ggtggtgagc
     3781 ctggacgtgg ggggcaacgc caagctgacg gtggaccgct ccggcgcggt caagctcgag
     3841 agcaacggcg agctcgccat caaggccggc gccaatctga agatcgaggc cggcggcgag
     3901 ctcaagctca agggcgcggt catcaacctg aactaggaaa agtgatggga caaccggcgg
     3961 ccaagcaagg cgatcaagtg atggcgaccg acatccacat cgagatggtg cccacacccg
     4021 ggggcccggt gcccacgccg ttgcccaatc cgttcaccgg catgctggac ggcagcctga
     4081 gcagcgacgt caagatcgag ggcaagccgg ccgcggtggt gggttccacc gccaccaaca
     4141 cgcccgcgca catcttcaag agcggcccgt tccagaagcc tccggcgaac agagccacga
     4201 ttcagctcgg ctcgagcacc gtgctgatca acggcaagcc cgcggcgcgt aacggcgaca
     4261 ccgccatcac ttgcaacgac cccgccgatc tgcccgccgg cacggtggtg gccgtgggca
     4321 cggtgctcat cggttgaggt cgacggcatg cccgtgcgcg cggaaaagac cgagcacctg
     4381 ctgcgcgacc tcgcgatcga ggccgtgcac cgggagctgc gtccggtgta caccgtcctc
     4441 gacccgaaaa ccccggcgcc caacgggcgc ggaagagtgt gggacctggt cccgcgggcg
     4501 ggcaaggaga acctggcaca ggccgtcatc atgcgcctgc tcacgccgcg cggcgagctg
     4561 gccgcgctcg gccatccgga atacggctcg cgcatccacg agctgatcgg acgcgaaaac
     4621 accggcacgc agcgcaacct gctcaagctg catgtgctcg aagcgctgca gttcgagccg
     4681 cgggtcgcga aggtggtcga gctgaacgtg gtcccctccc cgggcacgcg ctccacggtg
     4741 gacgtgctgc tcagggtgca ggtggcgggc gcggccgagc tggtgcagat cggcccgttc
     4801 agtatcgagc tggcggtcta ggcaacatat gtgaggacgg cggccatgag cttccgcaga
     4861 aagacctacc ccgaagtctc ggatcatctg ttgaaccgtc tgctgggcgg cgtgagcgca
     4921 gaggcgcatg cgtacccgcc gccgaagagc gcgcgcgagc ccttcgccca cccgctggag
     4981 aaggcgccgg tgacggcgat cacttccgtg tacgggctgc tcaacggcga gtcgagcgtg
     5041 ttcgcccggg acgtcgacta tgagctgtcc gcggacggcg cgcagatggt ctggaagacc
     5101 ggcggccggc gcccggacgc cggttccgtg gtggagatca actacctgcc gaagcagcgc
     5161 gagtccggac tcaacgacct gtaccccggg tcggtggtgc gcacactgct ggaggcggtg
     5221 gcgctcgaga ccgcggggct gtacgcgcag atggagaccg tctaccgctc gggcttcatc
     5281 gacacggccg acggcggcgc gctcgatcac gtcgtctcgg tgctcggcct gcagcgggta
     5341 aaagccggac gcaacgtggc cgagatcgag ctgacgcgca ccaagaacac gcgcggcgaa
     5401 atcctggtcc cggccggcac gcgcgtgctc accgccgacg gcggcatcga gtacgagacg
     5461 ctggccgaca ttacgctggc cgacgggcag cccggcgcga agggcagcgc acgcgacctg
     5521 gtggagacca acgacgccgt cccggccggc agcctcacgc tgctggccaa acccatcgcc
     5581 gggatcgact cggtgaccaa ccccgccccc agcacgcgcc tggaccgcga cgagaccgac
     5641 gccgagctgc gcacgcgcgc caagagcttt ctcgccggtt cggagcgcgg tacgctgggc
     5701 gcgatcaatg ccgcgattgc gcgccagggc atcctggccg acgtggacga cactgagccc
     5761 ggactgatcc acattctgtt ccacgacgat caacttgcac ccgagcagcg caagcggctg
     5821 gaagacgcgg tgcacgccgt gcgaccggcc ggcgtggcgg tgcagttcga gtacgggcag
     5881 ccgccgctgg cggtggacct cgagatccgc ttgaccagcg cgccgggtct gctcgagacc
     5941 gacttgaagc acatccagca ggacgtgcgt gcacgcgtca ccgattattt cgccaagctg
     6001 ccgaccaagg ccgccggcag cgtgagcaag ctgatcggcc tgaccatggg tgtggagggg
     6061 gtggaggacg tcgccatcgt ctcggccgtc gcgggcggcg acgacgtgct cgacaccgcc
     6121 aagggcgagc tggccatcgc cggcacgccg actcagctcg gcgcgctcaa catcgtcgat
     6181 ccggcgctgg ccactctgct ggtcgcgctc gtgcgctaca cgacggagca aaagatcccg
     6241 gaccaggcgg ccatccagac ggctttgcag accgcggtca gctacttgaa cgagctcaac
     6301 gccgcccccg ccgcccccct ccagaagcgc acgctgtcgt ggggcaagct cacgctcgcc
     6361 acgccgctgc cggatctcac cgccgcgacg ctggccgctt acgatgccaa ccccggtgca
     6421 ttcacgctgc ccactgcggc gcagcgcgcg ccgtacacgt tgcagttcgt gttcacacgt
     6481 cccacgggtg tgagccaggt gctggacagt gaagcggtgc cggcgctggt gctggcgccc
     6541 ttcgagcggc tgtcgctggc caaggtgagc accgaggtca agcccaaagg agccagcgcg
     6601 tgagcgcgca cgaccggctg cgcgatctgc tgccgtcgct ctaccgcccg gagccgggcg
     6661 agggcacgct ggtcaatcgc atgctgctcg cctcgggtat cgtgttcgac ggcgtggcgg
     6721 tccagctgca gcacgtgctg cgcgcgcact ggttcgacat cgccgacaag gcgacctggg
     6781 acgcgcacta tcagaccgcg cggcgcgagc gtggcttgcc ggccgccaac gtgcgcaacg
     6841 tcaaggacct caaggagatc ctgcaatatc cgtacgtcgc ggacctggcg cgcgtgtgtg
     6901 cgctgctcga gcttccgccg tggctcgagc ctacggatct gcgcgagagc gtggaggagt
     6961 accgccagcg cgtgcgcgac gtgctcgacg cctaccgcct gggcctgacc actttggcgg
     7021 cgctgcgtcg gttgaccgaa gccgcgctgc ccgaggacat gggcgcgccg ctgccgcagc
     7081 agcgctggcc gtttgcgatc gaagagccgg tggcactcac gcgcagccgc acccagatcc
     7141 tcgtgcccga cgcgcaagag ggcgacctgg tgagcccctt gttccgctgg acgctgggcg
     7201 acgtgcaggg cacgccgacc gtctacatcc aaggcgtggc gcccagcggt gagctgatcg
     7261 ccgccaccga gcagcccgcc atcgagcgct tcacgccggg cgagacaccg gccgggacgg
     7321 ctctggccta cctcggcacc ttggcgcagg ggcagacgtt gcggctgtcg ccggcgcggc
     7381 gcacctggct ggtgctggcc ggcgcattgc aggcttcacc cgaggagagc gcggccaacg
     7441 ccgcgcagga tccttccgcc aacggtccgt ggacggcgct gtccaatgca ccaaccggac
     7501 aagtgcgatt gctggcgcga gcgttggaca acacgttgtg gctggcgctg gaagacggcg
     7561 ggaactggag cctgcatcgc tacgacggcg ccaagttcga cgccgtcacg cagggcgcgc
     7621 cggcgggacc ttatctctgt ctgcgggcgc aaggcgccgg tgtctatctc ggcgcccagc
     7681 aagggttgtt ccgctgtgcg ctgttccccg acgccaatca gccgttcgcg ttgggcgccg
     7741 tgaccgcagt gcccgaggcg gtgcaggcgc tcgccgtact gcccgacggc cagctcgcct
     7801 gcgccggcgg gaacgggctc gcacggctcg cgcccgacga cacgctggcg gatcgcctgc
     7861 tgcccggtgt gggtctgcgt gcggtgcagg cagaccgcga ccagctgtat ctggcgacgg
     7921 cgcaggcgct gctgctgtgg gatcacgagc gctggttccg ctacgagggc gcgcagctga
     7981 gcgaaggcgc ggccgactgg gtggcgctcg acgcgggcga ggtcgccgct gcggtgagtc
     8041 cgctgccggc ggtgaatgcc atcgcggtca cgcccgacgg cagcgtctgg ctgggcacgg
     8101 cgcgcgggct ggcgcgctat tacgtccacg gcgatcgcac cacgttgctc gaggcgttcc
     8161 ccgatctggg cagcggcgag atccgtgcgc ttacggtgga cgagcgcggt atgctatgga
     8221 tcgccgccga cgacgggctg ttccgttacg acggccgcga cctcgcgcaa tacgacttcg
     8281 ccgagaagcg ctgggtgcag ctggggccag ccgacgccgt ctatcccgac gggatcagcg
     8341 cggcggcgcg cggccattgg cgcttcgacg gcgcgcagga ccgctgggag cgcttcgacg
     8401 cagcggccgc gcgtttcacc actttcccgt tgagcaagcg cgccggcaac agcgcggcgg
     8461 cgcaagcgct gttggtggcc gacagcctgc gcgcagagct gggccagttc gacggcaacg
     8521 agttcagcga ttcgcagccg gtggcggcga gccagctcgc gctgcgcatc aagcccgacg
     8581 agacgcgcat cgtcgctggc gggctgcctg cgctgccggg tggcgcaggc acctggcgct
     8641 atctgcagct ggaaccggcc aatcttgcgc cgccgtcggg acggccctgg tggtcgcgcg
     8701 aaggtcggct gttcttgccg ccggagtggc aggcgccctg gcccggccac ttccgcaacg
     8761 aagccagcgc gtggcacgcc gacggccatt tcgacgacgc cgtgttcgtc tatccgccct
     8821 cggcgcgcgt gtggatggaa tatccggtga gtccggtcgt gggcgtgcgc gtgcggctgt
     8881 tccggcgcat gcccgaccag gcggtcgacc cggcattgat cgatcgcgtg tggcagttgc
     8941 tggcgcgcgc caaggccgcg ggcgttccgc tgcagctcgc cgtggaaggc atggtcgtca
     9001 aaggagagtg agtcatgagc ttgcaggatg ccattaccca gattcaaggc ctggccccca
     9061 aggctgccgg ccatctcgtc cgtccccagg actggaacgc gctgatcgcc gcgctcggcg
     9121 agttcggcgc cgccatcacc accaacaccc aggacctggc cacgctgcaa acgcaggtgc
     9181 agcagatcct gactcagctc ggtcaagtgg tcgggcaggt gcaggtgctg gacagccgcg
     9241 tggacgagct cgagcagagc atccagccgc tgctcggcaa ctacctggtg acgctgtcct
     9301 gcgaccgcct gacctacgcg tccggcgaaa tctgcgagct gaccgccaac gtcaccgatc
     9361 tgaccggcca ggccctggcc gcgcctttcc cctgggtgga cttcgtcgcc gcctggggca
     9421 ggctgcgcgc cgtggccggc ttcaacagtc gcgccggcag cggcgacaac tcggtctcgg
     9481 tgcaggtcaa cgcgcaaggc gtggccaagg tgcagctgcg ggccgagcac agcgagggct
     9541 tctccgagat cgaggaagca caggtcagcg cggtgatgca gatgcaggtg cccggccaga
     9601 acaagagcgt ggcgcaggta ttcatgggcg cgcccacgcc gtccgatgcc ggctccaagt
     9661 cggcctacaa ggtcttgcac gcggagtacg agcggcagga ctcgatcgcg ctgcgcgctt
     9721 acgccgacac ctaccacgtg cgcacgccgg agtggagcct cgcgccgctc ggccccaact
     9781 actggacaca gtggcacgac taccgcgcca cggtgatcgc ccaggcccag ccggacgccg
     9841 cccccaccac gccggacaac gcccgcggca cggccagcat ccaggtcacc ttccgcgact
     9901 ggctgtggcc ctggacgctg gactacctgg aggacctgca cgtgctgcag gaccaggtgt
     9961 tcgagcaata ccagccgctg ttccagttcg cggacgtgta cgacaagttc cagcagaagg
    10021 tcgacggcga tctgcccaag tacggcgtga tcggccgcaa gaagtacctg tccgccgcgc
    10081 agaaggcgat cgagctgatc aatcccggct cggatccgat caagcagaac gccaggcagc
    10141 agatcaagca ggccgtctcc gcgcaagaga gcaacgagat ctacgccggc ggcaaggcgg
    10201 ccgcgggcgc gccggtgatg gggatctacc tgggacaggg caaatcgacc gacgacgtcc
    10261 ggcagcaggt caagcaggtg gaccagaaag tggaggagac cgtgggcgtg caggctgcgg
    10321 tcaacgtgct ggaggggcgc atgcagtcg
//
LOCUS       DASPGE010000175         4635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1451169, whole genome shotgun sequence.
ACCESSION   DASPGE010000175 DASPGE010000000
VERSION     DASPGE010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451169"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VD791_05540"
     CDS             <1..520
                     /locus_tag="VD791_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV07470.1"
                     /translation="AEQLSNCGGAQLAARHKALSADHLEHLDEAGAMALREAGTVAVL
                     LPGAFYFLRETKLPPLDLLRRHGVPVALATDANPGSSPTTSLLLMLSMACTLLRMTVA
                     EALAGVMQHAARALGQSQRHGALAVGRDADFVLWDIDSPAELAYWFGRNPCRCVVRGG
                     RVVHGESVLPSA"
     gene            517..1899
                     /locus_tag="VD791_05545"
     CDS             517..1899
                     /locus_tag="VD791_05545"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HXV07471.1"
                     /translation="MNALFAEHALLPDGWARNVWLAWDSAGVLIKVKAGASSDGELTR
                     AAGPVLPGMPNLHSHAFQRAMAGLTEYQAPGENDFWSWRALMYRFALQVHPEELEAIA
                     RQLYIEMLKCGYTSVCEFHYLHHDPQGRPYANRLELAERLLAAAGAAGIGLTLLPVLY
                     EHSGFGKQPPLSEQRRFVTAPDDLFEMLAALRGAHPEHDRLRYGVAPHSLRAVSPESL
                     ARLVAGVRTVDAVAPIHIHAAEQTAEVADSLAVLGAPPVLWLLDHAGVDGHWCLVHAT
                     HMRQKESARLAASGATVALCPSTEANLGDGIFDAARYLAAGGTWGIGSDSHIGVSVRE
                     ELRGLECAQRLLHRKRNVLAQAGAPRVAEHLYLQAVAGGARASARRISGLAVGQYADL
                     VVLDRNSPDCSLEDPASALSAWIFGRHGNDAVRDVMVGGRWAVKAGHHAGEERALRDY
                     VRARDALLER"
     gene            1912..2724
                     /gene="hutG"
                     /locus_tag="VD791_05550"
     CDS             1912..2724
                     /gene="hutG"
                     /locus_tag="VD791_05550"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HXV07472.1"
                     /translation="MPAHAGNTFELKRGTAPLLISMPHAGTFLPAEIAGRLTDYALRL
                     PDTDWHLERLYEFVDALGASVIVATHSRYTIDLNRPPDGANLYPGQDTTALCPVDTFD
                     RQPLYREGQQPDAAEIQLRIERYWRPYHAALQGELGRLRERHARVVLWDAHSIRSAVP
                     RFFAGRLPDLNFGSAAGRACDPRLAALLLETARQHPRYASVLDGRFKGGHITRHYGRP
                     AQGVHAIQLELAQRAYMREDYPYSFDERLATALRPALIEMLDAALRWVRPGA"
     gene            complement(2812..4329)
                     /locus_tag="VD791_05555"
     CDS             complement(2812..4329)
                     /locus_tag="VD791_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV07473.1"
                     /translation="MGTYLSDRELAQVETAENTFRSPIPTQVISNGEFNPLPQTPQQK
                     QVETRIKELADAYGKKLNMDRRQFLRTSSGMAAAFLAMNGVFGRLFDVSEAEAADVAM
                     AEHRASTLRNQFIFDVQTHFVRDDFQQEGFVGFLKEGAKMWKSGVDPNKVSIYDIKFE
                     NYVRQIYLNSDTSVALLSGAPFDDASWVFLNNQSIAEAVRMVNATAGSTRMLGHAVVT
                     PGQPGWMEEVDRALAERPPASWKLYTIGDPLSAATKYPFRLDDEKLMYPFYAKADKAG
                     IRTMCIHKGLMPADYEQSWKGVWQYQTPQDLLKAAKDWPQLNFIIYHGCLRAFMDPPG
                     SALAEFERTGEIKWASDLARVPQTTGTQNVYAEMGTSFAIAAVVDPRFAAALLGTWIK
                     GLGASNVVWGTDSVFHGSPQWQIEAMRRIEIPEDMRKKHGFAALGPANGVVKSQIFGL
                     TSARLYNLDLRADYQRFTEDKFAQIKHEYRTAGKLDALRDNAAYGYIAKRPTAWV"
BASE COUNT          684 a         1608 c         1568 g          775 t
ORIGIN      
        1 cgccgagcag ctctccaatt gcggcggcgc gcagctcgcg gcccgccaca aggcgctgtc
       61 cgccgatcat ctggagcacc tggatgaagc gggggcgatg gcgctgcgtg aagccgggac
      121 ggtggcggtg ctgctgcccg gcgccttcta cttcctgcgc gagaccaagc tgccgcccct
      181 cgatctgctg cgccgccacg gcgtgcccgt ggcgctggcg accgacgcga accccggcag
      241 ctcgcccacg acctcgctgc tgctgatgct cagcatggcc tgcacgctgc tgcgcatgac
      301 cgtggccgag gcgctcgcag gcgtgatgca gcacgccgcg cgggcgctcg ggcagtcgca
      361 gcggcacggt gcgctcgcgg tcggacggga cgccgacttc gtgctgtggg acatcgactc
      421 gccggccgag ctggcatact ggttcggacg caacccatgc cggtgcgtgg tgcgtggcgg
      481 gcgcgtcgtg catggcgaaa gcgtgctgcc gagcgcatga acgcgctgtt cgcggaacac
      541 gctctgctgc cggatggctg ggcgcgcaac gtctggctcg cgtgggacag cgccggcgtg
      601 ctgatcaagg tgaaagcggg ggcgagctcg gacggggagc tcacccgcgc ggccgggccg
      661 gtgcttcccg gcatgccgaa tctgcactcg catgcgttcc agcgcgccat ggcggggctg
      721 accgagtacc aggcaccggg cgagaacgac ttctggagct ggcgcgcgct gatgtaccgg
      781 ttcgccttgc aggtccatcc cgaggagctg gaagcgatcg cccggcagct ctacatcgag
      841 atgctcaagt gcgggtacac ctcggtgtgc gagttccatt acctgcacca cgatccgcaa
      901 ggccggccct acgccaaccg gctcgagctc gccgaacgcc tcctcgcggc cgccggcgcc
      961 gctggcatcg gcttgacgct gctgccggtc ctgtacgagc acagcggctt cgggaagcaa
     1021 ccgcccctgt ccgagcaacg ccgcttcgtg accgcacccg acgatttgtt cgagatgctg
     1081 gcggcgctgc gcggcgcgca tcccgagcac gatcgattac gatacggggt ggcgccgcac
     1141 tcgctgcgtg cggtctctcc cgaatcgctg gcccgactgg tggccggcgt ccgcacggtc
     1201 gatgccgttg cgccgattca tatccacgcg gcggagcaga ccgccgaggt cgccgacagc
     1261 ctcgccgttc ttggcgcgcc gccggtcctg tggctgctgg accacgccgg ggtcgacggg
     1321 cactggtgcc tggtgcacgc cacgcatatg cgccagaagg agtcggcacg tctggctgcc
     1381 agcggagcga ctgtggctct gtgcccctcg acggaggcca atctcgggga cggcatcttc
     1441 gacgcggcgc gctatcttgc ggctgggggg acgtggggca tcggctcgga cagccacatc
     1501 ggcgtcagcg tgcgcgagga gctgcgcggg ctggagtgcg cgcaacgctt gctccaccgc
     1561 aaacgcaacg tcctggcaca ggctggagcg ccgcgcgtgg ccgagcacct gtacctgcag
     1621 gcagtggcgg gcggcgcgcg ggccagcgcg cgccggatct cgggccttgc ggtcggacag
     1681 tacgccgacc tcgtggtgct ggaccggaac agccccgatt gctcgctgga ggatcccgcg
     1741 tctgcgctct ccgcatggat cttcgggagg cacggcaacg atgcggtccg cgacgtcatg
     1801 gtgggcggcc gctgggcggt gaaggccggc catcatgccg gcgaggagcg cgcgctgcgc
     1861 gactacgttc gcgcgcgcga cgccctgctc gaaaggtagt cctcggccgc gatgcccgcc
     1921 cacgccggca acacgttcga gctgaaacgc gggaccgcac cgctgctgat ctcgatgccg
     1981 cacgcgggca cgttcctgcc agccgagatc gccggtcgcc tcacggatta cgccctacgc
     2041 ctgccggata cggactggca tctcgagcgc ctgtacgagt tcgtcgacgc gctcggcgcg
     2101 tcggtgatcg tcgcgacgca ctcgcgctac acgatcgatc tcaaccgtcc gcccgacggc
     2161 gccaatctct accccggcca ggacacgacc gcattgtgcc cggtggacac gttcgaccgc
     2221 cagccgctgt accgggaagg gcagcaaccg gacgccgccg aaatccagct gcgcatcgaa
     2281 cgctactggc ggccttacca cgcggctctg cagggcgagc tcggtcggct gcgcgagcgc
     2341 cacgccaggg tcgtgctgtg ggatgcccat tcgatccgct cggcagtgcc gcgtttcttc
     2401 gcgggtcggt tgccggatct gaacttcggc agcgcggccg gaagggcatg cgacccgcgc
     2461 ctcgccgcgc tgctgctgga gaccgcgcgg cagcatcccc gttacgcatc ggtcctcgac
     2521 ggtcgattca agggcgggca catcacgcgc cactacgggc ggcccgcgca aggcgtgcac
     2581 gcgatccagc tcgagctggc ccagcgcgcg tacatgcgcg aagactaccc gtattccttc
     2641 gacgagcgtc tggctactgc gcttaggccc gcgctgatcg agatgctgga tgcggcgctg
     2701 cgctgggtga gacccggcgc gtagagagaa gcgccgcggg agagaaccgg tccctcccgc
     2761 gactgctaac cggctcgccg ctgaaatgcg cgcggcgagc cggctgaact gctataccca
     2821 agccgtcggc cgcttggcga tatacccgta ggcggcgttg tcgcgcagcg catcgagctt
     2881 gccggccgtg cggtactcgt gcttgatctg cgcgaacttg tcctcggtga accgctgata
     2941 gtcggcgcgc aggtcgaggt tgtagaggcg cgccgaggtg agcccgaaga tctggctctt
     3001 caccacgccg ttcgccgggc ccagcgcggc gaagccgtgc ttcttgcgca tgtcctccgg
     3061 gatctcgatc cggcgcatgg cttcgatctg ccattgcggc gagccgtgga acacggagtc
     3121 ggtgccccac acgacgttgc tggcgcccag gcccttgatc caggtaccga gcagcgcggc
     3181 cgcgaagcgc gggtcgacca ccgccgcgat ggcgaagctg gtgcccatct ccgcgtacac
     3241 gttctgggtg ccggtggtct gcggcacgcg cgccaggtcg ctggcccact tgatctcccc
     3301 ggtgcgctcg aactcggcca gagcgcttcc cggcgggtcc atgaacgcgc gcagacagcc
     3361 gtggtagatg atgaagttca gctgcggcca gtccttcgcg gccttgagca ggtcctgtgg
     3421 tgtctggtac tgccacacgc ccttccagct ctgctcgtag tccgccggca tcagcccctt
     3481 gtgaatgcac atcgtgcgaa tgcccgcctt gtcggccttg gcgtagaacg gatacatcag
     3541 cttctcgtcg tcgagccgga acggatactt ggtcgcagcc gacagcgggt cgccgatggt
     3601 gtacagcttc cagctggctg gcgggcgctc ggcgagcgcg cggtccacct cctccatcca
     3661 cccgggctgc cccggggtga cgacggcatg accgagcatg cgcgtgctgc cggccgtcgc
     3721 attgaccatg cgcaccgcct cggcaatcga ctggttgttg aggaacaccc atgaggcgtc
     3781 gtcgaagggc gctccgctca acagggccac cgaggtgtcg ctgttgaggt agatctgccg
     3841 cacgtagttc tcgaacttga tgtcgtagat gctgaccttg ttcggatcga cgccgctctt
     3901 ccacatcttg gcgccttcct tgaggaagcc gacgaagccc tcctgctgga agtcgtcgcg
     3961 cacgaagtgc gtctggacat cgaagatgaa ctggttgcgc aacgtcgagg cgcggtgctc
     4021 ggccatcgcc acgtcggcgg cttccgcctc ggagacgtcg aacagcctcc cgaacacgcc
     4081 gttcatggcc aggaaggccg cggccatgcc cgagctggtg cgcaggaact ggcgccgatc
     4141 catgttcagt ttcttgccgt aggcatcggc cagctccttg atgcgcgttt ccacctgctt
     4201 ctgctgcggc gtctgcggca gcgggttgaa ctccccgttg gaaatcacct gcgttggaat
     4261 cggcgagcgg aatgtgttct ccgccgtttc gacttgtgcg agctcccggt cgcttagata
     4321 ggtgcccatg ccctcactcc cccgtgtgcg cgccccaacc ccgcctgctc gacccgcgaa
     4381 cggtcccccg gcaacaggcc ccggggcgcc caatggggcc tgcgccttcc cttgccaggt
     4441 ctcgacggca tcatactgga actgctgccg cgcaccgtca tcgaaccgca tctgcatcgc
     4501 ccaagaaacc gcgagggctg tagggtcgcg gcgctgcgcg aacatctgcg cacccgttcc
     4561 cggtcgagca caatagcggt ccatccatcc ggagccacgc catgcccctg accatgtacc
     4621 atgcttccgt cccgg
//
LOCUS       DASPGE010000176         5905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_866871, whole genome shotgun sequence.
ACCESSION   DASPGE010000176 DASPGE010000000
VERSION     DASPGE010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866871"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VD791_05560"
     CDS             complement(<1..558)
                     /locus_tag="VD791_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HXV07474.1"
                     /translation="MNKAVTTIRTTYREAVRMALREALQRDPRVFLMGEDVGRYGGTY
                     ACSKGLLEEFGEDRVRDTPLSESAFVGAGIGAALGGMRPIVEVMTVNFSLLALDQIVN
                     TAATLRHMSGGQFSVPLVIRMATGGGRQLAAQHSHSLEGWYAHVPGLRVVAPATLEDA
                     RGMLWTALQDPDPVIVFEHALLYNLE"
     gene            complement(662..1582)
                     /gene="pdhA"
                     /locus_tag="VD791_05565"
     CDS             complement(662..1582)
                     /gene="pdhA"
                     /locus_tag="VD791_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HXV07475.1"
                     /translation="MTLERDTAVALLRQMIRIRRFEEKCAEAYSAGKIRGFLHLYIGE
                     EAIAPGVMPALTREDAVVATYREHGHALARGLAAQSVMAEMYGKQEGCSRGRGGSMHL
                     FDKETRFYGGNAIVGGGLPLAVGLALADKLSGRNLVTACFFGEGAVAEGEFHESMNLA
                     ELWKLPVLFLCENNLYAMGTALARSESDTDLVTKAASYNMAAARVDGMDVEAVHEAAH
                     EAVATIRRLGVPYFLELQTYRFRAHSMFDPELYRSKAEVEKWKKHDPIDTFSTRLRAA
                     TVVTDQQIAGIEADVAQEIARAVAFAEAGT"
     gene            complement(1584..3347)
                     /gene="acsA"
                     /locus_tag="VD791_05570"
     CDS             complement(1584..3347)
                     /gene="acsA"
                     /locus_tag="VD791_05570"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HXV07476.1"
                     /translation="MERGLIEKPERTDLPPPNLQRYDEACRQFSWDAARAELAGLPGG
                     ALNIAHEAVGRHATGPRARQLAIRWLGRDGQVQDYTYAELDRLSARFANVLAQLGLNP
                     CERVFILAGRIPQLYVALLGGLKRGCVISPLFSAFGPEPIQTRLAIGEGAVLVTTSSL
                     YARKVAPIRSNLSSLRHVLVVRDALEPDLPPGTLDLQALLDGASDRFDTVPMRPEDPA
                     LLHFTSGTTGKPKGAVHVHEAVVAHRVTGRLALDFHDGDVFWCTADPGWVTGTSYGIV
                     SPLANGVSMIVDEADFDAERWYDILAQQNVSIWYTAPTAVRMMMKGGAEAARRRRFPR
                     LRFIASVGEPLNPQAVLWGEEAFGLPIHDNWWQTETGGIMIANYAAMPIKPGSMGKPL
                     PGIEAAIVQRLPDGGVQTVDTPGVEGELALKRGWPSMFRAYLNEDERYRKCFAGEWYL
                     TGDLAKRDADGYFWFVGRADDVIKSSGHLIGPFEVESAFMEHPAVAEAGVIGKPDPIA
                     GEIVKAFVALKPGREPGPALERELLGFVRKRLGAAVAPKELTFLPALPRTRSGKIMRR
                     LLKARELGLPEGDTSTLESGG"
     gene            3810..3959
                     /locus_tag="VD791_05575"
     CDS             3810..3959
                     /locus_tag="VD791_05575"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HXV07477.1"
                     /translation="MTNISPEQWRMMVAEAAYYRAQRRDFRGGSAEQDWLEAEEEIRR
                     SLEER"
     gene            3983..4402
                     /locus_tag="VD791_05580"
     CDS             3983..4402
                     /locus_tag="VD791_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXV07478.1"
                     /translation="MADITRYDPFRELDDMMRGMVFRPVRMPEPRIEQIKVDATEDDK
                     AYRISAEIPGVRKEDIKVSVVDNEVTISAEVKSQSEKKEGERVVHSERYYGAVARSFT
                     LPQAVDEEGAQAKYENGVLTLTLPKRGNTRMRKIAVT"
     gene            4590..>5905
                     /locus_tag="VD791_05585"
     CDS             4590..>5905
                     /locus_tag="VD791_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXV07479.1"
                     /translation="MSRKRPEDLRSHRWYGAKDLRSFGHRSRTLQMGYSREDYAGKPV
                     IALINTWSEINQCHTHFRQRAEEVKRGVWQAGGFPVEMPAISLSEPFQKPTTMLYRNL
                     LAMETEELLRSYPADGCVLMGGCDKTTPALIMGALSMNLPAVFLPAGPMLRGNWRGAT
                     LGSGSDSWKYWAELRAGNIDEQAWDEIEEGIARSAGTCMTMGTAATMMSLTETLGLAL
                     PGSSSIPAVDSNHSRMAEACGRRIVDMVWEDLKPRDILTSAAFDNAIAVLMALGGSTN
                     AIVHLIAMAGRAGIRLDLARFDEISRRVPLLANLRPSGKYLMEDFYYAGGLRGLMAEM
                     KSLLDLSQRCVNGATLGENIDGAKSYNHDVIATLERPVQSSGGLAVLRGNLAPDGAVI
                     KPSAADPKLLQHTGKAVVFEDYDDMAKRIDDEALAVEASSVLVLRNA"
BASE COUNT          966 a         2040 c         1988 g          911 t
ORIGIN      
        1 ctcgaggttg tagagcagcg cgtgctcgaa cacgatcacc ggatccgggt cctgcagcgc
       61 cgtccacagc atcccgcgcg catcctcgag cgtggccggc gccacgacgc gcagccccgg
      121 cacgtgcgcg taccagccct ccaggctgtg cgagtgctgc gccgcgagct gacgcccgcc
      181 gccggtggcc atgcggatca ccagcggcac gctgaactgg ccgccggaca tgtgacgcaa
      241 agtcgccgcg gtgttcacga tctggtccag cgccagcaag ctgaagttga ccgtcatcac
      301 ctcgacgatc gggcgcatgc cgcccagcgc cgcgccgatg ccagccccga cgaaggccga
      361 ctcggacagc ggcgtgtcgc gcacgcggtc ctcgccgaac tcttcgagca gccccttgga
      421 gcaagcgtag gtgccgccgt agcggccgac gtcctcgccc atcaggaaca cgcgcggatc
      481 gcgttgcagc gcttcgcgca aggccatgcg cacggcttcg cgataggtgg tgcggatggt
      541 ggtcacggct ttgttcatgg cagcgctaga acagccgctc gtcctcggcc ggcggggccg
      601 cggctgtcac ggaagccgcg cgcggcgtgt acacgtcgcg ctcgaggttc tccatcggct
      661 cctaggtgcc ggcttcggcg aaggcgacgg cgcgggcgat ctcctgcgcc acgtcggcct
      721 cgatgcccgc gatctgctgg tcggtgacca cagtcgccgc gcgcagccgc gtcgagaagg
      781 tgtcgatcgg atcgtgcttc ttccacttct cgacctctgc cttgctgcga tacagctccg
      841 gatcgaacat ggagtgcgcg cggaagcggt aggtctgcag ctccaggaag tagggcacgc
      901 ccaggcggcg gatggtggcc accgcttcgt gcgcggcttc gtgcactgcc tcgacgtcca
      961 tgccgtccac ccttgcagcc gccatgttgt agctcgccgc cttggtcacc agatcggtgt
     1021 ccgactccga gcgcgccagc gcggtgccca tggcgtacag gttgttctcg cacaggaaca
     1081 gcaccggcag cttccacagc tcggccaggt tcatcgactc gtggaactcg ccttcggcca
     1141 ccgcgccttc gccgaagaag caggcggtga ccaggttgcg gccgctgagc ttgtccgcga
     1201 gcgcgagccc cacggccagc ggcaggccgc cgcccacgat ggcgttgccg ccgtaaaagc
     1261 gggtttcctt atcaaatagg tgcatcgaac caccccggcc ccggctgcag ccctcctgct
     1321 tgccgtacat ctcggccatc acgctctgcg ccgcgagccc gcgcgccagc gcatggccat
     1381 gctcgcggta ggtcgcaact accgcgtcct cgcgcgtgag ggcgggcatg acgccgggcg
     1441 cgatcgcctc ctcgccgatg tacaggtgca ggaaaccccg gatcttccct gcgctgtacg
     1501 cttccgcgca cttctcctcg aagcggcgaa tgcggatcat ctggcgcagc agcgccacgg
     1561 cggtatcgcg ctcgagggtc atatcagccg ccgctctcca gcgttgaggt atcgccttcc
     1621 ggcaagccca gctcgcgcgc cttgagcagc cgccgcatga tcttgccgct gcgcgtcctg
     1681 ggcagcgccg gcaggaaggt cagctccttg ggcgcgaccg cggcgccgag ccttttgcgc
     1741 acgaaaccga gcagctcgcg ctccagcgcc ggccccggct cacgcccggg cttgagcgcg
     1801 acgaacgcct tcacgatctc gcccgcgatc gggtcgggtt tgccgatgac gccggcctcg
     1861 gccaccgcgg gatgctccat gaacgcgctc tccacctcga acggcccgat caggtgaccc
     1921 gaagacttga tcacgtcgtc ggcccgtccc acgaaccaga aatagccgtc ggcgtcgcgc
     1981 ttggccaggt ccccggtcag ataccactcg ccggcgaagc acttgcggta gcgttcgtcc
     2041 tcgttcaggt aagcgcggaa catcgacggc cagccgcgct tgagcgccag ctcgccctcc
     2101 acgccgggcg tgtcgaccgt ctgcacgccg ccgtccggca ggcgctgcac gatcgccgct
     2161 tcgatgcccg gcagcggctt gcccatggag ccgggtttga tcggcatcgc cgcgtagttg
     2221 gcgatcatga tcccgccggt ctcggtctgc caccagttgt cgtggatcgg caacccgaac
     2281 gcttcctcgc cccagagcac cgcctgcgga ttgagcggct cgcccacgct ggcgatgaag
     2341 cgcaggcgcg gaaagcggcg ccggcgcgcg gcctccgcgc cgcctttcat catcatgcgc
     2401 acggcggtgg gcgccgtgta ccagatcgag acgttctgct gcgccaggat gtcgtaccag
     2461 cgctcggcgt cgaagtcggc ctcgtcgacg atcatgctca cgccgttggc gagcggcgag
     2521 acgatgccgt aggaggtgcc ggtcacccag cccgggtcgg ccgtgcacca gaacacgtcg
     2581 ccgtcgtgga aatcgagcgc gagccggccg gtgacacggt gcgccaccac cgcctcgtgc
     2641 acgtgcacgg cgcccttggg cttgccggtg gtgccgctgg tgaagtgcag caacgccggg
     2701 tcctccgggc gcatcggcac ggtgtcgaac cgatcggagg cgccgtcgag cagcgcctgc
     2761 aggtcgagcg tgccgggcgg gaggtccggc tcgagcgcgt cgcgcacgac cagcacgtgc
     2821 cgtaggctgg aaaggttgga acggatcggc gccaccttcc gcgcgtacag gctggaggtc
     2881 gtcaccagca ccgcgccctc accgatggcg agccgggtct gaatcggctc gggcccgaag
     2941 gccgagaaca gcggggagat cacgcagccg cgcttgagcc cgccgaggag cgcgacgtac
     3001 agctgcggaa tgcgcccggc caggatgaac acgcgctcgc acgggttcag gcccagctgg
     3061 gcgagcacgt tggcgaaccg tgcggagagc cggtcgagct cggcgtaggt gtagtcctgc
     3121 acctggccgt cccgtcccag ccagcggatc gcaagttgcc ttgcgcgcgg cccggtggcg
     3181 tggcgcccga ccgcctcgtg cgcgatgttc agcgccccac ccggcagccc ggcgagctcg
     3241 gcgcgcgctg cgtcccagct gaactggcgg caggcctcgt cgtagcgttg caggttgggc
     3301 ggcggaaggt ccgtccgctc aggcttctcg atcagccccc gttccatcgg ctcctcatcg
     3361 cgggacggcg caacggcgtc gcgccggacc gagtaaacgc taacaactcc ccgcgcccga
     3421 gcccttgatc gaggtcaagc gccttcacaa gcgcttcacg ttaccgggcg atgattgccg
     3481 tcgcggccgc ctgcagcatt gcccgccggg cacctgcgcc cttgatacag gtcaaaagcg
     3541 gcgagagtgg gtcgagggat aattctgcca cggagcgagc ggcgctctcg cgttcgcctg
     3601 gctgcaaacg aggaggttat tcgcgcatga gcggtatcca gagcccgcgt ccgggcacac
     3661 cgggcgcccc ccagacacgc aagagcaggg aaggttcgca ggcatttggg cctgcgtcga
     3721 aaagtcgaac gagccccggg cggtcgagcg gccacccccg cgcaccggcg gagccggatt
     3781 ccaagccaat cagtgaagcg ctgagcccga tgacgaatat ctcgcccgag cagtggcgca
     3841 tgatggtggc cgaggccgcc tattatcgcg cgcagcggcg cgatttccgt ggcggctcgg
     3901 ccgagcagga ctggctcgag gcggaggaag agattcgtcg cagcctcgag gaacgctgaa
     3961 tcgctttgag aggagcagca acatggccga catcacacgc tacgacccat ttcgagagct
     4021 ggacgacatg atgcgcggga tggtgttccg tccggtgcgc atgcccgagc cgcgcatcga
     4081 gcagatcaag gtggatgcca ccgaggacga caaggcctac cgcatcagcg ccgagatccc
     4141 cggggtgcgc aaggaagaca tcaaagtgtc cgtggtggac aacgaggtga cgatttccgc
     4201 cgaggtcaag agccagagcg agaagaagga aggcgagcgc gtggtccaca gcgagcgcta
     4261 ctacggcgcg gtggcgcgct ccttcacgct gccccaggcg gtggacgaag aaggcgcgca
     4321 agcgaagtac gagaacggcg tgctgaccct gacgctgccc aagcgcggca acacccgcat
     4381 gcgcaagatc gccgtgacct gacctccgcc tacccagggc tttcttctcc agtccgcgca
     4441 ataacaccgc aagcagaacc cgtgttgcgg tgagcgtgcc cggccggtgc agcgatgcgc
     4501 cgccgggctt ttttttggcg atcggcgccc gatgatatgg tatcgtgcgc cgctttccct
     4561 cgccgtattc gccctcccag agcacgttca tgtcacgcaa acgccccgaa gacctccgca
     4621 gccaccgctg gtacggcgcc aaggacctgc gctccttcgg ccatcgctcg cgcacgctgc
     4681 agatgggcta ctcgcgcgag gattacgccg gcaagccggt gatcgcgctg atcaacacct
     4741 ggagcgagat caaccagtgc cacacgcact tccgccagcg cgcggaggag gtcaagcgcg
     4801 gcgtgtggca ggcgggcggc tttccggtgg aaatgccggc gatctcgctc tccgagccgt
     4861 tccagaagcc gaccaccatg ctgtaccgca atctgctggc gatggagacc gaggagctgc
     4921 tgcgctccta ccccgccgac ggttgcgtgc tgatgggcgg ctgcgacaag accacgccgg
     4981 cgctgatcat gggcgcgctg tccatgaacc tgccggcggt gttcctgccc gccggcccga
     5041 tgttgcgcgg caactggcgc ggcgcgacgc tgggcagcgg cagcgattcc tggaagtact
     5101 gggccgagct gcgcgcgggc aacatcgacg agcaggcctg ggacgagatc gaagaaggca
     5161 tcgcgcgctc ggccggcacc tgcatgacca tgggcacggc ggccaccatg atgagcctga
     5221 cggagacgct ggggctggcg ttgcctgggt cgtcttcgat tcccgcggtg gattcgaacc
     5281 actcgcgcat ggcggaagcc tgcggccggc gcatcgtcga catggtatgg gaggacttga
     5341 agccgcgcga catcctcacc tctgccgcct tcgacaacgc catcgccgtg ctgatggcgc
     5401 tcggcggatc gaccaacgcc atcgtgcatc tgatcgccat ggccggccgc gccggcatca
     5461 ggctcgacct ggcccgcttc gacgagatct cgcgccgcgt gccgttgctc gccaatctgc
     5521 gcccctcggg caagtacctg atggaggact tctattacgc gggcgggctg cgcgggctga
     5581 tggccgagat gaagagcttg ctcgacctgt ctcagcgctg cgtcaacggc gccactctgg
     5641 gcgagaatat cgacggtgca aagagctaca atcacgatgt gatcgccacg ctggagcggc
     5701 cggtgcagtc gagcggcggg ctggcggtgc tgcgcggcaa cctggcaccc gacggcgctg
     5761 tgatcaaacc cagcgccgcc gatccgaagc tgctccaaca cacgggcaag gcggtggtgt
     5821 tcgaggacta cgacgacatg gcaaagcgca tcgacgacga agccctggcg gtggaggcct
     5881 cgtccgtgct ggtgctgcgc aacgc
//
LOCUS       DASPGE010000177         7135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1687107, whole genome shotgun sequence.
ACCESSION   DASPGE010000177 DASPGE010000000
VERSION     DASPGE010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687107"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VD791_05590"
     CDS             <1..531
                     /locus_tag="VD791_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07480.1"
                     /translation="AEHLSGAAQGDAGLGARAALIVQRLPRLPRSLRLLLQRNPLARL
                     LFQHAGRECLHDRAVAGALLQAGEPHVRALAVEALTADQPAAHEALRHHLDAVLAALD
                     SPLSAPVKRRAMRALARAPRDPDGAQRTVAWARRALERGADAGALELVGRLIAQHPSL
                     QDGGEAPVVYRRATSH"
     gene            1195..2418
                     /locus_tag="VD791_05595"
     CDS             1195..2418
                     /locus_tag="VD791_05595"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HXV07481.1"
                     /translation="MPIPAAAPIAQPPAPEPPARKPSQRRLTLRDPRVPVPAHPKRRR
                     RGRARLLPSEESRRLFADTLRSADASLRHLATDEAQLARYGMPVWRSEVELAQALGLT
                     VKALRHYAIHSARERAPHYVMFAVPKRSGGERLIMAPKSRLKAIQRRLNALLVGKLPA
                     SEYAHGFVARRSIRSNAQPHVGKAVVLHLDLKDFFPSIHFGRVRGLMIALGYGYPVAA
                     ALAALMTESPRHPVMAGGVRYFPPVGARACPQGAPTSPGLSNAIVAKMDRRLAGLARR
                     MGFAYTRYADDLAFSGDGVREAHALRCLAQRIIEDEGFALNHAKTRVLRSGTPQRITG
                     VIVNDVLGLSRQERRRLRAALHQASLRARSEGARLQIDPVLRGKLAYLNMLNPAQAAR
                     LVAAVRSTLTRHSGG"
     gene            2422..4953
                     /locus_tag="VD791_05600"
     CDS             2422..4953
                     /locus_tag="VD791_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HXV07482.1"
                     /translation="MIPNAPRHPLVLLALGLVLLPWLTLAFGFTWGIATEIAIFAMVG
                     IGYNVLLGYTGLLSFGHGLFFGLAAYCVALTQIHWFPDSGVLPFASGLLLATLLGAIV
                     GFLALRRRGVYFSLLTLAFTALTFYIVFRWTSFTGGENGLSGIRRQPLLGVPLDDARL
                     FYYVTAAVVLAVAWLMWRVVRSPLGTVLVAIRENEQRARFAGYPVQHYKLAAFTISAA
                     VVGLGGCLAAYLKLFVSADLVHVNFSGEILAMTIFGGMGSFLGPALGGFLYLMFREVV
                     SGHTAAWQFWFGLLFMALILFSPLGFVGLSERLTAPLRRKRVDAAAMAARVTPQPGQE
                     APAFLREHPRVDGVLLECRGVTKRFGGFTAVDAVDLRVHDRTLHALIGPNGAGKTTLF
                     NLVSGMFAPDRGKVSLAGRPIQGRTPDAVAARGLSRSFQITSLFPSLSVREHLRLSVQ
                     ARSERRYHPFRPADSLEQVNRETGELVRFLGLEGLEEVPVSSLSYGGQRLVEIGVALA
                     AKPRALLLDEPLAGLAAAERSRITALIRDLTRHMAVLLVEHDIDRVFAVADKITVMNE
                     GKVLVEGDAETVRNHPEVQRVYIGAGHAQVAALERPQPAGPGVPILQVQGINTFYGKS
                     HILHDVSFEVRSGEVVALLGRNGAGKSSTFKSIIGIVPPRSGGVTLTGREVAGMTPER
                     IARLGVGLVPQGRRLFSGLTVDENLRLGALARAGGSGVRWDRSRIYGYFPRVRDKLHT
                     RADQLSGGEQQMVAIARALVGNVRILLLDEPFEGLSPAMVEEVFRSIEQLRKEVSILI
                     IEHHLDLVLSLADRAVVLDRGRVSHAGPAAPLRTDLDFRRQVLWL"
     gene            4976..5749
                     /locus_tag="VD791_05605"
     CDS             4976..5749
                     /locus_tag="VD791_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV07483.1"
                     /translation="MELSMKNQRVLITAAAGGIGRVMAQTFVEAGAKVHICDVAQSAL
                     DETVKALKVTGSRCDVSDRKQVDLLFADARRDLGGLDVLVNNAGIAGPTGKVEEIAVE
                     DWERCIAVDLNGMFYCTRLAVPMLKAAGGGSIINLSSAAGRLGFPFRTPYAAAKWGVV
                     GFTKSLSMELGTDNIRVNAIQPGVVEGERIERVIDAKAKALGIGFEEQKRRSLDKVSM
                     RTMVSPQDIANMALYLASAAGRHVTGQAISVCAGVEMLG"
     gene            5785..6720
                     /locus_tag="VD791_05610"
     CDS             5785..6720
                     /locus_tag="VD791_05610"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HXV07484.1"
                     /translation="MEKIGIVGAGLIGRAWAIVFARAGHPVLMYDADPKAVKAAQPLI
                     REALQELREAGLVSEAAEVVARRIAPVATLRDCVQDADYVQENTAERLEVKREVYAQM
                     DAAAKPDCILASSTSTIQTSRFADGLPGRQRCLVAHPVNPPHVVPVVEISPAPFTAPA
                     VVERTRALHAKVGQVPITVKREVEGFILNRLQAALLNEAWRLIKEDCCTVEDLDKSIK
                     DGLGLRWAFMGPFETIDLNAPGGIPDYAARYGSKLHGMMQDIRYAPWDAALVAKVEAA
                     RRAIMPQAQHAAREAWRDRRLMALIAHKRAMAAKD"
     gene            6740..>7135
                     /locus_tag="VD791_05615"
     CDS             6740..>7135
                     /locus_tag="VD791_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HXV07485.1"
                     /translation="MAEQRKVIITCAVTGAIHTPTMSPHLPVTPEEIAQGAIGAAKAG
                     AAIVHLHAREPKDGRPTQDPEVFKRFLPAIKAASDVVVNLTTGGAPTMSVEERLQPAL
                     KLKPEVASLIMGSMNFGLYEMIPRYKEWKH"
BASE COUNT         1002 a         2513 c         2577 g         1043 t
ORIGIN      
        1 gccgagcacc tgagcggcgc cgcgcagggc gacgccggcc tcggcgcgcg ggcggcgctg
       61 atcgtgcagc gcttgccgcg attgccgcgc agcctgcggc tgctgctgca gcgaaacccg
      121 ctggcgcgcc tgctgttcca gcacgcgggc cgcgagtgcc tgcacgaccg cgcggtggcg
      181 ggcgcgctgc tgcaggccgg cgagccgcac gtgcgcgcgc tcgcggtgga ggcactgacc
      241 gccgaccagc cggcggcgca cgaggcgttg cggcatcatc tcgacgccgt gctcgccgcg
      301 ctggacagcc cgctctccgc gccggtgaag cggcgcgcga tgcgtgcgct ggcgcgcgcg
      361 ccgcgcgacc cggacggggc gcagcgcact gtcgcctggg cgcgtcgcgc gctcgagcgc
      421 ggggccgatg ccggcgcgct ggagctggtg ggcaggctga tcgcgcagca cccctccctg
      481 caagacggcg gcgaggcgcc cgtggtgtac cggcgcgcga cttcccattg acaacgatgc
      541 gcgctgcata cataaagagc gtcgagaagg cgcgctggac cgcaaccagc cggcagtgcc
      601 ccggtgcttc gccggggcgg cgcgttgagc gcatcacttc gccacggttc cccacgccgc
      661 agtcgcgggg aagccatgag tccagagcct cgcaagtggt cggccggcgc gcctcctcgc
      721 ctccattcgt tgcaaccggc catggggctg ttcgctgagc tgctgagctg gctgctcggc
      781 cagcgcaagg cggagccgcg gtcaccggct cgcgggcagc agcggcccgc gcgtcccagc
      841 ggcgcttccc ctcacccccg gcccctctcc cgcagcgggg agaggggagt ttctggtgcg
      901 ccgcgcgcga gcacgccgct cggcgcaacc gttccgcgcg caccagcggg cgtgccgcga
      961 ccgagcacgc cagcgggcgc ggtgcgtgag agcccaccat cgagcggaac ggcgagcgcc
     1021 cgcgcaccgg gcagagcgca caccgcgccg ccgtcccagg cccagccgcc gcaggcggtc
     1081 cagtccgccg cgggcggcgc tccgcgcccc gagccgcccg gtataccagg gaccttgggc
     1141 tcaccaacgg cgacgttggg cgcacccgcg gcgccggcgg ccgcgccgac accgatgccg
     1201 ataccggcgg ccgcgccgat agcgcaaccg ccggcgcccg agccgcccgc gcgcaagccg
     1261 tcgcagcgcc gcctcaccct gcgcgatccg cgcgtgcccg tgccggcgca cccgaagcgg
     1321 cgccgccgcg ggcgcgcgcg cttgctgccg agcgaggagt cgcgccgcct gttcgcggac
     1381 accttgcgca gcgccgatgc gagcctgcgc catctggcca ccgacgaagc gcagctcgcg
     1441 cgctacggca tgccggtctg gcgcagcgaa gtcgagctgg cgcaggccct cgggctcacg
     1501 gtgaaggcgc tgcggcatta cgcgatccac agcgcgcgcg aacgcgcgcc gcattacgtg
     1561 atgttcgccg tgcccaagcg cagcggcggc gagcgcctga tcatggcgcc caagtcgcgg
     1621 ctcaaggcga tccagcggcg cctcaacgcg ctgctggtag gcaagctgcc ggccagcgag
     1681 tacgcgcacg gcttcgtcgc gcgccgctcg atccgcagca acgcgcaacc gcacgtgggc
     1741 aaggccgtcg tcctgcatct cgacctgaag gatttcttcc cttccatcca tttcggccgc
     1801 gtgcgcgggc tgatgatcgc gctcggttac ggctatccgg tcgccgccgc gctggcggcg
     1861 ctgatgacgg agtcgccgcg ccacccggtg atggcgggcg gcgtgcgcta cttcccgccg
     1921 gtgggcgcgc gcgcctgccc gcagggtgcg ccgaccagcc ccggcctgag caacgcgatc
     1981 gtggcgaaga tggatcgccg gctggccgga ctcgcccggc gcatgggttt cgcctacacc
     2041 cgctacgccg acgatctggc tttctccggc gacggcgtgc gcgaggcgca tgcgctgcgc
     2101 tgcctcgcgc agcgcatcat cgaggacgag ggcttcgccc tgaaccacgc caagacgcgc
     2161 gtgctgcgca gcgggacgcc gcagcggatc accggcgtga tcgtcaacga cgtgctggga
     2221 ctgtcgcggc aggagcggcg gcggctgcgc gccgctctgc accaggccag cctgcgcgcg
     2281 cgcagcgagg gcgcgcggct gcagatcgac cccgtgctgc gcggcaagct cgcgtacttg
     2341 aacatgctca accccgcgca ggcggcgcgc ctggtcgcgg ccgtgcgatc gaccctgacg
     2401 agacactcgg gcggctgacc gatgatcccg aatgcgccgc gccatccgct ggtcctgctc
     2461 gcgctgggcc tggtgctgct gccctggctc acgctggcgt tcggcttcac ctggggcatc
     2521 gccaccgaga tcgcgatctt cgcgatggtc ggcatcggct acaacgtcct gctcggctat
     2581 accggcctgc tctcgttcgg gcacgggctg ttcttcggtc tggccgccta ctgcgtggcg
     2641 ctcacgcaga tccactggtt tccggacagc ggcgtgctgc cgttcgcctc cggcctgctg
     2701 ctggccacgc tgctcggcgc gatcgtcggc ttcctggcgc tgcgccggcg cggcgtgtac
     2761 ttctccttgc tcactctcgc cttcaccgcg ctcaccttct acatcgtgtt ccgctggacc
     2821 tccttcaccg gcggcgagaa cggcctgtcc ggcatccggc gccagcccct gctcggcgtg
     2881 ccgctcgacg acgcgcggct gttctattac gtcaccgccg cggtggtgct ggcggtggcc
     2941 tggctgatgt ggcgcgtggt gcgctcgccc ctgggcacgg tgctggtcgc gatccgcgag
     3001 aacgagcagc gcgcgcgctt cgccggctac ccggtgcagc actacaagct ggccgcgttc
     3061 accatctccg ccgccgtggt cgggctgggc ggctgcctcg ccgcttacct gaagctgttc
     3121 gtgtcggccg atctggtgca cgtcaatttc tcgggcgaga tcctggcgat gaccatcttc
     3181 ggcggcatgg gcagcttcct cggcccggcg ctgggcggtt tcctgtacct gatgttccgc
     3241 gaggtggtct cgggccacac ggcggcgtgg cagttctggt tcggcctgct gttcatggcg
     3301 ctcatcctgt tctcaccgct cggcttcgtc ggcttgagcg agcgcctgac ggcgccgctg
     3361 cgccgcaagc gggtggacgc cgcggcgatg gccgcgcgcg tcacgcctca gcccggccag
     3421 gaggctccgg cgttcctgcg cgagcacccg cgcgtggacg gcgtgctgct cgaatgccgg
     3481 ggcgtgacca agcgcttcgg cgggttcacc gcggtggacg cggtggactt gcgggtccac
     3541 gatcgcacgc tgcacgcgct gatcggcccg aacggcgccg gcaagaccac gttgttcaac
     3601 ctcgtctcgg gcatgttcgc ccccgaccgc ggcaaggtgt cgctcgccgg cagaccgatc
     3661 caggggcgta cgcccgatgc ggtggccgcg cgcggactgt cgcgctcgtt ccagatcacc
     3721 agcctgttcc cctcgctcag cgtccgggag cacctgcgcc tgagcgtgca ggcgcgttcc
     3781 gagcggcgct atcacccgtt tcgcccggcc gattccctgg agcaggtcaa ccgcgagacg
     3841 ggcgagctgg tgcgcttcct cggcctggaa ggtttggagg aggtccccgt gtccagcctt
     3901 tcctacggcg ggcagcggct ggtcgagatc ggtgttgcgc tcgctgccaa gccgcgcgcg
     3961 ctgctgctcg acgagccgct ggcggggctg gccgccgccg agcgcagccg catcaccgcg
     4021 ctgatccgcg atctcacccg gcacatggcg gtgctgctgg tggagcacga catcgaccgg
     4081 gtgttcgcgg tggccgacaa gatcacggtg atgaacgagg gcaaggtgct ggtggaaggc
     4141 gatgcggaga cggtgcgcaa ccatcccgag gtgcagcgcg tgtacatcgg cgccggtcac
     4201 gcgcaggtgg cggcgctgga gcggccgcaa ccggccggcc ccggcgtacc gatcctgcag
     4261 gtccagggga tcaacacctt ctacggcaag agccacatcc tgcacgacgt ctcgttcgag
     4321 gtgcgcagcg gggaagtggt cgccttgctg gggcgcaacg gcgcgggcaa atcgtccacc
     4381 ttcaagagca tcatcggcat cgtcccgccg cgcagcggcg gcgtcaccct gaccggccgc
     4441 gaggtggccg gcatgacgcc ggagcgcatc gcacgcttgg gcgtaggcct ggtgccgcag
     4501 gggcggcggt tgttctcggg cctcaccgtc gatgagaacc tgcgcctggg cgcgctggcg
     4561 cgcgccggcg gcagcggcgt gcgctgggac cgcagccgca tctacggcta cttcccgcgc
     4621 gtgcgcgaca agctgcacac gcgcgccgat cagctctccg gcggcgagca gcagatggtg
     4681 gcgatcgccc gcgctctggt gggcaacgtc aggatcctgc tgctcgacga gcccttcgaa
     4741 ggtctgagtc cggccatggt ggaggaagtg ttccggtcga tcgagcagct gcgcaaggag
     4801 gtctcgatcc tgatcatcga gcatcacctc gacctggtgc tgtcgctcgc cgaccgcgcg
     4861 gtggtgctgg accgcggccg cgtgtcgcac gcggggccgg cggcgccgct gcggaccgat
     4921 ctcgatttcc gccggcaggt gttatggtta tgaactatcg tgcagggagt gacgaatgga
     4981 actgtcgatg aagaaccagc gcgtgctgat caccgcggcc gcgggcggga tcggccgggt
     5041 gatggcgcag acgttcgtgg aggccggcgc caaggtgcac atctgcgacg tggcgcagtc
     5101 ggccctcgac gagacggtga aggcgctgaa ggtgaccggc tcgcgctgcg acgtgtccga
     5161 ccgcaagcag gtggatctgc tgttcgccga cgcgaggcgg gatctcggcg ggctggacgt
     5221 gttggtgaac aacgccggca tcgccgggcc caccggcaag gtcgaggaga tcgcggtcga
     5281 ggactgggag cgctgcatcg ccgtggacct gaacggcatg ttctactgca cgcgcctggc
     5341 cgtgcccatg ctcaaggccg ccggcggcgg cagcatcatc aacttgtcgt cggccgccgg
     5401 gcggctggga ttcccgttcc gcacgccgta cgcggcggcc aagtggggtg tggtgggctt
     5461 caccaagagc ctgtcgatgg agctgggcac cgacaacatc cgcgtcaacg ccatccagcc
     5521 gggcgtggtg gagggcgagc gcatcgagcg cgtgatcgac gccaaggcca aggcgctggg
     5581 catcggcttc gaggagcaga agcggcgctc gctggacaag gtgtcgatgc gcaccatggt
     5641 gagcccgcag gacatcgcca acatggcgct ctacctggcg agcgcagccg ggcggcacgt
     5701 gaccgggcag gcgatctcgg tgtgcgccgg tgtcgagatg cttggatgaa aagcgttgag
     5761 atgctcggct gaaagggacg ggccatggaa aaaatcggaa tcgtgggcgc cgggctgatc
     5821 ggtcgcgcct gggccatcgt gttcgcgcgc gccggccatc ccgtgctgat gtacgacgcg
     5881 gacccgaagg ccgtgaaggc ggcgcagccg ctgatccgcg aggctctgca ggagttgcgc
     5941 gaggccggcc tggtgtcgga ggcggcggag gtcgtcgccc ggcgcatcgc gccggtggcc
     6001 acgctgcgcg actgcgtgca ggacgccgat tacgtgcagg agaacaccgc cgagcggctc
     6061 gaggtcaagc gcgaggtgta cgcgcagatg gacgcggccg cgaagccgga ctgcatcctg
     6121 gcgagctcga cgtcgaccat ccagacctcg cgcttcgccg acgggctgcc cggcagacag
     6181 cgctgcctgg tggcccaccc ggtcaacccg ccgcacgtgg tgccggtggt cgagatctcc
     6241 cccgccccgt tcaccgcgcc ggcagtcgtc gagcgcaccc gcgcgctgca cgccaaggtc
     6301 ggacaggtgc cgatcaccgt caagcgcgag gtggagggct tcatcctgaa ccgcctgcag
     6361 gccgcgctgc tgaacgaggc ctggcgcctg atcaaagagg actgttgcac ggtggaggac
     6421 ctggacaagt cgatcaagga cggcctgggg ctgcgctggg ccttcatggg gccgttcgag
     6481 accatcgatc tcaacgcgcc cggcggcatt ccggactatg ctgcgcgcta cgggtccaag
     6541 ctgcacggga tgatgcagga catccgctac gccccgtggg acgccgcgct cgtcgccaag
     6601 gtcgaggccg cgcggcgcgc aatcatgccg caagcgcagc acgccgcgcg cgaagcctgg
     6661 cgcgaccgga ggctgatggc gctgatcgcc cacaagcgcg cgatggctgc gaaagactga
     6721 agcatggatg gagcgagaaa tggcagagca acgaaaagtc atcatcacct gcgccgtgac
     6781 cggcgcgatc cacacgccga ccatgtcgcc gcatctcccg gtcacgccgg aggagatcgc
     6841 gcaaggcgcc atcggcgccg ccaaggccgg cgcggccatc gtccacctgc acgcgcgcga
     6901 gccgaaggac ggccggccca cgcaggaccc cgaggtgttc aagcgcttcc tgccggcgat
     6961 caaggcggcg agcgacgtgg tggtgaacct gaccaccggc ggcgccccca ccatgagcgt
     7021 ggaggaacgc ctgcagccgg ccctcaagct caagcccgag gtcgcctcgc tcatcatggg
     7081 ctcgatgaac ttcggcctgt acgagatgat cccgcgctac aaggagtgga agcac
//
LOCUS       DASPGE010000178        18308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_867710, whole genome shotgun sequence.
ACCESSION   DASPGE010000178 DASPGE010000000
VERSION     DASPGE010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18308
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867710"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VD791_05620"
     CDS             complement(<1..962)
                     /locus_tag="VD791_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXV07486.1"
                     /translation="MHPSIHARTTPDKIAYRMAASGECVTYRALDEASNRFAHLLRAH
                     GLAPGDHVALMLENHPRWFELCWGAQRAGLVFTPISTRLTAAEAGYILEDSGAKLFVT
                     SYALREVAAELRAQAPGVATRLMVDGGIDGYGSLERTLDRFPTRPVADETAGSDMLYS
                     SGTTGRPKGVLRGVASPAIDAPTPIAEVCSRLFGFDEHAVYLSPAPLYHAAPLSFCMT
                     VMRLGGTAIVMERFDAPEFLRLVQAHGVTHTQLVPTMFVRLLRLPPEERAHYDLSSLK
                     YAIHAAAPCPIPVKEQMLDWWGPIVWEYYAGTEGAGMTVVDPAS"
     gene            1103..1531
                     /locus_tag="VD791_05625"
     CDS             1103..1531
                     /locus_tag="VD791_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HXV07487.1"
                     /translation="MTERPLPAITPETRHFWEGTRAGELRLQRCGACHKAYFPPRPFC
                     PQCASADVAVFRASGRATLYSYVIHHRAAPGFAPPYSIAVVQLEEGPRMMTNIVETAQ
                     TPEALQLDMPLQVVFQRIDDDITLPLFRPAQSALQTGSAA"
     gene            1528..2670
                     /locus_tag="VD791_05630"
     CDS             1528..2670
                     /locus_tag="VD791_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HXV07488.1"
                     /translation="MKPGSVAVVGAAETTELGVIPHMSQIQLHADAARNALRDAGLKP
                     SDIDGVACAGEVPTNVAHYLGIVPKWVDGTAVGGCSFMLHVRHAAGAISAGLCKMVLI
                     THGESGRSRIGAIPRSVPPASLNGQFEAPFGTLGPPTMFTIPVLRYLKTYGVTQEQLA
                     MVAVVQREWAARNPRAMMRDPITVAEVLNSRMIAYPFRLLMCCLVTDGGGALVLTAAE
                     RARDLPQKPVYILGTGESVEGPMISQMEDFTSSRAFRVAGKEAFESADIRPADVDHLM
                     IYDAFAHLPLYGLEDLGFVGRGEAAAFIAERNTAPGGKLPVNTNGGGLSYTHTGMYGM
                     FALQESVRQVRGTAPAQVKDVQVAVCHGVGGMFAASGTIVMADRRP"
     gene            2680..3543
                     /locus_tag="VD791_05635"
     CDS             2680..3543
                     /locus_tag="VD791_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HXV07489.1"
                     /translation="MAIDYQKLKNWPFQDIVQTYTARDCILYALGVGLGADPLDRQQL
                     RFVYEDGLQMLPTMPVVLATPGFWLKDADTGIDWKTVLHGEQALTIHRPLPTAGTVRS
                     RMSIEEVVDKGPGKAAILYMRRDLDDHASGELLCSLWSSGVCRGAGGFGGPSGPTRAL
                     HPLPERKPDFACDLPTLQQAALIYRLSGDYNPLHADPVVAAAAGFERPILHGLCTYGV
                     AGHALLKTLCDYHGARLRRLDVRFSAPVYPGETIRTEIWKEGGGRASFRARSIERDIV
                     VLNNGVAEYEG"
     gene            3580..4404
                     /locus_tag="VD791_05640"
     CDS             3580..4404
                     /locus_tag="VD791_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV07490.1"
                     /translation="MGQLDGRVALVTGSGRGIGQQIALRLASDGARVVVNDLDEGPAR
                     DTIEQIESMGGRAVACNGDVTAADFGERIVKTAVDAFGGCHIVVNNAGYTWDNVIQKM
                     NDEQWYAILDVHLTAPFRILRAFVNHLRPAVEAEQKAGQRVVRKVVNISSTSGVNGNA
                     GQVNYSSAKAGIMGLTKALAKEWGRYSVTVNAVAFGLIQTRLTQPLQPGAAGTIDVQG
                     RTVKVGVQSDRLTALNQMIPLGRGGTPEEAAGAVYLFCSPDSDYVSGQTLVVNGGL"
     gene            4583..6376
                     /gene="aceK"
                     /locus_tag="VD791_05645"
     CDS             4583..6376
                     /gene="aceK"
                     /locus_tag="VD791_05645"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HXV07491.1"
                     /translation="MQETTSDLRGDLALAIARAMLEGFNKHYRLFRQASREAKQRFEA
                     ADWGAVRRAHAERILFYDERVKEAVERLQKEFDTDRLPDALWPEIKLQYVGLLTNHKQ
                     PELAETFFNTVSCKILHRTYYHNDFIFVRPAVSTEYLEGDPPSYRSYYPLKEGVRTVF
                     AQILRDFDLKVPFADLQRDLRYVLRALREKLPRRLRLDPNHQVQVLNSLFYRNQRAYM
                     IGRIINGGMEQPFAIPIVHEASGRGLCLDAMLLEPREIAPLFAFNRAYLMADMEVPSA
                     YVDFLSGMMPGKPKAELYTALGLQKHGKTLFYRDFLHHLNHSSDDFVIAPGIRGQVMI
                     VFTLPSYPYVFKVIRDSIAPSKNIDRQTVKEKYMLVKRHDRVGRMADTLEYTDVAFPR
                     ARFTGELIDELRREAPSLYEEDGDSIIVKHLYIERRMLPLNLYLDQAGERQTAAAIGD
                     YGNAIKELASVNIFPGDMLFKNFGVTRGGRVVFYDYDEIAYMSEINFRRIPPPRTPED
                     ELSAEAWYPVAPNDVFPEEFEPFLLSGDKVRRHFDGHRDLLTAEYWNAVKERLGRGQI
                     ADVFPYPDAVRFARRFAPRFTTGGCRFTTDR"
     gene            6387..7481
                     /locus_tag="VD791_05650"
     CDS             6387..7481
                     /locus_tag="VD791_05650"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HXV07492.1"
                     /translation="MAARLTTPLSERLRLEVPIFQAPMASVVTAALAAAVSKAGGLGA
                     FGFAFAQPDAMRKEVAALRAITDRPFQLNFFAAPQPAPVPADAQRRAIDALAGYYGEL
                     GLPPPQPAVAPYAPDLEAQLDTALELRPAAVTCHLDELPAERVRAFQGKGILVGGSAT
                     CVAEAKRLQELGMDFVVAQGGEAGGHRGTWTRDPKSALTGTLALVRLLVRAVELPVVA
                     AGGIMDGAGIAAVLSLGAQAAQLGTAFIPCPESAATETYREALLAAVEDDTDLTDKFT
                     GKPARGLRNRYLREAAERDLPRLPFPAQGHLTGQLRTASAQAGKPDFMTLWAGQAAPL
                     SRRLPAAELVRTLERETLEAIDRVARLRRG"
     gene            7563..8744
                     /locus_tag="VD791_05655"
     CDS             7563..8744
                     /locus_tag="VD791_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXV07493.1"
                     /translation="MNDPVVIVGAARTPMGAFQGELRDFTAPELGAVAIRAALERAGV
                     KSEDVEEVIMGCVLPAGQGQAPARQAALGAGLPLGTGCTTVNKMCGSGMKAAMLANDL
                     LLAGTNKVMVAGGMESMTNAPYLLPKARAGYRMGHQQVLDHMFLDGLEDAYDKGRLMG
                     TFAEDCAGKYGFTREAQDDFAVASLQRAQKANNEGWFAWETAHVALKSGKGDRFLDKD
                     EQPFKANFEKIPQLKPAFRKDGTVTAANSSSISDGAAALVLMRQSEAARRGLQPLARI
                     VAHSTHAQEPGWFTTAPVGAIRKLYDKTGWKPRDVDLYEINEAFAVVTLAAMKEHGLP
                     HDKVNVHGGACALGHPIGASGARIVVTLLGALRKYGLRRGVASLCIGGGEATALAVEL
                     S"
     gene            8752..9180
                     /locus_tag="VD791_05660"
     CDS             8752..9180
                     /locus_tag="VD791_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXV07494.1"
                     /translation="MSERKLISSGSTFEKIAGFSRAVVDGDWVFVAGTTGYDYSTMTI
                     APDVVAQTRQTFRNIDQALAQAGCTLKDVVRVHYYLTDQAYFKQVAPIFGEFLGDARP
                     AATAIVCRLVDDAIKIEIEVTARRAGAGVGARSDYPPGRT"
     gene            9191..9790
                     /locus_tag="VD791_05665"
     CDS             9191..9790
                     /locus_tag="VD791_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HXV07495.1"
                     /translation="MPTLDFYYDFSSPYGYFAATRISALAAEHGRGVNWHPILLGAVF
                     KVTGGAPLPSLPLKGPYGVKDMARTARFLGIPYSYPQPFPIPTQAPARIVYWLQATVP
                     EQVEPATLALYRAYFVDGRNIADPEVAADVVAALGIDRAAALAATNDQAMKDRLRNET
                     QAAIDRGVFGSPTVFIGEEQFWGVDRFDQIERWLREGGF"
     gene            9820..10407
                     /locus_tag="VD791_05670"
     CDS             9820..10407
                     /locus_tag="VD791_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HXV07496.1"
                     /translation="MTFQRLCIFCGSQIGHDPVYAGAAGDLGRLLAQRGIAVVYGGGH
                     VGMMGVLAEAALAAGGKVIGVIPEALKRRELAYENLTELIVTCTMHERKQRMADLSDG
                     FVALPGGFGTFEEFCEIVTWAQLGLHAKPCGLLNVNGYYDPMLAMFEHALREGFLRPQ
                     HRALVLTAHHADALLAAMQAWRPPPLEKWLTPETA"
     gene            10417..12024
                     /locus_tag="VD791_05675"
     CDS             10417..12024
                     /locus_tag="VD791_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXV07497.1"
                     /translation="MSSIRSNLDLRSAEFGASAAAMGALVADLRGKVELVKQGGGEPA
                     REKHAARGKLLPRERVRNLLDVGSPFLELSQLAAWGMYDNQVPAAGIITGIGRVSGRE
                     CMIVANDATVKGGTYYPMTVKKHLRAQEIAQQNRLPCLYLVDSGGANLPNQDEVFPDR
                     DHFGRIFYNQANMSALGIPQIALVMGSCTAGGAYVLAMSDESVIVKNQGTIFLGGPPL
                     VKAATGEVVSAEELGGADVHARTSGVADHYALNDLHALQIARRIVSHLNRAKRTELEV
                     REPREPAYDPAELHGVIPTDPRKPYDVREIIARIVDASELDEFKPLYGTTLVTGFAHI
                     CGYPVGIVANNGILFSESALKGAHFIELCSQRNVPLLFLQNITGFMVGRKYEAGGIAK
                     DGAKMVTAVATSRVPKFTVIVGGSFGAGNYGMCGRAYAPRFLWMWPNARISVMGGEQA
                     ASVLATVRREGAEKTGKTWSKRDEEAVKAPIRSQYEIQGHPYYASARLWDDGVIDPGD
                     TRRVLGLCISASLNAPIEPTQFGVFRM"
     gene            12095..12295
                     /locus_tag="VD791_05680"
     CDS             12095..12295
                     /locus_tag="VD791_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07498.1"
                     /translation="MRSGTLLFILFCLAIHGCGDQASSRQAEQTRIFDTQRDALDKAK
                     SVQDTVDRAAEQLRAQEAAQSD"
     gene            12460..13605
                     /locus_tag="VD791_05685"
     CDS             12460..13605
                     /locus_tag="VD791_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXV07499.1"
                     /translation="MTDTAYTTPEHLALREQVARFLAREVEPVAQAWEEQGYVPREVL
                     RKLGALGLLGITYPQEYGGAAADALTSLVFAEALSRTTFGGFAVTVLVHTDMASPHLV
                     HAGSPPQLERYLPKVISGELITAVAITEPDAGSDVKAIRTRARRSRGQWVLNGSKMFI
                     TNGVHADLYVVAARTDPDGQGAQALSMFLVEKGAPGFSVGRALKKQGWLCSDTAELVF
                     EDCRIPEDNLLGRENAGFYAIMKNFQTERIALSAMAVGHALTALDLTLGYVKQRRAFG
                     GALFEKQAIRQRLAMLQAKTEAARQFMYHCAWLVTQGRECVREVSMLKALTGELANEV
                     VYTCQQFHGGMGYMRETPIERLLRDARVLTIGGGATEVMLEEVAKRS"
     gene            13602..14420
                     /locus_tag="VD791_05690"
     CDS             13602..14420
                     /locus_tag="VD791_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXV07500.1"
                     /translation="MSTPDEAPVSPQAVLLHLDARGVATLTLNRPEIHNAFDDRLIVE
                     LAHKLREAANDPDVRAIVLAANGKSFSAGADLNWMKRMARYSEGENLRDAVGLADLMH
                     TISALPKPAVARVQGPAYGGGVGLVACCDIAVGSHAAAFSLSEVRLGLIPAVISPHVI
                     AAIGERQARRYFLTAERFDAGEALRIGLLHAVVEEAQLDAAVNAILDQLLKGGPKALA
                     AAKDLIASVANRPIDRRLVEDTAERIARIRVTAEGREGIAAFLEKRAPAWVKKT"
     gene            14428..16563
                     /locus_tag="VD791_05695"
     CDS             14428..16563
                     /locus_tag="VD791_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HXV07501.1"
                     /translation="MFDKVLIANRGEIAVRVARTCRRLGIHTVAVYSEADANAMHVAA
                     CDEAHLIGPAPARDSYLNAARIIEVAKRSRAQALHPGYGFLSENAGFAEACATAGLVF
                     IGPPAAAIRAAGSKSAAKALMEKAGVPLVPGYHGEAQDLDTLARAAQSVGYPVLIKAS
                     AGGGGKGMRVVEDAAQFESALASAKREAAAAFGDERVLIEKYLTRPRHIEMQVFADAQ
                     GNVVHLFERDCSVQRRHQKVLEEAPAPGMTAARRRLMGSAAVAAARAVGYVNAGTVEF
                     IADEAGDFYFMEMNTRLQVEHPVTEMVTGLDLVEWQLKIAAGEPLPLAQDQIALEGHA
                     IEARIYAEDPMRDFLPAAGRIQRLRAPQPSPHVRVDTGVREGDEIGVHYDPMIAKLVV
                     WDADRDAAVRRLRAALADYQIVGLTTNVQFLLAVAAHRAFAQAHREAGLLDTGLIARH
                     KGELLPEPQAASREILALAVLAELTRIDDQARALARASGDPWSPWHARDGWRLNEDNH
                     HVFTLRDGEREVAVTAHYRKGGFLLDLPVSGAAAGAQVLARAEAEPDGRLVADLGGAR
                     LHATVVRSGRVLTVFAGGVSHRLELKEFETVADEEAGGSLTAPMPGNVVQVLVKAGQR
                     VGKGQALMIIEAMKMEHTIAAPAEGAIARVLFARGDQVQEGDILFQFEPTVPSPDGQE
                     GPSSISGGEGRERGRDEQYLSPLAGAGPG"
     gene            16575..17501
                     /locus_tag="VD791_05700"
     CDS             16575..17501
                     /locus_tag="VD791_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HXV07502.1"
                     /translation="MQLPQRVKIVDVGPRDGLQNEPGSVPTEIKIGLIDRLSAAGLPA
                     VEATSFVSPKWVPQMADAPQVMAGIRRKPGVSYPVLVPNLKGLEGAMAAGAEEIAVFG
                     AATEAFSRRNTNCSIEESFERFEPVCRTALDNGLKVRGYISVALGCPFEGEVRPEAVA
                     TVAQRLDQMGCYEISLGDTIGAGTPGKTRRMIEAVARRIPVDRLGGHYHDTYGQALAN
                     ILASLEMGMGAFDSSVSGLGGCPYAPGATGNVATEDVLYMLDGLGIETGVDMGKLLEA
                     GEYICGFLNRPTASRAGRALLAKRKREAKAVA"
     gene            17549..17749
                     /locus_tag="VD791_05705"
     CDS             17549..17749
                     /locus_tag="VD791_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HXV07503.1"
                     /translation="MNQAPEHVPSPCAKVCALDPRSGYCVGCYRTIDEVANWVEMSAE
                     DKRAVLERVAQRRALQSGEVGR"
     gene            17746..>18308
                     /locus_tag="VD791_05710"
     CDS             17746..>18308
                     /locus_tag="VD791_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV07504.1"
                     /translation="MTKLLHGALGAMLALAVSVAGAQPAGRVTILYDAFGQKPGFTRD
                     WGFAALIEYGGRRILFDTGNDPHIFADNVRAAGVDLRRLDFVVISHRHLDHTAGIAHF
                     LSVNPDVTLYVLKEPFGVFGGALPSTFFRRDSSLAPEERYFGGQPPEELVSGTAFPEA
                     HWGYVDKTSEIAPGVYAIALVSDTPGTR"
BASE COUNT         2950 a         6180 c         6429 g         2749 t
ORIGIN      
        1 caggatgccg gatccaccac cgtcatgccc gcgccttccg tgccggcgta gtactcccac
       61 acgatcggac cccaccagtc gagcatctgc tctttaaccg ggatcgggca gggcgcggcg
      121 gcgtggatgg cgtacttcag cgacgacagg tcgtagtgcg cacgctcctc gggcggcaac
      181 cgcagcagcc gcacgaacat ggtcggcacc agctgcgtgt gcgtgacgcc gtgcgcctgc
      241 accaaccgca ggaattcggg cgcgtcgaag cgctccatca cgatcgcggt gccgcccagc
      301 cgcatcaccg tcatgcagaa gctcagcggc gcggcgtgat acagcggtgc gggcgagagg
      361 tacacggcgt gctcgtcgaa gccgaacagc cggctgcaca cctcggcgat cggggtgggc
      421 gcatcgatcg ccggcgacgc cacgccccgc agcaccccct tcggccggcc ggtggtgccc
      481 gacgagtaca acatgtcgct acctgcggtc tcgtcggcca ccggtcgggt gggaaaccga
      541 tcgagcgtcc gctcgaggct gccgtagccg tcgatgccgc cgtccaccat caagcgcgtg
      601 gccacgcccg gcgcctgcgc gcgcagctcc gccgcgacct cgcgcagcgc gtatgaggtg
      661 acgaacagct tcgcgccgct gtcctccagg atgtaaccgg cctcggcggc ggtgagccgg
      721 gtgctgatcg gcgtgaagac gagtcccgcg cgctgcgcgc cccagcacag ctcgaaccaa
      781 cgcgggtggt tctccagcat cagggcgacg tggtcgcccg gcgccagccc gtgcgcgcgg
      841 agcaggtgcg cgaaccggtt cgaggcctcg tccaacgcgc ggtacgtcac gcactcgccg
      901 cttgccgcca tgcggtaggc gatcttgtcc ggtgtggtgc gtgcgtggat cgacgggtgc
      961 atggtgttgc gcctcggctg cgccaaagta tatgcgttgc aggggcgcag atcagcagcg
     1021 cggaagcgcc aagcccgcgc ctctgatagc ctttgcccaa agcaccatgt cttgcccaaa
     1081 gcactgggga ggaggccccg ccatgacgga acgaccgttg ccggcgatca cgccggagac
     1141 ccgccacttc tgggaaggaa cccgagccgg cgagctgcgc ctgcagcgat gcggcgcttg
     1201 ccacaaggcg tacttcccgc cgcggccgtt ctgcccgcag tgtgcgtccg cggatgttgc
     1261 cgtgttccgc gccagcggcc gggcgacgct gtacagctac gtcatccacc accgcgccgc
     1321 gccgggcttc gcgcccccgt actcgatcgc ggtggtgcag ctcgaagagg gcccgcggat
     1381 gatgaccaac atcgtggaaa cggcgcagac ccccgaagcg ctgcagctcg acatgccgct
     1441 gcaggtggtt ttccagcgca tcgacgacga catcacgctg ccgctgttcc ggcccgccca
     1501 aagtgcactt caaaccggga gcgcggcatg aagcccggca gcgttgccgt cgtcggcgcc
     1561 gccgagacca ccgagctggg ggtgatcccc cacatgtcgc agatccagct gcacgccgac
     1621 gccgcgcgca acgcgctgcg cgacgcgggg ctgaagcctt ccgacatcga cggcgtggcc
     1681 tgcgcgggtg aggtgccgac caatgtcgcg cactacctgg gcatcgtgcc gaagtgggtg
     1741 gacggcaccg cggtgggcgg ctgctcgttc atgctgcacg tgcggcacgc ggctggtgcg
     1801 atcagcgccg ggctgtgcaa aatggtgctg atcacgcatg gagaaagcgg ccggtcgcgc
     1861 atcggggcga ttccgcgcag cgttccaccc gcgtccctga acgggcagtt cgaagccccg
     1921 ttcggcactc ttggtccacc caccatgttc accatcccgg tgttgcgtta tttgaagacg
     1981 tacggcgtca cccaggagca gctggccatg gtggcggtgg tgcagcgtga gtgggcggcg
     2041 cgcaacccgc gcgcgatgat gagggatccc atcacggtgg ccgaagtgct caactcgcgc
     2101 atgatcgcct atccgttccg gctgctgatg tgctgcctgg tcaccgacgg cggaggggcg
     2161 ctggtgctca ccgccgccga gcgtgcccgg gatctgccgc agaagccggt gtacatcctc
     2221 ggcaccggcg agagcgtcga gggtccgatg atcagccaga tggaggactt cacttcctcg
     2281 cgcgccttcc gcgtcgcggg caaggaagcg ttcgagagcg cggatatccg gcccgcggac
     2341 gtcgatcatt tgatgatcta cgacgccttc gcccacctgc ccctgtacgg gctcgaggac
     2401 ctcggcttcg tcggccgcgg cgaggccgcc gctttcatcg ccgagcgcaa caccgcgccc
     2461 ggcggcaagc tgccggtcaa caccaacggc ggtggcttga gctacacgca caccggcatg
     2521 tacggcatgt tcgcgctgca ggagagtgtg cgccaggtgc gcggcaccgc cccggcgcag
     2581 gtgaaggacg tgcaggtggc cgtgtgccac ggcgtgggcg ggatgttcgc cgccagcggg
     2641 accatcgtca tggcggatcg ccgcccctga gtcgcggaca tggcgatcga ctaccaaaag
     2701 ctgaagaact ggccgtttca ggacatcgtc cagacctaca cggccaggga ctgcatcctc
     2761 tatgccctgg gcgtcgggct gggtgccgat ccgctcgacc gccagcagct gcgcttcgtc
     2821 tacgaggacg gactgcagat gctgccgacg atgcccgtag tgctggccac tccgggcttc
     2881 tggctgaagg atgcggacac cggcatcgac tggaagacgg tgctgcacgg cgagcaggcg
     2941 ctgactatcc accgcccttt gcccaccgcc ggaacggtgc gcagtcggat gagcatcgag
     3001 gaggtcgtgg acaaggggcc gggtaaagcc gcgatcctgt acatgcgccg tgatctggac
     3061 gaccacgcca gcggcgagct gctgtgctcg ctgtggtcga gcggcgtctg ccgcggcgcg
     3121 ggcggcttcg gcgggccgtc cggtccgacg cgcgcgctgc atccgctgcc ggagcggaag
     3181 ccggatttcg cttgcgacct tccgacgctg cagcaggccg cgctgatcta ccgcctgagc
     3241 ggcgactaca acccgctgca cgccgatccc gtggtggctg cggcggccgg cttcgagcgg
     3301 ccgatcctgc atgggctgtg cacctacggg gtcgccggcc acgcactgct gaagacgctg
     3361 tgcgactacc acggcgcccg actgcgccgc ctggatgtgc gcttctccgc gcccgtgtac
     3421 ccgggcgaga ccattcgcac cgagatctgg aaggaaggcg gcggccgcgc cagcttccgc
     3481 gctcgctcga tcgagcgcga catcgtggtc ctgaacaacg gtgtggccga atacgagggc
     3541 tgatcgcgag taagacgctt gagcgaaggg agaaccagaa tgggacaact ggacggcagg
     3601 gtggcgctgg tgaccggatc gggtcgcggc atcggccagc agatcgcgct gcgcctggcg
     3661 agcgacgggg cgcgcgtggt ggtgaacgat ctcgacgagg gaccggcgcg cgacaccatc
     3721 gagcagatcg aatcgatggg cgggcgtgcg gtggcctgca acggcgacgt caccgcggcc
     3781 gacttcggcg agcgcatcgt gaagaccgcc gtggacgcct tcggcggctg ccacatcgtg
     3841 gtgaacaacg ccggctacac ctgggacaac gtgatccaga agatgaacga cgagcagtgg
     3901 tacgcgatcc tggacgtgca cctgaccgca ccgttccgca tcctgcgcgc gttcgtcaat
     3961 cacctgcggc cggcggtcga agccgaacag aaggccgggc agcgcgtagt gcgcaaggtg
     4021 gtcaacatct cgtcgacctc gggcgtcaac ggcaacgccg gccaggtcaa ctactcgtcg
     4081 gcgaaggccg gcatcatggg cctgaccaag gccctcgcga aggagtgggg acggtacagc
     4141 gtcaccgtca acgcggtggc tttcggcctg atccagacgc gactgacgca gccgctgcag
     4201 ccgggtgcgg ccggcaccat cgacgtccag ggccgcactg tcaaggtcgg cgtgcagagc
     4261 gaccgcctga cggcgctgaa ccagatgatt ccgctcggcc gcggcggcac cccggaagag
     4321 gccgccggcg ccgtgtacct gttctgcagc ccggacagcg actacgtcag cgggcagacg
     4381 ctggtggtga acggggggtt gtagcgcggc ggctgcgtcg cacgacgatc atgtaccggc
     4441 cggagcccgg gtcgcctgcc ggttgacact tctgcgaggt gtggcgatct cggccgggcg
     4501 ggcggtgccg gccaaacgtt tgatccggat cgtgctgccg tgcggtatca tcttcttgcg
     4561 gttcgatctc gagttgctcc ggatgcaaga gaccacctcc gaccttcgcg gcgatctggc
     4621 gctggctatc gcccgcgcga tgctcgaggg cttcaacaag cactaccgcc tgttccggca
     4681 ggcgagccgc gaggcaaagc agcgcttcga ggcggccgat tggggggcgg tgcgccgtgc
     4741 ccatgcggag cgcatcctct tctacgacga gcgggtgaag gaggcggtcg agcgcctgca
     4801 gaaggagttc gacaccgatc gcctgcccga tgcgctgtgg ccggagatca agctgcagta
     4861 cgtggggctg ctcaccaacc acaagcagcc ggagctggcc gagaccttct tcaacacggt
     4921 gagctgcaag atcctgcacc gcacctacta tcacaacgac ttcatcttcg tgcggccggc
     4981 ggtgtccacc gagtacctgg agggcgaccc gccgtcgtac cgcagctact acccgctgaa
     5041 ggaaggggtg cgcaccgtgt tcgcgcagat cctgcgcgac ttcgatctga aggtgccgtt
     5101 cgccgatctg cagcgcgacc tgcgctacgt gctgcgcgcg ctgcgcgaga agctgccgcg
     5161 gcgtctgcga ctggacccga atcatcaggt gcaggtgctg aattcgctct tctaccgcaa
     5221 tcagcgcgcc tacatgatcg gcaggatcat caacggcgga atggagcagc ccttcgccat
     5281 ccccatcgtg cacgaggcct ccgggcgcgg cctgtgcctg gacgcgatgc tgctcgagcc
     5341 gcgcgagatc gcgccgctgt tcgctttcaa ccgcgcctac ctgatggcgg acatggaggt
     5401 gccttcggcc tatgtcgatt ttctgtccgg catgatgccc ggcaagccca aggcggagct
     5461 gtataccgcg ctcgggctgc agaagcacgg caagacgctc ttctaccgcg acttcctgca
     5521 ccatctcaat cactctagcg acgatttcgt gatcgcgccc ggcattcgcg ggcaggtgat
     5581 gatcgtgttc acgctgccgt cgtacccgta cgtgttcaag gtcatccgcg acagcatcgc
     5641 gccttccaag aacatcgacc ggcagacggt gaaggaaaaa tacatgctgg tgaagcggca
     5701 cgaccgggtc gggcgtatgg ccgacacgct cgagtacacc gacgtggcgt tcccgcgtgc
     5761 gcgcttcacc ggggagctga tcgacgagct gaggcgcgag gccccttccc tgtacgagga
     5821 ggacggcgac agcatcatcg tcaagcacct gtacatcgag cggcgcatgc tgccgctcaa
     5881 tctgtacctg gaccaggccg gcgagcgcca gacggcggcg gcgatcggcg attacggcaa
     5941 cgccatcaag gagctggcct cggtgaacat cttcccgggc gacatgctgt tcaagaactt
     6001 cggcgtcacg cgcggcgggc gcgtggtgtt ctacgattac gacgagatcg cctacatgtc
     6061 ggagatcaac ttccggcgca tcccgccgcc gcgcacgccc gaggacgagc tgtccgcgga
     6121 ggcctggtat cctgtggcgc ccaacgacgt tttccccgag gagttcgagc cgttcctgct
     6181 gtccggggac aaggtgcggc ggcatttcga cgggcaccgc gacctgctca ccgcggagta
     6241 ctggaacgcg gtgaaggagc gcctaggccg cgggcagatc gcggacgtgt tcccgtatcc
     6301 cgatgcggtg cgcttcgcgc gtcgcttcgc cccccgcttc accactggcg gatgccgctt
     6361 cacgactgac agatgaagga gcgcccatgg cagcccgatt gacgacaccg ctcagcgagc
     6421 ggttgcggct cgaggtcccg atcttccagg ccccgatggc gagcgtggtc accgcagcgc
     6481 tcgcggcggc ggtgtcgaaa gccggtggcc tcggcgcttt cggctttgcc ttcgcccagc
     6541 ccgatgcgat gcgcaaggag gtggctgcct tgcgcgccat caccgatcgg ccgttccagc
     6601 tcaacttctt cgccgcgccg cagcccgcgc cggtgccggc cgatgcccag cggcgggcga
     6661 tagatgcgct ggcgggctat tacggcgagc tgggcctgcc accgccccaa cccgcggttg
     6721 cgccctacgc ccccgatctg gaagcgcagc tcgacaccgc gctggagctg cggccggcag
     6781 ccgtgacctg tcacctcgac gagctgcctg cggagcgcgt tcgcgcgttc cagggcaagg
     6841 gaattctggt cggggggagc gcaacctgtg ttgccgaggc gaagcggctg caggagctcg
     6901 gcatggactt cgtcgtggcg cagggcggcg aggccggcgg gcatcgcggc acctggacgc
     6961 gcgacccgaa gagcgctttg accggcacgc tggcgctggt gcggctgctg gtgcgcgcgg
     7021 tggagctgcc ggttgtcgcg gccggcggca tcatggacgg cgcgggtatc gcggcggtgc
     7081 tctcgctcgg cgcgcaggcg gcccagctcg gcacggcgtt catcccttgc ccagagagcg
     7141 cggcgaccga gacgtaccgc gaagcgctgc tcgccgcggt ggaggacgat accgacctca
     7201 ccgacaagtt caccggcaag cctgcccgtg gactgcgcaa ccgctaccta cgcgaagccg
     7261 cggaaaggga cttgccgcgg ctgccgttcc cggcgcaggg ccacctcacc gggcagctgc
     7321 gcaccgcgtc ggcgcaggcc gggaagcccg acttcatgac gctgtgggcg ggtcaggccg
     7381 cgccgctgtc gcgccgtctg cctgcagcgg aactggtgcg cacgctggag cgcgaaacgc
     7441 tggaagctat cgaccgcgtg gcgcgcctgc ggcgaggttg acgatggaag cggcagcgct
     7501 gcgtagcgcg caaaccactc cctctcctcg acgggagagg gttctcttct ggagcattgg
     7561 agatgaatga tcctgtcgtc atagtcggtg ccgcgagaac tcccatgggc gcctttcaag
     7621 gagaactcag ggatttcacc gcccccgagc tgggcgctgt cgccatccgc gccgcactgg
     7681 agcgcgcggg agtgaagtcc gaagacgtcg aagaggtgat catgggctgc gtcctgcctg
     7741 cagggcaggg ccaggcccct gcacggcagg ccgcgctcgg cgcgggcctg cccctgggca
     7801 ccggctgcac cacggtgaac aagatgtgcg ggtcgggcat gaaggcggcg atgctcgcca
     7861 acgacctgtt gctggccggc accaacaagg tgatggtcgc gggcggcatg gagagcatga
     7921 ccaatgcgcc gtacctgctg cccaaggcac gcgccggtta tcgcatgggt caccagcagg
     7981 tgctggacca catgttcctg gacggcctcg aggatgccta cgacaaaggc cggctgatgg
     8041 ggaccttcgc agaggactgt gcggggaaat acggtttcac gcgcgaggcg caggacgatt
     8101 tcgccgtggc gtcgctgcag cgcgcgcaga aggccaacaa cgaaggctgg ttcgcctggg
     8161 agaccgcgca tgtggcgctc aagtccggca agggcgaccg cttcctggac aaggacgagc
     8221 agccgttcaa ggccaacttc gagaagatcc cccagctcaa gccggcgttc aggaaggacg
     8281 gcacggtgac cgccgccaac tccagctcca tctccgacgg cgcggcggcg ctggtcctga
     8341 tgcgccagtc cgaggccgcg cgccgcgggc tgcagccgct ggcgcgcatc gtcgcgcaca
     8401 gcacgcatgc gcaggagccc ggctggttca ccacggcgcc ggtgggcgcg atccgaaagc
     8461 tctacgacaa gaccggctgg aagccgcggg acgtcgatct gtacgagatc aacgaggcct
     8521 tcgccgtggt gaccctggcg gcgatgaagg agcacggcct gccgcacgac aaggtgaacg
     8581 tgcacggcgg ggcgtgcgcg ctgggtcacc ccatcggcgc atcgggtgcg cgcattgtgg
     8641 tgacgttgct cggcgcgctg cgcaagtacg ggctgcggcg tggcgtggcg agcctgtgca
     8701 tcggcggcgg cgaggcgacc gcgctcgccg tcgaactgag ctaggactcc catgagcgaa
     8761 cgcaaactga tttcctccgg ctccaccttc gagaagatcg ccggcttcag ccgtgcggtg
     8821 gtggacggtg actgggtgtt cgtcgcgggc accaccggct acgactactc gaccatgacc
     8881 atcgcgccgg acgtggtggc tcagacccgg cagacgttcc gcaacatcga ccaggccctg
     8941 gcgcaggccg gctgcaccct gaaagacgtg gtgcgggtgc actactacct caccgaccag
     9001 gcgtacttca agcaggtggc gccgatcttc ggcgagttcc tgggcgacgc gcgaccggcg
     9061 gcgacggcga tcgtctgccg cctggtcgat gacgcgatca agatcgagat cgaagtgacc
     9121 gcgcggcgcg cgggcgcagg cgtcggcgcg cgcagcgact acccgcccgg gcgtacctag
     9181 acaatccatc atgcccaccc tcgacttcta ctacgacttc tcctcgccct acggctattt
     9241 cgccgcgacc aggatttcgg cgttggctgc ggagcacggg cgcggcgtga actggcaccc
     9301 gatcctgctc ggcgcggtgt tcaaggtgac cggcggcgcg ccgttgccca gcctgccgct
     9361 gaagggccct tacggcgtga aggacatggc gcgcactgcg cgcttcctgg gtatccccta
     9421 cagctatccc cagccgttcc cgattccgac ccaggcgccg gcgcgcatcg tctactggct
     9481 gcaggcgacg gtgcccgaac aggtcgagcc ggccacgctg gcgctgtatc gcgcgtactt
     9541 cgtcgacggt cgtaacatcg ccgatccgga agtcgccgcg gacgtcgtcg ccgcgctcgg
     9601 catcgaccgt gccgccgcgc tggccgccac caacgaccag gcgatgaagg atcgcttgcg
     9661 caacgagacc caggcggcca tcgaccgcgg cgtgttcggc tcgcccaccg tgttcatcgg
     9721 cgaggagcag ttctggggcg tcgatcgctt cgaccagatc gagcgctggt tgcgggaagg
     9781 cgggttctga gcgccgccgc gcgcgcgtcg cgggaggcaa tgaccttcca gcgcctgtgc
     9841 atcttctgcg gctcgcagat cggccacgac ccggtgtacg cgggcgctgc cggcgacctc
     9901 ggccgcctgc tcgcgcagcg cggcatcgcc gtggtgtacg ggggcggcca cgtcggcatg
     9961 atgggcgtgc tggcagaggc ggcgctggcc gccggcggca aggtcatcgg cgtgattccc
    10021 gaagcgctca agcgtcgcga gctcgcgtac gagaatctca ccgagctgat cgtcacctgc
    10081 accatgcacg agcgcaagca gcgcatggcg gacctgtccg acggcttcgt cgcgctgccc
    10141 ggcggtttcg gcaccttcga ggagttctgc gagatcgtca cctgggcgca gctcggcctg
    10201 cacgccaagc cctgcggcct gctcaacgtc aacggctact acgacccgat gctcgccatg
    10261 ttcgagcacg ccttgaggga aggtttcctg cgcccgcagc accgcgcgct ggtgctgacc
    10321 gcgcaccatg ccgatgcgct gctcgcggcg atgcaagcct ggcgcccgcc gccgctcgag
    10381 aagtggctca ctccggaaac ggcataatag gcggcgatga gcagcatccg ctccaacctc
    10441 gatctacgct ccgccgagtt cggcgccagc gccgccgcca tgggcgcgct ggtcgccgat
    10501 ctgcgcggca aagtcgagct ggtcaagcag ggcggtggcg agccggcgcg cgagaagcac
    10561 gcggcgcgcg gcaagctgct gccgcgcgag cgcgtgcgca acctgttgga cgtgggctcg
    10621 ccgttcctcg agctctccca gctcgcggcc tgggggatgt acgacaacca ggtgccggcc
    10681 gcgggcatca tcaccggcat cggccgcgtc agcgggcgcg agtgcatgat cgtggcgaac
    10741 gacgccacgg tgaagggcgg cacctattac ccgatgacgg tgaagaagca cctgcgcgcg
    10801 caggagatcg cgcagcagaa ccgcttaccc tgcctgtacc tggtggactc gggtggcgcc
    10861 aacctgccca accaggacga ggtattcccg gaccgcgatc acttcggccg catcttctac
    10921 aaccaggcca acatgtcggc cctgggcatt ccgcagatcg cgctggtgat gggttcctgc
    10981 accgcgggcg gcgcgtacgt gctggcgatg tccgacgaat cggtcatcgt caagaaccag
    11041 ggcaccatct tcctgggtgg cccgccgttg gtgaaagcgg ctaccggcga ggtggtgagc
    11101 gccgaagagc tgggcggcgc ggacgtgcac gcgcgcacct ccggcgtcgc cgatcattac
    11161 gccttgaacg acctgcacgc gctgcagatc gcacgacgca tcgtgtcgca cctaaaccgc
    11221 gccaagcgca cggaactcga ggtgcgcgag ccgcgggaac cggcgtacga cccggccgag
    11281 ctgcacggcg tgatccccac cgacccgcgc aagccctacg acgtgcgcga gatcatcgcc
    11341 cggatcgtgg acgccagcga gctggacgag ttcaagcctc tctacggcac gacgctggtc
    11401 accgggttcg cgcacatctg cggctacccg gtcggcatcg tcgccaacaa cggcatcctg
    11461 ttctccgagt ccgcgctcaa gggcgcgcac ttcatcgagc tgtgcagcca gcgcaacgtc
    11521 ccgctgctgt tcctgcagaa catcaccggc ttcatggtgg gccgcaagta cgaagccggc
    11581 ggcatcgcca aggacggcgc caagatggtc accgcggtcg ccacttccag ggtgcccaag
    11641 ttcaccgtga tcgtcggcgg cagcttcggt gccggcaact acggcatgtg cggccgcgct
    11701 tacgcccccc ggttcctgtg gatgtggccc aacgcgcgca tctcggtgat gggcggggag
    11761 caggccgcga gcgttctcgc caccgtgcgc cgcgaaggag cggagaagac gggcaagacc
    11821 tggagcaagc gcgacgagga agcggtcaag gcgccgattc gcagccaata cgaaatccag
    11881 gggcacccct actacgctag cgcccgcctg tgggacgacg gggtgatcga tcccggcgac
    11941 acgcggcggg tgctgggtct gtgcatctcc gcgagcctca acgccccgat cgaacccacg
    12001 cagttcggcg tgttccggat gtagccccgg cccgcggata cgggcctgcc ccttgcttcg
    12061 cgagtcgcca ccatctgtcc ccaggaggct acgcatgcgc agcggcacac tccttttcat
    12121 cctgttctgc ctggccatcc acggctgcgg cgatcaggcg tcgtctcgcc aggccgagca
    12181 gacgcggatc ttcgacaccc agcgcgacgc cctggacaag gccaagtcgg tgcaggacac
    12241 ggtggatcgg gcggccgagc agctccgcgc ccaggaggcg gcgcagagcg actagcaagg
    12301 agtagcgcgc ggcaatccga gctcgccggc cgcgcgaccg caggaccgct gcggacccgc
    12361 cagcatataa ttgcggatgg gccaagggca gcggagaacg ccgcgctgta tcgtcaccgc
    12421 cggtcgcaac ggcgcgggca agctgacgcc tcgaatctcg tgaccgacac cgcttacacg
    12481 actcccgagc acctcgcgct gcgcgagcag gtggcgcgct ttctcgcgcg cgaagtcgag
    12541 ccggtggccc aagcctggga agagcagggt tacgtgccgc gcgaggtgct gcgcaagctc
    12601 ggtgccctgg ggctgctcgg catcacctat ccccaggaat acggcggcgc ggccgccgac
    12661 gcgctgactt cgctggtgtt cgccgaagcg ctatcgcgca cgacgttcgg cggctttgcc
    12721 gtgaccgtgc tggtgcacac cgacatggcc tcgccgcacc tggtgcacgc cggctcgccg
    12781 ccgcagctcg agcgctatct gcccaaagtg atctcgggcg agctgatcac cgcggtggcg
    12841 atcaccgagc cggacgccgg gtcggacgtg aaggcgatcc gcacgcgcgc gcggcgcagc
    12901 cgcgggcagt gggtgctgaa cggcagcaag atgttcatca ccaacggcgt gcacgcggac
    12961 ctgtacgtcg tcgcggcgcg taccgacccg gacgggcagg gcgcgcaggc gttgtcgatg
    13021 ttcctggtgg agaagggcgc gcccggattc agcgtcggcc gcgcgctgaa gaagcagggg
    13081 tggttgtgct cggacacggc cgagctggtg ttcgaggact gccgcatccc ggaagacaac
    13141 ctgctggggc gcgagaacgc gggcttctat gccatcatga agaacttcca gaccgagcgc
    13201 atcgcgctgt cggccatggc ggtggggcat gcgctgacgg cgctcgactt gacgctcggc
    13261 tacgtgaagc agcggcgcgc cttcggcggc gcgctgttcg agaagcaggc catccgccag
    13321 cggctggcca tgctgcaggc gaagaccgag gccgcgcgcc agttcatgta tcactgcgcc
    13381 tggctggtca cgcaggggcg ggagtgcgtg cgcgaggtgt cgatgctcaa ggcgctcacc
    13441 ggcgagctcg ccaacgaggt cgtctacacc tgccagcagt tccatggcgg catgggctac
    13501 atgcgcgaga ccccgatcga gcggctgttg cgcgacgcgc gtgtgctcac gatcggcggc
    13561 ggcgcgaccg aggtcatgct cgaggaggtg gcaaagcgct catgagcacc cccgacgaag
    13621 caccggtaag cccgcaggcg gtcctgctgc acctggacgc gcgcggcgtg gccacgctca
    13681 ccttgaaccg ccccgagatc cacaacgcct tcgacgaccg cctgatcgtg gagctggccc
    13741 acaagctacg cgaggccgcg aacgatcccg acgtgcgcgc gatcgtgctg gcggccaacg
    13801 gaaagagctt ctccgccggc gccgatctga actggatgaa gcgcatggcg cgctactcgg
    13861 agggcgagaa tctgcgcgac gcggtagggc tggcggacct gatgcacacc atcagcgcgc
    13921 tgcccaagcc ggcggtcgcg cgcgtgcagg gcccggccta cggcggcggc gtcggcctgg
    13981 tggcgtgctg tgatatcgcg gtgggcagcc acgccgcggc gttctcgctg tcggaggtgc
    14041 ggctgggctt gattccggcg gtgatcagcc cgcacgtcat cgccgccatc ggcgagcggc
    14101 aggcgcggcg ctacttcctc accgccgagc gcttcgacgc tggggaagcc ctgcgcatcg
    14161 ggctgctgca cgcggtggtc gaggaagcgc agctcgacgc ggcggtgaac gccatcctcg
    14221 accagttgct caagggcggg ccgaaggcgc tggccgccgc gaaggacttg atcgcgtcgg
    14281 tggccaaccg ccccatcgat cgccgcctgg tcgaggacac ggccgagcgc atcgcccgca
    14341 tccgcgtgac cgccgaaggg cgcgagggca tcgcggcgtt cctcgaaaag cgcgcgccgg
    14401 cgtgggtgaa gaagacttag cgaactggtg ttcgacaagg tcctcatagc caaccgcggc
    14461 gagatcgcgg tgcgcgtcgc gcgcacctgc cggcggctgg gcatccacac ggtcgcggtg
    14521 tattcggagg ccgacgccaa cgccatgcac gtcgccgcct gcgacgaggc gcacctgatc
    14581 gggcccgccc cggcgcggga cagttatctc aacgccgcgc gcatcatcga ggtggccaaa
    14641 cgcagccgcg cgcaggccct gcatcccggc tacggcttcc tgtccgagaa cgccggcttc
    14701 gccgaggcct gcgccacggc cggtctggtg ttcatcgggc cgcccgccgc ggcgatccgc
    14761 gcggcgggct cgaagagcgc ggccaaggcg ctgatggaga aggccggagt gccgctggtg
    14821 ccgggctacc acggcgaggc gcaggacctg gacacgctgg cgcgcgcggc gcagagcgtc
    14881 ggttacccgg tgctgatcaa ggcctccgcc ggcgggggcg ggaagggcat gcgcgtggtc
    14941 gaggatgcgg cgcagttcga gagcgcgctc gcctccgcca agcgcgaagc cgccgccgcc
    15001 ttcggcgacg agcgcgtgct gatcgagaag tatctgacgc gcccgcgcca catcgagatg
    15061 caggtgttcg ccgacgcgca gggcaacgtc gtgcatctgt tcgagcgcga ctgctccgtg
    15121 cagcggcgcc accagaaggt gctggaggaa gctcccgccc ccggcatgac ggcggcacgc
    15181 cggcgcctga tgggatcagc cgccgtcgca gcggcgcgcg ccgtgggcta cgtgaacgcg
    15241 ggcacggtgg agttcatcgc cgacgaggcg ggcgacttct acttcatgga gatgaacacc
    15301 cggctgcagg tggagcaccc ggtcaccgag atggtcaccg ggctggacct ggtcgaatgg
    15361 cagctcaaga tcgccgccgg cgagccgctg ccgctcgcgc aggaccagat cgcgctggag
    15421 ggccatgcca tcgaggcgcg catctacgcc gaggacccga tgcgcgattt cctgccggcg
    15481 gcgggccgca tccagcgcct gcgcgcgccg cagccctcgc cgcacgtgcg cgtggacacg
    15541 ggcgtgcgtg aaggcgacga gatcggcgtg cactacgacc cgatgatcgc caagctggtg
    15601 gtgtgggatg cagaccgcga cgcggccgtg cggcgcctgc gcgcggcact cgccgactac
    15661 cagatcgtgg gcctcaccac caacgtgcag ttcctgctcg ccgtggctgc gcaccgcgcc
    15721 ttcgctcagg cgcaccgcga ggccggtttg ctggacaccg gcttgatcgc acgccacaag
    15781 ggcgagctgc tgcccgagcc gcaggccgcc tcgcgcgaga tcctcgcgct cgcggtgctc
    15841 gccgagctga cgcgcatcga cgaccaggcg cgcgcgctgg cccgggcctc cggcgatccc
    15901 tggtcgccct ggcacgcccg ggacggctgg cgcctgaacg aggacaatca ccacgtgttc
    15961 acgctgcggg atggagagcg cgaggttgcg gtcaccgcgc attaccggaa gggcgggttc
    16021 ctgctggatc tgcccgtatc tggagcggct gcaggtgcgc aagtgctcgc ccgcgccgag
    16081 gcggagccgg acgggcgcct ggtcgccgac ctgggcggcg cccgcctgca cgccaccgtg
    16141 gtgcgcagcg gccgggtgct gacggtattc gctggcgggg taagccatcg gctggaactg
    16201 aaggagttcg agacggtcgc cgatgaagag gccggggggt cgctgaccgc gcccatgccg
    16261 ggcaacgtgg tgcaggtgct ggtgaaggcg gggcagcggg tgggaaaggg ccaggcgctg
    16321 atgatcatcg aagcgatgaa gatggagcac acgatcgccg cgcccgccga gggcgcgatc
    16381 gcgcgggtgc tgttcgcgcg cggcgaccag gtgcaggaag gcgatatcct gttccagttc
    16441 gaaccgacgg tcccctctcc cgacggacaa gaaggtccct cttccatttc ggggggtgag
    16501 ggtcgggagc ggggccggga tgagcaatac ctctctcccc ttgcgggagc ggggccgggg
    16561 tgaggggtaa cacaatgcaa ctgccacaac gcgtgaagat cgtcgacgtc ggcccgcgcg
    16621 acgggctgca gaacgaaccc ggcagcgtgc caaccgagat caagatcggc ctgatcgacc
    16681 ggctcagcgc ggccggttta ccggcggtcg aggcgaccag cttcgtgtcg ccgaagtggg
    16741 tgccgcagat ggccgatgcg ccgcaggtga tggccggcat ccggcgcaaa ccgggcgtga
    16801 gctatccggt gctggtgccc aacctgaaag gcctggaggg ggcgatggcg gccggcgccg
    16861 aggagatcgc cgtgttcggt gccgccaccg aagcgttctc ccgccgcaac accaactgct
    16921 cgatcgaaga gtcgttcgag cgcttcgagc cggtgtgccg caccgcgctc gacaacggcc
    16981 tgaaggtgcg cggctacatc tccgtggcgc tgggctgccc gttcgagggc gaagtgcggc
    17041 ccgaggcggt ggccacggta gcgcagcgcc tggaccagat gggctgctac gagatcagcc
    17101 tgggcgacac catcggcgcg ggcaccccgg gcaagacgcg gcgcatgatc gaagcggtcg
    17161 cgcgccgcat tcccgtggac agactgggcg ggcattacca cgacacctac ggccaggcgc
    17221 tcgccaacat cctggcatcg ctggagatgg gcatgggcgc gttcgacagc tcggtgtccg
    17281 gcctgggggg ctgcccatac gcgccgggcg cgaccggcaa tgtcgccacc gaggacgtgc
    17341 tgtacatgct cgacggactc ggcatcgaga ccggcgtgga catgggcaag ctgctggagg
    17401 ccggcgagta catctgcggt ttcctcaacc ggccgaccgc ttcgcgcgcc ggccgggcgc
    17461 tgctggcgaa acgcaagcgc gaggccaagg cggtcgcgtg agccccggtg tcgcgtgagc
    17521 cccggtgtcg catgaacccc ggtgtcgcat gaaccaggcg cccgagcacg ttccctcgcc
    17581 ctgcgccaag gtgtgtgcgc tcgacccgcg cagcggctac tgcgtcgggt gctatcgcac
    17641 catcgacgag gtggccaact gggtcgagat gagcgccgaa gacaagcgcg ccgtgctcga
    17701 acgcgtggcg caacggcgcg cgctgcagtc gggggaagtg ggccgatgac gaagttgctg
    17761 cacggcgcgc tcggagcgat gctggccctg gccgtctctg tcgccggggc ccaaccggct
    17821 gggcgcgtga ccatcctgta cgacgccttc ggccagaagc cgggattcac ccgcgactgg
    17881 ggcttcgctg cgctgatcga gtacggcggc cggcgcatcc tgttcgatac cggcaacgac
    17941 ccgcacatct ttgccgacaa cgtccgtgct gcgggcgtgg atctgcgccg gctcgatttc
    18001 gtcgtgatct cccatcggca tctcgatcac acggccggca tcgcacactt tctgagcgtc
    18061 aacccggatg tcacgctcta tgtgctgaag gagccgttcg gagtcttcgg cggagcgctc
    18121 ccaagcacct tcttccgccg cgacagctcg ctcgcgcccg aagagcgata cttcggcggg
    18181 cagccgcccg aggagctggt gtcgggcacc gcgttcccgg aggcccactg gggttacgtc
    18241 gacaagacca gcgagatcgc gcccggggtg tacgccatcg cgctggtgtc ggacacgccc
    18301 ggcactcg
//
LOCUS       DASPGE010000179         7619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1335128, whole genome shotgun sequence.
ACCESSION   DASPGE010000179 DASPGE010000000
VERSION     DASPGE010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335128"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..986)
                     /locus_tag="VD791_05715"
     CDS             complement(126..986)
                     /locus_tag="VD791_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07505.1"
                     /translation="MTARTAATALLALALAACGEGPKDIQFAKYGEPYATKPDFAWIE
                     HKYPLTPTHRAQITPKYLAGLDQEQVDQIYARLTAGPIPDGPFDGDLFLPKGASGERR
                     VAEIIGGLKGFAVNLKGRKVEGLGRTLWKGKVFYRDERVLRNRIQDLALLAPLVDGPL
                     GDIPKIEVGGRDAWLLFPAKLYCGQSLLDSRRESIVIDYAFTDQIEGYREKPDFLAGR
                     RGLKIRDEIRMVRPGFYLGRAYADRAFLLNFTLYNKEMAERDGEAFANTGTSSEDCFI
                     GTQRAVVAAN"
     gene            complement(1006..3567)
                     /locus_tag="VD791_05720"
     CDS             complement(1006..3567)
                     /locus_tag="VD791_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07506.1"
                     /translation="MRTAGFLGLVIVVLAAAWWVIADPPADWPADWIAVPQAVAQSPA
                     APEPAAKSGTEAIYADAGFRLGEAALSPSEIAGREIWYKATAGNARFHTYVFQQRMGV
                     LIDWYRVLRSDQRGQRFRTWGLINDPDCCTPGSAGCPAKSLEETYGFDYCLGDAELLQ
                     YVGKEGYRDPACEMKDPPLAEGDVHGPGDQRHSSCDLAFGTSTGALGFRKFPNPRFDA
                     EQWRMLNGGKLGTWEGFNRRLSSDPNTTDGRTSRLSDGAVEPPFLIGTSCGSCHIAFD
                     PLDPPKDPERPQWRNLKGGIGNQYLRISELMVSGMPADSPEWQIFAHARPGTSDTSAI
                     PTDQVNNPGTINALINIKQRLTHDGEDIVKWRKAASCEQKEIDQDTCWCEPGRQNKCW
                     RKGRAQEAVHHILKGGEDSIGALEAIQRVYFNIGSCAEACWLNHLTDLRQVDPNQRNF
                     GQTPFDIGQCRRDCPNFRAIEDRLPDILAFLTSKETDATDLHVARANALRAKNPSASY
                     EYDDLVEDLEREFGDGAVRRGRAVFAENCALCHSSIPVEKGGEFANRDFRAVSRETGL
                     REDWMGSDASTPVSEVDTYRCRALHSNHMAGHIWQEYGSETLRARKQVPGIEEPSDGG
                     RGYYRNISLLSVWAHAPFMHNNAVGPELCGWGKPGPYELYRSSYVDANGKPLPASRQP
                     ACWAYDPSVEGRFKLYKESMRQLLDPHAREREHPKVTLLDQDIVLDVGPKVFDGEDEK
                     NLFGLTIVVPKGAKAGALGNFQHKQFVIDMVLAKIDRAKLEQRRGKEVAGLVAQAADE
                     ILQDPKRMVDALRSRPQLIAVYSSCDDLIENKGHRFGEDLPESDKKALIAFLATL"
     gene            3852..6974
                     /locus_tag="VD791_05725"
     CDS             3852..6974
                     /locus_tag="VD791_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXV07507.1"
                     /translation="MARKKTTREPAPRRKRSTAAAAAPVPPSDAASAFVMDNDEIERA
                     LRTGQDAGALEDYFGPRDYAELRELAQQSAAKRAAAGPKVLILPGITGSRLGQIAVNG
                     QENIVWFDPVELAFGAIRKLAVGSGVKIVPLGVILLYYLKLKLRLRALGFDADFYPYD
                     WRQSLDTLGAQLAERIRNAPALDGVSLVCHSMGGLVARAALAALGAAGKKIGRVVQLG
                     TPNFGSFCAVQTMRAQGDTVKKLAFLDQRHDAQEIVADVLAPMPGFCQLLPSPERFNA
                     IDLFDARRWPASRPLPQAEVLRQVPGVLKRLAPADERFTLIAGINRETVTGLRKDEQK
                     DEFVFQLSMAGDGTVPLALAELPGVKATYYIEETHGSLPNNKIVARAVADILSTGATA
                     LLPSTVGAVRREVTREVSESALATQQPYPGRRQGRLSQRELREVLSEFVSTETRDAEQ
                     PLAMTAAGAAAPEADLAQRFDRLVIGRRRQHRMDLRLALGDVGDVNTQALVIGIYRSV
                     APSGAAKAIDARLDGAITELTQRRMFSGNVGEVFLLPTAQRAVRADMVGFIGLGAFDT
                     FTEEVLRIAAENAIRTLVLGGVYDLATVLFGGGSGAGVRSAVENLLSGFVKGLMDADA
                     GHRFRCLTVCEGNPERYEEIKRELFRLSSTPLFDELEVTFFEERLPPAVAPAGRRAAA
                     RPDPVYLLVRQEGKTGSGLELRTSVLGAGGKATVVSGVRQLKRIDLDGKLDELQTSGF
                     RFDRAPAYGRELSNLVVSDEALTALAALKDRHVVVVHDAESSRIPWELLAVPGSAQAT
                     TLLAKEGGLSRRYVADNLSVAKWLEERRQDLELNLLLVVNPTLDLDGAEEEGRRIREL
                     LAGEPAVRVDELRGRDASRPALERAFRSGKYDVIHYAGHAFFDEADPAASGILCAGKA
                     VLSGRDLAGLGNLPALVFFNACEAGRVRRAGRAAQAAPSMRERIDRNVGLAEAFLRGG
                     VANYVGTYWPVGDEPAKLFAEQFYRSLMQGQSIGDALLAGRRTVWERKSVDWADYIHY
                     GSHDFVLKTR"
     gene            7281..>7619
                     /locus_tag="VD791_05730"
     CDS             7281..>7619
                     /locus_tag="VD791_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07508.1"
                     /translation="MQRENSISVSAPGVRVRSRRSFLKGSGLLAGGIITSATLAGLAA
                     HSAQAHAGEDRDHRECRRARGRRSDYGKLTPTPDQDGAHILALPRGFHYVTFSKTGEV
                     FGDGLLVPRNH"
BASE COUNT         1131 a         2591 c         2682 g         1215 t
ORIGIN      
        1 cccggggcgg caggcgccgt ggtccaggcg ctttccgggg gcagctcctg tgcattcagc
       61 gcgggcgacg agcagaggaa tgcagcgagc aggcgcgtac ggagtcggcg cggacccggc
      121 acgcgtcagt tcgccgccac cacggcgcgc tgcgtgccga tgaagcagtc ctcgctgctc
      181 gtgccggtgt ttgcgaacgc ctcgccgtcg cgttcggcca tctccttgtt gtacagcgtg
      241 aagttgagca ggaacgcgcg gtccgcgtag gcgcggccga gatagaaccc ggggcgcacc
      301 atgcggatct cgtcgcggat cttcagcccg cgccggccgg ccagaaaatc cggtttctcg
      361 cgatacccct cgatctggtc ggtgaaggcg taatcgatga cgatcgactc gcggcgcgag
      421 tcgagcaggc tctgtccgca gtagagcttg gcggggaaca gcagccaggc gtcgcggccg
      481 ccgacctcga tcttgggaat gtcgccgagc ggcccgtcca ccagcggggc gagcagggcc
      541 aggtcctgga tgcgattgcg cagcacgcgc tcatcacgat agaacacctt gcccttccac
      601 agcgtgcggc cgaggccctc caccttgcgg cccttgaggt tgacggcgaa gcccttgagc
      661 ccgccgatga tctcggccac gcgccgctcg ccgctcgcgc ccttgggcaa gaacaggtcg
      721 ccgtcgaagg ggccgtccgg gatcggcccg gcggtaagcc gcgcatagat ctggtcgacc
      781 tgctcctgat cgaggccggc gaggtacttg ggggtgatct gcgcgcggtg cgtcggcgtg
      841 agcggatact tgtgctcgat ccaggcgaag tcgggcttgg tcgcgtaggg ctcgccgtac
      901 ttggcgaact ggatgtcctt ggggccttcg ccgcaggcgg caagcgccag ggcgagcagc
      961 gcggtggcgg cggttctcgc agtcatggtc gtcctcctcc gcggctcaca gcgtggccag
     1021 gaaggcgatg agcgccttct tgtcggactc cggcaggtct tcgccgaagc ggtggccctt
     1081 gttctcgatc aggtcgtcgc agctgctgta caccgcgatc agctgcgggc ggctgcgcag
     1141 ggcatccacc atgcgcttgg gatcctgcag gatctcgtcg gccgcctgcg ccaccagccc
     1201 ggcgacttcc ttgccgcgcc gctgctcgag cttggcgcgg tcgatcttgg cgagcaccat
     1261 gtcgatcacg aattgcttgt gctggaagtt gccgagcgcg ccggccttgg cgcccttggg
     1321 caccacgatg gtgagcccga acaggttctt ctcgtcctcg ccgtcgaaca ccttggggcc
     1381 cacgtcgagc acgatgtcct ggtccaacag cgtcaccttg ggatgctcgc gttcgcgcgc
     1441 gtgcgggtcg aggagctggc gcatggattc cttgtacagc ttgaagcgcc cttcgacgct
     1501 gggatcgtag gcccagcagg cgggctgccg cgacgccggc agcggcttgc cgttcgcgtc
     1561 cacatacgag gagcggtaca gctcgtaggg gccgggtttg ccccagccgc acagctccgg
     1621 gcccaccgcg ttgttgtgca tgaacggcgc gtgcgcccac acgctcagca gcgagatatt
     1681 gcggtagtag ccgcgcccgc cgtccgaggg ctcttcgatc ccgggcacct gcttgcgcgc
     1741 gcgcaacgtc tcggagccgt actcctgcca gatgtggccg gccatgtggt tcgagtgcag
     1801 cgcgcggcag cgataggtgt ccacctccga caccggggtg gacgcgtcgc tgcccatcca
     1861 gtcctcgcgc agaccggtct cgcgcgagac cgcgcggaaa tcgcggttgg cgaactcccc
     1921 gcccttctct acggggatgc tggaatggca gagcgcgcaa ttctccgcga acaccgcacg
     1981 gccgcgcctc accgcaccgt ccccgaactc gcgttcgagg tcctccacca ggtcgtcgta
     2041 ctcgtagctc gcgctggggt tcttcgcacg cagcgcgttg gcgcgggcga cgtgcaggtc
     2101 ggtggcgtcg gtttccttcg aggtgaggaa cgcgaggata tcgggcaggc ggtcctcgat
     2161 ggcgcggaag ttggggcaat cgcgccggca ctggccgatg tcgaacggcg tctgcccgaa
     2221 attgcgctgg ttcgggtcga cctggcgcag gtcggtcagg tgattcagcc agcacgcctc
     2281 ggcgcaggag ccgatgttga agtacacgcg ctggatcgcc tccagcgcac cgatgctgtc
     2341 ctcaccgcct ttcaggatgt ggtgcaccgc ctcctgcgcg cggcccttgc gccagcactt
     2401 gttctgccgg cccggctcgc accagcaggt gtcctggtcg atttccttct gttcgcaact
     2461 ggccgccttg cgccacttga cgatgtcttc cccgtcgtgc gtgagccgct gcttgatgtt
     2521 gatcagagcg ttgatggtcc cgggattgtt cacctggtcg gtggggatgg ccgaggtgtc
     2581 cgaggtgccg ggccgcgcgt gcgcgaagat ctgccactcc gggctgtcgg cgggcatgcc
     2641 cgacaccatc agctcggaga tgcgcaggta ctggttgccg atgccgccct tgaggttgcg
     2701 ccactgcggg cgctcggggt ccttcggtgg atcgagcgga tcgaaggcga tgtggcacga
     2761 gccgcatgag gttccgatca ggaacggcgg ctcgaccgca ccgtcggaca gccggctggt
     2821 gcgcccgtcg gtggtattcg ggtcggacga caggcgccgg ttgaagcctt cccaggtgcc
     2881 gagcttgccg ccgttcagca tgcgccactg ctccgcgtcg aagcgcgggt tgggaaactt
     2941 gcggaagccg agcgcgccgg tcgaggtgcc gaaggcgaga tcgcaagacg agtggcgctg
     3001 gtcgccgggc ccgtgcacgt ccccctcggc gagcggcggg tctttcatct cgcacgccgg
     3061 atcgcgatag ccctccttgc ccacgtactg caggagctcg gcgtcgccga gacaataatc
     3121 gaagccgtag gtctcctcca ggctcttcgc cggacacccg gcggagcccg gcgtgcagca
     3181 gtccggatcg ttgatcagcc cccaggtacg gaaccgctgg ccgcgctggt cgctgcgcag
     3241 cacccggtac cagtcgatca gcacgcccat gcgctgctgg aacacgtagg tgtggaagcg
     3301 cgcgttgccc gcggtggcct tgtaccagat ctcgcggccg gcgatctcgg aggggctgag
     3361 cgccgcttcg cccaggcgga agcccgcgtc cgcgtagatc gcttcggtgc cgctcttcgc
     3421 cgcaggctca ggcgccgcgg gcgattgcgc gacggcttgt gggaccgcta tccaatccgc
     3481 cggccaatcc gccggtgggt cagcgatgac ccaccatgcc gcggccagaa ccacgatgac
     3541 aagccccaag aaccctgctg tcctcatgcc tgcatcctcc ctgcgagcga accttgtgcg
     3601 gcgccatggc cagatgcaga gcccggccct gcaagaaggc cgggcgcgcg tgccattcga
     3661 cgcacgccat ccgatagcgc aaggctaagg aaaaattcgc caaagggaaa gcccatcgtt
     3721 gcgcgatcgg cagcgcgtct ctgcacaacg acatgcgcta ttgtgcccga cgagggcgcg
     3781 acgcggtttg tgtccgtgcg cagtttcgtg caccatcttc acccgccctc accagcatcg
     3841 ggagcgatca catggctcgg aagaagacga cgcgcgagcc ggcgccacgg cgcaagcgca
     3901 gcacggcagc ggccgcagcg ccggtgccgc cctccgacgc ggccagcgcc ttcgtcatgg
     3961 acaacgacga gatcgaacgg gcgttacgca ccggccagga cgcgggcgcg ctggaagact
     4021 atttcggccc gcgcgactac gccgagctgc gcgagctggc gcagcagagt gcggccaagc
     4081 gcgccgcggc cggaccgaag gtgctgatcc tgcccggcat caccggctcg cgtctcggcc
     4141 agatcgccgt gaacggtcag gagaacatcg tctggttcga tccggtggag ctcgcgttcg
     4201 gcgccatccg caagctcgcc gtgggctcgg gcgtcaagat cgtgccgctg ggcgtgatcc
     4261 tgctgtacta cctgaagctc aagctgcgcc tgcgcgcgct cggcttcgac gccgacttct
     4321 acccctacga ctggcggcag agcctggaca cgctcggcgc gcagctcgcc gagcgcatcc
     4381 gcaacgctcc ggcgctcgac ggcgtgagcc tggtgtgcca cagcatgggc gggctggtcg
     4441 cgcgcgcggc gctggcggcg ctcggcgcgg cgggcaagaa gatcggccgc gtggtgcagc
     4501 tgggcacacc caacttcggc tcgttctgcg cggtgcagac catgcgcgcg cagggcgaca
     4561 cggtgaagaa gctggccttc ctcgatcagc gccacgatgc gcaggagatc gttgccgacg
     4621 tgctggcgcc gatgccgggc ttctgccagc tgctgccctc gcccgagcgc ttcaacgcca
     4681 tcgacctgtt cgacgccagg cgctggccgg ccagccggcc gctgccgcag gccgaggtgc
     4741 tgcgccaggt gccgggcgtg ctcaagaggc tggccccggc cgacgagcgc ttcaccttga
     4801 tcgccgggat caaccgcgag acggtgaccg ggctgcgcaa ggacgagcag aaggacgagt
     4861 tcgtgttcca actgtcgatg gcgggcgacg gcaccgtgcc gctggcgctg gccgagctgc
     4921 ccggcgtgaa ggccacctat tacatcgagg aaacccacgg cagcctgccc aacaacaaga
     4981 tcgtcgcgcg cgccgtcgcc gacatcctga gcaccggcgc gaccgccctg ctgcccagca
     5041 ccgtcggtgc ggtgcggcgc gaggtcacgc gcgaagtgtc cgagagcgcg ctcgccacgc
     5101 agcagcctta ccccggccgc cggcagggcc ggctcagcca gcgcgagctg cgcgaggtgc
     5161 tgtccgaatt cgtttccacc gagacgcgcg acgccgagca accgctggcc atgacggcgg
     5221 ccggggccgc agccccggag gccgacctgg cgcagcgctt cgaccgcctg gtgatcggac
     5281 gccggcggca gcaccgcatg gacctgcggc tggcgctggg cgatgtcggc gacgtgaata
     5341 cgcaggcgct ggtgatcggc atctaccgct cggtcgcgcc cagcggggcg gcgaaggcga
     5401 tcgacgcgag gctcgacgga gcgatcaccg agctcacgca gcggcgcatg ttctccggca
     5461 acgtcggcga ggtattcctg ctgcccaccg cgcagcgcgc ggtgcgcgcc gacatggtgg
     5521 ggttcatcgg tttgggcgcg ttcgacacct tcaccgagga ggtgctgcgc atcgccgccg
     5581 agaacgcgat ccgcacgctg gtgctcggcg gggtgtacga cctcgccacc gtgctcttcg
     5641 gcggcggctc gggcgccggc gtgcgctccg ccgtggaaaa cctgctgtcc ggtttcgtga
     5701 aggggctgat ggatgccgac gccggccacc gtttccgctg cctgacggtg tgcgaaggca
     5761 acccggagcg ctacgaggag atcaagcgcg agctgttccg gctgtcgagc acgccgctgt
     5821 tcgacgagct ggaggtgacg ttcttcgagg agcggctgcc gccggcggtg gcgcccgccg
     5881 gccgtcgcgc agcggcgcgg cccgatccgg tgtacctgct ggttcggcag gaaggcaaga
     5941 ccggcagcgg cctcgagctg cgcacctcgg tgctgggtgc cgggggcaag gcgacggtgg
     6001 tgagcggcgt gcgccagctc aagcgcatcg acctggacgg caagctggac gagctgcaga
     6061 ccagcggctt ccgcttcgat cgcgccccgg cctatggccg cgagctctcg aacctggtgg
     6121 tctccgacga ggcgctgacg gcgctcgccg ctctgaagga tcggcacgtg gtggtggtgc
     6181 acgacgcgga atcctcgcgc atcccctggg agctgctggc tgtgcccggc agcgcacagg
     6241 ccactacgct gctcgcgaag gagggcggct tgagccggcg ctacgtcgcc gacaacctgt
     6301 cggtggcgaa gtggctggaa gagcgccgcc aggatctcga gctcaacctg ctgctggtgg
     6361 tgaacccgac gctggacctc gacggcgcgg aggaggaagg ccgccgcatc cgcgagctgc
     6421 tggcgggcga gccggcggtg cgcgtcgacg agctgcgcgg ccgcgacgcg agccgcccgg
     6481 cgctcgagcg cgcgttccgc tcgggcaagt acgacgtgat ccattacgcc ggccacgcgt
     6541 tcttcgacga agccgacccg gcggcgagcg gcatcctgtg cgccggcaag gcggtgttga
     6601 gcggacgcga cctggcgggg ctgggaaatc tgcccgcgct ggtgttcttc aatgcgtgcg
     6661 aagccggacg cgtgcggcgc gccgggcgcg cagcacaagc ggctccgagc atgcgcgagc
     6721 gcatcgatcg caacgtcggc ctggccgaag ccttcctgcg cggcggcgtg gccaactacg
     6781 tgggcaccta ctggccggtg ggcgacgagc cggccaagct gttcgccgag cagttctacc
     6841 gctcgctgat gcaggggcag agcatcggcg atgccttgct cgcggggcgc cgcaccgtgt
     6901 gggagcgcaa gtcggtggac tgggccgact acattcacta cggcagccac gatttcgtcc
     6961 tcaagacgcg gtgaagccgc gccgacgagg tcccggcgca cccgctggtc tgcgcagcgt
     7021 gcgcgtccgc gccgcggaac ccatggtctt gaccggcggc ggttcaggta aactccgtta
     7081 cgcgaccagc ccggtcggtg cggcattccc atccgcggtc gcccgcgatt cggacgggcc
     7141 ggcgcaacga aaccgacatt cgcggttcat cgccgcttaa ctgcatctac gtatcttcca
     7201 gcatggcaga ttcgtgccga tcccggcctc cgcgcattcc atggcgctgg ggcttccgaa
     7261 tcccactgga ggaatcatcg atgcagcgtg agaactcgat ctcagtgagc gcaccaggtg
     7321 ttcgggtgcg ttcgcgccgc tcttttctga agggctccgg cctgctcgca ggcggaatca
     7381 tcacctcggc tacactcgcc gggctcgccg cgcacagcgc gcaggcgcac gccggcgagg
     7441 accgcgatca ccgcgagtgc cgccgcgcac gcggccggcg cagcgactac ggtaagctca
     7501 cccccacgcc ggaccaggac ggggcgcaca tcctggcgct gccgcgcggg tttcactacg
     7561 tgactttcag caagaccggc gaggttttcg gcgacggcct gctggtgccg cgaaaccac
//
LOCUS       DASPGE010000180        10624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_49232, whole genome shotgun sequence.
ACCESSION   DASPGE010000180 DASPGE010000000
VERSION     DASPGE010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10624
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49232"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..1477)
                     /locus_tag="VD791_05735"
     CDS             complement(302..1477)
                     /locus_tag="VD791_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HXV07509.1"
                     /translation="MTVRSALSKRLDTELQLDTHPETRLDFDQVRLAARQGYDRPFLL
                     VDPQIIRIKARRFTAAMPRVHPHYAVKANPHPQVLRTLIEEGTSFEIASVAELDLLLG
                     LGVPAHEIFYSNPVKSPRYVEHAAARGVRWFVLDSVEELRKITAIAPGASLYLRIQVS
                     NAGSDWPLSGKFGAQPAEVDEIIAAAAERKADLAGVTFHVGSQCRNADNWRAGIEGAK
                     RVFRKMRLKGLRPRLLNIGGGYPVRHTKPIPSIEVIGEVVNRALEGLPGEVRVMAEPG
                     RFLVSDSGWFVCRVVGIATRGGKRWAYLDAGMFGGIIETTEGLQYDVVTERTGRLVPW
                     TVAGPTCDSVDIVMRDQLLPEDLGADDFVYIPNGGAYTTAYASNFNGFPLPEVRILG"
     gene            complement(1788..2360)
                     /gene="dcd"
                     /locus_tag="VD791_05740"
     CDS             complement(1788..2360)
                     /gene="dcd"
                     /locus_tag="VD791_05740"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HXV07510.1"
                     /translation="MSIKSDRWIRRMATQHRMIEPFEPGQVKQLDGRPIVSYGTSSYG
                     YDVRCSDEFKLFTDINTTIVDPKSFDPKSFVDVKGEFCIIPPNSFALARTVEYFRIPR
                     NVLVVCLGKSTYSRCGIIVNVTPLEPEWEGHVTLEFSNTTPLPAKIYANEGVAQVIFL
                     EAAADDVCETSYKDRGGKYQGQSGVTLPKI"
     gene            complement(2449..3540)
                     /gene="apbC"
                     /locus_tag="VD791_05745"
     CDS             complement(2449..3540)
                     /gene="apbC"
                     /locus_tag="VD791_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HXV07511.1"
                     /translation="MPITEAQVQEALKATIDPTTGKDYAAAKAVKAIKVDGGGIAVDI
                     VLGYPAKSVLERVREQIRAALQALPGAGAVEVNVSFKITSHAVQGGVERVPGVRNIVA
                     VASGKGGVGKSTVAVNLALALAAEGARVGILDADIYGPSQPLMLGLQGHKPVSPDRTS
                     MEPLEAHGIQAMSIGFLIDVDQPMVWRGPMVTQALEQLLRFTRWRDLDFLVVDMPPGT
                     GDIQLTLAQKVPVTGAVIVTTPQDIALIDARKGLKMFEKVNIPILGVVENMALHVCPA
                     CGHEEHIFGAGGGQKMATDHGVTLLGSLPLDIRIREQADSGNPTVVADSAGRTAEIYR
                     EIARKLAVRIAEKAQDYSAAFPKIVIKNT"
     gene            3614..5698
                     /gene="metG"
                     /locus_tag="VD791_05750"
     CDS             3614..5698
                     /gene="metG"
                     /locus_tag="VD791_05750"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HXV07512.1"
                     /translation="MRRQILVTSALPYANGSIHIGHLVEYIQTDVWVRFQKMRGHECH
                     YVCADDTHGTPIMLRAEKEGIAPEALIERVWKEHTRDFAGFHVGFDNYHTTHSEETRA
                     LSEDIYLRLKERGLIEVRSVEQFYDPVKEMFLPDRFIKGECPNCHAKDQYGDSCEVCG
                     TTYSPTDLIGAYSAVSGAAPVRKASDHHFFRLSHPDCREFLEQWTQSSEHLQEEARNK
                     IREWFDQGLNDWDISRDAPYFGFQIPGAPGKYFYVWLDAPIGYLGSFRHLAQRLKLDF
                     AAWVRPGSAAEMVHFIGKDILYFHALFWPAMLKFGGYRTPTRVFAHGFLTVDGQKMSK
                     SRGTFITAESYLAQGLNPEWLRYYYAAKLGPAMEDIDLNFEDFVARVNSDLVGKYVNI
                     ASRAAGFVHKRFGGRLIEPERRASGAAERPDLLLYASENDIAEAYERREYSKAVREIM
                     RLADLINQYADRHKPWELARQPEQSERLHQVCSELLNAFRVLTIYLAPILPATAQRVA
                     AFMNLQGKLDWEHLAEYPRAPIDEYRHLMTRVEATQVQALVAANKESLQPSAAAHSPQ
                     RHAQHQEQSMETPVGPPAASSSISIDDFNKVELRVARIVDAQHVEGADKLLRLQLDLG
                     GETRQVFAGIKSAYDPGELKGRLTVMVANLQPRKMRFGESQGMVLAASGEGPGIFLLS
                     PDDGAQPGMRVK"
     gene            5739..6005
                     /locus_tag="VD791_05755"
     CDS             5739..6005
                     /locus_tag="VD791_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HXV07513.1"
                     /translation="MKITKHVLVHGRVQGVNFREATRLQAQRCNVGGWVRNRDDGSVE
                     AVLHGVPDDVARMLDWMHRGPPAARVVRIEVDDWHGEFEDFERR"
     gene            complement(6011..7336)
                     /locus_tag="VD791_05760"
     CDS             complement(6011..7336)
                     /locus_tag="VD791_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXV07514.1"
                     /translation="MSAGPGRRTPGHRAVLGIAGPIMISNVSTPLLGVVDTAVVGQAP
                     DPAYIGAVALGALVFNFVFWGFGCLRMGTTGLTAQALGAGDAAELRASLGRAALIAAA
                     VGAGLIALQWPIREAAFALLEGSPRVEDLARGYFDLRIWAAPATLLNYALLGWFIGLG
                     RARIALALQLLLNFSNMALDAFFVLVLDMGVHGVALGTVLAETAAAAAGLSVAARHLR
                     RFGGRWDRERLLAGDRLRRMIAVNGDIMVRSAALILAFVWFMAQGAKQGDEILAANAV
                     LMHFIAVAAFFLDGLAFAAEALVGRAVGAAERAGLLAAARITTWWAAGVALLASIVFA
                     LLGPGIVDLLAVDEGVRATARAYLPWAAFGPLVGVWCFQLDGIFIGATRTAQMRNAGL
                     ASLAIFLAAWWALQPFGNHGLWAALYVHYAARALTLLVCFPALVRAVPR"
     gene            complement(7333..>10624)
                     /gene="dnaE"
                     /locus_tag="VD791_05765"
     CDS             complement(7333..>10624)
                     /gene="dnaE"
                     /locus_tag="VD791_05765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HXV07515.1"
                     /translation="CDLWLTNEQDRDKPFRVLVLCQNRAGYHTLCELLSRAFRSNQHR
                     GRAQVKKSWFLELGAEGLIGLSGAAAGDVGQALLSDNESGAELAARSWAQLFPGRFYI
                     ELQRSGAPDQEAYVARAARLASRLRLPVVATHPVQFLTPDDFKAHEARVCISQGYILA
                     DQRRPVRFTAEQYFKSQAQMAQLFADLPAALANSVEIARRCSLEIELGAAKLPLFPTP
                     GNTGLDDYLRSQAVEGLARRLAVLYPDARQREAQAPRYRERLETEIGTIIEMGFPGYF
                     LIVADFINWAKRNGVPVGPGRGSGAGSLVAWCLGITDLDPLRYGLLFERFLNPERVSM
                     PDFDVDFCQDGRDRVIEYVRQKYGAQSVSQIATFGTMAAKAVVRDVGRVLDMPYNFCD
                     QLAKLIPFQPGKLITLQMAREMEPLLAAREREEEEVRELLALAERLEGLIRNVGMHAG
                     GVLIAPGELTDFCPLYCAEGTDAVVSQFDKDDVEAVGLVKFDFLGLTTLTILDWTLRY
                     VDRLAGAGAAPAAALAPGLKLETLPLDDAATYRIFATANTTAVFQFESRGMRDLLSSA
                     KPDRFEDVIALVALFRPGPMELIPDFIKRKHGRQRVDYLDPRLQPILGETYGIMVYQE
                     QVMQIAQVIGGYTLGAADLLRRAMGKKKPEEMAQQRDIFVAGAQANGLTRGTASQLFD
                     LMEKFAGYGFNKSHAAAYALVAYQTAYMKAHHAAAFLAANMSAVMDDTDKVHQLYGDA
                     LANGLTVLPPDINASEYRFVPVSAQQIRYGLGGIKGTGESAIQAIVAAREADGPFRDL
                     FDFCRRVDKRVVNRRVLEALIRAGAFDGIDDHRHRLFASAGIAMEGAEQVSRAAVQSS
                     LFGADEGAGGFAQALIERPRWSERERLQAEKQALGFYLSGHPYNEYRAELQRFVRTTL
                     AQLQPPGDGDNGQRTQLIAGIVESLRWANTQSGRMLIVRLSDGSATREVTVYNELADQ
                     SRGLIKEDALLVVEARVRTVRRALDEGEETFMRVSADKLYDLASARSRFARAIKLTCN
                     GGSSGARLRELLGAHKPGACPVSILYRNGDAQCRIDLGEAWRVKLDDTLIASLHDWLQ
                     PENVEIVY"
BASE COUNT         1691 a         3691 c         3603 g         1639 t
ORIGIN      
        1 gtggctcagc ggcgcggcgc agagcagcag cgcagcgcag gccggcagca ggccccatcg
       61 gttgcctcgt cccataactc ccccctgcgg ctgccggcag aggaaccggt agggaggttc
      121 gcacctgcgc cgcgcggaac cggccacttc ttgaagcagg aatcgaagat ttatggttta
      181 tcgcttgaaa actaacgtat ctcctatgcg cggtcaatgt gaccggcggg cgacacgagc
      241 gcgatgaagc ggacgcgcgc tgcgcgcgcc cgcttccggg ggagagcagc ggcggcagtc
      301 cctaaccgag gatgcgcacc tcgggcagcg gaaagccgtt gaagttgctg gcgtacgcag
      361 tggtgtacgc gccgccgttg ggaatgtaga cgaagtcgtc ggcgcccagg tcttccggca
      421 gcagctggtc gcgcatcacg atgtccaccg aatcgcaggt cgggcccgcc accgtccagg
      481 ggacgagccg gccggtgcgc tcggtcacca cgtcgtactg cagaccctcg gtggtctcga
      541 tgatgccgcc gaacatgccg gcgtcgaggt acgcccagcg tttgccgccg cgcgtggcga
      601 taccaaccac ccggcagacg aaccagccgg aatcggacac caggaagcgg cccggctcgg
      661 ccatcacccg gacctcgccc ggtagcccct ccagcgcgcg gttcaccacc tcgccgatga
      721 cctcgatcga cgggatcggc ttggtgtggc gcaccggata gccgccgccg atgttgagca
      781 agcgcgggcg caatcccttc agccgcatct tgcggaacac gcgcttcgcg ccctcgatcc
      841 ccgcccgcca gttgtccgcg ttgcggcact gcgagccgac gtggaacgtc accccggcga
      901 ggtcggcctt gcgctccgcc gcggcggcga tgatctcgtc cacctcggcc ggctgcgcgc
      961 cgaacttgcc cgacaacggc cagtcgctgc cggcgttgct gacctggatg cgcaggtaca
     1021 ggctggcgcc gggggcgatc gcggtgatct tgcgcagctc ctcgacgctg tccagcacga
     1081 accagcgcac cccccgggcc gccgcgtgct cgacgtagcg cggcgacttc accgggttgc
     1141 tgtagaagat ctcgtgcgca ggcacgccca ggccgagcag gaggtcgagc tcggcgacgg
     1201 aggcgatctc gaagctggtc ccctcctcga tcagcgtgcg cagaacctgc gggtgcgggt
     1261 tggccttcac cgcgtaatgc ggatgcacgc gcggcatcgc ggcggtgaag cgccgtgcct
     1321 tgatgcggat gatctgcgga tcgaccagca ggaacggcct gtcgtagccc tgcctggcgg
     1381 ctaggcgtac ctggtcgaag tcgaggcggg tttcggggtg ggtgtcgagc tggagctcgg
     1441 tatcgaggcg cttggagagc gcgctgcgga cggtcatcga cttctcctcg aggacgctag
     1501 gtcagtggga tgtgcaggtt ggtcagatcc acgctgggga agtaattcct ttcgactgtc
     1561 atgatgacct cctgggcgcc atgaagaact ggagaggggc aattataggg acaacccccc
     1621 gggaatcaag aggaaatttg gcctccggga gctccggtgg ccggccgggc cggccgggcc
     1681 gaaaagcgta accgaagggc gtgacgaaga ccgtcggcca tccagcgggc ctggttttgt
     1741 cacgcttccc ggtcacgcag taccggtcac gccttttcgg tcagccccta gatcttcggc
     1801 agcgtcacgc cgctctggcc ctggtacttg ccgccgcggt ccttgtagga cgtctcgcaa
     1861 acgtcgtccg ccgcggcttc gaggaagatc acctgcgcca cgccctcgtt ggcgtagatc
     1921 ttggcgggca gcggcgtggt gttggagaac tcgagcgtga cgtggccctc ccactcgggc
     1981 tcgaggggcg ttacgttcac gattattccg catctcgaat atgtcgattt tcccaggcat
     2041 accaccagca cgttgcgcgg gatgcggaag tactcgacgg tacgcgccag cgcgaaggag
     2101 ttgggcggga tgatgcagaa ctcgcccttg acgtcgacga aggacttggg gtcgaagctc
     2161 ttggggtcga cgattgtcgt gttgatgtcg gtgaacagct tgaactcgtc cgagcagcgc
     2221 acatcgtagc cgtagctcga agtgccgtaa gacacgatcg gccgcccgtc gagctgcttc
     2281 acctggcccg gctcgaacgg ctcgatcatg cggtgctgtg tcgccatgcg gcggatccag
     2341 cggtcggatt tgatgctcat ctctctcctg ccttgaccgt aacggcgcag ctagcccgtg
     2401 cgcccccgcc cctctgggtt gccgagcgcg cctcagccgt tgcgcttctc acgtgttctt
     2461 gatgacgatc ttggggaacg cggcgctgta gtcctgcgcc ttctccgcga tcctcaccgc
     2521 cagcttgcgc gcgatctcgc ggtagatctc cgcggtccgg ccggccgagt cggcgaccac
     2581 cgtcggattg cccgagtctg cctgctcgcg gatgcggatg tcgagcggca gcgagccgag
     2641 cagggtcacg ccgtggtcgg tggccatctt ctgcccgccg ccggcgccga agatgtgctc
     2701 ctcgtggccg caggccgggc acacgtgcag cgccatgttc tccaccacgc cgaggatcgg
     2761 gatgttcacc ttctcgaaca tcttcagccc cttgcgcgcg tcgatcaggg cgatgtcctg
     2821 gggcgtggtg acgatcaccg cgccggtcac cgggaccttc tgcgcgagcg tgagctggat
     2881 gtcgccggtg ccgggaggca tgtccaccac caggaaatcc aggtcgcgcc agcgcgtgaa
     2941 gcgcagcagc tgctccagcg cctgcgtgac catcgggccg cgccagacca tcggttgatc
     3001 cacgtcgatg aggaaaccga tcgacatcgc ctggatgccg tgcgcttcca gcggctccat
     3061 cgaagtgcga tccgggctca ccggcttatg tccctgcagg cccagcatca acggttgcga
     3121 cgggccgtag atgtctgcgt cgaggatacc gacgcgggcg ccctcggccg caagcgccag
     3181 cgccagattg accgctaccg tggacttgcc aacgccgccc ttgcccgagg ccacggccac
     3241 gatgttgcgc acgccgggca cgcgctccac cccgccctgc accgcgtgcg aggtgatctt
     3301 gaagctgacg ttgacctcca ccgcgcccgc tccggggagc gcctgcagcg ccgcgcggat
     3361 ctgctcgcgc acccgctcca gcacgctctt cgccggatag ccgagcacga tgtccaccgc
     3421 gatgccgccg ccgtccacct tgatcgcctt caccgccttg gcggcggcgt aatccttgcc
     3481 ggtcgtgggg tcgatggtcg ccttcagcgc ttcctggact tgtgcttcgg tgatcggcat
     3541 ggtcggtctc gcgtgggccc ggattctact gcttttcaac cctgttagac tggtggtttt
     3601 cgctttcgtc acgatgcgcc ggcagattct cgtcacctcc gcgctgccct atgccaacgg
     3661 cagcatccac atcggccacc tggtggagta catccagacc gacgtgtggg tgcgcttcca
     3721 gaagatgcgc gggcacgagt gccattacgt ctgcgccgac gacacgcacg gcacccccat
     3781 catgctgcgc gccgagaaag agggcatcgc gccggaagcc ctgatcgagc gcgtgtggaa
     3841 ggagcacact cgcgacttcg ccggcttcca cgtcggtttc gacaactatc acaccacgca
     3901 ttcggaggag acgcgcgcgc tgtccgagga catctacctg cggctcaagg agcgcgggtt
     3961 gatcgaggtg cgctcggtgg agcagttcta cgatccggtg aaggagatgt tcctgccgga
     4021 ccggttcatc aagggcgagt gccccaactg ccacgccaag gatcagtacg gcgactcctg
     4081 cgaggtgtgc ggcaccactt acagcccgac cgacctgatc ggcgcctact ccgcggtctc
     4141 cggagcggcg ccggtgcgca aggcttccga tcaccacttc ttccggctgt cgcacccgga
     4201 ctgccgcgag ttcctggagc agtggacaca gtcgagcgag cacctgcagg aggaggcgcg
     4261 caacaagatc cgcgagtggt tcgaccaggg tctgaacgac tgggacatct cgcgcgacgc
     4321 gccctatttc ggcttccaga tccccggcgc gccgggcaag tacttctacg tgtggctgga
     4381 cgccccgatc ggatacctgg gcagcttccg ccacctggcg cagcgcctca agctcgactt
     4441 cgccgcctgg gtgcgtccgg gttccgccgc cgagatggtg cacttcatcg gcaaggacat
     4501 cctctacttc cacgcgctgt tctggccggc gatgctcaag ttcggcggct accgcacgcc
     4561 cacgcgcgtg ttcgcgcacg gtttcctcac cgtggacggg cagaagatgt cgaagtcgcg
     4621 cggcaccttc atcaccgccg agagctacct ggcgcagggc ctcaacccgg aatggctgcg
     4681 ctactactac gcggccaagc tcgggcccgc catggaggac atcgacctca acttcgagga
     4741 cttcgtggcg cgcgtgaaca gcgacctggt gggcaaatac gtgaacatcg ccagccgcgc
     4801 ggcgggtttc gtgcacaagc gcttcggcgg gcggctgatc gagccggaac ggcgcgcttc
     4861 cggcgcggcc gagcgccccg acctgctgct gtacgcctcg gagaacgaca tcgccgaggc
     4921 ctacgagcgc cgcgagtaca gcaaggcggt gcgcgagatc atgcggctcg ccgacctgat
     4981 caaccagtac gccgaccggc acaagccctg ggagctcgcc cggcagccgg agcagtccga
     5041 gcgcctgcac caggtgtgca gcgagctgct caacgcgttc cgcgtgctca ccatctactt
     5101 ggcgccgatc ctgcccgcca ccgcgcagcg cgtggccgcc ttcatgaacc tgcagggcaa
     5161 gctcgactgg gagcacctgg ccgagtaccc gcgcgcgccg atcgacgagt accggcacct
     5221 catgacgcgg gtggaggcga cgcaggtcca ggctttggtc gcggccaaca aggaaagcct
     5281 gcagccgtcc gcagccgccc attcgcccca acgccatgcc caacaccagg agcagtccat
     5341 ggaaaccccg gtgggacccc cggcagcctc gtccagtatc tcgatcgacg acttcaacaa
     5401 ggtcgagctg cgcgtcgcgc gcatcgtcga cgcgcagcac gtcgagggcg ccgacaagct
     5461 gctgcgcctg cagctcgacc tgggcggcga gacccggcag gtgttcgccg ggatcaagtc
     5521 ggcctacgat ccgggcgagc tcaagggacg gctcacggtg atggtggcca atctgcagcc
     5581 gcgcaagatg cgcttcggcg aatcccaggg catggtgctc gcggccagcg gagagggacc
     5641 cggcatcttc ctgctgtcgc ccgacgacgg cgcgcagccg ggcatgcggg tgaagtagtt
     5701 cccctctccc ctcgcgggag agggttgggg tgagggggat gaagatcacc aagcacgtcc
     5761 tcgtgcacgg ccgcgtgcag ggcgtcaact tccgcgaagc gacccgcctg caggcgcagc
     5821 gctgcaacgt cggcggctgg gtgcgcaacc gcgacgacgg cagcgtcgag gcggtgttgc
     5881 acggcgtccc ggacgacgtg gcgcgcatgc tcgactggat gcaccgcggc ccgcccgccg
     5941 cgcgcgtcgt gcgcatcgag gtggacgact ggcacggcga gttcgaggac ttcgagcggc
     6001 gctagcctga ctaccgcggt accgcgcgca ccagcgcggg aaagcaaacc agcagcgtta
     6061 gggcgcgggc cgcgtagtgc acgtagaggg cggcccacag accgtgattg ccgaaaggct
     6121 gcagcgccca ccaggcggcc aggaagatgg cgagcgaggc gagcccggca ttgcgcatct
     6181 gcgcggtgcg cgtcgcgccg atgaagatgc cgtcgagctg gaagcaccac acgcccacca
     6241 gcggtccgaa cgccgcccag ggcagatagg cgcgcgcggt cgcgcgcacg ccctcgtcca
     6301 ccgcaagcag gtcgacgatc cccgggccga gcagcgcgaa gacgatcgag gccagcagcg
     6361 cgacgcctgc ggcccaccag gtcgtgatgc gggcggccgc cagcagcccc gcgcgctcgg
     6421 ccgcgcccac cgctctgccg accagggcct cggccgcgaa cgcgagcccg tccaggaaga
     6481 acgcggcgac ggcgatgaag tgcatcagca ccgcattcgc ggccaggatc tcgtcgccct
     6541 gcttcgcccc ctgggccatg aaccatacga aggcgaggat cagcgcagcc gagcgcacca
     6601 tgatgtcgcc gttcacggcg atcatgcgcc gcagccgatc gccggccagc agccgctcgc
     6661 ggtcccagcg cccgccgaac cggcgcaggt gccgcgcggc gaccgacagg cccgcggccg
     6721 ccgccgcggt ctccgccagc accgtgccga gcgccacgcc gtgcacgccc atgtccagga
     6781 ccagcacgaa gaacgcgtcc agcgccatgt tgctgaaatt gagcaggagc tgcagcgcca
     6841 gcgcgatgcg cgcgcggccc aggccgatga accagccgag cagcgcgtag ttgagcaggg
     6901 tcgcgggcgc cgcccagatg cgcaggtcga agtagccgcg cgccaggtcc tcgacccggg
     6961 ggctgccctc gagcagcgcg aaggcggctt cgcggatcgg ccactgcagg gcgatgagcc
     7021 cggctccgac cgcggcggcg atcagggccg cgcggcccag gctggcgcgc aactccgcgg
     7081 catcgcccgc gccgagcgcc tgcgccgtca ggccggtggt gcccatgcgc aggcaaccga
     7141 agccccagaa cacgaaattg aacaccagcg cgccgagggc caccgcgccg atgtacgccg
     7201 gatcgggcgc ctggccgacc acggcggtat cgaccacgcc gagcagcggc gtggacacgt
     7261 tcgagatcat gatcggcccg gcgatcccca ggaccgcgcg atggcccggg gtccggcgcc
     7321 cggggccggc gctcaataca cgatctcgac gttttccggc tgcagccagt cgtgcagcga
     7381 cgcgatcagc gtgtcgtcca gcttcacccg ccacgcttcg cccagatcga tgcggcactg
     7441 cgcatcgccg ttgcggtaca ggatcgacac cgggcaggcg cccggcttgt gcgccccgag
     7501 cagctcgcgc aggcgcgcgc cgctggaccc gccgttgcag gtgagcttaa tggcgcgcgc
     7561 gaacctgctg cgcgccgagg ccagatcgta gagcttgtcg gcgctgacgc gcatgaacgt
     7621 ctcctcgccc tcgtcgagcg cgcggcgcac ggtgcgcacg cgcgcctcga ccaccagcag
     7681 cgcgtcttcc ttgatcaggc cgcgggactg gtccgccagc tcgttgtaga cggtcacctc
     7741 ccgggtggcg ctgccgtcgc tcagccgcac gatcagcatc cgccccgact gcgtgttcgc
     7801 ccagcgcagc gactcgacga tgccggcgat cagttgcgtg cgctggccgt tgtcgccgtc
     7861 gccgggcggc tgcagctggg cgagcgtggt gcgcacgaag cgctgcagct cggcgcggta
     7921 ctcgttgtag ggatgtccgc tcaggtagaa cccgagcgcc tgcttctccg cttgcagccg
     7981 ttcgcgctcg gaccagcgcg ggcgctcgat cagcgcttgc gcgaacccgc cagcgccttc
     8041 gtccgcgccg aacagggagc tctgcaccgc cgcgcggctg acctgctcgg cgccctccat
     8101 ggcgatgccg gcggaggcga acagccgatg gcggtgatcg tcgatgccgt cgaacgcgcc
     8161 ggcgcggatc agcgcctcca gcacgcgccg gttcaccacg cgcttgtcca cgcggcggca
     8221 gaaatcgaac aggtcgcgga acgggccatc cgcctcgcgc gcggcgacga tcgcctggat
     8281 cgccgactcg ccggtgccct tgatgccgcc caggccgtag cggatctgct gcgcgctcac
     8341 cggaacgaag cggtactcgg acgcgttgat gtcgggcggc agcacggtga gcccgttggc
     8401 cagcgcgtcc ccgtagagct gatgcacctt gtcggtgtcg tccatcaccg ccgacatgtt
     8461 ggcggccagg aaggcggcgg cgtggtgcgc cttcatgtaa gcggtctggt aggccaccag
     8521 cgcgtaagcc gcggcgtgcg acttgttgaa gccgtagccg gcgaacttct ccatcagatc
     8581 gaacagctgg ctcgccgtgc cgcgggtcag gccgttcgcc tgcgcgccgg ccacgaagat
     8641 gtcgcgctgc tgcgccatct cctcgggttt cttcttgccc atcgcccgac gcagcaggtc
     8701 tgcggcgccc agggtgtagc cgccgatcac ctgcgcgatc tgcatcacct gctcctggta
     8761 caccatgatg ccgtaggtct cgcccaggat cggctgcagg cgcggatcga ggtagtccac
     8821 gcgctggcgc ccgtgcttgc gcttgatgaa atcgggaatc agctccatcg gcccgggccg
     8881 gaacagcgcc accagggcga tgacgtcctc gaagcggtcg ggcttggcgc tgctgagaag
     8941 atcgcgcatg ccgcgcgatt cgaactggaa caccgcggtg gtgttggccg tggcgaatat
     9001 gcggtaggtc gccgcgtcgt ccagcggcaa ggtctccagc ttcaggccgg gggcgagcgc
     9061 tgcggcgggc gccgcgccag cgccggcgag ccggtcgacg tagcgcagcg tccaatcgag
     9121 gattgtgagc gtggtcagcc cgaggaagtc gaatttcacc agcccgaccg cctccacgtc
     9181 gtccttgtcg aactggctca ccacggcgtc ggtgccctcg gcgcagtaca gcgggcagaa
     9241 gtcggtgagc tcgccgggcg cgatcagcac gcccccggcg tgcatgccga cgttgcggat
     9301 caggccctcc aggcgttcgg ccagggccag cagctcgcgc acctcctcct cctcgcgctc
     9361 gcgcgcggcg agcagcggct ccatctcgcg cgccatctgc agcgtgatca gcttgccggg
     9421 ctggaacggg atcagcttgg cgagctggtc gcagaagttg tacggcatgt ccagcacgcg
     9481 accgacgtcg cgcaccaccg ccttggccgc catggtgccg aaggtggcga tctgcgacac
     9541 cgactgcgcg ccgtatttct ggcgcacgta ctcgatcacg cggtcgcgcc cgtcctggca
     9601 gaagtccacg tcgaagtcgg gcatcgagac gcgctccggg ttgaggaagc gctcgaacag
     9661 caggccgtag cgcagcggat cgagatcggt gatgcccagg caccaggcca ccagcgagcc
     9721 ggcgccggac ccgcgccccg ggccgaccgg cacgccgttg cgcttggccc agttgatgaa
     9781 gtcggcgacg atcaggaaat agccagggaa gcccatctcg atgatggtcc cgatctcggt
     9841 ctccagccgc tcgcgatagc gcggcgcctg cgcttcgcgc tgccgcgcat cgggatacag
     9901 caccgccagc ctgcgcgcca ggccttcaac ggcctggctg cgcaggtagt cgtccaggcc
     9961 ggtgttgccg ggcgtgggaa acagcggcag cttggcggcg cccagctcga tctccaggct
    10021 gcagcgccgc gcgatctcga cgctgttggc gagcgccgcc ggaagatcgg cgaacagctg
    10081 cgccatctgc gcctggctct tgaaatactg ctccgcggtg aaccggaccg ggcgccgctg
    10141 gtcggccagg atgtagccct ggctgatgca cacgcgcgcc tcgtgcgcct tgaagtcgtc
    10201 gggcgtcagg aactgcaccg ggtgcgtggc caccaccggc agccgcaagc gcgacgccag
    10261 gcgcgccgcg cgcgccacgt acgcctcctg atcgggcgca ccgctgcgct gcagctcgat
    10321 gtagaagcgg ccgggaaaca gctgtgccca ggagcgcgcg gcgagctcgg cgcccgactc
    10381 gttgtcggac aggagcgcct ggccgacgtc gcccgcggca gcgcccgaca atccgatcaa
    10441 cccttcggcg ccgagctcga ggaaccagga tttcttcacc tgcgcgcgcc cgcggtgctg
    10501 gttcgagcgg aaagcgcgcg acagcagctc gcacagcgta tggtagccgg cgcggttctg
    10561 gcacagcacc agcacgcgga acggcttgtc gcggtcctgc tcgttggtga gccacaggtc
    10621 gcag
//
LOCUS       DASPGE010000181         4302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_166072, whole genome shotgun sequence.
ACCESSION   DASPGE010000181 DASPGE010000000
VERSION     DASPGE010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166072"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VD791_05770"
     CDS             complement(<1..361)
                     /locus_tag="VD791_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXV07516.1"
                     /translation="MSTRHHRLVILGSGPAGYTAAVYAARANLKPVLITGLAQGGQLM
                     TTTDVDNWPADAGGVQGPELMERFQKHAERFETELVFDQVNKVELAGRPFVLHGDSGT
                     YTCDALIIATGASAKYLG"
     gene            415..2103
                     /locus_tag="VD791_05775"
     CDS             415..2103
                     /locus_tag="VD791_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HXV07517.1"
                     /translation="MARRWGALGALCVAMAAAAPAAAQGDAAPRFEIRRFVIDGATLV
                     PQARLREATAPFTGAGRDFGDVQRALEAVERLYAEAGYSAVQVLLPEQELERGEVRLR
                     VLEARLGRVLVEGNRHFDEANIRASVPALVPGQAPNILQVGRNLRVANESPAKQATVL
                     LRSGGEETTVDAVVRVTDEPPTKVSVTLDNTGTSETGRLRFGLGLQHANLAGGDQVLT
                     LQYVGSPHDEDDTGNLALEPSERVLIVGVGLRIPLYARGDALDFTAGYSNVDSGTVGG
                     LYNVSGAGGLFSARYTHYLDRAGDYDQRVALSWDHRAYSFKDVRAIGSNEQLQPDITV
                     RPLSLAYHGLLRRPDSETAVSVGVSRNLPGGNDGSPEDFCQLPPNAPHGVSRSDGMGN
                     CPDPYYVLWRWSINHNHSLAGDWQMRFGLGGQYTRDMLVSGEMFGLGGADSVRGFDER
                     AIADDIGYRGTLELYSPDFGAWAGWTGARARALVFYDWGALRRNQPAPGDVVAQSIAS
                     VGVGLRITHGTRLSLRADWGWVVDEGGVPGLGTDPGTYKATGEGRLHASVAYLF"
     gene            2153..2821
                     /locus_tag="VD791_05780"
     CDS             2153..2821
                     /locus_tag="VD791_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXV07518.1"
                     /translation="MATTVSPAVLRTVPLFASFSEEQLRALATLVTRRSASRGSIIVA
                     AGDPTDSLYIILSGRFKVMMSDAEGKEVILSILTGGEFFGEMGLIDDAPRSASVVAIE
                     PCELLVLNRRDFKKCMTENAEMAMGVMRGLVRRLREADRKIGSLALLDVYGRVARLLL
                     DMAETVDGQKMVTKRLPKQDIAKMIGASREMVSRVMKDLQTGGYIEMRGSSILLRDTI
                     ALPD"
     gene            2918..3628
                     /locus_tag="VD791_05785"
     CDS             2918..3628
                     /locus_tag="VD791_05785"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HXV07519.1"
                     /translation="MNSLRVLLAAASLLALPAVALAGDGSVAQLSGTLTVKKADGSVR
                     ILSRQSQIEQGDTLTTERDSYAQIRFADGGSLTLKPRTAVRLDQFRFTQDKPREDAFA
                     VRLLKGGLRAVTGGIGKRNPENYEMTTETATVGVRGTTFSVDDCVNARGGECARLAPA
                     VYVSVSDGEVVLRNGAGELALGAGQFGQIVRGERPLFQSTDPGLHFAPPATFIQSILS
                     GRQVAGIGDKLECAISAR"
     gene            3743..>4302
                     /locus_tag="VD791_05790"
     CDS             3743..>4302
                     /locus_tag="VD791_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HXV07520.1"
                     /translation="MLAGLAGYLALIFLTFDRGDPGWSHSAPAATLHNAGGRAGAWIA
                     DLLLYLFGVSAYWWVALCLVGVAWGYRRLERSALIDRRPLAIALGGFALLLLASASLE
                     ALRLHSLAVALPLAPGGMLGDALGGLAARLLGFTGATLLLLTLAAVGFSLFTGVSWLA
                     VAEATGTLLENGAALARRAWEGRADRR"
BASE COUNT          609 a         1515 c         1533 g          645 t
ORIGIN      
        1 ggccgagata cttggccgac gccccggtgg caatgatgag cgcgtcgcat gtgtaggtgc
       61 cggaatcccc gtgcagcacg aacgggcgcc cggcgagctc caccttgttc acctggtcga
      121 acaccagctc ggtctcgaag cgctcggcgt gcttctggaa gcgctccatc agctccgggc
      181 cctgcacgcc gccggcatcc gcgggccagt tgtccacgtc ggtggtggtc atcagctgac
      241 cgccctgcgc cagcccggtg atgagcacgg gcttgagatt ggcgcgcgcg gcgtagaccg
      301 cggccgtata gccggcgggg ccggagccga gaatgacgag gcgatgatgg cgtgtggaca
      361 tgcggcagct atactggatc ggggctcgca ttatagataa caaggggagg gcgcttggcc
      421 cgacgctggg gggcgctggg cgcgttgtgc gtcgccatgg cggcggcggc gcctgccgca
      481 gcgcaggggg acgccgcgcc gcgcttcgag atccggcgct tcgtcatcga cggcgccacg
      541 ctggtgccgc aggcgcgcct gcgcgaggcg accgcgcctt tcacgggcgc ggggcgcgat
      601 ttcggcgacg tgcagcgcgc cctcgaggcg gtcgagcggc tgtacgcgga ggcgggctac
      661 agcgccgtgc aggtcctgct gcccgagcag gagctcgagc ggggcgaggt gcgcctgcgc
      721 gtcctcgagg cgcgcctggg ccgcgtgctg gtggagggca atcgccattt cgacgaggcc
      781 aacatccgcg caagcgtgcc cgcgctcgtg cccgggcagg cgcccaacat cctccaggtc
      841 ggccgcaacc tgcgcgtggc caatgagagt cccgccaagc aggcgacggt gctgctgcgc
      901 agcgggggcg aggaaaccac cgtcgacgcg gtcgtgcggg tcaccgatga gccgccgacc
      961 aaggtcagcg tgacgctcga caataccggg acgagcgaga cgggcaggtt gcgcttcggc
     1021 ctgggcttgc agcacgccaa cctcgccggc ggcgaccagg tcctcaccct gcagtacgtc
     1081 ggctcgccgc acgacgagga cgataccggc aacctcgcgc tcgagcccag cgagcgggtg
     1141 ctcatcgtcg gcgttggact gcgcatcccg ctgtacgcgc gcggcgacgc gctggacttc
     1201 accgccggct actcgaacgt cgactcgggc acggtcggtg gtctgtacaa cgtcagcggg
     1261 gcgggtggcc tgttcagcgc gcgctacacg cattacctgg accgggcggg cgactacgat
     1321 cagcgcgtcg cgctctcctg ggaccatcgc gcctacagtt tcaaggacgt gcgtgccatc
     1381 ggcagcaacg agcagctgca gcccgacatc accgtgcggc cgctgtcgct cgcctaccac
     1441 ggcctgctgc gccgcccgga cagcgaaacc gcggtgtcgg tcggcgtgtc gcgcaatctg
     1501 cccggcggca atgacggcag cccggaggac ttctgccagc tgccgcccaa tgccccgcat
     1561 ggggtctcgc gctcggacgg catgggcaac tgccccgacc cgtactacgt gctgtggcgc
     1621 tggtcgatca accacaacca ctcgctcgcc ggggactggc agatgcgctt cggcctgggc
     1681 ggccagtaca cccgcgacat gctcgtgtcg ggcgaaatgt tcgggctggg aggcgccgat
     1741 tccgtgcgcg gcttcgacga gcgggcgatc gccgacgaca tcggctaccg ggggacgctg
     1801 gagctgtact cgccggattt cggcgcctgg gccggctgga ccggcgcccg ggcgcgcgcg
     1861 ctcgtcttct acgactgggg tgcgctgcgc cgcaaccagc cggccccggg ggacgtggtg
     1921 gcgcagagca tcgccagcgt gggcgtggga ctgcgcatca cgcacggcac ccggctttcc
     1981 ctccgtgcgg actggggctg ggtggtggac gagggcggcg tgcccggcct cggcaccgac
     2041 ccgggaacct acaaggcgac cggggagggc cggctgcacg ccagcgtggc ttatctcttc
     2101 tagcagtgca ggttttggcc ggtttgctac catagccggc aacacacggc tcatggcaac
     2161 cacggtctcc ccggcagtcc tgcgcaccgt cccgctcttc gcgagctttt ccgaagagca
     2221 actgcgcgcg ctcgccacgc tcgtcacgcg gcgcagcgca tcgcgcggct ccatcatcgt
     2281 cgccgccggc gacccgaccg actccctgta catcatcctg tccggccgct tcaaggtgat
     2341 gatgagcgac gccgaaggca aggaagtgat tctcagcatc cttaccggcg gcgagttctt
     2401 cggcgagatg ggcctcatcg acgacgcgcc gcgctcggcg agcgtggtcg ccatcgagcc
     2461 ctgcgagctg ctggtgctca accggcgcga cttcaagaag tgcatgacgg agaacgccga
     2521 gatggccatg ggcgtcatgc gcggcctggt gcgccggttg cgcgaggccg accgcaagat
     2581 cggcagcctc gcgctgctgg atgtctacgg ccgggtcgcg cgcctgctgc tcgacatggc
     2641 cgagacggtc gacgggcaga agatggtcac caagcgcctg cccaagcagg acatcgccaa
     2701 gatgatcggc gcctcgcgcg agatggtcag ccgcgtcatg aaggacctgc agaccggcgg
     2761 ctatatcgag atgcgcggct cgagcatcct gctgcgcgat accatcgccc tgcccgacta
     2821 gcgggcggca atcccagcag aatcagcaat ttacgctccc cgagcgtgaa atagttcctc
     2881 gcggggaggc ctccgccctc cctagcatcg ctgcatcgtg aattccctcc gggtgctcct
     2941 tgcggccgcc agcctgctgg cgctgcccgc agtcgcgctg gcgggcgacg gcagcgtcgc
     3001 gcagctctcg ggtacgctga cggtcaagaa ggccgacggc tcggtgcgca tcctctcgcg
     3061 ccagtcgcag atcgagcagg gcgacacgct caccaccgag cgcgacagct acgcccagat
     3121 ccgcttcgcg gacggcggca gcctcaccct caagccgcgc accgccgtgc gcctggacca
     3181 gttccgcttc acgcaggaca agccgcgcga ggacgcgttc gccgtgcggc tgctgaaggg
     3241 cgggctgcgc gcggtcaccg gcggcatcgg caagcgcaat cccgagaatt acgaaatgac
     3301 caccgaaacc gccaccgtcg gcgtgcgcgg cacgaccttc tcggtggacg actgcgtgaa
     3361 cgcgcgcggc ggcgagtgcg cgcggctcgc gcccgcggtc tacgtgagcg tatccgacgg
     3421 cgaggtcgtg ctgcgcaacg gcgcgggcga gctcgccctg ggcgccggcc agttcggcca
     3481 gatcgtgcgc ggcgagcggc cgctgttcca gtcgaccgac ccggggctgc acttcgcgcc
     3541 gcccgcgacc ttcatccagt cgattctctc gggccggcag gtggccggca tcggcgacaa
     3601 gctggagtgc gccatcagcg cacggtagcg cctgcggtaa gattcgcgga cccgatggcc
     3661 cgcgatggca tctgcgccga aacccacagc agcgcccctt cccgctaagc tcgccagcct
     3721 gttgcgcgag gcccgctggc tcgtgctcgc agggcttgcc ggctacctgg cgctcatctt
     3781 cctgaccttc gatcgcggcg accccggctg gtcgcacagc gcgccggcgg ccaccctgca
     3841 taacgcgggc gggcgcgccg gcgcctggat cgcggatctg ctgctgtacc tgttcggcgt
     3901 ttcggcctac tggtgggtgg cgctgtgcct ggtcggcgtc gcctggggct accggcggct
     3961 cgagcgcagc gcgctcatcg accgccggcc gctcgccatc gccctgggcg gattcgcgct
     4021 gctgctgctg gccagcgcct cgctcgaggc gctgcgcctg cattcgctgg cggtagcgct
     4081 gccgctcgcg cccggcggga tgctcggcga cgccctgggc ggactcgccg cgcggctgct
     4141 cggcttcacc ggcgccacgc tgctgctgct gacgctggcg gcggtcggct tcagcctgtt
     4201 caccggcgtg tcctggctgg cggtcgccga ggcgacggga acgctcctgg agaacggcgc
     4261 ggcgctcgcc cggcgcgcct gggaaggccg cgcggaccgg cg
//
LOCUS       DASPGE010000182         3649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1571578, whole genome shotgun sequence.
ACCESSION   DASPGE010000182 DASPGE010000000
VERSION     DASPGE010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571578"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1495)
                     /locus_tag="VD791_05795"
     CDS             complement(155..1495)
                     /locus_tag="VD791_05795"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV07521.1"
                     /translation="MKPSLKRRLLVLALGATALVWLVTVLFTWLDARHEIHEVLDAHL
                     AQSASLLLVQLSHDIDEIDTEHAPLLHKDARKVAFQVWEEGTRLGLHSVNAPQRALGT
                     QQQGFSERTVEGSRWRVFTSWDASGEYLVHVGERMELREELATEMAMGLSLPLLAALP
                     LLGILLWIAVGRGLKPLAGLTGEVAQRDPDNLAPLDAARTPREVLPLVEQLNRLFGRI
                     AQSFERERRFTADAAHELRTPVAAIKAQAQVAQLASGAQARDHALERVVAGAERASRL
                     VEQLLTLARLDAIDRSALEACGLRTLAAQVVAEMAPAALARGVRLELADAPDVADVQV
                     RGIPALVEALLRNLVDNAVRHGAAGAVRVAVAREPGYAVVHVMDQGPGIPKHERDKVL
                     ERFYRLPDAGEGGSGLGLSIVQRIAEIHGATLALDSGEGGKGLQVEVRIPLAGP"
     gene            complement(1492..2157)
                     /locus_tag="VD791_05800"
     CDS             complement(1492..2157)
                     /locus_tag="VD791_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXV07522.1"
                     /translation="MRILVVEDDALLGDAIQAGLHELGNAVDWVRDGVAASHALETEP
                     YSAVVLDLGLPRLSGLQVLRKLRARGNPLPVLILTARDTVEDRIRGLDAGADDYLIKP
                     FDMGELAARLRALSRRAAGHPAPVLALAGVELDPAAHRVTYRGAPVELSVREFALLQE
                     LMLHAGRVLTRAQLEEKLYAWGEEIESNAVEVHIHRLRRKLYPELIQTVRGVGYMLPG
                     ERR"
     gene            complement(2220..2891)
                     /locus_tag="VD791_05805"
     CDS             complement(2220..2891)
                     /locus_tag="VD791_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HXV07523.1"
                     /translation="MSEPVLVWDVPTRVFHWLLALSFAGAFLTADTERLRDVHVLLGY
                     SMVGLIGFRIVWGLLGTRYARFRSFLFSPRELLQYVRSLLTRSPRHYLGHNPAGSLVI
                     FMLLALGIVTGASGLAVYNDVGGEWLEELHEGAAFAMLALVFVHIAGVLVASLLHREN
                     LVRAMITGRKRGEPGQGIRRGHAWVAALLLATVAAAWVWVPTQQPSAAAAAQERGHGD
                     REERG"
     gene            complement(3099..>3649)
                     /locus_tag="VD791_05810"
     CDS             complement(3099..>3649)
                     /locus_tag="VD791_05810"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV07524.1"
                     /translation="CPTMFVYEGYALAPTNRWQPTDAERRLGDPKILAMMRDLDVIPE
                     SQVPGWMRRARERNWQGWKPPHAAANLRRLWDEGITVVLGSDAGNIGTLHGPSVFREM
                     ERMVQAGLTPLETLRAATTNGAKATGKAGEAGVIAPGAHADLVLLDADPLQSIANLSR
                     IAWVIKGGRAYTVEELLAPVRQ"
BASE COUNT          574 a         1310 c         1246 g          519 t
ORIGIN      
        1 accgcgcagg tggtgggctc ggagaagatc gtggacggtc atccgggcaa gctgtccgcg
       61 gccgagatga aggcggtcaa gtcgcgcttc gacgcgctgc gcaaggccgc caacgcctcc
      121 aggaagccga aggcgagctg atgaggcggc ccggtcatgg gccggccagc ggaatccgca
      181 cttccacctg cagtcccttt ccgccctcgc cggaatccag ggcgagcgtc gcgccatgta
      241 tctccgcgat gcgctgcacg atcgaaagcc caaggccgct gccgccctcc cccgcgtcgg
      301 gcaggcgata gaagcgctcg agcaccttgt cgcgctcgtg cttggggatg ccggggccct
      361 ggtccatcac gtgcacgacc gcgtacccag gctcgcgcgc gacggcgacc cgcaccgcgc
      421 cggccgcacc gtggcgcacc gcattgtcaa cgaggttgcg cagcagcgcc tcgaccagtg
      481 ccgggatgcc gcgaacctgc acatcggcca cgtcgggcgc gtccgccagt tccaggcgca
      541 cgccgcgcgc gagcgccgcg ggcgccattt ctgccaccac ttgcgcggcc agcgtccgca
      601 gcccgcaagc ttcgagcgcg ctgcggtcga tggcgtccaa ccgcgccagc gtgagcagct
      661 gctcgaccag ccgcgacgcg cgttcggcgc ccgccacgac ccgctccagc gcgtgatcgc
      721 gcgcttgcgc gccgctcgcc agttgggcga cctgcgcctg cgccttgatc gcggccaccg
      781 gcgtgcgcag ctcgtgcgcc gcgtcggcgg tgaagcgccg ctcgcgctcg aacgactgcg
      841 cgatgcgccc gaagagacgg ttgagctgct cgaccagcgg cagcacttct cgaggtgtac
      901 gcgccgcgtc gagcggcgcg agattgtccg gatcgcgctg cgccacctcg ccggtcaggc
      961 cggccagggg cttcaggccg cgtcccacag cgatccacag caggatgccg agcagcggca
     1021 gcgccgccag caacggcagc gacaggccca tggccatctc ggtggccagc tcctcgcgca
     1081 gctccatgcg ctcgcccacg tgaaccagat actcgccgga ggcgtcccag ctcgtgaaca
     1141 cgcgccagcg gctgccctcc acggtgcgct cgctgaaccc ctgctgttgc gtgccgagcg
     1201 cccgttgcgg cgcgttgacc gagtgcaacc ccagccgcgt gccttcctcc cagacctgga
     1261 acgcgacctt gcgcgcgtcc ttgtgcagca gcggcgcatg ctcggtgtcg atctcgtcga
     1321 tgtcgtgcga cagctggacc agcagcagcg acgccgactg cgccagatgc gcgtcgagca
     1381 cttcgtggat ctcgtgccgg gcgtcgagcc aggtgaacag cacggtcacc aaccacacca
     1441 gcgcggtggc gccgagcgcc aggaccagca gccggcgctt gagcgagggc ttcaccgcct
     1501 ttcaccgggg agcatgtagc ccacgccgcg caccgtctgg atcagctcgg gatagagctt
     1561 gcggcgcagg cggtggatat gcacttccac ggcgttgctc tcgatctcct caccccaggc
     1621 gtagagcttc tcttcgagct gggcgcgggt gagcacgcgt ccggcatgga gcatcagctc
     1681 ctgcagcaaa gcgaactcgc gcaccgacag ctcgaccggc gcgccgcggt aggtgacgcg
     1741 atgcgcagcc gggtcgagct cgacgccggc caaggcgagg accggcgccg gatgcccagc
     1801 ggcgcggcgc gagagcgcac gcaggcgtgc ggccagctcg cccatgtcga acggcttgat
     1861 cagataatcg tccgcgcccg cgtccaggcc gcggatgcga tcctcgacgg tgtcgcgcgc
     1921 ggtaaggatc aacaccggca gggggttgcc ccgcgcgcgc agcttgcgca gcacctggag
     1981 gccggacagc cggggcagcc cgaggtcgag gaccacggcc gaatagggct ccgtctccag
     2041 cgcgtggctg gcggcgacgc cgtcgcgcac ccagtccacc gcgttgccca gctcgtgcag
     2101 gccggcctgg atggcgtcgc cgagcagcgc gtcgtcttcg actaccagta ttcgcatgag
     2161 cgggccacct gccctctaaa ggagcgaagg cggtcgtggg gccgggatag cgcggtagtt
     2221 tacccgcgct cttcccggtc gccgtgtccg cgctcctggg ccgcggcggc tgccgacggc
     2281 tgctgcgtcg gaacccacac ccaggcggcg gcgacggtcg cgagcagcag cgccgcaacc
     2341 cacgcatggc cgcgacggat accctgtccc ggttcgccgc gcttgcggcc ggtgatcatg
     2401 gcgcgcacca ggttctcccg atgcagcaag ctcgccacca gcacgccggc gatatgcacg
     2461 aacaccagcg cgagcatggc gaaggccgcg ccctcgtgca attcctccag ccactcgccg
     2521 ccgacgtcgt tgtagaccgc cagcccgctg gcgccggtga cgatgcccag cgcgagcagc
     2581 atgaagatca ccaggctgcc ggccggattg tgacccaggt agtggcgcgg cgagcgggtg
     2641 agcagggagc gcacgtactg cagcagctcg cgcggggaga acaggaacga gcggaagcgc
     2701 gcgtagcggg tgcccaacag cccccacacg atgcggaatc cgatcaagcc gaccatgctg
     2761 tagcccagca acacgtgcac gtcgcgcagg cgttccgtgt cggccgtcag gaaagcgccg
     2821 gcgaaagaca gcgccagcag ccagtggaaa actcgggtgg ggacgtccca caccaggacg
     2881 ggttcgctca tcgtcatctc ctcaggttgg cctcctgctg tgtcgatgga agcagcctag
     2941 agagccaacc ttaaggaatg cttatggcaa acctactacc aacgagcatt aggggagatg
     3001 gaggcgtgag gaagcgcctc tcccgttgcg ggccttgcgg gagaggcgcg ggtgagaggt
     3061 gtctacggag caggggtggg cacttcgcgc caccgttctt actgacgcac aggcgcgagc
     3121 agctcttcga cggtgtacgc ccgtccgccc ttgatcaccc aggcgatgcg gctgagattg
     3181 gcgatcgact gcagcggatc ggcatcgagc agcaccaggt cggcgtgcgc gccgggcgcg
     3241 atcacgccgg cttcgcccgc ctttcccgtc gctttcgcgc cgttggtcgt cgccgcgcgc
     3301 agcgtctcca gcggcgtcaa gccggcctgc accatgcgct ccatctcgcg gaacaccgac
     3361 ggcccgtgca gcgtgccgat gttgccggca tcgctgccca gcaccacggt aatcccctcg
     3421 tcccacagcc gccgcaggtt ggcggccgcg tgcggaggct tccatccctg ccagttgcgc
     3481 tcccgcgcgc ggcgcatcca gcctgggacc tggctctcgg gaatcacatc gagatcgcgc
     3541 atcatcgcca gtatcttcgg gtcgccgagc cgccgctccg cgtcggtcgg ctgccagcgg
     3601 ttggtggggg cgagcgcgta gccctcgtag acgaacatcg tcgggcagt
//
LOCUS       DASPGE010000183         6970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1220421, whole genome shotgun sequence.
ACCESSION   DASPGE010000183 DASPGE010000000
VERSION     DASPGE010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220421"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VD791_05815"
     CDS             <1..1071
                     /locus_tag="VD791_05815"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXV07525.1"
                     /translation="GAKTRISKYAVAIMTDNGLRGEYVTHWVGSSAALGQTLMLAPYL
                     IGRQAEEREQIYDDLKRQARQFDHMGHGPLDIALWDLHGKKLGVSVCEMLGGYRKRLP
                     AYASTYHGDRNGGLDSKEAFAAFALACYEIGYRAFKIHGWNDGNPREEAENLVHVRKA
                     VGDRMNLMIDPACELRTFADALHVGRACDDAGYFWYEDPFRDSGTSAFAHKKLREMIQ
                     TPILQTEHVRGVEPKADFLIQGGTDFLRSDPEYDMGITGAMKIAHLAEALGVDVEIHA
                     CGPAHRHCMAATRNSNYYEVALVGPDTPNAVPPVYRCGYSDQLDCIGKDGCVPVPTGP
                     GLGVDYDWEFIARNRVALHEFA"
     gene            complement(1112..1717)
                     /locus_tag="VD791_05820"
     CDS             complement(1112..1717)
                     /locus_tag="VD791_05820"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HXV07526.1"
                     /translation="MPSHRVRRAAALLALLACALTVFPPPGAAADADGQFGLAPLRGG
                     ELRWYYNHAGAPPLVSSARIVEAGEEAARAWAPCGVSITYLASTTAAPGVRDGMNVIG
                     WGRGLFNAVPFGDPGGMTLPWVEDGLAVEVDIVLVPQTVRTAVELRTVLMHEFGHAVG
                     LAHSPKPGSLMSASGSADDMDTHPRPSRAELQRCRNLHRRE"
     gene            complement(1726..2178)
                     /locus_tag="VD791_05825"
     CDS             complement(1726..2178)
                     /locus_tag="VD791_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HXV07527.1"
                     /translation="MSRLRLLMLLAVGACTLPAAAAVYKWTDAEGRIHYSDSPPPDRK
                     AAQVKIKINSISGPAVVSTLKGRPPAAKERVRIYTTTWCGYCKKAKAQLAARRVAFDE
                     IDVEASDSGRREFASLGGRGVPMILVGAQRMDGYDAGRLDAMLHSAGY"
     gene            complement(2245..3375)
                     /locus_tag="VD791_05830"
     CDS             complement(2245..3375)
                     /locus_tag="VD791_05830"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXV07528.1"
                     /translation="MSSAPLAAAGAPIPMLDLKAEYALFEPALTEALHGVLQSTQFVL
                     GPQMHALEREVAHYCGTQHGVAVANGTDAIHLALRALGIGPGHEVVAPSFTFIGTAEP
                     ISYTGATPVFVDIEPDTFNLDVAAVERAFTPRTRAVVAVHLFGQCADLDRLKSLCGKR
                     GVPLVEDCAQAIGADWGGRRCGSVGAAGCFSFYPSKNLGAYGDGGMVVTSDAKLAEEM
                     RVLRNHGSRERYHHHVVGYNCRLDEMQAAILRVKLAHLDRLSALRREKAQGYNRRLQG
                     SAVATPHEHGRGRHVYHQYTIKSARRDAIKAALDAAQIGSMIYYPIPLHRQEVYRASC
                     EGLRLPRSEEAAAQVLSLPMFPMLTDTQINRVCEVVARAAAA"
     gene            complement(3372..4346)
                     /locus_tag="VD791_05835"
     CDS             complement(3372..4346)
                     /locus_tag="VD791_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXV07529.1"
                     /translation="MLKPLRAAVIGAGYLGNFHAQKYAALDGATLEGVVDVDAQRAAA
                     VAHAVGARAFADYRELLGRIDVASIVVPTDLHYEVARTCLEAGVHILVEKPVTRTVQE
                     AAELAELAARRGRVFQVGHLERFNPAILAVRDQITRPLFIESDRLAVFKPRGTDVNVV
                     LDVMIHDIDLILSFARSEITAVRASGFPVLTSAVDIANARIEFADGCVADVSASRVSQ
                     QSLRKLRIFQPDQYLSIDCENFHVRTVRKEGSGIGPDGMPRIVSAEQRFEKADPLLAE
                     IGSFLEAVRTGQPPPVSGEDGRRALEVALEVCRQMNVTLSSVTQDKEL"
     gene            4581..5264
                     /locus_tag="VD791_05840"
     CDS             4581..5264
                     /locus_tag="VD791_05840"
                     /inference="COORDINATES: protein motif:HMM:NF042805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6781 family protein"
                     /protein_id="HXV07530.1"
                     /translation="MSEQDMSNEQIREAASASMASGENIRERVRALTLQALQSRRLDF
                     SAMREVMTSLTQGISLGAERRGQDVRQALSEAFAGMDQAMSKAAQASSLALKELAARG
                     RDFSDNELKQGLERMRQMEGDFLDSVRQVSRSTSGSVKSEWQDLIAHAQRAGTDTGRV
                     ISQTARDFSARMTATMSEGAVAGVEAARQFGERFAALTSGVLAGMSEALRPDKDKDED
                     KNKNKNKKA"
     gene            complement(5272..6630)
                     /locus_tag="VD791_05845"
     CDS             complement(5272..6630)
                     /locus_tag="VD791_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV07531.1"
                     /translation="MATAATSAGAGAPSAEMEILLRRVERERQARKQAEQLLEHKSRE
                     LYEANQRLHGQTARLELAVQERTRAMEEALSAMQEALRAKNEFLAVVSHEIRTPMNGV
                     LGTAQLLQMTEMTEEQCRYVEAIQSSGETLLALINDILDLSKLDSGKVYLDTRPLHLR
                     GLVKEVTELLRAQAQKKELYLDTEIAAEVPEWMIGDVHRIKQILTNLLGNAIKFTQHG
                     GVRVAMHMQQKGQVLVCQVHDTGIGIPPDRIEHLFEKFSQVDSSITRRQGGTGLGLAI
                     CKKLVEGMGGVIGVESKRHQGSTFTFRIPCTAAEPTLERRLLADVGLKTAVGLRVLLV
                     EDNAVNQMLALGMLQKLGCQADLAENGQEALQKVGAGYDLVLMDMQMPRMDGLTATRA
                     IRQMQLARQPYIVALTANAMESDRQLCLAAGMNDFLAKPFKAAELQEKLSDAATAAAA
                     TA"
     gene            complement(6738..>6970)
                     /locus_tag="VD791_05850"
     CDS             complement(6738..>6970)
                     /locus_tag="VD791_05850"
                     /inference="COORDINATES: protein motif:HMM:NF019320.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HXV07532.1"
                     /translation="REVRKLYADAELPRFAVLERKPNSMVLLYESPRHFADLAEGLIG
                     ACARHYGETLSIARENLASKAGSRVRFVLERR"
BASE COUNT         1095 a         2408 c         2381 g         1086 t
ORIGIN      
        1 ggcgccaaaa cgcgcatcag caaatacgcc gtggcgatca tgaccgacaa cggcctgcgc
       61 ggggagtacg tcacgcactg ggtcgggtcc tccgcggcgc tcggccagac cctgatgctc
      121 gcgccctatc tgatcgggcg ccaggcggag gagcgcgagc agatctacga cgatttgaag
      181 cgccaggccc ggcagttcga ccacatgggc cacggcccgc tcgacatcgc gctgtgggat
      241 ctgcacggca agaagctcgg cgtgtcggtg tgcgagatgc tgggcggtta ccgcaagcgt
      301 ttgccggcct acgccagcac ctaccacggc gaccgcaacg gcgggctgga cagcaaggag
      361 gcctttgctg cgttcgcttt ggcgtgctac gagatcggct atcgcgcctt caagatccac
      421 ggctggaacg acggcaaccc gcgcgaggag gcggagaacc tggtgcacgt gcgcaaggcg
      481 gtgggcgacc ggatgaacct gatgatcgat ccggcctgcg agctgcgcac cttcgccgac
      541 gcgctgcacg tgggccgcgc ctgcgacgat gccggttact tctggtacga ggatccgttc
      601 cgcgacagcg gcacctcggc gttcgcgcac aagaagctgc gcgagatgat ccagaccccg
      661 atcctgcaga ccgagcacgt gcgcggcgtg gagccgaagg ccgacttcct gatccagggc
      721 ggcaccgact tcctgcgctc cgatcccgag tacgacatgg gcatcaccgg cgcgatgaag
      781 atcgcgcatc tggccgaagc gctgggcgtg gacgtcgaga tccacgcctg cggtccggcg
      841 caccgccact gcatggccgc tactcgcaac agcaactatt acgaggtggc gctggtcggc
      901 ccggacaccc ccaacgccgt gccgccggtc taccggtgcg gctacagcga ccagctcgac
      961 tgcatcggca aggacggttg cgtgccggtg cccaccgggc cagggctggg cgtggactac
     1021 gactgggagt tcatcgccag aaatcgggtg gcgttgcacg agttcgcgtg aacccgcggg
     1081 gcgacggccg cgcatctcga tcggcggcgc gttactccct gcggtggagg ttcctgcagc
     1141 gctgcaactc ggcgcggctg gggcgcggat gcgtatccat gtcgtccgcc gaaccgctgg
     1201 cgctcatcag cgaccccggc ttcggcgaat gggcaaggcc cactgcatgt ccgaactcgt
     1261 gcatcaggac cgtgcgcagc tccaccgcgg ttcgaaccgt ctgcggaacc agaacgatgt
     1321 cgacttcgac cgcgagtcca tcctcgaccc agggcagcgt catgccgccg gggtctccga
     1381 agggcaccgc gttgaacaga ccgcgccccc acccgatcac gttcatgccg tcgcgcacgc
     1441 cgggtgccgc agtggtcgat gcgagatacg tgatgctgac gccgcaaggc gcccatgctc
     1501 gtgccgcctc ttcgccggcc tcgacgatgc gcgcgctgct caccagcggc ggagcgccgg
     1561 cgtgattgta gtaccagcgc agctcgccgc cgcgcagcgg tgcgagcccg aactggccgt
     1621 cggcatctgc cgctgcgccc ggcggcggga ataccgtgag cgcgcacgcg agcagtgcaa
     1681 gcagggcggc cgctcggcgg actcgatgcg acggcaacat cactctcaat acccggcgct
     1741 gtgcagcatc gcatccagcc ggccggcgtc gtagccgtcc atgcgctggg cgccgaccag
     1801 gatcatcggc acgccgcgcc cgccgagcga agcgaactcg cggcggccgc tgtccgaagc
     1861 ctccacatcg atctcgtcga aggcgacccg gcgtgccgcg agctgcgctt tcgccttctt
     1921 gcagtagccg caccaggtcg tggtgtagat gcgcacccgc tccttggccg cgggcgggcg
     1981 gcctttaagg gtcgacacca ccgccgggcc gctgatcgag ttgatcttga tcttgacctg
     2041 cgcggccttc cgatcgggcg gcgggctgtc cgagtagtgg atgcggccct ccgcatctgt
     2101 ccacttgtac accgccgcgg ccgcgggcag ggtacaggcg ccgaccgcca gcagcatcag
     2161 caaacgcaag cgcgacatgg gtgctccatg catgacctga aacccaggcg atgcaagtcg
     2221 caggcccgaa cctcgggaag tcgttcaggc cgccgctgcg cgcgccacca cctcgcacac
     2281 ccggttaatc tgcgtatcgg tcagcatcgg gaacatcggc agcgacagca cctgtgccgc
     2341 cgcctcttcc gaacggggca ggcgcaggcc ctcgcaggag gcgcgataca cctcctgccg
     2401 gtgcaacggg atcgggtagt agatcatcga gccgatctgc gccgcgtcga gcgccgcctt
     2461 gatcgcgtcg cgccgcgcgc tcttgatggt gtactggtgg tacacgtgcc gcccgcggcc
     2521 gtgctcgtgg ggggtggcca cggcgctgcc ctgcagccgc cggttgtagc cctgcgcctt
     2581 ttcccggcgc agcgcgctca ggcggtccag atgcgctagc ttgacgcgca ggatcgccgc
     2641 ctgcatctcg tcgagccggc agttgtagcc gacgacgtgg tgatggtagc gctcgcggct
     2701 gccgtgattg cgcagcactc gcatctcctc ggcgagcttc gcgtcggagg tgaccaccat
     2761 gccgccgtcg ccgtaggccc cgaggttctt gctcggatag aaggagaagc agccggccgc
     2821 accgacgctg ccgcagcgcc tgccgcccca gtcggcgccg atcgcctgcg cgcagtcttc
     2881 caccaggggc acgccgcgct tgccgcacag cgacttcaag cgatccagat cggcgcactg
     2941 gccgaacaga tgcacggcca ccactgcgcg ggtgcgcggg gtgaaggccc gctccaccgc
     3001 cgccacgtcc aggttgaagg tgtccggctc gatgtccacg aacaccggcg tggcgccggt
     3061 ataggagatc ggctcggcgg tgccgatgaa ggtgaaggac ggggcgacca cctcgtggcc
     3121 gggaccgatg ccgagcgcgc gcagcgcgag atgaatggcg tccgtcccgt tggcgaccgc
     3181 cacgccgtgc tgtgtgccgc aatagtgcgc cacctcgcgt tccagggcat gcatctgcgg
     3241 cccgaggacg aactgggtgg actgcagcac gccgtgcagc gcctcggtga gggccggctc
     3301 gaacagcgcg tactcggcct tcaggtcgag catcgggatc ggagcgcccg cggccgcgag
     3361 cggcgcgctg ctcacagctc tttgtcctgg gtcacggagc tgagcgtcac gttcatctgg
     3421 cggcacacct ccagggccac ctccagggcg cggcgcccgt cctcgcccga gaccggcggc
     3481 ggctggccgg tgcgcaccgc ctccaggaac gaaccgatct cggcgagcag cggatcggcc
     3541 ttctcgaacc tctgctcggc cgagacgatg cgcggcatgc cgtcgggccc gatgccggag
     3601 ccctccttgc gcacggtgcg cacgtggaaa ttctcgcagt cgatcgacag gtactgatcg
     3661 ggttggaaga tgcgcagctt gcgcagtgac tgctggctca cgcggctcgc cgagacgtcg
     3721 gccacgcagc cgtcggcgaa ctcgatgcgg gcgttggcga tgtccaccgc cgatgtgagc
     3781 accgggaagc cgctggcacg caccgccgtg atctcgctgc gcgcgaagct caggatcagg
     3841 tcgatatcgt ggatcatcac atccagcacc acgttcacgt cggttccgcg cggcttgaac
     3901 accgccagcc ggtcggattc gatgaacagc ggccgggtga tctggtcgcg cacggcgagg
     3961 atcgccgggt tgaaccgctc caggtgcccg acctggaaca cgcgcccgcg gcgagcggcc
     4021 agctccgcca gctcggcggc ctcctgcacg gtgcgcgtga ccggcttctc caccaggatg
     4081 tgcacgcccg cttcgaggca agtcctggcc acttcgtagt gcaggtcggt gggtacgacg
     4141 atcgaggcca cgtcgatgcg gccgagcagt tcgcgataat cggcgaacgc gcgtgccccg
     4201 acggcatggg cgaccgccgc ggcgcgctgc gcatcgacgt ccacgactcc ctccagggtg
     4261 gccccgtcga gggccgcgta cttctgcgcg tggaagttgc cgagatagcc ggcgccgatc
     4321 accgcagcgc gcaggggctt gagcatggga tgggcggggt cggaatcaat tgaggccggg
     4381 ccccagcggg cgccggcccg aaagcggtag gataaccgaa cgcggggcag tccagccggg
     4441 cacctggatt gtgtttgacg ccgggggctg tgtttggcag cgggggctcc gtctgcacac
     4501 cgggctccgg ttgacctgga tcagatgcct tgtcgcaggg acgcggcatg ctgcgacctg
     4561 tccccaacag gagatcgatc atgagcgagc aagatatgtc gaacgagcaa atccgcgaag
     4621 cggccagcgc ctcgatggcc tcgggcgaga acatccgcga gcgcgtgcgc gcgctcacgc
     4681 tgcaggcgct gcagtcgcgc cggttggact tctcggcgat gcgcgaggtc atgacctcgc
     4741 tgacgcaggg catcagcctc ggcgccgagc ggcgcgggca ggacgtgcgg caggcgctgt
     4801 ccgaggcctt cgccggcatg gaccaggcca tgagcaaagc cgcccaggct tccagcctgg
     4861 cgctgaagga gctggccgcg cgcggccgcg acttttcgga caacgagctc aagcaggggc
     4921 tggagcgcat gcgccagatg gagggcgatt tcctggacag cgtgcgccag gtgtcgcggt
     4981 cgaccagcgg cagcgtgaag agcgaatggc aggacctgat cgcgcatgcg cagcgcgccg
     5041 gcaccgatac cgggcgcgtg atctctcaga ccgcgcgcga tttctccgcg cgcatgaccg
     5101 ccaccatgag cgagggcgcg gtcgccggcg tggaagccgc gcgccagttc ggcgagcgct
     5161 tcgccgcgct caccagcggc gtgctggcgg gaatgtccga ggcgctgcgg ccggacaagg
     5221 acaaggacga ggacaagaac aagaacaaga acaagaaggc gtgactggct gtcacgcggt
     5281 cgcggcggca gcagtggccg cgtcgctcaa cttctcctgc agctcggcgg ccttgaacgg
     5341 cttggccagg aaatcgttca tgccggccgc caggcacagc tgccggtcgc tttccatcgc
     5401 attcgccgtc agcgccacga tgtagggctg gcgcgcgagc tgcatttgcc ggatggcgcg
     5461 ggtggcggtg agcccgtcca tccgcggcat ctgcatgtcc atcagcacca ggtcgtaccc
     5521 ggcgccgacc ttctgcagcg cttcctggcc gttctccgct aggtcggcct ggcagccgag
     5581 cttctgcagc atgcccaggg ccagcatctg gttcacggca ttgtcctcga ccagcagcac
     5641 gcgcaggccg accgccgtct tcaacccgac gtctgccagc aggcgccgct ccagcgtcgg
     5701 ctccgcggcc gtgcacggga tgcggaaggt gaaggtcgag ccctggtgcc gcttgctctc
     5761 cacgccgatc accccgccca tgccctcgac cagcttcttg cagatcgcca ggcccagccc
     5821 ggtgccgccc tggcggcgag tgatggagct gtccacctgg gagaactttt cgaacaggtg
     5881 ctcgatgcga tccggcggga tgccgatgcc ggtgtcgtgc acctggcata ccagcacctg
     5941 ccctttctgc tgcatgtgca tggccacgcg cacaccgccg tgttgggtga acttgatcgc
     6001 gttgcccaac aggttggtga ggatctgctt gatccgatgc acgtcgccta tcatccactc
     6061 cggcacctcg gcggcgatct cggtgtcgag gtacagctct ttcttctgcg cctgtgcgcg
     6121 cagcagctcg gtcacctcct tgaccagccc gcgcagatgc agcggacgcg tgtccagata
     6181 caccttgccg gagtcgagct tggacaggtc gaggatatcg ttgatcagcg cgagcagcgt
     6241 ctcgccgctg ctctggatcg cctccacgta gcggcactgc tcctcggtca tctcggtcat
     6301 ctgcagcagc tgggcggtgc cgagcacgcc gttcatgggc gtgcggatct cgtggctgac
     6361 caccgccagg aactcgttct tcgcgcgcag cgcctcctgc atcgcgctca acgcctcttc
     6421 catggcgcgc gtgcgctcct ggacggcgag ctccagccgc gcggtctgcc cgtgcaggcg
     6481 ctggttggcc tcgtacagct cacggctctt gtgctcgagc agctgctcgg cctgcttgcg
     6541 cgcctggcgc tcgcgctcca cgcgccgcag caggatctcc atctccgcgc tgggcgcgcc
     6601 ggcgccggcc gaggtggcgg ccgtggccaa tgccccggcg gataccccgg gagacactcc
     6661 agatgaaggt cccaacgacg ccccagacgg cccggacgac gccccagacg gcccggacga
     6721 cgctttggcg ttgccgctca tcgccgctcg aggacgaagc gcacccggct gcccgccttc
     6781 gaggcgagat tctcgcgcgc gatcgacaac gtctcgccgt aatgacgggc gcatgcgccg
     6841 atcaagccct cggcgaggtc ggcgaagtgg cgcggcgatt cgtacagcag caccatcgag
     6901 ttgggcttgc gctccagcac cgcgaaccgc ggcagctcgg cgtcggcgta tagcttgcgc
     6961 acctcgcggt
//
LOCUS       DASPGE010000184         4996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_166501, whole genome shotgun sequence.
ACCESSION   DASPGE010000184 DASPGE010000000
VERSION     DASPGE010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166501"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VD791_05855"
     CDS             complement(<1..1078)
                     /locus_tag="VD791_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07533.1"
                     /translation="MSGVAAFEPLSSFDFGEPWYRDRPVRWALALSLLLHAAALLWLP
                     GFRTPTPQDKPLTVELVQLPPEEPPPEEPVQRPKAASKPRPQAPVRPAPRAPARPELT
                     PPAPAPRPQTARPELQPRLQPPTPAQPQVAPAPTPTPLPPVARLEPLPERSPTVPEAR
                     PEPRPVIRPEPTPARIAPQAQPRQPPPTPQPRPEAAPQVRPKAAPTVAPRVEPRTAPA
                     PAPVEPRVEPRQEVIAVAPVPRPDSPPVQRVQPQPEIKPEVRADPRLEQAPQPRPDPR
                     PQAVSPPTPVTRPEVRPEPQRAAPQVQAPSQAPPEVAPAPRAEPQIAARAAPPPAAPV
                     PRPEMPVEARRDAAPAAVAPGAPAA"
     gene            1311..1382
                     /gene="pqqA"
                     /locus_tag="VD791_05860"
     CDS             1311..1382
                     /gene="pqqA"
                     /locus_tag="VD791_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HXV07534.1"
                     /translation="MWTKPEFTEMRFGFEVTMYIYNR"
     gene            1443..2357
                     /gene="pqqB"
                     /locus_tag="VD791_05865"
     CDS             1443..2357
                     /gene="pqqB"
                     /locus_tag="VD791_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HXV07535.1"
                     /translation="MRIRVLGAAAGGGFPQWNCNCRNCDGVRKGTIRAQPRTQSSICV
                     SPDGVDWVLFNASPDVLTQIKAFPALQPGRAIRDTGIRAIVLVDGQIDHTTGLLMLRE
                     GRPLEVWCTDMVHEDLTTGNPLFHILGHYAGVNRHRLPIEPGNEFAIDGIAHLRFTSV
                     ALKSKAPPYSPHRENPHQGDNVGLGITDTRTGRKLFYAPGLGEIEPRLEPLLAQADAV
                     MVDGTFWTDDEMIRLGISGKRARDIGHLPQSGAGGMIAVLRPLSARRKILIHINNTNP
                     ILDEDSPERAQLAQAGIEVAYDGMEIEL"
     gene            2367..3098
                     /gene="pqqC"
                     /locus_tag="VD791_05870"
     CDS             2367..3098
                     /gene="pqqC"
                     /locus_tag="VD791_05870"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HXV07536.1"
                     /translation="MGAPETLRQSSAQPWTRAEFEAQLRAKGGRYHIYHPFHIAMNGG
                     GCTREQVQGWVINRFYYQIAIPLKDAAIMSNCPDRDVRREWIQRIIDHDGQRGEEGGI
                     EAWLRLGEAVGLEREMLTSLKHVLPGVRFAVDAYINFARTRPWQEAVTSSLTELFAPE
                     IHQKRLDTWPEHYPWIDREGYYYFRKRLTEARRDVQFALNFTLDHYRTREQQERALDI
                     LQFKLDVLWSMLDSMQVNLGIGPKA"
     gene            3112..3405
                     /gene="pqqD"
                     /locus_tag="VD791_05875"
     CDS             3112..3405
                     /gene="pqqD"
                     /locus_tag="VD791_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HXV07537.1"
                     /translation="MDERVPLPPAAPIELHRQFRFQWEQAQNAYVLLYPEGMVRLAGS
                     AGEIMKRVDGKRSLEDIVKDLEAAFPGADLRADVVEFLEKAHGKGWIRTAQDR"
     gene            3368..4531
                     /gene="pqqE"
                     /locus_tag="VD791_05880"
     CDS             3368..4531
                     /gene="pqqE"
                     /locus_tag="VD791_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HXV07538.1"
                     /translation="MEKVGSAPRKIGNPLWLNAEITYKCPLHCVFCYNPTDYTQYGPE
                     LATEDWLRVFRQARELGAVQLGISGGEPLMRDDVEIMVAEASRLGYYVNLITSGVGLT
                     EKRIAAFKEGGLGQIQLSFQDSTRELNDFLSSTRTFELKSKVARLIKQYHYPMVLNVV
                     LHRLNIDHIQQILEMAEAMEAEHVELANTQYYGWAMVNREQLLPSREQLARAEEITNQ
                     FRARVGNKIKVYFVVPDYYANRPKKCMNGWGTTFINLTADGLALPCHEARMLPGLTFP
                     NVRDHDMRWIWYDSPGFNAYRGDGWMKEPCRSCPEKENDLGGCRCQAFMLTGDATNTD
                     PICDLSPRHDLVTEVVQRAQQPANPGKVQPIVFRDDRNSRRLSGKGEVVTPVA"
     gene            complement(4649..>4996)
                     /locus_tag="VD791_05885"
     CDS             complement(4649..>4996)
                     /locus_tag="VD791_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXV07539.1"
                     /translation="EAALDGKSAGDRCEVRLQPEDAFGDYQSGLVRTEPRRLFPPNLK
                     VGMQFEGSAEESGDTRIYTVTEIAEDKVVVDGNHPLAGQELVFSCVVTEVRTASAEEL
                     AHGHVHGAHGHHH"
BASE COUNT          842 a         1690 c         1719 g          745 t
ORIGIN      
        1 gtgccgccgg cgcgccaggc gcaacggcgg ccggtgcagc gtcgcggcgc gcttccaccg
       61 gcatctccgg acgcggcacg ggtgcggccg gcggcggcgc ggcgcgggcc gcgatctgag
      121 gttcggcgcg cggcgccgga gcaacctccg gcggcgcctg gctcggagcc tgtacctgcg
      181 gcgccgcacg ctgcggctcc gggcgcacct ccggccgggt caccggcgtc gggggactga
      241 ctgcctgcgg ccgcggatcg ggacgcggct gcggcgcttg ctccaggcgc ggatcggcac
      301 gcacctccgg cttgatctcg ggctgcggct gcacgcgctg aaccggggga ctgtcgggcc
      361 gcgggaccgg cgcgaccgcg atcacttcct gcctcggctc gacgcgtggt tcgaccggtg
      421 cgggcgccgg cgcggtgcgc ggctcgaccc gcggtgcgac ggtgggcgcg gccttcgggc
      481 gcacctgcgg cgccgcttcg ggacgcggtt gcggcgtcgg cggcggctgg cggggctgtg
      541 cctgcggtgc gatgcgtgcc ggcgtgggct ccggccggat gaccgggcgc ggctcgggac
      601 gcgcctcggg cacggtggga gagcgctcgg ggagcggctc gagccgcgcc acggggggca
      661 gtggcgtggg cgttggcgcc ggagcaacct gcggctgcgc cggcgtagga ggctgcagcc
      721 gcggctgcag ctcgggccgc gcggtctgcg ggcgcggtgc aggcgccggc ggcgtcagct
      781 ccggacgcgc gggcgcccgc ggcgccgggc gcacaggcgc ctgcggcctg ggcttggacg
      841 cagcctttgg ccgctgaacc ggctcctccg gaggcggttc ctccggggga agctgcacca
      901 gctccaccgt gagcggcttg tcctgcgggg tgggggtgcg gaaacccggc agccacagca
      961 gcgctgcggc gtgcagcagc agcgacaggg cgagcgccca cctcaccggc cgatcgcgat
     1021 accagggctc gccgaaatcg aacgagctca gcggctcaaa cgcggcgacg ccggacatcc
     1081 tgcaagcgcg aaagctgcgc gcggcgtgac agatgtgagc agattctcat gattatcatg
     1141 agctggaaat gctgattttt gtatgggagc cgggacttgg ctcaccgaca atgacatccg
     1201 gacgcggagc ccgtcgtccg ctcatcaggc ggccggcaca actccgggtt cccaaagctg
     1261 ggcgagccca gcgggatcga gaatgataac ccctaacact ggagaagagt atgtggacca
     1321 agcctgaatt caccgaaatg cgcttcggct tcgaagtgac gatgtacatc tacaaccgct
     1381 aactcagcag cggcagacaa aagcccccgc tgcggggctt ttgtcttttg gagactgggc
     1441 gaatgcgcat ccgcgtgctg ggcgccgctg ccggcggcgg cttcccgcaa tggaactgca
     1501 actgccgcaa ctgcgacggt gtgcgcaagg gcacgatccg tgcccagccg cgcacccagt
     1561 cctcgatctg cgtgtcgcct gacggtgtcg actgggtgct gttcaacgcg tcgccggacg
     1621 tgctgacgca gatcaaggcc ttcccggccc tgcagccggg ccgggcgatc cgcgacaccg
     1681 gcatccgcgc catcgtgctg gtggacgggc agatcgacca caccaccggc ctgctgatgc
     1741 tgcgcgaggg ccgtccgctc gaggtctggt gcaccgacat ggtccacgaa gacctcacca
     1801 ccggcaaccc gctgttccac atcctcgggc actatgccgg cgtgaaccgc caccggctgc
     1861 cgatcgagcc gggcaacgag ttcgccatcg acggcatcgc gcacctgcgc ttcaccagcg
     1921 tagcgctcaa gagcaaggcc ccgccctact ccccgcaccg cgagaacccg caccagggcg
     1981 ataacgtcgg cctgggcatc accgacaccc gcaccgggcg caagctgttc tatgcgcccg
     2041 ggctggggga gatcgagccc cgtctcgagc cgctgctggc gcaggccgac gcggtgatgg
     2101 tggacggcac cttctggacc gacgacgaga tgatccgtct ggggatctcc ggcaagcgcg
     2161 cgcgcgacat cgggcatctg ccgcagtccg gcgcgggcgg catgatcgcg gtgctgcgcc
     2221 cgctgtcggc gcggagaaag atcctgatcc acatcaacaa caccaatccc atcctggacg
     2281 aagattcgcc cgagcgcgca cagctcgcgc aagcgggcat cgaggtcgcg tacgatggaa
     2341 tggagatcga gctgtaggag acgcatatgg gcgcaccgga aacccttcgc cagtcgagcg
     2401 cgcaaccctg gacgcgcgcg gagttcgaag cgcagctgcg cgcaaagggc ggccgctacc
     2461 acatctatca tccgttccac atcgccatga acggcggcgg ctgcacgcgc gaacaggtgc
     2521 agggctgggt gatcaaccgc ttctactacc agatcgccat ccccctgaag gacgcggcga
     2581 tcatgtccaa ctgcccggat cgcgatgtgc gccgcgagtg gatccagcgc atcatcgatc
     2641 atgacgggca gcgcggcgag gaaggcggca tcgaggcgtg gctgcgcctg ggcgaagcag
     2701 tgggactcga gcgcgagatg ctgacttcgc tcaagcacgt gctgccgggc gtgcgcttcg
     2761 cggtggacgc ctacatcaac ttcgcgcgca cacggccgtg gcaggaggcg gtcacctcct
     2821 cgctcaccga gctgttcgcc ccggagatcc accagaagcg cctggacacc tggcccgagc
     2881 actatccgtg gatcgaccgc gagggctact actacttccg caagcgcctg accgaggcac
     2941 ggcgcgacgt gcagttcgcg cttaacttca cgctcgacca ctaccgcacg cgcgagcagc
     3001 aggagcgcgc cctcgacatc ctgcagttca agctggacgt gctgtggtcg atgctggatt
     3061 cgatgcaggt gaacctcggc atcgggccga aggcctgatc cgccccggaa catggacgag
     3121 cgcgttccac tgccgccggc ggcgcccatc gaactgcatc gccagttccg gttccaatgg
     3181 gaacaggcgc agaatgccta tgtgctgctg taccccgagg gcatggtccg gctggcgggc
     3241 agcgccggcg agatcatgaa gcgggtggac ggcaagcgca gcctggagga catcgtcaag
     3301 gatctcgagg cggcgtttcc gggagcggac ctgcgcgccg atgtcgtcga atttctggag
     3361 aaggctcatg gaaaaggttg gatccgcacc gcgcaagatc ggtaacccgc tgtggctcaa
     3421 cgcggaaatc acctacaagt gcccgctgca ctgcgtgttc tgctacaacc ccaccgacta
     3481 tacccagtac gggcccgagc tcgccacgga agactggctg cgcgtgttcc ggcaggcgcg
     3541 cgagctgggg gcggtgcagc tgggcatctc gggcggcgag ccgctgatgc gcgacgacgt
     3601 cgagatcatg gtagcggagg cgagccggct cggctattac gtcaacctga tcacctcggg
     3661 cgttgggctc accgagaaac gcatcgccgc cttcaaggaa gggggcctcg ggcagatcca
     3721 gctctcgttc caggactcca cccgcgagtt gaacgacttt ctgtccagca cccggacctt
     3781 cgagctcaaa tccaaggtcg ccaggctgat caagcagtac cactacccga tggtgctcaa
     3841 cgtggtgctg caccggctca acatcgacca catccagcag atcctggaga tggccgaagc
     3901 gatggaggcc gagcacgtcg agctcgccaa cacccagtac tacggctggg cgatggtgaa
     3961 ccgcgagcag ctgctgccga gccgcgagca gctggcgcgc gccgaggaga tcaccaacca
     4021 gttccgcgcg cgcgtgggca acaagatcaa ggtgtacttc gtggtgccgg actactacgc
     4081 caaccggccg aagaaatgca tgaacggctg gggcaccact ttcatcaacc tgaccgccga
     4141 cgggctggcc ctgccctgcc acgaggcgcg catgctgccg gggctgacct tccccaacgt
     4201 gcgcgaccat gacatgcgct ggatctggta cgactcgccc gggttcaacg cctaccgcgg
     4261 cgacggctgg atgaaggagc cgtgccggtc ctgcccggag aaggagaacg acctcggcgg
     4321 ctgccgctgt caggccttca tgttgaccgg ggacgcgacc aacaccgatc ccatctgcga
     4381 cctgtcgccg cgccatgatc tggtgaccga ggtggtgcag cgggcgcagc aacccgctaa
     4441 cccgggcaag gtgcagccca tcgtgttccg cgacgaccgc aactcgcgca ggctgagcgg
     4501 aaagggcgag gtagtcactc ccgtggcgtg acgccgcggc agcgtactgc tctctaaccg
     4561 cagaggcgca aggggccgca gaggaatgct cttgcggttt tcctctgcga aactgcgcct
     4621 ttacgcctct gcggccaagc ggtaatactc aatgatggtg cccatgcgca ccatgcacgt
     4681 ggccgtgcgc gagctcctcc gcgctcgcgg tgcgcacctc ggtgaccacg cacgagaaca
     4741 ccagctcttg accggccagg ggatggttgc cgtccaccac caccttgtcc tcggcgatct
     4801 cggtcaccgt atagatgcgc gtatcgcccg actcctcggc gctgccttcg aactgcatgc
     4861 ccaccttgag attgggcgga aacaggcgcc gcggctcggt gcgcaccagg ccggattggt
     4921 aatcgccgaa ggcgtcctcg ggttgcaagc gcacctcgca gcggtcgccg gcgctcttgc
     4981 cgtcgagcgc cgcttc
//
LOCUS       DASPGE010000185         5262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_167170, whole genome shotgun sequence.
ACCESSION   DASPGE010000185 DASPGE010000000
VERSION     DASPGE010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167170"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VD791_05890"
     CDS             complement(<1..816)
                     /locus_tag="VD791_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07540.1"
                     /translation="MTNVAGGARWWVAGDLNGFFGLFTNVLLNVIVLTTLCLYVVQLP
                     PDTVYGRVLPALGIALPLGNLFYAYLAWQLAKKEGRSDVTAMPYGPSVPHMFIVVLVI
                     MLPTYLATNDPMLAYQAGLAWCFIIGVIVLIGAFVGPWIRKVTPRAAMLGTLAGISIA
                     FISMRPAFQMWEVPWIGLACFAIVLIAWTANKRLPGGIPGGLAAVILGAAIGWIAAAF
                     GWSDYMKGEQVTKSFESFGLHLPFFSSHVFSGLAIIGPLLATAIPLGIYNFTEA"
     gene            complement(869..1822)
                     /gene="arcC"
                     /locus_tag="VD791_05895"
     CDS             complement(869..1822)
                     /gene="arcC"
                     /locus_tag="VD791_05895"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HXV07541.1"
                     /translation="MSKGTRIAVVAVGGNALILGPGKESIRDQAAAAEITCRYVADIV
                     EQGWTVVLTHGNGPQVGFILRRSGIAKGQVPEVPVDYADADTQGAIGYMFQRGLYNEL
                     QRRGVSRNVVALVTQVRVAPDDPAFQRPTKPIGAHMDEDTAKHLAQQLGWTVAEDAGR
                     GWRRVIASPEPKEIVELDAIRQLVTQGFVLIACGGGGIPVMRDERGDLRGLEAVIDKD
                     LASSLLAQELGAQLFVVSTSVERVAIHFNTPQQQWLDDISLADLQRYAAEGHFPAGSM
                     GPKIEAIRRYLTAAPQGRAIITDPPNLGRALAGATGTRVHR"
     gene            complement(1819..3228)
                     /locus_tag="VD791_05900"
     CDS             complement(1819..3228)
                     /locus_tag="VD791_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HXV07542.1"
                     /translation="MSLWFKDKPSIVNVGLASFADSARECGARVTQIEWQPPAEGDRE
                     AGMALARLVGAARVEQANEQAYAAYLNAQPALIGVGVAHEAVPGLQGRCIAHAGPPIA
                     WPEMCGPMQGAILGACLLEGWARDLSEAERLARSGGIAFSPCHHHDAVGPMAGVISPS
                     MPVWIVENAVSGARCFSNMNEGLGKVLRFGANGPEVIERLRWMAETLGPALAAALQRA
                     GPIELKPLMAQALHMGDEVHNRNAAASGLLLKRLLPALVESGLPGDTVAKAVRFAADN
                     DHTFLNLSMAACKSMLDAAHGVVGSSMVTVMARNGVRFGVRLSGTGESWFEADAPQVD
                     GLYFSGYGPGDAAGDLGDSAITETAGLGGFAMAAAPAIVQFVGGTPADAVATTQSMRH
                     ITLGRNTAFTLPALSFTGTPAGIDARKVLDTGILPVINTGIAHKQAGIGQIGAGVTAA
                     PLACFIQAVRALDAQMVRS"
     gene            complement(3225..4760)
                     /gene="fdrA"
                     /locus_tag="VD791_05905"
     CDS             complement(3225..4760)
                     /gene="fdrA"
                     /locus_tag="VD791_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HXV07543.1"
                     /translation="MAKFHVLRKTYRDSVTLMQLASKLARLPGISSASASMATPANLD
                     LLREAGTDVSGVQAGPNDLLIMLAGEPAAVDAALAEAQKLLSDRGEREEQDERGSGQR
                     RAPRSIAMALAEAPAGLALISVPGEYAAAEALKAVNLGLNVMLFSDNVTLDDEVMLKR
                     QADARGLIVMGPDCGTAIVGGVPLAFANKVRAGPIGLVGASGTGLQQVTVLIDRMGSG
                     ITQAIGTGGRDLKQEVGGLSMLAGVRALAADPDTRVIVLISKPPAPEIAQRILGEARG
                     AGKPVVVNFLGAQPAEASGGNLHSAYTLEDAARAAVALAEGRAPGQAPQPGAAGTAPK
                     LKPEQRYVRGLYSGGTFCYEATLLLLEAQIGEVHSNTHVGRARDLADPWRSVGHTLVD
                     LGDDVFTRGRPHPMIDHRLRNERIVQEAADPSVAVILLDVVLGYGSHPDPAAEMRAAL
                     AQARDVARKAGREIAFVGQVCGTEADPQNLDRQSAALAGMGMLLANSNAQAVHLAARI
                     VQR"
     gene            complement(4753..>5262)
                     /locus_tag="VD791_05910"
     CDS             complement(4753..>5262)
                     /locus_tag="VD791_05910"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HXV07544.1"
                     /translation="RPSPARGEGRVLRVRLIALGEAHRDGSAQAIAHAVHGLVGLGPG
                     LTPAGDDALIGWLAGTALLGPDRRSELLCQAVRARLARTTDVSRAHLEDALAGAFSEP
                     LAQFANALVRGPGEAQRALADLAAVGATSGLDAAAGLLAAVETAAAPRPYDAEPCPPR
                     PYQPEPCDG"
BASE COUNT          747 a         1925 c         1821 g          769 t
ORIGIN      
        1 cgcctcggtg aagttgtaga tgcccaatgg aatggcggtg gcgagcagcg gaccgatgat
       61 cgccaggccg ctgaagacgt gcgacgagaa gaacggcagg tgcaagccga agctctcgaa
      121 cgacttggtc acctgctcgc ccttcatgta gtcgctccag ccgaacgccg cggcgatcca
      181 gccgatcgcg gcgcccagga tcaccgccgc caggcccccg ggaatgccgc ccggcaggcg
      241 cttgttggcg gtccaggcga tcagcacgat ggcgaagcag gccagcccga tccacggcac
      301 ttcccacatc tgaaaggcgg gacgcatcga gatgaacgcg atcgagatcc cggccagcgt
      361 ccccagcatc gccgcgcgcg gggtgacctt gcggatccac ggtccgacga acgccccgat
      421 cagcacgatc acgccgatga tgaagcacca cgccaggccg gcctggtagg cgagcatcgg
      481 gtcgttggtg gccaggtagg tgggcagcat gatcaccagc actacgatga acatgtgcgg
      541 cacgctcggc ccgtagggca tcgccgtcac gtccgagcgg ccctccttct tggcgagctg
      601 ccaggccaga taggcgtaga acagattgcc cagcggcagc gcgatcccca gcgccggcag
      661 cacccgcccg tacacggtgt cgggcggaag ctgcaccacg tacaggcaca gcgtggtcag
      721 gacgatcacg ttgagcagca cgttggtgaa caacccgaag aacccgttca agtcgcccgc
      781 cacccaccag cgggcacccc ctgcgacgtt cgtcatggtc ctctccctcc tgttgtggtt
      841 gtggtgaact gcccagcccg gatatttctc agcggtgaac gcgtgtgccg gtcgctcctg
      901 ccagggcccg ccccaggttg ggcgggtcgg tgatgatcgc gcgcccctgc ggggcggcgg
      961 tgagatagcg gcggatggct tcgatcttgg gccccatgct gcccgcgggg aagtgacctt
     1021 cggcggcgta tcgctgcagg tcggcgagtg agatgtcgtc gagccattgc tgctgcggcg
     1081 tgttgaagtg gatcgccacg cgctccaccg acgtggacac cacgaacaac tgcgcgccca
     1141 gctcctgcgc gagcaggctg gaggcgaggt ccttgtcgat cactgcttcc aggccgcgca
     1201 ggtcgccgcg ctcgtcgcgc atcaccggga tgccgccgcc gccgcaggcg atcagcacga
     1261 agccctgcgt caccagctgg cggatggcgt ccagctcgac gatctccttc ggctcgggcg
     1321 aggcgatcac ccgccgccag ccgcggccgg cgtcctcggc caccgtccag cccagttgct
     1381 gcgcaagatg cttggcggtg tcctcgtcca tgtgcgcgcc gatcggcttg gtcgggcgct
     1441 ggaacgcggg gtcgtcgggc gccacgcgca cctgcgtgac cagcgccacc acgttgcgcg
     1501 agacgccgcg gcgctgcaac tcgttgtaca ggccgcgctg gaacatgtag ccgatcgcac
     1561 cctgggtgtc ggcgtcggcg taatccaccg gcacctccgg cacctggcct ttggcgatgc
     1621 ccgagcggcg caggatgaag ccgacctgcg gcccgttgcc gtgcgtgagc accacggtcc
     1681 agccctgttc gacgatgtcc gcgacgtaac ggcaggtgat ttccgcggcc gccgcctgat
     1741 cgcggatcga ctccttgccc gggccgagga tcagcgcgtt gccgccgacc gccaccaccg
     1801 cgatgcgcgt gcccttgctc atgagcgcac catctgcgcg tcgagcgcgc gcaccgcctg
     1861 gatgaagcag gcgagcggcg ccgcggtgac gcccgcgccg atctggccga tgccggcctg
     1921 cttgtgcgcg atgccggtgt tgatcaccgg caggatgccg gtgtcgagca ccttgcgcgc
     1981 gtcgatgccg gccggcgtgc cggtgaaact cagcgccggc agcgtgaacg ccgtgttgcg
     2041 gccgagcgtg atgtgccgca tcgactgcgt ggtggcgacc gcgtcggccg gcgtgccgcc
     2101 gacgaactgc acgatcgccg gggcggccgc catggcgaag ccgcccaggc ccgcggtctc
     2161 cgtgatcgcc gaatcgccca ggtcgccggc cgcgtcgccc gggccgtaac cgctgaagta
     2221 caggccgtcc acctgcggcg cgtcggcctc gaaccagctc tctccggtgc cggacaggcg
     2281 aacgccgaag cgcacgccgt tgcgcgccat gaccgtcacc atgctgctgc ccaccacgcc
     2341 atgcgctgca tccagcatgc tcttgcacgc ggccatcgac aggttgagaa aggtgtggtc
     2401 gttgtcggcc gcgaagcgca ccgccttggc gacggtgtcg ccgggcagcc cggactccac
     2461 cagcgcgggc agcagccgct tgagcagcag cccgctggcc gcggcgttgc ggttgtgcac
     2521 ctcgtcgccc atgtgcagcg cctgcgccat cagcggcttc agctcgatcg gcccggcccg
     2581 ttgcagcgcg gcggccagcg ccggcccgag cgtttccgcc atccagcgca ggcgctcgat
     2641 cacctccgga ccgttggcgc cgaagcgcag caccttgccc agtccctcgt tcatgttcga
     2701 gaagcagcgc gcgccgctca ccgcgttctc cacgatccac accggcatcg acgggctgat
     2761 gacgccggcc atcggaccga cggcgtcatg atgatggcag ggcgagaagg cgatgccgcc
     2821 gctgcgcgcg agccgctcgg cctcggacag gtcgcgcgcc cagccctcca gcaggcaggc
     2881 gccgaggatc gcaccctgca tcggcccgca catctccggc caggcgatgg gcggtcccgc
     2941 gtgcgcgatg caacggccct gcagaccggg caccgcctcg tgcgcgacgc ccacgccgat
     3001 cagcgccggc tgcgcgttga gataggcggc gtaggcctgc tcgttcgcct gctcgacccg
     3061 tgccgcgccc accagccgcg ccagcgccat gccggcttcg cgatcgccct ccgcgggcgg
     3121 ctgccattcg atctgcgtga cccgcgcgcc gcactcgcgc gccgagtccg cgaacgacgc
     3181 cagtccgacg ttcacgatgc tgggcttgtc tttgaaccag aggctcatcg ctgcacgatc
     3241 cgcgccgcca ggtgcacggc ctgcgcgttg ctgttggcga gcagcatccc catgccggcc
     3301 agcgcggcgg actggcggtc caggttctgc ggatcggcct cggtgccgca cacctggccg
     3361 acgaaggcga tctcgcggcc ggccttgcgc gcgacatcgc gcgcttgcgc gagcgcggca
     3421 cgcatctcgg cggccggatc gggatggctg ccgtagccca gcaccacgtc gagcaggatg
     3481 acggcgacgc tcggatcggc cgcctcctgc acgatgcgct cgttgcgcag gcgatggtcg
     3541 atcatcgggt gcgggcggcc gcgcgtgaac acgtcgtcgc ccaggtccac cagcgtgtgc
     3601 ccgacgctcc gccagggatc ggcgagatcg cgcgcgcggc ccacgtgcgt gttcgaatgc
     3661 acctcgccga tctgcgcctc gagcagcagc agcgtggcct cgtagcagaa ggtgccgccg
     3721 ctgtagaggc cgcgcacgta gcgctgctcc ggtttcagtt tcggcgccgt tccggcagcg
     3781 cccggctgcg gcgcctgccc gggcgcacgc ccctcggcca acgcgacggc ggcgcgggcc
     3841 gcgtcctcca aagtgtacgc actgtgcaga ttgccgccgc tcgcctccgc gggctgcgcg
     3901 ccgaggaagt tgaccaccac cggcttgccg gcgccgcgcg cctcgcccag gatgcgctgc
     3961 gcgatctccg gggcgggcgg cttggagatc agcacgatca cgcgcgtgtc gggatcggcg
     4021 gcgagcgcgc gcacgccggc gagcatcgac aggccgccca cctcctgctt gagatcgcgc
     4081 ccgccggtgc cgatcgcctg cgtgatgccg ctgcccatgc gatcgatgag cacggtcacc
     4141 tgctgcaggc ccgtgccgga ggcgcccacc agaccgatgg ggcccgcgcg caccttgttg
     4201 gcgaacgcga gcggcacgcc gccgacgatg gccgtgccgc aatcggggcc catcacgatc
     4261 aggccgcgcg catcggcctg gcgcttgagc atcacctcgt cgtcgagcgt cacgttgtcg
     4321 gagaacagca tgacgttcag ccccaggttc accgccttga gcgcctcggc ggcggcatac
     4381 tcgcccggca ccgagatcag cgccagcccg gcgggcgcct cggccagcgc catggcgatg
     4441 ctgcgcgggg cacgccgctg gccgctgcca cgctcgtcct gctcttcgcg ctcgccccgg
     4501 tcggacagca gtttttgcgc ttcggcgagc gccgcgtcga ccgcagccgg ctcgcccgcc
     4561 agcatgatca gcagatcgtt ggggccggcc tgcacaccgc tcacgtcggt gcccgcctcg
     4621 cgcagcagat ccaggttggc cggggtggcc atgctcgccg aggcgctgct gatgccggga
     4681 aggcgcgcca gcttcgaggc gagctgcatc agcgtcaccg agtcgcggta ggtcttgcgc
     4741 aggacgtgga acttagccat cgcacggctc tggttggtac ggccttgggg ggcacggctc
     4801 cgcgtcgtac ggtcttgggg cggccgcggt ttccacggct gcgagcagcc ccgcagcggc
     4861 gtccagcccc gaggtcgcgc ccacggcagc gagatccgcc agcgcgcgct gcgcttcgcc
     4921 ggggccgcgc accagcgcgt ttgcgaactg cgccagcggt tccgagaacg cgccggccag
     4981 tgcgtcttcg agatgcgcac ggctcacgtc cgtggtgcgc gcgaggcggg cgcgcaccgc
     5041 ctggcacagc aactcgctgc gtcggtcggg gcccagcagc gcggtgccgg cgagccaacc
     5101 gatcagagcg tcgtctcccg ccggtgtgag acctggcccc aggccgacga gaccgtgcac
     5161 cgcgtgcgcg atcgcttgcg cgcttccgtc gcgatgcgct tcgccgagcg cgatgagccg
     5221 cacgcgcaat acgcgcccct ctccccttgc gggagagggg cg
//
LOCUS       DASPGE010000186         6853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_518878, whole genome shotgun sequence.
ACCESSION   DASPGE010000186 DASPGE010000000
VERSION     DASPGE010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518878"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VD791_05915"
     CDS             complement(<1..481)
                     /locus_tag="VD791_05915"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001204.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HXV07545.1"
                     /translation="MATYAIGDIQGCFEPLQRLLDEVGFDAGSDRLWLVGDLVNRGPD
                     SLATLRFVRSLGEAAVVVLGNHDLHLLAVAEGYEKLKREDSLQEVLDAPDRDTLLDWL
                     RHCPLMHFEYGWAMVHAGLLPGWSVSRALELAAEVEAALRAATYRSFLARMYGNQPQR
                     "
     gene            complement(553..1119)
                     /locus_tag="VD791_05920"
     CDS             complement(553..1119)
                     /locus_tag="VD791_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV07546.1"
                     /translation="MSEIHIQEHASPIKNWKQVAVLAALAFVVPVLLIVSLVQIVTGG
                     MRVDAGAPDMSEEAIAARIKPVGELNLGPGGAAEAPATAAPAAPLPAPAAAVQPAAAA
                     TAPAAAVRSGEEVHQQACAMCHVPGLAGAPRTGDKTAWQARIAQGKATLYEHAIKGFR
                     TMPAKGGNLSLSDAEVRAAVDYLAAQAR"
     gene            1246..1321
                     /locus_tag="VD791_05925"
     tRNA            1246..1321
                     /locus_tag="VD791_05925"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1279..1281,aa:Arg,seq:ccg)
     gene            1371..3368
                     /locus_tag="VD791_05930"
     CDS             1371..3368
                     /locus_tag="VD791_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07547.1"
                     /translation="MKPLQVSLFVLSAWSVAGAAFAWVYPEHREIALLAVRDLDASRR
                     AVFDGLWREARITQEKRLCEQGADAAQGLAPSCIDWAALSAIAGDHSCSSQQMTATVL
                     ESDWILVVADVAAQLKLDLSKIDVLPPVSQAPGSKDLVTDLRRRVQGEAARAARINAL
                     RTSDNRLQHADPKLATRAASNNAHFLLPRPSTGVSGKEYAQLVLKSGSEVSALGVYTW
                     FHLSALQKATRLASERERLAPEERQALARAMLFDEAFALHFLEDVFAAGHVAGTWGDA
                     SQRKGTHDFYNEAGLEVFLWKGDGASMVLMGDAHMRPEDAERASTVVRASLEQILDAA
                     AGQARDGSLPYTSDVPGQPDAFDVCKNNTIAQRPEAAQVPEGYWDAYAVGLAEVLRPT
                     PVPGLGPGLGAMPRFRSEVGPFVGLAAMLDGRWLDDAFTASDGSGLIGGVELSARVGL
                     GLEGVMGDAGDGLVFLALGLRGDASSTNSVDDSAAAKAGGNLTAAIPSRTGIATRLRM
                     PFYLIPGDLILLSPLLLVAPEKYAGIAVTAANGGLIPWQSGMATRIGRFQFVLGRELG
                     VTFYGYIGEDRVIAPGATPGEPTRLVDYKSTLFDLPILEYRTYRSFASEQSSTLLFQL
                     FASADVPHSAQTVSPVGAPTPDLKTVWSVGLRLLFDWRYYP"
     gene            3426..3842
                     /locus_tag="VD791_05935"
     CDS             3426..3842
                     /locus_tag="VD791_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HXV07548.1"
                     /translation="MQDLVTLPTLFLSSFLAATLLPGGSEVVLLAVIAREPALLWPAL
                     GWATLGNTLGGFSSYLLGRLLPQREKPSAAAARKARDWLQRFGAPALLLSWAPVVGDA
                     LCVAAGWLRINALAVSLFLALGKFLRYWAVSQAALP"
     gene            3919..>6853
                     /locus_tag="VD791_05940"
     CDS             3919..>6853
                     /locus_tag="VD791_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HXV07549.1"
                     /translation="MTGRLREIPYNYTSFSDREIVIRILGARAWALLDELRSQRRTGR
                     SARMLYEVLGDIWAVDRNPYLQDDLLANRHRLDALIQAMRHRLREIEARRLNPDDSVT
                     ELLAAAAGAVDNFAAEFERTRELRRRALRRLARATRRDNVRCDGFARVAHVTDATDWR
                     VEYPFVVICPDSEDEVAETVRACIELGLTVIPRGGGTGYSGSAVPLTRLSAVINTEKL
                     DRLGGIERIRLPGGERSADTIHCGAGVVTRRVMDAAEAAGLVFAVDPTSADASCIGGN
                     IAMNAGGKRAVLWGTALDNLVSWRMVDASGHFVEITRLDHNLGKIHDATVARFAIQRM
                     RRDARTPDGAPMILNIPGQRFRKTGLGKDVTDKFLAGLPGAQKEGCDGIVTSARFVLH
                     RMPAHVRTVCLEFFGPVREAVPSIVEIKRHCDGHPRAVLAGLEHLDERYVKAVGYATK
                     AKRGARPKMVLLADIVSDDEDAAGEAASQVVRIANLRGAEGFVAVSAEARRRFWLDRA
                     RTAAIARHTNAFKINEDVVIPLERLGDYSDGIERINIELSLRNKIALMEALEAFFGGE
                     LPVHQEAEKVAPRELLGDRVEQALGLVRGARERWQGLLDNLDAPAARLCEVSLPAAEP
                     GTRVLDALQNGSARVSWKKEVREPLLELFAGRAFEPLCRELDAVHRRVLKSRVFVALH
                     MHAGDGNVHTNIPVNSDDYAMLQEANRVVARIMRLAKSLGGVISGEHGIGITKLDYLE
                     PQEIEAFAAYKRRVDPDGCFNRGKLLPGANLDAAYTPSFRLLEQESLILEQSDIGAIS
                     DSIKDCLRCGKCKPVCSTHVPRANLLYSPRDKILAASLLIEAFLYEEQTRRGISIRHF
                     DEFGDVADHCTVCHKCASPCPVDIDFGDVSIAMRNMLRKGGRKKFNPGTAASMLFLNA
                     TDPRTVKLARKVMIDWGYRAQRAAHGAARRLGLLQAQLERPPATHGKPALRAQVIHFI
                     NKPMPG"
BASE COUNT         1019 a         2389 c         2400 g         1045 t
ORIGIN      
        1 agcgctgcgg ctggttgccg tacatgcgtg ccaggaagct gcgataggtc gccgcgcgca
       61 gcgccgcctc cacttccgcc gccagctcca gcgcgcggct cacgctccag cctgggagca
      121 ggccggcgtg caccatcgcc cagccgtact cgaagtgcat cagcgggcag tggcgcagcc
      181 aatccagcag cgtgtcgcgg tccggcgcgt ccaggacctc ctgcagcgag tcctcgcgct
      241 tgagcttctc gtagccttcg gccaccgcca gcaggtgcag atcgtggttt cccagcacca
      301 ccaccgccgc ctcgccgagg ctgcgcacga agcgcagcgt ggcgagcgaa tcgggcccgc
      361 ggttcaccag gtcgccgacc agccacaggc ggtcgctgcc ggcatcgaag ccgacctcgt
      421 cgagcaggcg ctgcagcggc tcgaagcagc cctggatgtc gccgatcgcg taggtggcca
      481 tgcggacgag aaggcgcgag cccgagaact gcgcgagacg cggcgccgcg ctgatccggt
      541 gggtcgcgca ggctacctgg cctgggcggc gagatagtcc accgccgctc ttacctccgc
      601 gtcggacagg ctgaggttgc cgcccttggc cggcatggtg cgaaagccct tgatcgcgtg
      661 ctcgtagaga gtggccttac cctgcgcgat gcgcgcctgc caggcggtct tgtcgccggt
      721 tcgcggcgca ccggcgagcc ccggcacatg gcacatggcg caggcttgct ggtggacttc
      781 ttcacccgaa cgaaccgcgg ctgcgggcgc cgtcgccgcc gcggccggtt gaacggccgc
      841 cgcgggcgcg ggcaagggcg cggcgggtgc ggctgtcgcc ggcgcctcgg cagcaccgcc
      901 cggccccagg ttgagctcgc ccaccggctt gatgcgcgcg gcgatcgcct cttccgacat
      961 gtccggcgca ccggcatcga cccgcatgcc gccggtgacg atctgcacca gcgagacgat
     1021 cagcaacacc ggcacgacga aggcgagcgc cgccagcacc gccacctgct tccagttctt
     1081 gatcggcgac gcgtgctcct ggatgtggat ctcgctcatg acggttcttg cccggcgctt
     1141 gaaaaggccc gaattatacg gccttgcccg ctgctcacca acggcggccg tggacgccgc
     1201 caatggacgc gatggcggca cagtcggtat catctgcggt ccgccgcgcc cgtagctcag
     1261 tggatagagt actggcctcc gaagccaggg gtcgtgggtt cgaatcccgc cgggcgcgcc
     1321 atgtcattcg ctcggggcga gcagcgatgc aggttttccg ccttcgcgtc atgaagccat
     1381 tgcaggtgtc gctattcgtg ctctcggcgt ggagcgtcgc cggtgcggcg ttcgcctggg
     1441 tctatccgga gcaccgggag atcgcgctgc tggctgtgcg cgacctcgac gcgtcgcgcc
     1501 gagcggtgtt cgacggctta tggcgcgagg cgcggatcac acaggagaag cgcctgtgcg
     1561 agcagggagc cgacgccgcg caaggtctgg cgccgtcgtg catcgactgg gccgcgctgt
     1621 ccgcgatcgc cggagaccat tcgtgctcga gccagcagat gaccgccacc gtcctcgagt
     1681 cggactggat cctcgtggtc gcggacgtcg cggcgcagct caagctcgac ctgtcgaaga
     1741 tcgacgtgct cccgcccgtc agccaggccc ccggaagcaa ggatctggtg acggacttgc
     1801 ggcggcgcgt gcagggcgaa gcggcgcgcg ctgcgcggat caacgcgttg cgcacctcgg
     1861 acaatcggct gcagcacgcc gatcccaagc tggcgacccg cgccgcctcc aacaacgcgc
     1921 atttccttct gccccgaccg agcaccggtg tgtccgggaa ggagtacgcg cagctcgtgc
     1981 tgaagtcggg ctccgaggtc agcgccctgg gggtctacac ctggtttcac ctgagcgcgc
     2041 tgcagaaggc gacgcggctc gccagcgagc gcgagcgcct cgcccccgag gagcgccagg
     2101 cgctcgcgcg cgccatgttg ttcgacgaag ccttcgcgct gcactttctc gaggacgtgt
     2161 tcgcggcggg gcacgtggcc gggacctggg gtgacgcatc gcagcgcaag ggcacgcacg
     2221 acttctacaa cgaggccggg ttagaggtgt ttctctggaa gggcgacggc gcgtcgatgg
     2281 tgctgatggg agacgcgcac atgcgccccg aggatgcgga gcgcgcctcg acggtggtgc
     2341 gcgcgagcct ggagcagatc ctcgacgcgg ccgccggcca ggcgcgcgac ggcagcctgc
     2401 cgtacacgag cgacgtgccc ggccaacccg acgctttcga cgtgtgcaag aacaacacga
     2461 tagcgcaacg gcccgaggcg gcgcaggtgc cggaagggta ctgggacgcc tatgccgtcg
     2521 gtctggccga agtgctgagg ccgacgccgg tcccggggct gggacccggg ctcggggcca
     2581 tgccccgctt tcgcagcgag gtggggccgt tcgtcggatt ggccgccatg ctcgacgggc
     2641 gctggttgga cgacgcgttc actgcatcgg acggcagtgg cttgatcggc ggggtcgagc
     2701 tgtccgcccg cgtcggcctc ggcctcgagg gggtgatggg agatgcgggg gacggcctgg
     2761 tatttctcgc gctcggactg cgcggcgacg cctcgtcgac caactcggtc gacgactccg
     2821 ctgcggcgaa agcgggcggc aacctcaccg ccgcgatccc ctcgcgcacc ggtatcgcca
     2881 cgcggctgcg catgcctttc tatctgatac caggcgacct gatcctgctc tctccgctcc
     2941 tgctggtggc ccccgagaaa tacgccggca tcgcggtgac ggcggcgaac ggcggtttga
     3001 ttccctggca gtcgggcatg gcgaccagga tcgggcgctt ccagttcgtc ctcggccggg
     3061 aactgggcgt caccttctac ggctacatcg gcgaggaccg cgtcatcgcg cccggcgcaa
     3121 cgccggggga acccacgcgg ctggtcgact acaagtcgac tctgttcgat ctgccgatcc
     3181 tcgagtatcg gacttaccgc tcgttcgcga gcgagcagag ctcgaccctc ctgttccaac
     3241 tgttcgcctc cgccgacgtg ccgcattcgg cgcagacggt gtccccagtg ggcgctccga
     3301 cacccgatct caagacggtg tggtccgtcg ggctccggct cctgttcgac tggcgctact
     3361 acccgtagag cgcgagttgc cgcagtgacc cgcggacgcg cgcgccaatc gcgcacaatg
     3421 cgcgcgtgca agacctggtc acgctcccca cgctgttcct gagcagcttc ctggccgcca
     3481 cgttgttgcc cggcggctcg gaggtggtgc tgctcgccgt gatcgcgcgt gagcccgcgc
     3541 tgctgtggcc cgcgctcggc tgggccacgc tgggcaacac cctcggcggg ttcagctcct
     3601 atctcctagg gcggctgctg ccgcagcgcg agaagccatc cgcagccgcg gcacgcaagg
     3661 cgcgcgactg gctgcagcgc ttcggcgcgc ccgcgctgct cctgtcgtgg gcgccggtgg
     3721 tgggcgatgc actgtgtgtg gcagcggggt ggctgcggat caacgcgctc gccgtatcgt
     3781 tgttcctggc gctgggcaag tttctcaggt actgggcggt gagccaggcg gcgctgccct
     3841 gaagggcctc atgttgcact gcggtggtgt atcatcggct gcgcagttct attcgtctct
     3901 tcaacgtctt acgcgcagat gaccggccgc cttcgggaaa tcccctacaa ctacacctcc
     3961 ttttcggatc gggagatcgt gatccgcatc ctgggcgccc gcgcctgggc gctgctcgac
     4021 gagctgcgca gccagcgccg caccggccgc tcggcgcgca tgctctacga ggtgctgggg
     4081 gacatctggg cggtggaccg caacccctat ctgcaggacg acctgctcgc caaccgccat
     4141 cgcctcgacg cgctgatcca ggcgatgcgc caccggctgc gcgagatcga ggcgcggcgg
     4201 ctcaacccgg acgacagcgt gaccgagctg ctggcggcgg cggcgggcgc ggtggacaac
     4261 ttcgccgccg agttcgagcg cacgcgcgag ctgcgccggc gggccctgcg gcggctggcg
     4321 cgcgcgacgc gccgcgacaa cgtccgctgc gacggcttcg cgcgcgtcgc gcacgtcacc
     4381 gacgccaccg actggcgcgt ggagtatccg ttcgtggtga tctgcccgga cagcgaggac
     4441 gaggttgcgg agacggtgcg tgcctgcatc gagttgggcc tcaccgtcat cccgcgcggg
     4501 ggcggcaccg gctacagcgg cagcgcggtg ccgctgacgc ggctgtccgc ggtgatcaac
     4561 accgagaagc tcgatcgcct gggcgggatc gagcgcatcc ggctgcccgg cggtgagcgc
     4621 agcgccgaca cgatccactg cggcgccggc gtggtgacgc gccgggtgat ggacgcggcc
     4681 gaggcggccg ggctggtgtt cgccgtcgac cccacctccg ccgacgcctc ctgcatcggc
     4741 ggcaacatcg ccatgaacgc gggcggcaag agggcggtgt tgtggggcac tgcgctcgac
     4801 aacctggtga gctggcggat ggtggatgcc agcggtcact tcgtggagat cacgcgcctg
     4861 gatcacaacc tgggcaagat ccacgacgcc accgtggcgc gcttcgcgat ccagcgcatg
     4921 cgcagggacg cccggacgcc cgacggtgcg ccaatgatcc tcaacatccc cgggcagcgc
     4981 tttcgcaaga ccgggctggg caaggacgtc accgacaagt tcctcgccgg gctgccgggc
     5041 gcacagaagg aaggctgcga cggcatcgtc acctcggcgc gcttcgtcct gcaccgcatg
     5101 ccggcgcatg tgcgcaccgt gtgcctggag ttcttcggtc cggtgcgcga ggcggtgccc
     5161 tcgatcgtcg agatcaagcg ccactgcgac ggccatccgc gcgcggtgct ggccgggctg
     5221 gagcacctcg acgagcgcta cgtgaaagcg gtgggctacg ccaccaaggc caagcgcggc
     5281 gcgcggccca agatggtgtt gctggccgac atcgtgagcg acgacgagga cgcggcgggg
     5341 gaagccgcct cgcaggtggt gcgcatcgcc aacctgcgcg gcgcggaggg cttcgtcgcc
     5401 gtgagcgccg aggcgcggcg ccgcttctgg ctggaccgcg cgcgcaccgc ggccatcgca
     5461 cggcacacca acgccttcaa gatcaacgaa gacgtggtga tcccgctgga gcgactcggc
     5521 gactactccg acggcatcga gcgcatcaac atcgagctct ccttgcgcaa caagatcgcc
     5581 ctgatggagg cgctcgaggc gttcttcggc ggcgagctgc cggtgcatca ggaggccgag
     5641 aaagtggcgc cgcgcgagct cctcggcgat cgcgtcgagc aggcgctggg gctggtgcgc
     5701 ggggcgcgcg agcgctggca gggactgctc gataacctgg acgcgcccgc cgcacggttg
     5761 tgcgaggttt ccttgccggc cgccgaaccg ggcacccgcg tgctcgacgc gctccagaac
     5821 ggcagcgcgc gggtgagctg gaagaaggag gtgcgcgaac cgctgctcga gttgttcgcc
     5881 ggccgcgctt tcgagccgct gtgccgtgag ctggacgccg tgcaccggcg cgtcctgaag
     5941 agccgggtgt tcgtggcgct gcacatgcac gccggcgacg gcaacgtgca caccaacatc
     6001 ccggtcaact ccgacgacta cgctatgctg caggaggcca accgggtagt ggcccgcatc
     6061 atgcgcctgg ccaagtccct gggcggggtg atctccggcg agcacggcat cggcatcacc
     6121 aagctcgact acctcgagcc gcaggagatc gaggccttcg ccgcctacaa gcggcgcgtc
     6181 gatcccgacg gctgcttcaa ccgcggcaag ctgctgccgg gcgccaacct ggacgcggcc
     6241 tacacgccga gcttcaggct gctcgagcag gagagcctga tcctggagca gagcgacatc
     6301 ggcgccatct ccgattccat caaggactgc ctgcgctgcg gcaagtgcaa gccggtgtgc
     6361 agcacgcacg tgccgcgcgc caacctgctg tacagcccgc gcgacaagat cctcgccgcg
     6421 agcctgctga tcgaggcgtt cctgtacgag gagcagaccc gccgcggcat ctcgatccgc
     6481 cacttcgacg agttcggcga cgtcgccgat cattgcaccg tgtgccacaa gtgcgcgagc
     6541 ccctgtccgg tggacatcga cttcggcgac gtgtccatcg ccatgcgcaa catgctgcgc
     6601 aagggcggcc gcaagaagtt caaccccggc accgccgcct cgatgctgtt tctcaatgcc
     6661 accgatccgc gcaccgtcaa gctcgcgcgc aaggtgatga tcgactgggg ctaccgcgcg
     6721 cagcgcgcgg cccacggcgc agcgcgccgc ctcggcctgc tgcaggcgca gctcgagcgc
     6781 ccgcccgcca cccacggcaa gcccgcgctg cgggcgcagg tcatccactt catcaacaag
     6841 ccgatgcccg gtg
//
LOCUS       DASPGE010000187         7140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_636366, whole genome shotgun sequence.
ACCESSION   DASPGE010000187 DASPGE010000000
VERSION     DASPGE010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636366"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..770
                     /locus_tag="VD791_05945"
     CDS             72..770
                     /locus_tag="VD791_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXV07550.1"
                     /translation="MLDKLIELSFLSGGLLPLMAVLLLVTLAVIVERLYFYHRVLQSG
                     LAMEHDLQLVEYQNVDKLEKLAAHYDGSLQRSIISSALASRGQDAETMERHIDEAIMW
                     QMPKIDKHVWVLDSSVTLAPLMGLLGTIIGMVHAFGILGTSGAGGAASQMVTGGIAEA
                     LVATGVGLLIAIVAVAFLNYFNKRGRLALHQMEQLKVMVINRLHGGGSAHHGVPVREA
                     HAGKVTPISAGRRP"
     gene            770..1192
                     /locus_tag="VD791_05950"
     CDS             770..1192
                     /locus_tag="VD791_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXV07551.1"
                     /translation="MRSGRSGYFDAQRPRLEVVPMIDIMMFLLVFFVIVAIKMIAGTG
                     VEMELPGSTTTRELKSSVVTVGVTKSGETVFDGKSVTAAELRDRLRALKQDKPIDVVL
                     AGDKDASLQTLLAVMDSVRSAGISSVGLAAKAEESPQK"
     gene            1189..1884
                     /locus_tag="VD791_05955"
     CDS             1189..1884
                     /locus_tag="VD791_05955"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXV07552.1"
                     /translation="MIPEARRARGPLWAQVEEDDEQGLPLWKALLIAAALELLLPVLL
                     FGVDWSFLGWSEPPPIPVMSVRLEPPPETPPEPPEPDKRKPAPEKPIRQVEIELPPPL
                     PDAPPPNIVLPKPEPKPEPQAKREEEPPPPQPQPEPEAPPLPSVFRDVKPVKKVKPVY
                     PREAEDQHIQGRVRVRLSVSLEGDVTDVEVLLAEPPGVFDEAVLTAVRQYKFKRDGTR
                     YQADQEIVFRIDD"
     gene            complement(1906..2454)
                     /locus_tag="VD791_05960"
     CDS             complement(1906..2454)
                     /locus_tag="VD791_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07553.1"
                     /translation="MQALEPLARLWRGLYRPWSRRTRDRPLVGLGLARMPAAPPADWV
                     GEAAAARSRASEARARKLLCRMLSTSQLKELETHGCFTVEVTGRGRFCILPRTTFNVF
                     HVQSGNLYCCNTEAFVPLSDLMLAQKLLLERDPDRFFAVANCRPEVFAGPWEQQLRRA
                     LNREQERAPRRGQAPNFHHSLR"
     gene            complement(2527..2760)
                     /locus_tag="VD791_05965"
     CDS             complement(2527..2760)
                     /locus_tag="VD791_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07554.1"
                     /translation="MKTLRILDSSGDRVLTFDDSAASAKARREAEALFTRMLGNGATA
                     FKVNRGEGKSDEKVTDFSALENETIVVPRVVGG"
     gene            complement(2832..3476)
                     /locus_tag="VD791_05970"
     CDS             complement(2832..3476)
                     /locus_tag="VD791_05970"
                     /inference="COORDINATES: protein motif:HMM:NF021039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HXV07555.1"
                     /translation="MARLRHLLAVGLVAGVAAGAAATLLQAATVVPLILQAEALEPHH
                     AAEHSVAARSAATWLFNSLAGIGFGLLLAAGYNLSRRAGLRTGLLWGLGGFVAFYLAP
                     ALGLPPSLPGTELAGLGARQVWWLATAVATATGLTLLVFGSRLWMRGCGVVLVALPHL
                     AGAPQPDTVAVGLPQHLVRAFAVWSAVGNALFWLVLGAVSGWLFQRFTMASARR"
     gene            complement(3487..3672)
                     /locus_tag="VD791_05975"
     CDS             complement(3487..3672)
                     /locus_tag="VD791_05975"
                     /inference="COORDINATES: protein motif:HMM:NF021038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB domain-containing protein"
                     /protein_id="HXV07556.1"
                     /translation="MPDSRIPSHAPAAAPALVRAWQTTLVALLGAAIVLGVGFAHLPQ
                     VHDAAHDTRHAFNFPCH"
     gene            complement(3711..4505)
                     /locus_tag="VD791_05980"
     CDS             complement(3711..4505)
                     /locus_tag="VD791_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HXV07557.1"
                     /translation="MTAKARSLQPVPGSAPVRERVSREEWETRCDLAAAYQLAALFRW
                     TDLIYTHFSARVPGTDDLLINPYGLMFHEITASTLVKIDMQGNVLDDPSGLGMNLAGF
                     VIHGCVHQAREDVKCVIHTHTRAGVAVSAQKQGLLPLSQHAVRVYATLSYHDYEGLAF
                     DLDERTRLARDLGPTSKAMILRNHGLLTVGATVPEAFELMYYLDCACQVQVDALANGR
                     AEVLLLSEEAARKGFEQFEHSPQPTLPKQWAALLRMLERKGIDYRA"
     gene            4663..5076
                     /locus_tag="VD791_05985"
     CDS             4663..5076
                     /locus_tag="VD791_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV07558.1"
                     /translation="MKIRSFAESARPQATSLAWLLAAAVAGSLLAPAAQAEGNAAMGR
                     TLYERRCLGCHGDANTPNAQGPSLVGVVGRKAGTAGGGATSRALSESGMIWDEGTLGQ
                     FLAAPSDTVHGTIMPVGTARPQDREDLIAYLKTLQ"
     gene            5158..6039
                     /locus_tag="VD791_05990"
     CDS             5158..6039
                     /locus_tag="VD791_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXV07559.1"
                     /translation="MNARFVNALLRHPNRYPSHLEARHPGILERIAQLWGTSQLDSYL
                     QELLLDTTRGAHQGFAPAVMSDLMFLHGLHLECKQETPVPVSDVWGVERVRKGLVQEK
                     WSDPQFMLDRAVRDGNEVAVRLFLRDGADVRRRNASGWTPLMVASFTGNRRMAAILVE
                     ADADVNAQDARGYAPLHWAAFKNYHEVVQLLLQRGAFVNIKSASGLTPLLQASAQGAL
                     QSMKLLLRAGAEVNEPDNEGWTPLHKAIANDHLKAVELLVEEGANWQATHRSGVTPVA
                     LARRKSHMAYLVARAIA"
     gene            complement(6121..>7140)
                     /locus_tag="VD791_05995"
     CDS             complement(6121..>7140)
                     /locus_tag="VD791_05995"
                     /inference="COORDINATES: protein motif:HMM:NF023407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HXV07560.1"
                     /translation="EAAEEAAWRRHLAVYPWVRGALGMDAFARCWVQEDALLGAVGAL
                     LPVAQHDAFARLRAAWRARNVEVFEQSMQMLARQLATVAVDREAVPVPNLKDTATRLL
                     STLLSSGEKADPEAERAMTALATRGDAAEREATDRLIALHELSGRAAEQILARVSGQF
                     EVSRPADVGKSGVVGGLVSGALGGLAADLAAGGLTFGAGALIGGLLGALGVGGATKAY
                     NLMLGTSEGQIAWSAEFLTQRVSAALLRYLAVAHFGRGRGDWVESEYPAHWQALVEEA
                     TAERRAQLDDIWRRAEAGAAREEIEALLQPLVARAARVLLVRLYPEAREVLRDTTLPR
                     SPDGA"
BASE COUNT         1162 a         2532 c         2442 g         1004 t
ORIGIN      
        1 cgccggcacc ccgcctcgtg agaggcgggg agtgattttc tttgaatcgc gccttcaggg
       61 cgcctaaagc catgctagac aaactgatcg aactgagctt cctctccggg ggcctgctgc
      121 cgctgatggc ggtgctgctg ctggtcacgc tggcggtgat cgtcgagcgg ctgtacttct
      181 accatcgcgt gctgcagtcc gggctcgcca tggagcacga cctgcaactg gtcgagtacc
      241 agaacgtgga caagctcgag aagctcgccg cgcactacga cggatcgctg cagcgctcga
      301 tcatctcctc ggcgctcgcc tcgcgcggcc aggacgcgga gaccatggag cgccacatcg
      361 acgaggcgat catgtggcag atgcccaaga tcgacaagca cgtgtgggtg ctggacagct
      421 ccgtgaccct ggccccgctg atgggcctgc tcggcaccat catcggcatg gtgcatgcct
      481 tcggcatcct gggcacctcg ggtgccggcg gcgccgcgtc gcagatggtc accggcggca
      541 tcgccgaggc gctggtcgcc accggcgtgg gcctgctgat cgccatcgtc gcggtggcct
      601 tcctgaacta tttcaacaag cgcgggcggc tggcgctgca ccagatggag cagctcaagg
      661 tgatggtgat caaccgcctg cacggcggcg gctccgcgca ccacggcgtg ccggtgcgcg
      721 aggcgcacgc cggcaaggtg acgccgatca gcgccggcag gaggccgtga tgcgcagcgg
      781 ccgcagcggt tacttcgacg cgcagaggcc gcgcctcgaa gtcgtgccga tgatcgacat
      841 catgatgttc ctgctggtgt tcttcgtgat cgtcgcgatc aagatgatcg ccggcaccgg
      901 cgtggagatg gagctgcccg gctcgaccac cacccgcgag ctgaagagct ccgtcgtcac
      961 cgtgggggtg acgaaatccg gcgagacggt gttcgacggc aagtccgtca ccgccgccga
     1021 gctgcgcgac cggctgcgcg cgctgaagca ggacaagccg atcgacgtgg tgctggcggg
     1081 cgacaaggac gcttccctgc agacgctgct ggcggtgatg gacagcgtgc gcagcgccgg
     1141 catcagttcc gtgggcctgg cggccaaggc ggaagaatcc ccgcagaaat gatacccgag
     1201 gcgcgccgcg cgcgcggccc gctgtgggcg caggtcgaag aggacgacga gcaggggctg
     1261 ccgctgtgga aggcgttgct gatcgcggcc gccctcgagc tgcttctgcc ggtgctgctg
     1321 ttcggcgtgg actggtcgtt cctcggctgg agcgagccgc cgccgatccc ggtgatgtcg
     1381 gtgcggctgg agccgccgcc ggagacgccg cccgagccgc ccgaaccgga caagcgcaag
     1441 ccagcacccg agaaaccgat ccggcaagtc gagatcgagc tgccgccgcc gctgcccgac
     1501 gcgcccccgc cgaacatcgt gctgccgaaa cccgaaccga agcccgagcc gcaggcgaag
     1561 cgggaggagg agccgcctcc cccgcagccg caacccgagc ccgaggcgcc gccgctgccg
     1621 tcggtgttcc gcgacgtgaa gccggtgaag aaggtcaagc cggtgtatcc gcgcgaggcc
     1681 gaagaccagc acatccaggg ccgcgtcagg gtgcgcctgt cggtcagcct ggagggcgac
     1741 gtcaccgacg tcgaagtgct gctggcggaa ccgccgggcg tcttcgacga agcggtgctg
     1801 acggcggtgc ggcaatacaa gttcaagcgc gacggcacgc gctaccaggc cgaccaggag
     1861 atcgtgttca ggatcgacga ctagcgggcc cgggccgttt ccaggctagc gcaaggaatg
     1921 gtggaagttc ggagcctgcc cgcgccgcgg cgcgcgttcc tgctcgcggt tgagcgcgcg
     1981 ccgcagctgc tgctcccagg gcccggcgaa cacctcgggc cggcagttgg ccaccgcgaa
     2041 gaaccggtcg ggatcgcgct cgagcagcag cttctgcgcg agcatcaggt cggacaacgg
     2101 cacgaacgcc tcggtgttgc agcagtagag gttgccgctc tgcacgtgga acacattgaa
     2161 ggtggtgcgc ggcaggatgc agaagcgccc gcgcccggtc acctccacgg tgaagcagcc
     2221 gtgcgtctcc agctccttga gctgcgacgt cgacagcatg cggcacagca gcttgcgcgc
     2281 gcgcgcctcg gaagcgcgcg agcgcgcagc cgccgcctcg ccgacccaat cggcgggcgg
     2341 agcggccggc atccgcgcca ggccgaggcc gaccaacggg cggtcgcggg tgcgccgcga
     2401 ccacggccgg tacaggcctc gccacaagcg cgccaacggc tccagagctt gcatgcgctg
     2461 tccttcgcgc cgctcggggc ggcgacctgc cgggcccgcc tggcgggcgg cgcgagaagt
     2521 gccgctctag ccgccgacca cgcgcggaac gacgatcgtc tcgttctcca acgccgagaa
     2581 gtcggtgacc ttctcgtcgg acttgccctc tccgcgattc accttgaacg cagtcgcgcc
     2641 gttgcccagc atgcgcgtga acagcgcttc ggcttcgcgc cgcgccttgg cgctggccgc
     2701 gctgtcgtcg aacgtcaaca cgcggtcgcc cgaggaatcg agaatgcgaa gggtcttcat
     2761 accgcctcct gtcgtgatgc caaacctctg gttcgacctc gggtcggcga aggtcgttcc
     2821 ctccccgatg gctagcgccg cgcgctcgcc atcgtgaagc gttggaacag ccagccggaa
     2881 accgccccga gaaccagcca gaacaacgcg ttgcccacag cggaccagac cgcgaacgcg
     2941 cgcaccaagt gctgcggcag tcccacggcc acggtatccg gttgcggcgc accggccaga
     3001 tgcggcagcg ccacaagcac caccccgcac ccgcgcatcc aaagcctgct cccgaacacc
     3061 agcaacgtca agccggtcgc ggtggccacc gcggtggcga gccaccagac ctggcgtgcg
     3121 cccagtccgg ccagctcggt gccgggaagg ctgggcggca atcccagtgc aggcgcgaga
     3181 tagaaggcga cgaacccgcc cagcccccac agcagcccgg tgcgcagccc cgcgcgccgg
     3241 gacaggttgt agcctgcggc gagcagcagc ccgaagccga tgccggcaag cgaattgaac
     3301 agccaagtgg cggcgctgcg tgccgccacc gagtgttctg cggcgtggtg cggctccagc
     3361 gcctcggcct ggaggatcag cggaacgacg gtggccgcct gcagcagcgt ggcggccgcc
     3421 ccggcggcca cgccggcgac caatcccacc gcgagcaaat gccgcagccg cgccacggcg
     3481 gcgccctcaa tgacagggaa agttgaaggc gtgccgcgtg tcgtgcgccg cgtcgtgcac
     3541 ctgcggcaga tgggcgaaac ccacgcccag cacgatcgcc gcgcccagca gcgcgaccag
     3601 cgtcgtctgc caggcgcgga ccagggcggg ggcggccgcg ggtgcgtggc tcggaatgcg
     3661 cgagtccggc atcggagttc ccagggacat gaatctcagg gggacatgga tcaggcgcga
     3721 tagtcgatgc ccttgcgctc gagcatgcgc agcagcgccg cccactgctt gggcagcgtg
     3781 ggctgaggcg aatgctcgaa ctgctcgaag cccttgcgcg cggcctcctc ggagaggagc
     3841 agcacctcgg cgcggccgtt ggccagcgca tcgacctgga cctggcaggc gcaatccagg
     3901 tagtacatca gctcgaacgc ttcgggcacc gtcgcgccca ccgtgagcag gccgtggttg
     3961 cgcaggatca tcgccttgga cgtggggccg aggtcgcgcg ccagccgcgt gcgctcgtcc
     4021 aggtcgaagg cgaggccctc gtagtcgtga taggagagcg tggcatacac gcgcacggca
     4081 tgctggctga ggggcagcag cccctgcttc tgcgccgaca ccgccacgcc ggcgcgcgtg
     4141 tgggtgtgga tcacgcactt cacgtcctcc cgcgcctggt gcacgcagcc gtggatcacg
     4201 aacccggcca ggttcatgcc cagcccgctg gggtcgtcga gcacgttgcc ctgcatgtcg
     4261 atcttcacca gcgtggaggc ggtgatctcg tggaacatca ggccgtaggg gttgatcagc
     4321 agatcgtcgg tgccgggcac gcgcgccgag aagtgcgtgt agatcaggtc ggtccagcgg
     4381 aacagggccg cgagctggta agccgccgcc aggtcgcagc gcgtctccca ctcctcgcgg
     4441 ctgacgcgct cgcgcaccgg ggcgctgccg ggtacgggct gaagcgatct cgctttggcg
     4501 gtcatgcggt tctccctggg gtggcacgtg gctgccgaaa taatacgccc gatcgccgcc
     4561 ccgacgcagg cgcctcgtcc cgctgcgctt gacccaaatc aagcgctgcc gcaagccgcg
     4621 cgcttagcgt gccttggtat actcatcacc agggaggaag cgatgaaaat ccggtcattc
     4681 gccgaatcag cccggccgca ggcgacatcc ctcgcgtggt tgctggccgc ggcggtcgcg
     4741 gggagcctgc tggcgccggc ggcgcaagcc gaaggcaatg cggccatggg caggaccctt
     4801 tatgagcggc gttgcctggg ttgccatggc gacgccaaca ccccgaatgc ccagggcccg
     4861 agcctggtcg gtgtggtcgg acgcaaggcg ggcaccgccg gcggcggcgc cacttcgcgc
     4921 gcgctcagcg agtccggcat gatctgggac gaaggcacgt tggggcaatt tctggccgcg
     4981 ccctcggaca cggtgcacgg caccatcatg ccggtcggca ccgctcgccc ccaggaccgc
     5041 gaggacctga tcgcctacct caagaccctg caatagcgcc tggccgcgtt cttgcttggc
     5101 agccggcgat ttcgcaaatc cgacaagaac ccgttaccgt cgcctgacat ttcgcccatg
     5161 aacgcccgct tcgtcaacgc cctgctgcgt catcccaacc gctatccgag ccacctggag
     5221 gcgcgccacc cgggcatcct ggagcgcatc gcccaactct gggggaccag ccagctcgac
     5281 agctacctgc aagagctgct gctcgacacc acccggggcg cccaccaggg gttcgcgccc
     5341 gcggtgatga gcgacctgat gttcctgcac ggcttgcacc tcgagtgcaa acaagaaacg
     5401 ccggtaccgg tgagcgacgt gtggggcgtc gagcgcgtgc gcaaggggct ggtgcaggag
     5461 aaatggtccg atccacagtt catgctggat cgcgcagtgc gcgacggcaa cgaggtcgct
     5521 gtacgcctgt tcctgcgcga cggcgccgac gtgaggcgcc gcaacgcgag cggctggacg
     5581 cccttgatgg tcgccagctt caccggcaac cgccgcatgg ccgcgattct ggtcgaggca
     5641 gacgcggacg tgaacgccca ggacgcacgc gggtacgcac cgctgcactg ggccgccttc
     5701 aagaactatc acgaggtggt gcaactgctg ctgcagcgcg gcgccttcgt caacatcaag
     5761 agcgcgtccg gcctcacgcc gctgctgcag gcctccgcgc aaggcgcgct gcagtcgatg
     5821 aagctgctgc tgcgcgccgg ggccgaagtc aacgagccgg acaacgaggg ctggacgccg
     5881 ctgcacaagg cgatcgccaa cgaccacctc aaggcggtgg agctgctcgt cgaggaaggc
     5941 gccaactggc aagcgacgca ccgctcgggc gtgacgccgg tggcgctcgc ccgccgcaag
     6001 tcgcacatgg cctatctcgt ggcgcgcgcc atcgcctgac gagcggtagc ccgcagcgtg
     6061 tagcccggat gaagcgcagc gtaatccggg gaacaaaagg tctttcgccc ccggattccg
     6121 ctacgctcca tccggactac gcggcaacgt tgtatcgcgc aatacttccc tcgcctccgg
     6181 atacagccgc accagcagca cgcgcgccgc gcgggcgacc agcggctgca gcagcgcctc
     6241 gatctcctcg cgcgcagcgc ccgcttccgc cctgcgccag atgtcgtcca gctgcgcacg
     6301 ccgttccgcg gtcgcttcct ccaccagcgc ttgccagtgc gcggggtact cgctttccac
     6361 ccagtcgccg cggccgcggc cgaagtgcgc cacggcaagg tagcgcagca acgccgccga
     6421 cacgcgttgc gtgaggaact ccgccgacca ggcgatctgt ccctcgcttg tgccgagcat
     6481 gaggttgtag gccttcgtcg cgccacccac ccccagggcg ccgagcaaac cgccgatcaa
     6541 cgcgcccgcg ccgaaggtca gcccgcccgc ggccaggtcg gcggccagtc cgccgagcgc
     6601 gcccgacacc agtccgccga cgacgcccga cttgcccacg tccgcagggc gcgacacctc
     6661 gaactgtccc gacacgcgcg ccaggatctg ctccgccgcg cgtcccgaca gctcgtggag
     6721 cgcgatcagc cgatcggtcg cctcgcgctc cgccgcgtct ccccgcgtgg caagcgcggt
     6781 cattgcgcgt tcggcttcgg gatcggcctt ctcgccgctg ctcaacagcg tcgagagcaa
     6841 gcgggtcgcg gtgtctttca ggttgggtac cggcacggct tcgcggtcga cggcgaccgt
     6901 ggcgagctgc cgcgcgagca tctgcatcga ctgctcgaac acctccacgt tgcgcgcgcg
     6961 ccatgccgcg cgcaagcgcg cgaaggcgtc gtgctgcgcc actggcagca gcgcaccgac
     7021 ggcgccgagc agcgcgtcct cttgcaccca gcagcgcgcg aaggcgtcca tccccagcgc
     7081 gccgcgcacc caggggtaca ccgccaggtg ccggcgccat gccgcctcct cggccgcctc
//
LOCUS       DASPGE010000188         3196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_871116, whole genome shotgun sequence.
ACCESSION   DASPGE010000188 DASPGE010000000
VERSION     DASPGE010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871116"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VD791_06000"
     CDS             <1..741
                     /locus_tag="VD791_06000"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HXV07561.1"
                     /translation="LLAFAAGEVAGAPAERLEIAAAAVELIHAYSLVHDDLPCMDDDV
                     LRRGRPTCHVAYDEATALLVGDALQSLAFQVLAEQPLAADAGTQLEMVRTLAVAVGSR
                     GMAGGQAIDLASTGAELTLPELENMHIHKTGELIRAAILLGARCGNRLPPAGIEALDR
                     FSKCAGLAFQVVDDVLDAESDTDTLGKTAGKDARQNKPTYVSTLGLSQARGLAKELRN
                     DALAALDAFGSEAQRLRELIDFIVLRSR"
     gene            764..2608
                     /gene="dxs"
                     /locus_tag="VD791_06005"
     CDS             764..2608
                     /gene="dxs"
                     /locus_tag="VD791_06005"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HXV07562.1"
                     /translation="MYELLESINDPAELRKLDRKELSQLAAELRQYLLESVARTGGHL
                     SSNLGTVELTVALHYVFDTPNDRLVWDVGHQTYAHKILTGRRQAMSGLRTYGGIAGFP
                     RREESAYDTFGTAHSSTSIAAALGMAVAARQAGEERRVVAVIGDGAMTAGMAFEALNN
                     AGALDVNLLVILNDNDMSISENVGALNNYLARLMSGRFYTAARRAGQRMLSALPPVLD
                     FARRAEEHVKGMVTPGTLFEEFGFNYIGPIDGHDLDVLVTTLTNIRQLERPQFLHVVT
                     RKGKGYKPAEEDPILYHGVTRFDPQAGIQSKPPGKPTYTQVFGDWLCDMAAQDARLIA
                     ITPAMREGSGLVRFSQEFPERYFDVGIAEQHALTFAAGLACEGLKPVVAIYSTFLQRG
                     YDQLIHDVAIQSLPVMLAIDRGGLVGADGPTHHGAFDLTYLRCIPNLVVMTPSDENEC
                     RQMLYTAYRLDQPAAVRYPRGSGPGTPIEPRMQALALGKGEMRRQGRRIALLAFGSMV
                     KPAMEAAAQLDASVADMRFVKPLDLDLVLRLASSHELLVSVEENVIEGGAGSAVAEAL
                     SAAGRAVPLLQLGLPDRFVEHGDPAVLLKACGLDRDGILAAIGRRLPE"
     gene            2662..>3196
                     /locus_tag="VD791_06010"
     CDS             2662..>3196
                     /locus_tag="VD791_06010"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HXV07563.1"
                     /translation="MNDVRAFAIPDVQSTADTRRLAIDRVGIKGIRHPARVRDKSGGV
                     QHTVAQFNMYVHLPHNFKGTHMSRFVEILNSHEREISVESFETILREMVERLDAECGR
                     IEMSFPYFINKAAPVSGVESLMDYEVTFVGEIEKGRYRFTMRVVVPVTSLCPCSKDIS
                     DYGAHNQRSHVTVTARTR"
BASE COUNT          522 a         1102 c         1106 g          466 t
ORIGIN      
        1 ctgctcgcct tcgcggccgg cgaggtcgcc ggcgcgccgg ccgagcgcct ggagatcgcc
       61 gccgccgcgg tcgagctcat ccacgcctac tcgctcgtgc acgacgatct gccctgcatg
      121 gacgacgacg tgctgcgccg cggccgcccc acctgtcacg tcgcatacga cgaggccacc
      181 gcgctgctgg tcggcgatgc gctgcagagc ctggcgttcc aggtgctggc ggagcagccg
      241 ctcgctgcgg acgccggcac ccagctcgag atggtgcgca cgctcgcggt cgcggtgggc
      301 tcgcgcggca tggccggcgg gcaggccatc gacctggcca gcaccggtgc ggagttgacg
      361 ctgccggagc tggagaacat gcatatccac aagaccggcg agctgatccg cgccgccatc
      421 ctgctcggcg cgcgctgcgg aaaccgcctg cccccggcgg gcatcgaagc tctggaccgg
      481 ttctcgaagt gcgccgggtt ggccttccag gtggtggacg acgtcctcga cgccgaatcg
      541 gacaccgaca cgctgggcaa gacggccggc aaggatgccc ggcagaacaa gcccacctac
      601 gtgagcacgc tgggcctgtc gcaggcgcgc gggctcgcga aggagctgcg caacgacgcg
      661 ctggcggcgc tcgacgcctt cggcagcgag gcgcagcgcc tgcgcgagct gatcgacttc
      721 atcgtgttgc gcagccgcta gggaagctct gaacaagcat cgcatgtacg agctgctcga
      781 aagcatcaac gatcccgccg agctgcgcaa gctcgaccgc aaggagctgt cgcagctcgc
      841 cgccgagctg cgccagtacc tgctcgagtc ggtggcgcgc accggcggcc atctgtcctc
      901 gaacctcggc accgtcgagt tgacggtggc gctgcattac gtgttcgaca cgccgaacga
      961 tcggctggtc tgggacgtcg gccaccagac ctacgcgcac aagatcctca ccggccggcg
     1021 ccaggccatg tcggggctgc gcacctacgg cggcatcgcc ggcttcccgc ggcgcgagga
     1081 gagcgcctac gacaccttcg gcacggcgca ctccagcacc tcgatcgccg cggcgctggg
     1141 catggcggtg gcggcgcggc aagcgggcga ggagcgccgc gtggtggcgg tgatcggcga
     1201 cggtgcgatg accgccggga tggcgttcga ggcgctgaac aacgccggcg ccctcgacgt
     1261 caacctgctg gtgatcctca acgacaacga catgtcgatc tcggagaacg tcggggcgtt
     1321 gaacaactac ctggcgcgcc tcatgtccgg gcgcttctac acggccgcgc gccgcgccgg
     1381 gcagcgcatg ctctcggcgc tgccgccggt gctggacttc gcgcgccggg ccgaggagca
     1441 cgtgaagggc atggtcaccc cggggacgct gttcgaggag ttcggtttca actacatcgg
     1501 cccgatcgac ggtcacgacc tggacgtgct ggtgaccacg ctcaccaaca tccgccagct
     1561 cgagaggccg cagttcctgc acgtggtcac gcgcaagggc aagggctaca agccggccga
     1621 agaggacccg atcctgtatc acggcgtgac ccgcttcgac ccgcaggccg gcatccagtc
     1681 gaagccgccc ggcaagccga cctacaccca ggtgttcggc gactggctgt gcgatatggc
     1741 tgcgcaggac gcgcgcctga tcgcgatcac cccggcgatg cgcgagggct ccgggctggt
     1801 gcgcttctcc caggagttcc cggagcgcta tttcgacgtc ggcatcgccg agcagcacgc
     1861 gctgaccttc gccgccggcc tggcgtgcga aggattgaaa ccggtggtgg cgatctattc
     1921 gaccttcctg cagcgcggct acgatcagct gattcacgac gtggcgatcc agagcctgcc
     1981 ggtcatgctg gcgatcgacc gcggcgggct ggtgggagcg gacggcccca cgcatcacgg
     2041 tgccttcgac ctcacctacc tgcgctgcat ccccaacctg gtggtgatga cgcccagcga
     2101 cgagaacgaa tgccggcaga tgctctacac cgcctatcgc ctggaccagc cggcggcggt
     2161 gcgctacccg cgcggcagcg gacccgggac gcccatcgag ccgcgcatgc aggcgctggc
     2221 gctgggcaag ggcgagatgc gcaggcaggg ccgcaggatc gcgctgctgg ccttcggcag
     2281 catggtgaag cccgccatgg aggccgccgc gcagctcgac gccagcgtcg ccgacatgcg
     2341 cttcgtgaag cccctggacc tggacctggt gcttcggctc gccagctcgc acgagctgct
     2401 ggtgagcgtc gaggaaaacg tgatcgaggg cggagcgggc agcgccgtgg ccgaggcctt
     2461 gagcgcggcc ggcagggcgg tgcccctgct ccagctcggg ctgcccgacc gcttcgtcga
     2521 gcatggcgac cccgccgtgc tgctcaaggc gtgcggcctg gaccgcgatg gcatcctggc
     2581 cgccatcggc cgccgtttac ccgagtagtt tagtcgggta aggtgcgcta taatgcgcca
     2641 acagccaacc ccaggaagaa catgaacgac gttcgcgctt tcgcaatccc cgacgtgcag
     2701 agcacggccg acacgcgccg gctggcgatc gaccgcgtgg ggatcaaggg catccgccat
     2761 cccgcgcgcg tgcgcgacaa gagcggcggg gtgcagcaca ccgtggcgca gttcaacatg
     2821 tacgtgcatc tgccgcacaa cttcaagggc acgcacatgt cgcgcttcgt ggagatcctc
     2881 aacagccacg agcgcgagat ctcggtggag tcgttcgaga ccatcctgcg cgagatggtg
     2941 gagcggctgg acgcggagtg cgggcgcatc gagatgagct ttccgtactt catcaacaag
     3001 gccgcaccgg tgtccggcgt ggaaagcctg atggactacg aggtcacctt cgtcggcgag
     3061 atcgagaagg gccgctaccg gttcaccatg cgggtggtgg tgccggtgac cagcctgtgc
     3121 ccctgctcca aggacatctc cgactacggc gcgcacaacc agcgctcgca cgtgacggtg
     3181 accgcgcgca cgcgcg
//
LOCUS       DASPGE010000189         3173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_51861, whole genome shotgun sequence.
ACCESSION   DASPGE010000189 DASPGE010000000
VERSION     DASPGE010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51861"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="VD791_06015"
     CDS             <1..1367
                     /locus_tag="VD791_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXV07564.1"
                     /translation="TVGVAALGGLLLWEAQDAKTGWYVLGGAVGTMAVLALVTLALLR
                     LATWSGRRAPFAWRFGLANLQRRRFSTVTQVVALGVGIMALVLLTLVRSDLLANWRQS
                     LPADAPNRFLVNVQPDQVEPLRAFFAQHGFAAPQMFPMVRGRLVAINDRPVSSLDYSE
                     DRAKRLIDREFNLSWAARPQVDNVIVAGRWWTPAQRDAASFSAEDGLSSVDDGSLSVE
                     EGLFSVEDGIAQTLGIRMHDRLTYEIAGSRLEGTVSSLRKVEWDSFRVNFFVVTPPGM
                     LQGYPASYVTSFHLPAERGALMDRLVQSFPNVLVIDVASVLAQVQRIMDQLVKAVEFV
                     FLFGLLAGLVVLFAAISATHDERVFDAAILRTVGATGRQMLSAQAAEFLAIGALAGLL
                     AAIGATLLGYVLATRVLNVPYALDPWVWLAGLAGGTGGVLLAGLAGTRRILRTPPMEI
                     FRRL"
     gene            1389..1772
                     /locus_tag="VD791_06020"
     CDS             1389..1772
                     /locus_tag="VD791_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HXV07565.1"
                     /translation="MSETLSREKNVLGGPLRACSFDPLTGYFRDGCCGTRGERGVAHL
                     VCVRVSAEFLAFSREHGNDLTTPRPEMRFRGLNPGDRWCLHVLRWVEAWEAGKAPPVV
                     LEATHENVLQFAPLTALKRHALNVS"
     gene            1926..>3173
                     /locus_tag="VD791_06025"
     CDS             1926..>3173
                     /locus_tag="VD791_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXV07566.1"
                     /translation="MSSTVRNIAIIAHVDHGKTTLVDKLLRQSGVFAAHQHVEERVMD
                     SNALERERGITILAKNAAFDYRGVHINIVDTPGHRDFGGEVERALSMVDGVLVLVDAV
                     EGPMSQTRFVTRKALARGLRPIVVVNKIDRPGARADWVVNRTFDLFDKLGATEEQLDF
                     PVVYASALQGVATLDLAQPGQDLRPLFETILSRVPAPGGDPDMPLQLQISALDYSSYI
                     GRIGIGRIRQGRLRAGQEVLIFGGAPHSSSTTGVEPQKARVTQVYGFQGLQRAPLQEA
                     QAGDIVLLQGIEELTIGCTLCDPEDPHPLPLLAVDEPTLTMNFQVNTSPFAGREGKFV
                     TSRQLRERLQRETLSNVALRVQDTADADVFRVSGRGELHLTILLENMRREGYELAVSK
                     PRVVFKEVDGQRQEPMELVTADVE"
BASE COUNT          440 a         1078 c         1145 g          510 t
ORIGIN      
        1 acacggtggg cgtggccgcg ctgggcggtc tgctgctgtg ggaagcgcag gacgcgaaga
       61 ccggctggta cgtgctcggc ggcgcggtgg gcacgatggc cgtgctggcg ctggtcacgc
      121 tggccttgct gcggttggcg acgtggtcgg ggcggcgcgc accgttcgcc tggcgcttcg
      181 ggctcgccaa cctgcagcgc cggcgcttca gcaccgtgac ccaggtggtc gcgctcggcg
      241 tcggcatcat ggcgctggtg ctgctcaccc tggtgcgcag cgacctgctc gccaactggc
      301 ggcagagcct gcccgccgat gcgcccaacc gcttcctggt caacgtccag ccggaccagg
      361 tggagccgct gcgcgccttc ttcgcgcagc acggcttcgc cgcgccgcag atgttcccca
      421 tggtgcgcgg gcggctggtg gcgatcaacg atcggccggt gtcctccctg gattacagcg
      481 aggaccgcgc caagcgcctc atcgaccgcg agttcaacct gtcgtgggcg gcgcggccgc
      541 aagtggacaa cgtgatcgtg gccggccgct ggtggacgcc ggcgcagcgc gacgccgcat
      601 cattctcggc cgaggacggg ctgtcctcgg tcgacgacgg ctcgttgtcg gtcgaggagg
      661 ggctgttctc ggtcgaggac ggcatcgcgc agacgctcgg catccgcatg cacgaccggc
      721 tgacctacga gatcgccggc agccgcctgg agggcacggt cagctcgctg cgcaaggtgg
      781 aatgggattc cttccgcgtg aacttcttcg tggtgacgcc gccgggcatg ctgcagggct
      841 acccggcgag ctacgtcacc agcttccacc tgcccgccga gcgcggcgcg ctgatggacc
      901 ggctggtgca gagctttccc aacgtgctgg tgatcgacgt cgcatcggtg ctggcgcagg
      961 tccagcgcat catggatcag ctggtgaagg cggtggagtt cgtgttcctg ttcgggctgc
     1021 tggccgggct ggtggtgctg ttcgccgcga tcagcgcgac gcatgacgag cgcgtgttcg
     1081 acgccgccat cctgcgcacc gtcggggcca ccggccgcca gatgctgagc gcgcaggccg
     1141 cggagttcct ggcgatcggc gcgctggccg gcctgctcgc cgcgatcggc gcgaccctgc
     1201 tgggatacgt gctcgccacg cgcgtgctca acgtccccta cgccctcgac ccgtgggtct
     1261 ggctcgcggg gcttgccggg ggcacgggcg gcgtgctcct tgccgggctg gccgggacgc
     1321 ggcgcatcct gcgcacgccg ccgatggaga tcttccgcag gctctgaggg ttcggccata
     1381 atgccggcgt gagcgagacc ctgtcccggg aaaagaacgt gctcggcgga ccgctgcggg
     1441 cctgcagctt cgatccgctc accgggtatt tccgcgacgg atgctgcggg acgcgcgggg
     1501 agcgcggcgt tgcccacctg gtgtgcgtgc gggtaagcgc cgagttcctg gctttctccc
     1561 gcgagcacgg caacgacctc accaccccgc gcccggagat gcgcttccgg gggctcaacc
     1621 ccggcgaccg ctggtgcctg cacgtgctgc gctgggtgga agcgtgggag gcgggcaagg
     1681 cgccgccggt ggtgctggaa gccacgcacg agaacgtgct gcagttcgcc cccttgacgg
     1741 cgctcaagcg ccacgccctg aacgtgtcct gagcggccgg cctggccacg gcctgcgggc
     1801 gcaatccttg cctttgcatg ggccgcgctc agcgtgcccg cccggattcg ctgcttcgcc
     1861 gggcacgtgt taagctcgca ggctttttct gcggccgctc gcccccaagt taagggattg
     1921 atcccatgag cagtaccgtc cgcaacatcg ccatcatcgc gcacgtcgac cacggcaaga
     1981 ccacgctggt cgacaagctg ctgcgccagt ctggcgtgtt cgccgcgcat cagcacgtcg
     2041 aggagcgggt gatggacagc aatgcgctcg agcgcgagcg cgggatcacc atcctggcga
     2101 agaacgccgc cttcgattat cgaggcgtgc acatcaacat cgtggatacc cccggacacc
     2161 gcgatttcgg cggcgaggtg gagcgcgcgc tgtccatggt ggacggcgtg ctggtgctgg
     2221 tggatgcggt cgagggcccg atgtcgcaga cccggttcgt cacccgcaag gcgctggcgc
     2281 gggggctgcg gccgatcgtg gtggtgaaca agatcgaccg gccgggcgcg cgggcagact
     2341 gggtggtgaa ccgcaccttc gacctgttcg acaagctcgg cgccaccgag gagcagctcg
     2401 atttcccggt ggtatacgcc tccgccctgc agggtgtggc cacgctcgac ctcgctcagc
     2461 ccggccagga tctgcgaccg ctgttcgaga ccatcctgag ccgggttccg gcgcccggcg
     2521 gcgaccccga catgccgctg cagctgcaga tcagcgcgct cgactattcc agctacatcg
     2581 ggcgcatcgg catcggccgc atccggcaag ggcggctgcg cgcgggccag gaggtgctga
     2641 tcttcggcgg cgcgccgcat tcctcttcga cgaccggcgt ggaaccgcag aaagcgcgcg
     2701 tgacgcaggt gtacggcttc cagggcctgc agcgcgcgcc gctgcaggag gcgcaggccg
     2761 gcgacatcgt gctgctgcag ggcatcgagg agctgaccat cggctgcacg ctgtgcgatc
     2821 cggaggatcc gcacccgctg ccgctgctgg cggtggacga gcctacgctc accatgaact
     2881 tccaggtcaa cacttcgccc ttcgccggcc gcgagggcaa gttcgtcacc agccggcagc
     2941 tgcgcgagcg gctgcagcgg gagacgttgt cgaacgtggc gctgcgcgtg caggacaccg
     3001 ccgacgccga cgtgttccgc gtgtccgggc gcggcgagct gcatctgacc atcctgctcg
     3061 agaacatgcg ccgcgagggc tacgagctcg cggtcagcaa gccgcgcgtc gtgttcaagg
     3121 aggtcgacgg ccagcgccag gagccgatgg agctcgtcac cgccgacgtc gag
//
LOCUS       DASPGE010000190         8349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_403372, whole genome shotgun sequence.
ACCESSION   DASPGE010000190 DASPGE010000000
VERSION     DASPGE010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403372"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..886
                     /locus_tag="VD791_06030"
     CDS             233..886
                     /locus_tag="VD791_06030"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HXV07567.1"
                     /translation="MNTADEAGWPFTLPPLPFEENALAPVISSQTLHFHHGKHHRAYV
                     EKLNSLVKQRQLQDLSLVELIQCTYRVREHSEIFNNAAQVFNHNFYWQSLSPVRHQST
                     DAKPRGELARLIDDQFGNFSALKAQIVKEAIAQFGSGWVWLVAEDGKLRVMRTANAEV
                     PIIEACLPLLTLDVWEHAYYIDYQNRREEHAAAVVNRLLNWTFAAENLAHAEVAYHA"
     gene            981..1412
                     /locus_tag="VD791_06035"
     CDS             981..1412
                     /locus_tag="VD791_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07568.1"
                     /translation="MWKLTTIALATGALAMGACAHSYHDAAQAEREAALSRAERDYQQ
                     AMARCEAYSGNAEDVCEAEAKAQRAKDEASAKAEYKDTPKAEYDERIAAAEADYSVAR
                     ERCDELAGDSKEVCVKEAKATLARAKADANAAYRYGVGSNY"
     gene            1543..2367
                     /locus_tag="VD791_06040"
     CDS             1543..2367
                     /locus_tag="VD791_06040"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXV07569.1"
                     /translation="MPEATLCSLEALAATVPDGALLAVAKDASGVAMAATREVVRRGV
                     RGLHLLCVPISGMQADILIGAGCVRTLESSGVSLGEFGAAPRFVAALKERTLRMIDST
                     CPAIYAALQAGQKGIPFIPLRGLLGTDVLANRDDWKVIDNPFAPGDPIVVLQAKRPDV
                     ALFHASLADRFGNVFIGRERELMIMAHAAQRTLVTVEEITDDDLLQDQARAGAVIPSI
                     YVDKVALAPRGAWPLGLVDRYPQDEAQVARYAALARTADGFADYLNEWLGARRQAA"
     gene            2364..3215
                     /locus_tag="VD791_06045"
     CDS             2364..3215
                     /locus_tag="VD791_06045"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXV07570.1"
                     /translation="MRAAPTSPIAADTSPTACSGTELLIAVIARLLAGCRHVAVGQSS
                     PIPGSGALLARRLSSGALRVSVLGSRRNNFFTDGSVELFDLAAQGRVDAFFLGGGQID
                     GHGNINLVGTKGNLDSGGGNLDRSGGDLVSTGSNLGSPGGGHSEVRWPGSFGSAYLYF
                     LVPRVILFREEHTRRVMVPKVEFVSAPGVSRPNVYRPGGPHALLTGLGLFAFDKQRQR
                     FKLASVHPAHSVEEILDNTGFEFDRPPVVPHTVQPDAAALALIRGDIREEIAEVYPRF
                     AAALESA"
     gene            complement(3226..3477)
                     /locus_tag="VD791_06050"
     CDS             complement(3226..3477)
                     /locus_tag="VD791_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07571.1"
                     /translation="MNIFVGNLAADVTEDHLLEAFKVFGQVKSAQVKRDMFSGASKGF
                     GFVEMPGKAHSLAAIAGLNGKDFHGQPLRVNEARANGRR"
     gene            complement(3518..4891)
                     /locus_tag="VD791_06055"
     CDS             complement(3518..4891)
                     /locus_tag="VD791_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXV07572.1"
                     /translation="MRAVEEQGYAQPTPIQIKAIPAVLAGRDLLAAAQTGTGKTAGFV
                     LPILQLLMGMKGSPSGRRLPRVLILTPTRELAAQVEESVQAYGAHLPLQSTVVYGGVG
                     MQPQVQALRRGVDILVATPGRLLDHSQQKTVDLSEVKILVLDEADRMLDMGFIRDVRR
                     ILGLLPRQRQNLLFSATFSAEIRELAASFLHDPEVVEVARRNAPSELVTHVVHPVDQA
                     RKRELLAHLITSGDWRQVLVFTRTKHGANRLAQQLERDGISADAIHGNKSQSHRTRTL
                     ADFKRGELRVLVATDLAARGLDIEELPHVVNFDLPHVPEDYVHRIGRTGRAGAQGEAI
                     SLVSAEDAPLMTAIENVMKGQIERRPVAGFAPTGEQRSAQRPAPGPSRRPVRAAEPRG
                     RRPQPGRGPSRSARAEPAPRHGKRKPLPRRPSAASPGQPPRAEQKRSEKLGPMAALLA
                     GARRLIG"
     gene            complement(5117..5545)
                     /locus_tag="VD791_06060"
     CDS             complement(5117..5545)
                     /locus_tag="VD791_06060"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HXV07573.1"
                     /translation="MRCRARAAAAAVMLSCLLSCLPGQAADSIGKETGKRLLARCEPA
                     LDLLGVDSAASLSRARHADAMSCIGFVEGFLWGHGWAAWRQNRDMYYCPPEDLSGERA
                     VPTLVDYLRAHPERLDAPAHVLLFAALSQAYPCRPLPDQK"
     gene            5708..6013
                     /locus_tag="VD791_06065"
     CDS             5708..6013
                     /locus_tag="VD791_06065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HXV07574.1"
                     /translation="MKRYVVTVDFVLQPGIMAEFMPIMLDNAEKSRTLEPGCDRFDVC
                     LPAGEADRVFLYEVYRDEAAFAAHLKTEHFLQFAAATKAMIEERQVGKLWIENDDGR"
     gene            complement(6029..6550)
                     /locus_tag="VD791_06070"
     CDS             complement(6029..6550)
                     /locus_tag="VD791_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HXV07575.1"
                     /translation="MRRALAVLWAFLALAAVNLSIADKERLLASGTVVYLELAPVDPR
                     SLMQGDYMALNYAVANEARQALQASRPGERAGDLAPGDGRIVVALDERSVASFRRLDD
                     GQPLASGEVVLRYRVRAGGLKFASNAFFFEEGQGPRYEEARYGEFRVDRDGDLLLTDL
                     RGAALQRLGPTTR"
     gene            complement(6543..7613)
                     /locus_tag="VD791_06075"
     CDS             complement(6543..7613)
                     /locus_tag="VD791_06075"
                     /inference="COORDINATES: protein motif:HMM:NF025709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4401 domain-containing protein"
                     /protein_id="HXV07576.1"
                     /translation="MNGSAQRLWETLCAAGVASGHAPPGTEPHSPWYVRAMLGVAGWI
                     AAAFLFGFVGQALPWVVDSKTAALAVGTLMIAAAYALFRVEVGRDFVGQFALAMSLAG
                     QALAAIGVFGVFQPREALAWSAIAAGEAILAVLLPNFVHRVWSGYAAAMCAALALVEG
                     GAAVLAVGVVGAGAAWLWSRELAWAARSSLVRPIAYGLTLGLIQIQGDALFMQGLLGM
                     LAEDRDQAGWAQPWVGQVLTGLVLVGAVAHLVASDSRLARRDAMIALAAAVGFALLSL
                     RAPGIATGVMLVALGFAGGNRVLTGLGVAALLFYLGAYYYTLQATLLAKSAVLAATGA
                     ALLAARWALTRWLFRGREDAHA"
     gene            complement(7610..>8349)
                     /locus_tag="VD791_06080"
     CDS             complement(7610..>8349)
                     /locus_tag="VD791_06080"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HXV07577.1"
                     /translation="VQALLLAAIGVHWKLRPDRASAKAALFCAAVLMGVLLALFGQTY
                     QTGADTWELFATWAALILPWVLLARLAALWLFWIALLNVAVMLYFRVFPGVFGWISGV
                     EHMLLLLLALNTAALAAWELAARRLPWLEQRWATRLLAVGSGAAATAAALHAIFASGE
                     TSGAAAVLYPAWLVALYLVYRRMLPDLFMLAVGCLSAIVVATGGLSEHVLDWNEPGGF
                     LLIAVVVVGMAAAAAVWLRRVSVHGAA"
BASE COUNT         1439 a         2884 c         2795 g         1231 t
ORIGIN      
        1 ccttcaacgc cgggtctcgc cgggcgtgcg tatccccctg aacgcggggc cgctgcgctc
       61 gggaacgctt tctgcaccgc cttggatggg aagttcgctg cggttgcacc ggatcgccac
      121 gattcggccc gggtcaccat cattcagatg gcgaggtgat cgcaggcgga cagctggcac
      181 tgcgtcgtcc gagcgtcggt ggccggctct tgaaaccacg gagactctgc agatgaacac
      241 cgctgacgaa gccggctggc ccttcactct cccgccgttg ccatttgagg agaacgcgct
      301 ggcgccggtt atctcatcac aaacattgca ctttcatcac ggcaagcacc acagggcgta
      361 tgtcgaaaaa ctcaactcac tggtcaagca acgccagttg caggatcttt cccttgtcga
      421 gctgatccag tgcacgtacc gcgtgcggga gcactcggag atcttcaaca atgcagctca
      481 ggtcttcaac cacaacttct actggcagag cctgtcaccg gtgaggcacc agagcacaga
      541 tgcgaagccc cgcggagaac tggcacggct catcgacgac cagtttggca acttctccgc
      601 tctgaaggcg cagatcgtca aggaggcgat cgcccagttc ggcagcggtt gggtatggct
      661 ggtagcggag gacggcaaac tcagggtgat gagaacggca aacgccgagg ttcctattat
      721 cgaagcgtgc ctgccgctgc tgacgctcga tgtctgggag catgcctact atattgatta
      781 ccagaatcgg cgcgaagagc atgccgcagc cgtcgtgaac cggctgctga actggacgtt
      841 cgcggcggag aatctggcgc atgccgaggt ggcgtaccac gcatgatgct cgaatcgttc
      901 gctcgcagat gaagcgccgg cggttgagct cgcaatgtcg accgcctgct tgcgaaagta
      961 ccactcagga gaaccggtat atgtggaagc tgacaacgat cgccttggca accggcgcat
     1021 tggccatggg ggcgtgtgcc cattcgtatc acgacgccgc ccaggcggag cgcgaggcgg
     1081 cgctgagccg cgcggagcgg gactatcaac aggcaatggc tcggtgcgaa gcctattccg
     1141 gaaacgcgga ggacgtctgc gaggccgaag cgaaggccca gcgcgccaaa gacgaagcca
     1201 gtgccaaggc cgaatacaag gacactccaa aggcggaata cgatgagcgc atcgcggccg
     1261 ccgaggccga ctactcggtg gcgcgagagc ggtgcgacga gctcgccggc gacagcaagg
     1321 aggtgtgcgt gaaggaggcg aaggccacgc tggcaagggc gaaggcggac gccaacgccg
     1381 cctacaggta cggcgtcgga agcaactact gagcgcaagc aacgcggacg ctacccatcg
     1441 ccggtaagga cggcgtaacg gcagcttcgc gatggcggtc tcggctggtg cccgcatcca
     1501 tctcggagga taattgcggc aacctcagcc gggagctccg ccatgccgga agccacgctt
     1561 tgtagcctcg aagccctcgc cgccaccgtt ccggacggcg cgctgctcgc cgtcgcaaag
     1621 gatgcctccg gggtggccat ggccgcgacc cgcgaggtgg tgcgccgcgg cgtgcgcggc
     1681 ttgcatctgc tgtgcgtgcc catcagcggc atgcaggcag acatcctgat cggagcgggt
     1741 tgcgtgcgga ctctggaaag ctcgggcgtg agcctgggcg agttcggcgc cgcaccacgt
     1801 ttcgtggcgg ccttgaagga gcgtacgctg cgcatgatcg attccacctg cccggcgatc
     1861 tatgcggcgt tgcaggcggg ccagaagggc attcccttca tcccgctgcg cggtctgctc
     1921 ggcacggacg tgctcgccaa ccgcgacgat tggaaggtga tcgacaaccc gttcgcaccc
     1981 ggcgatccga tcgtcgtgct gcaagcaaag cggcccgacg tcgcactgtt tcacgccagc
     2041 ctcgccgacc gtttcggcaa cgtcttcatc gggcgcgagc gcgagctgat gatcatggcg
     2101 cacgccgcgc agcggacgct ggtgacggtg gaagagatca ccgacgacga tctgctgcag
     2161 gatcaggcgc gcgccggtgc ggtgattccc tccatctacg tggacaaggt cgcgctcgcc
     2221 ccgcgcgggg cctggccgct tggcctggtg gaccgctatc cgcaggacga ggcgcaggtg
     2281 gcgcgctacg ccgcgctcgc ccgaacggcc gatggcttcg cggactatct gaatgaatgg
     2341 ctcggcgcgc gacgccaggc cgcatgagag cagcgcccac ttcgcccatc gcggcggaca
     2401 cttcgccgac cgcctgcagc ggcaccgagc tgctgatcgc cgtcatcgcc cggctgcttg
     2461 cgggctgcag gcacgtcgcc gtgggccagt cctcgcccat ccccgggtcg ggtgctctgc
     2521 tcgcgcgccg gctgtccagc ggcgccttgc gggtcagcgt tctggggtcg cgacgcaaca
     2581 acttcttcac cgacggcagc gtggagctgt tcgatctggc cgcccaggga cgcgtcgacg
     2641 cgttcttcct gggcggcggc cagatcgacg gccacgggaa tatcaatctg gtcggcacca
     2701 aaggcaatct cgatagcggc ggaggcaatc tcgataggag cggtggcgac ctcgtcagca
     2761 ccggcagcaa cctcggcagc cccggcggcg ggcacagcga ggtgcgctgg ccaggctcgt
     2821 tcggctcggc gtacttgtac ttcctggtgc cgcgcgtgat cttgtttcgc gaggagcata
     2881 cccggcgggt gatggtgccg aaggtcgagt tcgtcagcgc tccgggcgtg agccggccga
     2941 acgtgtatcg gccgggcggg ccgcatgcgc tgctcactgg gttgggatta ttcgcgttcg
     3001 ataagcagcg gcagcgcttc aagctcgcga gcgtgcaccc ggcgcacagc gtcgaggaga
     3061 tcctcgacaa taccggcttc gagttcgacc ggccgcccgt ggtgccgcac acggtgcagc
     3121 cggacgcggc ggcactcgcg ctcatccgcg gcgacatccg cgaggagatc gccgaagtgt
     3181 atccgcggtt cgcggcggcg ctcgagtccg cctgagcccc ggcggctagc gccgcccgtt
     3241 ggcccgcgcc tcgttcacgc gcagcggctg cccgtggaag tccttgccgt tcaagccggc
     3301 gatcgcggcc agcgaatgcg ccttgcccgg catctccacg aagccgaagc ccttggatgc
     3361 accactgaac atgtcgcgct tgacctgcgc cgacttgacc tgaccaaaga ccttgaaggc
     3421 ttccagcagg tggtcctcgg tgacgtccgc tgccaagttg ccgacgaaga tgttcattcc
     3481 cgactcctgt cgaagggttt gtaggttacg tccgtgctca gccgatcaga cggcgcgcgc
     3541 ccgcgagcag cgccgccata gggccgagtt tctcgctgcg tttctgttcc gcgcggggcg
     3601 gctgcccggg gcttgcggca gaggggcgac gtggcaacgg cttgcgcttg ccatgccgtg
     3661 gggccggctc ggcacgcgcg ctgcgcgaag ggccgcgccc gggctggggc ctgcggccgc
     3721 gcggttccgc cgcgcgcacg gggcggcggg agggaccagg cgccgggcgc tgcgcgctgc
     3781 gttgctcgcc agtgggagcg aatccggcca ccggccggcg ttcgatctgc cccttcatga
     3841 cgttctcgat cgcggtcatg agcggcgcgt cctccgcgct caccagcgag atcgcttcgc
     3901 cctgcgcgcc cgcgcgcccg gtacggccga tgcggtgcac gtagtcctcg gggacatgcg
     3961 gcagatcgaa gttcaccacg tgcggcagtt cctcgatgtc caggccgcgc gcggcgaggt
     4021 ctgtggccac cagcacgcgc agctcgccgc gcttgaagtc ggccagcgtg cgggtgcgat
     4081 gcgactggct cttgttgccg tggatcgcgt cggcgctgat gccgtcgcgc tccagctgct
     4141 gcgccaggcg gttcgcaccg tgcttggtgc gcgtgaacac cagcacctgc cgccagtcac
     4201 ccgaggtgat caggtgcgcg agcagctcgc gcttgcgcgc ctggtccacc gggtgcacga
     4261 cgtgcgtcac cagctccgac ggcgcgttgc ggcgtgccac ttcgaccact tcggggtcgt
     4321 gcaggaaaga cgcggccagc tcgcggatct ccgccgagaa agtggcggag aacagcaggt
     4381 tctgccgctg ccgcggcagc aggcccagga tccggcgcac gtcgcggatg aaacccatgt
     4441 cgagcatgcg atcggcctcg tccagcacca ggatcttcac ctcgctcaag tcgacggtct
     4501 tctgttggga atgatcgagc aggcggccgg gtgtagccac caggatgtcc acgccgcgcc
     4561 tgagcgcctg cacttgcggc tgcatgccga caccgccata cacgacggtc gattgcagcg
     4621 gcaggtgcgc gccgtaggct tgcacgctct cctccacctg ggcggcgagc tcgcgggtcg
     4681 gggtgaggat caggacccgc ggcaggcgcc ggccggacgg agagcccttc atgcccatca
     4741 gcagctgcag aatgggcagc acgaatccgg cggtcttgcc ggtgccggtc tgcgcggcgg
     4801 cgagcaggtc gcgacctgcc agcacggctg ggatcgcctt gatttggatc ggagtgggct
     4861 gcgcgtagcc ctgttcttcg accgcgcgca tcagctcggc acgcaagccg agcgcggaaa
     4921 atacggaagt gatgttcttc tcctgaatcg gcctgcgacg ggcacgcgcc cgccgaaccg
     4981 gttcaggcag atgctaatga cgattgagtg ggttcgggga gttcgagaac aaccgcgaag
     5041 gggttgcggc gccgaccgga tgaggcgtcg cgagcacccg gagcccacta tacaggctaa
     5101 cgagctgtac gtatagctat ttctggtcgg gcagaggccg gcagggatac gcctgggaca
     5161 aggctgcgaa cagcagcacg tgggccggcg catccaggcg ctcgggatgc gcgcgcaggt
     5221 agtcgaccag cgtggggacg gcgcgctccc cggacaggtc ctcgggcggg cagtaataca
     5281 tgtcgcggtt ctgtcgccac gccgcccagc cgtgccccca gaggaatccc tcgacgaagc
     5341 cgatgcacga catggcgtcg gcatggcggg cgcggctcag cgacgcagcg ctgtcgacgc
     5401 caagcaggtc gagcgccggc tcgcagcgcg ccagcaagcg cttgccggtc tccttgccga
     5461 tgctgtcggc ggcctgcccc ggcaagcaag acagcagaca agacagcatg accgcggcgg
     5521 cggcagcacg cgctcgacat ctcacgcgcc gtacctcgcg cagtagaacg cctgctctac
     5581 cacgtcccag accgggcctt cgaccaggat gtcgccggca tcgccgatgc gtcggatctc
     5641 gctcattgcc ccccgagaac tgctgccagt atacttgccg gtcgcgatcc aacgtctcgc
     5701 cgccgccatg aaacgctacg tcgtcaccgt cgatttcgtc ctgcagcccg gcatcatggc
     5761 cgagttcatg ccgatcatgc tggacaacgc cgagaagtcc cgcaccctgg agccgggctg
     5821 cgaccgcttc gacgtgtgcc tgcccgcggg cgaggccgac cgcgtgttcc tgtacgaggt
     5881 ctaccgggac gaagcggcgt tcgcggcgca cctcaagacc gaacacttcc tgcagtttgc
     5941 agccgcaacc aaggcgatga tcgaggagcg ccaggtcggc aagctctgga tcgagaacga
     6001 cgacgggcgg tagcagcccg ccttgcgctc agcgggttgt cggccccagc cgctgcaacg
     6061 ccgcgccgcg caggtcggtg agcagcaggt cgccgtcgcg gtccacgcgg aattcgccgt
     6121 agcgcgcttc ctcgtagcgc ggcccctgcc cttcctcgaa gaagaacgcg ttgctggcga
     6181 acttgagccc gccggcacgc acgcgatagc gcaggaccac ctcgccactc gccagcggct
     6241 ggccgtcgtc gaggcggcgg aagctcgcca ccgaccgctc gtccagcgcg accacgatgc
     6301 ggccgtcgcc cggcgccaga tccccggcgc gttcgcccgg tcgagacgcc tgcagggcct
     6361 gccgcgcttc gttcgccacc gcgtagttga gcgccatgta atcgccttgc atcaacgagc
     6421 gtggatcgac cggcgccagc tccaggtaca cgaccgtgcc gctcgccagc aggcgctcct
     6481 tgtcggcgat cgagaggttg acagcggcga gcgcgaggaa cgcccacagc accgccagcg
     6541 ccctacgcat gcgcatcctc ccgccctcgg aacagccatc tggtcaacgc ccaccttgcc
     6601 gcgagcagcg ccgcgccggt cgcggcgagc acggcagact tcgcgagcaa cgtcgcctgc
     6661 aaggtgtagt aatacgcgcc caggtagaac agcagcgcag caacacccag ccccgtgagc
     6721 acccggttgc cgccggcgaa gccgagcgcg accagcatga cgccggtggc gatgccggga
     6781 gcgcgcagcg acagcaacgc gaaacccacc gcggcggcga gcgcgatcat cgcgtcgcgc
     6841 ctggcgagcc ggctgtcgct ggccaccaga tgcgccaccg cccccaccag caccagtccg
     6901 gtcaggacct gacccaccca gggttgcgcc cacccggcct ggtcgcgatc ctcggccagc
     6961 atccccagca ggccctgcat gaacagcgcg tcgccctgga tctggatcaa tcccagcgtg
     7021 agcccatagg cgatcggccg gaccaaagag ctgcgcgcgg cccatgcgag ctccctgctc
     7081 catagccagg cggctcccgc gccaaccacg ccgactgcga gcaccgccgc acccccttcg
     7141 acgagcgcga gcgccgcaca catggccgcc gcgtagcccg accacacgcg gtgcacgaaa
     7201 ttgggcagca gcacggcgag tatcgcctcc ccggctgcaa tcgccgacca ggcgagggct
     7261 tcgcgcggct ggaatacacc gaacacgccg atggcggcga gcgcctgccc ggcgaggctc
     7321 atcgccagcg cgaattgccc cacgaagtcg cgtccgacct cgactctgaa caacgcgtag
     7381 gccgcggcga tcatcagcgt gccgaccgcc agcgccgccg tcttgctgtc gaccacccag
     7441 ggcaaagcct gcccgacgaa gccgaacagg aacgcggccg cgatccatcc cgccacaccg
     7501 agcatcgcgc gcacgtacca gggcgagtgc ggttcggtgc ctggcggcgc gtggccggac
     7561 gcgacgccgg cggcgcacag cgtttcccac aagcgttgtg ccgatccgtt catgccgccc
     7621 catgcaccga aacgcgccgc agccagacgg ccgcggcggc ggccatgccg accacgacga
     7681 cggcgatcag cagaaagcca ccgggctcgt tccagtcgag cacgtgctcg gacaacccgc
     7741 cggtcgccac cacgatcgcc gacaggcaac ccaccgcgag catgaacagg tcgggaagca
     7801 tgcggcgata caccaggtac aaggccacga gccaggccgg gtagaggacg gcggccgcac
     7861 cgctcgtctc accggaagcg aagatcgcgt gcaacgccgc cgctgtggcc gctgctccgc
     7921 tacccactgc gagcagccgc gtcgcccagc gttgctccag ccacggcagc cgccgcgccg
     7981 ccagttccca cgccgccagc gccgcggtat tcagggcgag caggagcagc agcatgtgct
     8041 cgacgcccga gatccagccg aacacgccgg ggaacacacg gaaatacagc atgacggcta
     8101 cattgagcag tgcaatccaa aacagccaca gcgccgccag acgcgcgagc aacacccacg
     8161 gcaggatcag cgctgcccag gtggcgaaca gctcccaggt gtcggcgccg gtctggtagg
     8221 tctgtccgaa cagcgcgagc agcaccccca tcagcaccgc ggcgcaaaac agcgcggcct
     8281 tcgcggaggc ccgatcgggc cggagcttcc agtggacgcc gatcgccgcc agcaggagcg
     8341 cttgcacca
//
LOCUS       DASPGE010000191         5489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_872225, whole genome shotgun sequence.
ACCESSION   DASPGE010000191 DASPGE010000000
VERSION     DASPGE010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872225"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VD791_06085"
     CDS             complement(<1..300)
                     /locus_tag="VD791_06085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HXV07578.1"
                     /translation="MRRGRGPGFARAALLAALVVPAAPAQTPPDEFRPVRSEVESFIR
                     YMEGTHGFDARELHRLFSRLKPSEGVQRAISAPTTAKPWHEFRSLFVDQARVENGA"
     gene            complement(297..1538)
                     /gene="ccmI"
                     /locus_tag="VD791_06090"
     CDS             complement(297..1538)
                     /gene="ccmI"
                     /locus_tag="VD791_06090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HXV07579.1"
                     /translation="MIVFALLAAVLVAAALALVLRPLLRARPGAEPTPGSANRSIYRD
                     QLAELERDLSLGTLDRAQYQAARAELQRRVLEEVGAEKAPAPRSSAAATRTALAIGVA
                     LPVIAALLYWHLGQPQGIDASRQVAQDAAPPTPEQFQAMTQQLAERMAANPDDPVGWL
                     MLGRAYKALERYPEAVQALSQANRRRPGDAEILVEYAEALAQAAGGKFGREATRLLDQ
                     ALAVAPDDPKALTLAGGAALQAGDYDTAVRHWERLAAQVPPDSELGQALAAGLARARS
                     LAQGGAAPSGAPSGAEPKRGAASGPAVHGEVRLSAALKNRAAPDDTVFIFARAANGPR
                     MPLAVIRRQVKDLPADFRLDDSMAMNPELRLSAFARVVVAARVSRSGSATAQSGDLQG
                     ESAPVAPGTGRVSVVIDKVMP"
     gene            complement(1535..1984)
                     /locus_tag="VD791_06095"
     CDS             complement(1535..1984)
                     /locus_tag="VD791_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HXV07580.1"
                     /translation="MLVLAAAFAQAAEAPPAAADPAVEKRLMDLAEELRCLVCQNQSL
                     ADSNADLAVDLRNEVRSQIQAGKSDAEIRTWLTQRYGDFVLYRPPVKTTTLLLWAGPF
                     VLLVGGVTGLFVALRRRRARLAVPALTDEERARAEALLRPDSGSPPA"
     gene            complement(2002..2526)
                     /locus_tag="VD791_06100"
     CDS             complement(2002..2526)
                     /locus_tag="VD791_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HXV07581.1"
                     /translation="MTRFLIPLALFLVLVAFLAVGLDLKPREVPSPLIDRPAPAFELA
                     RLDDPAQAVRLEDLRGQVWLLNVWASWCVACLDEHPVLLEFSRSSPVPIYGLNYKDER
                     EAAIAWLGKHGNPYAASVMDAQGRVGIDYGVYGVPETFLIDREGVIRYKHIGPVTAKV
                     LSEKIVPMIGKLRS"
     gene            complement(2560..4575)
                     /locus_tag="VD791_06105"
     CDS             complement(2560..4575)
                     /locus_tag="VD791_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HXV07582.1"
                     /translation="MIPELGHFALIVALCIAVVQALVPLAGSLRGDAAWMAVARPAAQ
                     GQFVFVAIAFGCLAWSFLRNDFSVLNVASNSNSSLPAHYRFAATWGSHEGSLLLWVLM
                     LTLWMLAVTVFSRQLPRELLARVLSVMGMVSIGFLAFMLFTSNPFERLLPPAADGRDL
                     NPLLQDPGMVIHPPMLYMGYVGFSVVFAFAIAALIGGRLDAAWARWSRPWTTMAWALL
                     TVGICLGSAWAYYELGWGGWWFWDPVENASFMPWLAGTALVHSLAVTDKRGGFKIWTV
                     LLAILTFTLSLLGTFLVRSGVITSVHAFATDPARGIFILGFLAVAIGGSLALFAWRAP
                     SVGLGGKFELVSRESMLLTNNVLLLVAAGAVLLGTLYPLFLDALGLGKISVGPPYFEA
                     VFAPLMAPALFLMGVGPLARWKEARLPQLAVRLKWAFAVAVATAALLPLTLGKWTPMI
                     ALGLLLAAWIVAAGLVNLHERLTQLKATSQPLRARLRTLPRGYWGMLVAHFGVGVFLI
                     GVTMVRGYETEKDVLMRVGDSVSVAGYTFALAGLERAPGPNYESTRARIAVTRNGREV
                     ALLNPEKRFYPVQQMPMTEADMDSGFTRDLYVSLGDPVGEGAWVVRVYHKPFVDWIWA
                     GCFLMAIGGVLAVSDRRYRVSTVRDRVAVASSQGSQAADQPTVHARS"
     gene            complement(4572..5030)
                     /gene="ccmE"
                     /locus_tag="VD791_06110"
     CDS             complement(4572..5030)
                     /gene="ccmE"
                     /locus_tag="VD791_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HXV07583.1"
                     /translation="MKPRHKRLALIGTGLALLGGAAFLILNAFQSNLVFFYSPSQVAA
                     REAPQGRTFRIGGLVQNGSLQREGDGLSVRFTVTDTAQSIPVTYTGILPDLFKEGKGV
                     VAQGELGPDGVFHAREVLAKHDENYMPPEAAEAVEKAHKAMRESQKTLVQ"
     gene            complement(5027..5206)
                     /gene="ccmD"
                     /locus_tag="VD791_06115"
     CDS             complement(5027..5206)
                     /gene="ccmD"
                     /locus_tag="VD791_06115"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HXV07584.1"
                     /translation="MIWRSAAEFFAMGGYALYVWGSYGVAACLLAAELWLLARRRRTL
                     LRRLGRSLTPPERRP"
     gene            complement(5307..>5489)
                     /locus_tag="VD791_06120"
     CDS             complement(5307..>5489)
                     /locus_tag="VD791_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HXV07585.1"
                     /translation="VSLTRAPSMAATMLTGMLLMALAAWMYSIAASLMRVRAIILERE
                     ADADWARALDEGPVPE"
BASE COUNT          907 a         2003 c         1855 g          724 t
ORIGIN      
        1 tgcgccgttc tcgacccgcg cctggtccac gaacagcgac cggaactcgt gccacggctt
       61 ggccgtggtc ggtgcgctga tggcgcgctg cacgccctcg ctcggtttga gccgggagaa
      121 caggcgatgc agctcacggg cgtcgaaacc gtgcgtgccc tccatgtagc ggatgaagct
      181 ttcgacttcg gagcgcaccg ggcgaaattc gtcgggcggc gtctgcgccg gagcagccgg
      241 caccaccagc gccgccagca gcgccgcgcg cgcgaacccc ggcccccggc ctcgcctcat
      301 ggcatcacct tgtcgatcac gacggacacc cgccccgtgc cgggggccac cggcgcgctt
      361 tcgccctgca ggtcgccgct ctgcgcggtg gcgctgcccg agcgcgacac gcgcgccgcc
      421 accaccacgc gcgcgaaggc ggacagcctg agctccggat tcatcgccat cgagtcgtcc
      481 agccggaaat cggcggggag atccttgacc tgccttcgga tcaccgccag tggcatgcgc
      541 ggcccgttgg cggcgcgcgc aaagatgaat acggtatcgt caggggcggc gcgattcttt
      601 agcgccgccg acagccgcac ctcgccgtgc accgccggcc cggaggccgc tccgcgcttg
      661 ggctcggcac ccgagggcgc gccggacggc gctgcaccgc cctgagccag gctgcgcgca
      721 cgcgccagcc cggcggccag ggcctgcccc agctcggagt cgggaggcac ctgggcagcc
      781 aggcgctccc agtggcggac cgccgtatcg tagtcgccgg cctgcaatgc cgcgccgccg
      841 gcgagcgtga gcgctttcgg atcgtccggc gccaccgcca gggcttgatc cagcaggcgt
      901 gtggcctctc tcccgaactt gccgccggcc gcctgcgcca gcgcctccgc atactccacc
      961 aggatctcgg cgtcacccgg ccggcgccgg ttggcctgag acagcgcctg caccgcttcg
     1021 ggatagcgct ccagcgcctt gtaagcgcgc cccagcatca gccagcccac cggatcgtcc
     1081 gggttggccg ccatgcgctc ggcgagctgc tgcgtcatcg cctggaactg ttcgggtgtg
     1141 ggcggtgccg cgtcctgcgc gacttgccgg gaggcgtcga tgccctgcgg ctgaccgaga
     1201 tgccagtaca gcaaggccgc gatcacgggc agcgcgacgc cgatcgcgag cgcggtgcgc
     1261 gtcgccgcgg cgctgctgcg cggggcgggg gccttctccg cgccgacttc ctcgagcacg
     1321 cggcgctgca gctcggcgcg cgccgcttgg tactgggcgc ggtccagcgt tcccaggctc
     1381 aggtcgcgct ccagctcggc gagctggtcg cgatagatgc tgcggttggc gctgccgggc
     1441 gtgggctccg cccccggacg cgcgcgcagc agcgggcgca acaccagcgc cagcgccgcg
     1501 gcgacgagca ccgccgcgag cagagcgaag acgatcatgc gggcggcgag ccggagtcgg
     1561 ggcgcagcag cgcctcggcg cgggcccgct cttcgtcggt gagcgcgggc acggcaaggc
     1621 gcgcgcggcg gcggcgcagc gcgacgaaca ggcccgtcac gccgcccacc agcagcacga
     1681 acggccccgc ccacagcagc aatgtggtgg tcttcaccgg cgggcggtac agcacgaaat
     1741 cgccgtagcg ctgcgtcagc caggtgcgga tctcggcgtc gctcttgccg gcctggatct
     1801 ggctgcgcac ctcgttgcgc aggtccacgg cgaggtcggc attggaatcg gcgagcgact
     1861 ggttctggca caccaggcag cgcaactcct cggcgagatc catcagccgc ttctccaccg
     1921 ccggatcggc ggccgcggga ggcgcctccg ccgcctgcgc gaacgccgcg gccagcacca
     1981 gcatcgacac ggcgagccgc ttcacgagcg cagcttcccg atcatcggga cgatcttctc
     2041 gctcagcacc ttggccgtga ccgggccgat gtgcttgtag cggatcacgc cctcgcgatc
     2101 gatgagaaag gtctccggca cgccgtagac cccgtagtcg atgcccaccc gcccctgcgc
     2161 atccatgacc gaggcggcgt acgggttgcc atgcttgccc agccaggcga tcgcggcctc
     2221 gcgctcgtct ttgtagttca gcccgtagat cggcaccggg ctcgagcgcg agaactcgag
     2281 cagcaccggg tgctcgtcca ggcaagccac gcaccaagag gcccagacgt tgagcagcca
     2341 aacctgcccg cgcagatcct ccaggcgcac cgcctgcgcc gggtcgtcca ggcgcgccag
     2401 ctcgaacgcc ggggccggac ggtcgatgag cggtgagggc acctcgcgcg gcttgaggtc
     2461 gaggcccacg gcgaggaatg ccaccagcac cagaaagagc gccagaggga tgaggaagcg
     2521 cgtcatcggt tgtcggggtt cgtgtcggtc ttcagtgaat caagatctgg cgtgcacggt
     2581 cggctgatcc gcggcctgtg acccctgaga actggcaacc gccacccgat ccctgacagt
     2641 cgatacccga tagcgccggt cgctcaccgc cagcacgccg ccgatcgcca tcaggaagca
     2701 gccggcccag atccagtcga cgaacggctt gtggtacacc cgcaccaccc acgcgccctc
     2761 tcctaccggg tcgcccagcg acacgtacag gtcgcgggtg aagcccgaat ccatgtcggc
     2821 ctcggtcatc ggcatctgct gcaccgggta gaagcgcttc tcgggattga gcagcgccac
     2881 ttccctgccg ttgcgcgtga ccgcgatccg cgcgcgcgtg gactcgtagt tcggacccgg
     2941 tgcgcgctcc aggccggcga gcgcgaaggt gtagccggcc acgctgacgc tgtcgcccac
     3001 gcgcatcaac acgtccttct cggtctcgta gccgcgcacc atggtcacgc cgatcaggaa
     3061 cacgccgacg ccgaagtgcg cgaccagcat tccccagtag ccgcgcggca gggtgcgcaa
     3121 ccgcgcgcgc aacggctggc tcgtcgcttt gagctgggtg agccgctcgt gcagattgac
     3181 cagcccggcg gcgacgatcc aggcggcgag cagcaggccc agagcgatca tcggcgtcca
     3241 cttgcccagg gtcagcggca gcagcgcggc ggtcgccacc gcgacggcga aggcccactt
     3301 caggcgcacc gcgagctgcg gcagccgcgc ttccttccag cgcgcgagcg gacccacgcc
     3361 catcagaaac agcgccggcg ccatcagcgg cgcgaacacc gcttcgaagt aaggcggccc
     3421 caccgagatc ttgcccagcc cgagcgcgtc caggaacagc ggatagagcg tgccgagcag
     3481 caccgcgccg gcggccacca gcagcagcac gttgttggtg agcagcatcg actcgcgcga
     3541 gaccagctcg aacttcccgc ccagcccgac gctcggcgcg cgccacgcga acagcgccag
     3601 cgagccgccg atcgcgaccg ccaggaagcc gaggatgaaa atgccgcgcg ccgggtcggt
     3661 agcgaacgca tgcaccgagg tgatcacacc cgagcgcacc aggaaagtcc cgagcagcga
     3721 cagcgtgaaa gtgaggatcg ccagcagcac ggtccagatc ttgaagccgc cgcgcttgtc
     3781 ggtgacggca agcgagtgca ccagcgccgt gcccgccagc cacggcatga acgaggcgtt
     3841 ctccaccggg tcccagaacc accagccgcc ccagcccagc tcgtagtacg cccaggcgct
     3901 gccgaggcag atgccgaccg tgaggagggc ccacgccatg gtggtccacg gccgcgacca
     3961 gcgcgcccag gccgcgtcca gccggccgcc gatcagggcc gcgatcgcga aggcgaacac
     4021 caccgagaaa ccgacgtagc ccatgtacag catcggcggg tggatcacca tgccggggtc
     4081 ctgcagcagc gggttcaggt cgcgcccgtc ggccgcgggc ggcagcaggc gctcgaaggg
     4141 gttggacgtg aacagcatga acgccaggaa gccgatgctc accatgccca tcaccgacag
     4201 cacgcgcgcc agcagctcgc gcggcagctg gcggctgaac acggtgaccg ccagcatcca
     4261 cagcgtgagc atcagcaccc acagcagcag cgaaccttcg tgactgcccc aggtagcggc
     4321 gaagcggtag tgcgccggca ggctcgagtt cgagttgctg gccacgttga gcaccgagaa
     4381 gtcgttgcgc aggaacgacc aggcgaggca accaaaggcg atggcgacga acacgaactg
     4441 gccctgcgcc gcgggccgcg ccaccgccat ccaggccgca tcgccgcgca ggctgccggc
     4501 gagcggcacg agcgcctgca ccacggcgat gcacagggcg acgatcaggg cgaagtggcc
     4561 gagttccggg atcattggac cagcgtcttc tgcgattctc gcatggcttt gtgcgccttc
     4621 tccaccgcct ctgcggcctc cggcggcatg tagttctcgt cgtgcttggc cagcacttcc
     4681 cgcgcgtgga agacgccgtc gggaccgagc tcgccctgcg ccaccacgcc cttgccctcc
     4741 ttgaacaggt cgggaaggat gccggtatag gtgaccggga tgctctgcgc cgtgtcggtc
     4801 accgtgaagc gcacgctgag gccgtcccct tcgcgctgca ggctgccgtt ctgcaccagc
     4861 ccgccgatgc gaaacgtccg cccttgcggc gcctcgcggg cggccacctg cgaggggctg
     4921 taaaagaaca ccagattgct ctggaacgcg ttcaggatca ggaaggcggc cccgccgagc
     4981 agggcgaggc cggtgccgat caacgccagg cgcttgtgtc tgggcttcat ggacgccgtt
     5041 ccgggggtgt gaggctacga cccaggcggc gcagcagagt tcgccggcgg cgggcgagca
     5101 gccacagttc cgcggccagc aggcacgccg caacgccgta cgagccccat acgtacagcg
     5161 catagccgcc catggcgaag aactcggctg cgctgcgcca gatcatttca ggacaacccg
     5221 caaacggatc atttcaggac aacccgcaaa cggatcattt ccggaccacc caagaacgga
     5281 tcatttccgg accacctgcc aacgggtcat tccggaaccg gcccttcgtc cagggcgcgc
     5341 gcccagtccg catcggcctc gcgctcgagg atgatcgcgc gcacgcgcat caggctcgcc
     5401 gcgatgctgt acatccaggc ggcgagcgcc atcagcagca tgccggtcag catcgtcgcg
     5461 gccatcgacg gcgcgcgcgt caggctgac
//
LOCUS       DASPGE010000192         5950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_168997, whole genome shotgun sequence.
ACCESSION   DASPGE010000192 DASPGE010000000
VERSION     DASPGE010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168997"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VD791_06125"
     CDS             complement(<1..614)
                     /locus_tag="VD791_06125"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298861.1"
                     /note="catalyzing the hydrolysis of
                     4-imidazolone-5-propionate to N-formimidoyl-L-glutamate,
                     the third step in the histidine degradation pathway;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HXV07586.1"
                     /translation="MKGDAEGLWHNAKLLPAGDPDGAIADGAMAVRDGRIAWIGAREA
                     LPENLVGLPRHDAGGRWITPGLVDCHTHLVFAGQRADEFALRLQGASYEEIARRGGGI
                     VSTVRSTRLASEEQLFEGSAERLEALLAEGVTAVEIKSGYGLDLATERKQLRVARRLG
                     QRYPVSVYTTFLGAHAVPPEYAGRADDYIDYVAGTALPALAGEGL"
     gene            complement(622..2325)
                     /gene="hutU"
                     /locus_tag="VD791_06130"
     CDS             complement(622..2325)
                     /gene="hutU"
                     /locus_tag="VD791_06130"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HXV07587.1"
                     /translation="MNAPLSAKARRAHAARAVRAPRGSALTCKSWTIEAPYRMLQNNL
                     DPEVAERPDDLVVYGGIGRAARSWECFDAILAALRDLNEDESLLIQSGKPVGVFRTHA
                     DAPRVLIANSNLVPHWATWEHFDELDRKGLMMYGQMTAGSWIYIGSQGIVQGTYETFC
                     EAGRQHYGGDLSGRWILTAGLGGMGGAQPLAGVLAGACVLAVECQEDRIDFRLRTRYL
                     DRKATSLEEALAIIGGAVRDKQAVSVGLLGNAAQVVPELARRARTGGLRPDIVTDQTS
                     AHDPSCGYLPEGWTVAQWQDAQAHDRDGLQRAARRSIAKHVQAMLQFHDMDIPTLDYG
                     NNIRQIGFDEGVQNAFAFPGFVPACIRPLFCRGTGPFRWVALSGDPEDIYKTDRKMKE
                     LFPADRHLHRWLDLARDRIAFQGLPARICWIGLGERHRAGLAFNEMVASGELKAPIVI
                     GRDHLDAGSVASPNRETEAMRDGSDAVSDWPLLNALLNTASGATWVSFHHGGGVGIGF
                     SQHAGMVIVCDGTQAAARRIERVLWNDPASGVMRHADAGYEAAIDCARKHRLKLPSVR
                     D"
     gene            complement(2495..4054)
                     /gene="hutH"
                     /locus_tag="VD791_06135"
     CDS             complement(2495..4054)
                     /gene="hutH"
                     /locus_tag="VD791_06135"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HXV07588.1"
                     /translation="MDRLADHSGVEMVLAPGAVSLAQLRRLRDERVHVRMHEAALRDV
                     QAAQQAVSRTLERGAVAYGINTGFGKFAQTIIPPERLAELQTNLVLSHSAGTGALLGD
                     DVVRMVMALKAIGLARGYSGVRPEVIDALLRLLNARVYPCIPAKGSVGASGDLAPLAH
                     LAAALIGVGEVRVEGRQMPAREGLQRAGVEPLALAPKEGLALLNGTQVSTALGLVGLF
                     GAEDVFGAALMAGALSLEAIKGSLRPFDARIHAVRGHAGQIDVARVMHDLVQGSAILA
                     SHANCPRVQDPYSIRCQPQVMGACLDNLRHAASVLATESNAASDNPLIFADDDEILSG
                     GNFHAEPVAFVCDLLALVLAEIGALSERRIALLVDASLSGLPAFLVRDGGINSGFMVA
                     HVTAAALASDNKALSHPSTVDSLPTSANQEDHVSMATNAARRLGEMVGNSATIVGIEA
                     MAAAQGIEFHRPLTSSTTLEEMLRDIRRHVAFYERDRFLAPDIEAMKRWILSAPAHPS
                     VKAILPSTVHG"
     gene            4465..4842
                     /locus_tag="VD791_06140"
     CDS             4465..4842
                     /locus_tag="VD791_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07589.1"
                     /translation="MTPSHEEVQELSKSHARHQIVLTVLSVALVAATIMMWMLVSAVR
                     ESNEIQKELLAGSQEVDVTPKTPVKPRSVAPSSRKAGTASRRAPSASGGRQRSSPERA
                     VAQSAPRTQIASTQTATNRYGRQ"
     gene            complement(4932..5162)
                     /locus_tag="VD791_06145"
     CDS             complement(4932..5162)
                     /locus_tag="VD791_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07590.1"
                     /translation="MVAKLNLALIVALIALPAVAGQQLGRDSVYAGHKASTPTASTLA
                     TAKVLGRDSMYAFGKRASTPVTMAVTFKPGRV"
     gene            5525..>5950
                     /locus_tag="VD791_06150"
     CDS             5525..>5950
                     /locus_tag="VD791_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07591.1"
                     /translation="MRASIASLAAIALIGVGLSGHAAEGDDASESKLAQSGRALYAEG
                     VLKPAGARNKPSLAAEEETLLRSAGTSHRPARMADEDQPLKAAGASSRPGRAADDEAV
                     LKSAGTSYKPGRMADEDRPLKSTTAARPGRSTVKAAGRPG"
BASE COUNT          928 a         2073 c         2022 g          927 t
ORIGIN      
        1 agcccctcgc cggcgagcgc cggcagtgcc gtcccggcca cgtagtcgat gtagtcgtcg
       61 gcgcggccgg catactccgg cggcacagcg tgcgcaccca ggaacgtggt gtaaacgctg
      121 accggatagc gctgcccgag ccgacgcgcc acgcgcaact gcttgcgctc ggtcgccaga
      181 tccaagccgt agccggattt tatttccacg gcggtcacgc cttccgccag cagcgcttcg
      241 agccgctcag cgctgccttc gaatagctgc tcctcgcttg ccaggcgcgt gctgcgcacc
      301 gtcgagacga tgccgccgcc gcggcgcgcg atctcctcgt agctcgcccc ctgcagccgc
      361 aaggcgaact cgtccgcgcg ctgcccggcg aacaccaggt gcgtgtggca gtcgaccaat
      421 ccgggagtga tccagcggcc gccggcatcg tggcgcggca ggccgacgag gttctcgggc
      481 aatgcctcgc gggcgccgat ccaggcgatg cgcccgtcgc gcaccgccat cgccccgtcg
      541 gcgatcgcgc cgtcggggtc gcccgcgggc agcagcttcg cgttgtgcca gaggccctcg
      601 gcgtcgcctt tcatccgcgc ctcagtcgcg cacggagggc aattttaggc gatgcttccg
      661 ggcgcaatcg atcgcggcct cgtagccggc gtccgcgtgg cgcatcacgc cgctggcggg
      721 gtcgttccac aatacgcgct cgatgcgccg ggccgcggcc tgcgtgccgt cgcacacgat
      781 caccatgccg gcgtgctgcg agaagccgat ccccacgccg ccgccgtggt ggaagctcac
      841 ccaggtggcg ccgcttgcgg tgttgagcag cgcgttgagc agcggccagt ccgacaccgc
      901 gtccgagccg tcccgcatcg cctccgtctc gcggttgggg ctggccaccg accccgcatc
      961 gagatgatcc cggccgatga cgatcggcgc cttgagctcg ccgctcgcga ccatctcgtt
     1021 gaaggcgagc cccgcacgat ggcgctcgcc gagaccgatc cagcagatcc gcgccggcag
     1081 cccttggaac gcgatgcggt cgcgcgccag gtcgagccag cggtgcaggt gccggtcggc
     1141 cggaaacagc tccttcatct tgcggtccgt cttgtagata tcctccgggt cgcccgacaa
     1201 cgcgacccag cggaacggac ccgttccgcg gcagaacagc ggccggatgc aagcgggcac
     1261 gaagccgggg aaggcgaacg cgttctggac gccctcgtcg aagccgatct gacggatgtt
     1321 gttgccgtaa tcgagcgtcg ggatgtccat gtcgtggaac tgcagcatcg cctggacgtg
     1381 cttggcaatg gaccggcggg cggcgcgctg gaggccgtcg cgatcgtgcg cctgcgcgtc
     1441 ttgccactgc gcgacggtcc agccctccgg gagatagccg cagctcgggt cgtgcgccga
     1501 ggtctggtcg gtgacgatgt cggggcgcag ccccccggtg cgcgcgcgcc tcgccagctc
     1561 ggggaccacc tgcgcggcgt tgccgagcag tcccaccgag acggcttgct tgtcgcgcac
     1621 tgcgccaccg atgatcgcga gcgcttcctc cagcgaggtc gccttgcggt cgagatagcg
     1681 cgtgcgcagc cggaagtcga tgcgatcttc ctggcattcg accgccagca cgcaggcgcc
     1741 ggccagcacg ccggccagcg gctgcgcgcc gcccatgccg cccaggccgg cggtgaggat
     1801 ccagcggccg gagagatcgc cgccgtagtg ctggcggccg gcttcgcaga acgtctcgta
     1861 cgtgccctgc acgatgccct gcgagccgat gtagatccac gagcccgcgg tcatctgccc
     1921 gtacatcatc aggcccttgc gatccagctc gtcgaaatgc tcccaggtcg cccagtgcgg
     1981 caccaggttc gagttggcga tcagcacgcg cggggcgtcc gcgtgcgttc ggaacacgcc
     2041 caccggcttg ccggactgga tcaggagcga ttcgtcctcg ttcaagtcgc gcagcgccgc
     2101 gaggatggcg tcgaagcatt cccagctacg ggcggcgcgg ccgatgccgc cgtacaccac
     2161 cagatcgtcc ggtcgctccg cgacttccgg gtcgaggttg ttctgcagca tgcgataggg
     2221 cgcttcgatc gtccagctct tgcacgtgag cgcgctgccg cgcggcgcgc gcacggcgcg
     2281 tgcggcgtga gctcggcggg cctttgcgga caacggagcg ttcatgtgcg cctcctctag
     2341 tgctgaacca caccacccca ggccttcgac cgacccctcc tcttgaaagg aggggagtga
     2401 gcttcccctc ctggcaagga ggggtggcgc cgaaggcgac ggggtggtcc aagagcgatt
     2461 gcgtgccaag caaagtcagc cattgatgag aagctcaacc gtgcacggtg ctcggaagaa
     2521 tcgccttcac gctcgggtgc gccggcgccg acaggatcca gcgcttcatc gcttcgatgt
     2581 ccggggccag gaaccggtcg cgctcgtaga aggcgacgtg tcggcggatg tctcgcagca
     2641 tctcttccag cgtcgtcgag ctggtgagcg gccgatgaaa ctcgatgcct tgcgccgccg
     2701 ccatggcttc gatgccgacg atggtggcgc tgttgcccac catctcgccc aggcgccgcg
     2761 cggcgttggt tgccatcgac acgtggtctt cctggttcgc ggaggtgggc aggctgtcca
     2821 cggtggaagg atgcgacaga gccttgttgt cggacgcgag cgcggctgcg gtcacgtggg
     2881 cgaccatgaa tcccgagttg atgccgccgt cgcgcaccag gaacgccggc aatccgctca
     2941 ggctggcgtc gaccagcagc gcgatgcggc gctccgatag ggcgccgatc tcggccagca
     3001 ccagcgcgag cagatcgcac acgaaggcca ccggttcggc atggaaattc ccgccggaga
     3061 ggatctcgtc gtcgtcggcg aagatcaggg gattgtcgga ggcggcgttg ctttcggtcg
     3121 ccagcacgct cgcggcgtgg cgcagattgt ccaggcatgc ccccatcacc tgcggctggc
     3181 agcggatcga atacggatcc tgcacccgcg ggcaattcgc gtgcgaagcg agaatggcgc
     3241 tgccttgcac caggtcgtgc atcacgcgcg caacgtcgat ctggccggcg tggccacgca
     3301 cggcatggat gcgcgcgtcg aacggcctca gcgatccctt gatcgcctcg agcgacaggg
     3361 cgcctgccat cagcgccgcg ccgaacacgt cctcggcgcc gaacaacccg accaagccca
     3421 gcgccgtgga cacctgcgtg ccgttgagca gggccaggcc ttccttgggg gcgagtgcaa
     3481 gcggctctac ccccgcgcgc tgcaggccct cgcgtgcagg catttgccgt ccctcgacgc
     3541 ggacctcgcc gacaccgatc agcgcggcgg cgagatgcgc gagcggcgcc agatcgccgg
     3601 aggcgcccac cgatcccttg gccggaatgc acgggtacac gcgcgcgttc agcaggcgca
     3661 gcaaggcgtc gatcacctcg ggacgaaccc cggaatagcc ccgtgcgagg ccgatcgcct
     3721 tcagggccat caccatgcga acgacgtcat cgcccagcag cgcaccggtg cctgcgctgt
     3781 gcgacaggac caggttggtc tgcagctcgg ccagccgctc cggggggatg atcgtctggg
     3841 cgaacttgcc gaaaccggtg ttgatgccgt aggcgaccgc cccgcgctcc agggtgcggc
     3901 tgacggcctg ctgagccgcc tgcacgtcac gcagcgccgc ctcgtgcatt cgcacatgca
     3961 cgcgctcgtc gcgcaggcgg cgcaactggg ccaggctcac ggcgccgggc gccagaacca
     4021 tttccacgcc gctgtggtcg gccaatcgat ccatgtgtgt ggtccctgtt cccgggggca
     4081 cccggttgcc gcaccttccg ggccccgcgc ggtctggggg aggcgtgccg cagcgctttc
     4141 gctcccgcag gtgcgatggc gtctttatcg gccgacagcg cccccctcta tagctcgccg
     4201 tgcgtggcga gtgggaaggc gcctctctgc tatcttcgga cttccgactg cgcgaagcgc
     4261 gtgcttcccc gcggcgcgac gcgtttgcgc gtcccgcgca ctctgtgcac catcgtcaaa
     4321 tcctcgtcgc cgactcccga caatggaccg tcgcggtgct caagccgagc gccctcgcga
     4381 cgttacctgc actaggcact gcgaggcgag atcgtcggat ctgcaaggtg ccgtcccagc
     4441 agctgcgtca cacggaggcg gacgatgacc ccatcccacg aagaggttca ggaactttcc
     4501 aaatcgcacg cgcgtcacca gatcgtgctc acggtcctgt ccgtggcctt ggtggcggcg
     4561 acgatcatga tgtggatgct ggtgagcgcg gtgcgcgagt ccaacgagat ccagaaggag
     4621 ttgctggcgg gttcgcagga ggtggacgtt acgccgaaga cacccgtgaa gccgcggtcg
     4681 gtcgcacctt catcacgcaa ggcaggcacc gcatcgcgtc gtgcgccgag cgcctccggc
     4741 gggcggcaac ggagcagccc ggagcgggcg gtcgcccaga gcgcgccccg cacccagatc
     4801 gccagcacgc aaacggcgac gaatcgttac ggccgccagt aggctgcggc tcgggggctc
     4861 gctccggaat aaaaaggcca cccggcgtac cgggtggccc aaagtggaga tggctctcca
     4921 ggggaaacgc tttacacgcg gccgggcttg aacgtcaccg ccatagtgac gggggtcgag
     4981 gccctcttgc cgaacgcgta catgctgtcg cggcccagca ccttggcggt ggccagcgtg
     5041 ctggcggtcg gagtgctcgc cttgtgtccc gcgtagacgc tgtcgcggcc cagttgctgg
     5101 ccggctacgg cgggaagcgc gatcaaagcg acgatcagcg cgaggttgag cttggcaacc
     5161 atgagaacct ccttacgagt gacttgattg agcggtttgc tcacgttgtc caggttacct
     5221 gcccatcgcc gctcgggccc gtaacaattc ccgcccgggc gcgtgaaaat tcacgccccg
     5281 caacccggcc tggcgggtct gacccggctc ggaatcgagc gacgtcttat acggcgcgtg
     5341 agtgcacctg gatcaccccc gaaccgggca gtcctgaagg gttcctaaag tgatagcgcc
     5401 ttccgaggaa ggggtgttgc ccgccgcggc cgcctttgcc atcattcgtt cggggatctg
     5461 cgccccgcgt cgggcgccgg atccggcgtc agacaccgat cgggcgtcag acaggagaac
     5521 agcaatgcgt gcatcgatcg cctcgcttgc cgccatcgca ctcatcgggg ttggcctctc
     5581 cggccatgcc gccgaaggag acgacgcctc tgaatccaag ctcgcgcagt cgggccgggc
     5641 cttgtatgcg gagggcgtcc tcaagccagc aggcgcgagg aacaagccca gtctggccgc
     5701 tgaagaggaa acgctcctca gatccgccgg cacgtcgcac aggcctgcgc gcatggccga
     5761 tgaggaccag ccgctgaagg cggcaggcgc ctcgagcagg ccgggtcgcg ccgcagacga
     5821 cgaggccgtc ctcaaatccg ccgggacttc ctacaagccg ggtcgcatgg ccgacgagga
     5881 caggccgctc aagtccacga ccgccgccag gccgggccgc tcaaccgtga aggcggccgg
     5941 gcgccccggc
//
LOCUS       DASPGE010000193        16742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_520958, whole genome shotgun sequence.
ACCESSION   DASPGE010000193 DASPGE010000000
VERSION     DASPGE010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16742
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520958"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..674)
                     /gene="mscL"
                     /locus_tag="VD791_06155"
     CDS             complement(249..674)
                     /gene="mscL"
                     /locus_tag="VD791_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HXV07592.1"
                     /translation="MSFMSEFKEFAVKGNVIDLAVGVIIGAAFGKIVNSIVNDLIMPL
                     VGRVVGNADFSNLFVALAEVPPGVPDTLDALRKAGVPVFAYGSFLTVLLDFIILAFVI
                     FLMIKQFNRLKRQAPPPAPAAPPEEVVLLRDIRDSLRRA"
     gene            complement(863..1351)
                     /locus_tag="VD791_06160"
     CDS             complement(863..1351)
                     /locus_tag="VD791_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HXV07593.1"
                     /translation="MRASLLTCLVIAFSLAGCAGAQVVPDAQVVPGDRPGPSVANDSA
                     AQAADAALAQVGRPYRYGGDTPAGFDCSGLVRYSYLQVGVPVSRETRTLRGQARLIRA
                     ADLRRGDLVFFDQEGKKYSHVGIYVGDGHFVHAPSSGGRVRTDRLDSHYWKRHFVEAR
                     RI"
     gene            complement(1461..3128)
                     /locus_tag="VD791_06165"
     CDS             complement(1461..3128)
                     /locus_tag="VD791_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006414609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXV07594.1"
                     /translation="MKPADYKIWPKQFPKSLSVPRATLWHNLEVAAARYPDKPITIFY
                     DSQLGYGRFRREAEALAGWLQQRCGVQKGERVLLDMQNSPQFMLGYYACLRADAVVVP
                     VSPMNVTDELEHYVADSGARVALIGQEVYAQFRPLLDKELEHVLVATYSDYLSASTPL
                     EVPAVLGAPRETFAEPGVAGWREALALQCVPGAPAATTDDLSAILYTSGTTGKPKGCM
                     HTHGTVMCTAVGGALWEGMAPHSVALATAPMFHVTGMQHSLNAPIFMGASIALLPRWD
                     PAAAAYMIERYRVTHWANVPTMVVDLLAHPDTERRDLSSLQNVFGGGASMPEAVAQRL
                     YDLCGIEYMEAYGMTETISQTHINPPGDLRKQCLGIPTFDTESMIVDPDTLQPLPPGE
                     TGEILSCGPQLFKGYWNNPQATAAALVQIGGRQWLRTGDLGRVDQDGFFYIADRLKRM
                     INAAGYKVWPAELEATLYKHPDIKEVAIVSAPDPRRGETVKAYVVLKDGAQGRVSGED
                     LIAWCRQHMAAYKVPRQVQFLDALPRSGTGKIQWRTLQEKEWAGGAS"
     gene            complement(3143..4831)
                     /locus_tag="VD791_06170"
     CDS             complement(3143..4831)
                     /locus_tag="VD791_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXV07595.1"
                     /translation="MQPSATEHSQTPRHYRFWPRGLPHSLEFPATGLYFNLEVSAARY
                     PDKPLMLFYDAVLTYAQAKAQADALAAFLQQRCGVRRGDRVLLFMQNSPQFVIGYYAC
                     LRADAMVVPINPMNLTEELRHYVADSGATVALCGQELYPQLAPLLGAGLEHALVAAYS
                     DYLSPRTDLDVPDSVRAPRQSIVAPGVTLWRDALDAGLQPDPHLAGPDDRCVMPYTSG
                     TTGRPKGCIHTHATVMSTVVIAAQWAGGHQDSVILGTLPMFHVTGMQGSMNIPIYVGA
                     SVVMMTRWDRHVAARLIERYRVDSWTNIATMAIDFLANPDLGRYDLGSLQRIGGGGAA
                     MPEAVAQRLKDRTGLDYVEGYGLSETIGPTHINPSERPKKQCLGIPICNTDARVIDPD
                     TLRELGPGEVGEIVCSGPQVFKGYWNSPQASAACFVQLGGMRFFRTGDLGRYDEDGYF
                     FIADRLKRMINASGYKVWPAEVESIMYGHADVQEACVIGARDPLRGETVKALVVLKAD
                     SRGKISPEDLIGWCRGHMAAYKIPRVIEFVDSLPKSATGKVQWRALQEREMQRK"
     gene            4998..5828
                     /locus_tag="VD791_06175"
     CDS             4998..5828
                     /locus_tag="VD791_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV07596.1"
                     /translation="MQEAAAPAAPPLSASDTPLRVTDIPLLRVTDVTVRFGGIVALDR
                     VSFDVEAGLICGLIGPNGAGKTTLFNCLSRLYPFQEGSILFDGRPLLEVPRHGIASLG
                     IGRTFQNLALFRSLTVRENVMLGAHARTRGGFLACALRLPGARREERELIAHAGELMH
                     LVDLERYALRRVADLPFGIQKRVELARGLAARPRLLLLDEPAAGLNHAEIEGLMQVIR
                     RIRDELEVTVLLVEHHMNLVMRTSDKVVALDFGKKIADGTPDQVRNDPDVIRAYLGKA
                     "
     gene            5828..6541
                     /locus_tag="VD791_06180"
     CDS             5828..6541
                     /locus_tag="VD791_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV07597.1"
                     /translation="MAALLEVADLQAGYGPLTVLHGIEFQVEDGGITTLLGANGAGKT
                     TTLRAVCGMLRPRGRIALAGEAIAGRATEDIVRRGVAHVPEGRGTFLGLSTEENLRVG
                     AYTRRDRPGVAEDMERCYHYFPRLKERRHQQAGTLSGGEQQMLAIARALMLRPRLLLL
                     DEPSFGLAPLVVQEIFRIMELIRRERRVGILLVEQNASLALEVADRAYLLETGRVVLG
                     GAAAQVKQDEAVRKSYLGY"
     gene            6544..7962
                     /locus_tag="VD791_06185"
     CDS             6544..7962
                     /locus_tag="VD791_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXV07598.1"
                     /translation="MRDYDRYDAIGLAALVAAREVSPQELLDAAIARTEQVNPRINAV
                     VAKHYDEARAAIRAGLPQGPFRGVPFLLKDLHALLTGTITSNGCRFYADNRADHDTEL
                     VARYKRGGLTIFGKTNTPEFGLTVTTEPLLFGPTHNPWNLEHMAGGSSGGAAAAVAAG
                     IVPAAHASDGGGSIRIPASCCGLVGLKPTRARNPHGPDRGEGWSGMSTEHVVSRSVRD
                     SAALLDLTHGPDLGAPYFATPPERPFLEALGAERTPLRVGLVTRTPSGDAVDPECERA
                     TRNAAKLLDGLGCRVEEAALSSIEEGFGPAFRIVVAGNIRAAIELQAARVGRQPQAAD
                     FEKITWLFFESGARASAADYARAVITLHRTGRQIARLFQNCDLLLTPTLPKPPQKIGV
                     FSMMTDDPEVYGREVAYFTSFTALVNIAGNPAVSLPLHWTPDGLPVGVQLIGRYGDEA
                     MLLRVAAQLEQAQPWFHHRPTL"
     gene            7965..8834
                     /locus_tag="VD791_06190"
     CDS             7965..8834
                     /locus_tag="VD791_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV07599.1"
                     /translation="MEALLHQVLSGLATGGIYASLALALVMIYRSTNHINFAQGEMAM
                     FSTYLAWTLIDAGVSWWLAFLITVAASFAAGMAIERLLIRPVEHAPLLTVVIVFIGLL
                     VIFNSVAGWIYSYTIKSFPSPFPAEPVFGGLMSAHELGAIGVTLVVLLLLYVFFQFTP
                     LGLAMRAVAQNPASSRLAGIRVGWMLALGWGLAAAIGAVAGMMVAPVIYLDPNMMSGV
                     LLYAFAAALMGGIDSPGGAVAGGFIVGVVENVMGAFVIGTELKLTVALLVIIGVLLVR
                     PSGLFGKVFVTRV"
     gene            8837..9820
                     /locus_tag="VD791_06195"
     CDS             8837..9820
                     /locus_tag="VD791_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014899814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV07600.1"
                     /translation="MARQARWWQWLGLLAALALLCALPFVLSNYRTFQFTLALVYAIV
                     LLGLNILTGYNGQISLGHGAFYAIGAYTAAILMDKFGVPYWATVPLGGAVCLVFGFLF
                     GLPALRLHGHYLALATFALAVAMPQILKYKGIEEWTGGVQGIVIIKPDPPAGVPLNQD
                     QWLYLLTLAVAVAMFALAWNLIHSRVGRAWMALRDHPIAAEAMGVNSALYKASAFGIS
                     ALYTGVAGALGAIVVQFVAPDSFTGVLSINFLIGVVIGGLASISGAIWGALFIQFVPN
                     IADQISKAAPWAIYGIFMIAFAYAMPTGVAGFLRLVWSRASRRAAGVGVRA"
     gene            9913..11121
                     /locus_tag="VD791_06200"
     CDS             9913..11121
                     /locus_tag="VD791_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV07601.1"
                     /translation="MAYAYRALAAALAAGLVFAAAAHAQKRYGPGASDTEIKFGQTMP
                     YSGPASAYGTIGKAEAAYFRMINDQGGVNGRKLNLISLDDGYSPPRTVEQIRKLVEDE
                     QVLFAFQTLGTPSNTAIHKYMNGRKVPQLFVATGATKWGDPQHYPWTMGWQPTYQHES
                     QIYAKYLLKNRPNAKIAVLFQNDDYGKDYLKGFKDGLGDQVSKLIVAEASYEVTDPTV
                     DSQIVALKASGADVLFNITTPKFAAQAIRKVHDIGWRPLHFLNVVSTSVAAVLQPAGL
                     DKSVGLVSVAYFKDPTDPAWKDDKAVQDYLAFMKKYYPDGNPADAFNVYGLAVAQTLV
                     QTLRQCGDDLTRENLMRQAANLKDVEVPMLLPGIRINTSPTDFYPIEQVQLIRFDGKT
                     WVRFGEIVSR"
     gene            11199..12422
                     /locus_tag="VD791_06205"
     CDS             11199..12422
                     /locus_tag="VD791_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV07602.1"
                     /translation="MTDGIVPMRFSRFAAVALACALAAPAFAQKKYGPGVTDTEIKLG
                     QTMPYSGPASAYGAIGKAELAYFRMVNERGGINGRRVNLISLDDAYAPPRAVEQVRRL
                     VEEDDVLALFQTLGTPTNTAFHKYVNVKKVPHLFLATGATKWGDPKHYPWTIGFQPAY
                     QVEARIYARYILSNKPDAKIAVLYQNDDYGKDYVKGLRDGLGDKARSMIVAEVSYEVT
                     DATVDSQIVTLKSSGADTFFNVTTPKFAAQAIRKAHDIGWKPLHIINNVASSVGAVLI
                     PAGLDKSTGLITALYFKDPNDPQWAKDAGMQRWRDFMARYMPGADISDFNYVYGYGAA
                     QLMTQVLAACGDDLTRENLMKQAISLRDMTLDVTLPGVKFSTGPNDYYLIEQLQMARF
                     DGKRWVLFGEVIGQE"
     gene            12443..13198
                     /locus_tag="VD791_06210"
     CDS             12443..13198
                     /locus_tag="VD791_06210"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV07603.1"
                     /translation="MKIDLEGKVAVITGASRGIGRATALALGGAGAKLALVGRDREKL
                     AESLQLAREAGADAHTYVCDVRDEAQVKRLAADVSERFGAVHILVNNAGTAIRKNVID
                     FSLEEWRALTDTNITGVFLMCKYFVPHMRGHGYGRIINLTSIMAHISSGGRGVYSATK
                     HAVAGLTKALALELVDDGINCVAISPGFVATDLTAPLRADPAKNAAIMAQTPMKRWGR
                     PEEIAALALYLCSDAAAFMTGNDVLIDGGWCAQ"
     gene            13553..15475
                     /locus_tag="VD791_06215"
     CDS             13553..15475
                     /locus_tag="VD791_06215"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HXV07604.1"
                     /translation="MNSAIQVWLDQQCTQIDGAAGGVVMLLPRGGKAPLAAAASWPAG
                     AAANEELEAAAKTAYEREAPQTQPRLNGTQRPVPLGPVISHPIQVKGRTIGALAVCLG
                     PEATLPPQSALESVERGVGSFETFMRGQLSAGKKAKSGSASRGFAPSNPAPQLPAAPA
                     RGAASGSPSAPRKPSEDLERTAEMPAPNSVRILHLLATLEAHEGFRAAATAFATELTE
                     MFDCDRVAVGLVGRRHVKVEALSHSSEFKSNQGLLRDIGAAMEEAIWQGSTLVFPQPD
                     GAQPRVDRAHATLASRHGAIAIYTIPLVKAGKPVGAITLERSRTDPLPRSEVTLGENI
                     GALLGPLLEMKRTADRPWPLKIWQALREAIAPLTGPGHRLTKIVTGCAVALLAAAALI
                     SGEYRVSAPARLEGAMQRVLVAPGEGYLKQAHVRPGDRVKEGQLLAEMADEDLKLEQR
                     KAQSAVAQLETSLGVAMVSGNRAEVGMLQARLDEARAQLMLVETQLERVRLTAPFDGV
                     VITGDLTQSLGAPVKKGEALLTLAPEHDFRVMLEIDERDIGDVKLGQAGTLALAALPG
                     KPLAFDIHRITPVATAGDGRNFFEVEARVRDKSAELRPGLQGVGKVEAGSRSLLWIWT
                     HRVVGWIRLTLWSWIG"
     gene            15555..>16742
                     /locus_tag="VD791_06220"
     CDS             15555..>16742
                     /locus_tag="VD791_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07605.1"
                     /translation="MEGLFSSRWYRVAGVHPRLRSHVHVSRHVYRGQVWYLLQDQSSG
                     RHHRVDEIAFQFIGRMDGQRSTDEIWHSLLNQLGERTPTQDETIEILCQLSDNDLLQC
                     EITPNVAELFRRRFEKDRKRRLAMLNPLAFKVPLFDPDKLLERLTPLARVLFRPATLV
                     LWVLAMVLALLSAGSNWDSIAAYAKVHMLTPRFLLLLWLCYPFIKAIHELGHGLAVKA
                     WGGEVRETGISLLLLVPVPFVDASAASAFPEKHRRAVVGAAGIMVELLLAALALFVWS
                     SVEDGLTRQIAFVVMVVGGVSTVLFNGNPLLRFDGYFVLSDVLDVPNLGPRANAYIGY
                     LTQRYVLKVENAVSPVTARGEPPLLFGYALGAFGYRWFVAGLIVFWCGSYSFWLGMLA
                     AALV"
BASE COUNT         2603 a         5678 c         5822 g         2639 t
ORIGIN      
        1 aacctgctgc cgttcctggc ggcgcccgcc ggcggcatcg cgcacggcgc gcacatcggc
       61 gggttcatcg ccggcgcgat ggtggcgttg atcctgcggc gtaagcgacg gtgacgcgcc
      121 gagtttgccg ccgggagcgg aagcagtcaa cgtagcccgg atggagccgc agcaggcggc
      181 gcaatccggg gacgagaagg ggtactcctc acccccggat tccgcttcgc gcatccgggc
      241 tacgctcgct acgctcgcct cagcgaatcg cggatgtcgc gcaacagcac cacctcctcc
      301 ggcggagcag cgggcgcggg gggcggagcc tggcgcttga gccggttgaa ctgcttgatc
      361 atcaggaaga tcacgaacgc caggatgatg aagtcgagca gcacggtgag gaaactgccg
      421 taggcgaaca ccggcacgcc ggccttcctg agcgcgtcca gggtgtcggg aacgcccggc
      481 gggacctcgg caagcgcgac gaacaggttc gagaaatcgg cgttgccgac gacgcgtccc
      541 accagcggca tgatcaagtc attgacgatc gaattcacga tcttgccgaa agccgcaccg
      601 atgatgacgc cgaccgccaa gtcgatgacg ttgcccttga cggcgaactc cttgaactct
      661 gacatgaagc tcatgacgcg ggtctccttg tggttggatt tggacaaagt gcgggctgcg
      721 cgcaaattct aacccgggcc ctcgatggtc gatgcaagca aagctggcgt agcctggagg
      781 gagcgaagcc gaatccgggg aacagaaccg ccttgtcatc ccggattgcg ctgcgctccg
      841 ggctacgcgg gctgcggcgc cgctagatcc gccgcgcctc gacgaaatgc cgcttccagt
      901 agtggctgtc caggcggtcg gtgcgcaccc ggccgccgga cgacggcgcg tgcacgaagt
      961 gcccgtcgcc gacgtagatg cccacgtgcg agtacttctt gccttcctgg tcgaagaaca
     1021 ccaggtcgcc gcgccgcaga tccgcggcgc ggatcaggcg cgcctgcccg cgcagcgtgc
     1081 gggtttcgcg cgacacgggc acgccgactt gcaggtagct gtaccgcacc agcccgctgc
     1141 agtcgaaccc ggccggcgta tcgccgccgt accgataggg gcgaccgacc tgggcgagcg
     1201 cggcgtcggc ggcttgcgcc gcactgtcat tcgccacgga ggggcctggc ctgtcgccgg
     1261 ggacgacctg ggcgtccggt acgacctgag cgccggcaca gccggccagc gagaaggcga
     1321 tgacaaggca ggtcagcaga ctggctctca tgggtgcgcg cgacggtgga tccggcagcc
     1381 ttggcgtgca agatctgcgc cggcagacgc gcgatcgcct ggcccgaatt tgtcatgtcg
     1441 gcgcgcgact acggctgagg ctatgacgcg ccgccggccc actccttctc ctgcagcgtg
     1501 cgccactgga tcttgccggt gcccgagcgc ggcagcgcgt cgaggaattg cacctgccgc
     1561 ggcaccttgt aggcggccat gtgctggcgg caccaggcga tcaggtcctc gccgctcacc
     1621 ctgccctgcg cgccgtcctt cagcaccacg taggccttca ccgtctcgcc gcgccgcggg
     1681 tcgggcgcgg acacgatggc cacttccttg atgtccgggt gcttgtacag cgtggcctcc
     1741 agctccgccg gccacacctt gtagccggcg gcgttgatca tgcgcttcaa gcggtccgcg
     1801 atgtagaaga acccgtcctg atccacgcgg ccgaggtcgc cggtgcgcag ccactgcctg
     1861 ccgccgatct gcaccagcgc ggccgcggtg gcctgcgggt tgttccagta acccttgaac
     1921 agctgcggcc cgcacgacag gatctcgccg gtttcgccgg gcggcagcgg ctgcagcgtg
     1981 tcgggatcca cgatcatgga ctcggtgtcg aaggtcggga tgcccaggca ttgcttgcgc
     2041 agatcgcccg gcggattgat gtgggtctgc gagatggtct cggtcatccc gtaggcttcc
     2101 atgtactcga tgccgcacag gtcgtagagc ctttgcgcaa ccgcttcggg catggacgcc
     2161 cccccgccga acacgttctg cagcgacgac agatcgcgcc gctcggtgtc cgggtgcgcg
     2221 agcaagtcca ccaccatggt cggcacgttg gcccagtgcg tgacgcggta gcgctcgatc
     2281 atgtacgcgg cggcggccgg atcccagcgc ggcagcagcg cgatgctcgc gcccatgaag
     2341 atcggtgcgt tcaggctgtg ctgcatgccg gtcacgtgga acatgggcgc ggtggcgagc
     2401 gccaccgaat gcggcgccat gccttcccac agcgcgccgc ccaccgccgt gcacatcacc
     2461 gtgccgtgcg tgtgcatgca gcccttgggc ttgccggtcg tgcccgaggt atagaggatc
     2521 gcgctcaggt cgtcggtcgt cgcggccggc gccccgggca cgcactgcag ggccagcgcc
     2581 tcgcgccagc cggccacccc gggctctgcg aaagtctcgc gcggcgcgcc gagcacggcc
     2641 ggcacctcga gcggcgtgga agcgctgagg tagtccgagt aggtcgccac cagcacatgc
     2701 tccagctctt tgtccagcaa cgggcggaac tgggcgtaga cctcctgccc gatcaacgcg
     2761 acgcgagcgc cgctgtcggc gacgtagtgc tccagctcgt cggtcacgtt catcgggctg
     2821 accggcacca ccacggcgtc ggcgcgaaga caggcgtaat agccgagcat gaactgcggg
     2881 ctgttctgca tgtcgagcag tacgcgctcg cccttttgca cgccgcagcg ctgctgcagc
     2941 cagccggcga gcgcctcggc ctcgcgccgg aaccggccgt agccgagctg cgagtcgtag
     3001 aagatcgtga tcggcttgtc cgggtagcgc gccgccgcca cctcgaggtt gtgccacaga
     3061 gtggcgcgcg ggacgctgag gctcttggga aactgcttcg gccagatctt gtagtccgcg
     3121 ggtttcatct tctcgacatt ggctacttgc gctgcatttc gcgctcctgc agcgcgcgcc
     3181 actgcacctt gccggtggcc gacttgggca ggctgtccac gaactcgatt acgcgcggga
     3241 tcttgtacgc ggccatgtgc ccgcggcacc agccgatcag gtcctcgggc gagatcttgc
     3301 cccggctgtc ggccttgagc accaccagcg ccttgaccgt ctcgccgcgc agcgggtcgc
     3361 gggcaccgat cacgcacgcc tcctgcacgt cggcatggcc gtacatgatc gattccacct
     3421 cggccggcca caccttgtag ccggaggcgt tgatcatgcg cttcaagcga tcggcgatga
     3481 agaagtagcc gtcctcgtcg tagcgaccca gatcgccggt acggaagaag cgcatgccgc
     3541 cgagctggac gaagcaggcg gcgctcgcct gcgggctgtt ccaatagccc ttgaacactt
     3601 gcggaccgct gcacacgatc tcgcccacct ccccggggcc cagctcgcgc aacgtgtcgg
     3661 ggtcgatcac gcgcgcatcg gtattgcaga tggggatgcc caggcactgc ttcttcgggc
     3721 gctcgctcgg gttgatgtgc gtgggtccga tggtctccga cagcccgtag ccttccacgt
     3781 agtccagccc ggtgcggtcc ttcaggcgct gcgcgaccgc ctcgggcatg gccgcgccgc
     3841 cgccgccgat gcgctgcagg ctgccgaggt cgtagcgccc gaggtccgga ttggccagga
     3901 agtcgattgc catggtcgcg atgttggtcc agctgtccac gcggtagcgc tcgatcagcc
     3961 gcgcggccac gtgacggtcc cagcgcgtca tcatgaccac gctcgccccg acgtagatcg
     4021 gtatgttcat gctgccctgc atgcccgtga catggaacat cggcagcgtg cccaggatca
     4081 cgctgtcctg gtggccgccg gcccattgtg cagcgatgac caccgtgctc atcaccgtgg
     4141 cgtgggtgtg gatgcagcct ttcggtcgcc cggtggtgcc agaggtgtag ggcatgacgc
     4201 accggtcgtc gggacccgcg aggtgcggat cgggctgtaa gccggcgtcg agcgcgtcgc
     4261 gccacagcgt cacgccggga gccacgatcg actgccgcgg ggcgcgtacc gaatccggaa
     4321 cgtccaggtc ggtgcgcggc gagaggtaat cgctgtaggc ggcgaccaac gcgtgctcca
     4381 ggccggcacc gagcagcggt gcgagctgcg gatagagctc ctgcccgcac agcgccacgg
     4441 tggcaccgct gtcggcgacg taatggcgca gctcctcggt caggttcatc gggttgatcg
     4501 gcaccaccat ggcgtcggcg cgcaggcagg cgtagtagcc gatcacgaac tgcgggctgt
     4561 tctgcatgaa cagcagcacg cggtcgccgc gccgcacccc acagcgctgc tgcaggaagg
     4621 cggcgagcgc atcggcctgc gccttggcct gcgcgtaggt gagcaccgca tcgtagaaca
     4681 gcatcagcgg cttgtccgga tagcgggcgg ctgacacctc gaggttgaag tagaggccgg
     4741 tggccgggaa ctcgagcgag tgcggcagcc cgcgcggcca gaaccgatag tggcggggcg
     4801 tctgactgtg ctcggtggcg gacggctgca tgcgctgtgg cttgtgtggt cgagtgcgcc
     4861 gctattctaa atggcgggcg gcgccccgcg tggctttgcc ggcccgggcc atttgtgtca
     4921 gtatcgcccc caaaccaagg ccgggccgcg ctgcgagccc ggccgcgcac gacgagagga
     4981 gaccccgagt ggggaggatg caggaggcgg ccgcgcccgc cgcgccgccg ctgagcgcgt
     5041 cggatacccc gctgcgggtg accgacatcc cgttgttgcg ggtcaccgat gttacggtgc
     5101 gcttcggcgg catcgtcgcg ctcgaccgcg tctccttcga tgtcgaggcc ggtctgatct
     5161 gcggcctgat cgggccgaac ggcgccggca agaccaccct gttcaactgc ttgtcgcggc
     5221 tgtacccgtt ccaggagggc agcatcctgt tcgacggccg gccgctgctg gaagtgccgc
     5281 gtcacggcat cgccagcctc ggcatcggcc gcaccttcca gaatctcgcg ctgttccgca
     5341 gcttgacggt gcgcgagaac gtcatgctcg gcgcgcacgc ccggacccgc ggcggcttcc
     5401 tggcctgcgc gctgcgcctg ccgggcgcgc gccgcgaaga gcgcgagctg atcgcgcacg
     5461 cgggcgagct gatgcatctg gtggacctgg aacgctatgc gttgcggcgg gtggcggatc
     5521 tgccgttcgg catccagaag cgcgtggagc tggcgcgggg gcttgccgcg cgtccgcgcc
     5581 tgctgctgct ggacgagccg gcggccggac tgaatcacgc ggagatcgag gggctgatgc
     5641 aggtcatccg ccgcatccgc gacgagctcg aggtcaccgt gctgctggtg gagcatcaca
     5701 tgaacctggt gatgcgcacc tcggacaagg tggtggcgct ggacttcggc aagaaaatcg
     5761 ccgacggcac tcccgaccag gtgcgcaacg acccggacgt gatccgggcc tatctgggca
     5821 aggcgtgatg gctgcgctgc tcgaggtcgc cgatctgcag gccggctacg ggccgctgac
     5881 ggtcctgcac ggcatcgagt tccaggtcga ggacgggggc atcaccacct tgctgggcgc
     5941 aaacggcgcg ggcaagacca ccaccttgcg cgccgtgtgc ggcatgctgc ggccgcgcgg
     6001 caggatcgcg ttggcgggcg aggcgatcgc cggccgcgcc accgaggaca tcgtgcgccg
     6061 gggcgtcgcg cacgtgcccg aagggcgcgg cactttcctc ggtctgagca ccgaggagaa
     6121 cctgcgcgtg ggcgcctaca cgcgccgcga caggcccggc gtggccgagg acatggagcg
     6181 ctgctatcac tacttccccc gactcaagga gcggcggcat cagcaggccg gcacgctgtc
     6241 gggcggcgag cagcagatgc tcgccatcgc ccgcgcgctg atgctgcgcc cgcgcctgct
     6301 gctgctcgac gagccgagct tcggactggc gccgctggtg gtgcaggaga tcttccgcat
     6361 catggagctg atccgacgcg aacgccgcgt cggcatcctg ctggtggagc agaacgccag
     6421 cctggcgctg gaggtggccg accgcgccta cctgctggaa accggccgcg tggtgctcgg
     6481 tggggcggcg gcacaggtca agcaggacga ggcggtgagg aaatcctacc tgggatacta
     6541 gcgatgcgag attacgaccg ctacgacgcc atcggcctgg ccgcgctggt cgccgcccgc
     6601 gaggtgtcgc cgcaggaatt gctggacgcc gcgatcgcgc gcaccgagca ggtcaacccg
     6661 cgcatcaacg cggtggtggc caagcattac gacgaggcgc gcgcggcgat ccgcgccgga
     6721 ctgccgcagg ggccgtttcg cggcgtgccg ttcctgctga aggacctgca cgcgctgctc
     6781 accggtacga tcaccagcaa tggctgccgc ttctacgccg acaaccgcgc cgatcacgac
     6841 accgagctgg tggcccgcta caagaggggc gggctgacga tattcggcaa gaccaacact
     6901 ccggaattcg ggctcacggt gaccaccgag ccgctgctgt tcgggcccac gcacaacccg
     6961 tggaacctcg agcatatggc cggcgggtcg agcggcggcg cggccgccgc cgtggcggcg
     7021 ggcatcgtac cggctgcgca cgcgtccgac ggcggcgggt cgatccgcat tccggcctcg
     7081 tgttgcggcc tggtcgggct caagcccacg cgcgcgcgca atccgcacgg tcccgaccgc
     7141 ggcgagggct ggtcgggcat gtccaccgag cacgtcgtgt cccgcagcgt gcgcgacagc
     7201 gcggcactgc tggacctgac ccacggcccg gacctgggtg cgccttactt cgcgacgccg
     7261 cccgagcgcc ccttcctgga ggcgctcggc gccgagcgca cgcctctgcg cgtgggcctg
     7321 gtgacgcgca cgccgtcggg cgacgcggtc gacccggagt gcgagcgcgc cacacgcaac
     7381 gccgccaagc tgctcgacgg gctcggctgc cgcgtcgagg aagccgcgct gtcgtcgatc
     7441 gaagagggct tcggcccggc gttccgcatc gtcgtcgcgg gcaacatccg cgccgccatc
     7501 gagctgcagg ccgccagggt gggccgccag ccccaggcgg cggacttcga gaagatcacc
     7561 tggctgttct tcgagtccgg cgccagggcg agcgccgccg actacgcgcg cgcggtcatc
     7621 acgctgcatc gcaccgggcg gcagatcgcg cgcctgttcc agaactgcga tctgctgctg
     7681 acgccgaccc tgcccaagcc gccgcagaag atcggggtgt tcagcatgat gaccgacgat
     7741 cccgaggtct acgggcgcga agtggcctat ttcacctcgt tcactgcgct ggtcaacatc
     7801 gccggcaatc ccgccgtgtc cctgccgctg cactggacgc cggatgggct gccggtgggg
     7861 gtgcagctca tcggccgcta cggcgacgag gccatgctgc tgcgcgtggc ggcgcagctc
     7921 gagcaggcgc agccctggtt ccaccaccgc ccgacgctct agccatggaa gcgctgctgc
     7981 atcaggtgct ctcgggcctc gcaacgggcg gcatctacgc cagcctggcg ctggcgctgg
     8041 tgatgatcta ccggtccacc aaccatatca acttcgccca gggcgagatg gcgatgttct
     8101 cgacctatct ggcgtggacg ctgatcgacg cgggggtgag ctggtggctc gctttcctga
     8161 tcacggtggc cgcgtccttc gccgccggca tggcgatcga gcggctgctg atccgcccgg
     8221 tggagcacgc gccgctgctg acggtggtga tcgtgttcat cggcctgctg gtgatcttca
     8281 acagcgtggc ggggtggatc tacagctaca ccatcaagag cttccccagc ccgttcccgg
     8341 ccgagccggt gttcggcggt ttgatgtcgg cgcacgagct gggcgccatc ggcgtgacgc
     8401 tggtggtgct gctgctgctg tacgtgttct tccagttcac cccgctcggc ctggcgatgc
     8461 gggcggtggc gcagaacccc gcgtccagcc gcctggccgg gatccgggtg ggatggatgc
     8521 tggcgctggg ctggggactc gcggccgcca tcggcgcggt cgccggcatg atggtcgcgc
     8581 cggtgatcta cctcgatccg aacatgatgt cgggcgtgct gctgtacgct ttcgccgcgg
     8641 cgttgatggg cgggatcgac agtcccggcg gcgcggtggc cggcggcttc atcgtcggcg
     8701 tggtggagaa cgtgatgggc gccttcgtga tcggcaccga gctgaagctc accgtggcgc
     8761 tgctggtgat catcggggtg ctgctggtca ggccgagcgg cctgttcggc aaggtgttcg
     8821 tgacgcgggt gtgaacatgg cccggcaggc gcgctggtgg cagtggctgg gcctgctggc
     8881 cgcgctggcg ctgctgtgcg cgctgccttt cgtgctgagc aactaccgca ccttccagtt
     8941 caccctggcg ctggtgtacg ccatcgtgct gctggggctg aatatcctca ccggctacaa
     9001 cggccagatc tcgctcgggc acggcgcgtt ctacgccatc ggcgcctaca ccgccgccat
     9061 cctgatggac aagttcggcg tgccgtactg ggccaccgtt cccctgggcg gcgccgtgtg
     9121 cctggtgttc ggcttcctgt tcgggctgcc ggcgctgcgg ctgcacggcc actatctcgc
     9181 gctcgcgacc ttcgcgctgg cggtggcgat gccgcagatc ctcaagtaca agggcatcga
     9241 ggagtggacc ggcggggtgc agggcatcgt gatcatcaag cccgacccgc ctgcgggcgt
     9301 gccgctcaac caggaccagt ggctctacct gctcacgctg gcggtcgcgg tggcgatgtt
     9361 cgcgctcgcc tggaacctga tccacagccg cgtgggccgg gcctggatgg cgctgcgcga
     9421 ccatccgatc gccgccgaag ccatgggcgt gaacagcgcc ctgtacaagg ccagcgcctt
     9481 cggcatcagc gcgctgtaca ccggggtcgc cggcgcgctg ggcgcgatcg tggtgcagtt
     9541 cgtggcgccc gacagcttca ccggcgtgct gtccatcaac ttcctgatcg gcgtggtaat
     9601 cggcggcctg gcctcgattt cgggcgcgat ctggggcgcg ctgttcatcc agttcgtgcc
     9661 caacatcgcc gaccagatct ccaaggccgc gccctgggcg atctacggca tcttcatgat
     9721 cgccttcgcc tacgccatgc ccaccggcgt ggccggattc ctgcgcctgg tttggtcgcg
     9781 cgcgtcgcgg cgcgcggctg gtgtcggcgt gcgagcgtag cccggacgcc gcgcagcggg
     9841 atccggggac ggtaacgccg tgcgcgcgaa gccggcatat aatcgcgcga cacgacttgg
     9901 ggaggaggcg tcatggcata cgcataccgt gcactggccg ccgcgctcgc ggcgggcctc
     9961 gtgttcgctg cggcggccca cgcgcagaag cgctacggtc cgggcgcgag cgacaccgag
    10021 atcaagttcg ggcagaccat gccctacagc gggccggcct cggcctacgg caccatcggc
    10081 aaggccgaag cggcctactt ccgcatgatc aacgaccagg gcggcgtcaa cggccgcaag
    10141 ctcaacctga tctcgctcga cgacggctac agcccgccgc gcaccgtgga gcagatccgc
    10201 aagctggtcg aggacgagca ggtgctgttc gccttccaga cgctgggcac gccttccaac
    10261 acggcgatcc acaagtacat gaacgggcgc aaggtgccgc agctgttcgt ggccaccggg
    10321 gcgaccaagt ggggcgaccc gcagcactac ccctggacca tgggctggca gcccacctat
    10381 cagcacgaga gccagatcta cgcgaagtat ctgctgaaga accggccgaa cgcgaagatc
    10441 gcggtgctgt tccagaacga cgactacggc aaggattatc tgaaaggctt caaggacggc
    10501 ctgggcgacc aggtgtcgaa gctgatcgtg gccgaggcga gctacgaggt caccgatccc
    10561 accgtggatt cgcagatcgt ggcgctgaag gcctccggcg ccgacgtgct gttcaacatc
    10621 accacgccga agttcgcggc gcaggcgatc cgcaaggtgc acgacatcgg ctggcggccg
    10681 ctgcacttcc tgaacgtggt gtccacctcg gtggcggcgg tgctgcagcc cgccgggctg
    10741 gacaagtccg tcgggctggt gtcggtggcg tacttcaaag atcccaccga cccggcctgg
    10801 aaggacgaca aggcggtgca ggactatctc gcgttcatga agaagtacta ccccgacggc
    10861 aatcccgccg acgctttcaa cgtctacggc ctcgcggtgg cgcagacgct ggtgcagacg
    10921 ctcaggcagt gcggcgacga tctcacgcgc gagaacctga tgcggcaggc ggccaacctc
    10981 aaggacgtcg aggtgccgat gctgctgccg ggcatccgca tcaacaccag tccgaccgac
    11041 ttctacccga tcgagcaggt gcagctgatc cgcttcgacg gcaagacctg ggtgcgcttc
    11101 ggcgagatcg tgtcgcgctg aaggccggcc gccggtttgt cgggcccgtt tgttgtgacg
    11161 gcgcgcgcga tgcataatgt gcgcttcccc aaccattctt gaccgacggc atcgttccca
    11221 tgcgcttctc ccgcttcgcc gccgtggcgc tcgcctgcgc gctcgccgca cccgccttcg
    11281 cccagaagaa atacggtccc ggcgtcaccg acaccgagat caagctcggc cagaccatgc
    11341 cctacagcgg gcccgcctcg gcctacggcg ccatcggcaa agcggagctc gcctacttca
    11401 ggatggtcaa cgagcgcggc ggcatcaacg gccgcagggt caacctgatc agcctggacg
    11461 acgcctacgc gcccccgcgc gcggtggagc aggtgcgcag gctggtcgag gaagacgatg
    11521 tgctggcgct gttccagacg ctgggcacgc cgaccaacac ggccttccac aagtacgtca
    11581 acgtgaagaa ggtgccgcac ctgttcctcg ccaccggcgc caccaagtgg ggcgatccga
    11641 agcactaccc gtggaccatc ggcttccagc cggcctacca ggtcgaggcc aggatctacg
    11701 cccgctacat cctgagcaac aagcccgacg cgaagatcgc ggtgctctac cagaacgacg
    11761 actacggcaa ggattacgtg aaaggcctca gggacggcct gggcgacaag gcgcgcagca
    11821 tgatcgtcgc cgaggtgagc tacgaggtca ccgatgccac cgtggactcg cagatcgtca
    11881 cgttgaagtc ctcgggggcc gacaccttct tcaacgtcac cacgcccaag ttcgcggcgc
    11941 aggccatccg caaggcccac gacatcggct ggaagccgct gcacatcatc aacaacgtgg
    12001 cctcgtcggt cggtgcggtg ctgatcccgg ccgggctgga caagtccacc ggcctgatca
    12061 ccgcgttgta cttcaaggac cccaacgacc cgcagtgggc caaggacgcg gggatgcagc
    12121 gctggcgcga cttcatggcc aggtacatgc ccggtgccga catcagcgat ttcaactacg
    12181 tgtacggcta cggcgcggcg cagctcatga cccaggtgct cgctgcctgc ggcgacgacc
    12241 tcacgcgcga gaacctgatg aagcaggcga ttagcctgcg cgacatgacg ctggacgtga
    12301 cgctgcccgg ggtcaagttc agcaccggcc ccaacgacta ttacctgatc gagcagctgc
    12361 agatggcgcg cttcgacggc aagcgctggg tgctgttcgg cgaagtgatc gggcaggagt
    12421 gaggcggaag gagcgcacag cgatgaagat cgacctcgaa ggcaaggtgg cggtgatcac
    12481 cggcgcgagc cgcggcatcg gccgcgcgac ggcgctcgcg ctgggcggcg ccggtgcgaa
    12541 gctggcactg gtggggcgcg accgggagaa actcgccgag tcgctgcagc tcgcgcgcga
    12601 ggccggggcg gatgcgcaca cctacgtgtg cgacgtgcgc gacgaggcgc aggtgaagag
    12661 actcgcagcc gacgtgtccg agcgcttcgg cgcggtgcac atcctggtga acaacgccgg
    12721 caccgcgatc cgcaagaacg tcatcgactt ctcgctggag gagtggcgcg cgctcaccga
    12781 caccaacatc accggcgtgt tcctgatgtg caagtacttc gtgccgcaca tgcgcgggca
    12841 cggctacggg cgcatcatca atctcacctc catcatggcg cacatctcca gcggcggacg
    12901 cggcgtgtac tccgccacca agcacgcggt ggccgggctc accaaggcgc tggcactgga
    12961 gctggtggac gacggcatca actgcgtggc gatcagcccc gggttcgtcg ccaccgacct
    13021 cactgcgccg ctgcgcgcgg atccggcaaa gaacgccgcg atcatggcgc agacgccgat
    13081 gaagcgctgg ggccggccgg aggagatcgc cgccctcgcg ctgtacctat gctcggatgc
    13141 cgccgcgttc atgaccggca acgacgtgct gatcgacgga gggtggtgcg cgcagtagcg
    13201 cgccggctgc gcgcgagcat gcacaggccg ggcgctgccc ggcctgtttc tttgtgcgcc
    13261 gctggcgggc agttccagcg ctccccggca cgcgggtcgg tacgacccag ggcgctcgca
    13321 cgcggcttgc gacatcgcct gcagtagtgc gcgggattcg tttcacattc gaaccgccac
    13381 tctttcagca acgtcaattg catcggcaca aagcttgcag aaggcttgcg ccgagagctt
    13441 gcccccctcg gcgcaaattg agaggtgtgc agccgggagg tgtcgcgatt ctggtcccgg
    13501 gcaggacccc tgtcgcgagc gcgaccgctt tcaccgatgc atcgacaata ctatgaactc
    13561 ggccatccaa gtctggctcg atcagcagtg cacgcagatc gacggcgccg ccggcggcgt
    13621 ggtcatgctg ctgccccgcg gcggcaaggc gccgctcgcg gccgcggcat cctggcccgc
    13681 gggcgcggct gcgaacgagg agctggaggc cgccgccaaa acggcgtatg agcgtgaggc
    13741 cccgcagacg caaccgcgtc tgaacggcac gcaacggccg gtcccgctcg gaccggtgat
    13801 ctcgcatccg atccaggtga agggacgcac catcggtgcg ctcgccgtgt gcctgggccc
    13861 ggaagcgacc ctgccgccgc agagcgcgct ggagagcgtc gagcgcggag tgggatcctt
    13921 cgagaccttc atgcgcggtc agctctccgc cggcaagaag gcgaagagcg gctcggcgag
    13981 ccgcggattc gcacccagca accccgcccc gcagctgccc gccgccccgg cacgcggcgc
    14041 ggcttcgggc tcgccctctg caccgcgcaa gccgagcgag gatctcgagc gcacggccga
    14101 gatgcccgca ccgaactctg tgcgcatcct gcacctgctg gccacgctcg aagcgcacga
    14161 gggcttccgg gcggcggcca ccgccttcgc caccgagctg acggagatgt tcgactgcga
    14221 tcgcgtggcc gtcggtctgg tcggacgccg gcacgtgaag gtcgaggcgc tgtcccacag
    14281 cagcgagttc aagagcaacc agggcctgct gcgcgacatc ggcgcggcga tggaagaggc
    14341 gatctggcag ggctcgacgc tggtcttccc gcagcctgac ggcgcacagc cgcgcgtgga
    14401 ccgcgcgcac gcaacgctcg cgtcgcggca cggtgcgatc gccatctaca ccattccgct
    14461 ggtcaaggcg ggcaagccgg tgggcgccat cactctggag cgcagccgca ccgacccgct
    14521 gccgcgctcc gaagtgaccc tgggcgagaa catcggcgcg ctgctcggcc cgctgctgga
    14581 gatgaagcgc accgcggacc gcccttggcc gctgaagatc tggcaggcgc tgcgcgaagc
    14641 gatcgcgccg ctcaccggtc ccggccaccg gctgaccaag atcgtgaccg ggtgcgcggt
    14701 ggcgctgctc gcggccgccg cgctgatctc gggcgaatac cgggtgagcg cgccggcgcg
    14761 cctggaaggc gccatgcagc gcgtgctggt ggcgccgggc gaaggttatc tgaagcaggc
    14821 gcacgtgcgt cccggcgacc gtgtcaagga aggccagctg ctggccgaga tggcggacga
    14881 ggacctcaag ctcgagcagc gcaaggcgca aagcgcggtg gcgcagctcg agacctcgct
    14941 cggcgtggcg atggtgagcg gcaaccgcgc cgaggtcggc atgctgcagg cacggctcga
    15001 cgaggcgcgc gcgcagctga tgctggtcga gacgcagctc gaacgcgtgc ggctgaccgc
    15061 gcccttcgac ggcgtggtga tcaccggcga cctgacgcaa tcgctgggcg cgccggtgaa
    15121 gaagggcgag gcgctgctca cgctggcgcc ggagcacgac ttccgcgtca tgctggagat
    15181 cgacgagcgc gacatcggcg acgtcaagct cggccaggcc ggcaccctgg cgctggcggc
    15241 cctgccgggc aagccgctcg ccttcgacat ccaccgcatc acgccggtgg ccaccgccgg
    15301 cgacgggcgc aacttcttcg aagtcgaggc gcgcgtgcgc gacaagagcg cggagttgag
    15361 gcccgggttg cagggcgtgg gcaaggtcga ggccggctcg cgctcgctgc tgtggatctg
    15421 gacgcaccgc gtggtcggct ggatacgcct caccttgtgg tcgtggatcg gctagccatg
    15481 agatgaacag gctgcggccg cgagtcaccc caacaacgaa tcacgagtca cgaatcacga
    15541 ctcacggacc ctagatggaa ggcctgttca gctcccgctg gtaccgcgtg gccggcgtgc
    15601 atccgcggct gcgcagccac gtgcacgtct cgcgccacgt ctaccgcgga caggtgtggt
    15661 acctgctgca ggaccagagc tcgggccgcc accaccgcgt ggacgagatc gcctttcagt
    15721 tcatcgggcg catggacggc cagcgctcga ccgacgagat ctggcactcg ctgctcaacc
    15781 agctcggcga gcgcacgccc acccaggacg agaccatcga gatcctgtgc cagctgagcg
    15841 acaacgatct gcttcagtgc gagatcacgc cgaacgtcgc ggagcttttc cggcgccgct
    15901 tcgagaagga ccgcaagcgc cgcctggcga tgctcaaccc actggcgttc aaggtgccgc
    15961 tgttcgaccc ggacaagctg ctcgagcgcc tgaccccgct ggcgcgcgtg ctgttccggc
    16021 cggcgacgct ggtgctgtgg gtgctggcga tggtgctggc gctgctgtcg gccggctcca
    16081 actgggattc gatcgccgcg tacgccaagg tgcacatgct cacgccgcgc ttcctgctgc
    16141 tgctgtggct gtgctatccg ttcatcaagg cgatccacga gctgggccac ggcctggcgg
    16201 tcaaggcctg gggcggcgaa gtgcgcgaga ccggaatcag cctgctgctg ctggtgccgg
    16261 tgccgttcgt ggacgcttcg gcggcctcgg cgttcccgga gaagcaccgg cgcgcggtgg
    16321 tgggcgcggc cggcatcatg gtcgagctgt tgctggcggc gctggcgctg ttcgtgtgga
    16381 gcagcgtcga ggacggtctc acgcgccaga tcgccttcgt ggtgatggtg gtgggcgggg
    16441 tgtccacggt gctgttcaac ggcaacccgc tgctgcgctt cgacggctat ttcgtgctgt
    16501 cggatgtgct cgacgtgccc aacctcggcc cgcgcgccaa cgcctacatc ggctacctca
    16561 cgcagcgcta cgtgctcaaa gtggagaacg ccgtctcgcc ggtcaccgcg cgcggcgaac
    16621 ctccgctgct gttcggttac gcgctgggcg ccttcggcta ccgctggttc gtggccggcc
    16681 tgatcgtgtt ctggtgcggc agctattcgt tctggctggg catgctggcc gcggcgctgg
    16741 tg
//
LOCUS       DASPGE010000194         3268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_404183, whole genome shotgun sequence.
ACCESSION   DASPGE010000194 DASPGE010000000
VERSION     DASPGE010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404183"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1289
                     /locus_tag="VD791_06225"
     CDS             <1..1289
                     /locus_tag="VD791_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXV07606.1"
                     /translation="ALDRERVLEDARKADQKRTAGEASGALHGLPLPVKDSINIADYR
                     TTGGTSALRAFQPRRDAAVMKPLRAAGAIVLGKTNVHELSYGWTSNNEAFGPVRNPYD
                     RSRIPGGSTGGTAAAVAARMAPAGLAEDTMGSIRIPAALSGICGLRPTTGRWPQRGVI
                     PISPLFDTVGPHARTVADLALLDAVVTGNTRLAPMSSLRGVRLAVSPAYFLAGLHPET
                     ERVTQAALARLEEAGAQIVEAEVPELADMVNGMAFQIGLWDTVPSIRAYLKEQSLDMT
                     FEDILEQMGPGVRWVFENFSLPGAQFFPTREAYENARKFLRPKIQETFRRYFRENDVQ
                     ALVFPSALCPATRIGEDAETEWGGEKKQIFMVYSRNIAPGNCASLPGLVLPGGLTADG
                     LPVGIEFDAPIGKDRELLGLGFALERALGPIPAPNV"
     gene            1348..2172
                     /gene="pssA"
                     /locus_tag="VD791_06230"
     CDS             1348..2172
                     /gene="pssA"
                     /locus_tag="VD791_06230"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HXV07607.1"
                     /translation="MNYPEHPQLKPKWLAAGGRIRRTGIYWLPNAITTLALFAGFYAI
                     VQAMNGRFELAAMAVFVAMVLDGMDGRVARLTRTQSPFGAEYDSLSDMVSFGAAPALV
                     IYEWALKGMGRMGWVAAFVYCACAALRLARFNTNIGVIDRRFFQGLPSPAAAGLIAGF
                     VWTMEAFDLEPHRVYEMRWLAWGLAVFAGLTMVSNVRFYSGKDINLRKAVPFSAVVLI
                     VLLIVILSQVVDNLPELLFSIFAIYAASGYVHWAVTRLQRKTPPRPPPPSMPPDGL"
     gene            2563..>3268
                     /locus_tag="VD791_06235"
     CDS             2563..>3268
                     /locus_tag="VD791_06235"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HXV07608.1"
                     /translation="MKQRLIIFDTTLRDGEQSPGASMTREEKMRIARQLERLRVDVIE
                     AGFPAASNGDFEAVKAIADTIKDSIVCGLARANDSDIRRAGEALRDATAGRIHTFIAT
                     SPIHMERKLRMTPDQVIEQAVRSVKLARSYREDVEFSPEDAGRSEPDFLCRILEQAIK
                     AGATTINIPDTVGYTMPEQFGNLIRNLRERIPNSDKVTWSVHCHNDLGLAVANSLSAV
                     MNGARQVECTVNGLGER"
BASE COUNT          532 a         1144 c         1086 g          506 t
ORIGIN      
        1 tcgctctcga ccgcgagcgc gtactcgagg acgcgcgcaa ggccgaccag aagcggaccg
       61 cgggggaagc atccggtgcg ctgcacggct tgcctctccc ggtgaaggac agcatcaata
      121 tcgccgacta tcgcaccacc ggcggcacca gcgcgctgcg cgcgttccag cccagacgag
      181 acgcggctgt gatgaagccg ctgcgcgccg cgggcgcgat cgtgctgggc aagaccaacg
      241 tccacgagct gtcctacggc tggaccagca acaacgaggc gttcggcccg gtgcgcaatc
      301 cctacgaccg cagccgcatc cccggcggca gtaccggcgg caccgcggcg gcggtcgcgg
      361 cacgcatggc gcccgcgggc ctggccgagg acaccatggg ctcgatccgc attcccgccg
      421 cgctgtcggg catctgcggg ctgcgaccca ccaccggccg ctggccgcag cgcggcgtca
      481 ttccgatctc gccgttgttc gataccgtcg gcccgcacgc gcgcaccgtg gccgacctgg
      541 cgctgctcga cgcggtggtg acgggcaata cgcgccttgc cccgatgtcg tccctgcgcg
      601 gcgtgcgcct tgcggtgtcg cctgcgtact tcctcgccgg cctgcacccc gagaccgagc
      661 gcgtgacgca ggcggcgctc gcgcgcctcg aggaggcggg ggcgcagatt gtggaggcgg
      721 aggtgcccga gctcgccgac atggtcaacg gcatggcgtt ccagatcggg ctgtgggata
      781 cggtgccttc catccgcgcg tatctcaagg agcagagctt ggacatgacc ttcgaggaca
      841 tcctcgagca gatggggccc ggcgtgcgct gggtattcga gaacttcagc cttcccggcg
      901 cgcagttctt ccccacgcgc gaagcctacg agaacgcgcg caagttcctg cggccgaaga
      961 tccaggagac cttccggcgc tacttccgcg agaacgacgt gcaagcgctg gtgtttcctt
     1021 cggccctgtg ccccgcgacg cgcatcggcg aagacgccga gaccgagtgg ggcggcgaga
     1081 agaagcagat tttcatggtg tactcgcgca acatcgcccc cggcaactgc gccagcctgc
     1141 ccggcctggt gctgcccggc gggctgaccg ccgacgggct gccggtgggc atcgagttcg
     1201 acgcgccgat cggcaaggac cgcgagctgc tcggcctcgg gttcgccctg gagcgggcgc
     1261 tggggccgat tcccgctccg aacgtctgag gctcaagtcg cgcggctccg taccgaaact
     1321 tcggcctgtg gccaccacaa tccacgcatg aactatcccg agcatcccca actcaagccg
     1381 aaatggctgg ccgcgggcgg acgcatccgc cgcaccggca tctactggct gccgaacgcc
     1441 atcaccacgc tggcgctgtt cgccggcttc tatgccatcg tgcaggcgat gaacggcagg
     1501 ttcgagctgg ccgcgatggc ggtgttcgtc gccatggtgc tcgacggcat ggacgggcgc
     1561 gtggcccggc tcacgcgcac gcagagcccg ttcggcgcgg aatacgacag cttgtccgac
     1621 atggtgtcgt tcggtgcggc gccggcgctg gtgatctacg agtgggcgct caaaggcatg
     1681 ggccgcatgg gctgggtggc ggccttcgtg tactgcgcct gcgcggcgtt gcggctggcg
     1741 cggttcaaca ccaacatcgg cgtgatcgac aggcgcttct tccagggatt gccgagcccg
     1801 gccgccgccg gcctcatcgc cgggttcgtg tggaccatgg aggctttcga ccttgagccg
     1861 catcgcgtct acgagatgcg gtggctggcg tgggggctgg cggtgttcgc cggtctgacc
     1921 atggtcagca acgtgcgctt ctacagcggc aaggacatca acctgcgcaa agcggtgccc
     1981 ttcagcgcgg tggtgctgat cgtgctgctg atcgtgatcc tgtcccaggt ggtcgacaac
     2041 ctgccggagc tcctgttcag catcttcgcg atctatgccg cctccggcta cgttcactgg
     2101 gcggtgaccc gtctccagcg caaaacaccg ccacggccgc cgccgccgtc gatgccgccc
     2161 gacgggctgt agcccggatg gagcgcagcg gaatccgggg atgagatggc tcctttctcc
     2221 cggattccgc cgccagttca atcgacaacg catttggcgc ggctgcatcc gggctccgcg
     2281 gccgtgacgt gccgcgaagc gacgcccgac ccccttgcgc cggcacccga aacgccttat
     2341 attcgaccca tcatgcatag cgcaggccgc ctcactctcg ccctcctcgc cagcctcctg
     2401 ctggcgggcc tgctgctgcg cgttcgcgcg cacacacact aatcccattt cctggcagta
     2461 tccggcggtc ccgcaagggg ccggtgatcc attggtacgc gccctcgatc tcaccccctc
     2521 gccccaggcc gcgaggacag cgggccaaac aggagaacga cgatgaaaca gcgcttgatc
     2581 atattcgaca ccacgctgcg ggacggcgag cagagccccg gtgcatcgat gacgcgcgag
     2641 gagaagatgc gcatcgcacg gcagctcgag cgcctgcgcg tggacgtgat agaagccggc
     2701 ttcccggccg caagcaacgg cgatttcgag gcggtcaagg cgatcgccga caccatcaag
     2761 gacagcatcg tgtgcggcct ggcgcgcgcc aacgacagcg acatccgccg cgccggagaa
     2821 gcgctgcgcg atgccaccgc cgggcgtatc cacaccttca tcgccacctc gccgatccac
     2881 atggagcgga agctgcgcat gacgcccgac caggtcatcg agcaggcggt gcgatcggtg
     2941 aagctggcgc gcagctaccg cgaggacgtg gaattctcgc ccgaggacgc cgggcgctcc
     3001 gagcccgact tcctgtgccg catcctggag caggcgatca aggccggcgc caccaccatc
     3061 aacatccccg acaccgtcgg ctacaccatg ccggaacagt tcggcaacct catccgcaac
     3121 ctgcgcgagc gcattcccaa ctcggacaaa gtgacgtggt cggtgcactg ccacaacgac
     3181 ttggggctgg ccgtggccaa ctcgctcagc gcggtgatga acggcgcacg ccaggtggag
     3241 tgcacggtga acggcctggg cgagcgcg
//
LOCUS       DASPGE010000195        11902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_52878, whole genome shotgun sequence.
ACCESSION   DASPGE010000195 DASPGE010000000
VERSION     DASPGE010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11902
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52878"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VD791_06240"
     CDS             complement(<1..615)
                     /locus_tag="VD791_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXV07609.1"
                     /translation="MNNSAVTLIGLLEAGGADAVALSAPGGVPLTYGALRALVARTVA
                     ALNARGIGRNDRVAIVLDNGPEMAAAFICVAAGATAAPLNPTYRAEEFEFYLRDLGAK
                     VLMVERGRPSPALEMAQKLGVPVVRLAPQPELGAGVFTLEFPDSMQPKPARSPGPAAE
                     GDIALVLHTSGTTSRPKIVPLTQRNLCASARNVCRTLALTAADRG"
     gene            722..1594
                     /locus_tag="VD791_06245"
     CDS             722..1594
                     /locus_tag="VD791_06245"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HXV07610.1"
                     /translation="MRIEPIPAGGTLGATIEGIDLACASGTDVDKLIDALGRYGVLRF
                     PRQSLSATDLKNFSARLGELEINVAGTFQEPGHPQVMILSNIVENGQPIGLADAGQGW
                     HADMSYSRVVAFANVLYAVKVPQHGGRPLGATQFCDMCAAYDGLPNETKRQLEGKSAL
                     HDFNKFWETMRRRPGSTRPPLTEAQRQTKPPVSHPIVLTHPISGRKALYANPGYTTRV
                     DGLPQAESDRLLEFLFAHQTRDEYRYVFHWQVGDVLVWDNLRVIHQAVADYGPEEHRL
                     MKRCQVMATRFSPG"
     gene            1599..2558
                     /locus_tag="VD791_06250"
     CDS             1599..2558
                     /locus_tag="VD791_06250"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXV07611.1"
                     /translation="MAKLASALILVGLTIAAGAAWAQTYPSQPIRIIVPFGPGSGSDI
                     VGRRLGIYLQERWKQPVVIDNRPGVQGMIGTEALRSAPADGYTLAMSTNSTHAAALHL
                     IKNLPYDPIADFEHIALIGTTGSVALVRRDSPFKSIPELAAYARAHPGKVFFGHADTI
                     SQIPGELLRAAAKLPVEGVAYKTSANVIVDLIGGQIQFAFFNYMTGAAQAASGRLVPI
                     AITESSRSSRWPAVPTLSETYPGLEVTAFVGVTAPHGVPPQIVAKLHRAIQEAQDDPG
                     FREPLEGGGLTFVPLPLGGYRSFILRETQRWREHVKTAGLVAR"
     gene            complement(2555..3562)
                     /locus_tag="VD791_06255"
     CDS             complement(2555..3562)
                     /locus_tag="VD791_06255"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HXV07612.1"
                     /translation="MRIAVVGAGAIGGYLGARLSAAGEEVVFIARGANLAAIRTDGMR
                     LIGEDGAEVRSVGARAVEKPAEAGAQDTVLLTVKAHQVAAIAPELRHLCHADTAIVTM
                     QNGIPWWYFHRHGGQYEGTPVHSADPDGSICRHIDPARIIGSVVYPAANLIAPGVVQV
                     VEGNRFTLGELDGSTSARVQAISASLTKAGFKAPVMADIRSEIWLKLWGNLSFNPISA
                     LTHATLVDICRFPLSRDLAAQMMREAEAIANKLGVTFRVSIERRIAGAEKVGAHKTSM
                     LQDVEQGRPIEIEALLGSVVELGALTQTPTPHISAVYACASLLAQTLNAAHAGLSIGR
                     S"
     gene            3616..5199
                     /locus_tag="VD791_06260"
     CDS             3616..5199
                     /locus_tag="VD791_06260"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXV07613.1"
                     /translation="MHRISLAAVVLLFGCTTAHAAGEAAHIIQARAGQLANGGEVRVH
                     GASIASTVVLPAFYERRGFEPAWIDARNVDALLAAIRDSAADGLQPEDYHLSALEALR
                     GAPHGTVRDANFDLLATDALVRLGYHLRFGKVDVARIDPHWNFRRDYEAVLLLAPTVA
                     IQEALDQHRIGEALDVLRPGHPLYAALRGALQRYREIEAAGGWEPVPAGATLRPGATD
                     PRVPALRARLAAEGDLGEPDVESGEVYGAALESAVKRFQERHGLTPDALVGSRTQRAL
                     DVPVARRIDQLRLSLERSRLLMHDLPERFVVVNVPGYRVYYVEAGAVRFASDVVVGKV
                     MARTPIFRAEMTHLVINPSWTIPPIVMKTEILPGLEKDPDYLARNGLQRVDGQVVQPP
                     GPNNALGRIKLMFPNPHYVFLHDTPRKELFGRRDRTFSSGCVRVEDAFGLAEAVIGDP
                     QRWSRGQLLEAADTGETQTIKLERRVPVLLVYWTAGVAADGRVVFYEDVYGRDAAELQ
                     ALDAPFALHEKAVSAARRR"
     gene            5269..5757
                     /locus_tag="VD791_06265"
     CDS             5269..5757
                     /locus_tag="VD791_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXV07614.1"
                     /translation="MSLMQRIGGWVALGVRGVEFLTPVIDLALRLYVANVFFRSGLVK
                     IQSWTSTLSLFEYEYAVPLLSPAVAAYLGTAAELVLPVFLALGLGARLTALALFIFNW
                     IAVISYPDISDAGIKDHVLWGWLLAVAFFHGPGKLSMDHFIERWYRQRPAPPHGRASP
                     AS"
     gene            complement(5796..6557)
                     /locus_tag="VD791_06270"
     CDS             complement(5796..6557)
                     /locus_tag="VD791_06270"
                     /inference="COORDINATES: protein motif:HMM:NF022307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HXV07615.1"
                     /translation="MASGSGSPDHEQPVRSGLLLIADIAGYTRFMKAHRTSLAHAQDV
                     VGRLLEAMIDAVPALTLIEVEGDAAFLYRPLAASADGGVAVSAVEQARAMHRAFHVRQ
                     QGMVACNRCSCDGCTQTGNLRVKFVAHVGEVATQRVKALTRLAGFDVIVVHRMLKNSV
                     PVPEYVLLSDRLFANADDSLRERARALEQEFEGLGRARTYFLDLQEIATDLPPAPVAT
                     WWAKQRETWGVVLRSLPRVLGFVRGEPALRNLPTG"
     gene            complement(6701..7324)
                     /locus_tag="VD791_06275"
     CDS             complement(6701..7324)
                     /locus_tag="VD791_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXV07616.1"
                     /translation="MRRRILEAATAEFASAGYGGARVERISRSAATVDRMLYYYFGSK
                     EGLFRTVLEDAYQKLGAAEQRLELTDAEPVRGMRALVTFTWNYYLEHPEFIRLLNSEN
                     LYRGEHVRKSRRVQSLSFPLLSILTDLLARGKAQGKFRRGADPVQIYITVAALAYFYL
                     SNRYTLSRFLGYDLMAPAQRERWLRHITGVVLGSIVRPDAARRNRGN"
     gene            complement(7464..7787)
                     /gene="fdxA"
                     /locus_tag="VD791_06280"
     CDS             complement(7464..7787)
                     /gene="fdxA"
                     /locus_tag="VD791_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HXV07617.1"
                     /translation="MTHVVTEACIKCKYTDCVDVCPVDCFKEGPNMLIIDPDECIDCA
                     VCIPECPVNAIYADVDLPQDQLKFVELNAELARTFPTITDKKEAPPDADQWKDVKDKL
                     QYLER"
     gene            7985..8830
                     /locus_tag="VD791_06285"
     CDS             7985..8830
                     /locus_tag="VD791_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HXV07618.1"
                     /translation="MSESHYLQPSVLDDALAALAAAPWAVLAGGTDFYAARVGRPIRE
                     AILDISRLTPLRGIAHEAGGWRVGALTTWSDVLRQPLPPLFDGLKQAAREVGGVQTQN
                     AGTVAGNLCNASPAADGVPALMSLSAEVELASTRGVRRLPLTDFVTGNRATQRRPDEL
                     VTAILLPARSERARSRFLKLGARRYLVISIAMAAVTLDAGEDGKVAYAGVAVGACSPV
                     ARRIPALEARLTGRALNAELADLVRPADLGELSPISDVRGTAEYRREIALTLIRRALQ
                     ELADE"
     gene            8823..11636
                     /locus_tag="VD791_06290"
     CDS             8823..11636
                     /locus_tag="VD791_06290"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXV07619.1"
                     /translation="MNDLTRPQPGPAPELPAEAIGFELNGRPCAVSSPPAAPLSDVLR
                     DELGLTGTKVGCSAGDCGACTVLLDGEQVCSCLVAAAQVRGRRVTTVEGLAQVGRLTR
                     LQRAFLAHGAAQCGICTPGMLMAAADVLARNPAPTEQDVLDGLAGVLCRCTGYRKIVE
                     AVLASAGADIDSPQPAAGAAVGSRAAKPDGPPKVTGAEVYAADRVPEGALWLRVIRSP
                     HARARFRLGDLQPLRAGHPGLVAVFTARDVPVNSFGIFPQVKDQPVLADGEVRFRGEA
                     VAALVGGYDDVMAVQEHEVPIEWSPETPLADMDAALAAQGRPLHAFAPDNVLIRGRLV
                     KGDVDAALAAAAHVAQGEFTTSYVEHAYIEPEAGYAECMRDAAGGARLRIFACTQTPY
                     MDREEVARVLGLRAEQVHIVPSAVGGGFGGKLDIALQPLLALAAWKLGRPVRCVYTRP
                     ESMLSTTKRHPGRMRARFACDANGRLTAADFLGDFNTGAYASWGNTVANRVPIHASGP
                     YLVPNVRALTRAVYTNGPIAGAFRGFGVPQSTIVHEALLDELAEKVGMDRLELRLLNA
                     IRAGQATATGQMLRASAGLAECLRRLEPAWQAALSAVAGFNACHHALSGSRRGRRRGA
                     GVACMWYGIGNTVIANPSTMRVGLRRNGRLFLYNGAVDIGQGSNTILPQICADAVGLP
                     LALFDQVMGDTDLTADAGKSSASRQTFVSGNAARAAGEALRGKLLACLRVSAQAQLRI
                     VGTDLVALDRGTQKRLDLTALEADRNGDVAVGEGYFDPPTTPLDRDGQGVPYATYGFA
                     AQMAEVEVDLELGTVRVLKVHAAHDVGRAINPTQVEGQIHGGIAQGLGMALMEEYVAG
                     KTDNLHDYLIPTIGDVPPIVVHIVEHPEPLGPFGAKGVGEPALIATAPAIFNAIHHAT
                     GVRITHVPATPDRVRQAIAEQAR"
     gene            11633..>11902
                     /locus_tag="VD791_06295"
     CDS             11633..>11902
                     /locus_tag="VD791_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxynicotinate reductase"
                     /protein_id="HXV07620.1"
                     /translation="MSEPAAASSEGRPAAGKIECDACPVLCQISPGRTGACDRWGNFN
                     GKLTRVDPVVLFRKVSAQAGAITGFLEHKDWDGSAIADSPVFVSGV"
BASE COUNT         1772 a         4091 c         4164 g         1875 t
ORIGIN      
        1 gccgcggtcg gcggcggtga gcgccagcgt ccggcacacg ttgcgcgccg aggcgcacag
       61 gttgcgctgg gtgagcggta cgatcttcgg gcgcgacgtg gtgccggagg tgtgcagcac
      121 cagcgcgatg tccccctccg cggccggtcc gggcgaccgc gccggtttcg gttgcatgga
      181 gtccgggaac tcgagcgtga atacacccgc acccagctcc ggctgcggcg ccaagcgcac
      241 gaccgggacg cccagcttct gcgccatttc cagggcgggg gaagggcgac cgcgctcgac
      301 catcaggact ttggccccga ggtcgcgcag gtagaactcg aactcctcgg cccggtacgt
      361 agggttgagc ggcgccgccg ttgccccggc cgccacgcag atgaaggccg ccgccatctc
      421 cgggccgttg tcgagcacga tcgcgacgcg gtcgtttcga ccgatacccc tggcattgag
      481 cgcggcgacg gtgcgtgcga ccagcgcccg cagcgcgccg taagtgaggg gcacgccgcc
      541 gggtgcgctc agggctacgg catccgcccc gcccgcttcc agcagcccga tcagggtgac
      601 ggctgagttg ttcattcgac tccttgtccg cggcgaatta tcgacttcgc ctcgatcgcg
      661 cgcaaggagc ccccggttct gagatgatgg acgctcgcgc gcccacatca caggcaacag
      721 aatgcggatc gagccgattc cggccggcgg aacactcggc gccaccatcg aggggatcga
      781 tctcgcctgc gcgtccggga cggatgtgga taagttgatc gacgcgctcg gacgatacgg
      841 cgtgctgcgt ttcccgcgac agagcctctc ggcaacggac ctcaagaact tcagcgcacg
      901 actgggcgag ctggagatca acgtggcggg aacgttccag gaacccgggc atccgcaggt
      961 gatgatcctg tccaacatcg tcgagaacgg gcagccgatc ggccttgccg acgccggcca
     1021 gggctggcat gcggacatgt cttacagccg ggtggtcgca ttcgccaacg tgctctatgc
     1081 cgtcaaggtg ccgcagcacg gcggtcggcc gctcggagcc acccagttct gcgacatgtg
     1141 cgccgcgtac gacggcctgc cgaacgaaac caagcgccag ctggaaggaa agtcggcgct
     1201 gcacgacttc aacaagttct gggagacgat gcggcgccgg ccaggcagca cccggccgcc
     1261 gctcacggag gcgcagcgcc agaccaagcc acccgtgtcc catcccatcg tcctgaccca
     1321 tccgatcagc gggcgcaagg cgctgtacgc gaacccgggt tacaccacgc gcgtcgacgg
     1381 gttgccgcaa gcggagagcg atcggctgct ggagtttctg ttcgcgcatc agacccggga
     1441 cgagtaccgc tatgtattcc actggcaggt gggcgacgtg ctggtatggg acaacctgcg
     1501 cgtgattcac caagcggtgg ccgactacgg cccggaggag catcgcctga tgaagcgctg
     1561 tcaagtgatg gccacgcggt tttccccagg ctgatcgcat ggcgaagctg gcgtctgccc
     1621 tgatcctcgt cgggctgacg attgccgctg gcgccgcatg ggcgcaaacc taccccagtc
     1681 agccgatccg gatcatcgtc ccgttcggcc cgggaagcgg cagcgacatc gtcggccgcc
     1741 ggctcgggat ctatctgcag gagcgctgga agcaaccggt cgtcatcgac aaccggcccg
     1801 gtgtgcaggg gatgatcggc accgaagcgc tgagaagcgc tccggccgac ggctacacgt
     1861 tggcgatgtc gaccaattcc acgcacgccg ccgcactgca cctgatcaag aacctgccgt
     1921 acgatccgat cgcggacttc gaacacatcg ctctgatcgg taccaccggg tcggtagcgc
     1981 tcgtgcgccg cgacagcccg ttcaagtcga tcccggagct ggcggcgtat gccagggcgc
     2041 atcccgggaa ggtgttcttt ggccacgccg ataccatctc ccagattccc ggcgaactgt
     2101 tgcgcgcggc cgcaaagctt cccgtcgaag gtgtcgcgta caagacgagc gccaatgtca
     2161 tcgtcgatct gatcgggggt cagatccagt tcgccttctt caactacatg accggcgccg
     2221 cacaggccgc gagcgggcgg ctggtgccga tcgccatcac cgaatccagc cgcagttcgc
     2281 gctggcccgc ggtccccacc ttgagcgaaa cctatccggg cctcgaggtc accgccttcg
     2341 tcggcgttac cgctccgcac ggtgtcccgc cgcagatagt ggccaagctt catcgcgcca
     2401 ttcaggaggc gcaggacgac cccggattca gagagccgct cgagggcggg gggctcacct
     2461 tcgtgccgct gccgctcggc gggtaccgca gcttcatcct gcgggaaacg cagcggtggc
     2521 gcgagcacgt gaagacggcg ggtttggtcg cgcgctagct ccgtccaatc gacaatccgg
     2581 cgtgcgctgc gttcagcgtc tgcgcgagca ggcttgcgca cgcatacacc gcgctgatgt
     2641 gaggggtggg cgtctgcgtc agcgcgccca gctcgactac cgagccgagc agggcttcga
     2701 tctcgatcgg ccggccctgc tcgacgtcct gcagcatcga ggtcttgtgc gcgcccacct
     2761 tctccgcgcc cgcgatccgg cgttcgatcg acacgcggaa cgtcacgccc agcttgttgg
     2821 cgatggcttc ggcctcacgc atcatctgcg cggcgaggtc gcggctgagc gggaagcggc
     2881 agatgtccac cagggtggcg tgggtcaagg cgctgatcgg gttgaagctc aggttgcccc
     2941 acagcttgag ccagatctcg ctgcggatgt cggccatcac cggcgccttg aacccggctt
     3001 tggtcaggct ggccgagatg gcctgcacgc gcgcgctggt cgatccgtcc agttcgccca
     3061 gtgtgaagcg gttgccctcc accacctgta ccacgcccgg ggcgatcagg ttggccgccg
     3121 ggtagaccac gctgccgatg atgcgcgccg ggtcgatgtg acggcagatg ctgccgtcgg
     3181 gatcggcgct gtgcaccgga gtgccttcgt attgtccgcc gtgcctgtgg aaataccacc
     3241 acgggatgcc gttttgcatg gtgacgatgg cggtgtcggc atggcacaag tgccgcagct
     3301 cgggcgctat ggccgcgact tgatgcgcct tgacggtgag caggacagtg tcctgcgcgc
     3361 cggcctcggc cggcttctcc accgcgcgcg cgccgacgct gcgcacctcg gcgccgtcct
     3421 cgccgatcag ccgcatgccg tcggttcgga tcgccgccag gttggccccg cgcgcgatga
     3481 agacgacttc ctcgccggcg gccgacagcc tggccccgag atatccgccg atggcaccgg
     3541 cgccgacaac cgcgattcgc atggttccag tcacacctat aattgcccgc gctgcgaatt
     3601 atagacatgc cctcgatgca tcggatttcg ctggccgccg ttgtccttct gttcggctgc
     3661 accacggcgc acgccgcagg cgaggcggcg cacatcatcc aggcgcgcgc cgggcagctc
     3721 gccaacggcg gcgaggtgcg cgtccacggc gcgtcgatcg cgtccaccgt ggtgctgccg
     3781 gcgttctacg agcggcgcgg gttcgagccg gcctggatcg atgcgcgcaa cgtcgacgct
     3841 ctgctggctg cgatccgcga cagcgcagcc gacgggctgc agcccgagga ctatcacctg
     3901 tccgcgctgg aggcgctgcg cggcgcaccg cacggaacgg tgcgcgacgc caacttcgac
     3961 ctgctcgcca ccgacgccct ggtgcggctg ggctatcacc tgcgcttcgg caaggtggac
     4021 gtcgcgcgca tcgatccgca ctggaacttc cggcgcgatt acgaagccgt gctgctgctc
     4081 gcaccgaccg tcgcgatcca ggaagcgctg gaccagcatc gcatcggcga ggctctcgac
     4141 gtgcttcgtc ccggtcatcc gctgtacgcc gcgctgcgcg gcgcgctgca gcgctatcgc
     4201 gagatcgagg cggccggcgg gtgggagccg gttcccgccg gcgcgacgct cagacccggt
     4261 gcgacggacc cgcgcgtgcc ggcgctgcgg gcgcggctcg cggcggaagg cgatctgggg
     4321 gaacccgacg tcgagtccgg cgaggtgtac ggcgcggcgc tggaatccgc ggtcaaacgc
     4381 ttccaggaac gtcacggact gacgcccgat gcattggtcg gcagcaggac gcagcgtgcg
     4441 ctcgacgtgc cggtggcgag gcgcatcgat cagctgcgcc tgtcgctgga gcgcagccgg
     4501 ctgctgatgc acgacctgcc ggagcgcttc gtggtggtca acgtgcccgg ctaccgcgtc
     4561 tattacgtgg aggccggcgc ggtgcgcttc gcctccgacg tggtggtggg caaggtgatg
     4621 gccaggacgc cgatcttccg cgccgagatg acgcacctgg tgatcaaccc gtcctggacc
     4681 atacccccga tcgtcatgaa aaccgagatc cttccgggcc tcgagaagga ccccgactac
     4741 ctggcgcgca acggcctgca gcgggtggac gggcaggtag tgcagccgcc cggtccgaac
     4801 aacgcgctgg gccgcatcaa gctgatgttt cccaatccgc actacgtctt cctgcacgac
     4861 actccgcgca aagagctgtt cgggcggcgc gaccgcacct tcagcagcgg ctgcgtgcgc
     4921 gtggaggacg ccttcggcct ggccgaggcg gtgatcggcg atccgcagcg ctggtcgcgc
     4981 ggccagctgc tggaagcggc cgacaccggc gaaacgcaga ccatcaagct cgagcggcgc
     5041 gtgccggtgc tgctcgtcta ctggaccgcc ggcgtcgccg cggacgggcg cgtggtgttc
     5101 tacgaggacg tctacgggcg cgatgcggcc gagctgcagg cgctggacgc gccgttcgcg
     5161 ttgcacgaga aagccgtctc ggcggcgcgc aggcggtgac gacccggctg catcctcctg
     5221 cgccccggcc gcgtacatgc tggtggtgcg gccgtgggag gtgcagcgat gagcctgatg
     5281 cagcggatcg gagggtgggt ggcgctgggt gtgcgcgggg tggagttctt gacccctgtg
     5341 atcgatttgg ctctgcgcct gtacgtagcc aacgtgttct tcaggtcggg gctggtcaag
     5401 atccagagct ggaccagcac gttatccctg ttcgaatacg agtatgccgt gccgctgctg
     5461 tcccccgcag tggccgctta tctgggcacc gcagcggagc tggtgctccc cgtgtttcta
     5521 gcgctcgggc tcggcgctcg ccttacagcg ctcgcgttgt tcatcttcaa ctggatcgcg
     5581 gtgatctcct accccgacat cagcgatgcc ggcatcaagg atcatgttct gtggggatgg
     5641 ctgctcgcgg tcgcgttttt ccacgggccg ggaaagctct cgatggatca tttcatcgag
     5701 cggtggtacc ggcagcgccc agctccgccg catggacggg catcgccagc ttcctgactg
     5761 aactgttttc ttcgcctttc ccggccggaa ccggatcatc cggtcgggag gttgcgaaga
     5821 gctggctcgc cacgtacgaa gccgaggacc ctgggcagcg acctcagcac tacaccccat
     5881 gtttcccgct gctttgccca ccaggtcgcg acgggagctg gcggcaggtc ggtggcgatc
     5941 tcttgcaggt ccagaaaata cgtgcgcgcg cgccccaggc cctcgaactc ctgctcgagc
     6001 gctcgagccc gctcgcgaag ggagtcgtcg gcgttcgcaa acagccgatc cgacaacaac
     6061 acgtactcgg gcacagggac cgagttcttc agcatccgat gcactacgat cacgtcgaaa
     6121 ccggccagcc gtgtcaaggc cttcacgcgc tgggtcgcaa cctcacccac gtgtgcaacg
     6181 aacttcaccc tgaggttacc ggtctgcgtg cacccgtcgc acgagcaccg gttgcaggcg
     6241 accatcccct gctggcggac gtggaacgcc cgatgcatgg cccgggcctg ttccaccgct
     6301 gaaacggcca cgcccccgtc tgcgctggcc gccagcggcc gataaagaaa ggcggcgtcg
     6361 ccttccacct cgatcagggt caacgcgggc acggcgtcga tcatggcctc gagcagccgt
     6421 ccgacgacgt cttgtgcatg cgcaaggctg gtgcgatggg ctttcatgaa acgcgtgtag
     6481 cccgcgatgt cggcgatcag cagaagcccg ctgcgcaccg gttgctcgtg atcggggctg
     6541 cccgagccgc tcgccatcgg ttggtcctgc gccgcagtcg tattgcagca gtcggttaag
     6601 ttaagcgccc gccaggcact ctgtcaaata cgtccgattg ggcgcggcgg ttgcggttag
     6661 cggcctggcc gacccggtta cgacgtgccg ccgcgcaagc tcaattcccc cgattgcgcc
     6721 gcgccgcatc cgggcggacg atgcttccca ggaccacgcc ggtgatgtgc cgcagccagc
     6781 gctcgcgctg cgcgggcgcc atcaggtcgt agcccaggaa gcgcgacagc gtgtagcgat
     6841 tcgacaggta gaagtaggcc agcgccgcca ccgtgatgta gatctgcacc gggtcggcgc
     6901 cgcgccggaa cttgccctgc gccttaccgc gcgcgagcag gtcggtgagg atcgacagca
     6961 gcgggaagga cagcgactgc acgcgccgcg acttgcgcac gtgctccccg cggtacaggt
     7021 tctcgctgtt caacagccgg atgaactccg ggtgctcgag gtagtagttc caggtgaagg
     7081 tcaccagcgc gcgcatgccc cgcaccggct ccgcgtcggt gagctccagc cgctgctccg
     7141 cggcgcccag cttctggtag gcgtcctcca gcacggtgcg gaacagccct tccttgctgc
     7201 cgaagtagta atacagcatg cggtccacgg tggcggcgct gcggctgatg cgctccaccc
     7261 ttgcgccgcc gtagccggcg ctggcgaact ccgcggtagc ggcctccagg atgcgccgcc
     7321 gcatggcctg cggatcgcgc gtacgctgtg ccggcttgcg ccctgggtgg ccgggggggc
     7381 ggccgggagg gcggcctggg agccggccgc ctgcggagcg cgcgcgtctc cgggggcggg
     7441 ctggcgctcg tgccgccaac gcctcagcgc tccaggtact gcagcttgtc cttcacgtcc
     7501 ttccactgat ccgcgtcggg cggcgcttcc ttcttgtccg tgatcgtggg aaaggtcctg
     7561 gcgagctcgg cgttgagctc gacgaacttg agctggtcct gcggcaggtc aacgtcggcg
     7621 tagatggcgt tcaccgggca ctccgggatg cacaccgcgc agtcgatgca ttcgtcggga
     7681 tcgatgatca gcatgttcgg gccttccttg aagcagtcca ccgggcagac gtcgacgcag
     7741 tcggtgtact tgcacttgat gcaggcctcg gtgacgacat gggtcatgga tgctcctgga
     7801 aggggcccgg cttgcggccg gcgtgcgagt aaagcgtttt cttcatacaa tggagcaacc
     7861 gctccatagc gggggtacgc gccttgcttt cccggtggtc atgaggcgtc cccgcggggc
     7921 gaaagcatag caggacggcc ggtcttccgg caccgtccgc cgcccggctg gcccgaggag
     7981 agccgtgtcc gaatcgcact acctgcagcc ttccgtgctc gacgacgcgc tggcggcgct
     8041 cgccgcggcg ccctgggcgg tgctggccgg cggcaccgac ttctacgccg cccgggtggg
     8101 ccgcccgatc cgcgaggcga tcctggacat cagccgcctg acacccctgc gcggcatcgc
     8161 gcacgaggcc ggcggctggc gcgttggcgc gttgaccacc tggagcgacg tcttgcgcca
     8221 gcccctgccg ccgttgttcg acggcctgaa gcaggcggcg cgcgaggtcg gcggcgtcca
     8281 gacgcagaac gccggcaccg tggcgggcaa cctgtgcaac gcttcgcccg ccgccgacgg
     8341 cgtcccggcc ctgatgagcc tgtcggcgga ggtggaactt gcctctacgc gcggggtgcg
     8401 ccgcctgccg ctgaccgact tcgtcaccgg caaccgggcc acacagcgcc ggccggacga
     8461 gctggtgacc gcgatactcc tgcccgcgcg cagcgagcgc gcgcgttcga ggttcctgaa
     8521 gctcggcgcg cgccgctacc tggtgatctc gatcgcgatg gcggccgtga cgctggatgc
     8581 aggggaggat ggcaaagtcg cttacgcagg cgtcgcggtg ggcgcgtgct cgcccgtcgc
     8641 acgccggata cccgcgctcg aagcgcggct taccgggcgc gcgctgaacg cagagttggc
     8701 ggacctggtc cgcccggcgg atctcggcga gctgtcgccg atctccgatg tgcgcggcac
     8761 ggcggagtat cgacgcgaga tcgctttgac cctgatccgg cgcgcgctcc aggagctggc
     8821 cgatgaatga tctgacgcgc ccgcagcccg gcccggcgcc cgagctgccg gccgaggcca
     8881 tcggtttcga gctgaacgga aggccctgtg cggtctccag cccgccggct gcgcccctgt
     8941 ccgacgtgct gcgcgacgaa ctcgggttga ccggcaccaa ggtcggttgc agcgcgggcg
     9001 attgcggtgc ctgcacggtg ctgctggacg gcgagcaggt ttgctcctgc ctggtggccg
     9061 ccgcgcaggt gcgcggacgg cgcgtgacca cggtcgaggg cctggcgcag gtggggcggc
     9121 tgacgcgact gcagcgggcg ttcctggcgc acggcgccgc gcagtgcggc atctgcaccc
     9181 cgggcatgct gatggcggcg gcagacgtgc tggcgcgcaa cccggcgccc accgagcagg
     9241 acgtgctcga cggcctggcc ggcgtactgt gccgctgcac gggctatcgc aagatcgtgg
     9301 aggcggtgct ggcgagcgcg ggcgcagaca tcgacagccc gcaacccgct gcgggcgcgg
     9361 ccgtgggcag ccgcgctgcc aagccggacg gcccgccgaa ggtcaccggc gccgaggtct
     9421 acgccgccga ccgggtgccg gaaggcgccc tgtggctgcg cgtgatccgc tcgccccacg
     9481 cccgtgcgcg cttccggctg ggcgacctgc agccgctcag ggctgggcat ccggggctcg
     9541 ttgccgtgtt caccgcgcgc gacgtgccgg tcaacagctt cggcatcttc ccgcaggtga
     9601 aggaccagcc ggtgctggcc gacggcgagg tgcgcttccg cggcgaggcg gtggcggccc
     9661 tcgtgggcgg ctacgacgat gtcatggcgg tccaggagca cgaggtgccg atcgagtggt
     9721 ccccggagac gccgctcgcg gacatggacg ccgcgcttgc ggcgcagggc aggcccctgc
     9781 acgcgttcgc gcccgacaac gtgctgatcc gcgggcgctt agtcaagggg gacgtggacg
     9841 cggcgctggc cgcggcggcg cacgtggcgc aaggcgagtt caccaccagc tacgtggagc
     9901 acgcctacat cgagcccgaa gccggctacg ccgaatgcat gcgtgacgcc gccggcggcg
     9961 cccggttgcg gatcttcgcc tgcacgcaga ctccgtacat ggatcgcgag gaggtggcgc
    10021 gcgtgctcgg cctgcgcgcg gagcaggtgc acatcgtgcc ctccgcggtg ggcggcggct
    10081 tcggcggcaa gctggacatc gcgctgcagc cgctgctggc gctcgcggcg tggaagctgg
    10141 gccgcccggt gcgctgcgtt tacacccggc ccgagtccat gctgtccacc accaagcgcc
    10201 atcccgggcg catgcgcgcg cgcttcgcgt gcgacgcgaa cggccggctg accgccgccg
    10261 acttcctggg cgacttcaac accggcgcct acgcctcctg gggcaatacg gtggccaacc
    10321 gggtgccgat ccacgcctcg gggccctatc tggtgcccaa tgtgcgcgcg ctcacgcgcg
    10381 cggtgtatac caacggaccc atcgccggcg cgttccgcgg cttcggcgtg ccgcagtcca
    10441 ccatcgtgca cgaggccctg ctcgacgagc tggcggagaa ggtcgggatg gaccgtctgg
    10501 agctgcgcct gctcaacgcg attcgcgccg ggcaggcaac cgccaccggg cagatgctgc
    10561 gcgccagcgc cgggctcgcc gaatgcctgc ggcggctcga gcccgcctgg caggcggcgc
    10621 tgtccgccgt ggccggcttc aacgcctgcc accacgcctt gagcggctcg cgccgaggca
    10681 ggcgcagagg cgcaggggtg gcctgcatgt ggtacggcat cggcaacacc gtgatcgcga
    10741 acccgtccac catgcgcgtg gggctgcggc gcaacgggcg cctgttcctg tacaacggcg
    10801 cggtggacat cgggcagggc tcgaacacca tcctgccgca gatctgcgcc gacgccgtcg
    10861 ggctgccgct ggcgctgttc gaccaggtga tgggcgatac cgatctgacg gcggatgccg
    10921 gcaagtcctc ggcctcgcgc cagaccttcg tatcgggcaa cgccgcccga gccgcgggcg
    10981 aggcgctgcg cggcaagctg ctggcgtgtt tgcgcgtctc ggcgcaggca cagctgcgca
    11041 tcgtcgggac ggacctggtg gcgctcgacc gcggcacgca gaagcgcctc gacctgaccg
    11101 cgctggaagc cgaccgcaac ggcgacgtcg cggtgggcga gggctacttc gacccgccca
    11161 ccacgccgct ggatcgcgac ggccagggcg tgccctacgc gacctacggc ttcgccgcgc
    11221 agatggcgga agtggaagtg gatctggagc tggggacggt gcgggtgctc aaggtgcacg
    11281 cggcgcacga cgtggggcgc gccatcaacc cgacccaggt cgaggggcag atccacggcg
    11341 gcatcgccca gggcctgggc atggcgctga tggaggagta tgtcgccggc aagaccgaca
    11401 acctgcacga ctatctcatc cccaccatcg gcgacgttcc gccgatcgtg gtgcatatcg
    11461 tcgagcaccc cgagccgctg ggtccgttcg gcgccaaggg cgtgggcgag ccggccttga
    11521 tcgccaccgc ccccgcgatc ttcaacgcca tccaccatgc caccggtgtg cgcatcaccc
    11581 acgtgcccgc cacgccggat cgggtgcgcc aggccatcgc cgagcaggcg cgatgagcga
    11641 gccggccgca gcgtcgagcg aagggcggcc ggccgcgggc aagatcgagt gcgacgcctg
    11701 cccggtgctg tgccagatca gccccggccg caccggcgcc tgcgaccgct ggggcaactt
    11761 caacggcaag ctcacgcggg tggacccggt ggtgctgttc cggaaggtga gcgcgcaggc
    11821 gggcgcgatc actgggttcc tggagcacaa ggactgggac ggcagcgcca ttgccgattc
    11881 cccggtgttc gtatccggcg tg
//
LOCUS       DASPGE010000196         5267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_638495, whole genome shotgun sequence.
ACCESSION   DASPGE010000196 DASPGE010000000
VERSION     DASPGE010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638495"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VD791_06300"
     CDS             <1..572
                     /locus_tag="VD791_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HXV07621.1"
                     /translation="TIIRDEHRSLGAVVHAMQFLLREIRDRGLAPDYRLLHAMLYYIR
                     EFPERLHHPKEDRHLFAAVKRRTHEADAVIAELEREHAQGESLLNDLTVALSVYEAGV
                     PNGLERFAASAEKFADFYWQHMQKEEDRVLPVAERVLTDEDWHEIHAAFSVNRDPNYA
                     ADGEEEFRRLFTRIVNLAPAPIGLGAAQG"
     gene            652..1239
                     /locus_tag="VD791_06305"
     CDS             652..1239
                     /locus_tag="VD791_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV07622.1"
                     /translation="MKLKTLFATLALCAPMGALAAGLPGLRGSDHLGITVPKLDQAVD
                     FFVNVIGCEAFYKMGPFKADDDWMQVHLDVNPRAEIPSMRLVRCGYGVNLEIFEYSVP
                     EQSKAPPKNSDIGGHHLAFYVDDMAKAVAHLKANGVKVLGEPTVMGGGPTAGETWVYF
                     MAPWGMQLELVSYPKGKAYEKEYKSRLWSATNPAK"
     gene            1239..2489
                     /locus_tag="VD791_06310"
     CDS             1239..2489
                     /locus_tag="VD791_06310"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HXV07623.1"
                     /translation="MARIRRLLAGLLWAVALLGGATAHAHFPAGYDLRAVHFEHAGDG
                     LHAYFRLTLPLVVAAGLGPKRADGNHEPAPFTVLRIESNHGFYYPDVARIRAEPLGLG
                     RLIADGHRLETDTAVLPPQVRAVRVYPKGHVPPFNTLAEVKSATAPGEVYPQAALDTD
                     VAYVAVDAHLVYPASNAPFRLSSTLLNRVIGQPEVQNLLVDHRDAPVVYRRTGLLDAP
                     IEINPSAWSAAATFVREGVAHILHGADHLLFVLCLTLASATLVQLAWRVTGFTVGHTV
                     TLIAGFFGIAPQVSWFVPAIETAIAVSIALAALLLLRRGSGRVLMPGLTVAIGLIHGF
                     GFSFSLREMLQLEGPHLWLSLLSFNVGVEIGQIAVAAVVWLLAVAALQHAGSWQMRVR
                     AGVALGCTAVSLFWIYERGTALLA"
     gene            2575..3132
                     /locus_tag="VD791_06315"
     CDS             2575..3132
                     /locus_tag="VD791_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07624.1"
                     /translation="MTTPETYLATQPAHYIAYAPRGPGLECAVVYFVEGDHVYGWWTG
                     FKDYQYASAFFKLENFFSPRETVFLATEGSDIYGGWRYLYSKREPRLDKPIPVDDDIC
                     HKLESLQDAFANEWLWFRGGPGSEAEASAYEQAELAVQDVNIKHRRLGKLHKRAPVWT
                     GESHGLNLDIMDYLAEHWPLDYGKS"
     gene            3179..4909
                     /gene="polX"
                     /locus_tag="VD791_06320"
     CDS             3179..4909
                     /gene="polX"
                     /locus_tag="VD791_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HXV07625.1"
                     /translation="MAVTNATIAQIFDEIADLLDIQSANPFRVRAYRNAARTIGELGT
                     DVKTLMARGMPLTDIPGIGADLSNKITEIVETGSCEFLKRLEKDVPPAVTQLMRLPGL
                     GPKRVKTLWKDLDIQTMDQLLKAARAGRIRELPGFGEKTEQKILEAAQRQLAQAPRVK
                     LAVAAQYGEPLTAYLRAVPGVLRVDITGSYRRMRETVGDLDLVVVAEDSAEVMERLAR
                     YEEVREVQASGPTRSTVILKSGMQVDVRVVPEESYGAALQYFTGSKAHNIAIRRMAQD
                     KGLKINEYGVFRGDKRIAGKTEASVYKTVGLPWIPPELREDRGEIEAARQGTLPTLIE
                     LADLKGDLHAHSKASDGHHTIEEMALAAKARGLEYLAITEHSKRLTVARGLDAVRLAK
                     QGDEIDRLNARLEGITVLKGIEVDILEDGTLDLPDSVLSKLDVVIAAVHSRFELPRPK
                     QTARILKALDNPRVHLLAHPTGRLIEAREPYDVDMQQIIRKAKARGVALEVNAHPQRL
                     DLSDTFCRMAKDEGALLSINSDAHSTFELDMLRFGLGQARRGWLEAKDVLNARPLKAL
                     HKLLAARVAR"
     gene            4948..>5267
                     /locus_tag="VD791_06325"
     CDS             4948..>5267
                     /locus_tag="VD791_06325"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07626.1"
                     /translation="MEEQLQTLDQVKSTAIDLAMHFGPKLLVAILIMAIGVFVGRWVG
                     RFVHKGLVKFHLEPPVRELLVRILRVLILGIFLLMALQNLGVELLPLIAGLGVAGAGI
                     ALAM"
BASE COUNT          886 a         1791 c         1787 g          803 t
ORIGIN      
        1 caaccatcat ccgcgacgag caccgctcgc tcggcgcagt cgtgcacgcg atgcagttcc
       61 tgctgcgcga gatccgcgac cgcggtctcg cacccgacta ccgcctgctg cacgccatgc
      121 tctactacat ccgcgagttc cccgagcgcc tgcaccatcc caaggaggac cggcacctgt
      181 tcgccgcggt caagcgccgc acgcacgaag cggatgcggt gatcgcggag ctcgagcgcg
      241 agcatgcgca gggcgagagc ctgctcaacg acctgaccgt ggcgctgagc gtgtacgagg
      301 ccggcgtccc gaacggcctg gagcgcttcg ccgcgagcgc tgagaagttc gccgatttct
      361 actggcagca catgcagaag gaggaggatc gcgtgctgcc ggtcgccgag cgcgtcctca
      421 ccgacgagga ctggcacgag atccacgccg ccttcagcgt caaccgcgat cctaactacg
      481 cggcagacgg cgaagaggag tttcgccggc ttttcacgcg catcgtcaac ctcgcgcccg
      541 cgcccatcgg actgggcgcg gcgcagggct gacgcggccg ggagggcgcg gctcaccgcg
      601 cgcgccgcca ggcgcgcatt caccagcacg tcaccgggag gagtacaaga aatgaagctg
      661 aagacgctgt tcgctacgct ggcattgtgc gcgccgatgg gcgctctggc ggccgggctg
      721 cccggtctgc gcgggtcgga ccacctcggc atcaccgttc cgaagctcga ccaggcggtg
      781 gacttcttcg tcaacgtcat cggctgcgag gccttctaca agatgggccc gttcaaggcc
      841 gacgacgact ggatgcaagt gcacctcgac gtcaatccgc gcgccgagat cccgagcatg
      901 cggctggtgc gctgcggcta cggggtgaac ctggagatct tcgagtacag cgtgcccgag
      961 cagagcaagg caccgcccaa gaacagcgac atcggcggcc accatctggc gttctacgtg
     1021 gacgacatgg ccaaggcggt ggcgcacctc aaggcgaacg gcgtgaaggt gctcggcgaa
     1081 ccgacggtga tgggcggcgg gccgaccgcg ggcgagacct gggtctattt catggcgccc
     1141 tggggcatgc agctcgagct ggtgagctat cccaagggca aggcctacga gaaggaatac
     1201 aagtcccggc tgtggagcgc gaccaatccg gccaagtgat ggcgcggatt cgccgcctgc
     1261 tggcgggttt gctgtgggcg gtcgcgctcc tcggcggcgc gaccgcgcac gcccactttc
     1321 ccgccggcta tgacttgcgt gcggtgcact tcgagcacgc cggcgacggc ctgcacgcct
     1381 atttccgcct gacgctgccg ctggtcgtcg ccgccggcct gggccctaag cgcgcggacg
     1441 gcaaccacga gccggcgccg ttcacggtgc tgcgcatcga gagcaatcac ggcttctact
     1501 atcccgacgt cgcgcgcatc cgcgccgagc cgctggggct cggcaggctc atcgccgacg
     1561 gccaccggct ggaaacggat actgccgtgc tgccgccgca ggtgcgcgcc gttcgcgtct
     1621 atccgaaggg ccacgtgccg cctttcaaca cgctggccga ggtcaagtcg gccaccgcgc
     1681 ccggcgaggt ctatccgcag gccgcgctcg acaccgatgt cgcgtacgtc gcggtggacg
     1741 cgcacctggt gtatcccgca tcgaacgcac ccttccgctt gagcagcacg ctgctcaacc
     1801 gcgtgatcgg acagcccgag gtgcagaacc tgctggtgga tcaccgcgac gcgccggtcg
     1861 tttaccgcag gacggggctg ctggatgccc cgatcgagat caacccttcg gcctggagtg
     1921 ccgcagcgac cttcgtgcga gagggcgtcg cccacatcct gcacggagcg gaccacttgc
     1981 ttttcgtgct ctgtctgacg ctcgcctcgg cgaccctggt gcagctcgcc tggcgcgtca
     2041 ccggcttcac cgtggggcac acagtcacgc tgatcgccgg cttcttcggg attgcgccgc
     2101 aggtgtcctg gttcgtcccg gccatcgaga ccgccatcgc ggtgtcgatc gcgctggccg
     2161 cgctgctgct gctccggcgc ggcagcggcc gcgtgctgat gccgggactg accgtggcga
     2221 tcggcttgat ccacggcttc ggcttctcgt tctcgctgcg cgagatgctg cagctcgagg
     2281 ggccgcacct ttggctgagc ctgctctcct tcaacgtggg ggtggagatc ggccagatcg
     2341 ccgtcgcggc cgtggtgtgg ctgctggcgg tggccgcgct ccagcacgcc ggctcgtggc
     2401 agatgcgcgt ccgggccggg gtcgcactcg gctgcaccgc cgtgagcctg ttctggatct
     2461 acgagcgcgg gaccgcgctg ctcgcctgac ccgccgttga tctgagtcaa gcgccgccgg
     2521 ccgtcccggc gctaggctgg ctcggatgcg cgccattgcg ctcgaggcct tgccatgacc
     2581 acccccgaaa cctatctcgc gacccagccc gcgcattaca tcgcctacgc cccgcgcgga
     2641 ccggggctgg agtgtgccgt ggtgtacttc gtcgagggcg accacgtgta cggctggtgg
     2701 accgggttca aggactacca gtacgcgtct gcgttcttca agctggagaa cttcttctcg
     2761 ccgcgcgaaa ccgtgttcct cgccaccgag gggtcggaca tctacggcgg ctggcgctac
     2821 ctgtactcga agagggagcc gcggctggac aagccgatcc cggtggacga cgacatctgc
     2881 cacaagctgg aaagcctgca ggacgccttc gccaacgaat ggctgtggtt ccgcggcggc
     2941 ccgggaagcg aggccgaagc cagcgcctac gagcaggccg agctggcggt gcaggacgtc
     3001 aacatcaagc accggcggct gggcaagctg cacaagcgcg cgccggtgtg gaccggggag
     3061 tcgcacggcc tgaacctgga catcatggac tacctggccg agcactggcc gctggactac
     3121 ggcaagagct gaagccgggc cgacaagagg cgggcggccg gtgtgctaag ttgcgcgcat
     3181 ggccgtcacc aacgccacca tcgcgcagat cttcgacgag atcgccgatc tgctcgacat
     3241 ccagagcgcc aatccgttcc gcgtgcgcgc ctaccgcaac gccgcccgca ccatcggcga
     3301 gctgggcacg gacgtgaaga cgctgatggc gcgcggcatg ccgctcaccg acatccccgg
     3361 catcggcgcg gacctgtcga acaagatcac cgagatcgtc gagaccggca gctgcgagtt
     3421 cctcaagcgc ctggagaagg acgtgccgcc cgcggtcacg cagctcatgc gcctgccggg
     3481 actgggaccg aagcgcgtca agacgctgtg gaaggacctc gacatccaga ccatggacca
     3541 gctgctgaag gcggcgcggg ccggtcgcat ccgcgagctg cccggtttcg gcgagaagac
     3601 cgagcagaag atcctggagg ccgcgcagcg ccagctggct caggctccgc gcgtcaagct
     3661 ggccgtggcc gcacagtatg gcgagcccct caccgcgtat ctgcgcgccg ttcccggcgt
     3721 gctgcgagtc gacatcaccg gcagctaccg gcgcatgcgc gagaccgtcg gcgatctcga
     3781 cctggtggtg gtcgccgagg acagcgccga ggtcatggag cggctggcgc gctacgagga
     3841 ggtgcgcgaa gtgcaggcga gcggcccgac gcgctccacc gtaatcctga agtcgggcat
     3901 gcaggtcgac gtgcgcgtgg tccccgaaga aagctacggc gccgccctgc agtacttcac
     3961 cggctcgaag gcgcataaca tcgcgatccg gcgcatggcg caggacaagg gcctcaagat
     4021 caacgagtac ggggtgttcc gtggcgacaa gcgtatcgcc gggaaaaccg aggcgtcggt
     4081 gtacaagacc gtgggcctgc cctggatccc gccggagctg cgcgaagatc gcggcgagat
     4141 cgaagcggcg cgccagggca ccttgccgac cctgatcgag cttgcggacc tcaagggcga
     4201 tctgcacgcc cactccaaag cgagcgacgg gcaccacacg atcgaagaga tggcgctcgc
     4261 ggcgaaggcg cgcggcctcg agtacctggc gatcaccgaa cactccaagc gcctcaccgt
     4321 cgcgcgtggc ctcgacgcgg tgcgcctggc caagcagggc gacgagatcg accggctcaa
     4381 cgccaggctc gaaggcatca ccgtgctcaa aggcatcgag gtggacatcc tggaggacgg
     4441 cacgctcgac ctgcccgact cggtgctgtc caagctcgac gtggtgatcg ctgccgtgca
     4501 cagccggttc gagctgccgc ggccgaagca gaccgcgcgc atcctcaagg ccctggacaa
     4561 cccgcgcgtg cacctcctcg cccatccgac tggccggctt atcgaggcgc gcgagcccta
     4621 cgatgtggac atgcagcaga tcatccgcaa ggccaaggcg cgcggcgtcg cgctggaagt
     4681 caatgcacat ccgcagcggc tcgacttgag cgacaccttc tgccggatgg ccaaggatga
     4741 gggcgcgctg ctgtcgatca actcggatgc gcacagcacc tttgagctcg acatgctgcg
     4801 cttcggcctc ggccaggcgc gccgcggctg gctggaggcg aaagacgtgc tcaacgcgcg
     4861 gccactgaag gcgctgcaca agctgctcgc ggcccgcgtc gcgcggtagc gctacaatcg
     4921 cccggccgtc gaaaaaaggg gaacgccatg gaagaacaac tgcaaacgct cgaccaagtg
     4981 aagagcaccg ccatcgacct cgccatgcac ttcggaccga agctgctagt ggcgatcctg
     5041 atcatggcca tcggcgtatt cgtgggacgc tgggtgggca ggttcgtgca caaggggctg
     5101 gtcaagttcc acctggagcc gccggtacgc gagctgctgg tgcgcatcct gcgcgtcttg
     5161 atcctgggca tcttcttgct gatggcattg cagaacctgg gggtggagct gctgccgctg
     5221 atcgcgggcc tgggcgtggc gggcgccggc atcgcccttg ccatgca
//
LOCUS       DASPGE010000197         5655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_873758, whole genome shotgun sequence.
ACCESSION   DASPGE010000197 DASPGE010000000
VERSION     DASPGE010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873758"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..561)
                     /locus_tag="VD791_06330"
     CDS             complement(115..561)
                     /locus_tag="VD791_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07627.1"
                     /translation="MRKTLIASALAAAFGFGVSCAMAQNADLQDTEDAKQKPTEQEGA
                     ATHTPDTKGSETTPRANQPGDAAGGAQTGNEEAADPAKQQSDIPDQSQGGVGHPEQEQ
                     TTGQGQGVKESGGAKQPDTGMPEQSKDTTPERDDATGQGDGVGEQR"
     gene            701..1807
                     /gene="tal"
                     /locus_tag="VD791_06335"
     CDS             701..1807
                     /gene="tal"
                     /locus_tag="VD791_06335"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HXV07628.1"
                     /translation="MASIAELNRHGQSIWLDYIDRNLLDNGGLQRLLDGGVSGVTTNP
                     TIFHKAITGSRDYDDSIRELLERDHEIGPVGLCEELMVADVQAAADALRPVFDRTDGR
                     DGYVSLEVPPDLAHDEARTVATAHRLRDAVGRPNVLIKVPGTVEGVRAFERLTAEGVS
                     VNVTLLFAVQRYEEVVRAWARGLARNRDPQRIASVASFFVSRVDSKVDKRLDALATPG
                     AQRLKGRIAIANARLAYRRFRELMAEADIARQLARGARPQRPLWASTSTKNPAYRDVL
                     YVESLIGPDTVNTVPPETLDAFVYHGEARATLEQDVEAAQRDIAALAELGIDLAAVTR
                     ELESEGVAAFRDSWDKLLASLKDKCFAVAKDFAR"
     gene            complement(1821..2960)
                     /locus_tag="VD791_06340"
     CDS             complement(1821..2960)
                     /locus_tag="VD791_06340"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV07629.1"
                     /translation="MDYRIISADDHIDMQWLPKDLWQKRVPAAWRERAPKVVDTADGP
                     YWVCGDDRWESWGGRKGAAGAMGGRRLALERGGVLEPGVLRPTTTALRLADMDRDGVD
                     ATVMYGPIVPLLIADPALRQVCYRAYNEWLAEFCASASERLVGAGLVPIDDPRMAAEE
                     VRHLRRLGIRTGMFLAARVELPLWDQAWTPLWEAAAETGIPIGFHLGGGHRTVVWSGP
                     KASDAGNMGVRVSCSTLQMDEALAAVIFSGALERYPGLNIVLAETGIGWLPYLLERMD
                     DSYRKFLDAEEWWRVHGGLALRMPPSHYFRRQVWATFQTDHAGLRLLDLLGEERVMWA
                     SDYPHADSTWPESQRAIEENFHGVPARARARILCDNARALYGLGR"
     gene            3133..3741
                     /gene="ccmA"
                     /locus_tag="VD791_06345"
     CDS             3133..3741
                     /gene="ccmA"
                     /locus_tag="VD791_06345"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HXV07630.1"
                     /translation="MKLACARGGRPLFGELSFDLEPGELFWVTGPNGSGKTSLLRMLC
                     TLLPPESGEVRWRGHPVRALGESYRAELAYLGHAPAVKDDLSALENLRFALAQQGTAP
                     AERELLGALEAFGLAQREDLPLRSLSQGQKRRVALARLAFCAQRALWILDEPLTALDS
                     AAVELVRGHVEAHLARGGLVVLTSHQDVDLSRMRVQRLRLDA"
     gene            3858..4478
                     /gene="ccmB"
                     /locus_tag="VD791_06350"
     CDS             3858..4478
                     /gene="ccmB"
                     /locus_tag="VD791_06350"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HXV07631.1"
                     /translation="MRRRSDVLTTFFFFIIVVSLFPLGVGPEPGTLREIAPGVVWVAA
                     LLASMLALTRLFAGDYADGTLEQLALVPQPLVLLVLAKIAAHWLVTGLPLVVIAPLLG
                     LQFDLPPDALAVMTAALVLGTPVLSMIGAVGAALTLGVRGAGGLLALLVLPLYVPVLI
                     FGAGAVTASAAGLSAAGHLSLLGAVLLLSLVFAPWATAAAIRIALE"
     gene            4483..5229
                     /gene="ccmC"
                     /locus_tag="VD791_06355"
     CDS             4483..5229
                     /gene="ccmC"
                     /locus_tag="VD791_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HXV07632.1"
                     /translation="MAVNLWRFASPQVFYPLAGRMLPWFFGAAVLFALAGLYVGLFVA
                     PTDFQQGEAYRIIFVHVPAAWMSMFLYLVMAFWCAVGLAMNTRLSFMMALALAPTGAL
                     MTFLALWTGSLWGKPTWGTWWVWDARLTSELILLFLYIGFLSLHAAIDDVRRADKAAA
                     LLALVGVVNVPIIYFSVKWWNTLHQGASVSLTRAPSMAATMLTGMLLMALAAWMYSIA
                     ASLMRVRAIILEREADADWVRALDEGPVPE"
     gene            5252..5431
                     /gene="ccmD"
                     /locus_tag="VD791_06360"
     CDS             5252..5431
                     /gene="ccmD"
                     /locus_tag="VD791_06360"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HXV07633.1"
                     /translation="MIWRSAAEFFAMGGYAVYVWGSYGVAACLLAAELWLLARRRRTL
                     LRRLGRSSTPREQHR"
     gene            5428..>5655
                     /locus_tag="VD791_06365"
     CDS             5428..>5655
                     /locus_tag="VD791_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HXV07634.1"
                     /translation="MKPRHKRLALIGAGIALLGGAAFLILNAFQSNLVFFYSPSQVAA
                     HEAPQGRAFRIGGMVQTGSVQREADGVTVRFT"
BASE COUNT          727 a         1940 c         2016 g          972 t
ORIGIN      
        1 tcccaccctc tccgccagca cgcgctgcgc gcgtggcgtc gagggtggtt cttcaacctc
       61 gaatcgatgc gctcacgctc gcttgcgagc gtgagacgac tcgatgaact gcgcttacct
      121 ctgttcgccc acgccgtcgc cctggccggt ggcgtcgtct cgttccgggg tggtgtcctt
      181 gctctgctcg ggcatcccgg tgtcgggttg cttggcgccg cccgactctt tcacgccctg
      241 accctggccc gtggtctgct cctgctccgg atggcccacg ccgccttggc tctggtcggg
      301 aatatccgat tgctgtttgg cggggtccgc cgcctcttca ttgcctgtct gggcgccgcc
      361 ggcagcatcg ccgggctggt tggcccgggg cgtggtttcc gatcccttgg tatccggcgt
      421 gtgcgtcgcg gccccttctt gttctgtggg cttttgcttg gcgtcttcgg tgtcctgcaa
      481 gtccgcgttc tgcgccatgg cgcagctcac tccgaacccg aatgcggcgg ccaatgcgct
      541 tgcgatcaac gttttcctca tggtgatctc ctgggtgggt tgacacgtca agcgcagcaa
      601 ctgctgtgcc gtcgcgtcca aggcgctgtt gggcgggccg tggttgctct ggcctacggg
      661 gatcggcgaa aatatcttgg atcaccaaac ggaggtgcgc gtggcatcga tcgcggagct
      721 caaccggcac gggcagtcca tctggctcga ctacatcgac cgcaatctgc tggacaacgg
      781 cggcctgcaa cggctgctgg acggcggggt gtcgggcgtc accaccaacc cgaccatctt
      841 ccacaaggcg atcaccggca gccgcgacta cgacgactcg atccgcgagc tgctggagcg
      901 cgaccacgag atcggcccgg tggggctgtg cgaggagttg atggtggcgg acgtgcaggc
      961 cgcggccgac gccctgcggc cggtgttcga ccgcaccgat ggccgcgacg gctacgttag
     1021 cctcgaggtg ccgcccgatc tcgcccacga cgaggcgcgc acggtggcca ccgcgcaccg
     1081 cttgcgcgac gccgtcgggc ggcccaacgt cctgatcaag gtgccgggca ccgtcgaggg
     1141 cgtgcgcgcg ttcgagcgtc ttaccgccga aggcgtcagc gtcaacgtga cgctgctgtt
     1201 cgcggtgcaa cgctacgaag aggtggtgcg ggcctgggca cgcgggctgg cgcgcaatcg
     1261 cgatccgcag cgcatcgcct cggtggcgtc gttcttcgtc agccgcgtgg acagcaaggt
     1321 ggacaagcgg ctcgacgcgc tggccacgcc cggggcgcag cgcctcaagg gccgcatcgc
     1381 gatcgccaac gccaggctcg cgtaccggcg cttccgcgag ctgatggccg aggccgacat
     1441 cgcgcgccag ctcgcgcgcg gtgcgcgccc gcagcgcccg ttgtgggcca gcaccagcac
     1501 caagaacccg gcctaccgcg acgtgctcta tgtcgagtcg ctgatcggtc ccgacaccgt
     1561 gaacacggtg ccgcccgaga cgctggacgc cttcgtgtac cacggcgaag cgcgcgccac
     1621 gctggagcag gacgtggagg cggcgcagcg cgacatcgcc gccctggccg agctgggcat
     1681 cgacctcgcc gccgtcacgc gcgagctgga aagcgagggc gtggccgcct tccgcgactc
     1741 ctgggacaag ctgctcgcca gcctgaagga caagtgcttc gcggtggcga aagacttcgc
     1801 gcgctgatca ccggtcgcgc tcagcggccc agaccgtaga gcgcgcgcgc gttgtcgcac
     1861 aggatgcgcg cgcgcgcccg cgccggcacg ccgtggaagt tctcctcgat cgcgcgctgc
     1921 gattccggcc aggtgctgtc cgcgtgcgga tagtcggacg cccacatcac gcgctcttcg
     1981 cccagcaagt cgagcaagcg cagcccggcg tgatcggtct ggaacgtggc ccagacctga
     2041 cgccggaagt agtggctcgg cggcatgcgc agcgccagcc cgccgtgcac gcgccaccac
     2101 tcctcggcgt cgaggaactt gcgatagctg tcgtccatcc gctcgagcag gtaggggagc
     2161 cagccgatcc cggtctcggc gagaacgatg ttgaggccgg ggtaacgttc caaggctccg
     2221 gagaagatga ccgccgcgag cgcctcgtcc atttgcagcg tcgagcacga tacgcgcacc
     2281 cccatgttgc cggcgtcgga tgccttgggc ccgctccaga ccaccgtgcg atgcccgccg
     2341 cccagatgga agccgatcgg gatgccggtc tcggcggccg cttcccacag cggggtccag
     2401 gcctggtccc acagcggcag ctcgacgcgc gcggcgagga acatgccggt gcggatgccg
     2461 agccggcgca agtgccgcac ctcttcggcc gccatgcgcg gatcgtcgat gggcaccagg
     2521 ccggcgccca ccaggcgctc ggacgccgaa gcgcagaatt cggcgagcca ctcgttgtag
     2581 gcccggtagc acacctgacg cagcgccggg tccgcgatca gtagcggcac gatcggcccg
     2641 tacatgaccg tggcgtccac gccgtcgcgg tccatgtcgg ccaggcgcaa cgcagtggtc
     2701 gtgggccgca gtacgcccgg ctcgagcacg ccgccgcgct cgagcgccag ccgccggccc
     2761 cccatggcgc ccgcggcgcc cttgcgcccg ccccaggatt cccaccggtc gtcgccgcac
     2821 acccagtacg gcccgtcggc ggtgtcgacc accttcgggg cgcgctcgcg ccaggcagcc
     2881 gggacgcgct tctgccagag gtccttgggc agccactgca tgtcgatgtg atcgtcagcg
     2941 gagatgatgc ggtagtccat cgggtcctcc tcgcgggtgg tctcagcgca tcggcggcgt
     3001 ccgccccccg acgacgcgtc tgcgcctatg gtagatgccc cgcacgctat aattcaaatc
     3061 tttgccccgg agtcatcggc ccgtgcctga gctgcgtttt cgtctcccgg ggggcgctgc
     3121 gtgctgcagg gggtgaagct cgcatgcgcg cgcggaggcc gcccgctgtt cggcgagctg
     3181 tccttcgatc tcgagccggg cgagctgttc tgggtgaccg gccccaacgg cagcggcaag
     3241 accagcctgc tgcgcatgct ctgcacgctg ctgccgcccg aatcgggcga ggtgcgctgg
     3301 cgcgggcatc cggtgcgcgc gctgggcgag agctatcgcg cggagctcgc ttacctgggg
     3361 cacgcgccag cggtgaagga cgatctgagc gcgctggaga acctgcgctt cgcgctcgcc
     3421 cagcaaggca cggcgcccgc cgagcgcgag ctgctcggcg cactggaggc cttcggcctg
     3481 gcgcaacgcg aggaccttcc gctgcgctcg ctgtcgcagg ggcagaagcg acgcgtggcg
     3541 ctggctcgcc tggcattctg cgcgcagcgc gcgctgtgga tcctggacga gccgctgacc
     3601 gcgctagaca gtgcggcggt ggagctggtg cgcggccacg tcgaggcgca tctcgcgcgc
     3661 ggcgggctgg tggtgctgac cagccatcag gatgtggact tgagccgcat gcgggtacag
     3721 cggctgaggt tggacgcgtg aacccccccg ccggagaaac cagccggatc gcggtcggcc
     3781 gctccgctcc cgccgcttcg cgcgcgccgc tgtggccggt gctcgccggc gtggtgcagc
     3841 gcgatttgct gctggcgatg cgccgccgct cggatgtact gacgacattc tttttcttca
     3901 tcatcgtggt gagcctgttt cccctcggcg tgggccccga gcccggcacg ctgcgcgaga
     3961 tcgcgcccgg cgtcgtgtgg gtggccgcgt tgctcgcgtc catgctggcg ctcacgcggc
     4021 tgttcgcggg ggattacgcc gacggtacgc tggagcagct ggcgctggtg ccgcagccgc
     4081 tggtgctgct ggtgctggcc aagatcgcgg cgcactggct ggtgaccggt ctgccgcttg
     4141 tcgtgatcgc accgctgctc ggcctgcagt tcgatctgcc gcccgacgcg ctggcggtga
     4201 tgacggcggc actggtgctg ggaacgccgg tgttgtcgat gatcggggca gtgggggccg
     4261 cgctcacgct cggtgtgcgc ggcgcgggcg gcctgctagc gctgctggtg ctgccgctgt
     4321 acgtcccggt gctgatcttc ggcgccggcg ccgtgacggc cagcgccgcg ggcctgagcg
     4381 ccgcgggcca cttgtcgttg ctgggcgcgg tgctgctgct gtccctggtg ttcgctccct
     4441 gggccacggc ggcggcgatc cgcatcgcgc tggagtagcg gcatggcggt caatctctgg
     4501 cgtttcgcct cgccgcaagt gttctacccg ctcgccgggc gcatgctgcc gtggttcttc
     4561 ggcgcggccg tcctgttcgc gctggccggg ctgtacgtcg gtctgttcgt cgcccccacc
     4621 gacttccagc agggcgaggc gtatcgcatc atcttcgtgc acgtgcccgc ggcctggatg
     4681 tcgatgttcc tctacctggt gatggcgttc tggtgcgccg tgggcctggc gatgaacacg
     4741 cgcctgtcct tcatgatggc gctggcgctc gcaccgaccg gggcgctgat gaccttcctc
     4801 gcgctgtgga ccggatcgct gtggggcaag ccgacctggg gcacgtggtg ggtgtgggat
     4861 gcgcgcctga cctcggagct gatcctgctg ttcctctaca tcggcttcct gtcgctgcac
     4921 gcggcgatcg acgacgtgcg ccgcgcggac aaggccgccg cgctgctggc gctggtgggc
     4981 gtggtgaacg tgccgatcat ctacttctcc gtgaagtggt ggaacacgct gcaccagggc
     5041 gcttcggtca gcctgacgcg cgcgccgtcg atggccgcga cgatgctgac cggcatgctg
     5101 ctgatggcgc tcgccgcctg gatgtacagc atcgcggcga gcctgatgcg cgtgcgcgcg
     5161 attatcctcg agcgcgaggc cgatgcggac tgggtgcggg ccctggacga agggccggtt
     5221 ccggaatgat ccgttggcag gtggtccgga aatgatttgg cgcagcgcag ccgagttctt
     5281 cgccatgggc gggtatgcgg tatacgtatg gggctcgtac ggcgttgcgg cgtgcctgct
     5341 ggccgcggaa ctgtggctgc tcgcccgccg ccggcgaact ctgctgcgcc gcctgggtcg
     5401 tagcagcaca ccccgagagc agcatcgatg aagccgagac acaagcgcct ggcactgatc
     5461 ggcgcgggca tcgccctgct gggcggggcc gccttcctga tcctgaacgc gttccagagc
     5521 aacctggtgt tcttctacag tccctcgcag gtcgccgcgc acgaggcgcc gcaagggcgg
     5581 gcgttccgca tcggcgggat ggtgcagacc ggcagcgtgc agcgcgaggc cgacggggtt
     5641 accgtgcggt ttacg
//
LOCUS       DASPGE010000198         6799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_171433, whole genome shotgun sequence.
ACCESSION   DASPGE010000198 DASPGE010000000
VERSION     DASPGE010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6799
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171433"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..951)
                     /locus_tag="VD791_06370"
     CDS             complement(196..951)
                     /locus_tag="VD791_06370"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXV07635.1"
                     /translation="MSRREIILQELGLWPVWVPREGAGAAPDGTPDGTRGATHDTAPE
                     ALPEGAPGSRAERIARMDWPALEEAVRACTACPLHARRKQAVFGVGDERADWLFVGEG
                     PGAEEDARGEPFVGQAGRLLDNMLAAIGLRRGRDVYIGNVVKCRPPGNRVPEPNEAAA
                     CEPFLLRQIALIQPRLIVALGKTAASNLLRTDASIGSLRGRLHDFRGTPLLVTYHPAY
                     LLRNLPDKSKAWEDLCLAREVMQRLKAGEPAPI"
     gene            complement(948..1409)
                     /gene="rimI"
                     /locus_tag="VD791_06375"
     CDS             complement(948..1409)
                     /gene="rimI"
                     /locus_tag="VD791_06375"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HXV07636.1"
                     /translation="MSAVMRPHRVLRPMRGSDLDQVLAIERAIYTHPWTAGNFRDSLQ
                     AGYSCWVMERDGAMVGYGVLMIGVAEAHLLNLSVAREWQRRGLGRELLQHFVQLARQL
                     EAERLFLEVRPSNASARGLYARAGFRELYVRRGYYPADGGREDAILMGIDL"
     gene            complement(1406..2110)
                     /gene="tsaB"
                     /locus_tag="VD791_06380"
     CDS             complement(1406..2110)
                     /gene="tsaB"
                     /locus_tag="VD791_06380"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HXV07637.1"
                     /translation="MNILALDTSGEWCSAALWLDGRLASRGEHAGQHHSALILPMIDA
                     LLRESGLALTDLDGLAFGAGPGSFTGLRIACGVAQGLAFGAGLPVVPVGTLEALAEAS
                     AAPRVVAALDARMDEVYHAAYVREDSGWREVHGPSLCAPAAAPPVEGGDWTGCGNGFA
                     VQRGALRVRYAGQLRDVRDDLQPHAREMARIAASRLAQGGGLDPAQAAPLYVRDKVAL
                     SVAERAARQALAPERP"
     gene            2364..3647
                     /locus_tag="VD791_06385"
     CDS             2364..3647
                     /locus_tag="VD791_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV07638.1"
                     /translation="MLHPLRRLGAIATVVTAALAAAPSPAQEPLRIGVITFLSGPAAG
                     PFGVPAKNASDLLAELLNKGGMIPGYEKKGFGGRPIELVYVDEAGGPTKVVTEYRNLV
                     ERQNVDLVIGVISSGDCLAVAPVAEEMKKLTVLFDCATNRIFEEATYKYVFRTTTMAT
                     QENVAAARYISENFPGLKTFSGIDPNYAWGQDAWSDFTEALKILKPQAQIGTVVWPKL
                     GAGQYGTEISAVLGSGAEVVHNGLWGGDLEAYVMQAQPRGLLAKMPNIIITGETIIDR
                     MSAQLPDGTILGARGPNGPFAPPTTLNKWFAKAYRDKFNIGAVYASYHMATAFFGAKA
                     AYEKAQAAAKGKDPSIDEIIKAFEYLKFESPAGTVDMSLGKGHQAVQHVPYGTVKKVD
                     GKITLVNIKYYPPEQISPPDGMKSLDWIRSGFKRK"
     gene            3660..4916
                     /locus_tag="VD791_06390"
     CDS             3660..4916
                     /locus_tag="VD791_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV07639.1"
                     /translation="MSHDADASRRKALKQTAALSAAAALPLGFPAIVRAQPEVIRLGH
                     LTPRTGFLGQAGEQGFRGATLAVEEANAAGGALGRRLELIAEDSVNPATAVNKAQKLI
                     ERDKVACLLGEVSSASALAIGEQALRHRTPFFNTGSNSDALRGQNCNRYMFHIEGSNT
                     MYTKTIGTWQKDNNLIKGARWYFLTADYAFGHDLYRVSSRFLQENGGVVLANDMVPTN
                     TADYSAYILKIRSLKPDYIYLNLAGVDQTTFLKQYREYSLPFPLTGGVMDTVGFWAAG
                     IDNISGVWQSLWYHGLKNAAAQAFTKRFFDRFKLPPENQAWGDYVGVRIVLQALAETG
                     RTDAASIILYLEKGATFDLLKDRKGMFRGWDHQLLQEMYVVKVKDKAQAKDRWDIFDV
                     VEAVPGAKESLEVIQPTPAENPCRMA"
     gene            4921..5805
                     /locus_tag="VD791_06395"
     CDS             4921..5805
                     /locus_tag="VD791_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV07640.1"
                     /translation="MTPLVPELVLEQVINGLLVGSFYIILSLGLSLIFSLGGVVNLAH
                     GAFYAVGAYLAYEVQRHGGFLGATVVSPVAVALIGILIERLLLSRLYGKDPALGLLFT
                     FGLALTAEQSLRMIWGTTGLPFSIPDALRGQLIVGDFIYSYYRLTILAAAAAAVVGCW
                     LLLNKTAFGLVVRAGVRDAEMVRALGISLNPALAAIFALGVGLAALAGVMSAPLAGVQ
                     PAMGNEILTATFVVVVIGGLGSFWGVVLAGLLVGVVRGLTVHFYPPAAEASMYLLMVL
                     VLLFRPRGLMGERFEKFE"
     gene            5802..>6799
                     /locus_tag="VD791_06400"
     CDS             5802..>6799
                     /locus_tag="VD791_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07641.1"
                     /translation="MTRHSERPRQNRRRNREDAARWAAELAARYTAASTPDARAAVLG
                     ALDLPYTLDEDTALALYAIQPALSSEFIQRHLPRGRRADDARLPWNRLMGQALARADD
                     PLHFALYRMQAPPEEWARDSAELARRVSDAQALCEELERRHPQRWRPDVGLRLHALAL
                     ERGQHMLPYLERHAAEVWSPRRRSGYEEMLELAQRRGWWQLWATLLAACAPPSQYDRE
                     VMALVSGAQTAEAELRQRLLVLAGVWAPLHAGAPPKRAGRALKEDTLLALYERFPHLA
                     RGPFRTQLEPTANRPLSRLIDLALERRDAELVDHFAARLAARLERSGAERLMQAVVR"
BASE COUNT         1025 a         2458 c         2309 g         1007 t
ORIGIN      
        1 ccacgccgcc ttgacctgct cgtcctcggc ctggatcgtg ttgtagccgc agccgcccag
       61 gccgagcgtc gcaaccgcgg caagtaccca actcagcagc ctgttcttca tgtcggtggt
      121 ctcctgtcga gccggaataa gtagtttaag tcgcgcctta agggcgcatt cggcggtgtg
      181 cagcatccgc ggccgtcata tcggggccgg ttcccctgcc ttcaagcgct gcatcacctc
      241 gcgcgccagg cacaggtcct cccaggcctt ggacttgtcc ggcagattgc gcagcaggta
      301 tgccggatga taggtcacca gcagcggggt gccgcgaaaa tcgtgcagcc ggccgcgcag
      361 gctgccgatc gaagcgtccg ttcgcagcag gttcgacgcc gccgtcttgc cgagcgccac
      421 gatcaggcgg ggctggatga gggcgatctg acgcagcagg aacggctcgc acgccgccgc
      481 ttcgttcggc tccggcacgc ggttgccggg agggcggcat ttgaccacat tgccgatgta
      541 cacgtcgcgg ccgcgccgca atccaatcgc cgccagcatg ttgtccagca ggcggccggc
      601 ctggcccacg aacggttctc cgcgcgcatc ttcctcggcg cccggccctt cacccacgaa
      661 cagccagtcg gcgcgctcgt cgccgacgcc gaacaccgct tgcttgcgcc tggcatgcag
      721 cgggcaggcg gtgcaggcgc gcaccgcttc ctcgagcgcc ggccagtcca tgcgtgcgat
      781 gcgctcggcg cgcgagccgg gcgcgccctc aggcagcgct tcgggcgcgg tgtcgtgtgt
      841 cgcgccgcgc gtcccatcgg gcgtcccatc gggcgccgcg ccggcgccct cgcgcggcac
      901 ccaaacgggc caaagcccca gctcctgcag gatgatctcg cggcggctca cagatcgatc
      961 cccatcagga tcgcgtcctc gcggccgccg tcggccggat agtagccgcg ccgcacgtac
     1021 agctcgcgga agccggcgcg cgcgtacagg ccgcgcgcgg aagcgttgga cgggcgcact
     1081 tccaggaaca ggcgctcggc ctcgagctgg cgcgcgagct ggacgaagtg ctgcagcaat
     1141 tctcggccca gcccccggcg ctgccattcg cgggcgacgc tcaggttcaa caagtgcgcc
     1201 tcggccacgc cgatcatcag cacgccgtag cccaccatcg ccccgtcgcg ctccatcacc
     1261 cagcagctgt accccgcctg cagcgaatcg cgaaagttgc ccgccgtcca gggatgcgtg
     1321 tagatggcgc gctcgatcgc gagcacctgg tcgagatccg agccgcgcat cggccgcagc
     1381 acgcgatgcg ggcgcatcac cgcgctcatg gccgctccgg cgcgagcgcc tgccgggccg
     1441 cgcgctcggc cacgctcagc gccaccttgt cgcgcacgta caggggcgcg gcttgcgcgg
     1501 ggtccagccc gcctccttgc gcgaggcggc tcgcggcaat gcgcgccatc tcgcgcgcgt
     1561 gcggctgcag atcgtcgcgc acatcgcgca gttgccctgc gtagcgcacg cgcagagcgc
     1621 cgcgctgcac cgcgaagccg ttgccgcaac cggtccagtc gccgccctcg accggcggtg
     1681 ccgccgccgg tgcgcacagg ctgggtccat gcacttcgcg ccaccctgag tcttcgcgca
     1741 cgtaggccgc gtgatagacc tcgtccatgc gcgcgtcgag cgccgccacc acgcgcggcg
     1801 cggcgctcgc ctccgccagc gcctccagcg tgccgaccgg caccaccggc agcccggcgc
     1861 cgaaggcgag cccttgcgcg acgccgcacg cgatgcgcaa cccggtgaac gagccgggcc
     1921 cggcgccgaa cgcgagcccg tcgagatcgg tcagggccag ccccgactcg cgcagcagcg
     1981 cgtcgatcat gggcaggatg agcgccgagt ggtgctggcc ggcgtgctcg ccgcggctcg
     2041 cgagccggcc gtcgagccag agcgcagccg agcaccattc gccggatgta tcgagcgcga
     2101 gaatgttcaa gatgaggcgg ccgcaccgct ttcccgccgc gccgctttgt ctccgcatcg
     2161 gcggcgcggg gcgaccgcag gcgcggtgca ggctcgcggc tattctagcg agtttcgttg
     2221 ctataatccg cacggttggg cgggaaaacc gggcgccgtg tgcacggagc gcccccgcgt
     2281 cgagaacaac gagccgcgcg tcccagcgag cggcacgccg tgcgcttttc gccgttcgcc
     2341 gtcgaaacct cgggagacag cccatgcttc atccgctgcg ccgccttggc gcgatagcca
     2401 ctgtggtcac cgccgcgctc gccgccgcgc cctcgcccgc ccaggagccg ctgcgcatcg
     2461 gcgtgatcac gttcctgtcg gggcccgccg ccggcccctt cggcgtgccg gccaagaacg
     2521 cctccgatct gctggccgag ctgctcaaca agggcggcat gatcccgggc tacgagaaga
     2581 agggcttcgg cggacgcccg atcgagctgg tgtacgtgga cgaggccggc ggcccgacca
     2641 aggtggtcac cgagtaccgc aacctggtcg agcggcaaaa cgtggacctg gtgatcggcg
     2701 tgatctcctc cggggactgc ctggcggtgg cgccggtggc cgaggagatg aagaagctca
     2761 ccgtcctgtt cgattgcgcc accaaccgca tcttcgagga agccacctac aagtacgtgt
     2821 ttcgcaccac caccatggcc actcaggaga acgtggcggc ggcgcgctac atctcggaga
     2881 acttccccgg cctgaagacc ttctcgggca tcgatcccaa ctacgcctgg gggcaggacg
     2941 cctggtccga cttcaccgag gcgctgaaga tcctcaagcc gcaggcgcag atcggcaccg
     3001 tggtgtggcc caagctgggc gcgggccagt acggcaccga gatctcggcg gtgctcggca
     3061 gcggcgccga ggtggtgcac aacggcctgt ggggcggcga cctcgaggcc tacgtcatgc
     3121 aggcccagcc gcgcggcctg ctggcgaaga tgcccaacat catcatcacc ggcgagacca
     3181 tcatcgaccg catgagcgcg cagcttcccg acggcaccat cctcggcgcg cgcggcccga
     3241 acggtccgtt cgcgccgccc accacgctga acaagtggtt cgccaaggcc tatcgcgata
     3301 agttcaacat cggcgcggtg tacgcctcct atcacatggc caccgccttc ttcggcgcca
     3361 aggccgctta cgagaaggcg caggccgccg ccaagggcaa ggacccgagc atcgacgaga
     3421 tcatcaaggc gttcgagtac ctcaagttcg aatcgccggc cggtaccgtg gacatgtcac
     3481 tcggcaaggg ccaccaggcg gtgcagcacg tgccctacgg cacggtgaag aaggtcgacg
     3541 gcaagatcac gctggtcaac atcaagtact acccgcccga gcagatcagc ccgcccgacg
     3601 gcatgaagag cctggactgg atccgctccg ggttcaagcg caagtgaagg ggaggcgata
     3661 tgagccacga cgccgacgcg tcgcgccgca aggcgctcaa gcagaccgcg gccttgagcg
     3721 ccgcggcggc gctgccgctt ggatttcccg ccatcgtgcg cgcgcagccc gaggtgatcc
     3781 gcctgggcca tctgaccccg cgcaccggct tcctcggcca ggcgggcgag cagggtttcc
     3841 gcggcgccac gctggcggtg gaggaagcca acgccgccgg cggcgcgctc ggccgcaggc
     3901 tggagctgat cgcggaggac agcgtcaacc cggctaccgc ggtgaacaag gcgcagaagc
     3961 tgatcgagcg cgacaaggtc gcctgcctcc tgggcgaggt gagctcggct tccgcgctgg
     4021 cgatcggcga gcaggcgctg cgccacagga cgccgttctt caacaccggt tcgaactccg
     4081 acgcgctgcg cgggcagaac tgcaaccgct acatgttcca catcgaaggc agcaacacca
     4141 tgtacaccaa gaccatcggt acatggcaga aggacaacaa cctgatcaag ggcgcgcgct
     4201 ggtatttcct caccgccgac tacgccttcg gccacgatct gtaccgggtg tcgtcgcgct
     4261 tcctgcagga gaacggcggc gtggtgctgg ccaacgacat ggtgcccacc aacaccgccg
     4321 actacagcgc ctacatcctc aagatccgct cgctcaagcc cgactacatc tacctcaacc
     4381 tcgccggcgt ggaccagacc accttcctca agcagtaccg cgagtacagc ctgcccttcc
     4441 cgctcaccgg cggcgtgatg gacacggtcg gcttctgggc ggcgggcatc gacaacatct
     4501 ccggcgtgtg gcagagcctg tggtaccacg gcctcaagaa cgccgccgcg caggcgttca
     4561 ccaagcgctt cttcgaccgc ttcaagctgc ctccggagaa ccaggcctgg ggcgactacg
     4621 tgggcgtgcg catcgtgctg caggcgctgg cggaaaccgg gcgcacggac gccgcctcca
     4681 tcatcctgta tctggagaag ggcgccacct tcgatctgct gaaggaccgc aagggcatgt
     4741 tccgcggctg ggaccaccag ctcctgcagg agatgtacgt cgtcaaggtg aaagacaaag
     4801 cgcaggccaa ggacaggtgg gacatcttcg acgtggtgga agcggtgccc ggcgccaagg
     4861 agtcgctcga ggtgatccag cccacgccgg cggagaatcc gtgccgcatg gcgtgactgc
     4921 gtgacacccc tcgtccccga gctggtcctc gagcaggtca tcaacggcct gctggtcggc
     4981 tccttctaca tcatcctgtc gctcgggctg tcgctgatct tcagcctcgg cggcgtggtg
     5041 aatctggcgc acggggcctt ctatgccgtg ggcgcgtatc tcgcctacga ggtgcagcgg
     5101 cacggcggct tcctcggcgc gacggtcgtc tcgccggtgg cggtggccct gatcggcatc
     5161 ctgatcgagc gcctgctgct gagccgcttg tacggcaagg atcccgctct gggtctgctg
     5221 ttcaccttcg ggctggcgct caccgccgag cagagcctgc gcatgatctg gggcaccacg
     5281 gggctgccct tctccatccc ggatgcgctg cgcggccagc tcatcgtcgg cgacttcatc
     5341 tacagctact accggctcac catcctggcg gcggccgccg cggcggtggt cggctgctgg
     5401 ctgctgctca acaagaccgc tttcggcctg gtggtgcgcg ccggcgtgcg cgacgcggaa
     5461 atggtgcgcg ccctgggcat cagtctgaat ccggcgctgg ccgcgatctt cgcgctcggc
     5521 gtcgggctgg cggcgctggc tggggtgatg tcggccccgc tcgccggcgt gcagccggcg
     5581 atgggcaacg agatcctcac cgccaccttc gtcgtggtgg tgatcggcgg tctcggctcg
     5641 ttctggggcg tcgtgctcgc gggcctgctg gtgggcgtgg tgcgcggcct caccgtccac
     5701 ttctacccgc cggccgccga ggcgtcgatg tacctgctga tggtgctggt gctgctgttc
     5761 cggccgcgcg gcctgatggg cgagcgcttc gagaagttcg aatgaccagg cactccgagc
     5821 gcccgcgtca gaaccgccgt cgcaaccgcg aagacgccgc gcgctgggcc gccgagctcg
     5881 ccgcccgcta cacggcggcg agcacgcccg atgcgcgcgc cgcggtgctg ggcgcgctcg
     5941 acctgccgta cacgctggac gaggacacgg cgctggcgct ttacgcgatc cagccggccc
     6001 tgtcctccga gttcatccag cggcacctgc cgcgcgggcg ccgcgccgac gacgcgcgcc
     6061 tgccgtggaa ccggctgatg ggccaggcgc tggcgcgcgc cgacgacccg ctgcacttcg
     6121 cgctgtatcg catgcaggcc ccgcccgagg aatgggcgcg cgacagcgcc gagctcgcgc
     6181 gccgcgtgtc cgacgcgcag gccttgtgcg aggagctcga acgccgtcac ccgcagcgct
     6241 ggcgcccgga cgtcggcctg cgcctgcatg cgctggcgct ggagcgcggc cagcacatgc
     6301 tgccttatct ggagcggcac gccgccgagg tctggtcccc gcgccggcgc agcggttacg
     6361 aggagatgct cgagctggcg cagcggcgcg gctggtggca gctctgggcc acgctgctcg
     6421 ccgcctgcgc gccaccgtcc cagtacgatc gcgaggtgat ggccctggtg agcggcgccc
     6481 agaccgccga ggcggagctg cgccagcgcc tgctggtgct cgccggcgtg tgggcgcccc
     6541 tgcatgcggg cgcgccgccc aagcgcgccg ggcgcgcgct gaaagaagac acgctgctgg
     6601 cgctctacga gcgcttcccg cacctggcgc gcggaccgtt ccgcacgcaa ctcgagccca
     6661 ccgcgaaccg gccgctgtcg cggctgatcg atctggctct cgagcggcgc gacgcggagc
     6721 tggtggacca cttcgccgcc cgcctcgctg cgcggctgga gcgctcgggc gccgagcggc
     6781 tgatgcaggc cgtggtgcg
//
LOCUS       DASPGE010000199         4536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1459150, whole genome shotgun sequence.
ACCESSION   DASPGE010000199 DASPGE010000000
VERSION     DASPGE010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459150"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VD791_06405"
     CDS             complement(<1..783)
                     /locus_tag="VD791_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV07642.1"
                     /translation="MNALRLVAPVLVGLVALLLWQGLVTWYEVPVYLVPSPLRVVQTL
                     MRDWGLLTGSLLVTLRIALLALLLALVLGIAVAFLFVQSRTVEASLFPYAILLQVTPV
                     VAIAPLIIIWVKDPTLALVICATIVALFPIISNTAQGLRSVDPELLDLFRLNRATRLQ
                     TLLRLRIPSALPYFFAGLKVSSGLALIGAVVAEFVAGTGGTASGLAYQILQAGLQLNI
                     PRLFAALLLITLAGEQQLHHVDLRSAHFHVDVQAVLAVSALGH"
     gene            complement(780..1514)
                     /locus_tag="VD791_06410"
     CDS             complement(780..1514)
                     /locus_tag="VD791_06410"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV07643.1"
                     /translation="MHLERVEKVFPNGVRALERIDLDIREGEFVTLLGPSGCGKSTLL
                     RLIAGLLAPSAGAITQAGDGGAPRRLAFVFQHPTLMPWASVSRNVRLPLDLAGVAHGE
                     ADGRVRAALKLVGLGEFARVYPRELSGGMQMRASIARALVTEPRLLLMDEPFGALDEI
                     TRNRLDRDIRELWARQRLTTVFVTHSIYEAVFLSTRVAVMSPRPGRILHQVAIEEPHP
                     RTEAFRVSQRFAAHCAQLSALLAGPG"
     gene            complement(1554..2528)
                     /locus_tag="VD791_06415"
     CDS             complement(1554..2528)
                     /locus_tag="VD791_06415"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HXV07644.1"
                     /translation="MERRVIGSSFDTVFEYDYSVQAEDMRGLYEKAKRDQWNASRDIA
                     WDTPQTDDGRVLADELIDIHGSPIWERLGQAERVEINRRIAAWRLSVLLYGEQGAMLA
                     CSQLVNIVAGTDQKFFQATQVADEARHNEVLERYLECRLGGLHYPMPENERFLFDAIL
                     TDPRWYIKTIALQLVAETFAVSMFRMMGQAAADPVLREICGRILQDESRHMGFGMLSL
                     PAVVRDASAAERGEMEDFTCLALEKVLTGFFPLEAYRDLGFSAAQIDEVRSYRRDAAA
                     RNDYAVYRKYFRRDMHASMVANLVRIGLLTDRVRPRLAALGIALSPAA"
     gene            complement(2554..3381)
                     /locus_tag="VD791_06420"
     CDS             complement(2554..3381)
                     /locus_tag="VD791_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXV07645.1"
                     /translation="MDLQTVLLAKADRVATLTLNRPERLNAMNRTMLAEINRACDAVA
                     ADDDVRVLVVTGAGAAFSSGFDLKEQMEAQPAGAADWRRILDQDFGAIMRFWSLSKPT
                     IAAVQGHCLAGACELAMACDITIAAENAVFGEPELKFGAGIVAMLLPWLTGPKQAKEI
                     IFNGIDNLSAREALRLGLINRVVPDGAHLNAALAMARRIAAIDPELMRQTKKAINRTY
                     EIQGMGEALATALDVDLDIESRGSPDKRRFMEIARRDGLRAALAWRDARFAVDPSEQ"
     gene            complement(3396..4535)
                     /locus_tag="VD791_06425"
     CDS             complement(3396..4535)
                     /locus_tag="VD791_06425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HXV07646.1"
                     /translation="MTVACIEDLRRIARRRLPRVIFDFVDGGAMDEVTLRANRSDFQR
                     VALAPRVLTDVSRRDASTTVLGQDIALPLIIAPTGLAGLLWRKGELALARAAAQAGIA
                     YCMSQMAACSIEEVAQASTLPFWFQTYLFKDRGINEGLIQRAARVGCRVLVVTVDTKV
                     QGPRDRDVRNGFTVPPRLTPASVLDMARRLRWLRGVALGPRVTFANLAGTVLDGDDII
                     NIARFATQQYDYTLAWSDIDWCRARWRGKLAIKGILTPEDARLAVEHGADALIVSNHG
                     GRQLDGAPSPLAVLPAVVDAVQGRAEVILDGGVRRGGDILKALALGARACMAGRPFLY
                     GLAAAGERGVAQAIGLLAGEIDNGLALLGRPSARALDRTALRWPR"
BASE COUNT          718 a         1587 c         1592 g          639 t
ORIGIN      
        1 gtggccgagg gcacttaccg caagcacggc ctggacgtcg acgtgaaaat gggcggaccg
       61 caggtcaacg tggtgcagct gctgctcgcc ggccagggtg atcagcagca gcgcggcgaa
      121 cagccgcggg atgttgagct gcagcccggc ctgcaggatc tggtaggcga gcccgctggc
      181 ggtgccgccc gtgcctgcga cgaactccgc cacgaccgca ccgatcagcg ccagcccgct
      241 ggagaccttc agcccggcga agaagtaggg cagggcgctg gggatgcgca ggcgcagcag
      301 cgtctgcagc cgcgtggcgc ggttcaaccg gaaaaggtcc agcagttcgg ggtcgacgct
      361 gcgcagcccc tgggcggtat tggagatgat cgggaacaga gcgacgatgg tggcgcagat
      421 caccagcgcc agggtcggat ccttcaccca gatgatgatc agcggggcga tggccaccac
      481 cggcgtcacc tgcagcagga tggcgtaggg gaacaggctg gcttcgacgg tgcggctctg
      541 cacgaacagg aacgccaccg cgatgcccag caccagcgca agcagcagcg ccagcaacgc
      601 gatgcgcaac gtcaccagca gcgatccggt gagcagcccc cagtcgcgca tcagcgtctg
      661 caccacccgc agcggcgagg gcaccaggta gaccggcacc tcataccacg tgaccaggcc
      721 ttgccacagc agcagcgcga ccagacccac gagcaccggg gcgaccaacc tgagtgcgtt
      781 catcccggac ctgccagcag cgcgctcaac tgcgcgcagt gcgcggcgaa gcgctgcgac
      841 acgcggaacg cctcggtgcg cggatgcggc tcctcgatcg ccacctggtg cagaatgcgt
      901 cccgggcgcg gcgacatcac cgccacgcgg gtggacagga acaccgcctc gtagatgctg
      961 tgcgtcacga acaccgtcgt gagccgctgc cgcgcccaca gctcgcggat gtcgcggtcg
     1021 aggcggttgc gggtgatctc gtccagcgca ccgaacggct cgtccatcag cagcagccgc
     1081 ggctcggtga ccagggcgcg ggcgatggag gcgcgcatct gcatgccgcc cgagagctcg
     1141 cgcggataca cgcgcgcgaa ttcgcccagg ccgaccaact ttagcgcggc gcgcacccgg
     1201 ccgtcggcct cgccgtgcgc gacgcctgcc agatccagcg gcagacgcac attgcgcgac
     1261 accgatgccc agggcatcag agtcggatgc tggaacacga aggccagccg gcgcggcgcg
     1321 ccgccgtcgc ccgcctgcgt gatggcgccg gcgctgggcg ccagcagccc ggcgatcagg
     1381 cgcagcagcg tgctcttgcc gcagcccgac ggcccgagca gcgtgacgaa ctcgccctcg
     1441 cggatgtcga ggtcgatgcg ctcgagcgcg cgcacgccgt tggggaagac cttctcgacc
     1501 cgctcgaggt gcacccgctg gccttgccga gaggtcgctg cgaccgcgcg gcgtcaggcc
     1561 gccggggaga gcgcgatgcc cagcgcggcc agccgcgggc ggacgcgatc ggtgagcagg
     1621 ccgatgcgca cgaggttggc caccatcgac gcgtgcatgt cgcgccggaa gtacttgcgg
     1681 tacaccgcat agtcgttgcg ggcggcggcg tcgcggcggt aactgcggac ctcgtcgatc
     1741 tgggcggcgg agaaccccag gtcacggtag gcctcgagcg gaaagaagcc ggtcagcacc
     1801 ttctccagcg ccaggcaggt gaagtcctcc atctcgccgc gttccgcggc gctggcgtcg
     1861 cgcaccaccg ccggcagcga cagcatgccg aagcccatgt gccgcgactc gtcctggagg
     1921 atgcgcccgc agatctctcg gagaaccggg tcggcggcgg cctggcccat catcctgaac
     1981 atggagacgg cgaacgtctc ggccaccagc tgcagcgcga tcgtcttgat gtaccagcgc
     2041 ggatcggtga ggatcgcgtc gaacaggaag cgctcgttct ccggcatcgg atagtgcagc
     2101 ccgcccagcc ggcactcgag gtagcgctcc agcacctcgt tgtggcgcgc ctcgtcggcg
     2161 acctgggtgg cctggaagaa cttctgatcg gtgccggcca cgatgttcac cagctggctg
     2221 caggcgagca tggccccctg ctcgccgtag aggagcaccg acaggcgcca ggcggcgatg
     2281 cgccggttga tctcgacgcg ctcggcctgc cccagccgct cccagatggg actgccgtgg
     2341 atgtcgatca gctcgtcggc caggacgcgg ccgtcgtcgg tctgcggagt gtcccaggcg
     2401 atgtctcggg aggcgttcca ctggtcgcgc ttggccttct cgtagaggcc gcgcatgtct
     2461 tcggcctgca ccgagtagtc gtactcgaag accgtgtcga agctgctgcc gatcacgcgc
     2521 cgttccatgc ctgcctctcg gaagatgggg agctcactgc tcggaagggt cgacggcgaa
     2581 tcgcgcgtcg cgccatgcga gcgcggcgcg caatccgtcc cggcgggcga tttccatgaa
     2641 ccgccgcttg tccggggaac cgcgcgattc gatgtccagg tcgacatcga gcgcggtcgc
     2701 gagcgcctcg cccatgccct ggatctcgta ggtacggttg atcgctttct tggtctggcg
     2761 catcagctcg ggatcgatcg ccgcgatgcg ccgcgccatc gccagtgccg cattgagatg
     2821 cgcgccgtcg ggcacgacgc gattgatgag gcccaggcgc agcgcctcgc gcgcgctcag
     2881 attgtcgatg ccgttgaaga tgatctcctt cgcctgcttc gggccggtga gccacggcag
     2941 gagcatggcg acgatcccgg cgccgaactt gagctccggc tcgccgaaca cggcgttctc
     3001 cgcagcgatc gtgatgtcgc acgccatggc gagctcgcac gcgcccgcaa ggcagtgccc
     3061 ctgcaccgct gcgatcgtcg gcttggagag cgaccagaag cgcatgatcg cgccgaaatc
     3121 ctggtcgagg atcctgcgcc agtcggctgc gcccgccggc tgtgcttcca tctgctcctt
     3181 gagatcgaag cccgacgaga atgccgcgcc cgcgccggtg acgaccagca cgcgcacgtc
     3241 gtcgtcggct gccacggcgt cgcacgcccg gttgatctcc gccagcatcg tacggttcat
     3301 cgcgttcagc cgctccggcc ggttgagcgt cagcgtcgcg acgcgatccg ccttggcgag
     3361 aagaacggtt tgcaggtcca cgatcgatcc tccgctcacc gcggccagcg cagtgcggtg
     3421 cggtccaacg ccctcgcgct cggccgaccg agcagcgcca ggccgttgtc gatctcgcca
     3481 gcgagcaggc cgatcgcctg cgccacgccg cgctccccgg cggcggccag gccgtagaga
     3541 aaagggcggc cggccatgca cgcgcgggcg cccagcgcga gcgccttgag gatgtcgccg
     3601 ccacggcgca cgccgccgtc gagaatgact tccgcgcgac cctgcaccgc atccaccacc
     3661 gcgggcaaca ccgcgagcgg cgagggcgcc ccgtccagct ggcgcccgcc gtggttggag
     3721 acgatcagcg cgtccgcgcc gtgctcgacc gccaggcgtg cgtcctcggg cgtgaggatg
     3781 cccttgatcg cgagcttgcc gcgccagcgc gcgcggcacc aatcgatgtc gctccaggcg
     3841 agggtgtagt cgtattgctg cgtcgcgaag cgcgcgatgt tgatgatgtc gtcgccgtcc
     3901 agcacggtgc cggcgaggtt ggcgaaagtc acgcgcgggc ccagcgccac cccgcgcagc
     3961 cagcgcaggc ggcgggccat gtccagcacg cttgccggcg taaggcgcgg cggcacggtg
     4021 aagccgttgc gcacgtcgcg gtcgcgcggg ccctgcacct tggtgtccac ggtgaccacc
     4081 agcacccggc agccgacgcg cgccgcgcgc tggatcagcc cttcgttgat accgcggtcc
     4141 ttgaacaggt aggtctggaa ccagaacggc agcgtgctgg cctgcgcgac ttcctcgatg
     4201 ctgcaggcag ccatctggct catgcagtag gcaatgccgg cctgcgcggc ggcgcgcgcc
     4261 agcgccaact cgcccttgcg ccacagcagc ccggccagtc cggtgggcgc gatgatcagg
     4321 ggcagggcga tgtcctgccc gagcacggtg gtggaggcgt cgcgccgcga gacgtcggtc
     4381 agcacccgtg gcgcgagcgc cacgcgctgg aagtcgctgc ggttggcgcg cagcgtcacc
     4441 tcgtccatgg cgccgccgtc gacgaagtcg aagatgaccc ggggcagccg gcgccgggcg
     4501 atgcggcgca gatcttcgat gcaggcgacg gtcatg
//
LOCUS       DASPGE010000200         9107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_406635, whole genome shotgun sequence.
ACCESSION   DASPGE010000200 DASPGE010000000
VERSION     DASPGE010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9107
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406635"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VD791_06430"
     CDS             complement(<1..951)
                     /locus_tag="VD791_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HXV07647.1"
                     /translation="MTRNPYRTLQSFDIAPGKTGKLYSLPALEKAGVGRISRLPVSIR
                     IVLESVLRNCDGGKVTEAHVKQLANWRPAAARVDEIPFVVARIVLQDFTGVPLLADLA
                     AMRSVADKMYKDPKSIEPMVPVDLVVDHSVQVDHYGSAKALDLNMKLEFERNRERYQF
                     MKWGMQAFDTFKVVPPGIGIVHQVNLEHLARGVHRKDGVYYPDTLVGTDSHTTMINGI
                     GVLGWGVGGIEAEAGMLGQPVYFLTPDVVGVHLKGRLRGGVTATDLVLTVTEVLRKAK
                     VVGKFVEFFGEGVENLAVPDRATIANMAPEYGATMGFFPVD"
     gene            complement(963..2405)
                     /locus_tag="VD791_06435"
     CDS             complement(963..2405)
                     /locus_tag="VD791_06435"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HXV07648.1"
                     /translation="MSAPVSNVRPEPDPPLVDIADYVLHHDIKSQEAYDTARLCLMDT
                     LGCGLEALSYPACTKLLGPIVPGTVVPNGARVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILAVADWLSRNALAAGKRPLAIRDVLTAMIKAHEIQGVLAL
                     ENSFNRVGLDHVVLVKVASTAVVARMLGATRDEIINAVSQAWVDGQSLRTYRHAPNTG
                     SRKSWAAGDATSRAVRLALITIKGEMGYPSVLSAKTWGFYDVLFKGNAFRLQRPYGSY
                     VMEHVLFKISFPAEFHAQTAVECAMQLHAQVRDRLDQIQKIVIRTHESAIRIIDKKGP
                     LHNPADRDHCIQYMVAVPLIHGRLTAADYEDDVAADPRIDALREKMECVEDKRFSKDY
                     LDPDQRSIANGITIHFKDGAKTDEVVVEYPIGHRRRRAEGIPLLEAKFRTNLLRRLPP
                     KQAAIILALCKDQQRLEDTPVNEFVDLFVI"
     gene            complement(2515..3678)
                     /gene="prpC"
                     /locus_tag="VD791_06440"
     CDS             complement(2515..3678)
                     /gene="prpC"
                     /locus_tag="VD791_06440"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HXV07649.1"
                     /translation="MTSQAAPAATAPKPKKSVALSGVPAGNTGLCTVGRQGNDLHYRG
                     YDILEFAERAQFEEVAYLLVHGRLPTLAELAAYKQKLRSLRGLPAAVQDILECIPANT
                     HPMDVLRTGCSALGAVLPENVDHKIAEARNVADRLMASFGSMLLYWYHFSHSGRRIEV
                     ETDDDSIGGHFLHLLHGKPPPELWVRAMHTSLNLYAEHEFNASTFAARVIAGTGSDLY
                     SAIAGAIGALKGPKHGGANEFAFDVIGRYDTPDEAEADIVRRVADKQIIIGFGHPVYT
                     ISDPRNQVIKEVARRLSQDAGDMKLFDISARVEETMWREKRMFPNLDWFSATSYHMMG
                     IPTPMFTPIFVISRTSGWSAHVIEQRLDGKIIRPSANYVGPEDRKWVPIEKRK"
     gene            complement(3675..4556)
                     /gene="prpB"
                     /locus_tag="VD791_06445"
     CDS             complement(3675..4556)
                     /gene="prpB"
                     /locus_tag="VD791_06445"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HXV07650.1"
                     /translation="MTSAGAKFRAAVKAETPLQVIGTINAYHARLAERVGYRAIYLSG
                     GGVAAGSLGLPDLGISTLDDVLTDVRRITDVCGVPLLVDADTGFGGAFNIARTVRSLI
                     KSGAGAMHIEDQVQAKRCGHRPGKEIVPREEMVDRIKAAADARTDAQFVIMARTDALA
                     VEGLQAAVDRACACVEAGADMIFPEAVTDLGMYRRFADAVQVPVLANITEFGKTPLFT
                     TDELRSAGVGLVLYPLSAFRAMNQAALNVYRTVRREGTQKSVLDSMQTRDELYDFLDY
                     HAYEDKLDRLFAKEKHK"
     gene            complement(4561..5550)
                     /locus_tag="VD791_06450"
     CDS             complement(4561..5550)
                     /locus_tag="VD791_06450"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HXV07651.1"
                     /translation="MRAPVRVAVTGAAGQIGYSLLFRIAAGEMLGAEQPVLLQLLEIP
                     DDRAQKALRGVMMELDDCAFPLLAGMSASADADAAFRDADIALLVGARPRGPGMERKD
                     LLLENARIFIEQGRALDQAASRDVKVLVVGNPANTNAYIAMSQAPGLDPGNFTAMMRL
                     DHNRAVSQAAAKVGRPVAGLRKVTVWGNHSATQYPDLSQAEVDGQKLWPLIADQAWLE
                     GSFIPTVQKRGAAIIDARGASSAASAANAAMDHMRDWIRGTRPGDWVTMGIPSDGSYG
                     IPQGVIYGYPVTCTGGRYRIVQDIEVSPFSRARMDATHKELLEERDAVASLLK"
     gene            5813..6547
                     /locus_tag="VD791_06455"
     CDS             5813..6547
                     /locus_tag="VD791_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXV07652.1"
                     /translation="MKPAAETPAFQPLYRQIKTLITRSLVSGEWRPGESIPSEVELAG
                     RYSVSQGTVRKAVGELAEERLLVRQQGKGTFVASYAGERSQFPFLRITPDAGPLQELT
                     AELLDLQRVRDPASARVLGLTATSGIYVLTRILALNGSPACYEEVRLPAPRFKGLTRA
                     VVEQYECMLYSMYETRFGVRMLHAEECVRAVPAPREVAAALHVPLGLPLLQIERIAYT
                     YGREPSELRRCYCDTREHHYRNAITG"
     gene            6579..6953
                     /gene="sdhC"
                     /locus_tag="VD791_06460"
     CDS             6579..6953
                     /gene="sdhC"
                     /locus_tag="VD791_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HXV07653.1"
                     /translation="MTDPRPKYLDLTRIRLPVPAVVSILHRASGAALFLLLPFLLYLL
                     HASLGSPDSYVRMRAAIEQPLVKLVLIGLLWAYLHHFCAGIRFLALDLHWGIALQPAR
                     RSARAVLAASLALTVVLGVWLW"
     gene            6947..7294
                     /gene="sdhD"
                     /locus_tag="VD791_06465"
     CDS             6947..7294
                     /gene="sdhD"
                     /locus_tag="VD791_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HXV07654.1"
                     /translation="MVKRIVVGAHYGLMSFLAQRITAAAMALYTLLILGVLLVAPPAH
                     YGHWKALFAQQWMKLATFVFLVSLFLHAWVGMRDIFMDYVKPTGWRLTAQAAVILLLI
                     AYAGWSIQILWGV"
     gene            7295..9058
                     /gene="sdhA"
                     /locus_tag="VD791_06470"
     CDS             7295..9058
                     /gene="sdhA"
                     /locus_tag="VD791_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HXV07655.1"
                     /translation="MGIATRKFDAVIVGAGGAGMRAALPLAEAGFRVAVLSKVFPTRS
                     HTVAAQGGIGAALGNMGEDSWLWHMYDTIKGSDYLGDQDSIEFLCRMAPQVVLELEHF
                     GMPFDRNPDGTVYQRPFGGHSQNFGERPVQRSCCAADRTGHAMLHTLYQRNLRANTHF
                     FVEWMALDLVRDASGQVLGVTALEMETGEMYLIHARATLFATGGAGRIYATSTNAFIN
                     TGDGLGMAARAGIPLEDMEFWQFHPSGVAGVGILITEGVRGEGGYLLNKGGERFMERY
                     APTMKDLASRDVVSRAMATEIKEGRGCGPEADYLLLKLDHLGSEVIDKRLPGIREIAR
                     TFANVDPVTDPIPVVPTVHYQMGGIPTNYHGEVIVPRGSHLEAVVPGFYAAGECACAS
                     VHGANRLGTNSLTDLLVFGKASGDRMVQFLRENPGHKDLPADAAERTRARLDRLENQR
                     AGENMYQVAAEMRRTMQAHCGVFRFPEMLAEGARKIREVAERAARTEIKDHSRVFNTA
                     RVEALELDNLIEVARASMVSAEARKESRGAHDRVDYPDRDDANWLKHTLWFKDGDRLM
                     YKPVHTKPITATTFEPKERTY"
BASE COUNT         1409 a         3045 c         3125 g         1528 t
ORIGIN      
        1 atcgaccggg aagaaaccca tggtggcgcc gtactcgggc gccatgttgg cgatggtggc
       61 gcggtcgggc acggccagat tctccacgcc ttcgccgaag aactcgacga acttgccgac
      121 gaccttggcc ttgcgcagca cctcggtgac ggtcaaaacc aggtcggtgg cggtcacccc
      181 gccgcgcagc cggcccttga gatgcacgcc caccacgtcc ggcgtcagga agtacaccgg
      241 ctgccccagc atcccggcct cggcctcgat gccgcccacg ccccagccca acacgccgat
      301 accgttgatc atcgtggtgt ggctgtcggt gcccaccagc gtgtccgggt agtacacgcc
      361 gtccttgcga tgcacgccgc gcgccaggtg ctccaggttc acctgatgca cgatgccgat
      421 gcccgggggc accaccttga aggtgtcgaa ggcctgcatg ccccacttca tgaactgata
      481 gcgctcgcgg ttgcgctcga actcaagctt catgttgagg tcgagggcct ttgcgctgcc
      541 gtaatggtcc acctgcaccg agtgatccac caccaggtcc accggcacca tcggttcgat
      601 cgacttgggg tccttgtaca tcttgtctgc gacgctgcgc atggccgcca ggtcggcgag
      661 caggggcacg ccggtgaagt cctgcagcac gatgcgggct accacgaacg ggatctcgtc
      721 cacgcgcgcc gcggccggtc gccagttcgc gagctgcttc acgtgtgctt cggtcacctt
      781 gccgccgtcg cagttgcgca gcaccgattc gagcacgatg cggatcgaca ccggcaggcg
      841 cgagatccgg cccactccgg ccttctcgag cgccggcagc gagtacagct tgccggtctt
      901 gcccggggcg atgtcgaagc tctgcagggt gcggtacgga ttgcgggtca tttgcgctct
      961 cgtcagatca cgaacaggtc gacgaactcg ttcaccgggg tgtcctcgag ccgctgctgg
     1021 tctttgcaca gcgccaggat gatcgccgcc tgcttcggcg gcaggcggcg cagcagattg
     1081 gtgcggaact tcgcctccag cagcgggatg ccttccgcgc ggcggcggcg gtgcccgatc
     1141 ggatactcga ccaccacctc gtcggtcttg gcaccgtcct tgaagtggat ggtgatgccg
     1201 ttggcgatgg agcgctggtc cgggtcgagg tagtccttgc tgaagcgttt gtcctccacg
     1261 cattccatct tttcgcgcag ggcgtcgatg cgcgggtcgg cggccacgtc gtcctcgtag
     1321 tcggccgccg tgaggcgccc gtggatcaag ggcaccgcga ccatgtactg gatacagtgg
     1381 tcgcggtcgg cggggttgtg cagcgggccc ttcttgtcga tgatgcggat ggccgactcg
     1441 tgggtgcgga tcacgatctt ctggatctga tccagtcggt cgcgcacttg cgcatgcagc
     1501 tgcatcgcgc actcgactgc ggtctgggcg tggaactcgg ccgggaacga gatcttgaac
     1561 agcacgtgct ccatcacgta gctgccatag ggccgctgca accggaacgc gttgcctttg
     1621 aacagcacgt cgtagaagcc ccaggtcttg gcgctcagga ccgacggata gcccatctca
     1681 ccctttatgg tgatgagcgc caggcgcacg gcgcggctgg tagcgtcgcc cgccgcccag
     1741 ctcttgcgcg agccggtgtt gggcgcgtgc cggtaggtgc gcaggctctg accgtcgacc
     1801 caggcctggg acacggcgtt gatgatctcg tcccgggttg cgcccagcat gcgcgccacc
     1861 actgccgtgc tcgccacctt gaccagcacc acgtggtcca gcccgacgcg gttgaagctg
     1921 ttctccagcg ccagcacgcc ctggatctcg tgcgccttga tcatggcggt gagcacgtcg
     1981 cgtatcgcga gcgggcgctt gccggcggcc agcgcgttgc gcgacagcca gtcggccacc
     2041 gccaggatgc cgcccaggtt gtcggacggg tgtccccatt cggcggcgag ccaggtgtcg
     2101 ttgaagtcga gccagcggat catggcgccg atgttgaacg cggcctgcac cgggtcgagc
     2161 tggaactggg tgccgggaac gcgtgcgccg ttcggcacta ccgtgcccgg cacgatcggc
     2221 ccgagcagct tggtgcaggc cgggtaggac agcgcttcca gcccgcagcc gagcgtgtcc
     2281 atcaggcaca ggcgagcggt gtcatacgcc tcctggctct tgatgtcgtg gtgcaggacg
     2341 tagtcggcga tgtcgaccag cggcgggtcg ggctcggggc ggacgttgga gacgggtgcg
     2401 gacatttaac ttcctgaaaa ccgcaaaggc gcagaggcgc aaaggagccg caaagcaaga
     2461 aaacggcttt cctttgcgca gcctttgcgt cttagcgcct ttgcggtcat cgctttactt
     2521 gcgcttttcg atcggcaccc acttgcggtc ttcgggcccg acatagttgg cgctcggccg
     2581 gatgatcttg ccgtcgaggc gctgctcgat cacgtgcgcc gaccagccgg aagtgcgcga
     2641 gatcacgaag atcggcgtga acatgggcgt cgggatgccc atcatgtgat agctggtggc
     2701 gctgaaccag tcgagattgg gaaacatcct cttctcgcgc cacatggtct cctccacccg
     2761 ggcggagatg tcgaacagct tcatgtcgcc ggcgtcctgg ctcaagcgcc gcgccacttc
     2821 cttgatcacc tggttgcgcg ggtcggagat ggtgtacacc gggtggccga agccgatgat
     2881 gatctgcttg tcggccacgc gcctcacgat atcggcttcg gcctcgtcgg gggtgtcgta
     2941 gcggccgatg acgtcgaagg cgaactcgtt ggcgccgccg tgcttgggtc ccttcagcgc
     3001 gccgatggcg ccggcgatgg ccgagtacag gtccgagccg gtgccggcga tcacgcgggc
     3061 ggcaaaggtg gaagcgttga actcatgctc ggcgtagagg ttgagcgagg tgtgcatggc
     3121 gcgcacccac aactcggggg gcggcttgcc gtgcagcagg tgcaggaagt gcccgccgat
     3181 ggagtcgtcg tcggtttcca cctcgatgcg ccggccggag tgcgagaagt gataccagta
     3241 cagcagcatg gagccgaacg aggccatcag ccggtcggcg acgttgcgcg cctcggcgat
     3301 cttgtggtcc acgttctcgg gcagcaccgc gcccagcgcc gagcagccgg tgcgcagcac
     3361 gtccatcggg tgggtgttgg ccgggatgca ctcgaggatg tcctgcaccg ccgcgggcag
     3421 gccgcgcagg gaccgcagtt tctgtttgta cgcggcgagc tccgccagcg tgggcagcct
     3481 gccgtgcacc agcaggtatg cgacctcctc gaactgcgcg cgctcggcga actcgaggat
     3541 gtcgtagccg cggtagtgca gatcgttgcc ctggcggccg acggtgcaca gccccgtgtt
     3601 gccggcgggc acgccggaca gcgcgacgga tttcttcggc ttgggtgcgg ttgcggcggg
     3661 cgcagcctgg ctcgtcattt gtgcttctcc ttggcgaaca gccgatcgag cttgtcttcg
     3721 taggcgtggt aatcgaggaa gtcgtacagc tcgtcgcggg tctgcatgct gtcgagcacg
     3781 ctcttctggg tgccttcgcg gcgcaccgtc cggtacacat tgagcgccgc ctggttcatc
     3841 gcgcggaacg ccgacagcgg gtacagcacc aggccgacgc cggccgagcg cagctcgtcg
     3901 gtggtgaaca gcggcgtctt gccgaactcc gtgatgttgg ccagcaccgg cacctgcact
     3961 gcatcggcga agcgccggta catgcccagg tcggtgaccg cctccggaaa gatcatgtcc
     4021 gccccggcct ccacgcaggc gcaggcgcgg tcgacggccg cctgcaggcc ctccacggcg
     4081 agcgcgtcgg tgcgcgccat gatcacgaac tgcgcatcgg tgcgggcgtc ggcggcggcc
     4141 ttgatgcggt ccaccatttc ctcgcgcggc acgatctcct ttcccggccg gtgcccgcag
     4201 cgcttggcct gcacctggtc ctcgatgtgc atggcgccgg cgccggactt gatcagcgat
     4261 ctcacggtgc gggcgatgtt gaaggcgccg ccgaatccgg tgtcggcatc caccagcagc
     4321 ggcacgccgc acacgtcggt gatgcggcgc acgtcggtca gcacgtcgtc cagggtgctg
     4381 atgcccagat cgggcagccc gagcgagccc gccgccacgc cgccaccgga caggtagatg
     4441 gcgcggtaac ccacgcgctc ggccagccga gcgtgatagg cgttgatcgt cccgatcacc
     4501 tgcagcgggg tttccgcctt gaccgccgcc ctgaacttcg cgcccgcgga tgtcatggct
     4561 tcatttcagc agcgacgcca ccgcgtcgcg ctcctcgagc agctccttgt gcgtggcgtc
     4621 catgcgcgcg cggctgaagg gactgacctc gatgtcctgc acgatccggt agcggccgcc
     4681 ggtgcaggtg accggatagc cgtagatcac accctgcggg atgccgtagc tgccgtccga
     4741 ggggatcccc atggtcaccc agtcgcccgg gcgtgtgccc cgaatccagt cgcgcatgtg
     4801 gtccatggcc gcgttggcgg cgctcgccgc ggacgaggcg ccgcgcgcgt cgatgatggc
     4861 ggcgccgcgc ttctgcacgg tgggaatgaa gctgccctcg agccaggcct ggtcggcgat
     4921 cagcggccac agcttctggc cgtccacctc ggcctgggac aggtcgggat actgggtggc
     4981 ggagtgattg ccccacacgg tgaccttgcg gagccccgcg accgggcggc cgaccttggc
     5041 ggcggcctgg gagacggcgc ggttgtggtc caggcgcatc atggcggtga aattgcccgg
     5101 atccaggccc ggcgcctggc tcatggcgat gtaggcgttg gtgttggccg gattgcccac
     5161 caccaacacc ttgacgtcgc ggctggcggc ctgatccagg gcgcggccct gctcgatgaa
     5221 gatcctggcg ttctccagca gcaggtcctt gcgctccatg cccggcccgc gcggccgcgc
     5281 gcccaccagc agggcgatgt ccgcgtcgcg gaaggcggcg tcggcgtcgg cgctggcgct
     5341 catgcccgcg agcagcggga aggcgcagtc gtcgagctcc atcatcacgc cgcggagcgc
     5401 cttctgcgcc ctgtcgtcgg ggatctcgag cagctgcagc aggaccggct gctcggcgcc
     5461 gagcatctcg ccggcggcga tgcggaacag caggctgtag ccgatctggc cggcggcgcc
     5521 ggtcaccgcg acgcgcacgg gtgctctcat tcgaggactc cggaaaagga acctaacagc
     5581 ctgctgcaga accagctcgc gggcgcacaa gtgcttgaac cggcggcgcg tcgcaggtcc
     5641 tgcgccgtcg ggcgtgccga gaaccgtgcg ctcggcggaa agcaactcga aataatagcc
     5701 aaccggccgc ggcggtgtca aaccactctt atgtcttata taagatacaa cactgtggac
     5761 gcgggcgcca tttcgtgcct agaatgacgc attcgtacaa gcgcctgccc ggatgaagcc
     5821 cgccgcggaa acccccgcct ttcaacccct gtaccggcag atcaagaccc tgatcacgcg
     5881 cagcctggtg tccggcgaat ggcgcccggg cgagtcgatc ccgagcgagg tcgagctcgc
     5941 cggccgctac agcgtcagcc agggcaccgt gcgcaaggcg gtcggcgagc tggccgagga
     6001 gaggctgctg gtgcgccagc aaggcaaggg cacgttcgtg gcctcctacg ccggcgagcg
     6061 cagccagttt ccgttcctgc gcatcacccc cgacgccggt ccgctgcagg agctgaccgc
     6121 cgagctgctc gatctgcagc gcgtgcgcga ccccgcctcg gcgcgcgtgc tggggctcac
     6181 cgccaccagc ggcatctacg tgctcacgcg catactggcg ctcaatggca gcccggcgtg
     6241 ctacgaggag gtacgactgc cggccccgcg cttcaagggg ctgacgcgcg cggtggtcga
     6301 gcagtacgag tgcatgctgt acagcatgta cgagacgcgc ttcggcgtgc gcatgctgca
     6361 cgcggaagaa tgcgtgcggg cggtgccggc tccgcgcgaa gtggccgccg cgctgcacgt
     6421 tcccttgggc ctgccgctgc tgcagatcga gcgcatcgcc tacacctacg ggcgcgagcc
     6481 ctcggagctg aggcgctgct actgcgacac ccgcgagcac cactaccgca acgcgatcac
     6541 cggttgaccg taccatgcag gcctccaggg acccgccgat gaccgacccc cgacccaagt
     6601 acctcgacct cacgcgcatc cggctgccgg tgcctgctgt ggtgtccatc ctgcatcgcg
     6661 cgagcggcgc ggctctgttt ctgctgctgc cctttctgct gtacctgctg cacgccagcc
     6721 tgggctcgcc ggacagctac gtgcggatgc gcgcggcgat cgagcaaccg ctggtgaagc
     6781 tggtgctgat cgggttgctg tgggcttacc tgcaccattt ctgcgccggc atccgctttc
     6841 tggccctgga cctgcactgg ggcatcgcgc tgcagcccgc gcgccgcagc gcccgggcgg
     6901 tgctggcggc gagcctcgcg ctgaccgtcg tcctgggagt gtggctgtgg tgaagcgcat
     6961 cgtcgtcggc gcccattacg gcctgatgag cttcctggcg cagcgcatca cggccgcagc
     7021 gatggcgctc tacacgctgc tgatcctggg cgtgctgctg gtcgcgccgc cggcccacta
     7081 cggccactgg aaggcgctgt tcgcgcagca gtggatgaaa ctcgccactt tcgtgttcct
     7141 ggtgagcctg ttcctgcacg cctgggtggg catgcgcgat atcttcatgg actacgtcaa
     7201 gcccaccggc tggcggctga cggcgcaggc ggcggtgatc ctgctgctca tcgcgtacgc
     7261 cggctggtcc atccagatcc tttggggggt gtgaatgggg atcgccacgc gcaagttcga
     7321 cgcagtgatc gtgggggcgg gcggcgccgg catgcgcgcc gcgctgccgc tcgccgaggc
     7381 tgggtttcgg gtcgcggtcc tgtccaaagt gtttccgacc cgctcgcaca cggtggcggc
     7441 gcagggcggc atcggcgccg cgctcggcaa catgggcgag gacagctggc tgtggcacat
     7501 gtacgacacc atcaagggtt cggactatct gggcgaccag gattcgatcg agttcctgtg
     7561 ccggatggcg ccgcaggtgg tgctcgagct ggagcacttc ggcatgccgt tcgatcgcaa
     7621 ccccgacggc accgtgtacc agcgcccctt cggcgggcat tcgcagaact tcggcgagcg
     7681 cccggtgcag cgcagctgct gcgccgccga ccgcaccggc cacgccatgc tgcacacgct
     7741 gtaccagcgc aacctgcgcg ccaacaccca tttcttcgtc gagtggatgg cgctggacct
     7801 ggtgcgtgac gccagcgggc aggtgctggg cgtcaccgcc ctggagatgg aaaccggcga
     7861 gatgtatctg atccacgcgc gcgcgacgct gttcgccacc ggcggggcag ggcgcatcta
     7921 cgccaccagc accaacgcct tcatcaacac cggcgacggg ctgggcatgg cggcgcgcgc
     7981 cggcatcccg ctggaggaca tggagttctg gcagttccac ccgagcggcg tggccggggt
     8041 ggggatcctg atcaccgagg gcgtgcgcgg ggagggcggt tacctgctga acaagggcgg
     8101 cgagcgcttc atggaacgct atgcgcccac catgaaggat ctggccagcc gcgacgtggt
     8161 gtcgcgcgcc atggccaccg agatcaagga gggccgcggc tgcggccccg aggccgatta
     8221 cctgctgctc aagctcgacc acctcggatc cgaggtcatc gacaagcgcc tgccgggcat
     8281 tcgcgagatc gccaggacct tcgccaatgt cgatcccgtc accgacccga tcccggtggt
     8341 gcccaccgtg cactatcaga tgggcggcat ccccaccaac taccatggcg aggtaatagt
     8401 gccccggggc agccacctcg aagcggtggt ccccggtttc tacgccgcgg gcgagtgcgc
     8461 ctgcgcctcg gtgcacgggg ccaaccggct gggcaccaac tcgctcaccg acctgctggt
     8521 gttcggcaag gcgagcggcg atcgcatggt gcagttcctg cgcgagaacc ccgggcacaa
     8581 ggacctgccc gccgacgccg ccgagcgcac gcgggcgcgc ctcgaccgcc tggagaacca
     8641 gagggccggc gagaacatgt atcaggtcgc cgccgagatg cgccggacca tgcaagccca
     8701 ctgcggcgtg tttcgcttcc cggagatgct ggccgagggc gcgcgcaaga tccgcgaggt
     8761 cgccgagcgg gccgcgcgca ccgaaatcaa ggaccacagc agggtgttca ataccgcgcg
     8821 cgtcgaggcg ctcgaactgg acaacctgat cgaggtggcg cgcgccagca tggtttcggc
     8881 cgaggcgcgc aaggagagcc gcggggcgca cgaccgcgtc gattaccccg accgcgacga
     8941 cgcgaactgg ctcaagcaca cgctatggtt caaggacggc gaccggctga tgtacaagcc
     9001 ggtgcacacg aagccgatca ccgcgacgac gttcgagccg aaggagagga cttattgaac
     9061 cacagaggca cagagacaca gaggacttcg aatccgggat tccgggt
//
LOCUS       DASPGE010000201         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_55322, whole genome shotgun sequence.
ACCESSION   DASPGE010000201 DASPGE010000000
VERSION     DASPGE010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55322"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /gene="cax"
                     /locus_tag="VD791_06475"
     CDS             <1..1005
                     /gene="cax"
                     /locus_tag="VD791_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HXV07656.1"
                     /translation="PEAHTVLFVLSVLAIVPLAALLSHATESVAAKTGDAAGGLLNAT
                     LGNLTELVIALTALRAGQYMLVKASIAGAIVTNTLFMLGASFLLGGLKHHVQEYNRVS
                     ARLQAGLLFLATIALLVPSTVSVADGAAGATVIRELSVGLALLLIVAYGLGMLFSLKT
                     HRELFASAYSAQTGEAPWPIGLALATLAVVTGLVALVSEVFVESVQQAAMEFGMTPAF
                     VGFIVVALVGGAAEMASAFSGARKNRLDLSVGIALGSASQIALFVAPVLVLLSYVIGP
                     APMDLQFWPGAVVMMLIATLTASLVTNSGRSAWFVGVLVLMVYLVFALSLYLLPPRAQ
                     "
     gene            1055..1717
                     /locus_tag="VD791_06480"
     CDS             1055..1717
                     /locus_tag="VD791_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HXV07657.1"
                     /translation="MSEHPSKSAKRVLDPIDRVSEILFGLIMVLTFTGSLSIAEAGRD
                     DVRTMLIGALGCNLAWGIIDAVLYLMGCLAEKGRGLATLRATRNAADPRQAQRLIADA
                     LPPLVASILEPAELESMRQRLQRLPEPPAHARLRKDDWLGAVGVFFLVFLSMLPVAVP
                     FMFMHNAVPALRVSNGIAIVMLFSLGYAFGGMTGRRPWAVGISMVLLGALLVALAIAL
                     GG"
     gene            1720..>2096
                     /locus_tag="VD791_06485"
     CDS             1720..>2096
                     /locus_tag="VD791_06485"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXV07658.1"
                     /translation="MKAVTVVPKTPRSARLEEVPEPHARDGSVLVEAIAVGVCGTDTE
                     IVEGKYGWAPPDKTRLVLGHESLGRVIDPGPASALKAGELVVGIVRRPDPVPCPNCAV
                     GEWDMCRNGQYTERGIKQIDGFMS"
BASE COUNT          291 a          694 c          730 g          381 t
ORIGIN      
        1 cccgaagcgc acacggtgct cttcgtgctg tccgtgctcg ctatcgtgcc gcttgccgca
       61 ctgctgagcc atgccaccga atcggtcgcg gccaagacgg gcgatgcggc gggcgggctg
      121 ctcaacgcca cgctggggaa cctgaccgaa ctggtgatcg ccctgaccgc gctgcgcgcc
      181 ggccagtaca tgctggtgaa ggcgtccatc gccggcgcga tcgtcaccaa caccctgttc
      241 atgctgggtg cgtcgtttct cctcggcggg ctcaagcacc atgtgcagga atacaaccgg
      301 gtcagcgccc gcctgcaagc gggactgctg ttcctggcca cgatcgcgtt gctggtccca
      361 tcgacggtct ccgtggccga cggggcggcg ggcgccacgg tcatccgcga gctgagcgtt
      421 gggctggcgc tgctgctcat cgtcgcctac gggctgggta tgctgttctc gctcaagacg
      481 caccgcgaac tgtttgccag cgcgtattcc gcgcaaacgg gcgaagcgcc atggccgatc
      541 ggcctggccc tggctacgct ggcagtcgtc acggggctgg ttgcgctggt gagcgaagtg
      601 ttcgtcgagt cggtgcagca ggcggcgatg gaattcggga tgacgcccgc gttcgtgggt
      661 ttcatcgtcg tggcgctggt agggggcgcg gcggagatgg cctcggcgtt ttccggcgcg
      721 cggaagaatc gactcgacct gagcgtgggc atcgcgctgg gcagcgcgtc tcagatcgcg
      781 ctgttcgtgg cgccggtgct ggtgctgctg agctacgtca tcggtccggc gccgatggac
      841 ctgcaattct ggccgggcgc ggtggtgatg atgctcatcg cgaccctcac cgcctcgctg
      901 gtgaccaaca gcgggcgatc tgcgtggttc gtcggcgtcc tcgtgctgat ggtgtacctg
      961 gtgttcgcct tgagccttta cttgttgccg ccccgagcgc aatgaaccgc ccgtccgcgg
     1021 aaaggtagcc ggcagcgcgc cgcccgccac gcccatgtcc gaacacccca gcaagtccgc
     1081 caaacgggtt ctggatccga tcgatcgcgt ctccgagatc ctgttcgggc tgatcatggt
     1141 cctcacattc accggttcgc tcagcatcgc ggaggccggg cgcgacgacg ttcgcacgat
     1201 gctgatcggc gcgctcggct gcaacctggc gtggggcatc atcgacgcgg tgctgtacct
     1261 catgggctgc ctcgccgaaa aaggccgcgg cctcgcgacc ttgcgcgcta cgcgcaacgc
     1321 cgccgatccg cgacaggcgc agcgcctcat cgcggacgcc ctgccgccgc tggtcgcttc
     1381 catcctggag ccggcagagc tcgaatccat gcgccagaga ttgcagcgac tgcccgagcc
     1441 gcccgcgcac gcacgcttgc gcaaggacga ctggctgggc gccgtcggcg tctttttcct
     1501 ggtgttcctg tccatgttgc cggtggcggt gccgttcatg ttcatgcaca acgccgtgcc
     1561 ggcgttgcgt gtctccaacg gcatcgccat cgtcatgctg ttctcgttgg gttacgcctt
     1621 cgggggcatg accgggcgtc gcccgtgggc ggtgggcatc tccatggtgc tcctcggggc
     1681 gctgctggtg gcgctggcca tcgcgctggg aggatgaaga tgaaagccgt taccgtcgtg
     1741 ccgaagacgc cgcgcagcgc gcggctggag gaagtcccgg agccgcatgc gcgcgacggc
     1801 tcggtcctgg tcgaggcgat cgccgtcggc gtgtgcggca ccgacaccga gatcgtcgaa
     1861 ggcaagtacg gctgggcgcc gcccgacaag acgcggctgg tcctcggcca cgaatcgctc
     1921 ggccgcgtga tcgatcccgg gccggcgagc gcgctgaaag cgggcgagtt ggtggtcgga
     1981 atcgtgcgcc ggcccgatcc cgtcccctgc cccaactgcg ccgtgggcga gtgggacatg
     2041 tgccgcaacg ggcagtacac cgagcgcggc atcaagcaga tcgacggctt catgtc
//
LOCUS       DASPGE010000202        11110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1342579, whole genome shotgun sequence.
ACCESSION   DASPGE010000202 DASPGE010000000
VERSION     DASPGE010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11110
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342579"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1716)
                     /locus_tag="VD791_06490"
     CDS             complement(112..1716)
                     /locus_tag="VD791_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HXV07659.1"
                     /translation="MGKPDMGGFGWDFPYFSQRMPILAANVVATSQPLAAQAGMRMLL
                     KGGNAADAIVATAITLTVVEPTMNGIGSDAFALLWDGDRLHGLNASGRAPAAWTPERF
                     AGRTDMPTVGWDSVTVPGVVSAWVEVSRRFGKLPFAALFEPAIEYAERGYLVAPMIAR
                     QWAGQIERLAEQPGFAQAFMPGGRAPLAGERFRSPEQASTLRQIADTGGEAFYRGALA
                     EQIAAASRAGGGAMTTADLAEHRADWVEPISQPYRALRLHEIPPNGQGIAALMALGIL
                     QHFDLGSLAPESTETLHLQIEAMKLAFADAYRWVADPRHMPVAAADLLDPGYLKQRAA
                     RIDRSRAQEHGPGAPPKSGTVYLTAADADGMMVSFIQSNYKGFGSGVVVPGTGISLQN
                     RGMGFKLQAGHPNRVGPRKRPFHTIIPGFVTRDGASLMSFGVMGADMQPQGHVQMLVR
                     LADYRQNPQAAADAPRWKVLPDGSIIVEHHMPAAAVAGLAERGHRIQQTERWSIEYGA
                     AQLIHRVDGGYVAASEPRRDGQAVGF"
     gene            complement(1748..3250)
                     /locus_tag="VD791_06495"
     CDS             complement(1748..3250)
                     /locus_tag="VD791_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXV07660.1"
                     /translation="MELLANLTTGFSVALTWHNVAFCFIGVVLGTLVGVLPGIGPVAT
                     IAMLLPITFHFEPASALIMLAGIYYGAQYGGSTTAILVNLPGEVSSVVTTLDGYQMAR
                     QGRAGPALATAALGSFFAGCVATLVLATFAPPLAELAFKFGPAERFSLMVLGLITAVT
                     LAHGSLLKAVAMIVLGILLGLVGADPEVGRMRFTFGIPQLTDGIGVAVVAMGIFAFGE
                     IIANLERTGAREVYASNVKGVWLSREDFRLAWKAVLRGTAIGSLLGILPGGGAVLGAF
                     GSYMVEKKLARDPSRFGKGAIEGVAGPESANNAGAQTSFIPLLTLGIPSNAVMALMVG
                     AMIIHGIQPGPQVMSERPELFWGMIASMWVGNLLLVILNLPLIGIWVKLLAVPYRMLY
                     PSIMVLCCIGIYSLNNSVFDVGVAVLFGLLGYVFIKLKCEPAPLLLGFVLGPMLEENL
                     RKAMTISRGDPSVFFTRPLSLSMLVVAAVLVLIMILPVVRRRRAEAFQEA"
     gene            complement(3250..3699)
                     /locus_tag="VD791_06500"
     CDS             complement(3250..3699)
                     /locus_tag="VD791_06500"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXV07661.1"
                     /translation="MGFGIRSRQDFLAGLLFVFFGAFALVLARNYPLGTAMRMGPGYF
                     PTILGGMLVALGGAVIVRAFVLPGDKLGGVGLMPLLLVLASVALFAVTVERFGIVLSV
                     VLVVFVSSLASGRFRWLEVGLLAAVMVALGVGLFTKGLGLPFKIFPG"
     gene            complement(3949..4758)
                     /locus_tag="VD791_06505"
     CDS             complement(3949..4758)
                     /locus_tag="VD791_06505"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HXV07662.1"
                     /translation="MAHTRNRLGLRGAIGGPFLAVLAWTWAWVGDALGACTIESPPHR
                     ATVLELYTSEGCNSCPPADRWLSRLREQGPEPKVLPLAFHVDYWNELGWVDRFSRPAF
                     SARQRQVAARHRGGIIYTPQMVLDGQDWRGWRSDDLVARLRSINGERPGATIRADALI
                     SGLGVQVQGTARLADRKQAGVVAAWLALYEQGLVTAVAAGENRGRTLQHDYVVRALIG
                     PLRAGADGSIALSGSIPLSADWKRERLGIAAFAEHNVTGRVLQAASAAGCL"
     gene            4942..5571
                     /locus_tag="VD791_06510"
     CDS             4942..5571
                     /locus_tag="VD791_06510"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HXV07663.1"
                     /translation="MAQYFVIHPQDPQRRLLQQAAQIVRQGGLIVYPTDSCYALGCQI
                     GDKAAQERMRAIRELAPEHHFTLVCRDLSEIAHYARVDNRQYRLLKATTPGSYTFILQ
                     ATREVPRRLQNPRRNTIGLRVPDHKVVQALLAELDAPLLSSTLLLPGDELPLNDAGEI
                     RERLERQVELILDGGPCGIEPTTVVDLTSEAPEVVRAGKGSLEPFGVTA"
     gene            5580..6254
                     /locus_tag="VD791_06515"
     CDS             5580..6254
                     /locus_tag="VD791_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXV07664.1"
                     /translation="MNFDLAGLIQLIALAALPVTFAITLHEAAHGYAARHFGDYTAAN
                     AGRITLNPLRHIDPVGTIAVPLLILVMSKLLGGGGILFGWAKPVPVNFGQLRSPKRDM
                     LWVAAAGPGANLFMAACWALVLKFADGLPWNYFSAPLLGMAQIGISINVVLMVLNLFP
                     LPPLDGGRIAVSLLPSRVAWRFAMLEPYGFMILLVLLFTGVLGAVLSPFIRLVLGALD
                     ALFNLR"
     gene            6261..7463
                     /locus_tag="VD791_06520"
     CDS             6261..7463
                     /locus_tag="VD791_06520"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HXV07665.1"
                     /translation="MFPDRVLSGMRPTGSLHLGHYHGVLKNWIRLQHEHPCLFFVADW
                     HALTTHYEEPGIIEANTWEMVIDWLAAGVDPSLATLFIQSRVPEHAELHLLMSMITPL
                     GWLERVPSYKEQQEKLSDRDLATYGFLGYPLLQSADILIYRATLVPVGEDQVPHIEFT
                     REIARRFNHIYGREPGFEEKAEAAVKKLGSKRARLYRELRARYQEQGDHEALEAGRAL
                     LGEAQNLSMGDQERLFGYLEGGGKMILSEPEALLTEASKMPGLDGQKMSKSYNNTIGL
                     REDADSVTRKIRTMPTDPARVRRSDPGEPEKCPVWQFHLVYSDEPTREWVQKGCRSAG
                     IGCLECKQPVIDAVLREQEPMRERAQAYVDDPTLVRNIIEDGCERARKEAGETMREVR
                     EAMGLAQG"
     gene            complement(7522..7968)
                     /gene="smpB"
                     /locus_tag="VD791_06525"
     CDS             complement(7522..7968)
                     /gene="smpB"
                     /locus_tag="VD791_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HXV07666.1"
                     /translation="MSIAQNRKAYHEYFIEEKYEAGIMLEGWEVKAIRAGQVHLSEAY
                     VILKGGAPYLIGCHITPLKTASTHVQPDPTRTRKLLLNAEELRRLIGKVERAGYALVP
                     LDMHYRGGWIKLEIGLAKGKKQHDKRQAEKERDWQREKQRLLRQRA"
     gene            8106..8543
                     /locus_tag="VD791_06530"
     CDS             8106..8543
                     /locus_tag="VD791_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HXV07667.1"
                     /translation="MALVQRSVLVGYAAEKMYALVDQVEHYPEFLPWCGRTHVDYRDG
                     ALTRASILIDFRGIKQAFQTENRTEPPHLIEIKLVSGPFRTLDGTWRFQPLAVDACRV
                     DFRLHYEFSSKMLERLVGPVFHYIASSFIDAFVKRAERLYGKG"
     gene            8540..8833
                     /locus_tag="VD791_06535"
     CDS             8540..8833
                     /locus_tag="VD791_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010092084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HXV07668.1"
                     /translation="MTLGETLTVEVVFALPLEQDCAVVELPDGASVRDAIARSGVTAR
                     HPIIDLGACRVGIWGRPAGLDDRVRNGDRVEIYRPLQADPKESRRTRAKRTRS"
     gene            complement(8856..9341)
                     /locus_tag="VD791_06540"
     CDS             complement(8856..9341)
                     /locus_tag="VD791_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HXV07669.1"
                     /translation="MKWIATLALLALALPAWAAEMYKWVDETGKVHYSDQPPPANARQ
                     QQTIQPKVRAQGAATTPAAPEGQAAPATKAKTAAELDMEFRKRRTEAAEAEAKRQQEA
                     QAATEKQRNCEAAKARVAVLQTGGRITKASPSGEQIYLGDNEIAAELIEAQKVADSWC
                     K"
     gene            complement(9354..9620)
                     /locus_tag="VD791_06545"
     CDS             complement(9354..9620)
                     /locus_tag="VD791_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07670.1"
                     /translation="MHAGDAKPGSAGVNDPGAEQARREVLRAQQAVQAAAEKRALWTT
                     AQEALAEAEAALARGDHAAAVQASRAAAEQARLGIEQLDYPRFP"
     gene            9782..>11110
                     /gene="guaB"
                     /locus_tag="VD791_06550"
     CDS             9782..>11110
                     /gene="guaB"
                     /locus_tag="VD791_06550"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HXV07671.1"
                     /translation="MRVVERALTFDDVSILPSHSIVLPRDVSLKTRLTRAIELNLPLV
                     SAAMDTVTEARLAIAMAQEGGVGIVHKNMAPRAQAAEVAKVKRYESGVVKDPITIAPD
                     MSVREVLSLIRAHKISGLPVVEGKRVVGIVTNRDLRFETNLDQRVRDIMTPRERLVTV
                     REGGSLEEAQALMHKHRLERVLVVNANFELRGLITVKDILKASEYPNACKDELGRLRV
                     GAAIGVGEGTEERAEALVEAGADVLVVDTAHGHSEGVLERVRWARRQFPRMQIVAGNI
                     ATAAAAKALVDAGADAVKVGIGPGSICTTRIVAGVGVPQITAIANVALALKTTDVPLI
                     ADGGIRYSGDVAKAIAAGADAVMLGGLFAGTEEAPGEIELFQGRSYKSYRGMGSLAAM
                     TQGSSDRYFQDAADSVEALKLVPEGVEGRVPYKGSLIQVIEQLMGGLRSSM"
BASE COUNT         1733 a         3832 c         3840 g         1705 t
ORIGIN      
        1 cgccgcggcc gccgggcgcc cggcggtgct ggagctgcgc atcgaccccg aagcgatctc
       61 cacgcgcacc acgctgacgc aggtccgcga gcaggcgctc aaggcgcggc gctagaaacc
      121 gactgcctgg ccgtcgcgcc gcggctccga ggccgccacg tagccgccgt cgacgcggtg
      181 gatgagctgc gcggcgccgt attcgatgct ccaacgctcg gtctgctgga tgcggtggcc
      241 gcgctcggcg agccccgcga cggccgcggc gggcatgtgg tgctcgacga tgatgctgcc
      301 gtcaggcagc accttccagc gcggcgcgtc ggccgcggcc tgcgggttct ggcgatagtc
      361 ggccaggcgc accagcatct gcacatgccc ctgcggttgc atgtcggcgc ccatcacgcc
      421 gaagctcatc agcgacgcgc cgtcgcgcgt gacgaaaccc gggatgatgg tgtggaaggg
      481 gcgcttgcgc ggcccgaccc ggttgggatg cccggcctgc agcttgaagc ccatgccacg
      541 gttctgcagg ctgatgccgg tgccgggcac caccactccc gagccgaagc ccttgtagtt
      601 cgactggatg aaggacacca tcatgccatc ggcatcggct gcggtgagat acaccgtgcc
      661 cgatttcggc ggcgcgcctg gtccgtgctc ctgcgcgcgg ctgcggtcga tgcgcgcggc
      721 gcgctgcttc agatagccgg gatcgagcag gtcggcggcc gccaccggca tgtgccgcgg
      781 atcggcgacc cagcgatagg cgtcggcgaa cgcgagcttc atcgcctcga tctgcaaatg
      841 cagcgtttcg gtcgattcgg gcgcgaggct gcctagatcg aaatgctgca ggatgcccag
      901 cgccatcagc gccgcgatgc cctggccgtt gggcgggatc tcgtgcaggc gcagcgcgcg
      961 gtacggctgg gagatcggct ccacccagtc ggcccggtgc tcggccagat cggccgtggt
     1021 catcgctccg cctccggccc ggctcgcggc ggcgatctgc tcggccagcg caccgcgata
     1081 gaacgcctcg ccgccggtgt cggcgatctg gcgcagggtg ctcgcctgct ccggcgaacg
     1141 gaaacgctcg ccggcgagcg gtgcgcggcc gcccggcatg aacgcctgcg cgaagcccgg
     1201 ctgctcggcg aggcgctcga tctgtccggc ccattggcgt gcgatcatcg gggcgacgag
     1261 gtacccgcgc tcggcgtatt cgatggccgg ctcgaacagc gcggcgaacg gcagcttgcc
     1321 gaagcgccgc gacacctcca cccatgcgga caccacgccg ggcacggtga ccgagtccca
     1381 gcccacagtc ggcatgtcgg tgcgcccggc gaagcgctcg ggcgtccacg ccgccggcgc
     1441 gcgccccgaa gcgttcaggc cgtgcagccg gtcgccgtcc cacagcagcg cgaaggcgtc
     1501 ggagccgatg ccgttcatgg tcggctcgac cacggtcagc gtgatggcgg tggcgacgat
     1561 ggcgtcggcg gcgttgccgc ccttgagcag catgcgcatg cccgcctgcg cggcgagcgg
     1621 ctgcgaggtg gcgacgacgt tcgccgccag gatcggcatg cgctgcgaaa agtacggaaa
     1681 gtcccagccg aaaccaccca tatcaggctt ccccatctca ggcttcccca tctcaagctt
     1741 ccccgtgtca ggcttcctgg aacgcctccg cgcgccggcg gcgcacgaca ggcaggatca
     1801 tgatcaagac caggaccgcg gccactacca gcatgctcag gctgagcgga cgcgtgaaga
     1861 acacgctcgg gtcgccgcgc gagatggtca tcgccttgcg caggttctcc tcgagcattg
     1921 ggccgagcac gaacccgagc agcagcggcg cgggctcgca cttgagcttg atgaacacgt
     1981 agcccagcag tccgaacagc accgccacgc cgacgtcgaa cacggagttg ttcaagctgt
     2041 agatgccgat gcagcacagc accatgatcg acgggtagag catgcggtac ggcacggcga
     2101 gcagcttcac ccagatgccg atcagcggca gattgaggat caccagcaac aggtttccca
     2161 cccacatgct ggcgatcatc ccccagaaca gctccggccg ctccgacatc acttgcgggc
     2221 cgggctggat gccgtggatg atcatcgcgc cgaccatcag cgccatcacg gcattggacg
     2281 ggatgcccag cgtcagcagc ggaatgaacg aggtctgggc gccggcgttg ttggccgact
     2341 ccggcccggc gacgccctcg atcgcgccct tgccgaagcg cgagggatcg cgcgccagct
     2401 tcttctccac catgtaggag ccgaaggcac cgagcacggc gccgcctccg ggcaggatgc
     2461 cgagcagcga gccgatggcg gtgccgcgca gcaccgcttt ccaggccagc cggaaatcct
     2521 cgcgcgacag ccacacgcct ttcacgttgg aggcgtacac ctcgcgcgcg ccggtgcgct
     2581 ccaggttggc gatgatctcg ccgaaggcga agatgcccat cgccactacg gccacaccga
     2641 tgccgtcggt gagctgcgga atgccgaagg tgaagcgcat gcgcccgacc tccgggtcgg
     2701 cgcccaccag gccgagcagg atgcccagca cgatcatggc gaccgccttg agcagcgagc
     2761 cgtgcgccag cgtcaccgcc gtgatcagcc ctagcaccat cagcgagaag cgctcggccg
     2821 gcccgaactt gaaggccagc tcggcgagcg gcggcgcgaa ggtcgcgagg accagcgtcg
     2881 cgacgcaacc ggcgaaaaaa gatcccaggg cggccgtcgc cagcgccggg cccgcgcgcc
     2941 cctggcgcgc catctggtag ccgtccagcg tggtgaccac cgaggacact tcgcccggca
     3001 gattgaccag gatcgcggtg gtcgagcccc cgtactgtgc cccgtagtag atgccggcga
     3061 gcatgatcag cgccgaggcc ggctcgaaat ggaaagtgat cggcagcagc atggcgatgg
     3121 tcgccaccgg cccgatgccg gggagcaccc ccaccagcgt gcccagcacc acgccgatga
     3181 agcagaaggc gacgttgtgc caggtgaggg cgacggagaa ccccgtcgtc aggttcgcca
     3241 gcagctccat caaccgggga aaatcttgaa gggcagaccg aggcccttgg tgaacaggcc
     3301 cacgcccagc gccaccatga ccgcggcgag cagaccgacc tcgagccacc tgaagcgtcc
     3361 gctcgccagc gagctgacga acaccaccag caccaccgac aggacgatgc cgaagcgttc
     3421 gaccgtgacc gcgaacagcg ccaccgaggc gagcaccagc agcagaggca tcagcccgac
     3481 gccgccgagc ttgtcgccgg gcaggacgaa agcgcgcacg atcaccgccc ctcccagcgc
     3541 caccagcatg ccgccgagga tcgtggggaa gtaccccggc cccatgcgca tcgccgtgcc
     3601 gaggggataa ttgcgcgcta ggaccagggc gaacgcaccg aagaacacga acagcaggcc
     3661 cgccaggaag tcctgcctgc tgcggatgcc gaagcccaac gcctcctcct gtcccgttct
     3721 tgtcgttctg tgcatctgcg gccggctgcc cgctgcgttc gagccgtgtc gattcgcgac
     3781 gggccgacgc gtcgtttcac gaaccggcgc cgggggattg tttcacagac cggcgcgggc
     3841 gggcaaaccg gctggtcgcc gcctcgcgcg tagcccggat gcagcgcagc ggaatccggg
     3901 ggaagctgga gcctttttcc tccccgatct tgggtggctg catccaggct acaggcagcc
     3961 ggctgccgat gcggcctgca gcacgcggcc ggtgacgttg tgctcggcga aggcggcgat
     4021 gcccaggcgc tcgcgtttcc agtccgcgct cagggggatg ctgccgctga gcgcgatcga
     4081 cccgtcggcg cccgcacgca gcggcccgat cagggcgcgc accacgtagt cgtgctgcaa
     4141 cgtgcgcccg cggttctcgc ccgcggccac ggcggtgacc aggccttgct cgtagagcgc
     4201 gagccaggcg gcgaccacgc cggcctgctt gcggtcggcg agccgggcgg tgccttgcac
     4261 ctggacgccg agcccgctga tgagcgcatc ggcgcggatg gtggcgccgg gccgttcgcc
     4321 gttgatgctg cgcagccgcg caaccaggtc gtcgctgcgc cagccgcgcc aatcctgccc
     4381 gtccaggacc atctgcgggg tgtagatgat gccgccgcga tggcgggccg cgacctggcg
     4441 ctgccgcgcg ctgaaggcgg gtcgggagaa gcgatccacc cagcccagct cgttccagta
     4501 atcgacgtgg aaggcgagcg gcagcacttt cggttcgggc ccctgctcgc gcagacggct
     4561 cagccagcgg tcggccgggg ggcagctgtt gcaaccctcc gaggtgtaca gctcgagcac
     4621 ggtggcgcgg tgcggcgggc tctcgatggt gcacgcgccg agcgcgtcgc ccacccaggc
     4681 ccaggtccag gcgagcacag cgagaaaagg gccaccgatc gcgccgcgga ggccgagccg
     4741 gtttcgggtg tgtgccattt ctgctctcct ggccgccgcc ggccttttgg ggatgcgccc
     4801 gatttgctac cattgcgcgc ttctgcgcgc tccgcgcagg gcttcccgcc ggggcgcgcg
     4861 ccggtggcgg cctccatgct atacgcgggc gcccctctgc ccggatgcag tggggccgat
     4921 tgcggcaggg cgcgccaccc gatggcccag tactttgtca tccatccaca ggatccgcag
     4981 cggcgtttgc tgcagcaggc ggcccagatc gtacgccagg gaggtctgat cgtgtacccg
     5041 acggactcct gctatgcgct gggctgtcag atcggcgaca aggccgcgca ggaacgcatg
     5101 cgcgcgatcc gggagctcgc cccggagcac catttcacgc tggtgtgccg tgacctctcc
     5161 gagattgccc actacgcccg cgtcgacaac cgccagtatc ggctgctcaa ggcgaccacg
     5221 cccggcagct acaccttcat tctccaggcc acgcgcgagg tgccgcggcg cctgcagaac
     5281 ccgcggcgca acaccatcgg cctgcgcgtg cccgatcaca aggtggttca ggcgctgctc
     5341 gcggagctgg atgcgcccct gctgagttcc acgctgctcc tgcccggtga cgagctgccg
     5401 ctcaacgacg ccggcgagat ccgcgagcgg ctggagcgcc aggtcgagct gatcctcgac
     5461 ggcggtccct gcggcatcga acccaccacc gtggtggacc tcacttcgga ggcgcccgaa
     5521 gtggtgcgtg cggggaaggg cagcctcgag cccttcggcg tgaccgcctg atcttggcga
     5581 tgaacttcga tctcgccggc ctgatccagc tcatcgccct ggcggcgctg ccggtgacct
     5641 tcgccatcac gctgcacgag gccgcgcacg gctatgccgc gcgccacttc ggcgactaca
     5701 ccgcggccaa cgccgggcgc atcacgctca acccgctgcg ccacatcgat ccggtgggca
     5761 cgatcgccgt cccgctgctg atcctggtga tgagcaagct gctgggaggc ggcgggatcc
     5821 tgtttggctg ggccaagccg gtgccggtga acttcggaca gctgcgcagc cccaagcgcg
     5881 acatgctgtg ggtggcggcg gcggggcccg gcgccaacct gttcatggcc gcgtgctggg
     5941 cgctggtcct gaagttcgcc gacggcttgc cctggaacta tttttcggcg ccgctgctgg
     6001 ggatggccca gatcggcatc agcatcaacg tggtgctgat ggtgctcaac ctgtttccgc
     6061 tgccgccgct ggacggcggc cgcatcgcgg tcagcctgct gccgagccgc gttgcctggc
     6121 gcttcgcgat gctcgagccc tacggtttca tgatcctgct ggtgctgctg ttcacgggcg
     6181 tgctcggcgc ggtgctgtcg ccgttcatca ggctggtgct cggcgcgctc gacgcgctct
     6241 tcaatctgcg ctgaccgccc atgttccccg atcgcgttct gtccggcatg cggcccaccg
     6301 gcagcctgca tctcggccac taccacggcg tgctgaagaa ctggatccgg ctacagcacg
     6361 agcatccgtg cctgttcttc gtggccgact ggcatgcgct caccacccac tacgaggagc
     6421 cgggcatcat cgaggcgaac acgtgggaga tggtcatcga ctggctggcc gcgggcgtgg
     6481 acccgtcgct ggccacgctg ttcatccagt cgcgcgtgcc ggagcacgcc gagctgcacc
     6541 tgctgatgtc gatgatcacg ccgctcggct ggctggagcg cgtgccgagc tacaaggaac
     6601 agcaggagaa gctgtccgac cgcgacctgg ccacttacgg attcctgggc tatccgctgc
     6661 tgcagagcgc cgacatcctg atctaccggg ccaccctcgt ccccgtgggc gaggaccagg
     6721 tgccgcacat cgagttcacg cgcgagatcg cgcgccgctt caaccatatc tacgggcgcg
     6781 agccgggttt cgaggagaag gccgaggccg cggtcaagaa gcttggcagc aagcgcgcgc
     6841 gcctgtaccg cgagctgcgc gcgcgctacc aggagcaggg cgatcacgag gcgctggaag
     6901 ccggacgcgc gttgctcggg gaagcgcaga acctgagcat gggcgaccag gagcgcctat
     6961 tcggctatct cgagggcggc ggcaagatga tcctgtccga gcccgaagcg ctgctgaccg
     7021 aggcctccaa gatgccgggg ctggatggcc agaagatgtc gaagtcctac aacaacacca
     7081 tcggcctgcg cgaggacgcg gacagcgtca cgcgcaagat ccgcacgatg cccaccgacc
     7141 cggcgcgcgt gcgtcgcagc gacccgggcg agcccgagaa gtgcccggtg tggcagtttc
     7201 acctggtgta ctccgacgag ccgacccgtg agtgggtgca gaagggctgc cgcagcgcgg
     7261 gcatcggctg cctcgagtgc aagcagccgg tgatcgatgc ggtcctgcgc gagcaggaac
     7321 cgatgcgcga gcgcgcgcag gcttacgtgg acgatcccac gctggtgcgc aatatcatcg
     7381 aggacggctg cgagcgggcg cgcaaggaag cgggcgagac gatgcgcgag gtgcgcgagg
     7441 cgatggggct ggcgcagggc tgaggccggt gcgcccttaa ggcgcgactt aagaaccatt
     7501 ccccgcctct cacttgcttg gctacgctcg ttgtcggagc agtcgttgct tctcccgctg
     7561 ccaatcgcgc tccttctctg cctgtctctt atcgtgttgt ttcttgccct tggcaagccc
     7621 gatctcgagc ttgatccagc caccgcggta gtgcatgtcg agcggcacca gggcatagcc
     7681 ggcgcgctcc accttgccga tcaggcgccg cagctcctcg gcgttgagca gcagcttgcg
     7741 cgtgcgggtg ggatcgggct gcacgtgtgt ggaggcggtc ttgagcggcg tgatgtggca
     7801 gccgatcagg tacggcgcgc cgcccttcag gatcacgtag gcctcgctca gatgcacctg
     7861 cccggcgcgg atcgccttga cctcccagcc ctcgagcatg atgccggcct cgtacttctc
     7921 ctcgatgaag tactcgtggt aggccttgcg gttctgggcg atgctcatgg cgggaggctc
     7981 gacgtgcgcg ccgaattgta tcagggcggt ccccgcctcc agcacggaca ccgcgatcgg
     8041 tcgccggacg gcgaaggtgg ccggctgcgc gccggtgcga gcaatgcggg ctagaatccg
     8101 ccgccatggc cctggtccag cgatccgtgc tggtcggcta cgccgcggaa aagatgtacg
     8161 cgctggtcga ccaggtcgag cattaccccg agttcctgcc gtggtgcggg cgaacgcacg
     8221 tggactaccg cgacggcgcg ctgacgcgcg cctcgatcct gatcgacttt cgcggcatca
     8281 agcaggcgtt ccagaccgag aaccgcaccg agccgccgca tctgatcgag atcaagctgg
     8341 tgagcgggcc gttccgcacg ctggacggca cctggcgctt ccagcccctg gcggtggacg
     8401 cttgccgggt ggatttccgc ctgcactacg agttctccag caagatgctc gagcgcctgg
     8461 tggggccggt gttccactac atcgccagca gcttcatcga cgcgttcgtg aagcgcgccg
     8521 agcgcctcta cggcaagggt tgaccctggg cgagacgctc accgtcgaag tcgtgttcgc
     8581 gctgccgctg gagcaggact gcgcggtggt ggaactgccc gatggcgcca gcgtgcgcga
     8641 tgcgattgcg cggtcgggcg tgaccgcgcg gcatccgatc atcgatctcg gcgcgtgccg
     8701 ggtggggatc tgggggaggc ccgctggcct ggacgatcgg gtgcgaaacg gcgaccgcgt
     8761 ggagatctac cggccgctgc aggcggaccc gaaggaatcg cgacgcacgc gcgcgaagcg
     8821 cacgagaagc taggactgtt ccccgctgcc gccgatcact tgcaccagga atcggcgact
     8881 ttttgcgcct cgatcagctc ggcggcgatc tcgttgtcgc cgagatagat ctgctcgccg
     8941 ctggggctcg ccttggtgat gcgcccgccg gtctgcagca ccgcgacgcg cgccttcgcc
     9001 gcctcgcagt tgcgctgctt ctcggtcgcg gcctgggcct cctgctggcg cttggcctcg
     9061 gcttccgccg cttcggtgcg gcgcttgcgg aactccatgt ccagctcggc cgctgtcttg
     9121 gccttggtcg ccggcgccgc ctgaccctcg ggggcggccg gtgtcgtcgc agcgccttgc
     9181 gcacggactt tcggctggat cgtctgctgc tgcctggcgt tggccggcgg tggctggtcg
     9241 gagtagtgca ccttgccggt ctcgtcgacc cacttgtaca tctcggcggc ccaggccggc
     9301 agcgccaggg ccagcagggc cagcgtcgcg atccacttca tatccgctcc tcttcaggga
     9361 aagcgcgggt aatcgagctg ctcgatgccc aatcgcgcct gctcggcggc cgcacggctc
     9421 gcctgcacgg cggcggcatg atcgccccgc gcgagcgctg cctcggcttc tgcgagcgcc
     9481 tcctgcgccg tggtccacaa cgcccgcttc tcggcggcgg cctgcacggc ttgctgcgcc
     9541 cgcaacacct cgcggcgcgc ctgctcggca ccaggatcat tgaccccggc gctcccaggc
     9601 ttggcatctc ctgcgtgcac gctcgctgcg gccagcagcg caccgcatgc gaaagcaaca
     9661 atcgttccct tgtcccacat aagtgcttgt tccgtcagca ggcgcgggcg cctccggcga
     9721 ggccgggccg cgaccgctgc aggcccggcg tataatagat tctttgcgaa ggatcatcag
     9781 catgcgagtg gttgaacggg cgctcacgtt tgacgatgta tccatccttc cgtcccattc
     9841 catcgttctg ccgcgcgacg tcagcctgaa gacccggttg acccgggcga tcgagctgaa
     9901 cctgccgctg gtgtccgccg ccatggacac cgtcaccgag gcgcgcctgg ccatcgccat
     9961 ggcgcaggaa ggcggcgtcg gcatcgtgca caagaacatg gccccgcgcg cccaggccgc
    10021 agaagtggcc aaggtcaagc gctacgaaag cggggtggtg aaagacccga tcaccatcgc
    10081 gccggacatg agcgtgcgcg aggttctgag cctgatccgc gcccacaaga tctccgggct
    10141 cccggtggtg gagggcaagc gcgtggtcgg catcgtcacc aaccgcgact tgcgcttcga
    10201 gaccaatctg gaccagcgcg tgcgcgacat catgaccccg cgcgagcgcc tggtgacggt
    10261 gcgcgagggg ggcagcctgg aggaggccca ggcgctgatg cacaagcacc ggctggagcg
    10321 ggtgctggtg gtcaacgcca atttcgagct gcgcgggctg atcacggtca aggacatttt
    10381 gaaggcgagc gagtacccga acgcctgcaa ggacgagctg ggcaggttgc gggtgggcgc
    10441 cgccatcggc gtgggcgaag gcaccgaaga gcgcgccgag gcgctggtcg aggccggcgc
    10501 cgacgtgctg gtggtggaca ccgcgcacgg gcactccgag ggcgtgctcg agcgcgtgcg
    10561 ctgggccagg cgccagtttc ccaggatgca gatcgtggcc ggcaacatcg ccaccgcggc
    10621 ggcggccaag gcgctggtgg acgccggtgc ggacgcggtc aaggtcggca tcggccccgg
    10681 atcgatctgc accacgcgca tcgtcgccgg cgtcggcgtg ccgcagatca ccgccatcgc
    10741 caatgtcgcg ctggccctga agacgaccga cgtcccgttg atcgccgacg gcggcatccg
    10801 ctactcgggc gacgtcgcga aggcgatcgc cgcgggcgcg gatgcggtga tgctgggcgg
    10861 gctgttcgcc ggcacggaag aggcaccggg cgagatcgag ctgttccagg ggcgctccta
    10921 caagtcctac cgcggcatgg gttcgctggc cgcgatgacg cagggctcga gcgaccgcta
    10981 tttccaggac gccgccgaca gcgtggaagc cctgaagctg gtgcccgaag gcgtggaagg
    11041 gcgcgtgccc tacaagggca gcctgatcca ggtgatcgag cagctcatgg gcggattgcg
    11101 ctcgagcatg
//
LOCUS       DASPGE010000203         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_407390, whole genome shotgun sequence.
ACCESSION   DASPGE010000203 DASPGE010000000
VERSION     DASPGE010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407390"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VD791_06555"
     CDS             complement(<1..259)
                     /locus_tag="VD791_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HXV07672.1"
                     /translation="MPALESAVLVLADGTVFRGRGVGAPGLAAGEVVFNTAMTGYQEI
                     LTDPSYAGQIVTLTYPHIGNTGVNPEDEESHKVFAAGLVVRD"
     gene            complement(346..1086)
                     /gene="dapB"
                     /locus_tag="VD791_06560"
     CDS             complement(346..1086)
                     /gene="dapB"
                     /locus_tag="VD791_06560"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HXV07673.1"
                     /translation="MGRALVEAVRAERDLQLAAALDVAGSPAIGQGAGPLRIGSDLGA
                     VAGADVLIDFTRPAGTLAHLDACVAHGIALVIGTTGFNDAQKLRIGAGARRIPIAMSP
                     NFAIGVNVVFRLAQTAAGALGDAYDVEIVEAHHRHKVDAPSGTALRLGELVAGALGRN
                     LGKVAVHGRQGDTGERPAGAIGFHAIRGGDIVGEHTVMFAGQGERVEITVRSQSRMTY
                     AAGALRAAKWLQGRAAGLYDMSDVLGLK"
     gene            complement(1167..1454)
                     /locus_tag="VD791_06565"
                     /pseudo
     CDS             complement(1167..1454)
                     /locus_tag="VD791_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312138.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
     gene            1474..1911
                     /gene="fur"
                     /locus_tag="VD791_06570"
     CDS             1474..1911
                     /gene="fur"
                     /locus_tag="VD791_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HXV07674.1"
                     /translation="MSSAQSLKDIGLKATLPRRKVLELFESSKVRHLSAEDVYRSLLA
                     EGIDIGLATVYRVLTQFEQAGLLSRQHFETGKAVFELNQGGHHDHLVCLQCGRVEEFY
                     DAEIEQRQSEVARKRGFELHGHSLALYADCTKQACPHRKDLQP"
     gene            complement(1912..>2156)
                     /locus_tag="VD791_06575"
     CDS             complement(1912..>2156)
                     /locus_tag="VD791_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HXV07675.1"
                     /translation="SLRRLGRERQVLCVTHLPQVAAQGDEQWSVRRTSQRGGRVSSGV
                     QRLDRAARIEELARMLGGAEITATTRKHAAELLDAG"
BASE COUNT          336 a          784 c          727 g          309 t
ORIGIN      
        1 cgtcgcgcac cacgagaccg gccgcgaaca ccttgtgcga ctcctcgtct tccgggttca
       61 cgccggtgtt gccgatgtgc ggataggtca gggtcacgat ctgccctgcg taggacggat
      121 cggtgaggat ctcctgatac cccgtcatgg cggtgttgaa caccacttcg ccggccgcca
      181 ggccgggtgc gcccacgccg cgcccccgaa acaccgtccc gtcagcgagg acgaggacgg
      241 cggattcgag cgcaggcaaa gcgcaactcc cggaagtgga tgtgaaaaag cggaagaggc
      301 tcgcgcccct cccgctcgtc agagcgccga attataccgc ggcggctact tcaggccaag
      361 cacgtcggac atgtcataga gacccgccgc gcgaccctgc agccacttcg ccgcgcgcaa
      421 cgcgcccgcg gcgtaggtca tgcggctctg cgagcgcacg gtgatctcca cgcgctcgcc
      481 ctgtcccgcg aacatcaccg tgtgctcgcc gacgatatcg cccccgcgga tcgcgtggaa
      541 accgatcgcc ccggcgggtc gctcgccggt gtcgccctgc cgtccgtgca cggccacctt
      601 gccaaggttg cggccgagcg cgcccgcaac cagttcgccg agcctgagcg ccgtccccga
      661 aggggcatcg accttgtgcc ggtgatgcgc ctcgacgatc tccacgtcgt aggcgtctcc
      721 gagcgcgccc gcggccgtct gcgccagccg gaacacgacg ttgacgccga tggcgaagtt
      781 cggcgacatg gcgatcggga tgcgccgcgc gcctgcgccg atgcgcagct tctgcgcgtc
      841 gttgaagccg gtcgtgccga tcaccagcgc aatgccgtgc gctacgcagg cgtcgaggtg
      901 cgcgagggta cccgccgggc gcgtgaagtc gatcagcacg tccgcacccg caacggcacc
      961 caggtcggaa ccgatgcgca gcgggcccgc cccctggccg atggccgggc tgccggcgac
     1021 atccagggcc gccgcgagct gcaggtcgcg ctccgcccgc accgcctcga cgagcgcgcg
     1081 gcccatgcgc ccccctgcgc cggcgatcgc cactctcact ggccggggtc cggatcccgc
     1141 gcggcggcga cgacgtcgcc ctccacgcgc gtgagcttgt cctcctcgaa gaacaccgcc
     1201 agccgccgct tctgcagcgt ctgtccgggg cggtcgagca ggtacacgta gtcccagcgg
     1261 tcggcgtgga agatgtcggt gaccagcggc gtgccgagga ggaagcgcac ctgctcccgg
     1321 gtcatgccgc ggcgcagctg cgccaccatc tcctgcgaca cgaagttgcc ctgctggatg
     1381 tccatgcgat agggcgccag cgggccgcag ccggcggcga gcagcgcgag ggagacaagg
     1441 atcgtgcggc gcatcgtcat ataatgataa tcgatgagtt ccgcgcagag cctgaaggac
     1501 atcggactca aggccacctt gccccggcgg aaggtgctgg aactcttcga gagcagcaag
     1561 gtgaggcacc tctccgcgga ggacgtctac cgcagcctgc tcgccgaggg aatcgatatc
     1621 ggtctggcca cggtctatcg cgtgctgacg cagttcgagc aggcggggct gctgtcgcgc
     1681 cagcacttcg agaccggcaa ggcggtgttc gagctcaacc agggcgggca ccacgatcac
     1741 ctggtctgcc tgcagtgcgg ccgcgtcgag gagttctacg acgccgagat cgagcagcgc
     1801 cagagcgagg tggcgcgcaa gcgcggcttc gagctgcacg ggcattcgct ggcgctgtac
     1861 gcggactgca ccaagcaggc ctgcccgcac cgcaaggacc tgcagcccta gctagcccgc
     1921 gtcgagcagc tccgcggcgt gcttgcgcgt ggtggcggta atctcggcgc cgcccagcat
     1981 ccgtgccagt tcctcgatgc gcgccgcacg gtccaggcgc tgcaccccgc tcgacacccg
     2041 gccaccgcgc tggctcgtgc gcctcacgct ccattgctcg tcgccctgcg ccgccacttg
     2101 cggcaggtgc gtgacgcaca ggacctggcg ctcgcggccc agccggcgca gcgacc
//
LOCUS       DASPGE010000204         4994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_291742, whole genome shotgun sequence.
ACCESSION   DASPGE010000204 DASPGE010000000
VERSION     DASPGE010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291742"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..700)
                     /locus_tag="VD791_06580"
     CDS             complement(92..700)
                     /locus_tag="VD791_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HXV07676.1"
                     /translation="MSEILSPPLTPEWLLQRLCAPLDAAEPGFLRYVHPPEQSVTPAA
                     VLVPLINRSAGVTVMLTQRTTHLNDHAGQISFPGGRVEEGDADRIQTALRETEEETGL
                     DRAAVQVVGSLPDYDIPTGFRVTPVVGWIEPPVRFDPDPFEVAEVFEVPLGHFLDPAN
                     HQRHSDLVNGRRRNYYAMPYQQRHIWGATAGMLFSLYRALVP"
     gene            874..1119
                     /locus_tag="VD791_06585"
     CDS             874..1119
                     /locus_tag="VD791_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07677.1"
                     /translation="MRLRTLFAALALVAASATAFAAHCPMDMKAIDEALAKNPSLSAE
                     QLADVKKYRAEGEALHKAGKHKESVETLGKARKILGI"
     gene            complement(1148..1855)
                     /locus_tag="VD791_06590"
     CDS             complement(1148..1855)
                     /locus_tag="VD791_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV07678.1"
                     /translation="MARPRIAVVTGGNKGIGFEICRQLAARGINVVLTARSRRRGTLA
                     ARKLQGEGLNVIFHPLDVTVPAEVQALARYLDAAHGRCDVLVNNAGVALDRYSMSVLA
                     TPLRLFRDTLDTNFYGPLRLCQALMPMMLRKRYGRVVNLSSGLGQLEDMEDGSAAYRV
                     SKTALNALTRMAAAAAGNRNVLVNSMCPGWVRTDMGGRNAARSVEKGAETAVWLATLP
                     EGGPSGGFFRDRRPMPW"
     gene            complement(1903..2427)
                     /locus_tag="VD791_06595"
     CDS             complement(1903..2427)
                     /locus_tag="VD791_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6584 family protein"
                     /protein_id="HXV07679.1"
                     /translation="MSMYAETLKLVAHLAQRRPAPMLPSRLDQLFRHLALPGCQAEDL
                     EEEIWQLWMHHPHRRAAQALDRAASDIAAQRFDLAETRLQRLVRACPDFPEAWNKRAT
                     LYYLQERDAESVRDIHRTLELEPRHFGALCGLGEICLSRGDVEAALFVFHAALRVNPH
                     LDGTRDRIEHLMHP"
     gene            complement(2460..4730)
                     /locus_tag="VD791_06600"
     CDS             complement(2460..4730)
                     /locus_tag="VD791_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HXV07680.1"
                     /translation="MESTFRQAALDYHRLPKPGKVAVTPTKDLLNQRDLALAYSPGVA
                     AACEEIVADPAHARDYTARGNLIGVVTNGTAVLGLGAIGPLAAKPVMEGKAVLFKKFA
                     GIDVFDIEIDERDPQRLVDIIASLEPTFGGINLEDIKAPECFAIERKLRARIRIPVFH
                     DDQHGTAIIVGAAVLNGLKVVGKDIRRVQLVCSGAGAAALSCLDLLVHLGLPLDNVTV
                     ADIKGVVYRGRTEEMDEFKARYARDTAARTLGEIIEGADIFLGLSAGGVLKPEMVKRM
                     AQRPLILALANPVPEIMPEEAKAARPDCIIATGRSDYPNQVNNVLCFPFIFRGALDAG
                     ATTITEEMKLAATRAIAELAQAEQSDVVTMAYGEASQAFGPEYLIPRPFDPRLIVKIA
                     PAVAQAAMASGVATRPIADLDGYRQQLTQFVYHSGLIMKPVFEAAKSAPKRVVYCEGE
                     DQRVLQAVQVVLDEGLARPIVIGRPEVVRGRLEKLGSRMQPGRDFELVNPDSDPRFRD
                     YWRLYHDIMQRRGVSVEVAKREVIRRSTLIGALMLRQGEADAMLCGTYGEHATHLAYI
                     GNVIGLKPGVKQFAAMNLLLLQERTLFLCDTYVNPDPSAEQLAEIALLAADEVRRFGL
                     TPKVALLSHSSFGSARTPSAQKMSRVRELLDRAAPQLEVEGEMHGDAALSEEIRMQVF
                     PNSRFKGEANLLVMPTLDAANISFNLLKVVAGEGITVGPILLGAAKTCHVLTPTATVR
                     RLVNMTALAVVEAGAR"
BASE COUNT          760 a         1748 c         1708 g          778 t
ORIGIN      
        1 ccgcttcttt gactcgatct gctgatgcaa tccttgtccg ccgccacatg cggcccgcaa
       61 ccgccctcgg acgcgctcgc cccggatcac ctcacgggac cagcgcccgg tacaggctga
      121 acagcatgcc ggcggtggcc ccccagatgt gccgctgctg gtagggcatc gcgtagtagt
      181 tgcggcgccg gccgttgacc aggtcgctgt ggcgctggtg attggccgga tccaggaaat
      241 ggccgagcgg cacttcgaac acctccgcca cctcgaacgg gtcggggtcg aagcgcaccg
      301 gcggttcgat ccatcccacc accggcgtca cgcggaaacc ggtagggatg tcgtagtcgg
      361 gtaagctgcc gacgacctgg accgcagccc ggtcgagacc ggtctcttcc tcggtctcgc
      421 gcagcgcggt ctggatgcgg tcggcatcgc cctcctccac gcggccgccc gggaagctga
      481 tctggccggc atggtcgttc agatgcgtgg tgcgctgcgt gagcatcacg gtgacgccgg
      541 ccgagcggtt gatcagcggt accaggaccg ccgccggggt gacgctctgc tcgggtggat
      601 gcacgtagcg caggaacccc ggctccgcgg cgtccagcgg ggcacacagc cgctgcagga
      661 gccattccgg cgtaagcgga ggggagagaa tctcggacac gggcgggctt caagcaaact
      721 tggttcctgc cggctattct aagccttgcc gcgccggccg tcgccctcgt cccgggtggg
      781 cgcggcggcg ggaaacctta ttgggggcgg cggcatccaa gggggacggg ccgtgcaccg
      841 cacccgcccc atcccaacca gcaggaggac ccgatgagac ttcgtaccct gttcgccgcc
      901 ctcgcgctcg tggcggccag cgccaccgcc ttcgccgcgc attgtccgat ggacatgaag
      961 gcgatcgacg aagccctggc gaagaacccc agcctgagcg ctgagcagtt ggcggacgtc
     1021 aagaagtacc gcgccgaggg cgaagcgctg cacaaggctg gcaagcacaa ggagtcggtc
     1081 gagactctgg gcaaggcccg gaagatcctc gggatctaga cccccggcgg cggcgccacc
     1141 gggccggtca ccaaggcatc ggcctgcggt cgcggaagaa accgccgctc ggcccgccct
     1201 cgggcaacgt ggcgagccac accgcggttt cggcgccctt ctcgacgctg cgcgcagcgt
     1261 tgcgcccacc catgtcggtg cgcacccagc cggggcacat cgagttcacc agcacgttgc
     1321 gattgcccgc ggcggcggcc gccatgcgcg tgagcgcatt gagagcggtc ttggaaacac
     1381 ggtacgctgc gctgccgtcc tccatgtcct cgagctgccc caggccgctg gaaaggttga
     1441 ccacgcgccc atatcgcttg cgcagcatca tcggcatcag cgcctggcac agccgcagcg
     1501 ggccgtagaa gttggtgtcc agcgtgtccc ggaacagccg cagcggagta gcgagaacgc
     1561 tcatgctgta gcggtcgagc gcgacgccgg cgttgttcac cagcacgtcg cagcgcccgt
     1621 gcgcggcgtc cagatagcgg gcgagcgcct gcacctcggc cggcacggtg acgtccagcg
     1681 ggtggaagat cacgttcagg ccctcgcctt gcagcttcct cgcggccagt gtgccgcgcc
     1741 gccggctgcg cgccgtcagc accacgttga tgccgcgcgc ggcgagctgt ctgcagatct
     1801 cgaaaccgat gcctttattg ccgccggtga cgacggcgat gcgtggcctg gccatatgcg
     1861 cccaaggcgc gattcaagag accccctact ctaaatcgcg ccctatgggt gcatcaagtg
     1921 ctcgatcctg tcgcgcgtgc cgtcgaggtg cggattcacg cgcagcgcgg cgtggaatac
     1981 gaacagcgcc gcctccacgt cgccgcggct caggcagatc tcgcccaggc cgcacagggc
     2041 gccgaagtgg cgcggctcca gctccagggt gcgatggatg tcgcgcacgc tctccgcgtc
     2101 gcgctcctgc aggtagtaga gcgtggcacg cttgttccag gcctcgggga aatccgggca
     2161 ggcgcgcacc aggcgctgca ggcgcgtctc ggccagatcg aagcgctgcg cggcgatgtc
     2221 gctggccgcc cggtccagcg cctgcgccgc acggcggtgc ggatggtgca tccacagctg
     2281 ccagatctct tcctcgaggt cctcggcctg gcacccgggc agggccaggt gccggaacag
     2341 ctggtccagg cgcgagggca gcatcggcgc ggggcggcgc tgcgcgaggt gcgcgaccag
     2401 cttcagcgtc tcggcgtaca tgctcatagc gcaagcaaag cggatgccgg agcctctcgc
     2461 taacgcgccc cagcctccac caccgcgagc gccgtcatgt tgaccagacg gcgcaccgtc
     2521 gcggtcggcg tcagcacatg gcaggtcttg gccgcgccga gcaggatcgg gcccaccgtg
     2581 atgccctcgc ccgccaccac cttgagcagg ttgaaggaga tattggcggc gtcgagcgtg
     2641 ggcatgacca gcaggttggc ctcgcccttg aagcgcgagt tggggaacac ctgcatgcgg
     2701 atctcctccg acagcgcggc gtcgccgtgc atctcgcctt cgacctcgag ctgcggcgcg
     2761 gcgcgatcga gcagctcgcg cactcggctc atcttctgcg ccgaaggcgt gcgcgcgctg
     2821 ccgaagctcg aatgcgacag cagcgccacc ttcggcgtca gcccgaagcg ccgcacctcg
     2881 tcggcggcga gcagggcgat ctcggcgagc tgctcggcgc tcgggtcggg attgacgtag
     2941 gtgtcgcaca ggaacagggt gcgctcctgc agcagcagca ggttcatcgc cgcgaactgc
     3001 ttcaccccgg gcttgagccc gatcacgttg ccgatgtagg cgagatgcgt ggcgtgctcg
     3061 ccgtaggtgc cgcacagcat ggcgtcggcc tcgccctggc gcagcatcag cgcgccgatc
     3121 agcgtgctgc ggcggatcac ctcgcgcttg gccacctcga ccgacacgcc tcggcgctgc
     3181 atgatgtcgt ggtacaggcg ccagtagtcg cggaagcgcg gatcgctgtc gggattgacc
     3241 agctcgaagt cgcggcccgg ctgcatgcgc gagccgagct tttccaggcg cccgcgcacc
     3301 acctccggcc ggccgatcac gatcggccgc gccagacctt cgtccagcac cacctgcacc
     3361 gcctgcagca cgcgctggtc ctcgccctcg cagtacacca cgcgcttggg cgcgctcttg
     3421 gcggcttcga acaccggctt catgatcaag ccggagtggt acacgaactg cgtgagctgc
     3481 tggcgatagc cgtcaaggtc ggcaatcggg cgcgtggcca cgccgctcgc catggccgcc
     3541 tgcgccaccg ccggggcgat cttgacgatc aggcgcgggt cgaacgggcg cgggatcagg
     3601 tactcggggc cgaaggcctg gctcgcctcg ccgtaggcca tggtcaccac gtcggactgt
     3661 tcggcctgcg ccagctcggc gatggcgcgg gtggccgcca gcttcatctc ctcggtgatg
     3721 gtggtggccc ccgcatccag cgcgccgcgg aagatgaacg ggaagcacag cacgttgttc
     3781 acctggttgg gatagtccga gcggccggtg gcgatgatgc agtcgggccg cgccgccttg
     3841 gcctcctcgg gcatgatctc cggcaccggg ttggcgagcg ccaggatcag cggacgctgc
     3901 gccatgcgct tgaccatctc gggtttgagg acgccgcccg ccgacaaacc caggaagatg
     3961 tcggcgccct cgatgatctc ccccagcgtg cgcgccgccg tgtccctggc gtagcgcgcc
     4021 ttgaactcgt ccatctcctc ggtgcggccg cggtagacca cgcccttgat gtcggccacg
     4081 gtcacgttgt ccagcggcaa cccgaggtgg accagcagat ccaggcacga cagcgctgcc
     4141 gcgccggctc ccgagcacac cagctggacg cggcgaatat ccttgcccac caccttgagg
     4201 ccgttcagca ccgccgcgcc gacgatgatc gcggtgccgt gctggtcgtc gtggaacacc
     4261 gggatgcgta tgcgggcacg cagcttgcgc tcgatcgcaa agcactccgg cgccttgatg
     4321 tcctccaggt tgatgccgcc gaaagtgggc tcgaggctgg cgatgatgtc caccaggcgc
     4381 tgcgggtcgc gctcgtcgat ctcgatgtcg aacacgtcga tgccggcgaa cttcttgaac
     4441 agcacggcct tgccctccat caccggcttg gccgcgagcg gcccgatcgc gcccagcccg
     4501 agcacggcgg tgccgttggt gacgaccccg atcaggttgc cgcgcgcggt gtagtcgcgt
     4561 gcgtgcgccg ggtcggccac gatctcctcg caggcggcgg ccacgccggg cgagtaggcg
     4621 agcgcgaggt cgcgctgatt caacaggtcc ttggtgggag tgaccgcgac cttgcccggc
     4681 ttgggcaggc ggtgatagtc gagggcggcc tggcgaaacg tggattccat cggaagctct
     4741 ggcgctgtgt gcgatgcgaa cattatatgc gggcatcgcg catgctctgc gcacgcggcg
     4801 catgcgcgat cggcaaggcc actctgctac actcgcccga tcccaccacc cggagatgct
     4861 cttgttcctg caccggctga tcgcgccgcg gctgcgcgcc gcgctggccc tggcagcggc
     4921 tgcgcccctg ctcgccttcg cgcaggccgg cagcacgccg ctgctctacg aagtgcgctc
     4981 gcccagcaac accg
//
LOCUS       DASPGE010000205         4686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_407640, whole genome shotgun sequence.
ACCESSION   DASPGE010000205 DASPGE010000000
VERSION     DASPGE010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407640"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="VD791_06605"
     CDS             <1..1112
                     /locus_tag="VD791_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HXV07681.1"
                     /translation="PEARRYFMTIPEAAQLVLLEMSEVALYELERELRARMAREQMSG
                     TELIAVLGDAHNKSRMREVMLAFGVQTIYHAAAYKHVPIVERNVIEGVSNNVFSTWYT
                     AEAARETGAETFVLVSTDKAVNPTNVMGAAKRLAEIVLQSMQVQTTGTRFCVVRFGNV
                     LGSSGSVVPLFQEQIRAGGPVTVTHPEARRYFMTIPEAAQLVLQAGSMATGGDVFVLD
                     MGQLVRIENLARRMIGLLGLTVRDETTPEGDIEIKYTGLRPGEKLYEELLISDNVTKT
                     GHPMIMRALEPSPGWDEVGRLLAELNVAVNRLDCVQVLEILQRGVAEYHRQPVMHDVV
                     WNNRPPEVAVPEVVETRKVAVLAEHRGQKGSAGTT"
     gene            1559..2641
                     /locus_tag="VD791_06610"
     CDS             1559..2641
                     /locus_tag="VD791_06610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV07682.1"
                     /translation="MSHKLARTLHERGCRVTLLASEAPRPSYLDMSVVRARAPFGSVL
                     RPILNLPTLLQQALALRADLYLACNPDTLVLAIALAALGERVIYDTAEDFAQRPYIHP
                     AVPRLLAPLVAKSLEAGERLLTRLAVGVCVTQEPIRARRGNCLLLANAPLTRGPVVAE
                     AERIFASIARPACDLCLVYAGLLTELRGLWRMLDLVARLNDHLQVRLVLAGEFHPAGL
                     LGAAERHPGWKFVHYQGQVSHAEALAWIRCSDLGLALLERVADYPTSSITKIYEYMQY
                     GTPFVASDFQAWRASINDVAAGLFVEPADLDGAVAGILALARDPGRLERMRDVGRNFI
                     HEHFNWHVVSKPFVELVDSAIAGRGR"
     gene            2654..4444
                     /locus_tag="VD791_06615"
     CDS             2654..4444
                     /locus_tag="VD791_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV07683.1"
                     /translation="MSTRESAGVLGFSREVFAFAGAGGLATLALMTLVAVSEGAGIVL
                     LVPLLALVGIAPQQQLPAWTHGLLDGDAGPGGLYLVLAVFLGLLAARQLLLLRQNERV
                     ARLRFRFVNHIRRRVLEALMQVQWRYLSRGMLQRQAQVLTLDVNRIGEACELLFRLGA
                     AALFLAMQVTIAAVLAPGFTALALGSMILTQWLLRRRLVLASHRGDALTQRHNALFVV
                     LSNLKGMLRITRMTGASQRLLDTFERETGALAEQAVAYARDHEWGRILVHLAGAAVLC
                     VALVAGVEVFALDAVEILLLVVIFARLIPQLAGAQQMLHRLFHALSAYEHAARSVELL
                     RDRCESAAGAMHPVQPPARVALEDIVAGYDDEAGGPVLRGAALCLGAGEVVALTGDSG
                     SGKSTLADVLAGLLEPLSGRIVLDGIPLDAGRLPAWRRAVGYVEQDAALLTGTLLENL
                     TFGTGEQDPRRIARALGASLSAEFVAALPDGLATHVGEHGSRLSGGQRQRVALARELL
                     REPLLLVLDEATSGLDAETEARVFEQLRRHYPSLTILVITHRTSAIGTMDRLLEMRGG
                     QVHEAPRVPPAVIRERRAAGPAAMLATTAP"
     gene            4441..>4686
                     /locus_tag="VD791_06620"
     CDS             4441..>4686
                     /locus_tag="VD791_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07684.1"
                     /translation="MIRIAQLPLEAMRFSRASPAAALALRPADLEPLDFAILSTVAYR
                     DQFGFAPTLDEIHRYLHWIACDRTEVAQALSDGRLVGR"
BASE COUNT          710 a         1531 c         1642 g          803 t
ORIGIN      
        1 acccggaagc gcggcgatac ttcatgacga ttccggaggc cgcgcagctc gtgctgctgg
       61 aaatgtccga ggtggcgctc tacgaactgg agcgcgagtt gcgtgccagg atggcgcgcg
      121 agcagatgtc cggcaccgag ctgattgcgg tactgggcga cgcgcacaac aagtcgcgga
      181 tgcgggaagt gatgctggcg ttcggcgtac agacgatcta ccacgcggcg gcatacaagc
      241 acgtcccaat cgtcgagaga aacgtcatcg agggcgtgag caacaacgtc ttcagcacct
      301 ggtatacggc cgaggctgcg cgcgaaacag gcgccgagac gttcgtcctg gtgtcgaccg
      361 acaaggcagt gaaccccacg aatgtcatgg gcgccgcgaa gcgcctggcg gaaatcgtgc
      421 tgcaatccat gcaggtgcag acgacgggca cccgattctg cgtggtgcgc ttcggcaacg
      481 tgctcgggtc atcgggatcg gtcgtgccct tgtttcagga gcagatccgt gcgggtggcc
      541 cggtcaccgt gacccacccg gaagcgcggc gatacttcat gacgattccg gaggccgcgc
      601 agctcgtgct gcaggccgga tcgatggcga cgggcggcga cgtgttcgtc ctcgacatgg
      661 gccagctcgt gcgcatcgag aacctcgcga ggcgcatgat cgggttgctg ggtctgacgg
      721 tccgcgacga gacgactcct gagggtgaca tcgagatcaa gtacacggga ctgcgtccgg
      781 gcgagaagct ctacgaagaa ctgctgatca gcgacaacgt gacgaaaacc ggtcatccga
      841 tgatcatgcg tgcgctcgag cccagccccg gctgggatga ggtcggccgg ctgctggccg
      901 agctcaatgt cgccgtcaat cgcctggact gcgtccaggt gctggaaatc ctgcagcgcg
      961 gcgtggccga gtatcaccgg cagcctgtca tgcacgatgt cgtgtggaac aatcgtccgc
     1021 cagaagtcgc agtgccggaa gttgtggaga cccgcaaggt tgcggtgctg gcggagcatc
     1081 gaggccagaa ggggtctgcg ggcaccactt aggaggcggc aggcttcctt cacgacgggc
     1141 gccaggagcg gggtcgcgcc cgtgctccag gagcaaattc gggcggcccg ccagcgatcg
     1201 cccgtaccgc ggccccggtc cgcctggcct gggaaagcct aataatatca atacattata
     1261 ttttcaactc tggtggatgt tgtccctttt tgaggtcgat cgggaactgg catacagtgc
     1321 ccggacaaga acttggcagt tgaccagtcg aacgaccggc tgcttgaaca atacgggatg
     1381 cccttagcgg acccacttgc gcgaaacaga ccgcacgtca gaggtgtttc gacagcttcc
     1441 ggcaggcgcg ccgccggaat cgcggcttcc atggcttggg caaccgccca cgaagctcaa
     1501 cggcagcggg cgacatgtcg cggtgctcat gccgcgctgc cgtcatgacg acatccggat
     1561 gtcgcacaag ctcgcccgga cactccacga gcgcggctgc agggtaacgc tgctggcctc
     1621 cgaggctccg cggccgagtt atctcgacat gagtgtcgtc agggcgcgcg cgccattcgg
     1681 ctcggtgctg cgtccgattc tcaatctgcc gacgctgttg cagcaggcgc tcgcgctgcg
     1741 cgcggacctg tacctcgcct gcaatcccga cacgctggtc ctggcgatcg cgctcgccgc
     1801 cctgggtgag cgcgtcattt acgataccgc cgaggatttt gcgcagcgcc cctacatcca
     1861 tcccgcagtc ccgcggttgc tggcgccgct ggttgcgaag tccctggagg ccggcgaaag
     1921 gctgttgacg cgactggcgg tcggggtgtg cgtgacacag gagccgatcc gcgcgcgacg
     1981 cgggaactgc ctgctgcttg cgaacgcacc attgacccgg gggccggtcg tcgccgaggc
     2041 cgagcgcatc tttgcgtcga tcgcgcggcc cgcctgtgac ctctgcctcg tctatgcggg
     2101 actcctgacg gaattgcgcg gcctgtggag gatgctggac ctcgtcgcgc ggctgaatga
     2161 ccaccttcag gtgcgcctgg tgctcgccgg cgagtttcac cccgctggcc tgctgggcgc
     2221 cgccgagcgc cacccgggtt ggaagttcgt ccactaccag gggcaggtgt cccacgcgga
     2281 agcactcgcg tggatccggt gctcggacct cgggctggcg ctgctcgagc gcgtggcgga
     2341 ctatccgacg tcgagcatca cgaagatcta cgagtacatg cagtacggga cgccattcgt
     2401 cgcgagcgac ttccaggcct ggcgggccag catcaatgac gtcgccgccg gactgttcgt
     2461 cgagccggcg gacctggatg gcgcggtggc cggcatcctc gccctggcgc gtgatcccgg
     2521 ccgcctggag agaatgcggg acgtcggccg gaatttcatc cacgagcatt tcaactggca
     2581 cgtggtttcg aagccgttcg tcgagctcgt tgacagcgcc attgcgggcc gcgggcgctg
     2641 acgggccgcc ggcgtgagca cgcgagagtc cgctggtgtg ctcggcttct cacgcgaggt
     2701 cttcgccttc gcgggcgccg ggggactggc gaccctggcg ctgatgacgc tggtggccgt
     2761 ttctgagggc gccggaatcg tcctgctggt gccgctgctc gcgctggtgg gaatagcgcc
     2821 gcaacaacag ttgcccgcct ggacgcacgg actgctcgac ggggacgcgg gccccggggg
     2881 cctgtacctg gtgctcgcgg tcttcctggg gctgctggcc gcacgccagc tgctgctgct
     2941 gcggcagaac gagcgcgtgg cgcgcctgcg gttccgcttc gtcaaccaca tccggcgtcg
     3001 cgtgctggaa gcgttgatgc aggtgcaatg gcgctatctt tcgcgcggga tgctgcagcg
     3061 ccaggcgcag gtgctgacgc tcgacgtcaa tcgcatcggc gaggcctgcg aactgctgtt
     3121 ccgcctcggc gccgcggcgc tgttcctcgc catgcaggtg accattgccg ccgtgctggc
     3181 gccgggcttc actgccctgg cgctcggctc gatgatcctg acgcagtggc tgctgcgccg
     3241 gcgcctggtg ctcgcctccc atcgcggtga tgcgctgacc cagcgccaca atgcgctgtt
     3301 cgtggtgctg tcaaacctga agggcatgct gcggatcacg cgcatgaccg gcgcatccca
     3361 gcgcctgctg gacacgttcg agcgcgagac cggcgccctg gccgagcagg ccgtggccta
     3421 tgcgcgcgac cacgagtggg gccggatcct ggtgcacctg gcgggagccg ccgtgctgtg
     3481 cgtggccctg gtcgccggcg tggaagtgtt tgcgctcgac gcggtcgaga tcctgttgct
     3541 ggtggtgatc ttcgcgcgcc tgatcccgca gctggcgggc gcgcagcaga tgctgcaccg
     3601 cctgttccac gcgctgtcgg cctacgaaca tgcggccagg tcggtcgagc tgctgcgcga
     3661 ccgctgcgaa tccgccgcgg gcgcgatgca cccggtccag ccgccggcaa gagtcgcgct
     3721 cgaggacatc gtcgccgggt acgacgacga ggccggcggg cctgtcctgc gtggtgcggc
     3781 gctgtgtctc ggcgccgggg aagtcgtcgc cctgaccggc gactccggca gcggcaagag
     3841 cacgctcgcc gacgtgctgg ctggcctgct cgagcccttg agcgggcgta tcgtcctcga
     3901 cggaattccg ctcgacgccg ggaggctgcc ggcctggcgc cgtgcggtcg gctacgtcga
     3961 gcaggatgcc gcgctcctga ccggcacgct gctggagaac ctgacctttg gtaccggcga
     4021 gcaggatccc cgcaggatcg cgcgcgcact cggcgccagc ctgtctgcgg aattcgtcgc
     4081 cgcgcttccg gacgggctcg ctacgcacgt cggcgagcac ggcagccgcc tgtccggcgg
     4141 gcaacgccag cgcgtcgcat tggcgcgcga actgctgcgc gagccgctgc tgctggtgct
     4201 cgacgaggcc accagtggcc tcgatgccga gacggaagcg cgcgtgttcg aacagctgcg
     4261 gcgtcattac ccatcgctga cgatcctcgt catcacgcac cgcacttcgg ccatcgggac
     4321 aatggacagg ctgctcgaga tgcgcggcgg ccaggtccac gaggcgccac gggtgccgcc
     4381 ggcggtgatc cgtgagcgac gggctgccgg gccggcggcg atgctggcga cgactgcgcc
     4441 atgatcagga ttgcgcagct gccgctggag gcgatgcgat tttcgcgcgc ttcgcccgcg
     4501 gctgcgctgg ccctgcgacc ggcggatctc gagccgctgg atttcgcgat cctctcgacc
     4561 gtcgcctacc gcgaccagtt cggttttgcg ccgaccctgg acgagatcca ccgctacctg
     4621 cattggatcg cctgcgatcg caccgaggtg gcgcaagcgc tcagcgacgg gcggctggtc
     4681 gggcgt
//
LOCUS       DASPGE010000206         3694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_876289, whole genome shotgun sequence.
ACCESSION   DASPGE010000206 DASPGE010000000
VERSION     DASPGE010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876289"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..404)
                     /locus_tag="VD791_06625"
     CDS             complement(255..404)
                     /locus_tag="VD791_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07685.1"
                     /translation="MCFEFEYLYWAKLEEEARQRKEQEQRRRQAAQPPQEPAPAPQQQ
                     DPVPA"
     gene            complement(538..1710)
                     /locus_tag="VD791_06630"
     CDS             complement(538..1710)
                     /locus_tag="VD791_06630"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HXV07686.1"
                     /translation="MKDFRLEALMEITSRPEIVFVKGEGGWLWDHTGKRYLDFVQGWA
                     VNCLGHSPRELVEALTRQARLLITPSPAFYNEPSIRLADALTQRSCFDRVFFASIGAE
                     ANEGGIKLARKYGALHRGGAYEIITFDGAFHGRTLAAMSASGKKQFEALFEPKVPGFP
                     KAILNDLDSVERLIGERTVAVMLEPIQGEAGVLTASDAFLRALRELTVRRGLLLIVDE
                     VQTGMGRTGKLFAYEHAGVEPDMMTLGKGIGGGVPLAALLAKEEVCCFEHGDQGGTYN
                     GNPLMTAAGLAVLETVSAAAFLRAVESMGAHLAGELERLSARRGLGSVRGRGLLLALD
                     LGRPNGGDVVTRAREAGLLINSPRPDSLRFMPALNVTREEIGQMIAMLDRALAQGQ"
     gene            complement(1719..2333)
                     /locus_tag="VD791_06635"
     CDS             complement(1719..2333)
                     /locus_tag="VD791_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXV07687.1"
                     /translation="MTRPVAAMFLSLGLALLAPAASANVAPGQAAPDFTLTDIQGKPQ
                     KLSAFRGKYVVLEWFNSECPFVQKHYESGNMQSLQRRYGDKGVVWLTINSTNPQSSNY
                     RDPARSREIVRDWKINAAALMLDTEGTVGQTYGARTTPQMWVIDPAGKVIFAGGIDDK
                     ATFRTADVKDAKNFVAAALDESMAGRAVTTSAATPYGCSVKYKY"
     gene            complement(2330..>3694)
                     /locus_tag="VD791_06640"
     CDS             complement(2330..>3694)
                     /locus_tag="VD791_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXV07688.1"
                     /translation="VLVAQSGFGSARALEIDVPIEIAAPARSDGTLVALLTTLVLAFV
                     GGVLLNLMPCVFPVLAIKVLGVVQQTHGRAARLRSHGLLFTLGVLLSFWAIAGLLLAL
                     RAQGAALGWGYQLQSPAIVAALALLFFALGLNLSGVFRLGGTVQSLAGSMRARGAHAD
                     ALLTGMLATAVASPCTAPFMGAALGFALVQPALDAMLVFSALALGMAAPYVLLCFVPQ
                     LIRRLPRPGPWMETLRQLLAFPLYATVVWLVWVLGQQVGVDGVARLLAGLVLVAAALW
                     AVGRWGHAAPNARTAARAIGLVLILTGLAVAWPDRRLQQTVQAAQSAWQPWSEEAVRS
                     ALAQGRPVFVDFTAAWCVTCQVNKRLVLSTQAVERRFVELGIARFRADWTNRNPAITA
                     ALARMKRSGVPVYALYSPPGGPPVLLPEVLTRRLVLEALERAASRPAQASAMITSHRS
                     KETP"
BASE COUNT          573 a         1304 c         1265 g          552 t
ORIGIN      
        1 gcgcgaacgg cagcagtgcc agcgccaagg cggcggtgag tttcttcatt gtccggtttc
       61 ctcctcttga tcggggtgag acgcatgctg cccgcacccc gcggcggcgt caatttcctc
      121 cgggcggatg cccgccccgc cgcgcgtctg ctggcccggc tctgatcgcc ggcggaaaaa
      181 agaaagcccc gcatagcggg gctttttctt tcgagccccg cgcggcggag ctcttcttca
      241 gtgacaggag tcgatcaggc gggcaccggg tcctgctgct gcggtgcggg agccggctcc
      301 tgcgggggct gcgcggcctg ccggcggcgc tgctcctgtt ccttgcgctg gcgcgcttct
      361 tcttccagct tggcccaata cagatactcg aactcgaaac acatggcgaa ccctccttgc
      421 ggatgggggc acatcggctc gtgttcttct tgtggcgagg cccatgctac gccggtgaca
      481 cggcgtccgc aatgcgtggg tgtgctgcac cgcagcgccg ggtgtgcgct ccgggcgcta
      541 ctgcccttgc gcgagggccc ggtccagcat ggcgatcatc tggccgatct cctcgcgtgt
      601 cacgttgagc gcgggcatga agcgcagcga gtcgggccgc ggcgagttga tcagcagccc
      661 cgcctcgcgc gcgcgcgtca cgacgtcccc gccgttcggc cgcccgaggt cgagcgcgag
      721 cagcagcccg cggccgcgca cgcttcccag gccgcggcgc gccgagaggc gttccagttc
      781 gccggcgagg tgtgcgccca tcgactccac cgcgcgcagg aaggccgctg cgctgaccgt
      841 ttccagcacc gcgaggccgg ccgcggtcat cagcggattg ccgttgtatg tgccgccctg
      901 gtcgccgtgc tcgaagcagc acacctcttc ctttgcgagc agtgccgcca gggggacgcc
      961 gccgccgatg cctttaccca gcgtcatcat gtccggctcg acgccggcgt gctcgtaagc
     1021 gaacagcttg ccggtgcggc ccatgccggt ctgcacctcg tccacgatca gcaacaggcc
     1081 gcggcgcacc gtcagctcgc gcagcgcgcg caggaacgca tcgctcgccg tcagcacgcc
     1141 ggcttcgccc tggatcggct ccagcatcac ggccacggtg cgttcgccga tcaggcgctc
     1201 gaccgagtcc aggtcgttca ggatcgcctt cgggaagccc ggcaccttgg gctcgaacag
     1261 ggcttcgaac tgcttcttgc cggaggccga catggcggcg agcgtgcggc cgtgaaacgc
     1321 cccgtcgaag gtgatgatct cgtaggcgcc gccgcggtgt agcgccccgt acttgcgcgc
     1381 cagcttgatg ccgccctcgt tggcttccgc cccgatgctg gcgaagaaca cgcgatcgaa
     1441 gcagctgcgt tgcgtgaggg cgtcggcgag ccggatgctc ggctcgttgt agaacgccgg
     1501 gctcggggtg atcagcaagc gggcttgacg ggtgagcgcc tcgaccagct cccgcgggga
     1561 atgccccagg caattcaccg cccagccttg cacgaagtcc aggtagcgct tgccggtgtg
     1621 gtcccacagc cagccgccct cgcccttgac gaacacgatc tcgggccggg aggtgatttc
     1681 catcaacgct tcgaggcgaa agtccttcat ggagcgtctc agtacttgta cttgaccgag
     1741 cagccgtacg gcgtggccgc cgatgtggtg accgcgcggc cggccatgct ctcgtccagc
     1801 gcggcggcga cgaagttctt cgcgtccttg acgtcggcgg tgcggaaggt ggccttgtcg
     1861 tcgatgccgc cggcgaagat caccttgccg gcgggatcga tcacccacat ctgcggcgtg
     1921 gtgcgcgcgc cgtaggtctg gcccaccgtg ccctcggtgt ccagcatcaa ggccgcggcg
     1981 ttaatcttcc agtcgcggac gatctcgcgc gagcgcgccg ggtcgcggta gttgctgctc
     2041 tgcggattgg tcgagttgat ggtcagccac accacgccct tgtcgccgta gcgccgctgc
     2101 agcgactgca tgttgccgct ctcgtaatgc ttctgcacga acgggcactc ggagttgaac
     2161 cattccagca ccacgtactt gccgcggaag gcggaaagct tctgcggctt gccctggatg
     2221 tcggtcaagg tgaagtcggg cgcggcctgg ccgggggcga cgttggccga agcggcgggg
     2281 gcgagcaggg cgaggcccag gctcaggaac atggcggcaa cgggtcgggt catggcgtct
     2341 ccttcgaacg gtgcgacgtg atcatagcgg aagcctgtgc cggccgggat gcggcccgct
     2401 ccagcgcttc gagcaccagc cggcgcgtga gcacctcggg cagcagcacg ggcgggccgc
     2461 ccggcggcga gtacagcgcg tacaccggca cgccgctgcg cttcatgcgc gcgagcgccg
     2521 cggtgatggc gggattgcgg ttggtccagt cggcgcggaa gcgcgctatg ccgagctcca
     2581 cgaagcgccg ctccaccgcc tgcgtgctca acaccaagcg cttgttgacc tgacaggtga
     2641 cgcaccacgc cgccgtgaag tccacgaata cggggcggcc ttgcgccaac gcgctgcgca
     2701 ccgcctcttc cgaccacggt tgccatgcgc tctgtgccgc ctgcacggtc tgctgcaagc
     2761 gccgatcggg ccaggcgacc gcgagcccgg tgaggatcag tacgagcccg atcgctcgcg
     2821 cggcggtgcg tgcattgggc gcggcgtgcc cccagcgccc gaccgcccac agcgcggcgg
     2881 ccaccagcac caagccggcc agcaaccggg caacgccgtc cacgccgacc tgctgtccca
     2941 gcacccatac cagccacacc accgtcgcgt acagcgggaa ggccagcaac tgtcggagcg
     3001 tttccatcca cggcccgggg cgcgggagcc ggcggatcag ttgcggcacg aagcaaagca
     3061 acacgtaggg cgcggccatg cccagcgcca gcgcgctgaa caccagcatc gcgtcgagcg
     3121 ccggctgcac cagagcgaaa cccagcgcgg cgcccataaa cggcgcggtg cagggcgagg
     3181 ccacggcggt ggcgagcatg ccggtcaaca gcgcgtcggc atgggcgccg cgggcgcgca
     3241 tgctgcctgc gagcgactgc accgtgccgc cgaggcggaa cacgccgctc aggttgagcc
     3301 ccagcgcgaa gaacagcagc gccagcgcgg ccacgatcgc cggcgactgc agctgataac
     3361 cccagcccag cgcggcccct tgcgcgcgca acgccagcaa cagcccggcg atcgcccaga
     3421 acgacagcag cacgcccaac gtgaacagca ggccgtggct gcgcaggcgc gcggcccggc
     3481 catgggtctg ctgcaccacg cccagcacct tgatcgccag caccggaaac acgcacggca
     3541 tcaggttgag cagcacgccg cccacgaagg cgagcacgag tgtggtgagc aacgcgacca
     3601 gggtgccgtc gctgcgcgca ggcgcggcga tctcgatggg aacgtcgatc tcgagcgcac
     3661 gcgccgaccc gaagccagat tgcgcaacca gcac
//
LOCUS       DASPGE010000207         6369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_524694, whole genome shotgun sequence.
ACCESSION   DASPGE010000207 DASPGE010000000
VERSION     DASPGE010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524694"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VD791_06645"
     CDS             <1..186
                     /locus_tag="VD791_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HXV07689.1"
                     /translation="FHLPRSTLLMLVAAFAGLDNVLRAYRHAIERRYRFFSYGDAMLI
                     DRNPGLIDRSPGPIDRG"
     gene            195..1298
                     /gene="tgt"
                     /locus_tag="VD791_06650"
     CDS             195..1298
                     /gene="tgt"
                     /locus_tag="VD791_06650"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HXV07690.1"
                     /translation="MQFQLMRSDGSARRGRLTLARGTVETPAFMPIGTYGAVKAVSPA
                     DLRETGAQIILSNTFHLWLRPGLEVIAAHGGLHRFMGWDGPILTDSGGYQVFSLGALR
                     KVAEEGVQFRSPVDGDPCFLSPEEAMRIQRVLDSDVAMVFDECTPYPADESQAAESMR
                     LSLRWAERCARTHAGNPNAVFGIVQGGMYQHLREESVRGLQAIGFDGYAIGGLSVGEP
                     KQEMLRILRHTAPALPAERPRYLMGVGTPEDMVEAVLAGIDLFDCVLPTRNARNGWLF
                     TRAGTIKLRNSRYRTDTRPVDAECGCYTCRGFTRAYLHHLQRVNEMLGARLNTLHNLH
                     YYQELMAGLRAAIERGALQDFVAGFRARLERSA"
     gene            1415..1738
                     /gene="yajC"
                     /locus_tag="VD791_06655"
     CDS             1415..1738
                     /gene="yajC"
                     /locus_tag="VD791_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HXV07691.1"
                     /translation="MISVAYAQAPAPGGGFDLMGMLPLILMFVLLYFLLLRPQMKRAK
                     EHKQMLSSLQKGDEVVTSGGTLGKVTNVGDSYVRVEIAPEIEITVQKPSIQTLLPKGT
                     IKSTT"
     gene            1809..3704
                     /gene="secD"
                     /locus_tag="VD791_06660"
     CDS             1809..3704
                     /gene="secD"
                     /locus_tag="VD791_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HXV07692.1"
                     /translation="MNRFPLWLNLIIAVAVTVGLVYSVPNLFPESPAVQVSASKTSVA
                     LDEALLARVQDALKRAGVPYKDGFVDQTGVKLRFEDTDAQIRAMDVVQAELGGGYTVA
                     LNLLSNAPRWLTAIGAKPMYLGLDLRGGVHFLLQVDMKGAITKALDNSAASIRTTLRG
                     ESEARTSGIVREGNSIALRFREAAARDNAQTIVERNYPDLILRQEERGGEPVLIGTLR
                     PEAEKRILDFAIQQNLTTLRNRVNELGVAEPIVQQAGADRIVVQLPGVQDTARAKDII
                     GRTATLEFRMVEAHMGDARARDFDPAKVQAAVAGNVPFGTELHYSGRGDEREPLLVGK
                     QVIITGDRLSDAAPNFDSQTGQPIVSIRLDGQGARIMTSVTRDAVKRRMAVLLIEKGQ
                     TEVITAPVIQSELGASFQISGSMDTREAQDLALLLRAGALAAPMEIVEERTVGPSLGA
                     ENIERGFRAMWIGFSALSLFIIVYYSVMGVVSVIALAVNLMLLVALLSMLQATLTLPG
                     IAAIALTLGMAIDANVLINERIREELRNGITPQAAIHAGYERAWATILDSNITTLIAG
                     IALFMFGTGPVRGFAVVHCLGILTSMFSAVLVSRAIVNLSFGRKKRLERLPIGNVDWQ
                     KSAPRPA"
     gene            3732..4661
                     /gene="secF"
                     /locus_tag="VD791_06665"
     CDS             3732..4661
                     /gene="secF"
                     /locus_tag="VD791_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HXV07693.1"
                     /translation="MEFFRIRRDIDFMKHAMVFNIISLLVFLLSVFFLITRGLHLGVE
                     FTGGTVMELAYGHSADLDAVRATLEKIELRDATVQSFGSSQNALIRLPARQDLTGAQM
                     SENVMQALRAADPTVELLRVDFVGSQVGKELVENGAIALLLVSLGIVLYLWLRFEWKF
                     GVSAILANLHDVVIILGFFAFFQWEFSVPVLAAVLAVLGYSVNESVIVFDRIRENVRK
                     MRKATLPEVINNAITRTMSRTIITHGSTQMMVTAILIFGGDALFYFALALTIGILFGI
                     YSSVLVASPLLIYLKVSREDLIKPERPKQEAVV"
     gene            4669..5334
                     /locus_tag="VD791_06670"
     CDS             4669..5334
                     /locus_tag="VD791_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HXV07694.1"
                     /translation="MEWLAAFVDLIVHLDEHLLWLAQTYGAWIYAVLFLIVFCETGLV
                     VTPFLPGDSLLFVAGTIAGAGEMNVHLMAGLLVLAAVLGDSLNYAIGQYLGPRVFRYE
                     DSWFFRKAYLERTHAFFERHGGKTIVIARFVPIVRTYAPFVAGIGRMQYGRFLMFNVS
                     GALLWVVLLTYAGYLFGNVPVIKDNLTLVIVVIILLSISPGIIEVLRHRAAARAAQGR
                     RPD"
     gene            complement(5335..6186)
                     /gene="trxA"
                     /locus_tag="VD791_06675"
     CDS             complement(5335..6186)
                     /gene="trxA"
                     /locus_tag="VD791_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HXV07695.1"
                     /translation="MSANALDVNAATFDKEVIDASRTVPVLVDFWAAWCGPCRALKPV
                     LEKLADQYQGKFRLAKVNSDDNPQLSAQYGVRSIPNVKAFVDGELVDEFLGAQPESAV
                     RQFIDGLLPSPGEVLRKRAVALSAAGDAAGALALLEQAAQADGHDDAVYADMVEILLA
                     QGRVDEAKAAAGKLGPLAGDDPRVRALLARLDFTDAGREDAGALRARIEAEPADLEAR
                     LRLAKIDVAGKRYEPALEQLIEIIRRDRKWNGEAGRKTMLAVFDLLGAQHELTGRYRR
                     KLASVLN"
BASE COUNT          979 a         2184 c         2162 g         1044 t
ORIGIN      
        1 ttccatctgc cgcgctccac gctgctgatg ctggtggccg cattcgcggg cctggacaac
       61 gtgctgcgcg cctatcgtca cgcgatcgag cggcgctacc gattcttcag ctacggcgac
      121 gccatgttga ttgatcgaaa ccccgggttg atagatcgaa gccccgggcc aatcgacaga
      181 ggctgacgcc gaatatgcag ttccagctga tgcgcagcga cggcagcgcc cggcgcggcc
      241 ggctcaccct ggcccgcggc acggtcgaga ccccggcctt catgccgatc ggcacctacg
      301 gcgcggtgaa ggcggtcagt cccgccgatc tgcgcgaaac cggcgcgcag atcatcctct
      361 ccaacacctt ccacctgtgg ctgcggcccg ggctggaggt gatcgcggcg cacggcgggc
      421 tgcaccgctt catgggctgg gacgggccga tcctcaccga ttcgggcggc taccaggtgt
      481 tcagcctggg ggcgctgcgc aaggtcgccg aggagggcgt ccagttccgc tcgccggtcg
      541 acggcgaccc gtgcttcctg tcgcccgagg aggcgatgcg catccagcgc gtgctcgact
      601 ccgacgtggc gatggtcttc gacgagtgca cgccctatcc ggcggacgag agccaggccg
      661 ccgaatcgat gcggctcagc ctgcgctggg ccgagcgctg cgcgcgcacg cacgcgggca
      721 atccgaacgc ggtgttcggc atcgtgcaag gcggaatgta ccagcacttg cgcgaggagt
      781 ccgtacgcgg actgcaggcg atcggcttcg acggctacgc catcggcggc ctgtcggtgg
      841 gcgagcccaa gcaggagatg ctgcgcatcc tgcgccacac cgcgccggcc ctgcccgccg
      901 agcggccgcg ctatctcatg ggcgtaggca cgcccgagga catggtggag gcggtgctcg
      961 ccggcatcga cttgttcgac tgcgtgctgc ccacgcgcaa cgcacgcaac ggctggctgt
     1021 tcacccgcgc gggcacgatc aagctgcgca acagccgcta ccgcaccgac acccgcccgg
     1081 tggacgcgga atgcggctgc tacacctgcc gcggtttcac ccgcgcctac ctgcatcacc
     1141 tgcagcgcgt gaacgaaatg ctgggagcgc ggctcaacac tttgcacaat ctgcactact
     1201 accaggagct gatggccggg ctgcgcgcgg cgatcgagcg cggcgcgctg caagatttcg
     1261 tcgccggctt tcgcgcgcgg ctagagcgga gcgcctgagc aaagatgcta gaatccgcgt
     1321 ctttcccggg caggcccggc gatccgcggg cggccagcac cacccgcggg cgggttcctg
     1381 cggcaaggtt gaggtgtcga aggagaccaa ctcgatgatc agcgtagcgt acgctcaggc
     1441 accggcgccc ggaggcgggt tcgacttgat gggaatgctg cctctgatcc tgatgttcgt
     1501 gctgctttac ttcctgctgc tgcgcccgca gatgaagcgc gccaaggagc acaagcagat
     1561 gctctcctcg ctgcagaagg gcgacgaagt ggtgacctcg ggcggcacgc tcggcaaggt
     1621 caccaacgtc ggcgacagct acgtcagggt cgagatcgcg cccgagatcg agatcaccgt
     1681 gcagaagcct tccatccaga cgctgctgcc gaagggcacg atcaagagca ccacatgagc
     1741 cggatcgggc cggggcgccg ggccgcggcc cgcgctccgc gccggacgac tggcgccgca
     1801 ccggacgcat gaaccgcttt cccctctggc tcaacctgat catcgccgtg gcggtgacgg
     1861 tggggctcgt ctactccgtc cccaacctgt ttccggagtc tccggcggtc caggtgtcgg
     1921 cctccaagac cagcgtcgcc ctcgacgagg ccctgctcgc ccgggtgcag gacgcgctga
     1981 agcgcgccgg ggtgccctac aaggacggct tcgtcgacca gaccggcgtg aagctgcgct
     2041 tcgaagacac cgacgcgcag atccgggcga tggacgtggt ccaggcggag ctgggcgggg
     2101 gctacaccgt ggcgctcaac ctgctgtcga acgcgccgcg ctggctcacg gcgatcggcg
     2161 ccaagcccat gtacctgggg ctggacctgc gcggaggcgt gcacttcctg ctgcaggtgg
     2221 acatgaaagg cgccatcacc aaggcgctcg acaactcggc ggcgagcatc cgcaccacgc
     2281 tgcgcggcga gagcgaggcg cgcaccagcg gcatcgtgcg cgagggcaac agcatcgcgc
     2341 tgcgcttccg cgaggccgcc gcccgcgaca acgcccagac catcgtcgag cgcaactacc
     2401 ccgatctgat cctgcgccag gaggagcgcg gcggcgagcc ggttttgatc ggcaccctgc
     2461 gcccggaggc cgagaagcgg atcctcgact tcgccatcca gcagaacctc acgacgctgc
     2521 gcaaccgcgt caacgagctg ggcgtggccg agccgatcgt gcagcaggcc ggcgccgacc
     2581 gcatcgtggt ccagctgccg ggggttcagg acacggcgcg ggcgaaggac atcatcggcc
     2641 gcaccgccac ccttgaattc cgcatggtcg aggcgcacat gggcgacgcg cgcgcccgcg
     2701 acttcgatcc ggccaaggtg caggcggcgg tggccggcaa cgtgccgttc ggcaccgagc
     2761 tgcactactc cggccgcggc gacgagcgcg agccgctgct ggtcggcaag caggtcatca
     2821 tcaccggcga ccgcctgagc gacgcggcgc cgaacttcga ctcccagacc ggccagccga
     2881 tcgtgtcgat ccggctggac gggcagggtg cgcgcatcat gacctcggtc acgcgcgacg
     2941 cggtcaagcg ccgcatggcg gtgctgctga tcgagaaggg gcagaccgag gtgatcaccg
     3001 ccccggtgat ccaatccgag ctgggcgcga gcttccagat ctccggcagc atggacacgc
     3061 gcgaggcgca ggacctggcc ctgctgttgc gcgccggggc gctggccgcg ccgatggaaa
     3121 tcgtcgagga gcgcaccgtc ggccctagcc tgggcgcgga gaacatcgag cgcggtttcc
     3181 gcgccatgtg gatcggcttc tcggcgctgt cgctgttcat catcgtctac tacagcgtga
     3241 tgggcgtggt atcggtgatc gcgctggcgg tgaacctgat gctcctggtg gccctgctgt
     3301 ccatgctgca ggccaccttg accctgcccg gcatcgccgc catcgcgctg accctgggca
     3361 tggccatcga tgcgaacgtg ctgatcaacg agcgcatccg cgaggagctg cgcaacggca
     3421 tcacgccgca ggcggcgatc cacgccggct acgagcgcgc ctgggccacc atcctggact
     3481 cgaacatcac cacgctgatc gccggcatcg cgctgttcat gttcggcacc ggcccggtgc
     3541 gcggcttcgc ggtggtgcac tgcctcggca tcctgacctc gatgttcagc gccgtgctgg
     3601 tgtcgcgcgc catcgtcaac ctcagcttcg ggcgcaagaa gcgcctcgag cgcctgccga
     3661 tcggcaacgt cgactggcag aagagcgcgc cccggcctgc gtagccgccc gatacccgca
     3721 agcagacagc catggaattc ttccggatcc gccgcgacat cgatttcatg aagcacgcga
     3781 tggtgttcaa catcatctcg ctgctggtgt tcctcctgtc ggtgtttttc ctgatcacgc
     3841 gcggcctgca cctgggcgtg gagttcaccg gcggcaccgt gatggagctc gcctacgggc
     3901 attccgccga cctcgatgcg gtgcgcgcca ccctggagaa aatcgagctg cgggacgcga
     3961 ccgtgcagag cttcggctcg tcgcagaacg cgctgatccg cttgcctgcg cgccaggatc
     4021 tgaccggcgc gcagatgagc gagaacgtga tgcaggcgtt gcgcgcggcc gacccgacgg
     4081 tggagctgct gcgcgttgac ttcgtcggct cccaggtcgg caaagagctg gtggagaacg
     4141 gcgccatcgc gctgctgctg gtgtcgctgg gcatcgtgct ctacctgtgg ttgcgcttcg
     4201 agtggaagtt cggcgtgtcc gccatcctcg ccaacctgca cgacgtggtg atcatcctcg
     4261 gcttcttcgc cttcttccag tgggagttct cggtgccggt gctggcggcg gtgctcgccg
     4321 tgctcggcta ttcggtgaac gagtcggtga tcgtgttcga ccgcatccgc gagaacgtgc
     4381 gcaagatgcg caaggccacg ctgccggagg tcatcaacaa cgccatcacg cgcaccatgt
     4441 cgcgcaccat catcacccac ggcagcacgc agatgatggt gaccgcgatc ctgatcttcg
     4501 gcggcgacgc gctgttctac ttcgctctgg cgctcaccat cggcatcctg ttcggcatct
     4561 actcctcggt gctggtggcg agcccgctgc tcatctacct gaaggtctcg cgcgaggacc
     4621 tgatcaagcc cgagcggccc aagcaggaag cggtggtctg actgcgccgt ggagtggctg
     4681 gccgcgttcg tcgacctgat cgtccacctg gacgagcacc tgctgtggct ggcgcaaacc
     4741 tacggcgcct ggatctacgc cgtcctgttc ctgatcgtgt tctgcgaaac cgggctggtg
     4801 gtgacgccgt tcctgcccgg cgattcgctg ctgttcgtgg ccggcaccat cgccggcgcg
     4861 ggcgagatga acgtgcacct gatggcgggg ctgctggtgc tggcggcggt gctgggggat
     4921 tcgctgaact acgccatcgg ccagtacctc ggccccaggg tgttccgcta cgaggactcg
     4981 tggttcttca ggaaggccta cctggagcgc acccacgcct tcttcgagcg ccacggcggc
     5041 aagaccatcg tgatcgcccg cttcgtgccc atcgtgcgca cctatgcgcc gttcgtcgcc
     5101 ggcatcgggc gcatgcagta cggccggttt ctcatgttca acgtgagcgg cgcgctgctg
     5161 tgggtggtgc tgctcaccta tgccggctat ctgttcggca atgtgccggt gatcaaggac
     5221 aacctcacgc tggtcatcgt ggtcatcatc ctgctgtcga tctcgccggg gatcatcgag
     5281 gtgctgcggc accgggccgc cgcgcgcgcg gcgcaaggcc ggcgcccgga ctagtcagtt
     5341 cagcaccgac gcgagcttgc gccgatagcg cccggtgagc tcgtgctgcg cgccgagcag
     5401 atcgaacacg gccagcatgg tcttgcggcc ggcctcgccg ttccatttgc ggtcgcggcg
     5461 gatgatctcg atcagctgct cgagcgcggg ctcgtagcgc ttgcccgcga catcgatctt
     5521 cgccagccgc aggcgcgcct ccaggtcggc gggttccgcc tcgatgcgcg cgcgcaatgc
     5581 gccggcgtcc tcccttccag cgtcggtgaa gtccagccgg gcgagcagtg cgcgcacgcg
     5641 cgggtcgtct cccgcgagcg gaccgagctt gcccgcggcc gccttcgcct cgtccacacg
     5701 tccttgcgcg agcaggatct cgaccatgtc ggcgtacacc gcgtcgtcgt gcccatcggc
     5761 ctgcgcggcc tgctccagca gcgcgagcgc gcccgccgca tcgccggcgg cagacagggc
     5821 gacggcgcgc ttgcgcagga cttcaccggg cgagggcagc aggccgtcga tgaactgccg
     5881 caccgccgac tcgggctgcg cgccgaggaa ctcgtccacc agctcgccgt ccacgaacgc
     5941 cttgacgttc gggatgctgc gcacgccgta ctgcgccgac agctgcggat tgtcgtccga
     6001 gttgaccttg gccaggcgga acttgccctg gtattgatcc gccagcttct cgagcaccgg
     6061 cttcagcgcg cggcaggggc cgcaccacgc tgcccagaaa tccaccagga cggggactgt
     6121 ccgggaggcg tcgatgacct ctttgtcgaa ggtggcggcg ttgacgtcca gggcgttggc
     6181 agacatggat ggctccggcg gatccgatcc cccggcatat cggggcggcg ggcggcgaat
     6241 caagcctgct ctagatgcgc ttggccaggg ccgccgccag cccgaggtag gtgtgcgggg
     6301 tgagcgccag caggcgcgtc ttctcggcct gcggaatggc gaggccgcgg atgaaggcgt
     6361 gcaacgcct
//
LOCUS       DASPGE010000208         6008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1110490, whole genome shotgun sequence.
ACCESSION   DASPGE010000208 DASPGE010000000
VERSION     DASPGE010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110490"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VD791_06680"
     CDS             <1..538
                     /locus_tag="VD791_06680"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HXV07696.1"
                     /translation="APASSLVDVQEGAHIMYVVAGKPDSSVLVASSGGYGFLTKLSDM
                     MSNRRAGREFMSIEEGETPIPPCMVDDAPGNYVAAISEQGRMLLFQVGELKQMAKGRG
                     LVIMGLEQGEKLVQAIVSDRPSLIVSGIARTGKAKDVTIAAERMQHYAGHRARMGRVL
                     PDKLKPSAIQAPPAESDA"
     gene            548..1120
                     /locus_tag="VD791_06685"
     CDS             548..1120
                     /locus_tag="VD791_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07697.1"
                     /translation="MTTKRLHSSPGGEARHGAGASVDRLVRLHPLFVASAVIRGALAL
                     LLVLGAMYVLRLLGTSDLIAWLVLGLTSAGLAVVLLDDALDIEGKVADQAARAAVFEP
                     DGFGVRAAAVREYCDDFVRIRGRIPRARTRIKQPGDNQLFRVYFPSRAADCRRWFATA
                     LLASLHSALRFAAAGLGLVISIQFYFLSGS"
     gene            1244..2992
                     /gene="sulP"
                     /locus_tag="VD791_06690"
     CDS             1244..2992
                     /gene="sulP"
                     /locus_tag="VD791_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HXV07698.1"
                     /translation="MNHPARFALIRHLPILAWGRAYNREDLSADLLAGVITAILLVPQ
                     AMAFGLLAGLPAQAGLYASILPPIVYALFGTSRTLAVGPVSVASIMVAQALAALPAGA
                     DYVSSALLLALLSGVILLVLGLARLGVLANFLSHPVLSGFTSAAAVIIVLNQLPNLIG
                     LPLPGRLPLAELPAAVGAGLARLNLPTLGLGVASVALLLVAGAPLERVLTRAGVSRVR
                     AGVIGKTAPLAVVAVATLLVTGLALDAQHGVAVVGALPGGLPVLALEIPPWLELAQLL
                     PAATLIALVGYVESVSIAKTLANRRRQAIDPNQELLALGAANFAAAVSGGMPVAGGFS
                     RTMVNYHAGARTQAAAIVTAVLVGTVALFFAPLLAHVPKAALAAIIIVAVSRLIDVPS
                     AVAAWRYDRNDGAVLLATAAGVLVLGIEAGLTAGVVLSLLLYIWRASRPHLAVLGRLP
                     GTEHFRNVKRYQELETWPDLLLLRVDEHLSFANSAYLEQVLMEHVARAPKLKHLVLVC
                     SGINGIDFSALEMLLKLISSLKDAHLTVHLAEVKGPVMDKLRATGLPALLEPGRVFLS
                     AHEAVEALAARAVSHG"
     gene            complement(3019..3576)
                     /locus_tag="VD791_06695"
     CDS             complement(3019..3576)
                     /locus_tag="VD791_06695"
                     /inference="COORDINATES: protein motif:HMM:NF042855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6806 family protein"
                     /protein_id="HXV07699.1"
                     /translation="MRTEVHVHGTIPLRSGASLAQIEAALQPWLDYLDVESLDELKSV
                     HRDEPGLVFDPRRRLLEVCWTGDVGRNFRKVIEDALQALCRFTEQATEVQLSYYHEDG
                     RDEIGIVFVGPSPESIHEAQRRRMVEDMSNLLSRHFSQQEVSELVNHVNELFSRNWQQ
                     RGGSAAQIELAAEPVRTSRGRKHLH"
     gene            complement(3807..4187)
                     /locus_tag="VD791_06700"
     CDS             complement(3807..4187)
                     /locus_tag="VD791_06700"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HXV07700.1"
                     /translation="MRHVLSWFTAVALLLAPFAVLSQEEGLKVTEAKLGKGVQDREIT
                     EEASAFALNEKAYLWLRVVGGPSDPIQVTWKTGDHTDTVPLNIGSASWRTWSSKTLWM
                     AGDWTVTVTDAGGQVLQEVNFTVQ"
     gene            complement(4322..>6008)
                     /locus_tag="VD791_06705"
     CDS             complement(4322..>6008)
                     /locus_tag="VD791_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HXV07701.1"
                     /translation="GLRLVAEVDNILHFAELGVVLLLFIIGLELQPSRLWVLRKSVFG
                     LGGAQVLATGLVLAAVGLALDLPWRSALVMGLALAMSSTAFVLQVLAERNQLTTRYGR
                     SAFAILLFQDLSVIPLLALVPLLGTGGAQRGDPWLSAGLALVVIVAVVVGGRLLLRPV
                     LHLIAQSRIQEIFTAAALLVVIGVSLLMTGVGLSMSLGAFLAGVLLADSEYRHELEAN
                     IQPFKGLLLGLFFISVGMSVDLGLIASRPALIVGAVLGLMGLKAALLWALARAAGHGE
                     ESARNLAIALCQGGEFAFVLFNLAAGHAVMERALVDLLVVVVSLSMAATPLALAGNDL
                     LSRRLARARPQRPFDTFEQEAPRVIIAGFGRVGQVVGRILKLKRIAFTALDISPEQVQ
                     TVRRFGNKVHYGDASRLDLLTAAGVGRAKLFVLAIDDVEASIRTARTVRRHFPGLTVY
                     ARARNRFHAHRLMDLGVELIERETFLGSLDLARQVLEAVGTPGWEAQQVVARFQAHDE
                     EALRRQHAVYHDETQLIQSARQAAEELQGLFESDQEDASRVLGTPAFSPSDVR"
BASE COUNT          900 a         2066 c         2110 g          932 t
ORIGIN      
        1 ggcgccggct tcctccctgg tcgatgtgca ggagggcgcg cacatcatgt acgtggtcgc
       61 cggcaagccc gacagcagcg tgctggtggc ctccagcggc ggctacggtt tcctgaccaa
      121 gctctcggac atgatgtcca accgccgcgc tgggcgcgag ttcatgagca tcgaggaagg
      181 cgagacgccg attccgccgt gcatggttga cgatgctccc ggcaactatg tggccgcgat
      241 ctccgaacag ggccgcatgc tgctgttcca ggtcggcgag ctgaagcaga tggccaaggg
      301 ccgcgggttg gtgatcatgg ggctggagca gggcgagaaa ctggtgcagg cgatcgtgtc
      361 cgaccggccg agtctgatcg tgtctggcat cgcgcgcacc ggcaaggcca aggacgtgac
      421 catcgccgcc gagcgcatgc agcactacgc ggggcaccgg gcgcgcatgg gacgggtgct
      481 gccggacaag ctcaagccca gcgcgatcca ggcgccgccg gccgagagcg acgcgtaacg
      541 gcgcgcgatg accaccaagc gcttgcattc ctcccccggc ggtgaagcgc ggcatggcgc
      601 cggcgccagc gtcgaccggc ttgtgcgcct gcatccgctg ttcgtggcga gcgcggtcat
      661 ccgcggggcg ctcgccctgc tgctcgtgct gggagcgatg tacgtgctgc ggctgctcgg
      721 cacctccgat ctgatcgcgt ggctggtgct cggattgacc agcgccgggc tggccgtggt
      781 gctgctcgac gatgcgctcg acatcgaggg caaggtcgcc gaccaggcgg cgcgcgccgc
      841 ggtgttcgag cccgacgggt tcggcgtgcg cgcggcggcg gtgcgtgaat actgcgacga
      901 cttcgtccgg atcagggggc gtatcccgcg cgcgcgcacg cgcatcaagc agccgggcga
      961 caatcagctg ttccgcgtgt actttccctc ccgtgccgcc gattgtcggc gctggttcgc
     1021 cacggcgctg ctggcctcgc tgcacagcgc gctgcgtttc gctgccgccg gcctcgggct
     1081 ggtgatctcg atccagttct acttcctgtc cgggagctag cgctccggcg ggcgtcccgc
     1141 tgcggcggtc gagcccgatc tgacgcgtcc agaacaggcg ataattccgc gtcctgatga
     1201 aatatccggg ctagaatcgg ctccgcgctt ttgcgctgcg accgtgaacc acccagcccg
     1261 cttcgcactg atccgccacc tgccgatcct ggcctggggc cgtgcctaca accgcgagga
     1321 tctgagcgct gatctgctcg ccggcgtgat caccgccatc ctgctggtgc cgcaggcgat
     1381 ggccttcggg ctgctcgccg ggctgccggc gcaggccggg ctgtatgcga gcatcctgcc
     1441 gccgatcgtg tacgccctgt tcggcaccag ccgcactctg gcggtgggcc cggtgtcggt
     1501 ggcctcgatc atggtggcgc aggcgctggc cgcgttgccg gcgggagcgg attacgtgtc
     1561 cagcgcgctg ctgctggcgc tgctgtctgg ggtcatcctg ctggtgctcg gcctggcgcg
     1621 gctgggggtg ctggcgaact tcctcagcca cccggtgctg tcggggttca ccagcgcggc
     1681 ggccgtgatc atcgtgctca accagctgcc gaacctgatc ggattgccct tgcccggccg
     1741 attgcctctc gccgaattgc ccgcggcggt cggcgcgggc ctggcacggt tgaatctgcc
     1801 cacgctcggg ctgggcgtgg cgagcgtcgc gctgctgctg gtcgcgggtg cgccgctgga
     1861 gcgcgtgctg acccgcgccg gcgtctctcg cgtgcgcgcg ggtgtgatcg gcaagaccgc
     1921 tcccctcgcg gtggtggcag tcgcgacatt gctggttacc ggtttggctc tcgatgcgca
     1981 gcacggcgtc gcggtcgtgg gggcattgcc cgggggcctg cccgtactgg cgctcgagat
     2041 tccgccatgg ttggaactgg cgcagctgct gccggccgcg acgcttatcg cgctggtggg
     2101 ctacgtcgag agcgtgtcga tcgccaagac gctggccaat cgtcggcgcc aggcgatcga
     2161 cccgaatcag gagctgctgg cgctgggggc ggccaacttc gccgccgccg tgtcgggcgg
     2221 catgccggtg gcgggcggct tcagccgcac catggtcaac taccacgccg gcgcgcgcac
     2281 gcaggccgcg gccatcgtca ccgcggtgct ggtcggcacg gtcgcgctgt tcttcgcacc
     2341 gctgctcgcg cacgtgccga aggcggcgct ggcggccatc atcatcgtcg cggtgtctcg
     2401 gctgatcgac gtgcccagcg cggtggccgc gtggcgctac gaccgcaacg acggtgcggt
     2461 gctgctggcc accgccgccg gcgtgctggt gctcgggatc gaggccgggc tcaccgccgg
     2521 ggtggtgctg tcgctgctgc tgtacatctg gcgcgcttca cggccgcatc tggccgtgct
     2581 cgggcgcctg cccggcaccg agcacttccg caacgtcaag cgctaccagg agctggagac
     2641 ctggcccgac ctgctgctgc tgcgcgtgga cgagcacctc tctttcgcca acagcgccta
     2701 cctcgagcag gtgctgatgg agcacgtggc gcgcgcgcca aagctcaagc atctggtgct
     2761 ggtgtgcagc ggcatcaacg gcatcgactt cagcgcgctg gagatgctgc tgaagctgat
     2821 ctcctcgctg aaggacgcgc acctgaccgt gcacctggcc gaggtcaagg gcccggtgat
     2881 ggacaagctg cgcgccaccg gcctgccggc gctgctcgaa cccgggcgcg tcttcctcag
     2941 cgcgcacgaa gcggtggaag cgctggccgc tcgcgccgtg tcgcacggct gaccttgggg
     3001 ttctcagtgc aggggggctc agtgcaggtg cttgcggccg cgcgaggtgc gcaccggctc
     3061 ggcggccagc tcgatctgcg cggcgctgcc gccgcgctgc tgccagttgc gcgagaacag
     3121 ctcgttgacg tgattcacca gttcggagac ttcctgctgg ctgaagtggc gcgacagcag
     3181 gttggacatg tcctccacca tgcgccggcg ctgcgcctcg tggatgctct ccgggctggg
     3241 gccgacgaac acgatgccga tctcgtcgcg cccgtcctcg tggtagtagc tcaactgcac
     3301 ctcggtggcc tgctcggtga agcggcacag cgcctgcagc gcgtcctcga tcaccttgcg
     3361 gaagttgcgc cccacatcgc cggtccagca cacctcgagc agccggcggc gcggatcgaa
     3421 caccagtccg ggctcgtccc ggtgcacgct cttcagctcg tcgaggctct ccacgtcgag
     3481 gtagtcgagc caaggctgca gcgcggcttc gatctgcgcc agcgaggcgc cggagcgtag
     3541 cggaatggtg ccgtggacgt ggacttcggt gcgcataggg cggggtcggg gagttggatt
     3601 ttctggaaac cggattcgcg ggcggccgct cggcgggcag cgcccagtat acaggtccgc
     3661 ggcggccccg gcggcagccg tgtgaccaag tagcggcgcg ccccgggtca cccgggcggc
     3721 gcgcttcggt ggggagaagg gaaggggacg gggaaactta cccgggcccc gtcagacccg
     3781 gattgctgac cggaccattg ccccggtcac tgcacggtga agttgacttc ctgcagcact
     3841 tggccgccgg cgtcggtgac ggtgacggtc cagtcgcccg ccatccacag cgtcttgctg
     3901 gaccaggtgc gccagctggc gctgccgata ttgagcggaa cggtgtcggt gtggtcgccc
     3961 gtcttccagg tgacctggat cgggtcggac gggccgccga caacgcgcag ccacaggtag
     4021 gccttctcgt tcaacgcgaa ggcgctcgct tcctcggtga tctcgcgatc ctgcacgccc
     4081 ttgccgagct tggcctcggt gaccttcagg ccctcttcct gggacagcac cgcgaacggc
     4141 gccagcagca gggcaacggc ggtgaaccat gacagaacgt gtcgcatgca gacctccttg
     4201 aatgacgggc ccggacggcg ggccggcgcc gatgggacag gcgcggagga agcaagttcc
     4261 atgcgcctgg catgacgccc gcgggcgaca cgcgacgtcc acgagagctt gcatcgacgt
     4321 atcagcgcac gtcggaagga ctgaatgccg gcgtgccgag cacgcgcgag gcgtcctcct
     4381 gatcggactc gaacagcccc tgcagctcct cggccgcctg ccgcgcgctt tgaatcagct
     4441 gcgtctcgtc gtgatagacg gcatgctggc gccgcaacgc ctcctcgtcg tgcgcctgga
     4501 agcgtgccac gacctgctgc gcttcccagc ccggcgttcc gaccgcttcc agcacctgcc
     4561 tggcgagatc gagtgagccg aggaaggtct cgcgctcgat cagttccacg cccaggtcca
     4621 tcaggcgatg agcgtggaag cggttgcgcg cacgcgcgta caccgtgagc ccgggaaagt
     4681 gccggcgcac ggtccgcgcg gtgcggatcg acgcctcgac gtcgtcgatg gcgagcacga
     4741 acaacttggc gcggccgacc ccggccgcag tgagcagatc caggcgcgaa gcgtcgccgt
     4801 agtggacctt gttgccgaag cgacgcaccg tctgcacctg ctcagggctg atgtcgagcg
     4861 cggtgaaggc gatgcgcttg agcttcaaaa tgcgacccac gacctggccc acgcggccga
     4921 agccggcgat gatgacgcgc ggcgcctctt gctcgaacgt gtcgaacggc cgctgcggcc
     4981 ttgctcgcgc gagccggcgt gagagcaggt cgttgcccgc cagcgccagc ggcgtggccg
     5041 ccatcgacag ggacacgacc acgaccagca agtcgaccag cgcgcgctcc atgaccgcgt
     5101 ggccggcggc caggttgaac aacacgaagg cgaactcgcc gccctggcac aacgcgatcg
     5161 cgaggttgcg cgccgactcc tcgccgtgtc cggcggcacg cgccagcgcc cacagcagcg
     5221 ccgccttcaa ccccatcagg cccagcacgg cgccgacgat gagcgcaggc ctggatgcga
     5281 tcaagcccaa gtccaccgac atgccgaccg agatgaagaa caagcccagc aacagaccct
     5341 tgaacggctg gatgttcgcc tccagctcgt ggcggtactc cgagtcggcc agcagcacgc
     5401 cggccaggaa ggcgccgagc gacatcgaca gccccacgcc ggtcatcagc agagacacgc
     5461 cgatcaccac cagcaacgcc gcggcggtga agatctcctg gatcctggac tgggcgatca
     5521 ggtgcagcac gggccgcagc agcaggcgtc ccccgacgac cacggcgacg atgaccacga
     5581 gcgcgagacc cgcggaaagc cagggatcgc cgcgctgcgc gccgccggta ccgagcaggg
     5641 gcaccagcgc cagcagcggg atgaccgaca ggtcctggaa cagcaggatc gcgaacgccg
     5701 agcgtccata gcgcgtggtg agctgattgc gttcggccag cacctgcagc acgaaggcgg
     5761 tggaggacat ggcgagcgcg agccccatca ccagcgcgct gcgccacggc aggtccagcg
     5821 ccaggcccac cgccgccagc accaagccag tggccaggac ctgcgcaccg ccgagcccga
     5881 acaccgactt gcgcagcacc cacaggcgcg agggctgcag ctcgaggccg atgatgaaca
     5941 gcagcagcac cacgcccagc tcggcgaagt gcagaatgtt gtcgacctcg gcgaccagcc
     6001 gcagcccc
//
LOCUS       DASPGE010000209         3407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_759040, whole genome shotgun sequence.
ACCESSION   DASPGE010000209 DASPGE010000000
VERSION     DASPGE010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759040"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VD791_06710"
     CDS             complement(<1..758)
                     /locus_tag="VD791_06710"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HXV07702.1"
                     /translation="MHLIRPFAGLRPAPAYAEAVAAPPYDVLSAAEARRRAAGKPWSF
                     LHISRPEIDLPEEIDPHSPAVYAKAADTLRRMLREGVLVRDPAPCYYVYRLIQGDHVQ
                     TGLVTAASVRAYDANRIRRHELTRPDKEDDRVRQIETLGAQTGPVLLAYPAAPAVDTL
                     LVQASSAAPATDIVADDGVRHTIWLVGDASIIERLTAAFDAMPALYIADGHHRSAAAS
                     RVAAARRAADSRSDAERGDDGFLAAAFPHHQMRIL"
     gene            1026..2258
                     /locus_tag="VD791_06715"
     CDS             1026..2258
                     /locus_tag="VD791_06715"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXV07703.1"
                     /translation="MPQSFLRGLSGFALPLLLLLVAHFSVPYAADLPYSLVGLRTWGP
                     LLMLALAGTLALAFNRGRAVFAVVSLIAAFVAYRLHLHEGLAPFSARTLFAAVCLFVP
                     LNLATLSVLLERGVFTFYGARRLGSIALQAAVTAWVIEAQRTEITEWIYRPWIEAPLL
                     AGALIPQLALLVLLLGVIVTVSGGVVHRSAIDAGFAGALVAFALACEGIDTPHYFAVY
                     LTASGAILAAAVLQDTFRLAFRDELTGLPSRRALNERMMALGPNYTVAMLDVDHFKRF
                     NDMHGHELGDQVLRMVATKLERIGGGGRAYRYGGEEFIVLFPGKRLRDAWPHLEGLRK
                     AIARHQVVVRGADRPEQVPETPLPAGTRHPYATVSVAVSIGVAERNAGLGTPSEVLRA
                     ADEALYRAKEKGRNRLSR"
     gene            2345..>3407
                     /locus_tag="VD791_06720"
     CDS             2345..>3407
                     /locus_tag="VD791_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07704.1"
                     /translation="MTTAFGALRRLVPGALRRLVPGALRWLVPGWLAFAPFLLCLAPH
                     AAAEELRMRFKAWSVTCAGERYCSASTRLRSDDPQLRYAYLLRVSRYESGEREIAFLP
                     AQERPAADAEIAVRVDGNAAVRLAPESGWRAVGGGNTVLVAGADQVASLLDTMRTGKR
                     VELRYRSTRDKSVEVSFPLAGFAPALAVVDPRPVKRAQPKPDTQSAAAPGDLSAAMSD
                     SVAAASDTLTAASDPAAAVVAARAEPEPSVEVVPEVAPVRADPPANPALAQAKPKPAS
                     KPASASGPPPPGRKRVKAVRQFTCRGDEPAWNLMIDNNRGSFELLLDEEPAIVALTGR
                     LGVTGEGRTPDVDWRGKSAD"
BASE COUNT          484 a         1198 c         1221 g          504 t
ORIGIN      
        1 aggatgcgca tctggtggtg tggaaaggcg gccgccagga aaccgtcgtc gccgcgctcg
       61 gcgtccgacc gggaatccgc ggcgcggcgt gcagccgcga cgcgcgaggc ggcggccgag
      121 cggtgatgcc cgtccgcgat gtacaaggcc ggcatggcgt cgaacgccgc ggtcaaccgc
      181 tcgatgatcg aggcgtcgcc gaccagccag atggtgtggc gcacgccgtc gtcggcgacg
      241 atgtcggtcg ccggcgctgc gctggaggcc tgcaccagca gcgtgtcgac cgcaggcgcc
      301 gccggatagg ccagcagcac cggcccggtc tgcgccccga gcgtctcgat ctggcgcacg
      361 cggtcgtcct ctttgtccgg gcgcgtgagc tcgtggcggc ggatgcggtt ggcgtcgtac
      421 gcgcgcaccg acgcggccgt caccagcccg gtctggacat gatccccctg gatcaagcgg
      481 tacacgtagt agcagggcgc gggatcgcgc accagcacgc cttcgcgcag catgcgccgc
      541 agcgtgtcgg cggccttcgc gtacaccgcc ggcgaatgcg ggtcgatctc ctccggcaaa
      601 tcgatctccg gcctggagat gtgcaggaag ctccacggtt tgccggccgc gcgccggcgc
      661 gcctccgccg cggacagcac gtcgtagggc ggggcggcca ccgcctcggc ataagccggc
      721 gccggccgca agccggcgaa ggggcggatc aggtgcaaaa gtttcagacc cttgcaacga
      781 aaaggcgcga ttctaccccg gacgcggccc ggcaacgtgc gcttcttgaa cggccccctc
      841 ccgaacaacc aggcaagtca agtgtcggag cgatgcgcgc agttcaggca aagaccttgc
      901 acggaatccc gcgtcgacgg cgcatccccc gcaaacaaac ccgttttcgt tgcccgggga
      961 cgacaagggt gccctctttc gtccacttca caccaccggg gtacggtttc tccgggagcg
     1021 ccgaattgcc gcaatcgttc ctgcgtggct tgtcggggtt tgcgctgccg ctgctgctgt
     1081 tgctggtggc gcacttcagc gtgccctacg ccgccgacct gccgtactcc ctggtcgggc
     1141 tgagaacctg gggcccgctg ctgatgctgg cgctcgccgg cacgctggcg ctcgccttca
     1201 accgcgggcg cgcggtgttc gcagtggtgt cgctcatcgc cgcctttgtc gcctaccggc
     1261 tgcatctgca cgaggggctg gcgccgttct ccgcgcgcac cctgttcgcg gccgtctgcc
     1321 tgttcgtgcc gctgaacctg gccaccctga gcgtgctgct cgagcgcgga gttttcacgt
     1381 tctacggcgc acgccggctg ggcagcatcg ccctgcaggc ggccgtgacg gcctgggtca
     1441 tcgaggcgca gcgcacggag atcaccgagt ggatctaccg cccctggatc gaagcccctc
     1501 tgctcgccgg ggcgctgatc ccgcagctcg cgttgctggt gctgctgctc ggcgtgatcg
     1561 tcaccgtgag cggcggcgtc gtgcaccgca gcgccatcga cgccggcttt gccggagcgc
     1621 tggtggcctt cgcgctggct tgcgagggca tcgacacgcc gcattacttc gccgtgtatc
     1681 tcaccgccag cggcgcgatc ctggccgccg cggtgctgca ggacaccttt cgtctggcct
     1741 tccgcgacga gctcaccggg ttgcccagcc ggcgggcgct caacgagcgc atgatggcgc
     1801 tcgggcccaa ctacaccgtc gccatgctcg acgtggacca cttcaagcgc ttcaacgaca
     1861 tgcacggaca cgagctgggc gaccaggtgc tgcgcatggt ggcgacgaag ctcgagcgca
     1921 tcggcggcgg cggccgcgcc tatcgctacg gcggcgagga gttcatcgtg ctgttcccgg
     1981 gcaagcggct gcgcgacgca tggccgcatc tggaagggct gcgcaaggcg atcgcccgtc
     2041 atcaggtggt ggtgcgcggc gcggaccggc ccgagcaagt acccgagacc ccgctgcccg
     2101 ccggcacccg acacccgtat gcgaccgtgt cggtcgcggt cagcatcggc gtggccgagc
     2161 gcaacgccgg cctcggcacg ccctcggagg tgctgcgcgc ggccgacgag gcgctgtatc
     2221 gcgccaagga gaagggccgc aaccgactga gcaggtagcg agtggcagcg actgagcaga
     2281 tggcgcatgg agacggcgcc gttgccctta caatagggcg cgcatccact gccccgactc
     2341 cgtcatgacc accgccttcg gcgcgctgcg ccggctcgtc ccaggcgcgc tgcgccggct
     2401 cgtcccaggc gcgctgcgct ggctcgtccc aggctggctc gcctttgcgc cgttcctgct
     2461 gtgcctggcg ccgcacgccg cggccgaaga gttgcgcatg cgcttcaagg cctggtcggt
     2521 cacctgcgcc ggcgagcgct actgcagcgc cagcacgcgg ctgcgctcgg acgatccaca
     2581 attgcgctac gcatacctgc tgcgggtatc gcgctacgaa tcgggcgaac gcgagatcgc
     2641 cttcctgccg gcgcaggagc ggccggccgc cgatgcggag atcgccgtgc gcgtggacgg
     2701 caacgcggcc gtgaggctcg ccccggaaag cggttggcgc gccgtgggcg ggggcaacac
     2761 cgtcctggtc gccggcgccg atcaagtggc gagcctgctc gatacgatgc gcaccggcaa
     2821 gcgcgtggag ctgcgctacc gctcgacgcg cgacaagtcg gtggaagtga gctttccgct
     2881 ggcgggcttt gcgccggcgc tcgcggtggt cgatccgcgg ccggtgaagc gcgcgcagcc
     2941 gaaaccggac acgcagtccg ccgcggcgcc gggtgatctg tctgcagcga tgagcgattc
     3001 ggtcgcagcg gcgagcgata cgctgacagc ggcgagcgat ccggccgcag cggtggtggc
     3061 ggcgcgcgcc gagccggagc cgtcggtgga agtggtgccg gaggtcgcgc cggtgcgggc
     3121 cgacccgccg gcgaacccgg cgctcgcaca ggcgaagccc aagccggcga gcaagccggc
     3181 cagcgccagc ggcccgccgc cgccggggcg caagcgcgtc aaggcggtgc ggcagttcac
     3241 gtgccgcggc gacgagcccg cctggaatct catgatcgac aacaaccggg ggagtttcga
     3301 actgctgctc gacgaagaac cggcgatcgt cgcgctcacc ggcaggctcg gcgtcaccgg
     3361 agagggccgc actcccgacg tggattggcg cggcaagtcc gcggacg
//
LOCUS       DASPGE010000210         6501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_408098, whole genome shotgun sequence.
ACCESSION   DASPGE010000210 DASPGE010000000
VERSION     DASPGE010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408098"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="VD791_06725"
     CDS             <1..1058
                     /locus_tag="VD791_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXV07705.1"
                     /translation="PEASVRMTHTINIAGDPQPVPAEDGETVLAALLRNGVGFAYSCE
                     AGNCGTCKCEYVSGDILELEYSEHALSAAERARNIVLACRSQVWSDVEIRRLTAEDFV
                     MHPSRVMQCRVSASEMLTHDVLMLRFTIESGGPYTFSAGQFAKLQFGFAPDAPRDYSM
                     ASTPADAGLEFHIRVLASGVSGQIRDRVRIGDLVRISGPFGTSYLREQHNGPIVAIAG
                     GTGLAPVRSIVRTALDKGLPGPVHLYFGVRAERDVYGEAELRTWEAHHPNLKVHVVLS
                     EAAQLTDQHRAGMVTDAVRADFDSLAGFHAYLAGPPAMVDAATELLREKGVGARDMHA
                     DAFYSAPSAPANKRATA"
     gene            1055..1981
                     /locus_tag="VD791_06730"
     CDS             1055..1981
                     /locus_tag="VD791_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV07706.1"
                     /translation="MKSEFSLEGRVALVTGGARGIGRAIAGELARQGAKLVIADPGVN
                     IGGGERDPALVDNAAREVGGVALAQDIGAPGAAQAAVALAVKHFGAIDIVVNNAAILR
                     DAFIFKAQREDWERVIQTDLTGAFATLAAATPWMREAVKQGRAPGRIVNMVSTAGLYG
                     NFAQCAYAAAKAGLVGLTRSAAMDLARVQIHCNAVAPFAATRVTESIQPANEAQAAYK
                     ARALTVPAGYVARLVAWLCAPACAVTGQIFGVRGREVFLFSQPRPVARVVTPPLDELT
                     SEALDNLVSLEMRERFTDLTTDLEAFNTEPLL"
     gene            1994..2695
                     /locus_tag="VD791_06735"
     CDS             1994..2695
                     /locus_tag="VD791_06735"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HXV07707.1"
                     /translation="MSKTATLDVKTVAELKQQPQEYQTAVEKIVISHAVNELYGAQVF
                     DEPAIQLAPTPYAKWLTCRVAMEEYHHHVRFKALADEIGITPERMDPSRKKPLTIFQF
                     ALKTWPEFCVIKAIADYAEILQVEDLLHCSFHPLRNLGRITMPEEKFHAKFGKDSCLE
                     LIQAGRGAEVQDAIDRYFPITPAFFGASQSKNNEMFRKWALKQRTNDEMRADFLARVT
                     ELVEKDFALRLPALN"
     gene            2723..3769
                     /gene="gtdA"
                     /locus_tag="VD791_06740"
     CDS             2723..3769
                     /gene="gtdA"
                     /locus_tag="VD791_06740"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006215999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HXV07708.1"
                     /translation="MAKPASDPRAARAAYYRRIAEQHLTPLWEVLAALVPEQPQSPCV
                     PAIWRYEALRPWLMESGRLISAQEAVRRVLILENPGMPGSSAITQSLYAGLQLILPGE
                     VAPSHRHTQSALRLIVEGSGAYTAVEGERALMHPGDFIITPSWTWHDHGNEASGPVVW
                     LDGLDIPLVRFLDCGFSENYPQASQPVARPEGDALARYGNNLLPVDYEPAAQSSPVFA
                     YPYARTRESLALLQRNDAPHPAHGHKMQFINPATGGYAMPTMAAFVQLLPAGFSGVGY
                     RSTDAAVFHVIEGSGSALIGDTRIDFAVRDTFVVPAWRAYRLRAQAECVLFSFSDRPV
                     QRAAGLWREQTLAA"
     gene            3766..4311
                     /locus_tag="VD791_06745"
     CDS             3766..4311
                     /locus_tag="VD791_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07709.1"
                     /translation="MIAVLPHQADAAWFACLGLPLAAGDLRDAAAYLAALGYPPSTPI
                     RGVPDWPAALALARDPAWDRRWWDEEQAERARLMRVAEQRLGRDALLERLSAGTELAG
                     QAIHGAAAIAAAIANNGRDAVADAALVRAASGAASMALNAATLARLAGQGPEHLFMRK
                     YALFESGRWPLGVVGSAFHLF"
     gene            4528..5223
                     /locus_tag="VD791_06750"
     CDS             4528..5223
                     /locus_tag="VD791_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXV07710.1"
                     /translation="MAELYVIDLPAVPSIPVTGQKARFPVHRIYCVGRNYAAHAREMG
                     KDPEKEPPFFFMKPADAVLPEGGEMHYPPGTANLHHEIELVVALHRGGFKIPAARALD
                     HVYGYAVGLDMTRRDLQTQARDMGRPWDFGKAFDESAPISAIYPVASHGHHSAGAIAL
                     KVNGELRQSGDLQDMIWNVPDTIAFLSEYYRLQPGDLIFTGTPAGVGAVKQGDVLVGA
                     VEGLTELRVTIVP"
     gene            5363..6259
                     /locus_tag="VD791_06755"
     CDS             5363..6259
                     /locus_tag="VD791_06755"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HXV07711.1"
                     /translation="MPTPKTWLEVSLNGPWGRGRQPRIPVSVDEIVREGVACAEAGAA
                     IVHVHAYDEASGKPQENAETYARIIGGIRAACDAIVYPTIPVLGLSPFPKEATAAQRF
                     ATVEALAGRGLLEWAAVDPGSVNLAYYDDLREDREGFVYLNPEDHIRHALGLARTHRF
                     HPAYAIYEPGFLRLGATLHWRCSSPAPLYRFMFSSGYTFGFPPDDYGLTAYLTLLDQV
                     APGAQWMVGGLSVDVLPLIPRIVAEGGHVRVGLEDAPWGSQRSNLDWVREAARRIDNA
                     GGELATPADVRAVMRAVEMGDA"
     gene            6300..>6501
                     /locus_tag="VD791_06760"
     CDS             6300..>6501
                     /locus_tag="VD791_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HXV07712.1"
                     /translation="MAASAKIGITMGDPSGIGPEIIVKALKEMAPEQRSATAVIGNLD
                     ILKRADRLVGAGLSYVEGVDAAR"
BASE COUNT         1009 a         2235 c         2285 g          972 t
ORIGIN      
        1 gcccggaagc aagcgtgcgc atgacgcata ccatcaacat cgcgggcgat ccccagccgg
       61 tgccggcgga ggacggcgag accgtccttg ccgcactgct gcgcaacggc gtgggcttcg
      121 cctacagctg cgaggccggc aactgcggca cctgcaagtg cgagtacgtc tccggcgaca
      181 tcctcgagct ggagtattcc gagcacgccc tgtcggccgc ggagcgcgcc cgcaacatcg
      241 tgctcgcgtg ccgctcgcag gtgtggagcg acgtcgagat ccggcggctg accgccgagg
      301 acttcgtgat gcacccctcg cgcgtgatgc agtgccgggt cagcgccagc gagatgctca
      361 cgcacgacgt gctgatgctg cgcttcacca tcgaatcggg cggtccctat acgttctccg
      421 ccggccagtt cgccaagctg cagttcggct tcgcgcccga cgcgccgcgc gattactcca
      481 tggcgagcac gccggccgac gccgggctcg agttccacat ccgcgtgctg gccagcggcg
      541 taagcggcca gatccgcgat cgcgtgcgga tcggtgacct ggtgcgcatc agcggcccgt
      601 tcggcacttc gtatctgcgc gagcagcaca acggtccgat cgtggccatc gccggcggca
      661 ccgggctggc accggtgcgc tccatcgtgc gcaccgcgct ggacaagggg ctgcccgggc
      721 cggtccacct gtatttcggg gtgcgcgcgg agcgcgacgt gtacggcgaa gccgagctgc
      781 gcacctggga agcgcaccat cccaacctga aagtacacgt ggtgctgtcg gaggcggcgc
      841 agctcaccga tcagcaccgc gccggcatgg tcaccgacgc ggtgcgcgcc gatttcgaca
      901 gcctcgccgg cttccacgcc tatctcgccg ggcctccggc gatggtggac gcggccaccg
      961 agctgctgcg cgagaaggga gtcggcgcgc gcgacatgca cgccgacgcg ttctacagcg
     1021 cgccgtccgc gccggccaac aagagggcga ctgcatgaag agcgagttca gcctggaggg
     1081 acgggttgcg ctggtgacgg gcggggcgcg cggcatcggg cgcgccatcg ccggggagct
     1141 ggcgcgccag ggcgccaagc tggtgatcgc cgaccccggc gtcaacatcg gcggcggaga
     1201 gcgcgatccg gcgctggtgg acaacgccgc gcgcgaggtg gggggcgtcg cgctcgccca
     1261 ggacatcggc gctccgggcg cggcgcaggc ggcggtggcg ctggcggtga agcatttcgg
     1321 cgccatcgac atcgtggtca acaacgccgc catcctgcgc gacgccttca tcttcaaggc
     1381 gcagcgcgag gactgggagc gcgtgatcca gaccgacctc accggcgcct tcgccacgtt
     1441 ggcggccgcc acgccctgga tgcgcgaggc cgtcaagcag ggacgcgcgc ctgggcgcat
     1501 cgtcaacatg gtgtccaccg ccgggctgta cggcaacttc gcccagtgcg cctacgccgc
     1561 ggccaaggcc gggctggtgg ggctgacgcg cagcgccgcg atggacctgg cgcgcgtgca
     1621 gatccactgc aacgcggtgg cgccgttcgc ggccacgcgc gtcaccgagt cgatccagcc
     1681 cgccaacgag gcgcaggccg cctacaaggc gcgcgcgctc acggttcccg ccggctacgt
     1741 ggcgcgcctg gtcgcctggc tgtgcgcgcc cgcctgtgcg gtgaccggcc agatcttcgg
     1801 ggtgcgcggg cgcgaggtat tcctgttctc gcaaccacgg ccggtggcgc gcgtggtcac
     1861 gccgccgctc gacgagctca cgtccgaggc gctggacaac ctggtgagcc tggagatgcg
     1921 cgagcggttc accgacctga ccaccgatct ggaagccttc aacaccgaac cgttgcttta
     1981 gaggaacaac gcgatgtcca agaccgccac gctcgacgtc aagaccgtcg ccgagctgaa
     2041 gcagcagccg caggagtacc agaccgcggt ggaaaagatc gtgatcagcc acgccgtgaa
     2101 cgagctctac ggcgcgcagg tgttcgacga gccggcgatc cagctcgcgc ccacgccgta
     2161 cgcgaagtgg ctcacctgcc gcgtggcgat ggaggagtac catcaccacg tgcgcttcaa
     2221 ggcgctggcc gacgagatcg gcatcacgcc cgagcgcatg gatcccagcc gcaagaagcc
     2281 gctcaccatc ttccagttcg cgctcaagac ctggccggag ttctgcgtga tcaaggccat
     2341 cgccgactac gccgagatcc tgcaggtgga agacctcctg cactgcagct tccacccgct
     2401 gcgcaacctc gggcgcatca ccatgccgga ggagaagttc cacgccaagt tcggcaagga
     2461 ctcctgcctg gagctgatcc aggccggccg cggcgccgag gtgcaggacg cgatcgaccg
     2521 ttatttcccg atcacgccgg cgttcttcgg cgcgagccag tcgaagaaca acgagatgtt
     2581 ccgcaagtgg gcgctcaagc agcgcaccaa cgacgagatg cgcgccgact tcctggcgcg
     2641 cgtgaccgag ctggtggaaa aggacttcgc attgcgtctg cccgcgctga actgaaccgc
     2701 gggcaagatc tgggggtaga gcatggccaa accggcaagc gacccgcgcg cggcgcgcgc
     2761 agcctactac cgccgcatcg ccgagcagca tctgacgccg ctgtgggaag tgctcgcggc
     2821 gctggtgccg gagcagccgc agtcgccctg cgtgccggcg atctggcgct acgaggcgct
     2881 gcggccctgg ctgatggagt cggggcggct gatcagcgcg caggaggcgg tgcgccgcgt
     2941 gctgatcctg gagaaccccg gcatgcccgg ctcgtcggcg atcacccagt cgctgtacgc
     3001 cgggctgcag ttgatcctgc ccggcgaggt cgctcccagc caccgccaca cgcagtcggc
     3061 gctgcgcctg atcgtcgagg gcagcggcgc gtacaccgcg gtggagggcg agcgcgcgct
     3121 gatgcacccg ggcgatttca tcatcacgcc ttcctggacc tggcacgacc acggcaacga
     3181 ggcgagcggc ccggtggtgt ggctggacgg gctggacatt ccgctggtgc gcttcctcga
     3241 ctgcggcttc tccgagaact atccgcaggc ctcgcagccg gtggcgcgcc ccgaggggga
     3301 tgcgctcgcg cgctacggca acaacctgct gccggtggat tacgagccgg ctgcgcagtc
     3361 ctcgccggtg ttcgcctatc cgtatgcgcg cacgcgcgag tcgctcgcgc tgctccagcg
     3421 caacgacgcg ccgcatccgg cgcacggcca caagatgcag ttcatcaacc cggccaccgg
     3481 cggctacgcc atgccgacca tggccgcgtt cgtgcagctg ctgcccgccg ggttctccgg
     3541 ggtcggttac cgcagcaccg acgccgccgt gttccacgtc atcgagggca gcggcagcgc
     3601 cctgatcggc gacacgcgca tcgacttcgc cgtacgcgac acgttcgtgg tgccggcctg
     3661 gcgcgcctat cgcctgcggg cgcaggccga gtgcgtgctg ttcagcttct ccgaccggcc
     3721 ggtgcagcgc gccgcgggtc tgtggcgcga acagacgctc gcggcgtgat cgccgtattg
     3781 ccgcaccagg ccgacgccgc ctggttcgcg tgcctgggcc tgccgctcgc ggcaggcgat
     3841 ctgcgcgacg cggccgcgta tctggcggcg ctcggctatc cgccttcgac gccgatccgt
     3901 ggtgttccgg actggccggc cgcgctggcg ctggcgcgcg acccggcctg ggaccggcgc
     3961 tggtgggacg aggagcaggc cgagcgagcg cgcctgatgc gcgtcgccga gcagcggctg
     4021 gggcgcgacg cgctgctcga gcggctcagc gccggcaccg agctcgccgg acaggcgatc
     4081 cacggcgccg ccgccatcgc tgccgccatc gccaacaacg ggcgcgacgc cgtcgccgac
     4141 gccgcgctgg tgcgcgcggc ctccggcgcc gccagcatgg cgctgaacgc cgccaccctg
     4201 gcgcggctcg cggggcaggg gccggagcat ctcttcatgc gaaaatacgc gctgttcgag
     4261 tccgggcgct ggccgctggg cgtggtcggt agcgcctttc atctgttttg aagactcccc
     4321 tccttgaaaa ggaggggagt ttgttccccg cctctctcaa ggaagccaac ggatgtactt
     4381 ggatataggg cttctttttc gggcgcgccg gcccgcgcca gcgggctctt gatgctcacg
     4441 aggcggggtg gcgccggggc ggtgtggtgt gcgtgctcgt catcgcagga ccgttcatga
     4501 atagtccagg ctaaaggacc ttatccaatg gctgagctgt acgtcatcga ccttcccgcg
     4561 gttccctcca ttcccgtgac cggccagaag gcgcgcttcc cggtgcaccg catctattgc
     4621 gtcgggcgca actacgcggc gcacgcgcgc gagatgggca aagacccgga gaaggagccg
     4681 ccgttcttct tcatgaagcc ggccgacgcg gtgctgcccg agggcggcga gatgcactat
     4741 ccgccgggca ccgccaacct gcaccacgag atcgagctgg tggtggcatt gcaccgaggc
     4801 gggttcaaga tccccgcggc gcgcgcgctg gatcacgtgt acggctatgc cgtggggctg
     4861 gacatgacac ggcgcgatct gcagacgcag gcgcgcgaca tgggccgccc gtgggatttc
     4921 ggcaaggcgt tcgacgagtc ggcgccgatc agcgccatct acccggtggc cagccacggt
     4981 catcacagcg ccggcgcgat cgcgctcaag gtcaacggcg agctgcgcca gagcggcgat
     5041 ctgcaggaca tgatctggaa cgtccccgac accatcgcgt tcctgtccga gtactaccgc
     5101 ctgcagccgg gcgacctgat cttcaccggt acgccggccg gtgtgggcgc cgtgaagcag
     5161 ggcgacgtgc tggtgggcgc ggtcgagggg ctgacggagc tgcgggtgac gatagttccg
     5221 tgattcgtga ttggtgattc gtgaatcgtc gtgattcgta gtgagtcgcc gattttcgcg
     5281 aatcacgaat cgcgagtgac gaatcacgat tcacgaatca cgattcacgg atcacgaatc
     5341 acgaatcacg gatcaccaga agatgccaac ccccaagacc tggctggaag tctcgctgaa
     5401 tggcccctgg gggcgcgggc ggcagccgcg cattccggtg agcgtggacg agatcgtgcg
     5461 cgagggcgtc gcctgcgcgg aggcgggagc ggcgatcgtg cacgtgcacg cctacgacga
     5521 ggcgagcggc aagccgcagg agaacgccga gacctacgcg cgcatcatcg gcggcatccg
     5581 cgccgcgtgc gacgcgatcg tctacccgac catccccgtg ctcgggctgt cgcctttccc
     5641 gaaggaggct acggcggcgc agcgcttcgc gacggtcgaa gcgctggccg ggcgcggcct
     5701 gctggaatgg gcggcggtcg atcccggctc ggtgaacctg gcctattacg acgacttgcg
     5761 cgaggaccgc gagggcttcg tgtatctcaa tcccgaagac cacatccggc acgcgctcgg
     5821 gctcgcgcgc acgcaccgct tccaccccgc ctacgcgatc tacgagcccg gtttcctgcg
     5881 cctgggcgcg acgctgcact ggcgctgcag ctcgccggcg ccgctgtacc gcttcatgtt
     5941 ctcctccggg tacaccttcg gtttcccgcc cgacgactac ggcctcaccg cttatctcac
     6001 gctgctcgac caagtggcgc ccggggcgca atggatggtg ggcggcctgt cggtggacgt
     6061 gctgccgctg atcccgcgca tcgtggccga gggcggccat gtgcgcgtcg ggctcgagga
     6121 cgcgccgtgg ggctcccagc gcagcaacct ggactgggtg cgggaagcgg cgcgccgcat
     6181 cgacaacgcc ggcggcgagc tggccacgcc cgcggacgtg cgcgcggtga tgcgggcggt
     6241 ggagatggga gacgcgtgag gggcgtgcgg cacgtttgac gcatcaccga ggaaccagta
     6301 tggcggcatc ggcaaagatc ggcatcacca tgggcgaccc ctccggcatc ggaccggaga
     6361 tcatcgtcaa ggcgctgaag gaaatggcgc ccgagcaacg ctcggccacc gcggtgatcg
     6421 gcaacctcga catcctgaag cgcgccgaca ggctcgtcgg cgcgggtctg agttacgtgg
     6481 aaggcgtgga cgcggcgcgc g
//
LOCUS       DASPGE010000211         3537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1697248, whole genome shotgun sequence.
ACCESSION   DASPGE010000211 DASPGE010000000
VERSION     DASPGE010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697248"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="VD791_06765"
     CDS             <1..1019
                     /locus_tag="VD791_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07713.1"
                     /translation="GVQLPSLPGGLITRPTLLWDVSAKKAGEHLARVAYQTGGITWWA
                     DYNLTYAEGKDANSCKLDVGAWVSIVNQSGASYPDARIKLIAGDVHRAPVPGQMLAKR
                     AAPAAEAMMDEARGFEEKAFFEYHLYTLGRASSLPDNSTKQIELFPQVAAVPCEKTLV
                     YYGQQDFYWGYGGNPYTDRNYGITGNKKVDTYLAFQNIKENGMGVPMPAGRVRVSKLD
                     GADGSLEFIGEDRIDHTPRNEKILLKLGSAFDVVGERRQMDYSVDTARKVITEEIEVK
                     LRNRKEEPVQVIVKENLYRWVNWRIVSSTHAYQKQDARTIHIPVNVPADKEVVVRYTV
                     RYTW"
     gene            complement(1029..2567)
                     /locus_tag="VD791_06770"
     CDS             complement(1029..2567)
                     /locus_tag="VD791_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07714.1"
                     /translation="MFALTRNKSKKQTAAADALAATQLLVEGLPRDDPLAALRELAAQ
                     LDRVKADDEVRPFRAFEIVDLIDRVGRPHYRKAAHEYVLGQRRLTKFQETQLWTGVSA
                     YLFELSQGYRYCLAKYEVGADSIGSLTPLLPVICARALRIGAARLKWSYLRYRPLEPR
                     HWSELAALYFLAETNGFARQVLPLYRGAAQDSCVEHEFLKTLILAISSPTALLPEQIE
                     IAERLIVRSVGDFVISAAVTDKLQHYIDLRSDAGPQRLTAGEKLRFTARAFGVCRAVE
                     RLQAIDRGLEQGEMTPRELSLTQEFEIEVIRATIRHLLRQWRPPLPQRRHSRQRQSTP
                     LTVAHDPDEVTANIGGLSYPFVSEQETWLMDNRSDSGLHAVVMSPQGRWIEVGSLIAY
                     REGEDAIWSAGLVRRVAREDDDTRQVAVETIAHGGAAVTVLPLAAGQRAEPAEGVLCT
                     LLLGRNVASDQLTLLMPPKSFSPTAAREMRAYDRRYRLIPLRLIESWAGYEIARFKVL
                     QLAE"
     gene            2724..>3537
                     /locus_tag="VD791_06775"
     CDS             2724..>3537
                     /locus_tag="VD791_06775"
                     /inference="COORDINATES: protein motif:HMM:NF006094.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HXV07715.1"
                     /translation="MISSLDIEAAAARIAPHVRVTPVIALEPRAFGSPARLTLKLELM
                     QHTGSFKPRGAFNRILASRVPDSGVIAASGGNHGAAVAYAARQLDHVAEIFVPEPTPD
                     VKVERLRRYGARVVLAGANYAEAYAASGERARQTGALEVHAYDQAEVLAGQGTLAREL
                     ERQTEGLDTVLVGVGGGGLIGGMAAWFAGRVKVVAIEPERCPTLHAAVQAGAPVDVEV
                     GGIAADSLGARRVGGLMFEIAQRFVDAVVLVSDEEIRQSQRALWDSLRVLAEP"
BASE COUNT          574 a         1194 c         1243 g          526 t
ORIGIN      
        1 cgggcgtgca gctgccgagc ctgcccggcg gcctgatcac gcggcccacg ctgttgtggg
       61 acgtatccgc caagaaggcg ggcgagcacc tcgcgcgcgt ggcctatcag accggcggca
      121 tcacctggtg ggccgactac aacctcacct acgccgaggg caaggacgcc aattcctgca
      181 agctcgacgt cggcgcctgg gtgagcatcg tcaaccagtc cggcgcttcc tatcccgacg
      241 ccaggatcaa gctcatcgcc ggcgacgtgc accgggctcc ggttcccggg cagatgctcg
      301 ccaagcgcgc cgcaccggcc gcggaagcga tgatggacga ggccaggggc ttcgaagaga
      361 aagccttctt cgagtatcac ctgtacacgc tggggcgcgc cagctcgctg ccggacaact
      421 ccaccaagca gatcgaactg ttcccgcagg tcgctgcggt gccctgcgag aagacgctcg
      481 tgtactacgg gcagcaggat ttctactggg gctacggcgg caacccgtac accgaccgca
      541 actacggcat caccggcaac aagaaggtgg acacctacct cgcattccag aacatcaagg
      601 agaacggcat gggcgtaccg atgcccgccg gccgcgtgcg cgtgagcaag ctggacggcg
      661 ccgacggctc gctggaattc atcggcgagg accgcatcga tcacacgccg cgcaacgaga
      721 agatcctgct gaagctcggc tcggcgttcg acgtggtcgg cgagcgccgc cagatggact
      781 acagcgtgga cacggcgcgc aaggtgatca ccgaggagat cgaggtcaag ctgcgcaacc
      841 gcaaggaaga gccggtgcag gtgatcgtga aggagaatct gtaccgctgg gtcaactggc
      901 gcatcgtgtc gagcacgcac gcctatcaga agcaggacgc gcgcaccatt cacattccgg
      961 tgaacgtgcc ggcggacaag gaggtggtgg tgcgctacac ggtgcgctac acgtggtagc
     1021 gtccgtgctc actctgcgag ttgcagcacc ttgaagcggg cgatctcgta gcccgcccag
     1081 gactcgatca ggcgcagcgg gatcagccga tagcgccgat cgtaggcgcg catctcgcgc
     1141 gcggcagtcg gggagaagct tttcggcggc atcagcaggg tgagctggtc gctcgcgaca
     1201 ttgcgtccca ggagcagcgt gcacagcacg ccttcggcgg gctcggcgcg ctgccccgcg
     1261 gccaggggca acaccgtcac cgccgctccg ccgtgcgcga tcgtctcgac ggcgacctgg
     1321 cgcgtgtcgt cgtcctcgcg cgcgacgcgg cgcaccaggc cggcgctcca gatggcgtcc
     1381 tcgccttcgc ggtaggcgat caggctgccg acctcgatcc agcggccctg cgggctcatc
     1441 acgacggcgt gcaggccgct gtcgctgcga ttgtccatga gccaggtctc ctgctcgctc
     1501 acgaagggat aggacaggcc gccgatgttg gccgtcacct cgtccgggtc gtgcgccacc
     1561 gtgagcggcg tcgactgccg ctgccggctg tgccggcgct gcggcaacgg cggcctccac
     1621 tggcgcagca gatgccggat cgtggcccgg atgacctcga tctcgaactc ctgggtgaga
     1681 ctcagctcgc gcggggtcat ctcgccttgc tcgaggccgc ggtcgatcgc ctgcagccgt
     1741 tccaccgccc ggcacacgcc gaaggcgcgc gcggtgaagc gcagtttctc tcccgccgtc
     1801 agccgctgcg gtccggcgtc cgagcgcaaa tcgatatagt gctgcagctt gtcggtgacc
     1861 gctgcgctga tgacgaaatc gccgaccgag cgcacgatca ggcgctccgc gatctcgatc
     1921 tgctcgggca acagggccgt cggcgaggag atggcgagaa tcaacgtttt cagaaactcg
     1981 tgctcgacgc aggagtcctg cgccgcgccg cgatacagcg gcagcacctg gcgcgcgaaa
     2041 ccgttcgtct cggcgaggaa gtacagggcg gcgagctcgc tccaatgccg cggctccagg
     2101 gggcggtagc gcaggtacga ccatttcagg cgcgcagcgc cgatgcgcaa cgcgcgcgca
     2161 cagatgaccg gcagcagggg agtcaggctg cctatgctgt cggcgcccac ctcgtacttc
     2221 gccaggcagt agcgataccc ctgcgacagc tcgaacaggt aggcggagac gccggtccac
     2281 agctgcgtct cctggaattt agtgagccgg cgctggccca atacgtactc gtgggcggcc
     2341 ttgcgatagt gcggacggcc gacccggtcg atgagatcga cgatctcgaa agcgcggaac
     2401 gggcgcactt cgtcgtccgc cttgacgcgg tcgagctgcg ccgccagctc gcgcagcgcg
     2461 gcgagcgggt cgtcgcgcgg caggccctcc accagcaact gcgtggcggc cagcgcgtcc
     2521 gccgctgcgg tctgtttctt cgatttgttc cgggtgagtg cgaacatgcg cggccgatgt
     2581 gtgaaagatg tgacaaccgg gcgacaccac tcggcccgct tgggtggcgc cggccgggcc
     2641 gacgcccgaa tccgggcgta tcaagcaagg ggtgggccgg tgacggcccg actgggcgag
     2701 tcctttcttg tatcctctgg cccgtgatct cctccctcga catcgaagcg gcggccgcac
     2761 gcatcgcgcc gcacgtgcgg gtgaccccgg tgatcgcgct cgagccgcgc gcgttcggct
     2821 cgcccgcacg gctcacgctc aagctggagc tgatgcagca caccggctcc ttcaagccgc
     2881 gcggcgcgtt caaccgcatc ctggcgagcc gcgtgcccga cagcggcgtg atcgccgcct
     2941 ccggcggcaa tcacggcgcg gcggtggcgt acgcggcgcg ccagctcgac cacgtggcgg
     3001 agatcttcgt tcccgagccc acaccggacg tgaaggtcga gcgcctgcgc cgctacggcg
     3061 cgcgcgtggt cctggcgggc gcgaactacg ccgaagccta cgcggccagc ggcgagcgcg
     3121 ctcgccagac gggcgcgctg gaggtgcacg cctacgacca ggccgaagtc ctggccgggc
     3181 agggcacgct ggcgcgcgag ctggaacgtc agaccgaagg cctggacacg gtgctggtcg
     3241 gcgtgggcgg cggcggcttg atcggtggca tggcggcctg gttcgccggc cgcgtcaaag
     3301 tggtcgccat cgagcctgaa cgctgcccga cgctgcatgc cgcggtccag gcgggcgcgc
     3361 cggtggacgt agaggtgggc ggcatcgccg cggactcgct cggtgcgcgc cgcgtcggcg
     3421 gcctgatgtt cgagatcgcg caacggttcg tggacgcggt ggtgctggtg agcgacgaag
     3481 agatccgcca gagccagcgc gcgctgtggg acagcttgcg cgtgctggcc gagcccg
//
LOCUS       DASPGE010000212         3270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_877923, whole genome shotgun sequence.
ACCESSION   DASPGE010000212 DASPGE010000000
VERSION     DASPGE010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877923"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VD791_06780"
     CDS             <1..395
                     /locus_tag="VD791_06780"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HXV07716.1"
                     /translation="SVVFTGQLLTRLHFPLELDYLHVTRYDGATRGGTLSWKVFPRQP
                     VAGRPVLVLDDILDEGTTMAAIRDRLLAEGAQSVLIAVLADKAIGRRKPVTPDFVGLT
                     VPNRYVFGFGMDVEGMWRNLPAIYALRE"
     gene            400..1146
                     /locus_tag="VD791_06785"
     CDS             400..1146
                     /locus_tag="VD791_06785"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: protein motif:HMM:NF006599.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HXV07717.1"
                     /translation="MLAIIGGTGLTQLAGLEVTGRQVVRTPWGEPSGALTFGRIGGQA
                     VIFLARHGYGHTIPPHMVNYRANIWALREQGVTHVMSVASVGGIRQDLAPGVLALPHQ
                     IIDYTWGRKSTFFEGDERGVVHADFTHPYCQSLRQRCLEAAQRAAEPLIEGGVYACSQ
                     GPRLETAAEIDRLERDGADMVGMTGMPEAALAREIDLCYATVAVVANYAAGRAGSSAG
                     IRMDEINATLQTALAKVRRVIEQLAQSYAR"
     gene            1136..1669
                     /gene="def"
                     /locus_tag="VD791_06790"
     CDS             1136..1669
                     /gene="def"
                     /locus_tag="VD791_06790"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXV07718.1"
                     /translation="MPARPVLRMGDPRLLERSQEVERFDTPELHALIADMQDTMAAMS
                     GVGLAAPQIGVPLRVVIFGMEHNPRYPDAPPVPYTVLVNPLLTPLDDTVEEGWEGCLS
                     VPGMRGLVPRFARLRYQGRDQHGAPIDRSVIGFHARVVQHECDHLDGILYPMRIRDLR
                     NFGFLDVLFPGRPDTDE"
     gene            1803..2135
                     /locus_tag="VD791_06795"
     CDS             1803..2135
                     /locus_tag="VD791_06795"
                     /inference="COORDINATES: protein motif:HMM:NF017237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HXV07719.1"
                     /translation="MDSGVDPGVDSGAVLALYEKTLARTREMLDAARAGDWDGLVQRE
                     RSRALLVERLKEHDPDPARDAATLERKREILLEISRADEQIHTLTQDWMHELRDVLGG
                     LGTLQRLT"
     gene            2255..2635
                     /locus_tag="VD791_06800"
     CDS             2255..2635
                     /locus_tag="VD791_06800"
                     /inference="COORDINATES: protein motif:HMM:NF022428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HXV07720.1"
                     /translation="MEALVITWRELLVVVVAVLGVHAAALALLLRWSAGGARRKRSAQ
                     PAADSQALNGMNLEIAELRKQVGRLQSEVEKLRSLGLPASSPYNQAIQMARQGLSASE
                     VASGCGISRGEAELIVALYRKLRI"
     gene            complement(2707..>3270)
                     /locus_tag="VD791_06805"
     CDS             complement(2707..>3270)
                     /locus_tag="VD791_06805"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HXV07721.1"
                     /translation="LSPVGEGVQVLIYTPDQEALFARICAFFERISFSIVEAKVYTTL
                     HGYALDTFQVMDPARAGGHYRDILAYIEHELAAQLHRGGPLPPPVKGRISRRVRSFPM
                     TPEVSLRPDERGNAYYLFFTAGDRPGLLSSVARVLVEYGINVQTAKINTLGERAEDSF
                     VITGDALRETRNIINLESDLMRVLQTQ"
BASE COUNT          490 a         1123 c         1160 g          497 t
ORIGIN      
        1 gcagcgtcgt gttcaccggc cagctcctga cgcggctgca tttcccgctc gagctcgatt
       61 acctgcacgt cacccgctac gacggggcca cccgcggggg cacgctgtcc tggaaggtgt
      121 tcccgcgcca gccggtagcg gggcggccgg tcctggtgct tgacgacatc ctcgacgaag
      181 gcaccaccat ggccgccatc cgcgaccgcc tgctcgccga gggcgcgcag tcggtgctca
      241 tcgctgtgct cgccgacaag gcgatcggcc gccgcaagcc ggtcacgccg gacttcgtcg
      301 gcctgacggt gcccaaccgc tacgtgttcg gcttcggcat ggacgtggag ggcatgtggc
      361 gcaacttgcc ggcgatctac gcactgcgtg aatgacgtca tgcttgcaat catcggcggt
      421 acgggcctga cgcagctcgc cggcttggag gtcaccggcc gccaggtggt gcgcaccccc
      481 tggggcgaac cttcgggggc gctcaccttc ggtcgcatcg gcgggcaggc agtgatcttc
      541 ctggcacggc acgggtatgg gcacacgatt ccgccgcaca tggtgaacta ccgcgcgaac
      601 atctgggctc tgcgcgagca gggcgtgacg cacgtgatgt cggtggcgag cgtgggcggg
      661 atccgccagg acctcgcgcc cggtgtgctg gcgctgccgc accagatcat cgactacacc
      721 tggggccgca agtccacgtt cttcgaaggc gacgaacgcg gcgtggtgca tgccgacttc
      781 acgcatccgt actgccagag cctgcgccag cgctgcctgg aggccgcgca gcgcgccgca
      841 gagccgctga tcgagggcgg ggtctacgcc tgcagccagg ggccgcgcct ggagaccgcc
      901 gccgagatcg accggttgga gcgcgacggc gccgacatgg tgggcatgac cggcatgccc
      961 gaggccgcgc tcgcgcgcga gatcgatctg tgctacgcca ctgtggccgt ggtggcgaat
     1021 tatgccgcag ggcgcgcggg cagcagtgca ggcatccgca tggacgagat caacgccacg
     1081 ctgcagaccg cgctcgccaa ggtgcgccgc gtcatcgagc agctggcgca gagctatgcc
     1141 cgctaggccg gtgctgcgca tgggagatcc gcgcctgctg gaacgctcgc aggaggtgga
     1201 gcgcttcgac acgccggagc tgcacgcgct gatcgcggat atgcaggaca ccatggcggc
     1261 gatgagcggc gtgggcctcg ccgcgccgca gatcggcgtc ccgttgcgcg tggtgatctt
     1321 cggcatggag cacaatccgc gctatcccga cgcgccgccg gtgccctaca cggtgctggt
     1381 caatccgctg ctcacgccgc tcgacgacac ggtggaggag ggctgggaag gctgcctgag
     1441 cgtgcccggc atgcgcggac tggtgccgcg cttcgcgcgc ctgcgctacc agggccgcga
     1501 tcagcacggc gcgccgatcg accgcagcgt gatcggcttc cacgcgcgcg tggtgcagca
     1561 cgagtgcgat catctggacg ggatcctcta cccgatgcgc atccgcgacc tgcgcaactt
     1621 cggcttcctc gacgtgctgt tccccgggcg tcccgatacc gacgagtagt cgtgcctcgc
     1681 ggccggctca agcccgccgg gcgctcgccg atagtgtctg gcgggcagcc gatcacagga
     1741 ggcactggat ggccctgtct tcaccgggcg tgggacccgg gctggactga ggacccggcg
     1801 gcgtggattc cggcgtcgat cccggcgtcg attctggggc cgtgctcgcg ttgtacgaga
     1861 agacgctcgc gcgcacgcgt gagatgctcg acgcggcgcg cgcgggcgac tgggatggcc
     1921 tggtccagcg cgaacgcagc cgcgccctgc tggtcgagcg cctgaaagag cacgaccccg
     1981 atccggcgcg cgacgctgcg acgctggagc gcaagcgcga gatcctcctg gagatctccc
     2041 gcgccgacga gcagatccac actctgacgc aggactggat gcacgagctg cgcgacgtgc
     2101 tcggcggcct cggcaccctg cagcgcctga cgtgagcgca tccgcgtgag agccgaccgt
     2161 caccgccgtg cgccgatcgt gttgacgcct tctgcccaaa gtagcaaact cagcccccgg
     2221 agtcagcgca gcgcatcggt tcggggagca ggtcgtggag gccctggtaa tcacctggcg
     2281 ggagttgctg gtcgtggtcg tcgccgtcct cggcgtgcac gccgccgcgc tggcgctgct
     2341 gctgcgctgg tcggccggtg gcgcgcgccg gaaacgcagc gcgcagcccg ccgcggacag
     2401 ccaggcgctc aacggcatga acctcgagat cgccgagctg cgcaagcagg tgggccgcct
     2461 gcagtcggag gtggagaagc tcagatcgct cggcttgccg gcgtcctcgc cgtacaacca
     2521 ggcgatccag atggcgcggc agggcctgag cgcctcggag gtcgccagcg gctgcggcat
     2581 ctcgcgcggc gaggcggagc tgatcgtggc gctgtaccgc aagctgcgca tctaggctag
     2641 ggcgccatag aagccgagcc gccgccctaa gcctgcgcaa gcggcggcgc ccgccgccgg
     2701 ggcgggtcac tgggtctgca gcacccgcat cagatcgctc tcgagattga tgatgttgcg
     2761 cgtctcgcgc agcgcatcgc cggtgatcac gaagctgtct tcggcacgct cgcccagagt
     2821 gttgatcttc gccgtctgca cgttgatgcc gtactccacc agcacccgcg ccacgctcga
     2881 caacagcccg gggcggtcgc cagccgtgaa gaacagatag taggcgttgc cgcgctcgtc
     2941 cgggcgcagg ctcacttccg gcgtcatcgg gaagctgcgc acgcgccggc tgatgcgtcc
     3001 cttcaccggc ggcggcagcg gcccgccgcg gtgcagctga gcagccagct cgtgctcgat
     3061 gtaggcgagg atgtcgcgat agtgcccgcc ggcgcgcgcg gggtccatca cctggaacgt
     3121 atccagcgca tagccgtgca gcgtggtgta caccttggcc tcgacgatgc tgaagctgat
     3181 gcgctcgaag aacgcgcaga tgcgcgcgaa cagggcctcc tggtccgggg tgtagatcag
     3241 cacctgcacc ccttcgccca ccgggctgag
//
LOCUS       DASPGE010000213         3807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_409416, whole genome shotgun sequence.
ACCESSION   DASPGE010000213 DASPGE010000000
VERSION     DASPGE010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409416"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VD791_06810"
     CDS             complement(<1..715)
                     /locus_tag="VD791_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXV07722.1"
                     /translation="MNHEAADASQLLDCLSGRLPALRQALLADREAQLDRVELRAGAR
                     LLSKGEVATAVYVTASGLLRATAVREDGSELTLSEFGAGELAGEMAILTGGGVYSASV
                     SAAQDSVLVRVPRKTFERIAGTSEQVIQEMSEGVRRRIARDQLVLGLTRLFGPLHESV
                     LRYVETRVQWVRLQSGETLFSAGDSGRDLYFIVSGRLRALGPGGRVLNEMTRGESIGE
                     IALLTGEPRSATVVAVRDSA"
     gene            complement(781..2940)
                     /gene="aas"
                     /locus_tag="VD791_06815"
     CDS             complement(781..2940)
                     /gene="aas"
                     /locus_tag="VD791_06815"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HXV07723.1"
                     /translation="MRRRRALLKPLLRALLKLLFRVRVEGDAGHLSAPRLLIIANHQS
                     FLDGVILGAFLPVDAVFVVHTDVTRNALFRLVLSLSDYLAVDPTNPMAMKRVVRLIES
                     GRPVVIFPEGRITLTGSLMKVYDGPAFVAARTNAVVAPVRLDGATRSYFSRLSGSHPR
                     ALLPRITVSLQPPTAVAMPVGESGRMRRRKAGESMRRIMQDMIFRSQPIQTLYDALID
                     AMRVYGRRRKLVEDMKQTEYSYNALLKMTLVLGLLVRRQSARGETVGILLPNLAATLC
                     MVIGTTAFRRIPAMLNYTAGTEGMQSACVAARIATVFTSRKFLETARLQAQVDALRGV
                     RIVFLEDLQPTVTLRVKLWALLHLFLPRLLGRRVNPDSPAVVLFTSGSEGRPKGVVLS
                     HRAILSNIAQVRAITDFTIEDRIFNALPIFHSFGLTAGGLLPILSGTQLFLYPSPLHY
                     RLIPEIVYNRSCTVLFGTSTFLGNYAKFAHPYDFYRVRYVVAGAEKLSQAVRELWFDK
                     FGSRIFEGYGVTETAPVVSVNCPMATRTGSVGQLLPGMEAKLVPVPGIERGGVLHLRG
                     PNVMLGYLHYANPGVLEPPKSELGPGWYDTGDIVDIDADGFLHILGRVKRFAKIAGEM
                     ISLEVVEKIAVAASPGFQHAASSRPDSQRGEALVLFTTDPALSRERLQQAAQTLGLPE
                     IAVPRRLHAVSAIPLLGTGKVNYAKLKEDALALATAP"
     gene            complement(2952..>3807)
                     /locus_tag="VD791_06820"
     CDS             complement(2952..>3807)
                     /locus_tag="VD791_06820"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HXV07724.1"
                     /translation="DAELVKLDEMAARQVDDPMLRFGGEDESATLDPKVNTNVVGGNP
                     VAAAELGLRNIDRVVPMLIPATTKLGGSYEPLREMWDALITQRHRELNAVAKVVGGVE
                     ETRYQAGRGRAPFQPVPAQEQRAAVKFLLTYAFSTPRKMLDPEVMLRITPTGATDGLQ
                     DSNKRLLERIIEAGVFQRMAEAQALAPKAKGYSGLDLLYDLNDGLFSELNAERPSIEL
                     YRRELQRTYVALILERVEGQPSEFRAALRLGAGDLLEKINKALKKTKDIVSAAHLYDL
                     SVELGQVQ"
BASE COUNT          592 a         1324 c         1289 g          602 t
ORIGIN      
        1 gcgcactgtc gcgcacggcc accacggtcg cactgcgcgg ctcgccggtg agcagcgcga
       61 tctcgccgat gctctcaccg cgggtcatct cgttgagcac gcggccgccc gggcccaggg
      121 cgcgcagcct gccgctcacg atgaaataca gatcccgccc gctgtcgccc gccgaaaaca
      181 gcgtctcgcc cgactgcagc cgcacccact gcacacgcgt ctccacatag cgcaagaccg
      241 attcgtgcag cgggccgaac aggcgcgtga ggccgagcac gagctggtcg cgtgcgatgc
      301 gccgccgcac gccctcggac atttcctgga tgacctgctc ggaagtgccg gcgatgcgct
      361 cgaaggtctt gcgcggcacg cgcaccagga cgctgtcctg cgccgccgag accgaggcgc
      421 tgtacacgcc gccgccggtc aggatcgcca tctcgccggc cagttctccg gcgccgaact
      481 ccgacaaagt gagctcgctg ccgtcttcgc gaacggcagt ggcgcgcagc agcccgctgg
      541 cggtcacgta gacggcggtc gccacctcgc ccttgctgag gagccttgcc ccggcgcgca
      601 gctccacccg gtcgagctgc gcctcgcggt ccgcgagcag cgcctggcgc agcgccggca
      661 ggcgcccgga caggcagtcg aggagctgcg aggcgtcggc ggcttcgtgg ttcatgtctg
      721 tgcctgcagt gttcgatgca tccggcgcgg gcgcttcagc gccggcaatt gggcctgtcg
      781 tcaaggcgcc gtcgccaggg ccaacgcgtc ctccttgagc ttggcgtagt tcaccttgcc
      841 ggttccaagc agcgggatgg cggacacggc gtgcagcctc cgcggcacgg cgatctccgg
      901 caagccgagc gtctgcgccg cctgctgcag gcgctcgcgg ctgagcgccg ggtcggtggt
      961 gaacagcacc agcgcctcgc cgcgctggct gtccgggcgc gaggaggccg cgtgctggaa
     1021 gccgggcgag gcggccacgg cgatcttctc caccacctcg agcgagatca tctcgccggc
     1081 gatcttcgcg aaacgcttca cgcgcccgag gatgtgcagg aagccgtccg cgtcgatatc
     1141 cacgatatcg ccggtatcgt accagcccgg ccccagctcc gatttcggcg gctcgagcac
     1201 gccggggttc gcgtagtgca ggtagcccag catgacgttg gggccgcgca ggtgcagcac
     1261 gccgccgcgc tcgatgccgg gcaccggtac cagcttcgcc tccatcccgg gcagcagttg
     1321 cccgacgctt ccggtgcgcg tcgccatcgg gcagttcacc gacactaccg gtgcggtctc
     1381 ggtgacgccg tagccctcga agatgcggct gccgaacttg tcgaaccaca gctcgcgcac
     1441 cgcctgggag agtttttccg cgcccgccac gacgtagcgt acgcgataga agtcgtacgg
     1501 atgggcgaac ttggcgtagt tgccgaggaa ggtgctggtg ccgaacagga cggtgcagct
     1561 gcggttgtac acgatctccg ggatcaggcg gtagtgcagc ggcgaggggt agagaaacag
     1621 ctgggtgccg ctcaggatcg gcagcaagcc ccccgccgtg aggccgaagg aatggaagat
     1681 cggcagggcg ttgaagatcc ggtcctcgat cgtgaagtcg gtgatcgccc gtacctgcgc
     1741 gatgttggac agaatcgccc ggtgcgacag caccacaccc ttgggccggc cctcggagcc
     1801 ggaggtgaac aacaccaccg ccggcgagtc ggggttcacg cggcgcccca gcaggcgggg
     1861 caggaacagg tgcagcaggg cccacagctt cacccgcagc gtcaccgtcg gctgcaggtc
     1921 ctcgagaaag acgatccgca cgcccctcag cgcatccact tgcgcctgca gccgggccgt
     1981 ttccaggaac ttgcgcgagg tgaagacggt agcgatgcgc gccgccacgc acgcgctttg
     2041 catgccctcg gtgccggccg tgtagttgag catggcgggg atgcgccgga acgcggtcgt
     2101 gccgatcacc atgcacagcg tggccgcgag gttgggcagc aggatgccca cggtctcgcc
     2161 gcgcgcgctc tggcggcgca ccagcagccc cagcaccagg gtcatcttga gcagcgcgtt
     2221 gtaggagtat tcggtctgct tcatgtcctc gaccagcttg cgcctgcgcc cgtacacgcg
     2281 catcgcgtcg atcagcgcgt cgtacagcgt ctggatcggt tgcgagcgga agatcatgtc
     2341 ctgcatgatc cggcgcatgc tttcgccggc cttgcggcgg cgcatacggc cgctctcgcc
     2401 gaccggcatg gcgaccgcgg tgggcggctg cagcgagacc gtgatgcgcg gcagcagggc
     2461 gcgcgggtgg ctgcccgaca gccggctgaa atagctgcgc gtggcgccgt ccagccgcac
     2521 cggcgcgacc acggcgttgg tgcgggcggc gacgaaggcc ggaccgtcgt acaccttcat
     2581 caggctgccg gtgagcgtga tgcgtccttc cgggaagatc accaccggcc gccccgactc
     2641 gatcagccgc accacgcgct tcatcgccat cgggttggtg ggatccaccg ccaggtagtc
     2701 gctcagcgag agcaccagac ggaacagcgc attgcgggtc acgtcggtgt gcaccacgaa
     2761 caccgcatcc accggcagga acgcccccag gatcacgccg tccaggaaag actgatggtt
     2821 ggcgatgatc agcagccgcg gcgcggacaa gtgaccggcg tctccctcca cgcgcacgcg
     2881 gaacagcagc ttgagcagcg cgcgcagcag aggcttaagc agggcgcgtc gtcgtcgcat
     2941 ggcgctcgtc cctactgcac ctgacccagc tcgacgctga gatcgtacag gtgcgccgcg
     3001 ctgactatgt ccttggtttt cttcagcgcc ttgttgatct tctccaggag gtcgcccgcg
     3061 ccgagccgca gcgcggcgcg aaactcgctg ggctggccct ccacgcgctc caggatcagc
     3121 gcgacatacg tgcgctgcag ctcgcggcgg tacagctcga tgctcggccg ctccgcgttc
     3181 agctcggaga acaatccgtc gttgaggtcg tagagcagat ccaatccgga gtagcctttg
     3241 gccttgggag cgagcgcctg cgcctccgcc atgcgctgga acacgccggc ctcgataatc
     3301 cgctccagca gcctcttgtt ggaatcctgc agcccgtccg tggcgccggt cggtgtgatg
     3361 cgcagcatca cctcggggtc gagcattttc cgcggcgtgc tgaaggcgta cgtcagcagg
     3421 aatttcaccg cggcgcgctg ctcctgcgcg ggcaccggct ggaaaggcgc cctgccgcgc
     3481 ccggcctggt agcgcgtttc ctccacgccg cccactacct tggccacggc gttgagctcg
     3541 cgatggcgct gggtgatcag cgcatcccac atctcgcgca gcggctcgta gcttccgccc
     3601 aatttggtgg tggctggaat cagcatcggc accacacggt cgatgttgcg caaccccagc
     3661 tcggccgccg ccaccgggtt gccgccgacc acgttggtgt tgaccttggg gtcgagcgtg
     3721 gcggattcgt cctcgccgcc gaagcgcagc atcggatcgt cgacttggcg cgcggccatc
     3781 tcgtccagct tgacgagctc ggcgtcg
//
LOCUS       DASPGE010000214         3150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1462277, whole genome shotgun sequence.
ACCESSION   DASPGE010000214 DASPGE010000000
VERSION     DASPGE010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462277"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..713
                     /locus_tag="VD791_06825"
     CDS             51..713
                     /locus_tag="VD791_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HXV07725.1"
                     /translation="MSQQRRKLLAGGAAALALATPLARAAAALVTPAQGKGPFYPLEL
                     PLDQDNNLVQVAGVSGTAQGTIIDVFGQLRDEQGRPLAGVALEIWQVNAFGRYHHPHD
                     QQDKPWDPSFQGFGRTRTDAQGAYRFRTVRPVAYPGRAPHIHFALSDSRFATFYTQMY
                     LAGAPENERDFLLASVRDRRARESLIVNLEPASPAEGAQGAKLAGRFDIVLGETVLSR
                     KP"
     gene            763..975
                     /locus_tag="VD791_06830"
     CDS             763..975
                     /locus_tag="VD791_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003276168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HXV07726.1"
                     /translation="MELTEAEVQSIKERIAQLRIEHRDLDDVITRLMTDPAHDELQMR
                     RLKRRKLLLKDQIALLERQINPDIPA"
     gene            complement(992..1297)
                     /locus_tag="VD791_06835"
     CDS             complement(992..1297)
                     /locus_tag="VD791_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HXV07727.1"
                     /translation="MIRLASICAGALAVLATAPAAAQDLERGRLLHENHCRMCHDSIA
                     YKRGAKLAADYTGVKAQVTRWQTNTGLRWSAEDIDNVTAFVAQTYYKYPLPGETKRD"
     gene            1517..2272
                     /locus_tag="VD791_06840"
     CDS             1517..2272
                     /locus_tag="VD791_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07728.1"
                     /translation="MQVTAGHPFRATLVLGLTALLLGACATHTPMPIDEVVQRSRQGQ
                     TADQIVGELRGSRTTFALRGSDFGRLAQAGVADTVLDHLQQSFVNDVDMQTRYWATGE
                     SLGGCSSCYPQQVDLSGLDSGGPAQQRPPSTYYTFSQPAGMPDWYRPYSAKRRTISLD
                     DVRRMAQQGTSTEEMVRAVRSARLDRTIGVAGMGTVRTHPVAGLSGSELARLRAEGIP
                     DPVLDEIQNAFVSQFVELQRLRYQHLGKGPSFS"
     gene            complement(2274..>3150)
                     /locus_tag="VD791_06845"
     CDS             complement(2274..>3150)
                     /locus_tag="VD791_06845"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXV07729.1"
                     /translation="GSTNAVLHYLAIASAAEVEWTIDDFERIRQKVPVLCNLKPSGKY
                     VAVDFHRAGGVPQVLKMLLKHGLLHGDCPTITGRTIAEELKDVPDAPRKDQDVIRQWD
                     DPLYTQGHLAILRGNLAPEGCVAKITGLKSPAITGPARVFDSENDTMEAIMGGKIRAG
                     DVVVIRYEGPRGGPGMQEMLAPTAALIGQGLGESVGLITDGRFSGGTWGMVVGHVAPE
                     AFVGGPIALVKEGDSITIDAHKRLIQLNVAEDELARRRAQWKPPAARYTRGLMAKYTR
                     LVSTASKGAVTDRGE"
BASE COUNT          493 a         1112 c         1071 g          474 t
ORIGIN      
        1 ccatgctgaa ggcgctcgag atctaccaga ggtactagca ggagccgacc atgtcacagc
       61 agcgcaggaa gttgctggcc ggcggagcgg ccgcgctggc gctggccacg ccgctggcgc
      121 gcgcggccgc cgcgctcgtc accccggcgc agggcaaggg gccgttctac ccgctcgagc
      181 tgccgctcga ccaggacaac aacctggtgc aggtggccgg ggtcagcggc acggcccagg
      241 gcaccatcat cgatgtgttc ggccagttgc gcgacgagca gggccgcccg ctggcgggcg
      301 tcgcgctcga gatctggcag gtcaacgcct tcggccgcta ccaccatccg cacgaccagc
      361 aggacaagcc ctgggacccg agcttccagg gcttcgggcg cacgcgcacc gacgcgcagg
      421 gcgcctaccg cttccgcacg gtgcggccgg tggcctaccc gggccgtgcg ccgcacatcc
      481 atttcgcgct gagcgacagc cgcttcgcga ccttctatac tcaaatgtac ctcgcgggcg
      541 ccccggagaa cgagcgcgac ttcctgctcg ccagcgtgcg cgatcgcagg gcgcgcgaga
      601 gcctgatcgt caacctcgag cccgcatccc ccgccgaggg ggcgcagggc gcgaagctgg
      661 ccggccgctt cgacatcgtg ctgggcgaga cggtgctgtc gcgcaagcct tgatggttct
      721 tccattcaac ctgctggagg agagcaagcg atggaggaga cgatggaact gacggaggca
      781 gaagtgcagt cgatcaagga gcgcatcgcg cagttgcgca tcgagcaccg tgacctcgac
      841 gacgtcatca cgcgcctgat gaccgacccg gcgcacgacg agctgcagat gcgccgtctg
      901 aaacgccgca agctgctgct gaaggatcag atcgcgctgc tcgagcgcca gatcaacccg
      961 gacatccccg cctgagccgg cgcggggtgc gctagtcgcg cttggtttcg ccgggcagcg
     1021 ggtacttgta gtaggtttgc gccacgaacg ccgtcacgtt gtcgatgtcc tccgcgctcc
     1081 agcgcagccc ggtgttggtc tgccagcgcg tgacttgcgc cttgaccccg gtgtagtcgg
     1141 cggcgagctt ggcaccgcgc ttgtaggcga tcgagtcgtg gcacatgcgg caatggttct
     1201 cgtgcagcag gcggccgcgc tcgagatcct gagcggcggc aggggcggtc gccaggacgg
     1261 cgagcgcgcc ggcgcaaatc gatgcaagtc tgatcatgaa ggtctccgca acgcactcac
     1321 actaacgtcg atcttgcctg cgcggttgat ccacgtcaaa gacgccgccc cgccgggcga
     1381 cgtcaaaata gcgaaccgcc cgatggcccc ggcgcgcccg gcgtcgaact cccgggtgtc
     1441 gaactcccgg gcgccgaact cccggccgct cgtggggtcg gatacgcgtc ggcaactcca
     1501 tccaggagca cccaccatgc aagtcacagc cgggcacccg ttccgtgcaa ccctcgtact
     1561 cgggctgacc gccctgctgc tcggcgcgtg cgcaacgcac acgccgatgc ccatcgacga
     1621 ggtggtgcag cgcagccggc aggggcagac ggccgatcag atcgtcgggg agctgcgcgg
     1681 ctcgcgcacg acgttcgcgc tgcgcggctc ggacttcggc cggctggcgc aggccggggt
     1741 ggccgacacc gtgctcgacc atctgcagca atccttcgtc aacgacgtgg acatgcagac
     1801 gcgctactgg gcgacgggcg agtccctcgg cggctgcagc agctgctatc cgcagcaggt
     1861 ggacctgagc gggctggaca gtggcggccc cgcgcagcag cgcccgccga gcacctacta
     1921 cacgttctcg caaccggccg gcatgcccga ctggtaccgg ccctacagcg cgaagcgccg
     1981 cacgatctcg ctcgacgacg tgcgccgcat ggcgcagcag ggcacgtcca ccgaggagat
     2041 ggtgcgcgcg gtgcgcagcg cgcggctgga tcgcaccatc ggcgtggccg gcatgggcac
     2101 cgtccgcacg cacccggtgg ccggcctgag cgggtcggag ctcgcgcggc tgcgcgcaga
     2161 gggtataccg gacccggtgc tcgacgagat ccagaacgcg ttcgtcagcc agttcgtgga
     2221 gctgcagcgc ctgcgctacc agcatctcgg caaggggccc agcttctcct gatctactcg
     2281 ccgcggtcgg tgaccgcacc cttgctcgcg gtggaaacca gccgggtgta tttcgccatc
     2341 agcccgcgcg tgtagcgcgc cgcgggcggc ttccactgcg cgcggcggcg ggccagctcg
     2401 tcctcggcca cgttgagctg gatcaggcgc ttgtgcgcat cgatggtgat ggaatcgccc
     2461 tccttcacca gcgcgatcgg cccgccgacg aacgcttccg gcgcgacgtg accgaccacc
     2521 atgccccagg tgccgccgga gaagcgcccg tcggtgatca gccccaccga ctcgcccagc
     2581 ccctgcccga tcagcgccgc ggtgggggcc agcatctcct gcatgcccgg accgccccgc
     2641 gggccttcgt agcggatcac caccacgtcg ccggcgcgga tcttgccccc catgatcgct
     2701 tccatggtgt cgttctcgga gtcgaacacc cgcgccgggc cggtgatcgc cgggctcttg
     2761 agcccggtga tcttggccac gcagccctcc ggcgccagat tgccgcgcag gatggcgaga
     2821 tggccctggg tgtacaaagg atcgtcccat tggcggatca catcctgatc cttgcgcggc
     2881 gcatccggga cgtccttcag ctcctcggcg atggtcctgc cggtgatggt cgggcagtcg
     2941 ccgtgcagca gcccgtgctt gagcagcatc ttgagcacct gcggcacgcc gccggcgcgg
     3001 tggaaatcga ctgccacgta cttgcccgag ggcttcaggt tgcacagcac cggcaccttc
     3061 tggcggatgc gctcgaagtc gtcgatggtc cattccacct cggcggccga agcgatggcg
     3121 aggtagtgca gcaccgcgtt ggtcgagccg
//
LOCUS       DASPGE010000215         5566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_761012, whole genome shotgun sequence.
ACCESSION   DASPGE010000215 DASPGE010000000
VERSION     DASPGE010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761012"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VD791_06850"
     CDS             <1..186
                     /locus_tag="VD791_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07730.1"
                     /translation="RPIQARGFNNYQHTLFKEFFCYGGVASGGPLGSAETIVRNLKHP
                     APQDNTVPRKFNQFQQR"
     gene            complement(241..666)
                     /locus_tag="VD791_06855"
     CDS             complement(241..666)
                     /locus_tag="VD791_06855"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HXV07731.1"
                     /translation="MRTPVIRPLLLCTALLMPLSASADALREGLWEVTVRMEIGGQPA
                     TAEPLVMRQCINQQSAQDLVAKLTGAGGGCQVSDLRQEGNRARWNMTCTGQIELTGTG
                     DVTITGDGFDGTLNATIGMGGTPVPILQTFGARRVGDCQ"
     gene            complement(799..1917)
                     /gene="proB"
                     /locus_tag="VD791_06860"
     CDS             complement(799..1917)
                     /gene="proB"
                     /locus_tag="VD791_06860"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HXV07732.1"
                     /translation="MPSVLANAKRLVVKVGSSLVTGQGQGLDHEALAQWAEQIAELKR
                     RGRQVLLVSSGAVAEGMQRLGWHKRPHALRELQAAAAVGQMGLVQAYESCFRNHGLHA
                     AQILLTHEDLSDRKRYLNARSTLHTLLQLDVIPVINENDTVANDEIKFGDNDTLGALV
                     TNLVEAEALVILTDQAGLYTRDPRRHPDATLVTEAKAGDPALEQMAGGVGSHVGSGGM
                     LTKVGAAKRAARSGAHTIIASGREPRVLLRLVDGEMIGTQLVAETMTLAARKQWLADH
                     LQVRGKLMLDAGAVRALTRNGKSLLPIGVQDLAGDFERGEVVSCLDPDGHEVARGLVN
                     YSAQDARRILRVPSHEIEARLGYIDAPELIHRDNLVLL"
     gene            complement(2025..3098)
                     /gene="obgE"
                     /locus_tag="VD791_06865"
                     /pseudo
     CDS             complement(2025..3098)
                     /gene="obgE"
                     /locus_tag="VD791_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
     gene            complement(3160..3423)
                     /gene="rpmA"
                     /locus_tag="VD791_06870"
     CDS             complement(3160..3423)
                     /gene="rpmA"
                     /locus_tag="VD791_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HXV07733.1"
                     /translation="MAHKKAGGSSRNGRDSNAKRLGVKRFGGQRVPAGSIIVRQRGTQ
                     FHPGANVGVGRDHTLFAKIDGIVRFAVKGPLGKQTVMVEPPSA"
     gene            complement(3436..3750)
                     /gene="rplU"
                     /locus_tag="VD791_06875"
     CDS             complement(3436..3750)
                     /gene="rplU"
                     /locus_tag="VD791_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HXV07734.1"
                     /translation="MYAVIKTGGKQYRVSAGEKLKVESLPADVGAEITLDQVLMVADG
                     DNVAMGTPTLSGASVKATVLGHGRGDKVKIFKMRRRKHYRKTQGHRQNYTEIQISGIS
                     LR"
     gene            3958..4935
                     /gene="ispB"
                     /locus_tag="VD791_06880"
     CDS             3958..4935
                     /gene="ispB"
                     /locus_tag="VD791_06880"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HXV07735.1"
                     /translation="MTIATLEPVHRLIAEEMRAVDEVIRVRLHSDVVLVRQVAEHIVG
                     SGGKRLRPALVILAAGACGYSGSAHYTLAAVVEFIHTATLLHDDVVDASQLRRGKATA
                     NSLFGNPASVLVGDFLYSRAFQMMVDLKDMRVMEVLANATNVIAEGEVLQLLNCHDAE
                     VTEHDYLHVIRCKTAKLFEAAGQLGAIVSNASPEVEQGLARYGMHVGTAFQLIDDALD
                     YSGDHAQTGKNLGDDLAEGKPTLPLIHAMQRGSAEQSRIIRAAIEHGGLDEFAPVLAA
                     VRETGALEYTRRQADAEAKRAVAALEALPRSSYRESLLQLASFAVSRSY"
     gene            4964..5040
                     /locus_tag="VD791_06885"
     tRNA            4964..5040
                     /locus_tag="VD791_06885"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4998..5000,aa:Pro,seq:cgg)
     gene            complement(5121..>5566)
                     /locus_tag="VD791_06890"
     CDS             complement(5121..>5566)
                     /locus_tag="VD791_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07736.1"
                     /translation="YGWELEGGDAAALYSLGVKSRLTFAAGTGDFTLGNTLNHAAYVR
                     DGSSFPLTLFVTGLNLEFPSNATLWGRPVDFGIHLIHYAYLRQLPFPLLEDVNNEVRE
                     EAEIAFSLKTRTPVSIAGFDADMVGLAFRVGPDVEGIRLFFTLPY"
BASE COUNT          840 a         1946 c         1873 g          907 t
ORIGIN      
        1 cggccgatcc aggcgcgcgg cttcaacaac taccagcaca cgctgttcaa ggagttcttc
       61 tgctacggcg gtgtggcttc cggcggtccg ctcggcagcg ccgagaccat cgtgcgcaac
      121 ctcaagcacc ccgccccgca agacaacacc gtcccgcgca agttcaacca gttccagcag
      181 cgctagcccg gcgcttggtg cgcgcgtatg agcaatgccg cgcccgctga cgcggtgccg
      241 tcactggcaa tcgccgacgc gccgggcgcc gaaggtctgc agaatcggca ccggggtgcc
      301 acccatgccg atcgtcgcgt tgagcgtccc gtcgaagccg tcgccggtga tggtgacgtc
      361 gcccgtgccg gtgagctcga tctgcccggt gcaggtcatg ttccagcgcg cgcggttgcc
      421 ttcctgacgc aggtccgaca cctggcagcc gccgccagcg ccggtgagct tggccaccag
      481 atcctgcgcg gattgctggt tgatgcactg gcgcatcacc aggggttcgg cggtggccgg
      541 ttgtccgccg atctccatgc gcacggtgac ttcccacagg ccttcgcgca atgcgtcggc
      601 agacgccgac aggggcatca gcagggcggt gcaaagcagc agcgggcgta tgacgggcgt
      661 gcgcatgaga acctccggtg gacgatggcc tgcgccgacg caagggtcgt gccccaacat
      721 ggcgctgcta gagcagcaca agagtgtgac tccggccggc gcgcaagcgc gccttaactt
      781 gcccgcagcc tggtgctgct agagcagcac caggttgtcc ctgtggatca actcgggggc
      841 gtcgatgtag ccgagccgcg cttcgatctc gtggctgggc acgcgcagga tgcgccgggc
      901 gtcctgggca ctgtagttga ccaggccgcg cgcgacctcg tgcccgtccg gatccaggca
      961 gctcaccacc tcgccgcgct cgaagtcgcc ggcgagatcc tgcacgccga tcggcagcag
     1021 actcttgcca tttcgcgtga gcgctcggac cgcgccggcg tccagcatca gcttgccgcg
     1081 cacctgcagg tggtcggcca gccactgctt gcgggccgcc agtgtcatgg tctcggcgac
     1141 gagctgggta ccgatcatct cgccatccac caaccgcagc agcacccgtg gctcgcgccc
     1201 cgaggcgatg atggtgtgcg caccgctgcg cgccgcgcgc ttggcggcgc ccaccttggt
     1261 gagcatgccg ccgctgccga cgtggctgcc gaccccgccg gccatctgct ccagcgcggg
     1321 atcgccggct ttggcttcgg tcaccagggt ggcgtcgggg tggcgccgcg gatcgcgggt
     1381 gtagaggccg gcctggtcgg tgaggatcac cagcgcctcg gcttccacca ggttggtcac
     1441 cagcgcaccc agggtgtcgt tgtcgccgaa cttgatctcg tcgttcgcca cggtgtcgtt
     1501 ctcgttgatc accgggatca cgtcgagctg gagcagcgtg tgcagggtgg agcgggcgtt
     1561 gaggtagcgc ttgcggtcgg acaggtcctc gtgggtgagc aggatctgcg cggcgtgcag
     1621 gccgtggttg cggaagcagc tctcgtaggc ctgtaccagt cccatctggc ccaccgcggc
     1681 ggcggcctgc agctcgcgca gcgcatgcgg ccgcttgtgc cagcccagcc gctgcatgcc
     1741 ctcggccacg gcgccgctcg acaccagcag cacctggcgg ccgcggcgct tgagctcggc
     1801 gatctgttcg gcccactgcg ccagggcttc gtggtccagg ccctggccct ggccggtgac
     1861 caagctgctg cccaccttga ccaccagccg cttggcgttt gccagcacgg acggcatgcg
     1921 cgctactcct cgcggtcggc ttgcccaggg ttgatcagcg tctcggcgtg gagcaccgtt
     1981 tcggcctcgc cagccgcgcc ggtgccgggc gaggactccc cgccggcggc ctgcgccccg
     2041 gcggcgtcct gcgccaggaa atccgcgatc gcgtgcgtca gctccgcaca gccctcgccg
     2101 gacaacgccg atatggtaaa cacgcgcgcc gcttcgggcg actccgtccc ggccagggcc
     2161 cggtagcgat cgaggaacgc gcgcatccgc atgccgcgtt ccgcggcggg cagcaggtcg
     2221 atcttgttga gcaccagcca gcgcggcttg cggtacaggt cctcgccgta tttgcgcagc
     2281 tcaagcgcga tggcatgcgc atcggccgcc ggaccggcgc cttcctcgcc gggtttctct
     2341 ccaatggccg gcgtggcgat gtccaccagg tgcagcagca ggcgcgtgcg cgcgaggtgg
     2401 cgcaggaact ggtggccgag cccggccccc tccgccgcgc cttcgatcag ccccggaatg
     2461 tcggcgacga cgaaggcgcg attgacgtcc acgcgcacca cgccgagatg cgggtgcaaa
     2521 gtggtgaacg gatagtcggc cacccgcgga cgcgccgccg aaaccgcccg gatgaaggtg
     2581 gactttccgg cgttgggtag ccccagcaga ccgacgtcgg ccagcacttt gagctccagg
     2641 cgcaaccggt gcgattcgcc cggctcgccg cgcgtgaact ggcgcggcgc gcggttggtg
     2701 ctggacttga agtggaggtt gcccagaccg cccttgccgc cgcgagccag cacgacgcgc
     2761 tgcccatggg ccgcgagatc gaccagcacc tccccggtgt ccgcgtcgct caccaccgtc
     2821 ccgaccggga aggtgagcgt gaggtccttg ccggcggcgc cgtagcgatc accaccctgg
     2881 ccgctgtcgc cgttgcgggc gcggtggatg cgtgcgaaac ggtattcgac cagcgtgttg
     2941 acgttgcggt cggcgaccgc gtacacgctg ccgccgcgcc cgccgtcccc gccgctgggc
     3001 ccgccccggg ggatgtactt ctcgcgccgg aacgcggcca caccgtcccc gcccttgccg
     3061 gcgtggactt cgatgacggc ttcgtcgatg aacttcatcg ctggaaacga aaaagcccta
     3121 ccgcaacgat agggcttttc ggcgcaaccg gctggagcgc taggcgctgg ggggctcgac
     3181 catcaccgtc tgcttgccga gcggcccctt gaccgcgaag cgcacgatgc cgtcgatctt
     3241 ggcgaacagc gtgtggtccc tgcccacgcc cacattcgcg cccgggtgga actgcgtgcc
     3301 gcgctggcgc acgatgatgc tgccggccgg cacgcgctgg ccgccgaagc gcttgacgcc
     3361 caggcgcttg gcgttcgagt cgcgcccgtt gcgcgagctg ccgcctgctt tcttgtgtgc
     3421 catgccttgc tctccttacc gcagcgagat gccgctgatc tggatttccg tgtagttctg
     3481 ccgatgccct tgcgtcttgc gatagtgctt gcgccggcgc atcttgaaga tcttcacctt
     3541 gtcgccgcgg ccgtggccca gcacggtggc cttgacgctg gccccggaca gggtcggggt
     3601 gcccatcgcg acgttgtcgc cgtcggccac catcagcacc tggtcgagcg ttatctcggc
     3661 gccgacgtcc gcgggcagcg actcgacctt gagcttttcc ccggcgctca cgcggtactg
     3721 cttgccgccg gtcttgatca ccgcatacat ggttcaaggc cctgagaagc ttctcgaaaa
     3781 acggcgcagt atacctgccg ccccccaagt gagtcaaacc gaaccgggtc aaaccggcct
     3841 cgctcgagct gaaacaccgc tgcactgtgc aacacccgga ggcccggcgc cggcgggccc
     3901 gcccgggacg gcaatggtat ccttgcaccc gccgccccgc ctctccggat ccccgcaatg
     3961 accattgcca cgctcgagcc agtccaccgt ctcatcgccg aagagatgcg ggcggtggac
     4021 gaggtgatcc gcgtgcggct gcactccgac gtggtgctgg tgcgccaggt cgccgagcac
     4081 atcgtcggca gcggcggcaa gcgcctgcgg cccgcgctgg tgatcctggc cgcgggggcg
     4141 tgcggctaca gcggcagcgc gcactacacc ctggcggcgg tggtcgagtt catccacacc
     4201 gccacgctgc tgcacgacga cgtggtggac gcctcccagc tgcgccgcgg caaggccacc
     4261 gccaactccc tgttcggcaa tccggccagc gtgctggtgg gcgacttttt gtactcgcgc
     4321 gcgttccaga tgatggtgga cctgaaggac atgcgcgtga tggaggtgct ggccaacgcc
     4381 accaacgtca tcgccgaggg cgaggtgctg cagctgctca actgccacga tgcggaggtg
     4441 accgagcacg attacctgca cgtgatccgc tgcaagaccg ccaagctgtt cgaagcggcc
     4501 gggcagctgg gcgcgatcgt cagcaacgcg agcccggagg tggagcaggg gctggcccgc
     4561 tacggcatgc atgtgggcac cgccttccag ctgatcgacg acgcgctcga ctactccggc
     4621 gatcacgcgc agaccggtaa gaacctgggc gacgacctgg ccgagggcaa gccgacgctg
     4681 ccgctgatcc acgccatgca gcgcggcagc gcggagcagt cgcgcatcat ccgcgccgcc
     4741 atcgagcacg gcgggctcga cgagttcgct ccggtgctcg ccgccgtccg cgagaccggc
     4801 gcgctcgagt acacgcgccg gcaggcggac gccgaggcga agcgcgcggt ggccgcgctc
     4861 gaggcgctgc cgcgttcaag ctaccgggag tctttgctac aattggcgtc cttcgccgta
     4921 tcccgcagct actgagcgct cggtagccgt ccgcaaggca catcggggtg tagctcagcc
     4981 tggtagagta ctgcgttcgg gacgcaggag tcggaggttc aaatcctctc accccgacca
     5041 ctgaattgac gtgatacgtg attcgtgaac accggcacgc tttcacgaat cacgaatcac
     5101 gattcacgaa tcacggctgt tcagtacggc agggtgaaga acagccggat cccttccacg
     5161 tccggaccca cgcggaaagc gagccccacc atgtccgcgt cgaagccggc gatcgaaacc
     5221 ggtgtgcgtg tcttcaggct gaaggcgatc tcggcctcct cgcgcacctc gttgttgacg
     5281 tcctccagca gcggaaaagg cagctgtcgc aggtaggcgt aatgaatgag atggatgccg
     5341 aagtccaccg gccgccccca cagcgttgcg ttgctgggga actccaggtt cagtccggtc
     5401 acgaacagcg tcagcgggaa cgatgaaccg tctctgacgt acgccgcgtg gttgagcgtg
     5461 ttgcccagcg tgaagtcgcc ggtgccggcg gcgaacgtga gacggctctt cacgccgagg
     5521 ctgtagaggg cggccgcgtc cccgccttcg agctcccagc cgtagc
//
LOCUS       DASPGE010000216         4237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1229397, whole genome shotgun sequence.
ACCESSION   DASPGE010000216 DASPGE010000000
VERSION     DASPGE010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229397"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VD791_06895"
     CDS             complement(<1..796)
                     /locus_tag="VD791_06895"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HXV07737.1"
                     /translation="MPSLLELQTASVRGVFDPAEAAIAELIVPAGIRAEARLAVYRRN
                     VLGNYAAALRDVYPVVLRLVGEPFFDRLARDYATQTPSRSGDLHDFGGELGAFLETLP
                     SVRELPYLADVAALEWAVHRSFHARHAPALDPARLAAVLPERLPDLRFELHPAARLLR
                     SPYPVLTIWRVNQPQWSGDQAVDLALGAEQVLIVRRALEVALEPLSAAEYAMLEALDA
                     GGNLGGALAAALAVDAEFDLQAFLAAALTVLRGYCAAGRPDVKVTPW"
     gene            complement(780..1637)
                     /locus_tag="VD791_06900"
     CDS             complement(780..1637)
                     /locus_tag="VD791_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HXV07738.1"
                     /translation="MTAAAPSPLRDTVPSTAGIGLRAPHYRELLDTRPAVGWLEVHSE
                     NFFGEGGQPHWFLERLRAHYPLSLHGVGLGLGSVDPLDGVHLDKLERMIARYQPGLVS
                     EHLCWSAAGGRHVNDLLPLPYTEEALAHVCARVHRVQERLRRQILVENVSSYLRFRHS
                     TIPEWEFLAEVIRRTGCGLLLDVNNVYVSAVNHGFDAHEYLAAIPAAAVQEIHLAGFE
                     TTPDLLIDTHGRAVADPVWALYRETLARIGPRPTLIEWDTDIPPLSVLLAEARKADVL
                     LGHTHAVPA"
     gene            complement(1643..1903)
                     /locus_tag="VD791_06905"
     CDS             complement(1643..1903)
                     /locus_tag="VD791_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HXV07739.1"
                     /translation="MKQTDLIIRTAVASVLAMGLAGAGQALAAKEDMEKCAGVAKAGK
                     NDCGTSKHSCSGQATADRSPDEWIYVPKGTCDKIAGGKVVSK"
     gene            2163..2558
                     /locus_tag="VD791_06910"
     CDS             2163..2558
                     /locus_tag="VD791_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07740.1"
                     /translation="MSQKAVEIILMRQLASYLAMPILLFDPAGTLLFYNEPWEAISGM
                     RFDETGELKLEEWSSLFHLTAEDGSPLPLEARPSVTALQQRQASHRVFWLMGADGVRK
                     KLEATGFPLEGQGGRHLGAVVILWEAAQA"
     gene            2555..3394
                     /locus_tag="VD791_06915"
     CDS             2555..3394
                     /locus_tag="VD791_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV07741.1"
                     /translation="MRATLWGTRGSLATPGPETVRYGGNTSCVEVKGADGTILVLDAG
                     TGIRRLGGRLGPEVGRVNVLLTHLHMDHIQGLGFFRPLDQPGQEVHIWGPPSTTLDLR
                     ARVSRYLSPPLFPVRLRELPCCLTLHDVPLTPEGIRIGELRICAALVCHPGPTVGYRI
                     SENGATLAYIPDHEPLLGASRLPSSDWISGFDLAARADLLIHDSQYSATQYAKHVGWG
                     HSAIPHVLEFAARTGVKRLVTFHHDPRHDDAALDRLVDEARSSADLPFEILPGVEGAS
                     FEC"
     gene            3465..3538
                     /locus_tag="VD791_06920"
     tRNA            3465..3538
                     /locus_tag="VD791_06920"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3499..3501,aa:Ile,seq:cat)
     gene            3658..>4237
                     /locus_tag="VD791_06925"
     CDS             3658..>4237
                     /locus_tag="VD791_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07742.1"
                     /translation="MRPILVALSLAAVIAASPARAQLAISGGIEYFQWKEDTAPIQVR
                     EDGPMFVLGLDYTQRRDRGLLFAYRGKLYIGDVDYDGAELFAPSDPADGTTSYTGTAH
                     EVQLRYRLAPQRGYWLDMLASAGVDLWERELSDVQQEDYTIAFVRLGVELNATGDRGW
                     TFGAGLKYPVWTEEDAHFDDLGFDQNPELEPEG"
BASE COUNT          656 a         1457 c         1453 g          671 t
ORIGIN      
        1 cccacggtgt caccttcacg tcgggccggc ccgccgcaca gtagccgcga aggacggtga
       61 gtgccgctgc caggaacgcc tgcaagtcga actcggcatc gaccgccagc gccgccgcca
      121 gggcaccgcc gagattgccg cccgcgtcga gcgcctcgag catcgcgtac tcggccgcgg
      181 acagcggctc cagcgccacc tccagcgcgc gccggacgat cagcacctgc tctgcgccca
      241 gggccagatc caccgcctga tccccgctcc attgcggctg attgacgcgc cagatggtca
      301 gcaccggata cggcgagcgc agcagccgcg cggccgggtg cagctcgaac ctcaggtcgg
      361 gcaggcgctc gggcagcacc gcggcgaggc gagccggatc gagtgccggc gcatgccgtg
      421 cgtggaagct gcgatgcacg gcccactcca gagccgcgac gtcggcgagg taggggagct
      481 cgcgcaccga aggcagtgtc tcgaggaacg cgccgagctc gccgccgaaa tcgtgaagat
      541 cgccggagcg cgacggggtc tgcgtcgcgt agtcgcgcgc gagccgatcg aagaacggct
      601 cgccgaccag gcgcagcacc acgggataca cgtcgcgcaa cgccgcagcg tagttgccca
      661 gcacgttgcg ccggtagacc gccagccgag cctccgcgcg tatgccggcg ggcacgatca
      721 gctccgcgat cgcggcctcg gccggatcga acacgccgcg cacggacgcg gtctgcagct
      781 caagcaggga cggcatgcgt gtgcccgagc agcacatcgg ctttgcgggc ctcggcgagc
      841 agcaccgaga gcggtggtat gtcggtgtcc cactcgatca gggtcgggcg cgggccgatg
      901 cgtgccagcg tctcgcggta cagcgcccat acggggtcgg cgaccgcacg cccgtgcgtg
      961 tcgatcagca ggtccggcgt ggtctcgaag ccggccaggt gaatctcctg caccgcggcg
     1021 gcgggaatcg cggcaaggta ctcgtgcgcg tcgaaaccgt ggttgaccgc actcacgtac
     1081 acattgttga cgtccagcag caggccacag ccagtgcggc ggatcacctc ggccaggaat
     1141 tcccactccg ggatggtcga gtgccggaag cgcaggtagc tcgacacgtt ctccaccagg
     1201 atttgccggc gcaggcgttc ctgcacccgg tggacgcgcg cgcacacgtg cgcgagcgcc
     1261 tcttccgtgt acggcaacgg cagcagatcg ttgacatgcc gtccccccgc ggcgctccag
     1321 cacaggtgct cggagaccag gcccggctga tagcgagcga tcatgcgttc gagcttgtcc
     1381 agatgcacgc cgtcgagcgg atcgaccgat cccaggccga gtccgacgcc gtgcaggctc
     1441 aacgggtagt gcgcgcgcaa ccgctcgaga aaccagtgcg gctggccgcc ctcgccgaag
     1501 aaattctcgc tgtgcacctc cagccagccg acggccggcc gggtgtcgag cagttcgcga
     1561 taatgcggcg cgcgcagccc gatccctgcc gtggacggca cggtatcgcg gagcgggctg
     1621 ggcgcggccg cggtcatgcg cgttacttgc tgaccacctt gccgccggcg atcttgtcgc
     1681 acgtgccctt cggcacgtag atccactcgt ccgggctgcg gtcggctgtg gcctgacccg
     1741 agcacgaatg cttgctggtg ccgcagtcgt tcttgccggc tttggccacg ccggcgcact
     1801 tttccatgtc ctccttggcc gccagggcct ggccggcgcc ggcgaggccc atggcgagga
     1861 cgctcgccac ggcggtgcgg atgatgagat cggtctgctt catgatgcgg gactcctcca
     1921 aagcgttgat tgcgggactg tggcgcggcg ctcgcgcgcg gccacctgcc agatacgcgc
     1981 acgatgcccg ttccgattca agttcgggcc ggcagtgcgt tggcggcagc cacgataccc
     2041 caaacccgcg atttcggaat acgccaaccc caatttcgga atcccgcggg ctggcatatg
     2101 atgaccggcg tgccctgcgg ccggccgggg tcgaaggcgt ttttgcggga ggagcgccga
     2161 aaatgtccca gaaagccgtc gagatcatcc tgatgcgcca gctcgcgagc tatctcgcga
     2221 tgccgattct cctgttcgat ccggccggca cgcttctgtt ctacaacgag ccgtgggaag
     2281 cgatctccgg catgcgcttc gacgaaaccg gcgagctcaa gctcgaggag tggtcctcgc
     2341 tgttccatct gaccgccgag gatgggtcgc cgctgccgct cgaggcgcgc ccttcggtca
     2401 ccgccctgca gcaacgccag gcctcgcatc gcgtgttctg gctgatgggc gccgatggcg
     2461 tgcgcaagaa gctggaggcg accggcttcc cgctcgaggg ccagggcgga cgccacctgg
     2521 gcgcggtcgt gatcctgtgg gaggcggcgc aggcatgaga gccacgctct ggggaacgcg
     2581 cggctcgctg gccactcccg ggccggagac cgtgcgttat ggcggcaaca cctcctgcgt
     2641 cgaagtgaaa ggcgcggacg gcacgatcct ggtgctggac gcggggaccg gcatccggcg
     2701 cttgggcgga aggctcgggc cggaagtggg tcgcgtgaac gtgctgctga cgcacctgca
     2761 catggaccac atccaggggc tgggcttctt ccggcccctc gatcagcccg ggcaggaggt
     2821 gcacatctgg ggtccgccgt ccaccactct ggatctgcgc gcgcgcgtca gccgctacct
     2881 gtcgccgccg ctgttcccgg ttcggctgcg ggagctgcct tgctgcctga cgctgcacga
     2941 cgtgccgctt acgcccgagg gtattcggat cggcgagttg cgcatctgcg cagcgctcgt
     3001 gtgccacccc ggcccgaccg tcggatacag gatcagcgaa aacggcgcca cgctcgccta
     3061 catccccgac cacgagccgt tgctcggagc gtcccgcctg ccgtcctcgg actggatctc
     3121 cggtttcgac ctcgctgcgc gcgccgacct gctgatccat gattcgcaat acagcgccac
     3181 gcagtatgcg aagcacgtcg gctggggcca cagcgcgatc ccgcatgtgc tcgagttcgc
     3241 ggcgcgcacc ggcgtgaagc gcctggtcac cttccaccac gatccgcgcc acgacgacgc
     3301 ggcgctggat cgcctggtgg acgaggcgcg cagctcggcc gacctgccgt tcgagattct
     3361 tcctggcgtc gaaggcgcat ccttcgagtg ctagcgcgca cggcgccgag gcggcgcatg
     3421 cttaacaccc cttggccggt gtgctttaat ggcgcgcttg cgcggggccc gtagctcagt
     3481 tggtcagagc agaggactca taatcctttg gtcctgggtt cgagtcccag cgggcccact
     3541 ctggcgtgcc gccggcgatg ccggaggctt ttcgtcccgg ctgctgcgga ccactggcat
     3601 atgagttgcg tcggctggcc gccggcgctt tcctccacag cccgcaagga gccgctcttg
     3661 agaccgatcc tcgtcgccct gtcgctggcc gccgtcatcg cggcgtcgcc ggcgcgcgcg
     3721 cagctcgcca tcagcggcgg tatcgagtac ttccagtgga aggaagacac cgcaccgatc
     3781 caggtgcggg aggacgggcc gatgttcgtg ctgggcctcg attacacgca acgcagggac
     3841 cggggcctgc tgttcgccta tcgcggcaag ctctacatcg gtgacgtgga ctatgacggt
     3901 gcggagctgt tcgcgcccag cgaccctgcc gacggcacca cttcgtatac cggcactgcc
     3961 cacgaggtgc agctgcgcta ccgtctggct ccgcagcgcg gttactggct ggacatgctt
     4021 gccagcgcgg gcgtggacct atgggagcgc gagctgagcg acgttcagca ggaggactac
     4081 acgatcgcct tcgtgcgcct gggggttgag ctcaatgcca cgggcgaccg cggctggacg
     4141 ttcggcgccg ggctgaagta cccggtctgg accgaagagg atgcgcactt cgacgacctg
     4201 ggcttcgacc agaacccgga gctcgagccg gaaggaa
//
LOCUS       DASPGE010000217        19859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1346190, whole genome shotgun sequence.
ACCESSION   DASPGE010000217 DASPGE010000000
VERSION     DASPGE010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19859
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346190"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VD791_06930"
     CDS             complement(<1..854)
                     /locus_tag="VD791_06930"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HXV07743.1"
                     /translation="MPRIDAALVWFRRDLRSRDHAALYHALAQARRVYCAFVFDTEIL
                     DALDTRADRRVEFIWESVAELKRALEHAGGGLIVRRARARDELPALAARLGVPAVYAN
                     HDYEPQAIARDLAVARALAASGSELRTFKDQVIFERDEVLTRAGTPFSVFTPYRNAWL
                     ARLQDTDLRPFPVERHRTALARPPESTLPSLEALGFARTNLREMRMPTGMSGARALFE
                     DFRGRMSRYRERRDYPALRGPSYLSVHLRFGTISVRELVRAARGVGDAGATAWLSELI
                     WRDFYFMIL"
     gene            984..1871
                     /locus_tag="VD791_06935"
     CDS             984..1871
                     /locus_tag="VD791_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXV07744.1"
                     /translation="MTLNISAVERDTGLSKDTLRVWERRYGFPEPARDANGERVYPLS
                     QVRKLQLIKRLMDRGHRPGKIVSLGLEELTGLSATAPRRAPGEQIEILARLIATHQLP
                     ELRRQLAQALMKQGLQRFVIDTIAPLNRAVGEAWMRGDIGVFEEHLYTEQIQGVLRNA
                     LAALQPQGRGPRVLLTTFPHEPHGLGLLMVEALLAVEGACCVPLGTEMPLAEIARAVR
                     ANRIDVLALSFSAAFNEKQGAAGLAELRAMLPGAVAIWAGGECVARMRKPVEGVELVG
                     ELATVVEMVKQWRARHAGA"
     gene            1934..2416
                     /locus_tag="VD791_06940"
     CDS             1934..2416
                     /locus_tag="VD791_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HXV07745.1"
                     /translation="MKAADFEAKICPPERLAPRLEGLVRPLVFTNGVFDILHRGHVTY
                     LAQARSLGASLLLALNSDASARRLGKGENRPFNTMEDRAAVVAALESVDLVTWFEEDT
                     PYELILACRPQVLVKGGDWPIDQIVGSSEVQSWGGKVHAIPFLFDRSTTRLVAGIRGR
                     "
     gene            2471..4222
                     /locus_tag="VD791_06945"
     CDS             2471..4222
                     /locus_tag="VD791_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HXV07746.1"
                     /translation="MFIKTRPGFWRDLWSLIHPYWRSEEKVSAWALLLSVVGLTLAMV
                     YMEVLFNHWNNAFFNSLQNKDKAEFFRQMGRFCVLAAIFIVIYIYSVYLNQWLQIRWR
                     RWLTDRYLKEWLSERTYYRMQLTGNGADNPDQRIAEDLKLFVDSTLDLSLGFLNAVVT
                     LFAFLTILWGLSGALEFTVSGQTYVIYGYMVWVAFAYAVIGTWLTHKVGRPLIGLNYD
                     QQRYEADFRYNLVRFRENTEGVALYRGEQGELSGFRERFAHVARNWWSIMKRIKLLNS
                     FRYGYNQAAIVFPYLVAAPRYFSGSFTLGDLTQTASAFGQVQGSLSWFINAYVSFANW
                     KATVDRLTGFHNAVMGAQAQQRAHPGVAVASGESDNLELQHVDLLLPSGRVLVSDANL
                     TIREGMHVLVRGSSGCGKTTLFRAIAGIWPFGHGAVKLPKNFQGLFLPQRPYFPLGTL
                     RAAVAYPAAPDAFADEQIYDALVGVGLPRLAERLNEEANWAMQLSGGEQQRVALARAL
                     LHRPKWLFLDEATSALDEAAQADLHRLLHERLEETTIVSIAHRPGVGEFHDTEVELAH
                     EEDSEARHVRMTQSATV"
     gene            complement(4283..4726)
                     /locus_tag="VD791_06950"
     CDS             complement(4283..4726)
                     /locus_tag="VD791_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07747.1"
                     /translation="MRRLWHPRLVSFLLGLALLALGTHWALEFGSRRLAAEPAVATPA
                     GDAAPRTQALDTEPLSRLFGGSSAPRTGNVALVGIVASGALDRDVALVSVDGAPAVMA
                     GVGDTLPDGALVVEVRGYGIVVRRGDVLQEILLPVPPSPGIERAP"
     gene            4848..5282
                     /gene="gspG"
                     /locus_tag="VD791_06955"
     CDS             4848..5282
                     /gene="gspG"
                     /locus_tag="VD791_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HXV07748.1"
                     /translation="MRNGVRATGVGGLTLIEILVVVAILGVLAALIVPRVVGRTDEAR
                     AVAAKQDIATILQALRMYRLDNDRYPTSAQGLDALVLRPGSEPIPRNWKQYLDKVPKD
                     PWGQPYQYLSPGIHGEVDVFSFGADGTTGGGGIDAEIGSWQL"
     gene            5254..5763
                     /locus_tag="VD791_06960"
     CDS             5254..5763
                     /locus_tag="VD791_06960"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HXV07749.1"
                     /translation="MPRSARGSCERGSPRGDVTRVRLARALRGFTLIELLVAITILAI
                     AAAIAALSVASGEGRRVDEETRRLAALFRLAQDEAAMSGTMLVWEADVHGYRFRRLDR
                     GDGEGEPSPQGDLLRARRWPFGVSRVEAQPIVFGREPLLQPGVIRLTTASRDIRLALD
                     PFGNLTEAP"
     gene            5760..6134
                     /gene="gspI"
                     /locus_tag="VD791_06965"
     CDS             5760..6134
                     /gene="gspI"
                     /locus_tag="VD791_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006580259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HXV07750.1"
                     /translation="MTRSRCAGFTLLEVLVGLAIAAVALMASIRAVGSMAQTNAALEL
                     RALAQLSAHNRVAELRATGAFPAVGTRTAPCPQGRTQLQCAEETQPTPNPLFRRVEVR
                     VAQPSAPDAVLARLTAVLPRVP"
     gene            6131..6745
                     /locus_tag="VD791_06970"
     CDS             6131..6745
                     /locus_tag="VD791_06970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXV07751.1"
                     /translation="MRPPHRTRAPGFSLLELLVAIALLGVLAVLLWRGLGGVIAQRER
                     IDRDAAHVERVIRLLAQLERDLAQRAPDVVIPAGVEASGGLPRALEVRLQREGNAQLV
                     ILRTAPDAGGPLRLERVRYFVAQGALLREASPPSSTWPLAPPPPPLRLLDAVERLSVR
                     LLRLEPRSGAGPSAAGAGSDARVTGIEVSVERANGERYVRVVAL"
     gene            6742..7668
                     /gene="gspK"
                     /locus_tag="VD791_06975"
     CDS             6742..7668
                     /gene="gspK"
                     /locus_tag="VD791_06975"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HXV07752.1"
                     /translation="MNHRSQRGGAVVMAMLAVAIAATLVAGTFWRQEVWLQRVENELS
                     FAQAQWLMRGVIDWARVILAEDARTSRVDHLGEPWALQIADTRLDDSSGTPAYLTGGM
                     QDAQAKYNLRNLVGPDGIVPAELEVLRRLLAFAEVPETLANPIAQRVLDGVPAPAGEG
                     GPASTGESLGARRQALPLRTLDDLLSVPQIGVAQVQALRPYLTVLPRPTPVNANTASA
                     QVLAGTIADLSLPDARRLLASRDRAHFKDLHDVVSRMSGRPLQADPTRVGIDTQFFLM
                     QGHIVHRRARLHAQALLLRAGNSVQLVWVREL"
     gene            7668..8846
                     /gene="gspL"
                     /locus_tag="VD791_06980"
     CDS             7668..8846
                     /gene="gspL"
                     /locus_tag="VD791_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HXV07753.1"
                     /translation="MTTLRVFLCPLAELGAQSVLAYELLDRRRRIMRRGQAVPALLPK
                     TGRCELVLAAPDVLLVDAALPKMRGARLRAALPMLVEEFLLGDMEDALVVAGAPDRAS
                     GRATVAMLDRALLQRALGLFAQLRIRVVSATPEPLTLAAAPGCWRVRLRESYGYARTG
                     ERLGIALSPALDAGPPRELALALAQQPVPSAIEIEGECDAAAWEEALRVPVRRVRDEQ
                     TVAPAVALELLQYEAAPDAADWSRWRGPVVLAAALLLLWIAGLNVQAWLLQREERALR
                     AEMANLVRQAFPEVPVVLDPVVQMRQGVGELRAAAGIDSPTDFLPLATRFAQALADDR
                     AVRSIEYRDAVLHVRFADAHLLESAPARLALSSRLAQDGLEVQFSNEGAVVAPRRVRS
                     "
     gene            8860..9351
                     /gene="gspM"
                     /locus_tag="VD791_06985"
     CDS             8860..9351
                     /gene="gspM"
                     /locus_tag="VD791_06985"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HXV07754.1"
                     /translation="MIAALRSYWLGLSRRERTLSGAGIGIVLCAAAFLVAVEPAWKTQ
                     ARLRTELPELREQAAELAALAQEAERLREHLEARPGTAELKDAIGQSLSRAGLEAASV
                     AVVGEGQWTVRAQAVPAGQWLGWLEQVARELRLRVGSARIARGDAVGLIDVEVTLNAF
                     PKR"
     gene            9332..10093
                     /gene="gspN"
                     /locus_tag="VD791_06990"
     CDS             9332..10093
                     /gene="gspN"
                     /locus_tag="VD791_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HXV07755.1"
                     /translation="MRFPSAERSSWRLRALQLLAALVVVAAIVIARPPAAWLADLLHA
                     HSRLRLIDPQGTLWQGSAQLAVSDGRRALLVPGRLTWRASLAGFLGAKVEIRHPATLA
                     PVRVSIEPGKVEIEAGHAQTPAAVLSALGTPFNTVRPGGTLHVQWSALRLQQEAAHGD
                     VRLEWRDAQSALSPVAPLGDFLVVVKLRGEAGEVALRTLRGPLHMEGRGSLTRGQLAF
                     TGQAFAEEPMRAALLGLIGVLGKRSGDRAQLRLGT"
     gene            10126..12291
                     /gene="gspD"
                     /locus_tag="VD791_06995"
     CDS             10126..12291
                     /gene="gspD"
                     /locus_tag="VD791_06995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HXV07756.1"
                     /translation="MTTKRTRRDGLLALATHAVLALSLAVPLLTGGAQPAKEEKVRLR
                     FVNADIESVVRAVAHFTGKLIVVDPRLKGTLTLTSDKPMTSAQALAALRSALRVQGIA
                     VVESDGVWRVVPEADAKVQPGPVLSRPTRPSAGDHIITQVFQLKYESAPNLLPVLRPL
                     VTASNPISAYAGNNTIVVTDYAGNVRRVAEIIASVDTPSAGATEVIRLQHAIASDIAV
                     LLGRLLDAGGAQGGDPSQRVSVQAEPSSNSLVLRAPSPARAEQVKGLVARLDQPLSQA
                     TGNVHVVPLRNAEAAALARTLAGLAPADSGAAPDYSSSFSGAQQQRAPGMAMLASTQT
                     PARPAEGPMPVAMGGLQGQAMVLADTATNSLIITAPQQIYRQLRAVIDKLDLRRAQVF
                     VECLIAEVTSDQAAEFGIQWQVGLDDIAGSSTNVVGGTNFGGVTQNITSAAQNLSLLG
                     PGLNVGVVKGTITIPGVGEITNLTALARALETVTKANILSTPTLLTLDNEEAQILVGQ
                     NVPLVTGQYVTPGTGGPVTVNPFQTIERQDVGLTLRVRPQISEGGTIKLVIYQEVSSL
                     QRRTVDTADIVLNKRTVETKALVDDGSIVVLAGLVEDNVTSTREKVPLLGDLPLIGAL
                     FRYDTKQQVKTNLMVFIRPVVVRDEAASRLLAADRYDYMRNLQQVTPLPERGPLPDVR
                     GPVLPQLDGSGRPAPAPSPPRTSSPRPGPAAYTPPPGRR"
     gene            12298..13722
                     /gene="gspE"
                     /locus_tag="VD791_07000"
     CDS             12298..13722
                     /gene="gspE"
                     /locus_tag="VD791_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HXV07757.1"
                     /translation="MATLIPYAFARAHSLLPAGEREGRLQLWVTEATPPVAVMEVRRA
                     LARDVEPVMVSRERFEDGLRAAYAQAEGEAAMVVGEVQSEIDLARLMQDLPQVEDLLE
                     ARDDAPIVRMLNALLTQAVQERASDIHIEPFESSSVVRFRVDGTLRDVVRPHRALHAA
                     LVSRIKIMADLDIAEKRLPQDGRILLRLGGRQVDVRVSTLPAGHGERAVLRLLDKEQG
                     RLEPERLGMSPAVLASLEQVVRQPHGIVLVTGPTGSGKTTTLYAMLSALDAATTNIMT
                     VEDPIEYDLPGIAQTQVNPRIGMTFARALRAILRQDPDVIMIGEIRDLETAQIAVQAS
                     LTGHLVLATLHTNDSIAAVTRLLDMGVEPFLLSSSLLAILAQRLVRRLCVSCRTQDSK
                     GQWHPLGCPSCQGSGYRGRAGVYELLLVDDAIRDAIHSGEGEQALRRLAQQRGFRPLR
                     EDGERWVASGETSLEELLRVAREQ"
     gene            13779..14987
                     /gene="gspF"
                     /locus_tag="VD791_07005"
     CDS             13779..14987
                     /gene="gspF"
                     /locus_tag="VD791_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HXV07758.1"
                     /translation="MPSYRFEASDAAGRVEKGVLEADGARAARTQLRARGLAPFALEP
                     LAGGAGETPGWVRLRLRELELTTATRHIAALLSAKLPLATALTAVIEQSERPATREAL
                     SAVRSEVVAGARFADALARFPRLFPQVYRATVAAGEQAGDLALVMERLADYLEQRQAL
                     RGKILSAILYPAVVTLVAVAIVLFLMTYVLPQVVQVFEQTSQSLPLPTRMLLATSAFL
                     RNFGLWLVLLLPALGFALAAWLRRPGPRAAWHALLLRLPLLGSVLRTVDAARFAGTLA
                     MLAASGVPVLRALEGARATLRNSVLAQAVSGAIAQVREGEALARALGATRAFPPMLIH
                     LIASGEATGELARMLDRAAAIQAREVERRLLVLTTLLEPLLILAMGAMVLGIVLAVLL
                     PIIDINQLVR"
     gene            complement(14992..15621)
                     /locus_tag="VD791_07010"
     CDS             complement(14992..15621)
                     /locus_tag="VD791_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07759.1"
                     /translation="MRLVFTVLVLANLALAAYALLFSGQRSRDAALLEQQLNADKVRI
                     IPPPPPAPRRSACLEWGTFSDVELAAARRELERLGLAQRAAESRVPVVAGWWVFIPPQ
                     VSRVEVDRRIAELTALGVTDYFAVEGESAMRNAISLGIFRSEEAAGEFLRGLQARGVR
                     LARMGSREHRVTQTVFVLREPSAQVSAQLAALASRFPGSELRALSDCAE"
     gene            complement(15618..16340)
                     /locus_tag="VD791_07015"
     CDS             complement(15618..16340)
                     /locus_tag="VD791_07015"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HXV07760.1"
                     /translation="MILAIDAGNTRIKWGLWQERGFVAQGSVLTAHAADLADALHMLP
                     RPQQAVGSSVAGDHARAQIEQALAPWGIAVRWISSQASECGVVSGYAAPGQLGSDRWA
                     ALVGAHARRDGACVVVNVGTAVTIDALTADGRFLGGLILPGPDLMAQALAQGTAGLPQ
                     QPGRFQPFPTGTADAIHSGALQATCGAIERMQQAMIDAGHGDPRTLLSGGGAHLVAPL
                     LRHPAVLAPSLVLEGLIEIART"
     gene            complement(16337..17344)
                     /locus_tag="VD791_07020"
     CDS             complement(16337..17344)
                     /locus_tag="VD791_07020"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HXV07761.1"
                     /translation="MTASPAPLAFRALRLLSDGDFHSGEALARALGVSRTTVWKALQG
                     LEAFGVTLFKVHGRGYRLVTPMDWLDAAQVRRHLGARAETFQVHVVDAVDSTNTLLLA
                     EALAGAPSGLALAAEVQTGGRGRRGHRWHSGLGGGLTFSMLWRFEQGVGNLGGLSLAV
                     SVALVRALRELGGDEVQLKWPNDLLCRYQKLGGVLTELEGDVMGPSAAVIGIGVNVRV
                     DPGVRQRIDQAVTDLTAAGVRQDRNRVLGALLRHVADALELFAAHGFAPLKDEWMRYD
                     ALAGRAVAAIMPDRTEHAGIAAGVADDGALQLQTTAGLRRFHSGELSLRPLEGGLRRA
                     G"
     gene            17539..17982
                     /locus_tag="VD791_07025"
     CDS             17539..17982
                     /locus_tag="VD791_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXV07762.1"
                     /translation="MFKKILLPTDGSDLSKKAVKKGVDFAKSIGAGVVGFFSPEDYRA
                     LMYSEYIPPSLLSQEEFEANAKKAAEKHLAFIEKTAAAAGVPYEGYFLSSIAPWEAIV
                     EAAKKKRCDLIFMASHGRTGLAGLVLGSQTTKVLTHSKIPVLVYR"
     gene            complement(17979..19700)
                     /locus_tag="VD791_07030"
     CDS             complement(17979..19700)
                     /locus_tag="VD791_07030"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HXV07763.1"
                     /translation="MTANWAATVAFLAILALAGLAQDARGEEPAATATRPDVPGATAP
                     VSGFESAEREAHWPTAPVALDGRTLLRVRGSPSFPPEQRAARIEARVEEAAADRAIPA
                     GAIRVVESGDMSMIVAGERRIMAVVEADASIEGVQRQRLAGLHRDAIAEAVVRYRRDR
                     EPRALLMSTVYSVSALAALAAVVLALRWPFRRLAAVLEARYRARVHDLGIQSFRLLRA
                     EQLWQGLRSGLSFLHLLAVLVLAYLCLQFILSQYPATRVFGERLLSLVLGPLGSIGSS
                     LLSALPSLVFLAVLAVVTRYLLRLLHLFFAAVEHGTVVLRDFDREWAMPTYRIVRLLV
                     VAFAVVVAYPYIPGSESAAFKGVSIFLGVMFSLGSTSVIGNIIAGYTMTYRRAFRVGD
                     RIQVEGLIGDVTEIRLQVTHLRSPKNEEIVVPNSILLNGNVINYSTLARERGLILHTT
                     VGIGYETPWRQVEAMLLMAAQRTAGLLAEPPPFVLQKALGDFCITYELNVFCDTPSSM
                     ARLYTELHRNILDVFNEYGVQIMTPAYEGDPERPKVVPKDSWFLAPARVGASLPGEAQ
                     RRSESGG"
BASE COUNT         2969 a         6965 c         7030 g         2895 t
ORIGIN      
        1 aggatcatga aatagaagtc gcgccagatc agctcggaca gccaggcggt ggcgccggcg
       61 tcgcccacgc cgcgcgcggc gcgcaccagc tcgcgtaccg agatcgtgcc gaaccgcaga
      121 tgcaccgaga ggtaggacgg cccccgcagc gcggggtagt cgcggcgctc gcggtaccgc
      181 gacatgcgcc cgcggaaatc ctcgaacagc gcgcgcgccc cggacatccc ggtcggcatg
      241 cgcatttccc gcagattggt gcgcgcgaag ccgagcgctt ccagcgacgg cagggtggat
      301 tcgggcggcc gtgccagcgc ggtgcggtgc cgctccaccg ggaatggacg cagatcggtg
      361 tcctgtagcc gcgccagcca cgcgttgcgg tatggcgtga acacgctgaa cggcgtgccc
      421 gcgcgggtca gcacctcgtc gcgctcgaag atcacctggt ccttgaaggt gcgcagctcc
      481 gagcccgaag cggccagcgc ccgcgccacg gcgaggtcgc gcgcgatggc ctgcggctcg
      541 tagtcgtggt tggcgtacac cgccggcacg cccagtcgcg ccgccagcgc cggaagttca
      601 tcgcgcgcgc gtgcgcggcg cacgatcagg ccgccgcccg cgtgctcgag cgcacgcttc
      661 agctcggcca cgctttccca gatgaactcc acgcggcgat cggcccgggt gtcgagcgcg
      721 tccaggatct ccgtgtcgaa cacgaaggcg cagtacacgc ggcgcgcctg cgcgagggcg
      781 tggtacagcg ccgcgtgatc gcggctgcgc agatcgcggc ggaaccagac cagcgcggcg
      841 tcgatgcgcg gcactatggc tactcctgtc ctagtcacaa cgggcttcag tttcgagaat
      901 atccagtaca ctgcctggac cgtcaattat ttgtccagga cgaaccggcc gagcccggtt
      961 cagcccctgt ggaaagaacc caattgactc tgaatatcag tgcggtagag cgcgacacgg
     1021 ggctgtccaa ggacaccctg cgcgtctggg agcgccgtta cggctttccc gagccggccc
     1081 gcgacgccaa cggcgagcgc gtctatccgc tgagccaggt gcgcaagctc cagctcatca
     1141 agcggctgat ggaccgcggc catcgaccgg gaaagatcgt ttccctcggc ctggaggagc
     1201 tgactgggtt gagcgcaacg gcccccaggc gcgcgccggg tgagcagatc gagatcctcg
     1261 cgcgcctgat cgcaacgcat cagctcccgg agctgcgcag acagctcgcc caggccctga
     1321 tgaagcaggg gctgcaacgc ttcgtgatcg acaccatcgc gccgctgaat cgggccgtgg
     1381 gcgaggcgtg gatgcgcggc gacatcggcg tgttcgagga gcacctgtac accgagcaga
     1441 tccagggcgt gctgcgcaac gcgctcgccg cgctgcagcc acaggggcgt gggccgcgcg
     1501 tgctgctcac cacctttccg cacgagccgc acggcctcgg actgctgatg gtggaagcgc
     1561 tgctcgcggt ggagggcgcc tgctgcgtgc cgctcggcac cgagatgccg ctggcggaga
     1621 tcgcccgggc ggtgcgcgcg aaccgcatcg acgttctggc gctgtccttc agcgccgcgt
     1681 tcaacgagaa acagggcgcc gccgggctcg ccgagctgcg cgcgatgctg ccgggcgcgg
     1741 tggcgatctg ggcggggggc gagtgcgttg cacgcatgcg caagccggtc gagggcgtcg
     1801 agctggtcgg cgagctggcc accgtggtcg agatggtcaa gcagtggcgc gcccggcacg
     1861 ccggcgcctg aagcgagctc tacccgccgc gcccttaccg tgcgcggctc cggcccggta
     1921 tgatgcagag gcgatgaagg ccgccgactt cgaagccaag atctgcccgc ccgagcggct
     1981 ggcgccgcgg ctggaggggc tggtgcggcc actggtgttc accaacggcg tgttcgacat
     2041 cctgcaccgc ggccacgtga cctatttggc gcaagcccgc tcgctggggg ccagcctgct
     2101 gctcgcgctg aacagcgacg cctcggccag gcgcctgggc aagggcgaaa atcgcccgtt
     2161 caacaccatg gaggatcgcg ccgccgtggt ggcggcgctg gagtcggtgg atctggtcac
     2221 ctggttcgag gaggacacgc cttacgagct gatcctcgcc tgccgcccgc aagtgctggt
     2281 caaaggcggc gactggccga tcgaccagat cgtcggctcc tcggaggtgc agtcctgggg
     2341 cggcaaggtg cacgcgatcc ccttcctgtt cgaccgctcg actacccgcc tcgtggccgg
     2401 cattcgcggc cgctagagac cggctcgcgc cgccgcgcaa atacaagaaa acacatccac
     2461 tggatttccc atgttcatca agacccgccc cggcttctgg cgcgacctgt ggtcgctgat
     2521 ccatccctac tggaggtccg aggagaaggt ctcggcctgg gcgctgctgc tgagcgtggt
     2581 ggggctcacg ctcgccatgg tgtacatgga agtgctgttc aaccactgga acaacgcctt
     2641 cttcaactcg ctgcagaaca aggacaaggc cgaattcttc cgccagatgg gccgcttctg
     2701 cgtgctcgcc gcgattttca tcgtgatcta catctacagc gtgtacctca accaatggct
     2761 gcagatccgc tggcgccgct ggctcaccga ccgctacctg aaggagtggc tttccgagcg
     2821 cacttactac cgcatgcagc tcaccggcaa cggcgccgac aaccccgacc agcgcatcgc
     2881 cgaggacctc aagctgttcg tcgattccac gctcgatctg agcctcggct tcctgaacgc
     2941 ggtggtcacg ctgttcgcgt tcctgaccat cctgtggggc ctgtccggcg ccctcgagtt
     3001 caccgtcagc ggccagacct acgtgatcta cggctacatg gtgtgggtgg ccttcgctta
     3061 cgcggtgatc ggcacctggc tcacgcacaa ggtcggcaga ccgctcatcg ggctgaacta
     3121 cgaccagcag cgctacgagg cggacttccg ctacaacctg gtgcgcttcc gcgagaatac
     3181 cgagggcgtg gcgctgtacc gcggcgagca gggcgagctg tccggcttcc gcgagcgctt
     3241 cgcgcacgtg gcgcgcaact ggtggtcgat catgaagcgc atcaagctgc tcaacagctt
     3301 ccgctacggc tacaaccagg ccgccatcgt gtttccttac ctggtggcgg caccgcgcta
     3361 tttctccggc agcttcacgc tgggcgatct cacccagacc gccagcgctt tcgggcaggt
     3421 gcaggggtcg ttgagttggt tcatcaacgc ctacgtgagc ttcgccaact ggaaggcgac
     3481 ggtggaccgt ttgaccggct tccacaacgc cgtcatgggc gcgcaggcgc agcagcgcgc
     3541 ccatcccggc gtggcggtgg cgtccgggga gtcggacaac ctcgagctgc agcacgtgga
     3601 cctgttgctg cccagcggcc gcgtgctggt gagcgacgcc aacctgacga tccgcgaagg
     3661 catgcacgtg ctggtgcgcg gcagctccgg ctgcggcaag accactttgt tccgcgccat
     3721 cgccggcatc tggccgttcg ggcacggcgc ggtgaagctg ccgaagaact tccaggggct
     3781 gttcctgccg cagcgcccgt actttccgct gggcacgctg cgcgcagcgg tggcctaccc
     3841 ggccgcgccc gacgctttcg ccgacgagca gatctacgac gcgctggtgg gcgtcgggct
     3901 gccgcgcctg gccgagcgcc tgaacgagga ggcgaactgg gcgatgcagc tctccggcgg
     3961 cgagcagcag cgcgtcgcgc tggcgcgcgc gctgctgcac cggcccaagt ggctgttcct
     4021 ggacgaagcg acctcggcgc tggacgaggc ggcccaggcc gatctgcacc ggctgctgca
     4081 cgaacgcctg gaagaaacca ccatcgtcag catcgcccat cggccgggcg tgggcgaatt
     4141 ccacgacacc gaggtggagc tggcacacga ggaggatagc gaggcacgcc acgtgcgcat
     4201 gacgcagtcg gcgacagtct gatcgccgac gcgacacagc gagcccgcgc cgctgcccga
     4261 ccaggcgtgc tgcagctagg ggctaggggg cgcgttcgat ccccgggctg ggcggcacgg
     4321 gcagcaggat ctcctgcagc acgtcgccac gccgcacgac gatgccatag ccgcgcactt
     4381 cgacgaccag cgcaccgtcg ggcagggtgt cgccaacacc ggccatgacg gcgggcgcac
     4441 cgtccaccga taccagcgcg acatcccggt ccagcgcacc cgaagcgacg atcccgacca
     4501 gcgccacgtt gccggtacgc ggcgccgatg acccgccgaa cagccgggac agcggctcgg
     4561 tgtccagggc ttgcgtgcgc ggcgcggcgt ctcccgcagg cgtggccacg gccggctcgg
     4621 cggccaggcg tcgagagccg aattcgagcg cccagtgcgt acccagggcg agcagggcca
     4681 gtcccaggag gaacgacacg agccgtggat gccacaatct gcgcatgcac ccattatccc
     4741 gcaagcccgg tcctggacgg gcgtcgacgg cagaaaggtt ttccagagcc ggcatcctgt
     4801 gctagcgttg ctggcagggc ggaaatgcgg cggggatcgt gacaggaatg cgaaacgggg
     4861 tgagagccac cggtgtgggc ggtcttacct tgatcgagat cctcgtcgtg gtggccatcc
     4921 tcggcgtgct ggcggcgctg atcgtgccgc gggtggtcgg ccgcaccgac gaggcgcgcg
     4981 cggtggcggc caagcaggac atcgccacga tcctgcaggc gctgaggatg taccggctgg
     5041 acaacgaccg ctacccgacc tcggcgcagg ggctggacgc gctggtcctg cggccgggat
     5101 ccgagcccat cccgcgcaac tggaagcaat acctcgacaa ggtccccaag gatccctggg
     5161 ggcagccgta tcagtacctg agcccgggga tacacggcga ggtcgatgtg ttctcgttcg
     5221 gcgccgacgg cacgacgggc ggcggtggaa tcgatgccga gatcggctcg tggcagctgt
     5281 gagcgcggct cgccccgcgg cgacgtgacg cgggtgcgcc ttgcgcgggc cctacgcggc
     5341 ttcactctga tcgagctgct ggtggcgatt acgatcctcg cgatcgccgc cgcgatcgca
     5401 gcgctgtcgg tcgcctccgg cgagggccgc agggtggacg aggagacgcg gcggttggcg
     5461 gccctgttcc ggctcgcgca agacgaggcg gcgatgtccg gaaccatgct ggtatgggaa
     5521 gccgacgtgc acggctaccg cttccgccgg ctcgaccgcg gcgacggcga aggcgagccg
     5581 tcgccgcagg gcgacctgtt gcgcgccagg cgctggccgt tcggcgtcag ccgtgtcgaa
     5641 gcgcaaccga tcgtgttcgg ccgggaacct ctgctccaac ccggcgtcat cagattgacc
     5701 accgcatcca gagatatccg gctcgcgctc gaccccttcg gcaacctcac ggaggcgcct
     5761 tgacccgctc ccgctgtgcc ggcttcacgc tcctcgaagt gctggtcggc ctggccatcg
     5821 ctgcggtcgc cctgatggcg tcgatccggg cggtcggctc gatggcgcag accaacgccg
     5881 cgctcgagct gcgcgcgctc gcgcaactgt ccgcgcacaa ccgcgtcgcg gaactgcgcg
     5941 caaccggcgc gttccccgcc gtcgggacgc gtaccgcccc ctgcccccag ggccgcacgc
     6001 aactgcagtg cgcggaagaa acccagccca cccccaatcc gctgttccga cgcgtggagg
     6061 tgcgggtcgc gcagccgtcc gcccccgacg ctgtgctcgc gcggctgacc gccgtcctgc
     6121 ctcgagtgcc atgagaccgc cgcacaggac gcgcgcgccc gggttcagcc tcctggagct
     6181 gctggtggcc atcgcgctgc tcggcgtgct ggcggtgctg ctgtggcgcg gactcggcgg
     6241 cgtcatcgca caacgcgaac gcatcgaccg cgatgcggcg cacgtggagc gcgtcattcg
     6301 cctgctggcg caactcgagc gcgacctcgc ccaacgcgca cccgatgtgg tgattcccgc
     6361 tggcgtcgaa gcgagcggcg gattgccgcg tgcgctggag gttcggcttc agcgcgaggg
     6421 caacgcccag ctcgtgatcc tgcgcaccgc gcccgacgcc ggcggcccgc tgcgcctgga
     6481 gcgggtgcgg tacttcgtcg cgcagggcgc gctgctgcgg gaagcttcgc cgccgagcag
     6541 cacctggccc ctggccccgc cgcccccgcc gctgcgcctg ctggacgcgg tggagcggct
     6601 ctcggtacgc ctgttgaggc tcgagccgcg cagcggcgcg ggtccttccg ccgccggtgc
     6661 gggcagcgat gcgcgcgtca ccggaatcga ggtgagcgtc gagcgcgcga acggggagcg
     6721 ctacgtgcgc gtggtcgcct tatgaaccac cgctcacagc gcggcggcgc cgtggtgatg
     6781 gcgatgctcg ctgtggcgat cgccgcgacg ctggtcgccg gcacgttctg gcggcaggag
     6841 gtatggctgc agcgagtgga gaacgagctg tcgttcgcgc aagcgcagtg gctgatgcgc
     6901 ggggtgatcg actgggcgcg cgtgatcctg gccgaagatg cgcgcacctc gcgggtggat
     6961 cacctgggcg agccgtgggc gctgcagatc gccgatacgc ggctggacga ctcgagcggc
     7021 acacccgcct acctcaccgg cggcatgcag gacgcccagg cgaagtacaa cctgcgcaat
     7081 ctggtggggc ccgacggcat cgtgcccgcc gagctggaag tgctgcgcag gctgctcgcc
     7141 ttcgccgagg tcccggaaac gctcgcgaac ccgatcgcgc agcgcgttct cgacggggtg
     7201 ccggccccgg ccggtgaagg cggcccggcc tcgaccggcg aaagccttgg ggcgcgtcgg
     7261 caggctttgc cgctgcgcac gctggacgac ctgctctcgg tgccgcagat cggcgttgct
     7321 caggtgcagg cgctgcgccc atatctcact gttctgcccc ggcccacgcc ggtcaacgcc
     7381 aacaccgcga gcgcccaggt gctggcgggc acgatcgccg acctgtcgct gccggacgcg
     7441 cgccgcctgc tggcgagccg cgaccgcgcg cacttcaagg acctgcacga cgttgtatcg
     7501 cgcatgtcgg gccggccgct gcaggccgat cccacgcggg tcgggatcga tacgcagttt
     7561 ttcctgatgc agggacacat cgtccatcgg cgcgcccggc tgcacgcgca ggcgctgctg
     7621 ctgcgcgccg gcaacagcgt gcagctcgtg tgggtgcgag agctgtaatg accacgctgc
     7681 gtgttttcct ctgtccgctc gccgagctcg gcgcgcagtc ggttctcgcc tacgagctgc
     7741 tcgatcgccg gcgccgcatc atgcgccgcg gacaggccgt gcccgcgctg ctgcccaaga
     7801 ccggccgctg cgaattggtg ctggccgcgc ccgacgtgct gctggtggac gccgcgctgc
     7861 cgaagatgag gggggcaagg ctgcgcgccg cgttgccgat gctggtcgag gaatttctgc
     7921 tcggggacat ggaggacgcg ctggtggtcg cgggcgctcc ggaccgcgcc agcgggcgcg
     7981 ccacggtggc gatgctcgac cgcgccctgc tgcagcgcgc gttgggcctg ttcgcgcaac
     8041 tgcgcattcg agtggtgagc gccacacccg agccgctgac gctggcggcc gcgcccgggt
     8101 gctggcgcgt gcgcctgcgc gaaagctacg gctacgcccg caccggcgag cgccttggca
     8161 tcgccctgtc gccagcgctc gacgcgggcc cgccgcggga gctcgcgctc gcgctggcgc
     8221 agcagccggt tcccagcgcg atcgaaatcg agggagaatg cgacgctgcg gcctgggagg
     8281 aggccttgcg ggtgccggtg cgcagggtgc gcgacgagca gacggtggct cccgcggtcg
     8341 cgctcgaact gctgcagtat gaagcggcac cggacgccgc cgactggtcg cgctggcgcg
     8401 ggccggtcgt cctcgccgcc gcgctcctgt tgttgtggat cgcggggctg aacgtgcagg
     8461 cctggctgct gcagcgcgag gagcgcgcgc tgcgtgcgga gatggcgaac ctggtgagac
     8521 aggcctttcc cgaggtgccg gtggtgctcg atccggtggt ccagatgcgc caaggggtcg
     8581 gcgaactgcg cgccgcggcc ggcatcgact cgcccacgga cttcctgccg ctcgccaccc
     8641 gtttcgcgca agcgcttgcc gacgaccggg cggtgcgctc gatcgagtac cgcgacgcgg
     8701 tgctgcacgt gcgcttcgcc gacgcgcacc tgctggagtc ggcgccggcg cgcctcgcgc
     8761 tctcctcgcg cctggcgcag gacggcctcg aggtgcaatt ctccaacgag ggcgcggtgg
     8821 tggcgcccag aagggtacgt tcgtgaggag ggtgcgctcg tgatcgcggc actccgcagc
     8881 tattggctcg gcttgtcgcg ccgcgagcgc acgctcagcg gggccgggat cggcatcgtg
     8941 ctgtgcgcgg ccgcgttcct ggtggcggtc gagccggcct ggaaaacgca ggcccggctg
     9001 cgcaccgaac tgcccgagct gcgcgagcag gcggcggaac tggccgcgct ggcgcaggag
     9061 gcggagcgcc tgcgcgagca tctcgaagcg cgcccgggga cggcggaact gaaggacgcc
     9121 atcgggcagt cgctgtcacg ggcggggctg gaggccgcca gcgtcgccgt cgtgggcgag
     9181 gggcagtgga ccgtgcgggc gcaggccgtt cccgccggac aatggctggg ctggctcgag
     9241 caagtcgcgc gcgagctgag gctgcgcgtg ggcagcgcgc gcattgcgcg cggcgatgcc
     9301 gtggggctga tcgacgtgga ggtgactctg aatgcgtttc ccaagcgctg aacgcagctc
     9361 ctggcggctg cgcgcgctgc agctgctcgc ggcgctggtg gtagtggccg ccatcgtgat
     9421 cgcgcggccg ccggccgcct ggctggcgga tctgctgcat gcgcacagcc gcctgcgcct
     9481 gatcgatccg cagggcacgc tctggcaagg ctcggcgcaa ctcgcggtga gcgacggccg
     9541 ccgcgcgctg ctggtgcccg gccggctcac ctggcgcgcg tccctggccg gctttctcgg
     9601 cgcgaaggtg gagatccgcc atcccgcgac gctggcgccg gtacgcgtga gcatcgaacc
     9661 gggcaaggtg gaaatcgagg cgggacacgc gcagacgccg gcggccgtgc tctccgccct
     9721 gggcacaccg ttcaacaccg tgcgcccggg gggcacgctg cacgtgcagt ggagcgcgtt
     9781 gcggctgcag caggaggcgg cgcacggcga cgtgcgcctc gagtggcgcg acgcgcagtc
     9841 cgcgctctcg ccggtcgccc cgctgggcga tttcctggtg gtcgtgaagc tgcgcggaga
     9901 ggccggcgag gtcgccctgc gcacgctgcg cggcccgttg cacatggaag gccgcggctc
     9961 gctcacccgg gggcagctcg ccttcaccgg gcaggcgttc gcggaagaac cgatgcgcgc
    10021 ggccctgctc gggctgatcg gtgtgctcgg caagcgctcc ggcgatcgcg cgcagctgcg
    10081 gctcggcacc tgaaaacgaa acgggaggtg atgcggatga acccgatgac cacgaaacgc
    10141 acgcggcgcg atggtttgct ggcgctggcg acccatgccg ttctcgcgct ttcgctcgcc
    10201 gtgccgctcc tcaccggcgg cgcgcagccg gcgaaggagg agaaggtgcg gctgcggttc
    10261 gtcaacgccg acatcgagtc ggtggtgcgc gccgtcgcgc atttcaccgg caagctcatc
    10321 gtggtcgacc cgcggctcaa gggcacgctg acgctgacct ccgacaagcc gatgaccagc
    10381 gctcaggcgc tcgccgcgct gcgttcggcg ctgcgggtgc agggtatcgc cgtcgtcgaa
    10441 tcggacgggg tctggcgggt ggtgccggag gccgatgcca aggtccagcc cgggccggtg
    10501 ctgagtcgcc cgacgcggcc gagcgcaggc gaccacatca tcacgcaggt gttccagctc
    10561 aagtacgagt cggcgcccaa cctgcttccg gtgctgcggc cgctggtcac cgcaagcaac
    10621 ccgatcagcg cctacgcggg caacaacacc atcgtggtga ccgattacgc cggcaacgtg
    10681 cggcgggtgg ccgagatcat cgcgtcggtc gacacgccca gcgccggggc gaccgaagtg
    10741 atccggctgc agcatgcgat cgccagcgac atcgccgtgc tgctcggccg gctgctcgac
    10801 gccggcggcg cccagggcgg cgacccttcg cagcgcgtca gcgtgcaggc cgagccctcc
    10861 agcaattcgc tggtgctgcg cgcgccgagc ccggcccgcg cggagcaggt caaggggctg
    10921 gtcgcgcgcc tggaccagcc tttgagccag gccaccggca acgtgcacgt ggtgccgctg
    10981 cgcaacgcgg aggcggccgc gctcgcgcgc acgctggccg gtctcgcccc ggccgattcc
    11041 ggcgccgcgc ccgactactc atcgtcgttc tcgggggcgc agcaacagcg cgcaccgggg
    11101 atggcgatgc tcgcgtccac gcagacgccg gcgcgaccgg cggaaggccc gatgccggtg
    11161 gccatgggcg gcctgcaggg gcaggcgatg gtgctcgcgg ataccgcgac caactcgctg
    11221 atcatcaccg cgccccagca gatctaccgg cagctgcggg cggtgatcga caagctggat
    11281 ctgcggcgcg cgcaggtgtt cgtcgagtgc ctgatcgcgg aggtcaccag cgatcaggcg
    11341 gccgagttcg gcatccagtg gcaggtcggg ctggacgaca tcgccggcag ctcgaccaac
    11401 gtggtcggcg gcacgaactt cggcggcgtc acccagaaca tcacctcggc ggcgcagaat
    11461 ctgagcctgc tggggccggg gctcaacgtc ggcgtggtga agggaaccat caccatcccg
    11521 ggcgtgggcg agatcaccaa cctcacggcg ctggcccggg cgctggagac ggtgaccaag
    11581 gccaacatcc tgtccacgcc cacgctgctc acactcgaca acgaggaggc gcagatcctg
    11641 gtgggacaga acgtgccgct ggtgaccggg cagtacgtca cgccggggac cggcggcccc
    11701 gtcacggtga acccgttcca gaccatcgag cgtcaggacg tcggtctcac gctgcgcgtg
    11761 cgaccgcaga tcagcgaggg cggcaccatc aagctggtga tctaccagga agtgtcgagc
    11821 ctgcagcggc gcacggtgga cacggcggat atcgtgctga acaagcgcac ggtggagacc
    11881 aaggcgttgg tggacgacgg gtccattgtg gtgctcgccg gcctggtgga ggacaacgtg
    11941 acctccaccc gcgagaaggt gcccctgctc ggcgaccttc ccctgatcgg cgcgctgttc
    12001 cgctacgaca ccaagcagca ggtgaaaacc aacctgatgg tgttcatccg gccggtggtg
    12061 gtgcgcgacg aggcggcctc gcggctgctg gccgccgacc gttacgatta catgcgcaac
    12121 ctgcagcagg tcacgccgtt gccggagcgc gggccgctgc ccgacgtgcg cgggccggtc
    12181 ctgccgcagc tggatggctc gggccggccg gcgcccgcgc cgtcgccgcc gcgcacctcg
    12241 tccccacggc cggggccggc cgcctataca cctccgccgg ggcggcgcta gaggcgcatg
    12301 gcgacgctca tcccttacgc ctttgcgcgg gcccacagcc tgctgccggc cggcgagcgc
    12361 gagggccgcc tacagctgtg ggtgacggag gcgacgccgc cggtggcggt gatggaggtg
    12421 cgccgcgccc tggcgcgcga cgtcgaaccg gtcatggtct cgcgcgagcg cttcgaggat
    12481 ggcctgcgcg cggcgtacgc acaggccgaa ggcgaggcag ccatggtggt gggagaggtg
    12541 cagagcgaga tcgacctcgc gcgtctgatg caggacctgc cgcaggtgga agacctgctg
    12601 gaagcgcgcg acgacgcgcc gatcgtgcgc atgctcaacg cgcttctgac gcaggcggtg
    12661 caggagcgcg cctcggatat ccacatcgag ccgttcgaga gctcctcggt ggtgcgcttc
    12721 cgggtggacg gcacgctgcg cgacgtggtc cgcccgcacc gcgcgctgca cgcggcgctg
    12781 gtctcgcgca tcaagatcat ggccgacctg gacatcgccg agaagcggct gccgcaggac
    12841 ggccgcatcc tgctgcgcct gggcggacgg caggtagacg tgcgcgtgtc gacgttgccg
    12901 gccgggcacg gcgagcgcgc ggtcctgcgc ctgctcgaca aggagcaggg acggctggag
    12961 ccggagcggc tgggcatgtc gcctgccgtg ctggccagcc tggagcaggt ggtgcggcag
    13021 ccgcacggca tcgtcctggt gaccgggccc accggcagcg gcaagaccac cacgctgtac
    13081 gcgatgctgt ccgcgctcga cgccgccacc accaacatca tgaccgtgga ggacccgatc
    13141 gagtacgatc tcccgggcat cgcccagacg caggtgaacc cgcgcatcgg gatgaccttc
    13201 gcgcgcgcgc tgcgcgcgat cctgcgccag gacccggacg tcatcatgat cggcgagatc
    13261 cgcgacctgg agaccgcgca gatcgccgtg caggcgtcgc tcaccggcca cttggttctc
    13321 gccacactgc acaccaatga ttcgatcgcc gcggtcacgc gcctgctgga catgggcgtc
    13381 gagccgttcc tgctgtcttc ctcgctgctc gccatcctgg cgcagcgcct ggtgcgccgg
    13441 ctctgcgtga gctgccgcac gcaggacagc aagggccaat ggcatccgct cggctgcccg
    13501 tcgtgccagg gcagcgggta tcgcggccgc gccggcgtgt acgagctgct gctcgtcgac
    13561 gatgccatcc gcgacgcgat tcactccggc gagggcgagc aggcgctgcg gcgcctcgcg
    13621 cagcagcgcg gcttccggcc gctgcgcgaa gacggcgagc gctgggtggc gagcggcgag
    13681 acttcgctgg aggagctgct gcgggtcgca agggagcaat gagcgcggct atgctgacgt
    13741 gcgcgagcgc ctgcaccgcc gtctcgagga cttgagccat gccctcctac cgcttcgaag
    13801 cctcggacgc ggcaggccgc gtcgagaagg gcgtgctgga agccgacggc gcgcgcgcgg
    13861 cgcggaccca gctgcgggcg cgcggcctgg cgccgttcgc gctcgagccg ctcgcaggcg
    13921 gcgccggcga aacgccgggc tgggtgcgcc tgcgactgcg cgagctcgag ctcaccaccg
    13981 ccacgcgcca catcgcggcc ctgctatcgg ccaagctgcc gctggccacc gcgctcacgg
    14041 ccgtcatcga gcaaagcgaa cggccggcca cccgcgaagc actctctgcc gtgcgcagcg
    14101 aggtggtcgc gggcgcccgc ttcgccgacg cgctcgcccg ttttccgcgg ctgttccccc
    14161 aggtctaccg ggcgacggtg gccgccggcg agcaggcggg cgatctggcg ctggtcatgg
    14221 aacgcctggc cgattacctg gagcagcgcc aagcgctgcg cggcaagatc ctgtccgcca
    14281 ttctgtaccc ggccgtggtc acgctggtcg ccgtggcgat cgtcttgttc ctgatgacct
    14341 acgtgcttcc gcaggtggtg caggtgttcg agcagacctc gcagtcgctg ccgttgccca
    14401 cacgcatgct gctcgccacg agcgccttcc tgcgcaactt cgggctatgg ctcgtgctgc
    14461 tgctgccggc gctcggcttc gcgctggccg cctggttgcg gcggccgggt cccagagcgg
    14521 cgtggcatgc gctgttgctg cgcctgcccc tgctcggctc ggtgctgcgc accgtggacg
    14581 cggcgcgctt cgccggcacg ctcgcgatgc tggcggcctc gggcgtgccg gtcctgcgcg
    14641 cgctcgaggg tgcgcgcgcg acgctgcgca attcggtgct ggcacaggcg gtctccgggg
    14701 cgatcgcgca agtgcgcgaa ggcgaggcgc tcgccagggc gctgggggcc acgcgcgcgt
    14761 tcccgccgat gctgatccat ctcatcgcca gcggcgaggc gaccggagag ctcgcccgga
    14821 tgctggatcg cgccgccgcg atccaggccc gggaagtcga acggcggctg ctggtgctga
    14881 ccaccttgct cgagccgctg ctgatcctgg cgatgggcgc gatggtgctc ggcatcgtgc
    14941 tcgcggtgct gctgccgatc atcgacatca atcagctggt gcgctgacgc gtcattcggc
    15001 gcaatcgctc agcgcgcgca actcgcttcc cgggaagcgg ctggcgagcg cggccagctg
    15061 cgcggacacc tgcgcgctcg gctcgcgcag gacgaacacg gtctgcgtga cacggtgctc
    15121 gcggctgccc attcgcgcca ggcgcacacc gcgggcctgc agcccgcgca ggaactcgcc
    15181 ggcggcctcc tcgctgcgga agatcccgag cgaaatcgcg ttgcgcatcg cgctctcccc
    15241 ttccaccgcg aaatagtcgg tcacgccgag cgcggtcagc tcggcgatgc ggcgatcgac
    15301 ttccacgcgg ctcacctgcg gcgggatgaa tacccaccag cccgccacca cgggcacgcg
    15361 cgattccgcc gcacgctgcg ccagtcccag ccgctccagc tcgcgccgcg ccgccgccag
    15421 ctcgacgtcg gagaaggtgc cccactccag gcacgcgctg cggcgcggcg ccggtggcgg
    15481 cggcgggatg atgcgcacct tgtccgcgtt gagctgctgc tcgagcagtg cggcgtcccg
    15541 gctgcgctgg ccggagaaca gcagcgcgta ggccgcgagc gccagattcg ccagcaccag
    15601 caccgtgaat accagtctca tgtgcgagcg atctcgatca agccctccag caccaggctt
    15661 ggcgcgagca ccgcgggatg ccgcagcagc ggcgcgacga gatgcgcgcc gccgccgctc
    15721 agcagggtcc gcgggtcgcc gtggccggcg tcgatcatcg cctgctgcat gcgctcgatc
    15781 gcgccgcagg tcgcctgcaa cgcgccgctg tggatcgcgt cggcggtgcc ggtcgggaac
    15841 ggctggaacc ggccgggctg ctgcggcaag ccggcggtcc cttgcgccaa cgcttgcgcc
    15901 atcagatcgg gaccgggcag gatcaagccg cccaggaagc gcccgtccgc ggtgagcgcg
    15961 tcgatggtga ccgcggtacc gacgttgacc acgacgcagg cgccgtcgcg ccgcgcgtgc
    16021 gcccccacca gcgccgccca acggtcgctg cccagttgcc ccggcgcggc gtagccgctc
    16081 accacgccgc actcgctggc ctggctgctt atccagcgca cggcgatccc ccagggggca
    16141 agcgcctgct cgatctgcgc cctggcgtgg tcaccggcga cgctggagcc gaccgcctgc
    16201 tgcggccgag gcagcatgtg cagggcatcc gccaagtcgg ccgcgtgtgc cgtgagcacc
    16261 gatccctgag ccacgaagcc acgctcctgc cacagccccc acttgatgcg cgtgttgccg
    16321 gcgtcgatgg cgaggatcaa cctgccctcc gtaagccccc ttccagtggc cgcaggctca
    16381 actctcccga gtggaagcgg cgcagcccgg cggtggtctg cagctgcaac gcgccgtcgt
    16441 cggccacgcc ggcggcgatg ccggcatgct cggtgcgatc gggcatgatc gccgccaccg
    16501 cccgcccggc gagcgcatcg tagcgcatcc attcgtcctt gagcggggcg aagccgtgcg
    16561 cggcgaacag ctccagcgca tccgccacgt gccgcagcag ggcgcccagc acccggttgc
    16621 gatcctgccg caccccggcc gcggtcagat cggtcaccgc ctggtcgatg cgctggcgca
    16681 cgccggggtc cacgcgcacg ttgacgccga tgccgatcac cgccgcgctc ggccccatca
    16741 catcgccctc gagctcggtg agcacgcccc cgagcttctg gtagcggcac agcagatcgt
    16801 tcggccattt cagctgcact tcgtcgccgc ccagctcgcg caacgcacgc accagggcga
    16861 cgctcacggc aaggctcagc ccgcccaggt tgcccacccc ctgctcgaag cgccacaaca
    16921 tcgagaacgt cagtccgccg cccagccccg agtgccagcg gtgtccgcgc cggccgcgcc
    16981 cgcctgtctg cacctcggca gcgagcgcga ggccgctcgg cgcaccggcc aacgcctcgg
    17041 cgagcagcag cgtgttggtg gaatcgaccg cgtccacgac gtgcacctga aaagtctccg
    17101 cgcgcgcgcc cagatggcgg cgcacctgcg cagcatcgag ccagtccatc ggagtcacca
    17161 gccggtagcc gcgtccgtgc accttgaaca gcgtgacgcc gaacgcttcc agcccctgta
    17221 gcgccttcca cacggtggtg cgcgatacgc ccagcgcgcg cgcgagcgcc tccccggagt
    17281 ggaagtcgcc gtcggacagc aggcgcagcg cgcggaacgc aagcggagcg ggagaggcgg
    17341 tcatggcggg aacgtaggct cgaagcaggc ggccggggaa gctaaaccat cggcggccgg
    17401 tttgtcaaag ccggcctgcc ggtatactgc gctttgacct gggtcaagcg cgccgatgcg
    17461 ctttactgga acactgccgc caggcgcgac cgcgcagcgc tttcggatcc gccgcttcgg
    17521 atccactggg gaggagacat gttcaagaag atcctgctcc cgaccgacgg gtcggacctg
    17581 tccaagaaag ccgtcaagaa gggcgtcgac ttcgccaagt cgatcggcgc cggcgttgtc
    17641 ggtttcttct cgccggagga ctaccgcgcg ttgatgtaca gcgagtacat cccgcccagc
    17701 ctgctgtccc aggaggagtt cgaggccaac gccaagaaag cggcggagaa gcacctcgcc
    17761 ttcatcgaga agacggcggc ggccgccggg gtgccgtacg agggctactt cctgtccagt
    17821 atcgcgccct gggaggcgat cgtcgaggcc gcgaagaaga agcgctgcga cctgatcttc
    17881 atggcctccc acggccgcac cgggctggcc ggactggtgc tcggcagcca gaccaccaag
    17941 gtgctcacgc actcgaagat cccggtgttg gtgtaccgct agccaccgga ttcgctgcgt
    18001 cgttgtgcct cgcccggcag cgacgcgccg accctggccg gcgcgaggaa ccagctgtcc
    18061 ttgggcacga ctttcggtcg ctcgggatcg ccctcgtagg ccggcgtcat gatctgcacg
    18121 ccgtactcgt tgaacacgtc caggatgttc cgatgcagct ccgtgtacag acgcgccatc
    18181 gacgacggcg tgtcgcagaa gacgttgagc tcgtaggtga tgcagaagtc tcccagcgcc
    18241 ttctgcagca cgaagggagg cggctcggcc aacagcccgg cggtgcgctg cgcggccatc
    18301 agcagcatcg cctccacctg cctccagggc gtctcgtagc cgatgcccac cgtagtgtgc
    18361 aggatcagac cacgctcccg agcgagcgtg ctgtagttga tcacgttgcc gttgagcagg
    18421 atcgagttgg gcaccacgat ttcctcgttc ttgggcgaac gcaaatgtgt cacctgcagc
    18481 cggatctcgg tgacgtcccc gatcagcccc tccacctgga tgcgatcgcc gacacggaac
    18541 gcgcgccggt aagtcatggt gtagccggcg atgatgttgc cgatgaccga ggtcgagccg
    18601 agggagaaca tcacaccgag gaagatcgag acgcccttga atgctgcgga ctccgatccc
    18661 gggatgtatg ggtaggccac cacgacggcg aacgcgacga ccagcaggcg gacgatgcgg
    18721 taggtcggca tcgcccactc ccggtcgaag tcccgcagca ccaccgtgcc atgctccact
    18781 gccgcgaaga acaagtgcag gagtctgagc aggtagcggg tcaccacggc caggacggcg
    18841 aggaatacca ggctgggcaa ggccgagagc aggctgctgc cgatcgaccc cagcggtccg
    18901 agcaccaggc tgagcagacg ctcgccgaac actcgcgtcg ccggatactg gctcaggatg
    18961 aactgcaggc acaggtatgc cagaacgagc accgccagca gatgcagaaa gctcaagccg
    19021 cttcgcagtc cctgccacag ctgctcggcg cgcagcagcc gaaaagactg tatgccgagg
    19081 tcgtgaacgc gcgcgcggta tcgcgcctcc agcacggctg ccaggcgccg aaacggccag
    19141 cgcagcgcga gcactacggc ggcgagcgcg gccagcgccg ataccgagta cacggtgctc
    19201 atcagcagtg cgcgcggctc gcggtcgcgc ctgtagcgga cgaccgcctc cgcaatcgca
    19261 tcgcgatgca agccggccag ccgctggcgc tgcacgcctt cgatgctggc atcggcctcg
    19321 accaccgcca tgatccggcg ctctccggcc acgatcatcg acatgtcgcc ggactcgacc
    19381 acacggatcg cgccggcagg aatggcgcgg tctgcggcgg cttcctcgac gcgcgcttcg
    19441 atcctggcgg cgcgttgctc aggggggaat gatggactgc ctcgcacccg caacagcgtc
    19501 ctgccgtcga gcgccacggg cgcggtgggc cagtgcgctt cccgctccgc gctctcgaag
    19561 cccgacacgg gtgctgtggc gccggggaca tcgggccggg tcgcggtcgc ggcgggctcc
    19621 tcgcctcgcg cgtcctgcgc cagcccggca agcgcgagga tagcaaggaa cgcgacggtc
    19681 gccgcccagt tggcggtcaa gacggcggcc ctcgggccgc cggcaggttc gagcgcaagg
    19741 tggctcaaaa gtgtcccttc acgtagaaca gcagaccgct gaagtcgagc ttcactttgc
    19801 cggcgaaatc cacgccgtag tcggtgtcct cttcggcctc caccatgtaa cgcacggca
//
LOCUS       DASPGE010000218        11506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_995427, whole genome shotgun sequence.
ACCESSION   DASPGE010000218 DASPGE010000000
VERSION     DASPGE010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11506
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995427"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VD791_07035"
     CDS             complement(<1..889)
                     /locus_tag="VD791_07035"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXV07764.1"
                     /translation="MQRSRPQHEPARQRPPHRRPALRRLLLWLSGTLGGVIVVALAAL
                     LSAPLWLNADAVKARLLAQLQRNVPGELAYRDLELELFPRPGVTLADLRWALPGRGEA
                     QAAQAEVRLAWLPLLAGDVRIGVVRLRSPRVVLILQQAPPDGEELTAALVDARLRNAL
                     HRLAELAPGMDLEIADGELDLRPAQGPALVLRNLDVALASRPNRIELSVRATSEPVAR
                     LTGRLRLAHAALEGGIELGLGGVRLAALQGFLPWQPDVYGIDGEVDVRLLGTMRGASQ
                     LSVELDASAARLQLTAAGKP"
     gene            complement(1050..3269)
                     /locus_tag="VD791_07040"
     CDS             complement(1050..3269)
                     /locus_tag="VD791_07040"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXV07765.1"
                     /translation="MERTEISNSSVPISARAGRLADLMGLWADAVRIGERTLGPLADL
                     AVRLWLAQIFFVSAVLKVSNWDNALFLAEHEYPVDWLDPATAAWLGAATELAGSILLA
                     LGLATRCAAAPLLVLSLVIQFEYHALDAHLFWAALFLWYLVRGAGPLSLDAMLARGLA
                     ATPLPIVPALLRLAAALSRRAAPVLQLALRLWLAAALAIEVAMLAAGAVVRAWLPVES
                     AAHFPFPVAVAGVLLLVPGFATRLTALGMMVALLGVRTMGAEAVGSGYWLLALALLAL
                     HGPGRWSVDALIEAALKCMFPQLDGKPAFSLEGLPRVVIVGAGFGGLACAAGLRSAPL
                     RVTLIDRHNYHLFQPLLYQVATSSLSAGDIATPVRGLFRDQFNTDVIYGEVTGVDSAR
                     REVIVGAKRIAYDYLVLATGASHSYFGKDEWAPFAPGLKRIEDAIQVRAWLLSAFERA
                     EASDDALERRGLLNFLIVGGGPTGVELAGAIAELARFGMDQDFRHFDPAQARVILVQA
                     APRLLPTFPEALSEEAKRSLERLGVEVLVGSRVEKIDAAGVTVNGQRIPSRTVLWAAG
                     VVASPAARWLGVQADNAGRIEVGADLSLPGLPDVFAIGDTALSSAWDGQPVPGLAPAA
                     KQGGTYVARVIRARIAGKPAPAPFRYRHLGSLATIGRKAAVADFGWVRLSGASAWWLW
                     GLVHVGFLVGLRNRVSVMLDWAWSYLTYRSGTRLITGAAHSDQAGLAAAAAVMQRAA"
     gene            complement(3336..4169)
                     /locus_tag="VD791_07045"
     CDS             complement(3336..4169)
                     /locus_tag="VD791_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HXV07766.1"
                     /translation="MERIDTESLTAAPAWLAGARRALATHWPEYLMEAAGLGLFMVSA
                     CMFVALFEYAGSPARHWIEDAFVRRLPIGVAMGLTAVALIYSPWGKRSGAHLNPAVTF
                     TFWRLGKIASWDAALYVGAQFAGGTLGVLLSASVLTPQVIAHASVNYAVTQPGAQGVA
                     VAFAAEAAISFGLMLAVLIVSNRPSLNRYTGLFAGALVATYIGIEAPLSGMSMNPARS
                     FASAFPAQIWPGLWLYFVAPPSGMLLAAEAYLRARRPVFCCKLHHDNDQRCIFHCGYP
                     H"
     gene            complement(4294..7008)
                     /locus_tag="VD791_07050"
     CDS             complement(4294..7008)
                     /locus_tag="VD791_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HXV07767.1"
                     /translation="MKTALNAERQRLEAQHEGREDWRLWGPYLAERAWGTVREDYSPY
                     GNAWEDFDHDQARSRAYRWNEDGLGGISDAQQRLCFALALWNGRDPILKERMFGLTGN
                     QGNHGEDVKEYWFYLDATPSHSWLRYLYKYPQAEYPYARLVEENRRRSRQDPPFTLLD
                     TGVFEEGRYFDVEARYAKASPEEIHIRIVATNRGPDPAPIHLLPMLWFRNTWSWGDAA
                     DKPALRAIPAPKGAKWAVQAEHPSLGAYYLYGRHDAQPLYTENESNAQRLWGQPNASP
                     YVKDACQRRVIDGDESAVNPAMTGTKFAAWHTLTVDPGHSVTIGMTLSAQPLAAPFDK
                     REVIFAKREAEAAVFYDELLPEADPQDMNILRQALAGMIWSKQFFHFDVSRWLDGDQL
                     APPQSRKWGRNRRWRHVHAGDVISMPDKWEYPWFAAWDLAFHCGALALADVDFAKDQV
                     ELLLNERYLHPNGQIPAYEWSFDDVNPPVLAMAALKAFRAERVQRGRGDIGFLQRVTH
                     KLLMNYTWWLNRKDADGNNVFEGGFLGLDNISVYDRSQPLPPGFSLKQADATGWMAMF
                     ALNMTVMCLELAASDSDYEDVAIQCYLQFLGIAKTIAGGSGMGISLWDEQDGFFKDVV
                     VGPDGSAQRIDVFSWVGLIPLFACEIVDRRLLAASPRFERVMKMHRYGLFDGMYVSEC
                     PTKTNARGEHLLALVDADKLSRVLQHLLSEREFLSAYGVRSVSRIHAERQDLGHVHGV
                     GRALIEYVPGESTSGLFGGNSNWRGPIWMPTNYTLVQAIEKFHRFLGDDFTVPVPCLG
                     DAPLNLNQIAKLISDRLVDIFRRGPDGRIPALPADSPFQRDPAWRDLLLFNEYFHGET
                     GLGLGAMHQTGWTGLVANLVQRRYRGDIPAFWKRQTKGQPQQAAEVTA"
     gene            complement(7047..9119)
                     /locus_tag="VD791_07055"
     CDS             complement(7047..9119)
                     /locus_tag="VD791_07055"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HXV07768.1"
                     /translation="METRLPDLIRFGRDICGDLAQAERREWWLSNGLGGFAAGTVAGT
                     LTRHYHGLLVAPVQPPLGRHLVVAKADATLIDGERSWALATNRWSGGAVQPAGWLNIE
                     SFRLDGRIPVWRYACGELRVEQRIWMQYGESTTYVAWRLAPDSTPPQRPPELRVKLLV
                     NARDFHGTARPWDFNPVIESSGGSEGSGSELRVVHPNWFSAVLKVRGGGIATRHDWYE
                     NFDLALERDRGLPDRDCHLCVGEAQLILHPGQWVGVVASLEANASVYLDEALRRAQAR
                     DVGQLRRAQVQAPELLGAPQWIDQLVLAADSFVFARPLPEVPDGESVIAGYPWFGDWG
                     RDTMIALPGLTLAVGRFDTARRILETFARFVDGGMLPNVFPGAGDKPDYNTVDAALWY
                     VEAWRAYIDALGDMASLKRVFPILASIIDRHLRGTRYGIGVDATDGLLRAGEEGVQLT
                     WMDAKVGDWVVTPRIGKPVEINALWFNALCVMADFAERLGQAAPYRELAERARNGFGR
                     FLRPDDDGLYDVIDGPGGEDDALRPNQIFAVSLPHSPLAAQTQAQVLRAVGRELLTSY
                     GLRSLTPAHRDYRARYEGGVWERDGAYHQGPVWAFLLGHYALADYRVRGDPGAAQALL
                     EPLRDHLLDAGLGTVSEIFDGAPPHTPRGAPAQAWSVACTLEAWWRLERAKRSAMRRD
                     ALSEQKAA"
     gene            complement(9155..10618)
                     /locus_tag="VD791_07060"
     CDS             complement(9155..10618)
                     /locus_tag="VD791_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HXV07769.1"
                     /translation="MKYDVIVIGSGAGGSAAAYHLTQTGRRVLLLEKGHRLPADGSTL
                     DVDTVMRRGAFLADEPWLDRNANVTTPEEHFNLGGKTKWYGAALLRFAPREFEPDPAR
                     GFLPWPIAYEELAPFYEEAERLLAVRTFTVEADLRRLAGGLRRLDAGWSTQTLKLGLD
                     AGILDHPQEVRHFDGFASARGLKSDAERCLLDRVRSRPNLEILTGKAVRTLLPAPHGA
                     IRVGGAECEDGTRYHADTVLLAAGALHSPRLLQRYLEATGLAASLPAYRNVGRNYKFH
                     VLTAMLAFSPRRVRDVLGKTLLLTHTGFAHSTVQALGGNLACEILRVQAPALAPRPLV
                     EPFAQRALGLFLQTEDGSHPDNRILAAAGSPPRPQIDYDPARLPEAYAEHAALVSTLR
                     RQLLRLGYVSVARAIPISGTAHACGTLVAGTDPTRSVVDAVGRVHGMDNLYVVDGSAL
                     PRSSRVNPALTIYAWALRVASLLDSRAAPRRAAAAVQ"
     gene            complement(10680..>11506)
                     /locus_tag="VD791_07065"
     CDS             complement(10680..>11506)
                     /locus_tag="VD791_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV07770.1"
                     /translation="AGPDQDALTGIFGTRVRMARLQLGDEFVELEQYVAAAGQPIPLD
                     SRSNDLWFQHFAVVVSDMEKAYEHVRGFGVQSISSAPQTIPASNRAAAGIKAYKFKDP
                     DGHPLELLYFPPDKGRAKWQDKSRLFLGIDHSAITVADTERSTGFWRDLVGLAVAGGS
                     LNSGTTQEQLDNAFGAVVRITGLKPERDASPGLEFLQYLTPSGGRPAPIGLRANDLVA
                     TRTIVEVDDLAALADKLQAQKASFISPQPVSVSGMPWRKGLMVRDPDGHAVLLVEP"
BASE COUNT         1827 a         4045 c         3979 g         1655 t
ORIGIN      
        1 gcggctttcc cgcagcggtg agctggaggc gcgcggcgct ggcgtcgagc tcgacgctga
       61 gctgcgacgc gccacgcatc gtaccgagca ggcgcacgtc cacctcgcca tcgatgccgt
      121 agacatccgg ttgccagggc aggaaaccct gcagcgcggc gagacgcacg ccgccgaggc
      181 cgagctcgat tccgccctcc agcgccgcgt gcgccagccg cagccggccc gtgaggcgcg
      241 cgaccggctc ggaggtcgcg cgcacgctca actcgatccg attcggtcgc gacgcgagcg
      301 ccacgtcgag attgcgcagc accaacgccg ggccctgcgc tggccgcaga tccagctcgc
      361 cgtcggcgat ctccaggtcc atgcccggcg ccaactcggc caaccggtgc agcgcgttgc
      421 gcaagcgcgc atcgacgagc gccgcggtga gttcctcgcc gtcgggcgga gcctgctgca
      481 ggatcagaac cacgcgtgga gagcgcagcc gcaccacgcc gatgcgcaca tcgcccgcga
      541 gcagcggcag ccacgctagg cgcacttcgg cctgcgcggc ttgtgcttcg ccgcggccgg
      601 ggagcgccca gcgcaggtct gcgagcgtca cgcccgggcg cggaaacagc tccagctcga
      661 gatctcgata ggcgagctcg cccggcacgt tgcgttgaag ctgcgcgagc agccgcgctt
      721 tgaccgcgtc ggcattgagc cacagcggcg cggacagcaa ggccgcgagc gcgacgacga
      781 tgaccccgcc cagcgtgccg gacagccaca gcaggagacg ccgcagcgcg ggcctgcggt
      841 gcggcgggcg ttggcgcgca ggttcgtgtt gcggtcggct acgctgcact ccccacatcg
      901 gacgggtcgc tctcgcatgc gaatcccctc ctgcggcaaa ccctgctccc gacagcgatc
      961 gagatccgac gccgcagggt agcagacttg cgcggcgtga gcgccgatgc cggctcggct
     1021 atttcatcag gctaccccgc atcgcaccgc tacgcggcgc gctgcatgac tgcggccgcg
     1081 gccgccaggc cggcctggtc cgaatgcgca gcgcctgtga tcagccgcgt gccgctgcgg
     1141 taagtgagat aggaccaggc ccagtcgagc atcaccgaca cgcggttgcg cagcccgacc
     1201 aggaagccca cgtgcaccag gccccagagc caccaggccg atgcaccgct caagcggacc
     1261 cagccgaaat cggccaccgc cgccttgcgg ccgatggtag ccaggctgcc cagatggcga
     1321 tagcggaacg gcgcgggcgc cggcttgcct gcaatccggg cgcggatgac tcgggccaca
     1381 tacgtgccgc cctgcttggc cgccggcgcc agccccggca ccggctgacc gtcccaggcg
     1441 ctgctcagcg cggtgtcgcc gatggcgaag acatcgggca gcccgggcag cgacaggtcg
     1501 gcgcccacct cgatgcgccc ggcattgtcg gcctgcacgc ccagccatct ggccgccggc
     1561 gatgccacca cgcccgcggc ccacagcacc gtacgcgagg ggatgcgctg cccgttcacc
     1621 gtcacacccg ccgcgtcgat cttttccacc cgactgccga ccaggacctc cacgcccagc
     1681 cgctccagcg aacgcttggc ttcctcggac aacgcttcgg ggaatgtcgg cagcagccgc
     1741 ggcgcggctt gcaccaggat cacgcgtgcc tgcgccggat cgaaatggcg gaaatcctga
     1801 tccatgccga aacgcgccag ctcggcgatg gcaccggcca gctccacgcc ggtcggcccg
     1861 ccgcccacga tcaggaagtt gagtagaccg cggcgctcca gggcgtcgtc gctggcctcg
     1921 gcgcgctcga aggcgctgag cagccacgcg cgcacctgga tcgcgtcttc gatgcgtttc
     1981 agtcctggtg caaacggggc ccactcgtcc ttgccgaagt agctgtgcga tgcgccggtg
     2041 gcgagcacca gatagtcgta ggcgatgcgc ttggcgccca cgatcacttc gcggcgcgcg
     2101 ctgtccacgc cggtgacttc gccgtagatc acgtcggtgt tgaactgatc gcggaatagg
     2161 ccgcgcaccg gcgtggcgat gtcgccggcc gacaggctgc tggtggccac ctggtagagc
     2221 agcggctgga acagatggta gttgtgccgg tcgatgagcg tcacccgcag cggtgcgctg
     2281 cgcagccccg ccgcgcaggc gaggccgccg aagccggcgc ccacgatcac cacccgcggc
     2341 aagccctcga gcgagaacgc cggcttgccg tcgagctggg ggaacatgca cttgagagcg
     2401 gcttcgatca gcgcatcgac cgaccagcgg ccgggcccgt gcaacgccag cagcgcgagc
     2461 gccagcagcc agtatccgga gccgacggcc tcggcgccca tcgtgcgcac cccgagcagc
     2521 gcgaccatca tcccgagcgc agtgaggcgc gtggcgaagc ccggcaccag caacagcacg
     2581 ccggctaccg cgaccggaaa cgggaaatgt gcagcggatt ccaccggcaa ccacgcgcgc
     2641 acgacagcac cggccgcaag catggcgacc tcgatcgcca gcgcggcagc cagccacagg
     2701 cgcagcgcga gctgcagcac cggggccgcc cgccgcgaga gcgctgccgc gagccggagc
     2761 agggccggca cgatcggcag cggcgtggcg gccagcccgc gtgcgagcat cgcgtcgagc
     2821 gacagcggtc ccgcgccgcg caccaggtac cacaggaaca gcgccgccca gaacagatgc
     2881 gcatcgagcg cgtgatactc gaactggatc accaggctca gcaccagcag cggcgcggcg
     2941 gcgcagcgtg tggcgagccc gagcgcgagc aggatcgagc cggcgagctc ggtggccgcg
     3001 cccagccatg ccgcggtcgc cggatcgagc cagtccaccg gatactcgtg ctcggcgagg
     3061 aacagggcgt tgtcccagtt cgagaccttg aggacggcgg acacgaagaa gatctgcgcc
     3121 agccacagcc gcacggccag gtcggcgagc ggaccgagcg tgcgttcgcc aatgcgtacc
     3181 gcgtcggccc acaggcccat gaggtccgcc agccgcccgg ctcgggcgct gatcggtacg
     3241 gacgagttgg aaatttccgt gcgttccatg tcgactccgg atggtcgaga cgcgtagccc
     3301 ggatgcagcg cagcggaatc cggggatgaa gagcgctaat gcggatagcc gcagtggaag
     3361 atgcagcgct ggtcgttgtc gtggtgcagc ttgcagcaga acaccggccg ccgcgcgcgc
     3421 aaataggctt cggcggcgag cagcatgcct gatggcggcg ccacgaagta cagccacaag
     3481 cccggccata tctgcgcggg aaacgcggaa gcgaagctgc gcgcgggatt catgctcatc
     3541 cccgacagcg gcgcttcgat acctatgtag gtggccacca gcgcgcccgc gaacagtccg
     3601 gtatagcgat tgagcgaagg acggttggag acgatcagta ccgccagcat caaaccgaag
     3661 gagatcgccg cctccgcggc gaacgccacc gccacgccct gcgcgccggg ctgcgtcacc
     3721 gcgtagttga cgctggcgtg cgctatcacc tgcggcgtga gcaccgacgc cgacagcagc
     3781 acccccaggg tgccgccggc gaactgcgcg ccgacgtaga gcgcagcgtc ccagctcgcg
     3841 atcttgccga gccgccagaa cgtgaacgtc accgccgggt tgagatgcgc gcccgagcgc
     3901 ttgccccagg gcgaatagat cagcgcgacc gcggtgagtc ccatggcgac gccgatcggc
     3961 agacgccgaa cgaaagcgtc ttcgatccaa tggcgcgcag gcgagcccgc gtactcgaac
     4021 agcgccacga acatgcacgc cgagaccatg aacaatccca gcccggcggc ctccatcagg
     4081 tactcgggcc agtgggtagc cagtgcgcga cgcgcgccgg ccagccaggc cggcgccgct
     4141 gtcagggatt cagtgtcgat tcgttccatg cttcgatgct catttgcgtg tacaccacac
     4201 cgccccgccg gctgcgccgc accccgcctc gtgagaggcg gggaatggtc ttttaagacg
     4261 cgccttcagg gcgcattgag cgtggtacgc gaatcaggcc gtgacttcgg ctgcttgctg
     4321 cggctgaccc ttcgtctgcc tcttccagaa cgccgggatg tcgccgcgat agcggcgctg
     4381 caccaggttc gccaccagcc cggtccagcc ggtctgatgc atcgcgccca gccccaatcc
     4441 ggtctcgccg tggaagtact cgttgaacag cagcaggtca cgccacgccg ggtcgcgctg
     4501 gaacgggctg tccgccggca gcgccgggat gcggccgtcg ggcccgcggc ggaagatgtc
     4561 caccagacgg tcggagatca gcttggcgat ctggttcagg ttgagcggcg catcacccag
     4621 gcaggggacc ggcacggtga agtcgtcgcc caggaagcgg tggaacttct cgatcgcctg
     4681 caccagcgtg tagttggtcg gcatccagat cggtccgcgc cagttcgagt tgccgccgaa
     4741 caggccggag gtggactcgc ccggcacgta ctcgatcagc gcgcggccga cgccgtgcac
     4801 gtgcccgaga tcctggcgct cggcgtggat gcgcgacacg ctgcgcaccc cgtaggcgga
     4861 caggaattcg cgctcggaca gcaggtgctg cagcacgcga ctcagcttgt ccgcatccac
     4921 cagcgccagc aggtgctcgc cgcgcgcgtt ggtcttggtc gggcattcgg acacgtacat
     4981 gccgtcgaac aggccgtagc ggtgcatctt catcacgcgc tcgaagcgcg gcgaggcggc
     5041 cagcagccgc cggtccacga tctcgcaggc gaacagcggg atcaggccga cccaggagaa
     5101 cacgtcgatg cgctgcgccg agccgtccgg ccccaccacc acgtccttga agaagccgtc
     5161 ctgctcgtcc cacagcgaga tgcccatgcc gctgccgccg gcgatggtct tggcgatgcc
     5221 gaggaactgc aggtagcact ggatggcgac gtcttcgtag tccgagtcgc tggcggccag
     5281 ttccaggcac atcaccgtca tgttcagcgc gaacatcgcc atccagccgg tggcgtcggc
     5341 ttgcttcagg ctgaacccgg gcggcagcgg ttgcgagcgg tcgtacaccg agatgttgtc
     5401 caggccgagg aagccgcctt cgaacacgtt gttgccgtcg gcgtccttgc ggttgagcca
     5461 ccaggtgtag ttcatcagca gcttgtgggt cacgcgctgc aggaagccga tgtcgccgcg
     5521 gccgcgctgc acccgctcgg cacggaaggc cttgagcgcg gccatggcga gcaccggcgg
     5581 gttgacgtcg tcgaaggacc actcgtaagc gggaatctgc ccgttgggat gcaggtagcg
     5641 ctcgttgagc agcagctcca cctggtcctt ggcgaagtcc acgtcggcca gcgccagcgc
     5701 gccgcaatga aaagccagat cccaggcggc gaaccagggg tactcccact tgtcgggcat
     5761 ggagatgacg tcgcccgcat gcacgtgccg ccagcgccgg ttgcggcccc acttgcgcga
     5821 ctgcggcggg gcgagctggt cgccgtccag ccagcgcgac acgtcgaagt ggaagaactg
     5881 cttgctccag atcatgccgg ccagcgcctg gcgcaggatg ttcatgtcct gcggatcggc
     5941 ctcgggcagc agctcgtcgt agaacaccgc ggcctcggcc tcgcgcttgg cgaagatcac
     6001 ctcgcgcttg tcgaacggtg ccgccagcgg ctgcgcggac agcgtcatgc cgatggtcac
     6061 ggaatggccc gggtccacgg tgagcgtgtg ccaggcggcg aatttggtgc ccgtcatcgc
     6121 ggggttcacc gcactttcat cgccgtcgat cacgcggcgc tggcaggcgt ccttcacgta
     6181 aggcgaggcg ttcggctgcc cccacaggcg ctgcgcattg ctctcgttct cggtgtacaa
     6241 cggctgcgcg tcgtggcgtc cgtacaggta gtaggcgccc agcgacggat gctcggcctg
     6301 taccgcccac ttcgcgccct tgggcgccgg gatcgcgcgc agcgccggtt tatctgcggc
     6361 atcgccccag gaccaggtgt tgcggaacca aagcatcggc agcaggtgga tcggcgccgg
     6421 atcggggccg cggttggtcg cgacgatgcg gatgtggatc tcctcgggcg aggccttcgc
     6481 gtagcgcgcc tcgacgtcga agtagcgccc ctcctcgaac acgccggtat cgagcagcgt
     6541 gaacggcggg tcctgacgcg agcgacgccg gttctcctcg accaggcggg cgtacggata
     6601 ctcggcctgc ggatacttgt agagataacg gagccagctg tgcgaaggcg tggcgtcgag
     6661 atagaaccag tactccttca cgtcctcgcc gtggttgccc tgattgccgg tcaggccgaa
     6721 catccgctcc ttgaggatcg ggtcgcgtcc gttccacagc gccagcgcga agcacaggcg
     6781 ctgctgcgca tccgagatgc cgccgaggcc gtcctcgttc cagcggtagg cgcgcgagcg
     6841 ggcctggtcg tggtcgaagt cctcccaggc gtttccatag gggctgtagt cctcgcgcac
     6901 cgtgccccag gcgcgctcgg cgagataagg accccacagg cgccagtcct cgcggccttc
     6961 gtgctgcgcc tcgagacgtt gccgctccgc gttcagtgct gttttcatcg aatacgtcct
     7021 gtgtgtcgac tgcgtttcgt ggtagttcaa gccgccttct gttcgctcaa ggcgtcgcgc
     7081 cgcatcgcgc tgcgcttggc gcgttccagc cgccaccagg cctccagcgt gcacgccacc
     7141 gaccaggcct gggcgggcgc gccgcgcggc gtgtgcggcg gcgcgccgtc gaagatctcg
     7201 ctcaccgtgc ccagcccggc gtcgagcagg tgatcgcgca acggctcgag cagcgcctgc
     7261 gcggcaccgg gatcgccgcg cacgcggtag tcggcaagcg cgtaatgccc cagcaggaac
     7321 gcccagaccg ggccctggtg gtaggcgccg tcgcgctccc acacgccgcc ttcgtagcgc
     7381 gcacggtagt cgcgatgggc cggcgtgagc gagcgcagtc cgtaggaggt gagcagctca
     7441 cgccccaccg cccgcagcac ctgcgcctgc gtctgcgccg cgagggggct gtgcggcaga
     7501 ctgacggcga agatctgatt gggccgcagc gcgtcgtcct ctccgcctgg tccgtcgatg
     7561 acgtcgtaca gaccgtcgtc gtcggggcgc aagaaacgcc cgaagccatt gcgcgcccgc
     7621 tcggcgagct cccggtacgg ggccgcctgc cccagccgct cggcgaagtc ggccatcacg
     7681 cacagggcgt tgaaccacag cgcgttgatc tccacgggct tgccgatccg cggcgtgacc
     7741 acccagtcgc ccaccttggc atccatccac gtgagctgaa cgccctcctc gccggcccgc
     7801 agcaggccgt cagtcgcgtc cacgccgatg ccgtagcgcg tgccccgcag atggcggtcg
     7861 atgatcgacg ccagaatcgg aaacacgcgc ttgagcgaag ccatgtcgcc gagggcgtcg
     7921 atgtaggcgc gccatgcctc gacataccac agcgcggcgt cgaccgtgtt gtagtccggc
     7981 ttgtcgcccg cccccgggaa cacgttgggc agcatgccgc cgtccacgaa gcgggcgaac
     8041 gtctccagga tgcggcgcgc cgtgtcgaag cgcccgaccg cgagcgtcag gccgggcagc
     8101 gcgatcatgg tgtcgcgccc ccaatcgccg aaccagggat agccggcaat caccgattcc
     8161 ccgtccggca cttccgggag cgggcgcgcg aacacgaagc tgtccgccgc cagcaccagc
     8221 tggtcgatcc attgcggcgc gccgagcagc tccggcgcct gcacctgggc gcggcgcagc
     8281 tgaccgacat cgcgcgcctg ggcgcgccgc agcgcctcgt ccaggtagac cgaagcattc
     8341 gcctcgaggc tcgcgaccac gccgacccat tggccgggat gcaagatgag ctgcgcctcg
     8401 ccgacgcaca gatggcagtc gcgatcgggc aggccgcggt cgcgttccag ggccagatcg
     8461 aagttctcgt accagtcgtg acgcgtggcg atgccgccgc cgcgcacctt gaggacggcc
     8521 gagaaccagt tcgggtgcac gacccgcagc tccgagccgc tgccttcgct cccaccgctg
     8581 ctctcgatga cagggttgaa gtcccagggc cgtgcagtgc cgtggaaatc gcgcgcgttg
     8641 accagcagct tcacacgcag ctcgggcgga cgctggggtg gcgtgctgtc cggcgccagg
     8701 cgccaggcga cgtaagtcgt gctctcgccg tactgcatcc agatgcgctg ctcgacgcgc
     8761 agctcgccgc aggcatagcg ccagaccgga atgcgcccgt cgaggcggaa cgactcgatg
     8821 ttgagccagc cggccggttg cacggcgccg ccgctccagc ggttggtcgc gagcgcccag
     8881 ctgcgctctc cgtcgatgag cgtagcatcc gccttcgcca ccaccagatg gcgccccagc
     8941 ggcggctgca ccggggccac cagcaggccg tgatagtggc gtgtcagcgt tccggcgacg
     9001 gtccccgccg cgaagccacc caggccgttg gacagccacc attcgcggcg ctcggcctgc
     9061 gccaggtcgc cgcagatatc gcggccgaag cggatcagat ccggcagtct ggtttccacg
     9121 tttgcctccc gatggaaagc cgatcagggg ttcttcattg cactgcggcg gcggccctgc
     9181 gcggtgccgc gcgcgaatcg agcaggctcg ccacgcgcag cgcccaggcg tagatggtga
     9241 gggccgggtt gacgcggctc gagcgcggca gcgcgctgcc gtcgaccacg tacagattat
     9301 ccatgccgtg cacgcgcccg accgcatcga ccaccgagcg cgtcggatcg gtgccggcga
     9361 cgagcgtgcc gcaggcgtgc gccgtgccgg agatggggat ggcgcgcgcc acgctcacat
     9421 agccgaggcg cagcagctgc cggcgcaggg tgctcaccag tgcggcatgc tcggcatagg
     9481 cttcgggcag gcgggccggg tcgtagtcga tttgcggccg cggcggcgaa ccggcggcgg
     9541 cgaggatgcg gttgtcggga tgggagccgt cttcggtctg caggaacagc ccgagcgcgc
     9601 gctgcgcgaa aggctccacc agcggccgcg gcgccagcgc aggcgcctgc acccgcagga
     9661 tctcgcaggc cagattgccg cccaatgcct gcaccgtgct gtgcgcgaac cccgtgtggg
     9721 tcagcagcag cgtcttgccc agcacgtcgc gcacccggcg cggcgagaag gcgagcatgg
     9781 cggtgagcac gtggaacttg taattgcggc cgacgttgcg gtaggccggc agcgaggccg
     9841 caaggcccgt ggcctccaga tagcgctgca gcagccgcgg gctgtgcagc gctccggcgg
     9901 cgagcagcac cgtgtcggcg tgatagcgcg tgccgtcctc gcactcggcg ccgcccaccc
     9961 ggatcgcacc gtgcggcgcg ggcagcagcg tccgcaccgc cttgccggtg aggatctcga
    10021 ggttggggcg gctgcgcacg cggtcgagca ggcagcgctc cgcgtcgctc ttcaggccgc
    10081 gcgcggaagc gaacccgtcg aaatggcgca cctcctgggg gtggtcgagg atcccggcgt
    10141 cgagcccgag cttgagggtc tgcgtgctcc agccggcgtc cagccggcgc agcccgccgg
    10201 cgagccggcg cagatcggcc tcgacggtga atgtgcgcac cgccagcagg cgttcggcct
    10261 cctcgtagaa cggcgccagc tcctcgtagg cgatcggcca gggcaggaag ccgcgagcgg
    10321 gatcgggctc gaactcccgc ggtgcgaagc gcagcagcgc cgctccgtac cacttggtct
    10381 tgccgcccag gttgaagtgc tcctcgggcg tggtgacgtt ggcgttgcgg tccagccacg
    10441 gctcgtcggc caggaaggcc ccgcgccgca tcaccgtgtc gacgtcgagc gtactgccgt
    10501 cggcgggcag gcggtgacct ttctcgagca gcaggacgcg cctgccggtc tgggtcaagt
    10561 gataggccgc agccgatcct ccggcgcccg agccgatgac gatgacgtcg tatttcacct
    10621 tgcagctcct ggaaggatct tcagtcgtgt ctttgaggct ccgcggttcg ggcgtacgct
    10681 cacggctcga ccagcagcac cgcgtggccg tcgggatcgc gcaccatcag ccccttgcgc
    10741 cacggcatgc cggacacgct caccggctgg ggtgagatga aactcgcctt ctgcgcctgc
    10801 agcttgtcgg ccagggcggc caggtcgtcc acctccacga tcgttcgcgt ggccaccagg
    10861 tcgttggccc gcaggccgat cggtgcaggc cggccgcccg acggggtgag gtactgcagg
    10921 aactcgagcc ctgggctcgc atcgcgctcg ggcttgagcc cggtgatgcg caccaccgcg
    10981 ccgaatgcgt tgtcgagctg ctcctgggtg gtgccggagt tgaggctgcc gccggccacc
    11041 gcgagtccga ccaggtcgcg ccagaaaccg gtgctgcgct cggtgtcggc caccgtgatc
    11101 gcggagtgat cgatccccag gaacagccgg ctcttgtcct gccacttggc gcgccccttg
    11161 tcgggaggga agtacaacag ctcgagcgga tggccgtccg ggtccttgaa cttgtaggcc
    11221 ttgatgccgg cggcggcccg gttcgaggcc ggaatggtct gcggagcgct ggagatcgac
    11281 tgcacgccga acccgcgcac gtgctcatac gccttctcca tgtcgctcac caccacggcg
    11341 aagtgctgga accacagatc gttggagcgg ctgtcgagcg gaatcggttg cccggcggcg
    11401 gcgacgtact gctccagctc gacgaactcg tcgccgagct gcaggcgcgc catgcgcacg
    11461 cgcgtgccga agatgccggt aagagcgtct tgatcggggc cggcga
//
LOCUS       DASPGE010000219         5568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1581692, whole genome shotgun sequence.
ACCESSION   DASPGE010000219 DASPGE010000000
VERSION     DASPGE010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581692"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VD791_07070"
     CDS             complement(<1..525)
                     /locus_tag="VD791_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835092.1"
                     /note="catalyzes the reversible formation of glyoxylate
                     and succinate from isocitrate; glyoxylate bypass pathway;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HXV07771.1"
                     /translation="MQAREQAVAALKKDWAENPRWAGVKRGYSAEDVVRLRGSLKIEH
                     TLARLGAEKLWRLCLERPFVNSLGALTGNQAMQQVRAGVPAIYLSGWQVAADANDSLS
                     MYPDQSLYAVSSVPTVVKRINNALMRADQIQTMEAVDGKKGGDTDWFAPIVADAESGF
                     GGVLNAFELMKAMID"
     gene            complement(704..1816)
                     /gene="rodA"
                     /locus_tag="VD791_07075"
     CDS             complement(704..1816)
                     /gene="rodA"
                     /locus_tag="VD791_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HXV07772.1"
                     /translation="MEIAGYPLRRLRAQIVEGIDPVLFTLALVLSLLGLAVLYSASYE
                     NAARVANQTGNLALALTAMWAVAKVPPQRLARLAVPAYAVGVVLLLAVAGLGEVVNGA
                     RRWLNLGFMRFQPSEMMKIAMPMMLAWYFHRREGAIRLRDFALAGLLLAVPVALVARQ
                     PDLGTALLVTAAGFYVIFFAGIGWKVLAGLVVLGLSSLIPLWGLLHDYQRRRVLTLLD
                     PSQDPLGAGYHTIQSTIAVGSGGLAGKGWLNGSQTHLEFIPERHTDFIFAVFSEEFGL
                     LGNLALLALYSLLIARGLTIAAGAATLFARLLAGAITLIFFTYAFVNMGMVAGVLPVV
                     GVPLPFISYGGTALLTLYVGIGMLMSIHRHRRLIQT"
     gene            complement(1816..3732)
                     /gene="mrdA"
                     /locus_tag="VD791_07080"
     CDS             complement(1816..3732)
                     /gene="mrdA"
                     /locus_tag="VD791_07080"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXV07773.1"
                     /translation="MRFAELRNYERELQGFQLRLGVAGLAVLVAFGLLLARFVWLQVL
                     QHDAYSALAEENRIAIVPLPPNRGLIVDRNGEILARNYLAYTLEIFPAQVRNLEATIS
                     QLAAYVDIQPRDRARFRKLLQETKNAESLPIRTRLTDEEVARVAANRWRFTGADIRAR
                     LFRQYPHGDLASHVVGYMGRVTTADVESLEATGDAANYRGTDYIGKAGIEASYQQELH
                     GTTGFERVEIDAAGRGVRTLPPVTQPQPGNNVSLTLDIRLQEVAERAFGERRGALVAI
                     EPATGGVLAFVSRPGYDPNLFVDGIDPQSWEALMSSPDRPLNNRAIAGLYPPGSTFKP
                     FMALAALELGKRTPASAIQDPGYFIFGDRRFRDSKPGGHGTVDLYKSIVVSSDVYYYM
                     LANDMGIEAIAGFMGRLGFGARTGIDLDGEMAGVLPSPEWKHARFSQPEQQKWYPGET
                     ISIGIGQGYNSYTPLQLAQALATLANNGAMYRPRLVSHIDNPRTGERQFIEPQLVRQV
                     GLRPEHVAFIKRAMAGVTTEGTGRWAFAGAQYTSGGKTGTAQVIGMKQGEKYEEEKVA
                     ERLRDHSLYIAFAPLENPRIALAVLVENGGFGARAAAPIARTVLDYFLLGKLPAAPRK
                     PAPVREPAGDAQSD"
     gene            complement(3729..4247)
                     /gene="mreD"
                     /locus_tag="VD791_07085"
     CDS             complement(3729..4247)
                     /gene="mreD"
                     /locus_tag="VD791_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HXV07774.1"
                     /translation="MAFDRSRHLLLPVRTGTIVASFVGALLLEFLPWPDVALAPDFVA
                     LTLAFWCVHHPRRVGLATAWALGLLVDAGNGVLLGQHALAYSALAFAAIALSRRILWF
                     DFWGQGLHVLLLLQGAQAIALGVRLAAGDEFPGWSLFAGPAVATLLWPAVVWLLQLPQ
                     RRPTSIDETRPL"
     gene            complement(4237..5154)
                     /gene="mreC"
                     /locus_tag="VD791_07090"
     CDS             complement(4237..5154)
                     /gene="mreC"
                     /locus_tag="VD791_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HXV07775.1"
                     /translation="MEHTPPPFFVRGPAPLVRLAFFASLSLALLVLDARFGYAEGVRS
                     VLALAAYPIQQAARLPVLIVEGATGFFATQSRLREENEALRARLLAATLDAQRYESAA
                     AEASNLRRLIGATERLERKSIPAEVLYAGRDPYARKVIIDRGTQHGVRPGSAVVDDTG
                     VIGQLTRAHAFVSEVTLLTDKDLAIPVQVLRNGLRAIAFGGGASGLLELRYMAANADV
                     ENGDVLVTSGIDGTYPAGLPVASVVRVERDASYTFARVLAQPLAGVERGRYALVLSAG
                     ASLPDYPKDAEERKGAKTRRAKKPVADGV"
     gene            complement(5190..>5568)
                     /locus_tag="VD791_07095"
     CDS             complement(5190..>5568)
                     /locus_tag="VD791_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HXV07776.1"
                     /translation="IGSAFPGSEVKEMEVKGRNLAEGIPRSFTISSNEILEALTEPLN
                     QIVGAVKSALEQTPPELGADIAEKGMVLTGGGALLRDLDRLFMEETGLPVIVADDPLT
                     CVVRGSGKALEKMEHFGPIFTND"
BASE COUNT          877 a         1899 c         1993 g          799 t
ORIGIN      
        1 gtcgatcatc gccttcatca gctcgaaggc gttgagcacg ccgccgaagc ccgactcggc
       61 gtcggcgacg atcggcgcga accagtcggt gtcgccgccc ttcttgccgt ccacggcttc
      121 catcgtctgg atctggtcgg cgcgcatcag cgcgttgttg atgcgcttca ccaccgtcgg
      181 caccgaggac accgcgtaca gcgactggtc gggatacatg gacagcgaat cgttggcgtc
      241 cgccgccact tgccagcccg agaggtagat ggcgggcacg cccgctctca cctgctgcat
      301 ggcctggttg ccggtgagcg cgccgagcga gttgacgaac ggccgctcca ggcacaggcg
      361 ccacagcttc tcggcgccga ggcgcgccag cgtgtgctcg atcttcaggc tgccgcgcag
      421 ccgcacgacg tcctcggcgc tgtagccgcg cttgaccccg gcccagcgcg ggttctcggc
      481 ccaatccttt ttcagtgcgg caacagcctg ttcgcgagcc tgcatgtgcg tgtctccaga
      541 aagtgggttg atctggagtc gaccatagcc aaggttatgc cgcacagcaa tagcgtgatc
      601 gcgggaggac accgtcccgt aaaacaagga tttacaacag cttaagcgat catcatttca
      661 tgatgcgaat cggtatttca gtattctgtt gctgcgcaca aattcaggtc tggatgagcc
      721 ggcgatggcg gtggatgctc atcagcatgc cgatgccgac gtagagcgtg agcagcgcgg
      781 tgccgccgta ggagatgaaa ggcaagggca cgcccacgac cggcaggacg cccgccacca
      841 tgcccatgtt gacgaacgca taggtgaaga agatcagggt gatcgcgccg gcgagcaggc
      901 gcgcgaacag cgtcgccgcc ccggcggcga tggtcaggcc gcgcgcgatc agcagcgagt
      961 acagcgcgag cagcgcgagg ttgccgagca gcccgaactc ctccgagaac acggcgaaga
     1021 tgaaatcggt gtggcgctcc gggatgaact cgaggtgcgt ctgcgagccg ttcagccagc
     1081 ccttgcccgc cagcccgccc gagcctaccg cgatggtcga ctggatcgtg tgatagcccg
     1141 cgccgagcgg atcctgcgag ggatcgagca acgtcagtac gcgccggcgc tggtagtcgt
     1201 gcaacaggcc ccacagcgga atgaggctgg agagccccag cacgaccagc ccggccagca
     1261 ccttccagcc gatgccggcg aagaagatga cgtagaagcc ggcggcggtg accagcagcg
     1321 ccgtgcccag gtccggctgg cgcgccacca gcgccaccgg cacggcgagc agcaggccgg
     1381 cgagcgcgaa atcgcgcagc cggatggcgc cttcccggcg gtggaagtac cacgccagca
     1441 tcatcggcat ggcgatcttc atcatctccg agggctggaa gcgcatgaaa cccaggttca
     1501 gccagcgccg cgcgccgttc acgacttcgc ccagccccgc gacggcgagc agcagcacga
     1561 caccgacggc ataggcgggc acggccaggc gggcgagcct ctgcggcggc accttggcca
     1621 ccgcccacat ggcggtgagc gccagcgcca ggtttcccgt ctggttggcg acgcgcgcgg
     1681 cgttctcgta gctcgcggaa tacagcaccg cgagcccgag caggctgagc acgagcgcca
     1741 gcgtgaacag caccggatcg atgccctcga cgatctgcgc gcgcaggcgc cgcagcggat
     1801 agccggcgat ctccatcagt cgctctgcgc gtcgcctgcc ggctcgcgca ccggcgccgg
     1861 cttgcgcggc gccgcgggca gcttgccgag caggaagtaa tcgagcaccg tgcgcgcgat
     1921 gggtgcggcg gcgcgcgcgc cgaagccgcc gttttccacc agcacggcga gcgcgatcct
     1981 gggattctcc aggggcgcga aggcgatgta cagcgagtgg tcgcgcagcc gctcggcgac
     2041 tttctcctcc tcgtacttct cgccctgctt catgccgatg acctgcgcgg tgccggtctt
     2101 gccgccgctc gtgtactgcg cgccggcgaa ggcccaccgg cccgtgccct cggtggtcac
     2161 gcccgccatc gcccgcttga tgaacgccac gtgctccggg cgcaacccga cctggcgcac
     2221 cagctgcggc tcgatgaact ggcgctcgcc ggtgcgcggg ttgtcgatgt gcgacaccag
     2281 gcgcgggcgg tacatggcgc cgttgttggc cagcgtggcg agcgcctgtg cgagctgcaa
     2341 gggagtgtag gagttgtagc cctggccgat gccgatggag atcgtctcgc cggggtacca
     2401 cttctgctgc tcgggttgcg agaagcgcgc gtgcttccac tcgggcgagg gcagcacgcc
     2461 ggccatctcg ccgtcgaggt cgatgccggt acgcgcgccg aagcccaagc gccccatgaa
     2521 gccggcgatg gcctcgatgc ccatgtcgtt ggccagcatg tagtagtaca cgtcggagga
     2581 cacgacgatc gacttgtaca ggtccacggt gccgtgcccg ccgggcttgg agtcgcgaaa
     2641 gcgccggtcg ccgaagatga agtagccggg atcctggatg gcgctcgccg gcgtgcgctt
     2701 gccgagctcc agcgcggcga gcgccatgaa cggcttgaag gtggaccccg gcgggtacag
     2761 cccggcgatc gcgcggttgt tgagcggccg gtcgggcgag ctcatgagcg cctcccagct
     2821 ctgcgggtcg atgccgtcga cgaacaggtt cgggtcgtag cccggccggg agacgaaggc
     2881 gagcacgccg ccggtggccg gctcgatggc cacgagcgcg ccgcgccgct cgccgaacgc
     2941 gcgctcggcg acctcctgca gccggatgtc gagcgtcagg ctgacgttat tcccgggctg
     3001 gggctgggtc acgggcggca gcgtgcgcac gccgcgaccg gcggcgtcga tctccacgcg
     3061 ctcgaagccg gtcgtgccgt gcagctcctg ctggtagctt gcctcgatgc cggccttgcc
     3121 gatgtagtcc gtcccgcggt agttggccgc gtcgccggtc gcctcgaggc tctccacgtc
     3181 ggcggtggtg acgcgcccca tatagccgac gacgtgcgag gcgaggtcgc cgtgcgggta
     3241 ctggcggaac agacgcgcgc ggatgtcggc gccggtgaag cgccagcggt tggcggcaac
     3301 gcgcgccacc tcctcgtcgg tgagccgggt gcggatgggc aggctctcgg cgttcttcgt
     3361 ttcctgcagg agcttgcgga agcgcgcgcg gtcgcgcggc tggatgtcga cgtaggccgc
     3421 gagctgcgag atcgtcgcct cgagattgcg cacctgcgcg gggaagatct ccagcgtgta
     3481 ggcgaggtaa ttgcgcgcca ggatctcgcc gttgcgatcg acgatcaggc cgcggttggg
     3541 cggcagcggc acgatggcga tgcggttctc ctccgccagc gcgctgtagg cgtcgtgctg
     3601 cagcacctgc agccagacga agcgcgccag cagcagcccg aaggcgacca gcaccgcgag
     3661 gccggccacg cccaggcgca gctggaagcc ctgcagctcg cgctcgtagt tgcgcagctc
     3721 ggcgaacctc acagcggccg cgtctcgtcg atggaggtgg gccggcgctg cggcagctgc
     3781 agcagccaga cgaccgcggg ccagagcagc gtcgccacgg ccgggccggc gaacagcgac
     3841 cagcccggga attcgtcccc ggccgccagg cgcacgccga gcgcgatggc ctgggcgcct
     3901 tgcagcagca ggagaacgtg cagcccctgg ccccagaagt cgaaccacag gatgcggcgc
     3961 gacagcgcga tggccgcgaa ggcgagcgcc gagtaggcga gcgcgtgctg cccgagcagc
     4021 acgccgttgc cggcgtccac cagcagcccg agcgcccagg cggtggccag tccgacccgg
     4081 cgcggatggt gcacgcacca gaaggcgagc gtcagcgcga cgaagtccgg cgcgagcgcc
     4141 acgtccggcc agggcaggaa ttccagcagc agggcgccga cgaacgaagc gacgatggtg
     4201 cccgtacgca ccggcagcag caggtggcgg gagcggtcaa acgccatcgg ccaccggctt
     4261 cttcgcgcgc cgggtcttgg cgcccttgcg ttcctcggcg tccttcgggt agtcgggcag
     4321 gctcgcgccc gccgacagca ccagcgcgta gcggccgcgc tcgacgccgg cgaggggctg
     4381 cgccagcacg cgcgcgaagg tgtaggacgc gtcgcgctcc acgcgcacca ccgaggcgac
     4441 cggcagtccg gcggggtagg tgccgtcgat gcccgaggtg accagcacgt cgccgttttc
     4501 cacgtcggcg ttggcggcca tgtagcgcag ctcgagcagt cccgaggcgc cgccgccgaa
     4561 cgcgatggcg cgcagcccgt tcctcagcac ctgcacggga atcgccaggt ccttgtcggt
     4621 gagcagggtc acctcggaca cgaaggcgtg cgcgcgcgtg agctggccga tgaccccggt
     4681 gtcgtccacc accgcgctgc ccggccgcac gccgtgctgc gtgccacggt cgatgatcac
     4741 cttgcgggca tacgggtcgc gcccggcgta cagcacttcc gcggggatgg acttgcgctc
     4801 caggcgctcg gtcgcgccga tcagccgccg caggttgctc gcctcggcgg cggccgactc
     4861 gtagcgctgc gcatcgagcg tcgcggcgag caggcgcgcg cgcagcgctt cgttctcctc
     4921 gcgcagccgg gactgcgtgg cgaagaaccc ggtggcccct tcgacgatca gcaccggcag
     4981 gcgcgcggcc tgctggatcg ggtaggcggc gagcgccagc accgagcgca cgccctcggc
     5041 gtagccgaag cgcgcatcga gcaccagcag ggcgagagaa agcgaggcga agaacgcgag
     5101 ccggacgagg ggagcgggtc cgcggacgaa gaagggcggg ggagtgtgct ccacgtcagc
     5161 gaagggaggg cgggcccttc acccctcggt cagtcgttgg tgaagatcgg gccgaagtgc
     5221 tccatcttct cgagcgcctt gcccgagccg cgcaccacgc aggtgagcgg gtcgtcggcg
     5281 acgatcaccg gcaggcccgt ctcttccatg aacaggcgat cgaggtccct gagcagcgcg
     5341 ccgccgccgg tgagcaccat gcccttctcg gcgatgtcgg cgcccagctc gggcggggtc
     5401 tgctcgagcg cggacttgac cgcgccgacg atctggttga gcggctcggt gagcgcttcc
     5461 aggatctcgt tcgagctgat ggtgaagctg cgcgggatgc cttcggcgag gttgcgtccc
     5521 ttcacctcca tctccttcac ttccgacccg gggaacgcgg agccgatc
//
LOCUS       DASPGE010000220         6374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1463713, whole genome shotgun sequence.
ACCESSION   DASPGE010000220 DASPGE010000000
VERSION     DASPGE010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463713"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VD791_07100"
     CDS             <1..359
                     /locus_tag="VD791_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07777.1"
                     /translation="MQDAAVTAPSNENTPANVFGTYMQLASDDVWGSSLNDAQRINIK
                     TGNMISVEILERVDSKTDDGRPGSGRFQFSTYAAVGAAPATGGSEVACTDLDSVDAVW
                     DVRSGQGNCGAATLLR"
     gene            complement(406..2874)
                     /gene="lon"
                     /locus_tag="VD791_07105"
     CDS             complement(406..2874)
                     /gene="lon"
                     /locus_tag="VD791_07105"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HXV07778.1"
                     /translation="MRQALEMIEHAHRMDGNVSGPGQEAPRPASSQGAALVLPDGAIP
                     IVPVRNLVLFPGMILPLTVGREGSIAAAQQAVRSERPIGLLLQKDPKQDNPGGDDLHA
                     FGTVAQVLRYVTTQDGGHHVICQGQERFRVREFLSGYPFLVARVERFADGAAASQDIE
                     ARFLQLKRRAGEVLELLPQTPQELVTAVQNFTSPGGLADMVAGFMDVTPEEKQQLLET
                     LNVQTRLDKVLDLLARRIEVLKLSKEIDERTKETVDKRNREFLLREQLRTIRQELGEG
                     EEGGGDLEELEKAITEAGMPEEVEAHARKELKRVARIPEASAEYSMARTYLDWLIELP
                     WKTSAQSEIDLAEARRILDQDHHGLDKVKKRILEFLAVRKLNPQGKSPILCFVGPPGV
                     GKTSLGQSIARALGRKFTRVSLGGVHDESEIRGHRRTYVGALPGNVIQALKKTGSREC
                     VMMLDEIDKIGLGGFHGDPASALLEVLDPEQNHSFRDNYLGVPFDLSRVTFIATANVL
                     ETSPGPPRDRMEIIHLPGYTQEEKLQIATKYLVKRQLEQNGLPADKFEIAEAAIDAII
                     RDYTREAGVRNLEREIGAVCRSAAVKIAAGEAQRVRVDAGDLHEILGAARFEAEVAAR
                     ATVPGVATGLAWTPVGGDILFIEASRTPGNGKLILTGQLGDVMKESAQAALTLVKSRT
                     ADLGVDPAAFDRSDVHVHVPAGAIPKDGPSAGVAMFMALASLLSNRVVRHDTAMTGEI
                     SLRGLVLPVGGIKEKVLAAMRAGITTVMLPKRNQKDLQDVPQEAREKLRFVWLETVDG
                     AMAEALRPALRPESALRQEPALAD"
     gene            complement(3028..3456)
                     /locus_tag="VD791_07110"
     CDS             complement(3028..3456)
                     /locus_tag="VD791_07110"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXV07779.1"
                     /translation="MDKAHDADKPALAVLHPAGWARPRGYSNGIAATGKQVFVAGQIG
                     WDEQCRLVSSDFAEQARQALRNVVAVLKEAGAGPQHLARMTWYLTDKGEYLAAAKAIG
                     QAYREIVGPHYPAMTAIQVSALIEDGAKVEIEATAVVPTD"
     gene            complement(3472..4182)
                     /locus_tag="VD791_07115"
     CDS             complement(3472..4182)
                     /locus_tag="VD791_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV07780.1"
                     /translation="MNALLEVSGLEAGYGQSQVLFGIDLRVQEGEVAALLGRNGMGKT
                     TTLKALLGLLPARRGSVRFRGERIDGRSTDRIARAGMALVPEGRQIFPNLSVEENLLA
                     FAGNRRGAAEPWTAQRVYGLFPRLHERRRSLGNQLSGGEQQMLAIGRALMTNPHLLIL
                     DEATEGLAPLVREEIWRCLHALSRQGQTILVIDKYVERLIALAHQHTIVERGRVVWQG
                     SSQALDADHGIWHRYLGI"
     gene            complement(4179..4943)
                     /locus_tag="VD791_07120"
     CDS             complement(4179..4943)
                     /locus_tag="VD791_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV07781.1"
                     /translation="MVEPLLALRGLTKRFGGLTATDGVTLDIAPGEIHALIGPNGAGK
                     TTLIHQISGALPPDHGSVTFGGREITRLAMHERVAAGLVRSFQVTNIFQRFSVLDNVA
                     LAVQAGSGSSMGFWRPVAREAALFGEARELLARIDLADKAASTAATLAHGEQRQLEVG
                     LALATRPKLLLLDEPLAGMGPEESERMVQLVAGLARTCTILLVEHDMQAVFRLATRIS
                     VLVSGRIIASGAPDDVRRDSEVRRAYLGDEVPSAAA"
     gene            complement(4936..5811)
                     /locus_tag="VD791_07125"
     CDS             complement(4936..5811)
                     /locus_tag="VD791_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV07782.1"
                     /translation="MQAIDQPFYIVLATRILIFALAASSLNLLIGFGGMVSFGHAAFF
                     GTGAYTVGILMVHGVDSAWIAWPAAVLVSALAALLIGAISLHTRGVYFIMITLAFAQM
                     IYYVFVSLKAYGGDDGLTLAGRSQVGFGVDLASDIVFYYVVLAIVATLLVLLGRLTNA
                     RFGRALQAIRENETRAQAIGFPAYRYKLACFVLGGAMAGLAGVLIVNQNELASPNLLH
                     WMQSGMLMIMVILGGVGFLYGGVLGALVLLLLEETLSEYTIHWQFGVGLVLLAVVLFA
                     PNGLLSLARRRTAGG"
     gene            complement(5896..>6374)
                     /locus_tag="VD791_07130"
     CDS             complement(5896..>6374)
                     /locus_tag="VD791_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV07783.1"
                     /translation="GVGLVVAVALYLLVTHTRIGMLVRAGASNREMALTMGVNVKRLF
                     TAVFAAGAALSALAGVLLGPLLAVQVGMGESILILAFVVIVIGGIGSIRGALVGALLV
                     GFVDTLGRTLLPMSLRSVLPPMVASNLGPALASILIYVLMAAVLFWRPQGLFPARG"
BASE COUNT          999 a         2286 c         2117 g          972 t
ORIGIN      
        1 cgatgcagga cgcggcggtg acggcgccgt cgaacgagaa caccccggcc aatgtgttcg
       61 gcacctacat gcagctggcg tccgacgacg tctggggcag ctcgctcaac gacgcgcaac
      121 gcatcaacat caagaccggc aatatgatct cggtggaaat tctagagcgg gtggacagca
      181 agaccgacga cggcaggccg ggatcgggcc gcttccagtt ttccacctac gcggccgtcg
      241 gggccgctcc ggcgacaggc ggctcagagg tcgcgtgcac cgacctcgac tccgtcgatg
      301 ccgtctggga cgtgcgcagc ggacaaggaa actgcggcgc ggcgactctg ctgcgctagc
      361 ccgatagggg atttgcgcta gaccggccgc cggcagccgg ccgactcaat ccgccagcgc
      421 cggctcctgg cgcagcgccg actccgggcg cagcgccggg cgcagcgcct cggccatcgc
      481 gccgtccacg gtctccagcc agacgaagcg cagcttttcg cgcgcctcct gcggaacgtc
      541 ctgcagatcc ttctggttgc gcttaggcag catcacggtg gtgatgcccg cgcgcatggc
      601 ggccagcact ttttctttga taccgccgac cggcagcacc aggccgcgca ggctgatctc
      661 tccggtcata gcggtgtcgt gccgcaccac ccgattcgac agcagcgagg cgagcgccat
      721 gaacatggcc acgccggcgc tgggcccatc cttggggatg gcgcccgccg gaacgtgcac
      781 gtgcacgtcg gacctgtcga acgcggccgg gtcgacgccc aagtcagccg tgcgcgattt
      841 caccagggtc agcgccgcct gcgcgctttc cttcatcacg tcgccgagct gcccggtcag
      901 gatcagcttg ccgttgcccg gcgtgcggct cgcctcgatg aacaggatat cgccgcccac
      961 cggcgtccag gccagcccgg tggccacacc cggcaccgtg gcgcgcgccg ccacttccgc
     1021 ttcgaagcgc gccgcgccca ggatctcgtg cagatcgccg gcgtccacgc gcacgcgctg
     1081 cgcctcgccc gccgcgatct tgaccgcggc gctgcggcat accgcgccga tctcgcgctc
     1141 gagattgcgc acgccggcct cgcgcgtgta gtcgcgaatg atggcgtcga tcgcggcctc
     1201 ggcgatctcg aacttgtccg ccggcaggcc gttctgctcg agttgacgct tgaccaggta
     1261 cttggttgcg atctgcagct tctcctcctg cgtgtagccg ggcaggtgga tgatctccat
     1321 gcggtcgcgc ggcggcccgg gactggtctc gagcacgttg gcggtggcga tgaaggtcac
     1381 ccgcgacagg tcgaagggca cgcccaggta gttgtcgcgg aagctgtggt tctgctccgg
     1441 atcgagcacc tcgagcagcg ccgaagccgg gtcgccgtgg aatccgccga ggccgatctt
     1501 gtcgatctcg tccagcatca tcacgcattc gcggctgccg gtcttcttca gcgcctggat
     1561 cacgttgccc ggcagcgcgc cgacgtaggt gcggcggtgc ccgcggatct cggattcgtc
     1621 gtgcacgccg cccagcgaga cgcgcgtgaa cttgcgccct aacgcgcgcg cgatgctctg
     1681 gccgagcgaa gtcttgccca cgccgggcgg accgacgaag cacaggatcg gcgacttgcc
     1741 ctgcgggttg agcttgcgca cggcgaggaa ctcgaggatg cgcttcttca ccttgtccag
     1801 gccgtggtga tcctggtcca ggatgcggcg cgcctcggcg agatcgatct cactctgcgc
     1861 ggaggtcttc cagggcagct cgatcagcca gtcgaggtag gtgcgcgcca tcgagtactc
     1921 ggcggaggcc tccggaatgc gcgccacgcg cttcagctcc ttgcgcgcgt gcgcctccac
     1981 ctcctccggc atgccggctt cggtgatggc cttctccagc tcctcgagat cgccgccgcc
     2041 ctcctcgccc tcgcccagct cctggcggat ggtcctgagc tgctcgcgca acaggaactc
     2101 acggttgcgc ttgtccacgg tctccttggt gcgctcgtcg atctccttgg agagcttcag
     2161 cacctcgatg cggcgggcga gcagatccag caccttgtcc aggcgcgtct gcacgttcag
     2221 cgtttccagc agctgctgct tctcttccgg ggtaacgtcc atgaagccgg ccaccatgtc
     2281 ggccagcccg cccggggagg tgaagttctg caccgcggtc accagctctt gcggcgtctg
     2341 cggcaacagt tcgagcacct cgccggcgcg ccgcttcagc tgcaggaagc gcgcctcgat
     2401 gtcctgactg gccgcggcgc cgtcggcgaa acgctccacc cgcgccacca ggaaaggata
     2461 gccggacagg aactcgcgca cccggaagcg ctcctgcccc tggcagatga cgtgatggcc
     2521 gccgtcctgc gtggtcacgt agcgcagaac ctgcgcgacg gtgccgaatg catgcaagtc
     2581 gtcgccgccc gggttgtcct gcttcgggtc cttctgcagc agcaggccga tcggccgttc
     2641 gctgcgcacc gcctgctggg cggcggcgat ggagccttcg cggccgacgg tcagcggcag
     2701 gatcatgccc gggaacagca cgagattgcg caccggcacg atcgggatcg ctccgtccgg
     2761 cagcaccagc gccgctccct ggctcgaggc cgggcgcggc gcctcctggc cggggcccga
     2821 aacgttgccg tccatcctgt gtgcgtgctc gatcatctcg agggcctgtc gcatgcggtt
     2881 gcttccgagg gaaatctccc aatgcccggc gcgcgacccg cgccgctgca gggcatatca
     2941 gggttgagga agggatttca agccaagcgc tgcctctccc ccttcgaccc tcaccccacc
     3001 tctcccgcag gcgggagagc gacctcttca gtctgttgga acgaccgccg tcgcctcgat
     3061 ctccaccttg gcgccgtcct cgatcagcgc cgacacctgg atggcggtca tggccgggta
     3121 atgggggccg acgatctccc ggtacgcctg gccgatggcc ttggccgcgg ccaagtactc
     3181 gcctttgtcg gtcaggtacc aggtcatgcg tgcgaggtgc tgcgggccgg cgcccgcttc
     3241 cttcagcacc gccaccacgt tgcgcaacgc ctgccgcgcc tgctcggcga aatcgctgct
     3301 caccaggcgg cactgctcgt cccagccgat ctggccggcg acgaacacct gcttcccggt
     3361 cgcggcgatt ccgttcgagt agccgcgcgg gcgcgcccag ccggcaggat gcagcacggc
     3421 gagggcgggt ttatctgcgt cgtgtgcttt gtccatggtc ttcgtcctcc atcaaattcc
     3481 tagataccga tgccagatgc cgtgatcggc atcgagcgcc tgcgagctgc cctgccacac
     3541 gacgcgcccg cgctccacga tcgtgtgctg gtgcgcgagc gcgatcagcc gctcgacgta
     3601 cttgtcgatc accaggatgg tctgtccctg gcggctcagc gcgtgcaggc agcgccagat
     3661 ctcctcgcgc accagcggcg ccagcccctc ggtggcctcg tccaggatca gcagatgggg
     3721 gttggtcatg agcgcccgcc cgatcgcgag catctgctgc tcaccgcccg agagctggtt
     3781 gccaaggctg cggcggcgct cgtgcagccg gggaaacagg ccgtagacgc gctgtgcggt
     3841 ccatggctcg gctgcgccgc gccggttgcc ggcgaatgcc aggaggttct cctcgacgct
     3901 caggttggga aagatctggc ggccctcggg caccagggcc atccccgcgc gcgcgatgcg
     3961 gtcggtggac cgcccgtcga tgcgctcgcc gcggaagcgc acgctgccgc gccgcgccgg
     4021 cagcagcccg agcagggcct tgagcgtggt ggtcttgccc atgccgttgc gcccgagcag
     4081 cgcggcgacc tcgccctcct gcacccgcag gtcgatgccg aacaggacct ggctctgtcc
     4141 gtagccggct tcgaggccgg agacctccag cagcgcgttc atgctgcggc gctcggcact
     4201 tcgtcgccga ggtatgcccg gcgcacctcg ctatcccggc gcacgtcgtc gggcgcgccg
     4261 ctggcgatga tgcggccgga cacgagcacc gagatccgcg tcgccagccg gaacaccgcc
     4321 tgcatgtcgt gctccaccag taggatggtg caggtacggg cgaggccggc gacgagctgc
     4381 accatgcgct ccgactcctc gggtcccatc cccgccagcg gctcgtcgag cagcagcagc
     4441 ttcggacgcg tcgccagcgc gaggccgact tcgagctggc gctgctcgcc atgcgccagc
     4501 gtggccgccg tgctggccgc cttgtcggcg aggtcgatgc gggcgagcag ctcgcgcgcc
     4561 tccccgaaca gcgcggcctc gcgcgccacc ggccgccaga accccatgct cgaaccgctg
     4621 ccggcctgca ccgccagcgc gacgttgtcc agcacgctga agcgctggaa gatgttggtg
     4681 acctggaacg agcgcaccag gcctgcggcc acgcgctcgt gcatcgccag ccgcgtgatc
     4741 tcgcgtccgc cgaacgtgac gctgccgtga tcgggcggca gcgccccgga gatctgatgg
     4801 atcagcgtgg tcttgcccgc gccgttgggg ccgatcaggg cgtggatctc tcccggggcg
     4861 atgtccagcg tcaccccatc ggtggcggtg aggccgccga agcgcttggt gaggccacgc
     4921 aacgccagca gcggctcaac cacccgcagt cctccgccgc gccaggctga gcagcccgtt
     4981 gggcgcgaac agcaccaccg ccagcagaac caggcccacg ccgaactgcc agtggatggt
     5041 gtactccgac agcgtctctt cgagcagcag cagcaccagc gcacccagca cgccgccgta
     5101 gaggaaaccg acgccgccga ggatcaccat gatcatcagc atccccgact gcatccagtg
     5161 caggagattg gggctggcca gttcgttctg gttgacgatc agcactccgg ccaggcccgc
     5221 catcgcgccg ccgaggacga agcaggcgag cttgtagcgg tacgccggga aaccgatcgc
     5281 ttgcgcgcgc gtctcgtttt cgcggatcgc ctgcagcgcg cgcccgaagc gcgcattggt
     5341 gagccgcccg agcagcacca gcagcgttgc gacgatcgcc agcacaacgt agtagaagac
     5401 gatatcggac gcgagatcga cgccgaagcc gacctgcgag cggccggcca gcgtcagccc
     5461 gtcgtcgccg ccataggcct tcagcgacac gaacacgtag tagatcatct gcgcgaaggc
     5521 gagcgtgatc atgatgaagt acacgccgcg ggtgtgcagg ctgatggcgc cgatcagcag
     5581 cgccgccagc gccgacacca gcaccgccgc gggccaggcg atccacgctg aatccacgcc
     5641 gtgaaccatc aagatgccga cggtgtaggc gccggtgccg aagaaggcgg catggccgaa
     5701 gctcaccatc ccgccgaagc cgatcagcag attcaggctg ctcgcggcca gcgcgaagat
     5761 caggatgcgg gtggccagca cgatgtagaa aggctggtcg atcgcctgca tcaccagcgg
     5821 gaaggcggcg agcagggcca gcgcgggcag cgcgacccgc ggcagcagcg aatgatgcag
     5881 ggccgtcacg gcgcgtcaac cgcgagcggg aaacaggccc tgcggccgcc agaacagcac
     5941 cgcggccatc agcacgtaga tcaggatcga ggccagcgcc gggcccaggt tggaggccac
     6001 catcggcggc agcacgctgc gcaggctcat gggcagcagg gtgcgcccca gcgtgtccac
     6061 gaagcccacc agcagcgcgc ccaccagcgc gccgcggatg gagccgatcc cgccgatcac
     6121 gatcaccacg aaagcgagga tcaggatgct ctcgcccatg cccacctgca ccgccagcag
     6181 cggaccgagc agcacaccgg cgagtgcgct cagcgctgcg cccgctgcga acacggccgt
     6241 gaacagccgc ttgacgttca cgcccatggt cagcgccatc tcgcggttcg aggcgccggc
     6301 ccgcaccagc atgccgatgc gggtgtgcgt taccagcaga tacagcgcca ccgcgacgac
     6361 caggcccacg ccga
//
LOCUS       DASPGE010000221         6644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_411053, whole genome shotgun sequence.
ACCESSION   DASPGE010000221 DASPGE010000000
VERSION     DASPGE010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411053"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1619
                     /locus_tag="VD791_07135"
     CDS             102..1619
                     /locus_tag="VD791_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXV07784.1"
                     /translation="MASVKRPTLDQMKEIVASLQMSMSEREIGEYLEVMEGTLQAYDR
                     LTQLPDNLPPVRYPRTPGYRPAASENPLNAWAVKSEVRGAPYGPLSGKRVVLKDNVCL
                     AGVPMMNGASTLEGYVPDVDATVVTRILDAGGTIVGKAHCEYFCLSGGSHTSAMGPVH
                     NPFKYGYSAGGSSSGSGALVGSGEVDMAIGGDQGGSIRMPSCWSGCYGMKATHGLVPY
                     TGVMPIEATIDHTGPMSATVADNALLLEVIAGADGLDPRQYNVRVDKYTTALGRGVAG
                     LRIGVLNEGFARPESEADVDQKVREAAERLRALGATLENVSIPMHLDGPAIWTPIALE
                     GLQAQMMAGNGMGFNWKGLYTTSLLDAHANWRARANELSRTLKISMLIGEYFIRHYRG
                     HFYAKAQNLARALTKAYDDVLARYDLLLMPTLPMKATPLPPQNAPLALWCQRGFEMLG
                     NTCAFDVTGHPAMSVPCGMSQGLPIGMMLVARHYNESTIYRAAHAFEQLGDWRNM"
     gene            1725..3449
                     /locus_tag="VD791_07140"
     CDS             1725..3449
                     /locus_tag="VD791_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXV07785.1"
                     /translation="MRSGDERLRPQGGRSYVAGPTDDPLRFVTVPALLDEAVAAHGAR
                     DAVVFAATGTRWSWYDLQRKADEVAAGLLALGIKRGDRVGIWAPNCEEWVGLQFGTAR
                     IGAILVNISPAYRESELEYALNKVHCRVLVMARALKSSDYLAMLRALAPEIERATPDR
                     RLRCERLPRLRHVILLGEGPVPAGAMGFAAFRKLAGPAQRARLSSVAAALDPDDAINI
                     QFTSGTTGVAKGATLSHYNIVNNARYTAQAMRLTERDRACIPVPLYHCFGMVLGVLAC
                     VAAGATMVFPAPTFDAESTLRAVAAQRCTALHGVPSMFIAMLEHPALRGLDVSSLRTG
                     IMAGAPCPVDTMRRVVAELHMREVTIAYGMTETSPVSFQSSVDDPLERRVTTVGRVQP
                     HAEVKIVDEQGRAVAVGQRGELCTRGYLVMRGYWDDDQTTAQAIDPAGWMHSGDLAVI
                     DADGYCNIVGRIKDMLIRGGENVYPAEVEEFLARHPKVQAVQVFGVPDPRLGEDVCAW
                     IVLKPGVIATDEEIRNWCQGRVAHYKVPRHIRFVPAFPATATGKPQKFAMREQMMREL
                     GVRIAASA"
     gene            3514..4551
                     /locus_tag="VD791_07145"
     CDS             3514..4551
                     /locus_tag="VD791_07145"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="App1 family protein"
                     /protein_id="HXV07786.1"
                     /translation="MWLAAGCAARAQGLRRDEQVLLFPSTARSLPEGGGQARIEAWVH
                     ELERRPGATALLARYVGLDLSELPEAERARFTARTQLFRVDSERGRVLQIRFDGEGLL
                     SLPASGADGRVSAVVSNDGTAHASDGPRWLEYAVLMPEEDSRSFAGRALWLPQAGLSI
                     VSDIDDTIKHTQVRDRREMLLNTFARDFEAVPGMPAWLQRCAAAEPAASFHYVSGAPH
                     QLYPPLAEFLHAHAFPPGTVHLRSVDLAREIFAAGGGTRTHKLEVIGHLLDDFPQRRF
                     VLVGDSGEQDPEIYGELARTFPGRIAAILIRDVTGEPADAERYAQALRDVPPALWSLF
                     TEPDRLPAHEF"
     gene            complement(4573..6105)
                     /locus_tag="VD791_07150"
     CDS             complement(4573..6105)
                     /locus_tag="VD791_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXV07787.1"
                     /translation="MNLSLLLHAAATCRPGAPAVTQGSLSWRYGELSRRVAGTAAYLR
                     GRLSAQPGERIGIAMHNSPGTLQLMYGCWHAGLCAVPINAKLHPREFAFILEHSGVRA
                     CFASPSLYPGIRREWTADRASLLEADAALIERLAQAPPMPPHQGQPDDPAWLFYTSGT
                     TGRPKGATLTQRNLLFATHAYYADIDPLDERDTVVHAAPLSHGSGLYSLPHIAKGSHN
                     VITASESFTPGDLFDAIERYLRVSFFAAPTMLMRLVNDGAAAQAQLANLKTVIYGGGP
                     MHVPDLVRALALFGPRLYQLYGQGESPMTITGLSQRDHERALAAHDHEALASAGGPRT
                     GVAVRIVDEADRDLPDGEVGEIVTRSDCVMQGYWRDPAATEAALRGGWLRTGDVGCRD
                     GRGWFTLKDRSKDMIISGGSNIYPREIEDVLLRHARVAQASVVGAPQADWGEEVVAFV
                     VPAAGAAVTAEELDRLCLEHIARYKRPRRYVFVEDLPKNNYGKVLKTALRARLRGEAA
                     DS"
     gene            complement(6110..>6644)
                     /locus_tag="VD791_07155"
     CDS             complement(6110..>6644)
                     /locus_tag="VD791_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV07788.1"
                     /translation="FLPLGTEDYEPTVAQLAASGAQAVLLSLIGQDAIDFNRVFAARR
                     LGGRIRRLSCAIEENELLALDADCTEELYVAGAYFAGLDTDANLAFQARYRALLGLRA
                     PTLNALGQSTYEGVHFLGALLALGGRQPARNEPLPFVSARGAVWRGNDSLTCPVYLAR
                     ADGHALIVTRTLSAGAN"
BASE COUNT          985 a         2318 c         2375 g          966 t
ORIGIN      
        1 ctgcaacgct ttcaccggcg cccgcgcccg cgcgcaccga gtctttcgct gcaccgccac
       61 cgcccgcggc ggcaggcgtc accactacaa cgggtaccac catggcaagc gtcaagcgcc
      121 cgaccctcga ccagatgaag gagatcgtcg cctccctgca gatgagcatg tcggagcgcg
      181 agatcggcga atacctggag gtgatggaag gcaccctgca ggcgtacgac cgcctcacgc
      241 agctgcccga caatctgccg ccggtgcgct atccgcgcac gccgggctac cggccggcag
      301 cgtcggagaa cccgctcaac gcctgggcgg tgaagagcga ggtgcgcggc gcgccgtacg
      361 ggccgctctc cggcaagcgc gtggtcctga aggacaacgt gtgccttgcc ggggtgccga
      421 tgatgaacgg ggcctcgacg ctggagggct acgtgcccga cgtggatgcc acggtggtga
      481 cgcgcatcct ggatgcgggg ggcaccatcg tcggcaaggc gcactgcgag tacttctgcc
      541 tgtccggcgg cagccatacc tcggccatgg ggccggtgca caaccccttc aagtacggct
      601 actccgcggg cggctcctcc tcgggctcgg gcgccctggt gggctccggc gaagtggaca
      661 tggcgatcgg cggcgaccag ggcggctcga tccgcatgcc ctcctgctgg tcgggctgct
      721 acggcatgaa ggccactcat ggactggtgc cctataccgg cgtcatgccg atcgaggcga
      781 ccatcgacca caccggcccg atgagcgcca ccgtcgcgga caacgcgctg ctgctggaag
      841 tgatcgccgg tgccgacggc ctcgacccgc gccagtacaa cgtgcgcgtg gacaagtaca
      901 ccaccgccct gggccgtggc gtggcgggcc tgcgcatcgg cgtgctgaac gaggggttcg
      961 cccggccgga gagcgaagcc gacgtcgacc agaaggtgcg cgaggccgcc gagcggctgc
     1021 gcgcgctggg cgcgacgctg gagaacgtgt cgatcccgat gcatctcgac ggcccggcga
     1081 tctggacccc gatcgcgctg gaaggcctgc aggcgcagat gatggccggc aacggcatgg
     1141 gcttcaactg gaaggggctg tacaccacca gtctgctcga cgcccacgcc aactggcgcg
     1201 cgcgcgccaa cgagctgtcg cgcacgctca agatctcgat gctgatcggc gagtacttca
     1261 tccgccatta ccgcggccac ttctacgcca aggcgcagaa cctggcgcgc gccctgacca
     1321 aggcctacga cgacgtgctt gcgcgctacg acctgctgtt gatgccgacg ctgccgatga
     1381 aggccacgcc gctgccgccg cagaacgctc cgctcgcgct gtggtgccag cgcggtttcg
     1441 agatgctggg caacacctgc gccttcgacg tgaccggaca cccggccatg agcgtgccgt
     1501 gcggcatgag ccagggcctg ccgatcggga tgatgctggt ggcgcggcac tacaacgagt
     1561 ccaccatcta ccgcgcggcg cacgccttcg agcagctcgg cgactggcgc aacatgtgat
     1621 tcgagcaccg gggagccctt gccaacctcg cagccgaaac gaaagccccc ttggggcggg
     1681 ggtgaggggt cgtcgtgagg cccaaccctt tcacttccga cttcctgcgc agcggcgacg
     1741 agcgcttgcg cccgcagggc ggccgttcgt acgtggcggg cccgaccgac gacccgctgc
     1801 gcttcgtcac cgtgccggcg ctgctggacg aagcggtggc ggcgcacggc gcgcgcgacg
     1861 ccgtggtgtt cgccgccacc ggcacgcgct ggtcgtggta cgacctgcag cgcaaggccg
     1921 acgaggtggc ggccgggctg ctcgcgctcg gcatcaagcg cggcgatcgg gtcggcatct
     1981 gggcgcccaa ttgcgaggaa tgggtgggat tgcagttcgg caccgcgcgc atcggcgcga
     2041 tcctggtgaa catcagtccc gcctatcgcg agtcggagct ggaatatgcg ctgaacaaag
     2101 tgcactgccg agtgctggtg atggcgcgcg cgctcaagtc cagcgactat ctcgccatgc
     2161 tgcgcgcact ggcccccgag atcgagcgcg ccacaccgga ccggcggctg cgctgcgagc
     2221 gcctaccgcg cctgcggcac gtgatcctgc tcggagaagg cccggtgccc gccggcgcga
     2281 tgggcttcgc tgcgttcagg aagctcgccg ggcctgcgca gcgcgcgcgg ctatcctcgg
     2341 tggcggcggc gctggatccg gacgacgcga tcaacatcca gttcaccagc ggtaccaccg
     2401 gcgtggccaa aggcgccacc ctgagccact acaacatcgt caacaacgcg cgctacaccg
     2461 cgcaggcgat gcggctcacc gaacgcgacc gggcgtgcat tccggtgccg ctgtaccact
     2521 gcttcggcat ggtgctgggc gtgctggcgt gcgtggccgc gggcgccacc atggtgttcc
     2581 cggccccgac cttcgatgcc gagtcgacgc tgcgcgcggt ggccgcgcag cgctgcaccg
     2641 cgctgcacgg cgtgccgtcg atgttcatcg ccatgctcga gcacccggcg ctgcgcgggc
     2701 tggacgtgtc cagcctgcgc accggcatca tggccggtgc gccctgcccg gtggacacca
     2761 tgcgccgggt ggtggccgag ctgcacatgc gtgaggtcac catcgcctac ggcatgaccg
     2821 agaccagccc ggtgtcgttc cagtcgagcg tggacgaccc gctcgagcgg cgcgtcacca
     2881 ccgtgggccg ggtgcaaccg cacgcggagg tcaagatcgt ggacgagcag gggcgcgccg
     2941 tcgcggtcgg ccagcgcggc gagctgtgca cgcgcggcta cctggtgatg cgcggttact
     3001 gggacgatga ccagaccacg gcgcaggcga tcgacccggc gggctggatg cacagcggcg
     3061 acctggcggt gatcgatgcc gacggctact gcaacatcgt cggccggatc aaggacatgc
     3121 tgatccgcgg cggggagaac gtgtaccccg ccgaagtcga ggagttcctc gccaggcatc
     3181 cgaaggtgca ggcggtgcag gtgttcggcg tgccggaccc gcggctcggc gaggacgtgt
     3241 gcgcgtggat cgtgctcaaa cccggcgtca tcgccaccga cgaagagatc cgcaactggt
     3301 gccagggccg cgtcgcccac tacaaggtgc cgcgccacat ccgcttcgtc cccgcgtttc
     3361 ccgccaccgc caccggcaag ccgcagaagt tcgcgatgcg cgagcagatg atgcgcgagc
     3421 tgggggtccg cattgcggcc tcggcgtaac cgctgctcgc cctcatcccg gagccggcgg
     3481 cgattcgcgg ccgccctggc agcgttgccg gccatgtggc tcgcggccgg ctgcgcagcg
     3541 cgtgcgcagg gcttgcggcg cgacgagcaa gtgctgctgt ttccgagcac ggcgcgcagc
     3601 ctgcccgagg gcggcgggca ggcgcgcatc gaagcctggg tgcacgagct ggagcgccgt
     3661 ccaggcgcga ccgcgctgct cgcgcgctac gtgggcctcg atctgagcga gcttcccgag
     3721 gcggagcgcg cgcgcttcac ggcgcgcacg cagctgttcc gggtcgattc cgagcgcggc
     3781 cgcgtgctgc agatacggtt cgacggcgaa gggctgctgt ccctgcccgc cagcggcgcg
     3841 gacggccgcg tcagcgccgt ggtctcgaac gacgggactg cgcacgcgtc cgacggcccg
     3901 cgctggctcg agtatgcggt gctgatgccc gaggaggatt cgcgcagttt cgccggccgc
     3961 gcgctgtggc tgccgcaagc cggactgagc atcgtctcgg acatcgacga cacgatcaag
     4021 cacacacagg tgcgcgaccg gcgcgagatg ctgctcaaca ctttcgcgcg cgacttcgaa
     4081 gccgtgcccg gcatgccggc ctggctgcag cgctgcgccg ctgccgagcc ggcggcgagc
     4141 tttcactacg tgtcgggcgc tccgcaccag ctctacccgc cgctcgcgga gttcttgcac
     4201 gcgcacgcgt ttccgccggg cacggtgcac ctgcgcagcg tggatctggc gcgcgagatc
     4261 ttcgcggcgg gcggcggcac gcgcacgcac aaactggagg tcataggcca cctgctggac
     4321 gacttcccgc agcggcgctt tgtgctggtc ggggactcgg gcgagcagga cccggagatc
     4381 tatggagagc tggcgcgcac cttccccggc cgcatcgcgg cgatcctgat ccgagacgtg
     4441 acaggcgagc cggccgatgc cgagcgctac gcgcaggcgc tgcgcgacgt gccgcccgcg
     4501 ctgtggagct tgttcaccga gccggatcgg ttgccggccc atgaattctg agccgggggt
     4561 tctgagcccg cttcacgagt ccgcggcctc cccccgcaac cgggcgcgca gtgctgtctt
     4621 gagcaccttg ccgtagttgt tcttgggcag gtcctcgacg aacacgtagc gccgcggccg
     4681 cttgtagcgc gcgatgtgct ccaggcatag ccggtccagc tcctcggccg tgaccgctgc
     4741 gccggcagcg ggaacgacga acgccaccac ctcctcgccc cagtcggctt gcggcgcgcc
     4801 caccacgctc gcctgcgcca cccgcgcatg gcgcagcagc acgtcctcga tctcgcgtgg
     4861 atagatgttc gagccgcccg agatgatcat gtccttggag cggtccttca gcgtgaacca
     4921 gccgcgcccg tcgcggcagc ccacgtcgcc ggtgcgcagc catccgccgc gcagcgcggc
     4981 ctcggtggcg gccgggtcgc gccagtagcc ctgcatcacg cagtcgctgc gggtgacgat
     5041 ctcgcccacc tcgccgtccg gcagatcgcg atccgcctcg tccacgatgc gcaccgccac
     5101 gccggtgcgg gggccgcctg cgctcgccag cgcctcgtgg tcgtgcgccg cgagggcccg
     5161 ctcgtgatcg cgctggctca ggccggtgat ggtcatcggg ctctcgcctt gcccgtagag
     5221 ctggtacagg cgcgggccga acagcgccag cgcgcggacg aggtccggca cgtgcatcgg
     5281 gccgccgccg tagatcacgg tcttcaggtt ggccagttgt gcctgcgcgg ccgcgccgtc
     5341 gttcaccagg cgcatcagca tggtgggggc ggcgaagaac gagacccgga ggtagcgctc
     5401 gatcgcgtcg aacaggtcgc ccggcgtgaa cgactccgag gcggtgatga cgttgtgcga
     5461 gcccttggcg atgtgcggga gcgaatacag gccggagccg tgcgacaggg gcgcggcgtg
     5521 cacgacggtg tcgcgttcgt ccagcgggtc gatgtcggcg tagtaggcgt gcgtggcgaa
     5581 caggaggttg cgctgcgtca gcgtggcgcc tttgggacgg ccggtggtgc cgctggtgta
     5641 gaacagccag gccgggtcgt cgggttgccc ctggtgcggg ggcatcggcg gtgcctgtgc
     5701 caagcgctcg atcagcgccg catcggcttc cagcagcgac gcacgatccg cggtccattc
     5761 gcgccggatg ccgggataca gactcggcga cgcgaagcag gcgcgtaccc ccgaatgctc
     5821 gagaatgaac gcgaactcgc gcggatgcag cttcgcattg atcggcacgg cacacagtcc
     5881 ggcatgccag cagccgtaca tcaactgcag cgtgcccggg ctgttgtgca tggcgatgcc
     5941 gatgcgctcg cccggctgcg cgctcaggcg gccgcgcaga taggcggcgg tgcccgccac
     6001 ccgccgcgag agctcgccgt agcgccacga caggctgccc tgcgtcaccg cgggtgcacc
     6061 cggtcggcac gtcgccgcgg cgtgcagcag cagggacagg ttcatcgctt cagttggcgc
     6121 ccgcgctcag cgtgcgcgtg acgatcagcg cgtggccgtc ggcgcgcgcc aggtacacgg
     6181 gacaggtgag gctgtcgttg ccgcgccaga ccgcgccgcg cgcgctcacg aagggcagcg
     6241 gctcgttgcg ggccggctgg cgcccgccca aagccagcaa cgcaccgaga aagtgcacgc
     6301 cctcgtaggt ggactgtccc agggcgttga gcgtcggagc gcgcagaccg agcagcgccc
     6361 ggtagcgcgc ctggaacgcg agattggcgt cggtgtcgag cccggcaaag taggcgccgg
     6421 cgacgtacag ctcctcggtg caatcggcgt ccagcgccag cagttcgttc tcttcgatcg
     6481 cgcaggacag gcggcggatg cggccgccga ggcggcgcgc ggcgaacacc cggttgaagt
     6541 cgatggcatc ctggccgatc agcgacagca gcaccgcctg tgcgccgctt gcggccagct
     6601 gcgccacggt cggctcgtag tcttcggtgc ccagcggcag gaag
//
LOCUS       DASPGE010000222         8365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_527812, whole genome shotgun sequence.
ACCESSION   DASPGE010000222 DASPGE010000000
VERSION     DASPGE010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527812"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1480)
                     /gene="lplT"
                     /locus_tag="VD791_07160"
     CDS             complement(224..1480)
                     /gene="lplT"
                     /locus_tag="VD791_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HXV07789.1"
                     /translation="MTRGFYTILAAQFFSALADNALLFAAIALLKVLNPACVTESGEC
                     WQTPVLQQFFVFAYIALAPFVGPFADALPKGRVMFISNAVKLSGCLAMLWGMQPLLAY
                     GVVGIGAAMYSPAKYGILTEYLPPQRLVLANGWMEGLTVGAIVLGAILGGFLVGPRFA
                     AFAAHVHVGQAGGWLEGPAKLAIGTIVLLYLIAAAINLYIPRVAIDHRLPKKTPVYIL
                     QDFWHCFRLLWRDPLGQVSLAVTTLFWGAGATLRLIVLTWAATVLNFGPQRAIELTAV
                     TAVGIALGSVLAARFVTLQRAVKVLPVGIAMGFGVMSMVFVSDWRLAVAILMLIGAMG
                     GFFVVPMNALLQHRGHLLMGAGHSIAVQNFNENLSILVLLGAYALMLRAELEIDTITI
                     VFGLLVASVMYLLWRRHRHDQDEGAM"
     gene            1657..2727
                     /gene="alr"
                     /locus_tag="VD791_07165"
     CDS             1657..2727
                     /gene="alr"
                     /locus_tag="VD791_07165"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXV07790.1"
                     /translation="MTRPILASIRISALRSNLALARRRAPDARVLAVIKADAYGHGLL
                     RAAHALADADGFAVLELDDAMRLRAAGFDRRILMLEGFFDAAELPVFAEERLSCVLHN
                     DEQLAVLQAARLPAAVDVFVKIDTGMNRLGFRPEALRRVLDALRACRSAGELTLMTHF
                     ACADDARGIDWQLDAFRRASAGLALPLSLANSATLLRYPHAAQGWARPGIMLYGASPF
                     AEESAHALGLQPAMTLASRLIAVRELAAGESVGYGALFQARAPMRIGVAACGYADGYP
                     RHAPTGTPVLVAGVRTGLVGRVSMDMLCVDLTGLPQAQVGAPVVLWGEGLPVEEVAQA
                     AGTISYELLCALAPRVPVVEVE"
     gene            2779..4137
                     /gene="radA"
                     /locus_tag="VD791_07170"
     CDS             2779..4137
                     /gene="radA"
                     /locus_tag="VD791_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HXV07791.1"
                     /translation="MAKPKTIYSCTECGGQTLKWQGQCPHCSGWNTLVETLAERKPAR
                     YEALAQASRVQPLAEMDAAEEPRRATGIGELDRVLGGGLVRGGVVLLGGDPGIGKSTL
                     LLQALSQLSAVCATLYVSGEESAQQVALRARRLGLPSPGLKVLAEIQLEKILAALGAD
                     KPDVAVIDSIQTLYSEALQSAPGSVAQVRECAAQLTRFAKTAGTALLLVGHVTKEGAI
                     AGPRVLEHMVDSVLYFEGDTHSSFRLVRAIKNRFGAVNELGVFAMTERGLRGVSNPSA
                     LFLSHHGTRVAGSCVLVTQEGTRPLLVEIQALVDDAHSPNPRRLTVGLEQNRLALLLA
                     VLHRHAGIAAFDQDVFVNAVGGVKIDEPAADLAIVLAIVSSLRNRPLPQRLVVFGEVG
                     LAGEVRPVQRGQERLREAAKLGFTHAVIPKANLPKTPIEGIETLGVERVEDAVARMIR
                     DA"
     gene            complement(4159..5481)
                     /locus_tag="VD791_07175"
     CDS             complement(4159..5481)
                     /locus_tag="VD791_07175"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV07792.1"
                     /translation="MRDASHQLGRYELLAELGQGAMGVVYKARDPVLDRVVAIKTINL
                     TLPKDELADYEARFYQEARAAGGLNHPNIVTIYDIGKSERLAYMAMELLEGEELRSIL
                     STKEPLPVSLALDIAIQVADGLAYAHERQVVHRDIKPANIMIVRDGLVKITDFGIARM
                     RTNEVKTMTGMILGSPKYMSPEQVTGKRADHRVDLFSLGVVLYEMLTGEAPFVADSIH
                     GIMYQIMQFTPPPPSTKQPGLPEVLDLIVAKALNKSPDERYQSAMEFARDLRDCKNML
                     EGKSTGGVATADLAAGQAAGARKPERLAPVKTPVGGSEPEEARGLALAKSFDSYEATM
                     RLAALTGVEQEMEVFSNTQAMRRPDLQQPPPPAAPAARAEPGPAPATAPRRTEAPVVA
                     VAPRRAEAAVAAGAPRRTEARTATPTYVYLWVLSGIALAAALILYLMR"
     gene            complement(5495..5905)
                     /locus_tag="VD791_07180"
     CDS             complement(5495..5905)
                     /locus_tag="VD791_07180"
                     /inference="COORDINATES: protein motif:HMM:NF038027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HXV07793.1"
                     /translation="MVRGSGGVVASLLASLLAGALLTACQSALVQEFPLTKFKAERML
                     DEGIREYDDGNYKVASRTIQGALDAGLTTRSQGRAHKYLAFMHCVAGQPAQCRDQFRK
                     GLAADRRLDLTAAESGHPIWAPVFAEVKAGMPKP"
     gene            complement(5914..7398)
                     /locus_tag="VD791_07185"
     CDS             complement(5914..7398)
                     /locus_tag="VD791_07185"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HXV07794.1"
                     /translation="MHKVSALGTATARQAPPAEARVVEFPGTPERAGRTFVCSVLFLD
                     IVEYSKRPVAEQLQIKERFNAQVSQTIAEIPPQDRIILDTGDGVAISFLEDPEDALRV
                     ALTLTHWFALPGGIPVRAGINLGPVRLTRDINGQPNIIGDGINVAQRVMSFAQPGQLL
                     VSRAFHEGVARFAESYATLFSYQGSRTDKHVREHEIFELSAAAGQALEVAERRKRTPR
                     REGGPPAATIAAGARLMGQRRLAFAVTGLSLAALGAALVAFLQPDGGEPQEPATVVAH
                     APAQPSAMPAVPGVPAAVQPESAQQPAQGDEPQRNEPQPAESLRAEPQPAEAQPAEVQ
                     PAEQAPQGAASGKPEQAPQRSGPSRLAAVAPERVADAAPAPAPRVPREPAASDADADT
                     KTVQAPWNPPASSADPVLPVPVTKVRTATGATALVLLAISPWGEVYVNGKSVGVSPPL
                     AEVELKPGKHRIEVRNGSFKPYQEDMELGSYQTVRIKHKFTHAR"
     gene            7612..>8365
                     /locus_tag="VD791_07190"
     CDS             7612..>8365
                     /locus_tag="VD791_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXV07795.1"
                     /translation="MTAANPYELGLDRNPANYVPLSPLSFLRRAAAVYPERQAVVHGA
                     HRTTWRETYARCRRLASALSRHGVGRGDTVAVIAPNVPALFEAHFGVPMTGAVLNALN
                     TRLDATAIAFMLQHGGARVLIADRELSAVVDQALRLTERRPLVIEIDDPLAGGGELLG
                     EATYEDFIAGGDPEFAWSLPPDEWDAIALNYTSGTTGNPKGVVYHHRGAYLNALGNVL
                     VWDMGQHPTYLWTLPMFHCNGWCFPWTVTAMAG"
BASE COUNT         1194 a         2897 c         3014 g         1260 t
ORIGIN      
        1 cacctgcggc ggcatttccc ctcggccggt ggcggcgaga acgagctgcc cgaccggccg
       61 gcgctgctgt gaaaaacgcg gtgctcccac cacctcggac tgttccccgc ctctcacgag
      121 gcggggtgcc ggcgcagccg gcggggcggt gtggtgttac gtgagcagaa ccaccccgtc
      181 gcgcttcgcg cgccacccct ccttggaaag gaggggaacg cgattacatc gcgccttcgt
      241 cctgatcgtg ccggtgcctg cgccacagca ggtacatcac gctcgccacc agcaggccga
      301 acacgatggt gatggtgtcg atctccagct ccgcacgcag catcagcgca taggcgccca
      361 gcagcaccag gatgctcagg ttctcgttga agttctgcac cgcgatggag tggcccgcgc
      421 ccatcagcaa gtggccgcgg tgctggagca gcgcgttcat cggcaccacg aagaagccgc
      481 ccatcgcgcc gatcagcatc aggatcgcca ccgccagcct ccagtcgctg acgaacacca
      541 tgctcatcac gccgaagccc atcgcgatgc ccaccggcag caccttgacg gcgcgctgca
      601 gggtgacgaa gcgcgccgcc agcaccgagc ccagcgcgat gcccacggcg gtgacggcgg
      661 tgagctcgat cgcgcgctgc gggccgaagt tgaggacggt cgcggcccag gtcaggacga
      721 tcaggcgcag cgtggcgccg gcgccccaga acagcgttgt caccgccagc gatacctgcc
      781 cgagcggatc gcgccacagc aggcggaagc agtgccagaa gtcctgcagg atgtacaccg
      841 gcgtcttctt gggcaggcgg tggtcgatcg ccacgcgcgg gatgtacaga ttgatcgccg
      901 cggcgatcag gtacagcagc acgatcgtgc cgatggcgag cttcgccggg ccctcgagcc
      961 agccccccgc ctgcccgacg tgaacgtgtg cggcgaaggc ggcgaagcgc ggcccgacca
     1021 ggaagccgcc gaggatcgcg cccaggacga tggcgccgac cgtcagcccc tccatccagc
     1081 cgttggccag caccagccgc tgcggcggca ggtattcggt aagaatgccg tacttggccg
     1141 gcgagtacat ggccgcgccg atgccgacca cgccgtaggc gagcagcggc tgcatccccc
     1201 acagcatggc gaggcagccg gagagcttca cggcgttgct gatgaacatc acgcgcccct
     1261 tcggcaacgc gtcggcgaac ggccccacga acggcgccag cgcgatgtag gcgaacacga
     1321 agaactgctg caggaccggg gtctgccagc attctccgga ctcggtcacg caggccggat
     1381 tgaggacttt gaggagcgcg atggcggcga acagcagggc attgtcggcc agcgcggaga
     1441 agaattgcgc cgcgaggatg gtgtagaagc cgcgggtcat cgcgtcgtgg tggggatcgg
     1501 cgccgcgggc tgtccccgag ggcgcgcctt tataccacga aaccgcgcgc gcccagcccg
     1561 ccgccggaaa cctgcggcgc tgttgcgccg ggtgagcggt cttggcagac ttgccgtgcg
     1621 ccgcctcgcg gtatgcgcgc gcgacttctc gctgaaatga cccgcccgat cctcgcctcg
     1681 atccggatct ccgctctgcg cagcaacttg gcgctggcgc gccgccgcgc gccggatgcg
     1741 cgcgtgctgg cggtgatcaa ggccgacgct tacggccacg gcctgctgcg cgcggcgcat
     1801 gcgctcgccg atgccgacgg cttcgccgtg ctggagctcg acgacgcgat gcggctgcgg
     1861 gccgcgggct tcgatcggcg catcctgatg ctggagggct tcttcgacgc cgccgagctg
     1921 ccggtgttcg ccgaagagcg cctgagctgc gtgctgcaca acgacgagca gctggccgtg
     1981 ctgcaggccg cccggctgcc cgccgccgtg gacgtgttcg tgaagatcga caccggcatg
     2041 aaccgcctgg gcttcaggcc tgaggcgctg cggcgcgtgc tggacgcgct gcgcgcctgc
     2101 cgcagcgcgg gcgagctcac gctgatgacg catttcgcct gcgccgacga tgcgcgcggg
     2161 atcgactggc agctcgatgc gttccggcgt gccagcgccg gcctcgcgct gccgctgtcg
     2221 ctcgccaact cggccacgct gctgcgctat ccgcacgcgg cgcagggctg ggcgcggccg
     2281 ggcatcatgc tctacggggc gtcgccgttc gccgaggaga gcgcgcatgc gctcgggctg
     2341 cagccagcga tgacgctggc gagccggctc atcgccgtgc gcgagctggc ggccggtgag
     2401 agcgtcggct acggcgccct gttccaggcg cgcgcgccga tgcgcatcgg cgtggccgcc
     2461 tgcggttacg cggacggcta tccgcgccac gcgccgaccg gcacgccggt gctggtggcc
     2521 ggcgtgcgca ccggcctcgt cggccgcgtg tcgatggaca tgctgtgcgt ggacctcacc
     2581 gggctgccgc aggcccaggt cggcgcgccg gtggtgctgt ggggcgaggg cctgccggtg
     2641 gaggaggtgg ctcaggccgc cggcaccatc agctacgagc tgctgtgcgc gctggcgccc
     2701 cgcgttccgg tggtggaggt cgaatgaagt gtgcctgtgg tgcaggtcgt gtgagccggc
     2761 gcgttgcgcg acagcccgat ggcgaagccg aagacgatct atagctgcac cgagtgcggc
     2821 gggcagacgc tgaagtggca gggccagtgc ccgcactgca gcggctggaa caccctggtc
     2881 gagacgctcg ccgagcgcaa gccggcgcgc tacgaggcgc tcgcccaggc gagccgcgtg
     2941 cagccgctcg cggagatgga tgctgccgag gagccgcggc gcgccaccgg catcggcgag
     3001 ttggaccgcg tgctcggcgg ggggctggtg cgcggcggcg tggtgctgct cggcggcgat
     3061 cccggcatcg gcaagtccac gctgttgctg caggcgctgt cgcagctctc cgcggtctgc
     3121 gcgacgctct acgtgagcgg cgaggagtcg gcgcagcagg tggcgctgcg cgcacgtcgc
     3181 ctggggctgc catcgcccgg cctgaaggtg ctggcggaga tccagctgga gaagatcctc
     3241 gccgcgctcg gcgccgacaa gccggacgtg gcggtgatcg actcgatcca gacgctgtac
     3301 tccgaggcgc tgcagtccgc gccgggcagc gtggcgcagg tgcgcgagtg cgccgcgcaa
     3361 ctcacgcgct tcgccaagac cgccggcacc gcgttgctgc tggtcgggca cgtcaccaag
     3421 gaaggggcca tcgccggccc gcgcgtgctc gaacacatgg tggacagcgt gctctatttc
     3481 gagggcgata cgcactccag cttccgcctg gtgcgcgcca tcaagaaccg cttcggcgcg
     3541 gtcaacgagc tgggcgtgtt cgccatgacc gagcgcgggc tgcgcggcgt gagcaacccg
     3601 tccgcgctgt tcctgtcgca tcacggcacg cgggtggccg gttcgtgcgt gctggtgacg
     3661 caggagggca cgcgcccgct gctggtggag atccaggcgc tggtggacga cgcgcattcg
     3721 cccaatccgc gccggctgac cgtggggctg gagcagaacc gcctggcgct gctgctcgcg
     3781 gtgctgcacc gtcacgccgg catcgccgcg ttcgaccagg acgtgttcgt gaacgcggtg
     3841 ggcggagtga agatcgacga accggccgcc gacctcgcca tcgtgctggc gatcgtctcg
     3901 tcgctgcgca accggccgct gccgcagcgg ctggtggtgt tcggggaggt ggggctcgcc
     3961 ggcgaggtgc gaccggtgca acgcggccag gagcgcctgc gcgaggccgc caagctgggc
     4021 tttacccacg ccgtgattcc gaaggcgaat ctgcccaaga cgccgatcga gggaatcgaa
     4081 acgctgggcg tggagcgggt ggaggatgcg gtcgcccgaa tgatccgcga cgcatgactc
     4141 tcaccggtca cgaagatctc agcgcatcag gtacagtatc agcgccgctg cgagcgcgat
     4201 gccgctgagg acccagaggt aaacgtaggt aggcgtggca gtgcgcgctt cggtgcggcg
     4261 cggcgcaccg gcggcgaccg ctgcctcggc gcggcgcggt gcgacagcga cgacaggcgc
     4321 ttcggtgcgg cgcggcgcgg tcgcgggcgc aggccctggc tctgcgcgcg cggccggcgc
     4381 cgcggggggc ggcggctgct gcaggtcggg ccggcgcatc gcctgcgtgt tggagaacac
     4441 ctccatttcc tgctccacgc cggtgagcgc ggccagccgc atggtcgcct cgtaagagtc
     4501 gaaggacttg gcgagcgcca gtccgcgcgc ctcctcgggc tcgctcccgc cgaccggcgt
     4561 cttcaccggc gcgagccgct ccggcttgcg cgcgcccgcc gcctggccgg cggcgaggtc
     4621 ggcggtggcc acgccgccgg tggacttgcc ctccagcatg ttcttgcagt cgcgcaggtc
     4681 gcgcgcgaac tccatcgcgc tctgatagcg ctcgtcgggg ctcttgttca gcgccttggc
     4741 cacgatcagg tccagcacct ccggcaggcc cggctgcttc gtgctcgggg gcggcggcgt
     4801 gaactgcatg atctggtaca tgatgccgtg gatgctgtcg gcgacgaagg gcgcttcgcc
     4861 ggtgagcatc tcgtagagca ccacgcccag cgagaacagg tcgacgcggt gatcggcgcg
     4921 cttgcccgtg acctgctccg gcgacatgta cttcggcgag ccgaggatca tgccggtcat
     4981 ggtcttgacc tcgttggtgc gcatgcgcgc gatgccgaag tcggtgatct tcaccagccc
     5041 gtcgcgcacg atcatgatgt tcgccggctt gatgtcgcgg tgcacgacct ggcgctcgtg
     5101 cgcgtaggcg agcccgtcgg ccacctggat ggcgatgtcc agggccagcg acaccggcag
     5161 cggctccttg gtggacagaa tggagcgcag ctcctcgcct tccaggagct ccatcgccat
     5221 gtaggcgagg cgctcgctct tgccgatgtc gtagatggtg acgatgttcg gatgattgag
     5281 cccgccggcc gcgcgcgcct cctgatagaa gcgcgcttcg tagtcggcca gctcgtcctt
     5341 gggcagggtg aggttgatgg tcttgatcgc caccacccgg tcgagcaccg gatcgcgcgc
     5401 cttgtacacc acgcccatgg cgccctgtcc gagctccgcg agaagctcgt agcggcccag
     5461 ctgatgggag gcgtcgcgca cgctcaccgc ggacttacgg cttcggcatg cccgccttca
     5521 cctccgcgaa caccggcgcc cagatcgggt gcccggattc cgcggcggtg aggtcgagcc
     5581 gccggtccgc ggcgaggccc ttgcgaaact gatcgcggca ctgggccggc tggcccgcga
     5641 cgcagtgcat gaaggcgaga tacttgtggg cgcgcccctg cgagcgcgtg gtgagcccgg
     5701 cgtcgagcgc gccctggatg gtgcgcgagg cgaccttgta gttgccgtcg tcgtactcgc
     5761 ggatgccttc gtcgagcatc ctctccgcct tgaacttggt caacgggaat tcctggacca
     5821 gcgcgctctg gcacgcggtg aggagcgcgc ccgccagcaa cgacgccagc aacgacgcga
     5881 ccactccacc ggatccgcgc accatgaatc gccttatctc gcgtgggtga acttgtgctt
     5941 gatcctgacg gtctggtagc tgccgagctc catgtcctcc tggtagggct tgaagctgcc
     6001 gttgcgcacc tcgatgcgat gcttgccggg cttgagctcc acctccgcca gcggcgggct
     6061 cacgccgacc gacttgccgt tcacgtacac ctccccccag ggggagatcg ccagcaacac
     6121 cagggccgtc gctccggtcg cggtgcgcac cttggttacc ggtaccggca gcacggggtc
     6181 ggcggacgag gccggcgggt tccagggcgc ctgcacagtc ttggtatcgg catcggcgtc
     6241 gctcgctgca ggttcgcgcg gaacccgcgg ggccggcgcg ggcgcggcgt ccgcgacccg
     6301 ttccggagcg accgctgcca ggcggctcgg ccccgatcgc tgcggggcct gctccggctt
     6361 gccggacgcg gcgccctgcg gggcttgctc ggccggttgg acctcggccg gctgagcctc
     6421 ggcaggctgg ggctcggccc gcagggattc ggcaggctgg ggctcgttgc gctggggctc
     6481 gtcaccctgg gcgggttgct gtgcggactc gggctgcacc gccgcaggca cgcccggtac
     6541 cgccggcatc gcgctcggct gcgccggcgc atgcgcgacc acggttgccg gttcctgggg
     6601 ttccccgccg tccggctgca agaaggcgac cagggccgcg ccgagcgcgg ccagtgaaag
     6661 cccggtcacg gcgaacgcga gcctgcgctg ccccatcaga cgcgcgcccg cggcgatcgt
     6721 ggcggccggc ggcccgccct cgcggcgagg cgtgcgtttg cggcgctcgg ccacctccag
     6781 cgcctgtccc gcggccgcgc tcagctcgaa aatctcgtgc tcgcgcacgt gcttgtcggt
     6841 gcgcgagccc tggtaggaga acagcgtggc gtagctctcc gcgaagcgcg cgaccccctc
     6901 gtgaaacgcc cgcgatacca gaagctggcc aggctgcgcg aagctcatca cgcgttgtgc
     6961 gacgttgatg ccgtcgccga tgatgttcgg ctggccgttg atgtcgcgcg tgagccgcac
     7021 cggccccagg ttgatgcccg cgcgcaccgg gatgccgccc ggcagcgcga accagtgggt
     7081 cagcgtcagc gcgacgcgaa gcgcgtcctc ggggtcctcc aggaaggaga tcgccacccc
     7141 gtcgccggtg tccaggatga tgcggtcctg cggcgggatc tcggcgatcg tctgcgagac
     7201 ctgggcgttg aagcgctcct tgatctgcag ctgctcggcg accggtcgct tggaatactc
     7261 gacgatgtcg aggaacagca cgctgcacac gaaagtgcgc ccggcgcgct cgggggttcc
     7321 ggggaactcc accacgcgcg cctccgcggg cggcgcctgc cgcgccgtgg ccgtgcctag
     7381 ggccgacact ttgtgcattc ggggcagaaa aaaagactgg aaatcaatcg gttcttgtag
     7441 cgtaccggct tcggcgccgg gccccgcggc ggccgcgccg agcgctgccc gcatgcagta
     7501 tcattcggcc caggccagcc aatcgacacc agaaagcaaa aaaaaagccc gcagtttcga
     7561 gcgcttcgaa gcgtgcgcag cgcaccgcag aacccctcga ggagagcgca ggtgaccgca
     7621 gccaatccct acgagctcgg cctggatcgc aatccggcca actacgtgcc gctgtcgccg
     7681 ctgtcgttcc tgcgccgggc cgcggcggtg tatcccgagc ggcaagcggt ggtccacggg
     7741 gcgcaccgca ccacctggcg cgagacctac gcgcgctgcc ggcggcttgc ttccgcgttg
     7801 tcgcggcacg gcgtcggccg cggcgacacc gtggcggtga tcgcgcccaa cgtgccggcg
     7861 ctgttcgagg cgcacttcgg cgtgccgatg accggcgccg tgctcaacgc cctgaacacc
     7921 cgcctcgatg ccacggccat cgccttcatg ctgcaacacg gcggcgcgag ggtgctgatc
     7981 gccgaccgcg aactgtcggc ggtggtggac caggccctgc gcctgaccga gcggcgcccg
     8041 ctggtgatcg agatcgacga cccgctcgcc ggcggcggtg agttgctggg cgaggccacc
     8101 tacgaggact tcatcgccgg cggcgacccg gagttcgcct ggagcctgcc acccgacgaa
     8161 tgggacgcga tcgcgctcaa ctacacctcg gggacgaccg gcaaccccaa gggggtcgtc
     8221 tatcaccatc ggggcgcgta cctgaacgcg ctcggcaacg tcctcgtctg ggacatgggc
     8281 cagcacccga cgtacctctg gacgctgccg atgttccact gcaacggctg gtgcttcccc
     8341 tggacggtga cggccatggc gggga
//
LOCUS       DASPGE010000223         2699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_879846, whole genome shotgun sequence.
ACCESSION   DASPGE010000223 DASPGE010000000
VERSION     DASPGE010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879846"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VD791_07195"
     CDS             <1..869
                     /locus_tag="VD791_07195"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV07796.1"
                     /translation="LQDEITAQIIGAIAGSYGAISRTRRDTAGTANLEAYDCVLQAYA
                     YERTLTAAVHAATRDCLERAVKLDPAYAAAWSKLAFLYADEYAFGYNPRPGSLDRALD
                     VAQRAVELDPSSDMAHWHLARTYFWRNEVEEAVTEAERALALNPNNAFVLAAAGTYMA
                     PTGGNNLKRGVQLASKALRIDPNPPGWYYVPQILFHYEKREYEAALAASLKMNMPKFF
                     HTYVWRSAIYGQLGRGADAEGSRAKLLRLYPTYPENARRELKKFNVSPALAEHVVDGL
                     RKAGLEIPEQAH"
     gene            complement(916..1272)
                     /locus_tag="VD791_07200"
     CDS             complement(916..1272)
                     /locus_tag="VD791_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07797.1"
                     /translation="MHPKARYLFIVSMDVAPEREALFNEVYDTEHVPNLLKVPGVLSA
                     RRLTTEPLTVSIGGERKTLVVEGEPRYSAVYELESAEVLLSDAWAKAAEQGRWPTEVR
                     PYTRNRRHVLKKIRGA"
     gene            complement(1281..2201)
                     /locus_tag="VD791_07205"
     CDS             complement(1281..2201)
                     /locus_tag="VD791_07205"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV07798.1"
                     /translation="MTPQALAAEARAESGTPYTRRARYHCAENGFTFTWPPVPARQFL
                     AERDRAFDPRTPTGLIALDIAADLGTAYPATTPALLLRYVKLRAGEQLRTKLVASGEI
                     YYVYSGSGESRNSGDAISWSAGDAFCFPGGGETVHRAGGGDALLILGTNEPLLAFEGA
                     QAPAPGQARVQATHWPAAEIERHFQTVWARPITPNTTGHSVALVSTALESTTTTASIN
                     VAINSLAVGCDQRPHRHNGVAVTFSLQGEGVYSMIDGERVDWSDGAAQITPAARLHSH
                     HNRGSQRMRSLIFQDEGLHYYTRTPGFSFD"
     gene            2273..>2699
                     /locus_tag="VD791_07210"
     CDS             2273..>2699
                     /locus_tag="VD791_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HXV07799.1"
                     /translation="MHARTRIKPPFGLRPLSPNLGAEIQGVDLAQGVDDQLFSAIHQA
                     FLRHQVLLFGAQELPPGKQVEFARHFGEVQIHVMNQYHADGHPELYRLSNLDEQGNPT
                     GHHPDKGTLAWHTDGSWRPVTGQATILYAEIVPEEGGETH"
BASE COUNT          441 a          937 c          889 g          432 t
ORIGIN      
        1 ccctgcagga cgagatcacg gcacagatca tcggcgcgat cgccggatct tacggtgcga
       61 tatcgcgcac ccgccgcgac acagccggta ccgccaatct ggaagcatac gactgtgttc
      121 tccaagcata cgcgtacgaa cgcacgctca cggcggccgt tcatgccgcg acgcgcgact
      181 gcctggagcg cgcggttaag ctcgacccgg cttacgccgc tgcctggtcg aaattggctt
      241 tcctttacgc cgacgagtat gcgtttggct acaatccacg ccccgggtct ctggatcgtg
      301 cactggatgt ggcgcagcgc gccgtcgaac tggaccccag tagcgacatg gctcattggc
      361 atctcgcacg cacctacttc tggcgcaacg aggtcgagga agcagtcacc gaggcggagc
      421 gcgcgctggc gctcaacccc aacaacgctt tcgtgctcgc cgctgcgggc acctacatgg
      481 cgccgaccgg tgggaacaat ctcaagcgcg gagtacagtt agcgtctaag gcgttgcgga
      541 tcgatcccaa cccgccaggc tggtactacg tgcctcagat cctgtttcac tacgagaaac
      601 gcgagtacga ggcggcgctg gccgcatctc tgaagatgaa catgccgaag ttcttccaca
      661 cctacgtgtg gcgatcggcg atctacggcc aattagggcg cggcgccgac gcggagggct
      721 cgagagccaa acttctgcgg ctctacccga cctacccgga aaacgcgcgt cgcgagctga
      781 agaaattcaa tgtcagcccg gccctggccg agcacgtcgt cgacggcctg cgcaaggcgg
      841 gcctggagat ccccgagcag gctcattgag tcgcggcctc gcgacccacg tccggggccg
      901 gctgcggagc gacgcctacg cgccccggat cttcttgagc acgtggcgcc ggttacgcgt
      961 gtaggggcgc acctcggtcg gccaacggcc ctgctcggcc gccttggccc aggcgtcgct
     1021 gagcagcacc tcggcgcttt ccagctcgta gaccgcgctg tagcgcggct cgccctcgac
     1081 gaccagcgtc ttgcgctcgc cgccgatgga cactgtcagc ggttcggtgg tgaggcgccg
     1141 cgcggacagg acgcccggca ccttgagaag attgggtacg tgctcggtgt cgtacacctc
     1201 gttgaacagc gcctcgcgct cgggcgcgac gtccatgctg acgatgaaca gatagcgcgc
     1261 cttggggtgc atgacgccgc tcaatcgaag ctgaagccgg gcgtgcgcgt gtagtagtgc
     1321 agcccttcgt cctggaatat cagcgagcgc atgcgttggc tgccgcggtt gtgatgcgag
     1381 tgcagccgcg ccgcgggcgt gatctgcgcc gcgccgtcgg accagtccac acgctcgccg
     1441 tcgatcatcg agtacacgcc ctcgccttgc agcgagaacg tcaccgccac accgttgtgg
     1501 cgatgcgggc gctgatcgca gccgaccgcc aggctgttga tggcgacgtt gatcgacgcg
     1561 gtggtggtgg tactctccag cgccgtgctg acgagcgcca ccgagtggcc ggtggtgttg
     1621 ggcgtgatcg gccgcgccca caccgtctgg aagtggcgtt cgatctccgc cgccggccag
     1681 tgcgtggcct gcacccgcgc ctggccaggc gcgggcgcct gcgcgccctc gaacgcgagc
     1741 agcggctcgt tcgtccccag gatcagcagc gcatctccgc cccccgcccg atgcacggtc
     1801 tcgcccccgc cggggaagca gaacgcgtcg cccgcactcc aagaaatggc gtcgccgctg
     1861 ttgcggctct cgccgctacc ggaatacacg tagtagatct cgcccgatgc cacgagcttt
     1921 gtacgcagct gctcgcccgc gcgcagcttg acgtagcgca gcagcagcgc aggcgtggtg
     1981 gccggatagg cggtgcccag atcggccgcg atgtccaggg cgatcaggcc ggtgggcgtg
     2041 cgcgggtcga aggcccggtc gcgctcggcc aggaactggc gcgccggcac cggcggccag
     2101 gtgaaggtga agccgttctc ggcgcagtgg tagcgggcgc gccgggtgta gggcgtgccc
     2161 gattcggccc gggcttcggc ggcgagggct tgcggcgtca ttcgggcttc ctccgtggtt
     2221 cgcgcaagcg gctaatatag tccaaacgcc gctcccgctg ccgaccgccc gcatgcacgc
     2281 ccgcacccgc atcaaaccgc ccttcggtct gcgcccgctc agccccaacc tcggcgccga
     2341 gatccagggc gtggatctcg cgcaaggcgt ggacgaccaa ctgttcagcg cgatccacca
     2401 ggcgttcctg cgccaccagg tgctgctgtt cggcgcccag gagctgccgc ccggcaagca
     2461 ggtcgagttc gcccgccatt tcggcgaggt gcagatccac gtcatgaacc agtatcacgc
     2521 cgacggccac cccgagttgt accggctttc gaacctggac gagcaaggca accccaccgg
     2581 gcaccatccc gacaagggca cgctggcctg gcacaccgac ggctcgtggc gtccggtcac
     2641 cggccaggcc acgatcctgt acgccgagat cgtcccggaa gaaggcggcg agacccact
//
LOCUS       DASPGE010000224         2004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_762279, whole genome shotgun sequence.
ACCESSION   DASPGE010000224 DASPGE010000000
VERSION     DASPGE010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762279"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="VD791_07215"
     CDS             complement(<1..1446)
                     /locus_tag="VD791_07215"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV07800.1"
                     /translation="MSSAASSVLGRLLRSSPLRHRRFRAFYFGSVGTALGYTMQATIA
                     AWLMATLTPSALMVALVQTASATPSLLLGLAAGTLADIVDRRRVLLASQILLLGATAA
                     LGAVAVAGLAGPATLLALTFLIGVGFTFYMPTQQAMINDLVPHAELPPAALLGAAAFN
                     VSRAVGPALAGAMAAWIGSGYALLASALFFGGMIVAIRGMRRTAPASFGIPETVVSGV
                     ASGLRYARHSPPLRALLLRVVSFSFCASALFALLPVVARDLLGMGAGGFGVLFACFGA
                     GAVAAAFVVPRRLQRVSLNSVVNSGFVLWAAATALVTSTSILAVAMVGAVGAGAAWVS
                     ALASLSAGTQSSTPAWVRARALAMNLVAFQAAMAVGSALWGWLASAAGLRITLALSAA
                     AALLLLALNQRARVALGHESALRTGVQLPELAVVSEPLPDDGPVLIQMEYRVDPENRA
                     AFLQAIQDVEPARRRNGASAWRVFRDLEDDTR"
     gene            complement(1439..>2004)
                     /locus_tag="VD791_07220"
     CDS             complement(1439..>2004)
                     /locus_tag="VD791_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV07801.1"
                     /translation="SAPGRVGLHQVNLLDAEATRACFAGIGRVDVLCNIAGGFTMGDP
                     VHAAGDEVWTRMFDLNVRTLVNSVRAVVPGMIERKRGKIVNVGAVAGLGGGLGGQANM
                     GAYSAAKSAVIRITESMAAELKRKGINVNCVLPSIIDTPQNRAAMPKADPAQWVAPAD
                     LANVILFLASEEARAVHGAALPVVNLV"
BASE COUNT          328 a          713 c          731 g          232 t
ORIGIN      
        1 gcgggtgtcg tcctccaggt cgcggaacac gcgccaggcg ctcgccccgt tgcggcgcct
       61 ggccggctcg acgtcctgga tcgcctgcag gaaggcggcg cggttctcgg ggtccacgcg
      121 gtactccatc tggatcagca ccgggccgtc gtcggggagc ggctcgctca ccaccgccag
      181 ctcgggaagc tgcacgccgg tccgcagcgc ggactcgtgc ccgagcgcca cgcgcgcgcg
      241 ttgattgagg gcgagcagca gcagggcggc ggcggctgac agcgcgagcg tgatgcgcag
      301 gccggcagcc gaggcgagcc acccccacag cgcgctgcct accgccatgg cggcttgaaa
      361 cgccaccagg ttcatcgcca atgcgcgcgc acgtacccaa gccggtgtag agctttgggt
      421 gccggccgac aggctggcga gcgcgctcac ccaggccgcg cccgcgccga ccgcgccgac
      481 catggcgacg gcgaggatcg atgtagaggt gacgagcgcg gtggctgccg cccacagcac
      541 gaagccgctg ttcaccacgc tgttcaggga cacgcgctgc aagcggcgcg gaacgacgaa
      601 ggccgccgcg accgcgccgg cgccgaagca ggcgaacagc acgccgaagc cgccggcgcc
      661 catgccgagc agatcgcgcg cgaccaccgg cagcagcgca aacagcgcgc tggcgcagaa
      721 gctgaagctc accacgcgca ggagcagcgc gcgcagcggg ggagaatgcc gcgcatagcg
      781 caggccgctt gccacgcccg agacgaccgt ctccgggatg ccgaagctgg cgggggcggt
      841 cctgcgcatt ccgcggatcg cgacgatcat gccgccgaag aacagcgcgc tcgccagcaa
      901 ggcgtagccc gaaccgatcc acgcggccat cgccccggcc agcgccggtc ccaccgcacg
      961 cgacacgttg aaagcggcag ccccgagcag cgccgccggc ggcagctccg cgtgcggcac
     1021 caggtcgttg atcatcgcct gctgggtcgg catgtagaag gtgaagccga ccccgatcag
     1081 gaacgtgagc gcgagcagcg tcgccggccc agccaggccg gcgacggcca ccgcgccgag
     1141 cgcggcggtg gcgccgagca gcaggatctg ggaagcgagc agcacgcggc gtcgatcgac
     1201 gatgtcggcg agcgtgccgg ccgccagtcc gagcagcagg gaaggggttg cggaggcggt
     1261 ctgcaccagc gccaccatca gcgcggacgg cgtcagcgtg gccatcaacc aggcggcgat
     1321 ggtggcctgc atggtgtagc ccagcgcggt gcccaccgag ccgaaataga acgcgcggaa
     1381 gcgccggtgc cgcagcggcg aggaccgtag caggcggcct aggaccgagg aggcggcgct
     1441 agacaagatt cacgaccggc agcgcggcac cgtgcaccgc gcgcgcttcc tccgacgcca
     1501 ggaacaggat cacgttggcg aggtccgccg gtgcgaccca ctgggcggga tccgccttcg
     1561 gcatcgcagc gcggttctgc ggggtgtcga tgatgctggg tagcacgcag ttgacgttga
     1621 tgcccttgcg cttcagctcc gctgccatgc tctcggtgat gcggatcacc gcgctcttcg
     1681 ccgccgaata ggcgcccatg ttggcctggc cgccgagccc gccgcccagg ccggcgacgg
     1741 cgccgacgtt gacgatcttg ccgcgcttgc gctcgatcat gccgggcacc acggcgcgca
     1801 cgctgttcac cagcgtgcgc acgttgaggt cgaacatccg cgtccagacc tcgtcgccgg
     1861 cggcgtgcac cggatcgccc atggtgaagc cgccggcgat gttgcacagc acgtcgacgc
     1921 ggccgatgcc ggcgaagcag gcgcgtgtgg cctcggcgtc gagcaggttg acctggtgaa
     1981 ggccaaccct cccgggcgcg gacg
//
LOCUS       DASPGE010000225         6873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_762455, whole genome shotgun sequence.
ACCESSION   DASPGE010000225 DASPGE010000000
VERSION     DASPGE010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762455"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..564)
                     /locus_tag="VD791_07225"
     CDS             complement(37..564)
                     /locus_tag="VD791_07225"
                     /inference="COORDINATES: protein motif:HMM:NF014543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07802.1"
                     /translation="MNLASLKSVILLLAGAAFATATVAAPEDDVQWIADGNGCKVANP
                     FPQPDESITWTGPCKDGLADGDGVLTFFLGDREHSRYQGTLRKGWAQGRGTLLHPDGS
                     RYEGEWARSMENGLGRREWPDGSWYEGGWRNGRPHGQGQFRRPDGKIFIGEWVDGVYE
                     GDLEPEEERLDPNRT"
     gene            complement(622..1113)
                     /locus_tag="VD791_07230"
     CDS             complement(622..1113)
                     /locus_tag="VD791_07230"
                     /inference="COORDINATES: protein motif:HMM:NF014543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07803.1"
                     /translation="MRGPTFAMKMLILHGLALTLAVAPLHAADMTYIKDKKGCKIGNP
                     TPKPKETVTWSGRCEGGFAQGKGILQFYLDGKPGARYEGELKKGLMEGSGELRTTDGA
                     VYDGDWLAGKPDGYGKYTAPDGSTFVGGWTAGKQDGPGTLRNKDGSGLVGRWKDGQYV
                     GPR"
     gene            complement(1143..2069)
                     /gene="argF"
                     /locus_tag="VD791_07235"
     CDS             complement(1143..2069)
                     /gene="argF"
                     /locus_tag="VD791_07235"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HXV07804.1"
                     /translation="MPHSPRHFLQLKDLSRAELEYLLARARQLKARQRSGTLYQPLRG
                     RTLAMIFEKSSTRTRVSFEAGMAQLGGHALFLTTRETQLGRGETIEDVAEVISRMVDL
                     VMVRTFEQAIVERFAAHSRVPVINGLTDEYHPCQILADIFTYLEHRGPIRGKTVAWVG
                     DSNNVCNTWLQAARVFDFRFHVATPPGYELEPERGEDYESAHLQQFTDPHDAARGADL
                     VTTDVWTSMGFEDEAEARKREFADYRVDTDMMKLAKPDALFMHCLPAHRGEEVEAAVI
                     DGAQSVVWEEAENRLHTQKALMEYLLLGKVES"
     gene            complement(2091..3332)
                     /locus_tag="VD791_07240"
     CDS             complement(2091..3332)
                     /locus_tag="VD791_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXV07805.1"
                     /translation="MTQHLMDTYARLPVAFERGQGVWLWDTRGKRYLDALSGIAVSGL
                     GHAHPRLTQAIAAQAGRILHASNLYRIPEQERLADLLCEISGMDEVFFCNSGCEANEA
                     AIKLARLYGHRRGIEQPTIVVMEKAFHGRTIATLSATGSRKVQAGFEPLLAGFARVPY
                     DDLEALGALAGNRNVVAVLVEPVQGESGVNVPDDGYLARLREMCDRHVWLLMLDEVQT
                     GMGRTGKWFAYQNAPYQSTAYQNTGVRPDVLTLAKGLANGIPIGACLAAGPAAGVFKP
                     GSHGSTFGGNPLACTAAAATLRIIAEEGLLDNAVRIGGLIRAGLRERLGHLPSVREIR
                     GKGLMLGIELDRPCGELVELALERGLLINVTADRVVRLLPPLVMQPEEAGLLIEGLAD
                     LIRERLGATAQAPGEPAGVAG"
     gene            complement(3447..5429)
                     /locus_tag="VD791_07245"
     CDS             complement(3447..5429)
                     /locus_tag="VD791_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXV07806.1"
                     /translation="MRKPVSRILVINDEALVLRELIKGLNAAAKSLDNPLGIVFSGVT
                     TARDALKAIEEDGDMQAVIVDDKLYTLKSESGNHRMLQMSALELVQRVTRFRPELDVY
                     VLIAQEHEDEVVDALFTEAVDGYFYREERDYRGMYRILNAQLQERADTPFYDALKSYV
                     FMAKDAWHTPGHSSGDSLRDSPWASDFYEFMGEHVFDADLSVSVPMLDSLMEPTGVIL
                     EAQKMAAKAFGARRTFFATNGTSTSNKVIFQTLIAPGEKLLLDRNCHKSVHHGVVLSG
                     AHPVYLDSSVNKKYGIYGPIPKKTLLRAIEEHADAEALVLTSCTYDGLRYDLAPIIEA
                     AHAKGIRVVVDEAWYGFARFHPAFRPTALESGADYATQSTHKILSAFSQASMLHVNDP
                     EFNEHIFRENFNMHTSTSPQYSMIASLDVARKQAVMEGYKLLTRTLLLAQELRQQINS
                     TGVFRVLELDDLLPDEVRHDNILLDPTKVTVDISECGYTVEELQHELFERYNIQVEKS
                     TFNTLSLLLTIGTTRSKVSRLYDALMRVARERRAPRRVYRTPDVPGFTHLKYLPRDAF
                     YCGGQAMPILDEQDRIMQSLNGKVAADQITPYPPGIPVLVPGQVITAEILHYLAGLLR
                     SHKRIELHGILYDGYMPCLRVLTPPDEKGLKRIKPA"
     gene            complement(5605..5868)
                     /gene="rpsT"
                     /locus_tag="VD791_07250"
     CDS             complement(5605..5868)
                     /gene="rpsT"
                     /locus_tag="VD791_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HXV07807.1"
                     /translation="MANTAQARKRARQSEKRRALNNAAQSELRTAIKKVRKAIAGGDQ
                     AAAQAALDASRSTIDAMARKGMFHRNTAARHKSRLQAAIKALS"
     gene            6033..>6873
                     /gene="murJ"
                     /locus_tag="VD791_07255"
     CDS             6033..>6873
                     /gene="murJ"
                     /locus_tag="VD791_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HXV07808.1"
                     /translation="MNLLRSLVTVSGMTMISRVLGFVRDAVIARSFGAGLYTDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSQRGEQDTRVFVEHITGLLGLALFLVTALG
                     ILAAPLIVYVSAPGFAAVPEKFDVTVALLRVTFPYILFISLASLAGAILNTYSRFAIP
                     AFTPTLLNLSFIVFALWLAPWFDPPVMALAWAVFLGGLLQLGFQLPYLVRIGLLPRPR
                     LNLRDAGVWRVLKLMGPALLGVSASQVSLLINTIFASFLVAGSVSWLYYADRLMELPA
                     GVLG"
BASE COUNT          958 a         2320 c         2355 g         1240 t
ORIGIN      
        1 actttcttga tcgcgctcat gttgtccgca gcgggctcag gtgcggttgg ggtcgagccg
       61 ctcttcctcg ggctcgaggt cgccttcgta cacgccatcg acccattcgc cgatgaagat
      121 cttgccgtcg ggtcggcgga actgtccctg gccgtgcggg cgcccgtttc tccagccgcc
      181 ttcgtaccag ctgccgtccg gccattcgcg gcggcccagg ccgttctcca tgctgcgcgc
      241 ccactcgcct tcgtagcggc tgccgtccgg gtgcagcagc gttcccctgc cttgcgccca
      301 gcccttcctg agcgtgccct ggtagcggct gtgctcgcgg tcgcccagga aaaaagtcag
      361 caccccgtcg ccgtcggcga gaccgtcctt gcacggaccg gtccaggtga tgctctcgtc
      421 gggctgcggg aacggattgg cgaccttgca gccgttgccg tcggcgatcc attgcacgtc
      481 gtcctcgggg gcggccacgg tggccgtcgc aaaggctgcg ccggcgagca gcaggatcac
      541 ggatttcagt gatgccaagt tcatggagaa tcctctgaga aagtggaaac cttgctccct
      601 gtggttcgac cctcgggaag gtcatcgtgg accgacgtac tgaccgtcct tccagcgccc
      661 gacgagcccg ctgccgtcct tgttgcgcag cgtgccgggc ccgtcctgct tgccggcggt
      721 ccagccgcct acgaaggtgc tgccgtcggg ggcggtgtac ttgccgtagc cgtcgggctt
      781 gcccgccagc cagtcgccgt cgtacaccgc gccatcggtc gtgcgcagct cgccgctccc
      841 ctccatcaat cccttcttca gctcgccctc gtaacgcgcg cccggcttgc cgtccaggta
      901 gaactgcaga atgcctttac cctgggcgaa gccgccctcg caacggccgg accaggtcac
      961 cgtttccttg ggcttgggcg tcgggttccc gatcttgcag cctttcttgt ccttgatgta
     1021 cgtcatatcg gccgcgtgca gcggcgcgac cgcgagcgtc agcgcgaggc cgtggaggat
     1081 cagcatcttc atggcgaagg taggaccccg caccgcgcaa ttggttgccc gcggcgatcc
     1141 gttcacgatt ccactttgcc cagcagcaga tattccatca atgccttttg cgtgtgcagt
     1201 ctgttctccg cttcctccca caccacgctt tgcgcgccat cgatgacagc ggcctcgact
     1261 tcctcgccgc ggtgcgcggg caggcagtgc atgaacaggg cgtcgggctt ggcgagcttc
     1321 atcatgtcgg tgtcgacgcg atagtcggcg aactcgcgct tgcgcgcctc ggcctcgtcc
     1381 tcgaagccca tgctggtcca cacgtcggtg gtcaccaggt cggcgccgcg cgccgcgtcg
     1441 tgcggatcgg tgaactgctg cagatgcgcg ctctcgtagt cctcgccgcg ttcgggctcc
     1501 agctcgtagc cgggcggtgt ggccacgtgg aatctgaagt cgaagaccct ggccgcctgc
     1561 agccaggtgt tgcagacgtt gttcgagtcg ccgacccagg ccacggtctt gccgcgtatc
     1621 gggccgcggt gctcgagata ggtgaagatg tcggccagga tctggcacgg gtggtactcg
     1681 tcggtgaggc cgttgatcac gggcacgcgc gaatgcgcgg cgaagcgctc cacgatcgcc
     1741 tgctcgaagg tgcgcaccat caccaggtcc accatgcgcg agatcacctc ggccacgtcc
     1801 tcgatcgtct cgccgcgccc gagctgggtc tcgcgcgtgg tgaggaacag cgcgtgcccg
     1861 ccgagctgcg ccatgccggc ctcgaacgac acgcgcgtgc gcgtcgagct cttctcgaag
     1921 atcatcgcca gggtgcgtcc gcgcagcggt tggtacagcg tcccgctgcg ctggcgcgcc
     1981 ttgagctggc gcgcgcgggc gagcaggtac tccagctccg cgcggctgag gtccttgagc
     2041 tgcaggaagt gccggggcga gtgcggcacg cgaccctcgg tcgaaagctg tcagccggcg
     2101 acgccggccg gctctcccgg tgcctgcgcc gtcgccccca ggcgctcgcg gatcaggtcg
     2161 gcgagcccct cgatcagcag gccggcctcc tcgggctgca tcaccagcgg cggcagcagg
     2221 cgcaccacgc ggtcggcggt gacgttgatc aacagaccgc gttccagcgc aagctcgacc
     2281 agctcgccgc acggccggtc cagctcgatg cccagcatca ggcccttgcc gcggatctcg
     2341 cgcacgctgg gcaggtgccc caggcgctcg cgcaacccgg cccggatcag cccgccgatg
     2401 cgaacggcat tgtccagcag gccctcctcg gcgatgatgc gcagggtggc ggcggcggcg
     2461 gtgcacgcca gcgggttgcc gccgaaggtg gagccgtggc tgccaggctt gaacaccccg
     2521 gcggccggtc ccgcggcgag acaggcgccg atcgggatgc cgttggcgag ccccttggcc
     2581 agcgtgagca catcgggccg caccccggtg ttctgatagg cggtgctctg atagggggcg
     2641 ttctggtagg cgaaccactt gccggtgcgc cccatgccgg tctgcacctc gtccagcatc
     2701 agcagccaga cgtgccggtc gcacatctcg cgcagccgcg cgagataacc gtcgtcgggc
     2761 acgttgacgc cggattcgcc ctgcaccggc tcgaccagca ccgccaccac gttcctgttg
     2821 ccggccaggg cgccgagcgc ctcgaggtcg tcgtagggga cgcgcgcgaa gccggcgagc
     2881 agcggctcga acccggcctg caccttgcgg ctgccggtgg ccgacaaggt ggcgatggtg
     2941 cggccgtgga acgccttctc catcaccacg atcgtgggct gctcgatgcc gcggcggtga
     3001 ccgtacaggc gcgccagctt gatcgcggcc tcgttcgcct cgcagccgga attgcagaag
     3061 aacacctcgt ccatgccgga gatctcgcac agcaggtcgg cgagccgctc ctgctcgggg
     3121 atgcggtaca gattggaggc gtgcaggatg cggccggcct gcgcggcgat ggcctgcgtg
     3181 aggcgcgggt gggcatgccc caggccgctg accgcgatgc cggagagcgc atccaggtag
     3241 cgcttgcccc gggtgtccca cagccacacg ccctggccgc gctcgaaggc gaccggcagc
     3301 cgggcgtagg tgtccatcag gtgctgcgtc atattttgcc agctccaaaa agcaacacgg
     3361 cggcaaacgc cgccgtggag gagagccgtc tcggctgagc gctagttgta tccgaatccg
     3421 ggcctcgaat caagcaccgg cgacgctcaa gccggtttga tgcgcttgag ccccttctcg
     3481 tcggggggcg tgagcacgcg caggcagggc atgtaaccgt cgtacaggat gccgtgcagc
     3541 tcgatgcgct tgtggctgcg cagaaggccg gcgaggtaat gcaggatctc ggcggtgatc
     3601 acctggccgg gcaccagcac cgggatgccg ggggggtacg gcgtgatctg gtcggccgcg
     3661 accttgccgt tcaggctctg catgatccgg tcctgctcgt ccaggatcgg catcgcctgg
     3721 ccgccgcagt agaaggcgtc gcgcggcagg tacttgaggt gggtgaagcc ggggacgtcg
     3781 ggggtgcggt acacgcgccg cggcgcgcgc cgctcgcgcg ccacccgcat cagcgcgtcg
     3841 tacagccgcg acaccttgct gcgcgtggtg ccgatggtga gcagcagcga cagcgtgttg
     3901 aaggtggatt tctccacctg gatgttgtag cgctcgaaca gctcgtgctg cagctcctcc
     3961 accgtgtagc cgcactcgga aatgtccacc gtgaccttgg tgggatcgag caggatgttg
     4021 tcgtggcgca cctcgtcggg cagcaggtcg tcgagctcga gcacgcgaaa cacgccggtc
     4081 gagttgatct gctggcgcag ctcctgcgcc agcagcagcg tgcgcgtgag cagcttgtag
     4141 ccctccatca ccgcctgctt gcgcgccacg tcgaggctgg cgatcatgct gtactgcggg
     4201 ctggtggagg tgtgcatgtt gaagttctcc cggaagatgt gctcgttgaa ttccgggtcg
     4261 ttcacgtgca gcatgctggc ctgggagaag gccgacagga tcttgtgtgt gctctgggtg
     4321 gcgtagtcgg cgcccgattc cagcgcggtg ggacggaacg ccggatggaa gcgcgcgaag
     4381 ccgtaccagg cctcgtccac caccacccgg atgcccttgg cgtgggccgc ctcgatgatc
     4441 ggcgccaggt cgtagcgcag gccgtcgtag gtgcaggagg tgagcaccag cgcctcggcg
     4501 tccgcgtgct cctcgatggc gcgcagcagg gtcttcttcg ggatcgggcc gtagatgccg
     4561 tacttcttgt tgaccgacga gtccaggtac accgggtgcg cgccggacag caccacgccg
     4621 tggtgcaccg acttgtggca gttgcggtcg agcagcagct tctcgccggg tgcgatcagc
     4681 gtctggaaga tgaccttgtt gctggtcgag gtgccgttgg tggcgaagaa cgtgcggcgc
     4741 gcgccgaacg ccttggccgc catcttctgc gcttccagga tcacgccggt gggctccatc
     4801 agggagtcga gcatcggcac cgacaccgac agatcggcgt cgaacacgtg ctcgcccatg
     4861 aactcgtaga agtcgctcgc ccaggggctg tcgcgcaggc tgtcgcccga ggaatgcccg
     4921 ggcgtgtgcc aggcgtcctt ggccatgaac acgtagctct tcagcgcgtc gtagaatggc
     4981 gtgtcggcgc gctcctgcag ctgggcgttg aggatgcggt acatgccgcg gtagtcgcgc
     5041 tcctcgcggt agaagtagcc gtccaccgcc tcggtgaaca gcgcgtccac cacctcgtcc
     5101 tcgtgctcct gcgcgatcag cacgtagacg tcgagctcgg ggcggaagcg cgtcacgcgc
     5161 tgcaccagct ccagcgccga catctgcagc atgcggtgat tgccgctctc gctcttcagg
     5221 gtgtagagct tgtcgtccac gatcaccgcc tgcatgtcgc cgtcctcctc gatcgccttg
     5281 agcgcgtcgc gcgcggtggt caccccggaa aagacgatgc cgagcgggtt gtccaggctc
     5341 ttggcggcgg cgttcagccc cttgatcagc tcccgcagca ccagcgcctc gtcgttgatc
     5401 accaggatcc ggctgacggg cttgcgcatg gtcgggctct tgcttgggtg caacggcatg
     5461 cccgggctaa cgcgcggccg cgcgccggcc tgtcaccgaa cgcgcgcccc ggaccggccg
     5521 atcgcgctca ggaagacaaa cgctcgacat gcgggaggcg cgggcgagtc tggtaacatc
     5581 gcgctccgcg cgcaatacgg cgtatcagga cagagctttg atggcggcct gcaggcggct
     5641 cttgtgtcgc gcggcggtgt tgcggtggaa catgcccttg cgggccatcg cgtcgatggt
     5701 gctgcgcgat gcgtccagcg cggcctgggc ggcggcctgg tcgccgccgg cgatcgcctt
     5761 gcgcaccttc ttgatggcgg tgcgcagctc ggactgggcg gcgttgttca gcgcccggcg
     5821 tttctcggat tgacgggcgc gcttgcgcgc ctgtgcggtg ttggccatta cggctcccta
     5881 gaagaactgc gaatgcaacg cgcccggccg gtgcccggcg gaacttgcga tcgacgagct
     5941 aaggccttga aaaggggcaa gatgataccg gctgccggag ggcaatgcaa gcgccctgcg
     6001 ggcgcccaca ggccggcccg cggaaggcgg cggtgaacct gctgcgttcg ctcgtcacgg
     6061 tgagcgggat gaccatgatc tcccgcgtgc tgggcttcgt gcgcgacgcg gtcatcgccc
     6121 gcagcttcgg ggcggggctg tacaccgacg cgttcttcgt cgccttcaag ctgcccaacc
     6181 tgctgcgtcg catcttcgcc gagggtgcgt tctcgcaggc cttcgtcccg atcctggccg
     6241 agtacaagag ccagcgcggc gagcaggaca cgcgcgtgtt cgtggagcac atcaccggct
     6301 tgctcggact ggcgctgttc ctggtgacgg cgctgggcat cctggccgcg ccgctgatcg
     6361 tgtacgtgag cgcgcccggc ttcgccgccg tgcccgagaa gttcgacgtc accgtcgcac
     6421 tgctgcgggt gacctttccc tacatcctgt tcatctcgct cgcctcgctc gccggcgcga
     6481 tcctcaacac ctacagccgc ttcgccattc ccgcgttcac gcccacgctg ctgaacctgt
     6541 cgttcatcgt gttcgcgctg tggctggcgc cgtggttcga cccgccggtg atggcgctcg
     6601 cctgggcggt gttcctcggc ggattgctgc agctcggctt ccagctgcct tatctggtcc
     6661 ggatcgggct tctgccgcgg ccgcggctca acctgcgcga tgcgggcgtg tggcgcgtgc
     6721 tcaagctcat ggggccggcg ctgctcggcg tgtcggccag ccaggtctcg ctcctcatca
     6781 acaccatctt cgcgtcgttc ctcgtcgccg ggagcgtctc gtggctgtac tacgccgacc
     6841 ggctgatgga gcttcccgca ggcgtgctgg ggg
//
LOCUS       DASPGE010000226         3411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_996945, whole genome shotgun sequence.
ACCESSION   DASPGE010000226 DASPGE010000000
VERSION     DASPGE010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996945"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /gene="glpK"
                     /locus_tag="VD791_07260"
     CDS             complement(<1..1022)
                     /gene="glpK"
                     /locus_tag="VD791_07260"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HXV07809.1"
                     /translation="MRYVLALDQGTTSSRALLFDRAGEPSGLAQRETTQSYPQPGWVE
                     QDPEEIWQTQLAVAREAIEHAGATVQDIAALGITNQRETTVVWERATGRPVYPAIVWQ
                     DRRTAALCEQLKQQGLEALFTGRTGLLLDPYFSGTKVRWILDHVPGARARAEAGELAF
                     GTIDTWLVWKLSGAHLTDASNASRTLLFNIHAGDWDDKLLQLMRVPRAMLPQVVASTG
                     TVAETDAALFGRALPIAGIAGDQQAALFGQRCVTPGMVKNTYGTGCFMLMHTGERSVD
                     SHNRLLTTVAWQQAGRREFALEGSVFVAGAAVQWLRDGLCLIRSADEVEAVAASVPDT
                     GGVYLVP"
     gene            1106..2134
                     /locus_tag="VD791_07265"
     CDS             1106..2134
                     /locus_tag="VD791_07265"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HXV07810.1"
                     /translation="MPVKTTGPADGFAAALNRMGLLQPGETPRIRRLPGGVSSDIVRV
                     DLARGPVCIKRALPRLKVAADWRAPVERNRWEVAWMRVVARVEPDAVPAVLGEDVDGG
                     MFAMAYLDEAQHPVWKARLRDGHIDAAFAAEVGRRVAHIHAATAGDAAVHERFATDHI
                     FHPIRIEPYLVATGRAHPDLAPRLDALARDTAHTRLALVHGDVSPKNILCGPRGPVFL
                     DAECAWYGEPAFDLAFCLNHLLLKCLWRPRWSARYLECFDALAAAYLEGVTWEPRARI
                     ETRTAHLLPGLFLARVDGKSPVEYVTQDWQRSAVREVARGLLESPVDNVGVIRDVWEA
                     RCAGFPGG"
     gene            2214..>3411
                     /gene="eno"
                     /locus_tag="VD791_07270"
     CDS             2214..>3411
                     /gene="eno"
                     /locus_tag="VD791_07270"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HXV07811.1"
                     /translation="MLTTITSVKGRRVWDSRGRPTVEAEVRLAGGAIGRAIAPAGASR
                     GSREAVDLRDGGDALAGLDVRLAVEHVSGIIAQRLAGLDAADQAAVDQALIALDGTPN
                     KARLGGNATIAVSMACLHAAAAAYGEPLWRHLAQGRPVRLPLPEIQIFGGGAHAGRRI
                     DIQDLMVMALSAGSFDEALGMTAEVYRAGGALMQERGLLQGVADEGGFWPAFDSNEEA
                     LDMLVRAIERAGYRPGDQIGISLDVAASEFGRDGRYRLALEARELDSLEMAELLMDWC
                     KRYPIVSIEDPLAEDDAAGMELFTAAMGDRIQIIGDDYLVTSAALVQKAAAERACNAV
                     LLKPNQAGTLTETHAALRAGQAAGFGTIVSARSGETEDVTIVHLAVGWDAGQLKVGSF
                     ARSERMA"
BASE COUNT          478 a         1208 c         1233 g          492 t
ORIGIN      
        1 ggcaccaggt acacgccgcc ggtgtccggc accgacgccg cgaccgcctc gacctcgtcg
       61 gcggagcgga tcaggcacag cccgtcgcgc agccactgca cggccgcgcc ggcgacgaag
      121 acgctgcctt cgagggcgaa ctcgcggcgg ccggcctgct gccaggccac ggtggtgagc
      181 agccggttgt gcgaatcgac cgagcgctcg ccggtgtgca tcagcatgaa acagccggtg
      241 ccgtaggtgt tcttgaccat gccgggggtc acgcagcgct gcccgaacaa cgccgcctgc
      301 tggtcgccgg cgatgccggc gatcggcagc gcccggccga acaaggcggc gtcggtttcc
      361 gccacggtgc cggtggacgc caccacctgc ggcagcattg cacgcggcac gcgcatcagc
      421 tgcagcagct tgtcgtccca atcgccggcg tgaatgttga acaacagcgt gcgcgaggcg
      481 ttgctcgcat cggtgaggtg ggcgccgctc agcttccaga ccagccaggt gtcgatcgtg
      541 ccgaaggcca gctcgccggc ctcggcgcgc gcgcgggcgc ccgggacatg atccaggatc
      601 cagcgcacct tggtgcccga gaagtacggg tcgagcagca ggccggtgcg gccggtgaac
      661 aacgcctcca gcccctgttg cttgagctgc tcgcacagcg cggcggtgcg gcggtcctgc
      721 cagacgatgg ccggatacac cgggcggccg gtagcgcgct cccacaccac tgtggtttcg
      781 cgctggttgg tgatgccgag ggcggcgatg tcctgcactg ttgcgcccgc gtgctcgatc
      841 gcctcgcgcg ccaccgcgag ctgcgtttgc cagatctcct cggggtcctg ctcgacccag
      901 cccggctgcg gatagctctg cgtggtctcg cgctgcgcca ggccgctcgg ctcgcctgcc
      961 cgatcgaaca gcagcgcgcg cgagctggtg gtgccctggt cgagcgcgag gacgtaacgc
     1021 atggcggttg tgctaaagtg cgggcgcttt tccacggccc cttgcaccgg ggctcggtca
     1081 gcatacaccc gttcgcgcaa gtcacatgcc agtgaaaaca accggcccgg ccgacggctt
     1141 cgccgccgcg ctgaaccgga tgggcttgct gcaacccggt gaaactccgc gcatccgccg
     1201 gctccccggc ggcgtgtcct cggacatcgt gcgcgtcgat ctcgcccgcg gtccggtttg
     1261 catcaagcgc gcgctcccca ggctcaaggt cgcggcagac tggcgcgcgc cggtggagcg
     1321 aaatcgctgg gaggtggcgt ggatgcgcgt agtcgcgcgc gtggagccgg acgcggtgcc
     1381 cgcggtgctg ggcgaggacg tcgatggcgg catgttcgcc atggcttacc tggacgaggc
     1441 gcagcacccg gtgtggaaag cgcggctgcg cgacggccac atcgacgcgg cgttcgccgc
     1501 cgaggtgggc aggcgcgtgg cgcacatcca cgcggcgacc gcgggcgacg cagccgtgca
     1561 cgagcgcttc gccaccgatc acatcttcca tccgatccgc atcgagccct acctggtggc
     1621 caccgggcgg gcgcatcccg acctcgcgcc gcggctcgac gcgctggcgc gcgacaccgc
     1681 tcacacccgc ctcgcgctgg tgcacggcga cgtcagcccg aagaacatcc tgtgcggccc
     1741 gcgcggaccc gtgttcctgg acgccgagtg cgcgtggtac ggcgaacccg ccttcgacct
     1801 ggccttctgt ctgaatcacc tgctgctcaa gtgcctgtgg cgcccgcgct ggagcgcgcg
     1861 ctatctcgag tgcttcgacg cgctggccgc agcgtacctc gagggcgtaa cctgggagcc
     1921 gcgcgcgcgg atcgagacgc gcaccgcgca tctgcttccc ggcctgttcc tcgcgcgcgt
     1981 ggacggcaag tcgccggtcg agtacgtcac gcaggactgg cagcgcagcg cggtgcgcga
     2041 agtcgcgcgc ggcttgctcg aatcgcccgt cgacaacgtc ggcgtgattc gcgatgtctg
     2101 ggaggcgcgc tgcgccggct ttccaggggg atgatgcaag tgtcgacaac tgccctttgt
     2161 ttttcccctc accccaaccc tctcccgcaa ggggagaggg ggtaccagac ctgatgctca
     2221 ccaccatcac cagcgtcaag ggccgccgcg tgtgggactc gcgcggccgg cccaccgtgg
     2281 aggccgaggt gcgcctggcc ggcggcgcga tcggccgcgc catcgccccg gcgggcgctt
     2341 cgcgcggctc gcgcgaagcc gtggacctgc gcgacggcgg cgacgcgctc gccgggctgg
     2401 acgtgcgcct ggcggtggag catgtatcgg gcatcatcgc ccagcgtctg gccggcctag
     2461 acgccgccga ccaggccgcg gtggaccagg cgctgatcgc gctggacggg acgcccaaca
     2521 aggcacggct cggcggcaat gcgaccatcg cggtgtcgat ggcgtgcctg catgcggccg
     2581 cggcggcata cggcgagccg ctgtggcgcc acctcgcgca gggccgtccg gtgcgcctgc
     2641 ccctgcccga gatccagatc ttcggcggcg gcgcgcacgc cggccgtcgc atcgacattc
     2701 aggacctgat ggttatggcg ctgtcggcgg gcagcttcga cgaggcgctg ggcatgaccg
     2761 ccgaggtgta ccgcgccggc ggcgcgctga tgcaggagcg cgggctgctg cagggggtgg
     2821 ccgacgaggg cggcttctgg ccggccttcg acagcaacga agaggcgctc gacatgctgg
     2881 tgcgcgcgat cgagcgcgcg ggctaccgcc cgggcgacca gatcggcatc tccctcgacg
     2941 tcgccgcctc cgagttcggg cgcgacggcc gctaccgcct ggcgctggag gcgcgcgagc
     3001 tcgacagcct ggagatggcc gagctgctga tggactggtg caagcgctat ccgatcgtct
     3061 cgatcgagga cccgctggcc gaggacgacg ccgcgggcat ggagttgttc accgcggcca
     3121 tgggcgaccg catccagatc atcggcgacg actacctggt caccagcgcc gcgctggtgc
     3181 agaaggcggc ggcggaacgc gcctgcaacg cggtgctgct caagcccaac caggcgggca
     3241 cgctcaccga aacgcacgcc gcgctgcgcg ccggccaggc ggcggggttc ggcaccatcg
     3301 tgtcggcgcg ctcgggcgaa accgaagacg tcaccatcgt gcacctggcg gtgggctggg
     3361 atgccggcca gctcaaggtg ggctcgttcg cccgctccga gcgcatggcc a
//
LOCUS       DASPGE010000227         2224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_763679, whole genome shotgun sequence.
ACCESSION   DASPGE010000227 DASPGE010000000
VERSION     DASPGE010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763679"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VD791_07275"
     CDS             <1..759
                     /locus_tag="VD791_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXV07812.1"
                     /translation="TRRVALEAELRGAVPRGELAVQYQPMARLADGKVVAHEALLRWQ
                     HPQHGLVAPGDFLQVAEDTGLILGIGDWVLGEACRWGTFIGIEHGVQVAVNLSAREFH
                     DARLIERVRRVLADSGLPPGLLALEVSEATVMHQPDVSASTLKKLRDAGVSLAIDDFG
                     TGPCSLPALCQLPVDRLKIDRTVVAGAPASPSAAALLGGIVGLAHAIGLAVVAEGVET
                     EAQREQLAALGCDAVQGFLVGRPVDADAASKDYV"
     gene            complement(756..1706)
                     /locus_tag="VD791_07280"
     CDS             complement(756..1706)
                     /locus_tag="VD791_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXV07813.1"
                     /translation="MRPLAALLLALAATLAQAQSFPTRPITFVVPFGAGGPADIYARF
                     LGQRMQASLGQFVVENRPGGGSVVGSNAVAQSAPDGYTLLVMSNTHTVNETLIPKKPF
                     ELMRDFVPVAPINYSDLLLVVHPSVGVSDVKGLIALAKSQPGKLNYASSGPGTPYHMA
                     GELFKSLAGIDVVHIPHKGSDGARLSILSGQVQLMFDAITTMSANARAGKVNALATSG
                     RARSAIMPEVPTVAEAGVPGYEATIWLGVVAPKGTPKEIVERLNAEITKVTSSAEVKE
                     AWAKQGASPLAMNPAQFEQYLIADIAKWRKVITDAGIKRD"
     gene            complement(1724..>2224)
                     /locus_tag="VD791_07285"
     CDS             complement(1724..>2224)
                     /locus_tag="VD791_07285"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV07814.1"
                     /translation="EQLDMALFVHPQGPSIGQERMGQYYLPFMVGYPSDSALAAARLI
                     LGGVLERLPRLRVCFAHGGGAFPATLGRLDHGHRVRPEAKKFIALPPSHYARRLYFDT
                     LTHDAKLLAFLLEKFGSERVMIGSDYPFDMGVEHPLAQLEGVPLNAADADNLTHKTAR
                     RFLRLS"
BASE COUNT          309 a          750 c          834 g          331 t
ORIGIN      
        1 acgcgccgcg tcgcgctgga agccgagctg cggggggcgg tgccgcgggg cgagctggcc
       61 gtgcagtacc agccgatggc gcgcctggcc gacggcaagg tcgtggcgca cgaggcgctg
      121 ctgcgctggc agcacccgca gcacgggctg gtggcgccgg gcgatttcct gcaggtggcg
      181 gaggacaccg gcctcatcct gggcatcggc gactgggtgc tcggcgaggc ctgccgctgg
      241 ggcacgttca tcggcatcga gcacggtgtg caggtggcag tcaacctctc ggcgcgcgag
      301 ttccacgatg cgcggctgat cgagcgggtg cggcgcgtgc tcgccgacag cggcctgccg
      361 ccggggctgc tggcgctcga agtgagcgaa gccaccgtca tgcaccagcc cgacgtgtcc
      421 gcgagcacgc tgaagaagct gcgcgacgcg ggcgtgtcgc tcgccatcga tgatttcggc
      481 accgggccgt gcagcctgcc ggcgctgtgc cagctgccgg tggaccggct gaagatcgac
      541 cgcacggtgg tggccggcgc ccccgcgtcg ccgagcgcgg ccgcgctgct cggcggcatc
      601 gtcggcctgg cgcacgccat cggcctcgcc gtggtcgccg agggcgtgga gaccgaggcg
      661 cagcgcgagc agctcgcggc gctcggctgc gacgccgtgc agggattcct ggtcggccgc
      721 ccggtcgacg ccgacgcggc ctccaaggac tacgtctagt cgcgcttgat gccggcgtcg
      781 gtgatcacct tgcgccactt ggcgatgtcg gcgatcaggt actgctcgaa ctgcgcggga
      841 ttcatggcca gcgggctcgc gccctgcttg gcccacgcct ccttcacctc cgcgctcgag
      901 gtcaccttgg tgatctcggc gttcaagcgc tccacgatct ccttcggcgt gcccttcggg
      961 gccaccacgc cgagccagat cgtcgcctcg tagcccggca cgccggcctc cgccacggtc
     1021 ggcacctccg gcatgatcgc cgagcgcgcg cggccgctgg tcgccagcgc attgaccttg
     1081 ccggcgcgcg cgttcgccga catcgtggtg atggcgtcga acatcaactg cacctggccg
     1141 gagaggatgg acaggcgcgc gccgtcgctg cccttgtgcg ggatgtgcac cacgtcgatg
     1201 ccggccagcg acttgaagag ctcgccggcc atgtggtacg gcgtgcccgg gccggaggag
     1261 gcgtagttga gcttgcccgg ctggctcttg gcgagggcga tcagtccctt cacgtcgctc
     1321 acgcccaccg aggggtgcac gaccagcagc aggtcggagt agttgatcgg cgccaccggc
     1381 acgaagtcgc gcatcagctc gaagggcttc ttcgggatga gcgtctcgtt gaccgtgtgc
     1441 gtgttggaca tgacgagcag cgtgtagccg tcgggcgccg actgggcgac ggcgttcgag
     1501 ccgaccaccg agccgccgcc ggggcggttc tccaccacga actggccgag cgaggcctgc
     1561 atgcgctggc cgaggaagcg cgcgtagatg tcggccgggc cgcccgcgcc gaacggcacg
     1621 acgaaggtga tcggccgggt ggggaaggac tgggcctgcg ccagcgtggc ggcgagcgcg
     1681 agcagcagtg cggcgagcgg tctcatctga gtctcctctt ctttcaggaa aggcgcagga
     1741 agcgccgggc ggtcttgtgg gtcaggttgt cggcgtcggc ggcgttcagg ggcacgccct
     1801 ccagctgcgc gagcgggtgc tcgacgccca tgtcgaaggg atagtcgctg ccgatcatga
     1861 cgcgttcgga gccgaacttc tccagcagga aggcgagcag cttcgcatcg tgcgtgagcg
     1921 tatcgaagta caggcggcgc gcatagtgcg acgggggcag cgcgatgaac ttcttcgcct
     1981 ccggacgcac gcggtggccg tgatccaggc ggccgagggt cgcggggaag gcgccgccgc
     2041 cgtgcgcgaa gcacacgcgc aggcgcggca gccgttccag caccccgccg aggatgagcc
     2101 gcgccgccgc gagcgccgag tcggaggggt agcccaccat gaacggcagg tagtactggc
     2161 ccatgcgctc ctgcccgatc gagggtccct gcgggtgcac gaacagcgcc atgtccagct
     2221 gctc
//
LOCUS       DASPGE010000228         6327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_60930, whole genome shotgun sequence.
ACCESSION   DASPGE010000228 DASPGE010000000
VERSION     DASPGE010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60930"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VD791_07290"
     CDS             complement(<1..1058)
                     /locus_tag="VD791_07290"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HXV07815.1"
                     /translation="MARSAGAALTYSSYLQLDTLLGCQRPESAKSGAPAHDELLFIVT
                     HQTYELWFKQVLHELDAVLATMRQPAVPERDLAQVLHWLERIVAIQRVLVQQIDVLET
                     MTPLDFLDFRDLLAPSSGFQSVQFRHLENRLGLDRKQRVGYNAMPYESALPPAVQRQL
                     RTLEQRPSLFALVEQWLARMPFLRFREYDFWQAYRDNVQSMLDKDRAAIERNTLLSDA
                     ARTIQLQELAKTRASFETVFDPARYETLVQSGERRLSYAAFLSALMINLYRDEPMFHI
                     PFRVLTALVAIDESLTLWRYRHALMVNRMIGSKIGTGGSSGQDYLKATVEAHRIFTDF
                     FNLSTYLVPRSMLPRLPD"
     gene            complement(1062..1913)
                     /locus_tag="VD791_07295"
     CDS             complement(1062..1913)
                     /locus_tag="VD791_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HXV07816.1"
                     /translation="MTAQMDPRLAAGNRRPLAQYRAQHFGWEVAERVGVVTLDRPERK
                     NPLTFDAYAELRGLFRRLYHANDVRVVVITGAGENFCSGGDVHEIIGPLTQLDMPGLL
                     TFTRMTGEVVKAMRGCPQPIVSAVDGVCAGAGAILAMASDIRFGTARSRTAFLFVRVG
                     LAGADMGACAILPRIVGHGRAAELLYTGRTLSGEEALQWGFFNRLCAPESVLSEAHTF
                     AEELARGPTFAHTMTKRCLHQEWSMGLDQAIEAEAQAQAICMQTQDFRRAYRAFAAKQ
                     KPVFEGD"
     gene            complement(1915..2454)
                     /locus_tag="VD791_07300"
     CDS             complement(1915..2454)
                     /locus_tag="VD791_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXV07817.1"
                     /translation="MDLEARVSDDHHQAVKLWLRLLACTNRIEADIRGRLRTEFGITL
                     ARFDLMAQLERSPAGLKMSELSKRMMVSGGNVTGLTDELEKEGLVAREDDPADRRAYT
                     VKLTPAGRALFGRMATVHERWVIELLAGLNGGEKTRVYRLLAKLKQHLAAPTPVDQTQ
                     PDRPQRVAAEQRNNGRRRN"
     gene            complement(2526..4805)
                     /locus_tag="VD791_07305"
     CDS             complement(2526..4805)
                     /locus_tag="VD791_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HXV07818.1"
                     /translation="MKVVCIGGGPAGLYFSILMKQADPRHDILVVERNRPDDTFGWGV
                     VFSDATLSHFQQADPRSHAQILESFHHWDNIDVFFKGRRITSGGHGFCGIGRKQLLNI
                     LQARAAELGVEQRFEQEVTDPDQFADADLIVASDGVNSVTRRKHAQVFQPDIDVRKCR
                     YIWLGTHRRLDAFTFAFQETEWGWFTLHAYRFDADTSTFIVETPEENWLAAGLDRMDQ
                     AQSIAFCERLFADLLEGRSLMSNAGHLRGSAWLKFNRVLCKQWQHRNIVLIGDAAHTA
                     HFSIGSGTKLAMEDAISLARALQREPDVASALPLYQQEREVEALRLQSAARNRMEWFE
                     NVARYAHLDAIQFSYTLLTGSQRLGHENLRLRDSRYVERVEKWMAYRAGLAVQPVPPM
                     FTPFRLRGLTLKNRVVVSPMAMYSARDGVPGDFYLVHLGARALGGAGLVFTEMTCVSP
                     EGRISPGCAGLWNEAQARAWRRIVDFVHSDSSAAIGLQLGHSGPKGSTQLGWEQIDEP
                     LPSGNWQLIAPSPVAYGPQNQVPRAMTRADMDLVLRQFVDATRRGVECRFDMLELHCA
                     HGYLLSSFICPLTNLRTDAYGGALENRMRFPLEVFRAMRALWPEDRPMSVRISAHDWA
                     PGGNTPDDAVEIARMFKAAGADMIDVSSGQTTRLAKPVYGRMYQTPFSDRIRNEVGIG
                     TIAVGNIFEADHVNSIVAAGRADLCALARPHLADAAWTLQAAAELGFHDVEWPRQYLT
                     GKAQLERNIARAAQLAIQA"
     gene            4957..5241
                     /locus_tag="VD791_07310"
     CDS             4957..5241
                     /locus_tag="VD791_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HXV07819.1"
                     /translation="MVTAIVLLTVSRGRVSEVAEQLAAQEGISEVYSVAGRFDLVAMV
                     RVKANEDLADVVTKKLQRVDGIQTTETLIAFRAYSRVDLEGVFSLGFERK"
     gene            complement(5263..5580)
                     /locus_tag="VD791_07315"
     CDS             complement(5263..5580)
                     /locus_tag="VD791_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HXV07820.1"
                     /translation="MGPLTPALLGEQLEVALDELARTTGLSVEEVVELVEYGVFEPAG
                     GTRVQWRFSARCIALGRRAARLRADFELDLSGLALVAALLERVDDLEAEMARLRAQLL
                     TER"
     gene            complement(5596..>6327)
                     /locus_tag="VD791_07320"
     CDS             complement(5596..>6327)
                     /locus_tag="VD791_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HXV07821.1"
                     /translation="FRPPPDWSERFGGGARGGFGGFEDLGDLDLFDLLAGLGGRGGFG
                     RRAGSRDMRMSGQDVEVTLYAGIDDLMRGTEVEIPLSFTEIGAEGRPQRVTRTTKVRI
                     PKGATDGQRLRVPGKGGPGIGGGPPGDLYINISLRPHELFRVSGHDVYLEVPITPWEA
                     ALGATVEVPTPQGKVSLKVPPGSRAGQKLRVRGKGLPRPKSDEHGDFYAVLQIVTPSV
                     LSEREKALYRDLAQASTFDPRAHFE"
BASE COUNT          963 a         2216 c         2143 g         1005 t
ORIGIN      
        1 tcgtcgggca gccgcggcag catcgagcgc ggcaccagat aggtggacag gttgaagaaa
       61 tcggtgaaga tgcggtgcgc ctcgacggtc gccttcaggt aatcctgccc ggaggagccg
      121 ccggtgccga tcttgctgcc gatcatgcgg ttgaccatca gcgcatgccg gtagcgccac
      181 aaggtcaagc tctcgtcgat cgccaccagc gcggtgagca cccggaacgg gatgtggaac
      241 atcggctcgt cccggtacag gttgatcatc agcgccgaca ggaacgccgc atacgacagc
      301 cgccgctcac cgctttgcac cagagtctcg tagcgcgccg ggtcgaacac ggtttcgaag
      361 ctcgcccggg tcttggccag ctcctgcagt tggatcgtcc tggccgcgtc gctgagcagc
      421 gtgttgcgct cgatcgcggc gcggtccttg tccagcatcg actgcacgtt gtcgcggtag
      481 gcctgccaga agtcgtactc gcggaagcgc aggaacggca tgcgcgccag ccattgctcc
      541 accagcgcga acaggctcgg gcgctgctcg agcgtgcgca gctggcgctg gacggcgggc
      601 ggcaacgccg attcgtaggg catggcgttg tagccgacgc gctgcttgcg atcgaggccc
      661 agccggttct cgaggtggcg gaactgaaca ctctggaagc cggaagacgg cgccaggaga
      721 tcgcggaaat ccaggaagtc gagcggcgtc atcgtttcca gcacgtcgat ctgctgcacc
      781 agcacgcgct ggatcgcgac gatgcgctcg agccagtgca ggacctgtgc gagatcacgc
      841 tccggcaccg ccggctggcg catcgtggcg agcacggcat ccagctcgtg caggacctgc
      901 ttgaaccaca gctcgtaggt ctggtgcgtg acgatgaaca gcagctcgtc gtgcgccggc
      961 gcgccgctct tcgcgctctc cggccgctgg caaccgagca gggtgtcgag ctgcagatag
     1021 ctgctgtagg tcagcgcggc gccggcggat cgcgccatgg ctcagtcgcc ctcgaacacc
     1081 ggcttctgct tggcggcgaa ggcccgatac gcgcgccgga aatcctgcgt ctgcatgcag
     1141 atcgcctggg cctgggcttc ggcctcgatg gcctggtcca gccccatgct ccactcctga
     1201 tgcaggcacc tcttggtcat ggtgtgggcg aaggttggac cgcgcgccag ctcctcggcg
     1261 aaggtgtgcg cctcggacag gaccgattcg ggtgcgcaca ggcggttgaa gaagccccac
     1321 tgcagcgctt cctcgccgct cagtgtacgg cccgtgtaca gcagctcggc cgcccggccg
     1381 tgcccgacga tgcgcggcag gatggcgcat gcgcccatgt ccgcgccggc caggccgacg
     1441 cgcacgaaca ggaacgccgt ccggctgcgc gcggtgccga atcggatgtc ggacgccatc
     1501 gccaggatcg caccggcccc ggcgcacacg ccgtccaccg ccgacacgat cggctgcggg
     1561 cagccccgca tcgccttgac cacctcgccg gtcatgcgcg tgaaagtcag cagcccgggc
     1621 atgtcgagct gggtcagcgg cccgatgatc tcgtggacgt ccccgcccga gcagaaattc
     1681 tcacccgccc cggtgatgac caccacacgc acgtcgttgg cgtggtagag ccggcggaac
     1741 aggccgcgca gctcggcata cgcgtcgaag gtgagcgggt tcttgcgctc cggccggtcg
     1801 agagtgacca cgcccacccg ctcggccacc tcccagccga aatgctgcgc ccggtactgc
     1861 gccagcggcc gccggttacc ggccgccagc cgtggatcca tctgcgccgt catgtcagtt
     1921 cctccgcctt ccgttgttgc gctgctctgc agcgacccgt tggggccgat cgggttgggt
     1981 ctgatccacc ggggtgggcg cagcgaggtg ctgcttgagc ttggcgagca gccgatacac
     2041 ccgggtcttc tccccgccgt tgagcccggc cagcagctcg atcacccagc gctcgtgcac
     2101 cgtcgccatc ctgccgaaca gcgcacggcc ggcgggcgtg agcttcaccg tgtaggcacg
     2161 ccggtcggcg ggatcgtcct cgcgtgcaac caggccctcc ttctccagct cgtcggtgag
     2221 cccggtgacg ttgccgccgc tcaccatcat gcgcttggac agctcgctca tcttcaggcc
     2281 ggcggggctg cgctcgagct gcgccatcag atcgaagcgc gccagcgtga tgccgaactc
     2341 ggtgcgcagc cgcccgcgga tatcggcctc gatgcggttg gtgcaagcga gcaggcgcag
     2401 ccagagcttg accgcctggt ggtggtcgtc gctgacccgc gcctccaggt ccacggtctt
     2461 cgcgcgtacg ccgctccgaa tcgctggcct cacgtcgctt ccccgtccat tagcggcccg
     2521 agcgctcacg cctggatggc gagttgcgcg gcgcgggcga tgttgcgctc gagctgcgcc
     2581 ttgccggtga ggtattgcct gggccactcc acgtcgtgga agccgagctc ggccgcggcc
     2641 tgcagggtcc aggccgcgtc ggcgagatgc ggccgcgcga gcgcgcacag atcggcgcgc
     2701 ccggcggcga cgatgctgtt gacgtggtcg gcctcgaaga tgttgccgac cgcgatggtg
     2761 ccgatgccca cctcgttgcg gatgcgatcc gagaacggcg tctggtacat gcgcccgtac
     2821 accggcttgg ccagccgcgt ggtctggccg gaggacacgt cgatcatgtc ggcgcccgcc
     2881 gccttgaaca tacgcgcgat ctcgaccgcg tcgtcgggcg tgttgccgcc cggggcccag
     2941 tcgtgcgcgg agatgcgcac cgacatcggt ctgtcctcgg gccacagcgc gcgcatcgcg
     3001 cggaacacct cgagcgggaa gcgcatgcgg ttttccagcg cgccgccgta cgcgtcggtg
     3061 cgcagattgg tgagcgggca gatgaagctc gacagcaggt agccgtgcgc gcaatgcagc
     3121 tccagcatgt cgaagcggca ctcgacccca cgccgcgtgg cgtccacgaa ctgccgcagc
     3181 accaggtcca tgtcggcgcg ggtcatggcg cgcggcacct ggttctgcgg cccgtaggcc
     3241 accggcgagg gcgcgatcag ctgccagttg cccgacggca gcggttcgtc gatctgctcc
     3301 cagccgagct gggtcgagcc cttcggcccg gagtgcccga gctgcagccc gatcgccgcg
     3361 ctggaatcgc tgtgcacgaa gtccacgatg cgcctccagg cccgggcctg cgcctcgttc
     3421 cacaacccgg cgcagcccgg gctgatgcgc ccttcgggcg acacgcaggt catctccgtg
     3481 aacaccaggc ctgctccgcc gagcgctcgc gcccccagat gcaccaggta gaaatcgccg
     3541 ggcaccccgt cgcgcgccga gtacatcgcc atcggcgaca ccaccacccg gttcttcagc
     3601 gtcagcccgc gcagccggaa cggcgtgaac atgggcggca ccggctgcac cgcgagcccg
     3661 gcgcggtaag ccatccactt ctccacccgc tccacgtagc ggctgtcgcg caggcgcagg
     3721 ttctcgtggc ccaggcgctg gctgccggtg agcagcgtgt aggagaactg gatcgcgtcc
     3781 agatgcgcat agcgcgcgac gttctcgaac cactccatgc ggttgcgcgc ggcgctctgc
     3841 agccgcagcg cctccacctc gcgctcctgc tggtacagcg gcagcgcgct ggccacgtcc
     3901 ggttcgcgct gcagcgccct ggcgagcgaa atggcatcct ccatcgccag cttggtgccc
     3961 gagccgatcg agaagtgcgc ggtgtgcgcg gcatccccga tcagcacgat gttgcgatgc
     4021 tgccactgct tgcacagcac gcgattgaac ttcaaccagg ccgacccgcg caggtgcccg
     4081 gcgttgctca tcagcgagcg cccctccagc aggtcggcga acagccgctc gcagaaggcg
     4141 atcgactgcg cctgatccat gcggtccagc ccggccgcca gccagttttc ctccggggtc
     4201 tcgacgatga aggtgctggt gtcggcgtcg aagcggtacg cgtgcagcgt gaaccagccc
     4261 cattcggtct cctggaacgc gaaggtgaac gcgtccagcc gccggtgcgt gccgagccag
     4321 atgtagcggc acttgcgcac gtcgatgtcg ggctggaaca cctgcgcgtg cttgcgccgc
     4381 gtcaccgagt tgacgccgtc ggaggcgacg atcagatcgg cgtccgcgaa ctgatccgga
     4441 tcggtgacct cctgctcgaa gcgctgctcc acgcccagct cggcggcgcg cgcctgcagg
     4501 atgttgagca gttgctttcg gccgatgccg cagaagccgt ggccgcccga ggtgatgcgg
     4561 cggcccttga agaacacgtc gatgttgtcc cagtggtgga agctctccag gatctgggcg
     4621 tggctgcggg gatcggcctg ctggaagtgg gacagggtgg cgtccgagaa caccacgccc
     4681 cagccgaagg tgtcgtcggg gcggttgcgc tccaccacca ggatgtcgtg gcgcgggtcg
     4741 gcctgcttca tcaggatcga gaagtacagc cccgccggtc cgccgccgat gcacacgact
     4801 ttcatgcgcc gctcccggtt gcttcgatcg tgaatttata gagtataaat aattgatctg
     4861 tcaagcataa gcgcgctctt ggcacgactc ggccggttgt cgccgtcgtc cactggtgct
     4921 accttgcctg ccgatccagc gacaggaggc gaggcgatgg ttacggcaat cgttctgctg
     4981 acggtgtccc gcgggcgggt gagcgaagtg gccgagcagc tcgccgccca ggagggcatc
     5041 agcgaggtct attcggtggc gggtcgcttc gacctggtgg cgatggtgcg ggtgaaagcc
     5101 aacgaggacc tggccgacgt ggtgaccaag aagctgcagc gcgtcgacgg catccagacc
     5161 accgagacgc tgatcgcgtt ccgcgcctat tcccgggtgg acctggaagg cgtgttctcc
     5221 ctcggcttcg agcgcaagta gccctgcgcg ggcgccgagc gcctagcgct cggtcagaag
     5281 ctgcgcgcgc aaccgcgcca tctccgcttc cagatcgtcc acgcgctcga gcagcgccgc
     5341 caccagggcc aggccggaga ggtcgagctc gaaatcggcg cgcagccgcg cggcgcggcg
     5401 ccccagggcg atgcagcgcg cggagaaccg ccactgcacc cgcgtgccgc cggccggctc
     5461 gaacaccccg tattcgacca gctcgaccac ctcctcaacg ctcaggccgg tggtgcgggc
     5521 gagctcgtcg agcgcgactt cgagctgctc gcccagcagg gccggagtca gcggtcccat
     5581 ggccatggca tccccctatt cgaagtgcgc gcgcgggtcg aaggtcgacg cttgcgccag
     5641 gtcgcggtac agcgccttct cgcgctcgct caggaccgac ggcgtcacga tctgcagcac
     5701 ggcatagaag tcgccgtgct cgtcgctctt cggccgcggc agacccttgc cgcgcacgcg
     5761 cagcttctgg ccggcacgcg agccgggcgg gaccttcagg ctcactttgc cctgcggggt
     5821 cggcacctcg acggtggccc ccagcgccgc ctcccagggg gtgatcggaa cctccagata
     5881 gacgtcgtgg ccgctgacgc gaaacagctc gtgcggccgc aggctgatgt tgatgtacag
     5941 gtcgccgggc ggcccgccgc cgatgcccgg gccgcctttg cccggcaccc gcagccgttg
     6001 cccgtcggta gcgcccttgg ggatgcgtac cttggtagtc cgagtcaccc gctgtgggcg
     6061 gccctccgcg ccgatctcgg tgaaagacag cgggatctca acttcggtgc cgcgcatcag
     6121 gtcgtcgatc cccgcgtaca gggtgacttc cacgtcctgc ccggacatcc gcatgtcgcg
     6181 actgcccgcg cgccggccga acccgccgcg tccgcccagg cccgccagca ggtcgaacag
     6241 gtcgagatcg ccgagatctt cgaagccacc gaagccgccg cgcgcaccgc cgccgaagcg
     6301 ctcgctccaa tccgggggcg gacggaa
//
LOCUS       DASPGE010000229         2379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_997590, whole genome shotgun sequence.
ACCESSION   DASPGE010000229 DASPGE010000000
VERSION     DASPGE010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997590"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VD791_07325"
     CDS             <1..215
                     /locus_tag="VD791_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV07822.1"
                     /translation="IRFEIVYGVSANRRSWYDNSNAERLGYRPHDDSEPYAAEILARH
                     VDAADPRVELYQGGTFVATESNVKPT"
     gene            complement(343..540)
                     /locus_tag="VD791_07330"
     CDS             complement(343..540)
                     /locus_tag="VD791_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07823.1"
                     /translation="MTTPIKLLTGLLIGALISTSALAGNRIDVRMVQGAQQAKFSLGD
                     SKCVLVGDVIKCIPAITTASN"
     gene            875..>2379
                     /locus_tag="VD791_07335"
     CDS             875..>2379
                     /locus_tag="VD791_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXV07824.1"
                     /translation="MAIATAAEARTRFSLSSLEDKYTAAAGRYYMTGVQALVRLCINQ
                     RRRDLALHRNTAGFVSGYRGSPLGPLDKEFWKAAGPLGEAHVRFQPGVNEELAATAVI
                     GTQQLHLFPGARYDGVFSLWYGKAPGVDRCADAFRHGNFAGTGRHGGVLLIAGDDHGA
                     YSSSLPNQSDLLFMHCFIPLVHPAGVQEFLDLGVHGWAMSRYSGCWIGFKALADTVES
                     SAVVDVDPLRVRVHIPEDFALPEGGLNIRLPETRFEQEARLMDYRLDAVRAYVRANRL
                     NRVTQDSPFPRIGIVGVGKAWLDVLKALEDLGVDSAQAAQLGVRLFKVAVPWPLERES
                     ALRFARGLEEIVVVEEKRPLVEAQLKELLYALPEHARPRIIGKDIAPADRDFDRRLLL
                     PEKLDFSPAEVALLLASRLGAQGGAQRGSERMRARVAQIEAARAAAHADAVDLQRLPW
                     YCSGCPHNTSTKVPEGSFALAGIGCHWMANWITAETTRTTVQMGGEGATWMG"
BASE COUNT          369 a          826 c          826 g          358 t
ORIGIN      
        1 cgatccgctt cgagatcgtg tacggggtgt ccgccaaccg ccgctcctgg tacgacaact
       61 ccaacgccga gcggctgggc taccgtccgc acgacgacag cgagccgtac gcggcggaga
      121 tcctggcccg gcacgtggac gcggccgacc cgcgcgtgga gctgtaccag ggcggaactt
      181 tcgtggcgac ggaatcgaac gtcaagccca cctgattgcc ccctcacccc ggccctctcc
      241 cgcaagggga gagggagcgt catgcctgtc ggcacaaata aagcggcccg cgcgaggcgg
      301 gccgaaaggg ggaggagacg acgaagaaac ggttcgggca gatcagttcg aggcggtggt
      361 gatcgcgggt atgcacttga tcacgtcgcc gaccagcacg cacttggaat cgcccaggct
      421 gaacttggct tgctgcgcgc cctggaccat gcgcacatcg atgcggttgc cggccagggc
      481 gctggtggaa atgagcgcgc cgatcagcag gccggtcagc agtttgatgg gggtggtcat
      541 ggtcgggtcc tcgcttgcgt ttgctgttgt gaccactgta cgcggacaag cccctcgacc
      601 cgcatgaact attgcgggtc gccgaggtga actattcaca ccccggctcc ggacccggcc
      661 gagggccttc ctgcaccgca acatacgaac cctgaatccc gggccgcgcg ccgcgcacgg
      721 aatatgctcg gcacccgcgg tgaaggcgca gggctagtcc tgcgcacagc atccggtcgg
      781 aaccgggcat ccgcgcgatg ccccgcggtg cacggcccac aaagtcgcgc ccaagccccg
      841 accggcccca cgacacgatt tggggagcac cgggatggcc atcgctaccg ccgcagaagc
      901 gcgcacgcgc ttttcgcttt cctccctgga ggacaagtac accgccgccg ccggccgcta
      961 ttacatgacc ggcgtccagg ccttggtccg gttgtgcatc aatcagcgcc ggcgcgatct
     1021 cgcgttgcac cgcaacaccg caggcttcgt gtcgggctat cgcggctcgc cgctcggccc
     1081 cctggacaag gagttctgga aggccgccgg cccgctgggc gaggcgcacg tgcgcttcca
     1141 gccgggggtg aacgaggagc tggcggccac cgcggtgatc ggcacgcagc agctgcactt
     1201 gtttccgggc gcgcgctacg acggcgtgtt ctcgctgtgg tacggcaagg cgcccggcgt
     1261 ggaccgctgc gcggacgcgt tccggcacgg caacttcgcc ggcaccgggc gccatggcgg
     1321 cgtgctgctg atcgccggcg acgaccacgg cgcctactcc tcgtcgctgc cgaaccagag
     1381 cgatctgctg ttcatgcact gcttcatccc gctggtgcac ccggccggag tgcaggagtt
     1441 cctcgacctg ggcgtccacg gctgggcgat gtcgcgctac tcgggctgct ggatcggttt
     1501 caaggcgctc gccgacacgg tggaaagctc ggccgtggtg gacgtggatc cgctgcgggt
     1561 gcgggtgcac attcccgagg acttcgcgct gcccgaaggc ggcctcaaca ttcggctccc
     1621 cgagacgcgc ttcgagcagg aggcgcggct catggattac cggctcgacg cggtgcgcgc
     1681 ctacgtgcgc gcaaaccgcc tgaaccgcgt cacccaggac tcgcccttcc cgcgcatcgg
     1741 catcgtcggt gtgggcaagg cctggctcga tgtgctgaag gcgctcgagg acctcggcgt
     1801 cgacagcgcc caggccgcgc agctcggcgt gcgcctgttc aaggtggcgg tgccctggcc
     1861 gctcgagcgc gagagtgccc tgcgcttcgc gcgcggcctg gaagagatcg tcgtggtcga
     1921 ggagaagcgt cctctggtcg aggcgcagct caaggagctg ctgtacgcgc tgcccgagca
     1981 cgcgcgcccg cgcatcatcg gcaaggacat cgcgccggcg gaccgcgact tcgaccgccg
     2041 gctgctgctg ccggaaaagc tcgacttctc gcccgccgag gtggcgttgc tgctcgcatc
     2101 gcgcctgggc gcccaaggcg gcgcccagcg cggttcggag cgcatgcgcg cgcgcgtggc
     2161 gcagatcgag gccgcgcgcg ccgcggcgca cgccgacgcg gtggacctgc aacggctgcc
     2221 ctggtactgc tcgggctgcc cgcacaacac ctcgaccaag gtgcccgagg gcagcttcgc
     2281 cctggccggc atcggctgcc actggatggc caactggatc accgccgaaa ccacccgcac
     2341 cacggtgcaa atgggcggtg agggcgcgac ctggatggg
//
LOCUS       DASPGE010000230         3768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_178986, whole genome shotgun sequence.
ACCESSION   DASPGE010000230 DASPGE010000000
VERSION     DASPGE010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178986"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1047
                     /locus_tag="VD791_07340"
     CDS             112..1047
                     /locus_tag="VD791_07340"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005091.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HXV07825.1"
                     /translation="MKIAVVGAGGVGGYFGARLAASGQDVWFIARGAHLEAMRREGLR
                     VLSANGDVLVKPVQATGRPSDVGTADLVMIAVKLWSTGEALRDARPMVGPDTAVVSFQ
                     NGVVAVDQVAEAYGRGRTLGGVAAIAAVIDKPGVIRHTGTMATLQFGELDGAMSPRIQ
                     ALQQACEKAGIQGRATDAIQKAIWEKYVFLVGASAMTALTRLPLGSVREDPDTRALFA
                     QIMAEAAALGRAKGVALDADLVDKLLGRLDGLPREMVASMLGDLERGNRLELPWLSGG
                     VAAMGKEAGVATPANQFVYAALKLYVDGRPADARK"
     gene            1155..2249
                     /locus_tag="VD791_07345"
     CDS             1155..2249
                     /locus_tag="VD791_07345"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXV07826.1"
                     /translation="MTASLTIRNIRARPVLVPIKRPVYSASGTIPSAPLILIDLETEE
                     GVAGRSYVCAYMASMLKPIVACLEYARDALKGDPLAPVDNDQKLRKRLTLFDTPGILG
                     IALSGVDMCTWDALARAQGVPLVKLLGGAVKPVRAYNSCGLWIQPVAKLADEAEALVA
                     EGGFSAIKLRLGRDDPAEDLAAVRSVLQRVGARITVMCDFNQRLTVNEAILRGRMLDD
                     EGLYWIEEPVRHDNYAGYAQLCAELRTPVQTGENLLNTFEMVQAIEAQSLDYVMPDVQ
                     RIGGVSGWLRAAALAHAYGIEMSSHLFPEFSTHLLAVTPTCHWLEYMDWATPVVTEPY
                     RIEDGHALIPDRPGAGIEWNEDAVKRYAVA"
     gene            2523..2801
                     /locus_tag="VD791_07350"
     CDS             2523..2801
                     /locus_tag="VD791_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07827.1"
                     /translation="MTRLAWAGVAALSLTFAASAALAQEKDERAGTCSDAKLQMEYFC
                     DASRAANDTMVATGTACTNAKKNVAAACEGKTEADKAYKFEEKGEKGQ"
     gene            2904..>3768
                     /locus_tag="VD791_07355"
     CDS             2904..>3768
                     /locus_tag="VD791_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07828.1"
                     /translation="MSKMQRQIGVHMSDEEYAQCDPAETAFQSPVPTQMVSNGEYNPM
                     PQTEQQAKVEALIKELADIQAKRHGIDRRRFLQTSAGMATAFLAMNKVFGPVFDVTEA
                     EAADIQMSDYRRKQLENQFIFDDQTHFVRDDFAQEGLLGLTKWAVGAKVNPRINDVPL
                     TLSRYKMDNYLKEIFLDSDTKISLLSGAPFDDPAWWLLSNDAIQNACRAVNKMAGGTR
                     MLGHTIITPKYPNWMDEVDRGIATLHPVSWKAYTIGDPLGPSKYSWRLDDEKLMYPFY
                     EKAVKSGINTIC"
BASE COUNT          697 a         1231 c         1314 g          526 t
ORIGIN      
        1 gacgattcat gaacgccccg cggaaaagtg gcggaagggt agcacaggcc gctgcgcggc
       61 acgggcgaag cggctaccat ctgccccgat taacggcggg gaggagaccg aatgaagatc
      121 gcggtggtcg gcgccggcgg cgtcgggggc tacttcggcg cgcgcctggc cgcgagcggc
      181 caggacgtct ggttcatcgc gcgcggcgcg cacctggaag ccatgcgccg cgaagggctg
      241 cgggtgctca gcgcgaacgg cgacgtgctg gtcaagccgg tgcaggctac cggccggccg
      301 tccgacgtcg ggaccgccga cctggtgatg atcgctgtca agctgtggtc gaccggggag
      361 gcgctgcgcg acgcgcggcc catggtcggt cccgacaccg cagtggtgtc gttccagaac
      421 ggcgtggtcg cggtggacca agtcgccgag gcgtacggcc gcggccgcac gctgggtggt
      481 gtcgccgcca tcgccgcggt gatcgacaag cccggtgtga tccgccacac cggcaccatg
      541 gcgacgctgc aattcggcga gctcgacggc gccatgagcc cacgcatcca ggcgctgcag
      601 caggcgtgcg agaaggccgg catccagggc cgcgccaccg acgcgatcca gaaggcgatc
      661 tgggaaaaat acgtgttcct ggtcggcgcc agcgcgatga cggcgctcac tcgcctgccg
      721 ctggggtcgg tgcgcgagga cccggacacg cgcgcgctgt tcgcgcagat catggcggag
      781 gccgcggcgc tgggccgcgc caagggcgta gcgctcgacg ccgatctggt ggacaagctg
      841 ctcggccggc tggacggcct gccgcgcgag atggtggcgt ccatgctggg cgacctggag
      901 cgcggcaacc gcctggagct gccctggctg tccggcggcg tcgcggcgat gggcaaggaa
      961 gccggtgtgg ccacccccgc gaaccagttc gtgtacgcgg cgctcaagct ctacgtcgac
     1021 ggccgtcccg ccgacgcgcg gaagtaagtc tctccaacac agaggacaca gagggacatc
     1081 gaggacacag agggacaacg gggaaagcaa gagcgaacga atgccgggcg atcaacctta
     1141 gacatggaga atccgtgact gcctcactca ccatccgcaa catccgcgcg cgtccggtgc
     1201 tggtgccgat caagcgcccg gtgtactcgg ccagcggcac gataccgagc gcaccgctga
     1261 tcctgatcga tctggagacc gaggaaggcg tcgccgggcg cagctacgtg tgcgcctaca
     1321 tggcgagcat gctcaaaccg atcgtcgcct gcctggagta cgcgcgcgac gcgctcaagg
     1381 gcgacccgct ggcgccggtg gacaacgacc agaaactgcg caagcgcctg accctgttcg
     1441 acacgcccgg catcctgggg atcgccttgt cgggcgtgga catgtgcacc tgggatgcgc
     1501 tggcgcgcgc ccagggcgtg cccctggtga agctgctggg cggcgctgtg aagccggtgc
     1561 gcgcctacaa cagctgcggg ctgtggatcc agccggtcgc caagctcgcc gacgaggccg
     1621 aagcgctggt cgccgaaggc ggcttcagcg cgatcaagct gcgcctcgga cgcgacgatc
     1681 cggccgagga cctcgccgcc gtgcgcagcg tcctgcagcg cgtgggcgcg cgcatcaccg
     1741 tgatgtgcga tttcaaccag cgcctgacgg tgaacgaggc gatcctgcgc gggcgcatgc
     1801 tggacgacga ggggctgtac tggatcgagg agccggtgcg tcacgacaac tacgccggct
     1861 acgcgcagct ctgcgcggag ctgcgcacgc cggtccagac cggcgagaac ctgctcaaca
     1921 ccttcgagat ggtgcaggcg atcgaggcgc agtcgctgga ctacgtcatg ccggacgtgc
     1981 agcgcatcgg cggggtgagc ggctggctgc gcgcggccgc cctggcccat gcctacggca
     2041 tcgagatgtc gagccacctg ttccccgagt tcagcacgca cctgctcgcg gtgacgccga
     2101 cctgccattg gctcgagtac atggactggg ccacgcccgt cgtcaccgag ccgtaccgga
     2161 tcgaggacgg ccatgcgctg atccccgacc gcccgggcgc ggggatcgag tggaacgagg
     2221 atgcggtgaa gcgctatgcg gtcgcgtaag gggggacggt tgaaggcgcg ccccgagcaa
     2281 tccggagacg agggcaggcg gcgctctccg cctggccctc gtgggtgtgg gactggcggc
     2341 gcctatgccg cgctgcaaaa tcgaattcca ttgacgccag gcctggcgca gcagtagatt
     2401 ctgtgtcccc ggggagggcg gcgcgctgcg cgcgcacagt gctccccgcg aataaccata
     2461 tccacgacgt agtgccccgg caggtgctca ctggccgagg ccaatgacct gggaggaagc
     2521 cgatgacacg tctcgcgtgg gcgggagtcg ccgccctttc cctcacgttc gccgcctccg
     2581 cagccctggc tcaggagaag gacgagcgcg ccggcacctg ctcggacgcg aagctgcaga
     2641 tggagtactt ctgcgacgcc agcagggccg ctaacgacac catggttgcc accgggaccg
     2701 cctgcacgaa cgccaagaag aacgtggccg cggcctgcga aggcaagacc gaggccgaca
     2761 aggcgtacaa gttcgaagaa aaaggagaaa agggccagta atttcggttt acgccctcga
     2821 cgcaccagca ggggtactcc ggcggggcgc ggacatgaca gggagtacgc agcgttgaca
     2881 acgtttctaa cggaggggct gctatgagca agatgcaaag acagatcggc gtgcacatga
     2941 gcgacgagga gtacgctcag tgcgatcccg ccgaaaccgc attccagtcg ccggtgccca
     3001 cacagatggt gtccaacggc gagtacaacc cgatgcccca gaccgagcag caggccaagg
     3061 tcgaggctct gatcaaggag ctggccgaca tccaggccaa gcggcacggc atcgaccgcc
     3121 gccgcttcct gcagaccagc gccggcatgg ccaccgcctt cctggcgatg aacaaggtgt
     3181 tcgggccggt gttcgacgtc accgaggccg aggccgccga catccagatg tcggactatc
     3241 ggcgcaagca gctcgagaac cagttcatct tcgacgacca gacccacttc gtacgcgacg
     3301 acttcgccca ggaaggcctg ctcggcctga ccaagtgggc ggtgggggcg aaggtcaatc
     3361 cgcgcatcaa cgatgtgccg ttgacgctgt cgcgctacaa gatggacaac tacctgaagg
     3421 agatcttcct cgacagcgac accaagatct ccctgctgtc gggcgcgccc ttcgacgatc
     3481 cggcctggtg gctgctgtcc aacgacgcca tccagaacgc ctgtcgcgcg gtgaacaaga
     3541 tggccggcgg cacgcgcatg ctggggcaca cgatcatcac gcccaagtat ccgaactgga
     3601 tggacgaggt cgaccgcggc atcgcaacgc tgcacccggt gagctggaag gcctacacca
     3661 tcggcgatcc gctcgggccg tcgaagtact cctggcgcct ggacgacgag aagctgatgt
     3721 acccgttcta cgagaaggcg gtcaagtccg gcatcaacac catctgca
//
LOCUS       DASPGE010000231         6393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1116428, whole genome shotgun sequence.
ACCESSION   DASPGE010000231 DASPGE010000000
VERSION     DASPGE010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116428"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..952
                     /locus_tag="VD791_07360"
     CDS             128..952
                     /locus_tag="VD791_07360"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HXV07829.1"
                     /translation="MNGKLTVSMPRVLPTRARNSSRRGIDAWFGLLICADASAHAGSA
                     QSWWSFDPWVWGGFALLLLLRPRRPASFLLAGAALFLALIWPLDVLAETSFAAHMAQH
                     MLLIGVAAPLLVLSRPSLGRVKAISLGGKRRVARLLRGWSTLSRPPTAFALHAAAVWI
                     GHWPRVIEAMILQRWVHILGHAVLLATALVFWWSLRSRGRAGAGNAALWTLATMVHTG
                     MLGALLAFAPRALYPAYSLSDQQLAGLIMWVPGALCYLAAGLVFAAAWLGDERRPA"
     gene            952..1893
                     /gene="coxB"
                     /locus_tag="VD791_07365"
     CDS             952..1893
                     /gene="coxB"
                     /locus_tag="VD791_07365"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HXV07830.1"
                     /translation="MRLLEGVQSALHPQGPAAAVVAEMFWVMCAGAAIILVAVTALAA
                     YAVFAPRKDAAILSGSSLIVAGGIVFPVLTLSALLLYSFVRAGSIAPAAPPPAVRIEV
                     VGEQWWWRVHYLDVGGRSDFVTANEIRIPVGQPVQLDLNSADVIHSFWVPSLAGKLDM
                     IPGRTNRLRIQADRPGRYRGQCAEYCGGPHARMAVFVVAEPPEQFDRWMQIQRRPATA
                     PADDIRRKGRDLFLAQCGACHAVRGTAAVGTRAPDLTHVAGREFIGAGLLPNNAGTLA
                     GWIASAQHLKPGSLMPAFDSLSGPELRALAAYLAGLS"
     gene            1890..4379
                     /gene="ctaD"
                     /locus_tag="VD791_07370"
     CDS             1890..4379
                     /gene="ctaD"
                     /locus_tag="VD791_07370"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HXV07831.1"
                     /translation="MSALPSPLPRPPGELEELKRLWSPPRGLRFITVVNNNYVGVFYV
                     GAALLFFVLAGILALLMRSQLALPDHRLLGPELYNQLFTMHGTTMMFLFAVPVVEAMG
                     VLLLPNMLGARDLPFPRLSAYAFWAYFVGGLLFFCSLFLGLAPKGGWFMYPPLATATY
                     SPGINEDFWLLGIGFIEISAIAGAIEIIVGILRTRAPGMTLARMPVFAWAMLVFALMV
                     VFAFPAVILGTLLLELERAFGWPFFSPELGGDPLLWQHLFWFFGHPEVYIIFLPAAGM
                     VSMIVPAMAQTPLVGQRLVVLALLAVGFLSFGLWVHHMFATGIPRLSLSFFQAASMAV
                     AIPSGIQVFAWIATLAKGRVRTSTPALFILGFLFTFVLGGLTGVMVAVVPFDWQVHDT
                     YFVVAHLHYVLIGGMVFPLFAAFYYWVPYTSRNALSERVGRWVFGLMFAGFHVAFFPM
                     HLTGLVGMPRRVYTYPAEMGWGTLNLISTIGAFILAAGVVLFVLDLGRRFRMSTEGNA
                     GNVWNAGTLEWLPSGNYSNRSIPIVSSPYPLWDQPGLAKNVEAGAYFLPGAVTGGRDT
                     LITSATDARPQWVLRLPMPGWAPLLGAWFTAACFLLLTFKLVWPALACALVAIAALVH
                     WGWGLDPPQDRQTVDIGGGLRLPMYVSGTSSHAWWAMVLLMLVGASLYGCLLFSYLYL
                     WVVSPEVWPETGPSLAYPLGAASLLLLSSLAVAHANRRVARHGDCSALAFAVPLLALA
                     AVLSILAQRDLLPVESAYAAMVHAVLTIDAFFVAAAIALALFALARRAAGRLDRVRRV
                     TFDNARLFWHYVVAQTLIGLLLVHGFPRVAN"
     gene            4381..4662
                     /locus_tag="VD791_07375"
     CDS             4381..4662
                     /locus_tag="VD791_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07832.1"
                     /translation="MVLLRMASGVLVWALHFGVIYGATGLACARGLGETVPWTVGTAT
                     LLAAAAAGVIILTHLSQEFARWMTAALAAVALVAIVWEGLAVLTVPACD"
     gene            4653..5009
                     /locus_tag="VD791_07380"
     CDS             4653..5009
                     /locus_tag="VD791_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HXV07833.1"
                     /translation="MRLAAVALCLALNACGDRQRVPPALSGDVENGRLLLRQFGCGSC
                     HAIPGVAVANGRVGPPLDGVANRVYLAGVLPNTPENMATFIRAPKAVDPLTAMPDLGV
                     TETHARHMVAYLYTLR"
     gene            5006..5809
                     /locus_tag="VD791_07385"
     CDS             5006..5809
                     /locus_tag="VD791_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV07834.1"
                     /translation="MKWLLGIVILATVCGGVVLYGGLYDVSATNQHLGPTYWLLDTGM
                     RRSVKRRTAGLAVPDLMDQQKLERGRVLYRSHCVSCHGAPGVAPEPHALGMTPTPAAL
                     VHTARAWSPAEIFLVVKEGIKMTGMPAWKYRMSDDDIWAVVAFLPEMARLTPRQYAAL
                     RAGPLTAAIETPAGAPDAARGKRAIYQYACVTCHAIPDIVGPNAPVGPPLAGIGARSM
                     IAGVLPNSPENLARWIRSPQEVAPLTAMPTLGVTQRDARDIAAYLATLR"
     gene            6118..>6393
                     /locus_tag="VD791_07390"
     CDS             6118..>6393
                     /locus_tag="VD791_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07835.1"
                     /translation="MELRYMAIVAATLALPAYASAANTPNEDAGRDMVPYSDREQMKS
                     WTDEKDRLEKALRVGEGKDYYRQELGNLGYRITAVNQDKRDYLEYEVV"
BASE COUNT          850 a         2198 c         2139 g         1206 t
ORIGIN      
        1 cagaccaagc cgagccctcg ccctccagtt cggcgcacca cggggtcatg agcagatcga
       61 tcccctgtaa ccagtcctct gtctcgtaat cggcctcatg tccgccggtt gcggcgtcgg
      121 cgccagcatg aacgggaagc tcaccgtctc gatgccacgt gtacttccta cgcgcgcgcg
      181 caactcttcc cggcgcggca tcgacgcctg gttcggtctg ctgatctgcg ccgacgcgtc
      241 ggcgcacgcc ggctccgcgc agagctggtg gagtttcgat ccctgggttt ggggcggctt
      301 cgcgctgctt ctcctgctgc gtccgcgccg acccgccagc ttcctgctcg cgggggcggc
      361 gctgttcctc gcgctgatct ggccgctcga cgtgctggcc gagaccagct tcgcggcaca
      421 tatggcccag cacatgttgc tgatcggggt tgccgcgccg ctgctcgtgc tgtcccgccc
      481 atcgctcggg cgggtgaagg ccatttccct aggcgggaag cgccgggttg cacggttact
      541 gcgcgggtgg agcacgctct cccggccgcc tactgccttt gccctgcacg cagccgccgt
      601 ctggatcggg cattggccgc gcgtgatcga ggccatgatc ctgcagcgtt gggtgcacat
      661 tctcgggcac gctgttctgc tggccaccgc gctggtcttc tggtggtcgc ttcgcagccg
      721 tggccgggcc ggagccggga atgcggcact ctggacgctg gccacgatgg tccacaccgg
      781 catgctcggt gcgctgctcg ccttcgcgcc gcgcgctttg tatccggcgt attccctctc
      841 cgaccagcag ctcgcaggat tgatcatgtg ggtgcccgga gcgctgtgct acctcgccgc
      901 cggcctggtc ttcgcggcgg cgtggttggg cgacgagcgg agaccggcgt agtgcggttg
      961 ctcgagggcg tccagtcggc gttgcacccg caggggcccg cggccgccgt cgtcgcggag
     1021 atgttctggg tgatgtgcgc cggcgcagcg atcattcttg tggcagtcac ggcgctcgcg
     1081 gcctacgcgg tgttcgcgcc gaggaaggac gcggcgatac tgtccggaag ctcgctgatc
     1141 gtggccggcg gcatagtctt tccggtgctc acactgtccg cgttgctcct gtacagcttc
     1201 gtacgcgcgg gatcgatcgc cccggcagcg ccgccgcccg ccgtgcgcat agaagtcgtc
     1261 ggcgagcaat ggtggtggcg cgtgcattac ctggacgtcg gcgggcgcag cgacttcgtc
     1321 acggccaacg agatccgcat accggtcggg cagccggtgc agctcgatct gaactcggcc
     1381 gatgtgatcc acagcttctg ggtgccgtcg ctagccggca agctcgacat gatccccggc
     1441 agaaccaatc gcctgcgcat ccaggcggat cgtcccggtc gctaccgcgg ccagtgtgcc
     1501 gagtactgcg gcggcccgca cgcccggatg gctgtgttcg tggtcgccga accgccggaa
     1561 cagttcgacc gctggatgca aatccagcgc cgacccgcaa ccgcgccggc ggacgacatc
     1621 cgccgcaaag gccgcgatct gttcctcgcg cagtgcgggg cttgtcatgc cgtgagaggc
     1681 accgcggcgg tgggcacacg ggcccccgat ctgacgcacg tggccggtcg tgaattcatc
     1741 ggcgccggcc tgctgcccaa caacgccggg acgctggccg gctggatcgc ctcggctcag
     1801 catctgaagc ccggcagcct gatgccggcg ttcgattcgc tgtccggccc ggagctgcgc
     1861 gcgctggcgg cctatctcgc gggtctgtcg tgagcgctct gcccagtccg ctgccccggc
     1921 cgccgggtga gctcgaagag ctgaagcggc tgtggagtcc gccgcgtgga ttgcgcttca
     1981 tcacggtggt caacaacaac tacgtgggcg tgttctacgt cggcgccgcg ctcctgttct
     2041 tcgtgctggc cggcatcctg gcgctgctga tgcgctccca gctcgcgcta ccggaccacc
     2101 gcctgctcgg tcccgaactc tacaaccagc tcttcaccat gcacggcacg acgatgatgt
     2161 tcctgttcgc ggtgcccgtg gtcgaggcga tgggagtgtt gctgctcccg aacatgctgg
     2221 gcgcgcgcga tctgccgttc ccgcggcttt ccgcctacgc gttctgggcc tatttcgtcg
     2281 gcgggctgct gttcttctgc agcctgttcc tggggctcgc tccgaagggc gggtggttca
     2341 tgtatccgcc gctcgccact gcgacctact cgcccggcat caacgaggac ttctggctgc
     2401 tgggcatcgg cttcatcgag atctcggcca tcgccggcgc aatcgagatc atcgtgggca
     2461 tcctgcgcac gcgtgctccc gggatgacgc tggcgcgcat gccggtattc gcctgggcga
     2521 tgctggtgtt cgcgctgatg gtggtcttcg cctttccggc cgtgattctc ggcaccctgc
     2581 tgctcgagct cgagcgcgcc ttcggctggc cgttcttcag ccccgaactg ggcggcgatc
     2641 cgctgctgtg gcagcacttg ttctggttct tcgggcatcc ggaggtctat atcatcttcc
     2701 tgcccgcggc cggcatggtc tcgatgatcg taccggcgat ggcgcagacg ccgctcgtgg
     2761 gtcagcgcct ggtcgtgctc gcgctgcttg ccgtaggctt cctgagcttc ggtctgtggg
     2821 tgcaccacat gttcgccacc ggcatcccgc ggctgtcgct gtcattcttc caagcggcca
     2881 gcatggcggt cgcgattccg agcgggatcc aggtgttcgc ctggatcgcc acgttggcga
     2941 aaggcagggt gcgaacgagc acgcctgcgc tcttcatcct cggcttcctg ttcaccttcg
     3001 tgctgggcgg gctcaccggc gtgatggtgg ccgtggtgcc cttcgactgg caggtgcacg
     3061 acacctactt cgtggtcgcg catctgcatt acgtgctgat cggcggcatg gtgtttccgc
     3121 tgttcgcggc gttctactac tgggtgccgt acaccagccg caacgcgctg tccgagcggg
     3181 tcggacgctg ggtgttcggc ctcatgtttg ccggtttcca tgtcgccttt tttccgatgc
     3241 acctgaccgg cctcgtcggc atgccgcggc gtgtctatac ctaccccgct gagatgggtt
     3301 ggggcacgct caacctgatc tccacgatcg gtgccttcat ccttgcggcc ggtgttgtgc
     3361 tgttcgtcct cgacctggga cggcgcttcc gcatgtcgac cgaaggcaat gcgggcaacg
     3421 tctggaacgc cggcaccctg gaatggctgc ccagcggcaa ctactcgaac cgcagcattc
     3481 ccatcgtcag cagtccctat cccctgtggg atcagcccgg tctcgccaag aacgtggagg
     3541 cgggggccta tttcctgccg ggcgccgtga ccggggggcg cgacaccctg atcaccagcg
     3601 cgacggacgc gagaccgcaa tgggtgctgc gcctgcccat gccggggtgg gcgccgctgc
     3661 tgggcgcctg gttcaccgcc gcctgctttc tcctcctgac gttcaagctg gtatggcccg
     3721 cgctcgcctg cgcgttggta gcgatcgcgg cgctcgtcca ctggggctgg ggcctcgatc
     3781 cgccgcagga tcgccagacg gtcgacatcg gcggcggact tcggctcccg atgtatgtca
     3841 gcggcacctc gtcgcacgct tggtgggcca tggtgctgct catgctggtg ggggcgtcgc
     3901 tgtacggatg cctgctcttc tcgtatctgt atctctgggt cgtttcaccg gaggtgtggc
     3961 ccgaaaccgg cccgtcgctc gcctatccgc tcggcgccgc ttcgctcctc ctgctgagca
     4021 gcttggcagt ggcgcatgcc aaccgcaggg ttgcgaggca cggggactgc agcgcgctcg
     4081 cgtttgccgt gccgctgctc gcgctggcgg cggtgctgag catccttgcg cagcgcgacc
     4141 tgcttcccgt cgagagcgct tacgccgcga tggtccatgc ggtgctgacg atcgacgcat
     4201 tcttcgtcgc ggcggcgatc gcgctggcgc tgttcgcgct cgcccgccgc gccgccggcc
     4261 gcctggatcg ggtccggcgc gtgactttcg acaacgcgcg gctgttctgg cactacgtcg
     4321 tcgcccagac cctgatcggg ttgctgctgg ttcacggctt tccgcgcgtg gcgaattgac
     4381 atggtgctcc tgcgcatggc ttccggcgtc ctcgtgtggg cgctgcactt cggcgtgatc
     4441 tacggcgcga ccgggctggc ctgcgcccgc ggcctcggcg agacggtgcc ctggaccgtc
     4501 ggcacggcga cgctgttggc tgccgcggca gccggcgtga tcattctgac gcacttgtcg
     4561 caggagttcg cgcgctggat gaccgcagcg ctcgccgccg tggcgctggt ggcgatcgtt
     4621 tgggagggac tcgccgtgct cacggtgccc gcatgcgatt agcggccgtc gcgttgtgcc
     4681 ttgcgctgaa tgcctgcggc gatcggcagc gcgtgccgcc ggcgttgagc ggcgacgtcg
     4741 agaacggacg cctactcctg cgtcagttcg gctgtggaag ctgccacgcc attcccggcg
     4801 tggcggtcgc caacggccgc gtcgggcccc cgctcgacgg cgtggccaac cgcgtttacc
     4861 tcgcgggcgt actccccaac accccggaga acatggcgac cttcatccgc gcgccgaagg
     4921 cggtcgaccc gctcaccgcc atgcccgatc tgggggtgac cgaaacgcat gcgcgccaca
     4981 tggtggccta cctctacacc ctgcgatgaa gtggctgctc ggcatcgtga ttctcgcaac
     5041 cgtgtgcggc ggagtcgtgc tgtatggggg cctgtacgac gtctcggcca ccaatcagca
     5101 cctcgggccg acctactggc ttctcgacac cgggatgcgg cgctccgtga agcggcgtac
     5161 cgccggcctc gcggttccgg atctgatgga tcagcagaag ctcgagaggg ggcgtgtcct
     5221 ctatcgcagc cactgtgtgt cgtgccatgg cgcacccggc gtcgccccgg agccgcatgc
     5281 gctgggcatg acgcccactc cggccgcgct ggttcatacg gcgcgcgcct ggtcgccagc
     5341 cgagatcttc ctggtggtca aagagggcat caagatgacc ggcatgcctg cgtggaagta
     5401 ccgcatgagc gacgacgata tctgggcggt ggtggcgttc ctgccagaaa tggcgcgcct
     5461 cacgcctcga caatacgcgg cgctgcgcgc cggcccgctg accgccgcaa tcgaaactcc
     5521 cgctggcgcc ccggacgccg cccgcggcaa acgcgccatc taccaatacg cgtgcgtaac
     5581 ctgccacgcc atccccgaca tcgtcggccc gaatgcgccc gtgggcccgc cactcgcggg
     5641 aatcggggcg cgctcgatga tcgccggcgt gctgccgaac tcgcccgaga acctggcacg
     5701 ctggatccgc tcgccgcagg aagtcgctcc gctcaccgcc atgccgaccc tcggtgtcac
     5761 gcagcgcgat gcgcgcgaca tcgcagcata tctggccacg ctacgctagc tcgcgcgccg
     5821 catgcagccg ccgcggcgcc gggcctcatc gacaccggcc gtcgcgcagt gggctgcaga
     5881 accgccaggt aaaaagttcc ggcctgtggc gcttccttcc aatgctgctg cgaggaaccg
     5941 aaccctctcc cgtcgctgca cgaggccgcg aaacgcacta cggggccgac tgcgcgcgag
     6001 cgccgagatc cgatgcagcg cagccgccca tcgcgacgcg ggaccacggg cacgcgcaat
     6061 gcaaacagga acgtacgggt cgggcgcgcg gcgtccccct ttttccagga gacgagcatg
     6121 gaattgcgtt acatggcgat cgtggcggca accctcgcgc tgcccgcgta cgcatctgct
     6181 gccaacacac cgaacgagga tgccggccgg gacatggtcc cgtacagcga tcgcgagcaa
     6241 atgaagagct ggaccgacga gaaggaccga ctcgagaagg ctctgcgagt gggcgaaggc
     6301 aaggactatt accggcagga gctgggcaac ctgggctatc gcatcaccgc ggtcaatcag
     6361 gacaagcgcg attacctgga gtacgaggtg gtg
//
LOCUS       DASPGE010000232        21434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1466518, whole genome shotgun sequence.
ACCESSION   DASPGE010000232 DASPGE010000000
VERSION     DASPGE010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21434
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466518"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VD791_07395"
     CDS             complement(<1..432)
                     /locus_tag="VD791_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HXV07836.1"
                     /translation="MTGSQLYLRLLGHVKPYWRMFALSILGMLISAATEVALPAAAKP
                     FLDGTFVDRDPVLMRWVPFVIVLVFIARGIGTFMGSYGSAWVGQRVVMDLRSRMFDKL
                     LQLPQRYYGDHLSGNLISKFTFDVVQVATAVTHVVTALVRDS"
     gene            complement(442..1872)
                     /locus_tag="VD791_07400"
     CDS             complement(442..1872)
                     /locus_tag="VD791_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HXV07837.1"
                     /translation="MLHTELLINGKFIRGQGAEESIVNPATGVQLAKVAEASPQQVDE
                     AVRAAQAAHAGWGQSTPQERSLLLLKLADRIEQEAAAFAELESLNCGKPRVRVLNDEM
                     PAIVDCFRYFAAAARCLPGSNTDEYLPNHTSMIRRDPIGVVAQIAPWNYPLMMAAWKI
                     APALAAGNAVVIKPSEQTPLTTLKLARLCAELFPAGVLNVVTGRGATVGSPLINHPGV
                     RMVSVTGSVATGQKVIEAAVASLKRTHLELGGKAPVIVFDDADLSAVVASIRMAGFYN
                     AGQDCTAACRIYAADKIYDNLVADLGSAVRSIKFGAPENDDSEIGPLITAKHRERVTG
                     FVERAVGSKHIELVAGGSAAAGGGFFYQPTLIAGALQTDEIVRKEVFGPVVSVTRFRD
                     ADQAIAWANDSDYGLASSVWTGDVRKAMKVASRLEYGCTWINTHFTLVNEMPHGGVKM
                     SGYGKDLSMYGLEDYTVVRHVMVNFG"
     gene            complement(1883..2698)
                     /locus_tag="VD791_07405"
     CDS             complement(1883..2698)
                     /locus_tag="VD791_07405"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV07838.1"
                     /translation="MRSRTGGDPGWGLRASAATAVLFLHVPLAFIVLYAFSSDAKTFS
                     FPPPGLTTQWFGIAFERPDIRAALGLSLRVAAVSTLTAMALGTLAAAAVWRSRFFGRE
                     AISLLLILPIALPGIITGISLRSAIGLLEIPFSFWTIVIGHATFCVVVVYNNVLARFR
                     RTSFAQIEASMDLGADGFQTFRYVILPNIATALLAGGMLAFALSFDEVIVTTFTAGQQ
                     STLPIWMLTELVRPRQRPVTNVVAVFVIAVTFLPILAAFYLTRDTHEVAGGGK"
     gene            complement(2688..3623)
                     /locus_tag="VD791_07410"
     CDS             complement(2688..3623)
                     /locus_tag="VD791_07410"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV07839.1"
                     /translation="MTAAALQAPAPLQRLSNALYLQPRLLLFLLLTPPLLWLGVIYLG
                     SLFALLAQSFFYIDEYSGMVVREFSLATWRQLFTLANLDIVVRTTGMAAAVTVGAVLL
                     GFPLAYYMVRYASTRMKALLYLGVMLPLWSSYLVRVYSWKLILAKEGIITWLAQQLHL
                     TWLLEGMLALPVIGGPSLSTSDIGTFLVFLYVWLPFMILPVVAALERVPRTLIEASAD
                     LGAHPGATFRRVIWPLALPGVVAGSIFTFSLTLGDYIIPGIIGSSHPFIGQAVYAHQG
                     TAGNVPLAAALTIVPIAIMAAYLTIAKRLGAFDAL"
     gene            complement(3620..4657)
                     /locus_tag="VD791_07415"
     CDS             complement(3620..4657)
                     /locus_tag="VD791_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV07840.1"
                     /translation="MSTAIPAVRFNGVRRQFGEVRAIDDVGFEIADGEFFSLLGPSGS
                     GKTTCLRLIAGFEQPDAGTIEIHGVNVAGVPPYQRDVNTVFQDYALFPHMSVSQNVEY
                     GLMIRKVPAAERSRRAEEMLELVKLAGMRGRKPAQLSGGQRQRVALARALINRPRVLL
                     LDEPLGALDLKLREAMQVELKALQRQVGITFVYVTHDQGEALSMSDRVAVFNLGRIEQ
                     IDTPAALYEHPATAFVAGFVGVSNLCEGEVAKRLSGSAEPFAIRPEKIRIAREDAPTP
                     DGWVGAGGRVESIVYLGAATRYTVALDGGGSLIVLHQNADDRDHGPVTGARVRLAWQA
                     RHQQRLGGQRA"
     gene            complement(4732..5895)
                     /locus_tag="VD791_07420"
     CDS             complement(4732..5895)
                     /locus_tag="VD791_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV07841.1"
                     /translation="MTSAFRTSLTALAAALVGAALAASAQAQMTKIGKGEGTVDIVAW
                     PGYIERGDTDKNFDWVTDYEKKTGCKVRVKTANTSDEMVALMNEGGFDLVTASGDASL
                     RLIAGGRVQEVNTKLVPSFKNVDKRLQQAPWHYVNGKHYGVPYQWGYNVLAYNTKVFS
                     QPPKSWDVVFKEMTLPDGKSNKGRVQAFDGPIYIADAALYLMKTKPELGIKNPYELNE
                     DQYKAALGLLREQRKLVSRYWHDAFIQIDDFKNEGVVASSSWGFQVNLLQGEKQPIGS
                     TVPVEGATGWADTTMMHANAPHPNCAYMWLEHSVNPKLQGDLSAWFGSVPAVPAACKG
                     NKLLTDEGCARNGMNEFERIWFWRTPVTNCGGGRTCVPYYRWVTDYIAILGGR"
     gene            complement(6051..8132)
                     /locus_tag="VD791_07425"
     CDS             complement(6051..8132)
                     /locus_tag="VD791_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXV07842.1"
                     /translation="MDVSRLGPALLAVCCGHLLAQSDPVEVTTPATVITAARFPESAA
                     EGAVNVTVIDAEQIARSQATNLPELLVLEAGIHTRDLYGNSAAGAAVDLRGFGAAAGQ
                     NTLILVDGRRLNDIDLAGVQWSSVPLSAIERIEIVRGSGAVQYGDGATTGVINIITRS
                     PAAAQYGANLRTRLGSFETREAQVGGNFAGQSVGLALGVYDYESDGYRDNNRNETDSA
                     YLDARWMPERWTVSFKAGADSEDLRLPGARLVQPSAGIDELESDRRGTSTPLDFATRD
                     GRRAAVELEHQAPGFDLNLGLGYRDKAQTSYFDFGGFPDYREVDLDVWSFTPRLRVPF
                     GAGGARAALVAGVDVYTWDYALRTSNAQTNIGQPINRVSADQENRAVYLRLTAQLTAA
                     TSASGGWRYERQSIDAQDVFDPTAPGAAFGSGAMAGTQTESEQAWELALRHALSPAFA
                     LSARAGRGFRFATVDEIYETSALFTREFQFLSPQTSMGYDLGVEYADPTLFARATVFQ
                     LDVDDEIHLDPYSTGVGNTNLPPTRRRGLELEARWRPTQSLTAQAAYTYTDAWFREGT
                     LPGSAITQQNVDIAGKTVPLVPEHQVTAQAVWQMTPAWRLTGAARHVSSQFMENDEGN
                     TGVKIPAYSVLDLRLDFLEGPLTLTAAVNNVLDEEYYNYAVRSQFVPDRYNAYPLPER
                     TFWVALQLRLP"
     regulatory      complement(8235..8539)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8684..8884
                     /locus_tag="VD791_07430"
     CDS             8684..8884
                     /locus_tag="VD791_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF904 domain-containing protein"
                     /protein_id="HXV07843.1"
                     /translation="MEAELNSLDEKISQLIQLCHRLRTDNSELRQQLAAAQNQSKQLT
                     EKIEAARRRLESLLSRIPEDAA"
     gene            8881..9192
                     /locus_tag="VD791_07435"
     CDS             8881..9192
                     /locus_tag="VD791_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HXV07844.1"
                     /translation="MTREARAIEVSILDRSFRIACSEEEEPGLRQAVEYVDRKMREIR
                     DASKTAGHERIAVMAALNIAHELLSLRVGGGFDMGQFKRRMHAMAAAIDEAMSTQDEL
                     F"
     gene            9451..9906
                     /locus_tag="VD791_07440"
     CDS             9451..9906
                     /locus_tag="VD791_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HXV07845.1"
                     /translation="MRYWLMKSEPDVFGVDDLAAAPKKTTAWWGVRNYQARNFMRDDM
                     RLGDLAFFYHSSCPQPGIAGIMRVSRLAYPDETQFDPRSEYHDPGSDRDDPRWVNVDV
                     TLVRKIRLLPIQELRAHRALAGMRVLQRGNRLSITPVTAREWEFIERLL"
     gene            complement(9903..11399)
                     /locus_tag="VD791_07445"
     CDS             complement(9903..11399)
                     /locus_tag="VD791_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HXV07846.1"
                     /translation="MNSNLYAHIERAAPADLGDTVLFDSGAEMSWRALQEQTARFAAL
                     LRSLGLQRGDRVAAQVEKSPQSLFLYLACLRAGVVYLPLNTAYQRAELSYFLSDAQPR
                     VVVCRPQSEAEVRGLAGAAEVLTLDEAGAGSLAGAARDQPVVFETAQVAADDLAAIIY
                     TSGTTGRSKGAMLTHRNLLSNGRVLVQAWGFTAGDVLLHALPMFHVHGLFVANHCALL
                     AGARMLWHRKFDARALLRDLPRATVLMGVPTFYTRLLAEPGFTAQACGGVRLFVSGSA
                     PLLPDTFEEFERRTGQRILERYGMSEAGMICSNPLHGERRAGSVGPPLPGVSVRVADE
                     RDRPLPAGRVGGIQIRGENVFRGYWRMPEKTREEFSADGWFRTGDVGVFDADGYLSIV
                     GREKDLIITGGYNVYPKEIELAIDALPGVLESAVVGLPHPDFGEAVTAVVVPRAGAVL
                     SPEDIIQTLKRELANYKVPKRVILVPDLPRNAMGKVQKSVLRERYGAG"
     gene            complement(11396..12718)
                     /locus_tag="VD791_07450"
     CDS             complement(11396..12718)
                     /locus_tag="VD791_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXV07847.1"
                     /translation="MRFSELSPEQRVAALLDGNVMQPRSAGAMLTASGVLRGRRVYVA
                     ATDPAIARGAIGVAEADALAAVLRDAREHTAPALLVLDSAGARVDEGLRALGAFRRLF
                     REALQAKLAGTPLLALLGPICFGGASLLACLCDRRIYLAQTRIATSGPALIESASGKD
                     RLDAADRSAVQALIGAQARVPLHPGDAIADDAPAAMRDAAARAVEGERPALVLAGEHA
                     RLRARLADAGMLRHASAQATDSAALARVLPTGYVPTIAGSVFLAQPAERSGKAVFLGS
                     VGAPVDAADCWHLADWLLALGDSHPGSPLVLVLDAEAHAATVENERVLLADYLVHLSL
                     CGAARARSAPRTVLWLPGAASGASYVAFAAPVDRVCALPAARIAILPPAAVRQILGDE
                     ASTAASPRDWIQTGVADALLDGLQHFAEAGDALRAGSQGSSTRRLSDS"
     gene            complement(12729..13856)
                     /locus_tag="VD791_07455"
     CDS             complement(12729..13856)
                     /locus_tag="VD791_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HXV07848.1"
                     /translation="MTATLDAVRLTQDILRINTVNPPGNEEACARVLGRLLENAGYRI
                     RYHSFGPGRVNLLADIGGAERPPLCLTGHVDVVPLGAAAWRKDAFAGETDAGRLYGRG
                     SSDMKSGVAACVAAAVTMAPHLERTPGVTLVITAGEETGCEGAFHLLHDGALGRAGAV
                     VVAEPTSNYPFVGHKGAFWLRARTRGVTAHGSMPEKGDNAVYKAARAIGKLETFRFSN
                     APHPLMGQATLNVGTIQGGLNINSVPDQATIGIDIRTVPTVDHRALLADLARQLGPEV
                     ELEPMMDVQAVHTDPEQPWVQSVFEVMAPLLGARPVARSATYFTDAAALSQAYRAPPT
                     VILGPGEPQMAHQTDEYCVIDRIHQAVGAYQEIIRRWCLAA"
     gene            complement(13893..14600)
                     /locus_tag="VD791_07460"
     CDS             complement(13893..14600)
                     /locus_tag="VD791_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV07849.1"
                     /translation="MSELVVEDLYTAYGRADVLCGVSLRARPGEITCILGSNGAGKST
                     LIRSILGLTPARRGRILFGGEDLAGRPTHEVVARGIACIPEGRKVFPKMTVLENLLAG
                     AYRERDARARAARIEQVYAGFPRLRERAAQLAGTLSGGEQAMLSIGRGLMADPRLLIV
                     DEPSLGLSPLLVKENFAVIQRVNQAGVTILLVEQNVKQTLSIAHRGYVLSQGRVVAEG
                     SAEALRSDPEVRRAYFG"
     gene            complement(14704..15489)
                     /locus_tag="VD791_07465"
     CDS             complement(14704..15489)
                     /locus_tag="VD791_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV07850.1"
                     /translation="MNEAASLLRCEGLSVRFGGLTALNGLDLRLHEGEILGLIGPNGS
                     GKTTFFNALTGLYRAASGRAWFAGAEITGLRPQAIYDAGIARTFQRSRLCLPLSVFDN
                     LMIGNHRRLSLGLWFNLFRRSRFAQELQRHVERARTLLAAFHPQLPERMFEPAGALGM
                     IDRRRVEVCRALISEPRLLLLDEPSAGMTHDETRELMDDILAARERLPGLSIILIEHE
                     MGVIERVSERCVVLNFGEKICEGPFAAVVADPQVQEAYLGGSS"
     gene            complement(15486..16652)
                     /locus_tag="VD791_07470"
     CDS             complement(15486..16652)
                     /locus_tag="VD791_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV07851.1"
                     /translation="MTTHAVQPAALWPALATTAAIAGFAYLFLRVEAQWEVVALTAGA
                     LVALLALGRTRLGAAVGAAWSVRPAPLAWGALLATSVVVALFAEDHFALLMIATVLLY
                     GVACLGLTIQFGYAGVVNFSGAAFFGIGGYTAAVLATHTALPHLLVVAAGGVLAGAIG
                     SLLVLPLLRTRGHYAALITIAFAILFKTFLEVNDALGGPQGLKVPGLRMLGWSFNDNI
                     ALGEYTELSFYLDYAVFSLLLLAGALLLVRRLERSWLGLSLDAVRIDETAASVFGFEV
                     ARWKILAFTLGNVLAGTAGAVYAMMTGFVAPASFTLAESLILVSIVILGGLGNPVGVL
                     AAAALVVMLPEKLQVIQEYRFLLFSAFVILVLLFRPEGLLPRKLRQYLPGWGRA"
     gene            complement(16649..17560)
                     /locus_tag="VD791_07475"
     CDS             complement(16649..17560)
                     /locus_tag="VD791_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV07852.1"
                     /translation="MSLLGQYVFNGLMLGTIYAMVAVGFTLFFGVLDVIKFSHGDVLM
                     VGAFTGLVAYLGLSALGVESNALLSLGVMLTAVPLIALLGAAMARWLVLPLRGSPPIN
                     VLLITLMAGTVLREAIRLFYPAGANPKPFPRLLPTDAVSFGALTLRFDSAILIALGLA
                     SIVAVHLLVTRTRLGLAIRAVAQDSETAQVMGMNFQRVVLVTFAIGSALAALAGVTAG
                     LYYNEINFGMGLLLGVIGFSAAVVGGLGNLYGAILGGFLFAGLQTLGAVWFAVPAYKD
                     VFAFAVVILVMTWRPTGLIAEQSAERV"
     gene            complement(17604..18761)
                     /locus_tag="VD791_07480"
     CDS             complement(17604..18761)
                     /locus_tag="VD791_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HXV07853.1"
                     /translation="MLPRPLRAALCCAALLAAGAAGAKEAVKLVFIGPLTGGVSANGL
                     GGRNATDLAVRLRNAEAKARYRYELVSLDDECKPNIGVQVATKAAADRSIVAGVTHYC
                     STVAMATVDVYHKFGLPVVVWGAVLPDITYANDYPEVHRVNGTMINQNETAARFMTEL
                     KYRRWAVIHDTTDYGKGHNKYFSEYLAKNGGRILGTFGVTADQQDFSAELTKIKDLKP
                     EVVYFGGITPLGVRIRTQMDKLGVKAVFQGTSGIVSDAYIEGLGPLAEGSLAFREGAP
                     IEKLPGGKFFLERYNAQKYNDPPEAYGAFAFAAANLVMDAIEKVGPDRKKVRSELNRT
                     RDYQTVVGTITFDDHRQNAVPLITKFVVQDGRWIVWEDSDYAKGKRKLVQP"
     gene            complement(18992..19411)
                     /locus_tag="VD791_07485"
     CDS             complement(18992..19411)
                     /locus_tag="VD791_07485"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HXV07854.1"
                     /translation="MANTIHIDVVSAEEGIYSGEAEFVVLPGEAGELGIYPRHTPLIT
                     RIKPGSVRIKAAGKDEEELIFVAGGILEVQPNLITVLADTAIRGHDLDEAKAIEAQQR
                     AEEARRNAKGKEELAVVEGELAMLAAQLAAIRRLRKK"
     gene            complement(19515..20921)
                     /gene="atpD"
                     /locus_tag="VD791_07490"
     CDS             complement(19515..20921)
                     /gene="atpD"
                     /locus_tag="VD791_07490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015420207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HXV07855.1"
                     /translation="MANAQGTIVQTIGAVIDIEFPREGMPKVYDALLLEDGDAASFAE
                     KGLTFEVEQQLGDGVVRCIALGSSDGLRRGMKVRGTGAPISVPVGPGTLGRIMDVLGR
                     PIDEAGPIQADQRRAIHQPAPKFDELSPSVELLETGIKVIDLVCPFAKGGKIGLFGGA
                     GVGKTVNMMELINNIAKQHAGLSVFAGVGERTREGNDFYHEMKESNVLDKVAMVFGQM
                     NEPPGNRLRVGLSGLTMAERFRDDGRDILLFIDNIYRYTLAGTEVSALLGRMPSAVGY
                     QPTLAEEMGRLQERIVSTKTGSITSVQAIYVPADDLTDPSPATTFAHLDATVVLSRDI
                     ASLGIYPAVDPLDSTSRQVDPNVIGEEHYNTTRQVQATLQRYKELRDIIAILGMDELS
                     PEDKLTVARARKIQRFLSQPFHVAEVFTGTAGKFVPLKETIKGFKMIVNGECDHLPEQ
                     AFYMVGPIEEAIEKAKTL"
     gene            complement(20956..>21434)
                     /locus_tag="VD791_07495"
     CDS             complement(20956..>21434)
                     /locus_tag="VD791_07495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HXV07856.1"
                     /translation="VGANIVSQVTALGDRPHMERLIGALKVMLDGYASDRFDRLLMFY
                     TRFINTMKQEPVMEQLLPLAGDRLGAPEGSWDYIYEPEAKAVLDQVLTRYIEALIYQA
                     VAENMASEQSARMVAMKAASDNAETLIDELTLIYNKNRQAGITKELSEIVGGAAAV"
BASE COUNT         3463 a         7351 c         7311 g         3309 t
ORIGIN      
        1 cgagtcgcgc accagtgcgg tgaccacgtg ggttacggcc gtcgccacct gcaccacgtc
       61 gaaagtgaac ttggagatca ggttgccgct caagtggtcg ccgtagtagc gctgcggaag
      121 ctgcagcagc ttgtcgaaca tgcggctgcg caggtccatc accacccgct gacccaccca
      181 ggcgctgcca tagctgccca tgaaggtgcc gatgccgcgc gcgatgaaca ccagcacgat
      241 gacgaacgga acccagcgca tcagcaccgg atcgcgatcg acgaaggtgc catcgaggaa
      301 gggcttggcc gccgccggca gcgccacctc ggtcgcggcc gagatcagca tgcccaggat
      361 ggaaagcgcg aacatccgcc agtagggctt cacgtgcccg agcaggcgca ggtacagctg
      421 ggagccggtc atgccggcac attacccgaa gttcaccatc acatgccgca cgacggtgta
      481 gtcctccagg ccgtacatcg acaggtcctt gccgtagccg gacatcttca ccccgccatg
      541 cggcatctcg ttgaccaggg tgaaatgggt gttgatccag gtgcagccgt actccaggcg
      601 gctcgccacc ttcatcgcct tgcgcacgtc gccggtccac accgacgagg ccagcccgta
      661 gtcggagtcg ttcgcccacg cgatggcctg gtcggcgtcg cggaagcgcg tcaccgacac
      721 caccggcccg aaaacctctt tgcgcacgat ctcgtcggtc tgcagcgcgc cggcgatgag
      781 cgtgggctgg tagaagaagc cgccgcccgc cgccgccgag ccgccagcga ccagctcgat
      841 gtgcttgctg cccaccgcgc gctcgacgaa cccggtgacg cgttcgcgat gcttggccgt
      901 gatcagcggc ccgatctcgc tgtcatcgtt ctcgggcgca ccgaacttga tggagcgcac
      961 ggcgctaccc agatcggcga ccaggttgtc gtagatcttg tcggccgcat aaatgcggca
     1021 ggccgcggtg cagtcctggc cggcgttgta gaagccggcc atgcgaatgc tcgccacgac
     1081 cgccgacagg tcggcgtcgt cgaacacgat gaccggagcc ttgccgccta gctccaggtg
     1141 ggtgcgcttc aggctcgcca ccgccgcctc gatcaccttc tggccggtgg cgacgctgcc
     1201 ggtcaccgac accatgcgca cgcccgggtg gttgatgagc ggagagccga ccgtggcacc
     1261 gcggccggtc acgacgttca gcaccccggc ggggaacagt tcggcacaca ggcgcgcaag
     1321 cttcaacgtg gtgagcggcg tctgctcgga gggcttgatc accaccgcgt tgccggcggc
     1381 gagcgcgggc gcgatcttcc acgccgccat catcagcgga tagttccacg gcgcgatctg
     1441 cgccaccacg ccgatgggat cgcgccggat catgctggtg tggttgggca ggtactcgtc
     1501 ggtattgctg cccggcaggc agcgggcggc ggcggcgaag tagcggaagc agtcgacgat
     1561 ggccggcatc tcgtcgttca gcacgcgcac gcgcggtttg ccgcagttga gcgactccag
     1621 ctccgcgaag gccgcggcct cctgctcgat gcgatcggcc agcttgagca gcagcagcga
     1681 acgctcctgc ggcgtgcttt gcccccagcc cgcatgggcg gcctgcgcgg cgcgcaccgc
     1741 ttcgtccacc tgctgcgggc tggcctcggc gaccttggcg agctgcacgc ccgtggcggg
     1801 attgacgatc gactcctcgg cgccctggcc gcggatgaat ttgccgttga tcagaagctc
     1861 ggtgtgcagc atgtgcgctc tcctatttgc cgccgccggc gacctcgtgc gtgtcgcgcg
     1921 tgaggtagaa ggcggccaga atcgggagga aggtcacggc gatgacgaac accgcgacca
     1981 cgttggtgac cggccgctgg cgcgggcgca ccagctcggt gagcatccag atcgggaggg
     2041 tgctctgctg gccggcggtg aaggtggtca cgatcacctc gtcgaaggac aaggcgaagg
     2101 ccagcatgcc gccggcgagc agcgccgtgg cgatgttggg caggatcacg tagcggaacg
     2161 tctggaagcc gtcggcgccc aggtccatgc tcgcctcgat ctgcgcgaag gaggtgcggc
     2221 ggaaacgcgc cagcacgttg ttgtacacca ccacaacgca gaacgtggcg tggccgatga
     2281 cgatggtcca gaagctgaac gggatctcca gcaggccgat ggcgctgcgc aggctgatgc
     2341 cggtgatgat gcccggcagc gcgatcggca ggatcagcag cagcgagatc gcttcgcgtc
     2401 cgaagaaacg cgagcgccac accgcagccg cggccagggt gccgagcgcc atagcggtca
     2461 aagtggacac ggccgccacc cgcagcgaca gtcccagcgc ggcgcggatg tccgggcgct
     2521 cgaaggcgat gccgaaccac tgggtggtca gtcccggcgg cgggaaggag aaggtcttgg
     2581 cgtcgctgct gaaggcgtag agcacgatga acgccagcgg cacgtgcagg aacagcaccg
     2641 ccgtggcggc gctcgccctc aacccccacc ccggatcgcc gcccgttcta gagcgcatcg
     2701 aacgctccca ggcgcttggc gatggtcaga tacgccgcca tgatggcgat cggcacgatg
     2761 gtgagcgcgg cggcgagcgg gacgttgccg gcggtgcctt gatgcgcata cacggcctgc
     2821 ccgatgaacg ggtgcgagct gccgatgatg ccggggatga tgtagtcgcc cagggtgagc
     2881 gagaaggtga agatggagcc cgccaccacg cccggcagcg ccagcggcca gatcaccctg
     2941 cggaaggtgg cgcccggatg cgcgcccagg tctgccgagg cctcgatcag cgtgcgcggc
     3001 acgcgctcga gcgcggccac caccggcagg atcatgaacg gcagccacac gtacaggaac
     3061 accaggaagg tgccgatgtc ggaggtggac agcgacggcc cgccgatcac cggcagggcc
     3121 agcatgccct cgagcagcca ggtcaggtgc agctgttgcg ccagccaggt gatgatgcct
     3181 tccttggcga ggatcagctt ccaggagtac acccgcacca ggtagctgga ccacagcggc
     3241 agcatcacgc ccaggtacag cagcgccttc atgcgcgtgc tcgcgtagcg caccatgtaa
     3301 taggcgagcg ggaagccgag cagcaccgcg cctacggtga ccgccgcggc catccccgtg
     3361 gtgcgcacga cgatatccag gttggccagc gtgaacagct gccgccaggt ggcgagcgag
     3421 aactcgcgca ccaccatgcc cgaatactcg tcgatgtaga agaagctctg cgccagcagc
     3481 gcgaacaacg agcccaggta gatcaccccg agccacagca gcggcggggt gagcagcagg
     3541 aacagcagca ggcgcggctg caggtacagc gcgttggaca gacgctgcag cggcgcgggt
     3601 gcctgcagag cggcggccgt cacgcgcgtt gcccgccgag acgttgctgg tgccgcgcct
     3661 gccaggcgag gcgcacgcgt gcacccgtta ccggaccgtg gtcgcggtcg tcggcgttct
     3721 gatgcagcac gatgaggctg ccgccgccgt ccagcgccac cgtgtagcgg gtggcggccc
     3781 ccagatacac gatcgactcc acgcggccgc cggcgcccac ccagccgtcg ggcgtcggcg
     3841 cgtcctcgcg ggcgatgcgg atcttctccg ggcggatggc gaacggctcc gccgagcccg
     3901 acaggcgctt cgccacctcg ccctcgcaca ggttggacac gccgacgaag ccggcgacga
     3961 aagcggtggc cgggtgctcg tagagggccg cgggcgtgtc gatctgctcg atgcgcccga
     4021 ggttgaacac cgccacgcga tcgctcatcg acagcgcttc gccctggtcg tgcgtgacgt
     4081 agacgaaagt gatgcccacc tggcgctgca gcgccttcaa ttccacctgc atcgcctcgc
     4141 gcagcttgag gtcgagcgcg cccagcggct cgtccagcag caggacgcgc ggccgattga
     4201 tcagcgcgcg cgccagcgcc acgcgctgcc gttgcccgcc cgagagctgc gccggcttgc
     4261 ggccgcgcat cccggcgagc ttgaccagct ccagcatctc ctccgcgcgc cggctgcgct
     4321 cggccgccgg caccttgcgg atcatcagcc cgtactccac gttctggctc acgctcatgt
     4381 gcgggaacag ggcgtagtcc tggaacacgg tgttgacgtc gcgctggtac ggcggcacgc
     4441 cggcgacgtt cacgccgtgg atctcgatgg tgccggcgtc gggttgctcg aagccggcga
     4501 tcagccgcag gcaggtggtc ttgcccgaac cggacgggcc gagcagggaa aagaactcgc
     4561 cgtcggcgat ctcgaagccg acgtcgtcga tggcgcgcac ctcgccgaac tggcgccgca
     4621 cgccgttgaa gcggaccgcg ggaatggcgg tggacataaa acagcgggcc gggccccgcg
     4681 atcgatcccg gctgggcccg gccctgcgtt tggttcagcg caatcccggg gtcagcgccc
     4741 gccgaggatg gcgatgtagt cggtcaccca gcggtagtag ggcacgcagg tacgcccgcc
     4801 cccgcagttg gttaccggcg tgcgccagaa ccagatgcgc tcgaactcgt tcatcccgtt
     4861 gcgcgcgcaa ccctcgtcgg tcagcagctt gttgccttta cacgccgcgg gtaccgccgg
     4921 caccgagccg aaccaggcgg acaggtcgcc ctgcagcttg ggattgacgg agtgctccag
     4981 ccacatgtac gcgcagttcg gatggggggc attcgcgtgc atcatcgtcg tgtccgccca
     5041 cccggtggcg ccttccaccg gcaccgtgct gccgatgggc tgcttctcgc cctgcagcag
     5101 gttcacctga aagccccagg acgaagaggc gaccacgccc tcgttcttga agtcgtcgat
     5161 ctggatgaag gcgtcgtgcc agtagcgcga caccagcttg cgctgctcgc gcagcagccc
     5221 gagcgcggcc ttgtactggt cctcgttcag ctcgtagggg ttcttgatgc ccagctcggg
     5281 cttggtcttc atcaggtaca gagcggcgtc ggcgatgtag atcggcccgt cgaaggcctg
     5341 cacgcggccc ttgttcgact tgccgtccgg cagggtcatc tccttgaaca ccacgtccca
     5401 gctcttgggc ggctgcgaga agaccttggt gttgtaggcc agcacgttat agccccactg
     5461 gtagggcaca ccgtagtgct taccgttcac gtaatgccag ggcgcctgct gcaggcgctt
     5521 gtcgacgttc ttgaagctgg gcaccagctt ggtgttgacc tcctgcacgc ggccgccggc
     5581 gatcaggcgc aggctggcgt cgcccgacgc ggtgaccagg tcgaagccgc cctcgttcat
     5641 cagggccacc atctcgtcgg aggtgttggc ggtcttcacc cgcaccttgc acccggtctt
     5701 cttctcgtag tcggtgaccc agtcgaagtt cttgtcggtg tcgccgcgct cgatatagcc
     5761 cggccaggcg acgatatcca ccgtcccctc ccccttgccg atcttggtca tctgcgcctg
     5821 ggcggaggcg gcgagggcgg cgcccaccag cgcggcggcg agcgcggtga gtgaggtccg
     5881 gaacgcggaa gtcatcttca gtctccttgt tgacttgttg ggtgccgtgc gaagctcccg
     5941 accgggtgcg gtcatgcgta caacatgccg ctccgggagg cggaggcatt gtagcaccgg
     6001 cctttgcggc gcaaccgggg ggcgcctcga tgcggccggg cgccggctga ctaggggagt
     6061 cggagctgca gcgcaaccca gaaagtccgc tccggcagcg ggtaggcgtt gtagcggtcg
     6121 ggcacgaact ggctgcgcac cgcgtagttg tagtactcct cgtcgagcac gttgttgacc
     6181 gcggccgtca gcgtcaacgg cccctccagg aagtcgaggc gcaggtccag caccgagtag
     6241 gccgggatct tgacgccggt gttgccctcg tcgttctcca tgaactggct ggagacgtgc
     6301 cgcgcggccc cggtcagccg ccacgcgggc gtcatttgcc agacggcctg cgcggtcact
     6361 tgatgctcgg gcaccagggg aaccgtcttg ccggcgatgt ccacgttctg ctgcgtgatg
     6421 gcgctgccgg gcagcgtccc ctcgcgaaac caggcatcgg tgtaggtgta ggccgcctgc
     6481 gcggtgagcg actgcgtcgg ccgccagcgc gcctccagct ccagcccgcg ccggcgtgtt
     6541 ggcggcagat tggtgttgcc cacgccggtg ctgtagggat cgaggtggat ctcgtcgtcg
     6601 acgtcgagtt ggaacacggt cgcgcgggcg aacagcgtcg ggtcggcata ttcgacgccg
     6661 aggtcgtagc ccatcgaggt ctgcgggctc aggaactgga actcgcgcgt gaacagggcc
     6721 gaagtctcgt agatctcgtc cacggtggcg aagcggaaac ccctgccggc gcgcgcgctc
     6781 aacgcgaacg cgggcgacag ggcgtggcgc agcgcgagct cccaggcctg ctcactttcc
     6841 gtctgcgtac cggccatggc gcccgagccg aacgccgccc ccggtgcggt gggatcgaac
     6901 acgtcctgcg cgtcgatgct ctgccgctcg tagcgccagc cgccgctggc gctggtcgcc
     6961 gcggtgagct gcgcggtcag gcgcaggtaa acggcgcggt tctcctggtc ggcgctcact
     7021 cggttgatcg gctgcccgat gttggtctgc gcattgctgg tgcgcagcgc gtagtcccac
     7081 gtgtacacgt cgacgcccgc caccagcgcg gcacgcgcgc cgcccgcgcc gaacggcacc
     7141 cgcagccgcg gggtgaagga ccacacgtcc aggtccacct cgcggtagtc gggaaagccg
     7201 ccgaaatcga aataggacgt ctgcgccttg tcgcgatagc ccagccccag gttcagatcg
     7261 aaaccgggcg cttgatgctc gagctccacc gccgcgcgcc ggccatcgcg cgtggcgaag
     7321 tcgagcggcg tcgaggtgcc gcgccgatcg ctttcgagct catcgattcc cgcgctcggc
     7381 tgcaccaggc gcgcaccggg caagcgcaag tcctcggagt cggcgcccgc cttgaacgac
     7441 acggtccagc gctctggcat ccagcgcgca tccaggtagg cgctgtcggt ctcgttgcgg
     7501 ttgttgtcgc ggtacccgtc cgattcgtag tcgtagaccc cgagggccag accgacgctt
     7561 tgccccgcga agttgccgcc gacctgggct tcccgggttt cgaagctgcc gaggcgggtg
     7621 cgcaggttcg cgccgtactg cgccgcagcg gggctgcgcg tgatgatgtt gatcaccccg
     7681 gtggtggcgc cgtcaccgta ctgcaccgcg ccgctgccgc gcacgatttc gatgcgctcg
     7741 atcgccgaca gcggaaccga ggaccattgc acgccggcaa ggtcgatgtc gttgagccgc
     7801 cgcccgtcca ccaggatcaa ggtgttttgc ccggcggccg cgccgaaccc gcgcaggtcc
     7861 acggccgcgc cggccgcgct gttgccgtac aggtcgcgcg tgtggattcc ggcctccagg
     7921 accaggagtt cggggaggtt ggttgcctgg ctgcgggcga tctgctccgc gtcgatcacc
     7981 gtcacgttga ccgcgccctc ggccgccgac tcggggaagc gcgccgcggt gatcaccgtg
     8041 gcgggtgtgg tgacttcgac ggggtcggat tgcgcaagaa gatgcccgca gcacaccgcc
     8101 agcagggcgg ggccgagcct ggaaacgtcc atgttgcagc ctcccaaacg gcacgcccct
     8161 cgcgcgccgg caatcgacag ggaaaaggga ggctccggcc acggccatcg aatcgcgcac
     8221 cgcccgccgc ggagctcccg ctcgcgttgc gaggccggtc tccgggctcg cgagaacttg
     8281 gcgcgccgcc ttcccgtgct cggcgctcga tggcgcttgg cacagtggca cgatggcgcg
     8341 cccacgctcg cataccgttg cgggggcagc gcaggctttg ccgccgatcc cacccgtccg
     8401 ccccccggcg ccacctgccc ctcaagccgg ggcgatgcgc gtcgttatgg cgttcggatg
     8461 agcaggcgtg tgcttacctg cttcccgttt gacccgcggc gaagaactcg ccgcgggcac
     8521 ctcggaacgg gtccattcta gaggataagg gtcagcgctc gctcacgcgg tcgggccggg
     8581 tcgaagggat cgagtgcaca tgccgcctac attgtgtcaa acgacatgca gaagacccgg
     8641 ttagaggttg actgcatgga attccggtca ctatagtgtg acgatggaag cggagctcaa
     8701 ttccctcgac gagaagatct cccagctcat acagctatgc caccgtctac gcacagacaa
     8761 cagcgagtta cgccagcaac tggccgcggc gcagaatcaa agcaagcagc tgacggagaa
     8821 gatcgaagcc gcgcgccgac gcctggagag cctgctcagc agaatccccg aagacgcggc
     8881 gtgacgcgcg aggcgcgcgc gatcgaagtg agcatcctcg atcgcagctt ccgcatcgcc
     8941 tgcagcgagg aagaggagcc gggcttgcgc caggcggtgg agtacgtcga ccgcaagatg
     9001 cgcgagatcc gcgacgccag caagacagcc ggccacgagc gcatcgccgt catggccgcg
     9061 ctcaacatcg cccacgagct gctgtcgctg cgcgtcggcg gcggctttga catggggcag
     9121 tttaagcgta gaatgcacgc gatggctgcg gccatcgacg aggcgatgtc gacgcaggac
     9181 gagttgttct agcgcgagat cgtcgcaacg accgatgctc cctgcggtgt tcgtgcaggc
     9241 cgtacagtct gtgaaccaat gtcgtaaact tggttgcggc ctaaagtagt gctgttgtgc
     9301 gcgtcccaac gcggaagcgc ctgatgcgct cggaaagcga ccacccttga accttcggtt
     9361 caagatgaag gcctgagcgg gtgcctgcag ggggcgccca tcccgacgag cggccagcgc
     9421 cgctcgtttt gttttgcggg gcccgatcgc gtgcgctact ggttgatgaa atccgagccc
     9481 gacgtgttcg gcgtcgacga cctggcggcg gcgccgaaga agaccaccgc gtggtggggg
     9541 gtgcgcaact accaggcgcg caatttcatg cgcgacgaca tgcgcctcgg cgatctcgcc
     9601 ttcttctacc actcgagctg cccgcagccc ggcatcgccg ggatcatgcg cgtatcgcgg
     9661 ctcgcctatc ccgatgagac gcagttcgat ccgagaagcg aatatcacga cccgggatcg
     9721 gaccgggacg atccgcgctg ggtgaacgtg gacgtcacgc tggtgcgcaa gatccggctg
     9781 ctgccgatcc aggagctgcg cgcgcaccgc gcgctcgccg gcatgcgcgt gctccagcgc
     9841 ggcaaccggc tgtcgatcac gccggtgacg gcgcgcgaat gggagttcat cgagcggttg
     9901 ctctagcccg cgccgtagcg ctcgcgcagc acgctcttct gcaccttgcc catggcgttg
     9961 cgcggcagat ccggcaccag gatcacgcgc ttgggcactt tgtagttggc cagctcgcgc
    10021 ttcagcgtct ggatgatgtc ctcgggagac aagacggcgc cggcgcgcgg gaccaccacc
    10081 gcggtcaccg cctcgccgaa gtcggggtga ggcaacccca ccaccgccga ttcgagcaca
    10141 ccgggcagcg cgtcgatggc cagctcgatc tccttcggat agacgttgta gcccccggtg
    10201 atgatcaagt ccttctcgcg cccgacgatg gacaggtagc cgtccgcgtc gaacacgccc
    10261 acgtcgccgg tgcgaaacca gccgtcggcg ctgaactcct cgcgcgtctt ctccggcatg
    10321 cgccagtagc ctcgaaacac gttctcgccg cggatctgga tgccgcccac ccggcccgcg
    10381 ggcagcggcc ggtcgcgctc gtcggccacg cgcactgaca cgccgggcag aggcgggccg
    10441 acgctgcctg cccggcgctc gccatgcagc ggattggagc agatcatgcc ggcctccgac
    10501 atgccatagc gctcgaggat gcgctggccg gtgcggcgct cgaactcctc gaaggtgtcc
    10561 ggcagcagcg gcgcggaccc ggacacgaac aaccgcacgc cgccgcacgc ctgcgccgtg
    10621 aatcccggct ccgccagcaa gcgcgtgtag aaggtcggca cacccatcag gaccgtcgcg
    10681 cgcggcagat cgcgcagcag cgcccgcgcg tcgaacttgc gatgccacag catccgtgca
    10741 ccggcgagca gcgcgcaatg attggccacg aacaggccgt gcacgtggaa catcggcagc
    10801 gcatgcagca gcacgtcgcc ggcggtgaaa ccccaggcct gcaccagtac ccgcccgttc
    10861 gacagcaggt tgcggtgggt gagcatcgcg cccttggaac ggccggtggt gccggaggtg
    10921 tagatgatgg ccgccagatc gtccgccgcg acctgcgccg tctcgaagac cacgggctgg
    10981 tcgcgggccg ctccggccag ggaaccggct cccgcttcgt cgagggtcag cacttcggcg
    11041 gcgccggcga ggccgcgcac ctcggcttcg ctctgcggac ggcacaccac cacgcgcggc
    11101 tgcgcatcgc tcaggaaata gctcagctct gcgcgctggt aggcggtgtt gagcggcaga
    11161 tacaccacgc cggcacgcaa gcaggcgaga tagagaaaca gcgactgcgg ggatttctcc
    11221 acctgcgccg ccacccggtc gccgcgctgc aggccgagcg accgcaacaa cgcggcgaac
    11281 cgcgcggtct gttcctgcag cgcgcgccag ctcatttccg ccccgctgtc gaacagcacg
    11341 gtgtcgccga gatcggcggg cgccgctcgt tcgatatggg cgtagaggtt ggagttcacg
    11401 agtcgctcag ccgccgtgtg ctcgaaccct ggctcccggc gcggagcgca tcgccggctt
    11461 cggcgaagtg ctggaggcca tccagcagcg cgtcggccac tcccgtctgt atccagtccc
    11521 gcggcgacgc ggcggtgctc gcttcgtcgc cgaggatctg acggacagcg gctgggggca
    11581 gaatggcgat gcgcgccgcc ggcaaggcgc aaacgcgatc gaccggcgcg gcgaacgcca
    11641 cgtacgaagc gcccgacgca gcgcccggca gccacagcac ggttcgcggc gcgctcctcg
    11701 cccgcgcagc gccacacaag ctgaggtgca ccaggtagtc ggccagcagc acgcgttcgt
    11761 tctccaccgt ggccgcatgc gcttccgcgt cgaggaccag caccaggggg ctgccggggt
    11821 ggctatcgcc gagcgctagg agccagtcgg cgaggtgcca gcagtcggcg gcgtccaccg
    11881 gcgcgccgac gctgccgagg aacaccgcct tgcccgagcg ctcggccggc tgcgccaaga
    11941 agacgctgcc ggcgatcgtg ggaacatagc ccgtcggcaa tacgcgcgcg agcgcggcgc
    12001 tgtcggtcgc ctgcgctgac gcatgtcgca gcatgccggc gtcggccaga cgcgcgcgca
    12061 ggcgcgcatg ctcgccggcg agcaccagcg ccggccgctc gccttccacg gcgcgcgcag
    12121 ccgcgtcgcg catcgccgca ggggcgtcgt ccgcgatcgc gtcccccggg tgcagcggga
    12181 cgcgcgcctg ggcgccgatc agcgcctgca ccgcgctgcg gtccgcggcg tccagccgat
    12241 ccttgccgct tgcgctctcg atcagcgccg gtccggaggt ggcgatgcgc gtctgcgcaa
    12301 gataaatgcg gcggtcgcac aggcatgcga gcaggctggc gccgccgaag cagatcggcc
    12361 cgagcaaggc gagcagcggt gttcccgcga gcttggcctg cagcgcctcg cggaacagcc
    12421 ggcggaacgc gcccagcgcg cgcaggcctt cgtcgacgcg cgcgccggcc gagtcgagca
    12481 ccagcaaggc gggcgccgtg tgctcgcggg catcgcgcag cacggctgcc agcgcatcgg
    12541 cctccgcaac gccgattgcg ccgcgcgcga tcgccgggtc cgttgccgct acatacaccc
    12601 gccggccccg cagcacgccg ctcgcggtca gcatggcgcc ggcgctgcgt ggctgcatca
    12661 cgttgccgtc gagcagcgcc gcgacgcgct gctccgggct cagctcgctg aagcgcatgg
    12721 cgctgccatc aagcggcgag gcaccagcgg cggatgatct cctggtaggc gccgactgcc
    12781 tggtggatgc ggtcgatcac gcaatactcg tcggtctggt gcgccatctg cggctcgccg
    12841 ggccccagga tcacggtggg cggcgcgcga taggcctggc tcagcgcggc ggcgtcggtg
    12901 aaataggtcg cgctgcgcgc gaccggtcgc gcgcccagca gcggggccat cacctcgaac
    12961 acgctctgca cccagggctg ctcgggatcg gtatgcaccg cctgcacgtc catcatcggc
    13021 tccagctcga cctctgggcc gagttgccgc gccaggtccg cgagcagcgc acggtggtcg
    13081 acggtcggca cggtccggat gtcgatgccg atggtggcct ggtcgggcac cgagttgatg
    13141 ttgagcccgc cctggatggt accgacgttg agcgtggcct gacccatcag cgggtgcggt
    13201 gcattggaga agcggaacgt ctccagcttg ccgatcgccc gcgccgcctt gtacaccgcg
    13261 ttgtccccct tctcgggcat ggagccgtgc gcggtgaccc cgcgcgtgcg cgcccgcagc
    13321 cagaacgcac ccttgtgtcc gacgaaggga taattggagg tcggttcggc caccaccacg
    13381 gcgcccgcgc gccccagcgc gccgtcgtgc agcaggtgga aggcgccttc gcatccggtt
    13441 tcctcgcccg ccgtgatcac cagcgtcacg ccgggcgtgc gctccaggtg cggcgccatc
    13501 gtcacggcgg cggcgacgca ggcggccacg ccgctcttca tgtcgctgct gccgcgcccg
    13561 tacaggcggc ccgcgtcggt ctcgcccgcg aacgcatcct tcctccacgc cgccgcgccg
    13621 agcggcacga catcgacgtg cccggtcaaa cacagcggcg ggcgctcggc gccaccgatg
    13681 tcggccagca gattgacgcg gccggggccg aagctgtgat agcggatgcg gtagcccgcg
    13741 ttctcgagca atcggcccag cacgcgcgcg caggcctcct cgttgcccgg cgggttgacg
    13801 gtgttgatgc gcaggatgtc ctgcgtcagg cgtacggcat cgagggtggc ggtcatgaaa
    13861 cgagctccag cgcagcgtcg gtcgacacca gcttagccga agtaggcgcg ccgcacctcg
    13921 ggatcgctgc gcagcgcctc ggcactgccc tccgcgacca cacggccctg cgacagcacg
    13981 tagccgcgat gggcgatgga caaggtctgc ttcacgttct gctccaccag caggatcgtc
    14041 acgccggcct ggttgacacg ctggatcacc gcgaagttct ccttcaccag cagcggcgac
    14101 agccccaggg agggctcgtc gacgatcagc agccggggat cggccatcaa gccgcgcccg
    14161 atcgacagca tcgcctgctc gccgcccgac aaggtgccgg cgagctgggc ggcgcgctcg
    14221 cgcaggcgcg ggaacccagc gtacacctgc tcgattcgcg ccgctcgcgc gcgcgcgtcg
    14281 cgttcccggt aggcgccggc gaggaggttc tccaacaccg tcattttcgg gaacaccttg
    14341 cgcccctcgg ggatgcaggc gatccctcgc gccaccacct cgtgcgtcgg ccggccggcc
    14401 aggtcctcgc cgccgaacaa aatgcgcccg cggcgcgcgg gcgtgaggcc gaggatcgag
    14461 cggatcagcg tgctcttgcc ggcgccgttg gaacccagga tgcaagtgat ctcgccggga
    14521 cgcgcgcgca gcgacacgcc gcacagaacg tcggcgcggc cgtaggcggt gtacaggtcc
    14581 tcgacgacca gctcgctcat gtgcgctcaa atccccctct ccccagcagg cgaagaagtc
    14641 cctctcgcct cgcgggagag ggtagggaga ggatcggggt gagggggaaa gaacaggtat
    14701 ttctcaactg ctacccccga gatacgcttc ctgcacctgc ggatcggcaa ccacggcggc
    14761 gaacggccct tcgcagatct tctcgccgaa gttgagcacc acgcagcgct cgctgacgcg
    14821 ctcgatcacg cccatctcat gctcgatcag aatgatggac aggccgggca ggcgttcgcg
    14881 cgcagcgagg atgtcgtcca tcagctcgcg cgtctcgtcg tgcgtcatgc cggcggatgg
    14941 ttcgtcgagc aggagcagcc gcggctcgct gatcagcgcg cggcacactt ccacgcgccg
    15001 ccggtcgatc atgccgagcg caccggcggg ttcgaacatg cgctccggca attgcggatg
    15061 aaacgcggcc agcagggtcc tggcccgctc cacgtggcgc tgcagctcct gcgcgaagcg
    15121 gctgcggcgg aacagattga accacagccc cagcgacagc cgccggtggt tgccgatcat
    15181 cagattgtcg aacacggaca gcggcaggca cagccgcgag cgctggaagg tgcgcgcgat
    15241 gcccgcgtca tagatggcct gcggccgcaa gccggtgatc tcggcgccgg cgaaccacgc
    15301 gcgcccgctg gcggcgcgat acaggccggt gagggcgttg aagaaggtcg tcttgcccga
    15361 gccgttgggg ccgatcagac ccaggatctc gccttcgtgc agccgcagat cgaggccgtt
    15421 caaggcggtg aggccgccga agcgcacgct caggccctcg cagcgcagca gcgacgcggc
    15481 ttcgttcacg cccgccccca gccgggcagg tattgccgca gcttgcgcgg cagcagcccc
    15541 tcgggccgga acagcagcac caggatgacg aaggccgaga acagcaggaa gcggtactcc
    15601 tggatcacct gtagtttctc gggcagcatc accaccagcg ccgcggcggc gagaacgccc
    15661 accgggttgc ccaggccgcc gaggatgacg atcgacacca ggatcagcga ctcggccagc
    15721 gtgaagctgg cgggcgcaac gaagccggtc atcatggcgt acaccgcgcc cgctgtgccc
    15781 gccagcacgt tgccgagcgt gaacgccagg atcttccagc gcgccacctc gaagccgaac
    15841 accgaggccg cggtctcgtc gatgcgcact gcgtccaggc tcaagccgag ccaggagcgc
    15901 tccagccggc gcaccaggag cagcgcgccc gcgagcagca gcaagctgaa caccgcgtag
    15961 tcgagataga acgacaactc ggtgtactcg cccagcgcga tgttgtcgtt gaaggaccag
    16021 cccagcatgc gtagccccgg gaccttcagt ccctggggcc cgccgagggc gtcgttgacc
    16081 tcgaggaagg tcttgaacag gatggcgaag gcgatcgtga tcaaagcggc gtagtgaccg
    16141 cgggtgcgca gcagcggcaa caccagcagc gagccgatcg cgccggcgag cacgccgccc
    16201 gccgcgacca ccagcagatg cggcagcgcg gtgtgggtag cgagcaccgc tgcggtgtat
    16261 ccgccgatgc cgaagaacgc tgcgcccgag aagttgacca cacccgcgta accgaactgg
    16321 atggtgagcc ctaggcaggc gacgccgtac agcagcacgg tggcgatcat cagcagcgcg
    16381 aagtgatctt cggcgaacag cgcgacgacg accgacgtcg ccagcagcgc accccaggcc
    16441 agcggagccg ggcgaacgct ccaggcagcg cccaccgcgg cgcccaggcg cgtgcggcca
    16501 agcgcgagca gcgccaccag cgccccggcg gtgagcgcca ccacctccca ctgcgcctcg
    16561 acgcggagga acagataggc gaaacccgct atcgccgcgg tggttgcaag cgccggccac
    16621 agagcggcgg gttgcacagc gtgggttgtc atacgcgctc ggccgactgc tcggcgatca
    16681 ggccggtggg ccgccaggtc atcaccagga tcaccactgc gaacgcgaac acgtccttgt
    16741 aggcgggcac cgcgaaccac accgcgccca gcgtctgcag ccccgcgaac aggaacccgc
    16801 cgaggatcgc gccgtagagg ttgccgagcc cgcccaccac ggccgcgctg aagccgatca
    16861 cgccgagcag caaacccatg ccgaagttga tctcgttgta gtacaggccg gcggtgaccc
    16921 cggccagcgc cgcgagcgcc gagccgatcg cgaaggtgac gagcacgacg cgctggaagt
    16981 tcatgcccat gacctgcgcc gtctcgctgt cctgcgccac cgcacggatc gccaggccga
    17041 gtcgggtgcg cgtcaccagc aagtgcacgg cgacgatcga cgccaggccg agcgcgatca
    17101 ggatggcgct gtcgaagcgc agcgtgagcg cgccgaagct gacggcatcg gtcggcagga
    17161 gccgcggaaa cggcttggga ttcgcgccag cggggtagaa cagccgtatc gcctcgcgca
    17221 gcacggtgcc ggccatcagc gtgatcagca gcacattgat cggcggcgaa ccgcgcagcg
    17281 gcagcaccag ccagcgcgcc atcgccgccc cgagcagtgc gatcagcggc acggcagtga
    17341 gcatcacgcc caggctcagc agcgcattgg attccacgcc cagcgccgac agccccagat
    17401 aggcgaccag cccggtgaag gcgccgacca tgagcacgtc gccgtgcgag aacttgatca
    17461 cgtccagcac gccgaagaac agtgtgaagc ccaccgccac catcgcgtag atggtgccga
    17521 gcatgaggcc gttgaagacg tactggccga gcaggctcac gtccatcgtc ggtcccggcg
    17581 cgcgtcgggg cgcgggtacg cggtcacggc tggaccagct tgcgcttgcc cttggcgtag
    17641 tcgctgtctt cccagacgat ccacctgccg tcctgcacca cgaacttggt gatcagcggc
    17701 acggcgttct ggcgatgatc gtcgaaggtg atggtgccga ccacggtttg gtagtcgcgg
    17761 gtgcggttca gctcgctgcg caccttcttg cggtcgggtc cgaccttctc gatggcgtcc
    17821 atcaccaggt tcgccgcggc gaaggcaaac gcgccgtagg cctcgggcgg gtcgttgtac
    17881 ttctgcgcgt tgtaccgctc gaggaagaac ttgccgccgg gcagcttctc gatcggcgcg
    17941 ccttctctga acgccaggct gccctcggcc agcggcccca ggccctcgat gtacgcgtcc
    18001 gacacgatac ccgaggtgcc ctggaacacc gccttcacgc ccagcttgtc catctgcgtg
    18061 cggatgcgca cgccgagcgg cgtgatgccg ccgaagtaca cgacctcggg cttgaggtcc
    18121 ttgatcttgg tgagctcggc ggagaagtcc tgctgatcgg cagtcacgcc gaaggtgccg
    18181 aggatgcggc cgccgttctt ggcgagatac tcgctgaagt acttgttgtg ccccttgccg
    18241 taatcggtgg tgtcgtggat caccgcccag cgccgatact tgagttcggt catgaagcgg
    18301 gccgcggtct cgttctgatt gatcatggtg ccgttgacgc ggtgcacttc cgggtagtcg
    18361 ttggcatagg tgatgtcggg cagcaccgca ccccacacca ccaccggcag cccgaacttg
    18421 tgatacacgt ccaccgtggc catcgccacc gtcgagcaat agtgggtgac gccggcgacg
    18481 atgctgcggt cggcggccgc cttggtggcg acctgcacgc cgatgttggg cttgcactcg
    18541 tcatccaggg acaccagttc gtaccggtac ctggccttgg cttcggcgtt gcgcagcctg
    18601 accgccaggt cggtggcatt gcggccgcca aggccgttcg ccgacacgcc gccggtgagc
    18661 ggcccgatga acaccagctt caccgcctcc ttcgcgccgg cggcgccggc ggcgagcagc
    18721 gcagcacagc acagggcggc acggagcggt cgcggcaaca tcttcaggtc tcctggtccg
    18781 gattattggt gagaagggcg gcgggtctgc cgggtcggtc ccggagggct gaggctatgg
    18841 gctcggccgc gccttgtcaa ttcgccgcag cgcaggcgtg gtcggctcgc cgcggcgcgg
    18901 ccgggcctgg cgaaaaaaaa agcggcgcaa gccgcttttc ctcgagcccc ggcgggtgcc
    18961 ggggaattat tgttgttcgt cgttgttgtc gttatttctt gcgcaggcgc cggatcgccg
    19021 cgagctgcgc ggcgagcatc gccagctcgc cttccaccac ggcgagttct tccttgccct
    19081 tggcgttgcg gcgcgcctcc tcggcgcgct gctgggcttc gatcgccttg gcctcgtcca
    19141 gatcgtgacc gcggatggcg gtgtcggcga gcaccgtgat caggttcggc tgcacttcca
    19201 gaatcccgcc ggcgacgaag atcagctcct cctcgtcctt gccggcggcc ttgatgcgca
    19261 ccgagcccgg cttgatgcgc gtgatcaacg gggtgtgacg cggatagatg cccagctcgc
    19321 cggcctcgcc gggcaggacc acgaactcgg cctcgcccga gtagatgccc tcctcggcgc
    19381 tgaccacatc gatgtgaatc gtgttcgcca tgctggtctc tattacctca cacaccacaa
    19441 cgcgccggcg tctgcggcgc caccccgcct cgtgagaggt gaggagcggt tttaaagtcg
    19501 cgccttaagg gcgcctataa cgtcttcgcc ttctcgatcg cctcctcgat cgggccgacc
    19561 atgtagaacg cctgctcggg caggtggtcg cactcgccgt tgacgatcat cttgaagccc
    19621 ttgatggttt ctttgagcgg cacgaacttg cccgcggtgc cggtgaacac ttcggccacg
    19681 tggaacggct gcgacaggaa gcgctggatc ttgcgggcac gcgccacggt gagcttgtcc
    19741 tcgggggaca gctcgtccat gcccaggatg gcgatgatat cgcgcagctc tttgtagcgc
    19801 tgcagggtgg cctgcacctg gcgcgtggtg ttgtagtgct cctcgccgat gacgttgggg
    19861 tcgacctggc gcgaggtcga gtcgagcggg tccaccgcgg ggtagatccc cagcgatgca
    19921 atgtcccgcg acagcaccac ggtggcgtcc aggtgcgcga aggtggtggc gggcgagggg
    19981 tcggtcaggt cgtccgccgg tacgtagatc gcctgcaccg aggtgatcga gccggtcttg
    20041 gtggacacga tgcgctcctg caggcggccc atttcctcgg ccagcgtcgg ctggtagccc
    20101 accgccgaag gcatccggcc gagcagggca gacacttccg tcccggccag ggtgtagcgg
    20161 tagatgttgt cgatgaacag caggatatcg cggccgtcat cgcggaagcg ctccgccatg
    20221 gtcagcccgc tcaggcccac gcgcaggcgg ttgccgggcg gctcgttcat ctgcccgaac
    20281 accatcgcca ccttgtcgag cacgttcgac tccttcatct cgtgatagaa gtcgttgccc
    20341 tcgcgagtgc gttcgcctac gccggcgaac accgacagcc cggcgtgctg cttggcgatg
    20401 ttgttgatca gctccatcat attcacggtc ttgcccacgc ctgcgccgcc gaacaggccg
    20461 atcttgccgc ccttggcgaa cgggcagacc aagtcgatga ccttaatgcc ggtttcgagc
    20521 agctcgacgg aaggggacag ctcgtcgaac ttgggcgccg gctgatggat cgcgcggcgt
    20581 tggtcggcct ggatcggccc ggcctcgtcg atcggccggc cgagcacgtc catgatgcgg
    20641 cccagcgtgc ccggtcccac cggcaccgag atcggtgccc cggtgccgcg caccttcatg
    20701 ccgcggcgca gcccatccga ggaaccgagc gcaatgcagc gcaccacccc gtcgccgagc
    20761 tgttgctcga cctcgaaggt gaggcccttc tcggcgaagg atgcggcgtc gccgtcctcg
    20821 agcagcagtg cgtcgtaaac cttgggcatg ccctcgcgcg gaaactcgat gtcgatgacc
    20881 gcgccgatgg tctggacgat ggtgccttgt gcgtttgcca tgaatgttcc tggattctgg
    20941 atcgggatta tccggtcaga cggcggccgc cccgccgacg atctccgaca gctccttggt
    21001 gatgccggcc tgccggttct tgttgtagat cagggtgagc tcgtcgatca gggtttcggc
    21061 attgtccgag gcggccttca tcgccaccat gcgcgccgac tgctcggagg ccatgttctc
    21121 cgccaccgcc tggtagatca gcgcctcgat atagcgcgtc aacacctgat cgagcaccgc
    21181 cttggcctcg ggctcgtaga tgtagtccca gctgccctcg ggtgccccca gtcggtcgcc
    21241 ggcgagcggc agcagctgct ccatcaccgg ctcctgcttc atggtgttga tgaagcgcgt
    21301 gtagaacatc agcaggcggt cgaagcgatc gctggcgtag ccgtcgagca tcaccttcag
    21361 ggcgccaatc agccgctcca tgtgcgggcg gtcgcccagc gcggtgacct gcgagacgat
    21421 gttggcgccg accc
//
LOCUS       DASPGE010000233         4948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_530560, whole genome shotgun sequence.
ACCESSION   DASPGE010000233 DASPGE010000000
VERSION     DASPGE010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530560"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..3729
                     /gene="metH"
                     /locus_tag="VD791_07500"
     CDS             10..3729
                     /gene="metH"
                     /locus_tag="VD791_07500"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HXV07857.1"
                     /translation="MSKDRLHLLHQALAQRILILDGAMGTMIQSHRLEPHQYRGDLFR
                     DHACDQKGNNDLLTLTRPDLIRAIHAGYLEAGADILETNTFNSTRISMADYGMEDAAH
                     RLNRQGAQLARAVADGFTRRTPHRPRFVAGALGPTNRTCSISPDVNDPGARNVSFDEL
                     VAVYSEAVDGLVTGGVDLLLIETIFDTLNAKAAIFAIEDWFERHGAWLPVIVSGTITD
                     RSGRTLSGQTVGAFWNAVAHAKPLAIGLNCALGAKDLRPHIEELAAIADTHTSLYPNA
                     GLPNEMGEYDESPEYMAAILGEFARAGFLNLVGGCCGTTPVHIRAIAEAVEGCAPRTV
                     PKVEKKLRLSGLEPLDIGDDSLFVNVGERTNVTGSKAFARLVLAGNYADGLKVARQQV
                     ENGAQMIDVNMDEAMLDSKQAMTTFLKLIASEPDISRVPVMVDSSKWSVIEAGLKCLQ
                     GKPVVNSISLKEGEAEFLRQAKLVRRYGAAAVVMAFDEQGQADTLARRIEICTRAYHL
                     LVKEAGFPPEDIIFDPNIFAVATGLEEHNRYALDYLEATRAIKTALPHAKVSGGVSNI
                     SFSFRGNEPVRAAIHTAFLYHAIQAGMTMGIVNAGQLGVYEEIPKDLLERVEDVLFNR
                     RPDATERLVAFAETYKCEGKQAVEDLSWRQGTVQERLSHALVKGIDTWVVEDTEEARL
                     QYQRPIEVIEGPLMDGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAHLVPFIEAEKAR
                     TGDAQRPKGRIVLATVKGDVHDIGKNIVGVVLQCNNFEVVNLGVMVPAEKILQTAREQ
                     QADLIGLSGLITPSLEEMVHVAKEMQRQCLELPLLIGGATTSRVHTAVKIEPNYQAPV
                     VWVPDASRSVGVCTSLVSSGLKDEFVRKTRAEYERVREQHKSKTGQAPHHILAEARRH
                     GLKTDWASYRPPEPRAPGIAVFREYPLDEIVPFIDWTPFFQTWELSGRYPRILQDKVV
                     GEAARNLFADAQAMLERIVQERWLQASGVVGLFPANSVGDDVEVYADESRTQAIATFH
                     FLRQQAVKPEDRANHCLADLIAPRASGVRDYLGAFAVTAGLGIEPRLEAYEAKHDDYS
                     AIMLKALADRLAEAFAELMHLKVRRELWGYAAAENLDAEALIDEKYRGIRPAPGYPAC
                     PDHTEKGPLFDLLDARDNADIILTESFAMLPTAAVSGFYFSHPQSQYFAVGKIDRDQV
                     EDYARRRGWDLETAERWLAPNLAYEPAARREPVEV"
     gene            3887..>4948
                     /locus_tag="VD791_07505"
     CDS             3887..>4948
                     /locus_tag="VD791_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HXV07858.1"
                     /translation="MPTYDAIVIGSGLGGLTAAAVWARQGKRVLVLERQSRFGGAAKV
                     YSHGPLAIEASLHELDGLDPDDAKTDLLRDLGVWDKLHFLDVGALYEVRGARLGTPFL
                     LPRGIESAREAALWRFPHSERGIHQFFHALRSVQAAFRSVDRPRELGWWLRHGADVAA
                     NILPVIANARHSVASFLDAALGEDEAAKIALAANLGYLDNDPARLWFLVFAMVQASYL
                     TGGGHYLQGGSQSLTDALLTVIRESGGETLAERDATAILLDDAGRVAGVAHRGSDGAA
                     DVAHAPVVFGNAAPHVLAGLLPAGARQRLVDTFSHRALSTSLFVVSLGMQRRPSDFGV
                     RTYSTFLYPDWLSSLREMRD"
BASE COUNT          821 a         1670 c         1702 g          755 t
ORIGIN      
        1 gtgatcgaaa tgtcaaagga ccggctgcac ctgctgcacc aggcgctcgc gcagcgcatc
       61 ctgatcctgg acggggccat gggcaccatg atccagagcc accgcctgga gccgcatcag
      121 tatcgcggcg acctgttccg cgatcacgcc tgcgatcaga agggcaacaa cgacctgctc
      181 acgctgacgc ggccggacct gatccgcgcc atccacgccg gttatctgga agccggcgcc
      241 gacatcctcg agaccaacac cttcaactcc acgcgcatct ccatggccga ctacggcatg
      301 gaggacgcgg cgcaccggct gaaccggcag ggcgcgcagc tggcgcgcgc agtcgccgac
      361 gggttcaccc gccgcacgcc gcaccgcccg cgtttcgtgg ccggcgcgct gggccccacc
      421 aaccgcacct gctcgatctc gccggatgtg aacgatccgg gcgcgcgcaa cgtcagcttc
      481 gacgagctgg tcgccgtgta ctcggaagcg gtggacgggc tggtcacggg cggcgtcgat
      541 ctgctgctga tcgagacgat cttcgacacg ctcaacgcca aggccgcgat cttcgccatc
      601 gaggactggt tcgagcggca cggcgcctgg ctgccggtga tcgtctcggg cacgatcacc
      661 gaccgctccg gccgcacgct ctcgggtcaa accgtgggcg ccttctggaa cgcggtggcg
      721 cacgccaagc cgttggcgat cgggctgaac tgtgccctcg gcgcgaagga cctgcgtccg
      781 cacatcgaag agctggcggc tatcgccgac acccacacct cgctctaccc caacgcgggg
      841 ctgcccaacg agatgggcga gtacgacgag tcgcccgagt acatggccgc gatcctgggc
      901 gagttcgccc gcgccggttt cctgaatctg gtgggcggct gttgtggcac cacgccggtg
      961 catatccgcg ccatcgccga ggcggtcgag gggtgcgcgc cgcgcaccgt gcccaaggtc
     1021 gagaagaagc tgcgcctgtc gggcctggag ccgctcgaca tcggcgacga ttcgctgttc
     1081 gtgaacgtcg gcgagcgtac caacgtgacc ggctcgaaag ccttcgcgcg cctggtcctc
     1141 gccggcaact acgccgacgg gctgaaggtg gcgcgccagc aggtggagaa cggcgcgcag
     1201 atgatcgacg tgaacatgga cgaggcgatg ctggactcga agcaggccat gaccacgttc
     1261 ctgaagctga tcgcctcgga gccggacatc tcgcgcgtgc cggtgatggt ggactccagc
     1321 aaatggagcg tgatcgaggc cggcctcaag tgcctgcagg gcaagccggt ggtcaattcc
     1381 atcagcctga aagagggcga agccgagttt ctgcgccagg cgaagctggt gcggcgctac
     1441 ggggcggcgg ccgtggtcat ggcgttcgac gagcagggtc aggccgacac gctggcgcgc
     1501 cgcatcgaga tctgtacgcg cgcctaccac ctgctggtga aggaggccgg cttcccgccc
     1561 gaggacatca tcttcgaccc caacatcttc gcggtggcca ccgggctcga ggagcacaac
     1621 cgctacgccc tggattacct cgaagccacc cgcgcgatca agacagcgct gccgcacgcc
     1681 aaggtgagcg gcggcgtgtc gaacatctcg ttctccttcc gcggcaacga accggtgcgc
     1741 gcggccatcc acaccgcctt cctgtaccac gccatccagg ccggcatgac gatgggcatc
     1801 gtcaacgctg gtcagctcgg tgtgtacgag gagatcccga aggacctgct ggagcgcgtc
     1861 gaagacgtgc tgttcaaccg gcggcccgat gcaaccgagc gtctggtcgc cttcgccgaa
     1921 acttacaagt gcgagggcaa gcaggcggtc gaggacctgt cctggcgcca gggcacggtg
     1981 caagagcggt tgagccacgc cctggtgaaa ggcatcgaca cctgggtcgt cgaggacacc
     2041 gaggaggcgc gcctgcagta ccagcggccg atcgaggtga tcgaaggccc gctgatggac
     2101 ggcatgaacg tggtcggcga cctattcggc gccggcaaga tgttcctgcc gcaggtggtg
     2161 aagtcggcgc gcgtcatgaa gcaggcggtc gcgcacctgg tgcccttcat cgaggccgag
     2221 aaagcccgca ccggcgacgc ccagcgcccg aagggccgaa tcgtgctggc caccgtcaag
     2281 ggggacgtgc acgacatcgg caagaacatc gtcggggtgg tgctccagtg caacaacttc
     2341 gaggtggtga acctgggcgt gatggtgccc gccgagaaga ttctgcagac cgcgcgcgaa
     2401 caacaagccg acctcatcgg gctgtccggg ctgatcacgc cctcgctcga ggagatggtg
     2461 cacgtggcca aggagatgca gcgccagtgc ctggagctgc cgctgctgat cggcggggcc
     2521 accacgtccc gcgtgcatac cgcggtgaag atcgagccca actaccaggc gccggtggtg
     2581 tgggtgccgg acgcctcgcg cagcgtgggc gtgtgcacca gcctggtgag cagcgggctg
     2641 aaggacgagt tcgtgcgcaa gacccgtgcg gaatacgagc gcgtgcgcga gcagcacaag
     2701 agcaagaccg gccaggcgcc gcaccacatc ctggccgagg cgcgccggca cggcttgaag
     2761 accgactggg cgagctaccg tccaccggag ccgcgcgcgc cgggcatcgc cgtgttccgg
     2821 gagtatccgc tggacgagat cgtgcctttc atcgactgga cgccgttctt ccagacctgg
     2881 gagctgtccg gccgctatcc gcgcatcctg caggacaaag tggtgggcga ggcggcgcgc
     2941 aacctgttcg ccgacgcgca ggcgatgctc gagcgcatcg tgcaggagcg ctggctgcag
     3001 gcgagcggcg tggtggggct gttcccggcc aacagcgtgg gcgacgacgt cgaggtatat
     3061 gccgacgagt cgcgcacgca ggcgatcgcc accttccact tcctgcgcca gcaggcggtg
     3121 aagccggagg accgcgccaa tcactgcctg gccgacctga tcgcgccgcg cgcgagcgga
     3181 gtgcgcgact acctcggcgc cttcgcggtc accgcggggc tcggcatcga gccgcggctc
     3241 gaggcctacg aggcgaagca cgacgattac agcgcgatca tgctcaaggc gctggccgac
     3301 cggctggccg aggccttcgc cgagctcatg catttgaagg tgcggcgcga gctatggggc
     3361 tacgcagccg ccgagaacct ggacgccgaa gcgctgatcg acgagaagta ccgcggcatc
     3421 cggcccgcgc ccggctatcc ggcgtgtccg gaccacaccg agaaaggccc gctgttcgat
     3481 ctgctggacg cgcgcgacaa tgccgacatc atcctcaccg aaagtttcgc catgctcccc
     3541 accgccgcgg tcagcggctt ctacttttcc catccccagt cgcagtattt tgcggtcggc
     3601 aagatcgacc gcgaccaggt ggaggattac gcgcgccgcc gcggctggga cctggagacc
     3661 gccgagcgct ggcttgcgcc caacctggcc tacgagccgg cggcgcggcg cgagccggtc
     3721 gaagtctgat aacggctcgc cgaccacccg gctcgggtgc gcggcaaccg gggtggccgg
     3781 caatccggcc ggtcccgccg tcacttgacc agcgtcaagg cgagcccatt ggcagtgtcc
     3841 atactggttt gcagtaccaa gcagcgcgtg ctcgccgcta actcgcatgc caacctacga
     3901 tgccatcgtg atcggttccg gcctcggcgg actgaccgcc gcggcggtgt gggcgcggca
     3961 gggcaagcgc gtgctggtgc tcgagcggca gagccgcttc ggcggcgcgg ccaaggtcta
     4021 ttcgcacgga ccgttggcga tcgaagcttc gctgcacgag ctggatggcc tggatcccga
     4081 cgacgccaag accgacttgc tgcgcgatct gggcgtgtgg gacaagctgc acttcctcga
     4141 cgtgggcgca ctgtacgaag tgcgcggcgc acggctgggc acgccgtttc tcctccccag
     4201 gggcatcgag tccgcgcggg aagcggccct gtggcgcttt ccccacagcg aacgcgggat
     4261 ccaccagttc tttcacgctc tgcggtcggt gcaggctgcg ttccgctcgg tggaccggcc
     4321 gcgcgagctg ggctggtggc tgcgtcacgg cgcggatgtg gccgcgaata tcctgccggt
     4381 gatcgccaat gcgcgtcact ccgtcgcgag cttcctggac gcggccctgg gcgaggacga
     4441 agcggcgaag atcgctctcg ccgccaacct cggctatctc gacaacgatc cggcgcgcct
     4501 gtggttcctg gtcttcgcca tggtgcaggc gagctacctc accggcggcg ggcattattt
     4561 gcagggcggg tcgcagagtc tcaccgatgc actgctcacc gtgatccgcg aatcgggcgg
     4621 cgagacgctc gccgagcgcg atgccaccgc gatcttgctc gacgacgccg ggcgcgttgc
     4681 cggggtggcg caccgcggct cggacggcgc tgccgacgtg gctcacgcgc cggtggtgtt
     4741 cggcaacgcg gcgccgcacg tgctcgccgg gctgctgccc gccggtgcac gccaacgctt
     4801 ggtggacact ttcagccacc gcgcgctgtc cacctcgctg ttcgtcgtgt cgctcggcat
     4861 gcagcgccgg ccgtcggact tcggcgtgcg cacctacagc acattcctgt acccggattg
     4921 gctgagctcg ctgcgtgaga tgcgagac
//
LOCUS       DASPGE010000234         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_882790, whole genome shotgun sequence.
ACCESSION   DASPGE010000234 DASPGE010000000
VERSION     DASPGE010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882790"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VD791_07510"
     CDS             complement(<1..1075)
                     /locus_tag="VD791_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXV07859.1"
                     /translation="MSIDYRPEDPAFLADPFPLLRRLQDEDPCHWSPRLKAWVVTRYE
                     DVRDICLQRDMSSDRLRPFFAALPSPEAQRIADIIRYLSLWMVFRDPPEHTRLRRLTS
                     KVFHVKSMHAMRPRIEALVRWLFDAIGGRREFDFIADFAGPLPALVIMDMLGVPRDEL
                     PRVKRLSDDMALFIGSSRMAPEKYGIAQAATRAMADFFRDMIAARRATPRPDLLSELV
                     HLRDGDDRLTEDELVATCILLLFAGHETTTNHLANGLLGLMRFPQELRKLRDDPTLAP
                     AAVEELLRYDGPNGVQVRIVGRDRDMHGKTLRTGERVFIQLNAANRDPRAYADPDRVD
                     LARNGPPHLAFGFGAHICLGFPLA"
     gene            complement(1072..1953)
                     /locus_tag="VD791_07515"
     CDS             complement(1072..1953)
                     /locus_tag="VD791_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXV07860.1"
                     /translation="MVEDKDPVRGIAWIFPGLFVVLWSTGFVGAKLGLAYAEPATFLV
                     IRFLIVIALLLPLCVWMRAPWPATPAQWLHMAFAGALIQAGYLGGVFLSLHRGMPAGV
                     SSLITGLQPILAAVLSGWLLGERTNARQWFGLMLGIAGVALVVQEKWVLKDVGAVATA
                     LSVLALASISLGAVWQKRYCARVDLRTGAAIQFTAAALVLAPYAWLFETREVIWSPTL
                     VVVLAWLVFALSLGAVLLLFWLIRHGAATKVASLMYLVPPCTALLAWPLFGETYTVFS
                     AAGMGVTMLAVWLVSKG"
     gene            2185..>2542
                     /locus_tag="VD791_07520"
     CDS             2185..>2542
                     /locus_tag="VD791_07520"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HXV07861.1"
                     /translation="MYVTPEQIQAAGKANVETALSLAAMQFAAMEKLATLNANAVKSA
                     FEDSLSNARALFGAKDVQEFINLQSTLATPAIEKAIAYSKSVYEVASETNAELSKVAE
                     KGVAEWNENFSSLLDKA"
BASE COUNT          484 a          861 c          851 g          346 t
ORIGIN      
        1 gcgccagcgg gaagcccagg cagatgtggg cgccgaagcc gaatgcgagg tgcggcggcc
       61 cgttgcgggc gaggtcgacc cggtcggggt ccgcgtaggc gcgcgggtcc cggttggcag
      121 cgttcagctg gatgaatacc cgctcgccgg tcctgagcgt cttgccgtgc atgtcccggt
      181 cccggcccac gatcctgacc tgcacgccgt tcggtccgtc gtagcgcaga agctcttcca
      241 cggccgccgg cgcgagcgtg ggatcgtcgc gcaattttcg cagctcctgc ggaaagcgca
      301 tcaggccgag cagcccgttc gccaggtggt tggtggtcgt ctcgtgcccc gcgaacagca
      361 gcaggatgca ggtggcgacg agctcgtcct ccgtcagccg gtcgtccccg tcccgcaggt
      421 gcaccagctc cgagagcaga tccggtcggg gtgtggcgcg ccgtgccgca atcatgtcgc
      481 ggaagaagtc cgccatggca cgcgtcgccg cctgcgcgat gccgtatttc tccggggcca
      541 tgcgcgagct gccgatgaac agcgccatgt cgtcggacag ccgcttcacg cgcggcagct
      601 cgtcgcgcgg cacgcccagc atgtccatga tgaccagggc ggggagcggc ccggcgaagt
      661 ccgcgatgaa atcgaactcg cgacgcccgc cgatggcgtc gaacagccac cgcaccagcg
      721 cctcgatgcg cgggcgcatg gcgtgcatgg acttcacgtg gaagaccttg ctggtcagcc
      781 gccgcagccg cgtgtgctcg ggcggatcgc ggaaaaccat ccacagcgag aggtagcgga
      841 tgatgtcggc gatgcgctgc gcctccggcg acggcagggc ggcgaagaac gggcgcaggc
      901 ggtcggaaga catgtcgcgt tgcaggcaga tgtcgcgcac gtcctcgtag cgcgtcacga
      961 cccaggcctt caggcgcggg ctccagtgac aggggtcctc gtcctgcagg cggcgcagca
     1021 ggggaaacgg atcggcgagg aacgccgggt cctcggggcg gtagtcgatg ctcatccctt
     1081 gctcaccagc cagacggcca gcatggtgac ccccatcccg gcggccgaga atacggtgta
     1141 ggtctcgccg aacaacggcc aggcgaggag cgccgtgcag ggcggaacga ggtacatcag
     1201 gctcgccacc ttggtggcgg cgccgtgacg gatgagccag aagagcagga gcaccgcgcc
     1261 cagcgagagc gcgaacacca gccaggcgag gaccacgacc agcgtcgggc tccagatcac
     1321 ctcccgcgtc tcgaacagcc aggcgtaggg cgccagcacc agcgccgcgg cggtgaactg
     1381 gatcgcggcc ccggtgcgca ggtccacgcg cgcgcagtag cgcttctgcc agacggcgcc
     1441 caggctgatg ctcgccagcg cgagcacgga aagcgccgtc gccaccgcgc cgacgtcctt
     1501 gagaacccat ttctcctgca ccaccagcgc gaccccggcg atgccgagca tcaagccgaa
     1561 ccactggcgt gcgttggtcc gctcgccgag cagccagccg ctcagcacgg cagccaggat
     1621 cggctgcagc cccgtgatca gcgaggacac gcccgccggc atgccgcggt gcagggacag
     1681 gaacaccccg cccaggtaac ccgcctggat gagcgcgccc gcaaacgcca tgtgcagcca
     1741 ctgcgcaggg gtggccggcc atggcgcccg catccagaca cagagcggca gcaacagcgc
     1801 gatcacgatg aggaaccgga tgaccagaaa agtggccggc tcggcgtaag caagccctaa
     1861 ctttgcacct acgaagcctg tactccagag cacgacgaac aacccgggga agatccaggc
     1921 gatgccccgg accggatcct tgtcttcgac catggccgat tgtctccgct cggcagcgcc
     1981 gctgccggtg gatttcacgc gagtggcttg ggccgtttga aacgggcttg gcccaccgag
     2041 cggcagatct cgcagtacat caagtagtta tggtgcaccg caaaaacatc ttgacttcaa
     2101 agggcaaagg cctagaattg tctttgctgc ggcgcaccaa tttagcgccc gaccgaatct
     2161 aaaccgaccg acaaggagca ccaaatgtac gtcacccccg agcagatcca ggcggccggc
     2221 aaggccaacg tggaaaccgc gctttcgctc gccgcgatgc aattcgccgc gatggagaag
     2281 ctggcgaccc tgaacgccaa cgccgtgaag tccgcattcg aagacagcct gagcaacgcc
     2341 cgcgcgctgt tcggcgccaa ggacgtccag gaattcatca acctgcagag cacgctggcc
     2401 accccggcca tcgagaaggc gatcgcctac tccaagagcg tgtacgaagt cgcctccgag
     2461 accaacgccg agctgtccaa ggtcgccgag aagggcgtcg ccgagtggaa cgagaacttc
     2521 agctcgctgc tcgacaaggc gg
//
LOCUS       DASPGE010000235         8165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_882951, whole genome shotgun sequence.
ACCESSION   DASPGE010000235 DASPGE010000000
VERSION     DASPGE010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882951"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1217
                     /locus_tag="VD791_07525"
     CDS             81..1217
                     /locus_tag="VD791_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HXV07862.1"
                     /translation="MERDQAIKFVHELLRVMVSKKASDLFITAGFPPAFKVDGKMTPV
                     SNQSLTPTHTMEIARAIMNDKQAQEFEATKECNFAISPPGIGRFRVNAFVQQGRVGVV
                     LRTITTTIPRLEDLGLPDVLKDVAMTKRGLVIVVGATGSGKSTSLAAMLGYRNENSHG
                     HIITIEDPVEYVHDHKNCLITQREVGVDTESWHSALKNTLRQAPDVILIGEIRDRDTM
                     DYAVAFAETGHLCLATLHANSSNQALDRIINFFPEERRLQLLMDLSLNLKGMISQRLI
                     PRRDGPGRVAAIEIMLNSPLISDLIFKGAVHEIKEIMKKSRELGMQTFDQALFDLYEG
                     GLISYEDAMRNADSMNELRLAIKLHGHEAKEKDMLTGLDHLNIV"
     gene            complement(1242..2444)
                     /locus_tag="VD791_07530"
     CDS             complement(1242..2444)
                     /locus_tag="VD791_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HXV07863.1"
                     /translation="MSERPRSRTRSGPDVGRQHFFSPCPRGLEEVLRAELEALGALEL
                     DPRDGGVGFAGEFELCYAVNLHSRIASRVLWRLAHAPYRDEDDVYRVVAALPWREHFA
                     PERTIRVNVSATGSPLKSLDFVTLRIKDAVCDRFRADCGRRPSVDTANPDVRIHAYFD
                     AATLSLYLDTSGEPLFKRGLRVAAAEAPLRENLAAGILHLAGWAPGIPLVDPMCGGGT
                     FLVEAAEAALRRPPGGRRRFAFENLHNFDAARWAALRAQAQAAECPPQPLSIFGSDLY
                     GAALKLARENLSRAGLEGVVRLKQANALEISAPAAAGVLVMNPPYGVRLGEQQALADL
                     YPRLGDALKQRFAGWRACIFTADLRLPKLIGLKPARRIPLYNGALECRLYVFELVAGA
                     MREKRREA"
     gene            complement(2471..2845)
                     /locus_tag="VD791_07535"
     CDS             complement(2471..2845)
                     /locus_tag="VD791_07535"
                     /inference="COORDINATES: protein motif:HMM:NF020376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HXV07864.1"
                     /translation="MARALLAAALVLATLAAPAAEVASEIMVHSTLAAGLRHHDAKAV
                     WAQMKPGDEVTLVREIDNPHDANAVRVEWNGRVLGYLPRADNASVARQLDRGNPLRAR
                     IARLEKYRNHRLKLEIEVYLRL"
     gene            complement(2865..3281)
                     /locus_tag="VD791_07540"
     CDS             complement(2865..3281)
                     /locus_tag="VD791_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HXV07865.1"
                     /translation="MLWVKALHIVFMVTWFAGLFYLPRLFVYHAMAQDAQGIERFRVM
                     ERKLYYGIMTPGAVLTIVFGVWLWLQWFRDAGGWMYAKLALVALLIAYHLWCGKLLGD
                     FRHGRNRRSHVWYRWFNEVPVLILLAAVLLVVVKPF"
     gene            complement(3291..4076)
                     /locus_tag="VD791_07545"
     CDS             complement(3291..4076)
                     /locus_tag="VD791_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV07866.1"
                     /translation="MIAKHFAPPRISARFVQVWRRNFLVWRKLAVTSILGNLADPMLY
                     MLGLGYGLGGLLPQVDGIPYITFLAAGTVCYSTMNSATFEALYSGFSRMHVQRTWDAI
                     MNAPLNLDDIVLAETLWAASKSFLSGIAILIIIWALALSHSPLTLWIVPLTLLIGLTF
                     GTLGLTMTALAPSYDFFLYYFTLVITPMVLLCGVFFPVAQLPPLLQQVSAVLPLTHAI
                     DLVRPLVNGEIPQRALLHLGVLVGYAAAGFSAALVLTRRRLLQ"
     gene            complement(4088..4993)
                     /locus_tag="VD791_07550"
     CDS             complement(4088..4993)
                     /locus_tag="VD791_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020699604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV07867.1"
                     /translation="MLSLAGVRKSYGGQEVICGVDLDLRAGECYGLLGPNGAGKTTTL
                     RLALGLTEPDAGRIRLLGEPIPRRAREARLRLGVVPQLDNLDPDFTVLENLLVYGRYF
                     GLPDDAIAARIPRLLEFAGLEGRAQARIQALSGGMKRRLSLARALVNDPDLIFLDEPT
                     TGLDPQARHLIWERLKQLVAQGKTILLTTHFMDEAERLCDRLSVMDHGRLIITGTPQE
                     LIAHHIEPQVVEVYGEGAPAWAQAHAQRLCRRWERAGETVFCYTGDGEPVLRTLEGQR
                     DVRYLHRRANLEDVFLKLTGRDLRD"
     gene            complement(4993..5604)
                     /locus_tag="VD791_07555"
     CDS             complement(4993..5604)
                     /locus_tag="VD791_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXV07868.1"
                     /translation="MRPARDERALLPGPAGNLEIVVSDPGEARRGLALIAHPHPLYGG
                     TLDNKVVQTLARCFHAQGHVAVRMNFRGVGASDGTFDEGVGETQDWLHVARAMQGRFG
                     DLPLALAGFSFGAFVQAQVARTLAPARLVLVAPAVNRFALQQVPAGTLVIHGDEDDVV
                     PLREVLDWARPQSLPVVVMPGAGHFFHGKLVELARVVNHSCAC"
     gene            complement(5601..5909)
                     /locus_tag="VD791_07560"
     CDS             complement(5601..5909)
                     /locus_tag="VD791_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HXV07869.1"
                     /translation="MSYYRHHVFFCCNQRAAGEACCNNHGSQQMRDYAKQRVKELGLS
                     GPGKVRVNQAGCLDRCEEGPVIVVYPEEVWYTYVDRADVDEIIEEHLRHGRIVERLRL
                     "
     gene            complement(6097..6831)
                     /locus_tag="VD791_07565"
     CDS             complement(6097..6831)
                     /locus_tag="VD791_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXV07870.1"
                     /translation="MRIAILEDDISQTELLSHWLQLAGHNAHAFEQGANLLRALERES
                     FDALLLDWNVPDLSGVEVLKRVRQRLHSSVPVLFVTGRNREDDVVTALREGADDYMIK
                     PVRRLELLARLEALSRRGRHGPEQAEVIELDVFKVDCQTRTVHRNGKQVDLTAKDFDL
                     SVLFLRNVGRLLSRGHIRETVWGPNAVVTSRTLDTHVSRIRNKLGLTPEFGWRLAAVY
                     RYGYRLEHLDAANAQRIVHSDPKAAA"
     gene            complement(6887..7234)
                     /locus_tag="VD791_07570"
     CDS             complement(6887..7234)
                     /locus_tag="VD791_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HXV07871.1"
                     /translation="MTGPRRTNPTEITRDSLQPRVVWLEKDLEQLVTRFLRRKEDELA
                     RLRQALSGADFETIRKLGHDLKGAGEGFGFPELSILGAKFELAARAADTELIAVHITT
                     MERYLKRLQVRFD"
     gene            complement(7231..7617)
                     /locus_tag="VD791_07575"
     CDS             complement(7231..7617)
                     /locus_tag="VD791_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV07872.1"
                     /translation="MTQAELAVLVAEDNEDIRELVVHQLRRMGVNVLQAENGRTALDL
                     ALSRQPALVLMDMDMPVMTGFEAVAELRLRGYTKPIYALTGHGEGPEAERALSSGCDA
                     LLAKPIRSDVLRAHVQSVLARPGGVL"
     gene            complement(7863..>8165)
                     /locus_tag="VD791_07580"
     CDS             complement(7863..>8165)
                     /locus_tag="VD791_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07873.1"
                     /translation="YGELVDPHRGGVLISLPAAGAVRGMLISAFYFAPGGSEGAEQYG
                     LLINAPLEVVARAFPEFAARHTVNGHVRRLARLSEQTGDRSASRQTLLMCVAGTAV"
BASE COUNT         1377 a         2814 c         2781 g         1193 t
ORIGIN      
        1 gtgaaagcgg cgaacaagga cctgttcgca ggctgacggc gccgggtcgc actcaacgcc
       61 acgaggagga agaacagcac atggaacgag atcaggccat caagttcgtc cacgagctgc
      121 tgcgcgtgat ggtcagcaag aaggcctccg acctgttcat caccgccggc ttcccgcccg
      181 ccttcaaggt ggacggcaag atgacgccgg tgtcgaacca gagcctgacg cccacgcaca
      241 ccatggagat cgcgcgcgcg atcatgaacg acaagcaggc ccaggagttc gaggccacca
      301 aggagtgcaa cttcgccatc agcccgccgg gcatcggccg cttccgggtc aacgccttcg
      361 tgcagcaggg ccgcgtgggc gtggtgctgc gtaccatcac caccaccatc ccgcggctcg
      421 aggacctcgg cctgccggac gtgctcaagg acgtcgccat gaccaagcgc ggcctggtga
      481 tcgtggtcgg cgccaccggc tcgggtaagt ccaccagcct ggcggcgatg ctgggctacc
      541 gaaacgagaa cagccacggc cacatcatca ccatcgagga cccggtcgag tacgtgcacg
      601 accacaagaa ctgcctgatc acgcagcgcg aggtgggggt ggacaccgag tcgtggcatt
      661 ccgcgctgaa gaacacgctg cggcaggcgc ccgacgtgat cctgatcggc gagatccgcg
      721 atcgcgacac catggactat gccgtggcct tcgccgaaac cggccacctg tgcctggcca
      781 ccctgcacgc caacagctcc aaccaggcgc tcgaccggat catcaacttc ttccccgagg
      841 agcggcgtct gcagctgttg atggacctgt cgctcaactt gaagggcatg atctcccagc
      901 gcctgattcc gcggcgcgac ggccccgggc gcgtggccgc gatcgagatc atgctcaact
      961 cgccgctgat ctccgacctg atcttcaagg gcgcagtgca cgagatcaag gagatcatga
     1021 agaagtcccg cgagctgggc atgcagacct tcgaccaggc gctgttcgac ctgtacgagg
     1081 gcgggctgat cagctacgag gacgcgatgc gcaacgccga ctcgatgaac gagctgcggc
     1141 tggcgatcaa actgcacggg cacgaggcca aggagaagga catgctcacc ggcctcgacc
     1201 acctcaatat cgtgtaggta cgaggcgcca ggcacgaggc ctcatgcctc gcgcctcttc
     1261 tcccgcatcg ccccggccac cagctcgaac acatacagcc ggcattccag ggcgccgttg
     1321 tacagcggga tgcggcgcgc gggcttcaac ccgatcagct tcggcagccg caagtcggcg
     1381 gtgaagatgc atgcgcgcca gcccgcaaag cgctgcttga gcgcgtcgcc cagcctgggg
     1441 tagagatcgg cgagcgcctg ctgctcgccc aagcgcacgc cgtaaggcgg attcatcacc
     1501 agcacgccgg ccgccgcggg cgcggagatc tccagggcgt tggcctgctt cagccggacc
     1561 acgccctcca ggcccgcgcg tgacaggttc tcgcgcgcga gcttcaaggc ggctccgtag
     1621 agatcgctgc cgaagatcga cagcggctgc ggcgggcact cggcggcctg cgcctgcgcc
     1681 cgcagcgccg cccaccgcgc cgcgtcgaag ttgtgcagat tctcgaaggc gaagcggcgg
     1741 cggccgccgg gcggccgccg cagcgcggct tcagcggcct cgaccagaaa ggtgccgccg
     1801 ccgcacatcg ggtcgaccag cgggatgccc ggcgcccagc cggcgagatg caggatgccg
     1861 gctgcgaggt tctcgcgcag gggcgcttca gccgccgcca cgcgcaggcc gcgcttgaac
     1921 agcggctcgc ccgaggtgtc gagatacagg ctcaaggtgg ccgcgtcgaa gtaggcgtgg
     1981 atgcgcacgt cgggattggc ggtgtccacg ctgggacgcc ggccgcagtc cgcccggaag
     2041 cggtcgcaga ccgcgtcctt gatgcgtagc gtcacgaagt ccaggctctt gagcgggctg
     2101 ccggtggccg acacgttcac gcggatggtg cgctccggcg cgaaatgctc gcgccagggg
     2161 agcgcggcga cgacccggta gacgtcgtcc tcgtcgcgat acggcgcgtg cgcgagccgc
     2221 cacagcacgc ggctggcgat gcggctgtgc aggttcaccg catagcacag ctcgaattcg
     2281 ccggcgaagc cgacaccgcc gtcgcgcggg tccagctcga gcgcaccgag cgcctcgagc
     2341 tcggcgcgca gcacctcctc gagaccgcgc gggcagggcg agaagaagtg ctggcggccg
     2401 acatcggggc cggatcgcgt gcggctgcgc gggcgttcgg acatgcgcga ttatcgcgca
     2461 ccgcatgccg tcacaggcgc agatagacct cgatctcgag cttgagccgg tggttgcggt
     2521 acttctccag cctggcgatg cgcgcgcgca gcgggttgcc gcggtcgagc tggcgcgcca
     2581 ccgaggcgtt gtccgcccgc ggcagatagc ccagcacgcg gccgttccac tcgacgcgca
     2641 ccgcgttggc atcgtgcgga ttgtcgatct cgcgcaccag cgtaacctcg tcgcccggct
     2701 tcatctgcgc ccacaccgcc ttggcgtcgt ggtggcgcag acccgcggcg agcgtgctgt
     2761 gcaccatgat ctcgctggcg acctccgcgg cgggcgccgc cagcgtggcg agcaccagag
     2821 cggctgcgag aagggcgcgc gccatccagg gaagcttccc tagatcagaa cggcttgacc
     2881 accaccagca gcaccgccgc cagcaggatc agcaccggca cctcgttgaa ccagcggtac
     2941 cagacatggc tgcgccggtt gcgcccgtgc cggaagtcgc ccagcagctt gccgcaccac
     3001 aggtgatagg cgatcagcag cgcgaccagg gcgagtttgg cgtacatcca gccgccggcg
     3061 tcgcggaacc actgcagcca gagccacact ccgaacacga tggtcagcac cgcgcccggt
     3121 gtcatgatgc cgtagtacag cttgcgctcc atcacccgga agcgctcgat gccctgcgca
     3181 tcctgcgcca tggcgtgata cacgaacagg cgcggcaggt agaacaggcc ggcgaaccag
     3241 gtgaccatga acacgatgtg caacgctttg acccacagca tcgcgcaagc ttactgcagc
     3301 agccggcgcc gcgtcaggac cagggccgcg gagaagccgg cggcggcgta gccgaccagc
     3361 acgccgaggt gcagcagcgc ccgctgcggg atctcgccgt tcaccagcgg gcgcaccagg
     3421 tcgatggcat gggtaagcgg cagcacggcc gagacctgtt gcagcagcgg cgggagctgg
     3481 gccaccggga agaacacgcc gcacagcagg accatgggcg tgatcaccag cgtgaagtag
     3541 tacaggaaga aatcgtagct cggcgccagc gcggtcatgg tcaggcccaa cgtgccgaag
     3601 gtcaggccga tgagcagcgt gagcggcacg atccacagcg tgagcgggga atgcgacagc
     3661 gcgagcgccc agatgatgat caggatggcg atgccggaca ggaagctctt ggaggcggcc
     3721 cacagggtct ccgccagcac gatgtcgtcc aggttcaacg gggcgttcat gatcgcgtcc
     3781 caggtgcgct gcacgtgcat gcgcgagaag ccggagtaca gcgcctcgaa ggtcgcgctg
     3841 ttcatggtgc tgtagcacac cgtcccggcg gcgaggaagg tgatgtaggg gatgccgtcc
     3901 acctgcggta gcaggccgcc cagtccgtac ccgagcccga gcatgtagag catggggtcg
     3961 gccaggttgc ccaggatcga cgtgacggcg agcttgcgcc acaccaggaa gttgcgccgc
     4021 catacctgaa cgaagcgcgc ggagatgcgc ggcggagcga agtgcttggc aatcatctgc
     4081 ctccgcctca atcgcgcagg tcgcggccgg tgagcttgag gaacacgtct tcgagattgg
     4141 cccgccggtg caggtagcgc acgtcgcgct gtccctccag cgtgcgcagc accggctcgc
     4201 cgtccccggt gtagcagaac accgtctcgc cggcgcgctc ccagcgccgg cacaggcgct
     4261 gcgcatgggc ctgcgcccag gccggggcgc cctcgccgta cacctccacc acctgcggct
     4321 cgatgtggtg cgcgatcagc tcctgcggcg tgccggtgat gatcaggcgc ccgtggtcca
     4381 tgacgctcag ccggtcgcac aggcgctcgg cttcgtccat gaagtgcgtg gtgagcagga
     4441 tggtcttgcc ctgggcgacc agctgcttca agcgttccca gatcaggtgc cgcgcctgcg
     4501 gatcgaggcc ggtggtgggc tcgtccagga agatcaggtc cggatcgttc accagagcgc
     4561 gcgccagcga cagccgccgc ttcatgccgc cggacagtgc ctggatgcgc gcctgtgcgc
     4621 gcccctcgag cccggcgaac tccagcaggc gcgggatgcg cgccgcgatc gcgtcgtcgg
     4681 gcaggccgaa gtagcgcccg tacaccagca ggttctcgag cacggtgaaa tccgggtcga
     4741 gattgtccag ctgcggcacc acgcccagcc gcaggcgcgc ctcgcgcgcg cgccgcggaa
     4801 tcggctcgcc cagcagtctg atgcggccgg catccggctc ggtgaggccg agcgcgaggc
     4861 gcagcgtcgt ggtcttgccc gcaccgttgg gcccgagcag cccgtagcac tcccccgcgc
     4921 gcaggtccag atcgacgccg cagatcacct cctgcccgcc gtaggacttg cgcacgccgg
     4981 cgaggctcag catcagcagg cgcagctgtg gttgactacg cgtgcaagct cgaccagctt
     5041 gccgtggaag aagtggcccg cgccgggcat caccaccacc ggcaggcttt gcggccgtgc
     5101 ccagtcgagc acctcgcgca gcggcaccac gtcgtcctcg tcgccatgga tcaccagggt
     5161 cccggccggc acctgctgca gcgcgaagcg gttgacggcc ggagccacca gcaccagccg
     5221 cgccggtgcg agcgtgcgcg ccacttgcgc ctgcacgaaa gcgccgaagg aaaagccggc
     5281 cagcgccagg ggcaggtcgc cgaaccggcc ctgcatggcg cgcgccacgt gcagccagtc
     5341 ctgcgtctcg ccgacgcctt cgtcgaacgt cccgtcgctg gcgccgaccc cgcggaagtt
     5401 catgcgcacc gcgacgtgac cctgcgcgtg gaagcagcgc gccagagtct gcaccacctt
     5461 gttgtcgagc gtgccgccgt agagcgggtg cggatgggcg atgagcgcca gccccctgcg
     5521 cgcctcgccg gggtcgctga ccacgatctc gaggtttccg gcgggccccg gcagcagcgc
     5581 tcgctcgtcg cgcgccggcc tcaaaggcgc aaccgctcga cgatccggcc gtgccgcaga
     5641 tgctcctcga tgatctcgtc cacgtcggcc cggtccacgt aggtgtacca gacctcctcc
     5701 gggtagacca cgatcaccgg tccttcctcg cagcggtcca ggcagccggc ctggttgacg
     5761 cgtaccttgc ccggcccgga caggcccagc tccttgacgc gctgcttggc gtagtcgcgc
     5821 atctgctgcg aaccgtggtt gttgcagcag gcctctcccg ccgcgcgctg attgcagcag
     5881 aagaacacgt ggtggcggta gtagctcatg cggcgattat aagatcaacc gcaaaggcgc
     5941 agaggaacgc agaggaaagc gagagacggc tttccggctg ccctgagatc gcacgcgcac
     6001 cgagccgcat gcgcagccgg cgctgggaac gcattccctt gcgttccttt gcgcctccgc
     6061 gcctttgcgg ctggagaggg gtagaagcta ggccaactaa gcagcggcct tggggtcgct
     6121 gtgcacgatg cgctgggcgt tggccgcgtc cagatgctcc aggcggtacc cgtagcggta
     6181 cacggcggcc agccgccagc cgaactcggg agtgaggccg agcttgttgc ggatgcggct
     6241 gacgtgcgta tccagcgtgc gcgaggtgac caccgcattg ggtccccata ccgtctcgcg
     6301 gatgtgaccg cgcgacagca gccggcccac gttgcgcagg aacagcacgg acagatcgaa
     6361 gtccttcgcc gtgaggtcca cctgcttgcc gttgcggtgc acggtgcgcg tctggcaatc
     6421 caccttgaac acgtccagct cgatcacctc ggcctgctcc ggaccgtgcc ggccgcggcg
     6481 ggacagcgct tccaggcgcg cgagcagctc caagcgccgc accggcttga tcatgtagtc
     6541 gtcggcgcct tcgcgcagcg cggtgaccac gtcgtcctcg cggttgcgcc cggtcacgaa
     6601 cagcaccggc accgaggagt gcagccgctg gcgcacgcgc ttgagcacct cgaccccgct
     6661 caggtcgggc acgttccagt cgagcagcag cgcgtcgaaa ctctcgcgct ccagcgcgcg
     6721 cagcagattg gcaccctgct cgaaggcgtg cgcattgtgt ccggcgagct gcagccaatg
     6781 gctcagcagc tcggtctgag agatatcgtc ttccagaatt gcgattcgca tgggctggtt
     6841 gggggcgagt ccagggaagt tgtctgtcga actctggagg accgggctag tcgaagcgca
     6901 cttgcagccg cttcagatag cgctccattg tggtgatgtg cacggcgatc agctcggtgt
     6961 cggcggcccg agccgccagc tcgaacttgg cgccgagaat ggacagctcg ggaaagccga
     7021 acccttcgcc ggctcccttc aaatcgtggc cgagcttgcg gatggtctcg aagtcggcgc
     7081 cggacaatgc ctggcgcagg cgcgcgagct cgtcctcctt cctgcgcagg aagcgcgtca
     7141 ccagctgctc gaggtccttc tcgagccaga ccacgcgcgg ctgcaggctg tcgcgcgtga
     7201 tttcggtcgg gttggtgcgc cgggggcccg tcacaggaca cctccgggac gcgccaggac
     7261 gctttgcacg tgcgcccgca gcacgtcgct gcggatcggc ttggcgagca acgcatcgca
     7321 gccgctcgac aaggcgcgct cggcctccgg cccttcgccg tgaccggtga gcgcatagat
     7381 cggcttggtg tagccgcgca ggcgcagctc cgcgacggcc tcgaatccgg tcatcaccgg
     7441 catgtccatg tccatcagga ccagggccgg ctgccggctg agcgcgagat cgagcgccgt
     7501 gcgcccgttc tcggcctgga gcacgttcac gcccatccga cgcagctgat ggaccacgag
     7561 ctcgcgtatg tcctcgttgt cctcggccac caacacggcc agctctgcct gggtcatgtg
     7621 ctctgatgtc tcggtcgggt ctaagtgccg gtccgggccc cgcacagggg cgccgagccg
     7681 gcgtattgcg gaggaacttt tgcaagacgc gtgccacggg aatccggggg atccggcgtt
     7741 ccggggctcg cccgcccgcg gcggaaggcg gcgcgatcgg tggccgggcc ggggcggatc
     7801 aggcgggcag gatcaagcca gcggaaacag ctccttagac aatactttga cgatcgcgca
     7861 cctcacacgg cggtgcccgc gacgcacatc aggagggtct gtcggctcgc ggagcggtcc
     7921 ccggtctgct cggacaggcg cgcgagccgg cgcacatggc cgttcaccgt atgccgtgct
     7981 gcgaactcgg ggaaggccct ggcgaccact tccagcggtg cgttgatcag cagcccgtac
     8041 tgctctgcgc cctcgctgcc gcccggcgcg aagtagaaag cgctgatgag catcccgcgc
     8101 acggcgcctg cagcgggcag ggagatcagg accccgcccc ggtgcggatc gaccagctcc
     8161 ccata
//
LOCUS       DASPGE010000236         3146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_998798, whole genome shotgun sequence.
ACCESSION   DASPGE010000236 DASPGE010000000
VERSION     DASPGE010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998798"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VD791_07585"
     CDS             <1..357
                     /locus_tag="VD791_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXV07874.1"
                     /translation="GFNQVPPLNNRELFRRDRHTCAYCGGEFGAFKLTRDHITPLSRG
                     GRDTWMNVVTACRHCNGHKRNRLPEECGLELMYAPYVPNKAEYLILTNRRILADQMEF
                     LRQHVAAHSRLVLAPA"
     gene            410..1357
                     /locus_tag="VD791_07590"
     CDS             410..1357
                     /locus_tag="VD791_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HXV07875.1"
                     /translation="MAVTDDFLRAYYLRPEIHPVEESCDAERRLHAALMDAPRRAVPE
                     EEIASVADEDARHNYRMLLAFRDRLLAAGTVEGCYMGLFKGRIDIPPLFIEQLAHVIL
                     RNLLEGCDDPLRLRAAELFFREQKATLQEGHVLLADLETVQMHASGNRYGNLGKLIVE
                     AQGEIGKVDLDVLDRANAALYWERESRHDTVISLTYGRPALDALCRVIESWVLHFHGV
                     AVAVKPLRKIEEARWAWHIGLDAESTEILNALWSGGEVDPGRMRNILALFSMQFEEPA
                     AMRPDIAGRTVYLGLSANAEQVVRMKPQNLLTNLPLHRA"
     gene            1360..1977
                     /locus_tag="VD791_07595"
     CDS             1360..1977
                     /locus_tag="VD791_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HXV07876.1"
                     /translation="MKPDYWDRAKRALARRDPVLAAIMRRHPRVHLVARGEPFQTLAR
                     AIVGQQISVKAAQSVWNRVLEVAPGMTPAEVLALRRPRLRACGLSDRKTEYIADLARH
                     FAEGTVHPHRWPQMDDEAVIEELVAVRGIGRWTAEMFLMFNLLRPDVYPLGDLGLLNA
                     VRLTYFRGRKVPVARIRRLGEQWAPWRSVATWYLWRSLDPVPVEY"
     gene            1982..2950
                     /locus_tag="VD791_07600"
     CDS             1982..2950
                     /locus_tag="VD791_07600"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HXV07877.1"
                     /translation="MKTTFLDFEQPIAELEQRIEELRFVQDDSAVDISEEIQRLTKKS
                     QALTKEIYAKLTPWQVAQVARHPQRPYTLDYVGMLFTGFEELHGDRHFADDASIVGGL
                     ARFDGVPCMVLGHQKGRDTKEKILRNFGMPKPEGYRKALRLMQMAEKFELPVFTFVDT
                     PGAYPGIDAEERGQSEAIGRNLYAMAGLRTPVLTSVIGEGGSGGALAIAVGDVTLMLQ
                     YATYSVISPEGCASILWKSADMAPEAAETLGITAQRLKTLGLVDKVVSEPLGGAHRDP
                     QAAAQSLKKALAEAFRELRSVKAKQLVETRLERLMSYGKFREAAER"
     gene            2919..>3146
                     /locus_tag="VD791_07605"
     CDS             2919..>3146
                     /locus_tag="VD791_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV07878.1"
                     /translation="MASSGKPPSADLAGLELRGRRVAVGLSGGIDSVVLLHLLRALRP
                     REGFRLEALHVHHGLSPRADAWARFCRTLCRR"
BASE COUNT          489 a         1107 c         1094 g          456 t
ORIGIN      
        1 ggcttcaacc aggtgccgcc gctcaacaac cgcgagctgt tccggcgcga ccgccacacc
       61 tgcgcctact gcggcggcga gttcggcgcc ttcaagctga cgcgcgacca catcacgccg
      121 ctgtcgcgcg gcgggcgcga cacgtggatg aacgtggtga ccgcttgccg gcactgcaac
      181 gggcacaagc gcaaccgctt gcccgaggaa tgcggcctgg agctgatgta tgcgccctac
      241 gtgccgaaca aggcggagta cctgatcctc accaaccgcc gcatcctcgc cgaccagatg
      301 gagttcctgc ggcagcacgt ggcggcgcac agccgcctgg tcctggcccc tgcctgagtt
      361 ctggcgtaac agcggctacc gcctgctgca acgggacgcg gccgggctgc tggccgtcac
      421 cgacgatttc ctgcgcgcct attacctgcg cccggagatc cacccggtcg aggaatcctg
      481 cgacgccgaa cggcggctgc atgccgccct gatggacgcg ccgcggcgcg cggtgcccga
      541 ggaggaaatc gcctccgtcg ccgacgagga cgcgcgccac aactaccgca tgctgctcgc
      601 gttccgcgac cggctgctcg ccgcgggcac ggtcgagggc tgctacatgg gcctgttcaa
      661 gggcaggatc gacatcccgc ccctgttcat cgagcagctg gcgcacgtga tcctcaggaa
      721 cctgctggag ggctgcgacg acccgctgcg gctgcgcgcc gcggagctgt tctttcgcga
      781 gcagaaggcg accctgcagg agggccacgt gctgctggcc gacctggaga ccgtgcaaat
      841 gcacgcctcg ggcaaccgct acggcaacct cggcaaactg atcgtcgagg cgcagggcga
      901 gatcggcaag gtggacctgg acgtgctcga ccgcgccaac gccgcgctct actgggagcg
      961 cgaatcgcgt cacgataccg tgatcagcct gacctacggc cgtccggcgc tcgacgcgct
     1021 gtgccgcgtg atcgagtcct gggtgctgca cttccacggc gtcgcggtgg cggtcaagcc
     1081 cctgcgcaag atcgaggagg cgcgctgggc ctggcacatc ggcctggacg ccgagtccac
     1141 ggaaatcctc aacgcgttgt ggtcgggcgg cgaggtggac ccgggccgca tgcgcaacat
     1201 cctcgcgctg ttcagcatgc agttcgagga gcccgcggcg atgcggccgg acatcgccgg
     1261 ccgcaccgtt tacctgggcc tgagcgccaa cgccgagcag gtggtgcgca tgaagccgca
     1321 gaacctgctc accaacctgc cgctgcatcg ggcctgacgg tgaagcccga ctactgggac
     1381 cgcgccaagc gcgcgctcgc gcgccgcgat cccgtgctcg ccgccatcat gcggcgccac
     1441 ccgcgcgtgc acctggtggc gcgcggcgag ccgttccaga cgctggcgcg cgccatcgtc
     1501 ggccagcaga tttcggtgaa ggcggcgcag tcggtgtgga accgggtgct cgaggtggcg
     1561 ccgggcatga cgcccgccga ggtgctcgcg ctgcggcggc cgcgcttgcg ggcctgcggc
     1621 ctgtcggacc gcaagacgga gtacatcgcg gacctcgcgc gccacttcgc cgaggggacg
     1681 gtgcatccgc atcgctggcc gcagatggac gacgaggccg tcatcgaaga attggtcgcc
     1741 gtgcgcggca tcggccgctg gacagcggag atgttcctga tgttcaacct gctgcgcccg
     1801 gacgtctacc cgctgggcga cctgggactg ctgaacgccg tgcgcctgac ctacttccgc
     1861 gggcgcaagg ttccggtcgc gcgcatccgc cgcctgggcg agcagtgggc cccctggcgc
     1921 tcggtggcca cctggtacct gtggcggtcg ctcgacccgg tgccagtaga atactgaccc
     1981 cgtgaagacc accttcctcg acttcgagca gccgatcgcc gagctcgagc agcgcatcga
     2041 ggagctgcgc ttcgtgcagg acgattcggc ggtcgacatc tccgaggaga tccagcgcct
     2101 caccaagaag agccaggcgc tgaccaagga gatctacgcc aagctcacgc cctggcaggt
     2161 cgcgcaggtc gcccggcacc cgcagcggcc ctacacgctc gactacgtgg gcatgctgtt
     2221 caccggcttc gaggagctgc acggcgaccg ccacttcgcc gacgacgcct ccatcgtcgg
     2281 cggcctggcg cgcttcgacg gcgtgccctg catggtgctc ggccaccaga agggcaggga
     2341 caccaaggaa aagatcctgc gcaacttcgg catgcccaag cccgagggct accgcaaggc
     2401 gctgcgactg atgcagatgg cggagaagtt cgagctgccc gtgttcactt tcgtcgacac
     2461 gccgggcgcc tatcccggca tcgacgccga ggagcgcggg cagtcggagg ccatcggccg
     2521 caacctgtac gccatggcgg ggctgcgcac gcccgtgctc accagcgtga tcggcgaagg
     2581 cggctcgggc ggcgcgctgg ccatcgccgt gggcgacgtg acgctgatgc tccagtacgc
     2641 cacgtactcc gtcatctcgc cggaaggctg cgcctcgatc ctgtggaaga gcgccgacat
     2701 ggcgccggaa gcggccgaga cgctcggcat caccgcgcag cgcctgaaga cgctggggct
     2761 ggtcgacaag gtggtcagcg agccgctcgg cggcgcgcac cgcgatccgc aggcggccgc
     2821 gcagtcgctg aagaaggcgc tcgccgaggc gttccgcgag ctgcgcagcg tcaaggcgaa
     2881 gcagctcgtg gaaacgcgcc tcgagcgcct catgagctat ggcaagttca gggaagccgc
     2941 cgagcgctga cctcgccggg ctggagctgc gcggcaggcg cgtcgccgtc gggctctcgg
     3001 gcggcatcga ttccgtcgtc ctgctgcacc tgctgcgcgc gctgcggccg cgcgagggct
     3061 ttcgcctcga agcgctgcac gtgcaccacg ggctgagccc gcgggccgat gcctgggcgc
     3121 gcttttgccg gacgctgtgc cggcgt
//
LOCUS       DASPGE010000237         7365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_179620, whole genome shotgun sequence.
ACCESSION   DASPGE010000237 DASPGE010000000
VERSION     DASPGE010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7365
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179620"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VD791_07610"
     CDS             <1..610
                     /locus_tag="VD791_07610"
                     /inference="COORDINATES: protein motif:HMM:NF021645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HXV07879.1"
                     /translation="MQTPGGRELNAIVDPYSYREAIRQPKLLLLGTNDRYWTLDALNL
                     YWDALPGAKHVLYVPNAGHALHEAHWTDTLACFFEQVAAREALPELQWRHTRTGASLE
                     LSVRAAPAPRKATLWRTTSPTRDFREAQWEALDVPAGAAQVRAEVALSPGEYTAAFIA
                     LGFTLNGRDCSFSTQVRIEPPTQSPARDMRSPDAGRRPESGD"
     gene            complement(628..957)
                     /locus_tag="VD791_07615"
     CDS             complement(628..957)
                     /locus_tag="VD791_07615"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV07880.1"
                     /translation="MAIPTRLTPEDVAQAPLTPGRLSRLAFDTGEIELRYYAPQGEDK
                     QVPHDRDELYFVVRGSGAFLRGGERVPCAAGDVLFAAAGEPHRFADFSADFAVWVLFY
                     GGIKKGG"
     gene            complement(982..1359)
                     /locus_tag="VD791_07620"
     CDS             complement(982..1359)
                     /locus_tag="VD791_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV07881.1"
                     /translation="MEHAFRFSVSHARDAQWDAGLRAYFEYRDLGIKEATAGKIVAHV
                     IRAIPGVQAMPQRHHHEVEFQMVYVLKGWIRFDYEGSGEVTLTAGTCVHQPPGIRHTE
                     LGHSDDLELIEIVLPAEFETFND"
     gene            complement(1455..2189)
                     /locus_tag="VD791_07625"
     CDS             complement(1455..2189)
                     /locus_tag="VD791_07625"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HXV07882.1"
                     /translation="MATQLLIYERAVPVSSQRHAKWSVEVGANYGFSRNVNSVPLMAV
                     EFPVAAAEYPIVFAGAGDGVMPAVILGVRGRENLFLSQENSWQAKYIPAFVRRYPFVF
                     SGSKDNKTFTLCIDEAFAGFNQEGRGQALFDKQGKASEYVQGVLKFLQEYQAQFMRTQ
                     AFCRKVKELDLLEPMQAQVTQGSGERTALTGFQAVNRERLKALAGTKLAQLAKTDELE
                     LIYLHLQSLRNFSGFGERLATHAAAA"
     gene            complement(2298..3089)
                     /locus_tag="VD791_07630"
     CDS             complement(2298..3089)
                     /locus_tag="VD791_07630"
                     /inference="COORDINATES: protein motif:HMM:NF019585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HXV07883.1"
                     /translation="MTAAAPSTLRLYLDDSHCFETDACVVAVRGDALAFDRTCCYPGG
                     GGQPADQGRARLESGRALEIVSVQADADDVLWHVVKEAPRPDLIGQGHLIGQEDLIGQ
                     GDLTGQAARLTVDRERRLALARYHTVLHVLNTIALRDYGGWITGCQIAPDYARIDFKL
                     DALSAELCAELERKVNAVLAADHPVTHRYLPEEEFRRRDDLLRTLEAKPPVVDGRVRV
                     VEIRGFDAQACGGTHVRSTAELGRLTIFRTENKGRINKRLYVRLG"
     gene            3221..4582
                     /locus_tag="VD791_07635"
     CDS             3221..4582
                     /locus_tag="VD791_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXV07884.1"
                     /translation="MEQALPKVCVIGAGSSGLPVIKALKERGVPVTCFEKTPNIGGLW
                     CIGNRAFGASAAYDSLHINTDTRMMEYRDYPMPDDIPAYPGHAEIHRYFEAYARHFDL
                     HPHIRLETPVTHARRRADGVWEITAGGERLEFDALVVANGHHWEPRWPEPYPGHFAGQ
                     QIHSHDYRNPADPVVMRGKRVLVVGIGNSAVDIASELGHRSTGARCILSLRRGAWIMP
                     KWVLGFPLTRPFVSVPHWVPWRLGAVVLDLTARLVFGTPMNYGLPRPDHKWLRAHPTI
                     SQDLYSRLGHGDIAVKPAIEAFDGQRVRFTDGSAEEIDVVVWCTGYKVSFPFFDAEFL
                     EAKGNYLPLWQRMLLPGVDNLFFVGLYQPLGSVMQPAELQAKVIADYLCGELAFPDAD
                     TMRREMVAMQDANDRRFVKSARHTMEVDFVPFLHALRKLRARGRRLAARRGGRHPVAA
                     RAH"
     gene            complement(4711..5835)
                     /locus_tag="VD791_07640"
     CDS             complement(4711..5835)
                     /locus_tag="VD791_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXV07885.1"
                     /translation="MRITDIQVLELAGPRLPAAMRPAWAPGSEWRRWGGTLVKVFTDE
                     GIVGLGSPGYKVSPLIESWIKPQLVGQDPFALERHARIFRNAGAGWGVEIALWDIIGK
                     ACGQPVYKLWGGFRDRVPAYASCIEVRSPPQRAEDIAGVKAGGWCAAKLRIHDWTVKA
                     DIAQVEAVRRAVGEDFTLLVDANQAQLPGTLQPDAGPVWSYERALHTARELERLAVYW
                     LEEPLGRYDFDNLSRLAAAVDILIAGGENNLGLHEYRWLIDRGCYDVLQPEAMVGETM
                     SQIRKACAYAEMHHKLCAPHHGGGGLGFVAHLHLCAALPNCPFVEVFHDPPGFTGDLF
                     QWYLAEPIRLDPSDGCMALPQKPGLGVELDEEKIRRFAVG"
     gene            complement(5832..6125)
                     /locus_tag="VD791_07645"
     CDS             complement(5832..6125)
                     /locus_tag="VD791_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07886.1"
                     /translation="MQLRSTHLLVIAGIALLLLAVAGVVYMDQREQARAQRAKGAERP
                     RSATPGPSGRERCMQKCAAAKKGYIYRAEQRVEEAGRSRIVPEACRCLGREVK"
     gene            complement(6147..6347)
                     /locus_tag="VD791_07650"
     CDS             complement(6147..6347)
                     /locus_tag="VD791_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07887.1"
                     /translation="MPIPRALKIGAGVAASFIAFAVAIMLLSDTGVVTFQMALLMLVA
                     LFGLYLGFGILIAVYRVVSKLD"
     gene            complement(6420..6803)
                     /locus_tag="VD791_07655"
     CDS             complement(6420..6803)
                     /locus_tag="VD791_07655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HXV07888.1"
                     /translation="MLLVRVRVAASGIHGLGVFAVAPIPKGTVVWRFTPGFDLDLDPG
                     VLGAQPAHFREVLLHYGYIDPRLNRFILCCDDARFINHSETPNLLVDAAGDRYGVDVA
                     ARDIAAGEEMTMDYALVEGSRPAPP"
     gene            complement(6830..7198)
                     /locus_tag="VD791_07660"
     CDS             complement(6830..7198)
                     /locus_tag="VD791_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV07889.1"
                     /translation="MPALTDLRCVLAVKDLAVSVAFYRERLGFNLDFEVPGWAFLSRD
                     RFRVMLGECADAMAAHETGDHSYFAYVTVDGIDELYREFVEKGVLRVQDLSNKPWGMR
                     EFGVRTPDGHRIMFGQGIPT"
BASE COUNT         1172 a         2587 c         2469 g         1137 t
ORIGIN      
        1 catgcagacg ccgggcgggc gtgagttgaa cgcgattgtc gatccgtaca gttatcgcga
       61 ggccattcgc cagcccaagc tgctcttgct gggcaccaac gaccgctact ggacgctgga
      121 cgccctgaac ctgtattggg acgccctgcc cggcgcgaag cacgtcctgt atgtgcccaa
      181 cgcggggcat gctctgcacg aggcgcactg gaccgacacg ctggcctgct tcttcgagca
      241 ggtggcggcg cgggaggcct tgcccgagct gcaatggcgg cacacgcgca cgggcgcgag
      301 cctcgagctg tcggtgcgcg ccgcgcccgc gccccgcaag gcgacgctgt ggcgcacgac
      361 ctcgcccacc cgcgacttcc gcgaggcgca atgggaggcg ctcgatgtgc cggccggtgc
      421 ggcgcaggtg cgggccgagg tggccctctc tccgggcgag tacaccgccg cattcatcgc
      481 gttgggtttc acgctgaacg ggcgcgactg cagcttctcg acgcaggtgc gcatcgagcc
      541 gccgacgcaa tctccggccc gtgatatgcg tagcccggat gcaggccgaa ggccggaatc
      601 cggggattga tctggcggct ccgcgcctca gccgcccttc ttgatgcccc cgtagaacag
      661 cacccacacg gcgaagtcgg cgctgaagtc ggcgaaccgg tgcggctcgc cggcggcggc
      721 gaacagcacg tcgccggccg cgcacggcac gcgctcgccc ccgcgcagaa acgcgccgga
      781 gccgcgcacc acgaagtaca gctcgtcgcg gtcgtgcggc acctgcttgt cctcgccctg
      841 cggcgcgtag taacgcagct cgatctcgcc ggtgtcgaag gcgagccgcg acaggcgccc
      901 cggcgtgagc ggtgcttgcg cgacgtcctc gggtgtgaga cgggttggga tcgccatggg
      961 cgcgcctgcg gtggcggccc gctagtcgtt gaacgtctcg aactccgccg gcagcacgat
     1021 ctcgatcagc tccaggtcgt cggagtggcc cagctcggtg tggcggatgc ccggcggttg
     1081 atgcacacag gtaccggcgg tcagggtcac ttcgccggag ccctcgtagt cgaagcggat
     1141 ccagcccttg agcacgtaca ccatctggaa ctcgacctcg tgatgatgcc gctggggcat
     1201 cgcctgcacg cccggaatgg cgcggatcac gtgggcgacg atcttgccgg cggtggcttc
     1261 cttgatgccc aggtcgcggt actcgaagta ggcgcgcaac cccgcgtccc actgcgcgtc
     1321 cctggcgtgg ctgacggaaa acctgaaagc gtgctccatc gcgcgtctcc tgtggggcgt
     1381 cgatgccgcg attataggct gcgaggcgcg cgcaggcggc agccgccccg gcacaggggc
     1441 tgctggaggg ttcgctaagc cgcggcggcg tgggtggcca ggcgctcgcc gaagccggag
     1501 aagttgcgca gcgactgcag gtgcaggtag atcagctcca gctcgtcggt cttcgccagc
     1561 tgcgccagct tggtcccggc gagcgccttg agccgctcgc ggttcaccgc ctgaaacccg
     1621 gtgagcgcgg tgcgttcgcc ggagccctgc gtgacctgtg cctgcatcgg ctcgagcaga
     1681 tcgagctcct tcaccttgcg gcagaacgcc tgcgtgcgca tgaactgcgc ctggtattcc
     1741 tgcaggaact tgaggacgcc ctgcacgtac tcgctggcct tgccctgctt gtcgaacagc
     1801 gcctggccgc gtccctcctg gttgaagccg gcgaaggcct cgtcgatgca cagcgtgaag
     1861 gtcttgttgt ccttgctgcc ggagaacacg aacgggtagc ggcgcacgaa ggccgggatg
     1921 tacttggcct gccagctgtt ctcctgcgac agaaacaggt tctcgcggcc gcgcacgccc
     1981 aggatcaccg ccggcatgac gccatcgccg gcgccggcga acacgatcgg gtactcggcg
     2041 gcggcgaccg gaaactccac cgccatcagc ggcaccgagt tgacgttgcg gctgaagccg
     2101 tagttggcgc cgacctccac cgaccacttg gcgtggcgct ggctggagac ggggacggca
     2161 cgttcgtaga tcaggagctg ggtggccatg ggcaggctgg gttgggtgga cgatggcgcc
     2221 ggcaggagca ggtttcgggc caacgccggg ggcaacggcc ccggccgcgg gtcgagcggg
     2281 cgtcgaaagg cggccggtca gcccagcctg acgtacaggc gcttgttgat cctgcccttg
     2341 ttctcggtgc ggaagatcgt gagccgtccc agctccgcgg tgctgcgcac gtgcgtgccg
     2401 ccgcaggcct gcgcatcgaa cccccggatc tcgaccacgc gcacgcgccc gtccaccacc
     2461 ggcggcttcg cctccagggt gcgcagcaga tcgtcgcgcc ggcggaattc ctcctcgggc
     2521 aggtagcgat gcgtgaccgg atggtctgcg gcgaggaccg cgttcacctt gcgctcgagt
     2581 tccgcgcaca gctcggcgga cagcgcatcg agcttgaagt cgatgcgcgc atagtcgggc
     2641 gcgatctggc agccggtgat ccagccgccg tagtcgcgca gcgcgatggt gttcaacacg
     2701 tgcagcacgg tgtggtagcg cgccagcgcc agccgccgct cgcggtccac cgtcagccgc
     2761 gccgcctgcc cggtcaggtc cccctgcccg atcaggtcct cctgcccgat cagatgcccc
     2821 tgcccgatca aatccgggcg cggcgcttcc ttcacgacgt gccacagcac gtcgtcggca
     2881 tctgcctgaa cggacacgat ctccagcgct cgaccgctct ccagccgcgc ccggccctgg
     2941 tccgcgggct ggccgccgcc gccggggtag cagcaggtcc ggtcgaaggc cagcgcatcg
     3001 ccgcgcaccg ccaccacgca tgcatcggtc tcgaagcaat ggctgtcgtc gagatacagg
     3061 cggagggtgg aaggggctgc ggcggtcacg gcgtcgtcct ggtcgcgttc gcggcattgt
     3121 gtacgcgaaa accccgcccg cggcaagccg accccgcatc gcacccgggc cggcctgcgt
     3181 ttcacctgct agactcgagc cgttcgcgaa tgggggtgag atggagcaag cgctgcccaa
     3241 ggtatgcgtg atcggcgccg gctcgagcgg cctgccggtg atcaaggcgc tcaaggagcg
     3301 cggcgtgccg gtcacctgct tcgagaagac gccgaacatc ggcgggctgt ggtgcatcgg
     3361 caaccgcgca ttcggggcga gcgcggccta cgactcgctg cacatcaaca ccgacacccg
     3421 catgatggag taccgggact acccgatgcc ggacgacatc ccggcctacc ccggccacgc
     3481 cgagatccac cgctacttcg aggcctacgc gcggcacttc gatctgcacc cgcacatccg
     3541 gttggagacc ccggtgacgc atgcgcggcg gcgcgccgac ggcgtgtggg agatcaccgc
     3601 ggggggcgag cgtctcgagt tcgacgcgct ggtggtggcc aacgggcacc actgggagcc
     3661 gcgctggccc gaaccctatc cggggcattt cgccggacag cagatccatt cccacgatta
     3721 ccgcaaccca gccgatcccg tcgtcatgcg cggcaagcgc gtgctggtgg tgggcatcgg
     3781 caacagcgcc gtggacatcg cctccgagct gggtcatcgc tccacgggcg cccgttgcat
     3841 cctgtcgctg cgccggggcg cgtggatcat gcccaagtgg gtgctgggct ttcccctgac
     3901 gcgcccgttc gtcagcgtgc cgcactgggt gccgtggcgc ctgggcgcgg tggtcctgga
     3961 cctgaccgcg cggctggtgt tcggtacgcc gatgaattac ggcctgccca ggccggacca
     4021 caagtggctg cgcgcccacc ccacgatcag ccaggacctc tactcgcgcc tgggtcacgg
     4081 cgacatcgcg gtcaagcccg cgatcgaggc gttcgacgga cagcgcgtgc gcttcaccga
     4141 cggcagcgcg gaggagatcg acgtcgtcgt ctggtgcacc ggctacaagg tcagctttcc
     4201 gttcttcgac gcggagtttc tcgaggccaa gggcaactac ctgccgctgt ggcagcgcat
     4261 gctgctgccc ggcgtcgaca acctgttctt cgttggcctg tatcagccgc tgggatcggt
     4321 gatgcagccc gccgagctgc aggccaaggt gatcgccgac tatctgtgcg gcgagctggc
     4381 gtttccggac gcggacacca tgcgccggga gatggtcgcg atgcaggatg cgaacgatcg
     4441 ccgcttcgtg aagtcggcgc gccacaccat ggaggtggac ttcgtgccgt tcctgcacgc
     4501 gctgcgcaag ctgcgcgcgc gcggcaggcg gctcgcggcc cggcgcgggg gcaggcaccc
     4561 ggtggctgcg cgcgcgcatt gaccgagaac accacaccgc cccggcgcct gcggcgccac
     4621 cccgcctcgt gagaggcggg gacctgtagg ttcccctcct cgtcgaggag gggtggcacg
     4681 cgaagcgtga cggggtggtc cggcggcccc tcaacccaca gcgaagcgcc ggatcttctc
     4741 ctcgtccagc tccacgccga gccccggctt ctgcggcaac gccatgcaac cgtcgcttgg
     4801 gtcgagccgg atcggctcgg ccagatacca ctggaaaagg tcgccggtga agccgggcgg
     4861 gtcgtggaac acctccacga acggacagtt gggaagcgcc gcgcacaagt gcaggtgcgc
     4921 cacgaaaccg agcccgccgc cgccgtgatg cggcgcgcac agcttgtggt gcatctcggc
     4981 gtaggcgcag gccttgcgga tctgcgacat ggtctcgccc accatcgcct cgggctgcag
     5041 tacgtcatag cagccgcggt cgatcagcca tcgatactcg tgcagcccga ggttgttctc
     5101 gcccccggcg atcaggatgt ccaccgccgc ggccaggcgt gacaggttgt cgaagtcgta
     5161 gcgcccgagc ggctcctcca gccagtacac agcgagccgc tccagctcgc gcgcggtgtg
     5221 cagcgcgcgc tcgtaactcc agaccggccc cgcgtccggc tgcagcgtgc ccgggagctg
     5281 cgcctggttg gcgtccacca gcaaggtgaa gtcctcgccc accgcgcgcc gcaccgcctc
     5341 gacctgtgcg atgtcggcct tcacggtcca gtcgtggatg cgcagcttgg ccgcgcacca
     5401 gccgccggcc ttcacgccgg cgatgtcctc ggcacgctgc ggcggcgagc gcacctcgat
     5461 gcacgaggcg tacgccggca cgcggtcgcg gaaaccgccc cacagcttgt acaccggctg
     5521 gccgcaggcc ttgccgatga tgtcccaaag cgcgatctcc acgccccagc ccgcgccggc
     5581 gttacggaag atgcgcgcat gccgttccag cgcgaaaggg tcctggccga cgagctgcgg
     5641 cttgatccac gactcgatca ggggcgagac cttgtagccg ggcgaaccga ggccgacgat
     5701 gccctcgtcg gtgaacactt tcaccagcgt gccgccccag cgccgccact cgctgcccgg
     5761 cgcccaagcc ggccgcatcg ccgccggcag ccgcggcccg gccagctcca gcacctggat
     5821 gtcggtgatc ctcacttcac ctcacgtccc aggcagcgac aggcttcggg cacgatccgc
     5881 gaacgccccg cctcctcgac acgctgctcg gcccggtaga tgtagccctt tttcgcggcc
     5941 gcgcacttct gcatgcagcg ttctctccca gacggcccgg gcgttgcgga tcggggccgt
     6001 tccgccccct tggcgcgctg cgctcgcgcc tgctcccgct ggtccatgta caccaccccc
     6061 gcgaccgcca gcaggagcag cgcgatcccg gcgatgacga gcagatgtgt cgacctcaat
     6121 tgcatgatcg gaagccccga gcgcggtcaa tccagcttgc tgacgacgcg atagacggcg
     6181 atcaagatcc cgaatcccag gtacagcccg aacaacgcca ccagcatcaa cagcgccatc
     6241 tggaacgtca cgactccggt atccgacagc agcatgatcg ccacggcgaa ggcgatgaac
     6301 gacgcggcga cgccggcgcc gatcttcagc gctctaggta tcggcatggc gacggcggcc
     6361 cccgacgacg cggcagttcg acaccgtggc aggcggttcg gcgcacactg gcggccggat
     6421 catggcggtg ccggccggga tccttcgacc agcgcgtagt ccatcgtcat ctcctctccg
     6481 gcggcgatgt cgcgcgcggc gacatccacc ccatagcgat cgcccgcggc atccaccagg
     6541 aggttcggcg tctcgctgtg gttgatgaag cgcgcgtcat cgcagcacag gataaagcga
     6601 ttcaaccgcg gatcgatgta gccataatgc agcaaaacct cgcgaaagtg tgcgggctgc
     6661 gcgccgagca ccccggggtc caggtccaga tcgaacccgg gcgtgaagcg ccagaccacc
     6721 gtccccttcg ggatcggcgc gacggcgaag accccgaggc cgtggatccc gctggcagcg
     6781 acgcggactc gaacgagcaa caccgggctt ctcgctcacg cagttgcgat cacgtcggaa
     6841 tcccctgtcc gaacatgatc cggtgcccgt ccggagttct cacgccgaac tcgcgcattc
     6901 cccacggctt gttggaaagg tcctgcactc gcaggacgcc cttctcgacg aactcgcgat
     6961 acagctcatc gatgccatcc accgtaacgt acgcgaaata cgagtgatcg cccgtttcgt
     7021 gcgccgccat cgcatccgcg cactccccga gcatgacccg gaatcggtcg cgggacagga
     7081 acgcccagcc cggcacttcg aaatccaggt tgaacccgag cctctcgcga tagaaggcga
     7141 ccgagaccgc gagatccttc accgcgagca cgcagcgaag atcggtcagc gcaggcatct
     7201 gcgggctatg cgacgtcact ggatcacctg atcagctagt caggcttgta gtggtactcg
     7261 aactgcttgc gtttggcaag cgagcggccg taggagtcga ggctctgtgc caggactcga
     7321 aatcccaggg gcagcacaaa tagccacagc gtgggtggct caaac
//
LOCUS       DASPGE010000238         3214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1117011, whole genome shotgun sequence.
ACCESSION   DASPGE010000238 DASPGE010000000
VERSION     DASPGE010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117011"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VD791_07665"
     CDS             complement(<1..715)
                     /locus_tag="VD791_07665"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HXV07890.1"
                     /translation="MRAYELKPVQSLDSLTLVERPDPRPGPGQVLIAPRAWSLNYRDL
                     NVAKGAYGGPPPAGRIPLSDGAGEVVEVGPGVTRVKAGDRVAGCFMQGWIAGAPSAEA
                     AATALGGAIDGMLAERVVLSEQGVVKVPEHLSDEEAATLPCAAVTAWNALVREARVKA
                     GDVVVLLGTGGVSLFALQFAKLHGARCIITSSSDDKLALARQLGADDPINYKNRPDWD
                     KAVLELTGGRGADVVVEVGG"
     gene            complement(865..1347)
                     /gene="ssb"
                     /locus_tag="VD791_07670"
     CDS             complement(865..1347)
                     /gene="ssb"
                     /locus_tag="VD791_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HXV07891.1"
                     /translation="MASVNKVILIGNLGRDPEVRYLPSGDAVANFSIATTEKWKDKNG
                     DMQEQTEWHRIAFFGRQAEICGEYLRKGSSVYVEGRLQTRKWTDKDGNERTTTEIRGD
                     RMQMLGGRGGGSAEMREASDNGGAESAPPAKKPAVAAAAAAGARKSTGFDDLDDDIPF
                     "
     gene            complement(1411..2607)
                     /locus_tag="VD791_07675"
     CDS             complement(1411..2607)
                     /locus_tag="VD791_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV07892.1"
                     /translation="MSAAEWRASLGLAGIFGLRLLGMFIILPVFALYAEHLPGGDSHT
                     LVGIALGAYGLTQALLQIPFGWLSDRWGRKPAIYLGLVVFALGSFLAAGAQDIYVVIL
                     GRIVQGAGAVSAVVIALTADLTRDEQRTKAMAIIGVSIGATFGVSMVAGPLLSRIAGV
                     PGIFALTGVLALAALAVVRWVVPDPVAHVRAGNDGDGRTGHPSSFASVLAHPELARLN
                     YGIFVLHAVLMALFVVVPFSLRDAGLPLERHWLVYLPVMAGSVLLMVPPLILSERRGQ
                     QKLAFLASIAVLLAAQALLALPEPARVDIAIALLVFFVGFNLLEASLPSLISKAAPPE
                     ARGTATGVYSSVQFLGTFAGATAGGFVSQHFGAAAVFLLCGLLTMSWLLVAWPMRAPA
                     TVGLRT"
     gene            2657..>3214
                     /locus_tag="VD791_07680"
     CDS             2657..>3214
                     /locus_tag="VD791_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07893.1"
                     /translation="MENAVRYSSSLPCAPGPDCIGARIATCTGAWSSTVAVDFIRIPM
                     DFIRIRGARTHNLKNIDLELPRNRLVVITGLSGSGKSSLAFDTLYAEGQRRYVESLSA
                     YARQFLQLMEKPDVDLIEGLSPAIAIEQKATSHNPRSTVGTVTEIHDYLRLLFARAGE
                     PQCPDHGITLAAQSVAQMVDHAMSLP"
BASE COUNT          515 a         1166 c         1055 g          478 t
ORIGIN      
        1 cgccgccgac ttcgaccacc acgtcggcgc cgcggcctcc ggtcagttcc agcacggcct
       61 tgtcccaatc gggccggttc ttgtagttga tcggatcgtc agcgccgagc tgccgggcga
      121 gcgccagctt gtcgtcgctg ctggaggtga tgatgcagcg tgccccgtgc agcttggcga
      181 actgcaacgc aaacagcgag actccgccgg tgcccagcaa gacgacgaca tcgccggcct
      241 tgacccgcgc ctcgcgcacc agcgcgttcc acgcggtgac agccgcgcaa ggcagcgtcg
      301 ccgcctcctc gtcgctgaga tgctccggca ccttcaccac gccctgctcc gacagcacca
      361 cgcgctccgc gagcatgccg tcgatggcgc cgcccagggc ggtggcggcc gcttcggcgc
      421 tgggcgcccc cgcgatccag ccctgcatga agcagcccgc cacgcggtcg ccggccttga
      481 cccgcgttac ccccgggccc acctcgacca cttcgcccgc gccgtccgag agcgggatgc
      541 gccccgccgg cggcgggccg ccatacgcgc ccttcgccac gttcaggtcg cgatagttga
      601 gcgaccacgc tctgggcgcg atcaacactt gcccgggccc tggcctcggg tcgggacgct
      661 cgaccagcgt caagctgtcg aggctttgca cgggtttgag ttcataggct cgcatgtcgt
      721 gtcctcccgg cgcgcaggtg cgtagcccgg atgcaggccg aaggccggaa tccgggatgc
      781 gagatgcctg tcccttcccg gattccgctc cgctccatcc gggctacgcg gtcttcgcgg
      841 caccaaccga taatcgatag cagctcagaa tggaatatcg tcgtccagat cgtcgaagcc
      901 ggtggatttc ctcgcgccgg ccgcggccgc cgcggccacc gccggcttct tcgccggtgg
      961 cgcgctctcg gcgccgccgt tgtccgacgc ctcgcgcatc tccgcgctgc cgccgccgcg
     1021 gccgccgagc atctgcatgc ggtcgccgcg gatctcggtg gtggtgcgct cgttgccgtc
     1081 cttgtcggtc cacttgcgcg tctgtagccg cccttcgacg tacaccgagc tgcccttgcg
     1141 caggtactcg ccgcagatct cggcctgccg cccgaagaac gcgatgcggt gccattcggt
     1201 ctgctcctgc atgtcgccgt tcttgtcctt ccacttctcg gtggtggcga tgctgaagtt
     1261 cgccacggcg tcgccgctgg gcaggtaacg cacttccggg tcgcggccga ggttgccgat
     1321 caagatcact ttgttcacgg atgccatctg ttcactcctc gatttcaggc ggagcggttc
     1381 acttcatcgc ctgcgcaccc cccgcctggt tcaggtgcgc aggcccacag tggccggcgc
     1441 ccgcatgggc caggccacca gcagccagga catggtcagc agcccgcaca gcaggaacac
     1501 cgccgccgcg ccgaagtgct gcgagacgaa cccgccggcg gtggcaccgg cgaacgtgcc
     1561 gagaaactgc acgctgctgt acacccccgt ggcggtgccg cgcgcctccg gcggcgccgc
     1621 cttcgagatc agcgagggca ggctcgcctc cagcaggttg aagccgacga agaacaccag
     1681 cagcgcgatg gcgatatcca cgcgtgcggg ctcgggcagc gcgagcagcg cctgggctgc
     1741 aagcagcacg gctatcgacg ccagaaaggc aagcttctgc tgcccgcgcc gctcggacag
     1801 gatcaggggc ggcaccatca gcagcaccga cccggccatc accggcagat acaccagcca
     1861 gtggcgctcc agaggcaggc cggcgtcgcg caacgagaag ggcaccacca cgaacagggc
     1921 catgagcacc gcgtgcagaa cgaagatacc gtagttcagg cgcgccagct cggggtgcgc
     1981 gagcacactg gcgaagctcg atgggtgtcc cgtccggcca tcgccgtcat ttcccgcgcg
     2041 cacgtgggcg accggatccg gcaccaccca gcgcaccacg gcgagcgccg cgagcgccag
     2101 cacaccggtg agcgcgaaga tgcccggcac gcccgcgatc cggctcaaca gcggccccgc
     2161 gaccatggac acgccgaagg tggcaccgat gctgacgccg atgatcgcca tcgccttggt
     2221 gcgctgctcg tcgcgcgtga ggtcggcggt gagcgcgatc accaccgcgg agacggcgcc
     2281 cgccccctgc acgatgcggc ccaggatcac gacatagatg tcctgggcgc cggcggcgag
     2341 gaagctgccc agcgcgaaca cgacgagccc gaggtagatg gccggcttgc ggccccagcg
     2401 gtcggacagc cagccgaagg ggatctgcag cagcgcctgg gtcaggccgt aggcgcccag
     2461 cgcgatgccc accagggtgt ggctgtcgcc gccgggcagg tgctcggcgt acagcgcgaa
     2521 caccggcagg atgatgaaca tgcccagcag ccgcaacccg aaaatcccgg ccagcccgag
     2581 gcttgcgcgc cactccgcgg cgctcatgcg gtcgcctgcc gacatgcctg atcgagagga
     2641 aggggggagg ttgggaatgg aaaacgcagt gcgatatagt agcagtttgc cctgcgcgcc
     2701 cggccccgac tgcatcgggg cccgaatcgc gacctgcacg ggcgcgtggt cctcgacggt
     2761 ggccgtggat ttcattcgta tcccgatgga tttcattcgt atcaggggcg cccgcaccca
     2821 caacctcaag aacatcgatc tcgagctgcc ccgcaaccgg ttggtcgtga tcaccggatt
     2881 gtcgggttcc ggcaagtcct cgctcgcgtt cgacacgctg tacgccgagg gacagcggcg
     2941 ctacgtggag tcgctgtcgg cttacgcccg gcagttcctg cagctcatgg agaagccgga
     3001 cgtggacctg atcgagggtc tgtcgccggc catcgcgatc gagcagaagg ccaccagcca
     3061 caacccgcgc tcgacggtgg gcaccgtcac cgagatccac gactacctgc ggctgctgtt
     3121 cgcccgcgcc ggcgagccgc agtgccccga tcacggcatc acgctcgccg cccaaagcgt
     3181 ggcacagatg gtggatcacg cgatgagcct gccg
//
LOCUS       DASPGE010000239        20372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1117411, whole genome shotgun sequence.
ACCESSION   DASPGE010000239 DASPGE010000000
VERSION     DASPGE010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20372
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117411"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..1950
                     /locus_tag="VD791_07685"
     CDS             277..1950
                     /locus_tag="VD791_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HXV07894.1"
                     /translation="METLQAMRDLPRLHEITLVLIRHGFGDLVRRAGVAGLLERAGQV
                     LRWGARAQVAQLTPPQRVRMALSELGPTFVKLGQVLATRGDLFPPDWIAEFETLQANV
                     PPVAFDTLLPEIERALGRSPLELFRELEREPLGAASIAQVHGAKLPDGTPVVVKVMRP
                     GIRPKIEADLRILGHLAAMVEAEMPEARRFQPVQMVAEFRRSMHRELDLALEARAQER
                     FAHNFAGDPNVLIPRIYWEYTRETLNVQQRVVGIPGNDLAAVDAAGLDRALLARRGAD
                     AVLKMILMDGYFHADPHPGNVFYLPGNRLAMIDFGMAGRLTERRRLQIVEMLWAVARR
                     EESGIVEVLLEWVSDAEVDEARLAADVAEMLLDYAHLALKDLRLGTLLQDIVGVIRRH
                     SIVLPADLALLFKALITLEGLGRQLDPEFRLVEHLTPFLTRLMRTRYRPRALLKRGRE
                     RLLEIASLVSSMPEDLRRLVKEARRGRMKMELDLKRLDHFGHQLDRSANRLTVGIITA
                     ALIVGSSIVMTVEGGPTLFGLPLFGFLGFLLAFVAGIGLVVSIWRSRRE"
     gene            2114..2446
                     /locus_tag="VD791_07690"
     CDS             2114..2446
                     /locus_tag="VD791_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXV07895.1"
                     /translation="MPIYEYRCGSCGFEKEYLQKLSDAPITDCPTCGKPAMTKLVSAA
                     GFQLKGTGWYATDFKNGSRKAPGASGKGGAPGKGDAEGSGTGEAKKDTPKADAAPACG
                     AGGCPACQ"
     gene            2455..3087
                     /locus_tag="VD791_07695"
     CDS             2455..3087
                     /locus_tag="VD791_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HXV07896.1"
                     /translation="MKKYFVTGLLIWIPLAITVWLLNLIVTTMDQTLWLLPASLHPSA
                     WLGVHIPGLGLLLSLLVVLLTGVLAANIIGQRLVQIWEAMLARIPVVKSIYNSVKQVS
                     DTLLSSTGEAFRKAVLVQYPREGAWTIAFLTGVPGGDVATHLRGDYVSVYVPTTPNPT
                     SGFFLMLPRAEVIELNMSVDAALKYVISMGVAGPAPPARKAPAPKPVVTP"
     gene            3164..4939
                     /gene="aspS"
                     /locus_tag="VD791_07700"
     CDS             3164..4939
                     /gene="aspS"
                     /locus_tag="VD791_07700"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HXV07897.1"
                     /translation="MRTDYCGLIDKRHLGRNVTLFGWVHRRRDHGGVIFIDLRDREGL
                     VQVVVDPDTPETFATADRARGEYVLRVEGRVRPRPAGTVNPNLVSGEVEVLAKDIEVL
                     NASLTPPFQLDEDGLGENVRLEHRVIDLRRPEMQRNLRLRYRVAMAVRRHLDAEGFLD
                     IETPILTKSTPEGARDFLVPSRMHAGEFYALPQSPQLFKQLLMIAGFDRYYQITRCFR
                     DEDLRADRQPEFTQIDVETSFLGVPEITDLMERMIVAVFKEVMAVDLPTPFPRITYAE
                     AMSRYGSDKPDLRVPLTLTELTDVMKGVEFKVFSAAANSAGGRVAALRVPRGGELTRS
                     EIDAYGEFVAIYGAKGLAYIKVNDAGRGRDGLQSPVVKNLSDAALQAIVQRTGAENGD
                     LIFFGADKAKVVNESLGALRAKLGHERGFVQAGWRPLWVLEFPMFEWNEEEKRWDALH
                     HPFTSPMEGHDNWLDGDPGKALSKAYDMVLNGWEIGGGSVRIHRQEVQAKVFSALNIR
                     PEEARAKFGFLLDALQYGAPPHGGIAFGLDRIVALMAGTQSIRDVIAFPKTQRGQCLL
                     TRAPSPVDERQLRELHIRVRAPEKQ"
     gene            5052..5498
                     /gene="nudB"
                     /locus_tag="VD791_07705"
     CDS             5052..5498
                     /gene="nudB"
                     /locus_tag="VD791_07705"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HXV07898.1"
                     /translation="MATQYKIPRSVLVVVHTSDLQVLLLERADHPGFWQSVTGSQHEG
                     ETLEQTAVREVAEETGLDAARHRLLDWGMRNDYEIYREWRWRYAPGVTHNTEHVFGLT
                     VPQPIDVRIAPREHLAFVWLPWREAADKCFSWSNAQAIRNLPEKVS"
     gene            5525..6280
                     /locus_tag="VD791_07710"
     CDS             5525..6280
                     /locus_tag="VD791_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HXV07899.1"
                     /translation="MPNLHVLTYNIHKGFPQFNRRVSLHELRDRLQSVEPDLVFLQEV
                     VGFHARHAERHAEWPTSPQYEFLADQVWRDFAYGRNAVVDDAHHGNAILSKFPITRWD
                     NEDVSANQLERRGLLHCEIEIPGWDRRLHAINVHLGLLRVWRDRQLLALVHRIERLVP
                     HDAPLIVAGDFNDWTRKASRLLMRSLDVSEVFETYGHGPARSFPAMLPVLHLDRIYVR
                     GFRIKLAHVHRGLHWSRISDHAALSALLMREQA"
     gene            6413..8590
                     /locus_tag="VD791_07715"
     CDS             6413..8590
                     /locus_tag="VD791_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HXV07900.1"
                     /translation="MLWVLGSLCQIYRIPFDAKLVLQRFAPPYTLATLCEAGESLGLA
                     VGEWREVRTFAEVVLPCVALVKAPGGHRAANDEDTPAPLVPALLLKSDGTRFLYFRAG
                     SESGETAPVADFPLLFQPAMLSVGRAPQAANEAGAEEDKVEAQPRGFGFSWFVPELLR
                     HKSVWRDVLLASLAIQLMALATPLFTQAIIDKVIVHQTHSTLKVVAFALGMFMLFSAA
                     MTWVRQYLVIHTGNRIDAVLGTHVFSYMLRLPLRYFEQRPTGVVVSRLHGVETIRDFL
                     SGAAVSLVLDLPFLFIFVAVMFMYSWQLTLVALTALTIISLLSLAATPVFRKRLNDQF
                     LLGARNQAFVTEYVSGMATVKSMQMEPTLERRYGDYLASYLKAGFRARQLSNTINTLA
                     SAVEQGMTLSILVVGALLVMTNPGFTIGMLVAFQMFASRMSQPMLRLVGLWQEFQQAS
                     VAVKRLGDMMNIPTEPYATVPARSHRNAGRIDVISLGFRYSEQHPLIYRDFSMILRPG
                     QLTALMGPSGSGKSTLTKLLLGFYAQTDGQILLDGRDLRNLSANELRQAFGVVPQETL
                     LFSGTIYENLIMANPHAAFQDVIEACRKAEIHEVIEGLPQGYQTVIGEHGVGLSGGQR
                     QRVAIARALLKRPSVLIFDEATSNLDVETSEHFARTVNQLKGSTTVLYIAHQLPRGLA
                     VDAIVRLSDPSRPAVASDSAAPLRPLPPQMRTPAASAGDQQAL"
     gene            8959..14064
                     /locus_tag="VD791_07720"
     CDS             8959..14064
                     /locus_tag="VD791_07720"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HXV07901.1"
                     /translation="MATEYVTGTDANDWLDGGEGENEYQGGQGDDWINGATGENTIVY
                     NLGDGIDTISYAPPRSYQFAGWLEEIERALASDDFSSATYGNTYFGSLDSSLLQRLPS
                     GTSMANVLQALSTPLWDDDAGTLTPGTVDGAAARSAFQELLDWINTPVTNVVKFGPGI
                     SLADLSVQTSPDSSFGAPAIFAIAIGGGDQGVVFNMVPPDLAAASSMPSEPPPMDITF
                     EFVDENGQVTTATLAEILAQSDGGVAGFNNGTDAADVINGSLADDQIYGYGGDDLIDG
                     GAGMDFIYGGNGFGFGSGNDVIAGGAGSDIISGEDGDDIIAAGRDGGSVGGGAGNDVY
                     LFNLGDGALFVDNTPGIEGGETDTLSFGKDVAPENVIAYVDEFGTLTLAVQGTSDQVL
                     INWFMWDWGTSQWVVREDQVVPRVQFIDASGEARVYDLTALVTSNQTQLLGATVDSPV
                     FLFGGIDGSQYDLTGTVPVAGGDYATRYAITGDMFNDPPAGNQAPQVGTTIADVSAVE
                     GQPLTIALPQGAFTDADGDSLTYSAALDSGAGLVGLPTWLQFNSAMGTFTGTPDDAEV
                     AAGALRVVVTASDGQASASQTFGLTFVAVNDAPQAAAPNVFADAIEDAAFSLALPAGS
                     FLDVDSALTVSADPADMPAWLTFDAATMTFRGTPANGDVGSVSITVTATDGQYSAFTE
                     VTIGVANVNDDPTVINPMDDVSVTEDSDVAIDVSSVFGDVDLGDILSVSADTPAWLTY
                     TNGELWGTPGNGDVGTHSVTLTATDLAGGTVQHTFSVTVENVNDAPSVVNSVGDRSVN
                     EDSAIAVDVGNVFGDIDLGDSLSVSVSAPSWLTYSNGVLSGTPGNAEVGTHVVTLTAT
                     DVAGESVQEAFSITVLNLNDAPTVQNEIGNWVIDEDSAFSMTIPADVFADVDAGDSLT
                     VSLTGVPAWLTYDPATRVLSGMPGNADVGSYTLTLVADDGEATAEESFSITVANTNDA
                     PTREVEMADQVADEGSPVNFSLPAGMFADVDGDVLTLTASMADGTELPSWLSFDAANG
                     TFSGTPGWDDAGTLSITVTASDGSASASDTFDLRVADVNTAPTVANPIAPQTATEDSE
                     FSFTVPDDTFADAQGDSLVLTATLADGSPLPAWLSFHSPFAGTPSDGDVGTITIVVSA
                     TDSAGLTVSTSFNLSVANVNDAPAGSPTINGTANEGQTLTADASGISDADGLGTFSYQ
                     WLRDGSVISGATGSGYVLGDADVGGQMSVRVSYTDGYGTAESLTSASTGPVVGTEEPA
                     PDPEPVPEPVPGQTLIGGSGNNTLTGGDGDDVLDGRGGRDVLNGLGGDDTFRFYRDGT
                     ASRGASRRHNGSPGVSGTGETVSINGKRVSYDTFNGGDGYDTLLGTSGADAILLDVDR
                     SRRLSGIEMIDAGDGDDVVDLTSRRFDYGDVTIEGGSGNDTLWSSSGDDLLYGESGND
                     NLHGGAGDDYLYGGSGRDELRGGRGLDLLQGGSGDDELRDGGRGMLHGGSGNDLLVGG
                     SGNTLFVGGSGNDRIRLGGGNDVILYNSGDGRDVVESSGGSATLSLGGRIRISDLKFR
                     RSGQDLILETGNNESITFDDWYRGRQYRAVDTLQLITSGGMSGGSSEMRDDTVEMFDF
                     GDLVARYDRARRSNPGLSRWALTNGLMSFQLDASSDEAMGGDLAHQYGMTGSLAGITV
                     SAAQDVIGSSDFGSDTQRLRTLNLHEEGLVKLS"
     gene            14209..15582
                     /locus_tag="VD791_07725"
     CDS             14209..15582
                     /locus_tag="VD791_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HXV07902.1"
                     /translation="MKPSQPATGAEALDFSPAILRLQRQPPSPLPRTVVYLLGGLLAG
                     LLLWSVVGKLDIIAVAPGRLVPSSYVKVVQPADAGIIKAILVREGDLVQKDQVLVRMD
                     ASLSEADSRQVQTQLQVAALQLRRIDAELNGVEPTVQPEDPPEVFQQMAAQYRARRQA
                     QLDALATERAVLARTEQDLRGAQEQAQKLRKMLPIYVDQERSWRQLVDEGFAGRLQAE
                     ERKRLRIEAEQELKARQAAIEGYRATIQQSHQRLAQIESAYRQQLHNERVQIDAQYQR
                     LVQEWEKQLHRNELLELKAPQDGIIKDLATHTEGAVLQPGTVLMTIVPDNEPLRAEVW
                     VDNRDRGFVREGQAVKVKVAPYPFQKYGMVEGVVKTVSADASEAPQRGTPPTQGGNSN
                     GQSGGGYQFRSIVELKSQVLEFDGVRHKLMPGMQVDAEISLGERTVLEYLLSPVRKAF
                     HEAGRER"
     gene            complement(15659..17815)
                     /locus_tag="VD791_07730"
     CDS             complement(15659..17815)
                     /locus_tag="VD791_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07903.1"
                     /translation="MLERYSRVAALAVAVFLGAGHGGPAAPQDAGHGIRIGDTDLGGV
                     VSSENGPEAGVWVIAETSDLPTKFAKIVVTDDRGRYVIPDLPKASYSVWVRGYGLVDS
                     PKLRTAPGRIVDLRAVVAPSAAAAAEYYPAIYWYSMLEVPAKSEFPGTGPDANGMPVA
                     LKSQAQWLDVVKTNGCYTCHQLGNKATRTIPKELGHFDSSADAWARRIASGQAMTQMN
                     NNIGRLDARRALALFADWTDRIAAGALPGSQPTRPQGVERNVVITLWDWAGPRDYLHD
                     EISTDKRNPRVNANGLIYGATEESTDLFPVLDPVKHKATQLKMPVRDPSTPSSKRNPM
                     LPSPYWGPEPIWDSQASIHNPMFDEKGRVWFTSRVRPPENPDFCKKGSDHPSAKLTPV
                     ESSNRHLSMHDPKTGKFTLIGTCFPTHHLVFAEDADNTLWTSAGGPQSGVIGWLNRRM
                     FEETGDEAKSQGWTALVLDTNGNGKRDQHVEPAEAVDPSIDKRIAAAFYGVAVSPVDG
                     AVWGTVLGFPGYVIRLDPGRNPPATALAEVFEPPLPGYGPRGMDIDRSGVVWVPLSSG
                     HMGSFDRRKCKGPLNGPKATGRHCPEGWALYPFPGPQLRNVSDSGSAEASYYTWVDQF
                     DTFGLGRDVPMATGNANESLLALVDGKWINLRVPYPMGFYAKWMDGRIDDPNAGWKGK
                     GLWSTYATRTPFHVEGGKGTVSKVVKFQLRPDPLAR"
     gene            complement(17845..18930)
                     /locus_tag="VD791_07735"
     CDS             complement(17845..18930)
                     /locus_tag="VD791_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXV07904.1"
                     /translation="MSIARVQTDHYRIELPVTVSDSTHGQMSHFELITVRVHDTDGAQ
                     GTGYTYTVGCNGAAIHATVARDFAPLLQGQRADLIEALWQKLWWASHYGGRGGAAAMS
                     IAAVDIALWDLKARKLGVPLWTLLGGHDARVPCYAGGIDLHFTLERLLAQTDDNLKKG
                     FRAIKMKVGRHRLSEDVARVQAMREHLGADFPLMVDANMRWTPDEAVRAARAMRAFDV
                     YWLEEPTIPDDVEGHARVVREGGLPVATGENLHTLYEFKRMIAAGAVTFPEPDVTVCG
                     GVTGFIKVARLAEAFNLPVTSHGAHDITVHLLAAAPNRSYLEAHGFGLDRYIAHPLQI
                     ADGFAVAPDRPGHGVEFDWGGLERCRT"
     gene            complement(18986..19474)
                     /locus_tag="VD791_07740"
     CDS             complement(18986..19474)
                     /locus_tag="VD791_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07905.1"
                     /translation="MKTQWHQSGVTLVWWLVGILALAGLLLALWIWTMLSWSYSAGER
                     AGWVQKLSKKGWLCKTWEGEMAMVSLPGSVPEKFLFTVWDDGTADRINAVMGRRVSLF
                     YEEHILLPSSCFGETRHFVKDVRAIDEVPAPMIVPSVPGARPAPAPPADQGPPLEGPQ
                     TQ"
     gene            19674..>20372
                     /locus_tag="VD791_07745"
     CDS             19674..>20372
                     /locus_tag="VD791_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HXV07906.1"
                     /translation="MLSPPSAGRRNDWAVIRSLLPYLWEYKWRVGLALAFLVCAKLAN
                     VGVPLVMKEIIDSLDAQQALLALPLTLLAMYGLLRLSTTLFAELRDIVFVRVAKRAIR
                     RVALQVFRHLHSLSLRFHLERQTGGLSRAIERGTDGIEFLVSYLVFHIAPILLEIVLV
                     AAILWTYFNFGFAAVTFLVVVFYGVYTAAATQWRLKFRRAMNAQDSEANSRAIDSLLN
                     FETVKYFGNEKHELE"
BASE COUNT         3306 a         7001 c         6839 g         3226 t
ORIGIN      
        1 tagctgccgc ccgaaggcag ccgtgcggcc tggatcatct gatcgagcgt cgccggcgcg
       61 tagcgcgcct cgaccatctc gacgaactcg gtgaagacga tgcctttcat ggctgcgggc
      121 ggatgacctc cgctccacta tcggccccgc ctcgggggac ttgagcgccg ggcccgaccg
      181 cccgtcgcag cggaccgcag cggcgcgccc ctgcgaatgc acgccgggcg ttcgacacat
      241 tcgaacccgg gcctttgcga taatccaccc atgctgatgg aaaccctgca agccatgcgc
      301 gaccttccgc gcctgcatga gatcacgctg gtgctgatcc ggcacggctt cggcgacctg
      361 gtgcgccgcg cgggagtggc ggggctgctg gagcgtgcag ggcaggtgct gcgctgggga
      421 gcacgcgcgc aggtcgcgca gcttaccccg ccacagcgcg tgcgcatggc gctatccgag
      481 ctcgggccca ccttcgtcaa gctcggccaa gtcctcgcca cccgcgggga cctgttcccg
      541 ccggactgga tcgccgagtt cgagacgctg caggcgaatg tccccccggt tgccttcgac
      601 accttgctgc cggagatcga gcgggcgctg ggccgctcgc cgctggagct gtttcgcgag
      661 ctcgagcgcg agccgctggg cgccgcctcg atcgcgcagg tacacggcgc caagctgccc
      721 gacggcacgc cggtggtcgt gaaagtgatg cgccccggca tccgccccaa gatcgaggcc
      781 gatctgcgca tcctcggcca tctcgctgcc atggtggagg cggagatgcc cgaggcgcgc
      841 cgcttccagc cggtgcagat ggtcgcggag tttcgccgct ccatgcaccg cgagctcgat
      901 ctcgcgctcg aagcgcgcgc gcaggaacgc ttcgcgcaca acttcgcggg cgaccccaac
      961 gttctcatcc cgcgcatcta ctgggagtac acgcgcgaga cgctcaacgt gcagcaacgc
     1021 gtggtcggca ttccggggaa cgatctggca gcggtcgatg ccgccgggct cgaccgcgcg
     1081 ctgctcgccc ggcgcggggc cgatgcggtg ctgaagatga tcctgatgga cggctacttc
     1141 cacgcagatc cgcatccggg caacgtcttc tacctgccgg gcaaccgcct ggcgatgatc
     1201 gatttcggca tggcgggcag gcttaccgag cggcgccggc tgcagatcgt ggagatgctg
     1261 tgggcggtgg cgcggcgcga ggagtcgggc atcgtggagg tgctgctcga atgggtgagc
     1321 gacgccgaag tggacgaggc caggctggcg gcggacgtgg ccgagatgct gctcgactac
     1381 gcgcacctgg cgttgaagga cctgcggctg gggaccttgc tgcaggacat cgtaggcgtg
     1441 atacgccgcc attcgatcgt gctgccggcc gatctcgcgc tgctgttcaa ggcgctcatc
     1501 acgctcgagg ggctgggccg ccaactggat cccgagttcc gcctggtgga gcacctgacg
     1561 cccttcctca cgcgcctgat gcgcacgcga taccgcccgc gcgccctgct caaacgcggg
     1621 cgcgagcggt tgctggaaat cgcctcgctg gtgtcgagca tgcccgagga cctgcggcgg
     1681 ctggtgaagg aggcgcgccg cgggcgcatg aagatggagc tcgacctcaa gcgtctcgac
     1741 cacttcggcc atcagctcga ccgcagcgcc aaccggctga cggtgggcat catcaccgca
     1801 gccctgatcg tcggctcctc catcgtcatg accgtcgaag gcggcccaac cctgttcggc
     1861 ctgcccctgt tcggctttct cggcttcctg ctcgcattcg tggcggggat cgggctcgtc
     1921 gtgtcgatct ggcggtcccg gcgggagtaa gcggccgccc cgtcgcggcc gccgaaaaag
     1981 ggcgcacggt gcttgctttt cgccagtctc gaccctattc gaaggggata gcgcaacccg
     2041 aggcgcccgg gatccgggat agggcgggcc ctccggtata atccttggtt tttgcaagct
     2101 ttcgagggac acgatgccga tctacgaata ccgctgcggc agctgcggat ttgagaagga
     2161 gtatctgcag aagctgtccg atgcgcccat caccgactgc ccgacctgcg gcaagcccgc
     2221 gatgaccaag ctggtgtccg ccgcgggctt tcagctcaag ggcacaggct ggtacgcgac
     2281 cgacttcaag aacgggtcgc gcaaggctcc cggggcttca ggaaaaggcg gggcgcccgg
     2341 caagggcgac gcagaaggat ccgggaccgg agaggccaag aaggacacgc cgaaagccga
     2401 cgcggcgccg gcctgcggcg ccgggggctg cccggcctgc caataggcgc ggcgttgaaa
     2461 aagtacttcg tcaccggtct gctgatctgg atcccgctgg cgatcacggt ctggttgctg
     2521 aacctgatcg tgacgaccat ggaccagacg ctctggctgc tgcccgcgtc cctgcacccg
     2581 agcgcctggc tgggcgtgca catccccggc ctggggctgc tgctgtcgct gctggtcgtg
     2641 ctgctcaccg gcgtgctggc cgccaacatc atcggccagc ggctggtgca gatctgggag
     2701 gcgatgctgg cccgcatccc ggtggttaag tcgatctaca acagcgtcaa gcaggtctcc
     2761 gacacactgc tctccagcac cggcgaggcg tttcgcaagg ccgtgctggt gcaatatccg
     2821 cgcgagggcg cttggaccat cgccttcctg accggcgtgc ccgggggcga cgtggccacc
     2881 cacctgcgcg gagactatgt gagcgtgtac gtgccgacca cgccgaaccc gacttccggt
     2941 ttcttcctga tgttgccgcg ggcggaggtg atcgagctga acatgagcgt ggatgcggcc
     3001 ctcaagtacg tcatctccat gggggtcgcg gggcccgcgc cgccggcccg caaggccccg
     3061 gcgcccaagc cggtcgtaac gccctgagcc tgcgagccga taccgccccg cgggcgcggc
     3121 gcctgcggtg ataaccattc gaacccatcc tctcgcctac ccgatgcgca ccgactactg
     3181 cgggctgatc gacaaacgcc acctcggccg gaacgtcacg ctgttcggct gggtccaccg
     3241 ccgccgcgat cacggcggcg tgatcttcat cgacctgcgc gatcgggaag gactggtgca
     3301 ggtggtggtc gatcccgaca cgccggagac cttcgccacc gcggaccgcg cgcgcggcga
     3361 atacgtgctc agggtcgaag gccgcgtgcg gccgcgcccg gccggcaccg tcaacccgaa
     3421 cctggtgagc ggcgaagttg aagtgctggc gaaggacatc gaagtgctca acgcctcgct
     3481 cacgccgccg ttccagctgg acgaggacgg gctcggcgaa aacgtgcgcc tggagcaccg
     3541 cgtgatcgac ctgcgccgcc ccgagatgca gcgcaatctg cggctgcgct accgggtggc
     3601 gatggcagtg cgccgccacc tcgacgccga gggcttcctc gacatcgaga cgccgatcct
     3661 caccaagtcc acgcccgagg gggcgcgcga cttcctggtg cccagccgca tgcacgccgg
     3721 ggagttctac gccttgccgc agtcgccgca gctgttcaag cagctgctga tgatcgccgg
     3781 cttcgaccgc tactaccaga tcacgcgctg ctttcgcgac gaagacctgc gcgccgaccg
     3841 ccagcccgag ttcacccaga tcgacgtcga gacctcgttc ctgggcgtgc ccgagatcac
     3901 cgatctgatg gagcgcatga tcgtcgcggt gttcaaggag gtgatggcgg tcgatctacc
     3961 cactccgttc ccgcgcatca cctacgcgga ggcgatgtcc aggtacggct cggacaagcc
     4021 cgacctgcgc gtgccgctca cgctcaccga gctgaccgac gtgatgaagg gggtcgagtt
     4081 caaggtgttc agcgctgcgg ccaattcggc gggagggcgg gtcgcggcgc tgcgcgttcc
     4141 gcgcggcggc gagctcacgc gcagcgagat cgacgcctac ggtgagttcg tcgccatcta
     4201 cggcgccaag gggctcgcct acatcaaggt caacgacgcc ggccgcggcc gggacggcct
     4261 gcaatcgccg gtggtcaaga atctgtccga tgccgcgctg caggcgatcg tgcagcgcac
     4321 cggcgccgag aacggcgacc tgatcttctt cggcgccgac aaggcgaagg tggtgaacga
     4381 atcgctcggg gcgctgcgcg ccaagctggg gcacgagcgc ggcttcgtgc aggccggctg
     4441 gcgaccgctg tgggtgctgg agttcccgat gttcgagtgg aacgaggagg agaagcgctg
     4501 ggacgcgctg caccacccgt tcacctcgcc catggagggc cacgacaact ggctcgacgg
     4561 cgatcccggc aaggcgctgt ccaaggccta cgacatggtg ctcaacggct gggaaatcgg
     4621 cggcggctcg gtgcgcatcc accgccagga agtgcaggcc aaagtgttct cggcgctcaa
     4681 catccgcccc gaggaggcgc gtgccaagtt cggattcctg ctcgacgcgc tgcaatacgg
     4741 cgccccccct cacggaggga tcgccttcgg gctggaccgc atcgtcgcgc tgatggccgg
     4801 cacgcagtcg atccgcgatg tgatcgcgtt ccccaagacg cagcgcggcc agtgcctgct
     4861 cacccgggcg cccagcccgg tggacgagcg ccagctgcgc gagctgcaca tccgcgtgcg
     4921 cgcgccggag aagcagtagc cacagagaac acagaggtca cagagcgaaa tgcgtgggga
     4981 ggcgatcgag cccaagaatg cggcgcaccg atgatgcagc cagtgcccgc cctgagctct
     5041 ctgtgatctc tgtggccacg cagtacaaga ttccgcgatc ggtgctggtc gtggtgcaca
     5101 cgtccgacct gcaggtcctg ctgctggagc gcgccgacca cccgggcttc tggcagtcgg
     5161 tcaccggcag ccagcacgaa ggcgagacgc tggagcagac cgcggtgcgc gaggtggcgg
     5221 aggagaccgg cctggacgcg gcccgccacc ggctgctcga ctggggcatg cgcaacgact
     5281 acgagatcta ccgcgagtgg cgctggcgtt atgcgcctgg cgtcacgcac aacaccgagc
     5341 acgtcttcgg cctcaccgtg ccgcagccga tcgacgtgcg catcgccccg cgcgagcacc
     5401 tcgcgttcgt ctggctgccc tggcgcgaag cggccgacaa gtgcttctcc tggagcaacg
     5461 cgcaggccat ccgcaatctg cccgaaaagg taagctaggc gctcgaagcg cgaagcaccg
     5521 aaccatgccg aacctgcacg tcctcaccta caatatccac aagggattcc cgcaattcaa
     5581 ccggcgcgtc tcgctgcacg agctgcgcga ccgcctgcag tcggtcgagc cggacctcgt
     5641 gttcctgcaa gaggtggtcg gtttccatgc gcgccacgcc gagcgccatg ccgagtggcc
     5701 gacctcgccc cagtacgagt tcctcgccga ccaggtgtgg cgcgatttcg cttacggccg
     5761 caacgcggtg gtggacgacg cgcaccacgg caacgccatc ctgtccaagt tcccgatcac
     5821 gcgctgggac aacgaggacg tctccgccaa tcagctcgag cggcgcggcc tgctccactg
     5881 cgagatcgag attcccggct gggaccggcg cctgcacgcc atcaacgtgc acctgggcct
     5941 gctgcgcgtg tggcgtgacc gccaactgct ggccctggtg catcgcatcg agcggctggt
     6001 gccgcacgat gcgcccctga tcgtggccgg cgacttcaac gactggacgc gcaaggcgag
     6061 ccgcctgctg atgcgctcgc tggacgtgag cgaagtgttc gagacctacg gccacggccc
     6121 cgcgcgcagc tttcccgcca tgctgccggt gctgcacctg gaccgcatct acgtgcgcgg
     6181 cttccgcatc aagctcgccc acgtccaccg cgggttgcac tggtcgcgca tctccgacca
     6241 cgccgcgctt tccgccctgc tgatgcgcga acaggcataa gggttgctac ggggatgggt
     6301 tgttctttcc ccccgacctg cgcacgtgtt ccccttgccc aagcggcgct cagtgccggc
     6361 tgcgccgcgt gaagaagccg ccatcctggc cacgcccatc gagccggctc cgatgctttg
     6421 ggtgctgggc agcctgtgcc agatctaccg catcccgttc gacgccaagc tggtcctgca
     6481 gcgcttcgcg ccgccctata cgctggcgac cctgtgcgag gcgggcgagt ccctgggcct
     6541 cgcggtcgga gagtggcgcg aagtgcggac cttcgccgag gtggtgctgc cttgcgtggc
     6601 cctggtcaag gccccgggcg ggcaccgcgc ggccaacgac gaggacacgc ccgctccgct
     6661 ggtgccggcc ctgctcctga agagcgacgg cacgcgcttt ctctatttcc gggcgggatc
     6721 ggagtcgggc gaaaccgcgc cggtggccga ctttccgctg ctgttccagc ccgccatgct
     6781 gtcggtcgga cgggctccgc aggcggccaa cgaggccggc gccgaagaag acaaggtcga
     6841 ggcccagccg cgcggcttcg ggttctcctg gttcgttccc gagctgctca ggcacaagag
     6901 cgtgtggcgc gacgtgctcc tggcctcgct cgccatccag ctgatggcgc tcgccacgcc
     6961 cttgtttacg caggcgatca tcgacaaggt gatcgtgcac cagacgcaca gcacgctgaa
     7021 ggtggtggcg ttcgcgctgg gcatgttcat gctgttctcg gcggcgatga cctgggtacg
     7081 gcaatacctg gtgatccaca ccggaaaccg catcgacgcg gtgctcggca cgcacgtgtt
     7141 ctcctacatg ctgcggctgc ccctgcgcta cttcgagcag cgccccaccg gggtggtggt
     7201 gtcgcggctg cacggggtgg agacgatccg cgacttcctc tccggcgcgg cggtgtcgct
     7261 ggtgctcgac ctgccgttcc tgttcatctt cgtggcggtg atgttcatgt acagctggca
     7321 gctcacgctg gtggcgctca ccgcgctcac catcatctcc ttgctgagcc tggccgccac
     7381 gccggtgttc cgcaagcggc tcaacgacca gttcctgctg ggcgcgcgca accaggcgtt
     7441 cgtcaccgag tacgtgagcg gcatggccac ggtcaagagc atgcagatgg agccgacgct
     7501 ggagcggcgc tatggcgact acctggcgag ctacctcaag gccggcttcc gggcacggca
     7561 actgtccaac accatcaaca cgctggccag cgccgtcgag cagggcatga cgctgtccat
     7621 cctggtggtg ggggcgctgc tggtgatgac caaccccggc ttcaccatcg gcatgctcgt
     7681 cgcgttccag atgttcgcca gccgcatgtc gcagccgatg ctgcggctgg tcggcctgtg
     7741 gcaggagttc cagcaggcct cggtcgcggt caagcgcctg ggcgacatga tgaacatccc
     7801 gaccgagccg tacgccaccg tgcccgcccg cagccaccgc aacgccggcc gtatcgacgt
     7861 gatttcgctc ggtttccgct acagcgaaca gcacccgctg atctaccgcg acttcagcat
     7921 gatcctgcgc cccggccagc tcactgcgct gatggggccg tcgggctcag gcaagagcac
     7981 gctcaccaag ctgctgctcg gcttctatgc ccagaccgac ggccagatcc tgctggacgg
     8041 gcgcgatctg cgcaacctgt cggccaacga gctgcgccag gctttcggcg tggtgcccca
     8101 ggagacgctg ctgttctccg gcaccatcta cgagaacctg atcatggcca atccgcacgc
     8161 cgccttccag gacgtgatcg aggcctgccg caaggcggag atccacgagg tgatcgaggg
     8221 cctgccgcag ggctaccaga cggtgatcgg ggagcacggc gtcgggctgt cgggcgggca
     8281 gcggcagcgc gtcgccatcg cccgggcgct gctcaaacgc ccttccgtgc tgatcttcga
     8341 cgaagccacc agcaacctgg acgtggaaac cagcgagcac ttcgcccgca ccgtcaatca
     8401 gctcaaggga tccaccactg ttctttatat cgcccaccag ctcccacgcg ggctggcggt
     8461 ggacgccatc gtgcgcctga gcgatccctc gcgtcccgcc gttgcctcgg acagcgccgc
     8521 gccgctgcgc ccgctgcctc cgcagatgcg cacgcccgcg gcatccgcgg gcgatcagca
     8581 ggcgctgtga cgcgtcgttg acacttccgt cgctcttgcc catccgcggc aggagtgacg
     8641 gcgtgcggcg gccgccaaga gcgttctcgt aacgcttatt cacttcttcg caacgatccg
     8701 cgacgcatgc cggcgacgtc cgccgggtag tcttgcgcca acgtcacagt cagctttcag
     8761 cactgtcatg gcggcggcga tcttcaccat ggttcacaga ttcagtcact tagtggcatg
     8821 caccttgcgc cctgtcgggc gtaagcaaca gtttttggcc ttccggggcg agaaccggag
     8881 gcgtcgcaat gcgcccgtga gcgaactcac gaggtgctgg cagacgcaaa tcgtgcaaag
     8941 gcgcagctgg agacgaagat ggctactgaa tacgtgaccg gaaccgacgc aaacgactgg
     9001 ctcgacggcg gcgaaggcga gaacgaatac cagggcggtc agggcgacga ctggatcaat
     9061 ggcgccaccg gcgagaacac catcgtctac aacctgggtg acggcatcga cacgatcagc
     9121 tatgcgccgc cgcgcagcta tcagttcgcc ggctggctcg aggagatcga gcgcgcgctg
     9181 gcgagcgacg atttctccag tgccacctac ggcaacacct acttcgggag cttggactct
     9241 tccttgttgc agcggctgcc gtccggaacg agcatggcca acgtgctgca ggcgctcagc
     9301 accccgctgt gggacgacga cgccggcacg ctgacgcccg gcacggtgga cggcgccgcc
     9361 gcgcgctccg ctttccaaga actcctggac tggatcaaca cgcccgtcac caacgtggtc
     9421 aagttcggcc cgggcatctc tctcgccgat ctgagcgtgc agaccagccc cgacagcagc
     9481 ttcggggcgc cggcgatctt cgcgatcgcc atcggcggcg gcgatcaggg ggtggtgttc
     9541 aatatggtgc cgcccgacct ggcggccgct agcagcatgc cgagcgaacc accgcccatg
     9601 gacatcacct tcgagttcgt cgacgagaac gggcaggtca cgaccgctac gctggcggaa
     9661 atcctcgccc agtccgacgg cggtgtggcg ggcttcaaca acggcacgga cgcggccgat
     9721 gtcatcaacg gcagcctggc cgacgaccag atctacggct acggcggcga cgacctgatc
     9781 gacggcggcg ccgggatgga cttcatctat ggcggcaacg gcttcggctt cggcagcggc
     9841 aacgacgtga tcgccggcgg cgcgggcagc gacatcatct cgggcgagga cggcgacgac
     9901 atcatcgccg ccgggcgcga cggcggttcg gtgggcggcg gcgccggcaa cgacgtatat
     9961 ctgttcaacc tcggcgacgg cgccctgttc gtggacaaca ccccgggcat cgaaggcggt
    10021 gaaaccgaca cgctttcctt cggcaaggac gtcgccccgg aaaacgtcat cgcctatgtg
    10081 gacgagttcg ggaccctgac cctggcggtg cagggcacct ccgaccaggt gctgatcaac
    10141 tggttcatgt gggattgggg tacctctcaa tgggtcgtgc gtgaggacca ggtggtgccg
    10201 cgcgtgcagt tcatcgacgc ctcgggcgag gcgcgcgtgt acgatctgac cgcgctggtg
    10261 accagcaatc agacccagct gctcggcgcg acggtggaca gccccgtgtt cctgttcggg
    10321 ggcatcgacg gcagccagta cgacttgacg ggcaccgtcc cggtggccgg tggcgactac
    10381 gccacgcgct atgcgatcac cggtgacatg ttcaacgatc caccagcggg taaccaggcg
    10441 ccgcaggtcg gcaccaccat cgctgacgtc agcgcggtcg aagggcagcc gctgacgatc
    10501 gcgctgcctc agggcgcgtt cacggacgcc gacggcgaca gcctcaccta ctcggccgcg
    10561 ctggacagcg gcgcggggct ggtgggcctg ccgacctggc tgcagttcaa ctcggcgatg
    10621 ggcaccttca ccggcacgcc ggacgacgcc gaggtggcgg ccggcgctct gcgagtcgtg
    10681 gtcacggcct ccgacgggca agcgtcggct tcgcagacct tcgggctgac cttcgtcgcc
    10741 gtgaacgatg cgccccaggc cgccgcgcca aacgtgtttg cggacgccat cgaggacgcc
    10801 gccttcagcc tggcgctgcc ggcgggctcg ttcttggatg tcgacagcgc gctgacggtg
    10861 agcgccgacc cggccgacat gccggcgtgg ctcaccttcg atgccgccac catgaccttc
    10921 aggggcacgc ccgcgaacgg cgatgtgggc agcgtctcga tcacggtgac cgccaccgac
    10981 ggccagtaca gcgcgttcac cgaagtgacc atcggcgtgg ccaacgtcaa cgacgacccg
    11041 acggtgatca acccgatgga cgacgtgagc gtcaccgagg acagcgacgt ggccatcgac
    11101 gtgagcagcg tgttcggcga cgtcgatctg ggcgacatcc tcagcgtgag cgccgacact
    11161 ccggcgtggc tgacttacac caacggcgag ctgtggggca caccgggcaa cggcgacgtc
    11221 ggcactcact cggtgacact gacggcaacg gatctggccg gcgggaccgt gcagcatacc
    11281 ttcagcgtca cggtcgagaa cgtcaatgat gcgccgagcg tggtcaactc cgtgggcgat
    11341 cgcagcgtga acgaggactc cgcgatcgcc gtcgatgtgg gcaatgtgtt cggcgacatc
    11401 gacctcggcg acagcctgag cgtgagcgtg tccgctccgt cgtggctgac ctacagcaac
    11461 ggcgtcctgt ccggcacgcc gggcaatgcc gaggtgggca cccacgtggt gacgctgacc
    11521 gcgaccgatg tggccgggga aagcgtgcag gaggccttca gcatcacggt gctgaacctc
    11581 aacgacgcgc ccaccgtgca gaacgaaatc ggcaactggg tgatcgacga ggacagcgcg
    11641 ttctcgatga cgatcccggc cgacgtcttc gccgacgtgg atgccggcga ttcgctgacc
    11701 gtgagcctga ccggcgtgcc ggcatggctg acctacgacc cggctactcg cgtgctgtcc
    11761 ggcatgccgg gcaacgccga cgtgggcagc tacacgctca cgctggtggc cgacgacggc
    11821 gaagcgaccg ccgaggaatc tttcagcatc accgtggcga acaccaacga tgcgcccacc
    11881 cgcgaggtcg agatggccga ccaggttgcc gacgaaggca gcccggtcaa cttctcgctg
    11941 ccggccggca tgttcgccga cgtcgacggc gatgtgctca cgctgacggc cagcatggcc
    12001 gacggcacgg agctgccgag ctggctcagc ttcgacgccg ccaacggcac cttcagcggc
    12061 acgcccggct gggacgatgc gggcacgctg agcatcacgg tcaccgcgag cgacggttct
    12121 gccagtgcgt cggacacctt cgatctgcgt gtcgccgacg tcaacacggc gcccacggtg
    12181 gccaacccga tcgcgcctca aacggcgacc gaagacagcg agttcagctt caccgtgccg
    12241 gacgacacgt tcgccgacgc gcagggcgac tccctggtgc tgaccgcgac gctggccgat
    12301 ggctcaccgc tgcccgcgtg gcttagcttc cattcgccct tcgccggcac cccgagcgac
    12361 ggcgacgtgg gcacgatcac gatcgtcgtc agcgcaaccg atagtgccgg cctgaccgtc
    12421 tcgaccagct tcaacctcag cgtggccaac gtcaacgatg cgccggcggg gtcgccgacg
    12481 atcaacggaa cggccaacga gggtcagacc ctgacggcgg acgcctccgg catcagcgat
    12541 gccgacgggc tgggcacctt cagctatcaa tggttgcgtg acggcagcgt gatttccgga
    12601 gcgaccggta gcggctacgt cctgggcgat gccgatgtcg gcggccagat gagcgtgcgc
    12661 gtgtcgtata ccgatggtta cggcaccgcg gagtcgctga cttcggcgtc gaccgggccg
    12721 gtggtgggta cggaagagcc tgcacccgat ccagaaccgg tacccgagcc cgtacccggc
    12781 cagaccctca tcggcgggag cggcaacaac acgctgactg gcggcgacgg cgacgacgtg
    12841 ctggatggcc gcgggggccg ggatgtgctc aatggcctcg gcggagacga caccttccgc
    12901 ttctaccgcg atggcacggc gagccggggc gcgtcgcgca ggcacaacgg cagcccgggc
    12961 gtatccggca cgggcgagac cgtgagcatc aacggcaaac gcgtgagcta cgacaccttc
    13021 aacggcggcg acggctacga caccttgctc ggcaccagcg gggccgacgc catcctgctc
    13081 gatgtcgatc gcagccggcg cttgtcgggc atcgagatga tcgatgccgg tgacggcgac
    13141 gacgtggtcg atctgaccag ccggcgcttc gactacggcg acgtcaccat cgagggcggc
    13201 tccgggaacg acacgctgtg gtcgtccagc ggcgatgatt tgctgtacgg tgaatcgggc
    13261 aacgacaacc tccacggcgg cgccggcgac gactatttgt acggcggcag cggccgcgac
    13321 gagctacgcg gcggccgggg cctggacctg ctccagggcg gcagcggcga cgatgagttg
    13381 cgcgacggcg ggcggggcat gctccacggc ggcagcggca acgacctgct ggtgggcggc
    13441 tcgggcaaca ccctgttcgt gggcggcagc ggcaacgacc gcatccgcct gggcgggggc
    13501 aacgacgtga tcctctacaa cagcggcgac ggccgcgacg tggtggagag cagtggcggc
    13561 agcgccacgc tgtccctggg cgggcgcatc cgcatctccg acctcaagtt ccggcgctcc
    13621 ggccaggacc tgattctgga gaccggcaac aacgagtcga tcaccttcga cgactggtac
    13681 cgcggccggc aataccgggc ggtggacacc ctgcagctga tcaccagcgg cggcatgtcc
    13741 ggcgggtcga gcgagatgcg cgacgacaca gtcgagatgt tcgacttcgg cgacctggtg
    13801 gcgcgctacg accgtgcccg caggagcaac ccgggcctgt cccggtgggc gctgaccaac
    13861 gggctgatga gcttccagct cgacgccagc agcgacgagg cgatgggcgg cgatctggcc
    13921 caccagtacg ggatgaccgg gtctctggcc gggatcacgg tgagcgcggc ccaggacgtg
    13981 atcggctcgt ccgacttcgg cagcgacacg cagcggctgc gcactctgaa cctgcacgag
    14041 gaagggctgg tgaagctgtc ctgaaacggg cagtgaacgc cccgcgaggg gggttcaccc
    14101 ccccgttcgg gggagaggtc cggccttcag gggactggcg ccgatcccaa acttctttag
    14161 catgatcctt gcaggcgtgc cgcgcatgcc tgcaaggatc atttccccat gaagccttcg
    14221 cagcccgcca cgggcgccga ggcgctcgat ttctcccccg cgatcctgcg gctgcagcgc
    14281 cagccgccct cgccgctgcc gcgcacggtg gtgtacctgc tggggggcct gctggcgggg
    14341 ctgctgctgt ggtcggtggt cggcaagctc gacatcatcg ccgtggcgcc cgggcgcctg
    14401 gtgcccagca gctacgtcaa ggtggtgcag ccggccgacg ccggcatcat caaggcgatc
    14461 ctggtgcgcg agggcgacct ggtgcagaag gaccaggtgc tggtgcgcat ggatgccagc
    14521 ctgtccgagg ccgacagccg ccaggtccag acccagctgc aggtcgcggc actgcagctg
    14581 cgccgcatcg atgccgagct gaacggcgtg gagcccacgg ttcagccgga agatcctccc
    14641 gaggtgttcc agcagatggc ggcgcagtac cgcgcgcgcc gtcaggcgca actcgacgcg
    14701 ctggccaccg agcgcgccgt gctcgctcgc accgagcagg atctgcgcgg ggcgcaggag
    14761 caggcgcaga agctgcgcaa gatgctgccg atctacgtgg accaggagcg ctcctggcgc
    14821 cagctggtgg acgaaggttt tgccggccgg ttgcaggccg aggagcgcaa acgcctgcgc
    14881 atcgaggccg agcaggagct gaaagcccgc caggcggcga tcgagggcta ccgcgccacg
    14941 atccagcagt cgcaccagag gctggcccag atcgagtccg cctaccgcca gcagctgcac
    15001 aacgagcgcg tgcagatcga cgcgcagtat cagcgcctgg tgcaggaatg ggagaagcag
    15061 ctgcaccgca acgagctgct cgagctgaaa gcaccgcagg acggcatcat caaggacctg
    15121 gccacgcaca ccgagggcgc ggtgctgcag ccgggcacgg tgctgatgac catcgtgccc
    15181 gacaacgagc cgctgcgcgc ggaggtgtgg gtggacaacc gcgaccgcgg cttcgtgcgc
    15241 gaaggccagg cggtcaaggt gaaggtggcg ccctatccgt tccagaagta cggcatggtg
    15301 gaaggcgtgg tgaagacggt gagcgcggac gccagcgagg cgccgcagcg cggcaccccg
    15361 ccgacccagg gcggcaactc caacggccag agcggtggcg gctaccagtt ccgcagcatc
    15421 gtcgagctga aatcccaggt gctcgagttc gacggcgtgc ggcacaagct catgcccggc
    15481 atgcaggtcg acgccgagat ctcgctcggg gagcgcaccg tcctggagta cctgctgtcg
    15541 ccggtgcgca aggccttcca cgaggccggc cgcgagcgct gagcccgcgc gcaacgatcg
    15601 tcggagagcc cgccagccac gcgacaccct gggtacaggc cctcggcaag cgccggcttc
    15661 aacgcgcgag cggatccggg cgaagctgga acttcacgac cttgctgacg gtgcctttgc
    15721 cgccctcgac gtggaacggc gttcgcgtgg cataggtcga ccacagtccc ttccccttcc
    15781 agccggcatt cgggtcgtcg atgcgtccat ccatccattt cgcatagaag cccatgggat
    15841 agggcacgcg caggttgatc cacttcccgt ccacgagcgc cagcagcgac tcgttggcat
    15901 tgccggtggc catgggcacg tccctgccca gcccgaacgt gtcgaactga tccacccacg
    15961 tgtagtagct cgcttcggcg ctgccggagt cgctcacgtt gcgcagctgc ggtccgggaa
    16021 acggatagag cgcccaaccc tcgggacaat gccggccggt cgccttcggt ccgttgagcg
    16081 gccccttgca cttgcggcgg tcgaagctcc ccatatgccc gctggacagc ggcacccaca
    16141 ccacgccgct gcgatcgatg tccatgccac gcggcccgta gccgggcagc ggcggttcga
    16201 acacctccgc cagcgcagtt gccgggggat tgcggcccgg gtcgagccgg atgacgtaac
    16261 cggggaagcc cagcaccgtg ccccagacgg cgccgtccac cgggctcacg gcaacgccgt
    16321 agaacgcggc cgcgatgcgc ttgtctatgc tcggatccac cgcctccgcg ggttcgacat
    16381 gctggtcgcg cttgccgttg ccgttggtgt cgaggacgag cgctgtccag ccctgcgact
    16441 tcgcctcgtc gcccgtttcc tcgaacatcc tgcggttcag ccagccgatg acaccgctct
    16501 gcgggccgcc ggcgctggtc cacagcgtgt tgtcggcgtc ctccgcgaac accagatgat
    16561 gcgtgggaaa gcacgtgccg atcagcgtga acttcccggt cttgggatcg tgcatggaca
    16621 gatgccgatt ggacgattcg accggagtca gcttcgccga aggatgatcc gaccctttct
    16681 tgcagaagtc cgggttctcc ggcgggcgca cgcgcgaggt gaaccacacc cggcccttct
    16741 cgtcgaacat cgggttgtga atgctggcct ggctgtccca gatcggctcg ggaccccagt
    16801 acggcgaggg cagcatcgga ttgcgcttcg aggacggtgt gctcggatcg cgcaccggca
    16861 tcttgagctg cgtcgccttg tgcttcaccg ggtcgaggac cgggaacaga tccgtgctct
    16921 cttcggtggc accgtagatc aggccgttgg cgttcaccct cgggttgcgc ttgtcggtcg
    16981 agatctcgtc gtgcagataa tccctcggcc cggcccagtc ccagagcgtg atgaccacat
    17041 tgcgctccac gccttggggg cgcgtcggct gcgagccggg cagcgcgccg gccgcgatcc
    17101 gatccgtcca gtccgcgaac aaggcgagcg cccgccgagc gtcgagccgg ccgatgttgt
    17161 tgttcatctg ggtcatcgcc tgccccgatg cgatgcgccg cgcccaggca tcggccgagg
    17221 agtcgaagtg gcccagctcc ttggggatcg tgcgcgtcgc cttgttgccg agctggtgac
    17281 aggtgtagca gccgttggtc ttcaccacat ccagccactg cgcctggctc ttcagcgcca
    17341 ccggcatgcc gttggcgtcc ggtccggtgc cgggaaactc gctcttggcc ggtacctcga
    17401 gcatcgaata ccagtagatc gccggatagt attccgccgc ggccgctgca ctcggcgcca
    17461 ccaccgccct caagtcgacg attctgccgg gcgcggttcg cagcttcggc gagtcgacca
    17521 gcccgtagcc gcgcacccac acgctatagc tggctttcgg gagatcggga atgacatagc
    17581 gcccgcgatc gtcggtgacc acgatcttgg cgaacttggt cggaaggtcc gaggtctccg
    17641 cgatcaccca gacgccggct tccggcccgt tctcgctgct caccacgccg ccgaggtccg
    17701 tgtcgccgat gcggatccca tgcccggcgt cttgcggcgc ggccggtccg ccatggccgg
    17761 cgcctagaaa cacggcgacg gcgagcgccg ccactctcga gtaccgctca agcatggact
    17821 tcctccctcc agacgagccg acgctcaagt ccggcagcgc tccaacccgc cccagtcgaa
    17881 ctcgacgccg tgccccggcc gatcaggcgc cacggcgaac ccgtcggcga tttgcagcgg
    17941 gtgggcgatg tagcgatcca ggccgaagcc gtgcgcctcg aggtaagacc ggttgggtgc
    18001 cgcagcgagc aggtgcacgg tgatgtcgtg cgcgccgtgg gaggtcaccg gcaggttgaa
    18061 ggcttccgcc agccgggcca ccttgatgaa gccggtgacg ccgccacaca ccgtcacatc
    18121 cggctcggga aacgtcaccg cccccgccgc gatcatgcgc ttgaactcgt acagcgtgtg
    18181 caggttctcc ccggtggcca ccggcaggcc gccctcgcgc accacgcgtg catggccctc
    18241 gacgtcgtcg gggatggtgg gctcctccag ccagtacacg tcgaacgcgc gcatcgcccg
    18301 cgcagcccgc accgcctcgt ccggggtcca gcgcatgttc gcatccacca tcagcggaaa
    18361 gtccgcgccc aggtgctcgc gcatggcttg cacgcgcgcc acgtcctcgg acagtctgtg
    18421 ccggccgacc ttcatcttga tggcgcgaaa ccccttcttc agattgtcgt cggtctgcgc
    18481 cagcagccgc tccagcgtga agtgcagatc gatgccgccg gcgtagcagg gtacgcgtgc
    18541 gtcgtgcccg cccagcaggg tccacagcgg cacgcccagc ttgcgcgcct tcaggtccca
    18601 cagcgcgatg tccaccgcgg cgatggacat ggcggcggcc ccgccgcgcc cgccgtagtg
    18661 gctggcccac cacagcttct gccacagcgc ctcgatcagg tcggcgcgct ggccctgcag
    18721 cagcggcgcg aagtcgcgcg ccacggtggc gtggatggcg gcgccattgc agccgacggt
    18781 gtaggtgtaa cccgtgcctt gtgcgccgtc ggtgtcgtgc acgcgcacgg tgatcagctc
    18841 gaaatgactc atctgcccgt gggtgctgtc ggacaccgtc acgggcagtt cgatgcggta
    18901 gtggtcggtc tgtacgcggg cgatgctcat gggatggcgc tcgaatcgct gggtcgacgg
    18961 gcggcgcgtg ccgcggcggc cgatgtcact gggtctgcgg cccctcaagc ggcggccctt
    19021 gatcggccgg cggcgccgga gcgggccgcg cgcccggcac cgaagggacg atcatcggcg
    19081 ccgggacctc gtcgatggcg cgcacgtctt tcacgaagtg ccgcgtttcg ccgaagcagc
    19141 tgctgggcag caggatgtgc tcttcgtaga acagcgacac gcgccggccc atcaccgcgt
    19201 tgatccgatc cgcggtcccg tcgtcccaca cggtgaacag gaacttctcg ggcaccgagc
    19261 cgggcagcga taccatcgcc atctcgcctt cccaggtctt gcacagccag cccttcttcg
    19321 acagcttctg cacccagccg gcccgctcgc cggcggagta gctccaggac aacatggtcc
    19381 agatccacag cgcgagcagc aggccggcca gcgccaggat tcccaccagc caccagacca
    19441 gcgtgacacc gctttgatgc cattgcgttt tcaaggtcac gtcctcccgg tcgagccggc
    19501 attataggag ttcgcggtcc gctgcagaat cgccctggga gcgccgccgc atatcggccc
    19561 ggccgtcggg ctagaatgcg gacgtgctct caccaccgcc cgccacgacc cgctgcgcgc
    19621 tgccttggaa ccgcgccgcc ccccggcgcg accgctgatc gatgcatcac tccatgcttt
    19681 caccgccgag cgcgggccgc cgcaacgact gggcggtgat ccgctcgctg ctgccctacc
    19741 tgtgggaata caagtggcgg gtgggactgg cgctcgcctt cctggtctgc gccaagctgg
    19801 ccaacgtcgg cgtgccgctg gtgatgaagg agatcatcga ctcgctggac gcgcagcagg
    19861 cgctgctcgc gctgccgctg acgctgctcg cgatgtacgg gctcctgcgc ctgtccacca
    19921 cgctgttcgc ggagctgcgc gacatcgtgt tcgtgcgcgt ggccaagcgc gcgatccggc
    19981 gcgtggcgct gcaggtgttc cgccacctgc actcgctgtc gctgcgcttc cacctggagc
    20041 gccagaccgg cggcctctcg cgcgcaatcg agcgtggaac cgacggcatc gagttcctgg
    20101 tgtcgtatct cgtgttccac atcgcgccga tcctgctcga gatcgtgctc gtcgcagcca
    20161 tcttgtggac ctatttcaat ttcggcttcg ctgcggtcac gttcctggtc gtcgtgttct
    20221 acggcgtcta cacagcggcc gcgacacagt ggcggctgaa attccgccgt gcgatgaacg
    20281 cccaggacag cgaggccaat tcgcgagcga tcgacagcct gctcaatttc gagaccgtca
    20341 agtatttcgg caacgagaag cacgagctcg ag
//
LOCUS       DASPGE010000240         8548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_180212, whole genome shotgun sequence.
ACCESSION   DASPGE010000240 DASPGE010000000
VERSION     DASPGE010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8548
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180212"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..385)
                     /locus_tag="VD791_07750"
     CDS             complement(38..385)
                     /locus_tag="VD791_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXV07907.1"
                     /translation="MTRTIAREELARRIDSGKPPVLLEALPERYYAQKHLPGALLFPH
                     DQAAQLAPSVVPDRSAQIVVYCASRTCQNSHIAAQQLMRLGYTDVTVYAGGKQDWEEG
                     GLPFETAEATAAA"
     gene            494..949
                     /locus_tag="VD791_07755"
     CDS             494..949
                     /locus_tag="VD791_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXV07908.1"
                     /translation="MGKSLESTKGSVWALFLTAHAVLVDAIERRLAQADLPPLGWYDL
                     LWALERSPGQRLRMSELAEKVVLSRSNLTRLVDRLEEAGLVSRERSEDDRRGAYAVLT
                     EAGREMRRRMWPVYEGGIRQLFEGRLSEAEAETLGAVLRRLLAAAREIG"
     gene            complement(1125..1394)
                     /locus_tag="VD791_07760"
     CDS             complement(1125..1394)
                     /locus_tag="VD791_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07909.1"
                     /translation="MNAKLISPKLITPKLITLGAAFALLASTSALAGQPFGRDSVYAA
                     PNSPTTKVAAAKPGNGRGSVYASDLPAPTPKDQVRIATTFKPGRA"
     gene            complement(1448..1636)
                     /locus_tag="VD791_07765"
     CDS             complement(1448..1636)
                     /locus_tag="VD791_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07910.1"
                     /translation="MTAKLLTGLLIGALLTSSALADNRIDVSIVNGKQQANFSLGDSK
                     CVLVNEVIRCAPVLVASN"
     gene            complement(1739..2113)
                     /locus_tag="VD791_07770"
     CDS             complement(1739..2113)
                     /locus_tag="VD791_07770"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HXV07911.1"
                     /translation="MKVRRAWLGLGWLGVAGVVVLSLVPAPSVGVEHGDKLGHVATYA
                     LLMWWFAQAWLRRDTRARTAAGLLALGVVLEYAQGWTGFRDFSVADMVADALGIALGW
                     LLAPPRAPNVLQRASAYLTRAG"
     gene            complement(2136..2756)
                     /locus_tag="VD791_07775"
     CDS             complement(2136..2756)
                     /locus_tag="VD791_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXV07912.1"
                     /translation="MLKVWGRNNSVNVQKVLWCCEEMGLKYERIDAGGQYGIVNTPQY
                     RALNPNGLVPTVEDGPFVLWESNTIVRYLSAKHGPGKLWPEDLKVRAEGDKWMDWHNS
                     TFWPAFRPLFWNLVRTPIDQRDEKAMEESRLKTAELLGYLDAHLKHRDYIAGETLTMG
                     DIPMGCAAWRWFGLQFERPELPNLERWFGMLKQRPAYASVVLLPLT"
     gene            complement(2797..3648)
                     /gene="nudC"
                     /locus_tag="VD791_07780"
     CDS             complement(2797..3648)
                     /gene="nudC"
                     /locus_tag="VD791_07780"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HXV07913.1"
                     /translation="MAEVFVPLIAPPQSVSAPGLWFVFQGSQVLVRREEAHAYLPVAL
                     HVREIGLEPLRHQYLGLYAGQHCFSCEVAADVAAPAGMTWAGLRSLFGALDDALFALV
                     GRAIQVMDWDRSHQYCGRCGTPTVVKGAERARQCPSCGQVHYPRIAPAVMALVRNGDA
                     LLLARSPHFPPGMHSALAGFVEPGESLEQCLHREVREEVGLEVTNLRYFSSQPWPFPH
                     SLMIAFNCDYAGGEITPEAGEIEAAAWFGIDDLPVLPNRISIARRLIDAAIAQIRSGR
                     PVTIQPG"
     gene            complement(3658..5454)
                     /gene="lpdA"
                     /locus_tag="VD791_07785"
     CDS             complement(3658..5454)
                     /gene="lpdA"
                     /locus_tag="VD791_07785"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXV07914.1"
                     /translation="MSNLLEVKVPDIGDFKDVPIIEVLVKPGDRVEKETSLITLESDK
                     ATMEVPSPEAGVVKDLRLKLGDKVSQGVVILTLETDGAAAAAPVVKSVGPASKAESPV
                     ARAPAPVAPTAPNTPEPSRPTAGAPERGDYHAEVLVLGAGPGGYTAAFRAADLGKKVV
                     LVERYPTLGGVCLNVGCIPSKALLHAAKVISEAEEMSHLGVQFGTPRIDLDKLRGWKD
                     SVVSRLTKGLAGLARQRKVQVVHGVAKFTAAHMLAVETAEGGKRVSFDHAIIAAGSQA
                     ARIPGFPYDDQRLMDSTSALQLHDTPKRLLVIGGGIIGLEMATVYDALGSKVTVVELL
                     DGLIPGADRDLIRPLHRRIEKRYEAIYLKTKVTRLEPLPEGLRATFEGEGAPAPQVYD
                     RVLLSVGRRPNGKAIDAAAAGVRVDERGYIAVDRQLRTNVPHIFAIGDIVGEPMLAHK
                     ATHEAKVAAEVIAGHKAAFDARTIPSVAYTDPEVAWMGMSETSAKAQGVEVEKAVFPW
                     SASGRALGIARDEGLTKLLFDKSTRRLLGAGIVGPNAGELISETVLALEMGAEAQDIG
                     LTIHPHPTLSETVYFAAEIADGSITDLYLPKR"
     gene            complement(5504..6760)
                     /locus_tag="VD791_07790"
     CDS             complement(5504..6760)
                     /locus_tag="VD791_07790"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HXV07915.1"
                     /translation="MDIQSYMHGVGREARRTSRAMARADTRAKDEALAAMALAIERDA
                     ERLLEANAQDVDAARAKGLEAALIDRLTLTGKSIAAMAQGLREIEQLRDPVGEITDLA
                     YRPSGIQVGRMRVPLGVVAIIYEARPNVTADAAGLCLKAGNAAILRGGSEAIRSNQAI
                     AACVREGLTRAGLPEAAVQVIEVTDRAAVGELITMKDYVDIIVPRGGKGLIERITAES
                     RIPVLKHLDGVCHVYIDERADPDKAIRVADNAKTQRYGTCNTMETLLVHRAIAATVLP
                     PLATIYQAKNVELRGCPRARAIVPLMREAVEADWYTEYLAPILSVRVVDDLDQAMEHI
                     AVYGSQHTDAIVTEDYARARRFLREVDSSSVLVNASTRFADGYEYGLGAEIGISTDKL
                     HARGPVGLEGLTSQKFVVLGDGHVRT"
     gene            complement(6783..7817)
                     /gene="holA"
                     /locus_tag="VD791_07795"
     CDS             complement(6783..7817)
                     /gene="holA"
                     /locus_tag="VD791_07795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HXV07916.1"
                     /translation="MRIDTEQLARHLERTLAPLYTVHGEETLLALEAADRIRQQARAQ
                     GYSEREVLTTEAGFDWSQLAMSANSLSLFASRRILELRIPSGKPGNDGAEALKRIASD
                     LPPDTLVLVSLPRLDRTTLAAGWFQALEQAGVAVAANAVTPARLPQWLGGRLRQQGQE
                     ADADTLALLAGMVEGNLLAAQQELHKLALLFPPGKLTREGVESAVLDVARFDVFKLGE
                     ALLGGDPARLTRVLEGLKGEGAAPPLVLWAIAEELHALWRVSAAVAGGKPLQLALREA
                     RVWGPRAELLPRALRRIRGRELEAALLGAADVDRTIKGLTRGDAWAELLRLALSLTGS
                     QAGNRGRINA"
     gene            complement(7820..8209)
                     /locus_tag="VD791_07800"
     CDS             complement(7820..8209)
                     /locus_tag="VD791_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07917.1"
                     /translation="MLREIAAVRQDRADLRRRWFQDDYFDLFVWVAADGSVAAFQLAY
                     DRSGKERVLGWDRDAGYLHRQVDGGEESVFQKMTPLLTRAGRFPRLEVIAQFDARSPG
                     LEEGIRRFVRDKAARYRSRARRAFRRG"
     gene            complement(8215..>8548)
                     /locus_tag="VD791_07805"
     CDS             complement(8215..>8548)
                     /locus_tag="VD791_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HXV07918.1"
                     /translation="LAIDRTDDAVAHLYDPLHPAVLHLVAQIIRGAHKAGKPVAVCGE
                     MAGDVKLTRILLGFGLRQFSMHPAHLLSIKQQVLRTDLDEVTQFATRMLKTDDPERLH
                     GMLERMNA"
BASE COUNT         1268 a         2945 c         2968 g         1367 t
ORIGIN      
        1 gcggaagcgg ggcgagcgtt gcagccgcgc gggcgcatta cgcggcggcc gtggcctcgg
       61 ccgtctcgaa gggcagtccg ccctcttccc aatcctgctt gcctccggcg tacaccgtca
      121 catcggtgta gccgaggcgc atcagctgct gcgcggcgat atggctgttc tggcacgtgc
      181 ggctggcgca atacacgacg atctgcgcac tgcggtcggg caccacgctc ggcgcgagct
      241 gggcggcctg atcgtgcggg aacagcagcg ccccggggag atgcttctgc gcgtagtagc
      301 gctcgggcag ggcctcgagc aggacggggg gcttgccgga gtcgatacgg cgagccagtt
      361 cttcgcgggc gatggtcctg gtcatgtgta cctccaagga attggttgat gaagatataa
      421 tcgcaactgc aatgcatagg ctagcatgga tgtacttgca attgcaatta ctatttcgtc
      481 tgagagccag ccgatgggaa aatctctcga atcgacaaag ggttccgtct gggccctgtt
      541 tctcaccgcg catgccgtac tggtcgacgc gatcgagcgg cgactggcgc aagcggatct
      601 gccgccactg ggttggtacg atttgctatg ggcgctggag cgttccccgg ggcagcgcct
      661 gcgcatgagc gagttggcgg agaaggtggt cctctcgcgc agcaacctga cgcggctggt
      721 ggaccggctc gaggaagccg ggctggtatc gcgcgagcgc tccgaggacg accggcgcgg
      781 cgcatacgcg gtgctcaccg aggccggccg cgagatgcgg cggcggatgt ggccggtgta
      841 cgaaggcgga atccgccagc tgttcgaagg ccggctgagc gaggccgaag ccgaaacctt
      901 gggcgcggtc ttgcggcggc tgctggcggc cgcgcgcgag atcggttgag aggcactcgg
      961 cgcggacacg agcgagccgg aggtgttcca gcgagtgggt ccccgaaata aagcggcccg
     1021 cgcaaggcgg gccgaagagg gaggagacga ggtcggccgg cggggcggtg cgatgcagac
     1081 gccgcaccgc cccgcgctgc gccccgcccc agggggttcg gggcttacgc gcgcccgggt
     1141 ttgaaggtcg tcgcgatgcg cacctgatcc ttcggcgtgg gcgccggcaa gtcgctggcg
     1201 tacacgctgc cgcgcccgtt gcccggcttg gcggccgcca ccttggtggt agggctgttc
     1261 ggagcggcgt acaccgagtc gcgtccgaag ggctggccgg cgagcgcgct ggtcgaggcc
     1321 agcagggcga aggcggcgcc tagcgtgatc aatttggggg tgatcaattt ggggctgatc
     1381 agtttggcgt tcatctctat ctcctacgtg aaacggttaa caggggaagg gctcgacaga
     1441 caacgaatca gttggaagcc acgagcaccg gagcgcagcg gatcacttcg ttcaccagga
     1501 cgcacttgga atcgcccagg ctgaagttgg cttgctgctt gccgttgacg atgctcacgt
     1561 cgatgcggtt gtcggccagc gcggagctgg tgagcagcgc tccgatcagc agtccggtca
     1621 gcagtttggc ggtcatcgtc gcagtcctcg tccagcgttc gttgttgtga acactgtacg
     1681 gtggacgggg gatgaaaccg caggaactat tgcgaccgga acctgtgaac tatttacctc
     1741 agcccgcccg cgtcaggtag gccgaggcgc gctgcagcac gttcggcgcg cgcggcgggg
     1801 cgagcagcca gcccagcgcg atgcccaggg cgtcggccac catgtcggcg acggagaagt
     1861 cgcggaagcc ggtccagcct tgcgcgtact cgaggaccac acccagcgcc agcaggcccg
     1921 ccgcggtgcg cgcgcgcgtg tcgcgccgca gccaggcctg cgcgaaccac cacatgagca
     1981 gcgcatacgt agcgacgtgc ccgagcttgt cgccgtgctc cacgcctacc gaaggagccg
     2041 ggaccagcga cagcacgacc acaccggcca cgccgagcca gcccagcccg agccaggccc
     2101 tgcgcacctt catgcgcggc ccgcgccgcg gcgcatcagg tcagcggcag cagcaccacg
     2161 ctggcgtagg ccgggcgctg cttgagcatg ccgaaccagc gctccaggtt gggcagctcc
     2221 gggcgctcga actgcaggcc gaaccagcgc caggcggcgc aacccatggg gatgtcgccc
     2281 atggtgagcg tttcgcccgc gatgtaatcg cggtgcttga ggtgcgcgtc gaggtagccg
     2341 agcagctccg cggtcttcag ccgcgactct tccatcgcct tctcgtcgcg ctggtcgatg
     2401 ggcgtgcgca ccaggttcca gaacagcggc cggaaggcgg gccagaacgt gctgttgtgc
     2461 cagtccatcc acttgtcgcc ctcggccctg accttgaggt cctccggcca cagcttgccg
     2521 gggccgtgct tcgcgctcaa atagcgcacg atggtgttgg attcccacag cacgaacggg
     2581 ccgtcctcga cggtggggac caggccgttg ggattgagtg cgcggtattg cggcgtgttg
     2641 acgatcccgt actgcccgcc ggcgtcgatg cgctcgtact tcagccccat ttcctcgcag
     2701 caccacagca ccttctgcac attcacggag ttgttgcgcc cccagacctt gagcatggct
     2761 tccccctgtc gaagtgtcgt gcgtccttgt catgagttat ccgggttgga tggtcaccgg
     2821 ccggcccgag cggatctgcg cgatggccgc gtcgatcagc cgccgcgcga tgctgatgcg
     2881 gttgggcagc accggcaggt cgtcgatgcc gaaccacgcg gccgcttcga tctcgcccgc
     2941 ttcgggcgtg atctcgccgc cggcgtagtc gcagttgaag gcgatcatga gcgaatgcgg
     3001 aaacggccag ggctggctcg agaagtagcg caggttggtc acctcgagcc cgacttcctc
     3061 gcgcacttcc cggtgcaggc actgctcgag gctttccccc ggctcgacga accccgccag
     3121 cgcgctgtgc atgcccggcg gaaagtgcgg cgagcgcgcg agcagcagag cgtcgccgtt
     3181 gcgcaccagc gccatcaccg ccggcgcgat gcgcgggtaa tgcacctgtc cgcagctcgg
     3241 gcactggcgt gcacgctcgg cgcccttgac cacggtaggc gtgccgcaac ggccgcagta
     3301 ctggtgcgaa cggtcccagt ccatcacctg gatggcgcgg ccgaccaagg cgaacagcgc
     3361 atcgtcgagc gcgccgaaca ggctgcgcaa cccggcccag gtcatgcctg cgggcgccgc
     3421 tacgtcggcc gcgacctcgc aggaaaaaca gtgctgcccg gcgtacaggc ccaggtactg
     3481 gtggcgcagt ggttccaggc cgatctcgcg cacgtgcagg gcgaccggca ggtatgcgtg
     3541 cgcctcctcc cgccgcacca gcacctgact gccctgaaac acgaaccaca ggccgggcgc
     3601 gctcaccgat tgcggcgggg cgatgagcgg gacgaacact tcggccatgg ctcgcgctca
     3661 gcgcttcggc aggtacaggt cggtgatgct gccgtccgcg atctccgccg cgaaatacac
     3721 ggtctccgac agcgtcgggt gcgggtggat ggtgagcccg atgtcctgcg cctcggcgcc
     3781 catctccagc gccagcaccg tctccgagat cagctcgccg gcgttcggtc cgacgatgcc
     3841 cgcgcccagc aggcgccgcg tgctcttgtc gaacagcagc ttggtgagcc cctcgtcgcg
     3901 cgcgatgccg agcgcgcggc cgctcgcgga ccaggggaac accgcctttt cgacctcgac
     3961 tccctgcgcc ttggcgctgg tctcgctcat gcccatccac gcgacttccg ggtcggtgta
     4021 ggccaccgaa ggaatggtgc gggcatcgaa cgctgccttg tgcccggcga tcacctcggc
     4081 ggcgaccttg gcttcgtgcg tcgccttgtg cgcgagcatc ggttcgccga cgatgtcgcc
     4141 gatggcgaag atgtgcggca cgttggtgcg cagctgccga tccaccgcga tgtagccgcg
     4201 ctcgtccacc cgcacgccgg cggccgccgc gtcgatcgcc ttgccgttgg gccgccgccc
     4261 caccgacagc agcacgcggt cgtacacctg cggcgcgggc gcgccttcgc cctcgaaggt
     4321 ggcgcgcagc ccctcgggca ggggctcgag acgggtgacc ttggtcttca gatagatcgc
     4381 ctcgtagcgc ttctcgatgc gccggtgcag cggccggatc aaatcgcggt ccgcccccgg
     4441 gatcaatccg tccagcagct ccaccacggt caccttggag ccgagcgcgt cgtacaccgt
     4501 ggccatctcc agcccgatga tgccgccgcc gatcaccagc aggcgcttcg gcgtgtcgtg
     4561 cagctgcagc gcgctggtgg agtccatcag gcgctggtcg tcgtagggga agcccgggat
     4621 acgtgccgcc tgggagccgg cggcgatgat ggcgtggtcg aaggacacgc gcttgccgcc
     4681 ttcggcggtc tccaccgcca gcatgtgcgc cgcggtgaac ttggccacgc cgtgcaccac
     4741 ctgcactttg cgttgcctgg cgagccccgc cagccccttg gtgagacggc tcaccacgct
     4801 gtccttccag ccgcgcagct tgtccagatc gatgcgtggc gtgccgaact gcacgccgag
     4861 gtgcgacatc tcctcggcct cgctgatcac cttggccgcg tgcagcagcg ccttggacgg
     4921 gatgcagccc acgttcaggc acacaccgcc cagggtggga tagcgctcca ccagcaccac
     4981 tttcttgccc agatcggcgg cacggaacgc ggcggtgtag ccgcccgggc ccgcgccgag
     5041 gaccaggact tcggcatgat agtcgccgcg ttcgggcgcg cccgccgtcg ggcgcgaagg
     5101 ctcgggtgtg ttgggcgccg tgggcgcgac gggcgccggt gccctcgcga ccggagattc
     5161 ggccttggat gccggaccta cggacttgac gactggcgca gcggcggcgg cgccgtcagt
     5221 ctccagcgtg aggatcacca caccctggga caccttgtcg ccgagcttga ggcgcaagtc
     5281 cttcaccacg ccggcctcgg gagagggcac ttccatggtc gccttgtccg actccagcgt
     5341 gatcagggaa gtctccttct ccacgcggtc gccgggcttg accagcacct cgatgatggg
     5401 aacgtccttg aaatcgccga tgtcggggac tttcacctcg agcaggttgc tcatggttcc
     5461 aaacccttgt tgtcgtcgcg cgcggcctgg agcggccgcg cacctaggtg cggacgtgac
     5521 cgtcgccgag gacgacgaac ttctgggagg tgagaccttc cagccccacc gggccgcgcg
     5581 cgtgcagctt gtcggtggag atgccgatct ccgcgcccag gccatactcg tagccgtcgg
     5641 cgaagcgcgt ggaggcgttc accagcaccg agctggagtc cacctcgcgc aggaagcgac
     5701 gtgcgcgcgc gtagtcctcg gtgacgatcg cgtcggtgtg ctgcgagccg tagacggcga
     5761 tgtgctccat cgcctggtcc aggtcgtcca ccacgcgcac gctcaggatc ggcgccaggt
     5821 actcggtgta ccagtcggcc tcgaccgcct cccgcatcag cggcacgatc gcacgcgcgc
     5881 gcgggcagcc gcgcagctcc acgttcttgg cctggtagat cgtcgccagg ggcggcagca
     5941 cggtcgcggc gatggcccga tgcaccagca gcgtctccat ggtgttgcag gtgccgtagc
     6001 gctgcgtctt ggcgttgtcc gcgacgcgga tcgccttgtc cggatcggcg cgctcgtcga
     6061 tgtagacgtg gcacacgccg tccagatgct tgagcacggg gatgcgcgac tcggctgtga
     6121 tgcgctcgat caatcccttg cctccgcgcg gcacgatgat gtcgacgtaa tccttcatcg
     6181 tgatcagctc gcccaccgcg gcgcggtcgg tcacctcgat cacctgcacc gcggcctccg
     6241 gcagtccggc gcgagtaagg ccctcgcgca cgcaggcggc gatcgcctgg ttggagcgga
     6301 tcgcctcgga gccgccgcgc aggatggcgg cgttgccggc cttcaggcac agcccggcgg
     6361 cgtcggcggt gacgttgggg cgcgcctcgt agatgatcgc caccacgccc agcggcacgc
     6421 gcatgcggcc gacctggatg ccgctggggc ggtaggcgag atcggtgatc tcgcccaccg
     6481 ggtcgcgcag ctgctcgatc tcgcgcaggc cctgcgccat cgcggcgatg ctcttgccgg
     6541 tgagagtgag gcgatcgatc agcgccgctt ccaagccctt ggcgcgggcg gcgtccacgt
     6601 cctgcgcgtt ggcttcgagc aggcgctcag catcgcgctc gatggcgagc gccatggcgg
     6661 cgagcgcttc gtccttggcg cgggtgtcgg cccgcgccat cgcgcgggag gtccggcgcg
     6721 cctcgcgccc cacgccgtgc atgtaagact ggatgtccat tcgcggggac cgcggcagca
     6781 gatcaggcgt ttattctacc gcgattgccc gcctgcgatc ctgtcaggct gagagcgagg
     6841 cgcagcagct ccgcccatgc gtcgccgcgc gtcaagccct tgatcgtgcg atccacgtcg
     6901 gccgcgccca gcagcgccgc ctccagctcg cgcccgcgga tgcgccgcag cgcgcgcggc
     6961 agcagctccg cgcgcgggcc ccacacgcgc gcctcgcgca gagcgagctg cagcggcttg
     7021 ccgccggcca ccgcggccga tacccgccac agcgcgtgca gctcctcggc gatcgcccac
     7081 agcaccagcg gcggcgcggc accttcgccc ttcagccctt ccagcacgcg cgtgaggcgc
     7141 gccgggtcgc cgccgagcag cgcctcgccc agcttgaaca cgtcgaagcg cgccacgtcg
     7201 agcacggcgg actcgacccc ttcgcgcgtc agcttgcccg gggggaacag cagcgccagc
     7261 ttgtgcagct cctgctgggc ggcgagcagg ttgccttcga ccatgcccgc gagcagcgcc
     7321 agcgtgtcgg catcggcctc ctgcccctgc tgccgcaggc gtccgcccag ccactgcggc
     7381 aaccgcgcag gcgtgacggc gttggcggcg accgccacgc cggcttgttc cagcgcctgg
     7441 aaccaaccgg cggccagcgt ggtgcgatcc agcctgggca acgacaccag caccagcgtg
     7501 tccggcggca gatcggaggc gatgcgcttg agcgcttcgg cgccgtcgtt ccccggcttg
     7561 cccgacggga tgcgcagctc caggatgcgg cgcgaggcga acagcgacag gctgttggcg
     7621 ctcatcgcga gctgcgacca gtcgaatccc gcttcggtgg tgagcacctc gcgctccgag
     7681 tagccttgtg cccgcgcctg ctgccggatg cgatcggccg cctccagcgc gagcagggtc
     7741 tcttcgccat gcaccgtgta cagcggcgcc agagtgcgct cgaggtgccg ggcgagctgt
     7801 tcggtgtcga tgcgcacggt caaccgcggc ggaacgcacg ccgcgcgcgt gaacgatagc
     7861 gtgccgcttt gtcgcgcacg aagcggcgga tgccttcctc caggccgggg ctgcgggcgt
     7921 cgaactgcgc gatcacctcg agcctgggga agcgccccgc gcgcgtgagc agcggggtca
     7981 tcttctggaa caccgactct tcgccgccgt cgacctggcg gtgcagatag ccggcgtcac
     8041 ggtcccagcc cagcacgcgt tccttgccgc tgcggtcata ggcgagctgg aacgcagcga
     8101 cgctgccgtc cgcggcgacc cacacgaaca ggtcgaaata gtcgtcctgg aaccagcgcc
     8161 ggcgcaggtc cgcccggtcc tgcctcaccg ccgcaatctc gcgtagcatc ccggtcaagc
     8221 gttcatccgc tcgagcatgc cgtggagccg ttcgggatcg tcggtcttga gcatgcgggt
     8281 ggcgaactgc gtgacctcgt cgagatcggt gcgcagcacc tgctgcttga tgctgagcag
     8341 atgggccgga tgcatggaga actggcgcag cccgaagccc agcagaatgc gggtgagctt
     8401 cacgtccccg gccatttcgc cgcacaccgc gaccggcttg ccggccttgt gcgcgccgcg
     8461 gatgatctgc gccaccaggt gcagcaccgc ggggtgcagc gggtcgtaca ggtgcgccac
     8521 cgcgtcgtcg gtgcggtcga tggcgagc
//
LOCUS       DASPGE010000241         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_648902, whole genome shotgun sequence.
ACCESSION   DASPGE010000241 DASPGE010000000
VERSION     DASPGE010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648902"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VD791_07810"
     CDS             complement(<1..659)
                     /locus_tag="VD791_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07919.1"
                     /translation="MRRILATCLAALAAAQAAVYADDTDIFLTSSGTGGIAPNVLIVL
                     DNSANWSANFDGGTKFTAEMSTLAGVIGTLDDRINLGLMMMDETGAGTTAPTSGSYVR
                     YAVRNMEPTNRMALRNMVTNMGQNADKTNNASWGFAMFEAFKYFGGGSGSPQSPTQFG
                     RDAYSGFGQPKRDFPGNIVTNTAGGLPGHAFASQVSRTYVSPISDSCQKSYVIFIGNG
                     MP"
     gene            complement(730..1212)
                     /locus_tag="VD791_07815"
     CDS             complement(730..1212)
                     /locus_tag="VD791_07815"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HXV07920.1"
                     /translation="MVLTLHPMRSPHRQRGAVLFVALIMLVVFTLIAVSGVSSSIAGL
                     RVVGNVQSQTEVSAAAQLALEQVVDEVGNFQYPTDTPPPTQSVTVSIAGLSYPVTVTL
                     HCLGSAPVAGYSASFAASAPSESYWDIRASASDPRSGGKTVVHQGVRVTLSPGQVCPT
                     "
     gene            complement(1215..2285)
                     /locus_tag="VD791_07820"
     CDS             complement(1215..2285)
                     /locus_tag="VD791_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HXV07921.1"
                     /translation="MNAQRFAPAGQAGISMVEMMVAVTLGLFILAGIGLTLSSTSHSR
                     AELDRALQQIENSRYAMQMLAQDLRHAGYYGRYAAELAVPAAMPDPCAATLAALEVGM
                     PLPVQGYDAPATVPPELAPCLSSANLVPGTDILVVRRAQTGQPIDVAAASVGQVYLQT
                     TAYPSGPRYVLGSGSNPAAFTLIEKRDDHSGATVPAGLLPYRVHVYFVSPCDIPTTGD
                     ACNGADDDGGRPIPTLKRLELAADVGGNPVMRTVPLVEGIQDLQVDYGIDQPAPGASA
                     GDGSPDTYTTAPATPADWGNVVAVQLNLLAVNTEPTAGHADGKRYNLGLAGIVGPFGD
                     AYKRHALSAVVRVNNVSGPREQ"
     gene            complement(2282..2869)
                     /gene="pilV"
                     /locus_tag="VD791_07825"
     CDS             complement(2282..2869)
                     /gene="pilV"
                     /locus_tag="VD791_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HXV07922.1"
                     /translation="MLMNRTASMPRQRGFAIIEVLVTLVIVLLGMLGAAGVLVRSHQS
                     SMEAYQRTQALVLVEDMLDRMSANRPVADCYAVTDPGAGAPHLGTGYTGVPDCGLGTP
                     GQQATAIRDLQQWSQLLQGAAETDAGGAFVGAMIGARGCVSYDVATDTYSVTVTWQGL
                     GPTSAPPDEVRCAVGLYGDDAQRRALTLTMRIADL"
BASE COUNT          405 a          961 c         1042 g          465 t
ORIGIN      
        1 ggcatgccgt tgccgatgaa gatcacatag ctcttctggc agctgtcgct gatcgggctc
       61 acgtaggtgc ggctgacctg cgaggcgaag gcgtggccgg gcagcccgcc ggccgtgttg
      121 gtgacgatgt tgccggggaa atcgcgcttg ggctggccga agcccgaata ggcgtcgcgg
      181 ccgaactggg tgggactctg cggcgagccg ctgccgccac cgaagtactt gaacgcctcg
      241 aacatggcga agccccacga ggcgttgttg gtcttgtccg cgttctgccc catgttggtg
      301 accatgttgc gcagcgccat gcggttggtc ggctccatgt tacgcaccgc atagcgcacg
      361 tagctgccgc tggtgggcgc ggtggtgccg gcgccggttt cgtccatcat catcagccca
      421 aggttgattc ggtcgtcgag cgtgccgatc acgcccgcga gcgtgctcat ctcggcagtg
      481 aacttggtgc cgccgtcgaa gttggcgctc cagttcgcgc tgttgtccag cacgatgagc
      541 acgttgggcg cgatgccgcc cgtgccggac gacgtcagga agatgtcggt gtcgtcggcg
      601 tagaccgccg cctgtgccgc ggccaatgcc gccaggcagg ttgcgaggat tcgtctcatg
      661 tgcgcgctcc gcgaacgtgc gggcgggcgg cccgcgtggc tcagtccatc gattcggtcc
      721 gtccggcgat caggtggggc acacctggcc cgggctcagg gtgactctca cgccctggtg
      781 cacgacggtt ttgccgccgc tgcgggggtc gctggcgctg gcgcggatgt cccagtagga
      841 ctcgctcggc gccgaggccg cgaacgatgc gctgtatccg gccaccggcg ccgagcccag
      901 acagtgcaag gtgacggtca ccggatagct caacccggcg atcgagacgg tcacgctctg
      961 cgtcggcggc ggggtgtcgg tggggtactg gaagttgccc acctcgtcga ccacctgctc
     1021 gagagcgagc tgggcggcgg cgctgacctc ggtctgcgac tgcacgttgc ccaccacgcg
     1081 cagcccggcg atgctcgagc tcacgcccga cacggcgatc agcgtgaaca ccaccagcat
     1141 gatcagcgcg acgaacagca ccgcgccgcg ctgcctgtgc ggcgagcgca tgggatggag
     1201 ggtaaggacc atggtcattg ctcccgggga ccgctgacgt tattgacgcg caccaccgcg
     1261 gacagcgcgt ggcgcttgta ggcgtcgccg aacggcccga cgattcccgc caggccgagg
     1321 ttgtagcgct tgccgtcggc gtgcccggcg gtgggctcgg tgttgacggc gagcaggttg
     1381 agctgcaccg cgaccacgtt gccccagtcg gcgggggtcg ccggcgcagt ggtgtaggtg
     1441 tcaggcgagc cgtcgccggc gctggcgccg ggcgccggct ggtcgatgcc gtagtcgacc
     1501 tgcagatcct ggatgccctc gaccagtggc acggtgcgca tcaccgggtt gccgcccacg
     1561 tcggcggcca gctccaggcg cttcaaagtg gggatcgggc gcccgccgtc gtcgtcggcg
     1621 ccgttgcagg cgtcgcctgt ggtcggaatg tcgcaggggc tgacgaagta cacgtgcacg
     1681 cggtagggca gcagcccggc cggcaccgtc gcgcccgagt ggtcgtcgcg cttctcgatc
     1741 agggtgaagg cggctggatt cgagccgctg cccagcacgt agcgcggtcc gctggggtaa
     1801 gccgtggtct gcagatagac ttgtcccacc gaggcggcgg cgacgtcgat cggctggccg
     1861 gtctgggccc ggcgcaccac caggatgtcg gtgcccggca ccaggttggc gctcgacagg
     1921 cagggcgcga gctcgggcgg caccgtcgcg ggtgcgtcgt agccttgaac cggcagcggc
     1981 atgccgacct ccagcgcggc gagcgtcgcc gcgcaggggt ccggcatcgc cgccgggacg
     2041 gccagctcgg ctgcgtagcg gccgtaatag ccggcgtggc gcagatcctg cgccagcatc
     2101 tgcatcgcgt agcggctgtt ctcgatctgc tgcaatgcgc gatcgagctc cgcgcgcgaa
     2161 tggctggtgc tgctcagcgt caggccgatc ccggcgagga tgaacaggcc cagggtcacg
     2221 gccaccatca tctcgaccat ggagatgcct gcctgccctg cgggcgcaaa gcgctgcgcg
     2281 ttcataggtc ggcgatccgc atggtgagag tcagcgcgcg ccgctgcgca tcgtcgccgt
     2341 acagcccgac ggcgcagcgg acctcgtcag gcggcgcaga cgtcggcccc aatccctgcc
     2401 aggtcaccgt cacggagtag gtgtcggtgg ccacgtcgta gctcacgcag ccgcgcgccc
     2461 cgatcatcgc gcccacgaaa gcgccgccgg cgtcggtttc ggcggcgccc tgcagcagct
     2521 ggctccattg ctgcaggtcc cggatcgcgg tggcttgctg ccctggcgtc cccaagccgc
     2581 agtccggcac gccggtgtag ccggtgccca ggtgcggcgc gcccgcgccc ggatcggtga
     2641 cggcatagca gtcggcgacc ggacggttcg cgctcatgcg gtcgagcatg tcttccacca
     2701 ggaccagcgc ctgcgtgcgc tggtaggctt ccatcgaaga ctggtggctg cgcaccagca
     2761 cgccggcggc gcccagcatt cccagcagga cgatgaccag cgtcaccagc acctcgatga
     2821 tggcgaagcc gcgctgccgc ggcatggagg cggttcggtt catcagcacg ctc
//
LOCUS       DASPGE010000242         3266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1350712, whole genome shotgun sequence.
ACCESSION   DASPGE010000242 DASPGE010000000
VERSION     DASPGE010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350712"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="VD791_07830"
     CDS             complement(<1..1227)
                     /locus_tag="VD791_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXV07923.1"
                     /translation="MIEGLVALAAMLVLCFIRIPIAFSMAITGVVGYAYMRDWNFTVA
                     FAVVQTNVYETGRNYTLSVVPLFLLMGTFVARAGIAEQLFRAAYAFIGHLRGGLAMAT
                     VVACAGFGSICGSSIATAATMAKVAYPSMKRFGYSDALSAGAIAAGGTLGIMVPPSTL
                     MVIYGVFTETNIGKMFAAGILPGILGALLLCGAVQWACWRDPKAGPPGERASWKVRLA
                     SLAGIWPVALLFLFVIGGIYGGLFTATEGAGMGAFAAMAFALWRRALTWKTLYQSLVD
                     SGRTTAMLFMILIGALIFADFINITSMPADLRAFVTRFELHPVLVVAAIMLIYILLGT
                     AMEELSMVLLTLPVFFPLIVGLGLDPVWFGVLVVTIVEIGLISPPVGMNLFVLSTLLP
                     SVRTTTVFRGVLPFMAA"
     gene            complement(1224..1727)
                     /locus_tag="VD791_07835"
     CDS             complement(1224..1727)
                     /locus_tag="VD791_07835"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXV07924.1"
                     /translation="MTGVPGVGPAQQEHRWERRADAALGIAASAILFAMMTLTFVDVI
                     LRYVFNRPLRGGFEVTELLLLVLIFAGLPLVTHANEHVTMDLIDRWLGARARNALSRV
                     MEAVCAALMFALTWFMWLKAQRISGYGDTTDVLRIAVGPFVYFMVAMILLSGLIHLYR
                     ALAPGSR"
     gene            complement(1724..2746)
                     /locus_tag="VD791_07840"
     CDS             complement(1724..2746)
                     /locus_tag="VD791_07840"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXV07925.1"
                     /translation="MITKQELALAALVAGAFAAQPAAAQTTLTFSSWVPPTHHLTLWQ
                     QAWAAQAEKATGGRVKFHSLPKAPAAPPGTFDAVRDGLVDLSYVTASYTPARHVFPLM
                     PELPGAGNTAEINSVAYSRIHWKHFHKFGEYNGVKLLAVWTHGPGQMFTKKPVKGIGD
                     IQGLKIRTGGGVAEAVAKALGASAFVKPAPESYELLKSGVADGVFFPLESVVSFKLDT
                     VLEQATIFPGGMYSSAFGFFMNQDKWAKLSKQDQAIIEKMSGEAAARSNGKSWDTADQ
                     KGLAALKTAKVKIVQADKALLAEVRKRSDPIIEDWIAKANAKGADAPAILAEFRAELK
                     KVAAGK"
     gene            2818..>3266
                     /locus_tag="VD791_07845"
     CDS             2818..>3266
                     /locus_tag="VD791_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HXV07926.1"
                     /translation="MAASGWVDLRSDTVTRPSAGMRRAMAEAEVGDDVFGDDPTVNRL
                     QERAAELFGFEASLFFPTGTQSNLAALISHCQRGDEVLVGMEAHTYRYEAGGGAVLGS
                     IQPQAIVNRPDGTMDLKDVEAAIKPDDPHFAKTRLLALENTITGRVIP"
BASE COUNT          557 a         1093 c         1163 g          453 t
ORIGIN      
        1 ggccgccatg aacggcagca cgccgcggaa caccgtggtg gtgcgcaccg acggcagcag
       61 cgtcgacagc acgaacaggt tcatgcccac cggcgggctg atgaggccga tctcgacgat
      121 ggtcaccacc agcacgccga accacaccgg gtcgagcccg aggcccacga tcagcggaaa
      181 gaacaccggc agcgtgagca gcaccatgga gagctcctcc atcgcggtgc cgaggaggat
      241 gtagatcagc atgatcgccg ccaccaccag caccggatgc agctcgaagc gcgtcacgaa
      301 ggcgcgcagg tcggccggca tgctggtgat gttgatgaag tcggcgaaga tcagcgcgcc
      361 gatcaggatc atgaacagca tggcggtggt gcgcccgctg tccaccagcg actggtacag
      421 ggtcttccag gtgagcgcgc ggcgccacag ggcgaaggcc atggcggcga aggcgcccat
      481 gccggcgccc tcggtggcgg taaacaggcc cccgtagatg ccgccgatca cgaacaggaa
      541 gagcagggcc accggccaga tgccggccag cgaggccaag cgcaccttcc aggaggcgcg
      601 ttccccgggc gggccggcct tcgggtcgcg ccagcaggcc cattgcaccg ccccgcacag
      661 cagcagcgcc ccgaggatgc cgggcaggat gccggcggcg aacatcttgc cgatgttggt
      721 ctcggtgaac acgccgtaga tcaccatcag cgtggagggc gggaccatga tgcccagcgt
      781 ccccccggcg gcgatggcgc cggccgagag cgcgtccgaa tagccgaagc gcttcatcga
      841 ggggtaggcg accttggcca tggtggcggc ggtggcgatc gaggagccgc agatcgagcc
      901 gaagcccgcg caggcgacca ccgtcgccat ggcgaggccg ccgcgcaggt ggccgatgaa
      961 ggcgtaggcg gcgcggaaca gctgctcggc gatgccggcg cgcgcgacga aagtccccat
     1021 cagcaggaac agcggcacca ccgacagcgt gtagttgcgc ccggtctcgt agacgttggt
     1081 ctgcaccacg gcgaaggcca cggtgaagtt ccagtcgcgc atgtaggcgt agccgaccac
     1141 gcccgtgatc gccatcgaga aggcgatggg gatgcggatg aagcacagca cgagcatcgc
     1201 cgcgagggcg accaggccct cgatcatcga ctgcccgggg cgagcgcgcg gtacaggtgg
     1261 atcaggccgg agagcaggat catcgccacc atgaagtaga cgaacggccc caccgcgatg
     1321 cgcagcacgt cggtggtgtc gccgtagccc gagatgcgct gcgccttcag ccacatgaac
     1381 caggtgagcg cgaacatcag cgccgcgcac accgcctcca tcacccggct caacgcgttg
     1441 cgcgcgcgcg cccccagcca gcggtcgatc aggtccatgg tgacgtgctc gttggcgtgc
     1501 gtcaccagcg gcaggccggc gaagatcagc accagcagca ggagctcggt gacctcgaag
     1561 ccgccgcgca gcggccggtt gaagacgtag cgcaggatga cgtccacgaa ggtgagcgtc
     1621 atcatggcga acaggatcgc ggaggccgcg atgccgagcg ccgcgtcggc gcggcgctcc
     1681 cagcgatgtt cctgctgcgc ggggccgacc ccgggtaccc cggtcacttg ccggcggcga
     1741 ccttcttcag ctcggcgcgg aactcggcga ggatggcggg cgcgtcggcg cccttggcgt
     1801 tggccttggc gatccagtcc tcgatgatcg ggtcggagcg cttgcgcact tcggccagca
     1861 gcgccttgtc ggcctgcacg atcttcacct tcgccgtctt cagcgccgcg aggcccttct
     1921 ggtcggccgt gtcccaggac ttgccgttgc tgcgcgcggc ggcctcgccg gacatcttct
     1981 cgatgatcgc ctggtcctgc ttcgagagct tcgcccactt gtcctggttc atgaagaagc
     2041 cgaacgccga gctgtacatg ccgcccggga agatcgtcgc ctgctcgagc acggtgtcca
     2101 gcttgaacga cacgaccgac tccagcggga agaacacgcc gtccgccacg cccgacttga
     2161 gcagttcgta ggactcgggc gcgggcttga cgaacgccga ggcgccgagc gccttggcca
     2221 ccgcctcggc gacgccgccg ccggtgcgga tcttcaggcc ctggatgtcg ccgatgccct
     2281 tgaccggctt cttggtgaac atctggccgg ggccgtgcgt ccacacggcg agcagcttca
     2341 cgccgttgta ctcgccgaac ttgtggaaat gcttccagtg gatgcgcgag taggcgaccg
     2401 agttgatctc cgcggtgttg ccggcgccgg gcagctcggg catcagcggg aacacgtggc
     2461 gcgcgggcgt gtagctcgcg gtgacgtagg acaggtcgac gaggccgtcg cgcaccgcgt
     2521 cgaaggttcc gggcggcgcg gcgggcgcct tgggcaacga gtggaacttg acgcgcccgc
     2581 cggtggcctt ctcggcctgc gcggcccagg cctgctgcca gagcgtgagg tggtgggtcg
     2641 gcggcaccca cgacgaaaag gtgagcgtcg tctgcgcggc cgcgggctgc gcggcgaaag
     2701 cgccggcgac gagcgcggca agggcgagtt cttgtttggt tatcatggtc tcctccggaa
     2761 agtggcgggg gcctcctgcc cctcgtgcgc cggagtgtag cgcaacaggg ggagccaatg
     2821 gcagcgagcg gctgggtgga cctgcgcagc gacacggtga cgcggccctc ggccggcatg
     2881 cgccgggcga tggccgaggc cgaggttggc gacgacgtgt tcggcgacga ccccaccgtc
     2941 aacaggctgc aggaacgcgc ggcggagctt ttcggcttcg aggcctcgct cttcttcccc
     3001 accggcacgc aatccaacct cgccgcgctg atctcccact gccagcgcgg cgacgaggtg
     3061 ctggtcggca tggaggcgca cacctaccgc tacgaagcgg gcggtggggc ggtgctcggc
     3121 tcgatccagc cgcaggcgat cgtcaaccgt cccgacggca cgatggacct gaaggacgtc
     3181 gaggccgcga tcaagccgga cgacccgcac ttcgcgaaga cgcggctcct tgcgctggag
     3241 aacaccatca ccgggcgagt gatccc
//
LOCUS       DASPGE010000243         7710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1118505, whole genome shotgun sequence.
ACCESSION   DASPGE010000243 DASPGE010000000
VERSION     DASPGE010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7710
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118505"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VD791_07850"
     CDS             <1..876
                     /locus_tag="VD791_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXV07927.1"
                     /translation="VKVNRIEAGDDQLLERIRNEGTNSPADVLLIVDAARLTNAERLG
                     LFAPLKSAILEQRIPPEVRHPQGLWFGFSSRARVIVYDAKSVDPRDIQTYEDLAAPSN
                     KGKVCTRPGSHPYMLSLVSAMIEHLGEPRAEAWARGVVANFARPPRGGDTDQIRAVAA
                     GECGIALTNSYYLVRLMRSAKPEDRAVAQAVGLVWPDQKGHGTHMNVSGGGLMRNAPH
                     RANAIRFLEYLASDAAQGYFANGNNEWPVVRGAAAGNPALAALGDFRRDPLSIAALGR
                     NVAAAQRIVDRVGWR"
     gene            complement(873..2549)
                     /locus_tag="VD791_07855"
     CDS             complement(873..2549)
                     /locus_tag="VD791_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXV07928.1"
                     /translation="MASAVFLERFRRDRAGIGWLGYGALAVALLVTLPLAGLAFGVLA
                     PGTPGVWSHLAQTVLPRYVANSVLLALAVALGVALLGVACAWATAGFEFPGRRWLEWA
                     LVLPLAMPAYVVAYAYTDFLQFSGPVQSALRALTGWRAREYWFPEIRSLPGAAVLFVL
                     VLFPYVYLPVRAAFLERSPNLLDAARLSGLGAAAAFRRAVLPLARPALAAGIALAVME
                     TLADYGAVSYFALETLTIGIYKAWFGLGERTAAAQLSLLLLAAVALIMWAERVSRGRA
                     RFQGSGTLAAGRTRLAGANGWAVALLCFLPVLLGFLLPAAILLRLAARDLELVHFGRY
                     AESMVNSFAAAGLAALVATGLALLLAYAARLRPATAVRAGVRLAALGYAVPGAVLAVA
                     ILVPLARLDNALDAFLQAHFGWRSGLLLTGSVAALVYAYVVRFLALALSNAEAGLARI
                     TPSMDEAARSLGCGPARLAVWVHAPLLARSLLIGMLLVFVDGLKELPATLVLRPFDFE
                     TLATQTYNLAKDERLGEAALPSLTIVAVGLIPMVLVSSALGRRRFERRAR"
     gene            2660..3748
                     /locus_tag="VD791_07860"
     CDS             2660..3748
                     /locus_tag="VD791_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV07929.1"
                     /translation="MLPSPGPLVELRGVAHAYAEHRIIDSLSFAVPRGSIACLLGPSG
                     CGKTTLLRCIAGFEPLAAGEILLDGELVSRPGFMQPPERRRIGMVFQDYALFPHLSVA
                     ANVAFGLSRQPRAEREQRMREMLRAVGLADSAHRYPHALSGGQQQRVALARALASRPR
                     LLLLDEPFSSLDVELRERLAGEVRALLKAQDTTAILVTHDQQEAFALADEIGVMRDGR
                     ILQWDNAYNLYHRPADRFVADFVGLGNFVPGRVTRGGQVEVELGALQGELHFAGEALP
                     DDGGAPVSMPVDVLLRPDDVVHDDSSPVRARVVGKAFRGAEILYTLRLSSGAQVLSLV
                     PSHHDHALGEAIGVRLAADHVIVFPRAP"
     gene            complement(3781..4338)
                     /locus_tag="VD791_07865"
     CDS             complement(3781..4338)
                     /locus_tag="VD791_07865"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HXV07930.1"
                     /translation="METSTAGARYSNVAIALHWLIAVGVLVMIGLGWTMTDIPKGTPE
                     RAYFFNLHKSIGVTVGVLALIRLWWRATHRPPALPGSMASWEVTAAKVNHALLYACII
                     AMPVIGFLASNFTKFGVKYFGLQIGPFFPENQALRDGLQQVHGLLSYLLVVLVVVHVL
                     AAFKHLLVDKDGVFQRMLPGAHGRV"
     gene            complement(4579..4650)
                     /gene="pqqA"
                     /locus_tag="VD791_07870"
     CDS             complement(4579..4650)
                     /gene="pqqA"
                     /locus_tag="VD791_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HXV07931.1"
                     /translation="MWTKPEFTDMRFGFEVTMYIYNR"
     gene            complement(4810..5529)
                     /locus_tag="VD791_07875"
     CDS             complement(4810..5529)
                     /locus_tag="VD791_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXV07932.1"
                     /translation="MADRRVGRSPNDMLNETQVASGTAAMAERAIRVMLVDDHAVVRM
                     GFRLLLQGTADIKVTGEADSGEEAVRMYAEARPHVVVMDISMPGIGGLEAIGRILARE
                     PMARILVLSGHEDVMHARRVLKAGALGYLTKRSAPEALIQAIRQVHQGKTYLEPSIAQ
                     QLAMQQLGGEKSPVDMLSEKEFKVFLALARGKSVAEIAQTMSLSPRTVGTHLYNIKQK
                     LGASNSAELAIIAIRAGLIEP"
     gene            complement(5617..7347)
                     /locus_tag="VD791_07880"
     CDS             complement(5617..7347)
                     /locus_tag="VD791_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXV07933.1"
                     /translation="MSLRVRINLIITTLIVLFTLVIGNIIVEEMRSAIREEMEAGTKI
                     TLQHLNTVLYGSDLAHSDGAEARVLLRFLQHLGRVRAHEIRMYDARGRLVYTSPPSAY
                     KAGRSAPEWFARLMWPESGEFTLDTAVARVVVAPDASRSILDAWDDLVRLSWLLLGFL
                     VVVNAAVFFLLGRSLRPVRAILAGMSEMQRGKFDVRLPSFPLPEFDSIGRTFNGMAQA
                     LEESHAEARRLALVARQSSDATVIHDLQGNISFWNPAAARLFGYRSEEIVGRSATLLT
                     PPGLESEVADNLRVICARRVIENLETQRLARDGRVVDVALSAAPLVDPASEQVIGEIV
                     TLRDITEHKRAQQAEADLKQNRRLTRAIQTHLEEERRSIARELHDELGQCVTAIRTIG
                     TAIANRARPNAPEIHDSARTIVEVAGHIYDVVHGIIRRLRPSALDNLGLTEALEEWLA
                     SWRERHPDIGVRLDLSGDLDNLGETVNITVYRIVQECLTNVVRHAAASRVEVAIARSG
                     GCLEVSVRDNGKGLGERNESESARFGLMGMRERVEGLAGEFQIDSRPGEGLRVRASIP
                     VPAAAPVLAE"
     gene            complement(7344..>7710)
                     /locus_tag="VD791_07885"
     CDS             complement(7344..>7710)
                     /locus_tag="VD791_07885"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXV07934.1"
                     /translation="YESALMAVGEAPGAPSPAADDDAVLDRVRVHLHYLANDCFLQPG
                     QLLSALAALAEIPAILVQGRRDLVCPPATAYTVKQAWRSARLCMVEDGGHSAAHPAMA
                     AALVRATEDMKTLLGQTPE"
BASE COUNT         1218 a         2739 c         2660 g         1093 t
ORIGIN      
        1 gtcaaagtca accgcatcga ggcaggcgac gatcaactgc tcgagcgcat ccgcaacgag
       61 ggcaccaaca gccccgcgga cgtgctgctg atcgtggacg ccgcgcgcct gaccaatgcc
      121 gagcggctgg ggctgttcgc gccgctgaag tccgcgatcc tggagcagcg catcccgccg
      181 gaggtgcgcc atccccaggg cctgtggttc ggcttttcca gccgcgcgcg cgtgatcgtg
      241 tacgacgcca agtcggtgga cccgcgcgat atccagacct acgaggacct cgccgcgccg
      301 tccaacaagg gcaaggtgtg cacgcggccg ggcagccacc cctacatgct gtcgctggtg
      361 agcgcgatga tcgagcacct cggcgagccg cgggccgagg cctgggcgcg cggtgtggtg
      421 gccaacttcg cgcgcccgcc gcgcggcggc gacaccgacc agatccgcgc cgtggccgcg
      481 ggcgagtgcg gcatcgcgct caccaacagc tactatctgg tgcgcctgat gcgatcggcc
      541 aagccggagg atcgcgcggt ggcgcaggcc gtcggcctgg tgtggcccga tcagaagggc
      601 cacggcacgc acatgaacgt ctcgggcggc ggcctgatgc gcaacgcccc gcaccgcgcc
      661 aacgcgatcc gcttcctcga atacctggcg agcgacgcgg cgcaggggta cttcgccaac
      721 ggcaacaacg aatggccggt ggtgcgcggc gccgcggccg gcaacccggc gctcgccgcc
      781 ctcggcgatt tccggcgcga tccgctcagc atcgccgcgc tgggccgcaa tgtcgccgcc
      841 gcgcagcgca tcgtcgatcg cgtggggtgg cgctagcgcg cccgtcgctc gaacctgcgc
      901 cggcccagcg cactggacac cagcaccatc gggatcaggc cgaccgccac gatggtgagg
      961 ctgggcagcg cggcctcgcc caggcgctcg tccttggcca ggttgtaggt ctgggtggcg
     1021 agcgtctcga aatcgaacgg gcgcaacacg agcgtggccg gcagctcctt gagcccgtcg
     1081 acgaacacca gcagcatgcc gatcaacagg ctgcgcgcca gcagcggcgc gtgtacccac
     1141 accgccagcc gcgccgggcc gcagccgagg ctgcgggcgg cttcgtccat gctcggcgtg
     1201 atgcgcgcaa gccccgcttc cgcgttggac agcgccagcg ccaggaagcg caccacgtag
     1261 gcgtacacca gggccgccac gctgccggtg agcagcaacc cgctgcgcca tccgaagtgc
     1321 gcctgcagga aggcgtccag cgcgttgtcc aggcgcgcca gcggcaccag gatggcgacc
     1381 gcgagcaccg ccccgggcac cgcgtagccc agcgcggcca ggcgcacgcc ggcgcgtacc
     1441 gccgtcgccg gacgcaggcg cgcggcgtaa gcgagcagca gggccaggcc ggtggcgacg
     1501 agcgcggcca ggcccgcggc ggcaaagctg ttcaccatgc tctcggcata gcggccgaag
     1561 tgcaccagct cgaggtcgcg cgcggccagg cgcagcagga tcgcggccgg caacaggaat
     1621 ccgagcagta ccggcaggaa gcacagcagc gcgaccgccc agccgttcgc gccggccagg
     1681 cgcgtgcgac cggccgccag cgtgccactg ccctggaagc gggcgcgccc gcgcgagacg
     1741 cgctcggccc acatgatcag cgccacggcg gcgagcagca gcagcgacaa ctgggcggcg
     1801 gcggtgcgct cgcccaggcc gaaccaggcc ttgtagatgc cgatggtgag cgtctccagc
     1861 gcgaagtacg acaccgcgcc atagtcggcc agcgtctcca tcacggcgag cgcgatgccc
     1921 gccgcgagcg cggggcgggc gagcggcagc accgcgcgcc tgaaggccgc cgccgcgccg
     1981 agcccgctca ggcgcgcagc gtcgagcaga ttcggggaac gctccaggaa ggcggcgcgc
     2041 accggcaggt acacgtaggg gaacaggacc agcacgaaca gcaccgccgc gcccggcagc
     2101 gagcggatct ccgggaacca gtactcgcgc gcccgccagc cggtgagcgc gcgcagcgcg
     2161 ctctgcaccg gcccgctgaa ctgcaggaag tcggtgtagg cgtacgccac cacgtaggcg
     2221 ggcatggcca gcggcagcac cagcgcccat tccagccagc gccgccccgg gaactcgaac
     2281 ccggcggtgg cccaggcgca ggccacaccc agcagcgcga cgccgagcgc cacggcgagc
     2341 gcgagcagta cgctgttggc cacatagcgc ggaagcaccg tctgcgccaa gtgcgaccag
     2401 acgccggggg tgccgggcgc cagcacaccg aacgccagtc cggccagcgg cagtgtcacc
     2461 agcagcgcca ccgccagtgc gccgtagccg agccagccga tgccggcgcg atcgcgccgg
     2521 aaacgctcga gaaaaaccgc agaagccaag ggaaaccggg gcacggtggg tcgcgcgcgt
     2581 cgtcgccccg agggccatcg tacgcaggcg cgccattcta cgcgttccac gcccgggggc
     2641 cgctatactc acgcgcgtca tgctcccctc acccggcccg ctggtcgagc tgcgcggcgt
     2701 ggcccacgcc tacgcggaac accggatcat cgactcgctg tctttcgcgg tcccgcgcgg
     2761 gagcatcgcc tgtctgctcg ggcccagcgg ctgcggcaag accacgctgc tgcgctgcat
     2821 cgccgggttc gaaccgctgg ccgccggcga gatcctgctc gacggcgagc tggtcagccg
     2881 ccccggcttc atgcagccgc cggagcggcg ccgcatcgga atggtgttcc aggactacgc
     2941 cctgttccct cacctcagcg tcgcggccaa cgtcgccttc gggctgtcac ggcaaccgcg
     3001 cgcggagcgc gagcagcgca tgcgcgagat gctgcgcgcg gtggggctcg cggacagtgc
     3061 gcacaggtac ccgcacgccc tgtccggcgg gcagcagcaa cgcgtggcgc tggcccgggc
     3121 gctggcgtcg cgcccgcgcc tgctgttgct ggacgagccc ttctccagcc tggacgtgga
     3181 gctgcgcgag cgcctggccg gcgaggtccg cgccctgctc aaggcgcagg acacgacggc
     3241 gatcctggta acgcatgatc agcaggaggc ctttgcgctc gccgacgaga tcggcgtcat
     3301 gcgcgacggc cgcatcctgc agtgggacaa cgcgtacaac ctgtatcacc gtcccgccga
     3361 ccgtttcgtc gccgacttcg tggggctcgg aaacttcgtt ccgggccgcg tgacgcgcgg
     3421 cggccaggtg gaggtcgagc tgggggcgtt gcagggcgag ttgcacttcg caggcgaagc
     3481 gcttccggac gacggcggcg cgccggtgtc gatgccggtg gacgtgctgc tgcggccgga
     3541 cgatgtggtg cacgacgaca gcagcccggt gcgcgcccgg gtggtgggca aagcgttccg
     3601 cggcgcggag atcctctaca ccctgcggct gtcgagcggc gcccaggtgc tgtcgctggt
     3661 gcccagccac cacgaccacg ccctcggtga agcgatcggc gtgcggctgg cggccgatca
     3721 cgtgatcgtg ttccccaggg caccgtgagc gcctgcgggc cctcgcggtg gccgcgcggt
     3781 tcagacccgg ccgtgtgcgc ccggcagcat acgctggaac actccgtcct tgtccaccag
     3841 caggtgcttg aacgccgcga gcacgtgcac caccaccagc accaccagca ggtacgagag
     3901 caggccgtgg acctgctgta agccgtcgcg caacgcctgg ttctcgggga agaacggacc
     3961 gatctgcagg ccgaagtact tgaccccgaa cttggtgaag ttcgaggcga ggaagccgat
     4021 gaccggcatg gcgatgatgc atgcgtacag cagcgcgtgg ttgaccttcg ccgcggtcac
     4081 ctcccagctc gccatcgagc cgggcagcgc aggcgggcga tgcgtcgccc gccaccacag
     4141 gcggatcagc gccagcacgc cgaccgtgac gccgatcgac ttgtgcaggt tgaagaagta
     4201 ggcgcgctcg ggcgtgccct tggggatatc ggtcatggtc cagcccaggc cgatcatgac
     4261 caggacaccc acggcgatca gccagtgcag cgcgatcgcc acgttgctgt agcgcgcgcc
     4321 ggcggttgac gtctccatgc aagccccctc ccggttggtg gccgctatgc tactggagag
     4381 cccccgcaaa aggatagcgg gcccaggcgg ggcaccgctt cgggcccgcg atccggatcc
     4441 cggcccgtga tcgcagcccc ttgtgaggat tgatctttct tcgagtgtgg gcggcccttt
     4501 atccacgccc tggggggagg gaaatgagga ccaaaaaaaa gggctgccgg taggcagccc
     4561 ttatggtgag caactcgttc agcggttgta gatgtacatc gtcacttcga agccgaagcg
     4621 catgtcggtg aattcaggct tggtccacat ggtgtagctc ctttgcgaag acttctcgtt
     4681 ggtaacagac gatggggcga ctcaacccac gggaagcatt ttgcagatcg ggcgggcacc
     4741 cggccagcgt gaatcccctg attcgcgcct catgattttc attaggcggg cccacccgga
     4801 agggggccat cagggctcga tcagcccggc acggatggcg atgatggcca gttcggccga
     4861 gttggaggcg ccgagcttct gcttgatgtt gtacaggtgg gtgcccacgg tgcgcgggct
     4921 gagcgacatg gtctgcgcga tctctgccac cgacttcccc cgggcgagcg ccaggaacac
     4981 cttgaactcc ttctcgctca acatgtccac cgggctcttc tcgccgccca gctgctgcat
     5041 ggcgagctgc tgggcgatgg agggctcgag gtaggtcttg ccctgatgca cctgacggat
     5101 cgcctggatc agcgcctccg gggcgctgcg cttggtgaga tagcccagcg cgccggcctt
     5161 gagcacgcgg cgggcgtgca tcacgtcctc gtgccccgac agcaccagga tccgcgccat
     5221 cggctcccgc gccaggatgc ggccgatggc ctccagcccg ccgatgcccg gcatggagat
     5281 gtccatcacc accacgtggg ggcgcgcctc cgcgtacatg cgcaccgcct cctcgccgct
     5341 gtcggcctcg cccgtcacct tgatgtcggc ggtcccctgc agcagcagcc ggaagcccat
     5401 gcgcaccacg gcatggtcgt ccaccagcat cactcgaatg gcgcgctctg ccatcgccgc
     5461 cgtcccggaa gcaacctgcg tttcgttcag catgtcgttc ggcgagcgcc cgacacgtcg
     5521 gtcggccaac gcctctctca ttagaccagc accgctctca ttcggtcgac gccgctttca
     5581 ttctgccaac acgccatcat tcggccaaca ccgctctcat tcggccaata cgggggccgc
     5641 ggccggcaca gggatcgagg cccgcacgcg cagaccctcg ccggggcggc tgtcgatctg
     5701 gaattcgccg gccagcccct cgacgcgctc gcgcatgccc atcagcccga agcgcgccga
     5761 ttcggattcg ttgcgctctc ccaggccctt gccgttgtcg cgcaccgaca cctcgaggca
     5821 gccgccgctg cgggcgatgg cgacctccac gcggctggcc gccgcgtgac ggaccacgtt
     5881 ggtgaggcac tcctgcacga tgcgatacac ggtgatgttg acggtttcgc cgagattgtc
     5941 cagatcgccg gaaagatcga gccgtacgcc gatgtcggga tggcgctcgc gccagctggc
     6001 cagccattcc tccagcgcct cggtgaggcc caggttgtcg agcgcactgg ggcgcagcct
     6061 gcgtatgatg ccatgaacca catcgtagat gtggccggcc acctcgacga tggtgcgcgc
     6121 gctgtcgtgg atctccggtg cgttgggtcg ggcgcggttg gcgatggccg tgccgatggt
     6181 gcggatggcg gtgacgcact gccccagctc gtcgtgcagc tcgcgcgcga tcgagcggcg
     6241 ctcttcctcc aggtgcgtct ggatcgcccg cgtcaggcgc cggttctgct tgaggtcggc
     6301 ctccgcctgc tgcgcgcgct tgtgctcggt gatatcgcgc agggtgacga tctcgccgat
     6361 gacctgctcg ctcgccgggt ccaccagcgg cgcggcggac aaggccacgt ccaccacccg
     6421 cccgtcgcgg gcgagccgct gggtctcgag gttctcgatc acgcgccgcg cgcagatgac
     6481 gcgcaggttg tccgccactt cggactccag cccgggaggc gtgagcaacg tcgcgctgcg
     6541 gcccacgatc tcctccgaac ggtaaccgaa caagcgcgcg gccgccgggt tccagaacga
     6601 gatgttgccc tgcaggtcgt ggatcaccgt ggcgtcgctc gactgccggg cgaccagcgc
     6661 cagccggcgg gcctcggcat ggctttcctc gagcgcctgc gccatgccgt tgaaggtgcg
     6721 gccgatggag tcgaactccg gcagcgggaa cgagggcagg cgcacatcga acttgccgcg
     6781 ctgcatctcg gacatgccgg cgaggatggc gcgcaccggc cgcagcgacc gtcccagcag
     6841 gaagaacacc gccgcgttga ccaccaccag gaacccgagc agcagccagc tcagtcgcac
     6901 caggtcgtcc caagcatcca ggatcgagcg cgaggcgtcc ggcgcgacca ccacgcgcgc
     6961 aaccgccgtg tcgagggtga actcgcccga ttccggccac atcaggcgtg cgaaccactc
     7021 gggggcggag cgcccggcct tgtacgcgga cggcggggac gtatagacca gcctgccgcg
     7081 ggcgtcgtac atgcggatct cgtgcgcgcg cacgcggccc agatgctgga ggaagcgcag
     7141 cagcactctg gcctcggcgc cgtcgctgtg cgccagatcg ctgccgtaca ggacggtgtt
     7201 gagatgctgc agcgtgatct tggtgccggc ctccatctcc tcgcggatgg cgctgcgcat
     7261 ctcctccacg atgatgttgc cgatcaccag cgtgaacagc acgatcagcg tggtgatgat
     7321 cagattgatg cggacgcgca ggctcattcc ggagtctgtc ccagcagcgt tttcatgtct
     7381 tcggtggcgc gcaccagcgc cgccgccatg gccggatgcg ccgccgaatg gccgccgtct
     7441 tccaccatgc acagccgcgc cgagcgccat gcctgcttga cagtgtacgc ggtcgccggg
     7501 gggcagacca ggtcgcgccg tccctgcacc aggatcgccg ggatctcggc aagcgcggcg
     7561 agcgcgctca gcagctgccc gggttgcagg aagcaatcgt tcgcaaggta gtgcagatgc
     7621 acgcgcacgc ggtcgagcac cgcgtcgtcg tctgcggcgg gcgatggcgc gcccggggct
     7681 tcgcccaccg ccatcagcgc gctctcgtac
//
LOCUS       DASPGE010000244         3588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1235426, whole genome shotgun sequence.
ACCESSION   DASPGE010000244 DASPGE010000000
VERSION     DASPGE010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235426"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1140)
                     /locus_tag="VD791_07890"
     CDS             complement(205..1140)
                     /locus_tag="VD791_07890"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXV07935.1"
                     /translation="MLLLLGAAWCRAQDLPAQTIRVVVPLSAGSSLDARARVIADAIG
                     QRIKRRVIVENRPGAGGTIGTQYVARSKPDGATLLFTNNSHVVGPHIYGNPGYDPIKD
                     LAPVARAYVSGMVLVAHPGLRVGSIEELVALARASTAAPSYASSGTGGLPHLAMAMFA
                     RSANIELLHVPYRGDAQGLTDVLAGRVSVMISGYPAALSHVRAGSLRALAVTSSQRAD
                     IFPGVRTMAEAGYPGAVLDAWTGFFAPAGTPRTVIDRLNREIAAALALPKLQAHFAAT
                     GAQASAGSPDEFGALVKQEWERYGKLVRELGLRVE"
     gene            1470..>3588
                     /locus_tag="VD791_07895"
     CDS             1470..>3588
                     /locus_tag="VD791_07895"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXV07936.1"
                     /translation="MQPVSAISRSVSRRPGLFLKYAVPLVILVSGALIVSGLLEIFFS
                     YRENKEALSRIQHEKAVSASIRIEQFVRELERQLAWIAQTPWGPRGVPLDQRRLDSLR
                     LLRHVPAVTEVSHLDPTGHEQLRVSRLAMDVVGSNVDMSQDAKFVEARDRKTYFSPVY
                     FRKESEPYMTIALAGMGEDTGVVAAEANLKFIWDVVSSMKIGEAGAAYVVDSAGRLIA
                     HPDISLVLQKTDLSHLPQVSGAAAALANPDAATIDALIGRNLQGREVLAGYAAIAPLG
                     WIVFVDLPLREALAPLYQSLLRTVVLLAVGVLISVVASLWLVRRMVAPVHVLQTGAAK
                     IGAGALEHRIEVKTGDELEALAEEFNRMTGRLQESYVGLERKVEERTRDLSEALEQQT
                     ATAEILRVISSSPTDLQPVLDAVAASAAQLCGATDAVIFRIKDDRYWVAAAWGSMPKL
                     RTDEMLPIRRDFLGGRAILEGRIVHIPDLHLADDDFAGAKEYGARLHYRAALAAPMLR
                     EGVPIGVIFIVRPEAGPFSEKQIGLLKTFADQAVIAIENVRLFKELEARTSDLGRSVE
                     QLKALSEVGQAVNSTLDLQEVLNTIVARAVQLSAADTGAVFEHDETSTTLELRATYGI
                     QPELVDELMVRTLRMGEGATGRAAATRAPVEIADVQAVDVYTGKMRDVLDRAGYRAML
                     AVPLVQGERVLGGLTVTRRTPGKF"
BASE COUNT          580 a         1174 c         1253 g          581 t
ORIGIN      
        1 cgaggcgggt gcgggccagg tgctgctgtc gcagcgcgtg gtggcctcgg tcgaggacat
       61 ggccgaagtg gaaccggtgg gcgcgctgcc gctgaagggc ttcctcaagc cggtgccggt
      121 gtaccggctg gtggggctga aaggctgacg gcgccgcgct cgggcgcccc cgacctcgct
      181 accgggcgat cagacgagcg ggcttcactc gactctcagg cccagctcgc gcaccagctt
      241 accgtagcgc tcccactcct gcttcaccag cgccccgaac tcgtccggag agccggcgga
      301 ggcttgcgcg ccggtcgcgg cgaaatgcgc ctgcagcttg ggcagggcca gagcggccgc
      361 gatctcgcgg ttcagccggt cgatcactgt gcggggcgtg cccgcgggcg cgaagaagcc
      421 ggtccaggcg tccaggacgg cgcccgggta gccggcttcg gccatggtcc ggactccggg
      481 gaatatgtcc gcgcgctgcg agctggtcac cgccagcgcg cgcaagctgc ctgccctgac
      541 gtgcgacagc gcggccgggt agccgctgat catcaccgac acccgtcccg cgagcacgtc
      601 ggtcagcccc tgcgcgtcgc cccggtaggg cacgtgcagc agctcgatgt ttgcgctgcg
      661 cgcgaacatc gccatggcca gatgcggcag gccgccggtg ccgctggacg cgtagctcgg
      721 cgctgcggtg ctggctctcg ccagcgccac cagttcctcg atggacccaa cgcgcaggcc
      781 gggatgggcc accaggacca tcccgctgac ataggcgcgc gcaaccggtg ccagatcctt
      841 gatcgggtcg tagcccgggt tgccgtagat atgcgggccg acgacgtggc tgttgttggt
      901 gaacagcagc gtcgcgccgt ccggcttcga cctggccacg tattgggtgc cgatggtgcc
      961 gcccgcgccg ggccggttct cgacgatgac ccggcgcttg atccgctgcc cgatggcatc
     1021 cgcgatgacg cgagcgcgcg catccaggct gctgccggcg ctgagcggca ccaccacgcg
     1081 gatggtctgc gccggcaggt cctgcgcacg gcaccacgcc gccccgagca gcagcagcaa
     1141 agcagcagca caaaccggca ccctccgcat cgctcccctc cgttggggtc ggacctgcct
     1201 aacgcaatga tccgacaagg taacagcgcc gacaaccgcc gatttggacg gtttagagct
     1261 gcgtttctat ggccagaaac cgtgctgtaa aacaccttgg tcgaatcggt gaagatcgac
     1321 gacaccggca ctgcgggcaa tcctgtgcgc gcttggcagc ccgattgtgg cgccgaaatg
     1381 ccgctctaag gcctccaaag ggcctgtgaa cgacgaatga tcctgggatt acggacagtt
     1441 agcgcggtcc gacccctgct gatacactca tgcagcccgt gtccgctatt agccgcagcg
     1501 tgagccgccg tcccggcttg tttctcaagt acgccgtgcc gctcgtcatc ctggtgagcg
     1561 gcgcgctgat cgtcagcggg ctgctcgaga tcttcttctc ctaccgcgag aacaaggaag
     1621 cgctgtcgcg catccagcac gagaaggcgg tcagcgcctc gatccgcatc gagcaattcg
     1681 tgcgcgagct ggaacgccag ctcgcctgga tcgcgcagac gccctggggg ccgcgcggcg
     1741 tgccgctcga tcagcgccgc ctcgattcgc tgcgcctgct gcggcacgtg cccgcggtca
     1801 ccgaggtcag ccacctcgac ccgaccgggc acgagcagtt gcgcgtctcg cgtctggcca
     1861 tggacgtggt gggcagcaac gtggatatgt cgcaggacgc caagttcgtc gaggcgcgcg
     1921 accgcaagac ctacttcagc ccggtgtact tccgcaagga gtccgagccg tacatgacca
     1981 tcgccctggc aggcatgggc gaggacaccg gcgtggtcgc cgcagaggca aacctgaagt
     2041 tcatctggga cgtcgtgtcc agcatgaaga tcggcgaagc cggcgcggcc tacgtggtgg
     2101 acagcgccgg gcgcctgatc gctcaccccg acatcagcct ggtgttgcag aagaccgacc
     2161 tctcgcactt gccgcaggtc agcggtgcgg cggcggcgct ggcgaacccc gatgcggcca
     2221 ctatcgacgc gttgatcggt cgcaatctgc agggacgcga ggtgctggcc ggatacgccg
     2281 cgatcgcgcc tctgggatgg atcgtgttcg tcgacctgcc gctgcgcgag gcgctcgcac
     2341 cgttgtacca gtccttgctg cgcaccgtcg tgctgctggc ggtcggcgta ttgatctcgg
     2401 tggtggcgag cctgtggctg gtgcggcgca tggtggcgcc cgtgcatgtg ctccaaaccg
     2461 gggccgccaa gatcggcgcc ggggcgctgg agcatcgcat cgaggtgaag accggggacg
     2521 agctggaagc gctggcggaa gagttcaacc gcatgaccgg ccgcctgcag gagtcctacg
     2581 tcggcctaga gcgcaaggtg gaggagcgca cgcgcgatct gagcgaggcc ttggagcagc
     2641 agacggcgac cgcagagatc ttgcgcgtga tcagcagctc gcccaccgat cttcagcccg
     2701 tgctggatgc ggtcgccgcc agtgcggcgc agttgtgcgg tgcgaccgat gcggtgatct
     2761 tccgcatcaa ggacgacaga tactgggtgg cggcggcttg gggatcaatg cccaagttgc
     2821 ggaccgatga aatgctgccg attcgccgag acttcctggg cggccgcgcg attctcgagg
     2881 gcaggatcgt gcatattcca gatctccacc tggcggatga cgacttcgca ggtgcaaagg
     2941 aatatggcgc gcggctgcac tatcgtgcgg cacttgctgc accgatgctt cgcgaaggcg
     3001 tgccgatcgg ggtgatcttc atcgtgcggc ccgaagcagg acctttctcg gagaagcaaa
     3061 tcggtctgct gaagaccttc gccgaccagg ccgtcatcgc catcgagaac gtccgcctgt
     3121 tcaaggagct ggaggctcgc acgtcggatc ttggccgctc ggtcgagcag ctcaaggctc
     3181 tgtccgaggt gggtcaggcg gtgaactcga cgctggactt gcaggaagtg ctgaacacca
     3241 tcgtcgcgcg ggcggtccag ctgagtgcgg ccgacaccgg agcggtgttc gagcacgatg
     3301 aaacgagcac cacgctggag ctgcgggcga cttacgggat ccagcccgag ctggtggacg
     3361 agttgatggt gcgcacgctg cgcatggggg agggcgccac cgggcgtgcc gccgccaccc
     3421 gcgcgccggt cgagatcgcc gacgtccagg cggtagatgt gtacacgggc aagatgcgcg
     3481 acgtgctcga ccgtgccggc taccgagcta tgctggcggt tccgctggtg cagggagagc
     3541 gcgtgctcgg gggcctcacg gtcacgcgtc gcaccccggg caagttcc
//
LOCUS       DASPGE010000245         4407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_650035, whole genome shotgun sequence.
ACCESSION   DASPGE010000245 DASPGE010000000
VERSION     DASPGE010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650035"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VD791_07900"
     CDS             complement(<1..651)
                     /locus_tag="VD791_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV07937.1"
                     /translation="MSRNRTLLIGLAALVLLPLLVRALGLGVNTATQIVTLAIAALGL
                     NLLVGFTGLTSFGHSAWFGIGAYAAAISQKHWFAGQIGLPIAFSIAFVALLSFLVGLL
                     ILRRRGVYFALLTLAFAALTYTIAFRWTELTGGEDGLGGLARGGFQAVPLDNHNVYYA
                     FVALVAAGVLYGLLRVTRSPFGHVLVAIRENQQRATFQGYHVDRYRLGVFVLSAVVT"
     gene            complement(648..1523)
                     /locus_tag="VD791_07905"
     CDS             complement(648..1523)
                     /locus_tag="VD791_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV07938.1"
                     /translation="MQLLFLLEQVVNGLVLGGYYLLIALGLSLIFSVGGVVNLAHGAF
                     YALGAYGAVEIAKHLGFGPAVALSPVAVALLGILFEKFLLRRFYTADPILSLLVTFGL
                     ALVAEQAIRMIWGAAPLPFSMPQAFKGNVVLGDFLFSRYRLFLLAVVALVLAGIWFLI
                     HRTSFGRVVRAGIQRPDMVAALGIRLQPYMTAIVMLGVGMAALGGALFAPITNVHPAM
                     GAEIITVAFVVVVIGGLGSFWGVVLAALLVGVVRGITIHFVPAAGEASMYILMFLLLM
                     FRPRGLLGERIEKFE"
     gene            complement(1574..2800)
                     /locus_tag="VD791_07910"
     CDS             complement(1574..2800)
                     /locus_tag="VD791_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV07939.1"
                     /translation="MTNRRDLLKAGTAGALALGFPAIVRAQSDAIKIGHLTPMTGFLG
                     ALGVYAVKGIQIAAEEINAAGGVLGRKLDVMSEDSVNPATASTKAQRMLDQDKVDVLM
                     GEINSASAKTIMQVAARSKRLFLQVGARSDVLRGKDCNRYTFHVDIPVTVMVNAAGQA
                     LAREGYIKGKKIFFLTSDYLFGHDLAKAAKAFCAANGGTVVGDELTATDTTDFSPFLL
                     KIRQAKPDLVATNLGGNQVTNFVKQYSEFGLPYQVAGFNLNTADAWSAGEGNLAGIWP
                     TVWHHDLDIPGSKAFVAKFRQKHGQPPENHAWIEYVSLKIMAQAINATKGTDTEKLIA
                     YFESQAEFDLLKSRKGYFRSWDHQLIQEAYPFTVKPKGQAKDKWDFLKFGAAVPAPSE
                     PLESINPPKSANACVL"
     gene            complement(2797..3204)
                     /locus_tag="VD791_07915"
     CDS             complement(2797..3204)
                     /locus_tag="VD791_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV07940.1"
                     /translation="MSTPRKRRDTAFVEQMARVAWHEYPGHFGGALSKALVGPLNSGS
                     QLMDFRISRYAPNAYVQEHSHKVQEQVYYVLEGEGVLTVGKERHLMRPHDYVYLPPGV
                     PHSFTNTGTAGLVFLVITTPADDEPPTKGGKGK"
     gene            3345..4004
                     /locus_tag="VD791_07920"
     CDS             3345..4004
                     /locus_tag="VD791_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07941.1"
                     /translation="MPLQPQEGMLAIWHDVLAGQEAAVSHWYNTEHHAERLSVEGFLS
                     AHRYRLAAGEGRQFLSLYRTRTPAVLSSAAYRARLESPSPRTREIMPTYRNMSRTVCR
                     LALQQGIPEGGHLATLAGTGSPPDPARVFAELLQQPGTLRCRWLLPEDPTLVAQSTAE
                     GTLRGGDASVAWAALVDTNEMQEAVAILAALEAALRPAQVAQRAAYRLVYAGRRETGI
                     S"
     gene            4040..>4407
                     /locus_tag="VD791_07925"
     CDS             4040..>4407
                     /locus_tag="VD791_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXV07942.1"
                     /translation="MDLRQLRYFAQIVESGSLSKASRQLFIAQPALSQQVSKLEDEVG
                     RPLLNRSSRGVAPTENGLALYHHARFMLRQLDQALSIARKESGAVQGMVSVGLPATTV
                     SALGLPLVRRVRERYPSILL"
BASE COUNT          741 a         1480 c         1526 g          660 t
ORIGIN      
        1 cgtcactacc gccgaaagga cgaacacgcc gaggcggtag cggtccacgt ggtagccctg
       61 gaaagtcgcg cgctgctggt tctcgcggat cgccaccagc acgtggccga acggcgagcg
      121 cgtgacgcgc agcaggccgt agagcacgcc ggcggcgacg agcgcgacga aggcgtagta
      181 gacgttgtgg ttgtcgagcg gcaccgcctg gaagccgccg cgcgcgagcc ccccgagccc
      241 gtcctcgccg ccggtgagct cggtccagcg gaaggcgatc gtgtaggtga gcgcggcgaa
      301 ggcgagcgtg agcagcgcga aatacacgcc gcgccggcgc aggatcagca ggccgacgag
      361 gaacgagagc agcgcgacaa aggcgatcga gaaagcgatc ggcaggccga tctgcccggc
      421 gaaccagtgc ttctgcgaga tcgccgcggc gtaggcgccg atgccgaacc aggcgctgtg
      481 gccgaacgac gtgagcccgg tgaagcccac cagcaggttg aggcccagcg cggcgatggc
      541 gagcgtgacg atctgcgtgg cggtgttcac gcccaggccg agcgcgcgca cgagcagcgg
      601 cagcagcacc agcgccgcga ggccgatgag cagcgtgcgg ttgcggctca ttcgaacttc
      661 tcgatgcgct cgcccaggag gccacgcggt cggaacatca gcaggaggaa catcaggatg
      721 tacatcgacg cctcgcccgc cgccggcacg aagtggatgg tgatgccgcg caccacgccc
      781 accagcagcg cggcgagcac cacgccccag aagctgccca ggcccccgat caccaccacc
      841 acgaaggcca ccgtgatgat ctcggcgccc atcgccgggt gcacgttggt gatcggcgcg
      901 aacagggccc cgcccagcgc cgccatgccg acgcccagca tcacgatggc ggtcatgtag
      961 ggctgcagcc ggatgccgag cgcggccacc atgtcgggcc gctggatgcc tgcgcgcacc
     1021 acgcgcccga aggaggtgcg gtggatcagg aaccagatgc cggcgagcac cagcgccacc
     1081 acggcgagca ggaacaggcg atagcgcgag aacaggaaat cgcccagcac gacattgccc
     1141 ttgaacgcct gcggcatgga gaagggcagc ggcgccgcgc cccagatcat gcggatcgcc
     1201 tgctcggcga cgagcgcgag gccgaaggtg acgagcagcg acaggatcgg gtccgcggta
     1261 tagaagcggc gcaacaggaa tttctcgaaa aggatgccga ggagggccac ggcgacgggc
     1321 gacagcgcca ccgcggggcc gaagcccagg tgcttggcga tctcgaccgc gccgtaggcg
     1381 ccgagcgcgt agaacgcgcc gtgggcgagg ttcaccaccc cgccgacgct gaagatgagc
     1441 gacaggccga gcgcgatcag caggtaatac ccgccgagga ccaggccgtt gacgacctgc
     1501 tcgagaagaa agagcagttg catgaatcag ccgcgccgcg acgccgtgac gggcgccgcg
     1561 gcgcaggacg cgcctacagc acgcaggcgt tcgccgactt gggcgggttg atgctctcca
     1621 gcggctcgct cggcgccggc acggcagcgc cgaacttgag gaagtcccac ttgtccttcg
     1681 cctggccctt gggcttgacg gtgaacgggt aggcttcctg gatcagctgg tggtcccagg
     1741 agcggaagta gcccttcctc gacttgagca ggtcgaactc cgcctggctc tcgaagtagg
     1801 cgatcagctt ctcggtgtcg gtgcccttgg tggcgttgat cgcctgcgcc atgatcttga
     1861 gcgacacgta ctcgatccag gcgtggttct ccggcggctg gccgtgcttc tggcggaact
     1921 tggcgacgaa ggccttcgag cccgggatgt cgagatcgtg gtgccagacg gtgggccaga
     1981 tgccggccag gttgccctcg ccggccgacc aggcgtccgc ggtgttcagg ttgaagcccg
     2041 cgacctggta gggcaggccg aactcggagt actgcttgac gaagttcgtc acctggttgc
     2101 cgcccaggtt ggtcgccacg aggtccggct tcgcctggcg gatcttcagc aggaacgggc
     2161 tgaagtcggt ggtgtcggtc gccgtgagct cgtcgcccac gaccgtgccg ccgttcgcgg
     2221 cgcagaaagc cttcgctgcc ttcgccaggt cgtggccgaa caggtagtcc gaggtgagga
     2281 agaagatctt cttgcccttg atgtagccct cgcgcgccag cgcctgcccc gcggcgttca
     2341 ccatcaccgt cacgggaatg tcgacgtgga aggtgtagcg gttgcagtcc ttgccgcgca
     2401 gcacgtcgga gcgcgcgccg acctgcagaa agaggcgctt gctgcgcgcc gccacctgca
     2461 tgatggtctt ggcggaagcc gagttgatct cgcccatcag cacgtccacc ttgtcctggt
     2521 cgagcatgcg ctgcgccttg gtcgaggcgg tggccgggtt gaccgagtcc tccgacatga
     2581 cgtcgagctt gcggcccagc accccgcctg cggcgttgat ctcctccgcg gcgatctgga
     2641 tgcccttcac cgcgtagacg ccgagcgcgc cgaggaaccc ggtcatcggc gtgaggtggc
     2701 cgatcttgat ggcgtcggac tgcgcgcgca cgatcgcggg aaagccgagc gcgagcgcgc
     2761 ccgcggtgcc tgccttcagt aggtctctcc ggttggtcat tttcccttcc ctcccttggt
     2821 cggtggttca tcgtccgccg gcgtcgtgat cacgaggaac acgagcccgg cggtaccggt
     2881 gttggtgaag ctgtgcggca cgcccggcgg caggtacacg tagtcgtgcg gccgcatgag
     2941 gtgccgctcc ttgcccacgg tcagcacgcc ctcgccctcc agcacgtagt agacctgctc
     3001 ctgcaccttg tgcgaatgct cctgcacgta ggcgttgggg gcgtagcgcg agatgcggaa
     3061 atccatcagc tgcgagccgg agttcagcgg gccgacaagc gccttggaca gcgcgccgcc
     3121 gaaatggccc ggatactcgt gccaggcgac gcgcgccatc tgctctacga aggcggtgtc
     3181 gcggcgcttg cgtggggtgc tcatgggtgt gctccggagt ctaggaactg ggtggcagga
     3241 ggaggaaata ccgtttaccg aggctcgata taacgccagc ttatagccaa ccccggaaaa
     3301 cgctattttc ccgcttggcg tggggtccct atgatcgccc gcacatgccg ctgcaacctc
     3361 aagaaggaat gctcgccatc tggcacgacg tgcttgccgg ccaggaagcg gccgtgtccc
     3421 actggtacaa caccgagcac cacgccgagc gcctgtccgt cgagggcttt ctctcggcgc
     3481 accgctaccg gctcgccgcc ggagaaggca ggcaattcct cagtttgtac cgcacgcgaa
     3541 cgccggccgt cctgtcgtcc gccgcttacc gcgcgcgcct ggagtcgccc tcgccgcgca
     3601 cgcgcgagat catgccgacc taccggaaca tgtcgcgcac ggtgtgccgc cttgccctgc
     3661 aacaagggat accggagggc ggccatctcg caacgctggc cggcacgggt tctcctccgg
     3721 atcccgccag ggtcttcgcg gaacttctgc agcaacccgg cacgctgcgc tgccgctggc
     3781 tgttgcccga ggacccgacc ctggtcgcgc aatccacggc ggaaggcacc ctgcgcggcg
     3841 gcgacgcgag cgtcgcctgg gcggcgctcg tcgacaccaa cgagatgcag gaggcagtgg
     3901 cgatcctcgc ggccctcgaa gccgccctgc gccccgcgca agtggcgcag cgcgcggcct
     3961 accggctggt gtacgcggga aggcgcgaaa ccggtataag ctgagcctat atccggaacg
     4021 ccttgggagg aggaccccgg tggacttgcg ccagcttcgc tatttcgccc agatcgtcga
     4081 gagcggcagc ctgtccaagg cctcgcgcca gctgttcatc gctcagcccg cgctcagcca
     4141 gcaggtgagc aagctggagg acgaggtcgg ccggccgctg ctcaaccgct cctcgcgcgg
     4201 cgtcgcgccg accgagaacg gccttgcgct ctaccaccac gcgcgcttca tgctgcgcca
     4261 gctcgaccag gcgctgtcga tcgcgcgcaa ggagtccggt gcggtccagg gcatggtctc
     4321 ggtgggcctg ccggcgacca cggtctcggc gctggggctg ccgctggtgc gccgggtccg
     4381 cgagcgctac ccgagcatcc tgctcaa
//
LOCUS       DASPGE010000246         7920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1235769, whole genome shotgun sequence.
ACCESSION   DASPGE010000246 DASPGE010000000
VERSION     DASPGE010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7920
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235769"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VD791_07930"
     CDS             complement(<1..191)
                     /locus_tag="VD791_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07943.1"
                     /translation="MTALLASAPWLHLVEDPAPSASPAAPAVGPASDGARSAGDDADL
                     LDAYSRAVVQVADAASPAVV"
     gene            complement(274..2007)
                     /locus_tag="VD791_07935"
     CDS             complement(274..2007)
                     /locus_tag="VD791_07935"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HXV07944.1"
                     /translation="MRRALCALLLLLAGAAGAGERILSFDSRVEIQPEGGLLVTETIR
                     VRAEGDRIRRGIFREFPTLYTDRRGNRTTVSFAPVSVRRDGATEPYHTARRSNGVALY
                     IGDKDRVLSPGEYTYEIRYRTDRQLGFFPEHDELYWNVTGVDWDFPIDAASAEVLLPA
                     GASAESVRLEGYTGPMGAKWQNYRAQPRDGVARFETTRPLATREGLTIVVMWPKGLVS
                     APTASARLRHFLRDNRPLTFGALGLLCVLGYYLMMWRRVGRDPAGDVVIPRYQPPEGE
                     TPASMRYLQRMAYDNRCFVAGILGLAVKGYLTIEQEGGGFLKKGKYTLQRTRGSQTPL
                     GLDEHALLREVLGARDTLLLDDENHAILRQARSAHDAALKRKYLKSFFHVNGKWHALG
                     IFLTGLAVALGVVAPAIAGGYGQDWFLLTPGGWATIALGAVALIANAVFGKLLKAPTR
                     EGRRRLDEIEGFRLYLDVAEGDELKLAGAPRKTPGLFEAYLPFALALGVEQRWSEKFA
                     GVFLTQSHSPDWYHGDRWDPNDLGGFSSSLSSSFDSAVSSASTAPGDSSGGGGGGSSG
                     GGGGGGGGGGW"
     gene            complement(2007..2552)
                     /locus_tag="VD791_07940"
     CDS             complement(2007..2552)
                     /locus_tag="VD791_07940"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXV07945.1"
                     /translation="MTVIVAVIAAALIVWGIVAYNLLVRDRARVAAAWSDVDVQLKRR
                     HDLIPKLVEVVQQHAGYERGVLERLVLTRSQAQEASGPAGKSRPEAALGRDLHDLMAV
                     AEAYPELKASQGFLDLQRGLSQAEDQIQYARRYYNGTVNNLNTRVDTFPDLLVARLLG
                     FRAAEYFELQAPGEAQPAEVR"
     gene            complement(2650..3189)
                     /locus_tag="VD791_07945"
     CDS             complement(2650..3189)
                     /locus_tag="VD791_07945"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HXV07946.1"
                     /translation="MFSRLNSRSCSQLAGLAALCVLLAGGAAAADLQVEVRNVKPRKG
                     EVRAALFDSAEAFASAVKIRAVVSEGEIVTGVFAREGDFLKDPVDTVVVPVQGRTAIL
                     RFTDLEPGEYALGLFQDLNGDERLDITLGGLSLEPWGVSNNKANLHEDPLWEDAKFTL
                     PPEGLSMVVHLLHERPGDR"
     gene            3351..3932
                     /locus_tag="VD791_07950"
     CDS             3351..3932
                     /locus_tag="VD791_07950"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HXV07947.1"
                     /translation="MLRNLLYQRGSFGRARLLSRCWIELRYRWHASRKGVWVAAFVAG
                     VAVGAFGVLIHSMVAHATRVRDLTCLARNVYYEARGEPAAGRLAVAEVTMNRVRSRRF
                     PNSVCEVVYQKNWDYLRRRYVSAFSWTEFDTTPHPERELWEQAWEAAEAVYDRRHPPV
                     LRGATHYHATYIKPSWARGQRPLAKIGRHVFYR"
     gene            4027..4494
                     /locus_tag="VD791_07955"
     CDS             4027..4494
                     /locus_tag="VD791_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV07948.1"
                     /translation="MSAITPKATHHANLPPLASRFVDVAALPWERGVHPGIETKTLLV
                     DRASGLLTMLLRMAPGAKLPDHEHVLIEQTYVLEGSLVCGEGVCRAGDYVWRPAGSRH
                     EAWAGPEGGLMLGMFQMPNRFFQTDGRPVDFLGNDWEKTWGAALERQEQAHLA"
     gene            4562..5797
                     /locus_tag="VD791_07960"
     CDS             4562..5797
                     /locus_tag="VD791_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HXV07949.1"
                     /translation="MTATINAQRLWDSLMQLAGIGATKKGGVCRLALSDLDGEARALF
                     IGWCKEAGCSVKVDAIGNIFARRPGQDAHAPAVVTGSHIDTQPSGGKFDGNYGVLAGL
                     EVVRTLNDLCLKTRAPIEVAVWTNEEGSRFTPGMMGSGVFVGEFALNAILQRADIDGV
                     SVGEALRGIGYAGAEPPGGRPLGAYFEAHIEQGPVLENERKVLGVVLGALGLRWYDVV
                     ISGQDAHAGPTPMRLRKDALVAAARIVAEVNALADRYQPDGRGTVGFMQVKPNSRNVV
                     PGEVRFSVDLRHAEDEPLERMEREMRAACERLAGAQRCTAGVERVACYPACRFDSGCV
                     SAVRQAAQRLGLSHMDIVSGAGHDAIYLARVCPTGMIFVPCEGGISHNEIENAKPEHL
                     AAGCNVLLQVMLERAGTVQ"
     gene            complement(5878..6546)
                     /locus_tag="VD791_07965"
     CDS             complement(5878..6546)
                     /locus_tag="VD791_07965"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXV07950.1"
                     /translation="MGKTIELTASDGHKLLAYRAEPAGKPRGGLIVAMEIFGVNSHIR
                     SVADGYAADGYLALAPALFDRVQRGYDTGYSQDEIQRGIAMIQKIDLEDTMKDVAAAL
                     QNTASAGKAGIVGYCWGGRVAWKAACSLSGLHCAVPYYGGGIPGMAGLEPRCPVMFHW
                     GETDHSIPLDQAKAFAATHAGQTHHFYPAGHGFNCDQRGSYDAAAAKLARQRTLEFLA
                     KHVG"
     gene            complement(6613..7419)
                     /locus_tag="VD791_07970"
     CDS             complement(6613..7419)
                     /locus_tag="VD791_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007583891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXV07951.1"
                     /translation="MPALNHPAQNLLDTLDGFRVEIDAEHERADIVLDRPPLNIVTMP
                     QREQLRLAFEALDADARVRVIVLRAAGEHFSSGGDIQGFLDASPEHVSQLAWNVAAPA
                     RCSKPVIAASRGYCFGVGFELSLACDFRIASDTALYALPEQKLGQIPGSGGSARLQKM
                     IGIARTKDVVMRSRRIGGRQACDWGIATECVPDAQLEAATAAMVDELRAFSPLAQRTA
                     KKLLNDTEDATLSVGIELEGHCYGRLRTSEDFREGVEAFHQKRKAVFKGR"
     gene            complement(7568..>7920)
                     /locus_tag="VD791_07975"
     CDS             complement(7568..>7920)
                     /locus_tag="VD791_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXV07952.1"
                     /translation="CGACTVRVDGTAVRACLMLAVQAEGRRVDTVEGLAGARGLSALQ
                     RAFKRHHALQCGYCTPGILMSATQFLERVPQPSEQQVREMLSGHLCRCTGYTNIVRAV
                     LDAAAQRRSPVSDA"
BASE COUNT         1196 a         2700 c         2822 g         1202 t
ORIGIN      
        1 accacggcgg ggcttgcagc gtcggccacc tgcaccactg cgcgcgagta agcgtcgagc
       61 agatccgcat cgtcgccggc agagcgcgcg ccgtcgctgg cagggcccac agccggcgct
      121 gcgggcgatg ccgacggcgc cggatcctcc accaggtgga gccagggcgc gctggcgagc
      181 aatgcggtca tggtgttctc ctcgtggatt cccgcaagga aagtgcgggc gcgatccagc
      241 gagttcaagc cggctaccgc cctcggggag cggctaccac cctccgccgc cgccgccacc
      301 cccgccgccg cccgacgagc cgcctccgcc gccccccgaa gaatcgccgg gcgcggtcga
      361 agccgacgag actgcggagt cgaaggaact ggaaagcgac gaggagaagc cgcccaggtc
      421 gttcgggtcc cagcgatcgc cgtgatacca gtccggcgag tggctctggg tcaggaacac
      481 gccggcgaac ttctccgacc agcgctgctc gacgccgagt gccagcgcga agggcagata
      541 ggcctcgaac agccccgggg tcttgcgcgg cgcccccgcg agcttcaact catcgccttc
      601 ggcgacatcg aggtacaggc gaaatccctc gatctcgtcc aggcgcctgc ggccttcgcg
      661 cgtgggtgcc ttgagcagct tgccgaacac cgcgttggcg atcaacgcga ccgcgccgag
      721 cgcgatcgtc gcccagcccc cgggcgtgag caggaaccag tcctggccgt agccgcccgc
      781 gatcgccggc gccaccacgc ccagcgcaac ggcgaggccc gtgagaaaga tgccgagcgc
      841 gtgccacttg ccgttgacgt ggaaaaagct cttcaggtac ttgcgcttga gcgccgcgtc
      901 gtgcgcgctc ctggcctgcc gcaggatcgc gtggttctca tcgtcgagca gcagcgtgtc
      961 gcgcgcgccg agcacctcgc gcagcaacgc gtgctcgtcg agcccgagcg gcgtctgcga
     1021 gccgcgcgtg cgctgcagcg tgtacttgcc tttcttcagg aagccaccgc cttcctgctc
     1081 gatcgtgaga tagcctttca ccgccagccc caggatcccg gccacgaagc agcggttgtc
     1141 gtaggccatg cgctgcagat agcgcatcga cgcaggcgtt tcgccctcgg gcggctggta
     1201 tcgcggaatg accacatcgc cggccggatc gcggcccacg cggcgccaca tcatcaggta
     1261 atagccgagc acgcagagca ggcccagcgc gccgaaggtc aggggccggt tgtcgcgcag
     1321 gaaatggcgc agcctggcgc tcgcagtcgg cgcgcttacc agccccttcg gccacatcac
     1381 cacgatggtt agcccttcgc gcgtggcgag cggccgcgtg gtctcgaatc tcgccacacc
     1441 gtcgcgtggc tgcgcgcggt agttctgcca cttcgcgccc atcggtccgg tgtagccctc
     1501 caggcgcacg gactcggcgg atgcaccggc gggaaggagc acctccgcgc tcgccgcgtc
     1561 gatcgggaaa tcccagtcca cgccggtcac gttccaatac agctcgtcgt gctccgggaa
     1621 gaagccgagc tggcggtcgg tgcgatagcg gatctcgtag gtgtattcgc caggcgagag
     1681 aacccggtcc ttgtcgccga tgtagagcgc cacgccgttg ctgcgccgtg ccgtgtgata
     1741 cggctcggtt gcaccgtcgc gccgcaccga cacgggagcg aagctcaccg tggtgcggtt
     1801 gccgcgccgg tcggtgtaca gcgtcgggaa ctcgcggaag atcccgcgcc ggatgcgatc
     1861 gccctcggcc cggacgcgga tggtctcggt caccagcagg ccgccctcgg gttggatctc
     1921 gacgcggctg tcgaaggaca ggatgcgctc gccggcgccg gccgcgccgg ccaggagcag
     1981 caggagagcg cacagcgctc gtcgcatcat cgcacctccg caggctgcgc ctcgccgggg
     2041 gcttgcagct cgaaatactc cgctgcccgg aacccgagca ggcgcgccac cagcagatcg
     2101 ggaaacgtgt ccacccgcgt gttgaggttg ttgaccgtgc cgttgtagta gcgccgggcg
     2161 tactggatct gatcctcggc ttgcgacagg ccgcgctgga gatcgagaaa gccctggctc
     2221 gcctttagct ccggatacgc ttcggccacc gccatgaggt cgtgcagatc gcgtccgagc
     2281 gcggcttcgg ggcggctctt gccggcgggg ccgctcgcct cctgcgcttg tgatcgcgtc
     2341 agcaccaggc gctccagcac gccgcgctcg tacccggcat gctgctggac cacttccacg
     2401 agcttgggga tcaggtcgtg gcggcgcttg agctggacgt ccacgtcgct ccaggccgcg
     2461 gccacgcgcg cacggtctcg caccagcagg ttgtaggcga cgatgcccca gacgatcagg
     2521 gcggcggcga tgacggcgac gatgacggtc atggcggcgc gtggcggttg tctcgccggc
     2581 cggcatgcag gatgcgcgcc aggcgcgagg tccggcccgc ggcggattgc ggccgcgacc
     2641 cgtgcagcgc tatcggtcgc cgggccgctc gtgcaggaga tgcaccacca tcgagagacc
     2701 ctcgggcggg agcgtgaact tggcgtcctc ccagagcgga tcctcgtgca ggttggcctt
     2761 gttgttcgac actccccagg gttccaggga caggccgccg agcgtgatgt cgagcctctc
     2821 gtcgccgttc agatcctgga acaggcccag cgcatactcg cccggctcga gatcggtgaa
     2881 gcgcaggatg gcggtgcggc cttgcaccgg caccacgacg gtgtcgaccg ggtctttcag
     2941 gaagtcgccc tcgcgggcga acaccccggt gacgatctct ccttcgctga ccaccgcgcg
     3001 gatcttgacg gcggaggcga aggcctcggc gctgtcgaac agcgccgcgc gcacctcccc
     3061 cttgcgcggc ttgacgttgc gcacttcgac ttgcagatcg gcggccgccg caccaccggc
     3121 cagcaggacg cacagcgctg cgaggccagc cagttgcgaa caggaccgcg aattgagcct
     3181 cgagaacatg gtttcggctc gtcgatgacg gccatggtag cgcgcatacg agaggactag
     3241 gaagggtttt ttcagcgcgc tgccgcgccg cacgacggcg tgattcttgc ctcgttggag
     3301 gtgtcgaacc cttgatgcgg ccgccggccg cggccggtca tccccaaccc atgctgcgca
     3361 acctcttgta ccagcgagga tcgttcggcc gtgcccggct gctgtcgcgg tgctggatcg
     3421 agctgcgcta tcgctggcac gcgagcagga agggcgtgtg ggtcgccgcg ttcgtggccg
     3481 gtgtggcggt gggcgcgttc ggcgtgctga tccattcgat ggtggcccac gctacgcgag
     3541 tgcgcgacct gacctgcctc gcgcgcaacg tgtactacga agcccgcgga gaacccgcgg
     3601 ccggccgcct cgccgtggcc gaagtcacca tgaaccgggt gcgctcgcgc cgctttccga
     3661 acagcgtgtg cgaggtcgtg tatcagaaga actgggacta cttgcgcagg cgctacgtca
     3721 gcgcgttttc ctggaccgag ttcgacacca ccccgcatcc cgaacgcgag ctgtgggaac
     3781 aggcctggga ggcggccgaa gcggtgtacg accggcgcca tccgccggtg ctgcgcggcg
     3841 cgacgcacta ccacgccacc tacatcaagc cgagctgggc gcgcggccag cggccgctcg
     3901 ccaagatcgg ccggcacgtg ttctacagat agggcgctgg ccggtgcgtc cgggcgcgca
     3961 cgccgcgccg tccggcagta cactcttgtc gggacccact cgagactctg cacggagaac
     4021 tgtccgatga gcgcgatcac gccgaaagcg acccaccacg ccaacctccc gccgcttgcg
     4081 tcgcgcttcg tcgacgtcgc ggcgctgccc tgggagcggg gggtgcatcc cgggatcgag
     4141 accaagacgc tgctggtgga tcgcgccagc ggcctgctca ccatgctgct cagaatggcg
     4201 ccgggcgcta agctgccgga ccacgagcac gtgctgatcg agcagacgta cgtgctcgaa
     4261 ggcagcctgg tgtgcggcga gggcgtatgc cgggccggcg actacgtgtg gcggccggcg
     4321 ggcagccggc acgaggcctg ggccgggccc gagggcggct tgatgctggg gatgttccag
     4381 atgcccaatc gcttcttcca aaccgacggg cgcccggtgg acttcctcgg caacgactgg
     4441 gagaaaacct ggggcgcggc gctcgagcgc caggagcagg cgcacttggc gtagccggtt
     4501 acggcgcgac cggtggcgcc gtgcctttgc ggcgggccgg ggccgggcgc cgggggatgc
     4561 gatgactgcc acgatcaacg cccagcggct gtgggattcg ctcatgcagc tcgccgggat
     4621 cggcgccacg aagaaaggcg gcgtgtgccg cctcgctctg tccgatctcg acggcgaggc
     4681 gcgcgcgctg ttcatcggct ggtgcaagga agcgggctgc agcgtcaagg tggatgcgat
     4741 cggcaatatc ttcgcgcgcc gccccggaca agatgcgcat gcgccggccg tcgtcacggg
     4801 cagccacatc gacacccagc cttccggcgg caagttcgac ggcaactacg gcgtgctggc
     4861 cgggctggag gtggtgcgca cgctcaacga tctgtgcttg aagacgcggg cgccgatcga
     4921 agtcgcggtc tggaccaacg aggaaggctc gcgcttcacg ccggggatga tgggctcggg
     4981 cgtgttcgtc ggcgagttcg cgctgaacgc catcctgcag cgcgccgaca tcgacggcgt
     5041 cagcgttggc gaagcgctgc gcgggattgg ctatgccggg gccgaaccgc cgggcggccg
     5101 gccgctgggc gcgtacttcg aggcgcacat cgagcagggg ccggtcctcg agaacgagcg
     5161 caaggtgctg ggcgtggtgc tcggcgccct cgggctgcgc tggtacgacg tcgtgatcag
     5221 cggccaggat gcgcacgctg gccccacccc gatgcgcctg cgcaaggatg cgctggtcgc
     5281 tgccgcccgc atcgtcgcgg aggtgaacgc gctcgccgat cgctaccagc ccgacggtcg
     5341 gggcacggtg ggattcatgc aagtgaaacc gaactcccgc aacgtggtcc cgggcgaagt
     5401 gcgcttctcg gtcgaccttc ggcatgccga ggacgagccg ctggagcgca tggagcgcga
     5461 gatgcgcgcc gcctgcgagc gcctggcggg cgcgcagcgc tgcaccgcgg gcgtggagcg
     5521 agtcgcctgc tatcccgcgt gccgcttcga ttccggctgc gtaagcgctg tgcgtcaggc
     5581 cgcgcagcgg ctagggctga gccacatgga catcgtcagc ggcgccggcc acgacgcgat
     5641 ctacctggcg cgcgtgtgcc ccaccggcat gatcttcgtg ccgtgcgagg gcggcatcag
     5701 ccacaacgag atcgagaacg cgaagccgga gcacctcgcg gccggctgta acgtgctgct
     5761 gcaggtgatg ctggagcgcg ccgggacggt gcagtgagga gataaacacc acaccgcccc
     5821 gccggcacca gaggcgggga acaggtagtt cgagtcgcgc ctcaggggcg cacctggcta
     5881 ccccacgtgc tttgccagga actccagcgt gcgctgccgc gcgagcttcg ccgcggcggc
     5941 gtcgtaggac ccgcgctgat cgcagttgaa cccgtgcccg gcggggtaga agtgatgcgt
     6001 ctggccggcg tgcgtcgcgg cgaacgcttt cgcctgatcg agggggatcg aatggtcggt
     6061 ctcgccccag tggaacatca cagggcagcg gggctcgagc ccggccatgc ccgggatccc
     6121 gccgccgtag tacggcacgg cgcagtgcag gccggagagg ctgcacgcgg ctttccatgc
     6181 caccctgccg ccccagcagt agccgacgat gccggccttg ccggcggagg cggtgttctg
     6241 cagcgccgcc gcgacgtcct tcatggtgtc ctcgaggtca atcttctgga tcatcgcgat
     6301 gccgcgctgg atctcgtcct gcgaatagcc ggtgtcgtaa ccgcgctgca cgcggtcgaa
     6361 cagcgccggg gcaagggcca gatagccgtc cgccgcgtac ccgtcggcca cgctgcgtat
     6421 gtggctgttg actccgaaga tctccatcgc cacgatcagg ccgccgcgcg gcttgccggc
     6481 gggttcggcg cgataggcga gcagcttgtg gccgtcggac gcggtgagct cgatggtctt
     6541 tcccatggcg gtctcctggt gagatagcgt ggctgtggaa tgatgctacg cgaagcggcc
     6601 gtcccgcgtg gatcagcgcc ccttgaacac cgccttgcgc ttctggtgga aggcctcgac
     6661 gccctcgcgg aagtcctcgg aagtgcgcag ccggccgtag cagtggcctt ccagctcgat
     6721 gccgacggaa agcgtggcgt cctcggtgtc gttgagcagc ttcttggcgg tgcgctgcgc
     6781 cagcggtgag aaagcgcgca gctcgtcgac catggccgcg gtcgccgcct cgagctgcgc
     6841 atcgggcacg cactcggtgg cgatgcccca gtcgcaggcc tggcggccgc cgatgcgccg
     6901 cgagcgcatc accacatcct tggtgcgcgc gatgccgatc atcttctgca ggcgggccga
     6961 gccgccggaa ccggggattt ggccgagctt ctgttccggc agcgcataca gcgcggtgtc
     7021 cgaggcgatg cggaaatcgc aggcgagcga cagctcgaag cccacgccga agcagtagcc
     7081 gcggctggcg gcgatcaccg gcttgctgca gcgcgcgggc gcggcgacgt tccaggcgag
     7141 ctgcgagacg tgttccggcg aggcgtcgag gaagccctgg atgtcgccgc cgctggagaa
     7201 atgctcgccg gcggcacgca ggacgatcac gcgcacgcgc gcgtccgcgt ccagcgcctc
     7261 gaacgccagg cgcagttgct cgcgctgcgg catggtgacg atgttgagcg gcgggcggtc
     7321 gagcacgatg tcggcgcgct cgtgctcggc gtcgatctcc acgcgaaagc cgtcgagggt
     7381 gtcaagcagg ttctgggcgg ggtggttcag cgctggcatg gtttcctctc aaacgggcgt
     7441 tggaccaccc cgcccgcttc gcgggcacac ctcctcgaag aggaggggaa cgcgccgctc
     7501 cccgcctctc acgaggcggg gtggcgccga aggcgacggg gcggtgtggt gtggagccgc
     7561 cggcggatca agcatccgac accggggagc ggcgttgcgc ggcggcgtcg agcacggcgc
     7621 gcacgatgtt ggtgtagccg gtgcagcggc acaggtgtcc ggacagcatc tcgcgcacct
     7681 gctgttcgct cggctgcggc acgcgctcga ggaactgggt cgcggacatc aggatgcccg
     7741 gcgtgcagta gccgcattgc agcgcgtgat gacgcttgaa ggcgcgctgg agcgcgctca
     7801 ggcctcgtgc gccggcgagc ccctccaccg tgtccacgcg ccggccctcg gcctgcaccg
     7861 cgagcatcag gcacgcgcgc accgccgttc cgtccacccg caccgtgcat gcgccgcaca
//
LOCUS       DASPGE010000247         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_64960, whole genome shotgun sequence.
ACCESSION   DASPGE010000247 DASPGE010000000
VERSION     DASPGE010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64960"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VD791_07980"
     CDS             <1..923
                     /locus_tag="VD791_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HXV07953.1"
                     /translation="CRVLADGYGSLFSLRRLAPELCAAGVEALPVMPPRLWGRKKLRL
                     DLRNHRKIAVIDGHIGYVGSQNIVDPDANRGLTNEELVARMTGPVVRQLQAVFLADRW
                     YETEAMPVDSQEELLPQVAETGRSPAQVMPSGPGYHGANTQQAMIAFIHAARARVVLT
                     TPYFMPDESFRLALRMAAERGVEVRLVLSRYSNKPIVHLAQESYYEELLEGGVHIHLY
                     YGNFLHAKHATIDDCVALVGSSNLDIRSFELNNEVGVLIYDPAVVAQLRAIQKRYFAA
                     SEEVRLEAWSRRNIAVRTAQNIARLADTLL"
     gene            1039..1737
                     /locus_tag="VD791_07985"
     CDS             1039..1737
                     /locus_tag="VD791_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HXV07954.1"
                     /translation="MITVRRSDARGHANHGWLDSRHTFSFADYYDPREMGFGSLRVIN
                     EDRVQPGKGFGTHPHRDMEIISYVLEGALEHKDSLGTGSVIRPGDVQRMSAGTGVLHS
                     EYNPSPREPVHFLQIWIEPSVRGVKPSYEQKAFSADDKRGRLRLVASPDGADGSVSIH
                     QDARVYAALLDGDEALTYAVPEGHRAYVHVARGSVQVNGQQLDAGDGAKIAAEAQLRF
                     ANGRHAELLLFDLA"
     gene            1753..>2028
                     /locus_tag="VD791_07990"
     CDS             1753..>2028
                     /locus_tag="VD791_07990"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HXV07955.1"
                     /translation="MLQTSPRLRDVTLVAPAHKAQPAPGLIVDRPFPGPALDRLDPFL
                     MLDHFGPEAIRQGDAGGLNPHPHRGFETVTIMLEGAMEHHDSQGNRGV"
BASE COUNT          332 a          682 c          686 g          328 t
ORIGIN      
        1 gctgccgcgt gctggccgac ggctacgggt cgttattttc cctgcggcgc ttggcgccgg
       61 agctgtgcgc agccggggtc gaagcgctgc cggtcatgcc cccgcgcctg tgggggcgca
      121 agaagctgcg cctcgatctg cgcaaccacc gcaagatcgc ggtgatcgac ggccacatcg
      181 gctatgtcgg ttcgcagaac atcgtcgatc ccgacgccaa ccgcgggctc accaatgagg
      241 agctggtggc gcgcatgacc ggaccggtgg tgcgccagct gcaggcggtg ttcctggccg
      301 atcgctggta cgagaccgaa gccatgccgg tcgactcgca agaggaactg ctgccgcaag
      361 tggccgagac cggccggtcc ccggcgcagg tcatgcccag cgggccgggc tatcacggcg
      421 cgaacacgca gcaggcgatg atcgcgttca tccatgcggc gcgcgcccgc gtggtgctca
      481 ccacgccgta cttcatgccg gacgaatcct tccggctggc gctgcgcatg gcggccgagc
      541 gcggcgtgga ggtgcggctg gtgctgtcgc gctattcgaa caagccgatc gtgcacttgg
      601 cccaggagtc gtactacgag gagctgctcg aaggcggcgt gcacatccat ctgtactacg
      661 gcaacttcct gcacgccaag catgccacca tcgacgattg cgtcgcgctg gtgggctcga
      721 gcaatctcga catccgctcc ttcgagctga acaacgaggt cggcgtgctg atctacgacc
      781 cggcggtggt ggcgcagctg cgcgccatcc agaagcgcta tttcgccgcc tcggaggagg
      841 tgcggctgga ggcctggagc cggcgcaata tcgcggtgcg cacggcgcaa aacatcgcgc
      901 gcctggcgga cacgttgctg tagcgccttc tgggcattcc gtttttctga acaactcctc
      961 cgaaaagtct cggcttgaag cccggaattt cggaccgatc ttgctttcaa acgatctgaa
     1021 cctttggagg ccattcggat gatcacggtt cgcaggagcg acgcgcgcgg ccacgccaat
     1081 cacggctggc tggacagccg ccacacgttc tctttcgccg actactacga tccgcgcgag
     1141 atgggcttcg gcagcctgcg cgtgatcaac gaggaccgcg ttcagccggg caagggcttc
     1201 ggcacccacc cgcaccgcga tatggagatc atcagctacg tgctggaggg cgcgctggag
     1261 cacaaggaca gcctgggcac cgggtcggtg atccgcccgg gcgacgtgca gcgcatgagc
     1321 gccggcaccg gtgtgctgca cagcgagtac aacccctcgc cgcgcgagcc ggtgcacttc
     1381 ctgcagatct ggatcgagcc cagcgtgcgc ggcgtgaagc cgagctacga gcagaaagct
     1441 ttctccgccg acgacaagcg cggccggttg cggctggtcg cctcgcccga cggtgccgac
     1501 ggctcggtct cgatccatca ggacgcccgg gtttacgccg ccttgctgga cggcgacgag
     1561 gcgctgacct atgccgtgcc cgaggggcac cgcgcgtacg tgcacgtcgc ccgcggcagc
     1621 gtgcaggtga acggtcagca gctcgacgcg ggcgacggtg ccaagatcgc ggcggaagcg
     1681 cagctgcgct tcgccaacgg ccgtcacgcc gagctgttgc tgttcgacct ggcttgaact
     1741 gaaggagcga cgatgttgca gacctccccc cgcttgcgcg acgtgacgct ggtcgcgccc
     1801 gcgcacaagg cccagcccgc acccgggttg atcgtggacc ggccgttccc gggcccggcg
     1861 ctcgatcgac tcgatccgtt cctgatgctc gaccacttcg gcccggaggc gatccggcag
     1921 ggcgacgccg gcggcctgaa cccgcacccg catcgcggct tcgagaccgt gaccatcatg
     1981 ctcgagggcg cgatggagca tcacgattcg cagggcaatc gcggcgtg
//
LOCUS       DASPGE010000248         8506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1119640, whole genome shotgun sequence.
ACCESSION   DASPGE010000248 DASPGE010000000
VERSION     DASPGE010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8506
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119640"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VD791_07995"
     CDS             complement(<1..899)
                     /locus_tag="VD791_07995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV07956.1"
                     /translation="MSANWRNPALVLACAAIVLALGLGIRQSFGLFLPADADLGWKRS
                     DFSFALAVSTLVWGLAQPFFGAWADRNGAGRVVAVGGLLFAAGLASMPLATTPLALTL
                     SAGLMVGLGLAATGFGIILGVVGRAFGPERCGVALGIASCGASFGQFLALPLGPLLTS
                     ALGWQTTLLVLAGIALATVPLALAMAERRAPVVAPGDRLSLPATLRQARDHAGFRYLT
                     AGFFVSGAQIAFILGHLPAYLLEVRMTPAVGATALALIGVFSIAGCIVSGYLGGRMSR
                     KVLLSGIHVLRALLVALFLGLPVS"
     gene            complement(959..1732)
                     /locus_tag="VD791_08000"
     CDS             complement(959..1732)
                     /locus_tag="VD791_08000"
                     /inference="COORDINATES: protein motif:HMM:NF021472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HXV07957.1"
                     /translation="MAQATASRDDESRMPVVQTIDAGAPLRWLAKGWRDLWRAPVASL
                     LVGVVFAAIGAFLVSFAWTHSHIAPTLVSGFLLVAPVLAINLYALSRQIERDGRPDPM
                     KALYAWRPNAGSIALFGLLLAFALIAWERISAILFALFYGGTVPDLENFLSDVLFSGR
                     YYQLLAAYFGIGAIIAALVFAVSVVSLPMLLDREVDTATAVITSVRAVANNPGPMILW
                     AAILAVLAAIGIATWMIGLVLIFPWLGHASWHAYRELVE"
     gene            1909..2421
                     /locus_tag="VD791_08005"
     CDS             1909..2421
                     /locus_tag="VD791_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003269964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HXV07958.1"
                     /translation="MRRRLYVICPDLEAAQRTMNDLLLARIDERHIHVLARPGAPMDQ
                     LHEASVLQKTDLVHGAELGLVIGGALGLLLGVVLVLVPLGGVQLQLVTVLITALGGAL
                     FGTWASSMVAASLPNTRLLAFAKDIEEGKYLMMVDVPFRRVEEIQSLLRKRHPEDKDG
                     GVEPTVPAFP"
     gene            2608..3507
                     /gene="cyoE"
                     /locus_tag="VD791_08010"
     CDS             2608..3507
                     /gene="cyoE"
                     /locus_tag="VD791_08010"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HXV07959.1"
                     /translation="MTTGVQQQISVSRLQQFLALTKPRVVSLIVFTAVIGMFLATPGM
                     VPLQALLFGTLGIGLVAGAAAAVNCLVEQKVDALMARTRARPLPMGQVSSLQTLLFAC
                     VVGGTGLTMLHALVNPLTMWLTLATFLGYAVIYTMILKPLTPQNIVIGGASGAMPPVL
                     GWTAVTGELHADALLLFLIIFAWTPPHFWALALYRKKEYAKAGVPMLPVTHGDRFTRL
                     HVLLYTLILFAVSLLPFSTRMSGVIYLAAALALGGVFLWYAIRIYTDYSDRLAHRTFR
                     YSIVYLSALFAALLLDHYFRIVI"
     gene            3589..4173
                     /locus_tag="VD791_08015"
     CDS             3589..4173
                     /locus_tag="VD791_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HXV07960.1"
                     /translation="MLRNLACAATACLVFASCSGGGPAFRSTDITGAELGRDFKLIDH
                     NGQPRTLADFRGKVLVVFFGFTHCPDVCPITLSELARALKRLGADAQRVQVVMITVDP
                     ERDTPEVLKQYVTALDPGFLGLTGDPQAIAETAREFKVFHQKSPGATPQAYSVDHSSG
                     TFVYDPAGRLRLLVGYGQGADVFAHDIGQLLRAG"
     gene            complement(4259..5122)
                     /gene="rpoH"
                     /locus_tag="VD791_08020"
     CDS             complement(4259..5122)
                     /gene="rpoH"
                     /locus_tag="VD791_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HXV07961.1"
                     /translation="MSTATLPVPMALPSAAGSLESYIQAVNRFPLLAEQEELRLARSY
                     RDNEDLDAARQLVLSHLRLVVSIARGYLGYGLGHADLIQEGNVGLMKAVKRFDPERGV
                     RLVSFAVHWIRAEIHEYILRNWRIVKIATTKAQRKLFFNLRSMKKGHGALTRDEIDAM
                     ASALRVKTSDVAEMETRLGGQDLALEPVGDDEEDGFGPIAYLSNPEDEPGRILEHEES
                     ARMRAQGLERALRSLDPRSRRIIEARWLRERDTLTLHDLASEFGVSAERIRQIEAQAM
                     KKMRGLIPVSA"
     gene            complement(5231..6151)
                     /gene="ftsX"
                     /locus_tag="VD791_08025"
     CDS             complement(5231..6151)
                     /gene="ftsX"
                     /locus_tag="VD791_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HXV07962.1"
                     /translation="MADGLRVWARQNGGAMRDATRRLARSPLATLLNVVVIGVALSLP
                     VGLYLGLTELQGVARQLSSDPQLSLFLDRDAGSDQAREIEERLKRHPIVASVRFVAKE
                     RALEDLKHGAGLAEVLDSLKENPLPDAFVVTVKGNSPDALEALHEEALVWPKVAHAQL
                     DSEWARKLDAVLRIGRTLALLLGVLLGAGLVAVTFNTIRLQILTRRDEIEVAKLIGAT
                     DAFVRRPFLYFGGLQGLAGGLMAWAVVAGALWFLNRELAGLSALYASGFRLRPLSGQD
                     ALLLFGVAAALGWLGAWLSVSRYLWQVEPR"
     gene            complement(6144..6809)
                     /locus_tag="VD791_08030"
     CDS             complement(6144..6809)
                     /locus_tag="VD791_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014110873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV07963.1"
                     /translation="MITFTHVTKRFPGGHEALRDVSFSVKSGELLTVVGRSGAGKTTL
                     LKLMSAIELPSTGNVVVSGQNTKALRPAAVPYLRRRFGLVFQDYKLLFDRNALDNVLL
                     PLHIVGLDGRDAVRRGRAALDKVGLLSREKAMPVTLSGGEQQRLCIARAVVNRPAVLL
                     ADEPTGNLDEAYAREIVELFRSFHQVGTTVVISTHDRAVQERLAGRTLTLVDGALTDS
                     AHG"
     gene            complement(6820..7803)
                     /gene="ftsY"
                     /locus_tag="VD791_08035"
     CDS             complement(6820..7803)
                     /gene="ftsY"
                     /locus_tag="VD791_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HXV07964.1"
                     /translation="MLGFLKGDKAARIENDEAQDSAGQAGGWLARLRSGLAGTRERFG
                     SRLGALLTGHAHIDENLYQELETLLLSADVGVASTGLLLGEMRKRVASERIEQPDALK
                     NVLKQCLLQVLEPVARPLSVEGKRPFVLLVAGVNGSGKTTSIGKLARYYQAQGKSVML
                     AAGDTFRAAAVEQLRTWGERNGVPVVSQQGGDPAAVVFDALQSAQARGVDLLVADTAG
                     RLPTQLHLMEELKKVKRVIARVVPDAPHEAMLVLDANTGQNALAQVQAFDDAIGLTGL
                     TVTKLDGTARGGTLVAIARYKPIPVRFIGVGEGIDDLRPFHARDFVDALFD"
     gene            8028..>8506
                     /locus_tag="VD791_08040"
     CDS             8028..>8506
                     /locus_tag="VD791_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXV07965.1"
                     /translation="MEILVKEDHRAPVVASMLWYRAGSMDEVSGSTGVAHVLEHMMFR
                     GTRTHAPGEFSKTIARAGGRDNASTGRDYTIYFQQLHRSQLPLALRLEADRMANLMLT
                     DEEFAREIRVVMEERRLRTDDQPRSLLYETFLASAYQAHPYRTPIIGWMRDLENMTLA
                     "
BASE COUNT         1349 a         2992 c         2852 g         1313 t
ORIGIN      
        1 gacacgggca gtccgaggaa caaggcgacg agcagggcgc gcaggacgtg aatgcccgac
       61 aggaggacct tcctgctcat gcgcccgccc agatagccgc tgacgatgca cccggcgatg
      121 ctgaacacgc cgatcagcgc cagcgccgtc gcgcccacag cgggagtcat ccgcacctcg
      181 agcaggtagg cgggcaggtg cccgaggatg aacgcgatct gggcgccgga tacaaagaag
      241 ccggctgtca ggtagcggaa cccggcgtga tcgcgggctt ggcgcagcgt cgcggggagc
      301 gacagccggt cgcctggcgc gactacagga gcgcgccgct ccgccatcgc cagcgccagg
      361 ggaacggtcg ccaaagcgat gccggccagc accagcagcg tggtctgcca gcccagcgcc
      421 gaggtcagca gcggccccag cggcagcgcc aggaattgtc cgaacgacgc gccgcaggat
      481 gcgatgccga gcgcgacacc gcaccgctcg gggccgaacg cgcggcccac cacgccgagg
      541 atgatgccga agccggtcgc ggccagtccc aggccgacca tcagcccggc gctcagcgtc
      601 agcgccaggg gcgtggtggc cagcggcatc gaagcgaggc cggcggcgaa cagcagtccg
      661 ccgactgcga cgacgcgccc ggcgccgttg cggtcggccc atgcgccgaa gaacggctgc
      721 gccagccccc agaccagagt cgacaccgcc aaggcgaagg agaagtcgct gcgcttccac
      781 cccagatcgg cgtccgcagg cagaaacaaa ccgaagctct ggcggatacc gaggccgagc
      841 gcgaggacga tcgcggcgca ggcgaggacc agggccgggt tgcgccagtt ggccgacaaa
      901 ccgctgctac ctcgacccga tgcggccttg actcggcgca gccccgttca cccggacgtc
      961 actcgaccag ctcgcggtag gcatgccacg aggcatgccc cagccacggg aagatcagca
     1021 ccagcccgat catccaggtg gcgatgccga tcgcggcgag cacggccagg atcgcggccc
     1081 acaggatcat cggcccgggg ttgttggcca ccgcgcgcac gctggtgatc accgccgtgg
     1141 cggtgtcgac ctcgcggtcg agcagcatcg gcagcgacac caccgagacc gcgaacacga
     1201 gcgccgcgat gatcgcgccg atgccgaagt acgccgccag caactgatag tagcgccccg
     1261 agaacagcac gtcgctcagg aagttctcca ggtccggcac cgtgccgccg tagaacagcg
     1321 cgaacaggat cgccgagatg cgctcccagg cgatcagcgc gaaggcgagc agcagaccga
     1381 acagcgcgat cgagcccgca ttcggacgcc aggcgtacag cgctttcatc gggtccgggc
     1441 ggccgtcccg ctcgatctgc cgcgacaatg cgtagaggtt gatggccagg accggcgcca
     1501 ccagcaggaa tccgctcacc agcgtcggtg cgatgtggct gtgggtccag gcgaaggaca
     1561 ccaggaacgc tccgatcgca gcgaagacca ccccgaccaa gagacttgcg accggcgcgc
     1621 gccacaggtc gcgccagccc ttcgcgagcc agcgcaacgg cgcgccggcg tcgatcgttt
     1681 gcaccaccgg catgcgcgat tcgtcgtccc ggcttgcggt cgcctgcgcc atcgttcctc
     1741 ctgtcgaagg gtcgtcctgc ggccgccaca atgcgctcga ggcggcggct cgtcaaccgg
     1801 cggtgcatcg ttcctaggcc ttgccaaccg caacaaagtc tagaatgata attgcttcga
     1861 ttatgtcggc agtcgctatc ccgcgttcct gacccgagga ggtttgccat gcggcgcagg
     1921 ctgtacgtga tctgtcccga tctggaggcg gcgcaacgga cgatgaacga tctgttgctc
     1981 gcccgcatcg acgaacgtca catccatgtc ctggccagac ccggggcgcc catggaccag
     2041 ctgcacgagg ccagcgtcct gcagaagacc gacctggtgc acggcgccga actggggctg
     2101 gtcatcggcg gcgcactcgg gctgctgctg ggcgtggtcc tggtgctggt tcctctcggg
     2161 ggcgtgcagt tgcagctggt gacggtgctg atcaccgcgc tgggcggcgc gctgttcggc
     2221 acctgggcct cgagcatggt tgcggcgagt ctgcccaaca ccaggctgct tgccttcgcc
     2281 aaggacatcg aggaagggaa gtacctgatg atggtcgacg ttcctttccg gcgcgtcgag
     2341 gagatccagt cgctgctgcg taaacgccat ccggaagaca aggacggcgg agtcgagccg
     2401 acggtcccgg cgtttccctg atccgcgctc tcgcccggtc cgtcgagccg gacgtccgcc
     2461 cgatcgaggg cgcaagtcga gccccgcgat tcttcgcggg gcttgttcgt ttctgaagac
     2521 ggaccgcagc ggctcgcggg cggggatggt acactcccgt cccgccggcg ccttccgccg
     2581 gaacttgctt gcgcgactgg ttccgccatg acgaccggcg tacaacaaca gatctccgtt
     2641 tcccgcctgc agcagtttct ggcgctcacc aagccgcgcg tcgtttccct gatcgtcttc
     2701 actgcggtga tcggcatgtt cctggccacg cccggcatgg tgccgctgca ggcgctgctg
     2761 ttcggcacgc tcggcatcgg actggtcgcc ggcgccgcgg ccgcggtcaa ctgcctggtg
     2821 gagcagaagg tcgatgcgtt gatggcgcgc acgcgcgcgc ggcccctgcc catggggcag
     2881 gtgagctccc tgcagacgct gctgttcgcc tgcgtggtcg gcgggaccgg gctcaccatg
     2941 ctgcacgcgc tggtcaaccc gctgacgatg tggctgacgc tcgccacctt tctcggctac
     3001 gcggtcatct acaccatgat cctcaagccg ctcacgccgc agaacatcgt gatcggcggc
     3061 gcctccggcg ccatgccgcc ggtgctgggc tggactgcgg tcaccggcga gctgcacgcg
     3121 gacgcgctgc tgctgttcct gatcatcttc gcgtggacgc cgccgcactt ctgggcgctg
     3181 gccctgtacc gcaagaagga atatgccaag gccggcgtgc cgatgctgcc ggtcacccac
     3241 ggcgaccgtt tcacgcgcct gcacgtgctg ctctataccc tgatcctgtt cgcggtcagc
     3301 ctgctgccgt tcagcacgcg catgagcggc gtgatctatc tggcggcggc gctcgcgctg
     3361 ggcggtgtgt tcctgtggta tgcgatcagg atctacaccg actacagcga tcggctggcg
     3421 cacaggacct tccgctactc catcgtgtac ctgtcggcgc tgttcgcggc gctgctgctg
     3481 gaccactact tccgtatcgt catctagccg acccgtcccg gcacggcgac gcgctcctgc
     3541 gcaggggccg agccgggacc ggcgcggcgt cccgggataa ccaccaccgt gctacgcaac
     3601 cttgcctgcg ccgcgaccgc ctgtctcgtg ttcgcgagct gcagcggcgg cgggcccgcg
     3661 ttccgctcca ccgacatcac gggcgcggaa ctgggccgcg acttcaagct gatcgatcac
     3721 aacgggcagc cgcgcacgtt ggcggatttc cgcggcaagg tcctggtggt gttcttcggt
     3781 ttcacgcatt gccccgacgt ctgcccgatc acgctttcgg agctggcccg cgcgctgaag
     3841 cggctgggcg ccgatgcgca gcgcgtgcag gtggtgatga tcaccgtgga cccggaacgc
     3901 gacacgcccg aggtcctgaa gcaatacgtg acggcgctcg atcccggctt cctggggctg
     3961 acgggcgacc cccaagccat cgcggagacc gcacgcgagt tcaaggtctt ccaccagaag
     4021 agcccgggcg cgacgccgca ggcctactcc gtggaccatt cctccggcac cttcgtctac
     4081 gatccggccg gacggttgcg cctgctcgtg ggatacggcc agggcgcgga cgtgttcgcg
     4141 cacgacatcg gccagctcct gcgcgcgggg tgagcaggcc gcaatcatcg gccgcttgcg
     4201 gaaacaaaaa cgccgcgccc cgagggagcg gcggcgggcg gcgtcgcttg cttcgccgtc
     4261 aggcggatac cggaatcagc ccgcgcatct tcttcatcgc ctgcgcctcg atctgtcgga
     4321 tgcgctcggc ggacacgccg aactcgctcg ccagatcgtg cagggtgagc gtgtcccgct
     4381 cccgcagcca gcgcgcttcg atgatgcggc ggctgcgcgg atcgaggctt cgcagagcgc
     4441 gttcgaggcc ctgtgcacgc atgcgcgcac tttcttcgtg ctcgaggatg cggccgggct
     4501 cgtcttccgg attggacaga taggcgatcg gcccgaagcc atcctcctcg tcgtcgccga
     4561 ccggttccaa ggccaggtcc tggccgccca ggcgggtttc catctcggcc acatccgagg
     4621 tcttcacccg cagcgcgctc gccatcgcgt cgatctcgtc ccgggtgagc gcgccatggc
     4681 ccttcttcat gctgcgcagg ttgaagaaca gcttgcgttg cgccttggtg gtcgcgatct
     4741 tgacgatgcg ccagttgcgc aggatgtatt cgtggatctc ggcccggatc caatgcacgg
     4801 cgaacgacac caggcgcacg ccgcgctcgg ggtcgaagcg cttgaccgcc ttcatcagac
     4861 cgacgttacc ttcctggatc aggtcggcgt gccccaggcc gtaaccgaga tagccgcggg
     4921 cgatggaaac caccagccgc aggtgcgaaa gcacgagctg gcgtgcggcg tccaggtcct
     4981 cgttgtcgcg gtaggatcgg gccaggcgca attcctcctg ctcggccagc aaggggaagc
     5041 ggttcaccgc ctggatgtag ctctccaggc tgccggctgc cgacggcaag gccatgggga
     5101 cgggcagggt agcggtgctc atgctgaagc ctcctcgatg cgcaaatttt agcactcggt
     5161 acatcagagt gccaggaggg cctcgagttc cggcaatttc cgagtatttt tgtcggctat
     5221 caggttgacg ctaacgaggc tcgacctgcc agagatagcg gctcaccgag agccaggcgc
     5281 cgagccagcc gagcgccgcg gcgactccga acaacagcag ggcatcctgt ccggagaggg
     5341 ggcgcaaccg aaagccggag gcgtacagcg cggacaggcc ggcgagctcg cggttgagaa
     5401 accacagcgc cccggccacc accgcccagg ccatcaggcc gccggccagc ccctgcagcc
     5461 cgccgaagta caggaacgga cggcggacga aggcatcggt cgcgccgatc agcttcgcca
     5521 cctcgatttc gtcgcgccgg gtcaggatct gcagccggat ggtgttgaag gtcaccgcca
     5581 ccaggccggc cccgagcagc accccgagca gcagcgccag ggtgcggccg atgcgcagca
     5641 cggcgtccag cttgcgcgcc cactccgagt cgagctgcgc gtgcgccacc ttgggccaga
     5701 ccagggcctc ttcgtgcagc gcctccagcg catcggggga gttgcccttc accgtcacga
     5761 cgaaggcgtc gggcagcggg ttttccttca gcgagtcgag cacctcggcg agcccggcac
     5821 cgtgcttgag atcctcgagc gcgcgctcct tggcgacgaa gcgcacgctg gcgactatgg
     5881 ggtgacgctt caggcgctcc tcgatctcgc gcgcctggtc gctgcccgcg tcgcgatcca
     5941 ggaacaggct gagctgggga tcgctggaca gctgccgggc cacgccctgc aactcggtga
     6001 gtcccaggta cagacccacc ggcaggctca gggcgacccc gatcaccacc acgttgagca
     6061 gcgtggcgag cgggctgcgc gcgaggcgcc gcgtcgcgtc ccgcatcgcg ccgccgttct
     6121 gccgcgccca gacgcgcagg ccgtcagcca tgcgccgaat ccgtcagcgc gccgtccacc
     6181 agcgtcagcg tgcggccggc cagccgctcc tgcaccgcgc ggtcgtgggt ggagatcacc
     6241 accgtcgtgc ccacctggtg aaacgagcgg aacagctcga cgatttcgcg cgcgtaggcc
     6301 tcgtcaaggt tgccggtggg ctcgtccgcc agcagcaccg ccggccggtt gaccacggcg
     6361 cgcgcgatgc acaggcgctg ttgctcgccg ccggacaacg tcaccggcat cgccttctcc
     6421 cggctcagca ggcccacctt gtccagcgcc gcgcgcccgc ggcgcacggc gtcgcgcccg
     6481 tcgaggccga cgatgtgcag cggcagcagc acgttgtcca gcgcgttgcg gtcgaacagc
     6541 agcttgtagt cctggaacac caggccgaac ctgcgccgca ggtagggaac cgccgccggg
     6601 cgcagcgcct tggtgttctg gccgctcacc acgacgttgc cggtgctcgg cagctcgatc
     6661 gcggacatca gcttgagcag cgtcgtcttg cccgctccgg agcggccgac cacggtgagc
     6721 agctcgcccg acttgaccga gaagctcacg tcccgcaacg cctcgtggcc gccggggaaa
     6781 cgcttggtga cgtgggtgaa agtgatcacc gtccggggtt cagtcgaaca gcgcgtccac
     6841 gaagtcgcgt gcgtgaaacg ggcgcagatc gtcgatgcct tcgccgacgc cgatgaagcg
     6901 caccgggatc ggcttgtaac gcgcgatcgc caccaaagtg ccgccgcgcg cggtgccgtc
     6961 gagcttggta acggtgaggc cggtcaggcc tatggcgtcg tcgaaggcct gcacctgggc
     7021 gagcgcgttc tgcccggtgt tggcgtccag caccagcatc gcttcgtgcg gtgcatccgg
     7081 gacgacgcgc gcgatcacgc gcttgacctt tttcagctcc tccatcaggt gcagctgcgt
     7141 gggcagccgc cccgcggtgt ccgcgaccag cagatcgacg ccgcgcgcct gcgccgactg
     7201 cagcgcgtcg aacaccaccg ccgcggggtc gccgccctgc tggctcacca ccgggacgcc
     7261 gttgcgctcg ccccaggtcc tgagctgctc cacggcggcg gcgcgaaacg tatcgccggc
     7321 cgcgagcatc acgctcttgc cctgcgcctg gtagtagcgg gcgagcttgc cgatcgaggt
     7381 ggtcttgccc gagccgttca cgcccgccac cagcagcacg aacggacgct tgccctcgac
     7441 gctcagcggc cgggccaccg gttcgagcac ctgcagcagg cactgcttga gcacgttctt
     7501 gagcgcatcc ggctgctcga tgcgctcgct cgccacgcgc ttgcgcatct cgcccagcag
     7561 cagcccggtg gaggccacgc ccacgtcggc ggacagcagc agcgtctcga gctcctggta
     7621 caggttctcg tcgatgtgcg cgtgcccggt gagcagcgcg ccgagccggc tgccgaatct
     7681 ttcgcgcgtg ccggcgaggc cgctgcgcag ccgcgcgagc cagccgcccg cctgccctgc
     7741 gctgtcctgt gcctcgtcgt tctcgatccg ggccgccttg tcgcccttga gaaaacccaa
     7801 catactgcct gcgtgtccaa gtcgaaagcg cgcgcagatg cgcggcgatg gctggattct
     7861 agcagggtgc tccagtggtg atgattcctt cctcggatcg cgtccgttgc gcggcgcacg
     7921 ccatcttttc ctcccgtctc gcgctgcggg gcgcgctcgc ggtcgcgtgc gcgtgcccgg
     7981 ccgtgcccgc agccgccgac ctgcaacggc accggctgga caacggcatg gagatcctgg
     8041 tcaaggaaga tcatcgtgcg ccggtggtgg cctcgatgct gtggtaccgc gcgggcagca
     8101 tggacgaggt cagcggcagc accggtgtgg cgcacgttct cgagcacatg atgttcaggg
     8161 gcacgcgcac gcacgccccc ggcgagttct ccaagaccat cgcgcgcgcc ggcgggcgcg
     8221 acaacgcctc caccggccgc gactacacca tctatttcca gcagctgcac cgctcgcagc
     8281 tgcccctcgc cctgcgcctg gaggccgacc gcatggccaa cctgatgctc acggacgagg
     8341 agtttgcgcg cgagatccgg gtggtgatgg aggagcggcg cctgcgcacg gacgaccagc
     8401 cgcgttcact gctgtacgag acgtttctcg ccagcgcgta tcaagcgcac ccctaccgca
     8461 cgccgatcat cggttggatg cgggacctgg agaacatgac gctggc
//
LOCUS       DASPGE010000249         5684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_417222, whole genome shotgun sequence.
ACCESSION   DASPGE010000249 DASPGE010000000
VERSION     DASPGE010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417222"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VD791_08045"
     CDS             <1..476
                     /locus_tag="VD791_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006494379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXV07966.1"
                     /translation="RASDYADLDQMYVKSRAQGFGAEVKRRILVGTYVLSHGYYDAYY
                     LKAQKVRRLIAQDFARAFKRCDLILGPTAPSVAFDLGAKSDDPVQMYLSDIYTVSVNL
                     AGLPGLSIPCGFGAGGRPVGLQMVGNYFQEARMLNAAHRYQLATDWHLRVPPGIE"
     gene            479..1909
                     /gene="gatB"
                     /locus_tag="VD791_08050"
     CDS             479..1909
                     /gene="gatB"
                     /locus_tag="VD791_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HXV07967.1"
                     /translation="MNWEIVIGLETHAQLSTQSKMFSEAPTAFGAAPNTQANAVDIAL
                     PGVLPVANRGAVERAIRFGLAVGGTVHQRSVFARKNYFYPDLPKGYQISQYELPIVTG
                     GSVRIRSGETEKRVRLTRAHLEEDAGKSLHEDFHGMSGIDLNRAGTPLLEIVSEPDMR
                     SADEAVAYAKALHALVRWIDICDGNMQEGSFRCDANVSVRPAGSDRLGTRCEIKNLNS
                     FRFLERAILFEARRQIEILEDGGAVRQETRLYDPERDETRSMRSKEDAPDYRYFPDPD
                     LLPLAVSDAWIAEVRAALPELPEAMRERFVREHGLPAYDAGLLTADREIAAYFEALVR
                     ALPQQPKLAANWVMGELSARLNRDDLDIARSPVDSTALAGLLARVADGTLSGKLAREV
                     FDAMWEGQGSADRIIAARGLQQISDSGELERIVAAVLDANPAQVAEYRAGKEKAFNYL
                     VGQAMKASKGKANPAQLSEILKRKLS"
     gene            complement(1944..2624)
                     /locus_tag="VD791_08055"
     CDS             complement(1944..2624)
                     /locus_tag="VD791_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HXV07968.1"
                     /translation="MSLRDHTTTDTGRLLPARAAALLLGLATVLVLAGAAGAAQPGRI
                     YKWVDEHGVTHYGQHIPPEYRDQAATEMTKRGVTVNRIDPVTTPEERRLAQERALREK
                     EERKRLAEQRRRDTALMNTYSSAREIDDARERSLSAPMQALTALEPRFKRANERLQGL
                     NRQAADLQRAGKPVPEFLNEDIAAAKHEVEQLQAERQRHESYIAGIRTRFDADKQRYI
                     ELTQLGPR"
     gene            complement(2596..3282)
                     /gene="pyrE"
                     /locus_tag="VD791_08060"
     CDS             complement(2596..3282)
                     /gene="pyrE"
                     /locus_tag="VD791_08060"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HXV07969.1"
                     /translation="MRTPSDQRYRREFIEFAIDTRVLRFGEFKTKAGRLSPYFFDAGL
                     FNDGESLRRLGQFYAKALTSAGVAFDLLFGPAYKGIPLVTATAVALAETGRNVPICFN
                     RKEAKDHGEGGLTVGAPLAGRVLIVDDVISAGTSVRESVEIIRAGGATPCAVVIALDR
                     MERGSGPLSAAQEVQQIYQIPVLSIADLDDLVACLASRGGMARELQLVARYRTQYGAA
                     QHVAQGSHHD"
     gene            3275..4048
                     /locus_tag="VD791_08065"
     CDS             3275..4048
                     /locus_tag="VD791_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HXV07970.1"
                     /translation="MRIITLNLNGIRSAVNKGFLPWLMRQQADVACVQEIKAQPGDIV
                     DATRNPDALLGYFHCAQRRGYSGVGIYCRRAPDEVREGVGIPEIDAEGRYLEARFGDL
                     SVISFYVPSGSSSEERLAIKFKFLERVIEPLHKLAASGREIVICGDWNIAHKEIDLKN
                     WRANQKNPGFLPEERAWLSGVFERLGYVDVFRRIDPRPEQFTWWSNRGQAWAKNVGWR
                     LDYQIATPGIAARARAASIYKARRFSDHAPLTIDYDYTL"
     gene            4119..5519
                     /locus_tag="VD791_08070"
     CDS             4119..5519
                     /locus_tag="VD791_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV07971.1"
                     /translation="MSAAPPARRWLQDFKVYAEPPVLRMLLLGFSAGLPLLLVLGTLS
                     YRLREAGIDRTTIGFLSWVGLAYGFKWLWAPLVDRLPLPLLTRTLGRRRSWLLFAQTG
                     ILVALAGMALTDPLDDLQRLVLFALLAAFASATQDIALDAFRIESAALERQAAMAAAY
                     QVGYRGAMICASAGALWIAAAFDPDERTYQHGPWLAAYLVMAALMLIGMITVLLSPEP
                     RITVSAETVAREHEAARRLAAGGLPPPVARLLAWFYSAVASPFIDFVLRYRWQALLLL
                     ALIATYRVSDIVLGVMSNPFYRDMGFTKDEVATVSGVYGVLMTLAGSALGGVLSLRFG
                     VVRVLFLGAVLSAATNLMFAWLATRGHDLPGLVMVISADNLSAGIASAAFVAYLSGLT
                     NVAYSATQYALFSSVMLLLPKFIAGWSGMAVDGFGYPAFFTGTALLGMPVLLLTWLVW
                     RESKRAQPAAPSTTPA"
BASE COUNT          841 a         1965 c         1941 g          937 t
ORIGIN      
        1 tgcgggcctc cgactacgcg gacctggacc agatgtacgt gaagagccgt gcccagggct
       61 tcggcgcgga ggtcaagcgc cgcatcctgg tgggcaccta cgtcctgtcg cacggttact
      121 acgacgccta ctatctgaag gcgcagaagg tccggcgcct gatcgcgcag gacttcgcgc
      181 gcgcgttcaa gcgctgcgac ctgatcctgg gccccacggc gccgagcgtg gcgttcgatc
      241 tgggcgcgaa gagcgacgac ccggtgcaga tgtacctgtc ggacatctac accgtctcgg
      301 tcaacctggc ggggcttccc ggcctgtcca tcccgtgcgg gttcggcgcc ggcgggcgtc
      361 cggtcgggct gcagatggtg ggcaactact tccaggaggc gcgcatgctg aacgccgcgc
      421 accgctacca gctggcgacc gactggcact tgcgcgtgcc tcccggcatc gagtgaacat
      481 gaactgggaa atcgtcatcg ggttggagac ccacgcccag ctctcgacgc aatcgaagat
      541 gttctcggag gcgccgaccg ctttcggcgc ggcgcccaac acgcaggcca acgcggtgga
      601 catcgcgctg ccgggggtgc tgccggtggc caaccgcggc gcggtggagc gcgccatccg
      661 cttcggcctc gcggtcgggg gcacggtgca ccagcgctcg gtgttcgcgc gcaagaacta
      721 cttctacccc gatctgccga agggctatca gatcagccag tacgagctgc cgatcgtgac
      781 cggcggcagc gtgcgcatcc gctcaggtga gaccgaaaag cgggtgcggc tcacccgcgc
      841 gcatctagag gaggacgccg gcaagtccct gcacgaggac ttccacggca tgagcggcat
      901 cgacctcaat cgcgccggca cgccgctgct ggagatcgtg tccgagccgg acatgcgctc
      961 ggcggacgaa gcggtggcct acgccaaggc gctgcacgcg ctggtgcgct ggatcgacat
     1021 ctgcgacggc aacatgcagg agggatcgtt ccgctgcgac gccaacgtct cggtgcgccc
     1081 ggcgggcagc gaccgcctcg gcacccgctg cgagatcaag aacctcaact ccttccgctt
     1141 cctggagcgc gccatcctgt tcgaggcgcg gcgccagatc gagatcctgg aagacggcgg
     1201 cgcggtgcgc caggagaccc gcctgtacga tccggagcgc gacgagacgc gctccatgcg
     1261 ctcgaaggag gatgcgccgg actaccgcta cttccccgac cccgacctgc tgccgctggc
     1321 ggtcagcgac gcctggatcg ccgaggtgcg cgcggcgttg cccgagctgc ccgaagccat
     1381 gcgcgaacgg ttcgtgcgcg agcacgggct gcccgcgtac gacgccgggc tcctcaccgc
     1441 ggaccgcgag atcgcggcgt acttcgaagc gctggtgcgc gcgctgccgc agcaacccaa
     1501 gctcgccgcc aactgggtga tgggcgagct gagcgcgcgc ctgaatcgcg acgacctcga
     1561 catcgcccgc tcgccggtcg acagcaccgc cctggccggg ctgctggcgc gggtggcgga
     1621 cggcacgttg tccggcaagc tcgccaggga ggtcttcgac gcgatgtggg agggacaggg
     1681 cagcgccgac cggatcatcg ccgcgcgcgg gctgcagcag atctccgaca gcggcgagct
     1741 ggaacggatc gtcgccgcgg tgctggacgc caatcccgcg caggtagccg aataccgggc
     1801 gggaaaggag aaggcgttca actacctggt gggccaggcg atgaaggcca gcaagggaaa
     1861 ggcgaacccc gcgcagctga gcgagatcct gaagcgcaag ctgagctagc ccggatactc
     1921 cgcgtcctga tgaaaaatcc gggctagcgc gggccgagct gggtcagctc gatgtagcgc
     1981 tgcttgtccg cgtcgaagcg ggtgcgaata cccgcgatgt agctctcgtg gcgctggcgc
     2041 tcggcctgca gctgctccac ctcgtgcttc gcggccgcga tgtcctcgtt caggaactcg
     2101 ggcaccggct tgcccgcgcg ctgcaggtcg gctgcctgcc ggttcaagcc ctgcaagcgc
     2161 tcgttcgccc tcttgaagcg cggctccagg gcggtcagcg cctgcatcgg cgcgctcagg
     2221 ctgcgctcgc gcgcgtcgtc gatctcgcgg gcgctggagt acgtgttcat cagcgcggtg
     2281 tcgcgccgcc gctgctcggc caggcgcttc cgctcctctt tttcgcgcag cgcccgctcc
     2341 tgggccagtc tgcgctcctc gggcgtggtc accgggtcga tgcgattgac cgtgacgccg
     2401 cgcttggtca tctcggtcgc ggcctggtcg cgatattccg gcggaatgtg ctggccgtag
     2461 tgcgtcaccc cgtgctcgtc cacccacttg tagatgcggc cgggctgtgc ggcgccggcg
     2521 gcgccggcca gcaccagcac ggtcgccagg ccgagaagca gcgcggccgc gcgcgctgga
     2581 agcagacgcc ccgtgtcagt cgtggtgtga tccctgagcg acatgctgag cggctccgta
     2641 ttgagtgcga taacgggcca ccaattgcaa ctcgcgtgcc attccgccgc ggctcgcgag
     2701 gcacgccacc aggtcgtcca ggtcagcgat gctgagcacg ggaatttgat aaatttgttg
     2761 aacctcctgg gccgccgaca gcggaccgct gccgcgctcc atgcggtcga gcgcgatcac
     2821 cacagcgcac ggcgtcgcgc cgccggcgcg gatgatctcc accgactcgc gcaccgaggt
     2881 gccggccgag atgacatcgt cgacgatcag cacgcgcccg gccagcggcg cgcccacggt
     2941 caggccgccc tcgccgtgat ccttcgcttc cttgcgattg aagcagatcg ggacgtttcg
     3001 ccccgtttcg gcgagcgcca cggcggtggc ggtcaccagc ggaatgccct tgtacgccgg
     3061 cccgaacagc aggtcgaagg ccaccccagc cgaggtcagg gcttttgcgt agaattgccc
     3121 cagccggcgc agggactctc cgtcgttgaa caggccggca tcgaagaaat agggcgacag
     3181 acggccggcc ttggtcttga actcgccgaa acgcagaaca cgggtatcga tcgcgaactc
     3241 gatgaactcc cggcggtacc gctgatcgga cggcgtgcgc atcatcacgc tcaatcttaa
     3301 cggcatccgc tcggccgtca acaagggttt cctgccttgg ctgatgcggc aacaggccga
     3361 cgtggcctgc gtacaggaaa tcaaggccca gcctggcgat atcgtcgacg ccacgcgcaa
     3421 cccggacgcg ctcctcggct atttccattg cgcgcagcgg cgcggctaca gcggcgtggg
     3481 catctactgc cggcgggcgc cggacgaagt gcgcgagggt gtgggcattc cggagatcga
     3541 cgccgagggc cgctacctgg aagcccgctt cggcgacctg agcgtgatct cgttctacgt
     3601 gccgtccggc tccagctccg aggagcggct ggcgatcaag ttcaagttcc tggagcgcgt
     3661 gatcgaaccg ctgcacaagc tcgccgccag cggccgcgag atcgtgatct gcggcgactg
     3721 gaacatcgcc cacaaggaga tcgacctcaa gaactggcgc gccaaccaga agaacccggg
     3781 ctttctgccc gaggaacgcg cctggctctc gggggtattc gagcgcctcg gctacgtgga
     3841 cgtcttccgc cgcatcgacc cgcgcccgga gcagttcacc tggtggtcga accgcggcca
     3901 ggcctgggcg aagaacgtgg gctggcggct ggactatcag atcgccacgc cgggcatcgc
     3961 ggcgcgggcc agggcggcgt ccatctacaa ggcgcggcgc ttctccgatc acgcgccgct
     4021 caccatcgac tacgactaca ccctgtgacg acgacgctgg acgccgactc catgcaccgg
     4081 cgtcgaggcg ccggccaacc ggctcgggtg acaccggcgt gagcgcggca cccccggcgc
     4141 gccgctggct gcaggacttc aaggtctatg ccgagccgcc ggttctgcgc atgctgctgc
     4201 tcggcttctc cgcggggctg cccctgctgc tggtgctggg cacgctgtcc taccgcctgc
     4261 gtgaagcggg catcgaccgg accaccatcg gcttcctgag ctgggtgggc ctagcctacg
     4321 gcttcaaatg gctgtgggcg ccgctggtgg accgtctgcc gctgccgctg ctcacgcgca
     4381 cgctcggccg gcggcgcagc tggttgctgt tcgcgcagac cggcatcctg gtagcgctcg
     4441 ccggcatggc gctgaccgac ccgctggacg atctgcagcg cctggtgcta ttcgcattgc
     4501 tcgcggcttt cgcctccgcc acccaggaca tcgcgctgga cgcctttcgc atcgagtcgg
     4561 cggcgctcga gcggcaagcc gcgatggccg ccgcctatca ggtcggctac cgcggcgcca
     4621 tgatctgcgc ctcggccggt gcgctgtgga tcgcggcggc gttcgacccg gacgagcgca
     4681 cgtatcagca tggcccctgg ctggcggcct acctcgtcat ggctgcgctg atgctgatcg
     4741 gcatgatcac cgtgctgctc tccccggagc cgcgcatcac ggtgtcggcc gagacggtgg
     4801 cgcgcgagca cgaagcggcc aggcggctcg ccgccggcgg tctgccgccg ccggtagcgc
     4861 gcctgctcgc ctggttctac agtgcggtgg cgagcccttt catcgacttc gtgctgcgct
     4921 atcgctggca ggcgctgctg ctgctggcgc tgatcgccac ctaccgcgtc tccgacatcg
     4981 tgctgggcgt gatgtccaac ccgttctacc gcgacatggg cttcaccaag gacgaggtgg
     5041 cgacggtgtc gggcgtgtac ggcgtgctga tgacgctggc gggctcggcg ctcggcggcg
     5101 tgctgtcgct gcggttcggg gtggtgcgcg tgctgttcct gggtgcggtg ctgtcggccg
     5161 ccaccaacct gatgttcgcg tggctcgcca cccgcggtca cgatttgccc ggcctggtga
     5221 tggtgatctc ggccgacaac ctgagcgccg gcatcgccag cgccgccttc gtggcctatc
     5281 tttccgggct caccaacgtc gcgtactccg ccacgcagta cgcgctgttc agctcggtga
     5341 tgcttttgct gccgaagttc atcgccggct ggtccggcat ggcggtggac ggcttcggct
     5401 accccgcctt cttcaccggc acggctttgc tcgggatgcc ggtgctgctg ctcacctggc
     5461 tggtgtggcg ggaatcgaag cgcgcgcagc cggcagcccc gtcaacgacc ccggcctgaa
     5521 caggcgtcta gcccggatat ttcaccaggc cacccccgcg tcgcatgcca ttgcgcacca
     5581 gcaggcgttc tcggagcatc cgttctgcgg gcacactttg gcttcggggt ttacgcggat
     5641 tctggcccgt tctggacggc catctcgggc atgcccgcct tgcc
//
LOCUS       DASPGE010000250         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1353989, whole genome shotgun sequence.
ACCESSION   DASPGE010000250 DASPGE010000000
VERSION     DASPGE010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353989"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VD791_08075"
     CDS             <1..580
                     /locus_tag="VD791_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HXV07972.1"
                     /translation="MRLLAMPLYHGKPLYQSYLIVPRADVVTRGYGDLEGKLFAYSDP
                     NSNSGFLVPQYLMLRAGLDPRSLFRKTFFTYAHRKVVSAVASGVADGGAVDGYVWETL
                     ALLSPEVTGKTRVAMKSPEFGFPPLVARASIGQGDYAAMQNVLVDMNADPEGRALLKR
                     LNLDGFARDDPKTFDGIAQALAYVSARMRLPQ"
     gene            619..2199
                     /locus_tag="VD791_08080"
     CDS             619..2199
                     /locus_tag="VD791_08080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXV07973.1"
                     /translation="MSDFLPDSPALVAGESERMPAAWRLPRLTGAQLPFRDLSYRFKI
                     PFSVSVVILVTVLVVSAVVVARSYHYLRADLIASAESLGKTLSRALTPALLRDEMWQA
                     YETIITPFDAADELESDRKTIVVLDASQQVYVSSHPRVFQSLTPLAAVSANHGELAAI
                     VARSASEAPFVVEDVLPNQIVVAVPVLSDDGIRLGTLLLTYAESLFLPRFWETMRQLL
                     LSTLMVLAVLIPLGWYAGNRMVIPLMGLARAMRRVSSEPPASVGADLYLGGDEIGQLG
                     RQFRAMLHELADKQALEREMIMSDRLASIGRLTAGIAHEINNPLGGMLNAINTCRRHG
                     SADPVTARTVSLLERGLLQIKETVAALLVEARVESHALTREDLEDVHTLVAHAAAKKQ
                     LAVRWYNWVEGTVPLPSTLVRQMLINLLLNAIQAAPARGLVACDVRVVPRAQDEGAAL
                     RLRVSNDGRHLEPGQLARLFEPFASETGTGLGLWVVYQIVQQLHGEIRVSNGPPITRF
                     ELSFPLKGEDTCPPQRNA"
BASE COUNT          363 a          789 c          817 g          395 t
ORIGIN      
        1 catgcggctg ctcgccatgc cgctgtacca cggcaagccg ctctaccaat cctatctgat
       61 cgtcccgcgc gcggacgtcg tcacccgcgg ttacggcgat ctcgagggca agctgttcgc
      121 ctactcggat ccgaactcca actcgggttt cctggtgccg cagtacctga tgctgcgtgc
      181 cgggctcgat ccgcgctcgc tgttccgcaa gaccttcttc acgtacgcgc accgcaaggt
      241 ggtgagcgcc gtggccagcg gcgtggccga cggcggcgcg gtcgacggct atgtgtggga
      301 gacgctggcg ttgctgtccc ccgaggtgac cggcaagacg cgggtggcga tgaagtcgcc
      361 agagttcgga tttccgccgc tggttgcgcg cgcctcgatc ggccagggcg actacgcggc
      421 gatgcagaac gtgctggtgg acatgaacgc cgacccggag gggcgcgctc tgctcaagcg
      481 cctcaatctc gatgggttcg cgcgcgacga cccgaagacc ttcgacggga tcgcgcaggc
      541 gctcgcttat gtctccgcgc gcatgaggct gccccagtag gggcgctgca ccggcacagg
      601 atcggagaaa ctcgagcgat gtccgatttc cttcccgaca gtccggcgct ggtcgccggc
      661 gaatcggagc gcatgccggc cgcctggcgg ctgccccggc tgaccggtgc gcagcttccg
      721 tttcgggact tgagctatcg cttcaagatc ccgttcagcg tgagcgtggt gatcctggtg
      781 accgtgctcg tcgtatcggc ggtggtggtc gcgcgctcct accactactt gcgcgccgac
      841 ctgatcgcca gcgccgagag cctgggtaag acgctgtcgc gcgcgctgac accggcgctg
      901 ttgcgcgacg agatgtggca ggcctacgag accatcatca cgccgttcga cgcagccgac
      961 gagctggaat ccgaccgcaa gacgatcgtc gtgctggatg cgagccagca ggtgtatgtg
     1021 tcgtcgcacc cgcgcgtgtt ccagtcgttg acgccgctcg ccgcggtgag cgccaaccat
     1081 ggtgaactcg ccgcgatcgt ggcgcgatcc gcctccgagg cgccgttcgt cgtggaggac
     1141 gtgttgccga accagatcgt ggtcgccgta ccggtgctgt ccgacgacgg catccgtctc
     1201 ggcacattgt tgctgaccta tgccgaatcg ctattcctgc cgcgcttctg ggagacgatg
     1261 cgccagcttc tgctgtccac actgatggtg ctggccgtcc tcattccgct gggctggtac
     1321 gccggcaacc gcatggtgat cccgctgatg gggctggcgc gcgccatgcg ccgggtgagc
     1381 agcgagccgc cggcgagcgt cggcgccgac ctgtacctgg gcggcgacga gatcggtcag
     1441 ctcgggcgcc agttcagggc gatgctccac gagttggcgg acaaacaggc gctggagcgg
     1501 gagatgatca tgtccgaccg cctggcgagc atcgggcggc tcaccgccgg catcgcccac
     1561 gagatcaaca accctctggg cggcatgctc aacgccatca acacctgtcg ccggcacggc
     1621 agcgccgacc ccgtgaccgc gcgcaccgtc tctctgctcg aacgcggcct gctgcagatc
     1681 aaggagacgg tggccgcgct gctggtggag gcgcgggtcg agagccatgc gctcacgcgc
     1741 gaggacctgg aggacgtgca cacgctggtg gcgcacgcgg ccgccaagaa gcagcttgcg
     1801 gtgcgctggt acaactgggt ggagggcacc gtgccgctgc cgtccacgct ggtgcgccag
     1861 atgctcatca acctgctgct caacgccatc caggcggcgc cggcgcgcgg cctggtcgcc
     1921 tgcgacgtgc gcgttgtccc cagggcgcag gacgaggggg cggcgctgcg gctgcgggtg
     1981 tccaacgacg gccgccatct cgaacccgga cagctcgcgc ggctgttcga gcccttcgcc
     2041 agcgagaccg gcaccgggct ggggctttgg gtcgtatacc agatcgtcca gcagttgcac
     2101 ggcgagatcc gcgtgagcaa cggtccgccg atcacgcgct tcgagctgag ctttccgctg
     2161 aagggcgagg acacgtgccc gccgcaacgc aacgcatgaa acccgtcgag agggaaaaga
     2221 tgagcacgct gccgaggctg tgcttgatcg aggacgacgt gctgatgggc gagtcgctgt
     2281 gcgatcgctt cgcgctcgaa ggcatcgcct tcgactggca ccgggacggc gccagcgccc
     2341 tgcgcagcat cgagcgcgag ccct
//
LOCUS       DASPGE010000251         4183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1706637, whole genome shotgun sequence.
ACCESSION   DASPGE010000251 DASPGE010000000
VERSION     DASPGE010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706637"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VD791_08085"
     CDS             <1..466
                     /locus_tag="VD791_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXV07974.1"
                     /translation="QHFRAPLRFDRDHSAVRFAAHWQRRPISGADPERRRTLTAAFAT
                     SPANDLVTQPHRALRLLLLSGKSGGDELAQALSLHRRTLSRRLRAEGTTFRSVLDEVR
                     FDVATQLLEHTRTPISEVATALCYAEVSAFMHAFRRWSGTTPARWRRAAVGQ"
     gene            570..1505
                     /locus_tag="VD791_08090"
     CDS             570..1505
                     /locus_tag="VD791_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV07975.1"
                     /translation="MTPLTDTPESAAVWARAGALVLAASALVLALIAAAPCPAAGPQW
                     PAIADPPTSQHVPGKWVWAELFTEDADAAVQFYGKVFGWSFQAFRAGRGPGYRLAFSD
                     GEPVGGMLEREHTREKAPGSRWLGMISVADVKAAARYAAEHGGKVVVPPRVLPGRGEV
                     ALLEDPEGAAFGVIRSSSGDPADYLAAERQWVWVELWARDPQAMAQFYSGLAGYEIAT
                     VERPDGSSGYLLASGGYTRCGIIASPAPKVASAWLPYLRVENVQAVAAQAEQAGARLL
                     VRPAPEIREGRVALILDPTGAAVGLARVTPQEVQQ"
     gene            1502..1816
                     /locus_tag="VD791_08095"
     CDS             1502..1816
                     /locus_tag="VD791_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07976.1"
                     /translation="MSRGVARSWRNALALLACTVAGGCAGDGSGGGSAGYSYGMHYGG
                     MWGYPPYYYDGPIYVGPPDTTPPPSGGGDRPGAGAPKPSHPIARPMPAPRPTPRGGGG
                     RR"
     gene            1875..2888
                     /gene="glsA"
                     /locus_tag="VD791_08100"
     CDS             1875..2888
                     /gene="glsA"
                     /locus_tag="VD791_08100"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF009020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HXV07977.1"
                     /translation="MNRVLPVAVVVVFAVLPPAAAAQGIPSAQVLGRAVQEAYEKFKD
                     VKDGANADYIPELAKVPSELFGVAIVTARGDVYTAGDVDYAFSIQSVSKPFTAGLVMQ
                     EQGSADVIREKIGVEPTGMKFNSLLATQVVKGVSVNPLVNSGAIASVSLVKAKTAEER
                     FDKIVKFYERLAGAKLSVIEDVYRSEAATNQRNRAHAYILFVSDRVYSDPMEAVDVYT
                     RQCSIGVTAKQLAVMGATLANNGVNPVTNQRVLDTKYVPKVLAIMMTAGFYDESGLWA
                     YTAGLPAKTGVGGGIVAVVPGRMAIVGFSPRVNEAGNSVRAMKAIEYISDQLGVNIFG
                     SGR"
     gene            complement(2885..3451)
                     /locus_tag="VD791_08105"
     CDS             complement(2885..3451)
                     /locus_tag="VD791_08105"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXV07978.1"
                     /translation="MLTAIHDLFNHPGLRAAFYKVRVPLALIAAAGVIWFAQPRWLWP
                     GFAVSMFGELIQLWSFASLNKDRDLACNGPYAVVRNPMYLGRFLILLGALMLVGSVWL
                     LLLYAVVYWFYMVNRVGREEARLRDALGAPYADYCAQVRRFLPGPPYRDNPLLYWNWG
                     LLRQNHGWTNLAGTLAFWVAAFLWMRFG"
     gene            complement(3473..>4183)
                     /locus_tag="VD791_08110"
     CDS             complement(3473..>4183)
                     /locus_tag="VD791_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07979.1"
                     /translation="PADASVTLANSRRTAPPPFTREQFERARGEVLSRGRWANAVLFL
                     HRQGDRLWVVKDFRPRNFLLRNSAGRLLVRREVRALQRLAGVAGVPAGVFRLDAHALA
                     YHFVPGSTLNQTDLGTRAVEFFLALERLMRQVHAVGGILHLDVRNARNVLVSERGEPL
                     LLDFQSHLSTRWMPQRMRRWAEGFDLAGTYKHWARRSPETLDADRARVLARMNRWRRL
                     WPLRGYLGVRKSRPSRAR"
BASE COUNT          659 a         1430 c         1496 g          598 t
ORIGIN      
        1 gcagcacttc cgggcgccgc tgcgcttcga ccgcgaccat tccgccgtgc gtttcgctgc
       61 gcactggcag cggcggccga tatcgggggc cgaccccgag cgccgccgca ctttgaccgc
      121 ggcgttcgcg acgtcgcccg ccaacgacct ggtcacacaa ccgcatcgcg cccttcgcct
      181 cctgctgctt tccggcaaga gcggcggcga cgagctggcg caagccctgt cgctgcaccg
      241 gcgcacgctg agccggcggc tgcgggccga gggcaccacc ttccggtcag tgctggacga
      301 ggtgcgcttc gacgtcgcga cccagctact cgagcacacg cgcaccccga tcagcgaagt
      361 cgccactgcg ctgtgctatg ccgaggtgag cgccttcatg cacgccttcc gtcgctggag
      421 cggcacgact cccgcccgat ggcgcagagc ggcggtcggc cagtagacgc aggcgcgatg
      481 tccagtattg tcaatccggc acccggaccg cccaggcaga atgtcatcct ccgatgatct
      541 cgaggacccc cggtggccga tcgcatccga tgactccgtt gacagacacc cctgaatcgg
      601 cggccgtctg ggcgcgggca ggcgccctcg tcctcgccgc cagcgcgctt gtgctcgccc
      661 tgatcgccgc cgcgccatgc ccggcggccg gtccacagtg gcccgcgatc gccgacccac
      721 cgacctcgca gcacgtcccc ggcaagtggg tgtgggcgga gctgtttacc gaggacgccg
      781 atgccgcggt gcagttctac ggcaaggttt tcggctggtc gttccaggcg ttccgcgcgg
      841 ggcgggggcc cggctaccgg ctggcgttct ccgacggtga gccggtcggc ggcatgctcg
      901 agcgcgagca tacgcgcgag aaggcgcccg gcagccgctg gctgggcatg atctcggtgg
      961 ccgatgtgaa ggcggcggcg cgctacgccg ccgagcacgg cggcaaggtg gtcgtgccgc
     1021 cgcgcgttct gccgggccgc ggcgaggtgg cgctgctcga ggatcccgaa ggcgccgcgt
     1081 tcggcgtgat ccgctcgtcc agcggagacc cggccgacta cctggccgcc gagaggcagt
     1141 gggtctgggt cgaactgtgg gccagggatc cgcaggcgat ggcgcagttc tacagcgggc
     1201 tggccggtta cgagatcgcg accgtcgagc gcccggacgg cagctccggt tatctgctcg
     1261 cctcgggcgg ttacacgcgc tgcggcatca tcgcatcgcc cgcgcccaag gtcgcatcgg
     1321 catggctgcc ttacctgcgg gtagaaaacg tccaggctgt ggcggcccag gccgagcagg
     1381 ccggcgcccg gctgctcgtc cggcctgctc ccgagatccg tgaaggccgc gtggcgttga
     1441 tcctcgatcc cacgggcgca gcggtcggcc tcgcgcgcgt gacgccgcag gaggtgcagc
     1501 aatgagccgc ggcgtcgcac ggtcgtggcg caacgcgctc gcgctgctcg cttgcacggt
     1561 cgcaggcggt tgtgcaggag acggcagcgg cggcggcagc gccggctaca gctacggcat
     1621 gcactacggc ggcatgtggg gatatccgcc ctattactac gacggtccga tctacgtcgg
     1681 cccgccggac accacgccgc cgccttccgg tggcggcgat cggcccggtg caggtgcacc
     1741 gaagccctcg catccgatcg cacgcccgat gccggcgccg cgtccgacac cgcgcggggg
     1801 cggcgggcgt aggtagtttc atgcagacag cgagctcgac cagcgtcgtg tccggtaggc
     1861 ggctggaagg cgtgttgaat cgggtcctcc cggtggccgt ggtggtcgtc ttcgccgttc
     1921 tgccgccggc ggccgcggcg cagggcattc cgagcgcgca ggtgctcggc cgcgccgtgc
     1981 aggaggcgta cgagaagttc aaggacgtca aggacggagc gaatgccgac tacattccgg
     2041 agctggccaa agtgccctcg gagctgttcg gcgtggcgat cgtcaccgcg cgcggcgacg
     2101 tctacacggc cggcgacgtg gactatgcgt tcagcatcca gtcggtgtcc aagccgttca
     2161 ccgccgggct ggtgatgcag gagcagggca gcgccgacgt gatccgcgag aagatcgggg
     2221 tggagcccac tggcatgaag ttcaactcgc tgctcgccac gcaggtggtc aagggcgtgt
     2281 cggtgaatcc gctggtgaac tccggcgcga tcgcctcggt cagcctggtc aaggccaaga
     2341 ccgcagagga gcgcttcgac aagatcgtca agttctacga gcgtctggcc ggggccaagc
     2401 tgagcgtcat cgaggacgtg tatcggtccg aagcggccac caaccagcgc aaccgcgcgc
     2461 acgcctacat cctgttcgtg tccgaccgcg tctattccga cccgatggaa gcggtggacg
     2521 tgtacacgcg gcagtgctcg atcggcgtca ccgccaagca gctggcggtg atgggtgcga
     2581 cgctggccaa caacggcgtc aacccggtca cgaaccaacg cgtactggac acgaagtacg
     2641 tgccgaaggt gctcgccatc atgatgaccg cgggcttcta cgacgaatcg ggactgtggg
     2701 cgtataccgc gggactgccc gccaagaccg gcgtgggcgg cgggatcgtc gccgtggtgc
     2761 cgggcaggat ggcgatcgtc ggcttctccc cgcgcgtgaa cgaggcgggc aacagcgtgc
     2821 gcgcgatgaa ggccatcgag tacatcagcg accagctcgg ggtgaacatt ttcggcagcg
     2881 ggcgctagcc gaacctcatc cacaggaacg ccgcgaccca gaacgccagg gtgccggcga
     2941 ggttggtcca gccgtgattc tggcgcagca ggccccagtt ccagtacagc agcgggttgt
     3001 cgcggtacgg cggaccgggc aggaagcggc gcacttgcgc gcagtagtcc gcgtacggtg
     3061 ctcccagcgc gtcgcgcagg cgcgcttcct cgcgcccgac ccggttgacc atgtagaacc
     3121 agtacaccac cgcgtagagc agcagcagcc agacgctgcc gacgagcatc agcgcgccca
     3181 gcaggatcag gaagcggccg aggtacatgg gattgcgcac cacggcgtag gggccgttgc
     3241 acgccaggtc gcggtccttg ttgagcgagg cgaacgacca cagctggatc agctcgccga
     3301 acatcgacac cgcgaagccc ggccacagcc agcgcggctg cgcgaaccag atgacgccgg
     3361 cggccgcgat cagcgcgagc ggtacgcgga ccttgtagaa cgccgcgcgc aggccgggat
     3421 gattgaagag gtcgtggatg gcggtcagca tgatcggccc gggttcgaat gatcagcgag
     3481 cgcgcgatgg tcgggatttg cgcacgccga ggtagccgcg cagcggccaa agccgccgcc
     3541 agcggttcat ccgcgcgagc accctggcgc ggtccgcgtc gagggtctcc gggctgcggc
     3601 gcgcccagtg cttgtaagtg ccggcgagat cgaagccctc cgcccagcgg cgcatgcgct
     3661 gcggcatcca gcgcgtgctc aggtgcgact ggaagtcgag cagcagcggc tcgccgcgct
     3721 ccgacaccag cacgttgcgc gcgttgcgca cgtccaggtg caggatcccg cccaccgcgt
     3781 gcacctgccg catcagccgc tcgagcgcga ggaagaactc cacggcgcgt gtgccgagat
     3841 cggtctgatt gagcgtggag cccgggacga aatgataggc cagcgcgtgc gcatcgagcc
     3901 ggaacacgcc ggccggcacg ccggcgaccc ccgcgagccg ctgcagcgcg cgcacctcgc
     3961 gccgcaccag caagcggccc gcgctgttgc gcagcagaaa gttgcgcggg cggaagtcct
     4021 tcaccaccca cagccgatcg ccctggcgat gcaggaacag cacggcgttg gcccagcggc
     4081 cgcggctgag cacctcgccg cgcgcacgct cgaattgctc ccgggtgaag ggcgggggcg
     4141 cggtccggcg ggaattcgcg agtgtcaccg aggcgtcggc tgg
//
LOCUS       DASPGE010000252         3614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_302760, whole genome shotgun sequence.
ACCESSION   DASPGE010000252 DASPGE010000000
VERSION     DASPGE010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302760"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1906)
                     /locus_tag="VD791_08115"
     CDS             complement(35..1906)
                     /locus_tag="VD791_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXV07980.1"
                     /translation="MTNLWTRPRLPGQRLGLPGALSGETVPELFWNAVRQHGQRVALR
                     QKDFGIWQVTTWRELGEIARDIAMGLAALGFEPGECASILSNTRREWMFADLGILSAG
                     GVSNGIYPTDAAGQCEYLLNDSSSAYVFVEDEEQLDKVLEIRARTPTLRRIVVFDMEG
                     LARFSDPQVMSLEALRELGRAHAGAHPEELERRIAARKPADLAILVYTSGTTGRPKGA
                     MISHHNLVHVVRGYDAVLPRANAIDERMCFLPLCHIAERVGGEYLALLSGGVLNFVEN
                     PDTVPENVREISPTVVTAVPRVWEKFYSAVTIAVKEATVLQQLAYRWAIGVGGRVADR
                     FVAGRPVPAWLKLQFLAARWLALDNVRKLIGIHRARFLVTGAAPISPDLVRWYLALGI
                     PMLEAWGQTESSGAATFMPVDRIKPGSIGRAGPYNEVRVSAEGELLIRGDNVFMGYLN
                     QPEKTAETLREGWLHTGDVGAVDEEGFFRITDRMKDIIITAGGKNVTPSEIENQLKFS
                     PYITDAVVIGDRRAFLTALIMIDQENVERYAQDHDIPFSNYASLCRAREIQELIRGEV
                     ERVNRQFARVEQIKSFRLIEQRLTAEDEELTPTMKLKRKLVHQKYAELIESMYSRAG"
     gene            complement(1908..2681)
                     /locus_tag="VD791_08120"
     CDS             complement(1908..2681)
                     /locus_tag="VD791_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV07981.1"
                     /translation="MSEPILQLANVESAYGPVKAIRGVSLSVASGSIVTVLGANGAGK
                     STILKTISGILDPQKGSIVYRGAAIHGKDPAQIVRLGISHVPEGREVFPLLSVYDNLM
                     MGAYTRRDRDGVAQDLQTVYGYFPMLRARARQEAGQLSGGEQQMLAISRALMSRPQIM
                     LLDEPSLGLSPRLTREIFAIIQRINRERGTTVLLVEQNAALALDLADYGYVLEVGRIV
                     MEDDCARLREKEDIREFYLGMKEAGVRGERRWKKKKMWR"
     gene            complement(2678..3451)
                     /locus_tag="VD791_08125"
     CDS             complement(2678..3451)
                     /locus_tag="VD791_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV07982.1"
                     /translation="MSAALLQVDRLSVQFGGVKAVSGVGFTVRQGEVLTLIGPNGAGK
                     TTVFNLISRIYQPASGAIALDGVDLLRVPPHRVAALGIARTFQNIELFENATVLQNLL
                     IGCHTHRATGLWQELLFLPAVRREERKLRDRVEQVIDLLDLQHHRDSLVAGLPYGVRK
                     VVELGRALAARPRLLLLDEPSSGLNVEETDDMAFWIQDIRDDLGVTVLMVEHDMALVS
                     RVSDRVLAMNQGEVIAQGTPAEVQAHPAVIEAYLGKAAA"
     gene            complement(3448..>3614)
                     /locus_tag="VD791_08130"
     CDS             complement(3448..>3614)
                     /locus_tag="VD791_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV07983.1"
                     /translation="PTVFGLILIGFILFEPMGLYGWWFKARTYFSLFPFYRRGTFKRR
                     KAFQKSERLR"
BASE COUNT          559 a         1275 c         1202 g          578 t
ORIGIN      
        1 cgctgcggga gagggggcgg gggtgagggg gagatcatcc agcgcgactg tacatggatt
       61 cgatcagctc ggcgtacttc tggtgcacca gcttgcgctt gagcttcatg gtcggggtga
      121 gctcctcgtc ctcggcggtg aggcgctgct cgatgagccg gaagctcttg atctgctcca
      181 cccgggcgaa ctggcgattc acgcgctcga cctcgccgcg gatcagctcc tggatctcgc
      241 gcgcccggca caggctggcg tagttcgaga acgggatgtc gtggtcctgg gcgtagcgct
      301 cgacgttttc ctgatcgatc atgatcagcg ccgtgaggaa ggcacgccgg tcgccgatca
      361 ccacggcgtc ggtgatgtag ggcgagaact tgagctggtt ctcgatctcg gagggagtga
      421 cgttcttgcc gcccgcggtg atgatgatgt ctttcatgcg gtcggtgatg cggaagaagc
      481 cctcctcgtc caccgcgccc acgtcgccgg tgtgcagcca gccctcgcgc agcgtctcgg
      541 ccgtcttctc gggctggttc aagtagccca tgaacacgtt gtccccgcgg atcagcagct
      601 cgccttcggc ggacacgcgc acctcgttgt aggggccggc acgcccgatc gagccgggct
      661 tgatgcggtc caccggcatg aaggtggccg cgcccgagct ttcggtctgc ccccacgcct
      721 ccagcatcgg gatgcccagc gccaggtacc agcgcaccag gtcgggggag atcggcgccg
      781 cgccggtcac caggaagcgg gcgcgatgga tgccgattag cttgcgcacg ttgtcgagcg
      841 cgagccagcg cgccgccagg aactgcagct tcagccatgc ggggaccggc cgcccggcga
      901 cgaagcggtc ggcgacccgg ccgccgaccc cgatcgccca gcggtaggcg agctgctgca
      961 ggacggtggc ctccttcacg gcgatggtca cggcggagta gaacttctcc cacacgcgcg
     1021 ggacggcggt gacgacggtg ggcgagatct cgcgcacgtt ctccggcacg gtatcggggt
     1081 tctccacgaa gttgagcacg ccgccgctca gcagcgccag gtactccccg cccacccgct
     1141 cggcgatgtg gcacagcggc aggaagcaca tccgctcgtc gatcgcgttg gcgcgcggca
     1201 ggaccgcgtc gtagccgcgc accacgtgca ccaggttgtg gtgcgagatc atggcgccct
     1261 tgggcctgcc ggtggtaccc gaggtgtaca ccaggatggc gaggtcggcc ggcttgcgcg
     1321 cggcgatgcg tcgctccagc tcctcgggat gcgcgcctgc gtgggcgcgc cccagctcgc
     1381 gcagcgcctc caggctcatc acctgcgggt ccgagaagcg cgccagacct tccatgtcga
     1441 acacgacgat gcgccgcagc gtcggcgtgc gcgcacggat ctcgagcacc ttgtcgagct
     1501 gctcctcgtc ctccacgaac acgtacgccg aggacgagtc gttcagcagg tactcgcact
     1561 gcccggccgc atcggtggga tagatgccat tggacacccc gccggcggac aggatgccga
     1621 ggtcggcgaa catccattcg cggcgcgtgt tggacaggat cgaggcgcac tcccccggct
     1681 cgaagccgag cgccgccaga cccatggcga tgtcgcgcgc gatctccccc agctcgcgcc
     1741 aggtcgtgac ctgccagatg ccgaaatcct tctgccgcag cgccacgcgc tggccgtgct
     1801 ggcgcacggc gttccagaac agctcgggga cggtttcgcc ggacagcgcc ccgggcagcc
     1861 cgaggcgctg cccgggcagg cgcgggcggg tccagaggtt ggtcatgcta cctccacatc
     1921 ttcttcttct tccaccgccg ctctccgcgc acgcccgctt ccttcatccc caggtagaac
     1981 tcgcgaatgt cctctttctc ccgcagccgg gcgcagtcgt cctccatgac gatgcggccg
     2041 acctcgagca cgtagccgta gtcggccaga tccagcgcca gggcggcatt ctgctcgacc
     2101 agcaggacgg tagtcccgcg ctcgcggttg atgcgctgga tgatagcgaa gatctcccgc
     2161 gtgagcctgg gcgacagtcc gaggctgggc tcgtcgagca gcatgatctg cggacggctc
     2221 atcagcgcgc ggctgatggc cagcatctgc tgctcgccgc cggagagctg gcccgcctcc
     2281 tggcgcgcgc gcgcgcgcag catcgggaag tagccgtaca ccgtctgcag gtcctgcgcc
     2341 acgccgtcgc ggtcgcgccg cgtgtacgcc cccatcatca ggttgtcgta taccgacagc
     2401 agcgggaaca cctcgcggcc ctccggcacg tgcgagatgc ccaggcgcac gatctgcgcc
     2461 gggtccttgc cgtggatggc cgcgccgcga tagacgatcg accccttctg cgggtccagg
     2521 atcccggaga tggtcttcag gatcgtcgac ttgcccgcgc cgttggcgcc cagcaccgtg
     2581 acgatcgacc cgctcgccac cgacaggctc acgccgcgga tcgccttcac cggcccgtag
     2641 gcgctctcga cgttggcgag ctgcaggatc ggttcgctca tgccgccgcc ttacccagat
     2701 acgcctcaat caccgccgga tgcgcctgca cctcggccgg cgtgccctgg gcgatcacct
     2761 cgccctggtt catcgccagc acgcggtccg acacgcgcga caccagcgcc atgtcgtgct
     2821 ccaccatcag cacggtgacg ccgaggtcgt cgcggatgtc ctggatccag aacgccatgt
     2881 cgtcggtctc ctccacgttc aacccggaag acggctcgtc gagcagcagc aggcgcggcc
     2941 gcgccgccag cgcgcggccc agctccacca ccttgcgcac cccgtagggc aggcccgcga
     3001 ccaggctatc gcggtggtgc tgcagatcga gcaggtcgat cacctgctcc acccgatcgc
     3061 gcagcttgcg ctcttcccgc cgtaccgcgg gcaggaacag cagctcctgc cacaggccgg
     3121 tggcgcgatg cgtgtggcag ccgatgagca ggttctgcag cacggtggcg ttctcgaaca
     3181 gctcgatgtt ctggaacgtg cgcgcgatgc cgagcgcggc gacgcgatgc ggcggcacgc
     3241 gcagcagatc gacgccgtcg agcgcgatcg cgccggaggc gggctggtag atgcggctga
     3301 tcaggttgaa caccgtggtc ttgcctgcgc cgttggggcc gatcagcgtg agcacctcgc
     3361 cctgccgcac cgtgaagccg acgcccgaca ccgccttgac gccaccgaac tgcacgctca
     3421 gacggtccac ctgcaacagc gccgcgctca tcgcagccgc tccgatttct ggaacgcctt
     3481 gcgccgctta aacgtgccgc gccggtagaa cgggaacagc gagaaatagg tgcgcgcctt
     3541 gaaccaccag ccgtacagcc ccatcggctc gaacaggatg aagccgatca ggatcagccc
     3601 gaacacggtg ggct
//
LOCUS       DASPGE010000253         6399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_418662, whole genome shotgun sequence.
ACCESSION   DASPGE010000253 DASPGE010000000
VERSION     DASPGE010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418662"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VD791_08135"
     CDS             complement(<1..378)
                     /locus_tag="VD791_08135"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802447.1"
                     /note="FabH; beta-ketoacyl-acyl carrier protein synthase
                     III; catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HXV07984.1"
                     /translation="MTMYSGIVGTGSYLPEKVLTNRDLERMVDTSDEWIVSRTGIRQR
                     HVAGEGELASDLALRAAERALKSAGISAQELGLIVVATTTPDMVFPSTACILQAKLGV
                     QQAPAFDVQAVCSGFIYALGIADM"
     gene            complement(375..1409)
                     /gene="plsX"
                     /locus_tag="VD791_08140"
     CDS             complement(375..1409)
                     /gene="plsX"
                     /locus_tag="VD791_08140"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HXV07985.1"
                     /translation="MRITIALDCMGGDHGPEVTVPAAAAFLQQRPDAHVILVGRRDEI
                     EARLRGLHLAGDARLRVQHAPEVVDMDEPPASALRAKRDSSMRLAVDLVKSGEADACV
                     SAGNTGALMAISRFVLKMLPGVERPAIASMVPTMRGRSCVLDLGANVDCTPLNLMQFA
                     VMGAALYSVVEKAERPSVGLLNIGEEDIKGNEVAKQAGELLRASDLNFHGNIEGYDVY
                     KGTTDVVVCDGFVGNVFLKSSEGLARMLAAYLHEEFTRNAFTKLLGFLCLPVINAFRR
                     RVDPRRLNGASLLGLRGVVVKSHGSADSFAFAVAINRAWEEAESGILERIMAHLPHVD
                     QPGEQVQKAG"
     gene            complement(1507..1686)
                     /gene="rpmF"
                     /locus_tag="VD791_08145"
     CDS             complement(1507..1686)
                     /gene="rpmF"
                     /locus_tag="VD791_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HXV07986.1"
                     /translation="MAVQQNKKSASRRNMRRSHDALGAPPTAIEPVSGEVHLRHHISP
                     NGYYRGKKVVQSRED"
     gene            complement(1714..2229)
                     /locus_tag="VD791_08150"
     CDS             complement(1714..2229)
                     /locus_tag="VD791_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HXV07987.1"
                     /translation="MSEQLVIDPARFAREGARLTGALAAAALPRLFDQLQRADQLQGA
                     GGEIGYQVDGFLSERGHPGLHLQLNGEIELRCQRCLGALRARIESRRDVVLVPDADEF
                     AQRSDEDETMDIIPAVPRLDLRTLLEDEVLLALPVAPCHAPDECRVPEGRPGAAPERV
                     SPFAALAQLKR"
     gene            2324..2896
                     /locus_tag="VD791_08155"
     CDS             2324..2896
                     /locus_tag="VD791_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HXV07988.1"
                     /translation="MLVLASTSAYRRELLARLRLPFEVAAPHVDETPHAGELPAQTAL
                     RLAQEKARAVAARFAQALVIGSDQVAELEGERLDKPGGHERARAQLQLLSGRAAVFHT
                     AVALLNSGTGRMQARVVTTRVRFRRLDAAQIESYLLAEQPYDCAGAAKSEGLGIALIE
                     AIQGDDPTALIGLPLIALTDMLTAEGHPLV"
     gene            2970..3662
                     /locus_tag="VD791_08160"
     CDS             2970..3662
                     /locus_tag="VD791_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXV07989.1"
                     /translation="MYLIPTPLGPAPVDASLPPQARALACSLDGFVAESAKSARRFLK
                     AIGHPGPLSGISIAELNEHTPASALPALLAPAQAGRRIGLLSEAGCPGVADPGATLVA
                     LAHAQGVPVVPLVGPSSLLLALMASGLNGQQFCFHGYLPVEGSALEAAIRALEAQSLR
                     ESSTQLFIEAPYRNNRLLQALLQVCRADTRLCLATDLTLATQSIATRRVADWCGAVPD
                     IDRRPTVFVLQG"
     gene            complement(3698..4723)
                     /locus_tag="VD791_08165"
     CDS             complement(3698..4723)
                     /locus_tag="VD791_08165"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HXV07990.1"
                     /translation="MTLSNVTPESSSTPDSSTPAGASAPPPGPPRAPTDERWARDALE
                     RLARAALDEQRRARHWGIFFKTLTFLYLFALLFIAIGWVGGREPIAGRHTALVALEGV
                     IAARSEASAERINQGLQDAFADGNSAGVIVRINSPGGSPVQAGQINQEIRRLRGLHPE
                     KPLYVVVEDICASGGYYVAAAADRIYVDPASIVGSIGVLMDGFGFTGTMDKLGVERRL
                     ITAGRNKGFLDPFSPMDEKERAYAQQMLDEIHQQFIDLVKAGRGERLKETPELFSGLV
                     WTGEKSIELGLADAVGSVEYVAREVIKAERVVDYTPAENLAERFIRRFGAAAAETLVR
                     LGAGALR"
     gene            complement(4720..5064)
                     /locus_tag="VD791_08170"
     CDS             complement(4720..5064)
                     /locus_tag="VD791_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HXV07991.1"
                     /translation="MAGSKQLICDADALTDGGRGVRFTVQRHGREEPAFAVRHDGAVR
                     AFLNRCGHIPAELDWSEGEFFDLSGLYLMCATHGALYLPDTGRCVGGPCAGRGLVPLA
                     VEESDGKVFLID"
     gene            complement(5043..5702)
                     /locus_tag="VD791_08175"
     CDS             complement(5043..5702)
                     /locus_tag="VD791_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXV07992.1"
                     /translation="MTARRFELLVFDWDGTVVDSAAHIVASIQAACADLGLAVPRADR
                     ARYIIGLGMQDAMQHLLPELPAADYPRLAERYRHHYLAGDEKVVPFGGAREGIARLHA
                     AGFLLGVATGKSRRGLDQALQNTGLQGFFHASRCADEGFAKPHPDMLLFLMERLNVAP
                     ARTLMIGDTTHDLEMARSAGVPALAVSYGAHAEAQLAGLAPLACVRSFEDLMAWLEAN
                     S"
     gene            complement(5699..>6399)
                     /locus_tag="VD791_08180"
     CDS             complement(5699..>6399)
                     /locus_tag="VD791_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXV07993.1"
                     /translation="APLLGRRPLLEDEWLLAIDKPAGVAVHGGSGVSRGAIEQLRLER
                     PALRFLELVHRLDRDTSGVLLLAKKRSALIELHRQLRDGLVEKRYLVLVAGRWRSARQ
                     VVKLPLVRRLTAQGERRVSVEAGGMPSHTEFTLQRHLPGFSLLAAQLRTGRTHQIRVH
                     LAHLGFPIAGDDKYGDFGLNKALVKRGLKRMFLHAAELGFRHPASGEQLRLTAPLPPD
                     LAGFLAALETERGR"
BASE COUNT          950 a         2326 c         2163 g          960 t
ORIGIN      
        1 catatcggcg atgccgagcg catagatgaa gccgctgcac accgcctgca catcgaacgc
       61 cggcgcctgc tgcaccccga gcttggcctg caggatgcag gcggtgctcg ggaacaccat
      121 gtccggggtg gtggtggcga ccacgatcag ccccagctcc tgcgccgaga tgccggcgct
      181 cttcagcgcg cgctcggccg cgcgcagcgc cagatcgctc gccagctcgc cctcgcccgc
      241 cacgtggcgc tggcggatgc cggtgcgcga cacgatccac tcgtccgagg tgtccaccat
      301 gcgttccagg tcgcggttgg tgagcacctt ctcgggaaga tagctgccgg tgccgacgat
      361 gccggagtac atggtcagcc ggctttctgc acctgctcgc cgggctggtc cacgtgcggc
      421 agatgcgcca tgatgcgctc caggatgccg ctctcggcct cctcccaggc gcggttgatg
      481 gccaccgcga aggcgaagct gtcggccgag ccgtggctct tcaccaccac gccgcgcagc
      541 cccagcaggc tggcgccgtt cagccggcgc gggtccaccc gccggcggaa ggcgttgatc
      601 accggcaggc acaggaagcc gagcagcttg gtgaacgcat tgcgcgtgaa ctcttcgtgc
      661 agataggccg ccaacatgcg cgccaggccc tcggaggact tcaggaacac gttgccgacg
      721 aagccgtcgc acaccaccac gtcggtggtg cccttgtaga cgtcgtagcc ctcgatgttg
      781 ccgtggaaat tgagatcgct cgcgcgcagc agctcgcccg cctgcttggc cacctcgttg
      841 cccttgatgt cctcctcgcc gatgttgagc agccccacgc tcgggcgctc ggctttctcc
      901 accacgctgt acagggccgc gcccatcacc gcgaactgca tcaggttgag cggcgtgcaa
      961 tcgacgttgg cgcccaagtc gagcacgcag gaacgtccac gcatggtcgg caccatcgag
     1021 gcgatcgccg ggcgctccac accgggcagc atcttcagca cgaagcgcga gatcgccatc
     1081 agcgcgccgg tgttgccggc gctgacgcag gcgtccgcct cgccggactt gaccaggtcc
     1141 accgccaggc gcatggacga atcgcgcttc gcgcgcaggg cggacgccgg aggctcgtcc
     1201 atgtcgacca cctccggcgc gtgctgcacg cgcagacgcg catcgcccgc gaggtgcagc
     1261 ccacgcaggc gcgcctcgat ctcgtcgcgg cggccgacca ggatgacgtg cgcatccggc
     1321 cgctgctgca ggaaggcggc cgctgccgga acggtcactt ccgggccgtg gtcaccaccc
     1381 atgcaatcga gggcgatcgt gatgcgcatc agggctgcgc ccccgtcatc ggcgggcgcc
     1441 ggcaagagag gcggccgcgc aggcgagacc gcgtcgcgaa gtgaggccga tcacgccgtc
     1501 gcgcaatcag tcttcgcggg actggacgac cttcttcccg cgatagtagc cgttcgggct
     1561 gatgtggtgg cgcagatgca cctcgccgga caccggctcg atcgccgtcg gcggcgcgcc
     1621 gagcgcgtcg tgggagcggc gcatgttccg ccgggatgct gacttcttgt tctgctgaac
     1681 ggccatcgtg tactcctgtg gaagctgtcc gaatcaacgt ttgagctgcg ccagcgcagc
     1741 gaagggcgac acgcgctcgg gcgcggcgcc gggccggcct tcgggcacgc ggcactcgtc
     1801 gggcgcgtgg cagggcgcca ccggcaaggc cagcagcacc tcgtcttcca gcagcgtgcg
     1861 caagtcgagc cgcggcaccg ccgggatgat gtccatcgtc tcgtcctcgt cgctgcgctg
     1921 ggcgaactcg tcggcatccg gcaccagcac gacgtcgcga cggctttcga tgcgcgcgcg
     1981 cagcgcgccc aggcagcgct ggcagcgcaa ttcgatctcg ccgttcagct gcaggtgcag
     2041 gccgggatgc ccgcgctcgc tcaggaagcc gtcgacctgg tagccgatct cgccgcccgc
     2101 tccctgcaac tgatcggccc gctgcaactg atcgaagagg cggggcagcg ccgcggccgc
     2161 aagcgcgcct gtcaatcgcg ccccttcgcg cgcgaagcgg gccggatcga tgacgagctg
     2221 ttccgacata aacggcgcat ggtagtattt ttgtgtcgca gtgtcaaaac cttaagatcg
     2281 aatcctcggg cccgcaccgg gcctgcaacg cgccctctcg ccgatgctgg ttctcgcctc
     2341 cacttccgcc taccggcgcg agctgctggc gcgcctgcgc ctgcctttcg aggtcgccgc
     2401 tccgcacgtg gacgagacgc ctcacgcggg cgagctgccc gcgcaaaccg cgctgcgcct
     2461 ggcgcaggag aaggcccgcg ccgtcgccgc gcgctttgcg caagcgctgg taatcgggtc
     2521 cgatcaggtc gccgagctcg aaggcgagcg cctggacaag cccggcggcc acgagcgcgc
     2581 gcgcgcgcag ctgcaactgc tgagcgggcg cgcggccgtg ttccacaccg ccgtcgccct
     2641 gctcaacagc ggcaccggcc gcatgcaggc gcgcgtagtg acgacccgcg tgcgcttccg
     2701 gcggctcgac gcggcgcaga tcgaatccta cctgctcgcg gagcagccct acgactgcgc
     2761 cggcgccgcg aaatccgaag gcctcggcat cgcgctgatc gaagcgatcc agggcgacga
     2821 ccccaccgca cttatcggcc tgccgctgat cgccttgacg gatatgctga ccgccgaggg
     2881 tcacccgctt gtctgaggac agttccgcgt tgtccgcgca gcgttcctcg gaaacctcgc
     2941 acatcgggga aacctcccga actggcaggt tgtacctgat ccccacgccg ctcggcccgg
     3001 ctcccgtgga cgcgagcctg cccccgcagg cgcgcgcgct ggcgtgcagc ctggatgggt
     3061 tcgttgccga gagcgcgaag agcgcgcggc ggtttctcaa agccatcggc caccccggcc
     3121 ctctgtccgg catctccatc gccgagctca acgagcatac gccggcctcg gcgctgccgg
     3181 cgctgctcgc gccggcgcag gcgggccggc gcatcggatt gctgtcggaa gcgggctgtc
     3241 cgggcgtggc cgacccgggg gcgaccctgg tcgccttggc gcacgcgcag ggcgtgcccg
     3301 tggtgcctct ggtaggcccc tcgtcattgc tgctggcgct gatggcctcc gggctcaacg
     3361 gccagcagtt ctgcttccac ggctacctcc cggtggaagg ctcggcgctc gaggcggcga
     3421 tccgggcgct cgaggcgcaa tcgctgcgcg agagcagcac ccagctgttc atcgaagccc
     3481 cctaccgcaa caaccggctg ctgcaggcgc tgctgcaagt gtgccgcgcc gacacgcgcc
     3541 tttgcttggc gacggacctg acgctcgcta cccagtcgat cgctacgcgg cgcgtggcgg
     3601 actggtgcgg cgccgtcccc gacatcgatc gccgtcccac ggtgttcgtt ctacaggggt
     3661 gaggaagtaa caatcccccg gctctgccgg ggaattgtta tcgcagcgcg ccggcgccca
     3721 ggcgcaccaa cgtctcggcc gccgcggcgc cgaagcggcg gatgaagcgc tcggcgaggt
     3781 tttccgccgg cgtgtagtcg accacgcgct cggccttgat cacctcgcgt gcgacgtatt
     3841 cgacgctgcc caccgcgtcg gccagcccca gctcgatgct cttctctccg gtccacacca
     3901 ggccgctgaa cagctcgggg gtctccttca agcgctcgcc gcggccggcc ttcaccaggt
     3961 cgatgaactg ctgatggatc tcgtccagca tctgctgcgc gtaggcgcgc tctttctcgt
     4021 ccatcgggga gaagggatcg aggaagccct tgttccggcc cgccgtgatc aggcgccgct
     4081 cgacgccgag cttgtccatc gtaccggtga agccgaaccc gtccatcagc acgccgatcg
     4141 atccgacgat gctggccggg tccacataga tgcgatcggc ggcggcggcc acgtagtagc
     4201 cgcccgaggc gcagatgtcc tccaccacga cgtacagcgg cttttcggga tgcagcccgc
     4261 gcagccgccg gatctcctgg ttgatctgac cggcctgcac cgggctgccg ccggggctgt
     4321 tgatgcgcac gatcaccccg gcgctgttgc cgtcggcgaa agcgtcctgg agcccctgat
     4381 tgatgcgctc cgcgctggct tcgctgcgcg cggcgatcac cccctccagc gccaccaagg
     4441 cggtgtgccg gcccgcgatc ggctctctgc cgccgaccca tccgatggcg atgaacagca
     4501 gcgcgaacag gtagaggaag gtgagagtct tgaagaagat gccccagtgg cgggcgcggc
     4561 gctgctcgtc gagggcggcg cgggccaagc gctcgagcgc atcgcgcgcc cagcgctcgt
     4621 cggtgggcgc gcgcgggggg ccgggcggcg gcgcggacgc gccggccggc gtgctggaat
     4681 ctggcgtact ggaactctct ggcgtcacgt tggagagcgt caatcgatca ggaacacctt
     4741 gccgtcggac tcttcgaccg ccagcggcac cagcccgcgc ccggcgcagg gaccgcccac
     4801 gcagcgcccg gtgtcgggca ggtacagcgc gccgtgtgtc gcgcacatca agtataaccc
     4861 gctcaggtcg aagaactccc cttcgctcca atcgagctcg gccggaatgt ggccgcagcg
     4921 gttgagaaac gcacgcaccg cgccgtcgtg ccgcacggcg aacgccggct cctcgcggcc
     4981 gtgacgctgg acggtgaagc gcacgccgcg gccgccgtcc gtcaaggcat cggcgtcgca
     5041 gatcagctgt ttgcttccag ccatgccatg aggtcttcga agctgcgcac gcaggcgagc
     5101 ggcgccaggc cggcgagctg tgcttcggcg tgtgcgccat agctcaccgc caaggccggc
     5161 acacccgcgc tgcgcgccat ctccagatcg tgcgtggtgt cgccgatcat taacgtccgc
     5221 gccggcgcca cattgagccg ttccatcagg aacagcagca tgtcgggatg cggcttggcg
     5281 aagccttcgt cggcgcagcg cgaggcatga aagaagccct gcaggccggt attctgcaac
     5341 gcctggtcga gcccgcgccg gctcttaccg gtggccacgc cgagtaggaa ccccgccgcg
     5401 tgcaggcgcg cgatgccctc gcgcgccccg ccgaacggga cgaccttctc gtcgccggcg
     5461 agataatggt gccggtagcg ctccgcgagc ctcgggtagt cggccgccgg cagctccggc
     5521 aggaggtgct gcatcgcgtc ctgcatcccc aggccgataa tgtagcgcgc ccggtccgcg
     5581 cgcggcacgg cgagccccag gtcggcgcag gccgcctgga tcgacgccac gatgtgcgcg
     5641 gccgaatcca ccacggtgcc atcccagtcg aacaccagca gctcgaagcg gcgcgcggtc
     5701 atcgcccgcg ctccgtttcc agcgccgcga gaaacccggc caggtcgggc ggcagcggcg
     5761 cggtcagccg cagctgctcg ccgctggcgg ggtgccggaa gccgagctcg gccgcgtgca
     5821 ggaacatgcg cttgaggccg cgcttcacca gcgccttgtt caagccgaag tcgccgtact
     5881 tgtcgtcacc ggcgatcgga aagccgaggt gcgccaggtg cacgcgaatc tggtgcgtgc
     5941 gcccggtccg cagctgcgcc gccagcaggc tgaagccggg aaggtgccgc tgcagcgtga
     6001 actcggtgtg cgacggcatg ccgcccgcct ccacgctcac tcgccgctcg ccttgcgcgg
     6061 tcaggcggcg caccagcggc agcttcacga cctgtcgcgc actgcgccaa cggccggcga
     6121 ccaggacgag atagcgcttc tccaccaggc cgtcgcgcag ctgccggtgc agctcgatca
     6181 gcgcgctgcg cttcttggcg agcaacagga cgcccgaggt gtcgcggtcg agccggtgca
     6241 ccagctcgag gaagcgcaac gccggccgct ccagacgcag ctgctcgatc gcgccgcggc
     6301 tgaccccgct gcccccgtgc accgcgaccc ccgccggctt gtcgatcgcc agcaaccact
     6361 cgtcttccag gagcgggcgg cgacccagca gcggcgcca
//
LOCUS       DASPGE010000254        15405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1238154, whole genome shotgun sequence.
ACCESSION   DASPGE010000254 DASPGE010000000
VERSION     DASPGE010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15405
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238154"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VD791_08185"
     CDS             <1..336
                     /locus_tag="VD791_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07994.1"
                     /translation="GKLRAERCQARLPKRNTQWLDLVQAVAVRDSARMTALASAMLEE
                     ADAEAKTYLVHAAMLGALARDRSDEAVAIWRAHGEKLHNGTVPLALDTRVLVALAHMN
                     PMQQAGLTR"
     gene            complement(448..1878)
                     /locus_tag="VD791_08190"
     CDS             complement(448..1878)
                     /locus_tag="VD791_08190"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXV07995.1"
                     /translation="MTAPDFFVDVQGGARVCLPPTLSNITTYVLLEQETWFEDELDFV
                     RVCLGPGDCAVDVGANFGVFTTAMAAAVGGGGSVLAFEPAASTAAFLRETLSANGLTQ
                     VQAVQAALSDRPGSALLEIGDSPELNALAEAPQAGREAERVELLRLDDVMRRHGGMQL
                     SLIKIDAEGHELQVSKGAQRTLLAHEPLVLFEIKHGPSVEYRLLDFLQTLGFEAYRLL
                     PGPGVLVPHRRDMPLDDFQLNLFGCTPGRARALNAAGLLALPGAAPAAETADSREARD
                     YLQTLPGPAPAAGSADAHAQALLHYALSQTPAATPEEKAAQLGQALRLARRACSEEAS
                     LARLLTLTALAAGAGERRAAVDTLATLKGMVDVGHAQLSEPCLPPHPRYAALPWRESP
                     VEWVECAVVESFERLCAFSSVFQGPAALPSLEYLRSKRFYCAQMERRRQLLRMRSGRQ
                     PGPQPHPLLIDTAPDNLNPWFWSAGQ"
     gene            complement(1875..3938)
                     /locus_tag="VD791_08195"
     CDS             complement(1875..3938)
                     /locus_tag="VD791_08195"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV07996.1"
                     /translation="MRLLSRLFAAKAAPQPAAPSAPGPDLAALLGAGDAAYQAGERAR
                     ARQQFARALEIDARNAHAQYMLGALALQDGDTQACMTAVRRAIELAPERAEYHFSLGN
                     VHAAAGRTAEAIDCYRNAVRLSPGAQDWRDELAITLAEAGRLAEATQAQYPDAGGAGG
                     LYDLASMLMERGRLAAAAAAFRESIRLEPGAAASHLMLAVALRDQGMPVEAEAPAREA
                     VALAPDMPEGWFMLGHVLSRQARHEDAVEHYRKAIELAPDYDAAWRCLVFAMNYSERW
                     TPREVFDAHLESARRFPRTAHVSVGADHLRPGHRLRIGYLSGDLRRHPVAYFLEPVLR
                     HHDHGRFEVFCYHTGADEDDLSARLRAHADHWRAVGRLSPDDLDRTLRADRLDLLVEL
                     SGHSDGHRLAVVARRVAPVQVSYLGYPNTTGLTAIDYRITDAQADPPGEADALHTERL
                     ARLPETFLCYAPPFAGEVAARPPVCSRGRVTFGSFNNFAKLSASNIALWARVLDAVPG
                     ATLFIKTGGLQDPGLRRLVLERFRAAGIDSSRLELAAPTDSIEAHMRSYDRVDIALDS
                     FPYHGTTTTMDALWMGVPVVTLRGDRHAARVSASILHTLGLDQMVAASAQDYVQIARR
                     LALDVAGLETLRRTLRQRLQASPLMDGVRFTRHLEEAYLRMWQAALATPAAESAGERG
                     RPGRA"
     gene            4061..4390
                     /locus_tag="VD791_08200"
     CDS             4061..4390
                     /locus_tag="VD791_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV07997.1"
                     /translation="MKGVAVIVRDWLRRKEIEGFAVLLAQDLKRRFPPDSEKRTDRGV
                     RRQAESIAASLCGKGAEFNRQHGLGIYGKAKLGNTLRWQLRAFGYSEGFVESLVHELV
                     LHLGRVR"
     gene            4445..5584
                     /locus_tag="VD791_08205"
     CDS             4445..5584
                     /locus_tag="VD791_08205"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HXV07998.1"
                     /translation="MEKIPFINGLRGLAIAGTVFHHSFFGMLQYGLAASSLPLLAQVF
                     LSSGWLGVNLFFFLSGFVLYLPYAAGERSMLSRADAGRFYRRRFERLMPLYWFIALVS
                     LVFLTRAELDQPVLYLAMLLYATVTFPFFPQTFMPPGNWVLWSIGVEIWFSLLFPLLV
                     LLLHRFGWRAMLAWTLAVSLGFRLLGQIHFYQPPNVLNFASDSVLGRLDEFVWGMVAA
                     RLFARGEHRKLPAWSFPVGVALVLAAMWLWGRWYRQELPAWSAAFYSWPLDVGIFLAV
                     CHLGRGAGLARSLLENRPLQLLGMMCFSIYVWHGIVILRYAQSFNHGPQYLGYLLVVL
                     ALAFVTYRYVEFRKVSSVRDLLPRAAPRGPAPDMVRPRAGHPPHA"
     gene            5577..6671
                     /locus_tag="VD791_08210"
     CDS             5577..6671
                     /locus_tag="VD791_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXV07999.1"
                     /translation="MPELTVVVPAYRERDNLLTLIAALDKAMQGQDWEAIIVVDDASD
                     GSDTLVRERAQRDRRVRCIQRIGRRGLASACIEGMLASSAPYLAVIDADLQHDETLIP
                     ELLQALKGGDADIVVASRYAPGASTGELAAARVLMSRLAGAVSRVLSPSLTDPMSGFF
                     ALRRSYLDRVVRKLYGRGFKILLDLIAAARGRAQVVELPYRMRQRVHGESKMSTRVIA
                     EFFMLLLYHLFGRLLPARFFLFGAVGLSGVGVHFVALSLLFHASAGRFLLSQVLATWF
                     TMTSNFFLNNAFTYADQRLRGRGLWRGLATFYAACSLGALINVAVAAYLFDAGTDYRV
                     AGVVGAGIAALWNFLTTASVTWGGSSRAER"
     gene            6671..8686
                     /locus_tag="VD791_08215"
     CDS             6671..8686
                     /locus_tag="VD791_08215"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HXV08000.1"
                     /translation="MRQRAPGTEWLHAAGVALVLLALYAASAPRTVALEDDGLFILSS
                     YYLGIEHPPGYPLHTLLGKLFTWLPIGSVAYRVHLLSAVFGALTASAVWLCTWQLVGR
                     RWPAWLAALGLGLSRTFWSQAIIAEVYTLNTFFVFVLLFLGLRATSAAPGARKVLLFM
                     APLAGLSLTNHWPLMLLVAPAFALLLWPVRKQILERLPLLCGLFFAGLLPYAWMVMRS
                     WSGPPISFYGPIESLRELWVMLSRSGYGTEDVSPAATWVDRLKYFRFLGDELLVQFAV
                     AGTLVAAVGFQAQWREWGPRVSASLMLAFLAPTVGLLLLLGFDYDHLRRHVYQVYPLP
                     AYGVMALWMGLGFARLARSTTAGVARAGAAAALLLGLIGALAWDSNMRAAYAWSARYA
                     QALLASLPPDAVLFLHGDAEVGAIGYFHLIEGMRPDVTLHNTKGLVFGNRLFHPLRTD
                     GAGVRSAIRAFLDRERREVAFTGGFPEDQARDDRWLYVVTDRSAGAPGAGFRLDEPMR
                     RFLEESVLPHAEKDPWTLFMQGELRRRIGAILCMTLRQDQAADPTTLRYLALLSTDFD
                     GALGMVEGLLANDAGHSIHQVARLLQTARERMPDDALKSQRARYFELHAYLRLDLGDV
                     QGAIADLETAVALQPFASHRAASALAGLYRQTNDMQALARLQERLVR"
     gene            8702..9601
                     /locus_tag="VD791_08220"
     CDS             8702..9601
                     /locus_tag="VD791_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXV08001.1"
                     /translation="MSLAEFVYTVLLKPPLLRRAANATLRALIPEAVRVGEALIWLNP
                     KDPVVSGALALRVFERGEIDFFRAHLNGDMTFVDVGANVGLYTGLALAKPDFRGRIVA
                     IEPHAESLLFLHKTIRSNGGADWQRRAVLCAVAASDRPGSVTLYKNSQNRGDNRLYAD
                     APLDEQEAVSTDTLDNICSRHGIGSVQFLKLDVQGAEAKVLSGARRVLSGSADCILMS
                     EFWPYGLQRMGADGLLYLELVGDLGFTLFYSPRRANRLVPVTDRRGLTSGLRGRSYTN
                     LVGLKGAFLEQARSDRRRSRAIS"
     gene            complement(9591..10442)
                     /gene="soxA"
                     /locus_tag="VD791_08225"
     CDS             complement(9591..10442)
                     /gene="soxA"
                     /locus_tag="VD791_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HXV08002.1"
                     /translation="MPRARRPLARLALTACAVALAGLCAAASSTPEQDRQRLISFFTD
                     RFPGVALDDYVYGAMIASADARAQYEQIMEFPPFLNDIEAGRKIWETPFRNGRRFADC
                     FPDGGRNAAGDYPRYDERLGRVITFEAALNRCRQANDEPPLAYGEREPMGVLTAYART
                     LSDGMRVNVKVNTPAARVKYEAGRDLYFRRIGQLNASCAGCHVHNAGNTMRMEIISPA
                     LGQATHWPIFRGGEELMTFQGRFKRCMEQMRAVPYGYDSEEWNNLEYYLSYLSRGLPM
                     RSSVFRK"
     gene            complement(10447..10770)
                     /gene="soxZ"
                     /locus_tag="VD791_08230"
     CDS             complement(10447..10770)
                     /gene="soxZ"
                     /locus_tag="VD791_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HXV08003.1"
                     /translation="MAEPIRLRVKLEGDVADVRVLIGHPMETGLRKDPRTGDLVPLHF
                     IKSVTATLNGKPVLTAQWSQAVSRNPYMQFRIKGAKAEDEVGIEWVDNRGESNRARAK
                     VPAAG"
     gene            complement(10775..11242)
                     /gene="soxY"
                     /locus_tag="VD791_08235"
     CDS             complement(10775..11242)
                     /gene="soxY"
                     /locus_tag="VD791_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HXV08004.1"
                     /translation="MNTDPDRRPSRRSFLALSAMLLAAVLRPLHAWAGDWNKAAFDAR
                     NVQDALKSAGIVNPAASADIVIKAPEIAENGAQVPVDILSKVPGTDTVYIFVDKNPQP
                     FVGSFDFLNGAEPFISTRIKMGETSNLRVVVRAGGRHHMATREIKVTIGGCGA"
     gene            complement(11263..11625)
                     /gene="soxX"
                     /locus_tag="VD791_08240"
     CDS             complement(11263..11625)
                     /gene="soxX"
                     /locus_tag="VD791_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HXV08005.1"
                     /translation="MGAERAVVSGVAIALLAAAAPSAGTETAAVAAGRALAHDVYKGN
                     CLGCHQVPGDRAAISLANIGPPIVAMRERFPNRAALREQLWDSTVRNPYSVMPPFGKH
                     RVLTEEEIDLVLEYIYRY"
     gene            complement(11718..12212)
                     /gene="moaC"
                     /locus_tag="VD791_08245"
     CDS             complement(11718..12212)
                     /gene="moaC"
                     /locus_tag="VD791_08245"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HXV08006.1"
                     /translation="MGELSHFDGRGQAHMVDVGDKAETERVAVATGGVVMRPETLQRI
                     RAGTAAKGDVLGVARIAAIQGAKRTAELIPLCHPIALTHVSVDFSFDEARSAVRCTAT
                     AHSRGRTGVEMEALTAVTVGLLTIYDMCKAVDRGMRFEGVRLLEKRGGKSGHWVAPAE
                     VPGT"
     gene            12283..13707
                     /locus_tag="VD791_08250"
     CDS             12283..13707
                     /locus_tag="VD791_08250"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXV08007.1"
                     /translation="MRFLVFLLAALLIAAARAETLPDLGDSSQTVLSTQQERQLGEAI
                     MRQIRASKHYLDDPEIGDYINGLGYRLVAHSPDADQPFEFFLVDDRSINAFALPGGFI
                     GVHSGLIVSSESESELAAVLAHEVAHVTQRHIARLFQQQKQAGIASLAAIALAILAAR
                     SSPDLATAALAGAQYATVQTQLNFSRDNEREADRVGVQVLERSGLDPRAMPSFLERLQ
                     RAYRLYETNAPSYVRTHPLTYERIADVQSRVESMPYRQVPDTLEFQLVRARLQVRTSG
                     PREAVERFEDILREKKTVSETAARYGLILALLEAKELQRARRELPALEREAPADPMVL
                     ALAAQVRKASSDTKGALALYADAVRRHPRRRALIYEYAQSLLDARRPQEALDLITDAV
                     RALPPDARLYRLQAQSHAALGQQLQQHRAQAEAYLLMGSLPAAIEQLQIGLKSGDGDY
                     YQLSSAEARLRELRALDQEARRRP"
     gene            13907..15169
                     /locus_tag="VD791_08255"
     CDS             13907..15169
                     /locus_tag="VD791_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV08008.1"
                     /translation="MKELGRGATSAVYLAHDPFANRKVALKVVQRDVLGDREHGKRFR
                     KLFLTEASLVGKLSHPHIVAIYDAVADEEGSYIVMEYVEGGTLEQHTKIDNLLPINKV
                     IEIVFKCCKALDYAHRHGVIHRDIKPANILLSKDTDIKISDFGAALTVASETTQVTGI
                     GSPAYMSPEQVQEHQLTHQTDIFSLGVVMYQMLTGSLPFKATNNYSMVFQIINVDPPP
                     PSIFRPEVPASVDHIVKKALQKRLENRYQTWDELAQDLVRVFNNLQTPEQVVPDTEKF
                     NTLRRLEFFRGFTDVDLWQVLHIANWNRVAAGTTLIREGETGTAFYVLAAGEVRVTKS
                     EKLLDVLKAGECFGEMAYLGRRQFKRSASVHASSEITVIEIKADALNAATESCRHQFT
                     AAFLELLVARLENADVRLSELLERHLVT"
BASE COUNT         2287 a         5364 c         5251 g         2503 t
ORIGIN      
        1 ggcaagctgc gcgccgaacg ctgccaggcg cggctgccca agcgaaacac gcagtggctc
       61 gacctggtgc aggcggttgc ggtgcgcgac tccgcccgca tgaccgcact cgcgtccgcg
      121 atgctcgagg aagccgacgc cgaagccaag acctatctgg tgcacgcggc catgctgggc
      181 gcgctggccc gcgatcgatc cgacgaggcg gtcgcgatct ggcgcgcgca cggggagaag
      241 ctgcataacg ggactgtccc gctcgcgctc gacacgcggg tgctggtcgc cctggcgcac
      301 atgaacccga tgcagcaggc cggcctcaca cgctagtcat cgggtacagc accgacggca
      361 ccgcgcacgt gctgcacgcg gccgggttcc gggggagctc agcgcggaca ccgtgtacgc
      421 ctggcggatc gcggcttccc ggtgccctca ctggcccgcc gaccagaacc acgggttgag
      481 gttgtcgggc gcggtatcga tcagcaacgg atgcggctgc ggtcccggct gccggccgct
      541 gcgcatgcgc agcagctggc ggcgtcgctc catttgcgca cagtagaagc gtttggaccg
      601 caggtactcg agcgacggca gagcggcggg tccctggaac accgagctga aagcgcacag
      661 ccgctcgaag ctctccacca cggcgcactc cacccactcg accgggcttt cgcgccacgg
      721 tagcgccgca taccgcgggt gaggcggcag gcaaggttcg ctgagctgag cgtggccgac
      781 gtccaccatg cccttgagcg tggccagggt gtccacggcg gcacgccgtt cacccgcgcc
      841 ggccgcgagc gctgtcaggg tcagcagccg cgcgagcgac gcctcctcgc tgcacgcgcg
      901 tcgcgcgagg cgaagcgctt gccccagttg agcggccttc tcctccggcg tcgcagccgg
      961 ggtctgcgac agcgcgtagt gcagcagcgc ttgcgcgtgc gcatccgccg agcccgcggc
     1021 aggcgccgga ccaggcagcg tttgcagata atcgcgcgcc tcccggctgt cagccgtctc
     1081 cgccgcgggc gccgcaccgg gcagcgcgag cagccccgcg gcattgagcg cgcgcgcgcg
     1141 tcccggagtg cagccgaaca ggttgagctg gaagtcgtcc agcggcatgt cgcgtcgatg
     1201 cggaaccagc acgccggggc ccggcagcag ccgataggct tcgaacccca gggtctgcag
     1261 gaaatcgagc aggcggtact cgaccgacgg tccgtgcttg atctcgaaca gcaccagcgg
     1321 ctcgtgcgcc aggagcgtgc gctgcgcacc cttgctcacc tgcagctcgt ggccctcggc
     1381 gtcgatcttg atcagcgaca gctgcatgcc gccatgcctg cgcatcacgt cgtccagccg
     1441 caacagctcc acgcgctcgg cttcgcgccc cgcctgcggc gcctcggcca gggcgtttag
     1501 ctcggggctg tccccgatct cgagcagcgc cgacccgggg cgatccgaca acgcggcctg
     1561 caccgcctgc acctgggtca ggccgttggc ggatagcgtc tcgcgcagga acgcggcggt
     1621 cgaggccgcc ggctcgaagg ccagcaccga tcccccgccg ccgaccgcgg ccgccatggc
     1681 ggtcgtgaat acgccgaagt tcgcgcccac gtccacggcg cagtctcccg gcccgaggca
     1741 gacgcgcacg aaatcgagct cgtcttcgaa ccaggtctcc tgctccagca gcacgtaagt
     1801 cgtgatgttg gacagcgtcg gaggcaggca cacccgcgcg ccgccctgca catcgacgaa
     1861 aaagtcgggg gcggtcacgc gcgtcccggg cggccgcgct cgcccgctga ttccgcggcc
     1921 ggcgtggcga gggccgcctg ccacatccgc aggtaggcct cctcgaggtg gcgcgtgaag
     1981 cgcacgccgt ccatcagcgg cgatgcctgc aggcgctggc gcagggtacg acgcagcgtc
     2041 tccagcccgg cgacgtccag cgccagcctc cgggcgatct gcacgtagtc ctgcgcagag
     2101 gccgccacca tctgatccag gcccagcgtg tgcaggatcg atgcgctgac gcgtgcggcg
     2161 tgccggtccc cgcgcagagt gaccaccggc acccccatcc acagcgcgtc catggtggtg
     2221 gtagtcccgt gataggggaa actgtccagc gcgatgtcca cacggtcgta gctgcgcatg
     2281 tgcgcttcga tgctgtcggt cggcgcggcg agctcgagac gcgaactgtc gatgcccgcc
     2341 gcgcggaagc gctcgagcac cagccgacgc agccccggat cctgcaggcc gccggtcttg
     2401 atgaacagcg tcgcgcccgg cacggcatcg agcacgcgcg cccacaacgc gatgttggaa
     2461 gcgctgagct tggcgaagtt gttgaaggag ccgaacgtga cgcgcccgcg gctgcacact
     2521 ggcggccgcg cggcgacctc gccggcgaac ggcggcgcat agcacaggaa ggtctcgggc
     2581 aggcgcgcca gccgctccgt gtgcagcgcg tcggcttctc cgggcgggtc cgcctgcgca
     2641 tcggtgatgc gatagtcgat tgcggtgagg ccggtggtgt tggggtagcc cagataggac
     2701 acctgcaccg gggctacgcg gcgcgccacc accgcaaggc ggtggccgtc gctgtggccg
     2761 gagagctcca ccagcaggtc gaggcggtca gcgcgcagcg tccggtccag gtcatcgggc
     2821 gacaggcgcc cgacggcgcg ccagtggtca gcgtgcgcgc gcaggcgcgc gctgaggtcg
     2881 tcctcgtccg cgccggtgtg gtagcagaac acctcgaagc ggccgtggtc gtgatgccgc
     2941 agcaccggct ccaggaagta cgccaccggg tgacggcgca ggtcgccgga cagatacccg
     3001 atgcgcagcc gatggccggg ccgaaggtga tccgcaccga cggacacatg tgctgttcga
     3061 gggaagcgcc tggccgactc gaggtgcgcg tcgaacacct cgcgcggcgt ccagcgctcc
     3121 gagtagttca tggcgaacac caggcagcgc caggcggcat cgtagtcggg cgccagctcg
     3181 atcgccttgc ggtaatgctc gacggcgtcc tcgtgccgcg cctgcctgga caggacatgg
     3241 ccgagcatga accatccctc gggcatgtcg ggggcgagtg cgaccgcctc gcgcgccggc
     3301 gcttcggcct cgaccggcat gccctggtcg cgcagcgcga cggcgagcat caggtggctt
     3361 gccgccgcgc ctggctcgag ccggatcgat tcccggaacg cggcggccgc cgcggccagg
     3421 cgtcctcgct ccatcaacat cgaggcaagg tcgtagagcc cgcctgcacc gccggcgtcg
     3481 gggtactgcg cctgcgtcgc ctccgccagg cggccggcct cggccagcgt gatggccagc
     3541 tcgtcgcgcc agtcctgcgc cccagggctg aggcgcaccg cattgcgata gcaatcgatg
     3601 gcctccgcgg tccgccccgc ggcggcgtgc acgttgccca gcgaaaagtg gtactcggcc
     3661 cgttccggag ccagctcgat cgcgcggcgc accgccgtca tgcaggcttg cgtatcgcca
     3721 tcctgcagcg ccagcgcgcc cagcatgtac tgcgcgtgcg cgttgcgcgc atcgatctcc
     3781 agcgcacgag cgaactgctg cctggcgcgg gcgcgctcgc cggcttgata ggctgcgtcg
     3841 ccggcgccga gcagcgccgc caggtcgggg ccgggggccg agggggccgc aggctgcggc
     3901 gcagccttgg cggcgaacag tcgtgacagc agcctcatgg taggagcagc gccggggatg
     3961 cggctgccgg gagcaggaac gatgccaaag gctgcacgtg agagcatccg atctggtctc
     4021 gcggcatgct ccgtgcttgc cgtgcgtgcg ccgcaccggg atgaaagggg tcgccgtgat
     4081 cgttcgagac tggcttcgcc gcaaggagat cgagggcttc gctgtcctgc tcgcgcagga
     4141 cctgaagcga cgcttcccgc ccgacagcga gaagcgcacg gaccgcggcg tgcgtcgcca
     4201 ggccgagtcg atcgcggcga gcctgtgcgg caagggcgcg gaattcaacc gccagcacgg
     4261 gctcggcatc tacggcaagg ccaagctggg gaacaccctg cgctggcagc tgcgcgcgtt
     4321 cggttacagc gagggcttcg tcgaatcgct ggttcacgag ctggtgctgc acctgggccg
     4381 ggtgcgctga aggctggttc gcgcgagatc ttccgctgct gacccgagtg taaccgagga
     4441 cggcttggag aagatcccct tcatcaacgg cttgcgcgga ctcgcgatcg cggggacggt
     4501 gttccaccat tccttcttcg gcatgttgca atacggcctc gcggcttcgt cgctcccgct
     4561 gctggctcag gtgttcctgt ccagcggctg gctgggcgtg aacctgttct tcttcctctc
     4621 ggggttcgtc ctctacctgc cctacgccgc gggcgagcgc tccatgttgt cgcgagccga
     4681 cgccgggcgc ttctaccgtc ggcgcttcga gcggctgatg cccttgtact ggttcatcgc
     4741 cctggtgtcg ctcgttttcc tgacccgcgc cgagctggac cagcccgtct tgtatctggc
     4801 gatgctgctg tatgccacgg tcaccttccc attcttccct cagacgttca tgccgccggg
     4861 caactgggtg ctgtggtcga tcggcgtcga aatctggttc tcgctgctgt tcccgctgct
     4921 cgtcctgttg cttcatcgct tcgggtggcg ggcgatgctg gcctggacgc tggccgtatc
     4981 gctcggcttc agactgctcg ggcagattca tttctaccag cctccgaacg tcctgaactt
     5041 cgcgagcgat tcggtgctcg gccgtctgga cgagttcgtg tggggcatgg tcgccgcgcg
     5101 cctgttcgcc cggggcgaac accggaagct gcccgcctgg tccttccccg tgggggttgc
     5161 gctggtcctg gccgcgatgt ggctgtgggg tcggtggtac cgccaggagc tgcccgcgtg
     5221 gagcgccgcg ttctattcct ggcccctcga cgtggggata ttcctggcgg tttgccacct
     5281 cgggcgcggg gcaggtctgg cgagaagcct cctcgagaac cggccgctgc agttgctcgg
     5341 catgatgtgc ttcagcatct acgtatggca tgggatcgtg atactgcgct atgcccagtc
     5401 gttcaaccat ggcccgcagt atctcggtta cctcttggtg gtgctggcgc tcgctttcgt
     5461 gacctatcgc tacgtcgaat tccggaaggt gagcagcgtg cgcgacctcc tgcctcgcgc
     5521 ggcaccccgc gggccggccc cagacatggt ccgcccccgc gccggccacc cgccgcatgc
     5581 ctgagctgac ggtcgtcgtc cccgcctacc gggagcgcga caatctgctg actctgatcg
     5641 ccgcgctcga caaggcgatg caggggcagg actgggaggc gatcatcgtc gtggacgatg
     5701 cgtcggacgg ctcggacacg ctggtccgcg aacgtgcgca gcgcgaccgg cgcgtgcgct
     5761 gcatccagcg catcgggcgg cgcggcctgg cctcggcctg catcgagggc atgctcgcca
     5821 gttccgctcc gtatctggcg gtgatcgacg cggacctgca gcacgacgag acgctcatcc
     5881 ccgagctgct gcaagcgcta aaggggggcg acgccgatat cgtggtggcc agccgctacg
     5941 cgcccggcgc cagcaccggc gagctggcgg ccgcacgcgt cctgatgagc cgccttgcgg
     6001 gcgcagtcag ccgcgtgctc agcccgagcc tgaccgaccc gatgagcggc ttcttcgcgc
     6061 tgcgccgatc ctacctcgac cgcgtcgtgc gcaagctcta cggccgggga ttcaagatcc
     6121 tgctcgacct gatcgccgcc gcgcgcggcc gcgcccaggt cgtcgagctg ccgtaccgca
     6181 tgcgccagcg cgtgcatggc gagagcaaga tgagcacacg ggtgatcgcc gagttcttca
     6241 tgctcttgtt gtatcacctg ttcgggcgcc tgctgcccgc gcgcttcttc ctgttcggcg
     6301 cagtcgggct gagcggcgtg ggcgtgcatt tcgtggcgct ctcgctgctg ttccatgcct
     6361 ccgcgggccg gttcctgctg agccaggtgc tcgcgacctg gttcacgatg accagcaact
     6421 tcttcctcaa caacgcgttc acctatgccg accagcggct gcgcgggcgc ggcctgtggc
     6481 gcgggctagc caccttctac gccgcctgtt ccctgggtgc gctgatcaac gtcgcggtcg
     6541 ccgcgtacct gttcgatgcc gggaccgact atcgtgtcgc cggagtcgtc ggggcgggga
     6601 tcgccgcgct gtggaacttc ctcacgaccg cgtcggtgac ctggggcggg tcgagccgcg
     6661 cggagcgctg atgcggcagc gagcgccggg taccgagtgg ctgcacgcgg cgggggtggc
     6721 gctcgtcctg ctcgcgctct acgcggccag cgcgccgcgc acggtggcgc tcgaagacga
     6781 cggtctgttc atcctgtcca gctactacct cggtatcgag catccgcccg gatacccgct
     6841 gcacaccctt ctcggcaagc tcttcacctg gctgccgatc ggatcggtcg cctaccgggt
     6901 gcacctgctg agcgcggtgt tcggcgcgct caccgcgagc gcggtatggc tctgcacctg
     6961 gcagctggtc ggtcggcggt ggccggcctg gctggcggcg ctcggactgg ggctgtccag
     7021 gacgttctgg tcccaggcga tcatcgccga ggtgtacacc ctgaacacgt tcttcgtgtt
     7081 cgtcctgctg tttctcgggc tgcgcgcgac atccgcggcg ccgggcgcgc gcaaggtcct
     7141 gctgttcatg gcgccgctgg cggggctcag cctgaccaac cactggccgc tcatgctgct
     7201 ggttgcgccc gcgttcgcgc tgctgctgtg gccggtgcgc aagcagatcc tggaacggct
     7261 gccgctgctg tgcggcctgt ttttcgccgg gctgctgccc tatgcctgga tggtgatgcg
     7321 ctcctggagc gggcccccga tcagctttta cggtccgatc gagtccctgc gcgagctgtg
     7381 ggtgatgttg agccgctccg gctacggaac cgaggacgtc tcgcccgccg caacctgggt
     7441 ggaccggttg aagtacttcc ggtttctcgg cgacgaactg ctggtccagt tcgcggtggc
     7501 cggcacgctg gtcgccgcgg tcggcttcca ggcgcagtgg cgggagtggg gcccgagggt
     7561 ttccgcgtcc ctgatgctgg cgttcctcgc gcctacggtc ggcctgctgc tgctgctggg
     7621 cttcgactac gatcacctgc gccgccatgt gtaccaggtg tatccgctgc cggcgtacgg
     7681 ggtgatggcg ctgtggatgg gactggggtt cgcccgtctg gcgcgcagca ccacggccgg
     7741 cgtcgcacgc gccggcgcag cggctgcgct gttgctcggg ctgatcgggg cgctcgcctg
     7801 ggatagcaat atgcgcgccg cctacgcctg gtcggcgcgc tatgcgcagg cgctgctcgc
     7861 ctcgctgccg cccgatgccg tgctgttcct gcacggagac gcggaggtcg gcgcgatcgg
     7921 ctatttccat ttgatcgaag gcatgcggcc cgacgtcacc ctgcacaaca ccaagggcct
     7981 ggtcttcggg aaccgcttgt ttcacccctt gcgtaccgac ggcgcgggtg tgcgttcggc
     8041 gatacgtgca ttcctggacc gcgagcgccg cgaggttgcg ttcaccggcg gtttccccga
     8101 ggaccaggcg cgcgacgatc gctggctcta cgtcgtcacg gaccggtccg cgggcgcgcc
     8161 tggcgccggg ttccggctgg acgagccgat gcgcaggttc ctcgaggagt cggttctccc
     8221 gcatgccgag aaggatccct ggaccctctt catgcagggc gagttgcgac gcaggatcgg
     8281 cgccatcctg tgcatgacgc tgcgccagga ccaggccgcc gacccgacca ccctgcgcta
     8341 tctcgcgctg ctgtcgacgg acttcgacgg cgcgctcggc atggtcgagg gcttgctggc
     8401 taacgacgcc ggccattcca tccaccaggt cgcgcgcctg ctgcagaccg cgcgcgaacg
     8461 gatgccggac gatgcgctga agagccaaag ggcgcgctac ttcgagctgc acgcctatct
     8521 gcgcctcgat ctgggcgacg tgcagggcgc catcgccgat ctcgagaccg ccgtggcgtt
     8581 gcagccgttc gcctcccacc gcgcggcgtc cgcgctcgcg gggctctacc ggcagaccaa
     8641 cgacatgcag gcgctggcgc gactccagga acgtctcgtc cgctgacgta tcgagcgcac
     8701 cgtgtcactc gcggagttcg tgtacaccgt cctgctgaaa ccgccgctgc tgcgtcgcgc
     8761 ggcgaacgcc acgctgcgcg cgctcattcc cgaagccgtt cgcgtcggcg aagcgctgat
     8821 ctggctcaat cccaaggatc cggtcgtctc cggcgcgctg gcgctgcgcg tcttcgagcg
     8881 cggcgagatc gacttcttcc gcgcgcactt gaacggcgac atgaccttcg tggacgtggg
     8941 tgcgaacgtg ggcctgtaca ccgggctggc gctggcgaag ccggatttcc gtggacgcat
     9001 cgtggccatc gagccgcatg ccgagagtct cctgttcctg cacaagacga tccggagcaa
     9061 cggcggcgcg gattggcagc gaagggcggt cctgtgcgca gtcgcggcat cggaccggcc
     9121 cggctccgtg acgctgtaca agaactccca gaacagaggc gacaaccgcc tgtacgccga
     9181 cgcgccgctc gacgaacagg aggcggtgtc gaccgacact ctggacaaca tctgcagccg
     9241 tcacggcatc gggtcggtgc agttcctgaa gctggacgtc cagggagcgg aggcaaaggt
     9301 gctgagcggc gcaaggcgcg tcctgtccgg cagcgccgac tgcatcctga tgagcgagtt
     9361 ctggccgtac ggcctgcaac ggatgggcgc cgatggcctg ctctacctgg agctggtcgg
     9421 cgacctcggc ttcacgttgt tttactcgcc gagaagggcg aaccggctcg ttcccgtcac
     9481 cgaccggcgc ggactgacat ccggattgcg cgggcgcagc tacaccaatc tggtcggctt
     9541 gaaaggggcg ttcctggagc aggccaggtc ggaccgccgc cgaagccgtg ctatttcctg
     9601 aacaccgagg agcgcatcgg cagcccgcgg ctcaggtagg agaggtagta ctcgaggttg
     9661 ttccactcct cgctgtcgta gccgtagggc acggcgcgca tctgctccat gcagcgcttg
     9721 aagcggccct ggaaggtcat cagctcctca ccgccgcgga agatcggcca gtgcgtcgcc
     9781 tggcccaggg ccggcgagat gatctccatg cgcatggtgt tgcccgcgtt gtgcacgtgg
     9841 cagccggcgc aggaggcgtt gagctggccg atgcggcgga agtagaggtc cctgcccgcc
     9901 tcgtacttga cgcgtgccgc gggcgtgttt accttcacgt tcacgcgcat gccgtccgac
     9961 agcgtgcgcg cgtacgcggt gagcacgccc atcggctcgc gctctccgta ggcgagcggc
    10021 ggctcgtcgt tggcctgccg gcaccggttc agcgccgcct cgaaggtgat cacccgaccg
    10081 agccgctcgt cgtagcgcgg gtagtcgccc gcggcgttgc gcccgccgtc ggggaagcag
    10141 tcggcgaagc gcctgccgtt gcgaaacggc gtctcccaga tcttcctgcc cgcctcgatg
    10201 tcgttcagga acggcgggaa ctccatgatc tgctcgtact gggcacgcgc gtcggcgctc
    10261 gcgatcatgg cgccgtagac gtaatcgtcg agcgcgacgc cgggaaagcg atcggtgaag
    10321 aaggagatca ggcgctggcg atcctgttcc ggtgtgctgg acgccgcggc gcacagaccg
    10381 gccagggcaa cggcgcaggc ggtcagcgcg agccgcgcca gcggccgccg cgcgcgcggc
    10441 aaatcgtcag ccggcggccg ggaccttggc gcgcgcacgg ttgctctcgc cgcggttgtc
    10501 cacccattcg atgccgacct cgtcctcggc cttcgcccct ttgatgcgga actgcatgta
    10561 gggattgcgc gagaccgcct ggctccattg cgccgtgagc accggcttgc cgttgagcgt
    10621 ggcggtcacc gacttgatga agtgcaaggg caccaggtcg ccggtcctcg ggtccttgcg
    10681 caagccggtc tccatcgggt gaccgatgag cacgcgcaca tcggcgacgt cgccctcgag
    10741 cttcacgcgc aaacggatcg gttcggccat cggctcacgc cccgcagccg ccgatggtga
    10801 ccttgatctc gcgcgtggcc atgtggtgcc tgccgccggc gcgcaccacc acgcgcaggt
    10861 tcgaggtctc gcccatcttg atgcgcgtcg agatgaaggg ttcggcgccg ttcaggaagt
    10921 cgaagctgcc aacgaacggc tgcggattct tgtccacgaa gatgtacacg gtgtcggttc
    10981 cgggcacctt gctcaggatg tcgaccggca cctgggcgcc gttctcggcg atctccgggg
    11041 ccttgatcac gatgtcagcg ctcgccgcgg ggttgacgat gccggccgac ttgagcgcgt
    11101 cctggacgtt cctggcatcg aaggcggcct tgttccagtc tccggcccag gcgtgcagcg
    11161 gccgcaggac cgcggccagc agcatcgcgg acagggccag gaaggagcgc cgggaggggc
    11221 gccggtcggg atcggtgttc aacgccgtac tctgcgccgc attcaatagc ggtagatgta
    11281 ttccagcacc agatcgattt cctcctcggt gagcacccgg tgcttgccga agggcggcat
    11341 cacgctgtag ggattgcgca ccgtggagtc ccacaactgc tcgcgcagtg ccgcgcggtt
    11401 cgggaagcgc tccctcatgg ccacgatcgg cggaccgatg ttggccagcg agatcgccgc
    11461 gcgatcgccg ggcacctggt ggcagcccag gcagttgccc ttgtacacgt cgtgggcaag
    11521 cgcccggccc gccgccacgg cggcggtttc ggtcccggca gacggcgctg ctgccgccag
    11581 cagcgcaatc gcgacaccag agacgactgc acgctctgca cccatcgcac ccgaacgcgg
    11641 ccagcagggc cgtcagccgg acacctcgaa tctcgcatgc gccggcgtcg catccgggga
    11701 tgttgcgccc gacgcgttca cgtcccgggt acctccgccg gagcgaccca gtggcccgac
    11761 ttcccgccgc gtttctccag cagccgcacg ccttcgaacc gcatcccccg gtccaccgcc
    11821 ttgcacatgt cgtagatggt cagtaagccc actgtcaccg cggtcagcgc ctccatctcc
    11881 acgcccgtgc gtccgcggct gtgcgcggtc gccgtgcacc gcactgcgct acgcgcctca
    11941 tcgaagctga agtccacgga aacgtgcgtg agcgcgatgg gatgacacaa cgggatcaac
    12001 tcggcggtgc gcttggcgcc ctggatggcg gcgatgcgcg ccaccccgag cacgtcgccc
    12061 ttggccgcgg tgccggcccg gatgcgctgc aaggtctcgg ggcgcatcac cacgcctccg
    12121 gtggccaccg ccacgcgctc ggtttcggcc ttgtcgccga cgtctaccat gtgcgcctgg
    12181 ccgcgcccgt cgaaatgcga caactcgccc atagatagtt gattcctcga tgcgacgcgc
    12241 accgtgccgg gcgcccacga ctgcaacggc tatcatagca ccatgcggtt cctggtcttc
    12301 ttgctggcgg cgctgctgat cgccgccgcg cgcgccgaaa cgctgcccga cctgggcgac
    12361 tcctcccaga cggtcctgtc gacccagcag gaacggcagc tcggcgaggc gatcatgcgc
    12421 cagatccggg cgagcaagca ctatctcgac gaccccgaga tcggcgatta catcaacggc
    12481 ctgggctacc ggctggtggc gcacagcccc gatgcggatc agcccttcga gttcttcctg
    12541 gtggacgatc gcagcatcaa cgccttcgcg ctccccgggg gattcatcgg cgtgcacagc
    12601 ggactgatcg tgagttcgga aagcgagtcc gaactcgccg cggtgctggc ccacgaggtg
    12661 gcgcatgtca cccagcggca catcgcgcgt ctgttccagc agcagaagca ggcgggcatc
    12721 gcctcgctcg ccgccatcgc gctcgccatc ctcgccgcgc gctccagccc ggatctggcc
    12781 accgccgctt tggccggggc gcagtacgcc accgtccaga cccagctcaa cttcagccgc
    12841 gacaatgagc gcgaggccga ccgcgtgggc gtgcaggtgc tggagcgctc cgggctggat
    12901 ccgcgtgcca tgccctcttt cctcgaacgg ctgcagcgcg cctatcgctt gtacgaaacc
    12961 aacgcgccgt cctacgtgcg cacccacccg ctcacctacg agcgcatcgc cgacgtgcag
    13021 agccgggtgg agagcatgcc ctaccggcag gtgcccgaca ccctggaatt ccagctggtg
    13081 cgcgcgcggc tccaggtgcg gacctccggc ccgcgtgagg ccgtcgagcg cttcgaggac
    13141 atcctgcgcg agaagaagac cgtcagcgag accgcggcgc gctacggcct catccttgcg
    13201 ctgctcgaag ccaaggagct gcagcgcgca cgccgcgagc ttcccgcgct cgagcgcgaa
    13261 gcgcccgctg acccgatggt gctggccctc gccgcccagg tgcgcaaggc ctcgagcgac
    13321 accaagggcg cgctcgcgtt gtacgccgac gcggtgcggc gccacccgcg caggcgggcg
    13381 ctcatctacg agtacgccca gtcgctgctg gacgcccggc ggccgcagga ggcattggac
    13441 ctgatcacgg acgccgtgcg cgcactgccg ccggatgcgc gcctgtatcg gctgcaggcg
    13501 cagagccatg ccgccctcgg ccagcagctg cagcagcacc gggcccaggc ggaggcctat
    13561 ctgctgatgg gcagcctgcc ggcggccatc gagcagctgc agatcgggtt gaagagcggc
    13621 gacggcgact actaccagct gtccagcgcc gaggcgcggc tgcgcgagct gcgcgcgctc
    13681 gatcaggagg cgcgccgcag gccatgactg cgggtgcctg gtcgcgagta cctggggggc
    13741 ccattggcac gatacgtgtt tggtcagcgg aggcctctcg gcaggcctcc ctccagactc
    13801 caaccgccaa tctaatcaat tgaaaccaaa cgactttcct gagcgggacg gaacccagcc
    13861 ggcccccgcc gcgagcgcga ctgacaagat cgggaaatac caggtcatga aagagctggg
    13921 aaggggggcc accagcgccg tctacctggc gcacgatccc ttcgccaacc ggaaggtggc
    13981 gctcaaggtc gtgcagcgcg acgtcctcgg cgaccgcgag cacggcaagc gcttccgcaa
    14041 gctgttcctg accgaggcct cgctggtggg caagctgtcc catccgcaca tcgtcgccat
    14101 ctacgacgcg gtggccgacg aggagggcag ctacatcgtg atggagtacg tcgagggcgg
    14161 caccctcgag cagcacacca agatcgacaa cctgctgccg atcaacaagg tgatcgagat
    14221 cgtgttcaag tgctgcaagg cgctcgacta cgcccaccgt cacggcgtga ttcaccgcga
    14281 catcaagccg gcgaacatcc tgctgtccaa ggacaccgat atcaagatct cggacttcgg
    14341 cgccgcgctg acggtggcct ccgagaccac gcaggtgacc ggcatcggct ccccggccta
    14401 tatgtccccc gagcaggtcc aggaacatca gctcacgcac cagaccgaca tcttctcgct
    14461 cggcgtggtc atgtatcaga tgctgaccgg cagcctgccg ttcaaggcca ccaacaatta
    14521 cagcatggtg ttccagatca tcaacgtcga tccgccgccg cccagcatct tccggccgga
    14581 ggtcccggcg agcgtcgacc acatcgtcaa gaaagcgctg cagaagcggc tggagaatcg
    14641 ctaccagacc tgggacgagc tggcgcagga cctggtgcgc gtgttcaaca acctgcagac
    14701 gcccgagcag gtcgtgcccg acaccgagaa gttcaacacc ctgcgccggc tggaattctt
    14761 ccgcggcttc accgacgtcg atctgtggca ggtgctgcac atcgccaact ggaaccgggt
    14821 ggccgccggc accacgctga tccgcgaggg cgaaaccggc accgcgttct acgtgctcgc
    14881 tgcgggcgag gtgcgcgtca ccaagagcga aaagctgctc gacgtgctca aggccgggga
    14941 atgcttcggc gagatggcct acctcggccg gcgccagttc aagcgcagcg cgagcgtgca
    15001 tgccagcagc gagatcacgg tcatcgagat caaggccgac gccttgaacg ccgccacgga
    15061 gagctgccgc caccagttca cggccgcctt cctggagctg ctcgtggcgc ggctggagaa
    15121 cgccgacgtg cggctgtccg agctcctcga acgccacctc gtgacctaac cctcgcgccg
    15181 gggaatccgg ctggcccggc tgcgtcgcgc ggcgaacgcg gctagaatcg atacgatgac
    15241 agccgtgagt cacatcgatc tggccgcact gcagcgcctg caacaaactg ctgcggtgac
    15301 gctggtggac gtgcgcaccg atgcggaggt ggcgcgcggc gtgatcgccg gggccgtgca
    15361 catcccgctt cacctgctgc ccgcgcggtt caccgagatc gatcc
//
LOCUS       DASPGE010000255         6206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1707142, whole genome shotgun sequence.
ACCESSION   DASPGE010000255 DASPGE010000000
VERSION     DASPGE010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707142"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VD791_08260"
     CDS             complement(<1..193)
                     /locus_tag="VD791_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXV08009.1"
                     /translation="MSGTLPYSLEGKRVWVAGHRGMAGSAIVRRLAHEGCEVLTATRG
                     ELDLRRQADVEAWIAHTRPQ"
     gene            complement(204..821)
                     /locus_tag="VD791_08265"
     CDS             complement(204..821)
                     /locus_tag="VD791_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXV08010.1"
                     /translation="MVTSIVEPVRARQRQRGLTYLGLLFFVAVIGVGLAAVGSVWHTT
                     VKREREGELLFVGEQYRRAIKSYYDASPGAKRYPRSLEELLLDPRFPNVRRHLRRIYR
                     DPMTGATQWELVSAPDGAILGVHSSSRDKPLKSAGFAPELAAFAQAETYADWVFAFVP
                     AQAEPLPPGAAPALPQPGGSTLGSDGASAGFREEAGGAKVPVPPK"
     gene            complement(815..1153)
                     /locus_tag="VD791_08270"
     CDS             complement(815..1153)
                     /locus_tag="VD791_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein G"
                     /protein_id="HXV08011.1"
                     /translation="MLAILALLVSIAAPRYFAHVDRAKEAALKQDLSVMRDAIDKFYG
                     DKGRYPTDLAEMVTQRYLRRVPVDPLTESADTWIALPPPPEAQAKGEVYDVKSGAEGR
                     AADGSEYREW"
     gene            complement(1245..1715)
                     /locus_tag="VD791_08275"
     CDS             complement(1245..1715)
                     /locus_tag="VD791_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXV08012.1"
                     /translation="MLARGFTLIELVITVAIVGILAAGLLPLTQLAAQRAKEQELRAA
                     LRQIRTALDAYKQAADESRIERKADASGYPPSLEVLAEGVKDAKSPDGRMIYFLRRVP
                     RDPLFPDASAAADETWGLRSYASPPDDPKEGDDVFDVYSRSGGTGLNGVPYREW"
     gene            complement(1780..3939)
                     /locus_tag="VD791_08280"
     CDS             complement(1780..3939)
                     /locus_tag="VD791_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HXV08013.1"
                     /translation="MREGDTERGLAKLEEAMRANPGNARYQQTYERERALAVARYVSE
                     GDLQRSSQQYAGAELAYQRALKLDPDSGRAKAGIAAIDAARKQNEIVTKARADVAAGG
                     LDEAESALRALLEQSPGQRDARDLLNRIGEQRAEARALQPPPALRGPFKQPITLEFRD
                     ATLKSVFEVMSRASGINFVFDRDVRSDTKINIFVRNTSLDDVVKLILVTNQLERKLLN
                     ENTLLIYPNTPTKQKEYKELVVRSFYLANADVKQALNLVKGMVKTPDVFIDEKLNMLV
                     AKDTPDAMRLIANLVRGLDLAEPEVMLEVEVLELASSRLQELGVNYPDRINIGKPPSG
                     AVDAATDVFVFGDDRVKGWVLNPPIVLDLKAEDSDVKVLANPRIRVKNREKAKVHIGD
                     RVPVITTTSTANVGVSSSVSYLDVGLKLDVESNVYLRDEVSIKIGLEVSNIRRVVDVQ
                     GTLAYELGTRNTSTVLQLKDGETQILAGLISDEDRRTANKVPGLGDFPLLGRLFRTQS
                     DDRRKTEIVLLITPRVVRSLNYPQSAAVDLAVGTDAAIGVTPLRISPTQPGDLAIAPG
                     VPAGQAPGALPRPVPAVPGAEPAVPPAETEPDEEPDAAAAAAAQPSSLLMAAPIAARS
                     GSDIAVSLSLAPGGAASRATAELAYDPLQLEPVGGAISSPGRLPIRIDGSTAVRFRLL
                     LTAGRAQVRVENLVGLDESGGPVPVAAPGPVEIAVTQ"
     gene            complement(4023..4580)
                     /locus_tag="VD791_08285"
     CDS             complement(4023..4580)
                     /locus_tag="VD791_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08014.1"
                     /translation="MQPGRRLRTALLLAALAGTLAAVYWASRLPSGPAADDPVVRVNE
                     PRGERPRSAIEDQAAGGALDLNRLQRAKTAGPTDDLFGARDFTPKRKPVQRPIAQPAA
                     ELAAVPAIPAVPPPPPPPFTYLGRLAEGGRTTVFLAQGDRNLVVQVGDVIDNTYQVDE
                     IGSTLLVLTYLPQNLKQTMSIGAPQ"
     gene            complement(4581..5144)
                     /locus_tag="VD791_08290"
     CDS             complement(4581..5144)
                     /locus_tag="VD791_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08015.1"
                     /translation="MKLLPGGVAGLAWTGKRGLIKLRWPGLTGLTLLVFALAFYAFGT
                     APMRARLAALETQRADLAARIGARGTSAQPATQRGQLSNFYAFFPLTRAMPDLLGAIE
                     RAAKSNGLRLDKGEYRLSREHGFPLARYQVTLPLQGSYGEVRGFVNAVLDAVPAAALE
                     ELALKREEIGEPLVEARVRFVLFLGAE"
     gene            complement(5141..5686)
                     /locus_tag="VD791_08295"
     CDS             complement(5141..5686)
                     /locus_tag="VD791_08295"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HXV08016.1"
                     /translation="MARVPAIGLDFVRPGGGTGRLGPLLLITGALSALAVLEHQRVLA
                     RAAQERTAQVEEMHTLSRRAMPSIAGRDSDTPEVREQIQKANAVLAQINVPWDELFAA
                     VEMAQDGSVGLLAIQPDARARSVTIGGEARSLPALLAYMSRLQGSERLRDVVLASHEV
                     KVKEPGQPVEFVLNARWVEAP"
     gene            complement(5692..>6206)
                     /locus_tag="VD791_08300"
     CDS             complement(5692..>6206)
                     /locus_tag="VD791_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08017.1"
                     /translation="ADAENPALPRDPTPEELAARASGIRLSVLNATGDQGIREFRVIA
                     GVPARGIADEFERRTGKTVVNWQPHTLKIGNDMEPGRFTLAFRSPAGWTAVRSRRSDA
                     ALAEALPAALKQESAAGGSIAAGTLYLVGEDIAQLPPFAIPGWQVARLSEEAPPPSVS
                     PTLVRAVGAG"
BASE COUNT          888 a         2225 c         2112 g          981 t
ORIGIN      
        1 cctgcggacg tgtgtgcgcg atccaggcct cgacgtccgc ctggcggcga aggtccagct
       61 cgccccgagt ggcggtgagc acttcgcagc cctcgtgcgc gagacgccgc acgattgccg
      121 aaccggccat gccgcgatga cccgcgaccc agacgcgctt gccctcgagg ctgtacggca
      181 acgtaccgct catggttgcg gccctatttc ggtgggaccg ggaccttagc accccccgcc
      241 tcctcgcgga agcccgcgga cgcgccgtcg ctgccgagcg tggacccccc cggctgcggc
      301 agcgcaggag cggcgcccgg cggcagcggc tcggcctggg ccgggacgaa cgcgaacacc
      361 caatcggcat aggtctccgc ctgagcgaag gccgcgagct ccggggcgaa acccgcggat
      421 ttgagcggct tgtcccggga gctgctgtgc acgcccagga tggcgccgtc gggcgcgctc
      481 accagctccc actgcgtcgc gccggtcatc gggtcgcgat agatgcggcg gagatggcgg
      541 cgcacgttcg gaaacctggg gtccagcagc agctcctcga ggctgcgcgg gtatcgcttc
      601 gcgccgggac tcgcgtcgta gtagctcttg atcgcgcgac gatattgctc gccgacgaac
      661 aacagctcgc cctcgcgctc gcgcttgacc gtcgtgtgcc acaccgatcc gacggcggca
      721 agccccacgc cgatgacggc cacgaagaac agcaggccca gatacgtcag gccgcgctgt
      781 cgctgccgcg cgcgcacggg ttcgacgatc gacgtcacca ctcgcggtac tcgctgccgt
      841 cggcggctct gccttccgcg ccgctcttga cgtcgtagac ctcgcccttg gcctgcgcct
      901 cgggcggagg cggcaacgcg atccaggtgt cggcgctctc ggtgagcgga tctaccggaa
      961 cgcggcgcag gtatcgctgg gtaaccatct ccgcgagatc cgtcgggtag cgccccttgt
     1021 cgccgtagaa cttgtcgatg gcgtcgcgca tgacgctcag gtcctgcttg agcgcggctt
     1081 ccttcgcgcg atccacgtgg gcgaagtaac gcggcgcggc gatcgacacg agcagcgcca
     1141 ggatcgccag cacgacgatc agctcgatca gggtgaagcc cctttcgcgc cgaccctttc
     1201 ccgcagggag agacggagac tccctcgctt cgcacctcac gcgctcacca ctcccggtac
     1261 ggcacgccgt tgagcccggt accgcccgat cgcgagtaga cgtcgaacac gtcgtcgccc
     1321 tctttggggt cgtctggcgg gctcgcatag ctgcgcagtc cccaggtctc gtcggccgca
     1381 gcgctcgcgt cggggaacag cgggtcacgc ggcacgcgcc gcaggaagta gatcatcctg
     1441 ccgtcgggcg atttcgcgtc tttgacgccc tcggccagca cctccaggct cggcggataa
     1501 cccgaggcgt cggccttgcg ctcgatgcgg ctctcgtcgg ccgcctgctt gtaggcatcc
     1561 agcgcggtgc ggatctggcg cagcgcggcg cgcagctctt gctccttcgc gcgctgcgcg
     1621 gcgagctggg tgagcggcag caggcccgcc gccaggatgc cgacgatcgc aacggtgatc
     1681 accagctcga tcagggtgaa gcctcgagcg agcatccgaa ggccccggat tccgctgcgc
     1741 tgtatccggg ctacatgctt acagctcacg ccttgcgcct cattgcgtca cggcgatctc
     1801 gaccggaccc ggcgccgcca ccggcacggg gccgccgctc tcgtcgaggc cgaccaggtt
     1861 ctcgacgcgc acttgcgcgc gtccggcggt gaggagcagg cggaagcgca cggcggtgga
     1921 tccgtcgatg cggatcggca ggcgcccggg cgaggagatc gcaccaccga ccggctcgag
     1981 ctgcagcgga tcgtacgcca gctcggcggt ggcgcgactg gcggcgccgc cgggcgcgag
     2041 cgaaagcgac acggcgatgt cgctgcccga gcgcgccgcg atcggcgcgg ccatcaacag
     2101 gctgctgggc tgtgcggcgg cggcggcggc cgcgtccggc tcttcatcgg gttccgtttc
     2161 ggcgggcggt acggcgggct ccgcccccgg gacagcgggt acgggccgcg gcagtgcgcc
     2221 gggcgcctga ccggccggca cacccggggc gatggcgagg tcgccgggct gggtgggcga
     2281 aatgcgcagc ggcgtgacgc cgatggcggc gtcggtgccc accgcgagat cgaccgcagc
     2341 gctctgcggg tagttcaggc tgcgcacgac gcgcggcgtg atcagcagca cgatctcggt
     2401 cttgcggcgg tcgtcgctct gcgtgcggaa cagccggccg agcagaggaa aatcgcccag
     2461 ccccggcacc ttgttggcgg tgcggcggtc ctcgtcgctg atcagcccgg ccaggatctg
     2521 cgtctcgcca tccttgagct gcagcacggt ggaggtgttg cgcgtgccca gctcgtaggc
     2581 aagcgtgccc tgcacgtcca ccacgcggcg gatattgctc acctccaggc cgatttttat
     2641 gcttacctcg tcgcgcagat agacgttgga ctcgacgtct agcttcagcc ccacgtccag
     2701 gtagctcacc gaggaggaca caccgacgtt ggcggtggag gtggtagtga tcaccggcac
     2761 gcggtcaccg atgtgcactt tcgccttctc gcggttcttg acgcggatgc gcgggttagc
     2821 gagcaccttt acgtcgctgt cctccgcctt caggtcgagc acgatcggcg ggttcagcac
     2881 ccatcccttc acacgatcgt cgccgaacac gaacacgtcg gtggccgcgt ccaccgcccc
     2941 ggaggggggc ttgccgatgt tgatccgatc cggatagttg acccccagct cctgcaaacg
     3001 gctgctggca agctccagca cctccacctc cagcatcacc tcgggctcgg ccaagtccag
     3061 cccgcgcacc aggttggcga tcaggcgcat cgcatcgggc gtgtccttgg ccaccagcat
     3121 gttgagcttc tcgtcgatga acacgtcggg ggtcttcacc atgcccttta ccaggttgag
     3181 cgcctgcttc acgtcggcgt tggcgagata gaagctgcgt accaccagct ccttgtactc
     3241 cttctgcttg gtcggcgtgt tgggatagat gagcagcgtg ttctcgttga gcagcttgcg
     3301 ttccagctgg ttggtgacca ggatcaattt gaccacgtcg tcgagcgagg tgttgcgcac
     3361 gaagatgttg atcttggtgt ccgagcgcac atcgcggtcg aagacgaagt tgatgcccga
     3421 ggcgcgcgac atcacctcga acaccgattt caaggtggcg tcgcggaact cgagcgtgat
     3481 cggctgcttg aacggccccc tgagcgccgg cggcggctgc agtgcgcgcg cctcggcgcg
     3541 ctgctcgccg atgcggttga gcaggtcgcg cgcatcgcgc tggccggggc tttgctccag
     3601 cagcgcacgc agcgcgctct cggcctcgtc cagcccgccc gccgccacgt cggcacgggc
     3661 cttggtcacg atttcgttct gcttgcgcgc agcatcgatc gcggcgatgc ccgccttggc
     3721 gcgcccgctg tccgggtcga gcttgagggc gcgctggtac gccagctccg cgccggcgta
     3781 ctgctgcgag ctgcgctgca gatcgccctc cgacacgtag cgcgccaccg ccagcgcccg
     3841 ctcgcgctcg taagtctgct ggtaccgggc gttgcctggg ttggcgcgca tcgcctcctc
     3901 cagcttggcc agcccgcgtt cggtatcgcc ctccctcatc agccggcggc tttcctcgta
     3961 ggacctcgaa cccgcgcaag cggcgagcag gaagcaggct aacgccagcg cgcagaggcg
     4021 gatcattgcg gcgctccgat ggacatggtc tgcttcaggt tctgcggcag ataagtcagc
     4081 accagcagcg tggagccgat ctcgtccacc tggtaggtat tgtcgatgac atcgccgacc
     4141 tgcaccacca ggttgcgatc gccctgcgcc aggaacaccg tggtccgccc gccttcggcg
     4201 agccggccca gatacgtgaa tggcggcggt ggcggcggcg gaaccgctgg aatcgcaggc
     4261 accgccgcca gctccgcggc cggctgtgcg atcgggcgct ggaccggctt gcgcttgggc
     4321 gtgaagtcgc gcgcgccgaa cagatcgtcc gtcggtcccg cagtcttggc gcgctgaaga
     4381 cgattcaggt cgagcgcacc accggctgcc tgatcctcga tggcgctgcg cggcctctcg
     4441 ccgcgcggtt cgttcacgcg tacaaccgga tcgtccgccg ccgggcccga tggcagtcgg
     4501 ctcgcccaat acacggcggc gagcgttccc gcgagcgctg cgagcagcag cgcggtgcgc
     4561 agtcggcggc cgggctgcac ctactccgcc cccaggaaca gcacgaagcg cacgcgcgcc
     4621 tcgaccagag gctcgccgat ctcttcgcgc ttgagcgcca gctcctcgag cgcagccgcg
     4681 ggcacggcgt cgagcacggc gttgacgaat ccgcgcacct cgccgtagct gccctgcaga
     4741 ggcaacgtga cctgataccg ggccagcggg aagccgtgct cccggctcag ccggtattca
     4801 cccttgtcga gccgtagccc gttgctcttc gcggcgcgct cgatcgcccc cagcagatcg
     4861 ggcatcgcgc gcgtcagggg aaagaaggcg tagaaattgg agagctggcc gcgctgcgtg
     4921 gccggctgcg cgctcgtccc gcgcgcaccg atgcgcgcag ccagatccgc gcgctgggtc
     4981 tccagcgctg ccaggcgcgc acgcatcggc gcggtgccga acgcgtagaa ggccagcgcg
     5041 aataccagca gcgtcaggcc ggtgagcccc ggccagcgca gcttgatcaa cccgcgcttc
     5101 ccggtccacg cgaggccggc gactccgccc ggcagcagct tcacggcgcc tccacccagc
     5161 gggcgttgag cacgaactcc accggttgcc ccggctcctt caccttgacc tcgtggctgg
     5221 ccagcacgac gtcgcgcaac cgctcgctgc cctgcaggcg gctcatgtaa gcgagcagcg
     5281 ccggcagcga gcgtgcctcg ccgccgatcg tcacgctgcg ggcacgcgca tcgggctgga
     5341 tcgcgagcag gccgacgctg ccgtcctgcg ccatctccac ggcggcgaac aactcgtccc
     5401 agggcacgtt gatctgcgcc agcacggcgt tggccttctg gatctgctcg cgtacttcgg
     5461 gcgtgtcgct gtcccgtccg gcgatcgagg gcatcgcccg gcgcgacagg gtgtgcatct
     5521 cctcgacctg cgccgtgcgc tcctgcgccg cgcgcgccag cactcgctgg tgttccagca
     5581 cagccagcgc gctgagggca cccgtgatca gcagcagcgg cccgagccgt ccggtgccgc
     5641 ccccgggacg cacgaaatcc aatccgatcg cgggcacgcg cgccataccg gctaccccgc
     5701 tcccaccgcg cggaccaggg tgggcgatac ggaaggcggc ggcgcctcct cgctcaggcg
     5761 cgcgacctgc cagcccggga tcgcgaaggg cggcagctgc gcgatgtcct cgccgaccag
     5821 gtacagcgtg cccgccgcga tcgagccgcc cgcggccgac tcctgcttca gcgccgcagg
     5881 cagcgcctcg gcgagcgcgg cgtcgctgcg ccggctgcgc accgcggtcc agccggccgg
     5941 agagcggaac gccagggtga agcgtccagg ctccatgtcg ttgccgatct tcagcgtgtg
     6001 cggctgccag ttgaccaccg tcttgcccgt gcgacgttcg aactcgtctg cgatgccgcg
     6061 cgccggcact ccggcaatca cccggaactc gcgaatgccc tggtcccccg tcgcattgag
     6121 aacgctcagg cgaatgccgc tggcgcgcgc cgccagttcc tcgggcgtcg ggtcgcgcgg
     6181 caaggcgggg ttttccgcgt cagcag
//
LOCUS       DASPGE010000256         3633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1590506, whole genome shotgun sequence.
ACCESSION   DASPGE010000256 DASPGE010000000
VERSION     DASPGE010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590506"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1416
                     /locus_tag="VD791_08305"
     CDS             61..1416
                     /locus_tag="VD791_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HXV08018.1"
                     /translation="MSGTLRIGAGSAWWGDRIEPAALNAARGDLDYLCFETMAEATVS
                     AAQVRARRDPTFPGYDTYLDERMRAVLPHCLKRGTRIVTNQGWINPEGAAQRVVHWLR
                     ELGARGVKVAAIRGGLITDRVLALTDTILENGRPTATLADSLVSAEVYLGAEPIVEAL
                     AQGARIVVSGRVADPSIFMAPMMYEFGWDALDHGRLGPGNGIGHLMECGAQVTGGYFP
                     DPGFKDVPEPWNLAFPIAEVEPDGSAVITKVAGSGGAVNLMTVKEQLLYEVHDPANYL
                     TPDVVVDFTTARLEQVGPDRVCVTGISGKPRTPTLKVSIGCAEGFIGEDMFFYAGPGA
                     LRRAQLAKRILEERFKLVNLQAEELRIDFLGVNAIHGAMSPRDRPEPYEIAVRVAART
                     KTREEAIKVGREVDGMAVSGVGMTGKRVPHQDRTREVIGVWSSLVPRERIVPSIAMFE
                     S"
     gene            1433..1777
                     /locus_tag="VD791_08310"
     CDS             1433..1777
                     /locus_tag="VD791_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08019.1"
                     /translation="MKVLLQHAAHCRSGDKGNISNIVVVAYDAALYPHLKEQLTAARF
                     KDFYAGVIAGPVDRYTIDPLWMMNFVCHGALGGGVSRSLCLDNYGKTLSAGILGFELE
                     IPPALRGCLKGM"
     gene            1902..2144
                     /locus_tag="VD791_08315"
     CDS             1902..2144
                     /locus_tag="VD791_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08020.1"
                     /translation="MEPLVTLANLLFVAAYFVRRMLWLRAFALGGASCLALYFYMLPT
                     PLMQVVYWNLFYVTLNLLWIGRLVLARDGVHSGRSA"
     gene            complement(2173..2430)
                     /locus_tag="VD791_08320"
     CDS             complement(2173..2430)
                     /locus_tag="VD791_08320"
                     /inference="COORDINATES: protein motif:HMM:NF016352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF504 domain-containing protein"
                     /protein_id="HXV08021.1"
                     /translation="MIPIQDFLHRVRWDPEFGRGRLEIAYLDKLAGGLMRVPFTEVRF
                     VPGERFGFEIVGLHGESVMIPYHRVREVYRDGQLVWQRPEP"
     gene            2531..>3633
                     /locus_tag="VD791_08325"
     CDS             2531..>3633
                     /locus_tag="VD791_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXV08022.1"
                     /translation="MDLAFLPAHRLAALVRQKKVGCLELLNLYLDRVEKYNAKLNAIV
                     VLDAERARKRARAADRALAKGEVWGPLHGVPITIKESFDVEGLPTTWGRPELRNNIAP
                     RNALAVERLLQAGAVLFGKSNVPRLLADWQTFNPIHGTTNNPWDLARVPGGSSGGAAA
                     ALAAGLTALETGSDIGASIRNPAHYCGVFGHKPTFGVCSSEGQTLPPYLRESDIAVIG
                     PLARSARDLEIAFKAIAGPDAADATGWQLRLPRAPDKTWRQWKIGVLLDAPNAEVDRS
                     VQERIQGVAEFLARRGAKVDDRARPDIDLAEAHRAFLQLLRAATTRALTDEMFARHLE
                     AARQARPDDDSYYAWMVKANTMHHRDWLYLNEKR"
BASE COUNT          592 a         1259 c         1252 g          530 t
ORIGIN      
        1 aagcacgccg atgcgttacg ctatggaagc ggaccacgac gggccgcgag gaggacgacg
       61 atgagcggga ctctccgcat cggcgccggc tcggcgtggt ggggcgatcg catcgagccg
      121 gcggcgctca acgccgcgcg cggcgatctc gactacctgt gcttcgagac catggcggag
      181 gccaccgtgt ccgcggccca ggtgcgtgcg cggcgcgacc cgacctttcc cggttacgac
      241 acctatctcg acgagcgcat gcgcgcggtc ctgccgcatt gcctgaagcg cggcacccgg
      301 atcgtcacca accagggctg gatcaatccc gaaggtgcgg cgcagcgcgt cgtgcactgg
      361 ctgcgcgagc tgggcgcacg cggtgtgaag gtggcggcga tccgcggcgg gctgatcacc
      421 gaccgcgtgc tggcgttgac cgacaccatc ctggagaacg gccgccccac cgcgacgctg
      481 gcggacagcc tggtgtcggc cgaggtctac ctcggcgccg agccgatcgt ggaggcgctc
      541 gcacagggcg cgcgcatcgt ggtgtccggc cgggtcgccg atccgtcgat cttcatggcg
      601 ccgatgatgt acgagttcgg ctgggatgcg ctggatcacg gccgtctcgg cccgggcaac
      661 ggcatcggtc acctgatgga gtgcggcgcg caggtgaccg gaggttattt cccggatccc
      721 ggcttcaagg acgtgcccga gccgtggaac ctcgctttcc ccatcgccga ggtcgagccc
      781 gacggcagcg cggtgatcac caaggtcgcg ggcagcggcg gcgcggtgaa cctgatgacg
      841 gtcaaggagc agctgctgta cgaggtgcac gacccggcca actacctcac gcccgacgtc
      901 gtggtggact tcaccaccgc ccggctcgag caggtgggcc cggatcgggt gtgcgtcacc
      961 ggcatctcgg gcaagccgcg cacgccgacg ttgaaggtgt cgatcggctg cgccgagggc
     1021 ttcatcggcg aggacatgtt cttctacgcc ggtccggggg cgctgcggcg cgcgcagctc
     1081 gccaagcgca tcctggagga gcgcttcaag ctggtgaacc tgcaggcgga ggagctgcgc
     1141 atcgacttcc tgggcgtgaa cgcgatccac ggcgcgatgt cgccgcgcga ccggcccgag
     1201 ccttacgaga tcgcggtacg ggtggcggcg cgcaccaaga cgcgggaaga ggcgatcaag
     1261 gtgggccgcg aggtggacgg catggcggtg tcgggcgtgg gcatgaccgg caagcgcgtg
     1321 ccgcaccagg accgcacgcg cgaggtgatc ggcgtgtggt cgtcgctggt gccgcgcgag
     1381 cggatcgtgc ccagcatcgc catgttcgaa agctgaagcc cgaggacaat cgatgaaggt
     1441 cctgctgcaa catgcggcgc actgccgctc cggcgacaag ggcaacatct ccaatatcgt
     1501 cgtggtcgcc tacgacgcgg cgctctatcc gcacctgaag gagcagctca ccgccgcccg
     1561 tttcaaggac ttctacgccg gcgtgatcgc gggcccggtg gaccgctaca ccatcgaccc
     1621 cctgtggatg atgaactttg tgtgccacgg cgcgctgggc ggcggcgtgt cgcgcagcct
     1681 gtgcctggac aactacggca agacgctgtc ggccggcatc ctgggtttcg agctggaaat
     1741 cccgccggcc ctgcgcggat gcctgaaggg gatgtgaacc acaccacaga cgcagagcga
     1801 ttcgaaacaa acgcgcaaca aactcgtgcc gcccggaaat cctgcagata cccgcggccg
     1861 ctagactgcg ccgcaatgca atctcggatc ggaaccttgc agtggaaccc ctggtcacgc
     1921 tcgccaacct gctgttcgtc gccgcgtact tcgtgcgcag aatgctctgg ctgcgcgcgt
     1981 tcgcgctggg cggggcgagc tgcctggcgc tgtacttcta catgctgccc accccgctga
     2041 tgcaggtcgt gtactggaac ctgttctacg tcacgctgaa cctgctgtgg atcggccgac
     2101 ttgtcctggc gcgggacggc gtgcacagcg gacgctccgc ctaaacgcgg cacgcgcctc
     2161 gaccagcgcc tctcaaggct ccggccgctg ccagaccagt tgcccgtccc ggtacacctc
     2221 gcgcacgcgg tgatagggga tcatcacgga ctcgccgtgc aggccgacga tctcgaaccc
     2281 gaagcgctcg cccggtacga atcgaacttc ggtgaagggc acgcgcatga gcccgccagc
     2341 cagcttgtcc aggtaagcga tctccagccg gccgcggccg aactcggggt cccagcgcac
     2401 acggtggagg aaatcctgga tcgggatcat cggtggcctc cgggggaagc taccctatca
     2461 tcattccacg ccccgcagcg cggccccgac ctgttatcct gcgcggcgca catcccgcgc
     2521 gcacaccgtc atggacctcg ccttccttcc cgctcatcgc ctcgccgccc tggtgcggca
     2581 gaagaaagtc ggctgcctcg agctgctgaa cctgtatctg gaccgcgtgg agaagtacaa
     2641 cgcgaagctg aacgcgatcg tcgtgctgga cgccgaacgc gcacgcaagc gcgcgcgcgc
     2701 cgccgaccgc gcgctcgcca agggcgaagt gtgggggccg ctgcacggcg tgcccatcac
     2761 catcaaggag tctttcgacg tcgaaggcct gcccaccacc tggggccgcc ccgagctgcg
     2821 gaacaacatc gcgccgcgca acgcgctggc ggtggagcgc ctcctgcagg ccggggcggt
     2881 gctgttcggc aagagcaacg tgccgcgcct gctcgccgac tggcagacct tcaatccgat
     2941 ccacggcacc accaacaacc cctgggacct cgcgcgcgtc ccgggcggat cgtccggcgg
     3001 cgccgcggcg gcgctggcgg ccggcctgac cgctctcgaa accggcagcg acatcggcgc
     3061 ctcgatccgc aacccggcgc actactgcgg cgtgttcggg cacaaaccga cgttcggcgt
     3121 gtgctcctcg gaggggcaga cgctgccgcc ctacctgcgc gagtccgaca tcgccgtgat
     3181 cggcccgctg gcgcgcagcg cgcgcgacct cgaaatcgcg ttcaaggcca tcgccgggcc
     3241 cgacgcggcc gacgccacgg gatggcagct gcggctgccg cgcgcgccgg acaagacctg
     3301 gcggcagtgg aagatcggcg ttctgctcga cgcccccaac gcggaggtcg accgcagcgt
     3361 gcaggagcgc atccagggcg tcgccgagtt cctggcgcgg cggggcgcga aggtggacga
     3421 tcgcgcccgg cccgacatcg atctggccga ggcgcatcgc gcgttcctcc agctgctgcg
     3481 cgcggcgacc acgcgcgcgc tgaccgacga gatgttcgcg cgccacttgg aggcggcgcg
     3541 gcaggcgcgg cccgacgacg acagctatta cgcctggatg gtgaaggcca acaccatgca
     3601 ccaccgcgac tggctgtacc tgaacgagaa gcg
//
LOCUS       DASPGE010000257         9653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1472598, whole genome shotgun sequence.
ACCESSION   DASPGE010000257 DASPGE010000000
VERSION     DASPGE010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9653
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472598"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VD791_08330"
     CDS             <1..451
                     /locus_tag="VD791_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HXV08023.1"
                     /translation="RLLNVGVEGDGALEIASRSRGTPRIANRLLRRVRDYAEVKDRGV
                     VTRALADEALRMLDVDAVGFDVMDRKLLLAVLERFAGGPVGLDSLAAAIGEERDTIED
                     VLEPYLIQQGYLQRTPRGRMATLSAYRHFGIAAPARNASGDLWDAAS"
     gene            438..911
                     /gene="ybgC"
                     /locus_tag="VD791_08335"
     CDS             438..911
                     /gene="ybgC"
                     /locus_tag="VD791_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HXV08024.1"
                     /translation="MRRPERRQLAPPPALFSLPVRVYYQDTDAGGVVYHGTYLDYFER
                     ARTEWLRRLGFDIRRLAELEGTLFIVRELRAAYARPALLDDLLSVTVALEHLGRAQFT
                     LGQRVLRGDEILVQASVNLACVTRVGFKPVRVPDGLRETLEAGPGGRAVPNEERA"
     gene            908..1600
                     /gene="tolQ"
                     /locus_tag="VD791_08340"
     CDS             908..1600
                     /gene="tolQ"
                     /locus_tag="VD791_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HXV08025.1"
                     /translation="MSVTTDLSLLALVSQASLLVKLVMLLLLVVSLVSWWFIFRKSFI
                     LRDTRRRSEDFERTFWSGPDLNQLFQSIVSGKRTAASMDRIFEAGFREFVKLRRQTGM
                     SVAVVMDGTRRAMRATYQREMDYLESHLSFLATVGSVSPYVGLLGTVWGIMNAFRGLA
                     NVQQATLGQVAPGIAEALIATAIGLFAAIPAVVAFNHYAREIEQLATRFETFMEEFSN
                     ILQRQGGGAQPA"
     gene            1612..2034
                     /gene="tolR"
                     /locus_tag="VD791_08345"
     CDS             1612..2034
                     /gene="tolR"
                     /locus_tag="VD791_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018025508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HXV08026.1"
                     /translation="MYERRKRTRLMNQINVVPYIDVMLVLLVIFMVTAPLINTSVIDL
                     PSVSRSNQPPAAPLEVMIQRNGSIVLRDRERGGAERRVDRDTLVYEVKRLQQRNREQP
                     VVIAADENVPYKHVVQVMDLLQANGVARVGLLTRPLSG"
     gene            2044..3039
                     /gene="tolA"
                     /locus_tag="VD791_08350"
     CDS             2044..3039
                     /gene="tolA"
                     /locus_tag="VD791_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HXV08027.1"
                     /translation="MLLDGAREPGRSTSLLLAVAVHVLFFAFLYFGVSWRPKPQPPLS
                     AELWSELPPVRPVKARPEPRIEPPPPKPAPKAEPPPPAAQPKPPPAPTKADIELKEKK
                     QQEKKAAEEARRRQALEREEEEKRRREERARAQEEERRAKEEERQRQEAARREEQQRK
                     REEQERLAELRRQEEAQRKEEERRQDEARREQEVRLEREAEARRSAILDEQQKLAAAA
                     RERAEIDARKRREAEEAAVRARELEAWKSRIHDRIKSKLTLPPGVPDSARAEYSMTII
                     PGGEVLTVKLRKSSGYPAYDAAIERAINAAAPLPVPSDPNLFQQLRELNLVFRPN"
     gene            3062..4408
                     /gene="tolB"
                     /locus_tag="VD791_08355"
     CDS             3062..4408
                     /gene="tolB"
                     /locus_tag="VD791_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HXV08028.1"
                     /translation="MTFLRRPVSIGLDTHRDVVCGRRNALKALGAACLGAATPTAAQL
                     TIEIVGGGADQIPITLVPFGNEERFAQKVSEIVSADLARSGRFKLQEPGSVRPLPTDL
                     PEINYRYWKNRGSQTLVVGKVLERNDGRVEVRFRMTDTVKEAQLVGYSYAILQSQLRA
                     TAHKIADLIYEKLTGEPGVFSTRIAYVARNPNRFELHVADADGYNSQFILAHREPVIS
                     PAWSPDGSRIAYVSFEQRKSIVFLHEMSGQRRILANFEGSNSAPAWSPNGERLALVLS
                     KDGVAQIYTLRAAGGGEPDRLTFSNAIDTEPSFSPDGKWLLFTSDRGGSPQIYRMPAG
                     GGNAERITFEGSYNVTPRYSPDGKSFAFIQRSQGRYSLALMDLAARQIQILTDGQLDG
                     SPTFAPNGRLVMYASIVKGRGILAAVSSDGRIKQKITASALDVREPAWGPLAGNRT"
     gene            4572..5255
                     /gene="pal"
                     /locus_tag="VD791_08360"
     CDS             4572..5255
                     /gene="pal"
                     /locus_tag="VD791_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HXV08029.1"
                     /translation="MKKTLIGLCVALLLAGCGGRGGEKPPASVERREVSDQARSDAAV
                     QAAAEEARRKQAEEDARRLAAEEERRRAEEAARKAAAEQPVVQPLPEAPVAARPLGDP
                     ASVLADPASLLSKRSVYYDFDMYNIREEFQPLVEAHAKFLRENRDLRVRVEGNCDERG
                     SREYNLALGQRRADSIKRAMTLLGVPARQIETVSFGAEKPKAIGSNEEAWAENRRSDI
                     IYVGIDSQQ"
     gene            5305..6156
                     /gene="ybgF"
                     /locus_tag="VD791_08365"
     CDS             5305..6156
                     /gene="ybgF"
                     /locus_tag="VD791_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HXV08030.1"
                     /translation="MLAACALIAATPSAVLAQQPGQQNRNVIELMNQIQALGADLNRL
                     RGELEVLNHSLENAQRRQRDMYLDLDTRLRRLEGTGPEAAQKQDKALADLEARLKRLE
                     EAVAAPAAADELEARVKRLEAQIAPAGAAAPATSSGGAAAASAAPATPRPAAPASEAS
                     PSKRAYDSALSAYRAGDYQGAIAAFDAFVKRYPRDPLAPNAQYWIGDAWFNLRDFRAA
                     ANAQQALISNYPDSPKVPDAMLNLSSSQIALGDSGAARKTLEELVNRHPQSDAAERAR
                     QRLPKLR"
     gene            6239..7372
                     /locus_tag="VD791_08370"
     CDS             6239..7372
                     /locus_tag="VD791_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HXV08031.1"
                     /translation="MLDPAHPVLDPEHLGATIAWLGFGLAFVFGAVAHRVNFCTMGAV
                     SDAVNIGDWGRMRMWLLAIAMAMLAANLLHLAGYVDLERSIYPAERFSWLSYLLGGFL
                     FGVGMTLASGCGSRNLVRLGGGNLKSLVVLTFLAMSAYMTMKGLLAVPRAGVLDPVAL
                     HFDGGQGLPNLLAGLAGMDGRAARWIAMGSVVAALLVFVLKDREFRRSRELILGGVVL
                     GLIIAAGWYVTGYLGYVPEDPQTLEETFAGTNTRRPESFSFVGPVGYSLELLLLWTDS
                     SLRVTFGVAVVAGLLLGSLGYALATRNFRWETFASVADLRNHMLGGVLMGFGGVSAMG
                     CTIGQGLSGVSTLALGSFLALAAIVAGSAVTMKFLYWRLAAKG"
     gene            7467..7542
                     /locus_tag="VD791_08375"
     tRNA            7467..7542
                     /locus_tag="VD791_08375"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7500..7502,aa:Lys,seq:ttt)
     gene            7644..7719
                     /locus_tag="VD791_08380"
     tRNA            7644..7719
                     /locus_tag="VD791_08380"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7677..7679,aa:Lys,seq:ctt)
     gene            complement(7794..9224)
                     /locus_tag="VD791_08385"
     CDS             complement(7794..9224)
                     /locus_tag="VD791_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HXV08032.1"
                     /translation="MDDRSPHLYQELAHRLSDAIHAGSVKAGDRLPSVRQLSTQHRVS
                     IATAVQAYRWLESQRLIEARPKSGYFVLSRGRKLAEPAPSSPPAAARLVGVNRTVMEY
                     LACSETPGVAPLGCATPSEELYPGEKLQRLLGAIGRRRPDLASRYVMSTGNWQLKAAI
                     ARRAVEFGCTLHPEDIVVTNGCTEALNVALRAVAKPGDTIALESPTYFTLLQIIESLG
                     MKALEVPTHPREGISLEALDLATQRPGTVKAVMLITNYSNPLGSLMPDEKKERLVRLL
                     QARGIPLIEDDIYGECYFGRSRPLVAKAWDGAGNVLLCSSFTKSLAPGFRIGWIAPGR
                     FRKEVELLKFISSIATPELPQMAIAQFIANGGYDHHLRRLRAAFLRQVLQVTEAVAEH
                     FPAGCRITQPAGGFVLWVELPPQVDGVRLFELARDAHIAIAPGPMFTNSGKYRNFIRL
                     NCGHALTPRLELAIAKLGDLAKRLAG"
     gene            9403..>9653
                     /locus_tag="VD791_08390"
     CDS             9403..>9653
                     /locus_tag="VD791_08390"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HXV08033.1"
                     /translation="MWVEPIISLTAERSVRIRAKVRTRIGCESGLVWLTRAGDPRDLF
                     LARGEFIELAPGPGVIIATALEATVISVTQPGSRSWRERL"
BASE COUNT         1523 a         3324 c         3346 g         1460 t
ORIGIN      
        1 gcggctgctc aacgtcgggg tcgaaggcga cggcgcgctg gagatcgcct cgcgttcgcg
       61 cggcacgccg cgcatcgcca accgcttgct gcgccgcgtg cgcgactatg ccgaggtcaa
      121 ggaccgcggg gtggtgacgc gggcgctggc agacgaggcg ctacgcatgc tggacgtaga
      181 cgcggtcggc ttcgacgtga tggatcgcaa gctgctgctg gcggtgctgg agaggttcgc
      241 cggcgggccg gtgggcctgg acagcctcgc ggcggccatt ggcgaggagc gcgacaccat
      301 cgaagacgtg ctggagccgt atctgatcca gcagggctac ctgcagcgca ccccgcgcgg
      361 gcggatggcc accctgtccg cctaccgcca cttcggcatt gcggcccccg cgcgcaacgc
      421 cagcggcgat ctctgggatg cggcgtcctg agcgtcggca gctcgcgccc ccgcccgcgc
      481 tgttcagcct gccggtgcgg gtgtactacc aggacaccga tgccgggggt gtggtctacc
      541 acggcaccta cctggattac ttcgagcgcg cgcgcaccga gtggctgcgc cggctcggct
      601 tcgatatccg ccgcctggcc gagctggaag gcacgctgtt catcgtgcgc gagctgcgcg
      661 cggcctatgc ccgcccggcg ctgctcgacg acctgctgag cgtcaccgtg gcgctggagc
      721 acctcgggcg cgcgcagttc accctcggcc aacgggtgct gcgcggcgac gagatcctgg
      781 tgcaggcctc ggtcaacctc gcctgcgtca cccgcgttgg cttcaagccg gtgcgtgtcc
      841 ccgacggcct gcgcgagacc ctggaggccg gcccgggagg ccgcgccgtc ccgaacgagg
      901 agcgtgcatg agcgtcacca ccgatctatc gctgctcgcc ctggtgtccc aggccagcct
      961 gctggtcaag ctggtgatgc tcctgctgct cgtcgtgtcg ctggtgtcct ggtggttcat
     1021 cttccgcaag tccttcatcc tgcgtgacac tcgccgccgg tcggaggact tcgaacgcac
     1081 cttctggagc ggtcccgacc tgaaccagct gttccagtcc atcgtcagcg gcaagcgcac
     1141 cgcggcgagc atggaccgca tcttcgaggc cggcttccgc gagttcgtca agctgcgccg
     1201 ccagaccggg atgtcggtgg cggtggtcat ggatggcacg cgacgtgcaa tgcgggcgac
     1261 ttatcagcgc gagatggact acctggaatc gcacctgtcg ttcctggcca ccgtcggctc
     1321 ggtcagtccc tacgtgggcc tgctggggac ggtgtggggc atcatgaacg cctttcgggg
     1381 gctggccaac gtgcagcagg ccaccctcgg ccaggtcgcg ccgggcatcg ccgaggcgct
     1441 gatcgccacc gccatcggcc tgttcgccgc catccccgcg gtggtcgcgt tcaatcatta
     1501 cgcgcgcgag atcgagcagc tggcgacccg cttcgagacc ttcatggagg agttctccaa
     1561 catcctgcag cgccagggcg gcggcgcgca acccgcttga gggaggcacg catgtacgaa
     1621 cggcgcaagc gcacccgact gatgaaccag atcaacgtcg tgccgtacat cgacgtgatg
     1681 ctggtgctgc tggtgatctt catggtcacc gcgccgttga tcaacaccag cgtcatcgac
     1741 ctgcccagcg tcagccgcag caaccagccg cccgcggcgc cgctggaggt gatgatccag
     1801 cgcaacggca gcatcgtgct gcgcgaccgc gagcgcggcg gagccgagcg gcgcgtagac
     1861 cgcgacacgc tggtgtacga ggtcaagcgc ctgcagcagc gcaaccgcga gcagccggtg
     1921 gtgatcgccg ccgacgagaa cgtgccctac aagcacgtgg tgcaggtgat ggatctgctg
     1981 caggcgaacg gcgtggcgcg ggtcgggctg ctgacccgtc ccctgtccgg ctgagcgggg
     2041 agcgtgttgc tcgacggcgc gcgcgagccc ggtcggagca cctcgctgct gctggcggtc
     2101 gcggtgcatg tgctgttctt cgcgttcctg tacttcggcg tgagctggcg gcccaagccg
     2161 caacctccgc tcagcgccga gctgtggagc gagctgccgc cggtgcgccc ggtgaaggcg
     2221 cgccccgagc ctaggatcga gcccccgccg cccaaacccg cacccaaggc agaaccgccg
     2281 ccgcccgcgg cgcagccgaa gccgccgccc gcgccgacca aggcggacat cgagctgaag
     2341 gagaagaagc aacaggaaaa gaaagccgcg gaggaggcgc gccgcaggca ggcgctcgaa
     2401 cgcgaggagg aggaaaagcg gcggcgcgag gagcgcgcgc gcgcccagga ggaggagcgc
     2461 cgggccaagg aagaggagag gcagcgccag gaggcggcgc ggcgcgagga acagcagcgc
     2521 aagcgcgagg agcaggagcg tctggcggaa ctgcgccgcc aggaggaagc ccaacgcaag
     2581 gaggaggagc ggcgccagga cgaggcgcgc cgcgagcagg aggtgcggct cgagcgcgag
     2641 gccgaggcgc ggcgcagcgc gatcctcgac gagcagcaga aactggccgc tgccgcgcgc
     2701 gagcgcgccg agatcgatgc gcgaaagcgc cgcgaggccg aggaagcggc agtccgcgcg
     2761 cgcgagctgg aggcctggaa atcgcggatc cacgaccgca tcaagagcaa gctgacgctg
     2821 ccgcccggtg tgcccgacag cgcccgcgcc gagtacagca tgaccatcat cccgggcggc
     2881 gaggtgctga cggtgaaact gcgcaagagc agcggctatc cggcgtacga cgcggccatc
     2941 gagcgcgcca tcaacgccgc ggcgccgctg ccggtgccca gcgaccccaa cctgttccag
     3001 cagctgcgcg agctgaacct ggtgttccgt cccaactgag agccgctata attccgggcc
     3061 gatgaccttc ctgcgccgac ccgtcagcat cggactcgat acgcatcgcg atgtcgtctg
     3121 cggacgccgc aacgcgctca aggcgctcgg cgccgcctgt ctcggtgcgg cgacgccgac
     3181 cgcggcccag ctcaccatcg agatcgtcgg cggcggagcc gatcagatcc ccatcaccct
     3241 ggtccccttc ggaaacgagg aacgcttcgc gcagaaggtg agcgagatcg tctccgccga
     3301 tctcgcgcgc agcgggcgct tcaagctgca ggagcccggc agcgtgcggc cgctgcccac
     3361 cgacctgccc gagatcaact accgatactg gaagaaccgc ggcagtcaga ccctggtggt
     3421 gggcaaggtg ctggagcgca acgacggccg cgtggaggtg cgcttccgca tgaccgacac
     3481 cgtgaaggaa gcgcagctcg tcggctactc ttacgccatt ttgcagagcc agctgcgcgc
     3541 caccgcgcac aagatcgccg acctgatcta cgagaagctc accggcgagc ccggcgtgtt
     3601 cagcacgcgt atcgcgtacg tggcgcgcaa cccgaaccgc ttcgagctgc acgtggccga
     3661 tgccgacggc tacaactcgc agttcattct ggcgcaccgc gagccggtca tctctccggc
     3721 ctggtcgccg gacggcagcc gcatcgccta cgtgtccttc gagcagcgca agtcgatcgt
     3781 gttcctgcac gagatgagcg gacagcgccg catcctggcc aacttcgagg gcagcaacag
     3841 cgcgccggcc tggtcgccga acggcgagcg cctggcgctg gtgctgtcca aggacggtgt
     3901 cgcgcagatc tatacgctgc gcgccgcggg cggcggcgag cctgaccgcc tcaccttcag
     3961 caatgccatc gacaccgagc ccagcttttc gccggacggc aagtggctgc tgttcacctc
     4021 cgaccgcggc ggcagtccgc agatctaccg gatgcccgcg ggcggcggca atgccgagcg
     4081 catcaccttc gagggcagct acaacgtcac cccgcgctac agccccgatg gcaagtcctt
     4141 cgccttcatc cagcgcagcc agggccgcta cagcctggct ctgatggatc tcgccgcccg
     4201 tcagatccag atcctcaccg acggccagct cgacggctcg cctacgttcg cacccaacgg
     4261 ccggctggtc atgtatgcat ccatcgtgaa gggccgtggt atattagcgg ccgtctcgag
     4321 cgacggccgg atcaagcaga agatcacggc ctcggccctt gacgtacgcg aacccgcgtg
     4381 ggggccgctg gcgggaaacc gcacctagca cgaaccatcg tgtcacgtcc cgccggcgca
     4441 ttgcgcgcac cccgctcaca accggcccgg aagccgcctt gttcatcctc gccaccgttc
     4501 gccgcgcttg caaagcgggg cgagcggatc gtgcgcgaac tgcccaacga cttcgacccg
     4561 aggagcctcg catgaagaag acattgatcg gcctgtgcgt cgcgctgctg ctcgccggat
     4621 gcggcggcag aggcggagag aagccaccgg cctccgtgga gcggcgcgag gtgtccgatc
     4681 aggccagatc ggacgcggcc gtgcaggccg ccgccgagga ggcgcgccgc aagcaggccg
     4741 aggaggacgc gcgccgcctc gcggccgagg aagaacggcg ccgcgccgaa gaagccgcgc
     4801 gcaaggcggc ggccgagcag ccggtggtgc agccgctgcc cgaagcgccg gtggccgcgc
     4861 ggccgctcgg cgatccggcc tcggtgctgg ccgatccggc cagtctgctg tccaagcgca
     4921 gcgtgtacta cgacttcgac atgtacaaca tccgcgagga attccagccc ctggtcgagg
     4981 cgcacgcgaa gttcctgcgc gagaaccggg acctgcgcgt gcgcgtcgag ggcaattgcg
     5041 acgagcgcgg cagccgcgag tacaacctcg cgctcgggca gcggcgcgcg gattcgatca
     5101 agcgcgccat gacgctgctg ggcgtgccgg cgcggcagat cgagacggtc agcttcggcg
     5161 ccgagaagcc gaaggcgatc ggcagcaacg aggaagcctg ggcggagaac cgccgcagcg
     5221 acatcatcta cgttggcatc gattcgcagc agtaggcggt tgctctcccg ccgcggcggg
     5281 ggagcggggg ccgccggccg caccatgctg gccgcgtgtg cgctcatcgc ggcgacgccg
     5341 tcggccgtgc tcgcccagca gcccggccag cagaaccgca acgtcatcga gctgatgaat
     5401 cagatccagg cgctgggcgc cgacctgaac cgcttgcgcg gcgagctcga ggtgctcaac
     5461 cacagcctgg agaacgcgca gcggcgccag cgcgacatgt acctcgacct cgacacccgg
     5521 ctgcggcggc tggagggtac ggggccggag gcggcccaga agcaggacaa ggccctggcg
     5581 gatctcgaag cgcgcttgaa acgcctggag gaagcggtcg cggcgccggc agcggcggac
     5641 gagctggaag cacgcgtcaa gcgtctcgag gcgcagattg cccccgccgg cgcggcggcg
     5701 cccgccactt cctccggcgg cgccgcagcc gcttccgctg cgccggccac gccgcgccct
     5761 gccgcgccgg cgagcgaggc ctcgccgagc aagcgtgcct acgacagcgc gctgtccgcg
     5821 taccgcgcag gcgactacca gggtgcgatc gccgccttcg acgccttcgt caagcgctac
     5881 ccacgcgacc cgctggcgcc caacgcccag tactggatcg gcgacgcgtg gttcaatctg
     5941 cgggacttcc gcgccgcggc caatgcgcag caggcgctga tcagcaacta tccagacagc
     6001 ccgaaggtcc ccgacgcgat gctcaacctg tcgagctcgc agatcgcgct cggcgacagc
     6061 ggcgccgcgc gcaagacgct cgaggagctg gtgaaccgcc acccgcagag cgacgccgcc
     6121 gaacgggctc gccagcgtct gcccaagctg cgctgaacgg cgcacgcgtt ctattccgcg
     6181 gggaacgacg ctacggcgcc gggtgtagaa tcaggctttc ttccgaagcc gcggtcccgt
     6241 gctcgacccc gctcatcccg ttctcgatcc ggagcatctg ggcgcgacca tcgcatggct
     6301 gggcttcggt ctcgcgttcg tcttcggtgc ggtggcccat cgcgtgaact tctgcaccat
     6361 gggcgcggtc tccgacgcgg tcaacatcgg cgactggggg cgcatgcgca tgtggctgct
     6421 cgccatcgcc atggcgatgc tcgccgccaa tctgctgcac ctcgccggat acgtcgacct
     6481 ggagcgctcc atctatccgg ccgagcgctt ctcctggttg tcctacctcc tcggcgggtt
     6541 cctgttcggc gtggggatga cgctggcgag cggctgcggc agccgtaacc tggtgcgtct
     6601 cggcggcggc aacctgaagt cgctggtggt gctgacgttc ctcgccatgt ccgcctacat
     6661 gaccatgaag gggctgctcg ccgtgccgcg cgccggtgtg ctcgatccgg tggcgctgca
     6721 cttcgacggc ggacaaggcc tgcccaatct gctcgcgggt ctggcgggga tggacggccg
     6781 cgctgcgcgc tggatcgcga tgggatccgt ggtcgccgcg ctgctcgtgt tcgtgctcaa
     6841 ggatcgcgag ttccggcgca gcagggagtt gatcctgggc ggcgtggtgc tcgggctcat
     6901 catcgccgcc gggtggtacg tgaccggcta cctcggctac gttcccgagg acccgcagac
     6961 actcgaggaa actttcgccg gtaccaacac ccgccggccg gaatccttca gcttcgtcgg
     7021 gccggtgggc tacagcctgg agctgctgct gctgtggacc gacagttcgc tgcgcgtcac
     7081 cttcggcgtg gcggtcgtgg ccggcctgct gctcggctcg ctcggctatg cgctcgccac
     7141 gcgcaacttc cgctgggaga ccttcgcctc ggtggcggat ctgcgcaacc acatgctggg
     7201 cggagtgctg atgggctttg gtggggtcag cgccatgggc tgcaccatcg ggcagggctt
     7261 gagcggcgtg tccacgctcg cgctcggctc gtttctcgcg ctcgccgcga tcgtcgcggg
     7321 cagcgcggtc acgatgaagt tcctctattg gcggcttgcc gcgaaaggct gatcggcagc
     7381 ggcacgccct gcgctctggt ttgacacccc cgttggtttg acaccctagg ggggcgctcc
     7441 ttacaatccg cgtttctttt ttttccgggc ggttagctca gccggtagag cagcggactt
     7501 ttaatccgtt ggtcgggagt tcgaatctcc caccgcctac cacgtcctcc gcccgcgttg
     7561 cctcctccgc cccggcgcgg cggcgccagc cgcacacgcc gctggtatca ttcgacacgt
     7621 cgtcatccgg tatccgtgca agggggccgt tagctcagct ggtagagcag tggactctta
     7681 atccattggt cgtaggttcg aatcctacac ggcctaccag atgcgccctt aaggcgcgac
     7741 ttaaaaggcg ttccgcccgc cacgtgcagc atgcgcgcag gcggcaggca gtgctagccc
     7801 gccagccgct tcgccaggtc gccgagcttc gcgatggcga gttccagcct cggggtcagc
     7861 gcgtgcccgc agttgaggcg gatgaagttg cggtacttgc cgctgttggt gaacatcgga
     7921 ccgggtgcga tggcgatgtg cgcgtcgcgc gccagctcga acagccgcac accatcgacc
     7981 tgcggcggca gctcgaccca caacacgaat ccgcccgcgg gctgagtgat gcggcagccg
     8041 gcgggaaagt gctcggccac cgcctcggtg acctgcagca cttgccgcag gaatgcggcg
     8101 cgcagcctgc gcaggtgatg gtcgtagccg ccgttggcga tgaactgcgc gatggccatc
     8161 tgcggcagct ccggggtggc gatggagctg atgaacttga gcagttccac ctccttgcgg
     8221 aagcggccgg gggcgatcca gccgatgcga aaccccggcg ccaggctctt ggtgaaggag
     8281 ctgcacagca acacgttgcc ggccccgtcc caggccttgg cgacgagcgg gcgcgagcgg
     8341 ccgaagtagc actcgccgta gatgtcgtcc tcgatcagcg gaatgccgcg ggcctgcagc
     8401 agccgcacca gacgctcctt cttctcgtcc ggcatcaggc tgccgagcgg gttgctgtaa
     8461 ttggtgatca gcatcaccgc cttgaccgtg cccggccgct gggtggcgag gtcgagcgcc
     8521 tccagcgaaa tgccttcacg cggatgcgtc ggcacctcga gcgccttcat tccgaggctc
     8581 tcgatgatct gcagcagggt gaagtaagtg ggcgactcga gcgcgatcgt gtcgccaggc
     8641 ttcgccaccg cgcgcagagc cacgttgagg gcctcggtgc agccgttggt gacgacgatg
     8701 tcttccgggt gcaaggtgca gccgaattcg acggcgcgcc gggcgatggc ggctttcagc
     8761 tgccagttcc cggtggacat cacgtagcgc gacgccagat ccgggcgccg ccgcccgatc
     8821 gcgccgagca gccgctgcag cttctccccg ggatacagct cctcgctcgg cgtcgcgcag
     8881 ccgagcggcg ccacgccggg cgtctccgaa caggccaggt actccatcac ggtgcgattc
     8941 acgccgacga ggcgcgccgc ggcgggcggc gaggacgggg cgggctcggc gagcttgcgc
     9001 ccgcgcgaca acacgaagta accggacttc gggcgggcct cgatcagccg ctggctctcc
     9061 agccagcggt aggcctgtac cgcggtggcg atgctcaccc gatgctgggt ggagagctgc
     9121 cgcaccgacg gcagccgatc gccggccttc acgctgccgg cgtggatcgc gtcggacagg
     9181 cgatgggcaa gctcctgata gaggtggggc gagcggtcgt ccatggcgtc catcctggtt
     9241 cagatcggcc cggcgcgaca gccacagaga ccggcgagcg cagccatgac agatatcggt
     9301 gtcccgcatc tgtatcggct caaattgtag tccggtatga ctgtatcggc ttgcccgcgt
     9361 cgcctacact gcccggcact ggcatcgcgc cggggccaac cgatgtgggt cgaaccgatt
     9421 atttcgttga ctgcagagcg gtctgtgcgc atccgcgcca aggtgcgcac gcgcatcggc
     9481 tgcgagtccg gtctggtgtg gctcactcga gcaggggatc cgcgcgatct gttcctcgcc
     9541 cgcggcgagt tcatcgaact ggcgcccggg cccggggtga tcatcgcgac cgccctggaa
     9601 gcgaccgtga tcagcgtgac gcagcccggt tcacgctcgt ggcgcgagcg gct
//
LOCUS       DASPGE010000258         6758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_304150, whole genome shotgun sequence.
ACCESSION   DASPGE010000258 DASPGE010000000
VERSION     DASPGE010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304150"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..250)
                     /locus_tag="VD791_08395"
     CDS             complement(8..250)
                     /locus_tag="VD791_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08034.1"
                     /translation="MNRCSQRLNVMYCIHSAQAQRSCAGKPTDVTTLFPGASTALTGY
                     DGAPVHWAVCIYPEAPVAWDHGPDYAYSCKKTCVMC"
     gene            complement(455..1096)
                     /locus_tag="VD791_08400"
     CDS             complement(455..1096)
                     /locus_tag="VD791_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HXV08035.1"
                     /translation="MFDDRNDAARQLAVRLERYKGQNPLVLGVPRGAVPMACLIADAL
                     QGEVDVVLVRKLRAPGNPEFAVGAVEESGWAFVAPHAHSAGADEAYLEREKQAQLELI
                     RQRRAQYTAHRPRIDPAGRIVIVIDDGLATGSTMIAALHAMRGQHPARLICAVPVSPL
                     DTLEEVSKHADETVCLEAPLNFYAVGQFYREFHQVEDSDVVAALEQRPGRAKA"
     gene            complement(1142..1987)
                     /gene="folD"
                     /locus_tag="VD791_08405"
     CDS             complement(1142..1987)
                     /gene="folD"
                     /locus_tag="VD791_08405"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HXV08036.1"
                     /translation="MTAQLIDGAALSAQLRSALKQRAAALTASGQRPGLAVMLVGEDP
                     ASRVYVRNKIKACGEVGIHSQLYELPADAPEARVLERIRALNADPKIHGILVQLPLPP
                     QVSEEKVLDTIGVDKDVDGFHVHNLGSLMAGKDGFTPCTPAGCMAMLDHIGAPLEGRE
                     AVVVGRSNIVGKPMALMLLQRSATVTICTSRTRDLAAHTRRADVLVVATGKPRLVTGD
                     MIKPGAIVIDVGVTRTAEGKLVGDVDFASAREVAAWITPVPGGVGPMTIAMLLSNTVR
                     SAERR"
     gene            2407..3354
                     /locus_tag="VD791_08410"
     CDS             2407..3354
                     /locus_tag="VD791_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HXV08037.1"
                     /translation="MPTNLDDKLVVAISSRALFNFEEENAVFSEGDPTPYQKLQMDRL
                     DQPAKEGIAFQLVKKLLAFNSPQEQKVEVVILSRNDPVSGLRVFKSAEHHRLPITRGV
                     FLRGRSPFGYLSALKADVFLSAHQDDVAMALEAGLPAARVFPQSVEASHAHPDEVRIA
                     FDGDAVIFSDQSEIVYQEQGLAAFEAHERSRSGEPLPPGPLQPFLRALHRLQRNPPRD
                     QRMRIRTALVTARSAPAHDRAIRTLMSWEIEVDEAMFLGGLEKGPFLQVFEPDFYFDD
                     QLTHVQSAVKAAVSTGHVEAGVVNRMIRSRRPEKVQSAT"
     gene            complement(3448..3936)
                     /locus_tag="VD791_08415"
     CDS             complement(3448..3936)
                     /locus_tag="VD791_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV08038.1"
                     /translation="MRPGGRLLAVALLAAVPAALVGAGLHVDEKGFVTLEPGEEDWKD
                     SPGLPGLQYMVVEGDPKKPGPYVVRVKFSPGTMSMPHYHPEDRLATVIKGTWWTGKGE
                     TFDPKSTEPLRAGSFMKHPAGAPHFDGAKDEEVIVQLIGVGPSDTVFIKPELGRTGRS
                     ME"
     gene            complement(3946..5193)
                     /locus_tag="VD791_08420"
     CDS             complement(3946..5193)
                     /locus_tag="VD791_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXV08039.1"
                     /translation="MQRDTQLSLEIDQRAATLEQQVIGWRRHVHQHPELSNREVMTGR
                     TVAAHLETLGLQPQTQVAYTGVVALLDTGRPGPVVALRADMDALPVTEEVDVPFASKA
                     CSTCNGQEVGVMHACGHDCHVAILMGVASLLTGMKDRLRGRIKFIFQPAEEGPPAGEE
                     GGAALMIKQGVLEDPRPDVIFGLHVYAGVEAGRIAYRPGPAMASADRIRILVRGSQTH
                     AALPWRGIDPIVVSSQIIMALQTVVSRRVDVTLEPAVVSIGSIRGGVRDNIIPDEVEL
                     LGTMRTFNETMRSEIQARIRTIVDHVARSAGAHGEVFFDNPYPVTYNDESLTERMLPT
                     LKRVAGDHNVFLSPKITGYEDFSCYQQHVPGFFYFVGVTPKGTDLRKAAPNHSPRFFV
                     DESALLLGLRSLAHLTVDYLQRD"
     gene            complement(5204..6025)
                     /locus_tag="VD791_08425"
     CDS             complement(5204..6025)
                     /locus_tag="VD791_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXV08040.1"
                     /translation="MLASSLRSVALSCLLAIAPCASADIGQQLDRILAGDHRNPKNAA
                     RDQYRHPKETLMFFGIRPDMTVVEVWPSAGWWTEVLAPLLKDDGKYYAAWFATEWEKT
                     PDFLKAREKEYDAMLAARPDLYGKVVKTKLLAPQWADIAPKGSADMVLTFRNVHNWAK
                     GGYAEPMFQAFWDALKPGGVLGVKDHRANPGTSFQQQVDSGYMTEEYVISTAQKVGFK
                     LTGRSEVNANAKDTKNHPEGVWTLPPTLRLGDKDRAKYLAIGESDRMTLMFVKPK"
     gene            complement(6085..6585)
                     /locus_tag="VD791_08430"
     CDS             complement(6085..6585)
                     /locus_tag="VD791_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08041.1"
                     /translation="MPARSFRFAVLLAAALGGLSSTWAQTSPQEEIERSLRARGAAER
                     ELTMRLEVPRGPPPPVPAGEVRRAATLPTPGSEILERRPPPELPPAPAVVSPGAPAVS
                     AADLLQDSQRRRQSALQAENARLPIDDPARQQSTQIQGLTFEREMRAQDLGSAIMRSS
                     ERAVGR"
BASE COUNT         1020 a         2348 c         2301 g         1089 t
ORIGIN      
        1 ctacagatca gcacatgacg cacgtcttct tgcagctgta agcgtagtcc ggcccatgat
       61 cccaggccac cggcgcctcg ggatagatgc ataccgccca gtgcaccggc gccccgtcgt
      121 agcctgtcag agcagtggaa gcccccggaa acagggtggt cacgtcggtt ggcttgccgg
      181 cgcagctgcg ctgcgcctgc gcactgtgga tacagtacat gacgttcaac cgctggctgc
      241 aacggttcat cagcaccgcg ctcgcgcccg gcttcgactc gactctgagg caatcccgca
      301 cgccgtcggc gctgaccggc gcaacgccag gcaatagcag acagccggca gcgcacagcc
      361 aatgcacgct ccgggagcag cgcagcgagc tccggagcag tgttgccacg ggcgggatcg
      421 gcgaggcggg gttcgagcgc gacaacatcc gggtctacgc cttcgcccgc cccggccgtt
      481 gctccagcgc cgccaccacg tcgctgtcct cgacctggtg gaactcccgg tagaactgtc
      541 ccaccgcgta gaagttcagc ggcgcctcca ggcacacggt ctcgtcggcg tgcttggaaa
      601 cttcctccag cgtgtccagc ggcgacacgg gcaccgcgca gatcagcctg gccgggtgct
      661 gtccgcgcat cgcgtgcaac gccgcgatca tcgtcgagcc cgtggccagg ccgtcgtcga
      721 tcacgatgac gatccgcccc gccggatcga tgcgcgggcg atgcgccgtg tactgcgcgc
      781 ggcgctggcg gatcagctcg agctgcgcct gcttctcgcg ctccaggtag gcctcgtcgg
      841 cgcccgccga atgggcgtgc ggtgcgacga aggcccagcc gctctcctcc acggcgccca
      901 ccgcgaactc ggggttgccg ggtgcgcgca gtttgcgcac cagcaccacg tcgacctcgc
      961 cttgcagcgc atcggcgatc aggcaggcca taggcactgc gccccgcgga accccgagca
     1021 ccagcgggtt ctggcctttg tagcgttcca gcctgacggc aagctggcgc gccgcgtcgt
     1081 tacgatcgtc gaacatggct cgcgagtgtc ctgcgttccg gcgccctgtg agaaacgcgg
     1141 cctagcgccg ctccgccgag cgcacggtat tcgagagcag catcgcgatc gtcatcggcc
     1201 cgaccccgcc cggcaccggc gtgatccagg cggccacctc cctggcggag gcaaaatcga
     1261 cgtcgcccac gagcttgccc tcggcggtgc gggtgacgcc cacgtcgatc acgatcgctc
     1321 ccggcttgat catgtcgccg gtgaccaggc gcggcttgcc ggtggcgacc accagcacgt
     1381 cggcgcggcg cgtgtgcgcg gcaaggtcgc gcgtgcgcga agtgcagata gtcacggtgg
     1441 cgctgcgctg gagcagcatc agggccatgg gcttgcccac gatgttgctg cggcccacca
     1501 ccaccgcttc gcgtccctcc aacggcgcgc cgatgtgatc cagcatcgcc atgcagccgg
     1561 ccggtgtgca cggcgtgaac ccatccttgc ccgccatcag gctgccgagg ttgtgcacgt
     1621 gaaacccgtc cacgtccttg tccacgccga tggtgtccag cactttctct tcgctcacct
     1681 gcggcggcag gggcagttgc accaggatgc catggatctt cgggtcggcg ttcaacgccc
     1741 gaatgcgctc cagcacccgg gcctcgggcg catccgccgg cagctcgtac agctgggagt
     1801 ggatgccgac ctcaccacag gctttgatct tgttgcgcac gtatacgcgc gaggctgggt
     1861 cttcgcccac cagcatgacc gccaggcccg ggcgctggcc gctcgccgtg agcgcggctg
     1921 cgcgctgttt cagcgcgctg cgcagttgcg ccgagagcgc ggcgccgtcg atgagctgtg
     1981 ctgtcatggg cgatcgggcc gacaggtctc gcgcttcgga cgcggtcacg aggccggcgg
     2041 catgcgggga ttcagagccg tcgcagaatg gtttattcta ctcgcatcgg tcaggccgat
     2101 tgagggaggc ggcggtgaca ggcaggagcg cagaggccgg gacggcccta tgcggcatcg
     2161 ctgtattgct cggcggggac aacctgcggg ttcgacccat cgccccgccg cccccgggca
     2221 ccctgggacc acctcggcgc cggcggtgcc cggcgccgaa cgagctctat caagaccatg
     2281 ccgattgcgt ccgaaaagta accgaggccg tgcccgggcc gcagcgcggg cggcggacgt
     2341 tgccggcgga cgccgagccg acgacgatcc gcgatgccca tgaacacgaa ctacagaggt
     2401 agactcatgc cgaccaatct ggacgacaag ctcgtcgtcg ccatctcttc ccgcgcgctg
     2461 ttcaacttcg aggaggagaa cgcggtgttc agcgagggcg accccacgcc ctaccagaag
     2521 ctgcagatgg accggttgga ccagccggcc aaggagggaa tcgccttcca gctggtgaag
     2581 aagctgctcg ccttcaacag cccgcaggag cagaaggtcg aggtggtgat cctgtcgcgc
     2641 aacgacccgg tgagcgggct gcgcgtgttc aagtccgccg agcaccacag gctgcccatc
     2701 acccgcgggg tgttcctgcg cgggcgctcg ccgttcggct acctgagcgc gctgaaggcc
     2761 gacgtgttcc tgtccgcgca ccaggacgac gtcgccatgg ccctggaagc cggcctgccg
     2821 gccgcgcgcg tcttcccgca atcggtcgag gccagccacg cgcacccgga cgaggtgcgc
     2881 atcgccttcg acggcgacgc cgtgatcttc agcgaccagt ccgagatcgt gtaccaggag
     2941 cagggcctgg cggcgttcga ggcgcacgag cgctcgcgca gcggcgagcc gctgccgccc
     3001 gggccgctgc agccgttcct gcgcgcgctg catcgcctgc agagaaaccc cccgcgcgat
     3061 cagcgcatgc gcatccgcac ggcgctggtc accgcccgca gcgccccggc ccacgaccgc
     3121 gccatccgca cgctgatgag ctgggagatc gaggtggacg aggcgatgtt cctcggcggg
     3181 ctggagaagg ggccgttcct gcaggtgttc gagcccgact tctacttcga cgaccagctc
     3241 acccacgtgc aatcggcggt caaggccgcg gtgtcgaccg gacacgtgga agccggcgtg
     3301 gtcaaccgca tgatccgctc gcgccggccg gagaaagtgc agtccgcgac ctgagaaggc
     3361 tcccctcccc gtgcgggaga ggggcggggg tgagggttca gcgatggtcc gctaggcgcg
     3421 ctgagcggat gcagcgggcg accgccttca ctccatcgac ctgccggtcc gtccgagctc
     3481 cggcttgatg aagacggtgt cgctcgggcc cacgccgatc agctgcacga tcacctcctc
     3541 gtccttggcg ccgtcgaagt gcggcgcccc ggcagggtgc ttcatgaagc tgccggcccg
     3601 cagcggctcg gtgctcttgg gatcgaaggt ctcgcccttg ccggtccacc aggtgccctt
     3661 gatcacggtg gccagccgat cctccgggtg gtaatgcggc atgctcatgg tcccgggaga
     3721 gaacttcacg cgcaccacgt agggaccggg cttcttggga tcgccctcca ccaccatgta
     3781 ctggaggccg ggcaagccgg gagagtcctt ccagtcttct tcgccgggct cgagcgtgac
     3841 gaagcctttc tcgtccacgt gcagcccggc cccgaccagt gccgcgggca ccgcggcgag
     3901 caacgcaaca gcgagcagcc gtcctccggg tcgcatgcag tctcctcagt cccgttgcag
     3961 gtagtcgacg gtcaggtgcg ccagtgagcg caggccgagc aagagcgccg attcgtccac
     4021 gaagaagcgc ggtgagtggt tgggcgcggc cttcctcaga tccgtgccct tcggtgtgac
     4081 gccgacgaag tagaagaagc cgggcacgtg ctgctggtag caggagaaat cctcgtagcc
     4141 ggtgatcttc gggctcagga agacgttgtg gtctcccgcc acgcgcttca gcgtcggcag
     4201 catccgctcg gtcagcgact cgtcgttgta ggtgaccgga tacgggttgt cgaagaacac
     4261 ttcgccgtgg gcgccggcgc tcctggcgac gtgatcgacg atggtgcgga tgcgcgcctg
     4321 gatctcgctg cgcatggtct cgttgaaggt acgcatggtg cccagcagct cgacttcgtc
     4381 ggggatgatg ttgtcgcgca cgccgccgcg gatgctgccg atgctcacca ccgcgggctc
     4441 gagcgtgacg tccacgcggc ggctgacgac ggtctgcagc gccatgatga tctgcgagga
     4501 caccacgatc gggtcgatgc cgcgccacgg cagcgcggcg tgggtctgac tgccgcgcac
     4561 caggatgcgg atgcgatcgg cgctcgccat ggcgggaccc ggccggtagg cgatccggcc
     4621 cgcctcgacg ccggcgtaga cgtgcaggcc gaagatgacg tccgggcgcg ggtcttccag
     4681 caccccctgc ttgatcatca gcgcggcgcc gccttcctcg ccggccggcg ggccctcttc
     4741 cgccggctgg aagatgaact tgatcctgcc gcgcagccga tccttcatcc cggtcagcaa
     4801 cgacgccacg cccatcagga tcgcgacgtg gcagtcgtgc ccacaggcgt gcatcacgcc
     4861 cacctcctga ccgttgcagg tggagcacgc cttcgacgcg aacggcacgt ccacctcctc
     4921 ggtgaccggc agcgcgtcca tgtcggcgcg cagcgcgacc accggccccg gtcggccggt
     4981 gtcgagcagc gccacgaccc cggtatacgc cacctgggtc tgcggctgca gcccgagcgt
     5041 ctccagatgc gctgcgaccg tacgcccggt catcacctcg cggttggaca gctccggatg
     5101 ctgatggacg tgccgccgcc agccgatgac ctgctgctcc agggtggccg cgcgctggtc
     5161 gatctccagg gacagttgcg tgtctcgttg catccgtcgc gtgtcacttc ggcttcacga
     5221 acatcagcgt catgcggtcg ctctcgccga tcgccaggta cttcgcgcga tccttgtcgc
     5281 ccaggcgcag cgtcggcggc agcgtccaca ccccctcggg gtggttcttg gtgtccttcg
     5341 cgttggcgtt cacctccgag cggccggtga gcttgaagcc gaccttctgc gcggtgctga
     5401 tcacgtactc ctcggtcatg tagccgctgt cgacctgctg ctggaacgat gtgcccgggt
     5461 tcgcacggtg atccttgacg cccagcactc caccgggctt gagcgcgtcc cagaaggcct
     5521 ggaacatggg ctcggcgtag ccacccttgg cccagttgtg cacgttgcgg aaggtcagca
     5581 ccatgtcggc cgagcccttg ggggcgatgt cggcccactg cggcgcgagc agcttggtct
     5641 tgacgacctt gccgtacagg tccgggcgcg cggcgagcat ggcgtcgtat tccttctcgc
     5701 gcgctttcag gaaatcgggg gtcttctccc actcggtggc gaaccatgcg gcgtagtact
     5761 tgccgtcgtc cttcagcagc ggcgccagga cctcggtcca ccagccggcg ctcggccaca
     5821 cttcgaccac cgtcatgtcc ggtcggatgc cgaagaacat cagggtttcc ttcgggtggc
     5881 ggtactggtc gcgcgcggcg ttcttcgggt tgcggtgatc gccggcgagg atccgatcga
     5941 gctgttgccc gatgtccgcc gaagcacacg gcgcgatggc cagcaggcag ctcagtgcca
     6001 ccgaccggag cgaggatgca agcatggatc tcctcctgga gttgaatgta cgacctgttc
     6061 tgcttcttgg gctcggggcg cggttcagcg acccacggcc cgttcggagc tgcgcattat
     6121 cgccgaaccc aggtcctgcg cgcgcatctc gcgctcgaag gtcagcccct ggatctgggt
     6181 cgactgctgg cgtgcgggat cgtcgatcgg aagccgcgca ttctccgcct gcagcgccga
     6241 ctgccggcgc cgctggctgt cctgcagcag atcggcggcg ctcaccgccg gcgcgcccgg
     6301 ggatacgacc gcgggcgcgg gcggcagttc cggcggcggc cgccgctcca ggatctcgct
     6361 gcccggagtg ggcagcgtgg ccgcgcgccg cacctcgcct gccggaacgg gcggtggcgg
     6421 tccgcgcggc acctcgaggc gcatggtcag ctcccgctcg gcggccccgc gcgcgcgcag
     6481 gctgcgttcg atctcctcct gcgggctggt ctgcgcccag gtggaggaca ggccgcccag
     6541 cgcagcggcg aggagcacgg cgaagcggaa ggagcgagcg ggcatggtgt caccagtgga
     6601 tcggaaacac ccggggaatc ggaagcaccc cgggggatcg gaaacaccgg aggtcggaaa
     6661 cgccgggagg tcggaaacga gtgtacgttc gaccgtggcc ggcgtccatg gttgcagggc
     6721 gcgagggaag ccgatcgccc gcagtcgtcc taccgacg
//
LOCUS       DASPGE010000259         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_888676, whole genome shotgun sequence.
ACCESSION   DASPGE010000259 DASPGE010000000
VERSION     DASPGE010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888676"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VD791_08435"
     CDS             <1..265
                     /locus_tag="VD791_08435"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HXV08042.1"
                     /translation="VMRRLRNWLVVERSVRLTGDDYEASVRMRLDAAQLPRPLQVSAL
                     TNRELRLESAWKTIIFQPSVAALPANAAAPSAGIRDAPVSAAR"
     gene            262..>2089
                     /locus_tag="VD791_08440"
     CDS             262..>2089
                     /locus_tag="VD791_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HXV08043.1"
                     /translation="MRVLLIVGAALAGVLLFLLATASAADTTLLARHFPLLLGLNAAL
                     AGALAALLGYQVAGILRRYRARAFGSRLTLRFLLLFALLAVAPGVLVYTVSVQFLTRS
                     IESWFDVKVDAALEGGINLGQSVIDRMMAEMQTKARAIALELADRSPTQQVSMLNRLR
                     EQAGVQEVVLLGAGGAILASATEDVTRFVPELPTASMLRQARSAQGYSSIDALDGRPL
                     ALRVVVPVTGLAIADEARFLQLRQSVPSGLARSAQAVEEAYRDYRELALSRQGLRRIY
                     IVTLTLALLMALFVAVSLAAILSARLSAPLANLAQATQAVARGDFSQRPPVTSRDELG
                     VLTESFNSMTRQLDEARKVVESNRVALETAKSSLENILANLSAGVLVFDRELRLTISN
                     RGAAQILTGELGALLGRPLDAWGGPLDSFAASVREQFLQHRERTWQLELTLKGTGKTL
                     LARGAALPGATGGGHVLVFDDITQLIQAQRATAWAEVARRLAHEIKNPLTPIQLSAER
                     LEMKLGDRLAAEDADILRRATQTIVNQVTALKSMVDDFSDYARLPAPVLTGLDLNALI
                     ADVLTLYETSRVPITRRLAPSLPPVWADGAQIRQVIHNLVQNA"
BASE COUNT          293 a          730 c          735 g          331 t
ORIGIN      
        1 cgtcatgcgt cgcctgcgca actggctggt cgtggagcgc tccgtgcggc tgaccggaga
       61 cgactacgag gcctcggtgc gcatgcggct ggacgcggca cagctgccca ggcccctgca
      121 ggtgagcgcg ctcaccaatc gcgagctgcg gctcgaatcc gcgtggaaga ccatcatctt
      181 ccagccatcg gtcgcggcat tgcctgcgaa tgcggccgcg ccgtcggccg gaatccgtga
      241 cgcaccggtg agcgccgcgc gatgagagtg ctgctcatcg tcggcgccgc gctcgcgggc
      301 gtgctgttgt tcctcctggc gacggcgagc gcggccgata ccacgctgct ggcgcggcac
      361 tttccgctgc ttctcggcct gaacgccgcc ctcgcggggg cgctcgcggc cctgctcggc
      421 tatcaggtgg cgggaatcct gcggcgctac cgcgcgcgcg ccttcggctc gcggctgacg
      481 ttgcgcttcc tgctgctctt cgcgctcctg gccgtggcgc cgggtgtgct ggtctacacg
      541 gtgtcggtac agttcctcac gcgcagcatc gagtcctggt tcgacgtcaa ggtggatgcg
      601 gcgctcgaag gcggcatcaa tctcggacaa tcggtgatcg atcggatgat ggcagagatg
      661 cagaccaagg cacgcgccat cgcgctcgag ctggccgacc gttcgccgac gcaacaggtg
      721 tcgatgctca accggttgcg cgaacaagcc ggcgtgcagg aagtcgttct gctgggcgcg
      781 ggcggggcga tcctggcgag cgccaccgag gacgtgaccc gcttcgtccc ggagctgccg
      841 accgcgagca tgctgcggca ggcgcgctcg gcgcagggct acagctcgat cgacgcgctg
      901 gatggcaggc cactcgcctt gcgcgtggtc gtgccggtga ccggcctggc gatcgccgac
      961 gaggcgcgct ttctgcagct gcgccagtcg gtgccctcgg gtctggcgcg cagcgcgcag
     1021 gcggtggagg aagcgtaccg ggactaccgg gagctcgccc tgtcgcgcca gggcctgcgg
     1081 cgcatctaca tcgtgacact gaccctggcg ttgctgatgg cgctgttcgt cgccgtatcg
     1141 ctcgcggcga tcctgtccgc gcgcctgtcc gcgccgctcg ccaatctggc gcaggccacg
     1201 caggcggtgg cgcgcggcga cttctcgcag cgtccgcccg tgaccagccg ggacgagctg
     1261 ggcgtgctga ccgagtcctt caattccatg acgcgccagc tggacgaagc gcgcaaggtg
     1321 gtggaatcca accgcgtcgc gctggaaacg gccaagtcgt cgctggagaa catcctcgcc
     1381 aacctgtcgg cgggcgtgct ggtgttcgat cgcgagttgc ggctcaccat ttccaaccgc
     1441 ggcgcggcgc agatcctgac cggcgagctg ggcgcgctcc tgggacggcc gctggacgcc
     1501 tggggcgggc cgctcgattc cttcgccgcc tccgtgcgcg agcagttcct gcagcaccgc
     1561 gagcgcacct ggcaactgga gctcacgctg aagggcacgg gcaagacgct gctggcgcgc
     1621 ggcgcggcgc tgcccggcgc cacgggcggc ggccacgtgc tggtgttcga cgacatcacc
     1681 cagctcatcc aggcgcagcg cgccacggcg tgggccgagg tcgcgcgccg cctggcgcac
     1741 gagatcaaga atcccctcac gccgatccag ctctcggccg agaggctgga gatgaagctc
     1801 ggcgaccggc tcgcggccga ggacgccgac atcctcaggc gcgccacgca gaccatcgtc
     1861 aaccaggtga cggcgctcaa atccatggtc gatgacttca gcgactacgc gcgcctgccc
     1921 gcgccggtgc tgacgggcct cgacctcaat gcgctgatcg ccgacgtgct gaccctgtac
     1981 gagacgtcgc gggtgccgat tacccggcgc cttgcgccat cgctgccgcc cgtatgggcc
     2041 gatggcgcgc agatacgcca ggtgatccat aacctggtgc agaacgccc
//
LOCUS       DASPGE010000260         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1123098, whole genome shotgun sequence.
ACCESSION   DASPGE010000260 DASPGE010000000
VERSION     DASPGE010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123098"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..378
                     /locus_tag="VD791_08445"
     CDS             40..378
                     /locus_tag="VD791_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HXV08044.1"
                     /translation="MNRRGSAAEDLALRFLVAQGLRLRKRNYACRMGEIDLVLQDGDT
                     LVFVEVRQRRSRAYGGAAESITAGKRARLLAAARHFLGRERQLPACRFDAVLVDGADR
                     VTWIRDAFGE"
     gene            458..1069
                     /locus_tag="VD791_08450"
     CDS             458..1069
                     /locus_tag="VD791_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HXV08045.1"
                     /translation="MSRVSPAVDLTARITRHFEESARVKMEAVRTLADPLKQAAERMV
                     QCLMGEGKIMACGNGGSAADAQHFAAELLNRFEMDRPGLAALALTTDSSTLTSIANDF
                     DFEQVFSKQVRALGQPNDLLLAISTSGNSRNVIEAIQAAHDRQVGVVALTGRNGGMIG
                     ELLNPGDIHLCVPHANTARIQEVHILCLHCLCDAIDCLLLGVE"
     gene            1093..1656
                     /locus_tag="VD791_08455"
     CDS             1093..1656
                     /locus_tag="VD791_08455"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HXV08046.1"
                     /translation="MIGLLVLVPLLHGCFVAAAAGVGATALMAEDRRSSGIYLEDQNI
                     EVKLASAVREKYGQDDKVQIKGTSFNRFVLLNGEVPSEEIKKDVGVMALGVESVRNVQ
                     NEIVVAPPASLTQSSSDTLTTSKVKSRFVTENRFQPNHVKVVTRGDTVYLMGLVTAKE
                     ADDATEIARTTGGVQKVVRVFEITENP"
     gene            complement(1640..>2212)
                     /locus_tag="VD791_08460"
     CDS             complement(1640..>2212)
                     /locus_tag="VD791_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HXV08047.1"
                     /translation="HHPHVVEHAFRRELDAIRFGNDEARWRAWCEGRTGYPLVDAGMR
                     QLNRTGYMHNRLRMLTASFLVKDLQVDWRRGERYFARQLNDYDLAANNGGWQWAASTG
                     CDAQPWFRIFNPVLQSQRFDPQGRFIRLYVPELARVPERFVHAPWTMSEAQQAQAGVR
                     IGRDYPAPIVDHAQARQAALEMYGRVRGSR"
BASE COUNT          386 a          713 c          769 g          344 t
ORIGIN      
        1 ggtggcgcgc cttgctgaga taaacggcag ggactcgaga tgaaccgcag gggcagcgcg
       61 gccgaagacc tggcgctgcg cttcctggtg gcgcagggct tgcggttgcg caagcgcaac
      121 tacgcgtgtc gcatgggaga gatcgacctg gtgctgcagg acggcgacac gctggtgttc
      181 gtggaggtga ggcagcgtcg cagccgggcg tacggcgggg cggcggagag catcactgcc
      241 ggcaagcgcg ccaggctgct cgctgccgcg cgccattttc tcggccgcga gcggcagctg
      301 ccggcgtgcc gcttcgacgc cgtgctggtg gacggcgcgg accgcgtgac atggatccgc
      361 gacgcgttcg gcgagtagtc cgccgtgcgg gcgtcgccgc atgacgaccg ggtgcagccg
      421 gatacgacgg cggtttatgc aataatttcg ggccatcatg agcagggtta gtcccgccgt
      481 ggacctgacc gcacgcatca cccggcactt cgaggaaagc gcgcgggtga agatggaagc
      541 ggtgcgcacg ctcgccgatc cgctcaagca ggcggccgag cgcatggtgc agtgcctgat
      601 gggcgagggc aagatcatgg cctgcggcaa cggcggctcc gccgccgacg cgcagcactt
      661 cgccgccgag ctgctcaacc gcttcgagat ggaccgcccg ggcctggccg ccttagcgct
      721 gaccaccgac tcgtccacct tgacgtcgat cgccaacgac ttcgacttcg agcaggtgtt
      781 ctccaagcag gtgcgcgcgc tggggcagcc caacgacttg ctgctggcga tctccaccag
      841 cggcaactcg cgcaacgtca tcgaggcgat ccaggccgcg cacgaccgcc aggtgggcgt
      901 ggtggcgctc accggcagga acggaggcat gatcggggaa ctcctcaacc ccggcgacat
      961 ccatctgtgt gttccgcacg caaataccgc ccgcattcag gaagtgcata tcctctgcct
     1021 gcactgcctg tgcgatgcaa tcgactgctt gttactggga gtggagtgac tatgcataca
     1081 cgtgaaactg ctttgatcgg cctgttggtc ctggttcccc tgctgcacgg ctgtttcgtg
     1141 gccgcggcgg ccggcgtcgg cgccaccgcc ctgatggcgg aggaccggcg cagcagcggc
     1201 atctacctgg aggaccagaa catcgaggtg aagctcgcca gcgccgtccg ggagaagtac
     1261 ggccaggacg ataaggtgca gatcaagggc accagcttca accgcttcgt gctgctgaac
     1321 ggcgaagtgc cgagcgagga gatcaagaag gacgtgggcg tgatggcgct cggcgtggaa
     1381 agcgtgcgca acgtgcagaa cgagatcgtg gtcgcccccc cggcgagcct gacgcagagc
     1441 tccagcgaca cgttgaccac ctccaaggtg aagagccgtt tcgtgaccga gaaccggttc
     1501 caacccaatc acgtaaaagt ggtcacgcgc ggcgatacgg tctacctgat gggcttggtc
     1561 accgccaagg aagccgacga tgccaccgag atcgcacgca ccaccggagg cgtgcagaaa
     1621 gtggtgcggg tgttcgagat caccgagaac ccctgacccg gccgtacatc tccagcgccg
     1681 cctggcgcgc ctgggcgtgg tccacgatgg gcgcggggta gtcgcgcccg atgcgcacgc
     1741 cggcctgcgc ctgctgcgcc tcgctcatgg tccagggcgc gtggacgaag cgctcgggca
     1801 cgcgcgccag ctccggcacg tacaagcgga tgaacctgcc ctgcgggtcg aagcgctgcg
     1861 actgcagcac gggattgaag atgcggaacc agggctgcgc atcgcacccg gtggaagcgg
     1921 cccactgcca gccgccgttg ttcgccgcca ggtcgtagtc gttgagctgt cgcgcgaagt
     1981 agcgctcccc gcggcgccaa tccacctgca ggtccttcac caggaatgaa gcggtcagca
     2041 tgcgcagccg gttgtgcatg tagccggtgc gattgagctg gcgcatgccc gcatcgacca
     2101 gcggatagcc ggtcctgccc tcgcaccacg cccgccagcg cgcctcgtcg ttgccgaagc
     2161 ggatggcgtc gagttcgcgc cgaaatgcgt gctcgaccac gtgcgggtgg tg
//
LOCUS       DASPGE010000261         6642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1240059, whole genome shotgun sequence.
ACCESSION   DASPGE010000261 DASPGE010000000
VERSION     DASPGE010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240059"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VD791_08465"
     CDS             complement(<1..893)
                     /locus_tag="VD791_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXV08048.1"
                     /translation="MNQREAAPGTADERVLEVVAGLARELHPRRVLRAVTLDMRFDRD
                     LGFDSLARVELMLRVERATGRALPEQMLALAETPRDLLDALQAAPLVVPTAAAGTVPT
                     GAAGAVAAAAGADAAEPLQASTLLEVLEWHARLHPERVHIVLGRERGDGLEEQSITHA
                     ALLQAAAAAASALQAHGLQPGDTVAIMLPTGADYFSTFFGAMLAGGIPVPLYPPLRAS
                     QLEDYLRRQSAILSNCRARILVTLGEAKAAARLLVARAPDLRRVLTADELREGGARFE
                     RPRVNAEDLAFLQYTSGSTGNP"
     gene            complement(890..1894)
                     /locus_tag="VD791_08470"
     CDS             complement(890..1894)
                     /locus_tag="VD791_08470"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HXV08049.1"
                     /translation="MDRPARLMVGGDVMLGRLVAESIAARGPEHPLAPIAEIMRAADL
                     TLVNLECAITASTRRWPGAPKAFYFGTPPEAVRTLTHAGVDLVSLANNHALDCDVQGL
                     RDTLRHLAQAGIACAGAGENAAAACAPALVERGGLRFALAAYCDHQEDFAAGTDTPGI
                     AWLHLNDEAGAVREFEAALGRIHSAGGAHWPILSLHWGPNFVFAPAERFMRLAHAAVD
                     AGFAMVFGHSAHNFQGVELYRERPILYSAGDLVDDYAVDPWFRNDRSLLFELEIAGTG
                     LQRMRLHPVVIDGFQTRPASGADFEWIVQRLTDQCAGFRTRVQRTRHEVWIEPGPGAT
                     "
     gene            complement(1895..2440)
                     /locus_tag="VD791_08475"
     CDS             complement(1895..2440)
                     /locus_tag="VD791_08475"
                     /inference="COORDINATES: protein motif:HMM:NF014923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HXV08050.1"
                     /translation="MKDWNVIVTVVPGPHHAHDLLGALSGRGRFAFSDFKDVLLGQVA
                     DVPAFLAGLLEAGAGGARWVANLARVIPVEQRFHFSPESLEQQLKQAAAALLEHVPAG
                     GSFHVRLERRGLAGQVATQEIERAVAEHLFELAAARGIALRTDFQDPDFVVAAETVGE
                     ECGVALVTRELRSRYPFVRVH"
     gene            complement(2542..3522)
                     /locus_tag="VD791_08480"
     CDS             complement(2542..3522)
                     /locus_tag="VD791_08480"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV08051.1"
                     /translation="MMGSRGAAVLLAALGCCAAQADERLTVVLNWVPNAEHAPLFHAH
                     QQGWYAQTGIAVEIEPVLGSPVAIERAMRGVHTLAVADFVAWLRASTAGAQGAAVMVL
                     EHGSPYAFYFDRRTGLAGPGDFTGRRLAAQPQDPMRALWPVLAARHLHDDAPDFAGSD
                     TTAVRWVDMGNAAKPEALARGDIDVALNPFLHNHLAYAAALGKELGAAWWRDLGFEAY
                     GLVLISGAALMRDEPGLLRRFVAVTQRAWAQCRAAPAPCIDALIAAQPELDRADSTAL
                     WQLAQPGPAPTGDAFDSARVERTRTDVEAAFGVRAPQAREAATNRFLDRR"
     gene            complement(3556..4947)
                     /locus_tag="VD791_08485"
     CDS             complement(3556..4947)
                     /locus_tag="VD791_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXV08052.1"
                     /translation="MSLAAADVQTAIDELRALLGERLSTSAGVRDHHSKDESWHIPHR
                     PDAVAFPASTEEVSQIVQICARHRTPVVAYGTGTSLEGAVIPKSGGVVVDLVNMDQVL
                     RVNVEDLDATVQARVTRKQLNEHIRDTGLFFPIDPGADASIGGMAATRASGTNAVRYG
                     TMRENVLGLTVVLADGRIIRTSRRARKSAAGYDLTRLFVGSEGTLGIITEVTVRLYGI
                     PEAMAAAVCSFDGLKGAVDTVIQTIQLGIPVARIELLDDVEMDAVNRFSKLDYPVKDT
                     LFLEFHGTAAGVKEQAETVQAIASENGGGEFKWATRQEERTQLWQARHDVAYATKALR
                     PGAELWSTDVCVPISRLAQCILETKADLKASFLIAPMVGHVGDGNFHCAFLIDRNNPD
                     ELKEAERLNERLVLRALAMDGTCTGEHGIGLGKMKFLTAEHGEGVAVMREIKKALDPL
                     NILNPGKIFSMRP"
     gene            5382..6428
                     /locus_tag="VD791_08490"
     CDS             5382..6428
                     /locus_tag="VD791_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HXV08053.1"
                     /translation="MRVVNVGLVGYGFAGKVFHAPLIGAVEGLRLTHVASSDAAKVQR
                     DLPQAQVVANVQALLAAPEIDLVVIATPNLSHFALAQQALVAGKHVVVDKPFVVHTPE
                     GEELARLAAARGKVLSVFHNRRWDNDFLTLREILRSGVLGAVHSYEAHFDRFRPQVRE
                     ERWREQDQPGAGVLYDLGSHLIDQALCLFGRPQTVWAQTAVQRPGGKSVDCFRLVLGY
                     RDRTAVLRAASLVRNPGPRYQVHGTRGSFIKHGLDPQEDALRQGARPGAPGWGQEAAA
                     LFGELAFDHGTLRINGRVETLPGCYQAYYGGLHAAIAEGKAPPVPAREAIDVIRVIEA
                     AMKSANLGATIDLA"
BASE COUNT          960 a         2334 c         2388 g          960 t
ORIGIN      
        1 gggttgccgg tgctgcccga ggtgtactgc aggaaggcga gatcctccgc gttcacgcgt
       61 gggcgctcga agcgggctcc gccctcgcgc agctcgtccg cggtgaggac gcgccgcaag
      121 tccggtgcgc gcgcgacgag caaccgcgca gcggctttcg cctcgccgag cgtcaccagg
      181 atgcgcgcgc ggcagttgga caggatggcg ctctggcgtc gcaggtagtc ctcgagctgc
      241 gaggcgcgca gcggcggata cagcggcacc gggatgcccc cggccagcat ggcgccgaag
      301 aaggtcgaga agtaatcggc gccggtgggc agcatgatcg cgaccgtgtc gccgggctgc
      361 aggccgtgcg cctgcagcgc acttgcggcc gcggctgcgg cctgcagcag cgcggcgtgg
      421 gtgatgctct gctcctccag cccgtcgccg cgctcgcgcc cgaggacgat gtgcacgcgc
      481 tcggggtgca ggcgcgcatg ccactcgagc acctcgagca gagtgctggc ctgcaacggc
      541 tcggccgcat cggcgccggc ggccgccgcc acggcgccag cagctccagt cggcacagta
      601 cccgcagctg cagtcggcac aacaagtggc gcggcctgca acgcatccag cagatcccgc
      661 ggcgtctccg cgagcgccag catctgctcc ggcaaggcgc ggccggtcgc tcgctcgacg
      721 cgcagcatca gctcgacgcg ggccaggctg tcgaagccga gatcgcggtc gaagcgcata
      781 tccagcgtca cggcgcgcag gacgcggcgc gggtgcagct cgcgcgcgag gcccgcgacc
      841 acctcgagca cacgctcgtc cgccgtgccg ggagcggctt cgcgctggtt catgtcgcgc
      901 ctggtccggg ctcgatccag acctcgtgcc gagtgcgctg cacgcgcgtg cggaacccgg
      961 cgcattgatc ggtcagccgc tgcacgatcc actcgaagtc cgcgcccgag gccgggcggg
     1021 tctggaagcc atcgatcacc accggatgca ggcgcatgcg ctgcaggcca gtgcccgcga
     1081 tctccagctc gaacagcagc gagcggtcgt tgcgaaacca gggatccacg gcgtagtcgt
     1141 ccaccaggtc gcccgcggag tacaggatgg ggcgctcgcg gtacagctcg acgccctgga
     1201 aattgtgcgc gctgtggccg aacaccatgg cgaagccggc gtccacggcg gcgtgcgcca
     1261 gtcgcatgaa acgctccgcc ggagcgaaca cgaaattcgg tccccagtgc agcgacagga
     1321 tcggccagtg cgcgccgccg gcggagtgga tacggccgag cgccgcttcg aactcacgca
     1381 cggcgccggc ctcgtcgttc aggtgcagcc aggcgatgcc cggcgtgtcc gtcccggcgg
     1441 cgaagtcttc ctggtggtcg cagtacgcgg ccagcgcgaa gcgcagcccg ccgcgctcga
     1501 ccagcgccgg cgcgcacgcc gcggcggcgt tctcgcccgc accggcgcag gcgatgccgg
     1561 cctgcgccag gtggcgcagg gtgtcgcgta agccctgcac gtcgcagtcc agggcgtggt
     1621 tgttcgccag gctgaccagg tcgacgccgg cgtgcgtcag cgtgcgcacc gcctccggcg
     1681 gcgtgccgaa atagaacgcc ttgggtgcgc ccggccagcg ccgggtagat gcggtgatgg
     1741 cgcactccag attgaccagg gtcaggtcgg cggcgcgcat gatttcggcg atcggcgcga
     1801 gcggatgctc ggggccgcgc gcggcgatgc tctccgccac caggcggccg agcatgacat
     1861 cgccgcccac catcaggcgc gccggccggt ccattcagtg cacgcgcacg aacgggtaac
     1921 ggctgcgcag ctcgcgcgtg accagcgcca cgccgcactc ttcgcccacg gtctccgccg
     1981 ccaccacgaa gtcggggtcc tggaaatcgg tgcgcagcgc gatgccgcgc gcggcggcca
     2041 gttcgaacag gtgctcggcc acggcgcgct cgatctcctg ggtggccacc tgcccggcca
     2101 gcccgcgccg ctccaggcgc acgtggaagc tgccgccggc gggaacgtgc tcgagcaggg
     2161 cggccgcggc ctgcttgagc tgctgttcga gcgactccgg ggagaagtgg aagcgctgct
     2221 ccaccgggat cacacgcgcc agattggcga cccaccgggc gccgccagcg ccggcttcga
     2281 gcagccccgc gaggaaagcc ggcacgtccg caacctggcc gaggagcacg tccttgaagt
     2341 cgctgaaggc gaagcggccg cgtccgctca gcgccccgag caggtcgtgc gcgtgatgcg
     2401 gtccggggac gacggtgacg atcacgttcc agtctttcac gggcggccca tgggtggatg
     2461 ccggcaccca ggataatgga ctgccgtcgc cgggacttga gctggcgcaa ggcgggacgc
     2521 tttcgcgtca gcagcggcgc gtcagcggcg gtcgaggaag cggttggtgg cggcctcgcg
     2581 tgcctgcggc gcgcgcacgc cgaacgctgc ctccacgtcg gtccgcgtgc gctctacccg
     2641 cgcgctgtca aatgcgtccc cggtcggggc cggcccgggc tgggccagct gccacagcgc
     2701 ggtcgaatcg gcccggtcca gctcgggttg cgccgcgatc agggcatcga tgcagggcgc
     2761 cggcgccgcc cggcattgcg cccacgcgcg ctgcgtcacc gccacgaagc ggcgcagcag
     2821 cccgggctca tcgcgcatca gcgcggcgcc cgagatcagc accaggccat aggcctcgaa
     2881 gccgaggtcg cgccaccacg ccgcgcccaa ttccttgccc agcgccgccg cgtaggcgag
     2941 gtggttgtgc aggaacgggt tgagcgcgac gtcgatgtcg ccgcgcgcga gcgcttcggg
     3001 cttggccgcg ttgcccatgt ccacccagcg caccgctgtc gtgtcgctgc cggcgaaatc
     3061 cggtgcgtca tcgtgaagat ggcgtgccgc cagcaccggc cacagcgcgc gcatcgggtc
     3121 ctgcggctgc gccgcgaggc gccggccggt gaagtcgccc ggcccggcga ggcccgtgcg
     3181 ccgatcgaag tagaacgcgt acggcgaacc gtgctcgagg accatgaccg cggctccctg
     3241 cgcgccggcg gtgctggcgc gaagccaggc cacaaagtcg gccacggcga gcgtgtgcac
     3301 gccgcgcatc gcgcgctcga tggctaccgg cgaaccgagg accggctcga tctccaccgc
     3361 gatgccggtt tgggcatacc agccctgctg gtgggcatgg aacagcggcg cgtgctccgc
     3421 gttgggtacc cagttgagca cgacggtgag gcgctcgtcg gcctgcgccg cgcagcagcc
     3481 gagcgccgcg aggagcaccg ccgcgccccg cgatcccatc atcgcatggc ccccggccgt
     3541 tttctaagac gcgccttaag ggcgcatcga gaagatcttg cccgggttca ggatgttcag
     3601 cgggtcgagc gccttcttga tctcgcgcat caccgccacg ccctcgccgt gctcggcggt
     3661 gaggaacttc atcttgccca gcccgatgcc gtgctcgccg gtgcaggtgc cgtccatcgc
     3721 cagcgcgcgc agcaccaggc gctcgttgag gcgttcggcc tctttcagct cgtcggggtt
     3781 gttgcggtcg atcaggaagg cgcagtggaa gttgccgtcg cccacgtgac ccaccatcgg
     3841 cgcgatcaga aagctcgcct tcaagtccgc cttggtctcc aggatgcact gcgcgagccg
     3901 cgagatcggc acgcacacgt cggtggacca gagctcggcc cccgggcgca gggccttggt
     3961 ggcgtaggcc acgtcgtggc gcgcctgcca cagctgggtg cgctcttcct gccgggtggc
     4021 ccacttgaac tctccgccgc cgttttccga ggcgatcgcc tgcaccgtct cggcctgttc
     4081 cttgacgccg gccgcggtgc cgtggaattc gaggaacagc gtgtccttga ccgggtagtc
     4141 gagcttcgag aagcggttga ccgcgtccat ctccacgtcg tcgagcaact cgatgcgcgc
     4201 caccgggatc ccgagctgga tggtctggat cacggtgtcg accgcacctt tcaggccgtc
     4261 gaacgagcac accgcggccg ccatcgcttc cgggatgccg tacagccgca ccgtgacctc
     4321 ggtgatgatg ccgagcgtgc cctcgctgcc gacgaacagc cgcgtcaggt cgtaaccggc
     4381 ggccgatttg cgcgcgcggc gcgaggtgcg gatgatgcgc ccgtctgcca gcaccacggt
     4441 gagccccagc acgttctcgc gcatcgtgcc gtagcgcacg gcgttggtgc ccgaagcgcg
     4501 cgtggcggcc atgccgccga tcgaggcgtc ggcccccgga tcgatgggga aaaagagccc
     4561 cgtgtcgcgg atatgctcgt tgagctgctt gcgcgtgacg cgcgcctgca cggtcgcatc
     4621 cagatcctcg acgttgaccc gcagcacctg gtccatgttg accagatcca ccacgacgcc
     4681 gccggacttg gggatcaccg cgccctcgag cgaggtgccg gtgccgtacg ccaccaccgg
     4741 ggtgcgatgg cgcgcgcaga tctgcacgat ctgcgacacc tcctcggtgc tggcgggaaa
     4801 cgccaccgcg tcgggccggt gcgggatgtg ccacgactcg tccttgctgt ggtggtcgcg
     4861 cacgccggcg ctggtggaca ggcgctcgcc gagcagcgcg cgcagctcgt cgatcgcggt
     4921 ttggacatcg gctgcggcca ggctcatctg tgctcccacg gttgcttcgg ttgccgctcg
     4981 gcatccgaac acacgcattc tcgcagaatc gccatctgaa agacttcttt gacattgttt
     5041 gcaatcgaag gactgtatat gcaataagtg tcgccgcgga gagacagcct ggccaccggc
     5101 aagagcggcg ggccgccgcg gcgcggttcc ttcgcgcaac ttattgatcg acaagggagg
     5161 cctgcgtgcg acagcgcgcg gtatgctggt gtttggcggc agggctgggg acgctgctcc
     5221 cggccctgcc ggctgtcgcc gcggcgccgg ccctgcaggt ctttgcccag acctgggcgc
     5281 cgccgcgcgc gaccgccgga cacgacgcgc gaagcgggca ggcttgcgca ttgccccctc
     5341 agccgccatt ctctgcccgg tgcgcaaagg ttgagacggc gatgcgggtg gtgaatgtgg
     5401 gcctggtggg ctacggcttc gccgggaagg tgttccacgc gccgttgatc ggcgccgtgg
     5461 agggactgcg gctgacgcac gtggcgtcca gcgacgcagc caaagtgcag cgcgatctgc
     5521 cgcaggcgca ggtggtggcg aacgtgcagg cgctgctggc ggcgccggag atcgacctgg
     5581 tggtgatcgc cactccgaac ctgtcgcatt tcgcgctggc gcagcaggcg ctggtcgcgg
     5641 gcaagcacgt ggtggtggac aagcccttcg tggtccacac ccccgagggc gaggagctgg
     5701 cgcggctcgc cgccgcgcgc ggcaaagtgc tctccgtgtt ccacaaccgg cgctgggaca
     5761 acgacttcct caccctgcgc gagatcctgc gcagcggcgt gctgggcgcg gtgcacagct
     5821 acgaggcgca tttcgaccgc ttccgcccgc aggtgcgcga ggagcgctgg cgcgagcagg
     5881 atcagcccgg cgcgggtgtg ctctacgacc tcggctcgca cctcatcgac caggcgctgt
     5941 gcctgttcgg ccgcccgcag acggtgtggg cgcagaccgc cgtgcagcgc cccggcggca
     6001 agtcggtgga ctgcttccgc ctggtgctgg ggtatcgcga tcgcaccgcg gtgctgcgcg
     6061 cggcgagcct ggtgcgcaac ccgggcccgc gctaccaggt gcacggcacg cgcggcagct
     6121 tcatcaagca cggcctcgac ccgcaggagg acgcgctgcg tcagggcgcg cgccccggcg
     6181 cgccgggttg gggacaggaa gccgcggcgc tgttcggcga gctcgcgttc gaccacggaa
     6241 cgctgcgcat caacggcaga gtcgagacgc tgccgggctg ctaccaggcg tattacggcg
     6301 ggctgcacgc ggcgatcgcc gagggcaagg cgccaccggt tcccgcgcgc gaggcgatcg
     6361 acgtgatccg cgtgatcgaa gcggcgatga agagcgccaa cctcggggcg accatcgacc
     6421 tggcttgagt ggcgacggat gttttgacat tcatggaccc ttggcggagc acggcgatag
     6481 agtgggaggg cgcgccacgc ggcgcgccag accaagaacg cacggagacc atggaatcct
     6541 tcgagcgggc gccgatccgc gggctcgctg gcagcaccgc cgccttcctc ggcgtcgcgc
     6601 gcgccgggcc gttcgccgcc gcaccggtgg aaagcctgcg cc
//
LOCUS       DASPGE010000262         4941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1240131, whole genome shotgun sequence.
ACCESSION   DASPGE010000262 DASPGE010000000
VERSION     DASPGE010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240131"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VD791_08495"
     CDS             <1..212
                     /locus_tag="VD791_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HXV08054.1"
                     /translation="RVYAALLDGAEALTYAVPEGHRAYVHVARGSVQVNGQQLDAGDG
                     AKIAAEAQLHFGNGRQAELLLFDMA"
     gene            228..1103
                     /locus_tag="VD791_08500"
     CDS             228..1103
                     /locus_tag="VD791_08500"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HXV08055.1"
                     /translation="MLQTSPRLRDVTLVAPAHKAQPAPGLIVDRPFPGPALDRLDPFL
                     MLDHFGPEAIRPGDAGGLNPHPHRGFETVTIMLEGAMEHHDSQGNRGVIRPGGVQWMT
                     AARGIVHAEYRGQELVRQGGMLHGVQLWVNLPRRYKMNPAGYQDLGPDRIAEVPVTGG
                     VVRAIAGEYRGVAGPASTFTPLAVLHVRLESGGKADIPVADGWNAGAYLIRGAVQANG
                     RPVQERAMAVFADAGGDIEIGASAPSDVLVLAGQPIGEPVVSWGPFVMNTREEIMTAQ
                     RDYALGRMGSLPDRA"
     gene            1130..2074
                     /locus_tag="VD791_08505"
     CDS             1130..2074
                     /locus_tag="VD791_08505"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXV08056.1"
                     /translation="MSGYLRNGQWQRGWYDTRKTRGEFVRTAAAFRNRVTADGSSGFK
                     AQPGRYHLYVSLACPWAHRTVIFRALKRLEEVVTLSVVDPVMHDEGWEFSDAPGCIPD
                     AVNGFRTLREAYTAADPNYSGRVTVPVLWDRERRTIVNNESSEIIRMLNAAFDAFTPV
                     RDDYYPQALCARIDEINALVYERVNNGVYRCGFAGSQEAYDAAFDRLFSALDELETRL
                     GTSRYLVGETLTEADWRLFTTLVRFDAVYYVHFKCNLRRIAEYPNLSRLLRELYRVPG
                     VAQTVNMDHIKRHYYLSHPHLNPGGIVPKGPALDFMTA"
     gene            2137..2814
                     /locus_tag="VD791_08510"
     CDS             2137..2814
                     /locus_tag="VD791_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV08057.1"
                     /translation="MLSIRGLSKSYEGPRRRVVLRDVSLSLAPGDYVAVMGESGVGKS
                     TLLNLIAGLDRPDSGEIEVDGVPLAGLPDSALTRLRREKMGFVFQAFHVLPYLSIAQN
                     VALPLRLLGHRAGASAIKAAAMLQAVGLGDRGDSMPRELSGGELQRVAIARAVVHTPR
                     LVLADEPTGNLDAETADQVLALLRDRIKSGNAGGILVTHSRAAARTADRVYELTAEGL
                     RERQTRD"
     gene            complement(2832..4115)
                     /locus_tag="VD791_08515"
     CDS             complement(2832..4115)
                     /locus_tag="VD791_08515"
                     /inference="COORDINATES: protein motif:HMM:NF027805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HXV08058.1"
                     /translation="MSASQHTAVVLNPGHFHAALTLRQRHARLSDDVYVYAEEGSDVE
                     SFLRIVRTFNDRAQQPTGWRLHVYRGPDYLERMLAERPGEVAIVAGRNDVKMRCIHRL
                     HADGLLVLGDKPWLIHPGELTLLKETATTPPLAMDIMTERHEITSRLQKALMANPEVF
                     GRLRTDGSEPAIHIRSVHHLYKIVNQRPLVRPAWYFDTAVQGEGIMDVTTHLVDLVQW
                     MAGGGEPFRYPRDVELISARQWPTDVPRDMFARITGLEDFPAALRSRVSGGVLRYLCN
                     AEIAYRLRGVPVLIESRWDLAIPEGGGDTHFTVVRGTRADLVVEQGPETGFQTSLVVR
                     PVGTAKGFEDALAGAARSLQPSFPGLAFERAGEQFRASIPAALRTTHEEHFAAVLEEF
                     LACIDGTAALADLGADLVSKYTLLAHSAELSHRPA"
     gene            4205..>4941
                     /locus_tag="VD791_08520"
     CDS             4205..>4941
                     /locus_tag="VD791_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HXV08059.1"
                     /translation="MDVRSIAGALGAEILGVDLSRPLSAPTVADIRRAFLEHLVVVFR
                     DQRLDSQQFLAFARAMGEPIEYPFVKGLPGYPLIIPVLKLEHERSNFGGIWHSDTAYL
                     ETPPMGSMLLAREVPPVGGDTLFANQYLAYETLSPGMQRMLEGLVAVNSSAKADASRT
                     REDGMRANARDDAKREYVAEHPVARTHPETGRKSLYVNVAHTLRLRDMTEEESAPLLG
                     YLFQHQVRPEFTCRLRWEVGSLAFWDNR"
BASE COUNT          758 a         1656 c         1774 g          753 t
ORIGIN      
        1 ctcgggtcta cgccgccctg ctggacggcg ccgaggcgct gacctatgcc gtgcctgagg
       61 ggcaccgtgc gtacgtgcac gtcgcccgcg gcagcgtgca ggtgaatgga cagcagctcg
      121 acgcgggcga cggggccaag atcgcggcgg aagcgcagct gcacttcggc aacggccgtc
      181 aggccgagct gctgttgttc gacatggctt gaatcggagg agcgacgatg ctgcagacct
      241 caccccgttt gcgcgatgtg acgctggtgg cgcccgcgca caaggcccag cccgcgcccg
      301 gcctgatcgt ggaccggccg ttcccagggc cggcgctcga tcgactcgac ccgttcctga
      361 tgctcgatca cttcggcccg gaggcgatcc ggccgggcga cgccggcggc ctgaacccgc
      421 acccgcatcg aggcttcgag accgtaacca tcatgctcga gggcgcgatg gagcatcacg
      481 attcgcaggg caatcgcggc gtgatccggc ccggcggcgt gcagtggatg acggctgcgc
      541 gcggcatcgt gcacgcggag taccgcgggc aggagctggt acggcaaggc ggaatgctgc
      601 acggcgtgca gctctgggtg aatctgccgc gccgctacaa gatgaacccg gcgggctatc
      661 aggacctcgg gcccgaccgc atcgcggaag tgccggtgac cggcggtgtg gtgcgggcga
      721 tcgcgggcga ataccggggc gtcgccgggc cggccagcac gttcaccccg ctcgccgtgc
      781 ttcacgtgcg cctggagtcc ggcggcaagg cggatatccc ggtcgccgac gggtggaacg
      841 caggcgccta tctgatccgg ggcgcggtcc aggcgaacgg ccggcccgtg caggagcggg
      901 cgatggcggt gttcgcggat gcgggtggcg acatcgagat tggggccagc gcaccttccg
      961 acgtgctggt cctcgccggc cagccgatcg gcgagccggt ggtgagctgg ggaccgttcg
     1021 tgatgaacac gcgcgaggag atcatgacgg cgcagcgcga ttacgcgctg gggcggatgg
     1081 gcagtctccc cgaccgcgcc tgactctgcg tgttacggca ggcgcgcgca tgagcggcta
     1141 tctgcgcaac ggtcagtggc agcgcggctg gtacgacacg cgcaagaccc gcggcgagtt
     1201 cgtgcgcacc gcggccgcat tccgcaaccg ggtcacggcc gacggcagca gcgggttcaa
     1261 ggcgcagccg gggaggtatc acctgtacgt ctcgctggcg tgcccgtggg cgcatcgcac
     1321 ggtcatcttc cgcgcgctca agcgcctcga ggaggtcgtg acgctgtccg tcgtcgatcc
     1381 ggtcatgcac gacgagggct gggagttcag cgacgcgccc ggctgcatcc ccgacgcggt
     1441 gaacggcttc cgcaccctgc gcgaggcgta cacggcggcg gacccgaatt acagcgggcg
     1501 cgtcacggtg ccggtgttgt gggatcggga gcggcggacc atcgtcaaca acgagtcgtc
     1561 cgagatcatc cgcatgctca acgccgcctt cgacgccttc acgccggtgc gcgacgacta
     1621 ctatccgcag gcgctgtgcg cacgcatcga cgagatcaac gcgctggtgt acgagcgtgt
     1681 gaacaacggc gtgtatcgct gcggcttcgc cggcagccag gaggcctacg acgcggcgtt
     1741 cgaccggctg ttctcggcgc tcgacgagct ggagacgcgg ttgggcacct cgcgctacct
     1801 ggtgggcgag accctgaccg aagccgactg gcgcctgttc accacgctgg tgcggttcga
     1861 tgccgtctat tacgtgcact tcaagtgcaa cctgcgccgc atcgccgaat acccgaacct
     1921 gtcgcgcctg ctgcgcgagt tgtaccgggt gccgggcgtg gcgcagaccg tgaacatgga
     1981 ccacatcaag cgccactact acctgagcca tccgcacctc aacccgggcg gcatagtgcc
     2041 gaagggacca gcgctcgatt tcatgaccgc gtagcccgga tgcagcgaag cggaatccgg
     2101 ggatgatggg gctcgtctct tcggtatgat gcgccggtgc tgtcgatccg cggcctgagc
     2161 aagtcctacg agggtccgcg ccgccgtgtg gtgctgcgcg acgtgagctt gtcgctggcg
     2221 ccgggcgact acgtcgccgt gatgggcgag tcgggcgtag gcaagtccac gctgctcaac
     2281 ctgatcgccg gcctcgaccg ccccgacagc ggcgagatcg aagtcgacgg ggtgccgctc
     2341 gcggggttgc cggacagcgc gctcacacgg ctgcgccgcg agaagatggg cttcgtgttc
     2401 caggcgttcc atgtgctgcc ttatctgagc attgcgcaga acgtggccct gccgctgcgc
     2461 ctgctcggcc atcgcgccgg cgcgagcgcg atcaaggcgg ccgcgatgct gcaggccgtc
     2521 ggcctggggg accgcggcga cagcatgccg cgcgagctct ccggcggcga gctgcagcgc
     2581 gtggcgatcg cgcgcgccgt ggtgcatacg ccgcgcctgg tgctcgccga cgagcccacc
     2641 ggcaatctcg acgccgagac ggcggaccag gtgctggcgc tgctgcgcga tcggatcaag
     2701 agcggcaacg ccggcggcat cctggtgacc cactcgcgcg ccgctgcgcg aaccgcggac
     2761 cgcgtgtacg agctcaccgc ggagggcctg cgcgaaaggc agacccgcga ctaggcgcag
     2821 aggcccggct gtcaggcagg gcggtgggac agctccgcag agtgcgcgag cagcgtgtac
     2881 ttggacacca gatcggcgcc gagatcggcg agggccgctg tgccgtcgat gcaggcgaga
     2941 aactcctcca gcacggcggc gaagtgctcc tcgtgggtgg tgcgcagcgc agccgggatc
     3001 gacgcgcgaa actgctctcc ggcgcgctcg aaggcaagcc ccggaaagct cggttgaagg
     3061 gagcgcgccg caccggccag ggcgtcttcg aagcccttcg cggtgccgac ggggcgcacg
     3121 accagcgagg tttggaagcc cgtctccggt ccctgctcga cgaccaggtc ggcccgcgtg
     3181 ccgcgcacca ccgtgaagtg cgtgtcgccg ccgccttccg ggatcgccag atcccagcgc
     3241 gactcgataa gcaccggcac gccgcgcagc cgataggcga tctccgcgtt gcacagatag
     3301 cgcagcacgc cgccggagac gcggctgcgc agcgccgccg ggaaatcctc gagcccggtg
     3361 atgcgcgcga acatatcgcg cgggacgtcg gtcggccatt gccgcgcgga gatgagctcc
     3421 acgtcgcgcg ggtagcgaaa aggctctccg ccgcccgcca tccattggac gagatccacc
     3481 aggtgcgtgg tgacgtccat gatgccctcg ccttgcactg ccgtatcgaa gtaccaggcc
     3541 gggcgcacca gaggacgctg gttcacgatc ttgtacaagt ggtgcacgct gcggatgtgg
     3601 atcgccggct cgctgccgtc ggtgcgcaat cgcccgaaca cttccgggtt ggccatgagc
     3661 gccttctgca gccggctggt gatctcgtgg cgctcggtca tgatgtccat ggcgagcggc
     3721 ggcgtggtgg cggtctcctt cagcagcgtc agctcgccgg gatggatcag ccacggcttg
     3781 tcgcccagga ccaggaggcc gtcggcgtgc aggcggtgga tgcagcgcat cttgacgtcg
     3841 ttgcggccgg cgacgatcgc cacctcgccg gggcgctccg ccagcatccg ctccaggtag
     3901 tcggggccgc gatagacgtg cagccgccaa cccgttggct gctgcgcgcg gtcgttgaac
     3961 gtgcgcacga tgcgcaggaa gctctcgacg tcggacccct cctcggcgta gacgtacacg
     4021 tcgtcgctga ggcgcgcgtg ccgctggcgc agcgtgagcg cggcgtggaa gtgaccgggg
     4081 ttgagcacca cggcggtgtg ctgactggcg gacatactgg cctcgtccgg ggcctcgtct
     4141 gggttgtctg ctcgggattg tacagccgct atactggcag gcgtcgccag gcggcaggag
     4201 gggcatggac gtcaggagca ttgcgggcgc gctgggagcc gaaattctgg gcgtggatct
     4261 gtcgcgcccg ctgtccgcgc ccaccgtcgc cgacatccgc cgcgcgttcc tcgagcacct
     4321 ggtcgtggtc ttccgcgatc agcggctcga ttcgcagcag ttcctggcgt tcgcgcgcgc
     4381 catgggcgag ccgatcgagt accccttcgt caaggggctg cccggttacc cgctgatcat
     4441 cccggtgctc aagctcgagc acgagcgcag caacttcggc ggcatctggc attccgacac
     4501 cgcctacctg gagacgccgc cgatgggctc gatgctgctc gcccgcgaag tgccgccggt
     4561 gggcggcgac acgttgttcg cgaatcagta cctcgcttac gagacgctgt cccccggcat
     4621 gcagcgcatg ctggaagggc tggtcgcggt gaacagctcg gccaaggccg acgcctccag
     4681 gacccgcgag gacggcatgc gggccaacgc gcgcgacgac gcaaagcgcg agtacgtcgc
     4741 cgagcatccg gtggcgcgca cgcatcccga gaccggacgc aaatcgctct acgtgaacgt
     4801 cgcgcacacg ctgcgcctcc gggacatgac cgaggaggaa agcgcgccgc tgctcggcta
     4861 cctgttccag caccaggtgc gtcccgagtt cacctgccgc ttgcgctggg aagtgggctc
     4921 gctcgcgttc tgggacaacc g
//
LOCUS       DASPGE010000263         9024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_305489, whole genome shotgun sequence.
ACCESSION   DASPGE010000263 DASPGE010000000
VERSION     DASPGE010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9024
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305489"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VD791_08525"
     CDS             <1..809
                     /locus_tag="VD791_08525"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HXV08060.1"
                     /translation="DRLKGTRIVMDLVTVTGTENLMMAATLAEGTTVIENAAREPEVA
                     DLADCLVAMGAQISGQGSDVVTIVGVPQLRGATYSVMPDRIEAGTFLVAAAATGGEIA
                     LRGMRPGILDAVLTKLVEAGARVDNGPDRLALRAHGGLKATSVRTAPYPAFPTDMQAQ
                     FMALDAVAEGTAVITETVFENRFMHVHELRRLGANIEVEGNTAIVRGVEALSGASVMA
                     TDLRASACLVVAGLMAEGETLVDRIYHLDRGYERIEEKLSQLGARIRRVR"
     gene            849..1514
                     /gene="hisG"
                     /locus_tag="VD791_08530"
     CDS             849..1514
                     /gene="hisG"
                     /locus_tag="VD791_08530"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HXV08061.1"
                     /translation="MNRITIALSKGRIFSESLPLLAAAGVQPAEDPETSRRLIIGTGR
                     ADVSIIVVRASDVPTYVRYGAADLGIAGKDVLHEQGSEGLYQPLDLRIARCRMMVAVP
                     ADFDYATAVRQGARLRVATKYLKTAREHFAAKGVHVDLIKLYGSMELAPLVGLADAIV
                     DLVSTGSTLRANNLKAVEEIMPISARLIVNQAALKLKGALLEPLLQAFARAVAPEDAD
                     AAA"
     gene            1501..2802
                     /gene="hisD"
                     /locus_tag="VD791_08535"
     CDS             1501..2802
                     /gene="hisD"
                     /locus_tag="VD791_08535"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HXV08062.1"
                     /translation="MLQLNILSTRDADFEARLAGLLARGSEQDESIERAVADILSDVR
                     RRGDEAVLEYTRRFDRVQAAGVAQLEVPRSAVERALEQLPPPRREALVQAAARIRTYH
                     ERQLVQSWSYTDEDGTRLGQQVTPLDRVGIYVPGGKAAYPSSVLMNALPARVAGVPEI
                     VMTTPTPDGERNELVLAAAAVCGVNRVFAIGGAQAIAALAYGTASVPRVDKIVGPGNA
                     YVAEAKRRVFGRVGIDMIAGPSEILVLCDGKTSPDWVAMDLFSQAEHDEMAQAILLSP
                     DEGFVRAVGDSIRRQLADMPRREVIAASLTARGALIAVRDLEEACAIANRIAPEHLEL
                     SVEEPERWLPRLRHAGAIFLGRYSSEALGDYCAGPNHVLPTAGTARFSSPLGVYDFQK
                     RTSLIQVSQAGARKLGEIAVELALGEGLPAHAQAARYRWHG"
     gene            2808..3881
                     /gene="hisC"
                     /locus_tag="VD791_08540"
     CDS             2808..3881
                     /gene="hisC"
                     /locus_tag="VD791_08540"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HXV08063.1"
                     /translation="MTPEQVIRDEVRALAAYHVPESQGMVKLDAMENPYGLPREIAEE
                     IARLAAAAPLNRYPDPAARTLKLRLRETMSIPEGAELLLGNGSDELIQMLALAAARPG
                     AVLLGVEPSFVMFRLIAGFAGMRYVGVPLGPGFGLDLPALESAVREHRPALIFLAYPN
                     NPTGNLFDAQAMERIIAMSPGLVVVDEAYHVFAGASFLPRLAACANLLVMRTLSKLGL
                     AGLRLGLLAGAPQWLREVDKVRLPYNVNVLSQQVAELVLRHPEVLDRQAAAIRAERTR
                     LLARLTGLTGVQAFPSEANFILFRTAKADAVFAALKARAVLIKNLNGSHPMLADCLRV
                     TVGTPEENDRFVDALSASLQRAA"
     gene            3967..4554
                     /gene="hisB"
                     /locus_tag="VD791_08545"
     CDS             3967..4554
                     /gene="hisB"
                     /locus_tag="VD791_08545"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HXV08064.1"
                     /translation="MRQAEVVRKTLETQITVKLNLDGSGKSALATGIGFFDHMLDQVA
                     RHGMLDLDIAATGDLHIDAHHTVEDVGIALGQAVAKALGDKTGVRRYGHAYVPLDEAL
                     SRVVIDLSGRPGLEFDIRFVRGLIGEFDVDLIHEFFQGFVNHAQVTLHVDNLKGKNAH
                     HQAETAFKAFGRALRMAVELDSRMQGILPSTKGKL"
     gene            4567..5259
                     /gene="hisH"
                     /locus_tag="VD791_08550"
     CDS             4567..5259
                     /gene="hisH"
                     /locus_tag="VD791_08550"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HXV08065.1"
                     /translation="MGGQVDIAVIDYGMGNLRSVAKAIEHVAAGLRVAVTDDPDAVRA
                     ARRVVFPGQGAMPDCMRELDARGLRPAVVEAAAGKPFLGICIGLQMLFEESEEGGVRG
                     LNLLRGRVVRFPGERMQDATGARLKVPHMGWNEVHQTLRHPLWEGIASGSRFYFVHSY
                     YPAPQDRSIVAGYTLYPFPFACAVARANLFAVQFHPEKSQAAGLRLLRNFVGWDGRYA
                     RPEAHAAVPELR"
     gene            5333..6091
                     /gene="hisA"
                     /locus_tag="VD791_08555"
     CDS             5333..6091
                     /gene="hisA"
                     /locus_tag="VD791_08555"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HXV08066.1"
                     /translation="MLIIPAIDLKDGHCVRLKQGAMDKATVFSDDPAAMARHWLAQGA
                     RRLHLVDLNGAFAGRPRNEEAIKAVVAAVGESVPVQLGGGIRDLDTVERYIDDGITYV
                     IIGTAAVKTPGFLHDACYAFPGHVMVALDAKDGKVAVDGWSKMTGHDVIDLAKKFQDY
                     GVEAVIYTDIGRDGMLTGVNIEATVELARALSVPVIASGGITGLDDVKALCQVEQEGI
                     VGAITGRALYQGSLSFAEAQKLADQLADQLAAKQ"
     gene            6159..6989
                     /gene="hisF"
                     /locus_tag="VD791_08560"
     CDS             6159..6989
                     /gene="hisF"
                     /locus_tag="VD791_08560"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HXV08067.1"
                     /translation="MLAKRIIPCLDVTSGRVVKGVNFVGLRDAGDPVEIARRYDEQGA
                     DELCFLDITASSDERGILLHVIESVAEQVFIPLTVGGGVRSVTDVRALLNAGADKVSI
                     NTAAVLDPEVVAQASGKVGSQCIVVAIDAKRVSENTGRATENTGRVSRDGDAAAGGRW
                     QVFTHGGRRPTGLDAVQWARRVQALGAGEILLTSMDRDGTRDGYDLELTRAVSDSVDI
                     PVIASGGVGNLDHLVEGILQGHADAVLAASIFHYGEYTVRQAKQHLAARRIEVRDLSG
                     "
     gene            7014..7463
                     /gene="hisI"
                     /locus_tag="VD791_08565"
     CDS             7014..7463
                     /gene="hisI"
                     /locus_tag="VD791_08565"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HXV08068.1"
                     /translation="MTADWLDQVKWDADGLVATIAQDAASGRVLMLAWMNREALRLTL
                     ETGAAVYWSRSRRRLWRKGEESGHVQTVREIRLDCDADALLLQVEQAGGIACHTGRER
                     CFFRRQEGGGWVETDPVLKSPEDIYRLEDSKQPGDIKRPRDVKRKAP"
     gene            7460..7786
                     /locus_tag="VD791_08570"
     CDS             7460..7786
                     /locus_tag="VD791_08570"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HXV08069.1"
                     /translation="MIDPSILHRLGEALEARKQADPSSSYTAQLFAKGHDAILKKVAE
                     EAAETLMAAKDGDRLHIVREIADLWFHTLVLLSHHGLGPDDVLAELRRREGISGIDEK
                     AGRPRD"
     gene            7877..8239
                     /locus_tag="VD791_08575"
     CDS             7877..8239
                     /locus_tag="VD791_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HXV08070.1"
                     /translation="MHDPNCVFCKIVRGEIPSRKAYEDDLVLAFHDIHPIAPVHLLIV
                     PKAHVATLADAQAGHEPALGRMLGVAARLATEQGSPDGFRMIVNTGRVGRQEVMHVHA
                     HILGGNEPLGGMLPRSRP"
     gene            8265..8495
                     /gene="tatA"
                     /locus_tag="VD791_08580"
     CDS             8265..8495
                     /gene="tatA"
                     /locus_tag="VD791_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HXV08071.1"
                     /translation="MGSFSIWHWLIVLVVVVLIFGTKKLRNLGTDLGNAVKGFKEGIK
                     TEEDKAPTAGAAKIEHAGGQTIEGEVKDRAKS"
     gene            8536..>9024
                     /gene="tatB"
                     /locus_tag="VD791_08585"
     CDS             8536..>9024
                     /gene="tatB"
                     /locus_tag="VD791_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HXV08072.1"
                     /translation="MLDIGFTELMLIGVVALIVIGPERLPRVARTAGHLLGRFQRYVS
                     EVKADINREIELADLKKIQSSVEDAARSIERSVKSEMHEAEETLRSAEAELTEAGAAL
                     EKTGTELNQALTMPHMGMLGSTATSAPDPDIAADKAATDSAQPLPPGLASATGATQPD
                     HTP"
BASE COUNT         1437 a         3082 c         3202 g         1303 t
ORIGIN      
        1 ccgaccggct gaaagggacg cgcatcgtca tggacctggt gaccgtgacg ggcaccgaaa
       61 acctgatgat ggccgcgacc ctggccgagg ggaccacggt catcgagaat gcggcgcgcg
      121 agcccgaggt ggcggatctg gccgactgcc tggtggccat gggtgcccag atctccggtc
      181 agggcagcga cgtcgtcacc atcgtcgggg tgccgcagct gcgcggggcg acctattcgg
      241 tgatgcccga ccggatcgag gccggcacct tcctggtggc ggccgccgcc accggcggtg
      301 agatcgcgct gcgcggcatg cgccccggca tcctcgacgc ggtgctgacc aagctggtcg
      361 aggccggagc acgcgtggac aacggtcccg accggctcgc gctgcgcgcg cacggcgggc
      421 tcaaggccac cagcgtgcgt accgcgccct atcccgcgtt ccccaccgac atgcaggcgc
      481 agttcatggc gctcgacgcc gtggccgagg gcaccgcggt gatcaccgag accgtgttcg
      541 aaaaccgttt catgcacgtg cacgagttgc gacggctggg cgccaatatc gaggtggaag
      601 gcaacaccgc catcgtgcgc ggcgtggagg cgctgtccgg tgccagcgtc atggccaccg
      661 acctgcgcgc ttcggcctgc ctggtggtcg ccggcttgat ggccgagggc gagacgctgg
      721 tcgatcgcat ctaccatctc gatcgcggct acgagcgcat cgaggagaag ctgtcgcagc
      781 tcggcgcgcg gattcggcgg gtgcgctaat cgcgtagaat cccgactcca gtattcgggg
      841 ccgaccccgt gaaccgcatc accatcgcgc tctccaaggg ccgcatcttc agcgagagcc
      901 taccgctgct cgccgccgcg ggcgtccagc cggcggaaga cccggaaact tcgcgccgcc
      961 tgatcatcgg caccggccgc gcggatgtca gcatcatcgt ggtgcgcgcc tccgacgtcc
     1021 cgacctacgt gcggtacggc gccgctgacc tgggcatcgc cggcaaggac gtcctgcacg
     1081 agcagggctc ggagggtctg taccagccgc tggacctgag gatcgcgcgc tgccggatga
     1141 tggtggcggt gcccgccgac ttcgattacg ccaccgcggt gcgccagggc gcgcgcctgc
     1201 gggtcgcgac caagtacctg aagacggccc gcgaacattt cgccgccaag ggcgtgcacg
     1261 tagacctgat caagctgtac gggtcgatgg agctggcgcc gctggtggga ttggccgacg
     1321 ccatcgtcga cctggtcagc accggcagca cgctgcgcgc caacaacctg aaggccgtgg
     1381 aagagatcat gccgatcagc gcgcggctga tcgtcaacca ggccgcgctc aaactgaagg
     1441 gcgcgctgct cgagccgctg ctgcaggcgt tcgcacgcgc ggtggcaccc gaagacgccg
     1501 atgctgcagc ttaacatcct gtcgacgcgg gacgccgact tcgaagcgcg cctggcgggt
     1561 ctgctcgccc gcgggagcga gcaggacgag tcgatcgagc gcgcagtcgc cgatatcctg
     1621 agcgacgtgc gccgtcgcgg cgacgaggcg gtgctcgagt acacgcgtcg cttcgaccgg
     1681 gtgcaggcgg ccggcgtggc gcagctggag gtcccgcgca gcgctgtgga gcgcgccctc
     1741 gagcagcttc cgccgccgcg gcgcgaggcg ctggtgcagg ccgcggcccg catccgcacc
     1801 taccacgaac gccagctggt gcagtcctgg agctacaccg acgaggacgg cacgcgcctg
     1861 gggcagcagg tgacgccgct cgatcgcgtc ggcatctacg tgccgggcgg taaggccgcc
     1921 tatccgtcct cggtgctgat gaacgcgctg cccgcgcgcg tggcgggcgt gcccgagatc
     1981 gtcatgacca cgccgacgcc ggacggcgag cgcaacgagc tggtgctggc cgctgccgcg
     2041 gtgtgcggcg tgaaccgtgt gttcgcgatc ggcggggcgc aggcgatcgc cgcgctcgcg
     2101 tacggcaccg catcggtgcc ccgggtcgac aagatcgtcg ggccgggcaa cgcctacgtc
     2161 gcggaagcca agcggcgggt gttcggccgg gtcggcatcg acatgatcgc cgggccgtcc
     2221 gagatcctgg tgctgtgtga cggcaagacc agccccgact gggtggccat ggatctgttc
     2281 tcccaggccg agcacgacga gatggcccag gcgatcctgc tcagccccga cgaggggttc
     2341 gtgcgtgccg tgggcgacag catccggcgc cagctcgcgg acatgccgcg gcgcgaggtg
     2401 atcgcggctt cgctcaccgc gcgcggggcg ctgatcgccg tgcgcgatct cgaggaagcc
     2461 tgcgccatcg ccaaccgcat cgcgcccgag cacctcgagc tgtcggtgga ggaacccgag
     2521 cgctggctgc cgcggctgcg ccacgccggc gcgatcttcc tgggccgcta cagctcggag
     2581 gcgctgggcg actactgcgc cggccccaac cacgtgctgc ccaccgccgg caccgcgcgc
     2641 ttctcctcgc cgctgggcgt ctacgacttc cagaagcgca ccagcctgat ccaggtgtcc
     2701 caggccggcg cgcgcaagct gggcgagatc gccgtcgagc tggccctcgg cgagggcctg
     2761 ccggcgcacg cgcaggccgc gcgctaccga tggcacgggt gatggcgatg acacccgaac
     2821 aggtgatccg cgacgaggtc cgcgcgctgg cggcctatca cgtgcccgag agccagggca
     2881 tggtcaagct cgacgcgatg gagaacccct acggcctgcc gcgcgagatc gcggaggaga
     2941 ttgcgcggct cgccgccgcc gcgccgctca accgctatcc cgatccggcc gcgcgcaccc
     3001 tcaagctgcg cctgcgcgag acgatgtcta taccggaggg cgcggagctg ctgctcggca
     3061 acggctcgga cgagctgatc cagatgctgg cgctggcggc ggccaggccg ggcgcggtgc
     3121 tgttgggcgt ggagccgtcg ttcgtgatgt tccgcctgat cgccggcttc gccggcatgc
     3181 gctacgtcgg cgtgccgctc ggcccgggct tcggcctgga cctgccggcg ctggaatcgg
     3241 cggtgcggga gcaccggccc gccctgatat ttttggcgta cccgaacaat ccgaccggca
     3301 atctgttcga tgcgcaggcg atggagcgca tcatcgcgat gagtcctggc ctggtggtgg
     3361 tggacgaggc gtatcacgtc ttcgccggcg cgagcttcct gccgcgcctg gccgcgtgcg
     3421 ccaacctgct ggtgatgcgc acgctgtcca agctcggcct ggccggcctg aggctgggcc
     3481 tgctggccgg cgccccgcag tggttgcgcg aggtcgacaa ggtacggctg ccctataatg
     3541 tgaacgtcct gtcgcagcag gtggccgagc tggtgttgcg gcaccccgag gtgctcgacc
     3601 ggcaggcggc cgcgatccgg gccgagcgca cgcgcctgct ggcgcgcctg accgggctca
     3661 caggcgtgca ggcgtttccc agtgaggcga acttcatcct gttccgcacc gccaaggccg
     3721 atgcggtgtt cgcggcgctc aaggcgcgcg cggtgctgat caagaacctg aacggctcgc
     3781 acccgatgct cgccgattgc ctgcgcgtga ccgtcggcac acccgaggaa aacgaccgct
     3841 tcgtcgacgc cctgagcgca agcctgcagc gcgcagcctg aactcgagcg ccgccgccag
     3901 cgccgccctg ctcaccgatc cgccgcatcg cccgcttcaa tcgcccagcc acttcagcta
     3961 ctccccatgc gccaggccga agtcgtccgc aagacgctcg agacgcagat cacggtcaag
     4021 ctgaacctcg acggcagcgg caagtccgcg ctcgccaccg gcatcggctt cttcgatcac
     4081 atgctcgacc aggtggcgcg gcacgggatg ctggacctcg acatcgccgc gaccggagat
     4141 ctgcacatcg acgcgcatca caccgtggaa gacgtcggca tcgcgctcgg ccaggccgtg
     4201 gccaaggcgc tcggcgacaa gaccggtgtg cgccgctatg gccacgccta cgtgccgctg
     4261 gacgaggcgc tgtcgcgcgt ggtgatcgac ctgtccgggc gtcccggcct cgagttcgac
     4321 atccggttcg tgcgcgggct gatcggcgag ttcgacgtgg acttgatcca cgagttcttc
     4381 cagggtttcg tcaaccacgc gcaggtcacc ctgcacgtgg acaacctgaa gggcaagaac
     4441 gcgcaccacc aggccgagac cgcgttcaag gccttcggcc gggcgctgcg catggcggtg
     4501 gagctcgact cgcgcatgca gggcatcctg ccttcgacca agggcaaact ctagggagca
     4561 aacccggtgg gcggacaggt cgacatcgcc gtgatcgact acggcatggg caatctgcgg
     4621 tcggtggcca aggccatcga gcacgtggcc gcggggctgc gggtggcggt caccgacgac
     4681 cccgatgcgg tgcgcgcagc gcgccgcgtg gtgtttcccg gccagggcgc gatgcccgac
     4741 tgcatgcgcg agttggacgc gcgcggattg cgtccggcgg tggtcgaggc cgccgccggc
     4801 aagcccttcc tgggcatctg catcggattg caaatgctgt tcgaggagag cgaggagggc
     4861 ggcgtgcgcg gtctcaacct gctgcgcggc cgggtggtgc gcttcccggg tgagcgcatg
     4921 caggacgcga ccggtgcgcg tctcaaggtg ccgcacatgg gctggaacga agtgcaccag
     4981 acgctgcgcc atccgctgtg ggagggcatc gcgagcggca gccgcttcta cttcgtgcac
     5041 agctattacc ccgccccgca ggaccggtcg atcgtcgccg gctacaccct gtatccgttc
     5101 ccgttcgcct gcgcggtggc gcgcgccaac ctgttcgccg tgcagttcca ccccgagaag
     5161 agccaggccg cgggcctgcg gctgctgcgc aacttcgtcg gctgggacgg gcgctacgcg
     5221 cggcccgaag cgcacgccgc ggtgccggaa cttcgctagg cgctttgctg tatgatcgct
     5281 cgcccgtccc gcctccccgc tcgttgatct ccggcttccc gccgctcccc gcatgctgat
     5341 cattccggcg atcgatctga aagacggtca ttgtgtgcgc ttgaagcagg gcgccatgga
     5401 caaggccacc gtgttctccg acgatccggc ggcgatggcg cgccactggc tggcgcaggg
     5461 cgcgcggcgc ctgcatctgg tggacctgaa cggcgcgttc gccggtcggc cgcgcaacga
     5521 ggaggcgatc aaggccgtgg tcgcggccgt cggcgagtcg gtgccggtgc agctcggcgg
     5581 cggcatccgc gacctcgaca ccgtcgagcg ctacatcgac gacggcatca cttacgtgat
     5641 catcggcacc gccgcggtga agacgcccgg tttcctgcac gacgcctgct acgccttccc
     5701 cggccacgtg atggtcgcgc tcgacgccaa ggacggcaag gtggcggtgg acggctggtc
     5761 caagatgacc ggccacgacg tcatcgacct ggcgaagaag tttcaggatt acggcgtgga
     5821 ggcggtcatc tacaccgaca tcggccgcga cggcatgttg accggcgtca acatagaggc
     5881 caccgtggag ctggcgcgcg cgctcagcgt cccggtgatc gccagcggcg ggatcaccgg
     5941 actcgacgac gtcaaggcgc tgtgccaggt ggagcaggaa ggcatcgtcg gcgcgatcac
     6001 cggccgtgcc ctgtatcagg gcagcttgag cttcgccgag gcccagaagc tcgcggacca
     6061 gctcgccgac cagctcgccg ccaagcagta gcgccgcacc gctccggttc gcctgccggt
     6121 cgcgcgcagc gatacggatc ttcgatctct gattccgaat gctcgccaaa cgcatcatcc
     6181 cctgtctcga cgtcaccagc gggcgcgtgg tcaagggcgt caacttcgtc ggcctgcgcg
     6241 acgccggcga cccggtggag atcgcgcgcc gctacgacga gcagggcgcc gacgagctct
     6301 gcttcctcga catcacggcc agctcggacg agcgcggcat cctgctgcac gtcatcgaat
     6361 cggtggccga gcaggtgttc atcccgctca ccgtgggcgg gggcgtacgc tcggtgacgg
     6421 acgtgcgggc gctgctcaat gccggcgccg acaaggtgag catcaacacc gccgcggtgc
     6481 tcgatcccga ggtggtggca caggcgagcg gcaaggtggg atcgcagtgc atcgtggtgg
     6541 cgatcgacgc caaacgcgtg tctgaaaaca ccgggcgcgc gactgaaaac accgggcgcg
     6601 tgagccggga cggcgacgct gcagcgggcg ggcgctggca ggtgttcacc cacgggggac
     6661 gccgccccac cgggctggac gcggtgcagt gggcccggcg tgtgcaggcg ctgggcgccg
     6721 gcgagatcct gctcaccagc atggatcgcg acggcacccg cgacggctac gatctggagc
     6781 tcacgcgggc ggtttcggac agcgtggaca tcccggtcat cgccagcggg ggcgtcggca
     6841 acctggacca cctggttgag ggtatcctgc aagggcacgc cgacgcggtg ctggccgcca
     6901 gcatcttcca ttacggcgag tacaccgtgc gccaggcgaa gcagcacctg gccgcgcgtc
     6961 gcatcgaagt gcgggacctg tccgggtgat tcgaccgggc gaacggatgc cgaatgacag
     7021 ccgactggct ggatcaagtg aagtgggacg cagacgggct ggttgccacg atcgcgcagg
     7081 acgccgcctc gggccgcgtg ctgatgctcg cctggatgaa tcgggaggcg ctgcggctca
     7141 ccctggagac cggcgcggcg gtgtactggt cgcgctcccg ccggcgcctg tggcgcaagg
     7201 gcgaggaatc cggtcacgtg cagacggtgc gcgagatccg gctcgattgc gacgccgatg
     7261 cgctgctgct gcaggtggag caggccggcg gcatcgcctg ccacaccggc cgcgagcgct
     7321 gcttcttccg ccggcaggaa ggcggcggct gggtcgaaac cgatcccgtg ctcaagtccc
     7381 ccgaggacat ctaccggctt gaggacagca agcagcccgg ggacatcaag cggccccggg
     7441 acgtcaagcg aaaggcgcca tgatcgaccc ctccatcctg caccgcctgg gcgaggccct
     7501 ggaagcgcgc aagcaggccg acccgtcctc ctcgtacacg gcgcagctgt tcgccaaggg
     7561 tcacgacgcc attctcaaga aggtcgcgga ggaagccgcc gagaccctga tggcggccaa
     7621 agacggtgat agactgcaca tcgtccgcga gatcgcggac ctgtggtttc acactctcgt
     7681 gctgctgagc caccacgggc tgggcccgga cgatgtactg gccgagctgc ggcggcgcga
     7741 agggatttcg ggaatagacg agaaggcggg gcgaccgcgc gactgaccac cgcccgcagg
     7801 aggccgcacc gccgccaggc gggccggcgc tgcgcgacgt tgcgccggcg cgagccgtct
     7861 ggactgcccc caaacgatgc acgatccgaa ctgcgtcttc tgcaagattg tcagagggga
     7921 gataccgtcc cgcaaggcct acgaggacga tctggtgctc gccttccacg acatccaccc
     7981 gatcgcgccg gtgcacctgc tgatcgtgcc gaaagctcac gtcgcgacgc tggccgacgc
     8041 gcaggccggg cacgagccgg cgctcggcag gatgctgggc gtggccgccc gcctcgccac
     8101 cgagcaggga tcgccggacg gcttccgcat gatcgtcaac accgggcggg tggggcggca
     8161 ggaggtcatg cacgtgcacg cccacatcct gggcggcaac gaaccgctgg gcggcatgct
     8221 gccgcggtcc agaccgtaat caactctagc ttaaggagaa acgtatggga tcgttcagca
     8281 tctggcactg gctcatcgtg ctggtggtgg tggtgctgat cttcggcacc aagaagctgc
     8341 gcaacctcgg caccgacctg ggcaacgccg tgaagggctt caaggaaggc atcaagaccg
     8401 aggaagacaa ggcgccgacg gcgggtgcag ccaagatcga acacgccggc ggccagacca
     8461 tcgagggcga agtgaaggac cgggcgaagt cctgacgccc ggcgtgcggg cggctgctgc
     8521 gcgctggagc gcgagatgct cgacatcggg ttcaccgagc tgatgctgat cggggtggtg
     8581 gcgctgatcg tcatcggccc cgagcgtttg ccgcgggtcg cgcgcaccgc gggccacctg
     8641 ctggggcgct tccagcgcta cgtgtccgag gtgaaggcgg acatcaatcg cgagatcgag
     8701 ctggcggacc tgaagaagat ccagtccagc gtcgaggacg cggcgcgctc catcgagcgc
     8761 tcggtcaaga gcgagatgca cgaggcggag gaaacgctgc gcagcgccga agccgagttg
     8821 accgaggccg gcgcagcgct cgagaagacc ggaaccgagc tgaaccaggc gctcaccatg
     8881 ccgcacatgg gcatgctggg ctccacggcg acctcggcac cggacccgga catagcggcg
     8941 gacaaggcgg cgacggactc cgcgcaaccg ctgccgccgg ggctcgccag cgcaaccggc
     9001 gccacacagc ccgaccacac gccg
//
LOCUS       DASPGE010000264         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_421537, whole genome shotgun sequence.
ACCESSION   DASPGE010000264 DASPGE010000000
VERSION     DASPGE010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421537"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1652
                     /locus_tag="VD791_08590"
     CDS             117..1652
                     /locus_tag="VD791_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08073.1"
                     /translation="MTNAAGGARWWVAGDLNGFFGLFTNVLLNVIVLTTLCLYVVNLP
                     PDTVYGRVLPALGIALPLGNLFYAYLAWQLAKKEGRSDVTAMPYGPSVPHMFIVVLVI
                     MLPTYLATNDPMLAYQAGLAWCFIIGVIVLIGAFVGPWIRKVTPRAAMLGTLAGISIA
                     FISMRPAFQMWEVPWIGLACFAIVLIAWTANKRLPGGIPGGLAAVILGAAIGWIAAAF
                     GWSDYMKGEQVTKSFESFGLHLPFFSSHVFSGLASIGPLLATAIPLGIYNFTEAMNNV
                     ESASAAGDNYNLRKILLADGAGAIVGSMLGSPFPPAVYIGHPGWKAVGGRIGYSLATG
                     VAIALVCFLGLTALLLNVIPLVAILPILLYIGLVIGAQAFQASPSKHAPAVVLAIIPN
                     VAEWAKISMDSALQVAGSSADKVGFAALAQVGVVYQGMALLGGGSVLAGMVLGAMAVF
                     IIDHKLKHAAAAAFIGAVLAFVGLIHAPQLALGASPLVALGYAMFGALCLAAELTDRK
                     ATA"
     gene            complement(1662..>2425)
                     /locus_tag="VD791_08595"
     CDS             complement(1662..>2425)
                     /locus_tag="VD791_08595"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HXV08074.1"
                     /translation="DVPGVGEDLQDHFYCRTFWRCNRPITLNDDMLSRWRQAKIGLQY
                     LLFRRGPLTVSAGYAGAFVRTQPQAARPDVQVYFINFSTAKRGGTLHPFSGFTLSVSQ
                     TQVESRGSVHIASADPRAAPAIRYNYLSTENDRRVMVDGLKLVRRIAVTPPLRDYVVA
                     EEYPGPGVRSDEDLLAFCREAGDTVFHPTSTCRMGTDGRAVVDPQLRVQGVERLRVVD
                     ASVIPAVPSGNINAAVIAVAEKAADLVAETGRDLR"
BASE COUNT          368 a          810 c          840 g          407 t
ORIGIN      
        1 tcaccgaccc gcccaacctg gggcgggccc tggcaggagc gaccggcaca cgcgttcacc
       61 gctgagaaat atccgggctg gcagttcacc acaaccacaa caggagggag aggaccatga
      121 cgaacgccgc agggggtgcc cgctggtggg tggcgggcga cttgaacgga ttcttcgggc
      181 tgttcaccaa cgtgctgctc aacgtgatcg tcctgaccac gttgtgcctg tacgtggtca
      241 accttccgcc cgacaccgtg tacggacggg tgctgccggc gctggggatc gcgctgccgc
      301 tgggcaatct gttctacgcc tatctcgcct ggcagctcgc caagaaggag gggcgctcgg
      361 acgtgacggc gatgccctac gggccgagcg taccgcacat gttcatcgtg gtgctggtga
      421 tcatgctgcc cacctacctc gcaaccaacg acccgatgct cgcctaccag gccggcctgg
      481 cgtggtgctt catcatcggc gtgatcgtgc tgatcggcgc gttcgtcgga ccgtggatcc
      541 gcaaggtcac cccgcgcgcg gcgatgctgg gcaccctggc cggcatctcg atcgcgttca
      601 tctcgatgcg tccggcattc cagatgtggg aggtgccgtg gatcgggctg gcgtgcttcg
      661 ccatcgtgct gatcgcctgg accgccaaca agcgcctgcc gggcgggatt cccggggggc
      721 tggcggcggt gatcctgggc gccgcgatcg gctggatcgc cgcggccttc ggctggagcg
      781 actacatgaa gggcgagcag gtgaccaagt cgttcgagag cttcggcctg cacctgccgt
      841 tcttctcctc gcacgtcttc agcggcctgg cgagcatcgg tccgctgctc gccaccgcca
      901 ttccattggg catctacaac ttcaccgagg cgatgaacaa cgtcgagagc gcctcggcgg
      961 cgggggacaa ctacaatctg cgcaagatcc tgctcgccga cggcgccggc gcgatcgtcg
     1021 ggtcgatgct cggcagcccg ttcccgccag cggtctacat cggccatccg ggctggaagg
     1081 cggtgggagg ccggatcggc tactcgctcg ccaccggcgt ggccatcgcg ctggtctgct
     1141 tcctcggcct gaccgccttg ttgctcaacg tcatcccgct ggtggcgatc ctgccgatcc
     1201 tgctctacat cggcctggtg atcggcgcgc aggcgttcca ggcgagcccg tccaagcatg
     1261 cgcccgcggt ggtgctggcg atcatcccga acgtcgccga gtgggccaag atcagcatgg
     1321 acagcgcgct gcaggtcgcc ggcagcagcg ccgacaaggt cggcttcgct gcgctggcac
     1381 aggtgggggt ggtctatcag gggatggccc tcctcggggg cggatcggtg ctcgccggca
     1441 tggtgctcgg cgcgatggcc gtgttcatca tcgaccacaa gctcaagcac gccgcggccg
     1501 cggcgtttat cggagcggtg ctggccttcg tcgggctgat ccacgcgccg cagctcgcgt
     1561 tgggcgcatc gccgctggtg gcgctcggct acgcgatgtt cggcgcgctg tgcctggcgg
     1621 cagagctgac cgaccgcaaa gcgaccgcct gaaccgccgt gctaccgcag atcgcggccg
     1681 gtctcggcga cgaggtcggc cgctttctcg gccaccgcga tcaccgcggc gttgatgttg
     1741 cccgagggaa cggccggaat gaccgaggcg tccacgacgc gcaggcgctc gacgccctgc
     1801 acgcgaagct gcgggtcgac caccgcgcgc ccgtccgttc ccatgcggca ggtcgaggtc
     1861 gggtggaaca ccgtatcgcc ggcctcgcgg cagaaggcga gcagatcttc atcgctgcgc
     1921 acgcccggcc ccggatactc ctccgccacg acgtaatccc gcagcggcgg tgtgacggcg
     1981 atgcgccgca cgagcttcag accgtccacc atgacacggc ggtcgttctc ggttgagaga
     2041 tagttgtagc ggattgcggg cgcggcgcgc ggatcggccg aggcgatgtg caccgagccg
     2101 cgcgactcga cctgggtctg cgacaccgac agcgtgaagc cggagaacgg atgcagcgtg
     2161 ccgccgcgct tggcggtgga gaagttgatg aagtaaacct gcacgtccgg gcgcgcggcc
     2221 tgcggttgcg tgcgcacgaa cgccccggca tagccggcgc tgacggtgag cgggccgcgg
     2281 cgaaacagca ggtattgcag gccgatcttc gcctgacgcc agcggctcag catgtcgtcg
     2341 ttcagcgtga tcggtcggtt gcagcgccag aaagtgcggc agtagaaatg gtcctgcaga
     2401 tcctcgccca cgcccggcac atcgt
//
LOCUS       DASPGE010000265         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1124105, whole genome shotgun sequence.
ACCESSION   DASPGE010000265 DASPGE010000000
VERSION     DASPGE010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124105"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VD791_08600"
     CDS             complement(<1..877)
                     /locus_tag="VD791_08600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HXV08075.1"
                     /translation="MAHIVILGAGIGGLPMAYDMRKRARKEDRITVISNVDYFQFVPS
                     NPWVAVNWRKRDDIAFPIAPYLEKKDIGFISAGARRVHPERNQVELADGRVVDYDYLV
                     IATGPKLAFDEVEGLGPDAHTHSVCHVDHATAAGQAWEAFVQDPGPIVVGAVQGASCY
                     GPAYEFAMIMDTDLRRRKIRDRVPMTFVTAEPYIGHLGLGGVGDSKGLLESAMRDRHI
                     KWITNAKVTRIEAGKMHVTEFDELGKEKRTHELSFKYSMMLPAFKGIDAVLGIEGLTN
                     PRGFILVDKHQRNPKY"
     gene            complement(931..1977)
                     /locus_tag="VD791_08605"
     CDS             complement(931..1977)
                     /locus_tag="VD791_08605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV08076.1"
                     /translation="MRKTGMNSALAGMALAAAFGASAQSTRVAPVEYREVDTTYSAEA
                     VVEAVRQSTISAQVMGRVVELRVDAGDRVKAGQVIARIDEREAAQMVAGSEAQVAQAS
                     ADLENARLNLERSRRLLEQKFVSQSAVDRAESEFKAAQARLRAAQAGAGQAAVTRGYT
                     TVVAPFSGVISQRHVEVGEMAAPGKPLFTAFDPADMRVIAEVPQSRVADIRANARAMV
                     EVPSADLWIKVASMTVLPAADPRTHSTRVRLVLEDAGRAIYPGVYARAHFAVGRARKL
                     VVPAEAVLRRSEVTGVYVIDAKGKAQFRQVRLGEPAGDGYIEVLAGVSPSEKVSLEPV
                     KAGIASRTSRPSGS"
BASE COUNT          296 a          728 c          668 g          343 t
ORIGIN      
        1 ggtacttcgg gttgcgctgg tgcttgtcca ccaggatgaa gccgcgcggg ttggtgagcc
       61 cctcgatgcc gaggaccgca tcgatgcctt tgaacgcagg cagcatcatc gagtacttga
      121 acgacagctc gtgcgtgcgc ttctccttgc ccagctcgtc gaactcggtg acgtgcatct
      181 tgcccgcctc gatgcgagtg accttggcgt tggtgatcca cttgatgtgg cggtcgcgca
      241 tcgccgactc gagcagccct ttgctgtccc ccacgccgcc cagccccagg tggccgatgt
      301 agggctcggc ggtgacgaag gtcatcggca cgcgatcgcg gatcttccgg cgccgcaggt
      361 cggtgtccat gatcatggcg aactcgtagg ccgggccgta gcacgatgcg ccctgcaccg
      421 cgcccaccac gatcgggccc gggtcctgca cgaacgcttc ccacgcctgg ccggcggcgg
      481 tcgcatggtc cacgtggcac accgaatggg tgtgagcgtc gggccccagg ccttccacct
      541 cgtcgaaggc gagcttcggt ccggtggcga tcaccaggta gtcgtagtcc acgacacgcc
      601 cgtcggccag ctccacctgg ttgcgctccg gatgcacgcg cctggccccg gccgagatga
      661 agccgatgtc cttcttctcc agatagggcg cgatcgggaa ggcgatgtca tcgcgcttgc
      721 gccagttgac ggcgacccag ggattggacg ggacgaactg gaagtagtcg acgttggaga
      781 tgaccgtgat gcggtcctcc ttgcgggcgc gcttgcgcat gtcgtaggcc atcggcagcc
      841 cgccgatgcc agcgcccaga atgacgatgt gtgccatagg tgttggacct cgcagatgtg
      901 gaagttggaa gcggtcctcc agcgctgccg tcaagagccg ctcggcctgg acgtacggct
      961 ggcgatgccg gccttcaccg gctccagcga aaccttctcg ctcggcgaga cgcccgccag
     1021 cacttcgatg taaccgtccc cggcgggctc gcccagccgc acctgccgga attgcgcctt
     1081 gcccttcgcg tcgatgacgt acaccccggt gacttcgctg cgccggagca cggcttccgc
     1141 gggcaccacc agcttccggg cgcgccccac ggcgaaatgc gcgcgggcgt acacgccggg
     1201 gtagatggcg cggcctgcat cctcgagaac caggcgcaca cgcgtcgagt gcgtgcgcgg
     1261 gtcggccgcc ggaagcaccg tcatggaggc gaccttgatc cacagatcgg ccgagggcac
     1321 ctcgaccatc gcgcgcgcgt tggccctgat gtcggcgacc cgggactgcg gcacctcagc
     1381 gatgacgcgc atgtcggcgg ggtcgaaggc ggtgaatagc ggcttgcccg gcgcggccat
     1441 ctcgcccacc tcgacgtggc gctgcgagat cacgcccgag aacggcgcca ccaccgtggt
     1501 atagccgcgc gtgaccgccg cctggccggc gcccgcctgc gccgcccgca gccgcgcctg
     1561 cgcggccttg aattccgatt cggcgcggtc caccgccgac tggctgacga acttctgctc
     1621 gagcaaccgg cgcgagcgct cgaggttgag ccgtgcgttc tccagatcgg cgctcgcctg
     1681 ggccacctgc gcctcgctcc cggccaccat ctgcgccgct tcgcgctcgt cgatgcgggc
     1741 gatgacctgg ccggctttga cccggtcgcc cgcgtccacc cgcagctcca ccacgcggcc
     1801 catgacctgc gcggagatgg tggattggcg caccgcttcc accaccgctt ccgcggagta
     1861 ggtggtgtcc acctcgcggt actcgaccgg tgccacgcgc gtgctctgtg cactcgcgcc
     1921 gaacgcagcg gccagcgcca tccccgccag cgcactgttc atcccggttt tccgcatagc
     1981 cgctctctcc cgttccagca tgggacaacg atactgcgcc ccagttgagg acggt
//
LOCUS       DASPGE010000266         8185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_187406, whole genome shotgun sequence.
ACCESSION   DASPGE010000266 DASPGE010000000
VERSION     DASPGE010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8185
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187406"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VD791_08610"
     CDS             complement(<1..417)
                     /locus_tag="VD791_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXV08077.1"
                     /translation="MRRNVEFNSKGSKIRGVLVTPNGGGGPFPVAVMGGGWCYVKEIV
                     LPHYAEAIVKAGVAVLMFDYRGLGESEGEPRQHIDPWGQVEDYKNAISFAMKQREIDP
                     RRIGIWGISYAGGHVVIVGATDPRVKCIVSTVPVVEG"
     gene            complement(533..1684)
                     /locus_tag="VD791_08615"
     CDS             complement(533..1684)
                     /locus_tag="VD791_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV08078.1"
                     /translation="MQACARRPVAHCIGRTLLATLLLAPLGAAAQSKEPIKIGAVLAL
                     TGPGAGLGQSERNGIVLAEKLINQQGGIRGRPLQVLIEDDGSKADIAKSKAENLVFNH
                     KVKAILGPSLTASTGAVAAITNAQRMAQLAMTGLGPQIELTYKYLYHMLPPQTLNARA
                     MLEYTTKALKARKVGVLHDSGYGQLVTNALKEQSSAYAVEYLAIEKFEVGATDVSTQA
                     AKVRAANPEVVFIIATNPIPFRNARQMRIAQPIVSAIGSSAYQYVSGMGEFAHDIVFP
                     EFVVGEDPLPHQAEFVNLYRKAYNALPKNYEAAGWDAVHVLARALDKAGPDASPEAIA
                     MAVREPYKGAFAAYDFAAPDMTGIELSSYVYSKLVNGKFTRLAFTAKSP"
     gene            1928..2683
                     /locus_tag="VD791_08620"
     CDS             1928..2683
                     /locus_tag="VD791_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXV08079.1"
                     /translation="MKAGERQRMRAPRARAHPARPPARRLPRAERERAIIQHAVRFFA
                     EVGFGGDTRELAKRAHVTQALLFRYFHSKDALVERVYHEVYLGRWNPYWEMLIQDRSI
                     PLRERMVRFYRLYARTILSYEFVRLLMFAGLRGGTLTRRWFALVAERIVLPICREIRV
                     AHALPDFDRVPPTQTEVELVWSVNSRVFYYGVRKYIYGMPVPDNLDAMIEAEVHTFFD
                     GVASTLKDLFAHRASAPRGRRNRATPQRRGSWS"
     gene            2809..5430
                     /locus_tag="VD791_08625"
     CDS             2809..5430
                     /locus_tag="VD791_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXV08080.1"
                     /translation="MEQIKPAPAGSLRTTRRTAQHIVPADDPVLIGQVRLLHDNALLS
                     QLVALINSAILAYVLWPEVEQRRILAWLGCMVVACLLRLGEARAFARAQEPERQILVW
                     RNRFLLGAFTSGVLWGATGALLFPAQSLPHQVFVSFVVAGMVAGAVATLSPVMLAFAL
                     FALPALAPVVLQFLIHESPLHFPMSFMGVLFGLAMATVARHVNATVRENLSVSLHNVQ
                     LVEVLQRNNELTEKLNRRLLSEIDQRRTKENQRQRQQQLLESERRVMRAIAAGVPLPQ
                     AMELLCQTVEEQAPGALCAVLLLERDGDRLQRCAAPSLPDAYARALEIQHVGPASGFA
                     AAAAHLGRQMLVCDIEADVQWEECRALALREGLRACWSTPIPGGERAVLGVLDVYFRT
                     AREPTEECAELIARAADIARIAIERNEAERRIQQLAHYDALTGLPNRVMFGQALEQAL
                     HRAQRHDQPLALLFVDVDRFKNINDALGHDAGDQLLREVAQRLRQCLRASDLVARFGG
                     DEFIAVLEELPHGGYAGSVAAKLLQALAEPMRIQEQEFHVTASIGIAAFPQDAPDAIT
                     LQKHADIAMFRAKAQGRNGYCFYAPQGGAYSLERLTMEAQLKRALERDELVLHYQPKQ
                     DIATGRITGMEALLRWQHPELGLVSPARFIPLAEETGLIVPIGEWVLHTACAQAAALP
                     HGADGHALRVAVNLSARQFADDSLIALVARVLEQTGLASAALELEITETLIMQSPERT
                     ARLLAALREQGVKLAMDDFGTGYSSLAYLKRFPLDSIKIDRSFIHGVPGDGDGESITQ
                     AVIAMAHSLRLRAIAEGVETAQQLEFLRDHGCDEIQGYFFSRPLPYEELADLLTRLEA
                     APRVAPGARPRDFPFWP"
     gene            complement(5432..6388)
                     /locus_tag="VD791_08630"
     CDS             complement(5432..6388)
                     /locus_tag="VD791_08630"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HXV08081.1"
                     /translation="MTALARLEQAAAVVYAAMPATPQYRWPLLERALRTPVWVKHENH
                     TPVGAFKVRGGLAYFHALAQAGERPRGVVSATRGNHGQSVAFAAARYGLPATIVVPHG
                     NSVEKNDAMRALGADLIEHGHDFQAARDHATRLAGERGLLMVPSFHPLLVDGVASYSL
                     ELLRAVPELDVAYVPIGLGSGICGMIAARDALGLATDIVGVVSAQAPAYALSFEAGRP
                     IEHAADTRIADGVACRVPEPEALAILRRGCARVVRVTDDEVEAAMRLMFTATHNVAEG
                     AGAAPLAAALQERDRLAGRKLGLVLSGGNVDREVFARVLNAS"
     gene            complement(6416..7438)
                     /locus_tag="VD791_08635"
     CDS             complement(6416..7438)
                     /locus_tag="VD791_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXV08082.1"
                     /translation="MKLTEIPQATRIDIAAWVLMAVALWLVLKLHLLPSLLAGLLVYE
                     LVHLLAPRMRLWRLGSTWSKVAAVTLLSVIVVSLLTLGVFGVIALLRSELGSPGGLLK
                     ELAAIIDQSRDVLPAWALAKLPANADQLREAAAEWLRSNARAFQAFGTGFGRALVHIL
                     IGFVIGALISLREATPHREIGPLTQSLAERIGRLGEAFRRIVFAQVRISALNTTLTAI
                     YLAVVLPLFDVHLPLLKTMIAVTFIAGLLPVIGNLISNTVICIISLSHSLYVTIASLT
                     FLVVIHKLEYFVNARIIGTQIRSRAWELLIAMLVMEAAFGVAGVIAAPIFYAYLKDEL
                     KSRSMI"
     gene            complement(7445..>8185)
                     /locus_tag="VD791_08640"
     CDS             complement(7445..>8185)
                     /locus_tag="VD791_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXV08083.1"
                     /translation="RLDLDRLLDALTPQTRVLFVNSPNNPTGWTIGRTEQRAILEHCR
                     RHGIWLVADDAYERLCYDGDAGGGVAPSFLDLADPEDRVVSTNTFSKSWLMTGWRLGW
                     MRGPAALIAEMPKLIEYNTSCTPVFVQRAGMVAVSEGEPVVARTLTRLRQARDFLLEQ
                     LARTPGIEVARPAGAMYAFFRVAGVHDSLALCKHLVAGAGLGLAPGIAFGPEGEGFVR
                     WCFAASEARLAEGIERLRRGLATFARHA"
BASE COUNT         1260 a         2847 c         2824 g         1254 t
ORIGIN      
        1 cccctctacc accggcacgg tggacacgat gcacttcacg cgcgggtcgg tggcgcccac
       61 gatcaccacg tgcccgccgg cataggagat gccccagatg ccgatgcgcc tgggatcgat
      121 ctcgcgctgc ttcatcgcga agctgatggc gttcttgtag tcctccacct ggccccaggg
      181 gtcgatgtgc tggcgcggct cgccctcgct ctcgcccagc ccgcggtagt cgaacatcag
      241 caccgcgacg ccggccttga cgatcgcctc cgcgtagtgc ggcagcacga tctccttgac
      301 gtagcaccag ccgccgccca tgacggccac cgggaacggc ccgccgccgc cgttgggcgt
      361 gaccagcacg ccgcgaatct tcgaaccctt gctgttgaac tcgacgttcc tgcgcatctc
      421 cttctcctga gattagggtg aatcctccga tcgtcgctac tgccgacgaa gagccgtgat
      481 tcgtgaatcg tgattcgtac cccgacggcg ctcacgaatc acgagtcacg aattacggac
      541 tcttggccgt aaacgccagc cgggtgaact tgccgttcac cagcttggag tacacgtaac
      601 tggacagctc gatgccggtc atgtccggcg cggcgaagtc gtaggcagcg aacgcgccct
      661 tgtacggctc gcggacggcc atggcgatgg cctccggcga agcgtccggg ccggccttgt
      721 ccagcgcgcg ggcgagcacg tgcaccgcgt cccagccggc ggcctcgtag ttcttgggca
      781 gcgcgttgta ggctttgcgg tacaggttga cgaactcggc ctgatgcggc agcggatcct
      841 cgcccaccac gaactcgggg aacacgatgt cgtgcgcgaa ctcgcccatg ccgctcacgt
      901 attgataggc ggaggagccg atcgcggaca cgatcggctg ggcgatgcgc atctggcgcg
      961 cgttgcggaa cgggatcggg ttggtggcga tgatgaacac cacctccgga ttggccgcgc
     1021 gcaccttggc cgcctgcgtc gacacgtcgg tggcgccgac ctcgaacttc tcgatggcga
     1081 gatactccac cgcgtacgcc gaggattgct ccttgagggc gttggtgacg agctggccgt
     1141 agcccgagtc gtgcagcaca cccaccttcc tcgccttgag cgccttggtg gtgtactcca
     1201 gcatcgcccg ggcgttgagc gtctgcggcg gcagcatgtg gtacaggtac ttgtaggtga
     1261 gctcgatctg cggcccgaga ccggtcatgg cgagctgcgc catgcgctgc gcgttggtga
     1321 tcgccgccac cgctccggtg ctcgccgtca gcgacgggcc caggatcgcc ttgaccttgt
     1381 ggttgaagac caggttctcg gccttgctct tggcgatgtc ggccttggag ccgtcgtcct
     1441 cgatcagcac ctgcagcggc cggccgcgga tgccgccctg ctggttgatc agcttttcgg
     1501 ccaggacgat gccgttgcgc tcggactggc cgaggccggc tccgggaccg gtgagcgcca
     1561 gcaccgcacc gatcttgatg ggttccttcg attgcgccgc agcaccgagc ggcgccagta
     1621 gcagcgtggc gagaagcgtg cggccgatgc aatgcgcaac gggccgccgg gcgcaagcct
     1681 gcatatcctc cccctccgac gaacgcggtg cctggacgat gcgggcgtgg ggaatgctgt
     1741 tcttgggccc gcgccggtga tcttagtaag cagatgctca ccagtcaagc ggcccccggg
     1801 tggctggcgg cgggcggtct ccgcctgtaa gatcgctgcg cacggaacca ggggacgcac
     1861 ggagggtctc gtcggggcgc gcttgttgag caagtgctca ccatgcggcc gcggcacgag
     1921 cgatcgcatg aaggcaggcg aaaggcaacg gatgcgcgcg ccacgggcgc gggcgcatcc
     1981 cgcacgtccc ccggcccgcc gcctgccgcg ggcggagcgc gagcgcgcca tcatccagca
     2041 cgcggtgcgc ttcttcgccg aggtcggctt cggcggcgac acgcgcgagc tcgccaagcg
     2101 cgcgcacgtg acccaggcgc tgctgttccg ctacttccac agcaaggacg cgctggtcga
     2161 gcgcgtctac cacgaagtct acctggggcg ctggaacccc tactgggaga tgctgatcca
     2221 ggaccgctcg atcccgctgc gcgagcgcat ggtgcgcttc taccggctgt acgcccggac
     2281 catcctgagc tacgagttcg tgcgcctgct gatgttcgcc gggctgcgcg gaggcacgct
     2341 cacccggcgc tggttcgcgc tggtggccga gcgcatcgtg ctgccgatct gccgcgagat
     2401 ccgggtagcg cacgcgctgc cggatttcga ccgggtgccg ccgactcaga ccgaagtgga
     2461 gctggtgtgg agcgtgaact cgcgcgtgtt ctattacggc gtgcgcaagt acatctacgg
     2521 catgccggtg ccggacaacc tcgacgcgat gatcgaagcc gaggtgcaca cgttcttcga
     2581 cggtgtggcg agcacgctga aggatctgtt cgcgcaccgc gcctcggcgc cgcgcggcag
     2641 acgcaaccgg gcgacgccgc agcggcgcgg cagctggtct tgactttcga ctttcgactc
     2701 ctgatgccgc gttgacactg gttgaccatc gcgcgcgccg gcgtgagcac ggcggcgccg
     2761 tggcgcatgg cttgcttcgg atcgggccat tcaccagcgg ctacaggaat ggaacaaatc
     2821 aagccggcgc cggcgggttc gctgcgcacg actcgacgca ccgcccagca catcgtcccc
     2881 gcggatgatc ccgtcctgat cggccaggtg cgcctgctgc acgacaatgc gctgctgtcg
     2941 cagctggtcg ccttgatcaa cagcgccatc ctggcctacg tgctgtggcc cgaggtggag
     3001 cagcgccgca tcctcgcctg gctgggctgc atggtggtgg cgtgcctgct gcgcctgggc
     3061 gaggcgcggg cctttgcgcg cgcgcaggag cccgagcggc agatcctggt ctggcgcaac
     3121 cgcttcctgc tcggtgcctt cacttccggg gtgctgtggg gcgcgaccgg tgcgctgctg
     3181 ttcccggccc aatccctgcc gcaccaggtg ttcgtgtcgt tcgtggtggc cgggatggtg
     3241 gcgggggcgg tggccacgct ctcgccggtc atgctcgcct tcgcgctgtt cgcgctgcct
     3301 gcgctcgcgc cggtggtgct gcagttcctg atccacgaga gcccgctgca ttttccgatg
     3361 agtttcatgg gcgtgctgtt cggtctcgcc atggccacgg tggcgcgcca cgtgaacgcc
     3421 acggtgcgcg agaacctcag cgtgagcctg cacaacgtgc agctggtgga ggtgctgcag
     3481 cggaacaacg agctcaccga gaagctcaac cgccggctgc tgtccgaaat cgaccagcgc
     3541 cgcaccaagg agaaccagcg ccagcgccag cagcagctgc tggaaagcga gcgccgcgtg
     3601 atgcgggcga tcgccgccgg cgtgccgctg ccccaggcca tggagctgct gtgccagacg
     3661 gtggaggagc aggcgccggg cgcgctgtgc gcggtgctgc tgctcgagcg cgacggcgac
     3721 cggctgcagc gctgcgcggc gcccagcctg ccggatgcct acgcccgggc gctcgaaatc
     3781 cagcatgtcg gcccggcgag cggcttcgcc gctgccgccg cacacctcgg gcgtcagatg
     3841 ctggtgtgcg acatcgaggc cgacgtgcaa tgggaggagt gccgcgcgct ggccttgcgc
     3901 gaaggtctgc gcgcctgctg gtcgacgccg attcccggcg gcgagcgcgc ggtgctgggc
     3961 gtgctggacg tgtacttccg gaccgcacgc gagccgaccg aggagtgcgc cgagctgatt
     4021 gcgcgtgccg ccgacatcgc gcgcatcgcc atcgagcgca acgaggctga gcgccgcatc
     4081 cagcagctcg cccactacga cgctctcacc gggttgccca accgggtcat gttcggccag
     4141 gccctggagc aggcgctgca ccgcgcgcag cgccacgatc aaccgctggc gctgctgttc
     4201 gtggacgtgg accgcttcaa gaacatcaac gacgcgctcg gccacgacgc cggcgaccag
     4261 ctgctgcgcg aagtggcgca gcggctgcgc cagtgcctgc gcgcgagcga cctggtggcg
     4321 cgcttcggcg gcgacgagtt catcgccgtg ctggaggagt tgccgcacgg cggctacgcc
     4381 ggatcggtgg cggccaagct gctgcaggcg ctggccgaac cgatgcgcat ccaggagcag
     4441 gaattccacg tgacggcgag catcggcatc gccgccttcc cgcaggacgc gcccgacgcc
     4501 atcacgctgc agaagcacgc cgacattgcc atgttccgcg ccaaggcgca gggccgcaac
     4561 ggctattgct tctacgcgcc gcagggcggc gcctactcgc tcgagcggtt gaccatggag
     4621 gcgcagctga agcgcgccct cgagcgcgac gagctggtct tgcactacca gccgaagcag
     4681 gacatcgcca ccggccgcat taccggcatg gaggccctgc tgcgctggca gcatcccgag
     4741 ctgggtctgg tgtcgcccgc gcgcttcatt ccgctggcgg aggagaccgg gctgatcgtg
     4801 ccgatcggcg agtgggtgct gcataccgcc tgcgcccagg ccgcggcgct gccgcatgga
     4861 gcggacggcc acgcattgcg ggtggcggtg aacctgtccg cgcggcagtt tgccgacgac
     4921 agcctgatcg cgctggtggc gcgcgtgctg gagcagaccg ggctcgccag cgccgccctg
     4981 gagctggaga tcaccgagac gctgatcatg cagagtcccg agcgcaccgc acgcctgctg
     5041 gctgcgctgc gggagcaggg cgtgaagctg gcgatggacg acttcggcac cggctactcg
     5101 tcgctcgcct acctcaagcg cttcccgctg gacagcatca agatcgaccg ctccttcatc
     5161 cacggcgtcc cgggcgacgg cgacggcgag agcatcacgc aggcggtgat cgccatggcg
     5221 cacagcctgc gcctgcgcgc gatcgcggag ggcgtggaga ccgcgcagca gctcgagttc
     5281 ctgcgcgacc atggctgcga cgagatccag ggctacttct tcagcaggcc gctgccctat
     5341 gaggagctcg ccgacctgct gacccggctg gaggcagcgc cgcgcgtcgc gccgggtgcc
     5401 cggccgcgcg actttccgtt ctggccatag cctaactcgc gttcagtacc cgggcgaaca
     5461 cttcgcggtc gacgttgccg ccggacagca ccagaccgag cttgcggccg gcgaggcggt
     5521 cgcgctcctg cagggcggcg gcgagcggag cggcgccggc gccctcggcg acgttgtggg
     5581 tggcggtgaa catcagccgc atcgccgctt ccacttcgtc atcggtgaca cgcaccacgc
     5641 gtgcgcagcc gcggcgcaga atggcgagcg cctcgggctc gggcacgcgg caggccacgc
     5701 cgtcggcgat gcgcgtatcg gccgcatgct cgatcggccg cccggcctcg aacgacagcg
     5761 cataagccgg agcctgcgcg gacaccacgc cgacgatatc ggtggcgagc ccgagcgcgt
     5821 cacgcgccgc gatcatgccg cagatccccg agcccaggcc gatcggaaca taggccacgt
     5881 ccagctccgg caccgcgcgc agcagctcca acgaatagct cgccacgccg tccaccagca
     5941 gcgggtgaaa ggacggcacc atcagcaacc cgcgctcgcc cgccagccgc gtggcgtgat
     6001 cgcgggcggc ctggaagtcg tggccgtgct cgatcaggtc cgcgcccagc gcgcgcatgg
     6061 cgtcgttctt ctcgacgctg ttgccgtgcg gcaccacgat cgtcgccggc aggccgtagc
     6121 gcgccgcggc gaacgcgacc gactggccgt ggttgccgcg tgtggcgctc accactccgc
     6181 gcgggcgctc gcccgcctgc gcgagcgcgt ggaaatacgc gagccccccg cgcaccttga
     6241 acgcgcccac cggcgtgtgg ttctcgtgct tgacccatac cggagtgcgc agcgcccgtt
     6301 ccagcagcgg ccagcgatac tggggggtgg cgggcatggc cgcgtagacc acggcggcgg
     6361 cctgttccag gcgggcgagg gcagtcatcg ggatcgccac agttagggag cttccctaaa
     6421 tcatgctccg gctcttcagc tcgtccttca gataggcgta gaagatcggc gcggcgatca
     6481 cgccggccac gccgaaggcg gcctccatca ccagcatcgc gatcagcagc tcccaggcgc
     6541 gcgagcggat ctgcgtgccg atgatgcgcg cgttgacgaa gtattcgagc ttgtggatca
     6601 cgaccaggaa agtgagcgag gcgatcgtca cgtacagcga gtggctcagg ctgatgatgc
     6661 agatcaccgt gttcgagatc agattgccga tcaccggcag cagaccggcg atgaaggtca
     6721 ccgcgatcat ggtcttcagc agcggcaggt gcacgtcgaa cagcggcagg acgaccgcca
     6781 ggtagatcgc ggtcagggtg gtgttgagcg cggagatgcg cacctgcgcg aacacgatgc
     6841 gccggaaggc ctcgccgagc cgcccgatgc gctcggcgag cgactgggtg agcgggccga
     6901 tctcacggtg cggagtggct tcgcgcaacg agatcagcgc accgatgacg aacccgatca
     6961 ggatgtgcac cagcgcccgg ccgaagccgg tgccgaaagc ctggaacgcc ctggcgttgc
     7021 tgcgcagcca ctccgccgcg gcctcgcgca gctggtcggc gttggccggc agcttggcca
     7081 gggcccaggc cggcaacacg tcgcgcgact ggtcgatgat cgcggccagc tccttcaaca
     7141 gcccgcccgg gctgccgagc tcgctgcgca gcaacgcgat cacgccgaac acgcccagcg
     7201 tgagcaggct gaccacgatc accgacagca gcgtgacggc cgccaccttg ctccaggtgc
     7261 tccccagccg ccacagccgc atgcgtgggg cgagcaggtg caccagctcg tacaccagca
     7321 ggcccgcgag cagcgagggc aacaggtgca gcttgagcac cagccacagc gcgaccgcca
     7381 tcagcaccca ggcggcgata tcgatgcgcg tcgcctgtgg gatctcggtc agcttcatca
     7441 cggctcacgc atgccgtgcg aaggtcgcga gtccgcgccg caggcgttcg atgccttcgg
     7501 ccagccgcgc ctcgctcgcc gcgaagcacc agcgcacgaa accctcgcct tccggcccga
     7561 acgcgatgcc gggcgccagc cccaacccgg cgccggcgac cagatgcttg cacagcgcca
     7621 gactgtcgtg caccccggcc acgcggaaaa acgcatacat cgcgcccgcc ggccgcgcca
     7681 cctcgatgcc cggggtgcgc gcgagctgct ccagcaaaaa gtcgcgcgcc tgccgcaagc
     7741 gcgtcaacgt gcgcgctacc accggctcgc cctcgctcac cgcgaccatg ccggcgcgct
     7801 gcacgaacac cggtgtgcac gaggtgttgt actcgatcag cttgggcatt tccgcgatca
     7861 gcgccgccgg cccgcgcatc cagcccagcc gccagccggt catcagccag gacttggaga
     7921 acgtgttggt gctgaccacg cggtcttccg ggtccgccag gtcgaggaag gacggcgcca
     7981 ccccgccgcc ggcatcgccg tcgtagcaca ggcgctcgta ggcgtcgtcg gcgaccagcc
     8041 agatgccgtg ccggcgacag tgctccagga tggcgcgctg ctcggtgcgg ccgatggtcc
     8101 agccggtcgg gttgttgggc gagttgacga acagcacccg ggtctgcggc gtcagcgcgt
     8161 cgagaaggcg gtccaggtcc aggcg
//
LOCUS       DASPGE010000267         3541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1124672, whole genome shotgun sequence.
ACCESSION   DASPGE010000267 DASPGE010000000
VERSION     DASPGE010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124672"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..613
                     /gene="rnhA"
                     /locus_tag="VD791_08645"
     CDS             161..613
                     /gene="rnhA"
                     /locus_tag="VD791_08645"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HXV08084.1"
                     /translation="MSDSVEIYTDGACKGNPGPGGWGVVLRAGARVKELHGGEPGTTN
                     NRMELTAVIRALESLNRPCAVRLHTDSQYVQKGITEWIHAWKLRGWRTAGKKAVKNAD
                     LWRQLDELAGQHQIKWIWVKGHAGDPGNERADELANLGVEQVQHGPEV"
     gene            618..1352
                     /gene="dnaQ"
                     /locus_tag="VD791_08650"
     CDS             618..1352
                     /gene="dnaQ"
                     /locus_tag="VD791_08650"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HXV08085.1"
                     /translation="MRQIVLDTETTGLEVTLGHRVIELAAVEIVNRRFTGRTFHRYVN
                     PERDIDFGAVEVHGLTREFLQDKPRFGDVAREFLDFVQGAELIIHNAPFDVGFLDFEL
                     NLLDLPPIGEHCRPITDTLRMARDLYPGKRNSLDALCERYQVDNSARTRHGALLDAEL
                     LAEVYLAMTRGQETLVIDLALRGREAGEASFDLSTLDLIVLTATEAELAAHAQQIEAI
                     DKASNGACLWKRLEQSPALAGREFRA"
     gene            complement(1470..3062)
                     /locus_tag="VD791_08655"
     CDS             complement(1470..3062)
                     /locus_tag="VD791_08655"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HXV08086.1"
                     /translation="MAPAGSSAPEGLAGSSSQARFDAFLSYSSADRSLVRRIQRFLES
                     YRPPGRAQRLKVYLDETDMRGGSLPENLSAALADSRCLVVCWSDAAARSRWVRAETDE
                     FRKLGKEQSIAIVHVAGAGPMLKDQAFSGIEPIEHDLRRGWRSWFLTPKGKLELLRLI
                     AFLTGAEMRALRNWARRRALRNAAAPLALTALPVALLFVPLPAWDPVALRHRDEPITP
                     IGCEVVDGKLWVVSWDEGVGEISGARAFFTAYPDALSGAGEPIVRPRTFRLPKRALPH
                     DMATPALQKRFAAVLTEAGIDRSELAQRRDVRIAEPRRDHLLAILPQRHPEVDPQIAR
                     EAEKQGLPLPETDGSVVVVRQPGSAVKATQVNSLSPPLWNPRTEDRRLTPPARAISVA
                     WQDNGEIWIGVAGERKTPGGLWHSADAGQTWRRVDGFSSVTSIDLRSAARAQTVVVAE
                     QGIRQPRGTQLVSEPTRMMERTPDGAWTEIEAPPHDHDSEIEICGTVIDGLLYVRVGE
                     RLYGQGSRPLYRSVADVLGNRR"
     gene            complement(3125..>3541)
                     /locus_tag="VD791_08660"
     CDS             complement(3125..>3541)
                     /locus_tag="VD791_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV08087.1"
                     /translation="LDVLSSVLGVLTFAVAALGGISLVVGAVGILTIMTIAVAERTGE
                     IGLLRALGARREQVLLLFLAEAALLAAIGGLSGLALGIALAQLLHAVFPALPVTTPWS
                     YALAAEVVAVAIGVAAGVAPANRAARLDPVEALRAE"
BASE COUNT          568 a         1262 c         1205 g          506 t
ORIGIN      
        1 tgtacttcct gcacggcatc aagcgcgtgc acggcacgcg cgtcatcacc ccgcagtgga
       61 agagcagcct cgccaagagc aagcgctttg cgccggtgcc gcaccgcgtg caggacgacg
      121 agcgcctggt ggcgcgccag cgcgaccgcc acgccggcga gtgagcgaca gcgtcgagat
      181 ctacaccgac ggcgcctgca agggcaatcc cgggccggga ggctggggtg tggtgctgcg
      241 tgcgggcgcg cgcgtcaagg agctgcacgg cggcgagccc ggcaccacca acaaccgcat
      301 ggagctcacc gccgtcatcc gcgccctcga gtcgctgaac cgcccctgcg cggtgcgcct
      361 gcataccgac tcgcagtacg tgcagaaggg catcaccgag tggatccacg cctggaagct
      421 gcgtggctgg cgcaccgccg gcaagaaggc ggtgaagaac gccgatctgt ggcgtcagct
      481 cgacgagctg gccgggcagc accagatcaa gtggatctgg gtcaagggcc acgccggcga
      541 tccgggcaac gagcgtgcgg acgagctggc caatctcggc gtggagcagg tccagcacgg
      601 gcccgaggtc tgagggcatg cgccagatcg tgctggacac cgaaaccacc ggcctggagg
      661 tcacgctggg ccatcgcgtc atcgagctgg ccgcggtcga gatcgtcaac cgccggttca
      721 ccgggcgcac cttccaccgc tacgtcaacc ccgagcgcga catcgatttc ggtgccgtcg
      781 aggtgcacgg gctgacccgc gagttcctgc aggacaagcc gcgcttcggc gacgtggcgc
      841 gcgagttcct ggatttcgtg cagggcgccg agctgatcat ccacaacgcg ccgttcgacg
      901 tgggcttcct cgatttcgag ctgaatctgc tcgacctgcc gccgatcggc gagcactgcc
      961 ggccgatcac cgacactctg cgcatggcgc gcgacctgta ccccggcaag cgcaacagcc
     1021 tcgacgcgct atgcgagcgc taccaggtgg acaactccgc gcgcacccgg cacggcgcgc
     1081 tgctcgacgc ggagctgctg gccgaggtgt acctggcgat gacccgcggc caggagacgc
     1141 tggtcatcga cctcgccttg cgtgggcgcg aggcgggcga agcgtcgttc gatctgtcga
     1201 cgctggatct gatcgtgctc accgccaccg aagcggagct cgccgcgcac gcgcagcaga
     1261 tcgaggccat cgacaaggcg agcaacggcg cctgcctgtg gaagcggctg gaacaatcgc
     1321 cggcgctggc aggccgcgaa ttccgcgcgt aaactgagtc gagggcatgc cgctgtccga
     1381 ggtctatttc aacgtctccg gcctcggcag cagcgctgcc tgactgggac gacatcgagt
     1441 gggactggga gacgccctga gccccgtcct taacgccgat tacccagcac gtcggcgacc
     1501 gatcgataga gcggccggct gccctgcccg tagagccgct cgccgaccct gacatacagc
     1561 aggccgtcga tgacggtccc gcagatctcg atttccgaat cgtggtcgtg cggcggggcc
     1621 tcgatctcgg tccaggcgcc atccggcgtg cgctccatca tgcgcgtggg ctcggacacg
     1681 agctgcgtgc cacgcggctg ccggatgccc tgctcggcga ccacgacggt ctgagctcgc
     1741 gccgcgctgc gcaggtcgat cgacgtcaca ctggaaaagc cgtccacccg ccgccaggtc
     1801 tgtccggcgt cggcactgtg ccatagaccg ccgggtgtct tccgttcccc ggcgacgccg
     1861 atccagatct caccgttatc ctgccaggcg acggagatcg cccgcgccgg cggcgtcagg
     1921 cgcctgtctt ccgtgcgtgg gttccacagc ggcggtgaaa ggctgttcac ttgggtcgct
     1981 ttgaccgccg agcccggctg gcggaccacc accacgctgc cgtcggtttc cggcaacggc
     2041 agaccctgct tttccgcctc ccgggctatt tggggatcga cttccggatg gcgctgcggc
     2101 aggatcgcca ggaggtggtc tcgacgcggc tcggcgatgc gtacatctct tcgttgcgcc
     2161 aactcgctgc ggtcgattcc cgcttcggtc agcacggccg cgaaccgctt ctgcagcgca
     2221 ggggtcgcca tgtcgtgcgg cagcgcgcgc ttcggcagcc ggaaagtgcg cgggcggacg
     2281 atcggctccc cggcgccgct caacgcgtcc ggatacgccg tgaagaatgc ccgcgcgccg
     2341 gagatctcgc cgacgccttc gtcccaggac accacccaga gcttgccgtc cacgacctcg
     2401 catccgatcg gcgtgatcgg ctcatcgcga tggcgcagcg ccacgggatc ccaggcagga
     2461 agcggcacga acagcaaggc caccggcagc gcggtgagcg cgagcggcgc ggccgcgttg
     2521 cgcaaagcgc ggcggcgtgc ccagttgcgc aaggcgcgca tctcggcgcc ggtcaggaat
     2581 gcgatcaggc gcagcagctc gagcttgccc ttcggtgtca ggaaccagct ccgccacccg
     2641 cgtcgcagat cgtgctcgat cggctcgatc ccgctgaacg cctggtcctt cagcatcggg
     2701 cccgcgcccg ccacgtgcac gatcgcgatc gactgctctt tgcccagctt gcggaactcg
     2761 tcggtctcgg ctctgaccca tctcgaccgg gcggccgcgt cggaccagca caccaccagg
     2821 cagcgggaat cggccagggc ggcggacagg ttctccggca gagagccgcc gcgcatgtcg
     2881 gtctcgtcca ggtagacttt caggcgctgc gcccgaccgg gcggccgata gctttcgagg
     2941 aagcgctgga tgcggcgcac cagggaacgg tcggcgctgc tgtaactcag gaacgcatcg
     3001 aagcgcgcct gcgacgagct gccggccaac ccttccggcg ccgaggaccc tgctggggcc
     3061 atcgcgtcac tcctggatgg actgcggcgc agagcttagc gcagttctcg ccgcaccatg
     3121 ctgctcactc cgctctgagc gcctccaccg ggtcgaggcg cgccgcgcgg ttggcgggcg
     3181 ccacgccggc ggcgacgccg atcgcaaccg cgacaacctc cgcagccagc gcgtagctcc
     3241 acggtgtggt caccggcagg gcggggaaca ccgcgtgcag cagctgcgcc agcgcgatgc
     3301 ccagcgccag ccccgacagc ccgccgatcg ccgccagcag cgcggcttcg gccaggaaca
     3361 gcagcagcac ctgctcgcgg cgggcgccga gcgcgcgcag caggccgatc tcgccggtgc
     3421 gctcggccac tgcgatggtc atgatggtga ggatgcccac ggcgcccacc accagcgaga
     3481 tgccgccgag cgcggccacg gcgaaggtga gcacccccag caccgaggac agcacatcga
     3541 g
//
LOCUS       DASPGE010000268         3417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_187754, whole genome shotgun sequence.
ACCESSION   DASPGE010000268 DASPGE010000000
VERSION     DASPGE010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187754"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..983
                     /locus_tag="VD791_08665"
     CDS             114..983
                     /locus_tag="VD791_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV08088.1"
                     /translation="MDKTPLWPAQLDHIRLDSERPAALVEFYRAALGMAGTALEDGTV
                     LMQAPERRILIGPGQRAEQPFMAFRVQSRGQLEAFRRHVLAQGLEPLASPSAIFGRDA
                     FAVRDPDGRLAVFGLPRADLPPCMPADSLPAAKLPGRLQHVVVASSALPAMLRFYEEE
                     LGFVASDYVYQDAAQREPTVGFFRSDPEHHSFAVFRCPQTRPDHHSYETRCWNDIRDW
                     ADHLASLNVPLWWGPGRHGPGNNLFFMIQDPQGYLIEISAELEVVPDAVAKRAWKHEE
                     RTLNLWGSAFMRS"
     gene            987..2153
                     /locus_tag="VD791_08670"
     CDS             987..2153
                     /locus_tag="VD791_08670"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HXV08089.1"
                     /translation="MSAAPIIVVGGGIGGLAAAIALARQRIPTVVVERAPRFAEIGAG
                     IQLGPNVWKIFARLGLTERARRLAVFPRRLVLKDGLTAEEIGTVPCGAPLEEMFGHPY
                     ALIHRADLHRVLLDECQADALITLAPGTHLERFEQAPHAVTAWASDGRQWTGPALIGA
                     DGLWSQTREALLADGKPRVSGHIAYRAVLPYADAPAYAKEQAMTIFCGPRFHLVHYPL
                     RSGELLNLVAVFHSQRYDEGWDAFGDPAELNERFAQAHGNVRGLLERIDSWRMWVLCD
                     REPIAVWGKGRATLLGDAAHPMLQYLAQGAGMAIEDAWVLAECVASAGDMPEALREYE
                     RRRYLRTARCQIMARVYGEVFHATGVARELRNQLLSQRSPAQALAGMDWLYRGV"
     gene            complement(2179..2562)
                     /gene="pcaC"
                     /locus_tag="VD791_08675"
     CDS             complement(2179..2562)
                     /gene="pcaC"
                     /locus_tag="VD791_08675"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HXV08090.1"
                     /translation="MNDPLFEKGLKVRREVLGAEYVDRAIQTADDFNRPMQELVTRYC
                     WGEIWNRPGLDRKTRSMLNLAMLTALNRPHEIKMHVRGALNNGLTRAEIGEVFLHAAI
                     YCGVPAAIDSFRTAKEVFAELDAKK"
     gene            2692..3390
                     /locus_tag="VD791_08680"
     CDS             2692..3390
                     /locus_tag="VD791_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV08091.1"
                     /translation="MRFDDQVVAVTGANGNLGRALAQAALAQGAKLALLDVALGADLA
                     TSAPGRVGLHQVNLLDAEATRACFAGIGRVDVLCNIAGGFTMGDPVHAAGDEVWTRMF
                     DLNVRTLLNSVRAVVPGMIERKRGRIVNVGAIAGLGGGLSGQANMGAYSAAKSAVIRI
                     TESMAAELKRKGINVNCVLPSIIDTPQNRAAMSKADPAQWVAPADLANVILFLASEEA
                     RAVHGAALPVVNLV"
BASE COUNT          521 a         1142 c         1193 g          561 t
ORIGIN      
        1 agccctggcc ctgtctccca acgaaatcgc ggcggcgatc gcgctcgagt cgcgccggta
       61 cggcgatatc gtcaagcgtg cgaagatctc cctggactga tccgctcacg atgatggaca
      121 aaacgccact gtggccagcg cagctggatc acatccgcct cgacagcgag cgtccggctg
      181 cgctggtcga gttctatcgc gccgcactgg gcatggccgg gacggcgctg gaagacggga
      241 cggtgctgat gcaggcgccc gagcggcgca ttctgatcgg accggggcaa cgggccgagc
      301 agcccttcat ggcgttccgg gttcagagcc gggggcagct cgaagccttc agacggcatg
      361 ttctggcgca aggactggag ccgctcgcga gcccgagcgc gatcttcggc cgcgacgcgt
      421 tcgcggtgcg cgacccggac ggacgactgg ccgtattcgg gctgccgcgc gccgacctgc
      481 cgccttgcat gcctgcggac agcctgccgg ccgcgaagct gccggggcgt ctgcagcacg
      541 tcgtcgtggc gtccagcgcg ctgcctgcga tgctgcgctt ctacgaggag gaattgggct
      601 tcgtggcttc cgactacgtg taccaggacg ccgcgcaacg cgagccgacc gtgggcttct
      661 tccgctccga tcccgagcac catagctttg cggtgtttcg ttgcccgcag acccggcccg
      721 atcatcactc ctacgagacc cgttgctgga acgacatccg cgactgggcg gaccacctgg
      781 cgagcctcaa cgttccgctg tggtggggac ctggacgtca cggccccggc aacaacctgt
      841 tcttcatgat ccaggatccg cagggctatc tgatcgagat ctcggccgag ctggaggtgg
      901 tgcccgacgc ggtcgccaag cgggcctgga agcacgagga gcgcaccctg aatctatggg
      961 gctcggcctt catgcgaagc tgaagcatga gcgcggcacc catcatcgtc gtcggcggcg
     1021 gcatcggcgg cctggccgcg gcgatcgctc tcgcccgcca gcgcatcccg accgtggtcg
     1081 tcgaacgcgc gccgcggttc gccgagatcg gcgccggcat ccagctcggc cccaacgtgt
     1141 ggaagatctt cgcccggctc gggctcacgg agcgggcgcg cagactggcg gtattccccc
     1201 gccgactggt gctgaaggac ggcctcacgg cggaggagat cggcacggtc ccgtgcggag
     1261 ctccgctgga ggagatgttc ggccatccct acgcgctgat ccatcgtgcg gatctgcatc
     1321 gcgtgctgtt ggacgaatgc caggccgatg ccttgatcac gctggctccc gggacccatc
     1381 tcgagcgctt cgagcaggcc ccgcacgcgg tgacggcatg ggcaagcgac gggcggcagt
     1441 ggaccgggcc tgccctgatc ggcgccgacg gtctgtggtc gcagacccgg gaagcgttgc
     1501 tcgcggacgg gaagccgcgt gtgtcgggtc acatcgccta tcgagctgtc ctgccttatg
     1561 ccgacgctcc cgcgtatgcg aaggagcagg cgatgaccat cttttgcggc ccgcgcttcc
     1621 atctggtcca ctatccattg cgcagcgggg agctgttgaa cctggtggcc gtgtttcaca
     1681 gccagcgcta cgacgagggc tgggacgcgt tcggcgaccc ggcagagctg aacgagcgct
     1741 tcgcgcaagc gcatggcaac gtgcgcggcc tgctcgaacg catcgactcc tggcgcatgt
     1801 gggtgctctg cgaccgggag ccgatcgcgg tgtgggggaa gggccgggcg acgctgctcg
     1861 gcgatgcggc ccacccgatg ctccagtatc tcgcccaggg tgccggcatg gcgatcgagg
     1921 atgcctgggt actggccgag tgcgtggcga gcgccggcga catgcccgag gcgctgcgcg
     1981 agtacgagcg ccggcgctat ctccgcaccg cacgctgcca gatcatggcg cgcgtgtacg
     2041 gagaagtttt ccacgcgacg ggggtggcgc gcgagctgcg caaccagttg ctttcgcaac
     2101 gcagcccggc ccaggcgctg gccgggatgg actggctcta ccgaggagtg tgaaggttgg
     2161 ccggcctatc gggcggcgct acttcttcgc gtccagctcg gcgaacactt ccttggcggt
     2221 gcggaagctg tcgatcgccg ccggcacgcc gcaatagatc gcggcgtgca ggaacacctc
     2281 gccgatctcg gcgcgcgtca ggccgttgtt cagcgccccg cgcacgtgca tcttgatctc
     2341 gtgcggccgg ttgagcgcgg tgagcatggc caggttcagc atgctgcgag tcttgcgatc
     2401 caggcccggc cggttccaga tctcgcccca gcagtagcgc gtgaccagct cctgcatcgg
     2461 gcggttgaag tcgtcggccg tctggatcgc gcgatccacg tattcggcgc ccaacacctc
     2521 gcggcgcact ttcaggcctt tctcgaacag cggatcgttc atggcgcgct cctctcctgg
     2581 ggttgcgtgg aaagggcgca atcttgacag aaccgcgcgc ggcctgcagg gcacggtgga
     2641 tacgctacca tgcacggcgc ggcatgacga agatccgcgc gggagagatc gatgcgattc
     2701 gacgatcaag tggtggcggt gaccggcgcc aacggcaacc tcggccgcgc gctggcgcag
     2761 gcggccctgg cgcaaggggc gaaactggcg ctgctcgatg tcgctctcgg ggcggatctg
     2821 gccacgtccg cgcccgggag ggttggcctt caccaggtca acctgctcga cgccgaggcc
     2881 acacgcgcct gcttcgccgg catcggccgc gtcgacgtgc tgtgcaacat cgccggcggc
     2941 ttcaccatgg gtgacccggt gcacgccgcc ggcgacgagg tctggacgcg catgttcgat
     3001 ctcaacgtgc gtacgctgct gaacagcgtg cgcgccgtgg tccctggcat gatcgagcgc
     3061 aagcgcggca ggatcgtcaa cgtcggcgcc atcgccggcc tgggcggcgg gctcagcggc
     3121 caggccaaca tgggcgccta ttcggcggcc aagagcgcgg tgatccgcat caccgagagc
     3181 atggcggcgg agctcaagcg caagggcatc aacgtcaact gcgtgctgcc cagcatcatc
     3241 gacacgccgc agaaccgcgc cgcgatgtcg aaagccgatc ctgcacagtg ggttgccccg
     3301 gcggatctgg ccaacgtgat cctgttcctg gcgtcggagg aagcgcgcgc ggtgcacggc
     3361 gccgcgctgc cggtcgtgaa tcttgtctag cgccgcctcc tcggtcctag gccgcct
//
LOCUS       DASPGE010000269         3156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_891068, whole genome shotgun sequence.
ACCESSION   DASPGE010000269 DASPGE010000000
VERSION     DASPGE010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891068"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /gene="cysD"
                     /locus_tag="VD791_08685"
     CDS             <1..733
                     /gene="cysD"
                     /locus_tag="VD791_08685"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HXV08092.1"
                     /translation="TLLHIDTEHNFPEVIAFRDRTARERGLRLLVRSVGESIRTGRVR
                     LRDPGEPRNPHQSVTLLDAIAELGFDACIGGARRDEEKARAKERVFSFRDEFGQWDPK
                     NQRPELWAQYNGRVRQGENIRVFPLSNWTELDVWQYIRRERVELPSIYFAHRRPVVRR
                     GTALVPVTELTPARPGDTIETLSVRFRTVGDITCTAPVESFAATLDDIVRETQETRIS
                     ERGATRLDDQTNDSAMELRKKEGYF"
     gene            733..2088
                     /locus_tag="VD791_08690"
     CDS             733..2088
                     /locus_tag="VD791_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXV08093.1"
                     /translation="MAPANLALAPVSAPIPAQIAFAVQRTGLLRFITAGSVDDGKSTL
                     IGRLLYDSKGLLADHVTALEAASRRRGLSAIDLSLLTDGLLAEREQGITIDVAYRYFA
                     TPARKFIIGDSPGHAQYTRNMVTAASTADVAVLLVDARHGLQPQTRRHAYLARWVGIP
                     RVVLAVNKMDLVDGSQPAFEAIRQQFHRFATGLGFEEVREIPLSALRGDMVVERGEAL
                     AWYDGPTLLEWLETVPARHDETGKNGRQQPLRFPVQRVVRVAGGNAQPGSGFDGREFR
                     GYQGTVATGRLAVGDEVIALPAGTRARVAEILTADGPHDEAVADMAVTVRLDAELDIS
                     RGDLLASAATAPRLARDIDAEVCWFDGEPLAAGRPYLIKHGGATVRARFTALEHRIDV
                     DTLRLVERPESLAMNDIARVRITAQRPLAFDAYRDNRVTGAFIVIDETTNRTVAAGTI
                     A"
     gene            2085..2840
                     /gene="cobA"
                     /locus_tag="VD791_08695"
     CDS             2085..2840
                     /gene="cobA"
                     /locus_tag="VD791_08695"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HXV08094.1"
                     /translation="MTGTVYLVGAGPGAADLLTVRAARLLEQADIVFHDALVHPDTLA
                     LARNAVLVAVGKRCGRLSTAQRFINKQLIDAAARYRTVVRLKGGDPMLFGRAQEEIDA
                     LRAAGVAVEIVPGVSAAFGASADLAQSLTERGLSRSVVFVTPRAGEGETQHDWARPAA
                     AADTVVLYMAGKQAQAIRDSLLHAGLPASRPAVFVENASLPAQRVVPARLGELIEAAA
                     LLGDGPALLVVGEVYESIVAAAERETNALRRTA"
     gene            2922..>3156
                     /locus_tag="VD791_08700"
     CDS             2922..>3156
                     /locus_tag="VD791_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HXV08095.1"
                     /translation="MTTGLILFAHGARDARWREPFDRLQALVAQRHPGPVSLAFLELM
                     SPDLPQAATQLAAAGATRLVVVPLFLGTGGHLRQ"
BASE COUNT          461 a         1140 c         1112 g          443 t
ORIGIN      
        1 caccctgctg cacatcgaca ccgagcacaa cttccccgaa gtgatcgcgt tccgcgaccg
       61 caccgcgcgc gagcgggggc tacggctgct ggtgcgctcg gtgggcgaat cgatccgcac
      121 cggccgcgtt cgcctgcgcg atccgggcga gccgcgcaat ccgcatcagt cggtcacgct
      181 gctcgacgcc atcgccgagc tgggtttcga cgcctgcatc ggcggcgcgc gccgcgacga
      241 agagaaggcg cgcgccaagg agcgggtgtt ctccttccgc gacgagttcg gccagtggga
      301 cccaaagaac cagcgcccgg agctgtgggc gcagtacaac ggccgcgtgc gccagggcga
      361 gaacatccgc gtgttcccgc tctccaactg gaccgagctg gacgtgtggc agtacatccg
      421 ccgcgagcgg gtggagctgc cgagcatcta cttcgcgcac cggcggccgg tggtgcggcg
      481 cggcaccgcg ctagtgccgg tcaccgaact cacgccagcg cgtcccggcg atacgatcga
      541 aaccctgagc gtgcgtttcc gcacggtggg cgacatcacc tgcacggcgc cggtggagtc
      601 gttcgcggcg acgctggacg acatcgtgcg cgagacgcag gagacgcgca tcagcgagcg
      661 cggtgcgacg cgcctggacg accagaccaa cgactcggcc atggagctgc gcaagaagga
      721 aggctacttc tgatggcacc cgccaacctc gcccttgcgc ccgtatccgc gccgatcccg
      781 gcacagatcg cgttcgccgt gcagcgcacc ggactgctgc gcttcatcac cgccgggagc
      841 gtcgacgacg gcaagagcac gctgatcggg cgcctgttgt acgacagcaa gggcctgctg
      901 gcagaccacg tgaccgcgct ggaagcggcc tcgcgccggc ggggcttaag cgccatcgac
      961 ttgtccctgc tcaccgacgg tctgctcgcc gagcgcgagc agggcatcac catcgacgtg
     1021 gcctaccgct acttcgccac gccggcgcgc aagttcatca tcggtgactc gcccggtcat
     1081 gcgcagtaca cgcgcaacat ggtgaccgcc gccagcaccg ccgacgtggc ggtgctgctg
     1141 gtggatgcgc gccacggtct gcagccccag acccggcgcc acgcctatct ggcccgctgg
     1201 gtcggcatcc cgcgcgtggt cctggcggtg aacaagatgg acctggtgga cggctcgcaa
     1261 ccggccttcg aggcgatccg ccagcagttc caccgtttcg ccaccggcct cggcttcgag
     1321 gaggtgcgcg agatcccgct ttcggccttg cgcggcgaca tggtggtgga acgcggtgaa
     1381 gcgctggcct ggtacgacgg cccgacgctg ctggaatggc tcgagaccgt gcccgcgcgc
     1441 cacgacgaga ccggcaagaa cgggcggcag cagcccctgc gcttcccggt gcagcgcgtg
     1501 gtgcgcgtgg cgggcggcaa cgcgcaaccc ggctcgggtt tcgacggccg cgagttccgc
     1561 ggctaccagg gcacggtcgc aacggggcgg ctggccgtgg gcgacgaggt gatcgcgctg
     1621 cccgctggca cgcgcgcgcg tgtggcggag atcctgaccg ccgacggccc gcacgacgag
     1681 gcggtggccg acatggcggt cacggtgcgg ctggacgcgg agctggacat ctcgcgcggc
     1741 gacctgctgg cctcggcggc caccgcgccg cggctcgccc gcgacatcga cgcagaggtg
     1801 tgttggttcg acggcgagcc gcttgcagcc gggcgcccct atctgatcaa gcatggcggg
     1861 gccacggtgc gcgcccgctt caccgcgctc gagcaccgca tcgacgtgga caccctgcgg
     1921 ctggtggagc ggcccgagtc gctcgcgatg aacgacatcg cgcgcgtgcg catcaccgct
     1981 cagcggccgc tggcgttcga cgcctatcgc gacaaccgcg tcaccggcgc cttcatcgtg
     2041 atcgacgaga ccaccaaccg caccgtcgcc gcggggacga tcgcatgacg gggaccgtgt
     2101 acctggtcgg agccgggccg ggagcggccg acctcctcac cgtgcgcgcc gcgcggctgc
     2161 tggagcaggc cgacatcgtc ttccacgacg ccttggtgca ccccgacacc ctggcgctcg
     2221 cgcgcaacgc cgtgctggtg gcagtgggca agcgctgcgg caggctctcc accgcgcagc
     2281 gcttcatcaa caagcaactg atcgatgcgg ctgcgcgcta tcgcacagtc gtgcggttga
     2341 agggcggcga tccgatgctg ttcggccgcg cgcaggagga gatcgacgcg ctgcgcgccg
     2401 caggggttgc cgtcgagatc gtgcccggcg tgagcgccgc cttcggcgcg agcgccgacc
     2461 tcgcgcagtc gctgaccgag cgcggcctga gccgcagcgt cgtcttcgtc accccgcgtg
     2521 caggcgaggg cgaaacgcag cacgattggg cgcggcccgc tgccgccgcc gacaccgtcg
     2581 tgctgtacat ggccggcaag caggcgcagg ccattcgcga tagcctgctg cacgcgggtc
     2641 tgcccgcatc ccggccggcg gtgttcgtcg aaaacgcttc gcttcccgcg caacgcgtgg
     2701 tgcccgcacg cttgggcgag ctgatcgagg ccgccgcgct cctcggcgac ggcccggcgt
     2761 tgctggtcgt gggtgaggtg tacgaaagca tcgtcgccgc tgccgagcgc gaaaccaacg
     2821 cactgcgtcg caccgcctaa gggaggcgcg aagcgcccca ccgctccctc tccctcgacg
     2881 ggagagggct ggggtgaggg tggaggcgcg aagccctagc catgaccacg ggcctcatcc
     2941 tcttcgccca cggtgcgcgc gacgcgcgct ggcgggaacc cttcgaccgg ctgcaggcgc
     3001 tggtcgcgca gcgccatccc ggcccggttt cgctcgcttt cctcgagctg atgtcgcccg
     3061 acctgccgca agcggccacc cagctcgccg cggccggcgc aacgcggctc gtcgtcgtgc
     3121 cgctgttcct cggcaccggc ggccatctgc gccagg
//
LOCUS       DASPGE010000270         2504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1711105, whole genome shotgun sequence.
ACCESSION   DASPGE010000270 DASPGE010000000
VERSION     DASPGE010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711105"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VD791_08705"
     CDS             complement(<1..274)
                     /locus_tag="VD791_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HXV08096.1"
                     /translation="MQASVESQGADGEPRAARLSDATRRYFNEIGRRRLLSAEEERLC
                     ARRAHAGDFDARQRMIQHNLRLVVNIAKHYVGRGLSLLDLIAEGNLG"
     gene            complement(445..1356)
                     /locus_tag="VD791_08710"
     CDS             complement(445..1356)
                     /locus_tag="VD791_08710"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HXV08097.1"
                     /translation="MQNPSAAPACDAFISHASEDDALATRISEHLADNDISVWLDHQD
                     LRRKGLLLEALQRAIERCGHVVLLWSKDSAGSRYVSAEWNFAWNREHSIVPCRVDETK
                     LPLGLAGYLYCDFRSSFEGGAGQLRDALRGPAPAPARREAATLVRSAAFKKIYAQQDA
                     VLDALGRGDLARAKKLQLKLDPIVASAQRQFAQDADILGLAGYHCKNAYQIKHWAAIQ
                     ARQSPSDPLLAEAEARFWEALKCRPNDAGALNGLGSVLWLRGDLDAAEFYVERSIERA
                     RQEGFSYPYAEEDLQNIRREKALRKQR"
     gene            complement(1356..2228)
                     /locus_tag="VD791_08715"
     CDS             complement(1356..2228)
                     /locus_tag="VD791_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXV08098.1"
                     /translation="MRASAEAEALPEFYTVRKGDTLYSIALDNGQDYKDVAAWNGLTD
                     ANMIRVDQQLRVRPPPGWKDPAEEEGAVVARAVDAPPEIQAKPLEAPVVKKAPKALKV
                     RYSEQALAQMRGAPTKPASEPAPRPASPPSAQAGPPQAAPSTARAKPVTPPAAPASVS
                     ATASAGAITWAWPARGALLQPFNQGPNPKGIAIGGETGQPVLASAAGRVVYSGSGLRG
                     YGKLVIIKHDATYLSVYAHNRELRVKEGERVAAGQAIAEMGSFDAERPGLHFEIRRLG
                     KPVDPLQYLPARGG"
BASE COUNT          334 a          883 c          870 g          417 t
ORIGIN      
        1 ggccgagatt gccttccgcg atcagatcga gcagcgacag gccgcggccg acgtagtgct
       61 tggcgatgtt caccaccagc cgcagattgt gctggatcat gcgctggcgg gcgtcgaagt
      121 ctccggcgtg cgcacgccgc gcgcacaggc gctcctcctc ggcgctgagc aggcgccggc
      181 gcccgatctc gttgaagtag cggcgggtag cgtcggacaa tcgcgccgct cgcggctcgc
      241 cgtctgcgcc ctggctctcg accgaagcct gcagcgcatc gtccggttcg gcctcctcgg
      301 cgcgatcatc gagcgaactc acgccctctc ctccctgcag gcttcctctc attcgaggtc
      361 gcgtcgcgcc cgatgataac caacttgccg tgaccgcgca gcccctggcc gccgtgcacc
      421 ggcccgcttg ctcggcgccg ccggctatcg ctgcttgcgc agcgccttct cgcgccggat
      481 gttctgcaga tcttcttcgg cgtaaggata cgagaaccct tcctgccgcg cgcgttcgat
      541 ggagcgctcc acatagaact cggcggcgtc gagatcgccg cgcagccaaa gcacgctgcc
      601 caggccgttc aacgcgccgg cgtcgttggg gcggcacttg agcgcttccc agaagcgcgc
      661 ctcggcctcg gcgagcaacg gatcggaggg cgactggcgc gcctggatcg cagcccagtg
      721 cttgatctgg taggcgttct tgcaatgata gccggcgagt cccaggatgt cggcgtcttg
      781 cgcgaactgc cgttgcgccg aggcgacgat cggatcgagc ttgagctgga gtttcttcgc
      841 tcgggcgagg tcgccccggc cgagcgcgtc gagtaccgca tcctgctgcg catagatctt
      901 cttgaacgcc gcgctcctga cgagcgtggc cgcctcgcgc cgcgcgggtg ccggcgccgg
      961 cccgcgcaga gcatcgcgca attgtccggc gcccccctcg aaggaagagc ggaagtcgca
     1021 gtacaaatag ccggcgagcc cgagcggcag cttggtttcg tcaacccggc aggggacgat
     1081 ggagtgctcc cggttccagg cgaaattcca ttcggcgctg acgtagcgcg agccggccga
     1141 atccttcgac cacagcaaca ccacgtgccc gcaccgctcg atggcccgct gcagggcttc
     1201 gagcagcagc cccttgcggc gcaggtcctg gtggtccagc cacacggaga tgtcgttgtc
     1261 ggccagatgc tcgctgatcc gcgtggcgag cgcgtcgtcc tccgaggcgt ggctgatgaa
     1321 ggcgtcgcat gcgggtgccg cagacggatt ctgcactagc ctcccctggc cggaaggtac
     1381 tgcagcggat cgaccggctt gcccaggcgg cggatctcga agtgcagtcc cgggcgctcg
     1441 gcgtcgaagc tgcccatttc ggcgatcgcc tgtcccgccg ccacgcgctc cccttccttg
     1501 acgcgcagct cgcgattgtg cgcgtacacg ctcagataag tcgcgtcgtg cttgatgatc
     1561 accagcttgc cgtacccgcg cagcccgctg ccgctgtaca ccacccggcc ggcggcgctg
     1621 gccagcaccg gttgtcccgt ttctccgccg atcgcgatgc ccttgggatt ggggccctgg
     1681 ttgaatggct gcagcagcgc accgcgcgcc ggccaggccc aggtgatggc gcccgccgag
     1741 gcggtggccg acacgctcgc cggcgccgcc ggcggtgtca cgggcttggc gcgcgcagtg
     1801 ctgggagccg cctgcggcgg gccagcttgc gcactcggcg ggctcgccgg ccgcggcgcc
     1861 ggctccgatg cgggcttggt gggcgcccca cgcatctgcg ccaacgcctg ttcggaatag
     1921 cgcaccttga gcgctttggg cgccttcttt accaccgggg cttccagcgg cttggcctgg
     1981 atctcgggcg gcgcgtcgac ggcgcgcgcg accacggcgc cctcctcctc ggcgggatct
     2041 ttccagcccg ggggcgggcg cacgcgcagc tgctggtcta cccggatcat gttcgcgtcg
     2101 gtgaggccgt tccacgcggc cacgtccttg tagtcctggc cgttgtccag cgcgatgctg
     2161 tacagcgtgt cgcccttcct caccgtgtag aactccggca gcgcctcggc ctcggcgctc
     2221 gcgcgcaccg ccggggccgc cgggcgggat gccgctgctg tcgtagcaga aggcaccgcc
     2281 gctgttgcag cagaaggcgc cgtcgctgtt gcagcagaag gcgccgtcgc tgtcgtagca
     2341 gaaggcaccg gtgttgccgt agcagaagga actggtgttg ccgtagcaga aggaactggc
     2401 gttgccgtgg cgggaggcgc cgccgccgcg ggagatgtcg ccgtcgccgg gtgaggaaca
     2461 gcagccgccg gccgggcggg cgtcgcggcc ggacgcggcg cagg
//
LOCUS       DASPGE010000271         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1007735, whole genome shotgun sequence.
ACCESSION   DASPGE010000271 DASPGE010000000
VERSION     DASPGE010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007735"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VD791_08720"
     CDS             <1..1005
                     /locus_tag="VD791_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXV08099.1"
                     /translation="GAMLQHRHILANVQQILTFTGDTVRDSREIAIAPLPFYHILALV
                     LNCMMFVRAGGLQVLIANPRDIPALVKTLARSRFTFFVGVNTLFNALAHNPEFQRLDF
                     SNLRMSGGGGAPVQLAVAKRWKEITGRVLSEGYGLTETSGAVSINRQDLDEHTGTTGL
                     PLPSTEVEIRDAEGRVLPIGETGQVYVRGPQVMAGYWQRADETAKVLGPDGFLATGDV
                     GLITPEGLLKIVDRVKDMILVSGFNVYPNEVEEAVALHPGVLEACAIGVPDEVTGEAV
                     KLFVVRKDPALTAEQIVAHCRQNITGYKVPKHVEFRDTLPKSPVGKILRRELRDAAKA
                     "
     gene            1002..2201
                     /locus_tag="VD791_08725"
     CDS             1002..2201
                     /locus_tag="VD791_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV08100.1"
                     /translation="MNRTTLGAWIAALAALPAAAGQPIRIGEISSYSTVPAFTVPYRN
                     GWQLAVEEVNAVGGVLGRKLEVISRDDAGKPDDATRHANELLASHKVDLLAGTFLSNV
                     GLAVSDIALRNKVLFVAAEPLSDAIVWEKGNRYTFRLRPSTYMQAAMLVEEAAKLPAK
                     RWATVAPNYEYGQSAVASFRALLKARRPDVEFVAEQWPALGKLEAGATAQALAAAKPD
                     AIFNVTFGADLTKFVREGNLRGLFEGRAVVSMLTGEPEYLDPLKDETPAGWIVTGYPW
                     EQIDNPAHDKFVEGYRRRYNDTPRLGSVVGYTTFMAIVEAIRKAGSTDTEKLIRSFRG
                     LTVPTVFGPITFRAIDQQSTLGAFVGRLELKNGRGTMVDWRYADGKDYMPADAAVKKL
                     RPAEAMR"
     gene            2204..>2409
                     /locus_tag="VD791_08730"
     CDS             2204..>2409
                     /locus_tag="VD791_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV08101.1"
                     /translation="MTTYLIQALSGLSHAATLFLVACGLSIIFGVTRIVNFAHGSLYM
                     LGAYMAYSFGQSLAGPHRGRPGVMG"
BASE COUNT          388 a          831 c          825 g          365 t
ORIGIN      
        1 ggcgcgatgc tgcagcatcg tcacatcctg gccaacgtgc agcagatcct caccttcacc
       61 ggcgacaccg tgcgcgacag ccgcgagatc gcgatcgcgc cgctgccctt ctatcacatc
      121 ctggcgctgg tgctgaactg catgatgttc gtgcgcgccg gcgggctgca ggtgctgatc
      181 gccaacccgc gcgacatccc ggccctggtc aagaccctgg cgcgctcgcg cttcacgttc
      241 ttcgtcggcg tgaacacgct gttcaatgcg ctggcgcaca accccgagtt ccagcggctc
      301 gatttcagca atctgcgcat gtccggcggc ggcggggcgc cggtgcagct cgccgtggcc
      361 aagcgctgga aggagatcac cggacgcgtg ctgtccgagg gctacggcct gaccgagact
      421 tccggcgcgg tgtccatcaa ccgccaggac ctcgacgagc acaccgggac taccggcctg
      481 ccgctgccga gcaccgaggt cgagatccgc gacgccgagg gccgcgtgct gccgatcggc
      541 gagaccggac aggtgtacgt gcgcggaccg caggtgatgg ccggctattg gcagcgcgcc
      601 gacgagacgg ccaaggtgct gggcccggac ggcttcctcg ccaccggcga cgtcggcctg
      661 atcacgccgg aggggctgct caagatcgtg gaccgcgtca aggacatgat cctggtgtcc
      721 ggcttcaacg tctatccgaa cgaggtggaa gaggcggtcg cgctgcaccc cggtgtgctc
      781 gaggcctgcg ccatcggcgt gccggacgag gtgaccgggg aggcggtgaa gctgttcgtg
      841 gtgcgcaagg atccggcgct caccgccgag cagatcgtcg cccactgccg ccagaacatc
      901 accggctaca aggtgcccaa gcacgtggag tttcgcgaca ccctgccgaa gagcccggtg
      961 ggcaagatcc tgcggcgcga gctgcgcgac gcggccaagg cgtgaaccgc acgacactcg
     1021 gcgcctggat cgcagcgctt gcggcgctgc cggcggccgc cgggcagccg atccgcatcg
     1081 gtgagatcag cagctactcg acggtgccgg cgttcaccgt gccctaccgc aacggctggc
     1141 agctcgcggt ggaggaggtg aacgcggtcg gcggggtgct gggccgcaag ctcgaggtaa
     1201 tctcgcgcga cgacgccggc aagccggacg atgccacgcg ccacgccaac gagctgctcg
     1261 ccagccacaa ggtcgatctg ctggcgggca cgttcctgtc caacgtcggc ctggcggtga
     1321 gcgacatcgc cctgcgcaac aaggtgttgt tcgtcgccgc cgagccgctg tccgacgcga
     1381 tcgtctggga gaagggcaac cgctacacct tccggctgcg ccccagtact tacatgcagg
     1441 ccgccatgct ggtggaggag gcggccaagc tgcccgcgaa gcgctgggcc acggtagccc
     1501 cgaactacga gtacgggcag tcggcggtgg cgagcttccg cgccctgctc aaggcgaggc
     1561 gtcccgacgt ggagttcgtc gccgagcagt ggccggcgct gggcaagctc gaggccggcg
     1621 cgaccgcgca ggcgctggcc gcggcgaagc ccgacgccat cttcaacgtc accttcggcg
     1681 ccgatctgac caagttcgtg cgcgagggca acctgcgcgg actgttcgag ggccgcgccg
     1741 tggtgagcat gctcaccggc gagcccgagt acctggatcc gctcaaggac gagacgccag
     1801 cgggctggat cgtcaccggc tatccctggg agcagatcga caatcccgcg cacgacaaat
     1861 tcgtcgaggg ctaccggcgc cgctacaacg acactcctcg cctgggctcg gtggtcggct
     1921 acaccacgtt catggcgatc gtcgaggcga tccgcaaggc cggctccacc gataccgaaa
     1981 agctgatcag gtccttccgc ggcctgacgg tgccgaccgt gttcggaccg atcaccttcc
     2041 gcgcgatcga ccagcagtcc accctcggtg cgttcgtcgg ccgcctggag ctgaagaacg
     2101 gtcgcggcac catggtcgac tggcgctacg ccgacggcaa ggactacatg ccggccgatg
     2161 ccgcggtgaa gaagctgcgg ccggcggagg ccatgcggta ggcgtgacca cctacctgat
     2221 ccaggcgctg tcgggtcttt cgcatgccgc caccctgttc ctggtggcct gcgggctgtc
     2281 gatcatcttc ggcgtgaccc gcatcgtcaa tttcgcccac ggctctctct acatgctcgg
     2341 cgcctacatg gcgtacagct tcggccagag cctcgcgggc ccccaccggg ggcgccccgg
     2401 ggtgatggg
//
LOCUS       DASPGE010000272         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_775227, whole genome shotgun sequence.
ACCESSION   DASPGE010000272 DASPGE010000000
VERSION     DASPGE010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775227"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1870
                     /locus_tag="VD791_08735"
     CDS             <1..1870
                     /locus_tag="VD791_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HXV08102.1"
                     /translation="ALSMRVDSELELIRSEAQALLAEFQRYASYANAQAAYGRILNSL
                     GIDLLPDAVERADVNTLAVAVAAEIKQAERDTFPLALAPAMRQVAPLRVQIENVKDSA
                     LKAAAEEAVIGALRGAGYTVNAADPAAWSLRMRFDLARVRAGVDRGVWDIRLERADGT
                     LAGRAEYSSSLASQFQPSTMGAFAEAATVSQLRALGGWLRDEPSRAVPVADASAPPGA
                     KPSAHDTAIRTAAAAWAQAWAAKDVERYLASYAPTFKPPGGQSRKAWEKQRRERISGP
                     RSIQVAIRGVEVHQQDGDRAVATFRQDYRADSYQDSVRKTLELVRDGDRWLIVEERVD
                     SMSAENGAAPAVEPARLADAAAAPASAPAAESTPAAAPVPDPAKEPAPATAPAASAAT
                     APAPAPVPEAVAATQSAPAASEAPVAAPRPTTEAAPATAPAPEPLAATQSTPAASEAP
                     AAAPRPATEAAPETASGPAPAPASRSSEGLGDLQVLSKLGRPLHAEIEIIPLRSADAK
                     NQTVVVRLASENAFRQAGMKFHPALNGAEMTIESRDGRPIVALTTRLPVNELVIDVLI
                     ELEWGAKRFVREYAILLDPPTHHPPSPAAMAPARPAARAVPALDPVRDTTIDVEGA"
     gene            1867..>2060
                     /locus_tag="VD791_08740"
     CDS             1867..>2060
                     /locus_tag="VD791_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HXV08103.1"
                     /translation="MMRAIVLLAAAGFAVAPVKAQDPGAIRVLLTPELETTLASPMLG
                     RVRNVNVSLGSGFDENKVLV"
BASE COUNT          307 a          745 c          718 g          290 t
ORIGIN      
        1 cgcgctgtcg atgcgcgtgg actccgagct ggaactgatc cgcagcgagg cgcaggcgct
       61 gctggcggag ttccagcgct acgcctcgta cgcgaacgca caggccgcct acgggcgcat
      121 cctcaactcg ctcggcatcg acctgctgcc cgacgcggtc gaacgcgccg acgtgaatac
      181 gctcgccgtg gccgtggctg ccgagatcaa gcaggccgag cgcgacacct tcccgctcgc
      241 gctcgctccg gcgatgcggc aggtggcgcc gctccgagtg cagatcgaga acgtcaagga
      301 ctcggcgctc aaggccgctg ccgaggaagc ggtgatcggc gcgctgcgcg gcgccggcta
      361 cacggtgaac gcggcggacc ccgccgcttg gtcgctgcgc atgcgcttcg atctggcccg
      421 cgtgcgggcg ggcgtcgacc gcggcgtttg ggacattcgc ctggaacgcg ccgatggcac
      481 gcttgcgggc cgcgcggagt attccagctc gctggcgtcg cagttccagc cgtccaccat
      541 gggtgccttc gccgaagccg ctaccgtgtc gcagttgcgc gcgctcggcg gctggctgcg
      601 cgacgagcca tcgcgcgcgg tcccggttgc cgacgcgagc gcgccgccgg gcgcgaagcc
      661 gtcggcccat gacacggcga tacgcacggc cgccgccgcg tgggcgcaag cctgggcggc
      721 aaaggacgtc gagcgctatc tcgcgtcgta cgcaccgacg ttcaagccgc cgggcgggca
      781 atcgcgcaag gcatgggaga agcagcgccg cgaacgcata tcggggccac gcagcattca
      841 agtggccatc cgcggcgtcg aggtgcatca gcaggacggt gaccgggccg tggcgacgtt
      901 tcgccaggac taccgtgcgg acagttacca ggacagcgtg cgcaagacgc tcgaactggt
      961 gcgcgacggc gatcgctggc tgatcgtgga agagcgggtg gattcgatgt cggcggaaaa
     1021 cggcgcagca ccggcggttg agcccgcccg cctggcggat gccgccgctg cgcctgcgtc
     1081 cgcgccggcc gccgagtcca ccccggcggc ggctcccgta ccggatcccg caaaggagcc
     1141 cgcaccggca accgctcctg cggcaagcgc cgccacggca ccggctcctg cacccgtccc
     1201 ggaggcggtc gccgccacgc aatccgctcc cgctgcctcg gaggcgcctg ttgcagcccc
     1261 gcggcccacc accgaggccg cccccgcaac ggcacccgcc ccggagcccc tcgccgctac
     1321 gcaatccacc cctgctgcct cggaggcgcc tgccgcggcc ccgcggcccg ccaccgaggc
     1381 cgcccccgaa acggcatccg gcccggcacc cgcaccagcg agccgcagtt ccgagggcct
     1441 cggcgatctt caggtcctgt cgaagctggg ccggccattg catgcggaga tcgagatcat
     1501 cccgctgcgc agtgccgatg cgaagaatca gactgttgtc gttcggctcg catccgagaa
     1561 cgcgttccgg caggcgggca tgaagttcca tcccgcgctg aacggcgcgg agatgacgat
     1621 cgagtcgcgc gacggcaggc cgatcgttgc gttgacgaca cgcctccctg tgaacgagct
     1681 ggtaatcgat gtgctgatcg agctcgagtg gggcgccaag cgttttgtgc gcgaatacgc
     1741 aatcctgctc gatccgccga cccatcaccc gccgtcgcct gcggcgatgg cgccggcgcg
     1801 gccggcagcg cgggcagtgc ccgcgctcga tccggtgcgc gatacgacca ttgacgtgga
     1861 gggcgcatga tgcgcgccat tgttctgctt gctgcggccg ggtttgccgt cgcgcccgtg
     1921 aaagcccagg accccggtgc aatccgtgtt ctgctgacgc ccgagctcga gaccacgctg
     1981 gcgagcccga tgctgggccg cgtgcgcaat gtcaacgtct cgctcggctc cggcttcgat
     2041 gagaacaagg tgctggtgga
//
LOCUS       DASPGE010000273         6590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1243193, whole genome shotgun sequence.
ACCESSION   DASPGE010000273 DASPGE010000000
VERSION     DASPGE010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243193"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VD791_08745"
     CDS             complement(<1..561)
                     /locus_tag="VD791_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV08104.1"
                     /translation="MRSLFLRALAGTFSLAAVLPAGAQPNEQFIPAAFYWVGPYAAGG
                     SGISAGMLDYLSLVNERDGGVGGVKLTWEKCETEYNNTRGVECYERLKSRHPALFHPL
                     STGITYSLIDRAAVDHIPLVTIGYGRTDASDGRVFPYVFPLITNYWSQNTAKIRFIGM
                     REGGMEKLKGKKIVNLYHDSAYGKETI"
     gene            complement(731..2110)
                     /locus_tag="VD791_08750"
     CDS             complement(731..2110)
                     /locus_tag="VD791_08750"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HXV08105.1"
                     /translation="MAAAGESWSGRLGFILATVGSAVGLGSIWKFPYEVGANGGSGFL
                     LFYVLGLLLVVVPLMFAEFAIGRRGRGDPATSIAAVAAAHGARRRWALAGLLGVGTGF
                     LILSFYSVIAGWTLSYAFETALSGLPAAEPQAVQARFDALLGSPWRIAAWHGVFMAVC
                     AMIVARGISGGIEKACLMLMPLLAVLLLALAVYSVATGDLSATLRFLFKLDPQRLDGG
                     VALEALGLGFFSIGVGLGLMITYAAYAGAEISLRQVAVVSVAVDTAISFLAGFAVFPV
                     VFAHGLDPASGAGLVFVTLPLAFADMPLGTVAAVAFFVLLFVAALASAISLLEIVVAM
                     LVRRRGWTRGRASLAASAACFAAGIATVLSFNLWSAWYPLAWLSNLGLPGFGHATVFD
                     LLDHLTSNVLLPLTGLLLALFAGWALPERLFAEELRLGARGGRLLRALLRYIVPPAIA
                     AIGLAAVVA"
     gene            complement(2123..3481)
                     /locus_tag="VD791_08755"
     CDS             complement(2123..3481)
                     /locus_tag="VD791_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HXV08106.1"
                     /translation="MTRHLLDWIVAAGEDGPYGDAALLRSMVRFEVALAQAQARVGAI
                     PQAAADAIAQHASGLALDPAPLARAGAHAGTLVVPFVRALVSHVATRDESAARWIHHG
                     ATSQDVMDTALALCTRKALAALERDLSRASRAAAALARRHATAPMLARTLLQPAGVTT
                     AGFKCAHWALSLARVRWRLLRTAQEALAVSLGGAAGNLAELGEHGPAVRAQMAQALEL
                     TDPGTTWHSHREPWIALAADTGLGCGAMAKIARDVALMAQAEVAEAREPQADGRGASS
                     AMPHKRNPALTLRILATTQPVPGMISGLLAGMVQEHERALGNWQAELTQFPQVFTHAL
                     AAAAALGEMLEGLHIDTARCRANIEALHGTIFSERLAGLLLPVLGKEAAQATVADLCR
                     GALDGGRHLRDVARERLSGDARLQPHLGGELDEVFDLERPVRLSAAQIEPLLAAVERP
                     VP"
     gene            complement(3483..3953)
                     /locus_tag="VD791_08760"
     CDS             complement(3483..3953)
                     /locus_tag="VD791_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HXV08107.1"
                     /translation="MSKLTPSQTIGPFFHEGMRWALTPASAGAGAVSLTGRISDRDGK
                     PVDDAVLEVWQPGFGAAPFQRVYTGEAGAFAFAMPQPGSGQPHANVTVFTRGLTNGLF
                     TRVYLHAGDDPAKVQLPPGVPRERAGTLIARRDANGYHWDIRLQGEGETVFFEI"
     gene            complement(3960..4655)
                     /gene="pcaH"
                     /locus_tag="VD791_08765"
     CDS             complement(3960..4655)
                     /gene="pcaH"
                     /locus_tag="VD791_08765"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HXV08108.1"
                     /translation="MPNRLRPIPVGVHPPFLYPDYRSTTERAPSRPLIALEQGEPELT
                     VAHFPRNILKPTDADLTRQSSAGPLGQRIVVAGRVIDEDGRPARNSLVEVWQCNAAGR
                     YHHAKDQHDAPLDPHFHGYGKMLTDEHGNYRFVSIKPGAYPWRNHPNAWRPAHIHFSL
                     FGNAYAQRLITQMYFEGDPLLPLDPIYLSAPEGARRRLVSAFDLSLTLEGIALGYRFD
                     IVLRGRDATPMGV"
     gene            complement(4702..5742)
                     /locus_tag="VD791_08770"
     CDS             complement(4702..5742)
                     /locus_tag="VD791_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXV08109.1"
                     /translation="MSERSIPQLALTASLALGLCGPAAAQQIVLKLHHMLPPTSNPQQ
                     RLIQPWCDKINKESNDRLKCQIYAAMQLGGTPPQLFDQVRDGVADIVWTVPTYSAGRF
                     SKSEVFELPFMAHTAKAGSQALWQYVQKNSLDEFKGTHPILMHVHDGSLFHFTSKPVK
                     SLEDLRGMKIRAATRINARMLAALGATPVQMPLPQVPEALSKGVIDGAMVPWEGVPAI
                     KLHEIVKYHLETPPAAPRFSNTIFVMAMNPAKYDSLPPDLKKVIDNNSGLAMSAWAGE
                     NGWDAVVEPFRKLARDRGNSFTVLSDQELARWVKASENVDDTWVKEVTRKGADGRRLL
                     DEARALIKQYEK"
     gene            complement(5837..>6590)
                     /locus_tag="VD791_08775"
     CDS             complement(5837..>6590)
                     /locus_tag="VD791_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXV08110.1"
                     /translation="YILAIAIYVRLYPQHAPETQAASREQFLRGAIGVWPILAIFVVV
                     FGGIYGGFFTPTEAASIGAAMTFLTALLRGGLGGGRFAAALLSTAQTSGMIFMIFIGA
                     DMMNAALALSQLPAELASAVAHAGIPPLAIMAAIMLFYIALGCVMDELSMIMLTVPVI
                     FPVVLGLDFFGLPPAEKAIWFGILILMVVEIGMIFPPVGLNVYIMNGLAKDVPMAETY
                     KGVVPFLISDAIRMTLLILFPGITLWLARMLA"
BASE COUNT         1043 a         2252 c         2359 g          936 t
ORIGIN      
        1 gatggtttcc ttgccgtagg ccgagtcgtg atacaggttc acgatcttct tgcccttgag
       61 cttctccatc ccgccttcgc gcatgccgat gaagcggatc ttggcggtgt tctgcgacca
      121 gtagttggtg atcagcggaa acacgtaggg aaacacgcgc ccatccgagg cgtcggtgcg
      181 tccgtagccg atggtcacca gcgggatgtg gtccaccgcg gcgcggtcga tcagcgaata
      241 ggtgatgccg gtggacagcg ggtggaacag cgcggggtgg cggctcttga ggcgctcgta
      301 gcattccacg ccgcgcgtgt tgttgtattc ggtctcgcac ttctcccagg tgagcttcac
      361 cccgcccacg ccgccgtcgc gttcgttcac caggctcagg tagtcgagca tgccggcgct
      421 gatgcccgag ccgccggcgg cgtacgggcc gacccagtag aaggccgcgg ggatgaactg
      481 ctcgttcggt tgggcgcccg cgggcaggac agccgcgagt gagaaggtgc ccgccagggc
      541 acgcaggaac aaggaccgca tcgatctcct ccgttcgtta tggggcagtg cagcaaggct
      601 gcccgggagt tgtgctgccc atcctacgcc gcgaggcggg cgcctgccag tgtgcgccct
      661 taaggcgcga ttcaaaaaac tgcatcccac ctctctcacg aggcgcggga agcgcgaggg
      721 cgtggtccga ctacgccacc acggcggcca aaccgatcgc ggcgatggcg ggcgggacga
      781 tataacgcag cagggcgcgc agcagccgac cgccgcgggc gcccaggcgc agctcctcgg
      841 cgaacaggcg ctcgggcagg gcccagccgg cgaacagcgc cagcagcagg ccggtcagcg
      901 gcagcagcac gttggacgtg agatggtcga gcaggtcgaa cacggtggcg tggccgaagc
      961 cgggcagccc gaggttggac aaccaggcga gcggatacca cgcagaccag aggttgaagg
     1021 acagcacagt ggcgatgccc gcggcgaagc aagcggcgct ggccgccagg ctggcccttc
     1081 cgcgcgtcca gccgcgccgt cgcaccagca tcgccaccac gatctccagc agcgagatgg
     1141 ccgaggcgag cgccgcgacg aacagcagca cgaagaaggc caccgcggcc acggtgccga
     1201 gcggcatgtc ggcgaaggcg agcggcaggg tgacgaacac cagcccggcc ccgctcgcgg
     1261 ggtcgagccc gtgggcgaac accaccggga acacggcgaa gccggcaagg aacgagatcg
     1321 cggtgtccac ggcgaccgag acgaccgcga cctgcctgag gctgatctcg gcgccggcat
     1381 aggccgcgta cgtgatcatc aggcccagcc ccacgccgat ggaaaagaag cccagcccca
     1441 gcgcctccag cgcgactccg ccgtcgagcc gttgcggatc gagtttgaac aggaagcgca
     1501 gggtcgccga cagatcgccg gtcgccacgc tgtacaccgc cagcgcgagc agcagcacgg
     1561 cgagcagcgg catcagcatc aggcaggctt tctcgatgcc gccggagatg ccgcgcgcca
     1621 cgatcatcgc gcacaccgcc atgaacaccc cgtgccaggc ggcgatgcgc cagggcgagc
     1681 cgagcagcgc gtcgaagcgc gcctgcaccg cctgcggctc ggcggccggc agtccggaca
     1741 gcgcggtctc gaaggcgtag gacagcgtcc agccggcgat caccgagtag aagctcagga
     1801 tcaggaagcc ggtgcccacg ccgagcaggc cggcgagcgc ccagcgccgt cgcgcgccgt
     1861 gcgcggcggc caccgcggcg atgctggtgg ccggatcgcc ccggccgcgc cggccgatgg
     1921 cgaactcggc gaacatcagc ggcaccacca ccagcaacag gccgagcacg tagaacagca
     1981 ggaagccgct gccgccgttg gcgccgacct cgtaggggaa cttccagatg ctgcccaggc
     2041 ccaccgccga gccgacggtg gcgaggatga agccgaggcg cccggaccag ctctcccctg
     2101 cagcggccat cgagcgacgg ctctagggca ccggccgctc gacggcggcg agcaggggct
     2161 cgatctgcgc cgccgagagg cgcaccggcc gctcgaggtc gaacacctcg tccagctcgc
     2221 cgccgagatg aggctgcagg cgcgcgtcgc ccgacagccg ctcgcgcgcc acgtcgcgca
     2281 gatggcggcc gccgtccaat gcgccccggc acaggtcggc caccgtggcc tgcgccgcct
     2341 ccttgccgag caccggtagc agcaggccgg ccaggcgctc cgagaagatc gtgccgtgca
     2401 gcgcctcgat gttggcgcgg cagcgcgcgg tgtcgatgtg caagccttcc agcatctccc
     2461 cgagagccgc ggccgcggcc agcgcatgcg tgaacacctg cggaaactgc gtgagctccg
     2521 cctgccagtt gccgagcgcg cgctcgtgtt cttgcaccat gccggccagc agaccggaga
     2581 tcatgcccgg tacgggctgc gtggtggcga gaatgcgcag cgtgagggcc gggttgcgct
     2641 tgtgcggcat ggcggaggat gcgccgcggc cgtcggcctg gggctcccgt gcctccgcga
     2701 cctcggcttg cgccatcaac gcgacgtcgc gcgcgatctt cgccatggcg ccgcagccga
     2761 gcccggtgtc ggcggcgagc gcgatccagg gctcgcgatg gctgtgccag gtggtgccgg
     2821 ggtcggtcag ctcgagagct tgcgccatct gcgcgcgcac ggcggggccg tgctcgccca
     2881 gctccgccag gttgccggct gcgccgccca gcgaaacggc gagcgcctcc tgcgccgtgc
     2941 gcagcagccg ccagcgcacg cgcgcgagcg acagcgccca gtgcgcgcac ttgaagccgg
     3001 ccgtggtgac gccggcgggc tgcagcagcg tacgcgcgag catcggcgcg gtggcatggc
     3061 ggcgcgccag ggccgcggcg gcgcggctcg cgcgcgacag gtcgcgctcg agcgcggcca
     3121 gcgccttcct ggtgcacagc gccagcgcgg tgtccatcac gtcctggctg gtggcgccgt
     3181 gatggatcca gcgcgcggcc gactcgtctc gcgtggcgac gtgcgagacc agggcgcgca
     3241 cgaaaggcac gaccagcgtg ccggcgtgcg cacccgcgcg cgccagcggt gccgggtcga
     3301 gcgcgaggcc ggaggcgtgc tgagcgatgg cgtcggccgc cgcctgcggg atcgcgccga
     3361 cccgggcctg cgcctgcgcc agcgcgacct cgaagcgcac catgctccgc agcagcgccg
     3421 catcgccgta cgggccgtcc tcgcccgcgg cgacgatcca gtcgagcaga tgccgggtca
     3481 cgctagatct cgaaaaacac cgtttcccct tcgccctgca gccggatgtc ccagtggtag
     3541 ccgttcgcgt cgcggcgcgc gatcagggtg ccggcgcgct cgcgcggcac gccgggcggc
     3601 agctgcacct tcgccggatc gtcgccggca tgcaggtaca cgcgcgtgaa cagcccgttc
     3661 gtcagcccgc gcgtgaacac ggtgacgttg gcgtgcggct ggccgctacc gggttgcggc
     3721 atggcgaacg cgaacgcgcc cgcctcaccg gtgtacacgc gctggaacgg cgcagcgccg
     3781 aagcccggtt gccacacctc gagcacggcg tcgtccaccg gcttgccgtc gcggtcggag
     3841 atgcggccgg tcaggctgac cgctccggcg cccgcactcg ccggcgtcag cgcccagcgc
     3901 atgccctcgt ggaagaacgg gccgatggtc tgggaaggcg tgagcttgct catggttctc
     3961 tacaccccca tcggcgtggc atcgcggccg cgcagcacaa tgtcgaagcg gtagccgagc
     4021 gcgatgccct cgagcgtgag actcaggtcg aaggctgaca ccagacggcg gcgggcgccc
     4081 tcgggcgcgc tcaagtagat cgggtccagc ggcagcagcg gatcgccctc gaagtacatc
     4141 tgcgtgatca ggcgctgcgc gtaggcgttg ccgaagagcg agaagtggat gtgcgcaggc
     4201 cgccacgcgt tggggtggtt gcgccaggga taggcgcccg gcttgatgct gacgaagcgg
     4261 tagttgccgt gctcgtcggt cagcatcttg ccgtagccgt ggaagtgcgg atcgagcggg
     4321 gcgtcgtgct ggtccttggc gtggtggtag cgcccggccg cgttgcactg ccacacctcc
     4381 accagcgagt tgcgcgccgg cctgccgtcc tcgtcgatca cgcggccggc gacgacgatg
     4441 cgctggccga gcggccccgc gctcgactgg cgggtgaggt cggcatcggt gggcttgagg
     4501 atgttgcgcg ggaagtgcgc gaccgtcagc tccggctcgc cctgctcgag cgcgatcaac
     4561 ggccgggacg gcgcccgctc ggtggtgctg cggtaatccg gatagagaaa aggcggatgc
     4621 acgcccacgg ggatcgggcg caggcgattc ggcatcgcgt gttctgcgga agcgggtcgg
     4681 gtttgtttca tgccggtggc ctcacttctc gtactgcttg atcagcgcgc gcgcctcgtc
     4741 cagcaggcgg cgaccgtccg cgcctttgcg ggtgacttcc ttgacccagg tgtcgtccac
     4801 gttctcgctc gccttgaccc agcgcgccag ctcctgatcg ctcagcacgg tgaagctgtt
     4861 gccgcggtcg cgcgcgagct tgcggaacgg ctcgaccacc gcgtcccagc cgttctcgcc
     4921 ggcccaggcc gacatcgcca gcccgctgtt gttgtcgatc accttcttca ggtccggcgg
     4981 caggctgtcg tacttcgccg ggttcatcgc catcacgaag atggtgttgg aaaagcgcgg
     5041 cgcggcgggc ggtgtttcca ggtggtactt cacgatttcg tggagcttga tcgcgggcac
     5101 gccctcccac ggcaccatcg cgccgtcgat cacccctttg gacagcgcct cgggcacctg
     5161 cggcagcggc atctgcaccg gtgtggcgcc gagcgcggcc agcatgcgcg cgttgatgcg
     5221 ggtggcggcg cggatcttca tgccgcgcag gtcctccagc gacttcaccg gcttgctggt
     5281 gaagtggaac aggctgccgt cgtgcacgtg catcaggatc gggtgcgtgc ccttgaactc
     5341 gtccagcgag ttcttctgca cgtactgcca cagggcctgg ctgccggcct tggcagtgtg
     5401 tgccatgaac ggcagctcga acacctccga tttggagaag cgcccggccg agtaggtggg
     5461 cacggtccac acgatgtccg ccacgccgtc gcgcacctgg tcgaacagct gcggcggcgt
     5521 gccgccgagc tgcatggcgg cgtagatctg gcacttgagg cggtcgttgg attccttgtt
     5581 gatcttgtcg caccacggct ggatcagccg ctgctgcgga ttcgaggtcg gcggcagcat
     5641 gtggtgcagc ttgagcacga tctgctgcgc ggcggcgggc ccgcacaggc cgagcgccag
     5701 gctcgcggtc agcgccagct gcgggatgga tctttcagac acggtttctc ctgtgtaaac
     5761 ctcaccccgc cgcgtgggag gcggggaatg gcttcttaaa tcgcgcctta agggcgcact
     5821 cggcgcactc ggcgcactag gccagcatcc gcgccagcca cagcgtgatg ccggggaaca
     5881 ggatcagcag ggtcatgcgg atcgcgtccg agatcaggaa gggtacaacg cccttgtagg
     5941 tttccgccat aggcacgtcc ttggcgagcc cgttcatgat gtacacgttc aggcccaccg
     6001 gcgggaagat catgccgatc tccaccacca taaggatcaa gatgccgaac cagatcgcct
     6061 tctccgccgg cggcaggccg aagaagtcca gccccagcac caccgggaag atcaccggca
     6121 cggtgagcat gatcatggac agctcgtcca tcacgcagcc gagcgcgatg tagaacagca
     6181 tgatcgccgc catgatcgcg agcggcggga tgccggcgtg cgccaccgcc gaggccagct
     6241 ccgcgggcag ctgcgacagc gcgagcgcgg cgttcatcat gtcggcgccg atgaagatca
     6301 tgaagatcat gccgctggtc tgcgcggtgg acagcagcgc ggcggcgaac cgtccgccgc
     6361 ccagcccgcc gcgcagcagg gcggtgagga aggtcatcgc cgcgccgatc gaggcggcct
     6421 cggtgggggt gaagaagccg ccgtagatgc cgccgaacac caccacgaag atcgccagga
     6481 tcggccacac gccgatggcg ccgcgcagga actgctcgcg gctcgcggcc tgggtctcgg
     6541 gagcgtgctg cgggtacagc cgcacgtaga tggcgatggc gaggatgtag
//
LOCUS       DASPGE010000274         4427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1712513, whole genome shotgun sequence.
ACCESSION   DASPGE010000274 DASPGE010000000
VERSION     DASPGE010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712513"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /gene="glmU"
                     /locus_tag="VD791_08780"
     CDS             complement(<1..1108)
                     /gene="glmU"
                     /locus_tag="VD791_08780"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HXV08111.1"
                     /translation="MTSPSVVILAAGKGTRMLSSRPKVLHPIAGKPLLGHVLDTAQAL
                     SAPRVIVVYGYGGEAVPEAFAQSGAVFVLQEPQLGTGHALMQALPHLPADGTTLVLYG
                     DVPLTRKQTLERVLSHPGDLCLMTAMLDDPTGYGRILRDAAGAVTRIVEERDATADDK
                     RVREINTGIVAAPTAALRDWLSRLTKHNAQGEYYLTDIVPLALAGGIPVVTETVGEVW
                     EVQGVNSREQLAQLERRHQMEVARRLMAGGVTLADPARLDVRGELICAQDVSIDVDCV
                     FEGRVELASNVAIGPHCVIKDSDIAAGAVVAAFSHLDGASIGPGCRVGPYARLRRGAV
                     LAALARVGNFVEMKNTTLGEGSKANHLSYLGDTVV"
     gene            complement(1244..2683)
                     /gene="dacB"
                     /locus_tag="VD791_08785"
     CDS             complement(1244..2683)
                     /gene="dacB"
                     /locus_tag="VD791_08785"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HXV08112.1"
                     /translation="MPQSPYLVLLAAALALPGAASAANGRWPALLHAALAESGTPREA
                     VSLYVQEVGAREPLLAWNAGRAMNPASTMKLVTTLAALEVLGPTYTWRTEVYAQGPVL
                     GDVLHGDLVLKGYGDPKLTLENFWLLLRDLRARGLREIRGELVLDRSFFAPQNADPPR
                     FDSELMRPYNVPPDALLLNFKSVRLQFVPQEDSGTVKIVSTPHLPQIEIVNQLALGAG
                     HCDFWPERPQALPEQPRLVFTGVFPSGCGEKSRNFALLSPNDYALTLFQQLWRELGGA
                     FAGGVREGTVAPEATLLATWESPPLAEVIRDINKFSNNVMARQLFLTLSLAVEAPPLS
                     TGKAARAVREWARRVDLDLPELVIENGSGLSRIERISARGLARVLLRAMSAPLMPEYL
                     ASLPIPGIDGTLRRRLNASPASGQAHIKTGYLDGVRAIAGYVLDQQGRWLVVVAIINH
                     PSAINAQGFQDAVIEWAYSGEKPGRCCGP"
     gene            3009..3638
                     /locus_tag="VD791_08790"
     CDS             3009..3638
                     /locus_tag="VD791_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXV08113.1"
                     /translation="MELLGHIEEQMQSTRLPLFAITLAAVPYPDTPVILTLHWHGFVR
                     EKLAEVEGAQPVSYRSVPSSALQLNSRWEDVLTVDRAALDAAWELGAWDVARAERPSC
                     ARPGADSSEALDCLKAFGVFPFGAQADQVLVADAPDADELVSVAARRGYLMWLFRPVR
                     GGIWKEFADDATLLPDGTREPPCPLAPVPPRGDRAHRTVYCFGVSERPA"
     gene            3814..4101
                     /gene="groES"
                     /locus_tag="VD791_08795"
     CDS             3814..4101
                     /gene="groES"
                     /locus_tag="VD791_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HXV08114.1"
                     /translation="MRIRPLHDRVIVKRLEEERKTASGIVIPDTAAEKPDTGEVIAVG
                     TGKLLEDGKVRPLDVKAGDKILFGKYSGQTVKIEGQEYLVVREEDIMGVIE"
     gene            4155..>4427
                     /locus_tag="VD791_08800"
     CDS             4155..>4427
                     /locus_tag="VD791_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HXV08115.1"
                     /translation="MAAKEVKFHDSARHRIIAGVNVLADAVKVTLGPKGRNVVLERSF
                     GAPTVTKDGVSVAKEIELKDKFENMGAQMVKEVASKTSDVAGDGTTT"
BASE COUNT          726 a         1500 c         1530 g          671 t
ORIGIN      
        1 ccactacggt gtcgcccagg tagctcaggt ggttggcttt ggagccttcg cccaacgtcg
       61 tgttcttcat ctccacgaag ttgccgacgc gcgccagcgc cgccagaacc gcgccgcgcc
      121 gcaggcgcgc gtagggcccg acacggcaac ccggtccgat gctggcgccg tcaaggtggc
      181 tgaaggcggc cacgaccgcg ccggcggcga tgtcgctgtc cttgatcacg caatgcgggc
      241 cgatcgccac gttgctcgcc agctcaaccc ggccttcgaa gacacagtcc acgtcgatgc
      301 tcacatcctg cgcgcagatc agctcaccgc gcacgtccag gcgcgccgga tccgcgagcg
      361 tcacgccgcc ggccatcagc cggcgtgcga cttccatctg gtggcgccgc tcgagctgtg
      421 ccagctgctc gcggctgttg acgccttgca cttcccacac ctcccccacg gtctcggtga
      481 ccacggggat gccaccggcc agcgccagcg gcacgatgtc ggtcaggtag tactcgccct
      541 gcgcgttgtg cttggtcagg cgcgacagcc agtcgcgcag cgcggcagtc ggggcggcga
      601 cgatcccggt gttgatctcg cgcacccgct tgtcgtcggc ggttgcgtcc ctctcctcca
      661 cgatgcgggt caccgcgccg gcggcatcgc gcaggatgcg tccgtaaccc gtggggtcgt
      721 ccagcatcgc ggtcatcagg cacaggtccc cgggatgaga cagcacccgc tccagcgtct
      781 gcttgcgcgt cagcggcacg tcgccgtaga gcaccagggt ggtgccgtcg gcgggcagat
      841 gcgggagcgc ctgcatcagc gcgtgaccgg tgcccagctg tggctcctgc aacacgaaga
      901 cggcgcccga ctgggcgaag gcctcgggaa cggcctcgcc gccgtagccg tacacgacga
      961 tgacgcgagg tgcggacagc gcttgcgccg tgtcgagcac gtgacccagc agcggcttgc
     1021 cggcaatggg atgcagcacc ttcggccgcg aggacagcat acgagtgccc ttgccggccg
     1081 cgaggatgac gacgctcgga gaggtcatgg agtcgggcgg cgaggggagt ccgccccgcc
     1141 ccgggtacat ggcatcaagg tcatcggatt atggcacagg gctcggcggc ggcgctccgg
     1201 aactgtcaat ttccggcggc gacggacgcg cgcaaggggc gtctcatggt ccgcagcagc
     1261 ggcccggctt ttccccgctg tacgcccatt cgatgactgc atcctggaag ccctgggcgt
     1321 tgatcgcgga cggatggttg atgatggcca ccaccaccag ccaacgtccc tgctggtcga
     1381 gcacgtagcc ggcgatcgcg cgcacaccgt ccagatagcc ggtcttgatg tgcgcctgcc
     1441 cggaggccgg cgaggcgttg aggcgccggc gcagcgtacc gtcgatgccg gggatgggca
     1501 gcgacgccag gtactccggc atcaacggtg cactcattgc gcgcagcagc acccgggcga
     1561 gcccgcgagc gctgatgcgc tcgatgcgcg acagcccgga gccgttctcg atcaccagtt
     1621 cgggcaggtc caggtccacg cgccgcgccc attcgcgcac cgcgcgcgcg gccttgccgg
     1681 tggacagcgg cggcgcctcg acggccagcg acagggtgag aaacagctgg cgcgccatca
     1741 cgttgttgct gaacttgttg atgtcgcgaa tcacctccgc gagcggcggc gattcccagg
     1801 ttgcgagcag cgtggcctcg ggcgcgacgg tgccctcgcg cacgccgccg gcgaacgctc
     1861 cgcccagctc gcgccacagc tgctggaaca gggtcagtgc gtaatcgttc gggctgagca
     1921 gcgcgaagtt gcgcgacttc tcgccgcagc cactggggaa cacgccggtg aacaccagcc
     1981 gcggctgctc gggcagcgct tgcggtcgct ccggccagaa gtcgcagtgg ccggccccca
     2041 gggcgagctg attgacgatc tcgatctgcg gcaggtgcgg cgtggacacg atcttgaccg
     2101 tgccgctgtc ctcctgcgga acgaactgca gccgcaccga cttgaagttg agcagcagcg
     2161 catccggcgg cacgttgtag ggccgcatca gctcgctgtc gaagcgcgga ggatccgcgt
     2221 tctgcggcgc gaagaagctg cggtccagca ccagctcacc gcggatctcg cgcaggccgc
     2281 gcgcgcgcag atcgcgcaac agcagccaga agttttccag agtgagcttg ggatcgccgt
     2341 agcccttcag caccagatcg ccgtgcagca cgtcgccgag gaccggtccc tgcgcgtaga
     2401 cctcggtgcg ccaggtgtag gtgggcccca gcacttccag cgcggcgagc gtggtgacca
     2461 gcttcatggt cgaggcggga ttcatcgccc gccccgcgtt ccacgcgaga agcggctcgc
     2521 gcgcgcccac ctcctgcacg tacagtgaca ccgcctcgcg cggcgttccg ctttcagcca
     2581 gcgccgcatg cagcagcgcg ggccagcgcc cgttggccgc ggaagcggcg ccgggcagcg
     2641 cgagcgctgc ggcgagcaac acgaggtacg gcgattgcgg catgtcactg ctccagcgct
     2701 gcgctaagcg cgcgcagccg attgtaatgc aacggcacga cggccaatgt aacggcacgg
     2761 cggggctgcg ctgcaggtcg gattgtcgta gccttggcgg tgcgcgagac gctgctgcga
     2821 ggtcatggca gcgagcccgc gcgagtgcta acgcggtcgc cggcaccggg tatcgaccgg
     2881 aacttgcgcg gctgcgttgg caccaaatat gctggcggaa gggtatcggc gaggaacaat
     2941 cggcgtcggg cagcatcgcg ggccggtgcg cggcgcgcga gtcgataccg ccgaggagga
     3001 atctccgcat ggaactactg gggcacatcg aagagcagat gcagtcgacg cggttgccgt
     3061 tgttcgcaat cacgctggct gctgtgccat atcccgacac gccggtgatc ctgacgctgc
     3121 attggcacgg gttcgtgcgc gagaagctgg ccgaagtgga gggcgcgcag ccggtgtcct
     3181 accgctcggt gccaagctcg gcgctgcagc tgaacagccg ctgggaagat gtgctcaccg
     3241 tcgatcgcgc ggcgctggac gccgcctggg agctcggggc ctgggatgtg gcgcgcgccg
     3301 agcgcccgtc gtgcgcgcgc ccgggcgcgg actcgtcgga ggcgctggat tgcctgaagg
     3361 ccttcggcgt ctttcccttc ggcgcgcagg ccgatcaggt gcttgtcgcc gatgcgccgg
     3421 acgccgacga gctggtcagc gtcgccgcgc gccgcggcta cttgatgtgg ctgttccggc
     3481 ccgtgcgcgg cgggatctgg aaggagttcg cggacgacgc cacgctgttg cccgacggca
     3541 cgcgcgagcc gccctgcccg cttgcccccg tgcccccgcg cggggatcgc gcgcaccgca
     3601 cggtgtattg cttcggcgtc tccgaacgcc ccgcctgatg cgcccatagg gcgcgacgca
     3661 agcccccttt gcgcgcccgg atgggcgctc gggccgccca gtcacggctt gaaaatcgcc
     3721 ccgcccttgt ctatcattgg cactcactgc tagcgagtgc tagcagactc gcatttgcaa
     3781 tcgattcaac tacttggaga aggagatcgc acaatgagaa tccgccccct gcacgaccgg
     3841 gtgatcgtca agcggctgga agaggagcgc aagaccgcct ccggcatcgt catcccggac
     3901 accgcggccg agaagcccga caccggcgaa gtgatcgcgg tgggcaccgg caagctgctg
     3961 gaggacggca aagtgcgccc gctggacgtg aaagcgggtg acaagatcct gttcggcaag
     4021 tattccggtc aaaccgtcaa gatcgaaggc caggaatacc tggtcgtgcg tgaagaagac
     4081 atcatgggcg tgatcgagta agccgtcgcg ccgcgacaac acacccagcg aacacatact
     4141 caggagtcct gcacatggca gccaaagaag tcaagttcca cgattccgcc cggcatcgca
     4201 tcatcgcggg cgtgaacgtt ctggccgacg cggtcaaggt gaccctcggc cccaagggcc
     4261 gcaacgtggt gctggagcgc tccttcggcg cgcccaccgt caccaaggac ggcgtgtcgg
     4321 tggccaagga gatcgagctg aaggacaagt tcgagaacat gggcgcgcag atggtcaagg
     4381 aagtcgcgtc caagacctcg gacgtggccg gcgacggcac caccacc
//
LOCUS       DASPGE010000275         4487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_775537, whole genome shotgun sequence.
ACCESSION   DASPGE010000275 DASPGE010000000
VERSION     DASPGE010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775537"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1907
                     /locus_tag="VD791_08805"
     CDS             <1..1907
                     /locus_tag="VD791_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXV08116.1"
                     /translation="NFIANRVGVFSILAAFHHTRGLGLGFDVVDALTGPLIGRPKSAT
                     YRTADVVGLDTLAHVVNTMKEALREDPWHRHFDLPDWYRGLLDQGALGQKTQRGVYRK
                     VGRDIQVLDLAGKDYRLSGGEADPGVVEILKTRDVAERYQKLRASQHPQARFLWAALR
                     DMLHYCAYHLAGIADNARDLDFAVRWGFGWNQGPFEIWQAAGWQRVAGWIQEDIAAGR
                     SMASAPLPAWVLEKQREGVHAPQGSYSAATGKLQPRSGLPVYRRQPFPDRLVGEGGGY
                     GETVFETDAVRMWHTGDDIAVVTFKSKMHAIGEDVLDGVMQAIEEAERAFRGLILWQT
                     EPPFSVGANLSGPSARPADAPKPSAFGRMMKQFRRQAESAVLKAARTLNVADQLMAGR
                     LAKVEGIVAQFQAATQAWKYAQVPTVAAVDGLALGGGCEFIMHSARAVATLESYIGLV
                     EAGVGLLPAGGGCKELALRAAAEAKGGDVFPFLRQYFQNVAMAEVAKSAEQAREMHYL
                     KACDRIVMNRAELLHIAKSEVTALAEAGYRPPLRARAIPVAGRSAIATIRAYMANMLE
                     GQYISEHDFLVGSKIAHVLCGGDIEAGSLVDEQWFLELERTHFMELLATEKTQARIEH
                     TLKTGKPLRN"
     gene            1923..3122
                     /locus_tag="VD791_08810"
     CDS             1923..3122
                     /locus_tag="VD791_08810"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXV08117.1"
                     /translation="MSKQVQDAYIVAAKRTPVGKAPRGMFRNVRPDDLLAHALRSALA
                     QVPSLDPAAVDDVIVGCAIPEAEQGMNVARVGLLLAGLPNSVAGATVNRFCASGLQAV
                     AFAADRVRLGEADVMIAGGTESMSMIPMGGNKISFSEHIFDRSENIAIGYGMGITAEK
                     VALQWKVSREAQDRFATESHRKALAGIDQGEFADEIAPYEIDEKLPDLTAHDVRHAKR
                     VASVDEGPRPGTTPEVLAKLKPAFAAQGSVTAGNSSQTSDGAGAVIIASDAALKRFGL
                     QPLARMLGFAVAGVPPQIMGIGPKVAIPKVLKQVGLKQDDLDWIELNEAFAAQSLAVI
                     QDLGLDPAKINPLGGAIALGHPLGATGAIRTATVVHGLRRRRQKYGMVTMCVGTGMGA
                     AGVFERT"
     gene            3209..3661
                     /locus_tag="VD791_08815"
     CDS             3209..3661
                     /locus_tag="VD791_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HXV08118.1"
                     /translation="MRTLITTALAALMACVYLSAVADTATPAGMWRTIDDKTRKERSL
                     IRIVENNGVYEGKVERILNPQPDDDPNNLCRKCEGERKDKPVIGMTILWGLKKDGDHW
                     SGGEILDPKNGKIYRARMKLVDGGRKLEVRGFIGISLLGRSQTWLREE"
     gene            3663..>4487
                     /locus_tag="VD791_08820"
     CDS             3663..>4487
                     /locus_tag="VD791_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXV08119.1"
                     /translation="MPNARLKFWGWGREDVVLGAEEIRHLEGAYAKRFGLSSYEATPT
                     PRAEDIALRAPRVAVPGALADVCSTDRYERLLHSYGKSFFDSARVFARDFSNPPDAIA
                     FPRGEADIVTVLDWCDAIDAVVIPYGGGSSVVGGVEPPRTDRPVITLDLRHLDRVLEI
                     DRTSQAARIQGGVYGPSLESQLKPSGLTLRHFPQSFEFSSLGGWIATRGGGHYATLTT
                     HIDEFVESLRVLTPAGALQSFRLPGSGAGPSPDRMFIGSEGILGIITEAWMRLQDRP"
BASE COUNT          742 a         1463 c         1642 g          640 t
ORIGIN      
        1 ccaacttcat cgccaaccgc gtgggcgtgt tctcgatcct ggcggcgttc caccacacgc
       61 gcggcctcgg gctgggcttc gacgtggtgg acgcgctcac cgggccgctg atcgggcgcc
      121 cgaagagcgc cacctatcgc accgcggacg tggtcgggct ggacacgctg gcgcacgtcg
      181 tgaacacgat gaaggaggcg ctgcgcgagg acccctggca ccgccatttc gacttgccgg
      241 attggtatcg cggcctgctc gatcaggggg cgctcggcca gaagacccag cgcggcgtgt
      301 accgcaaggt gggcagggac atccaggtgc tggatctcgc cggcaaggac taccgcctct
      361 ccggcggcga agccgatccg ggcgtggtcg agatcctcaa gacccgggac gtggcggagc
      421 gttaccagaa gctgcgcgcc agtcagcatc cgcaggcgcg cttcctgtgg gcggcgctgc
      481 gcgacatgct gcactactgc gcctatcacc tggcgggcat cgccgacaac gcgcgcgatc
      541 tcgacttcgc ggtgcgctgg ggcttcggct ggaaccaggg gcccttcgaa atctggcagg
      601 cggcgggatg gcagcgcgtg gccgggtgga tccaggaaga catcgccgcg ggcaggagca
      661 tggccagcgc tccgctgccc gcatgggtgc tggagaagca acgcgagggc gtgcacgcgc
      721 cgcagggctc gtacagcgcg gccaccggca agctgcaacc gcgctccggc ctgcccgtgt
      781 accgccgaca accgtttccg gatcggctgg tgggcgaggg cgggggatac ggcgagacgg
      841 tgttcgaaac cgacgcggtg cgcatgtggc acaccggcga cgacatcgcg gtggtcacct
      901 tcaagagcaa gatgcacgcg atcggcgaag acgtcctcga cggcgtcatg caggcgatcg
      961 aggaggccga gcgcgccttc cgcggcctga tcctctggca gaccgagccg cctttctcgg
     1021 tgggggcgaa cctgtcggga ccctcggcgc ggccggcgga tgcgcccaag ccttcggcgt
     1081 tcggcaggat gatgaagcag ttccggcgtc aggcggagtc ggcggtgctc aaggcggcgc
     1141 gcacgctgaa cgtggcggat cagctcatgg ccggccggct ggcgaaggtg gagggcatcg
     1201 tggcgcagtt ccaggccgcg acgcaggcgt ggaagtatgc gcaagtgccg acggtggcgg
     1261 cggtggacgg gctggcactg ggaggaggct gcgaattcat catgcactcg gcgcgcgcgg
     1321 tggccacgct cgagagctat atcgggctgg tggaggcggg ggtggggctg ctgcccgcgg
     1381 gcggcggctg caaggagctg gcgttgcgtg ccgcggccga ggccaagggc ggcgacgtgt
     1441 tcccgttcct gcggcagtac ttccagaacg tcgccatggc cgaggtcgcg aagtcggcgg
     1501 agcaggcgcg cgagatgcac tacctcaagg cctgcgaccg catcgtcatg aaccgggccg
     1561 agctgctgca catcgcgaag tcggaggtga ccgcgctggc cgaggccggc tatcgcccgc
     1621 cgctgcgcgc gcgcgcgatc ccggtggccg gccgcagcgc catcgccacg atccgcgcct
     1681 acatggccaa catgctggaa ggacagtaca tctcggagca cgacttcctg gtcggcagca
     1741 agatcgcgca cgtgctgtgc ggcggcgaca tcgaggccgg cagcctggtg gacgaacagt
     1801 ggttcctgga gctggagcgc acgcacttca tggagctgct cgcgacggag aagactcaag
     1861 cgcggatcga gcatactttg aagaccggca agccgctgcg gaactgactc gagggcgaag
     1921 gcatgagcaa gcaagtgcag gatgcataca tcgtcgctgc gaagcgcacg ccggtgggca
     1981 aggcgccgcg cggcatgttc cgcaacgtgc gcccggacga tctgctggcg catgcgctgc
     2041 gctcggcgct cgcgcaggtg ccgtcgctcg atccggcggc ggtggacgac gtgatcgtcg
     2101 gctgtgcgat ccccgaggcg gagcagggca tgaacgtggc ccgggtgggc ctgctgctgg
     2161 cgggactgcc gaacagcgtg gccggcgcga cggtcaaccg cttctgcgcc tccggcctgc
     2221 aggcggtcgc gttcgcggcc gatcgcgtgc ggttgggcga ggccgacgtc atgatcgctg
     2281 gcggcaccga gagcatgagc atgatcccga tgggtggcaa caagatctcg ttcagcgagc
     2341 acatcttcga tcgcagcgag aacatcgcca tcggttacgg catggggatc accgcggaga
     2401 aggtggcgct gcagtggaag gtgtcgcgcg aggcgcagga tcggttcgcc accgagagcc
     2461 accgcaaggc cctggccggg atcgaccagg gcgagttcgc ggacgagatc gcgccctacg
     2521 agatcgacga gaagctgccc gatctcaccg cccacgacgt gcggcacgcg aagcgtgtgg
     2581 cgagcgtgga cgaggggccg cgccccggca ccacgccgga ggtgctggcc aagctcaagc
     2641 cggctttcgc cgcgcagggc agcgtcaccg ccggcaacag ctcgcagacc tctgacgggg
     2701 ccggcgccgt gatcatcgcc agcgacgcgg cgctcaagcg cttcggcctg cagccgctgg
     2761 cgcgcatgct cggcttcgcg gtggccggtg tgccgccgca gatcatgggc atcggcccga
     2821 aggtggcgat cccgaaggtg ctgaagcaag tcggtctgaa gcaggacgac ctggactgga
     2881 tcgagctaaa cgaagccttt gccgcgcaga gcctggcggt gatccaggat ctcgggctcg
     2941 accccgcgaa gatcaatccc ctgggcggcg cgatcgcgct cggccacccg ctgggcgcca
     3001 ccggggcgat ccgcaccgcc accgtggtgc acggcctgcg ccggcgcagg cagaagtacg
     3061 gcatggtcac catgtgcgtc ggcaccggca tgggcgccgc cggcgtgttc gaacgcacat
     3121 aggggccgcg tagcccggat gcagcgcagc ggaatccggg agaacggagg gccttgctca
     3181 caaagacaaa cgcagggagg agattgccat gcgtactctc atcaccaccg ccctggccgc
     3241 gctgatggcc tgtgtctacc tgtccgccgt ggccgacacc gccacgccgg cgggaatgtg
     3301 gcgcaccatc gacgacaaga cgcgcaagga gcgctcgctg atccgcatcg tcgagaacaa
     3361 cggcgtgtac gaaggcaagg tcgagaggat cctcaacccg cagcccgacg acgacccgaa
     3421 caatctgtgc cgcaagtgcg agggcgagcg caaggacaag ccggtcatcg gcatgaccat
     3481 cctgtggggg ctgaagaagg acggcgacca ctggagcggc ggcgagatcc tcgacccgaa
     3541 gaacggcaag atctaccgcg ccaggatgaa gctggtggac ggcggccgca agctcgaggt
     3601 gcgcggcttc atcggcatct cgctgctggg ccgctcgcag acctggctgc gcgaggagtg
     3661 acatgccgaa cgcaagactg aagttctggg ggtgggggcg cgaggacgtg gtgctcggcg
     3721 ccgaggagat ccggcacctg gaaggcgcct acgcgaagcg cttcgggctg tcgagctacg
     3781 aggcgacgcc gacgccgcgc gcggaggaca tcgcgctgcg cgcgccgcgc gtcgccgtgc
     3841 ccggcgcgct cgccgacgtc tgctccaccg atcgctacga gcggctgctg cacagctacg
     3901 gcaaatcgtt cttcgacagc gcgcgcgtgt tcgcgcgcga cttctccaat ccgccggatg
     3961 cgatcgcttt cccgcgcggc gaggccgaca tcgtcaccgt gctcgactgg tgcgacgcca
     4021 tcgacgcggt ggtgattccc tacgggggcg ggtcgagcgt ggtgggcggc gtcgagcccc
     4081 cgcgcaccga tcgcccggtg atcacgctcg atctcaggca tctcgatcgc gtgctggaga
     4141 tcgatcgcac ctcgcaggcg gcgcgcatcc agggcggggt ctacgggccg tcgctggaga
     4201 gccagctcaa gccgagcggc ctgacgctac gtcactttcc gcaatcgttc gagttctcca
     4261 gcctcggcgg ttggatcgcc acgcgcggcg gcggccacta cgccacgctc accacccaca
     4321 tcgacgagtt cgtcgagagt ctgcgcgtgt tgactccggc cggcgcgctg cagtccttcc
     4381 gcctgcccgg ctcgggcgcg ggccccagcc cggaccgcat gttcatcggc tcggaaggga
     4441 ttctcgggat cattaccgaa gcgtggatgc ggttgcagga ccgtccg
//
LOCUS       DASPGE010000276         8984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1595129, whole genome shotgun sequence.
ACCESSION   DASPGE010000276 DASPGE010000000
VERSION     DASPGE010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8984
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595129"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VD791_08825"
     CDS             <1..572
                     /locus_tag="VD791_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXV08120.1"
                     /translation="GFVVNYWFVCLLVPVLAGAGAVFLVRTNSKAAYYFDYMKLRLPL
                     LGPILQKIILARFANVFALMYQSGITILDAMKSSEDIVGNRVISDGLQRAGQQINAGE
                     SLSEAFQNLGLFPPLVIRMMRVGENTGALDSALLNINYFYERDVRESIDKALKLLEPA
                     LTLVLGGILALILFSVLTPIYDILGKIQF"
     gene            598..2118
                     /locus_tag="VD791_08830"
     CDS             598..2118
                     /locus_tag="VD791_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08121.1"
                     /translation="MAKKILLCASAFHLSAAVWSGRRLTVCRGFEDDEAGQRAFLNFL
                     HSARNVPVYLTADTVEEDYRFETLPHAFGRDRREMVERKLRQLYRSTPFYGASRQQRE
                     GDKRRDDRYLFAALTNGEVFNPWLQILQEVKAPIAGVFPLPVVMLALLKRLGADEPQL
                     LIVSRHSGGVRQTFVKDQTFRISRLTATRAGDQRANDAYAEEIRNTRMYLDALNVTHV
                     DDVVTVLILDPDGSLATLGDAVVADRRNLRAVRLGPDELAAKVGIDRASLEANQDALP
                     LYLLGLESPDFSLAPPALTAGYSRYRASRTIYALCAAVAVAGLAWTGLDMYRLMDLGQ
                     ARRDALAETQRQQRAYQEITRTFPPAPASAERLKQAVDVAARIGTMSPLPEKTYQVVS
                     QGLEANPALTLGGMTWRYGKPSGDGTAAAAQPVQSALLQVQVLAQPGDFTGALSAVNK
                     FVKDLARQEPVAAARTVKLPIDPGSSATLRGSTTDSRQEEPVKVQFEVEVVLKAGV"
     gene            2122..2742
                     /locus_tag="VD791_08835"
     CDS             2122..2742
                     /locus_tag="VD791_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08122.1"
                     /translation="MALSTRDIPALGRPLLALGLVVGLSAGSILLAERLVKEARGSLT
                     KAQADLGEARKRVQRSGEERDTILRYVGPYQTLAQRGIVGEEQRLAWVDALRTANHEA
                     QLYGVEYEVGAQQAYALAAEAGAGGLPVQQSVMKLKFGLLYEDDLLSFFRVLAAQDVG
                     AFTINQCALQRLVPEVVRATNTPTLRAECEVAWITIPAPAAAEGSS"
     gene            2739..3317
                     /locus_tag="VD791_08840"
     CDS             2739..3317
                     /locus_tag="VD791_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08123.1"
                     /translation="MMRRLALVALAAAACLPAASRAQAADGWYGRLFYTPAQRAALED
                     ARRRKVRTEELAVETAKRPPAPRSRKVTLTGIVQRSDGESFAWVNGKPVEGRTRDGLR
                     VRHSAGPGEVVVYDPQKGRTVSVKVGQRVDLLTGRIEEAYERNRAVPAQTVQDAAGSV
                     PAPAPEPPRNARSETEVEDEEQVDEDEADSKG"
     gene            3340..4626
                     /locus_tag="VD791_08845"
     CDS             3340..4626
                     /locus_tag="VD791_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08124.1"
                     /translation="MSTGCAGARQRGVALLALLVLIGVAGSLVLVSVASSSAQERDRH
                     EQSLRAMGLIRDALIARAAGDLNRPGSLPCPDYDGDGSADGSLCTSPYIGWVPWRTLD
                     LPDLRDAAGERFWYALSAEFRDHSSLGALNSETVGSLTADGNANPRYVAVVLAPNAPL
                     PSQNRDPSNTDAMAVRAQYLDGENADGDTEYATGMASDTFNDELLLITHEMLMPVVEQ
                     RVARQARKCLENFALQAGADGRFPFAAPLTDLTNFADAAATYYGRLPKTLDATNTALG
                     TTLAWPADDPQVPQSPGPEVTEYCLQAGTWWDYWSELLFYRVAPGYAPDVVTPGCGTC
                     LTVNNVGLTKAVVIVAGKAHAVSPAQARATALNKQLASNYLETAPSPPVSINNAFGLS
                     TGNLGRRPRAVSYSVATGNFNDRVECLNVGGNWPCD"
     gene            4617..5366
                     /locus_tag="VD791_08850"
     CDS             4617..5366
                     /locus_tag="VD791_08850"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXV08125.1"
                     /translation="MRLRRQTGRLSRHAGFTLVEMAVVLVVVALLLGSLLVPLSTQVE
                     QRQGAETELMLEDAREALIGFAMVNGRLPRPAQSSSNGAEKATGSCTGPSATINCTGF
                     LPWATLGLPRFDGYGKQLRYSVAPLYAESAITFSSSTAGHKTVKTRDGAGNPVNLVSG
                     VPAVILSHGANNFGFLESGSELGNGSATNADEAANDAKFKCTVAADCDNFMHRMRSNN
                     TGVTGGEFDDLVAWVPNTLLVNRLVAAGRLP"
     gene            complement(5332..7086)
                     /locus_tag="VD791_08855"
     CDS             complement(5332..7086)
                     /locus_tag="VD791_08855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV08126.1"
                     /translation="MSPASRPRLFSLRLFWRLRQQDWLALMLLGLQAALMFGITSPLS
                     KAFLLSHFGCFLLWQPVWRGEQKLYLGQGLIILGAAATLVASESWWLIALWICVLFAL
                     IGGEVPGIKNVGSRVVSLISATYLLSILLIWVVPHLFGSAQFSALFASAVRYAPLPLL
                     ALIFLIKTERAPRASAYSVDLVYSLLLFLMVMVLVLGAFVIRELSHGDYVLALAQALL
                     VTAGILVALSWLWDPRGGFAGIGQLMNRYFMSVGMPFERWMHNLANLADQERDPDKFV
                     KAAAQDIAALPWVTGIDWQTVAQRGMAGHVTRHATEVRLGALRLTLYTRWSPSPTLAL
                     HAHLLARLLADYYEAKQREQEQRRNAYMHAIYETGSRLTHDVKNLLQSLGSLCAAVET
                     SSEADAAALRRLLRRQLPQVTQRLQGTLDKLNRPPGAAPETGSALAWWRNLQQRYAHE
                     RVLFDAAGVPETAQLAVELFDSVAENLLQNAVAKRRSSPDLAIATTLAWNDGAALTVC
                     DSGAAIPEALAQQLFATPVASTEGLGVGLYQAARLATSSGYRLQLDSNVPGKVCFALR
                     RAAGPDALTAGDPRPPAG"
     gene            7201..8019
                     /locus_tag="VD791_08860"
     CDS             7201..8019
                     /locus_tag="VD791_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV08127.1"
                     /translation="MSKVLAIARFTLLEALRTRLPWLTLAVLGGLLLASLLVQQLAIT
                     ETLRLQMGFLAAGARLGAVFVLTLHVAGSMAREFNDKGVDLLLSLDLPRSGYYLGKLA
                     GYVGIAVAMAALTTAVLALPGLAAGALAWAGLALASAAPPAALPLWGVSLACELTLIA
                     TLTLFCVITFAQIMPAVSFVAAFYLLARSIAAMRLMSGSQLLAPEAWSTQVTGWLVDA
                     LALLLPDLSRFTGTAWLLDGAGGAAALAYVAAQTLVYGSLLAAAGLLDLYRKSL"
     gene            8065..8811
                     /locus_tag="VD791_08865"
     CDS             8065..8811
                     /locus_tag="VD791_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08128.1"
                     /translation="MAHVPLAVRVGAGVLLVAQALLSGWQPAPAATAQALPELPATPV
                     LRTASLGEPIPLAQLLMLYLQAFDNQPGISIPFVQLDYGRVEAWLTAALELDPAGQYP
                     LLMAAQVYGQVPDPARQRRMLDFVYRRFQADPDRRWRWLAHATIMARHRLHDPQLALQ
                     YARALRERATGPHVPGWARQMEIFLHEQAGEYAAARALLGGLLHSGAVTDAHELRFLT
                     ERLSQIESAENSSSASKRRLPHPAPPGAGR"
BASE COUNT         1376 a         3112 c         3145 g         1351 t
ORIGIN      
        1 cggggttcgt ggtcaactac tggttcgtct gcctgctggt cccggtgctg gccggcgccg
       61 gggccgtgtt cctggtgcgc accaattcga aggcggcgta ctacttcgac tacatgaagc
      121 tgcgtctgcc cctgctcggg ccgatcctgc aaaagatcat cctggcgcgc ttcgccaacg
      181 tcttcgcgct catgtaccag tccggcatca ccatcctgga cgcgatgaaa tcgagcgagg
      241 acatcgtcgg caaccgcgtg atctctgacg gcctgcagcg cgcggggcag cagatcaacg
      301 ccggcgagag cctgagcgag gcgttccaga acctgggctt gttccctccg ctggtgatcc
      361 gcatgatgcg cgtgggggag aacaccggcg cgctcgacag cgcattgttg aatatcaatt
      421 atttttacga acgcgacgtg cgcgagtcga tcgataaggc attgaagttg ctagagccgg
      481 ccctgacgct ggtgctcggc ggcatcctcg ccttgatatt gttttccgtg ctcacgccga
      541 tctacgatat cctgggcaag atccagtttt gagcgtcccc actgccggcg gtaagcgatg
      601 gcgaagaaga tcctactgtg cgcttcagcc ttccacctgt cggccgccgt ctggagcgga
      661 cgccggttga cggtgtgccg tggcttcgag gacgacgaag ccgggcagcg cgccttcctg
      721 aactttctgc actccgcccg caacgtcccg gtgtacctca ccgcggacac ggtcgaggag
      781 gactaccgct tcgagacgct gccgcatgcg ttcggccgcg atcggcgcga gatggtcgag
      841 cgcaaactgc gccagctgta tcgcagcacg ccgttctacg gcgccagccg gcagcaacgc
      901 gaaggcgaca agcgccgcga cgaccgctac ctgttcgcgg cgctgaccaa cggcgaggtg
      961 ttcaacccct ggctgcagat cctgcaggag gtcaaggcgc ccatcgccgg cgtgttcccg
     1021 ctgccggtgg tgatgctggc gctgctcaag cgcctcggcg cggacgagcc gcagctgctg
     1081 atcgtttcgc gccacagcgg cggggtgcgc cagaccttcg tcaaggacca gacgttccgc
     1141 atcagccgcc tgaccgcgac tcgcgcgggc gaccagcgcg caaacgacgc ctacgcggag
     1201 gagatccgca acacgcgcat gtatctggac gcgctcaacg tcacgcacgt ggacgacgtg
     1261 gtgacggtgc tgatcctcga cccggacggc tcgctggcca ccctcggcga tgcggtggtc
     1321 gcggaccgcc gcaacctgcg cgcggtgcgc ctggggcccg acgagctcgc ggccaaggtc
     1381 ggcatcgacc gcgcctcgtt ggaggcgaac caggacgcgc tgcctctgta cctgctcggg
     1441 ctcgagtcgc ccgatttcag cctcgcgccg cccgccctga ccgccggcta cagccgctac
     1501 cgggcctcgc gcaccatcta tgcgctgtgc gcggcggtcg ccgtggccgg cttggcctgg
     1561 accggcctgg acatgtaccg gttgatggat ctgggccagg cgcgacggga cgccctggcc
     1621 gagacccaga ggcagcagag ggcttatcag gagatcacgc gcaccttccc gccggctccg
     1681 gccagcgccg agcggctgaa gcaggccgtg gacgtggccg ctcgcatcgg caccatgtca
     1741 ccgttgcccg agaagaccta ccaggtggtc agtcagggcc tcgaggccaa tccggccttg
     1801 accctgggcg gcatgacatg gcgctatggc aagccgagcg gcgacggcac ggcggccgcc
     1861 gcacaaccgg tgcagtccgc cctgctgcag gtgcaggtgc tggcgcagcc gggtgacttc
     1921 accggcgcgc tcagcgcggt caacaagttc gtgaaggacc tggcgcggca agagccggtc
     1981 gccgccgcgc gcacggtgaa gctgcccatc gacccgggtt cgagcgcgac gctgcgcggc
     2041 agcaccacgg actcgcgcca ggaggagccg gtcaaggtgc agttcgaggt ggaagtggtg
     2101 ctcaaggcgg gggtgtagca cgtggccctt tcgaccagag acatccccgc tctcggccgg
     2161 ccgctgctgg cgctcgggct ggtcgtgggc ttgtcggccg gcagcatcct gctcgccgag
     2221 cgcctggtca aggaggcacg cggcagcctg accaaggcgc aggccgacct cggcgaagcg
     2281 cgcaaacgcg tgcagcgctc gggcgaggag cgcgacacca tcctgcgcta cgtcgggccg
     2341 tatcagacgc tcgcgcaacg cggaatcgtg ggcgaggagc agcgcctggc ctgggtcgac
     2401 gcgcttcgca ccgccaacca cgaggcgcaa ctgtacggcg tcgagtacga ggtgggcgcg
     2461 cagcaggcgt acgccctcgc tgcggaagcg ggtgcaggcg gcttgccggt gcagcaatcc
     2521 gtgatgaagc tcaaattcgg tctgctctac gaagacgatc tgctcagctt cttccgcgtg
     2581 ctcgccgccc aggacgtggg cgcgttcacg atcaaccagt gcgcgctcca gcgcctggtg
     2641 cccgaggtcg tgcgcgcgac caacacgccg acgctgcgcg ccgagtgcga ggtggcctgg
     2701 atcaccatcc cggcgcccgc ggcggcggag ggcagctcat gatgcgccgg ctcgctctgg
     2761 tggcgctggc cgctgccgct tgcctgcctg ccgcatcgcg cgcccaggcc gcggacggct
     2821 ggtacggccg gctgttctat acgcccgccc agcgcgccgc gctggaggat gcgcggcgcc
     2881 ggaaagtccg caccgaggaa ctcgccgtcg agaccgccaa gcgccccccg gcgccgcgct
     2941 cgcggaaggt gacgctcacc ggcatcgtgc aacgcagcga cggcgaatct ttcgcctggg
     3001 tgaacggcaa gccggtcgag gggcgcacgc gcgacggcct gcgcgtgcgc cactccgccg
     3061 gcccgggcga ggtggtggtc tacgacccgc agaaggggcg cacggtctcg gtcaaggtcg
     3121 gccagcgggt ggacctcctc accgggcgca tcgaggaagc ctacgagcgc aatcgcgccg
     3181 ttccggcgca gacggtgcag gacgcggcgg ggtcggtgcc cgcgcctgcg cccgagccgc
     3241 cgcgcaacgc gcgcagcgag acggaagtgg aagacgaaga gcaggtggac gaagatgaag
     3301 cggacagcaa gggttgatgt gcgcaccgga agcgttgatg tgagcaccgg gtgcgcgggg
     3361 gcacggcagc gcggcgtggc gctgctcgcc ctgctggtgt tgatcggggt ggcgggatcg
     3421 ctggtgctgg tgtcggtcgc cagcagctcg gcgcaggagc gcgaccgtca cgagcagtct
     3481 ctgcgcgcga tgggcctgat ccgcgatgcc ctgatcgcgc gcgcggcggg cgacctgaac
     3541 cgcccgggca gcctgccctg cccggattac gacggcgacg gcagcgccga cggcagcctg
     3601 tgcaccagcc cgtacatcgg ctgggtgccg tggcggactc tcgatctgcc cgatctgcgc
     3661 gacgccgccg gcgagcgctt ctggtacgcc ctgtcggccg aattccgcga ccactcttcg
     3721 ctgggtgcgc tcaactccga gacggtcggc tcgctgaccg cggacggcaa cgcgaatccg
     3781 aggtacgtgg ccgtcgtcct ggcgccgaac gcgccactgc ccagccagaa ccgcgacccg
     3841 tccaacaccg atgccatggc ggtgcgcgcg cagtacctgg acggcgagaa cgcggacggc
     3901 gacaccgaat acgccaccgg catggcgagc gacaccttca acgacgagct cctgctcatc
     3961 acgcacgaga tgctgatgcc ggtggtggag cagcgcgtgg cgcgccaggc gcgcaagtgc
     4021 ctggagaact tcgctctgca ggcgggtgcc gacgggcgct tcccgtttgc cgcgccgctc
     4081 accgatctga ccaacttcgc cgacgcggcc gccacgtact atgggcgcct gccgaagacg
     4141 ctggacgcca ccaacacggc gctgggcacc acgctcgcct ggccggccga cgatccgcag
     4201 gtgccgcaaa gccccggccc ggaagtcacc gagtactgcc tccaggccgg cacctggtgg
     4261 gattactgga gcgagctgct gttctatcgc gttgcgcccg gctacgctcc cgacgtcgtc
     4321 acgccgggct gcggcacgtg cctgacggtc aacaacgtgg gactgaccaa ggccgtggtg
     4381 atcgtcgccg ggaaggcgca cgcggtctcc ccagcccagg cgcgcgccac cgccttgaac
     4441 aagcagctcg cctccaacta cctggagacg gcaccctccc cgccggtgtc gatcaacaac
     4501 gccttcggcc tgtccacggg caacctcggc cggcgcccga gagcggtgtc ttacagcgtc
     4561 gccaccggca acttcaacga ccgcgtcgag tgcctcaacg tgggcgggaa ctggccatgc
     4621 gattgaggcg ccagaccggg cgattgagcc ggcatgccgg cttcacgctg gtcgagatgg
     4681 ccgtggtgct ggtggtagtg gcgctgctgc tcgggagtct gctggtcccg ctgtccacgc
     4741 aggtggagca gcgccagggc gccgaaaccg agctgatgct ggaggatgcg cgcgaggcgc
     4801 tgatcgggtt cgccatggtc aacgggcggt tgccgcggcc ggcgcagtcc tcgagcaacg
     4861 gcgccgagaa ggccaccggc agctgcaccg ggcccagcgc cacgatcaac tgcaccggtt
     4921 tcctgccctg ggcgacgctc ggcctgccgc gcttcgacgg ctacggcaag cagttgcgct
     4981 acagtgtcgc cccgctgtac gccgagtcgg cgatcacctt cagcagctcc accgccggtc
     5041 acaagacggt gaagacgcgc gacggcgccg gcaatccggt caacctggtg agcggcgtgc
     5101 cggccgtgat cctgtctcac ggcgccaata acttcggttt cctggagagc ggcagcgagc
     5161 tgggcaacgg ctccgcaacc aatgccgacg aggccgccaa cgacgccaag ttcaagtgca
     5221 cggtcgcggc cgactgcgac aacttcatgc accggatgcg ctcgaacaac acgggggtga
     5281 ccggcggaga gttcgacgac ctggtggcgt gggtccccaa cacgctgctc gtcaaccggc
     5341 tggtggccgc gggtcgcctg ccgtgagggc atccggcccg gccgctctgc gcagagcgaa
     5401 gcagactttg ccgggaacgt tgctgtcgag ctgcagacga tagccgctcg acgtggcgag
     5461 ccgggcggcc tggtacaggc ccacccccag cccctccgtc gaggccacgg gggtggcgaa
     5521 cagctgctgc gccagcgcct ccgggatcgc ggcgccgctg tcgcatacgg tcagcgccgc
     5581 accgtcgttc cacgccaggg tggtggcgat cgcgaggtcg gggctcgagc ggcgcttggc
     5641 gaccgcgttc tgcagcagat tctcggccac gctgtcgaac agctccaccg ccagttgcgc
     5701 cgtctcgggg acgcccgcgg cgtcgaacag gacgcgctcg tgcgcgtagc gctgctgcag
     5761 gttgcgccac caggcgagcg cggagcccgt ttccggcgcc gccccggggg gtcggttgag
     5821 cttgtcgagc gtgccctgca ggcgctgagt gacctgcggc agctgccgcc gcagcaggcg
     5881 ccgcagcgcg gccgcgtccg cctcgctgct cgtctccacg gccgcgcaca gcgaaccgag
     5941 cgactgcagc aggttcttga cgtcgtgggt gagcctggag ccggtttcgt agatcgcatg
     6001 catgtatgcg ttgcggcgct gctcctgctc gcgttgcttg gcttcgtagt aatcggcgag
     6061 caagcgggcc agcagatggg cgtgcaacgc cagggtgggg ctgggggacc agcgcgtgta
     6121 gagggtgagg cgcagggcgc ccaggcgcac ctcggtggcg tgacgcgtga catggccggc
     6181 catgccgcgc tgcgccacgg tctgccagtc gatgccggtc acccagggca gcgccgcgat
     6241 gtcctgcgcc gcggccttca cgaacttgtc cggatcgcgc tcctggtcgg ccaggttggc
     6301 caggttgtgc atccagcgtt cgaacggcat gccgacgctc atgaaatacc ggttcatgag
     6361 ctggccgatg ccggcgaagc cgccgcgcgg atcccacagc cagctcaggg caaccaggat
     6421 cccggcggtg accagcagcg cctgggccag ggccaggacg tagtcgccat ggctcagctc
     6481 gcggatgacg aaggcgccca gcaccagcac catcaccatc aggaacagca gcaggctgta
     6541 caccaggtcc acgctgtagg ccgaggcgcg cggagcgcgt tcggtcttga tcaggaagat
     6601 cagcgccagc agcggcaggg gcgcgtagcg cacggcgctc gcgaacaggg cgctaaactg
     6661 cgcgcttccg aacaggtgcg ggacgaccca gatcagcagg atggataaca gataggtggc
     6721 ggagatcagc gacaccacac ggctgccgac gttcttgatg cccggcacct cgccgccgat
     6781 caacgcgaac agcacgcaga tccacaacgc gatgagccac cagctttccg aggcgaccag
     6841 ggtggccgcc gcccccagga tgatcaggcc ctggccgagg tacagcttct gctcgccgcg
     6901 ccacaccggc tgccacagca ggaagcagcc gaagtgcgac agcaggaagg ccttggacag
     6961 cgggctcgtg atgccgaaca tcaaggccgc ctgcaggccc agcagcatga gggcgagcca
     7021 gtcctgctgg cgcaggcgcc agaacagacg cagggagaac aggcgcgggc gagaggccgg
     7081 gctcacgccg tcatcggagg cagccaaggt gcgtgaccga gcggtggacg ctgcatgcaa
     7141 tcgcgttgtg ggtgcaaacc ggaccgtcgt gtgtgccgac cggggcgttg tcttttggcc
     7201 atgagcaaag tcctcgccat cgcgcgtttc accctgctcg aagcgctgcg cacccggctc
     7261 ccctggctca cgctcgccgt cctgggcggc ctgctgctgg cgagcctgct ggtgcagcag
     7321 ctggcgatca ccgaaacgct gcggctgcag atgggcttcc tggcggcggg cgcgcgtctg
     7381 ggagcggtgt tcgtgctcac cctgcacgtg gccggcagca tggcgcgcga gttcaacgac
     7441 aagggcgtgg atctgctgct gtcgctcgac ctgccgcgtt cgggctatta cctgggaaag
     7501 ctcgccggct acgtcggcat cgcggtggcg atggccgcgc tgaccaccgc ggtcctggcg
     7561 ctgcccggcc tcgccgccgg cgccctggcg tgggccggtc tggcgctggc gtcggccgcg
     7621 ccgccggccg cgctgccgct gtggggcgtg tcgctcgcct gcgagctgac gctgatcgcc
     7681 actttgacgc tgttctgcgt catcaccttc gcgcagatca tgcccgccgt cagcttcgtc
     7741 gcggcattct acctgctcgc ccgcagcatc gccgcgatgc ggctgatgtc cgggtcgcag
     7801 ctgctggcac cggaggcctg gagcacgcag gtcaccgggt ggctggtgga cgccctggcg
     7861 ctgctgctcc cggacctgtc gcgcttcacc ggcaccgcct ggctgctgga cggcgcgggc
     7921 ggcgcggcgg cgctggccta tgtggcggcg cagaccctgg tctacgggtc cctgctcgca
     7981 gctgcgggcc tgctcgacct gtaccgcaag agcctctaga tgcgtccctt gtcatggctg
     8041 ccgggcgcgg cgcgagagcg accgatggcc cacgtcccat tggcggtgcg ggtgggcgcg
     8101 ggcgtcttgc tcgtggcgca agcgctgctg agcggttggc agccggcacc ggcggccacc
     8161 gcccaggcgc tgccggagct tcccgctacg ccggtgctgc gcacggccag cctgggcgag
     8221 ccgatccccc tggcgcaact gctcatgctc tatctgcagg ccttcgacaa ccagcccggg
     8281 atcagcatcc cgttcgtgca gctggactac gggcgtgtgg aggcgtggct gaccgccgcc
     8341 ctcgagctcg atcctgccgg ccagtacccg ctgctcatgg ccgcgcaggt ctacggtcag
     8401 gtccccgatc cggcccgcca gcgccgcatg ctcgacttcg tgtaccgccg cttccaggcc
     8461 gaccccgacc gccgctggcg ctggctggcg cacgccacga tcatggcccg gcaccgcctc
     8521 cacgaccccc agctcgctct gcagtacgcc cgggccctgc gcgagcgggc cacggggccg
     8581 cacgtgccgg gctgggcacg ccagatggag atcttcctgc acgagcaggc gggggagtac
     8641 gcggcggcac gcgcccttct gggtggactc ctgcacagcg gcgcggtcac cgacgcgcac
     8701 gagctgcgct ttctcactga gcgcttgtcg cagatcgaga gcgccgagaa ctcgtcatcg
     8761 gcgtcgaaaa ggcgacttcc gcacccggct ccgccgggcg ccggtcggta ggcttaaccc
     8821 tttgatctat cgcgcagtat cgtccggggg caagatcttc cgaggagcct gccgtcggcg
     8881 ggcgcgcgcg taacaatcgc tcctaagccc ctcaattttc gcttgaagtc gcggtggcac
     8941 ggttctggct gaggaccacc ccaggtgaag ccacaaccgg ccgt
//
LOCUS       DASPGE010000277         5561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1360523, whole genome shotgun sequence.
ACCESSION   DASPGE010000277 DASPGE010000000
VERSION     DASPGE010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360523"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="VD791_08870"
     CDS             complement(<1..1374)
                     /locus_tag="VD791_08870"
                     /inference="COORDINATES: protein motif:HMM:NF022518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HXV08129.1"
                     /translation="MREEALRTVLLVKAIEESDRAGTLLPLADRAAATRDAARAAGAA
                     AAPELDVEAAALPGKAQRLLAARAALLRRQLGTRHPFVETVLALSTGPGWIGRLLLVA
                     ALLLGFSLSALDGTRRIDMLSFPLLGLLLWNLLVYLLIVAGWVRVLFGQPRRRPAAGL
                     LARAALRPIGRLVARSAKFNAALAEALGRFLGEWSETARPLLLARMARLFHLSAAAVA
                     LGLIAGLYLRGMVLDYRAGWASTFLDAPQVHALLSVLYGPASSVLRMPIPDVSQLAAM
                     RWEGGRGGVGAAPWIHLIAGTALLFIVVPRLLLAALATLQTWRYALRAPLPPALVPYF
                     RSAFSTVEGVVGRGIVALMPYAYEPAPPAFAALRKLLPAALGENLAVDTHATVRYGEE
                     EAFLGHLAERGGAVADVIALLFNLAATPEDENHGAVIAGVRDWLTTSRHPAQLLLLVD
                     EGPYAERM"
     gene            complement(1374..2291)
                     /locus_tag="VD791_08875"
     CDS             complement(1374..2291)
                     /locus_tag="VD791_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXV08130.1"
                     /translation="MTYAPPKVVETSVFARLPERYKDAGEHNEWAAGQPLGMPYASLL
                     EGPSFDREGNLWCVDVPCGRVFKVSRDGAFQLVAEYDGWPNGLKFHRDGRIFIADYKH
                     GIMLLDPGSGAVTPLLVRAGPDRFKGVNDLFFAANGDLYFTDQGLTGLHDPSGRLFRM
                     RADGHVTCLLDNVPSPNGLVMNRDETALYLAVTRGNCVWRVPFVRSGAVAKVGVYLQL
                     SGGGGPDGLALDDQGRLAVAHVGMGSVWIFDRVGEPVYRVRSCMGAFTTNVAFGWPDR
                     NTLYITESESGTILKAELDAPGKPMYSHA"
     gene            complement(2302..3084)
                     /locus_tag="VD791_08880"
     CDS             complement(2302..3084)
                     /locus_tag="VD791_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXV08131.1"
                     /translation="MTDHLKFSVAEGIGRIVLDRPERMNAFTFEMIDAWHAALQRCRS
                     DDSVKVIIVTGTGNSFCSGGDIVEMGERLEQTPEQRKNELFQRIERIPLALEDLDKPV
                     IAAVNGVATGAGMDMALMCDIRYAAQSARFAETYIKVGLVPGAGGAHFLPRLVGVSKA
                     LELFFTGDFVDAQEALRLGIVNRVFPDATLMQEVEAIARRICEAPPLTLRMIKRAVYQ
                     GMRNDLRTNLDLISSHYAIITASQEHRDLVRRFIAAREKKRG"
     gene            complement(3081..4337)
                     /locus_tag="VD791_08885"
     CDS             complement(3081..4337)
                     /locus_tag="VD791_08885"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXV08132.1"
                     /translation="MRPEAKDGPLKNRPLQDGPLTGIRVLDLTSVVMGPFATHILADL
                     GADVIKVESPEGDSVRNYQPLRNPGMSGSFLNLHRNKRSIVLDLKRESSRAALDRLIG
                     GADVFVHSIRPRAIERLGYTYERVRALKPDIVYCGAYGFGAKGPYGDKAAYDDLIQAG
                     SGVAALYEEVHGEPGYVPTVICDKLTGQAVAYAILAALLQRERGGGGQAIEVPMLETT
                     VEFNLVEHMSGFAFEPPLGPPGFPRLMDRSRKPYRTRDGYACILPYSDRNWQDFYAFT
                     ARTEFRDDSRFARLPQRVRHIAVLYRMVEEEAAKRTTAEWVEFCDRASIPCMPVLGLE
                     QLPADPHLRAVGLFGVAEHPSEGKYRTVRSPIGFSNAPFRIRRHAPRLGEHTAEVLAE
                     IGMSDEEIARLTAPQPQQDRSDGAQP"
     gene            complement(4348..5448)
                     /locus_tag="VD791_08890"
     CDS             complement(4348..5448)
                     /locus_tag="VD791_08890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXV08133.1"
                     /translation="MGATRNGMAPQPDELSGIVLEQADRIFVGEVTAERLAAADRGEW
                     PHAIWAAVQDAGLTLALVPEAQGGVGLAAADGLRLMRRAGYHTVPLPLPETMVAAALW
                     AEAAGTAPEGILSLAPTAPDDVVRIERGPAASYVLHGAAQRVPWAQRASAIVMYARDS
                     AGSGYLALVPSGIARLTPCRNLAHEPRETLILEDVVLPAQAVHPAPASCAHGLLELGA
                     LIRAQQMVGAMERCLDHALRYAMERKQFGRPIGRFQAVQMMLAEAAGHYAAATAAADL
                     AAEAYGSAQLGFAVAVAKSRVGEAAGKVAEICHQVHGAMGFTQEHPLHFATRRLWSWR
                     DEFGHEPFWQARIGRLVCERGGEALWPLLTQS"
BASE COUNT          839 a         1978 c         1965 g          779 t
ORIGIN      
        1 catgcgctcg gcatacggcc cctcgtccac cagcagcagg agctgcgcgg ggtggcgcga
       61 ggtggtgagc cagtcgcgca cgccggcgat caccgcgccg tggttctcgt cctcgggcgt
      121 ggcggcgagg ttgaacagca acgcgatgac gtcggccacc gcgccgccgc gctccgccaa
      181 gtgcccgaga aaggcctcct cctcgccgta gcgcaccgtc gcatgggtgt cgaccgcgag
      241 gttctccccg agcgccgcgg gcagcagttt gcgcagcgcg gcaaaggccg gcggcgcggg
      301 ctcgtaggca tagggcatca aggcgacgat gccgcgtccc accacgccct ccacagtgct
      361 gaacgcggag cggaagtaag ggacgagcgc gggcggcagt ggcgcgcgca aggcgtagcg
      421 ccatgtctgc agggtagcga gcgcggccag cagcaggcgc ggaaccacga tgaacagcag
      481 cgccgtgccg gcgatcagat ggatccacgg cgcagcgccg accccgcctc gcccgccttc
      541 ccagcgcatc gccgcgagct gcgacacatc ggggatcggc atgcgcaaga cggaggatgc
      601 cggaccgtac agcaccgaga gcagggcatg cacctgcggc gcatcgagaa aggtgctggc
      661 ccaaccggcg cgatagtcca gcaccatgcc gcgcagatac agcccggcga tcaggcccag
      721 cgccaccgcg gccgcgctca ggtggaacaa ccgcgccatg cgcgccagca gcagcggacg
      781 cgcggtctcg gaccactcgc cgaggaagcg gccgagcgcc tcggcgagcg cggcgttgaa
      841 cttggcggag cgcgcgacca gccggccgat cggccgcagc gctgcgcgcg cgagcagccc
      901 cgccgccggc cggcgccgcg gttgtccgaa cagcacgcgc acccagccgg ccactatcag
      961 gagatacacc agcaggttcc acaagagcag cccgagcagc gggaaggaga gcatgtcgat
     1021 gcgccgcgtg ccgtcgagcg cggacagcga aaacccgagc agcagagccg cgaccagcag
     1081 cagccgtccg atccagcccg gcccggtcga cagagccagc accgtctcga cgaacggatg
     1141 gcgcgtgccg agctgacgtc gcagcaaggc cgcccgcgcg gcgagcaggc gctgcgcctt
     1201 gcccggcagc gccgcggctt cgacgtcgag ctcgggcgcc gccgcagcgc ccgcggcgcg
     1261 cgccgcatcg cgggtggccg cggcccggtc ggccagcggc agcagcgtgc ccgcgcggtc
     1321 gctctcctcg atcgccttca ccagcagcac ggtgcgcaac gcttcttcgc gcatcaggcg
     1381 tgcgagtaca tcggcttgcc gggcgcgtcc agctccgcct tgaggatggt cccgctctcg
     1441 gactcggtga tgtagagcgt gttgcggtcc ggccagccga acgcgacgtt ggtggtgaac
     1501 gctcccatgc aggagcgcac gcggtacacc ggctcgccca cccggtcgaa gatccacacg
     1561 ctgcccatgc cgacgtgcgc caccgccagc cggccctgat cgtcgagcgc cagcccgtcg
     1621 ggtccgccgc cgcccgagag ctgcaggtac acgccgacct tggccactgc gccgctgcgc
     1681 acgaacggca cgcgccacac gcagttgccg cgcgtgacgg caagataaag cgcggtctcg
     1741 tcgcggttca tcaccagccc gttcgggctc ggcacgttgt ccagcaggca ggtcacgtgg
     1801 ccatcggcgc gcatgcggaa caggcgcccg ctcgggtcgt ggaggccggt caggccctgg
     1861 tcggtgaagt acaggtcgcc gttggcggcg aagaacagat cgttgacgcc cttgaagcga
     1921 tccggcccgg cgcgcaccag caagggcgtc accgcgccgc tgcccgggtc gagcagcatg
     1981 atgccgtgct tgtagtcggc gatgaagatg cgcccgtcgc ggtggaactt caggccgttg
     2041 ggccagccgt cgtactcggc caccaactgg aaggcgccgt cgcgggagac cttgaatacg
     2101 cgcccgcagg ggacatcgac gcaccacaga ttgccctcgc ggtcgaagga gggcccctcg
     2161 agcagcgagg cgtacggcat gccgagcggc tggccggccg cccattcgtt gtgctcgccg
     2221 gcgtccttgt agcgctcggg caggcgcgcg aaaacgctgg tctcgaccac cttgggggga
     2281 gcgtacgtca ttggtccgac ctcagccgcg cttcttctcg cgcgcagcga tgaagcgccg
     2341 caccaggtcg cggtgttcct gcgacgcggt gatgatggcg tagtgcgagg agatcaagtc
     2401 gaggttggtg cgcagatcgt tgcgcatgcc ctggtacacg gcgcgcttga tcatgcgcaa
     2461 ggtgagcggc ggcgcctcgc agatgcggcg cgcgatcgcc tccacctcct gcatcagcgt
     2521 cgcgtcgggg aacacccggt tgacgatgcc caggcgcagc gcttcctgcg cgtcgacgaa
     2581 gtcgccggtg aagaacagct ccagcgcctt ggacacgccc accagccgcg gcaggaaatg
     2641 cgccccgccg gcgcccggca ccagcccgac cttgatgtag gtttcggcga agcgcgcgct
     2701 ctgcgccgcg tagcggatat cgcacatcag cgccatgtcc atgccggcgc cggtggccac
     2761 gccgttgacc gccgcgatca ccggcttgtc caggtcctcc agcgccagcg ggatgcgctc
     2821 gatgcgctgg aacagctcgt tcttgcgctg ctcgggcgtc tgttccaggc gttcgcccat
     2881 ctccacgatg tcgccgccgc tgcagaacga attgccggtg ccggtgacga tgatcacttt
     2941 gacgctgtcg tcgctgcggc agcgctgcaa cgccgcgtgc caggcgtcga tcatctcgaa
     3001 ggtgaaggcg ttcatgcgct cgggccgatc cagcacgatg cggccgatgc cctcggcgac
     3061 tgagaatttg aggtggtcgg tcatggttgt gctccgtcgg atcggtcttg ctgtggctgc
     3121 ggcgcggtga gccgggcgat ctcctcgtcg gacatgccga tctcggcgag cacctccgcc
     3181 gtgtgctcgc cgaggcgcgg tgcatgacgc cggatgcgga acggcgcgtt gctgaagccg
     3241 atggggctgc gcaccgtgcg atacttgcct tcgctcggat gctcggccac cccgaacagg
     3301 ccgaccgcgc gcaggtgcgg gtcggcggga agctgctcga gccccagcac cggcatgcag
     3361 gggatgctcg cgcggtcgca gaattccacc cattcggcgg tggtgcgctt ggcggcctct
     3421 tcctcgacca tgcggtagag cacggcgatg tggcgcacgc gctgcggcag gcgcgcgaag
     3481 cgcgagtcgt cccggaactc ggtgcgggcg gtgaacgcgt agaagtcctg ccagttgcgg
     3541 tccgagtacg gcaggatgca ggcgtagccg tcgcgcgtgc ggtaaggctt gcgcgagcgg
     3601 tccatcaggc gcggaaagcc gggcggaccg agcggcggct cgaaggcgaa gccggacatg
     3661 tgctctacca ggttgaactc caccgtggtc tcgagcatcg gcacctcgat ggcctgcccg
     3721 ccgccgccgc gctcgcgctg caacagcgcc gccagaatgg cgtaggcgac ggcttgtccg
     3781 gtgagcttgt cgcagatcac cgtgggtacg tagccgggct cgccgtgcac ttcctcgtac
     3841 agcgccgcca cccccgagcc ggcctggatc agatcgtcgt acgcggcctt gtcgccgtac
     3901 ggccccttgg cgccgaaccc gtaggcgccg caatagacga tgtcgggctt gagcgcgcgc
     3961 acgcgctcgt aggtgtagcc caggcgctcg atggcccgcg gccggatact atgcacgaac
     4021 acgtcggcgc cgccgatcag gcggtccagc gccgctcggc tcgactcgcg cttgaggtcg
     4081 agcacgatgc tgcgcttgtt ccggtgcagg ttgaggaagc tccctgacat gcccgggttg
     4141 cgcagcggct ggtagttgcg caccgagtcg ccttccgggc tttccacctt gatcacgtcg
     4201 gcgcccaggt ccgccaggat gtgcgtggcg aacgggccca tcaccacgct ggtgaggtcg
     4261 aggacgcgga tgccggtgag cggcccgtcc tgcagcggcc ggttcttcag cggtccgtct
     4321 tttgcctctg ggcgcatgcg ggccgactca gctctgggtc agcaagggcc agagcgcctc
     4381 gcccccgcgc tcgcacacca gccggccgat gcgcgcttgc cagaacggct cgtgcccgaa
     4441 ctcgtcgcgc caggaccaca ggcgccgcgt ggcgaagtgc agcggatgct cctgcgtgaa
     4501 gcccatggcg ccgtgcacct ggtggcagat ctcggcgacc ttgcccgcgg cctcgccgac
     4561 gcgcgacttg gccaccgcta cggcgaagcc gagctgcgcg ctgccgtacg cctccgccgc
     4621 gagatcggcg gcggcggtgg cggcggcgta atgcccggcg gcctccgcca gcatcatctg
     4681 caccgcctgg aatctgccga tcggccggcc gaactgcttg cgctccatgg cgtaacgcag
     4741 cgcatggtcc aggcaacgct ccatcgcgcc gaccatctgc tgcgcgcgga tcaaggcgcc
     4801 gagctcgagc agcccgtgcg cgcaggatgc gggcgccggg tgcaccgcct gtgcgggcag
     4861 cacgacatcc tccaggatca gcgtctcgcg cggctcgtgg gcgaggttgc ggcaaggcgt
     4921 cagcctggcg atccccgacg gtaccagcgc caggtagccc gaccctgcgg agtcgcgcgc
     4981 gtacatgaca atcgcggacg cgcgttgcgc ccagggcacg cgctgcgccg cgccgtgcag
     5041 gacatagctc gccgccggcc cgcgctcgat gcgcacgacg tcgtccggcg cggtgggcgc
     5101 gagcgacagg atgccctcgg gcgccgtgcc ggcggcctcg gcccacagcg ccgcggcgac
     5161 catggtttct ggcagcggca gcggcacggt gtgatagccg gcacggcgca tcaggcgcaa
     5221 tccgtcggcc gctgcgagcc cgacgccgcc ctgcgcttcc ggcaccagcg cgagcgtcag
     5281 ccccgcgtcc tgcaccgccg cccagatcgc gtgcggccac tcgccccggt cggcggcagc
     5341 gaggcgctcc gccgtcactt ctccgacgaa gatgcggtcg gcctgctcga gcacgatgcc
     5401 ggacagctcg tccggctgcg gcgccatgcc gttgcgtgtg gcgcccatca gcgcaacccc
     5461 agcccgcgcg cgatgatgcc gcgcaggatc tcgcgcgtgc cgccgcgcaa cgtgtaggcg
     5521 ggcgcgtaca gcaggaaatg tcccagcgtc tcggcgaaca c
//
LOCUS       DASPGE010000278        16811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1596351, whole genome shotgun sequence.
ACCESSION   DASPGE010000278 DASPGE010000000
VERSION     DASPGE010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16811
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596351"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VD791_08895"
     CDS             <1..224
                     /locus_tag="VD791_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08134.1"
                     /translation="LAAQYPRVLNRIAAVWYKRAEADRCFDELLLDARRTRQGFPQSV
                     VYEIASLRHHHVTHVFPKHVDPWEQSQLR"
     gene            complement(366..1475)
                     /gene="dnaJ"
                     /locus_tag="VD791_08900"
     CDS             complement(366..1475)
                     /gene="dnaJ"
                     /locus_tag="VD791_08900"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HXV08135.1"
                     /translation="MAKRDYYEVLGVNRDASEEDIKKAYRKLAMQHHPDRNPDNPKAE
                     DKFKEAKEAYEVLCDADKRAAYDRFGHRGVEAGVGMGAEAGGFADAFGDIFSEIFGGG
                     RQRSNVYRGADLRYNLEITLEDAARGTETKIRIPTLEACETCHGSGAKAGTEPVSCPT
                     CHGQGQVRIQQGFFSLQQTCPRCGGSGKFIREPCQTCGGAGRVKRQKTLSVKIPAGVD
                     EGDRIRLSGEGEAGVNGGPPGDLYVVIRLKAHAVFQREANDLHCEMPISFTAAALGGE
                     IEIPTLDGSAKIKIPPETQSGRVFRLRGKGIKGVRSHAHGDLLCHVVVETPVNLTARQ
                     RELLLELEAINQEESGRHDPRAKSWMERVKEFFQG"
     gene            complement(1712..3658)
                     /gene="dnaK"
                     /locus_tag="VD791_08905"
     CDS             complement(1712..3658)
                     /gene="dnaK"
                     /locus_tag="VD791_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HXV08136.1"
                     /translation="MAKIIGIDLGTTNSCVAIMENGQPKVIENSEGSRTTPSIVAYTE
                     EAEVLVGAPAKRQAVTNPKNTLYAVKRLIGRRFQDAEVQKAIKLVPYNISQAPNGDAW
                     VEARGKKMAPPEISAQVLMKMKKTAEDYLGETVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGLDKKQGDRKIAVYDLGGGTFDISIIEIAEVEGEHQFEV
                     LATNGDTFLGGEDFDQRLMDYIADEFRKDQGVDLRKDILALQRLKEAAEKAKIELSST
                     QQTEVNLPYITMDQSGPKHLAMKITRAKFESLVEDLIEKTARPCETAIKDSGVKVSEI
                     DDVILVGGMTRMPKVQDKVKEIFGKEPRKDVNPDEAVAMGAAIQGGVLKGEVKDVLLL
                     DVTPLSLGIETLGGVMTKLIQKNTTIPTKQTQVFSTADDNQAAVTIHVLQGEREMAGG
                     NKSLGQFNLEGIPPAPRGMPQIEVTFDIDANGILHVSAKDKATGKENKIKITASSGLS
                     EDEIKRMVKDAESHADEDRKARELVDARNQCDAMVHTVRKSLAEYGDKLDAAEKSRIE
                     DAIKAAEEAMKGNDHSTIQSKTQALAQAAQKLGEKMYAQQEAGAGAAGAGADASAGGA
                     GQGGKAQEGDVVDAEFEEVKDGKK"
     gene            complement(3770..4330)
                     /gene="grpE"
                     /locus_tag="VD791_08910"
     CDS             complement(3770..4330)
                     /gene="grpE"
                     /locus_tag="VD791_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HXV08137.1"
                     /translation="MQEQEPSLTSPAPGGAQETPAAAEPGPAAESETMPSQQEIIRQL
                     ELQAQEHYDAWLRAKAEADNIRKRAQADIANAHKYALETFAAELLPVRDALEAALAAD
                     NTTIDALRQGVELTLKQIDGAFEKAAIKEINPVGERFDPHRHQAMTTVESDKAANTVV
                     HVLQKGYLLHDRVLRPALVTVSKAPQ"
     gene            complement(4437..5486)
                     /gene="hemH"
                     /locus_tag="VD791_08915"
     CDS             complement(4437..5486)
                     /gene="hemH"
                     /locus_tag="VD791_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HXV08138.1"
                     /translation="MRYLPEPALPQGTLAKIGVLLINLGTPEAPSAAAVRPYLKQFLS
                     DPRVVEIPPALWWPILNGVILNLRPRQSADKYAKIWTAEGSPLKVHTRRQAQLLRERL
                     AARIDRPVAVEWAMRYGSPPVSQALASLREQGCDRLLVIPLYPQYAASTTASAYDAVC
                     AVLGRLRNAPALRLTKHFHDHPGYIAALAHSVREHWTAHGHPDLLVMSFHGLPRFAVD
                     RGDPYYGECRETARLLAEALGLRAEQHLTTFQSRFGRARWLEPYTIDTMQALGKKKLR
                     RVDVICPGFVADCLETLEEIAMENRAAFLAAGGGEFRTIPCLNERDDWIQALADLTMS
                     NLGGWLTPARAGAWI"
     gene            complement(5483..6514)
                     /gene="hrcA"
                     /locus_tag="VD791_08920"
     CDS             complement(5483..6514)
                     /gene="hrcA"
                     /locus_tag="VD791_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HXV08139.1"
                     /translation="MLNERAQILLKTLVERYIAEGQPVGSRTLSRYSGLDLSPASIRN
                     VMSDLEEMGLVASPHTSAGRVPTPRGYRFFVDSLLTIKPLDRVEIDQLEGQLHVERPH
                     RLLQSASHLLSDLTRFAGIVMAPRRSTQFRHVEFLRLSEKRVLLIIVTLEGDVQNRML
                     FTERDYTQSELIEAANFVNQAYQGLTFEQVKARLRDELERLRDDMTQLLTKAVEAGNE
                     ALTEQPEEYVISGEHNLLHVQDLSSNVLSLRRLFDLFEQRTGLLQLLEMGSRAHGVQI
                     YIGGESGLVPLDECSVVTAPYEVDGEVLGTVGVIGPTRMAYERVIPIVEITAKLLSSA
                     LAQRARALE"
     gene            6611..7483
                     /locus_tag="VD791_08925"
     CDS             6611..7483
                     /locus_tag="VD791_08925"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003273440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HXV08140.1"
                     /translation="MPPQFKTIALIGKYKSPEIAQPLLELARFLEARGVRVLLDPLTA
                     AHVARNNLEVLPLEQSGPLADLAVVIGGDGTMLNIARTLVPFDVALVGVNQGRLGFLT
                     DISTDNMLATIGAMLDGEFIVESRMLLQSEVRRGPQAIFDVLAFNDASVAKGADGGLI
                     ELEVKIDEQFVYVLRADGLVVSTPTGSTAYALSAGGPILHPALSAFAIVPVCPHTLSN
                     RPIAVGSDARIEVLMHRCADARVHYDSHSHCELQPGDRILVRRYPRSIRLLHPVGHNY
                     YHMLREKLHWSETL"
     gene            7502..9175
                     /gene="recN"
                     /locus_tag="VD791_08930"
     CDS             7502..9175
                     /gene="recN"
                     /locus_tag="VD791_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HXV08141.1"
                     /translation="MLRSLSIRDFVIVDRLDLEFQDGFTVLTGETGAGKSILVDALAL
                     VLGSRSDAGQVREGAARAEICAEFALDALPQAQAWLQTNDLADEPGLCLLRRVLEAGG
                     RSRAYVNGRPATLQQLEELAEMLVDIHGQHEHQSLLRPAAQRELLDAYAGASALARDV
                     AGAWREWQEVRRQRLEWEKNADALAAERERLQWQRQELERLAFAADEWGELQLDHKRL
                     AHAASLVEAVESALEALSEGEAAALAAVNAAVSRLNAAAEYDPGLKETLDVLEPAQIQ
                     LQEAVYGLRHYRNRIDLDPRRLQEVEQRLEALHTTARKYRVAPPQLPELLADAARRLE
                     EIGAGASPEALAKREREAQARYRTVAEKLGAERARTAQELGRRVTEQMQGLAMAGGRF
                     EAVLKPLAEGSAHGMEQIEFLVAANPGATARSLAKVASGGELSRISLAIQTVTSTVAP
                     VPTLVFDEVDAGIGGRVAEIVGRMLAALGSKHQVMCITHLPQVAAAAHHQWQVSKTAA
                     GNGVRSEVRELDRAQRVEEIARMLGGVKITETTRKHAAEMLGERRDRKA"
     gene            complement(9282..9719)
                     /gene="fur"
                     /locus_tag="VD791_08935"
     CDS             complement(9282..9719)
                     /gene="fur"
                     /locus_tag="VD791_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HXV08142.1"
                     /translation="MTKPHDLKTIGLKATLPRLRILNLFETAEVRHMTAEDVYKLLLK
                     EGMDIGLATIYRVLTQFEQAGILIRHHFEAGKAVFELNQGGHHDHLVCVQCGRVEEFY
                     DAEIERRQAKIAKERGFNIHEHSLQIYADCTKANCPYRTHGKA"
     gene            9803..10162
                     /locus_tag="VD791_08940"
     CDS             9803..10162
                     /locus_tag="VD791_08940"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HXV08143.1"
                     /translation="MLFRLLLLAACLALSSCFLKPHKIDVQQGNYLDEEMIAKLKPGM
                     TRSQVRFLLGTPLIADPFHPERWDYLLLDRREGKLVRDRRLTVWFEGDKLIRAVTDQP
                     DAPAKQRQPEQSAQQTR"
     gene            10166..10975
                     /gene="dapB"
                     /locus_tag="VD791_08945"
     CDS             10166..10975
                     /gene="dapB"
                     /locus_tag="VD791_08945"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HXV08144.1"
                     /translation="MSLVHVAVAGSSGRMGRALIESLFQAPDLRLHAALERHDSPYLG
                     KDAGEMLGTPCGVQITDDVSGALAGAHVLIDFTRPEGTLHHLQVCGERGVKMVIGTTG
                     LSPEHKARVTETAQRIAIVMAPNMSVGVTLVLKLLDSAARVLREGYDIEIIEAHHRHK
                     VDAPSGTALRMGEVIAQALGRDLGAVAVYGREGVTGERSPAAIGFATVRGGDIVGDHT
                     ALFAGVGERIEITHRASSRATFALGALRAARFLAGKKRGLYDMQDVLGLRG"
     gene            11081..12214
                     /gene="carA"
                     /locus_tag="VD791_08950"
     CDS             11081..12214
                     /gene="carA"
                     /locus_tag="VD791_08950"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HXV08145.1"
                     /translation="MPSIPPAILALADGTVFRGQSIGAEGSSAGEVVFNTSMTGYQEI
                     LTDPSYCRQIVTLTYPHIGNTGVNAEDVESDRVYAAGLVIRDLPLLASSWRRARDLGD
                     YLRAEKVPAIAGIDTRKLTRILREKGAQNGCLMAGRVDEARALAEARAFPGLAGMDLA
                     KVVSCTAPYDWDEAEWSLAKGYGSRQDGRFHVVAFDYGVKRNILRKLAGRGCRVTVLP
                     AQATAKEALAQRPDGVFLSNGPGDPEPCTYAIRAIRELLEAGTPIFGICLGHQLLGLA
                     SGARTMKMKFGHHGANHPVQDLDTGRVMITSQNHGFAVDMDTLPDNCRVTHKSLFDGT
                     LQGFARTDRPAFCFQGHPEASPGPHDVDYLFDRFIKMMMDRHR"
     gene            12323..15532
                     /gene="carB"
                     /locus_tag="VD791_08955"
     CDS             12323..15532
                     /gene="carB"
                     /locus_tag="VD791_08955"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HXV08146.1"
                     /translation="MPRRTDIHSILIIGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPGMADATYIEPVTWQMVEKVIAAERPDAVLPTMGGQTGLNCALDL
                     ARHGVLEKYGVQMIGASREAIDKAEDREKFKAAMTRIGLASPRSALAHSLEEATQVQA
                     ALGFPVVIRPSFTLGGSGGGIAYNREEFVSICERGFEASPTRELLVEESVIGWKEFEM
                     EVVRDRKDNCIIVCSIENLDPMGVHTGDSITVAPAQTLTDKEYQIMRDASIAVLREIG
                     VDTGGSNVQFAIDPRDGRMVIIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LRNDITGGATPASFEPTIDYVVTKVPRFAFEKFPQADQHLTTQMKSVGEVMAIGRTLQ
                     ESLQKALRGLETGADGLDERTTERDEIEQELAHPGPERLWYVADAFRIGLAFEEIRAL
                     THIDPWFLAQIEDIVRREQALAGVRIEQLAPAALLELKRCGFSDRRLARLLGCEQKQV
                     RERRWALGVRPVFKRVDTCAAEFDTRTAYMYSTYEEECEARPGARDKIMVLGGGPNRI
                     GQGIEFDYCCVHAALSLREDGFETIMVNCNPETVSTDYDTSDRLYFEPLTLEDVLEIV
                     AVEKPKGVIVQYGGQTPLKLARDLEANGVPIIGTSPDMIDCAEDRERFQKLLHELDLL
                     QPPNRTARDPQSALALAQEIGYPVVVRPSYVLGGRAMEIVHQQSDLERYMREAVKVSN
                     DSPVLIDRFLNDAIEVDVDAVCDGAQVLIGGIMEHVEQAGVHSGDSACSLPPYSLSAA
                     LQAELRRQTVALAKGLDVVGLMNVQYAIQEDAVYVLEVNPRASRTVPYVSKATGRALA
                     KIAARCMVGRSLAQQGVRGEVVPPYYSVKEAVFPFIKFPGVDTILGPEMKSTGEVMGV
                     GETFAEAFVKSQLAAGVKLPQTGKVFLSVRNADKPRAVEIARALIDLGFSLCATRGTA
                     AHLSEHGIDVTPVNKVMEGRPHIVDMIKNGEVAMIVNTTDERRASIQDSYSIRRSALQ
                     DRITYYTTIAGARAACVGMRQMQELRAYSVQELHGRLAA"
     gene            15555..16028
                     /gene="greA"
                     /locus_tag="VD791_08960"
     CDS             15555..16028
                     /gene="greA"
                     /locus_tag="VD791_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HXV08147.1"
                     /translation="MRIPLTSAGAAMLRAELHQLKTVERPSVIQAIQDARSHGDLSEN
                     AEYEAAKERQGFIEGRIAELERKLAKAQIIDPSHIDADGRCVFGATVELEDADSGEVV
                     TYQIVGEDEADIKRGKISITSPMGRALIGKHAGDTAEVQAPAGVRSYEILDVKYL"
     gene            16217..16669
                     /locus_tag="VD791_08965"
     CDS             16217..16669
                     /locus_tag="VD791_08965"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HXV08148.1"
                     /translation="MTRLTDGLHALAVTAWVGALWAIGLLAAPTLFAALPDRSIAGLV
                     AGRMFLYVAVLGFGCGAYLLLFRLVRFGGDAFRQGFFWVVLAMVLLSAVGEFAVQPML
                     AALKDQALPRQVMESVFRDRFAVWHGLASLVYLIQCGLGLVLVLSQDR"
BASE COUNT         2570 a         5624 c         5818 g         2799 t
ORIGIN      
        1 agctcgccgc gcaatatccg cgggtgctga atcgaatcgc cgcggtgtgg tacaagcgcg
       61 ccgaggccga tcgctgcttc gacgaactgc tgctcgacgc gcggcgcacg cgtcaaggtt
      121 tcccccaatc cgtggtctac gagatcgcgt ccctgcgcca ccaccacgtc acgcacgtgt
      181 tccccaagca cgtggatccg tgggagcaaa gccagctccg gtagcgggtt gatgcaacac
      241 gatcgcagcg cgtttccggt accacctgtc gcccgttcac gcattcgtgc gcgggtgcgc
      301 ccctcgcgag cgacgcgtgg gcaaacgacg cgtgggcgct cgcgaggtgg ttctggatca
      361 gaccgttaac cctgaaagaa ctccttcacc cgctccatcc acgacttcgc gcgcggatcg
      421 tggcggcctg actcttcctg gttgatcgcc tccagctcga gcagcagctc gcgctggcgc
      481 gcggtcaggt tgaccggcgt ctccaccacc acgtggcaga gcaggtcgcc gtgcgcgtgg
      541 ctgcgcacgc ccttgatgcc cttgccgcgc aggcggaaca cgcgcccact ctgggtttca
      601 ggcgggatct tgatcttggc gctgccgtcc agggtgggga tctcgatctc gccgcccagg
      661 gcggcggcgg tgaagctgat cggcatctcg cagtgcaggt cgttggcctc gcgctggaac
      721 accgcatggg ccttgaggcg gatcaccacg tacaggtcgc cgggcgggcc gccgttcacg
      781 ccggcttcac cctcgcccga caggcggatg cgatcgccct cgtcgacgcc ggcgggaatc
      841 ttcaccgaca gcgtcttctg ccgcttcacg cgccccgcgc cgccgcacgt ctggcacggc
      901 tcgcggatga acttgccgct gccgccgcag cgcgggcagg tctgctgcag cgaaaagaag
      961 ccctgctgga tgcgcacctg accctggccg tggcaggtcg ggcaggacac cggctcggtg
     1021 ccggccttgg cgccgctgcc gtggcaggtc tcgcaggcct cgagcgtggg gatgcggatc
     1081 ttggtctcgg tgccgcgcgc ggcatcctcg agcgtgatct cgaggttgta gcgcagatcc
     1141 gccccgcggt agacgttgga gcgctggcgg ccgccgccga agatctcgct gaagatgtcg
     1201 ccgaaggcgt cggcgaaccc gccggcttcc gcccccatcc cgaccccggc ctccacgccc
     1261 cgatggccga agcggtcgta ggcggcgcgc ttgtcggcgt cgcacaggac ctcgtaggct
     1321 tccttggcct ccttgaactt gtcttccgcc ttggggttgt ccggattgcg gtccgggtgg
     1381 tgctgcatgg ccagcttgcg ataggccttc ttgatgtctt cttccgaggc gtcgcggttc
     1441 acgccgagca cctcgtaata gtcgcgtttg gccattagct ttgagtgctt aaccgcaaag
     1501 acgcagagaa gcaaaggcgc cgcaaagaac acctattcct ttgcgcagcc tttgcgcctt
     1561 cgcgcctttg cggtgaggtt tagttacttc gctcttggcc attagctttg aatgcttaac
     1621 cgcaaagacg caaagacgca aaggcaccgc aaagaacacc tgtttctttg cgtgaccttt
     1681 gcgccttcgc gcctttgcgg tgagggtttg gttacttctt gccgtccttc acttcctcga
     1741 actcggcatc cacgacatcg ccctcctgcg ccttgccgcc ctgcccggcg ccgccggcgg
     1801 atgcatcggc gccggcgccg gccgcgccgg cgccggcctc ctgctgcgcg tacatcttct
     1861 cgcccagttt ctgcgcggcc tgggccaggg cctgggtctt ggactggatg gtgctgtggt
     1921 cgttgccctt catcgcctct tcggccgcct tgatggcgtc ctcgatgcgc gacttctccg
     1981 cggcgtccag cttgtcgccg tactcggcga gcgacttgcg cacggtgtgc accatcgcgt
     2041 cgcactggtt gcgggcgtcc accagctcgc gcgccttgcg gtcttcatcg gcatgcgact
     2101 cggcatcctt gaccatgcgc ttgatctcgt cttcggacag tcccgagctg gcggtgatct
     2161 tgatcttgtt ctccttgccg gtggccttgt ccttggccga cacgtgcagg atgccgttgg
     2221 cgtcgatgtc gaacgtgacc tcgatctgcg gcatgccgcg cggcgccggc gggatgccct
     2281 ccaggttgaa ctgaccgagg ctcttgttgc cgccggccat ctcgcgctcg ccctgcagca
     2341 cgtggatcgt caccgccgcc tggttgtcgt cggcggtgga gaacacctgg gtctgcttgg
     2401 tcggaatggt ggtgttcttc tggatcagct tggtcatcac gccgccgagc gtctcgatgc
     2461 cgagcgacag cggggtgacg tcgagcagca gcacgtcctt gacctcgccc ttgagcacgc
     2521 cgccctggat cgcggcgccc atggccaccg cctcgtccgg gttgacgtcc ttgcgcggct
     2581 ccttgccgaa gatctccttg accttgtcct gcaccttggg catgcgcgtc atgccgccca
     2641 ccaggatcac gtcgtcgatc tcggacacct tgacgccgga gtccttgatc gcggtctcgc
     2701 agggcctcgc cgtcttctcg atcaagtcct cgaccaggct ctcgaacttg gcgcgggtga
     2761 tcttcatcgc caggtgcttc ggtccggact gatccatggt gatgtagggc aggttgacct
     2821 cggtctgctg cgtcgaggac agctcgatct tggccttctc cgcggcctcc ttcagccgct
     2881 gcagcgcgag gatgtccttg cgcaggtcca cgccctggtc cttgcggaac tcgtcggcga
     2941 tgtagtccat caggcgctgg tcgaagtcct cgccgcccag gaaggtgtcg ccgttggtgg
     3001 ccagcacttc gaactgatgc tcgccctcga cctcggcgat ctcgatgatg gagatgtcga
     3061 acgtgccccc gcccaggtcg tacaccgcga tcttgcggtc gccctgcttc ttgtccagac
     3121 cgaaggcgag cgccgccgcg gtcggctcgt tgatgatgcg cttgacctcg agcccggcga
     3181 tgcggccggc gtccttggtg gcctggcgct gggagtcgtt gaaataggcg ggcacggtga
     3241 tcaccgcttc ggtgaccgtc tcgccgaggt agtcctcggc ggtcttcttc atcttcatca
     3301 gcacctgcgc cgagatctcc ggcggagcca ttttcttgcc gcgcgcctcc acccaggcgt
     3361 ccccgttggg cgcctgagag atgttgtagg gcaccagctt gatcgccttc tgcacctcgg
     3421 cgtcctggaa gcggcggccg atcaggcgct tcaccgcgta cagggtgttc ttcggattgg
     3481 tcaccgcctg gcgtttggcc ggagcaccga ccaggacctc ggcctcctcg gtgtaggcca
     3541 cgatggacgg cgtggtgcgc gaaccctcgg agttctcgat caccttgggc tgaccgttct
     3601 ccatgatggc gacacaggag ttggtcgtcc ccaggtcgat gccgatgatc tttgccattc
     3661 tgattcctcg ctctgcggat tcagggattc aaaagcggat gaagggctag gtttgggtcg
     3721 cgggcccggc tttcaagcag caggggcatg gtcgcgaccg ctccgggcgt cactgggggg
     3781 ccttggacac ggtgaccagc gccgggcgca ggacgcgatc gtgcagcagg taacccttct
     3841 gcagcacgtg caccacggtg ttggcggcct tgtcggattc gaccgtggtc atcgcctggt
     3901 gccggtgggg gtcgaacctt tcgcctaccg ggttgatttc cttgatcgcg gccttctcga
     3961 acgcgccgtc gatctgcttc agcgtgagct cgacaccctg gcgcagggcg tcgatggtgg
     4021 tgttgtccgc cgctagggcg gcctcgaggg cgtcgcgcac cggcagcagc tcggcggcga
     4081 aggtctcgag tgcgtacttg tgggcgttgg cgatgtcggc ctgggcgcgt ttgcggatgt
     4141 tgtcggcctc cgccttggcg cgcagccagg cgtcgtagtg ttcctgggcc tgcagctcga
     4201 gctggcggat gatctcctgc tggctcggca tggtttcgct ttccgccgcc ggcccgggct
     4261 cggcggcggc gggggtctcc tgggcgccgc ccggcgccgg ggaggtcagg ctaggttcct
     4321 gttcttgcat gactttcctc tattgcgagc aacccatggg cagatggggc tgcggcccgg
     4381 gatttcaaga aaggatcgag ggtccaggga aaggcgcgtt tttgccgcgc aaccccctag
     4441 atccaggcgc cggcgcgcgc cggcgtgagc catccgccga ggttgctcat cgtcaagtcg
     4501 gccagcgcct ggatccaatc gtcgcgctcg ttgaggcagg ggatggtgcg gaattcaccg
     4561 ccgccggcgg ccaggaaagc ggcccggttc tccatcgcga tctcctccag cgtctccagg
     4621 caatcggcca cgaagccggg gcagatgacg tcgacgcggc gcagcttctt cttgccgagc
     4681 gcctgcatgg tgtcgatggt gtacggctcg agccagcgtg cgcggccgaa gcgcgactgg
     4741 aaggtcgtca ggtgctgctc ggcgcgcagg cccagcgcct cggccagcag acgggcggtt
     4801 tcccggcatt cgccgtagta ggggtcgccg cggtccaccg cgaagcgggg cagcccatgg
     4861 aagctcatca ccagcaggtc cgggtgcccg tgggcggtcc agtgctcgcg cacgctgtgc
     4921 gcgagggcgg cgatgtaccc ggggtggtcg tggaagtgct tcgtgagccg cagcgccggc
     4981 gcattgcgca gccggcccag cacggcgcac accgcgtcgt acgcgctggc ggtggtgctg
     5041 gcggcgtact ggggatacag cgggatcacc agcaggcgat cgcaaccctg ctcgcgcagg
     5101 ctcgccagcg cctgcgacac cggcgggctg ccgtagcgca tcgcccactc caccgcgacg
     5161 ggacggtcga tgcgggcagc caggcgctcg cgcaggagct gcgcttgccg ccgggtgtgc
     5221 accttgagcg gcgagccctc ggcggtccag atcttcgcgt acttgtccgc cgactgccgc
     5281 ggccgcaggt tgaggatcac gccattgagg atcggccacc acagcgcggg cgggatctcc
     5341 accacccgag ggtcggacag gaactgcttc agataggggc gcaccgccgc ggcgctgggc
     5401 gcctccggcg tgcccaggtt gatcagcagc acgccgatct tggcgagcgt gccctgtggg
     5461 agcgcgggct ccgggaggta cctcactcca gcgcccgggc gcgctgcgcc agcgcgctgg
     5521 acagcagctt ggcggtgatc tccacgatcg ggatcacgcg ctcgtaggcc atgcgggtgg
     5581 gcccgatcac gcccaccgtg ccgagcacct caccgtccac ctcgtagggc gcggtgacca
     5641 cgctgcactc gtccagcggc accagccccg actcgccgcc gatgtagatc tgcacgccgt
     5701 gcgcgcggct gcccatctcc agtagctgca gcagaccggt gcgctgctcg aacaggtcga
     5761 acagccggcg caagctgagc acgttggacg acaggtcctg cacgtgcagc aagttgtgct
     5821 cgccggatat cacgtactcc tcgggctgct cggtgagggc ctcgttgccg gcctccaccg
     5881 ccttggtgag aagctgggtc atgtcgtcgc gcaggcgctc gagctcgtcg cgcaggcgcg
     5941 ccttgacctg ctcgaaggtc aggccctgat aggcctggtt gacgaagttg gccgcctcga
     6001 tcagctcgct ctgcgtgtag tcgcgctcgg tgaacagcat gcggttctgc acgtcgcctt
     6061 cgagcgtgac gatgatcagc agaacgcgct tttcggacag gcgcaggaac tccacgtggc
     6121 gaaactgggt ggagcggcgc ggcgccatca cgatcccggc gaagcgcgtc agatcggaca
     6181 gcagatgcga agccgactgc agcagccggt gcggccgctc gacgtgcagc tggccttcga
     6241 gctgatcgat ctccacccga tcgagcggct tgatcgtcaa cagactgtcg acgaagaagc
     6301 ggtagccgcg cggcgtgggc acgcggccgg cggaggtgtg cggactcgcc accagcccca
     6361 tctcctccag gtcggacatg acgttgcgga tgctggccgg cgacagatcc aggccggagt
     6421 agcgcgacag cgtgcgcgag ccgaccggct ggccctcggc gatgtagcgc tcgaccagcg
     6481 tcttgagcag tatctgtgcg cgttcgttca gcatgcgggc attctaaccg aagccgctgt
     6541 ggataaacgg ggtaaaccgc ccccggcggc cggcgctccg agccgggcat cttgtgcttt
     6601 aatgcgggcc atgccgcccc agttcaagac catcgcgctg atcggcaaat acaagagccc
     6661 cgagatcgca cagccgctgc tggagctggc gcgcttcctc gaggcccgcg gcgtgcgcgt
     6721 gttgctggat ccgctcaccg ccgcgcacgt ggcgcgcaac aacctggagg tcctgccgct
     6781 ggagcagtcg gggccgctcg ccgatctcgc cgtggtcatc ggcggcgacg gcaccatgct
     6841 caacatcgcc cgcaccctgg tcccgttcga cgtcgcgctg gtgggcgtga accaggggcg
     6901 gctcggcttc ctgaccgaca tctccaccga caacatgctg gcgaccatcg gcgccatgct
     6961 cgacggcgag ttcatcgtcg agtcgcgcat gctgctgcag tccgaggtgc gccgcgggcc
     7021 gcaagcgatc ttcgacgtgc tcgccttcaa cgacgccagc gtcgccaagg gcgccgacgg
     7081 cgggttgatc gagctggagg tgaagatcga cgagcagttc gtgtacgtgc tgcgcgccga
     7141 cgggctggtg gtgtccaccc ccaccggctc cacggcctat gcgctgtccg ccggcgggcc
     7201 gatcctgcac ccggcgctgt cggccttcgc catcgtgccg gtgtgcccgc acacgctgtc
     7261 caaccgcccc atcgccgtcg gcagcgacgc gcgcatcgag gtgctgatgc accgctgcgc
     7321 ggacgcgcgc gtgcactacg acagccattc ccactgcgag ctgcagcccg gtgaccgcat
     7381 cctggtgcgg cgctatccgc gctcgatccg cctgctgcat ccggtggggc acaactacta
     7441 ccacatgctg cgggaaaagc tccactggag cgagaccctg tagcgccgca cccggcaccc
     7501 catgctgcgg tcgctgagca tccgcgactt cgtcatcgtc gatcgcctgg acctcgagtt
     7561 ccaggacggc ttcaccgtgc tcaccggcga gaccggcgcc ggcaagtcga tcctggtcga
     7621 tgcgctcgcg ctggtgctgg gcagccgcag cgacgccgga caggtgcgcg agggggccgc
     7681 gcgcgcggag atctgcgccg agttcgcgct cgatgcgctg ccgcaggcgc aggcctggtt
     7741 gcagaccaac gatctcgccg acgaaccggg gctgtgcctg ctacggcggg tgctggaggc
     7801 gggcgggcgc tcgcgcgcct acgtcaacgg tcgcccggcg acgctgcagc agctcgagga
     7861 gctggcggag atgctggtgg acatacacgg ccagcacgag caccagtcgc tgctgcgccc
     7921 cgcggcgcag cgcgagctgc tggatgcgta cgccggggcg agtgccctgg ctcgcgacgt
     7981 ggccggggcg tggcgcgagt ggcaggaggt gcgccggcag cggctggagt gggagaagaa
     8041 cgcggacgct ctggccgccg agcgcgaacg cctgcaatgg cagcggcagg agctggaacg
     8101 gctggccttc gccgccgacg aatggggtga gctgcagctc gaccacaagc ggcttgccca
     8161 tgccgcgagc ctggtcgagg cggtggagtc cgcgctggag gcgctgtccg agggcgaggc
     8221 agcggcgctg gcggcggtga acgcggcggt gtcgcggctc aacgccgccg ccgagtacga
     8281 tccggggctg aaggagaccc tcgacgtgct ggagccggcg cagatccagt tgcaggaggc
     8341 ggtctatggg ctacgccatt accgcaaccg catcgacctc gatccgcgcc gcctgcagga
     8401 ggtcgagcag cgcctcgaag ccctgcacac gaccgcgcgc aagtaccgcg tcgcgccgcc
     8461 gcagctgccg gagctgctgg ccgatgcggc gcgccgcctc gaggagatcg gcgccggcgc
     8521 gagcccggag gcgctggcca agcgcgagcg cgaggcgcag gcgcgctacc ggacggtcgc
     8581 ggagaagctc ggcgcggagc gcgcgcgcac ggcgcaggag ctcgggcggc gcgtgaccga
     8641 gcagatgcaa gggctggcga tggccggggg acgcttcgaa gcggtgctca agcccctggc
     8701 cgagggcagc gcgcacggca tggagcagat cgagttcctg gtcgccgcca accccggcgc
     8761 cacggcgcgt tccctggcga aggtggcctc cggcggcgag ctgtcgcgca tcagcctggc
     8821 gatccagacg gtcaccagca cggtggcgcc ggtgccgacg ctggtgttcg acgaagtgga
     8881 cgccggcatc ggcgggcgcg tggccgagat cgtcggacgg atgctggcgg cgctcggcag
     8941 caagcaccag gtgatgtgca tcacgcatct gccccaggtg gcggccgcgg cgcaccacca
     9001 gtggcaggtg agcaagacgg cggcgggcaa cggcgtgcgc agcgaagtgc gcgagctaga
     9061 ccgcgcgcag cgcgtggagg agatcgcgcg catgctcggc ggggtgaaga tcaccgagac
     9121 cacgcgcaag cacgcggcgg agatgctcgg cgaaaggcgt gatcgaaaag cgtgaccggt
     9181 actgcgtgac cgaaaagcgt gacgaaaagc cgtcttcatc ctgcgcggct ggttttgtca
     9241 cgcttctcga tcacgcgttt cggtcacgcc gttcgtgacg gtcacgcctt accatgagtc
     9301 cgataggggc aattcgcctt ggtgcaatcc gcgtagatct gcagcgagtg ctcgtggatg
     9361 ttgaagccgc gctccttggc gatcttggcc tggcgccgct cgatctccgc gtcgtagaac
     9421 tcctccaccc gcccgcactg cacgcacacc agatgatcgt ggtggccgcc ctggttgagc
     9481 tcgaacaccg ccttgccggc ctcgaagtga tggcggatca ggatgccggc ctgctcgaac
     9541 tgggtcagca cccggtagat ggtggcgagc ccgatgtcca tcccctcctt gagcagcagc
     9601 ttgtagacgt cctcggccgt catgtgccgc acttcggcgg tctcgaacag gttgaggatc
     9661 ctcaggcgcg gcagcgtggc cttcaagccg atggtcttca ggtcgtgggg tttggtcatt
     9721 gcgaccctga caggcgggag tgtgccgggt atcatagccc cttcccgcac atagtgaaaa
     9781 ccgacccggt ccgaatcgcg cgatgctctt ccgcctgctt ctgctcgccg cctgcctcgc
     9841 gctgtcgtcc tgtttcctga agccccacaa gatcgatgtc cagcagggca actacctgga
     9901 cgaggagatg atcgccaagc tcaagcccgg catgacgcgc tcgcaggtgc gtttcctcct
     9961 gggcacgccg ctgatcgcgg atccatttca cccggagcgc tgggactacc tgctcctcga
    10021 ccgccgcgag ggcaagctgg tgcgcgaccg ccgcctgacg gtctggttcg agggcgacaa
    10081 gttgatccgc gccgtgaccg accagcccga cgcgccggcc aagcagcgac agcccgagca
    10141 gagcgcacaa cagacgcgct gagcgatgag cctggttcac gtcgccgtcg cgggcagctc
    10201 gggccgcatg ggccgcgccc tgatcgagag cctgttccag gctcccgacc ttcggctgca
    10261 tgccgcgttg gagcggcacg acagccctta tctcggcaag gacgccggag agatgctcgg
    10321 caccccgtgc ggcgtgcaga tcaccgacga cgtgtccggc gcgctcgccg gcgcgcatgt
    10381 gctgatcgac ttcacgcggc ccgagggcac gctccatcac ctgcaggtgt gcggcgagcg
    10441 cggcgtgaag atggtgatcg gcaccaccgg cttgagcccc gagcacaagg cacgcgtgac
    10501 cgagaccgcg cagcgcatcg ccatcgtcat ggcgcccaac atgagcgtgg gcgtgacgct
    10561 ggtgttgaag ctcctggaca gcgccgcgcg cgtgctgcgc gaaggctacg acatcgagat
    10621 catcgaggcg caccaccgcc acaaagtgga tgcgccgtcg ggcaccgcgc tgcgcatggg
    10681 cgaggtgatc gcgcaggcgc tgggccgcga cctgggcgcg gtggcggtgt acggccgcga
    10741 gggcgtgacc ggcgagcgct cgcccgccgc catcggcttt gccaccgtgc gcggcggcga
    10801 tatcgtcggc gaccacaccg cgctgttcgc cggcgtcggc gagcgcatcg agatcaccca
    10861 tcgcgccagc agccgggcca ccttcgcgct cggggcgctg cgtgccgcgc gcttcctggc
    10921 gggcaagaag cgcgggctgt acgacatgca ggacgtgctt ggattgaggg gctaggagga
    10981 tcccagctcc tcgtctataa tcacgcgtaa aagcgggagg cgtttcgagc gccgtcccgc
    11041 ttttctacgt ccgccgcctg caacgcccag gagttgagcc gtgccaagca taccgcccgc
    11101 catcctcgct ctcgcggacg ggacggtgtt tcgcggccag tcgatcgggg ccgagggcag
    11161 ctccgcgggg gaggtggtgt tcaacacttc catgaccggc taccaggaga tcctcaccga
    11221 cccctcctac tgccggcaga tcgtcacgct gacctatccg cacatcggca acaccggggt
    11281 gaacgccgag gacgtcgagt ccgaccgcgt gtacgccgcc ggcctggtga tccgcgacct
    11341 gccgctgctc gccagcagct ggcggcgcgc ccgggacctg ggcgactacc tgcgcgcgga
    11401 gaaggtgccg gccatcgccg gcatcgacac gcgcaagctc acccggatcc tgcgcgagaa
    11461 gggcgcccag aacggctgct tgatggccgg ccgggtcgat gaggcgcggg cgctggccga
    11521 ggcgcgcgcg ttccccggcc tggccgggat ggatctcgcg aaggtagtga gctgcacggc
    11581 gccgtacgac tgggacgagg ccgagtggtc gctcgccaag ggctatggca gccggcagga
    11641 cggccgattt cacgtggtgg cgttcgacta cggggtcaag cgcaacatcc tgcgcaagct
    11701 ggcgggccgc ggctgccgcg tgacggtgct gcccgcgcag gccacggcga aagaggcgct
    11761 cgcgcagcgg cccgatggcg tgttcctgtc caacggtccc ggcgatcccg agccgtgcac
    11821 ctacgccatc cgcgcgatcc gcgagctgct ggaggcgggc acgcccattt tcggcatctg
    11881 cctcggccac cagctgctcg gcctggcgag cggtgcgcgc accatgaaga tgaagttcgg
    11941 tcatcacggc gccaaccatc cggtgcagga cctcgacacc gggcgggtga tgatcaccag
    12001 ccagaaccac ggcttcgcgg tggacatgga cacgctgccg gacaactgcc gcgtcacgca
    12061 caagtcgctg ttcgacggca cgctgcaggg cttcgcccgc accgaccgcc ccgcgttctg
    12121 tttccaggga catccggaag cgagcccggg gccgcacgac gtggactatc tgttcgaccg
    12181 gttcatcaag atgatgatgg acagacatag gtgaagcagg tgattcgtga aacgtgaatc
    12241 gtgattcgtg caaaccctaa cgaatcacga atcacgaatc acgagtcacg aatcacggaa
    12301 ttcacgaatc acggctcttg acatgccaag acgcaccgac attcactcca tcctgatcat
    12361 cggcgccggc ccgatcgtca tcggccaggc ctgcgagttc gactactccg gtgcgcaggc
    12421 ctgcaaggcg ctgcgcgagg agggataccg ggtgatcctg gtgaactcga acccggccac
    12481 catcatgacc gacccgggca tggcggacgc gacctatatc gagccggtca cctggcagat
    12541 ggtggagaag gtgatcgcgg ccgagcgccc ggacgcggtg ctgcccacca tgggaggcca
    12601 gaccgggctc aactgcgcgc tcgatctggc ccggcacggg gtactggaga agtacggcgt
    12661 gcagatgatc ggcgcttcgc gcgaggccat cgacaaggcc gaggaccgcg agaagttcaa
    12721 ggcggcgatg acgcgcatcg gcctggcgag cccgcgctcc gcgctggcgc acagcctgga
    12781 agaggccacg caggtgcagg cggcgctcgg cttcccggtg gtgatccgtc cctccttcac
    12841 gctcggcggc agcggcgggg gcatcgccta caaccgcgag gagttcgttt ccatctgcga
    12901 gcgcggcttc gaggcctcgc ccacccgcga gctgctggtc gaagagtcgg tgatcggctg
    12961 gaaagagttc gagatggagg tggtgcgcga ccgcaaggac aactgcatca tcgtgtgctc
    13021 gatcgagaac ctcgacccga tgggcgtgca caccggggac tcgatcacgg tggcgccggc
    13081 gcagacgctc accgacaagg aataccagat catgcgcgac gcctcgatcg cggtgctgcg
    13141 cgagatcggc gtggacaccg gcgggtccaa cgtgcagttc gccatcgacc cgcgcgacgg
    13201 ccgcatggtc atcatcgaga tgaacccgcg cgtgtcgcgc tcctcggcgc tcgcttccaa
    13261 ggccaccggc tttccgatcg ccaaggtggc ggccaagctc gccgtgggtt acacgctcga
    13321 cgagctcagg aacgacatca ccgggggcgc gaccccggcc tccttcgagc cgaccatcga
    13381 ctacgtcgtg accaaggtgc cgcgcttcgc cttcgagaag tttccgcagg ccgaccaaca
    13441 cctcaccacg cagatgaagt cggtgggcga ggtcatggcc atcggccgca cgctgcagga
    13501 gtccctgcaa aaggcgctgc gcggtctgga gaccggcgcc gacggcctgg acgagcgcac
    13561 caccgagcgc gatgaaatcg agcaggagct ggcccacccc ggtcccgagc gcctgtggta
    13621 cgtggccgac gcgtttcgca tcgggctggc cttcgaggag atccgcgcgc tcacccacat
    13681 cgacccctgg ttcctggccc agatcgaaga catcgtgcgg cgcgagcagg cgctcgccgg
    13741 cgtgcgcatc gagcagctcg cgcccgcggc gctgctcgag ttgaaacgat gtggcttctc
    13801 cgaccggcgc ctggcgcgcc tgctcggctg cgaacagaaa caggtgcgcg agcggcgctg
    13861 ggccctgggc gtgcggccgg tgttcaagcg cgtggacacc tgcgccgccg agttcgacac
    13921 gcgtaccgcc tacatgtact cgacctacga ggaggagtgc gaggcgcgtc ccggcgcccg
    13981 cgacaagatc atggtgctgg gcggagggcc gaaccgcatc ggccagggca tcgagttcga
    14041 ctactgctgc gtgcacgccg cgctcagcct gcgcgaggac ggcttcgaga ccatcatggt
    14101 caactgcaac ccggagacgg tgtccaccga ctacgacacc tccgaccggc tctacttcga
    14161 gccgctcacg ctcgaggatg tgctcgagat cgtcgccgtg gagaagccga agggggtgat
    14221 cgtgcagtac ggcgggcaga cgccgctcaa gctggcgcgc gacctggagg cgaacggtgt
    14281 gccgatcatc ggcacctcgc cggacatgat cgactgcgcc gaggaccgcg agcgcttcca
    14341 gaaactgctg cacgagctcg atctgctgca gccgccgaac cggaccgcgc gcgatccgca
    14401 gtcggcgctg gcgctggcgc aggagatcgg ctatccggtg gtggtgcggc cgagctatgt
    14461 actgggcggg cgcgccatgg agatcgtgca ccagcaaagc gatctcgagc gttacatgcg
    14521 cgaggcggtc aaggtgtcga acgactcgcc ggtgctgatc gatcgcttcc tcaacgacgc
    14581 catcgaggtg gacgtggacg cggtgtgcga cggtgcacag gtgctgatcg gcggcatcat
    14641 ggagcacgtg gagcaggccg gcgtgcattc cggcgattcc gcctgctcgc tgccgcccta
    14701 cagcctttcg gcggcgctgc aggccgagct gcgccggcag accgtggcgc tggccaaggg
    14761 cctggacgtg gtgggcctga tgaacgtgca gtacgccatc caggaggacg ccgtgtacgt
    14821 gctggaggtg aacccgcgcg cctcgcgcac cgtgccgtac gtgtccaagg ccaccgggcg
    14881 ggccctggcc aagatcgccg cgcgctgcat ggtcggccgc agcctggcgc agcagggggt
    14941 gcgcggcgag gtggtcccgc cctactactc ggtcaaggag gcggtgtttc cgttcatcaa
    15001 gttccccggg gtggacacca tcctcggtcc ggagatgaag tccaccggcg aggtgatggg
    15061 cgtgggcgag accttcgccg aggcgttcgt gaagtcacag ctcgccgcgg gcgtgaagct
    15121 gccgcagacc gggaaggtgt tcctcagcgt tcgcaacgcc gacaagccgc gcgcggtgga
    15181 aatcgcgcgc gcgctgatcg atctcggctt cagcctgtgc gccacgcgcg gcaccgcggc
    15241 gcacctgagc gagcacggca tcgacgtgac gccggtgaac aaggtgatgg agggcaggcc
    15301 gcacatcgtg gacatgatca agaacggcga ggtcgcgatg atcgtcaaca ccaccgacga
    15361 gcggcgcgcc tcgatccagg actcctactc gatccggcgc tcggcgctgc aggaccgcat
    15421 cacctattac acgaccatcg ccggcgcgcg cgcggcttgc gtcggcatgc ggcagatgca
    15481 ggagctgcgc gcctactcgg tgcaggagct gcacggccgg ctggccgcgt gaacgcattg
    15541 cgaaggggct gagcatgaga attccgttga cttccgcggg cgccgcgatg ctgcgggcgg
    15601 agctgcacca gctcaagacc gtggagcgcc cgagcgtgat ccaggcgatc caggacgcgc
    15661 gctcccatgg cgacctgtcc gagaacgccg agtacgaggc cgccaaggag cggcagggct
    15721 tcatcgaggg ccgcatcgcc gagctggagc gcaagctcgc caaggcgcag atcatcgacc
    15781 cgtcgcacat cgacgcggat ggccgctgcg tgttcggcgc caccgtcgag ctggaggacg
    15841 cggacagcgg cgaggtggtc acctaccaga tcgtcggcga ggacgaggcc gacatcaagc
    15901 gcggcaagat ctccatcacc tcgcccatgg gccgcgcgct gatcggcaag catgcgggcg
    15961 acaccgccga ggtgcaggcg cccgccgggg tgcgctccta cgagattttg gacgtcaagt
    16021 acctgtagct gctgccgaag aaaggggtga ggcaaccaag cgaccgaacc gcgtgaaaga
    16081 ggggcgtgaa ccagaagcgt gggtgaaagg cgtcaccgag agccgtgaca agaccagggc
    16141 gtccgatgac cgggctcctc gtcatgcttt ttggtcccgc tatctggccc ggctttccgg
    16201 tgacgtcttc acctccatga ccagactcac cgacggtctc cacgcgctgg cggtcaccgc
    16261 ctgggtcggc gcgctgtggg ccatcggctt gctggcggcg cccaccctgt tcgcggcgct
    16321 gcccgatcgc agcatcgccg ggctggtggc cggccggatg ttcctgtacg tggcggtgct
    16381 ggggttcggt tgcggcgcgt atctgctgct gttccggctg gtgcgcttcg gcggcgacgc
    16441 cttccggcag gggttcttct gggtggtgct ggcgatggtc ctgctgagcg cggtcggcga
    16501 gttcgcggtg cagccgatgc tggccgccct caaggaccag gcgctgccgc ggcaagtcat
    16561 ggagagcgtg ttccgcgacc gcttcgcggt gtggcacggg ctggcgagcc tcgtttacct
    16621 gatccagtgc gggctgggcc tggtgctggt gctgagtcag gatcgctgac cccccgtttc
    16681 ggcgccggct gctccggcgc cggtcgttcc ggcgctgatt gtcccggcgt tggccgctcg
    16741 cgccagatca ccaggatcct gccgatgtgc tgcaccggcg cggcgtcgag cgccgcgcag
    16801 atgcgcgcga g
//
LOCUS       DASPGE010000279         8379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_542595, whole genome shotgun sequence.
ACCESSION   DASPGE010000279 DASPGE010000000
VERSION     DASPGE010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542595"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..690)
                     /gene="apaG"
                     /locus_tag="VD791_08970"
     CDS             complement(313..690)
                     /gene="apaG"
                     /locus_tag="VD791_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HXV08149.1"
                     /translation="MPKKYEVTVSARANYLPEQSDADEGRFAFAYTITITNTGSVAAR
                     LISRHWIITDSDNKVQEVRGLGVVGEQPLLQPQESFEYTSGAAIATPVGTMRGSYQMV
                     AEDGNSFDAPIPEFMLSMPRVLH"
     gene            783..1463
                     /gene="rpe"
                     /locus_tag="VD791_08975"
     CDS             783..1463
                     /gene="rpe"
                     /locus_tag="VD791_08975"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HXV08150.1"
                     /translation="MSSSSYRIAASILAADFARLGEEVASVVAAGADLVHFDVMDNHY
                     VPNLTFGPVVCEAIRPLTQAAIDVHLMVRPVDRIIPDFAAAGANIISFHPEATDHVDR
                     TIGAIKEAGCKAGMVLNPATPVMHLDHVLEQLDLVLIMSVNPGFGGQRFIPYTLDKLR
                     AVRSRIERTGRDIWLEVDGGVKVDNIASIAAAGADTFVAGSAIFGTPDYNATIAAMRA
                     ALAKPNAV"
     gene            1460..2143
                     /locus_tag="VD791_08980"
     CDS             1460..2143
                     /locus_tag="VD791_08980"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:NF009695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HXV08151.1"
                     /translation="MKRFSVDAAAIDLDGTLLDTLPDIAEAARRMLRELGRPPAEDDA
                     VRACIGDGIPRLVKRLLTGQRDGEPESAAFARALALFERHYRDTFLQRPVPFPGVVDG
                     LAQLCAAGLKLACVTNKSEAFALPLLAAAGIAHRFDLVVGGDSLPARKPDPLPLRHVA
                     GRFGLDPGRLLVIGDSDNDTRAARAAGCPVLCVPYGYRGAGEVRDLDCDAIVLDLLHV
                     CALITPNRS"
     gene            2273..3769
                     /gene="trpE"
                     /locus_tag="VD791_08985"
     CDS             2273..3769
                     /gene="trpE"
                     /locus_tag="VD791_08985"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HXV08152.1"
                     /translation="MTESEFRQFAAQGFNRIPLVLETFADLDTPLSVYLKLANRPWSY
                     LLESVQGGERFGRYSFIGLPAETRFEVRGHACSEYRHDELVQQAQHADPLEFVRACQA
                     RFHAALPRGLPRFVGGLVGFFAYDTVRYIEGRLDRPDKPDVPDKPDPLATPDILLLLS
                     EQLAVVDNLSGKLYLIVFADPRGPNAFERAQIRLRELAARLRTPVQVPDEIPMQPGVV
                     QNEFSEAAFLAAVERAKRYIYDGDIMQVVLSQRVSHPYPASPLALYRALRALNPSPYM
                     FYYDMGGFHVVGASPEILVRLEGDTVTLRPIAGTRPRGATREEDLQLERDLLADPKER
                     AEHVMLMDLGRNDVGRVAQAGSVRVTENMVVERYSHVMHIVSNVEGRLKPGLDAIAVL
                     RASFPAGTVSGAPKVRAMEIIDELEPSKRGVYAGAVGYLGFNGDMDLAIAIRTAVVKD
                     GRLYVQAGAGIVADSVPANEWTETQSKARAVLRAAEMAQAGLNTPRPS"
     gene            3799..4374
                     /locus_tag="VD791_08990"
     CDS             3799..4374
                     /locus_tag="VD791_08990"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HXV08153.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGEEVVVFRNDQVSLEEIEALNP
                     AQVVISPGPCTPDEAGISVPAIRRFAERIPILGVCLGHQSIGRAFGGRIVHAKQLMHG
                     KTSPIHHLDTGVFRGLKEPLVATRYHSLVIERASLPPELEITAWTDDGEIMGVRHRSL
                     PVEGVQFHPESILTEQGHELLRNFLGGTRQH"
     gene            4403..5428
                     /gene="trpD"
                     /locus_tag="VD791_08995"
     CDS             4403..5428
                     /gene="trpD"
                     /locus_tag="VD791_08995"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HXV08154.1"
                     /translation="MTPQDALVRVIEHREIFHEEMVSLMRQIMSGEVSPTLIAAIIVG
                     LRVKKETIGEIAAAAQVMREFATRVEVADHAHLLDTCGTGGDAAHTFNISTAAAFVAA
                     AAGAKVAKHGNRSVSSKSGSADVLEALGVSIGLNPGQVARSIGEVGVGFMFAPHHHPA
                     MKHAAPVRRELGVRTLFNILGPLTNPAGARNQVMGVFHPDLVGIQTRVLQRLGSRHVM
                     VVYGLEGLDEISISGETMVGELKDGEVREYNVHPRQFGMEATDIRAIQVSDAQQSAEL
                     LRAALEGKPGPARDIVALNAGAAIYVSGLTPSLEEGVEKASSIMAGAGARRKLEELVR
                     FTNSFRE"
     gene            5435..6229
                     /gene="trpC"
                     /locus_tag="VD791_09000"
     CDS             5435..6229
                     /gene="trpC"
                     /locus_tag="VD791_09000"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HXV08155.1"
                     /translation="MAGSDILQRILAVKRGEVAAARAAKPLELLRREAQAAPAARDFV
                     GALRAKIGSGQAAVIAEIKKASPSKGVLRVDFRPAEIARSYAQHGAACLSVLTDREFF
                     QGDAAHLAEARAASGLPALRKDFMLDAYQMYETRAMGADCILLIVAALDLPAMLDLEA
                     AAGELGMATLVEVHDAAELDRALRLRTPLVGINNRNLRTFETRLETTLALLERVPQER
                     IVVTESGILAPPDVALMRGHGVHAFLVGEAFMRASIPGAELSRLFA"
     gene            6457..7674
                     /locus_tag="VD791_09005"
     CDS             6457..7674
                     /locus_tag="VD791_09005"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HXV08156.1"
                     /translation="MKKTLMALAVGAAFVAPAALADVTISGAINMGIAYTDSDDGDAS
                     AGNSITGAVGTSSGGSNYQLSSNYSNITISSVDDLGGGLKLDFAAQMDWGGINTAGGG
                     ITNRNSHIGLVSESWGGVWYGTNENLYERYFYTVDPLDGAAGVGGNLVMLGTPGYGTV
                     FDNGQAGCTAAAGCAGFYRRTDQTIWYDSPSFGGLTFGIYTTTTAFENFGPGGTNPIV
                     MGGGAKWVGQGLPLQVWGAIEMHDDLFGLNAITGVGGATGSDDQAIQVGVGYTLGDIF
                     IFANYEMLEYESDGLAVGQVNKYERDAASIGVKWNLASGYVGAQYIMAMEGDCELQGG
                     GACNADDSDAQMIAAGYYHTMSKQTQAYVVGAWVDNGDLASYVATAGGISTNNNAPGA
                     DHLTISVGLKHSF"
     gene            complement(7799..>8379)
                     /locus_tag="VD791_09010"
     CDS             complement(7799..>8379)
                     /locus_tag="VD791_09010"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXV08157.1"
                     /translation="PIIPFITEELWQKVAPLAGKSGESSMIAPYPRAEPAKVDPEATR
                     QVALLKALTNACRTLRSEMNVAPSQRIPLLIAGDAAAVRPAAPYIEFLARLSGIEFTG
                     AELPRADAPVAVAGDFRLMLKIEVDRAAECERLSKEAQRLQAEIAKAQSKLANTAFVD
                     RAPAQVVAQERERLATFTRTLEQVQAQLGKLS"
BASE COUNT         1319 a         2827 c         2899 g         1334 t
ORIGIN      
        1 aggtgatcgg cgggagcacg ggcacctcgg ggggcttctc ggccaccggg agtttctcgg
       61 cgaccgggtc ggggcaggga agcgccgctt gcacggccgg cggcggcgcc tccggtacac
      121 tggcgcaggc ggcgagcgca aatgcgagtg cggcgagacc ggtgcgaccg gctcggacca
      181 gaggacaacg ggcaatgctg tggcttgcgt tggagatcgt gctggggctg gcgctgttca
      241 tcggcatcgt ctggtggacc ttcccgcgcg cgccgcgcgt tcgggcgaag caaccgccgc
      301 ggaattgagg cgtcagtgga gcacgcgcgg catgctcaac atgaactccg ggatgggcgc
      361 gtcgaagctg ttgccgtcct cggccaccat ctggtagctg ccgcgcatgg tgcccaccgg
      421 cgtggcgatc gccgcgccgc tggtgtactc gaagctctcc tgcggctgca gcagcggctg
      481 ctcgcccacc acccccaggc cgcgcacctc ctgcaccttg ttgtccgagt cggtgatgat
      541 ccagtggcgg ctgatgagac gcgcggcgac cgagccggtg ttggtgatgg tgatggtgta
      601 ggcgaacgcg aagcgcccct cgtccgcatc cgactgctcg ggcaggtaat tggcgcgcgc
      661 gctcaccgtg acctcgtact tcttcggcat ggcgcgattg cacacgattt tgccgcgact
      721 gacaagccgc ggcgaccgac gacacgagag atgcggcgtc gttttgccgc tacaatcgcg
      781 ccatgagcag cagcagctat cgcatcgccg ccagcatcct cgccgccgac ttcgcgcgcc
      841 tgggcgagga ggtggcgagc gtggtcgcgg ccggcgccga cctggtgcac ttcgacgtga
      901 tggacaacca ctacgtgccc aacctcacct tcgggccggt ggtgtgcgag gcgatccgcc
      961 cgctgaccca ggcggccatc gacgtgcacc tcatggtgcg gccggtggat cgcatcattc
     1021 ccgacttcgc cgcggccggc gccaacatca tcagcttcca tcccgaggcc accgaccacg
     1081 tcgaccgcac catcggcgcg atcaaggaag ccggttgcaa ggccggcatg gtgctcaatc
     1141 ccgccacccc ggtgatgcat ctggaccacg tgctggagca gctcgatctg gtgctgatca
     1201 tgtcggtgaa cccgggcttc ggcggccagc gcttcatccc ttacaccctg gacaagctgc
     1261 gcgccgtgcg cagccgcatc gagcgcaccg ggcgcgacat ctggctggag gtggacgggg
     1321 gcgtgaaggt ggacaacatc gcgtcgatcg ccgctgccgg cgccgacacg ttcgtcgccg
     1381 gatcggccat cttcggcacg cccgactaca acgccacgat cgcggccatg cgcgccgcgc
     1441 tggccaagcc gaacgcggtt tgaagaggtt ctcggtcgac gccgccgcga tcgatctcga
     1501 cggcacgctg ctcgacaccc tgcccgacat cgccgaagcc gcccggcgca tgctgcgcga
     1561 gctcgggcgt ccacccgcgg aggacgacgc ggtgcgcgcc tgcatcggcg acggcattcc
     1621 gcgcctggtc aagcgcctgc tcaccggcca gcgcgacggg gagcccgaat cggctgcctt
     1681 cgcacgcgcg ctggcgctgt tcgagcgcca ctaccgcgac actttcctgc agcggccggt
     1741 accgttcccg ggggtcgtgg acgggcttgc gcagctgtgc gccgccggcc tcaagctcgc
     1801 ctgcgtgacc aacaagtccg aagcctttgc cctgccgttg ctggccgccg ccggcatcgc
     1861 ccaccgcttc gacctggtgg tcggcggcga cagcctgccc gcgaggaagc ccgacccgct
     1921 gcccttgcgc cacgtcgccg ggcgcttcgg gctcgacccc gggaggctgc tggtgatcgg
     1981 cgattccgac aacgacacgc gcgcggcgcg ggcggccggc tgcccggtgt tgtgcgtgcc
     2041 gtacggctac cggggcgcgg gggaggtgcg ggatctggat tgcgatgcta tagttctcga
     2101 cctgctccat gtctgtgcat tgataacacc caaccggtcg tgaccaccga gactctgccg
     2161 acgcgacggc tcaagctccc ggcatcgggc acctggcgct attggcgccg cgtgaccgtt
     2221 gcctgacctt gccgtccgtt cgcagttttc agagtcttcg ctggagccgt ccatgaccga
     2281 gtccgaattc cggcagttcg ctgcccaggg cttcaatcgc attccgctgg tcctcgagac
     2341 cttcgccgac ctcgacacgc cgctgtcggt ctatctcaag ctcgccaacc ggccctggtc
     2401 ctacctgctc gagtcggtgc agggcggtga gcgcttcggc cgctattcgt tcatcggcct
     2461 gccggcggag acccgcttcg aggtgcgcgg ccatgcctgc agcgagtacc ggcacgacga
     2521 gctcgtgcag caggcgcagc acgcggatcc gctcgagttc gtgcgcgcct gccaggcccg
     2581 cttccacgct gcgctgccca ggggcctgcc gcgcttcgtc ggcggactgg tcggcttctt
     2641 cgcttacgac acggtgcgct acatcgaggg gcgcctggac cgaccggaca agcccgacgt
     2701 ccccgacaaa cccgatcccc tggctacgcc ggacatcctg ctgctgctgt cggagcaact
     2761 ggcggtggtg gacaacctgt ccggcaagct ctatttgatc gtgttcgccg atccgcgcgg
     2821 gccgaacgct ttcgagcgcg cgcagatccg gctgcgcgag ctggcggcgc ggctgcgcac
     2881 cccggtgcag gtgcccgacg agatcccgat gcagcccggc gtggtgcaga acgagttctc
     2941 ggaggcggcg ttcctggcgg cggtggagcg cgcgaagcgc tacatctacg acggcgacat
     3001 tatgcaggtg gtgctgtccc agcgcgtcag ccatccctat ccggcctcgc cgctggcgct
     3061 gtaccgggcg ctgcgcgcgc tgaatccctc gccctacatg ttctactacg acatgggcgg
     3121 tttccacgtg gtcggcgcct cgcccgagat cctggtcagg ctggaaggcg acaccgtcac
     3181 gctgcggccg atcgccggca cgcgcccgcg cggcgccacg cgcgaggagg acctgcagct
     3241 cgagcgcgat ctgctcgccg acccgaagga gcgcgccgag cacgtcatgc tgatggacct
     3301 gggccgtaac gacgtcggcc gcgtggcgca ggcgggcagt gtgcgcgtga ccgagaacat
     3361 ggtggtggag cgctactcgc acgtgatgca catcgtgtcc aacgtcgaag gccggctcaa
     3421 accggggctg gatgcgatcg ccgtgctgcg cgccagcttc ccggccggca ccgtgagcgg
     3481 cgcgccgaag gtgcgcgcga tggagatcat cgacgagctg gagccgagca agcgcggcgt
     3541 ctacgccggc gcggtcggct atctcggctt caacggcgac atggacctcg ccatcgccat
     3601 ccgcaccgcg gtggtcaagg acggccgcct gtacgtgcag gccggtgccg gtatcgtcgc
     3661 ggactcggtg cccgcgaacg aatggacgga gacgcagagc aaggcgcgcg cggtgctgcg
     3721 tgcggccgag atggcgcagg cggggttgaa cacgccccga ccgagttgag caggtcgaac
     3781 gggacggagg acagagcgat gctgctgatg atcgacaact acgactcgtt cacctacaac
     3841 ctggtgcagt atttcggcga gctgggcgag gaggtggtgg tgttccgcaa cgaccaggtc
     3901 tcgctcgaag agatcgaggc gctcaacccg gcgcaggtgg tgatctcgcc cgggccgtgc
     3961 acgcccgacg aggccgggat ttcggtgccc gccatccggc gcttcgccga gcgcatcccg
     4021 atcctcggcg tgtgcctcgg ccatcagagt atcggccggg cgttcggcgg gcgcatcgtg
     4081 cacgccaagc aactcatgca cggcaagacc tcgccgatcc atcatctcga caccggggtg
     4141 ttccgcggac tgaaggagcc gctggtggcc acccgctacc actctctggt catcgagcgc
     4201 gcgtccctgc ccccggagct cgagatcacc gcctggaccg acgacggtga gatcatgggg
     4261 gttcgccacc gctcgctgcc ggtggagggc gtgcagttcc acccggagtc gatcctgacc
     4321 gagcaggggc acgagctgct gcgcaacttc ctgggcggga cgcggcaaca ctgaagcggt
     4381 cggcgagcgc aggggaggga acatgacacc gcaagacgcg ctggtgcgcg tgatcgagca
     4441 ccgcgagatc ttccacgagg agatggtgtc gctgatgcgc cagatcatga gcggcgaagt
     4501 gtcgccgacg ctgatcgccg ccatcatcgt cgggctgcgc gtgaagaagg agaccatcgg
     4561 cgaaatcgcg gccgcggcgc aggtgatgcg cgaattcgcc acccgcgtcg aggtcgccga
     4621 ccacgcgcac ctgctcgaca cctgcggcac cgggggcgac gccgcccaca ccttcaacat
     4681 ctcgaccgcg gcggcgttcg ttgccgccgc cgccggggcc aaagtggcca aacacggcaa
     4741 ccgctcggtg tccagcaagt cgggcagcgc cgacgtgctg gaggcgctgg gcgtgagcat
     4801 cggcctcaat cccggccagg tggcgcgctc gatcggcgag gtgggcgtgg gcttcatgtt
     4861 cgcgccgcac catcacccgg ccatgaagca cgcggccccg gtgcggcgcg agctgggggt
     4921 gcgcacgctg ttcaacatcc tgggaccgct caccaacccg gcgggcgccc gcaaccaggt
     4981 gatgggtgtg ttccacccgg acctggtggg gatccagacc cgggtcctgc agcggctcgg
     5041 cagccggcac gtgatggtgg tgtacggcct cgaaggcctg gacgagatct cgatctcggg
     5101 cgagaccatg gtgggcgagc tgaaggacgg cgaagtgcgc gagtacaacg tgcacccgcg
     5161 ccagttcggc atggaggcaa ccgatatccg cgcgattcag gtgtccgatg cgcagcagtc
     5221 ggccgagctg ctgcgggccg cgctggaagg caagccgggc ccggcgcgcg acatcgtggc
     5281 gctcaacgcc ggcgccgcga tctacgtgtc cgggctgacg ccgtcgctcg aggagggtgt
     5341 cgagaaggcg tcctcgatca tggccggcgc cggcgcgcgg cgcaagctgg aggagctggt
     5401 gcgcttcacc aactccttcc gggagtagga gccggtggcc ggtagcgaca tcctgcagcg
     5461 catcctggcg gtcaagcgcg gggaggtggc ggcggcgcgg gcggccaagc cgctggagct
     5521 gctgcgccgt gaagcgcagg ctgcgccggc cgcgcgcgac ttcgtcggtg cgctgcgcgc
     5581 gaagatcggc tcgggccagg ccgccgtgat cgccgagatc aagaaggcga gcccgagcaa
     5641 gggcgtcctg cgcgtcgatt tccgtcccgc cgagatcgcg cgcagctatg cgcagcacgg
     5701 tgccgcgtgc ctgtccgtcc tgacggaccg ggagttcttc cagggcgatg ccgcccatct
     5761 cgccgaggcg cgcgccgcga gcggcctgcc ggcgctgcgc aaggacttca tgctggacgc
     5821 ctaccagatg tacgagacgc gcgccatggg cgccgactgc attctgctga tcgtcgccgc
     5881 gctcgacctg ccggcgatgc tcgacctcga ggccgccgcc ggcgagctgg gcatggccac
     5941 gctggtcgag gtgcacgacg cggccgagct ggaccgcgcg ctgcgcctgc gcacgccgct
     6001 cgtggggatc aacaaccgca acctgcgcac gttcgagacg cgcttggaaa cgacgctcgc
     6061 gctgctcgag cgcgtgccgc aggagcgcat cgtcgtcacc gagtcgggca tcctcgcgcc
     6121 ccccgacgtg gccttgatgc gcgggcacgg ggtgcacgct ttcctcgtcg gcgaggcctt
     6181 catgcgtgcc tcgattcccg gggcggaact gagccgcctt ttcgcctgat tgctgctttg
     6241 caacaattta gcgctttctg ttgcgccaac gcaacactta ggcgcgaaag ccgcataaag
     6301 cgttgcagaa agcttgctca cacgcgttgc gctttggcag aatcgccaac gccctctcac
     6361 agggagaagt acgtgcgcac gggttccggg cacagcggca taccgcccgg cttctcaaca
     6421 agaatctgag acaagggttc gtttcaagga gatggtatga agaagacttt gatggctctg
     6481 gccgtcggcg cggcgttcgt ggcgcccgcg gccctcgctg atgtcaccat cagcggcgcg
     6541 atcaacatgg gtatcgccta caccgattcg gacgacggcg acgcaagtgc cggcaactcg
     6601 atcactggcg cagtcggaac gagcagcggc ggttcgaact accagctgtc aagcaactac
     6661 tccaacatca cgatctcgtc ggtcgacgat ctgggtggcg gcctgaagct ggacttcgcg
     6721 gctcagatgg actggggcgg catcaatact gcaggcggcg gcatcaccaa ccgcaacagc
     6781 cacatcggcc tggtgagcga gagctggggc ggcgtctggt acgggacgaa cgaaaacctg
     6841 tacgagcgct acttctacac ggtcgacccg ttggacggcg ccgcgggtgt cggcggtaac
     6901 ctcgttatgt tgggcacgcc gggctacggc acggtgttcg acaacggcca ggcgggttgc
     6961 actgcggctg ccggctgcgc gggcttctat cgccgcaccg accagaccat ctggtacgac
     7021 agcccgagct tcggcggcct caccttcggc atctatacga ccacgaccgc attcgagaac
     7081 ttcggtccgg gcggcaccaa tccaatcgtc atgggcggcg gcgccaagtg ggtgggtcaa
     7141 ggcttgccgc tgcaggtctg gggcgcaata gagatgcacg acgacttgtt cggcttgaac
     7201 gccattacgg gtgtcggtgg cgccaccggc tcggatgacc aggccatcca ggtcggcgtc
     7261 gggtatacgc tcggcgatat cttcattttc gccaactatg aaatgctcga gtacgagtcc
     7321 gacggcttgg cggtgggtca agtgaacaag tacgagcgcg acgccgcctc gatcggtgtg
     7381 aagtggaacc tcgccagcgg ctacgtcggc gcgcagtaca tcatggcgat ggaaggcgac
     7441 tgcgagctcc agggcggcgg cgcctgcaac gccgacgaca gcgacgcgca gatgatcgct
     7501 gcgggctact accacaccat gtcgaagcag acccaggctt acgtcgtggg cgcctgggtg
     7561 gacaacggcg acctcgcgtc gtatgtcgct acggctggtg gcatcagcac caacaacaac
     7621 gcacccgggg cagaccacct gaccatcagc gtgggcctga agcacagctt ctaacagcga
     7681 cagcaacacg cggttcgaac gggcgccttc gggcgcccgt ttttcttgca aagccggagg
     7741 cgcgacgagt aaattcccct ctccccttgt gggcggatga ggggttccct agaggttctc
     7801 agctgagctt gccgagctgc gcctgcacct gctccagcgt gcgggtgaag gtcgccaggc
     7861 gctcgcgctc ctgcgcgacg acctgcgcgg gggcgcggtc gacgaaggcg gtgttggcca
     7921 gcttggactg tgccttcgca atctcggcct gcaggcgctg cgcctccttg gagagccgct
     7981 cgcactcggc cgcgcgatcc acttcgatct tcagcatcag acggaagtca ccggccaccg
     8041 cgaccggcgc gtcggcccgc ggcagctcgg cgccggtgaa ctcgatgccc gacagccgcg
     8101 ccaggaactc gatgtagggc gcggcgggcc gcaccgcggc ggcgtcgccc gcgatgagca
     8161 gcgggatgcg ctgcgacggc gcgacgttca tctcgctgcg cagcgtgcgg caggcgttgg
     8221 tcagcgcctt gagcagcgcc acctggcgcg tggcctccgg atccaccttg gccggctctg
     8281 cccgcggata gggcgcgatc atgctgctct cgccggactt gcccgccagc ggcgccacct
     8341 tctgccacag ctcctcggtg atgaacggga tgatcgggt
//
LOCUS       DASPGE010000280         2760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1361365, whole genome shotgun sequence.
ACCESSION   DASPGE010000280 DASPGE010000000
VERSION     DASPGE010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361365"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VD791_09015"
     CDS             <1..702
                     /locus_tag="VD791_09015"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV08158.1"
                     /translation="DNYERFFTSFVYTELFLKSLLYALITTAGCLLMAYPLALLIARS
                     PKSQRDLLVLLVILPFWSNFLIRVYAWVIILGPQSVFVGAFNWAIGLAGLGPVRLLYT
                     PAAVVIGLVYVHLPFMVLPLYANLEKHDPALLDAAQDLGAGAWQRFWRVTFPLSLPGV
                     YAGAALVFIPAFGIFAIPDILGGTSGAMIGTVIKQQFLDSRDWPFGSVLSMVLTVVAV
                     ALAAVAAWFARRSRS"
     gene            699..1469
                     /locus_tag="VD791_09020"
     CDS             699..1469
                     /locus_tag="VD791_09020"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV08159.1"
                     /translation="MRRSAWLAAAAVAGYAFLYAPLAIVVAYSFNDSTMNAQWVGFTL
                     DWYRKLFRNQDMLVAARNSLVIGLTASAVATALGTMAGYAMYRYRLRLLPLLVLAPIA
                     IPEILMGVSLLLFFVMINLTLGMLSITLAHIAFCIGFVAIVVRARLAGLDESLVEAAR
                     DLGATPWQAFRLVTLPLIMPGVVAGALMAFTLSIDDFVITFFTAGPDSRTLPLAIYTM
                     VKITVTPEVNAVSTLLMLLTLVLILVASRLSPGAMRGR"
     gene            1540..2607
                     /locus_tag="VD791_09025"
     CDS             1540..2607
                     /locus_tag="VD791_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HXV08160.1"
                     /translation="MRLAKLLLVLAAVGLGACGKKDEVLSLYNWNQYIAPETVERFEK
                     ACGCKVQQAFYSDNSELMAKIAGGAKGYDVLVPTSNYVSDLANGGYLQPIEKSKLPNL
                     KNIMPAYLDTEFDPGNQYSVPYAYTITMLGYNEEKLSELGVPVDSWALIFDPKLLEKI
                     KGRVTVLDSPDELTAAALKYLGYSANAHDPAKLEEAKQVIVKAKPYWAAFKASGYIEL
                     LAAGDIWVAHGYSNDIYQADLGAQQAGKSFRIKHAMPKEGAVLALDAMVVAKDAPHPE
                     LAHRFIDFMLEGQNSADLTNMLGSGNPNSAAMEFIKPEIKSMAAVFPDAETAEKLEML
                     KPMDADQRRQLNQVWTEIKAE"
BASE COUNT          436 a          907 c          921 g          496 t
ORIGIN      
        1 gacaactacg agcgtttctt caccagcttc gtgtacaccg agctgttcct gaagtcgctg
       61 ctgtacgcgc tgatcaccac cgccggctgc ctgctgatgg cctacccgct ggcgctgctg
      121 atcgcgcgca gcccgaagag ccagcgcgat ttgctggtgc tgctggtgat cctgccgttc
      181 tggagcaact tcctgatccg ggtgtatgcc tgggtcatca ttctcggtcc gcagtcggtg
      241 ttcgtgggcg ccttcaactg ggcgatcggg ctcgccggcc tggggccggt gcgactgctg
      301 tacacgccgg cggcggtggt gatcgggctg gtctatgtgc acctgccgtt catggtgctg
      361 ccgctctacg ccaacctgga gaagcacgat ccggcgctgc tcgatgccgc ccaggacctg
      421 ggtgcgggcg cctggcagcg cttctggcgc gtcaccttcc cgctgtcgct gccgggcgtg
      481 tacgccggtg ccgcgctggt gttcattccc gccttcggca tctttgccat ccccgacatc
      541 ctcggcggaa ccagcggcgc catgatcggt accgtgatca aacagcagtt cctcgacagc
      601 cgcgactggc cgttcggcag cgtgctgtcg atggtgctga cggtggtggc cgtcgcgctg
      661 gcggccgtgg ccgcgtggtt cgcgcggaga tcgcgctcat gaggcgctcg gcctggctgg
      721 cggccgcggc cgtcgccggc tacgcgttcc tgtacgcacc gctcgccatc gtggtggcgt
      781 actcgttcaa cgattcgacc atgaacgcgc agtgggtggg gttcacgctg gactggtatc
      841 gcaagctgtt ccgcaaccag gacatgctgg tcgccgcgcg taactccctg gtgatcggcc
      901 tcacggcgag cgctgtggcc accgcgctcg gcacgatggc cggctacgcc atgtatcgct
      961 accgcctgcg cctgctgccg ctgctggtgc tggcgccgat cgcgatcccc gagatcctga
     1021 tgggcgtgag cctgctgctg ttcttcgtca tgatcaacct cacgctcggc atgttgtcga
     1081 tcaccctggc gcatatcgcg ttctgcatcg gtttcgtggc gatcgtggtg cgcgcgcgcc
     1141 tggccggcct ggacgagagc ctggtcgagg cggcgcgcga cctcggcgcc acgccgtggc
     1201 aggcgttccg gctggtcacg ctgccgctga tcatgcccgg cgtggtggcc ggtgcgctga
     1261 tggcgttcac gctgtcgatc gacgacttcg tgatcacctt cttcaccgcc gggcccgatt
     1321 ccaggacgct gccgctcgcc atctacacca tggtcaagat caccgtcacg ccggaggtca
     1381 acgccgtgtc cacgctgctg atgctgctca cgctggtgct gatcctggtg gcctcgagac
     1441 tgtcgccggg agccatgcgc ggccgctgag gcgaggcgcg cgccgtgcag gcgtttgccg
     1501 tatccttgcg ccacccttga gactaccgga ggctgcggga tgagactggc gaaactgctc
     1561 ctggtgttgg cggccgtggg cctgggcgca tgcggcaaga aagacgaggt gctgagcctg
     1621 tacaactgga accagtacat cgcgcccgag acggtggagc gcttcgagaa ggcctgcggc
     1681 tgcaaggtgc agcaggcgtt ctactcggac aactcggagc tgatggcgaa gatcgccggc
     1741 ggcgccaagg gctacgacgt gctggtgccc acctcgaact acgtgtcgga tctcgccaac
     1801 ggcggctacc tgcagccgat cgagaagtcc aagctgccga acctgaagaa catcatgccg
     1861 gcctacctcg ataccgagtt cgaccccggc aaccagtact cggtgccgta tgcctacacc
     1921 ataacgatgc tgggctacaa cgaggagaag ctcagcgagc tgggcgtgcc ggtggactcg
     1981 tgggccctga tcttcgatcc caagttgctg gagaagatca agggccgggt gaccgtgctc
     2041 gacagccccg acgagctgac cgcggcggcg ctgaaatacc tgggctactc ggccaacgcg
     2101 cacgacccgg ccaagctgga ggaggccaag caggtgatcg tcaaagccaa gccgtactgg
     2161 gccgcattca aggcctccgg ctacatcgag ctgctggccg ccggcgacat ctgggtcgcg
     2221 cacggctact cgaacgacat ctaccaggcc gacctcggcg cccagcaggc gggtaagagc
     2281 ttccgcatca agcacgccat gcccaaggag ggcgcggtgc tcgcgctcga tgccatggtc
     2341 gtcgccaagg acgcgcctca tccggaactg gcgcacaggt tcatcgattt catgttggag
     2401 gggcagaact ccgccgacct gaccaacatg ctcggctcgg gcaacccgaa cagcgcggcg
     2461 atggagttca tcaagccgga gatcaagtcg atggcggcgg tgttccccga tgcagagacg
     2521 gccgagaagc tggagatgct caaaccgatg gacgccgatc agcgccggca actcaaccag
     2581 gtctggaccg agatcaaggc cgagtgaccg gtacgcggga cccccgccgt gcgcggtctt
     2641 ccgacgcgct ctagcgctcg gtgaagctgg cccgaaacgc ggtcaggaag ggcccgagct
     2701 cgttctcggg cagggcgttg atcccgcctg ccccaggacg cccgcccccg gtggggaagg
//
LOCUS       DASPGE010000281         5364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1597156, whole genome shotgun sequence.
ACCESSION   DASPGE010000281 DASPGE010000000
VERSION     DASPGE010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597156"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VD791_09030"
     CDS             complement(<1..562)
                     /locus_tag="VD791_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV08161.1"
                     /translation="MLTQTALESRTRRGFAAAPWSAGADLFAGLGAQRARGALRIGLL
                     VPTSGAAGIWGPSTIACARLAAQELNQAGGVLGREVQLHVVDAASENRALTQQSARLL
                     RERAIDAVVGMHLSSVRKALLPVIGGRLPYVYTPLYEGGERHPGVFTLGETPAHQLVP
                     ALAALSEHRTPVRWALIGNDYVWPRVS"
     gene            661..1083
                     /locus_tag="VD791_09035"
     CDS             661..1083
                     /locus_tag="VD791_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007740423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXV08162.1"
                     /translation="MAHPPITTSLTYLLAQANRLVSAALERLLAAEGAQIEHWRVLEV
                     LSDEQGRSMGELAQLVLMNHPALTKLIDRMVSRGWVHRAADAADSRRVLVFITDGGLD
                     FVARLRARVAEYQESIDAGDRRAVQLKRLLGTLIREHG"
     gene            1235..2197
                     /gene="arsS"
                     /locus_tag="VD791_09040"
     CDS             1235..2197
                     /gene="arsS"
                     /locus_tag="VD791_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012732795.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HXV08163.1"
                     /translation="MHATLPLLQHTTFPAIRRRRLDTLQVNLGYKCNQTCVHCHVNAG
                     PNRKEMMQRETIDLVLHFLRAAGVLHLDITGGAPELNPHFRHLVSEARAMDVHVIDRC
                     NLTVLEVAGQEDTAAFLAAKRVEITASLPCYLEDNVDRQRGDGVFQSSIRVLRRLNDL
                     GYARPESGLTLNLVYNPQGPTLPPAQGKLEQDYEKFLGERHGIRFDRLFTLANMPIQR
                     FGSTLVSKGQFGAYMRLLKNAHRDENLDTVMCRSLVSVDWQGYVYDCDFNQMLHLPLR
                     VAGRPRVHLASLIGRDLEGNPIAVADHCFGCTAGQGSSCGGALT"
     gene            2214..4361
                     /locus_tag="VD791_09045"
     CDS             2214..4361
                     /locus_tag="VD791_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXV08164.1"
                     /translation="MTRSRLALLLVLAALVAAFFVFDLGRFFSLEWFKSQQAAIEAFH
                     RNHPWQTAAALFLIYVAVTGLSLPGALPLSLAAGAIFGLLWGTILVSFASSIGATLAF
                     LASRFLFRQAIHRRFGDKLRAIDAGMEKEGPFYLFTLRLVPLFPFFMINLAMGLTSIR
                     TWTFYWVSQLGMLAGTMVYVNAGTQIAQIESLRGILSPGLLLSFALLGVFPLIAKNAV
                     NWLRARKVYARWPRPARFDRNLVVIGAGSAGLVSAYIAAAVKAKVTLIEKHRMGGDCL
                     NTGCVPSKALLRSAKLLSQIGRAQQWGIRSAQAQFDFAEVMERVQRVIRQVAPHDSVE
                     RYTALGVECIEGEAKIVSPWSVEVADAAGKRTLTTRAIVIAAGAQPFVPPIPGLDRVG
                     YLTSDTLWELRVLPGRLLVLGGGPIGCELAQAFARFGAKVTQVEMLPRILMREDPEIS
                     QMVAERFRAEGIDVRVGHRAKEFRVEDGEKLLIAEHDGRDVRIAFDQVLVAVGRVANT
                     TGYGLEELGIPVTKQRTVEVNQHMATLYPNIYACGDVAGPYQFTHTASHTAWYAAVNA
                     LFGRFRRFRADFSVIPWATFTDPEVARVGLNETEAKEKGIACEVTTYGIDDLDRAIAD
                     GEAHGLVKVLTVPGKDRILGATIVGEHAGDLIVEYIAAMRHGIGLNKILGTIHIYPTL
                     AEANKYAAGNWKRAHAPQALLRWVERYHAWMRG"
     gene            4370..5059
                     /locus_tag="VD791_09050"
     CDS             4370..5059
                     /locus_tag="VD791_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HXV08165.1"
                     /translation="MARLSIVMPVLNEATEIVGALERLQPLRARGHEVIVVDGGSGDD
                     TMRLAAPLADRVLQAPRGRARQMNAGAGHARGDVLLFLHADTRLPQDADRLIAEPLAG
                     SAAAWGRFDVAIDGRHPLLRVIAAAMNLRSRATGICTGDQGIFVHRALFRAIGGYADI
                     ELMEDIALTRLLRRHAPPACPARRAVTSGRRWERRGVWRTMALMWWLRLRYFLGASPA
                     RLRRLYEGERT"
     gene            5061..>5364
                     /locus_tag="VD791_09055"
     CDS             5061..>5364
                     /locus_tag="VD791_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV08166.1"
                     /translation="MTGERILVQVFAKAPMPGEVKTRLIPVLGREGATELYCRLVQHT
                     LATVAMARVGSIELWTTAAPRNEEFLRARERLLGIPLLPQPDGDLGARMSAAATHGL"
BASE COUNT          806 a         1841 c         1877 g          840 t
ORIGIN      
        1 gggacacccg gggccacacg taatcgttgc cgatcagcgc ccagcgcacc ggcgtgcgat
       61 gctcgctcaa cgccgccagg gccggcacca gctgatgcgc gggcgtctcg ccgagcgtga
      121 acacaccggg atggcgctcg ccgccttcgt agagcggcgt gtagacgtaa ggcagccgcc
      181 cgccgatcac cggcaacagc gccttgcgca cgctgctcag gtgcatgccg acgacggcgt
      241 cgatcgcacg ctcccgaagc aggcgcgccg actgctgcgt cagcgcccgg ttctcgctcg
      301 cggcatcgac cacgtgcagc tgcacttccc gccccaagac gccgccggcc tgattcaact
      361 cctgcgcggc cagccgggcg caggcgatgg tggaggggcc ccagatcccg gccgcaccgc
      421 tggtcgggac caacaggccg atgcgcaatg ccccgcgggc gcgctgcgcc cccagccccg
      481 cgaacagatc cgcgccggcg ctccagggcg ctgcggcgaa tccgcggcgg gtcctcgatt
      541 cgagtgcggt ctgtgtgagc atgggtgccc gccgtgctgg ctcgtcaagc gagcgccggc
      601 aggtcgattg gacggcgtgg catttataat aggccaaact tcctccgcgc aggagcacgc
      661 gtggcacatc ccccgatcac gacctcgctg acctatctgc tggcccaggc gaaccggctg
      721 gtgtcggccg cgctggaacg gctgctggcg gccgaaggcg cgcagatcga gcactggcgg
      781 gtgctggaag tgctgtccga cgagcagggc cgctcgatgg gcgagctggc gcagctggtg
      841 ttgatgaatc atccggcgct caccaagctc atcgaccgga tggtcagccg tggctgggtg
      901 caccgggcgg cggatgccgc cgacagccgc agggtgctgg tgttcatcac cgacggcggg
      961 ctcgacttcg tcgcccggct gcgcgcgcgc gtggccgagt accaggagtc catcgatgcc
     1021 ggcgaccggc gcgccgtgca gctcaagcgc ctgctgggca cgctgatccg cgagcacggc
     1081 tgagccgtcg ggtgccgaag caggcggcag tacgagtggg ctcggcggtt tgcatccgga
     1141 ccgggctggg cggcgtaaca agcaggacga gtgtcgacag gcgcgccgta atcctgtcac
     1201 actccctgcg acaaagagtc gagcgaccgc cgccatgcac gccaccctcc cgctgctgca
     1261 gcacaccacc ttccccgcca tccggcgccg ccgcctggat acccttcagg tcaacctggg
     1321 ttacaagtgc aaccagacct gcgtgcactg tcacgtcaat gccggcccga atcgcaagga
     1381 gatgatgcag cgcgagacca tcgatctggt gctgcacttc ctgcgcgcgg cgggcgttct
     1441 gcacttggac atcaccggcg gcgcgcccga gctgaacccg cacttccgcc atctggtgag
     1501 cgaggcccgg gcgatggatg tgcatgtcat cgaccgctgc aatctcacgg tgctggaggt
     1561 tgcgggacag gaggacaccg ccgccttcct cgccgccaag cgggtcgaga tcacggcctc
     1621 gctgccctgc tacctggagg acaacgtcga ccggcagcgc ggcgacgggg tgttccagtc
     1681 gagcatccgc gtgctgcgca ggctgaacga cctgggctac gcccggcccg agagcggtct
     1741 cacgctcaac ctggtgtaca acccgcaggg tccgacgctg cctccggcgc aaggcaagct
     1801 cgagcaggac tacgagaaat tcctcggcga gcggcacggg atccggttcg atcgcctgtt
     1861 cacgctcgcc aacatgccga tccagcgctt cggcagcacg ctggtgtcca agggccagtt
     1921 cggcgcctac atgcgcttgc tcaagaacgc ccaccgggac gagaacctgg acaccgtgat
     1981 gtgccgctcc ctcgtctcgg tggactggca gggctacgtc tacgactgcg acttcaacca
     2041 gatgctgcac ctgccgttgc gcgtcgccgg ccgcccgcgc gtgcatctcg cctcgttgat
     2101 cgggcgcgac ctggaaggca atcccatcgc cgtggccgac cattgcttcg gctgcaccgc
     2161 cggacagggg tcgagctgcg gcggcgcgct cacctgaacg ccggtgcacg cccgtgaccc
     2221 gtagccgcct ggcgctgctg ctggtgctcg cggcgctcgt ggcggcgttc ttcgtcttcg
     2281 acctcggccg cttcttcagc ctcgagtggt tcaagtcgca gcaggcggcg atcgaagcct
     2341 tccatcgcaa ccatccctgg cagacggcgg cggcattgtt cctgatctac gtcgcggtca
     2401 ccgggctgtc gctgcccggc gcgctgccgc tgtcgctggc ggccggcgcg atcttcgggc
     2461 tgctgtgggg aacgatcctc gtctcgttcg cctccagcat cggcgcgacg ctggctttcc
     2521 tcgcttcgcg cttcctgttc cgccaggcga tccatcgccg cttcggtgac aagctgcgcg
     2581 cgatcgatgc gggcatggag aaggaggggc ctttttatct gtttaccctg cggctggtgc
     2641 cgctgttccc gttcttcatg atcaacctgg cgatgggtct gacctccatc cgcacctgga
     2701 cgttctactg ggtcagccag ctcggcatgc tcgccggcac catggtgtac gtgaacgcgg
     2761 gcacgcagat cgcgcagatc gagtccctgc gcggcatcct ctcgccgggg ctgctgctgt
     2821 cattcgccct gctgggcgtc tttccgctga tcgcgaaaaa tgccgtgaac tggctccggg
     2881 cgcgcaaggt gtacgcgcgc tggccgcggc ccgcgaggtt cgaccgcaac ctggtggtga
     2941 tcggcgcggg ctccgccggc ctggtgtccg cgtacatcgc cgccgcggtc aaggccaagg
     3001 taacgctgat cgagaagcac cgcatgggcg gcgattgcct gaataccggc tgcgtgccgt
     3061 ccaaggcgct gctgcgctcg gccaagctgc tgtcgcagat cgggcgcgcg cagcaatggg
     3121 gcatccgttc ggcgcaggcg cagttcgact tcgccgaggt gatggagcgc gtgcagcgcg
     3181 tgatccgcca ggtcgcgccg cacgactcgg tggagcgcta cacggcgctg ggcgtggagt
     3241 gcatcgaggg cgaggcgaag atcgtctcgc cgtggagcgt ggaggtggcc gacgccgcgg
     3301 gcaaacgcac gttgactacc cgcgccatcg tcatcgccgc gggtgcgcag ccgttcgttc
     3361 cgccgattcc cggtctggac cgggtcggct atctcacctc cgatacgctg tgggaactgc
     3421 gcgtcctgcc cgggcggctg ctggtgctgg gcggcggccc gatcggctgc gagttggcgc
     3481 aggccttcgc gcgcttcggc gccaaggtga cgcaggtcga gatgctgccg cggatcctga
     3541 tgcgggaaga cccggagatc tcgcagatgg tggcggagcg cttccgtgcc gaggggatcg
     3601 acgtgcgcgt aggtcacagg gccaaggagt tccgcgtcga agacggcgag aagctgctga
     3661 tcgcggagca cgacgggcgc gacgtgcgca tcgccttcga ccaggtactg gtcgcggtgg
     3721 ggcgggtggc gaacaccacc ggctacgggc tggaagagct cggcatcccg gtgaccaagc
     3781 agcgcaccgt cgaggtgaac cagcacatgg ccacgctcta tcccaacatc tacgcctgcg
     3841 gcgacgtcgc cgggccgtat cagttcaccc acaccgcctc acacaccgcc tggtacgccg
     3901 cggtcaacgc cctgttcggc cgcttccggc gcttccgcgc cgacttctcg gtgatcccct
     3961 gggccacgtt caccgacccg gaggtggcgc gcgtgggcct gaacgaaacc gaagccaagg
     4021 aaaagggcat cgcctgcgaa gtcaccacct acggcatcga cgacctggac cgcgccatcg
     4081 cggacggcga ggcgcatggg ctggtgaaag tgctcaccgt gccgggcaag gaccgcatcc
     4141 tgggggccac catcgtcggc gagcacgccg gcgacctgat cgtcgagtac atcgcggcga
     4201 tgcggcacgg catcgggctc aacaagatcc tgggcacgat ccacatctac ccgacgctgg
     4261 ccgaggccaa caagtacgcc gcgggaaact ggaagcgcgc ccacgcgccg caggcgctgc
     4321 tgcgctgggt ggagcgctat cacgcctgga tgcgcgggta acgggttaaa tggcacgcct
     4381 gtcgatcgtg atgccggtgt tgaacgaagc gaccgagatc gtcggcgcgc tggaacgttt
     4441 gcagccgctg cgcgcgcgcg ggcacgaggt gatcgtggtg gacggcggca gcggggacga
     4501 tacgatgcgg ctggccgcgc cgctcgccga ccgcgtgctg caagcgccgc gcgggcgcgc
     4561 ccggcagatg aatgccgggg cggggcacgc gaggggcgat gtgctgctgt tcctgcacgc
     4621 ggacacccgt cttccccagg acgcggaccg gttgatcgcc gagccgctgg cgggcagcgc
     4681 ggctgcgtgg ggacgcttcg acgtcgcgat cgacggccgc cacccgctgc tcagggtgat
     4741 cgccgccgcc atgaacctgc gctcgcgcgc cacgggaatc tgcaccggcg accagggcat
     4801 cttcgtgcat cgggcgctgt tccgggccat cggtggatac gcggacatcg agctgatgga
     4861 agacatcgcg ctcaccaggc tgctgcgccg tcacgcgccg ccggcatgcc cggcacggcg
     4921 cgcggtaacc tccggccgcc gctgggagcg gcgcggcgtg tggcgcacca tggcgctgat
     4981 gtggtggctg cggctgcggt atttcctcgg cgcctcgccg gcgcggctgc gccggctcta
     5041 cgaaggcgag cgcacgtgag atgaccggcg agcgcatcct ggtgcaggtg ttcgccaagg
     5101 ctcccatgcc gggcgaagtg aagacgcgtc tgatcccggt gctgggccgc gagggcgcta
     5161 ccgagctgta ttgccgcctg gtgcagcaca cgctcgcaac ggtggcgatg gcgcgcgtgg
     5221 gcagcatcga gctgtggacc accgcggcgc ctcggaacga ggagttcctg cgtgcccgcg
     5281 agcgcctgct cggcatcccg ctgctgccgc agcccgacgg cgacctgggt gcacgcatga
     5341 gcgccgcggc gacgcatggc ctgg
//
LOCUS       DASPGE010000282         8468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1010699, whole genome shotgun sequence.
ACCESSION   DASPGE010000282 DASPGE010000000
VERSION     DASPGE010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8468
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010699"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VD791_09060"
     CDS             complement(<1..201)
                     /locus_tag="VD791_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08167.1"
                     /translation="MAYEQGTSGSDVLQGGSGSDCLSGGGGNDVLNGGGGNDAVYGGS
                     GNDVLSGGTGNDLLNGGTGNDVL"
     gene            complement(377..3832)
                     /locus_tag="VD791_09065"
     CDS             complement(377..3832)
                     /locus_tag="VD791_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXV08168.1"
                     /translation="MTERDPAPVPDAAQRSRALDTRRSFIVQAPAGSGKTELLIQRAL
                     ALLAQVQAPEEIVAITFTRKAAGEMRARILQALESAATETPPGAAHEHTTWRLARAAL
                     QRDAQAGWGIGASPNRLRIHTIDALCAALARQMPLLSDFGALPKMVEDADGLYREAAR
                     RTLALLHEPEDAATPMARAAARPAGKAVAQVLAHLDNDLHNGELLLADMLRSRDQWIR
                     HVADRDSPRLERPTLEAGLAGVIRDALQGLRSLVPAALQDDLLALARFAAANLLRDGR
                     SSLIVVCAGLTALPGAEPEDLTAWRGLTELLLTRDGEPRKQADAKLGFPPPTSEKKNR
                     VLAAQLQRAKDRHEALVNALAGQPELVDALQRVRTLPPPAYTDAQWATLDAVTALLPV
                     AMAQLALVFGERGRLDFTAVAQAAVRALGSDEEPTDLALALDYRISHLLVDEFQDTSQ
                     SQYELLTRLTAGWQADDGRTLFVVGDPMQSIYRFREAEVALYLRARRQGIGGVALEPL
                     TLGVNFRSQAGLVSWVNGAFRSLLPHNEDVESGAVPFVSSQPLHPALEGPAVQVHPLL
                     ENDRDAEARAVLEIVQAARREDAQGSIAILVRSRTHLAAIVPALREAGLRFQAIEIEA
                     LAHRPVVQDLRVLTRALLHPADRIAWLAVLRAPWCGLTVADLDALARPDRRACLWELM
                     DRPALLAQLSEDGRARLVQLRETLRHALAQRRRTGLRRWIEGAWLALGGPAAARDEAD
                     LEDAQVFLGLLDELERGGDLPDFAELDEHIAKLYALPDPAAAAGLQVMTIHKAKGLEF
                     DVVVLPGLGYAPQATAPRLLRWLERPRAQGRADLLLAPIREPSQDSDPIYRYLDSLDT
                     AQGEHEDGRLLYVAATRAKRRLHLLGHANLNKEGAPTPASRCLLQRLWPVLRGAFEAA
                     AEQKQRPDAAQPSAPVAPTTVLQRLAPGWRLPAPEPVAAQAPGRSPFGELGMVEPVEF
                     SWASETARHIGTAVHRALLRIAQDGIANWSAARVSAMRNLFARDLIELGVPAAQLDMA
                     LDRVTSALGLTLEDQRARWILSPHADAASELRLTGVVDGAIVNLAVDRTFVDERGIRW
                     VVDYKTGRHEGGSLETFLEREQQRYRAQLERYATVLHNLDRRPVRMALYFPLLQAWRE
                     WGADC"
     gene            complement(3825..6611)
                     /locus_tag="VD791_09070"
     CDS             complement(3825..6611)
                     /locus_tag="VD791_09070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXV08169.1"
                     /translation="MDHDLLRQLESGATLVTPNRRLARDLARRFNLVQIGTGRSAWPS
                     ADVLPWSAWLERTLGEAARDDPRLRLLGPAQELALWQRLIEPSHAGSPLLDAAAAARS
                     ASAARRLQLAWRIDLRPAAGGLPQDARTWLAWARGFEAACREGAWLEPARVPDALAVR
                     VRAGGARLPPLLILYGFEPLSPQEQVLLEALRAAGTRVAVRDADLVEGSVRRAPYLTA
                     EEEWAQVARRARALLEADAAQRIGVVVPELSLRRDTVLRIFDETLDPGRLLPGASRRS
                     AACNVSLGVPLSAYPLVHTALAVLRLAHGRLALTEAGALLRSPFLAAAEHEFLRRALL
                     DAQLRRLGRLEVEPADVLELARGRSPDDPSACPQLAQRLAAWIPRAQEAQTLRQPPSA
                     WSATFLSLLAGLGWPGERTIDSDEYQTWDKFRDTVAGLAHLDGVLDRLRYPQALAWLA
                     RLTADTLFQPESEEVPVQILGALESVGLQFDHLFVTGLHDEAWPQPARPNPFLPVAVQ
                     RAHGVPHASAEWELGFAHRMTALWRGGAPQVILSYPTREGDRTLRPSPLLAGLAAAQD
                     APAASRAYAQRIREHGRLERISDAVAPPLRAGLDAPATVEVPAAVDVLGGAAVLQDQA
                     ACPFRAFAVHRLGARALEEGHPGLDARERGTLLHRALALLWGELRSQRRLLSLSEERL
                     LDVVSSAVDGAIASIARRRPDAFGVAFTELERARLRRLLLALLELERRRAPFEVVARE
                     EPRTLELGGLRLQARVDRIDALDDGRRVILDYKTGKAGTGEWDGDRPDSPQLPLYAVT
                     DAGDLAALAFVVLRAEEIAFKGLGAEAGLLPGVEPLERPQTWGARLTQWRDVLKALAA
                     QFVAGHAPVAPKRYPHTCEYCALGALCRVKELFDPGPVSVEQEPMPLEQEPVPLEQEP
                     ISQEPISGEPDRDD"
     gene            complement(6692..7015)
                     /gene="fdxA"
                     /locus_tag="VD791_09075"
     CDS             complement(6692..7015)
                     /gene="fdxA"
                     /locus_tag="VD791_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HXV08170.1"
                     /translation="MTYVVTESCIKCKYTDCVDVCPVDCFREGPNMLVIDPDECIDCT
                     LCVAECPVEAIFAEDDVPVAQRDFIALNAELSKEWPTITEKKPAPPDAGEWDKVMPKK
                     QLLER"
     gene            7179..7505
                     /gene="trxA"
                     /locus_tag="VD791_09080"
     CDS             7179..7505
                     /gene="trxA"
                     /locus_tag="VD791_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HXV08171.1"
                     /translation="MSEHIHHVTDDTFESEVLQSATPVLVDYWAEWCGPCKMIAPILD
                     EVAQEYAGRLKIAKLNIDENQGTPPKFGIRGIPTLMLFKNGNMEAMKVGALSKSQLTA
                     FIDSNI"
     gene            7741..>8468
                     /gene="rho"
                     /locus_tag="VD791_09085"
     CDS             7741..>8468
                     /gene="rho"
                     /locus_tag="VD791_09085"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HXV08172.1"
                     /translation="MHLSDLKNLHVTELVEMANANEIENANRLRKQDLIFALLKNQAK
                     KGESIFGEGTLEVLPDGFGFLRSPDTSYLAGPDDIYVSPSQIRRFNLHTGDSIEGEIR
                     TPKDGERYFALVKVDKVNSEPPENAKNKILFENLTPLHPTNVMILERDIRAEENLTGR
                     IIDIVAPCGKGQRGLIVSPPKAGKTVMLQHIAHAITSNHPDVMLIVLLIDERPEEVTE
                     MQRSVRGEVVSSTFDEPATRHVQVA"
BASE COUNT         1297 a         3094 c         2881 g         1196 t
ORIGIN      
        1 gaggacatcg ttgccggtgc cgccgttcag cagatcgttg ccggtgccgc cgctcagaac
       61 gtcgttgccg ctgccgccgt ataccgcgtc gttgccgccg ccgccgttca gaacgtcgtt
      121 gccgccgccg ccgctgaggc aatcgctgcc ggatccgcct tgcagcacgt cgctgccgga
      181 tgtgccctgc tcgtaagcca tgtgtgtcgt ccccgtggat gtaacttctt gttagcgtct
      241 ccccccgggt ccgcgcatta tcggacgccg aagccgacaa ggcattcaat gtcaaagata
      301 tgtgcgcaga aaaatgccgg atgaatcgcg tgccgacgcg gtttgcgcgg ccggtcgcgg
      361 cgctacgcgg attgtgtcaa caatctgcgc cccactcccg ccacgcctgc agcagcggga
      421 agtacagcgc catgcgcacc ggccggcggt cgagattgtg cagcactgtt gcatagcgct
      481 cgagctgcgc gcggtagcgc tgctgctcgc gctcgaggaa agtctccagg ctgccgcctt
      541 cgtgcctgcc ggtcttgtag tcgacgaccc agcgaatgcc gcgctcgtcg acgaaagtgc
      601 ggtcgacggc gagattgacg atcgcgccgt cgaccacgcc ggtcaggcgc agctccgagg
      661 ccgcatccgc gtgcggtgag aggatccagc gtgcacgctg gtcctccagc gtgaggccga
      721 gcgcgctcgt cacccgatcc agcgccatgt ccaattgcgc ggccggcaca cccagctcga
      781 tcaggtcgcg cgcgaacagg ttgcgcatcg cgctcacgcg cgccgcgctc cagttcgcaa
      841 tgccgtcttg cgcgatgcgc agcagcgccc ggtgcacggc ggtaccgatg tggcgtgcgg
      901 tttccgaagc ccaggagaac tccaccggct ccaccattcc cagctcgccg aagggcgacc
      961 ggcccggcgc ctgcgcagcc acgggctctg gtgccggcag gcgccagccc ggcgccaagc
     1021 gctgcaagac cgtagtgggt gcgacgggcg cggacggttg tgctgcatcc gggcgctgct
     1081 tctgctccgc cgccgcttcg aacgcgccgc gcagcaccgg ccacaagcgc tgcagcaggc
     1141 agcgcgacgc gggcgttggg gcgccttcct tgttgaggtt ggcgtggccc agcagatgca
     1201 agcgccgttt cgcgcgcgtg gccgccacgt acagcaagcg gccgtcctcg tgctcgccct
     1261 gcgcggtgtc gagcgagtcg agatagcgat agatcggatc ggagtcctgg ctcggttcgc
     1321 ggatcggtgc cagcagcaga tcggcgcggc cctgcgcgcg cgggcgctcc agccagcgca
     1381 gcagccgcgg cgcagtcgcc tgcggtgcgt agcccaaccc cggcaggacc acgacgtcga
     1441 actcgaggcc cttggccttg tggatcgtca tcacctgcag gcccgcagcc gcggccggat
     1501 cgggcagcgc gtacagcttc gcgatgtgct cgtcgagctc ggcgaagtcg ggcaggtcgc
     1561 cgccgcgctc gagctcatcg agcagtccca ggaacacctg cgcatcctcg agatcggcct
     1621 cgtcccgcgc tgccgccggc ccgcccagcg ccagccaggc accctcgatc cagcgccgca
     1681 gcccggtgcg ccggcgctgc gcgagcgcgt ggcgcagcgt ctcgcgcaac tgcaccaagc
     1741 gggcgcggcc gtcctccgaa agctgcgcca gcagcgcagg ccggtccatc agctcccaca
     1801 agcaggcgcg ccgatcgggg cgggcgagcg cgtcgagatc ggccaccgtc aagccgcacc
     1861 agggcgcgcg cagcaccgcc agccaggcga tgcgatcggc gggatgcagc aacgcccgcg
     1921 tgagcacgcg caggtcctgc accacggggc gatgcgcgag cgcttcgatc tcgatggcct
     1981 ggaagcgcag gcccgcctcc ctgagcgccg gcacgatggc cgccagatgg gtacggctgc
     2041 gcaccaggat ggcgatgctg ccctgcgcgt cctcgcggcg cgcagcctgc acgatctcga
     2101 gcacggcgcg cgcctcggcg tcgcggtcgt tctccagcaa agggtgcacc tgcaccgccg
     2161 gcccttccag cgcagggtga agcggctgcg aggacacgaa cggcaccgcg ccgctttcga
     2221 cgtcttcgtt gtgcggcagc aggctgcgga acgcgccgtt gacccagctc accagccccg
     2281 cctgcgagcg gaagttgacg ccgagtgtga gcggctcgag cgccacgccg ccgataccct
     2341 ggcgccgggc gcgcagatac agcgccacct ccgcctcgcg gaagcggtag atcgactgca
     2401 tcggatcgcc caccacgaac agggtgcggc cgtcgtccgc ctgccagccc gcggtgagcc
     2461 gcgtcagcag ctcgtattgc gactgggacg tgtcctggaa ctcgtccacc agcagatggc
     2521 tgatgcggta gtcgagcgcg agcgccaggt cggtgggctc ctcgtcgctc cccagcgcgc
     2581 gcaccgccgc ctgcgccacg gcggtgaaat ccagccgccc gcgctcgccg aacaccagcg
     2641 cgagctgcgc catggcaacc ggcaacagcg cggtgacggc atcgagcgtc gcccattgcg
     2701 catcggtgta cgcgggcgga ggcagcgtgc gcacgcgctg cagggcgtcc accagctcgg
     2761 gctgcccggc gagcgcgttc accaacgcct cgtggcggtc cttggcacgc tgcagttgcg
     2821 ccgcgaggac ccggttcttc ttctccgagg tcggcggcgg aaaccccagc ttcgcatccg
     2881 cttgcttgcg cggctcgccg tcccgggtca gcagcagttc ggtcaggccg cgccaggccg
     2941 tcaggtcttc gggctccgcg ccgggcagcg ccgtcagccc ggcgcagacc acgatcaggg
     3001 aggagcgccc gtcgcgcagc agattggcag ccgcgaagcg cgccagcgcc aggagatcat
     3061 cctgcagcgc tgcaggcacc agggagcgca ggccttgcag cgcatcgcgg atcactccgg
     3121 ccaggccggc ctccagagtg gggcgctcca accgcggaga atcgcgatca gcgacatgcc
     3181 gtatccactg gtcgcggctg cgcagcatgt ccgccaggag cagctccccg ttgtgcagat
     3241 cgttgtccag gtgcgccagc acctgcgcca ccgcttttcc cgccggccgt gccgccgccc
     3301 gcgccattgg cgtggccgcg tcctcgggct cgtgcagcag cgcgagcgtg cgccgcgcgg
     3361 cttcgcggta cagcccatcc gcgtcctcca ccatcttcgg cagcgcgccg aagtcggaca
     3421 gcagcggcat ctggcgcgcg agcgccgcgc acagcgcgtc gatggtgtgg atgcgcaggc
     3481 gattggggct cgcgccgatc ccccaccccg cctgtgcatc gcgctgcaac gctgcgcgcg
     3541 cgagccgcca cgtggtgtgc tcatgcgcgg cgccgggcgg cgtctcggtc gcggccgact
     3601 cgagcgcctg caggatgcgc gcgcgcatct cgcccgcggc cttgcgcgtg aaggtgatcg
     3661 ccacgatctc ctccggcgcc tgcacctgcg ccagcagcgc gagcgcgcgc tggatcagca
     3721 gttcggtctt gcccgaaccg gcgggagcct gcacgatgaa cgagcggcgc gtgtccagcg
     3781 cgcgactgcg ctgcgcagcg tccggaaccg gcgccggatc gcgctcagtc atcgcggtcc
     3841 ggctccccgg agatcggttc ttgggagatc ggttcttgct ccagcgggac gggctcctgc
     3901 tccaacggca tcggctcttg ctccaccgac accgggcccg ggtcgaacag ctccttgacc
     3961 cggcacagcg cgcccagcgc acagtactcg caggtgtgcg ggtagcgctt cggcgcgacc
     4021 ggcgcgtggc cggcgacgaa ctgcgcggcg agggctttca ggacatcgcg ccattgcgtc
     4081 aggcgtgcgc cccaggtttg cggccgctcc agcggctcca cgcccggcag caaccccgcc
     4141 tcggcgccga gccccttgaa cgcgatctcc tcggcgcgca gcaccacgaa tgcaagcgcg
     4201 gcgagatcgc ccgcatcggt cactgcgtac agcggcagct gcggcgagtc gggccgatcg
     4261 ccgtcccact ctccggtgcc ggccttgccc gtcttgtagt cgaggattac gcgccggcca
     4321 tcgtccagcg cgtcgatgcg atccacgcgc gcctgcaggc gcagcccgcc cagctccagc
     4381 gtgcgcggct cttctcgcgc caccacctcg aacggtgcgc gccggcgctc cagctcgagc
     4441 agcgccagca ggagccggcg caagcgcgcg cgctccagct ccgtgaacgc gacgccgaag
     4501 gcgtcgggcc gccgccgcgc tatcgacgcg atcgccccgt ccaccgcgct cgacacaacg
     4561 tcgagcagcc gctcctcgga cagcgacaac aggcgtcgct gcgagcgcag ctccccccag
     4621 agcagcgcca gcgcccggtg cagcagcgtt ccccgttctc gcgcatccag ccccgggtgc
     4681 ccctcctcca gcgcacgggc cccgagccgg tgcaccgcga acgcgcggaa cggacacgcg
     4741 gcctggtcct gcaggacagc ggcgcccccc aatacgtcga ccgcagcggg tacctcgacc
     4801 gtggcgggcg cgtcgaggcc ggcgcgcaaa ggcggtgcga cggcatcgct gatgcgctcc
     4861 agccggccat gctcgcgaat gcgctgtgcg taggcgcgtg aggcggctgg cgcgtcctgc
     4921 gccgccgcaa gcccggccag cagcgggctc gggcgcaacg tacggtcgcc ctcgcgggta
     4981 ggatagctga ggatcacctg cggcgcgcca ccgcgccaca gcgccgtcat gcggtgtgcg
     5041 aaacccagct cccactcggc gctcgcgtgc ggcacgccgt gcgcacgttg cacggcaacc
     5101 ggcaggaacg ggttggggcg cgcgggctgc ggccacgcct cgtcgtgcaa tccggtgacg
     5161 aaaagatgat cgaactgcag tcccaccgac tccagcgcac ccaggatctg caccggcacc
     5221 tcctcggact cgggctggaa cagcgtgtcg gcggtgaggc gtgcgagcca tgccagcgcc
     5281 tgcggatagc gcaggcggtc gagcacgcca tccagatgcg ccagcccggc cacggtgtcg
     5341 cggaacttgt cccaggtctg atactcgtcg ctgtcgatcg tgcgctcacc cggccagccg
     5401 aggccggcga gcagcgacag gaaggtggcg ctccaggcag acggcggctg gcgcagggtc
     5461 tgcgcctcct gcgcgcgcgg aatccaggcc gcgagccgct gcgcgagctg cgggcaggcg
     5521 ctcgggtcgt cgggtgaacg gccacgcgcc agctccagca cgtcggccgg ctccacctcg
     5581 agccgcccga gccggcgcag ctgtgcatcc agcagggcgc gccgcaggaa ctcgtgctcc
     5641 gcggcggcca ggaaagggct gcgcagcagc gcacccgctt cggtgagcgc cagccggccg
     5701 tgcgccaggc gcagcaccgc gagcgccgtg tgcaccagcg gataagcgga caacggcacg
     5761 cctagagaga cgttgcacgc cgccgaccgg cgcgacgctc cgggcagcag gcgccccggg
     5821 tcgagcgtct cgtcgaagat gcgcagcacc gtgtcacgcc gcaaggaaag ctcgggcacc
     5881 accacgccga tgcgctgtgc agcgtcggct tcgagcaacg cgcgcgcgcg ccgcgccacc
     5941 tgtgcccact cttcttcggc ggtgaggtac ggcgcacggc ggacgctgcc ttcgaccagg
     6001 tcggcgtcgc gcaccgcgac gcgcgtgccg gccgcccgca gcgcctcgag cagaacctgc
     6061 tcctgggggc tgagcggttc gaagccgtac aggatcagca gcggcggcag gcgcgcgccc
     6121 cccgcgcgaa cgcgtacggc cagcgcgtcc ggcacccgcg ccggttccag ccaggcgccc
     6181 tcgcggcagg ccgcctcgaa cccgcgcgcc cacgcgagcc aggtgcgcgc gtcctgcggc
     6241 aggccgcccg ccgcgggccg cagatcgatg cgccaggcga gttgaagccg gcgggcggcg
     6301 ctcgcgctgc gtgcagcggc ggcagcgtcc agcagcggcg atccggcgtg ggagggctcg
     6361 atcagacgct gccacagcgc cagctcctgc gccgggccca gcaggcgcag ccgcggatcg
     6421 tcgcgggccg cctcgcccag ggtgcgttcg agccaggcgc tccacggcag cacgtcggcg
     6481 ctgggccagg cgctgcgtcc cgtgccgatc tggacgaggt tgaagcggcg ggcgagatcg
     6541 cgcgccaggc gccggttggg tgtaaccagc gtggcgccgg actcgagctg gcgcagaaga
     6601 tcgtggtcca cggcgcccgt ctccttggaa aaagcgggag gcgtgtggcc gccgagcgcg
     6661 agatcggacg cagccggcgg gatgcttcgc ctcagcgctc gagcagttgc ttcttcggca
     6721 tcaccttgtc ccactcgccg gcgtcgggcg gtgctggctt cttctcggtg atcgtcggcc
     6781 actccttgga cagctcggcg ttgagcgcga tgaagtcgcg ctgggcgacc ggcacgtcat
     6841 cctcggcgaa gatcgcctcc accgggcact cggctacgca cagggtgcag tcgatgcact
     6901 cgtccgggtc gatcaccagc atgttgggcc cctcgcggaa gcaatccact gggcacacgt
     6961 ctacgcagtc ggtgtacttg cacttgatgc aggattcggt gacgacgtag gtcatgtcct
     7021 ggggaccggt gcatgaatcg gcgtaaaatt ctagcagaat gggtctgcag ggccgcggcc
     7081 gtcgcctttc cacgacaggg cgtttttggg ttagcattcg ccccacagag tccttgagcc
     7141 gcccccgggg gcggcgcctc actttcccca atctctccat gagcgagcat atccatcacg
     7201 tcaccgacga cacgttcgaa tccgaagtgc tgcagtccgc cactcccgtg ctggtggact
     7261 actgggccga atggtgcggg ccctgcaaga tgatcgcgcc gatcctggac gaggtcgcgc
     7321 aggagtacgc cgggcgcctg aagatcgcga agctcaacat cgacgagaac cagggtacgc
     7381 ccccgaagtt cggcatccgc ggcatcccca ccctgatgct gttcaagaac ggcaacatgg
     7441 aggcgatgaa agtcggcgcc ctgtccaagt cccagctcac cgcgtttatt gacagcaata
     7501 tctaaagtct ttacgctccc cggtcgatat cccgtagaag tcaggcggcc ccgcccggca
     7561 ctgaagcgaa tgggcggcgc tgaaccagtg ggaagcgccg cggcgcgtga tgagcgccgg
     7621 cagagggagc accggccact ggggcccggc aagcccccag gcgccggatt catctccgcc
     7681 ttcccccttt cttctttccg tcccttcccc gtgtctgcac tgcccaaggt ccctccctcc
     7741 atgcatctgt ccgacctcaa gaacctccac gtcaccgaac tggtggagat ggccaatgcc
     7801 aacgagatcg agaacgccaa ccgcctgcgc aagcaggacc tcatcttcgc gctgctgaag
     7861 aaccaggcga agaagggcga gagcatcttc ggcgaaggca cgctagaggt gctgcccgac
     7921 ggcttcgggt tcctgcgttc cccggacacc tcgtacctgg ccggccccga cgacatctac
     7981 gtcagcccct cgcagatccg ccgcttcaac ctgcacaccg gcgattccat cgagggcgag
     8041 atccgcaccc cgaaggacgg cgagcgctac ttcgcgctgg tcaaggtgga caaggtcaac
     8101 tccgagccgc cggagaacgc caagaacaag atcctgttcg agaacctcac tccgctgcat
     8161 cccaccaacg tcatgatcct cgagcgcgac atccgcgccg aggagaacct gaccggccgc
     8221 atcatcgaca tcgtggcgcc ctgcggcaag gggcagcgcg gcctgatcgt gtcgccgccc
     8281 aaggccggca agacggtgat gctgcagcac atcgcgcacg cgatcacgtc gaaccatccc
     8341 gacgtcatgc tgatcgtgct gctgatcgac gagcgtcccg aggaggtcac cgagatgcag
     8401 cgctcggtgc gcggagaagt ggtgtcctcg actttcgacg agccggccac acgccacgtg
     8461 caggtggc
//
LOCUS       DASPGE010000283         9609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_310899, whole genome shotgun sequence.
ACCESSION   DASPGE010000283 DASPGE010000000
VERSION     DASPGE010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9609
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310899"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VD791_09090"
     CDS             complement(<1..321)
                     /locus_tag="VD791_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HXV08173.1"
                     /translation="MAAKPVYKRILLKLSGEALMGESPYGIDRATLERIVSEVAEVVR
                     LGVEIGIVIGGGNIFRGVALGAAGMDRATADYMGMLATVMNALALQDAMRQVGLLSRV
                     QSALN"
     gene            complement(336..1250)
                     /gene="tsf"
                     /locus_tag="VD791_09095"
     CDS             complement(336..1250)
                     /gene="tsf"
                     /locus_tag="VD791_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HXV08174.1"
                     /translation="MAQVTAGMVKELREKTDAPMMECKKALTEAGGDMAKAEEILRVR
                     LGSKASKAASRVTAEGTVAVHIAPDAKLGAIIEVNCETDFVAKNEDFLAFAGNLAELV
                     VRQAPPDVAALSGLKLGPATVEEVRTALFGRIRENISIRRFQRVEAQGRLTSYVHGGA
                     KIGVLVDVVGGDETLARDVAMHIAASKPLALSRDEVPAELVRTEREIAQQKAAESGKP
                     ANIVDKIVDGSVQKFLKDVTLLGQPFVKDDKISVEQLLKSKGARVQGFVLYVVGQGIE
                     RKQTDFAAEVAAQAAQAGKPTAAAGPAR"
     gene            complement(1276..2022)
                     /gene="rpsB"
                     /locus_tag="VD791_09100"
     CDS             complement(1276..2022)
                     /gene="rpsB"
                     /locus_tag="VD791_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HXV08175.1"
                     /translation="MAVSMREMLEAGVHFGHQTRYWNPRMAPFIFGQRNKIHIVNLEK
                     TLDLYNEALKFVRQLASNKGTLLFVGTKRQARDIVREEAARCGSPFVDYRWLGGMLTN
                     YKTVKQSIKRLKEMEQMVQDGTMERLAKKEQLHYQREMEKLNRSLGGIKDMNGLPDAL
                     FVIDVGYQKIAVAEARKLGIPVIAVVDTNNSPEGISYVIPGNDDSSRAVRLYARGVAD
                     AVLEGRSQVIKEIVTDEFVEVEGGEEAAAR"
     gene            complement(2234..2983)
                     /gene="rlmB"
                     /locus_tag="VD791_09105"
     CDS             complement(2234..2983)
                     /gene="rlmB"
                     /locus_tag="VD791_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HXV08176.1"
                     /translation="MRLLVGFHPVTARLRHDAGSVREILVDAGRRDPRLRDLIALAEQ
                     RGVRVLQVDEKRLDGLAAGARHQGVVARVGQIARPVDLDDLLQGLDQPPFLLLLDGVT
                     DPHNLGACLRTADAMGVHGVIVPKDRSAGLTATVAKVASGAAESVPLIAVTNLARAME
                     QLKEAGVWLLGAAEQGEQSLFEARLEGAVGWVMGAEGEGLRRLTRERCDALVRIPMFG
                     RVESLNVSVAAALCLFETRRQREGEKRDRKA"
     gene            complement(2988..5168)
                     /gene="rnr"
                     /locus_tag="VD791_09110"
     CDS             complement(2988..5168)
                     /gene="rnr"
                     /locus_tag="VD791_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HXV08177.1"
                     /translation="MKTTRERSVRSRKRVSLRRSDPHLERERARYENPLPSREWVLAV
                     LEREGVPVPQQRLETLLDIAPAEQEAFDRRLNAMERAGQIMRNRRGAICLAAKLDLIR
                     GRVHGHPDGYGFLIPDDGSPDLFLNPSEMSRVLHGDRALVRESGVDRRGRREGSIVEV
                     LERAQRRVVGRLHEEHGIALLVPEDKRISQDILVPREALAGAQTGQVAVVEIIQQPDH
                     HIKPVGRVVEVLGGYADPGMEVEIALRKHDLPYEFSPAVRSAADKLPHAVRKQDLDGR
                     RDLRALPLVTIDGETAKDFDDAVFCRPEGKGFRLWVAIADVSHYVAHGSALDLDARGR
                     GNSVYFPRRVIPMLPEELSNGLCSLNPDVDRLCVACEMAMGPRGDIKQYQFYPAVMHS
                     QARLTYTAVAAALEDDPGAVALGLVPHLHDLHRLFKVLAKARKQRGAIDFETVETRMD
                     FDEQGKIHRILPVERNDAHRLIEECMLAANVCASDILQAHEHPALYRVHEGPTPEKLA
                     DLREFLREFGLQLGGGDEPHAKDYARLLGQIRGRPDTQLLQTVLLRSLQQAVYSPENV
                     GHFGLAYEAYTHFTSPIRRYPDLLVHRAIKAVLAGESYRPGDWRELGVHCSQTERRAD
                     EATRDVEAWLKCYYMRERINETFNATISGVAGFGVFVALDEVYVEGMVHVSELGNDYY
                     HFDASRHQLMGERTRRRFRLGDRVRVRLVRADLETTRLDFVLAG"
     gene            5238..5324
                     /locus_tag="VD791_09115"
     tRNA            5238..5324
                     /locus_tag="VD791_09115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5272..5274,aa:Leu,seq:cag)
     gene            complement(5453..6748)
                     /locus_tag="VD791_09120"
     CDS             complement(5453..6748)
                     /locus_tag="VD791_09120"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HXV08178.1"
                     /translation="MGRNVVVVGTQWGDEGKGKVVDWLTEHAQAVVRFQGGHNAGHTL
                     VIGGRKTVLHLIPSGILRQGVACFIGNGVVVSPQALLEECATLEQAGVDVRARLTISE
                     ACPLILPYHTALDRAREAAKGAGKIGTTGRGIGPAYEDKVARRAIRIQDLFHRERFAA
                     KLGEVLDYHNFVLRQYFGVEPVDFQATLEEALALADRIKSMIGDVPRRLSEAQRAGHN
                     LLFEGAQGTLLDVDHGTYPFVTSSNCVAGAAAAGSGVGPSALHYVLGITKAYTTRVGS
                     GPFPTELEDDIGRQMATRGNEVGATTGRPRRCGWFDAAALKRSIQINGASGLCVTKLD
                     VLDGMETLQIGIGYRVAGQMVDLLPVGAEELAECEPIYEEIPGWTQTTLGVRRYQDLP
                     KQARAYLQRIAEVCEVPLDMISTGAERDDTIVLRHPFAA"
     gene            complement(6766..7932)
                     /locus_tag="VD791_09125"
     CDS             complement(6766..7932)
                     /locus_tag="VD791_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HXV08179.1"
                     /translation="MRRWLLPEYIEDILPAEAQRIEALRRRILDLFRVHGYELVMPPL
                     LEYLESLLTGTGHDLDLKTFKLVDQLSGRLLGVRADITPQTARIDAHLLNRAGIARLC
                     YAGSVLHALPSGMMRTREPLQIGAELYGHAGVESDVEIQGLMVGALHAAGVGEPRVDL
                     GHVGVFRAMMTRFRVPAELESELFQVLQIKDVPALRELARGLDAQLAAALLLLPQLYG
                     GAQTLEEARRRLPAWPEIGTAIDQLEIIESALHRAGQTVHCFDLAELRGYHYHSAVVF
                     AAYASGRPNAIAQGGRYDEVGKAFGRARPATGFTMDLRELAALGGRPAAARAVLAPYR
                     PGEGALQRKVEELRAGGEVVIVDLPGHERTRAELGCDRELVLRDGAWEVVAASF"
     gene            complement(7945..8142)
                     /locus_tag="VD791_09130"
     CDS             complement(7945..8142)
                     /locus_tag="VD791_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HXV08180.1"
                     /translation="MGSSVWMAVALMLVLEGALPFLMPRVWRDTFRRLIEMTDGQIRF
                     VGLASMLIGLVLLLVLTSLRG"
     gene            complement(8188..9063)
                     /gene="hflC"
                     /locus_tag="VD791_09135"
     CDS             complement(8188..9063)
                     /gene="hflC"
                     /locus_tag="VD791_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HXV08181.1"
                     /translation="MNRNLTLLLVGLVAALIVASLTLFTVDQRQHAIVFRLGEPVNVI
                     RSPGLYLKVPLLDNVRFFDVRILTLDADEPERFITSEKKNVLVDSFVKFRIVDVLQYY
                     ISVGGDEARAKIRLSQTMNDSLRAEFGKRTIHEVVSGERDRIMELMRAKADADARTIG
                     VEVLDVRLKRVDLPQEVGESVYRRMEAERKRVANELRSLGFAEAEKIRADAERQRSII
                     IAEAYRDAQRVKGEGDARSAAIYARAFQQNPEFFAFYRSMEAYRHSFKSRSDVMVIDP
                     SSDFFRYLEKPAGRR"
     gene            complement(9060..>9609)
                     /gene="hflK"
                     /locus_tag="VD791_09140"
     CDS             complement(9060..>9609)
                     /gene="hflK"
                     /locus_tag="VD791_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HXV08182.1"
                     /translation="LDRYRTGINISRVTMQQAQPPEQVQAAFDDAVKAGQDRERQINE
                     GQAYANDVVPKARGTASRLMQEAEGYKQSVIANAEGEASRFRQVQNEYARAPAVTRER
                     LYLETMQQVYGNATKVYVDQRGGNNILFLPLDKIIQMSASGAVPALEGTAPGVEPPAN
                     APTGADPRSRDAFRSRDREARP"
BASE COUNT         1398 a         3473 c         3153 g         1585 t
ORIGIN      
        1 gttcagcgcc gactgcaccc ggctcagcag cccgacctgg cgcatggcgt cctgcagcgc
       61 cagggcgttc atgaccgtgg ccagcatgcc catgtaatcg gccgtcgcac gatccatgcc
      121 ggcggctccc aaagccacgc ctcggaagat gttgcccccg ccgatcacga tgccgatttc
      181 cacgcccagc cgcaccacct cggcgacctc ggagacgatg cgctccagcg tggcgcggtc
      241 gatgccgtag gggctctcgc ccatcagggc ctcgcccgag agcttgagca ggatgcgctt
      301 gtagaccggc ttcgccgcca cgggccgggg gctcctcagc gtgcgggccc ggcagccgcg
      361 gtcggctttc ccgcctgggc cgcctgggcc gccacctcgg cggcgaaatc ggtctgcttc
      421 ctctcgatcc cctggcccac cacgtacagc acgaagccct gcacccgcgc gcccttggac
      481 ttgagcagct gctccacgct tatcttgtcg tccttcacga acggctgccc gagcagagtg
      541 acgtccttga ggaatttctg cacgctgccg tccacgatct tgtcgacgat gttggccggc
      601 ttgcccgatt cggccgcctt ctgctgggcg atctcgcgct ccgtccggac cagctcggcc
      661 ggaacctcgt cgcgcgacag cgccagcggc ttgctggcgg cgatgtgcat agccacgtcc
      721 ctggccaggg tctcgtcccc gccgaccacg tccaccagca cgccgatctt ggccccgccg
      781 tgcacgtagc tggtcagccg cccctgggcc tcgacccgct ggaagcggcg gatcgagatg
      841 ttctcgcgga tgcgcccgaa cagagcggtg cggacctcct cgaccgttgc cggccccagc
      901 ttcagcccgg acagcgcggc gacgtcgggc ggtgcttgcc gcaccaccag ctcggccagg
      961 ttgcccgcga aggccaggaa atcctcgttc ttggccacga agtcggtctc gcagttgacc
     1021 tcgatgatcg cgcccagctt cgcgtccggc gcgatgtgca cggccaccgt gccctctgcc
     1081 gtcacgcggc tcgccgcctt gctggccttg ctgcccagcc gcacccgcag gatctcctcg
     1141 gccttggcca tgtcgccgcc ggcctcggtc agcgccttct tgcactccat catcggcgcg
     1201 tcggtcttct cgcgcagctc cttgaccatg ccagcggtga cttgcgccat gctcgcctct
     1261 cccgttcacc ggcgctcagc gcgccgccgc ttcctcgccg ccctcgacct cgacgaactc
     1321 gtcggtgacg atctccttga tcacctgcga gcggccttcc aggacggcgt cggccacgcc
     1381 gcgggcgtac aggcgcaccg cgcggctcga gtcgtcgttg ccggggatca cgtagctgat
     1441 gccctcgggc gagttgttgg tgtcgaccac cgcgatcacc gggatgccca gcttcctcgc
     1501 ttccgccacg gcgatcttct ggtagcccac gtcgatcacg aacagcgcgt cgggcaggcc
     1561 gttcatgtcc ttgatgccgc ccaggctgcg gttgagtttc tccatctcgc gctggtagtg
     1621 cagctgctcc ttcttcgcca gccgctccat ggtgccgtcc tggaccatct gctccatctc
     1681 cttgaggcgc ttgatggact gcttgaccgt cttgtagttg gtcagcatgc cgcccagcca
     1741 acggtaatcc acgaacggcg agccgcagcg ggcggcttcc tcgcgcacga tgtcgcgcgc
     1801 ctggcgcttg gtaccgacga acagcaacgt gcccttgttg gacgcgagct ggcgcacgaa
     1861 cttgagcgcc tcgttgtaca ggtcgagggt cttctcgagg ttgacgatgt ggatcttgtt
     1921 gcgctggccg aagatgaacg gcgccatcct cgggttccag tagcgggtct gatgtccgaa
     1981 atggacgccc gcctccagca tttcgcgcat ggatacagcc atgaatcgat tctccggtta
     2041 aggttgagcc gccatccgcc cgggcgacgt ccttcgacgc caccgcaaca cccgcctcgc
     2101 ggcgggcacc ttaactcagt gcggatgtgt gggatgttcc gccgccggcc ccggccggtc
     2161 gagcgaattc gaaagtatat cagggagatg gggtggggat ccaggagtga ccgcagggcg
     2221 tggccgggaa gcgtcacgcc ttccggtcac gcttttcgcc ctcccgctgg cggcgcgtct
     2281 cgaacaggca cagggccgcc gcgaccgaca cgttcaggct ttccacccgt ccgaacatgg
     2341 gaatacgcac cagcgcgtcg catcgctcgc gggtcagccg gcgcaggccc tcgccttcgg
     2401 cccccatcac ccagcccacg gcgccttcca gtcgcgcctc gaacaagctc tgctccccct
     2461 gctccgccgc gccgagcagc cacacgcccg cctccttgag ctgctccatg gcgcgcgcta
     2521 ggttggtcac cgcaatcagc ggcacgctct cggccgcgcc gctggccacc ttggccacgg
     2581 tggccgtcag gcccgcggaa cggtccttgg gcacgatcac cccgtgcacg cccatcgcgt
     2641 cggcggtgcg caggcaagcg ccgaggttgt gcgggtcggt gacgccgtcc agcagcagca
     2701 gaaacggcgg ctgatcgagc ccctggagca ggtcgtccag atccaccgga cgcgcgatct
     2761 ggcccacgcg ggccacgacg ccctggtgcc gtgcgccggc ggcgaggccg tccaggcgct
     2821 tttcgtccac ctgcagcacg cgcacgccac gctgctcggc cagcgcgatc aggtcgcgca
     2881 gacgcgggtc ccgccgcccg gcgtcgacga gaatctcgcg cacgctgccc gcgtcgtgcc
     2941 gcaggcgcgc ggtgacggga tggaagccaa ctagcaggcg catccgctca cccggccagc
     3001 acgaagtcca gacgagtggt ctccaggtcg gcgcgcacca ggcgcacccg gacacggtcg
     3061 cccagccgga agcggcgccg cgtgcgctcg cccatcagct ggtggcgcga agcgtcgaaa
     3121 tggtaataat cgttgcccag ctccgacacg tgcaccatgc cttcgacgta cacctcgtcc
     3181 agggcgacga acaccccgaa gccggcgacg ccgctgatgg tggcgttgaa agtctcgttg
     3241 atacgctcgc gcatgtagta gcacttgagc caggcttcga cgtcgcgcgt ggcctcgtcg
     3301 gcgcggcgct cggtctgcga gcagtgcacg cccagctccc tccagtcgcc cggccggtag
     3361 ctctcgccgg caagcaccgc cttgatggcc cggtgcacaa gcaggtcggg atagcgccgg
     3421 atcggcgagg tgaagtgcgt gtacgcctcg taggccagac cgaagtggcc cacgttctcg
     3481 gggctgtaga ccgcctgctg cagggagcgc agcaataccg tctgcagcag ctgcgtgtcg
     3541 gggcggcccc ggatctgccc cagcagcctg gcgtagtcct tcgcgtgcgg ttcgtcgccg
     3601 ccgcccagct gcagcccgaa ctcgcgcagg aactcgcgca ggtcggccag cttctccggt
     3661 gtgggcccct cgtggacgcg gtacagcgcc ggatgctcgt gcgcctgcag gatgtcggac
     3721 gcgcacacgt tggccgcgag catgcattcc tcgatcagcc ggtgcgcgtc gttgcgttcg
     3781 accgggagga tgcggtggat cttgccctgc tcgtcgaagt ccatgcgcgt ttcgacggtc
     3841 tcgaagtcga tggcgccgcg ctgcttgcgt gccttcgcca gcaccttgaa cagcctgtgc
     3901 agatcgtgca gatgcggcac aaggccgagc gccaccgcgc ccggatcgtc ttccagcgcc
     3961 gcggccaccg cggtataggt aagccgcgcc tgggaatgca tcacggcggg gtagaactga
     4021 tactgcttga tgtcgccgcg cggccccatc gccatctcgc aggcgacgca cagccggtcg
     4081 acgtccgggt tcagcgagca cagcccgttg gacagctcct cgggcagcat cggaatcacg
     4141 cgccgcggga agtacaccga gttgccgcgg ccgcgcgcgt cgagatcgag cgcgctgcca
     4201 tgcgcgacgt agtggctgac gtcggcgatg gccacccaca gccggaagcc cttgccttcc
     4261 ggccggcaga acacggcgtc gtcgaagtcc ttggcggttt cgccgtcgat ggtcaccagc
     4321 ggcagcgcac gcaggtcgcg gcgcccgtcc aagtcctgct tgcgcacggc gtgcggcaac
     4381 ttgtccgcgg cgctgcgcac cgcaggggag aactcgtagg gcagatcgtg cttgcgcagc
     4441 gcaatctcga cttccatgcc cgggtccgcg tagccaccca gcacctctac cacgcggccc
     4501 accggcttga tgtggtgatc gggctgctgg atgatttcca ccaccgccac ctggccggtt
     4561 tgcgcgccgg ccagcgcctc tcgcggcacc aggatgtcct ggctgatgcg cttgtcttcg
     4621 ggcaccagca gcgcgatccc gtgctcctcg tgcaggcggc cgaccacgcg ccgctgcgcg
     4681 cgctcgagca cttccacgat cgacccctcg cgccgcccgc gccggtccac gcccgactcg
     4741 cgcaccaggg cgcggtcgcc gtgcagcacg cgcgacatct ccgacgggtt gaggaacagg
     4801 tcggggctgc cgtcgtcggg gatcaggaag ccgtagccgt cgggatggcc gtgcacgcgg
     4861 ccgcgaatca agtcgagctt ggccgccagg cagatcgctc cgcgccggtt gcgcatgatc
     4921 tgcccggcgc gctccatcgc gttcaagcgc cggtcgaagg cctcctgctc cgccggcgcg
     4981 atgtcgagca gggtctccaa gcgctgctgc ggcacgggca cgccttcgcg ctcgagcacc
     5041 gccaggaccc actcgcggct gggcagcggg ttctcgtaac gcgcgcgctc gcgctcgaga
     5101 tgggggtcgg aacggcgcag gctgacgcgc ttgcgcgagc gcacgctacg ctcgcgggtg
     5161 gtcttcatcg acgcattcgc gccgctgcgc gctgatttga caagactggc attttcatta
     5221 gaatttacgc ccgctgtgcc gagatggcgg aattggtaga cgcactaggt tcaggtccta
     5281 gcgcccgcaa gggtgtggag gttcgagtcc tcttctcggc accagggttt tatgcgccgg
     5341 gtgcgcagga aagcgcggcc taatgcgcat taaagcgcat cttaaaaaga agaacgcccg
     5401 ttcgctcaga gcatcacctc gcgtggtact gtacgctcat ccatagagca cctcaagcgg
     5461 cgaacgggtg cctcaagacg atcgtgtcgt cgcgctcggc gccggtggag atcatgtcga
     5521 gcggcacctc gcacacttcg gcgatgcgct gcaggtaggc gcgcgcctgc ttcggcaagt
     5581 cctggtagcg gcgcacgccc agggtggtct gcgtccagcc cggtatctcc tcgtagatcg
     5641 gctcgcactc ggccagctct tcggcaccta ccggcaacag atcgaccatc tgaccggcca
     5701 cgcggtagcc gatgccgatc tgcagcgtct ccatgccgtc cagcacgtcc agcttggtga
     5761 cgcacaggcc gctcgcgccg ttgatctgga tcgagcgctt cagcgcggcc gcgtcgaacc
     5821 agccgcagcg ccgcgggcgc ccggtggtgg cgccgacctc gttgccgcgt gtggccatct
     5881 ggcgcccgat gtcgtcctcg agctcggtgg gaaacgggcc ggaacccacg cgcgtggtgt
     5941 aggccttggt gatccccagc acgtagtgca gcgcgctggg ccccactccg ctgccggccg
     6001 ccgcggcccc ggccacgcag ttgctggagg tgacgaacgg ataggtgccg tggtccacgt
     6061 ccagcagcgt gccctgggcg ccctcgaaca gcagattgtg gccggcgcgc tgcgcctcgg
     6121 acagccggcg tggcacgtcg ccgatcatgc tcttgatgcg gtccgccagc gccagcgcct
     6181 cttccagcgt ggcctgaaag tccacgggct cgacgccgaa gtactggcgc agcacgaagt
     6241 tgtggtagtc gagcacctcg cccagcttcg cggcaaagcg ctcgcggtgg aacagatcct
     6301 ggatgcggat cgcgcggcgc gccaccttgt cctcgtaggc ggggccgatg ccgcggccgg
     6361 tggtgccgat cttgccggcg cccttggccg cctcgcgcgc gcggtcgagc gcggtgtgat
     6421 acggcaggat caacgggcag gcttcgctga tggtgagccg cgcgcgcacg tccacgccgg
     6481 cctgctcgag cgtggcgcac tcctcgagca gcgcctgcgg cgacaccacc acgccgttgc
     6541 cgatgaagca ggccacgccc tggcgcagga taccggaggg aatcaggtgc agcaccgtct
     6601 tgcggccgcc gatcaccagc gtgtgcccgg cgttgtgacc gccctggaag cgcaccaccg
     6661 cctgggcgtg ctcggtgagc cagtccacca ccttgccctt gccttcgtcg ccccactgcg
     6721 tgcccaccac cactacgttg cgtcccatcg ccgccgcctc aacggttaaa agctcgccgc
     6781 cacgacttcc caagcgccgt cgcgcaacac cagctcgcga tcgcagccca gctcggcgcg
     6841 cgtgcgctcg tgccccggca ggtccacgat caccacctct ccgcccgcgc gcagctcctc
     6901 gaccttgcgc tgcaacgctc cctccccggg ccgatacggc gccagcacgg cgcgcgccgc
     6961 cgccggccgg ccgcccaacg cggccagctc gcgcagatcc atggtgaacc cggtggccgg
     7021 ccgcgcgcgt ccgaacgcct tgcccacctc gtcgtagcgg ccgccctgcg cgatggcgtt
     7081 gggacgtccg ctcgcgtatg ccgcgaacac gactgcgctg tgatagtgat agccgcgcag
     7141 ctcggccaga tcgaagcagt gcaccgtctg ccccgcgcga tggagcgcgc tctcgatgat
     7201 ctcgagctgg tcgatggcgg tgccgatctc gggccaggcc ggcagccgcc gtcgcgcctc
     7261 ctccagcgtc tgcgccccgc cgtagagctg cggcagcagc agcagcgctg cggcgagctg
     7321 cgcatcgagc ccgcgagcca gctcgcgcaa cgcgggcacg tccttgatct gcagcacctg
     7381 gaacagctcc gactcgagct cggccggcac gcgaaagcgc gtcatcatgg cgcggaacac
     7441 gccgacgtgg ccgagatcca cgcgcggctc gccgacaccc gccgcatgca gggcgccgac
     7501 catcagaccc tgaatctcga cgtcgctctc cacaccggcg tggccgtaca gctccgcccc
     7561 gatttgcagc ggctcgcgcg tgcgcatcat gccggagggc agcgcgtgca ggacgctgcc
     7621 cgcgtagcac agccgcgcga tgccggcacg gttgagcagg tgcgcgtcga tgcgcgccgt
     7681 ctgcggcgtg atgtcggcgc gcacgcccag caggcgaccg gacagctgat ccaccagctt
     7741 gaaggtcttc aagtcgaggt cgtgcccggt gccggtgagc agcgactcca ggtactcgag
     7801 cagcggcggc atcaccagct cgtagccgtg cacccggaac aggtccagga tgcgccggcg
     7861 cagcgcctcg atgcgctgcg cctcggccgg caggatgtct tcgatgtact cgggaaggag
     7921 ccagcggcgc atgatcgggc gggttcagcc tctgagactc gtcaacacca gcagcaggac
     7981 caggccgatc agcatcgagg cgagtcccac gaagcggatc tggccgtcgg tcatctcgat
     8041 caggcgccgg aaggtgtcgc gccacacgcg cggcatcagg aacggcaatg cgccttccag
     8101 caccagcatc agcgccacgg ccatccacac gctgctgccc aaggcgctcg cctccgttcc
     8161 ttcgcgaccc ggccgggcga ctgcgcgcta gcggcggccg gcgggtttct ccagatacct
     8221 gaagaaatcc gagctcgggt cgatcaccat cacatcgctg cggctcttga agctgtgccg
     8281 gtaggcctcc atgctgcggt agaaggcgaa gaactccggg ttctgctgaa aggcgcgcgc
     8341 gtagatcgcc gcggagcggg cgtcgccctc gcccttcacg cgctgggcgt cgcggtaggc
     8401 ctcggcgatg atgatcgagc gctgccgttc ggcatccgcg cggatcttct cggcctcggc
     8461 gaaacccagc gagcgcagct cgttggccac gcgcttgcgc tcggcctcca tgcggcggta
     8521 caccgactcg cccacctcct gcggcaggtc cacgcgcttc agacgtacgt cgagcacctc
     8581 cacgccgatg gtgcgtgcgt cggcgtcggc tttggcgcgc atcagctcca tgatgcggtc
     8641 gcgctccccg gacaccactt cgtggatggt gcgcttgccg aactccgcgc gcaggctgtc
     8701 gttcatggtc tgcgacagac ggatcttggc acgcgcctcg tcgccgccca cgctgatgta
     8761 gtactgcagc acgtcgacga tgcggaactt gacgaaggag tccaccagga cgttcttctt
     8821 ctccgaggtg atgaagcgtt ccggctcgtc cgcgtccagg gtgaggatgc gcacgtcgaa
     8881 gaagcgcacg ttgtcgagca gcggcacctt caggtacagg cccgggctgc ggatcacgtt
     8941 caccggctcg cccaggcgga acacgatggc gtgctggcgc tggtcgacgg tgaacagcgt
     9001 gagactggcg acgatcagcg ccgccaccag ccccaccagc agcaaggtca ggttgcggtt
     9061 catggccggg cctcccggtc gcggctgcgg aacgcgtcgc gcgagcgcgg gtcggctccc
     9121 gtcggcgcgt tcgccggcgg ctcgacgccc ggagccgtgc cctccagcgc cggcaccgcg
     9181 ccgctcgcgc tcatctggat gatcttgtcg agcggcagga acaggatgtt gttgcccccc
     9241 ctctgatcca cgtacacctt ggtggcgttc ccgtacacct gctgcatggt ctccaggtac
     9301 aggcgctcgc gcgtcacggc gggcgcgcgc gcgtactcgt tctgcacctg acggaagcgc
     9361 gaggcttcgc cttcggcgtt ggcgatgacg ctctgcttgt agccttcggc ttcctgcatc
     9421 aggcgcgaag ccgtgccgcg cgccttgggc accacgtcgt tggcgtaggc ctggccctcg
     9481 ttgatctggc gctcgcggtc ctggcccgct ttgaccgcgt cgtcgaaggc ggcctgcacc
     9541 tgctccggcg gctgcgcctg ctgcatggtg acgcggctga tgttgatgcc cgtccggtag
     9601 cggtcgagg
//
LOCUS       DASPGE010000284         6521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_543484, whole genome shotgun sequence.
ACCESSION   DASPGE010000284 DASPGE010000000
VERSION     DASPGE010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543484"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1898
                     /locus_tag="VD791_09145"
     CDS             <1..1898
                     /locus_tag="VD791_09145"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HXV08183.1"
                     /translation="LTLDIVSMLFDEIFDDARVAVAMKGLIGRLQIPMLKVALLDKNF
                     FSDKAHPARRLLDALGEIGLGLPEDFDHTDALYKQIEAVLQELIDGFREEMQIFDDSR
                     EQLERLIAEQNRDAEQEAARVAQGIEQKEKLELAKAVAQDEIKRRAAERKIPRVVLKF
                     LAEQWVKLLLVAHAKHGADSELWKAALATMDSLIWSVTPKPSVDERRALGDKLPGLLK
                     RLSAGMRMIRVDDDTRKQFFAKLMRCHTNIINGVDGSAAKPAATAQPTTDAKPELAPA
                     QPAASAKPQPAPAQPAASAKPQPAPAQPAASAKPQPAPAQPAPSAKPKPAPAQPAVSA
                     KPQPALTQAQPTPSVQKAPAASPATAAQAVSPNQTAQAAPSAQSPQAAPARAQQAPLA
                     AQAGTPAKSPTLPSTPSARATPVASTQAARPAQSPPKTPAQSAAPAQPTPSAPSGTPA
                     PEPSGLPEDDSLDFTGLGSDPTAPLPPAAARRQGGAPDEDTEPAAAPPSFSPVKIDNP
                     FGEGEIEVEEINLSELPGAAGRKAAGKLVHDEHTRLVASLKEGAWVELRIQEQQWQPA
                     RLSYISPVKGIFLFVNRQGRKVGEYSLVQLAREFRSGRAAVLDSVPLFDRAMTSLVGV
                     LKGGVGK"
     gene            complement(1916..3400)
                     /locus_tag="VD791_09150"
     CDS             complement(1916..3400)
                     /locus_tag="VD791_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXV08184.1"
                     /translation="MRDEQELTLLIKSRFPLVLIETHEEARALALLEKICNLQSLPLF
                     VWSVTAGLRRQGRNESVPQTGDPAQALRHIEKTLQNGVYALLDFHPFLEDALHQRLTR
                     EIAMGYHKTARTLVFVSPQLELNNTLTRMSARFRLSVLDVAAVKQMIRDEADLWKAER
                     GGERLRGQKEAVDLLAQHLAGMSADDARRLVRQAIRDDTCLNLEDVSRVLKHKHQSLA
                     ADSLLALELDTTGFAEVGGLGNLKRWLERRRAVFTGAAQAQGLDAPKGILLLGVQGGG
                     KSLAAKAVAGAWGVPLLSLDFGALYNKFFGQTERNLREALAAAEAMAPCVLWMDELEK
                     GVGGDATGDSDGGVSRRVLGTLLTWMAERKSRVFLVATANDIARLPPELIRKGRFDEI
                     FFVDLPDAATRKEIFAIHLRRRKALPESFDLDALARAADGFSGAEIEQVIVSGLYEAH
                     ASGGSLDTEILKREIAGTRPLSTTMAEHIQALRDWAQERTVPAN"
     gene            3506..4420
                     /gene="rsgA"
                     /locus_tag="VD791_09155"
     CDS             3506..4420
                     /gene="rsgA"
                     /locus_tag="VD791_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HXV08185.1"
                     /translation="MLSLADRPRSLLSGQVVAVHGRHYLVELADGRTIGCFPRGKKSL
                     VACGDRVGVLPTAKDQGVIEAVDPRRSLLYRSDRHREKLIAANVTQMIVVLAAVPSFY
                     EELLCRCLAAAEYQEVRALIVLNKFDLVEQSARAVAALELYRALGYEVLPLIAKHEVT
                     PLRAHLHGHTSVLVGQSGMGKSTIVNALVPGAQAPVSEISAALDSGRHTTTHARLYRL
                     DAHSALIDSPGLQEFGLAHVPVRELARTFVEFRPLLGQCRFGDCLHRGEPDCTLAAAA
                     DRGQVSERRLQIYRRLLEEAMRPPSARG"
     gene            complement(4392..5318)
                     /locus_tag="VD791_09160"
     CDS             complement(4392..5318)
                     /locus_tag="VD791_09160"
                     /inference="COORDINATES: protein motif:HMM:NF005792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HXV08186.1"
                     /translation="MSFFTIIAALLIERTRPLPWGSALLAAFRGYADRLAHDLNAGKV
                     SHGTVGWFAAVCPWVLAALMVFYLLHWLNPVLGWVWTVAVLYTCIALRELVQALKGIF
                     DALRAGDLERARSLLLQWRGEPAGAYGEGEIAKAAIETALLRAHREVFAVIFWFVLLP
                     GPAGALLYRLAAEMEQRWGRRRDDEFRAFGRFSARAFRVLDWLPVRLSAVGFAIAGNF
                     EDAIAAWRSYAQSWIDPAQGILLASGAGALGVRLGGPLPLESGVDFRPDLGEGATPDA
                     EYLQSALYLLWRTLIVWLGLLLLLTLARWAGA"
     gene            complement(5417..>6521)
                     /locus_tag="VD791_09165"
     CDS             complement(5417..>6521)
                     /locus_tag="VD791_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXV08187.1"
                     /translation="DDNLFLWDLGDAGEHREDRAVTYEAVAMSLEDAGEQTELEHAGG
                     EQVQPDAVPSDAAAAEGAASVEEAPAQQYLYDEWDYLIGAERRRWCTLLEKLPPAGDP
                     RAIDEVLRRNEETVQRLTTLIKAVQIQRPRRLRKQLEGDRLDLDACIAATLDLRSGRT
                     PDPRVHQRLGRNNRDLAVLLLLDLSQSTNDWVPGAGSTVLNLAREATALVAHAMHDLG
                     DSFAIHGFDSNGRHGVEYYRFKDFDEPYGEQARARLAGMRGQLSTRMGCALRHAGHFL
                     RGRRVAQRLVLLLTDGEPHDIDVLDKQYLVLDTKRAVEDQRRYGIATYCISLDPAADE
                     YVVRIFGARNYMVLDQLRRLPETLPALYLRLTT"
BASE COUNT         1041 a         2328 c         2219 g          933 t
ORIGIN      
        1 cgttgacgct ggacatcgtg tccatgctgt tcgacgagat cttcgacgac gcgcgcgttg
       61 cggtggccat gaaaggcctc atcgggcggc tgcagatccc gatgctgaag gtagcgctgc
      121 tggacaagaa cttcttctcc gacaaggcgc atcccgcgcg caggctgctc gacgcactgg
      181 gcgagatcgg cctgggtctg cccgaggatt tcgatcacac cgacgcgctc tacaagcaga
      241 tcgaggcggt gctgcaagag ctgatcgacg gcttccgcga ggagatgcag atcttcgacg
      301 actcgcgcga gcagctcgag cgcctgatcg ccgagcagaa tcgggacgcg gagcaggaag
      361 ccgcgcgcgt ggctcagggc atcgagcaga aggagaagct ggagctcgcc aaagcggtgg
      421 cccaggacga gatcaagcgg cgcgcggcgg agcgcaagat cccgcgcgtg gtgctgaaat
      481 tcctggccga acagtgggtc aagctgctgc tggtggccca cgccaagcac ggtgccgaca
      541 gcgagctgtg gaaagccgct ctcgccacca tggattcgct gatctggagc gtgaccccga
      601 agccgtctgt ggacgagcgg cgggcgctgg gcgacaagct gcccgggttg ctcaagcggt
      661 tgagtgcggg catgcgcatg atccgggtgg acgacgacac ccgcaagcag ttcttcgcca
      721 agctcatgcg ctgccatacc aacatcatca acggcgtgga cggatctgca gcgaagcctg
      781 ccgcgaccgc gcagcccacg accgacgcca aacccgagct ggccccggca cagccggcgg
      841 ccagcgccaa gccgcaaccc gctccggcac agccggcggc tagcgccaag ccccagcctg
      901 ccccggcaca gccagcagcc agcgccaagc ctcagcccgc cccggcgcag ccagcgccca
      961 gtgccaagcc caaaccggcg ccggcgcagc cagcggtgag cgccaagccg cagcctgccc
     1021 tgacacaagc gcagccgacg ccatcggtgc agaaggcgcc ggcggcatcg ccggcgaccg
     1081 cggcgcaggc ggtctcaccg aaccagacgg cacaggcggc accgtccgca cagagcccac
     1141 aggcggcgcc ggcgcgggct cagcaggctc cgctggcggc acaagcgggg acacccgcga
     1201 aatcgcccac cctaccatcg acaccgtccg cgcgcgccac gccggtggca tcgacacagg
     1261 cggcacggcc cgcgcagtcg cccccgaaaa caccggcaca gtcggccgct ccggcacagc
     1321 cgacgccatc ggcgccttcg ggcacgccag cgccggagcc gtccgggctc cccgaggacg
     1381 actcgctcga cttcaccggg ctcggttcgg accccaccgc accgctgccc ccggctgcgg
     1441 cgcgacgcca gggcggcgcg ccggacgagg acaccgagcc ggcggcagca ccgccgagct
     1501 tctcaccggt gaagatcgac aacccgttcg gagagggcga gatcgaggtc gaggagatca
     1561 acctttcgga gctgcccggc gcggcgggac gcaaggccgc cggcaagctg gtgcacgacg
     1621 agcacacgcg cctggtggcc agcctgaagg agggcgcctg ggtcgagctg cgcatccaag
     1681 aacagcaatg gcagccggcg cgcctgtcct acatcagccc ggtcaagggc atcttcctgt
     1741 tcgtcaaccg ccaggggcgc aaagtcggcg aatactccct ggtccagctc gcgcgcgagt
     1801 tccgcagtgg ccgcgcggcg gtcctcgaca gcgttccgct gttcgaccgt gcgatgacca
     1861 gcctggtggg cgtgctcaag ggcggcgtgg gaaagtagcc cgacatcaag ccgcgctaat
     1921 tcgctggcac cgtgcgctcc tgcgcccagt cgcgcagcgc ctggatatgc tccgccatcg
     1981 tggtcgacag cggccgggtg ccggcgatct cgcgcttcag gatctccgtg tcgagcgacc
     2041 cgccgctggc gtgagcctcg tacagccccg acacgatcac ttgctcgatc tcggcgccgg
     2101 agaacccgtc ggccgcccgc gccagtgcgt cgagatcgaa ggactccggt aacgccttgc
     2161 gccgccgcag gtggatggcg aagatctcct tgcgagtggc cgcatccggc aggtccacaa
     2221 agaagatctc gtcgaagcgc cccttgcgga tcagctcggg gggcaggcgc gcgatgtcgt
     2281 tggcggtggc gaccaggaac acgcggctct tgcgttccgc catccaggtg agcagggtac
     2341 ccagaacgcg ccgtgacacg ccgccgtcgc tgtccccggt agcgtcgccg cccactccct
     2401 tctccagctc gtccatccac agcacgcacg gcgccatcgc ttccgcggcg gccagcgctt
     2461 cacgcaagtt gcgctcggtc tgcccgaaga acttgttgta cagagcgccg aaatccaggc
     2521 tcagcagcgg cacgccccag gcgcccgcca ccgctttcgc cgcgaggctc ttgccgccac
     2581 cctgaacgcc cagcagcagg atgcctttgg gcgcatccag accctgcgcc tgggcggcgc
     2641 cggtgaagac ggcgcgccgc cgctccagcc agcgcttgag gttgcctagg ccgccgacct
     2701 cggcgaagcc ggtggtgtcg agctccaggg ccagcaggct gtccgcggcc agcgactggt
     2761 gcttgtgctt gagcacgcgc gacacatctt ccaggttgag acaggtgtcg tcgcggatcg
     2821 cctggcgcac cagccggcgg gcatcgtcgg cgctcatccc cgcgaggtgc tgcgccagca
     2881 gatcaaccgc ctccttctgg ccgcgcaggc gctcgccgcc gcgctccgcc ttccacaggt
     2941 cggcctcgtc gcggatcatc tgctttacgg cggcgacatc cagcaccgac agccggaagc
     3001 gggcgctcat gcgcgtgagg gtgttgttga gttcgagctg cggactgacg aagaccagcg
     3061 tgcgcgctgt cttgtggtag cccatcgcga tttcgcgggt caggcgctgg tgcagtgcgt
     3121 cctcgaggaa cgggtggaag tcgagcagcg cgtacacgcc gttctgcagc gtcttctcga
     3181 tgtggcgcag cgcctgggcc ggatcgccgg tctgcggcac cgactcgttg cgcccctgcc
     3241 ggcgcagccc ggcggtgacc gaccagacga aaagcggcag ggactgcagg ttgcagatct
     3301 tctccagcag agcaagcgcg cgcgcctcct cgtgcgtctc gatgagcacc agcgggaagc
     3361 gggacttgat gaggagcgtg agttcctgct cgtcgcgcat gtcggccggt tcatgcaggc
     3421 ttcatgccca gtacgccacc agggcgctcg cggcgccgca gcctctcgcg cgccgggccg
     3481 cgttaaaatg accgcgtgcg cggggatgct ctcattggct gaccggccgc ggtccctcct
     3541 gtccggccag gtggtcgccg tgcacggtcg ccattacctg gtcgagctgg ccgacggccg
     3601 caccatcggc tgttttccac gcggcaagaa aagcctggtc gcctgcggcg accgcgtcgg
     3661 cgtgctgccc accgccaagg accagggggt gatcgaggcg gtcgacccgc ggcgctccct
     3721 gctgtatcgc tccgatcgac atcgcgagaa gctgatcgcc gccaacgtca cgcagatgat
     3781 cgtggtgctg gcggcggtgc cctcgttcta cgaagagctg ctgtgccgct gcctggcggc
     3841 ggccgagtac caggaggtgc gcgcgctgat cgtgctgaac aagttcgatc tggtggagca
     3901 gagcgcgcgc gccgtcgccg cgctcgagct gtatcgtgcg ctgggctacg aagtgctgcc
     3961 gctgatcgcc aagcacgagg tgacgccgct gcgcgcgcat ctgcacggcc acaccagcgt
     4021 gctggtcggg caatccggga tgggcaaatc caccatcgtc aacgcgctcg tgccgggggc
     4081 gcaggcgccc gtgagcgaga tctcggcggc gctcgactcc ggccgccaca ccaccacgca
     4141 tgcgcgcttg taccggctgg atgcgcacag cgcgttgatc gattcgccgg gcctgcagga
     4201 gttcgggctc gcccacgtgc cggtgcgcga gctcgcccgc accttcgtcg aattccggcc
     4261 cctgctcggc cagtgccgct tcggcgactg cctccaccgg ggcgagccgg actgcacttt
     4321 ggccgccgcg gccgaccgcg ggcaagtcag cgagcgacgc ttgcagatct accgccggct
     4381 gctggaggaa gctatgcgcc cgcccagcgc gcgagggtga gcagcaacag cagccccagc
     4441 cacacgatga gcgtgcgcca cagcaggtaa agcgcgctct gcaggtactc ggcatcgggc
     4501 gtcgcgccct cgccgagatc cgggcggaag tcgacgccgg actccagcgg cagcggaccg
     4561 cccaggcgca cgcccagcgc gccggcgccg ctcgccagca gtatgccctg cgccggatcg
     4621 atccacgact gtgcgtagct gcgccaggca gcgatcgcgt cctcgaagtt cccggcgatg
     4681 gcgaagccga ccgccgacaa ccgcaccggc agccagtcga gcacgcgaaa cgcgcgcgcg
     4741 gagaaacgcc cgaaggcgcg gaactcatcg tcccggcgcc gtccccagcg ctgctccatc
     4801 tccgccgcca agcggtagag cagcgctcct gccggcccgg gaagcagtac gaaccagaag
     4861 atcacggcga acacctcgcg gtgcgcgcgc agcagcgcag tctcgatcgc ggccttggcg
     4921 atctcgccct cgccgtaggc gccggccggc tcgccgcgcc actgcagcag caggctgcgc
     4981 gcgcgctcga gatcgccggc gcgcagcgca tcgaagatgc ccttcagggc ctgcaccagc
     5041 tcgcgcaatg cgatgcaggt gtacagcacc gccaccgtcc agacccagcc gagcaccggg
     5101 ttcagccagt gcagcagata gaacaccatc agcgccgcca gcacccacgg gcacaccgcg
     5161 gcgaaccagc ccacggtgcc gtgcgacacc ttgcccgcgt tgaggtcgtg ggccaggcgg
     5221 tcggcgtagc cgcggaaggc ggcgagcagc gcgctgcccc atggcagggg ccgcgtccgt
     5281 tcgatcaaca gcgccgcgat gatggtgaag aaggacacag gatctgggat ctggaagcga
     5341 ggatctgggg tctagcgtct agggggcgtc gagcggcttt gtcttcccca gatcctagct
     5401 cctggctcct ggctcctcaa gtcgtcagac gcaaatacag cgccggcagc gtctcgggca
     5461 ggcgtcggag ctggtccagc accatgtagt tgcgcgcccc gaagatgcgc acgacatatt
     5521 cgtcggcggc cggatccagg ctgatgcaat aggtggcgat gccgtagcgg cgctggtcct
     5581 ccaccgcgcg cttggtgtcg agcaccaggt actgcttgtc cagcacgtcg atgtcgtgcg
     5641 gctcgccgtc tgtaagcaac agcaccaggc gttgcgcgac acggcgcccg cgcaggaagt
     5701 ggccggcgtg ccgcagcgcg catcccatgc gggtggacag ctggccgcgc atgcccgcga
     5761 gccgcgcgcg ggcctgctcg ccgtagggct cgtcgaagtc cttgaagcgg tagtactcga
     5821 cgccgtggcg cccgttcgag tcgaagccgt ggatggcgaa gctgtccccc agatcgtgca
     5881 tggcgtgcgc gaccagcgcg gtggcctcgc gcgccaggtt gagcacggtg ctgcccgccc
     5941 ccggcaccca gtcgttggtg gattgcgaca gatcgagcag cagcagcacg gccaggtcgc
     6001 ggttgttgcg gcccagccgc tgatgcacgc gcgggtccgg cgtgcggccg ctgcgcagat
     6061 cgagcgtcgc ggcgatgcag gcgtcgaggt ccaggcggtc gccctcgagc tgcttgcgca
     6121 ggcgcctggg gcgctggatc tgcaccgcct tgatcagcgt ggtcaggcgc tgcacggtct
     6181 cttcgttccg gcgcagcacc tcgtcgatcg cgcgcgggtc gcccgccggt ggcagcttct
     6241 cgagcagcgt gcaccagcgc cgccgctccg caccgatcag gtaatcccat tcgtcgtaca
     6301 ggtactgctg cgcgggcgcc tcctcgacgc tggccgcgcc ctcggccgct gctgcgtcgc
     6361 tcggcacggc atccggctgc acctgttcgc cccccgcgtg ctccagctcg gtctgctcgc
     6421 cggcatcttc caggctcatg gcgaccgctt cgtaggtcac cgcgcgatct tcgcggtgct
     6481 ctcccgcgtc gcccaggtcc cacaggaaca gattgtcgtc g
//
LOCUS       DASPGE010000285         4890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_192123, whole genome shotgun sequence.
ACCESSION   DASPGE010000285 DASPGE010000000
VERSION     DASPGE010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192123"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VD791_09170"
     CDS             complement(<1..289)
                     /locus_tag="VD791_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXV08188.1"
                     /translation="MRSRTDFETWTQARQARVEQVLDGLLPPAGLSPARLHAAMRYAA
                     LSGGKRVRPLLAYAAGELTAAPEARVDRAAAAVELIHAYSLVHDDLPCMDDD"
     gene            complement(273..572)
                     /locus_tag="VD791_09175"
     CDS             complement(273..572)
                     /locus_tag="VD791_09175"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HXV08189.1"
                     /translation="MPKPSSAPPHAPEDGAAPAAASPSPESTPLSFESALAELEAIVA
                     RMESGELALEQALGAYKRGAQLLQFCQAALKDAQQQVKILEAGLLQDFTGGAEQD"
     gene            750..1103
                     /locus_tag="VD791_09180"
     CDS             750..1103
                     /locus_tag="VD791_09180"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HXV08190.1"
                     /translation="MISVQHEGRLTVAAVFGKFELADYRRFEEEVSRQIAHQGQLNLL
                     IDLRDMLGFTVDVALEDIRFAREHARDVGRIAILSERDAVAWSTLLAQLFVDTEMRVF
                     DDEALARTWLAGEPS"
     gene            1120..1965
                     /locus_tag="VD791_09185"
     CDS             1120..1965
                     /locus_tag="VD791_09185"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HXV08191.1"
                     /translation="MDYRTLIPADELRAQLAAPDLVIFDCRHDLMNPDAGAHAYAHGH
                     IPGARFARSDGDLAGPKTGRTGRHPLPDPAVFMIWLGRNGVDAGKQVVAYDHAGGASA
                     TRLWWMLRHWLGHERAALLDGGWEAWVRAGGPVTAELPRIAAARFGGTPRPAWVDVDY
                     VRAHLNDPDVTLVDARSPERYRGETEPIDPVAGHIPGARNRLYKENLDASGRFKPAAA
                     LRAEFSALLDGRRPDQIVHQCGSGVSACHNILAMEIAGLTGSRLYPGSWSEWCADPSR
                     PVARG"
     gene            complement(1987..2937)
                     /locus_tag="VD791_09190"
     CDS             complement(1987..2937)
                     /locus_tag="VD791_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HXV08192.1"
                     /translation="MRKTIACAASAAALFACPGAHAACGVSFCAVNTDWAVQGEWTKR
                     GGRVDLRFEYIDQDQPRAGTDEVAVGEVPAHHDEVRTINRNWIATFDYNFSAKWGVSA
                     VVPLVDRSHEHIHNHNGAQLPESWNFTEIGDARVTGRYRFASWAGGLGAAGVNFGVKL
                     PTGDYEVTNDDGDAAERSLQPGTGTTDALLGVFYSRARPTGSSWFAQLRVEAALDERD
                     DYRPGSRLFADVGWRARLWGPLAWEIQLNGVVRDRDDGDEAEPEDSGGEFVYVSPGLS
                     VALGPSAQVYLFLQLPLYQHVNGVQLTADWALASGVSLRF"
     gene            complement(3099..3637)
                     /locus_tag="VD791_09195"
                     /pseudo
     CDS             complement(3099..3637)
                     /locus_tag="VD791_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829335.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(3634..>4890)
                     /locus_tag="VD791_09200"
     CDS             complement(3634..>4890)
                     /locus_tag="VD791_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV08193.1"
                     /translation="GPAPLSEHLQRLLWLRGVLLAVLAVALPLAALGHGIALPWGWML
                     AVAAITAALTLHAALRLRRQRPVTERGLFLQLLVDVGALSALLYLSGGPSNPFVSLYL
                     LPLVIAATTLPQRYAWALTGLTAGCYAALFVLPASVPHAEHQFGLHLTGMWVNFVLSA
                     VIICVFVVRMAEAIRRRDALLAAHKEEALRNERIVALGTLAAGAAHELGTPLSTMAVL
                     LREMADELRDDARYVGDIETLRRQVGACKDTITRLSAAAGQPRAEGGAAQPLDRFLLD
                     TVDKWRMIRPAASLTARLDGARPAPAIVGEQTLQHAIVNLLNNAADASPDRVELECDW
                     DAQRLHLEIRDRGQGLTPEARECAGRTLFTTKPSGAGSGIGLLLARATLERLGGRLSL
                     RDRAGGGVITSLELPLHALAPTEPGR"
BASE COUNT          709 a         1783 c         1725 g          673 t
ORIGIN      
        1 cgtcgtcgtc catgcagggc agatcgtcgt gcaccagcga gtacgcatgg atcagctcca
       61 cggcggctgc ggcgcggtcc acgcgcgctt ccggcgccgc ggtcagctcc cccgcggcgt
      121 aggccagcag cggccgcacg cgcttgcctc cgctcagggc ggcgtagcgc atggccgcgt
      181 gcaggcgcgc gggcgacaga ccggcaggtg gcagcagccc gtcgaggacc tgctcgacgc
      241 gggcctggcg ggcctgcgtc caggtctcga agtcagtcct gctccgcacc gcccgtgaag
      301 tcctgaagca gcccggcctc gagaatcttg acctgctgtt gcgcatcctt gagggccgcc
      361 tggcagaact gcagcagctg cgccccgcgc ttgtaggcgc cgagcgcctg ctcgagcgcg
      421 agctcgcccg attccatgcg cgcgacgatg gcttccagct cggccaatgc actctcgaac
      481 gacagcggcg tgctttccgg cgacggcgac gcggcagcgg gggcggcccc gtcttcgggc
      541 gcgtgcgggg gagcggagga tggcttgggc atgggtgcgc ggcccgcata gcgggacgcg
      601 gcgggcaaaa aggggttaaa aatagccgaa catggaagcg ccggtcaaat ttcggcgcgg
      661 ccgcgccggc gcgcggcctg ctaagatacc ctcgtcattc cagttccggc gcgatccgtt
      721 cggcgcctgc gcctgcagcg aagcctcgca tgatctcggt tcagcacgaa ggacgtctca
      781 ccgtggccgc cgtcttcggc aagttcgagc tggccgatta ccggcgcttc gaggaggaag
      841 tgagccgcca gatcgcgcac cagggacagc tcaacctgct gatcgacctg cgcgacatgc
      901 tcggcttcac cgtggacgtc gcgctggaag acatccgctt cgcgcgcgag cacgcgcgcg
      961 acgtgggccg catcgccatc ctgagcgagc gcgatgcggt ggcctggagc acgctgctcg
     1021 cgcagctgtt cgtcgacacc gagatgcgcg tgttcgacga cgaggccctg gcgcgcacct
     1081 ggctcgccgg cgagccatcc tgagcgcgaa gggaggacca tggattaccg caccctgatc
     1141 cccgcagacg agctgcgagc gcagcttgcc gcacccgacc tggtgatctt cgactgccgg
     1201 cacgatctga tgaatcccga cgccggcgcg cacgcctacg cgcacggcca catcccggga
     1261 gcgcgcttcg cccgcagcga cggcgacctg gcgggaccga agaccggccg gaccgggcgc
     1321 catcccctgc ccgacccggc cgtgttcatg atctggctgg gccgcaatgg cgtggacgcc
     1381 ggcaagcagg tcgtggccta cgaccacgcc ggcggcgctt cggccacgcg cctgtggtgg
     1441 atgctgcgcc actggctcgg ccacgagcgt gccgcgctgc tcgacggcgg ctgggaagcg
     1501 tgggtcagag ccggtggacc ggtgaccgcc gagctgcccc gcatcgcggc cgctcgtttc
     1561 ggcggcacgc cgcgccccgc gtgggtggat gtggactacg tgcgcgcgca cctcaacgac
     1621 cccgacgtca cgctggtcga cgcgcgctcg ccggaacgct accgcggcga gaccgagccg
     1681 atcgatccgg tggccggcca tatccccggc gcgcgcaacc gcctgtacaa ggaaaacctg
     1741 gatgccagcg ggcgcttcaa gccggccgcc gccctgcgcg cggagttcag cgccctgctc
     1801 gacggcaggc gccccgatca gatcgtgcac caatgcggct cgggcgtgag cgcctgccac
     1861 aacatcctgg cgatggagat cgccgggctc accggctcca ggctgtatcc cggctcctgg
     1921 agcgagtggt gcgcggatcc gagccggccc gtggcacgcg ggtgatcgcg cacgccttgc
     1981 cgccggtcag aacctcaggc tcacgccgct ggcgagcgcc cagtccgcgg tgagctgcac
     2041 gccgttcacg tgctgatata gcggcagctg caggaacagg tacacctgcg ccgaggggcc
     2101 cagcgcgacg ctcagtcccg ggctgacgta cacgaactcg ccgccgctgt cttccggctc
     2161 ggcctcgtcg ccgtcgtccc ggtcgcgcac cacgccgttc aactggatct cccaggcgag
     2221 cggaccccac aggcgggcgc gccagcccac atcggcgaac aggcggctgc cggggcgata
     2281 gtcgtcgcgc tcgtcgagcg ccgcctcgac gcgcaattgc gcgaaccacg acgagccggt
     2341 gggacgtgca cggctgtaga acacgccgag cagggcatcg gtggtcccgg tgccgggctg
     2401 cagggagcgc tcggcggcat cgccgtcgtc gttggtgacc tcgtagtcgc cggtcggcag
     2461 tttcaccccg aagttcacgc cggccgcgcc caggccgccg gcccacgaag cgaagcggta
     2521 gcgcccggtc acgcgcgcat cgccgatctc ggtgaaattc cagctctccg gcaattgcgc
     2581 gccgttgtgg ttgtggatgt gctcgtggct gcggtccacc agcggcacga ccgccgacac
     2641 gccccacttg gcgctgaagt tgtagtcgaa ggtcgcgatc cagttgcggt tgatggtgcg
     2701 cacctcgtcg tggtgcgccg gcacctcgcc gaccgcgacc tcgtcggtgc ccgcgcgcgg
     2761 ctggtcctgg tcgatgtact cgaagcgcag atcgacgcgc ccgccgcgtt tggtccactc
     2821 tccctgcacc gcccagtcgg tgttcaccgc gcagaaggaa acgccacacg cggcgtgggc
     2881 gcccgggcag gcgaacagcg ccgccgccga cgcggcgcag gcgattgttt tcctcatcgc
     2941 ttcttcccct cgtgagcgcg agtctaggcg ggggagccgg cgggcgacag cgaccgattg
     3001 ccgcatgcgg caaactgtcg cacctgcgtt gcgcgcatcg gccatcgcgc tacgaccgcg
     3061 ctttccggcc gcgcagcggg cggtctgtcg cgaaccgctc agtcccgaac cgggcgcttg
     3121 gcgagcttgc gctgcagcgt gcgccggtgc atgcccaggc tgcgggccgt ggccgacacg
     3181 ttaccgccgt tgtcgcgcag gacgcgctgg atgtgctccc actccaggcg gttcaccgac
     3241 agcgggcgct ccgccaccgg cacgccggcg tcgccgttgg tgcgttggtg gatgcggcga
     3301 gcagctcttc cgcgtccacc ggcttggcga ggtagtgcgt ggcccccagc ttgatcgcct
     3361 ccaccgcggt ggcgacgctg gcatagccgg tcagcacgac gatgcacatc ccgggcgcca
     3421 gggcccgcag cccggcgatc agcgccagac cggactcgcc gggcatcttc agatcgacca
     3481 ccgcccactc cggaggatcg gtggcggcct gcgccatcgc ggtctcgacg ctgtgcgcgg
     3541 tgcgcaccag gtggccgcgg cgcgcgaggg cacgctgcag cacggcgcag aacgtggtgt
     3601 cgtcgtccac cagcagcacc tggcggcccg cgctcatcgg cccggctccg ttggcgccag
     3661 cgcgtgcagg ggcagctcca gcgaggtgat cacgccgccg ccggcccggt cgcgcaggct
     3721 gagccggccg ccgaggcgct ccagcgtggc gcgcgcgagc agcagcccga tgccgctgcc
     3781 ggcgccgctg ggcttggtgg tgaacagcgt gcgaccggcg cactcgcgcg cctcgggcgt
     3841 gaggccctgg ccgcggtcgc ggatctccag atgcaggcgc tgcgcgtccc agtcgcactc
     3901 cagctccacg cggtccggtg aagcgtccgc ggcgttgttc agcagattga cgatggcatg
     3961 ctgcagcgtc tgctcgccga cgatcgccgg cgccgggcgc gccccgtcca gccgcgcggt
     4021 gaggctcgcc gccggccgga tcatgcgcca cttgtccacc gtgtccagca ggaaccgatc
     4081 caggggctgg gcggcgccgc cctccgcccg cggctgtccg gcggccgccg acaggcgcgt
     4141 gatcgtgtcc ttgcaggcgc ccacctggcg gcgcagcgtc tcgatgtcac cgacatagcg
     4201 cgcgtcgtcg cgtaactcgt cggccatctc gcgcagcagc accgccatcg tcgacagcgg
     4261 ggtgcccagc tcatgggccg caccggcggc gagcgtgccg agcgcgacga tgcgctcgtt
     4321 gcgcagcgcc tcctccttgt gcgcggcgag cagcgcgtcg cgccggcgga tcgcctccgc
     4381 catgcgcacg acgaacacgc agatgatcac cgccgagagc acgaagttca cccacatccc
     4441 ggtcaggtgc aggccgaact gatgctcggc gtggggcacg gaagcgggca gcacgaacag
     4501 cgcggcgtag cagcccgccg tcaggccggt cagcgcccag gcgtagcgtt gtggcagcgt
     4561 ggtcgccgcg atcaccagcg gcaacagata cagggatacg aacgggttgc tcggaccgcc
     4621 gctcaagtag agcagcgcgg acagcgcgcc cacgtccacc agcaattgca ggaacagccc
     4681 gcgctcggtg accggacgct gccgccgcag ccgcaaagcc gcgtgcaagg tcagcgcagc
     4741 cgtgatcgcc gccaccgcca acatccagcc ccaaggcagc gcgatgccat ggcccagggc
     4801 cgcgagcggc agcgccacgg ccagcacggc gagcagcacc ccgcgcagcc acagcaggcg
     4861 ctgcaggtgc tcggacaagg gagcggggcc
//
LOCUS       DASPGE010000286         7545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1129288, whole genome shotgun sequence.
ACCESSION   DASPGE010000286 DASPGE010000000
VERSION     DASPGE010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7545
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129288"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VD791_09205"
     CDS             <1..991
                     /locus_tag="VD791_09205"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV08194.1"
                     /translation="RPEVAWTIPLSLGDSHRGFRVLGTTSDYFEHLRFARERRLVLAQ
                     GVPLADLHDAVLGADVAQALGYGLGRSIVIAHGAGEISFALHEELPFRVVGILARTGT
                     PVDRTVHISLEGLEAVHAEALAGAEDPLAAALHAQDDHSEHHAPSAITAFLVGLKSRP
                     AALGLQRIVNQYPGEPLTAILPAATLQEVWEITGAAERALFAVSALVVVVGLAGMLVA
                     LLTGLSERRREMAVLRAVGARPAHVFGLLLGEAAFLTALGIALGFAALYLGLTLGQPW
                     LESRLGLFIAVGWPSRHELSLAALVAGAGVLIGLIPAWRIYRYSLADGMTVHV"
     gene            1004..1663
                     /locus_tag="VD791_09210"
     CDS             1004..1663
                     /locus_tag="VD791_09210"
                     /inference="COORDINATES: protein motif:HMM:NF023164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HXV08195.1"
                     /translation="MEFRNLLPAVLMAALLAPVALADTPRQLTWADLVPKAGTLDEAL
                     AKLTRDQLAALAELASVRDRKARGEKLSAADVADEQALSRKLDQAGVNVEDLLARREE
                     LAAKRQAQKNAVDPALNGTLVRIPGYLLPLEFRGKAVSEFLLVPWVGACIHTPPPPPN
                     QIVHVKTEKAFEVSSIFMAVWVTGQMSTGATKKSLFLIDGTSDIDIGYSMRATEVEPY
                     K"
     gene            complement(1716..2279)
                     /locus_tag="VD791_09215"
     CDS             complement(1716..2279)
                     /locus_tag="VD791_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08196.1"
                     /translation="MSAHPKTSRRRPSLRALVLGAWIGVAAAVATPSIATACVDGAPK
                     GEAVMVQRDVGTTAAGEHAEFHVLGAGWFGAAEVVKQGGTSDDTVVTVELDGAELITG
                     SFASLKNPWMQINTPFLVVNVRSEGDARTMTIWFSPELKFRAILAVRVEVREEGVAGL
                     RLRAVMNKPGPHEDPVGQTALVLPAFK"
     gene            2399..4957
                     /gene="glgP"
                     /locus_tag="VD791_09220"
     CDS             2399..4957
                     /gene="glgP"
                     /locus_tag="VD791_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HXV08197.1"
                     /translation="MQPRTVFQLEVNPKLPQRLARLEELASNLWYSWDRPTRTLFSRL
                     HTGLWDAVGHNPKAFLRRVDEQVLVDAADDPVFLANYNRVLSAYDTYHSEPLRRNGSE
                     WLRAADVVAYFCAEFGFHESFPIYSGGLGILAGDHCKAASDMRLPFVGVGLLYRQGYF
                     SQSIDTEGNQHATYTDSEFESLPVSPVLRADGSELKVHVDLPERQVACKVWHTRCGHV
                     RLYLLDTDLEENDQLGRDITHQLYGGDRHMRIEQEIVLGVGGVRALEQLQIKPTVWHI
                     NEGHAAFLVLERMRALAGQGLDCAAALESVASNTVFTTHTPVPAGHDHFAEEMIWGYF
                     QRFCTELGMSREAFLELGRVDGRREFNMTALAIRTSRYHNGVSRIHGAVSSRICSDLW
                     PQVDHQENPIGYITNGVHVPTFLAQEWGEVFEKHLGFDWSQRLTDAAFWQRVDEIPDH
                     LFWSVRQSLKAQMLHLVRFRVARQHFRNRGSEAHLDRLLKFADPVNPNVLTIGFARRF
                     ATYKRAALLFEDLEWLRQMMCNNERPVLLIFAGKAHPADQPGQELIRRITHIAAMPQF
                     EGRILLVEDYDLRLSRRLVSGVDVWLNNPVYPLEASGTSGMKAGINGVLNLSVLDGWW
                     DEGYDGKNGWAIKPVSESFEEARRNREEAKALYELLQDHVIPMYYRRSDMGFSQDWIR
                     MAKRSIASLLPRFSATRMVGEYLAKFYLPATRQGRRYSDAGFAAARKVAAWKAHVRKS
                     WHDVRLRRLDSGPRNIVYGDSLRIEVAVNLNGLKPEDVVVEMLLGRQTESEKLRESRR
                     YRFEWEGVMTDQGEHRFVLQLTPEMCGKLDYRVRAYAWHEMLTHPFEMGMMRWL"
     gene            5128..6546
                     /locus_tag="VD791_09225"
     CDS             5128..6546
                     /locus_tag="VD791_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HXV08198.1"
                     /translation="MAEHRKYVAFKGKLLMIGFGAVGQGTLPLILRHIDMPKERIKII
                     TADDWGRDIAEGYGVAFAVEPLVRENYLAILDRELRRGDFLFNASFDVSSLALIEYCS
                     ERGILYLDACIEPWAGGHRDTSVPAARRTNYAFREAALEIGQRHPKGATCVVMHGANP
                     GLVSHFVKQALLNIARDTRAGLPEPATQEQWGRFAMQLGVKTIQIAERDRQVGSRRKE
                     WGEFVNTWSSGAFVGESLQPAELGWGTHEKHWPREGMEFGFGCGSSIYLNRPGCTTRV
                     RSWVPTEGPFHGWLISHGETHSIPDYFTVRENGRAVYRPTCYYAYHPCDDAVLSLHEM
                     VGHNFSMQERTRLLREEITDGIDELGVLLMGHARGAYWYGSQLSIHEARQLCPHNNAT
                     SLQVNAPALGAVVWAMRNPAASVVEPDDLDFRTVLEVAMPYLGPVVGVYSDWTPLEGR
                     AKLYPEDVDRDDPWQFKNFLVT"
     gene            6584..>7545
                     /locus_tag="VD791_09230"
     CDS             6584..>7545
                     /locus_tag="VD791_09230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HXV08199.1"
                     /translation="MTGLLTRILEALGGATRLAVRITFADGSSWENQPGEPVVHLRFK
                     TRSAQWRTLALGHIGLLESYFDQSLDVEGDFARALRIGFDSRLAESPRWPVRVRNQWH
                     ELTRSNATLAQAKANARFHYGLGAEFYSQWLDRELMMYTCAYWKDGTRTVEQAQRNKV
                     EHVCRKVRLQPGESVVDIGCGFGGFMFHAWEHHGARVCGVNTTTEQVDWLREEIGRRG
                     LDDKLEVREADFREVHAQFDKVISIGVLEHAGRDQLREVVKAHADFLKPGGLGMLHFI
                     GHVGVRHTEFYIREHVFPGGWIPSLAETICAMEECGLEIVDIENL"
BASE COUNT         1227 a         2559 c         2561 g         1198 t
ORIGIN      
        1 gcgcccggaa gtcgcctgga ccattccgct gtcgctcggc gattcccacc gcgggttccg
       61 cgtgctcggc accacgtccg actatttcga gcacttgcgc ttcgcgcgcg agcgccggtt
      121 ggtgctcgcc cagggcgtgc cgttagccga cctgcacgac gcggtgctgg gtgcggatgt
      181 ggcgcaagcg ctgggttacg ggctgggccg ctccatcgtc atcgctcacg gtgcgggcga
      241 gatcagcttc gcgctgcacg aggagctgcc gttccgggtg gtggggattc tcgcgcgcac
      301 cggtactccg gttgaccgca cggtgcacat cagcctggaa ggcctcgagg ccgtgcacgc
      361 cgaagcgctc gccggcgccg aagatccgct cgcggcggcg ctgcacgcac aggacgacca
      421 ctcggagcac cacgccccga gcgcgatcac cgctttcctg gtcgggctga aatcgcgtcc
      481 cgcggcgctc ggcctgcagc gcatcgtcaa ccagtatccc ggcgagccgc tcaccgccat
      541 cctgccggcc gccacgctgc aggaggtgtg ggagatcacc ggcgcggcgg agcgcgcgtt
      601 gttcgcggtg tccgcgctgg tggtggtcgt ggggctggcc ggcatgctgg tagcgctgct
      661 caccggcttg agcgagcgcc gccgggagat ggcggtcttg cgcgccgtcg gggcgcggcc
      721 tgcgcacgta ttcgggctgc tgctgggcga ggccgctttc ctcaccgcac tcggcatcgc
      781 gctcgggttt gccgccctct atctcggcct gacgctcggc cagccctggc tggagtcgcg
      841 cctggggctg ttcatcgccg taggctggcc atcacgccac gagctgtcgc tcgcggcgct
      901 ggtagcggga gcgggcgtgc tgatcggcct cattcccgcc tggcgcatct accgctactc
      961 gctggccgac ggcatgaccg tgcacgtctg atagagtgat gccatggagt tcaggaacct
     1021 gttgcctgcc gtcctgatgg cggcgctgtt agcaccggtc gccctggcgg acacgccgcg
     1081 gcaactgacg tgggcggacc tggtacccaa ggccggcacc ctcgacgagg ccctggccaa
     1141 actcacgcgc gatcagctcg ctgcgctggc ggagctcgcc tcggtgcgcg accgcaaggc
     1201 gcgaggagag aagttgagcg cggccgacgt cgccgacgag caggcactgt cgcgcaagct
     1261 cgaccaggca ggcgtgaacg tcgaggatct gctcgcccga cgcgaggagc tggcggcgaa
     1321 acgccaggcg cagaagaatg cggtcgaccc ggcactgaac ggcacgctgg tgcgcattcc
     1381 cggttacctg ctgcctctcg agttcagggg caaggcggtg tcggagttcc tgctggtgcc
     1441 ctgggtcggc gcctgcatcc acactccgcc gcctccgccc aaccagatcg tccatgtgaa
     1501 gacggaaaag gccttcgagg tctccagcat cttcatggca gtctgggtca cggggcagat
     1561 gtccaccggc gccaccaaga agtccctgtt cctgatcgac gggacctccg acatcgacat
     1621 cggttattcg atgcgggcga ccgaggtgga gccctacaag tagttggtcg cgaagcgacc
     1681 tgccggggcc gcaacgcgcg cgcaggcgag aagcgctact tgaatgcggg cagaacgagc
     1741 gccgtctggc cgacggggtc ctcgtgcggg ccgggcttgt tcatgacggc gcgcagccgc
     1801 aaacccgcca caccctcctc ccggacctcc acgcgcaccg ccaggatggc cctgaacttc
     1861 agctccggcg aaaaccagat ggtcatggtg cgggcgtcgc cttcgctgcg cacgttgacc
     1921 accaggaatg gcgtgttgat ctgcatccac gggttcttca gggaggcaaa cgagccggtg
     1981 atcagctccg cgccgtcgag ctcgacggtg acgacggtgt cgtcgctggt cccgccctgc
     2041 ttgacgacct cggcggcgcc gaaccacccg gcccccagca cgtggaactc ggcgtgctcg
     2101 cccgcggccg tggtgcccac gtcgcgctgg accatcaccg cttcgccttt gggcgcgccg
     2161 tccacgcatg ccgtcgcgat cgacggggtc gccacagcag cggcgacgcc gatccaggcg
     2221 cccaggacca gcgcccgcag cgacggtcgc cgccttgaag tcttagggtg tgcgctcact
     2281 gaagtgtgac gaaccgccct tcggggcgcc cgtctattgc cgatatgatg cagcgtagca
     2341 taattcgccc cgaatcggcg cctcgccccg ataagaatcc atcgccaccc gctcaccgat
     2401 gcagcctcga accgtcttcc agctagaagt caatcccaag ctcccgcagc gcctggcacg
     2461 gctcgaggag ctggcctcca acctctggta cagctgggac cgccccaccc gcaccctgtt
     2521 ctcgcgcctg cacaccgggc tgtgggatgc ggtcggtcac aatcccaagg cgttcctgcg
     2581 ccgggtggac gagcaagtcc tggtcgatgc cgcggacgac ccggtattcc tggcgaacta
     2641 caaccgggtg ctgtcggcct acgacacgta tcacagcgag ccgctgcggc gcaacggctc
     2701 ggaatggctg cgcgccgcgg acgtggtggc gtatttctgc gccgagttcg gcttccacga
     2761 gagcttcccg atctactccg gaggcctggg catcctggcg ggcgaccatt gcaaggcggc
     2821 gagcgacatg cgcctgcctt tcgtcggggt cgggctgctc taccgccagg ggtacttctc
     2881 ccagagcatc gacaccgagg gcaaccagca cgcgacgtac accgactccg agttcgagag
     2941 cctgccggtg tcgccggtgc tgcgcgccga cggctccgag ctcaaagtgc acgtcgacct
     3001 gcccgagcga caggtcgcgt gcaaggtctg gcacacgcgc tgcggccatg tgcggctgta
     3061 cctgctggat accgatctgg aggagaacga ccagctcggc cgcgacatca cccatcagtt
     3121 gtacggcggc gaccgccaca tgcgcatcga gcaggagatc gtgctcggcg tgggcggggt
     3181 gcgcgctttg gagcagctgc agatcaagcc gaccgtgtgg cacatcaacg aaggccatgc
     3241 cgccttcctg gtgctcgagc gcatgcgcgc gctggccggc caagggctgg attgcgccgc
     3301 cgcgttggag tcagtggcgt ccaataccgt gttcaccacc cacaccccgg tccccgccgg
     3361 gcacgaccac ttcgccgagg agatgatctg gggctacttc cagcgtttct gcacggagct
     3421 gggcatgagc cgggaggcgt tcctcgagct gggccgggtc gacggccgcc gggaattcaa
     3481 catgaccgcg ctggcgattc gcacgtcgcg ctaccacaac ggcgtgagcc gcatccacgg
     3541 cgcggtctct tcgcgcatct gcagcgacct gtggccgcag gtcgaccacc aggagaaccc
     3601 gatcggctac atcaccaacg gcgtgcatgt accgaccttc ctcgcccagg aatggggtga
     3661 ggtgttcgag aagcacctcg gcttcgactg gagccagcgc ctcaccgatg ccgctttctg
     3721 gcagcgggtg gacgagatcc cggaccatct gttctggagc gtgcgccagt cgctcaaggc
     3781 gcagatgctg cacctggtgc gcttccgcgt ggcacgccag catttccgca accgcggctc
     3841 ggaggcccac ctcgaccggc tgctgaagtt cgccgacccg gtgaacccga acgtgctcac
     3901 catcggcttc gcccggcgct tcgccaccta caagcgcgcc gccctgctgt tcgaggacct
     3961 ggaatggctg cggcagatga tgtgcaacaa cgagcgcccg gtgctgctca tcttcgccgg
     4021 caaggcgcac ccggccgatc agccgggcca ggagctgatc cgccgcatca cgcacatcgc
     4081 ggcgatgccg cagttcgagg gccgcattct gctggtcgag gactacgatc tgcgcctgtc
     4141 gcggcggctg gtgtcgggcg tggacgtatg gctcaacaat ccggtctacc cgctggaggc
     4201 gagcggcacc tcgggcatga aggccggcat caacggcgtg ctcaacctgt ccgtgctcga
     4261 cggctggtgg gacgagggct acgacggcaa gaacggctgg gcgatcaaac cggtatcgga
     4321 gagcttcgag gaggcacgcc gcaaccgcga ggaagccaaa gcgctgtacg agctgctgca
     4381 ggaccacgtc atcccgatgt actaccggcg cagcgacatg ggcttctcgc aggactggat
     4441 ccgcatggcg aagcgctcca tcgccagcct gctgccacgc ttctcggcca cgcgcatggt
     4501 gggcgagtat ctcgccaagt tctacctgcc ggccacccgc cagggtcggc gctacagcga
     4561 cgccggcttc gcggcggcgc gcaaagtcgc cgcctggaag gcgcacgtgc gcaagtcctg
     4621 gcacgacgtg cgcctgcgca ggctggacag cgggccgcgc aacatcgtct acggcgacag
     4681 cctgcgcatc gaggtggcgg tgaacctgaa cggcctcaag ccggaagacg tggtggtcga
     4741 gatgctgctc ggcaggcaga ccgaatcgga gaagctgcgc gagagccgcc gctatcgatt
     4801 cgagtgggaa ggggtgatga ccgatcaggg cgagcaccgt ttcgtgctgc agctcacccc
     4861 ggagatgtgc ggcaagctgg actacagggt gcgcgcttac gcctggcacg aaatgctcac
     4921 gcatccgttc gagatgggga tgatgcggtg gctctaggca cgaggcctga ggcgcgagca
     4981 gactcccttc tccccttgcg ggagagggtc gcggtcgccg accgctagac acgccgcgaa
     5041 aaatccttac aattgcgccc tcgcgccggc acccccttcc cgggaggccg gctttttcgc
     5101 gcctggacgc gcaggcgggg gaagcacatg gctgagcaca ggaaatacgt cgccttcaag
     5161 ggcaagctgc tgatgatcgg ctttggggcg gtcgggcagg gcacgctgcc gctgatccta
     5221 cggcacatcg acatgccaaa ggagaggatc aagatcatca ccgccgacga ctggggccgc
     5281 gacatcgccg aaggctacgg cgtcgccttc gcggtcgagc cgctggtgcg ggagaactac
     5341 ctggcgatcc tggaccgcga gctgcggcgc ggcgatttcc tgttcaacgc ttcgttcgac
     5401 gtctccagcc tggcgctcat cgagtactgc agcgagcgcg gcatcctgta cctggacgcc
     5461 tgcatcgagc cctgggcggg cggccaccgc gacacctcgg tgccggccgc gcgccgcacc
     5521 aattacgcct tccgcgaggc cgcgctcgag atcggccagc gccacccgaa gggggccacc
     5581 tgcgtggtga tgcacggcgc caatcccggg ctggtgtcgc acttcgtcaa gcaggcgctg
     5641 ctcaacatcg cacgcgacac ccgcgccggg ctgcccgaac ccgccacgca ggagcagtgg
     5701 ggccgcttcg ccatgcagct cggggtcaag acgatccaga tcgccgagcg cgaccgccag
     5761 gtgggcagcc ggcgcaagga gtggggcgag ttcgtcaaca cctggtcgag cggcgccttc
     5821 gtgggcgagt cgctgcaacc cgccgagctg ggctggggta cgcatgagaa gcactggccg
     5881 cgcgagggca tggagttcgg cttcggctgc ggctccagca tctatctgaa ccgcccgggc
     5941 tgcaccacgc gcgtgcgctc ctgggttccg accgagggcc cgttccacgg ctggctgatc
     6001 tcgcacggcg agacgcactc gatccccgac tacttcacgg tgcgcgagaa cgggcgcgcg
     6061 gtctaccggc cgacctgcta ctacgcctac cacccctgcg acgacgcggt tctgagcctg
     6121 catgagatgg tgggccacaa cttcagcatg caggagcgca cgcgcctgct gcgcgaggag
     6181 atcaccgacg gcatcgacga gctgggggtg ctgctcatgg gtcatgcgcg cggtgcctat
     6241 tggtacggct cgcagctctc gatccacgaa gcgcgccagc tgtgcccgca caacaacgcc
     6301 accagcctgc aggtgaacgc gccggcgctg ggcgcggtgg tgtgggcaat gcgcaacccg
     6361 gccgcgagcg tggtggagcc cgacgatctg gacttccgca ccgtgctcga agtggccatg
     6421 ccctacctgg gcccggtggt cggggtgtac agcgactgga cgccgctcga gggccgcgcc
     6481 aagctctacc ccgaggacgt ggaccgggac gacccctggc agttcaagaa cttcctcgtg
     6541 acctgacgta agatacggcg cggtctccga agacgggggg acgatgaccg gcctgctcac
     6601 acgcatcctc gaagcacttg gcggggccac gcggctcgcc gtgcggatca ccttcgccga
     6661 cggctcctcc tgggaaaacc agccgggaga gccggtggtg catcttcgct tcaagacccg
     6721 gagcgcgcag tggcgcaccc tggcgcttgg ccacatcggg ctgctcgagt cctacttcga
     6781 ccagtcgctc gatgtcgagg gcgacttcgc gcgcgccctg cgcatcggct tcgacagccg
     6841 gctcgccgag tcgcccagat ggccggttcg cgtgcgcaac caatggcacg agctgacgcg
     6901 ctccaacgcc acgctggccc aggccaaggc caacgcgcgc ttccactacg ggctgggcgc
     6961 ggagttctat tctcagtggc tggaccgcga gctgatgatg tacacctgcg cctactggaa
     7021 agacggcacg cgcacggtgg agcaggcgca gcgcaacaag gtcgagcacg tgtgccgcaa
     7081 ggtgcgcctg cagcccggcg agagcgtggt ggacatcggc tgcggcttcg gcggtttcat
     7141 gttccacgcc tgggagcatc acggcgcccg ggtgtgcggc gtcaacacca ccaccgagca
     7201 ggtggactgg ctgcgcgagg agatcggccg gcgcggcctg gacgacaagc tcgaggtgcg
     7261 cgaggccgac ttccgtgaag tgcacgcaca gttcgacaag gtgatctcga tcggggtgct
     7321 ggagcacgcc gggcgcgatc agctgcgcga ggtggtgaag gcccacgccg atttcctcaa
     7381 acccggcgga ttgggcatgc tgcacttcat cggccacgtc ggcgtgcgcc atactgagtt
     7441 ctatatccgt gagcacgtgt ttcccggggg ctggatcccg agcctggcgg aaaccatctg
     7501 cgcgatggag gagtgcgggc tggagatcgt cgacatcgag aacct
//
LOCUS       DASPGE010000287         3434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1129638, whole genome shotgun sequence.
ACCESSION   DASPGE010000287 DASPGE010000000
VERSION     DASPGE010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129638"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..517
                     /locus_tag="VD791_09235"
     CDS             251..517
                     /locus_tag="VD791_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08200.1"
                     /translation="MTKTAERNRKARKAAAKRVAAAKPRAGKAAPVKNAAGARKTPGV
                     KRAAAKKVAGRQAAGGYVVFESPVEPRHFTIEQIRRAVKEVVNG"
     gene            531..1178
                     /locus_tag="VD791_09240"
     CDS             531..1178
                     /locus_tag="VD791_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08201.1"
                     /translation="MAVSSEARFDRCVFVNCPFDSAYKPLLHAILFAIHDCGFIARSA
                     LEATGSAEPRLDKIVRIIRESRWSVHDISRVEVDPDNPLPRFNMPFECGLALGAQRFG
                     TARDRRRDFLILTGARHQDKLTLSDLAGQDAVHHGNVPERAIDAVRAFLAPKSRSQVR
                     GGAAIKARYTRFRAELPRLAAALEIGDQEILSFEYLPDWLRVMNAWLVQGGAPPG"
     gene            1302..2096
                     /gene="surE"
                     /locus_tag="VD791_09245"
     CDS             1302..2096
                     /gene="surE"
                     /locus_tag="VD791_09245"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HXV08202.1"
                     /translation="MRILLSNDDGYFAPGLAVLAEALSKVADIVVYAPERDRSGASNS
                     LTLDRPLIVRKSHLGFYYINGTPTDCVHLAVTGLLDPLPDMVVSGINHGANMGDDTVY
                     SGTVAAATEGFLLGIPSIAISLAKRNDGHYPSAARVAVELVTRFQREPLRQPVLLNVN
                     VPDVPYDKLRGLKVTRLGRRHKAEPTIKTINPRGETVYWVGAAGDAQDAGEGTDFEAV
                     ASDYISVTPLQIDLTQYGQLERVRDWLALRGQDAQPALADTPDAGR"
     gene            2119..2778
                     /locus_tag="VD791_09250"
     CDS             2119..2778
                     /locus_tag="VD791_09250"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HXV08203.1"
                     /translation="MSTRHLGIGMTSQRTRLRMVERLRDQGIRDETVLAAMAEVPRHI
                     FVDEALAHRAYEDIALPIGFGQTISNPWIVARMLELLRSGGALGKALEIGTGCGYQAA
                     VLSKLAREVYSVERISALLMKARVNLRELRISNVRLKHADGQFGLKDAGAFDAIVMAA
                     ATAQVPEALLDQLAPGGRMVLPVGTRLQHLTLVERTPEGLIRTTLDPVRFVPLLPGAH
                     P"
BASE COUNT          541 a         1230 c         1143 g          520 t
ORIGIN      
        1 gatagcggac agacgcaacg cgcccgactt gcctgcttac tggactctct tgtagcgccc
       61 gattccgtgt ctgcacgggc gcgtggctat cggactcgaa ccgatgcgtg attgctgatc
      121 gtcgcttgtg ctcacgagac ctccagccta tactgcgcgc cagcgtatgt tagattcgtc
      181 atcatcggta ccgtcgctct gacgatcgcc agcctgaggt gcaaagctca aagccgcttc
      241 gcgacacatc atgaccaaga ccgccgaacg caacaggaaa gcccgcaagg cggccgccaa
      301 gcgcgtcgcg gcggccaagc cgcgcgccgg caaggccgcc cccgtcaaga acgccgccgg
      361 cgccaggaaa accccgggcg tcaagagagc agcggccaag aaggtcgccg gcaggcaggc
      421 cgcgggcggc tacgtggtgt tcgaatcgcc ggtcgagcct cggcacttca ccatcgagca
      481 gatccggcgc gcggtcaagg aagtcgtcaa cggctagccc ggatcaccgc gtggcggtct
      541 cgagcgaggc acggttcgac cgctgcgtgt tcgtcaactg ccccttcgat tccgcctaca
      601 agccgctgct gcacgcgatc ctgttcgcga tccacgattg cggcttcatc gcgcgcagcg
      661 cgctggaggc gaccggctcc gccgagccca ggctggacaa gatcgtgcgc atcatccgcg
      721 agtcgcgctg gtcggtgcac gacatcagcc gggtggaggt cgacccggac aacccgctgc
      781 cccgcttcaa catgccgttc gagtgcgggc tggcgctcgg cgcgcagcgc ttcggcacgg
      841 cgcgcgaccg gcggcgcgat ttcctgatcc tgaccggtgc gcggcaccag gacaagctca
      901 ccctcagcga tctcgccggc caggatgcgg tccaccacgg gaatgttccc gagcgcgcga
      961 tcgacgcggt gcgcgccttc ctcgcgccga aatcgcgctc gcaggtgcgc ggcggcgccg
     1021 cgatcaaggc gcgctacacc cggtttcgcg ccgagctgcc caggctcgcg gccgcgctgg
     1081 agatcggcga ccaggagatc ctgtccttcg aatacctgcc cgactggctg agggtgatga
     1141 acgcatggct ggtgcagggc ggtgcgcccc cgggttgata tgatcgggat tgatataatt
     1201 caggccgtgc ttctcgccag cgcccggctg ctcccctgct tcgcgttccc ggcgactccg
     1261 tcgacgacag cgaaacggac cgggataccg gccggattcc gatgcgcatt ttgctgagca
     1321 acgacgacgg ctacttcgcc cccggcctag cggtgctggc cgaggcgctg tccaaagtgg
     1381 ccgacatcgt cgtgtacgcg ccggagcgcg accgcagcgg cgccagcaac tccctgacgc
     1441 tggatcgccc gctgatcgtg cgcaagtcgc acctcggttt ctactacatc aacggcacgc
     1501 cgaccgactg cgtccacctg gcggtgaccg gtctgctcga cccgctgccc gacatggtgg
     1561 tgtccggcat caatcacggc gccaacatgg gcgacgacac ggtctattcg ggcacggtgg
     1621 ccgcggccac ggagggcttt ctgctgggca tcccctcgat cgccatctcg ctggccaagc
     1681 gcaacgacgg gcactacccc agcgcggcgc gggtcgcggt ggagctggtg acgcgctttc
     1741 agcgcgagcc cctgcgccag ccggtcctgc tcaacgtcaa cgttccggac gtgccttacg
     1801 acaagctcag gggactgaaa gtcacccgcc tcgggcggcg gcacaaggcc gagcccacca
     1861 tcaagaccat taatccgcgc ggtgagaccg tgtactgggt gggcgccgcc ggcgacgcgc
     1921 aggacgccgg cgagggcacc gacttcgagg cggtggcgag cgactacatc tcggtcaccc
     1981 cgttgcagat cgacctcacg cagtacggtc agctcgagcg cgtgcgcgac tggctggcgc
     2041 tgcggggaca ggacgcgcag cccgcgctgg cggatacgcc cgatgccggc cgctgatggg
     2101 gcggcagccg ctgcggccat gagcacccgg catctgggca tcggcatgac ctcgcagcgc
     2161 acgcgcctgc gcatggtcga gcggctgcgt gaccagggca ttcgcgacga gaccgtgctt
     2221 gccgcgatgg ccgaggtgcc gcgccacatc ttcgtggacg aggcgctggc gcaccgcgcc
     2281 tatgaggaca tcgcgctgcc catcggcttc ggtcagacca tctccaaccc ctggatcgtg
     2341 gcgcgcatgc tcgagctgct gcgcagcggc ggggcgctcg gcaaggcgct cgagatcggc
     2401 accggttgcg gttaccaggc ggcggtgctg tccaagctcg cgcgcgaggt ctactcggtc
     2461 gagcgcatct cggcattgct catgaaggca cgcgtgaact tgcgcgagct gcgcatctcc
     2521 aacgtacggc tgaagcacgc ggacggccag ttcggcctga aggacgccgg cgccttcgac
     2581 gccatcgtga tggcggctgc cacggcgcaa gtgccggaag cgctgctcga ccagctggcg
     2641 cccggtggcc gcatggtgct gcccgtgggc acgcgcctgc aacacctcac attggtggag
     2701 cgcacacccg aaggactcat ccgcaccact ttggacccgg tgcgcttcgt gccgttgctg
     2761 cccggagcgc atccatgaac cgcgggacgt tgccccggcc gacttcccgc gaagctgtcg
     2821 ctcaggatgg agttgcgaga tccggctaga cgaggacgtg cggcgcgctc cggtgcgggg
     2881 cgcgcgctcg ccctgatcgc ccccatcgtc tgcgcgggat gcgggcagcc cggcgcgccg
     2941 gtggtggata ctccggtatg ggacggcgtt ccccggcgcg cggagacggc accaaaaccc
     3001 ccggcaccgc caacttctcc tcccggggcg acctcgcccc cggcggcgac ttctccaccg
     3061 gcccctgcac ctgcgccgcg cacggccgcg acgcccgccc ggccggcggc cgctgttcct
     3121 ccgccggcga catctcctgc tgcggcccct cccgccacag cgacgccggt tccttctgct
     3181 acggcaacgc cggtcccttc tgctacggca acaccggtac cttctgctac ggcaacaccg
     3241 gtcccttctg ctacggcaac accggctacg acagcagcgg cgccttctgt cgcgacggca
     3301 gcggcatccc gcccggcggc cccggcggtg cgcgcgagca ccgaggccga ggcgctgccg
     3361 gagttctaca cggtgaggaa gggcgacacg ctgtacagca tcgcgctgga caacggccag
     3421 gactacaagg acgt
//
LOCUS       DASPGE010000288         9561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_311589, whole genome shotgun sequence.
ACCESSION   DASPGE010000288 DASPGE010000000
VERSION     DASPGE010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311589"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..930)
                     /locus_tag="VD791_09255"
     CDS             complement(205..930)
                     /locus_tag="VD791_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08204.1"
                     /translation="MPSVHRTVKRRLAVLAVVCTASLGVPASADDAARLRLAALAEEA
                     ELLQEELAGLQPTIDRLRTEGERLADSEAALRSESLALEREIAAYTDAAGALVEAAQQ
                     HRTRCPRHSVDAALIEECNAGGAELLDRYAALERQRADLAGRQQALNQRIDRHNADRL
                     AWQADKRENGPRIDRNESDANRWVGSARGFMLSDDFTALARQAGAPAPCTGMRLSEGT
                     AYHGTQGLKSLHACLHAVRGALR"
     gene            complement(983..2050)
                     /locus_tag="VD791_09260"
     CDS             complement(983..2050)
                     /locus_tag="VD791_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08205.1"
                     /translation="MKLRGEVRHVSRQGFARHVDAVEALDQQALAENVWREILQKSFS
                     SIGRSRQDSAIIAPFARMAPRGGLQRAGNAGKMLRARCVARSAAGSAAGSAARSAARS
                     AARSSWGVHVIDLFSLHRNRMPVGVFALALAVTQPGLGRADEEGGRLKLRALIEEGDL
                     ILEEARGLQPAADQLAAEGARLEAEGTALHAESTGLNETIVKFNATNIELQRQVKAHQ
                     AECPPESEDKALVESCNARARELQPTVSVHEEQRPLLQQRQHALQQRIEAHNAARSDW
                     AARKRDLDPRGQANRKDADYWLGEARAFLAGDGFRALAKKAGAPAACAPRTLGDLASP
                     PASAAVERAHACLKAVAGALR"
     gene            2024..2287
                     /locus_tag="VD791_09265"
     CDS             2024..2287
                     /locus_tag="VD791_09265"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HXV08206.1"
                     /translation="MADLAAQLHLEAYGEPVIYAPAGRGKAQNQGFDAFLPLIDSGIA
                     AYVWSSARFFSIVVYTCKRFDEAEAVRFTREFFGAAEVESMAF"
     regulatory      2571..2663
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2816..3232
                     /locus_tag="VD791_09270"
     CDS             2816..3232
                     /locus_tag="VD791_09270"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXV08207.1"
                     /translation="MRTQHGLTLPEVLIVITIVGILAGIALPNYQGYVQRSQLSEAFD
                     SLSAHRLRMDQAFHNNGNFGVGACAVVAPTATVNFTFTCDLTDAGQGYLSRADGIGRM
                     AGFAFSVDDDGRRITTAFPGQATLPANCWMSKKGEC"
     gene            3234..3797
                     /locus_tag="VD791_09275"
     CDS             3234..3797
                     /locus_tag="VD791_09275"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HXV08208.1"
                     /translation="MAACLERSYLQRAATDRSAPRSDGFSMIELMLAVLILSLVLAKA
                     VPSVMDYIRNDRIRSVAEEMRDGLHTARMEAIRRNTTVNFVPNATGWSVVAPGVGGAP
                     DEILHERAARAAELALAAAPSSANVAFNGSGRLTAAGVFTVDVTSSATPCQADGGEAR
                     CLRLNVASGGMIRMCDPALPAGNPQAC"
     gene            3807..4232
                     /locus_tag="VD791_09280"
     CDS             3807..4232
                     /locus_tag="VD791_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08209.1"
                     /translation="MRRFQSGSAMVEGLTAVTVFSIGILGVIGLQASVLENNAQAQFR
                     AEASYLAEEVIGLATADAANAACYAMNPPSPDCANAFAQEAALEWAERVQASLPGAEE
                     ILPQVAYAADGTFTVTIQWKRQSDATWNNYVSTTSISGS"
     gene            4235..5296
                     /locus_tag="VD791_09285"
     CDS             4235..5296
                     /locus_tag="VD791_09285"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HXV08210.1"
                     /translation="MNARRQSGISIVEMMVGLMVGMLVTLSAWGTVMFYEANRRTGVG
                     GNSALENGLATALAIQRDVKSAGLGFVFAGVPACATLNVYHNGATTADGTALAPVAIT
                     DGGAGSDQVSVAYSGSILGGAPVRTIAGMAAPNDLIKVNTTGNILTGNLVLVASASGA
                     DPCTLMQVTNATASGFGTEITRAAGEWNPTDPGTAFSNAPSYPANSNVIRADGFTWVT
                     YRVTNGALEVVDNLTGAVDRLAENVVLLKAQYGTSNGTTPQIEQWVNATDAWAPPLDA
                     THVAAVRAIRIVVVARSPHRERPTVADGPCDATPVAPDSYPGGPAIDLTADPDWQCYR
                     YKTLLLTIPLKNVIFGGQA"
     gene            5293..5817
                     /locus_tag="VD791_09290"
     CDS             5293..5817
                     /locus_tag="VD791_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08211.1"
                     /translation="MKRQQGASLIIVVILLVILMVSALALVRSSETVGAVAGNVAFKQ
                     AATEAGDVGITAATNWLGALTNVDAAVAGVYYATRQNVDGYGLPTVDWTPVPEATVNA
                     FKVQYVVERLCQGPTPVTNLTAQCLTGQSQQAGSKKVGSPSYSGGTAVYYRVTVRVKG
                     PRNSESFVQSLLSK"
     gene            5855..9388
                     /locus_tag="VD791_09295"
     CDS             5855..9388
                     /locus_tag="VD791_09295"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HXV08212.1"
                     /translation="MNTPNKLALGLAALQMCLGAALAAPTDISQLPMDAKSQATPNLI
                     FGVDDSGSMDSEILLNNNDGALWWDSTPRTFWNAAGVTHFNPNGVAGASGGTTWLKYV
                     YLFPNGTGSGNRTYSDATYDHYAVAPTPAYVFLRSSNYNPLYYNPSVNYKPWQPAYIA
                     GATRTFPNAAGNAARSHPWYPTSGVPTTMDLSQTLTSTATNWTFRMVPGMVIPGATIS
                     GIQGRRNGGAYAAVTTDYTIPGGETWDVAIPYLPATYWVKDASCTGTWPTCATAPDGA
                     KLRRYEIRPGVTFPSGRGYLAELQNFANWYTYYRKRRLMLSAGMGDALAAVKGLRGGS
                     VYFNNHQPVTMYDFSAAGDNANARALLGAIHTNTANGGTPTRETLKYIGEQYRTNASI
                     IQYGCQRNAAFILTDGFANASAVTVPAYSQATWVGSPPYQTIYAGSLADLASCYYTTN
                     LRTDLPTGLLNVDPSDTAPNADKNPDLHMNTFGVTLGLTGTIYGTNTPAANNPYLNYP
                     NWPAPTQNRHPSSIDDLWHATINGRGKMFLANNATDLTTHLREVVNSLLTKAGSDAGI
                     AVSNVNLKNGDNTAYVSSYNAQNWTGQLAAFPVNVDTGQVDMSPEAQLWEARDQLTAR
                     DLVADPRFIATYDGAQGVPFQPGAMPPAMEALLATPSASPSDSADVISYLRGDRSLEG
                     VRYRIRAALLGDIVSAEPVFVGGPIATYVDAGYDSFSTSIASRQKMIYQAANDGLVHA
                     FNASNGAELWAYVPAAAYPRLNTLTDPLYSHQFSVDGTPTTGDADLANTVSGGRTTGD
                     WRTLLIGGLGAGGPGVYALDVTQPGAGSEIAAAGKVLWEFPRASTPAGVAANVGLSFG
                     KPVLAKTQAAGWVVLVTSGYNNTAGDGRGHLFVLDARDGQVIADISTGVGSTTDPSGL
                     GQISAWANNAVLDATIDYVYGGDLKGNVWRFDLSGPSTAAWNVVKLATLTDPSSVPQP
                     ITAPPELAEVDGRRMVFVGTGSLLGNSDIANTQVQSMYAMVDNMTSTPLIAAPRTELV
                     NKPVTVGAGGLRNIDPTAVNYAIARGWYFDLPSGGERVNTAPAAAFGVLVFSTNQPSL
                     TACSSQSYLYAVDVAHGGQLPSTAFALGETPWSGKSLGGSLASRPVIVVLPNGSVQSI
                     THKSDTSLTTSRLPVTMGGKVNKVGWKEIFR"
BASE COUNT         1586 a         3447 c         3102 g         1426 t
ORIGIN      
        1 tgaagacgag ggcctgggac cgcagtttgc tcagcggttc atcgaggcgt gggcggaagt
       61 gcacaccaca ccgccccgcc cgctgcgcgg gcaccccgcc tcgtgagagg cggggagcgt
      121 ctttttaaga cgcgccttaa gggcgcattc agcggggtgg cacgcgaagc gtgacggggt
      181 ggtcctgccg tcgtgcgctt ccacctagcg cagcgcaccg cgcacggcgt gcaggcaggc
      241 gtgcagcgac ttcagaccct gggtcccgtg ataggccgtg ccctccgaca ggcgcatgcc
      301 ggtgcagggc gcgggggcgc ccgcctgcct cgcgagcgcc gtgaagtcgt cgctcagcat
      361 gaagccgcgc gccgacccga cccagcggtt cgcgtccgac tcgttgcggt cgatgcgcgg
      421 accgttctcg cgcttgtccg cttgccatgc caggcggtcg gcgttgtggc gatcgatgcg
      481 ctggttcagg gcctgctggc gcccggcaag atcggcgcgc tgccgctcca gagcagcgta
      541 acggtccagg agctcggcgc cgccggcatt gcactcttcg atcagcgcgg cgtcgaccga
      601 gtgtcgcggg cagcgtgtgc gatgctgctg tgccgcctcg acgagcgcgc cggcggcatc
      661 ggtgtaggcc gcaatctcgc gctccagcgc gagcgactcg ctgcgcagcg ccgcctccga
      721 atccgccagg cgctcgcctt cggtgcgcaa gcgatcgatc gtaggctgca ggcccgcgag
      781 ttcctcctgc agcagctcgg cttcctctgc gagcgccgcc agccgcagcc gtgcggcgtc
      841 gtctgcgctg gccggcacgc cgagcgacgc ggtgcaaaca accgccagca cggccaggcg
      901 ccgcttcaca gtgcgatgga ctgacggcat cacggactcc gcaaaaaccc ggcggcaccg
      961 gctcatccgg gcagcgcggc gcctaccgga gcgcgccggc caccgccttc aagcaggcgt
     1021 gcgcccgctc gactgcagcg ctggccggtg gcgaggccag gtcgccgagt gtgcgcggcg
     1081 cgcaggccgc cggagcgccc gccttcttcg ccaatgcccg gaacccgtca ccggcgagga
     1141 acgcgcgcgc ctctcccaac cagtaatcgg catctttgcg attggcttga cctctgggat
     1201 cgaggtcgcg cttgcgcgcc gcccagtcgc tgcgcgcggc gttgtgcgcc tcgatgcgtt
     1261 gctgcagcgc gtgctggcgc tgctgcagca gcggccgctg ctcctcgtgc acactcacgg
     1321 tcggctgcag ctctcgggcc cgcgcattgc aggactcgac cagcgccttg tcttccgact
     1381 cgggcgggca ctcggcctga tgcgccttga cctggcgctg cagctcgatg ttagttgcat
     1441 tgaacttgac gatggtctcg ttcaagccgg tggactcggc gtgcagcgcc gtcccctcgg
     1501 cttcgaggcg ggcgccttcg gcggcgagct ggtcggccgc gggctgcagc ccgcgcgcct
     1561 cttccaggat caggtcgccc tcttcgatca gcgctctgag cttgaggcgg ccgccttctt
     1621 cgtccgcgcg gccgaggccg ggctgcgtga cggccagcgc cagggcgaat acgcccaccg
     1681 gcatgcgatt gcgatgcaat gagaaaaggt cgatcacatg caccccccaa gaggaacgag
     1741 cggcggaacg agcggcggaa cgagcggcgg aaccagcagc ggaaccagca gcggaacgag
     1801 cgacgcagcg agcccgcagc atcttgccag cattgccagc gcgctgcagg ccgccgcgcg
     1861 gagccatgcg cgcgaacggt gcgataatcg cgctgtcctg ccgactcctg ccgatgctgg
     1921 agaagctctt ctgaagaatc tcgcgccaca cgttttccgc cagcgcctgc tgatcgaggg
     1981 cctctacggc atcgacgtga cgcgcgaagc cctggcgcga tacatggcgg acctcgccgc
     2041 gcagcttcat ctcgaagcct acggggagcc ggtgatctac gcgccggcgg gacggggaaa
     2101 ggcgcagaac cagggcttcg acgccttcct gccgctgatc gattcgggga tcgccgccta
     2161 cgtgtggtcc tcggcgcgct tcttctccat cgtcgtgtac acctgcaagc gcttcgacga
     2221 agcggaagcg gtgcgtttca cgcgcgagtt ctttggcgca gccgaagtgg aatcgatggc
     2281 gttctagagg cgagagcact gtctttgctt cactcggcac gacggtgcgc tgcgcatcgt
     2341 catcccctgt cgccctattg cgacactcgc gccgcgatca ccgacccatc ggccgcgttt
     2401 caacctctcg ttccttaaag cacaccgctc atcggccgta attgccttta gcgggtagtg
     2461 tcgtggtagg gtatttcccg tgtggcggca ccggtcgtac gccgcaacgg aggaagacgc
     2521 cagcaatcat ctgcacacca gcatctcacg atccgccccg cacgggtggc tcgtgaaaag
     2581 ggcaaacccg tcgaaaggcg gggacgcaaa gtcaccggcc taccggcctc gcgagcgagg
     2641 cccagggcag cggaaccgcc gtgcgcggca gcgagccgcg acgtaaatcg atggaactgt
     2701 cccgttccgt ccgcgtttcc gtccctgtct ttgccccccc aaccctcgga ccccgcgatg
     2761 ccggcgtgcg tcgtctggcg cttgggaatc gagcaggaac agaggagtgt tcgcaatgag
     2821 gacacaacac ggcctgaccc tgccggaggt gctgatcgtc atcaccatcg tcggcatcct
     2881 cgcgggcatc gccctcccga actaccaggg ctacgtccag cgcagccagc tgagcgaggc
     2941 cttcgactcc ctgtccgcgc atcgcctgcg catggatcag gcgttccaca acaacggcaa
     3001 cttcggcgtg ggcgcctgtg cggtggtcgc gcccactgcc actgtgaact tcaccttcac
     3061 ctgcgacctc accgacgccg gccagggcta tctctcacgc gccgacggca tcggccgcat
     3121 ggcgggcttc gcgttcagcg tggacgacga cggccggcgc atcaccaccg cgtttcccgg
     3181 gcaagccacc ttgcccgcca actgctggat gagcaagaag ggagagtgct gacatggctg
     3241 cgtgcctcga aagatcgtac ttgcagcgcg ccgcgacgga tcgctccgcg ccgcgatcgg
     3301 acggcttctc catgatcgag ctgatgctgg cggtgctgat cctgagcctg gtgctcgcca
     3361 aagccgtgcc ctcggtgatg gactacatcc gcaacgaccg catccgctcc gtggccgagg
     3421 agatgcgcga cgggctgcac accgcgcgca tggaagcgat ccgccgcaac accaccgtca
     3481 acttcgtgcc caacgccacc ggctggtcgg tggtggcgcc cggcgtgggc ggcgcccccg
     3541 acgagatcct gcacgagcgc gcggccaggg ccgcagagct ggctctcgcc gccgcgcctt
     3601 ccagcgcgaa cgtggcgttc aacggcagcg gcaggttgac cgcggccggc gtgttcaccg
     3661 tggacgtcac aagcagcgcg accccgtgtc aggccgacgg cggcgaggcc cgctgcctgc
     3721 ggctgaacgt ggcttcgggc ggaatgatca ggatgtgcga cccggcgttg cccgcgggca
     3781 acccacaagc gtgttaggag gcagtcatgc gtagattcca atccggttcg gcgatggtcg
     3841 agggactcac cgcggtgacc gtgttctcga tcggcatcct gggcgtcatc ggcctgcagg
     3901 cgagcgtgct ggagaacaac gcgcaggcgc agttccgcgc cgaagcgagc tacctggccg
     3961 aggaggtgat cggcctggcc acggcggacg cggccaatgc cgcctgctac gcgatgaatc
     4021 cgccctcgcc cgactgcgcc aacgcgttcg cccaggaggc cgcgctggaa tgggccgagc
     4081 gcgtgcaggc ttcgctgccc ggcgccgagg agatcctgcc gcaggtggcc tacgccgcgg
     4141 acggcacttt caccgtcacc attcagtgga agcgccagag cgacgcgacc tggaacaact
     4201 acgtgtccac caccagcatc tcggggtcgt gaccgtgaac gcgcgcagac aatccggcat
     4261 ctcgatcgtc gaaatgatgg tcgggctgat ggtggggatg ctcgtgaccc tgtcggcctg
     4321 gggcacggtg atgttctacg aagccaatcg ccgcaccggc gtgggcggca acagcgcgct
     4381 ggagaacggg ctcgccaccg cgctcgccat ccagcgcgac gtcaagtccg ccgggctcgg
     4441 cttcgtgttc gccggcgtgc ccgcgtgcgc cacgctgaac gtgtaccaca acggcgccac
     4501 caccgcggac ggcaccgcgc tcgccccggt cgccatcacc gacggcggtg ccggctccga
     4561 ccaggtgtcg gtggcctatt ccggatcgat cctgggcggc gccccggtgc gcaccatcgc
     4621 cggcatggcc gcaccgaacg acctcatcaa ggtcaacacc accggcaaca tcctcaccgg
     4681 caacctggtg ctggtggcca gcgcctccgg cgccgacccc tgcacgctga tgcaggtgac
     4741 caacgccacc gcgtctggat tcggaaccga gatcacgcgc gccgccggcg aatggaaccc
     4801 gaccgacccc ggcaccgcct tcagcaacgc gccgtcgtat ccggccaaca gcaacgtgat
     4861 ccgggccgac ggcttcacct gggtgaccta tcgcgtcacc aacggcgctc tcgaggtggt
     4921 cgacaacctg accggcgcgg tggatcggct ggcggagaac gtggtgttgc tcaaagcgca
     4981 atacggcacc agcaacggca ccacgccgca gatcgagcag tgggtgaacg ccaccgacgc
     5041 ttgggcgccg ccgctggacg ccacgcacgt ggcggcggtg cgcgcgatcc gcatcgtggt
     5101 ggtggcgcgc tcgccgcacc gcgagcgccc gacggtggcc gacggcccgt gcgacgccac
     5161 cccggtagcg cccgacagct atcccggcgg cccggccatc gacctgaccg ccgaccccga
     5221 ttggcagtgc taccgctaca agacgctgct gctgacgatt ccgctcaaga acgtgatctt
     5281 cggaggccag gcgtgaagcg ccagcaaggg gcatcgctga tcatcgtggt catcctgctg
     5341 gtgatcctga tggtgtcagc actggcgctg gtgcgctcct ccgaaaccgt gggcgcggtg
     5401 gccggcaacg tggcgttcaa acaggcggcc accgaagccg gcgacgtcgg catcaccgcc
     5461 gcgaccaact ggctgggggc gctcaccaac gtcgacgcgg cggtggccgg cgtgtactac
     5521 gccacgcgcc agaacgtgga cggctacggc ctgcccaccg tcgactggac cccggttccc
     5581 gaggccaccg tcaacgcctt caaggtgcag tacgtggtcg agcgcctgtg ccagggcccc
     5641 acgccggtga ccaacctgac cgcgcagtgc ctgaccggcc agagccagca ggccggcagc
     5701 aagaaagtgg gctcgccgtc ctactcgggc ggcaccgccg tctactaccg agtgacggtg
     5761 cgggtgaagg gcccacgcaa cagcgagagc ttcgtgcaat cgctgctgag caagtgattc
     5821 tggacccagt gatcctgcac gaggagcagc caccgtgaac acgccgaaca agcttgccct
     5881 ggggcttgcc gccctgcaga tgtgcctggg ggcggcgctc gcggcgccca ccgacatttc
     5941 gcagctgccg atggacgcca agagccaggc caccccgaac ctgatcttcg gcgtggacga
     6001 ctccgggtcg atggattcgg agatcctgct caacaacaac gacggcgcgc tctggtggga
     6061 cagcaccccg cgcaccttct ggaacgcggc cggcgtgacg cacttcaacc ccaatggtgt
     6121 ggccggcgcg agcggcggca ccacttggct gaagtacgtc tacctgttcc cgaacggcac
     6181 cggatcgggt aaccgcacct actcggacgc gacctacgat cactacgccg tggcgccgac
     6241 gccggcctac gtgttcctgc gcagctcgaa ctacaacccg ctgtactaca accccagcgt
     6301 caactacaag ccgtggcagc cggcctacat cgccggcgcg acgcgcacct tcccgaacgc
     6361 ggcgggcaat gcggcgcgca gccacccgtg gtatcccacc agcggggtac cgaccacgat
     6421 ggacctgtcg cagacgctca ccagcaccgc caccaactgg accttccgca tggtgcccgg
     6481 catggtcatc cccggcgcga ccatctccgg catccagggg cgcaggaacg gcggcgccta
     6541 cgcggctgtc accaccgact acaccatccc cggcggcgag acatgggacg tcgcgattcc
     6601 ttatctgccg gcgacctact gggtcaagga tgccagctgc acgggcacct ggccgacctg
     6661 cgccaccgcg cccgacggcg ccaagctgcg ccgctacgag atcaggccgg gcgtcacctt
     6721 cccctcgggg cgcggttatc tcgccgagtt gcagaacttc gccaattggt acacctacta
     6781 ccgcaagcgc cggctgatgc tctccgccgg catgggggac gcgctggccg cggtgaaagg
     6841 gctgcgcggc ggcagcgtgt acttcaacaa ccaccagccg gtcacgatgt acgacttcag
     6901 cgccgccggc gacaacgcca atgcgcgagc gctgctgggc gcgatccaca ccaacaccgc
     6961 caacggcggc acgcccacgc gcgagaccct gaaatacatc ggcgagcagt accggaccaa
     7021 cgcctcgatc atccagtacg gctgccagcg caacgcggcg ttcatcctga ccgacggttt
     7081 cgccaacgcg agcgctgtca cggtaccggc gtacagccag gccacctggg tcggcagccc
     7141 gccctaccag accatctacg ccggcagcct ggcggacctg gcgtcctgct actacaccac
     7201 caacttgcgc accgacctgc ccaccggcct gctcaacgtc gatccgtcgg acacggcgcc
     7261 caatgccgac aagaaccccg acttgcacat gaacaccttc ggggtgacgc tggggctgac
     7321 cggcaccatc tacggcacca acacgccggc ggccaacaac ccgtacctga actatccgaa
     7381 ctggcccgcg cccacgcaaa accgccaccc cagctcgatc gacgatctgt ggcacgccac
     7441 catcaacggc cgcggcaaga tgttcctggc caacaacgcc accgacctga ccacgcatct
     7501 gcgcgaagtg gtcaacagcc tgctcaccaa ggccggctcg gacgccggca tcgcggtgtc
     7561 caacgtcaat ctgaagaacg gcgacaacac cgcgtacgtc tccagctaca acgcgcagaa
     7621 ctggaccggc cagctggccg ccttcccggt gaacgtggac accggccagg tggacatgtc
     7681 gccggaggcg cagctctggg aagcgcgcga tcagctcacc gcgcgcgacc tggtggccga
     7741 cccgcgcttc atcgccacct acgacggcgc gcagggcgtg ccgttccagc cgggtgcgat
     7801 gccgcccgcg atggaggcgt tgctcgccac gccctccgcc agcccgtcgg attcggcgga
     7861 cgtgatcagc tacctgcgcg gcgaccgctc tctggagggc gtccgctacc gcatccgcgc
     7921 ggccctgctc ggcgacatcg tcagcgccga gccggtgttc gtcggcggcc ccatcgcgac
     7981 gtatgtggac gccggctacg acagcttctc caccagcatc gcatcgcgcc agaagatgat
     8041 ctaccaggcc gccaacgacg gcctggtgca tgcgttcaat gcgagcaacg gcgccgagct
     8101 gtgggcctac gtgccagccg cggcctaccc gcgcctcaac acgctcaccg acccgctgta
     8161 cagccaccag ttcagcgtcg acggcacccc caccacgggc gacgccgatc tcgccaacac
     8221 cgtctccggc ggtcgcacta ccggcgactg gcgcacgctg ctgatcggcg gcctgggcgc
     8281 cggcggcccc ggtgtctacg cgctggacgt gacgcagccg ggcgccggca gcgagatcgc
     8341 cgccgccggc aaggtgctgt gggaattccc gcgggcctcc actcctgcgg gcgtggccgc
     8401 caacgtcggc ctgtccttcg gcaagccggt gctcgccaag acgcaggccg cgggctgggt
     8461 ggtactggtc acctccgggt acaacaacac ggctggagac gggcgcggtc atctgttcgt
     8521 gctggacgcg cgcgacggcc aggtcatcgc cgacatctcc accggagtgg gctcgaccac
     8581 cgatccctcc gggctggggc agatctccgc atgggccaac aacgccgtgc tcgacgccac
     8641 gatcgactac gtctacggcg gcgacctcaa gggtaacgtg tggcgcttcg acctgtcagg
     8701 gcccagcact gcggcctgga atgtggtcaa gctggccacg ctcaccgatc cctcgagtgt
     8761 gccacagccg atcaccgcgc cgccggagtt ggcggaagtg gacggccggc gcatggtgtt
     8821 cgtcggcacc ggttccttgc tcggcaacag cgacatcgcc aacactcagg tccagtcgat
     8881 gtacgcgatg gtcgacaaca tgacctcgac tccgctgatc gccgcaccgc gcaccgagct
     8941 ggtcaacaag ccggtcaccg tcggcgccgg cgggttgcgc aacatcgatc cgacggcggt
     9001 gaactacgcc atcgcccgcg gctggtattt cgatctgccg agcggcggcg agcgcgtcaa
     9061 cacggcgccc gcggcggcct tcggcgtgct ggtgttcagc accaaccagc cgtcgctgac
     9121 ggcctgctcg tcgcagagct acctgtacgc ggtcgatgtg gcgcacggcg gccagctccc
     9181 cagcacggct ttcgccctgg gcgagacgcc ttggtccggc aagtcgctcg gcggcagcct
     9241 ggcctcgcgc ccggtgatcg tggtgctgcc caacggcagc gtgcaatcca tcacgcacaa
     9301 gtccgacacc tcgctcacca cctcgcgcct gccggtgacc atgggcggca aggtgaacaa
     9361 agtgggctgg aaagagatct tccgctgatc ggcgcgtgac gcaccggccc gccggcgcac
     9421 ccggcgggcc gtgcgcagca cctcccaacc gtccttgtcg ggttcaccga tgcggcgcac
     9481 atcccatact gtctgatgtg gtacatggca acatggccct cagctcaccg ccctcatagg
     9541 gtgggcacac atgcgatgca t
//
LOCUS       DASPGE010000289         5242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_427881, whole genome shotgun sequence.
ACCESSION   DASPGE010000289 DASPGE010000000
VERSION     DASPGE010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427881"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VD791_09300"
     CDS             <1..485
                     /locus_tag="VD791_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXV08213.1"
                     /translation="DYLSFPQRALFDRLGIRNMVMETDPYGNFLAQGYVLGSGRDWAR
                     LGNLFLQDGVWGAERLLPEGYVDFVSTPAPAWKADDRPIYGGFFWINGDGRFPVPRDA
                     YYMAGAGGQHTLIVPSHDLVVVRLGHYRGQAAGSAALNRALALLMQALPESAGKKPQP
                     "
     gene            complement(508..1959)
                     /locus_tag="VD791_09305"
     CDS             complement(508..1959)
                     /locus_tag="VD791_09305"
                     /inference="COORDINATES: protein motif:HMM:NF045061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08214.1"
                     /translation="MEAVLSKPRKKRKLLVALAVLVVLGVLGGIFAWYKFFREVPQDP
                     FDSPHERFKYGSLGGENEAGIPYWIFVVLPRLFPEYLPGPGGYASLGVAWEEGKELPI
                     GFTRKTVGFPRVSNSCAVCHTAAYRGAENENPTFVPAGPGHTMDVQGYFRFLTRSARD
                     PRFNGPNLMREIELVYDLSWIDALLYRFVIIPVVKKRLLEQEQLFAWMEHPELPAWSR
                     GRDDPMNLTKYFMLRMPEDGTFGPADMPSIWNLNKYTQGMTMNWDGASYDPRTVLTDS
                     ALGIVVEPQDDFEENMQWLEDYLRNLPPPKYPFPVDEKLAAAGLPVFERNCAGCHASE
                     RTGKPVPVEEVGTDRNRLESWNKEAAIAANKTVNRLGVKRTGMVEETLVGYIAVHLDG
                     VWLRAPYLHNGSVPTLRAMLTPPDQRPKVFYRGYDLYDKVDVGFIAQGAEAERVGVRF
                     DTTQRGNGNQGHDFGTRLGAAEKNALIEFLKTM"
     gene            complement(2043..3560)
                     /locus_tag="VD791_09310"
     CDS             complement(2043..3560)
                     /locus_tag="VD791_09310"
                     /inference="COORDINATES: protein motif:HMM:NF045061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08215.1"
                     /translation="METDAERELSSFPGRRGVLRGWCRKVAALLILLLAAVAIYLWAR
                     FGGDRPVVYEDIAEHFKYGSLGGERESGFPYWIWQALPSLCADKLPGEGYRSLGVLYE
                     KDKDLPVGVSKRRVLGMDRVFLNCAVCHTNTVRETPQSEPQLLLGMPANSFDVMGFQS
                     FFFACAQDSRFTPKRVIAEIERIGGEVDIIDRYLVYPLAVFLMQDRIRMLKARFAFAE
                     REAPWGPGRVDTFNFAKAIFNFPMDKIPDKELAGASDFPSIWLQRPRKDAQMQLHWDG
                     NNVKVEERNLSAAFGAGTTPPTIDHAALGRIEEWLLDLAPPSYPFPIDQERAGRGAQV
                     YAAYCVGCHGASGRDFTGERVGQVTPIELIRTDRGRLDSYTYELAANQGQLYTGYEER
                     RFKNFRKTHGYANAPLDGIWLRAPYLHNGSVPTLRDLLEPAARRPKQFHRGYDVYDQA
                     KVGFVSDVPAEGERRYFLYDTRVAGNGNGGHEGKPYGTELPPADKDALVEYMKTF"
     gene            complement(3587..>5242)
                     /locus_tag="VD791_09315"
     CDS             complement(3587..>5242)
                     /locus_tag="VD791_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08216.1"
                     /translation="NEAQGRFEFQVVKDYRAGANPRVHYANRTVCTACHQNGAPIFSR
                     QQWDETNANPRLRARLAAHGAAPYGVGVARGVDEPYAIDNASDRANRLSGYQLLWQAG
                     CGTDDAQAAVCRAAALVLALQVRLSGASQLDRESRRYRSDYAPVIGARWNMLWPEGLH
                     LPDPDLPNRDPRPGLAARVSPAAAHVPAQFDPLLPRAPLETWRAGSPDDLDRLIRGLP
                     EFLAQSDVERLDRALRRSSGAVPMQEANSPCTVTRKRAGARGLRLSFRCGQAHEPARL
                     HGRIYARDATITGGGVEGLSLGVLGALDAADVAGGRLRAGHAQFALKRGNLTARTHRG
                     NAVERVELRWREDGTGTAKVTVREDFARLDAAVAALQRERGRELFAAAPFRRAVIMPA
                     LLGALDAEPGRWCCVEPGAVPPPALERPVREPGRAGGDQALAPFYRYCAVCHDTADPA
                     PPNFLSGAPEEVRAKLAQCAPRIFVRLSMWSVDAAQRAKTPMPPPLALGAHGMGTERW
                     RDDRALSAMLSEAGRWLGAEPGAALRLQDYLERGYENLRPCLN"
BASE COUNT          762 a         1845 c         1802 g          833 t
ORIGIN      
        1 aggactatct gtcgtttccg cagcgcgcgc tgttcgaccg cctcggtatc cgcaacatgg
       61 tgatggagac cgacccctac ggcaatttcc tggcgcaggg ctacgtgctc ggatcgggac
      121 gggactgggc gcgcctggga aatctcttcc tccaggacgg cgtatggggt gcggagcgcc
      181 tgctgccgga aggctatgtc gatttcgtga gcaccccggc tccggcctgg aaagccgacg
      241 acaggccgat ctacggcggc ttcttctgga tcaacggcga cggccgcttc cccgtgccca
      301 gggacgccta ctacatggcg ggggccggcg ggcagcacac gttgatcgtt ccctcgcacg
      361 atctggtcgt ggtgcggctg ggccactacc gcggccaggc ggccggcagc gccgccctga
      421 accgcgcgct cgcgctgctg atgcaggcgc tgccggagtc cgctggcaag aaaccgcagc
      481 cgtagagcgc gccggcgccg atgcgcgcta catggtcttg aggaactcga tcagcgcgtt
      541 cttctccgcc gcgccgaggc gcgtcccgaa gtcgtgaccc tggttgccgt tgccgcgctg
      601 ggtggtgtcg aagcgcacgc ccacccgctc ggcctccgcc ccctgcgcga tgaagccgac
      661 gtcgaccttg tcgtagaggt cgtacccgcg atagaagact ttcgggcgct ggtcgggcgg
      721 cgtgagcatc gcgcgcagcg tcggcaccga gccgttgtgc aggtacggtg cgcgcagcca
      781 cacgccgtcc aggtgcaccg cgatgtaacc gacgagcgtc tcctccacca ttccggtgcg
      841 cttcacaccg aggcgattga cggtcttgtt ggcggcgatg gcggcctcct tgttccagct
      901 ctccaggcgg ttgcggtcgg tgccgacttc ctccaccggt accggtttgc cggtgcgctc
      961 ggatgcgtga cagccggcgc agttgcgctc gaacaccggc aaccccgccg ccgccagctt
     1021 ctcgtccacc gggaacggat acttgggcgg aggcaggttg cgcaggtaat cctccagcca
     1081 ctgcatgttc tcctcgaagt cgtcctgcgg ctcgaccacg atgcccagcg ccgagtcggt
     1141 gagcacggtg cgcgggtcgt agctcgcgcc gtcccagttc atggtcatgc cctgcgtgta
     1201 cttgttcagg ttccagatcg agggcatgtc ggccgggccg aaagtgccgt cctccggcat
     1261 gcgcagcatg aagtacttgg tcaggttcat cgggtcgtcg cggccgcgcg accaggccgg
     1321 cagctcgggg tgctccatcc acgcgaacag ttgctcctgc tcgagcagcc gcttcttcac
     1381 caccgggatg atgacgaagc ggtacagcag cgcgtcgatc caggacaggt cgtacaccag
     1441 ctcgatctcg cgcatcaggt tcggaccgtt gaagcgcggg tccctggcgc tgcgcgtcag
     1501 gaagcggaag tagccctgca cgtccatggt atggccgggc ccggcgggaa cgaaggtcgg
     1561 attctcgttc tcggcgccgc gatacgccgc ggtgtggcac acggcgcagc tgttcgacac
     1621 gcgcgggaag ccgacggtct tcctggtgaa gccgatgggc agctccttgc cctcctccca
     1681 ggccacgccc agggaggcgt agccgccggg gccgggcagg tattccggga acaggcgcgg
     1741 caggacgacg aagatccagt acgggatccc cgcctcgttc tcgccgccga gcgagccgta
     1801 cttgaagcgc tcgtgcggcg agtcgaacgg gtcctgcggc acctcgcgga agaacttgta
     1861 ccacgcgaag atgccgccga ggacgccgag cacgaccagc acggcgagcg cgacgagaag
     1921 cttgcgcttc ttcctgggct tgctgagcac tgcttccatg gtgcctccgg ttatcgtcgt
     1981 cttcgcggtc cccctcaccc ccaccccctc cagggaggtt ttctgcgctc cgggcctcgg
     2041 gttcagaacg tcttcatgta ttcgaccagc gcgtccttgt cggcgggcgg cagctcggtg
     2101 ccgtagggtt tgccctcgtg gccgccgttg ccgttgccgg cgacgcgcgt gtcgtagagg
     2161 aaatagcgcc gctccccttc ggcgggcacg tcggacacga agccgacttt cgcctggtcg
     2221 tacacgtcgt agccgcggtg gaactgcttc ggccggcgcg cggcgggctc cagcagatcg
     2281 cgcagggtgg gcaccgagcc gttgtgcagg tagggcgcgc gcagccagat gccgtcgagc
     2341 ggcgcgttcg cgtagccgtg ggtcttgcgg aagttcttga agcggcgctc ctcgtagccg
     2401 gtatagagct ggccctggtt ggccgccagc tcgtaggtgt aggagtcgag acgtccgcgg
     2461 tcggtgcgga tcagctcgat cggcgtcacc tggcccacgc gctcgccggt gaagtcgcgc
     2521 ccgctggcgc cgtggcaccc gacgcagtag gccgcgtaca cctgcgcccc gcggccggcg
     2581 cgctcctggt cgatcgggaa gggatagctc ggcggcgcca ggtcgagcag ccattcctcg
     2641 atgcgtccca gcgcggcgtg atcgatggtg ggcggggtag tgcccgcgcc gaacgcggcg
     2701 ctcaggttgc gctcctcgac cttgacgttg ttgccgtccc aatgcagctg catctgcgcg
     2761 tccttgcgcg gccgctgcag ccagatggac gggaaatcgc tggccccggc aagctccttg
     2821 tccgggatct tgtccatcgg gaagttgaag atcgccttgg cgaagttgaa ggtgtccacg
     2881 cgccccggcc cccagggggc ctcgcgctcg gcgaaggcga agcgcgcctt gagcatgcgg
     2941 atgcggtcct gcatcaggaa caccgccagc ggatacacca ggtagcggtc gatgatgtcc
     3001 acctcgccgc cgatgcgctc gatctcggcg atcacgcgct tgggcgtgaa gcgcgagtcc
     3061 tgcgcgcagg cgaagaagaa gctctggaag cccatcacgt cgaagctgtt ggccggcatg
     3121 ccgagcagca gctgcggctc gctctgcggg gtctcgcgca cggtgttggt gtggcacacc
     3181 gcgcagttca ggaacacgcg gtccataccc agcacgcgcc gcttggatac gcccaccggc
     3241 aggtccttgt ccttctcgta gagcaccccc agcgaccggt agccctcgcc gggcagtttg
     3301 tccgcgcaca ggctgggcag cgcctgccag atccagtacg gaaagcccga ttcgcgctcg
     3361 ccgccgagcg agccgtactt gaagtgctcg gcgatatcct cgtacaccac cggccgatcg
     3421 ccgccgaagc gcgcccacag atagatcgcg accgcggcga gcagcaggat cagcagcgcc
     3481 gcgaccttgc ggcaccagcc acgcagcacg ccgcgccggc ccgggaagga ggaaagctcg
     3541 cgttcggcat ccgtctccat gcgcgcccct ttgttcgttg ttaccttcaa ttcaagcacg
     3601 gccgcagatt ttcgtagccg cgctcgaggt agtcctgcaa ccggagggcc gcgcccggct
     3661 cggcgccgag ccatcgcccg gcctcgctca gcatcgccga caacgcccga tcgtcgcgcc
     3721 agcgctcggt gcccatgccg tgcgcgccca gcgcgagcgg cggcggcatg ggggtcttgg
     3781 cgcgctgcgc agcgtcgacc gaccacatgc tcaggcgcac gaaaatccgc ggcgcacact
     3841 gcgcgagctt ggcgcgcact tcttccggcg cgccggacag gaagttcgga ggcgccggat
     3901 cggcggtatc gtgacacacc gcgcaatagc gatagaaggg cgcgagcgcc tggtcgccgc
     3961 cggcgcggcc cggctcgcgc accgggcgct caagtgccgg cggcgggacg gcgccgggct
     4021 cgacacagca ccagcgcccc ggctccgcgt ccagggcgcc gagcagcgcc ggcatgatca
     4081 ccgccctgcg aaagggcgcg gccgcgaaca gttcgcggcc gcgctcgcgc tgcaacgcgg
     4141 ctaccgcggc atccaggcgg gcgaagtcct cgcgcacggt caccttcgct gtgccggtgc
     4201 cgtcctcgcg ccaacgcagc tcgacccgct ccacggcgtt gcctcgatgc gtgcgcgccg
     4261 tcaggttgcc gcgcttcagc gcgaactgcg cgtgcccggc ccgcagcctg ccgcccgcca
     4321 cgtcggccgc gtccagtgcg cccaggacgc ccagggaaag accttccacg ccgccgccgg
     4381 tgatcgttgc gtcgcgcgca tagatccggc catgcaggcg cgccggttcg tgcgcctgcc
     4441 cgcagcggaa gctcaggcgc aggccgcgcg cccccgcgcg cttgcgagtc acggtacagg
     4501 gcgagttcgc ctcctgcatt ggcactgcgc cgctagagcg ccgtagcgcc cggtccaagc
     4561 gctcgacgtc cgactgcgcc agaaactccg gcagtccgcg gatcaatcgg tcgaggtcgt
     4621 cggggctgcc ggcacgccat gtctccagcg gcgcccgcgg cagcagcgga tcgaactgcg
     4681 cgggcacgtg agcggccgcc ggcgacaccc tggccgccag ccccggccgg ggatcgcgat
     4741 tgggcagatc cgggtcgggc aggtgcaacc cctccggcca gagcatgttc cagcgcgcgc
     4801 cgatgaccgg cgcgtaatcg ctgcggtaac gccggctctc gcggtcgagc tgcgatgcgc
     4861 cggacagccg aacctgcagc gccagcacca gggcggcggc gcggcacacc gccgcctgcg
     4921 cgtcgtcggt tccgcaaccg gcctgccaca gcagctgata gcccgacagc cggttggcgc
     4981 gatcggacgc attgtcgatc gcgtacggct cgtccacgcc gcgcgccacg cccacgccgt
     5041 acggagcggc gccgtgcgcc gccagccgcg cccgcaggcg cgggttggcg ttggtttcgt
     5101 cccattgctg ccgcgagaag atgggcgcgc cgttctggtg gcaggcggtg cacaccgtgc
     5161 ggttggcgta atgcacgcgc gggttcgcgc ccgcgcgata gtccttcacc acctggaact
     5221 cgaaacgtcc ttgcgcttcg tt
//
LOCUS       DASPGE010000290        12852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1011859, whole genome shotgun sequence.
ACCESSION   DASPGE010000290 DASPGE010000000
VERSION     DASPGE010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12852
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011859"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2071)
                     /gene="secA"
                     /locus_tag="VD791_09320"
     CDS             complement(<1..2071)
                     /gene="secA"
                     /locus_tag="VD791_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HXV08217.1"
                     /translation="MITNLLRKIFGSRNERLLKRYAHTVRAINALEPQIEKLSDAELR
                     ARTDVLRQRHGDGETLDQLLPEAFAVVREAGKRTLNMRHFDVQLIGGIVLHHGKIAEM
                     RTGEGKTLVATLPVYLNALAGQGVHLVTVNDYLASRDAAWMGKIYSALGLTVGVNLSQ
                     MDHASKQRAYAADVTYGTNNEFGFDYLRDNMVYQTEDRVQRPLSYAIVDEVDSILIDE
                     ARTPLIISGQAEDVTELYYKINDLVPKLTRQAEENGPGDYAVDEKAHQILLSEAGHEH
                     AEQIMTAAGLLAEGTSLYDASNITLMHHLHAALRAHALYHRDQHYVVHNGEVVIVDEF
                     TGRLMPGRRWSDGLHQAVEAKEGVRIQRENQTLASITFQNYFRMYRKLAGMTGTADTE
                     AYEFQHIYNLETVVIPTHMDMIREDRMDQVFKTKKEKVQAVIADIRDCYDRGQPVLVG
                     TTSIENSEELAKHLQKEKLPHQVLNAKQHAREAEIVAQAGRPKMVTIATNMAGRGTDI
                     VLGGNPEPEINRLRADESVSEEDKQARIAEVRMRWQDLHNQVVAAGGLHIVGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSLQDHLMRIFASDRVSGLMQKLGMEKGEAIEHPW
                     VTKAIENAQRKVEAHNFDIRKNLLEFDDVANDQRKFVYGWRNQLLEVQDIADTIKPMR
                     EEVVEREI"
     gene            complement(2225..3142)
                     /locus_tag="VD791_09325"
     CDS             complement(2225..3142)
                     /locus_tag="VD791_09325"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXV08218.1"
                     /translation="MNIILVSGGSSRARSIAVGTRQVVIIALLFALTVVALAALMHYF
                     TLRHAVATDAAYLRPLLSSLQAQESARTHAYLRESLNAMASRLGELQARLVRLDALGE
                     RLTKVAGLKPKEFMFDRPPAQGGADSDIPARQLSLGQLGSELDRLAMELEDRTDKLRY
                     LETTLTEQHAKRQLIPSKAPVDGGYYSSGFGWRIDPFSGMNAFHEGLDFMAEPGSPVL
                     AAAGGVVVFAAAHPQYGNMVEIDHGNDLVTRYAHASKIVVKLGDVVLRGSKIAEVGNT
                     GRSTGTHLHFEVRQRGAAQNPVRFLRTPG"
     gene            3234..3767
                     /locus_tag="VD791_09330"
     CDS             3234..3767
                     /locus_tag="VD791_09330"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HXV08219.1"
                     /translation="MQLERVAAMQRTLSRALPGGLAAACRVAYETGGCIVIEAQNGAV
                     AAKVKALSGRLLAALRSRDPALTEIRVEVAVVHRIRPGRARVRRIGPTGLRSLTALAG
                     SLPEGPVQAALRRLLRASNREDQALEDEKRQHHRGHDQGVLEDLPGHAQPAPVLRDQI
                     QDDRRADREHDQEADDA"
     gene            complement(3881..4792)
                     /gene="lpxC"
                     /locus_tag="VD791_09335"
     CDS             complement(3881..4792)
                     /gene="lpxC"
                     /locus_tag="VD791_09335"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HXV08220.1"
                     /translation="MIRQRTLKSTVRATGVGLHTGGKVYLTLRPAAADTGIVFRRIDL
                     PQPVEIKAHATLVGDTRLSSCLEVGTARVYTVEHLMSAFAGLGVDNAYVDLTADEVPI
                     MDGSAGPFVFLIQSAGIEEQDAPKRFIRVLQRVEVRDGDRWARFEPYNGFRMDFSIEF
                     DHPVIQRASQEVSIDFATTSYVKDIARARTFGFMQDVETLRAQGLGLGGSLDNAIVVD
                     DFRVLNPDGLRYDDEFVKHKVLDAIGDLYLLGHPVLGRFAAHKSGHALNNRLSRQLLS
                     ERSAWQFTTFERLEEAPPFVSHDVQYA"
     gene            complement(4888..6042)
                     /gene="ftsZ"
                     /locus_tag="VD791_09340"
     CDS             complement(4888..6042)
                     /gene="ftsZ"
                     /locus_tag="VD791_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HXV08221.1"
                     /translation="MFELVDVQPQEAVIKVVGVGGCGGNAVDHMIEAGVQGVEFICAN
                     TDAQALKRNRASIQLQLGVTVTKGLGAGANPEVGRQAAVEDRERIVELIEGADMLFLT
                     AGMGGGTGTGAAPIVAEVARELGILTVAVVTKPFAFEGKRQRVAQEGLEALSKHVDSL
                     IVIPNEKLMQVLGDDISMLDAFKAANGVLHGAVAGIAEVINCPGLVNVDFADVRTVMS
                     EMGMAMMGSAVGSGPHRSTQAAEQAVASPLLEDVNLAGARGVLVNITASASLKMKEVH
                     DVMNTIKAFTAEDATVIVGTVIDENMGERLRVTMVATGLGSPVARAQPKPLTVVKTGT
                     DVAPIEVVDYDQLDTPAVIRRRNRESTIEAMRQSGVDLLDIPAFLRKQAD"
     gene            complement(6082..7320)
                     /gene="ftsA"
                     /locus_tag="VD791_09345"
     CDS             complement(6082..7320)
                     /gene="ftsA"
                     /locus_tag="VD791_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HXV08222.1"
                     /translation="MNKPKDTENLIVGLDIGTSKIVAMVAEIRPEGGFDVIGYGSYPS
                     RGLKKGVVVNIESTVNAIQRALEEAELMADCKIREVYTGIAGSHIRSFNSHGMVAIKD
                     REVSHLDMDRVIETAKAVNIPTDQQILHILNQEFIIDGQEDVREPLGMSGVRLEVKVH
                     IVTGAVSAAQNIMKCVRRCGLEVDDLVLQPLASAIAVLSEDEKDLGVCLADIGGGTTD
                     IALFTEGAIRHTAVIPIAGDQITSDIAMALRTPTKDAEEIKQKFGCALRELADASETV
                     RVPGVGDRVSRQMSRQTLAEVIEPRVEELYSLIQAELRRSGFEELLSSGIVLTGGSSL
                     MQGMVELGEEIFHMPVRVGTPSYSGSLAEVVRNPRYSTAMGLLMSGVAHYQREQIVRL
                     QSASFKHVIGRMRTWFQGNF"
     gene            complement(7333..8052)
                     /locus_tag="VD791_09350"
     CDS             complement(7333..8052)
                     /locus_tag="VD791_09350"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HXV08223.1"
                     /translation="MWDDHRQLNRLAALLYTLAALGIAYAALMTVIRLPVFPLREVTV
                     AGQILHTTPEQLDAIANRQLRGNFFTLDLEAARRSFEKLPWVRNARLRRAWPDRLEVV
                     LEEHEALARWRDVGLVNTYGEVFDAASASRLPVFAGPDDAAGEIALHYVAFRDALAAI
                     GRSPVEVRLSGRRAWQIKLDDGRLLELGRQDVVRRLVRFAEVYPRVAEQLRDTVYRVD
                     LRYPNGFAVRLPGLRWDERPV"
     gene            complement(8042..8962)
                     /locus_tag="VD791_09355"
     CDS             complement(8042..8962)
                     /locus_tag="VD791_09355"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HXV08224.1"
                     /translation="MSTPREFGKVAVLLGGRSAEREISLMSGNAVLQALREAGVDAHP
                     FDPAEREVFELAREGFQRVFIALHGRYGEDGTVQGALELMGIPYTGSGVMASALAMDK
                     VRTKMVWVACGLPTPRWEILDAGCDWGAVAAGLGLPLIVKPVHEGSTLGLTKVTRADE
                     LPAAYALAARYDRLVLAEEFVAGSELTAGFVGDQALPLIRIEAPEGKYDYQHKYFSDE
                     TRYFCPCGLPDAEEQAIRRLTLRAADALGCSGWGRADLIRRTDGSVSLLEMNTSPGMT
                     GHSLVPMAAKVAGMSFQQLVLRILELAHVG"
     gene            complement(8959..9918)
                     /gene="murB"
                     /locus_tag="VD791_09360"
     CDS             complement(8959..9918)
                     /gene="murB"
                     /locus_tag="VD791_09360"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HXV08225.1"
                     /translation="MDMAERRHLAAAPAGELRRDEPMHKHTSWRAGGPAQRAYMPRDL
                     LDLAAFLRGLPADEPVHPVGLGSNLLVRDGGVRGTVLFTHGALKEVRLEPGPDGAPLV
                     YAQAGVASPKVARFAATHDLRGAEFLTGIPGTVGGALAMNAGCYGSETWEIVDSVLVV
                     THAGELKRRIPAEYEIGYRHVRLRPDASQSRITNHESQECFAAAWLRLARGDGEEGRR
                     VIRELLQRRIASQPLDQPNAGSVFRNPPGDYAARLIESCGLKGRSIGGAMVSPKHANF
                     IVNTGTASAADIEALIELVQQTVQARCGVLLEREVRIIGEKAQ"
     gene            complement(9905..11329)
                     /gene="murC"
                     /locus_tag="VD791_09365"
     CDS             complement(9905..11329)
                     /gene="murC"
                     /locus_tag="VD791_09365"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HXV08226.1"
                     /translation="MKHKVKRVHFVGIGGSGMSGIAEVLLTLGYQVSGSDLSESAVTE
                     RLRGLGARIHRGHDPALVQGADVVVVSTAVKPDNPEVGAARARRIPVVPRALMLAELM
                     RLRQGIAIAGTHGKTTTTSLIASVLAEAGMDPTFVIGGRLEAAGTHARLGRGEFIAVE
                     ADESDASFLHLQPVLAVVTNIDADHMETYGHDFGRLRQAFLDFLQRLPFYGMAVLCVD
                     DANVRAIMPLVTKPITTYGLSEQAQIRATDIRHHSGRMRFTAEIRANGGPGKSLDVVL
                     NLPGVHNVRNALAALAVGLEVQAPEEAILRALAEFKGVGRRFQRYGDIALPAGGSFAL
                     IDDYGHHPAEMEATLAAVRGAFPGRRLVLAFQPHRYTRTRDCFEDFVRVLSGVDALVL
                     TEVYAAGEVPIVAADGRALARALRVAGKVEPVFIEQIADMAQAILAAARDGDVVLTMG
                     AGSIGGVPGQLARQQKQATANGHG"
     gene            complement(11329..11472)
                     /locus_tag="VD791_09370"
     CDS             complement(11329..11472)
                     /locus_tag="VD791_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08227.1"
                     /translation="MKLKPKAVKRAANPAPLRAPAPASVARKARGQDLGDRRLQLLLG
                     AVR"
     gene            complement(11499..12569)
                     /gene="murG"
                     /locus_tag="VD791_09375"
     CDS             complement(11499..12569)
                     /gene="murG"
                     /locus_tag="VD791_09375"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HXV08228.1"
                     /translation="MSVAVRTILIMAGGTGGHVFPGLAVADEMRSAGWRVVWLGGKDG
                     MEVDLVPRRGYQLEVIRFTGLRGKGWLRMALLPLNLLIAFWQSARVLFRVRPDVVLGM
                     GGYVSFPGGMMAALLNRPLVVHEQNSIAGLANKVLAEVSDQRLSGFPGALRKSQWCGN
                     PVRAEIAALPAPEERFRHRAGPLQLLVVGGSLGARALNEIVPQSLALISAERRPRVTH
                     QSGIKHLERLRDNYALAGVPAETVAFIEDMAAGYGRADLVICRAGALTVTELAAAGVA
                     SILVPFPYAVDDHQTHNARFLADAGAAVLVQQRDLSPRRLADLLLELTRGKLLEMAAR
                     ARALAKPDAARSVAAVCMAAAG"
     gene            complement(12676..>12852)
                     /locus_tag="VD791_09380"
     CDS             complement(12676..>12852)
                     /locus_tag="VD791_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HXV08229.1"
                     /translation="GAQALISMGVNMGVLPTKGLTLPFLSFGGSGLLANCCALALLVR
                     VDFENRQLMRGRSV"
BASE COUNT         1925 a         4530 c         4294 g         2103 t
ORIGIN      
        1 tgatctcgcg ctcgacgacc tcctcccgca tgggcttgat ggtgtcggcg atgtcctgga
       61 cctcgagcag ctggttgcgc cagccgtaga cgaacttgcg ctggtcgttg gcgacgtcgt
      121 cgaactccag caggttcttg cggatgtcga agttgtgcgc ctcgaccttg cgctgggcgt
      181 tctcgatggc cttggtcacc cacgggtgct cgatggcctc gcccttctcc atgcccagct
      241 tctgcatcag gccggagacc cggtcggacg cgaagatgcg catcaggtgg tcctgcagcg
      301 acaggtagaa acggctggag cccgggtcgc cctggcgccc ggaacggccg cgcagctggt
      361 tgtcgatgcg gcgggactcg tggcgctcgg tgccgacgat gtgcagcccc ccggcggcga
      421 ccacctggtt gtgcagatcc tgccagcgca tacgcacctc ggcgatgcgc gcctgcttgt
      481 cctcctccga gacgctctcg tcggcgcgca ggcggttgat ttctggctcc gggttgccgc
      541 ccaggacgat gtcggtgccg cggccggcca tgttggtggc gatggtgacc atcttcggcc
      601 ggcctgcctg cgccacgatc tccgcctcgc gcgcatgctg cttggcgttg agcacctggt
      661 gcggcagctt ctccttctgc agatgcttgg ccagctcctc cgagttctcg atcgaggtgg
      721 tgcccaccag caccggctgg ccgcgatcgt agcaatcccg gatgtcggcg atgaccgcct
      781 gcaccttctc cttcttggtc ttgaacacct ggtccatgcg gtcttcccgg atcatgtcca
      841 tgtgcgtcgg gatgaccacc gtctccaggt tgtagatgtg ctggaactcg taggcctcgg
      901 tgtcggcggt gccggtcatg cccgccagct tgcggtacat gcggaagtag ttctggaacg
      961 tgatcgaggc gagcgtctgg ttctcgcgct ggatgcgcac gccctccttc gcctccaccg
     1021 cctgatgcag cccgtcggac cagcgccgtc cgggcatcag gcgcccggtg aactcgtcca
     1081 cgatcaccac ctcgccgttg tgcaccacgt aatgctgatc acggtgatac agggcgtgcg
     1141 cacgcagcgc cgcgtgcagg tgatgcatga gcgtgatgtt ggacgcgtcg tacagactgg
     1201 tgccctcggc cagcaacccg gccgcggtca tgatctgctc cgcgtgctcg tgcccggctt
     1261 cgctcagcag gatctggtgc gccttctcgt ccaccgcata gtcgcccggg ccgttctcct
     1321 cggcctggcg cgtgagcttg ggcaccaggt cgttgatctt gtagtacagc tccgtgacgt
     1381 cctcggcctg cccggagatg atgagcggcg tgcgcgcctc gtcgatcagg atcgagtcga
     1441 cctcgtcgac gatggcgtag ctcaacgggc gctgcacgcg gtcctcggtc tggtagacca
     1501 tgttgtcgcg caggtagtcg aagccgaact cgttgttggt accgtaggtg acgtcggccg
     1561 cgtacgcgcg ctgcttgctg gcgtggtcca tctgcgacag gttcacgccg acggtcagac
     1621 ccagggcgct gtagatcttg cccatccagg cggcgtcgcg gctggccagg taatcgttca
     1681 ccgtcaccag gtgcacgccc tgaccggcca gcgcattcag gtacaccggc agcgtggcga
     1741 ccagggtctt gccctcgccg gtgcgcatct cggcgatctt gccgtggtgc aggacgatcc
     1801 cgccgatcag ctgcacgtcg aagtggcgca tgttcagcgt gcgcttgccg gcctcgcgca
     1861 ccacggcgaa ggcttcgggc agcagctgat cgagcgtttc gccgtcgccg tgacgctggc
     1921 gcaagacgtc ggtcctggcg cgcagctcgg cgtcggagag cttctcgatc tgcggctcga
     1981 gcgcgttgat ggcgcgcacc gtgtgggcgt agcgcttgag caggcgctcg ttgcggctgc
     2041 cgaagatctt cctgagcagg ttggtaatca tccgtcgtcc agatgtaggc gacgacccgg
     2101 ggatcaagcc ttgccgaagc ccgcgcgcgg gcggcgcagc ggtcgcgccg ccccaaaaaa
     2161 gcacgagggg cgaggggccc gaccagggcc cgtcacccct ctcgcggcga tgacgcttgg
     2221 gagctcagcc gggcgttctg aggaagcgca ccggattctg ggctgcgccc ctctggcgca
     2281 cttcgaagtg caggtgagtc ccggtggaac ggcccgtgtt gccgacttcg gcgatcttgc
     2341 tcccccgcag caccacgtcg cccagcttca cgacgatctt cgaagcgtgc gcgtagcgcg
     2401 tgacgaggtc gttgccgtga tcgatctcga ccatattacc atactgcgga tgagcggccg
     2461 caaacaccac cacgccgccg gcggcggcga gtacgggcga gcccggctcg gccatgaagt
     2521 ccagcccttc gtggaaggcg ttcatcccgc tgaacggatc gatgcgccag ccgaagcccg
     2581 acgagtaata accgccgtcc accggcgcct tgctggggat gagctggcgc ttggcgtgct
     2641 gctcggtgag cgtcgtttcc aggtagcgca gcttgtcggt gcggtcctcg agctccatgg
     2701 cgaggcggtc gagctcgctg cccagctgtc ccagggacag ctggcgagcg gggatgtcgg
     2761 agtcggcgcc gccttgggcg gggggccggt cgaacatgaa ttccttgggc ttgaggccgg
     2821 cgaccttggt caggcgctcg cccagcgcgt ccagccgaac cagccgggcc tgcagttcgc
     2881 cgagccggga ggccatggcg ttgaggctct cgcgcaggta cgcgtgggtg cgggcgctct
     2941 cctgcgcctg caggctggac agcagcgggc gcaggtacgc ggcatcggtg gccaccgcgt
     3001 ggcgcagagt gaagtagtgc atcaatgcgg ccagagccac cacggtgagc gcgaacagca
     3061 gcgcgatgat gacgacctgc cgggttccga cggcgataga ccgcgcccgc gaggaaccgc
     3121 cggaaactag aatgatgttc atcgtttccc ctttgccacc catgccctcg agcgcgccag
     3181 gccggcgcat aggcttgttg ttggactctg tcccagcgct aaaagcaagt agcgtgcaac
     3241 tggagcgcgt tgctgcgatg caacgcacgc tgtcgcgcgc gctccccggc ggcctcgccg
     3301 ccgcctgcag agtcgcctat gagaccgggg gatgtattgt catcgaagcg caaaacgggg
     3361 ccgtcgcggc caaggtcaag gccctgtccg ggcgattgct ggccgcgctg cggagccgcg
     3421 acccggcgct gacggaaatt cgcgtcgagg tcgctgttgt gcaccgcatc aggcccggcc
     3481 gggcacgcgt tcgtcgcatc ggcccgacag gcttgcgcag cctcaccgcc ctggccggat
     3541 cgctgccgga gggaccggtg caagcggcgt tgcgccgcct gctgcgcgct tcaaaccgcg
     3601 aggatcaggc gctcgaggac gagaaacgcc agcaccaccg cggccacgat cagggcgtac
     3661 ttgaagactt gccaggccac gcgcagccag cgccggtcct gcgtgatcag atacaggacg
     3721 accgacgcgc cgatcgcgag cacgaccagg aagccgatga tgcgtaaggt gacagcggtc
     3781 atgagccgtc gcccggcgca ggctcagtga gcacagagcg gagctgaagc ttcacagccg
     3841 tccttgcggg gcgatggagc acggaccccc gccggatagc tcaggcgtac tgaacgtcgt
     3901 ggctgacgaa ggggggcgct tcttccagcc gctcgaaggt ggtgaactgc caggcgctgc
     3961 gctcgctcag cagctgccgc gacagccggt tgttgagcgc gtggccgctc ttgtgcgcgg
     4021 cgaagcggcc gaggacggga tggcccagca ggtataaatc gccgatggcg tcgagcacct
     4081 tgtgcttgac gaattcgtcg tcgtagcgca ggccgtcggg attgagcacg cggaagtcgt
     4141 ccacaacgat ggcattgtcc aggctgcctc ccagccccag cccctgagcg cgcagcgttt
     4201 cgacatcttg catgaagccg aaggtgcgcg cccgcgcgat gtccttgacg taggaggtgg
     4261 tggcgaaatc gatgctgacc tcctggcttg cccgctggat caccgggtgg tcgaactcga
     4321 tgctgaagtc catgcggaaa ccgttgtagg gctcgaagcg ggcccaccgg tcaccgtcgc
     4381 gcacttccac ccgctgcagc acgcggatga atcgcttggg ggcgtcctgc tcctcgatgc
     4441 cggcggactg gatcaggaac acgaacggcc cggcactgcc gtccatgatc ggcacttcgt
     4501 cggcggtgag atcgacgtaa gcgttgtcaa cgcccagccc ggcgaaggcc gacatcaggt
     4561 gctcgacggt gtacacgcgc gctgtgccga cctccaggca ggaagacaaa cgggtgtcgc
     4621 ctaccagcgt cgcgtgcgct ttgatctcga ccggctgcgg cagatcgatg cgccggaaca
     4681 cgataccggt gtccgcggcg gccggccgca gcgtgagata caccttgccg ccggtgtgca
     4741 atcccacacc ggtggcgcgt actgtgctct tgagcgtgcg ctgtctgatc atccaagccc
     4801 cgtggccggg taaacggcta attttacgcg attcctgcgc cgatccgaaa cccgtctgcg
     4861 cgagccgtcc aggcgcgtcc gggcgcctca atccgcctgc ttgcgcaaga acgcgggaat
     4921 gtccagcagg tctacgcccg actggcgcat cgcctcgatg gtcgattccc ggttgcggcg
     4981 gcggatcacc gccggcgtgt cgagctggtc gtagtcgacc acctcgatcg gcgccacatc
     5041 ggtgccggtc ttcaccaccg tcagcggctt gggctgcgcg cgcgccaccg gcgagcccaa
     5101 accggtcgcc accatggtca cccgcagccg ctcgcccatg ttctcatcga tcacggtgcc
     5161 gacgatcacc gtcgcgtcct ccgcggtgaa cgccttgatg gtgttcatta cgtcatgcac
     5221 ctctttcatc ttgaggctgg cgctggcagt gatgttgacc aggacgccgc gcgctccggc
     5281 caggttgacg tcctccagca gcgggctggc caccgcctgc tcggccgcct gagtgctgcg
     5341 gtgtggcccg gagcccacgg ccgagcccat catcgccatg cccatctcgg acatcacggt
     5401 gcgcacgtcg gcgaagtcca cgttcaccag gcccgggcag ttgatgacct cggcgatgcc
     5461 ggcgaccgct ccgtggagca cgccgttggc ggccttgaag gcatccagca tgctgatgtc
     5521 gtcgcccagc acctgcatca gcttctcgtt ggggatcacg atcagcgagt cgacgtgctt
     5581 ggacagcgcc tccagcccct cctgcgccac gcgctggcgc ttgccctcga acgcgaacgg
     5641 cttggtcacc accgccacgg tgaggatgcc cagctcgcgc gccacctcgg ctacgatcgg
     5701 ggcggcgccg gtgccggtgc cgccgcccat gccggcggtc aggaacagca tgtccgcgcc
     5761 ctcgatcagc tcgacgatgc gctcccggtc ctccaccgcc gcctggcgcc cgacctcggg
     5821 gttggcgccc gcacccaggc ccttggtgac ggtgacgccg agctggagct ggatcgaggc
     5881 gcggttgcgc ttgagcgcct gcgcatcggt gttggcgcag atgaactcca ccccctgcac
     5941 gcctgcctcg atcatgtgat ccacggcgtt gccgccgcaa ccgccgactc cgaccacctt
     6001 gatcaccgct tcctgcggct gcacgtcgac cagctcgaac atcgcgtttc ctcctttgtg
     6061 cgttgaatcg aaacagaacc cctagaagtt cccctgaaac catgtccgca tgcgcccgat
     6121 cacgtgcttg aaggaggcgc tctgcagccg gacgatctgt tcgcgctggt agtgcgcgac
     6181 gccggacatg agcagtccca tcgcggtcga gtagcgcgga ttgcgcacca cctccgccag
     6241 gctgccgctg tagctggggg tgcccacgcg caccggcata tggaagatct cctcgcccag
     6301 ctccaccatg ccctgcatca ggctggagcc gccggtcagc acgatgcccg aggacagcag
     6361 ctcctcgaag ccggagcggc gcagctccgc ctggatcagc gaatacagct cctccacccg
     6421 cggttcgatc acttcggcga gcgtctggcg cgacatctgc cgcgagacgc gatcgcccac
     6481 accgggcacc cgtaccgtct cggaggcgtc cgccagctcg cgcaaggcgc agccgaactt
     6541 ctgcttgatc tcctcggcgt ccttggtcgg ggtgcgcagc gccatggcga tgtcgctggt
     6601 gatctggtcg ccggcgatgg ggatcaccgc ggtgtggcgg atcgcgccct cggtgaacag
     6661 cgcgatatcg gtggtgccgc cgccgatgtc ggccaggcac acgcccaggt ccttctcgtc
     6721 ctcggacagc accgcgatgg ccgaggcgag cggctgcagc accaggtcgt ccacctccag
     6781 cccgcagcgg cgcacgcatt tcatgatgtt ctgggcggcg gacacggcgc cggtgacgat
     6841 gtgcaccttc acctccagcc gcacgccgct catgcccagc ggctcgcgca cgtcttcctg
     6901 tccgtcgatg atgaattcct gattcaggat gtgcaggatc tgctggtcgg tggggatgtt
     6961 caccgccttg gcggtctcga tcacgcgatc catgtcgagg tggctcacct cgcggtcctt
     7021 gatcgccacc atgccgtgcg agttgaagct gcggatatgg ctgccggcga tgccggtgta
     7081 gacctcgcgg atcttgcagt cggccatcag ctcggcttcc tcgagcgcgc gctggatggc
     7141 gttgacggtg gattcgatgt tcaccaccac gcccttcttc agcccgcgcg acggatagct
     7201 gccgtagccg atcacgtcga acccgccctc cggcctgatc tcggcgacca tggcgacgat
     7261 cttggacgtg ccgatgtcca gcccgacgat gaggttctcg gtgtctttcg gtttgttcat
     7321 gccatgctcc cttcagaccg gccgctcgtc ccagcgcagg ccgggcaagc gcaccgcaaa
     7381 cccattcggg tagcgcaaat cgacgcggta gacggtgtcg cgcagctgct cggcaacgcg
     7441 cggatagacc tcggcgaaac gcaccaggcg ccgcaccacg tcctggcggc cgagctcgag
     7501 cagccggccg tcgtccagct tgatctgcca ggcgcggcgg cccgacaggc gcacctcgac
     7561 ggggctacgc ccgatcgccg cgagcgcgtc gcggaacgcc acgtaatgca gcgcgatctc
     7621 gccggcggcg tcgtcggggc cggcgaacac cggcagccgc gaagcgctgg ccgcgtcgaa
     7681 cacctcgccg taggtgttca ccagcccgac gtcgcgccag cgcgccagcg cctcgtgctc
     7741 ctccagcacc acctccagcc ggtcgggcca ggcgcgccgc agcctggcgt tgcgcaccca
     7801 gggcagcttc tcgaaggatc gacgcgccgc ctccagatcc agcgtgaaga agttgccgcg
     7861 caactggcgg ttggcgatcg cgtccagctg ctcgggcgtg gtgtgcagga tctggccggc
     7921 cacggtgacc tcgcgcagcg ggaataccgg caggcggatc accgtcatca gcgccgcgta
     7981 ggcgatgccc agcgcggcta gcgtgtagag cagtgccgcg agccggttca actgccggtg
     8041 gtcatcccac atgggccagc tccagaatgc gcagtaccag ctgctgaaag ctcatgccgg
     8101 ccaccttggc cgccatcggc accaggctgt ggccggtcat gcccggggag gtgttcatct
     8161 ccagcagcga cacgctgccg tcggtgcggc ggatcaggtc cgcgcgcccc cacccgctgc
     8221 agcccagcgc gtcggcggcg cgcagcgtca gccggcggat ggcctgctcc tcggcgtcgg
     8281 gcagcccgca cgggcagaag tacctggtct cgtcgctgaa gtacttgtgc tggtagtcgt
     8341 acttgccctc cggcgcctcg atgcggatca gcggcagcgc ctgatcgccc acgaacccgg
     8401 cggtcagctc gctgccggcg acgaactcct ccgccagcac cagccggtcg tagcgcgcgg
     8461 cgagcgcgta ggccgcgggc aactcgtcgg cgcgcgtcac cttggtcagc cccagcgtgg
     8521 agccctcgtg caccggcttc acgatcagcg gcaaccccag gccggctgcc acggcgcccc
     8581 agtcgcaccc ggcatcgagg atttcccagc gcggcgtggg caggccgcag gcgacccaca
     8641 ccatcttcgt gcgcaccttg tccatggcga gcgccgaggc catcaccccg ctgccggtgt
     8701 acgggatccc catcagctcg agcgcgccct gcaccgtgcc gtcctcgcca tagcgcccgt
     8761 gcagcgcgat gaacacgcgc tggaagccct cgcgcgcgag ctcgaacacc tcgcgctctg
     8821 cggggtcgaa cgggtgcgca tccacacccg cctcgcgcag cgcctgcagc accgcgttgc
     8881 ccgacatcag cgagatctcg cgctcggcgg agcggccgcc cagcagcacc gccaccttgc
     8941 cgaactcccg gggtgtgctc attgcgcttt ctccccgatg atgcgcacct cgcgctcgag
     9001 caacacaccg cagcgggcct gcaccgtctg ctgcaccagc tcgatcagcg cctcgatgtc
     9061 ggccgcgctc gccgtcccgg tgttgacgat gaagttcgcg tgcttgggcg acaccatcgc
     9121 gccgccgatg ctgcgcccct tgaggccgca ggactcgatc aggcgcgcgg catagtcgcc
     9181 gggcgggttg cggaacaccg aaccggcgtt gggctggtcc agcggctgac tggcgatgcg
     9241 ccgctgcagc agctccctga tcacgcgcct gccctcctcg ccgtcgccgc gcgccaggcg
     9301 caaccaggcg gcggcaaaac actcttgtga ttcgtgattc gtgattcgtg actggctcgc
     9361 gtccgggcgc aggcgcacgt ggcgatagcc gatctcgtat tccgcaggga tgcgccgctt
     9421 gagctcgccg gcgtgcgtga ccaccagcac gctgtcgacg atctcccacg tttccgagcc
     9481 gtagcaaccg gcgttcatgg cgagcgcgcc gcccaccgtg ccggggatgc cggtcaggaa
     9541 ctcggcgccg cgcagatcat gcgtggcggc gaagcgcgcc accttcggac tcgcgacccc
     9601 ggcctgtgca tacaccagcg gcgcgccgtc cggtcccggc tccaggcgca cttccttgag
     9661 cgcgccgtgt gtgaacagca ccgtgccgcg cacaccgccg tctcgaacca gcagattgct
     9721 gccgagcccg accggatgga ccggctcgtc cgccggcagg ccgcgcagga aggcggcgag
     9781 gtcgagtaga tcgcgcggca tgtaggcacg ctgcgccggc ccgccggcgc gccagctggt
     9841 gtgcttgtgc atcggctcgt cccggcgcag ttcgccggcc ggcgcggccg ccagatgccg
     9901 ccgctcagcc atgtccattt gccgtcgcct gcttctgttg gcgcgccagc tgtcccggca
     9961 cgccgccgat ggagcccgcg cccatggtca gcaccacgtc accgtcgcgc gcagcagcga
    10021 ggatggcctg ggccatgtcg gcgatctgct cgatgaacac cggctccacc ttgccggcca
    10081 cgcgcagcgc gcgcgccagc gcccgcccgt cggcggccac gatcggcact tccccggcgg
    10141 cgtacacctc cgtcagcacc agcgcatcca cgcccgacag cacgcgcacg aagtcctcga
    10201 agcagtcgcg cgtgcgcgtg taacgatgcg gctggaacgc gagcaccagg cgccgcccgg
    10261 gaaatgcacc gcgcaccgcg gcgagcgtgg cctccatctc ggccgggtgg tgaccgtagt
    10321 cgtcgatcag agcgaagctc ccgcccgcgg gcagcgcgat gtcgccgtag cgctggaagc
    10381 ggcgccccac gcccttgaac tccgccaggg cccgcaggat cgcctcttcc ggcgcctgca
    10441 cctcgagccc gaccgcgagc gccgccaggg cgttgcgcac gttgtgcacg ccgggaaggt
    10501 tgagcacgac gtccagcgac ttgcccgggc cgccgttggc gcgaatctcg gcggtgaagc
    10561 gcatgcgccc gctgtggtga cggatgtcgg tggcgcggat ctgcgcctgc tcggacagac
    10621 cgtaggtggt gatcggcttg gtcaccagcg gcatgatcgc gcgcacgttg gcgtcgtcca
    10681 cgcacagcac cgccatgccg tagaacggca ggcgctgcag gaagtcgagg aacgcctggc
    10741 gcagccgccc gaaatcgtgg ccgtaggttt ccatgtggtc ggcgtcgatg ttggtgacca
    10801 ccgccagcac cggctgcagg tgcaggaacg aggcgtccga ttcgtccgct tccaccgcga
    10861 tgaactcgcc gcgcccgagc cgcgcatgcg tgcccgccgc ttccagccgg ccgccgatca
    10921 cgaaggtagg gtccatgccc gcttccgcga gcacgctggc gatcaggctg gtggtggtgg
    10981 tcttgccgtg cgtgccggcg atggcgatgc cctggcgcag gcgcatcagc tctgccagca
    11041 tcagcgcgcg cggcaccacc gggatgcgcc tggcgcgcgc cgcgcccact tcgggattgt
    11101 cgggcttgac cgcggtggat acgaccacca cgtcggcgcc ctgcaccagc gccgggtcgt
    11161 ggccccggtg gatgcgtgcc ccgaggccgc gcagccgctc cgtcacggcg ctttcggaca
    11221 gatccgagcc gctcacctgg tagccgagcg tgagcagcac ttcggcgatg ccgctcatgc
    11281 ccgagccgcc gatgccgacg aagtgcacgc gcttgacctt gtgcttcatc agcgcaccgc
    11341 ccccagcagc agctgcagcc ggcggtcgcc gagatcctgg ccgcgggctt tgcgcgcgac
    11401 gctcgcagga gccggcgcgc gcagcggtgc ggggttggct gcacgcttga cggctttcgg
    11461 cttgagcttc atgggaggct cccgtttcgg tcgatgcatc atccggccgc cgccatgcac
    11521 accgccgcga cgctgcgtgc ggcgtcgggc ttggccagcg cgcgcgcgcg cgcggccatc
    11581 tcgagcagct tgccgcgcgt caactccagg agcagatcgg cgagccggcg gggggacaga
    11641 tcgcgctgct gcaccagcac tgccgcgccg gcatcggcca gaaagcgtgc gttgtgcgtc
    11701 tgatggtcgt ccaccgcata gggaaacggc accaggatgc tcgccacgcc cgcggcggcc
    11761 agttcggtga cggtgagcgc ccccgcgcga cagatcacca ggtcggcgcg gccgtagccc
    11821 gccgccatgt cctcgatgaa agcgacggtc tcggccggca cgccggcgag cgcgtagttg
    11881 tcgcgcaggc gttccaggtg cttgatgccg gattgatggg tcacccgcgg ccggcgctcg
    11941 gcggaaatga gcgccagcga ttgcggcacg atctcgttca gggcccgggc gccgaggctg
    12001 ccgccgacca ccagcagctg cagcgggccg gcgcgatggc ggaaacgttc ctcgggcgcg
    12061 ggcagcgcgg cgatctccgc acgcaccggg ttgccgcacc attgcgactt gcgcagcgcg
    12121 ccgggaaagc cgctcaggcg ctggtcggac acctcggcca gaaccttgtt cgccagcccc
    12181 gcgatcgagt tctgctcgtg cactaccagc ggccggttga ggagcgccgc catcatgccc
    12241 ccgggaaaag acacatagcc gcccatgccg agcaccacgt cggggcgcac gcgaaacagg
    12301 acgcgcgcgc tctgccagaa ggcgatcagc agattgagcg gcagcagcgc catgcgtagc
    12361 caccccttgc cgcgcagacc ggtgaagcgg atcacctcca gctggtaacc ccggcgcggc
    12421 accaggtcga cctccatgcc gtccttgccg cccagccaca ccacgcgcca gccggcgctg
    12481 cgcatctcgt ctgcaacggc tagtcccggg aacacatgac cgccggtgcc cccggccatg
    12541 atcaggatgg tgcgcacggc gacgctcata cgctccgccc gcgcatcggc tgccggtttt
    12601 ggctccggcc gcgccgaagg cgcttaacgt ccgctgcgcg gacgttagcc tccaccgaaa
    12661 ccgtcaggcg agctttcata cgcttcgccc gcgcatcagc tgccggtttt cgaaatccac
    12721 gcgcaccagc agcgccagcg cgcagcagtt ggcgagcagg ccactgccgc cgaagctcag
    12781 gaacggcagg gtgagtccct tggtgggcag cacgcccatg ttcacgccca tgctgatcag
    12841 ggcctgcgcg cc
//
LOCUS       DASPGE010000291         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1363150, whole genome shotgun sequence.
ACCESSION   DASPGE010000291 DASPGE010000000
VERSION     DASPGE010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363150"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VD791_09385"
     CDS             <1..336
                     /locus_tag="VD791_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08230.1"
                     /translation="QRPTFEPEIPVLEEAIEALDEAALQWVTAGDRRRQLQVALYLRL
                     RQRLDREIEALAQGRTQALDARLTRVVQALRDALPAIVRESVDQALGGRSPGVGGQSS
                     VRDDDANDR"
     gene            375..>2066
                     /locus_tag="VD791_09390"
     CDS             375..>2066
                     /locus_tag="VD791_09390"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HXV08231.1"
                     /translation="MELAKSFEPREIERRWYQIWESRGYFSASLASEAPPYCIQLPPP
                     NVTGTLHMGHAFQQTLMDALIRYHRMRGFNTNWVAGTDHAGIATQIVVERQLEAEGKS
                     RHDLGREAFVARVWRWKQQSGAAITRQMRRLGASANWTYADTEGQRAGYFTMDARMSR
                     AVIEVFVRLHEEGLIYRGKRLVNWDPVLGTAVSDLEVDNEEEDGRIWEIRYPAPGDGT
                     GVIDPGGVGDGVTVATTRPETMLGDVAVAVHPDDARYAGLVGKHVVLPLTGRTVPVIA
                     DPYVDPQFGTGCVKITPAHDFNDYQVGTRHGLAPLVILTLDARINDNAPAAYRGLDRF
                     EARKRVLADLRAQGLVVSEKPHKLKVPRSNRTGAIVEPMLTDQWFMRMDQLAQRGLDA
                     VRSGAVRFVPEHWTTTYNHWLENIQDWCISRQLWWGHQIPAWYDEAGNVYVARSEDEV
                     RSKFASRLAPQTRLRRDSDVLDTWFSSALVPFTSLGWPEPSRDQELFLPSSVLVTGFD
                     IIFFWVARMIMLTLHFTGKAPFRDVYINAIVRDAEGQKMSKSKGNTLDPLDLIDGI"
BASE COUNT          344 a          707 c          717 g          298 t
ORIGIN      
        1 cagcgcccga ctttcgagcc cgaaattccg gtgctggagg aggcgatcga ggcgctcgac
       61 gaagcggcgc tgcaatgggt caccgccgga gaccggcgcc gccaactgca ggtggcgctg
      121 tacctgcgcc tgcgccagcg cctggatcgg gagatcgagg cgctggcgca aggccgcacg
      181 caggccctcg acgcgaggct cacgcgcgtc gtgcaggccc tgcgtgacgc gctgccggcc
      241 atcgtgcgcg agtcggtcga tcaggcgctg ggcggtcggt cgccaggggt cggcggtcag
      301 tcgtcggtga gagacgacga cgcgaacgac cgctgacagc cggcggccga agcccgacaa
      361 ccgatagaga accgatggaa ctcgccaaga gcttcgagcc ccgggagatc gagcgccgct
      421 ggtaccagat ctgggaatcg cgcggctatt tctcggccag cctcgcatcg gaggcgccgc
      481 cgtattgcat ccagctgccg ccgcccaacg tcaccggcac gctgcacatg gggcacgcct
      541 tccagcagac gctgatggac gcgctgatcc gctaccaccg catgcgcggt ttcaatacca
      601 actgggtcgc gggcaccgac cacgccggca tcgccaccca gatcgtggtc gagcgccagc
      661 tcgaggccga gggcaagagc cgccacgatc tggggcgcga ggctttcgtc gcgcgcgtgt
      721 ggcgatggaa gcagcaatcg ggtgccgcga tcacgcgcca gatgcggcgg ctgggcgcct
      781 cggccaactg gacctacgcc gacaccgagg ggcagcgggc cggctacttc accatggacg
      841 cgcgcatgtc gcgcgcggtg atcgaagtgt tcgtgcgcct gcacgaggag gggctgatct
      901 atcgcgggaa gcgcctggtg aactgggacc cggtgctggg caccgccgtg tccgacctgg
      961 aagtggacaa cgaggaagag gacggccgca tctgggagat ccgctacccg gcgcccggcg
     1021 atggcaccgg cgtgatcgac cccggcgggg tgggagatgg cgtgacggtg gccaccacgc
     1081 gtcccgagac catgctgggc gacgtcgcgg tggcggtgca ccccgacgac gcgcgctacg
     1141 ccggcctggt gggaaagcac gtggtgctgc cgctcaccgg gcgcaccgtt ccggtgatcg
     1201 ccgaccccta cgtcgacccg cagttcggca ccggctgcgt gaagatcacg cccgcgcacg
     1261 acttcaacga ctaccaggtc ggtacgcggc acgggctggc gccgctcgtc atcctgaccc
     1321 tcgatgcgcg catcaacgac aacgccccgg cggcctaccg cggtctggac cgtttcgaag
     1381 cgcgcaagcg ggtgctcgcc gatctgcggg cgcaaggcct ggtcgtgtcg gagaagccgc
     1441 acaagctgaa ggtgccgcgc tcgaaccgca ccggcgcgat cgtcgagccg atgctgaccg
     1501 accagtggtt catgcgcatg gaccaactgg cgcagcgcgg cctggacgcg gtgcgctcgg
     1561 gcgcggtgcg cttcgtgccc gagcactgga ccaccacgta caaccactgg ctcgagaaca
     1621 tccaggactg gtgcatctcg cgccagctct ggtggggcca ccagatcccg gcctggtacg
     1681 acgaggccgg caacgtgtac gtcgcgcgca gcgaagacga agtgcgcagc aagttcgcct
     1741 cgcgtctcgc gcctcagact cgactgcgcc gcgacagcga cgtgctggac acctggttct
     1801 cctcggcgct ggtgccgttc acctcgctcg gctggccgga gccgtcgcgc gatcaggagc
     1861 tgttcctgcc ctcctcggtg ctggtcaccg gcttcgacat catcttcttc tgggtcgccc
     1921 ggatgatcat gctgaccttg cacttcaccg gcaaggcgcc gttccgcgac gtgtacatca
     1981 acgccatcgt gcgcgacgcc gaaggccaga agatgagcaa gtcgaagggc aacacgctgg
     2041 atccgctcga cctgatcgac ggcatc
//
LOCUS       DASPGE010000292         4845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_545138, whole genome shotgun sequence.
ACCESSION   DASPGE010000292 DASPGE010000000
VERSION     DASPGE010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545138"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="VD791_09395"
     CDS             <1..1090
                     /locus_tag="VD791_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXV08232.1"
                     /translation="DEWDAIALNYTSGTTGNPKGVVLHHRGAYLNAVANAVTWNMPAL
                     PVYLWTLPMFHCNGWCFPWTLAMLAGTSVCLRKVEAAAVFDLIRAHRVSHFCGAPVVH
                     NMLVNAPAELRAGIAHKVHGMIAASAPPQALIEGTERMGFDLTHVYGLTEVYGPAAVC
                     AKHPEWSALSLEQRAQMNGRQGVPYELQEDMIVADPQTLRPVPQDGQTVGEIFFRGNI
                     TMKGYLKNPTATEEAFRGGWFHTGDLAVVYPDGYAKIKDRSKDIIISGGENISTLEVE
                     DVLYRHPAVLEAAVVAQPDERWGESPCAFVTLKSGAAPPSAEEMIAFCRERLAHYKCP
                     RKVVFGPLPKTSTGKIQKNVLREQAKAL"
     gene            1124..1879
                     /locus_tag="VD791_09400"
     CDS             1124..1879
                     /locus_tag="VD791_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HXV08233.1"
                     /translation="MKVLVPVKRVVDYNVKVRVKSDGTGVETANVKMSMNPFDEIACE
                     EAVRLKEAGVATEIVAVSIGPTQCQETLRTALAIGADRAILVEAGAEVQPLAVAKILH
                     KIVQNEAPRLVILGKQAIDDDSNQTGQMLAALLGWPQATFASKLKIAGGGAEVTREVD
                     GGLETIAIGLPAVVTTDLRLNEPRYVTLPNIMKAKKKTLDITKPEALGVDVAPRLKTL
                     KVQEPPKRKAGVQVKTVDELVHKLKNEAKVIQP"
     gene            1876..2805
                     /locus_tag="VD791_09405"
     CDS             1876..2805
                     /locus_tag="VD791_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXV08234.1"
                     /translation="MTVLVVAEHDNAGLRAATLNTVTAAQRIGAPVHILVAGSNCGGA
                     AAAAARVPGVEKVRVADAPHYVHPLAESIAALVVALAEGYSHILAAATTTGKNFMPRV
                     AALLDVAQVSDIVAVDGADTFVRPIYAGNALATVQSGDRIKVITVRTTGFDAAPAQGG
                     SAAVEQVSAAADPGQSRLLAQELTKSARPELTAARVIVSGGRGMGSGENFKILEALAD
                     KLGAAVGASRAAVDSGFVPNDYQVGQTGKVVAPELYIAVGISGAIQHLAGMKDSKVIV
                     AINKDGEAPIFQVADYGLVADLFQVVPELTSKL"
     gene            2827..4620
                     /locus_tag="VD791_09410"
     CDS             2827..4620
                     /locus_tag="VD791_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXV08235.1"
                     /translation="MAEYVAPLRDMKFVIKELGALRAVAGLPGCEEVTPDLVDAVLEE
                     AGKFAAGVLSPLNWPGDRAGCKLDNGVVTTPPGFKQAYRQFREAGWPALGGDPEFGGQ
                     GLPHALSGMVSEIWNSANMSFCLCPMLTNGALNAIKRHGSEQQKRTYLEKLVSGAWTG
                     TMNLTEPQAGSDLSAVRTRAVPDGDRYRLHGTKIFITYGEHDFAENIVHLVLARTPDA
                     PDGVKGISLFVVPKYLVNADGSVGARNDVKCVSIEHKLGIHASPTCVMAFGEKEGAIG
                     YLVGEENRGLEYMFTMMNLARLEVGIEGVAIAERAYQRALDYARQRVQGREIGARSGE
                     RVTIIHHPDVRRMLMSMKAQVEAMRALAAAACTALDIAHRHPQREQRLQHQGLFDLLT
                     PVVKGWCTEQSIEVASIGVQVHGGMGFVEETGAAQHLRDARITTIYEGTTGIQANDLV
                     GRKLAAEKGMTAKAVIAEMRAFDTQLAGVSHPALGVIRRALAEGVATLSDATDWLLET
                     YPHNSKAAAAGAVPYLKLWGTVAGGWQMARAALVAKQRLDEGAEDFDFYRGKLGTARF
                     YAEHILPLAHVHRQAIVNGSASVLALEEAQF"
BASE COUNT          836 a         1610 c         1709 g          690 t
ORIGIN      
        1 cgacgaatgg gacgcgatcg cgctcaacta cacctcgggc accaccggca accccaaggg
       61 cgtggtgctg catcaccgcg gcgcgtatct caacgccgtg gccaacgccg tcacgtggaa
      121 catgccggcg ctgccggtgt atctgtggac gctgcccatg ttccactgca acggctggtg
      181 ctttccctgg acgctggcga tgttggcggg aaccagcgtg tgcctgcgca aagtggaagc
      241 cgccgcggtg ttcgatctga tccgcgcgca ccgcgtcagc cacttctgcg gcgcgccggt
      301 ggtgcacaac atgctggtca acgcgccggc ggagctgcgc gcgggcatcg cgcacaaggt
      361 gcacggcatg atcgcggcct ccgctccgcc ccaggcgttg atcgaaggca cggagcgcat
      421 gggcttcgat ctcacgcatg tttacggcct gaccgaggtg tacggaccgg ccgccgtgtg
      481 cgccaagcat cccgaatgga gcgcgctgtc gctggagcag cgcgcgcaga tgaacggcag
      541 gcagggcgtc ccctacgagc tgcaggaaga catgatcgtg gccgatccgc agacgctgcg
      601 gccggtgccg caggacgggc agaccgtggg cgagatcttc ttccgcggca acatcaccat
      661 gaagggatac ctgaagaatc cgaccgccac cgaggaggcc ttccgcggcg gctggttcca
      721 caccggcgac ctcgccgtgg tgtatccgga cggctacgcc aagatcaagg accgttccaa
      781 ggacatcatc atctccggcg gcgagaacat ctcgacgctg gaggtggagg acgtgctgta
      841 ccggcatccc gccgtgctcg aagcggcggt ggtggcgcag cccgacgaga ggtggggcga
      901 gtcgccctgc gccttcgtca cgttgaagag cggcgccgcg ccgccgagcg ccgaggagat
      961 gatcgcgttc tgccgcgagc gcctggcgca ctacaagtgc ccgaggaagg tggtgttcgg
     1021 cccgctgccc aagacctcga ccggcaagat ccagaagaac gtgctgcgcg agcaggcgaa
     1081 ggcgctatag aatcccggct cccgaaaagc acgaggcagg cagatgaaag tcctggttcc
     1141 ggtcaagcgc gtggtggact acaacgtcaa ggtgcgggtg aaatccgacg gcaccggcgt
     1201 ggagaccgcc aacgtcaaga tgtcgatgaa cccgttcgac gagatcgcct gcgaggaagc
     1261 ggtgcggttg aaggaggcgg gcgtggccac cgagatcgtg gccgtttcca tcggccccac
     1321 gcagtgccag gagaccctgc gcaccgcact cgctatcggc gccgaccgcg ccatcctggt
     1381 cgaggcaggc gccgaggtgc agccgctcgc ggtggcgaag atcctgcaca agatcgtgca
     1441 gaacgaggcg ccgcggctgg tgatcctggg caagcaggcg atcgacgacg actcgaatca
     1501 gaccgggcag atgctcgctg cgctgctcgg ctggccgcag gcgacgttcg cttccaagct
     1561 caagatcgcc ggcggcggcg ccgaggtcac gcgcgaggtg gacggcggcc tggagaccat
     1621 cgccatcggc ctgcccgcgg tggtcaccac cgatctgcgc ctgaacgagc cgcgctacgt
     1681 gacgctgccc aacatcatga aggcgaagaa gaagacgctc gacatcacca agcccgaggc
     1741 gctcggcgtg gacgtcgcgc cgcggctcaa gaccctcaag gtgcaggagc cgcccaagcg
     1801 caaggccggc gtgcaggtga agaccgtgga cgagctggtg cacaagctca agaacgaagc
     1861 caaggtgatc cagccatgac ggtcctggtc gtcgccgagc acgacaacgc cggcctgcgg
     1921 gccgcgacgc tcaacaccgt gacggccgcg cagcggatcg gcgctccggt gcacatcctg
     1981 gtcgcgggca gcaactgcgg cggcgccgcg gcggccgccg cccgcgtccc cggcgtggag
     2041 aaggtgcgcg tggccgacgc gccgcactat gtgcatccgc ttgcggagag catcgccgcg
     2101 ctggtggtcg cgctggccga gggctattcg cacatcctgg cggcggccac caccaccggc
     2161 aagaatttca tgccgcgggt cgcagctctg ctcgacgtgg cgcaggtgtc ggacatcgtc
     2221 gcggtggacg gcgccgacac gttcgtgcgt ccgatctacg ccggcaacgc gctcgccacc
     2281 gtgcagtccg gcgacaggat caaggtgatc acggtgcgca ccaccggctt cgacgccgcg
     2341 cccgcgcagg gtggttcggc ggcagtggaa caagtgagcg cagcggccga cccgggtcag
     2401 tcccgattgc tcgcgcagga gctcaccaag tccgcgcgcc ccgagctgac cgcggcccgc
     2461 gtcatcgtct cgggcggacg cggcatgggc agcggcgaga acttcaagat cctggaggcg
     2521 ctcgccgaca agctcggcgc ggcggtcggc gcctcgcgcg cggcggtgga ctcggggttc
     2581 gtgcccaacg actaccaggt gggacagacc ggcaaggtgg tggcgccgga gctctacatc
     2641 gccgtgggca tctccggcgc cattcagcat ctggccggga tgaaggacag caaggtgatc
     2701 gtcgccatca acaaggacgg cgaggcgccg atcttccagg tggccgacta cgggctggtg
     2761 gccgacctgt tccaggtcgt gccggagctg acctccaagc tctgaggcaa cagggaggat
     2821 tcgatcatgg ccgagtacgt cgcacctctg cgggacatga agttcgtgat caaggagctc
     2881 ggcgcgctgc gcgcggtggc gggcctgccg ggctgcgaag aggtcacgcc tgacctggtg
     2941 gacgccgtgc tcgaggaggc cggcaagttc gccgccggtg tgctctcgcc gctcaactgg
     3001 cccggcgatc gcgccggctg caagctcgac aacggcgtgg tcaccacgcc gcccggcttc
     3061 aagcaggcct accggcagtt ccgcgaggcc ggctggccgg cgctgggcgg cgatcccgag
     3121 ttcggcgggc agggcctgcc gcacgcgctg tcgggcatgg tgtcggagat ctggaacagc
     3181 gccaacatga gcttctgcct gtgcccgatg ctcaccaacg gcgcgctcaa cgccatcaag
     3241 cgccacggtt ccgagcagca gaagcgcacc tacctggaga agctcgtctc cggcgcctgg
     3301 accggcacca tgaacctcac cgagccgcag gccggttcgg acctctcggc ggtgcgcacg
     3361 cgcgccgtgc ccgacggcga ccgctaccgc ctgcacggca ccaagatctt catcacctac
     3421 ggcgagcacg acttcgccga gaacatcgtc cacctggtcc tggcgcgcac gcccgatgcc
     3481 cccgatggcg tgaaggggat ctcgctgttc gtggtgccca agtatctggt gaacgcggac
     3541 ggctccgtgg gcgcgcgcaa cgacgtcaag tgcgtgtcga tcgagcacaa gctcggcatc
     3601 cacgcgagcc ccacctgcgt catggccttc ggcgagaagg agggtgcgat cggctatctc
     3661 gtcggcgagg agaaccgcgg gctcgagtac atgttcacca tgatgaacct ggcccggctc
     3721 gaggtcggca tcgaaggcgt ggccatcgcc gagcgcgcct accagcgtgc gctggactac
     3781 gcgcggcagc gcgtccaggg acgcgagatc ggggcgcgat ccggcgagcg cgtcaccatc
     3841 atccatcacc ctgacgtgcg ccgcatgctg atgagcatga aagcgcaggt cgaagcgatg
     3901 cgcgcgctgg ccgcggccgc ctgcaccgcg ctcgacatcg cccatcgcca tccgcagcgc
     3961 gagcaacgct tgcagcacca ggggctgttc gacctgctca ccccggtggt gaagggctgg
     4021 tgcaccgagc agtcgatcga ggtcgcctcg atcggcgtgc aggtgcacgg cggcatgggc
     4081 ttcgtggagg agaccggcgc cgcgcagcat ctgcgcgacg cgcgcatcac cacgatctac
     4141 gaaggcacca ccggcattca ggccaacgac ctggtggggc gcaagctcgc cgccgagaag
     4201 ggcatgacgg cgaaggcggt gatcgccgag atgcgcgcct tcgacacgca actggccggg
     4261 gtcagccatc ccgcgctcgg cgtgatccgg cgcgcgctcg cggagggggt cgcgacgctg
     4321 tcggacgcca ccgactggct gctggaaacg tatccccata acagcaaggc cgcggccgcc
     4381 ggcgcagtgc cctacctcaa gctgtggggc acggtagcgg gcggctggca gatggcgcgc
     4441 gcggcgctgg tggccaagca gcgcctggac gagggggccg aggacttcga tttctaccgc
     4501 ggcaagctcg gcacggcgcg gttctacgcc gagcacatcc tgccgctcgc gcatgtccac
     4561 cggcaggcca tcgtcaacgg gtcggccagc gtattggcgc tggaggaagc gcagttctga
     4621 gggcctccgg ccgtcgccgg aagcagtcgc tgctcagccc ggtccttgct tcgcggtcga
     4681 tacctcgacc cgggcgcccg gcgtcttgac aacgggggcg tcttgacaaa gcgggctgct
     4741 tgacaacgcg tcaggggcga gcgcaaggtg caaggcaccc ggtggcgcgc tgtgttgtgg
     4801 gtgtagagag caggcaatcg agcaggagaa tgcgatggtg atttt
//
LOCUS       DASPGE010000293         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_545208, whole genome shotgun sequence.
ACCESSION   DASPGE010000293 DASPGE010000000
VERSION     DASPGE010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545208"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..505)
                     /locus_tag="VD791_09415"
     CDS             complement(110..505)
                     /locus_tag="VD791_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXV08236.1"
                     /translation="MTRARAGRASSKREASARAPRPQIRIMLRKAIAMGPGKAELLRA
                     IEETGSISAAARTLGMSYRRAWLLVDTMNQCFRSPVVETLTGGQRGGGARVSELGHEV
                     LRRYLEMEAKAAASVQKELAQFTRLMAGH"
     gene            complement(502..963)
                     /gene="moaE"
                     /locus_tag="VD791_09420"
     CDS             complement(502..963)
                     /gene="moaE"
                     /locus_tag="VD791_09420"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HXV08237.1"
                     /translation="MPVRVQAEDFDVGKEIEALRRGNPAVGAIASFIGLVRDVSEGER
                     VAEMTLEHYPGMTEHALEQIVAQAKSRWDIIDVLVLHRIGRLRPLDQIVLVAVTGAHR
                     GEAFSACEFVMDYLKTQAPFWKKEQTPAGARWVEARAADDRAADRWSEPKR"
     gene            complement(967..1269)
                     /gene="moaD"
                     /locus_tag="VD791_09425"
     CDS             complement(967..1269)
                     /gene="moaD"
                     /locus_tag="VD791_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HXV08238.1"
                     /translation="MDNSDLYQGPAVEQQSAVRVLYFARLKEVLGRNAERLALPPGVD
                     TVAALRAHLQARGGVWASELAPGKPVRVAVNQDMATGETRVRAGDEVAFFPPVTGG"
     gene            1470..>2038
                     /locus_tag="VD791_09430"
     CDS             1470..>2038
                     /locus_tag="VD791_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXV08239.1"
                     /translation="MPPRRYTSPLEDTRRRPLRDLRVSVTDRCNFRCTYCMPKEVFGK
                     DFQFLERSQLLTFGEIHRLVRIFRDHGVEKIRITGGEPLVRRKLERLIEMLAYWDDLD
                     LTLTTNGSLLEQKARDLRSAGLRRITVSLDSLDDAVFKAMNDVDFPVRSVLAGIDAAA
                     AAGLHPIKINMVVQRGVNDHSVLEMARFFR"
BASE COUNT          302 a          785 c          640 g          311 t
ORIGIN      
        1 gcacatcctg ctgccggtga tcccggtttc ggcaaattga tcccggcctc gacaaaccga
       61 tccggccctc ggcggactga tcccgccccc gccgccgcac tcctgcgctt caatgccccg
      121 ccatgagccg ggtgaactgc gccagctcct tctgcacgct ggcggcggcc ttggcctcca
      181 tctccaggta gcggcgcagc acctcgtggc ccagctcgct cacgcgcgcc ccgcccccgc
      241 gctgcccgcc ggtgagggtc tcgaccaccg gcgatctgaa gcattggttc atggtgtcca
      301 ccagcagcca ggcgcggcgg tacgacatgc ccagcgtgcg cgccgcggcc gagatggatc
      361 cggtttcctc gatggcgcgc agcagctcgg ccttgcccgg ccccatggcg atggccttgc
      421 gcagcatgat gcggatctgc gggcgcggcg cccgcgccga cgcctcgcgc ttgctggatg
      481 cccttccggc tctcgccctg gtcaacgctt cggctccgac cagcgatcgg cggcccgatc
      541 gtcggccgcg cgcgcttcca cccagcgcgc gcccgccggc gtctgctcct tcttccagaa
      601 cggcgcctgg gtcttgaggt aatccatcac gaactcgcag gcggagaacg cctcaccgcg
      661 gtgagcgccg gtgaccgcca ccagcacgat ctggtccagc ggccgcagcc gtccgatgcg
      721 gtgcagcacc agcacgtcga tgatgtccca gcgcgacttc gcctgcgcca cgatctgctc
      781 cagcgcgtgc tccgtcatcc ccggatagtg ctccagcgtc atctcggcga cgcgctcgcc
      841 ctcgctcacg tcccggacca ggccgatgaa gctggcgatg gcgccgaccg cgggattgcc
      901 ccgccgcagc gcttcgatct ctttgccgac gtcgaaatcc tcggcctgga cccgcaccgg
      961 cacgctctag cccccggtca ccggcgggaa gaaggcgacc tcatcgccgg cgcggacgcg
     1021 cgtctccccg gtcgccatgt cctgattgac ggccacgcgc accggcttgc ccggcgccag
     1081 ctcgctcgcc cagaccccgc cgcgcgcctg caggtgcgcg cgcagggccg cgaccgtgtc
     1141 cacaccgggc ggcaacgcca ggcgctccgc gttgcggccc agcacctcct tcagccgcgc
     1201 gaagtacagc acccgcacgg ccgactgctg ctcgaccgcc ggcccctggt acaggtccga
     1261 gttgtccatc ctggccgaat cccgcgtgag cttcgatgcc ctgcattata atccgctatg
     1321 tccaaacggg atcaacgcct gcccgcagga gcagtcctcg cggatagggc ggcaaccccc
     1381 ccggacggag cagtcacgta gtgggacgcg gccaaatgga caacgcagac gaccataatc
     1441 cacacccgcg ggcgccggag cgcggtccgg tgccgccgcg ccggtacacc tcgccgctcg
     1501 aggacacgcg acgccggccg ctgcgcgatc tgcgcgtctc ggtcaccgac cgctgcaact
     1561 tccgctgcac ctactgcatg cccaaggagg tgttcggcaa ggacttccag ttcctggagc
     1621 gcagccagct gctcaccttc ggcgagatcc accgcctggt gcgcatcttc cgcgatcacg
     1681 gcgtggagaa gatccgcatc accgggggcg agccgctggt gcggcgcaag ctcgagcggc
     1741 tgatcgagat gctcgcctac tgggacgatc tcgatctcac gctcaccacc aacggctcgc
     1801 tgctcgagca gaaggcgcgc gatctgcgca gcgccggcct gaggcgcatc accgtgagcc
     1861 tggactcgct cgacgatgcg gtgttcaagg cgatgaacga cgtggacttc ccggtgcgca
     1921 gcgtgctcgc cgggatcgac gccgcggccg ccgccgggct gcaccctatc aagatcaaca
     1981 tggtggtgca gcgcggcgtc aacgaccaca gcgtcctgga gatggcgcgc ttcttccg
//
LOCUS       DASPGE010000294        13034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_77280, whole genome shotgun sequence.
ACCESSION   DASPGE010000294 DASPGE010000000
VERSION     DASPGE010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13034
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77280"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4083
                     /locus_tag="VD791_09435"
     CDS             <1..4083
                     /locus_tag="VD791_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HXV08240.1"
                     /translation="DNGVGLASAFHSIFEWFLAPGGDPNNAPDVVNNSWAFIAAGCNS
                     EFAPDIEAWRAAEIFPAFAAGNAGPSAGSVRSPGAYAESFAVGATDVFDEVASFSSRG
                     PSPCTALDKPDLAAPGAAIHSALPGNSYGVLSGTSMATPHVAGAVAVLRSIKPDMTVE
                     DLAIALTLGATDIAQPGPDHDSGAGRLDLLTSAEIAINGPSAPVVKVVATTPTVSETG
                     PDGVLTFTRTGDTASPLQINISVAGTASPGSDYIALPLSVTIPEGSASATLAITPIDD
                     AIGEINETVLVSIEPDPAYIRGWSRVASVIMVSDEQLPDLTVTAMSTPASASAGDTVT
                     VSETTNNVGAGPAPATLTQYFLSADVTLDASDVALGSRAVPALGSGTSSTGSAAVTLP
                     SGTANGLWYLIAKADGDDALPESSEFNNTTWRSIQIGVDLVVYGMSGPSNVSPGQSIT
                     VSDTTRNQGAGTAPASVTQFFLSSNGTLDAADIALGSRVVPPLVAGASSSGATALTIP
                     AGTATGYWYVLAKADGPGEVPEASETNNVGSWSIVIGADLIVYSLTGPPTAAAGQSIT
                     ISDTVRNQGSASAPASVTRYFLSADTMLDAGDVALGERSVPALSAGASDTASSALTLP
                     VGTATGAWYVIAKADAGGTVPETSEVNNLNSRWIQIGADLMVSAWSAPSSAGAGESIT
                     VTDTTRNQGGGVAQPSVTQYFLSANSILDGADIALGSRTVPALESGASSTGSITLTLP
                     AATANGTWYLIARVDATQTVAEISEYNNTAQRTMLVGVDLIVSALSAPAAAGAGQSIG
                     VSDTTRNQGGGVAPASLTQFFLSSNNTLDAADIALGSRPVPVLASGASNSGSTTLVIP
                     AGTAAGTWYVIANADGSGVVPETSESNNVGTWAIQIGADLVVSALTVPASAGPGQAIT
                     VSDTTRNQAGGAAAATVTQYFLSGDAVLDAGDVALGSRNVAALAGGASSSASVSVTIP
                     AGTATGNWFVIAKADATDAVLEMVETNNTASRSIQIGPDLSVTSLSAPSAAGAGGTIT
                     VTDTTRNLSTGSAPETLTQYFLSTNTTLDVSDVLLGARAVPALAPGASSSGSVSVTLP
                     SGTATGVWYLIAKADGNDSLPESSETNNVTWRSLQIGVDLVVSPLSGPAGANAGQVVS
                     VGDTTRNQGGGAALASTTQFFLSSNSALDAADLPLGSRAVPALAAGASSSGATTLTIP
                     AGTATGYWYILAKADAPGDVLEISEGNNVTAWLMTVGPDLIAYSLIVPGTAPAGQTIT
                     VTDTTRNQGGGDAAASTTQFFLSRDMAWDAADIALGGRTVGTLAGGAASTASTALTIP
                     AETPTGGWYLLGRVDAGNAVVEIAEFNNSSARWIQITAPQ"
     gene            4198..5973
                     /locus_tag="VD791_09440"
     CDS             4198..5973
                     /locus_tag="VD791_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HXV08241.1"
                     /translation="MMRAFDRLAVLAICSSFAAAAHALRPGDRVDNFMLLDQHGASHE
                     LYYLSDAKAVVLMVHGCGCPIVRNTLPGLEEIRDRYRAQGVEFLLINSNLQDDRAAVA
                     KETAEFTIDFPILLDRTQLVGESLGVARTADVFVIDPRSWTLVYRGPIDDRLAYGKQK
                     PVATHAYLSAALDALLAGEPVTVAQAETAGCLVHLPERDRREQHAQISYSERIAPLLM
                     QRCASCHRASGVAPWAMTGYATVRGFAPMMREVVRTGRMPPWHADPHYGSFVGDRSMT
                     EEEIRTLVHWIEAGAPRGEGPDPLAELESARSEWTLGEPDLVVEVPAFEVPATGVVDY
                     QYPRLPNPMDRDVWVRAVEIIPSERSVVHHVLAGIDDPARSVHRAGQIGELGGYAPGK
                     NPVPYPQDTGVLLRKEASLRFQMHYTPSGKATTDRTRVGYYFHREPPRFALQTTFLMT
                     PSLLIPAHAKAHTESVEHLLERDIVLYSLMPHAHLRGRAARFTAYYPDGGEEILLSVP
                     KYDFGWQTTYALRSPKRLPAGTRIVFDMTWDNSAQNPANPDPARDVPWGDQTWDEMNA
                     GWIRYRDADADDTAAGRSEDLGQAR"
     gene            complement(5984..6649)
                     /locus_tag="VD791_09445"
     CDS             complement(5984..6649)
                     /locus_tag="VD791_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HXV08242.1"
                     /translation="MDFETVMRSRFSARKFFDRQVPERTLQRILELAQHTASWCNCQP
                     WQLVITRGVATERFRLAVHAHARSGARPDPDFPFPAAYEGVYRERRKVCGVQLYQALG
                     IGKEDRGAAAQQALENFRLFGAPHVAIVTTEERLGVYGAIDCGLYVGNFLLAARNEGV
                     DTIAQAALASYPDFVREFFSLPANRKVVCGISFGYADSRDPIHSYRTERAPVAEAVVF
                     VET"
     gene            complement(6698..7105)
                     /locus_tag="VD791_09450"
     CDS             complement(6698..7105)
                     /locus_tag="VD791_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXV08243.1"
                     /translation="MALVKLQDDDGAKPEARAVFDDIRKTRNTDYVNNIWRALAVHPP
                     MLKRFWSYMKEVMVKDSRLDPLTKEYIYLAVSITNNCEYCIHSHIAAARKKGMDDETL
                     AEVVEIVALANAGNRLTTGLQVEVDERFKPQFE"
     gene            7217..7843
                     /locus_tag="VD791_09455"
     CDS             7217..7843
                     /locus_tag="VD791_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HXV08244.1"
                     /translation="MKLLFDLFPVLLFFVAYSLGKRAPDTAAAAVGGVLGAVGLGSQV
                     GVDQAPILLATAVAILATFGQVGWLLARGRQVDKMLWISLGIIVVMGGATLWLRDPTF
                     IKWKPTVLYWAFAAVLLGAEFLFARNLIRAMMERQIALPDAVWARLNLSWGGFFILMG
                     ALNLFVAYNFSESAWVNFKLFGGIGLMLVFVLAQGLFLARFVQNEQGE"
     gene            7849..8229
                     /locus_tag="VD791_09460"
     CDS             7849..8229
                     /locus_tag="VD791_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HXV08245.1"
                     /translation="MAKDAEVAAREEDVASSNIIAAMRERLAALAPDLVEIFDESAAH
                     AGHEGAKGGGGHYRLLIVARAFESASRVARHRMVYAALGALLRREIHALAISAVTPEE
                     LAPREEPGPPETRSPAGRSNRTIR"
     gene            8256..9065
                     /locus_tag="VD791_09465"
     CDS             8256..9065
                     /locus_tag="VD791_09465"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXV08246.1"
                     /translation="MKAASAATLTLAGMLALSACNAQDSSANIAVVNGVPIPHARLDY
                     VMKAQVQQGQKDDEETRKQIKEALITREILAQEAVKKGLDKDPHLQTQVEMAKQEFLI
                     RAYFDDFVKTNGVTDEEMHAEYERVKAQQTDNGERKEYRARHILIKNEKPAKAALAQI
                     NQAKGKNFAQVAKTKSEDTGSKADGGLLDWSDGSNFVKEFSEAMVKLDKGQYTRQLVK
                     TQYGYHIIRLEDVREMQFPPFEQVKERIEQQLLAQKRDKTIEALRTAAKVE"
     gene            complement(9104..12997)
                     /gene="purL"
                     /locus_tag="VD791_09470"
                     /gene_synonym="purI"
     CDS             complement(9104..12997)
                     /gene="purL"
                     /locus_tag="VD791_09470"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HXV08247.1"
                     /translation="MSSVLRLPGRPALSDFRLDKLLHSARKAIPALHGLRTQYWHFVK
                     LKRPLDGHGREVLDALLTYGPRAGPEDLRDDILLVVPRLGTLSPWSSKATDIAHQCGL
                     DAVERIERGTAYRMQLAPGARLDAAGRRALLPLLHDRMTETVLAALDRADALFHEATP
                     RPLAEVDVLHRGAHALADANRSMGLALSDDEIAYLGDYFTRIRRNPTDVELMMFAQAN
                     SEHCRHKIFNADWVIDGVAQPESLFRMIKHTHAQHPAGTLVAYADNAAVMEGARVVRF
                     YPRADGVYRAAEEDVHILMKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGAKPKA
                     GLTGFSVSDLHIPGFEQPWERGYGKPGRIASALQIMLEGPIGAAGFNNEFGRPNLLGY
                     FRTFEQPVAGEQRGYHKPIMIAGGLGSIAAEQVRKQPLAEGAALVQLGGPGLLIGLGG
                     GSASSMGTGDNVEDLDYDSVQRGNAELQRRAQEVIDRCWALGADNPILSIHDVGAGGL
                     SNALPELVHGGGAGGRFDLRAIPSEEPGMSPMQVWCNEAQERYALAIRSDRLDAFRAL
                     CERERCPFAVVGNVTASQQLVVEDPLFGNRPVDMDLSVLLGKPPRMTRHVKRRRHEPP
                     ALDLAGVALEEAALRVLRLPAVADKTFLISIGDRTVGGLCSRDQMVGPWQVPVSDVAV
                     TLMGFDTFRGEAMAMGERTPLALIDAPASGRMAVGEALTNIAAARIRTLGDVKLSANW
                     MAAAGHGGEDAALYDTVKAVGLDLCPALGISIPVGKDSMSMKTAWPEDGRTREVVAPV
                     SLIVSAFAPCVDARATLTPQLRAQHGDTVLLLIDLGAGRNRLGASALAQVYGQLGNSA
                     PDVDDPAALARFFGVVQALNREGRALAYHDRSDGGLFVTLCEMMFASRVGVTVEIDAL
                     LNGGSALAALFNEELGAVLQVRRRDLIAVEEAFAAAGLAAQCHPLGGLNREGRLVVRA
                     HNRILYAESGVSLQRAWSETTYRMQKLRDNPQCALEEYDRLLDTADPGLHAGLTYDPA
                     HDVAAPFIATGERPRIAILREQGVNGQIEMAAAFDRAGFAAVDVHMTDIIGGRVGLKE
                     FKGIVACGGFSYGDVLGAGEGWAKSILFNARARDEFEAFFGRADAFALGVCNGCQMMS
                     NVRALIPGAEHWPHFVRNRSEQFEARFAMVEVTAGPSLFFAGMVGSRMPIAVAHGEGY
                     AEFKSEQQLAAARKLVALRYVDNHGRPGPRYPLNPSGSPEGIAGLTTADGRFTILMPH
                     PERVFRAAQNSWHPEEWGEDGAWMRMFRNARAWLG"
BASE COUNT         2082 a         4595 c         4424 g         1933 t
ORIGIN      
        1 gacaacggcg tcggcctggc ctccgctttc cacagcatct tcgagtggtt cctcgccccc
       61 ggcggcgatc cgaacaatgc gcccgacgtg gtgaacaact cctgggcctt catcgccgcc
      121 ggctgcaaca gcgagttcgc acccgacatc gaggcgtggc gcgccgcgga gatctttccg
      181 gcgttcgccg ccggcaacgc cggaccgagc gccggcagcg tgcgcagccc cggcgcgtac
      241 gccgagtcgt tcgcggtggg ggcgaccgac gtcttcgacg aggtggcgag cttcagcagc
      301 cgcggcccgt cgccctgcac cgcgctcgac aagcccgacc tcgccgcgcc cggtgccgcg
      361 atccattccg ccctgccggg caactcgtac ggcgtgctga gcggcacctc gatggccacc
      421 ccgcacgtgg ccggcgccgt ggcggtgctg cgctcgatca agccggacat gaccgtcgag
      481 gatctggcca tcgcgctcac gctgggcgcc accgacatcg cgcagcccgg ccccgaccac
      541 gactcgggcg cggggcggct ggacctcctg acgtcggcgg aaatcgccat caacggtccg
      601 tcggcgcccg tggtgaaggt ggtcgcgacc acgcccacgg tttccgagac ggggcccgac
      661 ggggtgctca ccttcacccg taccggcgac accgcgagcc ccctgcagat caatatcagc
      721 gtggcgggca cggcgagccc cggcagcgat tacatcgctc tgccgctcag cgtgacgata
      781 ccggagggct ccgccagcgc tacgctcgcg atcacgccga tcgacgacgc gatcggggaa
      841 atcaacgaaa cggtgctggt gagcatcgag cccgatcccg cgtacatccg cggctggtcg
      901 cgcgtggcga gcgtgatcat ggtcagcgac gagcagttgc ccgacctgac cgtgaccgcc
      961 atgtcgacgc cggccagcgc cagcgccggc gacaccgtca ccgtgagcga aaccacgaac
     1021 aacgtcggcg ccgggccggc accggcgacg ctgacgcagt acttcctttc ggccgacgtc
     1081 acgctggatg cgtccgatgt agcgctgggc tcgcgcgcgg tgcccgcgct cgggtcggga
     1141 acgagcagca cggggtcggc tgcggtcacc ttgccgtcgg gcactgccaa cgggctctgg
     1201 tacctcatcg ccaaggccga cggcgacgac gccctgcccg agtcctccga attcaacaac
     1261 accacctggc gctcgattca gatcggcgtg gacctcgtgg tgtacggtat gtcgggacct
     1321 tccaacgtca gccccggcca gagcatcacc gtcagcgaca ccacgcgcaa ccagggcgct
     1381 ggcaccgccc cggcttcggt cacgcagttc ttcctgtcca gcaacggtac gttggatgcc
     1441 gccgacatcg cgctgggcag ccgcgtggtg ccgcccctgg tcgccggcgc gagcagcagc
     1501 ggcgccacgg cgctgaccat cccggcgggc acggccaccg gctactggta cgtcctcgcc
     1561 aaggccgacg ggccaggcga ggtgcccgaa gcctccgaga ccaacaacgt cggcagctgg
     1621 tcgatcgtga tcggcgccga cttgatcgtg tactcgctga ccgggccgcc gaccgccgcc
     1681 gccggccaga gcatcaccat cagcgacacc gtccgcaacc agggcagcgc atccgcgccg
     1741 gccagcgtta cgcgttactt cttgtccgcg gacacgatgc tggatgcggg cgacgtggcg
     1801 ctcggcgagc gcagcgtgcc ggcgctctcc gcgggcgcca gcgacaccgc gtcgagcgcg
     1861 ctcacgctcc ccgtcggcac cgccaccggc gcctggtatg tgatcgccaa ggccgacgcc
     1921 ggcgggaccg tgcccgagac ctccgaggtg aacaacctca actcgcgctg gattcagatc
     1981 ggcgccgacc tgatggtgtc ggcctggtcg gcgccttcca gcgccggcgc cggcgagagc
     2041 atcacggtca cggacacgac gcgcaaccag ggcggcggcg ttgcgcaacc gtccgtcacg
     2101 cagtacttcc tgtcggccaa cagcatattg gacggcgccg acatcgcgct cggcagccgt
     2161 acggtgccgg ccctggagag cggagccagc agcaccggca gcatcacgct cacgctgcct
     2221 gcggcgaccg ccaacggcac ctggtacctg atcgccagag tcgacgcgac gcagactgtg
     2281 gccgagatat cggagtacaa caacaccgcc cagcgcacca tgctggtcgg cgtggacctg
     2341 atcgtgtcgg cgctgagcgc gccggcggca gccggggcgg gccagagcat cggcgtcagc
     2401 gacaccacgc gcaaccaggg cggcggcgtg gcgccggctt cgctcacgca gttcttcctg
     2461 tcgagcaaca acacgctgga tgccgccgac atcgcgctgg gcagccgtcc ggtgccggtg
     2521 ctcgcttccg gggcgagcaa cagcggctcc acgacgctcg tcattccggc ggggacggcg
     2581 gcggggactt ggtacgtgat cgccaacgcc gacggctccg gggtggtgcc ggaaacctcg
     2641 gagagcaaca acgtcggtac ctgggcgatc cagatcggcg ccgacctggt ggtgtccgct
     2701 ctcaccgtgc cggccagcgc cggaccgggg caggcgatca ccgtcagcga caccacccgc
     2761 aaccaggcgg gcggagcggc agcggccacg gtgacgcagt acttcctctc cggcgacgcc
     2821 gtcctcgacg caggcgacgt cgcgctgggc agccgcaacg tggccgccct ggccggcggt
     2881 gcgagcagtt cggcgtccgt cagcgtgacc attcccgcgg gtaccgccac cggcaactgg
     2941 ttcgtcatcg ccaaggccga tgcgaccgat gcggtgctcg agatggtgga aaccaacaac
     3001 accgcttcgc gttcgatcca gatcggcccc gacctgagcg tgacctcgct ctcggcgccg
     3061 agcgcggcgg gcgcgggcgg tacgatcacc gtcaccgaca ccacccggaa cctcagcacc
     3121 ggatcggcgc ccgagacatt gacccagtac ttcctgtcga ccaacactac gctggatgta
     3181 tccgacgtgc tgctgggcgc gcgcgcggtg ccggccctgg cgcccggggc gagcagttcg
     3241 ggctcggtgt cggtgaccct gccgtcgggc acggccaccg gcgtctggta cctgatcgcc
     3301 aaggccgacg gcaacgacag cctgccggag tcctccgaga ccaacaacgt gacctggcgg
     3361 tcgctccaga tcggcgtcga cctggtcgtg tccccgctct cggggccggc cggggcgaac
     3421 gccggccagg tcgtcagcgt cggcgacacc acccgcaacc agggcggggg cgcggcgctg
     3481 gcttcgacca cgcagttctt cctgtcgagc aacagcgcac tggatgccgc cgatctcccg
     3541 ctgggcagcc gcgcggtgcc ggccctggcc gccggcgcaa gcagcagcgg cgcgaccacg
     3601 ctgaccattc ccgcgggcac ggccaccggt tactggtaca ttcttgccaa ggccgacgcg
     3661 cccggggacg tgctcgagat ctcggaaggc aacaacgtca ccgcctggct gatgacggtc
     3721 ggtcccgacc tgattgcgta ttcgctcatc gttcccggca ccgcccccgc cggccagacc
     3781 atcactgtga ccgacaccac ccgcaaccag ggcggcggcg atgccgcggc atcgaccacg
     3841 cagttcttcc tgtcgcgaga catggcgtgg gatgcggccg acatagcgct cggcggccgc
     3901 actgtgggca cgctggccgg aggggccgcc agcaccgcgt ccaccgcgtt gaccatcccg
     3961 gcggaaacgc ccaccggcgg ttggtatctc ctcggcagag tcgacgccgg caacgcggtg
     4021 gtcgagattg cggagttcaa caacagctcc gcgcgctgga ttcagatcac ggcgccgcag
     4081 taggcctgcg gcgccgccta gcgccactct cggaaccggc gggcgcagcg cgccctgagc
     4141 ggacctgccc gggcgcataa tgcgagcaag atccggacat cgctgaggag agcaccgatg
     4201 atgcgcgcgt ttgaccgtct ggcggtgctg gcgatctgct cgtcgttcgc ggccgccgcc
     4261 catgcattgc gacccggcga tcgggtggac aacttcatgc tcctcgatca gcacggagcc
     4321 tcgcacgagc tgtattacct gtcggacgcc aaggccgtcg tgctcatggt gcacggctgc
     4381 ggttgcccga tcgtgcgcaa cacccttccc ggtttggagg agattcgcga ccggtatcgc
     4441 gcccagggcg tggagttcct gctgatcaac tcgaacctgc aggacgaccg agccgccgtc
     4501 gcgaaggaaa cggccgagtt caccatcgac tttccgatcc tgctggatcg cacacagctc
     4561 gtcggcgaat cgctgggggt ggcgcgcacc gccgacgtgt tcgtgatcga cccgcgcagc
     4621 tggacgctcg tgtaccgggg gccgatcgac gaccggctgg cctatgggaa acagaagccg
     4681 gtggcaacgc atgcctatct cagcgccgcg ctggatgcgc tgctggccgg cgagccggtg
     4741 acggtggcgc aggcggagac cgccggctgc ctggtgcacc tgcccgagcg ggaccggcgc
     4801 gagcaacacg cgcagatttc ctattcggag cgcatcgcac cgttgctgat gcagcgctgc
     4861 gcgagctgcc accgcgcgag cggcgtcgcg ccttgggcga tgaccggcta cgcgaccgtg
     4921 cgcggtttcg cgccgatgat gcgcgaagtg gtgcgcaccg gtcgtatgcc gccctggcac
     4981 gccgatccgc attacggcag cttcgtgggc gaccgctcga tgaccgagga agagatccgc
     5041 accctggtgc actggatcga ggccggcgcc ccccgcgggg aggggcccga tccgcttgcc
     5101 gagctggaga gcgcccgctc ggagtggacc ctgggcgagc ccgatctggt tgtggaagta
     5161 cccgctttcg aggtccccgc caccggcgtg gtcgattatc agtatccgcg cctgcccaac
     5221 cccatggacc gcgacgtgtg ggtgcgcgcg gtcgagatca tcccgagcga gcgcagcgtc
     5281 gtgcaccacg ttcttgccgg aatcgacgac ccggcgcgca gcgttcaccg cgccggccag
     5341 atcggggagc tgggcggcta cgcgccgggc aagaacccgg ttccgtaccc gcaagacacc
     5401 ggcgtcctgc tgcgcaagga ggccagcctc cgcttccaga tgcattacac gccgagcggc
     5461 aaggcgacga ccgatcgcac ccgcgtgggc tattacttcc accgcgagcc gccgaggttc
     5521 gcgctgcaga ctacgttcct gatgacgccg tcgctgctga tcccggcgca cgccaaggca
     5581 cataccgagt cggtcgaaca cctgctggag cgtgacatcg tgctctacag cctgatgccg
     5641 cacgcccacc tgcgcggccg cgccgcccgc ttcaccgcct attatccgga cggcggcgag
     5701 gagatcctgc tgtcggtgcc gaagtacgat ttcggctggc agaccactta cgcgctgcgc
     5761 tcgcccaagc ggctgccggc gggcacccgt atcgtgttcg acatgacctg ggacaactcg
     5821 gcgcagaacc cggccaaccc cgatccagca cgggacgtgc cgtggggaga tcagacctgg
     5881 gacgagatga atgcgggctg gatccgctat cgcgatgcgg atgccgacga caccgcggcc
     5941 ggtcgttccg aggacctggg tcaggcgcga tgatgccgcc cggtcaggtc tcgacgaaca
     6001 cgaccgcctc cgccaccggg gcacgctcgg tgcggtagga gtggatcgga tcgcgcgaat
     6061 ccgcgtaacc gaacgagatc ccgcacacca ccttgcggtt cgccggcagg gaaaagaact
     6121 cgcggacgaa gtccgggtag gaggccagcg ccgcctgcgc gatcgtgtcc acgccttcgt
     6181 tgcgcgcagc gagcaggaag ttgcccacgt acaacccgca gtcgatggcg ccgtagacgc
     6241 cgagcctctc ctcggtggtc acgatcgcca catgcggcgc gccgaacagg cgaaagttct
     6301 cgagcgcctg ctgcgccgcc gccccgcggt cctccttgcc gatgcccagg gcctgataga
     6361 gctgcacacc gcacaccttg cgccgctcgc ggtacacgcc ttcgtaggcg gccgggaacg
     6421 ggaagtcggg gtcgggccgc gcgcccgagc gcgcgtgcgc gtgcaccgcg aggcggaaac
     6481 gctcggtggc gacgccgcgg gtgatgacga gctgccaagg ctggcagttg caccaggacg
     6541 cggtgtgctg cgcgagctcg aggatgcgtt ggagcgtgcg ctcgggcacc tgccggtcga
     6601 agaacttccg cgcgctgaag cgcgaacgca taacggtctc gaaatccatg tgtacgccct
     6661 tcggtcacgc ttcccggtca cgcctttcgg tcacgcctca ttcgaactgc ggcttgaagc
     6721 gctcgtccac ttcgacttgc aggccggtgg tcaggcggtt gccggcgttg gcgagcgcca
     6781 cgatctcgac cacctccgcc agggtttcgt cgtccatgcc cttcttgcgc gccgcggcga
     6841 tgtgcgagtg gatgcaatat tcgcagttgt tggtgatgct gaccgcgagg tagatgtact
     6901 ccttggtgag gggatccagg cggctgtcct tgaccatgac ctccttcatg tagctccaga
     6961 agcgcttgag catcggcgga tgcacggcca acgcccgcca gatgttgttc acgtagtcgg
     7021 tgttccgcgt cttgcggatg tcgtcgaaca ccgcccgcgc ttcgggcttc gcgccgtcat
     7081 cgtcctgcag cttgaccagg gccatggttt cctcctgcgg aaaagggctc cgcatcttac
     7141 catccaccct cgcacgggcg gttgcgcggc aggccgcgca agcctcataa tcctacgttt
     7201 cgtcaagccc ttgcccatga agctgctgtt cgacctgttc cccgtcctgc tgttcttcgt
     7261 cgcctacagc ctcggaaaac gcgccccgga tacggccgcc gccgcggtcg gcggcgtgct
     7321 gggcgccgtc gggctcggca gccaggtcgg cgtggaccag gcgccgatcc tgctggccac
     7381 cgcggtggcg atcctggcca ccttcggaca ggtcggctgg ctgctcgcac gcggccgcca
     7441 ggtggacaag atgctgtgga tcagcctggg catcatcgtg gtgatgggtg gcgccacgct
     7501 gtggctgcgc gacccgacct tcatcaagtg gaagcccacg gtcttgtact gggcgttcgc
     7561 ggccgtgctg ctcggcgccg agttcctgtt cgcgcgcaac ctgatccgcg cgatgatgga
     7621 gcggcagatc gcgttgccgg acgccgtctg ggcgcgcctg aacctgagct ggggcggatt
     7681 cttcatcctg atgggcgcgc tcaacctgtt cgtcgcctac aacttctctg agagcgcctg
     7741 ggtcaacttc aagctgttcg gcggtatcgg tttgatgctg gtgttcgtgc tggcgcaggg
     7801 cctgttcctg gcgcgcttcg tgcagaacga gcaaggcgag tagcagatat ggcaaaggac
     7861 gccgaagtgg ccgcgcggga ggaagacgtc gcttcatcga acatcatcgc cgcgatgcgc
     7921 gagcgcctcg ccgcgctcgc tcccgacctg gtggagatct tcgacgagag cgccgcccac
     7981 gccggccacg agggcgccaa gggcgggggc ggccactatc ggctgctgat cgtcgcgcgc
     8041 gccttcgaaa gcgcctcgcg cgtggcgcgc caccgcatgg tctacgcggc cctgggcgcg
     8101 ctgctgcggc gagagatcca cgcgctggcg atcagcgccg tcacgccgga ggagctggcc
     8161 ccgcgggagg agccgggtcc gccggagacc cggtcgcctg ccgggcgatc caaccgtacg
     8221 attcgttaac ccatgaagga atgcacatgc agctcatgaa agccgcatcc gcggcgacgc
     8281 tcaccctggc aggaatgctc gcgctcagcg cctgcaacgc ccaagacagc agcgccaaca
     8341 tcgccgtggt caacggcgtg ccgatcccgc atgcgcgcct ggactacgtg atgaaagccc
     8401 aggtacagca gggccagaag gacgacgagg agacgcgcaa gcagatcaag gaggccctga
     8461 tcacgcgcga gatcctggcg caggaggcgg tgaagaaggg tctggacaag gacccgcacc
     8521 tgcagaccca ggtcgagatg gccaagcagg agttcctgat ccgcgcctat ttcgacgatt
     8581 tcgtcaagac caacggcgtc accgatgagg agatgcacgc cgagtacgag cgcgtgaaag
     8641 cgcagcagac cgacaacggc gaacgcaagg agtaccgggc gcgccacatc ttgatcaaga
     8701 acgagaagcc ggcgaaggcc gcgctcgcgc agatcaatca ggccaagggc aagaacttcg
     8761 cccaggtggc gaagaccaag tccgaggaca ccggctcgaa ggccgacggc ggactgctcg
     8821 actggagcga cggcagcaac ttcgtcaagg agttcagcga ggcgatggtc aagctcgaca
     8881 agggccagta caccaggcag ctggtcaaga cccagtacgg ctaccacatc atcaggctgg
     8941 aggacgtgcg cgagatgcag ttcccgccgt tcgagcaggt gaaggagcgc atcgagcagc
     9001 agctgctggc gcagaagcgc gacaagacca tcgaggcgct gcgcacggcc gccaaggtcg
     9061 agtagcgcgg ccccgggccg gctgctccct ccccggatgc caactacccg agccaggcgc
     9121 gcgcgttgcg gaacatccgc atccaggcgc cgtcttcgcc ccactcctcg gggtgccagg
     9181 agttctgcgc ggcgcggaac acgcgttccg ggtgcggcat caggatggtg aagcggccgt
     9241 ccgcggtggt gagaccggcg atgccttcgg gcgagccgct ggggttgagc ggatagcgcg
     9301 gccccgggcg gccgtggttg tcgacgtagc gcagggccac cagcttgcgc gcggcggcga
     9361 gctgctgctc gctcttgaac tcggcatagc cctcgccatg ggccaccgcg atgggcatgc
     9421 ggctgccgac catgccggcg aagaacagcg aagggccggc ggtcacttcg accatcgcga
     9481 agcgcgcctc gaactgctcc gagcggttgc gcacgaagtg cggccaatgc tccgcgcccg
     9541 ggatcagcgc gcgcacgttg ctcatcatct gacagccgtt gcacaccccg agagcgaacg
     9601 catcggcgcg gccgaagaac gcctcgaact cgtcgcgcgc ccgcgcgttg aacaggatcg
     9661 acttggccca gccctcgcct gcccccagca cgtcgccgta actgaacccg ccgcatgcga
     9721 cgatgccctt gaactccttc aagcccacgc gcccgccgat gatgtcggtc atgtgcacgt
     9781 ccaccgcggc gaaaccggcg cggtcgaagg ccgccgccat ctcgatctgg ccgttgacgc
     9841 cctgctcgcg caggatggcg atccgcggcc gctccccggt cgcgatgaac ggggcggcga
     9901 cgtcgtgcgc cgggtcgtag gtcagcccgg cgtgaagccc cgggtcggcg gtatcgagca
     9961 gccggtcgta ctcctccagc gcgcactgcg ggttgtcgcg cagcttctgc atgcggtacg
    10021 tcgtctccga ccaggcgcgc tgcaggctga cccccgactc ggcgtacagg atcctgttgt
    10081 gcgcgcgcac caccaggcgc ccttcacggt tgaggccgcc cagcggatgg cactgggcgg
    10141 cgagcccggc ggccgcgaac gcctcctcga cggcgatcag gtcgcgccgg cgcacctgca
    10201 gcacggcgcc cagctcttcg ttgaacagcg cggcgagcgc gctgccgccg ttgagcaggg
    10261 catcgatctc caccgtcacg ccgacccggc tcgcgaacat catctcgcac agggtgacga
    10321 acaggccgcc gtcggatcgg tcgtggtagg cgagcgcgcg tccctcccga ttgagcgcct
    10381 gcaccacgcc gaagaatcgc gcgagcgctg ccgggtcgtc cacatccggc gccgagttgc
    10441 cgagctgacc gtacacctgc gccagcgccg aggccccgag gcggttgcgg ccagcgccca
    10501 ggtcgatcag cagcagcacc gtgtcgccgt gctgggcgcg cagctgcggc gtgagcgttg
    10561 cgcgggcatc cacgcacggc gcgaacgcgg acacgatcag ggacaccggc gccaccacct
    10621 cgcgcgtgcg gccgtcttcc ggccaggccg tcttcatcga catggagtcc ttgcccacgg
    10681 ggatgctgat ccccagcgcg ggacacagat ccagtcccac cgccttgacc gtgtcgtaga
    10741 gcgcagcgtc ttcgccgccg tgcccggccg ccgccatcca gttggcagac agcttcacgt
    10801 ctccgagcgt tcggatgcga gcggcggcga tgttggtgag cgcctcgccc acggccatgc
    10861 ggccagaggc cggcgcgtcg atcagcgcca gcggagtgcg ctcgcccatg gccatcgcct
    10921 cgccgcggaa ggtgtcgaat cccatcagcg tgaccgccac gtcggatacc ggcacttgcc
    10981 agggccccac catctggtcg cggctgcata acccgcccac cgtgcggtcg ccgatgctga
    11041 tcaggaaggt cttgtcggcg accgccggca ggcgcagcac gcgcagcgcc gcctcttcca
    11101 gcgcgacgcc ggcgaggtcg agggccggcg gctcatgacg cctgcgcttg acgtgccgcg
    11161 tcatcctggg cggcttgccc agcagcaccg acagatccat gtccaccggt cggttgccga
    11221 acagcggatc ctcgaccacg agctgttgcg acgccgtcac gttgccgact accgcgaaag
    11281 ggcagcgttc gcgctcgcac aatgcgcgga aagcatccag ccggtcgctg cggatcgcga
    11341 gcgcatagcg ctcctgcgcc tcgttgcacc acacctgcat cggcgacatg cccggctcct
    11401 cgctcgggat cgcgcgcagg tcgaaacgcc cgcccgcccc gccgccgtgc accagctcgg
    11461 gcagcgcgtt ggacaacccg cctgccccga cgtcgtggat cgacaggatc gggttgtccg
    11521 cgcccagcgc ccagcagcgg tcgatgactt cttgcgcgcg ccgctgcagc tctgcgttgc
    11581 cgcgctggac cgagtcgtag tccaggtcct cgacgttgtc gccggtgccc atgctcgagg
    11641 ccgaaccccc gcccagcccg atcagcaagc cgggaccgcc gagctgcacc agcgcggcgc
    11701 cctccgccag cggctgcttg cggacctgct cggccgcgat gctgcccagc ccgccggcga
    11761 tcatgatcgg cttgtggtag ccgcgctgct cgccggcgac gggctgctcg aaggtacgga
    11821 agtagccgag tagattgggg cggccgaact cgttgttgaa gccggccgcg ccgatcgggc
    11881 cctcgagcat gatctgcagc gcggaggcga tgcgccccgg cttgccgtag ccccgctccc
    11941 acggctgctc gaagccggga atgtgcagat cggacacgct gaagccggtg aggccggcct
    12001 tgggtttcgc gccgcgtccg gtcgcgccct cgtcgcggat ctcaccgccg gagccggtgg
    12061 ccgcgcccgg gaacggcgag atggcggtgg gatggttgtg cgtctccacc ttcatcagga
    12121 tgtgcacatc ctcctcggct gcgcgataca cgccgtccgc gcgcgggtag aaacgcacga
    12181 ctctcgcccc ctccatgacg gcggcgttgt ccgcgtaggc caccagcgtg ccggccggat
    12241 gctgcgcgtg ggtgtgcttg atcatgcgga acagagactc gggctgcgcg accccgtcga
    12301 tcacccagtc cgcgttgaag atcttgtgcc ggcaatgctc ggagttcgcc tgggcgaaca
    12361 tcatcagctc cacgtccgtg gggttgcgcc ggatgcgggt gaagtagtcg ccgaggtagg
    12421 cgatctcgtc gtcggacagc gccaggccca tgctgcggtt ggcgtcggcc agagcgtgcg
    12481 cgccccggtg cagcacatcg acctcggcca gcggccgcgg cgtggcttcg tggaacagcg
    12541 catcggcccg gtccagagcg gccagcacgg tctctgtcat gcgatcgtgc agcagcggca
    12601 acagcgcgcg ccggcccgcg gcgtcgagcc gcgcgcccgg cgcgagctgc atgcgatacg
    12661 ccgtgccgcg ctcgatgcgc tccacggcat ccaggccgca ttggtgcgcg atgtcggtcg
    12721 cctttgagga ccagggcgaa agcgtgccca gacgcggcac caccagcagg atgtcgtccc
    12781 gcaagtcctc cggcccggct ctcgggccat acgtgagcag cgcgtccagc acctcgcggc
    12841 cgtggccgtc gaggggccgt ttgagcttca cgaagtgcca gtactgggtg cgcaggccgt
    12901 gcaaggcggg gatcgctttg cgggccgagt gcaagagctt gtcgagacgg aaatcggaga
    12961 gcgcgggacg gccgggaagc cggaggacag aggacatcgc aggtcgagtg aacccgtaag
    13021 atgcgattat actc
//
LOCUS       DASPGE010000295        12698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_663007, whole genome shotgun sequence.
ACCESSION   DASPGE010000295 DASPGE010000000
VERSION     DASPGE010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12698
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663007"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1926
                     /locus_tag="VD791_09475"
     CDS             <1..1926
                     /locus_tag="VD791_09475"
                     /EC_number="1.20.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050611 - arsenate reductase (azurin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (azurin) large subunit"
                     /protein_id="HXV08248.1"
                     /translation="AIRTPLVRIHNRPAYNSECHATREMGIGELNNSYEDAELADVIW
                     SIGGNPYETQTNYFLAHWVPNLQGGTVDKKKQRFPGETVAAGKAIFVDPRRTVSVDVA
                     EQVAGKDNVLHLDIQPGTDIALFNGLFTHVVEQGWIDRDFIAKHTDGFDAAVATNRMS
                     LQECSQITGVPADKLRKAAEWAYKPKASGHPPRACHTYEKGIIWGNDNYLIQSALVDL
                     VLATHNVGRRGTGVVRMGGHQEGYTRPPYPGDSKIYVDQELINGKGKMMTWWACNNFQ
                     TSNNAQRLREVVLERSQIVKSAISKTRGASAEELVDVIYQACNSGGLFVASIDLYPTK
                     LTEAAHMLLPAAHPGEMNLTSMNGERRMRLSEKFMDPPGAAKADCLIAAMIANTVKAM
                     YQKEGNAEMVKRFSGFDWKTEEDAFNDGFRRAGQPGAGPIDSQGGGTGHIVTYERLRA
                     MGNNGVQLPAQAWKGGKLVGTEMLYTDGKFDTGNGRAQFKSSTWPGLPKTVADQKAKY
                     KFWINNGRVNEVWQTGYHDKYNAFVNGRWPMAFLEMNPDDARSLGVAAGDVVEVFNDF
                     GSTYAMAYPDATAKRGQTFMLFGYFNGVAGDVTTDWTDRNIVPYYKGTWADVRRVGTL
                     AEFKRTVSLKSRHYKGA"
     gene            complement(2054..2821)
                     /locus_tag="VD791_09480"
     CDS             complement(2054..2821)
                     /locus_tag="VD791_09480"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXV08249.1"
                     /translation="MNRLQSKVAAVTGAALGLGRAIAVRMAEEGASVAVLDVLDEAGQ
                     GLAVELVGRGLAARFWHCDVSSESEVARVIGDVARHFGHLNVLVNNAGVAGVNKPTHE
                     LTEAEWDWVQAINVKGVFFCTKHAIPHLRRAGGGSIINLSSIYGLVGAPDVPPYHASK
                     GAVRLMTKTDAMLYAPDRIRVNSIHPGFIWTPMVQKHLSAMGDVEAGRKAADALHPLG
                     HMGEPDDVAWGAVYLASEEAKFVTGAELVIDGGYTAH"
     gene            3260..3754
                     /locus_tag="VD791_09485"
     CDS             3260..3754
                     /locus_tag="VD791_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXV08250.1"
                     /translation="MAEQSKAEVQPAKSGELQKAPSRALRPFEEMFDEFFGRGWLRPF
                     RFERMPELSAAWEGRLPKLDVIDRDNEVLVRAEVPGVKKEDLEISVTGDLFTIKGQTK
                     REEKEEKGDYYRCEVSQGSFSRTVTLPAMVDEAGAKAQLKDGMLEVTLLKTEKSKKRA
                     IRID"
     gene            3781..3957
                     /locus_tag="VD791_09490"
     CDS             3781..3957
                     /locus_tag="VD791_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08251.1"
                     /translation="MPWSDERYPPAMAHLSPPVRAKAIEIANALLEQGRDEGFCIRVA
                     IARALAWARRRGLE"
     gene            complement(3982..5268)
                     /locus_tag="VD791_09495"
     CDS             complement(3982..5268)
                     /locus_tag="VD791_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08252.1"
                     /translation="MSAIVERRPLPVGRLNRKCFCIGADLDGLRAWLHRDLARRGLVR
                     PVVETHPHLFSALPVFVSREHVMGMRSVIAAVESVMPLPAYRAAVLDRAPAVARFAPG
                     PRGVFLGYDFHLADDGPRLIEINTNAGGALLNVELGRAQRACCEEVRGLMTGPLDIGS
                     LEQRLHAMFLEEWRLARGDAQLARIAIVDQAPAQQYLFPEFLLFQRLFEAHGVQAVVA
                     DPAQLDWREGVLSHDGARVDLVYNRLTDFYFEAPEHAALREAYLSGAVVVTPHPRAHA
                     LYADKRNLALLTDEQRLRAWGVPRETIATLQSGIPATRPVDPADAERLWSERKALFFK
                     PACGFGSRGSYRGDKLTRGAFAEILEGDYVAQALVPPSERLLKEADTPQALKVDLRNY
                     VYAGEVQLVAARLYQGQTTNFRTPGGGFAPVFYPPG"
     gene            complement(5265..6512)
                     /gene="arsB"
                     /locus_tag="VD791_09500"
                     /pseudo
     CDS             complement(5265..6512)
                     /gene="arsB"
                     /locus_tag="VD791_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013885.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
     gene            6574..6948
                     /locus_tag="VD791_09505"
     CDS             6574..6948
                     /locus_tag="VD791_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXV08253.1"
                     /translation="MEIKEATIALAALAQETRLAIFRLLVEAGPEGVAAGRISEALEV
                     PPATLSFHLKELAHAGLAHARPESRFVFYSVDFERMAALMTFLTRNCCRGMPEECLTT
                     VETALARCCPAPAKRQPKRTRP"
     gene            6945..7430
                     /locus_tag="VD791_09510"
     CDS             6945..7430
                     /locus_tag="VD791_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009693515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HXV08254.1"
                     /translation="MKRFHVHVSVDDLDANIRFYSTVFGAAPSVHRDDYAKWMIDDPR
                     VNFAISKRGAAPGLDHLGLQVETDEELHALRGQVRQAEVAALDHTGAACCYAKSDKYW
                     ITDPQGIAWETFHTLDVIPVFGEDNRAAQPLEANASCCAPSTKPARILVKAVTDERCC
                     G"
     gene            7442..7942
                     /locus_tag="VD791_09515"
     CDS             7442..7942
                     /locus_tag="VD791_09515"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HXV08255.1"
                     /translation="MDRKAFNVLFLCTGNTARSIMAEVMLNAMGQGRFRAYSAGSHPK
                     DAINPYTIELLKKNRLPTEGLRTKDWGEFARAGAPPLDFVFTVCDQAAGEVCPVWPGQ
                     PMTAHWGIADPAAADGGDDDKRRAFFKAYNQLQHRLSIFVSLPMDKLDRLTLQRRLDE
                     IGKTAD"
     gene            complement(7950..8849)
                     /locus_tag="VD791_09520"
     CDS             complement(7950..8849)
                     /locus_tag="VD791_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXV08256.1"
                     /translation="MQTDRILVATDLSAEAAWAEERGAMLARSAGARLDLVHVVADTL
                     LAAWRAAMLGQDSDVLLSDARQQLSRATQRLRDDVQIDVESQVVTGTPHRAVLDTADR
                     LGSRLIVAGAHGRHPLHTLILGSVTEKLLYRTERPLLITKRQPREGYGHVLAAVDFSP
                     PAAAAVRLASELAAGAAFTLYHAVESPFEGMLRIAGVEREKVEAHRRETIQDAEREMQ
                     RFVTTCGLPSSLAARRVEYGYPTALIEAALQAHGPDLLVLGKHGRSAVERWMVGSVTA
                     HMTRVAQCDVLVVPMSDGAVQSG"
     gene            complement(8849..11566)
                     /locus_tag="VD791_09525"
     CDS             complement(8849..11566)
                     /locus_tag="VD791_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HXV08257.1"
                     /translation="MTRGRWHALDWEDVLRALRSDRNGLTAAEAERRLRRFGPNRLAP
                     PARRSAWQRFLLQFHNVLIYVLLAAGVITGALGHWVDSGVIFGVVLINAIIGFVQEGK
                     AEAAIAAIRKMLSLAAHVLRDGRRVTVPAEQLVPGDLVLLESGDRVPADLRLTEVKSL
                     RIEEATLTGESEPVEKSPQPVAEDAELGDRGCMAYAGTLVTFGQARGVVVATGPSTEI
                     GRISRMLEEVEEVSTPLLRKIGAFGRRLTGAILAFAAFTFAFGVWVRGYSAAEMFLAV
                     AGIAVAAIPEGLPAIMTIALAIGVQRMARRNAIVRRLPAVETLGSVTVICSDKTGTLT
                     RNEMTVQRVVMPLYELDVGGVGYAPEGGFSSADRDVDPVRDPALAELARVALLCNDAA
                     VTRQGEQWVLEGDPTEGALHVLGLKAGLDPHLEAERLPRTDVIPFESQHRFMATLHHD
                     HSGHGLILLKGAPERVLDLADRERRDGSDVALERERWERAVEDCARGGLRVLALAVKP
                     APAGQRGLTYADVEAGGFTLLGLTGMIDPPREEAIAAVRRCRSAGIYVKMITGDHAAT
                     AAAVAEQLGLRGGRALAGVQLDSLDDAGLAQAVPATDVFARASPEHKLRLVQALQANG
                     AVVAMTGDGVNDSPALKRADVGVAMGLKGTEAAKEASDMVIADDNFASIAHAVEEGRT
                     VYDNIKKFLVFMLPTNGGEAGMLVVAILAGLTLPITPVQILWVNMITAITLGLALAFE
                     PAEPDTMSRAPRPPAEPLLNGFLVWRIFFVTVMLVAGGLGLFLWEHHRDGATLEEART
                     IAVNLLVMGELVYLFNSRFLLRSALSREGLAGNRYALAAVAVLIPLQLAFNYLPPMQR
                     LFGTAGLDVKAWLYVCAFGLALFLIVEAEKALLRYWNSTRGPAPSLEGV"
     gene            complement(11699..12259)
                     /locus_tag="VD791_09530"
     CDS             complement(11699..12259)
                     /locus_tag="VD791_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08258.1"
                     /translation="MAHSRTVAKKLLNASELDLFDAGRREGIGTLDAVQLRARIARAR
                     RLRDKYRDLYRRQRVSTRARTGTKSGARGDADLRTGQKGEIFGELVGRFEKRLARIEA
                     AEQRVRMQAVKRARRATQSAARRTAAAPAGAVKPLVPKARTGFMSPAAKSADTRRRLV
                     QTGRVAVQAHTSSRGRRNQAKRDRRS"
     gene            complement(12328..>12698)
                     /locus_tag="VD791_09535"
     CDS             complement(12328..>12698)
                     /locus_tag="VD791_09535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXV08259.1"
                     /translation="ADNFKHVNDRFGHDQGDAVLRAVADALEGNVRDTDCVARLGGDE
                     FVVLFPEADCRAAAAAIGHLQAQLLREMQRNGWPVTFSMGAHTFDSPPESVEALIKAA
                     DALMYDAKRDGGRRIEHRCS"
BASE COUNT         2223 a         4454 c         4179 g         1842 t
ORIGIN      
        1 gcgatccgga ccccgctggt tcgcatccac aaccggccgg cctacaactc cgagtgccac
       61 gccacgcgcg agatgggcat cggcgagctg aacaacagct acgaggacgc cgagctcgcc
      121 gacgtcatct ggtcgatcgg cggcaacccc tacgagaccc agaccaacta cttcctggcg
      181 cactgggtcc ccaacctgca gggcggtacg gtggacaaga agaagcagcg cttcccgggt
      241 gagacggtgg ctgccggcaa ggcgatcttc gtcgatccgc gccgcaccgt ctcggtggac
      301 gtcgccgagc aggtcgctgg caaggacaac gtcctgcatc tggacatcca gccgggaacc
      361 gacatcgccc tgttcaacgg cctgttcacg cacgtggtcg agcaaggctg gatcgatcgc
      421 gacttcatcg ccaagcacac cgacggtttc gacgccgcgg tcgcgaccaa ccgcatgagc
      481 ctgcaggagt gcagccagat caccggcgtg ccggcggaca agctgcggaa agccgcggag
      541 tgggcctaca agcccaaggc atccggtcac cctccgcgcg cctgccacac ctacgagaag
      601 ggcatcatct ggggtaacga caactatctg atccagtcgg ccttggtcga cctggtgctc
      661 gccacgcaca acgtcgggcg gcgcggcacg ggtgtggtgc gcatgggcgg tcaccaggag
      721 ggctacacgc gcccgcccta ccccggcgac agcaagatct acgtcgacca ggagctgatc
      781 aacggcaaag gcaagatgat gacctggtgg gcgtgtaaca acttccagac cagcaacaac
      841 gcgcagcgcc tgcgcgaggt ggtgctggag cgcagccaga tcgtgaagtc cgcgatcagc
      901 aagacgcgcg gcgcatcggc ggaagagttg gtggacgtca tctaccaggc ctgcaacagc
      961 ggcgggctat tcgtcgccag catcgacctg taccccacca agctcaccga ggccgcgcac
     1021 atgctgctgc cggccgcgca tcccggcgag atgaacctta cgtcgatgaa cggcgagcgc
     1081 cgcatgcgcc tgtcggaaaa gttcatggac ccacccggcg cggccaaggc cgactgcctg
     1141 atcgcggcca tgatcgccaa cacggtcaag gcgatgtacc agaaagaagg caacgccgag
     1201 atggtcaagc gcttctccgg cttcgactgg aagaccgagg aggacgcctt caacgacggc
     1261 ttccggcgcg ccggacagcc gggcgcggga ccgatcgaca gccagggcgg aggcacgggc
     1321 cacatcgtca cctacgagcg gctgcgcgcc atgggtaaca acggcgtgca gctgccggcg
     1381 caggcgtgga agggcggcaa gctcgtcggc accgagatgc tctacaccga cggcaagttc
     1441 gacaccggca acggccgcgc ccagttcaag tcttccacct ggcccggcct gccgaagacg
     1501 gtcgccgacc agaaggccaa gtacaagttc tggatcaaca acggccgcgt caacgaggtg
     1561 tggcagaccg gctaccacga caagtacaac gccttcgtga acgggcgctg gccgatggcc
     1621 ttcctggaga tgaacccgga cgacgcgagg tcgctgggtg tggccgcggg ggacgtcgtg
     1681 gaggtgttca atgacttcgg ctccacttac gcgatggctt accccgacgc caccgccaag
     1741 cgcggccaga ccttcatgct gttcggctac ttcaacggcg tggcgggcga cgtcacgacc
     1801 gattggaccg accgcaacat cgtgccctac tacaagggca cctgggcgga cgtgcgccgc
     1861 gtgggcacgc tggcggaatt caagcgcacg gtcagtctca agagccggca ctacaaaggc
     1921 gcatagctgc aagcaccacg ggtaggacga acctttgccg ggcggccatc ggagccctcg
     1981 ttccggtggc cgctctcact cgaatacgat cgaagagcgt ccgtccagcg cccgcgcgat
     2041 cgcggtcgcg gcctcagtgc gccgtgtagc cgccatcgat gaccagctcg gcgccggtga
     2101 cgaacttggc ctcctcggag gccagataca ccgcacccca ggcgacgtcg tccggctcgc
     2161 ccatgtgacc gagcggatgc agcgcatcgg cggccttgcg cccggcttcc acgtcgccca
     2221 tcgcactcag gtgcttctgc accatcgggg tccagatgaa gccgggatgg atcgagttca
     2281 cccggatccg atcgggcgca tacagcatcg cgtcggtctt ggtcatcagc cgcaccgcgc
     2341 ccttggacgc gtggtagggc ggtacgtcgg gcgcgcctac cagtccatag atggacgaca
     2401 ggttgatgat gctcccgccc ccggcgcggc gcaggtgcgg gatcgcgtgc ttggtgcaga
     2461 agaacacgcc cttgacgttg atcgcttgca cccaatccca ctccgcctcg gttagctcgt
     2521 gcgtgggctt gttgaccccg gccacccccg cgttgttgac cagcacgttc agatggccga
     2581 agtgtcgtgc cacgtcgccg atcacgcgtg caacttccga ctcgctgctc acgtcgcagt
     2641 gccagaagcg cgcggcaagc ccgcgcccga ccagttcgac tgcgagccct tgtccggcct
     2701 cgtcgagcac gtccagcacc gccacggacg cgccctcctc ggccatccgc accgcgatcg
     2761 cacggcccaa gcccagcgcc gccccggtaa ccgccgcaac cttgctttga agtcgattca
     2821 tgccttcccc cgctgtccta ggtacacggg cattctgcaa ttccaggcca gactgccgct
     2881 tgacccacct caagacaaag tccggtcgcg acggatgtcg tacacgacga tgcgcacgtc
     2941 aggccggcgc tcacgcaacg cgtcgagata ggcgctggcc tcggcgcagt acgggcatcg
     3001 ggcgcaggta cgaacccgcc gccgcggaaa ctggccgtcc ggcctcggta caggcgcgcg
     3061 ggtaccggtc gcgggtcgct attctagaca caaccgggag gccgatcgaa ctcacggccg
     3121 gcatcgggcc ttgcggcaga tcaaaaccgg gacccgggtt gccgggcaga atcgcgttca
     3181 cgcggctgac gccggcgggt gggcgctgag gcctgctgac gggccgtatc gcgaaccttg
     3241 cgcatacagg agatacgaaa tggctgagca atccaaggct gaagtccagc ccgcgaagag
     3301 cggggaattg cagaaggccc cttcccgggc gctacgcccg ttcgaagaga tgttcgacga
     3361 gtttttcggc cgtggctggc tgcggccgtt ccgcttcgag cgcatgccgg agctgtcggc
     3421 agcatgggaa ggccggttgc cgaagctcga cgtcatcgac cgcgacaacg aagtgctggt
     3481 gcgggccgag gtcccgggcg tgaagaagga ggatctggag atctcggtga ccggtgacct
     3541 gttcaccatc aagggccaga cgaagcgcga agagaaggaa gagaagggcg actactatcg
     3601 ctgcgaggtg tcgcagggca gcttctcgcg caccgtcacc ctgcccgcga tggtggacga
     3661 ggcaggggcg aaagcgcagc tgaaggacgg catgctggag gtcacgctgc tcaagaccga
     3721 gaaatcgaag aagcgcgcga tcaggatcga ctgaggccgc gcgtcgcggg cgggccgccc
     3781 atgccgtgga gcgacgagcg ttacccgccc gcgatggcgc acttgtcccc tccggtgcgc
     3841 gccaaggcga tcgagatcgc caacgcgttg ctggaacagg ggcgcgacga ggggttttgc
     3901 attcgcgtag cgatcgcccg cgcgcttgcg tgggcccgcc gccgaggtct cgaatagcac
     3961 gtcgaagacg gccggagtcg gtcagccggg cggatagaac accggtgcga acccgcctcc
     4021 gggcgtgcgg aaattggtgg tctggccctg gtacaagcgc gccgccacca gctgcacttc
     4081 gccggcgtag acgtagttgc gcagatccac cttcaacgcc tgcggcgtgt ccgcctcctt
     4141 gagcaagcgc tcgctcggcg gcaccagcgc ctgcgccacg tagtcgcctt ccaggatctc
     4201 cgcgaaagcc ccgcgcgtga gcttgtcgcc gcgataggag ccgcggctgc cgaaaccgca
     4261 agccggcttg aagaacagcg ctttgcgctc gctccacagg cgctcggcgt cggcagggtc
     4321 gaccggccga gtggctggaa tcccggactg cagcgtggcg atggtctccc gcggcacacc
     4381 ccaggcgcgc aggcgctgct cgtcggtcag gagcgccagg ttgcgcttgt ccgcatacag
     4441 cgcatgggct cgcgggtgcg gcgtcaccac gacggcgccg gaaagatacg cctcgcgcag
     4501 cgcggcgtgc tcgggcgctt cgaagtagaa gtcggtgagg cggttgtaca ccaggtcgac
     4561 tcgcgcgccg tcgtggctca gcacaccctc acgccaatcg agctgcgcgg ggtcggccac
     4621 caccgcctgc acgccgtgcg cttcgaacag gcgttggaac agcaggaact cggggaacag
     4681 gtactgctgc gccggcgcct ggtccacgat ggcgatgcgc gcgagctgcg catcgccgcg
     4741 ggcgagccgc cactcttcga ggaacatggc atgcagccgc tgttcgagag agccgatatc
     4801 cagcggaccg gtcatcaggc cgcgcacttc ctcgcagcag gcgcgctgag cacggccaag
     4861 ttccacgttg agcaaggcgc ccccggcgtt ggtattgatc tcgatcaacc gcggaccgtc
     4921 gtccgccagg tggaagtcgt agccgaggaa cacgccacgc ggccctggcg cgaatcgggc
     4981 gaccgccggc gcgcgatcca ggaccgccgc ccgatacgcc gggagcggca tcaccgactc
     5041 caccgcggcg atcaccgaac gcatccccat cacatgctcg cgcgagacga acaccggcag
     5101 cgccgagaac agatgcgggt gcgtttccac caccggccgg accagcccgc gccgcgcaag
     5161 gtcccggtgc agccaggccc gcagcccatc caggtcggcg ccgatgcaga agcacttgcg
     5221 gttgagccgg ccgacgggca aaggtctgcg ttcgacgatc gcgctcatgg gcgacccact
     5281 cgcattgcca cgttgcccga gaccgcttgc ggcgactgct ccggaaacca gtgcgcagtg
     5341 cgcctgcaaa gctccaccag catcagcatc accggaactt cgatcagcac ccccacgacg
     5401 gtggcgagcg cgaccccgga ggagagcccg aacagcatca ccgcggtggc gatagcgacc
     5461 tcgaagtggt tcgaggcgcc gatcatggcg gcgggggccg catcggcgta cgacagcttg
     5521 agcagccgcg ccaggccgta gccgagcgcg aagatgagca cggtctgcac caacagggga
     5581 acggcgatcc acagaatggt cagcgggctg gccgcgatga cctcgccctt gaaggagaac
     5641 agcagcacca gcgtgacgag cagcgccgcg atggtagccg gagtcaggac gtgcaggaac
     5701 ctgtccttga accaggtttc gcccttggcg gccacgatcc acttgcggga gaagtagccg
     5761 gccaccagcg gcagcgcgac gtagacgccg atcgagagca acaacgccta ccaaggtacc
     5821 ggcaggcgcc ccacgccgag caggtatccg ccgagcacgc cgtacagcac cagcatggtc
     5881 aacgagttga gcgccaccat caccagcgtc agcccgtcgt tgccacgcgc gaggtatccc
     5941 cacaccagaa ccatggcggt gcagggcgcg atgcccagca ggatgcagcc ggccaggtag
     6001 ctgcgccaca gcggcacctc cagcatcttg acgccgtcgt gcatgaccac ggtgcccgca
     6061 ccgttcatca ttccgaccga caggtcgagc ccgaacggca tcttgaccag atcggtcgca
     6121 tcctcgccga tgaacgcgcg gaacagcacg cccaggaaca gcacggcgat ggcgtacatg
     6181 gtgaacggct tcacccccca gttgacgaac agcgtgagca gcaccggctt cgcgctgcga
     6241 cccgccctga tgaccgaggc gaagtcgatt ttcaccatga tgggatacat catgaagaac
     6301 aggcacaccg cgatcggtat cgagagcacg ggtgcgccgt ccacgtacaa ggccatccgg
     6361 tccaggctgc gggccaaccc gggggcggtc ttgcccagca ggatcccgcc gaggatgcac
     6421 agccccaccc agagggtcag gtagcgctcg aacgggctgc gcatcgtgcg cggctcggga
     6481 tgcggccggg ccgcggttgc aagcgtcgtc atcgaagggg cggtgtcgtt ggccggggtt
     6541 gacaggctac tatattttca caagaatgga aacatggaaa taaaggaagc cactattgcc
     6601 ctcgccgcgc tggcgcagga aacgcggctc gccatcttcc ggctgctggt cgaggccggc
     6661 ccggagggcg tcgccgccgg ccggatctcg gaagcgctcg aggtgccgcc ggccactctc
     6721 tcctttcacc tgaaggagtt ggcgcacgcg ggcctggcgc atgcccggcc ggaaagccgg
     6781 ttcgtgttct acagcgtgga cttcgagcgc atggccgcgc tgatgacctt cctcacccgc
     6841 aactgctgcc gcggcatgcc cgaggaatgc ctgaccacgg tggaaaccgc cctagcccgg
     6901 tgttgccccg cgcccgccaa acggcaaccc aagaggaccc gcccatgaaa cgctttcacg
     6961 tgcacgtctc ggtcgacgac ctcgacgcca acatccgctt ctactcgacc gttttcggcg
     7021 ccgctcccag cgtgcaccgg gacgactatg ccaagtggat gatcgacgat ccgcgggtga
     7081 acttcgccat ctccaagcgc ggcgcggcgc cggggctgga ccacctgggc ctgcaggtcg
     7141 aaacggacga ggaactgcat gcgctgcgcg gccaggtacg gcaggctgag gtcgccgcgc
     7201 tggaccacac cggcgccgcc tgctgctatg caaagtccga caagtactgg atcaccgacc
     7261 cacaagggat cgcctgggaa acgttccaca cgctggatgt cattcccgtg ttcggcgagg
     7321 ataaccgcgc tgcccagccg ctcgaggcga acgcgagttg ctgcgcgccg tcgacgaagc
     7381 ctgcgcgcat cctggtgaag gccgtaaccg acgagcgctg ctgcggatga ggggcggggc
     7441 catggaccgc aaagccttca acgttctgtt cctgtgcacc ggcaacaccg ctcgcagcat
     7501 catggccgag gtgatgttga acgccatggg tcagggccgc ttccgggcct acagcgcggg
     7561 cagccacccg aaagacgcga tcaaccccta caccatcgag ctgctgaaga agaaccgctt
     7621 gcccaccgaa gggttgcgca ccaaggactg gggtgaattc gcgcgcgcgg gcgcgccgcc
     7681 gctcgacttc gtcttcaccg tgtgcgacca ggccgccggc gaggtctgtc cggtctggcc
     7741 cgggcagccg atgacggcgc actggggcat cgcggacccg gcggccgccg acggcggcga
     7801 cgacgacaag cgcagagcct tcttcaaagc ctacaaccag ctgcagcacc ggttgtcgat
     7861 cttcgtcagc ttgccgatgg acaagctcga ccgcctgacg ctgcagcggc ggctggacga
     7921 gatcggcaag accgcggact gagcgggcac tacccggact gcaccgcgcc gtcggacatc
     7981 ggcaccacca gcacatcaca ctgggccacg cgcgtcatgt gggcggtcac gctgccgacc
     8041 atccagcgct cgacggcgga gcgcccgtgc ttgcccaaga ccagcaggtc cggaccgtgt
     8101 gcttgcagcg cggcttcgat cagcgccgtc ggataaccgt actcgacgcg ccgcgcggcc
     8161 agggaggaag gcaacccgca ggtcgtcaca aagcgctgca tctcgcgctc ggcgtcctgg
     8221 atcgtttccc ggcgatgggc ctccaccttc tcccgctcca caccggcgat ccgcagcatg
     8281 ccctcgaatg gtgactccac ggcgtggtac aacgtgaatg ccgcgcctgc ggccagctcg
     8341 gatgccagcc gcaccgcagc cgcggcgggc ggcgagaaat ccaccgccgc caggacgtgg
     8401 ccgtacccct cgcgcggctg ccgcttggtg atgagcaggg gccgctcggt gcgatagagg
     8461 agcttctcgg tcaccgagcc gaggatcagg gtgtgcagcg ggtgacggcc atgcgcaccg
     8521 gccacgatca agcggctccc caaccggtcc gccgtgtcga gcaccgcacg atgaggcgtg
     8581 cccgtcacca cctgggattc gacgtcgatc tgcacatcgt ctcgaagacg ttgcgtggcg
     8641 cgcgacagct gctgtcgcgc atcgctcagc agcacgtcgc tgtcctggcc gagcatcgcc
     8701 gcgcgccatg ccgcgagcag ggtgtcggcc accacgtgca ccagatccag ccgcgcgccc
     8761 gcgctgcgcg ccagcatggc cccgcgctct tcggcccagg cggcctcggc ggacagatcg
     8821 gttgcaacca ggattcggtc ggtctgcatc acacgccctc caggctcggc gccgggccgc
     8881 gggtagaatt ccagtaccgc agcagcgcct tctcggcctc cacgatcagg aacagggcga
     8941 gaccgaacgc gcacacgtac aaccacgcct tgacatccag tcccgccgtg ccgaacaggc
     9001 gctgcatcgg cgggaggtag ttgaacgcga gctgcagcgg gatcaggacg gccacggccg
     9061 ccagcgcgta gcggttaccg gccaggccct cacgggatag cgcgctgcgc agcaggaagc
     9121 ggctgttgaa cagatacacc agttcgccca tcaccagcag gttcacggcg atggtgcgcg
     9181 cctcctccag cgtggcgccg tcgcgatggt gctcccacag gaacaggccc aggccgccgg
     9241 ccaccagcat caccgtgacg aagaagatgc gccacaccag aaacccgttc agcagaggct
     9301 ccgccggtgg tcgcggcgct cgcgacatgg tgtccggttc ggccggctcg aacgcaagcg
     9361 ccagccccag cgtgatcgcg gtgatcatgt tgacccacag gatctgcacc ggcgttatcg
     9421 gcagcgtcag gccggcgagg atcgccacca ccagcatgcc ggcttcgccg ccgttggtgg
     9481 gcagcatgaa cacgaggaac ttcttgatgt tgtcgtagac cgtgcgccct tcctccacgg
     9541 cgtgcgcgat ggaggcgaag ttgtcgtccg cgatcaccat gtcggaggct tccttggccg
     9601 cctcggtgcc cttcagcccc atcgccacac ccacgtcggc gcgcttgagc gccggcgagt
     9661 cgttgacccc gtcgccggtc atcgccacca cggcaccgtt ggcctgcagc gcctgcacca
     9721 gccgcagctt gtgctccggg ctggcgcgcg cgaacacatc cgtggcgggc accgcctgcg
     9781 ccagacccgc gtcgtccagg ctgtccagct ggacgcccgc cagcgcgcgc ccgccgcgca
     9841 agcccagctg ctcggccacg gcggccgcgg tcgccgcgtg gtcgccggtg atcatcttca
     9901 cgtagatgcc cgccgaccgg caacgtcgca ccgcggcgat cgcctcctcg cgcggcggat
     9961 cgatcatgcc ggtgaggccg aggagcgtga agcctccagc ctctacgtcg gcataagtca
    10021 gaccgcgctg accggccggt gcgggcttca cggcgagggc gaggacgcgc aggccgccgc
    10081 gcgcgcaatc ctcgacggcg cgctcccagc gctcgcgctc gagcgccacg tcgctgccgt
    10141 cgcggcgctc gcggtcggcc agatcgagca cgcgctcggg tgcacctttc aacaggatca
    10201 gcccgtgccc gctgtgatcg tgatgcagcg tcgccatgaa gcggtgctgc gactcgaacg
    10261 ggatgacgtc ggtgcgcggc agccgctcgg cctccagatg cggatccaga ccggccttga
    10321 gccccagcac gtgcagggcg ccctcggtgg gatcgccctc gagcacccat tgctcgccct
    10381 gccgcgtcac cgcggcgtcg ttgcacagca gcgcgacgcg cgcgagctcg gccagggccg
    10441 ggtcgcgcac cggatcgacg tcgcggtccg cgctgctgaa gccgccctcg ggagcgtagc
    10501 ccacgccgcc cacatcgagc tcgtacagcg gcatcaccac gcgctgcacg gtcatctcgt
    10561 tgcgcgtcag ggtcccggtc ttgtcggagc agatcaccgt caccgatccc agcgtttcga
    10621 ccgccgggag gcgccggacg atggcgttgc gacgggccat gcgctgcaca ccgatcgcga
    10681 gcgcgatggt catgatggcc ggcagcccct cggggatcgc cgccaccgcg atgccggcca
    10741 ccgccaggaa catctcggcc gccgagtagc cgcgcaccca tacgccgaaa gcgaaggtga
    10801 acgccgcgaa cgcgaggatg gcgccggtca gcctgcgccc gaacgcaccg atcttgcgaa
    10861 gcaggggtgt gctgacctcc tccacctcct ccagcatgcg gctgatgcgg ccgatctcgg
    10921 tggacggccc ggtcgccacc accacgccgc gcgcctggcc gaaggtcacc agcgtccccg
    10981 cgtaggccat gcagccccga tcgccgagct ccgcgtcctc ggcgaccggc tgcggcgact
    11041 tctccaccgg ctcggactcg ccggtcaggg tcgcctcctc gatccgcagg ctcttcacct
    11101 cggtcaaacg cagatcggca ggcactctgt cgcccgactc gagcaggacg aggtcgcccg
    11161 gaaccagctg ctcggcaggc accgtgacac gacggccgtc ccgcagcaca tgcgccgcga
    11221 gagagagcat cttgcggatg gcggcgatgg cggcctcggc cttgccctcc tgcacgaagc
    11281 cgatgatcgc gttgatcagc accacgccaa agatcacgcc ggaatccacc cagtgcccca
    11341 gggcgccggt gatgacaccg gccgcgagca atacgtaaat caggacgttg tggaactgca
    11401 gcaggaagcg ctgccacgcg ctgcgccgcg cgggcggggc caggcgattg ggcccgaagc
    11461 gccgcaggcg gcgctcggcc tcggcagcgg tcagaccgtt gcggtcgctg cgcaacgcgc
    11521 gcaagacgtc ctcccagtcg agcgcgtgcc agcggccccg ggtcatggcg gcatcgaact
    11581 cgcctccgtc cgcttcggct gcggccactg cagtttctcc ttcgcctcgc ataatcgtgc
    11641 ggtctgggtg aagcttacgg attctgtccc ccagtcgcga ctgtcggccc tgccgggttc
    11701 acgaacgccg atcgcgcttg gcttgattgc ggcgcccacg gctgctcgtg tgggcctgca
    11761 ccgcgacgcg gcccgtctgg accagccggc gccgggtgtc ggcgctcttc gctgccgggc
    11821 tcatgaagcc ggtgcgggct ttcgggacga gcggcttgac cgcacccgcg ggcgccgcag
    11881 cggttcgtct cgcagcggac tgggttgcac gccgggcgcg cttgaccgcc tgcatccgca
    11941 cccgctgctc ggccgcctcg atccgagcca gacgtttctc aaaacgcccc accagctccc
    12001 cgaagatctc ccctttctgc ccggtgcgca ggtcggcgtc cccgcgcgcg ccgctcttgg
    12061 tgccggttcg ggcccgcgtc gatacgcgct gccggcgata caggtcccgg tacttgtcgc
    12121 gcagacggcg cgcacgcgca atccttgcgc gcaactgcac ggcgtccagc gtgccgatcc
    12181 cctcgcggcg gccggcgtcg aacagatcga gctccgaggc gttgaggagc ttcttggcaa
    12241 cggtgcggct atgagccatc tcactctcca ttgatcgaga ctgcaacacg acggtgcacc
    12301 gaggcacagt cgccggcgat cgacaggtca cgagcagcgg tgctcgatcc gcctcccgcc
    12361 gtcgcgcttg gcatcgtaca tgagcgcgtc ggcggccttg atcagcgcct ccaccgactc
    12421 gggcggggag tcgaaggtgt gcgctcccat gctgaacgtc accggccagc cgttgcgttg
    12481 catttcgcgg agcagttgcg cctggagatg gccgatcgcc gcggcggcgg cccggcaatc
    12541 ggcttccggg aacaggacca cgaactcgtc gccccccaag cgcgccacgc aatcggtgtc
    12601 gcgcacgttg ccttcgagcg catctgccac cgcgcgcagc accgcatcgc cctggtcgtg
    12661 tccgaagcgg tcgttcacgt gcttgaagtt gtccgcgt
//
LOCUS       DASPGE010000296         4264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1600294, whole genome shotgun sequence.
ACCESSION   DASPGE010000296 DASPGE010000000
VERSION     DASPGE010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600294"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            672..1976
                     /locus_tag="VD791_09540"
     CDS             672..1976
                     /locus_tag="VD791_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXV08260.1"
                     /translation="MLAAALLCACTTSSSWKASTAASTARVAKAKTVDAPYEREEVNY
                     ATPLGGAAESSDAASSSASAQAASAGTTYGDEGGMQSDAAQHASAAQMPESTAQDGAA
                     GAGSDFEDHAARDEGATSADSAGSTTAGEDARDDALAGTLEDSAGAGSQADAEAGRAE
                     SAASSEAGASGQAGDASDASGAMDTSAGDASAGGAALAADTLAGAPDDHGASMHAGDE
                     TASGRADSAAQGQTAPQADSTAGAGAATAAADSSLDAAAAAAASQQADAARAQEPLIV
                     GVVEAPGKKATRNEEVIPLTLGGLLPMTLGVDGQGEFDFDRAVLREQVKELLDTLVSR
                     LKGAEYDRIEIVGHTDRIGTEDYNQYLSERRAWAVARYLAKQGVPVTKMQVEGRGMQE
                     PVTGAHECDAKDIDRDQLIVCLQKDRRVVISASIRKVDVNVQ"
     gene            complement(2025..2492)
                     /locus_tag="VD791_09545"
     CDS             complement(2025..2492)
                     /locus_tag="VD791_09545"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HXV08261.1"
                     /translation="MAKARLSTDRRRLILSALRLGGASLVSAGAVFAAAGATTAASAR
                     PRFMRRAFELRQMSLERGDHGYGAVVVKDGVVVGEGANAVNTTPDPTAHSEVQAIRDA
                     ARRLGAARLTGCELFTNARPCPMCQMAAYWAGIERIYYGESITDAGAPRYMAC"
     gene            2550..3572
                     /locus_tag="VD791_09550"
     CDS             2550..3572
                     /locus_tag="VD791_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXV08262.1"
                     /translation="MRYRLLGRTGLYVSEICLGAMTYGGKGRWEVVGRLGAAEAQAQI
                     KAAFDAGVNFIDTANVYSEGESEKLVGQALAGLGLPREDLVIATKVRVRMGPAPNRVG
                     LSRAHIMHELDASLRRLRLDHVDLYQIHGVDQVTPLDETFRALEDAVRSGKVRHLGVS
                     NHAAWQIMKALGISAQHDWNRLESVQAYYSIAGRELEREIVPMARDQGLSILAWSPLA
                     GGLLSGKFSAGAAGPEGARRTTFDFPPVDRERVFRCVEAMRPMAQAHGVSVARMALAW
                     VLAQPGVTSVIVGAKTQEQLLDNLAAADLRLSDAELHTLEEVSRIPREYPGWMFERQL
                     ADRLPG"
     gene            3589..>4264
                     /locus_tag="VD791_09555"
     CDS             3589..>4264
                     /locus_tag="VD791_09555"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXV08263.1"
                     /translation="MQRTIPISLVSLAVLLGLAAFAPGAWAQQFPTKAVRVITPFSAG
                     SGPDSVLRLVADKLSKLWGQPVVVENRPGGSGVIALAAANGSAPDGYTLVELDDTHMA
                     LQPHLYADARYDGVRHLVPVATLLKTHFFIVVAANSPWRNVPDLVRAAKARPGELTYG
                     SWFIGSPGHVGAAMLQAATDTSMSHVPFKDMAQLYAAVANGDVAWAFGSAASAGPMVR
                     AGKVQFL"
BASE COUNT          720 a         1431 c         1531 g          582 t
ORIGIN      
        1 ttcgcgccct tcccgacact ggagggcgcg gtctgaactt tcgggtcgag ccggatgttc
       61 tgcggcttga cggtgcagcc gagagcgagc acggcggcga agcaaatcag cggcgacagc
      121 gatcgaatga gcggagaagt caaggaggtc ctcctgcaag tggagcggcg cgcagggcgc
      181 gccaggactc aggaatagca gccggtggcg cgagaagcgc cccgcacgac agggcgtgtg
      241 cgccgggctg cagcggaaag aatggattat attccagaaa actgaaggct gcacatcgcc
      301 tgccgggcgg cgtggtgagg cgcgctgccc tgcgccgaga ccgctcgaaa gcgcgcaccg
      361 ttgggcttga cttgtacgcc tgcggccata tgatttatgc caatgccagt cacaagagac
      421 gacgtgtcgc ctgctggcgg catagtccgc atccgcgagt gtcggcgggc aacgaccggc
      481 gccgaccgga caggccgcgc cagatgcgtt gtcgcaaaaa actcgggcgc gcggtcgcaa
      541 acccgggccc gacacgcact ttgaagcaga agcagggagc cagccgatac aggagacagt
      601 ctatgaacga tcgaaatcgc gcgctgccgg ccccgacgag tctggccaag gccggcatgc
      661 agtggagcgc cgtgctcgcc gccgcacttt tgtgtgcgtg cacgacctcc tcgtcgtgga
      721 aggccagcac ggcggcgtcg acggcacgcg tagcaaaggc gaagacggtc gatgcgccgt
      781 acgagcgtga ggaagtgaac tacgcgacgc cgctgggcgg cgccgcggag tcgtcggatg
      841 cggcctcgag cagcgcgtct gcgcaagccg cgtctgcggg caccacgtac ggcgacgaag
      901 gcggcatgca atccgacgcg gcacagcacg ccagcgccgc acagatgccg gagagcacgg
      961 cacaggatgg agcggcgggc gccggcagcg acttcgaaga tcacgcggca cgcgacgagg
     1021 gcgcaaccag cgcggacagc gccggcagca ccaccgccgg tgaggacgcg cgggacgacg
     1081 ccctggcggg aaccttggaa gacagcgcgg gcgcgggttc gcaggccgac gccgaggccg
     1141 ggagggcaga aagcgctgcg tcgagcgagg cgggcgcgtc cggccaggcg ggcgacgcaa
     1201 gcgacgcttc gggcgccatg gataccagcg ctggggatgc gagcgccggc ggcgccgcgc
     1261 tggcggccga caccctggca ggcgccccgg acgatcacgg cgcgtccatg cacgcgggcg
     1321 acgagacggc gtccgggcga gcggacagcg ccgcgcaggg gcaaacggca ccgcaagccg
     1381 actccaccgc gggggccggt gccgcaaccg cagcggcaga cagcagcctg gacgcggcag
     1441 cggcggcagc cgcaagccag caggcggatg cggcccgtgc gcaggagccg ctcatcgtcg
     1501 gcgtggtcga agcgcccggc aagaaggcga cgcgcaacga ggaggtgatt ccgctgacgc
     1561 ttggcggcct gctgccgatg acgctgggcg tcgacggtca gggcgagttc gatttcgacc
     1621 gcgccgtgct gcgcgagcag gtcaaggaac tgctggacac gctcgtgtcc cggctcaagg
     1681 gcgccgagta cgaccgcatc gagatcgtcg gccacaccga ccgcatcggc accgaggact
     1741 acaatcagta cctgtccgaa cgccgcgcct gggcggtggc ccgctacctc gccaagcagg
     1801 gcgtgccggt caccaagatg caggtcgaag gccgcggcat gcaggagccg gtcaccgggg
     1861 cgcacgagtg cgacgccaag gacatcgacc gcgatcagct catcgtctgc ctgcagaagg
     1921 accgccgcgt ggtgatctcg gcgtcgatcc gcaaggtgga tgtgaacgtg caataggccg
     1981 ctacgccttt gccagccgct cagcagaccg tgtaacccag ccgctcagca ggccatgtaa
     2041 cgtggcgcgc cggcgtcggt gatcgattcg ccatagtaga tgcgctcgat cccggcccag
     2101 taggccgcca tctggcacat cggacagggt cgcgcgttcg tgaagagctc gcaccctgtc
     2161 agtcgtgcag cgcccagacg gcgagcggcg tcacggatcg cttgcacttc gctgtgggcg
     2221 gtcggatcgg gagtcgtgtt gaccgcgttg gccccctcac ccaccaccac gccgtccttg
     2281 acgacgaccg caccgtagcc gtggtcgccg cgctccagag acatctgccg caactcgaaa
     2341 gcgcgtctca tgaatcgcgg gcgcgctgaa gccgcagtcg tggcgccggc cgccgcgaag
     2401 acagcacccg ccgataccag acttgcgcca cccagccgca gtgccgacag gatcagccga
     2461 cggcggtcgg tcgataacct cgctttggcc atgatctgct cctcgctgtt ccgcggctat
     2521 actgccaggt ttccacgagg gagcccgcca tgcgctatcg tctgctcggc cgcaccggcc
     2581 tgtacgtttc ggagatctgc ctgggcgcga tgacctacgg cggcaaggga cgctgggagg
     2641 tggtcggacg cctgggcgcc gccgaggcgc aggcgcagat caaggccgct ttcgacgccg
     2701 gcgtcaactt catcgacacc gccaacgtct actcggaagg cgaatccgag aagctcgtgg
     2761 ggcaagcgct cgccggcctc gggctgccgc gcgaggacct cgtgatcgcc accaaggtgc
     2821 gcgtgcgcat ggggccggct cccaaccggg tcgggctgtc gcgcgcgcac atcatgcacg
     2881 agctggacgc cagcctgcgg cgcctgcgcc tggatcacgt cgacctgtac cagatccacg
     2941 gcgtggacca ggtgaccccg ctggacgaga ccttccgggc gctggaggac gccgtgcgct
     3001 cgggcaaggt gcgccacctc ggggtatcga accacgccgc ctggcagatc atgaaggcgc
     3061 taggcatcag cgcgcagcac gactggaacc ggctggagag cgtgcaggcc tattattcga
     3121 tcgccggccg cgagctcgag cgcgagatcg tgccgatggc gcgcgaccag gggttgagca
     3181 tcctggcgtg gagcccgctc gccggcggcc tgttgagcgg aaagttcagc gccggcgcgg
     3241 ccgggccgga aggcgcgcgc cgcacgacct tcgacttccc gcccgtcgat cgcgagcgcg
     3301 tgttccgctg cgtcgaggcg atgcgcccga tggcgcaggc gcacggcgtg tcggtcgcgc
     3361 ggatggcgct cgcctgggtg ctggctcagc ccggcgtgac cagcgtgatc gtgggcgcga
     3421 agacgcagga gcagctcctc gacaacctcg cggcggccga cctgcggctg tccgacgccg
     3481 agctgcatac cctggaggaa gtcagccgca tcccgcgcga gtaccccggc tggatgttcg
     3541 agcggcagct cgccgaccgc ttgccgggct gagcgccggc gcctcggcat gcaacgtacg
     3601 attcccatct cgctggtctc gcttgcggtc ttgctcgggc tggcggcgtt cgctccgggt
     3661 gcgtgggcac agcagttccc caccaaggcg gtcagggtga tcaccccgtt ctcggccggc
     3721 agtggccccg attcggtgct gcgcctggtc gcggacaagc tgtccaagct ctggggacag
     3781 ccggtggtcg tggagaatcg cccgggagga agcggcgtca tcgccctcgc ggctgcgaac
     3841 gggtcggcgc cggacggcta cacgctggtc gagctggacg acacgcacat ggctctccag
     3901 cctcacctgt acgcggacgc acgctacgac ggcgtgcgcc acctggtccc cgtcgccacg
     3961 ctgctcaaga cccacttctt catcgtggta gcggcgaatt cgccctggcg caacgtgccc
     4021 gatctggttc gggcggcaaa ggcgaggccc ggggagctga cttacggctc ctggttcatc
     4081 ggcagtcccg ggcatgtggg tgccgcgatg ctccaggccg ccacggacac cagcatgagc
     4141 catgtcccgt tcaaggacat ggcgcaactg tacgcggccg tggcgaacgg agacgtcgcc
     4201 tgggcattcg gctccgcggc gtcggcgggc cccatggtcc gggccgggaa agtccagttc
     4261 ctgg
//
LOCUS       DASPGE010000297         4771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_429923, whole genome shotgun sequence.
ACCESSION   DASPGE010000297 DASPGE010000000
VERSION     DASPGE010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429923"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /gene="aceE"
                     /locus_tag="VD791_09560"
     CDS             <1..345
                     /gene="aceE"
                     /locus_tag="VD791_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240791.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HXV08264.1"
                     /translation="WNLLHPEEPQRKSYVERCLEGHEGPAVAATDYMRTFADQIRPYL
                     KRSYTVLGTDGFGRSDFRKELRRFFEVDRYYVSIAALKALADEGKVPRATVAQAIRKY
                     GIDANKPCPWLV"
     gene            409..1752
                     /gene="aceF"
                     /locus_tag="VD791_09565"
     CDS             409..1752
                     /gene="aceF"
                     /locus_tag="VD791_09565"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HXV08265.1"
                     /translation="MQEVLVPDIGDFKDVPIIEVLVKPGDRVKPDDSLVTLESDKATM
                     EVPAPFGGVVQAMKVKVGDRVSQGSLILTLETGEAGTATAGAPAAAAASAPAPAPAAT
                     PSTLAAAWAPVPPAAVASAPAPAVSPAAPTPAVPASMTVDEAGFARAHASPAVRRFAR
                     ELGLDLARVKGTGPKGRVLKEDVQGFVKQALARPAAAGPGLGFALPAMPAVDFAKFGP
                     IEAKPLARIKKISGAYLHRNWVAIPHVTQHDEADITEMEAFRKQMAEEVKAQGIRVTP
                     LAFLMKACVAALQRFPQFNASLSPEGDSLILKQYWHLGVAVDTPNGLVVPVIRDVDKK
                     GVLQLAKELGEVSAKAREGKLSPADMQGGTFSISSLGGIGGSSFTPIINAPEVAILGA
                     SRSVTRPQWRDGGFVPRLMLPLSLSYDHRVVDGAEGARFITFLTGVLSDIRRLVL"
     gene            1749..2417
                     /gene="nadD"
                     /locus_tag="VD791_09570"
     CDS             1749..2417
                     /gene="nadD"
                     /locus_tag="VD791_09570"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HXV08266.1"
                     /translation="MNHAPAIGILGGTFDPIHYAHLRLGEELADRVGLAQVRFVPARV
                     PPHRAAPNVTAEHRLEMTRLATAGNPRFSVDDRECRRQGPSYTLDTLHELRAELGAAR
                     PLCLLMGVDAYLGLTTWSRWPQLYEAAHIVIAHRPGFALDTRAMPEELSQQTASRLTA
                     DAQALQERPGGLVITVDIPSLDISATVIRKLIHAGRSARYLLPDDVLDYIERNRLYKD
                     FDAD"
     gene            2437..2796
                     /gene="rsfS"
                     /locus_tag="VD791_09575"
     CDS             2437..2796
                     /gene="rsfS"
                     /locus_tag="VD791_09575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HXV08267.1"
                     /translation="MAALEEIKARDIAVLDVRKLTSLFDFLIVASGESARQTKALARN
                     VHDKVKAAGGVVLGVEGEQSGEWVLVDLGAVVVHVMQPAVRDYYRLEQLWGGEVPRRL
                     SPLRRPEWATQRGQARP"
     gene            2910..3392
                     /gene="rlmH"
                     /locus_tag="VD791_09580"
     CDS             2910..3392
                     /gene="rlmH"
                     /locus_tag="VD791_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HXV08268.1"
                     /translation="MKLHILAVGHRMPAWVQAGFEEYARRMPREMRLSLKEVRPEPRG
                     SGAPPAAQIERILKAEAERLRGALLHGCLLVALDERGQSLTTERFARQLAEWLQQGRD
                     ACFAIGGADGLDADLKRSAHLMLSVSAMTLPHQLVRVILAEQLYRAACLLQHHPYHRR
                     "
     gene            3394..4032
                     /locus_tag="VD791_09585"
     CDS             3394..4032
                     /locus_tag="VD791_09585"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HXV08269.1"
                     /translation="MQAPDRHIYLASRSLRRRELLRQIGIGFEVLLLREAPGRPVDFD
                     EDPRADERAEAYVERIARHKAMTGWARLSQRRMMRRPVLAADTTVALDGTILGKPADR
                     EEATRFLQRLSGRTHSVHTAVAVAFEERLEVAVSSSEVEFGVLGDQDIRRYVATGEPL
                     DKAGAYAIQGRAAVFVRQVCGSYTGVVGLPLFETSQLLARFGIDPFPARSHE"
     gene            4025..>4771
                     /locus_tag="VD791_09590"
     CDS             4025..>4771
                     /locus_tag="VD791_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HXV08270.1"
                     /translation="MNEEILINVTPQETRVAVIQQGVVQELHVERTTGRGLVGNVYVG
                     VVSRVLPGMQSAFVQIGLDRAAFLHVADIWEHRQNGTEPRPIERLLHDGQRLLVQVIK
                     DPIGTKGARLSTQISVAGRLLVYLPQECHIGISQRIGDEAERAQLRERLQQLLPAGET
                     GGFIIRTMAETATDREMLSDIDYLRRTWAGIQERAQSVTAPTLLYQDLGLGHRVLRDI
                     ANDETERILVDSRETCQKLQAFAQDYTFGFV"
BASE COUNT          750 a         1646 c         1679 g          696 t
ORIGIN      
        1 tggaatctgc tgcaccccga ggagccgcag cgcaagagct acgtggagcg ctgcctggag
       61 ggccacgaag gcccggccgt ggccgccacg gactacatgc gcaccttcgc cgaccagatc
      121 cggccgtacc tcaagcgcag ctacacggtg ctcggcaccg acgggttcgg gcgctccgac
      181 ttccgcaagg agctgcgacg cttcttcgag gtggaccggt actatgtgtc gatcgcggcg
      241 ctgaaggccc tggcggacga gggtaaagtg ccgcgggcga ccgtggccca ggcgatccgg
      301 aagtacggga tcgatgccaa caagccgtgc ccctggctgg tctagaaccg cagcccggga
      361 ccgaccaagc ggccgcgaca acaacgacgg gagacgaccg tgggagcgat gcaggaggtg
      421 ttggtcccgg acatcggcga cttcaaggat gtgccgatca tcgaagtgct ggtgaagccg
      481 ggcgaccggg tcaagccgga cgactcgctg gtcaccctgg agagcgacaa ggccaccatg
      541 gaggtgccgg cgcccttcgg cggcgtggtg caggccatga aagtgaaggt cggcgaccgc
      601 gtgtcgcagg gctcgctgat cctcacgctg gaaaccggcg aggcgggcac cgccacggca
      661 ggcgcaccgg cggcggccgc ggcatctgcg ccggctcccg cgcctgcggc cacaccttcc
      721 acgcttgcgg cggcatgggc tcccgtgccg ccggctgcgg ttgcgtcggc acccgcgccc
      781 gcggtgtcgc cagcagcacc cacaccggcg gtgcccgcat cgatgacagt ggacgaagcc
      841 ggcttcgcgc gcgcgcatgc cagtcccgcc gtgcgccggt tcgcgcgcga gctgggtctc
      901 gatctcgcca gggtgaaggg caccggtccg aagggccgcg tgctcaagga ggacgtccag
      961 ggcttcgtga aacaggcgct ggcgcgcccg gccgcagccg ggcccggcct cggtttcgcg
     1021 ctgcccgcga tgccggcggt ggacttcgcc aagttcgggc cgatcgaggc caagccgctg
     1081 gcgcgcatca agaagatctc cggcgcctac ctgcatcgca actgggtggc gattccccac
     1141 gtcacccagc acgacgaggc cgacatcacc gagatggaag cctttcgcaa gcagatggcg
     1201 gaggaggtca aggcgcaagg catccgcgtg acaccgctcg ccttcctgat gaaggcttgc
     1261 gtggccgcgc tgcagcgctt tccgcagttc aacgcgtcgc tctcccccga gggcgacagc
     1321 ctgatcctga agcagtactg gcatctgggc gtcgcggtgg acacccccaa cgggctggtg
     1381 gtgccggtga tccgcgacgt ggacaagaag ggtgtcctgc agctcgccaa ggagctgggc
     1441 gaagtcagcg ccaaggcgcg cgagggcaag ctctcgccgg ccgacatgca gggcggcacc
     1501 ttctccatct ccagcctggg cggcatcggc ggctcgagct tcacgccgat catcaacgcg
     1561 ccggaagtcg ccatcctcgg cgcttcgcgc agcgtcacca gaccgcaatg gcgcgacggc
     1621 gggttcgttc cgcggctgat gctgccgctg tcgctgtcct acgatcaccg cgtggtggac
     1681 ggcgcggagg gcgcgcgctt catcaccttc ctcaccggtg tgctgagcga catccgccgg
     1741 ctggtgttgt gaaccacgcg cctgcgatcg gcatcctcgg cggcaccttc gacccgatcc
     1801 actacgccca cctgcggctc ggcgaggagc tggccgaccg ggtcggcctg gcgcaggtgc
     1861 gcttcgttcc ggcgcgcgtc ccaccgcatc gcgccgcccc caatgtcacc gccgagcacc
     1921 ggctggagat gacgcggctg gccacggccg gcaacccgcg cttcagcgtc gacgaccgcg
     1981 agtgccgccg gcagggcccg agctacaccc tcgacaccct gcacgagctc cgagcggagc
     2041 tgggcgcggc ccggccgctc tgcctgctga tgggagtgga cgcttacctc gggctgacca
     2101 cctggtcgcg ctggccacag ctctacgaag cggcgcacat cgtgatcgcg caccggcccg
     2161 gcttcgccct ggacacgcgc gccatgccgg aggagctctc ccagcagacc gcatcgcgcc
     2221 tcaccgccga cgcgcaggcc ctgcaggaac gcccgggcgg cctggtgatt accgtggata
     2281 ttccttccct tgacatttcc gccacggtca tccgcaagct gatccacgcc ggccgcagcg
     2341 ctcgctactt gcttcccgac gacgttctcg attacattga acgcaaccgg ctctacaagg
     2401 atttcgatgc agattgagga gattaggaat accgcggtgg ccgccctgga ggagatcaag
     2461 gcacgcgaca tcgcggtgct ggatgtgaga aaactgacat cgttgttcga ttttctgatc
     2521 gttgcaagcg gcgagtccgc gcgccagacc aaggcgctcg cccgcaacgt gcacgacaag
     2581 gtgaaggccg ccggcggcgt ggttctgggc gtcgaaggcg agcagtcggg ggagtgggtg
     2641 ctggtggacc tgggcgccgt ggtggtgcac gtgatgcagc ccgcggtgcg cgactactac
     2701 aggctggagc agctctgggg cggcgaagtc ccgcggcggt tgtcgccgct gcgccgcccc
     2761 gaatgggcta cccagagagg tcaggcccgc ccctgacgcg gccgccgtcg atgcggccgc
     2821 caacctcgac ccgcagccca ctgaaggact cgggccgatg gcgcgctatc atcacgaatg
     2881 atccgggttg agccgcaggc cgcggcgccg tgaagctgca catcctcgcg gtgggtcacc
     2941 gcatgccggc atgggtgcag gccggtttcg aggagtacgc gcggcgcatg ccgcgcgaga
     3001 tgcgcctgtc gctcaaggag gtgcgccccg agccgcgggg cagcggggca ccgcccgcgg
     3061 cgcagatcga gcgtatcctg aaggccgaag ccgagcgcct ccgcggcgcc ttgctgcacg
     3121 gctgcctgct ggtggcgctg gacgagcgcg gtcagtcgct caccaccgag cgcttcgcgc
     3181 ggcagctggc agaatggctg cagcagggcc gcgacgcgtg cttcgcgatc ggcggcgcgg
     3241 acggtctgga cgcggacctc aagcgctcgg ctcatcttat gctttcggtc tccgccatga
     3301 cgcttccgca tcagctggtg cgcgtgatcc tcgccgagca gctctaccgc gcggcctgcc
     3361 tgttgcagca tcacccctat catcgccgct gacatgcaag cgccggaccg ccacatctac
     3421 ctggcttcgc gcagcctgcg ccggcgcgag ctgctgcgcc agatcggcat cggtttcgag
     3481 gtgctgctgc tgcgcgaggc gccgggccgg ccggtggatt tcgacgagga tccgcgcgcg
     3541 gacgagcgcg cggaagccta tgtcgagcgc atcgcccggc acaaggccat gaccggctgg
     3601 gcgcggctca gccagcggcg catgatgcgc cgtccggtgc tggcggcgga caccaccgtc
     3661 gcgctggacg gcacgatcct cggcaaaccg gccgaccgcg aagaagcgac gcggtttctg
     3721 cagcgcctct ccggcaggac gcactccgtg cacaccgcgg tcgcggtggc tttcgaggag
     3781 cgactggagg tggcggtatc cagcagcgag gtggagttcg gcgtgctcgg cgaccaggac
     3841 atccggcgct atgtggccac cggcgagccg ctggacaagg ccggggcgta cgcgatccag
     3901 ggccgggcgg cggtgttcgt gcgccaggtc tgcggcagct acaccggcgt cgtggggctg
     3961 ccgctgttcg agaccagcca gctgctggcg cgcttcggca tcgatccgtt ccccgcacgc
     4021 agccatgaat gaggagatac tgatcaacgt cacgccgcag gagacgcggg tggcggtcat
     4081 tcagcaaggc gtggtgcagg agctgcacgt ggagcgcacc accggccgcg ggctggtcgg
     4141 caacgtctac gtcggcgtgg tgagccgggt gctgccgggc atgcagtcgg cgttcgtgca
     4201 gatcgggctg gaccgcgccg ctttcctgca cgtggccgac atctgggagc accgccagaa
     4261 cggcactgag ccgcggccga tcgagcgcct gctgcacgac ggccagcgcc tgctggtgca
     4321 ggtgatcaag gacccgatcg gcaccaaggg cgcgcggctg tccacgcaga tcagcgtcgc
     4381 cgggaggctg ctggtgtacc tgccgcagga gtgccacatc ggcatctcgc agcgcatcgg
     4441 cgacgaggcc gagcgcgcgc agctgcgcga gcggctgcag cagctgctgc ccgccggcga
     4501 gaccggcggc ttcatcatcc gcaccatggc cgagaccgcc accgaccggg agatgctgtc
     4561 cgacatagac tacctgcggc gcacctgggc gggcatccag gagcgcgccc agagcgtcac
     4621 cgcaccgacg ctgctgtatc aggacctggg cctgggccac cgggtgctgc gcgacatcgc
     4681 caacgacgaa accgagcgca tcctggtgga ctcgcgcgag acctgccaga agctgcaggc
     4741 cttcgcccag gattacacct tcggcttcgt c
//
LOCUS       DASPGE010000298         6087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_313607, whole genome shotgun sequence.
ACCESSION   DASPGE010000298 DASPGE010000000
VERSION     DASPGE010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313607"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VD791_09595"
     CDS             <1..418
                     /locus_tag="VD791_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HXV08271.1"
                     /translation="VGATNGWKMEQVVPSRELVQRIDREMPIEPVQPNYEGEVAERWR
                     YRDGSGEVGFISSVTQAFCRSCTRARLSAEGQLYTCLFAEHGTDFRGLLRGGASDDEI
                     ADLIHRVWTVRADRYSEIRTAETAKARKVEMSYIGG"
     gene            435..1706
                     /gene="glp"
                     /locus_tag="VD791_09600"
     CDS             435..1706
                     /gene="glp"
                     /locus_tag="VD791_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HXV08272.1"
                     /translation="MDKPASLAALAADSDYDPNSMPVAKARDYIRAFLTPITGSERLH
                     IRAALGRVLAEDVLSGIDVPAHDNSAMDGYAFPHADLKSVGETSFAVVGTSFAGKPFA
                     GQVGAGQAVRIMTGGVMPAGADSVVMQERVTASGDTVRIPAGQQEGQNVRLAGADIRR
                     GSVVFRCGQILRPAEIGMLASLGIGEAAVFRKLRVAFFSTGDELVGIGSPLAAGQIYD
                     SNRYTIHGMLTRLGCEVLDLGVIRDDPAAIERAFREASQSADVVITSGGVSVGEADFV
                     KQILNRLGEVLFWKIAMRPGRPLAYGKLGQAHFFGLPGNPVSAMVTFYQFVRDALLVL
                     MGVPEPQPLPTFKALCTSPIKKAPGRTEFQRGILSHAPDGRWTVRVTGDQSSGILSSM
                     SQANCFIVLRDDQGAAPAGTEVDVQVLEGII"
     gene            1727..2275
                     /gene="mobA"
                     /locus_tag="VD791_09605"
     CDS             1727..2275
                     /gene="mobA"
                     /locus_tag="VD791_09605"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HXV08273.1"
                     /translation="MILAGGRGRRMGQVDKGWAELDGRPLVGRVLERFAPQVGQVLIS
                     ANRNRERYAALGHEVIADLMPDHAGPLAGLHAAFAHARCDLIATVPCDSPWLPLDLVQ
                     RLRNALERSSAQVAVARSGGRLHPVFLLCRKTVAGQLEAYLAGGARKAEGWCATLPCE
                     QVDFDDQPEAFRNINTPADLER"
     gene            complement(2293..3372)
                     /locus_tag="VD791_09610"
     CDS             complement(2293..3372)
                     /locus_tag="VD791_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007734540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08274.1"
                     /translation="MVAGAACISISACSTAPLSPYTADTPPLVLVPAQQAGVNDKRAR
                     FREIFCAVLEAHGHDLPDYRPCEEALTRVGTEPAATGKPVDLGPSRRRLIAGIVPGLG
                     WECFADWLQASDSAAAHVRQFGYEFKVLEVGGLSGTSVNARRIRDTVLAMQLGPDEER
                     RLVLIGYSKGAPDLLEAVVGYPEIRGVVAAAVSAAGAVGGSPLANRARQSQADLLRHW
                     PGAKCEKGDGLAVDSLRPGVRRAWLAGNPLPQDFPYYSVVTYPHPDRISAALRGSYDK
                     LSKVDARNDSQLVFYDQVIPGSTLAAYVNADHLALTIPVNRSHPLIASLFATQNAYPR
                     EALLEALLRFVEEDLEALERQPGRP"
     gene            complement(3476..3772)
                     /gene="ftsB"
                     /locus_tag="VD791_09615"
     CDS             complement(3476..3772)
                     /gene="ftsB"
                     /locus_tag="VD791_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HXV08275.1"
                     /translation="MRVLAVVLVILIAAIQTPLWFGKGGWVRVWELDRQLEAQRQSNE
                     RLRARNAALDAEVRDLKTGYDAIEERARSELGMIRQDEVFIQVLEPQRAAPDAQ"
     gene            complement(3782..5065)
                     /gene="eno"
                     /locus_tag="VD791_09620"
     CDS             complement(3782..5065)
                     /gene="eno"
                     /locus_tag="VD791_09620"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HXV08276.1"
                     /translation="MSSIVDVIAREILDSRGNPTVEADVLLESGVMGRAAVPSGASTG
                     EKEAIELRDGDPQRYLGKGVLRAVENVNTEICEAIIGLDAVEQALIDRTLIELDGTDN
                     KARLGANATLAVSLAVAKAAAEDSGLPLYRYLGGAGRMQLPVPMMNVINGGAHANNSL
                     DLQEFMIIPVGAASFAEAMRCGAEVFQALRKLIDGKGMPTTVGDEGGFAPNLPGNEAA
                     LQLIMQAIEGAGYSAGTQVAIGLDCASSEFFKEGRYRIESEGLQLTAAQFADYLGAWV
                     DKYPILSIEDGMAQSDWDGWEILTDRLGDSVQLVGDDLFVTNTRILAEGIRRGVANSV
                     LIKINQIGTLTETFAAIEMAKRAGYTAVVSHRSGETEDTIIADLAVAANTLQIKTGSL
                     SRSDRMAKYNQLLRIEEDLGESASYPGRGAFYQLF"
     gene            complement(5117..5953)
                     /gene="kdsA"
                     /locus_tag="VD791_09625"
     CDS             complement(5117..5953)
                     /gene="kdsA"
                     /locus_tag="VD791_09625"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HXV08277.1"
                     /translation="MKLCHFEAGLDRPLFLIAGPCVVESRELQMDTAGELKAICQALE
                     IPFIFKSSYDKANRSSGKSFRGLGMERGLEVLAEVRRTIGVPVLTDVHDEQEIPAVAA
                     VVDVLQTPAFLCRQTDFIRAAASAGKPVNIKKGQFLSPWEMKNVVDKAREASGRDNIL
                     VCERGASFGYNNLVSDMRSLAVMRATGCPVVFDATHSVQLPGGQGTASGGQREFVPVL
                     ARAAVASGVAGVFMETHPNPDKALSDGPNAWPLKHMRALLETLKDIDALVKRRGFAEN
                     LL"
BASE COUNT          931 a         2146 c         2032 g          978 t
ORIGIN      
        1 cgtcggggcc accaacggct ggaagatgga gcaggtggtg ccctcgcgcg agctggtgca
       61 gcgcatcgat cgcgagatgc cgatcgagcc ggtgcaaccc aactacgaag gcgaggtggc
      121 ggagcgctgg cgttaccggg atggcagcgg cgaggtgggc ttcatctcct cggtgacgca
      181 ggccttctgc cgcagctgca cccgcgcccg cctgtccgcc gaaggccagc tctacacctg
      241 tctgttcgcc gagcacggca ccgatttccg cggcctgctg cgcggcggcg cttccgacga
      301 cgagatcgcc gatctgatcc acagagtgtg gacggtccgc gccgatcgct attccgagat
      361 ccgcaccgcc gaaaccgcca aggcgcgcaa ggtggagatg tcctacatcg gcggctgatc
      421 cgccccgcgc ttccatggac aagccggcct ctctcgcggc gctcgccgcc gacagcgact
      481 acgatccgaa ctcgatgccg gtggcgaagg cgcgcgacta tatccgcgcc ttcctcaccc
      541 ccatcaccgg cagcgagcgg ctgcacatcc gcgccgctct ggggcgcgtg ctagccgagg
      601 acgtgctctc cggcatcgac gtcccggcgc atgacaactc ggcgatggac ggctatgcgt
      661 tcccgcacgc cgacttgaag agcgtagggg aaacgtcgtt cgcggtcgtc ggcacgtcct
      721 tcgcgggcaa gcccttcgcc ggacaggtgg gtgccggcca ggcggtgcgc atcatgaccg
      781 gcggcgtgat gccggctggc gccgacagcg tggtgatgca ggaacgcgtc acggccagcg
      841 gcgacacggt gcgcatcccg gccggtcagc aggagggcca gaacgtgcgc ctggccgggg
      901 ccgatatccg gcgcggcagc gtggtgttcc ggtgcggaca gatcctgcgg cccgcggaga
      961 tcggcatgct cgcctcgctc ggcatcggcg aggcagcggt attccgcaag ctgcgagtgg
     1021 cgttcttctc caccggcgac gagctggtcg gcatcggcag tccgctcgcc gccgggcaga
     1081 tctacgacag caaccgctac accattcacg gcatgctcac ccgcctgggt tgcgaggtgc
     1141 tcgacctggg cgtgatacgc gacgacccgg ccgcgatcga gcgcgcgttc cgtgaagcgt
     1201 cgcagtccgc cgacgtggtg atcaccagcg gcggggtctc cgtgggcgag gccgatttcg
     1261 tcaagcagat cctcaaccgg ctgggcgagg tgctgttctg gaagatcgcg atgcgcccgg
     1321 ggcgcccgct cgcgtacggt aaattggggc aggcgcattt cttcgggctc cccggcaacc
     1381 cggtgtccgc catggtcacg ttctaccagt tcgtgcgcga cgccctgctg gtgctgatgg
     1441 gcgttcccga accgcagccg cttcccacct tcaaggcgct gtgcacctcg ccgatcaaga
     1501 aggcgcccgg gcgcaccgag ttccagcgcg gcatcctgag ccacgccccg gatgggcgtt
     1561 ggacggtgcg cgtcaccggc gatcagagct cgggcatcct gtcgtccatg agccaggcca
     1621 actgcttcat cgtcctgcgc gacgaccagg gcgccgcgcc ggcaggcacc gaagtcgacg
     1681 tgcaggtgct ggaaggcatc atctgagcgc gccgccggtc accggcgtga tcctcgcggg
     1741 cgggcgcggg cggcgcatgg gccaagtgga caagggctgg gccgagctgg acgggcgtcc
     1801 gctggtggga cgggtgctgg agcgcttcgc gccacaggtc ggccaggtgc tgatcagcgc
     1861 caaccgcaat cgcgagcgct atgcggcgct cggccacgag gtgatcgccg atctcatgcc
     1921 cgatcacgcc gggcctctgg caggactgca tgccgccttc gcccatgcgc gttgcgatct
     1981 gatcgcgacc gtcccctgcg acagcccatg gcttcccctg gatctggtgc aacggctgcg
     2041 taacgccctg gagcgctcga gcgcgcaggt cgcggtcgcc cgctccggcg ggcggctgca
     2101 cccggtgttc ctgctgtgca ggaagacggt ggccggccag ctcgaagcgt atctcgccgg
     2161 cggcgcgcgc aaagccgaag gctggtgtgc aaccctgccc tgtgaacagg tcgacttcga
     2221 cgatcagccc gaggcctttc gcaacatcaa cacaccggcc gacctcgagc gctgagcgcg
     2281 gcgatcacgc ggtcagggcc gaccgggctg ccgttccagc gcctccaggt cttcttcgac
     2341 aaaacgcaga agcgcctcca gcaacgcctc gcgcgggtag gcgttctggg tcgcgaacag
     2401 cgaggcgatc agggggtgag agcggttgac cggtatcgtc aacgccaagt ggtcggcgtt
     2461 gacgtaggcg gcgagcgtgc tgcccgggat cacctggtcg tagaagacga gctggctatc
     2521 gttgcgcgca tcgaccttgc tcaatttatc atagctgccg cgaagcgccg ccgagatgcg
     2581 gtcggggtga gggtacgtca ccaccgagta gtaagggaaa tcctgtggca gcgggttgcc
     2641 cgcgagccat gccctgcgga cgcccggccg caggctgtcg accgccaggc cgtcgccctt
     2701 ctcgcatttt gcgccaggcc agtgtcgcaa caggtcggcc tgggactgtc gcgctcggtt
     2761 ggcgagcggc gagcccccaa cggccccggc ggcgctgacc gccgcggcca ccaccccccg
     2821 gatctccgga tacccgacca ccgcctcgag gaggtccgga gcgcccttcg agtatccgat
     2881 gaggaccagg cgccgctcct catcggggcc aagctgcatt gcgaggaccg tgtcgcggat
     2941 gcgtcgagcg ttgacgctgg tgcccgacag gccgccgact tccagcacct tgaactcgta
     3001 cccgaactgg cgcacatgcg ccgcggcgct gtcgctcgcc tgcagccagt cggcgaagca
     3061 ctcccatccg agccccggca cgatccccgc tatgagtcgc cgccgcgatg gcccgagatc
     3121 gaccggtttg ccagtggccg caggctcggt accgactcgg gtgagcgcct cctcgcaagg
     3181 ccggtagtcc ggaagatcgt gcccatgcgc ctcgagcacc gcgcagaaga tttcacggaa
     3241 gcgcgcgcgc ttgtcgttca cccccgcctg ctgagccggc accagcacca gcggaggtgt
     3301 gtcggcggtg taaggcgaca acggggcggt cgagcaggcc gagatcgaaa tgcaggccgc
     3361 gccggccacc accgcaggaa gccacgcctc gcgaccgatt ggcagcggat cgggccgaga
     3421 caatcaatcc accggcgtgc gtgcagcgct catcttgcgc ttgaagcgca gggcgctatt
     3481 gtgcatcggg cgccgcgcgc tgcggctcga gcacctggat gaacacctcg tcctggcgga
     3541 tcatgccgag ctcgctgcgg gcgcgctcct cgatggcgtc gtagccggtc ttgagatcgc
     3601 gcacttcggc gtccagggcg gcgttgcggg cgcgtagccg ttcgttgctc tgccgttgcg
     3661 cctcgagctg gcggtcgagc tcccacaccc gaacccagcc gcccttgccg aaccacaggg
     3721 gcgtctggat cgccgcgatg aggatcacca ggacgacggc caggacgcgc atcgcaggga
     3781 ctcagaacag ctggtagaac gccccgcgcc cgggatagct ggccgattcg cccaagtcct
     3841 cctcgatgcg cagcagctgg ttgtacttgg ccatgcggtc ggagcgcgac agcgagccgg
     3901 tcttgatctg cagcgtgttc gccgccacgg cgaggtcggc gatgatggtg tcctcggtct
     3961 cgcccgagcg gtgcgacacc accgcggtgt aaccggcgcg cttggccatc tcgatcgcgg
     4021 cgaaggtctc ggtcagcgtg ccgatctggt tgatcttgat cagcaccgag ttggccactc
     4081 cacgccggat gccctcggcc agtatgcgcg tgttggtgac gaacaggtcg tcgccgacca
     4141 gctgcacgct gtcgcccagc cggtcggtga ggatctccca gccgtcccag tcgctctgcg
     4201 ccatgccatc ctcgatgctg aggatcgggt acttgtccac ccacgcgccc agatagtcgg
     4261 cgaactgcgc ggcggtcagt tgcagcccct cggactcgat ccggtagcgg ccttccttga
     4321 agaactccga gctggcgcag tccagcccga tcgccacctg cgtgccggcg ctgtagccgg
     4381 cgccctcgat ggcttgcatg atcaactgca gcgcggcttc gttgccgggc aggttgggcg
     4441 cgaagccgcc ctcgtcgccc accgtggtgg gcatgccctt gccgtcgatc agcttcctga
     4501 gcgcttggaa cacctccgcc ccgcagcgca tggcctccgc gaaactcgca gcgcccaccg
     4561 ggatgatcat gaactcctgc aggtcgaggc tgttgttggc gtgtgccccg ccgttgatca
     4621 cgttcatcat cggcaccggc agctgcatgc gccccgcccc gcccaggtag cggtacagcg
     4681 gcagaccgga gtcctccgcg gccgccttgg ccaccgccag cgacaccgcc agcgtggcgt
     4741 tcgccccgag acgggccttg ttgtcggtgc cgtccagttc gatcagagtc cggtcgatca
     4801 gcgcctgctc gaccgcgtcc agcccgatga tggcctcgca gatctcggta ttgacgttct
     4861 ccaccgcgcg cagcacgccc ttgcccaagt agcgctgcgg atccccgtcg cgcagctcga
     4921 tcgcctcttt ctcgccggtg gatgcgcccg agggcaccgc ggcgcgcccc atcacgcccg
     4981 actcgagcag cacgtccgcc tccaccgtcg ggttgccgcg cgagtcgagg atctcgcgcg
     5041 cgatcacgtc cacgatcgag ctcattgcag gtcccttgtt atgtgctcat gccgcagacg
     5101 cgccggcagg cgcctctcac agcaggttct cggcgaagcc gcgccgcttc accagcgcgt
     5161 cgatgtcctt gagcgtctcc agcagcgcgc gcatgtgctt caacggccag gcgttggggc
     5221 cgtcggacag cgccttgtcc gggttcggat gcgtctccat gaacacgccg gccacgccgc
     5281 tcgccaccgc ggcgcgcgcc agcaccggca cgaactcgcg ctggccgccg ctcgcggtgc
     5341 cctgcccgcc cgggagctgc accgaatggg tagcgtcaaa caccaccggg cagccggtcg
     5401 cgcgcatcac ggcgagcgag cgcatgtccg ataccaggtt gttgtacccg aacgaggcgc
     5461 cgcgctcgca caccaggatg ttgtcccggc cgctggcctc gcgcgccttg tccaccacgt
     5521 tcttcatctc ccagggcgag aggaactgcc ccttcttgat gttcaccggc ttgccggcgg
     5581 aggccgcggc gcggatgaag tcggtctggc ggcacaggaa cgcgggcgtc tgcagcacgt
     5641 ccacgaccgc cgcgaccgcg gggatctcct gctcgtcgtg cacgtcggtc agcaccggca
     5701 cgccgatcgt ccggcgcacc tcggccagga cctccagccc gcgctccatc cccagcccgc
     5761 ggaacgactt gccggagctg cggttggcct tgtcgtagga cgacttgaag atgaacggga
     5821 tctccagcgc ctggcagatc gccttcagct cgcccgccgt atccatctgc agttcgcgcg
     5881 actcgaccac gcagggtccc gcgatcagga acagcggccg gtcgaggccg gcttcgaagt
     5941 ggcagagctt caccgcggcc tcatcgcaag acggcattcg ccacagagga cacagaggaa
     6001 aaacgcaatg ctcagcaagt aaggcagtcc tctgtgccct ctgtgtcctc tgtgactcaa
     6061 cgctgctgca gttgcggtca ccgccct
//
LOCUS       DASPGE010000299         8084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1248905, whole genome shotgun sequence.
ACCESSION   DASPGE010000299 DASPGE010000000
VERSION     DASPGE010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8084
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248905"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VD791_09630"
     CDS             complement(<1..802)
                     /locus_tag="VD791_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXV08278.1"
                     /translation="MNAPPAKPQFAWEDPLLIEEQLTGEERMVRDAARAYAQDKLMPR
                     VLEAHRSEHFHREIMDEMGALGFLGCTIEGYGCAGVNHVCYGLIAREIERVDSSYRSA
                     LSVQSSLVMYPIYAYGSEAQRRKYLPGLARGELVGCFGLTEPGYGSDAGGMITRARKV
                     EGGWLLKGAKMWITNSPIADVFVVWAKDDAGAIRGFILEKGMKGLSAPKIHGKFSLRV
                     SCTGEIVMDEVLVPEQNLLPDVSGLKGPFGCLNKARYGIAWGAMGAAEF"
     gene            983..1426
                     /locus_tag="VD791_09635"
     CDS             983..1426
                     /locus_tag="VD791_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXV08279.1"
                     /translation="MKILMAYDATEEGQAALLSAPELAWLPHDEVRLLAVMPMPSGLF
                     LGEGYVPGEVLEEDRLRAQALLDAAVAGLRSRGFPVEGILTSGEPVDEICSAAKRLGS
                     DLILVRHPRKLSFAARWWKGWVGSSLIEHAPCGVLIAVGPERKSR"
     gene            complement(1435..1671)
                     /locus_tag="VD791_09640"
     CDS             complement(1435..1671)
                     /locus_tag="VD791_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08280.1"
                     /translation="MLAAATAVLAACAPAEPPPPAVNLTGFPPAFRDGYTAGCESARR
                     GITRRDEKRYAQDRQYAAGWRDGRDMCGKPRKPR"
     gene            complement(1850..2098)
                     /locus_tag="VD791_09645"
     CDS             complement(1850..2098)
                     /locus_tag="VD791_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08281.1"
                     /translation="MQRRQGWLFPMMVVAAAAMIAFGCIGIAGITGRLPLAPPGSGPL
                     DSAQAKPSTVFTAERAVQDSSAAAPAHDPGSAHPIVRN"
     gene            complement(2452..3822)
                     /locus_tag="VD791_09650"
     CDS             complement(2452..3822)
                     /locus_tag="VD791_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXV08282.1"
                     /translation="MNTQRARVMLVDDDPDLLRLLSIRLQGAGYGVTAVESGEAALSQ
                     LAVASPDVIITDLRMGGMDGMALFESLQKKNPALPVIILTAHGTIPDAVDATQRGVFG
                     YITKPFDGKALLTQVEKALQVSGSRAMSTGTAGDAGGWRDEIITRSPEMEAILAKARL
                     VASSDASVMIFGESGTGKELLARAIHRASSRATQPFVAVNCGAIPEQLLESELFGHVK
                     GAFTGAASDYKGLFQAAGRGTVFLDEIGDMPLPLQVKLLRVLQEKEVRPIGSTQAVKV
                     DVRIISATHRNLEEAIKAGTFREDLYYRLHVVALSLPSLSQRREDIPVLASHFLKNLA
                     TRYGKTLNGFAQDALEVLVKHNWPGNVRELYNVIEQAVALATTPIVTPALLETAIRGE
                     TGNLSSFESARFDFERDYLAKLLKITNGNVTQAARLAKRNRTEFYKLLQRHHLNPSMF
                     KPGVAA"
     gene            complement(3819..4424)
                     /locus_tag="VD791_09655"
     CDS             complement(3819..4424)
                     /locus_tag="VD791_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08283.1"
                     /translation="MGGPPSWWSALVVGTALVVGTTLGGCMVAAPTQPTTSPPVVVEA
                     AEPGPPPPAPAPPRRVSDSERLLYYYEYVLALTPEQVARESERTQRFFGQHRSEFALM
                     QLVLLRCLPAAAPKDRVQALETLALYLKETRDRPTELRPFALMLNNLLLEQQRQDTEL
                     QAQAQKLKEEAKRYDELKQKLDALVETERKMLERNQETRSQ"
     gene            complement(4424..5839)
                     /locus_tag="VD791_09660"
     CDS             complement(4424..5839)
                     /locus_tag="VD791_09660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXV08284.1"
                     /translation="MLILAGFSLAVVPLIFALVNNAVSIRELATKSQRAVYNAVQATQ
                     SSRQLIEQITSMERSARQFSILAEPQIFSVFEATHRDFLDTVRRMRGLPLMPAQVRML
                     DRMAEIENLLFTSVAGLRDQPRMVADLVDDYVNLSDLARSLDVMGREAVGRETAAMQS
                     LSTDVNSFIYWQLVALIPVALFLVVGAAILILKPIKQIEGALRRIGEGDFQHPVEVSG
                     PRDLEDLGRQIDWVRMRLMELEEQKTRFLHQVSHELKTPLSAIREGAELMADGAVGAM
                     SEPQVEVARILRENTLRLQRLIEDLLNYHTVQFQRSGIHLEKVMLPAVIQRVAEAHRL
                     PMRAKDIRLSVSCPDIALQADQNKLEVILDNLLSNAVKFSPPFGEIMITAHARDSEVV
                     LDVTDEGPGVAPSERERVFDPFFQGKARADAPVKGTGLGLAIVREYVMAHGGKVDIVD
                     SRGQGAHFQVRLPASAPAPGR"
     gene            complement(5918..6706)
                     /locus_tag="VD791_09665"
     CDS             complement(5918..6706)
                     /locus_tag="VD791_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXV08285.1"
                     /translation="MTPDAVREAAQILWNHWQNGHHLSALPLACRPATRAEGYLIQAQ
                     VLAVSGRSLFGWKIAATSPAGQAHIGVDGPLAGRLLTGQVFSDGDSVPLGADRMRVAE
                     PEFAFRMGRDLPPRSTAYRETEVLEAVAALHPAIEIPDSRYEDFPRAGAAQLIADNAC
                     AHQFVLGRPSGGDWRALDLAQHEVVGEVRGRPARTGTGANVLGGPAVALTWLANELSG
                     LGLTLGQGQVVTTGTCLVPLEIAAGDRVLADFGALGRVEARIAD"
     gene            complement(6797..6883)
                     /locus_tag="VD791_09670"
     tRNA            complement(6797..6883)
                     /locus_tag="VD791_09670"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6847..6849),aa:Leu,seq:caa)
     gene            6987..>8084
                     /gene="queA"
                     /locus_tag="VD791_09675"
     CDS             6987..>8084
                     /gene="queA"
                     /locus_tag="VD791_09675"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HXV08286.1"
                     /translation="MRLEDFDFDLPAELIAQHPSERRGESRLLTLDGRSGALSDRRFA
                     DLPELIAASDLLVLNDTRVIKARLLGRKDSGGRVELLVERVLDAHHALALLRASHAPQ
                     PGSAIAVNGIRIRVLEREGEFYRLEFAGPPSVLEILERHGRVPLPPYIERADAPEDER
                     RYQTVYAREPGAVAAPTAGLHFDEAMLGRLRERGVQTAFLTLHVGAGTFQPVRAARIE
                     EHRMHSERYRVPEETVAAVARARGSGGRVVAVGTTSLRALESAARGGRLEPGGGETDV
                     FIVPGFRFQVVDRLLTNFHLPRSTLLMLVAAFAGLDHVLRAYRHAIERRYRFFSYGDV
                     MLIDRTPGLIDRNPGLIDRNPGSIDRSSGSID"
BASE COUNT         1187 a         2778 c         2824 g         1295 t
ORIGIN      
        1 agaactcggc cgcgcccatc gccccccagg cgatgccgta gcgcgccttg ttgaggcagc
       61 cgaagggccc cttcagcccg ctcacatcgg gcagcaggtt ctgctcgggc acgagcacct
      121 cgtccatcac gatctcgccg gtgcaggaca cgcgcaagct gaacttgccg tggatcttgg
      181 gtgccgatag acccttcatg cccttttcca ggatgaagcc gcggatcgcg cccgcgtcgt
      241 ccttggccca gaccacgaac acgtcggcga tgggcgagtt ggtgatccac atcttggcgc
      301 ccttcagcag ccagccgccc tcgaccttgc gcgcgcgcgt gatcatgccg ccggcatccg
      361 agccgtagcc gggctcggtc agcccgaagc agccgaccag ctccccgcgc gccaggccgg
      421 gcaggtactt gcggcgctgc gcttcgctgc cgtaggcgta gatcgggtac atcaccaggc
      481 tggactgcac gctgagggcg gagcggtagc tcgagtccac gcgctcgatc tcgcgcgcga
      541 tcagcccgta gcacacgtgg ttcacgcccg cgcagccgta cccctcgatg gtgcagccga
      601 ggaagccgag cgcgcccatc tcgtccatga tctcgcggtg gaaatgctcg ctgcggtgcg
      661 cctccagcac gcgcggcatg agcttgtcct gggcgtaggc gcgcgccgcg tcgcgcacca
      721 tgcgctcctc gccggtgagc tgctcctcga tgagcagcgg atcttcccag gcgaactggg
      781 gtttggcggg tggggcgttc atgcgctgtc ctggggctgt cctgatcggg ccgggcgctg
      841 cgccgcgagt atacaccggg gtctcggccg gccctccccg gcaccaggta tacacaggcg
      901 ccgggtttac aatgcgatgc cgggcaggga cgccggccat gccgctgatg ccccacgccg
      961 ccgatgccca tggaggctcg cgatgaagat cctgatggcc tacgacgcaa ccgaggaggg
     1021 gcaggcagcg ctgctcagtg cgcccgagtt ggcttggctg ccgcacgacg aggtgcggct
     1081 gctggcggta atgccgatgc cgagcggtct tttcctgggc gagggctacg tgcccggcga
     1141 agtgctggag gaagaccgcc tgcgggcgca ggcgctgctc gatgccgctg tggcggggct
     1201 gcgcagccgc ggctttccgg tggagggcat tctcacctcg ggcgagccgg tggacgagat
     1261 ctgcagcgcc gccaagcggc tgggcagcga tctgatcctg gtgcgccacc cgcgcaagct
     1321 gtctttcgcc gcccgctggt ggaagggctg ggtgggctca tcgctgatcg agcatgcgcc
     1381 ctgcggcgtg ctgatcgcgg tgggcccgga gcgcaagtcg cggtaaccga agcgtcagcg
     1441 cggcttgcgc ggcttgccgc acatgtcccg gccgtcgcgc cagccggcgg cgtactggcg
     1501 gtcctgggcg tagcgcttct cgtcgcgccg cgtgatcccg cggcgcgcgc tttcgcagcc
     1561 cgcggtatag ccgtcccgga acgccggcgg gaagccggtg agattgaccg cgggcggggg
     1621 cggctcggca ggcgcgcagg cggccaggac ggcggtcgcc gccgccagca tccacggcgc
     1681 cgcacgccgc cgacagcggg cgcctgtgga ggtcttcaca gctttttcag gggcttagaa
     1741 aaaaagatgc ccgacaaggg aggcaatcgg gcagaaaaga gagcgctgac gcgcacgaaa
     1801 tcgatcggtt ggcggtcggg tcggctggga ctccctccgg cggcagacgc tagtttcgca
     1861 cgatcgggtg tgcggagccg ggatcgtgcg caggggcggc tgcgctcgag tcttgcaccg
     1921 cacgctccgc ggtgaacacg gtggacggct tggcctgggc gctgtcgagg ggcccgctcc
     1981 cgggcggcgc aagcgggagg cggccggtga tgcccgcgat gccgatgcag ccgaaggcga
     2041 tcatggcggc cgcggcgacc accatcatcg gaaacagcca tccttgccgg cgttgcatca
     2101 catcctacct cgcgtgtgcg gcgccacatg cggcgcacgg tctgcgaccg cggcgcctgc
     2161 agcatggacg acaaggcgca tcgcggtcct tcctcgcacg aagcccaatg caactcgtgt
     2221 accaattgct aagtgcttga acgcggtatc aatgttcgcc gccgccgcga ggcgttctgt
     2281 caccggtcaa cgacgcggcc gcgagcgccc gagcaagatg ctgatatcgc ggatatttaa
     2341 atgtcgttga cgaatgacaa cggcgtattg cgcgcggccc gcacggcgac atgcgccggg
     2401 ccgcagcgcc gccgccgctt gcgcttgcgt cgtcgcgcac accgcgcgct gtcaggccgc
     2461 cacgcccggc ttgaacatcg aggggttgag gtggtggcgt tgcagcagct tgtagaactc
     2521 ggtgcggttg cgcttggcca ggcgcgcggc ctgcgtcacg ttgccgttgg tgatcttgag
     2581 cagcttggcg aggtagtcgc gctcgaaatc gaagcgcgcc gattcgaacg aggacaggtt
     2641 gccggtctcc ccgcggatcg cggtttccag cagcgccggc gtgacgatcg gcgtggtggc
     2701 gagcgccacc gcctgctcga tcacgttgta cagctcgcgc acgttgcccg gccagttgtg
     2761 cttgaccagc acttccagcg cgtcctgggc gaagccgttg agcgtcttgc cgtagcgcgt
     2821 cgccaggttc ttgaggaagt gcgacgccag caccgggatg tcctcgcggc gctgcgacag
     2881 cgacggcagc gacagcgcca ccacgtgcag ccggtagtac agatcctcgc ggaaggtgcc
     2941 ggccttgatc gcctcctcga gattgcggtg cgtggccgag atgatgcgca cgtccacctt
     3001 caccgcctgc gtggagccga tcggccgcac ctccttttcc tgcagcacgc gcagcagctt
     3061 cacctgcagc ggcagcggca tgtcgccgat ctcgtccagg aacacggtgc cgcggccggc
     3121 ggcctggaac agccccttgt agtcggatgc cgcgccggtg aaggcgccct tgacgtgccc
     3181 gaacagctcg gattcgagca gctgctcggg gatcgcgccg cagttgaccg cgacgaaggg
     3241 ttgcgtggcg cgcgagctgg cgcgatggat ggcgcgcgcc agcagctcct tgccggtgcc
     3301 gctctcgccg aagatcatca cgctggcgtc gctgctcgcc accagccgcg ccttggccag
     3361 gatcgcttcc atttcggggc tgcgcgtgat gatctcgtcg cgccagccgc cggcgtcgcc
     3421 ggcggtgccg gtgctcatgg cgcggctgcc gctcacctgc agagccttct cgacctgggt
     3481 gagcagggcc ttgccgtcga agggcttggt gatgtagccg aacacgccac gctgggtggc
     3541 gtccaccgcg tcggggatgg tgccgtgcgc ggtgaggatg atcaccggca gcgccgggtt
     3601 cttcttctgc agcgactcga acagcgccat cccgtccatg ccgcccatgc gcaggtcggt
     3661 gatgatcaca tcggggctgg ccaccgccag ttgcgacagc gccgcctcgc cgctctcgac
     3721 cgccgtgacg ccgtagccgg cgccctgcag gcggatcgac agcaggcgca gcagatcggg
     3781 atcgtcgtct accagcatca cacgggctcg ttgcgtattc attggctcct agtctcctgg
     3841 ttgcgctcga gcatcttccg ctcggtttcg accagcgcgt cgagcttctg cttgagctcg
     3901 tcgtagcgct tcgcttcctc cttgagcttc tgtgcctggg cctgcagctc cgtgtcctgc
     3961 cgctgctgct ccaggagcag attgttgagc atcagcgcga agggccgcag ctccgttggc
     4021 cgatcgcggg tttccttcag atacagggcc agcgtttcca gcgcctgcac ccggtccttc
     4081 ggcgccgccg ccggcaggca tctgagcagc accagctgca tcagcgcgaa ctccgaccgg
     4141 tgctggccga agaagcgctg cgtgcgctcc gactcccggg ccacctgctc cggggtgagc
     4201 gccaagacgt actcgtaata atacaggaga cgctccgagt cgctcacccg gcgcgggggt
     4261 gcgggcgcgg gcggcggcgg ccccggctcg gcggcctcga ccaccaccgg aggcgaggtg
     4321 gtgggttgcg tcggcgccgc gaccatgcac ccgcccagtg ttgtaccgac gaccagtgcc
     4381 gtgccgacga ccagcgccga ccaccaactc ggcggccctc ccatcagcgc cccggtgcgg
     4441 gggccgacgc cggcaaccgc acttgaaaat gcgcaccctg accgcggctg tccacgatgt
     4501 ccaccttgcc gccatgcgcc atcacgtact cgcgcacgat cgccaggccg aggccggtgc
     4561 ccttgaccgg cgcatccgcg cgcgccttgc cctggaagaa ggggtcgaac acgcgctcgc
     4621 gctcggacgg ggccacaccg ggcccctcgt cggtcacgtc cagcaccact tcgctgtcgc
     4681 gggcatgcgc ggtgatcatg atctctccga acggtggcga gaacttcaca gcattggaca
     4741 gaaggttgtc caggatcact tccagcttgt tctgatcggc ctgcagcgcg atgtccgggc
     4801 agctcacgct gaggcggatg tccttcgcgc gcatcggcag cctgtgcgcc tcggcgaccc
     4861 tctggatcac cgccggcagc atcactttct ccaggtggat gccggagcgc tggaactgca
     4921 ccgtatgata gtttagcaag tcttcgatca ggcgctgcag ccgcagggtg ttctcgcgca
     4981 ggatgcgcgc cacctccacc tgcggctccg acatcgcccc caccgcgcca tcggccatca
     5041 gctcggcgcc ctcgcggatc gccgacagcg gcgtcttcag ctcgtgcgag acctggtgca
     5101 ggaagcgcgt tttctgctcc tccagctcca tcagccgcat gcgcacccag tcgatctggc
     5161 ggcccaggtc ctccaggtcg cgcgggccgg atacctccac cgggtgctgg aaatcgccct
     5221 cgccgatgcg cctgagcgcc ccctcgatct gcttgatcgg cttgaggatc aggatcgccg
     5281 cgccgaccac caggaacagc gccaccggga tcagggccac cagctgccag tagatgaagc
     5341 tgttgacgtc ggtggacagc gactgcatcg ccgcggtctc gcgccccacc gcctcgcgtc
     5401 ccatgacgtc cagcgagcgc gccagatcgc tcaaattgac gtagtcgtcc accaggtcgg
     5461 ccaccatgcg cggctggtcg cgcagcccgg ccacgctggt gaacagcagg ttctcgatct
     5521 ccgccatgcg atcgagcatg cgcacctggg ccggcatcag cggcagcccg cgcatgcgtc
     5581 gcaccgtgtc gaggaaatcg cggtgcgtgg cttcgaacac cgagaagatc tgcggctcgg
     5641 cgaggatgga gaactggcgc gcgctgcgct ccatgctggt gatctgctcg atcagctgcc
     5701 ggctgctctg ggtggcctgc accgcgttgt acaccgcgcg ctggctcttg gtggccagct
     5761 cccggatcga caccgcgttg ttgaccagcg cgaagatgag cggcaccacg gcgagcgaga
     5821 agcccgccag gatcagcatc ggaaacgact tgggatagaa gcgccacata cgggccgcgc
     5881 tggctgattc ctaggttcga tgatgtagca aatgcgctca gtcggcgatg cgcgcctcga
     5941 cgcgccccag cgcgccgaaa tcggcgagga cgcgatcgcc ggcggcgatc tcgagcggga
     6001 ccaggcaagt cccggtggtc acgacctggc cctgcccgag ggtgaggccc aggccggaga
     6061 gctcgttcgc cagccaggtg agcgccacgg cggggccgcc gagcacgttg gcgcccgtcc
     6121 cggtgcgcgc cgggcggccg cgcacctcgc ccacgacctc gtgctgcgcg aggtccagcg
     6181 cacgccagtc gccgccgctc gggcggccca ggacgaactg gtgggcgcag gcgttgtccg
     6241 cgatgagctg cgcggcgccc gcacgcggga agtcctcgta gcgcgagtcc ggaatctcga
     6301 tcgccgggtg cagcgcggcc accgcttcga gcacctcggt ctcgcgatag gcggtgctgc
     6361 gcgggggcag atcgcggccc atgcggaagg cgaactcggg ctccgccacg cgcatccggt
     6421 cggcgccgag cggcacactg tcgccatccg agaacacctg cccggtcagc aggcgccccg
     6481 cgagcgggcc gtccaccccg atatgcgcct gcccggcggg gctggtggcg gcgatcttcc
     6541 agccgaacag cgagcgcccg gagacggcca gcacctgcgc ctggatgagg tagccctcgg
     6601 cgcgggtggc cgggcggcag gccagcggca gggcggacag gtggtgtccg ttctgccagt
     6661 ggttccacag gatctgcgcg gcttcgcgca cggcatcggg tgtcatggtt gttgctcggt
     6721 caccgacagc ggaagtgtag gcgacaattt cagcatcggc gcgcaccggc ccggctgaag
     6781 gcggcggtgg gataactggt gccgggagcg agaatcgaac tcgcacgccc gtgaaggcgc
     6841 gggattttga gtcccgtgcg tctaccagtt ccgccatccc ggcacggcgt agggaggcgg
     6901 attatccggg atttgaccgg cggcggcaac gcggccgcgc gcgcgggcgg gcttcgagcc
     6961 gggcggggcg gctataatcg cgcgccatgc ggctcgagga tttcgacttc gacctgcccg
     7021 ccgagctgat cgcgcagcac ccgagcgagc gccgcggcga gagccggttg ctcaccctcg
     7081 acggtcgcag cggcgcactg tccgatcgcc gcttcgccga tcttcccgag ctgattgccg
     7141 cctccgacct gctcgtgctc aacgacacgc gcgtgatcaa ggcgcgcctg ctggggcgca
     7201 aggacagcgg cgggcgcgtc gagctgctgg tggagcgggt gctggacgcg caccacgcgc
     7261 tggcgctgct gcgcgccagc cacgcgccgc agcccggcag cgcgatcgcc gtgaacggca
     7321 ttcggatccg cgtgctggag cgcgagggcg aattttatcg gctcgagttc gcgggcccgc
     7381 cctcggtgct ggagattctc gagcggcacg gcagagtgcc gctgccgccc tatatcgagc
     7441 gcgcggacgc tccggaggac gagcgccgct atcagaccgt ctacgcacgc gagcccggcg
     7501 ccgtggcggc ccctacggcc ggtctgcatt tcgacgaggc gatgctcggc cggctgcgcg
     7561 agcgcggcgt gcagacggcg ttcctgacgc tgcacgtggg cgcgggcacg ttccagccgg
     7621 tgcgcgccgc gcgcatcgag gagcaccgca tgcacagcga gcgctaccgg gtgcccgaag
     7681 agacggtggc cgcggtggcg cgggcgcgcg ggtcgggcgg gcgcgtcgtg gcggtgggga
     7741 ccaccagcct gcgcgcgctc gaatcggcgg cgcgcggcgg ccgcctcgag cccgggggcg
     7801 gcgagaccga tgtgttcatc gtgccggggt tccgcttcca ggtggtggac cgcctgctca
     7861 ccaatttcca tctgccgcgc tccacgctgc tgatgctggt ggccgcattc gcgggcctgg
     7921 accacgtgct gcgcgcctat cgtcacgcga tcgagcggcg ctaccgattc ttcagctatg
     7981 gcgacgtcat gttaattgat cgaacccccg ggttgattga tcgaaatccc gggttaattg
     8041 atcgaaaccc cgggtcgata gatcgaagct ccgggtcaat cgac
//
LOCUS       DASPGE010000300         3393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1132278, whole genome shotgun sequence.
ACCESSION   DASPGE010000300 DASPGE010000000
VERSION     DASPGE010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132278"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VD791_09680"
     CDS             complement(<1..1006)
                     /locus_tag="VD791_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXV08287.1"
                     /translation="MLKKLATAAAAAIALLGVQHAPAGRLDPALETRLSAAGPSEKIR
                     IIVEMKEQARPRDLIRALPKGARKAKLKALVAGLKDKARRRQAGIEVELAREQTLGAA
                     ERVVPFWVFNGLALRANPGLIRRLAARDDVLEVRLDQEIPPPPRPAEASAPSGPTEWN
                     IAHIRAPEVWALDPAYNGQGTVVGSFDTGVDLAHPELFERYRGNHAISWFDPYGEHSL
                     PTDFNGHGTHTIATAVGGGAGGSAIGVAPGAKWIAAKAWADNGVGLASAFHSIFEWFL
                     APGGDPDNAPDVVNNSWAFIAAGCNSEFVPDIEAWRAAEIFPAFAAGNAGPSAGSVRS
                     P"
     gene            1238..1765
                     /locus_tag="VD791_09685"
     CDS             1238..1765
                     /locus_tag="VD791_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08288.1"
                     /translation="MIQSIAWQVTVVLVVLFALGFLFVARRASERSDYEPIKSKASRT
                     RGIVFWALVLVSIAVIAVSLGHLPYVARAGGSDAVEVIRASGHQWRWELSKTEVVAGK
                     PVEFHVTGADVNHGFGIYDPQLRLVAQTQAMPGYTNVLRHTFAQPGTYRILCLEYCGL
                     AHHAMMAELTVLASE"
     gene            1776..3227
                     /locus_tag="VD791_09690"
     CDS             1776..3227
                     /locus_tag="VD791_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXV08289.1"
                     /translation="MSAASPDTLTLGARRAVLAYVAVTIVVVALMMLLGLLLRLSQAQ
                     ILVLPPDLFYQVMTAHGIGMVGIAGLGGAAIMWYFLSQYVKLNTSILAANLVLFLVGV
                     VFILGAIFLGGFAAAWTFLFPLPAQPLGVWSIGAAAAYLIGVLLVGVGFLLFYLDTAW
                     AILVKYGSLGRALGWPQLIGRDDGAAPPPTVVASTVVSVINILSIVVGAVILTVCLVN
                     LYVPEFAVDALLAKNLIFFFGHVFINAAIYMTVIAVYEILPRYAGRPWPSTRVFLAAW
                     TATMIMVLIVYPHHLLMDFAMPVWALVMGQVISYASGLPVLAVTMIGTLAIVYRSGLR
                     WDLVSGLLFLSVFGWAAGVLPAIIDATIVVNNVMHNTLWVPGHFHFYLVLGLVAMLVA
                     FAYHLAKSRGGLQGHGLDALSFWTFAAAGLGLAGSFLLSGRHSIPRRWAVHLPEWVPY
                     DRIGSVFAALVVIAVLVFTVRFLTRLGAMARPA"
BASE COUNT          446 a         1138 c         1182 g          627 t
ORIGIN      
        1 cggggctgcg cacgctgccg gcgctcggtc cggcgttgcc ggcggcgaac gccggaaaga
       61 tctcggcggc gcgccaggcc tcgatgtcgg gtacgaactc gctgttgcag ccggccgcga
      121 tgaaggccca ggagttgttc accacgtcgg gcgcgttgtc cggatcgccg ccgggggcga
      181 ggaaccactc gaagatgctg tggaaagcgg aggccaggcc gacgccgttg tccgcccagg
      241 ctttggccgc gatccacttc gcgcccggcg ccacgccgat ggcgctgccc ccggcgccgc
      301 cgcccaccgc ggtggcgatg gtgtgggtgc cgtgcccgtt gaaatcggtc ggcagagaat
      361 gctcgccgta cggatcgaac cagctgatcg catggttgcc gcggtagcgc tcgaacaact
      421 cgggatgagc gaggtcgacg ccggtgtcga agctgccgac gacggtcccc tgaccgttgt
      481 atgcgggatc gagcgcccac acctcgggcg cgcggatgtg cgcgatgttc cattcggtcg
      541 ggcccgaagg cgcgctggcc tccgccggac gcggcggcgg cgggatctcc tggtcgaggc
      601 gcacttccag gacgtcatcg cgcgccgcca gcctgcggat cagcccgggg ttggcacgca
      661 gcgcgagacc gttgaacacc cagaacggca ccacgcgctc ggcagcaccc agcgtctgct
      721 cgcgcgccag ttcgacttcg ataccggcct ggcgccgcct ggccttgtcc ttgaggcccg
      781 ccaccagcgc cttgagcttg gccttgcgcg cgcccttggg cagcgcccgg atcaggtccc
      841 ggggccgggc ctgctccttc atctcgacga tgatgcggat cttttcgctc gggccggcgg
      901 cgctcaaccg ggtttcgagc gcaggatcga gacggccggc cggtgcgtgc tggacgccca
      961 gcagggcgat ggcggccgca gccgccgtgg cgagcttctt gagcacggtg tcccctcccc
     1021 gtgtgtgacc cccgcccgtg cctgggggcc atcgcccgcc gagcgatccc gcgggcatac
     1081 cacaaaagca tagtacatct gggtggcggc aggggagcca ttggcggcgc ttcgtcaatg
     1141 gattgtcaaa actcgcctcg caatgcgcta caatccgtca ctcggtggac ccatcccagg
     1201 tggacgtggg aagcgtcaac ttcaggcgga ggcgagcatg attcaaagca tcgcttggca
     1261 ggtcaccgtc gttctcgtcg tcctgttcgc gctaggcttc ctcttcgtgg cgcggcgcgc
     1321 ctccgagcgc agcgactacg agcccatcaa gagcaaggcc tcgcgcaccc gcggcatcgt
     1381 gttctgggcg ctggtgctgg tgtccatcgc ggtcatcgcc gtttcgctgg ggcatctgcc
     1441 ctacgtcgcg cgcgccggcg gatcggatgc cgtggaggtg atccgcgcct ccgggcacca
     1501 gtggcgttgg gagctgagca agaccgaagt ggtggccggc aagccggtgg agtttcacgt
     1561 caccggcgcg gacgtgaacc acggcttcgg catctacgac ccgcagctgc ggctggtcgc
     1621 ccagacgcag gcgatgcccg gctataccaa cgtgctgcgg catacgttcg cgcaacccgg
     1681 cacgtaccgg atcctgtgcc tcgagtactg cggcctcgcg caccacgcga tgatggccga
     1741 gttgaccgtg ctcgcatccg agtagggagg tgaccatgtc tgccgcgtcc cccgacacgc
     1801 tcaccctggg tgcccggcgc gccgtgctgg cgtacgtggc cgtcaccatc gtggtggtcg
     1861 cgctgatgat gctgctgggg ctgctgctgc gcctgtccca agcgcagatc ctggtactgc
     1921 ctcccgatct gttctaccag gtcatgaccg cccacggcat cggcatggtc ggcatcgccg
     1981 ggctgggcgg ggccgcgatc atgtggtatt tcctgagcca gtacgtcaag ctcaatacct
     2041 cgatactggc cgccaacctg gtgctgttcc tggtcggcgt ggtgttcatc ctcggcgcga
     2101 tcttcctggg cgggttcgcc gccgcctgga ccttcctgtt cccgctgccg gcgcagccgc
     2161 tgggggtatg gagcatcggc gccgccgctg cctatctgat cggcgtgctg ctggtcggcg
     2221 tcggctttct gctgttctac ctcgacacgg catgggcgat cctggtcaag tacggcagcc
     2281 tggggcgcgc cctgggctgg ccgcagctca tcggccgcga cgacggagcg gcgccgccgc
     2341 cgaccgtggt ggcgagcacc gtagtcagcg tgatcaacat cctgtccatc gtcgtgggcg
     2401 cggtgatcct caccgtttgt ctggtgaacc tgtacgttcc ggaattcgcg gtcgatgcgc
     2461 tgctcgccaa gaacctgatc ttcttcttcg gccacgtgtt catcaacgcc gccatctaca
     2521 tgacggtgat cgccgtgtac gagatcctgc cgcgttacgc gggccggccg tggccctcga
     2581 cacgcgtgtt cctggcagcg tggacggcga ccatgatcat ggtcctgatc gtgtacccgc
     2641 accatctgct gatggacttc gcgatgccgg tgtgggccct ggtcatgggc caggtcattt
     2701 catatgccag cggcctgccg gtgctcgccg tgaccatgat cgggacgctg gcgatcgtgt
     2761 atcgctccgg gctgcgctgg gacctggtgt ccggactgct gttcctgtcg gtgttcggct
     2821 gggccgcggg cgtgctgccc gcgatcatcg acgccaccat cgtggtcaac aacgtgatgc
     2881 acaacacgct gtgggtgccg gggcacttcc acttctacct ggtcctcggc ctggtggcga
     2941 tgctggtcgc gttcgcttat cacctcgcca agtcccgcgg cgggctgcag ggccacggtc
     3001 tggatgcgtt gagcttctgg accttcgccg ccgccgggct ggggctggcc gggagtttcc
     3061 tgctctcggg tcgacacagc attccgcggc gctgggcggt gcacctgccc gagtgggtgc
     3121 cctacgaccg catcggcagc gtgttcgcgg cgctcgtggt gatcgcagtg ctggtcttca
     3181 cggtgcgctt cctgacgcgg ttgggggcga tggcgcggcc ggcatgacgt tgcgcagcgc
     3241 cctgtgcgcg ctcctggtgt tgtgcctggg gagcgcaggg ctatgggcgg gcacggacgg
     3301 actgcgggcg ttcacctcgg agcaggcgcg gcgcttggcc gtgcaacgca cgccgcgccc
     3361 ggtgccggac gtcgtgctgc aggaccagga cgg
//
LOCUS       DASPGE010000301         2655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_664129, whole genome shotgun sequence.
ACCESSION   DASPGE010000301 DASPGE010000000
VERSION     DASPGE010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664129"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VD791_09695"
     CDS             <1..733
                     /locus_tag="VD791_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HXV08290.1"
                     /translation="RRVELEVLRYNPDTDREPHFQRYAVDCQEEWVVLDALNHVKETL
                     DPTLSYRWSCHMAVCGSCGMMVNGEPKLACKAFLRDYAGVIRVEPLRHFPVERDLVTV
                     IDDFVAKLARVKPYLIPKDQAPEAGEYRQTPAQLKRYKQFTLCINCMLCYAACPEYGL
                     IPKYLGPAAIALGHRWNRDSRDGGREARQEELAATEGAWECSFVGACSEVCPEHVDPA
                     GAIQQVKIESTVDWYREHLMPWTKK"
     gene            730..1119
                     /locus_tag="VD791_09700"
     CDS             730..1119
                     /locus_tag="VD791_09700"
                     /inference="COORDINATES: protein motif:HMM:NF014366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08291.1"
                     /translation="MSRRPFARPITSEWIFRHPRYLRYMVREFSCLFIGGWTLLMVWG
                     LKALSGGPAAWGAFLELLATPASIVFHLLTLAFALYHSVTWFNLTPKALPVQRGEEFL
                     PDWVVAGAHFAAWAVVSAGLLYFAGAF"
     gene            1122..1463
                     /gene="frdD"
                     /locus_tag="VD791_09705"
     CDS             1122..1463
                     /gene="frdD"
                     /locus_tag="VD791_09705"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF003977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HXV08292.1"
                     /translation="MARSNKPVVWGPFAAGGTLTAFLTPALVVVTLLAALGHAPDLLA
                     YDRLQALAGHWVAKVAIAGVVFLSLWSAAHRLRITCYDLGIRADTLVATIVYAVALAG
                     AGASVLYLLRI"
     gene            1479..2237
                     /locus_tag="VD791_09710"
     CDS             1479..2237
                     /locus_tag="VD791_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV08293.1"
                     /translation="MNPPPVATREIAREERFNNVWTQELNDVFADVAPYYDRANYIAS
                     LGLWGWFLRKFMATVDIKPQERVLDVCAGTNAISIALLKREPTLDVHAIDRSAAMQEV
                     GRQRAEALGFRIKSVIDDVHTLPYPDNTFDVVTLQFASRHLRIRRVAEEIRRVLKPGG
                     RFYHSDMLRPGNPIVEKLYYAYLRFCLWFTGFLFRSGPAALNCKKYFIQALQMFYSAR
                     ELSDMLEDVGFRDVTATTVFSGMLGFHRAVKPAA"
     gene            2315..>2655
                     /locus_tag="VD791_09715"
     CDS             2315..>2655
                     /locus_tag="VD791_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08294.1"
                     /translation="MPLALRAALPALEPFLAENVAKEIRKDALVIRRAATAPSPGAAR
                     EVLAAAREIDREFLGRIGGLPLRIRIPYDRIEPLRLRRIELGLDTAGRILDAWRQRRR
                     LRQAFAPGELE"
BASE COUNT          386 a          928 c          906 g          435 t
ORIGIN      
        1 gaggcgcgtc gagctcgaag tgctgcgcta caacccggac acggaccgcg agccgcattt
       61 ccagcgctac gccgtcgact gccaggaaga gtgggtggtg ctcgacgcgc tcaaccacgt
      121 caaggaaacc ctcgatccga cgttgagtta ccgctggtcc tgccacatgg cggtgtgcgg
      181 cagctgcggc atgatggtca acggcgagcc caagctcgcc tgcaaggcgt ttttgcgcga
      241 ctacgccggg gtgatccgcg tggagccgct gcgccacttc ccggtcgagc gggacctggt
      301 gaccgtgatc gacgacttcg tcgccaagct ggcgcgcgtc aagccctacc tcattccgaa
      361 ggaccaggcg ccggaagcgg gcgagtaccg ccagacgccg gcgcagttga agcgctacaa
      421 gcagttcacg ctgtgcatca actgcatgct gtgctacgcg gcctgcccgg aatacggcct
      481 gattcccaag tacctcggcc cggcggcgat cgcgctcggg caccgctgga accgggactc
      541 gcgcgacggc gggcgcgagg cgcgccagga ggagctcgca gccaccgaag gtgcgtggga
      601 gtgctccttc gtgggcgcct gctcggaggt ctgtccggag cacgtagacc cggcgggcgc
      661 catccagcag gtcaagatcg agagcaccgt cgactggtac cgggagcacc tgatgccctg
      721 gacgaagaaa tgagccgccg gcctttcgcg cgcccgatca cctcggagtg gatcttccgc
      781 cacccgcgct acctgcgcta catggtgcgc gagttctcgt gcctgttcat cggcggctgg
      841 acgctgctga tggtgtgggg gctgaaagcg ttgtccgggg gcccggccgc ctggggcgcg
      901 ttcctcgagc tgctggcgac tccggcgagc atcgtcttcc acttgttgac gctcgccttc
      961 gcgctatatc acagcgtcac ctggttcaac ctcacgccca aggcgctgcc cgtgcagcgg
     1021 ggcgaggagt tcctgccgga ctgggttgtc gccggcgcgc atttcgccgc ctgggcggtg
     1081 gtgtccgcgg ggctgctcta cttcgcggga gcgttctagc catggcgcgc tccaacaagc
     1141 cggtcgtctg ggggccgttc gccgcgggcg gcacgctcac ggccttcctc acgcccgcgc
     1201 tggtcgtcgt cacgctgctc gcggcgctcg gccacgcgcc cgatctgctg gcctacgacc
     1261 ggctgcaggc gctggcgggc cattgggttg ccaaggtcgc gatcgctggg gtggtgttcc
     1321 tttcgctctg gagcgctgcg caccgcctgc gcatcacctg ctatgacctt ggcatccgcg
     1381 ccgataccct ggtggcgacg atcgtgtacg ccgtggccct tgccggggcg ggggcgtcgg
     1441 tgctttactt gctgcggatc tgacggaggg aggccccgat gaatccaccg cctgtcgcca
     1501 cccgcgaaat cgcccgggaa gagcgcttca acaacgtatg gacgcaggag ctgaacgacg
     1561 ttttcgccga cgtcgcgccg tactacgacc gggcgaacta catcgccagc ctcgggctgt
     1621 ggggctggtt cctgcgcaag ttcatggcca cggtggacat caagccgcag gagcgcgtgc
     1681 tggacgtctg cgccggcacc aacgccatca gcatcgcgct cctgaagcgc gagccgacgc
     1741 tggacgtgca cgccatcgac cgcagcgcgg cgatgcagga ggtcggcagg cagcgcgccg
     1801 aggcgctcgg ctttcgcatc aagagcgtga tcgacgacgt gcacacgctg ccctacccgg
     1861 acaacacctt cgacgtcgtc accctgcagt tcgcctcgcg ccacctgcgc atccggcggg
     1921 tcgccgagga gatccggcgc gtgctcaagc ccggcgggcg cttctaccac agcgacatgc
     1981 tgcggccggg caacccgatc gtggagaagc tgtactacgc ctacctgcgc ttctgcctgt
     2041 ggttcacggg cttcctgttc cgcagtggcc cggcggcgct caactgcaag aaatacttca
     2101 tccaggcgct gcagatgttc tactccgcgc gcgagctgag cgacatgctg gaggacgtcg
     2161 gctttcgcga cgtcacggcc acgacggtgt tcagcggcat gctcggcttt caccgggccg
     2221 tcaagccggc cgcctgaacc gcgagcgcgc actcgccgcg ctgcacggcc ggctgctgcg
     2281 ggagttcagc cggcgcacca ccgacgcgct gcgcttgccg ctggcgctgc gcgccgcgct
     2341 gccggccctc gagcccttcc tcgccgagaa cgtggccaag gaaatccgca aggacgcgct
     2401 ggtgatccgc cgcgcggcca cggccccgtc gcccggcgcg gcgcgcgaag tgctcgccgc
     2461 cgcgcgcgag atcgaccgcg agttcctggg gcgcatcggc ggcttgccgc tgcgcatccg
     2521 gattccgtac gacaggatcg agcctctgcg gctgcggcgc atcgagctcg ggctggatac
     2581 cgccggccgc atcctcgacg cttggcggca gcgccgccgc ctgcgccagg ccttcgcgcc
     2641 gggcgagctc gagca
//
LOCUS       DASPGE010000302         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_430475, whole genome shotgun sequence.
ACCESSION   DASPGE010000302 DASPGE010000000
VERSION     DASPGE010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430475"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="VD791_09720"
     CDS             complement(<1..161)
                     /locus_tag="VD791_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaD"
                     /protein_id="HXV08295.1"
                     /translation="MLAEDAASRGLGMRLDAIAPGRACMSMTVRADMLNGFKVCHGGF
                     IATLADSAFA"
     gene            complement(241..1050)
                     /gene="paaG"
                     /locus_tag="VD791_09725"
     CDS             complement(241..1050)
                     /gene="paaG"
                     /locus_tag="VD791_09725"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HXV08296.1"
                     /translation="MASAFRSVALEVSEGVATLTLNRPERLNAFNDTMHAEIREVLAR
                     IGSHRTVRAMILTGAGRAFCAGQDLADRSVAPGDEQRDAPRDLGASLEANYNPFVLAL
                     RALPLPVLCAVNGVAAGAGASIALACDVVVAARSASFIQAFSNLGLVPDCGGTYFLAR
                     TLGAPRALGLALFGERLSAEQAHAWGLIWQCVDDAQLMPTVRTLAAKLASGPTRGFAL
                     TKQAIYAAEQRALAAQLDLERDFQRELGRTFDYREGVAAFLEKRAPQFKGE"
     gene            1278..1685
                     /locus_tag="VD791_09730"
     CDS             1278..1685
                     /locus_tag="VD791_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08297.1"
                     /translation="MMLSEMMQEALEQGIRQVRAAGSRLHPFLVVEPAKILFLFDPSG
                     QSEPMEMALCAIREHAPACERVVLVLNTTLTARDGRKSDAVVAMGSERGQETGPMWAQ
                     IYRPGSWFRRFRVEGERETIGEVKNLIREALAA"
     gene            complement(1713..>2752)
                     /locus_tag="VD791_09735"
     CDS             complement(1713..>2752)
                     /locus_tag="VD791_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXV08298.1"
                     /translation="SGYNEATFGAQPIGVRNPYDRTRFAGGSSSGTAAAIAARIAPGG
                     LGSDTGGSSRIPSAVTGGAGFRPTIDRYDNAGVAPISHTRDTVGPMARTVADVAVLDA
                     VIAGGKVPAPASLKGVKLGVYRAYFFNNLDADTGSVMNAALDKLAKAGAEIVEVDMPE
                     LQKLNDGASFPVALYEAYDDMKAYLDKYQTGKTVEELAAMIASQDVKGTYSALVVPRK
                     LPTPDGLIDAAPVYEAAIKQARPALIKHYADTFAKYGIEALIFPTTPHVAAVQGPDAS
                     KLETMLLFIQNTDPGSNAGVPGLSIPAGLGPTTGLPVGIEIDGPRGSDTRLLAIGMAI
                     EKVLGPTPAPK"
BASE COUNT          415 a          923 c          969 g          445 t
ORIGIN      
        1 gcgaaggccg aatccgccag cgtggcgatg aagccgccgt ggcagacctt gaaaccgttg
       61 agcatgtcgg cgcgcaccgt catcgacatg caggcgcggc ccggcgcgat cgcgtccagc
      121 cgcatgccga gcccgcgcga agccgcgtcc tcggccagca tgccgtcgcc gacccgctcg
      181 gcgatggcct gtgggctcaa cgccgtctgg ttcactgagc tcacctcccg tccaccggag
      241 tcattctcct ttgaattgcg gcgcacgctt ctctaggaat gcggccacgc cttcgcggta
      301 atcgaacgtg cggcccagct cgcgctggaa atcgcgttcc aggtcgagct gggcggcgag
      361 cgcgcgctgc tcggcggcgt agatcgcctg cttggtgagg gcgaaaccgc gcgtggggcc
      421 gctggcgagt tttgctgcga gcgtgcgcac cgtcggcatg agctgcgcgt cgtccacgca
      481 ttgccagatc aggccccagg cgtgcgcttg ctccgcgctc aggcgctcgc cgaacagcgc
      541 aagtcccagc gcccgcggcg cacccagcgt ccgggcgagg aagtaagtgc cgccgcaatc
      601 cggcaccagg ccgaggttgg agaacgcctg gatgaagctc gccgaacgtg ccgccacgac
      661 cacgtcgcag gccagggcga tgctcgctcc cgcgccggcc gccacgccgt tcacggcgca
      721 cagcaccggc aaggggaggg cgcgcaacgc gagcacgaac gggttgtagt tggcctcgag
      781 cgacgcgccg agatcgcgcg gcgcatcgcg ctgctcatca cctggggcga cgctgcggtc
      841 cgccaggtcc tgtccggcgc agaatgcgcg accggcgccg gtgaggatca tggcgcgcac
      901 cgtgcggtgg ctgccgatgc gcgcgagcac ctcgcgtatc tcggcgtgca tggtgtcgtt
      961 gaaggcgttg aggcgctcgg gacggttcag ggtgagcgtg gccaccccct cgctcacctc
     1021 cagggcgacg ctgcgaaagg ctgatgccaa ggtgcagttc tccaatggac ggccggggcc
     1081 cgggcgttta tcggtcgtca ccgggcattt atcgaccgtt cggtcgacga atgttagggg
     1141 gaatcgccgt tcgagacaag tactttcaag acaagggctt ccgagtcccc ccgcgggcgt
     1201 ggctgctgcg gcgcggcaag ggcctgaacg ttgcttaccc ggccggtata cacccgcgtg
     1261 ggcggaggag ttgccagatg atgctgagcg aaatgatgca ggaggcgctc gagcagggga
     1321 ttcggcaggt cagggcggcc ggctcccgct tgcatccgtt cctggtggtc gagccggcca
     1381 agatcctgtt tctcttcgac ccgtccggcc agagcgaacc gatggagatg gcgctatgcg
     1441 cgatccgcga gcacgccccg gcgtgcgaac gcgtagtgct ggtgctcaac accacgctga
     1501 ccgcgcggga cggacgcaag tccgacgccg tcgtggcaat gggctcggaa cgcggccagg
     1561 agaccggtcc gatgtgggcg cagatctatc ggcccggaag ctggttccgg cgtttccgcg
     1621 tcgagggcga gcgcgaaacg atcggcgagg tcaagaacct gatccgggaa gcgctcgccg
     1681 cctgacgcag cggcatcact tcggcgccaa cttcacttcg gggctggggt cggccccagc
     1741 accttctcga tcgccatgcc gatggcgagc aagcgcgtgt cgctgccgcg cggaccgtcg
     1801 atctcgatgc cgaccggcaa tcccgtcgtc gggcccaatc cggccgggat cgacagaccg
     1861 ggcactccgg cgttgctgcc gggatcggtg ttctggatga acagcagcat ggtctcgagc
     1921 ttgctggcgt ccgggccctg caccgccgcc acgtgcggcg tggtcggaaa aatcagcgcc
     1981 tcgatgccgt acttggcgaa cgtgtccgca tagtgcttga tcagcgcggg acgtgcctgc
     2041 ttgatggctg cctcgtacac cggcgccgca tcgatcaggc cgtcgggcgt gggcagcttg
     2101 cgcggcacca ccagcgcgct gtaggtaccc ttcacatcct ggctggcgat catcgccgcc
     2161 aactcctcga ccgtcttgcc ggtctgatac ttgtcgagat aggccttcat gtcgtcgtag
     2221 gcctcgtaca gcgcaaccgg gaagctggcc ccgtcgttga gcttctgcag ctcgggcatg
     2281 tccacctcga cgatctccgc ccccgccttc gccagcttgt ccagcgctgc gttcatcacg
     2341 ctgccggtat ccgcatccag gttgttgaag aagtaggcgc ggtacacgcc gagcttcacg
     2401 cctttcagcg acgccggcgc cggcaccttg ccgccggcga tcacggcgtc gagcacagcc
     2461 acgtcggcga cggtgcgcgc catcgggccc accgtgtcgc gcgtatgcga gatcggggcc
     2521 acgcccgcgt tgtcgtagcg gtcgatggtc ggacggaagc cggcgccgcc ggtcaccgcc
     2581 gaggggatgc ggctggagcc gccggtgtcg ctgcccaggc cgccgggggc gatgcgggcg
     2641 gcgatggcgg cggcggtgcc gctggagctg ccgccggcga agcgcgtgcg gtcgtaaggg
     2701 ttgcgcacgc cgatcggctg ggcgccgaaa gtcgcctcgt tgtagccgga ga
//
LOCUS       DASPGE010000303         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_79061, whole genome shotgun sequence.
ACCESSION   DASPGE010000303 DASPGE010000000
VERSION     DASPGE010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79061"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /gene="soxX"
                     /locus_tag="VD791_09740"
     CDS             <1..611
                     /gene="soxX"
                     /locus_tag="VD791_09740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HXV08299.1"
                     /translation="CLLVLGALAALSLPSRAGDVSAQAEAVWRKSFSSASPEEWAARL
                     AQDEVNALCSKHRNNPPPEAAARIIELSQASLELPEDGKLMGDWKEGEKLASIGTGGH
                     IGRIQPDRPGTRRGGNCYACHAIAKKEIAAGNLGPSLNNYAKVRGTSPEAVRYVYAKI
                     YNAQAFVACSSMPRFGHNRWLTPKEIADAVAFLLDPESPVNK"
     gene            660..>2342
                     /gene="soxB"
                     /locus_tag="VD791_09745"
     CDS             660..>2342
                     /gene="soxB"
                     /locus_tag="VD791_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HXV08300.1"
                     /translation="MNRRDFLQLLAAGAAAGLVLDRREALAGEGGALYDVPRFGNVSL
                     LHFTDCHAQLNPIHFREPSVNLGVGAAAGKPPHLVGEALLAHFGIAPGSPQAHAFTCL
                     DFAQAAQTYGKVGGFAHLATLVKRLRAQRPGALLLDGGDTWQGSATALWTAGQDMVDA
                     CLALGVDVMTAHWEFTYGAQRVQQIVDNDFKGRVDFVAQNVKTADFGDPVFPAYVIRE
                     VNGVPVAILGQAFPYTPIANPRHFVPEWSFGIQEESLQPAIDRARAEGAQVVVLLSHN
                     GMDVDLKLAGRMRGLDAILGGHTHDGVPAPTLVSNAGGKTLVTNAGSNGKFLAVLDLE
                     VKGGKVADYRYRLLPVFANLLPADAQMTALIARQRAPYAQKLAEPLAVSEGLLYRRGN
                     FNGSFDQVILDALIAVKGAQIALSPGFRWGTSLLPGETITREHVMDQTAITYPHVTLT
                     DMSGETIKAVLEDVADNLFNPDPYYQQGGDMVRVGGLAYTCEPRAKMGERIRDMRLVD
                     TPIEPTKTYRVAGWAPVAEGASGEPIWELVERHLRARRSVAPPKLNLPRLIGV"
BASE COUNT          386 a          798 c          805 g          353 t
ORIGIN      
        1 gctgccttct cgtgctgggg gcacttgcgg cgctgtcgct gccctcgcgc gccggcgatg
       61 tttccgcaca ggccgaagcc gtctggcgca agtcgttctc cagcgccagc ccggaggagt
      121 gggccgcgcg cctggcgcag gacgaagtca acgcgctgtg cagcaagcac cgcaacaatc
      181 cgccgcccga ggccgccgcg cgcatcatcg agctgtcgca ggccagcctc gagctgcccg
      241 aggacggcaa gctgatgggc gactggaagg agggcgagaa gctcgcctcc atcggcaccg
      301 gcggccacat cggccgcatc cagcccgacc gcccgggcac gcgtcggggc ggcaactgct
      361 acgcctgtca cgccatcgcg aaaaaggaga tcgccgccgg caatctgggc ccgagcctca
      421 acaactacgc caaggtgcgc ggcacctcgc cggaagcggt gcgctacgtg tacgccaaga
      481 tctacaacgc gcaggccttc gtcgcgtgct ccagcatgcc gcgcttcggc cacaatcgct
      541 ggctcacgcc caaggagatc gccgatgcgg tggcgttcct gctcgatccc gagtcgccgg
      601 tgaacaagtg aagaactcgt gaatcgtgat tcgtcgtgat tcgtgaatcg taacagccga
      661 tgaatcgaag ggatttcctg cagttgctcg cggctggcgc tgcggccggc ctcgtgctcg
      721 accggcgcga ggcgctcgcc ggggagggcg gtgcgttgta cgacgtgccg cgcttcggca
      781 acgtcagcct gctgcacttc accgactgcc atgcgcagct caacccaatc cactttcgcg
      841 agccgagcgt caatctgggc gtcggcgcgg cggccgggaa gccgccgcac ctggtcggcg
      901 aagcgctgct ggcgcacttc ggcatcgcgc ccggctcgcc gcaggcgcat gctttcacct
      961 gcctggactt cgcccaggcg gcgcagacct acggcaaggt cggcgggttc gcgcatctcg
     1021 cgacgctggt gaagcggctg cgggcgcagc ggcccggggc gctgctgctc gacggcggcg
     1081 acacgtggca gggctcggcc acggcgttgt ggacggcggg gcaggacatg gtggacgcct
     1141 gcctcgcgct gggcgtggac gtgatgaccg cgcactggga gttcacctac ggcgcgcagc
     1201 gcgtgcagca gatcgtcgac aacgacttca aaggcagggt cgacttcgtc gcacagaacg
     1261 tcaagaccgc cgacttcggg gatccggtgt ttcccgccta cgtgatccgc gaagtcaacg
     1321 gcgtgccggt agcgatcctc ggccaggcgt tcccctatac cccgatcgcc aatccgcgcc
     1381 acttcgtgcc ggagtggagc ttcggcatcc aggaagagag cctgcaaccg gcgatcgatc
     1441 gggcgcgcgc ggaaggcgcg caggtcgtgg tgctgctctc gcacaacggt atggacgtgg
     1501 acctgaagct cgccggccgg atgcgtgggc tggatgcgat cctcggcggc catacgcacg
     1561 acggcgttcc ggcgccgacc ctggtgagca atgcgggcgg caagacgctg gtcaccaacg
     1621 ccggctccaa tggcaagttc ctggccgtgc tcgacctcga ggtcaagggc ggcaaggttg
     1681 ccgactaccg ctatcggctg ctgccggtgt tcgccaacct gctgcccgcc gatgcgcaga
     1741 tgacagcgtt gatcgcccgg cagcgggccc cgtacgcaca gaagctggcc gagcccctgg
     1801 cggtgagcga gggcctgctg taccggcgcg gcaacttcaa cggcagcttc gaccaggtga
     1861 tcctggatgc actgatcgcg gtgaaaggcg cccagatcgc gctctcgccc ggattccgct
     1921 ggggcacgtc gctgctcccg ggtgagacca tcacgcgcga gcacgtgatg gaccagaccg
     1981 cgatcaccta tccgcacgtc acgctcaccg acatgagcgg tgagaccatc aaagccgtgc
     2041 tggaggacgt ggccgataac ctgttcaatc ccgatcccta ctatcagcag gggggcgaca
     2101 tggtgcgcgt gggcgggctc gcctacacgt gcgagccgag ggcgaagatg ggcgagcgca
     2161 tccgcgatat gcgcttggtc gacacaccga tcgagccgac caagacctac cgcgtcgccg
     2221 gctgggcgcc ggtcgccgaa ggcgcaagcg gcgagccgat ctgggagctc gtcgagcgcc
     2281 acctgcgcgc gcggcgctcg gtcgcgccgc cgaaactgaa ccttccgcgc ctgatcggcg
     2341 tc
//
LOCUS       DASPGE010000304         4060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1484011, whole genome shotgun sequence.
ACCESSION   DASPGE010000304 DASPGE010000000
VERSION     DASPGE010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484011"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1800
                     /locus_tag="VD791_09750"
     CDS             <1..1800
                     /locus_tag="VD791_09750"
                     /inference="COORDINATES: protein motif:HMM:NF027510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HXV08301.1"
                     /translation="TENAPAVVIDNGITVSDVDDTQIAGATVAISAGLTAGDVLGFTD
                     TATITGVYNAGTGVLTLTGTDTLAAYQAALRSVTYSSTSDDPTASSASRTIAWQVTDA
                     NSDGTGAQTSAPVTSTVNLAALADAPVVTAGAVTGYTENAAAVAIDNTITVSDADDTQ
                     IAAATVTISAGLSAGDVLGFSNTVNISGVYNAGTGVLTLTGTDTLAAYEAALRSVTYS
                     STSDDPTASSASRTISWQVTDANSDAAGAASSVAVASTMNVTAVNDAPVLGANALTLD
                     QGDSVLFNPSILSASDVDNPAGALSFLVGNVTSGQFELISAPGVAVTSFTQAEVLAGQ
                     VRFVHDGNPVVPGYDVRVSDGTLTIGPFAASVTFNPTPVTVSTPPPAPPPEPPPFTIE
                     PAPAPAPEPAAEVAPTSGEPGDALTDAGFSPGRVLANFNDLTEISVRLAAAEARVPLK
                     VAPPPESNPNRYEARPSVEPQVELLDMLPAELSFTPSTPLDWVATSAFEESAEEQIRG
                     ELEVLLDSVKFGGMALSVGVVWWASRISGLLGTLIASTPAWRHIDPLPVLGRDDKDED
                     KWYEPDDSEADANELAVSLVLDGGRRDASDSDE"
     gene            1948..3030
                     /locus_tag="VD791_09755"
     CDS             1948..3030
                     /locus_tag="VD791_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HXV08302.1"
                     /translation="MAYKVLVLGTGHIGRAIAHLLSHAQGQPSYDVTVADREVAAELR
                     SAFDWREVDADNGAFTRLLHGYDMVVNALPFHLAGCVAHAAHGARVHYFDLTEDVAAT
                     REIRALAAEAATAFMPQCGLAPGFIGIAGYALSREFDKLERLHMRVGALPQYPANALK
                     YNLTWSVDGLINEYCHPCEAIVEGRRIELPPLEGYETFALDGVHYEAFNTSGGLGTLA
                     ETLEGEVQYLDYKTIRYPGHRDIAKLLLQDFGLAGKRDMMKDILHAAIPATAQDVVVV
                     FVTASGLRRGQRTQQSFARKVYAQSWSGVQMTAIQITTASGVCAVLDLFAQGRLQRTG
                     FVRQEDVPLAEFLGNRFGRAYEAAAA"
     gene            3096..>4060
                     /locus_tag="VD791_09760"
     CDS             3096..>4060
                     /locus_tag="VD791_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXV08303.1"
                     /translation="MGLHTDTQAILKSLGANAGDSAGGVIAVSPIDGERIGGVRADDA
                     ASVAAKALQAERAFLEWRGVPAPGRGELVRRFGEIVRAHKEPLGRLVSLESGKILQEG
                     LGEVQEVIDICDFAVGLSRQLHGLTIASERPGHRMMETWHPLGPAAVITAFNFPVAVW
                     GWNAALALVCGDSVVWKPSDRTPLTALAMQALFERACAGYQSAPAHLNQLVIGGTDQG
                     RALLDAPQIRLVSATGSTRMGRAVAAQVAPRFLRTILELGGNNGAIVAPSADLTLAER
                     AILFAAVGTAGQRCTTLRRLIVHRSLYDELLGRLQRLFQRVSIGDP"
BASE COUNT          635 a         1410 c         1441 g          574 t
ORIGIN      
        1 accgagaatg cgccggcggt ggtgatcgac aacggcatca ccgtgagcga cgttgacgac
       61 acacagatcg ctggcgcgac ggtcgccatc tccgcaggac taaccgccgg ggatgtgctg
      121 ggcttcaccg ataccgcgac catcaccggc gtgtacaacg ccggcaccgg ggtgctcacg
      181 ctgaccggca ccgacacgct ggccgcctac caggcggcgc tgcgctcggt gacctacagc
      241 tcgacgagcg acgacccgac ggccagcagc gccagccgca ccatcgcctg gcaggtcacg
      301 gatgccaact ccgacggcac cggagcgcag acaagcgcgc cggtcaccag cacggtgaac
      361 ctcgctgcgc tggccgacgc gccggtggtc accgcaggtg cggtcacagg ctacaccgag
      421 aacgcggcgg cggtggcgat cgacaacacc atcaccgtga gcgacgcgga cgacacccag
      481 atcgccgcgg ccacggtgac gatctccgcg gggctgagtg ccggcgacgt gctgggcttc
      541 agcaacacgg tcaacatcag cggggtgtac aacgcgggca ccggggtgct cacgctgacc
      601 ggtaccgaca cgctggcggc ctatgaggcg gcgctgcgct cggtgaccta cagctcgacg
      661 agcgacgacc cgacggccag cagcgccagc cgcaccatca gctggcaagt gaccgacgca
      721 aactccgacg ccgcgggtgc ggccagcagc gttgcggtcg ctagcacgat gaacgtgacg
      781 gccgtcaacg atgcgccggt cctgggggcc aatgcgctga ctctcgatca gggcgacagc
      841 gtgctgttca atccgagcat cctgagcgcc agcgacgtgg acaatcccgc cggcgccttg
      901 agcttcctgg tcggcaacgt caccagcgga cagttcgaac tgatcagcgc gccaggcgtc
      961 gcggtcacga gcttcacgca agccgaggtg ctggccgggc aggttcgttt cgtgcacgac
     1021 ggcaatcccg tggtcccggg ttacgacgtg cgggtgagcg acggtacgct gaccatcggt
     1081 cccttcgccg caagcgtgac cttcaacccg accccggtga cggtaagcac gccgccgccg
     1141 gcaccgccgc cggagccccc gccgtttacg atcgaacccg cgcctgcgcc ggcgcccgag
     1201 ccggccgcgg aggtcgcgcc caccagcggc gagccgggcg atgcgctgac cgacgcgggc
     1261 ttctccccgg ggcgggtgct ggccaacttc aacgacttga ccgagatctc ggtgcgcctg
     1321 gcggcggcgg aggcgcgcgt gccgctcaag gtggcgccgc cgccggagtc caatccgaac
     1381 aggtacgagg cgcggccctc ggtcgagccg caggtcgagc tgctcgacat gctcccggca
     1441 gagctgtcct tcacgccttc gacgccgctc gactgggtgg ccacttcggc cttcgaagag
     1501 agcgccgagg agcagatccg cggcgagctc gaagtgctgc tcgattcggt gaagttcggc
     1561 ggcatggcgc tgtcggtggg agtggtgtgg tgggcgagcc gcatcagcgg gttgctcggc
     1621 acgctgatcg ccagcacacc ggcgtggcgc cacatcgacc cgctgccggt gctggggcgc
     1681 gacgacaagg acgaggacaa gtggtacgag cccgacgaca gcgaggccga cgccaacgag
     1741 ctggccgtgt cgctggtgct cgacggcggc cgccgcgacg caagcgatag cgacgagtaa
     1801 gagcccctct ccggggcagg cacgctttct gctcgggctt cgccggttcg ggccgtcctc
     1861 ttgtgcaatg acggcgaggg gagggtccgc attcgttccc gttgcctgcg ccgcgccgcg
     1921 gcgcagacgg cgccggaggg ctgccccatg gcatacaagg tcctcgtcct gggcaccggc
     1981 cacatcggcc gcgctatcgc ccatctgctg tcccatgccc agggacagcc cagctacgat
     2041 gtcaccgtcg cggaccgcga ggtcgcggcc gagctgcgca gcgcgttcga ctggcgcgag
     2101 gtcgacgccg acaacggtgc gttcacgcgc ctgctgcacg gctacgacat ggtggtcaac
     2161 gccttgcctt tccacctcgc cgggtgcgtg gcgcacgccg cccacggcgc acgcgtgcac
     2221 tacttcgacc tcaccgagga cgtggccgcc acccgcgaga tccgcgccct cgccgcggag
     2281 gccgccacgg cgttcatgcc gcagtgcggg ctggcgcccg gcttcatcgg catcgccggc
     2341 tacgcgctgt cgcgggagtt cgacaagctg gagcggctgc acatgcgcgt gggcgcgctc
     2401 ccgcaatacc cggccaatgc gctgaagtac aacctcacct ggtcggtgga cgggctgatc
     2461 aacgaatatt gccatccgtg cgaggcgatc gtggaggggc gccgcatcga gctgccgccg
     2521 ctggaagggt acgagacctt cgcgctcgac ggcgtgcact acgaggcatt caacacctcc
     2581 ggcgggctgg gcaccctggc cgagacgctg gagggcgagg tgcagtacct cgactacaag
     2641 accatccgct accccggcca tcgcgacatc gccaagctgc tgctgcagga cttcggtctg
     2701 gcgggcaagc gcgacatgat gaaggacatc ctgcacgcgg ccattccggc cacggcgcag
     2761 gacgtggtgg tggtgttcgt caccgcttcg gggttgcggc gcggccagcg gacgcagcaa
     2821 tccttcgcgc gcaaggtgta tgcgcagagc tggagcgggg tgcagatgac cgccatccag
     2881 atcaccaccg caagcggggt ttgcgccgtg ctcgatctgt tcgcgcaggg gcggctgcag
     2941 cgcacgggat tcgtgcgcca ggaggacgtg ccgctggcgg aatttctcgg caaccgcttc
     3001 ggccgcgcct acgaggcggc cgcggcgtag actgcgcgcc acggccttgg ggcgcgggcc
     3061 ggcggctcgc acgagaggag cagtggagaa gcgatatggg cctgcacacc gacacccaag
     3121 cgattctgaa gtcccttggc gcgaacgcag gcgacagcgc cggtggcgtg atcgcggtga
     3181 gcccgatcga cggcgagcgc atcggcggcg tgcgagcgga cgatgccgcc tccgtggcgg
     3241 ccaaggcgct gcaggccgag cgcgccttcc tcgaatggcg cggcgtcccg gcgcccgggc
     3301 gcggcgagct ggtgcgccgg ttcggcgaga tcgtgcgcgc gcacaaggag ccgctcgggc
     3361 gcctggtgtc gctcgagagc ggcaagatcc tgcaggaagg gctgggtgaa gtgcaggagg
     3421 tgatcgacat ctgcgacttc gcggtcggcc tgtcgcgtca gctgcacgga ctgaccatcg
     3481 ccagcgagcg ccccggccat cgcatgatgg agacctggca cccgctgggg cctgcggcgg
     3541 tgatcaccgc cttcaacttt ccggtcgcgg tgtggggctg gaatgccgcg ctcgccctgg
     3601 tgtgcggcga cagcgtggtg tggaagcctt cggaccgcac cccgctcacc gcgctggcga
     3661 tgcaggcgct gttcgagcgt gcttgcgccg gttaccagtc cgcgcccgcc catctcaacc
     3721 agctcgtcat cggcggcacg gaccagggca gggcgttgct ggacgcaccg cagatccgcc
     3781 tggtgtcggc gaccggctcg acccgcatgg gacgtgccgt ggcggcgcag gtggcgccgc
     3841 gcttcctgcg caccatcctc gagctgggcg gcaacaacgg cgcgatcgtc gcgccctccg
     3901 ccgacctgac tctggccgag cgcgccatcc tgttcgccgc ggtaggcacg gcggggcagc
     3961 gctgcaccac gttgcgccgg ctgatcgtgc accgcagcct gtacgacgag ctgctgggac
     4021 gcctgcagcg gctcttccag cgcgtgagca tcggcgaccc
//
LOCUS       DASPGE010000305         7371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1719421, whole genome shotgun sequence.
ACCESSION   DASPGE010000305 DASPGE010000000
VERSION     DASPGE010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7371
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719421"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VD791_09765"
     CDS             <1..247
                     /locus_tag="VD791_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXV08304.1"
                     /translation="EDALGLAEALHGARDVASALRDYERARRGYGARVVARARHLGAY
                     MQAQVRTAAERAMAERYRTPQAVLRETATPMATWGAP"
     gene            432..1403
                     /locus_tag="VD791_09770"
     CDS             432..1403
                     /locus_tag="VD791_09770"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HXV08305.1"
                     /translation="METTNWAFPEELQPKPEEVRFDLARALDAVVMLRAEVPEDAFTA
                     AILGTERLGNGVVIREDGLVLTIGYLVTEARSVWLTTNDGGVVEGYPIAYDFATGFGL
                     VQPLGRLDAPALQRGSARSCQVGGNAIVIGHGGAEHALKARIIDKREFAGYWEYVLDE
                     ALFTAPAHPQWGGSALVAADGRLAGIGSLLVDEAVEGEQLQANMFVPLDALEPILDDL
                     LTRGQSARPPRPWLGMYTTEANKQLVVAGVAPGGPAARAGVRQGDVVLQVAGERAGGL
                     AELFRSIWRLGPAGVEVPLTLVRAGAQVQVRLRSADRSDFLKKPQLH"
     gene            complement(1430..2389)
                     /locus_tag="VD791_09775"
     CDS             complement(1430..2389)
                     /locus_tag="VD791_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXV08306.1"
                     /translation="MPQSVLIIGANGRLGRVLASAFADAGWQVAAHVRREPAGHARPG
                     VRFVRAALGDPAALAEECGRADVVVHAANVPYTRWRRDAMPLAQGAVDTARRLNAVLM
                     FPGNVYNFGARMPPLLEEDTPQEPTARKGRLRVEVEQALREAARSGLRVAVLRAGDFF
                     GGPGRGAWFDLMMAKSLRSGRVVYPGRTDVVHAWAYLPDLARAFVMVAERRAALAQFD
                     VLHFPGHGVSGAQMIECIGAAAHRLRLIDAQTRLQTAGIPWLALRAGGLVVPMWREVA
                     ELRYLWYAPHRLSGERLAALIGGIPHTPLERAVEEALRELFQR"
     gene            2487..3785
                     /locus_tag="VD791_09780"
     CDS             2487..3785
                     /locus_tag="VD791_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXV08307.1"
                     /translation="MDAARQTELLSLAGKYLAGGGTGLFVLPPELNLVVARGEGSRVW
                     DVAGREYIDYHLSSGPALLGHGHPAITEAVSAQLPKGTTYFFLNEPAIRLAQRLVDAI
                     PCGETVHYTGSGTEATFYALRIARAWTRRNKVLKFEGAWHGMHDYGLWGTVPGTPSDY
                     PRAAPDSVGVPRETGETVLVTPFNETQRAVEMIERHAGELAAVLVEPLQRVLQPEPGF
                     LQAVREVTKKHGIVLIFDEIVTGFRIAWGGAQERYGVVPDLACYGKAISGGFPMAAIV
                     GSAQIMSVLDARSRPRAEVVWATNTLNGNPVCAAAGLAALDVLSRPGVYQGLQRIGTR
                     LRQGMVEAGARHGFAVQAPGEDAVWGIRFTDRRPLRTWIDLTTHDKDLGWRWAIELMK
                     RGLLVNPNEKFYISIAHSEADVQRTLAIVDEAFAALRKQG"
     gene            3983..5128
                     /locus_tag="VD791_09785"
     CDS             3983..5128
                     /locus_tag="VD791_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08308.1"
                     /translation="MKRHIRAAALAVCATALLLHAAAATARATPEQVGRQIEQAFNTQ
                     DTDTLVQLIDLQALSSEVMSDLGLSAEQAGKLREGMRRSLRTNIEAGLRNFAQREGVA
                     KFMRTGKQAGKTYSLVRMEYQAGDGGFDYIEYYLTPAGLIEDWYTHTRGSRASTAMRL
                     AVSAMLDKGSMLASLFGVRSVDGQEVERFRVFSRQLAAGDLPAAYRALDGLPESYKRT
                     KDWAMLRASISSHDEVAYRSSLAHLAKHFGDDPSVQFMLIDHYFYEGRFDQAYKAVSA
                     FEAHVGEDGVTNFLKCSCLIGWKRYDDAVAACQRSITVEADFKSGYWGTITAGIEGGN
                     PKVALAALSAYEQAFQVSFDPDVLAKTDPYRTIAGTPEFAAWAKLRR"
     gene            5342..7324
                     /locus_tag="VD791_09790"
     CDS             5342..7324
                     /locus_tag="VD791_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXV08309.1"
                     /translation="MHAAAPSEADTFPKLLLEHARLRGAATAMREKDLGIWQAWSWSE
                     VAESVRDLAGGLSALGLRRGENLAIIGDNRPQLYWAMSAAQALGAVPVPLYQDAVADE
                     MAYILDDADIRYAVVEDQEQVDKLLEIKHRLPKLETIIYEEPRGMRNYAQPFLHRYAE
                     VQRLGRKLHEDHPAFLQREVDQGAGQDVAIMLYTSGTTGKPKGVCHTHAALISAARGG
                     CEFDRLGPGDEVLSYLPMAWVGDNLFSYAQALAGGFTINCPESGDTVMTDMREIGPTY
                     YFAPPRVYENLLTQVMIRMEDASGVKRALFHYFLGVARRCGAEILDRAPGVSVVDRLL
                     YGLGRLLVYGPLRNVLGMSRIRVAYTAGAAIGPDLFRFYRAIGVNLKQLYGSTETCAY
                     VCLQPDGKIKLDSVGPPAPGVEVQLAADGEVLVRSRAMLKEYYKRPDATAEVFDEKGF
                     FRTGDAGFFDQDGHLKIIDRAKDVGRLANGAIFAPNYIENKLKFFPQIKEAVAFGHGR
                     NEVCAFVNIDLGAVGNWAERRGLAYAGYTDLAGKPEVYDLIQECVEKINAELAADPMV
                     ADSQVHRFLVLHKELDPDDDELTRTRKVRRGFIAEKYAPLVEALYAGKRSCHIETEVK
                     FEDGRRGKIAADVQIRQVQTFPSAPTTPAVTQRKAA"
BASE COUNT         1173 a         2570 c         2577 g         1051 t
ORIGIN      
        1 cgaggatgcg ctcgggctgg ccgaggcgct gcatggagcg cgcgacgtcg cgagcgcgct
       61 gcgcgattac gaacgcgcgc gccgcggcta cggcgcgagg gtcgtcgcgc gcgcgcggca
      121 cctcggcgct tacatgcagg cccaggtgcg caccgccgcc gagcgcgcga tggccgagcg
      181 ctaccgcacg ccgcaagccg tcctgcgcga gaccgccacg ccgatggcga cctggggtgc
      241 tccttgacat taaacgtgat aggcggcgca gtagcgcggc ctatcaagcg agcggcgcca
      301 actgccgtta aacgggaagc gatgccgttg cacgccggcc ggcgctgttt ctgaaaaggc
      361 aaacccggcg cgcggcaaat gcgccacaat cgcgtaacat tcgccacgac gccgctcgca
      421 tccgcatctg catggaaacc accaactggg ccttccccga agagctgcag cccaagcccg
      481 aagaggtgcg cttcgacctc gcgcgcgcgc tcgatgccgt ggtgatgctg cgcgcggagg
      541 tcccggagga cgcgttcacc gccgccatcc tcggcaccga gcgcctgggc aacggcgtgg
      601 tgatccgcga ggacggcttg gtgctcacca tcggctatct ggtgaccgag gcgcgctcgg
      661 tgtggctcac caccaacgac ggcggcgtgg tcgagggcta ccccatcgcc tacgacttcg
      721 ccaccggctt cgggctggtg cagccgctgg gcaggctgga cgcgccggcg ctgcagcgcg
      781 gttcggcgcg ctcgtgtcag gtcggcggca acgcgatcgt gatcggccac ggcggcgccg
      841 agcacgcgtt gaaggcgcgc atcatcgaca agcgcgagtt cgcgggctac tgggagtacg
      901 tgctggacga ggccctgttc accgcgccgg cgcacccgca gtggggcggc tcggcgctgg
      961 tcgccgcgga cggccggctg gccggcatcg gctcgctgct ggtggacgag gccgtcgaag
     1021 gcgagcagct gcaagccaac atgttcgtgc ccctcgacgc gctcgagccg atcctcgacg
     1081 atctgctcac gcgcggccag tccgcgcggc cgccgcgtcc ctggctcggc atgtacacga
     1141 ccgaggcgaa caagcagctc gtggtggccg gcgtggcccc cggcggcccg gcggcgcgcg
     1201 ccggggtgcg acagggcgac gtggtgctgc aggtggcggg cgagcgcgcg ggcggcctcg
     1261 ccgagctgtt ccgcagcatc tggcgcctcg ggccggcggg ggtcgaggtc ccgctgaccc
     1321 tggtgcgcgc cggcgcgcag gtgcaggtgc ggctgcgctc ggccgaccgc agcgatttcc
     1381 tgaagaagcc gcagctgcac tgagcgcggg cgcgtctacc cgcgccgagc tagcgctgaa
     1441 acaactcgcg caacgcttcc tccaccgcgc gctccagcgg cgtgtgggga atgccgccga
     1501 tgagcgcggc caggcgctcg ccggagagcc ggtgcggtgc gtaccacagg tagcgcaact
     1561 cggccacctc gcgccacatc gggaccacca gcccgccggc gcgcagcgcc agccagggaa
     1621 tgcccgcggt ctgcaggcgc gtctgcgcat cgatcagccg gaggcggtgc gccgcggcgc
     1681 cgatgcattc gatcatctgc gcgccgctga cgccgtggcc gggaaagtgc agcacgtcga
     1741 actgcgccag cgcagcgcgg cgctccgcca ccatcacgaa cgcgcgcgca aggtcgggca
     1801 ggtaggccca cgcgtgcacg acgtcggttc gcccgggata gaccacgcga cccgagcgca
     1861 gcgacttcgc catcatcaga tcgaaccagg ctccgcggcc cgggccgccg aagaagtcac
     1921 cggcgcgcag caccgccacg cgcagcccgc tgcgcgccgc ctcgcgcagc gcctgctcca
     1981 cttccacgcg caggcgtccc ttgcgcgcag tcggttcctg cggcgtgtct tcctcgagca
     2041 gcggcggcat gcgcgcgccg aagttgtaca cgttgccggg gaacatcagc acggcgttca
     2101 ggcgccgagc ggtgtcgacg gcgccctgcg ccaagggcat ggcgtcgcgc cgccaacgcg
     2161 tgtacggcac gttggcagcg tgcacgacca cgtcggccct gccgcactcc tccgccagcg
     2221 ccgccggatc gccgagcgcg gcgcgcacga agcgcacgcc gggccgggcg tggccggcgg
     2281 gctcgcgccg cacgtgcgcg gcgacctgcc agcccgcatc cgcgaaggcg ctcgccagca
     2341 cgcgtcccag gcgcccgttg gcgccgatga tcagaacaga ttgcggcatt tgcaaccgat
     2401 acagaaccat tccgtccgga taccggtatt gtgctcgcaa cgcacggggt agactatgcg
     2461 cccgacccca cgcaccggtg actcacatgg acgctgcccg acaaaccgaa ctgctctcgc
     2521 tggccggcaa atatctcgcg ggaggcggca ccggcctgtt cgtcctgccg cccgagctga
     2581 acctggtggt ggcccgcggt gagggcagcc gcgtgtggga cgtggccggg cgcgagtaca
     2641 tcgactatca cctgagctcc gggccggccc tgctcgggca cggccatccc gctatcaccg
     2701 aggcggtgag cgcacagctg ccgaagggta ccacctactt cttcctgaac gaacccgcga
     2761 tccggctggc gcagcgcctg gtcgacgcga tcccgtgcgg cgagacggtg cattacaccg
     2821 gctcgggaac ggaagccacg ttctacgcgc tgcgcatcgc gcgcgcctgg acgcggcgca
     2881 acaaggtgct caagttcgaa ggcgcctggc acggcatgca cgactacgga ctgtggggca
     2941 ccgtgcccgg tacgccgtcg gactacccgc gcgcggcgcc cgattccgtg ggcgtgccgc
     3001 gcgagaccgg cgagacggtg ctggtcacgc cgttcaacga aacgcagcgc gcggtggaga
     3061 tgatcgagcg gcacgccggg gagctggcgg cggtgctggt ggaaccgctg cagcgcgtgc
     3121 tccagcccga gcccggtttc cttcaggccg tgcgcgaggt gacgaagaag cacggcatcg
     3181 tgctgatctt cgacgagatc gtgaccggct ttcgcatcgc ctggggcggc gcacaggagc
     3241 gttacggcgt ggtgccggat ctggcctgct acggcaaggc gatcagcggc gggttcccga
     3301 tggcggccat cgtcggcagc gcgcagatca tgtcggtgct ggacgcgcgc agccggccgc
     3361 gcgccgaggt ggtgtgggcc accaatacgc tcaacggcaa tccggtatgc gcggccgccg
     3421 ggctcgcggc cctcgacgtg ctgagccggc ccggcgtcta ccagggcctg cagcgcatcg
     3481 gcacgcgcct gcgccagggc atggtagagg ccggtgcgcg ccacggcttc gctgtgcagg
     3541 cgccgggcga ggatgcggtg tgggggatcc ggttcaccga tcgccggccg ctgcgcacct
     3601 ggatcgacct caccacgcac gacaaggacc tcggctggcg ctgggccatc gagctgatga
     3661 agcgcggcct gctggtcaat cccaacgaaa agttctacat ctccatcgca cacagcgaag
     3721 ccgacgtgca gcgcacactc gcgatcgtgg acgaggcctt cgcggcgttg cgcaagcagg
     3781 ggtgagccgg gcgcaggcag ctccatcggt cgccgcggtc gcttcctccg ccgtagcgga
     3841 agcgcggtcc ggcctgcccg cgggggcatg gccggcgcgc gtcttgacga tcctctgacg
     3901 tttggcgacc cgcccaggcg cgggccgccg ttggcatctt cctcgcttcg tcgggtgttc
     3961 cgactccttt cgaggaacac cgatgaagcg tcacatccgc gcagccgccc ttgccgtctg
     4021 cgccacggcc ttgctgctgc acgcggccgc cgcaaccgcc cgggccacgc cggagcaggt
     4081 gggccgccag atcgagcagg cgttcaacac gcaggacacc gacaccctgg tgcagctcat
     4141 cgacctgcag gcgctgagca gcgaggtgat gagcgacctg ggcttgagcg ccgagcaggc
     4201 gggcaagctg cgggagggca tgcgccggag cctgcgcacc aacatcgaag ccggtttgcg
     4261 caacttcgcg cagcgcgagg gcgtggccaa gttcatgcgc accgggaaac aggccggcaa
     4321 gacgtacagc ctggtgcgca tggagtatca ggccggggac ggcgggttcg actacatcga
     4381 gtactacctg acgccggccg gactgatcga ggactggtac acgcacaccc gcggcagccg
     4441 cgccagcacc gcgatgcgcc tggcggtgtc cgccatgctg gacaagggat cgatgctcgc
     4501 cagcttgttc ggcgtaaggt cggtcgacgg tcaggaggtg gagcggttcc gcgtcttcag
     4561 ccgccagctc gccgccggcg atttgccggc cgcctaccgc gccctggacg gactgcccga
     4621 gagctacaag cgcaccaagg actgggcgat gctccgggcc agcatcagca gccatgacga
     4681 ggtcgcctat cgcagctcgc tcgcgcacct ggcgaagcac ttcggggacg accccagcgt
     4741 gcagttcatg ttgatcgacc attacttcta cgaagggcgc ttcgaccagg cctacaaagc
     4801 ggtgtccgcc ttcgaggcgc acgtgggcga ggacggcgtg acgaacttcc tcaagtgcag
     4861 ctgcctgatc ggctggaagc gctacgacga cgcggtcgcc gcctgccagc ggtcgatcac
     4921 cgtcgaggcc gacttcaagt cggggtattg gggcacgatc accgccggca tcgaaggcgg
     4981 caatcccaag gttgcgctgg cggcgttgtc ggcctatgag caggcgttcc aggtgtcctt
     5041 cgaccccgat gtgctggcca agaccgaccc ctaccgcacg atcgccggaa cccccgaatt
     5101 cgcggcgtgg gcgaagctgc gccgctagtc tcgatctcta cgcagttctc tccggggctc
     5161 gcaacgcggg ccccgtttct ttttgcatcg cagcaccgcc accggttgta gcgcgccccg
     5221 gatccttata caagcggcgt tcctgcatta ctggcgttcg ccgtattacc atagcggtcc
     5281 gcccggacgg cgcagtccgg cggcaccgcc caataaccag agacctgaac gcgacgacgc
     5341 gatgcacgcc gccgccccgt cggaagcgga caccttcccc aagctgctgc tcgagcacgc
     5401 gcgcttgcgc ggtgcggcga ccgccatgcg cgagaaggat ctcggcatct ggcaggcctg
     5461 gagctggagc gaggtggctg agagcgtgcg cgacctggcc ggcggcctga gcgcgctcgg
     5521 tctgcggcgc ggcgagaatc tcgcgatcat cggcgacaat cgcccgcagc tgtactgggc
     5581 gatgagcgcg gcgcaggcgc tgggggccgt tccggtgccc ctgtaccagg acgccgtcgc
     5641 cgacgagatg gcctacatcc tggacgacgc cgacatacgc tacgccgtgg tggaagacca
     5701 ggagcaggtg gacaagctgc tcgagatcaa gcaccgcctc cccaagctcg aaaccatcat
     5761 ctacgaagag ccgcgcggca tgcgcaacta cgcgcagcct ttcctgcacc gttacgcgga
     5821 ggtgcagcgc ctggggcgca agctgcatga agaccacccg gcgttcctgc agcgcgaggt
     5881 cgatcagggc gccggccagg acgtggcgat catgctgtac acctcgggca cgaccggcaa
     5941 gccgaaaggg gtctgccaca cgcacgcggc gctgatctcg gcagcccgcg gcggctgcga
     6001 attcgaccgg ctgggcccgg gcgacgaggt gctgtcctat ctgccgatgg cctgggtcgg
     6061 ggacaacctg ttctcctatg cgcaggcgct ggcgggcggc ttcacgatca actgcccgga
     6121 atcgggtgac acggtgatga ccgacatgcg cgagatcggc ccgacctatt acttcgcgcc
     6181 gccacgcgtg tacgagaacc tgctcacgca ggtgatgatc cgcatggagg acgcctcggg
     6241 cgtgaagcgc gcgctgttcc attactttct cggcgtcgcg cggcgctgcg gcgccgagat
     6301 cctggatcgt gcgcccggcg tctccgtcgt cgaccgcctg ctgtacggtc tgggcaggct
     6361 cctggtctac gggcccttgc gcaacgtgct gggcatgagc cgcatccgcg tcgcctacac
     6421 cgcgggcgcg gcgatcggtc ccgacctgtt ccgcttctac cgagcgatcg gcgtgaacct
     6481 caagcagctg tacggctcca ccgagacctg cgcctacgtg tgcctgcagc ccgacggcaa
     6541 gatcaagctc gattcggtgg ggccgccggc ccccggcgtg gaggtgcaac tggccgccga
     6601 cggcgaggtg ctggtgcgca gccgggcgat gctcaaggag tactacaagc ggcccgacgc
     6661 gaccgccgag gtgttcgacg agaagggctt cttccgcacc ggcgacgccg gcttcttcga
     6721 ccaggacggg cacctgaaga tcatcgaccg cgccaaggac gtgggtcgtc tcgccaacgg
     6781 cgccatcttc gcccccaact acatcgagaa caagctcaag ttcttcccgc agatcaagga
     6841 ggcggtcgcc ttcggccacg gccgcaacga agtgtgcgct ttcgtcaaca tcgacctggg
     6901 cgcggtgggt aactgggccg agcggcgcgg gctggcctac gccggctaca ccgacttggc
     6961 gggcaagccc gaggtctacg acctgatcca ggagtgcgtg gagaagatca acgccgagct
     7021 cgccgccgac ccgatggtgg ccgactcgca ggtgcaccgc ttcctggtgc tgcacaagga
     7081 gctggacccc gacgacgacg agctgacgcg cacgcgcaag gtgcgccgcg gcttcatcgc
     7141 cgagaagtat gcgccgctgg tggaggcgct gtacgccggc aagcgcagct gccacatcga
     7201 gaccgaggtc aagttcgagg acggccggcg cggcaagatc gcggcggacg tgcagatccg
     7261 ccaggtgcag accttcccct cggcgcccac gaccccggcg gtcacgcaga ggaaggcggc
     7321 ttgagcgcgc agctcccagc cggcgaggtc ctgctcgccg tggacaacgt c
//
LOCUS       DASPGE010000306        10189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_782789, whole genome shotgun sequence.
ACCESSION   DASPGE010000306 DASPGE010000000
VERSION     DASPGE010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10189
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782789"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VD791_09795"
     CDS             <1..890
                     /locus_tag="VD791_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXV08310.1"
                     /translation="PGGLPKRTSRALLDIEDRSVVPVIRNPRKAGEDAEAVFYFPGCG
                     SERLFGQVGLATQAMLFDIGAITVLPPGYLCCGYPQTSAGEADKGRKIITDNRVLFHR
                     VANTLNYLDIKTVIVSCGTCHDQLQHYEFDKIFPGCRLLDIHEYLLEKGVKLDGVSGV
                     RYLYHDPCHTPMKTYRPSEVVNRLTGQEVKLNERCCGESGTLAMTRPDISTQVRFRKQ
                     EEMQRDAGARRAAGHRGAITVLTSCPSCLQGLARYDRDGGTSAEYIVVEMARHLLGPD
                     WMKRYVEAANHGGIERVLL"
     gene            901..1308
                     /locus_tag="VD791_09800"
     CDS             901..1308
                     /locus_tag="VD791_09800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HXV08311.1"
                     /translation="MSGCELCENAGGEVLWEDDRCRAVWAYQPDHPGFCRVIWDRHVR
                     EMADLEPAERERIMRVVFAVEQALIAVLQPDKVNLASLGNAVPHIHWHVIPRFRDDPH
                     FPNPVWAEKLRQTPRPLPAEFAMHMRRALDQEL"
     gene            complement(1411..2739)
                     /gene="phoR"
                     /locus_tag="VD791_09805"
     CDS             complement(1411..2739)
                     /gene="phoR"
                     /locus_tag="VD791_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HXV08312.1"
                     /translation="MIKLWGRPVLGVVLISILALVLWPVLGREKALLAYCFMLLLLLG
                     HHLINLSRLHFWLAAQAPRPVPDGSGAWEEVFAGLSRLLRRQTHIESRLSAALSRFQQ
                     AGAALPEGVVVLDEADRIEWCNPRAEAYFGLDNQRDRGQQITYLIRNPQFVRYLETGS
                     FAEPLLLRPTRGGAELVLSIQLVPYGGSEKLLLARDVTRWERLETTRRDFVANVSHEL
                     RTPLTVLNGFLETLNDTARPDPEILRRALELMTEQTVRMNQLVEDLLTLSRLESAHNP
                     PREEYVDVPGLAQTLYREAQALSAKRHRIALRLDSPHGLKGSAEELHSAFGNLISNAV
                     RYTPDGGEIEIRWMTREGEPALQVRDTGIGIEPQHIPRLTERFYRVDRSRSRSTGGTG
                     LGLAIVKHVLSRHQARLEIESDPGRGSTFTAVFPAARTMPAPAKLRPEAA"
     gene            complement(2822..3508)
                     /gene="phoB"
                     /locus_tag="VD791_09810"
     CDS             complement(2822..3508)
                     /gene="phoB"
                     /locus_tag="VD791_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HXV08313.1"
                     /translation="MAATVLVVEDEPAIQELIAYNLKQAGHQPLRADSAEEALRLVQE
                     ALPDLVLLDWMLPGVSGIELARRLRSDKRTKTVPIIMLTARADEQDKLTGLDTGCDDY
                     ITKPFSPRELNARVKAVLRRRAPQMTDDVVQAGTLRLDPATHRVSANGHPVHLGPTEF
                     RLLHFLMTHPERVHSRTQLLDQVWGDHVFVEERTVDVHIRRLRKALEPSSHDGLIQTV
                     RGAGYRLSSG"
     gene            3694..4119
                     /locus_tag="VD791_09815"
     CDS             3694..4119
                     /locus_tag="VD791_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HXV08314.1"
                     /translation="MAIEGGSAPVPTEITLHQASRLLEIAFSDGKVFKLGCELLRAYS
                     PSAEVRGHGPGQEVLQTGKREVQITAIEPVGTYAVKLVFSDGHDTGLYSWDYLYELGR
                     DQDQLWRRYLRRLEEAGASRDPVVPVRQSGAGSAGWKKL"
     gene            4116..4850
                     /gene="ubiE"
                     /locus_tag="VD791_09820"
     CDS             4116..4850
                     /gene="ubiE"
                     /locus_tag="VD791_09820"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HXV08315.1"
                     /translation="MSEPTHFGFQEVPEVDKARRVAGVFDSVSARYDLMNDLMSAGLH
                     RLWKRIAVERSGARPGQRVLDVAGGTGDLARLFARRVAPKGEVVLTDINRSMLARGRD
                     RLLDAGLALPAVQSDAESLPFPDRAFDCVSIAFGLRNVTRKERALAEMRRVLRPGGRL
                     VVLEFSRPWRWLRPAYDAYSFGVLPRLGKLVVGDADSYRYLAESIRVHPDQGQLSAML
                     QAAGLERVDCLNLTGGVVALHRGFRL"
     gene            4855..5559
                     /locus_tag="VD791_09825"
     CDS             4855..5559
                     /locus_tag="VD791_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08316.1"
                     /translation="MAVVPDPFAPARWPVVALLNHLLRGQPWLRERLVPFAGRSVRLE
                     VPPLALVLAIGAQGELQPSGAAAVDASARLSPLTVLRLAAGEEGARSGVEVNGDAALA
                     AALGGVLRELRWDAAEDLSRLIGDIPAQRLVRLGQGVLAWQREALSSLLAAVGEYLVE
                     ERHVLPGREAVASWAREVDALRDDTERLSQKIDQLQRRFDQPQPPADRLEQPSDHPQR
                     RSQQRQRRGERPSRGD"
     gene            5567..7090
                     /gene="ubiB"
                     /locus_tag="VD791_09830"
     CDS             5567..7090
                     /gene="ubiB"
                     /locus_tag="VD791_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006409723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HXV08317.1"
                     /translation="MRLFRLLRILGVALRFGLDEFLLGHERVRGLRALADRALFWRRF
                     AVPRAVRLRRALETLGPIFVKFGQLLSTRRDLLPVDIADELAKLQDRVPPFDSRHVRQ
                     RIEQVYGAPIEQVFAEFDLTPVASASVAQVHFARLRDGTPAAVKILRPGIAAVIRHDV
                     ALLYSAAGLVQALWREGKRLKPREVVAEFEKHLHDELDLMREAGNASQLRRNFAGSPL
                     LRVPEVYWDYCATEVMVMERMSGTPVSQVEALRARGVDIPLLARKGVEIFFTQVFRDG
                     FFHADMHPGNIFVDADGRYVALDFGIMGTLTEVDKNYLAQNFLAFFRRDYRRVAQAHV
                     DAGWVPRDTRVDEFESAIRAVCEPIFDKPLKEISFGKVLLRLFQTSRRFNVEIQPQLV
                     LLQKTLLNIEGLGRQLDPDLDLWKTAKPYLERWMSEQMGWRGLLRRLRQEAPEWASVL
                     PQLPRLVHRALSERDARSWSGQLDELANRQRAQNRLLVAVASLLVLTVVLLLFAALG"
     gene            7275..8732
                     /locus_tag="VD791_09835"
     CDS             7275..8732
                     /locus_tag="VD791_09835"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HXV08318.1"
                     /translation="MRRNHQTRGTTLALTALVAALLGGVQPARAQTEIFDDLLEKLRD
                     KGVLSDAEYDALKQARDEERLQQRAERRKQALEAATAAEHEEKAKAEEKTTLKGRFRD
                     GFTFESGDKQHSISVTGRVHADYRSFSEDSTNANSADTFDVRRAYIGLSGKFYNDWTF
                     DVTADVAQTSAPQLDVAWLNWGRYKPIQARAGQFKMPFSLEELTSSRFIDFQERSLVN
                     SLVPAKERGAMLHGAFGKGFFYGAALSNGAGKNANEGNSIEDSKDIIGRLGVNAAEWL
                     GNKDIVLHVAGAYSTGTIPGGVSPVGGGVRTEGRGLTFFNTAAMGAATDDVDRSRTGG
                     EVSVAWGPIKAQGEYVKSNFEWTAGGVGFDQDIKAYYAEAMWLITGEKYADAYRGGAY
                     AAIKPKNAFGSGGWGAWEVGVRYTDFDAEDFAANAGLTNKATAYTVGLKWIPVTNVRF
                     YLNYVQTDFDTPVAVSGGGTTDDEKAITLRAAVYF"
     gene            8883..9968
                     /locus_tag="VD791_09840"
     CDS             8883..9968
                     /locus_tag="VD791_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV08319.1"
                     /translation="MALLEIRNVTRRFGDFIAVDEVSLSIEAGEFFCLLGPSGCGKTT
                     LLRMIAGFDAPDGGSIWLDGSDMTGIPPEARNVHTVFQSYALFPHMSVAENIAFPLKM
                     ARVDPGEIARRVNEALEDVSLAGMSGRYPNELSGGQRQRVAVARALVNRPRLLLLDEP
                     LAALDAKLKERMQLELIALQKEVGIAFVYVTHDQQEALALAHRIAVMNHGRVEQLDEP
                     SRIYGYPTNRFVADFIGTCNILEAEVLGLDGPRMRLQIAGLGQVDASRTENARAGQRG
                     VLALRPEQVAIGTRLVPSADENHFAGRVFDFLYLGDVTVYVVELPDGSRVEAMLPNSA
                     PGRAKFFEPGDAVEINWRFDAGRFLAD"
     gene            9979..>10189
                     /locus_tag="VD791_09845"
     CDS             9979..>10189
                     /locus_tag="VD791_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV08320.1"
                     /translation="MHPGRLHKWLVSGPPLAYLLIFFAIPTLIMVVASFRDPGPYGGL
                     APLFTGEPGGAGVALTTDNYARFFTS"
BASE COUNT         1563 a         3491 c         3577 g         1558 t
ORIGIN      
        1 tgcccggtgg gctgccgaag agaacctcgc gcgcgctgct cgacatcgag gaccgatcgg
       61 tggtgccggt gatacgcaat ccgcgcaagg cgggcgagga cgccgaagcg gtgttctact
      121 ttcccggctg cggctccgag cgtctgttcg gccaggtggg cctggccacg caggccatgc
      181 tgttcgacat cggcgccatc accgtgctgc cgcccggata cctgtgctgc ggctacccgc
      241 agacctcggc cggcgaggcc gacaagggcc gcaagatcat caccgacaac cgggtgctgt
      301 tccaccgcgt ggccaacacg ctcaactacc tggacatcaa gaccgtcatc gtctcgtgcg
      361 gcacgtgtca cgaccagctg cagcactacg agttcgacaa gatcttcccc ggctgccgcc
      421 tgctcgatat ccacgagtac ctgctggaga agggcgtcaa gctcgacggc gtgagcggcg
      481 tgcgctacct gtatcacgac ccctgtcata cgccgatgaa gacctatcgg ccgagcgagg
      541 tggtgaatcg gttgaccggc caggaggtga agctcaacga gcgctgttgc ggggaatccg
      601 gcacgctggc gatgacccgg ccggacatct ccacgcaggt gcgcttccgc aagcaggagg
      661 agatgcagcg ggacgccgga gcgcggcgcg ccgcgggcca ccgcggcgcg atcacggtgc
      721 tcacctcctg tccttcctgc ctgcagggcc tggcgcgcta cgaccgggac ggcggcacca
      781 gcgccgaata catcgtggtc gagatggccc gccatctgct cgggccagac tggatgaagc
      841 ggtacgtcga ggccgccaac cacggcggca tcgaacgcgt gctcttgtga ggaggcgggc
      901 gtgagcggct gcgaactctg cgagaacgcc ggcggcgaag tgctgtggga agacgaccgc
      961 tgccgggcgg tgtgggccta ccagcccgac catccgggct tctgccgcgt gatctgggac
     1021 cggcacgtcc gggagatggc cgatctcgag ccggccgagc gcgagcgcat catgcgggtg
     1081 gtgttcgccg tcgagcaggc gctgatcgcc gtgctgcagc cggacaaggt gaacctcgcc
     1141 agcctgggca atgccgtccc gcacatccac tggcacgtga tcccgcgctt ccgcgacgat
     1201 ccgcacttcc ccaacccggt gtgggccgag aagctgcgcc agaccccccg gccgctgccg
     1261 gccgagttcg ccatgcacat gcgccgggcg ctggaccagg agctgtagcc cggatgcagc
     1321 cgcgctaggc gtttgccgat gggacgggcg gctgaatccg gagtgagaaa ggtcccgtgt
     1381 cctcctggat tcggcttcgg ctgatccggg ctacgcggct tccggccgca gcttcgcggg
     1441 cgcgggcatg gtgcgtgcgg cgggaaacac cgcggtgaaa gtgctgccgc gccccggatc
     1501 gctctcgatc tccagccgcg cctgatggcg cgacagcacg tgcttgacga tcgccaggcc
     1561 caggccggtg ccgccggtgc tgcgcgagcg gctgcggtcc acccggtaga agcgctcggt
     1621 gaggcgcggg atatgctgcg gctcgatgcc gatgccggtg tcgcgtacct ggagcgcggg
     1681 ctccccctcg cgcgtcatcc agcggatctc gatctcgccg ccatcgggcg tgtagcgcac
     1741 ggcgttgctg atcaggttgc cgaacgcgct gtgcagctcc tcggcgctgc ccttcagccc
     1801 gtgtgggctg tccagccgca acgcgatgcg atgacgcttc gcagacagcg cctgggcctc
     1861 gcggtacagg gtctgggcga gcccgggcac gtccacgtat tcctcgcgcg gcgggttgtg
     1921 cgcgctttcc aggcgcgaca gcgtcagcag gtcctcgacc agctggttca tgcgcaccgt
     1981 ctgctcggtc atcagctcca gcgcgcggcg caggatctcg gggtcgggcc gggccgtgtc
     2041 gttgagcgtc tcgaggaagc cgttgagcac ggtgagcggc gtgcgcagct cgtgggagac
     2101 gttggcgacg aaatcgcgac gcgtggtctc caggcgctcc cagcgggtga cgtcgcgcgc
     2161 cagcagcagc ttctcgctgc cgccgtaggg caccagctgg atcgagagca ccagctcggc
     2221 gccgccgcgg gtggggcgca gcagcagcgg ctcggcgaag ctgccggtct cgagatagcg
     2281 cacgaactgc ggattgcgga tcaggtaggt gatctgctgg ccgcggtcgc gctggttgtc
     2341 gaggccgaaa taggcttccg cgcgcgggtt gcaccactcg atgcggtcgg cctcgtccag
     2401 caccaccaca ccttccggca gggccgcccc ggcctgctgg aagcggctga gcgccgcgga
     2461 caggcgcgat tcgatgtgcg tctgccggcg cagcaggcgc gacagcccgg cgaacacctc
     2521 ctcccacgca ccgctgccgt cgggcaccgg tcgcggcgcc tgggccgcca gccagaagtg
     2581 caggcgcgag aggttgatca ggtggtgccc cagcagcagc aggagcatga agcagtaggc
     2641 cagcagcgct ttctcgcgcc ccagcaccgg ccacagcacc agcgcgagga tggagatcag
     2701 caccacgccg aggaccggac gtccccacag cttgatcacc gggtgtcccc caggaatgcg
     2761 gcgcgcgcac cagcgcgccg gcgccggcga ttatcccaaa aaacgcgcac cgcctcggag
     2821 ctcagccgct ggagagccgg tagccggccc cgcgcacggt ctggatcagg ccgtcgtggg
     2881 aggacggctc gagcgctttg cgcagccgcc ggatgtgcac gtccacggtg cgctcctcga
     2941 cgaacacgtg gtcgccccac acctggtcga gcagctgagt gcgcgaatgc acgcgctcgg
     3001 ggtgtgtcat caggaagtgc agcaggcgaa actcggtcgg ccccaggtgc acgggatggc
     3061 cattggcgct gacccgatgc gtggcgggat cgaggcgcag cgtgcccgcc tgcaccacgt
     3121 cgtcggtcat ctgcggtgcg cgacggcgca gcaccgcctt cacccgtgcg ttcagctcgc
     3181 gcggcgagaa cggcttggtg atgtaatcgt cgcagccggt atcgaggccg gtcagcttat
     3241 cctgctcgtc cgcgcgcgcc gtgagcatga tgatggggac ggtcttggtg cgcttgtccg
     3301 aacgcagccg gcgcgccagc tcgatgcccg acacccccgg gagcatccag tccagcagca
     3361 ccaggtcggg cagggcctcc tgcaccaggc gcaacgcctc ctctgcgctg tccgcccgca
     3421 atggctggtg gcccgcctgc ttgaggttgt acgcgatcaa ttcctggatc gccggctcgt
     3481 cctcgaccac cagcaccgta gcagccatca gccttgtccc cggatcagct ccacttagca
     3541 tcgtatgaaa gcaatgttac cgctccatga caacctcgtg caccggacgg gccggttcgg
     3601 ccggttacca tccgcggggc gtcctctgta tcatctggtg gctcgcccgt ttgatcaccc
     3661 ggggtccctg cgggccccct ctggagcgtc ccagtggcaa tcgaaggcgg cagcgccccc
     3721 gttcccaccg aaatcaccct gcatcaggcc tcgcggctgc tcgagatcgc cttctccgac
     3781 ggcaaggtgt tcaagctcgg ctgcgagctg ctgcgggcgt actcgccctc tgcggaggtg
     3841 cgcggacatg ggccggggca ggaggtactg cagaccggca agcgcgaggt gcagatcacg
     3901 gccatcgagc cggtgggcac ctacgccgtg aagctggtgt tctccgacgg acacgacacc
     3961 gggctgtact cctgggacta cctgtacgag ctcggccgcg atcaggatca gctgtggcga
     4021 cgctacctgc gacggctgga ggaagcgggc gcgagccgcg acccggtggt gccggtgcgc
     4081 cagtcggggg cgggcagcgc cggctggaaa aaactgtgag cgagcccacg cacttcggtt
     4141 tccaggaggt gcccgaggtc gacaaagcgc gccgggtggc cggcgtgttc gacagcgttt
     4201 ccgcgcgcta tgacctgatg aacgacctca tgtccgccgg gctgcaccgc ctgtggaagc
     4261 gcatcgcggt ggagcgcagc ggagcgcggc cggggcagcg ggtcctcgac gtggcgggcg
     4321 gcaccggcga cctggctcgc ctgttcgcgc gccgcgtggc cccaaagggc gaagtggtcc
     4381 tgaccgacat caaccgcagc atgctggcgc gcggccgcga ccgactgctg gacgccgggc
     4441 tggcgctccc ggcggtccaa agcgacgccg agagcctgcc cttcccggac cgcgccttcg
     4501 actgcgtgag catcgccttc gggctgcgca atgtcacgcg caaggaacgc gcgctggccg
     4561 agatgcggcg cgtgctgcgt ccgggcggcc gcttggtggt gctggagttc tcccgcccct
     4621 ggcggtggct gcggccggcc tacgacgcct attccttcgg tgtcctgccc cggctgggga
     4681 agctcgtggt cggcgacgcg gacagctatc gctatctggc cgaatcgatc cgggtgcacc
     4741 ccgaccaggg gcagctcagc gcgatgctgc aggccgccgg gctggaacgg gtggactgcc
     4801 tcaatctgac cggcggcgtg gtggccttgc accgcggctt ccggctgtga ccccgtggcg
     4861 gtagtccccg accccttcgc gcccgccagg tggcccgtcg tcgccctgct gaaccatctg
     4921 ctgcgcggcc aaccctggct gcgcgaacgg ctggtgccgt tcgccggccg cagcgtgcgg
     4981 ctggaggtcc cgccgctggc gctggtgctg gcgataggcg cgcagggaga gctgcaacca
     5041 tccggcgcgg ccgcggtgga cgcgagtgcc cggctgtccc cgctgacagt cttgcggctg
     5101 gcggcggggg aagagggtgc gcgctccggc gtcgaggtga acggcgacgc ggccctggcc
     5161 gccgccctgg gcggagtgct gcgcgagctg cgctgggacg ccgccgagga tctgagccgg
     5221 ctgatcggcg atatcccggc gcaacgcctg gtgcgcctgg ggcaaggggt gctggcgtgg
     5281 cagcgcgagg cgctgtcgag cctgctcgcc gcggtgggcg agtacctggt cgaagagcgg
     5341 cacgtcctcc cggggcgcga agcggtggcg agctgggcgc gcgaagtgga cgcgctgcgc
     5401 gacgacacgg aacggctgag ccagaagatc gatcagctac agcggcggtt cgatcagcca
     5461 cagcccccgg ccgatcggct cgagcagccc agcgatcacc cccagcggcg cagccaacag
     5521 cgacagcggc gcggcgagcg gccgagccgc ggggactgag gccggcgtgc gcctgttccg
     5581 gctgctgcgc attctcggcg tggccctgcg gttcggtctg gacgagttcc tgctcgggca
     5641 cgaacgggtt cgcgggctgc gcgcgctggc cgatcgggcg ctgttctggc gccgcttcgc
     5701 cgtcccgcgt gccgtgcgtc tgcgccgcgc gctggaaacc ctgggtccga tcttcgtcaa
     5761 gttcgggcag ctgctctcca cgcgccgcga cctgctgccg gtggacatcg ccgacgagct
     5821 ggccaagctt caggaccggg tgccgccgtt cgattcccgc cacgtgcggc agcgcatcga
     5881 gcaggtgtac ggcgcgccga tcgagcaggt gttcgccgaa ttcgacctca ccccggtggc
     5941 cagcgcctcg gtcgcgcagg tgcatttcgc ccggctgcgc gacggcacgc cggcggcggt
     6001 gaagatcctg cgacccggca tcgccgcggt gatccgccac gacgtcgcgc tgctctacag
     6061 cgcggcggga ttggtgcagg ccctgtggcg cgagggcaag cgcctcaagc cgcgcgaggt
     6121 ggtggcggag ttcgagaagc atctccacga cgagctcgat ctgatgcgcg aagcgggcaa
     6181 cgccagccag ctgcggcgca acttcgccgg ctcgccgctg ctgcgcgtgc cggaagtgta
     6241 ctgggactac tgcgccaccg aggtgatggt gatggagcgc atgagcggca cgccggtgag
     6301 ccaggtggag gcgctgcgcg cccgcggcgt ggacatcccg ctgctggcgc gcaagggtgt
     6361 cgagatcttc ttcacacagg tgttccgcga cggcttcttc catgccgaca tgcaccccgg
     6421 caacatcttc gtcgacgctg acgggcgcta cgtggcgctc gacttcggga tcatgggcac
     6481 gctcaccgag gtggacaaga actacctggc gcagaacttc ctggcgttct tccgccgcga
     6541 ctaccggcgc gtggcgcagg cgcacgtgga cgccggctgg gtgccccggg acacgcgcgt
     6601 ggacgagttc gagtcggcga tccgcgcggt gtgcgaaccg atcttcgaca agccgttgaa
     6661 ggagatctcc ttcggcaagg tgctgctgcg cctgttccag acctcgcgcc gcttcaacgt
     6721 ggaaatccag ccgcagctgg tgctgctgca gaagacgctg ctcaacatcg agggcctcgg
     6781 gcgccagctc gaccccgacc tcgatctgtg gaagaccgcc aaaccctatc tcgagcgctg
     6841 gatgtccgaa cagatgggct ggcgcgggct gctgcgtcgc ctgcgccagg aagccccgga
     6901 gtgggcctcg gtgctgccgc agctgccgcg cctggtgcat cgtgccctgt ccgagcgcga
     6961 cgcgcgttcc tggagcgggc agctcgacga gcttgccaac cggcaacgcg cgcagaaccg
     7021 cctgctggtc gcggtcgcct cgctcctggt cctgaccgtc gtactcctac tgttcgccgc
     7081 gctcggctga ccgccgcgtg ccgggcgccg ccgggagggc tgcgcggctt aatcaaactg
     7141 tcatattggt tgcgcaaaat cgcctcgcga gacctgagca caggtcccga gccgccgtgc
     7201 acggcgcttc agctcaccgc gagctcaccg cgccacgcgt ctcgggagga gttccccaac
     7261 caggagaaca aacgatgcga cgcaaccatc agacgcgcgg caccacgctc gcgctcaccg
     7321 ccctcgtggc cgcgctgctc ggcggcgtgc agccggcgcg ggcgcagacc gagatcttcg
     7381 acgacctgct ggagaagctg cgcgacaagg gtgtgctgag cgacgccgag tacgacgcgc
     7441 tcaagcaggc gcgcgacgaa gagcgcctgc agcaacgcgc cgagcgccgc aagcaggcgc
     7501 tcgaggcggc gaccgcggcc gaacacgagg agaaggccaa agccgaagag aagaccacgc
     7561 tcaagggccg cttccgcgac ggtttcacgt tcgagtccgg tgacaagcag cactccatct
     7621 cggtgaccgg ccgtgtccac gcggactacc gttcgttctc cgaggactcg accaacgcca
     7681 actccgccga cacgttcgac gtgcgccgcg cctacatcgg cttgagcgga aagttctaca
     7741 acgactggac gttcgatgtc accgccgacg tggcccagac cagcgcgccg caactcgacg
     7801 tggcctggct caactggggc cgctacaaac cgatccaggc gcgggccgga cagttcaaga
     7861 tgcccttcag cctggaagag ctgaccagtt cgcggttcat cgacttccag gagcgctccc
     7921 tggtgaactc gctggtcccg gccaaggaac gcggtgcgat gctgcacggc gcattcggca
     7981 aggggttctt ctacggcgcg gccctgtcga acggcgcggg aaagaatgcc aacgagggca
     8041 actcgatcga agactcgaag gacatcatcg ggcggctcgg cgtcaacgcc gcggagtggc
     8101 tgggcaacaa ggatatcgtc ttgcacgtgg ccggcgcgta cagcaccggc accatccctg
     8161 gcggcgtgtc gccggtgggc ggaggggtgc gcaccgaagg ccgcggcctg acgttcttca
     8221 acaccgcggc catgggcgcc gccaccgacg acgtcgaccg ctcgcgcacg ggcggtgagg
     8281 tgtcggtcgc ttggggcccg atcaaggcgc agggcgagta cgtcaagtcc aatttcgaat
     8341 ggaccgcggg cggcgttggc ttcgaccagg acatcaaggc ctactacgcc gaggcgatgt
     8401 ggctgataac cggcgagaag tacgccgacg cctatcgcgg cggcgcctat gccgccatca
     8461 agccgaagaa cgcgttcggc agcggcggct ggggggcctg ggaagtcggg gtgcgctaca
     8521 ccgacttcga cgcggaggac tttgccgcca acgccggcct gaccaacaag gccaccgcct
     8581 acaccgtcgg actcaagtgg attccggtga ccaacgtgcg cttctacctg aattacgtgc
     8641 agaccgattt cgacaccccg gtggcggtga gcggcggcgg caccaccgac gacgagaagg
     8701 cgatcacgct gcgcgccgcg gtgtatttct gaaacgctca ggcgtacctc tgagcgccta
     8761 gctggcgtcc tgctgcggcc ctccccccgc agcgggcgcg gagcggccgc acagcctgag
     8821 gctgtgaggc cgttttcttt tgcggttccg gcggggccgt ttgttacaat cgcgcccagc
     8881 ccatggcctt gctcgagatc cgcaacgtca cccgccgctt cggcgacttc atcgcggtgg
     8941 acgaggtcag cctcagcatc gaggccggcg agttcttctg cctgctgggg ccttcgggct
     9001 gcggcaagac cacgctgctg cgcatgatcg ccgggttcga cgcgcccgac ggcggctcca
     9061 tctggctgga tggcagcgac atgaccggca tcccgccgga ggcgcgcaac gtccacaccg
     9121 tgttccagag ctacgctctg tttccgcaca tgagcgtggc cgagaacatc gccttcccgc
     9181 tgaagatggc gcgggtcgat cccggagaga tcgcccggcg cgtgaacgaa gcgctcgagg
     9241 acgtgtcgct cgccggcatg agcggccgct atccgaacga gctttccggc ggccagcgcc
     9301 agcgcgtggc ggtggcgcgc gcgctggtga accggcccag gctgctgctg ctggacgagc
     9361 cgctcgccgc gctcgacgcc aagctcaagg agcgcatgca gctcgagctg atcgcgctgc
     9421 agaaggaggt ggggatcgcc ttcgtgtacg tgacccacga ccagcaggag gcgctcgccc
     9481 tcgcgcaccg catcgcggtg atgaaccacg ggagggtcga gcagctcgac gagccgtcgc
     9541 gcatctacgg ctacccgacc aaccgcttcg tggcggattt catcggcacc tgcaacatcc
     9601 tggaggccga ggtgctgggt ctggacgggc cgcgcatgcg gctgcagatc gcagggctcg
     9661 ggcaggtgga cgcatcgcgc accgaaaacg cccgcgccgg gcaacgcggc gtcctggcgc
     9721 tgcggcccga gcaagtcgcg atcggaacac ggctggtgcc cagcgccgac gagaaccact
     9781 tcgccggccg cgtgttcgat ttcctctacc tcggcgacgt gaccgtgtac gtcgtggaac
     9841 tgcccgacgg cagccgggtc gaggcgatgt tgcccaactc cgcgccgggg cgcgccaagt
     9901 tcttcgagcc cggcgacgcg gtcgagatca actggcgctt cgacgccggc cgctttctcg
     9961 ccgactgagc cgcggaacgt gcaccccggg cgcctgcaca agtggttggt cagcggcccg
    10021 ccgctcgcct atctgctgat cttcttcgcc atccccacgt tgatcatggt ggtggcctcg
    10081 ttccgcgacc ccgggccgta cggcgggttg gcgccgctgt tcaccgggga gcccggcggc
    10141 gccggcgtcg ccctgaccac cgacaactac gcgcgtttct tcaccagct
//
LOCUS       DASPGE010000307         2788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1134067, whole genome shotgun sequence.
ACCESSION   DASPGE010000307 DASPGE010000000
VERSION     DASPGE010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134067"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VD791_09850"
     CDS             complement(<1..892)
                     /locus_tag="VD791_09850"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HXV08321.1"
                     /translation="MKQHIVRIALGLAITLFFIGHAAKYYNVPFITQLDDIIYDARLR
                     LTMPGGVDERIVILDIDEKSLEEVGRWPWPRDRLAELVEKLFGKYQAAIVGFDVVFAE
                     ADYSSGIRVLDRLADGPMKDVPQFRQQYAELRPALDYDALFAQSIKGKPVVFGYYLNS
                     EKDAKRTGAIPEPVLPKGTFRGRNIPFTTWTGYGGNLPAFLKNAAGAGHFNPITDADG
                     VVRRVPLIAELDGAYYEALSLAMVRAILGFPKVEPGYAPDRFLQRGYSGLEWLQVGEL
                     AIPVDETASALIPYRGHQRSF"
     gene            complement(948..1790)
                     /locus_tag="VD791_09855"
     CDS             complement(948..1790)
                     /locus_tag="VD791_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXV08322.1"
                     /translation="MAKLILSMDGLVLKEIPLSKERTTIGRKPHNDIQIDNLAVSGEH
                     AVIVTILNDCFLEDLGSTNGTLVNGNPVKKHFLQNNDVVELGKYRLKFVGEAPAPAAT
                     AEKADFEKTMVLRPSAMKAAAEQAKSMGAGGAEAARAAAAQQVAASASAAGTVDRDAR
                     TPTHVMHTPTPTPAPVAAPAAPAAAAPAPRSGHPLGAIQILSGGNAGKELELAKPLTT
                     LGKPGVQVAVLTRRPQGYFITHVEGANPPTVNGQAIGTAPHALKDHDVVELAGVKMEF
                     FLKG"
     gene            complement(1807..2631)
                     /locus_tag="VD791_09860"
     CDS             complement(1807..2631)
                     /locus_tag="VD791_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HXV08323.1"
                     /translation="MDLTQNLEIATCTDPGMVRSHNEDSVAADAANGLVVLADGMGGY
                     NAGEVASGMATTVITTEMQQLLAKVQPHEIDPQTNKVLAGELVREQVMKANASIYHAA
                     QSQPQYAGMGTTLVVCLFYDNKTLVAHLGDSRLYRMRDGELTQVTRDHSLLQEQIDSG
                     LISAEQAKHAQHKNLVTKALGIDPSVEPEMHEYDTRPGDIYLLCSDGLPDMVEDDDIS
                     MTLEALGANLKLAAQQLVQMANDNGGKDNVSVILVRVLREYPAARGVVGKFLGWLK"
BASE COUNT          382 a          963 c          944 g          499 t
ORIGIN      
        1 cgaagctgcg ctggtgaccg cgatagggga tgagcgcgct cgcggtctcg tccaccggaa
       61 tggcgagctc cccgacctgc agccactcca gcccggagta gccgcgctgc aggaaacggt
      121 ccggggcata gcccggctcc accttgggaa agccgaggat ggcgcgcacc atggcgagcg
      181 acagcgcctc gtagtacgcg ccgtccagct ccgcgatcaa cggcacgcgg cgcaccacgc
      241 cgtccgcatc ggtgatcggg ttgaagtgcc ccgcgcctgc ggcgttcttg aggaacgccg
      301 gcaggttgcc gccgtagccc gtccaggtcg tgaaggggat gttgcgcccg cggaacgttc
      361 ccttgggcag cacgggttcc gggatcgccc cggtgcgctt cgcgtccttc tcgctgttca
      421 ggtagtagcc gaacacgacc ggcttgccct tgatcgactg cgcgaacagc gcatcgtagt
      481 ccagcgccgg tcgcagctcg gcatactgct gccggaactg cggcacgtcc ttcatcggcc
      541 cgtcggccag gcggtcgagg acgcggatcc cggacgaata gtccgcctcg gcgaacacca
      601 cgtcgaagcc gacgatggcg gcctggtact tgccgaacag cttctcgacc agctcggcca
      661 gccggtcgcg gggccagggc cagcgcccga cctcctcgag gctcttctcg tcgatgtcga
      721 ggatgacgat gcgctcgtcg acgccgcccg gcatggtcag ccgcagccgc gcgtcgtaga
      781 tgatgtcgtc gagctgcgtg atgaacggga cgttgtagta cttggcggcg tgcccgatga
      841 agaacagcgt gatggccagc ccgagcgcga tgcggacgat gtgctgtttc atgcaggctc
      901 cgtcgggccg gcggggccgc gtgcggggcg ccgtgtcgcc ccgcggatca tcccttcagg
      961 aagaactcca tcttgacgcc ggccagctcg accacgtcgt ggtccttcag cgcgtgcggc
     1021 gcggtgccga tcgcctggcc gttgaccgtc ggcgggttgg cgccctcgac atgggtgatg
     1081 aagtagccct gcgggcggcg cgtgagcacc gccacctgca cgcccggctt gccgagcgtg
     1141 gtgagcggct tggcgagctc gagctccttg cccgcgttgc cgcccgagag aatctggatc
     1201 gcaccgagcg gatggccgct gcgcggtgcc ggtgccgccg cagccggggc cgcgggggcg
     1261 gcgacgggtg ccggcgtggg cgtgggcgtg tgcatgacgt gggtcggcgt cctcgcgtcg
     1321 cggtccacgg tgcccgcggc ggacgcgctt gcggcgacct gctgcgcggc cgccgcgcgc
     1381 gcggcctccg cgccgcccgc gcccatggac ttcgcctgct cggccgcggc cttcatggcc
     1441 gaaggccgca gcaccatggt cttctcgaag tccgccttct cggcggtggc cgccggcgcc
     1501 ggcgcctcgc cgacgaactt gagccggtac ttgcccagct cgaccacgtc gttgttctgc
     1561 aggaagtgct tcttgaccgg gttgccgttg accagcgtgc cgttggtgct gcccaggtcc
     1621 tcgaggaagc agtcgttcag gatggtgacg atgacggcgt gctcgccgct caccgccagg
     1681 ttgtcgatct ggatgtcgtt gtgcggcttg cggccgatgg tggtgcgctc cttgctcaag
     1741 gggatctcct tgagcaccaa cccgtccatg ctgagtatca gtttcgccat agccgacccc
     1801 tggagcctat tttagccatc ccagaaactt acccaccacg ccgcgcgcag cagggtactc
     1861 ccgcaggacg cgcacgagta taaccgaaac gttgtctttg ccgccgttgt cattggccat
     1921 ctgcacgagc tgctgcgcgg cgagcttgag gttcgcgccg agcgcctcca gcgtcatgct
     1981 gatgtcgtcg tcctcgacca tgtcggggag cccgtccgag cacagcaggt agatgtcgcc
     2041 ggggcgggtg tcgtactcgt gcatctccgg ctccaccgac ggatcgatgc cgagcgcctt
     2101 ggtgaccagg ttcttgtgct gcgcgtgctt cgcctgctcc gcgctgatca gtcccgagtc
     2161 gatctgctcc tgcagcagcg agtggtcgcg cgtcacctgc gtcagctcgc cgtcgcgcat
     2221 gcggtacagc cgcgagtcgc cgaggtgcgc gaccagcgtc ttgttgtcgt agaacaggca
     2281 caccaccagc gtcgtgccca tgcccgcgta ctgcggctgg ctctgcgcgg cgtggtagat
     2341 ggaggcgttc gccttcatca cctgctcgcg caccagctcg cccgccagca ccttgttggt
     2401 ctgcgggtcg atctcgtgcg gctgcacctt ggccagcagc tgctgcatct cggtggtgat
     2461 cacggtggtc gccatgccgc tcgccacctc gcccgcgttg tagccgccca tgccgtcggc
     2521 gagcaccacc aggccgttcg ccgcgtcggc agcgaccgag tcctcgttgt gcgagcgcac
     2581 catgcccggg tcggtgcagg tcgcgatttc gaggttctgg gttagatcca ctcagttatc
     2641 cgccaaaggg cgcgcgcacc gcgaggttcg ccgcggcgaa tgcgacgacc acagcgaggc
     2701 ccagcagcca gtcgcccttc cagaaccccg atttcaaccc cgcccctccc gcttatccct
     2761 gaggcgcatt gttgcagccc tttgcgag
//
LOCUS       DASPGE010000308        11273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1366936, whole genome shotgun sequence.
ACCESSION   DASPGE010000308 DASPGE010000000
VERSION     DASPGE010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11273
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366936"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VD791_09865"
     CDS             <1..654
                     /locus_tag="VD791_09865"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HXV08324.1"
                     /translation="CELARRLGARHPGFEMSVWEIMDETLRRSGMWDAQTNYARGGQD
                     FAYPFEKMHFLDGFDTADRKFHFRADWGRFGGRWEQMPALPDHFDVIDNATPDKPFRL
                     VAAPARTFLNSTFSETPSSLKRERRPTALIHPEDCAALGVSDGERVQLGNERGEIVVH
                     VKPQDGQQRGVVVVEGIWPNRYFENGLGINALTSADPGWPNGGAVFHDTAVWLRRLG"
     gene            complement(670..1848)
                     /locus_tag="VD791_09870"
     CDS             complement(670..1848)
                     /locus_tag="VD791_09870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HXV08325.1"
                     /translation="MTDFDHDVLIAGAGPAGSHAAYLLARAGMRVALLDRARFPRDKV
                     CGGGLSAKTLTLLETAPGPVVHKTFTGAWIAWRGQRAFAKDLGRPVGCTVARSEFDHW
                     LVLRAQAAGAQFHPESAVRGVTQGARCTQIDTERGVFRARIVLAADGVASTLRAQLFG
                     RRAVAVAPAVEALVYCSEERLQRFGERVLFDLGGMPRGYGWIFPKRDHLNAGVYSIFG
                     GTGIRKHLQAFLRCHGLPADERNVCVRGHAIPVRNALHSYQRGSVWLLGDAAGFAEAV
                     YGEGIYFALKSARLAASVVLEAGGSPAPGEYDRRVRTELEPELRWSQRVGRALFSIGP
                     LAFDPLARSRLACDWFAALITGDLSYRACFWRAVGASPAWLLSRRHALRPGSEWHPAL
                     "
     gene            2013..3455
                     /locus_tag="VD791_09875"
     CDS             2013..3455
                     /locus_tag="VD791_09875"
                     /inference="COORDINATES: protein motif:HMM:NF017142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08326.1"
                     /translation="MPCTVVRLFALFVAAAPAAAEARLHRYTVTVDPDISEIRVRACF
                     DGAPPPYLVAESLDASRALEQVQVEGGRRLEPNGAELKLGPLAPDACIAYTAKVDKFQ
                     GRHQRGSGPTRRVGPDLIAEVGVWLWRPPALAQDEDIEVGFALPEGISASAPWRPVRA
                     ADGSVSAYRLGRGPYDWPAAVAFGHFTEREIVVPGARLRVAVLHGSPEVEWDFVREWL
                     TRAATAVTTLYGRFPIDTAQLIVVPNARGNEPVPSAYVVRGGMPSVHFFINQRKPLSA
                     FLTDWSAVHELSHFLLPYVRPEDAWLSEGMASYYQDVLRARAGMIPAAEAWQHMHQSF
                     RRGMQSMPGVTLADATERMYRDGAFMRVYWSGAAIMLLADQRLRSRSGGAQSLDLALA
                     RLRECCLSPEAGWSALELFAQLDRLTGTSVFAELHEQHVRSNRFPDLDECYRLLGLRP
                     DTTGERIELLEVAEQRAVRDAIMTARERVD"
     gene            3540..3881
                     /locus_tag="VD791_09880"
     CDS             3540..3881
                     /locus_tag="VD791_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HXV08327.1"
                     /translation="MPVTLKTILLLSASNVFMTFAWYAHLKDLNHKPWYVAALASWGI
                     ALLEYLLQVPANRTGFTVMTLPQLKILQEAITLSVFVPFAVLYMHQPVKLDYLWAGLC
                     ILGAVYFIFRS"
     gene            3904..5319
                     /locus_tag="VD791_09885"
     CDS             3904..5319
                     /locus_tag="VD791_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXV08328.1"
                     /translation="MNRELCFLTASELARRIRARAVSSEEVMRAHLEQIERVNPKVNA
                     LVTLLPDRALEGARAADRRLRSGDAPGPLHGLPVAHKDLMPTAGIRTTFGSPIYRDHV
                     PDQDGVLVERLRAAGAILIGKTNTPEFGAGSQTFNPVFGATRNPWDLDKTCGGSSGGA
                     AAALASGMLPIADGSDLGGSLRNPASFCNVVGFRPSAGRVPSWPAVNAWFSLAVLGPM
                     ARTVEDTALMLSAIAGPDPRAPLSISEPGSLFARPLGRDFRGVKVAWSADLGGLPVDP
                     RVTEALKSGRAVLESIGLTVEDATPDLREADEVFRVLRAWNFELAYGELLRTHRALMK
                     DTVIWNIEEGAKLTGPQVGRAEVKRSELYQRVRVFMEQYAFLAAPVAQVPPFDVSQPW
                     VKEIDGRPLTTYLDWMKSCYHISATGLPAISVPCGFTPEGLPVGLQLVGAPRDDFGVL
                     QLAYAFQQATGLWKRRPPPAD"
     gene            5367..6227
                     /locus_tag="VD791_09890"
     CDS             5367..6227
                     /locus_tag="VD791_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08329.1"
                     /translation="MADTRTPRRGAAVLALCALLACAAAGAQEPQPAQPAQQPQPPQP
                     PQPQKPAPKRDRVYLTDLEGTWISRDYLERLRASRAPHATGRQAAGIAIKIQREGNSY
                     PIAITNFQRVVLQAIIDVQPEAKPKEYRLAVAQEDRPGISASELTYIPFRGERNADGV
                     FRTLLIAEPTFAKRRFLTYLRLTEPLETLVNRMVIAGQYVDADGNYYEFTESGEAMLP
                     DAAFAYEVSLDPGNANCELLLSHREREPEGTQRIGFAWKRAELLLYRVSGKKAPFKCG
                     TKPFAVLKRQ"
     gene            6348..7790
                     /gene="mgtE"
                     /locus_tag="VD791_09895"
     CDS             6348..7790
                     /gene="mgtE"
                     /locus_tag="VD791_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HXV08330.1"
                     /translation="MSEAKERAAPESVQQHLQEINAILQKQRLVEGLVHKQEMPRHEL
                     VESLVHKQHLAELQKKLDSLHPADIAYILEALPRDQRLTVWDLVKADRDGEILLEVSE
                     PVRESLIAYMEPAELVAAAETLDADELADLAPDLPDDVMQDVFRALPVEEREQLRAAM
                     SYAEGSVGALMDFDLVTIRDDVTLEVVLRYLRRLDELPDHTDQIFVVDRRERLKGVLP
                     FHTLLVNDPERSVAEVMKTDMLALKPADKAAEAAQAFERYDLVTAPVVDERSRLVGRV
                     TVDAVLDYVREAAENERLNRGGLREEEDIFSSVWASARNRWPWLAVNLVTAFAASRVI
                     GVFEGSIERLVALAALMPIVAGIGGNSGNQTITMIVRSLALGQISQPSARALLRKEIV
                     IALLNGLVWGGIIGFFAMLLYRSVPLGLVMTAAMTLNLLVAALFGVFIPMLMHKLGRD
                     PALGSSVLITAITDSGGFFIFLGLATLCLI"
     gene            8028..8984
                     /gene="msrP"
                     /locus_tag="VD791_09900"
     CDS             8028..8984
                     /gene="msrP"
                     /locus_tag="VD791_09900"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HXV08331.1"
                     /translation="MLIRRKPDIPPSEITPRDVYRRRREFLQAAGTFGAAAGLSAAII
                     LAAPRSARAGAKLANVRKSAFSTSEEQTPFKDVTTYNNYYEFGTDKDEPAENAHTLKI
                     RPWTVSVEGEIAKPKVFDIEELLKLAALEERVYRMRCVEGWSMVIPWVGYPLSELIKR
                     VEPTGKAKYVEFVTLADPQQMPGVRMPVLKWPYVEGLRMDEAQHPLTILCLGLYGEVL
                     PNQNGAPVRVVVPWKYGFKSGKSIVKIRLTERQPQTAWNIAQPREYGFYSNVNPEVDH
                     PRWSQAKERRIGEFGKRPTLMFNGYADQVAAMYAGMDLKKNY"
     gene            9092..9733
                     /locus_tag="VD791_09905"
     CDS             9092..9733
                     /locus_tag="VD791_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HXV08332.1"
                     /translation="MSVKSPSQTQIGWIKATVFAACLIPLALLTWRGVSGGLGANPIE
                     YITHSTGWWTLTFLLITLLVTPLRRLTGLNWLLRLRRMLGLFAFFHVCLHFTTYIWLD
                     QFFDWMSIVKDIAKRPFITVGFSAFLLLIPLAATSTNGMVKRLGAQRWQLLHRLVYAI
                     AILGVVHYWWLVKKDITEPVIFAVLLAILLGARLVWRARKPSSVAPVARAQGV"
     gene            complement(9761..11173)
                     /gene="aspA"
                     /locus_tag="VD791_09910"
     CDS             complement(9761..11173)
                     /gene="aspA"
                     /locus_tag="VD791_09910"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HXV08333.1"
                     /translation="MSEKSFRLEHDLLGDRQVPADAYWGIHTLRALQNFPITGTPISA
                     YPRLIDALACVKQAAALANAELGILDTERTHAIAEACKEIRRGELHDQFVVDVIQGGA
                     GTSTNMNANEVIANRGLELLGRRKGEYAVLHPLDHVNLSQSTNDVYPTAINLALWLAI
                     DELVQAMETLRRGFERKVIEFAGVIKMGRTQLQDAVPMTLGQEFSTFAVMLEEDQSRL
                     REAALLIQEINLGATAIGTGINADPRYAALACRYLVDVSGVPVTTAPNLIEATQDAGA
                     FVQLSGVLKRVAVKLSKTCNDLRLLSSGPRAGFGEINLPAVQAGSSIMPGKVNPVIPE
                     VVNQVAFEVIGNDTTVTFAAEAGQLQLNAFEPIIAHSLFKSLTHLRQACLTLAEKCVA
                     GITANRAYLRKTVERSVGLVTALTPHIGYEAASRLAKEAHENGASVYDLVLHKGLLDQ
                     KQLDALLDPDVLTRPGRVVE"
BASE COUNT         1757 a         3888 c         3869 g         1759 t
ORIGIN      
        1 tgcgagcttg ccaggcggct gggcgcgcgc catcccggtt tcgagatgag cgtgtgggag
       61 atcatggacg aaacgctgcg ccgctccggc atgtgggacg cgcagacgaa ttacgcgcgc
      121 ggcggccagg atttcgccta cccgttcgag aagatgcact tcctggacgg cttcgacacc
      181 gcggatcgca agttccactt ccgggcggac tgggggcgtt tcggtggccg ctgggagcag
      241 atgccggcgc tgcccgacca cttcgacgtg atcgacaacg ccaccccgga caagccgttc
      301 cggctggtgg cggcgccggc ccgcaccttc ctcaactcca ccttcagcga aacgcccagc
      361 tcgctcaagc gcgaacggcg ccccacggcg ctgatccacc cggaagattg cgcggcgctg
      421 ggcgtgagcg atggcgagcg cgtgcagctc ggcaacgagc gcggcgagat cgtcgtccac
      481 gtcaagccgc aggacggcca gcagcgcggc gtggtggtgg tggaggggat ctggccgaac
      541 cgctacttcg agaacgggct ggggatcaac gcgctgacca gcgcggaccc cggctggccg
      601 aacggcggcg cggtgttcca cgacaccgcg gtgtggctgc ggcggctggg ctgagcgcgc
      661 gctgcccggt cacaacgccg ggtgccattc gctgccgggg cgcagcgcat ggcgccggga
      721 cagcagccac gcgggcgagg ccccaaccgc gcgccagaag catgcgcggt agctcaggtc
      781 gccggtgatc agcgcggcga accagtcgca cgccagccgg ctgcgggcga gcggatcgaa
      841 cgccagcggt ccgatcgaga acagcgcacg cccgactcgt tgcgaccagc gcagctccgg
      901 ctccagctcc gttcgcaccc ggcggtcgta ctcgccgggg gccggcgatc cgcccgcctc
      961 cagcaccacg cttgccgcca gccgcgcgct cttgagcgcg aagtagatgc cctccccata
     1021 caccgcctcg gcgaagcccg ccgcatcgcc cagcagccaa acggatccac gctggtagct
     1081 gtgcagcgca ttgcgcaccg ggatggcgtg cccgcgcacg cacacgttgc gctcgtcggc
     1141 cggcaggccg tggcagcgga gaaacgcctg cagatgcttg cggatcccgg tgcccccgaa
     1201 gatcgagtac acccccgcat tgaggtgatc gcgcttcggg aagatccagc cgtacccgcg
     1261 cggcatgccg cccaggtcga acagcacccg ctcgccgaac cgctgcaagc gctcctcgga
     1321 gcagtagacc agcgcctcca cggcgggggc gacggcgacg gcgcggcgtc cgaacagttg
     1381 tgcgcgcagc gtgctcgcga cgccgtccgc cgccaggacg atgcgcgccc ggaacacgcc
     1441 gcgctcggtg tcgatctgcg tgcagcgcgc gccctgcgtc accccgcgca ccgcgctctc
     1501 ggggtggaac tgcgcgcccg cggcctgggc ccggagcacc agccagtgat cgaactcgct
     1561 gcgcgccacc gtgcagccca ccggtcggcc caggtctttc gcgaacgcgc gctggccgcg
     1621 ccaggcgatc caggctccgg tgaaggtctt gtgcaccacc ggcccggggg cggtttccag
     1681 cagtgtgagc gtcttggccg acagtccacc gccgcagacc ttgtcgcgcg gaaaacgcgc
     1741 gcgatcgagc aaggccacgc gcatccccgc ccgggccagc agatacgcgg cgtggctgcc
     1801 cgccggtccg gcgcccgcga tcagcacatc gtggtcgaag tccgtcatcc gtatgccctg
     1861 ccggggccgt ggtgtcggcg atgcccgccg ccggcgggtg gctggacgca ccgccgacac
     1921 gccgggcgcc cccctcatcg agccccgaat ccgtagcgtc gcattctccc ttatcctaag
     1981 gcacgttgtt tgcagcgaag cgcgcgcatc ctatgccctg caccgttgtc cgcctgttcg
     2041 cgctgttcgt cgcggccgct cccgcggccg cggaggcgcg cctgcaccgc tataccgtaa
     2101 ccgtcgaccc cgacatcagc gagatccggg tgcgcgcctg cttcgacggt gcaccgccgc
     2161 cgtacctggt ggccgagtcc ctggacgcgt cccgggcgct ggagcaggta caggtggaag
     2221 gcggcaggcg gctcgagccc aacggcgccg agctcaagct cggcccgctg gcgccggacg
     2281 cgtgcatcgc ctacaccgcc aaggtcgaca agttccaggg ccggcaccag cgcggcagcg
     2341 gtccgacccg gcgcgtgggg ccggatctga tcgccgaggt cggggtgtgg ctgtggcgtc
     2401 cgcccgccct ggcccaggac gaggacatcg aggtcggttt cgccctgccg gaaggcatct
     2461 ccgcctcggc gccgtggcgg ccggtgcgcg cggccgacgg cagcgtcagc gcctaccgcc
     2521 tgggtcgtgg gccgtacgac tggccggcag cggtggcgtt cggacacttc accgagcgcg
     2581 agatcgtggt gcccggcgcg cgcctgcggg tggcggtgct gcacgggtcg cccgaggtgg
     2641 aatgggactt cgtgcgcgag tggctgacgc gcgcggccac cgcggtgacc acgttgtacg
     2701 ggcgcttccc gatcgatacc gcgcagctga tcgtggtgcc caacgcgcgc gggaacgagc
     2761 cggtgccctc ggcctacgtg gtgcgcggcg ggatgccctc ggtgcacttc ttcatcaatc
     2821 agcgcaagcc gctgtcggcg ttcctgaccg actggagcgc ggtgcacgag ctgtcgcatt
     2881 ttctgcttcc ctacgtgcgg ccggaggacg cctggctgtc cgagggcatg gcgagctact
     2941 accaggatgt cctgcgcgca cgcgccggca tgatcccggc ggccgaggcc tggcagcaca
     3001 tgcaccagag ctttcgccgg ggcatgcaga gcatgcccgg cgtcacgctg gccgacgcca
     3061 ccgagcgcat gtaccgcgac ggcgccttca tgcgcgtgta ctggagcggc gcggcgatca
     3121 tgctgctcgc cgatcagcgc ctgcgcagcc gcagcggcgg cgcgcagtcg ctcgatctcg
     3181 cgctcgcgcg cctgcgcgaa tgctgtctgt cgcccgaggc cggctggagc gcgctcgagc
     3241 tgttcgcgca gctcgaccgg ttgaccggca cctccgtgtt cgccgagctg cacgaacagc
     3301 acgtgcgttc caaccgcttc ccggacctgg acgagtgcta ccgcctgctc gggctgcggc
     3361 ccgacaccac cggcgagcgc atcgaactgc tcgaagtggc cgagcagcgc gccgtccgcg
     3421 acgccatcat gacggcccgg gagcgggtag actagcgcgc cgtggaacag accggcgcgc
     3481 gccgcgccat ccgcgagcgc cgccgatcgg ctcggcacga acaacaatca ccgccttcca
     3541 tgcccgtcac gctcaagacc atcctgctgc tcagcgcgtc caacgtgttc atgacgttcg
     3601 cctggtatgc gcatctcaag gatctgaacc acaagccctg gtatgtcgcc gccctggcga
     3661 gctggggcat cgcgctgctc gagtacctgc tgcaggtgcc cgccaaccgc accggcttca
     3721 cagtgatgac cctgccgcag ctcaagatcc tgcaggaggc gatcacgctg agcgtgttcg
     3781 tcccgttcgc cgtgctgtac atgcatcagc ccgtgaagct cgactacttg tgggcgggcc
     3841 tctgcatcct gggcgcggtg tacttcatct tccggtcctg agcggacccg gcggagccct
     3901 ccggtgaacc gtgagttgtg cttcctcacc gccagcgagc tggcgcggcg catccgcgcc
     3961 cgcgcggtgt cgagcgaaga ggtgatgcgc gcccatctgg agcagatcga gcgcgtcaac
     4021 ccgaaggtga acgcgctggt gacgctgctg ccggaccgag cgctcgaagg cgcgcgcgcg
     4081 gccgaccggc gattgcggtc cggtgacgcg ccggggccgc tgcacggcct gccggtcgcg
     4141 cacaaggacc tgatgcccac ggcgggcatc cgcaccacat tcgggtcgcc gatctaccgg
     4201 gatcatgtgc ccgatcagga tggcgtgctg gtggagcgcc tgcgcgccgc cggcgccatc
     4261 ctgatcggca agaccaacac gccggagttc ggcgccggtt cgcagacctt caacccggtg
     4321 ttcggcgcca cgcgcaaccc ctgggacctc gacaagacct gcggcggcag cagcggcgga
     4381 gcggccgcgg cgctggccag cggcatgctg cccatcgcgg acggctccga cctgggtggc
     4441 tcgctgcgca acccggcgag tttctgcaac gtcgtcggtt tccggccgtc ggcaggccgt
     4501 gtgccatcgt ggccggcggt caatgcctgg ttcagcctgg ccgtgctcgg tccgatggcg
     4561 cgaaccgtgg aagacacggc gctgatgctg agcgcgatcg ccggtccgga tccgcgcgcg
     4621 ccgctgtcga tcagcgagcc cggcagcctg ttcgcacggc cgctggggcg cgacttccgc
     4681 ggcgtcaagg tggcatggag cgcggacctg ggtggcctgc cggtcgatcc acgcgtgacc
     4741 gaagctctca agagcggccg agcggtgctg gaatcgatcg gcctgaccgt ggaggacgcc
     4801 acccccgacc tgcgcgaggc ggacgaggtg ttccgcgtgc tgcgcgcgtg gaacttcgag
     4861 ctggcctacg gcgagctgct gcgcacccac cgcgcgctga tgaaagacac ggtgatctgg
     4921 aacatcgagg agggcgccaa gctcaccggc ccgcaggtcg ggcgggccga ggtcaagcgc
     4981 agcgagctgt accaacgcgt gcgggtcttt atggaacaat acgccttcct tgctgccccc
     5041 gtggcgcagg tgccgccctt cgacgtgagc cagccgtggg tgaaggaaat cgacggccgg
     5101 ccgctcacca cctatctcga ctggatgaag tcgtgctatc acatcagcgc caccggcttg
     5161 ccggcgatct cggtgccttg cgggttcacc ccggaagggt tgccggtcgg cctgcagctg
     5221 gtgggcgcgc cgcgcgacga tttcggcgtg ctgcagctcg cgtacgcctt tcaacaggcg
     5281 accggcctgt ggaagcggcg tccgccgccg gccgattgag cgacgccctg ccggccgcgc
     5341 gacacgagaa caccaaccaa acagacatgg ccgatactcg aactccgcgg cgcggtgccg
     5401 ccgtgctcgc actgtgtgcg ctcctcgcct gcgcggcagc cggtgcccag gagccgcagc
     5461 cggcgcaacc ggcacagcaa ccgcagccgc cgcagccgcc gcagccgcag aagcccgcgc
     5521 ccaagcggga ccgcgtctat ctcacggacc tggaaggcac ctggatctcg cgcgactacc
     5581 tcgaacgcct gcgcgcctcg cgtgcgccgc acgccacagg tcggcaagcg gcgggcatcg
     5641 ccatcaagat ccagcgcgaa ggaaacagct atccgatcgc gatcaccaac ttccagcgcg
     5701 tggtgctgca ggcgatcatc gacgtgcagc ccgaggccaa gcccaaggaa taccgcctgg
     5761 ccgtggccca ggaggaccgt ccgggtatca gcgccagcga gctcacctac atcccgttcc
     5821 gcggcgagcg caacgccgac ggcgtgttcc gcaccctgtt gatcgccgag cccaccttcg
     5881 ccaagcgccg cttcctgacc tacctgcggc tgaccgaacc cctggagacg ctggtcaatc
     5941 gcatggtgat cgccggacag tacgtggatg ccgacggcaa ctactacgag ttcaccgaga
     6001 gcggggaggc gatgttgccg gatgccgcgt tcgcttacga ggtgtcgctc gatcccggca
     6061 acgcgaactg cgaactgttg ctcagccatc gcgagcgcga gccggagggc acgcagcgca
     6121 tcggcttcgc ctggaaacgc gccgagctgt tgctgtaccg ggtcagcggc aagaaggcgc
     6181 cgttcaagtg cggcaccaaa cccttcgcgg tgttgaagcg gcagtgaacc gaatgcgccc
     6241 ttaaggcgcg atctagaagc aaccctccca cccgcgccgt gctaggcgcg ctggtatccc
     6301 gcggctagaa tcttgcttgt ccggcccgcc cggccgacaa gcctcccatg tccgaagcca
     6361 aggaacgcgc cgcgcccgaa agcgtccagc agcatctgca ggagatcaac gcgattctgc
     6421 agaagcaacg cctggtcgag ggcctggtgc acaagcagga gatgccccgg cacgagctgg
     6481 tggagtcgct cgtccacaag cagcatctcg ccgagctgca gaagaagctc gactcgctgc
     6541 acccggcgga catcgcctac atcctggagg cgctgccgcg cgatcagcgc ctgacggtgt
     6601 gggacctggt caaggccgac cgggatggcg agattctgct cgaggtgtcc gagccggtgc
     6661 gcgagagcct gatcgcgtac atggagccgg ccgagctggt ggcggcagcg gagacgctgg
     6721 acgccgacga gctggccgac ctcgccccgg acttgcccga cgacgtcatg caggacgtgt
     6781 tccgcgcgct gccggtcgag gagcgcgagc agctgcgcgc ggccatgtcc tacgccgagg
     6841 gctcggtggg agcgctgatg gactttgacc tggtgaccat ccgcgacgac gtgacgctcg
     6901 aggtggtgct gcgttacctg cgccggctcg acgagctgcc cgaccacacc gaccagatct
     6961 tcgtggtgga ccggagggag cggctgaaag gcgtgctgcc gttccacacg ctgctggtga
     7021 acgatcccga gcgctccgtg gccgaggtga tgaagaccga catgctggcg ctgaagccgg
     7081 ccgacaaggc ggccgaggcg gcccaggctt tcgagcgcta cgacctggtc accgcgccgg
     7141 tggtggacga gcgcagccgg ctggtgggcc gcgtgaccgt ggacgcggtg ctggactacg
     7201 tgcgcgaagc ggccgagaac gagcgtctca accgcggcgg gctgcgcgag gaggaggaca
     7261 tcttctcgtc ggtgtgggcc agcgcccgca accgctggcc ttggctggcg gtgaacctgg
     7321 tgaccgcttt cgcggcctcg cgcgtgatcg gcgtgttcga gggcagcatc gagcggctgg
     7381 ttgcgctcgc cgcgctcatg ccgatcgtgg ccggtatcgg cggcaactcg ggcaatcaga
     7441 ccatcaccat gatcgtgcgc tcgctcgcgc tgggccagat cagtcagccc agcgcgcgtg
     7501 cgctgctgcg caaggagatc gtcatcgccc tgctcaacgg cctggtgtgg ggcggcatca
     7561 tcgggttttt cgccatgctg ctgtaccgca gcgtgccgtt ggggctggtg atgaccgccg
     7621 ccatgacgct caatctcctg gtggcggcgc tgttcggcgt gttcatcccg atgctgatgc
     7681 acaagctcgg ccgcgacccg gcgctgggct ccagcgtgct gatcaccgcg atcacggaca
     7741 gcggcgggtt cttcatcttc cttggcctgg ccacgctgtg cctgatctga tataccccac
     7801 gccgagcgcg ccctcgaggt gtgagctgaa aaaccactcc ccgcctctca cgaggcgggg
     7861 tggcgccgca ggcgccgggg cggtgtggtg tacggcggaa gagaggattc ctccttggat
     7921 tccgccgcat ccgggcggcc cgctcggtcc gtataaggcc cgggcggtcg tataagacgc
     7981 gcgtaagggc cgtcggccgc atacgacgaa gacgcaaagg agcgaccatg ttgattcgca
     8041 gaaaacccga catcccgccc tcggagatca cgccgcggga cgtgtaccgg cgcaggcgcg
     8101 agttcctgca ggccgccggc acgttcggtg cagccgccgg cctgagcgcg gcgatcatcc
     8161 tggccgcgcc gcgcagtgcg cgcgccggcg cgaagctcgc caacgtgcgc aagagcgcgt
     8221 tcagcaccag cgaggagcag acgcccttca aggacgtcac cacctacaac aactattacg
     8281 agttcggcac cgacaaggac gagccggccg agaacgcgca cacgctgaag attcgcccct
     8341 ggacggtgtc ggtggagggc gagatcgcga aaccgaaggt gttcgacatc gaggagctgc
     8401 tcaaactcgc cgcgctcgaa gagcgtgtct accgcatgcg ttgcgtggaa ggctggtcga
     8461 tggtgatccc gtgggtcggc tacccgctga gcgagctgat caagcgcgtc gagcccaccg
     8521 gcaaggcgaa gtacgtggag ttcgtcaccc tggccgatcc gcagcagatg ccgggcgtga
     8581 ggatgccggt gctcaagtgg ccgtacgtcg aagggttgcg catggacgag gcgcagcatc
     8641 cgctcaccat tctgtgcctc ggcctgtacg gcgaggtgct gccgaaccag aacggcgcgc
     8701 cggtgcgcgt cgtcgtcccc tggaaatacg gcttcaagag cggcaaatcc atcgtcaaga
     8761 tccggttgac cgagcgccaa ccgcagaccg cctggaacat cgcgcagccg cgcgagtacg
     8821 gcttctactc caacgtcaac cccgaggtgg accatccgcg ctggagccag gcgaaagagc
     8881 gccgcatcgg cgagttcggc aaacggccga cgctgatgtt caacggctac gccgaccagg
     8941 tggcggcgat gtatgccggg atggatctga agaagaacta ctgaggtgcc tggcgcggcg
     9001 atcgcggaga gaacgacttt tctttccccg gattgcacgg cgctgcatcc gggctacgcg
     9061 cgccgcgcac acccttcgga gcgggcaatc gatgtcggtc aagtcgccca gtcaaacgca
     9121 gatcggctgg atcaaggcca cggtattcgc ggcctgcctg atcccgctcg cgctgctcac
     9181 ctggcgcggt gtcagcggcg ggctgggcgc caatccgatc gagtacatca cgcattccac
     9241 cggctggtgg acattgacct tcctgctcat cacgctgctg gtgacgccgt tgcgccggtt
     9301 gaccggcctg aactggctgt tgcgcctgcg gcgcatgctg ggcctgttcg cgttctttca
     9361 cgtgtgtctg cacttcacca cgtatatctg gctcgaccag ttcttcgact ggatgagcat
     9421 cgtgaaggac atcgccaagc gacccttcat caccgtaggg ttcagcgcgt tcttgctgct
     9481 gatcccgctt gcggcgacct ccaccaacgg catggtcaag cgcctgggcg cgcagcgctg
     9541 gcagctgctg caccgcctgg tgtacgcgat cgcgatcctg ggggtggtgc actactggtg
     9601 gctggtgaag aaggacatca ccgagccggt gatcttcgcg gtcctgctcg ccatcctgct
     9661 cggcgctcgc ctggtgtggc gcgcgcgcaa gccctcatcc gtggcgccgg tggcgcgcgc
     9721 ccaaggcgtc tagctggcaa atacgggggc gcgcagcgct ctattccacc acccgtcccg
     9781 gtcgggtcag cacatccggg tccagcaggg cgtcgagctg cttttgatcg agcaggccct
     9841 tgtgcagcac cagatcgtag acgctggcgc cgttttcgtg cgcttccttg gccagccgcg
     9901 aggcggcctc gtagccgatg tgcggcgtca gcgcggtcac cagccccacc gaacgctcca
     9961 ccgtcttgcg cagataggcg cggttcgcgg tgatgcccgc cacgcatttc tccgccagcg
    10021 tcaggcatgc ctggcgcagg tgcgtgaggc tcttgaacag gctgtgggcg atgatcggct
    10081 cgaaggcgtt caactgcagc tgtcccgctt cggcggcgaa ggtcaccgtg gtgtcgttgc
    10141 cgatcacctc gaaggcgacc tgattgacca cttccgggat caccgggttc accttcccgg
    10201 gcatgatgct cgagccggcc tgcaccgccg gcaggttgat ctcgccgaaa cccgcgcgcg
    10261 ggccgctgga cagcaggcgc agatcgttgc aggttttcga cagcttgacc gcgacgcgct
    10321 tcaacacgcc ggaaagctgc acgaaggcgc ccgcgtcctg tgtggcctcg atcaggttgg
    10381 gggcggtggt taccggcacc cccgagacgt cgaccaggta acggcaggcc agggccgcgt
    10441 agcgcgggtc ggcgttgatg ccggtgccga tggcggtggc gcccagattg atctcctgga
    10501 tcagcagggc tgcctcgcgc aggcggcttt ggtcctcctc cagcatcacc gcgaaggtgc
    10561 tgaactcctg gcccagagtc atcggcaccg cgtcctggag ctgggtgcgc cccatcttga
    10621 tcacgcccgc gaactcgatc accttgcgct cgaacccgcg ccgcagcgtc tccatggcct
    10681 ggaccagctc gtcgatggcg agccacagcg ccaggttgat ggccgtggga tacacgtcgt
    10741 tggtgctctg gctgaggttg acgtgatcca gcgggtgcag cacggcgtac tcgcctttgc
    10801 gccggccgag cagctccagg ccgcggttgg cgatcacctc gttggcgttc atgttggtgg
    10861 aagtgcccgc tccgccctgg atcacgtcga ccacgaactg gtcgtgcagc tcgccgcggc
    10921 ggatctcctt gcaggcctcc gcgatcgcgt gcgtgcgctc cgtgtccagg attcccagct
    10981 cggcattggc cagcgccgcg gcctgcttca cgcaggcgag cgcatcgatc aggcgcgggt
    11041 aggcactgat cggcgtgccg gtgatgggga agttctgcag cgcacgcagc gtgtggatgc
    11101 cccagtaggc atccgcgggc acttgccggt cgcccagcag atcgtgctcg agccggaagg
    11161 atttctcgct catggtgccc ccagcgggcg cgggacatgc ttcccgcttg gggcagaata
    11221 cgcaactttc gggccgcctg ccccggggat gtccgggttg gcgcggccaa agg
//
LOCUS       DASPGE010000309         6788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1602990, whole genome shotgun sequence.
ACCESSION   DASPGE010000309 DASPGE010000000
VERSION     DASPGE010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602990"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VD791_09915"
     CDS             <1..180
                     /locus_tag="VD791_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08334.1"
                     /translation="AVDPQVASSIEEFKNRDIYKQLTPEVLAAIPDHKLEQAVVDYVH
                     GKIGDNYLCSLVADF"
     gene            248..538
                     /locus_tag="VD791_09920"
     CDS             248..538
                     /locus_tag="VD791_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08335.1"
                     /translation="MAIAATIFALIHLILVAVPVVTSGGSGEAQAFAAAIFDFPIAWL
                     LGLFAAGRGVLYGSSNTLYILIFSIGGTVMYAAVGALLGYAIHRIIRAFRAA"
     gene            661..1131
                     /locus_tag="VD791_09925"
     CDS             661..1131
                     /locus_tag="VD791_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HXV08336.1"
                     /translation="MRSVIVLVISHVVAAFVGFAVGIYALPILTAPPTPSSAELTSQA
                     AQAAYTGQFRRDLEDSDLLHWGEGTVSVGRESITFVGKLAPGPDYKLYLSPEFVETEA
                     DFERLKGRMVRVGDVKTFENFLVPLPESVDPARYNTVIIWCESFSQFITAASYR"
     gene            complement(1202..2563)
                     /locus_tag="VD791_09930"
     CDS             complement(1202..2563)
                     /locus_tag="VD791_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXV08337.1"
                     /translation="MRVLLGCLAVGLAAHASDLTAQSRTPLDRAGGDSFATPPPPPSR
                     PGPVTPSDPGRADAAPRTLTPADRFDFPGFGRPPGSGIRPGDPVLSDAPAHEPGELLV
                     LWPSPAAAKTGAADIAARFKLQPAERIELPHLGRVLTVYRLASTLNAADLKSHLTLAH
                     GDWIVDYNTRYALLGQPRLYAAAKMGLRGPVKPARRVSIGLLDTAVEPIAALRRATIV
                     RQSFLRTGETASASAHGTALAALLVGEDAAAGFQGLAVGCALYAAEVVRRGEGRETTN
                     VALLLRASDWLIGQRVEVINASLGGPPNRLLHELLAALSLRGIPLIAAAGNDGPAASP
                     VYPAAYPDALAVTATDALDRIYAQANHGGYVDLSAPGVDLWVPDGAAGRYVSGTSFAA
                     ALVTGAVALHLAASATPSVSAEATSVEAALCRSARDLGRIGRDPVFGCGLLQLGVEQR
                     ALR"
     gene            complement(2580..3182)
                     /locus_tag="VD791_09935"
     CDS             complement(2580..3182)
                     /locus_tag="VD791_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXV08338.1"
                     /translation="MAELQHDHVSELLPWYANGTLPAAQRSEVDRHVGACERCRAELA
                     WIARLRADVQAATPQPAGDLGLSRFLERIAPESNVLPLRRRERPRWLMPALALAASVL
                     VAQTLVIGVLLHDRQQTLHTLGGSGVARGALLQVTFAPQATEEQIRTLLASVDARIVD
                     GPGALGVYTLSVAPERADATVRALQAAQDLVASVALSPPR"
     gene            complement(3175..3750)
                     /locus_tag="VD791_09940"
     CDS             complement(3175..3750)
                     /locus_tag="VD791_09940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV08339.1"
                     /translation="MAADDDRARAWLAHIRLGDQGAMAEFYRAYAPQVHAFAMRQLGN
                     AAEADDVVVDTMYEVWSTAKRFAGQSLVRTWLFGIARHRLLDRLRKRRPLESVDVDEL
                     AEVLPGDDEGGYDVLARRQRAAHVAHCLETLSDEQRQCMHLVFYEDLPLAEVAQIQGC
                     PENTVKTRLFHARRKMKRCLERRLGREQDDG"
     gene            3844..5175
                     /locus_tag="VD791_09945"
     CDS             3844..5175
                     /locus_tag="VD791_09945"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXV08340.1"
                     /translation="MSDGIRGAGLHRIVIVGGGAGGLELATRLGDSLGRRRRALVTLV
                     DKSRTHLWKPLLHQVAAGSMDLNDHELDYLFQARWHHFQFQLGRMDGLDREHRQIHLA
                     PTYDETGREVIPARSIPYDTLVLAVGSTTNDFGTPGAAQHAISLDNAQQAALFHSRLI
                     NACLRANAQPGALQPGQLDIAIIGAGATGVELAAELHNTLRELIAFGFERIDPEKDIQ
                     LTIIEAAPKILPALPERLSNEVMKLLQKLKVDVLTGERVTEVSARGVRTATGREVPAE
                     LVVWAAGIKAPDLLKDLGGLETNRLNQLVVHQTLQTTRDPDIFAFGDCAACPWPEKKG
                     WIPPRAQSAHQQASHLYRMLPRHLEGRPLQPFQYRDFGSLVSLGAYSTVGSLMGAITR
                     GSVMIEGWFAGIMYRSLYKMHEYALHGFTKVALDTLARILTRRTEPHVKLH"
     gene            5351..6217
                     /locus_tag="VD791_09950"
     CDS             5351..6217
                     /locus_tag="VD791_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08341.1"
                     /translation="MSISLRTSLAAAICSLGLAQPAAALTVRDWLGTYTMNHDGWRGT
                     LTISESKADCASPAWCALVLRYVDADGNARGGRIERMDERRQQMTFTISFPRNRQRFD
                     AYLFSHEPGKMAGVTFWQGKTFGFFAERKPAVAEALRTPESRELHVLEPRRVEEPAVG
                     NGGSASGLPSDAVVSRRVLPSGEVERRLANGVIERISSGKIVRIMPDGTQQVLMMQQV
                     IPVAPPPPPPGSPEHEWLGGHAQQLLDMIVALASSDPAARQNYLATESDARNVYERIA
                     RRTQTIGYLTQP"
     gene            6227..>6788
                     /locus_tag="VD791_09955"
     CDS             6227..>6788
                     /locus_tag="VD791_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08342.1"
                     /translation="MGKSLAALLLLAGVWSSAAWAQDEGGQASEAKAAPSAETRADRW
                     RNVLEGKLPAAESKQQIPGAGAQPMPAPQPPGGADLSDAQTQEAYRGALREYYAYLQK
                     GLQHRQRVFAWQHFSSITIFVVVILLVGAGVYFAAVQFHRGLDQGAAGATGTQFEAGP
                     GGLKVSSPVLGVIILVISLVFFYLYLV"
BASE COUNT         1081 a         2326 c         2295 g         1086 t
ORIGIN      
        1 gcagtggatc cccaagtcgc ctcatctatc gaagagttca agaatcgcga catatacaag
       61 cagctcacgc cggaggttct tgcggcgatc cctgaccaca aactcgagca agcggtggtt
      121 gactacgtcc atggaaagat aggcgacaac tacctctgtt ctctggtggc tgatttttga
      181 tgccggctaa catggcgttg ccgggagcct gaacttcgcg cgatctagaa tgcgctcaaa
      241 atcagcagtg gcaatcgctg cgacgatctt cgcgctcatt cacctcatcc tcgtcgccgt
      301 tccagtggtg acctctggcg gttctggaga ggcgcaggcg ttcgccgcag cgatctttga
      361 cttcccgata gcttggctgc tcggattgtt tgcagctggc agaggggttt tgtatggctc
      421 atcgaacacg ctctacatct tgatcttcag catcggcggc accgtcatgt acgccgccgt
      481 tggtgcgcta ctcggttacg cgatccaccg tatcattcgc gcattccgag ccgcctaagc
      541 ctgagcttgc agcggcgggc agccggctgc ccgggagccc aaaatcgatg aacgatgcgc
      601 ttgtacagcg catcgataag gcgcaccggc agagccgtcg gtgcgcttga gggttctccg
      661 atgagaagtg tgatcgtcct cgtgatctcg catgtggttg ccgctttcgt gggcttcgcc
      721 gtgggcatct acgcgctgcc gatactgacc gctccgccaa cgccgtcgtc cgcggaactg
      781 acgtcgcagg ccgctcaggc cgcctacacg ggtcagttcc gccgcgacct cgaggacagc
      841 gacctgcttc actggggtga aggtaccgtc tcggtcgggc gggaaagcat cacctttgtc
      901 ggcaagctgg cgccgggccc agattacaag ctgtacctct cacccgaatt cgtcgaaacc
      961 gaggcagact tcgagcggct gaaagggcgc atggtgcggg tgggtgatgt caagaccttc
     1021 gagaacttcc tggtcccgtt gcccgagtcg gtggatccgg ctcgctacaa cacggtcatc
     1081 atatggtgcg agtcattctc acagttcatc acggctgcta gctatagata ggggcgcggt
     1141 tcatgaattc gctgccaaac catgggccct taccgggcga cattttcgcc ggcgctcatc
     1201 gctaccgcag cgctcgttgc tctacgccca gctgcaggag tccgcacccg aacaccggat
     1261 cgcgcccgat gcggccgaga tcgcgcgccg aacggcacag agccgcctcc acgctcgtgg
     1321 cctccgcgct cacggaaggc gtggcgctcg ccgccagatg cagcgcgacg gcgccggtta
     1381 ccagagctgc ggcgaacgac gtgccgctca cgtagcgccc ggccgcgccg tccggcaccc
     1441 atagatccac gccgggcgcg ctgagatcga cgtagccgcc atgattggct tgtgcgtaga
     1501 tccggtcgag cgcgtcggtg gcggtcaccg ccagcgcatc ggggtaagcg gcgggataca
     1561 ccgggctggc cgcgggaccg tcgttgcccg cagcggcgat cagcgggatg ccacgtagcg
     1621 acagcgccgc aagcagctcg tgcagcagcc ggttcggcgg gccgcccagg ctggcgttga
     1681 tcacctccac gcgctgtccg atcaaccagt cgctcgcgcg cagcagcagc gcgacgttgg
     1741 tggtctcgcg cccctcaccg cgtcgcacca cctcggcggc gtacagcgcg cacccaaccg
     1801 ccagcccttg gaacccggcg gcggcgtcct cgccgacgag cagcgcggcg agcgccgtgc
     1861 catgcgcgga ggccgacgcc gtctcccccg tgcgcagaaa gctctggcgt acaatggtcg
     1921 cgcggcgcag tgcggcgatc ggttcgaccg ccgtgtccag caacccgatg ctcacccggc
     1981 gcgcgggctt caccgggccg cgcaggccca tcttggcggc ggcgtacagg cgcggctgcc
     2041 ccagcagcgc atagcgcgtg ttgtagtcca cgatccagtc gccgtgcgcg agcgtcagat
     2101 gcgacttgag gtccgcggcg ttcagggttg aagcgagtcg gtacacggtc agcacgcgac
     2161 cgaggtgggg cagctcgatg cgttccgcgg gctgaagttt gaagcgggcg gcgatgtccg
     2221 ctgcgccggt tttcgcggcc gcgggactgg gccacaagac cagcagctcg ccgggctcgt
     2281 gcgcgggtgc gtcggacagg acagggtccc cgggccggat tccggatccc ggcggccggc
     2341 cgaacccggg gaaatcgaag cgatccgccg gcgtcagcgt gcgcggggcg gcgtcggcgc
     2401 gtccgggatc gctcggcgtc accgggcccg gtctgctggg aggcggcgga ggggtggcga
     2461 agctgtcgcc gcccgcgcgg tcgagcggcg tgcgcgactg tgccgtcagg tccgaggcat
     2521 gtgcggccag acccacagcc agacaaccga ggagcacacg catgcgcact ggtcctccct
     2581 caacgcggcg gcgatagcgc gacggatgcg accaggtctt gcgccgcctg cagcgcgcgc
     2641 accgttgcgt cggcccgctc cggcgccacc gagagcgtgt acacgcccag cgctccgggt
     2701 ccgtcgacga tgcgtgcatc gacgctcgcc aacagcgtgc gtatctgctc ctcggtggcc
     2761 tgcggcgcga aggtgacttg gagcagcgcg ccccgggcaa cgccggaacc ccccagcgtg
     2821 tgcagcgtct gctggcgatc gtgcaatagc acgccgatca ccagcgtctg cgccaccagc
     2881 acgctggccg ccagcgcgag cgccggcatc agccagcgcg gccgctcccg ccgccgcagc
     2941 ggcagcacgt tgctctccgg ggcgatgcgc tcgaggaagc ggctcagccc gaggtcgccg
     3001 gccggctgcg gcgttgccgc ctgtacatcg gcacgcaagc gagcgatcca cgccagttcc
     3061 gcccggcagc gctcgcaggc gcccacgtgc cggtccacct cggaccgctg cgccgccggc
     3121 agcgtgccgt tggcatacca cggcagcagc tcgctcacat gatcgtgttg cagttcagcc
     3181 atcatcttgc tcccgcccca gccgtctttc caggcagcgc ttcatcttcc ggcgcgcgtg
     3241 aaacaggcgc gtcttcaccg tgttctccgg gcagccctgg atctgcgcca cctcggcgag
     3301 cggcaggtcc tcgtagaaca ccaggtgcat gcattggcgc tgctcgtcgg acagcgtctc
     3361 caggcagtgc gcgacgtgcg ctgcgcgttg acggcgcgcc agcacgtcgt agccgccttc
     3421 gtcgtcgccg ggcagcacct ccgccagctc gtccacgtcc acgctctcca gcggccggcg
     3481 cttgcgcagc cggtcgagga ggcgatgccg ggcgatgccg aacagccagg tgcgcaccag
     3541 ggactggccg gcgaagcgct tggcggtgga ccacacctcg tacatggtgt ccaccaccac
     3601 gtcgtcggcc tctgccgcgt tgcccagctg gcgcatggcg aaggcgtgca cctgcggcgc
     3661 gtacgcccgg tagaactccg ccatggcgcc ctggtcgccc aggcgaatgt gcgccagcca
     3721 ggcacgcgct cgatcgtcgt ctgctgccac ttcgcgctgt cctctcgccc ctgagtcgca
     3781 ggccgggccg gatcggttca aagaaagtat gctctcgctt cgacgaacaa gcacaacgcg
     3841 acgatgagcg acggcatacg cggtgccggc ctgcaccgga tcgtgatcgt ggggggaggc
     3901 gccggcgggc tggagctcgc cacgcgtctg ggggacagcc tcgggcgcag gcggcgcgcg
     3961 ctggtgacgc tggtggacaa gtcccgcacg catctgtgga agccgctgct gcatcaggtg
     4021 gccgcgggca gcatggatct gaacgaccac gagctggact acctgttcca ggcgcgctgg
     4081 catcacttcc agttccagct cgggcgcatg gacgggctcg accgcgagca ccggcagatc
     4141 catctggcgc cgacctacga cgagaccggg cgcgaggtga tcccggcgcg cagcatccct
     4201 tacgacacgc tggtcctggc ggttggcagc accactaacg acttcggcac gcccggcgcc
     4261 gcgcaacacg cgatctcgct ggacaacgca cagcaggccg cgctgttcca ctcgcggctg
     4321 atcaacgcct gcctgcgtgc caacgcgcag cccggcgcgc tgcaaccggg gcagctcgac
     4381 atcgccatca tcggcgccgg cgccaccggg gtggagctgg ccgcggagct gcacaacacc
     4441 ttgcgcgagc tgatcgcctt cggcttcgag cgcatcgacc ccgagaagga catccagctc
     4501 accatcatcg aggccgcgcc caagatcctg ccggcgctgc ccgagcgcct gtccaacgag
     4561 gtgatgaagc tgctgcaaaa actcaaggtg gacgtgctga ccggcgagcg cgtgaccgag
     4621 gtgtccgcgc gcggcgtgcg caccgccacc gggcgcgagg tgccggccga gctcgtggtg
     4681 tgggccgccg gcatcaaggc cccggactta ctgaaggacc tggggggtct ggagaccaat
     4741 cgcctgaacc agctcgtggt gcaccagacg ctgcagacca cgcgcgatcc ggacatcttc
     4801 gccttcggcg attgcgccgc ctgcccctgg cccgagaaga agggctggat cccgccgcgc
     4861 gcgcaatcgg cccaccagca ggcctcgcac ctttaccgta tgctgccgcg tcatctcgaa
     4921 ggtcggccgc tgcagccgtt ccagtaccgc gacttcggct cgctggtgtc gctgggggcc
     4981 tacagcacgg tgggcagcct gatgggcgcg atcacacgcg gcagcgtcat gatcgagggc
     5041 tggttcgcgg gcatcatgta tcgctcgctc tacaagatgc acgagtatgc gttgcacggc
     5101 ttcaccaagg tggcgctcga caccctcgcg cgcatcctca cgcggcgcac cgagccgcac
     5161 gtgaagctgc attgaagggc gcgtcgcgac aggctcgatc gactcggtcg tcgcatcccg
     5221 ccaccgaacg cacgtcgctc gcgagtcgtt acgcaacgac gagatgacct gggcggtccg
     5281 gcaggggcgg tggtagtctt cccccgacct ctcctcggag gtctcgagct tgcctgtggg
     5341 ggaccaagcc atgtcgattt cgttacgcac atctctggcc gcggcgatct gttcgctggg
     5401 gctggcccag ccggcagcgg cgctgactgt gcgggactgg ctcgggacct acaccatgaa
     5461 ccacgacggc tggcgcggca cgcttaccat ctccgaaagc aaggcggatt gcgcctcgcc
     5521 ggcgtggtgc gcgctggtgc tgcgctacgt cgatgccgat ggaaacgcgc ggggcgggcg
     5581 catcgagcgc atggacgagc ggcgccagca gatgacgttc accatctcgt tcccgagaaa
     5641 ccgccagcgc ttcgatgcct atctgttcag ccatgagccg ggcaagatgg cgggcgtgac
     5701 gttctggcag ggcaagacgt tcgggttctt cgccgagcgc aagccggccg tcgcggaggc
     5761 tctgcgcact cccgagtcgc gcgagttgca tgtcctggaa cccaggcggg tcgaggagcc
     5821 ggcggtcggc aacggcggca gcgcttcggg cctgccgagc gatgcggtcg tcagccggcg
     5881 tgtcctgccc tcgggcgagg tcgagcggcg gctcgccaac ggggtgatcg aacggatcag
     5941 ctcgggcaag atcgtgcgca tcatgcccga cgggacccag caggtgctga tgatgcagca
     6001 ggtgattccg gtcgcgccgc cgccgcctcc tccgggcagc cccgagcacg aatggctggg
     6061 cgggcatgcg cagcagctgc tcgacatgat cgtcgcactc gcctccagcg atcccgccgc
     6121 acgccagaac tacctggcga ccgagagcga cgcgcgcaac gtgtacgagc gaatcgcacg
     6181 gcgcacgcag actatcgggt atctgaccca accgtaggcg gagcacatgg gaaagtcgct
     6241 ggctgcgttg ctgttactgg ccggggtgtg gtcgagcgcc gcctgggcgc aggacgaagg
     6301 cggccaggcg agcgaagcca aggccgcgcc gtccgccgag actcgagcgg accgctggcg
     6361 caacgtcctc gaaggcaaac tgcccgcggc ggagagcaag cagcagatcc ctggtgcagg
     6421 cgctcagccg atgccggcgc cgcagcctcc tggcggcgcg gacctgtcgg atgcgcagac
     6481 gcaggaagcc taccgcggcg cgctgcgcga atactacgcc tacctgcaga agggcctgca
     6541 gcatcgccag cgcgtgttcg cctggcagca cttctcgtcg atcaccatct tcgtcgtggt
     6601 gatcctgctg gtcggcgccg gcgtgtactt cgcggccgtc cagttccatc gcgggctgga
     6661 ccagggggcg gccggcgcaa cgggaaccca gttcgaagcc ggcccgggcg gactgaaggt
     6721 cagctcaccc gtgctcgggg ttatcattct ggtgatctcg ctggtgttct tctacttgta
     6781 cctagtgt
//
LOCUS       DASPGE010000310         4870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1015922, whole genome shotgun sequence.
ACCESSION   DASPGE010000310 DASPGE010000000
VERSION     DASPGE010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015922"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VD791_09960"
     CDS             <1..1109
                     /locus_tag="VD791_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HXV08343.1"
                     /translation="LATTDPQKVPVTVLSRCLQFNLKQIPLAQIRDQLARVLQGESIA
                     FDAPALSLIARAAHGSMRDGLSLLDQAIAHGGGRVDEASVRTMLGAVDQEYLFAILEA
                     LAAADGPALIRLADDMQSRSLSFEAALQDLAALLHRLALAQRWPEALPGDDPDRERLT
                     ALAARFAPEDLQLDYQIALQGRVDLDLAPDEYAGFTMALLRMLAFVPAGSGEGRVQRA
                     GAPASSASPAARPASAGAAASHAISDWRELVTRLKLGGMARMLAQHSEVAGMQGDRLE
                     LRVPEAHKHLLDKPYRDKLQAALEEHLGRRLKLDFVLAPAAGMTPAEQEDRERQTRLQ
                     QAVQAIDGDPFVRDLVENFDARVVDESIRPLEER"
     gene            1128..1454
                     /locus_tag="VD791_09965"
     CDS             1128..1454
                     /locus_tag="VD791_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HXV08344.1"
                     /translation="MMKGQLAGLMKQAQQMQENMRKLQEQLAGVEVEGQSGAGLVKVV
                     MTCKHDVKRVSIDPSLLGDDKDMLEDLVAAAVNDAVRKAEATTQERMAGLTAGMGLPA
                     GFKLPF"
     gene            1518..2114
                     /gene="recR"
                     /locus_tag="VD791_09970"
     CDS             1518..2114
                     /gene="recR"
                     /locus_tag="VD791_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HXV08345.1"
                     /translation="MNSPSSLEELIQALRCLPGVGPKSAQRMAYHLLQHDQQGAMRLT
                     QALERALGVIRHCEKCNTFSEGPMCELCRSERRDGTLLCVVETPADLLMMEQAQCYRG
                     QYFVLMGRLSPLDGIGPREIHLDRLLKRATDGVVTEVVLATNFTVEGEATAHYIGEML
                     RPRGLKVSRIARGVPVGGELEHVDSGTLAQAVLERRPV"
     gene            2281..2961
                     /locus_tag="VD791_09975"
                     /pseudo
     CDS             2281..2961
                     /locus_tag="VD791_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004548140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            complement(3047..3952)
                     /gene="prmB"
                     /locus_tag="VD791_09980"
     CDS             complement(3047..3952)
                     /gene="prmB"
                     /locus_tag="VD791_09980"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HXV08346.1"
                     /translation="MGAPSFADAAGHLRTVRDLLRFAVSRFEDAQLSFGHGSDNAYDE
                     ATYLILHTLHLPLHRLEPFLDAALLPGEIRAVLDVLERRVTQRLPAAYITREAWLGDY
                     RFHVDQRVVVPRSHIAELIEEGLSAWVPDPDAVSDALDLCTGSGCLAILLALAFPAAR
                     VDAVDLSAEALEVARSNVQDYALENRVTLVQSDLFAGLAGRRYQLIVSNPPYLDAEAM
                     RDLPPEHRHEPALGLAAGADGMDAVRRILDQAHRHLLPGGILVVEVGGGRAAFEAAFP
                     RLEPTWLSTRAGDDLVFLLERDRLI"
     gene            complement(3996..>4870)
                     /gene="dapE"
                     /locus_tag="VD791_09985"
     CDS             complement(3996..>4870)
                     /gene="dapE"
                     /locus_tag="VD791_09985"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HXV08347.1"
                     /translation="RDGHLYGRGAADMKTSIAAFVTAVERFTRRHPDHPGSIALLITS
                     DEEGLAVDGTIRVVDVLRARNETLDYCIVGEPSSAEQLGDTIKNGRRGSLNGELRVKG
                     VQGHVAYPQLAKNPVHLAVPALTELAHAIWDEGNEYFPPTTFQISNAQAGTGAANVIP
                     GELRVLFNFRFSTASTVESLKAAVHGVLDRHRLDYQLEWSLSGMPFLTPRGRLVEVAI
                     AAIRDELRVTPQLSTSGGTSDGRFIASICEQLVELGPVNASIHKLNERVALPDVDGLS
                     RVYERMLGALLLEP"
BASE COUNT          798 a         1662 c         1713 g          697 t
ORIGIN      
        1 tcctcgccac caccgacccg cagaaagtgc cagtgacggt gctgtcgcgc tgcctgcagt
       61 tcaacctgaa gcagatcccg ctggcgcaga tccgcgatca gctcgcgcgc gtgctgcagg
      121 gcgagagcat cgccttcgac gcgccggcgt tgagcctgat cgcgcgcgcg gcgcacggca
      181 gcatgcgcga cgggctgagc ctgctcgacc aggccatcgc gcacggcggc ggacgggtgg
      241 acgaggcttc ggtgcgcacc atgctcggcg ccgtcgacca ggaatatctg ttcgcgatcc
      301 tcgaggcgct ggccgccgcg gacgggccgg cgctgatccg gctggcggac gacatgcaga
      361 gccgcagcct gtccttcgaa gcggcgttgc aggatctggc cgcgctgctg catcggctgg
      421 cgcttgcaca gcgctggccc gaggcgctgc ccggggacga tccggatcgg gagcgattga
      481 ccgccctcgc cgcacgcttc gcacccgagg acctccagct cgactaccag atcgcgttgc
      541 agggccgagt cgatctcgac ctggcgcccg acgagtacgc cggcttcacc atggcgctgc
      601 tgcggatgct cgccttcgta cccgcgggca gcggcgaggg cagggtgcag cgcgcgggcg
      661 cgccggcgtc cagcgcttcg ccggcggctc ggccggcgtc ggcgggcgcg gcggcctcac
      721 acgccatctc cgactggcgc gagctggtga cgcgcctcaa gctcggcggt atggcgcgca
      781 tgctcgcgca gcacagcgaa gtcgccggta tgcaggggga tcgcctcgag ttgcgcgtac
      841 ccgaggcgca caagcacctg ctcgacaaac cctaccgcga caagctgcag gccgccttgg
      901 aggaacacct gggccggcgc ctgaagctgg acttcgtgct cgctcctgct gccgggatga
      961 ccccggcaga gcaagaggac cgggaacggc agacgcgcct gcagcaagct gtgcaagcaa
     1021 tcgacggcga tcctttcgtg cgcgacctgg tggagaactt cgatgcgcgc gtggtggacg
     1081 agtcgatccg gccgctggaa gagcgatagc ctgaggagga gcggagcatg atgaagggac
     1141 aactggccgg gctgatgaag caggcccagc agatgcagga gaacatgcgc aaactgcagg
     1201 agcaactcgc cggcgtcgag gtcgagggcc agtccggcgc cggtctggtg aaggtggtaa
     1261 tgacctgcaa gcacgacgtc aagcgcgtga gcatcgaccc cagcctgctg ggcgacgaca
     1321 aggacatgct cgaggacctg gtggccgcgg ccgtcaacga cgcggtgcgc aaggccgagg
     1381 cgaccaccca ggagaggatg gccgggctca ccgccgggat ggggctgccg gccggcttca
     1441 agctgccgtt ctgagcgagc ccgaggcgtg gagcgcgagt gctttcacgt ttcacccctc
     1501 acccttacca taacggcatg aacagcccgt ccagcctcga agagctgatc caggcgttgc
     1561 gttgcctgcc gggagtagga ccgaaatccg cgcagcgcat ggcctatcac ctgctgcagc
     1621 acgaccagca aggcgcgatg cgcctcaccc aggcgctgga gcgcgcgctc ggcgtgattc
     1681 gccactgcga gaagtgcaac accttcagcg agggcccgat gtgcgagctg tgccgctcgg
     1741 agcgccggga cggcacgctg ctgtgcgtgg tcgaaacccc tgccgacttg ttgatgatgg
     1801 agcaggcgca gtgctaccgc gggcagtact tcgtgctgat ggggcgcctt tcgccgctgg
     1861 acggcatcgg gccgcgcgag atccatctgg accggctgct caagcgcgcc accgacggcg
     1921 tggtgaccga agtggtgctc gccaccaact tcacggtgga aggcgaggcc accgcgcact
     1981 acatcggcga gatgctgcgc ccgcgcggac tgaaagtgag ccgcatcgca cgcggcgtgc
     2041 cggtgggcgg agagctggag cacgtggaca gcggcacgct ggcccaggcc gtgcttgagc
     2101 gcaggccggt ctgacccgaa gtaacgtcag tctaaccgag cgtccacgcc cacaatgtcg
     2161 cgcgcatcga agtcctccag ccatcgccgg tccgcccccg ggcgatcgac gcggccggcg
     2221 cgaccccctg cgccggccgc gccccgcttc gaaaccgcag aaccctacag cggaccgcag
     2281 aagatccaca aggtcctggc ccaggccggc ctgggctcgc ggcgcgagat ggaggagctg
     2341 atccgcgccg gcaaggtgaa agtcaacggg gcgctcgccc aggtgggcat gcgcgtgcag
     2401 gcgggcgatg tgatccgcgt gggccggcgc caggtcaccg tgcgcgacga ttccaagctg
     2461 ccgcgcatca tcctctacca caagcccgaa ggcgagatcg tcagtcacga cgatccgcag
     2521 ggccgcccca gcgtgttcga caagctgccg ccgatgcgcc gcggcaaatg gctcgccatc
     2581 gggcggctgg actacaacac ctcggggctg ctgatcttca ccacctccgg cgagctggcc
     2641 aaccggttga tgcacccgcg ctacgaggtg gaccgcgagt atgccgtgcg catcatcggc
     2701 aagctcaccg aggagaagat ccgccagctc acgcgcggcg tgcggctgga ggacggcatg
     2761 gcgcgcttcg aggtcctgga ggaacgcggc gggcgcggcc tcaacaactg gtatcgggtg
     2821 gtgctgcgcg agggccgcaa tcgcatcgtg cgccgcatgt tcgacgcggt cggctacaag
     2881 gtgagccggc tgatgcgggt gcgcttcggc atcgtctcgc tgccgttcgg gctgcgccgc
     2941 ggcggctggc gcgagccgga tgcgcaacag accgccgcga tcctggattg gtccgcgacg
     3001 ctcgccgcgc tcgcccagga gccgctggcg atgcgcgcgc acatcgtcag atcagccggt
     3061 cccgctcgag cagaaagacc agatcgtcgc cggccctggt cgacagccag gtaggttcga
     3121 gccgcgggaa cgccgcctcg aacgccgcgc gcccaccgcc gacctcgacc acaaggatgc
     3181 cacccggcag cagatgccgg tgcgcctggt ccaggatgcg gcgcacggcg tccatgccgt
     3241 cggcgcccgc ggcgagcccc agcgcgggct cgtggcggtg ttccgggggt agatctcgca
     3301 tcgcctccgc gtccaggtac ggcgggttgc tgacgatgag ctggtagcgc cggccggcca
     3361 gcccggcaaa caggtcggac tgcaccagcg tcacccggtt ctccagcgcg tagtcctgga
     3421 cattgctgcg cgcgacttcc agcgcctcgg ccgagagatc cacggcatcc acccgcgcag
     3481 cgggaaacgc cagcgccagc aggatcgcca ggcagcccga gccggtgcac aggtcgagcg
     3541 cgtcgctcac cgcgtccgga tcgggcaccc aggcgctcaa cccttcctcg atcagctcgg
     3601 cgatgtgcga gcgcgggacg acgacgcgtt gatccacgtg gaagcgatag tcgcccagcc
     3661 atgcctcccg cgtgatatag gcggcgggca agcgctgcgt cacgcgccgc tcgagcacgt
     3721 ccagcaccgc gcgtatctcg ccgggcagca gcgcggcgtc gaggaacggc tccagtctgt
     3781 gcaagggcag gtgcagggtg tgcaggatca ggtaggtggc ttcgtcgtag gcgttgtcgc
     3841 tgccgtggcc gaagctcaat tgcgcatcct cgaagcggct gacggcgaag cgcagcaggt
     3901 cgcgcacggt gcgcagatgt cccgccgcgt cggcgaacga aggcgccccc aacaaatacc
     3961 ctcctggggc gccgctcatc gatcgccgcg ggcgctcacg gctcgagcag cagggcgccg
     4021 agcatgcgct cgtacacccg gctcaacccg tccacgtcgg gcagcgcgac gcgttcgttg
     4081 agcttgtgga tggacgcgtt gaccgggccc agctccacca gctgctcgca gatcgaggcg
     4141 atgaagcgcc cgtcggaggt gccccccgac gtggacagct gcggcgtgac gcgcagctcg
     4201 tcgcggatgg cggcgatcgc gacctccacc aggcggccgc gcggcgtgag gaacggcatt
     4261 ccggacaacg accactccag ctgataatcg agccggtgcc ggtcgagcac accgtgcacc
     4321 gcggctttca ggctttccac cgtgctggcc gtagagaagc ggaagttgaa cagcactctg
     4381 agctcgccgg ggatcacgtt ggccgcgccg gtgccggcct gcgcgttgga gatctggaaa
     4441 gtcgtcggcg ggaaatactc gttgccttcg tcccagatgg cgtgcgccag ctcggtcaaa
     4501 gcgggaacgg ccaggtgcac cgggttcttc gccagctgcg gataggccac gtgcccttgc
     4561 acgcccttca cccgcagctc gccgttgagc gagccgcgcc ggccgttctt gatggtgtcg
     4621 ccgagctgct ccgccgagct gggttctccc acgatgcagt agtcgagcgt ctcgttgcgc
     4681 gcgcgcagga catccaccac ccgtatcgtg ccgtccaccg ccaggccttc ctcgtcggag
     4741 gtgatcagca gcgcgatcga gcccgggtgg tcgggatggc gtcgcgtgaa gcgttcgacc
     4801 gccgtgacga aagcggcgat ggaggtcttc atgtcggctg cgccgcggcc atacagatgc
     4861 ccgtcgcgtt
//
LOCUS       DASPGE010000311         3478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_549039, whole genome shotgun sequence.
ACCESSION   DASPGE010000311 DASPGE010000000
VERSION     DASPGE010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549039"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VD791_09990"
     CDS             complement(<1..256)
                     /locus_tag="VD791_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08348.1"
                     /translation="MSRAFVKGDDSDLSGEEVPERPISPHANYVTAEGLAQLRRRYET
                     LLAQHAELKAAGEDFDKPRLTQIDRDLRYFSQRLESAIPID"
     gene            complement(321..1466)
                     /locus_tag="VD791_09995"
     CDS             complement(321..1466)
                     /locus_tag="VD791_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXV08349.1"
                     /translation="MKITDIKVHALQCPLTQPFAFSQGWVNRRCATLVEVLTDAGVSG
                     WGEAFAQGLEPPQIAVAAIQSALQPLLIGADPLDTEVLWHRMYHATRDYGRKGSVVAA
                     ISAIDIALWDIAGKVHNCPVYRLLGGAFRQRVQAYATGFYRISGQGEASRLGEEAIRH
                     YEAGFRAMKVKLGYGVRDDILVMEEVRRTLGQREVTLMVDTNHAYGAAQALQLGRALE
                     QYQLRWYEEPVAPEDLAGYRELRQALATPIAGGENEHTLFGFRDLLGQRCVDIAQPDI
                     ASAGGFTACRHIAAMAHAHGVQVNPHVWGSAIAQAASLQLIAALPAAHHSLFGQEPIF
                     EYDRSSHPFRQQLVREPLESRDGWLEVPSLPGIGVEVDRAVLERYRL"
     gene            complement(1529..1604)
                     /locus_tag="VD791_10000"
     tRNA            complement(1529..1604)
                     /locus_tag="VD791_10000"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1569..1571),aa:Thr,seq:cgt)
     gene            complement(1640..2422)
                     /locus_tag="VD791_10005"
     CDS             complement(1640..2422)
                     /locus_tag="VD791_10005"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXV08350.1"
                     /translation="MSYDNILVETRGKVGLITLNRPKALNALSPELMRELARALAAFE
                     ADAAIGAMVITGNEKAFAAGADIKAMKDWGYMDVYLADYITAEWETVSRCRKPVIAAV
                     AGYALGGGCELAMMCDFILAGDNARFGQPEINLGILPGAGGTQRLPRFVGKSKAMEMV
                     LLGQARMMDAAEAERCGLVSRVVPAAELVEEAVRTAQKIAELSLPIVMLAKESVNRAF
                     ETTLAEGVRFERRLFHSAFATEDQKEGMAAFAEKRKPGFKNR"
     gene            2545..3336
                     /gene="paaG"
                     /locus_tag="VD791_10010"
     CDS             2545..3336
                     /gene="paaG"
                     /locus_tag="VD791_10010"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HXV08351.1"
                     /translation="MPFENILFEIAGGVAAITLNRPDKLNSFTEAMHVELREALQRVR
                     SDAAVRALLITGAGRGFCAGQDLSERVMSGGGAEFDVGATLEENYNPLVRGLRALPIP
                     VLCAVNGVAAGAGCNFALACDIVLAARSARFIEVFSRIGLIPDAGGTYLLPRLIGTAR
                     AMGMALTAEALSAEQAQAWGLIWKCVDDAELMPQARNLAATLAAGATRGFALTKQALY
                     ASSGNSIEQQLALEARLQREAAHTADFREGVQAFLEKRTARFSGK"
BASE COUNT          513 a         1222 c         1188 g          555 t
ORIGIN      
        1 ggtcgatcgg gatggcgctc tccaggcgct gggagaagta gcgcaggtcg cggtcgatct
       61 gcgtgagcct gggcttgtcg aagtcctcgc ccgccgcctt cagctcggca tgctgcgcca
      121 gcagcgtttc gtagcgccgg cgcaattggg ccaggccctc tgcggtcacg tagttggcgt
      181 gcgggctgat cgggcgctcc ggcacttcct cgcccgacag atcgctgtca tcgcccttca
      241 cgaaggcgcg gctcatgatc ttctgtcctc cattctcccg ctgggcgctg cggcgaatgc
      301 aggccgcacc cgatagccga ttatagccgg tagcgctcga gcaccgcgcg gtccacttcc
      361 accccgatcc cgggaaggga aggcacctcg agccagccgt cgcggctctc cagcggctcg
      421 cgcaccagct gctgccggaa cgggtgggag gagcggtcgt actcgaagat cggctcctgg
      481 ccgaacagcg aatgatgcgc cgccggcagt gcggcgatga gctgcaggga cgccgcctgc
      541 gcgatcgccg agccccacac gtgcgggttc acctgcacac cgtgggcgtg cgccatggcg
      601 gcgatgtgcc ggcaggcggt gaaccccccg gccgaggcga tgtcgggctg ggcgatgtcc
      661 acgcagcgct gtcccagcag gtcgcggaag ccgaacagcg tgtgctcgtt ctcgcccccc
      721 gcgatgggcg tggcgagcgc ctggcgcagc tcgcggtagc cggccaggtc ctcgggggcg
      781 accggctcct cgtaccagcg cagctgatac tgctccagcg cgcgcccgag ctgcagcgcc
      841 tgcgccgcgc cgtaggcgtg attggtgtcc accatcaacg tgacctcgcg ctgccccagg
      901 gtgcggcgca cctcctccat caccaggatg tcgtcgcgca cgccgtaacc gagcttcacc
      961 ttcatcgccc gaaagcccgc ttcgtagtgc cggatcgcct cctcgcccag gcggctggcc
     1021 tcgccctgcc cggagatgcg gtagaagccg gtggcgtacg cctgcaccct ctggcggaat
     1081 gcacccccga gcaaccggta gaccgggcag ttgtgcacct tgccggcgat gtcccacagc
     1141 gcgatgtcga tcgcgctgat ggcggccacc accgagcctt tgcgcccgta gtcccgggtg
     1201 gcgtggtaca tgcgatgcca cagcacctcg gtgtcgagcg gatcggcacc gatcagcagc
     1261 ggctgcaggg cggactggat cgccgccacc gcgatctgcg gcggctccag cccctgggcg
     1321 aaggcctcgc cccagccgct gacacccgcg tcggtgagca cctccaccag cgtggcgcag
     1381 cgacggttga cccagccctg ggagaaggcg aacggctgag tcagcgggca ctgcagcgcg
     1441 tgcaccttga tgtcggtgat cttcactgca gcggtctcca gtggcctggc ttggtgcagg
     1501 ttcgcggaac cccgcggggc ttgaggaatg gtgctgctgc tcagactcga actgaggacc
     1561 tatcgattac gaatcgattg ctctaccaac tgagctacag cagcgcccga gactttcatt
     1621 atggggccgt cgaattatat cagcggttct tgaagcccgg cttgcgcttc tcggcgaacg
     1681 ccgccatgcc ttccttctgg tcctcggtgg cgaacgccga gtggaacagc cggcgctcga
     1741 agcgcacgcc ctcggcgagc gtggtctcga aggcgcgatt gaccgactcc ttggccagca
     1801 tcacgatcgg cagcgacagc tcggcgatct tctgcgccgt gcgcaccgct tcctcgacca
     1861 gctccgccgc cggcaccacg cggctcacca gcccgcagcg ttccgcttcc gccgcgtcca
     1921 tcatgcgcgc ctggccgagc agcaccattt ccatcgcttt ggacttgccc acgaagcgcg
     1981 gcaggcgctg ggtgccgccg gcgcccggca ggatgccgag gttgatctcc ggctggccga
     2041 accgggcgtt gtcgccggcc aggatgaagt cgcacatcat ggccagctcg cagccgccgc
     2101 ccagggcgta gccggccacc gccgcgatca ccggcttgcg acagcgcgac accgtctccc
     2161 attcggcggt gatgtagtcg gccaggtaca cgtccatgta accccagtcc ttcatcgcct
     2221 tgatgtcggc gccggcggcg aaggccttct cgttgccggt gatcaccatg gcgccgatgg
     2281 cggcgtccgc ctcgaacgcg gcgagcgcgc gcgccagttc gcgcatcagc tcgggcgaca
     2341 gcgcgttgag cgccttgggg cggttcagcg tgatcagacc gaccttgccg cgggtctcga
     2401 ccagaatgtt gtcgtagctc atggcgttgc tgtcctggcg ggttgcggga ctgaatctat
     2461 aatgccgggg cgcgcgcccg cggcgcttcc atgcacactt ctcccatgca gcactcgtcc
     2521 ctgcacactt cccaaggaac gctcatgccc ttcgaaaaca ttctattcga gatcgccggc
     2581 ggggtggccg cgatcacgct caaccgcccg gacaagctca attccttcac cgaagcgatg
     2641 cacgtggagc tgcgcgaagc gctgcagcgc gtgcgcagcg atgcggcggt gcgggcgctg
     2701 ctcatcaccg gcgcgggacg cggcttctgc gccggtcagg acctgagcga gcgggtgatg
     2761 tcagggggcg gcgcagagtt cgacgtcggc gccacgctgg aggaaaacta caacccgctg
     2821 gtgcgggggc tgcgcgcgct gccgatcccg gtgctgtgcg cggtgaacgg ggtagccgcc
     2881 ggcgccggct gcaacttcgc gctggcctgc gacatcgtgc ttgcggcgcg ctcggcgcgc
     2941 ttcatcgagg tgttcagccg catcgggctg atccccgatg ccggcggcac gtacctgctg
     3001 ccgcggctga tcggcaccgc gcgcgccatg ggcatggcgc tcaccgccga ggcgctgagc
     3061 gcggagcagg cgcaggcctg gggcctgatc tggaaatgcg tcgacgacgc cgagctgatg
     3121 ccgcaggcgc gcaacctcgc cgccacgctc gccgccggcg ccacgcgcgg cttcgcgctc
     3181 accaagcagg cgctgtacgc gtcgtccggc aacagcatcg agcagcagct cgcgctcgag
     3241 gcgcgattgc agcgcgaggc cgcgcacacg gcggattttc gcgaaggcgt gcaggcgttc
     3301 ctggagaagc gcacggcacg cttcagcggg aagtgagcgc ccgcgcggaa gcgcctgtgc
     3361 gggataggtg gcgcgcggcc gcccgcgggg tgggcaagcg cggcggcctc agtcgccggc
     3421 cgagcgcacc cgcaggatga tgtccatgcc ctcggtgacc acgcccgcag gcagcctg
//
LOCUS       DASPGE010000312         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1603666, whole genome shotgun sequence.
ACCESSION   DASPGE010000312 DASPGE010000000
VERSION     DASPGE010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603666"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2193
                     /locus_tag="VD791_10015"
     CDS             <1..>2193
                     /locus_tag="VD791_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08352.1"
                     /translation="EPENTGGSTTPTTPVTGSAGGGDWLRGLLGGGDDPTGFSAHAST
                     VTPKDLDVPNTMSGVPQALIAGAINGPAGHYVKDAGSFAQDLPFGMATDAVNDAIAPY
                     REWDAKAREGAPVAYAIADTVSSAFDPINKVAGVAKLAGAPGSKMRAGVNALTQGGIA
                     MGDSALREYGETGSVEGPDWVSGLVGGTLAAPQSVVAMGAERYAARQSTPQARQLRME
                     AQQQARLRHAGIEPSSREWAGLDPAKQAEILAELEVHASTQGKGRFELSTTKDMNLAA
                     RQAADSAGADKDAAIAALTAAGAEVDPAQIASNVRGNKSQYVPGDPLGDPKRAVLEDM
                     AQGYDDLAAAQPLRPVPSAAPSTPPPTAATPPPLPPSAPPYSPYPMRKPVPPISSWAE
                     MPNAPPWQERSELQPFVEAPASAPPGSAGAPPWQDRVDVQASQAATQPTANPGMRGMG
                     ALPPPPLPAGPQDAISALAEAGQAPSWRDRPDLSPFIAEPSAASLPPSAAAPPWQDRI
                     AAPQSSDPAMSAGMQGLGVPAQQIPRYAPQLPPPPLPAGPLAAAPPQGQIMAPPDTGL
                     YAAGGVPPEVDRMGLPFNQADMIRDTAGKNMKTAASGVVVPTPQNEFDRERYAAIMNA
                     MREGGNATDPALTQQWDLSNQQQGLNLDIAGMGHAAANAKPAPIFNFQDNKLSYMLGG
                     LGGAVGGAVGMSPLGLAAGAGAGIAAGRYLQPRMNSLQANWLSRDTAAM"
BASE COUNT          378 a          796 c          733 g          286 t
ORIGIN      
        1 gaacccgaga acaccggcgg ttccacgacg ccgaccacac ccgtcaccgg aagcgcgggt
       61 ggcggcgact ggttgcgcgg attgctcggc ggcggcgatg atcccaccgg cttcagcgcg
      121 cacgcttcga cggtcacccc caaagacctg gacgttccga acacgatgag cggcgttccc
      181 caggcgctca tcgctggcgc gatcaacggg cccgcgggcc actacgtcaa agacgcgggc
      241 agcttcgcgc aggacctccc gtttgggatg gccacggacg cggtcaacga tgcgattgcg
      301 ccctaccgcg agtgggacgc caaggcgcgc gagggtgcgc ccgtcgccta tgccattgcg
      361 gacacggtga gctccgcgtt cgatccgatc aacaaggtag caggcgtggc caagctcgct
      421 ggcgccccgg gcagcaagat gcgcgctggc gtcaacgcgc tcacgcaagg cggcatcgcc
      481 atgggcgaca gtgcgctgcg cgagtacggt gagacgggca gcgtggaggg cccggactgg
      541 gtcagcgggc tggttggtgg cacgctggcg gcgccgcaat ctgttgtggc catgggcgcc
      601 gagcgttacg cagcgcgtca gtcgacgccg caggcgcgac aactgcggat ggaagctcag
      661 cagcaggctc gcctgcgcca cgctggcatc gagccgagct cgcgcgagtg ggccgggctc
      721 gatcctgcca agcaggctga gatcctcgcc gagttggaag tgcacgcgag cacgcagggc
      781 aaaggccgtt tcgagctcag caccaccaaa gacatgaacc tcgcagcgcg tcaggccgct
      841 gattccgcag gcgcggacaa ggacgcggcc atcgcggcgc tcaccgcagc cggcgccgag
      901 gtagaccccg ctcaaatcgc gagcaacgtg cgcggcaaca aaagccagta cgtgcccgga
      961 gacccgctcg gcgatcccaa gcgcgccgtg ctcgaggaca tggcccaggg gtacgacgac
     1021 ctggccgcgg cccagccttt gcgcccagtc ccctctgcgg caccgagcac cccgccgccc
     1081 acagctgcaa cgcctccgcc gctgcctccg agcgcgccgc cgtacagccc ataccccatg
     1141 cgcaaaccgg tcccgccgat ctcgagttgg gccgagatgc ctaacgcgcc gccctggcaa
     1201 gagcgcagcg agctccaacc gtttgtcgaa gccccggctt cggccccgcc gggctctgcc
     1261 ggagcgccgc cctggcaaga ccgtgtcgac gttcaggcga gccaggccgc aacgcagccc
     1321 accgccaatc ccggcatgcg aggcatgggc gcgctgccgc cgcctccgtt gccggctggc
     1381 ccgcaggacg ccatcagcgc gcttgcggaa gcgggtcagg caccttcgtg gcgcgatcgc
     1441 ccggacctga gtccgttcat cgcggagccc tccgctgcat cgctgccgcc gtcagctgcc
     1501 gcgccgccgt ggcaggatcg catcgctgcc cctcagtcga gcgacccggc gatgagcgca
     1561 ggcatgcagg gactgggcgt ccccgcgcag cagatcccac gctacgcgcc gcagttaccg
     1621 cctccgccgc tgcctgcggg accgctcgcc gcggcaccgc cgcaaggtca gatcatggcg
     1681 ccgcccgaca cggggctcta cgccgccggc ggtgttccgc ccgaggtcga tcgcatgggt
     1741 ctgccgttca accaggcaga catgattcgc gacaccgcgg gcaagaacat gaagacagcc
     1801 gcgtccggcg tcgtggtgcc gacgccgcaa aacgaattcg accgcgagcg ctacgccgcg
     1861 atcatgaacg cgatgcgcga aggcggaaac gccaccgatc ccgcgctcac gcagcagtgg
     1921 gacctcagca atcagcagca aggtctaaac ctcgacatcg cggggatggg ccacgcggct
     1981 gcaaacgcga aacctgcccc gatcttcaac ttccaagaca acaagctgtc ctacatgctc
     2041 ggcggtttgg gtggcgcggt tggaggtgcc gtgggcatgt cgccgcttgg tctcgccgcc
     2101 ggagccggcg ccggcattgc cgcgggtcgc tacctgcagc cacggatgaa ctcactgcaa
     2161 gccaactggc tgtcgcgcga caccgccgcg atg
//
LOCUS       DASPGE010000313        18237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1721358, whole genome shotgun sequence.
ACCESSION   DASPGE010000313 DASPGE010000000
VERSION     DASPGE010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18237
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721358"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VD791_10020"
     CDS             <1..336
                     /locus_tag="VD791_10020"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV08353.1"
                     /translation="VLIQMEYRVDPENRAAFLQAIQDVEPARRRNGASAWRVFRDLED
                     DTRFVERFVIASWAEYVRLRARLTFADQELQERVMRLQRRGVPLRISRLIGVQLEDAL
                     AAPDKAHAG"
     gene            394..687
                     /locus_tag="VD791_10025"
     CDS             394..687
                     /locus_tag="VD791_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08354.1"
                     /translation="MKKTAPSAVLLLCLLALPAAANDDCTAHTADDARGSGEDVRVEA
                     DPPALDGAPSEAGRQRRLGVNLFLCANGLPGCDESLLSPEERARYRVNRPAQQ"
     gene            749..1432
                     /locus_tag="VD791_10030"
     CDS             749..1432
                     /locus_tag="VD791_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV08355.1"
                     /translation="MQQGALIVGAGSGISASLARLLEREGYAVALASRGKAKRAEGVP
                     AVLRLECDAADTASVAAMFARLDAELPPLDVVLYNPSYRTRGPLIELAPEDVRRSLDV
                     TAFGAFLVAQQAARRMLARGSGNILFTGASASVKGYAQSAPFAMGKFALRGLAQSMAR
                     ELAPRNIHVAHLVIDGGVRSAERRETPDQPDSMLDPDAIAQTYLQIIRQPRSAWAWEI
                     ELRPWVEKF"
     gene            1516..1998
                     /locus_tag="VD791_10035"
     CDS             1516..1998
                     /locus_tag="VD791_10035"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXV08356.1"
                     /translation="MSAPSTSHRIVETAVFACLCAGFATMAAIAYSYSLSDELGPGPG
                     FFPFWLGLIGTAISLVLLARSWRGRFLGDGASALWPDRAGALRALALVGGLVLSAVLL
                     SPAGFRVTALLFTAALLLALGVRRPVAIAAFAVVSSFGLFHVFYHWLKVPLPVGPFGL
                     "
     gene            2013..3542
                     /locus_tag="VD791_10040"
     CDS             2013..3542
                     /locus_tag="VD791_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXV08357.1"
                     /translation="MESLTHLLSGFAAALGPAYLAYAFAGCVCGTLIGVLPGLGPAAG
                     TAILIPLTFQLDPTGAIIMLAAIYYGAMYGGTITSVLINVPGEAASVMTCIDGHQMAR
                     QGRGGTALGIAAIGSFLGGLAATCALVMIAPPLAKLALKFGPPEFFALMLLALCLVTG
                     LAGRSLAAALLMTVFGLLISLVGIDPVRGAPRFAFGIPDLYDGFGIVPVVMGLFGLAE
                     ILITAEQPVRDIVRTRLSGLLPSRDEWRRSWGAIWRGTGIGFGLGLIPGIGAIVPTFM
                     AYIAEKRLSKTPERFGRGAIEGVAAPETANNAYANAAMVPFLTLGIPSSPTIAVLMGA
                     FIVNGLTPGPFLFQERPDLVWTVIASFFVGNVILLVLNLPLVGLWAKILHVPFRYLAV
                     GVLLFCLLGAYSLQQSVFDVWVMIAFGLIGYVLRKLDLPLAPLVLGLILGPALEKSLR
                     TSLEMSGGEYAIFFTRPLCLALLLASALVLAGAALQVARTAWRGVIFDRTGGAEGGRE
                     P"
     gene            3551..4537
                     /locus_tag="VD791_10045"
     CDS             3551..4537
                     /locus_tag="VD791_10045"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXV08358.1"
                     /translation="MKRCAPMKNLLMALASLALLSGVAGAQEYPRRPVELMVAYPAGG
                     STDIGARMVAAIAEKLLGQPVVVVNRGGAGGQVGWTELSRRKPDGYFIGFINLPATNT
                     VILDPERKAIFDADAFVPIINQVLDPGVIWVRADSRYKSLKDLIEAARKAPNTIRAAD
                     TGILSDDHLAILMMEEAAPGAIFRIVHLAGGAAQMQEIMAGNVDVAFDNVGSIVPRVR
                     SGEVRALSVMDTQRSKFLPEVPTAAELGYPSVISRSARGIAGPKGLPAPVVKKLVEVF
                     RKAMEDPEHVSRMESTGLALKIMVGAEYERYYKEAHEQAKKYVEWARNRPRK"
     gene            4672..5874
                     /locus_tag="VD791_10050"
     CDS             4672..5874
                     /locus_tag="VD791_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010098583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV08359.1"
                     /translation="MNLRSRQLAFACTAAVVAVIGTGAFAQDRVVKIAGFNGKTGVGR
                     SFGLNAEAAMFAAADTINRSGGVKLGDGARGRIEVTFYDDRCNAEEGISVIRRVASGD
                     AVVAIGPGCSNVAEPLYGILQKKVGDASDSGLQMPVFTDGAIKVGLAKISEWAFRNVP
                     NEQEMYKALFAWLRAEHPDLQTVYGGVEEDFAHSRSTWYAVMKERAKEAGFEVKGETK
                     WLLADTNFATQVRELKAAKADIVALAAHPFTACGVLKEMRRQSVKPKVIVGLTSASSL
                     ETLRGCAAQAEGTYIPTSFAPVTKEASAAAEATARFNGSADLHSMGAWENVFIVKQVI
                     ESEGVLAKPDTVAADRRKIRDGLAKLKETDGLLGKSVRTPDREAIKPFVFVQASAGHW
                     KVAHTPAR"
     gene            5896..6777
                     /locus_tag="VD791_10055"
     CDS             5896..6777
                     /locus_tag="VD791_10055"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV08360.1"
                     /translation="MWALELLQAVFDGLLFGATYAVIGIGFTLIFGVMHKINMAYAAA
                     SLAGAYAALLILVFVQAPALVVFLLAGVAGAALGLVIYVCCFRFMPLTAPLAPLMATI
                     GMLMLIDESIVHATEGMPLPYPALFDDVMLQLGEFTLRGDLLLVFGVSIACMGLMLLL
                     LYRTRLGLATRAVAQQPVAAQLCGVRLTRVNALTFVLTGLFGGVAGAMIGASVGTLSP
                     LLILPVTIKGLIVTVIGGLGSIPGAIVAGLLVGACENLFQFLRGVTERDIYVMLLLFV
                     FLVFRPGGIFAPGRGRD"
     gene            6831..7736
                     /locus_tag="VD791_10060"
     CDS             6831..7736
                     /locus_tag="VD791_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV08361.1"
                     /translation="MDFYFGLAQTLGVHTLLGLSVYIVLLTGQLTLAQAGFFAIGAYV
                     AAMLTVIAGWHIVPALAAGSLATALVACAVAFPALRVRGLMLAVATIAFGEAVRLFFF
                     NFDYQVWKGEVQVGPLGGEGFRQIRFFPENGWSTLEVMLFVWACVALVMVALWWTDRS
                     RAGAVLRAVGEDEVAAQSVGVNLTAVKVSAMTASGAIAGLGGGLYAHYTTHIEHANFT
                     LGLATFAIAYPILGGLSSVFGTLAAVLFIQGFLVEGLRFLGDWRNLLFGTLIVLAMNL
                     RPRGLLDAATTARLKRLLAGPGGAA"
     gene            7733..8497
                     /locus_tag="VD791_10065"
     CDS             7733..8497
                     /locus_tag="VD791_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV08362.1"
                     /translation="MSAALLHLDGVSRRFGGVRAVDAVALTVAKGEILGLIGPNGSGK
                     STIVNLVCGLFPLSGGCIRLEGRDLAAQPPHRRVAAGIARTFQSIRLFGQLTVWQNLW
                     VAQSPPAKRSVAVFAARWLGGQRKAREAIGEALEFAGLAGQRDQLAGNLSFGEQRRLE
                     LARALAARPKLLLLDEPAAGMNATEIAELDERIRRVRDRGITVVLIEHHMELVMSVTD
                     RIAVLNFGRKIAEGSPGEIQAHREVREAYLGAGGEP"
     gene            8494..9264
                     /locus_tag="VD791_10070"
     CDS             8494..9264
                     /locus_tag="VD791_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV08363.1"
                     /translation="MSAAPLLEIADVVTAYGRIEALKGVSLSVAAGTITCLLGPNGAG
                     KTTLMLTVAGILKPKRGAIRFDGASIAGWLPDRVVARGVALVPENRLVFAQMSVRDNL
                     LAGAYLRKDRNAIAADLERMYERFPRLRERAQQHAGTLSGGEQQMLAVARALMSRPRL
                     LLMDEPSLGLAPLVVGDIFRIIAGLKAEGTTVFLVEQNARVALDVADRFYLMEQGRVS
                     FQGTPGELAQDEVIRRAYLGSVRPPHRQPRPPPLAGEG"
     gene            9306..10211
                     /locus_tag="VD791_10075"
     CDS             9306..10211
                     /locus_tag="VD791_10075"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV08364.1"
                     /translation="MIDAVQNTLDGLMIGSSYALLALGFTLTFGVMRRLNLAYGATIM
                     AGVYLGTWLYLEQDAGVAAAAVAAVGGAILASLYVERVCFAPLAERAALASMVSSFAI
                     WMQLEEAAMLALPRHTYPFPALVSGAPLTFGALALRLEHLVMLGSAALIGIGLYLTLR
                     HTRFGRALRAVTDNPLGARLLGIDVGGMRMRAFALAAAVGGIAGFLIAATDQQVTPML
                     GMWATIKGLVAMMLGGLGSLRGAVAGGLLLGVVEAHAQWYLGPQVRDLIAFLLLFACL
                     AVRPAGLLGGARVGGRHPLSGAGRG"
     gene            10204..11112
                     /locus_tag="VD791_10080"
     CDS             10204..11112
                     /locus_tag="VD791_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV08365.1"
                     /translation="MDEFTVGVLSNVGMISFLALSAYLLLVAGEISFGQQGFFAIGAY
                     GAGVGTALWGWPLGVALAWGAAAAAGAAALVGTLTLRLRGMYFAVATLAFAEALRIAF
                     ELIRYQVEVDGEPVGPNGTDGFGGIRHVLEHDIGPVQYLALIYGLLGATLALILWAER
                     TRIGRVLRMIGEDPALAAMQGVDPARAKLAAVTVAGALAGLGGGLYAHLTTYVEPRNF
                     DVMLGVHSLAYGLIGGLGTALGPLLGVAIDIGLLESTRWFSGYRMIVFGGLVAVLLIL
                     RPRGLLDERMVQRLRSAWQFGIHAAR"
     gene            11099..11812
                     /locus_tag="VD791_10085"
     CDS             11099..11812
                     /locus_tag="VD791_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV08366.1"
                     /translation="MLRVDYLGVHFDGVRALDGVGFALAAGEALGLIGPNGSGKTTLF
                     DAICGLVAPDSGRVLFDGEQISGQPAHAIARRGIARTFQGVRLFERMTALENVRCAQP
                     ETGGAVQGGARREAERLLERVGIGQYGAVPAEELPLAARRRLELARVLARRPRLLLLD
                     EPAGAMTQAEVIEMTQLLRSVHAGGCAMIVVEHRIELVAEICPRVLALDCGRVVADGS
                     LDAVLADARVRSAYLGVAG"
     gene            11823..12200
                     /locus_tag="VD791_10090"
     CDS             11823..12200
                     /locus_tag="VD791_10090"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HXV08367.1"
                     /translation="MHPGYTVLLGLLVGAAAAMSCEAAERARAEVRCAPAGVALTYDC
                     LIRLTHAGTGAPLAGARLTVGADMPSMPMAHAVKPVAATPAGEPGLYRARIKLEMTGE
                     WALHLRLSAPFRDRIVSRQRFDE"
     gene            12315..13472
                     /gene="dctP"
                     /locus_tag="VD791_10095"
     CDS             12315..13472
                     /gene="dctP"
                     /locus_tag="VD791_10095"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXV08368.1"
                     /translation="MMPRAKRTERQTPGRAYSKTTGARRKFLEAGAAVGGALLLGAPH
                     IRDARAAAGQVWKIQSTWDAGTTGYKLFEEWCNGFKEKSGGELELKPFPAKAVAADNN
                     ALFDAVRTGVLHAMNPFTLYWAGKIPASVFLSSYPAGPDQPSQWDTLFYGLGMLEATR
                     EIFAKFGLYYVGPIQHDANIIHSKRPVASLDQLKGMKVRLPGGMVAEVFERFGVSTTS
                     MPGSDIYPALEKGTIDAADYVGPAINWDLGFSEVTKYIICMGPPGQTSLYQPVDLMDL
                     TVNAGVWKRLSKKMQVFVEEQVQTYSVHHFVSIQKADLEAMAKFLKSGTTVSRLSAED
                     VVRFRRAAVPVWFNWAKKNADATRIFKLHLDFMLNPYMGYLTPEDLKGLKL"
     gene            13557..14237
                     /locus_tag="VD791_10100"
     CDS             13557..14237
                     /locus_tag="VD791_10100"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HXV08369.1"
                     /translation="MSEHTNFVLPHWLYWGWLLLMPLVLMWFTRGAATAGAPDEGTAV
                     EVLAEEDPALHDPGNRFTRAIDWLSEQSGVFVALWTVNAVCVYFYEVISRYLFDAPTI
                     WAHQASYLMFGMQYLLAGAFALLHGAHVRVDVLYVKLPPRGQIGMDIFTSTFFFIFAL
                     ALLGSCWQFFVNSLDMLERTDETWQVQFYPVKGVMVLGAVLLTLAGVSKLIKDVRLFV
                     RTGGSDSR"
     gene            14234..15772
                     /locus_tag="VD791_10105"
     CDS             14234..15772
                     /locus_tag="VD791_10105"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXV08370.1"
                     /translation="MSTAAAPPAAVRKPRGKLWTWLTWLVTAGVALVVLVEIVNVLFY
                     DPFGDEHFLFTLSGSLEGVGVGTLTALMFGSLAVLLMMGLPMAFVVGGLGVVFIYLVG
                     GQAMINIIPSRIFPLMTNPDLAAIPLFIFMATMLERAGLIEEMFDVVYKWMGGLSGGL
                     AAATILASTILAAMVGVIGAAVVTMGIIALPAMLRRNYDPQLALGSIMAGGTLGILIP
                     PSILAILYAVVAQQSVGKLYLGCLVPGLLLSAMYIAYVLIRTRLRPALGPALPKEERV
                     GMTEKLRLLARLVAPLVLVFLVLGLLFFGIATPVEAAGVGSFGAIVVAVMHKRFSLKS
                     LREASITTARASAMVLWIIFGASIFVGFFILQGGQGFITESILGTGLPAYGILVLMMV
                     LLVFLGMFLDWVGILLLAVPIFVPIVQSLTFDGLLGLPAVPAAEVTLWFGVLFVVNMQ
                     MSFLSPPFGYALFYLRGVAPPEISMGTIFKSSLAFLALQALGLLLCILIPGLATWLPR
                     LAFG"
     gene            complement(15726..16520)
                     /locus_tag="VD791_10110"
     CDS             complement(15726..16520)
                     /locus_tag="VD791_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08371.1"
                     /translation="MRVAFSAFEPGQRIAGSRNVIATGSVARTAKDGDPKIPTRLLFA
                     LCAWIAAFVPATAWAQDPHPAEVCPRGVVGDIPESLRVVKDDSGAILYRHESSPAGVG
                     EEAFFLYIGKKACEVWLRIRIQFPGDRPPGNTRVHIKADDKSFEFAAPRLKQSEDANW
                     RGYWYDELVQPDHLLMLFKVAASARAIVRLEGTDGAAEHVVSDREKQALTVVLGAYHS
                     LGGKISSAPCGVCPGATTAPAVGMRRDARQSVIRTLAAAARWRVRG"
     gene            16745..18163
                     /locus_tag="VD791_10115"
     CDS             16745..18163
                     /locus_tag="VD791_10115"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HXV08372.1"
                     /translation="MKLSPNLRLFALLLLALTLSVGVVVLSERTHRRLGEANQQIAGS
                     TEAQATASELLALVIDAETAQRGLLLTQRPEYLEPYVVALPQIRPKLARLRELTAGAT
                     EVQAHVDRLGELLSERFSELDASLVLYRDRGPKAALELMRTDFGRRSMDRIRGEVDAI
                     QRALRTDLIERHQSWNRDVAFSRLGLAIIAGLNVALLLLVYVQARREILWRERQRRSL
                     VEQRRLLEEQVQERTAQLSELSSNLQNVQEAEKAKLARDIHDELGSILVSARMDVSWA
                     RRRVEPLDPAAAEKLARASSTLDEGVEIKRRIIEDLRPTLLDNLGLAAALNWHVNQAC
                     ERGGLACNLSLPEEDLEVPSPVSIALFRVVQEALTNVLRYAKARNVWLSVEHTETGIS
                     LALRDDGVGIAPRALGDRLSHGIVGMRQRIASLGGTFRIGRVAQGGTAIEVFVPLHGR
                     DAPADDEGAPVGPQQAESIPES"
BASE COUNT         2554 a         6130 c         6431 g         3122 t
ORIGIN      
        1 gtgctgatcc agatggagta ccgcgtggac cccgagaacc gcgccgcctt cctgcaggcg
       61 atccaggacg tcgagccggc caggcgccgc aacggggcga gcgcctggcg cgtgttccgc
      121 gacctggagg acgacacccg cttcgtggag cgcttcgtga tcgcttcctg ggcggagtac
      181 gtacgcctgc gcgcgcgtct gacgttcgcg gatcaagagc tgcaggagcg cgtgatgcgc
      241 ctgcagcgca ggggcgtgcc gctgcggatc tcgcgattga tcggcgtgca gttggaggat
      301 gcgctggccg cgccggacaa ggcgcacgcg ggctgatagc gctgggtccg gttgggtttt
      361 ccggtagcat gccctttcca gaccggagag gacttgaaga agaccgcccc gagcgcggtg
      421 ctgctgctgt gtttgctcgc cttaccggca gcggcgaacg acgactgcac cgcgcatacc
      481 gccgatgatg ctcgcggcag cggcgaggac gtgcgcgtcg aggccgatcc gcccgctttg
      541 gacggcgcgc cttccgaagc cggcaggcag cgccggctcg gagtgaactt gtttctctgc
      601 gccaacgggt taccgggctg tgacgagagc ttgctcagcc cggaggagcg cgcgcggtac
      661 agggtgaacc ggccggcgca acaatagacg tcagcgccgg tccaatggct caaaacggcc
      721 ggaccgggtc gactgtgggg agggaggcat gcaacagggc gccttgatcg tgggcgcggg
      781 ctcgggcatc agcgcatcgc tcgcgcgcct gctcgaacgc gagggctacg ccgtcgccct
      841 ggcctcgcgc ggcaaagcca agcgcgcgga gggcgtgccg gcggtgctgc gcctggagtg
      901 cgacgccgcc gacacggcca gcgtcgcggc gatgttcgcg cgcctggatg cggagctgcc
      961 gccgctcgac gtcgtgctct acaacccgag ctaccgcacg cgcgggccgc tgatcgagct
     1021 ggcgccggag gacgtgcggc gcagcctcga cgtgacggcc ttcggcgcct tcctggtggc
     1081 gcagcaggcc gcgcggcgca tgctcgcgcg cggcagcgga aacatcctgt tcaccggcgc
     1141 ctccgccagc gtcaagggct atgcgcagtc ggcgcccttc gccatgggca agttcgcatt
     1201 gcgcggactg gcgcagagca tggcgcgcga gttggcgccg cgcaacatcc acgtcgcgca
     1261 cctggtgatc gacggcggcg tacgcagcgc cgagcggcgc gaaacacccg atcagcccga
     1321 cagcatgctc gacccggatg cgatcgccca gacgtacctg cagatcatcc gccagccgcg
     1381 cagcgcgtgg gcgtgggaga tcgagttgcg gccgtgggtg gagaagttct agccgccccg
     1441 gccccgacac gctgcgcttt atcgaggaag aatcgcaggc cccgcgcggg gcttcagagg
     1501 aggagcgcgc ccgccgtgag tgcaccgtcg acctcgcacc gcatcgtcga aacagccgtc
     1561 ttcgcctgcc tgtgtgcggg gttcgcgacg atggccgcca tcgcctacag ctactcgctg
     1621 agcgatgagc tggggcccgg acccgggttc ttccccttct ggctggggtt gatcggcacg
     1681 gcgatcagcc tggtcctgct cgcgcggtcg tggcgcggcc gtttcctcgg cgacggagcc
     1741 agcgcgctgt ggccggaccg tgcaggtgcg ctgcgcgccc tggcgctcgt gggcggcctg
     1801 gttctctccg ccgtgttgct gtcgccggcc ggcttccgcg tgacggcgtt gctgttcacc
     1861 gccgcgctct tgctcgcgct gggcgtgcgc cgccccgtgg cgatcgccgc gttcgcggtc
     1921 gtcagcagct tcggcctgtt ccacgtcttc tatcactggc tcaaggtgcc gttgccggtc
     1981 ggcccgttcg ggctctgagg ggaaaatccc gggtggaatc gcttacgcac ctcctgtccg
     2041 gattcgccgc cgcgctcggc ccggcttacc tcgcctatgc gttcgcgggc tgcgtctgcg
     2101 ggacgttgat cggcgtcctg ccggggctgg ggcccgccgc aggcacggcg atcctgattc
     2161 cgctcacctt ccagctcgac ccgaccggcg cgatcatcat gctcgccgcg atctactacg
     2221 gcgccatgta cggcggcacg atcacatcgg tcctgatcaa cgtgcccggc gaggcggcgt
     2281 cggtgatgac ctgcatcgac gggcaccaga tggcgaggca aggccgcgga ggcaccgcgc
     2341 tcggcatcgc cgcgatcggt tcgttcctcg gcggactggc ggcgacatgc gcgctggtga
     2401 tgatcgcacc gccgctcgcc aagctcgcgc tcaagttcgg cccgccggag ttcttcgcgc
     2461 tgatgctgct cgcgctctgc ctggtgaccg gcctggccgg gcgctcgctc gcggcggcgc
     2521 tgctgatgac cgtgttcggt ctgctgatct cgctggtcgg catcgacccg gtgcgcggtg
     2581 caccgcgctt cgcgttcggg attcccgacc tgtacgacgg cttcggcatc gttcccgtgg
     2641 tgatgggtct gttcggcctg gcggagatcc tgatcaccgc cgaacagccg gtgcgcgaca
     2701 tcgtccgcac ccgcttgagc gggcttctgc ccagccgcga cgagtggcgg cgatcctggg
     2761 gcgcgatctg gcgcggcacc gggatcggct tcgggctcgg cctgatcccc ggcatcgggg
     2821 cgatcgtgcc gaccttcatg gcctacatcg ccgagaagcg cctgtccaag acgccggagc
     2881 ggttcggccg gggtgcgatc gagggggtgg ccgcgcccga gacggcgaac aacgcctacg
     2941 cgaacgcggc gatggtgccg ttcctcacgc ttggcatccc cagctcgccg accatcgccg
     3001 tgctgatggg tgcgttcatc gtcaacggcc tcacgccggg gccgttcctg ttccaggagc
     3061 gccccgacct ggtgtggacc gtgatcgcca gcttcttcgt cggcaacgtg atcctgctcg
     3121 tgctcaatct gccgctggtc ggcctgtggg ccaagatcct gcacgtgccg ttcaggtatc
     3181 tggcggtcgg ggtgctgctg ttctgcctgc tgggagccta cagcctgcag cagagcgtgt
     3241 tcgacgtctg ggtgatgatc gcgttcgggc tgatcggcta cgtgctgcgc aagctcgatt
     3301 tgccgctcgc gccgctggtg ctcggcctga tcctcggccc cgccctggag aagtcgctgc
     3361 gcacctcgct ggagatgtcg ggcggcgagt acgcgatctt cttcacgcgg ccgttgtgcc
     3421 tcgcgctgtt gctcgcttcg gccctggtgc tcgccggcgc ggcgctgcag gtggcgcgaa
     3481 cggcgtggcg cggcgtaatc ttcgatcgaa caggtggagc ggaaggaggt cgcgaaccat
     3541 gagaagcccg atgaagcgct gcgcgccgat gaagaacttg ctgatggcgc tggcgtcgct
     3601 cgcgctgctg tccggcgtgg cgggcgcgca ggaatatccc aggcgcccgg tggagctgat
     3661 ggtcgcgtac ccagccggcg gcagcaccga catcggcgcg cggatggtcg cggccatcgc
     3721 cgagaagctg ctcggccagc cggtcgtggt ggtcaaccgg ggaggcgcgg gcggacaggt
     3781 gggctggacc gagctgtcgc ggcgcaaacc ggacggctat ttcatcggct tcatcaatct
     3841 gccggccacc aacacggtga tactcgatcc cgagcgcaag gcgatcttcg atgcggacgc
     3901 cttcgtcccg atcatcaacc aggtgctcga tccgggcgtg atctgggtgc gtgcggacag
     3961 ccgctacaag tcgctcaagg atctgataga ggcggcgcgc aaggcgccga acacgatccg
     4021 cgcggccgac accggcatcc tgagcgacga tcacctggcg atcctcatga tggaagaggc
     4081 cgcgccgggc gcgatcttcc gcatcgtgca cctggccggc ggtgcggcgc agatgcagga
     4141 gatcatggcc ggcaacgtcg acgtcgcatt cgacaacgtc ggcagcatcg ttccgcgcgt
     4201 gcgcagcggc gaagtgcgcg cgctctcggt gatggacacc cagcgctcga agttcctgcc
     4261 cgaggtgccg accgccgcgg agctgggcta tcccagcgtc atctcgcgct cggcgcgcgg
     4321 catcgccggg ccgaaaggcc tgccggcccc ggtcgtcaag aagctcgtcg aggtgttcag
     4381 gaaggcgatg gaggaccccg aacacgtgag ccggatggag agcaccgggc tcgcgctgaa
     4441 gatcatggtc ggcgcggagt acgagcgcta ctacaaagag gcgcacgagc aggcgaagaa
     4501 gtacgtcgag tgggcgcgga accggccgcg caagtagccg caccagaccg gatcggagtt
     4561 cctcgacgct gaccccagtt ccttgacgct ggccaggccc gcgcctagac tggttcgcaa
     4621 acaccatcag ccgcgctgaa gccggcggat tccactccgg gaggacctgc catgaatctg
     4681 cgctcgagac agctcgcgtt cgcttgcaca gccgccgtgg tggccgtcat cggcaccggc
     4741 gcgttcgccc aggaccgggt ggtgaaaatc gccggtttca acggcaagac cggggtgggc
     4801 cgctctttcg gcctgaacgc cgaggccgcc atgttcgcgg ccgccgacac catcaaccgc
     4861 agcggcggcg tcaagctggg cgacggcgcc cgcggccgca tcgaggtcac gttctacgac
     4921 gaccgctgca acgccgagga aggcatctcg gtgatccggc gcgtggcctc gggcgatgcg
     4981 gtggtggcga tcggcccggg ttgctccaac gtagccgagc cgctgtacgg catcctgcag
     5041 aagaaagtcg gcgacgcctc cgacagcggc ctgcagatgc cggtgttcac cgacggcgcg
     5101 atcaaggtgg gcctcgccaa gatctccgaa tgggcgttcc gcaacgtccc caacgagcag
     5161 gagatgtaca aggcgctgtt cgcctggctg cgcgccgagc atcccgatct gcagaccgtc
     5221 tacggcgggg tggaagagga cttcgcgcac tcgcgctcca cctggtacgc ggtgatgaag
     5281 gaacgcgcca aggaggcggg attcgaagtc aagggcgaga ccaagtggct gctcgccgac
     5341 accaacttcg ccacccaggt gcgcgagctg aaggcggcca aggccgacat cgtcgcgctg
     5401 gccgcgcacc cgttcaccgc ctgcggcgtg ctcaaagaga tgcggcgcca gagcgtgaag
     5461 cccaaagtga tcgtggggct caccagcgcc tcgagcctgg agacgctgcg cgggtgcgcg
     5521 gcgcaggccg agggcacgta catcccgacc agctttgccc cggtaaccaa ggaagcctca
     5581 gcggcggccg aggccaccgc caggttcaac ggcagcgccg acctgcacag catgggcgcc
     5641 tgggagaacg tgttcatcgt caagcaggtg atcgaatccg aaggcgtgct cgccaagccg
     5701 gacacggtcg cggccgaccg gcgcaagatc cgcgacggcc tggccaagct caaggagacc
     5761 gacgggctgc tgggcaagtc ggtgcgcacg ccggaccgcg aggcgatcaa gccgttcgtg
     5821 tttgtgcagg cgagcgccgg ccactggaag gtcgcccaca cgccggcccg ctgaagcgct
     5881 gcgcaccgcc gcgccatgtg ggcgctcgag ctgctgcagg cggtcttcga cggcctgctg
     5941 ttcggcgcga cctacgccgt catcggcatc ggcttcaccc tgatcttcgg ggtgatgcac
     6001 aagatcaaca tggcgtacgc cgcggcgtcg ctggccgggg cgtacgccgc gttgctgatc
     6061 ctggtatttg tccaagcgcc tgcgctggtg gtgttcctcc tggctggcgt ggccggcgcc
     6121 gccctcggcc tggtgatcta cgtctgctgt ttccgcttca tgccgctcac cgcgccgctg
     6181 gcgccgctga tggcgaccat cggcatgctg atgctgatcg acgagagcat cgtgcacgcg
     6241 accgagggca tgccgctccc ctatccggcg ctgttcgacg acgtgatgct gcagctgggc
     6301 gagttcacgc tgcgcggcga tctgctgctg gtgttcggcg tcagcatcgc ctgcatgggg
     6361 ctgatgctcc tgctgctgta tcggacgcgc ctgggcctgg ccacgcgcgc cgtggcccag
     6421 cagccggtcg cggcccagct gtgcggcgtg cggctcaccc gcgtcaacgc cctcaccttc
     6481 gtgctcaccg ggctgttcgg cggggtcgcc ggagccatga tcggcgcgtc cgtgggcacg
     6541 ctgtcgccgc tgctcatcct gccggtgacc atcaagggcc tcatcgtcac cgtgatcggc
     6601 gggctgggca gcatccccgg agcgatcgtc gccgggctgc tggtgggcgc ctgcgagaat
     6661 ctgttccagt tcctgcgcgg ggtcaccgag cgcgacatct acgtgatgct gctgctgttc
     6721 gtgttcctgg tgttccgtcc gggcggcatc ttcgccccgg ggcgtgggcg cgattgagcc
     6781 gttgatttcc acttcgggga cttccacttc ggggacttcg acttcgggcc atggacttct
     6841 acttcggact ggcgcagacg ctgggcgtgc acacgctgct cgggctgtcg gtctacatcg
     6901 tgctgctcac cggccagctg accctggcgc aagccggctt cttcgccatc ggcgcctatg
     6961 tggccgccat gctcacggtg atcgccggct ggcacatcgt gccggcgctc gccgccggca
     7021 gcctcgccac cgccctcgtc gcctgcgcgg tcgcgttccc cgcgctgcgc gtgcgcggcc
     7081 tgatgctggc ggtggcgacc atcgctttcg gcgaggcggt gcgcctgttc ttcttcaatt
     7141 tcgactacca ggtgtggaag ggcgaggtcc aggtcgggcc gctcggcggc gagggcttcc
     7201 gccagatccg cttcttcccc gagaacggct ggtccacgct cgaggtgatg ctgttcgtct
     7261 gggcgtgcgt cgccctggtg atggtcgcgc tgtggtggac cgaccgctcg cgcgcgggag
     7321 cggtgctgcg cgcggtgggc gaagacgagg tggcggcgca gagcgtgggg gtgaacctca
     7381 ccgccgtgaa ggtgagcgcg atgaccgcct ccggcgccat cgccggcctg ggcggcgggc
     7441 tgtatgcgca ctacacgacg cacatcgagc acgccaactt cacgctcggg ctggccacct
     7501 tcgccatcgc ctatccgatc ctgggcgggc tgtccagtgt gttcggcacg cttgcggcgg
     7561 tgctgttcat ccagggcttc ctggtggagg gcctgcgctt cctgggcgac tggcgcaacc
     7621 tgctgttcgg gacgctgatc gtgctcgcca tgaacttgcg cccgcgcggg ctgctcgatg
     7681 ccgccaccac cgcgcggctc aagcgcctgc tcgccggccc gggcggcgcg gcatgagcgc
     7741 tgcgctgctg cacctcgacg gcgtatcgcg ccgcttcggc ggggtacggg cagtcgacgc
     7801 ggtggctctc acggtagcga agggcgagat cctcggcctg atcggaccta acggctccgg
     7861 caagagcacc atcgtcaacc tggtgtgcgg gctgtttccc ctgagcggcg ggtgcatccg
     7921 cttggagggc cgcgatctcg cggcgcagcc gccgcatcgc cgcgtcgctg ccggcatcgc
     7981 gcgcacgttc cagagcattc gcctgttcgg gcagctcacg gtgtggcaga acctgtgggt
     8041 ggcgcagagc ccgcccgcga agcgctccgt ggccgtgttc gcggcgcgct ggctcggcgg
     8101 ccagcgcaag gcgcgcgagg cgatcggcga ggcgctcgag ttcgccggtc tggccggcca
     8161 gcgcgatcag ctcgccggca acctgtcctt cggcgagcag cgccggctcg agctggcccg
     8221 ggcgctcgca gcgcgtccga agctgctgct gctcgacgag ccggcggccg gcatgaacgc
     8281 caccgagatc gccgagctcg acgagcgcat ccgcagggtc cgcgaccggg gcatcacggt
     8341 ggtgctgatc gagcatcaca tggagctggt gatgtcggtc accgaccgca tcgccgtgct
     8401 caacttcggc cgcaagatcg cggagggcag ccccggcgag atccaggcgc accgcgaggt
     8461 gcgcgaggcc tacctgggcg cgggaggcga accgtgagcg cggccccgct gctcgagatc
     8521 gccgatgtgg tcacggccta cggccggatc gaggcgctga agggcgtctc gctgagcgtt
     8581 gcggcgggca ctatcacctg cctgctgggg cccaatggcg ccggcaagac cacgctgatg
     8641 ctgaccgtcg ccggcatcct caagccgaag cgcggggcga tccgcttcga cggagcctcc
     8701 atcgcgggct ggctgccgga ccgggtagtc gcgcgcggcg tggcgctggt gcccgagaac
     8761 cggttggtgt tcgcgcagat gagcgtgcgc gacaatctgc tggcgggtgc ctacctgcgc
     8821 aaggaccgca acgcgatcgc cgccgatctg gagcgcatgt acgagcgctt ccctcggctg
     8881 cgggagcgcg cgcagcagca tgccggcacc ttgtcgggcg gcgagcagca gatgctcgcc
     8941 gtggcgcgag cactgatgtc gcggccgcgg ctgctgctga tggacgaacc ctcgctcggc
     9001 ctcgcgccac tggtggtcgg cgacatcttc cgcatcatcg ccggcctgaa ggccgagggc
     9061 accacggtgt tcctggtcga gcagaatgcg cgcgtggcgc tcgacgtggc ggaccgcttc
     9121 tacctcatgg agcaaggccg ggtgagcttc cagggcacgc ccggggagct ggcgcaggac
     9181 gaggtcatcc gccgcgcgta tctcggctcc gtgcgcccac cgcaccgtca accccgccct
     9241 cccccgctcg cgggggaggg ttagggcggc ggcttgcggg ggagcgctgg agtggacgca
     9301 ggggcatgat cgacgcggtc cagaacacgc tggacggact gatgatcggt tcgtcctacg
     9361 ccttgctcgc gctcggcttt accctcacct tcggcgtgat gcgccggctc aacctcgcgt
     9421 acggcgcgac catcatggcc ggcgtctatc tgggcacctg gctgtacctc gagcaagatg
     9481 ccggcgtggc ggccgccgcg gttgctgcgg tgggcggggc gatcctcgcc agcctgtatg
     9541 tcgagcgcgt gtgcttcgca cccctggccg agcgcgcggc cctggcttcc atggtgtcga
     9601 gcttcgccat ctggatgcag ctcgaggagg cggcgatgct ggcgctgccg cgccacacct
     9661 atccgttccc tgcgctcgtg agcggcgcgc cgctcacgtt cggcgcgctg gcgctgcgcc
     9721 tcgagcacct ggtgatgctc ggcagcgcgg cgctcatagg gatcgggctg tacttgacgc
     9781 tgcggcacac gcgcttcggg cgcgcgctgc gcgccgtcac cgacaacccc ctcggggccc
     9841 ggctgctcgg catcgacgtg ggcggcatgc gcatgcgtgc gttcgcgctg gccgccgccg
     9901 tgggcggtat tgcgggcttt ctgatcgccg cgaccgatca gcaggtgacg ccgatgctcg
     9961 gcatgtgggc cacgatcaag ggcctggtgg cgatgatgct gggcggcctc ggctcgctgc
    10021 gcggggccgt ggcgggcggg ctgctgctgg gtgtggtcga ggcgcatgcg cagtggtacc
    10081 tcggacctca ggttcgcgac ctgatcgcgt tcctgctgct gttcgcctgt ctggcggtgc
    10141 gccccgccgg gttgctcggc ggcgcgcgcg tcggcggcag gcatccgctg tcgggagccg
    10201 ggcgtggatg agttcaccgt cggcgtattg tccaacgtcg gcatgatctc ctttctggcg
    10261 ctgtcggcgt acctgctgct ggtggcgggc gagatctcgt tcgggcagca agggtttttc
    10321 gcgatcggtg catacggcgc cggcgttgga accgcgctgt gggggtggcc gctcggcgtg
    10381 gcgctcgctt ggggggcggc ggcggccgca ggcgccgctg cgctggtcgg cacgctgacg
    10441 ctgcgcctgc gcggaatgta cttcgcggtg gccacgctcg ctttcgcgga ggcgctgcgc
    10501 atcgccttcg agctgatccg ctaccaggtc gaggtcgacg gcgagccggt gggcccgaac
    10561 ggcaccgacg gctttggcgg gatccgccac gtgctcgagc acgacatcgg tccggtgcaa
    10621 tatctggcgc tgatctatgg gctgctcggc gccacgctgg cgctcatcct gtgggcggag
    10681 cgcacgcgca tcggccgcgt cctgcgcatg atcggcgagg acccggccct ggcggcgatg
    10741 cagggcgtcg acccggcgcg cgcgaagctc gctgccgtca cggtggccgg ggctctcgcc
    10801 ggcctgggcg gagggctgta cgcgcacctc accacctatg tcgagccgcg caacttcgac
    10861 gtcatgctgg gcgtgcacag cctggcctac gggctgatcg gcggcctggg caccgcgctc
    10921 ggtccgctgc tgggggtggc gatcgacatc gggctgctcg agtccacgcg ctggttctcg
    10981 ggctaccgca tgatcgtgtt cggcgggctg gtggcggttc tgctcatcct gcggccacgc
    11041 ggattgctcg acgagcgcat ggtgcaacgg ttgcgctcgg cctggcagtt cggcatccat
    11101 gctgcgcgtt gactacctcg gcgtgcattt cgacggcgtg cgggccctgg acggcgtcgg
    11161 cttcgcgctc gccgcaggcg aggcgctcgg actgatcggg ccgaacggct cgggcaagac
    11221 cacgctgttc gacgccatct gcggcctggt tgcgcccgac tccggccgcg tgctgttcga
    11281 cggcgaacag atcagcgggc aacccgcgca cgcgatcgca cggcgcggca tcgcgcgcac
    11341 cttccagggc gtgcgcctgt tcgagcgcat gaccgcgctg gagaacgtgc gctgcgcgca
    11401 acctgaaacc ggcggcgccg tgcaaggtgg cgcgcgccgc gaagccgagc ggctgctcga
    11461 acgcgtggga atcgggcagt acggcgccgt gccggcggag gaactgccgc tcgcggcgcg
    11521 ccggcgcctg gagctcgccc gggtgttggc gcgccgcccg cggctgctgt tgctcgacga
    11581 gccggcgggt gcgatgaccc aggccgaggt gatcgagatg acgcagttgc tgcgctcggt
    11641 gcacgcgggt ggatgcgcga tgatcgtggt cgagcaccgc atcgagctgg tggccgagat
    11701 ctgcccgcgc gtgctggccc tcgactgcgg gcgcgtggtg gccgacggca gtctcgacgc
    11761 ggtgctcgcc gatgcgcgcg tgcgctcggc ctacctcggc gtcgccggct gaggccgcta
    11821 ccttgcatcc gggctacacg gttctgctcg gcctcctcgt cggcgccgca gcggcgatgt
    11881 cttgcgaggc agccgagcgc gcgcgcgccg aggtgcgctg cgcaccggct ggggttgcgc
    11941 tcacctacga ctgcctgatc cgcctcaccc atgccggcac cggcgccccg ctcgcgggcg
    12001 cgcgtctcac ggtgggtgcc gatatgcctt cgatgccgat ggcgcacgcg gtgaagccgg
    12061 tggccgccac ccccgcgggc gagccgggcc tgtatcgcgc ccgcatcaag ctggaaatga
    12121 cgggagaatg ggcgctgcac ctcaggctct ccgcgccgtt tcgcgaccgg atcgtcagcc
    12181 ggcagcgctt cgacgagtaa catgaggcca gacgcgccag ccggcttgat ctgcgccctg
    12241 ccttcacggc atcatgcgca tctttggtcc ggagacgggg gctccccatc ggatcgtcgg
    12301 gagaggagat cagaatgatg ccaagagcaa agcgcactga gcggcagaca ccgggccgcg
    12361 catactccaa gaccaccggc gcccgccgca agtttctcga ggctggggcc gcggttggcg
    12421 gagcgcttct gctgggagcg ccgcacatcc gcgacgcgcg ggccgccgcc ggccaggtct
    12481 ggaagatcca gtccacctgg gacgccggca ccaccggcta caagcttttc gaagagtggt
    12541 gcaacgggtt caaggagaag tccggcggcg agctggagct caagcccttc cccgccaagg
    12601 ccgtggccgc ggacaacaac gccctgttcg acgccgtgcg caccggcgtg ctgcatgcga
    12661 tgaacccgtt cacgctgtac tgggcgggca agattccagc gtcggtcttc ctgtcctcct
    12721 accccgcggg gccggatcaa cccagccagt gggacacgct gttctacggc ttgggcatgc
    12781 tcgaagccac gcgcgagatc ttcgccaagt tcgggctgta ttacgtgggc ccgatccagc
    12841 acgacgccaa catcatccac tccaagcgtc cggtcgccag cctggaccag ttgaaaggca
    12901 tgaaggtccg cctccccgga ggcatggtcg cggaggtgtt cgagaggttc ggcgtcagca
    12961 ccaccagcat gcccggctcg gacatctacc cggcgctgga gaagggcacg atcgacgccg
    13021 ccgactacgt cggccccgcc atcaactggg acctcgggtt ctcggaggtc accaagtaca
    13081 tcatctgcat ggggccgccg ggacagacct cgctctacca gcccgtggac ctgatggacc
    13141 tgacggtgaa tgcgggggtc tggaagcgcc tgtcgaagaa gatgcaggtg ttcgtcgaag
    13201 agcaggtgca gacctactcc gtgcaccact tcgtctcgat ccagaaggcg gacctggagg
    13261 cgatggccaa gttcctcaag tccggcacca cggtgtcgcg cctgtcggcc gaggacgtgg
    13321 tgcgcttccg ccgcgccgcc gttccggtct ggttcaactg ggcgaagaag aacgcggatg
    13381 cgacgcgcat cttcaagctc catctcgatt tcatgctcaa tccctacatg ggctatctca
    13441 cgcccgagga cctgaagggc ctcaagctgt aggccggcgc ttccgatcgg tcacgctctc
    13501 cctcccgcag ggggaggaga aggcggaagc ctgcgcacat cactcggggg ggagacatgt
    13561 cggagcacac caatttcgtc ctgccgcact ggctctattg gggctggctg ctgctgatgc
    13621 cgctggtgct gatgtggttc acgcgcggcg ccgccaccgc gggcgcgcca gacgagggaa
    13681 cggccgtcga ggtgctcgcc gaagaggatc cggcgctaca cgaccccggc aatcgtttca
    13741 cccgcgcgat cgactggctg agcgagcaat cgggcgtgtt cgtcgcgctg tggacggtga
    13801 acgcggtgtg cgtgtacttc tacgaggtca tttcgcgtta cctgttcgac gcgccgacga
    13861 tctgggcaca ccaggcctcc tacctgatgt tcggcatgca gtacctgctc gccggagcct
    13921 tcgcgctgtt gcacggcgcg cacgtgcgcg tcgatgtgct ctacgtcaag ctgccgccgc
    13981 gcggccagat cggcatggac atcttcacgt ccacgttttt cttcatcttc gcactggccc
    14041 tgctcggcag ctgctggcaa ttcttcgtca actcgctgga catgctcgag cgcaccgacg
    14101 agacctggca ggtccagttc tatccggtca agggcgtgat ggtgctgggg gcggtgctgc
    14161 tgaccctggc cggcgtgtcc aagctgatca aggatgtgag gctgttcgtg cgcaccggtg
    14221 ggagcgactc gagatgagca ccgccgctgc accaccggcc gcggtccgca agccgcgcgg
    14281 caagctgtgg acctggctga cctggctggt gaccgcaggc gtggcgctgg tggtcctggt
    14341 cgagatcgtc aacgtgctgt tctacgatcc cttcggggac gagcacttcc tgttcacgtt
    14401 gagcggcagc ctggagggcg tcggggtggg caccctcacc gcgctcatgt tcggctcgct
    14461 cgccgtgctg ctgatgatgg gactgccgat ggccttcgtg gtcggcggcc tgggcgtggt
    14521 gttcatctac ctggtcggcg gccaggcgat gatcaacatc atcccctcgc gcatcttccc
    14581 cctgatgacc aacccggacc tggcggctat cccgctgttc atcttcatgg ccaccatgct
    14641 cgagcgcgcc ggcctgatcg aggagatgtt cgacgtcgtc tacaagtgga tgggcgggct
    14701 gagcggcggc cttgcggcgg cgacgatcct cgcctccacc atcctggcgg cgatggtcgg
    14761 cgtgatcggc gcggcggtcg tgaccatggg catcatcgcg cttccggcca tgctcaggcg
    14821 caactacgat ccgcaactcg ccctgggttc gatcatggcg ggcggcacgc tgggcattct
    14881 catcccgcct tcgatcctgg cgatcctgta tgccgtggtg gcgcagcaat ccgtgggcaa
    14941 gctctatctc ggctgccttg ttcccgggct ccttctctcg gcgatgtaca tcgcttacgt
    15001 gctgattcgc acccgcctca ggcccgcatt gggcccggcg ctgcccaagg aggagcgcgt
    15061 gggcatgacc gagaagctgc gtctgctcgc ccgcctcgtc gcacccctgg tcctggtgtt
    15121 cttggtgctg gggctgctgt tcttcggcat cgccacgccg gtggaggccg ccggcgtggg
    15181 ctccttcggc gccatcgtgg tcgccgtcat gcacaagcgc ttcagcctga agtcgctgcg
    15241 cgaggcctcg atcaccactg cgcgcgcgtc ggcgatggtg ctgtggatca tcttcggggc
    15301 ctcgatcttc gtcggcttct tcatcctgca gggcggccag ggcttcatca ccgagagcat
    15361 cctcggcact ggcctgcccg cctacggcat cctggtcctg atgatggtgc tgctcgtctt
    15421 cctggggatg ttcctcgact gggtcggcat cctgctgctg gcggtgccga tcttcgtgcc
    15481 catcgtccag tcgctgacct tcgacggcct gctggggctg cccgcggtgc ctgccgcgga
    15541 ggtgacgctg tggttcggcg tgctgttcgt ggtgaacatg cagatgtcct tcctgagccc
    15601 accgttcggc tacgcgctgt tctacctgcg cggggtggct ccgcccgaga tcagcatggg
    15661 caccatcttc aagtcgtccc tggccttcct cgctctgcag gcgctcggct tgctgctttg
    15721 catcctcatc cccggactcg ccacctggct gccgcggcta gcgttcggat gacgctctgg
    15781 cgcgcatcgc gtcgcatccc gacggcgggc gcggtcgtcg caccggggca aaccccgcaa
    15841 ggagcactcg agatcttgcc gccgagacta tgataggcgc cgagcaccac cgtcagcgcc
    15901 tgcttctcac ggtcggaaac cacgtgctcg gcggcgccat cggtgccttc gaggcggacg
    15961 atggcgcggg ccgatgcggc aaccttgaat agcatcagga gatggtcggg ctgcaccagc
    16021 tcgtcgtacc agtagccacg ccagttggcg tcctcgcttt gtttcaggcg gggcgcggca
    16081 aactcgaagg acttgtcatc cgccttgatg tgaacgcggg tattgccagg gggcctgtcg
    16141 ccggggaact ggatgcggat gcgcagccat acttcgcagg ccttcttgcc gatatagagg
    16201 aagaacgcct cctcaccgac ccccgcgggc gaggactcat gccgatagag gatggcgccg
    16261 ctgtcgtcct tcacgacgcg cagactctcg gggatgtcgc cgaccacgcc acgcggacag
    16321 acttccgccg ggtgggggtc ctgcgcccaa gcagtggcag gcacgaacgc cgcgatccag
    16381 gcgcatagtg cgaacagcaa gcgtgtaggg attttgggat ctccgtcttt ggctgttcgg
    16441 gcgactgagc cggtggcgat gacgtttcgc gacccggcga tccgctgccc gggttcgaac
    16501 gcgctgaagg ctacgcgcac gatcgcaggg ggtgaagtat agacgaagcc gagcgaccgg
    16561 cgcgggcctc tgtctcgctc gcggtagaag ccgcgccgcc ggccatcgac cctgaggccg
    16621 gtcccggcct tgtgcctgag aagaccatgc cgtatagtcg aacgcagacg cgtgctccgc
    16681 tccgcacgga ccgagccgtt gccgtggtac ccgccgcgca gccactatga tctcgcgggt
    16741 gatcatgaag ctctcgccca acctccggct gttcgccctc ctgctgctcg ccttgacgtt
    16801 gtcagtcggc gtcgtggtgt tgtccgagag gacccaccgg cgcttgggcg aggccaatca
    16861 gcagatcgcc ggctccacgg aggcgcaggc gaccgccagc gaactgctcg cgctggtgat
    16921 cgatgccgaa acggcacagc gtggcctcct gctgacccag cgcccggagt atctcgaacc
    16981 ttatgtggtc gcgctgcccc agatccgtcc caagctggcc cggttgcgcg agttgactgc
    17041 cggcgcaacg gaagtccagg cgcacgtgga ccggctgggc gagctgctca gcgagcgctt
    17101 ctccgagctg gacgcctctc tggtgctata ccgcgatcga ggaccgaagg cggcgctcga
    17161 gctgatgcgc acggacttcg ggcgccgttc catggaccgg atccgcggcg aggtggacgc
    17221 gatccagcgc gcgctgcgca ctgacctcat cgagcgccac cagagctgga accgggacgt
    17281 cgccttctcg cgactggggc tggcaatcat cgccgggctg aacgtggcgc tgctgctgct
    17341 ggtgtacgtg caggcgcgca gagagatcct ctggcgcgag cgccagcgcc gcagtctcgt
    17401 ggagcagcgg cgcctgctgg aggagcaggt gcaggagcgc accgcgcagc tgtccgagct
    17461 gtccagcaac ctgcagaacg tccaggaggc cgagaaggct aagctcgcgc gcgacatcca
    17521 tgacgagctc ggcagcatcc tggtgtcggc gcgcatggat gtgtcctggg cgcggcggcg
    17581 ggtcgagccg ctcgatcccg cggccgcgga aaagctggcg cgcgccagca gcactctgga
    17641 cgaaggcgtt gagatcaagc gccgcatcat cgaagacctg cggcccacat tgttggacaa
    17701 cctgggcctg gccgcggcgt tgaactggca cgtgaaccag gcgtgcgaac gcggcgggtt
    17761 ggcctgcaat ctgtcgttgc cggaagaaga tctcgaggtc cccagtcccg tttcgatcgc
    17821 gctgtttcgc gttgtgcagg aggcgctcac caacgtcttg cgctacgcga aagcgcgcaa
    17881 cgtctggctc tccgtcgagc acacggagac cggtatctcc ctggctctgc gcgacgacgg
    17941 cgtgggcata gccccgcggg cgttgggcga tcgtctttca catgggatcg tggggatgcg
    18001 ccagcgcatc gcctcgctcg gaggaacctt ccgcatcggg cgagtcgccc aggggggcac
    18061 cgccatcgag gtctttgttc cactgcacgg ccgcgacgcg cccgccgacg acgaaggggc
    18121 gcccgttggg ccgcagcagg cggaaagcat ccccgagtcg taacccgcat cacgcatcgg
    18181 gacgcccccg gagccacggg cctctcctgg cgccttggcg gcggaaccgg ggggttg
//
LOCUS       DASPGE010000314         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1721522, whole genome shotgun sequence.
ACCESSION   DASPGE010000314 DASPGE010000000
VERSION     DASPGE010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721522"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VD791_10120"
     CDS             complement(<1..213)
                     /locus_tag="VD791_10120"
                     /inference="COORDINATES: protein motif:HMM:NF020013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08373.1"
                     /translation="MAIYRTETACRVCGGEGLVEILAFGASPLADRLLRRDQLREPDV
                     AVPLTLMFCPACSLVQIAETVAPEVLF"
     gene            complement(230..1276)
                     /locus_tag="VD791_10125"
     CDS             complement(230..1276)
                     /locus_tag="VD791_10125"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV08374.1"
                     /translation="MRVLLTGNEGYIGTLLVPLLWARNHEVVGLDSGLFRECTLKAIR
                     PLEQTMRKDIRDVEPQDLAGIDAVIHLAGLSNDPLGSLAPRFTYEINHAATVRLAEIA
                     KRAGIRRFLYASTCSVYGAAGDEVLDEDSPLNPVTPYAESKARAERDLRPLADATFCP
                     VYLRAATAYGVSPLLRFDLAVNNLVAWAATTGQVYLKSLGTSWRPLVHIEDIALAYIT
                     LLHAPAAKVHNLAFNIGRTEENYRIRDVAEIVRRTVPHTRIEFAPDASPDVRNYRVSC
                     DRIAREVPEYRPHWSVAQGVAEVYRGILEWGLHRDDFEGPRYNRIAYLKQLRAQGRVA
                     DDLRWADPSAVAAE"
     gene            complement(1286..2044)
                     /locus_tag="VD791_10130"
     CDS             complement(1286..2044)
                     /locus_tag="VD791_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008453229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HXV08375.1"
                     /translation="MKVAILAGGQGTRLAEETRVKSKAMVRIGEQPILWHLLKYYAHY
                     GYTQFVIALGYHADSIREYFIEGLDAVPVGDGVRTVLQPAAEPRWTVELIDTGLETMS
                     GGRLKRLAPYLGGAAFMLTWCDGLADVDLHRLRACHEAHGRLATLTAVHPPARFGRLT
                     LAGDRISAFCEKTLDPDAWINGAFFVLEPGVFDYIEGDDTQFERQPLSRLAAAGELMA
                     HRHEGFWQCMDTLKEAQDLNALWREGTAPWRIWP"
BASE COUNT          380 a          857 c          776 g          367 t
ORIGIN      
        1 gaacaggacc tcgggagcca cggtctcggc aatctgcacc aggctgcacg ccgggcagaa
       61 catcagggtg agcggcaccg cgacatccgg ctcgcgcagc tggtcccggc gcagcagccg
      121 gtcggccagc ggactggccc cgaacgccag gatctccacc agaccctcgc cgccgcagac
      181 gcggcaggcg gtctctgttc ggtagatcgc catgggcgcc cgtccggcct cactccgctg
      241 cgaccgcgct cggatcggcc cagcgcaggt cgtcggcgac ccggccctgc gcgcgcagct
      301 gcttcagata ggcgatccgg ttgtagcgcg ggccctcgaa gtcgtcgcgg tgcagccccc
      361 attcgaggat gccccggtag acctccgcga cgccctgcgc gaccgaccag tgcggccggt
      421 actccggcac ttcgcgcgcg atccggtcgc agctcacccg gtagttgcgc acatccggcg
      481 aggcgtccgg cgcgaactcg atgcgggtgt gcggcaccgt ccggcgcacg atctcggcga
      541 cgtcgcgaat gcggtagttc tcctcggtgc gcccgatgtt gaacgccagg ttgtgcacct
      601 tggccgcggg tgcgtgcagc agcgtgatgt aggcgagcgc gatgtcctcg atgtgcacca
      661 gcggccgcca cgaggtgccg aggctcttca gatacacctg cccggtggtc gccgcccagg
      721 cgaccagatt gttgaccgcg aggtcgaagc gcagcagcgg cgacacgcca taggcggtgg
      781 cggcgcgcag atacaccggg caaaacgtcg cgtccgccag cggccgcagg tcccgctccg
      841 cgcgcgcctt ggattcggcg tacggcgtga ccggattgag cgggctgtcc tcatccagca
      901 cctcgtcgcc ggcggcgccg tacacgctgc aggtcgaggc atacaaaaag cgccggatgc
      961 cggcgcgctt ggcgatctcg gccagacgca cggtggccgc gtgattgatc tcgtaggtga
     1021 agcgcggcgc gaggctgccg agcgggtcgt tggacagacc cgcgagatgg atcaccgcat
     1081 cgatgcccgc gaggtcttgc ggctcgacgt cgcggatgtc cttgcgcatg gtctgctcga
     1141 gcgggcggat cgccttgagc gtgcactcgc ggaacagccc gctgtccaac cccaccacct
     1201 cgtggttgcg cgcccacaag agcggcacca acagcgtgcc gatgtagcct tcgttgccgg
     1261 tcaacaagac gcgcatcgtt cctccctatg gccagatgcg ccagggcgcc gtgccctcgc
     1321 gccacagcgc gttcaggtcc tgcgcctcct tgagcgtgtc catgcactgc cagaagccct
     1381 cgtggcgatg cgccatcagc tcgcctgcgg cggccagccg gctgagcggc tggcgctcga
     1441 actgggtgtc gtcgccctcg atgtagtcga acaccccggg ctcgagcacg aagaacgccc
     1501 cgttgatcca cgcatccgga tcgagcgtct tctcgcagaa ggcgctgatc cgatcgcccg
     1561 ccagcgtcag gcggccgaag cgcgccggcg ggtggaccgc ggtcaaggtc gccagccggc
     1621 cgtgcgcctc gtggcaggcg cgcaaccggt gcaggtcgac gtcggccagg ccgtcgcacc
     1681 aggtcagcat gaaggcggcg ccgcccagat agggggccag gcgcttgagg cgcccgccgg
     1741 acatggtctc caggccggtg tcgatcagct cgaccgtcca cctgggctcc gccgccggct
     1801 gcagcaccgt ccgcacgccg tcgccgaccg gcacggcatc cagcccctcg atgaagtatt
     1861 ccctgatcga gtccgcgtgg tagccgagcg cgatcacgaa ctgcgtgtag ccatagtgcg
     1921 cgtaatactt cagcagatgc cacaggatcg gctgctcgcc gatgcgcacc atcgccttgg
     1981 acttgacccg cgtctcctcc gccagccgcg tcccctgccc acccgcaaga atcgccacct
     2041 tcatgccacg tcaccgcagc tcaaactgcg tgcccgccac agacgggttg gcacttcaag
     2101 acggaccatc ggacgatgct tgtgacaaca tgcctggcta ccctcccgac tccacgtgtg
     2161 cacactcaag ttgccgtcga gcaggcgaca cgtctgtcgg atagccgaca cttccgtcgg
     2221 acatgacggg cacccgcgtg ccgggcatct ccaggtccat ttcaacgagc tccgcatcga
     2281 cgccgaggcc agggcctgcc ctcaggcgaa tcgatatatg tccagtgcga gcggaaacgg
     2341 ctcgagctcg cgctgttcaa tcccattccc tgatcgcccc
//
LOCUS       DASPGE010000315         6170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1486181, whole genome shotgun sequence.
ACCESSION   DASPGE010000315 DASPGE010000000
VERSION     DASPGE010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486181"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VD791_10135"
     CDS             <1..253
                     /locus_tag="VD791_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXV08376.1"
                     /translation="YQRGLGQQIVDTAIATLRDRGVPHQGRVAIGEIAETIVATAQEL
                     GAEQIVIGSRGMSALPNLLLGSNATKVIHLARVPVTIVK"
     gene            complement(280..1623)
                     /locus_tag="VD791_10140"
     CDS             complement(280..1623)
                     /locus_tag="VD791_10140"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXV08377.1"
                     /translation="MSETLIAPAVAEIMGPGVAASILFGGFFALLFLRVPVAFALGLA
                     CLPIFLWDERLSPQNLLQETFNAYNSFILLAVPFFLLTANLMNVGGITDRLVRLSRAL
                     VGHFPGGLAQINVVLSIFFAGISGSSTADAASQSKIFIEAQRKEGYDDGFSVAITAVS
                     AVLAVIIPPSILMIVWGGLLTVSIGGLFLAGIVPGLLIGFVQMGTVHAYARARGYPTY
                     PRAALLEVVKALAVSVPALMTPVIIIGGKIFGWVTATESACIAVLYAGVLSALVYREM
                     NLAGLQRALLDTGRLAAVALFCVGTASAFGWLLAYYQIPKALLAGVQAWHMGATATGF
                     FISAVFLVVGCFLDAIPAIIIVGTILAPLAQSAGLDPIHFAMIGIVSLAFGLVTPPYG
                     LCLMIACAIAGMRVLDALKDTLIMLIPMFAVLALVIVWPDLVLFLPRLVSPEFLQ"
     gene            complement(1620..2129)
                     /locus_tag="VD791_10145"
     CDS             complement(1620..2129)
                     /locus_tag="VD791_10145"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXV08378.1"
                     /translation="MSSEPSPRSAWRRLTAGYARLLTWLLAASVAVLIVPVSLQIFSR
                     YTALIPAYIWTEEMARFLFVWMIMLGAMLGVRESSHFVVDVWPRMGSRAQAALRLVAN
                     LFVLVAALAFLWWGIEFTRFALGRLSELAELPLWLIHVAWPLAGASWIAFLGEQLYDD
                     LRTAFGGVR"
     gene            complement(2228..3220)
                     /locus_tag="VD791_10150"
     CDS             complement(2228..3220)
                     /locus_tag="VD791_10150"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXV08379.1"
                     /translation="MTRRAFLRRIAAGAALAAAARPAGAQVLTLHGASQFNDDHAFTK
                     ALVRFEELVKKYYGKPVGFALHKNSELGLEKQYFEYMAQGKAVDYAIVSPAHMSTFSR
                     TAPFIDAPFLFRDLAHWRKVLDQDLLKPVADEIAQKADVLLLGYAGGGIRNIFASKPL
                     PDLAALRGLKVRVQGAPIWSRTVAAAGMAPTVIAYNEVYNAIQNGVIQAGENEAAGVE
                     QMRFYEVAPHLALTQHAITIRPLCFSGKTFKRLPPDLQAAIRKAGREAGEYGREVESS
                     EDARKLDALEKAGKLKRVAFAGRERMKQLVDPVMAAYARDIGASSVLDRINAIK"
     gene            3349..4002
                     /locus_tag="VD791_10155"
     CDS             3349..4002
                     /locus_tag="VD791_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08380.1"
                     /translation="MYLCAPGRHVAAMLALLPLLAACLMANQRYSTPHTEDFGLAAGQ
                     LESGGLGFLTPSTVTGQEEDRQPLALVVADVLAEQRKEIRTVGLPETLSAVNRAGLTE
                     AYKRMYQDYRDSGVFEPKVLRQVGDAVGVRYMVQLKMAGFRQDSRERFGLLGLSILQT
                     QAANIRLFLQIWDTDDGSIVWEGNNELNRAFDTFSERSVSFDTVVRESASELVAKLP"
     gene            complement(4119..4937)
                     /locus_tag="VD791_10160"
     CDS             complement(4119..4937)
                     /locus_tag="VD791_10160"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HXV08381.1"
                     /translation="MIVHLLDGTYELFRHFYGLRRFTKGEDPPFGAVIGVLQTVLQTI
                     ETGETHVGVATDHVIESFRNRLWPGYKTGDGIEPALRAQFQPLEDALAAMGVAVWPMT
                     ELEADDALASAAHAAAQDARVQKVCIWTPDKDLAQCVRGDRVVQVDRRQKKVLNAEGV
                     RAKFGVEPALIPDYLALVGDAADGYPGIPGIGAVTAARLLNQHGSIENFPRALLEDDR
                     DLALLFKKLATLRIDARLFEDVDELRWNGPGAGFGACAERIGAARLLKRALAAK"
     gene            complement(5044..5796)
                     /locus_tag="VD791_10165"
     CDS             complement(5044..5796)
                     /locus_tag="VD791_10165"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003279366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HXV08382.1"
                     /translation="MIQRVALVTGSSRGIGRAIALRLARDGAAVAVNYRERRDQAEEV
                     VEAIGRSGGRAVAVAGDLAKAEDCTRLVETARQALGPIDILVNNAGVFRKGDLADFDF
                     AQFHGMREINVDGLVRVTHAVAGDMRARRWGRIVNIASIAGLGTAAAGTTFYAATKAA
                     VITLTRRFALDLGPDGITVNAIAPGFIATEMAAQSGADFDTVAAKAMMRRIGTPDDIA
                     AAASFLASDQAGFITAQVLTVDGGRTDFIGHP"
     gene            complement(5945..>6170)
                     /locus_tag="VD791_10170"
     CDS             complement(5945..>6170)
                     /locus_tag="VD791_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HXV08383.1"
                     /translation="GRARVCVANSVFVLAHAGAPRRACTMFRGWFAGALEFVGAQLGN
                     RIDLASEEIRCAAQGPLGRCVFEIAPKADR"
BASE COUNT         1024 a         2087 c         2144 g          915 t
ORIGIN      
        1 gtaccagcgt gggctgggtc agcagatcgt cgacacggcc atcgccacct tgcgggaccg
       61 cggcgtgccg caccagggcc gcgtggccat cggcgagatc gcggagacca tcgtggccac
      121 ggcgcaggag ctcggcgccg agcagatcgt gattggctcg cgcgggatgt ccgccttgcc
      181 gaacctgctg ctcggttcga acgccacgaa ggtcatccac ctggcgcggg tgccggtcac
      241 gatcgtgaag tgacccgcgc ggctcgccga cccgcaccct cactgcagaa actcggggga
      301 gacgagccgg ggcaggaaca ggaccagatc gggccacacg atcaccagcg ccagcaccgc
      361 gaacatcggg atcagcatga tcagcgtgtc cttgagcgcg tccagcacgc gcatgcccgc
      421 gatcgcgcag gcgatcatca ggcacaggcc gtagggcggt gtgaccaggc cgaaggcgag
      481 cgagacgatg ccgatcatgg cgaagtggat cggatcgaga ccggcggact gcgcgagcgg
      541 cgcgaggatg gtgccgacga tgatgatggc ggggatcgcg tccaggaagc agcccaccac
      601 caggaacacc gcggagatga agaagccggt agccgtggcg cccatgtgcc aggcctgcac
      661 gccggcgagc agcgccttgg ggatctggta gtaggcgagc agccagccga aggcgctcgc
      721 cgtgccgacg cagaacagcg ccaccgcggc cagccggccg gtgtcgagca gcgcccgctg
      781 cagtcccgcg agattcatct cgcggtacac cagcgccgac agcacgccgg catacagcac
      841 cgcgatgcag gccgactcgg tggcggtgac ccagccgaag atcttgccgc cgatgatgat
      901 caccggcgtc atcaacgccg gcaccgagac ggccagcgcc ttcaccacct ccagcagcgc
      961 ggcgcgcgga taagtcggat agccgcgcgc cctggcatag gcgtgcacgg tgcccatctg
     1021 cacgaagccg atcagcaatc cgggaacgat gccggccagg aacaagccgc cgatcgacac
     1081 ggtgagcagc ccgccccaca cgatcatcag gatcgagggc gggatgatga cggcgagcac
     1141 cgccgagacc gccgtgatcg ccaccgagaa gccgtcgtcg tagccttcct tgcgctgcgc
     1201 ctcgatgaag atcttcgact ggctggcggc gtccgcggtg gaggagccgg agatgccggc
     1261 gaagaagatc gacagcacca cgttgatctg cgccagcccc ccggggaagt gcccgaccag
     1321 tgcgcgcgac agccgcacca ggcggtcggt gatgccgccg acgttcatca ggttggcggt
     1381 gagcaggaag aacggcaccg ccagcaggat gaaggagttg taggcgttga acgtctcctg
     1441 cagcaggttc tgcggcgaca ggcgctcgtc ccacaggaag atgggcaggc aggcgagccc
     1501 cagggcgaag gccaccggca cgcgcaggaa cagcagcgcg aagaagccgc cgaacaggat
     1561 ggacgccgcc acgcccggac ccatgatctc tgcgactgcg ggcgcgatca gcgtctcgct
     1621 caacgcacgc ctccgaacgc ggtgcgcagg tcgtcgtaga gctgctcgcc gaggaacgcg
     1681 atccagctcg cgccggccag cggccacgcg acgtggatca gccacagcgg cagctcggcc
     1741 agctcggaca ggcggccgag cgcgaagcgg gtgaactcga tgccccacca caggaacgcc
     1801 agggcggcga ccagcacgaa caggttcgcc accaggcgca gcgccgcctg cgcgcgcgag
     1861 cccatgcgcg gccacacgtc caccacgaag tggctgctct cgcgcactcc cagcatggcg
     1921 ccgagcatga tcatccacac gaacaggaag cgcgccatct cctcggtcca gatgtaggcc
     1981 gggatcagcg cggtgtagcg cgagaagatc tgcaggctca ccgggacgat gagcacggcg
     2041 acgctcgccg ccagcagcca ggtcagcagg cgcgcgtagc ccgcggtcag gcgccgccag
     2101 gcgctgcgcg gggagggttc gctggacatt ggctgggatg gttcacgagc cgcgtagccc
     2161 ggatgcagcc gcccgagatc ttgcgggcca agcgggcggc ggaatccggg aggcctttct
     2221 ctgccgctta cttgatggcg ttgatccggt cgaggaccga cgaggcgccg atgtccctgg
     2281 cgtatgctgc catcaccggg tcgaccagct gcttcatgcg ttcgcgtccg gcgaaggcga
     2341 cgcgcttgag cttgccggcc ttctccagcg cgtcgagctt gcgcgcatcc tcgctcgact
     2401 cgacctcgcg gccgtactcg ccggcctcgc gaccggcctt gcggatcgcc gcctgcaggt
     2461 ccggcggcag gcgcttgaag gtcttgcccg agaagcacag cgggcggatg gtgatggcgt
     2521 gctgcgtgag cgccaggtgc ggcgcgacct cgtagaagcg catctgctcc acgcccgcgg
     2581 cctcgttctc gccggcctgg atcacgccgt tctggatggc gttgtagacc tcgttgtagg
     2641 cgatcacggt gggcgccatg ccggccgcgg cgacggtgcg gctccaaatc ggcgcgccct
     2701 gcacgcgcac tttcaggccg cgcagcgccg ccaggtccgg cagcggcttg ctggcgaaga
     2761 tgttgcggat gccgccgccg gcgtagccca gcagcagcac gtccgccttc tgcgcgatct
     2821 cgtcggccac cggcttgagc agatcctggt cgagcacctt gcgccagtgg gccaggtcgc
     2881 ggaacaggaa gggtgcgtcg atgaacggcg cggtgcgcga gaaggtggac atgtgcgccg
     2941 gcgagacgat ggcgtaatcc accgccttgc cctgcgccat gtactcgaag tactgcttct
     3001 ccagccccag ctcgctgttc ttgtgcagcg cgaagccgac cggcttgccg tagtacttct
     3061 tcaccagctc ctcgaacctc accagcgcct tggtgaaggc gtggtcgtcg ttgaactggg
     3121 aggcgccgtg cagggtcagg acttgcgcgc cggccggccg cgcggcggcg gccagggccg
     3181 cgcccgcggc gatgcggcgc aggaatgcgc gtcgggtcat gcggcctcct ccgtgttgtc
     3241 cgcccgggtc atggcgcccg gtgcctagcg tcgattgtcg gcgattcgcg tcaccggctc
     3301 aagtgacggg gcccgcgcgc gtggcccggg ctcgggtaga ctgcgcccat gtacctctgc
     3361 gcgcccggac gccatgtcgc cgccatgctg gccctgctgc cgctgcttgc ggcttgcctc
     3421 atggccaacc agcgctactc cacgccgcat accgaggact tcgggctggc cgcaggccag
     3481 ctcgaaagcg gcgggcttgg ctttctgacg ccgtcgactg tcaccggtca ggaggaggac
     3541 cggcagcccc tggcgctggt ggtcgccgac gtgctggccg agcagcgcaa ggagattcgc
     3601 accgtgggat tgccggaaac gctgagcgcg gtgaatcgtg cgggccttac cgaagcgtac
     3661 aagcgcatgt accaggatta ccgggacagc ggcgtgttcg agcccaaggt gttgcgccag
     3721 gtcggagacg ccgtcggcgt gcgttacatg gtgcagctca agatggccgg cttccgccag
     3781 gactccaggg aacgcttcgg gttgctcgga ctgtcgatcc tgcagaccca ggctgcgaac
     3841 atccgactgt tcctgcagat ctgggacacc gatgacggca gcatcgtctg ggagggcaac
     3901 aacgagctca atcgcgcctt cgacacgttc agcgagcgca gcgtgtcgtt cgacaccgtg
     3961 gtgcgcgagt cggcatcgga gctggtcgcg aagctgcctt gagcgtggca gtcacgaggg
     4021 gctcgcaagc cgcattttat tgaagttttc gtaagtgcgt gacgaaagcc acaccacccc
     4081 cggccttcgg ccgacccctc ctcatgagag gaggggagtc acttcgcagc gagggcgcgt
     4141 ttcagcagac gggctgcgcc gattcgctcc gcgcaggcgc cgaaaccggc gccgggaccg
     4201 ttccatcgca gctcgtccac gtcttcgaac aggcgcgcgt cgatccgcag cgtcgcgagc
     4261 ttcttgaaca gcagcgccag gtcccggtcg tcttccagta gcgcgcgcgg aaagttctcg
     4321 atgctgccgt gctggttgag gagccgcgcg gccgtgacgg ctccgatccc gggaatgccc
     4381 ggatatccgt ccgccgcatc gccgaccagc gccaggtagt ccggaatcaa ggcgggctcc
     4441 acgccgaact tggcgcgcac gccttccgcg ttcagcacct tcttctgcct gcggtccacc
     4501 tgcaccaccc ggtcgccgcg cacgcactgc gcaaggtcct tgtcgggcgt ccagatgcag
     4561 accttttgca cgcgcgcgtc ttgcgcggcg gcgtgcgcgg cggaggcgag cgcatcgtcc
     4621 gcctccagct cggtcatggg ccatacggcc acgcccatcg cggcgagcgc gtcttcgagc
     4681 ggttggaact gcgcgcgcag cgcaggctcg atgccgtcgc ccgtcttgta gccgggccag
     4741 aggcggttgc ggaacgattc gatgacgtga tcggtcgcca cgccgacgtg cgtctctccc
     4801 gtctcgatcg tctgcagcac ggtctgcagc acgccgatca cggcgccgaa cggcggatcc
     4861 tcccccttgg tgaagcggcg cagaccgtag aagtgacgga acagctcgta cgtgccgtcc
     4921 aagaggtgaa cgatcatcgt gccattatcg ccccggtgcg tcagccgagc ttcgccgcca
     4981 tgctccgctc tcggctcgcg cgacagcggg ggtaaggggg cggaagcgcc cggccgcgcg
     5041 actctatgga tgcccgatga agtcggtgcg gccgccgtcg acggtgagga cctgggcggt
     5101 gatgaatccg gcctgatcgg aggcgaggaa cgacgccgcg gcggcgatgt cgtccggtgt
     5161 gccgatgcgt cgcatcatcg ccttggcggc cacagtgtcg aaatccgcgc cgctctgggc
     5221 ggccatctcg gtggcgatga aaccgggggc gatcgcgttc acggtgatgc catcggggcc
     5281 caggtcgagg gcgaagcggc gcgtgagcgt gatgacggcg gccttggtgg ccgcgtagaa
     5341 cgtggtgccc gccgcggcgg tgcccagccc ggcgatggaa gcgatgttga cgatgcggcc
     5401 ccagcggcgg gctcgcatgt cgccggcgac cgcgtgcgtg acgcgcacca gcccgtcgac
     5461 gttgatctcg cgcatgccgt ggaactgcgc gaaatcgaaa tcggcgagat cgcccttgcg
     5521 gaagacgccg gcgttgttca ccaggatgtc gatggggccg agcgcttggc gtgcggtttc
     5581 gaccagccgc gtgcaatcct cggccttcgc aaggtcgccc gcgacggcca ccgcgcggcc
     5641 gccggagcgc ccgatggcct cgaccacttc ctcggcctgg tcgcggcgct cgcggtagtt
     5701 gacggcaacc gcagccccgt cccgggcgag gcgaagagcg atcgcgcgcc cgatgccgcg
     5761 cgacgatccg gtaaccaaag cgactctctg gatcatcggc gcattgtacc gggtcggacc
     5821 acgctaagcc tctgaagcct gcgcactttg ttcccaatcg aggcgatttt tcgagcttag
     5881 agggtctatt tcggcccgaa aaacggcccg ctaagcctgc aacgatgcgg tccgttgcct
     5941 gggatcaacg gtccgccttc ggagcgatct cgaacacgca gcggcccagc gggccctgcg
     6001 ccgcacaccg gatctcctcg gacgcgaggt cgatgcggtt tcccagctga gcgccgacga
     6061 attcgagggc acccgcgaac cagccgcgga acatggtgca ggccctgcgc ggcgcacccg
     6121 catgcgccag cacgaagacc gaattggcca cgcacacccg ggcgcgcccg
//
LOCUS       DASPGE010000316         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1252453, whole genome shotgun sequence.
ACCESSION   DASPGE010000316 DASPGE010000000
VERSION     DASPGE010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252453"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..612)
                     /locus_tag="VD791_10175"
     CDS             complement(166..612)
                     /locus_tag="VD791_10175"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HXV08384.1"
                     /translation="MAQATRLALALLAAAWLQLAPLAADAASPPKDRRWSELSAEQQK
                     ILAPLQQEWDALEPEQRRKWIGVAKRYPKFTPTGQKRTQTRMKKWAKLTPEERKQARE
                     NYRRISKVPPDKRGDLKQQWSEYQALPPQERQKLQSSSKPKSTTTR"
     gene            complement(585..965)
                     /locus_tag="VD791_10180"
     CDS             complement(585..965)
                     /locus_tag="VD791_10180"
                     /inference="COORDINATES: protein motif:HMM:NF023699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HXV08385.1"
                     /translation="MDHETHFAHRLRPYLNEGARVDARVAARLRAAREQALAAYRHER
                     QDWVALPAGAGGRLGGLRTLWLRIVLPAVVLCVAFAATYAWQQQQRAAELEELDALLL
                     TSDLPIDAYLDQGFAAWLKRRASH"
     gene            complement(968..1537)
                     /locus_tag="VD791_10185"
     CDS             complement(968..1537)
                     /locus_tag="VD791_10185"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV08386.1"
                     /translation="MASRSELSTFLEGAERRAFKQAVYAVRDEQAALDVVQDAMLRLV
                     ETYGGRPVAELPMLFQRILQNAIRDWFRRQKVRSTWIALFSTLLEARGIADDADLLET
                     LPPGREPNDDERPGAALERTQVIEIIEQEIAQLPSRQRQAFLMRYWEELDVADTARAM
                     GCSEGSVKTHCSRAAHALAVALRARGITL"
     gene            1676..>2337
                     /locus_tag="VD791_10190"
     CDS             1676..>2337
                     /locus_tag="VD791_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HXV08387.1"
                     /translation="MDRIKGVELSGAEIVVRCLQEEGVEYVFGYPGGAVLYIYDELFK
                     QDKVRHVLVRHEQGAVHAADGYSRSSQKVGVCLVTSGPGVTNAVTGIATAYMDSIPMV
                     VLTGQVPTHAIGQDAFQECDTVGITRPCVKHNFLVKDVKDLAATIKKAFYLATTGRPG
                     PVLVDIPKDVTAHQCEFHYPDSVAMRSYNPVRKGHAGQIKKAVQLILEAERPMVYTGG
                     GVV"
BASE COUNT          357 a          806 c          801 g          373 t
ORIGIN      
        1 cagccaccac cagcgcttgg tgcgcgagca gcacccagcc ttccaggggc acgccgccgg
       61 aggcgaacag gaacgcgaag ccggcgaaga aggccaccgc gaacaccagc accgcctcgt
      121 acagcaggca ggcgaggcgg cgcgcgagcc cgggagtgcc ggggctcacc gcgtggtcgt
      181 ggacttcggc ttgctgctcg actgcagctt ctggcgctcc tgcggcggca gcgcctggta
      241 ctcggaccac tgctgcttca ggtcgccgcg cttgtcgggc ggcaccttgc tgatgcggcg
      301 gtagttctcg cgcgcctgct tgcgctcctc cggcgtgagc ttggcccatt tcttcatgcg
      361 cgtctgcgtg cgcttctggc cggtcggcgt gaacttcggg tagcgcttgg ccaccccgat
      421 ccatttgcgc cgctgctccg gctcgagggc gtcccactcc tgctgcaggg gcgcgaggat
      481 cttctgctgc tcggcgctca gctccgacca gcggcggtcc ttcggcggcg acgcggcatc
      541 ggcggcgagc ggcgccagct gcagccaggc ggctgccagc agcgctagtg cgaggcgcgt
      601 cgcttgagcc acgccgcgaa tccctggtcc aggtaggcgt cgatcggcag gtcgctcgtc
      661 aggagcaggg cgtccagctc ctcgagctcg gcggcgcgct gctgctgctg ccaggcgtag
      721 gtcgcagcga atgccacgca cagcacgacg gccggcagca cgatgcgcaa ccagagcgtg
      781 cgcaggccgc cgagccggcc gcccgcgccc gccggcagcg ccacccagtc ctgacgctcg
      841 tggcggtagg ccgcgagcgc ctgctcgcgc gcggcacgca ggcgcgcggc cacgcgcgcg
      901 tccacccgcg caccctcgtt caggtacggg cgcaggcggt gggcgaagtg cgtttcgtgg
      961 tccatgacta aagcgtaatg ccgcgcgcgc gcaatgcaac tgccagcgca tgcgccgccc
     1021 gcgagcaatg cgtcttcacg ctgccctcgg agcagcccat ggcgcgggcg gtgtcggcga
     1081 catccagttc ctcccagtaa cgcatcagga acgcctgccg ttgacgcgat ggcaactgcg
     1141 caatctcttg ttcaatgatt tcaatgactt gagttctctc cagggccgcg cccggccgtt
     1201 catcgtcatt gggctcgcgg ccgggcggca gcgtttccag cagatccgcg tcgtcggcga
     1261 tccctcgagc ttcgagcagc gtggaaaaga gcgcgatcca cgtcgagcgc accttctggc
     1321 gccggaacca gtcccggatg gcgttctgca ggatgcgctg gaacagcatc ggcagctccg
     1381 ccaccggccg gccgccgtac gtttccacga ggcgcagcat ggcgtcctgc acgacgtcga
     1441 gcgccgcctg ctcgtcgcgc accgcataga cggcctgctt gaaggctcgc cgttcggcgc
     1501 cttcgaggaa ggtcgaaagc tcgctgcgcg aggccagtgg tgctcccttc ctccgacgcc
     1561 ggggtgcgtg tggcgggcga tgttaccaaa acgggctcgg cggccatcgg cggattgctt
     1621 gaccgcaatg caacaagtct ttaatgttca acgttttccc cgggtctttg cgcgcatgga
     1681 tcgcatcaag ggagtcgagc tgagcggcgc cgaaatcgtg gtgcgctgcc tgcaggagga
     1741 gggcgtcgag tacgtcttcg gctacccggg cggcgccgtg ctctacatct acgacgagct
     1801 gttcaagcag gacaaggtgc gtcacgtgct cgtgcggcac gagcagggcg cggtgcatgc
     1861 ggccgacggc tactcgcgct cgtcgcagaa ggtcggcgtc tgcctggtga cctcgggacc
     1921 gggcgtcacg aacgccgtca ccggcatcgc cacggcctac atggactcga tcccgatggt
     1981 ggtgctcacc ggccaggtgc cgacgcacgc catcggccag gacgccttcc aggaatgcga
     2041 caccgtcggc atcacgcgcc cgtgcgtcaa gcacaacttc ctggtgaagg acgtcaagga
     2101 cctggcggcg accatcaaga aggccttcta cctcgctacg accgggcggc ccgggccggt
     2161 gctggtggac atcccgaagg acgtgacggc gcaccaatgc gagttccact acccggactc
     2221 ggtcgccatg cgctcgtaca acccggtgcg caagggccat gccgggcaga tcaagaaggc
     2281 ggtgcagctc atcctcgagg ccgagcgccc gatggtgtac accggcggcg gcgtggt
//
LOCUS       DASPGE010000317        12032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1486411, whole genome shotgun sequence.
ACCESSION   DASPGE010000317 DASPGE010000000
VERSION     DASPGE010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12032
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486411"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="VD791_10195"
     CDS             <1..1042
                     /locus_tag="VD791_10195"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HXV08388.1"
                     /translation="PSACGKTNFAMLIPPPAFGGWKVTTIGDDIAWIKPGADGRLYAI
                     NPEFGFFGVAPGTSPKSNPNALATLARNCVFTNVALTEDGDVWWEDLTEQPPARLVDW
                     QGQPWTPDCGRTAAHPNARFTVPAAQCPVIDPDWEKPEGVPISAFIFGGRRSRSIPLV
                     YETFHWVDGVYAAATMGSETTAAAAGKVGEVRRDPFAMLPFCGYHIGDYFDHWLEIGR
                     RVPNPPRVFCVNWFRRGDDGAFLWPGYGENMRVLKWIVDRCRGRAGATQSVLGWMPRE
                     HDLEWAGLDAGVRRRYPELMSIERDAWIHELLMHEELFVRLYDKLPKEFVLKRELLLA
                     SLWRSPERWTVP"
     gene            complement(1089..2078)
                     /gene="rfaD"
                     /locus_tag="VD791_10200"
     CDS             complement(1089..2078)
                     /gene="rfaD"
                     /locus_tag="VD791_10200"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HXV08389.1"
                     /translation="MIVVTGAAGFVGSNLVKALNRRGDTDIVAVDNLARGDKFRNLVD
                     CELADYLDKDAFRQALAAGDFDGEVSAVLHQGACSDTLEADGRYLLDNNYRCSLELLE
                     FCRSEDIPFLYASSAAVYGAGPAFREDAQFEKPLNVYGYSKLLLDQVVRRRLGESSAQ
                     AVGLRYFDVYGPREAHKARMASVAWHFFNQYRTQGRVQLFEGSGGYAAGEQRRDFVFV
                     EDVVAVNLFFLDHPGRSGIFNVGTGRAQTFNELAVATINAVRASQGKDALPLEEMLAQ
                     NVIDYIPFPAGLPDRYQSFTQADLTQLRQAGYSAPFLSVEQGVRRYVDWLLKQ"
     gene            complement(2095..3024)
                     /gene="rfaE1"
                     /locus_tag="VD791_10205"
     CDS             complement(2095..3024)
                     /gene="rfaE1"
                     /locus_tag="VD791_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HXV08390.1"
                     /translation="MSIDRALLSKARLLVVGDVMLDQYWFGEVSRISPEAPVPVVRIG
                     RMEERPGGAANVARNATALGAQVQLLAVIGEDEPGASLERLLAAEGVHVHLTRDPTLS
                     TTVKLRVVGRQQQLVRIDFENLPAANALEAKLADFESRLPLAQVVILSDYAKGALTHV
                     ERMIQATRAAGRPVLIDPKGDDYDKYRYATLLTPNRNEFRTVVGSWKSDADFEARGQR
                     LRERLEIEALLVTRSEEGMSLFLKEQVLHVPAVAKEVFDVSGAGDTVIATIGVVLATG
                     APLAEAVRWANKAAGIVVSKLGTAVVRPDELFS"
     gene            complement(3024..4355)
                     /locus_tag="VD791_10210"
     CDS             complement(3024..4355)
                     /locus_tag="VD791_10210"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HXV08391.1"
                     /translation="MKITMIGSGYVGLVTGACFADTGNEVLCLDLDARKIAMLNAGRI
                     PIYEPGLEAIVARNVNAGRLRFTTDVAESVDFGTVQIIAVGTPPDGDGAADLRNVVAA
                     ARNIGRHMRAYRLIVDKSTVPVGTADMVRSAVAEELAARGIEVPYSVVSNPEFLKEGA
                     AVEDFMKPDRVVVGSDDERATQFMRALYAPFQRSHERMLVMDVRSAELTKYAANAMLA
                     TRISFMNELANLAERLGADIEQVRRGIGSDPRIGYHFLYAGCGYGGSCFPKDVQALQR
                     TAADNGMQLRVLQAVEQVNHDQKRLLLAKVVRRFGERLDGRRFALWGLAFKANTDDMR
                     EAPSRAVIDGLLERGARVHAYDPAAEQEARRLYAGQPGLDYAATPMAALDGADALIIV
                     TEWKEFRSPDFEEIRTRLREPVLFDGRNLYDPALVRSAHLEYHGVGRGTGV"
     gene            complement(4352..5098)
                     /gene="pyrF"
                     /locus_tag="VD791_10215"
     CDS             complement(4352..5098)
                     /gene="pyrF"
                     /locus_tag="VD791_10215"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HXV08392.1"
                     /translation="MSYPARGPDLGPRAIVALDYADAGSALALSARLSPERCRLKVGK
                     ELFTAAGPALVENLAQRGFDVFLDLKYHDIPNTVAQACRAAAALGVWMLDVHALGGGA
                     MMRAAREALGTARRPHLIAVTVLTSHVQSDLDEIGIQGSPQSAARRLAALARRCGLDG
                     VVCSAQEAPALRAEQGPGFLLVTPGIRMAGEAGDDQKRITTPRAAVESGASYLVVGRP
                     VTRASDPLAALSAINREIERAAAGGRSEDA"
     gene            complement(5095..6270)
                     /gene="lapB"
                     /locus_tag="VD791_10220"
     CDS             complement(5095..6270)
                     /gene="lapB"
                     /locus_tag="VD791_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HXV08393.1"
                     /translation="MEFELWWLLGFPLFFALGWLAARVDIKQLLTESRALPSSYFKGL
                     NFLINQQPDKAIEAFIEVVKVDPQTVELHFALGSLFRRRGEVERAIRMHQNLVDRADL
                     GNEQRVTALYELAQDYLKAGLLDRAEELFLKLEGTQYEQQATRFLLEIYEQEKDWHKA
                     IQTSRRMDSIAGHSSAHEIAHFYCELAQAEIVHLRGEQARRYLDEALSVNRKCMRAYI
                     LQGDLAMAEGDPERAIEAWKRIEAQNAAYLPLVADKLLAAFNQIGRGEEGLALLRGYL
                     AGNPSLDLLNTVFQAVLERQGPAAANELVRDELRRYPTLLGLDRLLEAQAMEAPPERR
                     RDLELIRDLVHQHTRSLAMYRCESCGFKARQFYWHCPACHGWETYPPKRSEERELAA"
     gene            complement(6275..6559)
                     /locus_tag="VD791_10225"
     CDS             complement(6275..6559)
                     /locus_tag="VD791_10225"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HXV08394.1"
                     /translation="MRYLSWIAKLLLFLLLLGFALKNTEPVALRYFLGQEWRAPLSLV
                     LLLFFGAGAALGVAAALGVAWRLRRELDAQRRGAVGAPRGRAEPTDVTAV"
     gene            complement(6708..7010)
                     /locus_tag="VD791_10230"
     CDS             complement(6708..7010)
                     /locus_tag="VD791_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HXV08395.1"
                     /translation="MTKSELISRLAAHFPQLVASDAELAVKMILDAMSKSLSDGQRIE
                     IRGFGSFGLNYRPPRVGRNPKSGEKVQVPAKYVPHFKAGKELRERVDAKRSDPDRS"
     gene            complement(7170..8891)
                     /gene="rpsA"
                     /locus_tag="VD791_10235"
     CDS             complement(7170..8891)
                     /gene="rpsA"
                     /locus_tag="VD791_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HXV08396.1"
                     /translation="MTTATPAQASDKTENFAAMFEESLSRKEMRAGEVITAEVVRVDA
                     NFAVVNAGLKSESYIPIEEFKSDRGELEVKPGDFVQVAIEALEDGHGETRLSREKAKR
                     LAAWTELEQALEQGTVVQGLVNGKVKGGLTVMINGIRAFLPGSLVDLRPVKDTNPYEG
                     KQLEFKVIKLDRKRNNVVVSRRAVLEASAGEERQKLLEALQEGAVVKGIVKNITDYGA
                     FVDLGGIDGLLHITDLAWRRVKHPSEVLSVGDEVTAKVLKFDQEKNRVSLGLKQLGED
                     PWVGIARRYPTGTRLFGKVSNLTDYGAFVEIESGIEGLVHVSEMDWTNKNVHPSRVVQ
                     VGDEVEVMILEIDEERRRISLGMKQCMPNPWEEFAGSFKKGDRVKGQIKSITDFGVFI
                     GLPGGIDGLVHLSDLSWSMPGEEAVRQYKKGDEIEAVVVAIDVERERISLGIKQLEGD
                     PFTNYIARHEKGSLVEGKVKSVDAKGAVVTLQGDVEGYLRASEVSRDRVEDIRTHLKE
                     GDIVNAMIINIDRKNRGINLSIKQKAMAEETEALQKHAQDSVPGAGTTNLGALLKAKL
                     DTKNTAQ"
     gene            complement(8970..11000)
                     /locus_tag="VD791_10240"
     CDS             complement(8970..11000)
                     /locus_tag="VD791_10240"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HXV08397.1"
                     /translation="MRSFPPHLDLPPLAAARGGVRLPGSKSISNRSLLLAALAEGVTE
                     IRDLLAADDVERMLEALVSLGVRVEHAGERVRVHGCSGPFPVKQADLFLGNAGTAFRP
                     LTAVLALCGGHYRLSGVPRMHERPIGDLVEGLRQLGADVRYLAAAGFPPLEVWPAQIR
                     LEGPLQVRGDVSSQFVSALLMALPLLGRRAVVEVVGELVSRPYVEITLDLMARFGVRV
                     EREDRVAGDAGRFTEDTGGPRFIVPAGQHYVSPGVLHVEGDASSASYFLAAGAIGGGP
                     VRVQGVGRGSIQGDVRFADALAAMGARIASGENWIEAAAPAQGGLRAFDLDLNHIPDA
                     AMTLAVAALFADGPCRLRNIASWRVKETDRLAAMAAELRKVGAEVEEGTDSLTVSPPR
                     AARLVPRASIDTYDDHRMAMCFALVALGGVPVRINDPKCVAKTFPDFFERLAAITRPV
                     PVIALDGPSASGKGTVARGVARALGFEFLDSGALYRLVALAALQAGIDPGDEARVGRI
                     ARDLDVGFEDETIRLARQDVTDAIRREDVSVAASRVAALPAVRAALLERQRDFRRPPG
                     LVADGRDMGSTVFPDAVLKVFLTASPGERARRRHKQLMEKGIDATLPSLLQDIEQRDA
                     RDVERGVSPLVRCEDARLLDTTGLGAEQAIQQVLHWYREAANGQDRPAERRR"
     gene            complement(11004..11882)
                     /locus_tag="VD791_10245"
     CDS             complement(11004..11882)
                     /locus_tag="VD791_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HXV08398.1"
                     /translation="MPALTLHKLAVFGVGLIGGSFAAALKSAGLVCRVAGVGRSRDNL
                     ERAAALGLIDEIAQDAAAALDGADLALVAVPVRQTEAVLEQIAPHLPPACVVTDAGST
                     KQDVVAAARRALGPRIAQFVPAHPIAGAELSGAQAASATLYRDRLVVLTPLPETEPRA
                     CAAVEALWRACGARPVQMTPQRHDQIFAAVSHLPHALAFALVHMLAERTDAQELFAFA
                     GAGFRDFTRIAGSSPEMWRDICLANRDALGAEIAAFQQALAALAAHLEQRDAQAIERL
                     FEAARAARSPRLPGVE"
     gene            complement(11891..>12032)
                     /locus_tag="VD791_10250"
     CDS             complement(11891..>12032)
                     /locus_tag="VD791_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HXV08399.1"
                     /translation="YQRLLAQGVIVRPVAAYGMPEHLRVTVGLESENARFLAALRTAL
                     HA"
BASE COUNT         1785 a         4267 c         4067 g         1913 t
ORIGIN      
        1 cccgagcgcc tgcggcaaga ccaacttcgc gatgctgatc cccccgcccg ctttcggcgg
       61 ctggaaagtg accaccatcg gcgacgacat cgcgtggatc aagcccggtg cggacggccg
      121 gctgtacgcc atcaatccgg agttcggctt cttcggcgtc gcacccggca cttcccccaa
      181 gtccaatccg aacgcgctcg cgacgctggc gcgcaactgc gtgttcacca acgtggcgct
      241 caccgaggac ggcgacgtgt ggtgggagga cctgaccgag cagccgccgg cgcgtctggt
      301 cgactggcag ggccagccgt ggacgccgga ttgcgggcgc accgcggcac accccaacgc
      361 acgcttcacc gtgcccgcgg cgcaatgccc ggtcatcgat ccggattggg agaagcccga
      421 gggcgtgccg atctccgcgt tcatcttcgg cggccgacgc agccggtcga tcccgctggt
      481 atacgagacc tttcactggg tggacggcgt gtacgccgct gccacgatgg gctcggagac
      541 caccgcggcg gccgcaggca aggtgggcga ggtgcgccgc gatcccttcg ccatgctgcc
      601 gttctgcggc tatcacatcg gcgactactt cgatcactgg ctggagatcg gccggcgcgt
      661 tcccaacccg ccgcgcgtct tctgcgtgaa ctggttccgc cgcggcgacg acggcgcctt
      721 cctgtggccc ggctacggcg agaacatgcg tgtgctgaag tggatcgtgg accgctgccg
      781 gggacgcgcc ggggcgaccc agagcgtgct gggctggatg ccgcgcgagc atgacctgga
      841 gtgggccggc ctggatgcgg gcgttcgacg ccgctatccg gagctgatgt cgatcgagcg
      901 cgacgcgtgg atccacgagc tgttgatgca cgaggagctg tttgtgaggt tgtacgacaa
      961 gctgcccaag gagttcgtgc tcaagcgcga gctgctgctg gcgagcctgt ggcgctcgcc
     1021 ggaacgctgg acggtgccgt agctccgatt tattgtttcg cgggagcggt ggcggccgcc
     1081 tcccgtcgct actgcttcag caaccagtcg acgtagcgac gcacgccctg ttcgacgctc
     1141 aagaacggtg cgctgtagcc ggcctggcgc aactgcgtga ggtcggcctg ggtgaagctc
     1201 tgatacctgt cgggcaggcc ggccgggaac gggatgtagt cgatgacgtt ctgcgccagc
     1261 atctcctcca gcggcagagc gtccttgccc tgcgacgcgc gcaccgcatt gatcgtggcc
     1321 accgccagct cgttgaaggt ctgcgcccgg ccggtgccca cgttgaagat gcccgagcgg
     1381 ccgggatgat cgaggaagaa caagttcacc gcgaccacgt cctcgacgaa gacgaagtcg
     1441 cgccgctgct cgccggcggc gtacccgccg ctgccctcga acagctgcac ccgaccctgc
     1501 gtccgatact gattgaagaa atgccaggcg accgaagcca tgcgcgcctt gtgcgcttcg
     1561 cgcgggccgt agacgtcgaa atagcgcaat cccacggcct gggcgctgct ttcgcccaag
     1621 cgccgtcgca ccacctggtc gagcagcagc ttcgagtagc cgtagacgtt gagcggcttc
     1681 tcgaattgcg cgtcctcccg gaaggcgggg ccggcgccgt acaccgccgc cgacgacgca
     1741 tacaggaacg gaatgtcttc ggaccggcag aactcgagca gctccagcga acagcggtag
     1801 ttgttgtcga gcagatagcg cccgtccgcc tccagggtgt cggagcaggc gccctgatgc
     1861 agcacggcgc tcacttcgcc gtcgaagtcg ccggcggcga gcgcttggcg gaaggcgtcc
     1921 ttgtccaggt agtcggcgag ctcgcagtcg acgagattgc ggaacttgtc gccgcgcgcg
     1981 aggttgtcca ccgcgacgat atcggtgtcg ccgcggcggt tgagcgcttt caccaggttg
     2041 gatccgacaa agccggctgc gccggtgacc acgatcattc gagcagtcct ccgctcagga
     2101 aaacagctcg tcggggcgga ccaccgctgt gcccagcttg ctcaccacga tgccagccgc
     2161 cttgttggcc cagcgcaccg cctcggccag cggtgcgccg gtcgccagaa ccacgccgat
     2221 cgtggcgatc acggtgtcgc ctgcgccgct gacgtcgaac acttccttcg ccaccgccgg
     2281 cacgtgcaga acctgttcct tcaggaacag gctcatgccc tcctcgctgc gtgtcaccag
     2341 cagggcctcg atctccaggc gctcgcgcag ccgctgcccg cgcgcctcga aatccgcgtc
     2401 gctcttccag ctgccgacca ccgtgcggaa ttcgttgcgg ttgggggtca gcaacgtcgc
     2461 gtaccggtac ttgtcgtagt cgtcgccctt cggatcgatc agcaccggcc tgccggcggc
     2521 gcgcgtggcc tggatcatgc gctccacgtg tgtcagcgcg cccttggcgt agtcggacag
     2581 gatcaccacc tgggccagcg ggaggcgcga ctcgaagtcg gcgagcttgg cctccagcgc
     2641 gttggccgca ggaagattct cgaaatcgat gcgcaccagc tgctgctgcc gcccgaccac
     2701 ccgcagcttg acggtggtgc tcagcgtcgg gtcgcgggtc agatgcacgt gcaccccctc
     2761 cgcggcgagc agccgctcca ggctcgcacc cggctcgtcc tccccgatca ccgccaggag
     2821 ctgcacctgc gcgcccagtg cggtggcgtt gcgcgccacg ttggcagcgc cgcccgggcg
     2881 ctcctccatg cggccgatgc gcaccaccgg caccggggcc tcgggcgaga tgcggctcac
     2941 ctcgccgaac cagtactggt cgagcatgac gtcgcccacc accagcaggc gggccttgct
     3001 cagcagggcg cgatcgatgc tcatcacacg ccggttccac gtcccacgcc gtggtattcg
     3061 agatgggcgc tgcgcaccag cgccggatcg tacagattgc ggccgtcgaa cagcaccggc
     3121 tcgcgcaacc gggtacggat ctcctcgaag tcggggctgc ggaactcctt ccactcggtg
     3181 acgatgatca aggcatctgc gccgtccagc gccgccatcg gcgtcgcggc gtaatccagc
     3241 cccggctgac cggcgtacag gcggcgcgcc tcctgctccg cggccgggtc gtaggcgtgc
     3301 acgcgcgcgc cgcgctcgag caggccgtcg atcaccgccc ggctgggcgc ctcgcgcatg
     3361 tcgtcggtat tggccttgaa ggccagcccc cagagcgcga agcggcggcc gtccagccgc
     3421 tcgccgaacc ggcgcaccac cttggcgagc agcagccgct tctggtcgtg attgacctgc
     3481 tccaccgcct gcagcacgcg cagctgcatg ccgttgtccg cggccgtgcg ctgcaaggcc
     3541 tgcacgtcct tggggaagca cgatccgccg tagccgcagc cggcgtacag gaagtggtag
     3601 ccgatgcgcg gatccgagcc gatgccgcgg cgcacctgct cgatgtcggc gcccagccgt
     3661 tcggccagat tggccagctc gttcatgaac gagatgcgcg tggcgagcat ggcgttggcg
     3721 gcgtacttgg tgagctcggc ggatctcacg tccatcacca gcatgcgctc gtggctgcgc
     3781 tggaacgggg cgtacagcgc gcgcatgaat tgggtggcgc gctcgtcgtc gctgcccacc
     3841 accacccggt cgggcttcat gaagtcctcg accgcggcgc cctccttgag gaactcgggg
     3901 ttcgacacca cgctgtaggg cacctcgata ccgcgcgcag ccagctcttc ggccacggcg
     3961 ctgcgcacca tgtcggcagt gccgaccggc accgtggact tgtccacgat cagccgatag
     4021 gcgcgcatgt ggcggccgat gttgcgcgcc gcggcgacca cgttgcgcag atcggccgcg
     4081 ccgtcgccgt ccggcggcgt gcccaccgcg atgatctgca cggtgccgaa gtcgacgctc
     4141 tcggccacgt cggtggtgaa gcgcagcctg ccggcgttca cgttgcgggc gacgatggcc
     4201 tccagccccg gctcgtagat cgggatgcga cccgcgttga gcatggcgat cttgcgcgcg
     4261 tcgaggtcca ggcacagcac ctcgttgccg gtgtccgcga agcaggcgcc ggtgaccagg
     4321 cccacgtagc cgctgccgat catggtgatc ttcacgcgtc ctccgaccgg ccgccggcgg
     4381 cggcccgctc gatttcccgg ttgattgccg acagcgcggc gagcggatcg ctggcccggg
     4441 tcaccggccg gcccaccacc agatagctgg cgccgctctc gacggccgcc cgcggcgtgg
     4501 tgatgcgctt ctggtcgtcg ccggcctcgc ccgccatgcg gatgcccggg gtcaccagca
     4561 ggaagcccgg cccctgctcc gcccgcagcg ccggcgcctc ctgcgcggag cacaccacgc
     4621 cgtccagccc gcacctgcgc gcaagggcgg caaggcgtcg cgcggccgac tgcggcgacc
     4681 cctggatgcc gatctcgtcg aggtcgctct gcacgtggct ggtgagcacg gtcaccgcga
     4741 tcaggtgcgg acggcgagca gtacccaggg cctcgcgcgc cgcgcgcatc atcgcccccc
     4801 cgcccagagc gtgcacgtcg agcatccaca cgccgagcgc ggccgccgcg cggcaggcct
     4861 gcgccacggt gttggggatg tcgtggtact tgagatccag gaacacatcg aaaccgcgtt
     4921 gcgccaggtt ctcgaccaag gccggtccgg ctgcggtgaa caactctttt ccgaccttca
     4981 ggcggcaacg ctccggcgac agccgcgcgc tgagcgccag cgctgagccc gcgtccgcat
     5041 agtcgagcgc cacgatcgca cgcggcccga ggtcgggtcc cctcgccggg tagctcatgc
     5101 ggccaattcc cgttcttcgc tgcgcttggg cggataggtt tcccagccgt ggcaggcggg
     5161 gcaatgccag tagaactggc gggccttgaa tccgcagctc tcgcaacggt acatggcgag
     5221 gctgcgcgtg tgctggtgca ccaggtcgcg gatcagctcg aggtcgcggc ggcgctcggg
     5281 cggcgcctcc atcgcctgcg cctccagcaa ccggtccagg cccagcagcg tggggtagcg
     5341 ccgcaactcg tcgcgcacca gctcgttggc cgcggccggg ccctggcgtt cgagcaccgc
     5401 ctggaacacg gtgttcaaca gatccagcga tgggttgccg gcgaggtagc cgcgcagcag
     5461 ggccaggccc tcttcgccac ggccgatctg gttgaaggcg gccagcagct tgtccgccac
     5521 cagcggcagg taggcggcgt tctgggcttc gatgcgcttc caggcctcga tcgcccgctc
     5581 cgggtcgccc tccgccatgg ccaggtcgcc ctgcaggatg taggcgcgca tgcacttgcg
     5641 gttgaccgac agcgcttcgt ccaggtagcg gcgcgcctgc tcgccgcgca ggtgcacgat
     5701 ctcggcctgc gccagctcgc agtagaagtg tgcgatctcg tgtgcgctcg agtgcccggc
     5761 gatgctgtcc atgcggcgcg aggtctggat cgccttgtgc cagtccttct cctgctcgta
     5821 gatctcgagc aaaaagcgcg tggcctgctg ctcgtattgc gtaccctcga gcttgagaaa
     5881 cagctcttcg gcgcgatcca gcaacccggc cttgaggtag tcctgtgcga gctcgtacag
     5941 cgcggtcaca cgctgctcgt tgcccaggtc ggcgcgatcc accaggttct ggtgcatgcg
     6001 aatggcgcgc tcgacctcgc cgcggcggcg gaacaggctg cccagcgcga agtgcagctc
     6061 gacggtctgc gggtcgacct tgaccacctc gatgaacgcc tcgatggcct tgtcgggctg
     6121 ctggttgatg aggaagttga gccctttgaa gtacgagctg ggcagcgcgc gcgactcggt
     6181 gagcagctgc ttgatgtcga cgcgggcggc gagccagccg agcgcgaaga acaaggggaa
     6241 acccagcagc caccagagct cgaattccat ggcgtcacac ggcggtgacg tcggtgggct
     6301 cggcgcgacc cctgggcgcg ccgaccgcac cgcgccgctg cgcatccagc tcgcggcgca
     6361 gccgccaggc gacgccgaga gcggccgcca cgccgagcgc cgctccggca ccgaagaaaa
     6421 gcagcagcac caggctcagc ggcgcgcgcc actcctgccc caggaagtag cgcagcgcca
     6481 ccggctcggt gttcttgagg gcgaagccga gcaggagcag gaacagcagc agcttcgcga
     6541 tccagctcag gtagcgcatg ggtcaaccgt gcaggcgcac ggacgcagag aaaaccgcaa
     6601 cgagcagggc gatgccggga tgatcgcgcg gttccggggc ccggcacaag gctgcgatgg
     6661 ctcggtgcct ctgcgtccct gtgccgagaa accagccccg gcgcttacta tgacctgtcc
     6721 gggtcggacc gcttggcatc cacgcgctcg cgcagctcct tgccggcctt gaagtgaggc
     6781 acgtacttgg ccggcacctg gaccttctcg ccggacttgg ggttgcgccc cacgcgcggc
     6841 ggccggtaat tcagcccgaa gctgccgaat ccccggatct cgatgcgctg cccatcggac
     6901 aggctcttcg acatggcgtc gaggatcatc ttcaccgcca gctcggcgtc gctggccacc
     6961 agctgcggaa agtgcgccgc cagcctggag atcaactccg acttcgtcat ggctaccctg
     7021 tcagttaccg gcggctttgt tatcggaacc cgcgccctgt tcttgaaaac ggcggccgca
     7081 cgcgcgcgcg acctgccttg gaactgcggc tgtcgtcggc ggcgtaccgg ccgcccccgg
     7141 tgcagcgtcc tacgctctct gccgtggcct cactgagcgg tgttcttggt gtcgagtttg
     7201 gccttgagca acgcgcccag gttggtggtg ccggcgcccg ggacgctgtc ctgggcatgc
     7261 ttctgcagcg cctcggtctc ctccgccatc gccttctgct tgatggacag gttgatgccg
     7321 cggttcttgc ggtcgatgtt gatgatcatc gcgttcacga tgtcaccttc cttcaagtgg
     7381 gtgcggatgt cctcgacgcg gtcgcgcgac acttccgagg cgcgcaggta gccttcgacg
     7441 tcgccctgca gtgtcaccac cgcgcccttg gcgtccaccg acttgacttt gccctcgacc
     7501 aggctgccct tctcgtgtcg ggcgatgtag ttggtaaacg ggtcgccctc gagctgcttg
     7561 atgccgagcg agatgcgctc gcgctccacg tcgatggcca ccaccacggc ctcgatctcg
     7621 tctcccttct tgtactggcg caccgcctcc tcgcccggca tgctccagga caggtcggac
     7681 aggtgcacca ggccgtcgat cccgcccggc aggccgatga acacgccgaa atcggtgatc
     7741 gacttgatct ggcccttgac ccggtcgccc ttcttgaaac tgccggcgaa ctcctcccac
     7801 gggttgggca tgcactgctt catgccgagc gagatgcggc ggcgttcctc gtcgatctcg
     7861 aggatcatca cttcgacctc gtcgccgacc tgcaccacgc gcgagggatg cacgttcttg
     7921 ttggtccagt ccatctcgga tacgtgcacc aggccctcga tgcccgactc gatctcgaca
     7981 aacgcgccgt agtcggtgag gttgctgacc ttgccgaaca ggcgcgtgcc ggtcggatag
     8041 cggcgcgcga tgcccaccca ggggtcttcg ccgagctgct tgagccccag cgacacgcgg
     8101 ttcttctcct ggtcgaactt gagcaccttg gcggtgacct cgtcgcccac ggacagcact
     8161 tccgaagggt gcttgacgcg gcgccaggcg aggtcggtga tgtgcagcag gccgtcgatg
     8221 ccgcccaggt ccacgaacgc gccgtagtcg gtgatgttct tgacgatgcc cttgaccacc
     8281 gcgccttcct gcagcgcttc cagcagcttc tggcgctcct cgccggcgga ggcctcgagc
     8341 accgcgcggc gcgacaccac cacgttgttg cgcttgcggt cgagcttgat caccttgaat
     8401 tcgagctgct tgccctcgta ggggttggtg tccttcaccg ggcgcaggtc caccagcgag
     8461 ccgggcagga acgcgcggat gccgttgatc atgacggtca ggccgccctt gaccttgccg
     8521 ttgaccagcc cctgcaccac cgtgccctgc tccagggcct gctccagctc ggtccaggcg
     8581 gccaggcgct tggccttctc gcgcgacagg cgcgtctcgc cgtggccgtc ttccagcgcc
     8641 tcgatcgcca cctgcacgaa gtccccgggc ttgacctcca gctcgccgcg atcgctcttg
     8701 aactcctcga tcgggatgta gctctccgat ttgaggccgg cgttgaccac cgcgaaattg
     8761 gcgtccacgc ggaccacttc ggcggtgatc acttcgcccg cgcgcatctc cttgcgcgac
     8821 agactttcct cgaacatggc ggcgaagttc tcggttttgt cggaggcttg ggctggggtg
     8881 gcggttgtca tcagaaaacg ttgtttcctg ctaaaaaccc atcgcgggga tggggccggt
     8941 tgatcaaatg gaccgcggca cgcgacgctc tagcggcgcc gctcggccgg ccggtcctgt
     9001 ccgttggctg cctcccgata ccaatgcagc acctgctgga tcgcctgttc ggcgcccagg
     9061 ccggtggtat caaggagccg tgcatcttcg cagcgcacaa gcggggagac gccgcgctcg
     9121 acgtcgcgcg catcgcgctg ctcgatgtcc tgcaaaaggg aagggagtgt agcatcgatt
     9181 cctttttcca tcaactgttt atgccggcgc ctggcgcgct ccccggggct cgccgtgaga
     9241 aacaccttga gcacggcgtc ggggaacacg gtcgagccca tgtcgcgccc gtcggccacc
     9301 aggccgggcg gccgccggaa gtcgcgctgg cgctccagca gggcggcgcg caccgcgggc
     9361 agggccgcta cgcgggatgc ggccactgac acatcctcgc ggcggatggc gtcggtgacg
     9421 tcctgcctgg caaggcgaat ggtctcgtcc tcgaagccca cgtcgaggtc ccgggcgatc
     9481 cgccccaccc tggcctcgtc gccggggtcg atcccggcct gcagcgccgc cagcgccacc
     9541 agccggtaca gagcgccgct gtccaggaat tcgaagccta gcgcccgggc aaccccccgg
     9601 gcgaccgtgc ccttgccgga ggcggacggc ccgtcgaggg cgatcaccgg caccggccgg
     9661 gtgatcgcgg cgaggcgctc gaagaagtcg ggaaaggtct tcgccacgca tttcggatcg
     9721 ttgatccgca ccggcacccc gcccagcgcg accagggcga agcacatcgc catgcgatgg
     9781 tcgtcgtagg tgtcgatcga ggcccgcggc acgaggcgcg cggcgcgcgg gggtgacacg
     9841 gtgagggaat ccgtgccctc ctccacttcg gcgccgacct tacgcagctc agccgccatc
     9901 gcggccagcc ggtcggtttc cttcacccgc cagctggcga tgttgcgcag ccggcagggg
     9961 ccgtcggcga acagcgcggc gaccgccagc gtcatggcgg cgtcgggaat gtggttgagg
    10021 tccaggtcga aggctcgcag ccccccttgt gccggtgccg ccgcctcgat ccagttctca
    10081 cccgacgcga tgcgcgcgcc catggccgcc agcgcatcgg cgaaccgcac atcgccctga
    10141 atgctgcccc gacccactcc ctgcacgcgc accgggccgc cgccgatcgc gccggcggcc
    10201 aggaagtagg acgcgctcga ggcgtcgccc tcgacgtgca gcacgccggg gctgacgtaa
    10261 tgctgcccgg cggggacgat gaagcgcggc cccccagtgt cctcggtaaa gcggccggcg
    10321 tccccggcaa cgcggtcctc gcgctcgacg cgcaccccga agcgcgccat caggtcgagc
    10381 gtgatctcca catagggcct ggagacgagc tcgcccacca cctccacgac cgcccgccgg
    10441 ccaagcagcg gtagcgccat cagcagcgcg ctcacgaact ggctggagac gtccccgcgc
    10501 acctgcagcg gcccttccag ccggatctgc gcgggccata cctcgagcgg cggaaagccc
    10561 gcagcggcga gatagcgcac gtccgcgccg agctgccgca ggccttcgac caagtcgccg
    10621 atcgggcgct cgtgcatgcg cggcacgccg ctcagccggt agtgtccgcc gcacaacgcc
    10681 agtacagcgg tgaggggacg gaacgccgtt ccggcattgc cgaggaacag gtcggcctgc
    10741 ttcaccggaa acggcccgct gcaaccgtgc acgcgcacgc gctcgccggc atgctccacc
    10801 cgcacgccca ggctgacgag cgcctccagc atgcgctcga catcgtcggc ggcgagcaga
    10861 tcgcggatct cggtcacgcc ttcggccagg gccgcgagca gcaggctgcg attggagatg
    10921 ctcttggagc cgggcaggcg cacgccgccg cgggcggcgg cgagcggcgg caggtcgaga
    10981 tgcggcggaa agctgcgcat gtttcactct acgcccggca gccgggggct gcgcgcggca
    11041 cgggcggcct cgaacaggcg ctcgatggct tgtgcgtcgc gctgctccag atgcgccgcc
    11101 agcgcagcca gggcctgctg gaaggccgcg atctccgcgc cgagcgcgtc gcggttggcc
    11161 aggcagatgt cgcgccacat ctccggcgag ctgccggcga tgcgggtgaa gtcgcggaaa
    11221 ccggctccgg cgaaggcgaa cagctcctgc gcgtcggtgc gttcggcgag catgtgcacc
    11281 agggcgaagg cgagcgcgtg cggcaggtgg ctcaccgccg cgaagatctg gtcgtggcgc
    11341 tgcggtgtca tctgcaccgg ccgcgccccg caagcgcgcc acagggcctc caccgcggcg
    11401 caggcgcgcg gctcggtctc cggcagcggc gtgagcacga ccaggcggtc gcggtacagc
    11461 gttgcgctgg ccgcctgcgc gcccgacagc tccgcgccgg cgatgggatg cgcgggcacg
    11521 aactgcgcga tgcgcggccc cagcgcgcga cgcgccgccg ccacaacgtc ctgcttggtg
    11581 ctgcctgcgt cggtgaccac gcaagccggc ggcaggtgcg gggcgatctg ctcgagcacc
    11641 gcctcggtct gccgcaccgg cacggccacg agcgcgaggt cggccccgtc gagcgcggcg
    11701 gcggcatcct gggcgatctc gtcgatcagg ccgagcgcgg ccgcgcgctc gagattgtcc
    11761 cggctgcgcc ccacgccggc caccctgcac accaggccgg ccgatttcag cgccgccgcg
    11821 aacgaaccgc cgatgaggcc gacgccgaag acggcgagct tgtgcagcgt gagcgcgggc
    11881 aattggatgg tcaggcgtgc agcgcggtcc gcagggcggc caggaagcgg gcgttctcgg
    11941 actccaggcc gacagtgacg cgcaggtgct cgggcatacc gtaggccgcg accgggcgta
    12001 cgatcactcc ctgtgcaagc agccgctggt ac
//
LOCUS       DASPGE010000318         4243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1604836, whole genome shotgun sequence.
ACCESSION   DASPGE010000318 DASPGE010000000
VERSION     DASPGE010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604836"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1630
                     /locus_tag="VD791_10255"
     CDS             <1..1630
                     /locus_tag="VD791_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HXV08400.1"
                     /translation="LSTTLFAELRDIVFVRVAKRAIRRVALQVFRHLHALSLRFHLER
                     QTGGVSRDIERGTRGISTLLNYMLFSILPVILEFGLVAAVLLARFDWHFAAVTFAAVA
                     VYMAFTIRVTEWRMDFRRRSNELDSRANTRAIDSLLNYETVKYFNNEEWEARRYDDNL
                     RKFESAEVKNEASLGVLNIGQSAIIALAVTLLMILAAQGVVQRTLTLGDLVLVNGLLI
                     QLYIPLNFLGMVYREVKQSLTDMDRMFRLLDVGREVQDKPGAAELPAGEAAVRFEHVD
                     FSYEVRRQILFGVSFEIPAGRTLAVVGHSGSGKSTLSRLLFRFYDVSAGRIAINGVDI
                     REVTQRSLRAAIGIVPQDTVLFNDTVYYNIAYGDPEATRAQVIAAARAAHIHDFIESL
                     PDGYEAMVGERGLKLSGGEKQRVAIARAILKNPRIFIFDEATSALDSKAEKAIQAELD
                     RIARGRTTLIIAHRLSTVVDADQILVMDHGRIVERGTHRELLAVGGAYAQMWALQQRE
                     RAEREAEEVPEEEEPAEHAAPAQAPLQAGAHARS"
     gene            2071..2982
                     /gene="pbpG"
                     /locus_tag="VD791_10260"
     CDS             2071..2982
                     /gene="pbpG"
                     /locus_tag="VD791_10260"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF008668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HXV08401.1"
                     /translation="MSDPVVVTGLPVLEPLPAPLELRVDPAQRQRLTISDSGDAAGPE
                     DLALRSAAALVISQDDGQLLYAKNTDAVMPIASITKLMTAMVILDSGLSLDEPVTITR
                     HDVDRLKGTKSRLQLGLTLPRREMLRLALMASENRAAAALARSHPGGTEAFVQAMNLK
                     ARQLGMRDTRFVDASGLNPNNVSTALDLAVLVNAGYHYPLIRDYSTTDSVRLAFRGSR
                     KTRAMAFRNSNSLVRSAQWEIGLQKTGYISEAGRCLVMQARISARPVIIVLLDSWGKF
                     TRVGDANRIKRWVETLDIPAAHKPRLM"
     gene            3107..3841
                     /locus_tag="VD791_10265"
     CDS             3107..3841
                     /locus_tag="VD791_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXV08402.1"
                     /translation="MRVGLFVTCLVDLMRPRIGLAALELLEGAGCEVLVPAQQTCCGQ
                     PGYNSGDRRAAQALARKLLREFKDVDYVVVPSGSCAGTIKTHFPELLSEFPELRAEVE
                     WLAARTWELTDFLVNVLEIERVPGRFRGAVTYHDSCSGLREMGVKAQPRTLLALVPGL
                     VLREMTAPEQCCGFGGTFAVKFGAISARICDQKCDDVQASAADAVVLGDLGCMLNIEG
                     RLRRRGNRRTRVLHVAEVLVGGADEG"
     gene            3845..>4243
                     /locus_tag="VD791_10270"
     CDS             3845..>4243
                     /locus_tag="VD791_10270"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HXV08403.1"
                     /translation="MQVRSQEFKARAARSLSDPHLAKALANLRQRMVLGRIASVAELD
                     NFEQAREAAKRVRDDALQHLDLLLEQFERNATARGTRVHWAETPQDMNRIVLDIAQRA
                     GVRKAIKSKSMLGEESGLNAYLAAAGIEVRE"
BASE COUNT          657 a         1459 c         1459 g          668 t
ORIGIN      
        1 cctgtccacc acgctgttcg ccgagctgcg cgacatcgtg ttcgtgcgcg tggccaagcg
       61 cgcgatccgg cgcgtggcgc tgcaggtgtt ccggcatctg cacgcgctca gcctgcgctt
      121 ccatctcgag cggcagaccg gcggcgtgtc gcgcgacatc gagcgcggca cgcgcggcat
      181 ctccacgctg ctcaactaca tgctgttctc catcctgccg gtgatcctgg agttcggcct
      241 ggtggcggcg gtgctgctgg cgcgcttcga ctggcacttc gccgccgtca ccttcgcggc
      301 ggtggcggtg tatatggcgt tcaccatccg cgtgaccgag tggcgcatgg acttccggcg
      361 ccgctccaac gagctcgatt cccgggccaa cacgcgcgcc atcgacagcc tgctcaacta
      421 cgagacggtc aagtacttca acaacgagga gtgggaggcg cgccgctacg acgacaacct
      481 gcgcaagttc gagtcggccg aggtcaagaa cgaagcctcc ctgggcgtgc tcaacatcgg
      541 ccagagcgcg atcatcgcgc tggcggtgac gctgctgatg atcctggccg cgcagggcgt
      601 ggtgcagcgc acgcttacgc tcggcgacct ggtgctggtg aacgggctgc tgatccagct
      661 ctacattccg ctcaacttcc tgggcatggt gtaccgggaa gtgaagcagt cgctgaccga
      721 catggaccgc atgttccgcc tgctcgatgt cggccgcgag gtgcaggaca agccgggcgc
      781 ggccgagctg ccggccggcg aggcggcggt gcgcttcgag cacgtggact tcagctacga
      841 ggtgaggcgc cagatcctgt tcggcgtgag cttcgagatc cccgccggca ggacgctcgc
      901 ggtggtcggg cacagcggct ccggcaagtc cacgctgtcg cggctcctgt tccgcttcta
      961 cgatgtgagc gccgggcgca tcgccatcaa cggggtggac atccgcgagg tcacgcagcg
     1021 cagcctgcgc gcggcgatcg gcatcgtccc gcaggacacg gtgctgttca acgacaccgt
     1081 ttattacaac atcgcgtacg gcgaccccga agccacgcgc gcgcaggtga tcgccgccgc
     1141 gcgcgcggcg cacatccacg acttcatcga gagcctgccc gacggctacg aggcgatggt
     1201 gggcgagcgc gggctcaagc tttccggcgg cgagaagcag cgcgtggcca tcgcccgggc
     1261 aatcctcaag aacccgcgca tcttcatctt cgacgaggcc acctcggcgc tcgactccaa
     1321 ggcggagaag gcgatccagg ccgagctcga ccgcatcgcg cgcgggcgca ccaccctgat
     1381 catcgcgcac cgcctgtcca ccgtcgtgga tgcggaccag atcctggtca tggaccacgg
     1441 ccggatcgtc gagcgcggca cccaccgcga gctgctcgcg gtcggcggcg cgtacgcgca
     1501 gatgtgggcc ctgcagcagc gcgagcgagc cgagcgcgaa gcggaggagg tgccggagga
     1561 ggaggagccc gcggagcacg ctgcccccgc ccaggcgccg ctgcaggccg gggcgcacgc
     1621 ccgcagttag gcgcaccggg cagggccgcg ggggttcgtc cagcgcgccc gatccggtat
     1681 cctgcatgga ggagacagcg cgacggcgcg ggacaatcgt caacaatccg ttgatcgcgg
     1741 acaccgtgca tgtgcatggc cgcgctgctt gcacggtgcg ctgcgcccgc gagctggcat
     1801 gcaccgtgcg gtgaggttcg aacgggcttt aacaaccttg cattagataa tgatttttgg
     1861 tagctttctg ccattgcgac cggcatcgga acggtctgtg cttaaagttc ccgcggccgc
     1921 cgtcgttacc tggcaggccg gcgccgggcg gatcagggaa ggcatggcat cgcccgcagc
     1981 gcactacgag attcgaatca cgaggttctg atgcacataa gaactgccct cgtcgttgtt
     2041 gcttcgctcc ccgcgctggc gtgggcgcag atgtccgacc cggtcgtggt gaccgggctg
     2101 cctgtcctgg agccgctgcc ggcccccctc gagttgcggg tcgatccggc gcagcggcag
     2161 cggctgacca tctcggattc gggcgatgcc gccggccccg aggatctggc gctgcgctcc
     2221 gcggccgccc tggtgatcag ccaggatgac ggccagctct tgtacgccaa gaacaccgac
     2281 gcggtgatgc cgatcgcgtc catcaccaag ctcatgacgg cgatggtgat cctcgactcg
     2341 gggctgtcgc tggacgagcc ggtcaccatc acccgccacg acgtcgaccg gctcaagggc
     2401 accaaatcgc gcctgcagct gggcctgacg ctgccgcggc gcgagatgct gcgcctcgcc
     2461 ctgatggcct ccgagaaccg cgcggcggcc gctctggcgc gcagccaccc gggtggcacc
     2521 gaggccttcg tgcaggcgat gaacctcaag gccagacagc tcggcatgcg cgacacgcgc
     2581 ttcgtggacg ccagcgggct caatcccaac aacgtctcca ccgcgctcga cctggccgtg
     2641 ctggtcaacg ccggctatca ctatccgctg atccgcgact actcgaccac cgactcggtg
     2701 cgcctcgcgt tccgcggcag ccgcaagaca cgcgcgatgg cgttccgcaa ttccaactcc
     2761 ctggtgcgca gcgcacagtg ggagatcggg ttgcagaaga cgggttacat cagcgaggcc
     2821 ggccgctgcc tggtgatgca ggcgcgcatc tccgcccggc cggtgatcat cgtgctgctc
     2881 gactcctggg gcaagttcac ccgcgtgggc gatgccaacc gcatcaagcg ctgggtcgag
     2941 acgctggaca tccccgcagc gcacaagccg cgcctgatgt aagcggcagg cgtccgccga
     3001 gcacgagccc cgccgcgagc ggggttcgtc gtttctgcgg gccgcgcgcc ggacacgcgt
     3061 gctatcatcc cgcgcctcgt cccgcgggaa gtcagcactt cgaccgatgc gcgtcggcct
     3121 gttcgtcacc tgtctggtgg acctgatgcg cccgcgcatc ggactggcgg ctctcgagtt
     3181 gctcgaaggc gccggctgcg aggtgctggt gcccgcgcag cagacctgct gcgggcagcc
     3241 cggctacaac tcgggcgacc gccgcgccgc gcaggcgctg gcgcgcaagc tgctgcgcga
     3301 gttcaaggac gtcgactacg tggtggtgcc ctccggctcc tgtgccggca ccatcaagac
     3361 gcactttccc gagctgctgt ccgagttccc cgagctgcgc gccgaggtcg agtggctggc
     3421 cgctcgcacc tgggaactga ccgacttcct ggtcaacgtc ctcgagatcg agcgcgtgcc
     3481 cggccgcttc cgcggcgcag tcacctacca tgattcctgc tcgggactgc gcgagatggg
     3541 cgtgaaggcg cagccgcgca cactgctcgc gctggttccc ggcctggtgc tgcgcgaaat
     3601 gacggcgccg gagcagtgct gcggcttcgg cggcaccttc gccgtcaagt tcggcgccat
     3661 ctccgcacgc atctgcgatc agaagtgcga cgacgtgcag gccagcgctg ccgacgccgt
     3721 ggtgctgggg gatttgggct gcatgctcaa catcgaaggg cgcctgcgcc gccgtggcaa
     3781 ccgccgcacc agggtgctgc acgtggcgga ggttctggtc ggcggcgccg acgagggctg
     3841 accgatgcag gtgcggtcgc aggagttcaa ggcgcgcgcg gcccggtcgc tgtccgatcc
     3901 gcatctggcc aaggcgctcg ccaacctgcg ccagcgcatg gtgctggggc gcatcgcctc
     3961 ggtcgccgaa ctggacaact tcgagcaggc gcgcgaggcg gccaagcggg tgcgcgacga
     4021 tgcgctgcag cacctcgatc tgttgctgga gcagttcgag cgcaacgcca ccgcgcgcgg
     4081 cacgcgcgtg cactgggcgg aaacgccgca ggacatgaat cgcatcgtgc tggatatcgc
     4141 gcagcgcgcc ggggtgcgca aggcgatcaa gtcgaaatcg atgctgggcg aggagtccgg
     4201 gctcaacgcc tacctcgcgg ccgccggcat cgaggtgcgc gag
//
LOCUS       DASPGE010000319         2993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_551035, whole genome shotgun sequence.
ACCESSION   DASPGE010000319 DASPGE010000000
VERSION     DASPGE010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551035"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1327
                     /locus_tag="VD791_10275"
     CDS             191..1327
                     /locus_tag="VD791_10275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXV08404.1"
                     /translation="MHDILIIGGGPVGSALALALRDSNLDVMLLEARREAAGDRRSLA
                     LSYGTRLILERVGAWREVTPATPIESIHVSQRGGFGRALLTAAAARLPALGYVAGYGT
                     VRDALTRAVLRCDRLQVRPGARANAMHPEDGHVTVSYALDGQTCSASARLVAVADGGA
                     LTQPGAESKERWYGQTALVAEVRTDRAHGNRAFERFTAEGPLALLPAGDGFALIWTAA
                     PERAQSLQALEPRAFLDALQACFGERAGRFVSVSARVTHPLALRVADSAHAQRVVLLG
                     NAAQTLHPVAGQGFNLGLRDAWELARAALDAPQRIGERNFIARFLRSRRTDRRDTILL
                     TDLLVNTFSNDLAPLRWLRGCGLTLLDCLPPAKRDFMGRMIFGA"
     gene            1425..2435
                     /gene="dusB"
                     /locus_tag="VD791_10280"
     CDS             1425..2435
                     /gene="dusB"
                     /locus_tag="VD791_10280"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HXV08405.1"
                     /translation="MQIGPHQLKNKLIVAPMAGVTDRPFRQLCKRLGAGMAVSEMLSS
                     RPELRHSAKSSRRADHAGEVEPIAVQIAGADPAMMAEAARYNVAQGAQLIDINMGCPA
                     KKVCNVMAGSALLRDEPLVGRILDAVVKAVDVPVTLKIRTGWDREHRNAVRVASIAES
                     AGVRALAIHGRTRACGFTGSAEYDTIAEVKSRTRLPVIANGDIATPEQAAHVLRHTGA
                     DSIMIGRAAQGRPWIFREIAAYLDTGRRPPGPGFGEIHALLREHLYALHAFYGETMGV
                     PIARKHAGWYMRALPGAAAWRARFNRIETSQSQLAEVDRYFEELAALDTGVGAQEEEL
                     AA"
     gene            2435..2668
                     /locus_tag="VD791_10285"
     CDS             2435..2668
                     /locus_tag="VD791_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXV08406.1"
                     /translation="MNENEIARCVRRTLDGYFRDLDGEKAAGVYNMVISCVERPLIEV
                     VLHRVGGNQTHAAELLGMNRNTLRKKMRTYGIK"
     gene            2753..>2993
                     /gene="purH"
                     /locus_tag="VD791_10290"
     CDS             2753..>2993
                     /gene="purH"
                     /locus_tag="VD791_10290"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617933.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HXV08407.1"
                     /translation="MAAARQALISVSDKTGVVELARGLVRLGFGILSTGGTARLLAEA
                     GVPVTEVGEYTGFPEILDGRVKTLHPKVHAGILARR"
BASE COUNT          462 a         1043 c         1046 g          442 t
ORIGIN      
        1 cgtgatcgaa gggcgtgacc gggaggcgtg accgggaagc gtggccaaga aggtgacggg
       61 taacggtcgc gcgaaacgct accgcttgca atgatggatc cgcacagtgg acgataactc
      121 ttaacgtatc gccgtcacgc ctttcggtca cgcagtaccg gtcacgctcc tcgatcacgc
      181 ctttcgcacc atgcacgaca tcctcatcat cggcggcggg ccggtcggct ccgccctcgc
      241 gctggcgctg cgcgacagca acctcgacgt catgctgctc gaggcgcgcc gcgaggccgc
      301 cggcgaccgg cgcagcctgg ccttgtccta cggcacccgc ctgatcctgg aacgcgtcgg
      361 cgcctggcgg gaggtgacgc cggcgacgcc gatcgagtcg atccatgtct cccagcgcgg
      421 cggcttcggg cgcgcgctgc tcaccgccgc cgccgcccgc ctccccgccc tgggctacgt
      481 cgccgggtac ggcaccgtgc gcgatgcact gacgcgcgcg gtgttgcgct gcgaccggct
      541 gcaggtgcga ccgggcgcgc gtgccaacgc aatgcatccc gaggacggcc acgtcacggt
      601 gagctacgcg ttggacgggc agacctgctc ggcttcggcg cgcctggtcg cagtggcgga
      661 cggcggcgcg ctcacgcagc cgggcgccga gtcgaaggaa cgctggtacg gtcagaccgc
      721 gctggtggcc gaagtgcgca ccgaccgcgc ccacggtaat cgcgcctttg agcgcttcac
      781 cgccgagggc ccgctggcat tgctgcccgc aggcgacggc ttcgccctga tctggaccgc
      841 ggcgcccgag cgcgcacaat cgcttcaggc cctggaaccg cgcgccttcc tggacgcgct
      901 gcaggcctgc ttcggggagc gtgccgggcg tttcgtgtcg gtgagcgcgc gagtgacgca
      961 cccgttggcg ctgcgggtgg ccgattccgc tcacgcccag cgcgtggtgc tgctgggcaa
     1021 tgccgcccag acgctgcatc cggtcgccgg ccagggattc aacctgggcc tgcgcgacgc
     1081 ctgggaactg gcgcgcgcgg cgttggacgc accgcagcgc atcggcgaac ggaacttcat
     1141 cgcccggttc ttgcgcagcc gccgcaccga ccgccgggac accatcctgc tcaccgattt
     1201 gctggtgaac acgttctcca acgatctcgc gcccttgcgc tggctgcgtg ggtgcggact
     1261 tactctgctc gactgccttc cgccggcgaa gcgcgatttc atgggccgga tgatcttcgg
     1321 cgcctgaacg cacgcatggc tcggatcgcg gtttgcacgc ccgccgattg cgttacaatt
     1381 gcgctccttg tcccctgcaa gacgggaatc accaagcccg cgcaatgcag atcggccccc
     1441 accagctcaa gaacaagctc atcgtcgccc ccatggccgg cgtcacggat cgtccgttcc
     1501 gccagctgtg caagcgcctg ggtgccggca tggcggtgtc cgagatgctc tccagccgcc
     1561 ccgagctgcg gcactcggcg aagagcagcc gccgtgccga tcacgcgggg gaagtggagc
     1621 cgatagcggt gcagatcgcc ggcgccgacc cggcgatgat ggccgaggcc gcgcgctata
     1681 acgtggccca gggcgcgcag ctgatcgaca tcaatatggg ctgccccgcc aagaaggtat
     1741 gcaacgtgat ggccggctcc gccctgctcc gggacgagcc gctggtgggg cgcatcctgg
     1801 acgccgtggt caaggcggtg gacgtgccgg tgacgctgaa gatacgcacc ggctgggatc
     1861 gcgagcaccg caacgccgtc agggtggcga gcatcgccga gagcgcgggc gtgcgtgcgc
     1921 tcgccatcca tggccgcact cgcgcctgcg gcttcacggg cagcgccgag tacgacacca
     1981 tcgcggaggt gaagtcgcgc acgcgactgc ccgtcatcgc caacggcgac atcgccaccc
     2041 cggaacaggc ggcgcacgtg ctgcgccata ccggcgcgga ctcgatcatg atcgggcgcg
     2101 ccgcgcaggg ccggccctgg atcttccgcg agatcgccgc gtacctcgat accggccggc
     2161 gcccgcccgg gccgggcttc ggcgagatcc acgcgctgct gcgcgagcat ctgtatgcgc
     2221 tgcacgcctt ctacggcgaa acgatgggcg tgccgatcgc gcgcaagcac gcgggctggt
     2281 acatgcgggc gctgccgggc gcagccgcct ggcgcgcacg cttcaaccgc atcgagacga
     2341 gccagtcgca gctggccgag gtcgaccgct acttcgagga gctggcggcg ctggacacgg
     2401 gagtcggcgc ccaggaggag gagctcgccg catgatcaac gagaacgaga tcgcccgctg
     2461 cgtgcgccgc acgctggacg gctacttccg cgacctggac ggcgagaagg ccgcaggcgt
     2521 ctacaacatg gtgatcagtt gcgtcgagcg gccgctcatc gaggtggtgc tgcaccgcgt
     2581 gggcggcaat cagacgcatg cggcggaact gctgggcatg aaccgcaaca cgctgcgcaa
     2641 gaagatgcgc acctatggaa tcaagtaaag cggggcatcg accaaggggc atcaggtaaa
     2701 aggggcggcg cgcggccgcg tcccgtcttt ccgacccttc tcacttcgag gcatggctgc
     2761 agcacggcag gcgctcatca gtgtttccga caagaccggc gtcgtcgagc ttgcgcgcgg
     2821 gttggtgcgc ctggggttcg gtatcctgtc caccggcggc acggcgcggc tgctcgccga
     2881 ggccggggtg ccggtgaccg aggtcggcga gtacaccggc ttccccgaga tcctcgacgg
     2941 acgggtgaag acgctgcacc cgaaagtgca cgccggcatc ctggcgcgcc gcg
//
LOCUS       DASPGE010000320         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_668115, whole genome shotgun sequence.
ACCESSION   DASPGE010000320 DASPGE010000000
VERSION     DASPGE010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668115"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="VD791_10295"
     CDS             complement(<1..1095)
                     /locus_tag="VD791_10295"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXV08408.1"
                     /translation="MPGPLSGVTVLDLTTVVMGPFATQILAELGADVIKIEPHDGDNM
                     RHVGPMRNRGMGHLYLHLNRGKRCLVLDLKQPEGRDALLRLVPRADALVYNVRPAAMA
                     RLGLAWEALRAVNPRLLYVGCYGYSERGPYAGRAAYDDLIQGASAIPWLMAQDGSAPR
                     YTPINLADRVTGLHVVYAVTAALYRRSVTGQGQAVEVPMFESVAQLVLGDHLAGLSWD
                     PPNGPTGYPRLAHRRPYATKDGYLCVLVYNDKQWRSFLQAVGRDDLLADARFASHANR
                     AQHIGEIYDVLAGLMRGRTSAEWIEVLQRADIPFARMNSAADVVADEHLNATGFFQMQ
                     DHPSEGRMRVMRTPSDWSESPPGALAPAPRL"
     gene            complement(1108..2073)
                     /locus_tag="VD791_10300"
     CDS             complement(1108..2073)
                     /locus_tag="VD791_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXV08409.1"
                     /translation="MVRVAGWALVAGLAGIVALVAQAADRYPSKPIRVIVPFAAGSAT
                     DATARILAQELAKRLDQSVVVENRAGAFGQIAAQAVARSAPDGYTVFITSNTTHSANP
                     HLYRTLPYHPIKDFEPVVKIGLLPFLLVVTPGLPVNSTAELVAYARAHPEALAYGTPN
                     STSLVVTETIKHLAQVSIERVQYKASPEAVLDLTAGRLQVMVADLTIAMPQVKAGKLR
                     VLGVTTAKRTALLPSAPPIADAVPGVDLAGWFGVFVPAGTPKEIVERLSKELLDALSK
                     ADIRKRLADVGVEADPLGPEAFGRFVREQIERWGRVIRAAGIQPE"
     gene            complement(2078..>2206)
                     /locus_tag="VD791_10305"
     CDS             complement(2078..>2206)
                     /locus_tag="VD791_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV08410.1"
                     /translation="GDCCFFPAGVAHVFTVVSDEPVKVLVIYSPPYEENPARAVRS"
BASE COUNT          334 a          754 c          771 g          347 t
ORIGIN      
        1 caggcgcgga gcgggtgcga gcgcaccggg cggggattcg gaccagtcgc tgggggtgcg
       61 catcacgcgc atgcgtcctt cgctcggatg gtcctgcatc tggaagaagc cggtcgcgtt
      121 cagatgctcg tcggccacca cgtcggctgc ggagttcatg cgcgcgaagg gaatgtccgc
      181 gcgctgcagc acctcgatcc attcggcgct ggtgcgcccg cgcatcaatc cggccagcac
      241 gtcgtagatc tcgccgatgt gctgcgcgcg gttggcgtgg ctggcgaagc gcgcatcggc
      301 cagcaggtcg tcgcggccca ccgcctgcag aaagctgcgc cactgcttgt cgttgtacac
      361 cagcacgcac aggtaaccat ccttggtggc gtaggggcgc cggtgcgcca gccgcggata
      421 cccggtcggg ccgttgggcg ggtcccagga cagcccggcg aggtggtcgc cgaggacgag
      481 ctgtgcgacg ctctcgaaca tcggaacctc caccgcctgc ccttgtccgg tgacgctgcg
      541 ccgatatagg gctgcggtca cggcgtacac cacgtgcagg ccggtcacgc gatcggccag
      601 gttgatcggc gtatagcgcg gcgcgctgcc gtcctgcgcc atgagccagg ggatggcgct
      661 cgcaccctgg atcagatcgt cgtaggcagc gcgcccggcg tacggcccac gctcggagta
      721 cccgtagcag ccgacgtaga gcagcctcgg gttcaccgcg cgcagcgctt cccaggcgag
      781 ccccagccgc gccatggcgg ccgggcgcac gttgtacacc agcgcgtcgg cgcgcggcac
      841 cagccgcagc agcgcgtcgc gtccctccgg ctgcttcaag tcgagcacca ggcagcgctt
      901 gccgcggttc agatgcaggt acaggtgtcc catgccgcga ttgcgcatcg gtcccacgtg
      961 gcgcatgttg tcgccgtcgt gcggctcgat cttgatcacg tcggcgccca gctcggcgag
     1021 gatctgggtg gcaaagggac ccatgaccac ggtggtgagg tcgaggacgg tgactccgga
     1081 aagcggtcct ggcatttcgt tcctgactta ttcgggttga atgccggcgg cgcggatgac
     1141 gcggccccag cgttcgatct gttcgcgcac gaagcgcccg aaggcctccg ggccgagcgg
     1201 atcggcctcg acgcccacat ccgccagccg cttcctgatg tcggccttgg agagcgcatc
     1261 gagcagctcc ttcgacaagc gttcgacgat ctccttcggc gttcctgcgg gcacgaacac
     1321 gccgaaccag cccgccaggt ccaccccagg caccgcgtcc gcgatcggcg gagcggacgg
     1381 cagcagcgcc gtgcgcttcg cggtggtcac gccgagcacg cgcagcttgc ccgccttcac
     1441 ctgcggcatg gcgatggtga ggtccgccac catgacctgc aggcgcccgg cggtcagatc
     1501 gaggaccgct tccgggctcg ccttgtactg cacgcgctcg atggaaacct gcgccaggtg
     1561 cttgatggtc tccgtgacca ccagcgacgt gctgttcggg gtgccgtagg ccagcgcctc
     1621 gggatgggcg cgggcatagg cgacgagctc cgccgtgctg ttcaccggca ggccgggcgt
     1681 gaccaccagc aagaacggca gcaggccgat cttcacgacc ggctcgaaat ccttgatcgg
     1741 atggtagggc agcgtacggt acaggtgcgg attcgccgag tgggtggtgt tgctggtgat
     1801 gaataccgtg tagccgtccg gcgcgctgcg cgccaccgcc tgggctgcga tctgtccgaa
     1861 cgcgccggcg cggttctcga ccaccacgct ttgatcgagg cgcttggcaa gctcctgcgc
     1921 caggatgcgc gcagtggcgt ccgtggcgct gccggcggcg aacggaacga tcacgcggat
     1981 cggcttggac gggtagcggt cggcggcctg cgcgacaagc gccacgatgc cggcgaggcc
     2041 ggcgaccagc gcccacccgg cgacccggac catcccctca gctccgcacc gcgcgcgcgg
     2101 gattctcttc gtacggcggc gagtagatca ccaggacttt caccggttcg tcgctcacca
     2161 cggtgaacac gtgcgcaacg ccggccggaa agaagcagca gtcgcc
//
LOCUS       DASPGE010000321         3561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1018116, whole genome shotgun sequence.
ACCESSION   DASPGE010000321 DASPGE010000000
VERSION     DASPGE010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018116"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VD791_10310"
     CDS             <1..895
                     /locus_tag="VD791_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HXV08411.1"
                     /translation="SPALILIDLDPLRDEAVAAVRACHVRWPESPLVAVGSAVDEPAI
                     DAALRAGALAYVPHAYRDTQKLLVLRLALEGVGHRPHFARQEADSPEPGGATETATDS
                     GPRSRKLTRKQVEVLSLAAEGLSNRQIATRLGIAEGTVKLHMSAIYTKLEVDRRGEAI
                     VVARRLQEVRAEQIRHAEGGAQVLDWLLPHVTHRRAARGETIFRKGDLGSGLYYVQRG
                     TVALEEIGVELGPRQILGEIGVFSPEHRRTCTARCLTDVDLFCLDSDQVKSIYYLNPQ
                     FALHVVQLIAQRLLADTDRIR"
     gene            1114..1470
                     /locus_tag="VD791_10315"
     CDS             1114..1470
                     /locus_tag="VD791_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08412.1"
                     /translation="MQAGISIKVFGAITLALLVGSTTSALAADYGRAGGPVGADRIVK
                     VARTTVVSAHSDADFSKWYGNAGGLVGADAIAHVAQTQVTPFGDVDYNQWYGRAGGPI
                     GIAAVKSTEAKIARGK"
     gene            1654..>3561
                     /locus_tag="VD791_10320"
     CDS             1654..>3561
                     /locus_tag="VD791_10320"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXV08413.1"
                     /translation="MLPRFLTQWKTQDTDHRVWPAPFLGALAIGLVAGAFSALPFGQR
                     LEQQVGLWGLFNLRGQAQAPPGVVVIAMRRDTGERVFLPQSRSRDDVCAGLRVDERPP
                     THRALGDVPERWGRCHYVELLRRLALAKPSAVVLDVSFRPRDDLGRREDRALAQAMRA
                     LGNVVLVQRLKIAEDGDGNPVADAPVEISREVAEAALGLAPMPLPILALDRYDEFWTF
                     KEDGYAAPTLPALALQAHALDVYPQFVGSIQRVIPEAALEFPPSAAELTGDGHLHLHA
                     LSVRAVLRQFPERVPQLAQALQGARAGQTRRKLQALLSVYTGPSQRFLNLYGPQGAMT
                     TISMTDILATPHTATSPDPLGLNGKVLFIGFADEKKWEVLEKFPTAMRGPGGGLSGVE
                     LAATAFANLLEGSSLQPTPVWLRASIAFTAGFATALVCYAMTAGLGTLIAVLGLGLYL
                     LAAFLLFKDMQVWAPIFVPAALAAPVALAYAFVSKFLDIKRDRDALDEIMRKFVPSDM
                     VGTLIANRTRLGTVKESTRAACVMTDVEGYTALSTRFAPAQMDALLSRYFAVLFEPVA
                     RNGGFVSDLKGDSILAIWADRGGEASVRVRVCDACLELRDAVDGFNAQHPDTPMPTRF
                     GVNYGEVTLGAV"
BASE COUNT          550 a         1226 c         1238 g          547 t
ORIGIN      
        1 atctcccgcc ttgatcctga tcgaccttga cccgctgcga gacgaggcag tcgccgccgt
       61 gcgcgcgtgc cacgtccgct ggccggaaag ccctttggtc gccgtcggca gcgcggtcga
      121 cgagccggcg atcgacgccg ccctgcgcgc gggggcgctg gcctatgtgc cgcacgccta
      181 cagggacacc cagaagctgc tggtgctgcg cctggccctg gaaggcgtcg gtcaccggcc
      241 gcatttcgca cgccaggaag ccgacagccc cgagccgggc ggcgcgaccg agacagcaac
      301 ggacagcggg ccgcgcagcc gcaagctgac ccgcaagcag gtggaagtgc tctctctcgc
      361 cgccgaaggg ctgtccaacc gccagatcgc cacgcggctc gggatcgccg agggcacggt
      421 gaaactgcac atgtcggcga tctacaccaa gctcgaggtg gaccgccgcg gcgaggcgat
      481 cgtggtggca cggcggctgc aggaagtgcg cgccgagcag atacgccacg ccgaaggcgg
      541 tgcacaggtg ctcgactggc tgctgccgca cgtcacgcac agacgcgccg cgcgcggcga
      601 gaccatcttc cgcaagggcg acctcggcag cgggctgtac tacgtgcagc gcggcaccgt
      661 ggcgctggag gagatcggcg tggagctggg gccacgccag atcctgggcg agatcggtgt
      721 gttttccccg gagcaccggc gcacctgcac cgcgcgctgc ctcaccgacg tcgacctgtt
      781 ctgcctggac tccgatcagg tgaagagcat ctactacctc aacccccagt tcgcgctgca
      841 cgtggtgcag ctcatcgccc agcgcctgct cgcggacacc gaccgcattc gctgagagcg
      901 gtcgccgccc ggcccgagca gcgggcctag aaccttcggc agattttccg ccccggggta
      961 gcgccttagg ctgcgcacac gtatcgaatt cgaccgcaat cgcgtgcagc cgccaaaagg
     1021 cgctgcgccg accggtcagt cgagcgcctg atgctcccgt ggcggcccga ccacccccga
     1081 tacgccgggg gcaacatcgt gaaggagaaa gtcatgcaag caggtatcag catcaaggtg
     1141 ttcggcgcga tcaccctcgc gctgctggtc ggttccacga ccagcgccct ggcggccgat
     1201 tacggccgtg cgggcggccc ggtgggcgcc gaccgcatcg tcaaggtggc tcgcaccacc
     1261 gtggtgagcg cgcacagcga cgccgacttc agcaagtggt acggcaacgc cgggggcctg
     1321 gtgggggcgg acgccatcgc ccacgtcgcc cagacccagg tcaccccctt cggtgacgtg
     1381 gactacaacc agtggtacgg tcgggccggc ggtccgatcg gcatcgctgc ggtgaagtcc
     1441 accgaagcca aaatcgcgcg agggaagtaa gacgagcgcc gtccatccgt gcttggcacg
     1501 gaccgggggt ggggaaagcg tggctctccc cacctccttt ttcctgcagg tatgatccag
     1561 acgcacaaga atgaaatgcc cgcagagttc ttcgccgcga ggctcagcga gtttgaacgg
     1621 cactaggcgg cgccaacgcg gcctggccac gatttgctcc cccgcttcct gacgcagtgg
     1681 aagacgcagg acacggacca ccgggtctgg cctgcgccgt tcctcggggc gctcgcgatc
     1741 ggtctggtcg ccggtgcgtt cagcgccttg ccgttcgggc agcgtctcga gcagcaggtc
     1801 ggattgtggg ggctgttcaa cctgcgcggc caagcgcagg cgccgcccgg cgtggtggtc
     1861 atcgccatgc ggcgcgacac gggcgagcgc gtgttcttgc cgcagtcgcg gtcccgcgac
     1921 gacgtctgcg ccggcttgcg cgtcgacgag cggccgccga cgcatcgcgc gctgggcgat
     1981 gtgcccgagc gttggggacg ttgccactac gtggaactgc tgcggcgcct ggcgctggcc
     2041 aagccgagcg cggtggtcct ggacgtgagc ttccggccgc gcgatgatct cggccgccgc
     2101 gaggaccgtg cgctggcgca ggcgatgcgc gcgctgggca acgtggtgct ggtgcagcgg
     2161 ctcaagatcg ccgaggacgg cgacggcaat ccggtggcgg acgcgccggt ggagatcagc
     2221 cgagaggtcg ccgaggccgc gctcgggctc gctcccatgc cgctgccgat cctggcgctg
     2281 gatcgctacg acgagttctg gacgttcaag gaggacggct atgccgcgcc cacgctgccc
     2341 gccttggcct tgcaggccca tgcgctggac gtctacccgc agttcgtcgg ttcgatccag
     2401 cgcgtcatcc cggaagcggc gctggagttt ccaccaagcg ccgcggaact gaccggagac
     2461 ggtcacttgc acctgcacgc gttgagcgtg cgcgccgtcc tgcgtcagtt tcccgaacgc
     2521 gtgccgcaac tcgctcaggc gctgcagggg gcccgcgccg ggcagacgcg gcgcaagctg
     2581 caggctctgc tgtcggtgta caccggcccg agccagcgct tcctgaacct gtacgggccc
     2641 cagggcgcga tgactacgat cagcatgacc gacatcctcg ccacgccgca caccgcgacg
     2701 tcgccggacc cgctggggct gaacggcaag gtgctgttca tcggcttcgc cgacgagaag
     2761 aaatgggagg tactcgagaa atttcccacc gcgatgcgag gacccggcgg cgggctgagc
     2821 ggcgtggaac tcgccgccac cgccttcgcc aatctgctgg agggatcgag cttgcagcct
     2881 acgccggtat ggctgcgcgc gtcgatcgcc ttcacggccg gcttcgccac ggccctggtg
     2941 tgctacgcga tgacggcggg cctcggcacg ctgatcgccg tgctcggcct ggggctgtac
     3001 ctgctggcag cctttctgct gttcaaggac atgcaggtct gggcgccgat cttcgttccc
     3061 gccgcgctgg cggcgccggt cgcgctcgcc tacgccttcg tcagcaagtt cctcgacatc
     3121 aagcgcgacc gcgatgcgct ggacgagatc atgcgcaagt tcgtgccttc ggacatggtc
     3181 ggcacgctga tcgccaaccg cacgcgcctg ggaacggtga aggaaagcac gcgcgcggcc
     3241 tgcgtgatga ccgacgtcga aggctacacc gcgctcagca cccggttcgc gccggcccag
     3301 atggatgcgc tgctgtcccg gtacttcgcc gtcctgttcg agccggtggc gcgcaacggc
     3361 ggtttcgtct cggacttgaa gggcgacagc atcctggcga tctgggccga ccgcggcgga
     3421 gaagcctcgg tgcgggtgcg cgtgtgtgac gcgtgcctgg agctgcgcga cgctgtggat
     3481 ggcttcaatg cgcagcatcc ggacaccccc atgcccaccc ggttcggcgt caactacggc
     3541 gaagtgacgc tgggcgccgt c
//
LOCUS       DASPGE010000322         2569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_903085, whole genome shotgun sequence.
ACCESSION   DASPGE010000322 DASPGE010000000
VERSION     DASPGE010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903085"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VD791_10325"
     CDS             <1..601
                     /locus_tag="VD791_10325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV08414.1"
                     /translation="ARGDLTDAAAVERVVAEVESALGPVEILVHNAGGDIAAAGGKPD
                     PNDAVTIAEADVRAVLERNLLSTILVCQRVAQGMMARRRGRILTISSVAAFHGRPHGV
                     IYATAKAGVVHYTRCLAAQLRPYDVNVNSLAPGDTRTGRFLGTRPVDPAQLVEDGTLE
                     RIATVDEVARVVEFFAGPLGAFVSGQVLRVDGGTQCWPG"
     gene            1041..2057
                     /locus_tag="VD791_10330"
     CDS             1041..2057
                     /locus_tag="VD791_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HXV08415.1"
                     /translation="MQVTTIGLDIAKNVFQVHGVDEHGKVVLRKPLRRAQVLTFFANL
                     PACRIGLEACAGAHHWARRLAALGHEVRLMPPQYVKAYVKTNKHDAADAEACCEAVQR
                     PGMRFVTIKSEHQQATLLLQRVRDQLIGQRTATINALRGHLAEFGIVAAQRQAGLRQL
                     LAILGDLDDARIPPLAREVLHTLVAHLRQLEERIAELDRRLVARACTDPVCRALCEVP
                     GVGPVIATAFAATVPDAGAFRSGRHLAAWLGLVPRQHATGGKPRQLGLSKRGDGYLRR
                     QLIHGARALLKVSPGRTGRLWSWLNALRARRPFNVAVAALANKLARILWALLSRGEAY
                     RAAA"
BASE COUNT          375 a          939 c          877 g          378 t
ORIGIN      
        1 cgcgcggggc gatctcaccg acgcggcagc ggtcgaacgg gtggtcgccg aggtcgagtc
       61 ggcgctcggg ccggtcgaga tcctggtgca caacgccggc ggcgacatcg cagccgcggg
      121 cggcaagccg gacccgaacg atgcggtgac gatcgccgag gccgacgtgc gcgcggtgct
      181 ggagcgcaac ctgctctcga ccatcctggt ctgccagcgg gtcgcccagg gcatgatggc
      241 gcgccggcgc gggcgcatcc tcacgatcag ctcggtcgcg gcgttccacg gccgcccgca
      301 cggcgtgatc tacgccaccg ccaaggccgg cgtggtccac tacacgcgct gcctggcggc
      361 gcagctgcgc ccctacgacg tcaacgtcaa ctcgctggcg ccaggcgaca cgcgcaccgg
      421 ccgtttcctt ggtacccggc cggtcgatcc ggcgcagctg gtcgaggacg gcacgctcga
      481 gcgcatcgcc accgtggacg aggtcgcccg cgtcgtggag ttcttcgccg gccccttggg
      541 cgcgttcgtc tcgggccagg tgctgcgggt cgacggcggc acccagtgct ggccgggata
      601 gcgcgggccg ccggctgcgg ccgggcgcgg tccggtttta cctctccacc atgtcacccc
      661 gatctggttc accgctccgc cgcatcaccg gctaactgtt gaccgctccg ccatcaccgg
      721 ctgactgttg accgctccgc cacatcaccg gctgagtgtt taccgctgcg ccacatcact
      781 gctgatcggt ttaccgctcc gccacagctg ccatcgccgg ggcctgaccc gcggctgtcc
      841 ggttaacgtt tcgttcagcg tcgcccgcca cgctgctcct gttcgatggt ccatgcgatc
      901 ctgggaggcc atgcgcccgc tgcgccggcc ggcgccggca cggattccca cgatcgcata
      961 atgagatgga cgcctcccgc tggcggcatc ggtgtgccag ggtggtgctg tgaacaacca
     1021 cgaacgcggg agacgtccag atgcaggtta ccacgatcgg cctcgacatt gccaagaacg
     1081 tgttccaggt gcacggcgtc gacgagcacg gcaaggtggt gctgcgcaag ccgctgaggc
     1141 gggcgcaggt gctgacgttc ttcgccaacc tgccggcgtg ccggatcggc ctggaagcgt
     1201 gcgccggagc gcatcactgg gcgcgccggc ttgcggcgct cggccacgag gtgcgcctga
     1261 tgccgccgca gtacgtcaaa gcctacgtca agaccaacaa gcacgatgcc gccgacgccg
     1321 aggcgtgctg cgaggccgtg cagcggccgg gcatgcgctt cgtgacgatc aagagcgagc
     1381 accagcaggc gacgttgctg ctgcagcgcg tgcgcgacca gctgatcggc cagcgcacgg
     1441 cgacgatcaa cgcgctgcgc ggccatctcg ccgagttcgg catcgtggcg gcgcaacgcc
     1501 aagccggtct gcgccagctg ctcgcgatcc tcggcgatct cgacgacgcg cgcatcccgc
     1561 cgctcgcccg cgaggtgctg cacacgctgg tggcccacct gcgccaactc gaggagcgca
     1621 tcgccgaact cgaccggcgt ctggtcgcgc gcgcctgcac cgatcccgtc tgccgcgcgc
     1681 tgtgcgaggt gccgggagtg ggcccggtga tcgccacggc gttcgccgcc accgtgcccg
     1741 atgccggcgc gttccgctcg ggccgccacc tcgccgcctg gctcggcctg gtgccgcgcc
     1801 agcacgccac cggcggcaag ccgcgccagc tcggcctgag caagcgcggc gacggctacc
     1861 tgcgccgcca gctgatccac ggcgcccggg cgctgctcaa ggtcagtccc ggccgcactg
     1921 gccgcttgtg gtcgtggctc aacgccttgc gcgcgcgccg gccgttcaac gtcgcggtcg
     1981 ccgcgctggc caacaagctg gcccgcatcc tctgggcgct gctcagccgc ggcgaggcat
     2041 accgcgccgc ggcctgagac caggacggca atgacctgag cttccaccag ctgcgagggc
     2101 gaacttcgag tgatggcaga tcggtcggac cgaggccggc agagcccgtg ttggtccacg
     2161 agcttcgagc tcgttcgcat gctcgggcgc tggtccgcgg atctcatcga ggccggcggc
     2221 ccaacagccg cgcccgcagg ccgaatggat ggcagcagcc aagaccgcct gccaaagctt
     2281 cgaaaaaagc ccttgcaaag cgggaggcgt ccatggatgg accattgatc ggaccagctg
     2341 accgcaacaa agttaagctc cggttaacgc cctccacgtc tgcgacgcat cgcccggcgc
     2401 cggcattacg cccgtcagca tgcgctccac ccgacagcaa gggctccgcc cggcgatcca
     2461 ggcctgcgtc cggagctctg cccggcacct gaacgtgcgg gtcgcggcgc gtccatgaca
     2521 atcggagctg gcgccttttc gcctggaacg gatagcggca ggtggcgcg
//
LOCUS       DASPGE010000323         6927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1723287, whole genome shotgun sequence.
ACCESSION   DASPGE010000323 DASPGE010000000
VERSION     DASPGE010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723287"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VD791_10335"
     CDS             <1..617
                     /locus_tag="VD791_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08416.1"
                     /translation="LLSSFRKPASDRRTTTGVGFRSLMWILALVGAAGVGGFAYAEYR
                     AQLSRDDLRALRAELQEQMRTLVASAARLEQLQLPGPGAAAAAPESTAIVNLPPCKGA
                     QVGFATQRAPCTEQDALQVAVCAPVPAAARVTAVELFARPEDSQQPWAESRAAAGKEI
                     AAGRFAGSHTERADGEAGKLVCQTFAHWNSKSGRAVRILVRFKA"
     gene            695..1066
                     /locus_tag="VD791_10340"
     CDS             695..1066
                     /locus_tag="VD791_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08417.1"
                     /translation="MTATADAQRLKALKDITTAVYALQAAGFIIGITFIAAVILNYIK
                     LPDVAGTLYESHFRWQIRTFWWGLLWSVIGGLTTWILIGFLILAATAIWVIYRIVKGW
                     LNLSEGKPMEGMPVASSGAAG"
     gene            complement(1082..2257)
                     /locus_tag="VD791_10345"
     CDS             complement(1082..2257)
                     /locus_tag="VD791_10345"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXV08418.1"
                     /translation="MQVEAIDFFYLSMPQILDIGDGSQDALLVRVSGGGCVGYGECEA
                     APLPSIAALVCPMSHSACKPVQASVLGARVESADDIRRIGDAVRANSLDLLQADHTLS
                     GIDMALWDLLGRRLGQPAWKLLGWERSCPKTPYASVLFGDTPAQTLDKARAARARGFA
                     AVKFGWGPFGRAAVQADEDQLAAAREGLGDDGILLVDAGTVWVADVERARAVLPALKR
                     VRATWLEEPFVSGALDEYRALAQDSAPVGLAGGEGSHDFFTAKHMIDHGGVRYVQIDA
                     GRVGGISVARRVADYAQARGVTYVNHTFTSHLALSASLQPYAGLQAHEICEYPAEPKQ
                     LALDITTTHLRPDRDGQVRAPDAPGLGVEVNRAALRPYVVDTEIRVKGRTIYRTPSL"
     gene            complement(2349..3398)
                     /locus_tag="VD791_10350"
     CDS             complement(2349..3398)
                     /locus_tag="VD791_10350"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV08419.1"
                     /translation="MGEQRAEITADSQVARIFEWRRGFNTIYLVHLGVQLGLFRALNE
                     APGSTAAQLAAKLGLHAPYVERWCLTAYGMELLDAQADGGYRLAPFMDVILAAPAHPR
                     YLGGYVQLGTEIAADDFMRCLQAFKTGEVIPFQGRGDAFNHAIAGSTWGLQVVTAKKV
                     LPGLPGLTERLNGGGRVLEVGCGTGNLLVQLAKAFPASQVVGVDIDSDSIAAARSRAG
                     EAGVADRVQVRKGTVAAATQGQQFDAALMVEVLHEIAPALRSGVVADCARALAPGGWM
                     VIVDETYPGTLEEARRPEFKFPLHTGFEEMLWGNVIPTRAEQERLLRDAGLTGRIERS
                     LIGEGFTVLATQKPA"
     gene            complement(3490..4722)
                     /gene="hemW"
                     /locus_tag="VD791_10355"
     CDS             complement(3490..4722)
                     /gene="hemW"
                     /locus_tag="VD791_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HXV08420.1"
                     /translation="MQTTRADPVQSGAIRLTSLPPLALYVHLPWCVRKCPYCDFNSHE
                     LRSAAGTATARSLDSDLERRYLAALLADLDAALPLVEGRIVHTMFFGGGTPSLLSAAG
                     LDTLLTGIRARLRLAADAEITLEANPGTFEADKFAAYRALGVNRLSVGIQSFQPEHLS
                     ALGRIHDVGEAYRAVEIAYASFDNFNLDLMYALPGQTLAQAHADIDRAIALAPPHLSA
                     YHLTIEPNTYFHRFPPRLPDDDLAASMQESIEQRLAAAGYGHYETSAFARAGRECRHN
                     LNYWRFGDYLGIGAGAHGKLSFARRILRQRRWRNPRDYMDKALAGKAVQGAEEVAAED
                     LPFEFMMNALRLTAGFRVSMFQERTGLPFAALLPALRRAESLGLLQFDRHQVAPTLRG
                     QRFLNDLLQLFLPERASG"
     gene            4909..5157
                     /locus_tag="VD791_10360"
     CDS             4909..5157
                     /locus_tag="VD791_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXV08421.1"
                     /translation="MITAAQLRAARALLKMDQRALAEASGLSLPTIQRMEASEGVIRG
                     NVESLMKLIAALDATGVELIGEGASSEKGGRGVRLKAR"
     gene            5249..>6927
                     /gene="hyfB"
                     /locus_tag="VD791_10365"
     CDS             5249..>6927
                     /gene="hyfB"
                     /locus_tag="VD791_10365"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HXV08422.1"
                     /translation="MLGTAAAAVAGARSRATTPLVYSIALGVSAVALSAAGWALLTGT
                     SPAHSVRLPIGLPWLGSHFRLDALSALFLVVIHLGSFTASLFGLGYGRHEPAPGRVLP
                     FFPAFLAGMTLVALADDAFTFLLTWEFMSLASWALVMAHHRHADNARAGYIYLLMASF
                     GTLSLLLAFGLLAGPGGGYAFDAIRGASPSPIVSAAVLMLALIGAGSKAGLVPLHVWL
                     PLAHPAAPSHVSALMSGVMTKVAVYGFVRIAFDLLGPPAWWWGAAVLTLGGITAVLGV
                     LYALMQHDLKRLLAYHTVENIGIIFIGLGLALAFQANGMHSPATLALTAALFHVFNHS
                     VFKSLLFFGSGAVLVATGERDMERLGGLIHRMPLTAIAFLAGSIAISALPPFNGFVSE
                     WLTFQAILVSPGLPQWLLKFLVPAVGAMLALSAALAAGCFVKAFGATFLGRPRTGAAQ
                     RAQETDVFSLTAMFVLVALCLIAGILPGTFIDALGPVTQQLLGAQLPRQTGLEWFAIV
                     PIAQARSSYNGLLLFLFIVAAAWLAATVIRRFASGALRRAPAWDCGFPDASP"
BASE COUNT         1034 a         2428 c         2340 g         1125 t
ORIGIN      
        1 cgctgctcag ctcgttccga aagccggcga gcgatcggcg caccactacg ggcgtcgggt
       61 tccgctcgct gatgtggatc ctcgcgctgg tgggggcggc gggagtcggc ggtttcgcct
      121 acgccgagta ccgcgcgcaa ctgtcgcgcg acgacctgcg cgccctgcgc gccgagctgc
      181 aggagcaaat gcgaacgttg gtcgcctccg cggctcgcct ggagcagttg cagctgccag
      241 gaccaggcgc ggccgcagcc gcgcccgaga gtacggccat cgtcaacctt ccgccttgca
      301 agggtgcgca ggtgggcttc gcaactcagc gggcgccgtg cacggagcag gatgcgctgc
      361 aggtggcggt gtgcgccccc gtacccgccg cggcgcgcgt gaccgcggtc gaactgtttg
      421 cgcggcccga ggactcgcag cagccctggg ccgagtcgcg cgcggcggcc gggaaggaaa
      481 tcgccgcggg acgtttcgcc ggcagccaca ccgaacgcgc ggacggtgag gccggcaagc
      541 tggtgtgtca gactttcgct cattggaaca gcaagagcgg ccgcgccgtg cgcatcctgg
      601 tgcggttcaa ggcatagccg gccgcgatcg ggccgctgtg cgcgcgcaca gggagttgta
      661 ggatgcgcct tccagccgcg cccggagccc gcgaatgacc gccaccgccg acgctcagcg
      721 tctgaaggcc ctgaaggaca tcaccaccgc cgtgtacgcg ctccaggcgg cgggcttcat
      781 catcggcatc accttcatcg ccgccgtcat cctcaactac atcaagctgc cggacgtcgc
      841 cggaacgctg tacgaatcgc acttccgctg gcagatccgc accttctggt gggggttgct
      901 gtggagcgtg atcggcggcc tgacgacgtg gatcctgatc gggttcctca tcctggcggc
      961 gaccgcgatc tgggtgatct accgcatcgt caagggctgg ctgaaccttt cggaaggcaa
     1021 gcccatggag ggcatgccgg tggcgagcag cggcgcggcg ggctgagcgc ccgcaccgcg
     1081 ctcacagcga cggagtgcgg tagatggttc ggcccttgac gcgaatctcc gtgtccacca
     1141 cgtaaggccg caacgccgcg cgattcacct cgacgccgag ccccggcgcg tccggcgcgc
     1201 gcacctgtcc gtcgcgatcc gggcgcagat gcgtggtcgt gatatcgagc gcgagctgct
     1261 tcggctcggc cggatactcg cagatctcgt gcgcctgcag cccggcgtag ggttgaagcg
     1321 acgccgacag cgccagatgc gaagtgaaag tgtggttgac gtacgtcacg ccgcgtgcct
     1381 gggcgtagtc cgcgacccgc cgcgccaccg agataccgcc gacgcgcccc gcatcgatct
     1441 gcacatagcg cacgccgccg tggtcgatca tgtgcttggc cgtgaagaaa tcgtgcgacc
     1501 cctcgccgcc cgccaggccg accggcgcgc tgtcctgcgc gagcgcccga tattcgtcca
     1561 gcgcccccga gacgaacggc tcctccagcc aggtggcgcg cacgcgcttg agcgcgggca
     1621 gcaccgcacg ggcgcgctcg acgtcggcca cccatacggt gccggcgtcg accagcagga
     1681 tgccgtcgtc tcccagcccc tcgcgggcgg cggcgagctg gtcctcgtcg gcctgcacgg
     1741 cggcgcgccc gaacgggccc cagccgaact tgacggccgc gaacccgcgc gctcgcgcgg
     1801 cccgcgcctt gtccagggtc tgcgccggcg tgtcgccgaa cagcacggag gcatagggcg
     1861 tcttcgggca ggaacgctcc cagccgagca gcttccaggc gggttgcccg agccggcggc
     1921 cgagcaggtc ccacaacgcc atgtcgatgc ccgagagcgt gtgatcggcc tgcagaagat
     1981 ccaggctgtt ggcgcgcacc gcgtcgccga tgcgccggat gtcgtcggcc gattccacgc
     2041 gcgcgcccag caccgaggcc tgcaccggct tgcaggcgct gtgcgacatc gggcacacca
     2101 gcgcggcgat cgacggcagc ggcgcggcct cgcactcgcc gtagccgacg cagccgccgc
     2161 cggacacccg caccagcagc gcatcctggc tgccgtcgcc gatgtccagg atctgcggca
     2221 tggacaggta gaagaaatcg atcgcttcga cttgcacgcg gacgctcccg actttcacac
     2281 cgacgccccg acttacaccg gcactccgac tttcacaccg gcaccccgac tttcacagca
     2341 acatcgggtc acgccggctt ctgcgtcgcg agcacggtga agccctcgcc gatcagcgaa
     2401 cgctcgatcc ggcccgtgag cccggcgtcg cgcagcaggc gctcctgctc cgcgcgcgtc
     2461 gggatcacat tgccccacag catctcctcg aacccggtgt gcaggggaaa cttgaactcc
     2521 gggcgacgcg cctcctcgag cgttcccgga taggtctcgt ccacgatcac catccagccg
     2581 ccgggcgcga gcgcacgcgc gcagtcggcc acgacgccgg agcgcagggc gggcgcgatc
     2641 tcgtgcagca cctccaccat cagggcggcg tcgaactgct gcccctgcgt tgccgccgcg
     2701 acggtgccct tgcgcacctg cacgcgatcg gcgacgccgg cttccccggc gcggctgcgc
     2761 gcggccgcga tgctgtcgct gtcgatgtcc acccccacca cctgcgaggc aggaaacgcc
     2821 ttggccagct gcaccagcag gttcccggtt ccgcatccga cctccagcac ccggccgccg
     2881 ccgttcaggc gctcggtcaa ccccggcaat ccgggcagga ccttcttggc ggtgaccacc
     2941 tgcaaccccc aggtcgagcc ggcgatggcg tggttgaacg cgtcgccgcg gccctggaac
     3001 gggattacct cgccggtctt gaacgcctgc aggcagcgca tgaaatcgtc ggcggcgatt
     3061 tccgtcccga gctgcacgta gccgcccaga tagcgcgggt gcgcgggtgc cgccaggatc
     3121 acatccatga acggcgcgag ccgataaccc ccgtccgctt gcgcatcgag cagctccatg
     3181 ccgtaggcgg tgaggcacca gcgctcgacg taaggcgcat gcaggcccag cttcgcagcg
     3241 agctgcgcgg cggtcgatcc cggcgcctcg ttcagcgccc ggaacaagcc gagctgcacg
     3301 cccaggtgga ccagatagat ggtgttgaag ccacgccgcc attcgaagat gcgggcgacc
     3361 tggctgtcgg cggtgatctc ggcgcgctgc tctcccattg cgaaccccct cctggtgcca
     3421 acctccccgt cgcaaacttc cccatcgcga acttccgggc gtcgttgcac gcgcgagtat
     3481 acgccgcgct caaccgctcg ctctctcggg caggaacagc tgcagcaggt cgttcaggaa
     3541 gcgctggccg cgcagcgtcg gcgcgacctg gtggcgatcg aactgcagca gccccagcga
     3601 ttcggcgcgc cgcagcgcgg gcaacagcgc tgcgaacgga aggccggtgc gctcctggaa
     3661 catcgagacc cggaacccgg cggtcaagcg cagcgcgttc atcatgaact cgaagggcag
     3721 gtcctcggcg gcgacctcct ccgccccctg cacggctttc ccggcgaggg ccttgtccat
     3781 gtagtcgcgc ggatttctcc agcgcctctg gcgcaggatg cgccgcgcga aactgagctt
     3841 gccgtgagcg cccgcgccga tacccaggta gtccccgaag cgccagtaat tgaggttgtg
     3901 ccggcattcg cgacccgcgc gcgcgaacgc cgatgtctcg tagtggccgt agccggcggc
     3961 ggccaggcgc tgctcgatcg actcctgcat cgaggcggcg aggtcgtcgt cgggcagccg
     4021 cggcgggaaa cgatggaaat aggtgttggg ctcgatggtc aggtgatagg cggacaggtg
     4081 cggcggcgcg agcgcgatgg cccggtcgat gtcggcgtgc gcctgcgcga gcgtctgacc
     4141 gggcaatgcg tacatcagat ccaggttgaa gttgtcgaag ctggcgtacg cgatctccac
     4201 cgcgcggtac gcctcgccga cgtcgtgaat gcgtccgagc gcggacagat gctctggctg
     4261 gaagctctgg atgccgaccg acagccggtt gacgcccagc gcgcggtagg cggcgaactt
     4321 gtctgcctcg aaagtgccgg ggttcgcttc gagcgtgatc tcggcatccg ccgccagccg
     4381 cagacgcgcc ctgatcccgg tgagcagcgt atcgagcccc gccgcggaca ggaggctcgg
     4441 cgtgccgccg ccgaagaaca tggtgtggac gatgcggccc tcgaccagcg gcaaggccgc
     4501 atcgagatcg gccagcaggg ctgcgaggta gcgccgctcc aggtccgagt ccagcgacct
     4561 ggcggtggct gtgcccgcgg cgctgcgcag ctcgtgcgag ttgaagtcgc aatagggaca
     4621 cttgcgcacg caccagggca ggtgcacgta caaggccaac ggcggaaggc tggtcagccg
     4681 gatcgcgccg gactggaccg gatctgcccg agtagtttgc acggcgcacc tatcgtaccc
     4741 cggcggcagc caatgggttg tcggctcttg cggctccccg gggcaaatgc ggcgagcaat
     4801 gaaggtcaag cccaaagcgg tcgaccggaa ccctcaactt gctaggatca aactgagggc
     4861 tcagtatcaa ctaattagtt catttaccct ataattcatg ttttaaacat gatcactgcc
     4921 gcacaactcc gggcggccag ggcattgctg aagatggacc agcgcgcgct ggcggaggcc
     4981 tccgggctgt cgctgccgac catccagagg atggaggcga gcgaaggcgt gatccgcggc
     5041 aacgtggagt cgctcatgaa gctgatcgcc gcgctcgacg cgacgggcgt ggagctgatc
     5101 ggcgaaggag cgagcagcga gaagggcgga cggggcgtgc gactcaaggc gcgctgaggc
     5161 cccacgcggc tcgggagtcg ccccgagccg agacgacgaa ggcaacgacg acgtggctct
     5221 gacagtcctt ctgtcatgcg ttgcagtatt gctcggaacc gctgcggcgg ccgtcgcagg
     5281 cgcccgcagc cgcgccacca cgccgttggt ctattcgatc gcgctgggcg tcagcgcggt
     5341 cgcgctgtcg gccgctggct gggcgttgtt gaccggcacg agcccggcgc acagcgtgcg
     5401 cctgccgatc ggcctgccgt ggctcggttc gcattttcgg ctcgacgccc tcagcgctct
     5461 gttcctggtc gtcatccatc tcggcagctt caccgcgagc ctgttcggcc tcggctacgg
     5521 ccggcacgag cccgcgcccg gacgcgtgct tccgttcttc ccggcctttc tggcgggcat
     5581 gacgctggtc gcgctcgccg acgacgcgtt cacgtttctg ctgacctggg agttcatgtc
     5641 gctcgcctcg tgggcgctgg tcatggctca ccatcggcac gccgacaacg ccagagccgg
     5701 ctacatctat ctgctgatgg cgagcttcgg caccctgtcg ctgctgctcg ccttcggtct
     5761 cctggccggt cccggcggtg gctacgcgtt cgacgcgatc cgcggcgcgt ctccctcccc
     5821 gatcgtgtcc gccgcggtcc tgatgctggc gctgatcggg gcgggatcca aggccggact
     5881 ggtgccgttg cacgtctggc tgccgctcgc ccatccggcc gcgccgagcc acgtatccgc
     5941 gctgatgagc ggtgtgatga cgaaggtggc ggtgtacggc ttcgtgcgca tcgccttcga
     6001 tctgctgggg ccgccggcct ggtggtgggg tgctgcggtc ctcacgctcg gtggcatcac
     6061 cgccgtcctc ggcgtgctgt atgcgctgat gcagcacgac ctgaagcggc tgctggccta
     6121 tcacacggtg gagaacatcg gcatcatctt catcgggctg ggtctggccc tggcgtttca
     6181 agccaacggc atgcactcgc cggcgaccct ggcgctgacc gccgccctgt ttcacgtatt
     6241 caatcattcc gtcttcaaga gcctgctgtt cttcggttcc ggagccgtgc tcgttgcgac
     6301 cggcgaacgc gatatggagc gtctgggcgg cctgatccat cgcatgccgc tcaccgccat
     6361 cgcttttctg gcgggttcga tcgccatctc ggccctgccg ccgttcaacg ggttcgtgtc
     6421 cgaatggctc acctttcaag cgattctggt gagccctggc ctgccgcagt ggctgctgaa
     6481 gttcctggtc cccgcggtcg gcgcgatgct cgcgctgtcg gccgccctcg ccgcgggttg
     6541 cttcgtcaag gcattcggcg cgaccttcct gggacggccc agaaccggcg ctgcgcagcg
     6601 ggcacaagag accgacgtct tctcgttgac cgcgatgttc gtgctggtgg cgctgtgcct
     6661 gatcgccggg atcctgcccg gaaccttcat cgatgcgctg ggcccagtga cgcagcaact
     6721 gctcggcgca cagttgccgc ggcaaaccgg cctggagtgg ttcgcgatcg tgcccatcgc
     6781 ccaggcccgc agctcctaca acggcctgct gctgttcctc ttcatcgtcg cagcggcgtg
     6841 gctcgcggca accgtcatcc gccgcttcgc ttccggggcg ttgcggcgtg cgccggcgtg
     6901 ggactgtggc tttcccgacg ccagccc
//
LOCUS       DASPGE010000324         6128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_903669, whole genome shotgun sequence.
ACCESSION   DASPGE010000324 DASPGE010000000
VERSION     DASPGE010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903669"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VD791_10370"
     CDS             complement(<1..454)
                     /locus_tag="VD791_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV08423.1"
                     /translation="MKPADKTILAVRGLSANVAGVPILKGIELAIHAGEVHALMGPNG
                     SGKSTLAKVLVGHPAYEVTGGEVLFQGRNLFEMAPEERARAGLFLGFQYPIEVPGVSN
                     GAFLRLAYNTVQGARGQDELDPLEFDDYVQDKLKLLDIDPAFLERSVNE"
     gene            complement(464..1900)
                     /gene="sufB"
                     /locus_tag="VD791_10375"
     CDS             complement(464..1900)
                     /gene="sufB"
                     /locus_tag="VD791_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HXV08424.1"
                     /translation="MSTVLQNLVNQPYRHGFVTEIESETAPKGLNEGTIALISRKKGE
                     PEWLLEYRLKAFRHWQTMAEPTWPNVKYPKIDFQDISYYAAPKPKQKLASMDEVDPEL
                     RRTFEKLGVPLHEQKALAGVAVDVIFDSVSVTTTYKQRLAEVGIVFCSMSEAVRDHPE
                     LVRKYLGSVVPYTDNFYAALNSAVFTDGSFCYIPKGVRCPMELSTYFRINTQESGQFE
                     RTLIVAEESSYVSYLEGCTAPKFDTNQLHAAVVELVALDNAQIKYSTVQNWYAGDENG
                     VGGIYNFVTKRGLCKGINSKISWTQVETGSAITWKYPSCVLLGDNSSGEFYSVALTNH
                     HQQADTGTKMVHIGRNTRSTIVSKGISAGQSNNSYRGLVKIGGKAEGARNYSQCDSML
                     IGSRCGANTFPYIQVQNSSARVEHEATTSKIGEDQLFYFAQRGIGGEEAVSMIINGFC
                     KDVFTQLPMEFAVEATKLLGLKLEGSVG"
     gene            complement(1960..2283)
                     /locus_tag="VD791_10380"
     CDS             complement(1960..2283)
                     /locus_tag="VD791_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HXV08425.1"
                     /translation="MAIQLTERAAQQIQKSLAKRGKGLGLRVGVKKVGCSGWAYTFDY
                     ADQVGANDALFESHDAKLVVASDALPYLDGSTLDYVREGLKESFKFANPNAKSECGCG
                     ESFSV"
     gene            complement(2316..2804)
                     /gene="iscR"
                     /locus_tag="VD791_10385"
     CDS             complement(2316..2804)
                     /gene="iscR"
                     /locus_tag="VD791_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HXV08426.1"
                     /translation="MRLTTKGRFAVTAMIDLALHSGDGPVTLAEISERQRISLSYLEQ
                     LFGKLRRQALVSSVRGPGGGYNLAKPTDQVSVADIIVAVDEPIDATQCGGKENCHDER
                     KCITHDLWASLNERIFDYLRGVTLAQLVLQQQARARDEKVLELKDQRTTRREPSAPAV
                     SA"
     gene            complement(2862..3608)
                     /gene="cysE"
                     /locus_tag="VD791_10390"
     CDS             complement(2862..3608)
                     /gene="cysE"
                     /locus_tag="VD791_10390"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HXV08427.1"
                     /translation="MLDAVREDIRCVFDRDPAARSRWEVITCYPGFHARLCHRLTHRL
                     WGWGFLWLARFLSHIARWLTGIEIHPGARIGRRFFIDHGMGVVIGETAEIGDDVTLYH
                     GVTLGGTTWDKGKRHPTLGDRVVVGAGAKILGPVIVGVGAKVGSNAVVVKDVPPGCTA
                     VGVPGRIVEPGREEERQRAAEKLGFSAYGISSDMNDPVVQAIHGLLDHVVNIDARLEE
                     ILGRLEQAGIQTGEMKKDGFDPTYLNKIVD"
     gene            complement(3675..4433)
                     /locus_tag="VD791_10395"
     CDS             complement(3675..4433)
                     /locus_tag="VD791_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXV08428.1"
                     /translation="MTTPTPHAAADSPLARVRVVLVETSHPGNIGAAARAMKTMGIEA
                     LALVRPRHFPHDEAQAMASRATDILAAARVCGTLDEALAGTVFAVACTARSRDLSHPA
                     LTPREAAARMVEEARRGPVALVMGPEKYGLKGQDVNRCSVISTVPANPEYSSLNLAAA
                     VQLFAYEVRLAAERIETLPQENHPPAPHEDVEQFIAHLEQTMIEIGFLDPASPKRLMQ
                     RMRRLFARARLEREEVNILRGILAAAQAAARRKT"
     gene            4535..5359
                     /locus_tag="VD791_10400"
     CDS             4535..5359
                     /locus_tag="VD791_10400"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXV08429.1"
                     /translation="MALNHPMVNIAVKAARRAGRIINQAAGNLDVLTVRHKSLNDLVS
                     EVDRGAEAAIIDTLKTAYPEHAILAEESGAAGRSEYVWVIDPLDGTTNYLHGFPQYCV
                     SIALLHKGVISHGVIFDPTRNDLYTASRGRGAFVNDRRMRVSRRSKLIDTLIGTGFPF
                     RMFEHMDAYVAMMRDLMTRTAGIRRPGAAALDLAAVAAGRLDGFWEIGLSPWDMAAGV
                     LMIQEAGGLVGDLQGEDRYMERGDIVAGNPKIFAQLLQVIRPHLTPALREGPRVQA"
     gene            5438..>6128
                     /locus_tag="VD791_10405"
     CDS             5438..>6128
                     /locus_tag="VD791_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HXV08430.1"
                     /translation="MERKTQLTIWYWILALMALLWAQSIWTEMRGVEAIPYSQFQQLL
                     KDGQVRDIAITSNAIRGTLKSPLPDGSTRFVTTRVDPGLAGQLDQYGVKYEQRVESTF
                     LRDILSWIIPAVVFFALWMFLMRRFAEKQGLGGGFLSIGKSKAKVYIETDTKVTFADV
                     AGVDEAKDELKEVVAFLKAPKEYGRLGARSPKGVLLVGPPGTGKTLLARAVAGEAGVP
                     FFSITGSEFVEM"
BASE COUNT          946 a         2046 c         2042 g         1094 t
ORIGIN      
        1 cttcgttgac gctgcgctcg aggaacgccg gatcgatatc cagcagcttg agcttgtcct
       61 gcacgtagtc gtcgaactcc agggggtcca gctcgtcctg gccgcgcgcg ccctgtaccg
      121 tgttgtaagc caggcgcagg aaggcgccgt tgctcacgcc cggcacctcg atcgggtact
      181 ggaagccgag gaacaggccg gcccgcgcgc gctcctcggg cgccatctcg aacaggttgc
      241 gtccctggaa cagcacctcc ccgccggtca cttcgtaggc cgggtgtccc accaggacct
      301 tggccagcgt gctcttgccc gagccgttcg ggcccatcag ggcgtgcacc tcgccggcgt
      361 ggatggcgag ctcgatgccc ttgaggatgg gcacgccggc cacgttggcg ctcagcccac
      421 gcaccgccaa gatcgtcttg tcagccggct tcatgttcat gggttatccg acgctgccct
      481 cgagcttcaa gcccagcaac ttggtcgcct cgacggcgaa ctccatcggc agctgggtga
      541 acacgtcctt gcagaagccg ttgatgatca tcgacaccgc ctcctcgccg ccgatgccgc
      601 gctgcgcgaa gtagaacagc tggtcctcgc cgatcttgga ggtggtggcc tcgtgctcca
      661 cccgcgcgct gctgttctgc acctggatgt aggggaaggt gttggcgccg cagcggctgc
      721 cgatcagcat cgagtcgcac tgcgaatagt tgcgcgcgcc ttccgccttg ccgccgatct
      781 tcaccagccc gcggtagctg ttgttggact gccccgcgga gatgcccttg ctgacgatgg
      841 tgctgcgcgt gttcctgccg atatggacca tcttggtgcc ggtgtcggcc tgctggtggt
      901 ggttggtcag cgccaccgag tagaactcgc cgctggagtt gtcgcccagc agcacacagg
      961 acggatactt ccaggtgatg gccgaaccgg tctcgacctg ggtccaggag atcttggagt
     1021 tgatcccctt gcacaggccg cgcttggtga cgaagttgta gatgccgccc acgccgttct
     1081 cgtcgccggc gtaccagttc tgcaccgtgg agtacttgat ctgcgcgttg tccagcgcca
     1141 ccagctccac caccgccgcg tgcagctgat tggtgtcgaa cttgggcgcc gtgcagcctt
     1201 ccaggtagga cacgtagctg ctttcttcgg ccacgatcag ggtacgctcg aactgccccg
     1261 actcctgcgt gttgatgcgg aagtaggtgg acagctccat cgggcagcgc acgcctttgg
     1321 ggatgtagca gaagctgccg tcggtgaaca ccgccgagtt gagcgcggcg tagaaattat
     1381 cggtgtaggg caccaccgag ccgaggtact tgcgcaccag ttcggggtgg tcgcgcaccg
     1441 cctccgacat cgaacagaag acgatgccga cctcggccag gcgctgcttg taggtggtgg
     1501 tgaccgacac gctgtcgaag atcacgtcca cggccacgcc ggcgagcgcc ttctgctcgt
     1561 gcagcggcac gcccagcttc tcgaaggtgc ggcgcagctc gggatcgacc tcgtccatcg
     1621 acgccagctt ctgcttcggt ttgggcgcgg cgtagtagct gatgtcctgg aagtcgatct
     1681 tcgggtactt gacgttcggc caggtcggct cggccatggt ctgccaatgg cggaacgcct
     1741 tcagccggta ctcgagcagc cactccggct cgcccttctt gcgcgagatc agcgcgatgg
     1801 tgccctcgtt cagccccttg ggcgcggttt cggactcgat ctcggtgacg aagccgtgcc
     1861 ggtagggttg attgaccaga ttctggagaa ctgtgctcat ggattcgatg cttctgcccg
     1921 ctcacgcctg gcggcgggct tcgagagcgc gccgcgcctc tagacgctga agctctcgcc
     1981 gcacccgcac tcgctcttgg cgttcgggtt ggcgaacttg aaggactcct tcaggccttc
     2041 gcgcacgtag tcgagcgtgg agccgtccag gtagggcagc gcatcgctcg ccaccaccag
     2101 cttggcgtcg tgcgactcga acagcgcgtc gttggcgccg acctggtcgg cgtagtcgaa
     2161 agtgtaggcc cagccggagc agcccacttt cttcacgccc acgcgcagcc ccagaccttt
     2221 gccgcgcttg gccagcgact tctggatctg ctgggcggca cgctcggtga gttgaatcgc
     2281 catctgtcgt tcctgaagta tcggggtgtg caccttcacg ccgagacggc cggcgcgctc
     2341 ggctcgcgcc gtgtcgtgcg ctgatccttg agctccaaca ccttctcgtc ccgggcgcgc
     2401 gcctgctgct gcagcacgag ctgcgccagc gtgacgccgc gcaggtagtc gaagatgcgc
     2461 tcgttcaggc tcgcccacag gtcgtgggtg atgcacttgc gctcgtcgtg gcagttctcc
     2521 ttgccgccgc actgggtggc gtcgatcggc tcgtccacgg cgacgatgat gtcggccacc
     2581 gacacctggt cggtcggctt ggccaggttg tagcccccgc ccgggccgcg cacgctggat
     2641 accagcgcct ggcgccgcag cttgccgaac aactgctcca ggtaggagag cgagatcctc
     2701 tgtcgctccg agatctcggc cagcgtcacg ggcccgtcgc cgctgtgcag cgccaggtcg
     2761 atcatcgcgg tcaccgcgaa acgtcccttt gtagtcaatc gcatggcagt tcctgaagga
     2821 tatccgacta ggacgctcaa ctataccaaa gcctgattgt ttcagtcaac tattttgttc
     2881 agataagtgg ggtcgaagcc gtcctttttc atctcgccgg tctggatgcc ggcctgttcc
     2941 aggcgtccca ggatctcctc gaggcgggcg tcgatgttga ccacgtggtc gagcaggccg
     3001 tggatcgcct gcactacggg gtcgttcatg tccgagctga tgccgtaggc ggagaacccc
     3061 agcttttcgg cggcgcgctg gcgctcctcc tcgcggcccg gctcgacgat gcggcccggc
     3121 acgcccacgg cggtgcagcc ggggggcacg tccttcacca ccaccgcgtt ggagccgacc
     3181 ttcgcgccta cgcccacgat caccggcccc aggatcttcg cccccgctcc caccaccacg
     3241 cggtcgccca gcgtgggatg gcgcttgccc ttgtcccagg tggtgccgcc cagcgtgacg
     3301 ccgtggtaca gggtcacgtc atcgccgatc tcggcggtct cgccgatcac cacgcccatg
     3361 ccatggtcga tgaagaagcg gcgcccgatg cgcgcgccgg ggtggatctc gataccggtc
     3421 agccagcgcg cgatgtgcga caggaaacgc gccagccaca ggaagcccca gccccacagg
     3481 cggtgcgtga gccggtgaca cagccgcgcg tgaaaacccg gataacaggt aatgacttcc
     3541 cagcggctac gcgcggcggg gtcgcggtcg aaaacgcagc ggatgtcttc gcggacggcg
     3601 tccagcatcg gtgtgccacc tgttgtagtc ggtcaagcgc ctggttccca ccagtgtact
     3661 taaggaagct ctccttaggt tttccggcgg gccgcagcct gcgccgcggc caggatgccg
     3721 cgcaggatgt tgacttcctc gcgctcgagc cgcgcgcgcg cgaacagcct gcgcatgcgc
     3781 tgcatcaggc gcttgggcga ggccgggtcg aggaagccga tctcgatcat ggtctgctcg
     3841 aggtgcgcga tgaactgctc cacatcctcg tgcggcgccg gcggatggtt ctcctgcggc
     3901 agcgtctcga tgcgctcggc ggcgagccgc acttcgtagg cgaacagctg caccgccgcg
     3961 gccaagttca gcgaggaata ctcgggattg gcgggtaccg tggagatgac gctgcagcgg
     4021 ttgacgtcct gtcccttgag gccgtacttc tccgggccca tgaccagcgc caccggtccg
     4081 cgccgcgctt cctcgaccat gcgcgcggcg gcctcgcgcg gcgtgagcgc cgggtgcgac
     4141 aggtcgcggc tgcgcgccgt gcatgcgacc gcgaacacgg tgccggcgag cgcttcgtcc
     4201 agcgtgccgc acacgcgcgc ggcggcgagg atgtcggtgg cgcgcgaggc catcgcctgc
     4261 gcttcgtcgt gcgggaagtg cctgggcctg accagcgcca gggcttcgat gcccatggtc
     4321 ttcatggcgc gcgcggcggc gccgatgttc cccggatggc tggtctccac cagcacgacg
     4381 cgcacgcgcg ccagcggcga gtcggcggcc gcgtgtggcg tgggggtagt cacgcaagta
     4441 gaatgcgcgc tttgccggcc ggacgccacc cgtcgcttgc caagacgcag ggacgttccg
     4501 acagcgcggg ctctttgcac atcactggaa cgagatggcg ctcaaccacc ccatggtcaa
     4561 catcgcggtg aaagccgcgc ggcgcgccgg gcgcatcatc aatcaggcgg cgggcaacct
     4621 ggatgtgctc acggtccggc acaagagcct gaacgacctg gtgtccgagg tcgaccgcgg
     4681 cgccgaggcc gcgatcatcg acacgctgaa gacggcctat ccggagcacg cgattctagc
     4741 agaggagagc ggcgcggccg gccgctccga gtatgtctgg gtcatcgacc cgctggacgg
     4801 caccaccaac tatctgcacg gctttccgca gtactgcgtg tcgatcgcgc tgctgcacaa
     4861 gggcgtcatc agccacggcg tgatcttcga tccgacccgc aacgatctgt acaccgcctc
     4921 gcgcgggcgc ggggctttcg tcaacgaccg gcgcatgcgc gtgtcacgcc gcagcaagct
     4981 gatcgacacg ctcatcggca ccggcttccc gttccgcatg ttcgagcaca tggacgctta
     5041 cgtggcgatg atgcgggacc ttatgaccag gaccgccggc atccgccgcc cgggcgccgc
     5101 cgcgctcgat ctggcggcgg tggcggcggg ccgcctggac ggcttctggg agatcggcct
     5161 gtcgccctgg gacatggccg cgggggtgct gatgatccag gaggccggcg ggctggtggg
     5221 cgacctgcag ggcgaggacc gctacatgga gcgcggcgac atcgtggccg gcaacccgaa
     5281 gatcttcgcg cagctgctgc aggtgattcg gccgcacttg acgccggcgc tgagggaggg
     5341 gccgagggtc caggcctagc cctttgccaa ccgacggcgc attgaacctt cccttgccgc
     5401 cctcatctag attgtcattg cccggacaag agctcccatg gaacgaaaga cacagctcac
     5461 catctggtac tggatcctgg cgctgatggc cctgctctgg gcgcagagca tctggaccga
     5521 gatgcgcggg gtcgaggcga tcccgtacag ccagttccag cagctgctga aggacgggca
     5581 ggtgcgggac atcgccatca ccagcaacgc catccgcggc acgctgaaga gccccctgcc
     5641 cgacggcagc acacgcttcg tcaccacccg cgtggacccg ggcctcgcgg gccagctcga
     5701 ccagtacggc gtcaagtacg agcagcgggt cgagagcacg ttcctgcgcg acatcctgtc
     5761 ctggatcatc ccggcggtgg tgttcttcgc cctgtggatg ttcctgatgc ggcgcttcgc
     5821 cgagaagcag ggcctgggcg ggggcttcct gtcgatcggc aagagcaagg ccaaggtgta
     5881 catcgagacc gacaccaagg tcaccttcgc cgatgtcgcg ggcgtggacg aggccaagga
     5941 cgagctgaag gaggtcgtgg cctttctcaa ggcgcccaag gagtacgggc ggctgggcgc
     6001 gcgcagtccc aagggcgtgc tgctggtggg gccgcccggc accggcaaga cgctgctcgc
     6061 gcgcgcggtg gccggcgagg ccggcgtacc gttcttctcg atcaccggat ccgagttcgt
     6121 ggagatgt
//
LOCUS       DASPGE010000325         5881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1608157, whole genome shotgun sequence.
ACCESSION   DASPGE010000325 DASPGE010000000
VERSION     DASPGE010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5881
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608157"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VD791_10410"
     CDS             complement(<1..727)
                     /locus_tag="VD791_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HXV08431.1"
                     /translation="MTYLAKPKMHHPALEKNRLGYTRRDYEGSVSTLCAGCGHDSISA
                     AIIQACFELELPPHRIAKLSGIGCSSKTPTYFLGSSHGFNSVHGRMPSVLTGANLANR
                     DLIYLGVSGDGDSASIGLGQFAHCLRRGVNMVYIVENNGVYGLTKGQFSATADKGSKS
                     KKGAVNPDSPIDLVGLALQLGATFVARSFSGDRAQLVPLVKAAMVHRGAAFLDVISPC
                     VAFNNHPGSTKSFDHVREHNEAVN"
     gene            complement(769..2616)
                     /locus_tag="VD791_10415"
     CDS             complement(769..2616)
                     /locus_tag="VD791_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HXV08432.1"
                     /translation="MPQQAQRTERINNFVIKFANVNGSGSASANALFAKSILRMGVPV
                     TPRNIFPSNIQGLPTWYEVRVSEAGYLGRRGGVDLMVAMNPQTWDKDVAEIEPGGYLF
                     YDSTKPLPKSKFRDDVHVIGMPLTEICNREYVDARERQLFKNIIYVGALSALLGVDPK
                     EVERLIAEQYRGKEKLLEPNIKALHMGRDYAANYLPSIALHVRRADAVGERVFLDGNS
                     AAALGALYGGATVAAWYPITPSSSLAEAFARHCRKYRVDPDTGRNRYAIVQAEDELAA
                     VGIAIGAGWNGARAFTATSGPGISLMQEFIGLAYFAEIPAVIFDVQRGGPSTGMPTRT
                     QQSDVLACAYASHGDTKHVLLFPEDPKECFEMAALAFDLADRLQTPVFVLSDLDIAMN
                     ERLCDPLAWDDARRYDRGKVMSFQDLEAGRDFGRYLDVDGDGIPYRTYPGTHPTRGAY
                     FTRGTTKDRYARYSEAGPDYVDNMQRLLRKFETAKELVPAPRRRQAAQPARCGVIYFG
                     STGPAMCEALDLLEAQGLHLDALRVRAFPFRDEVSEFIAEHDQVFVVEQNRDAQLRML
                     LGNECVVDPARLIPILHFDGNPITARFIAGAIAQQMKAMNVAPLRRVAQ"
     gene            complement(2571..4406)
                     /locus_tag="VD791_10420"
     CDS             complement(2571..4406)
                     /locus_tag="VD791_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXV08433.1"
                     /translation="MNPTDTAAPDYFHKVVDCQWACPAHTPVPEYIRLVAQGRYSDAY
                     MINWRSNVFPGILGRTCDRPCEPACRRGRVEEGNEGKAEPVAICRLKRVAADYKDDIR
                     GRLPRPAKQKNGKRIALVGAGPASLTVARDLAPLGYSVTLFDGDTWAGGMIRTQIPRF
                     RLPEHVIDEEVGYIIDLGVDFRPGQRVDSLAALLAEGYDAVFIGSGAPRGRDLDIPGR
                     REAAANIHIGIDWLASVSFGHISRIGKRVIVLGGGNTAMDCCRSSRRLGGEDVKVVVR
                     SGFAEMKASPWEKEDAMHEGIPILNYLVPKVFTHDNGRLTGVRFEKVSAVHDADGRRQ
                     LLPTGEPEVHLECDDVLLAIGQENAFPWIERDIGIEFDRSGMPRVDALTMQSTRPGVL
                     FGGDAAFGPKNIIWAVAHGHDAAITIDRLCHGEDPRQRPPPMVNLASQKMGIHEWSYD
                     NAISGDQRYRVPLKETRITLKDIRMEVELGFDPKLAFAEAQRCLNCDVQTVFTPKLCI
                     ECDACMDICPVDCINFTDNGEEPELRGRLRAPATDATQALYVSDALKTGRVMVKDEDV
                     CLHCGLCAERCPTGAWDMQKFYLDMTHAGQACRSKHKEQSASTTS"
     gene            complement(4542..5180)
                     /locus_tag="VD791_10425"
     CDS             complement(4542..5180)
                     /locus_tag="VD791_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08434.1"
                     /translation="MRRIALLCAGLLLSPAIGAQVDGTKTQPAAGGQPTAVPAAGANS
                     LSERSAPLVRELEARYKQSMQAARRGDVEAYWSMRTAASRTRPPQLDTARLRLLADLL
                     PPLEALHFVRVDTTGKTARALYRWRKEDVAQYSVITYRMEGGEWKIDDVSVRRGGSTA
                     GAPVVSAPPQPADPPASARPGAPASGSQAQELLKAWESGESATGRSLSAPRL"
     gene            complement(5362..>5881)
                     /locus_tag="VD791_10430"
     CDS             complement(5362..>5881)
                     /locus_tag="VD791_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV08435.1"
                     /translation="GIQSFAPSALVELYGVPVTLATSSITAYMVASAGGMITGGFLAA
                     RTSHHERIIAGAFAVAGALCMLVASGTVSPASTVVLLALIGFGSGIAGPARDLLVRAA
                     APRGATGRVYGVVYSGLDVGMSIAPLMFGAMMDGGHPGWVFVAIGLFQALALFTAIGL
                     GGSTQRARAQPA"
BASE COUNT          875 a         1970 c         2081 g          955 t
ORIGIN      
        1 ggttcaccgc ctcgttgtgc tcgcgcacgt ggtcgaagct cttggtcgat ccgggatggt
       61 tgttgaaggc gacgcacggg ctgatgacgt cgaggaacgc cgcgccccgg tgcaccatgg
      121 ccgccttgac cagcggcacg agctgggccc ggtcgccgga aaagctgcgc gccacgaagg
      181 tggcgcccag ttgcagcgcc agcccgacca ggtcgatcgg cgagtccggg ttgaccgcgc
      241 ccttcttgct cttggatccc ttgtccgcgg tggcggaaaa ctggcccttg gtcaagccgt
      301 agacgccgtt gttttcgacg atgtacacca tgttcacgcc gcggcgcagg caatgggcga
      361 actgccccag gccgatggac gccgagtcgc cgtcgccgga cacgcccagg tagatcagat
      421 cgcggttggc gagattggca ccggtcagca ccgagggcat gcgcccgtgc acgctgttga
      481 agccgtgcga gctgcccagg aagtaggtgg gcgtcttcga cgaacagccg atgcccgaca
      541 gcttggcgat gcggtgcggc ggcagctcca gctcgaagca ggcctggatg atggcggcgc
      601 tgatcgagtc gtgcccgcag cccgcgcaca gcgtggacac ggacccctcg taatcgcggc
      661 gcgtgtaccc gagccgattc ttctccaggg ccgggtgatg catcttcggt ttggccagat
      721 acgtcattcg tcacgctcct cggtcacgct tcccggtcat gccctggctc actgcgccac
      781 ccgccgcaac ggcgccacgt tcatcgcctt catctgctgc gcgatcgccc cggcgatgaa
      841 gcgcgcggtg atcgggttac cgtcgaagtg caggatcggg atcaggcgtg cggggtccac
      901 gacgcattcg ttgccgagca gcatgcgcag ctgcgcgtcg cggttctgct ccaccacgaa
      961 cacctgatcg tgctcggcga tgaactcgct gacctcgtcg cggaatggaa aggcacgcac
     1021 ccgcagcgca tcgaggtgca gcccttgtgc ctccagcagg tcgagcgctt cgcacatcgc
     1081 cggaccggtg gaaccgaagt agatgacgcc gcagcgcgcg ggctgcgctg cctggcgccg
     1141 gcgcggcgcc ggcaccagct ccttggcggt ttcgaacttg cgcagcagcc gctgcatgtt
     1201 gtccacgtag tccgggccgg cctcgctgta gcgcgcgtag cggtccttcg tggtgccgcg
     1261 cgtgaagtag gcgccgcgcg tggggtgcgt gcccgggtag gtgcggtagg ggatgccgtc
     1321 tccgtccaca tccaggtagc gcccgaagtc gcgcccggcc tccaggtcct ggaagctcat
     1381 caccttgccg cggtcgtagc ggcgcgcgtc gtcccaggcg agtgggtcgc acaggcgctc
     1441 gttcatggcg atgtccagat cggacagcac gaacaccgga gtctgcaacc ggtcggccag
     1501 atcgaaggcc agcgcggcca tctcgaagca ctccttggga tcctcgggga acagcagcac
     1561 gtgcttggtg tcgccgtgcg aggcgtaggc gcaggcgagc acgtcgctct gctgcgtgcg
     1621 cgtgggcatg ccggtggagg ggccgccgcg ctgcacgtcg aagatcaccg cggggatctc
     1681 ggcgaagtag gcgagcccga tgaactcctg catcagcgaa atgccgggtc cagaggtggc
     1741 ggtgaacgca cgcgcgccgt tccagccggc gccgatcgcg atgccgacgg cggcgagctc
     1801 gtcctcggcc tgcacgatgg cgtaacggtt cctgccggtg tcggggtcca cccggtactt
     1861 gcggcagtgg cgcgcgaagg cttccgccag cgacgacgag ggcgtgatcg ggtaccacgc
     1921 ggccaccgtg gcgccgccgt acagcgcgcc cagcgcggcg gcgctgttgc cgtccaggaa
     1981 cacgcgctcg cccacggcgt cggcgcgccg cacgtgcaag gcgatggagg gcaggtagtt
     2041 ggcggcgtag tcgcgcccca tgtgcaacgc cttgatgttg ggttcgagca acttctcctt
     2101 gccccggtac tgctcggcga tgagccgctc gacctccttc gggtccacgc cgagcagcgc
     2161 ggacagcgcg ccgacgtaga tgatgttctt gaacagctgc cgctcgcgcg cgtcgacgta
     2221 ctcgcggttg cagatctcgg tgagcggcat gccgatcacg tgcacgtcgt cgcggaactt
     2281 cgacttgggc agcggcttgg tgctgtcgta gaacaggtag ccgccgggct cgatctcggc
     2341 cacgtccttg tcccaggtct gcgggttcat cgccaccatc aggtcgacgc cgccgcgccg
     2401 ccccaggtag ccggcctcgc tcacgcgcac ctcgtaccag gtgggcaggc cctggatgtt
     2461 ggaaggaaag atgttgcgcg gggtgaccgg cacacccatg cgcaggatgg acttggcgaa
     2521 cagcgcgttc gcggaggccg agccggagcc gttgacgttg gcgaacttga ttacgaagtt
     2581 gttgatgcgc tctgttcttt gtgcttgctg cggcatgcct gccccgcgtg tgtcatgtcc
     2641 aggtagaact tctgcatatc ccaggcgccg gtcgggcagc gctcggcgca caggccgcag
     2701 tgcaggcaca cgtcctcgtc cttgaccatg acgcgcccgg tcttgagcgc gtcggagacg
     2761 tacagcgcct gggtggcgtc ggtcgccggc gcgcgcagcc tgccgcgcag ctccggctcc
     2821 tcgccgttat cggtgaagtt gatgcaatcg acggggcaga tgtccatgca ggcatcgcat
     2881 tcgatgcaca gcttgggcgt gaacaccgtc tgcacgtcgc aattcagaca gcgctgcgcc
     2941 tcggcgaagg cgagcttggg atcgaagccc agctccacct ccatgcggat gtccttgagc
     3001 gtgatgcggg tttccttgag cggtacgcgg tagcgctggt cgccggagat ggcgttgtcg
     3061 tagctccact cgtggatgcc catcttctgg gacgcgaggt tgaccatcgg cggcgggcgc
     3121 tggcgcgggt cctcgccgtg gcacagccgg tcgatggtga tggccgcatc gtggccgtgg
     3181 gccaccgccc agatgatgtt cttcggtccg aacgcggcgt ccccgccgaa cagcacgccc
     3241 ggccgggtgg attgcatggt gagcgcatcc accctgggca tgcccgagcg gtcgaactcg
     3301 atgccgatgt cgcgctcgat ccagggaaac gcgttttcct ggccgatggc gagcagcacg
     3361 tcgtcgcact ccaggtgcac ttcgggctcg ccggtgggca gcaactggcg gcggccgtcc
     3421 gcgtcgtgga cggcggagac tttctcgaac cgcacgccgg tgaggcggcc gttgtcgtgg
     3481 gtgaagacct tgggcacgag gtaattgagg atcgggatgc cctcgtgcat ggcgtcctcc
     3541 ttctcccagg gcgaggcctt catctctgcg aagccggagc gcaccaccac cttcacgtcc
     3601 tcgccgccca ggcggcgcga ggaccggcag caatccatcg ccgtattgcc cccgccgagc
     3661 acgatcacgc gtttgccgat gcgcgagatg tgaccgaagg acacgctggc gagccagtcg
     3721 atgccgatgt ggatgttggc cgccgcttcc ctgcggccgg ggatgtccag gtcgcggccg
     3781 cgcggcgcgc cgctgccgat gaacaccgcg tcgtagccct ccgccagcag cgccgcgaga
     3841 ctgtccacgc gctggccagg gcggaaatcc acgccgagat cgatgatgta gccgacctct
     3901 tcgtcgatga cgtgctcggg cagtcggaag cgcggaatct gcgtgcggat catgccgccc
     3961 gcccacgtat ccccgtcgaa cagcgtcacg gagtagccca gcggcgccag gtcgcgggcc
     4021 accgtcagcg aggcagggcc cgcgcccacc agggcgatgc gcttgccgtt cttctgcttc
     4081 gcgggcctgg gcaaccgccc gcggatgtcg tccttgtagt cggccgccac gcgcttcagc
     4141 cggcagatgg cgaccggctc ggccttgccc tcgttgcctt cctccacccg gccgcggcgg
     4201 caggccggct cgcaggggcg gtcgcaggtg cgtccgagaa tgccggggaa gacgttggac
     4261 ctccagttga tcatgtaggc gtcgctgtag cgaccctgcg cgaccagccg gatgtattcg
     4321 gggacggggg tgtgggcggg acacgcccat tggcaatcga ctaccttgtg gaagtagtcg
     4381 ggtgccgcgg tatcagtcgg attcaacact cctccagctt ccccggcccg gattcgcgct
     4441 tcttgttgca ctgcgggagg gggcgccccg cccgtgccgc gccgagtggc cggaatggta
     4501 cgccgcgcgc gcagggctga aaagggatga taacgaagcg gttatagccg cggcgcgctg
     4561 agcgaccggc cggtcgccga ctcaccggac tcccaggcct tgagcaattc ctgtgcttgc
     4621 gagcccgagg cgggtgcgcc gggccgtgcc gatgcgggcg ggtcggccgg ttgcggaggg
     4681 gcggacacga ccggcgctcc ggcggtgctg ccgccccggc gcaccgagac gtcgtcgatc
     4741 ttccactcgc cgccctccat gcgataggtg attaccgagt actgcgccac gtcttccttg
     4801 cgccagcggt atagcgcgcg cgcggtcttg cccgtggtgt ccacccgcac gaagtgcagc
     4861 gcctccaggg gcggcaggag gtcggcgagc agacgcagcc gggcggtgtc gagctgcggc
     4921 gggcgggtgc gcgaggctgc cgtgcgcatg ctccagtagg cctccacatc gccgcggcgg
     4981 gcggcctgca tcgattgctt gtagcgcgct tcgagctcgc gcaccagcgg tgcgctgcgt
     5041 tccgacagac tgttggcgcc cgcggccggc accgcggtcg gctggccgcc ggcggccggc
     5101 tgcgtcttgg tgccatcgac ctgcgcgccg attgccggcg aaagcagcaa tcctgcgcac
     5161 agcaaagcga ttcgcctcat tgcgaccttc ctccgcctgc gggcaatcgg ccggcgcggc
     5221 gccgagcgcc cgataagagg gatttcgaca cccgggcctg ggcctttagc gggacccaag
     5281 gcgtgagcgg cagagacggc gcgggtcggc aggaagacgc tggggcggcg tgcagatgct
     5341 ggactcggcc gggtgcggcg ctcacgccgg ttgcgcacgc gcgcgttgcg tgctgccgcc
     5401 cagcccgatg gcggtgaaca acgccagggc ctggaacagg ccgatggcca cgaacaccca
     5461 gcccggatgg ccgccgtcca tcatcgcccc gaacatgagc ggcgcgatcg acatgcccac
     5521 gtccaggccg gagtacacga cgccgtacac ccggccggtc gcaccgcgcg gggccgcggc
     5581 acgcaccagc agatcgcgcg cggggccggc gatgccggag ccgaagccga tcagggcgag
     5641 cagcacgacg gtgctcgccg gcgacacggt gccgctcgcc accagcatgc acagcgcacc
     5701 ggccacggcg aacgcgcccg cgatgatgcg ttcgtgatgg ctggtgcgcg cggccagaaa
     5761 accgccggtg atcatgccgc cggcgctggc gaccatgtac gcggtgatcg agctggtggc
     5821 gagcgtaacc ggtacgccat acagctccac cagcgcggag ggggcgaagc tctggattcc
     5881 g
//
LOCUS       DASPGE010000326        18107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1372028, whole genome shotgun sequence.
ACCESSION   DASPGE010000326 DASPGE010000000
VERSION     DASPGE010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18107
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372028"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VD791_10435"
     CDS             <1..1186
                     /locus_tag="VD791_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXV08436.1"
                     /translation="ANRAALWLTYIAVGAGLATLLVWIALGTPLSFAVERMVAVMVIS
                     CPHALGLAVPLVVAVSTTLAARHGLLIRDRAAFERARKLDAAVFDKTGTLTEGRFGVT
                     DVVPFARTDARELTAVAAALESQSEHPIAKGIVEHAQTLGVEVRRATDFRNVTGQGAV
                     ARLDGQPIAVVSPGYVQQRGLMVDDPRLERLQAEAKTLVFVLRADRPLGAIALADIVR
                     PESREAVRRLKRMGIACLMLTGDNRSVAAAVARELELDEYFAEVLPADKAGKIREVKQ
                     RGLTVAMVGDGVNDAPALVESDLGVAVGAGTNVAIESADVVLVRSNPSDVVAILSLSR
                     STYRKMVQNLWWATGYNAVAIPLAAGVTAGLGFFLSPAVGAVFMAASTVIVAINAQLL
                     RG"
     gene            complement(1183..2349)
                     /locus_tag="VD791_10440"
     CDS             complement(1183..2349)
                     /locus_tag="VD791_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08437.1"
                     /translation="MWKPILTALLAGCVTAARAQSCIPPSADSPPLYRVLAKLPRAGL
                     RTALDEHAFRSRWARHDEAVASGFRYGDAVYGSVLRKDGRFDPQATADWKRQHSRSPA
                     RAELRRALGTLADPLTLDRAAVQWLLASCLRTETWSALRPIDACRFELRAGLQPGAAP
                     APPARPTRFSVRGGRCAAWPEKPLSKSGDSVECVRSGNGDFGVELLSDTAEAIRVRLP
                     ALPAGKPIPEPVKQEQLSEPISEVISLHRTRDFKAVYLGRGCPTCRVYAADVRPTPDG
                     AVILGIVTALSSGAGWHRCPAGLRCGVYEFSPPDAPGQSGCVGLAACRVWRLAETDGE
                     ASDVVQLTYQVAQVTCVNCPPGMDYDTARKTWEERTKAEACPVVPDAPAQVFGR"
     gene            2496..3128
                     /locus_tag="VD791_10445"
     CDS             2496..3128
                     /locus_tag="VD791_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08438.1"
                     /translation="MKTVLTVLALGWLTAASCAWAQDDPHAGHHPPAAGQATDGAAGQ
                     TEGHDPAADGGQHEHGKAGGKLQENMQRMQALMVRMRETTDPTQRRALLGEHLQVMRD
                     QVKLMLAQAGGHAHGGQAGAGAAEAAQEAHGAQPSQSSEAGKKKKCAMMEGGKCKMME
                     DKKGGMMADEMMGSGMMMMHQKTEQRVHMLEQLLEQMVEREAVEHELESR"
     gene            3198..3548
                     /locus_tag="VD791_10450"
     CDS             3198..3548
                     /locus_tag="VD791_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08439.1"
                     /translation="MSRFVELALALLVLGLSTSITPAHAAVCPTPGGDDGLALLAQQP
                     AHAAQLLADCSEKCASGGQRELMLLVDDIPSPRHLPLVSGADEVASWHAEIFEVLSVR
                     REDAEALEYCQLML"
     gene            complement(3616..4170)
                     /locus_tag="VD791_10455"
     CDS             complement(3616..4170)
                     /locus_tag="VD791_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08440.1"
                     /translation="MTHPRPSFVLTGRRIEQGLDQLDRLYRLARELCPDEFVAEETRE
                     LKALYPHVSLGLIDSLRAELAHQLDLPPEIFQRDRFSIHGCGPVGLHDDFFRFPYVYF
                     AIVVAHAGRLGLVDAQRRAVAHRPGDIILLDPRAKHALVPAGVTAEEHPYESSHGPVR
                     DEALQFLFLDFDVRRPDLRARFRR"
     gene            complement(4167..4595)
                     /gene="msrB"
                     /locus_tag="VD791_10460"
     CDS             complement(4167..4595)
                     /gene="msrB"
                     /locus_tag="VD791_10460"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HXV08441.1"
                     /translation="MEENPRFRPSISAMDKIDRPDDEWRKLLTPEQYKVLRKEGTERA
                     GTSPLNQEKRKGSYLCAGCGAPLFASDTKFDSGTGWPSFYRALPGALETKPDNSLFMA
                     RTEYHCARCGGHQGHVFDDGPQPTGQRYCNNGLALRFEPD"
     gene            4986..5342
                     /locus_tag="VD791_10465"
     CDS             4986..5342
                     /locus_tag="VD791_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08442.1"
                     /translation="MALLFAQLATAAYACPELAPPDGTSSAAPASDCEAAHAVSRSDE
                     EDGLLCKASCEQGAQVLKASSGFDAAKTAVVLYLAPSISSRDLVSSSVALAVSGFDRR
                     PPGWPPAYLLHHVLRN"
     gene            5451..6731
                     /locus_tag="VD791_10470"
     CDS             5451..6731
                     /locus_tag="VD791_10470"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXV08443.1"
                     /translation="MPNRLCSARRKPSLPWIAALAGLLLPADVSPADQQAGLSFIEAL
                     DAAVARAPTLAARRATAEGAATARVSAGRLPDPRLAVGVDNLPVTGDEAFSLTDDFMT
                     MTKIGWMQEVPNRDKRKARADVASALADREHALLTAEAQAVKRETALAWLKRYFAEKR
                     LTLFAPLESENRVLQETVNARIAAGRALPVEATQARQEAVLLADRRDELTRERAQAQA
                     ALKRWVGERADAPLSGEPPSLTVDPAHLLHNLERHVELAAFDPMARMAAAEAREAQAG
                     KKGDWGWEVSYAQRGPAFSEMVSFQVSFELPLFASKRRDPQIFAKRKEVERIAAERQE
                     TLRKHAEEIEAELAEQAQLARQLARLRDSAVPLAEERVRLLMASYRAGRTDLGLVLLA
                     RQERNETRLKAVELEGALAALRARLAYLIAEHAS"
     gene            6728..8248
                     /locus_tag="VD791_10475"
     CDS             6728..8248
                     /locus_tag="VD791_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV08444.1"
                     /translation="MSAIRTLGVVAIAGAVVLAGAAGFGVANWRAEHSMRNSDRPPSD
                     RGSAPSAAKEGERQVLYWYDPMYPQHKFDQPGKSPFMDMQLIPKYADEVSASGVKIDP
                     TLAQNLGMRLATVERQTMSTGVSAVATVGFNERDVAIVQARTGGFVERVYARAPGDVV
                     RAGAALADVLVSEWAGAQAEFLAVKATGDAELTAAARERLRLLGMTEAQIAQVERSGQ
                     LSAVMTITFPIAGVIQELGIRAGMSVTPGMTLARINGLRTVWLEAAVPEVHAAMLVAG
                     RRVEALFSAYPGEVFEGKIAAVLPEADRDTRTLRVRMEFANRGQRLKAGMFAQVTIAG
                     ESKEALMVPADAIIRTGKRALAFVSEQPGQFTPVEVEVGQEMDGKLTVLSGLEEGQQV
                     AVSGQFLIDSEASLSGLTQRMGEGSTAAGDTNTQAAAGPIHEGIGTVEELSGTAVTLK
                     HGPIASLQWGPMTMPFSLPSPDVAKDLKAGDSVRFRFRQVEGNFVIESIEKTGGGR"
     gene            8245..11430
                     /locus_tag="VD791_10480"
     CDS             8245..11430
                     /locus_tag="VD791_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV08445.1"
                     /translation="MIEKLIHWSVHNRFLVLLATVFVSAWGVWALRTTPIDALPDLSD
                     VQVIIRTTYPGQAPQIVENQVTYPLATTMLSVPGAKTVRGYSFFGDSFVYVLFEDGTD
                     LYWARSRVLEYLNQVQGRLPATAKTALGPDATGVGWIYQYALVDRTGRHDLSQLRALQ
                     DWFLRFELKSLPNVAEVASVGGMVKQYQVVLDPFKLAAFGISHTRVREALDEANRETG
                     GSVLELAEAEYMVRASGYLRSLDDFRAVPLAVVGGVPVKLGDVAIVQLGPEMRRGIAE
                     LDGEGEVAGGVVLLRSGKNAQETIAAVKTKLAELQRSLPKGVEIVPTYDRSHLIQRAV
                     ENLSHKLMEEFAVVALVCVLFLWHLRSALVAIISLPIGVLIAFVVMKHQGINANIMSL
                     GGIAIAIGAMVDAAVVMIENAHKKVEAWQHAHPGQRLHGEEHWKVMTEAAAEVGPALF
                     FSLLIITLSFIPVFTLQAQEGRLFGPLAFTKTYAMAAAAGLAVTLVPVLMGYWIRGRI
                     PEETKNPLNRVLIAAYRPLLEAVLRFPKATLAIAMLLFASMLWPLARLGGEFLPPLDE
                     GDLLYMPSALPGLSAAKASQLLQQTDRMIKTVPEVERVFGKAGRAETATDPAPLEMFE
                     TTIQLKPREEWRPGMTPERLADELDRVVRVPGLANIWIPPIRNRIDMLATGIKSPIGV
                     KVSGTDLAAIDRVAAQVEQAAKGVPGVSSALAERLAGGRYIDVQIDRQAAARYGLNIA
                     DVQSVIASAIGGENIGETVEGLARFPINLRYPRELRDSPEKLRQLPIVTPIGQQITLG
                     TVARVETTDGPPMLKSENARLSGWVYIDVRGRDLASVVADLRRAVVREVTPAPGIGIA
                     YSGQFEFLERANARLKVVVPATLVIIFVLLYLTFKRFDEALLIMVALPFALTGGVWFL
                     YLLGYNLSIATGVGFIALAGVAAEFGVVMLLYLKHALQVRTGSDAAAPIEVLDDAIRE
                     GAVLRVRPKAMTVAVILAGLLPIVWGGGTGSEVMSRIAAPMLGGMVTAPLLSMFVIPA
                     AYKLMRRQRLRAGADATEPLEARALRFAARR"
     gene            11513..11938
                     /locus_tag="VD791_10485"
     CDS             11513..11938
                     /locus_tag="VD791_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08446.1"
                     /translation="MQALNWRHTHHYVQGESVEVGDVPRHVVGIIRGSGLGFFDDGAV
                     ATHSIVIVHDFTDGTGPHSGYVTYAFEDGATLSLSYRGTSTTPAGQNSTLIEGKFTFL
                     RGSGRFSGVAGSGDYRGKRLIPAAGGGEVYLDFVGTRSR"
     gene            complement(11994..12374)
                     /locus_tag="VD791_10490"
     CDS             complement(11994..12374)
                     /locus_tag="VD791_10490"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXV08447.1"
                     /translation="MIERHVIPGQPAPVSHYCHVVRADRHVWVSGSVGIKPDGSIPET
                     TVEQFRVALASVDACLRQVGAKPRHVVKVTVFMTDISERPLINPQRIDYFGDNRPAST
                     LVEVSKLVDPRLKVEIEAVAYIDD"
     gene            complement(12396..13358)
                     /locus_tag="VD791_10495"
     CDS             complement(12396..13358)
                     /locus_tag="VD791_10495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXV08448.1"
                     /translation="MNAVQVRPPALLLLCTMILAPASVGAEVAPLVLGTATAGGGFAV
                     YGQAVADTLSETDPGLQIQLQATGGSTENIPLLEAGRLDLALVEGTTAYEAMTGIGRA
                     PADLRLVAVMYPSPGMFVVRGDSRYRTIRDLRGERVVFGAATSGLVVLARYVLDGLGL
                     DLRRDFDAVLLERVADGPPMVLRGEAAALWGAGVGWPGFAAVARGPQAARFIGPEPDD
                     LARIRAKHPFLQTMTVAAQSYPGQHQAITTVGTWSLILARPGLPDEAAYRFARALHLG
                     QAALGQRLAQARDTTPANTLAAAHRPELLHPGVARYFRDIGLIR"
     gene            13452..14135
                     /locus_tag="VD791_10500"
     CDS             13452..14135
                     /locus_tag="VD791_10500"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HXV08449.1"
                     /translation="MVKEPDPIEWRVPVGTETVVAVYEPAAASGGAALFVCAHGAGGH
                     RNDSGMQRLAEQLRGRGIDMVRFNFLYREKGARAPDPMPRLMDCFAAVASHARRETQA
                     DLLIIGGRSMGGRAASMLAAQGFACDALLLLAYPLHPAGKPEQLRDAHLARIKMPVLC
                     LNGTRDNLCRRDLMDTIVAGLPDSWTMHWLEAADHGFHVPRTSGRSDSDVLAEIGEVI
                     ERWVLHGAL"
     gene            complement(14285..14422)
                     /locus_tag="VD791_10505"
     CDS             complement(14285..14422)
                     /locus_tag="VD791_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08450.1"
                     /translation="MSRIVIADDDPGIGRILRDRLRELGHDAEHVLDGVEALRLADSA
                     A"
     gene            14406..15533
                     /locus_tag="VD791_10510"
     CDS             14406..15533
                     /locus_tag="VD791_10510"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV08451.1"
                     /translation="MTILLIMRAMRAGAYDFIAKPFDAATIQLVVKRALETRGLKTQV
                     HSLRKELGRKHLWVRGADPGMARIAETVERIAPSTATVLLLGETGTGKEVIARAIHLQ
                     SPRREQPFVAVNCAVLKGELLESELFGHEKGAFTGADRARPGRVEAARGGTLFLDEIA
                     ELPPNLQAKLLRVLQEKEYERLGSDRTQRADIRLVAATNRDLPAAIQAGAFREDLYYR
                     LKVISIKIPPLRERPSDILALAEWLLGQHAAESGRTPPRLSEEVEQALLRYSWPGNVR
                     ELSNVMERCVLLACDTVGLCDLPEELMGGAAAPGAVTKVEDLFALEYNAAVTAARRLI
                     VAKALEQTGGHQTRAAERLGVTQPYLSRLVKQLGIRRDDQH"
     gene            complement(15496..16035)
                     /locus_tag="VD791_10515"
     CDS             complement(15496..16035)
                     /locus_tag="VD791_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXV08452.1"
                     /translation="MIAPVFVDTNVFLYARDAGETAKQPRAAAWLEHLWREQIGRTSV
                     QVLSEYYVNVTRKLSPGLPAEEAWDDVQALLTWNPQPVDDALLRRGREIELRYRLGWW
                     DSLVVGAAQLQGCALLLTEDLQDGGVFGSVAVRSPFTLRLEQAASVYAVAPGAARRHP
                     PRGRPKAVSAGRRAGSPTA"
     gene            complement(16013..16255)
                     /locus_tag="VD791_10520"
     CDS             complement(16013..16255)
                     /locus_tag="VD791_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HXV08453.1"
                     /translation="MKNVTITLDEKTATWARVYAAKHNTSVSRMVGEMLQQRMRESHE
                     YDEAMRRFLAKKPVRLKRAGGRYATREETHDRSRVR"
     gene            complement(16359..17573)
                     /locus_tag="VD791_10525"
     CDS             complement(16359..17573)
                     /locus_tag="VD791_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HXV08454.1"
                     /translation="MGGAAATARTDRAAFYAELQRLKQHFEAMQPELEVTLRDPALDV
                     EGYVVVWNTGISAGGPLPGCAKGGTRITPNASLDEVKMLARTMALKNAAAGLPLGGAK
                     SGLKLDPSAPGFEERYRRFVRLCAPLLYENGGPFGGFGFDIGARPEHALWACDELKST
                     RCFTGKPVHMGGTDYDREGIAGLGVAVAGGTMLEVKGESPAQASFAVQGMGAMGAAVL
                     RYFSETGARLAALGDPRYGGTWTFDAPPSADLHGALARQDSETAKPLLANEGTCVSRD
                     PQEVLYLETDILFPCAVQNVITEHNAPRVQARYVCEGANAPVSEAARTSLHARGVALV
                     PDFIANPGGVIAAFVELTSQSADKAEEAKSLTREKIAANVRELFAIAERHRAEPQHAG
                     LYMALAKISQTS"
     gene            complement(17605..>18107)
                     /locus_tag="VD791_10530"
     CDS             complement(17605..>18107)
                     /locus_tag="VD791_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXV08455.1"
                     /translation="RLVLAAAGNGVRAVVMCPTMIYGRGRGAHQDSVQVPRLIALAKK
                     YGVGRHVGPGENIWSNVHIDDLVSLYLLALQKAPAGAFYYAENGENSYREVAAAISRM
                     LGFGGRTQPLSMQEAVAELGEAAANYSFGSNSRVRAVRARRELGWKPNGPALLDEIER
                     GAYAER"
BASE COUNT         2918 a         6053 c         6330 g         2806 t
ORIGIN      
        1 agcgaatcgt gccgccctgt ggctgaccta tatcgccgtc ggggcagggt tggccacgct
       61 gctggtgtgg attgcgctcg gcacaccgct gtccttcgcc gtggagcgca tggtcgcggt
      121 gatggtcatc tcctgcccgc acgcgctggg cctggcggtg ccgctggtag tcgcggtcag
      181 caccacactg gccgcccggc acgggctgct gatccgcgat cgcgccgcct tcgagcgcgc
      241 ccgcaagctg gacgcggcgg tgttcgacaa aaccggcacg ctgaccgagg ggcgctttgg
      301 cgtgaccgac gtggttccct tcgcgcggac cgacgcgcgc gagctgaccg cggtggccgc
      361 cgcgctcgag tcgcagtcgg agcatccgat cgcgaaaggc atcgtggagc acgcgcagac
      421 gttgggcgtc gaggttcggc gcgccaccga cttccgcaac gtcaccggcc agggcgcagt
      481 cgcccggctc gacgggcaac ccatcgccgt ggtcagcccc ggatacgtcc agcagcgcgg
      541 gctcatggtc gatgatccgc gcctggagcg cttgcaggcg gaagcgaaga ccctggtgtt
      601 cgtcctgcgc gcagaccggc cgctcggcgc catcgcgctc gccgacatcg tccggccgga
      661 gtcgcgcgaa gcggtccggc gcctgaagcg catggggatc gcctgtctca tgctgaccgg
      721 agacaaccgc agcgtcgccg ccgcggtggc gcgcgagctg gagctcgacg aatacttcgc
      781 ggaggtgctg cccgcggaca aggccggcaa gatccgggag gtgaagcagc gcggtctgac
      841 ggtggcgatg gtgggcgacg gcgtcaacga cgcgccggcg ctggtggaat ccgatctcgg
      901 cgtcgccgtg ggggcgggaa ccaacgtcgc gatcgagtcc gcggacgtgg tgctggtgcg
      961 cagcaacccg tccgacgtgg tcgcgatcct gtcgttgtcg cgcagcacct accgcaagat
     1021 ggtgcagaac ctgtggtggg ccaccggcta caacgccgtg gccatcccgc tggccgcggg
     1081 cgtgaccgca gggctcgggt tctttctgtc gccagcggtg ggtgcggtgt tcatggcggc
     1141 gagcacggtg atcgtcgcga tcaacgcgca gctgctgcgc ggctagcgcc cgaacacctg
     1201 cgccggcgcg tccggcacca ccggacacgc ctccgctttg gtgcgctcct cccaggtctt
     1261 gcgcgccgtg tcgtaatcca ttcccggcgg gcagttgacg caggttacct gcgcgacctg
     1321 ataggtgagc tgaaccacgt cggaggcctc gccgtcggtt tcggccaggc gccacacgcg
     1381 gcaggccgcg agcccgacgc agccgctctg ccctggagcg tcgggcggag agaactcgta
     1441 gacgccgcag cgcagccccg cagggcagcg atgccagccc gcgccgctcg acagggcggt
     1501 gacgatgccg aggatgacgg cgccgtccgg cgtcgggcgc acgtcggcgg catacacgcg
     1561 gcaggtgggg cagcccctcc cgagatagac cgccttgaag tcgcgcgtgc gatgcagcga
     1621 gatcacctcc gagatcggct ccgacaattg ttcttgcttc accggctcgg ggatgggttt
     1681 gccggccggc agcgccggca gccggacccg aatcgcctcg gccgtgtcgg acagcagttc
     1741 gacgccgaaa tcaccgttgc cgctgcgcac gcattccacg ctgtcgcctg atttcgacag
     1801 tggcttctcg ggccaggccg cgcagcggcc gccgcgcacc gaaaagcgcg tcgggcgcgc
     1861 cggtggcgcg ggggcggccc ccggctgcag gcccgcgcgc agctcgaagc ggcaggcgtc
     1921 gatgggcctc agcgcgctcc aggtctcggt gcgcaggcag ctcgccagca accactgcac
     1981 cgcggcacgg tccagggtca gcgggtcggc gagcgtgccg agcgcgcgac gcagctcggc
     2041 gcgggcgggc gagcgggaat gctgccgctt ccagtccgcc gtggcctgcg gatcgaagcg
     2101 cccgtccttg cgcaggacgc tgccgtacac cgcatcgcca taacggaagc cggacgcgac
     2161 cgcctcgtca tgccgtgccc accggctgcg gaacgcgtgc tcgtcgagcg ccgtgcgcaa
     2221 gccggcgcgg ggcagcttcg ccaggacccg gtacagcggg ggactgtcgg ccgagggcgg
     2281 aatgcaggac tgcgccctcg ccgcggtcac gcaaccggcg agcagcgcag tgaggatcgg
     2341 tttccacatg agcgggcgga tggctcgagc caaacccgca ttatccgcga agggggattt
     2401 catgatctag ctcaaccggc gcgggaacca gggcgctaga ctgcacgcat tacttcgcag
     2461 gcgcgatggg actttcgagg agcgaggcaa gagccatgaa gacagtactt acggtgttgg
     2521 cgctcggttg gctgacggca gcgtcctgcg cgtgggcgca ggacgatccc cacgccgggc
     2581 accatcctcc ggccgccggc caggcgaccg acggcgcggc agggcaaacc gaaggacacg
     2641 acccggccgc ggacggcggc cagcacgagc acgggaaggc cggcggcaag ctgcaggaga
     2701 acatgcagcg gatgcaggcg ctgatggtgc gcatgcggga gacgacggat ccgacgcaac
     2761 gtcgtgcgct gctcggcgag cacctgcagg tgatgcggga tcaggtcaag ctgatgctgg
     2821 cccaggcggg cggtcacgcg cacggcggcc aggccggtgc aggcgcagcg gaagccgcgc
     2881 aggaagcgca cggcgcgcag ccgagccaga gcagcgaagc cggcaagaaa aagaaatgcg
     2941 ccatgatgga aggcggcaag tgcaagatga tggaagacaa gaagggcggc atgatggccg
     3001 acgaaatgat gggaagcgga atgatgatga tgcaccagaa gacggagcag cgcgtgcaca
     3061 tgctggagca gctcctggag cagatggtcg agcgcgaggc ggtggagcac gagctggaat
     3121 cgcgctgacg gcgtgggcaa gcgattgcgc gctgtagtat gctgtgcgcc tacccgctca
     3181 tgccgtggga ggctcgcatg tcacgattcg tcgaactcgc cctcgcgttg ctggtgctcg
     3241 gtctctccac ctccatcaca ccggcgcacg ccgccgtatg cccgacgcct ggcggcgacg
     3301 acgggcttgc gctgctcgcg cagcagcccg cgcacgcggc gcagctgctg gccgactgca
     3361 gcgagaagtg cgcatccggc gggcagcgcg agttgatgtt gctggtagat gacattccct
     3421 ccccgcgtca ccttccgctg gtgtccgggg cggacgaagt cgcgtcctgg cacgccgaga
     3481 tcttcgaggt gctgagcgtc cggcgggagg acgcggaggc gctggagtac tgccagctga
     3541 tgctgtaagc gcccgtccgc gttgcgtttc cccgaatccg caccgcgctt gccgcggtgc
     3601 gggggcgttc tcaacctacc ttcgaaagcg cgcccgcaga tccgggcgcc gcacgtcgaa
     3661 atccaggaat aggaactgca gcgcctcgtc gcgcaccggg ccgtggctgc tttcgtaggg
     3721 atgctcctcg gcggtgacgc ccgcgggcac cagcgcgtgc ttggcgcgcg ggtcgagcag
     3781 gatgatgtcg cccggccgat gcgcgacggc gcggcgctgc gcatcgacca ggccgaggcg
     3841 gccggcgtgc gccaccacga tggcgaagta gacgtagggg aagcggaaga agtcgtcgtg
     3901 cagccccacc ggcccgcaac catggatgga gaagcgatcg cgctggaaga tctccggcgg
     3961 caggtcgagc tggtgcgcga gctccgcgcg cagactgtcg atcaacccca gggacacgtg
     4021 gggatagagc gccttcagct cgcgggtctc ctcggcgacg aactcgtcgg gacacagctc
     4081 gcgcgccagg cgatagagcc ggtcgagctg gtcgaggccc tgctcgatgc gccggccggt
     4141 gaggacgaag ctcggacggg gatgggtcaa tccggctcga accgcagtgc caggccgttg
     4201 ttgcagtagc gctggccggt gggctgcggg ccgtcgtcga acacgtggcc ctggtgcccg
     4261 ccgcagcgcg cgcagtggta ttcggtgcgg gccatgaaca gcgagttgtc gggcttggtt
     4321 tccagggccc ccggcagcgc ccggtaaaag ctgggccagc cggtgccgct gtcgaacttg
     4381 gtgtccgaag caaacagcgg cgcgccgcag ccggcgcaca ggtagctgcc cttgcgcttc
     4441 tcctggttga gcgggctggt gcccgcgcgc tcggtgcctt ccttgcgcag caccttgtac
     4501 tgctcgggcg tgagcagctt gcgccactcg tcgtcggggc ggtcgatctt gtccatggcc
     4561 gaaatcgaag gtcgaaacct cggattttcc tccaatggcg cggcggagtg aaggcgagcc
     4621 ttggattctc cctaggcagg ctgcgcattc gggcctgcgg caattcatga ttttcgaggc
     4681 tgcgccgcgg gcacgtcgcg cgcttttaac ctggatgaat gatgcgcgga gcggaaagtc
     4741 ctactctttc tagtttcgcg cgtcccggtg gagcggcgga accgccaccg tcaacgggaa
     4801 gccgaaatga aagcgtgatc cgggcgtgaa ggttgcatgt catgattgat gctgctggcc
     4861 cgggggacga gatcgcgcgc ccgccttgcg atccacccgg acacgcccaa cgtataatgc
     4921 agcaaagcgg agtgggattc gtgcataagg tcagatcgcg gcgttggctt tccggctgga
     4981 tgatcttggc gttgctgttc gcacagctcg ccacggccgc gtacgcctgc cccgagctcg
     5041 ccccgccaga cgggacgagc tcggctgcac ctgcttccga ctgcgaggca gcacacgccg
     5101 tctcgcgaag cgacgaggaa gacggcctgc tgtgcaaggc gagctgcgaa cagggggcgc
     5161 aggtcctcaa agcctcaagc ggcttcgacg ccgccaagac ggcggtcgtg ctctacctcg
     5221 ccccctccat ttcgagccgc gatctcgttt cgtcctccgt cgcgctcgcc gtgtcgggct
     5281 ttgaccggcg cccgcccggc tggccgccgg cctatctgct ccatcacgtt ctccgcaact
     5341 agcctgtaga gggcccggcg ccgcagtccc ggcgccagac cgcatcgggc ctgcgcaccc
     5401 gggtgcgcgc ccgtgcgtca attcctcttt cacgcagcgg aggacgcgca atgccgaacc
     5461 gcctttgttc cgctcggcgc aagccgagtc tgccctggat cgcagcgctg gctgggctgc
     5521 tgctgcccgc cgatgtcagc ccggccgacc agcaagccgg cctctccttc atcgaggcgc
     5581 tcgacgccgc cgtcgcgcgg gcgcccacgc tcgccgcccg ccgggcaacc gcggaaggcg
     5641 ccgcgaccgc ccgggtcagc gccggacgac tgcccgatcc caggctcgct gtcggagtcg
     5701 acaacctgcc ggtcaccgga gatgaggcct tcagcctgac cgacgacttc atgaccatga
     5761 ccaagatcgg ctggatgcag gaagtaccca accgggacaa gcgcaaggcc cgcgccgatg
     5821 tcgcaagcgc gctggcggac cgcgagcacg cgctgctgac cgccgaagcg caggcggtga
     5881 agcgggaaac cgcgctcgcg tggctcaagc gctacttcgc ggagaagcgg ctcacgctgt
     5941 tcgcgcccct ggagtccgag aatcgggtgt tgcaggaaac cgtcaacgcc cggatcgccg
     6001 ccggccgggc tttgccggtg gaagcgaccc aggcgcggca ggaggcggtg ctgctcgccg
     6061 accggcgcga cgagctgacc cgcgagcggg cacaggcgca ggcagcgctc aagcgctggg
     6121 tgggcgagcg ggcggacgcg ccgctttccg gtgagccgcc gagtctgacg gtcgatcccg
     6181 cgcacctgct ccataacctc gaacgccacg tcgagctggc ggcgttcgat cccatggcgc
     6241 gcatggctgc cgccgaggcg cgcgaggcgc aagccggcaa gaaaggcgac tggggctggg
     6301 aggtgagcta cgcgcaacgc ggcccggcct tcagcgaaat ggtgtcgttc caggtgagct
     6361 tcgagctgcc gctgttcgcc tcgaagcggc gcgacccgca gatcttcgcc aagcgcaagg
     6421 aggtcgagcg catcgcggcc gagcgccagg agacgctgcg caagcacgcg gaagagatcg
     6481 aagcggagct cgccgagcag gcccagctcg cgcgccagct cgcgcggctg cgcgacagcg
     6541 ccgtgcccct ggcggaagag cgcgtgcggc tcctgatggc cagctatcgc gccggacgca
     6601 cggacttggg cctggtgctg ctcgcgcgcc aggagcgcaa tgaaacgcgg ttgaaggcgg
     6661 tcgagctgga gggtgcgctc gccgcgctac gggcccggct cgcctacctg atcgcggagc
     6721 acgcatcatg agtgcgattc gaacactggg cgtcgtcgcg atcgccggcg cggtagtcct
     6781 ggccggcgct gccggcttcg gcgtcgccaa ctggcgcgcc gagcacagca tgcgcaacag
     6841 cgaccggccg ccctcggaca ggggctcggc tccgagcgcg gcgaaggaag gcgagcgcca
     6901 ggtgttgtac tggtacgacc ccatgtaccc gcaacacaag ttcgatcagc ccgggaagtc
     6961 ccctttcatg gacatgcagc tgatcccgaa gtacgcggac gaggtcagcg cctcgggggt
     7021 gaagatcgat cccacgctcg cgcagaatct cggcatgcgg ctcgcgacgg tggagcggca
     7081 gaccatgtcc accggagtga gcgcggtggc caccgtcggc ttcaacgagc gcgacgtcgc
     7141 catcgtgcag gcgcgcaccg gggggttcgt ggaacgggtc tacgcccgtg cgcccgggga
     7201 tgtcgttcga gccggcgccg cgcttgccga cgtgctggtc agcgagtggg ctggcgcgca
     7261 agctgagttt ctggcggtga aggccacggg agacgccgag ctgacggcgg ccgcgcgcga
     7321 gcgcctgcgg ctactcggca tgaccgaggc gcagatcgct caggtcgagc gatccgggca
     7381 gctcagcgcc gtgatgacca tcaccttccc gatcgcgggc gtgatccagg agctcggaat
     7441 ccgggcaggg atgtcggtga cgccgggcat gacgctcgcg cgcatcaacg ggttgcgtac
     7501 cgtatggctg gaagcggctg tccccgaagt gcatgcggcc atgctcgtcg ccggccgccg
     7561 agtcgaggcc cttttctccg cttacccggg cgaagtattc gaaggcaaga tcgcggcggt
     7621 gctgccggag gccgatcgcg acacgcgcac gctgcgcgtg cgcatggagt tcgccaatcg
     7681 cggccagcgg ctcaaggccg gcatgttcgc ccaggtcacc atcgccggcg aaagcaagga
     7741 agccttgatg gtgccggccg acgcaatcat ccgcaccggc aagcgggcgc tcgctttcgt
     7801 ctccgagcag ccgggacagt tcacgccggt ggaggtggag gtcgggcagg agatggacgg
     7861 caagctcact gtgctgagcg gcctcgaaga gggacagcag gtggccgtat cgggccagtt
     7921 cctgatcgat tccgaggcga gcttgtcggg gctgacgcag cgcatgggcg agggttccac
     7981 cgccgccggc gacacgaaca cgcaagcggc cgcagggccg atccacgagg gcatcggcac
     8041 ggtggaggag ctgtcgggca ccgcggtgac gctcaagcac ggccccatcg cgagcctgca
     8101 gtggggcccg atgaccatgc cgttcagctt gccgagcccc gacgtggcga aagacctgaa
     8161 agcgggcgac agcgtgcgct tccgcttccg gcaagtagag ggcaacttcg tcatcgagag
     8221 catcgagaaa accggaggcg gccgatgatc gagaagctca tccattggtc ggtgcacaac
     8281 cgttttctgg tgctgctcgc gacagtgttc gtcagcgcct ggggcgtgtg ggcgctgcgc
     8341 accacaccga tcgacgccct gcccgatctg tcggacgtgc aggtcatcat ccgcaccacc
     8401 tacccggggc aggcgccgca gatcgtcgaa aaccaggtga cctacccact cgccaccacg
     8461 atgctgtcgg tgccgggcgc gaagaccgtg cgcggctact cgttcttcgg cgattctttc
     8521 gtgtatgtgc tgttcgagga cggcaccgac ctgtactggg cgcgctcgcg cgtgctcgag
     8581 tacctgaacc aggtgcaggg gcggctgccg gcgaccgcca agacggcgct gggaccggac
     8641 gcgaccggcg tgggctggat ctaccagtac gcgttggtgg accggaccgg gcgccacgac
     8701 ctgtcgcagc tgcgggcgct gcaggactgg ttcctgcgct tcgagctgaa gagtctgccc
     8761 aacgtcgcgg aagtcgccag tgtgggcggc atggtgaagc agtaccaggt ggtgctcgac
     8821 ccgttcaagc tcgccgcctt cggcatcagc cacacccgag tgcgcgaggc gttggacgaa
     8881 gccaaccgcg aaacgggcgg ctcggtgctc gagctggccg aggcggagta catggtgcgt
     8941 gcgagcggtt acctgcgctc tctcgacgat ttccgcgccg tgccgctggc cgtcgtgggc
     9001 ggggtaccgg tcaagctggg cgacgtggcc atcgtccagc tcgggcccga gatgcggcgc
     9061 ggcatcgcgg agctggacgg cgaaggtgag gtcgcgggag gggtggtgct gctgcgctcg
     9121 ggcaagaacg cgcaggagac catcgccgcg gtgaaaacca agctcgccga gctgcagcgc
     9181 agcctgccca agggcgtgga gatcgtcccc acctacgatc gtagccacct gatccagcgc
     9241 gccgtcgaga acctgtcgca caagctgatg gaggaattcg cggtggtcgc gctggtgtgc
     9301 gtgctgttcc tgtggcacct gcgctcagcg ctggtggcga tcatctcgct gccgatcggg
     9361 gtgctgatcg ccttcgtggt gatgaagcat cagggcatca acgccaacat catgtcgctc
     9421 ggcggcatcg ccatcgccat cggtgcgatg gtggacgcgg cggtggtgat gatcgagaac
     9481 gcgcacaaga aggtcgaggc ctggcagcac gcacacccgg gccagcggct gcacggcgag
     9541 gagcactgga aggtcatgac ggaggcggcg gccgaagtgg gccccgcgct tttcttcagc
     9601 ctgctgatca tcaccttgtc gttcatcccg gtgttcactc tgcaggcgca ggagggccgt
     9661 ctgttcggcc cgctcgcgtt caccaaaacc tacgcgatgg cggcggcggc ggggctcgcc
     9721 gtgacgctgg tcccggtgct gatgggctat tggatccgcg ggcgcatccc cgaggagacg
     9781 aaaaatccgc tgaaccgggt gctgatcgcg gcctatcggc cgctgctcga ggccgtgctg
     9841 cgctttccca aagcaacgct ggccatcgcc atgctcctgt tcgcgagcat gctctggccg
     9901 ctcgcgcgct tgggcggcga gttcctgccg ccgctcgacg agggcgatct gctgtatatg
     9961 ccgtccgcct tgccggggct gtccgccgcc aaggcgagcc agctgctcca gcaaaccgat
    10021 cgcatgatca agaccgtgcc cgaagtcgag cgggtcttcg gcaaagcggg ccgcgccgag
    10081 acggccaccg accccgcgcc gctcgagatg ttcgagacca ccatccagct caagccgcgc
    10141 gaggagtggc gacccggcat gaccccggag aggctcgccg atgagctgga ccgggtggtc
    10201 agggtgccgg gcctcgccaa catctggatc ccgccgatcc gcaaccgcat cgacatgctc
    10261 gccaccggca tcaagagccc gatcggcgtg aaagtatcgg gcaccgacct ggcggcgatc
    10321 gaccgcgtcg cggcgcaggt ggagcaggcg gcgaaaggcg tccccggggt gagctccgca
    10381 ctggccgagc gcctcgccgg cggccgctat atcgatgtgc agatcgaccg ccaggcggct
    10441 gcgcgctacg ggctgaacat cgccgacgtg cagtcggtga tcgcctccgc tatcggcggg
    10501 gagaatatcg gcgaaacggt cgaggggctg gcgcgttttc ccatcaacct gcgctatccg
    10561 cgcgagctgc gggactcgcc cgagaagctg cgccagttgc ccatcgtcac cccgattgga
    10621 cagcagatca cgctgggcac cgtcgcgcgc gtggagacca ccgacggccc gcctatgctg
    10681 aagagcgaga atgcgcgcct gtcgggctgg gtctacattg acgtcagagg ccgcgacctc
    10741 gcctcggtcg tcgccgacct gcgccgagcc gtcgtccgcg aggtcacccc cgcgcccggc
    10801 atcggcatcg cctactccgg gcagttcgag ttcctggagc gggccaacgc caggctgaag
    10861 gtggtggtgc ccgccacgct cgtgatcatc ttcgtgctgc tgtacctgac cttcaagcgc
    10921 ttcgacgagg cgctgctgat catggtcgcc ctgcccttcg ccctcaccgg gggcgtgtgg
    10981 ttcctctatc tgctcggcta caacctgtcg atcgccaccg gcgtgggctt catcgcgctg
    11041 gccggcgtgg cggccgagtt cggtgtggtg atgctgctgt atctcaagca cgcgctccag
    11101 gtgcgcaccg gatcggacgc agcggcgccg atcgaggtgc tcgacgacgc catccgcgaa
    11161 ggcgcagtgc tgcgggtgcg gcccaaggcg atgacggtag cggtgatcct ggcggggctg
    11221 ctgcccatcg tctggggcgg tggcaccggg tcggaggtca tgagccgcat cgcggcccct
    11281 atgctcggcg gcatggtcac ggcgccgctg ctgtcgatgt tcgtcatccc agcggcatac
    11341 aagctgatgc gccgtcaacg cctgcgcgcg ggtgccgacg cgactgagcc gctggaggca
    11401 cgggcgctgc gcttcgcggc acgccgctag cctggcctgt ccagcgggcc cgcgttatcc
    11461 tgagcctcgc accagagcgc accgtgaacg gaaagagggg gcccccacag gtatgcaagc
    11521 actgaactgg cgccacacgc accactacgt ccagggcgaa tccgtcgagg tgggagacgt
    11581 cccgcgccac gtcgtcggca tcatccgcgg cagcgggctg ggcttcttcg acgacggcgc
    11641 ggtcgcgacg cacagcatcg tcatcgtgca cgacttcacc gacgggaccg gaccccacag
    11701 cggctacgtc acctacgcat tcgaggacgg cgccacgctg tcgctgtcct accgcggcac
    11761 gagcacgaca ccggcgggac agaacagcac cctgatcgag ggcaagttca ctttcctgcg
    11821 cggcagcggg cgcttctcgg gcgtcgcggg aagcggcgac taccggggca agcggctgat
    11881 cccggcggcc ggcggcggcg aggtgtatct cgacttcgtc ggaacgcgtt cgcgctagtc
    11941 tagtaccctc acccccccct ctcccgcagg gggagagggg gtgcaaaagc ccgctagtcg
    12001 tcgatgtagg cgaccgcttc gatctccacc ttgagccgcg gatcgacgag cttgctcacc
    12061 tcgaccagcg tcgaggccgg gcggttgtcc ccgaagtagt cgatgcgctg cggattgatc
    12121 agcgggcgct cggagatgtc ggtcatgaaa accgtgacct tgaccacgtg cctgggcttc
    12181 gcgccgacct ggcgcaggca cgcgtccacg ctcgcgagcg ccacccggaa ctgctccacg
    12241 gtggtctcgg ggatcgagcc gtcgggcttg atacccaccg atcccgacac ccacacgtgg
    12301 cggtcggcgc gcacgacgtg gcagtagtgg ctgacgggcg ccggctgccc gggaattacg
    12361 tggcgttcga tcatttcagg cctccttttc gttgactacc gtatcaagcc gatgtccctg
    12421 aagtagcgcg cgacgcccgg gtgcaacagc tcgggccgat gggcggcggc gagcgtattc
    12481 gccggcgtcg tgtcgcgcgc ttgcgccagg cgctgtccga gcgccgcctg gccgaggtgc
    12541 agggcccgcg caaaccgata cgcggcctca tccggcaagc cgggccgggc cagaatcagg
    12601 ctccatgtgc cgacggtggt gatggcctga tgctggcccg ggtaggactg tgcggccacc
    12661 gtcatagtct gcaggaacgg atgcttggcg cggatgcggg cgagatcatc cggctcgggg
    12721 ccgatgaatc gcgccgcttg cgggccgcgc gccacggcgg cgaaacccgg ccagccgacg
    12781 ccggcgcccc agagtgcggc cgcctcgccg cgcagcacca tcggcggacc gtccgcgacg
    12841 cgctccagca gcaccgcgtc gaaatccctg cgcagatcca gaccgaggcc atccaggacg
    12901 tagcgcgcca gcaccaccag tccggaggtc gcggcgccga agacgacgcg ttcgccgcgc
    12961 aggtcccgga tggtccggta cctcgagtcc ccgcggacca cgaacatgcc cgggctcgga
    13021 tacatgacgg cgaccaggcg cagatcggcg ggtgcgcgcc cgatgccggt catcgcttca
    13081 tacgcggtcg taccttcgac caaggccaga tcgagccttc cggcttcgag caggggaatg
    13141 ttttcggtgc tgcccccggt cgcctggagc tggatctgca gcccggggtc ggtttcagaa
    13201 agagtgtccg cgacagcctg tccgtacacc gcaaaccccc cgcccgcggt ggcggtcccg
    13261 agaaccagag gagcgacctc cgctccaacg gaagcggggg cgaggatcat ggtgcagagg
    13321 agaagcagcg ccgggggtcg gacttgcaca gcgttcatct gtacgcttgc gaacaggcgc
    13381 tgtcaatccg gcccttgggt agcaagtcgt tatcccggcg gacgcgccac gcggagcagc
    13441 ttcgtcgagc gatggtgaag gagccagatc cgatcgaatg gcgtgtgccg gtgggaaccg
    13501 agaccgtcgt cgcggtgtac gaacccgccg ccgcgagcgg cggcgcggcc ctcttcgtct
    13561 gcgcgcatgg cgccggcggc cacaggaacg attccggcat gcagaggctc gccgagcaac
    13621 tgcgcgggcg cgggatcgat atggtccggt tcaacttcct gtaccgcgag aagggggcgc
    13681 gcgctccgga cccgatgcct cgtctcatgg actgcttcgc agccgtggcc tcgcacgctc
    13741 gccgggagac acaggccgat ctgctcatca tcggcggccg ttccatgggc ggacgcgcgg
    13801 cgtcgatgct cgcggcgcag ggattcgcgt gcgacgcgct cctgctgctc gcctatccgc
    13861 tgcatcccgc cggcaagccc gagcaactgc gcgacgctca cctggcgcgg atcaagatgc
    13921 cggtgctgtg cttgaacggc acgcgcgaca atctctgccg acgcgatctg atggacacga
    13981 tcgtggccgg attacctgat tcatggacca tgcactggct ggaagctgcc gaccacggat
    14041 tccatgtgcc tcgaacgtcg gggaggtcgg acagcgatgt tctggccgag atcggggagg
    14101 tgatcgagcg ctgggtgttg cacggagcac tctgacaggg atgcgcaagc catcgtagac
    14161 gaccagctct tccgaaccac cagctatgcg gaacagcttt ctcggcggat acattactgg
    14221 gcccggaagc actccgccaa ctggcgctcc agtgatgttt cggcgaaggg acaggagcgg
    14281 tcttctacgc tgcgctgtcg gcgagccgca aggcctccac gccgtcgagc acgtgctcgg
    14341 catcatggcc aagttccctc agccggtcgc gcaggatgcg gccgatgccg ggatcgtcgt
    14401 cggcgatgac gattctgctc atcatgcgcg caatgcgggc aggagcctac gatttcatcg
    14461 ccaaaccatt cgacgccgcg acgatacagc tggtcgtgaa gcgcgcgctg gagacacgcg
    14521 gcctgaaaac gcaagttcac agcttgcgca aagagctggg ccgcaagcac ctgtgggtac
    14581 gaggtgcaga tcccggcatg gcccgcatcg ccgaaactgt ggagcgcatc gcaccgagca
    14641 cggccactgt gctgctgttg ggcgaaaccg gcaccggcaa ggaggtgatt gcccgcgcca
    14701 tccacctgca aagcccgcgc cgcgagcagc cgttcgttgc cgtcaattgc gcggtgctga
    14761 aaggcgagtt gctcgagagc gagctgttcg gccacgagaa gggcgccttc accggcgccg
    14821 accgggcacg gccgggccga gtcgaagcgg cccgtggcgg aacgctgttc ctggacgaga
    14881 tcgcggagct gccgccgaac ctgcaagcca agcttctgcg cgtgctgcag gaaaaggagt
    14941 acgaacgcct gggcagcgac cgcacgcagc gcgccgacat tcgcctcgtt gccgccacca
    15001 atcgcgacct tcccgccgcc atccaggccg gcgctttccg cgaagacctg tactaccggc
    15061 tcaaggtgat cagcatcaag atcccgccgc tgcgggagcg cccgagcgat attctggcgc
    15121 tggcggaatg gctgctcggc cagcatgcgg ccgaatcggg acgcactcca ccgcgcttgt
    15181 ccgaggaggt cgaacaagcg ttgctgcggt actcatggcc cggcaatgtg cgggagttgt
    15241 cgaacgtcat ggagcgctgc gtactgctgg cttgcgatac cgtcggcctg tgcgatcttc
    15301 ccgaggaatt gatgggcgga gccgcagcgc cgggtgcggt gacgaaggtc gaggacctgt
    15361 tcgccctgga gtacaacgct gccgtaacgg ccgcccggcg cttgatcgtc gccaaagcat
    15421 tggagcaaac cggtggccac cagacccggg ccgcggagcg gctgggcgtc acccagccct
    15481 acctgtcgcg cttggttaag cagttgggga tccggcgcga cgaccagcac tgaccgcctt
    15541 ggggcggccg cgcggcggat ggcgacgcgc ggcgccgggg gcgaccgcgt ataccgatgc
    15601 cgcctgttcc agacgcaaag taaacgggct gcgaaccgca acgctcccga aaacaccgcc
    15661 gtcttggaga tcttcggtca acaacagcgc gcacccctgc aactgtgccg caccgaccac
    15721 caagctgtcc caccagccga gccgatatcg aagctcgatt tcccggcccc gccgcagcag
    15781 cgcgtcgtcc acaggctgtg gattccaggt cagcagcgct tgcacgtcgt cccacgcctc
    15841 ttcggcgggg aggcccgggc tgagtttccg agtcacattc acgtagtact cggagagaac
    15901 ctgcacgctg gtgcgcccga tctgctcgcg ccagaggtgt tcgagccagg cggcggcacg
    15961 cggctgcttc gccgtttcac cggcatcgcg ggcatagagg aagacattgg tgtcaacgaa
    16021 cacgggagcg atcatgcgtc tcctcgcgtg tcgcatagcg cccgccggca cgcttcaacc
    16081 ggacgggctt cttcgccagg aagcgtcgca tcgcctcatc gtactcgtgg gattcccgca
    16141 ttctctgctg cagcatctcg ccgaccatcc gcgacacgct cgtgttgtgc ttggctgcgt
    16201 agactcgggc ccaggtcgcg gttttttcgt cgagcgtgat ggtgacgttc ttcatggcag
    16261 ggctactttg tgaaatacac gacggcagtg taacacgaat atcgtgtcat ttcgtgccga
    16321 cgctacaagc catcagagat acccgcctgg gggccgctct acgatgtttg gctgatcttt
    16381 gcgagcgcca tgtacagccc cgcgtgctgc ggctcggcgc gatggcgctc ggcgatggcg
    16441 aacagctcgc gtacgttggc ggcgatcttc tcgcgcgtca gggacttggc ttcctcggcc
    16501 ttgtcggcgg actgcgaggt cagttcgacg aaggcggcga tgacgccgcc cgggttggcg
    16561 atgaaatcgg gcaccagggc gacgccgcgg gcgtgcaagc tcgtgcgcgc agcctcggag
    16621 acgggtgcgt tggcgccctc gcagacgtag cgcgcctgga cgcgcggcgc attgtgctcg
    16681 gtgatcacgt tctgcacggc gcacggaaac aggatgtcgg tctctaggta cagaacctcc
    16741 tgcgggtcgc gggaaacgca cgtgccttcg ttcgccagca gcggcttggc ggtttcgctg
    16801 tcctgtcttg cgagcgcccc atgcaggtcg gcggaaggtg gcgcatcgaa ggtccaggtc
    16861 cctccgtatc ggggatcgcc cagcgccgcc aggcgcgctc ccgtctcgct gaaataacgc
    16921 agcaccgcgg cacccattgc gcccatgccc tgaacggcga agctcgcttg cgccgggctt
    16981 tcgcccttga cctccagcat ggtcccgccg gcaaccgcca cgcccaaccc ggcgatgccc
    17041 tcgcggtcgt agtcggtgcc gcccatgtgc accggctttc cggtaaagca gcgggtcgat
    17101 ttcagctcgt cgcacgccca caatgcatgc tccggacgtg cgccgatgtc gaacccgaac
    17161 ccgccgaacg ggccgccatt ctcgtacagc aaaggagcgc agaggcggac gaaacggcga
    17221 tagcgctcct cgaaaccggg tgcggatgga tcgagcttga ggcccgactt ggcgccgccc
    17281 aaaggcagcc ccgccgccgc gttcttgagc gccatggtgc gcgccagcat cttcacctcg
    17341 tccagagatg cgttcggggt gatgcgcgtg ccgccttttg cgcaacccgg cagcgggccg
    17401 cccgcgctga tgccggtgtt ccacaccacc acgtagcctt cgacgtccag agccggatcg
    17461 cgcagcgtga cttccagctc gggctgcatc gcttcgaagt gctgtttcag gcgctgaagc
    17521 tcggcgtaga aagcagcccg gtcggtgcgg gcagttgccg ctgcgccgcc catcggcgcg
    17581 ttccggtcgg cgggcgctgt cgacctacct ctcggcatac gcgccgcgct cgatctcgtc
    17641 gagcagcgcc ggcccgttgg gcttccagcc cagctcacgg cgcgcccgca ccgcgcgcac
    17701 ccggctgttg gagccgaagc tgtagttggc ggccgcttcg cccagctcgg ccaccgcctc
    17761 ctgcatcgac aggggctgcg tgcgcccgcc gaagcccagc atgcggctga tggccgcggc
    17821 gacctcgcgg tacgagttct cgccgttctc ggcatagtag aaagcgcccg ccggcgcctt
    17881 ctgcaacgcc agcaggtaca gcgacaccag gtcgtcgatg tggacgttgg accagatgtt
    17941 ctcgcccggc cccacgtgcc ggcctacgcc gtactttttc gccagcgcga tcaggcgggg
    18001 gacctggacg ctgtcctgat gggcgccgcg cccgcgcccg tagatcatgg tcgggcacat
    18061 gaccacggcg cgtacgccgt tccccgccgc ggccaacacc agccggt
//
LOCUS       DASPGE010000327         3533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1256888, whole genome shotgun sequence.
ACCESSION   DASPGE010000327 DASPGE010000000
VERSION     DASPGE010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256888"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VD791_10535"
     CDS             complement(<1..1220)
                     /locus_tag="VD791_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXV08456.1"
                     /translation="MPVPTGKPVCTRLFALQAAVLGLAGLLLTVCVAPAPAQETPFPP
                     ALDLSDEEKAWLSTQRVIRIGAETNYAPYEFQDSRGRFTGVVADYLELIRQRLGMRFE
                     VHQLLDFEAVENKVQKRELDVILALPRTTEREESLAFTKPYLHYVNVIVTRDDFGFVT
                     GLRDFADERVAVVAGKSSQRLIAKIYPNFNVTTYSDLLDALMAVSTGKEDGLLDDIFP
                     IVYNIRHRQISNLKIATPVEKALQPKGFSIAVRNDWPELVGILDKVLLTITQEEHRDI
                     SEKWLSVRYESKIDYRAIWTAVAVFSAILLAAIFWIRMLSRQRKALMAARAEAEAANR
                     AKDEFLASMSHELRTPLHAILGYAELLREGALAGAARQEALATIRGSGRHLLTLINDL
                     LDLSRIRAGHLELNL"
     gene            1342..2787
                     /locus_tag="VD791_10540"
     CDS             1342..2787
                     /locus_tag="VD791_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXV08457.1"
                     /translation="MTSEELSYLPAHALAQAIRERQISPVEAVRTVLDRAQRAQPALN
                     AFITLCPEQALAAAREAEAAVLRGEAAGPLAGVPFSVKDLVNTAGVRTTYGSLIFERH
                     VPEADAVAVARLKAAGAILIGKTTTPEFGQKGLTEAPLFGRTRNAWRADRTSGGSSGG
                     AAVAVACGLAPLAVGTDGGGSTRIPAACNGVVGLKQSLGVVPHDRAQDAFGNISYITP
                     MARTVADTALMLDAMAGAHPSDPHTLNRLRPRPGYFAAVGAGGDLKGLKVAWRPRLGN
                     TVVAEQVLASCAAAARAFADFGASVLEIPDDFDNAEELWLIVNGCYRLAQFGEHLAQH
                     RQRMDPTLVAQLDRAAKYSAQELYRAIFLRTELYRQVQAWFDRADIVITPTLARTALP
                     IDHDFFAPVEIDGRPVDTMRRAWYPYTLPFNMTGNPALSIPCGFDGDGLPVGLQLVGR
                     IGEDALLLRAAALFEAAHPWAHRRPPLPESA"
     gene            complement(2803..3531)
                     /locus_tag="VD791_10545"
     CDS             complement(2803..3531)
                     /locus_tag="VD791_10545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV08458.1"
                     /translation="MTGAGTGIGKAVSLAFLRDGYRVALAGRRKEPLEQTVAESGAEA
                     GRALAVPTDVADPQSVKALFARVKEKFGRLDVLFNNAGINAAGLLEDLTVEQWKSVVD
                     INLTGPFLCTQEAFKLMKSQTPRGGRIINNGSISAHAPRPNSAPYTSTKHAITGLTKC
                     TSLDGRKYDIACGQIDIGNALTELSAKMTKGVPQANGEIAVEPMMDVKDVANAVLHMA
                     SLPLDTNVQFMTIMATKMPFVGRG"
BASE COUNT          517 a         1172 c         1250 g          594 t
ORIGIN      
        1 aggttgagct ctaggtggcc ggcgcggata cgcgacagat cgagcaggtc gttgatcagg
       61 gtcagcaggt gacgtccgct gccgcggatt gtggcgagcg cctcctggcg cgccgcgccg
      121 gccagcgcgc cctcgcgcag cagctcggcg tatcccagga tggcgtgcag cggcgtgcgc
      181 agctcgtggc tcatgctcgc caggaactcg tccttggcgc ggttggcggc ctccgcttcg
      241 gcgcgcgcgg ccatcagcgc cttgcgctgg cggctcagca tgcggatcca gaagatggct
      301 gccaacagga tcgccgagaa caccgccacc gctgtccaga tcgcccggta gtcgatcttg
      361 ctctcgtagc gcaccgacag ccacttctcc gagatgtcgc gatgctcttc ctgggtgatg
      421 gtgagcagca ccttgtccag gatgccgacc agctcgggcc agtcgttgcg cactgcgatg
      481 gagaatccct tgggctggag cgccttctcc accggcgtgg cgatcttgag attgctgatc
      541 tggcggtggc ggatgttgta gacgatcggg aagatgtcgt cgagcagacc gtcttccttg
      601 ccggtggaca ccgccatgag cgcgtcgagc aggtcggaat aggtggtgac gttgaagttc
      661 gggtagatct tggcgatcag gcgctgcgaa gacttgccgg cgaccaccgc tacacgctcg
      721 tcggcgaagt cgcgcagtcc ggtgacgaag ccgaaatcgt cgcgcgtgac gatgacgttg
      781 acgtagtgca gatatggctt ggtgaaggcc agggactcct cgcgctcggt ggtgcgtggc
      841 agcgccagga tgacgtccag ctcccgtttc tgcaccttgt tctcgaccgc ctcgaaatcg
      901 agcagctgat gcacctcgaa gcgcatgccc aggcgctggc ggatgagctc caggtagtcg
      961 gccaccacgc cggtaaagcg cccgcggctg tcctggaact cgtagggggc gtagttggtc
     1021 tccgccccga tgcggatcac gcgctgcgtg gacagccagg ccttctcctc gtcgctgaga
     1081 tcgagcgccg gcgggaaggg cgtctcctgg gccggggcgg gcgccacgca caccgtcagc
     1141 agcagccccg ccaggcccag cacggcggcc tgcagggcga agagccgtgt gcaaacgggt
     1201 tttccggtcg gtaccggcat gtgcgcaaat gccttcaagg gggcgggcgg gttcctgccg
     1261 tggcgcctcc cgtagtgtaa tgcttcggcg ggatagcgcg taagctcatg catgcgggag
     1321 gccccccaag atgagatcgg catgaccagc gaagaactga gctatctgcc ggcccatgcg
     1381 ctggcgcagg cgatccggga gcgccagatc tccccggtgg aggcggtgcg cacggtcctc
     1441 gaccgagcac agcgggcaca gcccgcgttg aatgccttca tcacgttgtg cccggagcag
     1501 gccctcgccg ctgcgcgcga ggcggaggcc gcggtcctgc gcggcgaggc cgccgggccg
     1561 ctggcgggcg tgccgttctc ggtgaaggat ctggtgaaca ccgcgggcgt gcgcaccacc
     1621 tacggctcgc tgatcttcga gcggcatgtg ccggaggcag acgccgtggc ggtcgcgcgc
     1681 ctgaaagccg ccggggcgat tctcatcggc aagaccacca ccccggagtt cggccagaag
     1741 ggtctgaccg aagcgccgct gttcggccgc acgcgcaatg catggcgcgc cgatcggacc
     1801 tcggggggct ccagcggcgg tgcggccgtg gccgtggcct gcgggctcgc gccgctggcg
     1861 gtgggcaccg acggcggcgg ttccacccgc atcccggccg cctgcaacgg cgtggtcggg
     1921 ttgaagcaga gcctgggcgt ggtgccacac gatcgggcgc aggacgcctt cggcaacatc
     1981 tcctacatca cgccgatggc gcgcaccgtg gccgataccg cgctcatgct cgacgcgatg
     2041 gcgggcgcgc atccgagcga tccgcatacg ctcaaccggc tgcggccgcg gccggggtac
     2101 ttcgcggccg tcggcgcggg cggcgatctc aaagggctga aggtcgcctg gcgcccgcga
     2161 ctcggcaaca ccgtggtcgc cgagcaggtg ctggcttcgt gcgcggccgc cgcccgggca
     2221 ttcgcggact tcggcgcgag cgtgctcgag atacccgacg acttcgacaa cgccgaggag
     2281 ctgtggctga tcgtgaacgg ctgctatcgg ctggcgcagt tcggcgagca cctcgcgcag
     2341 caccggcagc gcatggaccc caccctggtg gcgcagctcg accgcgctgc gaagtactcg
     2401 gcgcaagagc tgtaccgcgc gatcttcctg cgcaccgagc tgtatcggca ggtgcaggcc
     2461 tggttcgacc gggccgatat cgtgatcacg ccgacgctgg cgcgcacggc gctgccgatc
     2521 gatcacgatt tcttcgcgcc ggtggagatc gatgggcgcc cggtggatac gatgcggcgc
     2581 gcctggtatc cctacacgct gccgttcaac atgaccggca atccggcgct gagcattccc
     2641 tgcggcttcg acggcgacgg cctgccggtg ggcctgcagc tcgtcggccg tatcggcgag
     2701 gacgcgctgc tgttgcgcgc ggcggcgctg ttcgaggcgg cgcacccgtg ggcgcaccgg
     2761 cggccgcctc tcccggaaag cgcgtgaggc gcggcgggcc gtttacccgc gcccgacgaa
     2821 cggcatcttc gtcgccatga tcgtcatgaa ctgcacgttg gtgtccagcg gcaggctggc
     2881 catgtgcagc accgcgttgg cgacgtcctt gacgtccatc atcggctcca ccgcgatctc
     2941 cccgttggct tgcggcacgc ccttggtcat cttcgccgaa agctcggtca gcgcattgcc
     3001 gatgtcgatc tggccgcagg cgatgtcgta cttgcggcca tccagcgagg tgcacttggt
     3061 caggccggtg atggcgtgct tggtggaggt gtagggcgcc gagttcggcc gcggcgcgtg
     3121 ggccgagatc gaaccgttgt tgatgatgcg tcccccgcgc ggcgtctggc tcttcatgag
     3181 cttgaaggcc tcctgggtgc acaggaaggg gccggtgagg ttgatgtcca ccaccgactt
     3241 ccattgctcg acagtcaggt cctcgagcaa tccggccgcg ttgatgccgg cgttgttgaa
     3301 cagcacgtcg agccgcccga atttctcctt gaccctggcg aacagcgcct tcaccgattg
     3361 cggatcggcc acgtcggtgg gcaccgccag cgcccgcccg gcctccgcgc cggattcggc
     3421 gacggtctgc tccaggggct ccttccggcg tccggccagc gccacccgat agccgtcgcg
     3481 caggaaggcc agggataccg ccttgccgat gccggtcccc gcgccggtga tca
//
LOCUS       DASPGE010000328         6408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1022013, whole genome shotgun sequence.
ACCESSION   DASPGE010000328 DASPGE010000000
VERSION     DASPGE010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022013"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2142
                     /locus_tag="VD791_10550"
     CDS             <1..2142
                     /locus_tag="VD791_10550"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HXV08459.1"
                     /translation="VAAACDAIVADPANAREYTSRGNLVAVVTNGTAVLGLGNIGPLA
                     GKPVMEGKACLFKKFAGIDVFDIELNERDPEKLVDIIAALEPTLGGINLEDIKAPECF
                     YVERKLRDRLKIPVFHDDQHGTAIIVGAAVKNALRVVGKEIGKVKLVCSGAGASAIAC
                     LDLLVRLGLKPENVLVCDSKGVIYPGREANMEENKARYARKTAARSLADAIPGADIFL
                     GLSGPGVMTQAMVRDMAAKPLVLALANPTPEILPEDVKAVRSDAIIATGRSDYPNQVN
                     NVLCFPFIFRGALDVGASRITEEMKLACVHAIADLAQAEQSDVVAMAYEGQQLSFGPE
                     YIIPKPFDPRLIIKIAPAVAKAAMDSGVATRPIADFEAYRQQLSNFVYHSGFVMKPVF
                     ARAKQSPKRVIYAEGEDERVLRAVQVVVDEQLARPVLIGRPEVIDMRLQKFGLRIRPG
                     RDFELVNPESDPRYREVWTEYYKLMGRRGVSPDDARTHVRQSATLIGAMLLHRGDADA
                     LLCGTFGRHKDHLRHVRNVIGLKRGASLLAAMNVLLLPRRTLFISDTYVNEDPSAEQL
                     AEIAVMAAEEVRRFGLTPKVALLSHSNFGTDDSPSALKMSRARELVAQRAPDLEVEGE
                     MHGDAALSQEIRNRVYPDSRLKGQANLLIMPNLDAANISFNLLKMTGGEGVTIGPILL
                     GAARPVHILTPSATVRRLVNMTALAAVDAVA"
     gene            complement(2235..3350)
                     /locus_tag="VD791_10555"
     CDS             complement(2235..3350)
                     /locus_tag="VD791_10555"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HXV08460.1"
                     /translation="MPPLSGIRVLDFSTLLPGPMATLLLAEAGAEVLKIERPQTGEEL
                     RNFRPRFGDTSLSFALLNRGKRSIALDLKADDALARLRPLLESADVLVEQFRPGVMER
                     LGLGYEAVHAINPRIVYCSITGYGQEGPKAQVAGHDLNYQADAGLLGIAAGSDGAPVV
                     PGALIADIAGGSYPAVINILLALRQREHTGQGCRLDIAMTDGLLPFLYSAIGIGAATG
                     QWPRPGGELLSGGSPRYQIYTTRDGRYLATAALEDKFWGNFCDAIDLSAALRDDGKDP
                     QATIRAVRDIVRSRTAQQWTEHFAGRDVCCNLMQGLEQAVQDPQFRARGVFRRSVVND
                     DHDGLPAVSVPIAEPLRDPRTELRYPQLGEANDLLRP"
     gene            3518..4363
                     /locus_tag="VD791_10560"
     CDS             3518..4363
                     /locus_tag="VD791_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXV08461.1"
                     /translation="MPTVQETVLGNSALQEIEEISAAPLALDDIWRGIEADQFLPHFQ
                     PKVSLRGMELAGVEALMRWQHPERGLLSAGAFLPLIADNFLFDELTALMLEKSIAQSR
                     RWQNDKVNVPVSVNLSPDLLRDTGIAERIETKALRHGLPPHRLIIEVPESAIAHDIAE
                     ALDNLVRLRVKGFGLAIDDFGTGHCDRNQLERIPASEMKIDRMMLAGAPGRPALKALL
                     HQAIELARDLNLNSVAEGVESQEEWDLVNELGCDMAQGYFIARPMAGDDLVTWLHAWT
                     SDPFV"
     gene            complement(4417..5232)
                     /locus_tag="VD791_10565"
     CDS             complement(4417..5232)
                     /locus_tag="VD791_10565"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HXV08462.1"
                     /translation="MDQLSTLGPRLAIGGTGELFGWDDSRVLKLYWHGASMDAAEREA
                     ERTRAAHAAGAPAPEVFDVIAVDERPGVVFERVEGPSMLQAVGDDPGKAELFAQQLAA
                     LHAQLHAHAGIGLPPQREHLIRRINLGPLPARNKPLILAAMRRLADGEALCHGDFHPG
                     NVLMTKAGPRIIDWFDATAGDPGADFARTCLLLQYSRLPGTANASANRVMEALRERFL
                     DAYIECYRTLRPGTAETLVEWFLPVAGARIAEPIPAQERTTLLRVIGSLLPQA"
     gene            5395..>6408
                     /locus_tag="VD791_10570"
     CDS             5395..>6408
                     /locus_tag="VD791_10570"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HXV08463.1"
                     /translation="MNLRVALLRGYTGAYRAISGFLARKLSNNFYLHLAGLFSLFALA
                     DVLFLHQIVDMRQKSYDLVVKNRLLKPTPDPDIVIVDIDEKSLAAMASEYGRWPWPRQ
                     VLGEFLEHVAAQQPAGVVFDILFSDPDVFNPDSDAYFNEALAGSDRAFLAYLRLPAKD
                     DGLSELRPEKIPGAVKTPAAREGATVAVVLPFFPAALEGGRLGAINVEPDKDGVVRQY
                     PVSIEAHGWQLPSMPMRVAQAQGWSIPARQSILLNWRGKPFSYRYASFSDVYADMLSK
                     EKTRPADEFKGKMVLIGTTAAGLGDIKATSVAKQFPGVEILATAIDNLKHDDFIRVPT
                     RRWP"
BASE COUNT         1031 a         2144 c         2245 g          988 t
ORIGIN      
        1 gtcgccgctg catgcgatgc gatcgtcgca gatccggcca atgcgcgcga atacacttca
       61 cgcggtaatc tcgtcgcggt cgtcacgaac ggcacggccg tgctcggtct gggcaacatc
      121 gggccgctgg cgggcaagcc ggtgatggag ggcaaggcct gcctgttcaa gaagttcgcc
      181 ggcatcgacg tgttcgacat cgagctgaac gagcgcgacc ccgagaagct ggtggacatc
      241 atcgccgcgc tggaacccac gctcggcggg atcaacctgg aggacatcaa ggcgccggaa
      301 tgcttctacg tcgagcgcaa gctgcgcgac cggctgaaga tcccggtgtt ccacgacgac
      361 cagcacggca ccgcgatcat cgtcggcgca gcggtgaaga acgcgctgcg ggtggtgggc
      421 aaggagatcg gcaaggtgaa gctggtttgc tcgggcgcgg gcgcctcggc catcgcctgc
      481 ctcgacctgc tggtgcggct gggcctcaag cccgagaacg tgctggtgtg cgacagcaag
      541 ggcgtcatct accctggccg cgaggcgaac atggaggaga acaaggcgcg ctacgcgcgc
      601 aagaccgcgg cgcgcagcct ggccgacgcc atccccggcg cggacatctt cctcgggctg
      661 tccggcccgg gcgtgatgac gcaggcgatg gtgcgcgaca tggcggccaa gccgctggtg
      721 ctcgcgctcg ccaatcccac gcccgaaatc ctgcccgagg acgtgaaagc ggtgcgctcg
      781 gacgcgatca tcgccaccgg ccgctcggac tatccgaacc aggtgaacaa cgtcctgtgc
      841 tttccgttca tcttccgcgg tgcgctggac gtgggcgcca gccgcatcac cgaggagatg
      901 aagctcgctt gcgtgcacgc catcgccgac ctggcgcagg ccgagcagtc ggacgtggtg
      961 gcgatggcct acgaagggca gcagctcagc tttgggccgg aatacatcat ccccaagccc
     1021 ttcgatccgc gcctgatcat aaaaatcgcg ccggcggtgg ccaaggcggc gatggacagc
     1081 ggcgtggcca cccgcccgat cgccgacttc gaggcctacc gccagcagct cagcaacttc
     1141 gtctatcact ccggcttcgt catgaagccg gtgttcgccc gcgccaagca gtcgcccaag
     1201 cgcgtgatct acgccgaggg cgaggacgag cgcgtgctgc gcgcggtgca ggtggtggtg
     1261 gacgagcagc tcgcccggcc ggtcctgatc ggccggccgg aggtgatcga catgcggctg
     1321 cagaagttcg gcctgcgcat ccggcccggg cgcgacttcg agctggtcaa cccggaaagc
     1381 gacccgcgct accgcgaggt gtggaccgag tactacaagc tcatggggcg gcgcggcgta
     1441 tcgcccgacg acgcgcgcac gcacgtgcgc caatccgcca cgctcatcgg cgccatgctg
     1501 ctgcaccggg gcgacgcgga cgcgctgctg tgcggcacct tcggccggca caaggaccat
     1561 ctgcgccacg tgcgcaacgt gatcgggctc aagcgcggcg ccagcctgct cgcggcgatg
     1621 aacgtgctgc tgctgccccg gcgcacgctg ttcatctccg acacttacgt caacgaggat
     1681 cccagcgccg agcagctcgc cgagatcgcg gtgatggcgg ccgaggaggt gaggcgcttc
     1741 ggtctgacgc cgaaggtcgc gctgctgtcg cattccaact tcggcaccga cgattcgccc
     1801 tccgcgctga agatgagccg ggcgcgcgag ctggtcgcgc agcgcgcccc cgacctggag
     1861 gtggagggcg agatgcacgg cgacgccgcg ctgtcgcagg agatccgcaa ccgcgtgtac
     1921 ccggactcgc gcctgaaggg ccaggccaat ctcctgatca tgcccaacct ggatgccgcc
     1981 aacatctcgt tcaacctgct caagatgacc ggcggcgaag gcgtcaccat cggcccgatc
     2041 ctgctcggtg cggcgcggcc ggtgcacatc ctcacgccgt cggcgaccgt gcggcggctg
     2101 gtgaacatga cagcgctggc cgcggtggac gcggtggcgt aggccgggtg gttctgcgcg
     2161 tgcacaccac accgccccgc cggctgcgcc ggcaccccgc ctcgtgagag gcggggagtt
     2221 ttctgattcg cgccttaagg gcgcagcaaa tcgttggctt cgcccagttg cggatagcgc
     2281 agctcggtgc gcggatcacg cagcggctcg gcgatgggca cgctcaccgc cggcaggccg
     2341 tcgtggtcgt cgttgacgac gctgcgccgg aacacgccgc gcgcccgaaa ctgcggatcc
     2401 tggaccgcct gctccagccc ctgcatcaag ttgcagcaca cgtcccggcc ggcgaaatgc
     2461 tcggtccatt gctgcgcggt gcgcgagcgg acgatgtcgc gcacggcgcg gatcgtcgct
     2521 tgcggatcct tgccgtcgtc gcgcagcgcg gcggacaagt cgatcgcgtc gcagaagttg
     2581 ccccagaact tgtcctcgag cgccgcggtg gcgagatagc gtccgtcccg ggtggtgtag
     2641 atctgatagc gcgggctgcc gccggacagc agttctcccc ccggccgcgg ccattgcccg
     2701 gtcgcggcac cgatgccgat cgccgaatac aggaagggca gcaacccgtc ggtcatggcg
     2761 atgtccagcc ggcatccttg accggtgtgc tcgcgctgcc gcagcgccag caggatgttg
     2821 atgacggcgg ggtaggagcc tccggcgatg tcggcgatca acgcgccggg caccaccggt
     2881 gcgccgtcgc tgcccgcggc gatgccgagc agccccgcgt cggcctggta gttgaggtcg
     2941 tgtccggcga cctgtgcctt gggcccctct tggccatacc cggtgatgga gcagtagacg
     3001 atgcgtggat tgatcgcgtg caccgcctcg tagccgaggc ccaggcgctc catcacgccc
     3061 ggccggaact gctccaccag cacgtcggcc gattcgagca gcggccgcag ccgcgccagc
     3121 gcgtcgtccg ccttgaggtc gagcgcgatg ctgcgcttgc cgcggttgag cagcgcgaag
     3181 gacaagctgg tgtcgccgaa gcgcgggcgg aagttccgca gttcctcgcc ggtctgcggg
     3241 cgctcgatct tgagcacttc ggcgcccgct tcggcgagca gcagcgtggc catggggccg
     3301 ggaaggagcg tgctgaagtc gagcacgcgg atgccggaaa ggggtggcat agcggttcct
     3361 ggatgagacg gccgcgattg tagaacgccc gcgcagggcg cgcgcgcggc gccctcaagt
     3421 cctgctcttg ggggacgaaa tttgaaccgt gccgggggtg ccggggtccg attcaatcag
     3481 ggggccacgg ctcgagccgc cccccgggga ggggccgatg ccgacagtgc aggaaaccgt
     3541 cttgggaaac agcgccctgc aggagatcga ggagatctcc gcggcgccgc tcgcgctcga
     3601 cgacatctgg cggggcatcg aggccgatca gttcctgccg cactttcagc ccaaggtgag
     3661 tctgcgcggc atggagctgg ccggcgtgga ggcgctgatg cgctggcagc accccgagcg
     3721 cgggctgctg agcgcgggcg cgttcttgcc gctgatcgcg gacaacttcc tgttcgacga
     3781 gctgaccgcg ctcatgctgg agaaatcgat cgcgcagagc cgtcgctggc agaacgacaa
     3841 ggtcaacgtg ccggtgtcgg tcaatctctc gccggacctg ctgcgcgaca cgggcatcgc
     3901 cgagcgcatc gagacgaagg cgctgcggca tggcctgccg ccgcaccggc tgatcatcga
     3961 agtcccggaa tcggccatcg cccacgacat cgccgaggcg ctggacaacc tggtgcggct
     4021 gcgcgtgaag ggcttcggcc tggcgatcga cgatttcggc accgggcact gcgaccgcaa
     4081 tcagctcgag cgcatccccg cgagcgagat gaagatcgac cgcatgatgc tggccggcgc
     4141 cccgggacgt ccggcgctca aggcgctgtt gcaccaggcg atcgagctgg cgcgcgactt
     4201 gaatctcaac tccgtcgccg agggggtcga gagccaggag gagtgggacc tggtcaacga
     4261 gctgggctgc gacatggcgc agggatactt catcgcacgc ccgatggcgg gcgacgatct
     4321 agtgacctgg ctgcacgcct ggaccagcga tccctttgtt tgatcgaccg ccggctttga
     4381 ctgacccctg gcgtttgatc gaccgccggc aggccgtcac gcctgcggca gcagcgagcc
     4441 gatcacgcgc agcagagtgg tgcgctcctg ggcgggaatc ggctcggcga tgcgcgcacc
     4501 ggctaccggc agaaaccatt cgaccagcgt ctccgcggtg ccggggcgca gcgtccgata
     4561 gcactcgatg taggcgtcca ggaaacgctc gcgcagcgct tccatcaccc gattagcgga
     4621 ggcgttggcg gtgccgggca ggcggctgta ctgcagcagc aggcaggtgc gcgcgaagtc
     4681 cgcgcctggg tcgccggcgg tcgcgtcgaa ccagtcgatg atgcgcgggc cggcttttgt
     4741 catcagcacg ttgccgggat ggaagtcgcc gtggcacagg gcctcgccgt cggccagccg
     4801 gcgcatggcg gccaggatca gcggcttgtt gcgcgccggc agcggcccca ggttgatgcg
     4861 acggatcaga tgctcgcgct gcggcggcag gccgataccc gcgtgcgcgt gcagttgcgc
     4921 atgcagcgcg gcgagctgct gcgcgaacag ctccgctttg ccgggatcgt cgccgacggc
     4981 ttgcagcatg gaagggccct ccacgcgctc gaacaccacc cccgggcgct cgtccaccgc
     5041 gatgacgtcg aacacctctg gcgcgggcgc gccggcggcg tgcgccgccc gcgtgcgctc
     5101 ggcctcgcgt tcggcggcat ccatggacgc gccgtgccag tacagcttca gcacgcggct
     5161 atcgtcccag ccgaacagct cgccggtccc gccgatggcg agccgggggc ccagagtgct
     5221 cagttggtcc atgatcgggt tgcttcggga tgggcgcgca tcataccgtg agcatggccg
     5281 gtcgcgcagc tttcgtgcca gcgtgcacgc cgctgaggac gtcttgcaca atgccgcagc
     5341 gattcgtcca ggcggcggag ccgggcgcag cgggcctgct agaatcgcgc tcagatgaat
     5401 ctccgagtcg ccttgttgcg gggctacacc ggcgcctacc gcgccatttc cggctttctc
     5461 gcccgcaagc tcagcaacaa cttctacctg catctggccg gtctgttctc gctgttcgcg
     5521 ctggccgacg tgctgttcct gcaccagatc gtggacatgc ggcagaagtc ctacgacctg
     5581 gtggtgaaga accgcctgct caagcccacc cccgatcccg acatcgtcat cgtcgacatc
     5641 gacgagaaga gcctggcggc gatggcgagc gagtacggcc gttggccctg gccgcggcag
     5701 gtgctgggcg agtttctgga gcacgtcgcc gcgcagcagc cggcgggggt ggtgttcgac
     5761 atcctgttca gcgacccgga tgtgttcaac ccggacagcg acgcctactt caacgaagcg
     5821 ctggccggca gcgatcgcgc atttctggct tatctgcggc tgccggcgaa ggacgacggc
     5881 ctgagcgaac tgcggccgga gaaaatcccc ggggcggtga agacgccggc ggcgcgcgag
     5941 ggcgcgaccg tggccgtggt gttgcctttc ttcccggcgg cgctcgaagg cgggcgcctg
     6001 ggtgcgatca acgtcgagcc ggacaaggac ggcgtggtgc gccaatatcc ggtgtcgatc
     6061 gaagcgcacg gctggcaact gccctcgatg ccgatgcgcg ttgcccaggc gcagggttgg
     6121 agcatcccgg cgcggcagag catcctgctc aactggcgcg gcaagccctt cagctatcgc
     6181 tacgcgagct tcagcgacgt ctacgccgac atgctgagca aggaaaagac gcgtccggcg
     6241 gacgagttca agggcaagat ggtgctgatc ggcaccaccg cggccgggct gggggacatc
     6301 aaggccacct ccgttgccaa gcagtttccc ggcgtggaaa tactggccac cgcgatcgac
     6361 aatctgaagc acgacgactt catccgcgtg cccaccaggc gctggccc
//
LOCUS       DASPGE010000329         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1022102, whole genome shotgun sequence.
ACCESSION   DASPGE010000329 DASPGE010000000
VERSION     DASPGE010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022102"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VD791_10575"
     CDS             <1..808
                     /locus_tag="VD791_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003273982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HXV08464.1"
                     /translation="FATPRFHHLSELYGYLAKQFTVFGQHIHIGCDSGDRAIGLIHAM
                     SRYIPHFIALSAASPFLQGVDTAFESARLNSVNAFPLSGHLPPVRNWAEFEAYFDSMR
                     ELKVVSSMKDFYWDIRPKPEYGTVEVRVCDTPLTVERAAALAAFGQALARCLLDGGTP
                     RVDPEAYRVYGYNRFLACRFGLEAELVDFERREKLALRDDLLDTLRLARGFASDTSGA
                     EALDKLAEDVQRNESDSAWLRETHRRKGSLSDVVRAQCQVWQSGQRVREP"
     gene            881..1807
                     /locus_tag="VD791_10580"
     CDS             881..1807
                     /locus_tag="VD791_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HXV08465.1"
                     /translation="MNDWLTLALGVAAAGAGGELFVRGAVGLARRMRISPGIIGATVA
                     AFATSSPELSVAVGSALAQRPQISLGDALGSNVVNVGLILGLALVISGTRSPRDAVRR
                     DFPAAIAAPVLTAACLLDGVLSRPEAMLLFAGFILWFLAAIQEARRQRSAAPAVLGER
                     LAGRAIAEGALGLIMLIAAGRLIVLGAKGIALSAGIGEFVIGATVVAMGTSVPELATA
                     IVSKLRGHDEVGLGTILGSNIFNGLFVVPLAAAIFPIAIALREVAGALIFGLLAVALT
                     FPAAGGWIGRGRGVLLLALFAAFLFSTLRAGL"
     gene            1946..2236
                     /locus_tag="VD791_10585"
     CDS             1946..2236
                     /locus_tag="VD791_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08466.1"
                     /translation="MGMMHYRPFARKARAILTERDYRAARAVLLASARMALLREEEER
                     VEALLRELAHYDSNPAEFEPGPATDPVQSGPARGFDGNGPGRRWSDRHSREG"
     gene            complement(2286..2801)
                     /locus_tag="VD791_10590"
     CDS             complement(2286..2801)
                     /locus_tag="VD791_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HXV08467.1"
                     /translation="MRNHWSLRPFRRWLRHRSLWSLEQRPVARGVAIGLFFGILLPVA
                     QILFAAVAAVLLRANLAVAAASTLVTNPLTFPFVYYLAYRVGSFLSGGRGSGADVALS
                     QEAAGHATDVGGWVPALGNWVSSIGVPLAIGVVTLATAASLLGYLLVHAIWKWRDARR
                     RRAQRSRASGA"
BASE COUNT          492 a         1192 c         1182 g          550 t
ORIGIN      
        1 cttcgccacg ccgcgcttcc atcatctgag cgagctgtac ggctatctgg ccaagcagtt
       61 cacggtgttc ggacagcaca tccacatcgg ctgcgacagc ggcgaccgcg ccatcgggct
      121 gatccatgcg atgtcgcgct acattccgca cttcatcgcg ctgtccgccg cctcgccgtt
      181 tctgcagggg gtggacaccg ctttcgagag cgcgcgcctg aacagcgtga acgccttccc
      241 gctctcgggc catctgccgc cggtgcgcaa ctgggccgag ttcgaagcct atttcgacag
      301 catgcgcgag ctgaaggtgg tgtccagcat gaaggacttc tactgggaca tccgccccaa
      361 gcccgagtac ggcaccgtcg aggtgcgcgt atgcgacacg ccgctcacgg tggagcgggc
      421 tgcggcgctt gccgccttcg gccaggcgct cgcccgctgc ctgctggacg gcggcacccc
      481 cagggtcgat ccggaggcat accgcgtgta cggctacaac cgcttcctcg cgtgccgctt
      541 cggcctcgag gcggagctgg tcgatttcga gcggcgcgag aagctcgccc tgcgcgacga
      601 tctgctggac accctgcggc tggcgcgggg cttcgcgtcg gacacctccg gcgcggaggc
      661 tctggacaag ctggcggaag acgtgcagcg aaacgaaagc gacagcgcct ggttgcgcga
      721 gacgcaccgc cgcaagggtt ccctgagcga cgtggtgcgc gcccagtgcc aggtgtggca
      781 aagcggccag cgcgtgcgcg agccctgacg gaatgcccac acctgcccgc tgtgccggtc
      841 ggcgtctgtg cgggctcccc aggcgttcgc gggcccctgc gtgaacgact ggctgacgct
      901 ggcgctcggc gtcgcggccg ccggcgcggg cggcgagttg ttcgtgcgtg gcgccgtcgg
      961 gctggcgcgc cggatgcgta tctccccggg gatcatcggc gccaccgtgg ctgcgttcgc
     1021 cacctccagc ccggagctgt cggtcgccgt cggttccgcg ctggcgcaga ggccgcagat
     1081 ctcgctgggc gacgcgctgg gcagcaacgt cgtcaacgtc ggactgatcc tcgggctggc
     1141 actggtcatc tcaggcacca ggagcccgcg cgacgcggtc cggcgcgact tcccggcggc
     1201 gattgctgcg ccggtgctca cggccgcgtg tctgctcgac ggcgtgctgt caaggccgga
     1261 agcgatgttg ctgttcgccg gcttcattct ctggtttctc gctgcgatcc aggaagcgcg
     1321 caggcaacgc agcgccgcgc cggccgtgct cggggagcgg ctggcgggac gcgcgatcgc
     1381 agagggagcg ctcggcctga tcatgctcat cgcggccggg cgcttgatcg tgctgggcgc
     1441 gaaggggatc gcattgtcgg caggcatcgg cgagttcgtc atcggcgcga ccgtcgtggc
     1501 gatggggacc tcggtgcccg agctcgccac ggcgatcgtc tccaagctgc gcgggcacga
     1561 tgaggtcggg ctcggcacca tcctcggcag caacatcttc aacgggctgt tcgtcgtccc
     1621 gctcgcggcc gcgatcttcc cgatcgcgat tgccctgcgg gaggtggccg gcgcgctgat
     1681 cttcggcctg ctggccgttg cgctgacctt ccccgccgcc ggcggatgga tcgggcgcgg
     1741 ccgcggcgtg ctcctgctcg ccttgttcgc tgcattcctg ttctccacgc tgcgcgccgg
     1801 cctgtagccg tggatcgacc ccggggtgtt gcgaccaccc cgtgcaacgc ttgtctgaca
     1861 attcttttca cgggcagcgc ggactgctta gatttgcagg cacgcgccgg agtggaccgg
     1921 cgcgctagcc ccgacgggag gcagcgtggg catgatgcat taccggccgt tcgcgcggaa
     1981 ggccagagca atcctcaccg agcgcgacta ccgggcggcg cgagcggttc tgctggcgtc
     2041 cgccaggatg gcgttgctgc gggaggaaga agagcgagtc gaggcgctcc tgcgcgagct
     2101 ggcgcactac gactccaacc ccgcggaatt cgagccgggg ccggcgacgg acccggtgca
     2161 gtccggtccg gcccgcggct ttgacgggaa cggcccgggc cgccggtggt ccgaccgaca
     2221 ctctcgcgaa ggttagctct gccgcgatcg ctcggggtgg ggacgcgctg cctgcgcgct
     2281 ccggctcacg cgccggacgc ccgcgagcgc tgcgcccgcc gccggcgtgc gtcgcgccat
     2341 ttccagatgg cgtgcaccag gaggtaaccg agcagggaag cggctgttgc cagcgtgacc
     2401 acgccgatgg caagcggcac accgatggag cttacccagt tgcccagggc ggggacccat
     2461 ccacctacat ctgttgcatg gccggccgcc tcctgcgaga gcgcgacgtc ggcgcccgaa
     2521 ccgcgtccgc cgctgaggaa cgatcccacg cgataggcga ggtaatagac aaacggaaag
     2581 gtcaacgggt ttgtgaccag cgtgctggcg gccgccaccg ccagattggc gcgcagcagc
     2641 acggcagcga cggcggcgaa caggatttgc gccaccggca acagaatccc gaagaacaag
     2701 ccgatggcga ccccgcgtgc caccgggcgc tgctccaggc tccacaacga ccggtgccgc
     2761 agccagcgcc ggaacggccg cagcgaccaa tggttgcgca cccgccccgg cctgggaacc
     2821 catcgcgccc agcggctcct cgcctcgggc ttcgtatcac cgcgtccgcg catgacctcg
     2881 cggcgcccat cgctccgccc aacctgacgc cgccgcaggg cagcctcgct ctcgggaacg
     2941 ccgatcgcga tgtcgagtcc aagtgcataa gcctgcgtgc atctgcctgt tgacctgtac
     3001 atcgccgcct ggttccgttg ccagggccgg gcgccgtgca ccgcgctcat tgcgtcgacc
     3061 ggcggcctct gatccagcat gaacgatgcg ccacagccct tgccggctgc cggagtcatg
     3121 gtcttgccgc atcgtcctgc aatcgccgcc aacctctgga tcaagcttct cactgtggtg
     3181 ggcgcgctgg agcgcacacc ttcagcgcgc cgcccgaatc ggtggaggcg ctggtcaagg
     3241 ccgccgacgc gctcatgtac gatgccaagc gcgacggcgc gaggcggatc gagcacggct
     3301 gctcgtgacc tgtcgatcgt cggcgactgt gcctcggtgc accgtcgtgt tgcagtctcg
     3361 atcaatggag ggtgagatgg cttatagccg caccgttgcc aagaagctcc tcaacg
//
LOCUS       DASPGE010000330         4285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_322196, whole genome shotgun sequence.
ACCESSION   DASPGE010000330 DASPGE010000000
VERSION     DASPGE010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322196"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="VD791_10595"
     CDS             <1..1168
                     /locus_tag="VD791_10595"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HXV08468.1"
                     /translation="LGIGRDGKRTDRYQPGMELRARQTLFAEGCRGSLTQELFERFRL
                     HEGADPQTYGIGIKELWEIESAKSQPGLVIHTIGWPLDSATYGGSFLYHLSNNQVAVG
                     FVIGLDYRNPYLNPYEEFQRFKTHPAIRGTFEGGRRLCYGARALNEGGFQSLPRLTFP
                     GGMLVGDAAGFLNVPKIKGTHTAMKSGMTAAEAVGEHLQAGGGAEVASYPQRLARTWV
                     WGELRRVRNIRPAFRWGLHAAIAYSAIDTYLLRGKAPWTLHHHADHTQLKKASECRPI
                     AYPKPDGKLTFDRLSSVFLSNTNHEEDQPVHLTLKDALVPIGVNLALYDAPEQRYCPA
                     GVYEIVRDQHGGNPQLQINAQNCVHCKTCDIKDPTQNIRWVVPEGGGGPNYPNM"
     gene            1283..4192
                     /locus_tag="VD791_10600"
     CDS             1283..4192
                     /locus_tag="VD791_10600"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HXV08469.1"
                     /translation="MPLRRHWFHLLLLLAALLVAGTAWARPLERRSAWPHETADLAPD
                     PAVVWGRLDNGMRYVLLPNHTPRDRVSLRLLIDAGSLMEGDDQRGLAHFLEHMAFKGS
                     QNMPAGDLVQYLERLGMAFGADTNARTSFESTVYILELPANDGELIDRSLFVLREKAD
                     RLLIPAAELERERGVVLSERRLRDTAQFRAVEDNLAFLFPGTRVPERMPIGLESVIAG
                     APRERLLDFYGTYYTPSRTTLVAVGAIDPASFAQAVRKHFDSFRARGPDAPNPELGEI
                     GVRRLETRFHPEPEGRTSIVLQVAKPLPAQPDVHPRRVHEINVHLAHAIVSRRLATLS
                     MQPGAVFLGGSAHSDEFLDLARTGTVMLNARPEQWRAALAVGEQELRRALTHGFTASE
                     LDEQRRSLLSDFEERARAAATRESPDLADELADTLTEGRVFTSPAQDLAEVERIMASV
                     TTESMLQALRGLWSGSGPLVFLAGPLELDDPEAAIAQAYRTSQAVAVSAPAENAIQEF
                     AYRSFGATPQVVERRVSDVLEVTQLRFANNARANLKQTKFEANSVLVAVRFGGGRLEL
                     PRDKPGLERLAQSAFVAGGLEKHSLDELNRIIAGRSVGLGFEVEDDSFVLGGRTTPGD
                     LELQLQLLAAYLVAPGYRAEALERFRQGLPQLYQSLERTPMGVMQKDVVRFLRGGDPR
                     FGYPEQSVLAVRTLDELRAAMREALAQGYLEVSVVGDFDLERAVEAVGNTFGSLPPRA
                     TRKQSFSEARDVRFPASRGITAFAYDTNDPKALSAVYWPTTDFSKISEVRRLFVLAKV
                     LGNRVLERVRNEQGLTYTAQGDHAPSQAFPDYGFLYAIVDAPPDKARLLVSEIAALGA
                     ALHRDGVTVDELERARNPVVNELKRLLSTNSYLLSAIISGSQEQPEKLKRAATSLQEI
                     SSLTVADLNEVARKYLRPEAALPVVVVPRAPAAKKASLLPSAPLRRGLMRPQGAI"
BASE COUNT          666 a         1530 c         1479 g          610 t
ORIGIN      
        1 cctgggcatc ggtcgcgacg gcaagcgcac cgatcgctac cagccgggca tggagctgcg
       61 cgcgcgccag actctgttcg cggagggctg ccgcggctcg ctcacccagg agctgttcga
      121 gcgtttccgg ctgcacgagg gcgccgatcc gcagacctac ggcatcggca tcaaggagct
      181 gtgggaaatc gagtccgcga agtcgcagcc ggggctggtg atccatacca tcggctggcc
      241 gctggacagc gccacctacg gcggctcgtt cctgtatcac ctgtcgaaca atcaggtcgc
      301 ggtgggcttc gtgatcggcc tcgactaccg caacccgtac ttgaacccgt acgaggagtt
      361 ccagcgcttc aagacccatc ccgccatccg gggcaccttc gaaggcggcc ggcggctgtg
      421 ctacggcgcc cgggcgctga acgaaggcgg cttccagtcg cttccccgtc tcaccttccc
      481 cggcggcatg ctggtgggcg acgccgcagg ctttctcaat gtgcccaaga tcaagggcac
      541 gcacaccgcc atgaaatcgg gcatgaccgc ggccgaggcg gtgggcgagc atctgcaggc
      601 cggcggcggc gcggaggtcg cgagctatcc gcagcgcctc gcgcgcacct gggtgtgggg
      661 cgagttgcgc cgggtgcgca acatccgccc cgcctttcgc tggggtctgc acgcagccat
      721 cgcgtacagc gccatcgaca cctacctgct gcgcggcaag gcgccgtgga ccctgcacca
      781 tcacgccgac cacacgcagc tgaagaaggc gtccgagtgc cgtcccatcg cctatccgaa
      841 gcccgacggc aagctcacgt tcgaccggct gtcctcggtg ttcctgtcca acaccaacca
      901 cgaggaggac cagccggtcc acctcacgct gaaggacgcg ttggtgccga tcggtgtgaa
      961 cctggccctg tacgatgcgc ccgagcagcg ctactgcccg gcgggcgtgt acgagatcgt
     1021 gcgcgaccag cacggcggca atccgcagct gcagatcaac gcccagaact gcgtgcactg
     1081 caagacctgc gacatcaagg acccgacgca gaacatccgc tgggtagtgc ccgagggcgg
     1141 tggcgggccc aattatccga acatgtgagg cgcgaggcat atgaggcacg aggcatgagg
     1201 cgctgggcag cggtggcgaa ccgagcgcgc tcggcacggt cctagcttcg ccaacccggc
     1261 ccctctcccg gcagaatcct tcatgccgct gcgtcgacac tggttccatc tcctgctgct
     1321 gctcgcggcg cttctggtcg ccggcacggc ctgggcgcgc ccgctcgagc ggcgcagcgc
     1381 gtggccgcac gaaaccgccg acctggcgcc cgacccggcc gtggtctggg gccggctcga
     1441 caacggtatg cgttacgtgc tgctgcccaa ccacacgccc agggaccggg tcagcctgcg
     1501 cctgctgatc gacgcgggat cgctgatgga gggcgacgac cagcgcgggc tcgcgcactt
     1561 cctcgagcac atggcgttca aaggctcgca gaacatgccg gccggcgacc tggtgcaata
     1621 cctggagcgc ctgggcatgg cgttcggcgc ggacaccaat gcgcgcacca gcttcgagtc
     1681 caccgtgtac atcctggagc tgccggcgaa cgacggtgag ctcatcgacc gcagcctgtt
     1741 cgtgctgcgc gagaaggccg accgcctgct gatccccgcc gccgagctgg aacgcgagcg
     1801 cggcgtggtg ctgagcgagc ggcgcttgcg cgacaccgcg cagttccgcg ccgtcgagga
     1861 caatctcgcc ttcctgttcc ccggcacgcg ggtgcccgag cgcatgccga tcgggctcga
     1921 gagcgtgatc gccggcgcgc cgcgcgaacg gctgctggac ttctacggca cgtactacac
     1981 cccttcccgc accaccctgg tggcggtggg cgcgatcgac cccgcgagct ttgcgcaggc
     2041 ggtgcgcaag cacttcgaca gcttccgggc gcgcggcccg gacgccccca atccggagct
     2101 cggcgagatc ggcgtgcgcc ggctggagac gcgctttcac cccgagcccg aaggccgcac
     2161 cagcatcgtg ctgcaggtgg ccaagccgct gccggcgcag cccgacgtgc acccgcgccg
     2221 cgtgcacgag atcaacgtgc acctggcgca cgcgatcgtc tcgcgccgcc tcgcgacgct
     2281 gtcgatgcag ccgggcgcgg tctttctcgg cggctccgcg cacagcgacg agttcctcga
     2341 cctggcgcgc accggcacgg tgatgctcaa cgcgcgcccg gagcagtggc gcgccgcgct
     2401 ggcggtgggc gagcaggagc tgcgccgcgc gttgacacac ggcttcaccg cgagcgagct
     2461 cgacgagcag cgccgttcgc tgctgtccga cttcgaggaa cgcgcgcgcg ccgccgctac
     2521 gcgtgagtcg ccggacttgg cggacgagct ggccgacacc ctcaccgaag gccgcgtgtt
     2581 caccagcccc gcgcaggacc tggccgaggt ggagcgcatc atggcctcgg tgacgaccga
     2641 atcgatgctg caggcgctgc gcggcctctg gtcgggcagc ggtccgctgg tattcctggc
     2701 cgggccgctg gaactggacg accccgaggc ggcgatcgcg caggcgtacc ggacgagcca
     2761 ggcggtggcg gtgtccgccc ccgccgaaaa cgcgatccag gagttcgcct atcgcagctt
     2821 cggcgcgacg ccgcaggtgg tggagcggcg cgtgtccgac gtgctggagg tgacccagct
     2881 gcgcttcgcc aacaacgcgc gcgccaacct caagcagacc aaattcgagg ccaacagcgt
     2941 gctggtcgcg gtgcgcttcg gcggcggccg gctggagctg ccgcgcgaca agccgggcct
     3001 ggagcggctc gcccaatcgg cgttcgtggc cggcgggctg gagaagcaca gcctcgacga
     3061 gctcaaccgc atcatcgccg ggcgcagcgt cgggctgggc ttcgaggtcg aggacgacag
     3121 cttcgtcctg ggcgggcgca ccacccccgg ggacctcgag ctgcagctgc agctgctggc
     3181 cgcctatctg gtcgctcccg gttatcgcgc cgaggcgctg gagcgcttcc gccagggctt
     3241 gccgcagctc tatcagagcc tggagcgcac gccgatgggc gtgatgcaga aggacgtggt
     3301 gcgctttctg cgcggcgggg atccgcgctt cggctatccc gagcagagcg tgctcgccgt
     3361 gcgcaccctg gacgagctgc gcgcggcgat gcgcgaggcg ctggcgcagg gctacctgga
     3421 ggtctccgtg gtcggggact tcgacctcga gcgcgcggtg gaagcggtcg gcaacacctt
     3481 cggcagcctg ccgccgcgcg cgacgcgcaa gcagtctttc agcgaagcgc gcgacgtgcg
     3541 cttcccggcg agccgtggca tcaccgcttt cgcctacgac accaacgacc ccaaggcgct
     3601 gtcggcggtg tactggccga ccacggactt cagcaagatc agcgaggtgc gccgcctgtt
     3661 cgtgctcgcc aaggtgctgg gcaaccgcgt cctggagcgg gtgcgcaacg agcagggcct
     3721 cacctacacc gcgcagggcg accacgcgcc gagccaggcc ttccccgact acggcttcct
     3781 gtacgcgatc gtggacgccc cgcccgacaa ggcgcggctg ctggtgtcgg agatcgccgc
     3841 cctgggcgcg gccctgcacc gcgacggcgt caccgtagac gagctggagc gcgcgcgcaa
     3901 cccggtggtg aacgagctca agcgcctgct cagcaccaac tcctacctgc tctcggcgat
     3961 catcagcggc tcccaggagc agcccgagaa gctgaagcgc gcggcgacca gcctgcagga
     4021 gatctcgtcg ctcaccgtgg cggacctgaa cgaagtggcg cgcaagtacc tgcgcccgga
     4081 agcggcgctg ccggtggtgg tggtgccgcg cgcgccggcg gcgaagaaag cctccctact
     4141 gccaagtgcg cccttgaggc gcggcctgat gcgccctcaa ggcgcgattt aagaaccaac
     4201 tgccctcccc tctctcacga gggggggggg ccgctaagca ggtgcggggc cgcccgccac
     4261 agaacctcga ctcacgcccg cgcct
//
LOCUS       DASPGE010000331         5470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1491404, whole genome shotgun sequence.
ACCESSION   DASPGE010000331 DASPGE010000000
VERSION     DASPGE010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491404"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VD791_10605"
     CDS             <1..281
                     /locus_tag="VD791_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HXV08470.1"
                     /translation="IDFMHWLAGREAVPTIRALRDQAERARRHAVERALRLLHRGEPA
                     DKVLEALSQALTNKLMHAPTAALHDAAEADRLQLVRLLERVYQIRGRD"
     gene            288..1376
                     /gene="prfA"
                     /locus_tag="VD791_10610"
     CDS             288..1376
                     /gene="prfA"
                     /locus_tag="VD791_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HXV08471.1"
                     /translation="MKASIAAKLTQLSARLSEINRLLSAENATADLDAFRRLSREHAE
                     IGPVVGLYDAWRKAASDLGAAQEMTSDPEMRELAEAEIKTAKERMAGIESELQRLLLP
                     RDPNDERNIFLEIRAGTGGDESALFAGDLFRMYARYAERRRWQVEIVSQSPGEVGGYK
                     EIIAKIVGAGAYSRLKFESGGHRVQRVPVTETQGRIHTSACTVAVMPEADEIGEVVIN
                     PAEIRVDTFRSSGAGGQHVNKTDSAVRVTHLPTGIVVECQDDRSQHKNKAQALSILAA
                     RIKDKQLRERRAREAATRKSLIGSGDRSERIRTYNFPQGRVTDHRINLTLYKIDQIMD
                     GDLDDLLSALMAEHQTEQLAALAEETPA"
     gene            1451..2320
                     /gene="prmC"
                     /locus_tag="VD791_10615"
     CDS             1451..2320
                     /gene="prmC"
                     /locus_tag="VD791_10615"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HXV08472.1"
                     /translation="MTLSATCDALLVRDARRIAAALALPEREARLEAELLLAHALGVT
                     RAQLIARPERAADALHSSGYPEYLERRLGGEPLAYIIGEREFYGLPFRVTPAVLIPRP
                     ETELLVEMALQRIEPAASARVLDLGTGSGCIAVSIARLRPYAQVVATDVSPAALTVAE
                     ANALRHRAANVKFRLGDCYAPVAEAQFDLIVANPPYVAEADPHLNRGDVRYEPRHALV
                     SGADGLMLSRRLVARAPGHLRAGGWLLVEHGHDQAGPVRELFEATGFGDFCCARDLAG
                     LPRAAAGRLTLKP"
     gene            2389..2694
                     /gene="grxD"
                     /locus_tag="VD791_10620"
     CDS             2389..2694
                     /gene="grxD"
                     /locus_tag="VD791_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015420309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HXV08473.1"
                     /translation="MDVQDKIRKQVTDNRIVLYMKGTPEAPQCGFSAAAVQILEACGA
                     EEVATVNVLADADIRQGIKEFSNWPTIPQLYVNGEFVGGTDIMREMYQSGELQKLVK"
     gene            2762..3922
                     /locus_tag="VD791_10625"
     CDS             2762..3922
                     /locus_tag="VD791_10625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010115858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXV08474.1"
                     /translation="MQSASDDFPELRQAVRALCATFAPEYWRELDERRAYPEAFVKAL
                     TDAGWLAALIPEEYGGAGLGLTEASVILEEVNRSGANSGACHAQMYIMGTLLRHGSAE
                     QKARYLPQIAAGKLRLQSFAVTEPTTGSDTTRLRTTAVRKGDRYVVNGQKVWTSRVQH
                     SDLLLLLARTTPLPEVKKRSEGLSVFLVDLRETAGKGITLRPIRNMPNHETNEVFFDH
                     LEVPSANLIGEEGKGFRYILDGMNAERILIAAECVGDGHWFLEKAAGYARERVVFDRP
                     IGQNQAVQFPIARAYVNVEAADLMRFKAAALFDRGEPCGAQANMAKLLAADASWEAAN
                     ACLQTHGGFGFAAEYDVERKFRETRLYQVAPISTNLILSYIAEHVLGLPRSY"
     gene            3991..4632
                     /locus_tag="VD791_10630"
     CDS             3991..4632
                     /locus_tag="VD791_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXV08475.1"
                     /translation="MHALACSLVAWLLCLAAALAGASARAEGTPDPAHALAGSALAAA
                     LAQGGFVIYFRHADTGPAVPEPPGFDLARCGTQRNLNDDGRAQARDIGAQFRRLDIPV
                     GRVLSSEFCRCWQTAELAFGRYEKTSDLTGVPRGAQWDDLRAQRSAALRELLSSPPPP
                     GQNSILVSHGFNLWDLERFHLGTQGEAAVYRPDGRGAYALVARLLPQDWAELR"
     gene            4686..4997
                     /locus_tag="VD791_10635"
     CDS             4686..4997
                     /locus_tag="VD791_10635"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08476.1"
                     /translation="MRSHHDMGGEPAGPVQRSEHDYAFWEKRVDALLVLLTRKQRMNV
                     DELRRNVESLGAEAYDKLSYYERWILSITQTLIQRGVISVDELGRKMAEIERRPRDPG
                     P"
     gene            4994..5302
                     /locus_tag="VD791_10640"
     CDS             4994..5302
                     /locus_tag="VD791_10640"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HXV08477.1"
                     /translation="MSTPRIKPIAQRFQAGDSVRVRRADPPGHVRTPWYIRGQTGVVE
                     RVCGSYPNPEELAYARPGLPAQPLYRVRFLQRQVWPDYTGGARDTVDIEIYQHWLDPA
                     "
BASE COUNT          912 a         1877 c         1884 g          797 t
ORIGIN      
        1 tcatcgactt catgcactgg ctggccggtc gcgaggcggt tccgaccatc cgcgcgctgc
       61 gcgaccaggc cgagcgcgcc cgccgccacg cggtcgagcg cgcgctgcgc ctgctgcacc
      121 gcggcgagcc ggcggacaag gtgctcgagg cgctgagtca ggcgctgacc aacaagctga
      181 tgcacgcacc gaccgcggcg ttgcacgatg ccgccgaggc cgaccggctg caactcgtgc
      241 gtctgctcga gcgcgtgtac cagatccgcg ggcgcgactg aaagcgaatg aaggcgagca
      301 tcgccgccaa gctgacgcag ctgtcggccc gtctgtcgga aatcaaccgc ctgctgtcgg
      361 cggagaacgc caccgccgat ctggacgcgt tccgcaggct gtcgcgcgag cacgccgaga
      421 tcggcccggt ggtcgggctc tacgacgcgt ggcgcaaggc cgcgtccgac ctgggcgccg
      481 cccaggagat gacctccgac ccggagatgc gcgagctggc ggaagccgag atcaaaaccg
      541 ccaaggagcg catggccggc atcgagagcg agctgcagcg cctgctgctg ccacgcgacc
      601 ccaacgacga gcgcaacatc ttcctggaga tccgcgccgg taccgggggc gacgagtcgg
      661 cactgttcgc cggcgacctg ttccgcatgt acgcgcgcta cgccgagcgc cggcgctggc
      721 aggtggagat cgtgtcgcag agccccggcg aggtcggcgg ctacaaggag atcatcgcca
      781 agatcgtcgg cgcgggcgcc tactcgcgcc tgaagttcga gtccggcggc caccgcgtgc
      841 agcgcgtgcc ggtcaccgag acgcaaggcc gcatccacac cagcgcctgc acggtggcgg
      901 tgatgccgga ggcggacgag atcggcgaag tggtgatcaa cccggccgag atccgcgtgg
      961 acacgtttcg ctcgtccggc gcgggcgggc agcacgtcaa caagaccgac tcggcggtgc
     1021 gcgtgacgca cctgcccacc ggcatcgtgg tggagtgcca agacgaccgc tcgcagcaca
     1081 agaacaaggc gcaggcgctg tcgatcctgg ccgcgcgcat caaggacaag cagctgcgcg
     1141 agcgccgcgc cagggaggcc gccacccgca agtccctgat cggcagcggc gaccgttcgg
     1201 agcgcatccg cacctacaac ttcccgcagg gccgcgtcac cgaccatcgc atcaacctga
     1261 cgctgtacaa gatcgaccag atcatggacg gcgacctgga cgatctgctg agcgcgctga
     1321 tggccgagca ccagaccgag cagctcgctg cgctggccga ggagacgccg gcgtaagcgc
     1381 gttgcaggct caccgaagct tgcggatgga cgcgaccgcc cgcgtgaatg cgccccttgc
     1441 agcgcagccg atgaccctct cagcgacctg cgacgccttg ctcgtgcgcg atgctcggcg
     1501 catcgccgcg gcgcttgcgc tgcctgagcg cgaggcgcga ctggaggcgg agctgctgct
     1561 cgcgcacgcc ctcggcgtca cgcgcgcaca gctgatcgct cgtcccgaac gagccgcgga
     1621 tgcgctgcac agcagcgggt atccggaata tctcgagcgc cgcctgggcg gcgagccgct
     1681 cgcctatatc atcggcgaac gcgagttcta cggcttgcca ttccgggtca cgcccgcggt
     1741 gctgattccg cgtcccgaga cggagctgct ggtggagatg gcgctgcagc gcatcgagcc
     1801 ggccgcctcg gcgcgggtgc tggacctggg cacgggcagc ggttgcatcg cggtgagcat
     1861 cgcccgtctg cggccgtacg cgcaagtcgt ggccacggac gtttcgccag cggcattgac
     1921 cgtggccgag gcgaacgcgc tgcggcaccg ggcggcaaac gtgaaatttc gcctgggaga
     1981 ttgctatgcg ccggtggcgg aggcgcagtt cgacctgatc gtcgccaacc cgccgtacgt
     2041 cgcagaagcc gacccgcact tgaaccgggg ggacgtgcgc tacgaaccgc ggcacgcgct
     2101 cgtgtcgggt gcggacgggc tcatgctgtc gcgacggttg gtggcgcgcg cgcccggtca
     2161 tctgcgtgcg ggcggttggc tgctggtaga gcatggccac gatcaagccg ggccggtgcg
     2221 cgagctgttc gaggccacgg ggttcgggga cttttgctgc gcccgcgacc tcgctggcct
     2281 gccccgtgca gcggccggcc ggttgacgct aaagccttga aaccgataac atcgtgccca
     2341 tgtcacaggg atcccgagcg gggttcctca gatccttcga ggtgagccat ggacgtccag
     2401 gacaagattc gcaagcaggt gaccgacaac cgcatcgtgc tgtatatgaa gggaacgccg
     2461 gaggcgccgc agtgcggatt ctcggccgcc gcggtgcaga tcctcgaggc ctgcggcgcc
     2521 gaggaggtgg ccacggtcaa cgtcctggcc gatgccgaca tccgccaggg catcaaggag
     2581 ttctccaact ggccgacgat cccgcagctg tacgtgaacg gcgaattcgt cggcggcacg
     2641 gacatcatgc gcgagatgta ccagagcggc gagctgcaga agctcgtcaa gtagcgccgt
     2701 tgccggatca gaagggctgg ctctgccagc ccttcgtctt ttcaaacggg atcgaccgcc
     2761 cttgcagagc gcatcggacg actttcctga gttgcggcag gcggtgcgcg ccctgtgcgc
     2821 cacctttgcg cccgagtact ggcgtgagct cgacgagcgg cgcgcctatc ccgaggcctt
     2881 cgtcaaggcg ctcaccgacg cgggctggct ggccgcgctc attccggagg aatacggcgg
     2941 cgcgggcctc ggtctgaccg aggcctcggt gatcctggag gaggtgaacc gctccggcgc
     3001 caactccggc gcctgtcatg cccagatgta catcatgggc acgctgctgc gccacgggtc
     3061 ggccgagcag aaggcgcgct atctcccgca gatcgccgcc ggcaagctgc ggctgcagtc
     3121 tttcgcggtg accgagccga ccaccggttc cgacaccacc aggctgagga ccaccgccgt
     3181 gcgcaagggc gatcgctacg tggtgaacgg gcagaaggtg tggacctcgc gcgtgcagca
     3241 ttcggatctg ctgttgctgc tcgcgcgcac cacgccgctg cccgaggtga agaagagatc
     3301 cgaaggcctg tcggtgttcc tggtggatct gcgcgagacg gcgggcaagg ggatcaccct
     3361 gcgcccgatc cgcaacatgc ccaaccacga gaccaacgag gtgttcttcg accatctcga
     3421 ggtgccgtcc gcgaacttga tcggcgagga gggcaagggc ttccgctaca tcctcgacgg
     3481 catgaacgcc gagcgcatcc tgatcgccgc cgagtgcgtg ggcgacggcc actggttcct
     3541 ggagaaggcc gccggctatg cccgcgagcg ggtcgtgttc gaccgcccga tcggccagaa
     3601 ccaggcggtg cagttcccca tcgcgcgcgc ttacgtgaac gtcgaggccg cggacctgat
     3661 gcgcttcaag gcggctgcgc tgttcgaccg cggcgagccc tgcggcgcgc aagccaacat
     3721 ggccaagctg ctcgccgccg acgcttcctg ggaagcggcc aacgcctgcc tgcagaccca
     3781 cggcggcttc ggtttcgccg cggagtacga tgtcgagcgc aagttccgtg agacgcgcct
     3841 gtaccaggtg gcgccgatct cgaccaacct gatcctgtcc tacatcgccg agcacgtgct
     3901 cggcctgccg cggtcctact gatcgccgcc ccgatctgcg cgaaaatggc aagcatggaa
     3961 cgccttggcc gtcccgcctc agagagaagc atgcacgcct tggcctgcag tttggtcgcc
     4021 tggctgctgt gtcttgccgc cgcgctcgcc ggcgccagcg cgcgcgcaga gggaacaccc
     4081 gacccggcgc acgccctcgc cggctccgcg ctggctgcgg cactcgcgca aggcggcttc
     4141 gtgatctact tccgccacgc cgacaccggc cccgcggtgc ccgagccgcc cggtttcgac
     4201 ctcgcgcgct gcggaacgca gcgcaacctc aacgacgacg gccgcgcgca ggcgcgcgac
     4261 atcggtgcgc agttccgccg cctcgacatc cccgtgggcc gtgtattgtc gagcgagttc
     4321 tgccgctgct ggcagaccgc cgagctggcg ttcggccgct acgagaaaac cagcgacttg
     4381 accggcgtgc cgcgtggagc gcagtgggac gatctgcgcg cgcagcgctc cgcggcgttg
     4441 cgcgagctgc tgtcgtcgcc gccgccgccg ggacagaaca gcattcttgt gtcgcacggc
     4501 ttcaacctct gggacctgga acgcttccac ctcggcaccc agggcgaggc agccgtgtac
     4561 cgccccgacg gccgtggtgc ttacgccctg gtcgcgcgtc tgctgccgca ggattgggca
     4621 gagctcagat gagtggaggc cagcgcagat gacgcaacat cggcgcagcg ccaccgcagg
     4681 caaacatgcg tagccaccac gacatgggcg gcgagccggc cggtccggtg cagcgcagtg
     4741 agcacgacta tgcgttctgg gagaagcgcg tcgacgcgct gctggtgctc ctgacgcgca
     4801 agcagcggat gaacgtggac gagctgcggc ggaacgtcga gagcctgggc gcggaagcct
     4861 acgacaagct ttcctactac gagcgctgga ttctttccat cacgcagacg ctgatccagc
     4921 gcggcgtcat cagcgtggac gagctcgggc gcaagatggc ggagatcgaa cggcgccctc
     4981 gagaccctgg cccgtgagca cgccgcgaat caaaccgatc gcacagcggt ttcaagcggg
     5041 cgacagcgtg cgcgtgcggc gagcggatcc gccgggtcac gtgcgcacgc cctggtacat
     5101 ccgcgggcaa acgggcgtgg tcgaacgcgt ctgcggttct tatcccaacc cggaagaact
     5161 cgcctatgcg cgcccggggc tgcccgcgca gcccctgtac cgagtgcggt tcctgcagcg
     5221 ccaggtgtgg ccggactaca ccggcggcgc gcgggacacc gtggacatcg agatctacca
     5281 acactggctg gaccccgctt gacgaggcgc acatgcacgc tcattccgac cacgaccatc
     5341 cacacccgcc cgacagcgac gacgcgctga cctactacca ggcgatggag atcgcggtgc
     5401 gcgagctgct gatcgagaag ggcgtgctca ccgccgacga gctgcggcgc caggtcgagg
     5461 acatggacaa
//
LOCUS       DASPGE010000332         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_322493, whole genome shotgun sequence.
ACCESSION   DASPGE010000332 DASPGE010000000
VERSION     DASPGE010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322493"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VD791_10645"
     CDS             <1..304
                     /locus_tag="VD791_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HXV08478.1"
                     /translation="GAGGAPGGPGGGPPSGRGAPSGRGGPSGRGAPSGPVEPVNLVNV
                     RYQHADYEEALAEGAHAEAEAPKAQPFVRQGQKVGRNDPCPCGSGKKYKQCHGRLA"
     gene            351..1577
                     /gene="argJ"
                     /locus_tag="VD791_10650"
     CDS             351..1577
                     /gene="argJ"
                     /locus_tag="VD791_10650"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HXV08479.1"
                     /translation="MAVALTPPAREALLPVPGVRLGIACAGIRKADRQDLLLMLLAEG
                     AATAGLFTRNRFCAAPVLVCREHLAAGASVRAIAVNTGNANAGTGERGLVDARAVCAE
                     VARLTGVGAQQVLPFSTGVIMEPLPVDRILRGLPQCVADLREDNWLGAAEAIMTTDTV
                     PKAASRRLRVGGREVTLTGIAKGAGMIHPDMATMLSFVATDAAVAPGLLRGMLAHACE
                     QSFNAITVDGDTSTNDSFILAATGASGVRVEREGSVEYGALRDALTDLAIQLAQAIVR
                     DGEGATKFIALRVEQGRSREECRKVAFAIAHSPLVKTAFFAGDPNLGRILAAIGNAGI
                     DDLEVDGIELYLDEVRVASRGGRDPAYREQDGKRVMQRDEICVRVVLGRGSAAATVWT
                     CDLSHDYVTINAEYRT"
     gene            1675..2553
                     /locus_tag="VD791_10655"
     CDS             1675..2553
                     /locus_tag="VD791_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV08480.1"
                     /translation="MTELTEVLERADALLQRIERLLPPEPPAPDFAATSAWRWRSRGE
                     RGWLQPVLHPHGIRLEDLQDIDRQRDLVHRNTLQFVRGLPANNVLLTGARGTGKSSLV
                     KALLNRYRGDGLRLIELDKHDLMHLPDIVERLAAQAYRFILFCDDLSFDADEPGYIPL
                     KAVLDGSVSATPENVLIYATSNRRHLMPEYMQENLETRYVGGEVHPGESVEEKISLSE
                     RFGLWLSFYPFNQDEYLDIVRHWLRELQVADADGEEVRREALQWALQRGSRSGRVAWH
                     FARDWAGRHGLSAVRR"
     gene            2559..>2931
                     /locus_tag="VD791_10660"
     CDS             2559..>2931
                     /locus_tag="VD791_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXV08481.1"
                     /translation="MDSAELVQVAAAVVLREDGRFLLAQRPAGKAYAGYWEFPGGKAE
                     PGEPLADALARELHEELGIDVTRAYPWIVRRYRYPHAHVQLNFFRVLGWSGEPHPRED
                     QALAWAALHDLSVTPLLPANGP"
BASE COUNT          440 a         1023 c         1068 g          400 t
ORIGIN      
        1 cggcgcgggc ggtgcgccgg gcggcccagg cggtggcccg ccgagcggcc gcggcgcacc
       61 gagcggccgc ggcggaccga gcggccgcgg cgcaccgagc ggtccggtgg agcccgtcaa
      121 tctggtgaac gtgcgctatc agcacgccga ctacgaggag gcgctggcgg agggtgcgca
      181 cgcggaagcc gaggcgccca aggcccagcc cttcgtgcgg cagggccaga aggtggggcg
      241 caacgacccc tgcccctgcg gttccggcaa gaaatacaag cagtgccatg ggcggctggc
      301 gtgacgccgg ccgccggcgc tgagcccgcc ggtcggacat ccgcctggcc gtggccgtcg
      361 ccctgacccc gcccgcgcgc gaagcgctcc ttccggtgcc gggggtgagg ctgggaatcg
      421 cgtgcgccgg catccgcaag gcggatcgcc aggacctgct gctcatgctg ctcgccgagg
      481 gcgctgccac agcaggcctg ttcacccgca accgcttctg cgcggcgccg gtgctggtct
      541 gccgcgagca cctggctgcg ggagcgtccg tgcgggcgat cgcggtgaac accggcaacg
      601 ccaacgcagg caccggggag cgcggactgg tcgacgcgcg cgccgtctgc gcggaggtcg
      661 cgcgcctgac cggggttggc gcgcagcagg tgctgccgtt ctccaccggc gtgatcatgg
      721 agccgctgcc cgtggaccgc atcctgcgcg gcctgccgca gtgcgtggcc gatctgcgcg
      781 aggacaactg gcttggcgcc gccgaagcga tcatgaccac ggacacggtg ccgaaagcgg
      841 cctcgcgcag gttgcgcgtc ggcgggcgcg aggtcacgct caccggcatc gccaagggtg
      901 ccggcatgat ccatcccgac atggcgacca tgctgagctt cgtcgccacc gacgccgcgg
      961 tggcgccggg gctgctgcgg ggcatgctgg cgcacgcctg cgagcaatcg ttcaacgcca
     1021 tcaccgtgga cggcgacact tccaccaacg attccttcat tctggccgcg accggcgcca
     1081 gcggggtgcg cgtcgagcgc gagggcagcg tcgagtacgg ggccctgcgc gacgcgctca
     1141 ccgatctcgc catccagctc gcccaggcca tcgtgcgcga cggggaaggg gcgaccaagt
     1201 tcatcgcatt gcgcgtcgag cagggccgca gtcgcgagga gtgtcgcaag gtagcgttcg
     1261 ccatcgcgca ctcgccgctg gtcaagaccg ccttcttcgc cggcgatccg aatctcgggc
     1321 gcatcctggc ggccatcggc aacgcgggca tcgacgatct cgaggtggac ggcatcgagc
     1381 tgtatctgga cgaggtgcgg gtggcgagcc gcggcggacg cgaccctgcc tatcgcgaac
     1441 aggacggcaa acgcgtgatg cagcgcgacg agatctgcgt cagggtggtg ctgggacgcg
     1501 gcagcgccgc ggccacggtg tggacctgcg acctgtcgca tgattacgtc acgatcaatg
     1561 ccgagtatcg gacgtgaaaa gcgtgaccga acggcgtgac cgagaatcgt gaccgagagg
     1621 cgtgacgacc gccgcgtagc ccgtatgcag cgcagcgcga ctcagcgaag caaagtgacg
     1681 gaactgaccg aggtcctgga acgggccgac gcgctcctgc agcgcatcga gcgcctgctg
     1741 ccgcccgagc cgccggcgcc cgacttcgcc gcgaccagcg cctggcgctg gcgcagccgc
     1801 ggcgagcgcg gctggctgca gccggtgctg caccctcacg gcatccgcct ggaggatctg
     1861 caggacatcg accggcagcg tgacctcgtg caccggaaca cgctgcagtt cgtgcgcgga
     1921 ctgcccgcca acaacgtgct gctcaccggc gcgcgcggca cgggcaagtc ctcgctggtc
     1981 aaggccctgc tcaaccgcta ccggggcgac ggcctgcgcc tgatcgagct ggacaagcac
     2041 gatctgatgc acctgcccga catcgtcgag cggctggcgg cgcaagccta ccgcttcatc
     2101 ctgttctgcg acgacctgtc cttcgacgcc gacgagccgg ggtacattcc cctcaaggcg
     2161 gtgctcgacg gttcggtgtc cgccacgccc gagaacgtgc tgatctacgc cacctcgaac
     2221 cggcgccacc tgatgcccga gtacatgcag gagaacctcg agacccgcta cgtcggcggc
     2281 gaagtgcatc cgggcgagag cgtggaggag aagatctcgc tgtccgagcg cttcggcctg
     2341 tggctgtcgt tctacccctt caaccaggac gagtacctgg acatcgtgcg ccactggctg
     2401 cgcgagctgc aggtggcgga cgccgacggc gaggaagtgc gccgcgaggc cctgcagtgg
     2461 gcgttgcagc gcggctcgcg cagcggccgc gtcgcctggc atttcgcgcg cgactgggcg
     2521 ggccgccacg gcctgtcggc ggtgcgccgc tgactgcgtt ggacagcgcg gagctggtgc
     2581 aggtcgccgc cgcggtcgtc ctgcgcgagg acggccgctt cctgctggcg cagcgtcctg
     2641 ccggcaaggc gtatgcgggc tactgggagt tcccgggcgg gaaagcggag cccggcgagc
     2701 cgctcgccga tgccttggca cgcgagctgc acgaggagct gggcatcgac gtcacgcgcg
     2761 cctatccgtg gatcgtgcgg cgctaccgct acccgcacgc gcacgtgcag ctcaatttct
     2821 ttcgcgtcct gggctggagc ggggagcccc accccaggga agaccaggcg ctggcatggg
     2881 ccgcgctgca cgacctgagc gtgacgccgc tgctgcccgc caacggaccg g
//
LOCUS       DASPGE010000333         9782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1609764, whole genome shotgun sequence.
ACCESSION   DASPGE010000333 DASPGE010000000
VERSION     DASPGE010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9782
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609764"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VD791_10665"
     CDS             complement(<1..185)
                     /locus_tag="VD791_10665"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901973.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HXV08482.1"
                     /translation="MAEIRNYTMNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLH
                     RATEKLAEHKTYLQALP"
     gene            complement(334..942)
                     /locus_tag="VD791_10670"
     CDS             complement(334..942)
                     /locus_tag="VD791_10670"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HXV08483.1"
                     /translation="MPGRIEDLRTALAAQLGARIVSETEALGELTIVVKADDLLEACA
                     ALRDAPGLRFDLLVDLCGLDYSQYADGAWDGPRFAVAYHLLSLELNQRLRVRVFARDD
                     ALPVLPSATSVWPAANWYEREAFDLLGIVFTDHPDLRRILTDYGFVGHPFRKDFPLSG
                     TVEMRYDPDQQRVIYQPVTIEPREIVPRVIREEHYGDVEEKR"
     gene            complement(975..1454)
                     /locus_tag="VD791_10675"
     CDS             complement(975..1454)
                     /locus_tag="VD791_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HXV08484.1"
                     /translation="MSIEGILERGFVTTKLDDVINWTRTGSLWPMTFGLACCAVEMMH
                     AGAARYDLDRFGIMFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVVSMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGLIQLQNKIKRTGTIARM"
     gene            complement(1475..1831)
                     /locus_tag="VD791_10680"
     CDS             complement(1475..1831)
                     /locus_tag="VD791_10680"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HXV08485.1"
                     /translation="MLQQYFPILLFILVGVAVGVVPVALGWLIGPNKPDSEKLSPYEC
                     GFEAFEDARMRFDVRYYLVAILFILFDLEIAFLFPWAVVLEEIGTFGFVSMMVFVAIL
                     VVGFIYEWKKGALDWE"
     gene            complement(1979..2063)
                     /locus_tag="VD791_10685"
     tRNA            complement(1979..2063)
                     /locus_tag="VD791_10685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2027..2029),aa:Leu,seq:gag)
     gene            complement(2155..2547)
                     /gene="secG"
                     /locus_tag="VD791_10690"
     CDS             complement(2155..2547)
                     /gene="secG"
                     /locus_tag="VD791_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HXV08486.1"
                     /translation="MMQVLILLLHVAAAIAVCGLVLLQHGKGADMGAAFGGGASGSLF
                     GASGSANFLSRSTAVLATVFFLTSLGLTWYAARKPETGSVMDAHKVEQPATPAAPAGD
                     VPQPAAPPDAEPPDSTPGGDSKAQEIPK"
     gene            complement(2544..3314)
                     /gene="tpiA"
                     /locus_tag="VD791_10695"
     CDS             complement(2544..3314)
                     /gene="tpiA"
                     /locus_tag="VD791_10695"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HXV08487.1"
                     /translation="MRAKLVAGNWKMHGSLPENAALLGGVRHGSIALRRSRIAVCVPF
                     PYLFQARQALAGSAVAWGAQNLSQHPKGAYTGEVSAAMVKEFGSTYAIVGHSERRVAY
                     SEDDATVAAKFGAALATGLVPILCVGETLAQREAGETEPVVAAQLAAAIGRHGVQALG
                     RAVLAYEPVWAIGTGRTATPEQAQAVHAFLRARIAEQDREVADRSTILYGGSVKGANA
                     GELFAMPDIDGGLVGGASLVLDEFMAICRAADRTDSEA"
     gene            complement(3375..4151)
                     /gene="pstB"
                     /locus_tag="VD791_10700"
     CDS             complement(3375..4151)
                     /gene="pstB"
                     /locus_tag="VD791_10700"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HXV08488.1"
                     /translation="MASGAMTIKAETKALNFHYGQYHALKSISMPLYERKVTALIGPS
                     GCGKSTYLRCFNRMHDLYPGNRYQGEIKLYPDDTNLLHPQVDPIEVRMRISMVFQKPN
                     PFPKSIYENVAYGLRVRGVRKVAAIDEKVEQALRSAALWDEVKDRLKQLAFNLSGGQQ
                     QRLCIARALATDPEILLFDEPTSALDPIATASIEELIADLKSKLTILIVTHNMQQAAR
                     VSDYTAYMYLGELIEFDATDTIFIKPKNKATEDYITGRFG"
     gene            complement(4199..5122)
                     /gene="pstA"
                     /locus_tag="VD791_10705"
     CDS             complement(4199..5122)
                     /gene="pstA"
                     /locus_tag="VD791_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HXV08489.1"
                     /translation="MDHASIREIRELIARHKRWDLMFAAVGLLCLAVAAVTFTALVVD
                     MALDGAPRLSWDFFTNFPSRRPAQAGILSAWIGSTLVMVVTAAAAIPVGVASAVYLEE
                     YAPKNWFTAIIEINITNLAGVPSIVYGLLALGLFVYAFGLGQSILAAGLTLGLLILPV
                     VIVATREAIRSIPSMIREAAYAVGATKWQTTLDHVLPYAMPGIMTGVIIGMARAIGET
                     APIITIGALTFIAFLPPAPVTAEFPFLSFEWLMAPFTVMPIQMFNWTSRPEAAFHQNA
                     AAAGLVLLLMTLSMNALAIYIRYQLRKRIKW"
     gene            complement(5137..6096)
                     /gene="pstC"
                     /locus_tag="VD791_10710"
     CDS             complement(5137..6096)
                     /gene="pstC"
                     /locus_tag="VD791_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HXV08490.1"
                     /translation="MPPPSASYTEGLIAVADYVVSDRLSRKRTRHLRERLIEFILFGA
                     AAFSVAVTLGIVYVLVSESVLFFQHVSIIDFLTDTQWTPLFDDAHYGIMVLLSGTLVS
                     SAVALAVAVPLGTIIAIYLSEFAPFSVREAAKPLLELLSGVPTVVYGYFALLFVTPLL
                     QKLIPGLPGFNLLSAGLVMGIMIIPYVSSVSEDAMRAVPMSMREGSYAMGATRYQTAI
                     RVVTPAALSGITAAYILGISRAVGETMILAVAAGMQPNLTWNPMEPAATITSFIVQVA
                     LGDLPHGSIGYQTIFAAGLTLMLMTLLFNILGHVMTRRFRQVY"
     gene            complement(6213..7220)
                     /locus_tag="VD791_10715"
     CDS             complement(6213..7220)
                     /locus_tag="VD791_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HXV08491.1"
                     /translation="MKLHCLSRIALAAGAAATIGALSLPAQAQLVKIDGSSTVFPITE
                     AMAEDFQKSTKGKVRVTVGISGTGGGFKKFCRGETDISDASRPILKSEMEACKAAGIQ
                     YYELPVAYDALTVVINPKNTWIKSLTPAELKKMWEPSAQGKVKSWKQVNAAWPDQPLK
                     LFGPGADSGTFDYFTEAINGKSKASRGDFTASEDDNVLVQGVSRDVNALGFFGFAYYI
                     ENQDKLMAVPIAPEPGKTAVGPSMDAVINGSYTPLSRPIFIYVNAKSLERPEVQQFVE
                     FYMKNADVLVREVKYVPLPAKAYTYNMEHLAKKQVGTKFGGEAKVGLTIEDLMKMEAK
                     L"
     gene            complement(7357..8718)
                     /gene="glmM"
                     /locus_tag="VD791_10720"
     CDS             complement(7357..8718)
                     /gene="glmM"
                     /locus_tag="VD791_10720"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HXV08492.1"
                     /translation="MSRKYFGTDGVRGKVGELPITPELVMHLGYAAGKVLAGADRRPS
                     GSEPPAVLIGKDTRISGYMLESALQAGLSAAGVDIYLCGPMPTPAVAYLTRALRLSAG
                     IVISASHNPFEDNGIKFFSAAGDKLPDATEQEIETRLSDPIVTAAPAQLGKARRINDA
                     AGRYIEFCKSTFPTDLDLRGLRIVVDCAHGASYHIAPHVFHELGADVMAIGNQPDGLN
                     INLDCGATRPAALIAAVAEHGADLGLALDGDGDRLLMVDGSGCTYDGDQLLYVIARRR
                     LEDGGLRGGVVGTLMSNFGLEQALAALAVPFARAPVGDRYVLEELRARGWQLGGENSG
                     HIICLDKHTTGDGIVSALQVLHAMRAKRQPLAELLGGLRLYPQVLVNVRVERSADFRK
                     NPLVQDAVRAAEVALEGRGRVLLRPSGTEPVIRVMVEGEAAEEVQGLAQSIAEAIRQQ
                     AAA"
     gene            complement(8852..9712)
                     /gene="folP"
                     /locus_tag="VD791_10725"
     CDS             complement(8852..9712)
                     /gene="folP"
                     /locus_tag="VD791_10725"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HXV08493.1"
                     /translation="MQGARAPAASPRLRCGRFLLPLTRPLIMGVVNITPDSFSDGGRF
                     LDPAAAAEHASRLAEEGADILDLGAESTRPGAAPVSAEAEAARVLPVLDAVRGLPLPI
                     SVDTRKPETMRAALSHGASMINDIDALQAPGALDAVRDSDCGLCLMHKQGDPQTMQVA
                     PAYTDVMREVKEFLAQRVAACSAAGIDRERLAVDPGFGFGKTAAHNLELLRRLGELAQ
                     LGMPVLAGLSRKTTLGEITGRAPGDRLAASVAAALLAVQRGAAIVRVHDVAATRDALL
                     VLSALQEAER"
BASE COUNT         1619 a         3325 c         3231 g         1607 t
ORIGIN      
        1 taaggcaggg cctgcaggta ggtcttgtgc tccgccagct tctcggtagc gcgatgcagc
       61 aggccgatgt gcggatcggc gcgctggatc acctcgccat cgagctccag caccagccgc
      121 aacaccccgt gcgccgcggg atgttgaggt ccgaagttca tcgtgtagtt gcgaatttca
      181 gccatctttg tctactgcaa gagctttacc gcaaaggcgc aaaggccgcg caaaggaaac
      241 cgggagcaca ccgctggtcc aaaggcggca tgcttcactt tgcggcacct ttgcgccttt
      301 gcgccttcgc ggtcctcgct ttgctcttcg cgttcatcgt ttttcttcga cgtcgccgta
      361 gtgctcctcg cggatcaccc gtggcacgat ctcgcgcggc tcgatcgtca ccggctgata
      421 gatcacgcgc tgctgatcgg ggtcgtagcg catctccacc gttcccgaca gcgggaagtc
      481 cttgcggaac gggtggccga cgaacccgta gtcggtgagg atgcggcgca ggtcgggatg
      541 atcggtgaac acgatgccca gcaggtcgaa agcttcgcgc tcgtaccagt tggcggcggg
      601 ccagaccgaa gtcgccgaag gcagcaccgg cagagcgtcg tcgcgggcga atacgcgcac
      661 gcgcagccgc tgattcagct ccagcgacag caggtggtac gcgacggcga agcgcggacc
      721 gtcccaggcg ccatccgcat actggctgta gtccaacccg caaagatcca ccagcaggtc
      781 gaagcgcaaa ccgggcgcgt cgcgcagcgc cgcgcacgcc tcgagcagat cgtcggcttt
      841 gacgacgatc gtcagctcgc ccagcgcctc ggtctccgag acgatccgcg cgccgagctg
      901 ggccgcgagc gcggtgcgaa ggtcctcgat cctgcctggc atctggtttt gcttcgggtt
      961 cttgaaaccg cccctcacat gcgggcaatc gtccccgtgc gcttgatctt gttctgcagc
     1021 tgaatcaatc cgtacagcag cgcctccgcg gtgggcggac agcccgggac gtacacgtcc
     1081 accggcacga tacggtcgca gccgcgcacc acggagtagg agtagtggta gtagccgccg
     1141 ccgttggcgc acgagcccat ggagaccacc cagcgcggct cggccatctg gtcgtacacc
     1201 ttgcgcagcg cgggcgccat cttgttgcac agggtgccgg ccacgatcat cacgtccgac
     1261 tgccgggggc tgggccggaa catgatgccg aagcggtcga ggtcatagcg cgccgcgccg
     1321 gcgtgcatca tctccactgc gcagcaggcc aggccgaagg tcatcggcca cagcgacccg
     1381 gtgcgggtcc agttgatcac gtcgtcgagc ttggtggtga cgaagccgcg ctcgagaatg
     1441 ccttcgatgc tcataggggc aaagtctcta caggtcactc ccaatcgaga gcgcccttct
     1501 tccattcgta gatgaagccg accacgagaa ttgcaacgaa caccatcatg gagacgaagc
     1561 cgaaggtacc gatctcctcc agcaccacgg cccaggggaa gaggaaggcg atctcgagat
     1621 cgaacaggat gaacaggatc gccaccaggt aatagcgcac gtcgaagcgc atgcgcgcgt
     1681 cctcgaacgc ctcgaaaccg cactcgtagg gggacagctt ctcgctgtcc ggcttgttgg
     1741 gacctatcag ccagcccagc gcgaccggca cgacgccgac ggccactccg accagaatga
     1801 acagcaggat cgggaagtac tgttgcagca tctcgtgtcg aggcccgacc cctcaagggc
     1861 aggcggctct cctgtcaacg ggccccggcg cagaagccct caacgcgccg cgggcgcgtc
     1921 cgtaacttga gcccccctcc gcgggcaggc cgggtcggct gcctgccctt aagcgttctg
     1981 gtgccgatgg tgagactcga actcacacgg ctttcgccac cgccccctca agacggcgtg
     2041 tctaccaatt tcaccacatc ggcccgccgg tcccgaactg aagcccccgg actgaagcca
     2101 cccccgaact gaaactaccg ggcgccgcaa gctgttcgcg tcaaccgccc gcgttcactt
     2161 cgggatttcc tgcgctttcg agtcgccccc gggcgtggaa tccggcggct cggcatccgg
     2221 cggcgccgcc ggctgcggca cgtcgcccgc gggtgcggcc ggcgtcgccg gctgctcgac
     2281 cttgtgcgca tccatcacgc tgcccgtctc gggcttgcgc gccgcgtacc aggtgagccc
     2341 caggctggtc aggaaaaaca ccgtcgccag tacggctgta ctgcggctca ggaagttggc
     2401 cgagccgctg gcgccgaaca ggctgcccga cgcgccgccg ccgaacgccg cgcccatgtc
     2461 cgcgcctttg ccgtgctgga gcagcaccag cccgcagacg gcgatggcgg cggcgacgtg
     2521 cagcagaaga atcagaactt gcatcatgcc tcactatcgg tgcgatccgc cgcacggcag
     2581 atcgccatga actcgtcgag caccagcgac gcgccgccga ccaggccccc gtcgatgtcc
     2641 ggcatcgcga acagctctcc ggcgttcgcc cccttgacgc tgccgccgta gaggatcgtg
     2701 ctgcggtcag ccacctcccg gtcctgctca gcgatgcgcg cgcgcaggaa ggcgtgcacc
     2761 gcctgcgcct gctccggtgt cgcggtgcgg ccggtaccga tagcccacac cggttcgtaa
     2821 gccagcaccg cccgccccag cgcctgcacc ccgtggcgac cgatcgccgc tgccagctgc
     2881 gcggcgacca ccggctcggt ctcccccgct tcgcgttgcg ccagggtttc gccgacgcac
     2941 aggatgggca ccaaccccgt cgcaagcgcc gcgccgaact tggccgcgac cgtggcgtcg
     3001 tcctcgctgt acgcgacgcg ccgctccgag tgaccgacga tcgcgtaggt ggagccgaac
     3061 tccttcacca tcgccgcgga gacctcgccc gtgtaggcgc ctttcggatg ctgactgagg
     3121 ttctgggcgc cccacgcgac cgcgctgccg gccaaggctt gccgagcctg gaacagatag
     3181 ggaaacggca cacacaccgc gatgcgcgaa cgtctgagcg cgatggaccc gtgccgcaca
     3241 ccgcccagca gggccgcgtt ctccggaaga ctgccgtgca tcttccaatt cccggcgacc
     3301 agcttggcgc gcatgcccgc cttccaccca aatcggcgaa attttactct tggcgggggg
     3361 aggggtcaaa cggcctagcc gaacctgccg gtaatgtaat cctcggtcgc cttgttcttc
     3421 ggcttgatga agatggtatc ggtggcgtcg aactcgatca gctcgcccaa gtacatgtag
     3481 gcggtgtaat ccgacacgcg cgccgcctgc tgcatgttgt gcgtcacgat caggatggtc
     3541 agcttgctct tcagatcggc gatcagctcc tcgatgctcg ccgttgcgat cgggtccagc
     3601 gccgaggtcg gctcgtcgaa cagcaggatc tcggggtcgg tggcgagcgc ccgcgcgatg
     3661 cacaggcgct gctgctgtcc gccggagagg ttgaaggcca gttgcttgag ccggtctttc
     3721 acctcgtccc acagggcggc gctgcgcaac gcctgctcca ccttctcgtc gatcgccgcc
     3781 accttgcgca cgccgcgcac gcgtagccca taggcgacgt tctcgtagat cgatttcggg
     3841 aacgggttgg gcttctggaa caccatgctg atgcgcatgc gcacctcgat cgggtccacc
     3901 tgcgggtgca gcagattggt gtcgtccggg tacagcttga tctcgccctg gtagcggttg
     3961 cccgggtaga ggtcgtgcat gcggttgaag cagcgcaggt aagtggactt gccgcagccc
     4021 gacggaccga tcagcgccgt gaccttcctc tcgtacagcg gcatgctgat gctcttcagc
     4081 gcgtgatact gcccgtagtg gaagttcagc gccttggtct cggccttgat cgtcatcgcg
     4141 cccgatgcca tcgggttctc cgcgtcggtg gacctggttg gggttgcatc ctgtcgcatc
     4201 accacttgat ccgcttgcgc agctggtagc ggatgtagat cgcgagcgcg ttcatggaca
     4261 gggtcatgag cagcagcacc agaccggccg cggccgcgtt ctggtggaag gcggcctccg
     4321 gccgcgaggt ccagttgaac atctggatcg gcatcacggt gaacggcgcc atcagccatt
     4381 cgaacgacag gaaggggaat tccgcggtca ccggcgcggg cggcaggaag gcgatgaagg
     4441 tcagagcgcc gatggtgatg atcggggccg tttcgccgat cgcgcgcgcc atgccgatga
     4501 tcacgccggt catgatgccg ggcatcgcat agggtagcac gtggtcgagc gtggtctgcc
     4561 acttggtggc gcccaccgcg taggccgcct cgcggatcat cgacgggatg gagcggatcg
     4621 cctcccgggt ggcgacgatg accaccggca ggatgagcaa gcccagggtg agtcccgccg
     4681 cgaggatgct ctgcccgagg ccgaaggcgt acacgaacag ccccagcgcg agcaggccgt
     4741 agacgatgga cggcacgccg gcgaggttgg tgatgttgat ctcgatgatc gccgtgaacc
     4801 agttcttggg cgcgtactcc tcgaggtaca ctgcgctcgc cacgcccacc ggaatggcag
     4861 ccgcggcggt gaccaccatc accagcgtgc tgccgatcca ggcggacaga atgccggcct
     4921 gcgctggccg ccgcgacggg aagttggtga agaaatccca cgacaggcgc ggcgcgccgt
     4981 ccagcgccat gtccacgacc agggcggtga acgtgaccgc ggccacggcg aggcagagca
     5041 gacccacggc cgcgaacatc aggtcccagc gcttatgccg ggcgatgagc tcgcggatct
     5101 cccggatgga ggcgtgatcc atgcgcgcgc cctcgatcaa tagacctgcc ggaaccgccg
     5161 cgtcatgacg tggccgagga tgttgaacag cagcgtcatc agcatcagcg tgaggccggc
     5221 cgcgaagatg gtctgatagc cgatgctgcc gtggggcaga tcgcccaggg ccacctggac
     5281 gatgaacgag gtgatcgtgg ccgccggctc catcgggttc caggtgaggt tgggctgcat
     5341 gcccgcggcc accgccagga tcatggtctc gcccaccgcg cgcgagatgc ccaggatgta
     5401 cgcggcggtg atgccggaca gggctgcggg tgtgacgacc cggatagcgg tctggtagcg
     5461 cgtagcgccc atggcgtagc tgccctcgcg catgctcatc ggcacggcgc gcatcgcgtc
     5521 ctcgctgacc gagctcacgt aggggatgat catgatgccc atcaccagcc ccgccgacag
     5581 caggttgaag ccggggagcc ccgggatcag cttctgcagc agcggggtga cgaacagcag
     5641 cgcgaagtag ccgtacacca cggtgggcac gccggagagc agctcgagca ggggcttggc
     5701 ggcctcgcgc acggagaacg gcgcgaactc ggacagatag atggcgatga tggtgcccag
     5761 cggcaccgcc accgcgagcg ccaccgccga gctcaccagc gtgcccgaca gcagcaccat
     5821 gatgccgtag tgcgcgtcgt cgaacagcgg cgtccactgc gtgtcggtga ggaagtcgat
     5881 gatgctgaca tgctggaaga acagcaccga ttcggacacc agcacgtaca cgatgccgag
     5941 cgtgactgcc accgagaagg ccgcggcacc gaacaggatg aactcgatca agcgctcgcg
     6001 cagatgccgg gttctttttc tcgacagccg gtcgctgacg acgtagtctg ccaccgcgat
     6061 gagcccttct gtgtaggagg cggagggagg gggcatgggg ccttgttgcc tgcgagttta
     6121 cactagaagg cactgcggtc aggtgcacca aacaacgaga ggggcccgga cgcccctctc
     6181 gcgcgcgctg aagcaggggg gactgggtcg gcttagagct tcgcctccat tttcatcagg
     6241 tcttcgatgg tgagccccac cttcgcttcg ccgccgaact tcgtgccgac ctgtttcttg
     6301 gccaggtgtt ccatgttgta ggtgtacgcc ttggccggca gtggcacata cttcacctcg
     6361 cgcaccagga catcggcgtt cttcatgtag aactcgacga actgctgcac ctccggccgc
     6421 tccagcgact tggcgttgac gtagatgaag atcgggcgcg acaggggcgt gtaactgccg
     6481 ttgatcacgg cgtccataga cggtcccacg gcggtcttgc cgggctcggg agcgatgggc
     6541 accgccatca gcttgtcctg gttctcgatg tagtacgcga agccgaagaa accaagcgcg
     6601 ttgacgtcgc gcgacacgcc ctgcaccagc acgttgtcgt cctcgctggc ggtgaaatcg
     6661 ccgcggctcg ctttggactt gccgttgatc gcttcggtga agtagtcgaa ggtgccggaa
     6721 tctgccccgg gaccgaacag cttgagcggc tggtcaggcc aggcagcgtt gacctgcttc
     6781 cagctcttga ccttgccctg cgcggacggc tcccacatct tcttcagctc ggccggggtc
     6841 aggctcttga tccaggtgtt tttcgggttg atcaccacgg tgagcgcgtc gtacgccacc
     6901 ggcagctcgt agtactggat ccccgcggcc ttgcacgctt ccatctcgga cttgaggatc
     6961 gggcgcgagg cgtcggagat gtcggtttcg ccgcggcaga acttcttgaa cccgccgccg
     7021 gtgccggaga tgcccaccgt tacgcgtacc ttgcccttgg tggacttctg gaaatcctcg
     7081 gccatcgctt cggtgatcgg gaacacggtg ctggagccgt cgatcttcac cagctgcgcc
     7141 tgcgccggca gcgacagggc gccgatcgtt gcagcggcgc cggcggccag agcgatacgc
     7201 gagagacaat gcagtttcat cggagtcctc cttgtcggcg ggatgcgcat agtcgtcccg
     7261 attctaggcc ccgattgtga cggtttcatt gcatacggcc gcctaagcca cagagtacac
     7321 agagccgtgg tgacgaggtt cagaggcttg atatgctcag gcagcggcct gctggcggat
     7381 ggcctcggcg atgctctgcg ccaggccctg cacctcttcg gccgcttcgc cttccaccat
     7441 gacgcggatc accggctcgg tgcccgaggg gcgcagcaga acgcggccgc ggccttcgag
     7501 cgccacctcc gcggcacgca cggcgtcctg caccagcggg ttcttgcgga agtccgcgct
     7561 gcgctccacg cgcacgttga ccagcacctg cggatacaaa cgcaggccgc cgaggagctc
     7621 ggcgagcggc tggcgcttcg cacgcatcgc gtgcagcacc tgcagcgcgg agacgatgcc
     7681 gtcgccggtg gtgtgcttgt ccaggcagat gatgtggccg gagttctccc cgcccagctg
     7741 ccagccgcgc gcgcgcaatt cctccagcac gtagcggtcg cccaccgggg cgcgggcgaa
     7801 gggcaccgcc agggcggcga gcgcctgctc caagccgaag ttgctcatca gcgtccccac
     7861 cacgccgccg cgcaatccgc cgtcttcgag gcggcggcgc gcgatgacat agagcagctg
     7921 gtcgccgtcg taggtgcacc cgctgccgtc caccatcagc agccggtcgc cgtcgccgtc
     7981 cagcgcgagg ccgagatcgg cgccgtgctc cgccaccgcc gcgatcaacg ccgccggccg
     8041 ggtggcgccg caatccaggt tgatgttgag cccgtcgggc tggttgccga tcgccatcac
     8101 gtccgcgcca agctcgtgga acacgtgcgg tgcgatgtgg tagctcgctc cgtgcgcgca
     8161 gtccaccacg atcctgaggc cgcgcaggtc caggtcggtc gggaaggtgc tcttgcagaa
     8221 ctcgatgtag cgcccggcgg cgtcgttgat gcggcgcgcc ttgccgagct gggccggggc
     8281 ggcggtgacg atgggatcgc tcaggcgcgt ctcgatctcc tgctcggtgg cgtcgggcag
     8341 cttgtcgccg gcggcggaga agaacttgat gccgttgtcc tcgaacggat tgtgcgaggc
     8401 gctgatgacg atgcccgcgg acagccgcag cgcccgcgtc aggtaggcca ccgccggggt
     8461 cggcatggga ccgcacaggt agatgtccac gccggccgcc gacaggccgg cctgcagcgc
     8521 ggattccagc atgtagccgg agatgcgcgt gtccttgccg atcagcaccg ccggcggctc
     8581 gctgccggac gggcgccggt cggcgcccgc cagcaccttg ccggcggcgt atcccaaatg
     8641 catcaccagc tcgggagtga tcggcagctc cccgaccttg ccgcgcacgc cgtcggttcc
     8701 gaagtacttt ctgctcatcg ccgctgctcc cactggtcgc gctgcaacct gttttaccac
     8761 cgcggacacg cacggcacga agaacgcgaa agcaatgcaa aaccccgcgt gtccttcgtg
     8821 atcccgccgg ctgctcgacg ccgagcgcag gtcaacgttc ggcttcctgc aaggcgctca
     8881 gcaccagcag cgcatcgcgc gtggcggcca cgtcgtgcac gcgcacgatg gcggcgccgc
     8941 gctgcaccgc cagcagcgcc gcggcgacgc tcgccgccag gcgatcgccc ggcgcgcgcc
     9001 cggttatctc acccagcgtc gtcttgcgcg agagcccggc gagcaccggc atgccgagct
     9061 gcgccagctc gcccagccgg cgcagcagct cgagattgtg cgcggcggtc ttgccgaagc
     9121 cgaagcccgg atcgaccgcc agccgctcac ggtctatccc ggccgcggag caggcggcaa
     9181 cccgctgcgc gagaaattcc ttcacttccc gcatcacgtc cgtgtaagcc ggcgccacct
     9241 gcatggtctg cgggtcgccc tgcttgtgca tcaggcacag tccgcaatcc gagtcgcgca
     9301 ccgcgtccag agcaccgggc gcctgcaagg cgtcgatgtc gttgatcatc gacgcaccgt
     9361 ggctcaatgc cgcgcgcatc gtctcgggct tgcgcgtatc caccgagatc ggcaagggca
     9421 agccgcgtac cgcgtcgagc accggcagca cccgcgccgc ctccgcctcc gcggacaccg
     9481 gcgcggcacc gggccgcgtc gattcggcgc ccagatcgag gatgtccgcg ccctcctctg
     9541 ccagccggct cgcgtgctcg gcggccgcgg ccggatcgag gaaccggccg ccgtcggaaa
     9601 acgagtcggg cgtgatgttc accaccccca tgatgagcgg gcgggtcaac ggcagcagga
     9661 agcggccaca gcgcaaccgt ggcgaagccg cgggagcgcg cgccccctgc atcacttcgg
     9721 ttctggggcc ggggtcccgg tcggcgcggg cgcgctgggt ccgctgtcgc gcgggctcgg
     9781 cg
//
LOCUS       DASPGE010000334        10024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_906789, whole genome shotgun sequence.
ACCESSION   DASPGE010000334 DASPGE010000000
VERSION     DASPGE010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10024
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906789"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /gene="purH"
                     /locus_tag="VD791_10730"
     CDS             <1..1176
                     /gene="purH"
                     /locus_tag="VD791_10730"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HXV08494.1"
                     /translation="GPTMVRAAAKNHRHVAVVTDPRDYAQVLAELEEAGTLAHATRFR
                     LARKAFSHTAAYDGAIGNYLTAIGADGQRGDFPDQINFSFAKVQDLRYGENPHQKAAF
                     YAELERVPGALSGYSQLQGKELSYNNLADADASWECVKTFDAAACAIVKHANPCGVAL
                     GKDPLEAYQRALATDPTSAFGGIIAFNRELDPAVAQALSQQFVEVVIAPRVSAPARGV
                     LAAKQNMRVLEVPLEPGANRYDLKRVGGGLLVQTPDTRNVGAGELRVVTKAQPAPQQL
                     SDLLFAWRVAKFVKSNAIVFCRDGMTVGVGAGQMSRVDSARIAAIKAQNAGLTLAGSV
                     VASDAFFPFRDGLDVVVGAGARAVIQPGGSVRDDEVVAAADEHGIVMVFTGVRHFRH"
     gene            1199..2482
                     /gene="purD"
                     /locus_tag="VD791_10735"
     CDS             1199..2482
                     /gene="purD"
                     /locus_tag="VD791_10735"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HXV08495.1"
                     /translation="MNLLVIGSGGREHAIAWRLAQSPRIARVYVAPGNAGTAREDGLF
                     NVPLTGIDELVAFAWRESVLLTVVGPEAPLAAGAVDAFRAAGLKIFGPTRRAARLETS
                     KDFAKAFMAQQGVPTGAHCSFTDAAAAHDYLEQRDAPIVIKADGLAAGKGVVVAENAD
                     EAHRAVDAMLVDRRMGEAGAKVVIEEFLRGEEASFIVMVDGRNVLPLASSQDHKRLLD
                     GDKGPNTGGMGAYSPAPVLTPALHARAMREVIQPVVAGMEAMGEPYTGFLYAGLMITP
                     EQQLKVLEFNCRLGDPETQPILMRLKSDLFALIEHAVNGHLDRCEAQWDRRVALGVVL
                     AAAGYPEEPRKGDAIGGLPRNEEDFHVFHAATALADGTLVTNGGRVLCVTALGDTVRF
                     AQRRAYEIAERIQFAGKQMRRDIGQRAIDARRSPL"
     gene            2490..3398
                     /gene="hemF"
                     /locus_tag="VD791_10740"
     CDS             2490..3398
                     /gene="hemF"
                     /locus_tag="VD791_10740"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HXV08496.1"
                     /translation="MHPAAVRDYFADLQSRIVERLERLDGLPFRRDSWQRTEGGGGLS
                     CLIEEGRLFERAGVNFSHVTGSRLPPSATAARPELAGRSFQAMGVSLVLHPRNPYVPT
                     VHMNVRFLVAEEAAAQPVWWFGGGMDLTPYYGFEEDAVHFHRTCRAALAPFGPDWHPR
                     FKRWCDEYFHLRHRGEPRGVGGIFFDDLAEPGFDTSFALVRSVGDHFLDAYAPIVERR
                     RQIGYGARERDFQAYRRGRYVEFNLVYDRGTLFGLQSGGRTESVLMSLPPIVNWRYDW
                     HPAPGTPEAQLCEVFLKPRNWADMSS"
     gene            3492..3839
                     /locus_tag="VD791_10745"
     CDS             3492..3839
                     /locus_tag="VD791_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08497.1"
                     /translation="MKRRYLATVLLSLGLAAPVIAGEVDKGAVIGGAVGGGVGAGVGS
                     KVGGTSGAVIGGAIGGATGAAIGSSSTKEPEKVVVKERVVEKVVSVVEVEKPASPPGH
                     SRGKKKGWKKHRD"
     gene            complement(3903..4613)
                     /locus_tag="VD791_10750"
     CDS             complement(3903..4613)
                     /locus_tag="VD791_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV08498.1"
                     /translation="MSLLEVRRLRISYGGINAVKGIDLKVERGEMVALIGANGAGKTT
                     TLRAICGIVQPAAGKVHYDGADVTGAPSYSLVERGLALVPEGRGVFGRLTVEENLQMG
                     AYARRDAAGVRRDLERTYDLFPRLRERRRQSGGTLSGGEQQMLAIGRALMSAPKLLLL
                     DEPSMGLAPLMVQRIFDTVRRVAAEGVTVLLVEQNAKVALELSARGYVMESGQITLAD
                     SAPGLLNNPQVRQAYLGE"
     gene            complement(4610..5377)
                     /locus_tag="VD791_10755"
     CDS             complement(4610..5377)
                     /locus_tag="VD791_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV08499.1"
                     /translation="MMALLEARKLSKAFGGLQALSEVSLAIERGEIYGLIGPNGAGKT
                     TMFNVLTGLYKPSSGSFLFNGTELTGRKPHVIVEAGIARTFQNIRLFQHMTAVENAMV
                     GRHLRTHAGALGAVLRNARTRAEEQGIRRRAHELLDYVGVGRHAHAQAKNLSYGDQRR
                     LEIARALATEPKLLALDEPAAGMNATETVALRGLLEQIRADGVTILLIEHDVKLVMGL
                     CDRVAVLDYGRKIAEGAPAQVQKDPAVIEAYLGGALP"
     gene            complement(5374..6414)
                     /locus_tag="VD791_10760"
     CDS             complement(5374..6414)
                     /locus_tag="VD791_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV08500.1"
                     /translation="MLLVGVALALLPFVAEATLSRTWVRIIDFALLYAMLALGLNIVV
                     GFAGLLDLGYIAFYAVGAYTYALLASPHFDLHLPFWALLPLGALCAAVFGVLLGAPTL
                     RLRGDYLAIVTLGFGEIIRIFLNNLNAPVNITNGPQGVNRIDPLQVGDFAFNRSNEIF
                     GIVFPATHLYYYVFLALALLAMFVCVRLEDSRVGRAWIAIREDEVAAQAMGINTRNIK
                     LLAFAMGATFGGVAGGLFSAFQGFVSPESFVVMESIMVVCMVVLGGMGHVAGVVLGAM
                     LLATLPEVLRHTAVPVQKQLFGEVLIDPESLRMLLFGVALILVMLLRPAGLWPSPIRR
                     RELTSAAQPAKP"
     gene            complement(6518..7447)
                     /locus_tag="VD791_10765"
     CDS             complement(6518..7447)
                     /locus_tag="VD791_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV08501.1"
                     /translation="MDIFLQQVLNGLVLGSVYALVALGYTMVYGILGLINFAHGDVVM
                     VGALTALSVITTLVAAGVGLPAPVILLLALAVSMSVCMALGFTIERVAYRPLRRAPRL
                     APLITAIGVSILLQTLAMMYWGRRYHTFPQIVKPTRHEFLGAAITDVQILIVLVSASI
                     MGGLLLMVHFTRLGRAMRATSENQLIAGLMGVNINRIISATFIIGSALAAVAGVLVSA
                     NYGVAQYTMGFMLGLKAFTAAVLGGIGNLGGAMLGGLLLGLIESIGAGYMGDLTGGFL
                     GSHYQDVLAFVVLIGVLVLRPTGLLGERVADRT"
     gene            complement(7545..8705)
                     /locus_tag="VD791_10770"
     CDS             complement(7545..8705)
                     /locus_tag="VD791_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HXV08502.1"
                     /translation="MTTKQRGMTQRNVAVALAALAVLGLAGCGKKEEGVVKIGHAGPL
                     TGSIAHLGKDNENGVRMALDEANAAGAEIGGSKVKFELLSEDDQADPKQGTIVAQKLV
                     DAKVAGVIGHLNSGTTIPASKLYHDAGIAQISPSATNVTYTAQNFKTAFRVIANDAQQ
                     GKVLGEFATAKLGAKNVAIIDDRSAYGQGLADEFEKAATAGGATILAREFTDTTKTDF
                     TAILTSIKGKNPDLIFYGGMDAQAGPMMKQIQNLGVTATYLAGDGVQTAEFLKLAADS
                     AEGVYASSPGVPLDQMPQGPAFAEKYKAKFNQEIQIYAPYAYDAMNAMLEAMKKAGST
                     DPAKYLPELANIQYQGVTGNIAFDEKGDVRGGGISLYQVKGGKWEYIETIGG"
     gene            complement(8892..9212)
                     /locus_tag="VD791_10775"
     CDS             complement(8892..9212)
                     /locus_tag="VD791_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV08503.1"
                     /translation="MRKFLIAVVALAGVLGGAVTANAQADKAKQLAQKYNCLACHAED
                     KKLVGPAYKEIAKKYKGDAGAAAKLHAKIKQGGGGVWGTIPMPPNNVPDAEIKTMVDW
                     ILAM"
     gene            complement(9296..>10024)
                     /locus_tag="VD791_10780"
     CDS             complement(9296..>10024)
                     /locus_tag="VD791_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08504.1"
                     /translation="DWLESARRGLDFLRGVLWSKGRLLATCKDGRSHLNAYLDDHAFL
                     LAALLEMLQADFRKADLEFAVAIADALLGRFEDRAAGGFFFTSHDHERLILRPKTGYD
                     NATPSGNGVATLALQRLGHLLGDARYLEAAERSLRLFLPQFERHPSGLSTLLMALAEH
                     VQAPEVVILRGPRAAALPWQRALAAHYAPARLSLFVDPSLTGIPQALDKPLASAVNAW
                     VCRGVQCLPPIGDMAELLRELRAG"
BASE COUNT         1597 a         3477 c         3375 g         1575 t
ORIGIN      
        1 gggccgacca tggtgcgcgc agccgccaag aatcaccggc acgtggccgt cgtcaccgat
       61 ccgcgcgact acgcgcaggt gctggcggag ctggaggagg ccggcacgct cgctcatgcc
      121 acgcgcttcc gtctcgcgcg caaggcgttc tcgcacaccg ccgcgtacga cggcgccatc
      181 ggcaactatc tcaccgcgat cggcgccgac ggacagcgcg gcgacttccc cgaccagatc
      241 aatttcagct tcgccaaggt gcaggacttg cgctacggcg agaacccgca ccagaaggcc
      301 gccttctacg ccgagctcga acgcgtcccc ggcgcgctgt cgggctacag ccagctgcag
      361 ggcaaggagt tgtcctacaa caacctcgcc gacgcggacg cgtcctggga gtgcgtcaag
      421 accttcgacg ccgcggcgtg cgcgatcgtc aagcacgcca acccttgcgg tgtggccttg
      481 ggcaaggatc cgctcgaggc ctaccagcgc gcgctcgcca ccgatcccac gtcggccttc
      541 ggcggcatca tcgccttcaa ccgcgagctg gaccctgccg tggcgcaggc cctgtcgcag
      601 cagttcgtgg aagtggtgat cgctccgcgc gtgtcggcgc cggcgcgcgg cgtgcttgcc
      661 gcgaagcaga acatgcgcgt gctggaagtg ccgctggagc ccggggcgaa ccgttacgac
      721 ctcaagcgcg tgggcggcgg gctgctggtg cagacgcccg acacgcgcaa cgtcggcgcc
      781 ggcgagttgc gcgtggtgac caaggcgcaa cccgcgccgc aacagctgtc ggatctgctg
      841 ttcgcgtggc gcgtggccaa gttcgtgaag tccaacgcca tcgtgttctg ccgcgacggc
      901 atgaccgtgg gcgtgggcgc cggtcagatg agccgggtgg actccgcgcg catcgccgcc
      961 atcaaggcgc agaacgcggg tctcacgctg gccgggtcgg tggtggcctc ggacgcgttc
     1021 ttcccgttcc gcgatggcct cgacgtggtc gtgggcgccg gcgcgcgcgc ggtgatccag
     1081 cccggcggca gcgtgcgcga cgacgaagtc gtcgccgcgg ccgacgagca cggcatcgtc
     1141 atggtgttca ccggcgtgcg gcatttccgg cattgagcgc aaggcacctt tcgtcgacat
     1201 gaacctgctc gtcatcggca gcggcggacg cgagcacgcc atcgcctggc ggctggcgca
     1261 atcgccgcgc atcgcgcgcg tgtacgtggc accgggcaac gccggtaccg cccgggaaga
     1321 cgggctgttc aacgtccccc tcaccggcat cgacgaactg gtggccttcg cctggcgcga
     1381 gtcggtgctg ctcaccgtgg tcgggcccga ggccccgctc gcagcgggcg cggtggacgc
     1441 cttccgcgcc gcgggcctga agatcttcgg tcccacgcgc cgcgccgccc ggctcgagac
     1501 ctccaaggac ttcgccaagg ccttcatggc gcagcaaggc gtcccgaccg gcgcccactg
     1561 cagcttcacc gacgcggccg ccgcgcacga ctacctcgag cagcgggatg cgccgatcgt
     1621 gatcaaggcc gacggtctgg cggcaggcaa gggcgtggtg gtcgcggaga acgccgacga
     1681 ggcgcatcgc gccgtggacg cgatgctggt ggatcgccgc atgggtgagg ccggcgccaa
     1741 ggtggtgatc gaggagttcc tgcgcggcga agaggcgagc ttcatcgtca tggtggacgg
     1801 gcgcaatgtg ctgccgctcg cctccagcca ggatcacaag cgcctgctcg acggcgacaa
     1861 ggggccgaac accggcggta tgggcgctta ctcgccggcg ccggtgttga ccccggcgct
     1921 gcacgcgcgc gccatgcgcg aagtgatcca gcccgtggtc gccggcatgg aagccatggg
     1981 cgaaccgtac accggctttc tctacgccgg cctgatgatc acgcccgagc agcagctcaa
     2041 agtgctggag ttcaactgcc gcctcggcga tccggagacg caaccgatcc tgatgcggct
     2101 caagagcgac ctgttcgcgc tgatcgagca cgccgtcaac ggccatctcg accgctgcga
     2161 agcgcagtgg gaccggcgcg tggcgctggg ggtggtgctg gccgccgccg gctatcccga
     2221 agagccgcgc aagggcgacg cgatcggcgg gctgccgcgc aacgaggaag atttccacgt
     2281 gttccacgcc gccactgcgc tcgccgatgg taccctggtc accaacggcg gacgcgtgct
     2341 gtgtgtcacc gcgctcggcg acaccgtgcg cttcgcgcag cgccgcgcct acgagatcgc
     2401 cgagcgcatc cagttcgccg gcaagcagat gcggcgcgac atcggccagc gcgccatcga
     2461 cgcgcgccgc agcccgctgt aacacgccga tgcatcctgc ggcggtccgc gactacttcg
     2521 ccgatctgca atcccggatc gtcgagcggc tcgagcggct cgatggcttg cccttccgcc
     2581 gcgacagctg gcagcgcacc gagggcggcg gcgggctgag ctgcctgatc gaagaagggc
     2641 gcctgttcga gcgcgccggg gtcaacttct cgcacgtgac cggctcgcgc ctgccgcctt
     2701 cggccaccgc cgcgcgtccc gagcttgccg ggcgcagctt ccaggcgatg ggcgtgtcgc
     2761 tggtgctgca cccgcgcaat ccgtatgtcc cgacggtgca catgaacgtg cgctttctgg
     2821 tcgccgagga ggcggccgcg cagccggtgt ggtggttcgg cggcggcatg gacctcacgc
     2881 cctattacgg cttcgaggag gacgcggtgc acttccaccg cacctgccgc gccgcgctgg
     2941 cgccgttcgg ccccgactgg catccgcgct tcaagcgctg gtgcgacgaa tacttccacc
     3001 tcaggcaccg gggcgagccg cgcggggtgg gcggcatctt cttcgacgac ctcgccgagc
     3061 ccggcttcga caccagcttc gcgctggtgc gcagcgtcgg cgatcatttc ctcgacgcct
     3121 acgcgcccat cgtcgagcgc cgccggcaga tcggctacgg cgcgcgcgaa cgcgattttc
     3181 aggcgtatcg ccgcggacgc tacgtcgagt tcaacctggt gtacgaccgc ggcacgttgt
     3241 tcggcctgca gtccggcggc cgcaccgaat ccgtcctcat gtcgctgccg ccgattgtca
     3301 attggcgtta cgactggcat cccgcgcccg gcacacccga ggcgcagttg tgcgaagtgt
     3361 ttctcaagcc gcgcaattgg gccgacatgt ccagctgata aagcatgtcg gtgcctcacg
     3421 cttcacgcct cgccctacgg gcacaggtct tgcagccgct ttgcgccgtc atctcgacgc
     3481 aaggagcagc aatgaagcga cgttacctgg cgacggtcct tctctcgctc ggcctcgcag
     3541 cgccggtgat cgccggcgaa gtcgacaagg gcgcggtcat cggcggcgcg gtcggcggcg
     3601 gcgtgggtgc gggggtggga tccaaggtcg gcggcaccag cggtgcggtg atcggcggtg
     3661 cgatcggcgg cgcgaccggc gcggccatcg gctccagcag cacgaaggag ccggagaagg
     3721 tggtggtgaa ggagcgcgtg gtcgaaaaag tagtgagtgt ggtggaggtc gaaaagcccg
     3781 ccagcccgcc cggccacagc cgcggcaaga agaaaggctg gaagaagcat cgcgattgac
     3841 gtctgcgggc gcaacgcggc cacgcaagcg gcccgcagcg agcgggccgc ttgcatttgc
     3901 gctcactccc ccaggtacgc ctgacgcacc tgcggattgt tgagcagccc gggcgcggaa
     3961 tcggccagcg tgatctggcc gctttccatc acataaccgc gtgcgctcag ctcgagcgca
     4021 accttggcgt tctgctccac cagcagcacg gtgacgccct cggccgccac ccggcgcacg
     4081 gtatcgaaga tccgctgcac catgagcggc gccaggccca tgctcggctc gtccagcagc
     4141 agcagcttgg gcgcgctcat caacgcgcgc ccgatggcga gcatctgctg ctcgccaccg
     4201 gagagcgtac cgccggactg ccggcgtcgc tcgcgcaagc gcggaaacag gtcgtaggtg
     4261 cgctcgaggt cgcgcctcac ccccgcggcg tcgcggcgtg cataggcgcc catctgcaga
     4321 ttctcctcga ccgtcagccg gccgaacacg ccgcgtcctt cgggcaccag cgccaggccg
     4381 cgctccacca ggctgtagga gggcgcgccg gtgacgtccg cgccgtcgta gtgcactttg
     4441 cccgccgccg gctgcacgat gccgcagatc gccctcagcg tggtggtctt gccggcaccg
     4501 ttggcgccga tcagcgccac catttcgccg cgttcgacct tcaggtcgat gcccttgacg
     4561 gcgttgatac cgccgtagga gatgcgcaac ctgcgcactt ccagcaggct cacggcagcg
     4621 cgcccccgag gtaggcctcg atcaccgccg ggtctttctg cacctgcgcc ggcgcgccct
     4681 cggcgatctt cctgccgtag tcgagcacgg cgacgcgatc gcacagcccc atcaccaact
     4741 tgacgtcgtg ctcgatcaac aggatggtca cgccgtccgc gcggatctgc tccagcaggc
     4801 cgcgcagcgc gaccgtttcg gtggcattca tgccggcggc cggctcgtcg agcgccagca
     4861 gcttgggttc ggtggcgagc gcacgcgcga tctcgagccg gcgctgatcg ccgtaggaca
     4921 ggttcttcgc ttgcgcgtgc gcgtgacggc cgactccgac gtagtcgagc agctcgtgcg
     4981 cgcgccgccg gatgccctgc tcctcggcgc gcgtcctggc gttgcgcagc accgcgccca
     5041 gcgcccccgc gtgcgtacgc agatggcgtc ccaccatggc gttctcgacc gccgtcatgt
     5101 gctggaacag gcggatgttc tggaacgtgc gcgcgatgcc ggcctcgacg atgacgtgcg
     5161 gcttgcgccc ggtcagctcg gtgccgttga acaggaagct gccggagctg ggtttgtaca
     5221 ggccggtcag cacgttgaac atggtggtct tgccggcgcc gttggggccg atcagcccgt
     5281 agatctcgcc gcgctcgatg gcgagcgaga cctccgacag cgcctgcagg ccgccgaagg
     5341 ccttgctcag cttgcgcgct tccagcagcg ccatcaaggc ttggcgggct gcgccgcgct
     5401 ggtgagctcg cggcggcgta tcggcgaggg ccacaggccc gcggggcgca gcagcatcac
     5461 caagatcagc gccacgccga acagcagcat gcgcaggctc tcgggatcga tcagcacctc
     5521 gccgaacagc tgcttctgca ccggcaccgc ggtgtgacgc agcacctcgg gaagcgtggc
     5581 caggagcatg gcgcccagca ccacgcccgc cacgtgaccc atgccgccga gcaccaccat
     5641 gcacaccacc atgatcgatt ccatcaccac gaagctctcc gggctgacga agccctggaa
     5701 ggccgagaac aggccgccgg ccactccgcc gaaggtcgcc cccatcgcga acgcgagcag
     5761 cttgatgttg cgcgtgttga tgcccatcgc ctgcgccgcg acctcgtctt cgcggatggc
     5821 gatccaggcg cggcccacgc gcgaatcctc caggcgcacg cagacgaaca tcgccagcag
     5881 cgccaacgcc aggaacacgt agtagtacag gtgggtggcg gggaacacga tgccgaagat
     5941 ctcgttgctg cggttgaagg cgaaatcgcc cacctgcagc gggtcgatcc ggttgacgcc
     6001 ctgcggcccg ttggtgatgt tgaccggcgc attgagattg ttcaggaaga tgcggatgat
     6061 ctccccgaag ccgagggtga cgatggccag gtaatctccg cgcaggcgca aggtcggcgc
     6121 gcccagcagc acgccgaaca ccgcggcgca cagcgcgccc aggggcagca gcgcccaaaa
     6181 cggcagatgc agatcgaaat gcggcgaagc gagcagcgcg taggtgtagg cgcccacggc
     6241 atagaaggcg atgtaaccga ggtcgagcag cccggcgaac ccgaccacta tgttcaggcc
     6301 gagcgccagc atggcgtaga gcagcgcaaa gtcgatgatg cgcacccagg tgcgcgacag
     6361 ggtcgcctcc gccacgaacg gcagcagcgc gagcgccacg cccaccagca gcatgccgat
     6421 ccagggcgca tgccgatggc gcttgatggc atgccagtga ccggcgagag ctttcacgcg
     6481 ggcctcgcgc cccgtgcctc gtgcctcgtg cccggggtca cgtccggtcc gccacgcgtt
     6541 cgcccagcag cccggtgggc cggagcacga gcacgccgat cagcaccacg aaagccagca
     6601 cgtcctgata gtggctgccc aggaaaccgc cggtaagatc gcccatgtag cctgctccga
     6661 tgctctcgat cagtcccagc agcagcccgc ccagcatggc gccgccgagg ttgccgatgc
     6721 cgccgagcac ggccgcggtg aacgccttca aacccagcat gaagcccatg gtgtactgcg
     6781 cgacgccgta attggcgctc acgagcacgc cggcgacggc cgccagcgcc gagccgatga
     6841 tgaacgtggc ggagatgatg cggttgatgt tgacccccat cagccctgcg atcagctggt
     6901 tctcggaggt ggcgcgcatc gcgcggccga gccgcgtgaa gtgcaccatc agcagcaggc
     6961 cgcccatgat cgacgccgac accagcacga tcaggatctg cacgtcggtg atggcggcgc
     7021 cgaggaactc gtgccgggtg ggtttgacga tctgcggaaa ggtgtgatag cgccgccccc
     7081 agtacatcat ggcgagcgtc tgcagcagga tcgagacgcc gatggcggtg atcagcgggg
     7141 ccaggcgcgg cgcgcggcgc agcgggcggt aggcgacccg ttcgatggtg aaaccgagcg
     7201 ccatgcacac cgacatggac accgccaggg cgagcagcaa gatcaccggg gcgggcaggc
     7261 ccaccccggc cgccaccagc gtggtgatca ccgacagcgc ggtgagcgcc ccgaccatca
     7321 ccacgtcgcc atgcgcgaag ttgatcaggc cgaggatacc gtagaccatg gtgtagccca
     7381 gggccaccag cgcgtagacg ctgcccagga ccaggccgtt cagcacttgc tggagaaaga
     7441 tgtccataaa aaacggcgcc gcccctcgac cgggagcgca gcgccgtcta gaagtccctg
     7501 cggctcaccc gcgggtgcag cctgtcgcgg gcgccgtcaa gtgttcagcc gccgatggtc
     7561 tcgatgtact cccacttgcc gcccttgacc tggtacaggc tgatgcctcc gcctctgacg
     7621 tcgcccttct cgtcgaaagc gatgttgccg gtgacgccct ggtactggat gttggccagc
     7681 tcgggcaggt acttggcggg atcggtggaa ccggccttct tcatcgcttc gagcatcgcg
     7741 ttcatggcgt cgtaggcgta aggcgcgtag atctggatct cctggttgaa cttggccttg
     7801 tacttctcgg cgaaggccgg gccctgcggc atctggtcca gcggcacgcc gggagacgaa
     7861 gcgtaaacgc cctcggcgga gtcggcggcg agcttgagga actcggcggt ctgcacgcca
     7921 tcgccggcca ggtacgtcgc ggtgaccccc agattctgga tctgcttcat catcgggccg
     7981 gcttgcgcgt ccatgccgcc gtagaagatc aggtcggggt tcttgccctt gatgctggtc
     8041 aggatcgcgg tgaagtcggt cttggtggtg tcggtgaact cgcgcgcgag gatggtcgcg
     8101 ccaccggccg tggcggcctt ctcgaactca tcggccaggc cctggccgta ggccgagcgg
     8161 tcgtcgatga tggcgacgtt cttggcgccc agcttggcgg tggcgaactc gcccagcacc
     8221 ttgccctgct gcgcgtcgtt ggcgatcacg cggaaggcgg tcttgaagtt ctgcgcggtg
     8281 taagtgacgt tggtggccga gggcgagatc tgcgcgatgc ccgcgtcgtg atacagcttg
     8341 gacgcgggga tcgtggtgcc ggaattgagg tgtccgatca cgcccgcgac cttggcatcc
     8401 accagcttct gggcgacgat cgtgccctgc ttcgggtccg cctggtcgtc ctcggacagc
     8461 agctcgaact tgaccttgct gcccccgatc tctgcgccgg cagcgttggc ctcgtcgagc
     8521 gccatgcgca cgccgttctc gttgtcctta cccagatggg cgatggagcc ggtgagcgga
     8581 ccggcgtgcc cgatcttcac caccccttcc tctttcttgc cgcagcccgc cagtcccagc
     8641 acggcaagcg ccgccagcgc caccgccacg ttcctttgcg tcatcccccg ctgtttcgtt
     8701 gtcatgcttt ctcctcggcg taacggtcgg cccgtcaacg gcgcggatta ttctccctcc
     8761 ccatgggatt gtcaaattct ccgcagattc atcggcttgc ccttgctcac cgggcccgga
     8821 cggaaaaact aaaaaagccg gcgcaggaag cgccggctct gtctgccgga ctgggactgc
     8881 cttcagccga gctacatggc gaggatccaa tccaccatgg tcttgatctc ggcatccgga
     8941 acgttgttcg gcggcatggg aatggttccc cacaccccgc caccgccctg cttgatcttg
     9001 gcgtgcagct tcgccgccgc accggcatcg cccttgtact tcttggcgat ctctttgtaa
     9061 gcgggcccca ccagcttctt gtcctcggcg tgacaggcca ggcagttgta tttctgcgcc
     9121 agctgcttgg ccttgtcggc ctgggcattg gcggtcaccg cgccgcccag caccccggcc
     9181 agggcaacca ccgcgatcag gaactttctc atggtcacca aaccccttcc ctccttggtc
     9241 gttgagcgca aagcaggcgc gattgtaaag ccttttgcgc gccctttggg cgcgatcacc
     9301 cggcgcgcag ttcgcgcaga agctccgcca tatcgccgat cggcggtaaa cactgaacgc
     9361 cgcggcacac ccacgcgttg acagcgctgg cgagcggttt gtccagcgcc tgcgggatgc
     9421 cggtcaggga cgggtccacg aacaggctca accgggcggg cgcgtaatgc gccgccagcg
     9481 cgcgctgcca gggcagggcc gcggcgcgcg ggccgcgcag gatcaccacc tcgggggcct
     9541 gcacgtgctc ggcaagcgcc atcagcagcg tcgacaatcc gctcgggtgg cgctcgaact
     9601 gcggcaggaa cagccgcaaa gagcgctcgg ccgcttccag gtaacgcgca tctcccagca
     9661 gatgcccgag gcgctgcagc gccagcgtgg ccacgccgtt gcccgaaggc gtggcgttgt
     9721 cgtagcccgt cttcggccgc aggatcagcc gctcgtgatc gtggctggtg aagaagaacc
     9781 cgcccgccgc gcggtcctcg aagcgtccca gcaaggcgtc ggcgatcgcc accgcgaact
     9841 cgaggtccgc cttgcggaag tcagcttgca gcatctccag cagcgccgcg agcaggaacg
     9901 cgtggtcgtc gaggtaggcg ttcagatgcg accggccgtc cttgcaggtc gccagcaacc
     9961 ggcccttgct ccagagcaca ccgcgcagga aatcgaggcc gcggcgtgcc gactcgagcc
    10021 agtc
//
LOCUS       DASPGE010000335        10759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1257950, whole genome shotgun sequence.
ACCESSION   DASPGE010000335 DASPGE010000000
VERSION     DASPGE010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10759
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257950"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VD791_10785"
     CDS             <1..528
                     /locus_tag="VD791_10785"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXV08505.1"
                     /translation="IYTAVGHSHAHGDVHMHSHGHAHGHDHGHAHDHEHGHDHGHGEA
                     PPGYHSHGWGFKHTHNVDLVASNRPSMWMLIGLGVAGGLLPDPAALAILLSALAQGKV
                     MLGLGTVLVFSVGFAATLVVVGVVAAIVGQRILTWLAGRTAARLQIATSLVIVLMGLW
                     LTAKGWRDVLVSSAL"
     gene            complement(563..1546)
                     /locus_tag="VD791_10790"
     CDS             complement(563..1546)
                     /locus_tag="VD791_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08506.1"
                     /translation="MTASVVAPPTARRGWLTAGVIFGLALAVLAFGLYRLANRHEFVE
                     YKMEHARDSPTAIAAAPDGSIWFTIDSASALGRVQDGKVIRLPKPTENLEPVGLAVAA
                     DGSAWFTDAPAKQVSRMTPTGELTSVTLEMPIARLGAIAVAPDGAVWFAEGSAYSVTR
                     LKDGALKRHVIESVRGGPYGVAVGPDGSAWVTLQAANQLLRIAPEGPMRAFDIPTRGS
                     APSDIRVDAQGVVWFLEFRSNKLGRLEDGKFQEFELAEHNVAPTGLAVAPDGAVWFGM
                     LRTGSLGRFQDGKIRTVKLPRENARPYSVTVDAAGNVWYADISGYVGMLRR"
     gene            complement(1558..2706)
                     /locus_tag="VD791_10795"
     CDS             complement(1558..2706)
                     /locus_tag="VD791_10795"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HXV08507.1"
                     /translation="MRLRHLLLAFALTLGAGPAPAHDPSAYGGLFRSRDLGQRWLNAD
                     VGLFLGAAVSVSIDPTDPNRLLLGTDSGLLISRNGGRKWDRVAPAKLFGAVFAVAFLP
                     DGRSALCATPGGVYRLEDGDWQQVAAPTEAAPARAIAFGAAPGRVYLIGRRDLYRSDD
                     GGRSFLEVEHGLPDRPEITELAVSAQPEEVLVAVADGRPMVSRDGGRSWQQRSAGLPQ
                     SPAEGLALDPVVAGRLWIASASRIYRSDDAGMTWLAAGEPLPEADTSVRGIAADRSGG
                     VLVVTTHRGLYRSADAGRTWALLEDNLPVHLEARPLVRDVSSPETLYAGFSLIPYGEL
                     WRTALEGGNLLNRVDLLSLAGGLAFLLLLGLLGVLGARWLFRRDARTA"
     gene            complement(2773..3780)
                     /locus_tag="VD791_10800"
     CDS             complement(2773..3780)
                     /locus_tag="VD791_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV08508.1"
                     /translation="MRMWRVIKGAALGVAALGCMVPFASQAQQYSVWLVENSRFMNVP
                     YGAFMPDRAFVPGSTSKFNNINLIDIPVNVGVIKGTVNGKLEVVLADCGWKQQDYHKM
                     TGTAHWAPLPDQLKLLGISANQVTKIVAGHGHWDHAGSLSDFPNAKLYIQKKELEGIE
                     WALNYPHPRIAATNVSEGGCARTPGCGYPPKTLDEIYGKVLSGNAVIVDGEMEILPGI
                     RIHPAHHAHTPGSQLLEVRTGGSVGTLVFGSDVYSSWQGIRDWMVANIQSSTDSAQQF
                     LAYEKCYKLTNNNMGNCIAAHEPTSYTKDYPITANSWVGINGSRMAEITLAPGESSKK
                     K"
     gene            complement(3892..4494)
                     /locus_tag="VD791_10805"
     CDS             complement(3892..4494)
                     /locus_tag="VD791_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08509.1"
                     /translation="MPGQAVQSAQLPRRMGWKTFAVAALVLALGAFAAYALQRELRTK
                     PEAPSAAAAFTAGLAMGEQHAARALSAEEESYAAALWPIHSQVKLSAVRMTFAGLNYK
                     IEHNDPAKLKAAVQPLTRTFTDAAQRARKIEPPASLADAHRTYLEAIDHYAAASREMI
                     RIADDGREEHLFAAHKRSEQASHELLKLSDVLWPGEYKPN"
     gene            complement(4626..5465)
                     /locus_tag="VD791_10810"
     CDS             complement(4626..5465)
                     /locus_tag="VD791_10810"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08510.1"
                     /translation="MLTLLSLIAVGFFLGMRHATDADHVVAIATIVTRERSLRGAAAI
                     GALWGIGHGVTVAIVGCLIILFGVVIPPRVGLAMEFAVAVMLVLLGLFTLSAIARQAR
                     EALALHLLHGEHGAGHVPADADGHTHVHTHGDYVHAHAHGHGDGAHGHPEQSTPQAWL
                     DRHFGRLGLYQILRPVVVGVVHGLAGSAAVALLVLTAIRDPLWGLAYLAVFGVGTVAG
                     MMLITVAIAAPFAVGAKRLPLVSAGVRAAAGVLSLAFGLFLMYEVGVVGGLFSADPQW
                     QPR"
     gene            complement(5540..8119)
                     /locus_tag="VD791_10815"
     CDS             complement(5540..8119)
                     /locus_tag="VD791_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXV08511.1"
                     /translation="MSTLSVAAYWPLWLLALLPLAWWVSLRSRTNLGRGHLAVATALR
                     TLALVFLVLALMQPARTARISEISVVYALDVSRSVASGFVRSALDFARRANAQAEPGA
                     ARYLVFADRARLVDTAEDVVKVGVSAAVADRDRLLYQGATNLELALDQALLGLDPARV
                     PRVVLFTDGNQTRGDVWRALPRLTAHGVRVFAFPAPPQAQSDAWIEALDVPEGVRRDE
                     PVSLTVHVVSQRETPARITVSADGRALGARRLRLNAGSNVVTIATRIARSGSVIIGVA
                     VHAEGDLLADNDRLQQSAWIGPRPRVLYVEGQPEAAQYLSAALRREGLEVRAVGAEEL
                     PGELDALERFDAVILSDVAPDRIDAARMRALQRYVRESGGGLIFAAGDSTYGKDGFAD
                     TALERVLPVEFKSREKRKDLALVLCLDRSYSMKGRPLALAKAGARATLELLEEQHYFG
                     VVAFDSQPHEAVPLQYVRSKRRAEELLDRIQASGQTNIYPALASAWRMLQNHPAKRKH
                     VILISDGDTAPADFDRLLKRMTDSKITVSTVTIGRSGDPGLMERIAGFGGGRAYVAEN
                     LEQLPQLFVQDTQNVSRATLMEEPFRPVVKRRIEALRGLDFAKAPPLQGFASTKAKDG
                     AEVFLTADADAPLLARWQYGLGRSVMFASDVKNRWAAEWLQWEGYGKFWAQLTRDTLR
                     RDTGEQVLFRVVREGDEARVRLRLMTEQGGWRNGLAPRVRATGPDGSRRDLVLRQTGP
                     GHYDATLAVATAGSRPYTFAVVSGGGVGVETARRAGTRQLFYPYPDEYHSLPPDLELL
                     TALAEQTGGKVGASVTEIFADQGDRGRSRTPLWPWLAAVALALYLADLFVRRAPWVRR
                     WLE"
     gene            complement(8116..9942)
                     /locus_tag="VD791_10820"
     CDS             complement(8116..9942)
                     /locus_tag="VD791_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXV08512.1"
                     /translation="MRFLGLPAAGAWLLLAGTAGLVVLLYWLKPSARRLVVSSGLIWR
                     RVLESRKRRAERWRWWLSLMLALGIALAIAAALTRPEIAAVSGAAADVLLVIDTGPSM
                     AARGADGRTRLARALDSAEAVVRASGAGSRYLVADTTHQVATPALESREDALARLRTL
                     APRAGAQPWFPDVARPAGAGQRLQLWFFTDGVADFAVPREARLVSAFQMADNLGITAF
                     EVRALPADARRHEAYLEITNASLGDKRVEVQVVGVGAAPLERALQLRGGAAADVVLDL
                     SAFEEGPLRATLRTDTDALDLDNTAYTYLPGKGRVRVGLFTAGNPALAQALRLLPRVE
                     VALATPERWRDSVRFDAVVLDRFAPPRPPAVPALLIAPGPVSWLPRSATDASDIRLAR
                     WDAAHPLLANVSLRDVLVDRAPLLALDAPRDEAGSAQRLPLAPLARGARNEPLILATR
                     EGPRLALLSFALEASNFAQQASFPAFLSNAVDWLTREPRALAHRIGQVSVPSVQARVL
                     DLEGRAVQTRPAPDATLFDVAEPGLFTALTREQRVRIAVNALDPQLTAINASRFAWGS
                     APAAPPAPVPARLRIDPWMLLLALATILLAVEWWTYNRRVTV"
     gene            complement(9953..>10759)
                     /locus_tag="VD791_10825"
     CDS             complement(9953..>10759)
                     /locus_tag="VD791_10825"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXV08513.1"
                     /translation="YGQLRGMHRSRRVGTGMVFADYRPYSEGDDIRNIDWGIYLRLDR
                     LILRLFEEEADLPVYVFLDASQSMDHGKPNKLEFARKLAGALGYVALLNHDRVNLSAF
                     ADGLREMLPTRRGRSQAPQLFRFLQSLQPRGRTRLHAALRRYFSVPRTRGLVILISDF
                     LDPEGIEEALAVLRRFRHDVALLHVMAPDERDPQLPEEVVLVDAEEGTATEVEITPAL
                     LGAYRSTFEQHARDIEAYCRKYGWGYARALTDVPFEDLVLKVLRREGLLR"
BASE COUNT         1596 a         3851 c         3760 g         1552 t
ORIGIN      
        1 atctacaccg cggtcgggca ttcgcatgcg cacggcgatg tccacatgca ctcgcacggc
       61 catgcacatg ggcacgacca tggccatgcc catgatcacg agcacgggca cgaccatggg
      121 cacggcgagg ccccgcccgg ctatcacagc cacgggtggg gtttcaagca cacgcacaac
      181 gtggatctgg tcgccagcaa ccgcccgagc atgtggatgc tgatcgggct gggcgtggcg
      241 ggaggtctgc tgccggatcc cgcggcgctc gccatcctgc tgtcggcgct ggctcagggc
      301 aaggtgatgc tggggctggg cacggtgctg gtgttcagcg tggggttcgc cgcgacgctg
      361 gtggtggtgg gggtcgtggc cgcgatcgtg ggccagcgca tcctcacctg gctcgccggg
      421 cgcaccgcgg cacgcctgca gatcgccacc tcgttggtga tcgtgctgat ggggttgtgg
      481 ctgaccgcga agggctggcg agacgtgctg gtgagcagtg cgttgtgatc gggcggttgc
      541 gcttgccggt tacgcctttc gctcaccttc tcagcatgcc aacgtatccg ctgatgtcgg
      601 cgtaccagac gttgcccgcc gcatcgacgg tcacgctgta gggacgcgcg ttctcgcgcg
      661 gcagcttgac cgtgcggatc ttgccgtcct ggaagcggcc caagctgccg gtgcgcagca
      721 tcccgaacca caccgcgccg tcgggcgcga ccgccaggcc cgtgggcgcg acgttgtgct
      781 cggccagctc gaattcctgg aacttgccgt cttcgagccg gcccagcttg ttgctgcgga
      841 attcgaggaa ccacaccacg ccctgcgcgt cgacgcggat gtcgctcggc gcgctgccgc
      901 gcgtggggat gtcgaaggcg cgcatgggtc cctcgggtgc gatgcgcagc agctggttcg
      961 cggcctgcag cgtgacccag gcgctcccgt cgggcccgac cgccacgccg tacggcccgc
     1021 cgcgcacgga ctcgatcacg tggcgcttga gcgcgccgtc cttcaggcgc gtgacgctgt
     1081 aagcgctgcc ctcggcgaac cacaccgcac cgtcgggggc aaccgcaatc gctcccaggc
     1141 gcgcgatggg catctccagg gtcaccgagg tcagctcgcc tgtgggcgtc atgcgcgaca
     1201 cctgcttggc cggcgcatcc gtgaaccagg ccgatccgtc ggcggccacg gcgagtccga
     1261 ccggttccag gttctccgtc ggcttgggca gccggatcac cttgccgtcc tgcacacggc
     1321 ccagggcgct ggcggaatcg atggtgaacc agatgctccc gtccggcgcg gcggcgatcg
     1381 cggtggggct gtcgcgcgca tgctccatct tgtactcgac gaactcgtgc cggttggcaa
     1441 ggcgatacag accgaacgcc agcacggcga gcgccagtcc gaagatgacg ccggcggtaa
     1501 gccaaccccg gcgcgcagta gggggggcga caaccgatgc ggtcatgggt gcggtcctca
     1561 agcggtgcgg gcgtcgcgcc ggaacagcca gcgcgcgccg agcacgccca gcagcccgag
     1621 cagcaacaga aaggcgagcc cgccggcgag gctcagcagg tccacgcggt tgagcagatt
     1681 gccgccctcc aacgccgtgc gccacagctc gccgtacggg atcagcgaga agccggcgta
     1741 cagagtctcg ggactggaga catcgcgtac cagcgggcgc gcctccaagt gtaccggcag
     1801 attgtcttcg agcaacgccc aggtgcggcc ggcgtcggcg ctgcggtaca ggccgcgatg
     1861 tgtggtcacg acgaggacgc cgccgctgcg gtcggcggcg atgccgcgca cgctcgtgtc
     1921 cgcctcggga agcggctcgc cggcggccag ccatgtcata ccggcatcgt cgctgcggta
     1981 gatgcggctc gcgctcgcga tccacaagcg cccggccacg accggatcca gcgccaggcc
     2041 ctcggcggga ctttgcggca gaccggcgct gcgttgctgc caggagcgcc cgccgtcgcg
     2101 gctcaccatc ggccggccgt cggcgactgc aaccaggact tcctccggct gcgcgctcac
     2161 cgccagctcg gtgatctccg gtcgatccgg gagaccgtgc tcgacctcga ggaagctgcg
     2221 cccgccgtca tcgctacggt acagatcgcg ccggccgatc agatacacgc gtccgggtgc
     2281 ggcgccgaac gcgatggcgc gcgccggcgc cgcttccgtc ggggcggcca cctgctgcca
     2341 atcgccgtcc tcgagccggt acacgcctcc cggcgtcgcg cacagcgccg accgaccgtc
     2401 aggcagaaat gcgaccgcga acaccgcccc gaacagcttc gcgggcgcca cgcggtccca
     2461 cttgcgtccg ccgttgcgcg agatcaacag cccgctgtcc gtgcccagca gcaagcggtt
     2521 cggatcggtg ggatcgatgg acaccgatac ggcggcgccg aggaacagcc cgacgtcggc
     2581 gttgagccag cgctgcccga ggtcgcgcga gcggaacagg ccgccgtacg cgctcgggtc
     2641 atgcgccggc gccgggccgg cgccgagcgt cagcgcgaat gcaagcagga gatgacgaag
     2701 ccgcatggca aaaagaacga ggagccggga accccggctc ctcgcgggtg aactgcgcag
     2761 tgccggtcgg acctacttct tcttcgagct ctcgcccggc gcgagcgtga tctccgccat
     2821 gcgcgagccg ttgatgccga cccacgagtt ggcggtgatc gggtagtcct tggtgtagga
     2881 tgtgggctcg tgcgcggcga tgcagttgcc catgttgttg ttggtgagct tgtagcactt
     2941 ctcgtaggcc aggaactgct gcgcgctgtc ggtcgagctc tggatgttgg ccaccatcca
     3001 gtcgcgaatc ccctgccacg aagagtacac gtcgctgccg aacaccagcg tgcccaccga
     3061 cccgccggtg cgcacctcga gcagctgcga gccgggggtg tgcgcgtggt gcgccgggtg
     3121 gatcctgatc ccgggcagga tctccatctc gccgtccacg atcaccgcgt tgccgctcaa
     3181 caccttgccg tagatctcgt ccagggtctt gggcgggtag ccgcagcccg gggtacgggc
     3241 gcagccgcct tcggacacgt tggtcgccgc gatgcgcggg tgcggatagt tgagcgccca
     3301 ctcgatgcct tccagctcct tcttctggat gtagagcttg gcgttcggga agtcgctcag
     3361 gctgcccgcg tggtcccagt gcccgtgacc cgcgacgatc ttggtgacct ggttcgccga
     3421 gatgccgagc agcttgagct gatcgggcag cggcgcccag tgcgcggtgc cggtcatctt
     3481 gtgatagtcc tgctgcttcc agccgcaatc ggccagcacc acttccagct tgccgttcac
     3541 ggtgcccttg atgacgccga cgttgaccgg aatgtcgatc aggttgatgt tattgaactt
     3601 gctggtgctg ccgggcacga acgcgcggtc gggcatgaac gcgccgtagg gcacgttcat
     3661 gaagcggctg ttctccacca accacaccga gtactgctgc gcctgcgacg cgaacggtac
     3721 catgcagccg agagcagcca cgcccaacgc tgcgcccttt attaccctcc acattctcat
     3781 tcttggttcc tccatgtcac gcgtgatgaa gctgacgggc ttccgcccgg cgtcgcgctt
     3841 gtggcgctcg gccgtggtcc ccctcagttt tctgctcgat tcggcagttt gctagttcgg
     3901 cttgtactct cccggccaca gcacgtcgct caacttgagc agctcgtgcg aggcctgctc
     3961 gctgcgcttg tgcgcggcga acagatgctc ttcgcgcccg tcgtccgcga tccggatcat
     4021 ttcgcgggac gccgcggcat agtgatcgat cgcctccagg taggtgcggt gcgcatccgc
     4081 gagcgacgct ggcggctcga tcttcctggc gcgctgggct gcgtcggtga aggtgcgggt
     4141 gagcggctgg acggcggcct tcagcttggc ggggtcgttg tgctcgatct tgtaattcag
     4201 gccggcgaag gtcatgcgca cggccgagag cttgacctgg ctgtggatcg gccacagcgc
     4261 ggcggcgtag ctctcctcct ctgcgctcag ggcgcgcgcg gcgtgctgct cgcccatcgc
     4321 cagcccggcg gtgaaggccg cggccgcgga aggcgcttcc ggtttcgttc tcagctcgcg
     4381 ctgcagggcg tacgcggcaa aggcgccgag cgccagcacc agcgcggcga cggcaaaggt
     4441 cttccaaccc atcctgcgcg gcaattgcgc cgactggacc gcctgacccg gcatcccctc
     4501 ccccctcgtg cgcagccctg cgcggcggct gcgtcggtcg ttctcttgcg cgccaatgta
     4561 gcggcttcgc gcgggtatgg caactgcgtc accggacgct taattcccgg caagagatcc
     4621 tacgcctatc tcggctgcca ctgcgggtcg gccgagaaca gtccgccgac cacgcccacc
     4681 tcgtacatca ggaacaggcc gaacgccagg ctcaacaccc cggcggcggc gcgcacaccg
     4741 gcgctcacca gcggcaggcg cttggccccc accgcgaagg gcgcggcgat ggccaccgtg
     4801 atcagcatca tcccggccac cgtgcccacg ccgaacaccg ccagataggc caggccccac
     4861 aacgggtcgc ggatggcggt gagcaccagc agcgccaccg ccgccgagcc ggccaggccg
     4921 tgcaccaccc ccaccaccac cggccgcagg atctggtaga ggccgagcct gccgaagtgg
     4981 cggtccagcc atgcctgggg cgtggactgc tccggatgcc cgtgcgctcc gtcgccgtgg
     5041 ccgtgcgcgt gcgcgtgcac gtagtcgcca tgggtgtgga catgcgtatg cccgtcggcg
     5101 tcggcaggta cgtgccccgc accgtgctca ccgtgcagca gatgcagcgc cagcgcttcg
     5161 cgcgcctggc gcgcgatggc cgacagcgtg aacaggccca gcagcaccag catgacagcc
     5221 accgcgaact ccatggcgag gcccacgcgc gggggaatga ccacgccgaa caggatgatc
     5281 aggcagccga cgattgccac cgtgacgccg tgcccgatgc cccacagcgc accgatcgcg
     5341 gcggcaccgc gcaggctgcg ctcgcgcgtg acgatggtgg cgatggccac cacgtgatcc
     5401 gcgtcggtgg catggcgcat gcccaggaag aatcccaccg cgatgagcga gagcagcgtc
     5461 agcacgtgac ccgtgactcg tgattcgtga ttcgtggatg gtatcagaag tgcactttac
     5521 gaatcacgat gcacgtgctt cactccagcc agcggcgcac ccacggcgcg cggcgcacga
     5581 acaggtcggc gagatacagc gccagcgcga cggcggcgag ccacggccac aacggcgtgc
     5641 gggatcgccc gcggtcgccc tggtcggcga agatctcggt gacgctcgcg ccgaccttgc
     5701 cgccggtctg ctcggcgagc gccgtgagca gctcgaggtc gggcggcagg ctgtgatact
     5761 cgtcgggata cggatagaag agctggcgcg taccggcacg ccgcgcggtc tcgacgccca
     5821 cgccgccgcc gctcaccacg gcgaaggtgt acggccggct gccggcggtg gccaccgcga
     5881 gggtggcgtc gtaatgcccg gggccggtct ggcgcagcac cagatcccgg cggctgccgt
     5941 ccggcccggt cgcgcgcacg cgcggcgcga gcccgttgcg ccagccgccc tgttcggtca
     6001 tcaggcgcag ccgcacgcgc gcctcgtcgc cctcgcgcac cacgcggaac agcacctgct
     6061 cgccggtgtc gcgccgcagg gtatcgcgcg tcagttgcgc ccagaacttg ccgtagcctt
     6121 cccattgcag ccactccgcc gcccagcgat tcttcacgtc cgaggcgaac atcacggaac
     6181 gccccaaccc gtattgccag cgcgcgagca acggggcgtc cgcatcggcc gtgagaaaca
     6241 cctccgcacc gtccttggcc ttggtgctgg cgaaaccctg cagcggcggc gccttggcga
     6301 agtccaggcc gcgcagcgcc tcgatccggc gcttgaccac cgggcggaac ggctcctcca
     6361 tcagcgtggc gcgcgagacg ttctgcgtgt cctgcacgaa gagctgcggg agctgctcca
     6421 ggttctcggc gacgtaggcc cggccgccgc cgaaacccgc gatccgctcc atcagccccg
     6481 gatcgccgct gcgcccgatg gtcaccgtgg acacggtgat cttcgaatcc gtcatgcgct
     6541 tcagcagacg gtcgaagtcg gccggcgcgg tgtcgccgtc ggagatcagg atcacgtgct
     6601 tgcgcttggc cgggtggttc tgcagcatgc gccaggcgct ggcgagcgcc gggtagatgt
     6661 tggtctgccc gctcgcctgg atgcggtcga gcagctcctc cgcgcgccgc ttgctgcgca
     6721 cgtactgcag cggcaccgcc tcgtgcggct gcgaatcgaa ggcgaccacg ccgaagtagt
     6781 gctgctcctc gagcagctcg agggtggcac gcgcgccggc cttggccagc gccagcggcc
     6841 ggcccttcat gctgtaggag cggtccaggc acagcaccag ggcgaggtcc ttgcgctttt
     6901 cgcgcgactt gaactcgacc ggcagcacgc gctccagcgc cgtgtcggcg aagccgtcct
     6961 tgccgtaggt gctgtcgccc gcggcgaaga tcaggccgcc gccgctttcg cgcacgtagc
     7021 gctgcagcgc ccgcatgcgc gcggcatcga tccggtcggg cgcgacatcg ctcaggatca
     7081 ccgcgtcgaa gcgctccagc gcatccagtt cgccgggcag ctcctccgcg cccaccgccc
     7141 gcacctcgag gccctcgcgg cggagcgcgg ccgacaggta ctgggccgcc tccggctggc
     7201 cctcgacgta cagcacgcgc ggccgcgggc cgatccacgc cgattgctgc agccggtcgt
     7261 tgtccgccag caggtcgcct tcggcgtgca cggccacccc gatgatcacg ctgccgctgc
     7321 gcgcgatgcg cgtggcgatc gtcacgacgt tgctgcccgc gttcaaccgg aggcgccgcg
     7381 cgccgagcgc gcgcccgtcc gcgctcacgg tgatgcgcgc cggcgtctcc cgttggctca
     7441 ccacgtgcac ggtcagcgag accggttcgt cgcggcgcac gccctcgggc acgtccagcg
     7501 cctcgatcca ggcgtcggac tgcgcctggg gcggcgccgg gaaggcgaac acgcgcaccc
     7561 cgtgggcggt cagccgcggc agagcgcgcc acacgtcccc gcgcgtctgg ttgccgtcgg
     7621 tgaacagcac cacgcgcggc acacgcgcgg gatccagccc gagcagcgcc tggtccagcg
     7681 ccagctccag attggtcgcg ccctggtaaa gcagccggtc gcggtcggcc accgcggcgc
     7741 tgacgccgac cttgaccacg tcctccgcag tgtccaccag gcgcgcgcga tccgcgaaca
     7801 cgaggtagcg cgcggcgccg ggctcggcct gcgcattggc gcgtctcgcg aagtcgagcg
     7861 cggagcgcac gaacccgctc gccacgctgc gcgagacgtc gagcgcatag accaccgaga
     7921 tctcgctgat gcgcgcggtg cgcgccggtt gcatcagcgc cagcaccagg aacaccaggg
     7981 ccagcgtgcg cagcgcggtg gcaaccgcca ggtggccgcg gccgagattg gtgcgcgagc
     8041 gcaggctgac ccaccacgcg agcggcagca gcgccagcag ccacagcggc cagtacgcgg
     8101 cgacggacag cgtgctcata cggtcacccg ccggttgtag gtccaccact cgacggcgag
     8161 caggatcgtg gcaagcgcca gcaacagcat ccaggggtcg atgcgcaggc gcgcggggac
     8221 gggggccggc ggcgccgcag gcgcggagcc ccacgcgaag cggctggcgt tgatggcggt
     8281 cagctgcggg tcgagcgcgt tcacggcgat ccgcacccgc tgctcgcggg tgagcgcggt
     8341 gaacaacccg ggctcggcca cgtcgaacag ggtcgcatcc ggggccgggc gcgtctgcac
     8401 cgcgcgcccc tccaggtcga ggacccgggc ttgcactgac ggcacgctca cctggccgat
     8461 gcggtgggca agcgcccgcg gctcgcgcgt cagccagtcg acggcgttgg acaggaacgc
     8521 gggaaagctc gcctgctgcg cgaagttgga agcctcgagc gcgaagctca acagcgccag
     8581 gcgcgggcct tcgcgggtgg cgaggatcag gggctcgttg cgcgcaccgc gcgccagcgg
     8641 cgcgagcggc agccgttgtg ccgagccggc ttcgtccctg ggcgcgtcga gagccagcag
     8701 cggtgcccgg tccaccagca cgtcgcgcag cgagacgttc gccagcagcg ggtgcgcggc
     8761 atcccatcgc gccagccgga tgtcgctggc atcggtcgcg gagcgcggca gccaggacac
     8821 cgggccgggc gcgatcagca gcgccggcac tgccggcggg cgcggcggcg caaagcggtc
     8881 gagcaccacc gcgtcgaagc gcaccgaatc gcgccagcgt tcgggggtgg ccagcgccac
     8941 ctcgacgcgc ggcagcaggc gcagcgcctg tgccagcgcc gggttgccgg cggtgaacag
     9001 ccccacgcgc acgcggcctt tcccgggcag ataggtgtag gcggtgttgt ccaggtcgag
     9061 cgcgtcggtg tcggtgcgca gcgtggcgcg cagcgggcct tcctcgaaag cggacaagtc
     9121 gagcaccacg tccgcggccg cgccgccgcg cagttgcagc gcacgctcga gcggcgccgc
     9181 cccgacgccc accacctgga cctcgacgcg cttgtcgccg agagacgcat tggtgatctc
     9241 gagataagct tcgtgccggc gcgcgtccgc gggcaacgca cgcacctcga aggcggtgat
     9301 gcccaggttg tcggccatct ggaaggcgga cacgagccgc gcctcgcgcg gcaccgcgaa
     9361 atcggcgacc ccgtcggtga agaaccagag ctgcagccgc tggccggcgc ccgccggccg
     9421 cgccacgtcc gggaaccagg gctgcgcccc ggcgcgcggg gccagcgtgc gcaatcgcgc
     9481 cagcgcatcc tcgcgcgact cgagcgccgg cgtggcgacc tggtgcgtgg tgtcggcaac
     9541 cagatagcgg ctgccggcgc cgctcgcgcg cacgaccgcc tcggcactgt ccagcgcgcg
     9601 cgcgagacgg gttcggccgt cggccccgcg cgccgccatg ctcggaccgg tgtcgatcac
     9661 cagcagcaca tcggccgccg cgccgctcac cgcggcgatc tcggggcggg tgagcgcggc
     9721 ggcgatggcg agcgcgatgc cgagcgccag catcagcgac aaccaccagc gccagcgctc
     9781 ggcgcggcgc ttgcggcttt ccagcacgcg ccgccagatc aagcccgagg acaccaccag
     9841 gcgtcgcgcc gagggcttga gccagtagag caggaccaca agccccgccg tgcccgcgag
     9901 cagcagccag gcgcccgcgg ccggcaaccc gaggaatctc actccatgcg cctcaccgca
     9961 gcaggccttc ccggcgcagc accttcagca ccaggtcctc gaacggcacg tcggtgagcg
    10021 ctcgggcgta gccccagccg tacttgcggc aataggcctc gatgtcgcgc gcgtgctgct
    10081 cgaaggtgct gcggtaggcg ccgagcagcg ccggggtgat ctccacctcg gtggcggtgc
    10141 cctcttcggc gtccaccagc accacctcct cgggcagttg cgggtcgcgt tcgtcgggcg
    10201 ccatcacgtg cagcagcgcc acgtcgtgac gaaagcgacg cagcacagcc agcgcctcct
    10261 cgatgccctc ggggtcgagg aagtccgaga tcaggatcac cagcccgcgc gtgcgcggca
    10321 cggagaagta ccggcgcagc gccgcgtgca aacgcgtgcg cccgcgcggc tgcaacgact
    10381 gcaggaagcg gaacagctgc ggggcctggc tcctgccgcg gcgcgtgggc agcatctcgc
    10441 gcaggccatc ggcgaacgcg ctgaggttga cccggtcgtg gttgagcagc gccacgtagc
    10501 cgagcgcgcc ggcgagcttg cgcgcgaact ccagcttgtt cggcttgccg tggtccatcg
    10561 actggctggc gtcgaggaac acgtacaccg gcaggtcggc ttcttcctcg aacaggcgca
    10621 ggatcagccg gtccaggcgc aggtagatgc cccagtcgat gttgcggatg tcgtcgcctt
    10681 cgctgtaggg acggtagtcg gcgaacacca tgccggtgcc cacgcgccgc gagcggtgca
    10741 tgccgcgcag ctgcccgta
//
LOCUS       DASPGE010000336         6082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_204705, whole genome shotgun sequence.
ACCESSION   DASPGE010000336 DASPGE010000000
VERSION     DASPGE010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204705"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VD791_10830"
     CDS             complement(<1..230)
                     /locus_tag="VD791_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08514.1"
                     /translation="MRDNPVKKKLLAGQPVFGTFGWEFLVPGLPQIVKAAGAEFLLMD
                     MEHSGAGYETIKSQVAECRGIDLVPMARVPTH"
     gene            complement(366..1388)
                     /locus_tag="VD791_10835"
     CDS             complement(366..1388)
                     /locus_tag="VD791_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV08515.1"
                     /translation="MEKFRVGITRDVLSPAGEPVYGRAALKVLDDPRIQWEYLPEAVP
                     VLTPEHASRYDAICMFGSRCARETVSGPGRRLKLIARFGVGYDNVDVPAITEQGIMLT
                     IAPDGVRRPVASSVMAFLLHMAHRLDTKDRLVRDGRWKDKGDFLGYGLTGKKLGVIGV
                     GNIGMEVFRLARPWGMVHLGCDPNVPQAAMSEVGAEKVDLDTLLREADVVSVNCLLNE
                     QTHHLIGARQFALMKPTVFFINTARGPVVDEAALTDALRSRKIAAAAIDVFEQEPTPT
                     DNPLLELDNVLVAPHAVCHTDECMRLLGESAFASAVELANGRRPRHIVNPEALSHPAW
                     GGKWHK"
     gene            complement(1403..2158)
                     /locus_tag="VD791_10840"
     CDS             complement(1403..2158)
                     /locus_tag="VD791_10840"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXV08516.1"
                     /translation="MFSLQGKIALVTGASRGLGWAMAQSLASAGAHVILNARDPAALD
                     ERVEQLRAQGGRGESAAFDVTDHSASAACIQGAVRRHGRFDILVANAGIQHRKPIIAF
                     EAEDFRRIIDTNLTAVWALAKEAARAMIPRQAGRIIMTGSMSVILARPTISAYVASKG
                     GVHALARELAVELAPHKITVNAIAPGYFATEMNTALIENKEFNDWVCKRTPAGRWGTL
                     QEIGPAAVFLASDEASFVNGQVLSVDGAFSASM"
     gene            2328..4394
                     /locus_tag="VD791_10845"
     CDS             2328..4394
                     /locus_tag="VD791_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXV08517.1"
                     /translation="MEQPEQRRLTAIMFTDMVGYSALSQKNEALALELLQEQRTLLRA
                     AFLKHGGREIEAVGDGFFAEFPSALAGARCAVEIQGTLRDRNASTTQDRRILVRIGLH
                     LGDVVVRDAYVHGDGVNIAARIEPLAEPGGISLSEDVARQIQNKIELPLRRLGRAELK
                     NIRMPVDIYRLVLPWEKARSPGAERLAFGLRRREVRRALLGAGALAVVGGLAGTWAWR
                     RAQSPAGRINRRLAVLPFVNLSAAADNEYFADGITEELISRLSRVGGLEVIARTSVAA
                     YKGTQKKIAEIAAELDVGTVLEGSVRKAGDQVRVTAQLIDATNQAHLWSQDYDRELRD
                     IFSVQREIAQHVADALARRLGVSAAAQREAGGTSNLGAYNAYLQGRFHYNKGDMDGLH
                     KAVEYYGEAISQDSSYALAYAALAEVHEQIAAFEADSSRFPEARTAALRALDLDPSVA
                     EAHTALGVVQTFYDWDWSGARESFGRALALNPNSAVAHNWYGWYLLFLREWDHAIAEL
                     RRAVELDPVSIIMNNDLAVGFQLAGRWDESITQSRRTDTLDPGSLWALNAEAWSHVGR
                     GDYQQALPLFQKQADLAGRDMFTLNALAATYAFSGDTQQALRLLDEMKEKFAGKPGAA
                     YQIQVVYWALAARDERYRTEMYRWLDKACEERSFHLVNTSGRWYDGYQSDPRWVAFRK
                     KLGLPP"
     gene            complement(4407..5381)
                     /locus_tag="VD791_10850"
     CDS             complement(4407..5381)
                     /locus_tag="VD791_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HXV08518.1"
                     /translation="MSKAIRFHRTGGPEVLQLDEVSVGDPGPNQARVRNTAIGLNYID
                     TYHRSGLYPLPLPSGIGQEACAVVEAVGPGVTWVKPGDRVGYAAGPPGAYSEVRIMAA
                     DRLVKVPDGMSDQQVAAMMLKGMTVQYLIRRTYRVEKGETVLFHAAAGGVGLIACQWL
                     KALGATVIGTVGSDEKARIAKAHGCDHTIVYTRENFVERVKEITGGQKVPVVYDSVGK
                     DTFMGSLDCLKPRGLLALFGNGSGPVAAFDVNLLAAKGSLYVTRPSLVNYTARREDLE
                     ATAKDLFAVVQSGKVKIEINQTYALKDAVQAHRDLQARKTTGSTVLLP"
     gene            complement(5460..5798)
                     /gene="soxZ"
                     /locus_tag="VD791_10855"
     CDS             complement(5460..5798)
                     /gene="soxZ"
                     /locus_tag="VD791_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HXV08519.1"
                     /translation="MSQEAGAARVRVPERAKRGEVIEIRAMIEHPMESGFRLNNVGKP
                     IPRHIVESFTCEYDGREIFRARLHPAVSTNPYFLFYTVATASGELRFTWKDDHGGVTT
                     HIARIEVSAQ"
     gene            complement(5795..>6082)
                     /locus_tag="VD791_10860"
     CDS             complement(5795..>6082)
                     /locus_tag="VD791_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HXV08520.1"
                     /translation="LPKLAENGAVVPVTVSVDSPMTERDHVKSIHLFAEKNPLPRILE
                     VQLGPNSGKARVASRIRIADSQQITAVAVLSDGTLWSAAVQVEVTVTGCGL"
BASE COUNT          931 a         2081 c         2069 g         1001 t
ORIGIN      
        1 tgatgcgtgg gcacacgcgc catcggcacg aggtcgatgc cgcggcactc ggcgacttgg
       61 ctcttgatgg tttcgtagcc ggcgccgctg tgctccatgt ccatcagcag gaactcggcg
      121 cccgcggcct tgacgatctg cggcaggccg ggcaccagga actcccagcc gaacgtgccg
      181 aacaccggct ggccggcgag gagcttcttc ttgacggggt tgtcgcgcat ggttggtcct
      241 tctgcaatcg gcttctaccg aagagcgcta tcgaaccgca gtggacgccg aggacgcgga
      301 ggaaaaccct aacaattctc agccctccgc gccctctgcg tcctccgcgg tgagtatcgc
      361 atttctcact tgtgccactt cccgccccag gcgggatggc tcagcgcttc cggattcacg
      421 atgtggcgcg ggcggcggcc gttggccagc tccaccgccg aggcgaacgc actctcaccc
      481 agcaggcgca tgcactcgtc ggtgtggcac accgcgtgcg gcgccacgag tacgttgtcc
      541 agctcgagca gcgggttgtc cgtcggcgtg ggctcctgct cgaacacgtc gatggcggcg
      601 gccgcgatct tgcggctgcg cagcgcatcg gtgagcgccg cttcgtccac caccggcccg
      661 cgcgcggtat tgatgaagaa cacggtgggc ttcatcagcg cgaactggcg cgcgccgatc
      721 aggtgatggg tctgttcgtt gagcaaacag ttgaccgaca cgacgtcggc ctcccgcagc
      781 agcgtgtcga ggtcgacctt ctccgcgccg acctcgctca tcgccgcctg cggcacgttc
      841 gggtcgcagc ccaggtgcac catgccccag gggcgcgcca ggcggaacac ctccatgccg
      901 atgttgccca cgccgatcac gccgagcttc ttgccggtca agccgtaccc gaggaaatcg
      961 cccttgtcct tccagcgtcc gtcgcgcacc agccggtcct tggtgtcgag ccggtgcgcc
     1021 atgtgcagca ggaacgccat cacgctgctc gccaccggcc ggcgcacgcc gtcgggcgcg
     1081 atggtgagca tgatcccctg ctcggtgatc gccggcacgt ccacgttgtc gtagcccacg
     1141 ccgaagcgcg cgatcagctt caggcgtcgt ccggggccgc tcaccgtctc gcgggcgcag
     1201 cgcgagccga acatgcagat cgcgtcgtag cggctggcgt gctcgggcgt gagcaccggc
     1261 accgcctcgg gcaggtactc ccactggatg cgcggatcgt ccagcacctt gagcgccgca
     1321 cgcccgtaca ccggctcgcc ggccgggctc agcacgtcgc gggtgatgcc tacgcggaac
     1381 ttttccatat cgtctccttg cctcacatcg acgcgctgaa ggcgccgtcc accgacagca
     1441 cctgcccgtt gacgaacgac gcctcgtccg aggcgaggaa caccgccgcc ggcccgatct
     1501 cctgcagcgt gccccagcgc cccgcggggg tgcgcttgca cacccagtcg ttgaactcct
     1561 tgttctcgat cagcgccgtg ttcatctcgg tggcgaagta gccgggcgcg atggcgttca
     1621 ccgtgatctt gtgcggcgcc agctccaccg ccagctcgcg cgcgagcgcg tgcacaccgc
     1681 ccttgctggc gacataggcc gagatggtgg gccgggcgag gatcacgctc atcgagccgg
     1741 tcatgatgat gcggccggcc tggcgcggga tcatcgcgcg cgcggcctct ttcgccagcg
     1801 cccacacggc ggtgagattg gtgtcgatga tgcgccggaa atcctcggcc tcgaacgcga
     1861 tgatcggctt gcggtgctgg atgccggcgt tggccaccag gatgtcgaaa cggccgtgtc
     1921 gccgcaccgc accctggatg caggccgcgc ttgcgctgtg gtcggtgacg tcgaaggcgg
     1981 cgctttcgcc gcggccaccc tgtgcgcgca gctgctcgac gcgctcatcc agggccgcgg
     2041 ggtccctggc gttcaagatg acgtgcgcgc cggcgctggc cagcgactgc gccatcgccc
     2101 agcccagacc gcgcgatgcg ccggtgacca gcgcgatctt gccttgcagc gaaaacatct
     2161 cctcccctct gctccgtgga acagggcaat gataaggcat gcgggcgccg gccgccccgg
     2221 tgcggaggat gcggccttga caagaggagg caacccgcca caatgagggc ccgccgagcg
     2281 gcggatccga tgatccccgg atccgatgag ccccggatct gatcaccatg gagcaaccgg
     2341 agcagcgacg cctcaccgcc atcatgttca ccgacatggt gggatacagc gcgctgtcgc
     2401 agaagaacga ggcgctggcg ctcgagctgc tgcaggagca gcgcaccctt ctgcgagcgg
     2461 cgtttctcaa gcacggcggc cgcgagatcg aggcggtcgg cgacggcttc ttcgccgagt
     2521 tcccgagcgc gctggcgggt gcgcgctgcg cagtcgagat ccagggcacg ctgcgcgacc
     2581 gcaacgcctc caccacgcag gaccgacgca tcctggtacg catcggcctg cacctgggcg
     2641 acgtggtggt ccgcgatgct tacgtccacg gcgacggggt caacatcgcc gcgcgcatcg
     2701 agccgctggc cgaacccggc gggatcagcc tgtcggagga cgtggcgcgc cagatccaga
     2761 acaagatcga gctgccgctg cgcaggctcg gcagggcgga gctgaagaac atccgcatgc
     2821 cggtggacat ctaccggctg gtgctgccct gggagaaggc gcgctcgccg ggcgccgagc
     2881 ggctggcgtt cggcctgcgc cggcgcgagg tgcggcgtgc gctgctcggc gccggcgcgc
     2941 tggcggtcgt cggcgggctg gccggcacct gggcctggag acgcgctcag tcgcctgcag
     3001 ggcggatcaa cagacgcctc gccgtgctgc ctttcgtcaa cctgagcgcg gcggccgaca
     3061 acgagtattt cgccgatggg atcacggagg aattgatctc gcggctgtcg cgcgtcggcg
     3121 gcctggaggt gatcgcccgc acttcggtgg cagcctacaa gggaacccag aagaagatcg
     3181 ccgagatcgc cgccgagctc gatgtgggca cggtgctcga ggggagcgtg cgcaaggccg
     3241 gcgaccaggt gcgggtgact gcgcagctca tcgacgcgac caaccaggcg catctatggt
     3301 cgcaggatta cgaccgcgag ctgcgggaca ttttctcggt gcagcgggag atcgcccagc
     3361 atgtcgccga cgcgctggcg cgtcggttgg gcgtctcggc ggcggcgcag cgcgaagcgg
     3421 gcggcacgtc gaatctcggc gcgtacaacg cctaccttca ggggcgtttc cattacaaca
     3481 agggcgacat ggacggtttg cacaaggccg tcgagtacta cggggaggcg atctcgcaag
     3541 actcatccta cgcgctggcg tacgccgcgc tcgccgaggt gcacgagcag atcgcagcct
     3601 tcgaagccga ttcgtcgagg ttccccgagg cgcgaaccgc cgcgttgcgt gccttggatc
     3661 tcgacccgtc ggtcgccgaa gcgcacaccg cgctcggggt ggtacagacg ttctacgatt
     3721 gggactggag cggcgcccgc gaatctttcg ggcgcgcgct ggcgctcaac ccgaactccg
     3781 ccgtggcgca caactggtac ggctggtacc tcctgtttct gcgcgagtgg gatcatgcca
     3841 tcgccgagct gcggcgcgcc gtcgagctgg atccggtctc catcatcatg aacaacgatc
     3901 tggccgtggg tttccagctt gccggccgct gggacgaatc gattacccag tcgcgccgga
     3961 cggacacgct ggatccgggc agtctgtggg cgctgaacgc cgaggcctgg agtcacgtgg
     4021 gcagagggga ctaccagcag gccctgccgc tgtttcagaa gcaggcggac ctcgcgggca
     4081 gggacatgtt cacgctcaac gctctcgcgg ccacctacgc gttttccggc gacacacagc
     4141 aggcgcttcg gctgctggac gagatgaagg aaaaattcgc gggcaagccc ggcgcggcct
     4201 accagataca ggtcgtctat tgggcgctgg cggcgcgcga cgaacgctat cgcacggaga
     4261 tgtatcgctg gctggacaaa gcctgcgagg agcggtcgtt ccacctcgtc aacaccagcg
     4321 ggcgctggta tgacggttac caatccgacc cgcgctgggt cgccttccgc aagaagctcg
     4381 gcctgcctcc gtgagcggca ctcgcctcag ggcagcagca cggtcgagcc ggtggtcttg
     4441 cgggcctgca gatcgcggtg cgcctgcacc gcgtccttca gcgcgtaggt ctggttgatc
     4501 tcgatcttca ccttgccgga ctgcaccacc gcgaacaggt ccttggccgt ggcttcgagg
     4561 tcctcgcgcc gggcggtata gttcaccagg ctcggccgcg tgacgtagag cgagcccttc
     4621 gccgccagca ggttgacgtc gaacgccgcc accggcccgg aaccgttgcc gaacagggcc
     4681 agcagcccgc gcggcttcag gcagtcgagc gagcccatga aggtgtcctt gcccaccgag
     4741 tcgtacacca ccggcacctt ctggccgccg gtgatctcct tcacgcgctc gacgaagttc
     4801 tcgcgcgtgt acacgatggt gtggtcgcag ccgtgcgcct tcgcgatcct ggccttctcg
     4861 tccgagccga ccgtcccgat cacggtcgcg cccagggcct tcagccactg gcaggcgatc
     4921 aggccgacgc cgccggcggc agcgtggaac agcacggtct cgcccttttc cacccggtag
     4981 gtgcggcgga tgaggtattg caccgtcatg cccttcagca tcatggcggc gacctgctgg
     5041 tcgctcatgc cgtcagggac tttcaccagg cgatcggcgg ccatgatccg cacctcggag
     5101 tacgccccgg gcgggccggc ggcgtacccg acgcggtcgc cgggcttgac ccaggtgacc
     5161 cccgggccca ccgcctcgac cacggcgcaa gcctcctgcc cgatgccgct cggcagcggc
     5221 agcggataca ggccgctgcg gtgataggtg tcgatgtagt tgaggccgat ggcggtgttg
     5281 cgcacccgcg cctggttggg cccggggtcg ccgacgctca cctcgtccag ctgcagcact
     5341 tccggtccgc cggtgcggtg gaagcggatc gccttgctca tggtctcccc ctgtggtttg
     5401 gccgctattg tagcccggat acagcgcagc ggaatccggg gaaaagggaa gcgggaagac
     5461 tactgcgcgc tgacttcgat gcgggcgata tgcgtggtca cgccaccgtg gtcgtccttc
     5521 cacgtgaagc gcagctcgcc gctggcggtg gccacggtgt agaagaggaa gtacgggttg
     5581 gtggacacgg cggggtgcag ccgcgcgcgg aagatctcgc ggccgtcgta ctcgcaggtg
     5641 aagctctcga cgatgtggcg cgggatcggt ttgccgacgt tgttcagccg gaagcccgat
     5701 tccatcgggt gctcgatcat cgcgcggatc tcgatcactt cgccgcgctt ggcgcgttcg
     5761 ggcacgcgta cgcgcgcggc gcctgcttcc tggctcacag cccgcacccc gtcacggtca
     5821 cctccacctg caccgccgcc gaccacagcg tgccgtcgct cagcaccgcg accgcggtga
     5881 tctgctgcga gtcggcgatg cggatgcggg aggccacgcg cgccttgccg ctgttcgggc
     5941 cgagctgcac ttcgaggatg cgcggcagcg gattcttctc cgcgaacaga tggatgctct
     6001 tgacatggtc gcgctcggtc atcgggctgt ccaccgacac cgtgaccggc acgaccgcgc
     6061 cgttctcggc gagcttcggc ag
//
LOCUS       DASPGE010000337         7159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1727842, whole genome shotgun sequence.
ACCESSION   DASPGE010000337 DASPGE010000000
VERSION     DASPGE010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727842"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="VD791_10865"
     CDS             <1..140
                     /locus_tag="VD791_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HXV08521.1"
                     /translation="LRSRDPRSGIRDFEAVDALATKQGLILERDVAMPANNRTLVWRR
                     R"
     gene            310..1164
                     /locus_tag="VD791_10870"
     CDS             310..1164
                     /locus_tag="VD791_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HXV08522.1"
                     /translation="MDFPVLTEPATGGGWRARLDLSFAQRGAGTVLARRSHRGPLVVQ
                     KPLYQEGREVCQAIVVHPPGGIAGGDALSLGIEVGAAAHALITTPGASKWYRSAGPEA
                     TQSARLTVARGGVLEWLPQETIVFDGARARLRTEVELEREARFIGCEVVCFGRIASGE
                     RFRSGLLAQACEIRHDGAPMFGEYAQVEGGSPMLEARSGLAGHPVSGVMLAAGCDLDR
                     ATRDRLRALDPGGDALAGVTALPGVTILRYLGGSAQAARAYFVDAWALLRPALTGRDA
                     VPPRIWSC"
     gene            1173..1475
                     /gene="ureA"
                     /locus_tag="VD791_10875"
     CDS             1173..1475
                     /gene="ureA"
                     /locus_tag="VD791_10875"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HXV08523.1"
                     /translation="MELTPREKDKLLIFTAALLAERRKARGLKLNYPEAVAYLSAAIM
                     EGARDGRSVAELMSYGATLLARADVMEGVAEMIPEIQVEATFPDGTKLVTVHHPIS"
     gene            1497..1802
                     /locus_tag="VD791_10880"
     CDS             1497..1802
                     /locus_tag="VD791_10880"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HXV08524.1"
                     /translation="MIPGEIKTQPGEIELNAGRKTVTLTVANTGDRPIQVGSHYHFYE
                     TNEALRFERRQAYGFRLDIPAGTAVRFEPGQERSVRLVELAGERAVYGFLGKVMGKL"
     gene            1802..3508
                     /gene="ureC"
                     /locus_tag="VD791_10885"
     CDS             1802..3508
                     /gene="ureC"
                     /locus_tag="VD791_10885"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HXV08525.1"
                     /translation="MAGKMTRSAYAEMFGPTVGDRVRLADTELWIEVERDHTVYGEEV
                     KFGGGKVIRDGMGQSQRRAAEVADTVITNALIVDYWGVVKADIGIKNGRIGAIGKAGN
                     PDIQPGVDIVIGAATEIIAGEGLIATAGGLDTHIHFICPQQIEEALMSGVTTMIGGGT
                     GPATGTNATTCTPGPWHIHRMLQAADAFPVNLGFLGKGNASLRNPLREQIAAGAIGLK
                     LHEDWGTTPAAIDNCLAVAEEMDVQVAIHTDTLNESGFVETTLAAFKGRTIHTYHTEG
                     AGGGHAPDIIRACGELNVLPSSTNPTRPYTVNTIDEHLDMLMVCHHLDPAIAEDVAFA
                     ESRIRRETIAAEDVLHDLGAFSMMSSDSQAMGRVGEVILRTWQTAHKMRVQRGALPQD
                     KAGNDNFRVKRYVAKYTINPALTHGIAHEVGSLEAGKLADIVLWRPAFFGVKPSLVLK
                     GGMIAAAAMGDPNASIPTPQPVHYRNMFGSFGSACHASCVTFVSAAGLEQSVGQHLGL
                     RKRLVPVRGTRRLTKKHLKLNDATPRIEVDPETYAVRADGELLTCEPATVLPMAQRYF
                     LF"
     gene            3519..4199
                     /locus_tag="VD791_10890"
     CDS             3519..4199
                     /locus_tag="VD791_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HXV08526.1"
                     /translation="MHAHALTRLLRLSSPALPVGAYSYSQGLEAAIGLGWLRGEADIG
                     RWVQDVLAFNLARLEGPVLCRLYRAWERRDTDAAIEWNARFLAARETAELYAETRQMG
                     GSLRGLLRATGELDGRSLASLERVPSPAFPTVFAFAAVSWAIPLPATLTGYFFAWAEN
                     QVGAAMKTMQLGHIGAQRILAGVSAALPALVESAQACDDRGFSNFTPALAIAGARHET
                     QDGRLFRS"
     gene            4213..4659
                     /gene="ureE"
                     /locus_tag="VD791_10895"
     CDS             4213..4659
                     /gene="ureE"
                     /locus_tag="VD791_10895"
                     /inference="COORDINATES: protein motif:HMM:NF009752.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HXV08527.1"
                     /translation="MLKIDHIPGFATDPAIAEALHDLEHRGAVEYLVLSPADTQRHRL
                     RALTDRGTECAIALPRAQQLANGAVLWLERGRAIVVRMSEVEWLTLEPRDAAAALELG
                     YCAGNMHWKVEFAGARLRIALQGPADSYLERLTHLTRDGRVAVQQA"
     gene            4656..5303
                     /gene="ureG"
                     /locus_tag="VD791_10900"
     CDS             4656..5303
                     /gene="ureG"
                     /locus_tag="VD791_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HXV08528.1"
                     /translation="MSSVARAPARGAHPGAARVGIGGPVGSGKTALLERLIPRFRARG
                     TNLAVITNDLVTREDAERVRRSGLIDPARVLAVETGACPHTAIREDPTLNLQAADALE
                     AQFGPLDLILIESGGDNLASTFSLDLVDYWLFVIDTAGGDDIPRKKGLGIVQCDLLVI
                     NKVDLAPYVRVDVERMVREADQVRQGRPILLTNCHSGEGVDAVVERIAADVLFDR"
     gene            complement(5380..6489)
                     /locus_tag="VD791_10905"
     CDS             complement(5380..6489)
                     /locus_tag="VD791_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXV08529.1"
                     /translation="MKITDVTLTLFAWDDIPPTTYGTHTGRFAGSSALGLLRLTTDQG
                     LEGHAFLGSAMNPATSDGQALVTHLKPVLLGKNPLDRERLNQQLWMRQRGAGVRPIGA
                     VDVALWDLAGKAYGQPIHRLLGTYRESIAAYASSAVYGSPEAYAEEALAFKANGWAAY
                     KIHPPTRWREDIAVCEAVRRAVGEAYTIMLDSTWSYDFPAAVRVGRAIEEMGFHWYED
                     PLADQDLYNYVKLKQKLHIPVMATEYPITGYDSYAPWIMLQATDYLRGDVAVKGGITT
                     LVKTAHLAEAFRMNYEIHHGGNSLNNVANLHVIMAIRNTEFFEVLLPDGAQKYGLAED
                     ITVDRDGLVHAPSAPGLGHEIDFALIERKKISTLR"
     gene            6654..>7159
                     /locus_tag="VD791_10910"
     CDS             6654..>7159
                     /locus_tag="VD791_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXV08530.1"
                     /translation="MDRHPADDLISQYLILQQPYYRPTGDEIALYEAAYAVRMPMMLK
                     GPTGCGKTRFVECMAWRLGRPLVTIACHEDMSASDLVGRYLLDAAGTRWQDGPLTLAV
                     RHGAICYLDEVVEARQDTTVVIHPLTDARRVLPLEKRGELVHAHPDFQLVISYNPGYQ
                     NIMKDLKQS"
BASE COUNT         1099 a         2470 c         2497 g         1093 t
ORIGIN      
        1 cgctgcgcag ccgggatccg cgcagcggca tccgcgactt cgaagcggtc gatgcgctgg
       61 cgacaaagca ggggctcatc ctggaacgcg acgtcgccat gcccgccaac aaccgcactc
      121 tggtatggcg gcgccgctag agactgtcca cacacgcacg gatccggggc gccgccgtac
      181 tacgccgcac tactctgggg cgtgatttcg cctcgcgtgc gtggtagcct tgcgtggttg
      241 ccgttgcacg cgcagcacac gctgcgtttg caggcaggtc cgcgcatgcg cgcgtcatcc
      301 tgaatcttca tggacttccc cgttctgaca gaacccgcga ccggcggcgg ctggcgcgcg
      361 cgtctcgatc tgtccttcgc gcagcgcggc gctggcacgg tcctggcaag gcgcagccat
      421 cgcggcccgc tggtggtgca gaagccgctc taccaggaag ggcgcgaggt gtgccaggcc
      481 atcgtcgtgc atccgccggg cggcatcgcc ggcggcgatg cgctgtcgct gggcatcgag
      541 gtcggggccg ccgcgcatgc gctgatcacc acgccgggcg cgtccaagtg gtaccgctcg
      601 gcgggacccg aggccacgca gagcgcgcgg ctgacggtag cgcgcggcgg cgtgctggag
      661 tggcttccgc aggaaacgat cgtgttcgac ggcgcgcgtg cgcggctgcg caccgaggtg
      721 gagctggagc gggaagcccg cttcatcggc tgcgaggtcg tgtgcttcgg gcgcatcgct
      781 tcgggagagc gctttcgcag cggcctgctg gcgcaggcgt gcgaaatccg gcacgacggt
      841 gcgccgatgt tcggcgagta cgcgcaagtg gagggcggca gcccgatgtt ggaagcgcgc
      901 agcggactcg ccggccatcc ggtgagcggc gtgatgctgg cggccggatg cgacctggac
      961 cgcgcgacgc gtgaccggtt gcgtgccctg gaccccggcg gcgatgcact cgccggggtg
     1021 accgcgctgc ccggcgtcac tatcctgcgc tatctgggcg gctcggcgca ggcggcgcgc
     1081 gcgtacttcg tcgacgcctg ggcgctgctg cgcccggcgc tgaccggccg ggacgcggtg
     1141 ccgccgcgca tctggagctg ctgagtcgga cgatggagct taccccgcga gagaaggaca
     1201 agctgctgat cttcaccgcg gcgctgctcg ccgagcggcg caaggcgcgc gggctgaagc
     1261 tcaactaccc ggaagcggtg gcctacctga gcgcggccat catggagggt gcgcgcgacg
     1321 ggcgcagcgt ggccgagctg atgagctacg gcgcgacgct tctcgcgcgc gcggacgtga
     1381 tggaaggcgt ggcggagatg atccccgaga tccaggtgga ggcgactttt cccgacggca
     1441 ccaagctggt cacggtgcac catcccatat cctgatgcta cacgaatcac gcggccatga
     1501 ttccaggtga gatcaagacg caacccggcg agatcgagct caacgccggc cgcaagacgg
     1561 tgacgctgac ggtcgccaac accggcgacc ggccgatcca ggtgggctcg cactaccact
     1621 tctacgagac caacgaggcg ctgcgcttcg agcgccggca ggcctacggc ttccggctgg
     1681 atatcccggc gggcaccgcg gtgcggttcg agccgggcca ggagcgcagc gtgcggctgg
     1741 tcgagctggc cggcgagcgc gcggtgtacg gtttcctggg caaggtgatg gggaagctct
     1801 gatggccggc aagatgacgc gctcggccta cgccgagatg ttcggcccga ccgtcggcga
     1861 ccgcgtgcgg ctggccgaca ccgagctgtg gatcgaggtc gagagggacc acaccgtcta
     1921 cggcgaggag gtgaagttcg gcggcggcaa ggtgatccgc gacggcatgg gccagagcca
     1981 gcgccgcgcc gccgaggtgg ccgacacggt gatcaccaac gccctgatcg tggactactg
     2041 gggggtggtc aaggccgaca tcggcatcaa gaacgggcgc atcggcgcca tcggcaaggc
     2101 cggcaatccg gacatccagc ccggtgtgga catcgtcatc ggggcggcca ccgagatcat
     2161 cgcgggcgag ggcctgatcg ccaccgccgg cggcctggac acgcatatcc acttcatctg
     2221 cccgcagcag atcgaggagg cgctcatgtc cggcgtcacc accatgatcg gcggcggcac
     2281 cggcccggcc accggcacca acgccaccac ctgcacgccg gggccgtggc acatccaccg
     2341 catgctgcag gccgccgacg ccttcccggt gaacctgggc tttctcggca agggcaacgc
     2401 cagcctgcgc aacccgctgc gcgagcagat cgccgccggc gcgatcggcc tgaagctgca
     2461 cgaggactgg ggcaccacgc cggcggcgat cgacaactgc ctggcggtgg ccgaggagat
     2521 ggacgtgcag gtggcgatcc acaccgatac gctgaacgag tcgggcttcg tggaaaccac
     2581 gctggccgcg ttcaagggcc gcaccatcca cacctatcac accgaaggcg cgggcggcgg
     2641 tcacgcgccg gacatcatcc gcgcctgcgg cgagcttaac gtgctgccct cgtccaccaa
     2701 tcccacgcgc ccgtacacgg tgaacaccat cgacgagcat ctcgacatgc tcatggtatg
     2761 ccaccatctg gacccggcca tcgccgagga cgtggcgttc gccgaatcgc gcatccgccg
     2821 cgagacgatc gccgccgagg atgtgctgca cgacctgggg gcgttcagca tgatgtcgtc
     2881 cgactcgcaa gccatgggcc gcgtgggcga ggtgatcctg cgcacctggc agaccgcgca
     2941 caagatgcgc gtgcagcgcg gcgcgctgcc gcaggacaag gcgggcaacg acaacttccg
     3001 cgtcaagcgc tacgtcgcca agtacaccat caatccggcg ctcacccacg gcatcgcgca
     3061 cgaggtcggc tcgctcgagg ccggcaagct cgccgacatc gtgctgtggc ggccggcttt
     3121 cttcggcgtc aagccctcgc tcgtgctcaa gggcggcatg atcgcagcgg cggccatggg
     3181 cgatcccaac gcttccatcc ccacgccgca acccgtgcac taccgcaaca tgttcggcag
     3241 cttcggctcg gcctgccatg cgagctgcgt gacgttcgtg tccgccgccg gtctggagca
     3301 gtccgtcggg cagcatctgg gcctgcgcaa gcgcctggtg ccggtacgcg ggacccggcg
     3361 cctgaccaag aagcacctga agctcaacga cgccaccccg cgcatcgaag tcgatccgga
     3421 aacctacgcg gtgcgcgccg acggcgagct gctcacctgc gagcccgcca cggtgctgcc
     3481 gatggcgcag cgttatttcc tgttctgaac gcgggccgat gcacgcgcac gccctgacgc
     3541 gcctgctgcg cctgtcgagc ccggcgctgc cggtgggcgc ctacagctac tcccagggcc
     3601 tggaggcggc gatcggcctg ggctggctgc gcggcgaggc cgacatcggc cgctgggtgc
     3661 aggacgtgct cgccttcaac ctcgcgcgcc tggagggccc ggtgctgtgc cggctgtatc
     3721 gcgcctggga gcggcgggac accgacgcgg cgatcgagtg gaacgcccgt ttcctggcgg
     3781 cgcgcgagac cgccgagctg tacgccgaaa cccgccagat gggcggctcg ctgcgcggac
     3841 tgctgcgcgc caccggcgag ctggacggac gctcgcttgc atccctcgag cgagttccat
     3901 cgccggcctt ccccactgtg ttcgccttcg ccgcggtgtc gtgggcgatt cccctgccgg
     3961 ccacgctgac cgggtatttc ttcgcctggg ccgagaacca ggtcggcgcc gcgatgaaga
     4021 ccatgcagct cggccacatc ggcgcgcagc gcatcctcgc cggcgtgtcc gcggcgctgc
     4081 cggcgctggt cgaatcggcg caggcgtgcg acgatcgcgg cttcagcaat ttcacgccgg
     4141 cgctcgccat cgccggcgcc cggcacgaga cgcaggacgg ccggctattc cgctcctgag
     4201 gggatccatc gcatgctgaa gatcgatcac atcccggggt tcgccaccga cccggccatc
     4261 gccgaagcgc tgcacgatct tgaacaccgt ggcgcggtcg agtacctggt tctgtcgccg
     4321 gccgacacgc aacgccaccg cctgcgcgcg ctgaccgacc gcggcaccga gtgtgcgatc
     4381 gcattgccgc gtgcgcagca attggccaac ggcgccgtgc tgtggctgga acgcggccgc
     4441 gcgatcgtcg tgcgcatgag cgaggtcgag tggctgacgc tggagccgcg cgatgcggcc
     4501 gccgccctcg agctgggcta ttgcgcgggc aacatgcatt ggaaggtgga gttcgccggc
     4561 gcccggctgc gcatcgcgct gcagggcccg gccgacagct acctggagcg cctcacccat
     4621 ctgacgcggg acgggcgcgt ggccgtgcag caagcatgag ctccgttgcg cgagccccgg
     4681 cgcgcggcgc gcaccccggc gccgcgcgcg tgggcatcgg cggtcccgtg ggttcgggca
     4741 agacggcgct gctggagcgg ctcatcccgc gcttccgggc gcgcggcacc aacctcgccg
     4801 tcatcaccaa cgacctggtt acgcgggagg atgcggagcg cgtgcggcgc agcggcctga
     4861 tcgacccggc gcgcgtgctg gcggtggaaa ccggcgcctg cccgcacacc gcgatccgcg
     4921 aggaccccac gctcaacctg caggcggccg atgcgctgga ggcgcagttc gggccgctcg
     4981 acctgatcct gatcgagagc ggcggcgaca acctcgcctc gacgttctcc ctggacctgg
     5041 tcgactactg gctgttcgtg atcgacaccg ccggcggcga cgacatcccg cgcaagaagg
     5101 gcctgggcat cgtgcaatgc gacctgctgg tcatcaacaa ggtcgacctg gcgccctacg
     5161 tgcgggtgga cgtcgagcgc atggtgcgcg aagccgatca ggtgcgccag ggacgcccga
     5221 tcctgctcac caattgccac agcggcgagg gcgtggacgc cgtggtcgag cgcatcgctg
     5281 ccgacgtgct gttcgaccgc tgacccgact ccgcgcgcga agcgcacaag agtcccctct
     5341 ccccttgcgg gctagggtga gggggatgcg cgggatccgt cacctcagcg tcgagatctt
     5401 cttgcgctcg atcagcgcga agtcgatctc gtggcccagg cccggcgcgc tcggtgcgtg
     5461 caccagcccg tcacgatcca cggtgatgtc ctcggcgagg ccgtacttct gggcgccgtc
     5521 gggcaggagc acttcgaaga actccgtgtt tcggatcgcc atgatcacgt gcaggttggc
     5581 cacgttgttg agcgagtttc cgccgtggtg gatctcgtag ttcatgcgga acgcctcggc
     5641 cagatgagcg gtcttcacca gcgtcgtgat gccgcccttc accgccacgt cgccgcgcag
     5701 atagtcggtg gcctgcagca tgatccacgg cgcgtagctg tcgtatccgg tgatcggata
     5761 ctcggtcgcc atcaccggga tgtgcagctt ctgcttgagc ttgacgtagt tgtacaggtc
     5821 ctggtcggcg agcggatcct cgtaccagtg gaaacccatt tcttcgatcg cgcgtccgac
     5881 gcgcacggcc gccggaaaat cgtagctcca ggtcgaatcg agcatgatgg tgtaggcctc
     5941 gcccaccgcg cggcgcaccg cctcgcacac cgcgatgtct tcgcgccacc gggtcggcgg
     6001 atggatcttg taggccgccc agccgttggc cttgaaggcc agcgcttcct cggcgtaggc
     6061 ctcgggcgag ccgtataccg cggacgaggc ataggcggcg atgctctcgc ggtacgtccc
     6121 gagcaggcga tggatcggct gcccatacgc cttgccggcg agatcccaca gggccacatc
     6181 gacggcgccg atcgggcgca cgccggcccc gcgctgccgc atccaaagct gctgattgag
     6241 ccgctcgcga tcgagcgggt tcttgcccag cagcaccggt ttcaggtgcg tgacgagggc
     6301 ctgcccgtcg ctggtcgcgg gattcatcgc cgagcccaga aaggcgtgtc cctcgagccc
     6361 ttgatcggtg gtcaatcgca ggaggccgag cgcgctgctg cccgcgaagc gcccggtgtg
     6421 cgtcccgtag gtggtgggcg gaatgtcgtc ccaggcgaac agcgtgagcg ttacgtcggt
     6481 gatcttcatg gtttcgtcgg tgcatcggtt cggcgctgcg aatctaatcc aatcgcaagc
     6541 gcttggaaag ggtgcgcgcc gcgcgttcgc cggcggtccg gggctgatcg cccctcccgc
     6601 gcgtggtatg ctcggtccac cgggccgagc gccccggcct tggacgcacg cgcatggacc
     6661 gccatcccgc cgacgatctg atcagtcagt acctgatcct gcagcagccg tactaccgcc
     6721 cgacaggcga cgagatcgcc ctgtacgagg ccgcgtatgc ggtacgcatg ccgatgatgc
     6781 tgaaggggcc caccgggtgc ggcaagacgc gcttcgtgga gtgcatggcc tggcgtctcg
     6841 gccggccgct ggtgaccatc gcttgccacg aggacatgag cgcttccgac ctggtggggc
     6901 gctaccttct ggacgccgcc ggcacgcgct ggcaggacgg tccgctcacg ctggcggtgc
     6961 gccacggcgc catctgctac ctggacgagg tggtcgaggc gcgccaggac accaccgtgg
     7021 taatccaccc gctgaccgat gcccgccgcg tgctgccgct ggagaagcgc ggcgagctgg
     7081 tgcacgcaca tccggacttc cagctcgtga tctcctacaa ccccggctac cagaacatca
     7141 tgaaggatct caagcagtc
//
LOCUS       DASPGE010000338         7711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_439746, whole genome shotgun sequence.
ACCESSION   DASPGE010000338 DASPGE010000000
VERSION     DASPGE010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7711
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439746"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VD791_10915"
     CDS             <1..543
                     /locus_tag="VD791_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HXV08531.1"
                     /translation="ALAEVVLERRARLLRAVDSRAPRQIDAIKCRYHGDYHLGQVLIN
                     RNDFVIADFEGEPGRSLPERRAKHSPLRDVAGMLRSFGYARATALSRATGERPEDLQQ
                     LEPPARQWESEARRAFLRAYQEATAGGVLYRSVEQALSLIALFEIEKASYELRYELAN
                     RPDWVAIPLRGILELSDETA"
     gene            601..1293
                     /locus_tag="VD791_10920"
     CDS             601..1293
                     /locus_tag="VD791_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08532.1"
                     /translation="MERVDMLFEPVRALLLEVGAFLPRLGVAMLVLAGGWLLAKAVRF
                     AVVKGLRAVNFNVLTERAGIDGFLEQGGVRTDTAGILGSLVYWLVILAALVIAFNGLG
                     LTYITDLLGRVLLYVPHVILAVLIIVFGTYFARFAGETVAAYLRSTGAQDAELLGRIA
                     QYAIVVFVVLIALDQLAIGGAIVRQTFLILLAGAVLALALAFGIGGARWAGALLERWW
                     PVRREEHHDDGV"
     gene            1410..3545
                     /gene="glgX"
                     /locus_tag="VD791_10925"
     CDS             1410..3545
                     /gene="glgX"
                     /locus_tag="VD791_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HXV08533.1"
                     /translation="MSKPITAVWPGSPYPRGATWDGEGVNFALFSANADKVELCLFDP
                     SGRRELQRIELTEQTDQLWHCYLPEARPGLLYGYRVHGRYSPEEGHRFNPHKLLIEPY
                     AKHLEGSLRWSDAHFGYRIGHRREDLSFDRRDSAPGMPKCRVVDPAFTWGDDRRPRIP
                     WHDTVIYEVHVRGFTLHHPQVPEALRGSYAGLATAPVIDHFRRLGVTTVELMPAHSFV
                     DDRHLIQRGLRNYWGYNSIGFFAPETRYSSSGRISEFKTMVKTLHSAGIEVILDVVYN
                     HTAEGNHLGPTLCFRGVDNASYYRLTPGNERYYMDFTGTGNTLNLTHPRVLQLIMDSL
                     RYWVLEMHVDGFRFDLASALARELYDVDRLGAFFDVISQDPVLSQVKLIAEPWDVGAG
                     GYQVGNFPAGWAEWNDKYRDTMRAYWKGDGGLIGEFARRLTGSSDLYAGGGRTPHASI
                     NYVTAHDGFTLHDLVSYNEKHNEANQEDNQDGHAHNLSWNCGVEGPSDDPDVLALRAR
                     QKRNLIATLLLSQGVPMLLAGDEIGRTQRGNNNAYCQDNEISWVDWDAARDHEGFLRF
                     VRRMIRIRQEHPVLRRRRFFQGRPIRGGGVKDILWLTPEGREMTDHEWTQDFARSLAV
                     YLAGEALAETDARGRRLQDDNFLLLLNAHHDAIEFLLPGMRAGSHWQVLLDTGFEHGL
                     AVDGRFPTGERYRLAGRALALLVEVTDTA"
     gene            3542..5392
                     /gene="treZ"
                     /locus_tag="VD791_10930"
     CDS             3542..5392
                     /gene="treZ"
                     /locus_tag="VD791_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HXV08534.1"
                     /translation="MKRAHPMPFGAQLLDGGATRFRLWAPSARRVELVRSQSGARTAM
                     LPAGEGWYQVDLLDAGPGTLYRYRIDDRIEVPDPASRCNPQDVHGPSMVVDPTAFDWD
                     DDAWRGLPWERSVIYELHVGAFTPQGSFHAVEGKLAHLVELGVTAIELMPVAECPGAH
                     NWGYDGVLPFAPEARYGTPEELKRLIVAAHRHGLMVFLDVVYNHFGPEGNYLGAYAAE
                     FFTERHRTPWGAAINFDGGGSRTVRAFFLHNALYWLEEYHIDGLRLDAVHAIRDDSTL
                     HVLNEIAEAARAHIGGRRHVHLMLENDANEAHYLGVRPGMPDRFDAQWNDDLHHCLHV
                     VVTGEQDGYYRDYAERPHRLLGRALAQGLAYQGEASDYRHGRARGEGSDHLPSTAFVS
                     FLQNHDQIGNRALGERIGKLARPEALKAALAVLLLAPHIPLLFMGEEWNAPEPFLFFC
                     DFEPALAGSVREGRRNEFSRFARYRDQAARLLIPDPTDPASFERSRLDWRAAEREPHR
                     TWLRLCRQLLAVRHREIAPRLAGARGVGYVAGERGDLQVQWRLGDGSLLQLTSNLTDA
                     PIASAAQPRGRQIYTTHPEFGAALTGSGLAPWTVAWSLDAAAAEGNPADA"
     gene            5439..>7711
                     /gene="treY"
                     /locus_tag="VD791_10935"
     CDS             5439..>7711
                     /gene="treY"
                     /locus_tag="VD791_10935"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HXV08535.1"
                     /translation="MIPRATYRLQLHRAFTLRDATDVVPYLAQLGVSHAYCSPYLRAR
                     TGSTHGYDIIDHNALNPEIGSREDFERFVQTLASHGMGQILDMVPNHVGVMGADNAWW
                     LDLLENGQASAYADFFDIDWHPADASLAGKVLVPVLGSQYGVALEGGEFRLALDAQQG
                     SFSLWYHAHRFPIDPREYPRILGPVLRQVSPEALPQGVHAELENLSRDLGNLPPRDAT
                     SAVERVERDRDARVHKGRLAALLQAHAALAAAVDAALAALNGVPGTPRSFDALHELIE
                     RQAYRLAYWRVASDEINYRRFFDINDLAALRMENETVFDATHRLVLQLLAEGKADGLR
                     IDHPDGLYDPAQYFRRLQQGYAAALGKGAAGIAAPGSADAGAAPAAGGGRDWPLYLVI
                     EKIAAGHERVPESWQVYGTTGYRFANVVNGLFVDGAARSRLERTWRAFTGLPASWEDT
                     VYHCKVLIMRTALASELTVLSNQLLRIARADRRTRDFTFNTLRQALIEVVACLPVYRT
                     YLTQPPSASDRRYVEWAVARAKRSSLAADSSLLDFLRGVLLGEAPAGAAPELREAMRS
                     FAGKLQQFTAPVTAKGVEDTAFYRHLPLASVNEVGGDPHRMGTSVRTFHGASRDRAAK
                     WPHTLLTTTTHDTKRAEDVRLRIDVLSEMPGAWRLALKRWSRLNRRHKHTVGGEQAPS
                     RADEYLLYQTLVGSYPPGDLDDAALAEYRARIEAYMLKAVREAKRYSSWINPDPAYED
                     ALQAFVGALLTRRDSNL"
BASE COUNT         1202 a         2679 c         2653 g         1177 t
ORIGIN      
        1 gcgctcgccg aggttgtgct cgagcgtcgc gcccggttgc tgcgggcggt ggactcccgc
       61 gcaccacggc agatcgacgc catcaagtgc cgctatcacg gcgactacca tctggggcag
      121 gtgctcatca accgcaacga cttcgtcatc gccgacttcg agggcgagcc cggccgctcg
      181 ctgcctgagc gccgcgccaa gcactcgccc ctgcgcgatg tggccggcat gctgcgatca
      241 ttcggctacg cgcgcgcgac cgcgctgtcg cgcgccacgg gcgagcgccc ggaggacctg
      301 cagcaactcg aaccgccggc ccgccagtgg gagagcgagg cccggcgcgc gttcctgcgc
      361 gcctaccagg aggcgaccgc cggcggcgtg ctgtaccgtt cggtcgagca ggcgctgtcg
      421 ctgatcgcgc tgttcgagat cgaaaaggcg tcctacgagc tgcgttacga actggccaac
      481 cgcccggact gggtggcgat ccccctgcgc gggatcctgg agttgagcga tgagacggcc
      541 tagcctggat attcccagcg cgggccgcga ggcggggtcg agaacgacgg aggtatggca
      601 atggaacgcg tagacatgct gttcgagccg gtgcgtgcgt tgctgctgga agtcggcgcc
      661 ttcctgccgc ggctgggcgt tgccatgctg gtgctggcag gcggctggct gctcgccaaa
      721 gcggtgcgct tcgcggtggt gaagggactg cgtgccgtga acttcaatgt gctgaccgag
      781 cgcgccggca tagacgggtt cctggagcag ggcggcgtgc gcaccgatac cgccgggatt
      841 ctcggctccc tggtgtattg gctggtgatc ctcgctgccc tggtgatcgc cttcaacggg
      901 ctggggctca cctacatcac cgacctgctg ggtcgcgttc tgctgtacgt gccgcacgtc
      961 atcctggcgg tgctgatcat cgtgttcgga acctatttcg cccgcttcgc cggggagacc
     1021 gtggcggcct acctgcgcag caccggcgcg caggacgccg agctgctggg ccgcatcgcg
     1081 cagtacgcca tcgtcgtgtt cgtggtgctg atcgccctgg accagctggc tatcggcggc
     1141 gcgatcgtgc gccagacgtt tctgatcctg ctggccggcg cggtgctggc cttggcgctc
     1201 gcgttcggga tcggcggtgc gcgctgggcc ggcgcgctgc tggaacgctg gtggccggtc
     1261 cggcgcgagg agcatcacga tgacggcgtc tgattcgacc gggatgatcg ccacggtccc
     1321 ggccggcgat gctgccggcg ccgtgcaccg gaagctgaga tcgatcgcgg cacacacgct
     1381 cgaccgcgca ggctcgcgct gagcgcggca tgagcaagcc gatcaccgcg gtttggcccg
     1441 gctcgcccta tccgcgcggc gccacgtggg acggcgaggg cgtgaacttc gcgctgttct
     1501 ccgccaacgc cgacaaggtg gagctgtgcc tgttcgatcc ttcgggccgg cgcgagctgc
     1561 agcgcatcga gctcaccgag cagaccgatc agttgtggca ctgctacctg cccgaggcgc
     1621 gccccgggct gctctacggc taccgcgtgc acgggcgcta cagcccggag gaggggcacc
     1681 gcttcaaccc ccacaagctg ctgatcgagc cctacgccaa gcatctggag ggcagcctgc
     1741 gctggagcga tgcgcacttc ggatatcgca tcgggcaccg gcgcgaggac ctgtccttcg
     1801 accgccgcga cagcgcgccg ggcatgccca agtgccgcgt ggtggacccg gcattcacct
     1861 ggggcgatga ccgccgcccg cgcataccct ggcacgacac cgtgatctac gaggtgcacg
     1921 tgcgcggctt caccttgcac cacccgcagg tacccgaggc gctgcgcggc agctatgcgg
     1981 gactggccac cgcgccggtg atcgatcact tccggcgcct gggcgtgacc accgtcgagc
     2041 tgatgccggc gcacagcttc gtggacgacc gccatctgat ccagcgcggg ctgcgcaact
     2101 actggggtta caactcgatc ggcttcttcg ctcccgagac gcgctattcc tcgagcggcc
     2161 gcatcagcga gttcaagact atggtcaaga cgctgcactc ggccgggatc gaggtgatcc
     2221 tggatgtggt ctacaaccat accgcggagg gcaatcacct cggacccacg ctgtgtttcc
     2281 gcggcgtgga caacgcttcc tactaccgct tgacgcccgg caacgaacgc tactacatgg
     2341 acttcaccgg caccggcaac accttgaacc tcacccatcc gcgcgtgctg cagctcatca
     2401 tggactcgct gcgctactgg gtgctggaga tgcacgtgga cggtttccgc ttcgacctgg
     2461 cctcggccct cgcgcgcgag ctgtacgacg tggaccggct gggcgcgttc ttcgacgtga
     2521 tctcgcagga tccggtgcta tcgcaggtga agctgatcgc agagccgtgg gacgtgggcg
     2581 caggcggcta tcaggtaggc aactttcccg ccggctgggc cgagtggaat gacaagtacc
     2641 gcgacaccat gcgcgcgtac tggaagggcg acggcggcct gatcggcgag ttcgcgcggc
     2701 gcctcaccgg cagcagcgac ctctacgccg gcggcggccg cacgccgcat gccagcatca
     2761 attacgtcac cgcgcacgac ggtttcactc tgcacgacct ggtaagctac aacgagaagc
     2821 acaacgaggc caatcaggaa gacaaccagg acgggcacgc ccacaacctg tcatggaact
     2881 gcggggtgga aggaccatcc gacgaccccg acgtcctggc gctgcgcgca cggcagaaac
     2941 gcaacctgat cgccaccctg ttgctgtctc agggcgtgcc gatgctgctg gcgggcgacg
     3001 agatcggccg cacccaacgg ggcaacaaca acgcctattg ccaggacaac gagatttcct
     3061 gggtggattg ggacgcggcc cgcgaccacg aaggctttct gcgcttcgtg cgacgcatga
     3121 ttcgcatccg ccaggaacat ccggtgctgc gccggcggcg ctttttccag gggcggccga
     3181 tccgcggcgg gggggtgaag gatatcctgt ggctcacgcc cgagggccgt gagatgaccg
     3241 accacgaatg gacgcaggac ttcgcgcgca gcctggcggt gtacctcgca ggagaagcgc
     3301 tcgcggagac cgacgcgcgc gggcggcgcc tgcaggacga caacttcctg ctgctgctca
     3361 acgcccacca cgacgccatc gagtttctgc tgccgggaat gcgcgccggc agtcactggc
     3421 aggtgctgct ggacaccggc ttcgagcacg gcctggcggt ggacggacgc ttccccaccg
     3481 gcgagcgtta tcgcctggct ggccgcgcgc tagcgctgct ggtcgaagtc acggatacgg
     3541 catgaagcgc gcccatccga tgccgttcgg ggcgcagctg ctcgacggcg gcgcgacgcg
     3601 cttccggctg tgggcgccgt ctgcgcggcg cgtcgagctg gtgcgctcgc agagcggcgc
     3661 gcgcaccgcc atgctgcccg cgggcgaagg ctggtatcag gtggatctgc tcgacgccgg
     3721 accgggcacg ctctaccgct atcggatcga cgaccgcatc gaagtgccgg acccggcatc
     3781 gcgctgcaac ccgcaggacg tgcacggtcc cagcatggtg gtcgatccca ccgccttcga
     3841 ctgggacgac gacgcctggc gagggctgcc gtgggagcgc agcgtgatct acgagctgca
     3901 cgtcggggcc ttcacgccgc agggcagctt ccacgccgtc gagggcaagc tcgcgcatct
     3961 ggtcgagttg ggcgtgaccg cgatcgagct gatgccggtc gccgaatgcc cgggcgcgca
     4021 caactggggc tacgacgggg tgctgccgtt cgcgccggag gcgcgctacg gcacgcccga
     4081 ggagctcaag cgtctgatcg tggcggcgca ccgtcacggc ctgatggtgt ttctggacgt
     4141 cgtgtacaac cacttcggac ccgagggaaa ttacctcggc gcctacgccg ccgagttctt
     4201 caccgagcgg caccgcacgc cttggggcgc ggcgatcaac ttcgacggcg gcggcagccg
     4261 cacggtgcgc gccttcttcc tgcacaacgc gttgtactgg ctggaggaat accacatcga
     4321 cggcctgcgg ctggacgcgg tgcacgccat ccgcgacgat tcgacgctgc atgtgctcaa
     4381 cgagatcgct gaggccgcgc gcgcacacat cggcgggcgg cgccatgtgc acctgatgct
     4441 ggagaatgat gccaacgagg cacattatct cggcgtgcgc ccggggatgc ccgaccgctt
     4501 cgacgcccag tggaacgacg atctgcacca ctgcctgcac gtggttgtca ccggcgagca
     4561 ggacggctac taccgcgatt acgccgaacg gccgcatcgg ctgctcggcc gtgcactggc
     4621 ccagggcctc gcctatcagg gggaggcgtc cgactatcgc catggccgcg cgcgcggcga
     4681 ggggagcgac cacctgccgt ccaccgcctt cgtgtctttc ctgcagaacc acgaccagat
     4741 cggcaaccgc gcgctgggcg agcgcatcgg caagctcgcc cggcccgagg cgctgaaggc
     4801 cgcgctcgcc gtgctgctgc tggccccgca catcccgctg ctgttcatgg gcgaggagtg
     4861 gaatgcaccc gagccgttcc tgttcttctg cgacttcgag cccgcgctgg cgggcagcgt
     4921 gcgcgagggt cgccgcaacg agttctcgcg cttcgcgcgc taccgcgacc aggccgcacg
     4981 cctgctgatc cccgatccga ccgatcccgc cagcttcgag cgctcgcgcc tggactggcg
     5041 cgcggccgaa cgcgaaccgc atcgcacctg gttgcggctc tgccgccaac tgctcgccgt
     5101 tcgccaccgc gagatcgcgc cgcgcctggc cggcgcgcgc ggcgtcggtt acgtcgcggg
     5161 cgagcgcggg gatctccagg tgcaatggcg gctcggggac ggctcgctcc tgcagctcac
     5221 ctcgaatcta accgatgcgc cgatcgcgag cgcagcacag ccgcgcggac gccagatcta
     5281 caccacgcat cccgagttcg gcgcggcgct caccggcagc ggcctggcgc cgtggaccgt
     5341 ggcgtggtcg ctggacgcag ccgcggccga aggaaacccc gcggatgcct gagtccgatc
     5401 cgccgcggca gacgaagccg aaaccggggc gcggcgcggt gatcccccgc gccacctacc
     5461 ggctgcagct ccaccgtgcg ttcaccttgc gcgatgccac cgacgtcgtc ccctatctgg
     5521 cgcagctcgg agtgagccac gcgtactgct cgccctatct gcgtgcgcgc acgggcagca
     5581 ctcacggcta cgacatcatc gaccacaacg cgctcaaccc cgagatcggc agccgcgagg
     5641 acttcgagcg gttcgtgcag accctcgcgt cgcacggcat gggccagatc ctcgacatgg
     5701 tgcccaatca cgtgggggtg atgggagcgg acaacgcctg gtggctggat ctgctggaaa
     5761 acggccaggc gtccgcgtat gcggactttt tcgacatcga ctggcacccg gcggacgcga
     5821 gcctcgccgg gaaggtgctg gtgccggtgc tcggcagcca gtacggcgtg gcgctggaag
     5881 gcggcgaatt caggctcgcc ctcgacgcgc aacagggcag cttcagcctg tggtatcacg
     5941 cgcatcgctt cccgatcgat ccgcgcgagt acccgcgcat cctcggaccc gtcctgaggc
     6001 aggtatcgcc cgaggcgctg ccgcagggcg tgcacgcgga gctcgaaaac ctgagccggg
     6061 atctcgggaa tctgccgccg cgcgacgcga ccagcgccgt ggagcgcgtc gagcgcgatc
     6121 gcgacgcccg cgtgcacaag ggccggctcg cggcgctgct gcaggcgcac gcggcgctgg
     6181 ccgcagccgt agatgccgcc ctggcagcgc tgaacggcgt gccgggcacg ccgcggagct
     6241 tcgacgcgct gcacgagctg atcgagcgtc aggcctaccg gcttgcgtac tggcgcgtgg
     6301 cttcggacga gatcaactat cgccgcttct tcgacatcaa cgacctcgcc gcgctgcgca
     6361 tggagaacga aacggtgttc gacgccacgc accggctggt gctgcagctg ctcgcggagg
     6421 gcaaggcgga cggactgcgc atcgaccatc ccgacggcct gtatgatccg gcgcagtatt
     6481 ttcggcgcct gcagcagggc tacgctgcag cgctcggcaa gggggcggcc ggcatcgcgg
     6541 cgcccgggtc ggcagacgcc ggcgcggccc ccgccgcggg cgggggccgc gactggccgc
     6601 tgtacctggt gatcgagaag atcgccgcgg gccacgaacg cgtgccggag agctggcagg
     6661 tgtacggcac caccggttac cgcttcgcca acgtggtgaa cggcctgttc gtggacggcg
     6721 cggcacgttc gcggctggag cgcacctggc gcgcattcac cggcctgccg gcgtcgtggg
     6781 aggacaccgt ataccactgc aaggtcctga tcatgcgcac cgcgctcgcc agcgagctga
     6841 ccgtgctcag caaccagctg ctgcgcatcg cgcgcgccga tcggcgcacg cgcgatttca
     6901 ccttcaacac gctgcgccaa gcgctgatcg aagtggtggc atgcctgccg gtgtaccgca
     6961 cctatttgac gcaaccgccg tcggcaagcg accggcgcta cgtcgagtgg gcggtggcgc
     7021 gcgccaagcg cagcagcctc gcggccgaca gcagtctgct cgatttcctg cgcggcgtgc
     7081 tgctgggcga ggcgcccgcc ggcgccgcgc cggagctgcg cgaggcgatg cgcagcttcg
     7141 ccggcaagct gcagcagttc accgcgccgg tcaccgcgaa gggcgtcgag gacaccgcgt
     7201 tctaccgcca cctaccgctc gcgtcggtca acgaggtggg cggcgacccg caccgcatgg
     7261 gcaccagcgt gcgcaccttc catggcgcca gccgggatcg ggccgccaag tggccgcaca
     7321 cgctgctcac caccaccacc cacgatacca agcgcgccga ggacgtacgc ctgcgcatcg
     7381 acgtgctgtc cgagatgccg ggagcgtggc gcctggcgtt gaagcgctgg agccggctca
     7441 accgcaggca caagcacacc gtcggcggcg agcaggcgcc gtcgcgagcc gacgagtatc
     7501 tgctgtacca gacgctggtc ggcagctatc ccccgggcga tctcgacgac gccgcgctcg
     7561 ccgaataccg cgcgcgcatc gaggcctaca tgctcaaggc ggtgcgcgag gcgaaacggt
     7621 attcgagctg gatcaatcca gacccggcgt acgaggacgc gctccaggcc ttcgtcggtg
     7681 cgctgctcac ccggcgcgac agcaatctat t
//
LOCUS       DASPGE010000339         5047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1258756, whole genome shotgun sequence.
ACCESSION   DASPGE010000339 DASPGE010000000
VERSION     DASPGE010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258756"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2798)
                     /gene="treS"
                     /locus_tag="VD791_10940"
     CDS             complement(<1..2798)
                     /gene="treS"
                     /locus_tag="VD791_10940"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HXV08536.1"
                     /translation="MDRPERLEHAAATSTHTPPSSPLWFKDAVIYEVHVRAFCDSNGD
                     GIGDFPGLIQKLDYIQDLGVNAIWLLPFYPSPLRDDGYDIADYHDVHPDYGTLQDFRT
                     FVHEAHRRGLKVITELVINHTSDQHPWFQAARSAPPGSTKRDYYVWSDTPSRYAGTRI
                     IFTDTETSNWAWDETAKAYYWHRFFSHQPDLNFDNPHVLKAVVRAMRHWLDMGVDGLR
                     LDAIPYLCERAGTSNENLPETHALIKEVRRVIDQHYGDRMLLAEANQWPEDVRDYFAD
                     GDECHMAYHFPLMPRIYMAIAQEERHPIVEIMQQTPDIPDGCQWAIFLRNHDELTLEM
                     VTSRERDYMYRMYAADPRARLNLGIRRRLAPLMENDPGRIQLVNSLLLSMPGSPIIYY
                     GDEVGMGDNIFLGDRNGVRTPMQWSPDRNAGFSRADPQRLYLPPIMDPVYGFDAVNVE
                     AQSRDPSSLLNWMKRMLAVRRASQAFGRGTLRFLKPGNRKILAYLREHGEDAILCVAN
                     MSRTAQPVELDLARFRARVPVEMIGRTPFPPVGDLPYLLTLPGYGFYWFRLTVHAEVP
                     RWHEERSAREDLPVLVLFDGWNSFFRERVVPWRIGMAERMRAQLEQEALPHYLAVQRW
                     YAAKGALIARAALKDHAQLATARGNALLALLEVDAGGQRDAYFAPLLLAWEDGEEERV
                     RTLLPFTVARVRQQANVGVLADAFADQAFCLATLEAIGERLELPAGAGTFRFEPTAAF
                     EALSGEEPDELAVHPTQTQSSNTTVALGRRFFLKGYRLLRAGVNPELELGRFLTDVAR
                     FRNTVPLAGAVEYVGADGTRMTLALLQAYVENQGDAWSYTLDYLARFLEQRRTATESA
                     AADVHGVYLALMHTLGTRTAELHHALAQRTGDPLFDPEATTASDLAAWTNRVRDEIGA
                     SLDLLQQRRAELAEPARAL"
     gene            complement(2887..3888)
                     /locus_tag="VD791_10945"
     CDS             complement(2887..3888)
                     /locus_tag="VD791_10945"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HXV08537.1"
                     /translation="MLSACLAVAGVHAQADQVLLKNGDRITGRVLRKADDILSVDTDY
                     AGKLEIKWQEVSGLTTDAPVKVILPDDSGVDAKLGASAQGYARLQTGLTPAPIDLPLD
                     RIVYINPTPVQSGKGVSFGGRVNLGASTTSGNTDSKRLHAGAELRWRSRLNRYILGGE
                     INEGEDGGERTVSNWRAGGEYDYFFRPKNFFYTRATLEHDEFKDIDLRSTLGVGLGYQ
                     IFDTDRVELSVQGGLDYVNVERAVADDESYPAAGWGMRYDQQLFGGKVELFHTQDGFW
                     SLEEVEDVVLRTRTGLRFPLADGLDASAQVNIDWDNDPAPGRESTDVATILGLGYGW"
     gene            complement(3983..4450)
                     /locus_tag="VD791_10950"
     CDS             complement(3983..4450)
                     /locus_tag="VD791_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HXV08538.1"
                     /translation="MKLSTLLFLLTLAAITAFSVANWNVIVAPTSISLIFATVEAPLG
                     LILLGLVALVTALFLGFLVYLQTSVLIESRRHARQLEAQRQLADQAEASRFTDLRAFL
                     DAELKAMSAGLDRVEQELKGTVEQASNTLAAYIGELEDRLQRDAGVPTSDSAT"
     gene            complement(4493..>5047)
                     /locus_tag="VD791_10955"
     CDS             complement(4493..>5047)
                     /locus_tag="VD791_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HXV08539.1"
                     /translation="NGFMVRLVLAATLGASYGIYGPPFELMEHEPREPGSEEYLDSEK
                     YQLRHWDLARPDSLSGFIGRVNRARHDNPALHSDATLRFHDVDNDELVCYSKHTEDLS
                     NVVLAVVNLDPHHAQSGWLSLDLDALGLDPAQPFQAHDLLTGARFLWHGARNYVALDP
                     ARGPAHILLLRRRVHREQDFDYFM"
BASE COUNT          792 a         1682 c         1729 g          844 t
ORIGIN      
        1 agcgcacgtg ccggttcggc gagctcggcg cgacgctgct gcaacaggtc caggctcgcc
       61 ccgatctcgt cgcgaacgcg attcgtccag gcggcgagat ccgacgccgt ggtcgcttcg
      121 ggatcgaaca gcggatcgcc ggtgcgctgc gcgagcgcgt gatgcagctc ggcggtgcgc
      181 gtgcccagcg tgtgcatcag cgcaaggtac acgccgtgga cgtcggcggc ggccgactcg
      241 gtggcggtgc gccgttgctc caggaaacgc gccagatagt cgagcgtgta cgaccaggcg
      301 tccccctggt tctcgacgta ggcctgcagc aacgccagag tcatgcgcgt tccgtccgca
      361 cccacgtact ccactgcgcc ggccagcggc acggtgttgc ggaagcgggc tacgtcggtg
      421 aggaagcggc cgagctcgag ctcgggattg acgcccgcgc gcagcagccg atagcccttc
      481 aggaagaagc gccggcccaa cgccaccgtg gtgttgctgc tctgggtctg cgtcggatgc
      541 accgcaagct cgtcgggctc ctcgccgctc agcgcctcga atgccgcggt gggctcgaag
      601 cggaaggtgc cggcgccggc cggcaactcc agccgctcgc cgatcgcctc cagcgtcgcc
      661 aggcaaaagg cctggtcggc gaaggcgtct gccagcacgc cgacattcgc ctgctggcgc
      721 acgcgcgcca ccgtgaacgg cagcagggtg cgcacgcgct cctcctcgcc gtcttcccag
      781 gcgagcagca gcggggcgaa gtaggcgtcg cgctgcccgc cggcatccac ctccagcagc
      841 gccagcaggg cattgccgcg agcggtggcg agctgcgcat ggtccttcag cgcggcgcgt
      901 gcgatcagcg cgcccttggc ggcataccag cgctgcaccg ccagatagtg cggcagcgcc
      961 tcctgctcga gctgagcgcg catcctctcc gccatgccga tgcgccaggg caccacgcgc
     1021 tcgcggaaga agctgttcca gccgtcgaac agcaccagca ccggcaggtc ctcacgcgcg
     1081 ctgcgctcct cgtgccagcg cggcacctcc gcgtgcaccg tcaggcggaa ccagtagaag
     1141 ccgtagcccg gcagcgtgag caggtaaggc agatcgccca ccgggggaaa cggcgtgcgc
     1201 cctatcatct ccaccggcac gcgggcgcga aagcgggcga gatccagctc gaccggctgc
     1261 gctgtgcgcg acatgttggc cacgcacagg atggcgtcct cgccgtgctc gcgcagatag
     1321 gcgaggatct tgcggttgcc gggcttgagg aatcgcagcg tgccgcggcc gaaggcctgg
     1381 cttgcgcgcc gcaccgccag catgcgcttc atccagttga gcagcgagga gggatcgcgc
     1441 gactgcgcct cgacgttgac cgcatcgaag ccgtacaccg ggtccatgat cggcggcaga
     1501 tacaggcgct gcggatccgc gcgcgagaaa ccggcattgc gatccgggct ccactgcatg
     1561 ggcgtgcgca caccgttacg atcgcccagg aagatgttgt cgcccatgcc gacctcgtcg
     1621 ccgtagtaga tgatcggcga gccgggcatg gacagcagca ggctgttgac cagctggatg
     1681 cggcctggat cgttctccat cagcggggcc agccgccggc ggatgccgag gttgaggcgc
     1741 gcgcgcgggt cggccgcgta catgcggtac atgtagtcgc gctcgcggct ggtgaccatc
     1801 tcgagcgtca gctcgtcgtg gttgcgcagg aagatcgccc actggcagcc gtcggggatg
     1861 tccggggtct gctgcatgat ctccacgatc gggtgccgct cctcctgcgc gatcgccatg
     1921 tagatgcgcg gcatcagcgg gaagtgatag gccatgtggc actcgtcgcc gtcggcgaag
     1981 tagtcgcgca cgtcctccgg ccactggttg gcctcggcca gcagcatgcg gtcgccgtaa
     2041 tgctggtcga tcacacgccg cacctctttg atcagcgcgt gcgtctccgg caggttctcg
     2101 ttgctggtgc ccgcgcgttc gcacagatag gggatggcat cgaggcgcag gccgtccact
     2161 cccatgtcca gccagtggcg catggcgcgc accaccgcct tcagcacgtg cgggttgtcg
     2221 aagttcaggt ccggctgatg gctgaagaag cgatgccagt aataggcctt ggcggtctcg
     2281 tcccaggccc agttggaggt ctcggtgtcg gtaaagatga tgcgcgtgcc ggcgtagcgg
     2341 ctcggcgtgt cgctccacac gtagtagtcg cgcttggtcg agccgggcgg cgcgctgcgc
     2401 gctgcctgga accacgggtg ctggtccgaa gtgtgattga tcaccagctc ggtgatcact
     2461 ttgaggccgc ggcgatgcgc ctcgtgcacg aacgtgcgga agtcctgcag ggtgccgtag
     2521 tccgggtgga cgtcgtgata atcggcgatg tcgtagccgt cgtcgcgcag cggcgagggg
     2581 tagaagggca gcagccagat ggcgttgacg cccaagtcct ggatgtaatc gagcttctgg
     2641 atcagcccgg gaaagtcacc gatgccgtcg ccgttgctgt cgcagaaggc gcgcacgtgc
     2701 acttcgtaga tcacggcatc tttgaaccac agcgggctgc ttggcggcgt gtgcgtgctg
     2761 gtcgccgcgg cgtgctcgag gcgttcggga cgatccatgg ccggctggtc ttgctcctgt
     2821 agggaaaatc gatccgctag tttactcacc ctcggccggc cgcaggcgct gccggggggc
     2881 gcgcgctcac cacccgtaac cgagtccgag gatggtcgcc acgtcggtgc tctcgcgccc
     2941 gggcgccggg tcgttgtccc agtcgatgtt gacctgcgcc gaggcatcga gtccgtcggc
     3001 aagcgggaac cgcagaccgg tgcgcgtgcg caggaccacg tcctcgacct cctccaggct
     3061 ccagaagccg tcctgggtgt ggaacagctc gaccttgccg ccgaacagct gctggtcgta
     3121 gcgcatcccc cagcctgccg ccgggtagct ctcgtcgtcg gccaccgcgc gctccacgtt
     3181 cacgtagtcc aaccccccct gcacggacag ctccacgcgg tcggtgtcga aaatctgata
     3241 tccgagtccg accccgagag tggagcgcag gtcgatgtcc ttgaactcgt cgtgctcgag
     3301 cgtggcgcgc gtgtagaaga agttcttggg ccggaagaag tagtcgtact ctccgccggc
     3361 ccgccagttg gatacggtac gctcgccgcc atcctcgccc tcgttgatct cgccgcccag
     3421 gatgtagcga ttcagccgcg aacgccacct gagctcggcg ccggcatgca ggcgcttgct
     3481 gtcggtgttg ccgctggtgg tcgaggcgcc caggttgacg cgcccgccga agctcacgcc
     3541 cttgcccgac tgcaccgggg tcgggttgat atagacgatc cgatccaggg gcaagtcgat
     3601 cggcgccggc gtcagtccgg tttgcaggcg cgcgtatccc tgcgccgacg cgcctagctt
     3661 agcgtccact cccgagtcgt ccggcaggat cactttcacc ggtgcgtcgg tggtgagtcc
     3721 gctcacctcc tgccatttta tctcgagctt tcccgcgtag tcggtgtcca cgctgagaat
     3781 gtcgtcggcc ttgcgcagca cccgaccggt gatgcgatcg ccgttcttca gcagcacctg
     3841 atcggcctgc gcgtgcacgc cggcgacggc gaggcaggcc gacagcacac gaccgagaca
     3901 acgcgcacgg cattttgtca acaccatggg gagacagccg cggcgatatc gagttccccg
     3961 cggcgccgcc cgtcgaatcg actcaagtcg cgctgtccga ggtcgggacg ccggcgtcgc
     4021 gctggagccg gtcctcgagc tcgccgatgt aggcggcgag cgtgttgctc gcctgctcca
     4081 ccgtgccttt gagctcctgc tcgacgcggt ctaggccggc gctcatcgcc ttgagctcgg
     4141 cgtccaggaa tgcgcgcaga tcggtgaagc gcgatgcctc cgcttgatcc gcgagctggc
     4201 gttgcgcctc gagctggcgc gcgtggcggc gcgactcgat cagcaccgag gtctgcaaat
     4261 acaccaggaa gccgaggaac aaggcggtga ccagcgcgac cagcccgaga aggatcagcc
     4321 cgagcggagc ctcgacggtg gcgaagatca gggagatcga ggtaggcgcc acgatcacgt
     4381 tccagttcgc caccgaaaag gcggtgatcg cagccagggt cagcaggaac agcagggtgc
     4441 tcagcttcat cttggtctcc tcctctcgcg caggctgcag ccacttccga tcctacatga
     4501 agtagtcgaa gtcttgctct ctgtgcaccc gacgccgcag caacaggatg tgcgccggcc
     4561 cgcgcgccgg atcgagcgcc acgtaattgc gggcgccgtg ccacaggaag cgcgcgccgg
     4621 tcagcagatc gtgcgcctgg aacggttgcg ccgggtccag gccgagggcg tccaggtcga
     4681 ggcttagcca gccgctctgc gcgtgatgcg gatccaggtt gaccacggcg agcacgacgt
     4741 tggacaggtc ctcggtatgc ttgctgtagc agaccagctc gtcgttgtcg acgtcgtgga
     4801 agcgcagcgt ggcgtcggag tgcagcgccg gattgtcgtg gcgggcgcgg ttcactcgcc
     4861 cgatgaaccc gctcaagctg tccggccggg cgagatccca gtggcgcagc tgatacttct
     4921 ccgaatcgag gtactcctcc gagcccggtt cgcgcggctc gtgctccatc agctcgaagg
     4981 gcgggccata gatgccgtag ctcgcgccca gcgtggcggc gagcaccagc cgcaccatga
     5041 agccgtt
//
LOCUS       DASPGE010000340         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1493069, whole genome shotgun sequence.
ACCESSION   DASPGE010000340 DASPGE010000000
VERSION     DASPGE010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493069"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /gene="galU"
                     /locus_tag="VD791_10960"
     CDS             complement(<1..814)
                     /gene="galU"
                     /locus_tag="VD791_10960"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HXV08540.1"
                     /translation="MTSKTKRVRKAVLPAAGLGTRFLPATKAQPKEMLPVVDKPLIQY
                     SVEECLASGIENIIIVTGRGKNAIEDHFDNSPELEQFLANNNKPELARQVRHVSNMIH
                     VSYIRQKEPLGLGHAVLTAKELVGDEPFAVLLGDVIIDGPRPATRQLVDVYEATGLGA
                     IAVEEVPREQVHLYGVVDATPSDDKKWNARLLRVNNLVEKPKADAAPSNLAVTGRYVL
                     PPEIFDILEKTPPGKGGEIQLTDGLQTLARQSALYGLEFEGKRFDAGERLGFI"
     gene            complement(874..1782)
                     /locus_tag="VD791_10965"
     CDS             complement(874..1782)
                     /locus_tag="VD791_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08541.1"
                     /translation="MDRTLGREFEAIARDSVSGAADLTLRAAAALDAWLRRREPSAMD
                     VLLAARRACEAQPSMAPMLRLANDLAAAAESPRRLGDAQRALRGVTRTLRRAPQSIAR
                     HMSVLLRAPVKWQVVTYSASSTVLAALRRARRRIGSVFCSEGRPRFEGRALAVQLARA
                     RIPVVMATDAALLTEPPLGPGTAVVLGADAVLTGYFINKVGTSLLVDRAVAAGAPVFV
                     LADTMKFWPESPFRSTVWRWTYGHPHEVWTGAPRLVRSANPTFDATRLRPGIHVVTEH
                     GPWTTPRVRRHLERLRISRNLLAALD"
     gene            1830..>2199
                     /locus_tag="VD791_10970"
     CDS             1830..>2199
                     /locus_tag="VD791_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="universal genome maintenance protein;
                     Kae1/Qri7/OSGEP/YgjD family protein; in Archaea, some Kae1
                     are found as fusion proteins similar to two distinct
                     proteins in yeast that are involved in the KEOPS complex;
                     kinase associated endopeptidase 1 (Kae1) and a
                     serine/threonine protein kinase (Bud32); in Pyrococcus
                     Kae1 has atypical AP endonuclease activity and inhibits
                     the kinase activity of Bud32; the protein is essential in
                     Escherichia coli and Bacillus subtilis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HXV08542.1"
                     /translation="MTITRILGIESSCDETAAAVVADGREILSSIVASQVDIHRKYGG
                     VVPELASREHLRRIVPVVREALERAGATPADLDAIAVGHRPGLIGSLLVGCAAAKAMA
                     WSLGVPLIGVDHIVAHLHAAR"
BASE COUNT          310 a          758 c          787 g          344 t
ORIGIN      
        1 cgatgaagcc gaggcgctcg ccggcgtcga agcgcttgcc ctcgaactcg agcccgtaga
       61 gcgctgactg acgcgccagc gtctgcaacc cgtcggtcag ctggatctcg ccgcccttgc
      121 cgggtggggt cttctcgagg atgtcgaaga tctccggcgg cagcacgtag cgcccggtga
      181 ccgccaggtt cgacggcgcg gcgtcggcct tgggcttttc caccagattg ttgacgcgca
      241 gcaggcgggc gttccacttc ttgtcgtccg acggggtggc gtccaccacg ccgtagaggt
      301 gcacctgctc gcggggcact tcttccacgg cgatggcccc caggccggtg gcctcgtaga
      361 cgtccaccag ttggcgcgtg gccgggcgcg gcccgtcaat gatgacgtcg cccagcagca
      421 cggcaaaagg ctcgtcgccg accagttctt tcgccgtcag cacggcgtgg cccagcccca
      481 gcggctcctt ctgccggatg taactcacat ggatcatgtt gctgacgtgc cgcacctgcc
      541 gggccagctc cggcttgttg ttgttggcca ggaactgttc gagctccggc gagttgtcga
      601 agtggtcctc gatggcattc ttgccgcgcc cggtgacgat gatgatgttc tcgatgcccg
      661 aggccaggca ttcctcgacc gaatactgga tcagcggctt gtccaccacc ggaagcattt
      721 ccttgggctg ggccttggtg gccggcagga agcgcgtgcc cagtcccgcg gcgggcagta
      781 cggccttgcg tacgcgtttg gtcttcgatg tcatgtgttg ccgttctacg gccggcggcg
      841 aagtagcaag atacgcaacg cgcgcagcag gtgtcaatcc agcgcggcca gcaggttgcg
      901 cgaaatgcgc aaacgctcca ggtggcggcg cacgcgcggc gtggtccacg ggccgtgttc
      961 ggtgaccacg tggatgcccg gccgcaggcg cgtagcgtcg aaggtgggat tggcgctgcg
     1021 caccaggcgc ggtgcgccgg tccacacctc gtggggatgg ccgtaggtcc agcgccacac
     1081 ggtcgagcgg aagggcgact ccggccagaa cttcatcgta tccgccagta cgaacaccgg
     1141 cgcgcccgcg gccacggcgc ggtccaccag caggctggtg ccgaccttgt tgatgaagta
     1201 tccggtcaaa acggcgtcgg cgcccagcac cacggcggtg cccgggccca gcggcggttc
     1261 ggtcagcagc gccgcgtcgg tagccatgac cacgggaatc ctggcccgcg cgagctgcac
     1321 cgccagcgcc cgaccttcga agcgcggccg cccttcgctg cagaagacgc tgccgatgcg
     1381 gcgccgcgcc cggcgcagcg cggccagcac ggtcgagctc gccgagtagg tcaccacctg
     1441 ccatttcacc ggcgcgcgca gcagcacgct catgtggcgg gcgatggact gcggggcgcg
     1501 gcgcagagtc cgggtgacgc ctcgcagcgc gcgttgcgcg tcgccgagcc gccgcggcga
     1561 ctccgcagcc gcggccaggt cgttggccag gcgcagcatg ggggccatcg agggctgcgc
     1621 ctcgcaggcc cgccgcgcgg ccagcagcac gtccatcgcg ctgggctcgc gccggcgcag
     1681 ccaggcgtcc agcgccgcag cggcgcgcag cgtcaggtcc gcggcgccgg agacggagtc
     1741 gcgggcgatg gcctcgaatt cgcgtccgag ggtgcggtcc ataggagtgt cactctgcgg
     1801 gcgtatgcta acatgacccg cgaaggctta tgacaattac ccgcattctg ggcatcgagt
     1861 cgtcctgcga cgaaaccgcc gccgcggtgg tggccgacgg gcgcgagatc ctgtcgtcca
     1921 tcgtggcctc gcaggtggac atccaccgca agtacggcgg cgtggtgccc gagctggctt
     1981 cgcgcgagca cctgcggcgc atcgtgccgg tggtgcgcga ggcgctcgag cgggccggcg
     2041 cgacgccggc cgatctcgac gcgatcgccg tcgggcaccg gccgggactc atcgggtcgc
     2101 tgctggtcgg ctgcgccgcc gcgaaggcga tggcgtggtc cctcggcgtg ccgctcatcg
     2161 gcgtcgacca catcgtcgcg cacctgcacg cggcgcggc
//
LOCUS       DASPGE010000341         2860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1612110, whole genome shotgun sequence.
ACCESSION   DASPGE010000341 DASPGE010000000
VERSION     DASPGE010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612110"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VD791_10975"
     CDS             complement(<1..935)
                     /locus_tag="VD791_10975"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HXV08543.1"
                     /translation="MSAVIAPKAPTDIEALLGEEARSLLGHVCKGIPKEALNLPGPDF
                     VDRVVAPSDRSPSVMRSLQQIFGHGRLAGTGYVSILPVDQGIEHSAGASFARNPPMFD
                     PENIVKLAIEGGCNAVASTLGVLGAVARRYAHKIPFLLKFNHNEFLTYPNKYDQIFFA
                     GVKQARDMGAVAVGATIYFGSAESSRQIVEVSQAFAVAHEMGMATVLWCYLRNSHFKT
                     DKDYHLSADLTGQANHLGVTIEADIIKQKLPENNGGYNALSTKENPYGKTDKRVYTEL
                     TSDHPIDLTRYQVANCFMGRAGLINSGGASGENDF"
     gene            1062..1583
                     /locus_tag="VD791_10980"
     CDS             1062..1583
                     /locus_tag="VD791_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HXV08544.1"
                     /translation="MKYVLGERRVECRGEHYIAPNATVIGSVVLESEVSVWFNAVLRG
                     DNDLIRVGARSNIQDGAVLHTDPGIELSVGRECTIGHMVMLHGCSIGDGSLIGIKSVI
                     LNRARVGRNCLVGANSLITEGKEIPDRSLVVGSPAKVVRALTDEEVAFLLWNAEHYVE
                     KLRRYSRALQPQD"
     gene            complement(1600..2550)
                     /locus_tag="VD791_10985"
     CDS             complement(1600..2550)
                     /locus_tag="VD791_10985"
                     /inference="COORDINATES: protein motif:HMM:NF017519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HXV08545.1"
                     /translation="MKKVIRRIGSSIGRAIGRYLSEPVANYRPLTTCPSDWLAPILKP
                     GDVLLVEGNTRVSAAIKYLTQSTWSHACLYIGAALAPDATAEDAPVLIEVDMVGGVWA
                     VPLSKYAAFHTRVCRPALITVDDTQRVVDYALSRLGHTYDLKNMIDLARYLLPLPPLP
                     SRWRRRMLALGSGDPSKAICSTLIAQAFQSVGYPILPAMRRRHSDPGAEDHNREILTI
                     RHYSLYTPRDFDISPYFEVVKPTVQHGFSYRGIERERATTEPVEPSREGVVTAAARAP
                     AAAAPAAAAAAAAVNAGTNVPAPRESELELISRLDAESTA"
BASE COUNT          435 a          958 c          994 g          473 t
ORIGIN      
        1 tggaagtcgt tctcgcccga ggccccgccc gagttgatca accccgcgcg gcccatgaag
       61 cagttggcca cctgatagcg ggtgagatcg atgggatggt cgctggtgag ctccgtgtac
      121 acgcgcttgt cggtcttgcc gtacgggttc tccttggtgc tgagcgcgtt gtagccgccg
      181 ttgttctcgg gcagcttctg cttgatgatg tcggcctcga tggtcacgcc caggtggttg
      241 gcctgtccgg tcaagtcggc ggacagatgg tagtccttgt cggtcttgaa atggctgttg
      301 cgcaggtagc accacagcac ggtggccatg cccatctcgt gcgcgacggc gaaggcctgg
      361 ctcacctcga cgatctggcg cgaggactcg gccgagccga agtagatggt cgcgcccacc
      421 gcgaccgcgc ccatgtcgcg cgcctgcttc acgccggcga agaagatctg gtcgtacttg
      481 ttcgggtagg tgaggaactc gttgtggttg aacttgagca ggaacgggat cttgtgcgcg
      541 tacctgcgcg ccaccgcgcc cagcacgccc agggtggagg ccaccgcgtt gcagccgccc
      601 tcgatcgcca gcttgacgat gttctcggga tcgaacatgg gcgggttgcg ggcgaaggac
      661 gcgccggcgc tgtgctcgat gccctggtcc accggcagga tcgatacgta gccggtcccg
      721 gccagccggc cgtggccgaa gatctgctgc aggctgcgca tcaccgacgg ggagcggtcg
      781 ctcggcgcca ccacgcggtc gacgaagtcc ggtcccggga ggttcagcgc ctccttgggg
      841 atgcccttgc acacgtggcc gagcaggctg cgggcctcct cgcccagcag ggcctcgatg
      901 tcggtgggtg ccttgggtgc gatgacggcg ctcatggcct gcctccttcg atccggtcga
      961 aagcgtgatt atcgcgcgcc cgggagcgct gcgcgagccc gctataatgg cccgcatccg
     1021 atcgccctcg aaaccccgcc ctcgaaatca gggagccgcc cttgaagtac gtcctcggcg
     1081 agcgccgcgt ggaatgccgc ggcgagcact acatcgcgcc caacgccacc gtcatcggct
     1141 cggtggtgct ggaaagcgag gtgagcgtgt ggttcaacgc cgtgctgcgc ggcgacaacg
     1201 atctgatccg cgtgggcgcg cgcagcaaca tccaggatgg cgcggtgctg cacaccgacc
     1261 cgggtatcga gttgagcgtg ggccgcgagt gcaccatcgg ccacatggtg atgctgcacg
     1321 gctgcagcat cggcgacggc agcctgatcg gcatcaagag cgtgatcctc aaccgcgccc
     1381 gcgtcggccg caactgcctg gtcggggcca actcgctgat caccgagggc aaggagatcc
     1441 cggaccgcag cctggtggtc ggctcgccgg ccaaggtggt gcgggcgttg accgacgagg
     1501 aggtcgcgtt cctgctgtgg aacgccgagc actacgtcga gaagctcagg cgctacagcc
     1561 gggcgctgca gccgcaggac tgaagaggca agcgcgcggc taggcggtcg attcggcgtc
     1621 caggcgcgag atcaactcga gctcgctctc gcgcggagct ggcacgttcg tgccggcatt
     1681 gacggcggcc gctgccgctg ccgcggccgg cgccgccgca gccggtgcgc gggcagcggc
     1741 ggtcacaacg ccctcccggc tcggctcgac cggctccgtg gtggcccgct cgcgctcgat
     1801 gccgcggtag ctgaagccgt gctgcacggt gggcttcacc acttcgaaat acggcgagat
     1861 gtcgaagtcg cgcggcgtgt acaggctgta gtggcggatg gtgaggatct cgcggttgtg
     1921 gtcctcggca ccgggatcgc tgtggcggcg gcgcatcgcc ggcaggatcg gataacccac
     1981 cgattggaag gcctgcgcga tcagcgtgga gcagatcgcc ttgctcgggt cgccgctgcc
     2041 cagcgccagc atgcgccggc gccagcggct cggcagcggc ggcagcggca agaggtagcg
     2101 cgccaggtcg atcatgttct tcaggtcgta ggtatgaccc aggcgcgaca gggcatagtc
     2161 cacgacccgt tgcgtatcgt cgacggtgat cagcgccggc cggcagacgc gtgtgtgaaa
     2221 tgctgcgtac ttgctgagcg gaaccgccca caccccgccg accatgtcca cctcgatcag
     2281 caccggcgca tcctcggcgg tggcgtccgg cgccagcgcg gcgccgatgt acaggcaggc
     2341 gtgcgaccag gtcgactggg tgaggtactt gatggcggcg cttacccggg tgttgccctc
     2401 gaccagcagc acgtcgccgg gtttgaggat cggagccagc cagtccgagg ggcaggtggt
     2461 cagcggccgg taattggcga cgggctcgct cagataacgc ccgatcgccc gtccgatcga
     2521 gctcccaatt cggcggatca ccttcttcac ctcgtctcgg cagttgccgc agccctcata
     2581 gcccctcaag catcaactgg accgtggccg ccgcttcctc gcatcgtgcc cgcggcgcgc
     2641 cgcccgcgag cgcgccggac atgttgctac tgtgccgcgc ccggcgcgcc ggcgcaacta
     2701 gatctgctcg ccgcgcatgc cgctctgtcg ccggccgccg cccggcctgt acacccgtga
     2761 tagaaaattg acgaatccgg cggcgccccg cccggaaccg cctcctgacg tcgccccccg
     2821 aagacgccgc gtgcgcccct caagcaactg gcatggaacc
//
LOCUS       DASPGE010000342         2727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_558105, whole genome shotgun sequence.
ACCESSION   DASPGE010000342 DASPGE010000000
VERSION     DASPGE010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558105"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2588)
                     /locus_tag="VD791_10990"
     CDS             complement(<1..2588)
                     /locus_tag="VD791_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXV08546.1"
                     /translation="MQAVTLDAKYVATSGRIYLSGVQALVRLMLVQRLRDRAAGLHTA
                     GFVSGYRGSPLGGLDEALWKAQNHLEDGGVKFVPGVNEELAATAVWGTQQVNLIGPSD
                     YDGVFAMWYGKGPGVDRCGDVFKHMNFAGTARHGGVLLVAGDDHGAYSSTLPHQSDHL
                     FAASMIPVLYPCNVQEYLELGLHGFAMSRFSGCAVAFKALADTVESSASIDADPFALQ
                     ISLPEDFAVPESGLNSQLLSGLLAAQARQQEALMQDYKIYAAIAYARANRFNRVTIDS
                     PTARLGIVASGKSYMDVLEALEELGISAQNAGEVGIRLFKVGMPWPLEPDGVREFARG
                     LEEILVVEEKRQVVEYQLKEQLYNWQPDVRPRVIGKFDERGEWVHPRGRWLLPPKGDF
                     SVAQIARVIAERVARFHPSDLIKARLAFLEAKEAVLGKAVSTPVRPAYYCSGCPHNRS
                     TKVPDGSIALAGIGCHVMATFIYPEHTQSTTQMGGEGVPWIGQAPFSKREHVFANLGD
                     GTYFHSGQLAIRAAVAAGVNITYKILYNDAVAMTGGQPIDGTLTVPMIAQQMAAEGVK
                     RIALVAEDPARYADRGQLPQLVTLHDRTQLDFVQRELREFKGVSVLIYDQTCAAEKRR
                     RRKRGEYPQAHSRVFINELVCEGCGDCGVKSNCVSILPLETEFGRKRTIDQSSCNQDY
                     SCIEGFCPSFVTVEGAQVRKSTAGHASLGSQFPALADPQVPALDGPYNILITGIGGTG
                     VITIGALLGMAAHLEGKGVCVLDMTGMSQKNGAVTSHVRIAPDPEAIHAQRIATGEAN
                     LILGCDMLTAGAQDAIGKTRPGTTVAVINTYEQPPGSFAHSPDWQFPAEQVRALIDES
                     VAGRSH"
BASE COUNT          424 a          911 c          939 g          453 t
ORIGIN      
        1 tagtgcgagc gaccggccac ggattcgtcg atcagtgcgc gcacctgctc ggccgggaac
       61 tgccagtcgg ggctgtgggc gaaagagccg ggcggctgct cataggtgtt gatcaccgcc
      121 acggtggtgc cgggacgagt cttgccgatg gcgtcctggg cgcccgcggt cagcatgtcg
      181 cagccgagga tcagattcgc ctcgccggtg gcgatgcgct gcgcgtgaat cgcttccggg
      241 tccggagcga tgcgcacgtg cgaggtgacc gcgccgttct tctgcgacat gccggtcatg
      301 tcgagcacgc agacgccctt gccctccagg tgcgctgcca tgccgagcag cgcgccgatg
      361 gtgatgacac cggtcccacc gatgccggtg atgaggatgt tgtacggccc gtccaacgcc
      421 gggacctgcg gatcggccag cgccgggaac tgcgagccga ggctggcgtg cccggccgtc
      481 gatttgcgca cctgcgcgcc ctcgaccgtc acgaagctcg ggcagaagcc ttcgatgcag
      541 gaataatcct ggttgcagga ggactggtcg atggtgcgct tgcggccgaa ttcggtctcg
      601 agcggcagga tcgacacgca gttggacttc accccgcagt cgccgcagcc ttcgcacacc
      661 agctcgttga tgaacacgcg cgagtgcgcc tgcgggtatt cgcccctctt acgccggcgc
      721 cgcttctcgg cggcacaggt ctggtcgtag atcagcaccg acacgccctt gaactcgcgc
      781 agctcgcgct ggacgaaatc gagctgcgtg cgatcgtgca gcgtcaccag ttgcgggagt
      841 tgcccgcgat ccgcatagcg cgccggatcc tccgcgacga gcgcgatccg cttcacgccc
      901 tcggcggcca tctgctgcgc gatcatcggc acggtcaagg tgccgtcgat gggctggccg
      961 ccggtcatcg ccacggcgtc gttgtacagg atcttgtacg tgatgttgac gcctgccgcg
     1021 acggcggcgc ggatcgcgag ctgtccggag tggaagtagg tgccgtcgcc gagattggcg
     1081 aacacatgct cgcgcttcga gaacggcgcc tgcccgatcc agggcacgcc ttcgccgccc
     1141 atctgcgtcg tgctctgcgt gtgctccggg tagatgaagg tcgccatgac gtggcagccg
     1201 atgccggcta gcgcgatgct gccgtcgggc actttggtcg accggttgtg cgggcagccc
     1261 gagcagtaat aggccgggcg caccggggtg ctcaccgcct tgccgagcac cgcctccttc
     1321 gcttccagga aggcgagccg ggccttgatc aggtcgctgg ggtggaagcg cgcgacgcgc
     1381 tcggcgatca cgcgcgcgat ctgcgcgacc gagaaatcgc ccttcggcgg cagcagccag
     1441 cggccgcgcg ggtgcaccca ttcgccgcgt tcgtcgaact tgccgatcac gcgcgggcgc
     1501 acgtcgggct gccagttgta gagctgctcc ttgagctggt actcgaccac ctggcgcttc
     1561 tcctccacca ccaggatctc ctccaggccg cgcgcgaact cgcgcacgcc gtcgggctcg
     1621 agcggccagg gcatgcccac cttgaacaga cggatgccga cctcgccggc gttctgcgcc
     1681 gatatgccca gctcctccag cgcttcgagg acgtccatgt acgacttgcc ggaagccacg
     1741 atgcccaggc gtgccgtggg cgagtcgatg gtcacgcggt tgaaccggtt ggcgcgcgcg
     1801 taggcgatgg ccgcatagat cttgtagtcc tgcatcagcg cctcctgctg gcgcgcctgc
     1861 gccgccagca gcccgctcag cagctgagag ttgagaccgc tctccggcac ggcgaagtcc
     1921 tccggcaggc tgatctggag ggcgaagggg tcggcgtcga tcgaagccga ggactcgacg
     1981 gtgtccgcaa gcgccttgaa cgccaccgcg cagccggaaa agcgcgacat ggcgaagccg
     2041 tgcagcccga gctcgaggta ttcctggacg ttgcagggat acaggaccgg gatcatcgaa
     2101 gccgcgaaca gatggtcgct ctggtgcggc agggtggagg agtacgcgcc gtggtcgtcg
     2161 ccggccacca gcagcacgcc gccgtgcctg gccgtgccgg caaagttcat gtgcttgaac
     2221 acgtcgccgc agcggtccac accggggcct ttgccgtacc acatggcgaa cacgccgtcg
     2281 tagtccgagg ggccgatcag attgacctgc tgcgtgcccc acacagcggt ggccgccagc
     2341 tcctcgttga cgcctggcac gaacttgacg ccgccgtcct cgaggtggtt ttgcgccttc
     2401 cagagcgctt cgtccagacc cccgagcggg gatccgcggt aaccggagac gaaaccggcc
     2461 gtatggagcc cggctgcgcg atcgcgcagg cgttgcacaa gcatcaggcg gaccagcgcc
     2521 tggacgccgc tcaggtagat gcggccgctg gtggcgacgt acttggcgtc caaggtgact
     2581 gcttgcatgg ccaaactcct cgtaggaatc cgacttcagg ccgtccgccc tggtgaggcg
     2641 acaggcccgt gtccgcgccg gcaagcgggt gggctgccag cgcgggcgcg actatagcgc
     2701 agcttcgcca gttggacatc gtggagc
//
LOCUS       DASPGE010000343         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1729897, whole genome shotgun sequence.
ACCESSION   DASPGE010000343 DASPGE010000000
VERSION     DASPGE010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729897"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /gene="pilV"
                     /locus_tag="VD791_10995"
     CDS             <1..484
                     /gene="pilV"
                     /locus_tag="VD791_10995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HXV08547.1"
                     /translation="NASSTLLRRHRESGFTMVEVLVALVVLAIGLLGIAALYLNSLQS
                     GRTAIYRTQAVNLAADLADRIRANRTAQNAYATAFADAEAVVAACDATGGCVDTDLAS
                     TDLARWKATLAQLLPNGAGQVVVTPPVGAGEPATYIVTVRWVEAGEAAPITFQIGFQT
                     "
     gene            487..1470
                     /locus_tag="VD791_11000"
     CDS             487..1470
                     /locus_tag="VD791_11000"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HXV08548.1"
                     /translation="MQRQRGMSLVELMVAMAIGLFLLAGAITVFGKTRDLYRTNDTAS
                     RLQETARYAMSTIEADLRMANYWGLMSRADLVENGPALDLANPPDVDPTYALPADLNA
                     YSATINSCGAMWAVKLPAYVEATDDAYGLGCAEFGTAMGGADTLTIRRASANLVPATA
                     GLIKVQSSRMQGSLFAGTALPAGYTAGLSETRALVAHGYYVDQASEMDPNMPSLRRKQ
                     LDFAGGAPVILDREVVPGVEDLQVEYGADLDNDQNADYFVQPGTAIPAGDQIVAVRIW
                     LLVRGEQGELGFVDSRTYDYASRTGAAAITPNDPFRRVLVSKTIALRNTRR"
     gene            1467..1916
                     /locus_tag="VD791_11005"
     CDS             1467..1916
                     /locus_tag="VD791_11005"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HXV08549.1"
                     /translation="MNGLHSAPASRQRGAALVVGLILLVVLTILAVSGVFTSTMELRM
                     ARNTQSQERAFQAAEVAVEDALGNPVLSTSTPFNQALTAVPNGGGDQYAYQLQFAGQT
                     PLGTGMTGFSIGSAFQAYHFQVNAAGTGPDNATAQHTQSFYVIGPGS"
     gene            1936..2325
                     /locus_tag="VD791_11010"
     CDS             1936..2325
                     /locus_tag="VD791_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08550.1"
                     /translation="MNHPDTRNLRRAARLLAGALVATAGALAMASAAAQARMQTVEQC
                     LESGTDLVTLPAIAGGTLSASECRGCPSLRLRFDARTRYYISGELVSYARLREAAARG
                     PLRLDVFYRPGDRTLTRLRLAAAATAQ"
     gene            2336..>2539
                     /locus_tag="VD791_11015"
     CDS             2336..>2539
                     /locus_tag="VD791_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08551.1"
                     /translation="MKPTTTRSFRIAASAALLALAAPALADDAEIFVGTGSVVSSQRP
                     NILFVMDTSGSMSTNVTTQVPYNA"
BASE COUNT          399 a          944 c          823 g          373 t
ORIGIN      
        1 gaacgccagt tccaccctgc tgcgccgtca ccgcgaatcg ggcttcacga tggtcgaagt
       61 gctggtcgcg ctcgtcgtgc tggcgatcgg cctgctcggc atcgcggcgc tctacctcaa
      121 cagcctgcag tccggccgca ctgcgatcta tcgcacccag gccgtcaacc tggccgccga
      181 cctcgccgac cgcatccgcg ccaaccgcac agcccagaac gcctacgcga cggcattcgc
      241 ggatgcggag gccgtggtgg ccgcctgcga cgccaccggc ggctgcgtcg acaccgatct
      301 cgcctccacc gacctggcgc gctggaaggc gacgctggcg caactgctgc cgaacggcgc
      361 gggccaggtg gtggtgacgc cgccggtggg cgccggggag ccggcgacct acatcgtcac
      421 ggtgcgctgg gtcgaggccg gcgaggctgc gcccatcacc ttccagatcg ggttccagac
      481 atgacgatgc aacggcagcg cggcatgagc ctcgtggaac tgatggtcgc catggccatc
      541 ggcctgttcc tgctcgcggg cgcgatcacc gtgttcggca agacgcgcga cctgtaccgc
      601 accaacgaca ccgcatcgcg cctgcaggag accgcgcgct acgccatgag cacgatcgag
      661 gccgacttgc gcatggccaa ctactggggc ctcatgagcc gcgcggatct ggtcgagaac
      721 ggcccggcgc tggatctggc caacccgccg gacgtggacc cgacctatgc cctgccggcg
      781 gacctgaacg cgtattccgc caccatcaat tcctgcggcg cgatgtgggc ggtcaagctg
      841 ccggcctacg tcgaggccac cgacgacgcc tatggcctgg gctgcgccga gttcggcacg
      901 gcgatgggcg gcgccgacac gctcaccatc cgccgggcgt ccgcaaatct ggtgccggcc
      961 acggcggggc tcatcaaggt gcagtcgagc cgcatgcagg gcagcctttt cgcgggcacc
     1021 gcgctgccgg ccggatatac cgccgggctt tccgagaccc gcgcgctggt cgcccatggc
     1081 tattacgtgg accaggcctc cgagatggac ccgaacatgc cgtcgctgcg ccgcaagcag
     1141 ctcgatttcg cgggcggcgc gccggtcatc ctcgaccgtg aagtcgtgcc cggcgtcgag
     1201 gacctgcaag tcgaatacgg ggccgacctc gacaacgacc agaacgccga ttatttcgtg
     1261 cagcccggca ccgcgattcc ggccggcgac cagatcgtcg ccgtgcgcat ctggctgctg
     1321 gtgcgcggcg aacagggtga actcggcttc gtcgacagcc gcacctacga ctatgcgagc
     1381 cgcacgggcg cggccgcgat cacgccgaac gacccgttcc ggcgcgtgct ggtcagcaag
     1441 acgattgccc tgaggaacac gcgtcgatga acggccttca ctccgccccg gcctcgcggc
     1501 agcgcggtgc agcgctggtc gtcggactca tcctgctggt cgtgctcacg atcctggccg
     1561 tgtccggcgt gttcacctcc accatggagc tgcgcatggc gcgcaacacg cagagccagg
     1621 agcgcgcgtt ccaggccgcg gaggtcgcgg tcgaggacgc gctcgggaac ccggtgctga
     1681 gcaccagcac gccattcaac caggctctca cggcggtgcc gaacggcggc ggcgaccagt
     1741 acgcctacca gctgcagttc gcgggccaga cgccgctcgg caccggcatg accggcttca
     1801 gcatcggttc tgcgttccag gcctaccact tccaggtcaa tgccgcgggc accgggccgg
     1861 acaacgccac cgcgcagcac acccagagct tctacgtcat cggcccgggc agctgattat
     1921 cctcggagca atcccatgaa ccaccccgat acccgcaatc tccgccgcgc tgcacgcctg
     1981 ctggccggtg cactggtcgc gaccgcgggc gctctcgcca tggcttcggc tgccgcccag
     2041 gcgcgcatgc agaccgtcga gcagtgcctg gaaagcggca ccgacctggt gacgctcccg
     2101 gccatcgccg gcggcacgct gagcgccagc gagtgccgcg gctgcccgtc gctgcggctg
     2161 cgattcgacg cgcgcacgcg ctattacatc tccggcgaac tggtgtccta cgcccgcctg
     2221 cgcgaagcgg cggcaagggg cccgctccgg ctcgacgtct tctaccgtcc cggcgaccgc
     2281 accctgacgc gcctgcgtct ggcagcggca gccaccgcac agtgagtgca ctgccatgaa
     2341 accgaccacc acgcgcagct tccgaattgc cgcttccgcc gcgctcctcg cgctcgcggc
     2401 gccggcgctc gccgacgacg ccgagatctt tgtaggcacc ggttccgtcg tgtcgagcca
     2461 gcgcccgaac atcctgttcg tcatggacac ctcgggcagc atgtcgacga acgtgaccac
     2521 gcaggtcccc tacaacgcg
//
LOCUS       DASPGE010000344         3511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_792708, whole genome shotgun sequence.
ACCESSION   DASPGE010000344 DASPGE010000000
VERSION     DASPGE010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792708"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..558
                     /locus_tag="VD791_11020"
     CDS             73..558
                     /locus_tag="VD791_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HXV08552.1"
                     /translation="MRAKPADAATRWVTRERPKIDRIACPWLIRRFIDPQAEFLYVPT
                     PEVRRAAAERDAVPYDVPDVEFSHRGELCSFDAFLATYRLTDPALAELALIVRGADTD
                     RHDLAPQAAGLAAVSLGLSRLYADDHAMLEQGMVLYDALYLWCKEGKSEVHTWNPAAY
                     R"
     gene            534..1877
                     /gene="chrA"
                     /locus_tag="VD791_11025"
     CDS             534..1877
                     /gene="chrA"
                     /locus_tag="VD791_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HXV08553.1"
                     /translation="MESGRVPLEAVRFTDALRFWLKLGFISFGGPTGQIAIMHQELVE
                     RRRWISENRFLHALNYCMVLPGPEAQQLATYIGWLMHRTWGGLAAGGLFVLPSVFILV
                     ALSWIYLAFGEVPLVAGLFYGIKPAVTAVVVAAAIRIGSRALRNAWLWGIAAAAFIAI
                     FALKVPFPAIVIGAGVIGYLGGRIAPDMFRAGAAHAGAQAGYGPALIDDATPAPAHAR
                     FSWVRFWSVIAAGLALWGLALGALAAFAGWHGTLTQMGWFFTKTALLTFGGAYAVLPY
                     VFQGAVEHFQWLSAAQMIDGLALGETTPGPLIMVVAFVGFVGGWNGALFGPEALFVSG
                     FVAALVVTLFTFLPSFVFIFAGAPFVETTHGNLKFTAPLAGITAAVVGVILNLALFFA
                     YHVLWPQGFEGRFEWVSALVGALAFVALLRYKVGVIPVIAACALAGLAARLAEIA"
     gene            1904..2194
                     /locus_tag="VD791_11030"
     CDS             1904..2194
                     /locus_tag="VD791_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HXV08554.1"
                     /translation="MAAYVIAEVEVTDPAAYEEYRKRVPATIEQYGGKYLVRGGATES
                     KEGGWTPARFVILEFPSMAQARRWYDSPEYAPALAIRKRASKSKLIFAEGLG"
     gene            complement(2359..2517)
                     /locus_tag="VD791_11035"
     CDS             complement(2359..2517)
                     /locus_tag="VD791_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08555.1"
                     /translation="MAQLSKWVARGAVAIALALTMTAVTGCASNGADADRQDQQWDRP
                     PGWVPFSA"
     gene            complement(2696..>3511)
                     /locus_tag="VD791_11040"
     CDS             complement(2696..>3511)
                     /locus_tag="VD791_11040"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HXV08556.1"
                     /translation="DTWRQIRNGPITVYGGWLVVLVALAIAAFYLVRGPIKTHDKPTG
                     NLIYRFSVLERWTHWTMAISFCLLAVTGLILLFGKHLLLPVIGYTLFAWLTLLGKNVH
                     NFVGPIFMASIVVFFLIFVKDNWPSKADWAWIASAGGLFTGKHVPSARFNAGEKSWFW
                     FGVVFLGIVSSVSGLILLFPNFEQLRLTMQQAHVVHSVSGILFMALALGHIYVGTIGI
                     EGSYQAMRTGYVDESWAKEHHELWYQEAKKQRANRPASGGAVPAGAAQARRDE"
BASE COUNT          527 a         1238 c         1163 g          583 t
ORIGIN      
        1 ctgaatgacg ccggtgtcgc ggcgcgctat ctcgagggcg gcatcgaggc ctggcgcagc
       61 cagggcggcg cgctgcgcgc caaaccggcc gatgccgcga cgcgctgggt gacgcgcgag
      121 cggccgaaga tcgatcgcat cgcctgtccc tggctcatcc ggcgcttcat cgatccgcaa
      181 gccgagttcc tctacgtgcc gacgcccgag gtgcgcaggg ctgcggccga gcgcgacgca
      241 gtgccctacg acgtcccgga tgtcgagttc tcacacagag gcgaactctg cagcttcgat
      301 gccttcctcg ccacctatcg cctgaccgac cccgcgctcg ccgagctcgc gctgatcgtg
      361 cgcggcgccg acaccgaccg gcacgatctc gctccgcagg cggccgggct ggccgccgtg
      421 tcgctcggcc tgtcgcgtct ctacgccgac gatcacgcga tgctcgagca gggcatggtg
      481 ctgtacgacg cgctttacct gtggtgcaag gaaggcaaga gcgaggtgca cacgtggaat
      541 ccggccgcgt accgctagag gcagtgcgct tcaccgacgc gctgcgcttc tggctcaagc
      601 tcggcttcat cagcttcggc gggccgaccg ggcagatcgc gatcatgcac caggagctgg
      661 tcgagcgccg gcgctggatc tcggagaacc gcttcctgca cgcgctcaac tactgcatgg
      721 tgctgcccgg ccccgaggcg cagcagctcg cgacctacat cggctggctg atgcaccgga
      781 cctggggcgg gctcgccgca ggcggcctgt tcgtgctgcc ttcggtgttc attctcgtcg
      841 ccctgtcctg gatctatctc gccttcggcg aggtgccgct ggtcgcggga ctgttctacg
      901 gcatcaagcc ggcggtgacc gcggtcgtcg tcgcggccgc gattcgcatc ggctcgcgcg
      961 cgctgagaaa cgcctggctg tggggcatcg cggctgcggc cttcatcgcg atcttcgcgc
     1021 tcaaggtccc gtttcccgcg atcgtgatcg gcgccggcgt gatcggctat ctgggcgggc
     1081 gcatcgcgcc ggacatgttc cgcgccggcg ccgcgcatgc cggcgcgcag gccggctacg
     1141 gccccgcgct gatcgacgac gcgacgcctg cgcccgcgca cgcgcgcttt tcctgggtgc
     1201 ggttctggag cgtcatcgca gccgggctcg cgctttgggg cctcgcgctc ggcgcgcttg
     1261 ccgcgttcgc cggatggcac ggaaccctca cacagatggg ctggttcttc accaagaccg
     1321 cgctgctcac cttcggcggc gcctatgccg tgctgcccta tgtgttccag ggcgcagtcg
     1381 agcacttcca gtggctgagc gcggcgcaga tgatcgacgg cctcgcgctg ggcgagacca
     1441 cgccgggacc gctcatcatg gtggtcgcct tcgtagggtt cgtgggcgga tggaacggcg
     1501 cgctgttcgg cccggaggcg ctcttcgtct ctggattcgt cgccgccctg gtggtgacgc
     1561 tgttcacctt cctgccctcg ttcgtgttca tcttcgcggg cgccccgttc gtcgagacga
     1621 cgcacggcaa cctgaagttc accgcgccgc tcgccggcat caccgcggcg gtcgtcggcg
     1681 tgatcctcaa cctggcgctg ttcttcgcct accacgtgct ctggccgcag ggcttcgagg
     1741 gccgcttcga gtgggtctcg gcgctcgtcg gcgcgctcgc cttcgtcgcg ctattgcgct
     1801 acaaagtcgg cgtcattccc gtgatcgccg cctgcgcgct tgccgggctg gcggcgcgcc
     1861 tcgcggaaat cgcctgagcg ccgaacgaac cgggagaacc ctgatggctg cctatgtgat
     1921 cgccgaagtc gaagtcaccg accccgccgc ctacgaggag taccgcaagc gggtaccggc
     1981 aacgatcgag cagtacggcg gaaagtacct ggtgcgcggc ggcgccaccg aaagcaagga
     2041 aggcggctgg acgccggcgc gcttcgtgat cctcgagttt ccctcgatgg cgcaggcgcg
     2101 gcgctggtac gactcgcccg agtacgcgcc ggcgctcgcg atccgcaagc gcgcgtcgaa
     2161 atcgaagctg atctttgccg aagggctagg ctgagaaggg cgtaaagccg ggctgctgaa
     2221 agtcgcggcg cggctcgtcg cgcggctcgc gccccggttg gctgccgtcg ctggcacagc
     2281 cgcctgccga cagcacggca agcgcgaagg caaccagtat cagccgtctc gacagtctgc
     2341 gcacgcctgg atcctacctc aagccgagaa cggaacccat ccgggcgggc gatcccattg
     2401 ctggtcctgt cggtccgcat cggcgccgtt gctcgcgcat cccgtcaccg ccgtcatcgt
     2461 caaggcgagc gcaatcgcca ccgcgccgcg cgccacccac ttcgaaagct gcgccataga
     2521 ccgccctcgc cagagtattg cgtcgcgcag ccgcgcttcg caaacgattg caggaaaaca
     2581 atcctatgcg atagacgcgc acaagccagc cgggttcccg gtcccgggcg cggacgcaag
     2641 gaccaccgcg gcggcgcgta ccacgccccg cgcaggtcct cccctccacc tccgcctatt
     2701 cgtcgcgccg cgcctgggct gcgcccgccg gcaccgcacc gccgctcgcg ggccggttgg
     2761 cgcgctgctt cttcgcttcc tggtaccaga gttcgtggtg ctccttggcc caggactcgt
     2821 cgacgtaacc ggtgcgcatc gcctggtagg acccctcgat gccgatcgtg ccgacgtaga
     2881 tgtgccccag cgccagcgcc atgaacagga tgccggatac cgagtgcacc acgtgcgcct
     2941 gctgcatcgt caggcgcagc tgctcgaagt tcgggaacag caggatcagc cccgacacgc
     3001 tcgagacgat gccgagaaag acgacgccga accagaacca ggatttctcg cccgcgttga
     3061 agcgcgccga gggcacgtgc ttgccggtga acagccctcc ggccgaggcg atccacgccc
     3121 agtctgcctt cgacggccag ttgtccttca cgaagatcag gaagaacacc acgatgctcg
     3181 ccatgaagat cgggccgacg aagttgtgca cgttcttgcc gagcagcgtg agccaggcga
     3241 acagcgtgta gccgatcacc ggcagcagca ggtgcttgcc gaacagcagg atgagcccgg
     3301 tgaccgccag caggcagaag ctgatcgcca tggtccagtg cgtccagcgc tcgagcacgc
     3361 tgaagcggta gatcaggttg ccggtcggct tgtcgtgcgt cttgatcggc ccgcgaacga
     3421 ggtagaacgc cgcgatcgcg agcgccacca gcaccaccag ccagccgccg tagaccgtga
     3481 tcggcccgtt gcggatctgg cgccaggtgt c
//
LOCUS       DASPGE010000345         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_441990, whole genome shotgun sequence.
ACCESSION   DASPGE010000345 DASPGE010000000
VERSION     DASPGE010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441990"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VD791_11045"
     CDS             complement(<1..413)
                     /locus_tag="VD791_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08557.1"
                     /translation="MCWMPVKRRMPVEYSSMPVDHRVHAPRRSLAALAGAAMLAFLPA
                     SARAVEVQNYGFEAAAQEVTQLYWLAETANVCGWASGDDILAFKLFSVRFLTAHLSER
                     NRLALVSLVTENGYEERVRKAALEGASHNCGSNRWR"
     gene            1039..1566
                     /locus_tag="VD791_11050"
     CDS             1039..1566
                     /locus_tag="VD791_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08558.1"
                     /translation="MKLHQIKIEFDAEQDRLRVRVSTLSGEEALLWLTRRCVLRLWPL
                     LVGGAEASPQVVTRAADPEVKKALLGFQHEKAISQAQFGKQYEDVPRTRPLGDAPLLV
                     TKLQRRAGPDGRIVLGLLPSQGQGIFLTLDEPLLHGFIKLLQQAVQRAEWGFNLQLPW
                     SGSSPEPGATERTLQ"
     gene            complement(1634..>2457)
                     /locus_tag="VD791_11055"
     CDS             complement(1634..>2457)
                     /locus_tag="VD791_11055"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXV08559.1"
                     /translation="QPLADRLAPHDVVLVIGAPVFTYHVHTEGVFVAPGTRLFHLTDD
                     PEQAARAPVGTSILSSIQLGMEQLLAQISQVRRAAPRIAPRPPAPPLADPIGGELAMH
                     TICSSLPADAVVVEEAPSHRNAFHAHFPIRVSGGLYAVASGGLGFALPAAVGVALADA
                     SRKVVAVLGDGSSLYTIQGLWTAAQHALPITFVILNNAGYGAVKSLAARMGIERMPGG
                     DIPGVDFVDIARGFGCRASRVERAAELAPALIEAHASDRPWLVDVRMDRSADKLY"
BASE COUNT          385 a          863 c          839 g          370 t
ORIGIN      
        1 cgccagcgat tgctgccgca gttgtggctg gcgccttcca gcgccgcctt gcgcacccgt
       61 tcctcgtaac cgttctcggt taccagcgag accagcgcca ggcggttgcg ctccgacaga
      121 tgcgcggtga ggaagcgcac cgagaacagc ttgaacgcga gaatgtcgtc cccgctcgcc
      181 cagccgcaga cgttggcggt ctcggccagc cagtagagct gggtgacctc ttgggcggcc
      241 gcctcgaacc cgtagttctg cacctcgacg gcgcgggcgg aagcaggcag gaaggcgagc
      301 atcgcggcgc ccgcgagggc ggccaacgat ctccgcggcg cgtgcacccg gtgatctacc
      361 ggcatcgaag aatactcgac cggcatacgg cgcttgactg gcatccaaca catggcgcga
      421 ctccttatcc atccacggcc cctccaccct gcgacagcag agctcggagc ggttgtgggt
      481 gacttcgtcc gcgatgcgtg ggcggctgcg agggcggccg gagccgtccc ggagtcgtgc
      541 atgcctggcg cgtgctcctg accccctatc aggcaaattg cgggcccgct tccggcccgg
      601 cgcggaccag cgtgcaaatt cgcggcacga acggcgcccg gggcgtggcc gccgtccaat
      661 ctgtctcttg ccgaatgctc tctgcgtgcc atctgcctgt gctcctcact gctcctcggc
      721 ccaccaggct ccacggtcgg atagttcccg atgctcgttc gctggtgaat atgtccaacg
      781 acagggatgc gtcggagccg gcgcccgagc cttacctccc ctgtggaaac gctacacaca
      841 cgttatgccc ggtgagaaat tcgcgacctg cccgtgaaac catcacgttt ccctcacgcc
      901 gcggcccccc ggagcgggcc cggaccctgc ttacggttca ggggttgagc acccgcgccc
      961 gcgggcagcc acgcgcacgg cgccgcgggc gtgccggtgc ttacgtgccg gccacccggc
     1021 gggtatcatt ggtcccggat gaagctgcac cagatcaaga tcgagttcga cgccgagcag
     1081 gatcgcctgc gcgtgcgggt gtccacgctg agcggcgagg aggccctgct gtggctgacg
     1141 cgtcgctgtg tgctgcggct atggccgctg ctggtgggcg gtgcggaggc gagccctcag
     1201 gtcgtcacgc gcgcggccga ccccgaggtg aagaaagcgc tgctgggctt ccagcacgag
     1261 aaagcgatca gccaggcgca gttcggcaag cagtacgagg acgtgccgcg cacgcggccc
     1321 ctgggcgacg ccccgctgct ggtgaccaag ctccagcgcc gcgccggccc cgacgggcgc
     1381 atcgtgctgg gactgctgcc gagccagggt cagggcatct ttctcacgct cgacgagccg
     1441 ctgttgcacg gcttcatcaa gctgctgcag caggcggtgc agcgcgccga gtggggtttc
     1501 aacctgcagc tcccgtggag cgggtcgtcg ccggaaccgg gagcgaccga gcgtacgctg
     1561 caatagagcg tgattcgtga ctcgtgattc gtgactcgtc acgaatcacc aatcacgatt
     1621 cacgaatcac gcatcagtag agcttgtccg cgctgcggtc catgcgcacg tccaccagcc
     1681 acgggcggtc cgatgcgtgg gcctcgatca gcgccggcgc cagctcggcg gcgcgctcga
     1741 cgcgcgaggc acggcagccg aagccccgtg cgatatcgac gaagtccacg cccggaatgt
     1801 cgccgcccgg catgcgctcg atacccatgc gcgccgccag agacttgacg gcgccgtatc
     1861 cggcgttgtt gaggatcacg aaggtgatcg gcaaggcgtg ttgcgcggcg gtccacagcc
     1921 cctggatcgt gtacaggctg gagccgtcac cgagcaccgc caccaccttg cgcgacgcat
     1981 cggccagcgc cacgcccacc gccgccggca gcgcgaagcc gagcccgccg ctcgccaccg
     2041 cgtacaggcc gccgctcacg cggatcggaa agtgcgcgtg gaacgcattg cggtgcgagg
     2101 gcgcctcttc caccaccacg gcgtcggcgg gcagagagct gcagatggtg tgcatcgcca
     2161 gctcgccccc gatcgggtcg gcgagcggcg gcgccggcgg gcgcggcgcg atgcgcggcg
     2221 ccgcgcgccg cacctgagag atctgcgcca gcaactgctc catgccgagc tggatgctgc
     2281 tcaggatgct ggtgccgacc ggcgcgcggg cggcctgctc cggatcgtcg gtcaggtgga
     2341 acagccgcgt gcccggcgcc acgaaaacgc cctcggtatg cacgtgatag gtgaacaccg
     2401 gcgcgccaat caccagcacc acgtcgtggg gtgcgagccg gtccgccagc ggctggc
//
LOCUS       DASPGE010000346         3527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1494785, whole genome shotgun sequence.
ACCESSION   DASPGE010000346 DASPGE010000000
VERSION     DASPGE010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494785"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..2720)
                     /locus_tag="VD791_11060"
     CDS             complement(135..2720)
                     /locus_tag="VD791_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HXV08560.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQKLVDLDIETAGKEQRKGNIYKG
                     VITRVEPSLEAAFVDYGSERHGFLPFKEVSRAYFLSDVDPARARIQDVMRDGQELIVQ
                     VDKDERGSKGAALTTFVSLAGRYLVLMPNNPRGGGVSRRVEGEERNELRDLIAALEVP
                     QGMSIIGRTAGLGRTLEELQWDLNYLLQLWRAIENASRQQSGAFLIYQESSLVIRAIR
                     DYFHQDIGELLVDTESIYEQARQFMAHVMPGNVNRVKLYKDDVPLFSRFQIEHQIETA
                     YARQVPLPSGGAIVIDHTEAMVAIDVNSARATRGSDIEETALRTNLEAADEIARQLRL
                     RDLGGLVVIDFIDMEQSRNQREVENRLRDGLRFDRARVQMGKISRFGLMELSRQRLQT
                     SLGETTHMACPRCNGIGHIRSVESSALHILRILQEEAMKENTAAVHAQVPVDVATFLL
                     NEKRAEFHALEQRLKVNVVLIPNIHLETPNYNVTRLRHDELNQMEPLPVSYRMMEQPA
                     EAEKAEAEGAEAKPVRLQAAVQGITPEQPAPMPVAAPPAAAPSILQKIFGWLTRKPEG
                     KPEQPSARPREVRRPHREPGRRDRREGQGGPRRPMPGEQPRHGAGQRRPERGPQPERG
                     MRGERGERPERPERPERPERPERGAQRDRAPQRERTQPAAVESAGRGEAASHADQRRD
                     RREPRGERPERPRHEGAPHAEGEGRGRRRRGRRDRGGREEADAGRTAPAASAREPVSP
                     AAAAPTPEPVAAEEPPQYRAPAPQPPPEPAREVAYVAPPRVETAAAAVAEPAESSAYR
                     ARPAPQPFSLPPDLVQIETTHHGYAAQEPLPAEESQARRPRRPRPEPAEPSEPLVQVE
                     TRQGE"
     gene            3251..>3527
                     /locus_tag="VD791_11065"
     CDS             3251..>3527
                     /locus_tag="VD791_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HXV08561.1"
                     /translation="MNSLSKAPERGPAPVTTAEVDQEAAGQRLDNFLVRRLKGVPKSH
                     VYRLLRTGQVRVNSRRVDATYRLQSGDRVRIPPVRTAQPAAARGVRGP"
BASE COUNT          422 a         1238 c         1254 g          613 t
ORIGIN      
        1 tggtcgagga agcgtcccgg ggcctgctcg ccagcctcct ggtcgagctg ccccgggtca
       61 ccggaaggga ctgacgcgtc tgcctggtgc gtcccaactg ggcgcgtccg cttgggcgcg
      121 tcctcttggg cgcgttactc gccctggcgc gtctccacct gcaccagcgg ctcgctcggc
      181 tcagccggct ccggccgcgg ccggcgcggg cggcgcgctt gcgactcctc ggcaggaagc
      241 ggctcctgtg cagcgtaccc gtggtgcgtg gtctcgatct ggaccaagtc cggcggaagc
      301 gagaacggct gtggtgcggg tcgggcgcgg taggcggaag actcggccgg ctcggccacg
      361 gcagcggcag ccgtttccac ccgcggtgga gcgacatacg ccacctccct tgccggttcg
      421 ggcggaggtt gtggggcagg cgcccggtac tgcgggggct cctcggccgc cacaggctcg
      481 ggtgtgggcg ctgcggcggc ggggctcacc ggctcgcggg cgctcgctgc aggcgccgtc
      541 cgaccggcgt ccgcctcttc gcggccaccg cggtcgcggc gaccgcgccg gcgccggcca
      601 cggccttccc cttcggcatg cggcgcgccc tcgtggcgcg gccgctcggg acgctcgccg
      661 cgcggctcgc ggcggtcgcg gcgctggtcc gcatgcgatg ccgcttctcc gcgcccggcc
      721 gattcgaccg ccgccggctg cgtgcgctcg cgttgcgggg cgcggtcgcg ctgcgcccca
      781 cgctccgggc gttcagggcg ctccgggcgt tcggggcgtt cggggcgctc gccgcgttcg
      841 ccgcgcatgc cgcgctcagg ctgcggtccg cgctcggggc ggcgctgccc ggcgccatgc
      901 cgtggctgct cgcccggcat cggccggcgc ggaccgccct ggccttcgcg ccgatcgcgg
      961 cggccgggct cgcggtgcgg gcggcgtacc tcgcgcggcc gggcgctggg ttgctccggc
     1021 tttccttccg gcttgcgcgt gagccagccg aagatctttt gcaggatgga cggcgcggcc
     1081 gcgggcggcg cggccaccgg catgggcgcg ggctgctcgg gcgtgatgcc ttgcacggcg
     1141 gcctgcagcc gcaccggctt ggcctccgcg ccttccgcct cggccttctc cgcctccgcc
     1201 ggctgctcca tcatccgata gctgaccggc agcggctcca tctggttcag ctcgtcgtgc
     1261 cgcaaccggg tgacgttgta gttcggcgtc tccagatgga tgttcgggat cagcaccacg
     1321 ttgaccttca agcgctgctc cagcgcgtga aactcggctc gtttctcgtt gagcaggaag
     1381 gtggccacgt ccaccggcac ctgcgcgtgc accgccgccg tgttctcctt catcgcctcc
     1441 tcctgcagga tgcgcaggat gtgcagggcc gaggactcga cgctgcggat gtggccgatg
     1501 ccgttgcagc gcgggcaggc catgtgcgtg gtttccccga gcgaggtctg cagccgctgg
     1561 cgcgacagct ccatcagtcc gaagcgcgag atcttgccca tctgcacgcg cgcgcggtcg
     1621 aagcgcagcc cgtcgcgcag gcggttctcc acctcgcgct ggttgcggct ctgctccatg
     1681 tcgatgaagt cgatcaccac cagcccgccc agatcgcgca ggcgcagctg gcgcgcgatc
     1741 tcgtcggcgg cctccaggtt ggtcctgagc gccgtctcct cgatgtccga accgcgcgtg
     1801 gcgcgcgccg aattcacatc gatcgccacc atcgcctcgg tatggtcgat gacgatggcg
     1861 ccacccgagg gcagcggcac ctggcgcgca taggcggtct cgatctgatg ctcgatctgg
     1921 aagcgcgaga acagcgggac gtcgtccttg tagagcttca cgcggttcac gttcccgggc
     1981 atcacgtgcg ccatgaactg cctggcctgc tcgtagatgc tctcggtatc gaccagcagc
     2041 tcgccgatgt cctggtggaa gtagtcgcgg atcgcgcgga tcaccaggct ggattcctgg
     2101 tagatcagga aggccccgct ctgctgccgc gaggcgttct cgatggcgcg ccacagctgc
     2161 agcaggtagt tcaagtccca ctgcagctct tccagggtgc ggcccaggcc ggcggtgcgg
     2221 ccgatgatgc tcatgccctg cggcacttcc agggccgcga tgaggtcgcg cagctcgttg
     2281 cgctcttcgc cctccacgcg gcgcgacaca ccgccgcccc gcgggttgtt gggcatcagg
     2341 accaggtagc gcccggccag gctgacgaag gtggtgagcg ccgcgccctt gctgccgcgc
     2401 tcgtccttgt ccacctgcac gatcagctct tgcccgtcgc gcatcacgtc ctggatgcgc
     2461 gcccgggcgg gatcgacgtc gctcaggaag taggcgcggc tgacttcctt gaacggcagg
     2521 aagccgtggc gctcactgcc gtagtcgacg aaggcggctt ccaggctggg ctcgacgcgg
     2581 gtgatgactc ctttgtagat gttgcctttg cgttgctctt tccctgcggt ttcgatatcg
     2641 aggtcgacta gcttctgccc gtcgacgatc gcgacccgga gctcttccgc ctgggtcgcg
     2701 ttgaacagca tgcgtttcat gtttttgccc ccgcgctcgg agccgcgggc agggcaaaca
     2761 tcggacgcgg cgtcgctcgc gcgacgcgcc ggcgcccccc ggttggataa ctattcggtc
     2821 ggtgcagcag tcataattga atgcctcgcg aacagcgttc ttgggcgttt gccttgccgg
     2881 tcccggagct aacagttcgt cgatccatcg cccggcgggc ccgcgccggg gacggtcaca
     2941 gccgctccgc gcatgggcgc ccgctcggga aaggtgcctc ggcctcgatc aggctcgcgc
     3001 agcccctcgg atcaggattt cgacacgccg ctaggcttca ggaggggatt tgttgctccg
     3061 tagttgagcg cgttatcagg tccgcatcgc tagtcggttc gggtcagcga agttaacccg
     3121 tcgtgatggg tgcaggccac gagcctgggg agccgcccgc acggctgcgg gtgtcgcggt
     3181 ctccgcgtcg ccggcccgtc ccgggcgttc cgcccgggac gtgcgctgcg aaggatttag
     3241 tataagcaaa atgaatagct taagcaaagc gcccgaacgc gggccggcgc cggtgacgac
     3301 ggccgaagtg gatcaggagg ctgccggcca gcggctcgac aacttcctgg tgcgccgcct
     3361 caagggcgtg cccaagagcc atgtgtatcg gctgctgcgc accggccagg tgcgcgtgaa
     3421 cagccggcgc gtggatgcga cctaccggct gcagtccggc gaccgcgtcc gcattccccc
     3481 ggtgcgcacc gcacagccgg ctgcggcccg gggggtgcgg gggccgg
//
LOCUS       DASPGE010000347        11750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_793558, whole genome shotgun sequence.
ACCESSION   DASPGE010000347 DASPGE010000000
VERSION     DASPGE010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11750
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793558"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VD791_11070"
     CDS             complement(<1..616)
                     /locus_tag="VD791_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXV08562.1"
                     /translation="MRLAENVMHFARVLRGAGLPVGPDRVIDALRALEIAGVGNREDF
                     YWTLASVFLDRREQFDVFDQAFHVFWRNPQLLERVMALLLPRVRGRAAEQPLSNRVAD
                     PLAPKPRRERDGRETPPEIQLDATLTFSERELLHKADFESMTVEELAQAKRLIAGLRL
                     PIPEVRTRRLRPDPHGARVDLRGTLRASVRGSADLIPLERRSPQR"
     gene            748..1260
                     /locus_tag="VD791_11075"
     CDS             748..1260
                     /locus_tag="VD791_11075"
                     /inference="COORDINATES: protein motif:HMM:NF022338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3093 family protein"
                     /protein_id="HXV08563.1"
                     /translation="MTETFRLAPMAPFIRWLTWLLWALPAGFAVAALASPRSVGLLWI
                     ALLLGVLYGAVWIWWRPGRFEVSARGLVLVFPGRRAAIPISDIESVSVVDPKAFRQQF
                     GLALRVGAGGLWGGFGWLWTSKGWVEFYVSALDRYVLIRRRRRIPLLISPEQPQRMAA
                     MLERQLPAPR"
     gene            1365..2213
                     /locus_tag="VD791_11080"
     CDS             1365..2213
                     /locus_tag="VD791_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXV08564.1"
                     /translation="MRTRRSAVLAVALLLLAGACSKKDESSPASEPVAAPVAGLVGSD
                     AAALAKSARFVAPDKLTVCSDIPYAPFEFNDRSGGDQLTGFDVELVRVLAAQLTLTAE
                     FKTTPFDAIIPSLAAGNCDMVASATTITEERKKKVAFTQPYFDADQALLVRAADQERY
                     PRLSGLKGKTIGVQSGTTGETYAKANKPAGATVKDFPGADDLFNALASGDIDAVLQDF
                     PVNKYRALKAPDQFAVTETFKTGEQYGFAVAMDNPNLVAALDAALASVRQSGDYDKTY
                     KAWFGD"
     gene            2311..3105
                     /locus_tag="VD791_11085"
     CDS             2311..3105
                     /locus_tag="VD791_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HXV08565.1"
                     /translation="MASTRRQRAALIRYAIYAVTLALLAWVALATDWARLQESFFDWQ
                     IFRSLFPDILLRAARNTLIFTFFGFSGGLAIGLALALMRLSPVRPYRWAAALYIEIFR
                     GLPALITIVLIGFVLPIALQVRVPGTYGPGSLALAIVTGAYLAETIRAGIEAVPKGQM
                     EAARSLGMSHARAMISIVIPQAFRVILPPLTNELVLLLKDTSLLFVLGTTDQTIELAK
                     FGRDAVADTFNGTPLVAVALTYLAITLPLTRAVAWLERRQKRAVRG"
     gene            3102..3851
                     /locus_tag="VD791_11090"
     CDS             3102..3851
                     /locus_tag="VD791_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HXV08566.1"
                     /translation="MNQAASPAIELIALEKRFGPLQVLKGVDLAIASGEVVCLIGPSG
                     SGKSTLLRCVNRLEQPTGGRILIDGENICDPDCDLDRVRTKIGMVFQHFNLFPHLSVL
                     DNLMIAQCKVLKRSRGEAQSKARTVLERVGLLDKIHEYPARLSGGQQQRVAIARALAM
                     DPEMMLFDEATSALDPELVGEVLNVMRALAEDGMTMMAVTHEMGFARRVADRVVFMDG
                     GVIVEAGHPERVLGDPQQERTRRFLQRVSDR"
     gene            3889..5136
                     /locus_tag="VD791_11095"
     CDS             3889..5136
                     /locus_tag="VD791_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008365064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HXV08567.1"
                     /translation="MKRFSILALARGALSGHRWPAYWRTPQPKPAYDVVIVGGGGHGL
                     ATAYYLAREHGISNVAVLEKGWLGGGNTGRNTTICRSNYLWDESAALYEHALRLFEGL
                     SRELNFNVMLSQRGVLNLAHNLHDVQEGRRRVYANRLNGIDAEWLEPPQVQRFCPILE
                     VSPHARYPILGGTLQRRAGTARHDAVAWGFARAADALGVDIIQGCEVTGFDIRNGRVE
                     GVRTTRGALRAGKVAIVAAGHNSVLCTLAGVRLPLQSHPLQALVSEPIKPVLDTVVMS
                     GAVHVYVSQSDKGELVLGAGIDAYNSYAQRGSFHVIESQMAALVELFPVFSRLRMMRT
                     WGGIVDVSPDASPIIGPLPVRGMFLNGGWGTGGFKATPGSGHVFAWTIAKDSPHPLAA
                     PFGLERFTTGALVDEHGATAVAH"
     gene            5144..5425
                     /locus_tag="VD791_11100"
     CDS             5144..5425
                     /locus_tag="VD791_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HXV08568.1"
                     /translation="MLLIPCPWCGPRDETEFRYGGQAHIARPAAPDELSDGEWAQYVF
                     MRDNPKGPFAERWNHACGCRRWFNAIRHTVTHEIVAVYKPGEPRPEQGG"
     gene            5422..8412
                     /locus_tag="VD791_11105"
     CDS             5422..8412
                     /locus_tag="VD791_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011354097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit alpha family protein"
                     /protein_id="HXV08569.1"
                     /translation="MTAFRLPSGGRIDRARTLRFDWNGRTFAGHPGDTLASALLASDV
                     LLLARSLKYHRPRGVLCAGSEEPNALVQLGTGAWSEPNTRATQIELVDGLAAAAQNCW
                     PGVELDFGAVSSWFSRLLVAGFYYKTFMWPRSFWTRVYEPLIRRAAGLGRAPPAADPD
                     LYDKMHAHVDVLVAGGGPAGLAAALAAARAGARVLIADEQNELGGGFLSLPGAGRAGP
                     AWVAAALAELYAAPEVRVLPRTTVFAYHDHNYLCLLERRTDHLAAAAPPDAARLRLWK
                     VRAKRAILATGAHERPIVFPGNDRPGVMLASAARTYLNRYAVAIGRRVVVFTNNDSAY
                     AAALDLARNGVHVTVVDVRAESEAGAAAAAAKAGVTVRFGSVICDIRGARRVRGVRVA
                     GLDGKGGHWLEADAVAMSGGWTPAVHLFSQSQGRLAWDEALASFVPAHSVQAQRCVGA
                     CRGVFSAHTALEEAARAGSEAARAAGFEAGAHVELPEVDDQPVRALAPLWRVERGASR
                     SKAFVDLQSDVTAADVDLAVREGFVAAEHLKRYTTTGMATDQGKTSNLNAAAILAALT
                     GKSIAETGTTTFRPPYTPVAFGALAGRDLGERLEPIRVTPLHRWHAEHEAVFENVGQW
                     KRPWYYPRPGEDLDAAVARECGTVRTAVGVLDASTLGKIEVTGPDAARFLDRVYTNTI
                     STLGAGRCRYGLMCREDGMVLDDGVSTRLDGHRFFMTTTTGGAARVLDWLEDLLQTEW
                     RDLKVYLTSVTEQWSVITVAGPRAREVLQRMAPRMALDNAAFPVLSMREGEVAGVAAR
                     VFRISFSGELSYEIHVRADDAPGVWEAVMRAGAPFGIAPYGTQAMHVLRAEKGFIVVG
                     HETDGSVTPLDLGLERMVSKTKDFIGRRSLARPDTTRADRKQLVGILPDDPRTVLPEG
                     AQLVAGELPRPHPRRNRAIPMIGHVTSSYWSASLARGFALALVESGRSRIGQSVWAPL
                     ETGAVRARIVEPAFWDPAGARQHA"
     gene            8405..8995
                     /locus_tag="VD791_11110"
     CDS             8405..8995
                     /locus_tag="VD791_11110"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HXV08570.1"
                     /translation="MPESALGRRADRLVAGSVPGAVTLAEIPFLAKIGLRGDPADARF
                     RDAARSALELDLALQPNTFTRRDALRCLWLGPDEWLVVGPAGAVVELSGRLEAALQGR
                     HAAIVDLSAAHAAFELRGERAREVLAKACSLDLHPRAFRPGQCAQSNFARTQAVLALE
                     DDAPAFRLFVRRSFALYLADWLLDAMREYRAVNRLS"
     gene            complement(9073..10827)
                     /locus_tag="VD791_11115"
     CDS             complement(9073..10827)
                     /locus_tag="VD791_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXV08571.1"
                     /translation="MSETELKQRLAAILAADAAGYSRLMRVDERATVAALDAARTVFR
                     SHIESNGGRVIDMAGDSVLAVFELATSAVCASLAIQRELDAQASAVPEDRRLPFRIGV
                     HLGEIIEKSDGTVYGDGVNIAARLQGLAEPGGITASESIRIAVLGRVSATFDDRGEQA
                     VKNIPDPVRAFSIRPDGDEQRPPAATGFDSPLADKPSIAVLPFANMSGDPEQDYFADG
                     ITEDIITQLARFRAICVIARNSVFVYKNKAVRVQDVGRDLGVGYVVEGSVRKAGARIR
                     VNVQLIDAVSATHVWAERYDRDLVDIFELQDELTQRIVAALPRRVETAHLEQLKRKKP
                     RDMGVYDCVLRAKLCHHRGTADDNALGLQLLDQAIRIDPDHAPAYGWRACTRAQAMVR
                     GYAEFSDAKEQEVLRDVERGLSIDDNDLECLRILCEFRIAQKRLDEALTFNDKLLRLN
                     PSDPRLLAQRGEILTWRGQAQEGIEWIERGMRLDPHEADAWAHLLGRAQFGAGRYTEA
                     IAAFRRVPSMRYGHHAYLAACYAQLDDRAAAADERNHVLRLKPDFRASDFCRSLSFAD
                     QAHCAHVRESLEKAGLPE"
     gene            10881..>11750
                     /locus_tag="VD791_11120"
     CDS             10881..>11750
                     /locus_tag="VD791_11120"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HXV08572.1"
                     /translation="MPVLFIIPSTRRVSFDLLRPPSVNGMTMALEARGLVFPGGILWL
                     THPRSTGRVTIDYDKLVNWQIPEVEEHLTRRDTILYALGVGLGADPCDAGELRFVYED
                     RLQALPTMAIVLGYPGPWHAHPDTGITRSRVVHGEQGFRIVKPLPVEGDIVGKTRVTG
                     LVDKGTDKGALLMTETTVRDKASGDVICTLTSTAFCRADGGFGGSGGPVRTPHPIPDA
                     PPQHVCDLPTLPQAALIYRLSGDYNPLHADPAYARQAGFDRPILHGRCTFGIAGHAIL
                     KTLCGYDPARFVSM"
BASE COUNT         1650 a         4077 c         4185 g         1838 t
ORIGIN      
        1 ggcgctgcgg cgagcggcgc tcgagcggga tcaggtccgc gctgccgcgc acgctggcgc
       61 gcagcgtgcc gcgcaggtcc acgcgcgctc cgtgcggatc gggccgcagg cgccgtgtgc
      121 gcacctcggg aatgggcaac cgcagtccgg cgatcagccg cttcgcctgc gccagctcct
      181 ccaccgtcat gctctcgaaa tcggccttgt gcagcagctc gcgttcggag aaggtgaggg
      241 tggcgtcgag ctggatctcc ggcggcgtct cgcggccgtc gcgctcgcgc cgcggcttgg
      301 gcgccagcgg atcggccacg cggttcgaca gcggctgctc ggctgcgcgc ccgcgcacgc
      361 gcggcagcag cagggccatg acgcgctcga gcagctgcgg gttgcgccag aacacgtgga
      421 acgcctgatc gaacacgtcg aactgctcgc gccggtcgag gaacacgctg gcgagcgtcc
      481 agtagaagtc ctcgcggttg cccacaccgg cgatctccag cgcgcgcagc gcgtcgatca
      541 cccggtccgg ccccaccggc aggccggccc cgcgcagcac gcgggcgaag tgcatcacgt
      601 tctcggcgag tctcatgcgc ggcggggagg gtgcgggcgg catccggctg gttgcaggcc
      661 caactatacc aacccgaggg gtgcctaagg gtgcgcggct ttcggacgat acttgatcat
      721 ccgagcccgg cacgcgtttg cgctgccatg acagagacct tccgcctggc gccgatggcg
      781 ccgttcatcc gctggctcac ctggctgctg tgggcgctgc cggcgggatt cgccgtggca
      841 gcgcttgcca gcccgcgcag cgtcggcctg ctctggattg cgctcttgct cggggtcctg
      901 tacggggcgg tatggatctg gtggcggccc ggtcgcttcg aggtctcggc gcggggcctt
      961 gtgttggtgt tcccaggccg gcgcgcggcg atcccgattt ccgacatcga gtccgtgagc
     1021 gtggtggatc cgaaagcgtt ccgccagcag ttcggactgg cgctgcgagt gggcgccggc
     1081 ggcctgtggg gcggcttcgg ctggttgtgg accagcaagg gctgggtgga gttctacgtc
     1141 tccgccctcg accgttacgt gttgatccgg cggcgccggc gcatcccgct gctgatctcc
     1201 ccggagcagc cgcaacgcat ggcggcgatg ctcgaacggc aactgcctgc tcctcgctga
     1261 cgaggttctc gattctcgcg ctcgcggtca ggcgcaggcc aggccaagcg cataccgccg
     1321 gctatggtac cgttcgcact tccggatcct gcacggaggt cccgatgcgc acgcgccgct
     1381 ctgccgtcct cgcagtggcc ctgctcctgc tggccggcgc ctgctcgaag aaggacgagt
     1441 cgtcgccggc ctccgagccg gtggccgccc cggtggccgg cctggtgggc agcgatgccg
     1501 cggcgctggc caagagcgct cggttcgtgg cgccggacaa gctcaccgtg tgctcggaca
     1561 tcccgtacgc gccgttcgag ttcaacgatc ggtccggcgg cgatcagctc accggcttcg
     1621 acgtcgagct ggtccgggtc ctggcggcgc agctcacgct caccgccgag ttcaagacca
     1681 cgccgttcga cgcgatcatc ccgtcgctcg ccgccggcaa ctgcgacatg gtcgcctcgg
     1741 ccaccaccat caccgaggag cgcaagaaga aggtggcctt cacgcagccg tacttcgacg
     1801 ccgatcaggc gctgctggtc cgcgctgcgg accaggaaag atacccgcgg ctctccggcc
     1861 tgaagggcaa gaccatcggc gtgcagtcgg gcaccaccgg cgagacttac gccaaggcca
     1921 acaagcccgc gggcgcgacg gtcaaggact ttcccggcgc cgacgacctg ttcaacgcgc
     1981 tcgcatcggg cgacatcgac gcggtgctgc aggacttccc ggtcaacaag taccgggcgc
     2041 tcaaggcccc cgatcagttc gccgtgaccg agaccttcaa gaccggcgag cagtacggct
     2101 tcgccgtggc gatggacaat ccgaacctgg tggcggcgct cgacgccgcg ctcgcgagcg
     2161 tgcgccagtc cggcgactac gacaagacct acaaggcgtg gttcggcgac tagtggactg
     2221 cagcagccga ttcgccggta cccctcaccc caaccctctc cccccagggg agagggagtg
     2281 tgctccttgt tggaccgtcg cagctcagtc gtggcatcga cccggcgcca gcgggcggcg
     2341 ctgatccgct acgcgatcta cgcggtcacc ctcgcgctgc tcgcgtgggt ggcgctggcg
     2401 accgattggg ccaggctgca ggagagcttc ttcgactggc agatcttccg cagcctgttc
     2461 cccgacatcc tgctgcgggc ggcgcgcaac accctgatct tcaccttctt cggcttcagc
     2521 ggcgggctcg ccatcgggct cgcgctcgcc ctgatgcgcc tgtcgccggt gcggccgtat
     2581 cgctgggcgg cggcgctcta catcgagatc ttccgcggcc tgcccgcgct catcaccatc
     2641 gtgctgatcg gcttcgtgct gccgatcgcg ctgcaggtgc gcgtgccggg cacctacggc
     2701 cccggcagcc tggcgctggc gatcgtcacc ggcgcctatc tggcggaaac catccgcgcc
     2761 ggcatcgagg cggtgcccaa gggccagatg gaggcggcgc gctcgctggg gatgagccac
     2821 gcgcgcgcca tgatctcgat cgtgatcccg caggcgttcc gcgtgatcct gccgccgctc
     2881 accaacgagc tggtgctgct gctgaaagac acgtcgctgc tgttcgtgct gggcaccacc
     2941 gaccagacca tcgagctggc gaaattcggc cgggatgcgg tcgccgacac gttcaacggc
     3001 acgcccctgg ttgcagtggc gctcacctac ctcgccatca cgctgccgct cacccgtgcc
     3061 gtggcctggc tcgagcgccg gcagaagcgt gcggtgcgcg gatgaaccag gccgcctcgc
     3121 cggcgatcga gctgatcgcg ctggagaagc gcttcggccc gctgcaggtg ctgaagggcg
     3181 tcgacctcgc cattgcctcc ggagaggtgg tgtgcctgat cgggccgtcc ggctccggca
     3241 agtcgacgct gctgcgctgt gtcaaccggc tcgagcaacc caccggcggg cgcatcctga
     3301 tcgacggcga gaacatctgc gatccggact gcgatctcga ccgggtgcgc accaagatcg
     3361 gcatggtgtt ccagcacttc aacctgtttc cgcacctgag cgtgctcgac aatctgatga
     3421 tcgcccagtg caaggtgctg aagcgcagcc gaggcgaagc gcagagcaag gcgcgcaccg
     3481 tgctcgagcg cgtggggctg ctggacaaga tccacgagta tccggcgcgc ctctccggcg
     3541 gccagcagca gcgcgtggcc atcgcgcgcg cgctcgccat ggatccggag atgatgctgt
     3601 tcgacgaggc cacctcggcg ctcgaccccg agctggtcgg cgaggtcctc aacgtcatgc
     3661 gcgcgctggc cgaggacggc atgaccatga tggcggtgac gcacgagatg ggtttcgcgc
     3721 gccgggtggc cgaccgggtc gtgttcatgg acggcggcgt gatcgtcgag gcggggcatc
     3781 ccgagcgggt gctcggcgac ccgcagcagg agcgcacacg ccgcttcctg cagcgggttt
     3841 ccgatcgctg aacggcgcgg caagcggccg cgtcggaacg ggggaggcgt gaagagattc
     3901 tcgattctgg cgctggcgcg cggggcgctg agcggtcacc gctggccggc gtactggcgc
     3961 acgccgcagc ccaagcccgc ctacgacgtc gtcatcgtcg gcggcggcgg ccacgggctc
     4021 gccaccgcct actatctggc cagggaacac ggcatctcca acgtggcggt gctggagaaa
     4081 ggttggctgg gcggcggcaa caccgggcgc aacaccacca tctgccgctc caactacctg
     4141 tgggacgagt cggcggcgct gtacgagcac gccttgcggc tgttcgaagg tttgtcgcgc
     4201 gagctcaact tcaacgtcat gctgtcgcag cgcggtgtgc tgaacctggc gcacaacctg
     4261 cacgacgtgc aggagggccg gcgccgggtg tacgccaacc gcctgaacgg catcgacgcg
     4321 gaatggctcg agccgccgca ggtgcagcgg ttttgtccga tcctggaagt ctcgccgcac
     4381 gcccgctatc cgattctcgg gggcacgctg cagcggcggg cggggaccgc gcggcacgac
     4441 gcggttgcat ggggtttcgc gcgcgcggcc gacgcgctgg gtgtggacat catccagggc
     4501 tgcgaggtca ccggcttcga catccgcaac gggcgcgtgg aaggcgtgcg caccacgcgc
     4561 ggggcgctgc gcgcgggcaa ggtcgccatc gtcgcggcgg gccacaactc ggtgctgtgc
     4621 acgctggcgg gcgtgcgcct gccgctgcag agtcatccgc tgcaggcgct ggtgtccgag
     4681 ccgatcaagc cggtcctcga caccgtggtc atgtccgggg ctgtgcacgt gtacgtgagc
     4741 cagtcggaca agggcgagct ggtgctcggc gccggcatcg acgcctacaa ctcctacgcg
     4801 cagcgcggct cgttccacgt gatcgagagc cagatggcgg cgctggtgga gctgtttccg
     4861 gtgttcagcc ggctgcgcat gatgcgcacc tggggcggca tcgtcgacgt cagccccgac
     4921 gcctcgccga tcatcggccc gctgcccgtg cgcggcatgt tcctgaacgg cggctggggc
     4981 accggcggct tcaaggccac gccaggctcg ggccacgtgt tcgcctggac catcgccaag
     5041 gactcgccgc atccgctggc ggcgccgttc ggcctggagc gcttcaccac cggggcgctg
     5101 gtagacgagc acggcgcgac ggcagtggcg cactgatgga cttatgctgc tgatcccctg
     5161 cccctggtgc gggccgcgcg atgagaccga gtttcgctac ggcggccagg cgcacatcgc
     5221 ccggcctgca gcgccggacg agttgtcgga cggcgaatgg gcgcagtacg tgttcatgcg
     5281 cgacaacccc aagggcccgt tcgccgagcg ctggaaccac gcctgcggtt gccggcgctg
     5341 gttcaacgcg atccgccaca cggtcacgca cgagatcgtc gcggtctata agcccggcga
     5401 gccccggccg gagcagggcg gatgacggcg ttcaggttgc cgtccggcgg tcgcatcgat
     5461 cgagcacgca cgctgcggtt cgactggaac ggccgcacct tcgcggggca ccccggggat
     5521 acgctcgcct ctgcgctgct cgccagcgac gtgctgctgc tcgcgcgcag cctcaagtat
     5581 caccgcccgc gcggcgtgct gtgcgcggga tccgaagagc ccaacgcgct ggtgcagctc
     5641 ggcaccgggg cgtggtcgga gccgaacacg cgcgccacgc agatcgagct ggtcgacggg
     5701 ctcgccgccg cggctcagaa ctgctggccg ggcgtggagc tggacttcgg cgcggtgagc
     5761 agctggttct cgcgcttgct ggtggccggc ttctactaca agaccttcat gtggccgcgc
     5821 agtttctgga cgcgcgtcta cgagccgctc atccgccgcg cggcggggct tggccgagcg
     5881 cccccggcag cggacccgga cctgtacgac aagatgcacg cgcacgtgga cgtgctggtg
     5941 gccggaggcg ggccggcggg cctcgcggcg gcgctggccg cggcgcgggc gggcgccagg
     6001 gtcctgatcg cggacgagca gaacgagctg ggcggcggct tcctgtcgct gccgggcgcg
     6061 ggccgcgccg gcccggcgtg ggtcgcggcg gcgctggccg agctgtacgc tgcgcccgag
     6121 gtgcgcgtgc tgccgcgcac gacggtgttc gcctaccacg accacaacta cctgtgcctg
     6181 ctcgagcggc gcaccgatca cctcgcagcc gccgcgccgc cggatgcggc gcggctgcgg
     6241 ctgtggaagg tgcgggcgaa gcgcgcgatc ctggccaccg gcgcgcacga gcggccgatc
     6301 gtgtttcccg gcaacgatcg gccaggcgtg atgctggcct ccgccgcgcg cacttatctg
     6361 aaccgctacg ccgtggcgat cggccggcgc gtggtggtgt tcaccaacaa cgattcggct
     6421 tatgccgctg cgctggacct cgcacgcaac ggcgtgcacg tcaccgtcgt ggacgtgcgt
     6481 gcggagtcgg aagcgggcgc ggcggccgcc gccgcgaagg cgggagtaac ggtgcgattt
     6541 ggcagcgtca tctgcgacat ccgcggagcc aggcgggtgc gtggcgtacg cgtggccggt
     6601 ctcgatggaa aaggcggcca ctggctggag gccgatgcgg tggccatgtc gggcggctgg
     6661 accccggccg tgcacctgtt ctcgcaatcg cagggccgcc tcgcctggga cgaggcgctc
     6721 gcgagcttcg ttcccgcgca ctccgtgcag gcgcagcgtt gcgtgggcgc gtgccgcggc
     6781 gtcttctcgg cgcacactgc gctcgaggaa gcggcccgcg ccggaagcga agcggcgcgc
     6841 gcggccggtt tcgaagccgg ggcgcacgtc gagttgcccg aggtcgacga tcaaccggtg
     6901 cgtgcgctcg cgccgctgtg gcgcgtcgag cgcggcgcgt cgcggtccaa ggcgttcgtg
     6961 gacctccaga gcgacgtcac cgccgccgac gtggatctgg cggtgcgcga aggcttcgtg
     7021 gccgcggagc acctgaagcg ctacaccacc acgggcatgg ccaccgatca gggcaagacc
     7081 tcgaacctca acgcggcggc gatcctggcg gcgctcacgg gcaagtcgat cgccgagacc
     7141 gggaccacca ccttccgccc gccctatacc ccggtcgcat tcggcgctct ggcgggccgc
     7201 gatctgggcg aacgactcga accgatccgc gtcacgccgc tgcaccgctg gcatgccgag
     7261 catgaggcgg tgttcgagaa cgtgggccag tggaaacgcc cgtggtacta cccgaggccg
     7321 ggcgaagacc tggacgccgc ggtcgcgcgc gaatgcggca ccgtacgcac ggcggtgggc
     7381 gtgctggatg cgtccaccct gggcaagatc gaggtgaccg gtccggatgc ggcgcgcttt
     7441 ctcgaccgcg tgtacaccaa caccatcagt acgctcggcg cgggccgttg ccgctacggg
     7501 ctgatgtgcc gggaagacgg catggtgctc gacgacggtg tgagcacgcg gctggacggg
     7561 catcgtttct tcatgaccac caccaccggc ggcgcggcgc gcgtgctcga ttggctcgag
     7621 gacttgctgc agaccgagtg gcgcgatctg aaggtgtacc tgacttcggt gaccgagcag
     7681 tggtccgtga tcactgtggc cgggccgcgg gcgcgcgagg tgctgcagcg gatggcgccg
     7741 cggatggcgc tcgacaacgc ggcctttccc gtcctgtcga tgcgcgaggg cgaggtggcc
     7801 ggggttgcgg cacgcgtgtt ccggatcagc ttcagcggcg agctgagcta cgagatccac
     7861 gtgcgcgcgg acgacgcgcc gggcgtgtgg gaggcggtga tgcgcgccgg tgcgccgttc
     7921 ggtatcgccc cctacggaac gcaggcgatg cacgtgctgc gggccgagaa gggtttcatc
     7981 gtggtcggcc acgagaccga cggcagcgtg acgccgctcg acctcggcct cgagcggatg
     8041 gtgtcgaaga ccaaggactt catcggccgc cgctccctgg cgcggccgga tacgacccgg
     8101 gcagatcgca agcagctggt cggcatcctg ccggacgatc cgcgcacggt gctgcccgaa
     8161 ggcgcgcagc tggtcgcggg cgagctgccg cggccgcacc cgcggcgcaa ccgcgcgatc
     8221 ccgatgatcg ggcatgtcac gtcgagctac tggagcgcca gcctcgcgcg cggcttcgcg
     8281 ctcgcgctgg tggagtcggg gcgttcgcgc atcggccaat cggtctgggc gccgctggag
     8341 accggcgcgg tgcgcgcgcg catcgtcgag ccggcgttct gggacccggc gggggcgcgc
     8401 cagcatgcct gagagcgcgc tcgggcgccg ggccgaccgg ctggtcgcag gcagcgtgcc
     8461 gggcgccgtc acgctggcgg agatcccctt cctggcgaag atcggcctgc gcggcgatcc
     8521 ggccgacgcg cgcttccgcg acgcggcgcg cagcgccctc gagctggatc tggcgctgca
     8581 gcccaacacc ttcacccgtc gcgatgcgct gcgctgcctg tggctcggcc ccgacgagtg
     8641 gctggtagtc ggcccggccg gtgccgtggt cgagctgagc gggcgcctcg aggcggccct
     8701 gcagggtcgg cacgcggcga tcgtcgacct cagcgccgcc cacgctgcgt tcgagctgcg
     8761 cggcgagcgc gcacgggagg tgctggcgaa ggcgtgctcc ctggatctgc atccgcgcgc
     8821 cttccgcccg gggcaatgcg cgcaatcgaa cttcgcccgc acccaggccg tgctcgcgct
     8881 cgaggacgac gcgcccgcgt tccggctctt cgtgcggcgc tcgttcgcgc tctacctcgc
     8941 ggactggttg ctcgatgcga tgcgcgagta tcgcgctgtg aaccggttgt cctagctggt
     9001 ttcgcgcgaa tcgcctggat cgggcagctc gcccggcacg cctcgcgggg gcgcgccggc
     9061 cgcgcgggcg cctcattcgg gcaggccggc tttctcgagg ctctcgcgga cgtgggcgca
     9121 atgggcctga tcggcaaacg acagcgaacg gcagaaatcg cttgcccgaa aatccggctt
     9181 caggcgcagc acgtggttgc gttcgtccgc cgccgccgcg cgatcatcga gctgcgcgta
     9241 gcaggccgcc aggtacgcgt gatggccgta gcgcatcgaa ggcacccgcc tgaacgccgc
     9301 gatcgcctcg gtgtatcggc ccgccccgaa ctgcgcccgc ccgagcagat gcgcccaggc
     9361 gtcggcctcg tgcgggtcga gccgcatgcc ccgttcgatc cactcgatgc cttcctgcgc
     9421 ttgaccgcgc caagtgagga tctcgccgcg ctgcgcgagc aaccgcgggt cgctcggatt
     9481 gagccgcagc agcttgtcgt tgaaggtcaa ggcttcgtcc aggcgcttct gcgcgatgcg
     9541 gaactcgcac aggatgcgca ggcactcgag gtcgttgtcg tcgatcgaca ggcctctctc
     9601 cacgtcccgg aggacctctt gctctttcgc gtccgagaac tcggcgtagc cgcgtaccat
     9661 ggcctgcgcg cgcgtgcagg cacgccagcc gtaggccggg gcgtgatccg ggtcgatccg
     9721 gattgcctgg tcgagcagct gcagtcccag cgcgttgtcg tcggccgttc cgcgatggtg
     9781 acagagcttc gcccgaagaa cgcaatcgta cacgcccatg tcgcgcggct tcttgcgctt
     9841 gagctgctcg agatgcgcgg tttcgacgcg gcgcggcagg gcggcgacga tgcgctgcgt
     9901 caactcgtcc tgcaactcga agatatcgac gagatcgcgg tcgtagcgct cggcccagac
     9961 atgcgtcgcg ctgaccgcgt cgatcagctg gacgttgacg cggatgcgag cgccggcctt
    10021 gcgcacgctg ccctcgacca cgtaaccgac ccccaggtcg cggccgacat cctgtacgcg
    10081 caccgctttg ttcttgtata cgaacacgga attgcgggcg atcacgcaga tcgcccggaa
    10141 gcgcgcgagc tgggtgatga tgtcctcggt gatgccgtcg gcgaagtaat cctgctccgg
    10201 atctccgctc atgttggcga acgggagcac ggcgatcgag ggcttgtcgg ccagcggaga
    10261 gtcgaacccc gtggcagccg gaggccgctg ctcgtcgccg tcgggccgga tgctgaacgc
    10321 acgcaccggg tcggggatgt tcttgacggc ctgctcgccc cgatcgtcga aggttgcgct
    10381 caccctgccg agcaccgcga tgcggatcga ttcggaggcc gtgatgccgc ccggctcggc
    10441 gaggccctgc agccgggccg cgatgttgac gccgtcgccg tacaccgtgc cgtcggactt
    10501 ttcgatgatc tcgccgaggt gaacgccgat gcgaaagggc aggcgccggt cctccggcac
    10561 agcgctcgcc tgcgcgtcga gctcgcgctg aatcgccagg gacgcgcaca ccgcgctggt
    10621 cgcgagctcg aaaacggcga gcaccgagtc gcccgccatg tcgatcaccc gtccgccgtt
    10681 cgactcgatg tggctgcgga agacggtgcg cgccgcatcg agcgccgcga ccgtcgcgcg
    10741 ctcgtccaca cgcatcagac gcgaataacc ggccgcatct gccgccagga tcgccgccag
    10801 tctctgcttc agttccgtct cgctcacctg cgcccgccga ccatcgtcct cgagatccga
    10861 ttccttcggt cgatcggctc gtgcctgtgc ttttcataat accatccacc cggcgggtat
    10921 ctttcgacct gctgcgcccg ccgtcggtga acggcatgac catggccctg gaggcccggg
    10981 ggctggtgtt cccgggtggt atcctgtggc tcacgcaccc caggagcacc ggtcgcgtga
    11041 cgatcgacta cgacaagctg gtcaactggc agattcccga ggtcgaggag catctcaccc
    11101 ggcgcgacac catcctgtac gcgctcggcg tcgggctcgg cgccgacccc tgcgatgccg
    11161 gtgagctgcg cttcgtctac gaggaccggc tgcaggcgct gccgacgatg gcgatcgtgc
    11221 tgggctatcc cggcccctgg cacgcgcatc ccgataccgg catcacgcgc agccgcgtgg
    11281 tgcacggcga gcaggggttc cgcatcgtga agccgctgcc ggtggaaggc gacatcgtcg
    11341 gcaagacgcg tgtgaccggg ctggtggaca agggcacgga caagggcgcg ctgctgatga
    11401 ccgagaccac ggtacgcgac aaggcgagcg gcgatgtcat ctgcacgctc acttccaccg
    11461 ccttctgccg ggcggacgga ggcttcggcg gatccggcgg cccggttcgc acgccgcacc
    11521 cgattcccga cgcgccgccg cagcacgtgt gcgatcttcc gaccctgccc caggcggcgc
    11581 tgatctaccg cctgtcgggg gactacaatc cgctgcacgc cgaccccgcg tacgcgcgcc
    11641 aggcgggttt cgacagaccg atcctgcacg gtcgctgcac gttcggcatc gccgggcacg
    11701 cgatcctgaa gacgctgtgc ggctacgacc ccgcgcgctt cgtctccatg
//
LOCUS       DASPGE010000348        18566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_91278, whole genome shotgun sequence.
ACCESSION   DASPGE010000348 DASPGE010000000
VERSION     DASPGE010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18566
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91278"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VD791_11125"
     CDS             complement(<1..224)
                     /locus_tag="VD791_11125"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401443.1"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HXV08573.1"
                     /translation="MKRYTASVGFDRRLAPFDIQGSLAHARMLHAVGVLSAKDLADIE
                     RGMAQIGADITAGRFDWSLDLEDVHLNIEKR"
     gene            453..1469
                     /locus_tag="VD791_11130"
     CDS             453..1469
                     /locus_tag="VD791_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXV08574.1"
                     /translation="MPSINQSLHRDALPEFRNLGVTLRIVFIVVFLCAFAALVRAASW
                     ATLFDAFLEVLAPVQPVLVLSLLALWALDGWLRRLEYPAGILAVFAVELIIVALVHQI
                     GQRFFPADSYGPLERHWLVTVAATALVLYYFHLRSRALSPALSEARLQALQARIRPHF
                     LFNSINAVLSLIRQEPKRAEAALEDLADLFRVLMADNRKLSTLEREVELTRQYLSLEQ
                     LRLGERLRVDWHIDKMPGEALIPPLVLQPLVENAVYHGIEPSVEPGVIDINAYKSRNE
                     VHLVLRNPYRRDGSHHSGNKMALANIRERLSLHFDAEAGLRTTVTDNAYQVHIVMPYR
                     TEAR"
     gene            1504..2274
                     /locus_tag="VD791_11135"
     CDS             1504..2274
                     /locus_tag="VD791_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HXV08575.1"
                     /translation="MNRHAQPLRVVIVDDEAPARSRIQDLLEDCAATLALEVVGEAAN
                     GRELLDLVQKRASDVVLLDIRMPEMDGIEAAQHLQKLAEPPSVIFTTAYDSYALKAFE
                     LHAVDYLVKPIRLRRLHEALSRVRAITPLSLEVLRELHPEPRKHLSVQERGRVILVPV
                     DKILYMRAELKYVTIRTAEREHLLEESLTRLEQEFRERFVRIHRSCLVARDCIDRFER
                     GAGDEGAEGGWNVVLRGLEERLPVSRRQQHVIKDFGRL"
     gene            2271..2804
                     /locus_tag="VD791_11140"
     CDS             2271..2804
                     /locus_tag="VD791_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08576.1"
                     /translation="MKALRALVPAALALAAAALGAAAQDAWETLGPGAIGAGRAELET
                     RLALHCQADTCRPAAGTADRFAGVPVERIDLAFDADRLARVTVRFDTQHYERVREAVR
                     DRFGEGADHSYLARAGMAGEFAAGVFVWSGPGLALVLEQYAGKITRSQLVYGTPRALA
                     ALVRDKSATPRGARRDL"
     gene            2866..3873
                     /gene="hemC"
                     /locus_tag="VD791_11145"
     CDS             2866..3873
                     /gene="hemC"
                     /locus_tag="VD791_11145"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HXV08577.1"
                     /translation="MPGDVAAGAWYPVTPSRGRCVPAVSVPAALTIATRESALALWQA
                     HHIRDRLAALHPGVRIAILGVTTEGDRRLDASLAKIGGKGLFVKELESALADDRADLA
                     VHSMKDVPMQLPPGFAIAAITAREDPRDAFVSLRYRQLGELPPGAKVGTSSLRRESQI
                     RARYPQLDVRPLRGNVDTRLRKLDQGQYDAIVLAAAGLKRLGLAGRITAILGADESLP
                     AAGQGALGIECRSERADLLALLRPLDDAATAWCVRAERAVSRALAGSCVVPIGAYAEL
                     GAGVLRLRGFVASPDGARMARAELAVPSADADPEAAGRRLADQLVAAGAREILAALPR
                     P"
     gene            3881..4642
                     /locus_tag="VD791_11150"
     CDS             3881..4642
                     /locus_tag="VD791_11150"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HXV08578.1"
                     /translation="MQPPLAGKAILIARPRRPAQALAERIRAAGGEALVFPVLEIDPV
                     APDEQGRAALAELGAAHLAVFVSVSAVEHGLALVRAAGGWPAGLGAAAVGSATASALR
                     AQGIERVLVPDQGGDSEALLALPELLDVSGRRIVIFRGMGGRELLATTLRSRGAQVAY
                     VECYRRGKPALDAGPVIERLHRGALHAVVAASGEALVNLVELLPAPALRALPLAVTHP
                     NVARAAHDLGFRRVQVALGEAHLMDALIAAICHDS"
     gene            4812..5798
                     /locus_tag="VD791_11155"
     CDS             4812..5798
                     /locus_tag="VD791_11155"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HXV08579.1"
                     /translation="MRQRINLRVVAVAVGLLLLAGLWLDTRGRINTLQQELVRELAEA
                     GSLGKESRQLAAQASQTGRDLEFKIGLLESRLAETQNQRLALEALYLELSRSRDERVL
                     AEVEQIVLSGSQQLQLAGNVKAALVALESADSRLQRSDSAQFAALRRTIARDIERLKS
                     APFVDMVGMSLRLDTLAHQAEQFGLAMYERPAQPPPPTPRPEGGGLARLAREAWQDLT
                     GLIRIQRLDSREVPLVAPSQEFFLRENLRMRLLSARIALLARDETAFKADVRSARDWL
                     QRYFDHGDKQVDAALSALKRLAETDVSIDLPDISASLDAVRQHKLARERGLR"
     gene            5808..7016
                     /locus_tag="VD791_11160"
     CDS             5808..7016
                     /locus_tag="VD791_11160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HXV08580.1"
                     /translation="MRLLTWIIGLFALAVAVTVAARSSAGYVVLTLPSHRVELSVNLA
                     VLLLLGVFGLLYLVMRTVILATGMPARAREYRRSQERLRARRMSEDALRAYLEGRYGR
                     ADRAARQAAELDEWPALNLALAARSAHELRNYAARDACLQQMETRAPQESYLRETTRA
                     DLLLQERRYLDALHSLSRLQDKHTGALRLELKAHQLAGNWEQVLALLPQLEKRKVLEP
                     PVLEQFRRYAVTENLKRKALDGKSLREYWDRLPAAERADARVAAAAAQSFIALGACAE
                     GHRIIEESLAQTWDAALLMLYVECLPRDARKHLERAEEWLKQHPRDPVLLLALGLVCM
                     HQALWGKARSYLEASLAIELSHTGYVKLGELLERMGKPEEASQVYRRGLELALEQLKQ
                     CTGGRRRPIL"
     gene            complement(7057..8076)
                     /locus_tag="VD791_11165"
     CDS             complement(7057..8076)
                     /locus_tag="VD791_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HXV08581.1"
                     /translation="MAFQITIRPSNRRFTAEPGETVLAAAMREGITLPYGCRNGACGS
                     CKGKLLEGQVDYGQYTAHILPDYEKKLGFALFCQAKPLSDLVIEARIISATGEIPIKK
                     LPCRVQRIERPAADVAVLYLTLPANERLQFLSGQYLEIILRDGKRRAYSMANPPEDDA
                     HLELHVRNMPGGAFTDYVFTRMKEKDILRFEGPLGTFFLREDSDKPMLFVASGTGFAP
                     IKSILLHAFHRGAGRQMVLYWGGRRPSDLYMSDLCRQWQAQHGNFSFVPVVSDALPED
                     GWQGRTGFVHRAVMEDFPDLSGYQVYACGAPIVVESAHKDFTSQCGLPEEEFFSDAFT
                     PAPAS"
     gene            8197..8529
                     /gene="cutA"
                     /locus_tag="VD791_11170"
     CDS             8197..8529
                     /gene="cutA"
                     /locus_tag="VD791_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HXV08582.1"
                     /translation="MEPVLLVLSNLPDRERALELARALVDGGLAACVNVLSGCTSIYR
                     WRGAVETADEVPVLIKTRQSLYPRVEQAIRRLHPYEVPEVIAVPLAAGLPAYLEWVCA
                     ETEPTDAR"
     gene            8526..10466
                     /gene="dsbD"
                     /locus_tag="VD791_11175"
     CDS             8526..10466
                     /gene="dsbD"
                     /locus_tag="VD791_11175"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HXV08583.1"
                     /translation="MRTPPAMRSGHAAPRRACARIRLAMLCAWLAAPCAPWAQQPLAR
                     SEAAVESASTEGGVLSRLRQFAGAQADAEPEFLPPDQAFKVRVTVRDAHTLAAEFVPA
                     QGYYLYRDKIGFTLPEGGPVAVAGVALPPGERKDDPNFGLTEVYHSPVRARVALARAD
                     SAEQPIALEVRYQGCADQGLCYPPEKKVFNLLLAAADPGAAPSPPVSDVPADESSLAA
                     GILSAGDFWAVVASFFGFGLLLSFTPCVFPMIPILSGIIVGRGHQVTRQHALALSGVY
                     VLGMAVTYALAGVAAGLTGTLLSNALQNPWVLGTFAALFVVLALAMFGFYELQLPAAL
                     QSRLSNASNRLKGGTLWGVFAMGVLSALIVGPCVAAPLAGALLYINQSRDALLGGSAL
                     FAMALGMGAPMIAVGVSAGALLPKAGAWMQTVKSFFGVVLLGLAIWIVSPVIPSVAHM
                     LLWAALLIVSAIYLHAIDPLPHNASGFRKLWKGVGLIALLLGVALLVGALSGTRDILQ
                     PLSGLRVAGGGASAASAEQPRFILVRSVEELERELAQAGGRPAMLDFYADWCISCKEM
                     ERFTFTDPRVRSRMDRMLLLKADVTASTPEDLALLKRFGLFGPPGTLFFGPGGEQLPH
                     RVIGFQDAERFAASLDAVLRAS"
     gene            10463..10999
                     /locus_tag="VD791_11180"
     CDS             10463..10999
                     /locus_tag="VD791_11180"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXV08584.1"
                     /translation="MSRAAQAWLLGAVAVAALVAGALLRPAPRGPAPAPVPEGAGSIL
                     LQTVLPDLTGTPQRVDQWKGKVLVVNFWATWCAPCRDEIPALVRLQREMGGQGLQVIG
                     IAIDQLEKVRPYAAEMGMNYPILIGELDAIDLARQAGNELGALPFTVVLDRNGNAVRA
                     ELGRVTEAKLAALVRPLL"
     gene            11262..11753
                     /gene="aroQ"
                     /locus_tag="VD791_11185"
     CDS             11262..11753
                     /gene="aroQ"
                     /locus_tag="VD791_11185"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HXV08585.1"
                     /translation="MATQQKPGREGPRRILVLHGPNLNLLGTREPSIYGAATLDEINA
                     RLTARAEADGAVLVCFQSNAEVALIDRVQQAASDGTQFIIVNPAAFTHTSVALRDALA
                     AVKLPFIEVHLSNVFAREPFRHHSYFTALAVGMICGLGARGYELALEYALGDYALGAT
                     KRS"
     gene            11757..12212
                     /gene="accB"
                     /locus_tag="VD791_11190"
     CDS             11757..12212
                     /gene="accB"
                     /locus_tag="VD791_11190"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HXV08586.1"
                     /translation="MDLRKLKKLIDLVQESGIAELEVTEGEEKVRISRVGQTAQAIVP
                     VAGGAAATPLPAAAQPAPAALAAAAEEKVEGHVVKSPMVGTFYRSPSPGAKTFVEVGD
                     TVAPGDTLCIIEAMKLLNEIEADHGGTIKEVYVENGQPVEYGEPLFLIV"
     gene            12343..13698
                     /gene="accC"
                     /locus_tag="VD791_11195"
     CDS             12343..13698
                     /gene="accC"
                     /locus_tag="VD791_11195"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HXV08587.1"
                     /translation="MFEKILIANRGEIALRIQRACRELGVKTVAVHSEADSDAKYVRL
                     ADESVCIGPAGSGHSYLNIPAIISAAEVTDAEAIHPGYGFLSENADFAERVEKSGFVF
                     IGPRPETIRLMGDKVSAKQAMIKASVPVVPGSDGALPEAPEAIIKLARGVGYPVIVKA
                     AGGGGGRGMRVVHTEAALVNAVTMTRAEAQAAFGNPTVYLEKFLENPRHVEIQVLADE
                     HRNAVYLGDRDCSMQRRHQKIIEEAPAPLINARLKARIGERCAEACRKIGYRGAGTFE
                     FLFEKDEFYFIEMNTRVQVEHPVTEMITGIDIVQAQIRVAAGQKLPFKQRDIQFRGHA
                     IECRINAEHPYKFTPSPGRISAFHTPGGPGIRVDSHVYTNYFVPPHYDSLIAKVISWG
                     DTREQAIAKMRIALSEMIVEGIQTNLPLHQELMLDSSFARGGTSIHYLEEKLAQQSRP
                     D"
     gene            13701..14594
                     /gene="prmA"
                     /locus_tag="VD791_11200"
     CDS             13701..14594
                     /gene="prmA"
                     /locus_tag="VD791_11200"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HXV08588.1"
                     /translation="MSWLRASFRIAPAAAEALADALLERGALAVELADADEGTPAEQA
                     IFGEPGAEPLPLWRHQSISALFPRDADPEAAIRAAVAALGLEAEPSVTLQPVAERDWV
                     RASQQQFAPIEVAPRLWIVPSWQAAPDPSAVNLILDPGLAFGTGSHPTTGQCLRWLHA
                     NLASGASVLDYGCGSGVLAIAAKRLGAGRVVAVDIDPVALEVTRANAATNTAEILVAS
                     PDAVPPGPYDVVLANILAGPLELLAPLLASHAPAGGRIVLAGILTGQAADVAAAYAPW
                     CDLVQVSEKDGWACLAGARKG"
     gene            14626..15531
                     /locus_tag="VD791_11205"
     CDS             14626..15531
                     /locus_tag="VD791_11205"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HXV08589.1"
                     /translation="MYTRCPQCDTVFRVTPQQLQVSSGQVRCGRCRAVFDAFSSLSSH
                     PPGREPGVAAEPAAESAFRPPAAHPAGDEALAAAWRARPVSPMPDPLFSTGSVLPEPS
                     APRAEAAAPRALREETRPAVAAQEPLTLPEELFPPGLTTPRRRWLWIGVSALLVLGLA
                     AQMAWFFPGQVLMRAPALQPALQQLCAALGCQVRLPRVPEQLFIESSDLQLLDAARPS
                     QVLLTATIRNRAGFAQDFPLLELTLTGDDSRTAARRVFHPGEYLDAAVERGRGIGAQQ
                     EVAIHLYLDTADLRATGYRLYLFFA"
     gene            15554..16651
                     /gene="gcvT"
                     /locus_tag="VD791_11210"
     CDS             15554..16651
                     /gene="gcvT"
                     /locus_tag="VD791_11210"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014906081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HXV08590.1"
                     /translation="MNPPELKRTPLFDAHRRAHARLTDFGGWEMPLHYGSQIEEHHRV
                     RRDAGVFDVSHMLAIDVAGGEARTFLGRLLANDVAKLAVPGKALYSCMLNEDGGVIDD
                     LIVYWRGEDRFRMVVNAGTADKDLAWIARQRDRLAPGVVLQPRRDLAVLAVQGPQARE
                     RVWQVRPALRAESEALAAFQAREQGPLFVARTGYTGEDGFEVMVPATDAEALWNDLLG
                     AGVAPCGLGARDTLRLEAGMNLYGQDMDESTTPFEAGLRWTVAMTGTRDFIGRAALAR
                     RAPRWNLLGLVLRERGVLRGHQPVRGALGPGEVTSGTFSPTLGRSIALARLPAAATPG
                     EMVEVQVRDRWLPAQVVNYPFVRRGKSLLPD"
     gene            16675..17064
                     /gene="gcvH"
                     /locus_tag="VD791_11215"
     CDS             16675..17064
                     /gene="gcvH"
                     /locus_tag="VD791_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HXV08591.1"
                     /translation="MTVPADLKYADSHEWARLEADGTVSVGITDHAQDRLGDLVFVEA
                     PKVGQTVAKGKECGVVESVKAASDLYAPVSGEVVAVNQELAGSPEKVNQNAYAAWMFR
                     VRPSNPAELNALLDAAAYEKVVAADQH"
     gene            17111..18469
                     /gene="gcvPA"
                     /locus_tag="VD791_11220"
     CDS             17111..18469
                     /gene="gcvPA"
                     /locus_tag="VD791_11220"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HXV08592.1"
                     /translation="MPFIPHTDDDVRAMLASIGVPHIESLFDEIPQTLRAARLTAVPP
                     QLGEMDVMRLMQERAERDGRWLNFVGAGAYEHHIPAAVWQIVTRGEFYSAYTPYQAEA
                     SQGTLQVLYEYQSMMASLTGLDATNASLYDGASALAEAILMAVRLHKSSRRVLVPASV
                     HPTYVKVARTIVSSQDIELIELPYCTEQGTTLPESLQRFGEKAFAALVIPQPNFFGAL
                     EQVHALTDWAHAQAALVIGVVNPVALALLVPPGRWGKRGADIAVGEGQPLGVPLANGG
                     PYFGFLACKQAHVRQMPGRVAGRTVDLDGQPGFTLTLQAREQHIRRSKATSNICTNQG
                     LAVTAATIHMALLGPQGLQRVAQACHANLHLLLGQLERETGVRRRFAAPVFHEAVIAL
                     GAPAAPLLERMRKQRVLGGLAVGGDYPELTDSILVCATETKTPGDLDRYVEALIAARR
                     AG"
BASE COUNT         2719 a         6371 c         6606 g         2870 t
ORIGIN      
        1 cgcttctcga tgttgagatg cacgtcctcc aggtccagcg accagtcgaa gcggccggcg
       61 gtgatgtcgg cgccgatctg tgccatcccg cgctcgatat cggccaggtc cttggcggac
      121 aggacgccga cggcgtgcag catgcgggca tgggcgagcg aaccctggat atcgaacggc
      181 gccagccgcc ggtcgaagcc caccgaggcg gtgtagcgtt tcaccaggtc gtccaccggt
      241 tcggtgaacc gcccggacca cgctgccgag ggcggggcgg acgaagccgg cgcggccggg
      301 gcgccggccg ggagcggatt gggggttggt tttttcatcg gcgacggggg gcggccggca
      361 gcgcggcgcg ggctgtcatc tggcatggcc tgtgctggaa agggcccgcc acgtcatgga
      421 cttgcgcccg accggcctga aagtggcgat cgatgccgag tataaatcaa agcctgcacc
      481 gcgacgccct gcccgagttt cgcaacctgg gggtgacgct gcgcatcgtg ttcatcgtcg
      541 tgttcctgtg cgccttcgcc gccctggtgc gcgccgcctc gtgggcgaca ctcttcgacg
      601 ctttcctgga ggtgctggcg ccggtgcagc cggtgctggt gctgagcctg ctcgcgctgt
      661 gggccctgga cgggtggctg cggcgactcg agtacccggc cggcatcctg gcggtgttcg
      721 cggtcgaact gatcatcgtc gcgctcgtcc accagatcgg gcaacgcttc ttcccggccg
      781 attcctacgg gccgctggag cgccactggc tggtcaccgt ggccgccacc gcgctggtgc
      841 tgtattactt ccatctgcgc agccgcgcct tgtcgccggc gctgagcgag gcgcggctgc
      901 aggccctgca ggcgcgcatc cgcccgcatt tcctgttcaa cagcatcaac gcggtgctga
      961 gcctgatccg gcaggagccc aagcgcgccg aggcggcgct cgaggacctg gccgacctgt
     1021 tccgcgtcct gatggcggac aaccgcaagt tgtccaccct ggagcgcgag gtcgagctca
     1081 cgcgccagta tctgagcctc gagcaattgc gcctcggcga gcggctacgc gtggactggc
     1141 acatcgacaa gatgcccggc gaggccctga tcccgccgct ggtgctgcag ccgctggtgg
     1201 aaaacgccgt gtaccacggc atcgagccct cggtcgaacc gggcgtgatc gacatcaacg
     1261 cctacaagtc gcgcaacgaa gtgcacctgg tgctgcgcaa cccttaccgc cgcgacggca
     1321 gccaccactc cggcaacaag atggcgctcg ccaacatccg cgagcggctg agcctgcact
     1381 tcgacgccga ggcggggctg cgcaccaccg tgacggacaa cgcctatcag gtgcacatcg
     1441 tcatgccgta tcggaccgag gcgcgctgat aaagcaggca ttcttgatgc atcgaggaca
     1501 accatgaaca gacacgccca gccgctgcgg gtggtgatcg tcgacgacga ggcaccggcc
     1561 cgcagccgca tccaggatct gctcgaggat tgcgccgcca cgctcgcgct ggaggtggtg
     1621 ggcgaggccg ccaacggccg cgagctgctc gatctggtgc agaagcgggc cagcgacgtg
     1681 gtcctgctcg acatccgcat gccggagatg gacggcatcg aagcggcgca gcatctgcag
     1741 aagctggccg aaccgccctc ggtgatcttc accaccgcct acgacagcta cgcgctcaag
     1801 gccttcgagc tgcatgcggt agactacctg gtcaagccga tccggctgcg gcgcctgcac
     1861 gaggccctgt cgcgggtgcg cgcgatcacg ccgctgtcgc tggaggtgct gcgcgagctg
     1921 caccccgagc cgcgcaagca cctgagcgtg caggagcgcg ggcgcgtgat cctggtgccg
     1981 gtggacaaga tcctgtacat gcgcgccgag ctcaagtacg tcaccattcg caccgcggag
     2041 cgcgagcatc tgctggagga gtccctgacg cgactggagc aggagttccg cgagcgcttc
     2101 gtgcgcattc accgcagctg cctggtggcg cgcgactgca tcgatcgttt cgagcgcggc
     2161 gccggggacg agggcgcgga aggcggctgg aacgtggtgc tgcgcggcct cgaggagcgc
     2221 ctgccggtga gccgccggca gcagcacgtg atcaaggact tcggaaggct gtgaaggcac
     2281 tgcgagccct cgtcccggcg gcgctggcgc tcgccgccgc ggccctggga gcggcggcgc
     2341 aggacgcctg ggagacgctg ggccctggcg cgatcggtgc cggccgcgcg gagctcgaga
     2401 cgcggcttgc cctgcactgc caggccgata cgtgccggcc agcggccggg acagccgacc
     2461 gattcgccgg tgtgcccgtc gagcgcatcg atctggcgtt cgacgcggac cggctggcgc
     2521 gcgtgaccgt gcgcttcgac acgcagcact acgaacgtgt gcgcgaggcc gtgcgcgatc
     2581 ggttcggcga aggcgcggac cacagctatc tggcgcgggc ggggatggcc ggcgagttcg
     2641 ccgccggcgt gttcgtctgg tccgggcccg gcctggcgct ggtgctggag caatatgccg
     2701 gcaagatcac tcgctcgcag ctggtctacg gcacgccgcg ggcgctggcc gccctggtgc
     2761 gcgacaagag cgccacgcca cgcggcgcgc ggcgcgacct gtaggcagac cgcgccagcg
     2821 cggcgaagtc cttgttcgcg gcgggctgat cgcgcgggca aggcgatgcc gggcgatgtc
     2881 gccgccggtg cgtggtaccc tgtgacaccc tcccgtggcc gctgcgttcc cgccgtgtcc
     2941 gttcccgccg ctctgaccat cgccacccgc gagagcgcgc tcgccctgtg gcaggcccat
     3001 cacatccgcg accggctcgc cgcgctgcat ccgggggtgc gcatcgcgat cctgggcgtg
     3061 accaccgagg gcgaccggcg cctcgacgct tcgctcgcca agatcggcgg caaggggctg
     3121 ttcgtcaagg agctggaaag cgcgctggcc gatgaccgcg ccgacctcgc ggtgcactcg
     3181 atgaaggacg tgccgatgca gttgccgccg gggttcgcca tcgccgccat caccgcgcgc
     3241 gaggatccgc gcgacgcctt cgtgtcgctg cgctatcggc agctcggcga gctgccgccg
     3301 ggtgcgaagg ttggcacttc cagcctgcgg cgcgaaagcc agatccgggc gcgttacccg
     3361 cagctcgacg tgcggccgct gcgcggcaac gtggacacgc ggctgcgcaa actggaccag
     3421 ggccaatacg atgccatcgt cctcgccgcg gccgggctga agcggctcgg gttggcgggg
     3481 cgcatcaccg ccattctcgg tgcggacgag agcctgccgg cggccgggca gggcgcgctc
     3541 ggcatcgagt gccgcagcga gcgcgccgat ctgctggcgc tgctgcgccc gctcgacgat
     3601 gcggccaccg cctggtgcgt gcgcgcggaa cgcgccgtga gccgcgcgct cgcgggcagc
     3661 tgcgtggtgc cgatcggcgc ttacgccgaa ctcggtgccg gcgtgctgcg cttgcgcggg
     3721 ttcgtcgcca gcccggacgg cgcccgcatg gcacgggccg agctggccgt gccgagcgcg
     3781 gacgcggacc ccgaagcggc cggccggcgc cttgccgatc agctcgtggc ggccggcgcg
     3841 cgcgagatcc tcgccgcgct gcctcggccc tgaccgggtc atgcagccgc cgctggctgg
     3901 caaagccatc ctcatcgccc gcccgcggcg tccggcgcaa gcgctggccg agcgcatccg
     3961 cgcggccggc ggtgaggccc tggtgtttcc cgtcctggag atcgatccgg tggcgcccga
     4021 tgagcagggc agagccgccc tcgccgagct cggcgccgcc cacctggcgg tattcgtgag
     4081 cgtcagcgcc gtggagcatg gattggcgct ggtgcgcgcc gccggcggct ggccggccgg
     4141 cctcggggcg gcggcggtcg gctcggccac cgcgtcagcc ctgcgcgcgc agggcatcga
     4201 gcgcgtgctg gttcccgacc agggcgggga cagcgaagcg ctgctggcgc tgcccgagct
     4261 gctggacgtg agcggccggc gcatcgtcat ttttcgcgga atgggcgggc gcgagctgct
     4321 cgcgaccacc ctgcgcagcc gcggcgcgca ggtggcgtac gtggagtgct accgacgcgg
     4381 caagcctgcg ctggacgcgg gccccgtcat cgagcgcttg caccgcggcg cgctgcacgc
     4441 ggtggtcgcc gccagcggcg aggcgctggt gaatctggtg gagctgctcc cggcccctgc
     4501 gttgcgcgcg ctgccgctcg cggtcacgca cccgaacgtg gcgcgcgccg cgcacgatct
     4561 cggcttccgc cgcgtccagg tagcgctcgg cgaggcgcac ctgatggatg cgctgatcgc
     4621 cgcgatttgc cacgactcct gagcggtttg ccacctgtag cctgctagac tcacacctgc
     4681 gcatcaacgg cccgccacgg atgaacgaac tgccgcagga ccagcctcca gcgcccgaat
     4741 cgcctgcgcc cgaatcgccg gcatccgggc gcccggctcg gggttcgcgc tggcgggcgg
     4801 ggctcgcgcg tgtgcgccag cgcataaacc tgcgcgtcgt tgcggtcgcc gtcggcttgt
     4861 tgctgctggc cggcctgtgg ctcgatacgc gcgggcgcat caacactctg cagcaggagc
     4921 tggtgcgcga gctggccgag gccggaagcc tcggcaagga gagccgccag ctcgccgcgc
     4981 aggcgagcca gaccgggcgc gacctggagt tcaagatcgg cctgctggag agtcggctgg
     5041 cggagaccca gaaccagcgc ctggcgctcg aagcgctgta cctggagctt tctcgcagcc
     5101 gcgacgagcg cgtgctggcc gaggtggagc agatcgtcct ttcgggcagc cagcagctgc
     5161 agctcgccgg caacgtgaag gcggccctgg tcgcgctgga atcggcggac agccgcttgc
     5221 agcgcagcga cagcgcgcag ttcgcggcgc tgcgccggac catcgcgcgc gacatcgagc
     5281 ggctgaagag cgccccgttc gtggatatgg tcggcatgag tctgcgcctg gacacgctcg
     5341 cgcatcaggc cgaacagttc ggcctggcga tgtacgagcg cccggcgcag ccgccgcctc
     5401 ccacgccgcg gcccgaaggc ggaggcctgg cgcggctggc gcgcgaggct tggcaggacc
     5461 tcacgggctt gatccgcatc cagcgcctcg actcccgcga agtgccgctg gtggcgccga
     5521 gccaggagtt cttcctgcgc gagaacctgc gcatgcgcct gctgtcggcg cgcatcgcgc
     5581 tcctggcccg cgacgagacc gcgttcaaag cggacgtgcg ctcggcgcgc gattggctgc
     5641 agcgctattt cgatcacggc gacaagcagg tcgacgcggc cttgagcgcc ctgaagcgtc
     5701 tggcggagac cgacgtgagc atcgatctcc cggacatttc ggccagcctg gacgcggtgc
     5761 gccagcacaa gctggcgcgc gagcgcggcc tgcgttagag gcgggcattg aggctgctta
     5821 cctggatcat cggtctgttc gcgctggcgg tggccgtgac cgtggcggcg cgcagcagcg
     5881 ccggctacgt ggtgctgacc ctgccctcgc accgggtaga gctgtcggtc aatctcgccg
     5941 tgctgctgct gcttggcgta ttcgggctgc tctatctggt gatgcgcacc gtgatcctgg
     6001 cgaccggcat gccggcacgc gctcgcgagt accgccgcag ccaggaacgc ctgcgtgctc
     6061 gcaggatgtc cgaggacgcg ctgcgcgcct acctggaggg gcgttacggc cgcgccgatc
     6121 gcgcggcgcg gcaggccgcg gagctggacg agtggccggc gctcaacctg gcgctcgcgg
     6181 cgcgctcggc ccacgagctg cgcaactatg ccgcccgcga tgcctgcctg cagcagatgg
     6241 aaacgcgcgc accgcaggag agctacctgc gcgagaccac gcgcgccgat ctgctgctgc
     6301 aggagcgccg ctatctcgac gcgctgcaca gcctgagccg gctgcaggac aagcacaccg
     6361 gcgctctgcg cctggagctc aaggcgcacc agctcgccgg caactgggag caggtgctgg
     6421 cgctgctgcc gcagctggaa aagcgcaagg tgctggagcc gccggtgctg gagcagttcc
     6481 ggcgctatgc ggtcaccgag aacctcaagc gcaaggcgct cgacggcaag agcctgcgcg
     6541 aatactggga ccggctgccg gccgccgagc gggccgacgc gcgggtggcg gcggccgccg
     6601 cgcaaagctt catcgcgctg ggggcctgcg ccgaagggca tcgcatcatc gaggagagcc
     6661 tggcgcagac ctgggatgcg gcgctgctga tgctgtacgt cgagtgcctg ccgcgggatg
     6721 cgcgcaagca tctggagcgc gccgaggagt ggctcaagca gcacccgcgc gacccggtgc
     6781 tgctgcttgc cctcgggctg gtgtgcatgc accaggcgct ctggggcaag gcacgcagct
     6841 atctggaggc gagcctggcg atcgagctca gccacaccgg ctacgtcaag ctcggcgagc
     6901 tgctcgagcg catgggcaag cccgaggagg cgagccaggt gtaccgacgc gggctggagc
     6961 tggccctgga gcagttgaag cagtgcaccg gcggacggcg cagaccgatc ctctgagacg
     7021 ccgtggttcg cgaatcgtga ttcgcggttc tcgcagtcat gacgcaggtg ccggggtgaa
     7081 ggcgtcggag aagaactcct cctccggcag gccgcactgc gaagtgaaat ccttgtgggc
     7141 ggactcgacc acgatcggcg ccccgcaggc gtacacctgg tagccggaga ggtcggggaa
     7201 gtcctccatc accgcccggt gcacgaagcc ggtgcgacct tgccacccgt cttcgggcag
     7261 ggcgtcggac accaccggca cgaagctgaa gttgccgtgc tgcgcctgcc actggcggca
     7321 caggtccgac atgtaaaggt cgcttgggcg gcggccgccc cagtagagca ccatctgccg
     7381 cccggcgccg cggtggaacg cgtgcagcag gatgctcttg atcggcgcga agccggtgcc
     7441 gctggccacg aacagcatcg gcttgtcgct gtcctcgcgc aggaagaacg tgcccagcgg
     7501 tccttcgaac cgcaggatgt ccttctcctt catgcgggtg aagacgtaat cggtgaatgc
     7561 cccgcccggc atgttgcgca cgtgcagctc caggtgcgca tcgtcctccg gcgggttggc
     7621 catggagtag gcgcgccgct tgccgtcgcg caggatgatc tccagatact gcccggaaag
     7681 gaactgcagg cgctcgttgg cgggcagcgt caggtagagc accgccacgt ccgccgccgg
     7741 gcgctcgatg cgctgcaccc ggcacggcag cttcttgatc gggatctcgc cggtggcgct
     7801 gatgatgcgc gcctcgatga ccaggtcgga caagggcttg gcctggcaga acagggcaaa
     7861 gccgagcttc ttctcgtagt ccggcaggat gtgcgccgtg tactggccgt agtccacctg
     7921 cccctccagc agcttgccct tgcaggagcc gcaggcgccg ttgcggcagc cgtagggcag
     7981 cgtgatgccc tcgcgcatgg cggcggccag cacggtctcg ccgggctcgg cggtgaaccg
     8041 gcggttgctg ggccggatcg tgatctggaa ggccatgcac gtctccctgc gagtcggcgg
     8101 cgggcgcgcc gggctcgagc gccggcgagt tcctctagaa tgaagtatgc ggagactttt
     8161 gatcgcgtgc agatcagcac cagataacga cgtcgcatgg aaccggtcct gctggtcctg
     8221 agcaacctcc ccgaccgcga gcgcgcgctg gaactggcgc gggcactggt ggacgggggt
     8281 ttggccgcgt gcgtgaacgt cttgtccggc tgcacgtcaa tctatcggtg gcggggcgcg
     8341 gtggaaaccg ccgatgaggt gccggtgttg atcaagacgc gtcaaagcct gtacccgcgc
     8401 gtcgagcagg cgatccggcg gctgcatccc tacgaagtgc cggaagtcat cgccgtgcct
     8461 ctcgctgccg gtttacccgc gtacctggag tgggtatgcg ccgagaccga gccgacggac
     8521 gcgagatgag aacgccgccc gcgatgcgca gcgggcacgc cgcgccgcgg cgcgcgtgcg
     8581 cacgcatccg cctggcgatg ttgtgcgcgt ggctggcggc accttgtgcc ccctgggcgc
     8641 agcagccgtt ggcgcgctcc gaggccgcgg tggaatccgc ttcgacggaa ggcggggtgc
     8701 tgtcccgact gcgccagttc gccggcgcgc aagccgacgc cgaaccggag ttcctgccgc
     8761 ccgatcaggc gttcaaggtg cgggtcacgg tgcgcgatgc gcacacgctc gccgccgagt
     8821 tcgtcccggc acaaggctat tacctgtacc gggacaagat cggcttcacc ctgcccgaag
     8881 gcggcccggt ggcggtggcc ggcgtggcgc tgccccccgg cgagcgcaag gatgatccga
     8941 acttcggcct caccgaggtg tatcactcgc cggtgcgggc gcgtgtcgcg ctggcgcggg
     9001 cggacagcgc cgagcagccg atcgcgctcg aggtgcgcta ccagggctgc gccgatcagg
     9061 gactgtgcta tccgcccgag aaaaaggtct tcaatctgtt gctggccgca gccgatcccg
     9121 gcgccgcgcc ctcgccaccg gtgagcgacg tgccggcgga cgagagctcg ctggccgccg
     9181 gcatcctttc tgcgggcgac ttctgggcgg tggtggcgag ctttttcggc ttcggtctgc
     9241 tgctgtcgtt cacgccctgc gtgtttccca tgatcccgat cctgtcgggc atcatcgtcg
     9301 ggcgcggaca ccaagtgacc cgacaacacg cgctcgcgct atccggcgtg tacgtcctgg
     9361 gcatggccgt cacctacgcc ctggcgggcg tggccgcggg cctcaccggc accctgctgt
     9421 cgaacgcgct gcagaacccg tgggtgctgg gcaccttcgc tgccttgttc gtggtgctgg
     9481 cgctcgccat gttcgggttc tacgagctgc agctgcccgc cgcgctgcaa agccggctgt
     9541 cgaatgccag caaccgcctc aagggcggca ccctctgggg ggtgttcgcg atgggggtcc
     9601 tgtctgcgct gatcgtcggc ccctgcgtcg cggcgccgtt ggccggcgcg ctgctctaca
     9661 tcaaccagtc gcgcgatgcg ctgctgggcg gctccgcctt gttcgccatg gcgctcggca
     9721 tgggcgcacc gatgatcgcc gtgggggtgt cggccggcgc attgctgccc aaggcgggcg
     9781 cctggatgca gacggtgaag agcttcttcg gcgtggtgct gctcgggctg gcgatctgga
     9841 tcgtctcgcc ggtgattccg tccgtggcgc acatgctgct ctgggcagcg ctgctgatcg
     9901 tgtcggccat ctacctgcac gcgatcgatc cgttgccgca caacgcttcc ggcttccgca
     9961 agttgtggaa gggcgtaggc ttgatcgcgc tgctgctggg cgtggccttg ctggtggggg
    10021 ccttgtccgg aacccgtgac atcctgcagc ccctgtcggg attgcgggtc gcgggcgggg
    10081 gcgcgagcgc ggcttcggcc gagcaaccca ggttcatcct ggttcgctcg gtagaggagc
    10141 tggagcgtga gctggcgcag gcgggcggcc ggccggcgat gctcgacttc tacgccgact
    10201 ggtgcatctc ctgcaaggag atggaacgct ttaccttcac cgacccgcgc gtgcgcagcc
    10261 gcatggaccg catgctcctg ctcaaggcag acgtcaccgc cagcacgccg gaggacctgg
    10321 cactgctgaa gcgcttcggc ctcttcggcc cacctggcac cttgttcttc ggcccgggtg
    10381 gcgagcagct tccccaccgc gtgatcggct tccaggacgc ggagcgcttc gcggcatctc
    10441 tggacgccgt gctgcgcgcc tcatgagccg ggccgcgcag gcctggttgc tcggtgcggt
    10501 ggccgtggcg gccctcgtcg cgggcgctct cctgcggccg gcgccgcgcg ggccagcgcc
    10561 ggccccggtc ccggaggggg ccgggtcgat cttgctgcaa accgtcctcc cggacctgac
    10621 gggaacaccg cagcgcgtcg atcaatggaa gggaaaggtg ctggtcgtca acttctgggc
    10681 cacctggtgc gccccatgcc gtgatgagat ccccgccctc gtcaggctgc agcgggaaat
    10741 gggcggccag ggcctgcagg tgatcgggat cgccatcgat cagctcgaga aggtgcgccc
    10801 gtacgctgcc gagatgggga tgaactaccc gatcctgatc ggcgagctcg acgccatcga
    10861 cctggcccgc caggccggca acgagctcgg cgcattgccg ttcaccgtcg tgctcgaccg
    10921 caacggcaac gccgtgcggg cagagctcgg gcgggtgacc gaggccaaac tcgccgccct
    10981 ggtccggccc ctgctgtgat tcccttgatc ttgcgtcgat cgtggcgcct tcttgctgcg
    11041 ttcagcgccg aactgacaag aaaatagcct taacctgcgg tcgactcatg ggccctcagc
    11101 cgcctggccg agggtagcgg cccctgccta tcgcgaccac gcatccggct cataagaatg
    11161 gccgccggcc aagaggaccg gtcttggccg ttgcccgttc gagcggtcaa cttccttgaa
    11221 atgctggaca aattacgcga agttgcggca aactggcccg tatggcgacc caacagaagc
    11281 ccgggcggga ggggccgcgc cgtattctgg tgctgcatgg cccgaacctc aacctgctcg
    11341 gcacgcggga gccgtcgatc tacggcgccg ccactctgga tgagatcaat gcgcgcctga
    11401 cagcacgcgc cgaggcggac ggagccgtcc tggtgtgctt ccagagcaac gccgaagtgg
    11461 ctttgatcga ccgcgtacaa caggccgcca gcgacggcac ccagttcatc atcgtcaacc
    11521 cggcggcttt cacgcacacc agcgtggcgc tgcgcgatgc gctggcggcg gtgaagctcc
    11581 ccttcatcga agtgcacctc tccaacgtct tcgcccgcga accgttccgg caccactcct
    11641 acttcacagc gctggcggtc gggatgatct gcgggctggg cgcgcgcggc tacgagctgg
    11701 cccttgagta tgcattgggg gactatgcgc tgggggcgac gaaaaggagc tagccgatgg
    11761 acctgcgcaa gctcaagaag ctgatcgact tggtccagga atccggaatc gccgagctgg
    11821 aggtgaccga aggcgaggaa aaggtgcgca tcagccgggt cgggcagacg gcacaggcca
    11881 tcgttcccgt cgccggcgga gcggcggcga cgccgctgcc cgccgccgcg cagccggctc
    11941 cagcggcgct cgccgccgca gccgaggaga aggtcgaggg ccatgtcgtg aagtcgccga
    12001 tggtcgggac cttctatcgt tcgccctcgc ccggggccaa gaccttcgtg gaagtcggcg
    12061 acacggtcgc cccgggcgac accctgtgca tcatcgaggc gatgaagctg ttgaacgaga
    12121 tcgaggccga tcacggcggc acgatcaaag aggtctacgt ggaaaacggg caacccgtcg
    12181 agtacggaga gccgttgttt ctgattgtgt gaggcggcga tggcgaagct atccgcgcgg
    12241 gtcccgtttg ggcggaggct aatgcgcgca cagcgcgtgt tatgccggag ccaaaacggg
    12301 caacccgtcg agtacggaga gccgctgttc ctgatcgtct aagtgttcga gaagatcctg
    12361 atcgccaacc gcggcgagat cgccctgcgc atccagcgcg cctgtcgcga gctcggcgtc
    12421 aagaccgtcg cggtgcactc ggaggccgac tcggacgcca agtacgtacg cctggccgac
    12481 gagtcggtct gcatcggccc cgccggctcg ggacacagct acctcaacat tcccgccatc
    12541 atcagcgcgg ccgaggtcac cgacgccgag gcgattcacc ccggctacgg cttcctgtcg
    12601 gagaacgccg acttcgccga gcgcgtggag aagagcggct tcgttttcat cggcccgcgc
    12661 cccgagacca tccggctgat gggcgacaag gtgagcgcca agcaggcgat gatcaaggcc
    12721 agcgtgccgg tggtgccggg ctccgacggc gcgctgccgg aggcgccgga ggcgatcatc
    12781 aagctcgcgc gcggcgtggg ctacccggtg atcgtcaagg ccgcgggcgg aggcgggggg
    12841 cgcggcatgc gcgtggtgca caccgaggcc gcgctggtca atgcggtgac catgacgcgc
    12901 gccgaggcgc aggcggcgtt cggcaacccc acggtgtact tggagaagtt cctggagaac
    12961 ccgcgccacg tcgagatcca ggtgctggcg gacgagcacc gcaatgcggt ctacctgggc
    13021 gaccgcgact gctcgatgca gcgtcgccac cagaagatca tcgaggaagc gccggcgccg
    13081 ttgatcaacg cacggctcaa ggcgcgtatc ggcgagcgct gcgccgaggc ctgccgcaag
    13141 atcggctacc gcggcgcggg cactttcgag ttcctgttcg agaaggacga gttctacttc
    13201 atcgagatga acacgcgcgt gcaggtggag catccggtca cggagatgat caccggcatc
    13261 gacatcgtgc aggcccagat tcgcgtggcg gccggccaga agctgccgtt caagcagcgc
    13321 gacatccagt tccgcggcca cgccatcgaa tgccgcatca acgcggagca cccctacaag
    13381 ttcaccccct cgcccgggcg catctccgcc ttccacacgc cgggcgggcc gggcatccgc
    13441 gtggactctc acgtctacac caattacttc gtgcccccgc actacgattc gctgatcgcc
    13501 aaggtcatct cctggggcga cacgcgcgag caggcgatcg ccaagatgcg catcgcgctg
    13561 tccgagatga tcgtggaggg gatccagacc aatctgccgc tgcatcagga gttgatgctc
    13621 gactcctcct tcgcgcgcgg tggcaccagc atccactatc tggaggagaa gctggcgcag
    13681 cagagccgtc ccgactgatc gtgagctggc tgcgggcgag cttccggatc gcgcccgcgg
    13741 ccgccgaggc gctggccgac gcgctgctcg agcgcggggc gttggccgtc gagctggcgg
    13801 acgcagacga gggcaccccg gccgagcaag ccatcttcgg tgagcccggc gcggagccgc
    13861 tgccgctctg gcgtcatcaa tcgatcagcg cgctgttccc gcgcgacgcg gatcccgagg
    13921 ccgcgatccg ggcggcagtg gcggcgctcg ggctcgaggc cgagccgagc gtgaccctgc
    13981 agccagtcgc cgagcgcgac tgggtgcgcg ccagccagca acagttcgcg ccgatcgagg
    14041 tcgccccgcg gctgtggatc gtaccctcct ggcaggcagc gcccgacccg agcgcggtca
    14101 acctgatcct cgatcccggg ctcgcgttcg gaaccggcag ccatccgacc accgggcaat
    14161 gcctgcgctg gctgcacgcc aatctcgcat ccggcgcctc cgtgctcgac tacggctgcg
    14221 gcagcggcgt gctcgccatc gcggcgaagc ggctcggcgc agggcgggtg gtggcggtgg
    14281 acatcgatcc ggtggcgctc gaggtcaccc gcgcgaacgc ggccaccaac acggccgaga
    14341 tcctcgtcgc atccccggac gccgttcccc ctggccccta cgacgtggtg ctggcgaaca
    14401 tcctcgccgg ccccctcgag ctgctcgcgc cgctgctggc gagccatgcg cccgccgggg
    14461 gccggatcgt cctggccgga atcctcaccg gccaggcggc ggacgtggcc gccgcctacg
    14521 cgccctggtg cgatctcgtg caggtttcgg agaaggacgg ctgggcttgc ctggcgggcg
    14581 cacggaaagg atagacttga cgcccgcgcc cggcttcgac tctgaatgta cacgcgctgc
    14641 ccgcaatgcg acaccgtctt ccgcgtgacg ccgcagcaac tgcaggtgtc cagcggccag
    14701 gtccgttgcg ggcgctgccg ggcggtgttc gacgcctttt cctcgctgag ctcgcatccg
    14761 cccgggcggg agccgggcgt ggccgcagag cctgccgccg aatccgcgtt caggccgccg
    14821 gcggcgcacc ccgcgggcga cgaggcgctc gcggccgcct ggcgagctcg acccgtttcc
    14881 ccgatgcccg atcccttgtt ctccaccggc tcggtgctgc cagaacccag cgcgccgcgg
    14941 gcagaagctg ccgcgccccg tgcgctgcgg gaggagacgc gcccggcggt cgctgcgcag
    15001 gaaccgctca cgcttcccga ggagctgttt ccgccgggac ttaccacgcc acggcggcgc
    15061 tggctctgga tcggggtcag cgcgctgctc gtgctcggcc tcgcggcaca gatggcgtgg
    15121 ttctttcccg gccaggtcct gatgcgcgcg ccggccctgc agccggcatt gcagcaactg
    15181 tgtgcggcgc tcgggtgcca ggtgcgcctg ccgcgcgtgc ccgagcagct gttcatcgag
    15241 tcctcggatc tgcagctgct ggacgccgcg cgaccgagcc aggtgttgct caccgcaacg
    15301 atccgcaacc gcgccgggtt tgcgcaggac ttcccgctgc tcgagctgac cctcaccggg
    15361 gacgacagcc ggaccgccgc gcggcgcgtg ttccaccccg gcgagtatct ggacgccgcg
    15421 gtggagcgcg gccgcggcat cggcgcgcaa caggaggtgg ccattcacct gtatctggac
    15481 accgccgatc tgcgcgccac cggctatcgt ctgtatcttt tcttcgcctg agcgcgagcc
    15541 atcttccggc cgcatgaacc cgcccgagct gaaacgcact ccgctgttcg acgcccaccg
    15601 gcgcgcgcac gcgcgcctca ctgacttcgg cggctgggaa atgcccttgc actacggctc
    15661 gcagatcgag gagcaccatc gcgtgcggcg cgacgccggt gtgttcgacg tttcgcacat
    15721 gctggcgatc gacgtggcgg gcggcgaggc ccgcaccttc ctcggccggc tgctcgccaa
    15781 cgacgtcgcc aagctcgctg tgccggggaa agcgctgtac tcctgcatgc tgaacgagga
    15841 cggcggcgtg atcgacgacc tgatcgtgta ctggcgcggg gaggatcgct tccgtatggt
    15901 ggtcaacgcc gggaccgcgg acaaggatct ggcgtggatc gcgcggcagc gcgatcgcct
    15961 tgcgccgggc gttgtgctgc agccgcggcg cgacctcgcc gtgctggcgg tgcaggggcc
    16021 gcaggcgcgc gagcgcgtgt ggcaggtgcg gccggcgctg cgtgcggaga gcgaagcgct
    16081 cgccgcgttc caggcgcggg agcaggggcc gctgttcgtg gcccgcaccg gctacaccgg
    16141 ggaggacggg ttcgaggtga tggttccggc cacggatgcc gaggcgctgt ggaacgatct
    16201 gctcggtgcc ggcgtcgcgc cctgcgggct gggcgcgcgc gataccctgc ggctggaagc
    16261 cggcatgaac ctgtacggcc aggacatgga cgagtccacc acgccgttcg aagcgggact
    16321 gcgctggacc gtcgccatga cgggcacgcg cgattttatc gggcgggccg cgcttgcgcg
    16381 gcgtgcgccg cgctggaact tgcttggcct ggtgctgcgc gagcgcggtg tgctgcgcgg
    16441 tcatcagccg gtgcgcggtg cgctcggccc cggcgaagtc accagcggca ccttctcgcc
    16501 caccctcggg cggtccatcg cgctggcgcg gttgccggcg gcggccacac ccggggagat
    16561 ggtggaagtg caggtgcggg accggtggct gcccgctcag gttgtaaact atccgttcgt
    16621 acggcgcggc aagtcgctgc tgccggattg aggtttcctc gcggaggttt ccagttgacc
    16681 gttcccgccg atctgaagta cgccgactcc cacgaatggg ccaggctgga ggccgacggc
    16741 accgtctcgg tgggcatcac cgatcacgcc caggatcggc tcggcgacct ggtcttcgtc
    16801 gaggcaccca aggtcgggca gaccgtggcc aaaggcaagg agtgcggggt ggtggagtcg
    16861 gtcaaggccg cctccgatct gtacgcgccg gtgtccggag aggtcgtcgc ggtcaaccag
    16921 gaactcgccg gcagcccgga gaaagtcaac cagaacgcct atgccgcgtg gatgttccgc
    16981 gtccggccca gcaatccggc ggagctcaat gcgctgttgg acgccgccgc gtatgagaag
    17041 gtggttgccg ccgatcagca ctgaggagcc ggcgccgtct gactccggac ccccgattcc
    17101 agttgcctga atgcccttca ttccccacac cgacgacgac gtccgggcca tgctggcgtc
    17161 gatcggcgtg ccgcacatcg agagcctgtt cgacgagatc ccgcagacgc tgcgagcggc
    17221 gcgcctgacc gcggtgccgc cccagctggg cgagatggac gtcatgcgcc tgatgcagga
    17281 gcgcgccgag cgcgacgggc gctggctcaa cttcgtcggc gcgggcgcgt acgagcatca
    17341 catcccggct gcggtgtggc agatcgtcac gcgcggcgag ttctactccg cctatacgcc
    17401 ataccaggcc gaggccagcc agggcacgct gcaggtgctc tacgagtatc agtcgatgat
    17461 ggcgtcgctc accggcctgg acgccaccaa cgccagcctc tacgacggcg cctcggcgct
    17521 ggccgaggcg atcctgatgg cggtgcgcct gcacaagtcg tcgcgacggg tgctggtccc
    17581 ggcctcggtg catccgactt acgtcaaggt cgcgcgcacc atcgtctcca gccaagacat
    17641 cgagctgatc gagctgccgt actgcaccga gcagggcact acgctgcccg agtcgctgca
    17701 gcgcttcggg gagaaggcgt tcgcagcgct ggtgatcccg cagccgaatt tcttcggtgc
    17761 actcgagcag gtgcatgcgc tgaccgactg ggcgcatgcg caagccgcgc tggtcatcgg
    17821 tgtagtgaac ccggtcgcgc tggcgttgct cgtgccgccc ggacgctggg gcaagcgcgg
    17881 ggcggacatc gccgtgggcg agggccagcc gctgggcgtg ccgctcgcca atggcgggcc
    17941 ctacttcggc tttctggcct gcaagcaggc gcacgtgcgg cagatgccgg ggcgcgtcgc
    18001 cggccgcacc gtggacctcg acggccagcc cggcttcact ctgacgctgc aggcgcgcga
    18061 gcagcacatc cgccgctcta aggccacttc caacatctgc accaatcaag ggctggcggt
    18121 caccgccgcg accatccaca tggcgctgtt ggggccgcag gggctgcagc gcgtcgctca
    18181 agcctgccat gcgaatctgc acctgctgct cggccagctc gagcgcgaga ccggtgttcg
    18241 gcgccggttc gcagcgccgg tgttccacga ggccgtcatc gcgctcggcg cgcccgccgc
    18301 gccacttctg gagcgcatgc gcaagcagcg cgtcctgggc gggctcgctg tgggcggcga
    18361 ctatccggag ctcaccgaca gcatcctggt gtgcgccacc gagaccaaga ccccgggcga
    18421 cctcgaccgc tacgtcgaag cgctcatcgc cgcccgccgc gcgggttgat tccgaaacaa
    18481 ccagaacagg aggagcacgt ttcatggcaa gcgtcacctt gaaaggcaac ccggtgctcg
    18541 tcgccggtca actgccgcaa aagggc
//
LOCUS       DASPGE010000349        12993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_326926, whole genome shotgun sequence.
ACCESSION   DASPGE010000349 DASPGE010000000
VERSION     DASPGE010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12993
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326926"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1492)
                     /locus_tag="VD791_11225"
     CDS             complement(107..1492)
                     /locus_tag="VD791_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXV08593.1"
                     /translation="MEWIATHLSLILFFVLAIALFCGYPVAFVLGGIAILFGAIGMLL
                     DVFMPVQFFNLLPRIWGSAASNPILVAVPMFIFMGTVLERSGVANDLLHCLQVLLRRI
                     PGGLAMSVTLMGTIMAATTGIVGASVVMLTLIALPAMMQRGYDKKLAVGTIASAGTLG
                     ILIPPSIMLVIMGDLLSVSVGSLFAAAVIPGLILSLLYLIYIGSLSFYKPELAPPLPK
                     DVGPKTSGEFWRMILKSFVPPTFLIVLVLGSIFGGWATPTEAAGVGAFGALLLAWWNK
                     RLDLKTLKETIEGAALTNAMVFFIFFGATMFSFVFRSLGGDEVVVDLLAKAGLDTGWE
                     ILIFMMVLVFVFGFFFDWIEICLIVLPVFAPVLQGVDFGPHLGEGGPILFLTWFVIMM
                     SVNLQTSFLTPPFGFTLFYMKGTVPKEVTMAHIYRGIAPFVILQLVGLMLVIAFPELA
                     LWLPRAAGFLN"
     gene            complement(1503..2069)
                     /locus_tag="VD791_11230"
     CDS             complement(1503..2069)
                     /locus_tag="VD791_11230"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HXV08594.1"
                     /translation="MERMLAASDGINALLGRVGAAVGWLFLAATLVIIFDVLSRKFGF
                     QIPGMGSTRLQELEWHLHTALFSFWLGAGYIRNTHVRIDVALTKASPRTHALLELLGC
                     LVFAIPYSVIAIYFAADFTWISFVDNESSESANGLPWRWIPKSFILFGLVLLLAAVIS
                     VLMRVIVFLFGPERLRRASAYGAARMPG"
     gene            complement(2152..3207)
                     /locus_tag="VD791_11235"
     CDS             complement(2152..3207)
                     /locus_tag="VD791_11235"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXV08595.1"
                     /translation="MRRLPKVVFAGAAVLILSADLPVLAQDKKVKINVANNFPNSLPI
                     VGESGPRYAERVRRVSAGGIDMKFHEPGALVPALQSIQAVSKGSVDAAWSSPGFYSGT
                     DSAFNMFSTVPFGPAIPEYLAWMYYGGGRELQDQMFARHNIHAIPCGIHAPEASGWFR
                     KEIKSVNDLKGLKMRFFGLGAKVMERLGVSTQLLAPGDIFQALQLGTIDATEFSMPVL
                     DQKQGFHQVAKYYYFPGWHQQASIIDLFVNKAKWDALSDQHKAILEVTCGDMMREVAA
                     QSEAIQYKAMREMRDKNGVKILYWSPEIMKAYEKAWGEVVAEESAKNPNFKKVWDSYA
                     KFRAEYDIWREHGYLKN"
     gene            3435..3956
                     /locus_tag="VD791_11240"
     CDS             3435..3956
                     /locus_tag="VD791_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08596.1"
                     /translation="MLEELLAGPGARTLVHWLALPETRLAVVAPGLLSLAILGFYVRA
                     GRISHRLQLLWVLALPISYFCAHWQDSGGTERLYIYSAFSVVCALLVFMRIYLPPALA
                     FALTFLSLWWVDVTHALCRALECGIPLDQFYLGVGGGGARDALLLVPAMTAAMVAYAT
                     RRIRARGEALVEL"
     gene            complement(3967..4587)
                     /locus_tag="VD791_11245"
     CDS             complement(3967..4587)
                     /locus_tag="VD791_11245"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HXV08597.1"
                     /translation="MKHITATFLRGLVTLLPILLTAYILYWLAVTAESLLGRPLKALL
                     PDWIYRPGLGVLVALALVFGVGLVMELYVARRLWALLERAVLRVPVVKTLYGAIRDFA
                     GFVSESSKQKGMGQVVRVRVADHMHLIGFVTREDFAALPAALGEADVIAVYLPMSYQV
                     GGYTVLLPRRLVEPIDMSVEEALRFAVTAGMSQTAGRSDDGLAPLG"
     gene            complement(4620..5273)
                     /gene="larB"
                     /locus_tag="VD791_11250"
     CDS             complement(4620..5273)
                     /gene="larB"
                     /locus_tag="VD791_11250"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HXV08598.1"
                     /translation="MDHDIKLDLQRAARIGFDEAVFCSGKTVGHIGRILDQAQDKGMS
                     LLLTRLHEAQFAELPEHHRGRIDYEPVSRTGFFGAAHAPEGQPRVAVVAAGTSDVFVS
                     REAARTLRYYGVPNLEITDVGVAGLWRLLERLEEIRRMPVVIAVAGMDAALPTVLGGL
                     VPGLVIGVPTSVGYGVAANGQSALHAMLSSCTPGLAVMNIDNGYGAACAAMRALRIR"
     gene            complement(5276..6091)
                     /locus_tag="VD791_11255"
     CDS             complement(5276..6091)
                     /locus_tag="VD791_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08599.1"
                     /translation="MGEQLPELAALASVLTGMRAIAVAVSGGVDSLTLATHAHRLLPQ
                     RVTMFHAVSPAVPDEATGRTRELARREGWALHVIDAGEFGDLEYMRNPVNRCFYCKTN
                     LYGAIRPHTGAQIVSGTNLDDLGEYRPGLIAAQQHGVRHPFVEAAMTKQRVRALAREL
                     GLPEIAELPASPCLSSRVETGIAIDPAVLGAIHAAERLVSDTLRPKTVRCRVRASAVV
                     IELDQAALATLDGAQRAALSEQIVERFRPTGVRAPVRFEPYRTGSAFLHFKPS"
     gene            complement(6084..7349)
                     /locus_tag="VD791_11260"
     CDS             complement(6084..7349)
                     /locus_tag="VD791_11260"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HXV08600.1"
                     /translation="MSPLHIHLDAVGGVAGDMFVAALCAAFPEQRAGLLAALQRAGLP
                     PEVQCWFAEHADHALTGLRFLVHDPQEEAQQQPHLRERRHLHHERSFADIRAALLAST
                     LEPEVKRRAVAIFTLLAEVEGEIHGVPADRVSFHELGGNDSIADIVGAAYLIAAFPGA
                     TWSVSALPLGRGRVQTAHGMLPVPAPAAARLLQGFDFVDDGVEGERVTPTGAAILRHL
                     GAEPGAKRGGARTPRRLIGTGHGFGARTFPALSNVLRALIFEEQPVQGADRKVALIAF
                     EVDDQSPEDLAIALDHLRAHPGVLDVLQMPAFGKKGRMLTHLQILAEPAAAEDVFEAC
                     FNETTTLGLRWQTVERRVLARRQRTVQHAGRSVRVKVAERPEARTAKAESDDLLALSG
                     GRAAREQARRAAEDEGLRGGDEEGSKNHG"
     gene            complement(7436..8758)
                     /locus_tag="VD791_11265"
     CDS             complement(7436..8758)
                     /locus_tag="VD791_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08601.1"
                     /translation="MIGDRIEVNIAGGLDVALPRMVHIRQKFKTQKVANVSAVVAEQF
                     KRPEVRAKVKPGMSIAVGVGSRGIANIAECTKQVIAELKAIGARPFVFPAMGSHGGAT
                     AEGQQQVLTDYGVTESYVGCPIRSSLEVVQLGSVDGMPVYMDKLASEADGFAFVCRIK
                     PHTNFRAPIESGIVKMMTIGMGKITGATELHTNGMDAFGELLPKVARFIMSKKSFLFG
                     VGMVENAADETALIEAVPAEQLWEREPVLQQKAKDMMARICFDEVDVLIIEHMGKNIS
                     GSGFDPNITGRNARFIEWNMKPLVKKIVVLGLTPETHGNATGVGSADVITMRLYKEID
                     IAKTYTNIIASTYLDGAGIPMIMNTDEDAIKLAAKTVVRVKPQDTRIVRIRNTLELVD
                     IQVSEPLMAQVRARPEQFEVVGQPEAFKFDAKGTLYPMLAPHREAAHA"
     gene            complement(8797..10266)
                     /gene="glgA"
                     /locus_tag="VD791_11270"
     CDS             complement(8797..10266)
                     /gene="glgA"
                     /locus_tag="VD791_11270"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HXV08602.1"
                     /translation="MSSAPSKVSVLFATSEAAPLVKTGGLADVSGSLPVALTHQGVDL
                     RVLMPGYPAVMEGVKSKGRVASFPALGELPASQVLAGKLAGGVPLLIVDCPPLYDRPG
                     GPYQDTTGKDWPDNDLRFGLLCYVASLLASSHSPIGWRPQVLHCNDWQTGLAPAYLRY
                     VPGTTARTVFTIHNLAYQGIFPPSAVSRLGLPPAAFGPDGVEYYGNVSFLKAGLFYAD
                     HLTTVSPNYAREIQREPLGMGMQGLLAHRSATLTGILNGIDTDAWDPESDPYIAKYYN
                     ASRLPVKLENKRALQTRMNLVAGDEVPLIGCIGRFTPQKGFDLVLACTDDLLALPAQL
                     AVLGSGDSALQEAFQRLARRHPRRVGVQVGYDESLAHQIEAGADIFLMPSRFEPCGLN
                     QMYSQRYGTPPVVHATGGLVDSVTDTTQETIAAKTATGFLFSPMTRMALLGGVRRALG
                     AYRDRKLWRQLQKNGMARDFSWEASAQRYLELYQALVAA"
     gene            complement(10266..12473)
                     /gene="glgB"
                     /locus_tag="VD791_11275"
     CDS             complement(10266..12473)
                     /gene="glgB"
                     /locus_tag="VD791_11275"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HXV08603.1"
                     /translation="MTPPSSAPSGVPVQADPLVEALLAARLHDPFSLLGLHRVGERWR
                     LRVFNPHAEAVWLRLPSGLEPLDPVHPAGLFEWRGAEPPAMPYVLGVSAGGEVREMHD
                     PYAFVPALSGHDLYLFNEGRLRQAWSTLGSHPEERHGVQGVRFAVWAPNAERVSVIGD
                     LNRWDGRVHPMAAHGSSGVWELFIPGLRGGELYKYEIRNRASGEVLVKTDPYAQEYER
                     RPGTAARVRGAAPYRWQDAAWLERRAQWDWQHAPMSIYEVHLGSWKRHPDGRFHSYRE
                     LAGELVAYVRDMGYTHVELLPVSEHPLDESWGYQTTGYFAATTRYGSPDDLRALIDAF
                     HQAGIGVFVDWVPAHFPQDAWALARFDGTALFEHEDPRLGLHQDWGTHIFNYGRNEVR
                     SFLLSSAHYWLSEFHADGLRVDAVASMLYLDYSRLPGEWLPNKYGGRENLEAIEFLRA
                     LNVMVHEQFPGALTLAEESTAWPMVSRPAWLGGLGFSLKWNMGWMNDTLSYMRLDPIH
                     RRYHHDQLTFGQLYAYTENFLLPLSHDEVVHGKGSLLDKMPGDTWREFANLRLLFVYQ
                     YTAPGKKLNFMGNEIAQGREWQARWELDWSLLAIDWHAGVQRLTRDLNRLHRESPALH
                     DQDFAQEGFSWIDCHDSDHSTLSFLRRARDGSFVVVVFNFTPVPRQEYRIGLPTGGRY
                     EEILNSDSAYYGGSNAGNAGGVQAEALPWMGLAFSAAITLPPLAGIVLRAEPR"
     gene            12709..>12993
                     /locus_tag="VD791_11280"
     CDS             12709..>12993
                     /locus_tag="VD791_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXV08604.1"
                     /translation="MAQEATIPQQTRTSSPALDVRFVSTLTSRTYALILAGGRGSRLM
                     QLTDWRAKPAVFFGGKFRIIDFPLSNCVNSGIRRIGVATQYKSHSLIRHLQ"
BASE COUNT         2132 a         4498 c         4307 g         2056 t
ORIGIN      
        1 gcggtcaagg gcgatctgaa cctgaagtaa ggcgagcgcg ttcgatccac cgagccgggc
       61 cttcggcccc ggctcggtcc ttttcggttg tttctccacg acccgatcag ttcaagaagc
      121 ccgcggcgcg cggcagccac agcgccagct cgggaaacgc aatcaccagc atcaacccca
      181 cgagctgcag gatcacgaac ggcgcgatgc cgcgataaat gtgcgccatc gtcacctcct
      241 tcggcaccgt acccttcatg tagaacaggg tgaagccgaa cggcggggtg aggaaggagg
      301 tctgcaggtt caccgacatc atgatgacga accaggtcag gaacaggatc ggcccgccct
      361 cacccaggtg cgggccgaag tccaccccct gcagcaccgg cgcgaacacc ggcagcacga
      421 tcaggcagat ctcgatccag tcgaagaaga aaccgaacac gaacaccagc accatcatga
      481 agatcaggat ctcccagccg gtgtcgaggc cggccttggc cagcaggtcc accaccacct
      541 cgtcgccgcc gagcgagcgg aacacgaacg agaacatggt ggcgccgaag aagatgaaga
      601 acaccatggc gttggtgagc gcggcgccct cgatggtctc cttcagcgtc ttcagatcca
      661 ggcgcttgtt ccaccaggcg agcagcagcg caccgaaggc gccgacgccg gcggcctcgg
      721 tgggcgtggc ccagccgccg aagatggagc ccagcaccag cacgatgaga aaagtcggcg
      781 gcacgaagct cttcaggatc atgcgccaga actcgccgct ggtcttcggc ccgacgtcct
      841 tgggcagcgg cggcgccagc tccggcttgt agaagctcag gctgccgatg tagatcagat
      901 acagcagcga caggatcagt cccgggatca ccgccgcggc gaacagcgac cccaccgaca
      961 ccgacagcag gtcgcccatg atcaccagca tgatcgaggg cggtatcagg atccccagcg
     1021 tgcccgccga ggcgatggtg cccaccgcaa gtttcttgtc gtaaccgcgc tgcatcatcg
     1081 ccggcagcgc gatcagcgtg agcatgacca cggaggcgcc gacgatgccg gtggttgcag
     1141 ccatgatcgt gcccatcaac gtcaccgaca tggcgagccc gcccgggata cgacgcagca
     1201 gcacctgcaa gcagtgcagc aggtcgttgg ccacgccgct gcgctcgagc acggtgccca
     1261 tgaagatgaa catcggcacc gccaccagga tcgggttgga agcggccgag ccccagatgc
     1321 gcggcagcag attgaagaac tgcaccggca tgaacacgtc cagcagcatg ccgatggcgc
     1381 cgaacaggat ggcgatcccg cccagcacga aggcgaccgg gtagccgcag aacagcgcga
     1441 ttgccagcac gaagaacagg atcagcgaca ggtgcgtagc aatccattcc atggcgcggc
     1501 cctcagcccg gcatcctggc ggcgccgtac gcggacgcgc ggcgcagccg ctccggaccg
     1561 aacagaaaca cgatcacgcg catcagcacc gagatcacgg cggcgagcag cagcaccagc
     1621 ccgaacagga tgaagctctt cgggatccag cgccacggca gaccgttggc cgattccgag
     1681 ctctcgttgt ccacgaacga gatccaggtg aaatcggcgg cgaagtagat cgcgatcacg
     1741 gaatagggaa tcgcgaagac caggcagccg agtagctcga gcaaggcatg ggtgcgcggg
     1801 ctggccttgg tgagggcaac gtcgatgcgc acgtgggtgt tgcggatgta gcccgcgccc
     1861 agccagaaag agaacagcgc ggtgtgcagg tgccactcca gctcctgcag gcgggtggag
     1921 cccatgcccg ggatctggaa cccgaacttg cgcgacagca cgtcgaagat gatcaccagc
     1981 gtggcggcca ggaacagcca gcccaccgcc gcgccaaccc tgcccaacaa ggcgttgatg
     2041 ccgtcgctgg ccgcgagcat ccgctccatg tctcctcccg atccgttctt gttccgcggc
     2101 ccgtggggcc ggtgcaaccg gcaaccgtgc tgacctgccg tgcagggccg gtcagttctt
     2161 caggtatccg tgctcgcgcc agatgtcgta ctcggcgcgg aacttggcgt aggaatccca
     2221 caccttcttg aagttgggat tcttcgccga ctcctccgcc accacttcac cccaggcttt
     2281 ctcgtaggcc ttcatgatct ccggcgacca gtacaggatc ttcacgccgt tcttgtcgcg
     2341 catctcgcgc atcgccttgt actggatcgc ctcgctctgc gcggccacct cgcgcatcat
     2401 gtcgccgcag gtcacctcga ggatcgcctt gtgctggtcg gacaaggcgt cccacttcgc
     2461 cttgttgacg aacagatcga tgatcgacgc ctgttgatgc cagcccggga agtagtagta
     2521 cttcgccacc tggtggaacc cttgcttctg atcgagcacc ggcatggaga actcggtcgc
     2581 gtcgatggtg cccagttgca gcgcctgaaa gatgtcgccg ggcgcgagca gctgggtgga
     2641 tacgccgagg cgctccatca ccttcgcccc caggccgaag aagcgcatct tcaggccctt
     2701 caggtcgttg acgctcttga tctccttgcg gaaccagccc gaggcctcgg gcgcgtggat
     2761 gccgcagggg atggcgtgga tgttgtgcct ggcgaacatc tggtcctgca gctcgcgccc
     2821 gccgccgtag tacatccagg cgaggtactc cggaatcgcc gggccgaagg gcaccgtgga
     2881 gaacatgttg aaggccgagt ccgtcccgga gtagaaaccg gggctcgacc aggcggcatc
     2941 caccgatccc ttcgacaccg cctggatcga ttgcagcgcc ggaaccaggg cgcccggctc
     3001 gtggaatttc atgtcgatgc cgccggcgga cacgcgccgc acgcgctccg catagcgcgg
     3061 cccggattcc ccgacgatgg gcaggctgtt cgggaagttg ttggcgacgt tgatcttgac
     3121 cttcttgtct tgcgccagga caggcaggtc ggcgctcaag atcagcacgg ctgcgccagc
     3181 gaacacgacc ttcggcagtc gtcgcacggg tctcctccct ccgttcgttc cgatacctcg
     3241 gctactcgcg tccggtgccg ggacccaagg agccgttttg gcggcattag agacgctttg
     3301 ccgcccgatg tccagcgccg ccccgcggcg cggctgtttt cgcgcgccgc tggtcagacg
     3361 ccgcgcggcg cgttaacatc ctcgacagag acctattcca ccctagcttt taacaatcgg
     3421 gaggcccacc tcctatgctc gaagagctgc tcgccggccc cggcgcacgc acgctggtgc
     3481 attggcttgc gctgccggag acgcgactgg ccgtggtggc ccccgggctg ctgtcgctgg
     3541 cgatcctcgg cttctacgtc cgcgctggcc gcatctcgca ccggctgcag ctgctctggg
     3601 tgctggcgtt gcccatcagt tacttctgcg cgcactggca ggacagcggc ggaaccgaaa
     3661 ggctgtatat ctatagcgcc ttttcggtgg tgtgcgcgct gctcgtgttc atgcgcatct
     3721 atctcccccc cgcgctcgcc ttcgccctca ctttcctgtc gctgtggtgg gtggacgtca
     3781 cgcatgccct gtgccgcgcg ctggaatgcg gcattccgct ggatcagttc tacctgggag
     3841 tgggcggcgg gggcgcgcgc gatgcgctgc tgctggtccc cgccatgacc gcagccatgg
     3901 ttgcctacgc cacgcggcgc attcgcgcgc gcggcgaggc gctggtggag ctgtgacggc
     3961 cagtccctag ccgagcggcg cgagaccgtc gtcggaccgc cccgcggtct gcgacatccc
     4021 ggcggtgacg gcgaagcgca gcgcttcctc caccgacatg tcgatcggct ccaccagccg
     4081 tcgcggcagc agcaccgtgt agccgccgac ctggtagctc atcggcaggt agaccgcgat
     4141 cacgtccgct tcgccgagcg cagccggcag cgcggcgaag tcctcgcgcg tgacgaagcc
     4201 gatgaggtgc atgtgatcgg ccacgcgcac gcgcaccacc tgccccatcc ccttctgctt
     4261 cgaggactcg gacacgaagc cggcgaagtc ccggatcgcg ccgtacaggg tcttcaccac
     4321 cggcacgcgc agcacggcgc gctcgagcag tgcccacaac cgccgcgcca cgtacagttc
     4381 catcaccagg ccgacgccga acaccagggc cagcgccacc agcactccca ggccgggccg
     4441 gtagatccag tccggcagca gcgccttcaa gggcctgccg agcagcgact cggcggtgac
     4501 ggcgagccag tacaggatgt aggccgtgag caggatgggc agcagcgtga ccagcccgcg
     4561 cagaaacgtc gctgtgatgt gcttcaaggc cgctcccttc agggcgtggc tgacgtgcgc
     4621 tagcggatgc gcagggcccg catcgcggcg cacgcggcgc cgtagccgtt gtcgatgttc
     4681 atcacggcga gcccgggcgt gcagctggac agcatggcgt ggagcgcgga ctggccattg
     4741 gccgccacgc cgtagcccac cgatgtgggc acgccgatga cgagtcccgg caccaggccg
     4801 cccaggaccg tcggcagcgc cgcatccatg cccgccaccg cgatcaccac cggcatgcgg
     4861 cggatttcct cgaggcgctc cagcagccgc cacagcccgg ccacgccgac gtcggtgatc
     4921 tccaggttgg gtacgccgta gtagcgcagc gtacgcgccg cctcacggct gacgaacacg
     4981 tccgaggtgc ccgcggccac cacggcgacg cgcggctggc cctcgggcgc atgcgccgcg
     5041 ccgaagaagc cggtgcgcga caccggctcg tagtcgatgc gcccgcggtg atgctccggc
     5101 agctcggcga actgcgcttc gtgcaggcgc gtgagcagca gggacatgcc cttatcctgc
     5161 gcctggtcga ggatgcgccc gatgtggccg acggtcttgc ccgagcagaa caccgcttcg
     5221 tcgaagccga tgcgcgcggc gcgctgcagg tccagcttga tgtcgtgatc catcgtcagc
     5281 tgggtttgaa gtgcaaaaag gcgctgccgg tgcgataggg ctcgaagcgc accggggcgc
     5341 gcacgccggt aggacgaaag cgctcgacga tctgctcgct cagcgccgcg cgctgcgcgc
     5401 cgtccagcgt ggcgagcgct gcctggtcca gctcgatcac caccgcgctg gcgcgcacgc
     5461 ggcagcgcac cgtcttcggc cgtagcgtgt cgctcaccag ccgctcggcg gcgtggatcg
     5521 cgcccagcac ggcgggatcg atggcgatgc cggtttccac gcggctcgac aggcagggtg
     5581 aggccggcag ctcggcgatc tccggcaggc ccagctcgcg cgccagcgcg cgcacgcgct
     5641 gcttggtcat cgccgcctcg acgaacgggt ggcgcacgcc gtgctgctgc gcggcgatca
     5701 gccccgggcg gtactcgccc agatcgtcga ggttggtgcc ggacacgatc tgcgcgccgg
     5761 tatgcgggcg gatcgcgccg tacaggttgg tcttgcagta gaagcagcgg ttcaccggat
     5821 tgcgcatgta ctcgagatcg ccgaactcgc cggcgtcgat aacgtgcagc gcccagccct
     5881 cgcgccgggc gagctctcgg gtgcgcccag tggcctcgtc cggcacagcg ggcgagacgg
     5941 cgtggaacat ggtcacgcgt tgcggcagca ggcgatgggc atgggtggcc agcgtcaagc
     6001 tgtcaacgcc accgctcacc gccaccgcga tcgcgcgcat cccggtcagc acgcttgcca
     6061 gcgccgccag ctccggcagt tgctcaccca tggttcttcg atccctcctc gtcaccgccc
     6121 cgcaagccct cgtcttccgc cgcgcgacgt gcctgctccc gcgctgcgcg cccgccgctc
     6181 agcgccagca ggtcgtcgct ttcggccttc gcggtgcgcg cctccggccg ctcggcgacc
     6241 ttgacgcgca cgctgcgtcc cgcgtgctgc actgtcctct gccgccgcgc cagcacgcgc
     6301 cgctccaccg tctgccagcg cagtcccagg gtagtggtct cgttgaagca cgcctcgaac
     6361 acgtcttccg ccgccgccgg ctcggccagg atctgcaggt gcgtgagcat gcgccccttc
     6421 ttgccgaagg caggcatctg cagcacgtcc agcacacccg gatgcgcgcg caggtgatcc
     6481 agggcgatgg ccagatcttc cggcgactga tcgtccactt cgaaggcgat cagcgcaacc
     6541 ttgcgatctg cgccctgcac cggctgctcc tcgaagatca aggcgcgcag cacgttgctc
     6601 agcgccggga aggtcctggc gccgaagccg tgcccggtgc cgatcaggcg gcgcggtgtg
     6661 cgggcgccac cccgcttggc gcccggctcg gcgcccaggt gcctgagaat cgccgcaccg
     6721 gtcggggtga cgcgctcgcc ctcgacgccg tcgtcgacga agtcgaaccc ctgcagcagg
     6781 cgcgctgccg ccggcgcagg caccggcagc atgccgtgcg ccgtctgcac gcggccgcgc
     6841 ccgagcggca gcgccgacac gctccaggtc gcgccgggaa acgctgcgat caaatacgct
     6901 gcgccgacga tgtcggcgat ggagtcgttc ccgccaagct catgaaacga cacccgatcc
     6961 gccggcacgc catggatctc cccttcgacc tcggcgagca gggtgaagat ggcgaccgct
     7021 cgtcgcttga cttcgggctc gagggtgctc gcgagcaggg ccgctcgaat gtcggcgaaa
     7081 gagcgttcgt ggtgcagatg acgccgctct cgcaagtggg gctgttgctg cgcctcttct
     7141 tgcggatcgt gcaccaggaa gcgcaagccg gtcagcgcat ggtccgcgtg ttcggcgaac
     7201 cagcactgca cttcgggcgg caggccggcg cgctgcagcg cggccagcag gcccgcgcgc
     7261 tgctcgggga aggcggcgca gagcgccgcg acgaacatgt cgcccgccac gccgccgacg
     7321 gcgtcgaggt ggatatggag cggggacatc ggtggcaccg ctgctttccc ccgcctcgtc
     7381 gcctctcccc cgaggggcgc agtggcggtt caggaccggg ggaggaagag gctcctcacg
     7441 cgtgcgcggc ttctcgatgc ggcgcgagca tcgggtaaag cgtgcccttg gcgtcgaact
     7501 tgaacgcctc gggctgaccg acgacctcga actgctcggg acgcgcccgc acctgcgcca
     7561 tcagcggctc ggagacctgg atgtcgacca gctcgagggt gttgcggatg cgcacgatcc
     7621 tggtgtcctg cggcttgacc cgcaccacgg tcttggccgc cagcttgatg gcgtcctcgt
     7681 cggtgttcat gatcatcggg atacctgcgc cgtccagata agtcgaagcg atgatgttgg
     7741 tgtaggtctt ggcgatgtcg atctctttgt acaggcgcat ggtgatcacg tccgccgagc
     7801 ccacgccggt ggcgttgccg tgagtctccg gggtgagtcc cagcaccacg atcttcttca
     7861 ccagaggttt catgttccac tcgatgaagc gcgcattgcg gccggtgatg ttggggtcga
     7921 aacccgagcc gctgatgttc ttgcccatgt gctcgatgat cagcacgtcc acctcgtcga
     7981 agcagatgcg cgccatcatg tccttggcct tctgctgcag aaccggctcg cgctcccaca
     8041 gctgttcggc gggcaccgcc tcgatcagtg cggtctcgtc ggcggcgttc tccaccatgc
     8101 ccacgccgaa caggaagctc ttcttcgaca tgatgaagcg cgccaccttg ggcagcagct
     8161 caccgaaggc atccatgccg ttggtgtgca gctcggtggc gccggtgatc ttgcccatgc
     8221 cgatggtcat catcttgacg atgccgctct cgatcggggc gcggaagttg gtgtgcggct
     8281 tgatgcggca gacgaaggcg aagccgtcgg cttcggaggc gagcttgtcc atgtacaccg
     8341 gcatgccgtc cacgctgccc agctgcacca cctccaggct ggagcggatc gggcagccga
     8401 cgtagctctc ggtgacgccg tagtcggtca gcacctgctg ctggccctcg gcggtggcgc
     8461 cgccgtggct gcccatcgcc gggaacacga acggcctggc gccgatcgcc ttcagctcgg
     8521 cgatcacctg cttggtgcat tcggcgatgt tggcgatccc gcggctgccc acaccgacgg
     8581 cgatgctcat gccgggcttg accttggcgc ggacctcggg gcgcttgaac tgctcggcga
     8641 ccaccgcgct gacgttcgcg accttctggg tcttgaactt ctgccggatg tgcaccatgc
     8701 gcggaagcgc aacgtccagc ccgcccgcga tgttgacttc gatgcgatcg ccgatcatgt
     8761 cgtcctcctt gcccgcaatc ccgctatttt actctatcaa gcggccacca gcgcctggta
     8821 cagctccagg tagcgctgcg cactcgcttc ccagctgaag tcccgcgcca tgccgttctt
     8881 ctgcagctgg cgccacagct tcctgtcgcg gtaggcgccc agcgcgcgcc tgacaccgcc
     8941 gagcagggcc atgcgggtca tcggcgagaa caggaacccg gtggcggttt tggccgcgat
     9001 ggtttcctgc gtggtgtcgg tcaccgagtc caccagcccg ccggtggcgt gcaccacggg
     9061 cggagtgccg tagcgctggc tgtacatctg gttcagcccg cagggctcga agcgcgacgg
     9121 catcaggaag atgtccgcgc cggcctcgat ctgatgcgcc aggctctcat cgtagcccac
     9181 ctgcacgccg acgcggcgcg gatgccggcg cgcgagccgc tggaaggcct cctgcagcgc
     9241 cgagtcgccg gacccgagca ccgccagctg cgcgggcagg gcgagcaggt cgtcggtgca
     9301 cgccagcacc aggtcgaagc ccttctgcgg ggtgaagcga ccgatgcacc cgatcaacgg
     9361 cacctcgtcg ccggccacca ggttcatccg cgtctgcagc gcgcgcttgt tctccagctt
     9421 caccggcagg cgcgaggcgt tgtaatactt ggcgatatag ggatcggact ccgggtccca
     9481 ggcgtcggtg tcgatgccgt tgaggatgcc ggtcaaggtc gcggaccgat gcgccagcag
     9541 gccctgcatg cccatgccga gcggctcgcg ctggatctcg cgcgcgtagt tggggctgac
     9601 cgtggtgagg tggtcggcgt agaacagccc ggccttgagg aaggacacgt tgccgtagta
     9661 ctcgactccg tcggggccga aggcggcggg cggcaacccc aggcggctga cggcggacgg
     9721 cggaaagatc ccctggtagg cgaggttgtg aatggtgaat acggtgcgcg ccgtcgtccc
     9781 cgggacgtag cgcaggtagg caggcgccag gccggtctgc cagtcgttgc agtgaagcac
     9841 ctgcggtcgc cagccgatcg gcgagtggct gctcgcgagc agggaggcca catagcacag
     9901 caacccgaag cgcagatcgt tgtcgggcca atccttgccg gtggtgtcct ggtaagggcc
     9961 gcccgggcga tcgtagagcg gcgggcagtc cacgatcagc aggggcaccc cgcccgccag
    10021 cttgccggcc agcacctggc tggcgggcag ctcgcccagc gcggggaacg acgccacgcg
    10081 gcccttcgac ttcaccccct ccatgacggc gggatagccc ggcatgagca cgcgcaggtc
    10141 cacgccctgg tgcgtcaggg ccactggcag cgagccgctc acgtccgcca gccccccggt
    10201 tttcaccagc ggcgcggcct ccgaggtggc gaacaggacc gagaccttgc tcggcgcgct
    10261 cgacatcagc gcggctccgc gcgcagcacg attccagcca gaggcggcag cgtgatcgcg
    10321 gcggagaacg cgagccccat ccacggcagg gcctcggcct gcacgccgcc ggcgttgccg
    10381 gcattgctgc cgccgtagta ggcggagtcg ctgttgagga tctcctcata acgcccgccg
    10441 gtgggcagcc cgatgcggta ctcctgccgg gggaccgggg tgaagttgaa caccactacg
    10501 acgaagctgc cgtcgcgcgc ccggcgcaga aagctcaacg tcgagtggtc ggaatcgtgg
    10561 caatcgatcc agctgaagcc ttcttgggcg aagtcctgat cgtgcagggc gggcgactcg
    10621 cggtgcaggc ggttcaggtc gcgcgtgagg cgctgcaccc cggcgtgcca gtcgatggcg
    10681 agcaggctcc agtccagctc ccagcgcgcc tgccactcgc gcccctgggc gatctcgttg
    10741 cccatgaagt tgagcttctt gcccggcgcg gtgtactggt acacgaacag cagccgcagg
    10801 ttggcgaact cgcgccaggt gtcgccgggc atcttgtcca gcagcgaccc cttgccgtgc
    10861 accacctcgt cgtgcgacag cggcagcagg aagttctcgg tgtaggcgta gagctggccg
    10921 aaggtgagct ggtcgtggtg atagcgccgg tggatcgggt cgagccgcat gtagctcagc
    10981 gtgtcgttca tccagcccat gttccacttc aggctgaagc ccaggccgcc gagccaggcg
    11041 ggacgcgaca ccatcggcca ggcggtggat tcctcggcca gcgtcagggc gccgggaaac
    11101 tgctcgtgca ccatgacgtt cagcgcgcgc agaaactcga tcgcctccag gttctcgcgc
    11161 ccgccgtact tgttcggcag ccactcgccc ggcagacgcg aataatccag atagagcatc
    11221 gaggccaccg catcgacccg caatccgtcg gcgtggaact cggacagcca gtaatgcgcg
    11281 ctcgacagca ggaagctgcg cacttcgttg cggccgtagt tgaagatgtg cgtgccccag
    11341 tcctggtgca ggccgagcct ggggtcctcg tgctcgaaca gcgcggtgcc gtcgaagcgc
    11401 gccagcgccc acgcgtcctg cggaaaatgc gccggcaccc agtcgacaaa cacgccgatg
    11461 ccggcctggt ggaaggcatc gatcagcgcg cgcaggtcgt ccggcgagcc gtagcgcgtg
    11521 gtcgccgcga agtagcccgt ggtctggtac ccccaggatt cgtcgagcgg gtgttcgctc
    11581 accggcagca gctcgacgtg cgtatagccc atgtcgcgca cgtaggcgac cagctcgccg
    11641 gccagttcgc ggtagctgtg gaagcgcccg tcgggatggc gcttccagga acccaggtgc
    11701 acctcgtaga tgctcatcgg cgcgtgctgc cagtcccatt gcgcgcgccg ctccagccag
    11761 gccgcatcct gccaccggta tggcgccgcg ccgcgcaccc gcgccgccgt gcccggccgg
    11821 cgctcgtact cttgagcata gggatcggtc ttgaccagca cttcgccgct ggcgcggttg
    11881 cggatctcgt acttgtacag ctccccgccg cgcaggcccg gaatgaacag ctcccagacg
    11941 ccgctgctgc cgtgcgccgc catcggatgc acgcggccgt cccagcggtt gagatcgccg
    12001 atcacgctca cccgctcggc attgggcgcc cacaccgcga agcgcactcc ctggacgccg
    12061 tggcgctcct cgggatggct gcccagggtg ctccaggcct ggcgcaaccg cccttcattg
    12121 aacaggtaca gatcgtgccc gctcagcgcc ggcacgaagg cgtagggatc gtgcatctcg
    12181 cggacctccc cgccggcgct gacgcccagg acgtagggca tggccggcgg ctcggcgccg
    12241 cgccactcga acagcccggc gggatgcacc gggtcgagcg gttcgagacc cgagggcagg
    12301 cgcagccaca ccgcctccgc gtgcgggttg aacacgcgca agcgccagcg ctcgccgacg
    12361 cgatgcaagc cgagcaggct gaacggatcg tgcagccgtg ccgcgagcag ggcctcgacc
    12421 aggggatctg cctggaccgg aacaccggaa ggagcggagg aggggggtgt catgccagtc
    12481 ggcaacggaa cggtcgcgca ctcgccagcg ggccggcgct attgggcaga acgccgtcgc
    12541 ttgcctcgac gggcgcacat cggccgttgc attataagcg gcgcctctcg aaattcccag
    12601 ttcttccagg accttggcgc gccgatgcgc ggccacaagg atgccacccc gcgcatagtg
    12661 gcacaataaa cgacgttgcc agctccttcg cggcaggcac cagcgaccat ggcacaggaa
    12721 gcgacgatcc cccaacagac acgcaccagc agtccggcac tggacgtgcg gttcgtcagc
    12781 acgctcacca gccgcaccta cgcgctgatc ctggcgggcg gacgcggcag ccggctgatg
    12841 cagctcaccg attggcgcgc caaacccgcg gtgttcttcg gcggcaagtt ccggatcatt
    12901 gactttcccc tgtccaactg cgtcaactcg ggtattcggc gcatcggcgt ggccacgcag
    12961 tacaagtcgc acagcctgat ccgccacctg cag
//
LOCUS       DASPGE010000350        10479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_91705, whole genome shotgun sequence.
ACCESSION   DASPGE010000350 DASPGE010000000
VERSION     DASPGE010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10479
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91705"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VD791_11285"
     CDS             <1..456
                     /locus_tag="VD791_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV08605.1"
                     /translation="MPFADATFDVVIGQEAWCHVPDKPRLIAECARVVRPGGVIAFTD
                     ILRRAGLSAAESERLREEMSFATLETHAGYVGLLEGRGCTVHAQDDLSGHWARILVDR
                     LAMYRGLKEETVRKFGAAHFRRWDDTYAFFVNLFTAGKLGGGRFVARRA"
     gene            453..1121
                     /locus_tag="VD791_11290"
     CDS             453..1121
                     /locus_tag="VD791_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HXV08606.1"
                     /translation="MSDSPLPVLSPLEARVLAVLIEKQLTTPDYYPLTLNALVAGCNQ
                     KSSRHPLMNVAEREVQDALAALKRQTLVIDSYGASGRVMRYAHNAPKVLNIGQAMTAL
                     LAVLILRGPQTAAELRGNGERMYQFADLSSVEAYLEDMARRSAGALALKLPKRTGSRE
                     HRWAHLLSGPVASESVAAPLAEEAAGSELSALRAQVAQLSDEVAELRGLVARLQEVLR
                     AARG"
     gene            complement(1189..2505)
                     /locus_tag="VD791_11295"
     CDS             complement(1189..2505)
                     /locus_tag="VD791_11295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXV08607.1"
                     /translation="MAALLCAGVSAAAEPVPVQGDAIRRVVEILEQIRSRYVEPIDER
                     KMVSDAVNGVLKGLDSYSKYLDPEAFKKMQAENRGRYGGVGLELRMENGLPTVVATFD
                     DTPAQRAGLKPGDRISRVGDKSTEGLTLEQVVQRMRGEPDSQVDLAVLRGGEAEPRVF
                     TLTRTVIQWQSVRSKLIEPDYGYLKLTHFQEQTPQMMAGAIEQLWKQHDGGLSGIVLD
                     LRDNPGGLITAAVGVSAAFLPSDASVVSADGAGENSKMRRYAKREDYLRGRAEDYLAR
                     LPAEIKSVPLVVLVNRNSASASEIVAGALQDHKRATILGTQTHGKGSIQQIIPFDDGS
                     GLKITTSYYYTPAGHKVNGKGITPDVIVEAGSAVPRLSDAIPTLPEANAQLAKADAAV
                     TGKCGQPVRTAGGGGTEGEQEVDCQLEEALHLLRERKPDQGELSSR"
     gene            complement(2757..3731)
                     /locus_tag="VD791_11300"
     CDS             complement(2757..3731)
                     /locus_tag="VD791_11300"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HXV08608.1"
                     /translation="MKSSRTQQLRSQLALPVIAAPMFIVSNPELVIAQCESGVVGSFP
                     ALNARPKEQLEEWIARIESALEERRRTDPSARVAPFAVNQIIHQSNDRLEHDLDVCVR
                     HRVPIIITSLRSPDDVVRRVHAYGGMVFHDVISIRHAHKALEAGVDGLVLVCAGAGGH
                     AGTISPFALVREVRGFFDGPIALSGAITDGAGVLAAEAIGADFAYVGTRFIATHEANA
                     VQGYKQAILDSTAADIVYTPFFSGVHGNYLKRSIVAAGLDPDNLPVADKTSMSFASNR
                     APKAWKDIWGAGQGVGNIDQVLPARELVQRMRQEYLAAKARIGGGATG"
     gene            complement(3728..5392)
                     /gene="ettA"
                     /locus_tag="VD791_11305"
     CDS             complement(3728..5392)
                     /gene="ettA"
                     /locus_tag="VD791_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HXV08609.1"
                     /translation="MAQYVYTMLRVGKIVPPKRQILKDISLSFFPGAKIGVLGLNGSG
                     KSSLLKIMAGVDHDIEGEAVPMPGIKIGFLPQEPQLDPQQSVRESVEQGLGELLEAKQ
                     KLDAIYAAYAEPDADFDQLAAEQAKYEAVLAAAGADTEHQLEIAADALRLPPWDAKVA
                     NLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVEWLEQFLQKFPGTVVAVTHDR
                     YFLDNAAQWILELDRGHGIPWKGNYSSWLEQKEQRLEIEAKQEAARIKTMKQELEWVR
                     SNPKARQAKSKARIARFEDLASYEHQKRNETQEIFIPVADRLGDSVIEFKNVSKGFGE
                     RLLIDNLSFKIPPGAIVGIIGPNGAGKSTLFRMITGQEKPDSGEIAIGPTVKLAFVDQ
                     SRDQLPNDKTVWEAISGGADVIAVGKFEMPSRAYIGRFNFKGADQQKNVGKLSGGERG
                     RLHLARTLSAGANMLLLDEPSNDLDVETLRALEDALLEFAGCVLVISHDRWFLDRIAS
                     HILAFEGDSKVVFFDGNYQEYEADKRKRLGEEGARPHRIRFKPLKG"
     gene            5543..5839
                     /locus_tag="VD791_11310"
     CDS             5543..5839
                     /locus_tag="VD791_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXV08610.1"
                     /translation="MPYAIQTLDQPGSAELRASTRPSHVDYLTRNKDKLLAAGALIDD
                     DGGGGHGGILIVDTDDRAEAERFIAEDPFTKAGLFQKVTITRWRKAFFNKERLI"
     gene            5853..6452
                     /locus_tag="VD791_11315"
     CDS             5853..6452
                     /locus_tag="VD791_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HXV08611.1"
                     /translation="MRDERHTTPGRGEAPCSCGEETAGRRRVLRALSLAPLALAARPA
                     LAIAPSEVRSLSFRHTHTDERLSVTYHAGGAYLRDALAQIDAFLRDFRTGESHAIDQG
                     LLDVLFSLTRVLDGGVFEVISAYRSPATNAKLAANSSGVSRNSLHMQGRAIDVRLWGV
                     DTRLLRAAAIELRAGGVGFYPGQDFVHLDTGHPRTWGPA"
     gene            complement(6581..6829)
                     /locus_tag="VD791_11320"
     CDS             complement(6581..6829)
                     /locus_tag="VD791_11320"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HXV08612.1"
                     /translation="MYVQPHDLAHEFPEYMQRIDDLKAHDARIAHLCEEYERLNREIV
                     DIEENDKPFQDFEFEEMKKRRLKIKDDIYLILSGLKHL"
     gene            complement(6944..8302)
                     /locus_tag="VD791_11325"
     CDS             complement(6944..8302)
                     /locus_tag="VD791_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXV08613.1"
                     /translation="MSLYAKLRERDAQGKPLRVGLIGAGKFAAMYLAQVPNTPGVHLV
                     GIADLSPQNARASLQRVGWPAERYAAASLDQAAAQGGTHIGEDWRALVAHPAIDIVIE
                     ATGNPIAAVEHVLEVFRQGKHVVMVTVEADALCGPLLAQRARAAGVVYSLAYGDQPAM
                     ICDLVDWARAAGFNVVAAGRGHKWLPHFAQSTPETVWGYYGLTPEQARVGGLNPKMFN
                     SFLDGSKPAIESTAVCNATGLTPAPDGLAFPPAAIDELPGVMRPKAEGGQLHHKGQVE
                     VVSSLRRDGTPIDYDIRMGVWVVFEGDTEYIRRCFTEYGVRTDASGRYACLYKRWHLI
                     GLEVGISVASVGLRGEPTGCPTGLRADVVATAKRDLAAGEMLDGEGGYTVYGKLMPAA
                     DSLALGALPLGLAHEARLLRPVAAGRTVRREDVALDDRSVAARTRGEMEQVLGPPASR
                     AA"
     gene            complement(8314..9156)
                     /locus_tag="VD791_11330"
     CDS             complement(8314..9156)
                     /locus_tag="VD791_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXV08614.1"
                     /translation="MRLTPQQIEQFDREGYLLFPGLFSPQEMRPLIEEVPRLFAQQRP
                     ENVREKGTDAVRTNFAAHLYSYPFAKLARHPRMLEPVQQVFGEELYMHQFKINGKMAF
                     DGDVWQWHQDFGTWKADDWMPEPRAMNIAIFLDEVSEFNGPLMFIPGSHKLGVIDAAH
                     DVTTTSYPLWVINHDTIRALVERGGIVAPKGPAGSLLMFHSCLVHASSSNLSPWNRIS
                     VYLSLCAVSNHIRRFKRPEYIAHRDFTPLASLPDDCLLEDYPVPLPWKDGTPESALQP
                     QLAA"
     gene            complement(9307..9615)
                     /locus_tag="VD791_11335"
     CDS             complement(9307..9615)
                     /locus_tag="VD791_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08615.1"
                     /translation="MSAWSRIVLACVLSLAAGQSPAAIDCSRPKSGVDWLLCSNDRVA
                     LADHIMALAFRDAFYRAEDKDALVEDQERWRRTVRDACNDVPCLIRVYQDRTAELETW
                     "
     gene            complement(9673..>10479)
                     /locus_tag="VD791_11340"
     CDS             complement(9673..>10479)
                     /locus_tag="VD791_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXV08616.1"
                     /translation="FVHGFPEFWYEWKDLLADFGRDHLALAPDMRGYNLSSKPAEVEA
                     YRAKHLVEDLRLLLDHLGARRCVMVGHDWGGAVAWSFAAMHPERLERLVIINAPHPVI
                     FARELRDNPAQQQASAYMNLFRSAKAERVLAEDNYLRLRRSTLDAWAANGGAATEADR
                     AAYLAAWSQPGALTGGLNYYRASPLHPPDSGAPTEAPDLAPDAFLVRVPTLVIWGERD
                     HALLPGNLEGLERQAQDLRVIRIPEGSHWVVHEQPARIAGLIRAFIGRRD"
BASE COUNT         1515 a         3632 c         3634 g         1698 t
ORIGIN      
        1 atgccgttcg ccgacgccac cttcgacgtc gtgatcggcc aggaggcgtg gtgtcacgtc
       61 cccgacaagc cacggctcat cgcggagtgt gcgcgcgtgg tcaggcccgg cggcgtcatc
      121 gccttcaccg acatcctgcg ccgcgccggt ctgagcgcgg cggaaagcga acgcctgcgc
      181 gaggagatga gcttcgcgac cctggagacg cacgccggct acgtcggcct gctggaagga
      241 cgcggctgca ccgtgcacgc gcaggacgac ttgagcgggc actgggcgcg cattctcgtc
      301 gaccggctgg cgatgtaccg cgggctcaaa gaggagacgg tgcgcaagtt cggcgcggcg
      361 cacttccggc gctgggacga tacctacgcc ttcttcgtga acctgttcac cgcgggcaag
      421 ctcggcggcg ggcgattcgt ggcgcggcga gcatgagcga ttccccgtta cccgtcctct
      481 cgccgctgga ggcgcgcgtc ctcgcagtgt tgatcgagaa gcagctcacc acgcccgact
      541 actacccgct caccctcaat gcgctggtgg cgggctgcaa tcagaagagc agccgccacc
      601 cgctgatgaa cgtcgcggag cgcgaggtcc aggatgccct ggctgcgctc aagcgccaga
      661 cgctggtgat cgacagctac ggcgcttccg ggcgcgtgat gcgctacgcg cacaacgcgc
      721 cgaaggtgct caacatcggt caggcgatga cggcgctgct ggcggtgctg atcctgcgcg
      781 gcccgcagac ggccgcggag ctgcgcggca acggcgagcg catgtaccag ttcgccgacc
      841 tgtcctcggt cgaggcttac ctcgaggaca tggcgcgtcg ctcggccggc gccttggcgc
      901 tcaagctgcc aaagcggacc ggctcgcgcg agcaccgctg ggcgcatctg ctgagcggac
      961 cggtcgcgag cgagagcgtc gccgcgccgc ttgcggagga ggccgcgggc agcgagctgt
     1021 cggcgctgcg ggcccaggtc gcgcagttga gcgacgaggt ggcggagctg cgtgggctgg
     1081 tcgcgcgact gcaggaggtg ctgcgcgccg cacgcggctg aggccggcga gcagtctttg
     1141 gacttccccg gattgcgctg cgctgtatcc gggtcgcgta atgccgagct atctcgacga
     1201 cagctcgccc tggtccggct tgcgctcgcg cagcaggtgc agtgcctcct cgagctggca
     1261 gtccacttcc tgctcgcctt cggtgccgcc gccgccggcg gtgcgcaccg gctgtccgca
     1321 cttcccggtg accgcggcgt cggccttggc gagctgcgcg ttggcctcgg gcagcgtcgg
     1381 gatcgcatcg ctgagcctgg gcacggcgct gcccgcctcg acgatcacat cgggggtgat
     1441 gcccttgccg ttcaccttgt ggccggccgg cgtgtagtag tacgaggtgg tgatcttcag
     1501 cccgctgccg tcgtcgaacg ggatgatctg ctggatcgac cccttgccgt gggtctgggt
     1561 gccgaggatg gtggcgcgct tgtggtcctg cagcgcgccg gccacgatct cggaggccga
     1621 ggccgagttg cggttcacca gcaccaccag gggcacgctc ttgatctcgg cgggcagccg
     1681 cgcgaggtaa tcctcggcgc ggccgcgcag gtagtcttcg cgcttggcgt agcgccgcat
     1741 cttggaattc tctccggcgc cgtcggcgga caccaccgac gcatcgctgg gcaggaacgc
     1801 cgccgacacg cccaccgcgg cggtgatcag gcccccgggg ttgtcgcgca ggtcgagcac
     1861 gatgccggac agcccaccgt cgtgttgctt ccacagctgc tcgatggcgc cggccatcat
     1921 ctgcggtgtc tgctcctgga agtgggtgag cttgaggtag ccgtagtcgg gctcgatcag
     1981 cttcgagcgc acgctctgcc actggatcac ggtgcgcgtg agcgtgaaca cgcgcggctc
     2041 ggcttcgccc ccgcgcagca ccgccaggtc gacctgcgaa tcgggctcgc cgcgcatgcg
     2101 ctggaccacc tgttccaggg tgagcccctc ggtgctcttg tcgccgacgc ggctgatgcg
     2161 atcgccgggt ttcaggccgg cgcgctgcgc cggcgtgtcg tcgaaggtgg cgaccaccgt
     2221 cggcaagcca ttctccatgc gcagctccag ccccaccccg ccgtagcggc cgcggttctc
     2281 ggcctgcatc ttcttgaacg cttccggatc gaggtacttg gagtaggaat ccagcccctt
     2341 cagcaccccg ttcaccgcgt ccgacaccat cttgcgctcg tcgatcggct cgacgtagcg
     2401 cgagcggatc tgttccagga tctcgaccac gcggcggatg gcgtccccct gcaccggcac
     2461 cggctcggcg gcggccgaga ccccggcaca gagcaacgct gccaatcccg ctgcccagac
     2521 acgccaccgc aaggccgtgc tgcggcccgc gtcggccagg cgcacatgcc cctgcacgtc
     2581 catcccgctg tcgccggacg tcagggggtg attcttccgg ggaaaaggct tcactggaga
     2641 cctcgctgtt ttcgctcgct gttcccgtgc acagcgcgct cagggtagcg cacagccgct
     2701 gtcgctgcaa aaattgtgca cattctgcgt cgatccgggc cgcaacggaa gcggcctcac
     2761 ccggtggccc cgcccccgat ccgggccttg gccgcgaggt attcctgccg catccgctgc
     2821 accagctcgc gcgccggcag cacctggtcg atgttgccca cgccctgccc cgcgccccag
     2881 atgtccttcc aggctttcgg ggcacggttc gaggcgaagc tcatgctggt cttgtcggcc
     2941 acgggcaggt tgtccgggtc gaggccggcg gcgacgatgc tgcgcttgag atagttgccg
     3001 tgcacgccgc tgaagaaggg cgtgtacacg atgtcggccg cggtgctgtc caggatggcc
     3061 tgcttgtagc cctgcaccgc attcgcctcg tgcgtggcga tgaagcgcgt gccgacgtag
     3121 gcgaagtcgg cgccgatcgc ctccgcggcc agcacgccgg cgccgtcggt gatggcgccg
     3181 gacagcgcga tcggcccgtc gaagaatccg cgcacttcgc gcaccagggc gaacgggctg
     3241 atcgtgcccg cgtgcccgcc cgcgcccgcg cacaccagca ccaacccgtc cacgcccgcc
     3301 tccagcgcct tgtgcgcatg gcggatgctg atgacgtcgt ggaacaccat gccgccgtat
     3361 gcgtgcacgc ggcgcaccac gtcgtccggc gagcgcaggc tggtgatgat gatcggcacg
     3421 cggtggcgca cgcacacgtc caggtcgtgc tcgagccggt cgttggactg gtggatgatc
     3481 tggttcacgg cgaacggcgc cacccgggcg ctcgggtcgg tgcgccgccg ctcctcgagc
     3541 gcgctctcga tgcgcgcgat ccactcctcg agctgctcct tggggcgcgc gttgagcgcc
     3601 gggaacgagc ccaccacgcc gctctcacac tgggcgatca ccagctccgg gttggagacg
     3661 atgaacatgg gcgcggcgat caccggcagc gccagctggc tcctgagctg ttgggttcgc
     3721 gaggacttca tcccttgagc ggcttgaagc ggatgcggtg cggccgcgcg ccctcctcgc
     3781 ccaggcgctt gcgcttgtcg gcctcgtact cctggtagtt gccgtcgaag aacaccacct
     3841 tggagtcgcc ctcgaaggcc aggatgtggc tggcgatgcg gtccaggaac cagcggtcgt
     3901 gcgagatcac cagcacgcag ccggcgaact cgagcagcgc gtcctcgagc gcgcgcaacg
     3961 tctccacgtc gaggtcgttg gacggctcgt cgagcagcag catgttggcg cctgcgctca
     4021 gcgtccgcgc caggtgcagg cggccgcgct cgccgcccga cagcttgccg acgttcttct
     4081 gctggtcggc gcccttgaag ttgaagcgtc cgatgtaggc gcgcgagggc atctcgaact
     4141 tgcccacggc gatcacgtcg gcgccgcccg agatcgcctc ccagaccgtc ttgtcgttcg
     4201 gcagctggtc gcgcgactga tcgacgaacg cgagcttcac cgtcgggccg atggcgatct
     4261 cgccgctgtc cggcttctcc tgaccggtga tcatgcggaa cagcgtggac ttgccggcgc
     4321 cgttggggcc gatgatgccc acgatcgccc cgggcgggat cttgaagctc agattgtcga
     4381 tcagcagccg ctcgccgaag cccttcgaca cgttcttgaa ctcgatcacg ctgtcgccca
     4441 gccggtcggc caccgggatg aagatctcct gggtctcgtt gcgcttctgg tgctcgtagg
     4501 aggcgaggtc ctcgaagcgc gcgatgcgcg ccttgctctt ggcctgccgc gccttggggt
     4561 tggagcgcac ccactccaat tcctgcttca tggtcttgat gcgcgcggct tcctgcttgg
     4621 cctcgatctc caggcgctgc tccttctgct ccagccacga gctgtagttg cccttccacg
     4681 ggatgccgtg gccgcggtcg agctcgagga tccactgcgc ggcgttgtcc aggaagtagc
     4741 ggtcgtgggt caccgcgacc acggtgcccg ggaacttctg caggaactgc tcgagccatt
     4801 ccacactctc ggcgtcgagg tggttggtcg gctcgtcgag cagcagcatg tcgggcttgg
     4861 acagcagcag ccggcacagc gccacgcggc gcttctcgcc gccggaaaga ttcgccacct
     4921 tggcgtccca cggcggcagg cgcagcgcgt cggcggcgat ctcgagctgg tgctcggtat
     4981 ccgcgccggc ggcggcgagg acggcctcgt acttggcctg ctcggccgcg agctggtcga
     5041 agtcggcgtc gggctcggcg tacgcggcgt agatcgcgtc gagcttttgt ttcgcctcca
     5101 gcagctcgcc caggccctgc tccaccgact cgcgcacact ctgctgcggg tcgagctgcg
     5161 gctcctgcgg caggaagccg atcttgatac ccggcatcgg caccgcctcg ccctcgatgt
     5221 cgtggtccac gccggccatg atcttgagca ggctggactt gcccgaaccg ttgagcccca
     5281 gcacgccgat cttggccccg gggaagaagg acagcgagat gtccttgagg atctgacgct
     5341 tgggcgggac gatcttgccc acccgcagca tggtgtagac gtattgggcc atggaaggaa
     5401 ccgctcggga tccgcaaagc gcgcaattat gcgcgaagcg cggccccgga gcacggccct
     5461 ggcgggtggc gctggagcgc agccctaaag cacgcagcgg cggcgccgtt tatcattttg
     5521 ggttcagcaa ggaggaggac ccatgccgta cgccatccag accctggacc agcctgggag
     5581 cgccgagctg cgcgccagca cccgccccag ccatgtcgac tacctgaccc gaaacaagga
     5641 caagctgctc gcagcggggg cgctgatcga cgacgacggc ggcggcgggc acggcggcat
     5701 cctgatcgtg gacaccgacg accgcgcgga ggccgagcgc ttcatcgccg aggatccgtt
     5761 caccaaggcc ggattgtttc agaaggtcac catcacgcgc tggcgcaagg cgttcttcaa
     5821 caaagagcgg ctgatctaga gagctttctg cagtgcgtga cgagaggcac accacccccg
     5881 gaaggggaga agcaccgtgc tcttgcggcg aggaaaccgc gggccggcgc cgcgtgctgc
     5941 gggcgctgtc gctggcgccg ctggcgctcg cggcgcgtcc cgcgctggcc atcgcgccct
     6001 ccgaagtgcg ctcgctcagc ttccgccaca cgcacaccga cgagcggctc agcgtcacct
     6061 accatgcggg cggcgcttac ctgcgcgatg cgctcgcgca gatcgacgct ttcctgaggg
     6121 acttccgcac cggcgagtcg cacgcgatcg accagggcct gctcgacgtg ctgttcagcc
     6181 tgacgcgcgt tctggatggc ggcgtgttcg aagtgatatc cgcctaccgc tcgccggcga
     6241 ccaacgccaa gctggccgcc aactcatcgg gcgtgtcgcg caacagcctg cacatgcaag
     6301 ggcgcgcgat cgacgtgcga ctgtgggggg tggacacgcg cctgctgcgt gccgccgcca
     6361 tcgagctgcg cgccggcggc gtgggcttct acccgggaca ggatttcgtg cacctggata
     6421 ccggccaccc gcgcacctgg ggcccggcgt agcgctcccc ggattccgct tcgctgcaac
     6481 cgggttacgc aggaccacgc cgttcacgga tgcacagcac accccctctg cccttgcggg
     6541 agagggccgg ggtgaggggt ggatgcgaag gggaccccga ctataagtgc ttcagtccgc
     6601 tcaggatgag gtagatgtcg tccttgatct tgagccgccg cttcttcatc tcttcgaact
     6661 cgaagtcctg aaagggcttg tcgttttctt cgatgtccac gatttcccgg ttgaggcgct
     6721 cgtattcctc gcacaggtgg gcgatgcgcg cgtcgtgtgc cttgaggtcg tcgatgcgct
     6781 gcatgtactc gggaaactcg tgggcgagat cgtgcggctg gacgtacatg tttcctccga
     6841 gtggttgaag ggaacagccg gttcaaggat acgcctgggt gcgccgcaaa aacaaccccc
     6901 gccgtcgccc cccaggcaac aggcgcgccc cggggccggc ccctcaggcc gcgcgcgagg
     6961 cgggagggcc caggacctgc tccatctcgc cccgggtgcg cgccgcgacg ctgcgatcgt
     7021 cgagggcgac gtcctcccgg cgcaccgtgc gccccgcggc caccggccgc agcaaccggg
     7081 cctcgtgcgc gaggcccagc ggcagggcgc ccagcgcgag cgagtccgcg gccggcatga
     7141 gcttgccgta caccgtgtag ccgccctcgc cgtcgagcat ctccccggcg gcgaggtcgc
     7201 gcttggcggt ggccacgacg tcggcgcgca gcccggtcgg gcagccggtg ggctcgccgc
     7261 gcaggcccac gctcgccacc gagatgccca cctccaggcc gatcaggtgc cagcgcttgt
     7321 acaggcaggc gtagcggccg ctggcgtcgg tgcgcacgcc gtactcggtg aagcagcggc
     7381 ggatgtactc ggtgtccccc tcgaacacga cccacacccc catacggatg tcgtagtcga
     7441 tcggcgtgcc gtcgcgccgc agcgaggaca ccacctccac ctggcccttg tggtgcagct
     7501 gcccgccctc ggccttgggc cgcatcacgc cgggcagctc gtcgatggcg gcgggcggaa
     7561 acgccagccc gtcgggcgcg ggcgtgagcc cggtcgcgtt gcacaccgca gtgctctcga
     7621 tcgccggctt ggagccgtcg aggaaggagt tgaacatctt cgggttaagg ccgccgacgc
     7681 gggcctgctc gggggtcagg ccgtagtagc cccacaccgt ctcgggcgtg gactgggcga
     7741 aatgcggcaa ccacttgtgc ccgcggcccg cggcgacgac gttgaagccg gcggcgcgcg
     7801 cccagtccac caggtcgcag atcatcgccg gctgatcgcc gtaggccagg ctgtacacca
     7861 cgcctgcggc gcgcgcccgc tgcgccagca gcggcccgca cagcgcatcg gcttccaccg
     7921 tcaccatgac cacatgcttg ccctggcgga agacctccag cacgtgctcc accgcggcga
     7981 tgggattgcc ggtggcctcg atcacgatgt cgatcgcggg gtgcgcgacc agcgcgcgcc
     8041 agtcctcacc gatgtgcgta ccgccttgcg cggcggcctg atcgagcgac gccgcggcat
     8101 agcgctcggc cggccagccg acgcgctgca ggctggcgcg cgcattctgc ggcgacaggt
     8161 cggcgatgcc gaccaggtgc acgccgggcg tgttgggtac ctgggccagg tacatggcgg
     8221 cgaacttgcc cgcgccgatc agcccgacgc gcagcggctt gccttgcgca tcccgctcgc
     8281 gcagcttggc gtacaggctc atgctggcct ccttcaggcc gcgagctgcg gctggagcgc
     8341 ggactcgggc gtgccgtcct tccagggcag cggcaccgga tagtcctcga gcaggcagtc
     8401 gtcgggcagg ctcgcgagcg gcgtgaagtc gcggtgcgcg atgtactccg ggcgcttgaa
     8461 gcggcggatg tggttcgaca cggcgcacag gctcaagtac acgctgatgc ggttccaggg
     8521 cgacaggttg gacgacgagg cgtgcaccag gcaggaatgg aacatcagca gcgagcccgc
     8581 cggccccttg ggcgccacga tcccgccgcg ctccaccagc gcgcggatgg tgtcgtggtt
     8641 gatcacccac agcggatagc tggtggtggt gacgtcgtgc gcggcgtcga tcacgccgag
     8701 cttgtggctg cccggaatga acatcaacgg gccgttgaac tcgctgacct cgtccaggaa
     8761 gatcgcgatg ttcatggcgc gcggctcggg catccagtcg tcggccttcc aggtgccgaa
     8821 atcctggtgc cattgccaca cgtcgccgtc gaaggccatc ttgccgttga tcttgaattg
     8881 gtgcatgtac agctcttcgc cgaacacctg ctgcaccggc tccagcatgc gcgggtggcg
     8941 ggcgagcttg gcgaagggat agctgtacag gtgcgcggcg aagttggtgc gcaccgcgtc
     9001 ggtgcctttc tcgcgcacgt tctcgggccg ctgctgggcg aacaggcgcg ggacctcctc
     9061 gatcagcggc cgcatctcct gcggcgagaa cagcccggga aacagcagat agccttcgcg
     9121 atcgaactgc tcgatctgct gcggggtgag cctcatgcgc gtctcctgct cgtcggtccc
     9181 tccccgtcga gcggggcggc ggccttgcac agtgtggagc aggaatgata cgcggccggc
     9241 gcgccggtgt cccggcgcgg cggctgcggc aggcacggcc ccgccgatgg gcgggaggcg
     9301 ccccggctac caggtctcca gctcggcggt gcggtcctgg tagacgcgta tcaggcaggg
     9361 cacgtcgttg caggcgtcgc ggacggtgcg gcgccagcgc tcctggtcct cgaccagggc
     9421 gtccttgtcc tcggcgcggt agaaggcgtc gcggaaggcg agggccatga tgtggtcggc
     9481 cagcgccacc ctgtcgttgg aacacagcag ccagtccacg cccgacttgg gccggctgca
     9541 gtcgatcgcc gccggcgact ggccggcggc aaggctcagg acgcatgcga gaacgattct
     9601 ggaccaggcg ctcatgggcg actcccggaa cggatgtgtt ccggacgata acggcatgtt
     9661 ccgcagcaaa cttcagtccc ggcggccgat gaacgcgcgg atcaggccgg cgatccgggc
     9721 cggctgctcg tgcaccaccc agtgcgaccc ctcgggaatg cggatcacgc gcaggtcctg
     9781 cgcctggcgc tcgagcccct ccaggttgcc gggcagcagg gcgtggtcgc gctcgcccca
     9841 gatcaccagc gtgggtacgc gcaccaggaa ggcgtcgggc gcgaggtccg gcgcctctgt
     9901 gggcgcaccg gaatcgggcg gatgcagcgg agatgctcgg taatagttca gccccccggt
     9961 cagcgccccc ggctgcgacc aggcggcgag gtaggcggcg cggtccgcct cggtggcggc
    10021 gccgccgttg gccgcccagg cgtccagcgt cgagcggcgc aggcgcaggt agttgtcctc
    10081 cgccagcacg cgctcggcct tggcgctgcg gaacaggttc atgtacgcgc tcgcctgctg
    10141 ctgcgcggga ttgtcgcgca gctcccgcgc gaagatcacc gggtgcgggg cgttgatgat
    10201 gaccagccgc tccagccgct cgggatgcat cgcggcgaag ctccacgcca ccgcaccgcc
    10261 ccagtcgtgc cccaccatca cgcagcggcg cgcgcccagg tgatcgagca gcagccgcag
    10321 gtcctcgacc aggtgcttcg cgcgataggc ctcgacctcg gcgggcttgc tggagaggtt
    10381 gtagccgcgc atgtccgggg ccagcgccag gtgatcgcgc ccgaagtccg cgagcaggtc
    10441 cttccactcg taccagaact ccggaaaccc gtgcacgaa
//
LOCUS       DASPGE010000351         3465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1144862, whole genome shotgun sequence.
ACCESSION   DASPGE010000351 DASPGE010000000
VERSION     DASPGE010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144862"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VD791_11345"
     CDS             complement(<1..273)
                     /locus_tag="VD791_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HXV08617.1"
                     /translation="MAAKEVRFHDSARHRIVAGVNILADAVKITLGPKGRNVVLEKSW
                     GAPTVTKDGVSVAKEIELKDKFENMGAQMVKEVASKTSDVAGDGTTT"
     gene            complement(286..573)
                     /gene="groES"
                     /locus_tag="VD791_11350"
     CDS             complement(286..573)
                     /gene="groES"
                     /locus_tag="VD791_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HXV08618.1"
                     /translation="MKIRPLHDRIIVKRLEEERKTASGIVIPDTAAEKPDQGEVVAVG
                     KGKILDDGKLRPVQVKVGDKILFGKYSGQTVKVEGEELLVMREEDVMGVIE"
     gene            799..2046
                     /locus_tag="VD791_11355"
     CDS             799..2046
                     /locus_tag="VD791_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08619.1"
                     /translation="MVAFTAAQIPDIAGRRYPAGLAGPLYPAGIPIRAEAELEALIEG
                     EGIDEVVFAYSDLSHEQVMHLASRVLAKGASFRLSGPAATMLRSNRPVISICAVRTGC
                     GKSPAARWIAGLLMQAGLRVGVVRHPMPYGDLERQAVQRLATLEDLAQAACTIEEREE
                     YEPHLAAGHVVYAGVDYQRILRQVEGEADVIVWDGGNNDLPFFAPDLEIVLVDPHRLG
                     HERRYFPGEVNLRRAHVVVVAKVDTARFEDVAALRASVAQVNPRAEILEAALPARVDH
                     PERIAGRRVLVIEDGPTLTHGGMSYGAGVLAAQRLGAAQLVDPRPHAAGSIRDTFVAY
                     PHIGALLPAMGYSAEQVGALADTIAACDSDAVLVATPVDLRRLIAIDRPVARLTYAFA
                     PTQPEAFERVLSTVVARASGRPD"
     gene            2148..3152
                     /locus_tag="VD791_11360"
     CDS             2148..3152
                     /locus_tag="VD791_11360"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXV08620.1"
                     /translation="MPERSHPQLALFDEPADRPAAHPVLPAAADPDLQDLAARLPAGI
                     RLGTSSWHFPGWATLVYDRLADERMLAREGLAAYARHPLLRCVGLDRTFYTLIPASEF
                     RHYAEQVPGDFRFVVKAPMLCTATGMRGEPGAHAVANPRFLDAAFAAERFVGPCLEGL
                     GEKAGAIVFQFPPLGRVFTARPQAFAERLQAFLSALPRGPLYAVELRDHVLLTPAYRD
                     ALAASGAHHCLGLHPRMPVASEQARETALDHSGPLIVRWNLHAGFGYEEARARYAPFA
                     RLVDEDIPNRVSLARLCLDAAARREPVFVIANNKAEGSAPLTLIRLAERIVAHLPKRH
                     "
     gene            3236..>3465
                     /locus_tag="VD791_11365"
     CDS             3236..>3465
                     /locus_tag="VD791_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HXV08621.1"
                     /translation="MEDQLKAAALEYHTQPTPGKISVVPTKGLTNQRDLALAYSPGVA
                     YACKAIEADPAQAARLTSRGNLVGVITNGTAVL"
BASE COUNT          495 a         1213 c         1179 g          578 t
ORIGIN      
        1 ggtggtggtg ccgtcgccgg ccacgtccga ggtcttggac gcgacttcct tgaccatctg
       61 cgcgcccatg ttctcgaact tgtccttcag ctcgatctcc ttggccaccg acacgccgtc
      121 cttggtgacg gtgggcgcgc cccaggactt ctccagcacc acgttgcggc ccttggggcc
      181 gagcgtgatc ttcaccgcgt ccgccagaat gttcacaccc gcgacgatgc ggtggcgggc
      241 cgaatcgtga aaacgtactt ccttcgctgc catttgctct ctcctttact cgatgacacc
      301 catgacatcc tcctcgcgca tcacgaggag ctcctcgcct tcgaccttca cggtctggcc
      361 ggaatacttt ccgaacagga tcttgtcccc gaccttgact tgcaccgggc ggagcttgcc
      421 gtcgtcgagg atcttgccct tgcccaccgc gacgacctcg ccctggtcgg gcttctcggc
      481 ggcggtgtcg ggaatgacga tgccggaagc ggtcttacgc tcctcttcca ggcgtttgac
      541 gataatgcga tcgtgcaacg gacgaatctt catcagtcac ctcatggttc agtaggctga
      601 ttgctgtttc tggggtttgc gctagcggtg gacgctaacg acgggggaga tatgggcaca
      661 ggcacagccc tttcaagggg gcgcacggaa aaatccgccg cctccgggct gcgccgccgg
      721 cgcctcctga tcctgggcgc tgccggtcgg gatttccatg atttcaatac gattttccgc
      781 gatgacccga gtgccgaggt ggtcgctttc accgccgccc agatcccgga catcgccggc
      841 cggcgctatc cggccggcct cgccggcccg ctctatccgg ccggcatccc gatccgcgcc
      901 gaggccgagc tggaggcgct gatcgaaggc gaggggatcg acgaggtggt gttcgcctac
      961 agcgatctgt cgcatgagca ggtgatgcat ctggcgtcgc gggtgctggc caagggcgcg
     1021 agtttccggc tctcggggcc ggcggccacg atgctgcggt ccaaccgccc ggtgatctcg
     1081 atctgcgcgg tgcgcaccgg gtgcggcaag agcccggccg ctcgctggat cgccggtctg
     1141 ctgatgcagg cgggcctgcg cgtcggtgtg gtgcgccacc cgatgcccta cggcgacctc
     1201 gagcgccagg cggtgcagcg cctggccacg ctggaggacc tggcgcaggc ggcctgcacc
     1261 atcgaggagc gggaggagta cgagccgcac ctggcggcgg gacacgtggt ctacgccggg
     1321 gtcgactacc agcgcatcct gcgccaggtc gaaggcgaag ccgacgtgat cgtgtgggac
     1381 ggcggcaaca acgacctgcc gttcttcgct cccgatttgg agatcgtgct ggtcgatccg
     1441 caccgtctcg gccacgaacg ccgttatttc ccgggcgagg tgaatctgcg ccgcgcgcac
     1501 gtcgtggtgg tggcgaaggt cgacacggcc cgcttcgagg acgtcgctgc gctgcgcgcg
     1561 agcgtggcgc aggtgaaccc gcgcgccgag atcctggagg cggctctgcc agcacgcgtg
     1621 gatcatccgg agcgcatcgc cggccggcgc gtgctggtga tcgaggacgg gccgacgctg
     1681 acccacggcg gcatgagcta cggcgccggc gtgctggccg cgcagcggct cggcgccgcg
     1741 caactcgtgg acccgcggcc gcacgcagcg ggcagcatcc gcgacacctt cgtggcgtac
     1801 ccgcacatcg gtgcgctgct gccggcgatg ggctacagtg ccgagcaagt gggcgcgctc
     1861 gcggacacga tcgccgcttg cgatagcgac gcggtgctgg tagccacacc ggtcgatctg
     1921 cgccggctga tcgccatcga ccggccggtg gcgcggctca cctatgcatt cgcacctact
     1981 cagcccgaag cgttcgagcg cgtgttgtcc accgtcgtcg cacgcgcaag cgggcgaccc
     2041 gattgagtcg cgagtggacg atattacccc tcaccccagc cctctcccgc aaggcgagag
     2101 ggggcgtgct ctgcatgccg tcgccacggt ccggtaggcc gccgaccatg cccgagcgct
     2161 cccacccgca gctcgcgttg ttcgacgagc cggccgacag gcccgccgcg cacccggtgt
     2221 tgccggcggc ggcagatccg gatctgcagg acctcgctgc ccggcttcct gccggcatcc
     2281 ggctgggtac ctcttcctgg cacttccccg gctgggccac gctggtgtac gaccgcctcg
     2341 cggacgagcg catgctggcc cgcgaagggc tcgctgcgta cgcacggcac ccgctgctgc
     2401 gctgcgtggg cctcgaccgg accttctaca cgctgatccc ggcgtccgag ttccggcatt
     2461 acgccgagca ggtgccgggg gatttccgct tcgtcgtcaa ggcgccgatg ctgtgcaccg
     2521 ccaccgggat gcgtggcgag cccggcgcgc atgcggtggc caacccccgg tttctcgacg
     2581 cggcgttcgc cgccgagcgc ttcgtcggcc cctgcctgga ggggctgggc gagaaggcgg
     2641 gcgcgatcgt gttccagttc ccgccactgg gccgcgtgtt cacggcgcgc ccgcaagcgt
     2701 tcgccgagcg gctgcaggct ttcctgtcgg cgctgccgcg gggaccgctg tacgccgtgg
     2761 agctgcgcga ccacgtgctg ctgacgccgg cctaccgtga tgcgctggcg gcctcgggcg
     2821 cccatcactg cctgggcctg cacccgcgca tgccggtcgc ctcggagcag gcgcgcgaaa
     2881 ccgcgctgga ccacagcgga ccgctgatcg tgcgctggaa tctgcacgcg ggcttcggct
     2941 acgaggaggc gagggcgcgc tatgcgccgt tcgcgcggct ggtggacgag gacatcccca
     3001 accgcgtgtc gctcgcccgg ctgtgcctgg acgccgccgc tcgccgtgag cccgtgttcg
     3061 tgatcgccaa caacaaggcc gagggatcgg cgccgctgac cctgatccgg ctggcggagc
     3121 gcatcgtggc acacctgccc aagcgccatt gatcgcgccg ggttaccatc cgcgcgatct
     3181 gcacgacatg gatagcgaca tgaagaaacc gaccaggatc gatcccgagg actcgatgga
     3241 agaccaactc aaagcggctg cgctcgagta ccacacccaa cccacgcccg gcaagatctc
     3301 ggtggtgccg accaaggggc tgaccaacca gcgcgacctg gcgctcgcgt attcgcccgg
     3361 cgtggcgtac gcgtgcaagg cgatcgaggc cgatccggcg caggccgcgc gcttgacctc
     3421 gcgcggcaac ctggtgggcg tcatcaccaa cggcaccgcg gtgct
//
LOCUS       DASPGE010000352         4390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1378051, whole genome shotgun sequence.
ACCESSION   DASPGE010000352 DASPGE010000000
VERSION     DASPGE010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378051"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VD791_11370"
     CDS             complement(<1..274)
                     /locus_tag="VD791_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08622.1"
                     /translation="MKSFHKPAGFAFAACAAALAGIMARGVPAQQLDISQTPLASASD
                     LAVLPNLLFLLDDSGSMMFDTLPDHTERVQGGNERRYWHRNFNCKPK"
     gene            complement(455..1042)
                     /locus_tag="VD791_11375"
     CDS             complement(455..1042)
                     /locus_tag="VD791_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08623.1"
                     /translation="MTKPSFPSPRGQSGAVLFIALIVLVAMTLAGIAIMRSVDTGNQI
                     AGNVAFKQGTLTSADRGFDVAFDFLKNNVDTALLDNDFPAEAYYASVSRDPDVDPPDW
                     SNATTWTDAKVVGTDAAGNTISYLIHRMCYDIGSYQGQYCATTKKSGGAAGNSQTVGG
                     TNYQPIPMVYFRVTVRVQGPRDTMSMVQSNVGVQF"
     gene            complement(1039..2226)
                     /locus_tag="VD791_11380"
     CDS             complement(1039..2226)
                     /locus_tag="VD791_11380"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HXV08624.1"
                     /translation="MQRQTLHKDQLGMSIVELMVAVAISLIGVLVIFQVFAVNEGVRR
                     STTGGSDEQTSGLLGLMLLERELRHAGFGINDPSLVGCNMRVYDNMRAPVMQPDFPLT
                     PVEITSNAGVVPDVIKVVYGGVTQTTAAVPFSVTADDPTEPIQLTFRFGFNNGDVMVV
                     GQESLNCTLREVTGPYPLLLGVVELEHGVGAFTNAYTKNSQTPRFNNPAGTPDLYAKD
                     VGKVMNLGPFPVRNEITVINNDPDPSRNNQLVGQNVWAQMAGFQPLADQIVHLKAEYG
                     MDDGISNGTVLRPVYNAGDNVVDKFTPVSPATPADWRNVLSVRLAIVSRTNIIEKPVV
                     GPGCDATPDWGTPEYPVLWARGPDAPQGRPIDVRVTPNWRCFKYRVYETTVPLRNMLW
                     RQG"
     gene            complement(2238..2702)
                     /locus_tag="VD791_11385"
     CDS             complement(2238..2702)
                     /locus_tag="VD791_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08625.1"
                     /translation="MKHSRIRRPLPRPLRAAHQAGVMLLEALIALLIFSLGILAIVGM
                     QATAIQDMGEAKYRSDAAFLANQVIADMWSNSAQLNTYTWGGAGAPPAQIQNWVSTVY
                     NRLPGSSDFPPIIAVGANNMVTVTVRWQQARDKSTAAPPHRHTSVAYITCCL"
     gene            complement(2725..3282)
                     /locus_tag="VD791_11390"
     CDS             complement(2725..3282)
                     /locus_tag="VD791_11390"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HXV08626.1"
                     /translation="MLIASTAQPGGRRRGQRGVTLIELMLALVVLAVLLTLGVPSFQA
                     WLQNTQIRTATDALMNGLQMARTEAVRRNTIVYFALETGSNWTVTQFNPKQVLQERRG
                     DEGSQTAVIDSGGRTMVSFSPIGAPMATNPEDGSLPIATINVTSAVTVEAVRPLRIVI
                     SPSGAVRMCDPDPNLPAGDPRRCTI"
     gene            complement(3276..3695)
                     /locus_tag="VD791_11395"
     CDS             complement(3276..3695)
                     /locus_tag="VD791_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HXV08627.1"
                     /translation="MNSQTGFTLLEVMITVAIIGILAAIAIPAYNDYVLRGQLSEAYA
                     QLAAQRVRMEQYYQDMRTYTGAEAPGTVATCPGPGQHFTYACAVDAGGQGYTISATGR
                     GFTFSIDQNNNRMTTAVPADWALPATNCWVRKKGGVC"
     gene            4069..>4390
                     /locus_tag="VD791_11400"
     CDS             4069..>4390
                     /locus_tag="VD791_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HXV08628.1"
                     /translation="MDLTQYTRDNLAEVLRRHGINPTHQRIEIAHALYSRHEHLSADQ
                     VLALVNERHPETSRATVYNTLNLFVEKRLIREVIVDPNRVFYDPNTAPHYHLYDVRTG
                     RLTDI"
BASE COUNT          689 a         1425 c         1499 g          777 t
ORIGIN      
        1 ccttcggctt gcagttgaag ttgcgatgcc agtagcgccg ttcgttgccg ccctgcacgc
       61 gctcggtgtg atcgggcagc gtgtcgaaca tcatcgagcc cgagtcgtcg agcaggaaca
      121 ggagattggg caggaccgcc agatccgagg cgctggcgag cggcgtctgc gagatgtcga
      181 gctgctgggc ggggacgccg cgcgccatga tgccggcaag cgcggcggcg caggccgcga
      241 acgcgaagcc tgcgggtttg tgaaacgact tcatagcgga cctcctcccc gtccaagcta
      301 cgatcaaaca cggggcggtc tccacgcccc gctgtcaaag ctacaacgct ttgaaggtac
      361 aacaccgggg agccgtctcc aagcccctcg gcgtcgcacg tcctagcgca ttgtcaaatc
      421 cgcggccgct cgatcgcgcg ctcgcgttgc atgctcagaa ctgcacgcct acgttcgact
      481 gcaccatgct catggtgtcg cgcggtcctt gcacgcgcac cgtcaccctg aagtacacca
      541 tcggaatcgg ctgatagttg gtgccgccga cggtctggct gttgcccgcc gcgccgccgc
      601 tcttcttggt ggtggcgcag tactggccct gatagctgcc gatgtcgtag cacatgcgat
      661 ggatcagata ggagatggtg ttgcccgcgg cgtccgtgcc cacgaccttg gcgtccgtcc
      721 aggtggtcgc gttgctccaa tcgggcggat cgacgtccgg atcgcgcgac accgatgcgt
      781 aataggcctc ggcgggaaag tcgttgtcca gcagcgcggt gtccacgttg ttcttgagga
      841 agtcgaatgc cacgtcgaag ccgcgatcgg cggaggtcag cgtgccctgc ttgaaggcca
      901 cgttgccggc gatctggttg ccggtgtcca ccgagcgcat gatcgcgatg ccggcgagcg
      961 tcatcgcgac cagcacgatg agggcgatga acaggacggc gccggactgc ccgcgcggcg
     1021 acgggaacga tggcttggtc atccttgcct ccaaagcatg ttgcgcaggg gcacggtggt
     1081 ttcgtacacc ctgtacttga agcaacgcca gttgggcgtg acgcgcacgt cgatcggacg
     1141 cccctgcgga gcgtcggggc cgcgcgccca gagcaccggg tactcgggcg tgccccagtc
     1201 gggggtggcg tcgcagcccg gcccgacgac gggtttctcg atgatgttgg tgcgcgacac
     1261 gatcgccagg cgcacgctca gcacgttgcg ccagtcggcc ggagtggccg ggctgaccgg
     1321 cgtgaacttg tccaccacat tgtcccccgc gttgtacacc ggccgcagca cggtgccgtt
     1381 ggagatgccg tcgtccatgc cgtactcggc cttgaggtgc acgatctgat cggccagcgg
     1441 ctggaagccc gccatctgcg cccacacgtt ctggcccacc agctgattgt tccgggaagg
     1501 atcggggtcg ttgttgatca ccgtgatctc gttgcgcacc gggaacgggc cgaggttcat
     1561 caccttgccg acgtccttgg cgtacagatc cggagtgcct gcggggttgt tgaagcgcgg
     1621 cgtctgcgag ttcttggtgt aggcgttggt gaacgcgccc acgccgtgct cgagctcgac
     1681 cacgcccagc agcagcggat aggggccggt cacctcgcgc agcgtgcaat tcaggctctc
     1741 ctggcccacc accatgacat cgccgttgtt gaagccgaag cggaacgtga gctggatcgg
     1801 ctcggtgggg tcgtccgcgg tcacgctgaa cggcaccgcc gcggtggtct gggtaacccc
     1861 gccgtacacc accttgatca cgtccggcac gacgcccgcg ttcgaggtta tttcgaccgg
     1921 cgtcaacggg aagtccggct gcatcaccgg cgcgcgcatg ttgtcgtaga cgcgcatgtt
     1981 gcagcccacc aggcttggat cgttgatgcc gaatccggcg tggcgcagct cgcgctcgag
     2041 cagcatcagg ccgagcaggc cgctggtctg ctcgtcgctg ccgccggtgg tgctgcggcg
     2101 tacgccctcg ttcacggcga acacctggaa gatgaccagc acgccgatca gcgagatcgc
     2161 gacggccacc atcagctcga cgatgctcat gccgagctgg tctttgtgga gagtctgccg
     2221 ttgcatgggg tgcgctccta caggcagcag gtgatgtagg ccacgctggt gtggcggtgc
     2281 ggcggcgcgg ccgtgctctt gtcgcgcgcc tgctgccagc gcaccgtcac cgtgaccatg
     2341 ttgttcgcgc ccacggcgat gatcggcgga aagtcgctgg agccgggcag ccggttgtac
     2401 acggtgctca cccaattctg gatctgtgcc gggggcgcgc cggcgccgcc ccaggtgtag
     2461 gtgttgagct gcgcggagtt gctccacatg tcggcgatca cctggttggc caggaaggcc
     2521 gcgtcgctgc ggtacttggc ctcgcccatg tcctggatgg cggtggcctg catgccgacg
     2581 atggcgagga tgccgagcga gaagatcagc agcgcgatca gcgcttcgag cagcatgacg
     2641 ccggcctggt gcgcggcgcg cagcgggcgc ggcagagggc ggcggattcg ggagtgtttc
     2701 atcggcgtgc ctggttcgtc gggttcagat ggtgcagcgc cgcgggtcgc ccgcgggcag
     2761 gttgggatcg gggtcgcaca tgcgcacggc gcccgagggc gagatcacga tgcgtagcgg
     2821 ccgcaccgcc tccaccgtga ctgcgctggt gacgttgatg gtggcgatgg gcagcgaacc
     2881 gtcctccgga ttggtagcca tcggcgcgcc gatcgggctg aagctgacca tggtgcgccc
     2941 gccgctgtcg atgacagcgg tctgcgaccc ttcgtcgccg cggcgctcct gcagcacctg
     3001 cttcgggttg aactgcgtca cggtccagtt gctgccggtc tccagcgcga agtagacgat
     3061 cgtgttgcgg cgcacggcct cggtgcgtgc catctgcagg ccgttcatca gggcgtcggt
     3121 cgcggtgcgg atctgcgtgt tctgcagcca ggcctggaac gaaggcacgc ccagggtcag
     3181 cagcacggcg agcaccacca gcgcgagcat cagctcgatc agggtcacgc cgcgctgccc
     3241 gcggcgccgc ccgccgggtt gcgcggtgct tgcgatcagc atacgcctcc cttcttgcgc
     3301 acccagcagt tggtggcagg caaggcccag tcggccggca ccgcggtggt catccgattg
     3361 ttgttctggt cgatgctgaa cgtgaaaccg cgacccgtgg cgctgatggt gtagccctgg
     3421 ccgccggcgt ccaccgcgca ggcataggtg aagtgctggc ccgggcccgg gcacgtcgcc
     3481 acggtgccgg gcgcctctgc gccggtgtaa gtgcgcatgt cctggtagta ctgctccatg
     3541 cgcacccgtt gcgcggcgag ctgggcgtag gcttcgctga gttgaccccg caggacgtag
     3601 tcgttgtatg ccggaatggc gatggccgcg aggatcccaa tgatcgccac ggtgatcatc
     3661 acctccagca acgtgaatcc tgtctgcgaa ttcatcgaaa cggctccttt acccctcggg
     3721 gtgagatcga tattaataaa gagaatcctg tacttgcaaa aaccgccgac caatggtcgc
     3781 ctggccacga cgagcgggcg ccggcgtcac cggccggttc acccggggcc acgaacggtc
     3841 gccggcggtt gcatcggccg gcgcggaggt ttaccccgca ccgacggtcg ttgtgggcgg
     3901 gtgcaagacc ggtgccagcg agtgctcgcc tgcctggccg cttggacaac cccgccgcac
     3961 tggccgcgcc gggcttcaag ctggcgggat gcggcatccc cgcgcccggc gtccaggcgc
     4021 cagatgcgaa ttagacacag tccaactcgc tggtctatac ttgcgctcat ggatctcacg
     4081 caatacaccc gcgacaatct cgccgaggtg ctgcggcgcc acggcatcaa cccgacgcac
     4141 cagcgcatcg agatcgcgca cgcgctgtat tcgcgccacg agcatctgtc cgcggaccag
     4201 gtgctggcgc tggtgaacga gcgccatccg gagacctcgc gggcgaccgt ctataacacg
     4261 ctcaacctgt tcgtggagaa gcggctgatc cgcgaagtga tcgtggatcc gaaccgggtg
     4321 ttctacgacc cgaacaccgc cccgcactat cacctgtacg acgtgcgcac cggccgcctg
     4381 accgacatcg
//
LOCUS       DASPGE010000353         8399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_443786, whole genome shotgun sequence.
ACCESSION   DASPGE010000353 DASPGE010000000
VERSION     DASPGE010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443786"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VD791_11405"
     CDS             <1..370
                     /locus_tag="VD791_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXV08629.1"
                     /translation="SSGPAVVSSDPAVVSSDLAPSPGSSARPAASAAAPSKAQEAARA
                     GGETYAVALIATASPAKAAQMKRALQRQKFPVYTQKTPDGDKTRVRVGPFASREAAEQ
                     ARQRLVRQGYDPGKVVRAGE"
     gene            417..908
                     /locus_tag="VD791_11410"
     CDS             417..908
                     /locus_tag="VD791_11410"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HXV08630.1"
                     /translation="MTLLDLAALLVVALSVLISVVRGAMREVMSIVSWVASVLLAVYL
                     APAAATLLPAQLANPGLRLAVAFAVILLVSLLLFGLLALSLSKLVRKSGLSASDRALG
                     ALFGLARGVVILVVLTLLAGLTRLPREPAWRDAMLRPPLEALALAARDYLPQALASRI
                     GYD"
     gene            993..2522
                     /gene="purF"
                     /locus_tag="VD791_11415"
     CDS             993..2522
                     /gene="purF"
                     /locus_tag="VD791_11415"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXV08631.1"
                     /translation="MCGIIGVVAKSPVNQLLYDGLQVLQHRGQDAAGIVTAAESAFHM
                     HKGSGLVRDVFRTRNMRMLMGTTGIGHCRYPTAGSAENSAEAQPLYVNSPFGIVLGHN
                     GNLTNSEQLTRELFRQDLRHVNTNSDSEVLLNVLAHELQDASSSYALDPATIFQAVAG
                     VHRRCRGAYAVVAMIAGYGLLAFRDPYGIRPLVVGMSRSEQGDEYLVASESVALDVLG
                     FKLVRDVQPGEAIFIDMDGNFYSKQCAVNPSLNPCIFEFVYLARPDSVIDGISVYETR
                     LRMGESLAEKIAREHADLAIDVVIPIPDSSRPSAMQLARRLNLEYREGFIKNRYIGRT
                     FIMPGQSVRKRSVRQKLNAIGMEFKGKNVLLVDDSIVRGTTSREIVQMARDAGARQVF
                     FASASPPVRFPNVYGIDMPSSKELIANGRSPQEVAREIGADCLIYQDLDALIEDARSV
                     NPRVQSFETSCFSGMYITGDVTAEYLADIEASRSDSELMRRAAVAMATTQLDLNLAEA
                     E"
     gene            complement(2606..3256)
                     /locus_tag="VD791_11420"
     CDS             complement(2606..3256)
                     /locus_tag="VD791_11420"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HXV08632.1"
                     /translation="MFFELAIVVGTVAGTLLIQRTLRGSRGRPSSRLRTLREPTAGDD
                     FQALLAAHPPYPAAVREPVKPVPVPAAGGGAAENDAGPPRFLQGSAKVLYLVLKAALP
                     EYHVFAYARLADVLKRIGRRLPSQTRAQLGQSRLDFVICNKDLVLVALLDLSDGTKAD
                     DSAKQQLQAPLSAAGIRYVRVAPNAIPKPSEVRSLVLGGYADFCPDSTQIRLRQLP"
     regulatory      3468..3542
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            3616..4785
                     /locus_tag="VD791_11425"
     CDS             3616..4785
                     /locus_tag="VD791_11425"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HXV08633.1"
                     /translation="MTDLDFETLAVRAGIHRSQFNEHAEALYLTSSFVFDNAAQAAAR
                     FSGAEAGMVYSRYTNPTVQMLQERLAALEAAECCVATASGMSAILATVLGLLRAGDHI
                     VAAQTLFGATVQLFNNWLPRFGIQTTLVAGADPEVWGAALRPNTRMLFLETPSNPLTE
                     IYDISAFAQLAHRKDALLAVDNCFCTPALQRPLALGADLVIHSATKYLDGQGRVLGGA
                     VLGARALIEEPLTTFLRNAGPTLSPFNAWVILKGLETLKLRMDAHSAGALELARWLEA
                     HPRVERVYYPGLPSHPQHALAARQQRTGGGIVSFVVKGGREGAWHVVDSTRLISITAN
                     LGDAKSTITHPASTTHGRLSPEARAAAGVEEGLLRVAMGLESVRDIETDLARGLG"
     gene            complement(4813..5541)
                     /locus_tag="VD791_11430"
     CDS             complement(4813..5541)
                     /locus_tag="VD791_11430"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HXV08634.1"
                     /translation="MFTAFVSDLHLTAARPPATRAFFAFLDGVAAQADALYILGDLFE
                     YWAGDDDLADPFNREVAQAIKAVSARGVPVRVIRGNRDFLLLERFAGAAEVNLVQDPA
                     LVDLYGTPTLLMHGDLLCTQDWRYQMFRKVVRNRGVQRWFMRLPLDQRKRVIGGARRM
                     SEGEKQAKKAQIMDVTPAAVVEAFRAHGCARLIHGHTHRPARHEHAVDGRACERWVLS
                     DWYERGQYLRVTPAGCTPVDLPFA"
     gene            complement(5579..6130)
                     /locus_tag="VD791_11435"
     CDS             complement(5579..6130)
                     /locus_tag="VD791_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV08635.1"
                     /translation="MPTQRIATLAAWLLCGALRLAPALADEYRDLYQLYKQGDTAQAL
                     ERIDAYLASQPRDARARFLKGVILTDQKRRDEAITVFTGLTQDFPELPEPYNNLSVLY
                     AAQGDYERAREVLEMAVRNYPGYATAHENLGDVYAMLASQAYDKAVRADPKNTTAPIK
                     LRMVRELFSVKAPPPEAAPGANR"
     gene            6299..7672
                     /gene="cysS"
                     /locus_tag="VD791_11440"
     CDS             6299..7672
                     /gene="cysS"
                     /locus_tag="VD791_11440"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HXV08636.1"
                     /translation="MLHIHNSLTRQKQAFVPLQPGKVRMYVCGMTVYDYCHIGHARVM
                     VVFDVVRRWLVSSGFDVTYVRNITDIDDRIIKRAQDNGEPFQALTERFIRYMDEDVAA
                     LGVEKPDFEPRATQYVPDMLRIIEMLMRKGLAYHAANGDVYYSVRGFEGYGKLSGKSL
                     EDLRAGERVEVDPNKRDPMDFVLWKAAKPGEPTWESPWGPGRPGWHIECSAMSEHFLG
                     EHFDIHGGGQDLLFPHHENEIAQSEGAHGHAFVNYWVHNGFVRVDNEKMSKSLGNFFT
                     IREVLAKYDPEVVRFFIVRAHYRSPLNYSDQHLEDARAGLTRFYTTLRDTPPARAERI
                     DWANPYAARFRQAMDDDFNTAEATAVLFELASEVNRTRSSADAGLLRALAAILGLLER
                     DPVAFLQAGSGGELTPAAIEALIAQRTAARKAKNFAEADRIRKELQSKGVLLEDTPDG
                     RTIWRRK"
     gene            7795..8235
                     /locus_tag="VD791_11445"
     CDS             7795..8235
                     /locus_tag="VD791_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08637.1"
                     /translation="MDLSGYGYRRAQRTTSSTTNDTGSSTFNDTGRVTSNNTASATAA
                     TTASGSTSELSAEAVRLLASLGKRVSMNELAAQYPRVLNRIAAVWYKRAEADRCFDEL
                     LLDARRTRQGFPQSVVYEIASLRHHHVTHVFPKHVDPWEQSQLR"
BASE COUNT         1309 a         2881 c         2899 g         1310 t
ORIGIN      
        1 gtcctccggc ccggcggtgg tttcctccga ccccgcggta gtttcctcgg acttggcgcc
       61 gagtcccgga tcgtccgcga ggccggccgc gtcggcggcc gcaccgagca aggcccagga
      121 ggcggcgcgt gccggcggcg aaacctatgc ggtggcgctg atcgccaccg cctcacccgc
      181 caaggcggcg cagatgaagc gcgcactgca acgccagaaa ttcccggtct acacgcagaa
      241 gacgccggac ggcgacaaga cgcgggtgcg ggtggggccg ttcgccagcc gcgaagcggc
      301 ggagcaggcg cgccagcggc tggtccggca gggctacgac cccggcaagg tggtgagggc
      361 gggggagtga cgcggcccac ccgctgcatg ctcggcccgg cgctgcgggg ggatcggtga
      421 ctctgctcga tctcgcggcg ctcctggtag tcgcgctgtc ggtgttgatc agcgtggtgc
      481 gcggggcgat gcgcgaggtg atgtcgatcg tctcctgggt cgcctcggta ttgctcgcgg
      541 tgtacctggc gccggcggcc gcaacgctgc tgcccgcgca gctggccaac cccggcctgc
      601 gcctggcggt cgcgttcgcg gtgatcctgc tggtgagctt gctgctgttc gggctgctcg
      661 cgttgagcct gtccaagctg gtgcgcaaat cgggactgtc ggccagcgac cgcgcgctcg
      721 gtgcgctgtt cgggctggcg cgcggggtgg tgatcctggt ggtgctcacg ctgctggcgg
      781 ggttgacgcg gctgccgcgc gaaccggcgt ggcgcgacgc catgctccgt ccgccgctcg
      841 aggcgctggc gctggccgcg cgcgattacc tgccgcaggc gctcgcgtcc cggatcggct
      901 acgattgacg gggccagtcg cgtcgagcgg gccgggtacg gttgagcgcg cgcgaagcgc
      961 cttcatagtg agcttctcat cggaggccgg caatgtgcgg aatcatcggt gtagtcgcca
     1021 agtcccccgt caaccagctg ctctacgacg ggctgcaggt cctccagcac cgtggccagg
     1081 acgccgccgg gatcgtcacc gccgccgaga gcgcgttcca catgcacaag ggcagcggtc
     1141 tggtgcgcga cgtgttccgc acgcgcaaca tgcgcatgct gatgggcacc accggcatcg
     1201 gccattgccg ctaccccacc gccggctcgg ccgagaactc ggccgaagcg cagccgctgt
     1261 acgtcaactc gccgttcggc atcgtcctcg ggcacaacgg caacctgacc aacagcgaac
     1321 agctcacgcg cgagctgttc cgccaggacc tgcgccacgt caacaccaac tccgactcgg
     1381 aggtgctgct caacgtgctg gcccacgagc tgcaggacgc ctccagctcg tacgcgctcg
     1441 acccggccac cattttccag gcggtggcgg gcgtgcaccg gcgctgccgc ggcgcctacg
     1501 cggtggtggc gatgatcgcg ggctacggcc tgctcgcgtt ccgcgacccc tacggcatcc
     1561 gcccgctggt ggtgggcatg tcgcgcagcg agcagggcga cgagtacctg gtggcttccg
     1621 agagcgtggc gctggacgtg ctggggttca agctggtgcg cgacgtgcag ccgggtgagg
     1681 ccatcttcat cgacatggac ggcaacttct acagcaagca gtgcgccgtc aatccctcgc
     1741 tcaacccctg catcttcgag ttcgtgtacc tggcgcgccc cgactcggtg atcgacggca
     1801 tctcggtgta cgagacgcgg ctgcgcatgg gcgagagcct ggccgagaag atcgcgcgcg
     1861 agcacgccga cctggcgatc gacgtggtga tcccgatccc ggactcgagc cgcccttcgg
     1921 ccatgcagct cgcgcgccgg ctgaacctcg agtaccgcga gggcttcatc aagaaccgct
     1981 acatcggccg caccttcatc atgcccggcc agtcggtgcg caagaggtcg gtgcgccaga
     2041 agctgaacgc catcggcatg gagttcaagg gcaagaacgt gctgctggtg gacgactcga
     2101 tcgtgcgcgg caccaccagc cgcgagatcg tgcagatggc gcgggacgcc ggcgccaggc
     2161 aggtgttctt cgcgtcggcc tcgccgccag tgcgcttccc caacgtgtac ggcatcgaca
     2221 tgcccagctc caaggagctg atcgcaaacg gccgctcccc ccaggaggtc gcgcgcgaga
     2281 tcggcgccga ctgcctgatc taccaggacc tcgacgcgct gatcgaggac gcgcgctcgg
     2341 tcaaccccag ggtgcagagc ttcgagacct cgtgcttcag cggcatgtac atcacgggcg
     2401 atgtgaccgc cgagtatctc gccgacatcg aggcgagccg cagcgattcc gagctgatgc
     2461 gccgcgcggc ggtggccatg gccaccacgc agctggatct caatctggcc gaggcggagt
     2521 aggcggcgcc gttcgtacgg ttttggggcg taccggccca ggtggtgtac cggcctggcc
     2581 cgccgttgcc gaaacgcgga ttaggctagg gcagctgccg caggcggatc tgcgtcgagt
     2641 ccgggcagaa gtccgcatag cccccgagaa ccagcgaacg cacttccgag ggcttgggga
     2701 tggcgttggg cgccacgcgc acgtagcgga ttccggcggc cgaaagcggc gcttgcagct
     2761 gctgcttggc ggaatcgtcg gccttggtgc cgtcgctcag gtcgagcagg gcgaccagca
     2821 cgagatcctt gttgcagatc acgaaatcca ggcgcgactg cccgagctgt gcgcgcgtct
     2881 gggacggcag gcgcctgccg atgcgcttga gcacatcggc gagccgggcg taggcgaaga
     2941 cgtgatactc cggcaacgcc gccttcagca ccagatagag caccttggcc gaaccctgca
     3001 gaaagcgggg aggccccgca tcgttctcgg cggcgccccc gcccgcagcg gggaccggga
     3061 ccggcttgac cggctcccgg acggcggccg ggtagggcgg gtgggcggcg agcagcgcct
     3121 gaaagtcatc cccggccgtg ggctcgcgca gcgtgcgcag gcggctcgac ggcctcccgc
     3181 ggctgcctcg cagggtgcgc tggatcagaa gcgttccggc taccgtgccg acgacgatgg
     3241 cgagttcgaa aaacatggcc caaggcgtga gatccccggg aacgcgagca accggcgtgc
     3301 ccgttttggc ccgagggacc gagatttcgg cgcccgcctg acggcgcgag aaacgcgcca
     3361 aagcgctctc tcgatcgaac ccctcccctc ccccggggtt gacagcaacg cgcccgggta
     3421 gacagcaacg cccccgggtt gacagcaacg caaggcgcgc gtagtgtcct cgctgtcgcg
     3481 ccgatttgag gctacagctt gcgggcgcgt taaaaatgca gctaaagcgt gggcagaacc
     3541 cgcgacggcc ccgctgcgcg ggttttttgt ttgaagcggg ctgcgcgggt tttttgtttt
     3601 cggggcttgc gagatatgac ggacctggac ttcgagacgc tggcggtgcg cgcaggcatc
     3661 caccgcagcc agttcaacga gcacgccgag gcgctgtacc tcacctcgag cttcgtattc
     3721 gacaacgcgg cgcaggccgc ggcgcgcttc tccggcgccg aggccggcat ggtgtactcg
     3781 cgctatacca acccgaccgt gcagatgttg caggagcgtc tggccgcgct ggaggcggcc
     3841 gagtgctgcg tggccaccgc gtcggggatg tccgcgatcc tggccacggt gctgggcctg
     3901 ctgcgcgccg gcgatcacat cgtcgccgcg cagacgctgt tcggcgccac cgtgcagctg
     3961 ttcaacaact ggctgccgcg cttcggcatc cagaccacgc tggtggcggg cgccgacccg
     4021 gaagtgtggg gggcggcgct gcgccccaac acgcgcatgc tgttcctgga gacgccttcc
     4081 aacccgctga ccgagatcta cgacatctct gctttcgcgc agctcgcgca ccggaaagac
     4141 gcactgctgg cggtggacaa ctgcttctgc acgccggcgc tgcagcgccc gctggcgctg
     4201 ggtgcggacc tcgtgatcca ttcggcgacc aagtacctgg acgggcaggg ccgggtgctg
     4261 ggcggcgccg tgctcggcgc gcgcgcgctg atcgaggagc cgctcaccac tttcctgcgc
     4321 aatgccggcc ccacgctgtc gcccttcaat gcctgggtca tcctcaaggg gctggagacg
     4381 ctcaagctgc gcatggatgc gcactcggcg ggcgcgctcg agctggcgcg ctggctcgaa
     4441 gcgcacccgc gcgtggagcg cgtgtactat ccgggcttgc cgagccatcc gcagcacgcg
     4501 ctggccgcgc gccagcagcg caccggcggc ggcatcgtct ccttcgtggt caagggtggg
     4561 cgcgagggcg cctggcacgt ggtcgattcc acccggctca tctcgatcac cgccaatctt
     4621 ggcgacgcca aatccaccat cacgcatccg gccagcacca cgcacgggcg cctttcgccg
     4681 gaggcgcgcg cggccgccgg cgtggaggaa gggttgctgc gggttgcgat gggcctggag
     4741 tcggtgcggg acatcgagac cgatctcgcg cgcggattgg gctagcccgg atatttcacc
     4801 gggcgggcta gctcacgcga agggcaggtc caccggcgtg cagcctgcgg gcgtgacgcg
     4861 caggtactgc ccgcgctcgt accagtccga cagcacccag cgctcgcacg cgcgcccgtc
     4921 cacggcgtgc tcgtgccgcg ccggccggtg ggtgtggccg tggatcaagc gcgcgcagcc
     4981 gtgcgcgcga aacgcctcga ccaccgccgc cggcgtgacg tccatgatct gggccttctt
     5041 ggcctgcttc tcgccctcgc tcatgcggcg cgcgccgccg atcacgcgct tgcgctggtc
     5101 cagcggcagg cgcatgaacc agcgctgcac cccgcggttg cgcacgacct tgcggaacat
     5161 ctgatagcgc cagtcctgcg tgcacagcag gtcgccgtgc atcagcagcg tcggcgtgcc
     5221 gtagaggtcg accagcgccg ggtcctgcac caggttcacc tcggccgcgc ccgcgaaccg
     5281 ctccaggagc aggaagtcgc ggttgccgcg gatcactcgc accggcacgc cgcgcgcgct
     5341 caccgctttg atggcctgcg ccacttcgcg gttgaacgga tcggcgagat cgtcgtcacc
     5401 ggcccaatac tcgaacaggt cgcccaggat gtagagcgcg tccgcctgcg cggcgacacc
     5461 gtccaggaag gcgaaaaacg cgcgcgtggc cggcgggcgc gcggccgtca ggtgcaggtc
     5521 cgagacgaaa gcggtgaaca tcaacggcct gatgcgccac cgccgagccg gccgcggctc
     5581 atcgattcgc gcccggggcg gcctcgggcg gcggcgcctt caccgagaac agctcgcgta
     5641 ccattcgcag cttgatcggc gcggtggtgt tcttcggatc ggcccgcacg gctttgtcgt
     5701 aggcctggct cgccagcatg gcgtacacgt cgccgagatt ctcgtgcgcg gtcgcatagc
     5761 ccgggtagtt gcgcaccgcc atctccagca cctcgcgcgc gcgctcgtag tcgccttgcg
     5821 ccgcgtacag caccgagagg ttgttgtacg gctcgggcag ctcggggaag tcctgggtca
     5881 gcccggtgaa caccgtgatc gcctcgtcgc ggcgcttctg gtcggtgagg atcaccccct
     5941 tgagaaaacg cgcccgggca tcgcgcggct ggctggcgag gtaagcgtcg atgcgctcca
     6001 gcgcctgggc ggtgtcgccc tgcttgtaga gctggtacag gtcccgatac tcgtcggcaa
     6061 gcgccggcgc gagccggagc gcaccgcaca gaagccaggc tgcgagcgtc gcgatgcgct
     6121 gcgtgggcat tgtgctatac tcgattcggg ttgcgctagg ttattgttct cctggcgatt
     6181 ttagcaagga cacgggcgct tccacaatca cccctgcgct tctacagtca tcgggcgccg
     6241 gcccgccgcg gccggtgcac gaggcaggtg agaggcgctg cacgcgggcc cgttcccgat
     6301 gctacacatc cacaactcgc tcacgcgcca gaagcaggcg ttcgtcccgc tgcagcccgg
     6361 caaggtgcgc atgtacgtct gcggcatgac ggtgtacgac tactgccaca tcgggcatgc
     6421 gcgcgtcatg gtggtgttcg acgtggtgcg ccgctggctg gtgagctccg gcttcgacgt
     6481 cacctacgtg cgcaacatca ccgacatcga cgaccggatc atcaagcgtg cgcaggacaa
     6541 cggcgagccg ttccaggcgc tcaccgagcg cttcatccgc tacatggacg aggatgtcgc
     6601 ggcgctgggc gtggagaagc cggatttcga gccgcgcgcg acccagtacg tgcccgacat
     6661 gctgcgcatc atcgagatgc tgatgcgcaa ggggctcgcc taccacgccg ccaacggcga
     6721 cgtgtactac tcggtgcgcg gcttcgaggg ctacggcaag ctttccggca agtcgctgga
     6781 ggacctgcgc gcgggcgagc gcgtggaggt ggacccgaac aagcgcgacc cgatggactt
     6841 cgtgctgtgg aaggcggcca agccgggcga gccgacctgg gaatcgccct ggggaccggg
     6901 gcgccccggc tggcacatcg agtgctcggc catgagcgaa catttcctgg gcgagcactt
     6961 cgacatccac ggcggcggcc aggacctgct gttcccgcac cacgagaacg agatcgccca
     7021 gtccgaaggc gcgcacggcc atgccttcgt caactactgg gtgcataacg gcttcgtgcg
     7081 cgtggacaac gagaagatgt ccaagtcgct gggaaacttc tttaccatcc gcgaggtgct
     7141 ggcgaagtac gaccccgagg tggtgcgctt cttcatcgtg cgcgcccact accgcagccc
     7201 gctcaactat tccgaccagc atctggaaga cgcgcgcgcc gggctcacgc ggttctacac
     7261 cacgctgcgc gacacgccgc ccgcgcgcgc ggagcgcatc gattgggcga acccctacgc
     7321 cgcgcgtttc cggcaggcga tggacgacga cttcaacacg gcggaagcca ccgcggtcct
     7381 gttcgagctg gcgagcgagg tgaaccgcac gcgctcgtcc gcggatgccg ggctgctgcg
     7441 cgcgctcgcc gccatcctcg gcctgctcga gcgcgacccg gtggcgttcc tgcaggccgg
     7501 ctcgggcggc gagttgacgc ccgcggcgat cgaggcgctg atcgcgcagc gcaccgccgc
     7561 acgcaaggcg aagaacttcg ccgaggcgga ccgcatccgc aaggagctgc agagcaaggg
     7621 cgtgctgctg gaggacactc ccgacgggcg cacgatctgg cggcggaagt agcagcgaag
     7681 cgtcgtctcg aagcgactcg cggtggaatg tgaacgccgg tttaccgtgg ttgacgcccg
     7741 tgcgccgtga ctgcaatctg cggcatcgcc acagctcgca cccccttctc accgatggat
     7801 ctcagcggtt acggttatcg acgcgcgcag cgcaccacgt cctcgactac caacgacacg
     7861 gggtcctcga ctttcaacga cacggggcgc gtcacttcca acaacacagc atccgcaacc
     7921 gccgcgacca ccgcgagcgg ttccacgagc gagctcagcg ccgaggcggt tcgcttgctg
     7981 gccagcctgg gcaagagagt gtccatgaac gagctcgccg cgcaatatcc gcgggtgctg
     8041 aatcgaatcg ccgcggtgtg gtacaagcgc gccgaggccg atcgctgctt cgacgaactg
     8101 ctgctcgacg cgcggcgcac gcgtcaaggt ttcccgcaat ccgtggtcta cgagatcgcg
     8161 tccctgcgcc accaccacgt cacgcacgtg ttccccaagc acgtggatcc gtgggagcaa
     8221 agccaactcc gttagcgggc cgcgcgcacc gcgcaccgcg cctagcccgg atatttcacc
     8281 aggccgcccc cgcatcgcat gcgtaggcgc accagcaaac gtttctaccg cgtgtgctca
     8341 gcggtgcatc caagggcttc ggcttcgtgg agatgccggg caaggcgcat tcgctggcc
//
LOCUS       DASPGE010000354         2521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1027644, whole genome shotgun sequence.
ACCESSION   DASPGE010000354 DASPGE010000000
VERSION     DASPGE010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027644"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VD791_11450"
     CDS             complement(<1..300)
                     /locus_tag="VD791_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXV08638.1"
                     /translation="MGAVQSTRDGLAPQPDELSGIVLEQADRIFAGEVTAERLAAADR
                     GEWPHAIWDAVLNAGLTLALVPEAQGGVGLAAADGLRLMRRAGYHTVPLPLPETMV"
     gene            complement(300..1451)
                     /locus_tag="VD791_11455"
     CDS             complement(300..1451)
                     /locus_tag="VD791_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXV08639.1"
                     /translation="MQAFQFDAPRLPPEALQARRRVREFLAAERAAGRYALHRSSWST
                     YDAEFSRRAGAAGFIGVTWPKEYGGQAQSSLVRFVITEEMLAAGAPCYAHWVADRQSG
                     PQILRNGSERARREILPRICRGECFFGIGMSEPDSGSDLAAVRTRAVRAGGGWRIDGS
                     KIWTSNAHRVHYLIALVRTGEAGEDRHGGLTQFVIDMAQPGVTVRPIHNLSGGHDFNE
                     VHFRDYFVPDDMMVGEAGQGWALVTQELAFERSSPDRFLSDYRLLVELVERAGGIPDA
                     RQEVEIGRLVAHLAALRRMSGAIAGLLDRGISPNVEAALVKDVGTTFEREIPEVARRL
                     FPVQPSLDAHDDVFAETLGHFLLYAPAYTLRGGTREILRGIIARGLGLR"
     gene            1568..2233
                     /locus_tag="VD791_11460"
     CDS             1568..2233
                     /locus_tag="VD791_11460"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXV08640.1"
                     /translation="MQGERLDNIRSIGASTARSAAKEELPPLPARPTLIDILTLRLHK
                     PKFGLPFAAAPHCVQCATLARKAGVDEETVLACLLHDLGMALIRPDHGWWGAQLVEPY
                     VSERVSWAIRYHQALRYYPDASVGYEYPELYVQMFGKDYRPPAYIEAAYREARAHRWY
                     MVARHITLFDDYGFEAQAPFSLEPFADLIGRHFRQPAEGLGNDNSPTAHMWRTIIDPN
                     KPL"
     gene            2241..>2521
                     /locus_tag="VD791_11465"
     CDS             2241..>2521
                     /locus_tag="VD791_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HXV08641.1"
                     /translation="MTQRTIPPFRADHVGSFLRPPALLEARARYKQAAISKEVLRAAE
                     DSAIREAVRLQEDLGLQGITDGEFRRTYFHVDFLERLAGVEVKGGIAAS"
BASE COUNT          382 a          909 c          869 g          361 t
ORIGIN      
        1 gaccatggtt tcgggcagcg gcagcggcac ggtgtgatag ccggcacggc gcatcaggcg
       61 caatccgtcg gccgctgcga gcccgacgcc gccctgcgct tccggcacca gcgcgagcgt
      121 cagccccgca ttcagcaccg cgtcccagat cgcgtgcggc cactcgccgc ggtcggcggc
      181 ggccaggcgc tccgccgtca cctctccggc gaagatgcga tcggcctgct ccagcacgat
      241 gccggacagc tcgtccggct gcggcgccag gccgtcgcgg gtggactgca cggcgcccat
      301 cagcgcaacc ccagcccgcg cgcgatgatg ccgcgcagga tctcgcgcgt gccgccgcgc
      361 aacgtgtagg cgggcgcgta cagcaggaaa tgtcccagcg tctcggcgaa cacgtcgtcg
      421 tgcgcatcca ggctgggctg caccgggaac aggcggcgcg ccacctcggg gatctcgcgc
      481 tcgaaggtcg tgcccacgtc cttgaccagc gccgcctcga cgttggggct gatgccgcgg
      541 tcgagcaggc cggcgatggc gccggacatg cggcgtagcg cagccaggtg cgcgaccagg
      601 cgcccgatct ctacctcctg ccgcgcatcg ggtatgcccc cggcgcgctc gaccagttcc
      661 accagcaggc ggtagtccga cagaaaacgg tccggcgacg aacgctcgaa ggccagctcc
      721 tgcgtcacca gcgcccagcc ctgccctgcc tcgccgacca tcatgtcgtc gggcacgaag
      781 tagtcgcgaa agtggacctc gttgaaatcg tgcccgccgg aaagattgtg gatcggccgc
      841 accgtcacgc cgggctgcgc catgtcgatg acgaactggg tgagtccccc gtggcgatcc
      901 tcgcccgcct cgccggtgcg caccagcgcg atcaagtagt gcacgcggtg cgcgttcgac
      961 gtccagatct tgctgccgtc gatgcgccag ccgccgccgg cgcgcaccgc gcgcgtgcgc
     1021 accgccgcca ggtcggagcc ggaatccggc tcgctcatgc cgatgccgaa gaagcactcg
     1081 ccgcggcaga tgcgcggcag gatctcgcgc cgcgcgcgct cgctgccgtt gcgcaggatc
     1141 tgcgggcccg attgccggtc ggcgacccag tgcgcgtagc acggcgcgcc cgcggccagc
     1201 atctcctcgg tgatgacgaa acgcaccagg gaggactgcg cctgtccgcc gtactccttg
     1261 ggccaggtga cgccgatgaa gccggcggcg ccggcgcggc ggctgaactc cgcatcgtag
     1321 gtgctccacg agctgcggtg cagcgcatag cggccggcgg cccgctccgc ggcgaggaac
     1381 tcccgcaccc gccggcgcgc ctgcagcgcc tcgggcggca gccgcggcgc gtcgaactgg
     1441 aaggcttgca tcgggcaaat atagcaggcc gagagcgctt gcagttttcg cggccccgca
     1501 cagcggctgc cggcatcgag cgccgcggtc ctttgactcg cgccgcgggc ggtgcgatag
     1561 tttaggcatg cagggcgaac gtctcgacaa catcaggagc atcggcgcct cgacggcgcg
     1621 ctccgcggcc aaggaagagc tgccgccgtt gccggcgcgg ccgacgctga tcgacattct
     1681 taccttgcgg ctgcacaagc cgaagttcgg gctgccattt gccgcggcgc cccactgcgt
     1741 gcagtgtgca acgctcgcgc gcaaggccgg ggtggacgag gagacggtgc tcgcctgcct
     1801 gctgcacgac ctgggcatgg cattgatccg gcccgaccac ggctggtggg gcgcgcagct
     1861 ggtcgagccc tacgtctccg agcgggtgtc gtgggcgatc cgctatcacc aggcgctgcg
     1921 ctactacccg gatgcgtcgg tgggctacga gtacccggag ctgtacgtgc agatgttcgg
     1981 caaggactac cggccgcccg cgtacatcga ggccgcttac cgcgaagcgc gcgcgcaccg
     2041 ctggtacatg gtggcgcggc acatcacgct gttcgacgac tacggcttcg aagcgcaggc
     2101 gccgttctcg ctggagccgt tcgccgatct gatcggccgg cacttccgcc agccggcgga
     2161 gggcctgggc aacgacaaca gtccgaccgc tcacatgtgg cgtaccatca tcgatccgaa
     2221 caagccgttg tgactctgtc atgacccaac gcaccatccc gccgttccgc gccgatcacg
     2281 tcggcagctt cctgcgcccg cccgcgctgc tggaggcacg cgcgcgctac aagcaggccg
     2341 cgatttcgaa ggaggtgctg cgcgccgcgg aggacagcgc catccgcgaa gcggtgcgcc
     2401 tgcaggaaga cctcgggctg cagggcatca ccgacggcga gttccgccgc acctacttcc
     2461 acgtcgattt cctggagcgg ctcgccggcg tcgaggtgaa gggcggcatc gcagcgagct
     2521 t
//
LOCUS       DASPGE010000355         8380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_327524, whole genome shotgun sequence.
ACCESSION   DASPGE010000355 DASPGE010000000
VERSION     DASPGE010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8380
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327524"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..484
                     /locus_tag="VD791_11470"
     CDS             185..484
                     /locus_tag="VD791_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08642.1"
                     /translation="MNHSLKPALGLCVLCLALAAPMARAEGDPAPTIERERAMQRYKD
                     CRFTDEMGQYLYQCVKKNDGFGTHWCYDETLETYCPAQAAARKAAESAAEQKPSN"
     gene            743..1228
                     /gene="rimI"
                     /locus_tag="VD791_11475"
     CDS             743..1228
                     /gene="rimI"
                     /locus_tag="VD791_11475"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HXV08643.1"
                     /translation="MSPRAAVLRLATPADANDIAAMSRDLIEHGLGWSWTRGRVAKNI
                     AHRETATLVACSGRRMIGFAMMYFGDDHAHLNLLAVRPAYQRIGVGRRMVAWLVESAK
                     VAGISAIHLEVRSNNTGARRFYRALGFQEVALLPRYYSGVESAIWMARDLRARTTSAR
                     L"
     gene            complement(1363..2484)
                     /locus_tag="VD791_11480"
     CDS             complement(1363..2484)
                     /locus_tag="VD791_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV08644.1"
                     /translation="MRSRILSSLADVAPAEWDALAGASPFLRHAFLHGLEQTGCVAPD
                     TGWTPRHVTLWEGGRLRGAMPLYLKSHSYGEYVFDWGWAEAYQRHGVRYYPKLLCAVP
                     FTPATGPRLLAPSSELRRRLLDAAFTLAAEEHASSLHVLLPPDAQARELEARGLMLRA
                     SVQFHWENPGYRDFDDFLAGMSHDKRKKIKQERRRVREAGIVFRRLSGSEAAEADWDF
                     FTRCYNRTYRQHHSTPYLNRAFFRHLAHSMPEHLVLVLGERDGRPVASALNVLGGATL
                     YGRYWGATEYHSGLHFETCYYQAIEFCIERGVKLFEGGAQGEHKLARGLLPVRTVSAH
                     WLAHPQFAHAVERFLDREAQGIVHYLDELNEHTPFKSTH"
     gene            2622..2960
                     /locus_tag="VD791_11485"
     CDS             2622..2960
                     /locus_tag="VD791_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXV08645.1"
                     /translation="MKKIEAIVKPFKLDEVREALSEIGVTGLTVTDVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEVVVADKLLETAIEAIVRSARTGKIGDGKIFVTTVEQVIRI
                     RTGETDEAAV"
     gene            complement(2978..3550)
                     /locus_tag="VD791_11490"
     CDS             complement(2978..3550)
                     /locus_tag="VD791_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HXV08646.1"
                     /translation="MAKQRLRTNDDGEAFREAVKDVTPLRESGRVVHAPEKPPPLPLQ
                     RLEDNRQVLQDSLSESAEPELESGDALAFLRPGMSPQVLRRLRSGFWAVQEELDLHGA
                     RAHEARALLADFFAAAQSRGLRCVRVIHGKGRGSRRGEPVLKREVAAWLTQRGDVLAY
                     CEARPAEGGAGAVLVLLQSRAAKREVREDE"
     gene            complement(3595..4557)
                     /gene="trxB"
                     /locus_tag="VD791_11495"
     CDS             complement(3595..4557)
                     /gene="trxB"
                     /locus_tag="VD791_11495"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HXV08647.1"
                     /translation="MMATSTKHARLLILGSGPAGYSAAVYAARANLHPVLITGIAQGG
                     QLMTTTDVDNWPADWQGVQGPELMERFQKHAERFGTEIVFDHINTAQLGGKPLTVLGD
                     QAAYTCDALIIATGASAKYLGLPSEQQFMGKGVSACATCDGFFYKGQDVAVVGGGNTA
                     VEEALYLANIARSVTLVHRRDKLRAEKILIDRLMQKAQAGNVRTEWNHTLDEVLGDKS
                     GVTGVRIKHAQSGATRNLEVKGLFIAIGHTPNTQIFDGQLAMENGYIVTAGGREGNAT
                     ATSVPGVFAAGDVQDHVYRQAVTSAGSGCMAALDAEKYLDALGG"
     gene            4784..7081
                     /locus_tag="VD791_11500"
     CDS             4784..7081
                     /locus_tag="VD791_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011852040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HXV08648.1"
                     /translation="MRLDHSFARSRSAVSRQREPLPPRVAGLVRESWWWLLVAAAVFL
                     VLVLSTYDKADPAWSHGVASGEIHNAGGRFGAWVADILLSLFGLSAYWTAVFLVFGTW
                     WGYRRLDSGGSVDRRPFWIASIGFLALLLASASLEALRLYSLPVELPLGPGGILGVSL
                     SAVLHRFFGFTGSTLALLCVGAVGFSLLSGLSWLDLAERIGEWLERGYGALRARWDAA
                     RDRKVGEVATQLREEAVEEEKRILEVHRPLHIEPPQLTTPKSERVARERQKPLFEDLP
                     DSPLPPLALLDETTSSVEALSAETLEYTSRLIEKKLLDFGIAVKVVAAYPGPVITRYE
                     IEPAVGVKGSQVVNLVKDLARALSVVSIRVVETIPGKTCMGLEIPNPRRQVVRLSDIL
                     GSQVYADVVSPLALALGKDIAGKAVVADLGRMPHLLVAGTTGSGKSVAINAMILSLLY
                     KATPNAVRLIMVDPKMLELSAYEGIPHLLAPVVTDMKQAAHALGWCVGEMERRYKLMS
                     ALGVRNLSGFNQKVLEAEKAGHPLRDPLAPSPESPPLKELPLVVVVIDELADLMMVVG
                     KKVEELIARLAQKARAAGVHLILATQRPSVDVITGLIKANIPARVAFQVSAKVDSRTI
                     LDQMGAESLLGQGDMLYLPPGTGHPQRVHGAFVADAEVHKVVQHLKSRASPQYIDGIL
                     EGADEEGAESDAPEESDAEADPLYDQAVEIVLRTRRPSISLVQRHLRIGYNRAARLIE
                     AMERAGLVSSMQTNGNREVLVASKE"
     gene            7252..7869
                     /gene="lolA"
                     /locus_tag="VD791_11505"
     CDS             7252..7869
                     /gene="lolA"
                     /locus_tag="VD791_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HXV08649.1"
                     /translation="MTKFLMLLPALAVWALPVNAGSIEKLRAFAAQTQSARADFSQKM
                     LGKDGKVEQTSTGKLVFSRPGKFRWEYRKPYEQLIIGDGEKLWVYDKDLNQVTVKKLR
                     GALGSSPAELLAGSNEIEDHYNLNAMGVKGGLDWLDAYPTDGDSMFEKVRLGFKGNGL
                     DIMELHDRLGQVTVIRFTKVQRNPKLDATAFRFTPPEGADVLQDE"
     gene            7890..>8380
                     /locus_tag="VD791_11510"
     CDS             7890..>8380
                     /locus_tag="VD791_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXV08650.1"
                     /translation="MRPQRLEDVVGQRHLLAPGKPLEVAARAGKLHSMILWGPPGVGK
                     TTLARLLARAGNAEFIALSAVFSGVKDIREAVAQAERTLREAGRHTILFVDEVHRFNK
                     SQQDAFLPFVEQGLFTFIGATTENPSFEVNSALLSRASVYVLKPLGEDELGVLFDRAC
                     SGPL"
BASE COUNT         1283 a         2842 c         2937 g         1318 t
ORIGIN      
        1 cctacgagca gggcggacaa gatccacagc aggatttcca agcgatgtct ccccgttggt
       61 cagaggtcgg tcagggtcgg tcgggtcggt cgggcgcgtc tgtcgccgcc ggtgccattc
      121 acgtagaata tgttgctccg catcgacttg gtggttgatc gaccatcacc caggaaggac
      181 gttcatgaat cattcgctca agccggccct cggcctttgc gtgttgtgcc tggccctggc
      241 cgcgcccatg gcgcgtgccg agggcgatcc ggcgcccacc atcgagcgcg agcgcgccat
      301 gcagcgttac aaggactgcc gattcaccga cgagatgggg cagtacctgt accagtgcgt
      361 caagaaaaac gacggcttcg gcacccactg gtgctacgac gagaccctgg agacgtattg
      421 cccggcccag gccgccgcgc gcaaggccgc cgagagcgcg gccgagcaga agccctccaa
      481 ctgaagcgcg gtttcggctc ggcgcgaaag gccgcgccgc ccgcggcctt tctgtttggc
      541 gccggcgcag gacacgctat actgctgatc gctaccccgc acgccggggg gtgaaagcat
      601 gtctgacttt cggcgcatcc gggtagcgga tgcgcagacc taggtcaatt ccggctaggt
      661 caattctggg taggttcggg gtgaattcgg caagcgcgtc acgccgcggg ccccgcggca
      721 atcccggtcg aggtcgccgg ccatgagccc gcgcgcggcc gtgctgcggc tggccacgcc
      781 ggcggacgcc aacgacatcg ccgcgatgtc gcgcgatctg atcgagcacg gcctgggctg
      841 gtcgtggacg cgcggccgcg tggcgaagaa catcgcccat cgcgagaccg ccaccctggt
      901 ggcctgcagc gggcggcgca tgatcggctt cgccatgatg tatttcggcg acgatcacgc
      961 gcacctcaac ctgctcgccg tgcgcccggc gtatcagcgc atcggcgtgg ggcggcgcat
     1021 ggtcgcctgg ctggtggagt cggcaaaggt cgccggcatc tccgccatcc acctcgaggt
     1081 gcgcagcaac aacaccggcg cgcgccgctt ctatcgcgcg ctcggcttcc aggaggtagc
     1141 gctgctgccg cgctactaca gcggcgtgga gtcggcgatc tggatggcgc gcgacctgcg
     1201 cgcgcgcacc accagcgcgc ggctgtagaa ggtctcccct cctgccaagg aggggtgcct
     1261 cgactcccct cctggcaagg aggggtggcg ccggaggcga cggggtggtg gcgggcgggg
     1321 tggttcaact gcgcctgctg accgaacccg cttgacagaa ggtcaatgtg tcgacttgaa
     1381 cggcgtgtgc tcgttcaact cgtccaggta gtgcacgatc ccctgcgctt cgcggtcgag
     1441 gaagcgctcc accgcgtgcg cgaactgcgg atgcgccagc cagtgcgccg acaccgtgcg
     1501 caccggcagc aggccgcgcg cgagcttgtg ctcgccctgg gccccgccct cgaacagctt
     1561 cacgccgcgc tcgatgcaga actcgatcgc ctggtaataa caggtctcga agtgcaggcc
     1621 cgagtggtac tcggtggcgc cccagtagcg gccgtagagc gtggcgccgc cgagcacgtt
     1681 cagcgcgctc gccaccggcc gcccgtcgcg ctctccgagc accagcacca ggtgctcggg
     1741 catgctgtgc gcgaggtggc ggaagaaggc acggttgaga tacggcgtgg agtggtgctg
     1801 gcggtaggtg cggttgtagc agcgcgtgaa gaaatcccag tcggcctcgg ccgcctcgct
     1861 ccccgacagg cgccggaaca cgatcccggc ctcgcgcacg cggcgccgct cctgcttgat
     1921 cttcttgcgt ttgtcatggc tcataccggc caggaagtcg tcgaagtcgc ggtagccggg
     1981 gttttcccag tggaactgca cgctggcgcg cagcatcagg ccgcgcgctt ccagctcgcg
     2041 agcctgcgcg tccggcggca gcagcacgtg cagcgacgac gcatgctcct ccgcggccag
     2101 cgtgaaggcg gcgtccagca gccggcgccg cagctcggac gagggcgcca gcagacgcgg
     2161 cccggtggcg ggggtgaacg gcaccgcgca cagcagcttg gggtagtagc gcaccccgtg
     2221 gcgctggtag gcctcggccc agccccagtc gaacacgtac tcgccgtagg aatggctctt
     2281 gagatagagc ggcatcgcgc cccgcagccg gccgccttcc caaagcgtga cgtggcgggg
     2341 cgtccagccg gtgtcgggcg cgacgcagcc ggtttgctcg agcccgtgca ggaaggcgtg
     2401 ccgcaggaac gggctggcgc cggccagcgc gtcccattcg gccggcgcga catcggccag
     2461 gctggagagg atgcgcgatc tcatcgggga gtcccgtgcg ccgggagcgg cgggggcggg
     2521 cagattctat tgcagcgcgg gagtgactat actgtagccg ggcccgcccg ggcgccgttc
     2581 atcactgcac cgttcatcac tcacttcaag tggggagcga aatgaagaag atcgaagcca
     2641 tcgtgaagcc gttcaagctg gacgaggtac gcgaggcgct gtccgagatc ggcgtcaccg
     2701 gcttgacggt gaccgacgtg aagggcttcg ggcgccagaa gggtcatacc gagctgtatc
     2761 gcggcgccga gtacgtggtg gatttcctgc ccaaggtcaa ggtcgaggtg gtggtcgcgg
     2821 acaagctcct ggaaaccgcg atcgaggcca tcgtgcgctc ggcgcgtacc ggcaagatcg
     2881 gcgacggcaa gatcttcgtc accaccgtcg agcaggtgat ccgcatccgc accggcgaga
     2941 ccgacgaagc ggcggtctga gcaccccggc ggggcgctca ctcgtcctcg cgcacctcgc
     3001 gtttcgcggc gcgcgactgc agcagcacca gcaccgcgcc ggcgccgcct tccgccgggc
     3061 gcgcctcgca gtacgccagg acgtccccgc gctgggtcag ccaggccgcg acctcgcgct
     3121 tgagcaccgg ctcgccacgc cgcgagccgc gccccttgcc gtggatcacg cgcacgcagc
     3181 gcagcccgcg gctctgcgcc gcggcgaaga agtcggcgag cagcgcacgc gcctcgtgcg
     3241 cacgcgcgcc gtgcagatcc agctcttcct gcaccgccca gaacccgctg cgcagccggc
     3301 gcagcacctg cggcgacatg cccggccgca ggaacgccag cgcgtcgccc gactccagct
     3361 ccggctcggc ggattcgctc aggctgtcct gcagcacctg ccggttgtcc tcgagccgtt
     3421 gcagcggcag cggcggcggc ttctccggag cgtgcaccac ccggcccgat tcgcgcagcg
     3481 gcgtgacgtc cttcaccgcc tcgcggaacg cttccccgtc gtcgttcgtg cgcaatcgct
     3541 gtttggccat tgtgatcacg cccttgccca ggcgcgtccg cgcgcggcga tgacctaccc
     3601 gccgagcgcg tcgagatact tctccgcgtc gagcgccgcc atgcagccgc tgccggcgct
     3661 ggtcaccgcc tggcggtaca cgtgatcctg cacgtcgccc gcggcgaaca cgccggggac
     3721 gctggtggcg gtggcgttgc cctcgcgccc gcccgcggtg acgatgtagc cgttttccat
     3781 cgccagctgc ccgtcgaaga tctgcgtgtt cggcgtgtgg ccgatggcga tgaacaatcc
     3841 cttcacctcg aggttgcggg tcgcgccgct ctgggcgtgc ttgatgcgca cgccggtgac
     3901 gccgctcttg tcgcccagca cctcgtcgag cgtgtggttc cactcggtgc gcacgttgcc
     3961 cgcctgcgcc ttctgcatca gccggtcgat caggatcttc tcggcgcgca gcttgtcgcg
     4021 ccggtgcacc agggtgacgc tgcgtgcgat gttggccaga tacagcgcct cttccaccgc
     4081 cgtgttgccg ccgccgacca cggccacgtc ctgtcccttg tagaagaacc cgtcgcaggt
     4141 cgcgcacgcc gacacgccct tgcccatgaa ctgctgctcc gagggcagcc ccaggtactt
     4201 ggccgacgcc ccggtggcga tgatcagcgc atcgcaggta taggcggcct ggtcgccgag
     4261 cacggtgagc ggcttgccgc cgagctgcgc ggtgttgatg tggtcgaaga cgatctcggt
     4321 gccgaagcgc tcggcgtgct tctggaagcg ctccatcagc tcggggccct ggacgccctg
     4381 ccaatcggcc ggccagttgt ccacgtcggt ggtggtcatc agctgtcccc cctgggcgat
     4441 gccggtgatc agcacggggt gcagattggc gcgggcggcg tacaccgcgg cgctgtagcc
     4501 ggcggggccc gagcccagga tcaggagacg tgcgtgcttg gtcgaggtgg ccatgatgcg
     4561 cggtagatgg gggcgaaggt ccgcgaaacc aggaatctag cagaccggga atctactagc
     4621 agacggggaa tctaacagtc cgggcgagcc ggtgtcgagc cgccgcaccg ggatgccgaa
     4681 ccgccgcacc gggatgccgg acccgggcgt ggctataatg cggcatggca ggcaatcgtt
     4741 ccgcgcggga caccgtcggg gaagaatcgc tcggtcgctc gacgtgagac tcgatcacag
     4801 cttcgcgagg tccaggtccg cagtctccag gcagcgcgag ccgctgccgc cccgggtcgc
     4861 cggcctggtg cgcgaatcct ggtggtggct gctggtggcc gccgccgtct tcctggtgct
     4921 cgtcctgtcc acttacgaca aggccgaccc ggcctggtcg cacggcgtcg cgtcgggaga
     4981 gatccacaac gcaggcggac gcttcggcgc ctgggtggcc gacatcctgc tgtccctgtt
     5041 cggcctgtcc gcgtactgga ccgcggtgtt tctggtgttc ggcacctggt ggggttaccg
     5101 ccggctcgac agcggcggca gcgtcgaccg caggccgttc tggatcgcct cgatcggatt
     5161 cctcgcgctg ctgctggcga gcgccagcct cgaggcgctg cgcctgtact cgctgccggt
     5221 cgagctgccg cttggccccg ggggcatcct cggcgtgagc ctgagcgcag tgctgcacag
     5281 gtttttcggt ttcaccggca gcaccctggc gctgctgtgc gtgggcgcgg tcggcttcag
     5341 cctgttgtcc gggctgtcct ggctggacct ggcggagcgc atcggcgagt ggctcgagcg
     5401 cggctacggg gcgttgcgcg cgcgctggga cgccgcgcgc gaccgcaagg tcggcgaggt
     5461 cgccacacag ctgcgcgaag aggcggtgga ggaggagaag aggatcctcg aggtgcaccg
     5521 cccgctgcac atcgagccgc cgcagctcac cacgccgaag tccgagcgcg tcgcccgcga
     5581 gcgccagaag ccgctgttcg aggacctgcc ggactcgccg ctgccgccgc tggcgctgct
     5641 cgacgagacc acctccagcg tcgaggcgct gtcggccgag acgctcgagt acacctcgcg
     5701 cctgatcgag aagaagctgc tcgacttcgg catcgcggtg aaggtggtgg ccgcctatcc
     5761 cgggccggtg atcacgcgct acgagatcga gcccgcggtc ggcgtcaagg gcagccaggt
     5821 ggtgaacctg gtcaaggacc tggcgcgcgc gctgtcggtg gtgagcatcc gcgtggtgga
     5881 gaccatcccg ggcaagacct gcatggggct ggagatcccc aatcccaggc gccaagtggt
     5941 gcgcctgtcc gacatcctcg gctcgcaggt gtacgccgac gttgtctcgc cgctcgcttt
     6001 ggccctgggc aaggacatcg ccggcaaggc ggtggtcgcg gacctggggc gcatgccgca
     6061 cctgctggtg gccggcacta ccggttccgg caagtcggtg gcgatcaacg ccatgatcct
     6121 gagcctgctg tacaaggcca cgcccaacgc ggtgcgcctg atcatggtag acccgaagat
     6181 gctggagctg tccgcctacg agggcattcc gcacctgctg gcgccggtgg tcaccgacat
     6241 gaagcaggcc gcgcacgcgc tcggctggtg cgtgggcgag atggagcggc gctacaagct
     6301 gatgtccgcg ctgggcgtgc gcaatctttc cggcttcaat cagaaggtgc tcgaggccga
     6361 gaaggccggc cacccgctgc gcgacccgct tgccccgagt ccggagtcgc ctccgctcaa
     6421 ggagctgcct ctggtggtgg tggtgatcga cgagctggcc gacctgatga tggtggtggg
     6481 caagaaggtc gaggagttga tcgcgcgcct ggcgcagaag gcgcgggccg cgggcgtgca
     6541 cctgatcctg gccacccagc gcccgtcggt ggacgtgatc accggcctga tcaaggccaa
     6601 catccccgcg cgcgtggcgt tccaggtgtc ggccaaggtc gattcgcgca ccattctcga
     6661 tcagatgggc gccgagtcgc tgctcggcca gggcgacatg ctctacttgc cgcccggtac
     6721 cgggcacccg cagcgcgtgc acggcgcgtt cgtggccgac gccgaagtgc acaaggtggt
     6781 ccagcacctg aagtcgcgcg cctcgccgca gtacatcgac ggcatcctgg agggcgcgga
     6841 cgaggagggc gccgagtccg acgcgccgga ggagagcgac gccgaggccg acccgctgta
     6901 cgaccaggcc gtggagatcg tgctcaggac gcggcgcccg tcgatctcgc tggtgcagcg
     6961 gcacctgcgc atcggctaca accgcgccgc ccggctgatc gaagccatgg agcgggccgg
     7021 gctggtgtcc agcatgcaga ccaacggcaa ccgcgaggtg ctggtggcgt ccaaggagtg
     7081 atgccgagag gcgtgaccgg cactgcgtga ccgaagggcg tgaccgacga gcgtgacaac
     7141 cccgcgccca ggccacgcga gccgtcgtgc ctagcgcgtt cccagtcacg cttttcggtc
     7201 actcagtacc ggtcacgctt ttcggtcacg cctttccaca acgagggcta catgacgaag
     7261 ttcctgatgc tgctgccagc gctcgccgtc tgggcgcttc ccgtaaacgc cggctccatc
     7321 gagaagctgc gcgctttcgc cgcgcagacg cagtccgcgc gcgccgattt cagccagaag
     7381 atgctcggca aggacggcaa ggtggagcag acctcgaccg ggaaactggt cttctcgcgg
     7441 cccggcaagt tccgctggga gtatcgcaag ccctacgagc agctcatcat cggcgacggc
     7501 gagaagctgt gggtctacga caaggacctc aaccaggtca ccgtgaagaa gctgcgcggc
     7561 gcgctcggca gcagcccggc ggagctgctc gcggggagca acgagatcga agaccactac
     7621 aacctcaacg ccatgggcgt caagggcggc ctcgactggc tggacgccta tcccacggac
     7681 ggcgacagca tgttcgagaa ggtgcgcctg ggcttcaagg gcaacggcct ggacatcatg
     7741 gagctgcacg atcgcctggg gcaggtcacc gtcatccgct tcaccaaggt ccagcgcaac
     7801 ccgaagctcg acgcaaccgc attccgcttc accccgcccg agggggcgga cgtgctgcag
     7861 gacgagtgac cgcccctctc gccgaacgca tgcgcccgca gcgtctcgag gacgttgtgg
     7921 ggcagcggca cctgctggcg ccgggcaagc cgctggaggt ggctgcgcgc gccggcaagc
     7981 tgcactcgat gatcctgtgg gggccgccgg gcgtgggcaa gaccaccctg gcgcggctgc
     8041 tggcgcgagc gggcaacgcg gaattcatcg cgctgtccgc ggtgttctcc ggcgtaaagg
     8101 acatccgaga ggcggtggcg caggccgagc gtaccctgcg cgaggccggc cgccacacca
     8161 tcctgttcgt ggacgaggtg caccgcttca acaagtcgca gcaggatgct ttcctgccgt
     8221 tcgtggagca gggactgttc accttcatcg gcgccaccac cgagaatccg tccttcgagg
     8281 tgaacagcgc gctgctgtcg cgggcttcgg tatacgtgct caagcccctc ggcgaggacg
     8341 agctgggcgt tctgttcgat cgtgcttgca gcggaccgct
//
LOCUS       DASPGE010000356         6765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_327689, whole genome shotgun sequence.
ACCESSION   DASPGE010000356 DASPGE010000000
VERSION     DASPGE010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327689"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VD791_11515"
     CDS             <1..1194
                     /locus_tag="VD791_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXV08651.1"
                     /translation="ALVLAPTRELAAQVWEAICEYGKHIPLRSACVYGGVAIEPQMKD
                     LRAGVEILVATPGRLLDLVHQRCANLSTVQILVLDEADRMLDMGFLPDIKRILALLPL
                     QRQNLMFSATFPDDIRKLGDQVLQAPVRIQIAPRYAVAELVTHSAYKIDPLRKRELLG
                     DLIRSRDMRQVLVFTSTKISANRLARQLERDGFNCSALHSDKTQQERTATLAAFKEGK
                     LQVLVATDVASRGLDIEQLPHVINYELPPNSEDYIHRIGRTGRAGVPGEAISLVEPEE
                     QDLLAGIERLLKKKIPIQQLPAEGSRPRERTRERGARAAPRPGPKPTQSIPAQGRPSQ
                     VNPSLGRLSQNSPAESTTNGSGQPAPALVSTARSGDAGAAGAPERRRKQVAALFLPPV
                     PAKQD"
     gene            1200..2060
                     /locus_tag="VD791_11520"
     CDS             1200..2060
                     /locus_tag="VD791_11520"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV08652.1"
                     /translation="MAALGAEQAGSVEARFRGLAEGPEERIDLAEGALLIAKHADEDL
                     DVERYLARIDELAARLRARIPQGGTEPERIAALNRFLFAEQGFAPNAEDYYDPRNSFL
                     NQVLDRRVGIPISLSVLYIEVGRRVGLPLRGVAFPGHFLVKCDLGAGVVVLDPYCGGI
                     SLGLQDLQRRLREVRGGEVSRAIVAGMLVAANKKEILARMLRNLKAIYLQQHDYAHAL
                     PTLDWILLLRPGEVAELRDRGLTYHKLECVRAALSDLERYLEKAPAAADRDEIRSRVI
                     ELRRSAARLN"
     gene            2128..3084
                     /locus_tag="VD791_11525"
     CDS             2128..3084
                     /locus_tag="VD791_11525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV08653.1"
                     /translation="MTGLGDFLNRVRKNARHWGRWARRRGIACYRVYDRDIPEFAFAL
                     DVYGERAHLQEYDRAGGHPGDEAAGREQRLRQVRLAAAEGLGFDPQRVVLKRRARRGA
                     GEQHEKTGRQGEDFVVEEGGHRFLVNLEAHLDTGLFLDHRNTRALVAAEAPGKRFLNL
                     FCYTAGFTVYAAAAGAASSVSVDLSNTYLDWARRNFALNRIDTRRHLLERAEVRRWLE
                     EAAAAGRRFDLIVLDAPAFSTSKAMSGVLDIQRDYPALLSGCAGLLGDGGVLYFSTNL
                     RTFKFDAAAAQPLTAEEISRRTVPEDFRNRRMHRCWRLVRKA"
     gene            complement(3102..3623)
                     /locus_tag="VD791_11530"
     CDS             complement(3102..3623)
                     /locus_tag="VD791_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08654.1"
                     /translation="MTTALASFRSLAGAILLAVAPAALAAEPVPPPPPIPAGDTAPAA
                     APAATDVKGKCVNCGVVRSIRAVERERKASREVPAYMTSDQYLNTRRYSEPRVGPVFG
                     LTFGPGQETKSFVGAAGNEAMRQRMLEIVYEIVVLYDDGRFGLIEQPDRGALRVGDRV
                     RVVDDRVELAPRE"
     gene            complement(3646..4494)
                     /locus_tag="VD791_11535"
     CDS             complement(3646..4494)
                     /locus_tag="VD791_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HXV08655.1"
                     /translation="MKRLPFLLLGLLLAAGTHAAEPAGALALIKTTGVIRLGYLDSAP
                     PFAFDRGGTPSGYSVDLCAHVASDIGRQLDAPLRVEWVKLTLQNRLAAVRDGRVHVEC
                     GTTTWTLSRQEQVDFSLMTFIDGGTLLVGAGSAVQRLADLGGKRIGVVPGTTTHGSLR
                     AALEQRRLTAEVVDVASPDQGIELLSAGKLDGYASDRLVLLGLGLSASGAHSFRLLDE
                     DFSVEPYALTLPRGEHDLRLAVNRALARLYRSGEIQSVFDRWLGPLGSPSMLLAALYY
                     LQSIPE"
     gene            complement(4686..5492)
                     /locus_tag="VD791_11540"
     CDS             complement(4686..5492)
                     /locus_tag="VD791_11540"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HXV08656.1"
                     /translation="MHVPARAAYIVPFLAAALAGCATPKNPRDPLEPFNRTIYAFNHD
                     LDRVALKPGALAYREVVPLPVRGGITSFFGNFRDVTAAINSLLQLKVKRAVAGAGRVA
                     INSTVGIFGVFDVATRLGLEKYDEDFGQTLGHWGVKDGPYLVLPLLGPSNARDAVGWV
                     GDFYTDPEFYLFEDNPENWLVFGTRVVNLRANLLEAERLLDEAALDRYAFLREAYLQR
                     RRNLIYDGNPPDAPDGTQAPRRKTLKEMEEELELGEPPVSPAPPGDGSTQ"
     gene            5634..6413
                     /locus_tag="VD791_11545"
     CDS             5634..6413
                     /locus_tag="VD791_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV08657.1"
                     /translation="MDLGIAGKAALVCAASKGLGRGCALGLAREGVNLTLVARTRETL
                     EATAEALRKATGAKVTTVAADITTPQGRAQALAACPQPDILVNNAGGPPAGDFRNWDR
                     EVWIKALDANMLTPIELIKATVDGMIARKFGRIVNITSAAVKAPIPILGLSNGARSGL
                     TGFVAGLARQTVMHNVTINNILPGLFETDTMVSRIDAMAKQSGKTFEQVKQERLKTIP
                     AARLGSPEEFGQLCAYICSAQASFITGQNFLIDGGAYPGTL"
     gene            6476..>6765
                     /locus_tag="VD791_11550"
     CDS             6476..>6765
                     /locus_tag="VD791_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXV08658.1"
                     /translation="MNAPREKFALPLKDEKLFRQQCYVDGQWADADSKQTIVVSNPAT
                     GAAVGTIPRMGVAETRRAVEAADRALPAWRAKTGKERAAILRKWFDLMLANQ"
BASE COUNT         1081 a         2314 c         2387 g          983 t
ORIGIN      
        1 gcgctggtgc tcgcgcccac gcgcgagctg gccgcgcagg tgtgggaagc catctgcgaa
       61 tacggcaagc acatcccgtt gcgctcggcc tgtgtttacg gcggcgtggc catcgagccg
      121 cagatgaagg acctgcgtgc cggggtcgag atcctggtgg ccacgcccgg gcgcctgctc
      181 gatctggtgc accagcgctg cgccaatctg tcgacggtgc agatcctggt gctggacgaa
      241 gccgaccgga tgctcgacat gggcttcctg ccggacatca aacgcatcct ggcgctgctg
      301 ccgctgcagc gccagaacct gatgttctcc gccaccttcc ccgatgacat ccgcaagctc
      361 ggcgaccaag tgctgcaggc gccggtgcgc atccagatcg cgccgcgcta cgccgtggcg
      421 gagctggtga cgcacagcgc ctacaagatc gatccgctgc gcaagcgcga gctgctgggc
      481 gacctgatcc gcagccgcga catgcgccag gtgctggtgt tcaccagcac caagatctcc
      541 gccaaccggc tcgcccgcca gctcgagcgc gacggcttca actgctcggc cctgcacagc
      601 gacaagacgc aacaggagcg caccgcgacg ctggccgcat tcaaggaggg caaactgcag
      661 gtgctggtag ccaccgacgt cgcctcgcgc gggctggata tcgagcagct gccgcacgtg
      721 atcaattacg agctgccgcc caactccgag gactatatcc accgcatcgg ccgcactggc
      781 cgcgcgggcg tgccgggcga ggccatttcg ctggtggagc cggaggagca ggacctgctc
      841 gccgggatcg agcgcctgct gaagaagaag atcccgatcc agcagttgcc cgcggaaggt
      901 tcgcggccgc gcgagcgcac cagggagcgt ggtgcccgtg ccgcgccgcg gcctgggccc
      961 aagccgacgc agagcatccc ggcgcagggc aggccatccc aggtcaatcc atccctgggc
     1021 cgcctatccc agaacagccc ggccgagagc accacgaacg ggagcggcca gcccgctccc
     1081 gcgctggtca gcacggcgcg ttcgggcgac gcgggcgccg ccggagcgcc ggagcggcgc
     1141 cgcaagcagg ttgccgcgct gttcctgcca ccggtccccg ccaagcagga ctgaagcccg
     1201 tggcggccct tggcgccgag caggcaggca gcgtcgaggc gcgcttccgc ggcctggccg
     1261 agggccccga ggagcgcatc gatctcgccg aaggcgcgct gctgatcgcc aagcacgccg
     1321 acgaggatct cgacgtcgaa cgctatctgg cgcgcatcga cgagctggcg gcgcgcctgc
     1381 gcgcgcgcat cccgcagggc ggcaccgagc ccgagcgcat cgccgcgctc aaccgcttcc
     1441 tgttcgccga gcagggcttc gcgcccaacg ccgaggacta ctacgacccg cgcaacagtt
     1501 tcctcaacca ggtcctggat cgcagggtcg gcatcccgat cagtctgtcg gtcctctaca
     1561 tcgaggttgg gcgccgcgtc ggcctgccgc tgcgcggggt ggccttcccc gggcatttcc
     1621 tggtgaagtg cgacctgggt gccggtgtgg tggtgctcga cccctattgc ggcggcatct
     1681 cgctgggact gcaggacctg cagcggcgcc tgcgcgaggt gcgcggcggc gaagtatcgc
     1741 gggcgatcgt cgcgggcatg ctggtagccg ccaacaagaa ggagatcctg gcgcggatgc
     1801 tgcgcaacct caaggcgatc tatctgcagc agcacgatta cgcgcatgcg ctgcccacgc
     1861 tcgactggat cctgctcctg cgcccgggcg aggtcgcgga gctgcgcgac cgcggcttga
     1921 cctaccacaa gctcgagtgc gtccgggccg cgctgagcga tctggagcgc tacctcgaga
     1981 aggcgcctgc ggccgccgat cgggacgaga tccgcagccg cgtcatcgag ctgcggcgca
     2041 gcgccgcgcg gctgaactga acccgccccg gctcgaccgc gcggcgttcg cgctgcctgc
     2101 gtcgagcggg cgctcattgc gcaggccatg accgggctcg gcgatttcct gaaccgggtg
     2161 cgcaagaacg cgcgccactg ggggcgctgg gcgcgccggc gcggcatcgc atgctatcgg
     2221 gtctatgacc gcgacatccc cgagttcgcc ttcgcgctgg acgtgtacgg cgagcgcgcg
     2281 cacctgcaag agtacgaccg cgccggcggg catccgggcg acgaggcggc cgggcgcgaa
     2341 cagcggctgc gccaggtgcg gctggctgcg gcggaggggc tggggttcga tccccagcgc
     2401 gtggtgctca agcgccgcgc gcggcgcggc gccggcgagc agcacgagaa gaccggccgg
     2461 cagggcgagg atttcgtggt ggaggagggc gggcaccgct tcctggtgaa cctggaagcg
     2521 catctggaca ccggcctgtt tctcgatcat cgcaacacgc gcgctctggt cgcggccgag
     2581 gcgcccggca agcgcttcct caatctgttc tgctacacgg ccggcttcac ggtgtacgcg
     2641 gccgccgccg gcgcggcctc ctcggtgagc gtggacctgt cgaacaccta tctcgactgg
     2701 gcgcggcgca acttcgcgct gaatcgcatc gacacccggc gccatctgct cgagcgcgcc
     2761 gaggtgcggc gctggctgga ggaggcggcg gccgcgggac gtcgcttcga tctgatcgtg
     2821 ctcgacgcgc ccgcgttctc gacctcgaaa gcgatgagcg gcgtgctcga catccagcgc
     2881 gactacccgg cgctgctctc cggctgcgcg ggtctgctgg gcgatggcgg ggtgctgtac
     2941 ttctcgacca acctgcgcac cttcaagttc gatgcggcgg ccgcgcagcc gctgaccgca
     3001 gaggagatat cccggcgtac cgtgcccgag gatttccgca accggcgcat gcaccggtgc
     3061 tggcggttgg tacgaaaagc gtgaccggtc acgcctttcg gtcactcccg cggcgccagc
     3121 tccacgcggt cgtccaccac gcgcacccgg tcgcccacgc gcagcgcgcc gcgatcgggc
     3181 tgctcgatca ggccgaagcg gccgtcgtcg tagagcacca cgatctcgta gacgatctcc
     3241 agcatgcgct ggcgcatggc ctcgttgccc gcggcgccga cgaagctctt ggtttcctgg
     3301 cccgggccga aggtcagtcc aaacaccgga cccacgcgcg gctcggagta gcgccgcgtg
     3361 ttgaggtatt gatcggaggt catgtaggcg ggcacctcgc gcgaggcctt gcgctcgcgc
     3421 tccaccgccc ggatggagcg cacgacgccg cagttcacgc atttgccctt gacgtccgtg
     3481 gcagccggcg cggccgccgg cgcggtgtcg ccggcaggta tcggcggggg aggcgggacg
     3541 ggttccgcgg cgagcgccgc aggcgcaacg gcgagcagga tcgcaccggc gagcgatcga
     3601 aacgaggcca acgccgtggt cacggtgcgg tgcctcggtg ctgccctact cggggatgct
     3661 ttgcaggtaa tacagggccg caagcagcat gctgggcgaa cccagcgggc cgagccagcg
     3721 atcgaatacc gactgaatct cgccgctgcg atacagccgc gcgagcgcgc ggttcaccgc
     3781 cagccgcaga tcgtgctccc cgcgcggcag cgtcagggcg tagggttcca cggagaaatc
     3841 ctcgtccagg agccggaagc tgtgcgcgcc gctggcgctc agccccaggc ccagcagcac
     3901 caggcggtcg gatgcgtagc cgtcgagctt gcccgcggac agcagttcga tgccttggtc
     3961 cggggaggcc acgtccacca cctcggcggt gaggcggcgc tgttcgaggg ccgcgcgcag
     4021 gctcccatgc gtagtggttc ccggcaccac gccgatgcgc ttgccgccca ggtcggccag
     4081 gcgctgcacc gcgctgcccg cgcccaccag cagcgtgccg ccgtcgatga acgtcatcag
     4141 gctgaagtcc acctgctcct ggcgcgacag cgtccaggtg gtggtgccgc attcgacgtg
     4201 cacgcgtcca tcgcgcacgg ccgccagccg gttctgcagc gtgagcttga cccactctac
     4261 gcgcagcggc gcgtcgagtt ggcggccgat gtcgctcgcc acgtgtgcgc acaggtccac
     4321 ggaatagccg ctcggggtgc cgccgcgatc gaaggcgaac ggcggcgcgg aatcgagata
     4381 gccgaggcgg atcacgcccg tggtcttgat cagggccagt gcgccggcgg gctcggccgc
     4441 atgggtgccg gccgcgagca gcaggccgag cagcaggaag gggagtcgtt tcatcggatg
     4501 cgcaggtcgg ctatcaggat gacgaggcca cagtatagcg ggggcggatg gtgactgcgt
     4561 gaccgagagg cgtgaccgga gagcgcatcc gggaggcgtg accgggaagc gtgaccgagg
     4621 ggcgtgaccg ggaagcgtga ccggaagaca tgactgccga ttggtaccgc atgagctctc
     4681 gcgctttact gcgtcgagcc gtcgcccggt ggcgcgggcg agaccggcgg ctcgcccagc
     4741 tccagttcct cctccatctc cttcagcgtc ttgcgccgcg gcgcttgcgt gccgtccgga
     4801 gcatccggag gattgccgtc gtagatcaag ttgcggcggc gctgcaagta cgcctcgcgc
     4861 aggaacgcgt agcggtccag cgccgcctcg tcgagcaggc gctcggcctc gagcaggttg
     4921 gcgcgcaggt tgaccacgcg ggtgccgaac acgagccagt tctccgggtt gtcttcgaac
     4981 agatagaact ccggatcggt gtagaagtcg cccacccagc cgaccgcatc gcgggcgttg
     5041 ctcggcccca gcagaggcag caccaggtag ggtccgtcct tgacgcccca gtgccccagc
     5101 gtctggccga agtcctcgtc gtacttctcc agcccgagcc gcgtcgccac gtcgaacacc
     5161 ccgaagatgc ccaccgtgct gttgatcgcc acccgccccg cgccggccac ggcgcgcttg
     5221 actttcaact gcagcaggct gttgatcgcg gcggtgacgt cgcggaaatt gccgaaaaag
     5281 ctggtgatgc cgccgcgcac cgggagcggc accacttcgc gataggccag cgcgccgggc
     5341 ttcagcgcga cgcgatccag gtcgtggttg aaggcgtaga tggtgcgatt gaaaggttcc
     5401 agcggatcgc gcgggttctt cggggtggcg cagccggcga gcgcggcggc gagaaacggg
     5461 acgatgtatg cggcgcgggc aggaacgtgc atcggtgcgg attgttattg ctggaccgga
     5521 ttatagcaac ggcgctcggg cggcgacctt gagcggagcg tcgtttgcaa acgtcgcgcg
     5581 gatgcctcag cataccgggc tttccgctgc aacgcttccg ggaggacatc gcaatggacc
     5641 tgggaatcgc aggcaaggcg gcgctggtgt gcgccgcgag caaggggctc ggcagaggct
     5701 gcgcgctggg gctggcgcga gagggcgtga acctgacgct ggtggcgcgc acgcgcgaga
     5761 cgctcgaggc gacggccgag gcgttgcgca aggcgactgg cgcgaaggtg accacggtgg
     5821 cggcggatat caccacgccg cagggccgcg cgcaggcgct cgccgcctgc ccgcagcccg
     5881 acatcctggt gaacaacgcc ggcgggcccc ccgcgggcga cttccgcaac tgggatcgcg
     5941 aggtgtggat caaggcgctg gacgccaaca tgctcacgcc gatcgaactg atcaaggcca
     6001 ccgtggacgg catgatcgcg cgcaagttcg ggcgcatcgt caacatcacc tcggcggcgg
     6061 tcaaggcgcc gattccgatc ctggggctgt ccaacggggc gcgttccggc ctcaccggct
     6121 tcgtcgccgg gctcgcacgc cagaccgtga tgcacaacgt caccatcaac aatatcctgc
     6181 cgggcctgtt cgagaccgac accatggtga gccgcatcga cgccatggcc aagcagagcg
     6241 gcaagacctt cgagcaggtg aagcaggagc gcctgaagac catcccggcc gcgcgcctcg
     6301 gttcgccgga ggaattcggc cagctgtgcg cgtacatctg cagcgcacag gcgagcttca
     6361 tcaccgggca gaacttcctg atcgacggcg gtgcttaccc gggcacgctg tgattcgttt
     6421 cgccgcgccg aagcctataa ttgttcctac cagacccagc aacgaggtgc accaaatgaa
     6481 cgcgccgcgc gaaaaatttg ccttgccgct caaggacgag aagctgttcc gccagcagtg
     6541 ctacgtggac ggccagtggg ccgacgcgga cagcaagcag accatcgtcg tctccaatcc
     6601 cgccaccggc gcggcggtgg gcaccatccc gcgcatgggc gtggcggaaa cgcggcgcgc
     6661 cgtcgaggcc gcagaccggg cgcttcccgc ctggcgcgcg aagaccggca aggaacgcgc
     6721 cgccatcctg cgcaagtggt tcgatctgat gctggcgaac cagga
//
LOCUS       DASPGE010000357        12435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1732156, whole genome shotgun sequence.
ACCESSION   DASPGE010000357 DASPGE010000000
VERSION     DASPGE010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12435
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732156"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1846)
                     /gene="aceB"
                     /locus_tag="VD791_11555"
     CDS             complement(230..1846)
                     /gene="aceB"
                     /locus_tag="VD791_11555"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HXV08659.1"
                     /translation="MSSPTIPEGVQIAGTMTPEYAEILTPEALAFLAGLHRRFDPRRL
                     ELLQRRAQRQKALDGGQMPDFLPETKTVREADWKVAPVPADLQDRRVEITGPTDRKMV
                     INALNSGANMYMADFEDSNTPTWSNQIEGQLNMRDAVRGTIGFVSPEGKQYKLNEKVA
                     TLLVRPRGWHLDEKHLLVDGKPMSGSLFDFGLYFFHNAKERVRRGSGVYLYLPKMESH
                     LEARLWNDAFVFAQNELGVAQGTVKATVLIETILATFEMDEILYELREHSAGLNCGRW
                     DYIFSCIKKLRSRPDFILADRGQVTMTAPFMRAYSLLLIKTCHRRGAFAMGGMAAQIP
                     IKNDPAANEAALAKVRADKEREASDGHDGTWVAHPGLVALAKEAFDKVMPTPNQIHRR
                     RDDVKVTARELLDFQPKGPITEQGLRTNINIGIQYIGAWLAGTGCVPIFNLMEDAATA
                     EISRAQIWHWIRSPLGKLDDGRKVTRELFKELVPQELQRVRELLGERQYAAGKYPEGA
                     QMFEALTLRDDFVEFLTLPAYDYLVAHEHR"
     gene            2009..2800
                     /locus_tag="VD791_11560"
     CDS             2009..2800
                     /locus_tag="VD791_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08660.1"
                     /translation="MSKTKLIWAAVLAAAVSCAPGFAQMDEQELKQDAIARLIDSHDP
                     ALSVGIGRVYVKQAALLAARELLRERGRAAGLAPEQWNARTEPWRAAERELLQGMDEL
                     IQRKVARGMWVREAWSALAAQTLNAEEADEIAVHFQTEGGQLQRRVIEWFVGELTLQT
                     YTFTDRLKYGVPGSEQEMRDLQIATYERMFRGIQDFTDYPGAMRFASQDPGVKYMKMM
                     VLQGVHAVHVHLEQVADEARATIRARAALTDPHIARARSAARARS"
     gene            complement(2831..4120)
                     /gene="aceA"
                     /locus_tag="VD791_11565"
     CDS             complement(2831..4120)
                     /gene="aceA"
                     /locus_tag="VD791_11565"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HXV08661.1"
                     /translation="MTPEQTRQQQIARIQQDWADNPRWKGVRRGYTAQDVYRLRGSLH
                     IEHTLARRGAEKLWRLLAEEPFVGALGALTGNQALQQVKAGLKAIYLSGWQVAGDAND
                     ACEMYPDQSLYPVSSVPTVVRRINNAFIRADQIHHAEGKDDIDWFAPIVADAEAGFGG
                     VLNAHELMKHMIEAGAAGVHFEDQLAAAKKCGHMGGKVLVPTQEAVQKLIAARLAADL
                     AGVPTVLFARTDAEAASLITSDIDANDRPFLTGERTAEGFFHTRNGLAQAVSRGLAYA
                     PYADLIWCETGKPDLEFARDFAEAIHAVHPDKLLAYNCSPSFNWKKNLDDATVAKFQR
                     ELGAMGYKFQFITLAGFHALNYSMFDLAHGYAREHMSAFVKLQQAEFAAEARGYTATK
                     HQREVGTGYFDAITQAVQGGQSSVTALTGSTEEEQFA"
     gene            4348..5601
                     /gene="icd"
                     /locus_tag="VD791_11570"
     CDS             4348..5601
                     /gene="icd"
                     /locus_tag="VD791_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HXV08662.1"
                     /translation="MSKHIRVPNQGEKIIVNADSTLKVPANPIVPFIEGDGTGVDITP
                     VMRSVVDAAVSIAYGGKRKIVWMEMYAGEKSCRVYGENVWLPDETLAAAREFVVSIKG
                     PLTTPVGGGIRSINVALRQELDLYVCLRPVRYFKGVPSPLKQPEKTDMVIFRENSEDI
                     YAGIEWQAESEGARKVVKFLIEDMGVKKIRFPQTSGIGVKPVSREGTERLVRKAIQYA
                     IANKRRSVTLVHKGNIMKFTEGAFRDWGYALAQREFGGQPLDGGPWVKLPEKHGALVI
                     KDVIADAFLQQILLRPDEYDVIATLNLNGDYISDALAAQVGGIGIAPGANTSDSVAMF
                     EATHGTAPKYAGKDYVNPGSLILSAEMMLRHMSWTEAADLIVASMEKTILDQVVTYDF
                     ARLMQGARQVSCSGFGQAMVERMRA"
     gene            complement(5857..6060)
                     /locus_tag="VD791_11575"
     CDS             complement(5857..6060)
                     /locus_tag="VD791_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HXV08663.1"
                     /translation="MATGTVKWFNDSKGYGFITPDDGGEDLFAHFSAINMSGFKTLKE
                     GQKVSFEVTQGPKGKQASNIQAA"
     gene            6329..6637
                     /gene="clpS"
                     /locus_tag="VD791_11580"
     CDS             6329..6637
                     /gene="clpS"
                     /locus_tag="VD791_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HXV08664.1"
                     /translation="MASGNRDGALLEAKKSRVKPPPMYKVLLLNDDYTPMDFVVAVLQ
                     TFFSMTREQATQIMLKVHREGMGVCGVYPKDVAATKVEQVTAFSRKHQHPLQCLMEET
                     "
     gene            6725..8998
                     /gene="clpA"
                     /locus_tag="VD791_11585"
     CDS             6725..8998
                     /gene="clpA"
                     /locus_tag="VD791_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HXV08665.1"
                     /translation="MIAQELEVSLHMAFMDARQKRHEFITVEHLLLALLDNPSASEVL
                     RACAANIEELRKLLTDFINEHTPIVTGDEVDTQPTLGFQRVIQRAILHVQSSGKKEVT
                     GANVLVAIFGEKDSHAVYFLHQKGVTRLDVVNFISHGISKVPQTGPAKQQESEADGEQ
                     EQQQGGALETYTMNLNALALAGKIDPLIGRERELERVIQTLCRRRKNNPLLVGEAGVG
                     KTAIAEGLARRIIEGDVPEILARCQVYALDMGALLAGTKYRGDFEQRLKAVLKQLVDN
                     PNAILFIDEIHTLIGAGAASGGTLDASNLLKPALSTGALKCIGATTYIEYRGIFEKDH
                     ALSRRFQKIDVTEPSVQETVEILRGLKSRFEAHHGVRYTVSALTSAAELSARYINDRH
                     LPDKAIDVIDEAGAAQRILPKSKQRRVITKHEIEEIIAKIARIPPHNVSSNDRSKLQN
                     LDRDLKAVVFGQDKAIEALAAAIKMARSGLGNPQKPIGCFLFSGPTGVGKTEVARQLA
                     YIMGVELIRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAITKHPYSVLLLDEIE
                     KAHPDIFNILLQVMDHGTLTDNNGRKADFRNVVIVMTTNAGAESLTKTSMGFTQGKTV
                     GDELQDIKRMFTPEFRNRLDATISFAALTHEVILRVVDKFLMQLEDQLHEKKVEASFT
                     EALKDYLAKHGFDQLMGARPMARLIQDTIRRALADELLFGRLANGGKVTVDIDAGGKI
                     KLEFAQEEPAAPASVEG"
     gene            9159..9464
                     /locus_tag="VD791_11590"
     CDS             9159..9464
                     /locus_tag="VD791_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV08666.1"
                     /translation="MTIQRLFPLALLGLAALAAAPLQAEDKAARSMAATCTGCHGTNG
                     VSSGGIPSIAGVEKERIATLMREFRDGKRPATVMHQHAKGYTDEQIEAIAAWFAERK"
     gene            9529..10767
                     /locus_tag="VD791_11595"
     CDS             9529..10767
                     /locus_tag="VD791_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
                     /protein_id="HXV08667.1"
                     /translation="MSAAGAGAAAGVPLYARAAGGRVVVVGGGFGGATAAKYIRMWAP
                     DIAVTLIERNREFISCPLSNRVLAGQMSLQDLTRGYGTLASKYGVKVVHDEVVEIDGD
                     KRQVKTRSGATLGYDRVIVSPGVDLLFDEIPGLKSAAARDKILHAWKAGPQTVALRKQ
                     LEAMRDGGVYAISVPKAPYRCPPGPYERACQVAWYFKTAKPKSKVLILDANDKVTSKE
                     GLFTKVWKEMYAGMVEYRPNSTLVDVDVASNTAKLEFEDVKADVLNVVPPQRAADIAL
                     STGLITANDKWCGVDYQSFESTARKNIHVLGDAIVAAPMMPKSGHMANQHGKVCAAAV
                     IELLQGGQVNPSPMVTNTCYSFVSDKEVIHVASVHRFDAEKKTIVTVQGSGGVSSAPN
                     EMEGSFAEAWGRNIWADMLG"
     gene            complement(10869..11813)
                     /locus_tag="VD791_11600"
     CDS             complement(10869..11813)
                     /locus_tag="VD791_11600"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HXV08668.1"
                     /translation="MGAFLAGCAARAPTLSALGSSAAPAQLELADVPFFPQEAYQCGP
                     AALATVLTYSGSPTTPEALAPRVYLPGRQGSLQVELVAAVRREDRLPYVIQASLAALM
                     AELQAGRPVVVLQNLGLSRLPTWHFAVVIGYSKQDDAVVLRSGTRQRVNMSAYRFART
                     WEPGGRWGLVVLRPGELPAADDADGYLRAVAAKEAITGPAGLVQAYRAAIERWPESTL
                     ARFGLANALRANGQVAAAVAQYRVLVSESPGDAAALNNLADALNALGCREPALQTIER
                     ALALMPELDPRREVALQTRQEILSAPQAGSAAAVACDP"
     gene            complement(11858..12235)
                     /locus_tag="VD791_11605"
     CDS             complement(11858..12235)
                     /locus_tag="VD791_11605"
                     /inference="COORDINATES: protein motif:HMM:NF042483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HXV08669.1"
                     /translation="MMHPFLRRFVAILAAVSMPICGQVHTAQAAVIGTDTVVQLADRT
                     QAISRIEAQLARDEVQQAFIRLGVDPGEAKARVSVLGDAEIAQLDRELAKLPAGGDGG
                     WTFAVVLLIVLVVLFAMGKLQYR"
BASE COUNT         2088 a         4126 c         4270 g         1951 t
ORIGIN      
        1 cactcaagaa gaacctcaag gacccggaga cctgggcgca cctcaaggat ctgcgcgagg
       61 agctggcgcg cgcgctgtaa ggcttcaccg caaaggcgca aaggcgcgga gggagcgcaa
      121 agaaagacgc gcgacatccg cgccgtcgag actctcgctt gagctttttc tctgcgcccc
      181 tttgcgcctt tgcggttccg gcgtagcgca ccccgcgtcg cggggagcct caccggtgct
      241 cgtgtgccac caggtagtcg tacgcgggca gcgtgaggaa ctcgacgaag tcgtcgcgca
      301 gggtgagcgc ctcgaacatc tgcgcccctt ccgggtactt cccggccgcg tactggcgct
      361 cgccgagcag ctcgcgcacg cgctgcagct cctggggcac cagttccttg aacagctccc
      421 gggtgacctt gcggccgtcg tcgagcttgc ccagggggct gcggatccag tgccagatct
      481 gggcgcgtga gatctcggcg gtggccgcgt cctccatcag gttgaagatc ggcacgcagc
      541 cggttccggc cagccaggcg ccgatgtact ggatgccgat gttgatgttg gtgcgcaggc
      601 cctgctcggt gatcgggccc ttgggctgaa agtcgagcag ctccctggcg gtcaccttca
      661 cgtcgtcgcg ccgccggtgg atctggttgg gggtcggcat gaccttgtcg aacgcctcct
      721 tggcgagtgc caccaggccg gggtgcgcca cccaggtgcc gtcgtgcccg tcgctggcct
      781 cgcgctcctt gtcggcgcgc accttggcga gcgccgcctc gttggcggcc ggatcgttct
      841 tgatcgggat ctgcgccgcc atcccgccca tggcgaaggc cccgcgccgg tggcaggtct
      901 tgatcaggag cagcgagtag gcgcgcatga acggcgcggt catggtgacc tggccgcgat
      961 cggcgaggat gaaatcgggc cggctcctga gcttcttgat gcagctgaag atgtagtccc
     1021 agcggccgca gttgagcccc gcggagtgct cgcgcagctc gtacaggatc tcgtccatct
     1081 cgaaggtcgc gaggatggtt tcgatcagca cggtggcctt caccgtgccc tgcgccacgc
     1141 ccagctcgtt ctgagcgaac acgaacgcgt cgttccacag ccgcgcctcg agatggctct
     1201 ccatcttggg cagatacagg tacacgcccg agccgcgccg cacccgctcc ttggcgttgt
     1261 ggaagaagta caggccgaag tcgaacagcg agcccgacat cggcttgccg tccaccagca
     1321 ggtgcttctc gtccaggtgc cagccgcgcg ggcgcaccag cagcgtggcg actttttcgt
     1381 tgagcttgta ctgcttgccc tccgggctga cgaagccgat cgtgccgcgc accgcgtcgc
     1441 gcatgttgag ctggccttcg atctggttgc tccaggtggg cgtgttggag tcctcgaagt
     1501 cggccatgta catgttggcg cccgagttga gcgcgttgat caccatcttg cggtccgtgg
     1561 gcccggtgat ctccacgcgc cggtcctgca ggtcggcggg caccggcgcg accttccagt
     1621 cggcctcgcg gacggtcttg gtctcgggca gaaagtccgg catctgcccg ccgtcgagcg
     1681 ccttctggcg ctgcgcccgg cgctgcagca gctccagccg gcgcgggtcg aaccggcggt
     1741 gcaggccggc gagaaaagcg agcgcttccg gagtgaggat ctcggcgtac tcgggcgtca
     1801 tggtgcccgc gatctgaacg ccttcgggga tggtcgggct ggacatggtc tggcttgccg
     1861 atcgggtttg cgttgggtca agggatagcg cgattctcgc acataaactc catcctcaag
     1921 gtgcgctcac taccgctcgc tccctgccgc gggagccgct ggtgagatgc gtctatccga
     1981 gtgcaggttg tcgccgtctg gaggtcgagt gtcgaagacg aagctgatct gggctgccgt
     2041 gctggccgcc gccgtgtcgt gcgcgccggg gttcgcgcag atggacgagc aggagttgaa
     2101 gcaggacgcg atcgcgcgcc tgatcgacag ccacgatccg gcgctgtcgg tcggaatcgg
     2161 gcgcgtgtac gtgaagcagg cggcgctgct ggccgcgcgc gagctgctgc gcgagcgcgg
     2221 acgggccgca ggcctggcgc ccgagcaatg gaacgcgcgc accgagccct ggcgcgcggc
     2281 cgagcgcgag ctgctgcagg gcatggacga gctgatccag cgcaaggtcg cgcgcggcat
     2341 gtgggtgcgc gaggcgtggt cggcgctggc cgcgcagacc ttgaacgcgg aggaagccga
     2401 cgagatcgcg gtgcacttcc agaccgaggg cgggcagctg cagcggcggg tgatcgaatg
     2461 gttcgtcggc gagctgaccc tgcagaccta caccttcacc gatcggctga agtacggggt
     2521 gccgggttcg gagcaggaga tgcgcgacct gcagatcgcc acctatgagc ggatgttccg
     2581 cggcatccag gacttcaccg actatccggg ggcgatgcgc ttcgccagcc aagaccccgg
     2641 cgtgaagtac atgaagatga tggtcctgca gggcgtgcac gcggtgcacg tgcatctgga
     2701 gcaggtggcc gacgaagcgc gggccacgat ccgcgcgcgc gccgctctca ccgacccgca
     2761 catcgcgcgc gcgcgcagcg ccgctcgcgc ccgcagttga cgcgttcgcg cggcggcgct
     2821 cgccggggac tcaggcgaac tgctcttcct cggtggaccc ggtgagcgcg gtcacggacg
     2881 actgcccgcc ctgcaccgcc tgcgtgatgg cgtcgaagta gccggtgccc acctcgcgct
     2941 ggtgcttggt ggcggtgtag ccgcgcgcct cggccgcgaa ctccgcctgc tgcagcttca
     3001 cgaacgcgct catgtgctcg cgcgcgtagc cgtgcgccag gtcgaacatg ctgtagttca
     3061 gggcgtggaa gccggccagc gtgatgaact ggaacttgta gcccatcgcc cccagctcgc
     3121 gctggaactt cgccacggtg gcgtcgtcca ggttcttctt ccagttgaac gacggcgagc
     3181 agttgtaggc cagcagcttg tccgggtgaa cggcgtggat cgcctcggcg aagtcgcgcg
     3241 cgaactccag atcgggcttg ccggtctcgc accagatcag gtccgcgtac ggcgcgtagg
     3301 ccagcccgcg ggagacggcc tgcgccaggc cgttgcgcgt gtggaagaag ccctccgcgg
     3361 tgcgctcgcc ggtgaggaac ggccggtcgt tggcgtcgat gtcggacgtg atcaggctgg
     3421 cggcctcggc gtcggtgcgc gcgaacagca ccgtcggcac gccggcgagg tcggccgcaa
     3481 gccgcgccgc gatcagcttc tgcaccgcct cctgggtggg caccagcacc ttgccgccca
     3541 tgtggccgca cttcttggcg gcggcgagct ggtcctcgaa atgcaccccg gcggcgcccg
     3601 cctcgatcat gtgcttcatc agctcgtggg cgttgagcac gccgccgaag cccgcctcgg
     3661 cgtcggccac gatcggcgcg aaccagtcga tgtcgtcttt gccctcggcg tgatggatct
     3721 gatcggcgcg gatgaaggcg ttgttgatcc tgcgcaccac cgtgggaacg ctgctcaccg
     3781 ggtacagcga ctgatccggg tacatctcgc aggcgtcgtt ggcatcgccc gccacctgcc
     3841 agccggacag gtagatggcc ttgaggccgg ccttcacctg ctgcagcgcc tggttgccgg
     3901 tcagcgcgcc cagcgcgccg acgaagggct cctcggcgag cagccgccac agcttctcgg
     3961 ccccgcgccg tgccagcgtg tgctcgatgt gcaggctccc gcgcaggcgg tacacgtcct
     4021 gcgccgtgta gccgcggcgc acgcctttcc agcggggatt gtcggcccag tcctgctgga
     4081 tccgtgcgat ctgttgctgc cgcgtctgct cgggggtcat cggaggtctc cagaaaatgc
     4141 cgcaactgac gagaaaaacc agtatagcaa tcgcatggcg cgacgcaaca tggcggcgtt
     4201 gcgcccggtg cgggcctgga cgggaaacgc tgccgcattg caaaacgcac gggtccgcgg
     4261 cggcgccggg cgggcccctc gccggggccg ggatgccggt gtagaatcct tctttttcaa
     4321 cagcctacaa cacctcccaa ggagcctatg agcaagcaca tccgggtgcc gaaccaaggc
     4381 gagaagatca ttgtcaacgc ggactccacc ctgaaggtcc ccgccaaccc catcgttccg
     4441 ttcatcgagg gcgacggcac cggggtggac atcacgccgg tcatgcgcag cgtggtggac
     4501 gcggcggtga gcatcgccta cggtggcaag cgcaagatcg tctggatgga gatgtacgcc
     4561 ggcgagaagt cctgccgggt ctacggcgag aacgtctggc tgcccgacga gacgctggcc
     4621 gccgcccggg agttcgtcgt gtccatcaag ggcccgctta caacgcccgt aggcgggggc
     4681 atccgctcga tcaacgtcgc cctgcgccag gagctggacc tgtacgtgtg cctgcgcccg
     4741 gtgcgctact tcaagggcgt gcccagcccg ctcaagcagc ccgagaagac cgacatggtg
     4801 atcttccgcg agaactcgga ggacatctac gccggcatcg agtggcaggc cgagagcgag
     4861 ggcgcgcgca aggtcgtcaa gttcctgatc gaggacatgg gcgtgaagaa gatccgcttc
     4921 ccccagacct cgggcatcgg cgtcaagccg gtctcgcgcg agggcaccga acgcctggtg
     4981 cgcaaggcca tccagtacgc catcgccaac aagcgccggt cggtgaccct ggtgcacaag
     5041 ggcaacatca tgaagttcac cgagggcgcg ttccgcgact ggggctacgc gctcgcccag
     5101 cgcgagttcg gcggccagcc gctcgacggc gggccctggg tgaagctgcc cgagaagcac
     5161 ggcgctctgg tgatcaagga cgtgatcgcc gacgctttcc tgcagcagat cctgctgcgg
     5221 cccgacgagt acgacgtgat cgcgacgctg aacttgaacg gcgactacat ctcggatgcg
     5281 ctggcggcgc aggtgggcgg catcggcatc gcgcccggcg ccaacaccag cgattcggtg
     5341 gccatgttcg aggccaccca cggcaccgcg cccaagtacg cgggcaagga ctacgtgaac
     5401 ccgggctcgc tgatcctttc ggcggagatg atgctgcggc acatgagctg gacggaggcc
     5461 gccgacctga tcgtcgcgtc gatggagaag accatcctgg accaggtggt cacctacgac
     5521 ttcgcacgcc tgatgcaggg tgcgaggcag gtgtcgtgct ccggcttcgg acaggcgatg
     5581 gtggagcgca tgcgcgcgta ggcaggagga aggcttgtct ctatccccgg attgcgcttc
     5641 gctccatccg ggcaccgggc gggccgccga gtcccggtcc gtcaaaaaaa caaaacccgc
     5701 ttcgtgggcg ggtttggatt attctccctg gacggagtgg atgcactccg cctcgaatcc
     5761 gaactggatg cctcctcggg actcctgctt tggggaaacc ctgcttgggg aaccgctgac
     5821 ggtgtgtcag ggtgactcgt ggtaggcggc tcgcgctcac gccgcctgaa tgttggaagc
     5881 ctgcttgcct ttcggcccct gtgtgacttc gaagctgacc ttctggccct ccttgagcgt
     5941 cttgaaaccg ctcatgttga tggccgagaa gtgggcgaac agatcctccc caccgtcgtc
     6001 gggcgtgatg aaaccgtagc ccttcgagtc gttgaaccac tttaccgtac cagtcgccat
     6061 acttcctctt ccttgtcttc cttgtgggag atctggcgcg ggctgcatgc ccgtgcggcg
     6121 cgcgagcgcc gcggcggcgt acccggcccg catacgggca ggcgccatgc gccgatgttc
     6181 tttttaggcc attcaccacc tgaagtcaag gaaccggccg gcgcccggac cggtacgcaa
     6241 aatgcttcaa tgcacttgaa aattctgtgc tattgcgcga aattagcgtg cagcccatgt
     6301 tcggtcggct tgcgcttcgg aagtagccat ggctagtgga aaccgcgacg gcgccctgct
     6361 ggaggccaag aagagccgcg tcaaaccgcc gccgatgtac aaggtgctgc tgctcaacga
     6421 cgattacacg ccgatggatt tcgtggtcgc cgtgctgcag acgttcttct cgatgacccg
     6481 cgagcaggcc acgcagatca tgctcaaggt gcaccgcgaa ggcatggggg tgtgcggggt
     6541 ctaccccaag gacgtcgccg ccaccaaggt cgagcaggtg accgccttct cccgcaagca
     6601 ccagcacccc ctgcaatgcc tgatggagga gacgtgaccc tgtcaacgga aagtcacccg
     6661 acgggcgcgg cacacggttt cggtggtaca gtagcgtcag ctgctgttgg ctagaggata
     6721 gataatgatc gcgcaggagc tagaggtcag cctgcacatg gcattcatgg acgcccgcca
     6781 gaagcgccac gagttcatca ccgtggagca tctgctgctg gcgctgctgg acaacccgtc
     6841 ggcgtccgaa gtgctgcgcg cctgcgccgc caatatcgag gagctgcgca agctgctcac
     6901 cgacttcatc aacgagcaca ccccgatcgt caccggcgac gaggtcgaca cccagcccac
     6961 gctcggcttc cagcgcgtga tccagcgcgc catcctgcac gtgcagtcct ccggcaagaa
     7021 ggaggtgacc ggcgccaatg tcctggtggc gatcttcgga gagaaggatt cgcacgccgt
     7081 ctacttcctg caccagaagg gcgtcacgcg cctggacgtg gtgaacttca tctcgcacgg
     7141 tatctccaag gtgccgcaga ccggtccggc caagcagcag gagagcgagg ccgacggcga
     7201 gcaggagcag cagcagggcg gcgcgctcga gacctacacc atgaacctca acgcgctggc
     7261 gctggcgggc aagatcgacc cgctgatcgg ccgcgagcgc gagctggagc gggtgatcca
     7321 gacgctgtgc cggcggcgca agaacaaccc gctgctggtg ggcgaggccg gggtgggcaa
     7381 gaccgccatc gccgagggcc tggcgcggcg catcatcgag ggcgacgtgc ccgagatcct
     7441 ggcgcgctgc caggtgtacg cgctggacat gggtgcgctg ctggcgggca ccaagtatcg
     7501 gggcgatttc gagcagcgtc tcaaggccgt gctcaagcag ctggtggaca accccaacgc
     7561 gatcctgttc atcgacgaga tccacaccct gatcggcgcg ggagcggcct cgggtggcac
     7621 gctggacgcc tccaacctgc tcaagccggc gctgtccacc ggcgcgctga agtgcatcgg
     7681 cgccaccact tacatcgagt accgcggcat cttcgagaag gaccacgcgc tgtcgcgccg
     7741 cttccagaag atcgacgtga ccgagcccag cgtgcaggag acggtggaga tcctgcgcgg
     7801 gctgaagagc cgcttcgagg cgcaccacgg cgtgcgctac accgtttcgg cgcttacctc
     7861 cgcggccgag ctgtcggcgc gctacatcaa cgaccggcac ctcccggaca aggccatcga
     7921 cgtgatcgac gaggccggtg cggcgcagcg catcctgccc aagtccaagc agcggcgcgt
     7981 gatcaccaag cacgagatcg aggagatcat cgccaagatc gcgcgcatcc cgccgcacaa
     8041 cgtctcgtcc aacgaccgca gcaagctgca gaacctggac cgcgacctga aggccgtggt
     8101 gttcgggcag gacaaggcga tcgaggcgct cgccgcggcc atcaagatgg cgcgcagcgg
     8161 cctgggcaac ccgcagaagc cgatcggctg cttcctcttc tcggggccca ctggcgtggg
     8221 caagaccgag gtggcgcgcc agctcgccta catcatgggc gtggagctga tccgcttcga
     8281 catgtccgag tacatggagc ggcacgcggt gtcgcgcctg atcggcgcgc cgccgggcta
     8341 cgtcggcttc gaccaggggg ggctgctcac cgaggccatc accaagcacc cgtactcggt
     8401 gctgctgctc gacgagatcg agaaggcgca cccggacatc ttcaacatcc tgctgcaggt
     8461 gatggaccac ggcacgctga ccgacaacaa cgggcgcaag gcggacttcc gcaacgtggt
     8521 gatcgtcatg accaccaatg ccggcgcgga atcgctcacc aagaccagca tgggcttcac
     8581 ccagggcaag acggtaggcg acgagctgca ggacatcaag cgcatgttca cgcccgagtt
     8641 ccgcaaccgg ctggacgcga ccatctcgtt tgccgcgctc acccacgagg tgatcctgcg
     8701 cgtggtggac aagttcctga tgcagctcga ggaccagctg cacgagaaga aggtcgaagc
     8761 gagcttcacc gaggcgctca aggactatct ggccaagcac ggcttcgacc agctgatggg
     8821 cgcacggccg atggcgcgcc tgatccagga caccatccgc cgggcgctcg ccgacgagct
     8881 gctgttcggc cggctggcca acggcggcaa ggtcaccgtg gacatcgatg ccggcggcaa
     8941 gatcaagctc gagttcgccc aggaggagcc ggccgcgccg gcgagcgtgg aaggctgagc
     9001 cgcgcgggtc cgcgcgtcaa cgggcagccg cggctgcccg ttttcgtttt tcaggcccgc
     9061 ttgcgttgtt cagggataga atgcctgcca cggccggcgg gggagggcat gcgtccggcc
     9121 gccgcccgcg ccgggcggcc ccgacagaag gaggagaaat gaccatccag cgactgttcc
     9181 cgctcgccct cctgggcctg gctgcgctcg ccgcagcgcc gctccaggcc gaggacaagg
     9241 cggcgcgcag catggcggcg acctgtaccg gctgccacgg caccaacggt gtgagcagcg
     9301 gcggcatccc gagcatcgcc ggcgtcgaaa aggagcgcat cgcaaccctg atgcgggagt
     9361 tccgcgacgg caagcggccg gccacggtga tgcaccagca cgccaagggc tacaccgacg
     9421 agcagatcga ggcgatcgcc gcctggttcg ccgagcgcaa gtgacggcca tcggaaccag
     9481 catggggaga ggcagatgag cgtatccaga cgggagttct tgaaacggat gtccgccgcc
     9541 ggtgccggcg ccgcggccgg cgtgcccctg tatgcgcgcg cagcgggcgg gcgggtcgta
     9601 gtggtgggtg gcggcttcgg cggggcgacg gccgccaagt acatccgcat gtgggcgccg
     9661 gacatcgcgg tgacgctgat cgagcgcaac cgcgagttca tctcctgccc gctgagcaat
     9721 cgcgtgctcg cggggcagat gagcctgcag gacctgaccc gcggctatgg cacgctggcg
     9781 tccaagtacg gggtcaaggt ggtgcacgac gaggtcgtgg agatcgacgg cgacaagcgc
     9841 caggtgaaga cccggagcgg ggccacgctg ggctacgatc gcgtgatcgt ctcgcccggg
     9901 gtggacctgc tgttcgacga gattccgggc ctgaagtccg ccgcggcgcg cgacaagatc
     9961 ctgcacgcct ggaaggccgg cccgcagacc gtggcgctgc gcaagcagtt ggaggcgatg
    10021 cgcgacggcg gcgtctatgc gatcagcgta ccgaaggcgc cttaccgctg cccgcccggg
    10081 ccgtacgagc gcgcctgcca ggtggcctgg tacttcaaga ccgcgaagcc caagtccaag
    10141 gtgctgatcc tcgacgccaa cgacaaggtg acgtcgaagg aggggctgtt caccaaggtg
    10201 tggaaggaga tgtacgccgg catggtcgag taccggccga acagcacgct ggtggacgtg
    10261 gacgtggcca gcaacacggc caagctcgag ttcgaggacg tgaaggccga tgtcctgaac
    10321 gtggtgccgc cgcagcgcgc cgcggacatc gcgctcagca ccggcctcat caccgccaac
    10381 gacaagtggt gcggagtgga ttaccagagc ttcgagtcga ccgcgcgcaa gaacatccac
    10441 gtgctgggcg acgccatcgt cgctgcgccg atgatgccca agtccggcca catggcgaac
    10501 cagcacggca aggtctgcgc ggccgccgtc atcgagctgc tgcagggcgg tcaggtcaat
    10561 cccagcccga tggtcaccaa cacctgctac agcttcgtga gcgacaagga ggtgatccac
    10621 gtcgcctcgg tgcacaggtt cgatgcggag aagaagacca tcgtgacggt gcagggcagc
    10681 ggcggggtgt ccagcgcgcc caacgagatg gaaggcagct tcgccgaggc ctggggtcgc
    10741 aacatctggg ccgacatgct cgggtagccg ggtgcgcctg gtgcgccctt aaggcgcgaa
    10801 ttcaagaaaa gccgccccgc ccttcgccag acagcacggg gcgatgcggg cgcggcggtg
    10861 aggcccggct acggatcgca tgccacggcg gctgcggatc cggcctgtgg cgcggacagg
    10921 atctcctgcc tcgtctgcag cgccacctcg cgccgcgggt cgagttcggg catcagcgcg
    10981 agcgcgcgct cgatcgtctg cagcgccggt tcacggcagc cgagcgcgtt gagcgcgtcg
    11041 gcgaggttgt tgagcgcggc cgcgtccccg ggagattcgc tcaccagcac gcgatactgc
    11101 gcgaccgccg ccgccacttg cccgttcgcg cgcagcgcat tcgccaggcc gaagcgtgcg
    11161 agcgtgctct ccggccagcg ctcgatcgca gcccgatatg cctgcaccag gccggcgggg
    11221 ccggtgatcg cttccttggc cgcgaccgcc cgcagatagc cgtcggcatc gtccgctgca
    11281 ggcagctcgc cgggtcgcag caccaccagc ccccagcggc cgcccggctc ccaggttcgc
    11341 gcgaagcggt acgcgctcat gttgacgcgc tgcctggtgc ccgagcgcaa caccaccgcg
    11401 tcgtcctgtt tcgagtatcc gatcaccacg gcgaaatgcc acgtgggcaa gcgcgacagc
    11461 cccaggttct gcagcaccac gaccgggcgg ccggcctgca gctccgccat caatgccgcg
    11521 agcgatgcct ggatcacgta gggaaggcga tcctcccggc gtaccgccgc caccagctcc
    11581 acctgcaggc tgccttgccg gccgggcagg tacacgcgcg gggcgagcgc ttcgggtgtg
    11641 gtggggctgc ccgagtaggt gagcaccgtc gccagcgccg caggtccgca ttgatacgcc
    11701 tcctgcggga agaacggcac atccgccagc tcgagctgcg cgggcgcggc ggacgagccg
    11761 agcgcggaga gcgtcggcgc gcgcgccgcg caaccggcga gaaaggcgcc cacgaccagg
    11821 acgaccccgg gggcgagcgc cgggaaaaac gccggcttca gcggtattgc agcttgccca
    11881 tggcgaacag aacgaccagg acgatcagca ggacgaccgc gaacgtccag ccgccgtcac
    11941 cgccggccgg gagcttggcg agctcccggt cgagctgcgc gatctcggcg tcgcccagga
    12001 ccgatacccg cgccttggcc tcgccgggat cgacccccag ccggatgaac gcttgctgca
    12061 cctcgtcgcg cgccagttgc gcctcgatcc gggagatcgc ctgcgtccgg tcggcgagct
    12121 ggaccaccgt gtcggtgccg atgacggccg cctgtgcggt gtggacctgc ccgcaaatgg
    12181 gcatggacac ggccgcgaga attgcgacga agcgccgcag gaaaggatgc atcattgggt
    12241 tggtctccgg ttctcgtggt tgtcgtgcgc cgatgacact gccatcggcc gcctctaaaa
    12301 gatagccact ctacgcggtt tcggcaactc gcgaacccgc cggcaagccc cgtacaatga
    12361 tcctttcggc ccccccggcg ccatggatct gcctcgactg ctgggccccg cgcccttgtc
    12421 cgagcacctg cagcg
//
LOCUS       DASPGE010000358         3638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_92445, whole genome shotgun sequence.
ACCESSION   DASPGE010000358 DASPGE010000000
VERSION     DASPGE010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92445"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /gene="dctP"
                     /locus_tag="VD791_11610"
     CDS             <1..936
                     /gene="dctP"
                     /locus_tag="VD791_11610"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXV08670.1"
                     /translation="RLGTLAQGNTSPHRALLEMRDKWREAPGGGVRLTVYPGTMGSES
                     DMVRRMRVGQLNAALLSAVGLVQIERTASALQFMPMVYRDWKELDHVRDKVAPAAEAR
                     MLERGFVPLFWADAGWVRIFSRRPGLLPDDFRRMKMFVWAGDPEQVEMMKDIGFQPVS
                     IEVTDILPALRTGLIDAVPATPFFALAGQFAGPAPYMLDIKWVPIAGACVITRAAWDA
                     LSEPARAELKAAAQQAGAQIRSRARTEDLESIEALKGRGLKVSALTSEAQAQWRHLAE
                     QVIYPRIRDTKVPAPVFDETMRLLDEYRGRAPVEK"
     gene            945..2780
                     /locus_tag="VD791_11615"
     CDS             945..2780
                     /locus_tag="VD791_11615"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXV08671.1"
                     /translation="MSLAPVRAGASAAPRRRPAAAAEDFPLTVALGAMALLPIAEIGL
                     RALFHTGIPGTAVIVQHLTLLVGALGGAVAAREDRLLALSAAPLLMRGRLLKIARLVS
                     GCVAALVSLLMAVAGTNLVLAEHAAASQLLPHVPLWAVQSVLPLAFAVIAVRLIYHAG
                     SDWYTRLACALICATVMAAAGSLSLDAHALRIPALTALAIATILGAPVFTTLAGAALI
                     LFWSDGLPIASLAVDHYRMTVNPSLPAIPLFTLAGYLLAESGAPGRLVRTFQALVGGL
                     RGGAAAVAVGTCALFTALTGASGVTILALGALLMPLLLAARVSQRDALGLVTSAGSLG
                     VLLPPSLPLILYAVVGQLPMEELFLAALLPAAALMGVVGVFAACREPRRAQRRFDADE
                     ARRALWAAKWELALPVVAFGALFSGYATPVEAAAVTALYAFVVTVLVHRELSLVRDVP
                     HVVAECGLLVGGVLLILGVALGFTNYLVDAQVPDHAVDWVKGSISSRCAFLLALNAFL
                     LAVGCLMDVFSAIVVVVPLIVPIGLAFGIDPLHLGVIFLANMELGFLTPPVGMNLFFA
                     SYRFGKPMPEVCRAVLPYAVVLAAGVLLVTYIPSLTTWLPGLGEG"
     gene            2835..>3638
                     /locus_tag="VD791_11620"
     CDS             2835..>3638
                     /locus_tag="VD791_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08672.1"
                     /translation="MRETMHYLQTALAGLSALGLAAGCASVDPKSPSTSAAGAGDVAS
                     KPFVFLAIQPDSHARLKNAAAKVTKESKLVRGSTFEQSEADQADYTFRLGFEVREGSL
                     TAPEVIWGMTTIMLLTVYPATCNQHHYTLTADLEDRAGNRIKSYALYRSQRQFLWLFR
                     GPNCEVPTERRVQRVAQSMLAEVYGDVAKSGVLKRPGAAVAGAPAGPLVYLSVSRAQD
                     IVERATKATSPFARFTFDPAQASLADLSVHMDFSAHGGEPESFASLMGRG"
BASE COUNT          486 a         1210 c         1276 g          666 t
ORIGIN      
        1 cgtctgggga ctctggccca gggaaatacg tccccgcacc gtgctctgct ggagatgcgc
       61 gacaagtggc gcgaggctcc cggcggcgga gtccgcctga ccgtgtatcc cggcaccatg
      121 ggcagcgagt ccgacatggt ccggcgcatg cgcgtggggc agctcaacgc cgcgctgctc
      181 tcggcggtcg gcctggtgca gatcgaacgc actgcctccg cgctgcagtt catgccgatg
      241 gtctaccgcg actggaagga gctggaccac gtgcgcgaca aggtcgcgcc cgcggcggag
      301 gcgcgcatgc tcgaacgcgg cttcgtgccc ctgttctggg ccgacgcggg ctgggtgcgg
      361 atcttctcgc gcaggcccgg actgctgccc gacgatttcc ggcgcatgaa gatgttcgtc
      421 tgggcgggag accccgagca ggtggaaatg atgaaggaca tcggcttcca gccggtctcc
      481 atcgaggtca ccgacatcct gccggcgttg cgcaccggcc tgatcgacgc cgtcccggcg
      541 acgccgttct tcgcgcttgc cgggcaattc gccgggccgg cgccctacat gctcgatatc
      601 aagtgggtac cgatcgcggg ggcatgcgtg atcacgcgcg ccgcctggga tgcgctgtcc
      661 gagccggcgc gcgccgagct caaggcggcc gcgcagcagg ccggtgcgca gattcgaagc
      721 cgtgcgcgca ccgaggacct ggaatcgatc gaagcgctga aggggcgtgg gctcaaggtc
      781 agcgccttga ccagcgaagc gcaggcgcag tggcgccacc tggccgagca ggtcatctac
      841 ccccgcatcc gtgacaccaa ggttcccgcg ccggtgttcg acgaaacgat gcgcctgctg
      901 gacgaatacc gcggcagggc gccggtcgag aaatgagcgc gacgatgtcc cttgctcccg
      961 tgcgcgcggg cgcctccgcc gctccgcgcc gccgccccgc cgcggccgca gaggacttcc
     1021 cccttaccgt tgcgctcggc gcgatggcgc tgctgccgat cgcggagatc ggcctgcgtg
     1081 cgctgttcca caccgggatt ccgggcaccg ccgtcatcgt gcagcacctg acgctgctgg
     1141 tgggagcact gggtggggca gtggccgccc gcgaggaccg attgctcgcc ttgtccgcgg
     1201 ctccgctgct catgcgtggg cgtctgctga agatcgcgag gctcgtgagc ggctgcgtcg
     1261 cggccctcgt ctccctgctg atggccgtgg ccggcaccaa cctggtcctg gccgaacacg
     1321 cagccgcgag ccagctcctg ccgcatgttc ccctgtgggc ggtgcagagc gtgttgccac
     1381 tggcgtttgc ggtgatcgcc gtgcgcctga tctatcacgc aggctcggat tggtacacgc
     1441 gtttggcctg tgcgctgatc tgcgcgacgg tgatggcggc cgccggatcc ctgtctctgg
     1501 atgcgcatgc cctgcggatc ccggcgctga ccgcactggc gattgcaacc atcctcggcg
     1561 cgccggtatt caccacgctg gccggcgcgg cgctgatcct gttctggagc gacgggctcc
     1621 cgatcgcctc gctcgcggtg gatcactacc ggatgaccgt caacccctcg ctgccggcga
     1681 tcccgctgtt caccctggcc ggctatctgc tcgcggaaag cggcgcaccc ggacgtctgg
     1741 tgcgcacgtt ccaggcgctg gtgggtgggc tgcggggtgg cgcggcggcg gtggccgtgg
     1801 gcacgtgcgc gctgttcact gcgctgaccg gcgcatccgg ggtcaccatc cttgcgctcg
     1861 gcgcgctgct gatgccgttg ctgctggcgg cgcgcgtctc gcaacgcgat gcgctcgggt
     1921 tggtgaccag tgccggatcg ctgggcgtgc tgttgccgcc gagcctgccg ctgatcctgt
     1981 atgcggtggt gggccagctg ccgatggagg agctgttcct ggcggcgctg ctgcccgctg
     2041 cggcgctgat gggcgtggtc ggcgtctttg cagcatgccg tgagccccgg cgcgcgcagc
     2101 gacgattcga tgccgacgag gcccggcgcg cgctgtgggc ggcgaaatgg gaactggcgc
     2161 tgccggtggt ggccttcggt gcgctgttca gcggctacgc cacaccggtg gaagcggccg
     2221 cggtcaccgc cttgtatgcg ttcgtcgtaa ccgtcctggt gcatcgcgag ctcagcctgg
     2281 tgcgcgatgt accgcacgtg gtcgccgagt gcggcctgct ggtcggcggc gtgctgctga
     2341 tcctcggcgt cgctctgggc tttaccaact acctggtcga cgcccaggta cccgatcacg
     2401 cagtggactg ggtcaagggg tcgatctcgt ctcgttgcgc cttcctgctc gccctcaacg
     2461 cgtttctgct ggcggtgggc tgcttgatgg acgtgttctc cgccatcgtg gtggtggtgc
     2521 cgctgatcgt gcccatcggg ctcgccttcg gcatcgaccc gctgcatctg ggcgtcatct
     2581 tcctcgccaa catggagctg ggtttcctga ctccgccggt cgggatgaat ctgttcttcg
     2641 cctcctaccg cttcggcaag ccgatgccgg aagtgtgtcg ggcggtgttg ccatacgcgg
     2701 tggtgctggc tgcgggcgtg ctgctcgtga cttacatccc ttccctcacc acctggcttc
     2761 cgggcctggg cgagggttga gcgaaggctc gaggctgccg acgaccggca attgcgcagc
     2821 aagaggagac ggtcatgcgt gaaaccatgc actacctaca gacggcgctc gcggggctgt
     2881 ctgccttggg gctcgccgcg gggtgcgcgt ccgtggatcc gaagagtcct tccaccagcg
     2941 cggccggcgc gggcgatgtc gcctccaaac ccttcgtctt tctggccatc cagcccgatt
     3001 cgcacgcgcg cttgaaaaac gcagccgcga aggtcacgaa agagtcgaag ctggtgcggg
     3061 ggtcgacctt cgagcagagc gaggctgacc aggccgacta cacattccgg ctcggcttcg
     3121 aggtgcgtga gggttcgttg acggcgcctg aagtgatatg gggcatgacc acgatcatgc
     3181 tgctcacggt ttatcccgcg acctgcaacc agcaccacta cacgttgacc gcggacctgg
     3241 aagaccgcgc cggcaaccgc atcaagtcct atgcactgta ccgatcgcag cggcagttcc
     3301 tctggctgtt ccgcggcccg aactgcgagg ttccgaccga gcgcagggtg cagcgggtcg
     3361 ctcaatcgat gctggccgag gtctacggcg acgttgcgaa gagcggtgtc ctgaagcggc
     3421 caggtgccgc ggtggccgga gcaccggcgg gacctctggt gtatctgtcg gtgtcgcgcg
     3481 cacaggatat cgtcgaacgg gccaccaagg cgacctcgcc cttcgcgcgc ttcacattcg
     3541 atccggcgca ggcgtccctg gccgacctct cggtgcatat ggatttctcg gctcacggtg
     3601 gcgaaccgga gtcgtttgca tcgctgatgg gccggggg
//
LOCUS       DASPGE010000359         4569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_327886, whole genome shotgun sequence.
ACCESSION   DASPGE010000359 DASPGE010000000
VERSION     DASPGE010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327886"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VD791_11625"
     CDS             <1..1005
                     /locus_tag="VD791_11625"
                     /inference="COORDINATES: protein motif:HMM:NF015078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08673.1"
                     /translation="VQVYRQIYLLALIIPVVSVFGTVLAGILRRGTERRLRAQGFGAA
                     DIDRIVHGGHVRPQVNWWILGGSLVFVAFTVAMGLSDFAYSQEVVFLGSLGIIVFLMT
                     RLTGELDPVARNTLIGTAIVIFVFRAIPGPGAGSSWWEIDVLGFDQQFLSKLALITST
                     LTLLGMFLFRRFMAEHSIVYVIGFLTLAGALLFLPSVAMYYGFHEWTAARTGGVVDQR
                     FIAVLNTAIESPLGQIAMIPMLAWIAHSAPDRLKATFFAVMAAFTNLALSLSQLGTKY
                     LNQIYTVTREVKDPVTGAIKVAADYSQLGHLFITVLVIAVVAPLLAILFVKTTRFRSA
                     "
     gene            1203..2027
                     /locus_tag="VD791_11630"
     CDS             1203..2027
                     /locus_tag="VD791_11630"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXV08674.1"
                     /translation="MLSMLASGFAAAARPVSAQTVLTDVKGLTAGEIEFSTPDRPIPA
                     YHAMPATGGPFPIVLVVQEIFGVHAHIRDVCRRLAKVGYLAIAPELFVRQGDVSALSD
                     IQQILKDVVSRVPDKQVTSDLDAAVGWAAALGRGDPGRLGITGFCWGGRVTWLYCMHN
                     RTVKAGVAWYGRLVGQETELTPRHPLDIAAKLSVPVLGLYGGADAGIPQETVGRMRDA
                     LRKGASGSEIFVYPDAPHAFFADYRPSYRKKAAEDGWERMLAWFKRHGVEVPAVEG"
     gene            2049..2432
                     /locus_tag="VD791_11635"
     CDS             2049..2432
                     /locus_tag="VD791_11635"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXV08675.1"
                     /translation="MNRTIDAGVAARIGTYSDAVEIAPGKRLVFVSGTPGIDPVSGQL
                     PESFVAQAELAWRNVHTILAQAGMGVEDIVKLTQYLVRREDLADYREIRSRNLREHRP
                     ASMLSFVDQLVWPNMLIELEVVAAQ"
     gene            complement(2461..3669)
                     /locus_tag="VD791_11640"
     CDS             complement(2461..3669)
                     /locus_tag="VD791_11640"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV08676.1"
                     /translation="MLREAWSAMGANRLRTFLTMLGLVIGVGAVVLMMAIGQGAQTLV
                     REAVATMGSNLFIVLSGGTSAGGARTGSGTAPTLTLADADAIGELASVAAVAPTQFGN
                     AQLVYGAANWNTQVMGVTPSYFEVREWRLVAGQPFTEADVRAVARVALLGQTVAKQVF
                     GDEDPVGKTVRIRNSPYLVLGVLASKGQSFDGRDQDDTVLVPLTTAQRKLYGSPFPGT
                     VRFMMIKALSEEAMPRAERAIDALLRARHRIPPDGEADFTIRNLSAVADVQAQTTRVM
                     SIMLGAVASVSLVVGGIGIMNIMLVSVTERTREIGIRMAIGARRRDILLQFLLESLIV
                     SVIGCLIGVILGVAAALLVSRVTQMTVVVTAVAIGLAFLVAAAVGVFFGFYPARKAAD
                     LKPIEALRYQ"
     gene            complement(3678..4421)
                     /locus_tag="VD791_11645"
     CDS             complement(3678..4421)
                     /locus_tag="VD791_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV08677.1"
                     /translation="MDRAAASAPLILIEHLHKQYETDAGPQPVLRDVSLRIEPGEFVA
                     VMGPSGSGKSTFMNILGCLDVASSGRYLLQGNDTSRLSGDALARLRNRFIGFVFQGFN
                     LLPRATLLDNVALPLLYAGVPVRSREERAHELLARVGLERYAAYLPSRISGGQQQRVA
                     IARALINSPRLVLADEPTGNLDSATSHDIMRLIAGLNAEHGTTVVLVTHEADVARFGK
                     RVIHFVDGVVDSDEIRGAAPASHALQASL"
BASE COUNT          685 a         1553 c         1593 g          738 t
ORIGIN      
        1 gtgcaggtct atcggcagat ctacctgctc gcgctgatca tcccggtggt atcggtgttc
       61 ggcaccgtgc tggccggcat cctgcggcgc ggtaccgagc gccgcttgcg ggcgcagggg
      121 ttcggcgcgg cggatatcga ccgcatcgtg cacggcggcc acgtgcgccc gcaagtcaac
      181 tggtggattc taggcggcag cctggtgttc gtggcgttca ccgtggccat ggggctgagc
      241 gatttcgcgt acagccagga ggtggtgttc ctcggatcgc tggggatcat cgtgttcctg
      301 atgacccggc tcaccggcga gctcgatccc gtggcgcgca acacgctgat cgggacggcg
      361 atcgtgatct tcgtgttccg cgccattccc ggccccgggg cgggctccag ctggtgggag
      421 atcgacgtgc tcggcttcga ccagcagttc ctgtccaagc tcgcgctcat caccagcacg
      481 ctcacgctgc tgggcatgtt cctgttccgg cgcttcatgg cggagcattc catcgtttac
      541 gtcatcggct tcctcaccct cgccggcgcg ctgctgttcc tgcccagcgt ggcgatgtac
      601 tacgggttcc acgagtggac cgccgcgcgc accggcgggg tggtcgatca gcgcttcatc
      661 gcggtgctca acaccgcgat cgagtcgccc ctggggcaga tcgcgatgat cccgatgctg
      721 gcgtggatcg cgcactccgc gcccgaccgg ctgaaagcga ccttcttcgc ggtgatggcg
      781 gcgttcacca acctcgcgct gtcgctgtcc cagctcggca ccaagtacct gaaccagatc
      841 tacacggtca cgcgcgaagt gaaggatccg gtcacggggg cgatcaaggt cgccgccgac
      901 tacagccagc tcggccacct gttcatcacc gtgctggtga tcgcggtggt ggcgccgctg
      961 ctcgcgatcc tgttcgtgaa gaccacccgc ttcaggagcg cgtgagcgcc ggttgaagaa
     1021 atgcgggttg gccggggttg aaccggccgc gccgatgcac taagttgtgc ttgctgaggt
     1081 tctcggaatt gcgcgacaag aaccacacca cccccggcct tcggccgacc cctcctcttg
     1141 agaggagggg agttgccttt ctcttctgcg gacgacgcgt cgagtggacg cgccgcgagt
     1201 tcatgctcag catgctggcg agcgggttcg ccgcggcggc gcggccggtg tcggcgcaga
     1261 ccgtgctgac cgacgtgaag ggcctgaccg cgggggagat cgagttctcc acgccggacc
     1321 ggccgatccc cgcctatcac gccatgcccg ccaccggcgg gccgtttccg atcgtgctgg
     1381 tggtgcagga gatcttcggc gtccacgcgc acatccgcga cgtctgccgg cgcctggcga
     1441 aggtcggtta cctggccatc gcgcccgagc tgttcgtgcg ccaaggcgac gtctcggcgc
     1501 tgagcgatat ccagcagatt ctcaaggacg tggtctcccg ggtgccggac aagcaggtga
     1561 caagcgatct ggatgcggcg gtggggtggg ccgcggcgct cgggcgcggc gatccgggtc
     1621 ggctgggcat caccgggttc tgctggggcg gccgcgtcac ctggctgtac tgcatgcaca
     1681 accgcacggt gaaggcgggc gtcgcgtggt acgggcgcct ggtggggcag gaaaccgagc
     1741 tcaccccgcg ccacccgctg gacatcgctg cgaagctgag cgtgccggtg ctcggcctgt
     1801 atggcggcgc cgacgccggc attccgcagg agaccgtcgg gcgaatgcgc gatgcgctac
     1861 ggaagggcgc cagcggatcg gagatcttcg tgtatccgga cgcgccgcac gccttcttcg
     1921 ccgactatcg cccgagctat cgcaagaagg ccgccgagga cggttgggag cgcatgctcg
     1981 cctggttcaa gaggcacggc gtcgaggtgc cggcggtgga gggatgatgt tgatactgga
     2041 ggactggaat gaatcgaacc atcgacgccg gcgtcgcggc gcgcatcggc acctacagcg
     2101 acgcggtcga gatcgctccc ggcaagcgcc tggtgttcgt atccggcacc ccgggcatcg
     2161 atcccgtgtc ggggcagctg ccggagagct tcgtcgcgca ggccgagctg gcctggcgca
     2221 acgtgcacac catcctggcg caggccggca tgggggtcga ggacatcgtc aagctcaccc
     2281 agtacctggt gcgccgcgag gacctggccg attatcgcga gatccgctcg cgcaacctgc
     2341 gcgagcatcg cccggcctcg atgctgtcgt tcgtggatca gctggtgtgg cccaacatgc
     2401 tgatcgagct ggaggtggtg gcggcacagt agccggctct ctctcccgca gagaaaacgg
     2461 tcactgatac cgcagcgcct cgatcggctt gaggtcggcc gccttgcgcg cgggatagaa
     2521 gccgaagaac acgcccacgg cggcggccac caggaaggcg agcccgatcg ccaccgcggt
     2581 gaccaccacc gtcatctgcg tcacccggct caccagcaac gccgccgcca cgcccaggat
     2641 cacgccgatc aggcagccga tcaccgacac gatcagcgac tccagcagga actgcagcag
     2701 gatgtcgcgg cggcgcgcgc cgatggccat gcggatgccg atctcgcggg tgcgctcggt
     2761 caccgacacc agcatgatgt tcatgatgcc gatgccgccc accaccagcg acaccgaggc
     2821 caccgcgccg agcatgatcg acatgacacg cgtggtctgc gcctgcacgt cggccaccgc
     2881 gctcaggttg cggatggtga agtcggcctc gccatcgggc gggatgcggt ggcgggcgcg
     2941 cagcagcgcg tcgatcgcgc gctcggcgcg cggcatggcc tcctcggaca gcgccttgat
     3001 catcatgaag cgcaccgtgc ccgggaacgg cgagccgtac agcttgcgct gggcggtggt
     3061 gagcgggacc agcaccgtat cgtcctgatc gcgcccgtcg aagctctgtc ccttggaggc
     3121 cagcacgccc agcaccaggt aggggctgtt gcggatacgc acggtcttgc caaccgggtc
     3181 ctcgtcgccg aacacctgct tcgccacggt ctgccccagc agggccacgc gcgccaccgc
     3241 gcgcacatcg gcctccgtga acggctgccc cgccacgagg cgccattccc gcacctcgaa
     3301 gtaacttggc gtaacgccca tcacctgggt attccagttg gccgcgccgt acacgagctg
     3361 tgcgttgccg aactgggtgg gggcgactgc cgcgacgctg gcgagctcgc cgatcgcgtc
     3421 ggcgtcggcc agcgtcaggg tgggcgcggt gccgctgccg gtgcgcgccc cgccggcgct
     3481 ggtgccgccg gagagcacga tgaacaggtt gctgcccatg gtcgccaccg cttcccggac
     3541 cagcgtctgc gcgccctggc cgatggccat catcagcacc accgcgccca cgccgatgac
     3601 caggccgagc atggtgagga aggtgcgcag ccggttggcg cccatcgcgc tccaggcttc
     3661 gcgcagcatg cccgcgatca tagtgaggcc tggagcgcgt gggaggcggg ggccgcgccg
     3721 cgtatctcgt cgctgtcgac cacgccgtcc acgaagtgga tcacgcgctt gccgaagcgc
     3781 gcgacgtccg cttcgtgcgt gaccagcacc acggtggtgc cgtgctcggc gttgaggccg
     3841 gcgattagcc gcatgatgtc gtggctggtt gcgctgtcga ggtttccggt gggctcgtcg
     3901 gcgagcacca ggcgcggcga gttgatcagg gcgcgggcga tggccacgcg ctgctgctgg
     3961 ccgcccgaga tgcggctggg cagataggcg gcgtagcgct ccaggcccac gcgcgccagc
     4021 agctcatgcg cgcgctcctc gcggctgcgc accgggacgc ccgcgtacag cagaggcagc
     4081 gcgacgttgt ccagcagcgt ggcccgcggc agcaggttga agccctggaa cacgaagccg
     4141 atgaatcggt tgcgcagccg cgccagcgcg tcgcccgaga ggcgcgaggt gtcgttgccc
     4201 tgcaggagat agcgcccgct gctcgccacg tccaagcagc cgaggatgtt catgaaggtg
     4261 gacttgcccg agcccgacgg gcccatcacc gcgacgaact cgcccggttc gatcctcagg
     4321 ctgacgtcgc gcagcaccgg ctgcgggccc gcgtcggtct cgtactgctt gtgcaggtgc
     4381 tcgatcagga tcagcggcgc gctcgccgcg gcgcgatcca tcagaagccc cgaatgcgga
     4441 acggctggcc ggtgccggac gcgggcttgc gctcgccggt caactcttcg accactaccc
     4501 ggtcgccggc cttcagctcg ccctcggtca ccacggtcga gcggccgtcg gagatgccgg
     4561 tgcgcagcg
//
LOCUS       DASPGE010000360         9874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1146135, whole genome shotgun sequence.
ACCESSION   DASPGE010000360 DASPGE010000000
VERSION     DASPGE010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9874
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146135"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VD791_11650"
     CDS             <1..899
                     /locus_tag="VD791_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HXV08678.1"
                     /translation="DCLLHDMGQHHPECPDRLRAIEDQLIASGLAGYLDEYDAPLAAR
                     EHLARVHAPEYLDALEGAAPERGLVHLDPDTAMSPHTLQAAARAAGAAVMATDLVLEG
                     TARNAFCCVRPPGHHAERRRAMGFCFYNNVAVAAAHALERHGLERVAMVDFDVHHGNG
                     TEDIFRDDGRVLMVSTFQHPFYPYSGVDGRSERMVNIPLAAYSDGAKFRAAVEAWWLP
                     ALEQFRPQMLFVSAGFDAHREDDMASLSLVEGDYTWVTQQIKAVAERHARGRIVSMLE
                     GGYNLSALGRSAVAHLRVLADL"
     gene            complement(959..1336)
                     /locus_tag="VD791_11655"
     CDS             complement(959..1336)
                     /locus_tag="VD791_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HXV08679.1"
                     /translation="MKLHQTRAQGRNLFTGYGPGYVSVSGNRWESSVIVLPDRTERWD
                     VAGFDALTEDTFTRLAELPIQILLLGSGARLRFPHPRLTQALIRAGIGLEVMDTQAAC
                     RTYNILLEEGRRVAAALLIGAAD"
     gene            1441..2643
                     /gene="alaC"
                     /locus_tag="VD791_11660"
     CDS             1441..2643
                     /gene="alaC"
                     /locus_tag="VD791_11660"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HXV08680.1"
                     /translation="MEQFPRIKRLPPYVFNITAELKAAARKRGEDVIDFGMGNPDQPT
                     PRHIVDKLIETVQRADTHRYSVSKGIFRLRKAICNWYKGRYDVELDPDREAIVTIGSK
                     EGIAHLALATLDDGDIVLVPNPSYPIHIYGPVISGADIRHVRMLPGVDFFEELEQALK
                     DSYPKPKMLILNFPSNPTSQCVELPFFERIVALAREYGIYVVHDLAYADIVFDGYKAP
                     SILQVPGAKEVAVEFFTLSKSYNMAGWRVGFMVGNPDLVSALARIKSYHDYGTFTPIQ
                     VAAIVALEGPQACVEQVRMTYQKRRDVLCAGLNGLGWRVQVPKASMYVWAPIPAAYRA
                     MGSLEFAKKLLGDARVAVAPGIGFGQYGDDHVRFAMIENEARTRQAIRGIRDMFRRDG
                     LPQLAAAG"
     gene            2647..3090
                     /locus_tag="VD791_11665"
     CDS             2647..3090
                     /locus_tag="VD791_11665"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV08681.1"
                     /translation="MLQIIRITDDARRVVERDWLARAEGVHRQLRPSLPAGYVARMQR
                     IFEAGGEMCVAVEEGQVVGVAVFRSFDNTHHGKRFYVDDLVTDEARRSSGVGHALLAF
                     LEGLARARGGHSIDLDSGTHRTRAHRFYFREGFVIPSFVFRKDLP"
     gene            3087..4400
                     /locus_tag="VD791_11670"
     CDS             3087..4400
                     /locus_tag="VD791_11670"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HXV08682.1"
                     /translation="MKPIHVGLLGIGTVGGGTFRVLTRNQSEITRRAGRGIQITVVAD
                     KDLAKARALVGDAAKVTDDAFAVVRDRDIDIVVELIGGYTVAKDLILAAIDNGKHVVT
                     ANKALLATHGNEIFAAAQRKGVMVAFEAAVAGGIPIIKALREGLTANRIEWIAGIING
                     TSNFILSEMRDRGASFEQALQEAQRLGYAEADPTFDIEGIDAAHKLTILSAISFGIPM
                     QLNRAYTEGISKLTRHDIRYAEELGYRIKLLGITRRTAQGIELRVHPTLIPARRLIAN
                     VEGVMNAILIKGDAVGQTLYYGAGAGSEPTASAVVADLVDVTRLHTADPESRVPHLAF
                     RPDQLSDTPILPMDEVETAYYLRLRVLDRPGVLADITRILADLGISIDAMVQKEPGEG
                     EEQVDIIMLTHRTLEKHVIAAIARIEGLPANLAEVTRIRVEELAA"
     gene            4425..5837
                     /gene="thrC"
                     /locus_tag="VD791_11675"
     CDS             4425..5837
                     /gene="thrC"
                     /locus_tag="VD791_11675"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXV08683.1"
                     /translation="MRYLSTRGDMPSRRFTEILLEGLAPDGGLVVPESYPRVSLEELA
                     VWRRLSYAELAGEILRRYADDIPGADLRGLVERTYTASAFRTADITPVKALEPGLHIL
                     ELSNGPTLAFKDLAMQLLGNLFEYVLGRSAQQLNILGATSGDTGSAAEYAMRGKRGIR
                     VFMLSPYGRMSPFQTAQMYSLQDANIFNIGVRGVFDDCQDIVKAVSNDAGFKARYRIG
                     TVNSINWARVVPQAVYYFKAYLAVVRDMGEPVSFAVPSGNFGNIFAGHIARQMGLPIQ
                     RLILATNENDVLDEFFRTGRYRPRGAAEVQQTSSPSMDISKASNFERFVYDLVGRNSR
                     VLVQLWQSLERAGGFDLTGTEYLGRLPQFGFVSGTSTHADRMNTIRQVYREYGVMIDT
                     HTADGLKVGLEHRETGVPLVCLETALPAKFAASIREALGRDPDLPPGYDGLERLPQRV
                     EVMDADAAAVKAFIARHCEG"
     gene            5841..6467
                     /locus_tag="VD791_11680"
     CDS             5841..6467
                     /locus_tag="VD791_11680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV08684.1"
                     /translation="MAEGFDDARAFWDQRFAAPEYIFGKAPNRFLVSQAQWLRPGMRV
                     LDVAAGEGRNAVWMAGQGCEVLGIDISPLAVQKAERLAAEAGVRVAFEVADVRQWRWP
                     PEAFDAVVSIFIQFAAPGERSAVFEGMKRTLRPGGVLIMQGYTPKQIEYKTGGPPQAG
                     HMYTEALLRAAFGDLEILHLREHEDVLSEGVKHVGRSALIDLVARRPT"
     gene            complement(6561..7328)
                     /locus_tag="VD791_11685"
     CDS             complement(6561..7328)
                     /locus_tag="VD791_11685"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXV08685.1"
                     /translation="MKLFDLSGKVAVVTGGNGGIGLGMARGLAAAGAAIVVAGRDAKK
                     NTDALAALSAAGARAIAASVDVRRKDSCQAMVDAATKAFGRLDILVNNAGINIRKQPQ
                     EYSVEDWQTVIDTNLSSAFLCCQAAYPAMLRAGGGKIVNIGSMMSIFGAPFTAAYAAS
                     KGGLVQLTRALATAWAKDNIQVNAVLPGWIDTDLTRKAREQVSGLHERVVARTPAARW
                     GTPEDLAGIAVFLASPAADFVTGTAIPIDGGYSVQGV"
     gene            complement(7408..7914)
                     /locus_tag="VD791_11690"
     CDS             complement(7408..7914)
                     /locus_tag="VD791_11690"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV08686.1"
                     /translation="MDIGPVRTLAIGLTALGLSLLAAAPAAGGEIAPVRLPASEIPWP
                     ETTARVEGTGMRAGLQVLVVAGDAKSNGLYTMMFKLPANRRVPPHSHPDVRSCFVLSG
                     TFYFAYGDTWDDTLLQALPAGSHYTEPAGMNHFAASRDEDVIAECTAVGPTGTTFVNP
                     ADDPRRQQ"
     gene            complement(7934..8785)
                     /locus_tag="VD791_11695"
     CDS             complement(7934..8785)
                     /locus_tag="VD791_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXV08687.1"
                     /translation="MDSASQPALAAGTVQLGDRTVHRMGFGAMRLCGPSVWGPPKDRA
                     AAHAVLRRAVELGIDFIDTADSYGPEVDETLIAEALHPYPDGLIVGTKGGLLRPSPPR
                     WDTDGRPEHLRQALEGSLKRLKLDCIDLYQLHAPDSRAPFADSVGALADAQRAGKIRH
                     IGLSNVSVKQLEQARRIAPIVSVQNRYNLEDRGSEDVLAACERLGIAFLPWFPLGAGA
                     ALRSANVKRVAARAGASPAQVLLAWLLTKSAAMLPIPGTASLAHLEENTRAAMLKLSA
                     DDFAALA"
     gene            8861..9631
                     /locus_tag="VD791_11700"
     CDS             8861..9631
                     /locus_tag="VD791_11700"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HXV08688.1"
                     /translation="MNEHLIEHSRRWVDEARRVVVLTGAGISTDSGIPDFRGPQGVWT
                     RNPAAEKLSTLQHYLADPEVRKTAWRTRLEHPAWHARPNPGHMALVALERRGKLHALI
                     TQNIDELHQLAGNAPERVIEVHGTMRKVTCWGCGWIGPMQAALERVRAGEEDPPCSAC
                     GGVLKSATISFGQQLVPEVIDRALRSAAEADVFVAVGTSLQVYPVAGALPAARAAGAK
                     VVIVNAEPTAMDALAHAALAGSISAILPRVLGSDRVHA"
BASE COUNT         1562 a         3353 c         3423 g         1536 t
ORIGIN      
        1 aggactgcct gctgcacgac atgggacagc atcatccgga gtgcccggat cggctgcgcg
       61 ccatcgaaga ccagctgatc gcctcgggcc tggccggata cctggacgag tacgacgccc
      121 cgctcgccgc ccgtgagcac ctcgcccggg tgcacgcgcc ggagtatctc gatgccctgg
      181 aaggcgcggc gccggagcgc ggcctggtgc acctcgaccc cgacaccgcg atgagcccgc
      241 acacgctgca ggcggctgcg cgcgccgccg gtgcagcggt gatggcaacc gatctggtgc
      301 tcgagggcac ggcgcgcaac gccttctgct gcgtgcgtcc gcccggccat catgccgagc
      361 gccggcgcgc catgggtttt tgcttctaca acaacgtcgc ggtggccgcc gcccacgcgc
      421 tcgagcggca cgggctggag cgcgtcgcga tggtggattt cgacgtgcac cacggcaacg
      481 gcaccgagga catcttccgc gacgacggcc gggtgctgat ggtgtccaca tttcagcacc
      541 cgttctaccc gtacagcggg gtggacgggc gctcggagcg tatggtcaac atcccgctcg
      601 cggcctacag cgacggggcg aaattccgtg ccgccgtcga ggcgtggtgg ctccctgcgc
      661 tggagcagtt ccggccgcag atgctgttcg tctcggccgg tttcgacgcc caccgcgagg
      721 acgacatggc ctccctgtcg ctggtggaag gcgactatac ctgggtgacg cagcagatca
      781 aggcggtggc cgagcgccac gcccgaggac ggatcgtctc gatgctggaa ggcggctaca
      841 acctgagcgc gctgggtcgc agcgcggtgg cgcacctgcg ggtgctggcc gatctgtgag
      901 cgcaggcgct cgatgcgctg ctctcgctga tagtgcgacg agcgctgcac gcacatgatc
      961 agtctgccgc cccgatcaac agcgccgcgg ccacgcgccg tccctcttcc agcaggatgt
     1021 tgtacgtgcg gcaggcggct tgcgtgtcca tcacctccaa gccgatgccg gctctgatca
     1081 acgcctgcgt cagccgcggg tgcgggaagc gcaatcgagc accggagccg agcagcagga
     1141 tctggatcgg cagctcggcg aggcgggtga aagtgtcctc ggtcagggcg tcgaagccgg
     1201 cgacgtccca gcgttcggtg cggtccggca gcacgatcac gctgctttcc caacggttgc
     1261 cgctgacgct gacgtagccg ggcccgtagc cggtgaacag gttgcgcccc tgggcgcgcg
     1321 tctggtgcag tttcattgca tgcaataaat ctcgtcgagc gggcccgccc gcattgcccg
     1381 ggcggccgcg cttggttaaa attctagttt tcccatcagt ctgttaggtc cgcgccttcc
     1441 atggagcagt tcccccgcat caagcgcctg ccgccgtacg tcttcaacat caccgccgag
     1501 ttgaaggccg ctgcccgcaa gcgcggcgag gacgtcatcg atttcggcat gggcaatccc
     1561 gaccagccaa cgccgcgcca catcgtggac aagctgatcg aaaccgtcca gcgcgccgat
     1621 acgcatcggt actcggtctc caagggcatc tttcgcctgc gcaaggcgat ctgcaactgg
     1681 tacaaggggc gctacgatgt ggagctcgac cccgaccgcg aggcgatcgt caccatcggc
     1741 tccaaggaag ggatcgcgca cctggcgctc gccaccctgg acgacgggga catcgtgctg
     1801 gtgcccaatc ccagctaccc gatccacatc tacggcccgg tgatctcggg cgccgacatc
     1861 cgccacgtgc gcatgctgcc gggggtggat ttcttcgagg agctggaaca ggccctcaag
     1921 gacagctacc cgaagcccaa gatgctgatc ctgaacttcc ccagcaaccc caccagccag
     1981 tgcgtggagc tgccgttctt cgagcgcatc gtggcgctcg ccagggagta cgggatctac
     2041 gtggtgcacg atctcgccta cgccgacatc gtgttcgacg gctacaaggc gccctccatc
     2101 ctgcaggtgc ccggcgcgaa ggaggtggcg gtggagttct tcacgctgtc caagagctac
     2161 aacatggccg gatggcgcgt cggcttcatg gtcggcaatc ccgatctggt ctcggcgctc
     2221 gcgcgcatca agagctacca cgactacggc accttcaccc ccatccaggt ggccgccatc
     2281 gtcgcgctgg agggaccgca ggcctgcgtc gagcaggtgc gcatgaccta tcagaaacgc
     2341 cgcgatgtgc tgtgcgcggg tctgaacggc ctgggctggc gcgtccaggt gccgaaggcg
     2401 agcatgtacg tctgggcgcc gattcccgcc gcctaccgcg ccatgggctc gctcgagttc
     2461 gccaagaagc tgctcggcga cgccagggtc gcagtggccc cgggcatcgg cttcggtcaa
     2521 tacggcgacg atcatgtacg cttcgcgatg atcgagaacg aagcccgcac ccggcaggca
     2581 atccgcggta tccgcgacat gttccgccgg gacgggctgc cgcagctcgc ggcggcgggg
     2641 taggcggtgc tgcagatcat ccgcataacc gacgacgcgc gccgggtggt ggagcgcgac
     2701 tggctcgcgc gtgccgaggg tgtgcaccgg cagttgcggc cgagtctgcc cgccggctac
     2761 gtggcgcgca tgcagcgcat cttcgaggcc ggcggcgaga tgtgcgtcgc ggtcgaggag
     2821 ggtcaagtgg tcggggtcgc ggtgttccgc tccttcgaca acacccacca cggcaagcgc
     2881 ttctacgtgg acgacctggt gaccgacgaa gcgcggcgct cgagcggggt cgggcacgcc
     2941 ctgctcgcgt tcctggaagg gctggcgcgc gcccgcggcg gccacagcat cgatctcgac
     3001 tcgggcaccc atcgcacgcg cgcgcaccgc ttctacttcc gggagggctt cgtgattccc
     3061 tccttcgtgt tccggaagga tctgccatga agccgatcca cgtcggcctg ctcggcatcg
     3121 gcaccgtcgg cggcggcacc ttccgcgtgc tcacgcgcaa ccagagcgag atcacccggc
     3181 gcgccggccg cggcatccag atcaccgtgg tggccgacaa ggatctcgcg aaggcgcgcg
     3241 cgctggtcgg cgatgccgcg aaggtgaccg acgacgcctt cgcggtggtg cgcgatcgcg
     3301 acatcgacat cgtggtggag ctgatcggcg gctacaccgt ggccaaggat ctgatcctgg
     3361 ccgcgatcga caacggcaag cacgtggtga ccgccaacaa ggcgctgctc gccacgcacg
     3421 gcaacgagat cttcgccgcc gcccagcgca agggcgtgat ggtcgcgttc gaggccgcgg
     3481 tggccggcgg aatcccgatc atcaaggcgc tgcgcgaggg gctcacggcg aaccgcatcg
     3541 agtggatcgc cggcatcatc aacggcacca gcaacttcat tctctccgag atgcgcgacc
     3601 ggggagcgag cttcgagcag gcgctgcagg aggcgcagcg cctgggttac gccgaggccg
     3661 atccgacttt cgacatcgaa ggcatcgacg ccgcgcacaa gctgaccatt ctctccgcca
     3721 tcagcttcgg cattccgatg cagttgaatc gcgcctacac cgaaggcatc tccaaactta
     3781 cccgacacga catccgctac gccgaggagc tgggctatcg catcaagctg ctgggcatca
     3841 cgcgccgcac ggcccagggc atcgagttgc gggtgcaccc gaccttgatt ccggcgcggc
     3901 ggctgatcgc caacgtggaa ggggtgatga acgcgatcct gatcaaaggc gatgcggtcg
     3961 ggcagacgct gtactacggc gcgggcgcgg gatccgagcc caccgcctcg gccgtggtgg
     4021 cggacctggt ggacgtgacg cgcctgcaca ccgccgaccc ggagagccgc gtgccgcatc
     4081 tcgccttccg ccccgaccag ttgtcggaca cgccgatcct gccgatggac gaggtggaga
     4141 ccgcctatta cctgcggctg cgggtgctgg atcggcccgg agtgctggct gacatcaccc
     4201 gcatcctggc cgatctcggc atctccatcg acgccatggt gcagaaagag ccgggggagg
     4261 gcgaggagca ggtggacatc atcatgctca cgcaccggac gctggagaag cacgtcatcg
     4321 cggccatcgc ccgcatcgag ggactgccgg ccaacctggc tgaagtcacc cgcatccggg
     4381 tggaggagct ggccgcgtga gcggtctcgc ctgaccgcgg caccatgcgt tacctcagca
     4441 cccgcggcga catgccgtcg cgccgcttca ccgagatcct gctggagggc ctggcgcccg
     4501 acggcggact ggtggtgccc gagagctacc cgcgcgtgag cctcgaggag ctggccgttt
     4561 ggcgccgact gtcctacgcc gagctggccg gcgagatcct gcgccgctac gcggacgaca
     4621 tcccgggagc ggatctgcgc ggcctggtgg agcgcaccta caccgcgagc gcgttccgca
     4681 ccgcggacat cacgccggtc aaggcgctgg agcccgggct gcacatcctg gaactgtcca
     4741 acggtcccac actcgccttc aaggacctgg cgatgcagct gctgggcaac ctgttcgaat
     4801 acgtgctcgg caggagcgcg cagcagctca acatcctggg cgccacctcg ggcgacaccg
     4861 gctcggccgc ggaatacgcc atgcgcggca agcgcggcat ccgtgtattc atgctctcgc
     4921 cgtacgggcg catgagtccg ttccagacgg cgcagatgta ctcgctgcag gacgccaata
     4981 tcttcaacat tggcgtgcgc ggggtgttcg acgactgcca ggacatcgtc aaggcggtca
     5041 gcaacgacgc cggcttcaag gcccgctacc gcatcggcac ggtcaactcg atcaactggg
     5101 cgcgcgtggt accgcaagcg gtctactact tcaaggcgta cctggcggtg gtgcgagaca
     5161 tgggcgagcc ggtctccttc gccgttccct ccggcaattt cggcaacatc ttcgccgggc
     5221 acatcgcgcg ccagatgggc ttgccgatcc aacgtctgat cctggccacc aacgagaacg
     5281 acgtgctgga cgagttcttc cgcaccgggc gctaccggcc gcgtggcgcc gcggaagtgc
     5341 agcagacctc cagtccctcc atggacatct ccaaggcgtc gaacttcgag cgcttcgtct
     5401 acgacctcgt cggtcgcaac agccgcgtgc tcgtgcagct gtggcagagc ctggagcgcg
     5461 caggcggctt cgacctcacg ggcaccgagt acctgggccg gttgccgcag ttcggcttcg
     5521 tctccggcac gagcacccat gccgaccgca tgaacaccat ccggcaggtc tatcgcgagt
     5581 acggcgtgat gatcgatacg cacacggcgg acgggctcaa agtgggcctg gagcatcgcg
     5641 agacgggcgt gccgctggtg tgcctggaga ccgcgttgcc ggcgaagttc gccgcctcga
     5701 ttcgcgaggc gctgggccgc gacccggact tgccgcccgg ttacgacggg ctcgagcggc
     5761 tgccgcaacg ggtcgaggtg atggatgcgg atgccgcggc ggtgaaggcg ttcatcgccc
     5821 ggcactgcga gggctgaagc atggccgagg gtttcgacga cgcgcgcgcc ttctgggacc
     5881 agcgcttcgc cgcgcccgaa tacatcttcg gcaaggcgcc caaccgtttc ctggtgtccc
     5941 aggcgcagtg gctgcgtccc ggcatgcgcg tgctcgacgt cgccgccggg gaggggcgca
     6001 acgccgtgtg gatggccggg caggggtgcg aggtgctcgg catcgacatc tcgccgctcg
     6061 ccgtccagaa agccgagcgc ctggccgccg aggccggtgt ccgggtcgcg ttcgaggtcg
     6121 ccgatgtgcg ccagtggcgc tggcccccgg aggctttcga cgccgtggtg agcatcttca
     6181 ttcagttcgc cgcaccgggc gaacggtccg cggtgttcga gggcatgaag cgcacgctgc
     6241 gtccgggcgg cgtgctgatc atgcagggct acacacccaa gcagatcgag tacaagaccg
     6301 gcggcccccc gcaggcgggg cacatgtaca ccgaagcgct gctgcgcgcg gcgttcggcg
     6361 acctcgagat cctgcacctg cgcgagcacg aggacgtgct cagcgagggc gtcaagcacg
     6421 tcgggcgttc ggcgctgatc gatctggtgg cgcgtagacc aacgtagccc ggatggaggc
     6481 cgtcaggccg gactccgggg ccccggattc ggcttcgctg catccaggct acggagtggc
     6541 gcgcagtcac cttccgtctc tcagacgccc tggaccgaat agccgccgtc gatcgggatc
     6601 gcggtgccgg tgacgaagtc cgcggcaggg ctcgccagga acacggcgat gcccgccaag
     6661 tcctccggcg tcccccagcg cgccgccggg gtgcgcgcca cgacccgctc gtgcagcccc
     6721 gacacctgct cgcgcgcctt gcgcgtcaag tccgtgtcga tccatcccgg cagcacggcg
     6781 ttgacttgaa tgttgtcctt ggcccaggcg gtggcgagcg cgcgcgtgag ctggaccagc
     6841 cctcccttgc tggcggcgta cgccgccgtg aagggcgcac cgaagatcga catcatggag
     6901 ccgatgttga cgatcttgcc gccgccggcc cgcagcatcg cgggatacgc cgcctggcag
     6961 cagagaaaag cgctgctcaa attggtgtcg atcaccgtct gccagtcctc gaccgagtac
     7021 tcctgcggct gcttgcggat gttgatgccg gcgttgttca ccaggatatc cagccggccg
     7081 aacgccttgg tcgcggcgtc gaccatcgcc tggcacgagt ccttgcgccg cacgtccacg
     7141 gacgcggcga tcgcacgcgc gcccgcggcg ctcaacgccg ccagcgcatc ggtgttcttc
     7201 tttgcgtcgc gaccggccac cacgatcgcc gccccggccg cagcgagccc gcgcgccatg
     7261 cccaggccga tgccgccgtt gccgccggtc accacggcca ccttgccgct caggtcgaac
     7321 agtttcatgc gccagcgctc cttttgcgtg tgagtgggct tgccggacgt ggagatcagg
     7381 ccgctgcgtg atcggccgcc cgcgccgtca ctgctggcgg cgcggatcgt cggcggggtt
     7441 gacgaaagtg gtgccggtgg gaccgactgc cgtacattcg gcgatgacgt cctcgtcgcg
     7501 cgaggcggcg aaatgattca tccccgccgg ctcggtgtag tggctgccag ccggcagagc
     7561 ctgcagcagc gtgtcgtccc aggtgtcgcc ataggcgaag tagaacgtcc ccgacagcac
     7621 gaagcaagag cgcacatcgg ggtgtgagtg tggcggcacg cggcggttgg ccggaagctt
     7681 gaacatcatg gtgtacaggc cgttgctctt cgcgtccccg gccaccacca ggacctgcag
     7741 gccagcgcgc atgcccgtgc cctcgacgcg tgcggtggtt tccggccatg ggatctcgga
     7801 ggccggcaag cgaaccggcg cgatctcgcc gccggccgcg ggtgccgcgg cgagcagcga
     7861 caggcccagc gcggtgagcc caattgcaag tgtgcgaacg ggtccgatgt ccattgctat
     7921 cgtcctcctc acgtcaggct agcgcagcga aatcgtcggc ggaaagtttc agcatcgccg
     7981 cgcgcgtgtt ctcctcgagg tgtgcgagcg acgccgtgcc ggggatcggc agcatcgccg
     8041 ccgattttgt gagcaaccag gcgagcagca cctgggccgg cgaggcgccc gcgcgggcgg
     8101 cgacgcgctt gacgttggcc gatcgaagcg ccgcgcccgc gccgaggggg aaccagggca
     8161 ggaaagcaat gccgagacgc tcgcacgcgg cgagcacgtc ctcggatccc cggtcttcga
     8221 ggttgtagcg gttctgcacc gagacgatgg gggcgatacg gcgtgcctgc tcgagctgct
     8281 tcacgctcac gttggacagg ccgatgtgcc tgatcttgcc ggcgcgctgg gcgtccgcca
     8341 gggcgccgac ggagtcggcg aacggcgctc tggaatcggg agcgtgcagc tggtacaaat
     8401 cgatgcagtc gagtttcagg cgcttgaggc tgccctcgag cgcctggcgc aggtgctcgg
     8461 gccggccgtc ggtgtcccag cgcggggggc tggggcgcag gaggccgccc ttggtgccga
     8521 cgatcaagcc atcggggtag gggtgcagcg cttcggcgat cagcgtctca tcgacctcgg
     8581 gaccgtagct gtccgcggtg tcgatgaaat cgatccccag ctccaccgct cggcgcaaca
     8641 cggcgtgcgc ggcggcacgg tccttcggcg ggccccagac gctcggtcca cacaagcgca
     8701 tcgcgccgaa acccatgcga tggacggtgc ggtcgccgag ctggacggtg cccgcggcga
     8761 gcgcgggctg cgaggccgaa tccatgcggg cagtttagac tatccaccga tgacggcgag
     8821 tccatgggtg gtgaacgagg ggggcggtga acgaggggcg gtgaacgagc acctgatcga
     8881 acactcgcgg cggtgggtgg atgaagcgcg gcgcgtggtg gtgctgaccg gcgccggcat
     8941 ctccaccgac tcggggattc cggatttccg cgggccgcag ggcgtgtgga cccgcaaccc
     9001 ggcggccgag aagctctcca ccctgcagca ttacctcgcc gacccggagg tgcgcaagac
     9061 cgcgtggcgc acgcgcctcg agcaccccgc ctggcacgcg cggccgaatc ccgggcacat
     9121 ggcgctggtg gcgctcgagc ggcgcggcaa gctgcacgcg ctgatcacgc agaacatcga
     9181 cgagctgcac cagctcgccg ggaacgcgcc ggagcgcgtg atcgaagtgc acggaacgat
     9241 gcgcaaggtg acctgctggg gctgcggctg gatcggtccg atgcaggcgg cgctcgagcg
     9301 cgtgcgcgca ggcgaagagg acccgccttg cagcgcctgc ggcggcgtgc tgaagagcgc
     9361 caccatctcc ttcggccagc agctcgtgcc cgaggtcatc gaccgcgcgc tgcgctcggc
     9421 cgcggaagcg gacgtgttcg tggcggtcgg caccagcctt caggtgtacc cggtcgccgg
     9481 cgccctgcct gccgcccgcg cggccggggc gaaggtggtc atcgtcaacg ccgagccgac
     9541 cgccatggat gcgctcgcgc acgccgcgct cgcgggctcg atcagcgcga tcctgccgcg
     9601 cgtgctcggc tcggaccggg tccacgcgta gcgcgtctgt atcagcgtag cgagaagaat
     9661 gtggcggttg cgtaccaccc cgtcacgctt cgcgtgccac cccgccgagg gcgcccttaa
     9721 ggcgcgactt aaaaaacatc tccccgcctc tcaggaggcg gggtgccggc ggggcggtgt
     9781 ggtgtgtgct gcccttaccg agccagcgcg gcgggcagct cgtccaggcg ggtgaggacc
     9841 gaggcgggcg cgaagcccag ctcgtccggc tggg
//
LOCUS       DASPGE010000361         3124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_561226, whole genome shotgun sequence.
ACCESSION   DASPGE010000361 DASPGE010000000
VERSION     DASPGE010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561226"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="VD791_11705"
     CDS             complement(<1..1349)
                     /locus_tag="VD791_11705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXV08689.1"
                     /translation="MAAFRTRFWICLILTVPVLLLSPPGEHAVGPIGALAFPGSQWLL
                     LALGSVVYFYGGWPFLSGLAGELAKRRPGMMTLIALAISVAYLYSAAVVLGLRGNLLF
                     WELATLIDIMLLGHWIEMKSVLGASGALRALVELLPKTAHRVAADGALMDVPIESLQP
                     GDRVLVKPGERVPTDGVIVDGRTSVDESMLTGESKPVPRGPGERLIGGAVNAEGAVTV
                     QVDRTGAQTYLAQVVDMVSKAQASRSRTQDLANRAALWLTYIAVGAGLATLLVWIALG
                     TPLSFAVERMVAVMVISCPHALGLAVPLVVAVSTTLAARQGLLIRDRAAFERARGLNA
                     VVFDKTGTLTEGRFGVTDVVPFAQTDARELIAVAAALESQSEHPIAKGIVEHAQALGV
                     EYRRATDFRNITGQGAVARLDGQPIAVVSPGYVQQRGLTVDDPRLERLQAEAKTLVFV
                     "
     gene            1484..>3124
                     /locus_tag="VD791_11710"
     CDS             1484..>3124
                     /locus_tag="VD791_11710"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXV08690.1"
                     /translation="MSSADTLTPPAATRKPAREWTFPVEGMTCASCVARVEKALRRVP
                     GVREANVNLATESATVAAGADTPREALVKAVEEAGYGVPREEVALGIEGMTCASCVGR
                     VEKALHAVPGVLNASVNLATERAQVQTAGGVPVDALLTAVEKAGYRGTPASTAPAASA
                     AVAKQRYDWWPVALAALLSLPLMIPMLTAPFGVHWMLPGWMQWLLATPAQFWLGARFY
                     RAGWKALRAGTGNMDLLVAIGTSAGYALSVYHLLAAGQEQMGHLYFEASAVVITLVLL
                     GKWLETRAKRQTTEAIRALQALRPERARVRRDGREREVPAEAVRAGDEVIVRPGERIP
                     VDGEVLEGRSHADESLITGESLPVAKAPGDRVTGGSVNAEGLLAVRTTAVGAETTLAR
                     IIRLVESAQAHKAPIQRLVDRVSAVFVPVVIGIAVAALLGWGSATGDWTQATLNAVAV
                     LVIACPCALGLATPTAILAGTGVAARAGILIKDAEALEIAHGVRTVAFDKTGTLTEGR
                     PGLVALATSPGVAEGDALAWAAALQKGSEHPLARAVLAA"
BASE COUNT          467 a         1067 c         1146 g          444 t
ORIGIN      
        1 acgaacacca gggtcttcgc ttccgcctgc aagcgctcca ggcgcggatc atcgactgtg
       61 agcccgcgct gctggacgta tccggggctc accacggcga tgggttgccc gtcgagccgg
      121 gcgactgcgc cctggccggt gatgttgcgg aagtccgtgg cgcgccgata ctcgacaccc
      181 agcgcttgcg cgtgctccac gatgcctttc gcgatgggat gctccgactg cgactcgagc
      241 gcggccgcca ctgcgatcag ttcgcgcgcg tcggtctgcg cgaagggaac cacgtcggtc
      301 acgccgaagc gcccctcggt cagcgtgccg gttttgtcga acacgaccgc gttcagcccg
      361 cgggcgcgct cgaaggcggc gcgatcgcgg atcagcaggc cctgccgtgc ggccagggtg
      421 gtgctgaccg cgaccaccag cggcaccgcc aggcccagcg cgtgcgggca ggagatgacc
      481 atcaccgcga ccatgcgctc cacggcgaag gacagaggcg tgccgagcgc gatccatacc
      541 agcagcgtgg cgagccctgc cccgacggcg atgtaggtca gccacagggc ggcccgattc
      601 gccagatcct gcgtgcgcga gcgcgacgcc tgcgccttgc tcaccatgtc gaccacctgc
      661 gcgaggtagg tctgtgcgcc ggtgcgatcc acctgcacgg tgaccgcccc ctcggcgttg
      721 acggcgccgc cgatgagccg ctcgccgggg ccgcgcggca ccggcttgga ctctccggtg
      781 agcatggatt cgtcgacgct ggtcctgccg tccacgatga cgccgtcggt gggcacacgc
      841 tccccgggct tgaccaacac ccgatcgccc ggctgaagcg actcgatcgg tacgtccatc
      901 agcgcgccat cggcggcgac ccggtgcgcg gtcttgggca acagctccac cagcgcgcgc
      961 agtgcgcccg atgcgcccag caccgacttc atctcgatcc agtggccgag cagcatgatg
     1021 tcgatcagcg tggccagctc ccagaacaac aggttgcccc gcagccccag caccaccgcc
     1081 gcggaataca gataagcgac gctaatcgca agcgcgatca gcgtcatcat ccccggccgg
     1141 cgcttggcca gctcgccggc caggccgctc agaaacggcc agccgccgta gaagtagacg
     1201 acgctgccca gcgcgagcag cagccactgc gaccccggga aggcgagcgc gccgatcggc
     1261 ccgaccgcgt gctcgcccgg cggggacagc agcagtaccg ggacggtgag gatcagacag
     1321 atccagaacc tcgtccgaaa cgccgccacc atttgggcgt ggtcgtggtg cgcgtgggga
     1381 cccggccgcg ctgaggcgga cacgcgcgct tcgggttggg cgtggtggtg ggctattgga
     1441 aactccaaac ttgccgcaag ctatagggac cgaggatatc ccagtgagca gcgccgatac
     1501 tctgacgccc ccggcggcaa cccgcaagcc cgcccgtgag tggacgtttc ccgtggaggg
     1561 catgacctgc gcttcgtgcg tggcccgtgt cgagaaggcg ctgcggcgcg tgcccggcgt
     1621 gcgggaggcc aacgttaacc tggccaccga gtcggccacc gtcgccgccg gggccgacac
     1681 gccgcgcgag gcgctggtca aggcggtgga ggaggcgggc tatggggtgc cgcgcgagga
     1741 ggttgcgctc ggcatcgagg ggatgacctg cgcttcctgc gtgggacgcg tggagaaggc
     1801 gctgcatgcg gtacccggtg tcctgaacgc ctcggtgaac ctcgccaccg agcgggcgca
     1861 ggtgcaaact gccggcggcg tgccggtgga cgcgctcctg acggcggtgg agaaagccgg
     1921 ttaccggggt acgcctgcga gcaccgcgcc ggccgcgagc gctgcggtcg cgaagcagcg
     1981 ttacgactgg tggcccgtcg cgctcgcggc cctgctgtcg ctgccgctca tgataccgat
     2041 gctcaccgcg cccttcggtg tgcattggat gctgcccgga tggatgcagt ggctgctcgc
     2101 gaccccggcg cagttctggc tcggcgcgcg tttctaccgc gccggctgga aggcgctgcg
     2161 cgcgggcacc gggaacatgg acctgctggt cgcgatcggc acgagcgccg gctatgcgct
     2221 gagcgtgtat cacctgctcg ccgccggcca ggagcagatg ggtcacctct acttcgaggc
     2281 ctccgcggtg gtgatcacgt tggtgctgct cggcaagtgg ctcgagacga gggccaagcg
     2341 gcagaccacc gaggcgatcc gcgcattgca ggctttgcgt ccggagcgtg cgcgtgtgcg
     2401 gcgcgacggc cgagaacgcg aggtaccggc ggaagccgtg cgcgccggcg acgaggtgat
     2461 cgtgcgcccc ggcgagcgca ttccggtgga cggcgaagta ctggagggcc gcagccatgc
     2521 cgacgagtcg ctgatcaccg gcgagagcct gccggtagcg aaggcgcccg gcgatcgcgt
     2581 gacgggcggc tcggtcaacg ccgaaggact gctggcggtg cgcaccacgg cggtcggtgc
     2641 ggagaccacg cttgcgcgca tcatccggct ggtcgaatcg gcgcaggcgc acaaggcgcc
     2701 gatccagcgg ctggtggatc gggtgagcgc ggtattcgtg ccggtggtga tcggcatcgc
     2761 cgtggcggcg ctgctcggct gggggtcggc caccggcgac tggacgcagg cgacgctcaa
     2821 cgccgtcgcc gtgctggtga tcgcctgtcc ctgtgcgttg gggctcgcca cgcccacggc
     2881 catcctcgcg ggcaccgggg tggcggcgcg cgccggtatc ctgatcaagg atgccgaggc
     2941 gctggaaatc gcgcacggcg tgcgcaccgt ggcgttcgac aagaccggca cgctgaccga
     3001 aggccggccc ggcttggtcg cgctggcgac ttcgcctgga gtcgcagagg gcgacgcgct
     3061 ggcgtgggcg gcggcgctcc agaagggcag tgagcacccg ctcgcaaggg ccgtcctggc
     3121 ggcg
//
LOCUS       DASPGE010000362         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_328671, whole genome shotgun sequence.
ACCESSION   DASPGE010000362 DASPGE010000000
VERSION     DASPGE010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328671"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VD791_11715"
     CDS             <1..429
                     /locus_tag="VD791_11715"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXV08691.1"
                     /translation="DDSTLENGCLRIVRGSHRMQTIYHHRLDERPDHALDQRIDEAEF
                     DTAQIVDVQLRAGEMSIHDVYAIHGSRPNTSPKRRVGFAIRYMPATSLYDRAIKMTGA
                     SRGLRQDMSRRPIYLVRGRDRAGNDFECGQDVPFAVGVAD"
     gene            complement(485..973)
                     /locus_tag="VD791_11720"
     CDS             complement(485..973)
                     /locus_tag="VD791_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV08692.1"
                     /translation="MAGAFRIRSATAGDVAVILDLVRGLAEYERLSHLVSATEDGVRE
                     ALFGEHPGAEAVLAFEGETAVGFAVYFHNFSTFLGRKGLWLEDLFVKPQHRRRGYGKA
                     LLLHVARIAHERGCGRFEWAALDWNAPAWEFYRALGAVPLEDWTMFRVTGETLEKLAR
                     GA"
     gene            complement(994..2790)
                     /locus_tag="VD791_11725"
     CDS             complement(994..2790)
                     /locus_tag="VD791_11725"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV08693.1"
                     /translation="MIRRLMAAVALLAPFSVHAQGGAVDDARLLGAAADRDSWITYGR
                     DYSNQRFSPLAQIRADNVQRLRPAWIFQGGANATFQATPIVVDGVMYLSLPFNHVVAL
                     DAASGKELWRYQHKRRTDKMCCGPGNRGVAVAYGKVFLGTVDARLVALDQKTGAPVWD
                     VPLVEDTAGETERTEQLAADDPLRKARVTGSTGVGASMAPLVYRGKVIIGITGVGYGL
                     HLDSDRPGAPLGAVVGIAGQSQRAGFYAAFDASTGRRLWQFDSTRASGWEGEFRTTTP
                     DGAALDRNIAQEKAAATQHRDAWKRGGGSAWTTPAVDPQLGLVFLGIGNPSPQMDDVT
                     RPGDNLYTVSLVALDVETGALRWYYQQVPHDMWGYDVASPPLLIDVQHDGAGVPAVAQ
                     AGKTGWLYVLDRRNGRLLYKSEAFVPQSNLFKRPTAEGVVIAPGGGGGANWSPLSYDA
                     RTGLVYVPALHMPMRYTVREIPATADKPALRYTALEPVDGPRWGTLSAIDTRDRGKIR
                     WQVKTPDPLLGGVLATAGDVLFTGEGSGSFSAFHARTGERLWQFNCGAGVNAPPVTYQ
                     VGGKQYVAVAAGGNAIFGFKQGEAVIAFALPD"
     gene            complement(2787..>3128)
                     /locus_tag="VD791_11730"
     CDS             complement(2787..>3128)
                     /locus_tag="VD791_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08694.1"
                     /translation="LTFNYSEQVVRAALAGHGVALGRLPLILDLLREGGLVAPFATRA
                     PIDRAYFLMQADFARGRPEVSRFVQWLIEAARRDVERPPVPEATRARAPRSATMARPG
                     RRARSTRGRKR"
BASE COUNT          472 a         1136 c         1078 g          442 t
ORIGIN      
        1 gacgactcga ccctcgagaa cggctgcctg cgcatcgtcc gcggatcgca tcgcatgcag
       61 acgatctatc accatcgtct ggacgagcgc cccgatcacg cgctggacca gcgtatcgac
      121 gaggccgaat tcgacacggc ccagatcgtg gatgtgcagc tgcgggccgg ggagatgtcg
      181 atccatgacg tttacgcgat ccacggctcg cgcccgaaca cttcgcccaa acgcagagtg
      241 gggttcgcca ttcgctacat gcccgccacg tctctctacg accgggcgat caagatgacc
      301 ggcgcatcgc gtggcttgcg ccaggacatg tcgcggcggc cgatctacct ggtgcgcggg
      361 cgggatcggg cgggcaacga cttcgagtgc gggcaggacg tgccgttcgc cgtgggagtg
      421 gccgactgac gcgaaggcaa gcgggcagcc aggcagcgcc ggatcgcggc gcgctgcatc
      481 cgggctacgc gccgcgcgcc agcttctcca gcgtctcgcc cgtcacccgg aacatggtcc
      541 agtcctccag cggcacggcg ccgagcgcgc gatagaactc ccaggccggc gcattccagt
      601 cgagcgccgc ccactcgaag cggccgcagc cgcgttcgtg cgcgatgcgg gcgacgtgca
      661 gcagcagcgc cttgccgtag ccgcggcgcc ggtgttgcgg cttgacgaac aggtcctcca
      721 gccacaggcc cttgcgcccc aggaacgtcg agaagttgtg gaagtacacg gcgaacccga
      781 ccgccgtctc cccctcgaag gcgagcaccg cctccgcgcc cgggtgctct ccgaacaggg
      841 cctcgcgcac gccgtcctcg gtggcgctca ccaggtggct caggcgctcg tattcggcca
      901 gcccgcgcac caggtcgagg atgacggcga cgtcgccggc cgtcgcggac cggatgcgaa
      961 atgcgcctgc catgacgatc tgatccgggg ccgtcaatcc ggcaacgcga aggcgatcac
     1021 cgcctcgccc tgcttgaagc cgaagatggc gttgccgccg gcggcgaccg cgacgtactg
     1081 cttgccgccg acctggtagg tgaccggcgg cgcgttgaca cccgctccgc aattgaactg
     1141 ccacaggcgc tcgccggtgc gcgcgtggaa tgcgctgaag ctgccgctgc cctcgccggt
     1201 aaacagcacg tcgccggcgg tggcgagcac cccgcccagc aacggatccg gggtcttcac
     1261 ctgccagcgg atcttgccgc gatcgcgcgt gtcgatcgcg ctcagcgtgc cccagcgcgg
     1321 cccgtccacc ggctccagcg ccgtatagcg cagcgccggc ttgtcggcgg tggcgggaat
     1381 ctcgcgcacg gtgtagcgca tcggcatgtg cagcgccggc acgtacacca ggccggtgcg
     1441 cgcatcgtag gacagcggcg accagttggc gcccccgccg ccgcccggcg cgatcaccac
     1501 cccctcggcg gtgggccgct tgaacagatt cgattgcggc acgaacgcct cggacttgta
     1561 cagcagccgg ccgttgcgcc gatccagcac gtacagccag ccggtcttgc cggcctgcgc
     1621 caccgccggc acgccggcgc catcgtgctg cacgtcgatc agcagcggcg ggctggcgac
     1681 gtcgtagccc cacatgtcgt gcggcacttg ctggtaatac cagcgcagcg cgccggtctc
     1741 cacgtccagc gccaccagcg acacggtgta gaggttgtcg cccgggcggg tgacgtcgtc
     1801 catctgcggc gagggattgc cgatgccgag gaacaccagg ccgagctgcg gatcgaccgc
     1861 cggggtggtc caggccgagc cgccgccgcg cttccaggca tcgcggtgct gcgtagccgc
     1921 ggccttctcc tgcgcgatgt tgcggtcgag cgcagcaccg tccggcgtgg tcgtgcggaa
     1981 ctcgccttcc catccgctcg cgcgcgtcga atcgaactgc cacagccggc ggccggtgct
     2041 ggcgtcgaac gcggcgtaga agccggcacg ctggctctgg ccggcgatgc cgaccaccgc
     2101 gccgagcggg gcgccggggc gatccgagtc gagatgcagg ccgtaaccga cgccggtgat
     2161 gccgatgatc accttgccgc ggtagaccag gggcgccatg ctggcgccga ccccggtgga
     2221 gccggtcact ctcgccttgc gcaggggatc gtcggcggcg agctgctcgg tgcgctcggt
     2281 ctcgcccgcc gtgtcctcga ccagcggcac gtcccatacc ggcgcgccgg tcttctggtc
     2341 gagcgcgacg aggcgtgcgt ccaccgtgcc gaggaacacc ttgccgtagg ccaccgccac
     2401 gccgcggttg cccggtccgc agcacatctt gtcggtgcgg cgcttgtgct ggtagcgcca
     2461 cagctccttg ccgctggccg cgtccagcgc gaccacgtga ttgaacggca gcgacaggta
     2521 catcaccccg tcgaccacga tcggcgtggc ctggaaggtg gcgttcgcgc caccctggaa
     2581 gatccacgcc ggccgcaggc gctggacgtt gtccgcacgg atctgggcga gcggcgagaa
     2641 gcgctggttg ctgtagtcgc gcccgtaggt gatccagctg tcgcgatccg cggcggcgcc
     2701 gagcaggcgc gcatcgtcca ccgcaccgcc ctgcgcatgg accgaaaacg gcgccagcaa
     2761 cgcgacggcc gccatcaggc gtcgaatcat ctcttcctcc ccctggttga acgggcgcgg
     2821 cgcccgggac gcgccattgt cgcagaccgc ggcgcacgcg cgcgggtggc ctccggcacc
     2881 ggcggtcgct cgacatctct gcgcgccgcc tcgatcagcc attgcacgaa gcgcgagacc
     2941 tcgggacggc cgcgcgcgaa gtcggcctgc atcaagaaat aggcgcgatc gatcggcgcg
     3001 cgcgtggcga acggcgcgac cagcccgccc tcgcgcagca gatcgaggat cagcggcaac
     3061 ctgcccagcg ccaccccgtg cccggccagg gcggcgcgaa cgacctgctc ggagtagttg
     3121 aaggtgag
//
LOCUS       DASPGE010000363         4006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_913056, whole genome shotgun sequence.
ACCESSION   DASPGE010000363 DASPGE010000000
VERSION     DASPGE010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913056"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VD791_11735"
     CDS             complement(<1..656)
                     /locus_tag="VD791_11735"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08695.1"
                     /translation="MTDFQRPDPRLLEQVRAVAKCYSASCVFADVQQRGGVMHSALKP
                     IFNGKAVGPALTVKLSPGDLQDPLLALHVAQPGDVVVVDAGGETETSVFGGLMGSLFK
                     NRGVQGAVIDGACRDTDELRDIGFLVFSRAVTARGTHTMFSGRKTDVQVSVPVACGGI
                     VVRPGDVIVGDEIGVTVVPAADLEKVLAAAREQAEREEATRQRIRQGKTFEELLAEFG
                     R"
     gene            complement(708..1271)
                     /locus_tag="VD791_11740"
     CDS             complement(708..1271)
                     /locus_tag="VD791_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV08696.1"
                     /translation="MLAASAAHAQGDYEAGKQKAQACAACHGADGNAPKGVAPEGQAY
                     PDLAGQTARYTYLQLKDFKEGRRKNPLMSPMAANLSRQDMFDLAEYFSKQKHLPSDFK
                     TDPAKVAEGKRVADAALCTMCHLGGFSGQNEIPRAAGQEYDYIVKQLQDFRARNRTND
                     AGNMTSVSRGLTDEQIDALGHYITGLR"
     gene            complement(1424..3145)
                     /locus_tag="VD791_11745"
     CDS             complement(1424..3145)
                     /locus_tag="VD791_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV08697.1"
                     /translation="MKSTNLKLLALALTASGVVGGAIAQEVSGGATTTFAQMAADVRV
                     SQEMLAASEGDKSNWLQTNGGWSNARYAAGDQIHVKNVAKLRPVFIVQTEIKESMETA
                     PVVVNGVMYITTSYNHIYAVDAATGKQFWHYKHKMGPVTTYCCGPNNRGVAIYRDMVY
                     MGTLDAKLIGLDAKTGKLLWEKQIADPELGYSETMAPVAADGKILIGTNGGEYGVRGF
                     VKAYDARNGELLWTFYTIPEKGHEGVWAVNDATGRNMLRDIAKEKETLAEKGGDFYQT
                     LGGGVWMTPAVDMRTKTVYFVAGNPSPDLYGDIRPGDNLYTNSIVAVDLNTGAYKAHF
                     QYIAHDVWDLDAVSPPILTQAAGKDGKTMMDVVIHGGKTGHIYVHDRKDLSLIRFSDA
                     MIPQENVWVLPTPEGVRMLPGANGGVEWNPMAVNPKLRLAYAANLHQPMTYHVEPAEY
                     PGGKLWLGGAFKVIPGERQWGRLAAVNIDTGKVVWNADTEQPLMGGVLATAGGLVFTG
                     EGNGQFNAYDARNGKKLWTFQCGAGVNAPPVSYMVGGKQYIAVAAGGNTQLDFKRGNN
                     VIVFALP"
     gene            3275..3937
                     /locus_tag="VD791_11750"
     CDS             3275..3937
                     /locus_tag="VD791_11750"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXV08698.1"
                     /translation="MRSSIVGTALALALAGLLPPAQAQTPTPEKSLNAQLLDFAKRGE
                     EQRARSVLDAGAAPNSRNRRGETALYMFARDGRLAAVIMLLEHGANPNLAALDRTTPL
                     MAAAANGHTEAVRALIGAGADVHALDQIAKTALLYAAAGGHVQAMDLLLDRGIDVNAR
                     YKHRLTALMWAAGSGATASVKRLLERGADPALEDDRGKTAADIARESRHAEVAELLAK
                     AM"
BASE COUNT          592 a         1374 c         1351 g          689 t
ORIGIN      
        1 cggccgaact cggccagcag ctcctcgaac gtcttgccct ggcggatgcg ctggcgtgtg
       61 gcctcctcgc gctcggcctg ctcgcgcgcg gcggcgagga ctttctcgag gtcggcggcg
      121 gggaccacgg tgacgccgat ctcgtcaccg acgatcacgt ctcccgggcg caccacgatg
      181 ccgccgcagg cgaccggtac gctcacctgc acgtcggtct tgcgcccgga gaacatggtg
      241 tgggtgccgc gcgcggtgac ggcgcgcgag aacacgagga agccgatgtc gcgcagctcg
      301 tcggtgtcgc ggcaggcgcc gtcgatcacc gcgccctgca cgccgcggtt cttgaacagg
      361 ctgcccatca ggccgccgaa caccgatgtc tcggtctcgc cgccggcgtc gaccacgacc
      421 acgtcgcccg gttgcgcaac gtgcagggcg agcaacgggt cctgcaggtc gccgggcgag
      481 agcttcacgg tgagggccgg ccccaccgcc ttgccgttga agatcggttt gagcgcgctg
      541 tgcatcacgc cgccgcgctg ctgcacgtcg gcgaaaacgc aggaggcgct gtagcacttc
      601 gccactgcgc gcacctgctc cagcaatctc gggtcggggc gctggaagtc ggtcatcgga
      661 cggtcctgtg aaggcaaagg ctgcgcccct gggcgcgcgg cgccggctta ccggagaccg
      721 gtgatgtaat gccccagcgc gtcgatctgc tcgtcggtga gcccgcgcga cacgctggtc
      781 atgttgccgg cgtcgttggt gcggttgcgg gcgcggaaat cctgcagctg tttgacgatg
      841 taatcgtact cctggccggc ggcgcgcgga atctcgttct gcccggagaa gccgcccaga
      901 tggcacatcg tgcacagcgc ggcgtcggcc acgcgcttgc cctcggcgac cttggccggg
      961 tcggtcttga agtcgctcgg caggtgcttc tgcttggaga agtactcggc cagatcgaac
     1021 atgtcctgcc tggatagatt cgccgccatc gggctcatca gcgggttctt gcggcggccc
     1081 tccttgaaat ccttgagctg caggtaggtg tagcgggcgg tctggcccgc cagatccgga
     1141 taggcctgac cttccggcgc cacccccttg ggggcgttgc cgtcggcccc gtgacacgcg
     1201 gcgcacgcct gcgccttctg cttacccgcc tcgtagtcgc cctgcgcgtg cgcagcgctc
     1261 gcggccagca tgggggccgc ggcgacggcg aaggcgagaa tccagaaacg cttcatcgta
     1321 agctcttccc gaggagaccg gaacgtccca aacgcgttgg gcaagaaaaa ggccgcgccc
     1381 ggatcaacgc acgggcacgg ccccggatga cgcgcggcgc cgatcagggc agcgcgaaca
     1441 cgatgacgtt gttgccgcgc ttgaagtcga gttgcgtgtt gccgcccgcg gccacggcga
     1501 tgtattgctt gccgccgacc atgtaggaca ccggaggcgc gttcacgccg gcgccgcact
     1561 ggaaggtcca cagcttcttg ccgttcctgg cgtcataggc gttgaactgc ccgttgccct
     1621 cgcccgtgaa caccagcccg ccggcggtgg ccagcacgcc gcccatcagg ggctgctcgg
     1681 tgtcggcgtt ccacaccacc ttgccggtgt cgatgttcac cgccgccagc cgaccccact
     1741 gcctctcgcc cgggatcacc ttgaaggcgc cgcccagcca cagcttgccg ccggggtact
     1801 cggcaggctc cacgtggtag gtcatcggct ggtgcaggtt ggccgcgtag gccaggcgca
     1861 gtttcgggtt gaccgccatc gggttccatt ccactccgcc gttggcgccc ggcagcatgc
     1921 gcacgccctc gggggtgggc agcacccaca cgttctcttg cgggatcatg gcgtcggaga
     1981 agcggatcag ggacaggtcc ttgcggtcgt gcacgtagat gtgaccggtc ttgccgccgt
     2041 ggatcaccac atccatcatc gtcttgccgt ctttgccggc ggcctgggtc aggatcggcg
     2101 ggctcaccgc gtccaggtcc cacacgtcgt gcgcgatgta ctggaagtgg gccttgtagg
     2161 cgccggtgtt caggtccacc gcgacgatcg agttggtgta caggttgtcg ccgggacgga
     2221 tgtcgccgta caggtcgggc gaggggttgc ccgccacgaa gtacacggtc ttggtgcgca
     2281 tgtccaccgc cggcgtcatc cacacgccac cgcccagcgt ctggtagaag tcgccgcctt
     2341 tctccgcgag ggtttccttc tccttggcga tgtcgcgcag catgttgcgg ccggtggcgt
     2401 cgttcaccgc ccagacgccc tcgtgcccct tctcggggat ggtgtagaag gtccacagca
     2461 gctcgccgtt gcgggcgtca taggccttga cgaagccgcg cacgccgtac tcgcccccgt
     2521 tggtcccgat caggatcttg ccgtctgccg ccaccggggc catggtctcg gagtagccca
     2581 actcgggatc ggcgatctgc ttctcccaca gcagcttgcc ggtcttggcg tcgagcccga
     2641 tcagcttggc gtccagcgtg cccatgtaca ccatgtcgcg gtagatcgcg acgccgcggt
     2701 tgttcggtcc gcagcagtac gtggtgaccg gccccatctt gtgcttgtag tgccagaact
     2761 gcttgccggt cgccgcgtcg accgcgtaga tgtggttgta cgacgtggtg atgtacatca
     2821 cgccgttgac cacgaccggc gcggtctcca tcgactcttt gatttccgtc tggacgatga
     2881 agaccgggcg caacttggcg acgttcttga cgtggatctg gtcgcccgcg gcatagcgcg
     2941 cgttgctcca gccgccgttg gtctgcagcc agttgctctt gtcgccctcg ctggcggcca
     3001 gcatctcctg gctgacgcgg acgtcggccg ccatctgagc gaacgtcgtc gtggccccac
     3061 cgctgacctc ctgagcgatg gcgcccccca cgacgccgga cgcggtcagc gccagggcga
     3121 gcagcttcag attggttgac ttcatgactc ctcccgggaa atggttcgta acttcaggcg
     3181 ccttgcaccg catcatggcc ggcaaggccg ggccggcgcg gcagcgagac tataatgaac
     3241 ctttcacatt tgctcaagca caccctccga accgatgcgg tcatcgatcg tgggcaccgc
     3301 cctggcgctg gcgctcgccg gcctgttgcc gccggcgcaa gcgcagactc ccacccccga
     3361 gaagagcctc aacgcgcagc tgctggattt cgccaagcgc ggcgaggagc agcgcgcacg
     3421 cagcgtcctg gacgccggcg ccgcgcccaa ctcgcgcaac cggcgcggtg aaaccgcgct
     3481 gtacatgttc gcccgcgacg gccggctggc agccgtgatc atgctgctcg agcacggcgc
     3541 gaacccgaac ctcgccgcac tggacaggac cacgccgttg atggcggcgg ccgccaatgg
     3601 ccacacggag gcggtgcgtg cgttgatcgg cgcaggcgcc gacgtgcacg cgctcgacca
     3661 gatcgccaag acggcgctgc tgtacgcggc ggcgggcggc cacgtccagg ccatggacct
     3721 gctgctcgac cgcggcatcg acgtcaatgc acgctacaag caccggctca ccgcgctgat
     3781 gtgggcggcg ggctcggggg cgaccgccag cgtgaagcgc ttgctcgagc gcggcgccga
     3841 ccccgcgctc gaggacgacc gcggcaagac cgccgcggac atcgcgcgtg agagccgcca
     3901 cgcggaggtg gcggagctgt tggcgaaagc gatgtaagtg cgcttgaatc gtagcccgga
     3961 tgcagcgcag cggaatccgg gagaagagag gccttgttct tgcccc
//
LOCUS       DASPGE010000364        13726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_679381, whole genome shotgun sequence.
ACCESSION   DASPGE010000364 DASPGE010000000
VERSION     DASPGE010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13726
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679381"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VD791_11755"
     CDS             complement(<1..227)
                     /locus_tag="VD791_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08699.1"
                     /translation="MNRVARRPLLPMLPRCLLVLGALAALSLPARAGDVSAQAEAVWR
                     KSFSTASPDEWAARLAQDEVNALCSKHRNNPP"
     gene            complement(239..1045)
                     /gene="soxA"
                     /locus_tag="VD791_11760"
     CDS             complement(239..1045)
                     /gene="soxA"
                     /locus_tag="VD791_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HXV08700.1"
                     /translation="MPTIHRRLAAVAAGACLTLAVSAAGQDKAMEEIQRYRQMLQEGN
                     PAELMEMNGEALWTTPRGPRGATLERCDLGLGPGKLEGTYAQLPRFFADTGKVQDAES
                     RLVTCMTTLQGLSIEEATKNWHRPGSDIEALVTFVATKSKGAKIDVPARHPREAETFA
                     VGEELFFRRSGPLDFSCATCHSQDDKRIRLQELPNLTTAQGAQASMAQWPAYRVSQSA
                     VWTMERRLIDCMRQMRFPDADYLSDAVVALQVYMQKKASGATLEAPGIKR"
     gene            complement(1172..1483)
                     /gene="soxZ"
                     /locus_tag="VD791_11765"
     CDS             complement(1172..1483)
                     /gene="soxZ"
                     /locus_tag="VD791_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HXV08701.1"
                     /translation="MGNPMKIRAKAEGDVVEVKVLMSHIMETGQRKNQAGEPIPAHFI
                     QTVNATCNGRSVLSAQWGPAVSANPFLSFKFKGGQKGEKVTVTWVDSKGDSRSDETVI
                     N"
     gene            complement(1524..1988)
                     /gene="soxY"
                     /locus_tag="VD791_11770"
     CDS             complement(1524..1988)
                     /gene="soxY"
                     /locus_tag="VD791_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HXV08702.1"
                     /translation="MEKSRREFLRSSGGLLGLLLAAGFLRPEEVAAEEWNKAAFETKK
                     LEDLVKVLGGNTAQESAEVAIVAPDIAENGAVVPVGVVSKLDKTSSIAVLVEKNPNVL
                     AATFVIPQGTLPELQTRVKMAQTSNVFALVKADGKFYYAAKEIKITLGGCGG"
     gene            complement(2014..3018)
                     /locus_tag="VD791_11775"
     CDS             complement(2014..3018)
                     /locus_tag="VD791_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV08703.1"
                     /translation="MFSVAHAFPCIALGAGDKPWSGLGRAATPREIAAWDIDVKPDGS
                     GLPKGRGGVAQGQEIYDAKCASCHGTFGESNQYMQLAGGVGSLRSDNPVRTTGSKLNY
                     AATLWDYINRAMPFQAPKSLTADEVYALTAYLLYLNEIVPEDATLDQTSILQVRMPNR
                     DGFTTQHGFMRRDGKPDVQAGACMRDCSVEGKVSSRIPDYARNAQGNLATQFRVVGPY
                     RGADTSKPEPMGPLAAARQALAEPAVAASAPKQSAADLAKHYACIACHGLEQKIVGPG
                     FKEVAQKYAGDATARQALMRKVRDGGAGVWGAIPMPGQPQVAEADLVAIIDWVLAGAK
                     "
     gene            complement(3113..4420)
                     /gene="soxC"
                     /locus_tag="VD791_11780"
     CDS             complement(3113..4420)
                     /gene="soxC"
                     /locus_tag="VD791_11780"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HXV08704.1"
                     /translation="MSGRRRIRPAPENFLTPEDIARRRFLRRSLALGGALTAAGAAGV
                     AAAQAPDPILQLPEHSKTLGQPVAARGYGMPSKHESNVQRRESPGLTRTSISSIAFAP
                     LQSLFGIITPSGVHFERHHAGWHDIDPAQHRLMIHGLVRQAKVYTMDDLMRLPSVSRI
                     HFIECGANTGMEWGNVAVPTVQYTHGMLSCSEFTGVLLSTLLDDVGFDAAKARFVLAE
                     GADGASLTRSVPIDMALDDVMVVYGQNGEMLRPENGYPLRLLVPGVQGVSSVKWLRRL
                     EVGHEPWNTREEALHYIDLMPDGIHRQYTWINECKSVITAPSGGQVLLDRGYYEITGL
                     AWSGRGRVRRVDVSVDGGRNWREARLQEPVLTKCLTRFRCDWVWDGSPAIVQSRAIDE
                     TGYVQPQIRQLRAVRGTRAIYHNNAIQSWQIAENGEVSNVQVY"
     gene            complement(4473..4832)
                     /locus_tag="VD791_11785"
     CDS             complement(4473..4832)
                     /locus_tag="VD791_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXV08705.1"
                     /translation="MHEVDEVLESVASYFSLLSAPTRLKVLHAICERELSVGEIVKET
                     GFTQTNVSRQLSALYARGVLARRKEGSMVFYRVTDTTLVELCRAACVRIASRIEERRP
                     LKRALLKFMPGNAQRQR"
     gene            complement(5036..6325)
                     /locus_tag="VD791_11790"
     CDS             complement(5036..6325)
                     /locus_tag="VD791_11790"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HXV08706.1"
                     /translation="MRRIRSSLAAVLVCSACGALHAQELDSPLVAASLLEALRDGELL
                     LELRPRYTWVDQDGRPEEARWGSLRTRLGWKTLEYRGFSAVIEGISIARFAESGAIDY
                     DDTPGVGPGSVVALYGAGYYPLVADEPTTDFNRLYLDYVGVPHTLIRAGRQLVRIDNQ
                     RFVGDYDFAQLPQAFDGLLVENASLSQVRLTAGYFAQVRNTFAERTDTDTVVLNARYE
                     PWPLIKVAGYAYLQDQPYTGSATGFADSSNQILGGRLWGGWKATGALELLYSAEFAEQ
                     RDYADGDPRIDAPYRRVGAGVIAGVAAKRGYLKIERELLGSNEGLYGFQTPLGSTQLF
                     TGRADIFATTPARGLEDRRAQIGVQVSKATLHLEYHRFRADFREWDLGTEWDFGFAWA
                     FTPRLSAGVEYADYHAGDPRAALADTRKVWLTIGYRH"
     gene            complement(6410..7432)
                     /gene="ybiB"
                     /locus_tag="VD791_11795"
     CDS             complement(6410..7432)
                     /gene="ybiB"
                     /locus_tag="VD791_11795"
                     /inference="COORDINATES: protein motif:HMM:NF006005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HXV08707.1"
                     /translation="MRTMQVIKQIAQPGGSVRDLAEGEAHELFGGMLDGGLPDLELGA
                     VLIALHLKSESGGELLGFQRALRERLYPLRAPTARARPLLLPAYGGARTEHNLLPLLG
                     LLLRRLGVPVLFHGTLEGSGRLASVYILREFGVLPSASLLQAQARLDAEHITFVPTAV
                     LCPGLAALLALRNRLGVRNSAHLVAKLLDPFGGHSVLVIGASRPQTLEQLAGVLLELG
                     ACALLLASTEGEPFASPRRRPRIEHFRRGVRQTLFEEEAGPVRPVAGLPAGIDAHATA
                     QWMRLALAGEAPIPYPLVNQLACCLYACGYTDDMNQAKAIAAVEAGSLAPGAQGGHRR
                     GVQHSA"
     gene            7716..8513
                     /locus_tag="VD791_11800"
     CDS             7716..8513
                     /locus_tag="VD791_11800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HXV08708.1"
                     /translation="MNSWTCLLAAAAAFLPLSAAHAKGIRLPASADRQFETLRLDSAL
                     SDLRAPATAAAQGETDAVFAAGHAHPLDVQTQEVASGMYRPLAENLSTLLETSYARGS
                     GLTAEWSVLGQVGARFGDGWGVRAGVRHSEQELATILLPPTLAPATADLGMLTFERQW
                     NRYRGEYTYYAGRSDAGASASGHRIQLHYYYSPRSSVGLAYHTSRQLDAGGELGTFNP
                     ASEVANVGVVGEHWFSPTWALNYNALVEDGGNQGLNPELRLGLRVRF"
     gene            8770..9855
                     /locus_tag="VD791_11805"
     CDS             8770..9855
                     /locus_tag="VD791_11805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV08709.1"
                     /translation="MPPPEVSAVTVAPRDIAITLEYVGRTEGVREVEVRPRVSGILQR
                     WNYTEGSHVKAGQSLFTIDPAPFQAVVARAEAELASARARVAQAQRDLERLKPLLDQG
                     MVSRKAYDDAIAADEVAVAQLQGAEAALSEAKLDLKYTRVEAPISGVTSRALKSEGSL
                     VEAQNTLLTSISQIDPIRVIFSMSEAERLRFNEQAAAGKLRLPRDNRFAVTVVLPDGS
                     TSERSGVVEFSDIRVDPATGTTEFRAVIPNPDHRVRPGQFVRVTLRGAQHVQALAVPQ
                     RAVLEGPQGKIVLVVDDKGMAQPRPVQVGEWAGEDWVISDGLKPGERVIVDGVVKVRP
                     GMPVKIAETPPAPQAAAPEQQQPTAKP"
     gene            9917..13159
                     /locus_tag="VD791_11810"
     CDS             9917..13159
                     /locus_tag="VD791_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HXV08710.1"
                     /translation="MNVSRFFIDRPIFAAVLSAFLVLAGLAAIRVLPIAQYPEIAPPV
                     VTVRAIYPGASAQVLEQTVAAPLENQINGVENMIFMSSNSGSNGLLEILVTFDIGSDA
                     DQAALDVNNRVKQAEARLPEEVRRQGVTVEKGSSAFLIVYGFYSPDGRFDDLYTSNYV
                     TLNVLDAIKRVPGTTNVQIFGAKDYAMRIWVLPERLTQLQLTTDDLVRAITEQNAQFA
                     AGKIGQTPSGGPQELVYTITTRGRMSEVSEFENIIVRALPGGAKVRLKDVARVELGSK
                     DYEFIGRVNGKQATLMGIFLQPGANALDVAEECDAVMAQLAKRFPEGLTYANVYDTTR
                     FVKVSIREVVKTLAEAMALVFLVVFVFLQNWRATLIPFAAVPVSLIGTFAGLQLMGYS
                     INTLTLFGMVLSIGIVVDDAIVVLENVERIMHEQHRDAREAAVQAMHEVTGPVVAIVL
                     TLCAVFVPIAFLGGLTGELYRQFAVTISIAVVISGFVALTLTPSLCVLILKRRHRPPG
                     PFFRWFNEAFRRVTARYSDGVGWMLRRGAIGMALFAGMVAITAWLWRSTPTSLVPDED
                     TGYYIGAVFLPDGATLQRTDAVVGKVTEAMLSNPANQYAVAFTGLDFLGGGFRNSAAT
                     IFASHRHWDDRPGVYTPQLVGEFFAKTAHIKEGLVLAFAPPPIFGLGNAGGFEFYIQN
                     RGEGGPQRLAEVMGQFLARANADPMLGAGGGWVQTLWRPNVPQLFVDLDREKAKALGV
                     PINALYSSIAATLGTYYVNDFNKFGRTWQVLMSADPVYRKRPDSIGEIYVRSEHGEMI
                     PLKALAEVRYSSGPDSLDRFNNLPAVKLLGQGAPGVSSGQAIQAVERIAEEVLPAEFS
                     YDWGGASFQEKRSAGTSAIALGLAVIMVFLILAAQYERWSLPFSVLMALPFGTFGALA
                     AIWINNLTLPFTGRTPLANDVYFQIGLVTLLGLAAKNAILIVEYAVLKKQEGLSTCGS
                     ALEAARLRFRPILMTSLAFILGVAPLAFSTGAGAGARLAVGTGVMGGMLAATFLAIFF
                     IPLFFKLMTDGHLRERRSTGEIRTEIEHHRALGEHPIDRPGHHPPALGTA"
BASE COUNT         2080 a         4803 c         4619 g         2224 t
ORIGIN      
        1 ggcggattgt tgcggtgctt gctgcacagc gcgttgactt cgtcctgggc gaggcgcgcg
       61 gcccactcgt ccgggctggc ggtggagaac gatttgcgcc agacggcttc ggcctgtgcg
      121 gaaacgtcgc cggcgcgcgc gggcagcgac agcgccgcaa gcgcccccag cacgagaagg
      181 cagcgcggaa gcatcggcag cagcgggcgg cgggcgactc gattcatgtc ccgtctcctc
      241 agcgcttgat gcccggcgct tccagggtcg cgccgctcgc cttcttctgc atgtacacct
      301 gcagcgcgac caccgcgtcg gacaggtagt ccgcgtcggg aaagcgcatc tgccgcatgc
      361 agtcgatcag gcggcgctcc atggtccaca ccgcgctctg cgacacgcgg taggccggcc
      421 actgcgccat cgaggcttgc gcgccctgcg ccgtggtgag attgggcagc tcctgcagcc
      481 ggatgcgctt gtcgtcctgg ctgtggcagg tggcgcagga gaagtcgagc ggccccgagc
      541 ggcggaagaa caactcctcg cccacggcga aggtttccgc ctcgcgcgga tggcgggcgg
      601 gcacgtcgat cttcgcgccc ttcgacttgg tggccacgaa cgtcaccagc gcctcgatgt
      661 cggagcccgg ccggtgccag ttcttggtgg cttcctcgat cgacaggccc tgcagcgtgg
      721 tcatgcaggt caccaggcgc gactccgcgt cctgcacctt gccggtgtcg gcgaagaagc
      781 gcggtagctg cgcatacgtg ccctcgagct tgcccggccc caggccgagg tcgcagcgct
      841 cgagcgtcgc gccacgcggg ccgcgcggcg tcgtccacag cgcttcgccg ttcatttcca
      901 tcagctccgc cgggttgccc tcctgcagca tctggcggta gcgctgaatc tcttccatgg
      961 ccttgtcctg gccggcggcg gacacggcca gcgtgaggca cgcgcccgcc gcaacggcag
     1021 cgagccggcg atgaatcgtg ggcatgcggt tcctcgtcac cttcgtaatg cggatccggc
     1081 gtggtgcaaa tgcggtgcgg tgcgatcgac gagcgcccgg attccgcttc gctgcatcca
     1141 ggctacgcga cttcgtcgcg cctggccacc ctcaattgat caccgtctcg tccgagcgcg
     1201 agtcgccctt gctatccacc caggtcaccg tgaccttctc gcccttctgt ccgcccttga
     1261 acttgaagga caggaagggg ttggcggaca ccgccggtcc ccactgcgcg gacagcacgg
     1321 agcggccgtt gcaggtcgcg ttcaccgtct ggatgaaatg cgcgggaatc ggctcgcccg
     1381 cctggttctt gcgctggccc gtctccatga tgtggctcat cagcaccttg acctccacca
     1441 cgtcgccctc ggccttggcg cgaattttca tcgggtttcc catttcgtta cctcttaagc
     1501 ctctagtggc tcgccggcgc cggtcaaccg ccgcagccgc ccagcgtgat cttgatctcc
     1561 ttggcggcgt agtagaactt gccgtccgcc ttgaccagcg caaacacgtt cgacgtctgc
     1621 gccatcttga cgcgggtctg cagctcgggc agcgtgccct gcggaatgac gaaggtcgcc
     1681 gcgagcacgt tgggattctt ctcgaccagg acggcgatgg acgaggtctt gtccagcttg
     1741 ctgacgacgc cgacgggaac caccgcaccg ttctcggcga tgtccggtgc gacgatcgcg
     1801 acctcggcgc tctcctgcgc ggtgttgccg ccaagcacct tgaccaggtc ctccagcttc
     1861 ttggtctcga acgccgcctt gttccactcc tcggcggcca cctcttcggg ccgcaggaac
     1921 ccggcggcca acagcagtcc gagcaggccg ccgctggatc tcagaaactc ccttctcgac
     1981 ttctccatgt cgcgctcctc acgaacaacg ttctcatttc gcccctgcca gcacccagtc
     2041 gatgatcgcg accaggtcgg cttcggccac ttgcggctga ccgggcatcg ggatcgcacc
     2101 ccacacgccc gcgccgccgt cgcgtacctt tcgcatcaac gcctgccttg cggttgcgtc
     2161 gccggcgtac ttctgcgcca cctccttgaa gcccggaccc acgatcttct gctccaggcc
     2221 atgacaggcg atgcaggcgt agtgcttggc gaggtcggca gccgactgtt tcggtgcaga
     2281 cgcggcgacc gccggctccg ccaacgcctg acgggcggcc gcgagcggcc ccatcggctc
     2341 cggcttgctg gtgtcggcgc cgcggtaggg tccgaccaca cggaactgcg tcgccaggtt
     2401 gccctgcgcg ttgcgcgcgt agtcggggat gcgggaactg accttgccct cgaccgagca
     2461 gtcgcgcatg caggcgccgg cctgcacgtc cggctttccg tcgcggcgca tgaagccgtg
     2521 ctgcgtggtg aagccatccc ggttgggcat ccgcacctgg aggatcgatg tctgatccag
     2581 cgtcgcgtcc tcgggcacga tctcgttcag atacaacagg tatgccgtca gcgcgtagac
     2641 ctcatcggcg gtcagggact tgggcgcctg gaacggcatg gcgcggttga tgtagtccca
     2701 cagtgtggcg gcgtagttga gcttgctgcc ggtggtgcgc accgggttgt cgctgcgcag
     2761 gctgcccacg ccgcccgcga gctgcatgta ctggttgctc tcgccgaagg tgccgtggca
     2821 ggacgcgcac ttggcgtcgt agatttcctg cccctgcgcc acgccgccgc ggcctttggg
     2881 cagcccgctg ccgtcgggct tgacgtcgat gtcccaggcg gcgatctccc ggggcgtggc
     2941 cgcacgtccc agtcccgacc agggcttgtc ccccgcgccg agtgcgatgc acggaaaggc
     3001 gtgagccaca gagaacacag agatcaccga gaggacctgg agcagggaac tgcgcttccc
     3061 gcgcccgcgg atcgcgtctg ccgcgatatc tccgtgtacg ctccggcgag ggtcaataca
     3121 cctggacatt gctcacctcg ccgttctcgg cgatctgcca cgactggatc gcattgttgt
     3181 ggtagatcgc ccgcgtgccg cgcaccgcgc gcagctgccg gatctgcggc tggacgtagc
     3241 cggtttcgtc gatcgcccgg ctctggacga ttgcggggct gccgtcccac acccagtcgc
     3301 aacggaagcg cgtcaggcat ttcgtcagga ccggctcctg cagcctggcc tcccgccagt
     3361 tgcggccgcc gtccaccgac acgtccacgc gccgcacgcg cccgcgcccg gaccatgcca
     3421 ggccggtgat ctcgtaatag ccgcggtcga gcagcacctg tccgcccgac ggcgcggtga
     3481 tcaccgactt gcactcgttg atccaggtgt actggcgatg gatgccgtcg ggcatgaggt
     3541 cgatgtagtg cagcgcttcc tcgcgcgtgt tccacggctc gtggccgacc tcgagccggc
     3601 gcagccactt gacgctggac acgccctgca cgcccggcac cagcaggcgc agggggtagc
     3661 cgttctcggg ccgcagcatc tcgccgttct gcccgtacac caccatcacg tcgtcgagcg
     3721 ccatgtcgat gggcacgctg cgggtgagcg aggcgccgtc ggcaccctcg gccagcacga
     3781 acctggcctt ggccgcgtcg aagcccacgt cgtcgagcaa cgtggacagc agcacgccgg
     3841 tgaactcgct gcaggagagc atgccatggg tgtattgcac ggtgggcacc gcgacgttgc
     3901 cccactccat gccggtgttc gccccgcact cgatgaagtg gatgcgcgac accgacggca
     3961 gccgcatcag gtcgtccatg gtgtaaacct tggcctgccg caccaggccg tggatcatca
     4021 ggcggtgctg cgccgggtcg atgtcgtgcc agccggcgtg gtgccgctcg aaatgcacac
     4081 cgctcggcgt gatgatgccg aacaggctct gcagcggcgc gaacgcgatg gacgagatgg
     4141 aggtgcgggt gaggcccggg ctctcgcgcc gctgcacgtt gctctcgtgc ttcgagggca
     4201 tgccgtagcc gcgcgcggcg accggttgac ccagcgtttt gctgtgctcg ggaagctgca
     4261 ggatcggatc gggcgcttgc gccgctgcca cgccggcggc gcctgccgcc gtcagcgcgc
     4321 cgccgagcgc aagcgaccgg cgcaggaagc gccggcgcgc gatgtcctcg ggggtgagaa
     4381 agttctcggg cgccggccgg atgcggcggc gccccgacat cgcctggggc gtgatttcgg
     4441 gcgtgctggg gtccatgctg cgcccgcgcg acctatctct gccgttgcgc gttgccgggc
     4501 atgaacttga gcagcgcgcg cttcagcggc cgccgctcct cgatccggct ggcgatgcgc
     4561 acacacgcgg cgcggcacag ctccaccagg gtggtgtcgg tgacccggta gaacaccatg
     4621 ctgccctctt tgcgccgggc gagcacgccg cgcgcataca gggcggagag ctggcgcgac
     4681 acgttggtct gggtgaaccc ggtctccttg acgatctcgc ccaccgacag ctcgcgctcg
     4741 cagatcgcgt gcagcacctt gagccgggtg ggcgccgaca gcaggctgaa gtaggacgcg
     4801 accgattcca acacctcgtc gacctcgtgc atggggagtg cgctccgatg gaatgcgcgc
     4861 aagcatatgc gcatatacgc atatagtcaa gactgtcggt attgcgccgc agcacgctcg
     4921 ggcactgctc cccgcctctc acgaggcgga gtgcccgcga tggtgtacgg ctgcctgaac
     4981 caccccgtca cgcttcgcgt gccacccctc ctcgaagagg aggggaacct gcagatcagt
     5041 ggcggtagcc gatggtcagc cagactttgc gcgtgtccgc cagcgccgcg cgcggatcgc
     5101 ccgcgtggta atcggcgtac tccacgccgg cggacaaccg gggcgtgaaa gcccacgcga
     5161 acccgaagtc ccactccgtg ccgaggtccc attccctgaa gtcggcgcga aagcggtggt
     5221 actcgagatg cagcgtcgcc ttcgagacct ggacgccgat ctgcgcgcgc cggtcttcga
     5281 ggccgcgcgc cggggtcgtg gcgaagatgt cggcgcggcc ggtaaacagc tgcgtgctgc
     5341 ccagtggcgt ctgaaatccg tacagcccct cgttgctgcc gagcagctcc cgctcgatct
     5401 tgaggtagcc gcgcttcgcg gccacgccgg ctatcacacc ggcgcccacg cgccgatagg
     5461 gcgcgtcgat gcgcgggtcg ccgtcggcgt agtcgcgctg ctcggcgaac tctgcgctgt
     5521 acaacagctc cagcgcgccg gtcgccttcc acccgcccca caggcgcccg cccaggatct
     5581 ggttggaact gtcggcgaag ccggtcgccg agccggtgta gggctgatcc tgcagatagg
     5641 cgtagccggc gaccttgatg agcggccagg gctcgtagcg cgcgttcaga accacggtat
     5701 cggtatccgt ccgttcggcg aaggtgttgc gcacctgcgc gaaatagccg gcggtcagac
     5761 gcacctgcga caggctcgcg ttctccacca gcaggccgtc gaacgcctgc ggcagctgcg
     5821 cgaaatcgta gtcgccgacg aagcgctggt tgtcgatgcg caccagctgc cggccggcgc
     5881 ggatcagcgt gtgcggcacg ccgacgtagt ccaggtacag ccggttgaag tccgtggtcg
     5941 gctcgtcggc caccagcggg taatagccgg cgccgtacag ggcgacaacg ctgcccgggc
     6001 cgacgccagg cgtgtcgtcg tagtcgatcg cgccgctctc ggcgaagcgc gcgatgctga
     6061 tgccctcgat caccgcgctg aagccgcggt actccagggt cttccagccc agccgggtgc
     6121 gcaggctgcc ccagcgtgcc tcctcgggcc gcccgtcctg gtccacccag gtatagcgcg
     6181 ggcgcagctc gagcagcagc tcgccgtcgc gcagcgcctc gagcaacgag gccgcgacca
     6241 gcgggctgtc cagctcctgg gcgtgcaggg cgccgcacgc cgagcaaacc agcacagcgg
     6301 ccagcgacga tcggatccgg cgcatgtccc ctccgtgagc ggcgcgagcc gcgcgcggca
     6361 ctatagcgcg ggacgagcgg atccaggcgg tgggctatgc gctgcggtgc taggcactgt
     6421 gctggacgcc gcggcgatgg ccaccctgcg cgccgggcgc gaggctgccg gcctccaccg
     6481 cggcgatcgc cttggcctga ttcatgtcgt cggtataacc gcaggcatac aggcaacagg
     6541 cgagctggtt caccagcgga tacgggatcg gcgcctcccc ggccagcgcc aggcgcatcc
     6601 attgcgcggt ggcgtgcgcg tcgatgcccg caggcagacc cgcgaccggc ctgaccgggc
     6661 cggcttcctc ctcgaacagc gtctgccgca cgccacgccg gaagtgctcg atgcgcgggc
     6721 gccgccgcgg gctggcgaac ggctcgcctt cggtgctggc gagcagcagc gcgcaagcgc
     6781 ccagctccag cagcactccc gcgagctgtt ccagggtctg cgggcgagac gccccgatga
     6841 ccagcacact gtggccgccg aaggggtcga gaagcttcgc gaccagatgc gcgctgttgc
     6901 gcacgccgag gcggttgcgc agcgcgagca gcgccgccag cccgggacac agcaccgcgg
     6961 tgggcacgaa agtgatgtgc tccgcgtcca gcctcgcctg cgcctgcagc aggctggcgc
     7021 tgggcagcac accgaactcg cgcaggatgt agacgctcgc caggcgcccg ctgccttcca
     7081 gcgtcccgtg aaacagcacc ggaaccccca ggcgccggag cagcaggccg agcagcggca
     7141 gcaggttgtg ctcagtgcgt gcgccgccgt aagccggaag cagcaacggc cgggcccgcg
     7201 cggttggagc ccgcagcggg tacagccgct cgcgcagggc gcgctggaac ccgagcagct
     7261 cgccgccgga ttcggatttg aggtgcaacg cgatcagcac agcgcccagc tccagatcgg
     7321 gcaggccccc gtcgagcatc cccccgaaca gctcgtgcgc ctcgccctcc gcgagatcgc
     7381 gcacgctccc acccggttgg gcaatctgct tgatgacctg catcgtccgc atcgtcgtat
     7441 cccgcaagcg cagggccggc cactgcaacg cacgtgccac gcgtgccgca gccggctccg
     7501 gcccggcaat acagccgcct gcgtcgactc gcaccgcgaa tgccgccagc ccatggtgca
     7561 gatccagcag cccatgcctt ctgctggtgc acgaccgcgc gagcgaatgt gacaggagcg
     7621 gcctgtcggc aatcgaatac gctattcacg tgcggcgcaa cgcgctacca tgggtccgtt
     7681 gccggttggc accggcttcc ttgtgggagg ttcggatgaa ctcctggacc tgtctgcttg
     7741 ctgccgcggc tgcgttcctc ccgctgtccg cggcccacgc caagggcatc aggcttccgg
     7801 catcggcgga tcggcagttc gagacgctgc ggctggacag cgcgctcagc gacctgcgcg
     7861 caccggcaac ggcggctgcg cagggcgaga ccgatgcggt gttcgccgcc ggtcatgcgc
     7921 acccgctcga cgtgcagact caggaggtcg ccagcggcat gtaccggccg ctcgccgaga
     7981 acttgtccac gctgctggaa accagctacg cgcgcggcag cggcctgacc gccgagtggt
     8041 cggtgctcgg tcaggtcggc gcccggttcg gcgacgggtg gggtgtgcgg gcgggcgtgc
     8101 gccacagcga gcaggagctc gccaccatcc tgctgccgcc gacgctcgcg cctgcgacgg
     8161 ccgatctggg catgctcacc ttcgagcggc aatggaaccg ttaccgcggc gagtacacct
     8221 actacgccgg gcgcagcgat gccggcgcca gcgcgagcgg tcatcgcatc cagctgcact
     8281 actattactc accacgcagc tccgtgggcc tggcctacca caccagccgg cagctcgatg
     8341 ccggcggcga gctcggtacg ttcaacccgg cctccgaggt ggccaacgtg ggggtcgtgg
     8401 gcgaacactg gttctcgccg acctgggcgc tcaactacaa tgcgctggtc gaagacggcg
     8461 gcaaccaggg gctgaacccg gaactgcggc tggggctgcg cgtgcgcttt tgatctagta
     8521 atctagcagc gctccgacct gcccgccggc cctcgcgcgg tcgtcacgcg cgcgtcccgc
     8581 ctcgctgcaa gaacgatccg gggcgcaaga acaatctgga gaccgacctt gatttcgagc
     8641 acgtctggtc cgctggaccg tgcccgcgcc ctcgcgcgcc gggtcatgct gccttcctgt
     8701 ctcggcctcg ccgccgcctg tctcggtctc gccgcatgcg accgctccgc gcagcagggc
     8761 ccgccgcaga tgcccccacc cgaggtgagc gcggtcaccg tcgcgccgcg cgacatcgcg
     8821 atcacgctgg agtacgtcgg acgcaccgaa ggcgtgcgcg aggtcgaggt gcgcccgcgc
     8881 gtgagcggca tcctgcagcg ctggaattac accgagggaa gccacgtcaa ggccgggcag
     8941 agcctgttca ccatcgaccc cgcgccgttc caggccgtgg tggcgcgcgc cgaggccgag
     9001 ctggcgagcg ccagggcacg cgtggcgcag gcgcaacgcg atctcgagcg cttgaagccc
     9061 ctgctcgatc agggcatggt gagccgcaaa gcctacgacg acgccatcgc cgccgacgag
     9121 gtagcggtgg cgcagctgca aggcgccgag gctgcactgt cggaagccaa gctcgacctc
     9181 aaatacacgc gcgtggaagc gccgatcagc ggcgtcacca gccgcgccct caagtccgag
     9241 ggcagcctgg tcgaggcgca gaacacgctg ctcacctcca tctcgcagat cgacccgatc
     9301 cgcgtcatct tcagcatgtc cgaggccgag cgcttgaggt tcaacgagca ggcggccgcg
     9361 ggcaagctgc ggctgccgcg cgacaaccgc ttcgcggtca ccgtggtgct gcccgacggc
     9421 agcacctccg agcgttccgg cgtcgtggaa ttcagcgaca tccgcgtcga tccggccacc
     9481 gggaccaccg agttccgtgc cgtcatcccc aacccggacc accgcgtccg cccgggacag
     9541 ttcgtgcgcg tgaccctgcg cggggcgcaa cacgtgcagg cgctcgcggt accgcaacgg
     9601 gccgtgctgg agggcccgca gggcaagatc gtgctcgtgg tggacgacaa gggcatggcg
     9661 cagccgcgcc cggtgcaggt gggcgaatgg gccggtgagg actgggtcat cagcgacggg
     9721 ttgaagcccg gcgagcgggt gatcgtggac ggcgtggtga aggtccgccc ggggatgccg
     9781 gtgaagatcg ccgagacccc gcccgcaccc caggcggcgg cgcccgagca gcagcagccg
     9841 accgccaaac cttgagtccg ggaaccgcga ccatgcgtcg cggcgaagca tcgtccatca
     9901 gttccgcctc acgccaatga acgtctcgcg cttcttcatc gaccgcccga tcttcgccgc
     9961 agtgctgtcc gccttcctgg tgctcgccgg cctggcggcc atccgcgtgc tgccgatcgc
    10021 ccagtatccc gagatcgcgc cgccggtggt gaccgtgcgc gcgatctacc cgggggcctc
    10081 ggcccaggtg ctggagcaga cggtggccgc gccgctcgag aaccagatca acggcgtgga
    10141 gaacatgatc ttcatgagct cgaactcggg ctccaacggc ctgctcgaga tcctggtcac
    10201 cttcgatatc ggctcggacg ccgaccaggc ggcgctagac gtcaacaacc gagtcaagca
    10261 ggccgaggcg cgcctgccgg aggaggtgcg ccgccagggc gtcacggtgg agaaaggctc
    10321 ctcggcgttc ctgatcgtgt acggcttcta ctcccccgat gggcgcttcg acgacctgta
    10381 caccagcaac tacgtcacgc tcaacgtgct ggacgcgatc aagcgcgtgc cgggcaccac
    10441 caacgtccag attttcggcg ccaaggacta cgccatgcgc atctgggtgc tgcccgagcg
    10501 gctgacccag ctgcaactga ccaccgacga cctggtgcgc gccatcaccg agcagaacgc
    10561 ccagttcgcc gccggcaaga tcggacagac gccgagcggc ggtccacagg agctggtgta
    10621 caccatcacc acccgcggcc gcatgtcgga ggtgagcgag ttcgagaaca tcatcgtgcg
    10681 cgctctgccg ggcggggcca aggtgcggtt gaaggacgtg gctcgcgtcg agctgggctc
    10741 gaaggactac gagttcatcg gccgggtgaa cggcaagcag gccacgctga tgggcatctt
    10801 cctgcagccg ggcgccaacg ccctggacgt ggccgaggaa tgcgacgcgg tcatggcgca
    10861 gctcgcgaag cgcttccccg agggcctcac ctacgccaac gtgtacgaca ccacgcgctt
    10921 cgtgaaggtg tcgatccgcg aggtggtcaa gactctggcc gaagccatgg cgctggtgtt
    10981 cctggtcgtg ttcgtgttcc tgcagaactg gcgcgccacg ctgattccgt tcgccgcggt
    11041 gccggtgtcg ctgatcggca ccttcgccgg gctgcagctg atgggctatt cgatcaacac
    11101 gctgaccctg ttcggcatgg tgctttcgat cggcatcgtg gtggacgacg ccatcgtggt
    11161 gctggagaac gtcgagcgca tcatgcacga gcagcaccgc gatgcgcgcg aggcggcggt
    11221 ccaggcgatg cacgaggtca ccggcccggt ggtggccatc gtgctcacgc tgtgcgcggt
    11281 gttcgtgccg atcgctttcc tgggcggact caccggcgag ctgtatcgcc agttcgcggt
    11341 caccatctcc atcgccgtgg tgatctccgg cttcgtcgcg ctcaccctga cgccttcgct
    11401 ctgcgtgctg atcctcaagc gccgccaccg cccgcccggc ccgttcttcc gctggttcaa
    11461 cgaggctttc cggcgggtga ccgcgcgcta ctccgacggg gtgggctgga tgctgcggcg
    11521 gggcgccatc ggcatggcgc tgttcgccgg catggtggcg atcacggctt ggctgtggcg
    11581 cagcacgccc accagcctgg tgcccgacga ggacaccggc tactacatcg gcgccgtgtt
    11641 cctgcccgac ggcgcgacgc tgcagcgcac cgacgcggtg gtgggcaagg tcaccgaggc
    11701 gatgctgtcc aacccggcca accagtacgc cgttgccttc accggcctgg atttcctcgg
    11761 cggcggtttc cgcaacagcg cggccaccat cttcgcctcg catcggcact gggacgaccg
    11821 gcccggggta tacacaccgc agctggtggg cgagttcttc gcgaagaccg cgcacatcaa
    11881 ggaagggttg gtgctcgcct tcgcgccgcc cccgatcttc gggctgggca acgccggcgg
    11941 tttcgagttc tacatccaga accgcggcga aggcgggccg cagcgcctgg ccgaggtgat
    12001 ggggcagttt ctcgcgcgcg ccaacgccga ccccatgctg ggcgcgggcg gcggctgggt
    12061 gcagacgcta tggcggccga acgtgccgca gctgttcgtc gacttggatc gggaaaaggc
    12121 caaggcgctg ggcgtgccga tcaatgccct gtacagctcg atcgccgcca cgctgggcac
    12181 ctactacgtc aacgacttca acaagttcgg ccgcacctgg caggtgctga tgtcggccga
    12241 cccggtgtac cgcaaacgcc ccgactcgat cggcgagatc tacgtgcgct cggagcacgg
    12301 cgagatgatc ccgctgaagg cgctcgccga agtgcgctac tcctcgggcc ccgactcgct
    12361 ggaccgcttc aacaacctgc cggcggtgaa gctgctcggt cagggcgcgc ccggagtcag
    12421 ctccgggcag gcgatccagg cggtagagcg catcgccgaa gaggtgctgc ccgccgagtt
    12481 cagctacgac tggggcggcg cctcgttcca ggagaagcgc tccgccggca cctcggccat
    12541 cgcgctcggt ctggcggtga tcatggtgtt cttgatcctc gccgcgcagt acgagcgctg
    12601 gtcgctgccg ttctcggtgc tgatggcgct gcccttcggc accttcggcg cgctggctgc
    12661 catctggatc aacaacctca ccctgccctt caccggccgc acgccgctcg ccaacgacgt
    12721 gtacttccag atcggcctgg tcacgctgct cgggctggcg gcgaagaatg ccatcctgat
    12781 cgtggagtac gccgtgctga agaagcagga gggtctgtcc acctgcggct cggcgctgga
    12841 ggcggcgcgc ctgcgcttcc ggccgatcct gatgacctcg ctcgccttca tcctgggggt
    12901 cgccccgctg gcgttctcga ccggcgcggg tgcgggggcg cgcctggcgg tgggcaccgg
    12961 cgtcatgggc ggcatgctcg ccgccacgtt cctggcgatc ttctttatcc cgttgttctt
    13021 caagctgatg accgacgggc atctacgcga gaggcgcagc accggcgaga tccgcacgga
    13081 gatcgagcac caccgggccc tcggggagca ccccatcgac cggcccggcc accatccgcc
    13141 cgccctggga acggcctgag gtcccctcac ctaccctctg atcgggctgg cactgccagt
    13201 tgcctggggg tgtccttgct cacacttgcg agcagcgcct caaaggacgt ctcgccgccg
    13261 tgacgcgcca ggtccggtta gcgggcgcgt gcggcgggtc gcgtcggtgg tccacggcag
    13321 tcacgacctt ctcgctgcgt gatggctgct cttgacatgg gtaaatttcc cacatatttt
    13381 acgactaaag ggaaaaattc ccacatcagc tcctggattc gacagccagg caaaaggggc
    13441 gggcctgcaa caagatttgc ctcggtaatc cctttgaggt catgacgttg tacgctggtt
    13501 ttgtaaggaa tccgcgggta cactgaggct tggacaccgg gggaggccgg gggccatgac
    13561 ggcgcaccaa atactgggtt ggcagcaaca ggccgagccc gaagtcgggt tcgccatgga
    13621 ccgcttcggc aacatcagcc agatgggagc cggctggagc atgctgaccg gcggctcgcc
    13681 gcatgacgtg ctgtcccggc ccttcgccga catcctccat ccggcg
//
LOCUS       DASPGE010000365         4412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_329722, whole genome shotgun sequence.
ACCESSION   DASPGE010000365 DASPGE010000000
VERSION     DASPGE010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329722"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VD791_11815"
     CDS             complement(<1..234)
                     /locus_tag="VD791_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HXV08711.1"
                     /translation="MIPTLALVGRPNVGKSTLFNRLTRSRDALVADIPGLTRDRHYGR
                     GRLGNRSFLAVDTGGLEPRARSGIFGAMARQTLQ"
     gene            complement(343..1488)
                     /gene="bamB"
                     /locus_tag="VD791_11820"
     CDS             complement(343..1488)
                     /gene="bamB"
                     /locus_tag="VD791_11820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HXV08712.1"
                     /translation="MTILALRSGGRAAVLALLGAGLAACGGADAVKPAELVDFKPLAK
                     ARVAWRASVGEAKPFVFAPAFYDGSVYAASRAGLLARLDGASGKPIWRVDTKQKLSGG
                     VGADADLVLVGSPKGVVLAYSTDGKELWRAQVSSEVLGAPRAAEGMVVVRSADGRFFG
                     LDAATGARKWEHQITLPPLLLRAHPSVTIVRQLVLAGLPAGRVLALDLASGTPVWEAL
                     VAQPKGDNELERITDIAAAPVLVSDDQACAAAYQGRVACLDIGKGTFVWGRDASSAGR
                     LGVDSLTVFVTEPNGTILAFDKESGATVWKQDKLLNRGVTGPTVTGDFVTVGDYQGYV
                     HVLDAEEGAFAARIATDGSPIGAEPLRVGDNIVVQTRKGGVFAIAIK"
     gene            complement(1500..2138)
                     /locus_tag="VD791_11825"
     CDS             complement(1500..2138)
                     /locus_tag="VD791_11825"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV08713.1"
                     /translation="MAYDLEEQEQIDALKAWWKQNARLVSTAVLAAALAAASVSGWRW
                     YQQRQSEQASALYGALEQAVGAGDAQQARQLSAQLMERYPRTAYGPMAALVAAKISFD
                     AGDLKSAGTQLEWATEHARDEETAAVAHLRLAAVRLDERRYDEALAALGEKHPESFDG
                     LYADLRGDVLVAQGKIEEARAAYRQALEKLPPGSGFRRMVQVKLDGIGGGGT"
     gene            complement(2150..3457)
                     /gene="hisS"
                     /locus_tag="VD791_11830"
     CDS             complement(2150..3457)
                     /gene="hisS"
                     /locus_tag="VD791_11830"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HXV08714.1"
                     /translation="MSDTIQAIRGMSDVLPDQAPSWEWFEDTVRDWLCDYGYRNMRMP
                     IVEKTELFVRSIGEVTDIVEKEMYTFVDHLNGESLTLRPEGTASCVRAALQHNLLYAG
                     PQRVYYSGPMFRHERPQKGRYRQFHQVGVEALGLPGPDVDAEHMAMCARLWRRLKLDG
                     IRLEINTLGGPESRARYRARLVKYLESRFDELDEDGKRRVHANPLRVLDSKNPALQPV
                     IEAAPRLYDDLDEDSLKHFEGLQAILRALDIDYQIRPRLVRGLDYYNHTVFEWTTERL
                     GAQGAVCAGGRYDGLIAQLGGKPAPGCGFAMGVERLLALLSEGALPPAAPADAYLAYS
                     GAGVELHAWRMAEGLRDAGLAVILHCGGGSFKSQLRKADASGARFAVIVGEAEAAAGR
                     VAVKPLRELGEQVTVSLAGAVEMLQAGRAPGFERQEKGGAGKP"
     gene            complement(3486..>4412)
                     /locus_tag="VD791_11835"
     CDS             complement(3486..>4412)
                     /locus_tag="VD791_11835"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HXV08715.1"
                     /translation="NPGNVGRGVKRDEQFAVMIEAACRYGKPVRIGVNWGSLDQELLA
                     RLMDENARRATPLDAGSVMREALVRSALESAQRAEQLGLPGDRIVLSCKVSGVQDLIR
                     VYRELAGRCDYPLHLGLTEAGMGSKGIVASAAAMAVLLQEGIGDTIRVSLTPEPGGER
                     EREVRVAQELLQTMGLRSFAPMVVACPGCGRTTSTTFQELADSIQGWLRAQMPAWKTT
                     YPGVERMTVAVMGCVVNGPGESRQADIGISLPGSGEQPVAPVYVDGVKTVTLKGATIA
                     EDFQKIVSEYVAARYGAGNAPGTRAASADAAA"
BASE COUNT          596 a         1676 c         1468 g          672 t
ORIGIN      
        1 ctgcagggtc tgcctggcca tcgcgccgaa gatgccgctg cgcgcgcgcg gctcgagccc
       61 gccggtgtcc accgccagga agctgcggtt gccgagccgc ccgcgcccgt agtgccggtc
      121 gcgcgtgagc ccgggaatgt cggccaccag cgcatcgcgg ctgcgcgtga ggcggttgaa
      181 cagcgtcgac ttgccgacat tgggccggcc gacgagcgcg agtgttggga tcactggttt
      241 ctaaccacag aggcacagag acacagagga acacacagac accgtacttc ctcgcgcgct
      301 acaggtcgca gttttctgtg cctctgtgcc tctgtggtga agtcacttga tggcgatcgc
      361 gaacacgcca cccttgcgcg tctgcaccac gatgttgtcg ccgacgcgca gcggctcggc
      421 gccgatcggg ctgccgtcgg tggcgatgcg cgcggcgaac gcgccttcct cggcgtcgag
      481 gacgtgcacg tatccctggt agtcgccgac cgtcacgaaa tcgccggtca ccgtaggccc
      541 ggtgaccccg cggttgagca gcttgtcctg cttccagacg gtggcgcccg actccttgtc
      601 gaacgccagg atggtgccgt tcggctcggt gacgaacacg gtgagcgagt cgacccccag
      661 gcgcccggcg gacgaggcat cccgccccca gacaaacgtc cccttgccga tgtccaggca
      721 ggcgacgcgg ccctgatagg ccgcggcgca tgcctgatcg tcgctcacca gcaccggggc
      781 tgcggcgatg tcggtgatgc gctccagctc gttgtcgccc ttgggctgtg ccaccagcgc
      841 ttcccagacc ggcgtgccgc tggcgagatc gagcgcgagc acgcgcccgg ccggcagtcc
      901 cgcgagcacc agctgacgca cgatggtcac gctcgggtgc gcgcgcagca gcagcggcgg
      961 cagggtgatc tgatgttccc acttgcgcgc gccggtggcc gcgtccagtc cgaaaaaacg
     1021 cccgtcggcg ctgcgcacca cgaccatccc ttccgccgcg cgcggcgcgc ccagcacttc
     1081 gctgctcacc tgcgcgcgcc acagctcctt gccgtccgtg ctgtacgcca gcacgacccc
     1141 cttggggctg cccaccagca ccaggtcggc gtccgcgccc acgccgccgg agagcttctg
     1201 cttggtgtcc acgcgccaga tcggcttgcc gctggcgccg tccaggcgcg ccaggagccc
     1261 ggcgcgggag gcggcgtaga ccgagccgtc gtagaacgcc ggcgcgaaca cgaaaggctt
     1321 ggcctcgccc accgaggcgc gccacgccac gcgtgccttc gcgagcggct tgaagtcgac
     1381 cagctcggcg ggcttgacgg catccgctcc cccgcacgcg gcgagccccg cgccgaggag
     1441 cgcgagcacg gcggcccgcc cgccggaacg cagcgccagg atggtcatgc gcggccgttt
     1501 caagtgccgc cgccgccgat gccgtcgagc ttgacctgca ccatgcgccg aaagccgctg
     1561 ccgggcggca gcttctccag cgcctgccgg taggccgcac gggcctcttc gatcttgccc
     1621 tgcgccacca gaacgtcgcc gcgcaggtcc gcgtacagac cgtcgaagga ttccggatgc
     1681 ttctcgccga gcgcagcgag cgcctcgtcg tagcgccgct cgtccaggcg cacggccgcc
     1741 agccgcaggt gcgccaccgc cgcggtctcc tcgtcgcgcg cgtgctccgt cgcccattcg
     1801 agctgggtgc cggcgctctt caggtcgccc gcgtcgaaac tgatcttggc cgccaccagc
     1861 gccgccatcg gcccgtaagc ggtgcgtgga tagcgttcca tgagctgcgc ggaaagctgg
     1921 cgcgcctgct gcgcgtcgcc ggcacccacc gcttgctcca gggccccgta cagcgccgag
     1981 gcctgctcgc tctgccgctg ctggtaccag cgccagccgc tcacgctggc cgcggccagc
     2041 gccgcggcaa gcacggcggt gctcaccagc cgcgcgttct gcttccacca ggccttgagc
     2101 gcgtcgatct gctcctgctc ttccaggtcg tatgccatcg ttgtcggtcc tatggtttgc
     2161 ccgcgccgcc tttctcttgc cgttcgaacc cgggcgcgcg cccggcctgc agcatctcca
     2221 cggcgccggc cagagacacg gtcacctgct cgccgagctc gcgcagcggc ttcaccgcca
     2281 cccgcccggc cgccgcctcc gcctcgccga cgatcacggc gaagcgcgcg ccgctcgcgt
     2341 ccgccttgcg cagctgcgac ttgaagctgc cgccgccgca gtgcaggatc accgccagcc
     2401 cggcatcgcg caggccctcg gccatgcgcc aggcgtgcag ctcgacgccc gcaccgctgt
     2461 aggcgaggta ggcgtccgcg ggcgcggccg gcgggagcgc gccttccgag agcagcgcga
     2521 gcagccgctc cacccccatg gcgaagccgc atccgggcgc aggtttgccc cctagctgtg
     2581 cgatgaggcc gtcgtagcgg ccgccggcgc acaccgcgcc ctgggcgccg agacgctcgg
     2641 tggtccactc gaacaccgtg tgattgtaat agtccaggcc gcgcaccagc cgcggacgga
     2701 tctggtagtc gatgtcgagc gcgcgcagga tcgcctgcag cccctcgaag tgcttgagcg
     2761 aatcctcgtc gagatcgtcg tacagccgcg gcgcggcctc gatcaccggc tgcagcgccg
     2821 gattcttgct gtccagcacg cgcagcggat tggcgtgcac gcgccgcttg ccgtcctcgt
     2881 ccagttcgtc gaagcgtgat tccaggtact tcaccagccg cgcccggtag cgcgcccgcg
     2941 actccgggcc gccgagcgtg ttgatctcca ggcggatgcc gtcgagcttc agccgccgcc
     3001 acagccgcgc gcacattgcc atgtgctccg cgtccacgtc aggccccggc agccccagcg
     3061 cttccacgcc gacttggtgg aactggcggt agcggccctt ctgcggccgc tcgtgccgga
     3121 acatggggcc gctgtagtac acgcgttgcg gcccggcgta cagcagattg tgctgcagcg
     3181 ctgcgcgcac gcaggaggcg gtgccctcgg gccgcagcgt caggctctcg ccgttgaggt
     3241 gatcgacgaa ggtgtacatc tccttctcga cgatgtcggt cacctcgccg atcgagcgca
     3301 cgaacagctc ggtcttctcg acgatcggca tgcgcatgtt gcggtagccg tagtcgcaga
     3361 gccaatcgcg caccgtgtcc tcgaaccact cccacgacgg ggcctggtcc gggagcacgt
     3421 cgctcatgcc ccggatcgcc tggatggtgt cgctcatgct gctgaagccg gcgggcgggc
     3481 gcccgtcacg cggccgcgtc ggcgctcgcc gcgcgcgtgc ccggagcatt cccggcgccg
     3541 tagcgcgcgg ccacgtactc gctgacgatt ttctggaagt cctcggcgat ggtggcgccc
     3601 ttcagggtga cggtcttcac gccgtccacg tacaccggcg ccaccggctg ctcgccggaa
     3661 cccggcaggc tgatgccgat gtccgcctgc cggctctcac ccggcccgtt caccacgcag
     3721 cccatcaccg cgacggtcat gcgctccaca cccggatagg tggtcttcca agccggcatc
     3781 tgcgcccgca gccagccctg gatgctgtcg gccagctcct ggaaggtggt gctggtggtg
     3841 cgcccgcagc ccgggcaggc caccaccatc ggcgcgaacg agcgcagccc catggtctgc
     3901 agcagctcct gcgcgacgcg gacctcgcgc tcgcgctcgc ccccgggctc gggcgtgagc
     3961 gagacgcgga tggtgtcgcc gatgccctcc tgcagcagca ccgccatggc ggcggcggaa
     4021 gccacgatgc ccttgctgcc catgccggcc tcggtcaggc ccaggtgcag cgggtagtcg
     4081 cagcggccgg cgagctcgcg atacacacgg atcaggtcct gcacgccgct cactttgcac
     4141 gacagcacga tgcggtcgcc cggcagaccg agttgctccg cgcgctgcgc gctctccagc
     4201 gccgagcgca ccagcgcttc gcgcatcacg ctgccggcgt cgagcggcgt cgcgcgccgc
     4261 gcgttctcgt ccatcaggcg cgcgagcagc tcctggtcca ggctgcccca gttgacgccg
     4321 atgcgcaccg gcttgccgta gcggcaggcg gcctcgatca tcacggcgaa ctgctcgtcg
     4381 cgcttgaccc cgcgcccgac gttgccggga tt
//
LOCUS       DASPGE010000366         4502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_212341, whole genome shotgun sequence.
ACCESSION   DASPGE010000366 DASPGE010000000
VERSION     DASPGE010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212341"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /gene="era"
                     /locus_tag="VD791_11840"
     CDS             complement(<1..732)
                     /gene="era"
                     /locus_tag="VD791_11840"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HXV08716.1"
                     /translation="MGNTPHRAGTIAIVGRPNVGKSTLLNRLVGQKVSITSRRPQTTR
                     HRVVGIVTRPEAQLVFVDTPGMGTGHGTELGRRMNRAAAQALAEVDVALVLVEALRFG
                     AQDRQVLGLLPVGMPAVAAVNKIDLLKDKAALLPYLANLSAAFPFRAIVPVSAKTGRQ
                     TGELLDELAALLPEAPPLYAADDITDRSERFLAAELLREKLFRLLGAELPYAAAVEIE
                     RFETEGGLRRIAAAVLVDRPGHKAIV"
     gene            complement(736..1404)
                     /gene="rnc"
                     /locus_tag="VD791_11845"
                     /pseudo
     CDS             complement(736..1404)
                     /gene="rnc"
                     /locus_tag="VD791_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851961.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
     gene            complement(1421..1780)
                     /locus_tag="VD791_11850"
     CDS             complement(1421..1780)
                     /locus_tag="VD791_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HXV08717.1"
                     /translation="MRTQRGLSLIGLLLVSAVVVMVALIGFKVLPAYIEYLAIKKAVT
                     EIAYAPETRGGQANEVRKAFDRRATIDNITSITGQDLEVSKMGDGFEVIASYSVQVPL
                     FGNVSACIDFIAEGGRR"
     gene            complement(1815..2732)
                     /gene="lepB"
                     /locus_tag="VD791_11855"
     CDS             complement(1815..2732)
                     /gene="lepB"
                     /locus_tag="VD791_11855"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXV08718.1"
                     /translation="MNFALAMLLLLVLTGGIWLLDRFALARRRQAAVRAALLQAESAL
                     ALKPGEGQADYQTRKDQILGEIHQKVGREPWYVEYSKSFFPVILLVFLLRSFLVEPFK
                     IPSGSMIPTLLVGDFILVNKYIYGIRLPVVNVKVLSISRPRRGEVMVFRFPENPSLDY
                     IKRVVGEPGDTVAYRDKQLYVNGEAVPTTPESDYQYAEGGLNYIKTKRLRETLGQHNH
                     AVLINPESPPMQVAGVRNFEHRENCDYSESGFECKVPAGHYFLMGDNRDSSSDSRYWG
                     FVPERNIVGKAFMIWWNFSDFGRIGTRIN"
     gene            complement(2955..>4502)
                     /gene="lepA"
                     /locus_tag="VD791_11860"
     CDS             complement(2955..>4502)
                     /gene="lepA"
                     /locus_tag="VD791_11860"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HXV08719.1"
                     /translation="DFSYEVSRSLAACEGALLVVDASQGVEAQTVANCYTAIEQGVEV
                     VPVLNKIDLPSADPVRVISEIEDIIGIPATDAIRCSAKTGEGVGDVIEAVVRRIPPPG
                     GDPDAALKALIIDSWFDNYVGVVMLVRVVDGSLAPKDKILLMSSQASYLCEQVGVFTP
                     KSVPKETLAAGEVGFVVAGIRELGNAQVGDTITLANRRAAAPLPGFKQIKPQVFAGLY
                     PVEANEYGALRDALEKLRLNDASLRFEPESSQALGFGFRCGFLGLLHLDIVQERLERE
                     YDMHLLITAPTVVYQVQLADGSVLEVENPSRLPDASRIEEIREPIITASILVPSDYVG
                     PVMTLANGKRGAQKNLQYLGRQVMLTYELPLNEVVMDFFDKLKSVSRGYASLDYDFKE
                     FRAADLVRLDILINGERVDALSLIVHRASAQPRGRELVEKMRQLIPRQMFDVAVQAAI
                     GSHIIARETIKALRKNVLAKCYGGDVTRKRKLLEKQKAGKKRMKQIGTVEIPQEAFLA
                     ILQVEGK"
BASE COUNT          692 a         1580 c         1461 g          769 t
ORIGIN      
        1 cacgatcgcc ttgtgcccgg gccggtccac cagcaccgcc gccgcgatgc gccgcaggcc
       61 gccctcggtt tcgaagcgct cgatctccac cgcggcggca tagggcaact ccgcgcccag
      121 cagccggaac agcttttcgc gcagcagctc ggccgccagg aagcgttcgc tgcggtcggt
      181 gatgtcgtcc gcggcgtaga gcggcggcgc ttcgggcagc agcgccgcca gctcgtccaa
      241 cagctcgccg gtctgccgcc cggtcttggc gctgaccggg acgatggcgc ggaacgggaa
      301 ggccgccgac aagttggcca gataaggcag cagcgccgcc ttgtccttca gcagatcgat
      361 cttgttcacc gccgccaccg ccggcatccc caccggcagc agcccgagca cctgccggtc
      421 ctgggcgccg aagcgcagcg cctccaccaa caccagcgcc acgtcgacct cggcgagggc
      481 ctgcgccgcc gcgcggttca tgcgccggcc gagctccgtg ccgtgaccgg tccccatccc
      541 cggcgtgtcc acgaacacca gctgggcctc gggtcgggtg acgatgccga ccacccggtg
      601 gcgggtggtc tgcggccggc gcgacgtgat gctgaccttc tgccccacca agcgattgag
      661 cagggtggat ttgccgacgt tgggccgccc gacgatggcg atggtgccgg cgcggtgcgg
      721 cgtgttgccc atctagggcc ctacggcgct gtcgtaggcg gcgcgggcgg cgctctgctc
      781 ggcgctgcgc cggctcggcc cttcgccctc ggtgcggatg cccagttcgg ggatcaggca
      841 ttccacgtgg aagtgctgct cgtgcgcctc gccgcgcacg ctcaccacgt tgtagcgcgg
      901 cagcggcagg tggcgcgcct gcagcagctc ctgcagcagg gtcttcgggt ccttgccgtg
      961 ggtgctggga tcgacctccg ccaggagcgg ctggtacagg cgctccacca cggccgcggc
     1021 ggcctcgaag cggccgtcca ggaacaccgc cccgagcacc gcctccaggg cgtcggcgag
     1081 gatcgaaggc cggcggaagc cgccgctctt cagctcgccc tcgcccagca gcaggtgctc
     1141 gcccaggttc aggcccgccg ccaggcgcga caggctgtcc tggttcacca ggttggcgcg
     1201 cagccgcgac agatggcctt cgggcagccc cgggaagcgg tggtagagca gcgaggcgat
     1261 cacgcagttg agcacgccgt cgccgaggaa ctcgaggcgc tcgttgtgcg cggcactgtg
     1321 gctgcgatgg gtgagcgcct gccgcagcag gcgcgggtcc tggaagcggt aacccagccg
     1381 ggcgagcagg tcctcttcgc tcatgctccc tggcggcgct tcatctcctc ccgccctcgg
     1441 cgatgaagtc gatgcaggcg ctgacgttgc cgaacagcgg gacctgcacc gagtagctgg
     1501 cgatgacctc gaagccgtct cccatcttgc tcacctcgag gtcctggccg gtgatcgacg
     1561 tgatgttgtc gatcgtggcg cggcggtcga aagccttccg cacttcgttc gcctgcccgc
     1621 cgcgcgtctc cggcgcgtag gcgatctcgg tcacggcttt cttgatcgcc agatactcga
     1681 tgtaggccgg cagcaccttg aagccgatga gcgccaccat caccaccact gctgacacca
     1741 gcagcaatcc gatcaggctc aatccccgtt gcgttcgcat cgccttctcc gcccaatgcc
     1801 gtttcaaagt tatcctagtt gatacgcgtc ccgatacggc cgaaatcgct gaagttccac
     1861 cagatcatga aggccttgcc gacgatgttg cgctccggca cgaagcccca gtagcgactg
     1921 tcgctgctgc tgtcgcggtt gtcgcccatc aggaagtaat gcccggccgg caccttgcac
     1981 tcgaatcccg attcgctgta atcgcagttt tcgcggtgct cgaaattgcg cacgcccgcc
     2041 acctgcatcg gcggcgactc cgggttgatc agcacggcgt ggttgtgctg ccccagcgtc
     2101 tcgcgcaggc gcttggtctt gatgtagttc aagccgcctt ccgcgtactg gtaatcggac
     2161 tccggcgtcg tcggcaccgc ctcgccgttc acatacagct gcttgtcgcg gtaggccacg
     2221 gtgtcgcccg gttcgcccac cacgcgcttg atgtagtcga gcgacgggtt ctcggggaag
     2281 cggaacacca tcacctcccc gcgccgcggc cggctgatgc tcagcacctt cacgttgacc
     2341 accggcaacc gaattccata gatgtacttg ttgaccagga tgaagtcgcc caccagcagg
     2401 gtcggaatca tcgagccgga cgggatcttg aacggctcca ccaagaagga gcgcagcaga
     2461 aacaccagca ggatcaccgg gaagaagctt ttcgagtact ccacgtacca gggctcgcgc
     2521 cccacttttt ggtggatctc cccgaggatc tggtccttcc tggtctgata atcggcctgc
     2581 ccctcgccgg gctttagggc gagcgcggac tccgcctgca gcagcgccgc gcgcaccgcc
     2641 gcctgccggc gccgcgccag ggcgaagcgg tccagcagcc agatgccgcc ggtcagcacc
     2701 agcagcagga gcattgcgag ggcgaaattc atgggggcgg tctagcctgg actattcatg
     2761 aggatcgcgc gctgatggcc gcctacacca caccgccccg gcgcctgcgg cgccaccccg
     2821 cctcgtgaga ggcggggagc agtagattcc cctcctcttc gaggaggggt ggcacgcgaa
     2881 gcgtgacggg gtggtacaag aagcgccgcc ttttctcgcg ttcatgaatt atccaggctg
     2941 gtggcgtgcg cgggctactt gccctcgacc tggaggatgg ccaggaaggc ctcctggggg
     3001 atctcgaccg tgccgatctg cttcatgcgc ttcttgcccg ccttctgctt ctccagcagc
     3061 ttgcgcttgc gggtgacgtc gcctccatag catttggcca gcacgttctt gcgcagcgcc
     3121 ttgatcgtct cgcgcgcgat gatgtgcgag ccgatcgccg cctgcaccgc gacgtcgaac
     3181 atctgccgcg ggatcagctg gcgcatcttc tccaccagct cgcgcccgcg cggctgggcg
     3241 ctggcgcgat gcacgatcag cgacagcgcg tccacgcgct cgccgttgat caggatgtcg
     3301 agcctgacca gatcggcggc gcgaaactcc ttgaagtcgt agtcgagcga ggcgtagccg
     3361 cggctcaccg acttcaactt gtcgaagaag tccatcacca cttcgttgag cggcagctcg
     3421 taggtgagca tcacctgccg gcccaggtat tgcaggttct tctgcgcgcc gcgcttgccg
     3481 ttggcgagcg tcatcaccgg gccgacgtag tcgctcggca ccaggatgct ggcggtgatg
     3541 atcggctcgc ggatctcttc gatccgggag gcgtccggaa gccgcgaagg attctccacc
     3601 tcgagcacgc tgccgtcggc gagctgcacc tggtagacca cggtgggcgc ggtgatcagc
     3661 aggtgcatat cgtactcgcg ctccaggcgc tcctgcacga tgtccaggtg cagtagcccc
     3721 aggaagccgc agcggaagcc gaaacccagc gcctgcgagc tctccggctc gaagcgcagc
     3781 gacgcgtcgt tgagccgcag cttctccagc gcatcgcgca gcgccccgta ctcgttcgcc
     3841 tccaccgggt agagcccggc gaacacctgc ggcttgatct gcttgaagcc gggcagcggt
     3901 gcggccgcgc ggcggttggc gagcgtgatg gtgtcgccca cctgggcgtt gcccagttcc
     3961 ctgatgccgg ccaccacgaa gcccacctcg ccggcggcta gcgtctcctt cggcaccgac
     4021 ttgggggtga acacgcccac ctgctcgcac agatagctcg cctgcgagga catcagcagg
     4081 atcttgtcct tcggcgcgag gctgccgtcc accacgcgca ccagcatcac cacgccgacg
     4141 tagttgtcga accaggagtc gatgatcagc gctttgagcg ccgcatcggg gtcgcccccg
     4201 gggggcggga tgcgccgcac gacggcttcg atgacgtccc cgaccccttc cccggtcttg
     4261 gcgctgcagc ggatcgcgtc ggtcgccggg atgccgatga tgtcctcgat ctcggagatc
     4321 acgcgcaccg ggtcggccga gggcagatcg atcttgttca gcaccggcac cacttccacg
     4381 ccctgctcga tcgccgtgta gcagttggcc acggtctgcg cctccacgcc ctgggaggcg
     4441 tccaccacca gcagcgcgcc ctcgcacgcg gcgagcgagc gcgacacctc gtaggagaaa
     4501 tc
//
LOCUS       DASPGE010000367         7705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1735243, whole genome shotgun sequence.
ACCESSION   DASPGE010000367 DASPGE010000000
VERSION     DASPGE010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7705
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735243"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VD791_11865"
     CDS             <1..836
                     /locus_tag="VD791_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 - glycopeptide
                     alpha-N-acetylgalactosaminidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase 100 family protein"
                     /protein_id="HXV08720.1"
                     /translation="LIAVDFLHRCAAFGDLRRRLASPIARALDWLACQEHQRFFLLMQ
                     GEASDWADIMPRSGFVLYSNALWYRVKRLYRLPRADETRRYFNHLFHPFSAGLAEYRR
                     ARLLTDYVLRKARNRDLYLSFVNFSFFGEEGDVFGNLLAILMGLTSARASRRVLRALL
                     EARAHRPYPVRAVCDPITRADPLWRDYMQRHRQNLTWQYHNGGVWPFVGGFWVCALAQ
                     SGESALARAELHNLARANALGGWAFSEWLHGRTAAPRGMPGQSWNAAMYLLACSAVAR
                     M"
     gene            complement(841..1593)
                     /locus_tag="VD791_11870"
     CDS             complement(841..1593)
                     /locus_tag="VD791_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXV08721.1"
                     /translation="MKLHILSDIHLEFAPFEPPATDADVVLLAGDIWVGARGVEWAAR
                     TFDRPVIYVLGNHEYYGGHFKNTLTKMKRVAAGTCVHVLDGETLVLDGVRFLAATLWT
                     DFAVTGNPVLAQAEAQHNMSDYRHIRAAAWRRLRPSDTHADHLAARMFLQAALREPFD
                     GRTVVVTHHGPARASIAAPYRNGYSHINASYVSDLEVLLGPPVLLWVHGHTHYSFDYR
                     LGGTRVVCNPRGYLPYEPNERFDPGMVVELKC"
     gene            complement(1940..2953)
                     /gene="ilvC"
                     /locus_tag="VD791_11875"
     CDS             complement(1940..2953)
                     /gene="ilvC"
                     /locus_tag="VD791_11875"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HXV08722.1"
                     /translation="MKVYYDKDADLSLIKGKTIAVIGYGSQGHAHALNLHESGVKVVV
                     GLRKGGSWTKAVNAGLTVKEVPDAVKDADFVMMLMPDEHIAATYRKEVEPNMKRNATL
                     GFAHGFNIHFGQVIPREDIDCVMIAPKAPGHTVRSTYVQGGGTPCLIAVAQDKSGKAR
                     DLALSYAAAIGGARAGVIETNFREETETDLFGEQVVLCGGTTALVQAGFEVLVEAGYA
                     PEMAYFECLHELKLIVDLMYEGGIANMRYSISNNAEYGDFTRGPRIITEDTKNEMRRI
                     LKEIQTGAYAQEFLLENQTGATKLNALRRIGREHEIEIVGEKLRAMMPWLKKDRLVDR
                     SKN"
     gene            complement(2964..3455)
                     /gene="ilvN"
                     /locus_tag="VD791_11880"
     CDS             complement(2964..3455)
                     /gene="ilvN"
                     /locus_tag="VD791_11880"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HXV08723.1"
                     /translation="MRHIISLLLENESGALSRVSGLFSARGYNIESLTVAPTEDSTLS
                     RMTIVTSGSDDVIEQITKQLNKLVDVVKVVDLSDGEHIERELMLVKVRAAGKDREEMK
                     RMAEIFRGRIIDVTDKSYTIELTGDTGKLDAFLNALERGSILETVRTGASGIGRGDRV
                     LRI"
     gene            complement(3589..5319)
                     /locus_tag="VD791_11885"
     CDS             complement(3589..5319)
                     /locus_tag="VD791_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HXV08724.1"
                     /translation="MEMTAPGVTSQVELTGAEIAIRCLQEEGVQFVFGYPGGAILPIY
                     DEMFKQDKVKHILVRHEQAAVHGADGYSRATDKVGVVMVTSGPGLTNAVTGIATAHMD
                     SIPLVIISGQVPTHAIGQDAFQEVDTTGITRPCVKHNFLVKDARELAVTIKKAFYIAK
                     SGRPGPVLVDIPKDVQLTKALFHYPDSISMRSYNPVLKGHGGQIKKAIQLVLQAKRPM
                     IYTGGGVILANAAPELTELVRNLGYPCTNTLMGLGGYPATDPLFMGMLGMHGTYEANM
                     AMQHCDVLLAVGARFDDRVIGNPKHFNQEQRQIIHIDIDPSSISKRVKVDIPIVGDVR
                     DVLQEMNRQLAAVKERPDPEALQAWWKQIEQWKARDCLKYDRAASVIKPQLVVEKLYE
                     VTRGELILTSDVGQHQMWAAQFYKFDQPRRWLNSGGLGTMGVGLPYALGAKLAFPDKD
                     VACVTGEASILMCIQELSTAKQYRLPVKVVNLNNRYMGMVRQWQEFFHGNRYSESYMD
                     ALPDFVKLAESFGHVGMRIDKPADVEPALREAFQRKDDLVFMDFITDQTENVFPMVPG
                     GKGLSEMILV"
     gene            complement(5640..6440)
                     /locus_tag="VD791_11890"
     CDS             complement(5640..6440)
                     /locus_tag="VD791_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXV08725.1"
                     /translation="MQSVHDMLERVIAAVKRVAAEEIMPRFLRVAHQRKADGSLFTDA
                     DLAAQEALVRALREIRPVPVVGEEMTQALQAERWLSGDAGLWCVDPIDGTSNFVYGVP
                     YFAVSVALMQQGRSVLGVVYHPIADEAFYAERGGGAYLNGEALPLRAPVARLRSAVAG
                     VDFKRLPVSLAGALAASPPFSSQRNFGAGTLDWCYAAAGRFDLYLHGGQRLWDYAAGS
                     LIFEEAGGSACTLTHDDFWADDLWQRSVIAARDPALFAQWRDWIRARL"
     gene            complement(6599..7150)
                     /locus_tag="VD791_11895"
     CDS             complement(6599..7150)
                     /locus_tag="VD791_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007746699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXV08726.1"
                     /translation="MATAPICDFGRPAIDFDLPATDGKRYTLAGVRGRDGLLVMFICN
                     HCPYVKAVIDRIVRDCTELQSHGIGSVAISANDPTDYPEDSFDNMQRVARQLHFPFPY
                     LWDEKQDVARAYGAVCTPDFFGYNADLQLQYRGRLDVSRTSPVPDAPRELFEAMVQIA
                     RTGKGPKEQVASMGCSIKWRRAA"
     gene            7235..>7705
                     /locus_tag="VD791_11900"
     CDS             7235..>7705
                     /locus_tag="VD791_11900"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HXV08727.1"
                     /translation="MNRPSDPGQVRVPRLFCPGDIAAGALLKLPDQAAHHAVRVLRLG
                     PGDAVRLFNGAGAEWQAEIAGVRKSGVSVRVGARHERDVEAPLRVVLAQAISARERME
                     LTLQKAVELGVAEIQPLETRRSVVRLREERASRRVEHWQNLVAAACEQCGRNRIP"
BASE COUNT         1191 a         2586 c         2595 g         1333 t
ORIGIN      
        1 ggctgatcgc agtcgacttt ctgcaccgct gcgcggcctt cggcgacctg cggcggcggc
       61 tcgcatcccc gatcgcccgc gcgctcgact ggctcgcttg ccaggagcat cagcgcttct
      121 tcctgctgat gcagggcgaa gccagcgact gggccgacat catgccgcgc tcgggcttcg
      181 tgctctacag caacgcgttg tggtatcgcg tcaagcgcct gtaccggctg ccgcgggccg
      241 atgagacgcg gcgctacttc aatcacctgt tccacccgtt ctcggccgga ttggccgagt
      301 atcggcgcgc gcgcctgctc accgattacg tgctgcgcaa ggcgcgcaac cgcgacctgt
      361 atctgagctt cgtcaacttc tcgttcttcg gcgaggaagg cgacgtgttc ggcaacctgc
      421 tcgcgatcct gatgggcctc accagcgcac gggcgtcgcg gcgcgtgctg cgcgccttgc
      481 tcgaagcgcg ggcgcaccgg ccttacccgg tgcgggcggt gtgcgacccg atcacgcgcg
      541 ccgaccctct gtggcgcgat tacatgcagc gccatcgcca gaatctgacc tggcaatacc
      601 acaacggcgg cgtgtggccg ttcgtgggcg gattctgggt gtgcgcgctg gcgcagtcgg
      661 gggaaagcgc gctggcccgc gccgagctgc acaacctggc gcgggcgaat gcgctgggcg
      721 gctgggcgtt cagcgaatgg ctgcatggcc gcaccgctgc accgcgcggc atgcccggcc
      781 agtcctggaa cgccgcgatg tatctgctgg cctgctcggc agtggcgagg atgtgagcgt
      841 ctagcatttt agctcgacca ccatgcccgg atcgaaccgt tcgttcggtt cgtacggcag
      901 gtagccgcgc gggttgcaca ccacgcgcgt tccgcccagg cggtagtcga acgagtaatg
      961 cgtatgcccg tgcacccaca gcagcacggg cggaccgagc aacacctcga ggtcgctcac
     1021 gtaggacgcg ttgatgtgcg agtagccgtt gcgatacggg gccgcgatcg aggcccgcgc
     1081 cggcccgtgg tgcgtgacca ccaccgtccg gccgtcgaac ggctcgcgca gcgccgcctg
     1141 caggaacatg cgcgccgcca ggtggtcggc atgggtgtcc gagggccgca gccgccgcca
     1201 ggccgcggcg cggatatggc ggtagtcgct catgttgtgc tgggcctcgg cctgggccag
     1261 gacgggattg ccggtaaccg cgaaatcagt ccacaaggtc gcggccagga agcgcacgcc
     1321 atccaggacc agcgtctcgc cgtccaagac gtgaacgcag gtgccagcgg ccacacgctt
     1381 catcttggtc agcgtgttct tgaaatgccc gccgtagtac tcgtgattgc ccagcacgta
     1441 gatcaccggc cggtcgaacg tgcgcgccgc ccactcgacg ccgcgggcgc cgacccagat
     1501 gtccccggcg agcagcacca cgtccgcatc ggtggccggc ggctcgaagg gggcgaactc
     1561 gaggtggatg tccgacagga tgtggagctt cacgagcgcg aggcgggcgg tcaaagcccg
     1621 cgattgtatt cattttcgcg ggcgcggccc agggttctgg ggaagctctg aacaagtatc
     1681 gcgctgtgcg accgagcgat tttggcccag ggcaagacgg cgaggacgcc gtgtagttga
     1741 cctacacaag gaggagccgg cgccgccctg ggccaaaatc gccggtccct caaatgcgcc
     1801 atcatatcgg cgggttgcgt caggggcggc gtctgcggcg cgccgctcgt tgccaaggtt
     1861 ccaaccttgg ctgcctcccg gcgcttgcat ccatccgccc ctgacgcaac gcgcagtgcg
     1921 atacttgctc agagcttccc taattcttcg accgatcgac cagccggtcc ttcttcagcc
     1981 acggcatcat ggcgcgcagc ttctcgccga cgatctcgat ctcgtgctcg cggccgatgc
     2041 ggcgcaaggc gttgagcttg gtcgccccgg tctggttctc gagcaggaac tcctgggcgt
     2101 aggcgccggt ctggatctcc ttcaggatgc gccgcatctc gttcttggtg tcctcggtga
     2161 tgatgcgcgg gccgcgggtg aagtcgccgt attcggcgtt gttggagatc gaatagcgca
     2221 tgttggcgat gccgccctcg tacatcaggt ccacgatcag cttcagctcg tgcaggcact
     2281 cgaagtaggc catctcgggc gcgtagccgg cctccaccag gacctcgaac ccggcctgca
     2341 ccagcgcggt ggtgccgccg cacagcacca cctgctcgcc gaacaggtcg gtctcggtct
     2401 cttcccggaa gttggtctcg atgacgcccg cgcgcgcgcc gccgatcgcc gcagcgtagg
     2461 acagcgccag gtcgcgcgcc ttgccgctct tgtcctgcgc caccgcgatc aggcagggcg
     2521 tgccgccgcc ctggacgtag gtggagcgca cggtgtggcc cggcgccttg ggcgcgatca
     2581 tcacgcaatc gatgtcctcg cgcgggatca cctggccgaa gtggatgttg aagccgtgcg
     2641 cgaagccgag cgtcgcgttc ctcttcatgt tcggctccac ctccttgcgg taggtggcgg
     2701 cgatgtgctc gtcgggcatc agcatcatca cgaagtcggc gtccttgacc gcgtcgggga
     2761 cttccttgac cgtgaggccg gcattcaccg ccttggtcca ggacccgccc ttgcgcaagc
     2821 cgacgaccac cttcacgccg gactcgtgca ggttgagcgc gtgggcgtga ccctgcgagc
     2881 cgtagccgat gacggcgatc gtcttgccct tgatcagtga caggtcggcg tccttgtcgt
     2941 agtagacctt catggtgttc tccctagatc ctcaaaaccc ggtcgccgcg cccgatgccg
     3001 gaagcaccgg tgcgcacggt ctccagaatg ctgccgcgct cgagcgcgtt caggaacgca
     3061 tcgagcttgc cggtgtcgcc ggtcagctcg atggtgtagc tcttgtcggt gacgtcgatg
     3121 atgcggccgc ggaagatctc cgccatgcgc ttcatctcct cgcggtcctt gccggccgcg
     3181 cgcaccttca ccagcatcag ctcgcgctcg atgtgctcgc cgtcggacag gtccaccacc
     3241 ttgaccacgt ccaccagctt gttgagctgc ttggtgatct gctcgatgac gtcgtccgag
     3301 ccgctggtga cgatcgtcat ccgggaaagc gtggagtctt cggtgggcgc gacggtcagc
     3361 gactcgatgt tgtaaccgcg tgccgagaac agaccggata cgcgcgacag cgcgcccgac
     3421 tcgttctcca gaagtaacga gatgatgtgc cgcatgctgc atgctcgctg tgactgcgat
     3481 gaatcgatga cgggccatca cgcgcgcgcg gtcccgggaa ggaccggcac gctccgttca
     3541 tcctcatggg atggacggac gctgctcggc gtcgcccgcc gctcttccct acaccaatat
     3601 catctccgac aggcccttgc cgccgggtac catcggaaac acgttctcgg tctggtcggt
     3661 gatgaaatcc atgaacacca ggtcgtcctt gcgctggaac gcttcgcgca gcgccggttc
     3721 cacgtcggcc ggcttgtcga tacgcatgcc gacgtggccg aaggattcgg cgagcttcac
     3781 gaagtcgggc agcgcgtcca tgtaggactc ggaatagcgg ttgccgtgga agaactcctg
     3841 ccactgccgc accatgccca tgtagcggtt gttcaggttc accaccttca ccggcaggcg
     3901 gtactgcttg gcggtggaca gctcctggat gcacatcagg atgctggcct cgccggtgac
     3961 gcaggcgacg tccttgtcgg ggaaggcgag tttggcgccc agcgcgtagg gcaggcccac
     4021 gcccatggtg cccaggccgc cggagttgag ccagcgacgc ggctggtcga acttgtagaa
     4081 ctgcgcggcc cacatctggt gctggcccac gtccgaggtc aggatcagct cgccgcgggt
     4141 gacctcgtag agcttctcca ccaccagctg cggcttgatg accgaggcgg cgcggtcgta
     4201 cttgagacaa tcgcgcgcct tccactgctc gatctgcttc caccaggctt gcagcgcctc
     4261 ggggtcgggc cgctccttga ccgccgccag ctggcggttc atctcctgca gcacgtccct
     4321 gacgtcgccg acgatgggga tgtccacctt cacccgcttg gagatcgacg aggggtcgat
     4381 gtcgatgtgg atgatctggc gctgctcctg gttgaaatgc ttcggattgc cgatcacgcg
     4441 atcgtcgaag cgcgcgccca cggccagcag cacgtcgcag tgctgcatcg ccatgttggc
     4501 ctcgtaggtg ccgtgcatgc ccagcatgcc catgaacagc gggtcggtcg ccgggtagcc
     4561 gcccagcccc atcagcgtgt tggtgcaggg gtagccgagg ttgcgcacca gctcggtcag
     4621 ctcgggcgcg gcgttggcga ggatcacccc gccgccggtg tagatcatcg ggcgcttggc
     4681 ttgcagcacc agctggatcg ccttcttgat ctgcccgccg tgacctttca gcaccgggtt
     4741 gtaggagcgc atgctgatgc tgtccgggta atggaacagt gctttggtga gctgcacgtc
     4801 cttggggatg tccaccagca ccgggcccgg ccggccggac ttggcgatgt agaacgcctt
     4861 cttgatggtg accgccagct cgcgcgcgtc cttcacgagg aagttgtgct tcacgcaggg
     4921 ccgcgtgatg cccgtcgtgt ccacttcctg gaacgcgtcc tggccgatgg catgcgtggg
     4981 gacctggccg ctgatgatca ccagcgggat ggaatccatg tgagccgtgg cgatgccggt
     5041 gaccgcgttg gtcaatcccg gcccggaagt gaccatcacc acgccgacct tgtcggtggc
     5101 gcgcgagtag ccgtcggcgc cgtgcaccgc ggcctgctcg tgccgcacca gaatgtgctt
     5161 gaccttgtcc tgcttgaaca tctcgtcgta gatcggcagg attgccccgc cgggatagcc
     5221 gaacacgaac tggacgccct cctcctgcag acagcggatg gcgatctcgg cgccggtcag
     5281 ctcgacctgc gacgtcactc ccggggcggt catttccatg gttcttgtcc ttggctggtc
     5341 gcggttccct cgtggggtct cgtcccgcag gccgctgtga gggtgggctc ccggaggcgg
     5401 caaggggaac ttatcaagat acctgctcga ccggcaccgg tcaatgaaac cccgcataaa
     5461 gaggcagttg ccgccgtgcc cacggcctta cggcgagaga tgaaccgcgg aggacgccga
     5521 ggacgcggag gaaatgcgct cagacttacc gggtcggcgg gcaggccgca cctggctgta
     5581 cagcagtcga tttcctctgc gccctccgcg tcctccgcgg tcaatacctg cttgacagcc
     5641 tagagacggg cgcggatcca gtcgcgccac tgcgcgaaca gcgccgggtc gcgcgcggca
     5701 atcaccgagc gctgccacag atcgtcggcc cagaagtcgt cgtgagtcag ggtacaggcg
     5761 ctgccgcccg cttcctcgaa gatgagcgag ccggcggcgt agtcccacaa ccgctggccg
     5821 ccgtgcaggt acaggtcgaa gcgtcccgcg gccgcgtagc accagtcgag cgtgccggcg
     5881 ccgaagttgc gctgcgagga gaacggcggc gaggcggcga gcgccccggc caggctcacc
     5941 ggcaggcgct tgaagtccac gcccgcgacc gcgctgcgca gccgggcgac cggtgcacgc
     6001 aacggcagcg cctcgccgtt gagataagcg ccgccgccgc gctcggcgta gaacgcctcg
     6061 tcggcgatcg ggtggtacac cacgccgagc acgctgcgcc cctgctgcat cagggcgacc
     6121 gagacggcga agtacggcac accgtagacg aagttcgacg tgccgtcgat cgggtccacg
     6181 caccacaggc cggcgtcgcc ggaaagccag cgctcggcct gcagcgcctg cgtcatctcc
     6241 tcgcccacca cgggaaccgg tcgaatctca cgcagcgcgc gcaccagcgc ctcctgcgcc
     6301 gcgagatccg catcggtgaa caggctgccg tccgccttgc gctggtgggc cacgcgcagg
     6361 aagcgcggca tgatctcctc ggccgcgacg cgcttgaccg cggcgatcac gcgctcaagc
     6421 atgtcgtgga cggattgcat gatgtatcga gccggctctc cggccgctgc aggtctatcc
     6481 ggcgcggctg gaccaccccg tcacgctgcg cgtgccaccc ctcctcggag aggaggggaa
     6541 ttcactcccc gcctctcacg aggcggggtg gcgccgcagg cgccggggcg gtgtggtgtt
     6601 atgccgcccg cctccacttg atcgagcagc ccatgctggc gacctgctct ttcggtccct
     6661 tgccggtgcg cgcgatctgc accatcgcct cgaacagctc gcgcggggca tcgggcaccg
     6721 gtgaagtgcg cgagacgtcg agccgcccgc gatactgcag ctgcaggtcg gcgttgtagc
     6781 cgaagaaatc cggcgtgcac acggccccgt aggcgcgcgc gacgtcctgc ttttcgtccc
     6841 agaggtatgg gaacgggaag tgcaattgcc gtgccacgcg ctgcatgttg tcgaaggagt
     6901 cctccgggta gtcggtcgga tcgttggcgc tgatcgccac cgagccgatg ccgtgcgatt
     6961 gcagctcggt gcagtcgcgc acgatacggt cgatcaccgc cttgacatac gggcaatggt
     7021 tgcagatgaa catgaccaga aggccatcgc gcccgcgcac gccggccagc gtatagcgct
     7081 tgccgtcggt ggcgggcagg tcgaagtcga tggccggacg gccgaagtcg cagatgggtg
     7141 cggtagccat gtcgggaacg ccggtgtgaa tcgcgctgtc gcagcgaccg cgcggatggc
     7201 agaatcctat catgaaccga ccttccgacc cggcatgaac cgaccctccg accccgggca
     7261 ggtgcgcgtg ccgcgcctgt tctgccccgg cgacatcgcc gccggcgcct tgctcaagct
     7321 gcccgaccag gccgcgcatc acgccgtgcg cgtgctgcgc ctcggccccg gtgacgcggt
     7381 gcgcctgttc aacggcgccg gcgccgaatg gcaggccgag atcgccggcg tgcgcaagag
     7441 cggcgtgtcg gtgcgcgtcg gcgcgcgcca cgaacgcgac gtggaggcgc cgctgcgtgt
     7501 ggtgctggca caggcgatca gcgcgcgcga gcgcatggag ctcacgctgc agaaggcggt
     7561 ggagctggga gtggccgaga tccagccgct ggagacccgc cgcagcgtgg tgcgcctgcg
     7621 cgaggagcgg gcgagccggc gagtggagca ctggcagaac ctggtggccg ccgcgtgcga
     7681 gcaatgcggc cgcaatcgca tccca
//
LOCUS       DASPGE010000368         3482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_682153, whole genome shotgun sequence.
ACCESSION   DASPGE010000368 DASPGE010000000
VERSION     DASPGE010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682153"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VD791_11905"
     CDS             complement(<1..533)
                     /locus_tag="VD791_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXV08728.1"
                     /translation="MSARLPPVPGEWLDRSRVLTFRFEGEIFSGFAGDVIGSALLASG
                     AHILGRSFKYHRPRGVLSMANHDANALVEDRAHVNLRADVTAVVEGMHLVAVNTFGGV
                     QADRARVVDRLSRLLPVGFYYKAFHRPKALAPGWERFFRSMTGLGAVKHDAPRLVTPK
                     GHDFCDVLVIGAGPSGLA"
     gene            complement(542..796)
                     /locus_tag="VD791_11910"
     CDS             complement(542..796)
                     /locus_tag="VD791_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HXV08729.1"
                     /translation="MKLLTCPINGPRPVSEFVYAGEMRPMPDPDACTDEQWTDYVFNR
                     NGSPGVKKEWWYHVASGVWFIAERDTAADRVLRTYLWGEQ"
     gene            complement(798..2039)
                     /locus_tag="VD791_11915"
     CDS             complement(798..2039)
                     /locus_tag="VD791_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXV08730.1"
                     /translation="MPLGLLRFALSRDYPEPRMFRAPQRLKPAYDAVIIGAGGHGLAA
                     AYYLARDHGIADVAVLEKGYLGGGNTGRNTTIIRSNYLTPEGVAFYDEGVRLFRDLAQ
                     DFDLNLFYSTRGHFTLAHSDSALRTMRWRAEVNKHLGVESELVDPDFVRRRCPWIDLS
                     CGGHAPILGALYHAPGAIARHDAVAWGYGRGADRRGVEIHQQTEVTGIEVKGGKVAGV
                     RTTRGNVSTRKVLCAVAGFTPRILDMVGLRSPITVHPLQAMVSEPMRPWLDQIFVSGS
                     LHIYVSQSARGELVMGASLDPYELHATRSTLDFAEGLSAHILDLFPFLSTAKVNRQWA
                     GLADMTPDFAPIMGRTPVEGFYLDAGWGTWGFKATPVCGKTMSHTLAKDEDHPLIQAF
                     SLSRFARYELTGEKGAASVGN"
     gene            complement(2238..2471)
                     /locus_tag="VD791_11920"
     CDS             complement(2238..2471)
                     /locus_tag="VD791_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08731.1"
                     /translation="MTTASFESWAGSIADIGPIYPMVGSEVWLLIIGLVFWIGWHVIQ
                     TRAESRAYRADIERYGKSQTLRELLDKESRAGQ"
     gene            complement(2485..>3482)
                     /locus_tag="VD791_11925"
     CDS             complement(2485..>3482)
                     /locus_tag="VD791_11925"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08732.1"
                     /translation="TAASIVSGAVIERIRSGAFWILAVGIGSVWWIIDAAWGWHPEGW
                     MVRLLGYHDAYASGVIHAIAGGFALGVLLVLGPRIGKFAADGTPRNLAPRNPWLVAIG
                     LFLIYTGFWGFYAACNVPIFDVQSGDGVFYSATNIYLGPTTLSAITVNFLMSLSGGLM
                     VGYVVSKGDAFWAYSCGLAGIIGASAGNDLYHPIHALLVGGATAAIIYKLHYFVERRF
                     KVDDAVGAVAVHGYAGFFGVVIAGFLLWGYPSSPNTEFAAVNPLGQFIGAVIMFFVLG
                     FIPGWVTAKILAAFGLLRIPREVELMGLDTSAFEELRREAGVIAAAEREALATVK"
BASE COUNT          606 a         1202 c         1166 g          508 t
ORIGIN      
        1 gccaacccgc tgggccccgc gccgatcacc agcacatcgc agaagtcgtg gcccttgggc
       61 gtaaccaggc gcggtgcgtc gtgcttcact gcgcccagcc ccgtcatcga tcggaagaag
      121 cgctcccagc ccggcgccag cgccttgggg cggtgaaacg ccttgtaata gaaccccacc
      181 gggagcaggc gcgagaggcg gtcgaccacg cgggcgcggt cggcctgcac cccgccgaag
      241 gtgttcacgg cgaccagatg catgccctcg acaacggccg tgacgtcggc gcgcagattg
      301 acgtgcgcgc ggtcctcgac cagcgcgttg gcgtcgtgat tcgccatcga caacacgccc
      361 cgcgggcggt ggtacttgaa gctgcggccc aggatgtggg cccccgaagc gagcagcgcg
      421 ctgccgatca catcgccggc gaacccgctg aagatctctc cttcgaagcg gaaggtcagc
      481 acgcgcgagc ggtcgagcca ttcgccgggc accggcggca gccgcgcgct catgcctcgg
      541 gtcactgttc accccacaag taggtgcgca gaacgcggtc ggcagcggta tcgcgctcgg
      601 cgatgaacca cacgccgctc gccacgtggt accaccattc cttcttgacg ccgggcgagc
      661 cgttgcggtt gaacacgtag tcggtccact gctcgtcggt gcaggcatcc gggtccggca
      721 tcggccgcat ctcgccggca tagacgaact cggacaccgg ccgcgggccg ttgatcggac
      781 aggtcagcag tttcatttca attgcccacc gacgccgcgc ccttctcccc ggtgagctcg
      841 tagcgcgcga agcgcgacag cgagaacgcc tggatcagcg gatggtcttc gtccttggcg
      901 agggtgtggc tcatggtctt gccgcagacc ggcgtcgcct tgaagcccca agtgccccag
      961 ccggcatcga gatagaagcc ctccaccggc gtgcggccca tgatcggcgc gaagtccggc
     1021 gtcatgtccg ccaggcccgc ccactggcgg ttgaccttgg cggtggacag gaacggaaac
     1081 agatcgagga tgtgcgccga caatccttcc gcgaaatcca gtgtcgagcg cgtggcatgc
     1141 agctcgtagg ggtcgagcga ggcgcccatc accagctcgc cgcgcgccga ctggctgacg
     1201 tagatgtgca ggctgccgga cacgaagatc tgatcgagcc agggccgcat cggctcgctc
     1261 accatggcct gcagcgggtg cacagtgatc ggcgagcgca gccccaccat gtcgaggatg
     1321 cggggcgtga acccggccac ggcgcacagc accttgcggg tcgagacgtt cccgcgcgtg
     1381 gtgcgcacgc cggcgacctt gccgcctttc acctcgatgc cggtgacctc ggtctgctgg
     1441 tggatctcca ccccgcggcg atcggcgccg cgcccgtagc cccaggccac cgcgtcgtgg
     1501 cgtgcgatcg ctccgggcgc gtgatacagc gcgccgagga tcggcgcgtg ccccccgcag
     1561 ctcaggtcga tccacgggca gcgccggcgg acgaagtcgg gatcgaccag ctcgctctcc
     1621 actccgagat gcttgttcac ctcggcgcgc cagcgcatcg tgcgcagcgc cgagtcgctg
     1681 tgggccagcg tgaagtggcc gcgtgtggaa tagaacaggt tcaggtcgaa gtcctgggcg
     1741 agatcgcgaa acagccgcac gccctcgtcg tagaacgcca cgccctcggg ggtgaggtag
     1801 ttggagcgga tgatggtggt gttgcggccg gtgttgccgc cgcccagata gcccttctcc
     1861 agcaccgcca cgtcggcgat gccgtggtcg cgcgccaggt aataggccgc cgccagcccg
     1921 tgaccgccgg cgccgatgat caccgcatcg taggccggct tgaggcgctg cggggcgcgg
     1981 aacatgcgcg gctcgggata gtcgcggctc agcgcgaagc gcagcagacc gagaggcatg
     2041 caagccaccg catcggaagg gctggggtgc gtctattgtc ggcgcgcgct cccggggtgt
     2101 caataacccc gttcaacaaa ctttcccagg atgaaacacg gcgggcgccg ggccgcggcg
     2161 agaatggcgg cgcagcggtt cctccaaccc ctcacccccg cccctctccc gcaaggggag
     2221 aggggaggtt ggtggttcta ctggccggcg cggctttcct tgtcgagcag ctcgcgcagg
     2281 gtctgggact tgccgtagcg ctcgatgtcc gcgcgatacg cgcggctctc ggcgcgggtc
     2341 tggatcacgt gccatccgat ccagaacacc agcccgatga tcagcagcca cacctcgctg
     2401 ccgaccatcg ggtagatcgg gccgatgtcg gcgatgctgc cggcccagct ctcgaagctc
     2461 gcggtggtca tagttctctc cctgtcattt gacggtggcg agcgcctcgc gctcggcggc
     2521 ggcgatgacg ccggcctcgc ggcgcaactc ttcgaaagcc gacgtgtcca ggcccatcag
     2581 ctcgacttcc cggggaatgc gcaacaggcc gaacgcggcc aggatcttgg cagtgaccca
     2641 gccggggatg aacccgagca cgaagaacat gatcaccgcg ccgatgaact gcccgagcgg
     2701 gttgaccgcg gcgaactcgg tgttcggcga ggacggatag ccccacagca ggaagccggc
     2761 gatcaccacg ccgaagaagc cggcatagcc gtgcaccgcc accgcgccca cggcgtcatc
     2821 gaccttgaag cgtcgctcga cgaagtaatg cagcttgtag atgatggcgg ccgtcgcgcc
     2881 ccccaccagc agcgcatgga tcggatggta tagatcgttg cccgcagagg cgccgatgat
     2941 gccggcgagc ccgcaggagt acgcccagaa cgcgtctccc ttggacacga cgtacccgac
     3001 catcagcccg ccggacaacg acatcaggaa gttgaccgtg atcgcggaca gcgtggtcgg
     3061 acccaggtag atattggtgg ccgagtagaa cacgccgtcg ccgctctgca cgtcgaagat
     3121 cgggacgttg caggcggcgt agaagcccca gaagccggtg tagatcagga acagcccgat
     3181 cgccaccagc cagggattgc gcggggcgag gttgcgcggc gtgccgtcgg cggcgaactt
     3241 gccgatgcgc ggccccagca ccagcagcac acccagagcg aagcccccgg cgatggcgtg
     3301 aatcacgccg ctcgcatacg cgtcgtgata cccgagcagc cgcaccatcc agccttccgg
     3361 atgccagccc caggcggcgt cgatgatcca ccacaccgag ccgatcccca ccgccaggat
     3421 ccagaaggcg ccggaacgga tgcgctcgat caccgccccc gacacgatcg acgcagcggt
     3481 cc
//
LOCUS       DASPGE010000369         3237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_97167, whole genome shotgun sequence.
ACCESSION   DASPGE010000369 DASPGE010000000
VERSION     DASPGE010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97167"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(390..968)
                     /locus_tag="VD791_11930"
     CDS             complement(390..968)
                     /locus_tag="VD791_11930"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HXV08733.1"
                     /translation="MIRSLVSLALAALVGAVLVLGGVWLAWRAGAGWFQAETRTESHT
                     IVEQIRMIAKLQTVEYHGTNTVKLEKEDWKGSNAAIYLLEGTVVATLDLQRMTFEVSG
                     NGPERTVRIQLPPVVVEDPMVDRFEIIMSCASFLTAPKLADEDRNALHREALIGLKAA
                     ANKFGIRDRAVRQAQDYLNTFLTALGYKVVFA"
     gene            complement(1049..2044)
                     /locus_tag="VD791_11935"
     CDS             complement(1049..2044)
                     /locus_tag="VD791_11935"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV08734.1"
                     /translation="MSASRPALDNRQPYHSVRSRAAREVLDEHYDLGASLYKFWASPR
                     SVLGAILRGWERCEHPAELHYAWDLERASSLDDGIRETTRRAIALLALDGRAAPRLYE
                     PGCGIGGGVTQVAQELPGAFVAGMTLVQRQAHIGRARARALGLANAHLCCGNYLAAPF
                     ADASFDGIFAIETAVYTPVSERSRLMREMFRLLAPGCRLAVLDGVKLREPANAAERAW
                     IQDVLDGWTMPSPATPEEFAVHARAAGFEVLQQKDVTAHVYASARRIASIATHVLLPL
                     SVLARIPLLGRGMHPLGFASPRHARRFVAACRSQIGVFDAGLGAYYAQVFRKPAR"
     gene            2060..2815
                     /locus_tag="VD791_11940"
     CDS             2060..2815
                     /locus_tag="VD791_11940"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HXV08735.1"
                     /translation="MTWKRMIRPILLRHVAAALCAGVVALSASSAEAPLLFEWRPGAG
                     EIAEGWRALTFRNIARHTRYSLVREGREYVVKAEAEASASGLIRRIDVDARSYPVLRW
                     RWKVENLLTKADVTRKQGDDYPARIYVAFAYDPKRVGVGQRIKYQAARLIYGEYPPHA
                     GLNYIWEGKAAVGTVVPNPFTERVRMIVVESGPANLRRWREYERNVYQDYRAAFGEDP
                     PSISGVAIMTDADNTGESAVAYYGAVGLYPQGQ"
BASE COUNT          523 a         1099 c         1109 g          506 t
ORIGIN      
        1 cacgccctgc gcgagcagcg tgcctgcgct gagcgcagac gccatcgcgg cgagcgccgt
       61 cgatgcgggc cggagaaaac ggctcgacca cttcgggttc atgtccttgc ctcctcgtaa
      121 agacattcaa gcaatgagag aagatgccgg gtatggtggt gactggcctc ctcaaggcgg
      181 aggcgaggga ttgagagact ctatgttttt cgggttgtcg tggacggggc ggagttgcgc
      241 aaccccgtcc atagactagc cgcagtcacg cgcggatggc aagaggccgg ttgtcaacgg
      301 cgacgagtcc atgctgccga gtggatcata ccgggggcat ccggcggtgt cggtgggtcg
      361 gccgtcggat gtcgaatgac aggcaacgct caggcgaaga cgactttgta tccgagcgcg
      421 gtgagaaacg tgttcaggta gtcctgcgcc tggcgcaccg cccggtcgcg gatgccgaac
      481 ttgttggcgg cggccttcaa gccgatcagc gcctcgcggt gcagcgcatt gcggtcctcg
      541 tcggccagct tcggcgcggt caggaacgac gcgcaggaca tgatgatctc gaagcggtcc
      601 accatcgggt cctccaccac caccggcggc agctggatgc gcacggtgcg ttccggcccg
      661 ttgccgctca cctcgaaggt catgcgttgc aggtcgagcg tcgcgaccac ggtgccttcg
      721 agcaggtaga tcgccgcgtt gctgcctttc cagtcctctt tctcgagctt gaccgtgttg
      781 gtgccgtggt actccaccgt ctgcagcttg gcgatcatgc ggatctgctc gacgatggtg
      841 tggctctcgg tgcgcgtctc ggcctggaac cagccggcgc ccgcgcgcca ggccagccac
      901 accccgccca gcaccagcac ggccccgacc agcgcagcga gcgcgagtga gacgagactg
      961 cggatcatgt gcacgcctcc tccggcattg cttcaacgaa cggccagcca agcgagcagt
     1021 atgcccgctg ccggcgtggg gtgcgaaatc agcgcgccgg cttgcggaac acctgggcgt
     1081 agtaggcgcc gagcccggcg tcgaacaccc cgatctgcga gcggcaagcg gcgacgaagc
     1141 ggcgcgcatg gcgcggcgaa gcgaatccga gcggatgcat gccgcgcccg agcaacggga
     1201 tgcgcgccag caccgacagg ggcagcagca cgtgcgtggc gatggatgcg atgcgacgag
     1261 cggaggcgta gacgtgcgcg gtcacgtcct tctgctgcaa cacttcgaag ccggcggcgc
     1321 gcgcgtgcac ggcgaactcc tccggcgtcg ccggtgaagg catggtccag ccgtccagca
     1381 catcctggat ccaggcgcgc tcggccgcgt tcgccggttc gcgcagcttc acgccgtcga
     1441 gcaccgccaa ccggcagccg ggcgcgagca ggcggaacat ctcgcgcatc aggcgggacc
     1501 gttcgctcac cggcgtgtac accgcggtct cgatggcgaa gatgccgtcg aagctggcgt
     1561 ccgcgaacgg cgctgcgaga tagttaccgc agcacaggtg ggcattggcg agccccagcg
     1621 cgcgggcccg ggcacgaccg atgtgcgcct gccgctgcac cagcgtcatg ccggcgacga
     1681 aggcgccagg cagctcctgc gccacctgcg tgacgccgcc gccgatgccg cagcccggct
     1741 cgtacagccg cggcgcggcg cgcccgtcga gcgcgagcaa cgcgatcgcg cgccgcgtgg
     1801 tttcgcggat gccgtcgtcg agcgaagatg cgcgctccag atcccatgca taatgcaatt
     1861 cggcggggtg ctcgcagcgc tcccagccgc gcaggatagc gcccagcacg ctgcgcgggc
     1921 tcgcccagaa cttgtagagg ctggcaccca gatcgtagtg ctcgtccagc acctcgcgcg
     1981 cggcgcgcga ccgcaccgaa tggtacggct ggcggttgtc gagagcgggg cgggaggccg
     2041 acatgacgcg gattcctcga tgacgtggaa gcgaatgata cggccgatcc tcttgcgcca
     2101 cgttgccgcg gccctctgcg ccggtgtcgt tgccctgagc gccagctcgg cggaagcgcc
     2161 gctgctcttc gagtggcggc ccggcgcggg cgaaatcgcg gagggctggc gcgcgctcac
     2221 cttccggaac atcgcccggc atacgcgcta cagcctggtg cgcgaaggcc gggagtacgt
     2281 ggtcaaggcc gaggccgagg cgtcggcctc cggcctgatc cgccgcatcg acgtcgacgc
     2341 acgatcctat ccggtgctgc gctggcgctg gaaggtcgag aacctgctca ccaaggccga
     2401 cgtcacacgc aagcagggcg acgactatcc cgcgcgcatc tacgtcgcct tcgcctacga
     2461 ccccaaacgt gtgggagtcg gccagcgcat caagtaccag gccgcccgcc tgatctacgg
     2521 cgagtacccg ccgcacgccg gactcaacta catctgggag ggtaaggccg cggtgggcac
     2581 cgtggtgccc aatcccttca ccgaacgcgt gcgcatgatc gtggtggaga gcggcccggc
     2641 caacctgcgc cggtggcgcg aatacgagcg caatgtctat caggactatc gcgccgcttt
     2701 cggcgaagac ccgccgtcga tctccggcgt cgccatcatg accgacgccg ataacaccgg
     2761 tgagtccgcg gtcgcctatt acggcgccgt aggtctctac ccgcagggac agtagcgccc
     2821 ggcgcgcgct cggtgtccta gcggggccta ccactttcgt tctatcgcct tcccctgtcg
     2881 cagcacctac tctgtgacgg cggttgcatt tgggaccgca tgcctgtgcc atggcgccgg
     2941 ctgcccctgc cggccagcgc cttgggcgcc ggtccgcaat tcctctatcg ggaggcgcta
     3001 tgcttcgctt attcgatcct ctgcaagtgc ctgtgactgg cgctacccgt ccggaagccc
     3061 accggcaggc gacgcgcagg tcaccgtcgc agagcggaga agccatggca ggcgcgttcg
     3121 tgatcgtgac cgctccggga cgggagccaa gctcgctggc ggaatgcctg cggcgaatcg
     3181 acggcgcgcg cccgatcctg tttgccgggc ccgcggccat cgagctcgaa ggatctc
//
LOCUS       DASPGE010000370         9451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1501749, whole genome shotgun sequence.
ACCESSION   DASPGE010000370 DASPGE010000000
VERSION     DASPGE010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9451
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501749"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VD791_11945"
     CDS             complement(<1..467)
                     /locus_tag="VD791_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HXV08736.1"
                     /translation="MSTQLVTNARIVTADEVFCGTLEVRDGVFARLDTGLTVLPRAQD
                     WGGDYLIAGQIELHTDNLEKHLEPRPGVRWPALSALLTHDAQISSAGITTVLDAMGVG
                     DFDARSVRAQGLEDAAQALRHARAHGLLRAEHLLHMRCELACDNMLEVVAPFL"
     gene            509..1213
                     /locus_tag="VD791_11950"
     CDS             509..1213
                     /locus_tag="VD791_11950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1045 domain-containing protein"
                     /protein_id="HXV08737.1"
                     /translation="MHPRYAVYFAPRDGSSLWQLGCSWLGRDPQRDCALEQPSLPGFT
                     HARIADVTAAPRAYGFHATLKAPFALVDGCAAADLHAALAAFARQRTPFTLPRLEVGM
                     LGGFIALRPVSASSALDALARDCVVELDHWRAPPHADELARRRAASLSARQEAMLARY
                     GYPYVLDEFRFHLTLTDRMPAHEAQSLRELLAEHFTEALRQRLAVDSICLFVQERPGV
                     PFTLARRYDFLAVSRS"
     gene            1322..2065
                     /locus_tag="VD791_11955"
     CDS             1322..2065
                     /locus_tag="VD791_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08738.1"
                     /translation="MPVLIALTAVIQAFFVFHVYRTGRPYWWAFIILSFPVIGCVAYY
                     ALEVFPGSREHRSARRAASQVARAFNPTGGLKARLEALAVCPSVANKIAAADEFMRCG
                     VFGEAVRLYRDALTGPHADDPNLLLGLARAHVNDGTLVEAQEVLARLRQLDARYRPEE
                     ARLLMARTHEGLGETEQALAEYEEVAQVYIGLEAKCRYGMLLKQLGFAVQANSVFKEM
                     LEHAKRFKVNLDTERPWIDTARRNLMEAA"
     gene            complement(2083..3369)
                     /locus_tag="VD791_11960"
     CDS             complement(2083..3369)
                     /locus_tag="VD791_11960"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV08739.1"
                     /translation="MADMQRLLAKAEAGDASAQYAVGNAYHLGLGVPKSLPLAMRWYL
                     KAAAQGHVDAQVNLGIVFIHDLGAAGGTRNPVQARYWFKRAAELGDAQAMAYLARIFL
                     EGDGVPPNPARACDWLQRAGEQGHVDAFNELGVLLDSGRVGTPDHAGAAAAYERGARL
                     GDMRAQYNLANCYLAGRGVEPNPALAAQWYRAAADQGLGDAQYALATLLARGVEGVER
                     DPGQALKWLAKAADKGVAQAQYELAKQLRAGDGVAADPLQAMHYYHEAADQGHADAMF
                     SLALMLEVGAGLDRAYPERAAGWYRRLAQQHGHGGAAHNLGILLAQGKGVPKDAGAAR
                     ELFELAISLGADEAMHSLALLLVRGEGIAQDIVEGAKWAILALRDDPNDDVRKLFDVI
                     SALLPPQLMEQAQARAASWQRTAKTVQWTSEVADTA"
     gene            complement(3459..3986)
                     /locus_tag="VD791_11965"
     CDS             complement(3459..3986)
                     /locus_tag="VD791_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08740.1"
                     /translation="MLRTLAAAWLLTLIAGPCAAQTTWERNADWIEEQIEDCAEGWDT
                     TACRYFSARALSRLFGVPEVCAGESCMNPTQLAERIAKGDPWAELGAATEQAVLDQAQ
                     QMATGGLPVVAVNESVGWVVLVMPGPLYPSVRWRRNVPVAVGTRLDEPYASVYGQGLN
                     FLFSDPSKVTLYVHK"
     gene            complement(4013..5539)
                     /locus_tag="VD791_11970"
     CDS             complement(4013..5539)
                     /locus_tag="VD791_11970"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HXV08741.1"
                     /translation="MNKPLERSALQIKDIPHWIDGRETPGASGRHGEVYDPALGRVAG
                     RVGYASAEEVGRAVACAQAAFPGWAATPPLRRARVLFKFKELIERNLDALARLISSEH
                     GKVVSDARGEVTRGLEVVEFACGIPHLLKGEFSEQVGTGVDSWSVRQPLGVCAGITPF
                     NFPAMVPMWMFPMALACGNSFVLKPSERDPSASVLLARLLQEAGLPDGVLNVVHGDKV
                     AVDALLQDPRIAAVSFVGSTPIAEYIYRTGTAAGKRVQALGGAKNHLVVMPDADLEQA
                     VDALVGAAYGSAGERCMAISVAVAVGGVADALVERLAQRARSLRVNAGVDENAEMGPL
                     VTRQHLDKVLGYVAKGVEEGARLVVDGRGLAVPGYERGFFLGGCLFDQVRPDMSIYRE
                     EIFGPVLCVLRAPDFDTAVDLINRHEYGNGVSIFTRDGDAAREFATRIQIGMVGINVP
                     IPVPMAYHSFGGWKRSLFGDHYVHGPEGVRFYTHLKTITSRWPTGIRKGAEFVMPTMR
                     "
     gene            complement(5554..7047)
                     /locus_tag="VD791_11975"
     CDS             complement(5554..7047)
                     /locus_tag="VD791_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HXV08742.1"
                     /translation="MKFVIAQMQHETNTFSPVPTPWEAFGNNGPFLGRAAYEAMKGTR
                     LPMAAYIDLAEDAGAQLATPVAAWANPSGPVDGAAYDRICALICDAVAQGCDAVFLDL
                     HGAMVVADRTDDGEGTLLERIRAIAPRVPIAVSLDLHANLTERMVRNCEVIAGYKTYP
                     HVDQYEAGHLAGSILLRALEGKVKPAMAWGNRPLLAQTLRENTGERPMKDFVDAARAA
                     ERHGMLAATAFGGFQQADIHDAGISVVTVADGDRAKAQAACEAILDVAWRQKEEFVYR
                     GEPLQQAIARAKRMADESGGPILLLDHADNCASGATQDTMCVLEEALRQGLTGIAVGP
                     VRDPEAVDRMVRAGVGARLALKVGGKMDMPAIGEKGRPLELTGVVRAITDGEYTITGP
                     QFQGMRCYMGRTAVLDTGAAEVVVIERNQEPWDRGVFTSVGIDPAAKRFLLLKSRMYF
                     RPVFLPIAKGMVFCDSIGVGSSDWTRFEYRKLRRPIYPLDEFPAAHR"
     gene            complement(7159..8493)
                     /locus_tag="VD791_11980"
     CDS             complement(7159..8493)
                     /locus_tag="VD791_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXV08743.1"
                     /translation="MGTREQELLAQLNLEPLWMPFTANRQFKAQPRLLVAAKDMHYTS
                     HDGRQILDGIAGLWAVNAGHCRAPIVEAIKKQAESVDYVTGFQLHHPSAFLAAERLTR
                     IAPAGMDHVFFVNSGSEAVDTALKMALAYHRVRGQGTRTRFIGRERGYHGVGFGGVSV
                     GGMTANRKAFSPALLPGVDHLPHTHLPEKNAFSRGQPEHGAHLADDLERLVALHDASN
                     IAAVIVEPVAGSTGVLVPPKGYLQRLRQICDKHGILLIFDEVITGFGRTGSAFAAHTF
                     GVTPDLITCAKALTNAAVPMGAVIVKKELYEAFMSGPEGIEFYHGYTYSGHPLAAAAA
                     IATLDLFKAEGLFQRAAELAPYWEQCVHALRGLPHVIDIRNIGLVAGIELEPIPGKPT
                     ARAFEAFARAYQKGVLIRTTGDTIALSPPLIIEKGQIDQILDTVREILTTLP"
     gene            8699..>9451
                     /locus_tag="VD791_11985"
     CDS             8699..>9451
                     /locus_tag="VD791_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXV08744.1"
                     /translation="MDLNYTADELAFRDTVRSFVRTCLPQDIARKVLEHKRLGKDDHL
                     RWQKILSEQGWMAPQWPKQYGGCEWNAVQQHIFEEECADAGAPRIIPFGVNMVGPVII
                     AFGSDRQRRHYLPRILSNEDWWCQGYSEPGAGSDLASLKTRAERRGDRYLVNGQKTWT
                     TLAQHADMIFCLVRTASTGKKQEGISFLLIDMRSRGVTVRPIITIEGEHEVNEVWFDD
                     VEVPAENLVGEEGRGWTYAKFLLSHERSGIAAV"
BASE COUNT         1419 a         3334 c         3194 g         1504 t
ORIGIN      
        1 tcgaggaaag gcgcgaccac ctcgagcatg ttgtcgcagg ccagctcgca gcgcatgtgc
       61 agcaggtgct cggcgcgcag cagcccgtgt gcgcgcgcat gccgcagggc ttgcgccgcg
      121 tcctccagcc cctgcgcgcg gaccgagcgc gcgtcgaaat cgcccacgcc catggcgtcc
      181 agcaccgtgg tgatgccggc cgaggagatc tgcgcgtcgt gcgtgagcaa cgcggacagc
      241 gcgggccagc gcacgccggg gcgtggctcg aggtgcttct cgagattgtc ggtgtgcagc
      301 tcgatttgtc cggcgatcaa gtaatcgccg ccccagtcct gcgcccgcgg caggacggtc
      361 aggccggtgt ccaagcgcgc gaacacgccg tcgcgcactt ccagcgtgcc gcagaagact
      421 tcgtcggcgg tgacgatgcg ggcgttggtg acgagctgcg tggacatgga cgaagaggta
      481 aagtgtaacg atatccggcc cacgcgtcat gcatccgcgc tacgcagtct atttcgctcc
      541 gcgcgacggc tcatcgctgt ggcagctcgg ctgcagctgg ctgggccgcg atccgcagcg
      601 cgattgcgcg ctggagcagc cgtcgctccc cggtttcacg cacgcgcgca tcgcggacgt
      661 tactgccgcg ccgcgagcgt acggctttca cgcgacgctc aaagccccgt tcgcgctcgt
      721 cgacggctgc gccgccgccg acttgcatgc cgcgctcgcc gccttcgcgc ggcagcgcac
      781 gccgtttaca ctgccgcgcc tcgaggtcgg gatgctgggg ggattcatcg cgctgcggcc
      841 ggtctccgca agcagtgcac tcgacgcgtt ggcgcgcgac tgtgtggtgg agctcgacca
      901 ctggcgtgcg ccgccacacg ccgacgagct cgcgcggcgc cgtgcggcga gcctgagcgc
      961 gcggcaagag gcgatgttgg cgcgctacgg ttatccatat gtcctggatg aattccggtt
     1021 tcaccttacg ttgacagatc ggatgccggc gcacgaggcg caatcgttgc gcgagctgct
     1081 cgccgagcat ttcaccgagg cgctgcgcca gcgtctggcc gtcgacagca tctgcctgtt
     1141 cgtccaggag cgccccggcg tgcccttcac gctcgcgcgg cgctacgatt ttttagcagt
     1201 ttcccggtcg taagtccgcg tttcgacggc ctttccgggg ccgccggagg atgggcatac
     1261 cccttgcgtt tttctatgcc aacacagtgt caactcgggg cagcgttcgg ggagtttcgt
     1321 catgccggtg ctgatcgcgc ttaccgccgt catccaggcg ttcttcgttt tccacgtcta
     1381 tcggaccggg cgtccctact ggtgggcgtt catcatcctg tcgttcccgg tgatcggctg
     1441 cgtggcctac tacgcgctcg aggtgtttcc cggctcgcgc gagcatcgct cggctcggcg
     1501 cgctgcgagc caggttgcgc gtgcattcaa ccccaccggc gggctcaagg ctcggctgga
     1561 agcgctggcg gtgtgtccca gcgtggccaa caagatcgcg gcggccgacg aattcatgcg
     1621 ctgcggcgtg ttcggcgagg ccgtgcgcct gtatcgggac gcgctcaccg gcccccacgc
     1681 cgacgacccc aacctgctgt tgggcctggc ccgtgcgcac gtcaacgacg gcaccctggt
     1741 cgaggcgcaa gaagtgctcg cgcgcctgcg ccagctggac gcacgctacc gacccgagga
     1801 agcgcgcctg ctcatggcgc gcacgcacga agggctgggc gaaaccgaac aggcgctggc
     1861 cgagtacgaa gaggtcgcgc aggtgtacat cgggctggaa gccaagtgcc gctacggcat
     1921 gctgctgaaa cagctcggct tcgcggtgca ggccaactcg gtgttcaagg agatgctgga
     1981 acacgccaag cgcttcaagg tcaacctcga caccgagcgc ccctggatcg acaccgcgcg
     2041 ccgcaatctg atggaagccg cgtaggccgt cacgcttccc gatcacgcgg tgtccgcaac
     2101 ctccgaggtc cactgcaccg tcttggcggt gcgctgccag cttgccgcgc gggcttgcgc
     2161 ctgctccatg agctgcggcg gcagcagcgc ggagatgacg tcgaacaact tgcgcacgtc
     2221 gtcgttcggg tcgtcgcgca gcgcgaggat cgcccacttt gcgccctcga cgatatcctg
     2281 cgcgatgccc tcgccgcgca ccagcagcag ggccaggctg tgcatcgcct cgtcggcgcc
     2341 cagcgagatg gccagctcga acagctcgcg cgccgcgccg gcatccttcg gcacgccctt
     2401 gccctgggcg aggaggatgc ccaggttgtg cgcggcgccg ccatgaccgt gctgctgcgc
     2461 gaggcgccgg taccagcctg ccgcccgctc cgggtaggcg cggtccagcc cagcgccgac
     2521 ctccagcatg agcgcaaggc tgaacatcgc gtcggcgtgg ccctggtccg cggcttcgtg
     2581 atagtagtgc atggcctgca gcggatcggc cgcgaccccg tcgccggcgc gcagctgctt
     2641 ggccagctcg tattgcgcct gtgcgacccc cttgtccgcc gccttcgcca gccacttgag
     2701 cgcctgtccc ggatcgcgct cgacgccctc gacaccgcgc gccaatagcg tggccagggc
     2761 gtactgcgca tcgcccaggc cctggtcggc ggccgcgcgg taccactgcg cggcgagcgc
     2821 cgggttcggc tccacgccgc gcccggccag atagcagttg gccaggttgt attgcgcgcg
     2881 catgtcgccc aggcgcgcgc cacgctcgta agcggccgcc gcgccggcgt ggtcgggcgt
     2941 acccacgcgc ccgctgtcca gcagcacgcc cagctcgttg aaggcgtcca catgcccctg
     3001 ctccccggcg cgttgcagcc aatcgcatgc ccgcgcgggg ttgggcggca cgccatcgcc
     3061 ttccaggaag atgcgggcga gataggccat ggcctgcgcg tcgcccagct cggccgcacg
     3121 cttgaaccag tagcgcgcct ggaccgggtt gcgggtgccg ccggcggcgc cgaggtcgtg
     3181 gatgaagacg atgccgagat tgacctgcgc gtcgacgtgg ccctgcgccg cggccttcag
     3241 gtaccagcgc atcgcgagcg gcaggctctt cggcactccc aaaccgaggt gataggcatt
     3301 gccgaccgcg tactgggccg acgcgtcccc ggcttcggcc ttggccagca ggcgctgcat
     3361 gtcagccatg gatgtcctcg ggcgcctcgg cgagtggtgg tgctgcgcca gcggcagcga
     3421 gaagcatgcc taggaccggc cgcggggagg gtcctgactc acttatggac gtacagcgtg
     3481 accttcgaag ggtcggaaaa caggaaattc agaccctggc cgtacaccga ggcgtacggc
     3541 tcgtccaggc gcgtgccgac cgcgaccggg acgttgcgcc gccagcgcac cgacgggtag
     3601 agaggccctg gcatcacgag cacgacccag ccgacggatt cgttcacggc caccaccggc
     3661 aggcctccgg tggccatctg ctgggcctgg tcgagcacgg cctgctcggt ggccgctccc
     3721 agctccgccc acgggtcccc cttggcgatc cgctcggcga gctgcgtcgg gttcatgcag
     3781 ctctcgccgg cgcagacttc gggcaccccg aacaggcggc tcagcgcacg ggcgctgaag
     3841 taacggcagg cggtggtgtc ccagccctcg gcgcagtcct cgatctgctc ttcgatccag
     3901 tcggcgttgc gctcccacgt ggtctgggcg gcgcaggggc ccgctatcag ggtcaggagc
     3961 caggcggcgg caagcgtgcg cagcatgcat attccctccg ccgccggctt gcctaacgca
     4021 tggtaggcat gacgaactcc gcgcccttgc gtatgcccgt gggccagcgg ctggtgatgg
     4081 tcttgagatg ggtgtagaag cgcaccccct cgggaccgtg cacgtagtgg tcgccgaaca
     4141 gggaacgctt ccagcccccg aagctgtggt atgccatcgg cacgggaatc ggaacattta
     4201 tacccaccat gccgatctgg atgcgggtgg cgaactcgcg ggcggcgtca ccgtcccggg
     4261 tgaagatcga caccccgttg ccgtactcat gacggttgat caggtccacg gcggtgtcga
     4321 agtccggcgc gcgcagcacg cacagcaccg ggccgaagat ctcctcccgg tagatgctca
     4381 tgtcgggacg cacctggtcg aacaggcagc cgcccaggaa gaacccccgc tcgtagcccg
     4441 gcaccgccag gccgcgcccg tccaccacca gccgcgcgcc ttcctcgacg cccttcgcca
     4501 catagccgag caccttgtcc aggtgctgcc gggtgaccag cggacccatc tcggcgttct
     4561 cgtcgacgcc ggcgttgacc ctgagactgc gcgcgcgttg cgccaggcgc tccaccagcg
     4621 cgtcggcgac gcctcccacc gcgaccgcca ccgagatcgc catgcagcgc tcgccggccg
     4681 agccgtaggc cgcaccgacg agcgcatcga cggcctgctc cagatcggcg tccggcatca
     4741 cgaccaagtg gttcttcgcc ccgcccagcg cttgcacgcg cttgccggcc gcggtgcccg
     4801 tgcgatagat gtactcggcg atcggagtgg agccgacgaa gctcaccgcc gcgatgcgcg
     4861 ggtcctgcag cagcgcatcc accgccacct tgtcgccgtg caccacgttg agcacgccgt
     4921 cgggcagccc cgcctcctgc agcagcctgg cgagcaggac gctggccgag ggatcgcgct
     4981 cggagggctt gagcacgaag ctgttgccgc aggcgagcgc catcgggaac atccacatgg
     5041 gcaccatcgc ggggaagttg aacggcgtga tgcccgcgca cacgcccagc ggctggcgca
     5101 ccgaccagct gtccacgccg gtgccgacct gctcggagaa ctcgcccttc agcagatgcg
     5161 ggatgccgca ggcgaactcc accacttcca ggccgcgggt cacctcgccg cgcgcgtcgg
     5221 acacgacctt gccgtgctcg ctcgagatca accgcgccag cgcatcgagg ttgcgctcga
     5281 tcagctcctt gaacttgaac agtacgcgcg cacgccgcag cggcggagtg gccgcccagc
     5341 ccgggaaggc ggcttgcgcg caggccacgg cccggcccac ttcctccgcg ctcgcatagc
     5401 ccacgcggcc ggcgacgcgg cccagcgccg ggtcgtacac ctcgccgtga cggccgctgg
     5461 cgcccggcgt ctcgcgcccg tcgatccagt gcgggatgtc cttgatctgc agtgcagacc
     5521 gttccagcgg tttgttcatc tcgatcctct cgtctatcgg tgcgcggcgg gaaactcgtc
     5581 cagcgggtag atcggccggc gcagcttccg gtactcgaac ctggtccagt ccgaagagcc
     5641 cacgccgatg ctgtcgcaga acaccatgcc cttggcgatc ggcaggaaca ccggccggaa
     5701 gtacatgcgc gacttgagca gcaggaagcg cttcgccgcc gggtcgatac caacgctggt
     5761 gaatactccg cggtcccacg gttcctggtt gcgctcgatc accaccacct cggcggcgcc
     5821 ggtgtccagc accgcggtgc ggcccatgta gcagcgcatg ccctggaact gcggaccggt
     5881 gatggtgtac tcgccgtcgg tgatcgcgcg caccacaccg gtcagctcca gcggccgccc
     5941 cttctcgccg atggccggca tgtccatctt gccgcccacc ttcaacgcaa gccgcgcgcc
     6001 gacgccggcg cgcaccatcc ggtccaccgc ctccggatcg cgcaccggtc ccacggcgat
     6061 accggtcaac ccctggcgca acgcttcctc gagcacgcac atcgtgtcct gcgtggcgcc
     6121 cgaggcgcag ttgtcggcgt ggtcgagcag caggatcggc ccgccgcttt cgtccgccat
     6181 gcgcttggcg cgcgcgatcg cctgctgcag cggctcgccg cggtagacga attcctcttt
     6241 ctgccgccag gcgacgtcca ggatcgcctc gcacgccgcc tgcgccttcg cccggtcgcc
     6301 gtcggccacg gtcaccacgc tgatgcccgc gtcatggatg tccgcctgct ggaagccgcc
     6361 gaaggcggtg gccgccagca tgccgtgccg ctcggccgcg cgcgcggcgt ccacgaagtc
     6421 cttcatcggc cgctcgccgg tgttctcgcg cagcgtctgc gccagcagcg ggcggttgcc
     6481 ccaggccatc gccggcttga ccttgccttc cagcgcgcgc agcaggatgc tgccggccag
     6541 atgcccggcc tcgtactgat cgacgtgcgg ataggtcttg tagccggcga tcacctcgca
     6601 gttgcgcacc atgcgctcgg tgaggttggc gtgcaggtcc agggataccg cgatcggcac
     6661 gcgcggggcg atggcgcgga tgcgttccag cagcgtgcct tcgccgtcgt cggtgcgatc
     6721 ggccaccacc atggcgccgt gcagatccag gaacaccgcg tcgcagccct gcgccaccgc
     6781 gtcgcagatc agcgcgcaga tgcggtcgta ggcggcgccg tccaccgggc cgctcggatt
     6841 ggcccaggcg gcgaccggcg tcgcgagctg cgcgccggcg tcctcggcga gatcgatgta
     6901 ggccgccatc ggcagccgcg tgcccttcat cgcctcgtag gccgcgcggc ccaggaacgg
     6961 gccgttattg ccgaacgcct cccagggcgt gggcacgggc gagaacgtgt tcgtctcgtg
     7021 ctgcatctgg gcgatgacga acttcatcga cagaccttcg ctcgatcaac aagaaacccg
     7081 ccagggcgcc aaggcgccgc gaagaaaagc cagagaaatc cttggcgttg cctctgcgtc
     7141 tttgcggtta acgggtcctc agggcagcgt ggtgaggatc tcgcgcacgg tgtccaggat
     7201 ctggtcgatc tggcccttct cgatgatcag cgggggcgac agcgcgatgg tgtcgccggt
     7261 ggtgcggatc agcacgcctt tctggtaggc gcgcgcgaac gcctcgaagg cgcgcgcggt
     7321 gggcttgccg gggatcggct ccagctcgat gccggccacc aggccgatgt tgcggatgtc
     7381 gatgacatgc ggcagcccgc gcagcgcgtg cacgcactgc tcccagtacg gcgccagctc
     7441 ggcggcgcgc tggaacaggc cttcggcctt gaacagatcc aaggtcgcga tcgcagcggc
     7501 cgcggccagc gggtgccccg agtaggtgta accgtggtag aactcgatgc cttccggtcc
     7561 gctcatgaag gcctcgtaga gctccttctt cacgatcacc gcgcccatcg gcacggcggc
     7621 gttggtcagc gccttggcgc aggtgatcag gtccggcgtc acgccgaaag tgtgcgccgc
     7681 gaaggcgctg ccggtgcggc cgaagccggt gatgacttcg tcgaagatga gcagaatgcc
     7741 gtgcttgtcg cagatctgcc gcaggcgctg caggtatcct ttcggcggca ccaggacgcc
     7801 cgtggagccg gccacgggct ccacgatcac ggcggcgatg ttggaggcgt cgtgcagcgc
     7861 caccagccgt tcgaggtcgt cggccaggtg cgcgccgtgc tcgggctgcc cgcgcgagaa
     7921 cgcattcttc tccggcaagt gcgtgtgcgg aaggtgatcc acgccgggca acagcgccgg
     7981 gctgaaggcc ttgcggttgg cggtcatgcc gcccaccgac accccgccga agccgactcc
     8041 gtgatagccg cgctcgcgtc cgatgaagcg cgtgcgcgtg ccctggccgc gcacgcgatg
     8101 gtaggcgagc gccatcttga gcgcggtgtc caccgcctcc gagccggagt tgacgaagaa
     8161 cacgtgatcc attcccgcgg gtgcgatgcg ggtcagccgc tcggcggcca ggaaggccga
     8221 cgggtggtgc agctggaagc cggtcacata gtccacgctc tccgcctgct tcttgatcgc
     8281 ctcgacgatc ggcgcgcggc agtggccggc gttcaccgcc cacagcccgg cgatgccgtc
     8341 caggatctga cgcccgtcgt gagaggtgta atgcatgtcc ttggccgcca ccagcaagcg
     8401 cggctgcgcc ttgaactggc ggttggccgt gaacggcatc cacagcggct cgagattgag
     8461 ttgagcgagc agctcctgtt cgcgggtacc catgattcct cctggaagcg gtgcggcgat
     8521 tggcgccgat gcgtgcggcg gcgataagag gttgaattat agacccgaag gttttgaatt
     8581 atagaccggg cgcaccgact cccgccaccg ccgcgcgcgc ggttgcccgg ggctgcgcca
     8641 tcccgtatgc tgcgaagcgc tgcacgcgct ctcgaccatc cccccgcgga acctccccat
     8701 ggacctgaac tacaccgccg acgagctggc tttccgcgac acggtgcgca gcttcgtgcg
     8761 cacctgcctg ccgcaggata tcgcgcgcaa ggtgctggag cacaagcggc tcggcaagga
     8821 cgatcacctg cgctggcaga agatcctctc cgagcagggc tggatggcgc cgcagtggcc
     8881 gaagcagtac ggcggctgcg agtggaacgc cgtgcagcag cacatcttcg aggaggagtg
     8941 cgccgacgcc ggcgcgccgc gcatcatccc gttcggcgtg aacatggtcg ggccggtgat
     9001 catcgctttc ggcagcgacc ggcagaggcg ccactacctg ccgcgcatcc tgtcgaacga
     9061 ggactggtgg tgccagggct actccgagcc cggcgccggc tcggacctgg cttcgctcaa
     9121 gacccgcgcc gagcgccgcg gcgaccgcta cctggtcaac gggcagaaga cctggaccac
     9181 tctggcgcag cacgcggaca tgatcttctg cctggtgcgc accgccagca ccggcaagaa
     9241 gcaggagggc atctccttcc tgctgatcga tatgcgctcg cgcggggtga cggtgcggcc
     9301 gatcatcacc atcgaaggcg agcacgaggt caacgaagtc tggttcgatg acgtcgaagt
     9361 cccggccgag aacctggtgg gcgaggaggg ccgcggctgg acctacgcca aattcctgct
     9421 gtcgcacgag cgctccggga tcgccgccgt g
//
LOCUS       DASPGE010000371         4564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_565626, whole genome shotgun sequence.
ACCESSION   DASPGE010000371 DASPGE010000000
VERSION     DASPGE010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565626"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..596
                     /locus_tag="VD791_11990"
     CDS             108..596
                     /locus_tag="VD791_11990"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXV08745.1"
                     /translation="MNLRREAASRRTRVTGDTRAALDLGMLPGLLGYRLRLAQRAVFE
                     DFAASVGHAEVSPGLFGILIIVEANPGLKQSQLAAAAHLDRSSLVPVLDKLEARGHVT
                     RRPSPDDRRVNGLWLTPAGAALLRTLKRKVLAHEQRVMRGMSETQRAQLFDLLARVTP
                     ET"
     gene            complement(609..1037)
                     /gene="arfB"
                     /locus_tag="VD791_11995"
     CDS             complement(609..1037)
                     /gene="arfB"
                     /locus_tag="VD791_11995"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HXV08746.1"
                     /translation="MASAVRVSARVSLQESELQETFVRAPGPGGQNVNKVASAVQLRF
                     DVAGSPSLPEDVRERLRRLAGRRLTAAGVLVIEAHRYRTQVRNRDDARERLFGLIRQA
                     SVPPKPRKATKPSAAARERRLREKRLRAQVKRTRGARDVE"
     gene            1323..3083
                     /locus_tag="VD791_12000"
     CDS             1323..3083
                     /locus_tag="VD791_12000"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV08747.1"
                     /translation="MNRWKTQLAGVAKAMSAAALVGFAAQAGAAEITWDRLLNADRDP
                     NNWLMYHGSFKGWHYSGLDQINKNNVKNLGIAWIHTPSASKRGIQSFPLVADGILYYT
                     SSTGQVWALDAANGAFVWKYTAKIDQERAEGTFYNPYSRGLAVGYGKVYVGTVDGRMI
                     ALDQKTGQVVWDNMILTVEKGNKGFTGAPVIVKDKVLIGSNGGELSGCCGPIFAVNAQ
                     TGEVEWQFDTIGGDERSRQSWGNDSWKVGGGGGWMTGSYDAKTNSVWWGTANPAPDYD
                     WAGENWMTEGPRPGTNLYSSSVVVLDADTGKLKAYFAEMPHDAWDFDSAVGEFVQIDR
                     GGKRYMVHPNKGGVMFVYNADPDQTQGDQVLKVENAYMIGKTYNYIKGVNPKTGELIG
                     RRELPLGKHTNVCPAIDGAISWNTGAYNPNNGLYYKVTQEWCFDIEVQKVDRPADFSG
                     QAYFGASWTARHPEGRQAFGTVQAFDPVTSKKRWEIELKYPPLASLLTTKGGLVFVPG
                     ADGTFYALDADNGSKLWQHNNGIGHHGGVISYTAKGKQYVAVVTGWGSHVSGNYGPLF
                     GEPFASMPTDAGQLLVFSVK"
     gene            3192..3500
                     /locus_tag="VD791_12005"
     CDS             3192..3500
                     /locus_tag="VD791_12005"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXV08748.1"
                     /translation="MKPLILMALAVACGAPMAASADDKDSDAIKKLFAKNCALCHFNY
                     GLDPGRGAPKLAGTQLSLKGVYDRIANGKQGVMPGFKKLLTEQEIQALADYIKALPAN
                     "
     gene            3642..4016
                     /locus_tag="VD791_12010"
     CDS             3642..4016
                     /locus_tag="VD791_12010"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXV08749.1"
                     /translation="MSNILRSTLPAYAMVAALSSTATALAQTQDIDTAIAPAVQQVNE
                     KYSEHNLDIKKLFAQKCSWCHQGYGMRQADGPKLAGTDKTKEQLMKQIANGKSPMPGF
                     KSQLKEHELQALAEYIKALPKN"
     gene            complement(4069..4461)
                     /locus_tag="VD791_12015"
     CDS             complement(4069..4461)
                     /locus_tag="VD791_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08750.1"
                     /translation="MTRRNLPRMLGVALMLAMAAVAWRTHAADTAAVDGTWEGPWYRG
                     MSSGRATFHIRDGGGSMQLTNAESFGEEPKPLSAVAFDGSTFKFRADGGGGPMTAALK
                     LNDRGDQMKGMGKYEGFGVRFELSRVRP"
BASE COUNT          817 a         1566 c         1483 g          698 t
ORIGIN      
        1 actccaattg ttggagatca aacaaattgc cccgtgagcg ccgtccgctc gcccgcgcgc
       61 ggccccacgc cgggccgcgc gcgccgcgcc gcccgggcgc cccggcggtg aatctcagac
      121 gcgaggcggc gtccaggcgc acgcgcgtca ccggcgacac gcgcgccgcg ctcgatctcg
      181 gcatgctgcc gggtctgctg ggctatcgcc tgcggctcgc ccagcgcgcc gtgttcgagg
      241 atttcgccgc cagcgtcggt cacgccgagg tctcgcccgg gctgttcggc atcctcatta
      301 tcgtcgaggc caaccccggt ctcaagcagt cccagctcgc cgccgccgcg cacctggatc
      361 gctccagcct ggtgccggtg ctggacaagc tcgaggcgcg cgggcatgtg acgcgtcgcc
      421 cttcgccgga tgaccggcgc gtgaacgggc tgtggctcac cccggccggc gcggcgctgc
      481 tgcgcacgct caagcgcaag gtgctggcgc acgagcagcg cgtgatgcgc ggcatgagcg
      541 agacccagcg cgcgcagctt ttcgatctgc tcgcccgcgt caccccggag acctgacgcc
      601 cgcaccggtc attcgacgtc acgtgcgcca cgcgtgcgct tgacctgcgc gcgcaggcgc
      661 ttctcgcgca gccggcgctc gcgggccgct gcgctcggct tggtcgcctt gcgcggcttc
      721 ggcggcacgc tcgcttggcg gatcagcccg aacaagcgct cgcgcgcgtc gtcgcggttg
      781 cgcacctgcg tgcggtagcg atgcgcctcg atcaccagca cgcccgcggc ggtgagccgc
      841 cgcccggcaa gccggcgcag gcgctcgcgc acgtcctcgg gcagcgaggg cgagccggcg
      901 acgtcgaagc gcagctgcac cgcggaggcg accttgttca cgttctgccc gccgggaccg
      961 ggcgcgcgca cgaacgtctc ctgcagttcc gactcctgca acgacacgcg cgcggagacc
     1021 cgcactgcgc tagccatggc aactccagtt cagtgcgagg tttgtgcttt gcagcatcgg
     1081 cagaatctgc cgcagcatgc gcaacctgcc gcacgcgcaa ctccgcgtgc gacgggcctt
     1141 tgcagcgcac gcttcccctt gcaaacaccg acgactagga ctaaattgcc gagtggattg
     1201 ctgcgccggg tttctgtccc gaacaattcc acaaggaagc ttgcgcagcg atctccacat
     1261 cttcgaccag agacgtccga gaggaccaag ttcaaaccat aactccgatt tgggagagac
     1321 gcatgaatcg atggaagact caactcgccg gggtcgcgaa ggcgatgtcg gccgcagcct
     1381 tggtgggctt cgcggcgcaa gccggcgccg ccgaaatcac ctgggatcgg ctgctcaacg
     1441 ccgaccggga ccccaacaac tggctcatgt atcacggctc gttcaagggc tggcactaca
     1501 gcgggctgga tcagatcaac aagaacaacg tcaagaacct gggcatcgcc tggattcaca
     1561 cgccctccgc ctccaagcgc ggcatccagt cgttcccgct cgtcgccgac ggcattctct
     1621 actacaccag ctccaccggc caggtgtggg cgctggacgc cgccaacggc gcattcgtct
     1681 ggaagtacac cgccaagatc gaccaggagc gtgccgaagg caccttctac aacccctaca
     1741 gccgcggtct cgcggtcggc tacggcaagg tctacgtcgg cacggtggac ggccgcatga
     1801 tcgccctgga tcagaagacc ggccaggtgg tgtgggacaa catgatcctc acggtcgaga
     1861 agggcaacaa gggcttcacc ggcgctccgg tgatcgtgaa ggacaaggtg ctgatcggct
     1921 ccaacggcgg cgagctgtcc ggctgctgcg gcccgatctt cgcggtcaac gcgcagaccg
     1981 gtgaagtcga gtggcagttc gacaccatcg gcggcgacga gcgttcgcgc cagtcttggg
     2041 gcaacgactc ctggaaagtg ggcggcggcg gcggctggat gaccggcagc tacgacgcca
     2101 agaccaacag cgtgtggtgg ggcaccgcca acccggcccc ggactacgac tgggcgggcg
     2161 agaactggat gacggaaggt ccgcgtccgg gcaccaacct gtacagctcc tcggtggtcg
     2221 tgctggacgc ggacaccggc aagctgaagg cctacttcgc ggagatgccg cacgacgcgt
     2281 gggacttcga cagcgcggtg ggcgagttcg tgcagatcga ccgcggcggc aagcgctaca
     2341 tggtgcaccc gaacaagggc ggcgtgatgt tcgtgtacaa cgccgatccc gaccagaccc
     2401 agggcgacca ggtgctcaag gtcgagaacg cctacatgat cggcaagacc tacaactaca
     2461 tcaagggcgt caatcccaag accggcgagc tgatcggccg gcgcgagctg ccgctcggca
     2521 agcacaccaa cgtgtgcccg gcgatcgacg gcgcgatctc ctggaacacc ggcgcgtaca
     2581 acccgaacaa cgggctgtac tacaaggtca cgcaggagtg gtgcttcgac atcgaagtgc
     2641 agaaggtgga ccgtccggcg gacttctccg gtcaggccta cttcggcgcg tcgtggaccg
     2701 cgcgtcaccc cgagggccgg caggccttcg gcacggtgca ggccttcgac cccgtcacca
     2761 gcaagaagag gtgggaaatc gaactgaagt acccgccgct ggcgtcgctg ctcaccacca
     2821 agggcggcct ggtgttcgtg ccgggtgccg acggcacctt ctatgcgctg gacgccgaca
     2881 acggttccaa gctgtggcag cataacaacg gcatcggcca tcacggtggc gtcatctcgt
     2941 acaccgccaa gggcaagcag tacgtcgcag tggtgaccgg atggggcagc cacgtgtccg
     3001 gcaactacgg tccgctgttc ggcgagcctt tcgccagcat gccgaccgac gccggccagc
     3061 tgctggtgtt cagcgtcaag taaccggccc ggccggccga tccggccagg ccagcggttg
     3121 gaccggggcg gggctcaacc ccgccccgtt ttcgtttcca ggttgcagca ccacggttcc
     3181 gattccgagt catgaaacct ctcatcctga tggcgctggc ggtggcctgc ggcgcgccca
     3241 tggcggcatc cgccgacgac aaggacagcg atgcgatcaa gaaactgttc gcgaagaact
     3301 gcgcgctgtg ccatttcaac tacggcctcg accccggccg tggcgccccg aagctcgccg
     3361 gcacacagct gtccctgaag ggcgtctacg accgcatcgc gaacggcaag caaggcgtca
     3421 tgcccggctt caagaagctg ctgaccgagc aggagatcca ggcgctcgcc gactacatca
     3481 aggcgttgcc ggccaactga ctcaccccgc gaaggcaccg tccgcgctgc gcacgcgcgc
     3541 tgtaaactta cgccgcggcg gcgcgttaag aagcgcggcc tccgggtctg ctgccgatgg
     3601 ggcgcagtgg gaccagtcgc gtcggagtaa ggatttcaac gatgagcaac atactcagga
     3661 gcacgctgcc ggcgtatgcc atggtggccg ccttgtcgtc gaccgccacc gcgctggcgc
     3721 agacccaaga catcgatacg gccatcgccc ctgccgtgca gcaggtcaac gagaagtaca
     3781 gcgaacacaa tctggatatc aagaagctat tcgcacagaa atgcagctgg tgccaccagg
     3841 gctacggcat gcgccaggcg gacgggccga agctcgccgg caccgacaag accaaggagc
     3901 agctcatgaa gcagatcgcc aacggcaagt cgccgatgcc cggcttcaag agccagctca
     3961 aggaacacga gcttcaggcg ctggccgagt acatcaaggc cctgccgaag aactgaacgc
     4021 cgcgcttgca ccacgttgcg ctccggcggg tggttcttaa gtcgcgcctt aagggcgcac
     4081 tcggctcagc tcgaagcgca cgccgaaccc ctcgtacttg cccatcccct tcatctgatc
     4141 gcccctgtcg ttgagcttca gggcggcggt catcggtccg ccgccgccat cggcccgaaa
     4201 cttgaaggtg ctgccgtcga aggcgaccgc ggacagcggc ttgggctcct cgccgaacga
     4261 ctcggcgttg gtgagctgca tgctgccgcc gccgtcccgg atgtggaagg tggcccggcc
     4321 gctggacatg ccgcggtacc aggggccctc ccaggtgccg tcgaccgccg cggtgtcggc
     4381 ggcgtgcgtt cgccacgcca ctgccgccat ggccagcatc agcgcaacgc ccagcatgcg
     4441 cggcagattc cgtctcgtca tcgaaacttc agtgcggttg tcgactcgac gacagtgtaa
     4501 acgcagcggg cgccgcttcg ttggcggccg ctgcttgcag ggcgtggtgc ggtaatttct
     4561 ttga
//
LOCUS       DASPGE010000372         4065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1150836, whole genome shotgun sequence.
ACCESSION   DASPGE010000372 DASPGE010000000
VERSION     DASPGE010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150836"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..1514)
                     /locus_tag="VD791_12020"
     CDS             complement(243..1514)
                     /locus_tag="VD791_12020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HXV08751.1"
                     /translation="MRIEILCTGDEVLTGATVNTNFSHISQKLGDAGLEVRWGTTVGD
                     DRETLLQAFRLAAGRADAVIVNGGLGPTVDDLSQEVAAQAAGVELVFSEEWFARMADL
                     FRRRSRVMPPSNRKQAMLPAGAELLDNPIGTACGFALDIGKARFYFTPGVPRELHRML
                     AEQIIPRLLARSGRQTAGFVKRFHSYGIGESRADTLLAGVETLVPDGSVKLGFRAHYP
                     QLETKLAVRGADMDEVRRKLAPVESEVRKRLGNFILAEDDQKLEGVILEELARRQATL
                     ALVETFTGGAVAGRIAPLPGAEKVFRRGVVARDVAEVCDALSLDASAATREVNLETAQ
                     AVARAARRQAGVTHALAALIALDEGDDRMELGGTICLAIAAGEEGATRRARIVGGREW
                     VRLGAVEMALDCLRRYLQGLPVDERIDFEKV"
     gene            1631..3433
                     /locus_tag="VD791_12025"
     CDS             1631..3433
                     /locus_tag="VD791_12025"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HXV08752.1"
                     /translation="MAAKGRDPAMLRSRKWFANPDNPDMTALYLERYMNFGLKREELQ
                     AGKPIIGIAQSGSDLSPCNRHHLELAHRVREGIREAGGIAFEFPVHPIQETGKRPTAS
                     LDRNLAYLGLVEVLYGYFIDGVVLTTGCDKTTPSQIMAAATVDIPAIVLSGGPMLNGW
                     FRGDRTGSGTIVWRAREMLAAGEINGEQFIELIASSAPSAGHCNTMGTALTMNSLAEA
                     LGMSLPGCAAPPAPHKERAVMAYETGKRIVEMVWEDLRPSQILTREAFENAIAVNSAI
                     GGSTNAPIHLNAIARHIGVELDNDDWERIGYDIPLLVNLQPAGEYLGEEYHRAGGVPA
                     VVNELLKAGKIRKSALTVNGKTLAENCAQWPVLDPKVIRPYSQPLKEKAGFLNMKGNL
                     FDSAIMKTSVISSAFRERYLSNPKDPNAFEGRAIVFDGPEDYHHRIDDPALKIDETCI
                     LFIRGTGPKGYPGAAEVVNMRAPAALLKKGVMELPCIGDGRQSGTSGSPSILNASPEA
                     ANNGGLAILKTGDRVRIDLNKRSADILIGAEEVERRRAALAQQGGYKIPEHQTPWQEI
                     QRGMVDELSHGMVLKPAVKYQRIAVSKGVPRDNH"
     gene            3548..>4065
                     /locus_tag="VD791_12030"
     CDS             3548..>4065
                     /locus_tag="VD791_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08753.1"
                     /translation="MPTPAKEEIMANTKTGVNLGRWLAFIGLALAVLCVLAAMASGLG
                     YRMDWWHFRTGFLILRVAFFAALAAAALSLLGLILSRGARGTLIPGLAGLIVALGFAY
                     VPWQWKQTVDALPYIHDITTDVDNPPQFVAAATLRKEGDHPVEYDGPEVAAQQQEAYP
                     DLVPMVVNAPRE"
BASE COUNT          672 a         1431 c         1366 g          596 t
ORIGIN      
        1 gtggcgccgc aggcgccggg gcggtgtggt gcagaagcat cgctgaacca ccccgtcacg
       61 cttcgcgtgc caccccgccc agggcgctct caaggcgcga ttcaaactac ctgctccccg
      121 cctctcacga ggcggggtgg cgccgcaggc gccggggcgg tgtggtgcag aagcatcgct
      181 gaaccacccc gtcacgcttc gcgtgccacc cctcctcgaa gaggagggga atctgctcaa
      241 cgctacacct tctcgaagtc gatccgctcg tccaccggca ggccctgcag atagcgccgc
      301 aggcagtcca gcgccatctc gaccgcaccc aggcgcaccc actcgcggcc gccgacgatg
      361 cgggcgcggc gggtggcgcc ctcctctccc gcggcgatcg ccaggcagat ggtccctccc
      421 agctccatgc ggtcgtcacc ttcgtcgagc gcgatcagcg ccgcgagcgc gtgcgtgacg
      481 ccggcctgcc gccgtgccgc gcgcgccacc gcctgggcgg tttccagatt cacctcgcgc
      541 gtggcggcgg atgcgtccag gctcagcgca tcgcagactt cggcgacatc gcgcgcaaca
      601 acgccccgcc gaaacacctt ctccgcgccg ggcagcggcg cgatgcgccc ggcgaccgcg
      661 ccgccggtga aagtctccac cagcgcgagc gtcgcctgcc gccgggccag ctcctccagg
      721 atcacgccct cgagcttctg gtcgtcctcg gccaggatga agttgcccag gcgcttgcgc
      781 acttcgcttt cgaccggcgc cagcttgcgg cggacttcgt ccatgtccgc gccgcgcacg
      841 gcgagcttgg tctcgagctg cggatagtgc gcccggaaac cgagcttcac gctgccgtcg
      901 ggcaccagcg tttcgacgcc cgccagcagc gtgtcggcgc gcgattcgcc gatcccgtac
      961 gaatggaacc gctttacgaa gccggccgtc tgccggccgc tcctggcgag cagccgcggg
     1021 atgatctgct ccgcgagcat ccggtgcagc tcgcgcggca cgcccggagt gaagtagaag
     1081 cgcgccttgc cgatgtccag cgcgaagccg caggcggtgc cgatgggatt gtcgagcagc
     1141 tccgcgcccg ccggcagcat cgcctgtttg cggttgctcg gcggcatcac ccgactgcgc
     1201 cggcggaaca ggtccgccat cctggcgaac cattcctcgc tgaacaccag ctcgacgccg
     1261 gctgcttggg cggccacctc ctgcgacaga tcgtccacgg tgggccccag cccgccgttg
     1321 acgatcacgg catccgcacg ccccgcggcc agccggaacg cttgcagcag cgtctcgcga
     1381 tcgtcgccga cggtggtgcc ccagcgcact tccagcccgg catcgccgag cttctggctg
     1441 atgtggctga agttggtgtt gacggtggcg ccggtcagga cctcgtcgcc ggtgcagagg
     1501 atctcgatac gcatttgcgg cgagcctagc acaacccacc ccgcggcaac acgcgccggt
     1561 gactgacgcg ggccgaccgg ttaagctatc ggtttcggac gagacgacgc acggacggag
     1621 gagcttcatc atggcagcga aaggcaggga cccggcgatg ctgaggtcgc ggaagtggtt
     1681 cgccaacccc gacaaccccg acatgacggc gctgtatctc gagcgctaca tgaacttcgg
     1741 gctgaagcgc gaggagttgc aggcgggcaa gccgatcatc ggcatcgccc agtcgggctc
     1801 cgatctgtcg ccctgcaacc ggcaccacct ggaactggcg caccgcgtgc gcgagggtat
     1861 ccgcgaggcc ggcggcatcg ccttcgagtt tcccgtgcac ccgatccagg agaccggcaa
     1921 acgccccacc gcctcgctcg accgcaacct cgcctatctg ggcctggtcg aggtcttgta
     1981 cggctacttc atcgacggcg tggtgctcac caccggctgc gacaagacca cgccttcgca
     2041 gatcatggca gccgccaccg tggacatccc ggcaatcgtg ctgtcgggcg ggcccatgct
     2101 caacggctgg ttccgcgggg accgcaccgg ctcgggcacc atcgtctggc gcgcgcgcga
     2161 gatgctcgcc gccggcgaga tcaacgggga gcagttcatc gagctgatcg cctcctcggc
     2221 gccctcggcg ggacattgca acaccatggg cacggccctc accatgaact cgctggccga
     2281 agcgctcggc atgtcgttgc ccggctgcgc ggcacccccg gctccgcaca aggagcgcgc
     2341 cgtgatggcc tacgagaccg gcaagcgcat cgtcgagatg gtgtgggagg acctgcgccc
     2401 gtcgcagatc ctgacgcgcg aggcgttcga gaacgcgatc gcagtgaact cggcgatcgg
     2461 cggttccacc aacgcaccga tccacttgaa cgccatcgcg cgccacatcg gcgtggagct
     2521 cgacaacgac gactgggagc ggatcggtta cgacattccg ctgctggtca acctgcagcc
     2581 ggccggcgag tacctgggcg aggagtatca ccgcgccggg ggcgtgccgg cggtggtcaa
     2641 cgagctgttg aaggcgggca agatccgcaa gtctgcgctc accgtcaacg gcaagacgct
     2701 ggccgagaat tgcgcacagt ggccggtgct ggaccccaag gtgatccggc cctacagcca
     2761 gccgctcaag gagaaggccg gcttcctcaa catgaagggc aacctgttcg acagcgccat
     2821 catgaagacc agcgtgatct cgtcggcgtt ccgcgagcgc tacctgtcca acccgaagga
     2881 cccgaacgcc ttcgaaggcc gcgccatcgt gttcgacggg ccggaggact accatcaccg
     2941 catcgacgac ccggcgctga agatcgacga gacctgcatc ctgttcatcc gcggcaccgg
     3001 ccccaaaggc tacccgggcg cggcggaggt ggtgaacatg cgcgcgcccg cggcgctgct
     3061 caagaagggc gtgatggagc tgccctgcat cggcgacggg cgccagtcgg gcacctcggg
     3121 ctcgccgtcg atcctgaacg catcgccgga agcggccaac aacggcggcc tggcgatcct
     3181 gaagaccggc gaccgggtgc gcatcgactt gaacaagcgc tcggcggaca tcctgatcgg
     3241 cgccgaggag gtcgagcgcc gccgcgccgc gctcgcgcag cagggcggct acaagatccc
     3301 cgagcaccag accccgtggc aggagatcca gcgcggcatg gtggacgagc tgtcgcacgg
     3361 gatggtgctg aagccggcgg tgaagtacca gcgcatcgcc gtcagcaagg gggtgccgcg
     3421 cgacaatcac tgacgacccc ctctccccac agacaaagag gtccctctcc cgcttgcggg
     3481 agagggtagg gtgagggccg gggtgagggg tagcggcgca gccgtcgtca ggatgcagcg
     3541 caatccgatg ccaacacctg caaaggagga gatcatggcg aataccaaga ccggcgtgaa
     3601 tctcggcaga tggctcgctt tcatcggcct ggcgctcgcg gtgctgtgcg tgctggcggc
     3661 gatggcctcg ggcctgggct atcgcatgga ctggtggcac ttccgcaccg gcttcctgat
     3721 cctgcgcgtg gcgttcttcg cggcgctggc ggccgcagcg ctgtcgctgc tggggctgat
     3781 cctgagccgg ggcgcgcgcg gcacgctgat ccccgggctg gccgggctga tcgttgcgct
     3841 ggggttcgcc tacgtgccgt ggcaatggaa gcagacggtc gacgcgctgc cctacatcca
     3901 cgacatcacc accgacgtcg acaacccgcc gcagttcgtc gcagcagcga cgctgcgcaa
     3961 ggagggcgat cacccggtgg agtacgacgg gcccgaagtg gcggcccagc agcaggaagc
     4021 ctatccggat ctggtgccga tggtggtgaa cgctccgcgc gagaa
//
LOCUS       DASPGE010000373         6489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1150917, whole genome shotgun sequence.
ACCESSION   DASPGE010000373 DASPGE010000000
VERSION     DASPGE010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150917"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..931)
                     /locus_tag="VD791_12035"
     CDS             complement(14..931)
                     /locus_tag="VD791_12035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HXV08754.1"
                     /translation="MTTSARAQWLAVLNPVSGGGRGVRQRARIERLLRECGIDYRLLV
                     SEHAGQAIALARGAAHSGCRRFVCIGGDGTLNEVLNGALEAGAGETTLALIPVGRGDD
                     WARTFGIPRDFEAAVRLIAAGHTRQQDVGVARFEHGGSAVQRHFINVAGVGFDAYVVQ
                     RTRVLRLGPLTYLAGLLQGFLTFRAPRLRVTAGAFVRDEKMFLAFAAVGRYCGGGMQV
                     APQAIVDDGEFDVVTVGEVTKGELMVNLRRLFDGTLPEYAKVKTARAAQVRVEAVPPA
                     QVEADGELLGQTPVTFSVLPRAVRVVVPE"
     gene            1116..1418
                     /locus_tag="VD791_12040"
     CDS             1116..1418
                     /locus_tag="VD791_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08755.1"
                     /translation="MGSRAFSILTRKARPILNGRDYDNAKKRLAAAMQQPGWVREDER
                     IDALTVALNEFETRYVARELQRAVEWAECVFVPSIDNPDSPRRRWTDPQDSPPVRP"
     gene            1571..2551
                     /gene="dusA"
                     /locus_tag="VD791_12045"
     CDS             1571..2551
                     /gene="dusA"
                     /locus_tag="VD791_12045"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HXV08756.1"
                     /translation="MEAASHRLCTAPMMDWSDRHCRYFFRQLSSHATVYSEMVTAGAL
                     LHGNLERHLRFDPAEHRLALQLGGSEPEELARCARLGRDWGYDEINLNCGCPSERVQR
                     GAFGACLMAEPQLVADCVKAMQDAVDVPVTVKHRLGIDRIEEHEFVHRFVETLARAGC
                     RTFIVHARNAVLKGLSPRDNRAVPPLRYAAVYRLKHEFPALDIVINGGIQSQAEITGH
                     LAHLDGVMLGRAAYHDPYCLARADAALWGGVPATRIAVGEAMYRYASRETTAGTPLRA
                     IARHLLGLFHGEPRARLWRRMLSDAAALACNDPGLILRALEATEPACAAA"
     gene            2752..3411
                     /locus_tag="VD791_12050"
     CDS             2752..3411
                     /locus_tag="VD791_12050"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HXV08757.1"
                     /translation="MQSRFGRLAAHFFGGPAPGGTPGAAEPAAARELPQPRERWITEA
                     SPQLLGEERTQDFLVVTFCAAFSLIGWLMIVQGADQIQRARQAAQWPSTLGTVESTEV
                     YAVEGSQGAHWRPQVTYSYAVHGQLVTATRVSLGKARFFQDAQRAQAYAARYPARSEV
                     TVFYNPAEVTESVLEIDTPRSVHANVAFGVTLACLGPMLLALFGFPRYRGRRRAAAFG
                     A"
     gene            3389..4081
                     /locus_tag="VD791_12055"
     CDS             3389..4081
                     /locus_tag="VD791_12055"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HXV08758.1"
                     /translation="MRRHSARSGPQSRTERVWDLPVRLFHWVLVGLIATSWITAQIGG
                     NAMQVHQWSGMSILTLVLFRVLWGFLGSTRARFSDFVRGPRAAFAYARSLLRGGAAFH
                     PGHNPLGGWMVLALLTSLLLQAGTGLFANDDIMLEGPLAKHISKEASDWLTGIHHLNF
                     AVLLALSALHVAAALFYLLVKRDNLMLPMITGRKPLPEGRAFDPPRGGPPWLAALLLG
                     LCAGAVWALVSA"
     gene            complement(4199..4648)
                     /locus_tag="VD791_12060"
     CDS             complement(4199..4648)
                     /locus_tag="VD791_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXV08759.1"
                     /translation="MKRSSIAALIGAVAFAGGSALAQQKPEDAIKYRQSALFIMGQHF
                     GPLAGMAQNKIPYDKDAAVRHAAMVEFMSKLPWDRFAAGTDQGNTKAKPEIWLNLEDF
                     KAKAEKMQGEVAKLAQVSKAGDLNALKTQVGETGKACKACHDEYRNK"
     gene            complement(4690..5046)
                     /locus_tag="VD791_12065"
     CDS             complement(4690..5046)
                     /locus_tag="VD791_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08760.1"
                     /translation="MTRSFAGCAVLLACVALAGCASPGKQSKAPRGGAAQVQVYNTTQ
                     LVPSQYTVVQRLWIDGWRSNLTYPTYRSVENGIEAMREKAADAGANGLLNVMCMDASG
                     YSKGRLLCYGDAIKFN"
     gene            complement(5131..5805)
                     /gene="otnC"
                     /locus_tag="VD791_12070"
     CDS             complement(5131..5805)
                     /gene="otnC"
                     /locus_tag="VD791_12070"
                     /EC_number="4.1.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate 4-phosphate decarboxylase"
                     /protein_id="HXV08761.1"
                     /translation="MTEAGLREEICRVGKSLYDRGLTFGSAGNISARVPAGWLMTPTN
                     SCLGRLDPATLSLVDDAGNLLSGDKPTKEAFLHLSMYARRPDAGAVVHLHSTYSVAVS
                     VLADLDPQNPIPPITAYYVMKIGKLALLPYYAPGDASLANAVREVADRHHAVLLANHG
                     PVVAGKDLDTAVYATEELEETARLYLMLRGQALKLLSPEQVAELRRRYPESEPLSRRA
                     PHRGSG"
     gene            complement(5816..>6489)
                     /locus_tag="VD791_12075"
     CDS             complement(5816..>6489)
                     /locus_tag="VD791_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide-binding domain containing protein"
                     /protein_id="HXV08762.1"
                     /translation="ILDSITDQHLHALGEACADLELVTGGSGMAMGLPANFVRAGLLQ
                     PGRQAALPRVTGPTAVLSGSCSVATQGQVAKMKQDHEAFELDPIAIGGGEDVAAQALA
                     WASTRLGEKPILLYSTAAPERVAAVQSQLGREEAGALIERTLGRIAQSLVQQGVRRLV
                     VAGGETSGAVVSALGVQGLAIGPEIDPGVPWTFSIGEPPLALALKSGNFGAPDFFTKA
                     FRVQP"
BASE COUNT          959 a         2345 c         2184 g         1001 t
ORIGIN      
        1 ccgcgccggc gacctactcc ggaacgacca cgcgtaccgc gcgcggcagc acgctgaacg
       61 tcaccggtgt ctgccccaac agctcgccgt cggcctccac ttgcgccggc ggcaccgctt
      121 ccacccgcac ctgcgccgcg cgcgccgtct tcaccttggc gtattcgggc agcgtgccgt
      181 cgaacaggcg ccgtaggttg accatcagct cgcccttggt cacctccccc accgtcacca
      241 cgtcgaactc tccgtcgtcc acgatcgcct gcggcgccac ctgcatgccg ccgccgcaat
      301 agcggccgac ggcggcgaag gcgaggaaca tcttctcgtc gcgcacgaac gcgccggccg
      361 tgaccctgag ccgcggcgca cgaaaggtga gaaagccctg cagcagcccg gccaggtaag
      421 tgagcggacc cagccgcagc acgcgcgtgc gctgcaccac gtaggcgtcg aagccgacgc
      481 ccgccacatt gatgaagtgt cgctggaccg ccgatccgcc gtgctcgaac cgcgccacgc
      541 ccacgtcctg ctgcctggtg tgcccggccg cgatcaaccg caccgcggct tcaaagtcgc
      601 gcggaattcc gaaagtgcgg gcccagtcgt cgccgcggcc caccgggatc agcgccagcg
      661 tcgtctcgcc ggctccggct tccagcgccc catttagcac ttcgttaagg gtgccgtcgc
      721 cgccgatgca gacgaagcgc cggcagccgc tgtgcgccgc gccgcgcgcc agggcgatgg
      781 cctgccccgc gtgctcgctc accagcaggc ggtaatcgat accgcactcg cgcagcagcc
      841 gctcgatgcg cgcgcgctgc cgcacgcctc ggcctccgcc ggacaccgga ttgagcaccg
      901 cgagccactg cgcccgagcg ctggtcgtca tggcgccgca gtatgcatca agggccgcgg
      961 cagcgtcgcc cggcagccgc cctcgcatga cattgccctg acaattctcg acactgccgc
     1021 gcattccccg ttgaatgtcg cctgcggccc acacacgggc ccgcaacgaa atacggtgag
     1081 caggcaacga acacagcaac gggcaggaga ccgggatggg cagcagagcg ttcagcatcc
     1141 tcacgcgcaa ggcgcggccg atactcaacg ggcgcgacta tgacaacgcc aagaagcgcc
     1201 tggctgctgc gatgcagcag ccaggctggg tgcgcgaaga cgagcgcatc gatgcgctga
     1261 ccgtggcgct caacgagttc gagacgcgct acgtcgcgcg cgagctgcag cgcgccgtgg
     1321 agtgggccga gtgcgtcttc gtgccgtcca tcgacaaccc ggacagcccg cgccggcgct
     1381 ggaccgaccc gcaagattcg ccgcctgtgc gcccttgagg cgcgactcga gaaacccctt
     1441 ctcccgcctc tgacggaggc gggagcagca acgccggggc cgagtcaccg gcgcggctcg
     1501 ccgccggcga ctccacgggt ctggcgcagc gcatgcgccc gccccatacg cacctttaca
     1561 atagcggccc atggaggccg cttcgcatcg cctgtgcacg gcgccgatga tggactggtc
     1621 ggaccggcac tgccgctatt tcttccgcca gctgtcctcg catgccacgg tgtacagcga
     1681 aatggtcacc gctggcgcgc tgctgcacgg caatctcgaa cgccatctgc gcttcgaccc
     1741 ggcggagcat cggctcgccc tgcagctggg cggcagcgag cccgaggaac tggcgcgctg
     1801 cgcgcgcctc gggcgcgact ggggctacga cgagatcaac ctgaactgcg gctgcccgtc
     1861 ggagcgcgtc cagcgcggtg cattcggcgc ctgcctgatg gccgagccgc agctcgtggc
     1921 cgactgcgtg aaggcgatgc aggacgcggt ggacgtgccg gtaacggtga agcaccgcct
     1981 gggcatcgat cgcatcgaag agcatgagtt cgtacaccgc ttcgtcgaga cgctggcgcg
     2041 cgccggctgc cgcacgttca tcgtccacgc ccgcaatgcg gtgctgaagg gcctgagccc
     2101 gcgcgacaac cgcgctgttc cgccgctgcg atacgccgcg gtgtaccgcc tcaagcacga
     2161 gttccccgcg ctcgacatcg tgatcaacgg cggcatccag tcccaggcgg aaatcaccgg
     2221 gcacctggcg cacctggacg gcgtcatgct cgggcgggcg gcgtatcacg atccctattg
     2281 cctcgcgcgg gcggacgcgg ccctgtgggg aggtgtgccc gccacgcgca tcgcggtcgg
     2341 cgaggcgatg taccgctacg ccagccgcga aacgaccgcc ggcaccccgc tgcgcgccat
     2401 tgcgcgtcac ctgctggggt tgttccacgg tgagccgcgg gcgcggctct ggcgccggat
     2461 gctgtcggac gccgccgcgc ttgcgtgcaa cgaccccggg ctgatcctgc gcgccttgga
     2521 ggccaccgag ccggcgtgcg cggccgcctg agcgcgctcg ggcgcaccgc cggcgcgatc
     2581 accggcagcg cgtccgagcg ggtttaacaa tggttccgcc ggggcgcgct cgcgactaca
     2641 ctgatcgccg tcgctgagcg ggtttggcgc gcgaaccatg cggggaatcg atccgtccgc
     2701 cccggggccg cgagcgcgcg aaccgcagcc gcctcagccg ggaggaatca gatgcagagc
     2761 cggttcggcc gcctggcggc ccacttcttc ggcggtccgg cccccggcgg cacgccaggc
     2821 gccgccgagc ccgcagcagc gcgcgagttg ccgcagcccc gagagcgctg gatcacggag
     2881 gcgagcccgc agctcctggg cgaggaacgc acccaggatt tcctcgtcgt caccttctgc
     2941 gcggcgttct cgctgatcgg ctggctgatg atcgtgcagg gcgcggacca gatccagcgc
     3001 gcgcgccagg cggcccaatg gccaagcacg ctcggtaccg tggagtcgac ggaggtgtac
     3061 gccgtggaag gctcccaggg cgcgcactgg cgcccgcagg tgacctattc ctacgccgtg
     3121 catggccagc tcgtcaccgc cacgcgcgtg tcgctcggca aggcacgctt cttccaggac
     3181 gcgcagcgtg cgcaggccta cgccgcgcgc tacccggcac gcagcgaggt gaccgtgttc
     3241 tacaacccgg ccgaggtgac cgagtcggtg ctcgagatcg acaccccgcg cagcgttcac
     3301 gccaacgtcg cgttcggcgt gacgctcgcc tgcctggggc cgatgctgct ggcgctgttc
     3361 ggctttccgc gctaccgggg acgccgccgt gcggcggcat tcggcgcgta gcgggccaca
     3421 atcgcgcacg gagcgggtat gggacctgcc cgtgcgtttg ttccactggg tcctggtcgg
     3481 gctgatcgcg acctcgtgga tcaccgccca gatcggcggc aacgccatgc aggttcacca
     3541 gtggagcggc atgtcgatcc tgacgctggt gctgttccgc gtgctgtggg ggtttctcgg
     3601 cagcactcgg gcgcgcttct cggacttcgt gcgcggtccg cgcgcggctt tcgcctacgc
     3661 ccgcagcctg ctacgcggcg gcgccgcctt ccaccccggc cacaacccgc tgggcggctg
     3721 gatggtgctc gcgctgctga caagcctgct gctgcaagcc ggcaccggtt tgttcgccaa
     3781 cgacgacatc atgctcgagg gaccgctggc gaagcacatc tccaaggagg cgagcgactg
     3841 gctgaccggc atccaccacc tcaacttcgc cgtcctgctg gcgctgagcg cgctgcacgt
     3901 ggcggccgcg ctgttctacc tgctggtcaa gcgcgacaac ctgatgctgc cgatgatcac
     3961 cggacgcaag ccgctccccg agggccgcgc attcgacccg ccgcgcggcg ggccgccgtg
     4021 gctcgccgcg ctgctgctgg ggctgtgtgc cggggcggtg tgggcgctgg tcagcgcgtg
     4081 actggagcat gtgccggagc agccgggaga tgcaacagcc cctctttctc cccggattcc
     4141 gcttcgccgc atccgggcca cgccccggag tcacgaatca cgattcacga atcacgcctt
     4201 acttgttccg atactcgtcg tgacaggcct tgcaggcctt gccggtctcg cccacctggg
     4261 ttttgagcgc gttcaagtcg ccggccttgg acacctgggc gagcttggcg acctcgccct
     4321 gcatcttctc ggccttggcc ttgaagtcct cgagattgag ccagatctcc ggcttcgcct
     4381 tggtgttgcc ctgatcggtg cccgccgcga agcggtccca cggcagcttc gacatgaact
     4441 cgaccatcgc cgcgtggcgc accgctgcgt ccttgtcgta ggggatcttg ttctgtgcca
     4501 tgcccgccag cgggccgaag tgctgaccca tgatgaacag ggcggactgc cgatacttga
     4561 tcgcgtcctc gggcttctgc tgggccagcg cgctgccgcc ggcgaacgcg accgcgccga
     4621 tcagcgctgc gatgctgctg cgtttcatgt ctcctcctcc gcgtggggat cggtgcccct
     4681 tggttgaact cagttgaatt ttatggcgtc gccgtagcac agcagccggc ccttgctgta
     4741 gccgctcgcg tccatgcaca tgacattgag caggccgttg gcgccggcgt cggccgcctt
     4801 ctcgcgcatc gcctcgatgc cgttctccac cgagcggtag gtgggatagg tgaggttgct
     4861 gcgccacccg tcgatccaca gccgctgcac caccgtgtac tggctgggaa cgagctgcgt
     4921 ggtgttgtac acctgcacct gcgccgcccc gccgcggggc gccttggatt gcttgccggg
     4981 actcgcgcag ccggcgaggg ccacgcacgc caacagcacg gcacaacctg cgaaggaacg
     5041 cgtcatgaac gatctccagg ggttgaaagg gccaagagtt tgagggccaa gggttgaaaa
     5101 gggctcggcg tacgctaacc caattccggg ctatccgctc ccgcgatggg gggcacgccg
     5161 ggagaggggc tccgattcgg ggtatctgcg ccgcagttcg gcaacctgct cgggcgaaag
     5221 caacttgagg gcctgcccgc gcagcatcag gtaaagcctg gcggtctcct ccagctcctc
     5281 ggtggcgtag accgcggtgt cgaggtcctt gcccgccacc accgggccgt ggttggccag
     5341 cagcaccgcg tggtggcggt cggccacctc gcgcacggcg ttggccaggc tcgcatcgcc
     5401 gggcgcgtag tagggcagca gcgcgagctt gccgatcttc atcacgtaat aggcggtgat
     5461 cggcgggatc gggttctgcg gatcgaggtc ggccagcacg gacacggcga ccgaatacgt
     5521 cgagtgcagg tgcacgacgg cgccggcgtc gggccggcgg gcgtacatcg acaggtgcag
     5581 gaaagcttcc ttggtgggct tgtcgcccga cagcaggttg cccgcgtcgt cgaccaggga
     5641 cagcgtggcc gggtcgaggc ggccgagaca ggaattggtg ggcgtcatca gccatcccgc
     5701 cggcacccgt gcgctgatgt tgcccgcgct gccgaaggtc aggccgcggt cgtacagcga
     5761 cttgccgacg cggcagatct cctcgcgcaa tccggcttcg gtcatcgagc gcacctcacg
     5821 gctgcacgcg gaacgccttg gtgaagaaat ccggcgcgcc gaaattcccg gacttgagcg
     5881 ccagcgccag cggcggctcg ccgatgctga aggtccaggg cacgcccggg tcgatctcgg
     5941 ggccgatggc caacccctgc accccgagcg cgctcaccac cgcgccggaa gtctcgccgc
     6001 cggccaccac cagccgccgt accccctgct gcaccaggct ctgcgcgatg cgtcccagcg
     6061 tgcgctcgat cagcgcgccc gcctcttccc gtcccagctg actctgcacc gctgccaccc
     6121 gctcgggcgc ggcggtggag tacagcagaa tcggtttctc accgagccgc gtggaagccc
     6181 acgccagcgc ctgcgcggct acgtcctcgc cgccgccgat ggcgatcggg tccagctcga
     6241 aggcctcgtg gtcctgcttc atcttcgcca cctggccctg cgtggccacc gagcaactgc
     6301 cggagagcac cgccgtgggg ccggtaaccc tgggcagcgc cgcctgccgg ccgggctgca
     6361 gcagccccgc gcgcacgaag ttggcgggca gtcccatcgc catgccggag ccgccggtga
     6421 cgagctcgag gtccgcacac gcctcgccga gcgcgtgcag atgctggtcg gtgatcgagt
     6481 cgaggatcg
//
LOCUS       DASPGE010000374         3980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1737763, whole genome shotgun sequence.
ACCESSION   DASPGE010000374 DASPGE010000000
VERSION     DASPGE010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737763"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VD791_12080"
     CDS             complement(<1..364)
                     /locus_tag="VD791_12080"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08763.1"
                     /translation="MHPLLKEIRHNPLLWLLAFVPVVFIARELDPEGHTLLFVLSVLA
                     IVPLAALLSHATESVAAKTGDTVGGLLNATLGNLTELVIALAALRAGQSTLVTASIAG
                     AIVTDALLVLGMSFLLGGL"
     gene            675..1223
                     /locus_tag="VD791_12085"
     CDS             675..1223
                     /locus_tag="VD791_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HXV08764.1"
                     /translation="MRRGAGAALWVLAWLPGAGQASFLSGDMLDAAANVIALIVLLVV
                     PVAVVVVFWLVHILPEKIAERKHHPQKDAIKTLCLLSLAFGGLLWPLAWVWAYTKPVL
                     HKLSYGTDKHEDYYRELAEHDMAEARVLKDDVVRLRRELELLQVRGGGLPDELAEIHE
                     QLAVVEARLPAEPPVLSKAVAK"
     gene            1224..2447
                     /locus_tag="VD791_12090"
     CDS             1224..2447
                     /locus_tag="VD791_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HXV08765.1"
                     /translation="MEVLLLGIYAFFVWLIFIKLKWLPWNITSQVIVVIIPILGLTAL
                     ILLLNVYAPSSSDVRVYKYTVSIVSQVRGRVLEVPVEEGNRPVKKGDVLFKIDPTPYQ
                     LTVSQLEAQLANAEASQRELDESLRGAKAQVVETRSAIQQATSRTREVAAKLDLARMR
                     VQQHAELVKTGAGSKFDLERAQTDLAEVQAQLDTARGVEAQARAAEVQASASVQQIQQ
                     KLSAKIDGEYAQVATVRAQLDTARWELEQTVVRSPCDCNIVNLQLRPGAFVAGIPLNA
                     VMTLVEVEGQVVALFNQNELHQVQSGDEAEFALNTYPGKIIKAKVDSIIWAQGQGQLP
                     ASGTLPMTGVLTQPPGRFAVKFDIAERDRALFLAAGAAGDAAIYTQRLAMIHIIRKVI
                     LRVGSYVNYLIPKLH"
     gene            2455..>3980
                     /locus_tag="VD791_12095"
     CDS             2455..>3980
                     /locus_tag="VD791_12095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXV08766.1"
                     /translation="MRPSRAVAALLLSALAGCALKAPPDPVELRRQALPNLQVPERWA
                     SEGALGASVAEGWLATFGDPVLIVLAEEALAYNADLRVAGTRVEQAAGYASVAAAGLY
                     PAAYLLARGGGKLSGDNSGLQGVGLTANWEVDVWGRVRYGRKAADAQYASALADLEYA
                     RQSLVAMVAKSWTLAIEARLQRAVAQDTVRSAEQLANLARDRERVGRGDAMEVANAEA
                     NVETYRDALRQLELSYRQSVRALEVLLGRYPSAYLEALDALPSMAPPVPAGLPSELLE
                     RRPDVIAAERRVAAAFNRVGEAQTARLPRISLTAGVNSISSDLFVLQERDNPVWSAGA
                     NLLAPIYQGGALKAQVEIRTAEQRQAVAEYAQLALRAFSDVESALATEFAAREREEIL
                     TRAVTQWERAVGLARVAYEVGSIDLRPVQQQQLGAFAARSALLRVQSEQRVQRVNLHL
                     ALGGGFGGPDTNLSDRTAVRSEAGASIINVYRVVGSGGAEVKVPASGWPASVKVRLHG
                     "
BASE COUNT          639 a         1314 c         1443 g          584 t
ORIGIN      
        1 tgagcccgcc aaggaggaag gacattccca gtacgagaag agcgtcggtg acgatcgcgc
       61 cggcgatgga tgccgtcacc agcgtggact gcccggcccg cagggcggcc agcgcgatga
      121 ccagctcggt caggttcccc agcgtggcgt tgagcagacc gcccaccgta tcgccggtct
      181 tggcggcaac ggactcggtg gcgtggctca gcagcgcggc cagcggcacg atggccagca
      241 cggacagcac gaacagcagc gtgtgcccct cgggatcgag ctcccgggcg atgaacacca
      301 cgggcacgaa ggccagcagc cagaggagcg ggttgtgacg gatctctttc agcagggggt
      361 gcatgaggca gccaagcata gccgaaggca acgcccgcgg tcgagagtgg ccgcaggatc
      421 ggacgaaggc tttgtcgcga cccgcagcca ccccggcaag cccgcccgcg cggcccggga
      481 gcctgcgctc tggccggata ccgccttctc gtgcatactt gagcgcattg gcgcgcggcc
      541 gtctgccggc agcactggcg gtgtcggacg gacgcgggga ggaggaagcg atgagatcgt
      601 ggattgcgcg tcgcgccgga cgacgcgtca aacaaacgtt tcaagacgtg ctgcccgccg
      661 ctggacggtg cgcggtgcga cgcggcgccg gcgccgcgct gtgggtcctg gcatggctcc
      721 cgggcgcggg ccaggcatct ttcctgtccg gcgacatgct cgacgccgcg gcgaacgtca
      781 tcgcactgat cgtgctgctg gtcgtgccgg tcgccgtcgt ggtggtcttc tggctggtgc
      841 acatccttcc ggagaagatc gccgagcgca aacaccatcc gcagaaggac gcgatcaaga
      901 ccctgtgcct gctgtccctg gcgttcggcg ggctgctgtg gccgctcgcc tgggtctggg
      961 cctacaccaa accggtcctg cacaagctct cttacggcac ggacaagcac gaggactact
     1021 acagggagct ggccgagcac gacatggcgg aagcccgggt gctcaaggat gacgtcgtgc
     1081 gcctgcggcg cgagctggag ctgctgcagg tgcgcggcgg cggactgccg gacgagctgg
     1141 ccgagatcca cgagcagctg gccgtggtgg aagcgcggct gccggcggag ccgcccgtcc
     1201 tttcgaaggc ggtagcgaag tgaatggaag tcctgctcct cggcatctac gcgttcttcg
     1261 tctggctgat cttcatcaag ctcaagtggc tgccgtggaa catcaccagc caggtgatcg
     1321 tggtgatcat cccgatcctc gggctcaccg ctctcatcct gctgctcaac gtctacgcgc
     1381 cgtcctcgtc ggatgtgcgc gtgtataagt acaccgtttc catcgtctcg caggtcaggg
     1441 gccgggtgct ggaagtgccg gtcgaggaag gcaatcgccc ggtgaagaag ggtgatgtgc
     1501 tgttcaagat cgaccccacg ccctaccagc tcaccgtatc ccagctcgaa gcgcagctcg
     1561 ccaacgcgga ggcctcccag cgcgagctgg acgagtcgct gcgcggcgca aaggcccagg
     1621 tggtcgaaac gcgcagcgcc atccagcagg ccacctcgcg cacgcgcgag gtggcggcga
     1681 agctggatct cgcacgcatg cgcgtgcaac agcacgccga actggtcaag accggggcgg
     1741 gcagcaagtt cgatctggaa cgggcgcaga ccgatctcgc cgaagtgcag gcgcaactgg
     1801 acaccgcccg gggagtggag gcgcaggcgc gcgccgccga ggtgcaggcg agcgcctcgg
     1861 tgcaacagat ccagcagaag ctcagcgcca agatcgacgg cgagtacgcg caggtcgcca
     1921 cggtgcgcgc gcagctcgac accgcccgct gggagctgga gcagaccgtg gtgcgctcgc
     1981 cctgcgactg caacatcgtc aacctgcagc tgcgaccggg ggccttcgtt gccggcatcc
     2041 cgctcaatgc cgtcatgacg ctggtcgagg tggaaggcca ggtggtcgcg ctgttcaacc
     2101 agaacgagct gcaccaggtg cagtcgggag acgaggccga gttcgcgctc aacacctatc
     2161 ccggcaagat catcaaggca aaagtggact cgatcatctg ggcccagggg caagggcagc
     2221 tgccggcgag cggaaccctg ccgatgaccg gggtgctgac ccagcctccg ggccgcttcg
     2281 ccgtgaagtt cgatatcgcc gagcgcgacc gggcgctgtt cctggcggcg ggggctgcgg
     2341 gcgacgccgc catctacacg cagcgtctgg ccatgatcca catcatccgc aaggtgatcc
     2401 tgcgggtggg ctcgtacgtc aactatctca tccctaagct gcactgagag ggcgatgcga
     2461 ccgagccgcg ccgtggccgc gctcttgctg tcggcgctcg cgggatgcgc cttgaaggcg
     2521 ccgccggatc ccgtcgagct gcgccgtcag gcgctgccca acctgcaggt gcccgaacgc
     2581 tgggcctcgg agggcgcgct gggcgcgtcg gtggcggagg gctggctcgc gactttcggc
     2641 gatccggtgc tgatcgtgct cgccgaggaa gcgctcgcct acaacgccga cctgcgggtc
     2701 gcaggcacgc gcgtcgagca ggcggcgggc tatgcgtcgg tggcggcggc gggcctgtac
     2761 ccggcggcgt atctgctcgc gcgcggcggc ggcaagctga gcggcgacaa ctcggggttg
     2821 cagggcgtgg ggctcaccgc caactgggag gtcgacgtgt gggggcgggt gcgctacggg
     2881 cgcaaggcgg cggacgccca gtacgcctcg gcgctggccg acctcgagta cgcgcggcaa
     2941 tcgctggtcg ccatggtcgc caagagctgg acccttgcca tcgaggccag gttgcagcgc
     3001 gcggtggcgc aggacacggt gcgctcggcc gagcagctgg cgaatctcgc cagagaccgc
     3061 gaacgcgtgg gccgcggcga cgcgatggaa gtcgccaacg ccgaggcgaa cgtggagacc
     3121 taccgcgacg ccctgcgtca gctcgagctg tcttatcgcc aatcggtgcg tgcgctcgag
     3181 gtgttgcttg gccgctaccc atccgcgtac ctcgaggcgc ttgacgcgct gccgtcgatg
     3241 gcgccgccgg tgcccgcagg attgccttcc gagctgctcg agcggcgccc cgacgtgatc
     3301 gccgccgagc gccgcgtagc cgccgcattc aatcgcgtcg gcgaagccca aaccgcgcgg
     3361 ctgccgcgca tctcgcttac cgccggcgtc aacagtatct ccagcgatct gttcgtgttg
     3421 caggagcgcg acaacccggt gtggagcgcc ggcgccaacc tgctcgcgcc gatctatcaa
     3481 ggcggtgcgc tcaaggcgca ggtcgagatc cgcaccgcgg agcagaggca ggcggtggcc
     3541 gagtacgcgc agctcgcgct gcgcgcgttc agcgacgtcg agagcgcgct ggcgaccgag
     3601 ttcgccgcgc gcgagcgcga ggagatcctg acgcgcgcgg tgacgcagtg ggagcgcgcc
     3661 gtcgggttgg cccgcgtcgc ctacgaggtc ggctccatcg acctgcgccc ggtgcagcag
     3721 caacagctgg gcgcgttcgc ggcgcgcagc gcgctgctgc gcgtgcagag cgagcagcgc
     3781 gtgcagcggg tgaacctgca cctggccctc ggcggcggct tcggcggccc cgacaccaat
     3841 ctgagcgatc gcaccgccgt gcgcagtgag gcgggcgctt cgatcatcaa cgtgtaccgc
     3901 gtcgtgggca gcgggggcgc cgaggtcaag gtgccggcga gcggctggcc cgcgagcgtg
     3961 aaggttcggt tgcacggttt
//
LOCUS       DASPGE010000375         5214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1033407, whole genome shotgun sequence.
ACCESSION   DASPGE010000375 DASPGE010000000
VERSION     DASPGE010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033407"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..768
                     /locus_tag="VD791_12100"
     CDS             79..768
                     /locus_tag="VD791_12100"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HXV08767.1"
                     /translation="MRRWCTALASSLLAAGCAAHAAPPGRVVIDYEVRYNGSAMATAQ
                     HVLEHDGKTYRLLETWEGSGLLSLLGEVRRTSEGLVTPEGLRPLVYEDRRPRRDLAMA
                     RFSWKERTLTQQFRGAPRTEPMPPNAQDRLSLVFAPAFKVPGKGPLDFHVADGKGVAH
                     YVFEIAGRERLRVPAGEFDALHLVKRDNDGRATEWWLDAGHAYLPLRVLVVRKDGTRI
                     DQLAARITPAP"
     gene            765..2015
                     /locus_tag="VD791_12105"
     CDS             765..2015
                     /locus_tag="VD791_12105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HXV08768.1"
                     /translation="MIFRAQLARAEALLGELLAQGGPADAAVSRYFRAHRNLGQQDRA
                     FVAETVYAVLRRRRSLAAAAGSDAPRALAIAALVRVRGLSGRALEGVLREDEQALVAR
                     VRAARSETFAPAVRADLPDWLWERLAALHGEDEAMRIAQGLLNPAPLDLRVNLAKAGR
                     DEALARLRADGIEAAPTPHSPAGIRLAGKPAVNRHPLFQGGVIEVQDEGSQLLAYLLA
                     PRRGEMIADYCAGAGGKTLALAMLMRGTGRIYAMDVSSRRLGELAPRAARAGVSNVHA
                     LVLSGEGDARAKRLALKLDRVLVDAPCSGFGTLRRNPDLKWRHDATAIRELAAKQAAI
                     LATAARLVKPGGRLVYATCSILPEENEDVARAFAAAHPEFQPLSCREILAAQRIALDC
                     DEQLRLWPHRHGCDGFFAAAFERR"
     gene            2154..3326
                     /locus_tag="VD791_12110"
     CDS             2154..3326
                     /locus_tag="VD791_12110"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HXV08769.1"
                     /translation="MIFSGLFDLPWWGYVLVALGLTHVTIASVTIFLHRHQAHRALDL
                     HPIASHFFRFWLWLTTGMVTKEWAAIHRKHHAKCETPEDPHSPQVLGINRVLWGGVFL
                     YVKESYKAETMERYGHGTPDDWMERNLYTRHAILGLTLMGAVNLLLFGLIPGALILIT
                     QIVWIPFWAAGVINGIGHYWGYRSFDIADASKNIVPWGILIGGEELHNNHHAYATSAK
                     LSAKWYEFDIGWMYIRLLEALGLATVKKVAPTPRFEPAKTMCDYDTLQAVIANRYDVL
                     ARYAKAIKRTWSDEIERLQHWSPRDAEMLKPLKRALVRIGYGAEADRARVAEALKPSK
                     PLQVAVSMREELIALWERSHASKEQLLRQLQDWCQRAEASGIAPLIDFSRRLRSYA"
     gene            complement(3390..3545)
                     /gene="rpmG"
                     /locus_tag="VD791_12115"
     CDS             complement(3390..3545)
                     /gene="rpmG"
                     /locus_tag="VD791_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HXV08770.1"
                     /translation="MRDKIKLESTAGTGHFYTTTKNKRTTPDKMEIKKYDPKARKHVL
                     YKEAKLK"
     gene            complement(3545..3802)
                     /gene="rpmB"
                     /locus_tag="VD791_12120"
     CDS             complement(3545..3802)
                     /gene="rpmB"
                     /locus_tag="VD791_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HXV08771.1"
                     /translation="MSRVCQVTGKKPMKGNLVSHANNKTRRLFQPNLHYRRFWVESQN
                     RWVRLRVSTAGLRKIDKLGVEAVLAEIRARDKAAAKQKESR"
     gene            complement(3874..4548)
                     /gene="radC"
                     /locus_tag="VD791_12125"
     CDS             complement(3874..4548)
                     /gene="radC"
                     /locus_tag="VD791_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HXV08772.1"
                     /translation="MGIADWPAGERPRERLLARGAGALTDAELVALCLRTGVRGMSAV
                     DLGRELLARHGGLAGLLGAGHRELARIKGLGTAKSALLAAVLELARRALQEQLRAGTA
                     LTAPGAVRDYLRLALGSRPHEAFLCLFLDAQHRVSGIEELFRGTLTQTSVYPREVVKA
                     ALAANAAAVIFAHNHPSGVAQPSQADELLTRQLKEALALVDIRVLDHFIVAGNQALSF
                     AERGLL"
     gene            4610..>5214
                     /gene="coaBC"
                     /locus_tag="VD791_12130"
     CDS             4610..>5214
                     /gene="coaBC"
                     /locus_tag="VD791_12130"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HXV08773.1"
                     /translation="MTLKGKKLLLGITGGVAAYKAAQLARLLVKEGADVRVAMTEAAT
                     RFVTPVTMQALTGQTVWTDLWDPRVRDNMGHIELSRDRELIVVAPATADFLAKLAGGL
                     ADDLLSTLCVARRCPLLAAPAMNVEMWENAATQRNVHTLRGDGVHLAGPAAGDQACGE
                     VGMGRMLEPEEILAEIEALFQPKQLAGKRVLVTAGPTEEPI"
BASE COUNT          766 a         1792 c         1844 g          812 t
ORIGIN      
        1 tgtatccgcg cgcgattcgc tggtttctcg acggattgct ggagctggag aatgggaagg
       61 tccgggtgaa aggcggccat gcgcagatgg tgcacagcgc tcgcgtctag cctcctggcg
      121 gcgggctgcg cggcgcatgc cgcgccgccg gggcgcgtgg tcatcgacta cgaggtgcgc
      181 tacaacggca gcgccatggc cacggcgcag cacgtgctcg agcacgacgg aaagacctat
      241 cggctcctcg agacctggga gggcagcggc ctgctgtcgc tgttgggaga ggtccggcgc
      301 acgagcgagg gcttggtgac gccggaaggc ctgcgcccgc tggtctacga agaccgccgg
      361 ccgcgccgtg accttgcgat ggcgcggttc tcgtggaagg agcgcaccct cacccagcag
      421 ttccggggcg cgccgcgcac cgagccgatg ccgccgaacg cccaggaccg cctgagcctc
      481 gtgttcgccc ccgccttcaa ggtgcccggc aagggtccgc tggattttca cgtcgcggac
      541 ggcaagggcg tggcgcacta cgtgttcgag atcgcgggcc gcgagcggct gcgcgtgccg
      601 gccggcgagt tcgacgcgct gcatctcgtc aagcgcgaca acgacgggcg agcgacggag
      661 tggtggctgg acgccggcca cgcttacctg ccattgcgcg tgctcgtggt ccgcaaggat
      721 ggtacgcgca tcgaccagct ggcggcgcgc atcacgcccg cgccgtgatc ttccgcgcac
      781 agctggcgag agcagaggcg ctgctcgggg agctgctcgc gcagggcggg ccggcggacg
      841 cggcggtgtc gcgctacttc cgggcgcacc gcaacctcgg ccagcaggat cgcgccttcg
      901 tcgccgagac ggtgtacgcg gtgctgcgcc ggcggcgctc gcttgccgcg gcggcgggct
      961 cggatgcgcc gcgcgccctg gcgatcgccg cgctcgtgcg cgtgcgcggc ctgtcgggac
     1021 gcgcgctcga aggcgtgctg cgggaggacg agcaggcgct ggtggcgcgc gtgcgtgccg
     1081 cgcgcagcga gacgtttgcc cctgcggtgc gcgccgatct ccccgactgg ctgtgggagc
     1141 gcctggcggc gctgcatggc gaagacgagg ccatgcgcat cgcccagggc ctgctcaatc
     1201 cggcgccgct cgacctgcgg gtcaatctcg ccaaggcggg cagggacgag gcgctcgcgc
     1261 ggctgcgcgc cgatggcatc gaggcagcgc cgaccccgca ctcgcccgcc ggcatccggc
     1321 tcgcgggcaa gccggccgtc aatcgccacc cgttgttcca ggggggcgtc atcgaagtgc
     1381 aggatgaggg cagccagctg ctcgcctatc tccttgcgcc gcgacgcggg gagatgatcg
     1441 ccgactattg cgcgggcgcg gggggcaaga cgctcgcgct ggccatgctc atgcggggca
     1501 ctggccgcat ctacgccatg gatgtgtcca gccggcgcct cggcgagctt gcgccgcgcg
     1561 cggcgcgcgc tggcgtttcc aacgtgcacg cgctggtgct ctccggcgag ggtgacgcgc
     1621 gcgccaagcg gctggcgctg aagctcgacc gcgtcctggt ggacgcacct tgcagcggct
     1681 ttggcacgct gcggcgcaac cccgacctca aatggcggca tgacgccacg gcgatccgcg
     1741 agctggccgc caagcaggcg gcgatccttg ccacggccgc gcgcctggtg aagcccggcg
     1801 gccggctggt gtacgcgacc tgcagtatcc tgcccgagga gaacgaagac gtggcgcgcg
     1861 cctttgccgc tgcgcacccg gagttccagc cgctctcgtg ccgggagatc ctggcggcgc
     1921 agcgcatcgc gctggattgc gacgagcaac tgcggctctg gccgcatcgc cacggctgcg
     1981 acggcttctt cgccgccgcg ttcgagcgcc gttgagcgac tttccccttg cggtacgggc
     2041 ggttacggcg gaagcgggaa ttcctcacca cattagggga attcccggtt gcgcgcaggg
     2101 tccaatcgcg tagaatcgtg cgccactcct tacataacta ttagaatcta cagatgattt
     2161 tctctggttt gttcgacctg ccctggtggg gctacgtgct ggtggccctc ggcctgacgc
     2221 acgtcaccat cgcgtcggtc accatcttcc tgcaccggca ccaggcgcac cgcgcgctgg
     2281 acctgcatcc gatcgcgtcg catttcttcc gcttctggct ctggctgacc accggcatgg
     2341 tgaccaagga gtgggcggcc atccaccgca agcaccacgc caagtgcgag acgcccgaag
     2401 acccgcacag cccgcaggtc ctgggcatca atcgcgtcct gtggggcggc gtgttcctgt
     2461 acgtgaagga gtcgtacaag gccgagacga tggagcgcta cggccatggc acgccggacg
     2521 actggatgga gcgcaacctg tacacgcgcc atgcgattct cggtctcacg ctgatgggcg
     2581 cagtcaacct gctgctgttc ggcctgatcc ccggcgcgtt gatcctcatc acgcagatcg
     2641 tctggatccc gttctgggcc gcgggcgtga tcaacggcat cggccactac tggggctacc
     2701 gcagcttcga catcgccgac gccagcaaga acatcgtgcc ctggggcatc ctgatcggcg
     2761 gcgaggagct gcacaacaat caccacgcct acgccacctc ggccaagctg tcggccaagt
     2821 ggtacgagtt cgacatcggc tggatgtaca tccgcctgct cgaggctctc ggcctcgcga
     2881 cggtgaagaa ggtggcgccc acgccgcgct tcgagccggc gaagaccatg tgcgactacg
     2941 acacgctgca ggccgtgatc gccaaccgct acgacgtgct ggcgcgctac gccaaggcga
     3001 tcaagcgcac ctggagcgac gagatcgagc ggctgcagca ctggtcgccg cgcgacgcgg
     3061 agatgctcaa gccgctcaag cgcgcgctgg tgcgcatcgg ctacggcgcg gaggccgatc
     3121 gcgcgcgggt ggccgaggcg ctcaagccct cgaagccgtt gcaggtggcg gtgagcatgc
     3181 gcgaggagct gatcgcgctg tgggagcgct cgcacgcgtc caaggagcag ctgctgcgcc
     3241 agttgcagga ctggtgccag cgcgccgaag cgagcggcat cgcaccgctg atcgacttct
     3301 cgcggcgcct gcgcagctac gcctgagcgc ccgcagcaac gaaaaaggcc cgcgcgagcg
     3361 ggccttttgt tttgtgccgg gccgtccggc tacttgagct tggcttcctt gtagaggacg
     3421 tgcttgcgcg ccttgggatc gtacttcttg atctccatct tgtccggcgt ggtgcgcttg
     3481 ttcttggtcg tggtgtagaa gtggccggtg ccggcggtcg actcgagctt gatcttgtcg
     3541 cgcatcagcg ggactccttc tgcttggctg ccgccttgtc ccgcgcgcgg atctccgcca
     3601 gcacggcttc gacgcccagc ttgtcgatct tgcgcagccc cgcggtgctg acccgcaggc
     3661 gcacccagcg gttctggctc tccacccaga agcggcggta atgcaggttg ggctggaaca
     3721 ggcgccgggt cttgttgttg gcgtgcgaga ccaggttacc tttcatcggc ttcttgccgg
     3781 tgacttggca gacgcgggac atggcggact ccgaaatgcg gaaaggcgcg gattatagag
     3841 aaaaatccgg gtcctgtccc cggttttctc ccgctacagc agcccccgct cggcaaacga
     3901 aagggcctgg ttgccggcga cgatgaaatg gtcgagcacc cggatgtcca ccagcgccag
     3961 cgcctccttg agctgccgcg tcagcagctc gtccgcctgc gacggctgcg ccacgcccga
     4021 ggggtggttg tgcgcgaaga tcacggccgc agcgttggcc gccaacgccg ccttcacgac
     4081 ctcgcgcgga tacacgctgg tctgcgtcaa cgtgccgcgg aacagctcct cgatgccgga
     4141 gacgcggtgc tgcgcatcga ggaacaggca tagaaacgcc tcgtgaggcc ggctgccgag
     4201 ggcgaggcgc aggtagtcgc gcaccgcccc cggcgcggtg agcgcggtgc cggcgcgcag
     4261 ctgctcctgc agcgcgcggc gggcgagctc gaggaccgcc gccagcagcg ccgacttggc
     4321 cgtgcccagg cccttgatgc gcgcgagctc gcggtgtccg gcgcccagca gccctgcgag
     4381 gcctccatgg cgcgcgagca gctcgcgccc caggtcgacc gcgctcatgc cgcgcacgcc
     4441 cgtgcgcagg cacagggcga cgagctcggc gtccgtcagc gcgccggcgc cccgggcgag
     4501 caggcgctcg cgcgggcgct ctcccgcggg ccagtcggcg atgcccaaag cgataccccc
     4561 cgatttcccc tagaatctcc tttctcttaa cgcgccagcc cgacgcacga tgaccctgaa
     4621 gggcaagaag ctgctgctgg gcatcaccgg cggggtcgcg gcctacaagg ccgcgcagct
     4681 cgcccgcctc cttgtcaagg agggcgcgga cgtgcgcgtc gccatgaccg aggccgccac
     4741 gcggttcgtg acccccgtga cgatgcaggc cctcaccggt cagaccgtct ggacggatct
     4801 gtgggacccg cgcgtgcgcg acaacatggg tcacatcgag ctgtcgcgcg accgggagct
     4861 gatcgtggtc gcgccggcca cggccgattt cctggccaag ctcgccggcg ggctggccga
     4921 tgacctgctg tccacgctgt gcgtggcgcg ccgctgcccg ctgctggcgg cgccggcgat
     4981 gaacgtggaa atgtgggaga acgccgccac ccagcgcaat gtgcacacgc tgcgcggtga
     5041 cggcgtgcac ctcgccgggc cggccgcggg cgaccaggcc tgcggcgagg tcggcatggg
     5101 ccgcatgctc gagccggagg agatcctcgc cgagatcgag gcgctgttcc agcccaagca
     5161 gctggccggc aagcgcgtgc tggtgaccgc gggccccacc gaggagccca tcga
//
LOCUS       DASPGE010000376         6979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1503024, whole genome shotgun sequence.
ACCESSION   DASPGE010000376 DASPGE010000000
VERSION     DASPGE010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503024"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..169
                     /locus_tag="VD791_12135"
     CDS             17..169
                     /locus_tag="VD791_12135"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HXV08774.1"
                     /translation="MFSVDAGTAGHPGLYVLDAAAVPRALGVNPLLAITALAERSAEH
                     LLRDLG"
     gene            complement(182..826)
                     /locus_tag="VD791_12140"
     CDS             complement(182..826)
                     /locus_tag="VD791_12140"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXV08775.1"
                     /translation="MREDEERLAYAFALSGGFAILLWLIKIFESVTGFDTIPYGVYPH
                     KLSGLLGIFTAPLIHGSPEHLLANTLPIVVLGTALLYGYPRSARIVLPVLFLGSGLGV
                     WLIGRESYHVGASGLSFGMMFFVFTQGVLRWDRRAIGLAMVVFFLYGGMVWGVFPGDP
                     QVSYESHLSGAILGVALAFLLRRRDPPPPVKRYSWEDEAPEQPPVDQPPSESRQ"
     gene            complement(831..1274)
                     /locus_tag="VD791_12145"
     CDS             complement(831..1274)
                     /locus_tag="VD791_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08776.1"
                     /translation="MPDRLPLGSSRARRALMLGISLLALSDARADSGSIARCIHDVGE
                     FGNEMVQMCAAEDVAAEKALSQYPEKTKQIVAGCSRNVEQTGWVVAKRCADRNIAAAD
                     ALADYPQAHQPLIRTCTRKVGDLGQVHVKACVDQEIAAGKAPRQD"
     gene            complement(1274..2095)
                     /locus_tag="VD791_12150"
     CDS             complement(1274..2095)
                     /locus_tag="VD791_12150"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV08777.1"
                     /translation="MTRSLPTLLLALATAGAPAAAERLPVFDAHVHYNFDFGRPLPVQ
                     AVLDTWRDAGIRGVLLNSRPNDGTRELLAAAPPEFKAVAFARPYVVMSDVQTWFRDPA
                     IYDMIERELERGIYVGLGEFHIYGRDADSEGFARFMRLAQQRGLWLHAHCDDYVIERI
                     FALYPGARVIWAHTGMGIAEGRIDELFARYPALHGELSYRGGIAESGTLSPAWRQLFT
                     KYPQRFVLGSDTWMPQRWPDVPAIMDGYRAWLSQLPREVAENIAWRNGARLFFGE"
     gene            complement(2178..3110)
                     /locus_tag="VD791_12155"
     CDS             complement(2178..3110)
                     /locus_tag="VD791_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXV08778.1"
                     /translation="MASKSSPLPASIDDTLALLSRGDYVAERSLATAVFLALRMGRPL
                     FLEGEAGVGKTEIAKVLAHTLGRRLIRLQCYEGLDIASAVYEWNYPRQMIEIRLAEAA
                     GAASREALGQDLFSERFLIRRPLLQALEPDTVPPVLLIDELDRTDEPFEAYLLEVLSD
                     FQITIPEIGTIKARQPPIVVMTSNRTREIHDAVKRRCLYHWVDYPSAERELEILARKV
                     PGAPQRLSQEVVAFVQKLRGMDLFKAPGVAETIDWSRALVELDRIALDPATIDNTLGA
                     LLKYQDDIARIQGSEAARLLAELRAGSGRTPPSP"
     gene            complement(3149..3949)
                     /locus_tag="VD791_12160"
     CDS             complement(3149..3949)
                     /locus_tag="VD791_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXV08779.1"
                     /translation="MYTFNYHKAGSVNDAASRLAGNPDAKLLAGGQTLIASMKLRLTS
                     PPDLVDLRDVAELRGIKADGNAITVGAMTRHAEVAESKEVQRAIPALARLAAGIGDRM
                     VRNMGSLGGSIANADPAADYPAAVVGLNASVSTSKRKIAADDFFTGMFETALQPGEII
                     TSVAFPVAKRTAYMKFKNPASRYAIVGVFVADTGGGVRVAVTGAGPSVFRVAEMERAL
                     SSKFVPDALNGIKVSASGLNSDLHASAEYRAHLITVMAKRAVEQALAG"
     gene            complement(3984..6341)
                     /locus_tag="VD791_12165"
     CDS             complement(3984..6341)
                     /locus_tag="VD791_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXV08780.1"
                     /translation="MGAMNFIGEAVKRREDYRFLTGAGQYTDDVQVNGQTYAVFVRSP
                     HAHAKIRRIDTSKARKAPGVVGIFTGEDVANAKIAGLPCGWLITDVNGQPMKEPPHPV
                     LAQGKVRYVGDHVAVVIAETQLQARDAAELVEVDYEVLPAVVDVSAARKPGAPAIHDI
                     APDNTCYVWALGDKGAVDKAFASAHQVTKLEFVNNRLIPNAMEPRSALGSYSRADDGY
                     TLYVASQNPHVERLLMTAFVLGLPEHKVRVIAPDVGGGFGSKIYLYAEDVVVTWASKQ
                     VNRPVKWTAERSESFTSDAHGRDHVTVAELALDKDGKFLAMRVRTTAAMGAYLSTFAS
                     CIPTILYATLLAGQYTTPVIYCEVTAVFTNTAPVDAYRGAGRPEATYVVERLVETAAR
                     DMKIDPAELRRRNFITQFPYQTPVALLYDTGNYRKTLDSAMKLADVAGFPARKAEAAK
                     RGKLRGLGYSCYIEACGIAPSAVAGSLGARAGLFEAGEVRVHPTGKVTVFTGSHSHGQ
                     GHETTFAQVVASRLGIPMDDVDIVHGDTGRILFGMGTYGSRSLAVGATAIVKAVDKVI
                     AKGKKIAAHLMEASEADVEFSDGAFKVAGTDKQVPFAQVALTAYVPHNFPHDKLEPGL
                     NENAFYDPTNFTFPAGSHLCEVEVDPDTGTTKIVSFTAVDDFGNVVNPMIVEGQVHGG
                     LVQGIGQALLEGCRYDKESGQLLTGTLLDYCVPRADDVPFFQVGTEVTPCTHNPLGVK
                     GCGEAGAIGAPAAVMNAITDALGVRDLEMPATPERVWRSARKLAA"
     gene            complement(6346..6816)
                     /locus_tag="VD791_12170"
     CDS             complement(6346..6816)
                     /locus_tag="VD791_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXV08781.1"
                     /translation="MPVRLSMTVNGRPVSAEVETRTLLVNFIREQLRLTGTHVGCDTA
                     QCGACTIHMNGKAVKCCNMLAIQAEGAQIVTIEGVAAADGKLHPMQEAFREHHGLQCG
                     FCTPGMVMSAIDLVRNHPNPSEETVRAELDGNLCRCTGYHNIVKAVQAGAKAMR"
BASE COUNT         1051 a         2404 c         2381 g         1143 t
ORIGIN      
        1 aaatcagaag ggccaggtgt tcagcgtcga cgcgggcacg gccgggcacc ccggcctgta
       61 cgtgctggat gctgccgcgg tgccgcgcgc gctcggcgtc aatccgctgc tggcgatcac
      121 ggcgctagcg gagcgcagcg ccgagcatct gctccgcgac ctcggctgag cgttcgcgcg
      181 ctcactgcct cgattcgctc ggcggctgat cgaccggggg ttgttcgggg gcctcgtctt
      241 cccagctgta tcgcttgacc ggcggcggcg gatcgcgccg gcgcagcagg aacgccagcg
      301 caacgccgag gatcgcaccg gacaggtggg actcgtagga gacctgcgga tcgccgggaa
      361 acacgcccca caccatcccg ccgtagagga agaacaccac catggcgagc ccgatggcac
      421 ggcgatccca gcgcagcacg ccctgggtga agacgaagaa catcatgccg aagctcaatc
      481 cgctcgcgcc cacgtgatag gactcgcggc cgatcaacca gacgccgagc ccgctgccga
      541 ggaagagcac ggggagcacg atgcgcgccg acctggggta cccgtacagc agcgccgtgc
      601 cgaggacgac gatcggcagc gtgttggcga gcagatgctc gggcgagccg tggatcaacg
      661 gcgccgtgaa aatccccagg agaccgctga gcttgtgggg atagacgccg tacgggatcg
      721 tgtcgaagcc ggtgacggac tcgaagatct tgatcagcca caggaggatg gcgaagccac
      781 ccgacagcgc gaaggcgtag gcgagacgct cctcgtcctc cctcacctgg tcaatcctgc
      841 cgcggcgctt tcccggctgc gatctcctgg tccacgcacg ccttgacgtg cacctgaccg
      901 aggtcgccca cctttcgggt gcacgtccgg atcagcggct ggtgcgcctg cggataatcc
      961 gccagcgcat ccgccgctgc gatgttccga tcggcgcagc gcttcgcgac cacccagccc
     1021 gtctgctcga cgttgcgtga gcaaccggcc acgatctgct tggtcttctc gggatactgc
     1081 gacagggcct tctccgccgc aacgtcctcg gccgcgcaca tctggaccat ctcgttgccg
     1141 aactcgccca cgtcgtggat gcagcgcgcg atgctgcccg aatcggcccg agcgtccgac
     1201 aaggcaagca gcgaaatgcc gagcatcagc gcgcgacgcg cccgtgacga tcccaggggc
     1261 aggcggtcag gcatcattca ccgaagaaca gccgtgcgcc gttgcgccag gcgatgttct
     1321 ccgcgacctc gcgcggcagc tgcgacagcc aggcccggta gccgtccatg atcgcgggca
     1381 cgtccggcca acgctgcggc atccaggtgt cggagccgag cacgaagcgc tgcggatact
     1441 tggtgaagag ctgccgccag gcgggggaca gcgtgccgct ctcggcgatg ccgccgcggt
     1501 aggacagctc cccgtgcagc gccgggtagc gcgcgaacag ctcgtcgatg cgcccttccg
     1561 ctatgcccat gccggtgtgc gcccagatca ccctggcgcc ggggtagagc gcgaagatgc
     1621 gctcgatcac gtagtcgtcg cagtgggcgt gcagccacag gccgcgctgc tgcgccaggc
     1681 gcatgaagcg ggcgaagcct tcgctgtcgg cgtcgcgacc gtagatgtgg aactcgccca
     1741 ggccgacgta gatgccgcgc tccagctcgc gttcgatcat gtcgtagatc gccgggtcgc
     1801 ggaaccaggt ctgcacgtcg ctcatcacga cgtacgggcg cgcgaaggcc accgccttga
     1861 actccggcgg cgcggcggcc agcagctcgc gcgtgccgtc gttcggccgg ctgttgagca
     1921 gcacgccgcg gatgccggca tcacgccagg tgtcgagcac ggcttgcacc ggcagcgggc
     1981 ggccgaaatc gaagttgtag tgcacgtggg cgtcgaacac cggcaggcgt tcggcggccg
     2041 cgggcgcgcc ggccgtcgcc agcgccaaca gcaaggtcgg tagcgatcgt gtcatcgcct
     2101 ggaaggcgca tttcggcgcc aacaaagcac cgctaagctc gaaaaacacc cgcatgtgag
     2161 tcgatattct cggcacttca aggacttggc ggggtccgac ccgaacccgc gcgaagctcg
     2221 gccagcaggc gggcggcttc gctgccttgg atgcgggcga tgtcgtcctg gtacttgagc
     2281 agcgcaccga gcgtgttgtc gatggtggcc gggtcgagcg cgatgcggtc cagctccacc
     2341 agcgcgcgcg accagtcgat ggtttcggcc acgcccggcg ccttgaacag atccatgccg
     2401 cgcagcttct gcacgaacgc caccacctcc tgcgacaggc gctgcggcgc acccggcacc
     2461 ttgcgcgcga ggatttccag ctcgcgctcc gccgacggat agtcgaccca gtggtacagg
     2521 cagcgccgct tcaccgcgtc gtggatctcg cgcgtgcggt tggaggtcat caccacgatc
     2581 ggcggctgcc gcgccttgat cgtgccgatc tcggggatgg tgatctggaa gtcggacagc
     2641 acctccagca gataggcctc gaacggctcg tcggtgcgat ccagctcgtc gatcaggagc
     2701 accggcggca cggtgtccgg ttccagcgcc tgcagcagcg ggcgacggat caggaagcgc
     2761 tcggagaaca ggtcctgccc cagcgcttcg cgcgaggccg cgcctgcggc ctcggcgagc
     2821 cggatctcga tcatctggcg cgggtagttc cactcgtaca ccgccgaggc gatgtccagc
     2881 ccctcgtagc actgcaggcg gatcaggcgc cggccgagcg tgtgcgccag gaccttggcg
     2941 atctcggtct tgcccacgcc cgcctcgccc tccaggaaca gcggccggcc catgcgcagc
     3001 gccaggaaca ccgccgtggc gaggctgcgc tcggcgacgt agtcgccgcg cgagagcagg
     3061 gcaagcgtgt cgtcgatgga ggcggggagg ggtgaggatt tggatgccat ggaatggcag
     3121 gatacctcag tccctcccct cggcagaact agccggcgag cgcctgctcc accgcgcgct
     3181 tcgccatcac cgtgatcaga tgcgcgcggt actcggcgct ggcgtgcagg tcggagttca
     3241 ggccgctcgc cgacaccttg atgccgttca gcgcgtccgg cacgaacttg ctcgacaggg
     3301 cccgctccat ctccgccacg cggaacacac tcggtccggc gccggtgacc gccacgcgca
     3361 cgccgccgcc ggtgtcggcc acgaacacgc ccacgatcgc gtagcgcgac gccgggttct
     3421 tgaacttcat gtacgcggtg cgcttcgcca ccgggaaggc gaccgaggtg atgatctcgc
     3481 ccggctgcag cgcggtctcg aacatgccgg tgaagaagtc gtcggccgcg atcttgcgct
     3541 tgctggtgct gacgctcgcg ttcaaaccca ccaccgcggc gggatagtcc gccgccggat
     3601 cggcgttggc gatggagccg cccaggctgc ccatgttgcg caccatgcga tcgccgatgc
     3661 cggcggcgag cctggccagc gccgggatcg cccgctgcac ctccttcgat tccgcgacct
     3721 ccgcgtgccg cgtcatcgca ccgacggtga tggcattacc gtccgccttg atcccgcgca
     3781 gctcggccac gtcgcgcaga tctacgaggt cgggcggcga ggtgaggcgc aacttcatgg
     3841 atgcgatcag ggtctggccc cccgcgagca gcttggcgtc ggggttgccc gccagccgcg
     3901 aggcggcgtc gttcacgctg ccggccttgt ggtaattgaa tgtgtacatg ctgtcctcca
     3961 gtgttctcga gccgctgctg ttctcaagcc gccaacttgc gcgcggagcg ccacacccgc
     4021 tcgggcgtgg ccggcatctc caggtcgcgc acgccgagcg cgtcggtgat cgcgttcatc
     4081 accgccgccg gcgcgccgat cgcaccggcc tcgccgcagc ccttcacgcc cagcgggttg
     4141 tgggtgcagg gcgtgacctc ggtgccgacc tggaagaacg gcacgtcgtc cgcgcgcggc
     4201 acgcagtagt cgagcagggt gccggtgagc agctgcccgc tctccttgtc gtagcgacag
     4261 ccctccagca gcgcctggcc gatgccctgc accaggccgc cgtgcacctg gccctcgacg
     4321 atcatcgggt tcaccacgtt gccgaagtcg tccactgcgg tgaagctcac gatcttcgtg
     4381 gtgccggtgt ccggatcgac ctccacctcg cacaggtggc tgccggccgg gaaggtgaag
     4441 ttggtcgggt cgtagaacgc gttctcgttg agccccggct cgagcttgtc gtgggggaag
     4501 ttgtgcggca cgtaagcggt gagcgcgacc tgggcgaacg gcacctgctt gtcggtgccg
     4561 gccaccttga acgccccgtc gctgaactcg acgtcggcct cggaggcctc catcaggtgc
     4621 gcggcgatct tcttgccctt ggcgatcacc ttgtccaccg ccttgacgat ggcggtggcg
     4681 cccaccgcca gcgagcgcga gccgtacgtg cccatgccga acaggatgcg gccggtgtcg
     4741 ccgtgcacga tgtccacgtc gtccatcggg atgcccagcc ggctggcgac cacttgcgcg
     4801 aacgtggtct cgtggccctg gccgtggctg tgcgagccgg tgaacaccgt caccttgccg
     4861 gtgggatgca cgcgcacctc gccggcctcg aacaggcccg cgcgcgcacc gagcgaaccg
     4921 gccaccgccg agggcgcgat gccgcaggcc tcgatgtagc aggaatagcc caggccgcgc
     4981 agcttgccgc gcttggccgc ttccgccttg cgcgcgggga agccggccac gtcggcgagc
     5041 ttcatcgcgc tgtccagcgt cttgcggtag ttgccggtgt cgtacagcag ggcgaccggc
     5101 gtctggtagg gaaactgggt gatgaagttg cgccggcgaa gctcggccgg gtcgatcttc
     5161 atgtcgcgcg cggcggtctc gaccaggcgc tccaccacgt aggtggcttc cggccggccg
     5221 gcgccgcggt acgcgtccac cggcgcggtg ttggtgaaca ccgccgtgac ctcgcagtag
     5281 atcaccggtg tggtgtactg gccggcgagc agcgtcgcgt acaggatggt cgggatgcag
     5341 gatgcgaacg tggacaggta cgcgcccatc gccgccgtgg tgcgcacgcg catcgccagg
     5401 aacttgccgt ccttgtccag cgccagctcg gccaccgtca cgtggtcgcg gccgtgcgcg
     5461 tccgaggtga aggactcgct gcgctccgcg gtccacttca ccgggcggtt cacctgcttg
     5521 gaggcccagg tgaccaccac gtcctcggcg tacaggtaga tcttggagcc gaagccgccg
     5581 cccacatcgg gcgcgatcac gcgcaccttg tgctcgggca ggcccagcac gaaggcggtc
     5641 atcagcaggc gctcgacgtg cgggttctgg ctggcgacgt acagcgtgta gccgtcgtcc
     5701 gcgcggctgt agctgccgag cgccgagcgc ggctccatgg cgttggggat caggcggttg
     5761 ttgacgaact cgagcttggt gacctggtgc gccgaggcga acgccttgtc caccgcaccc
     5821 ttgtcgccga gggcccagac gtaacaggtg ttgtccggcg cgatgtcgtg gattgccgga
     5881 gcgccgggct tgcgcgcggc ggaaacgtcc accaccgctg gcagcacctc gtagtcgacc
     5941 tccaccagct cggccgcgtc gcgtgcctgc agctgggtct cggcgatgac caccgccacg
     6001 tggtcgccca cgtagcgcac cttgccctgc gccagcaccg gatgcggcgg ttctttcatg
     6061 ggctggccat tgacgtccgt gatcagccag ccgcagggca gcccggcgat cttggcgttg
     6121 gcgacgtcct cgccggtgaa tatgcccacc acccccggcg ccttgcgcgc cttgctggtg
     6181 tcgatgcgac ggatcttcgc gtgcgcgtgg ggggagcgca cgaacaccgc gtacgtctgg
     6241 ccgttcacct gcacgtcgtc cgtgtactgg ccggcccccg tgaggaagcg gtaatcctcc
     6301 ctgcgcttga ccgcttcgcc gatgaagttc atcgcgccca tggtgtcacc tcattgcctt
     6361 cgccccggcc tgcacggcct tgacgatgtt gtgatagccg gtgcagcggc acaggttgcc
     6421 gtcgagctcg gcacgcaccg tctcctcgga cgggttcgga tggttgcgca ccaggtcgat
     6481 ggcgctcatc accatgcccg gcgtgcagaa gccgcattgc agcccgtggt gctcgcggaa
     6541 cgcctcctgc atcgggtgca gcttgccgtc ggcggcggcg acgccctcga tggtgacgat
     6601 ctgcgcgccc tcggcctgga tcgccagcat gttgcagcac ttcaccgcct tgccgttcat
     6661 gtggatggtg caggccccgc actgggcggt gtcgcagccg acgtgcgtgc cggtgaggcg
     6721 cagctgctcg cggatgaaat tcaccagcag cgtgcgcgtc tcgacttccg ccgagaccgg
     6781 ccgtccgttg acggtcatgg atagcctgac tggcatggct cctcctcctt ctaatgatca
     6841 agttcgattg ggctgtgagg acggggttcg ccaagcgcga ttcagcgctc cgccttgcaa
     6901 ttgcgcggcg tgatgttgtt ctccttggcg tacttggcgg ccgactcctc gaccatgggc
     6961 cgcacgatgg actggtccg
//
LOCUS       DASPGE010000377        21071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1738564, whole genome shotgun sequence.
ACCESSION   DASPGE010000377 DASPGE010000000
VERSION     DASPGE010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21071
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738564"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VD791_12175"
     CDS             complement(<1..1028)
                     /locus_tag="VD791_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08782.1"
                     /translation="MQGLGRKLVAISLFGILAVPAGASEEDIAFLAARDAFQKGRIEK
                     LDSLAAGLADHPLYPYVAYWQLRSRLNQTPEAEVEAFLTRYGDALAAQRLRTDWLKQL
                     GERQEWSTFEHEIRGVVLDDPELVCYLLRARLARVDLTALPEARALWFQGVAQPQSCA
                     PLFERMMAGGQITEEDIWARIRLALEAGSITLAKSLGGHIDPAKRIAGKQLDAVARNA
                     QRYLERRPLPLRTRAERELAMFATWRVAQSLPAVAATRLEKFENALSTEERAYVWGQV
                     AMAGAQRHRPEALDWFKRAAETGLSDKQLAWRTRTGLRLADWATVLSSIEAMSPAEQQ
                     HVVWRYWKS"
     gene            complement(1094..2617)
                     /gene="ilvA"
                     /locus_tag="VD791_12180"
     CDS             complement(1094..2617)
                     /gene="ilvA"
                     /locus_tag="VD791_12180"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HXV08783.1"
                     /translation="MPDYLERILKARVYDVAQETPLEPAANLSARLGNRLLLKREDVQ
                     PVFSFKLRGAYNRMVHLPAPVLRRGVICASAGNHAQGVAFAAQKLGCRAVIVMPATTP
                     RIKLEAVAARGARVVLHGDSYDDAYAHSTVLARRERLTYVHPYDDPDVIAGQGTIGME
                     ILREHSAPIHALFAAVGGGGLIGGVAAYVKRLRPEIRILGVQPSDSDAMARSLAAGRR
                     VRLPQVGLFADGVAVKEPGAETFRLCRELVDEMVLVDNDDICAAIKDVFEDTRTILEP
                     AGALAIAGAKAWVERRRIRDKTLIAIACGANMNFDRLRFVAERAELGERREAILAVTI
                     PERPGSFKRFCALLGPRNITEFNYRYGDPERARVFVGLSTHNREETAQLVRRLRHAGL
                     PTADLSDNEMAKLHIRHMVGGRSSQVSDEMVYRFEFPERPGALMNFLNRMSQGWNISL
                     FHYRNHGADFGRVLAGMQVPGDQQRAFKRFLTQLNYRYWDETGNPAYELFLGMRAGA"
     gene            2715..3374
                     /gene="rpiA"
                     /locus_tag="VD791_12185"
     CDS             2715..3374
                     /gene="rpiA"
                     /locus_tag="VD791_12185"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HXV08784.1"
                     /translation="MTAEQMKRAAAEAAIEHVPPGAVVGVGTGSTVNFFIEALGRIKG
                     RIEGAVASSEASAERLRRLGIPLLELNSVERLPIYVDGADEVTRRLHMIKGGGGALTG
                     EKIVAAVARQFVCIADEGKLKEVLGAFALPIEVIPLARAHVAREMARLGGQARLREGF
                     VSDYGNLILDVHGLSIRDPVEMETAIDGIAGVVTNGLFARRRADLLLLGTPLGVRSIG
                     A"
     gene            3498..4208
                     /gene="phoU"
                     /locus_tag="VD791_12190"
     CDS             3498..4208
                     /gene="phoU"
                     /locus_tag="VD791_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HXV08785.1"
                     /translation="MNTEHTHTSKQFDAELENLRSRVLQMGGFVEQQITLAVDALTSG
                     DLELCASVVGNDHKVNAMEVGIDEDCSTIIARRQPTASDLRLVIAVIKTITDLERIGD
                     EAAKIARMAKLINSVDRVQQPRYVEIRRMASLALEMLRKSLDCFARLDVSGSAEVVRT
                     DQHVDDEFRAILRQLITFMMEDPRTISASIEILFIAKAIERIGDHAKNISEYVIYMVK
                     GKDVRHVTLEEIEREIMA"
     gene            4205..5728
                     /gene="ppx"
                     /locus_tag="VD791_12195"
     CDS             4205..5728
                     /gene="ppx"
                     /locus_tag="VD791_12195"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HXV08786.1"
                     /translation="MTQLSTLAAVDLGSNSFRLQVARVVDDQIYPLDSLREPVRLAAG
                     ITADKRLDEASQQRALDALKRFGERLRGLPPEAVRAVGTNALRMAKNAREFLVKAEAA
                     LGFPIEVVAGKEEARLIYLGVAHSLPVTGERRLVVDIGGGSTECIIGAGYRPQRLESL
                     YMGCVSYSLRFFPGGKLTKGNFKQAETAARLELQTIARDFSAGRWKEAVGSSGTARAL
                     GDILEANGWSRGGIAAEGLERLRSALLKAAELDRLDLPGLRPDRVPVLPGGFAIMAAA
                     FSALRIEHMTLATGAMRQGVLYDLLGRFHDRDMRDSTVAQFMKRYHVDAGQARRVESL
                     AQSLLRQFSGALQAEHGYPLHLLSWAARLHEIGFSVAHSGYHKHSAYILAYADMPGFS
                     RMEQAHLSLLVLAHRGTMEKMKGQISSSLDLAMVMALRLAVLFHRSRSDVRLPPFEAR
                     CEGKRFQLGLDAAWLAASPLTAAALTEEVKEWRKLGVALDIAALEEVEVEGELLPAD"
     gene            complement(5835..6521)
                     /locus_tag="VD791_12200"
     CDS             complement(5835..6521)
                     /locus_tag="VD791_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXV08787.1"
                     /translation="MKPEIKVSAFLANLPLFKEIDSEEIERVAQGTRAVHVERGQVLF
                     HKGDPAEGFYLVVYGQVKLAFTSPQGGDKVVEIIGPGMSFGEAVMFLEKPYPVYAQAL
                     ADSMLLHVSRNAVFQEVERNPQFARKMLGGLSRRLHGLVSDVEAYSLRSGAQRVIGYL
                     LRDDTLAEGGPRAVSITLPTNKGVIASRLNLTPQHFSRILHELTEAGLIRVDGRSVHV
                     PDVNRLRTYE"
     gene            complement(6549..7505)
                     /locus_tag="VD791_12205"
     CDS             complement(6549..7505)
                     /locus_tag="VD791_12205"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HXV08788.1"
                     /translation="MPASAAQTAAQAAAPQRLIARIALAGVLLVLLVTTSSAYLRLTA
                     AGLGCADWPACYGRDLGRNASPMPVARLLHRLSAAAAGAAVFAIALVAFVNRRRLARE
                     RGVATALLALTLGLALLGRATPTAQSPAIATANLLGGMLMAALLWWLALGRWARAQAM
                     VPRWLPWVSALALAALAAQLALGAMVSTTYSALACTSLPDCAGRWWPPTWSVEEFDPT
                     RPLSARTGPSAAIHLAHRYAAAAVAAPVLALATLLRRARRGLAFALAGLLALQVALGA
                     LAVALELPLVLVLAHNFCAVMLLLVLVAAHHASLRPEPPVAE"
     gene            complement(7537..8610)
                     /locus_tag="VD791_12210"
     CDS             complement(7537..8610)
                     /locus_tag="VD791_12210"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HXV08789.1"
                     /translation="MQQTLFSLSARAPQSAAARARRQVAGWLLVCCALVFAMVVVGGV
                     TRLTRSGLSIVEWEPLIGAIPPLSQADWQQLFQEYQQTPEYRQVNRGMSLEQFKGIFW
                     WEYFHRLLGRLIGLVFFLPLVFFALTGRVERALLPRLLGIFLLGGLQGAVGWWMVASG
                     LIDDPRVSHVRLSIHLGLAFLIFAAMFWTALDLLAGGRSDALHPPRARLARYAGWVSA
                     LLFVMVLSGALVAGTRAGYAYNTFPLMNGQFFPPEYLMLQPWWQNFLHNMATVQFNHR
                     LIAWALFLLIPALWFASRRLQLPRSARLASNLLVAMLFAQLGLGIATLLQGVPVVLGA
                     SHQAGALLLFALSLWTAHALRTK"
     gene            8774..8950
                     /locus_tag="VD791_12215"
     CDS             8774..8950
                     /locus_tag="VD791_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08790.1"
                     /translation="MAETVPTPAQDEAEERIPAMQHLLDNHFLLLFLGVVIPAVLYIL
                     WGVMEIVTLPNVPQ"
     gene            8975..9514
                     /locus_tag="VD791_12220"
     CDS             8975..9514
                     /locus_tag="VD791_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HXV08791.1"
                     /translation="MSSILPPPQRIWWKEPIEKIELAWIAIALVWALILFFMMPYWHV
                     FGKQNLSNEAYRISPAQFQAKTQEMVDQYTVRTETEQNVPVVHPPAGSDVYLIARLWS
                     FWPILELEQGKSYRLHLSAMDYQHGFSLQPENVNLQVVPGYEMVITISPNKTGSFAVV
                     CNEYCGIGHHQMVSRIYVK"
     gene            9528..11228
                     /locus_tag="VD791_12225"
     CDS             9528..11228
                     /locus_tag="VD791_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXV08792.1"
                     /translation="MAASFRIDPRSGLQYHDQAEALIKANAVAGVVFLLVGGILSLLV
                     SLTRWPAVHLLDANWFYLALTAHGIDMLIFWIIFFEIAILYFCSSTLLRCRLATPRWA
                     WAGFALMVIGSVMTNIAVFQGESSVMMTSYVPMQAKPAFYLGLILFAVGALIGCFVFF
                     GTLVIARAERTYEGSVPLVTFGAVTAAIIAVFTIASGAIILIPTFLWSIGYISTIDPL
                     MYRVIWWGMGHSSQQINVAAHVAVWYAVGAIVFGAKPMSEKVSRIAFLLYILFLQLAC
                     AHHLLSDPGLSSEWKILNASYFMYFAVLASMVHGLTVPGSIEVAQRRKGFTKGLFEWL
                     RKAPWGNPTFAAMFISLIGFGFLGGISGVMMGTEQLNMLIHNTLYVPGHFHATVVIGT
                     TLAFMGLTYFLLPSLFNREVAFPGVAKWQPYVFGIGMYVLALTLMGAGTLGVPRRHWD
                     IGFAGTTLPYEFPGAAYLMLALVGLSAVLAVLGGGMYILVTVWSILFGKRLEPGSRNT
                     RPSPLRLQPVAAAIEGHGGGIGVLQARGTFTMAMVFLVAFVLYYFINWKYLGSVWGLS
                     "
     gene            11320..12180
                     /gene="cyoE"
                     /locus_tag="VD791_12230"
     CDS             11320..12180
                     /gene="cyoE"
                     /locus_tag="VD791_12230"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HXV08793.1"
                     /translation="MDRFRLVLGLFKLRIGVVIALTAVAGWAVASGPPLAAWQVAVMA
                     VAVLLSSASAGAFNQYVERDIDARMKRTRQRAFVTGALPHSRAWLWVILLLLVGSVAA
                     AAWATNAMSALFVFLGAFFYAVVYTVWLKRRTWLNIVIGGLAGSFAVLAGAAAVDPAI
                     GTVPMLLAVVLFLWTPPHFWSLAMACRQDYEAAGVPMLPVVMGDARCARAIFAHTLVL
                     VSLSLVLAFLGLGPLYLVAALGGGAWFVRASWRLVRTPGPKAAMINFHASLLQLSLLL
                     LAAIVDGAML"
     gene            12177..13058
                     /locus_tag="VD791_12235"
     CDS             12177..13058
                     /locus_tag="VD791_12235"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HXV08794.1"
                     /translation="MIASVSSRLRAALCAGAAAAMLFPGAARSGVSALPALQPSPGQR
                     AAAPSPPALDPGLALTLSQRALGVQVGEHVLHDTQGRAVPLSSFRGKPLLISFVYTGC
                     FTVCPTTTRTLKRAVESAVGTLGSDSFNVVSIGFNLPFDTPQALQAFAHQQGVTLPNW
                     HFLSPDPATLQALTHELGFSYAPLAGGFDHITQVSVVDQSGRVYRQVYGERFPLPQLV
                     GPLKELITGAPPAEQSLAGLLERVRLLCTYYDADTGRYRYRYSLLLEITGGLLGISTM
                     AAFLLYELRKTRRAAGP"
     gene            13104..14738
                     /locus_tag="VD791_12240"
     CDS             13104..14738
                     /locus_tag="VD791_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HXV08795.1"
                     /translation="MRAPDPDRGSLLRDALQRVEATFDRAFGPRANPWRHLGALGFLL
                     FWIVGVSGVYLYALFDTSVQGAYASVEHLSREQWWYGGIVRSVHRYASDAFVLVLLLH
                     LAREWIHGRHRAFRWFTWVSGVPLLWLALAAGIVGFWLVWDELAQFSAIATAEWLDWL
                     GLFAEPLTRNFLRPDSVDDRFFSLLVFLHIGLPLLLLLGMWVHIKRLAHPDTRPGAAL
                     TWGTLAGLTALSVALPVQSGAPADLALAPHALAIDWFYLAPNAAVYAWSPGALWLVAA
                     GVTVLLVTLPWLPRARPQPVAKVDPANCNGCGRCFVDCPYAAITIEPRIIGTIEPRTI
                     GGVAPRANAILDTRNGGRCEPGLAVVDPALCASCGVCAGSCPSSTPFRSVAELITGID
                     MPQLPIGQLRSRLKSQIARLKGEPKLVVFGCDNAVDVRALQRADTAAMNLLCSGQLPP
                     AFVEYALRQGADGVVVTGCGECDCAFRLGNAWTEQRLRGQREPHLRRTVSPAELRVIW
                     ASAQQRDRVVHAVDAFRAELAARGRAAVSAAGNGYA"
     gene            14731..15234
                     /locus_tag="VD791_12245"
     CDS             14731..15234
                     /locus_tag="VD791_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08796.1"
                     /translation="MRNLVAIVGQALLYAAFAACVGLFSMWPPYRHLEPGQALLKLSF
                     SHAGERVAECRRRTPQELAKLPPNMRAPLDCPRERSPVSVELKLDGQVIARRVVSPSG
                     LARDGAATMYERFPVPAGAHRLEVEVDDSVRAPGPTWEREQDVRLAPGRVLVVDLDPE
                     KGGIVIQ"
     gene            15231..16676
                     /locus_tag="VD791_12250"
     CDS             15231..16676
                     /locus_tag="VD791_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXV08797.1"
                     /translation="MKLLPLPSSVRAGAMRAAHPAAAHPAAYRLAVPADGRRTLAAGW
                     LFLGIGALIASGLFSVLLVLSRTPGLKDLFPVADFFRVALVVHVDLSVLVWFVAFAGV
                     LWSLAGTRQGARLAWAGLGLCAVGALALSLAPFLERAPAIMANYIPVLDGPVFLGGLL
                     VFAAGVGLAVLRAMFATQPVLHVLDGPAAIRLGLVAAAVSTAVALFAFLWSWLALPAG
                     LEPRTYYEVLFWGGGHVLQFTWTLLLLVAWLWLADAIGARLPLSPRVVLVLFGLALLL
                     AFFTPFVYLAFDVTSVEHRRALTWMMRVGGGFAILPVALALVLALAQMRDIAQRRRAP
                     RAALAMSLTLFAAGGLIGFAIDGSNVKVPAHYHGCIVGITLAFMGVAYELLPRLGFRA
                     PVSRLAVWQPVIYGTGQLMHIGGLVWSGGYGVQRKVAGADQVLTGLQQVAGMGFMGLG
                     GLIAIVGGVLFLLVVARAVWPARTPLRGALA"
     gene            16687..16905
                     /locus_tag="VD791_12255"
     CDS             16687..16905
                     /locus_tag="VD791_12255"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HXV08798.1"
                     /translation="MNDREPLDVRWLEPPEPFERIVAALEALPAGETLTVMIHREPHP
                     LYRWLTREGWRYNTRYDAAGYFEIRIGR"
     gene            17060..18952
                     /locus_tag="VD791_12260"
     CDS             17060..18952
                     /locus_tag="VD791_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXV08799.1"
                     /translation="MLIQRAGQALFLQVERGFNAAFGDRLNPFYHLGEISFFLFWVVA
                     ASGLYLYAFFDTGVSAAYASVERITHGQWWLGGVLRSVHRYASDAMVLTMLLHLLRHF
                     CFDRYRGFRWVSWVSGIAVLWLVYVSGINGFMLPWDRLAQFVVTGSAEYLDWLPIFNG
                     ALTRNFIYQTSVNDRLFSLLSFIHIGVPLVLLLLLFVHIQRTPKAATAPPRRIWVPLT
                     STLLVLSLIVPVVGDAPADLSVVPERLAFDWFYLATFPLMYAWSVGAAWMLLGGITLA
                     LVLLPWLPPKRVGREGLRAIVHPGDREILVRPDETILEAGLRAGIPMPFECRNGGCGL
                     CKGALLYGRIEHAPYQKSALSDAERAAGKVLFCCATPLTEIEIEYEEAGAARDFPLKA
                     LEVTVRRMRRVSDAVMIVDLALPPGERMHYRAGQYFNVVLEDGARRAFSFATAADDSD
                     RIEMHVRLIPGGRFTTHVFTAMREGDRLRIEGPLGQFALHDSDRPLILVAGATGFAPV
                     KSMLEYAFRAGLKRRMFLYWGVRRRDDLYMHELPERWEREHANFHFVPVLSEAQTEDD
                     WHGRTGLVHEAILHDFPDLAGFEVYTCGSMKMVEAARPAFLGQGLDEDACFADAFFPP
                     APIQTT"
     gene            19006..20652
                     /locus_tag="VD791_12265"
     CDS             19006..20652
                     /locus_tag="VD791_12265"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HXV08800.1"
                     /translation="MRNGRQEEAAMMGIEVLRDSLANKGTAFTLEERKRLELTGLLPA
                     RAETIDEQFARVLANLRAKPSPIEKFVYLAALRDDNETLFFRALIDHLEELLPLVYTP
                     TVGQACSEWSRIWRRPRGLYVTAREHGRVREVLRRWPARKIAVIVVTDGGRVLGLGDL
                     GANGMGIPIGKLALYTACAGVDPALCLPVMLDVGTDTESVRDDPFYLGLRQPRLVPAH
                     YDALIEEFVAAAQEIFPGVLLQFEDFANVNAFRLLARYRDRICAFNDDIQGTAAMALA
                     GLRAAQHLTGRRLAEERLLFFGAGAANTGIATLAVAAMMQAGLTQAEARARCWFMDSR
                     GLVVEGRAGLAAHKQPFAQVHAPIPDLLAAVEAIRPTALIGASGAAGAFTEPVLAAMA
                     ADNRRPVIFALSNPTSKAECTAEQAYAATRGRAIFASGSPFAPVTVEGRSRATGQANN
                     SFIFPGLGLGVLAAGATRVTDAMFLAAADALAGQVSADDLDAGRVFPPAARMREVAAA
                     VATAVARVAHDRGLATRPQPPDLGAAIRESMYRADYAAAG"
     gene            20721..>21071
                     /locus_tag="VD791_12270"
     CDS             20721..>21071
                     /locus_tag="VD791_12270"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HXV08801.1"
                     /translation="MERLAGEIGERNVWRPAALQAAARYIREQWQALGYAVAAQPYRT
                     HGLTCENLEITIPGLRRPNEIVLAGAHYDSVRASPGADDNASGVAALIEIAALLRGQR
                     PARTLKLVAFVNEEP"
BASE COUNT         3030 a         7194 c         7323 g         3524 t
ORIGIN      
        1 gacttccagt agcgccacac cacatgctgc tgctcggctg ggctcatcgc ctcgatcgac
       61 gagagcaccg tcgcccagtc ggccaggcgc aggccggtgc gcgtccgcca ggcgagctgc
      121 ttgtcggaga gcccggtctc ggccgcgcgc ttgaaccagt ccagcgcctc gggacggtgg
      181 cgctgggcac cggccattgc gacctggccc cagacgtagg cgcgttcctc ggtgctcagc
      241 gcattctcga acttctccag gcgggtggcg gcgaccgccg gcaagctctg cgctacccgc
      301 caggtggcga acatggcgag ctcgcgctcc gcgcgggtcc tcagcggcag cggccggcgc
      361 tcgagatagc gctgcgcgtt gcgcgccacg gcgtcgagct gcttgccggc gatgcgcttc
      421 gccgggtcga tgtggccgcc cagcgatttc gccagggtga tgctgcccgc ctccagcgcc
      481 aggcggatgc gcgcccagat gtcttcttcg gtaatctggc cgcccgccat catgcgctcg
      541 aacagcgggg cgcagctctg gggctgggcc acgccctgga accacaacgc ccgcgcttca
      601 ggcagcgccg tcaggtcgac ccgcgccaga cgcgcccgca gcagatagca aaccagctcg
      661 gggtcgtcca gcaccacgcc ccggatctcg tgctcgaagg tgctccattc ctgacgctcg
      721 ccgagctgct tcagccaatc ggtgcgcagc cgctgcgcgg ccagcgcatc gccgtaccgc
      781 gtcagaaacg cctccacctc ggcctccggc gtctggttca ggcgcgagcg cagctgccag
      841 taggccacgt agggatacag ggggtggtcc gcgaggccgg cggccaggct gtcgagcttc
      901 tcgatgcggc ccttctggaa cgcgtcgcgc gccgccagga aggcgatatc ttcctcggag
      961 gcgccggccg ggacggccag gatgccgaaa agtgaaatcg cgacgagttt ccgccccagc
     1021 ccctgcacgc gcccgctccc agttccgccc cccctggttg gttcgccggg gggcgacagc
     1081 attttcgcac agttcatgcg ccggctcgca tcccgaggaa cagttcgtag gcggggttgc
     1141 cggtctcgtc ccagtagcgg tagttgagct gcgtcaggaa gcgcttgaat gcgcgctgct
     1201 gatcgccggg cacctgcatg ccggccagca cgcgcccgaa atcggcgccg tggttgcgat
     1261 agtgaaacaa gctgatattc cagccctggc tcatgcggtt gaggaagttc atcagcgccc
     1321 ccggccgctc cgggaactcg aagcggtaca ccatctcgtc gcttacttgc gacgagcgcc
     1381 cccccaccat gtgccggata tgcagcttcg ccatctcgtt gtccgacagg tccgcggtgg
     1441 gcagcccggc gtgacgcaag cgccggacca gctgcgcggt ctcctcgcgg ttgtgcgtgg
     1501 acagcccgac gaacacgcgc gcccgctcgg gatcgccgta gcggtagttg aactcggtga
     1561 tgttgcgcgg tcccagcagc gcgcagaagc gcttgaagct gcccggccgc tcgggaatcg
     1621 tcaccgccag gatcgcctcg cggcgctcgc ccagctcggc gcgctcggcg acgaagcgca
     1681 ggcggtcgaa gttcatgttc gcgccgcagg cgatggcgat cagcgtcttg tcccggatgc
     1741 gccggcgctc gacccaggcc ttcgccccgg cgatggcgag cgctcccgca ggctccagga
     1801 tggtgcgggt gtcctcgaac acgtccttga tcgccgcgca gatgtcgtcg ttgtccacca
     1861 gcaccatctc gtccaccagc tcgcggcaca gccggaaggt ctccgcaccc ggctccttca
     1921 ccgccacgcc gtcggcgaac agcccgacct ggggcaggcg cacgcggcgg cccgcggcga
     1981 gcgagcgcgc catggcgtcc gagtcgctcg gctgcacgcc gaggatgcgg atctcgggcc
     2041 gcaagcgctt gacgtaggcg gcgacgccgc cgatcagccc gcccccgccg accgcggcga
     2101 acagggcgtg gatcggtgcg ctgtgttcgc gcagaatctc catgccgatg gtgccttgcc
     2161 cggcgatgac gtccggatcg tcgtacgggt gcacgtacgt cagccgctcg cgccgcgcca
     2221 ggaccgtcga atgggcgtag gcgtcgtcgt aagaatcccc gtgcagcacc acgcgcgcgc
     2281 cgcgcgcggc caccgcttcc agcttgatgc ggggggtggt ggccggcatc acgatcaccg
     2341 cccggcagcc gagcttctgc gcggcgaagg cgaccccctg ggcgtggttt cccgccgagg
     2401 cacagatcac gccccggcgc agcaccggcg ccggcaggtg caccatcctg ttgtaggcgc
     2461 cgcgcagctt gaaggaaaac accggctgca cgtcctcgcg cttcagcagc aggcggttgc
     2521 ccagccgggc ggacaggttc gcggccggct cgagcggagt ctcctgagcg acgtcgtaga
     2581 cgcgggcctt gaggatgcgt tccagatagt cgggcatggg cgggcggctc cgaggtgcag
     2641 gcgagggtag cagcgccccc gcccgggatc aattgcgata ttcttcaccg ttgactctgg
     2701 aggcgacgcg ccggatgacg gcagagcaga tgaagcgggc ggcggccgag gccgcgatcg
     2761 agcatgtgcc gccgggcgcg gtggtgggcg tgggcaccgg ctccaccgtc aacttcttca
     2821 tcgaggcgct gggccggatc aagggccgca tcgagggtgc ggtggcgagc tccgaggcct
     2881 ccgccgagcg cctgcgccgg ctcggcatcc cgctgctcga gctgaacagc gtggaacggc
     2941 tgccgatata tgtggacggg gccgacgagg tcacgcgccg cctgcacatg atcaagggcg
     3001 gcggcggggc cctgaccggg gagaagatcg tggccgcggt ggcgcggcag ttcgtgtgca
     3061 tcgccgacga gggcaagctg aaagaggtgc tgggcgcgtt tgcgctgccg atcgaggtga
     3121 ttcccctggc gcgcgcccac gtcgcgcggg agatggcccg gctggggggg caggcgcggt
     3181 tgcgcgaggg attcgtgagc gactatggca acctcattct ggacgtgcac gggctcagca
     3241 tccgcgaccc ggtcgagatg gagacggcga tcgacggcat cgccggggtg gtgaccaacg
     3301 ggctgttcgc ccgccgccgc gcggacctgt tgttgctggg cacgcccttg ggggtgcgca
     3361 gcatcggcgc ctgaggccgc cgcggcctga cgcaggcgcc gccgggccgt ggctgcaaca
     3421 gctttgtcaa gttggcgcgg tagactggcc ggcccgatcc cggggagcgg cccgctggcc
     3481 gcaaggagaa gcagcagatg aacacggaac acacccacac ctccaagcag ttcgacgccg
     3541 agctggagaa cctgcgctcg cgcgtgctgc agatgggcgg cttcgtcgag cagcagatca
     3601 cgctcgcggt ggacgcgctc acctcgggcg atctcgagct gtgtgccagc gtggtgggca
     3661 acgaccacaa ggtcaacgcg atggaagtag gcatcgacga ggactgcagc accatcatcg
     3721 cgcgccgtca gcctacggcg tcggatctgc gcctggtgat cgcggtgatc aagaccatca
     3781 ccgacctgga gcgcatcggc gacgaggcgg ccaagatcgc gcgcatggcc aagctgatca
     3841 actcggtgga ccgcgtgcag cagccgcgct acgtcgagat ccggcgcatg gcgagcctgg
     3901 cgctggagat gctgcgcaag tcgctcgact gcttcgcccg gctggacgtg tccggctcgg
     3961 ccgaagtggt gcgcaccgac cagcacgtgg acgacgagtt ccgcgccatc ctgcgccagc
     4021 tcatcacctt catgatggag gatccgcgca ccatctcggc ctcgatcgag atcctgttca
     4081 tcgccaaggc gatcgagcgc atcggcgacc acgccaagaa catctccgag tacgtgatct
     4141 acatggtcaa gggcaaggac gtgcgccacg tcaccctgga ggagatcgag cgcgagatca
     4201 tggcgtgacc cagctcagca cgctcgccgc cgtcgatctc ggctcgaaca gttttagact
     4261 gcaggtcgcc cgcgtcgtcg acgaccagat ctatccgctc gactcgctgc gcgagccggt
     4321 gcggctggcc gcgggcatca ccgccgacaa gcgcctcgac gaggcctcgc agcagcgcgc
     4381 gctggatgcg ctcaagcgct tcggcgagcg cctgcgcggg ctgccgccgg aggcggtgcg
     4441 cgcggtgggg accaatgcgc tgcgcatggc caagaacgcg cgcgagttcc tggtcaaggc
     4501 cgaggccgcg ctcggtttcc cgatcgaggt ggtggcgggc aaggaagagg cgcggctcat
     4561 ctacctgggc gtggcgcaca gcctgccggt cactggcgag cggcgcctgg tggtggacat
     4621 cggcggcggc tccacggagt gcatcatcgg tgcgggctat cgcccgcaga ggctggagag
     4681 cctgtacatg ggctgcgtca gctacagcct gcgtttcttt cccggcggca agctcaccaa
     4741 gggcaacttc aagcaggcgg agaccgcggc gcggctcgag ctgcagacca tcgcacgcga
     4801 cttctccgcc gggcgctgga aggaggcggt cggctcctcc ggcacggcgc gcgcgctcgg
     4861 cgacatcctc gaggcgaacg gctggagccg gggcggcatc gccgccgagg ggctcgagcg
     4921 gctgcgcagc gcgctgctga aagcggccga gctggaccgc ctggatctgc cgggcctgcg
     4981 gcccgaccgg gtgccggtgc tgcccggcgg cttcgccatc atggcagcgg cgttctcggc
     5041 gctgcgcatc gagcacatga cgctcgccac cggcgccatg cgccagggtg tcctgtacga
     5101 cctgctcggg cgcttccacg atcgcgacat gcgcgacagc acggtggcgc agttcatgaa
     5161 gcgctatcac gtcgacgccg gccaggcgcg ccgcgtcgag tcgctggccc agtctctgct
     5221 gcgccagttc tccggcgctc tacaggccga gcacggctac ccgctgcatc tgctgtcctg
     5281 ggcggcgcgg ctgcacgaga tcggtttcag cgtggcgcac tccggctatc acaagcattc
     5341 ggcctacatc ctggcgtatg ccgacatgcc cggcttctcc aggatggagc aggcgcacct
     5401 gtcgctgctg gtgctggcgc atcgcggcac catggagaag atgaagggcc agatcagcag
     5461 ctcgctcgat ctggccatgg tgatggcgct gcgccttgcg gtgctgttcc accgcagccg
     5521 cagcgacgtt cgcctgccgc cgttcgaggc gcggtgcgag ggcaagcgct ttcagctcgg
     5581 tctggacgcc gcctggctgg cagccagccc gctcactgcg gccgccttga ccgaagaggt
     5641 caaggagtgg cgcaagctgg gcgtggctct agacatcgct gcgctcgagg aagtcgaagt
     5701 cgagggcgag ctgctgccgg ccgactagcc cggatatttc atcaggacgc ggaattatgg
     5761 cccgttctga acgagtcaga tcgggctcga ccgccgcagc caatagatca ttctatgcct
     5821 gcttgggggg cccgctactc gtacgtgcgc agcctgttta cgtccggcac gtgcacgctg
     5881 cgcccgtcga cgcggatcag ccccgcctcc gtcagctcat gcaggatgcg tgagaaatgc
     5941 tgcggcgtga ggttcaaacg cgaggcgatc acgcccttgt tggtaggcag tgtgatgctc
     6001 accgccctgg gtccgccctc cgccagggtg tcgtcgcgca gtagatagcc gatcacgcgc
     6061 tgcgcgccgg agcgcaggct gtacgcctcc acgtccgata ccaggccgtg cagtcgccgg
     6121 ctcaacccgc ccagcatctt gcgcgcgaac tgcgggttgc gctcgacctc ctggaacacg
     6181 gcgttgcgcg agacgtgcag cagcatcgag tccgccagcg cctgtgcgta caccgggtaa
     6241 ggcttctcca ggaacatcac cgcctcgccg aagctcatgc ccggcccgat gatttccacc
     6301 actttgtccc cgccctgcgg cgaggtgaac gcgagcttca cctggccgta caccaccagg
     6361 tagaaaccct ccgccggatc gcccttgtgg aacaggacct ggccgcgctc cacgtgcacc
     6421 gcacgggtgc cctgcgcgac ccgctcgatc tcctcggagt cgatctcctt gaacagcggt
     6481 aggttggcca ggaacgcgct gaccttgatc tccggcttca cgagcgtgcc gtgcggtgcg
     6541 gacggtgatc attctgccac aggcggctca ggccgcagcg aggcgtgatg tgcggcaacc
     6601 agcaccagca gcagcatcac ggcgcagaag ttgtgggcca gcaccaggac gagcggcaac
     6661 tccagcgcca cggccagagc gcccagcgcg acctgcaatg ccagcaagcc cgcgagtgcg
     6721 aaggcaaggc cgcgccgcgc ccgccgcagc agcgtcgcga gcgccaggac cggggccgcg
     6781 acggccgccg cggcgtagcg atgggcgaga tgaatcgcgg cgctgggccc ggtgcgagcc
     6841 gacagcggcc gcgtcggatc gaactcctcg acggaccagg taggcggcca ccaccggcca
     6901 gcgcagtccg gcagggacgt gcaggccagc gcggaataag tcgtgctgac catcgccccg
     6961 agcgcgagct gcgcggcgag ggcggcaagc gccaaggcgg acacccaggg tagccagcgc
     7021 ggcaccatcg cctgcgcgcg cgcccacctg cccaaggcga gccaccatag cagcgccgcc
     7081 atcagcatcc cgcccagcag gttggcggtg gcgatcgccg gcgactgcgc ggtcggtgtt
     7141 gcgcgcccca gcagcgccag tccgagcgtg agcgccagca gcgccgtggc cacgccgcgt
     7201 tcacgggcca gccggcggcg gttgacgaag gcgaccagcg cgatggcgaa cactgccgcg
     7261 ccggccgcgg ctgcggacag ccgatgcagc aggcgcgcca cgggcatggg cgatgcgttg
     7321 cgccccaggt cgcgcccgta gcaggcgggc caatccgcac agcccagccc ggcggcggtc
     7381 aagcgcaagt acgcgctgct ggtggtgact agcagcacca gcagcacgcc ggccagagcg
     7441 atgcgcgcga tcaggcgctg cggtgcggcg gcttgtgctg ctgtttgggc ggccgatgcc
     7501 ggcatcgcga cagtgtgcct ccgccggcag gcgtgctcac ttggtgcgca gcgcgtgcgc
     7561 ggtccacagg ctgagcgcaa acagcagcag cgctcccgcc tggtgcgagg ctcccaatac
     7621 caccggcact ccctgcagca gcgtggcgat gcccagcccc agctgcgcga acagcatggc
     7681 gaccagcagg ttggacgcaa gccgcgcgct gcgcggcagc tgcagccggc gcgacgcgaa
     7741 ccacagcgcc ggaatcagca ggaacagcgc ccaggcgatc aaccggtggt tgaactggac
     7801 cgtggccatg ttgtgcagga agttctgcca ccagggctgg agcatcaggt actcgggcgg
     7861 gaagaactgc ccgttcatga gcgggaaggt gttgtaggcg tagcctgcgc gcgtgcccgc
     7921 taccagtgcg cccgacagca ccatcacgaa gagcagcgcg gatacccagc cggcatagcg
     7981 cgcgagtctc gcccgtgggg ggtggagcgc atcgctcctg ccgcccgcca gcagatccag
     8041 ggccgtccag aacatcgcag cgaagatcag gaaggcgagc ccgaggtgga tcgacaggcg
     8101 cacgtggctc acgcgcggat cgtcgatcag gccgctcgcc accatccacc agccgaccgc
     8161 gccttgcagc ccgcccagca ggaagatgcc cagcagccgc gggagcagcg cgcgctccac
     8221 ccgtccggtc agcgcgaaga acaccagcgg caggaagaac accagtccga tcaaacgccc
     8281 gagcaggcga tggaagtact cccaccagaa gatgcccttg aactgctcca gagacatgcc
     8341 ccggttcacc tgtcggtact cgggcgtctg ctggtactcc tggaacagct gctgccaatc
     8401 ggcctgcgac agcggcggga tcgcgccgat cagcggctcc cactccacga tcgacagccc
     8461 cgagcgggtc agccgcgtga ctccacccac caccaccatc gcaaacacca gcgcacagca
     8521 caccagcagc cagcccgcca cctgcctgcg cgcgcgtgcg gcggccgatt gcggtgcacg
     8581 cgcagataag gaaaagaggg tctgttgcat agcgcgcatt gtagtccagg ctttcccgcc
     8641 cgccgtcata tcgcgcggga aaaactgaac ctgggtgaat tccttgcccg ggaccgcggc
     8701 gtagagtctg tcgcaagcgt ggggcagcag tgcccgcgct cgaccattca tccacgcaca
     8761 ggcggacacg accatggcgg aaaccgtccc gacgccggcc caagacgagg ccgaagagcg
     8821 catcccggcg atgcagcacc tgctcgacaa ccacttcctg ctgctgttcc tgggggtggt
     8881 gatcccggcc gtgctctaca tcctgtgggg cgtgatggag atcgtcacgc tgcccaacgt
     8941 tccacagtag gcgcgcgcca cgagggagac gaccatgagc tcaatccttc cgcccccgca
     9001 acgcatctgg tggaaagagc cgatcgagaa gatcgagctc gcctggatcg ccatcgcgct
     9061 ggtgtgggcg ctgatcctgt tcttcatgat gccgtactgg cacgtgttcg gtaagcagaa
     9121 cctgtccaac gaggcctatc gcatctcgcc cgcgcagttc caggccaaga cccaggagat
     9181 ggtggaccag tacacggtgc gcacggaaac cgagcagaac gtgccggtgg tgcatccgcc
     9241 ggccggcagc gacgtgtacc tgattgcacg tctgtggtcg ttctggccga tcctggagct
     9301 cgagcagggc aagagctacc gcctgcatct ttccgcgatg gactaccagc acggcttctc
     9361 gctgcagccc gagaacgtca acctgcaggt cgtgccgggc tacgagatgg tgatcaccat
     9421 ctcgcccaac aagaccggca gtttcgcggt ggtctgcaac gagtattgcg gcatcggcca
     9481 tcatcagatg gtgagccgga tctacgtcaa gtaaggggga aggacatatg gctgcgtcat
     9541 tccgcatcga tccgcgctcg ggcctgcagt accacgacca ggccgaggcc ctgatcaaag
     9601 ccaacgccgt ggccggcgtg gtgttcctgc tggtgggcgg catcctgtcg ctgctggtgt
     9661 cgctcacgcg ttggccggcg gtgcacctgc tggacgccaa ctggttctac ctggcgctca
     9721 ccgcacacgg catcgacatg ctgatcttct ggatcatctt cttcgagatc gcgatcctgt
     9781 acttctgctc ttccaccctg ctgcgctgcc ggctggcgac gccgcgctgg gcgtgggccg
     9841 gcttcgccct gatggtgatc ggctcggtga tgaccaacat cgccgtgttc cagggcgagt
     9901 ccagcgtgat gatgacctcc tacgtgccga tgcaggccaa gcccgccttc tacctcgggc
     9961 tgatcctgtt cgccgtgggc gcgctgattg gctgcttcgt gttcttcggc acgctggtga
    10021 tcgcgcgcgc cgagcgcacc tacgagggct cggtgccgct ggtgaccttt ggcgcggtca
    10081 ccgccgccat catcgcggtg ttcaccatcg cctcgggcgc catcatcctc attcccacct
    10141 tcctctggtc gatcggctac atcagcacca tcgatccgct catgtaccgg gtgatctggt
    10201 gggggatggg acactcgtcg cagcagatca acgtcgcggc gcacgtggcg gtgtggtacg
    10261 cggtcggcgc gatcgtgttc ggcgccaagc cgatgtcgga gaaggtgagc cgcatcgcgt
    10321 tcctgctcta tatcctgttc ctgcaactcg cctgcgctca ccacctgctg tccgatccgg
    10381 ggctgtcctc cgagtggaag atcctcaatg ccagctattt catgtacttc gcggtgctgg
    10441 ccagcatggt ccacggcctg accgtgccgg gctcgatcga ggtggcgcaa cgcaggaagg
    10501 gcttcaccaa gggcctgttc gagtggctgc gcaaggcacc atggggcaac ccgaccttcg
    10561 ccgccatgtt catctcgctg atcggcttcg gcttcctggg cggcatctcg ggcgtgatga
    10621 tgggcaccga gcagctcaac atgctgatcc acaacactct gtacgtgccg ggtcatttcc
    10681 acgcgacggt ggtcatcggc accacgctgg cattcatggg gctgacctac ttcctgctcc
    10741 cgagcctgtt caaccgtgag gtggcctttc ccggcgtggc caagtggcaa ccctatgtgt
    10801 tcggcatcgg catgtacgtg ctggcgctca cgctgatggg cgccggcacg ctgggggtgc
    10861 cgcgccggca ttgggacatc ggcttcgccg gcaccacgct gccttacgag ttcccgggtg
    10921 ccgcctactt gatgctggcg ctggtggggc tgagcgcggt gctcgcggtg ctcggtggcg
    10981 gcatgtacat cctggtcacc gtgtggtcga tcctgttcgg caagcgtctc gagcccggca
    11041 gccgcaatac gcgtcccagc cccctcaggc tgcagccggt ggcggccgcc atcgaagggc
    11101 acggcggcgg tatcggcgtg ctgcaggcgc gcgggacctt caccatggcc atggtgttcc
    11161 tggtggcctt cgtcctctac tacttcatca actggaagta cctggggtcg gtgtggggat
    11221 tgagctaggc gcaagcgtga gggatgaaac gtacctgcgc gcggcgggca actggagggg
    11281 atgcgcatgc cagcgcaatt ggccatgacg gtgccacgga tggaccgatt cagacttgtc
    11341 ctcggcctgt tcaagctgcg catcggcgtg gtgatcgcgc tcaccgctgt ggccggctgg
    11401 gcggtggcca gcggtccgcc gctcgccgcg tggcaggtgg cggtcatggc tgtggccgtg
    11461 ctgctgtctt cggcgagcgc gggcgccttc aaccagtacg tcgagcgcga catcgacgcg
    11521 cgcatgaagc gcactcgcca acgcgccttc gtcaccggcg cgctgccgca ctcgcgcgcc
    11581 tggctctggg tgatcctgct gctgctcgtc ggctcggtgg cggcggcggc gtgggccacc
    11641 aacgcgatgt cggcgctgtt cgtcttcctc ggtgctttct tctatgcggt ggtctacacc
    11701 gtgtggttga agcgccgcac ttggctcaac atcgtgatcg gcggtctggc cggcagcttc
    11761 gcggtgctgg ccggtgccgc cgcggtggac ccggccatcg gcaccgtgcc gatgctgctg
    11821 gcggtggtgc tgttcctgtg gacaccgccg cacttctgga gcctggccat ggcctgccgg
    11881 caggactacg aagcggcggg tgtgcccatg ctgcccgtgg tgatgggcga tgcgcgctgc
    11941 gcccgagcga tcttcgcgca cacgctcgtc ctggtgtcgc tgtcgctggt gctggcgttc
    12001 ctcggattgg ggccgctgta cctggtcgcg gcgctcggcg gcggcgcgtg gttcgtgcgc
    12061 gcgagctggc ggctggtgcg cacacccggg cccaaggcgg cgatgatcaa ctttcatgct
    12121 tcgctgctcc agttgagcct gctgctgctg gcggccatcg tggacggcgc catgctgtga
    12181 tcgcttctgt ttccagccgc ctgcgcgccg cgctatgcgc gggcgcggct gccgcgatgc
    12241 tgtttccggg agcggctcgc tccggggttt ccgcgctgcc tgctcttcag ccctctcccg
    12301 ggcagcgggc cgcggcgccg agtccgcccg ccctcgatcc gggcctggcg ctgacgctca
    12361 gccagcgcgc gctcggtgtc caggtcgggg agcacgtcct gcacgatacg cagggccggg
    12421 cggtgccgct gtccagcttt cgcggcaagc cgctcctgat ttccttcgtt tacaccggct
    12481 gcttcacggt ctgtcccacc accacgcgca ctctcaagcg cgcggtcgaa tcggccgtcg
    12541 gcacgctggg cagcgacagc ttcaacgtcg tgagcatcgg cttcaacctg ccgttcgaca
    12601 cgccgcaggc gctgcaagcc ttcgcgcatc agcagggcgt taccctgccg aactggcact
    12661 tcctcagccc cgacccggcg actctgcagg cgttgacgca cgagctgggc ttcagttacg
    12721 cgccgctggc gggcggcttc gaccatatca cgcaggtcag cgtcgtcgac cagagcgggc
    12781 gcgtgtaccg tcaggtgtac ggcgagcgct tcccgctgcc gcagctggtt ggcccgctga
    12841 aggagctgat caccggcgcg ccgccggcgg agcaatccct ggcgggcctg ctcgaacgcg
    12901 tgcgcctgtt gtgcacgtat tacgatgccg acaccggtcg ctaccgctat cgctactcgc
    12961 tgctgctgga gatcaccggc gggctgcttg gcatctcgac catggcggcc ttcctgctgt
    13021 acgaactgcg caagacgcgc cgcgccgccg gcccttaacg caggttaatt cacgtctcgg
    13081 agcgcgcgcg taggatggtc tacatgcgcg cgcccgaccc tgaccgtggt tcccttctgc
    13141 gcgacgcgct gcagcgcgtc gaggcgacgt tcgaccgggc gttcggcccg cgcgccaacc
    13201 cctggcgaca tctgggtgcg ctcggcttcc tcctgttctg gatcgtcggc gtcagcggtg
    13261 tgtacctgta cgccctgttc gacaccagcg tgcagggcgc ctacgcttcg gtcgagcact
    13321 tgtcgcgcga acaatggtgg tacgggggca tcgtgcgcag tgtgcatcgc tacgcctccg
    13381 acgcgttcgt gctggttctg ctgctgcact tggcgcgcga atggatccac ggccggcatc
    13441 gcgcgttccg ctggttcacc tgggtgagcg gcgtaccgct gctgtggctg gcgctcgccg
    13501 cgggcatcgt cggcttctgg ctggtgtggg acgagctggc gcagttcagc gccatcgcca
    13561 ccgcggagtg gctggactgg ctcggtctgt tcgccgagcc gctcacgcgc aacttcctgc
    13621 gccccgacag cgtggacgac cgtttcttct ctctgctggt gttcctgcac atcggactgc
    13681 cgttgctgct gctgctcggc atgtgggtgc acatcaagcg cctcgcccat ccggatacgc
    13741 gtcccggcgc ggcgctcacc tggggcacac tggcggggct gacggcgctg tcggtcgcgc
    13801 tgccggtgca aagcggcgcg ccggccgacc tcgctctcgc accgcacgcg ctcgccatcg
    13861 actggttcta cctcgcgccc aacgcggccg tgtacgcgtg gtcgccaggc gcgctatggc
    13921 tggtcgcggc cggcgtgacg gtgctgctgg tcaccttgcc gtggctgccg cgcgcacgcc
    13981 cgcagccggt tgcgaaggtc gatccggcca attgcaacgg ctgcggacgc tgcttcgtcg
    14041 actgcccgta tgccgccatt accatcgaac cgcgcatcat cggaaccatc gagccccgca
    14101 ccatcggtgg cgtcgctccg cgcgccaacg ccatcctcga cacccgcaat ggcggcaggt
    14161 gcgaacccgg gctggccgtg gtggatccgg ccttgtgcgc ttcctgcggc gtgtgcgccg
    14221 gttcctgtcc gtcgtccacg ccgtttcgca gtgtggccga gctcatcacc ggcatcgaca
    14281 tgccgcagct tcccatcggg cagctgcgca gccggctcaa atcccagatc gcgcgcttga
    14341 agggtgagcc gaagctggtg gtgttcggtt gcgacaatgc ggtcgacgtg cgcgcgctgc
    14401 agcgcgcgga cacggcggcc atgaacctgc tgtgcagcgg acaactcccg ccggctttcg
    14461 tcgagtacgc gctgcgtcag ggagcggacg gcgtggtggt caccggctgc ggcgagtgcg
    14521 attgcgcgtt ccggctgggg aatgcctgga ccgagcaacg actgcgcggg cagcgcgagc
    14581 cgcacctgcg ccgcaccgtg tcgcccgccg agctgcgcgt gatatgggcg agcgcgcagc
    14641 agcgcgaccg ggtcgtgcac gcggtggacg ccttccgcgc cgagctggct gcacgcggcc
    14701 gcgccgccgt cagcgctgcg ggtaacggct atgcgtaacc tcgtcgcgat cgtgggtcag
    14761 gcgctgctgt acgcggcgtt cgccgcctgc gtcgggctgt tctcgatgtg gccgccctac
    14821 cggcatctcg agcccggcca ggccctgctc aagttgtcct tcagccatgc cggcgaacgc
    14881 gtggcggaat gccgcaggcg cacgccgcag gagctcgcca agctgccgcc gaacatgcgc
    14941 gcgccgctcg actgtccgcg cgagcgctcg ccggtgagcg tcgagctgaa gctggacgga
    15001 caggtcatcg cgcggcgcgt ggtctcgccc tcggggttgg ccagggacgg ggcggcgacg
    15061 atgtacgagc gctttccggt gcccgccggc gcgcatcgcc tggaggtcga ggtggacgac
    15121 agcgtgcgcg ccccgggccc gacctgggaa cgcgagcagg acgtgcggct tgcgcccgga
    15181 cgcgtgctgg tggtcgatct cgatcccgag aagggaggca tcgtcatcca atgaagctcc
    15241 tgcctctgcc atcctccgtg cgcgcggggg ccatgcgcgc ggcgcatccc gccgcggccc
    15301 accctgccgc ctaccggctg gccgttcccg ccgacggccg ccgcacgctg gcggcaggct
    15361 ggctgttcct gggcatcggc gcgctgatcg cctcggggct gttctccgtg ctgctggtgc
    15421 tgtcgcgcac gcccggactg aaggacctgt tcccggtggc ggatttcttc cgcgtcgcgc
    15481 tggtggtgca tgtcgatttg tccgtgctgg tgtggttcgt cgctttcgcc ggcgtgttgt
    15541 ggagtctggc gggcacgcgc caaggcgcgc ggctggcgtg ggccggtttg gggttgtgtg
    15601 cggtgggtgc gctggcgctg agccttgcgc ccttcctcga gcgcgcgccg gccatcatgg
    15661 cgaactacat tcccgtgctg gacggcccgg tgttcctcgg cgggctcctg gtgttcgccg
    15721 cgggcgtcgg cctcgcggtg ctgcgcgcga tgttcgccac gcagccggtg ctgcacgttc
    15781 tcgacgggcc ggcggcgatc cgtctgggcc tagtcgccgc ggcggtgtcc actgcggtgg
    15841 cgctgttcgc tttcctctgg tcctggctgg cgctgcccgc cggcctggaa ccacgcacct
    15901 attacgaggt gctgttctgg ggcggcgggc acgtgctgca gttcacctgg acgctgctgc
    15961 tgctggtggc ctggctgtgg ttggccgacg cgatcggggc gcggctgccg ttgagcccgc
    16021 gcgtggtcct ggtcctgttc gggctggcgc tgctgctggc gttcttcacg cccttcgtct
    16081 atctggcttt cgacgtcacc tcggtcgagc accggcgcgc gctcacctgg atgatgcgcg
    16141 tgggcggtgg gttcgccatt ctgccggtgg ccttggcgct ggtgctggcg ctggcgcaga
    16201 tgcgcgatat cgcgcagcgc cggcgcgcgc cgcgcgcggc cctggcgatg tcgctcacgc
    16261 tgttcgcggc cggcggtctg atcggcttcg ccatcgacgg ttccaacgtg aaagtgcccg
    16321 cgcattacca cggctgcatc gtcggcatca cgctcgcctt catgggcgtg gcctacgagc
    16381 tgctgccgcg tttgggtttc cgcgcgccgg tgtcgcgcct ggcggtgtgg caaccggtga
    16441 tctacggcac gggccagctc atgcacatcg gcggcctggt gtggtccggc ggctacggcg
    16501 tgcaacgcaa ggtcgcgggc gcggaccagg tgttgaccgg tctgcagcag gtggcgggca
    16561 tggggttcat gggtctgggt ggcctgatcg cgatcgtcgg cggcgtgctg ttcctgcttg
    16621 tggtcgcgcg cgcggtgtgg cccgcgcgta cgccactcag gggcgcattg gcatgagact
    16681 ggccgcatga acgatcgcga gccgctcgac gtgcgctggc tcgagccgcc cgaaccgttc
    16741 gagcgcatcg tcgccgcgct ggaggcactg ccggccggcg agacgctgac cgtgatgatc
    16801 caccgggagc cgcatccact gtaccgctgg ctaacgcgcg agggctggcg ttacaacacc
    16861 cgctacgacg cggccggcta cttcgagatc cggatcggcc gctgaaacgc gcgcagggtg
    16921 aacctcggac tgccacatca cggcatagcc cggcgccggg cgcacccgac ctcattctag
    16981 aaggagcagt catgcaattg cgaaccctct ggcgctggcc cgcgctgtgg ctcgcgaacc
    17041 atccggcgct ggcgcaagcg tgctgatcca acgcgccggc caggcgctct tcctgcaggt
    17101 ggaacggggc ttcaacgccg cgttcggcga ccggctgaac ccgttctacc atctgggcga
    17161 gatcagcttc ttcctgttct gggtggttgc cgccagcggg ttgtacctgt acgcgttctt
    17221 cgacaccggc gtcagcgcgg cctacgcgtc ggtcgagcgg atcacccacg gccaatggtg
    17281 gctgggcggg gtactgcgca gcgtgcatcg ttacgcgtcg gacgcgatgg tactgacgat
    17341 gctgctgcac ttgctgcggc acttctgctt cgaccgctac cgcggttttc gctgggtctc
    17401 gtgggtgagc ggcatcgcgg tgctgtggct ggtgtacgtg tcgggcatta acggtttcat
    17461 gctgccatgg gaccggctgg cgcagttcgt ggtgacgggc agcgccgagt acctggactg
    17521 gctgccgatc ttcaacggcg cgctcacgcg caacttcatc taccagacga gcgtgaacga
    17581 ccggctgttc tcgctgctct cgttcatcca catcggcgtg ccgctggtat tgctgctgct
    17641 gctgttcgtg cacatccagc gcactcccaa ggcggcgacg gcgccgccgc gcaggatctg
    17701 ggtgccgctc acctcgacgc tgctcgtgct gtcgttgatc gtgcccgtcg tcggcgatgc
    17761 gccggcggac ctgagcgtgg taccggagcg tcttgcgttc gactggttct acctggcgac
    17821 ctttccgctc atgtacgcct ggtcggtagg cgcggcgtgg atgcttctgg gcgggatcac
    17881 gctggccttg gtgctgttgc cgtggctgcc gcccaagcgc gtgggcaggg aggggctgcg
    17941 cgccatcgtt catccgggcg accgcgagat cctggtgcgt cccgacgaaa ccattctcga
    18001 ggcggggctg cgcgcgggca tcccgatgcc attcgagtgc cgcaacggcg gttgcgggtt
    18061 gtgcaagggc gccctcctgt acggcaggat cgagcacgcg ccttaccaga agagcgcgct
    18121 gagcgacgcc gagcgcgcgg cgggcaaggt gctgttctgc tgcgccacgc cgcttaccga
    18181 gatcgagatc gagtacgaag aggccggcgc cgcccgcgac ttccccttga aggcgttgga
    18241 ggtgacggtg cggcgcatgc gccgtgtgtc cgacgcggtg atgatcgtgg acctcgcgct
    18301 gccgcccggg gagcgcatgc actatcgggc ggggcaatac ttcaacgtcg tgctcgaaga
    18361 cggcgcgcgc cgtgccttct cctttgccac cgcggcggat gatagcgatc gcatcgagat
    18421 gcacgtgcgg ctgattcccg gcggccggtt caccacccat gtgttcaccg cgatgcgcga
    18481 aggcgaccgg ctgcgcatcg agggcccgct cggccagttc gcgctgcacg acagcgatcg
    18541 gccgctgatc ctcgtggccg gcgccactgg attcgcgccg gtgaagagca tgctcgagta
    18601 cgcgttccgg gccggcctca agcgccggat gttcctgtat tggggggtac gccgccgcga
    18661 cgatctgtac atgcacgagc tgcccgagcg gtgggagcgt gagcatgcca acttccactt
    18721 cgtgccggtg ctgtccgagg cgcaaaccga ggacgactgg cacggccgca ccggcctggt
    18781 gcacgaggcg atcctgcacg acttccccga tctggccggc ttcgaggtct acacctgcgg
    18841 ctcgatgaag atggtggaag cggcgcgccc cgccttcctc gggcaggggc tcgacgagga
    18901 cgcctgcttc gccgacgcgt tcttcccgcc ggcgccgatc cagacgacct gaagtcccgc
    18961 gcgtcgttgg cttggggcgc gggccgtggc gcgcttgccg gtatgatgcg caatggccgg
    19021 caggaggaag cagccatgat gggcatcgag gtcctgcgcg attcgctcgc gaacaaaggc
    19081 accgctttta ccctcgagga gcgcaagcgg ttggagttga ccggcttgct gccggcgcgc
    19141 gccgagacga tcgacgagca gttcgcccgc gtgctcgcca acctgcgcgc caagccctcg
    19201 cccatcgaga agttcgtcta cctcgccgcc ctgcgggacg acaacgagac gctgttcttt
    19261 cgtgcgctga tcgaccacct cgaggaactg ctgccgctgg tgtacacccc caccgtgggc
    19321 caggcctgct cggagtggag ccgcatctgg cggcggccgc gcgggctgta cgtcacggca
    19381 cgcgagcatg gacgggtgcg cgaggtgctg cgccgctggc ccgcgcgcaa gatcgctgtc
    19441 atcgtcgtta ccgacggcgg ccgcgtcctt gggctcgggg atctcggcgc caacggcatg
    19501 ggcatcccga tcggcaagct tgcgctctac accgcctgcg ccggcgtgga cccggcgctg
    19561 tgcctgccgg tgatgctcga cgtgggcacc gacaccgagt cggtgcgcga cgacccgttc
    19621 tacctcggcc tgcgccagcc gcggctcgtg ccggcgcact acgacgcgct gatcgaggaa
    19681 ttcgtggccg ctgcgcagga aatcttcccc ggcgtgttgt tgcagttcga agacttcgcc
    19741 aacgtgaacg ccttcaggct gctcgcccgc taccgcgatc gcatctgcgc tttcaacgac
    19801 gacatccagg gcacggctgc gatggctctc gccggtctgc gcgcggcgca gcacctgacc
    19861 gggcggcggc ttgccgagga gcgcctgctg ttcttcggag ccggtgcggc caacaccggt
    19921 atcgccacgc tggcagtggc ggcgatgatg caggcggggt tgacgcaggc cgaggccagg
    19981 gcgcgctgct ggttcatgga ttcgcgcggt ctggtggtcg agggccgtgc cgggctggct
    20041 gcgcataagc aacccttcgc gcaggtgcac gcgccgatcc cggatttgct cgcggcggtc
    20101 gaggcgattc ggcccacggc gctgatcggc gcgagcggcg cggcgggcgc cttcaccgaa
    20161 ccggtgctgg ccgccatggc cgccgacaac cggcgaccgg tgatcttcgc tctgtccaac
    20221 ccgacttcca aggccgagtg cacggcggag caggcctacg ccgccacacg cggacgcgcg
    20281 atattcgcga gcggcagtcc gttcgcgccg gtcacggtgg aggggcgcag ccgcgccacc
    20341 ggccaggcga acaactcctt catctttccg gggctgggcc tgggcgtgct cgccgccggt
    20401 gctacgcgcg tgaccgacgc tatgttcctg gcggccgccg atgcgctggc cgggcaggtc
    20461 tccgccgacg acctcgacgc aggacgcgtg tttccgcctg ctgcgaggat gcgcgaggtg
    20521 gccgccgccg tcgccacggc cgtggcgcgc gtggcgcacg accgcggcct ggccacacgc
    20581 ccgcagccac cggacctcgg cgccgcgatc cgggagagca tgtaccgggc cgactacgcc
    20641 gcggctgggt agcacacagc agccggttcg agcgcggcag gatggaacaa gacggctctg
    20701 ccgagagttt gcgcaggcac gtggagcggc tcgcgggcga gatcggcgag cgcaacgtgt
    20761 ggcggccggc agcgctgcaa gccgccgccc gctacatccg cgagcaatgg caggcgctgg
    20821 ggtacgcggt cgcggctcag ccgtaccgca cccatggact cacctgcgag aacctcgaga
    20881 tcacgattcc gggcctgcgc cgtccgaacg agatcgtgct ggccggtgcg cactacgact
    20941 ccgtgcgcgc cagcccgggc gccgacgaca atgccagcgg agtcgcggcg ctgatcgaaa
    21001 tcgccgcgct gctgcgcggg cagaggcccg cgcgcaccct gaagctcgtc gccttcgtga
    21061 acgaggagcc g
//
LOCUS       DASPGE010000378         3287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_801215, whole genome shotgun sequence.
ACCESSION   DASPGE010000378 DASPGE010000000
VERSION     DASPGE010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801215"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VD791_12275"
     CDS             complement(<1..488)
                     /locus_tag="VD791_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV08802.1"
                     /translation="MKEFKDKVAVVTGGASGLGLAMARRFAAEGMKLVLADVEEEALR
                     RVELELRKAGVPVIGIWTDVSRSQDIERLAEKALATFGAVHVLCNNAGVAPSGAVWEN
                     TVADWEWVLGVNVWGVIHGVRTFLPIMLRQDTECHVVNTASVAGLLSVPNMGIYCVSK
                     HAV"
     gene            706..1770
                     /locus_tag="VD791_12280"
     CDS             706..1770
                     /locus_tag="VD791_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HXV08803.1"
                     /translation="MHEQFIGTMPVQERHRFDVDKLDGYLRAHVQGYRGPLQAEQFRG
                     GQSNPTFLLRTPQRRYVLRRKPPGKLLPSAHAVDREYRVISALHGVGVPVARTYVLCQ
                     DDGVIGTAFYIMEYVDGRVLWDGSLPGMQRGERHAVYDELNRVIAALHQVDYLAVGLG
                     DFGKPGNYLERQIARWSKQYRASETEKIEAMERLMEWLPQHIPPGDDTCIVHGDYRLD
                     NVIFHASEPRILAVLDWELSTLGHPLADFAYHCMAWRLKPGEFRGIGELDLAALGIPS
                     EEEYVAMYCRRTGRSAIPNWDYYLAYNMFRLAAILQGIMGRVLDGTASSQHAIESGRR
                     ARPMAEAGWRQVEKILGAQS"
     gene            1767..2741
                     /locus_tag="VD791_12285"
     CDS             1767..2741
                     /locus_tag="VD791_12285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HXV08804.1"
                     /translation="MRAVLCKELGPPEKLVVEDVPSPRPGKGQVVVSVKAAGVNFPDT
                     LIIQGKYQFKPEPPFSPGGEVAGVVIELGEGVSGVKPGDRVIASSTYGGFAEEMAIEA
                     ERLVAIPEAMDYVPASAFILAYGTSYHALKDRAHLKAGETLLVLGASGGVGLAAVQLG
                     KAMGARVIAAASSDAKLAVCKDNGADELINYSSEDLRARVKAITAGRGLDVVYDPVGG
                     PYSEPALRDMAWNGRFLVVGFAAGEIPKVPLNLPLLKGCSIVGVFWGAFTRTEAQRNR
                     RNNEELMRLYLAGKVKPHIHATYPLERAAQALNEVLYKRVSGKVVLTV"
     gene            2756..>3287
                     /locus_tag="VD791_12290"
     CDS             2756..>3287
                     /locus_tag="VD791_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXV08805.1"
                     /translation="MQSTMMQVPLNLSHFLERAGKLFGAQEIVSRMPDKSLHRYTYAD
                     FYRRARALAEALTRAGLGRGDRVATLMWNHYAHYETYFGVIAAGGVLHTLNLRLAPEE
                     IGYIANHAQDRVLIVDDILLPLFERFRGQVNLERVIVVPLTGKPVPAGMDDYEAFLGT
                     ATGEFAYPPMDENEPCA"
BASE COUNT          534 a         1119 c         1135 g          499 t
ORIGIN      
        1 acggcgtgct tggacacgca gtagatgccc atgttgggca ccgacagcag cccggccacc
       61 gacgccgtgt tgaccacgtg gcactcggtg tcctggcgca gcatgatcgg caggaaggtg
      121 cgcacgccgt ggatcacacc ccagacgttc acgcccagca cccactccca gtcggccacg
      181 gtgttctccc acaccgcgcc gctcggcgcg actccggcgt tgttgcacag cacatgcacg
      241 gcgccgaagg tggcgagggc tttctccgcg aggcgctcga tgtcctggct gcgcgacacg
      301 tcggtccaga tgccgatcac cggcacgccg gccttgcgca gctcgagctc gacgcggcgc
      361 aaggcctctt cctcgacgtc ggcgagcacc agcttcatgc cttcggcggc gaagcggcga
      421 gccatggcca ggccgagccc gctagcaccg ccggtgacga cagcgacctt gtccttgaac
      481 tctttcatgg aaagccggag cggcgtgcgc aggacgacgg acgcgggatt ctagcatgcg
      541 cgtccacgcg gccgtttcgg cacgctgtcg tattcgcatg ggcgcacttg agcgggcgca
      601 tctggacagc gcatttgcgc ggggtgcgcc cgcgcggtat cctcgcctga cccgctctgc
      661 ccgcgagcgc cggcggccgg gccacccgag cacggaactt gagtcatgca cgagcagttc
      721 atcggcacga tgccggtgca ggagcggcac cgcttcgatg tggacaaact cgatggctat
      781 ctgcgcgccc acgtgcaagg ctatcgcggc ccgctgcagg cggagcagtt ccgcggcggg
      841 cagtccaatc ccacctttct gctgcgcacg ccgcagcgcc gctacgtgct gcggcgcaag
      901 ccgcccggca agctgctgcc ctcggcgcac gcggtggacc gcgagtaccg cgtcatcagc
      961 gcgctgcatg gagtgggcgt tcccgtagca cgcacgtacg tgctgtgcca ggacgacggc
     1021 gtgatcggta ccgccttcta catcatggag tacgtcgacg ggcgcgtgct gtgggacgga
     1081 tcgctgcccg gcatgcagcg cggtgagcgg catgccgtct acgatgagct gaatcgcgtg
     1141 atcgcggctc tgcaccaggt ggactacctg gccgtcggcc tgggcgactt cggcaagccg
     1201 ggcaattacc tggagcgcca gatcgcgcgc tggagcaagc agtaccgcgc ctcggaaacg
     1261 gagaagatcg aggcgatgga gcgcctcatg gaatggctgc cgcagcacat cccgcccggc
     1321 gacgacacct gtatcgtgca cggcgactac cggctggaca acgtgatctt ccacgccagc
     1381 gaaccgcgca tcctcgccgt gctcgactgg gagctgtcca cgctcggaca cccgctcgcc
     1441 gatttcgcct accactgcat ggcgtggcgc ctcaagccgg gcgagttccg cggcattggc
     1501 gagctggacc tcgcggcgct cggcatcccg tcggaggagg agtacgtggc gatgtactgc
     1561 cgccgcaccg gacgcagcgc catccccaac tgggactact acctggctta caacatgttc
     1621 cgcttggccg ccatcctgca ggggatcatg ggccgcgtgc tggacggcac cgcctcgagc
     1681 cagcacgcca tcgagtccgg gcgccgcgcg cggccgatgg ccgaggccgg ctggcggcaa
     1741 gtggaaaaga tcctgggtgc gcaatcatga gagcagtgct gtgcaaagag ctcggaccgc
     1801 cggagaagct ggtggtcgag gacgtgccct cgcccaggcc gggcaagggg caggtggtgg
     1861 tgagcgtgaa ggcggcgggc gtcaacttcc ccgacacgct gatcatccag ggcaagtacc
     1921 agttcaagcc cgagccgccg ttctcgcccg ggggcgaagt ggcgggcgtg gtgatagagc
     1981 tgggcgaagg cgtcagcggt gtgaagccgg gcgatcgcgt gatcgcctcc agcacctacg
     2041 gcggcttcgc cgaggaaatg gcgatcgagg ccgagcgcct ggtggcgatc cccgaggcca
     2101 tggattacgt gccggcgtcc gccttcatcc tggcctacgg cacctcctat cacgcgctca
     2161 aggaccgcgc gcacctcaag gccggggaga cgctgctggt cctcggcgcc tcgggcgggg
     2221 tgggcctggc ggcggtgcag ctcggcaagg cgatgggcgc gcgcgtgatc gctgcagcct
     2281 ccagcgacgc caagctggcg gtatgcaagg acaacggcgc cgacgagctg atcaactaca
     2341 gctccgagga cctgcgcgcg cgggtcaagg cgatcaccgc cggccgcggc ctcgacgtgg
     2401 tgtacgaccc ggtcggcggg ccgtactccg agccggcgct gcgcgacatg gcgtggaacg
     2461 gccgcttcct ggtggtcggc ttcgccgccg gcgagatccc caaggtgccg ctcaacctgc
     2521 cgctgctcaa gggctgctcg atcgtgggcg tgttctgggg cgccttcacc cgcaccgagg
     2581 cgcagcgcaa ccgccgcaac aacgaggagc tgatgcggct ctacctcgcc ggcaaggtca
     2641 agccgcacat ccacgccact tatccgctcg agcgcgctgc gcaggcgttg aacgaggtgc
     2701 tgtacaagcg cgtcagcggc aaggtggtgc tgaccgtctg aggccggaga gtccgatgca
     2761 gtccaccatg atgcaggtcc cgctcaacct gtcgcacttc ctggagcggg cgggcaagct
     2821 gttcggcgcg caggagatcg tctcgcgcat gccggacaag tccctgcacc gctacaccta
     2881 cgccgacttc taccgccgcg cgcgggcgct ggccgaggcg ttgacgcgcg cgggactcgg
     2941 gcgcggcgac cgggtcgcca cgttgatgtg gaaccactac gcgcactacg agacgtactt
     3001 cggcgtcatc gccgccggcg gcgtgctgca cacgctgaat ctgcggctcg ctcccgagga
     3061 gatcggctac atcgccaacc acgcgcagga ccgcgtcctg atcgtggacg atatcctgct
     3121 gccgctgttc gagcggttcc gcggccaggt gaacctggaa agagtgatcg tggtgccgct
     3181 caccggcaag ccggtgccgg ccggtatgga cgactacgag gcgttcctgg gcaccgccac
     3241 cggcgagttc gcgtacccgc caatggacga gaacgagccg tgcgcga
//
LOCUS       DASPGE010000379         5606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_918479, whole genome shotgun sequence.
ACCESSION   DASPGE010000379 DASPGE010000000
VERSION     DASPGE010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918479"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..695
                     /locus_tag="VD791_12295"
     CDS             156..695
                     /locus_tag="VD791_12295"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HXV08806.1"
                     /translation="MIAVNEDFGAYQKANLDSLLKYSDAATEAAEQWFELNARTAKAG
                     VAEALKQVRALATAKDVQELTSLQTGFSQANAEKMLGFARAVYGWATETQGELAKLAE
                     AQIAEINRSLAASLDKAAKSAPTGSEFAFTAIKQAMSATNQALDAVSKASKQVGEMAD
                     ATVGATTGALPARKKPATA"
     gene            867..2552
                     /gene="pgi"
                     /locus_tag="VD791_12300"
     CDS             867..2552
                     /gene="pgi"
                     /locus_tag="VD791_12300"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HXV08807.1"
                     /translation="MLTELPTWKRLAAHRDEVARHHLRELFAADPQRFARLSAAGAGL
                     FLDFSKNLILDQTLPLLCRLAEEAGVPQRRAAMLAGERINATEQRAVLHTALRNRGPQ
                     PVVLDGRDVMPDVRRVLAQMRLFATRVRDGSLCGHTGKRITDVVNIGIGGSDLGPLMA
                     CEALEAFGRSDLRAHFVSNVDGAHLGRTLKRLDPQSALFIVASKTFTTQETLANARSA
                     RAWLVAHLRSEAAVASHFAAVSTNLPATAAFGIAGERVFEFWDWVGGRYSLWSAIGLP
                     IALFVGIDAFEDMLAGGHEMDRHFASAELPVNLPAILGLLGVWYANFFGARTCAVLPY
                     DQGLHRFPAYLQQLDMESNGKRTDLAGRTLDYQTGPVIWGEPGTNGQHAFFQHLHQGT
                     QWTPADFILPLASHYAAAPAVSATSGTSADLGIGAEHHRMLVANCLAQTQALMRGRTA
                     DEARAELAAQGVSGAELERLVPHKVFPGNRPTNTILLPAVTPRSLGALIALYEHKVFV
                     QGALWNVNSFDQWGVELGKQLAGRVLEDLERGAPSAGQDASTAGLIRRVTGKE"
     gene            complement(2704..3249)
                     /gene="orn"
                     /locus_tag="VD791_12305"
     CDS             complement(2704..3249)
                     /gene="orn"
                     /locus_tag="VD791_12305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HXV08808.1"
                     /translation="MAQDATHLIWIDLEMSGLKPDSDRILEVALVVTDQNLRVVAEGP
                     VRVVHQGDAVLDAMDSWNTATHARTGLTARVRASALTEAEVEDEMLAFLAQHAPPGAS
                     PMCGNSICQDRRFLARYMPRLEAYFHYRNLDVSTLKELARRWKPELVAGLAKQGKHEA
                     LADIYESIEELRYYREHFLKL"
     gene            3328..4575
                     /locus_tag="VD791_12310"
     CDS             3328..4575
                     /locus_tag="VD791_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HXV08809.1"
                     /translation="MNAFTALFLAALAVSVGVRLWLARRHIRHILAHRAAVPGAFAPS
                     ISLEAHQKAADYSCARARFGMLHSVVDAIVLLVLTFGGLLQLLDGVAGSWLPGPLPQG
                     TALIALFALVTSIIDLPLSYYRTFGIEARFGFNKMTRAMWLADLLKHGAVAAALGLPL
                     VVAVLWLMRATGAYWWLYVWLVWVAFSVFVMAVYPALIAPLFNKFSPMQDASLKGRIE
                     RLLAKCGFRSSGLFVMDGSRRSTHGNAYFTGFGKTKRIVFFDTLLQRLNEEEIEAVLA
                     HELGHFKLHHVIRRMAWTFLVSLGFLALLGWLKDEPWFYGGLGVSYPASDAMALILFL
                     LVVPAFTFLLHPLVAMYSRKHEFEADAYAAQYSSARELVNALVKLYKDNASTLTPDPV
                     HSAFYDSHPPARIRIERLQSYAS"
     gene            4613..4954
                     /locus_tag="VD791_12315"
     CDS             4613..4954
                     /locus_tag="VD791_12315"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HXV08810.1"
                     /translation="MNEVIQELRRSRCAPCEAGTPPLGAAQIQPLLAGLQGWALEDGR
                     IAKTYRFKNYHETMAFVNAAAWISHREDHHPDLLVGYNRCKVVYLTHAIGGLSRNDFI
                     CAAKLDALFDL"
     gene            4951..5316
                     /locus_tag="VD791_12320"
     CDS             4951..5316
                     /locus_tag="VD791_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08811.1"
                     /translation="MIGGTRAAASLLALLALNTPGCASKARDAGPPEAAACTQDAGPV
                     RARDYGERLRLLTQRADAYAACMSARGFVLDEAELEAELQRLEQVRNADQLGGDPQLE
                     LALRAQELRASPIVWRRGS"
BASE COUNT          856 a         1972 c         1864 g          914 t
ORIGIN      
        1 gctctgaccg gccttgcgac cggcgtccga ggcgccttat gtgcggcgca aaaaaagctt
       61 gacacgggtg ccgaaaatac tgagaatgcg tcggttgtgc gtcgcagcat aacggttgca
      121 ccagaacggc cccattcccc caaggagact cctgcatgat cgcagtgaac gaagatttcg
      181 gcgcctacca gaaggccaac ctcgacagcc tgctcaagta cagcgacgcg gccaccgaag
      241 ccgccgagca gtggttcgaa ctgaacgccc ggaccgccaa ggccggtgtg gccgaagcgc
      301 tgaagcaggt gcgggcgctg gccaccgcca aggacgtgca ggagcttacc tcgctgcaga
      361 ctggcttctc ccaggccaac gccgagaaaa tgctgggctt cgcgcgcgcc gtgtacggct
      421 gggcgaccga gacccaaggc gagctcgcca agctggccga ggcccagatc gccgaaatca
      481 accgctcgct ggccgcctcc ctggacaagg ccgccaagtc cgcccccacc ggctcggagt
      541 tcgcgttcac cgcgatcaag caggcgatga gcgccaccaa ccaggccctg gacgccgtgt
      601 cgaaggcgag caagcaggtc ggggagatgg ccgacgccac ggtgggcgcc accaccggtg
      661 cgctgcccgc caggaagaaa cccgccaccg cctgatccgg cgtctgtccc gccgacaaag
      721 ccccccggcg cgccgggggc tttgtctttg caccgtctct gcacggggcc ccgatcccgg
      781 tgagataatc gtccgccgtc tcctcgtcac gcctttcggt cacgccgtac cggtcacgcc
      841 tcttggtcac gccgcagcga tctccgatgc tcaccgagct gcccacctgg aagcgcctgg
      901 ctgcgcaccg ggacgaggtc gcccgccacc acctgcgcga gctgttcgcc gccgacccgc
      961 agcgcttcgc gcgcctttcc gccgcgggcg ccggcctgtt cctggatttc tccaagaacc
     1021 tgatcctgga ccagaccctg ccgctgctgt gccggctggc ggaagaggcc ggtgtgccgc
     1081 agcggcgggc cgcgatgctc gccggcgagc gcatcaacgc gaccgagcag cgcgccgtgc
     1141 tgcataccgc gttgcgcaac cgcggcccgc agccggtcgt gctcgacggc cgcgacgtga
     1201 tgcccgatgt gcgacgcgtg ctcgcccaga tgcgtctgtt cgccacgcgc gtgcgcgacg
     1261 gctcgctgtg cggacacacc ggcaagcgga tcaccgacgt ggtcaacatc ggcatcggcg
     1321 gctccgacct cggcccgctg atggcctgcg aagcgctcga agccttcggg cgcagcgatc
     1381 tgcgcgcgca cttcgtgtcc aacgtggacg gcgcgcacct cggccgcacg ctcaagcggc
     1441 tcgatccgca gagcgcgctg ttcatcgtcg cctcgaagac cttcaccacc caggagacgc
     1501 tggccaatgc gcgcagcgcg cgcgcctggc tggtcgcgca cctgcgcagc gaagccgcgg
     1561 tcgccagcca cttcgccgcc gtgtccacca acctgccggc gaccgccgcc ttcggcatcg
     1621 cgggcgagcg cgtgttcgag ttctgggact gggtcggcgg gcgctactcg ctgtggtcgg
     1681 cgatcggact gcccattgcg ctcttcgtcg gcatcgatgc cttcgaggac atgctggccg
     1741 gcggccacga gatggaccgg cacttcgcga gcgccgagct gccggtcaat ctgccagcca
     1801 tcctcggcct gctcggcgtg tggtacgcga acttcttcgg cgcacgcacc tgcgccgtgc
     1861 tgccctacga ccagggactg caccgcttcc cggcctactt gcagcagctc gacatggaga
     1921 gcaacggcaa gcgcaccgac cttgccggac gcacgctgga ttaccagacc gggccggtga
     1981 tctggggcga gccgggcacc aacgggcagc acgccttctt ccagcacctg caccagggca
     2041 cgcagtggac cccggcggac ttcatcctgc cgctcgcctc gcactacgcc gcagcgcccg
     2101 ctgtctcggc aacatcgggt acctcggcgg atctgggcat cggcgccgag catcaccgca
     2161 tgctggtcgc caactgcctg gcgcagacgc aggcgctgat gcgcggccgg accgcggacg
     2221 aggcgcgcgc ggagctcgcc gcccagggcg tgagtggcgc agagctggag cgcctggtgc
     2281 cccacaaggt gttccccggc aatcggccga ccaacaccat cctgctgccg gcggtcacgc
     2341 cgcgcagcct gggggcgctg atcgcgctgt acgagcacaa ggtgttcgtc cagggcgcgc
     2401 tgtggaacgt gaactccttc gaccagtggg gcgtggagct gggcaagcaa ctcgccggcc
     2461 gcgtcctcga ggacctggag cgcggcgccc cgagcgcggg gcaggatgcg tcgacagctg
     2521 gcttgatcag gcgggtcact ggaaaagagt gacccgcctg aaggcgggat cgaaaagcgt
     2581 gaccggcatt gcgtgaccga aaagcgtgac gaaaaccgtc tggtgactag aggcgcggcc
     2641 cttggcggcc atctctccta gtcacgcttc tcgatcacgc ctttcggtca cgcctttcga
     2701 tcctcacagc ttcaggaaat gctcccggta atagcgcaac tcctcgatcg actcgtagat
     2761 gtcggccagc gcctcgtgct tgccctgctt cgccaggccg gcgaccagct ccggcttcca
     2821 gcggcgcgcc agctccttga gcgtgctgac gtcgaggttg cggtaatgga agtaggcctc
     2881 cagccgcggc atgtagcgcg ccaggaagcg gcggtcctgg cagatggagt tgccgcacat
     2941 gggcgaggcg ccgggcggtg cgtgctgagc caggaacgcc agcatctcgt cctcgacctc
     3001 ggcttcggtc agcgccgagg cgcgcacgcg ggcggtgagg ccggtgcgcg cgtgggtcgc
     3061 cgtgttccag gagtccattg cgtcgagcac ggcgtcgccc tggtgcacca cgcgcaccgg
     3121 tccctccgcc accactctca ggttctggtc ggtgaccacc agcgccacct cgaggatgcg
     3181 gtccgagtcg ggcttgagcc cgctcatctc caggtcgatc cagatcaggt gggtcgcgtc
     3241 ttgtgccata attttccgag aattttccct gtgcaattgt gtcatatcgg cggtccccgg
     3301 aaacactgaa tcgtcgccat cgcgcccatg aacgctttca ccgccctgtt cctcgccgcc
     3361 ctggccgttt cggtcggggt acggttgtgg ctcgcccgcc gccacatccg ccacatcctg
     3421 gcgcatcgcg cggcggtgcc cggcgccttc gcccccagca tcagcctcga ggcgcaccag
     3481 aaggccgccg actacagctg cgcgcgtgcg cgcttcggca tgctgcactc ggtggtcgat
     3541 gcgatcgtgc tgctggtgct gaccttcggc ggtctgctgc agctcctcga cggcgtggcg
     3601 gggagctggt tgcccgggcc gctgccgcag ggcacggcgc tgatcgcgct gttcgcgctg
     3661 gtgacctcga tcatcgacct gccgctcagc tactaccgca ccttcggcat cgaggcgcgc
     3721 ttcggcttca acaagatgac gcgggcgatg tggctcgccg atctgctcaa gcacggcgca
     3781 gtggccgcgg cgctgggtct gccgctggtg gtggccgtgc tgtggctgat gcgcgcgacg
     3841 ggcgcctact ggtggttata cgtgtggctg gtgtgggtcg ccttcagcgt cttcgtgatg
     3901 gcggtgtacc cggcgctgat cgcgccgctg ttcaacaagt tctcgccgat gcaggacgcc
     3961 tcgctcaaag ggcgcatcga gcggttgctt gccaagtgcg ggttccgctc gagcggcctg
     4021 ttcgtgatgg acggctcgcg ccgcagcacg cacggcaacg cctatttcac cggcttcggc
     4081 aagaccaagc gcatcgtgtt cttcgacacg ctgctgcagc ggctgaacga ggaagagatc
     4141 gaggcggtgc tggcgcacga gctgggccac ttcaagctgc accacgtgat ccggcgcatg
     4201 gcctggactt tcctggtgag cctgggattc ctggcgctgc tcggctggct caaggacgag
     4261 ccctggttct acggcggcct gggcgtgagc tatccggcga gcgacgccat ggcgctgatc
     4321 ctgtttctgc tggtggtgcc ggcgttcacc ttcctgctgc acccgctggt ggcgatgtac
     4381 tcgcgcaagc acgagttcga ggccgacgcc tacgccgcgc agtacagctc ggcgcgcgaa
     4441 ctggtcaacg cgctggtcaa gctgtacaaa gacaacgcct cgacgctcac cccggatccg
     4501 gtgcactcgg cgttctacga ttcgcacccg ccggcgcgca tccgcatcga acgattgcag
     4561 agctacgcat cctgacccgg aggccggaca tggacccccg ccggaggccg acatgaatga
     4621 ggtgatccag gagttgcgcc gcagccgctg cgcgccctgc gaggccggta cgccgccgct
     4681 gggcgccgcg cagatccagc cgctgctcgc cggcctgcag ggctgggcgc tggaggacgg
     4741 ccgcatcgcg aagacctacc ggttcaagaa ctatcacgag accatggctt tcgtgaacgc
     4801 cgccgcctgg atctcgcatc gcgaggacca ccatcccgat ctgctggtgg ggtacaaccg
     4861 gtgcaaggtg gtgtacctta cccacgcgat cggcgggctg tcgcgcaacg atttcatctg
     4921 cgccgcgaag ctcgacgcgc tgttcgacct gtgatcggcg gcacgcgcgc ggccgcgagc
     4981 ctcctcgccc tgctggccct gaacacgccg ggttgcgcct ccaaggcgcg cgacgcgggg
     5041 ccgccggaag cggcggcctg tacgcaggac gccggcccgg tgcgcgcccg cgactacggc
     5101 gagcgcctgc gcctgctcac gcagcgcgcc gacgcgtatg cggcctgcat gagcgcgcgg
     5161 ggcttcgtgc tggacgaggc cgagctggag gcggagctgc agcgcctgga gcaggtgcgc
     5221 aacgccgatc agctcggcgg cgacccccag ctcgagctcg cgctgcgcgc gcaggagctg
     5281 cgcgcttcgc ccatcgtctg gcgccggggc agctagcccg gatatttcac caggccaccc
     5341 ccgcatcgca tgccattgcg caccagcagg cgttctcgca gcatgcgttc tgcggaacac
     5401 tttggcttcg gggttacgcg gattctggcc tgttctggac ggccatctcg ggcatgcccg
     5461 ccttgccaat agaatgatct attggctgcg gcggtcgagc ccgatctgac cgtccagaac
     5521 aggccataat tccgcgtcct gatgaaatat ccgggctagc ctgcggcaca gccggcgacg
     5581 cacggcttcg cgcgcgccgc cgcgct
//
LOCUS       DASPGE010000380        14045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_99409, whole genome shotgun sequence.
ACCESSION   DASPGE010000380 DASPGE010000000
VERSION     DASPGE010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14045
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99409"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VD791_12325"
     CDS             <1..199
                     /locus_tag="VD791_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HXV08812.1"
                     /translation="VWMRPDDHQLMMPLYQVVFTRAGQPGVKYDAEDTGFGWKTEGRL
                     DARDVITPPTCKVQRPERQGQ"
     gene            complement(287..1375)
                     /gene="amrS"
                     /locus_tag="VD791_12330"
     CDS             complement(287..1375)
                     /gene="amrS"
                     /locus_tag="VD791_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HXV08813.1"
                     /translation="MSEANYPGRWWHMLDDGRMQCDLCPRDCRLHEGQRGACFVRRRV
                     GDAMLLTTYGRSSGFCIDPVEKKPLNHFYPGSSILSFGTAGCNLACKFCQNWDISKSR
                     DMDRLMDQASPALIAEAAYRTGCKSVAFTYNDPVIFAEYAMDVADACHARGVQTVAVT
                     AGYMHDAPRREFYAKMDAANVDLKAFTEEFYFKLTGSHLQPVLDTLVYLKHETDVWFE
                     ITTLLIPTKNDSDEEITAMCRWIARELGTDVPLHFTAFHPDWKLTDLPATPPATLTRA
                     REIAIREGLQYVYTGNVHDTTGGTTFCPGCGEALIVRDWHTLLEYRVTHDGRCAGCSA
                     PVAGRFEEYRGQHGRRRIPVRLSTFLPA"
     gene            complement(1372..2745)
                     /gene="amrB"
                     /locus_tag="VD791_12335"
     CDS             complement(1372..2745)
                     /gene="amrB"
                     /locus_tag="VD791_12335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HXV08814.1"
                     /translation="MTATIRPAAVAGAFYPADPAVLARDVAALLDRAQARLEPGQPVP
                     KAIIAPHAGYIYSGPVAATVYARLVPFAGRIRRVVLLGPAHRVAVRGLALPGATAFET
                     PLGSVPVDEEGHALLQDLHQVVVSREAHALEHSLEVHLPFLQKVLGDFKLIPLVVGRA
                     APEEVAQVLERLWGGDETLVVVSSDLSHYLGYAQAQATDRDTARLILNRQPTLDPHQA
                     CGAAPANGLLLMAARRGLGPRLLDLRNSGDTAGDRSRVVGYGSFGFYADAAAAGDTPG
                     DVLIPIARSAIGGIFGLAFRTREDPAFLHAHGACFVTLTRHGKLRGCIGTLQAHRKLL
                     DDVKANAKAAAFLDPRFEPLTATEFKSTLVEISLLSVAEPIAFAGEAEAIAQLRPNLD
                     GIILEFGSNRGTFLPQVWEQIPDPREFLAHLKRKAGLAPDFWADDVRLARYTVSKWKE
                     AELPDPA"
     gene            2922..3644
                     /locus_tag="VD791_12340"
     CDS             2922..3644
                     /locus_tag="VD791_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXV08815.1"
                     /translation="MRTIAAKPSPRWSRRKQARPAELTSAALELFVERGYAATRLEDI
                     ARRAGVSKGTLYLYFDSKEELFKTVVREGLVPALERGERMLDQHRGSAAQLLRELVHG
                     WWELIGNSPFGGIPKLMLSECRNFPELGRFYLEEVISRGYRLLGRVAQRGMDSGEFRR
                     LDLDYVTRLVIAPVVLMAMWRHSFDFCDSHRLDAQRYIDHHLDLLLNGLAHAPSGASN
                     AAANAAVNDGRAWRAGKAGGRA"
     gene            3641..4735
                     /locus_tag="VD791_12345"
     CDS             3641..4735
                     /locus_tag="VD791_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV08816.1"
                     /translation="MKRIFSIARLAAVVSAAAALSACAPKQEHAEEVRPVTTVQVAPA
                     NGEQEVRYTGDVRARYETALGFRVPGKIVVREVEVGSSVKRGQLLARLDPSDFRLNIE
                     AARSQLAAAQADFLQAKDDLARYRNLHEQKFVSAAEFDRRQNAHNVAKARLEQAQAQL
                     GVTQNQSAYTTLRADDDGIITAITAEVGQVVSAGQEVMRLARLEEKEVVISVPESRLG
                     ELDAAREVTITLWAQPDVKFAGRIREISPSADPVTRTYTVKVTLLQPGAEVQLGMTAN
                     VLLGTESAQPIIRLPLTALYQQGEKPAVWIIDPKTSAVVLTPVEVARYTQEAVEISSG
                     LKTGDVVVRAGVHKLTAGQKVRVLAAPDAS"
     gene            4809..7967
                     /locus_tag="VD791_12350"
     CDS             4809..7967
                     /locus_tag="VD791_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV08817.1"
                     /translation="MKGPNLSEWALGHRSFVLFLIVALGCAGVYSYLRLGQAEDPAFT
                     FKVMVVRTLWPGATAREVEQQVTERLEKKIQEVPWFDYTRSYSKPGESMIFVLLKDFT
                     PPPEVPNAWYQARKKIADIRHALPDGVRGPFFNDEFGDTFGTIYAFTADGYSFAELRD
                     YVERVRNELLRVPDVAKIDMIGDQDEKIYVEMSHRKLATLGIDPLQIFNVLRAQNNLV
                     PAGNFETGDDRIFVRVGGAFESPESVREIGIQANNRLFRLGDIATIYRGYVDPPQLKV
                     RYRGQEAITLAIAMRKGGDILALGQALKREMARLTRDLPVGIEVHQVADQPTVVGRSV
                     GEFMKTLSEAVVIVLAVSFLSLGFRTGVVVALSVPLVLAVTFLGMLLAGIDLQRISLG
                     ALIIALGLLVDDAIIAVEMMAIKMEQGWDRFKAASFAYTSTAPSMLTGTLITAAGFLP
                     VGLAKSAAGEYTFSIFAVVGMSLLISWVVAVLFTPYLGFKLLPEAKPGGHTPGLAARL
                     LRKLRPRSAHALAHGADADALAAAAERDVYHTPFYRRVRAVVQWCVDHRFVVIGATVA
                     IFALSLAGFGLVQQQFFPSANRPELVVDLWLPSGASIVATEREAKRFEKVLQADPDVE
                     SYVAYVGGGSPRFYLPLDQQLTNANLVEFVVTTRGNQVRDGVATRLMAKLDNEFTLVR
                     GRVNPLQNGPPVAYPVQFRVSGPDFSELRRIGAQVAEVMRANPNMVHVHLDWNELSKV
                     VKLHIDQNKARLIGVASQDLSNVLNSILTGFSITDYRESDKLIEVLARAEPGERRSLE
                     EIGSINVPTQSGKWIPLTQIATLSYGFEEGLIWRRNREPTITVQGDILGHIQAPVVSN
                     QIDPALEAIRETLPAGYRIEVGGAIEESARGQESVNAVMPVMLLTVVTLLMLHLQSVQ
                     KTVLVLLTAPLGLIGVTLFLLVFQVPFGFVAMLGVIALSGMIMRNSIILVDQIDQDLA
                     GGHAPWDAVVESTVRRFRPIILTAAAAILAMIPLTQSNFWGPMAVAIMGGLLVATALT
                     LLFLPALYAAWFRIRRPTQQPA"
     gene            8089..9786
                     /gene="ggt"
                     /locus_tag="VD791_12355"
     CDS             8089..9786
                     /gene="ggt"
                     /locus_tag="VD791_12355"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXV08818.1"
                     /translation="MLVRAFARPGIALVALLLVVVRVPAAAGTAAIASAHPLATAAGH
                     EILQRGGNAFDAAVAVAAALGVVEPYASGLGGGGFFLLHRAADGKRTMLDARETAPGR
                     ATRTMYLDAEGKPMARLSLDGALAAGIPGTPAALALLARQYGKLPLSAALAPAIRLAR
                     DGFAVDSRYVLAAGFRESLLKTDAAVARIFLDGGRVPKQGYVVRQPELAATLEALAAS
                     GRDGFYAGSVAQRLVEGVKRAGGIWELEDLAGYRVVERQPTTFTYRDARITCAALPSS
                     GGLVLAQALQILERFALPALPRVDRDHLVVEALRRAYQDRARYLGDPDFVALPPQLGT
                     RAYADQRALSIDPAKATSSEALDARYPALRDTAGVREGDNTTHFSIADSQGNLVAATL
                     SVNLPFGAGVLAGDTGVLLNNEMNDFSLAPGAANAYRLTGDAANLVGPGKRPLSSMTP
                     TFVEDGRGVLVLGSPGGSRIISMVLLGILDYVDAPTPDAERLVGAPRFHHQYLPDRVE
                     YEPGAFSPEWIAALQAKGHVVQEGRRRWGNMQAVFVPRATGEASAHGDPRGKVGVLF"
     gene            9851..11080
                     /locus_tag="VD791_12360"
     CDS             9851..11080
                     /locus_tag="VD791_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXV08819.1"
                     /translation="MAIDRRGFLAASAAGAALAGVAPLRTLAADTALVSPALPSGTLD
                     SAQLAALPGKVPLIKRTWRPPNFETPVSWFNQAFTPNDAFFVRYHLANIPEVSSQNWR
                     LAVGGDAAAKSFELTLGQLRSEFEAVEVSALCICSGNRRGLSSPHVGGVQWGYGAMGN
                     ARWKGVRLKDVLARAGVQKEALEVVFDGADGAVLDQTPDFVKSIPVWKAMDDNTLLAY
                     EMNGAPLPHWNGYPLRVVVPGWTATYWMKHLTSIRAVREPERNFWMSTAYRIPKGKFA
                     LVDRFLSQETESNTPITEMVVSSLITNLAQGQRFPAGATVEIRGVAWDGGLGIVAVDV
                     SADGGRSWRPSELGPDLGRFAWRQWRYAIPAARPGSYTVMARATNRVGSSQTYELVFN
                     PAGYHNNVVQRISIQVG"
     gene            11092..11367
                     /locus_tag="VD791_12365"
     CDS             11092..11367
                     /locus_tag="VD791_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXV08820.1"
                     /translation="MKRVVLLAALAAARGLADEGQLHLKEGEGRQLVEANCSMCHSLD
                     YIQMNSPLFDRKGWEASVAKMMKVMGAPIRSEDVPAIVDYLASRYGK"
     gene            11386..12354
                     /locus_tag="VD791_12370"
     CDS             11386..12354
                     /locus_tag="VD791_12370"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HXV08821.1"
                     /translation="MKTTFLEFEQPIAELEAKIDELRYVQEDSALDISEEINRLQKKS
                     HGLTREIYSKLNPWQISQVARHPQRPYTLDYINNIFSDFQELHGDRTFADDASIVGGL
                     ARFAGQSVMVIGHQKGRDTKDKLFRNFGMPRPEGYRKALRLMRLAEKFGVPVMTFVDT
                     PGAYPGVGAEERGQSEAIGRNLYVMAELRVPIIVSVIGEGGSGGALAIAVGDVVLMLQ
                     YATYSVISPEGCASILWKSAERAPDAAESLGITATRLKTLGLIDKVINEPLGGAHRDP
                     NAVFQTLRRALQEALAQVQEKPLAALLEARFNKVMGYGKFKEVSGR"
     gene            12395..13687
                     /gene="tilS"
                     /locus_tag="VD791_12375"
     CDS             12395..13687
                     /gene="tilS"
                     /locus_tag="VD791_12375"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HXV08822.1"
                     /translation="MLEALRRAQLLDGRITLALSGGVDSVVLLDVLVRISRLQPLRLA
                     ALHVNHHISPNASRWAQFCAACCARYGVSCAVADVQVEVAGGESLEAAAREARYAVFQ
                     RQDADALLLAHHLDDQAETLLLQLLRGAGPRGLSGMPQLREAGVRILRPLLEVPRAQI
                     VEYARRERLDWVEDESNALLDFDRNYLRHEVLPRIAARFPAYRETWLRASRNFADASE
                     LADALARIDAESALDGAGLRIDSLRGLGQARARNLLRWFLMQHGVRPPGRERLDEALR
                     QMLGQQPDAQPLVELGPASLRRHRGVARLVQHAEPAAANWQIAWQGEEALPLPHGMGT
                     MCFDSALGAGVSAARLQAAGASVRGRTGGERLKLAADRPSRTLKNLLREAGVPAWQRE
                     GLPLLFCGEAAVWAPGVGCDCRFAAAPGEKGVLPRWVP"
     gene            13776..>14045
                     /locus_tag="VD791_12380"
     CDS             13776..>14045
                     /locus_tag="VD791_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HXV08823.1"
                     /translation="MAVQRTLSIIKPDAVAKNAIGQIYARFEAASLTIVAARMTKLSR
                     EQAEGFYAVHRERSFFRDLVAFMTSGPVMIQVLEGENAIQRNRDLM"
BASE COUNT         2195 a         4678 c         4978 g         2194 t
ORIGIN      
        1 agtctggatg cggcctgacg accaccagct gatgatgcct ctttatcagg tcgtcttcac
       61 ccgcgccggc cagccgggcg tgaagtacga cgccgaggac accgggttcg gctggaagac
      121 cgagggcagg ctcgacgcac gggacgttat caccccgcca acctgcaagg tccagcggcc
      181 ggagcgccaa ggccaataag cgcgccgctg gtaataagta caacggcgcg ttgctctgaa
      241 agcccgcttc tcacgaggcg gcgcgcgggc ttccgtgccc ccgttcttag gccggaagga
      301 aggttgacag ccgtaccggg atgcggcgcc ggccgtgctg gccgcggtac tcctcgaagc
      361 gtccggcgac cggcgcgctg cagcccgcgc agcggccgtc gtgagtcacg cggtactcga
      421 gcagcgtgtg ccagtcacgc acgatcagcg cctcgccgca gccagggcag aacgtggtgc
      481 cgccggtggt gtcgtggacg ttgccggtgt agacgtactg cagcccctcg cggatggcga
      541 tctcgcgcgc gcgcgtgagc gtggctggcg gcgtggccgg caggtcggtc agcttccaat
      601 ccgggtgaaa ggcggtgaag tgcagcggga cgtcggtgcc cagctcccgc gcgatccacc
      661 ggcacatggc cgtgatctcc tcgtcagaat cgttcttcgt cgggatcagc aaggtcgtga
      721 tctcgaacca gacgtcggtc tcgtgcttca agtacaccag cgtgtcgagc accggctgca
      781 gatgcgagcc ggtcagcttg aaatagaact cctcggtgaa cgccttgagg tcgacgttgg
      841 cggcgtccat cttggcgtag aactcgcgcc tcggggcgtc gtgcatgtat ccggcggtga
      901 ctgcgacggt ctggacgccg cgcgcgtggc aggcgtcggc cacatccatg gcgtactcgg
      961 cgaagatcac cgggtcgttg taggtgaacg ccacgctctt gcagccggtg cggtaggccg
     1021 cctcggcgat cagcgccggg ctcgcctggt ccatcaggcg gtccatgtcg cgcgacttgc
     1081 tgatgtccca gttctggcag aacttgcagg cgaggttgca acccgcggtg ccgaacgaca
     1141 ggatgctcga gccggggtag aagtgattga gcggcttctt ctccaccggg tcgatgcaga
     1201 agcccgagga gcgcccgtag gtggtgagca gcatggcgtc gcccacgcgc cggcgcacga
     1261 agcaggcgcc gcgttggccc tcgtgcaacc ggcagtcgcg cgggcacagg tcgcactgca
     1321 tgcgaccgtc gtccagcatg tgccaccagc ggcctggata gttggcttcg ctcatgccgg
     1381 gtccggaagc tccgcctcct tccacttgct gacggtgtag cgcgccaggc ggacgtcgtc
     1441 cgcccagaag tcgggcgcca agccggcttt gcgcttcagg tgcgccagga actcgcgcgg
     1501 gtcggggatc tgctcccaca cctgaggcag gaaagtaccg cggttggaac cgaactcgag
     1561 gatgatgccg tccaggttcg ggcgcagctg cgcgatcgct tccgcttcgc cggcgaaggc
     1621 gatcggctcg gcgaccgaca gcagcgagat ctccaccagc gtggacttga actcggtggc
     1681 ggtgagcggc tcgaagcgcg gatccaggaa cgccgccgcc ttggcgttgg ccttgacgtc
     1741 gtccagcagc ttgcggtgcg cctgcagggt gccgatgcag ccgcgcagct tgccgtggcg
     1801 ggtgagggtg acgaagcagg cgccgtgggc gtgcagaaaa gcagggtcct cgcgcgtgcg
     1861 gaacgccagg ccgaatatcc cgccgatggc cgagcgcgcg atgggaatca gcacatcgcc
     1921 cggcgtatcg ccggcggcgg cagcatcggc atagaagccg aacgatccgt agcccaccac
     1981 gcgcgagcga tcaccggcgg tgtcgcccga gttgcgcagg tcgagcaagc gcgggcccag
     2041 gccgcggcgc gcggccatga gcagcaggcc gttcgccggc gcggcgccac aggcctggtg
     2101 cgggtcgagc gtcggttggc ggttcaggat caggcgcgcg gtgtcgcgat cggtcgcctg
     2161 cgcctgcgcg tagccgagat agtgcgagag gtcggagctc accaccacga gcgtctcgtc
     2221 gccgccccac aggcgctcga gcacctgagc cacctcctcg ggcgcggcgc ggccgaccac
     2281 cagcgggatc agcttgaaat ctccgagcac cttctgcagg aagggcaagt gcacctcgag
     2341 ggagtgctcc agcgcatgcg cttcacggct caccacgact tggtgcaggt cctgcagcag
     2401 cgcgtgccct tcctcgtcca ccgggacgct gccgagcggg gtctcgaacg ccgtcgcgcc
     2461 gggcagggca aggccgcgca cggcgactcg gtgcgcaggg ccgagcagca ccacccgccg
     2521 gatacgcccg gcgaacggca ccagtcgtgc gtacaccgtc gccgcgaccg ggccggagta
     2581 aatgtagccg gcgtggggag cgatgatggc ttttgggacg ggctgcccgg gttcgaggcg
     2641 cgcctgcgcc cgatcgagca gcgcagcgac atcgcgcgcc aagacggcag gatcggccgg
     2701 gtaaaacgcg ccggcgacgg cggcggggcg aatggtggcg gtcatcgatg cagcgatcac
     2761 ccaaaaatga gggcgagccg aagcatttca atagtctaca acttcgaccg aaggcccgac
     2821 cggcttcagt gtgaggcact tcattgacag cctgcggtac cttatatact ttactgacca
     2881 gtcagttatt agcaaacccc aggcatctgg tcagaggagc aatgcgcacc atcgccgcca
     2941 aaccaagccc gcgctggtcc cgccgcaagc aggcgcggcc ggccgagctc acttccgccg
     3001 cgctcgagct gttcgtggag cgcggctacg ccgccacgcg cctggaggac atcgcccggc
     3061 gggcgggcgt cagcaagggc accctgtatc tgtacttcga cagcaaggaa gagctgttca
     3121 agacggtggt gcgtgaagga ctggtgccgg cgctggagcg cggcgagcgc atgctggacc
     3181 agcaccgcgg cagcgcggca cagttgctgc gcgagctggt gcacgggtgg tgggagctga
     3241 tcggcaactc gcccttcggc ggcatcccca agctgatgct gtccgagtgc cgcaactttc
     3301 ccgagctggg gcggttctat ctcgaagagg tgatcagccg cggttaccgg ctgctggggc
     3361 gggtggcgca gcgcggcatg gacagcggcg agttccgcag gctggacctg gactacgtca
     3421 cacggctggt cattgcaccg gtcgtgctga tggcgatgtg gcggcattcc ttcgacttct
     3481 gcgacagcca ccggctcgac gcgcagcgct acatcgatca ccatttggat ctgctgctga
     3541 acgggttggc gcacgcgccc agtggagcga gcaacgcagc ggccaacgcg gcggtcaacg
     3601 acggccgcgc atggcgggcc ggaaaagcgg ggggaagagc atgaaacgga tcttttcgat
     3661 cgcgcgcctg gcggcggtcg tctcggcggc cgcggcgctg tcggcctgcg caccgaagca
     3721 ggagcacgcc gaggaagtgc ggccggtgac cacggtgcag gtcgcccccg cgaacggcga
     3781 gcaggaagtg cgctacaccg gggacgtccg ggcgcggtac gagacggcgc tcggcttccg
     3841 cgtgccgggc aagatcgtgg tgcgcgaggt cgaggtgggt tccagcgtga agcgcggcca
     3901 gctgctcgcg cgactcgacc cgagcgactt ccgcctcaac atcgaagcgg cgcgctcgca
     3961 gctggccgcg gcccaggccg acttcctgca ggccaaggac gatctggcgc gctaccgcaa
     4021 cttgcacgag cagaagttcg tcagcgccgc cgagttcgac cggcgccaga acgcccacaa
     4081 cgtggccaag gcgcgcctgg agcaggcgca agcgcagctc ggcgtgaccc agaaccagtc
     4141 ggcctacacc acgttgcgcg ccgacgacga cgggatcatc actgccatca ccgcggaggt
     4201 gggacaagtg gtgtccgccg gacaagaggt gatgcggctc gcccgcctcg aggagaagga
     4261 agtggtcatc agcgtgccgg agagccgcct gggcgagctg gatgcggcgc gcgaggtgac
     4321 catcacgctg tgggcgcagc cggacgtgaa gttcgccgga cgcattcgcg agatctcgcc
     4381 cagcgccgac ccggtaaccc gcacttacac cgtcaaggtc acgctgctgc agccgggcgc
     4441 ggaggtgcag ctcgggatga cggccaatgt gctgctgggc accgagtccg cgcagccgat
     4501 catccgcctg ccgctgaccg cgctgtacca gcagggcgag aagccggcgg tctggatcat
     4561 cgatccgaag acgagcgcgg tggtgctaac cccggtggag gtggcgcgct acacgcagga
     4621 agcggtggag atcagctccg ggctgaagac cggcgacgtg gtggtgcgcg ccggcgtgca
     4681 caagctcacc gccggccaga aggtgcgcgt gctggccgcg ccggacgctt cgtgacgcgc
     4741 gccgcgccgc gcattccggg gcgcataccg ccgcgcacgc cgacgcgcat gccgacccag
     4801 gccggcgcat gaaaggcccc aatctctcgg aatgggccct cgggcaccgg tccttcgtcc
     4861 tgttcctgat cgtcgcgctc ggttgcgccg gggtctactc ctacctgcgc ctgggccagg
     4921 cggaagaccc ggccttcacc ttcaaggtga tggtggtgcg caccctgtgg ccgggcgcca
     4981 ccgcgcgcga ggtcgagcag caggtcaccg agcggctgga gaagaagatc caggaggtgc
     5041 cctggttcga ctacacgcgc agctactcca agcccggcga gtcgatgatc ttcgtcctgc
     5101 tgaaggactt caccccgccg cccgaggtgc ccaacgcctg gtaccaggcg cgcaagaaaa
     5161 tcgccgacat caggcacgcc ctgcccgacg gcgtgcgcgg cccgttcttc aacgacgagt
     5221 tcggcgacac cttcggcacc atctacgcgt tcaccgcgga cggctattcg ttcgccgagt
     5281 tgcgcgacta cgtcgagcgc gtgcgcaacg agctgctgcg agtgcccgac gtggccaaga
     5341 tcgacatgat cggcgaccag gacgagaaga tctacgtcga gatgtcgcac cgcaagctcg
     5401 ccacgctggg catcgacccc ctgcagatct tcaacgtgct gcgtgcgcag aacaacctgg
     5461 tgcccgccgg caacttcgag accggcgacg accgcatctt cgtgcgcgtg ggcggggcct
     5521 tcgagtcgcc cgagagcgtg cgcgagatcg gcatccaggc caacaaccgc ctgttccggc
     5581 tgggcgacat cgccaccatc taccgcggct acgtggatcc gccgcagctc aaggtgcgct
     5641 accggggcca ggaggcgatc acgctcgcca tcgccatgcg caagggcggc gacatcctgg
     5701 ctctgggcca ggccctgaag cgcgagatgg cgcggctcac gcgcgacctg ccggtgggga
     5761 tcgaagtgca tcaggtcgcc gaccagccca ccgtggtggg gcgttccgtc ggcgagttca
     5821 tgaagacgct gtccgaggcg gtggtcatcg tgctggcggt gagcttcctg agcctggggt
     5881 ttcgcaccgg cgtggtggtc gcgctgtcgg tgccgctggt gctcgccgtc acgtttctgg
     5941 gcatgctgct cgccggcatc gacctgcagc gcatctcctt gggcgcgctg atcatcgccc
     6001 tggggctgct ggtggacgac gccatcatcg cggtggagat gatggcgatc aagatggagc
     6061 agggctggga ccgcttcaag gcggcgagct tcgcctacac ctccaccgcg ccctcgatgc
     6121 tgaccggcac gctgatcacc gccgccggtt tcctgcccgt gggcctcgcc aagtccgcgg
     6181 ccggggagta caccttctcg atcttcgcgg tggtcggcat gtcgctgctg atctcctggg
     6241 tggtggcggt gctgttcacg ccctacctcg ggttcaagct tctgcccgag gcgaagcccg
     6301 gcggccacac ccccggactg gcggcacggc tgctgcggaa attgcggccg cgcagcgcac
     6361 acgcactcgc gcatggcgcg gacgcagacg cgcttgccgc cgcagccgag cgcgacgttt
     6421 atcacacgcc gttctatcgt cgggtacggg cggtggtgca atggtgcgtg gatcaccgtt
     6481 tcgtggtgat cggcgcgacg gtggcgatct tcgccctgtc gctggccggc ttcggcctgg
     6541 tgcagcagca gttctttccg tccgccaacc ggccagagct ggtggtggat ctgtggttgc
     6601 cgagcggtgc atcgatcgtc gccacggaac gcgaagcgaa gcgcttcgag aaggtgctgc
     6661 aggccgatcc ggacgtggag agctacgtgg cctacgtcgg cggaggctcg ccgcgcttct
     6721 acctgccgct cgaccagcaa ctgaccaacg ccaatctggt cgagttcgtc gtcaccacgc
     6781 gcggcaacca ggtgcgcgac ggggtcgcga cgcggctgat ggcgaagctc gacaatgagt
     6841 tcaccctggt gcgcggccgc gtcaatccgc tgcagaacgg cccgccggtg gcctatccgg
     6901 tgcagttccg cgtcagcggc ccggacttct ccgagctgcg ccgcatcggc gcccaggtgg
     6961 cggaggtgat gcgcgccaac ccgaacatgg tgcacgtgca cctcgactgg aacgagctca
     7021 gcaaggtggt gaaactgcac atcgaccaga acaaggcgcg gctcatcggc gtggcgtccc
     7081 aggacctgtc caacgtgctc aattcgattc tcaccggctt cagcatcacc gactaccgcg
     7141 agagcgacaa gctgatcgag gtgctggcgc gcgccgagcc gggcgagcgc cgcagtctgg
     7201 aggaaatcgg cagcatcaac gtgccgaccc agagcggcaa gtggatccct ctgacgcaga
     7261 tcgcgacgtt gagctacggc ttcgaggaag ggctgatctg gcgccgcaac cgcgagccga
     7321 ccatcaccgt gcagggcgac atcctcggcc atatccaggc gccggtggta tcgaatcaga
     7381 tcgacccggc gctggaggcg atccgcgaga cgctgccggc gggctaccgc atcgaggtcg
     7441 gcggcgcgat cgaggagagc gcgcgcggac aggaatcggt caacgcggtg atgccggtca
     7501 tgctgctgac cgtggtcacc ctgctcatgt tgcatctgca gagcgtgcag aagacggtgt
     7561 tggtgctgct gaccgcgcct ctgggcctga tcggcgtcac gctgttcctg ctggtgttcc
     7621 aggtgccgtt cggcttcgtc gccatgctgg gcgtgatcgc tctgtccggc atgatcatgc
     7681 gcaactccat catcctggtc gatcagatcg atcaggacct cgccggcggg catgcgccct
     7741 gggacgcggt ggtcgagtcc acggtgcgcc gcttccgacc catcattctc accgcggcgg
     7801 cggcgatcct ggccatgatc ccgctcaccc aaagcaactt ctggggcccg atggcggtgg
     7861 cgatcatggg cggactgctg gtggccaccg ccctcaccct gctgttcctg ccggcgttgt
     7921 acgcggcctg gttcaggatc cggcggccga cgcagcagcc ggcgtagccc ggatcccgct
     7981 tcgctccatc cgggctacac ggggcgtcgc tgctatcggt cgtggacggg cggtcgtgct
     8041 acattacgcg ccgcttccac cgcaccgctg ccgatgatcc cccgaagctt gctcgtacgc
     8101 gcattcgcgc gtcccggcat cgcgctcgtc gcgctgctgc tcgtcgtcgt gcgcgtcccg
     8161 gccgcggccg ggacggccgc catcgcctcg gcccatccgc tcgccaccgc ggccggacac
     8221 gagatcctgc agcggggcgg caacgccttc gatgcggcgg tggccgtcgc ggcggcgctc
     8281 ggcgtggtcg agccgtatgc ttccggtctg ggcgggggcg gcttcttctt gctgcatcgc
     8341 gccgccgacg gcaagcgcac aatgctcgat gcgcgggaga cggcgcccgg ccgcgccacg
     8401 cgcaccatgt acctggacgc cgaaggaaaa ccgatggcgc ggctgtcgct cgacggagcc
     8461 ctggcggccg gcattccggg cacgccggcc gccttggcgc tgctggcgcg gcaatacgga
     8521 aagctgccgt tgtcggccgc gctcgcgccg gcgatccggc tcgcgcgcga cggcttcgcg
     8581 gtggacagcc gctatgtgct ggcggccggc ttccgcgaga gcctgttgaa gaccgacgcc
     8641 gcggtcgcgc gcatctttct ggacggcggg cgggtgccca agcagggata cgtcgtgcgc
     8701 cagcccgagc tcgccgccac cctggaggcg ctcgcggcca gcggccgcga cggcttctat
     8761 gccggcagcg tggcgcagcg gctggtcgaa ggcgtcaagc gcgccggcgg catctgggag
     8821 ctggaggatc tcgccggcta ccgcgtggtc gagcgccagc ccaccacgtt cacctaccgc
     8881 gatgcgcgca tcacttgcgc cgcgttgccg tcctcgggcg gactggtgct ggcgcaagcg
     8941 ctccagatcc tggagcgttt cgcgctgccc gcgctgccgc gggtggatcg cgaccatctt
     9001 gtggtcgagg cgttgcggcg cgcctatcag gaccgcgcgc gctacctcgg cgatccggac
     9061 ttcgtcgcac tgcccccgca actgggcacg cgcgcctacg ccgaccagcg cgccctgtcc
     9121 atcgacccgg ccaaggccac ttccagcgag gcgctcgatg cccgctaccc ggccttgcgc
     9181 gacactgccg gggtgcgcga gggcgacaac acgacgcact tctccatcgc cgacagccaa
     9241 ggcaatctcg tggcggccac gctcagcgtg aacctgccct tcggcgccgg cgtgctcgcc
     9301 ggggataccg gggtgctgct caacaacgag atgaacgact tctcgcttgc gccgggcgcg
     9361 gccaacgcct accggctcac cggcgatgcg gcgaacctgg tgggccccgg caagcggccg
     9421 ctgtcgagca tgaccccgac cttcgtggag gacggccgcg gcgtgctggt gctgggctcg
     9481 ccgggtggct cgcgcatcat ctccatggta ctgctcggca tcctggatta cgtcgacgcg
     9541 ccgacgccgg acgcggagcg gctggtgggc gcgccgcgtt ttcatcacca gtacctgccg
     9601 gaccgcgtcg agtacgagcc cggggctttc tccccggagt ggatcgccgc gctccaggcc
     9661 aagggccacg tggtgcagga gggccggcgc cgctggggca acatgcaggc cgtgttcgtc
     9721 ccgcgcgcaa ccggcgaggc cagcgcgcac ggcgacccgc gcggcaaggt cggggtgttg
     9781 ttctaggccg cgctcgaccc ggggctttct gctagtgttt tgccgcacca tcggcttgag
     9841 gagatctagc atggccatcg atcgacgcgg ttttctcgcg gcctcggcgg ccggtgcggc
     9901 gctcgccggc gtcgcaccgt tgcgcacgct ggccgccgac acggcgctcg tctcacccgc
     9961 attgccgagc ggcacgctgg attcggccca gctcgcggcg ttgcccggta aggtgccgtt
    10021 gatcaagcgc acgtggcgtc cgccgaattt cgagacgccg gtgagctggt tcaaccaggc
    10081 gttcacaccc aacgacgcat tcttcgtacg ctatcacctc gccaacatcc cggaggtctc
    10141 cagtcagaac tggcgcttgg ccgtgggcgg cgacgcagcc gcgaaatcct tcgagctgac
    10201 gctggggcag ctgcgcagcg agttcgaggc ggtggaggtg agcgccttgt gcatctgctc
    10261 gggcaaccgt cgcgggctgt cctctccgca cgtcggcggc gtccagtggg gctacggtgc
    10321 catgggcaat gcgcgctgga agggcgtgcg gctgaaagac gtgctcgccc gcgccggcgt
    10381 gcagaaagag gcgcttgagg tggtgttcga cggtgccgac ggtgcggtgc tcgaccagac
    10441 gccggacttc gtgaagagca tccccgtctg gaaggcgatg gacgacaaca ccctgctcgc
    10501 ctacgagatg aacggagcac cgctgccgca ctggaatggc tatccgctgc gtgtggtcgt
    10561 cccgggctgg accgcgacct actggatgaa gcacctcacc tcgatccgcg cggtgcgcga
    10621 gcccgagcgc aatttctgga tgtccaccgc ctatcgcatc cccaagggca agttcgccct
    10681 ggtcgaccgc ttcctgtcgc aggagaccga gagcaacacg ccgatcacgg agatggtggt
    10741 gagctcgctg atcaccaatc tggcgcaagg gcagcgcttc cccgccggcg cgacggtcga
    10801 gatccggggc gtggcctggg acgggggctt gggcatcgtc gccgtggacg tgtccgccga
    10861 cggcggccgc agctggcgtc cgtccgagct cggtcccgac ctcgggcgct tcgcctggcg
    10921 ccagtggcgc tacgcgattc cggcggcccg gcccggcagc tacacggtga tggcgcgcgc
    10981 caccaaccgc gtcggttcga gccagaccta cgagttggtg ttcaatcccg ccggctacca
    11041 caacaacgtg gtccagcgta tctccattca agtcgggtga ggaggctgcc gatgaagcgc
    11101 gtagtattgc tggccgcgct ggctgcggcg cgggggttgg ccgacgaggg ccagctgcac
    11161 ctcaaggagg gtgagggcag gcagctggtg gaggccaact gcagcatgtg ccacagcctg
    11221 gattacatcc agatgaattc accgctgttc gaccgcaagg gatgggaggc gtcggtggcg
    11281 aagatgatga aggtgatggg cgcgcccatc cgcagtgaag acgtgcccgc catcgtcgat
    11341 tacctcgcca gccggtacgg caaatagaga cgaggcgggt aagcgatgaa gaccaccttt
    11401 ctggagttcg agcagccgat cgccgagctc gaggccaaga tcgacgagct gcgctatgtg
    11461 caggaagact cggcgctgga catctccgag gagatcaacc ggctgcagaa aaagagtcat
    11521 ggcctgacca gggagatcta ttccaagctc aatccctggc agatctcgca ggtcgcgcgt
    11581 cacccgcagc ggccctacac gctggactac atcaacaaca tcttcagcga cttccaggag
    11641 ctgcacgggg accgtacctt cgccgacgac gcctccatcg tcggcggttt ggcgcgcttc
    11701 gccgggcaga gcgtgatggt gatcggacac cagaagggcc gcgacaccaa ggacaagctg
    11761 ttccgcaact tcggcatgcc gcgccccgag ggctatcgca aggcgctgcg cctgatgcgc
    11821 ctggcggaga agttcggcgt tccggtgatg accttcgtcg acacacccgg cgcttacccg
    11881 ggcgtgggcg cggaggagcg cggccagtcc gaggcgatcg gccgcaacct gtacgtgatg
    11941 gccgagctgc gcgtaccgat catcgtcagc gtgatcggcg agggtggctc cggcggcgcg
    12001 ctcgccatcg ccgtgggcga cgtcgtgctg atgctgcagt acgcgaccta ttcggtgatc
    12061 tcccccgagg gctgcgcctc gatcctttgg aagagcgcgg agcgggcgcc ggacgcggca
    12121 gagagcctgg gcatcacggc cacgcgtctg aagacgctgg gtctgatcga caaggtgatc
    12181 aacgagccgc tgggcggcgc ccaccgcgat cccaacgcgg tgttccagac cctgcgccgc
    12241 gccctgcagg aggcgctcgc gcaggtgcag gagaagccgc tcgcagcgct gctcgaggcg
    12301 cgcttcaaca aggtgatggg ctacggcaag ttcaaggaag tctccggccg ctgagcgggc
    12361 gcgcccgggc ggcggcgatg cgctggtcga tcgcgtgctc gaggcgctgc ggcgcgcaca
    12421 actgctcgac gggcgcatca cgctggcgtt gagcggcggc gtcgactcgg tggtcctgct
    12481 cgacgtgctg gtgcggatct cgcggctgca gccgctgcgc ctggccgccc tgcacgtcaa
    12541 tcatcacatc agcccgaacg cctcgcgctg ggcgcaattc tgcgcggcat gctgcgctcg
    12601 ctacggggtg tcctgcgcgg tggcggacgt gcaggtcgag gtggcgggcg gcgaaagcct
    12661 ggaagccgcg gcgcgcgagg cacgctacgc ggtgttccag cgccaggacg cggacgcgct
    12721 gctgctggcg catcatctgg acgatcaggc cgagaccctc ctgctgcagc tgttgcgcgg
    12781 cgccgggccg cgcgggctga gcggcatgcc gcagctgcgg gaggccggcg tgcgcatcct
    12841 gcgcccgctg ctggaagtgc cgcgcgcgca gatcgtcgag tacgcgcgcc gcgagcgctt
    12901 ggactgggtg gaggacgaga gcaacgcgct gctcgatttc gaccgcaatt acctgcggca
    12961 cgaggtgctg ccgcgcatcg ccgcgcgctt ccccgcttat cgcgagacct ggctgcgcgc
    13021 cagccgcaac ttcgccgatg cgagcgagct ggcggacgcg ctggcgcgca tcgacgccga
    13081 aagcgcgctg gacggcgcgg ggctgcgcat cgattccctg cgcgggctgg ggcaggcccg
    13141 ggcgcgcaac ctgctgcgct ggttcctgat gcagcacggg gtgcggccgc cgggccggga
    13201 acgcctggac gaagcgctgc gccagatgct cgggcagcag ccggacgcgc agccgctggt
    13261 ggagctgggt cctgcgagct tgcgacggca tcgcggcgtg gcgcgcctgg tgcagcacgc
    13321 ggagccggcg gcggcgaact ggcagatcgc ctggcagggc gaagaagcgc tgccgctgcc
    13381 ccacgggatg ggtacgatgt gtttcgacag cgcgctcggc gcgggggtga gcgccgcgcg
    13441 cctgcaggcc gcaggcgcaa gcgtgcgtgg ccgcaccgga ggcgagcggc tcaagctggc
    13501 ggccgaccgt ccttcgcgca cgctgaagaa cctgctgcgc gaagccgggg tgccggcctg
    13561 gcaacgcgag gggttgccgc tacttttctg cggcgaggcc gcggtgtggg cgcccggtgt
    13621 gggctgcgac tgccgcttcg cggcggcgcc gggcgagaag ggcgtgctgc cgcgctgggt
    13681 gccctgagcc cgtgcttcgc cctcttcact tccccaggtt gccgtgctat cgtactgaat
    13741 tctctagaaa agcctttttg ccgggagatg ccgacgtggc agtgcaacgc acgctatcca
    13801 tcatcaagcc ggacgcggtg gcgaagaacg cgatcggaca gatctacgcg cgcttcgaag
    13861 ccgccagcct gaccatcgtg gcggcgcgca tgaccaagct gtcgcgcgag caggccgagg
    13921 gcttctatgc ggtgcatcgc gagcggtcgt tcttccgcga cctggtcgcg ttcatgacct
    13981 cggggccggt gatgatccag gtgctggagg gcgagaacgc gatccagagg aaccgcgatc
    14041 tgatg
//
LOCUS       DASPGE010000381         4351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1153962, whole genome shotgun sequence.
ACCESSION   DASPGE010000381 DASPGE010000000
VERSION     DASPGE010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153962"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /gene="bamC"
                     /locus_tag="VD791_12385"
     CDS             <1..582
                     /gene="bamC"
                     /locus_tag="VD791_12385"
                     /inference="COORDINATES: protein motif:HMM:NF018509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HXV08824.1"
                     /translation="STEVYLTHRGAYEVYVADANVRQSGRTMWQPRQSDPNLEAEMLT
                     RIMVRFGTPETVAAAEVKQAKVEPRATLAKDADGVPVLTLKDDFDRAWRRVGLSLDRL
                     GFSVQDVDRAAGLYYVRYLSPPTPSKDETGFLDRLAFWKKDSNAGEKPEDYRVALAGA
                     DAGTQVTVLSADGKTDKSEAAARMLTVLQEDLR"
     gene            621..1388
                     /locus_tag="VD791_12390"
     CDS             621..1388
                     /locus_tag="VD791_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV08825.1"
                     /translation="MRFAILGSGSQGNCLVVEARNTRLLLDCGFSVGETLRRLGRLGL
                     APEDLSGILVTHEHDDHVGGVARLARKLGLPVWVTPGTLRGLEDLFAGMDGLHLLEGY
                     APLCIGDLEVHPFPVPHDAREPAQYVFGDGARRLGVLTDTGCVTPVMVRMLSGCDALV
                     LESNHDPGLLAAGDYPPALKQRIAGRLGHLDNATAAALLGALDCSRLQHLVAAHLSQS
                     NNRPELAREALAGPLTCAPDWVAIAGQDSGLDWRQIA"
     gene            complement(1473..1673)
                     /locus_tag="VD791_12395"
     CDS             complement(1473..1673)
                     /locus_tag="VD791_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08826.1"
                     /translation="MKHALLIGAFLAVTLAACGKKEEAPPAEPPAPAAEPAPAPEMAP
                     ADQGAASAPEAAPMAPEGEKQE"
     gene            complement(1893..2372)
                     /locus_tag="VD791_12400"
     CDS             complement(1893..2372)
                     /locus_tag="VD791_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08827.1"
                     /translation="MDPHHEKLSGTVEYEAALDRLLARPQRRLCLFDRELGKGFNSAA
                     RAELLRSFLLRDRANRMQVVVHDVSGMARDCPRMITLLRRFPHAIAIHETQPHAKGVY
                     DPFAVADERDHLHRFHYDDARALLVLDDPQATLGFVERFAELWAASEPAVFATTLGL"
     gene            complement(2372..3529)
                     /locus_tag="VD791_12405"
     CDS             complement(2372..3529)
                     /locus_tag="VD791_12405"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV08828.1"
                     /translation="MAGTGADRLLAGMTARTFLARHWQRRPLLVRAAIEGFHGLLLPR
                     DLMRLASRDDVQSRVVLRDDRAWQVHHGPFRPAFFRRLGAQRWTLLVQDVNHFLPQAQ
                     ALLARFRFVPDARLDDLMVSYAPAGGGVGPHFDAYDVFLLQGLGRRRWRVSAQRDLAL
                     VEGAPLQLLARFRATRDWVLAPGDMLYLPPRCAHDGVALDECMTYSIGFRAPAWQELA
                     SAFLAYLQDHLELPGRYADPGLRPARHPARIGADLLRQAQVQLARIRWNRAEVLRFLG
                     SWLTEPKPQVFFRAPKAPPSRAVFLERARRGGLALAPKSRMLYAGGSVFINGECERPA
                     AGALRALQTLADAGGIRLSGREPAHLVELLRRWQRAGWLEFAVRRKQDEEA"
     gene            3648..4115
                     /locus_tag="VD791_12410"
     CDS             3648..4115
                     /locus_tag="VD791_12410"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXV08829.1"
                     /translation="MLKVGERAPDFDLPDCEMELVTLAQFRGAKIVVLFFYPKDGTPG
                     CILQAADFSDLSDRFDALETTVLGVSMDDCLSHGSFRDKHGLSVELLADPEGEVCGKY
                     GVLQEREVEGKKRVGVLRSTFVIDRDGVIRHAMYGVNPRGHAAEVLNLVKGLA"
     gene            4117..>4351
                     /locus_tag="VD791_12415"
     CDS             4117..>4351
                     /locus_tag="VD791_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXV08830.1"
                     /translation="MQIAKNTVVSLTYRLLGGDGKVIERTDEPISYLHGGYRGIFPVV
                     EAALDGKSAGDRCEVRLQPEDAFGDYQSGLVRTE"
BASE COUNT          686 a         1459 c         1545 g          661 t
ORIGIN      
        1 agcaccgagg tgtatctgac ccatcggggc gcctacgagg tctacgtcgc cgacgcgaat
       61 gtgcgccagt ccgggcggac gatgtggcag ccgcgccagt ccgaccccaa cttggaagcg
      121 gagatgctca cccgcatcat ggtccgcttc ggcaccccgg agacggtggc ggccgccgag
      181 gtgaagcagg ccaaagtaga gccgcgcgcc acgctggcca aggatgcgga cggcgtgccg
      241 gtgctcacgc tcaaggacga cttcgaccgc gcctggcggc gcgtggggct gtcgctcgac
      301 cggctgggtt tttcggtaca ggacgtggac cgcgccgcgg gcttgtacta cgtgcgctac
      361 ttgagcccgc ccaccccgtc caaggacgag accggtttcc tcgaccgcct ggcgttctgg
      421 aagaaggatt cgaacgccgg cgaaaagccg gaagactatc gcgtggccct ggcgggtgcc
      481 gatgccggaa cgcaggtcac ggtcctgtcc gcggacggga agaccgacaa gtccgaggca
      541 gccgcgcgca tgttgacggt gctgcaggag gatctgcgat aggcgccgcg ggcgcatcgg
      601 tcgccgccgt ggccgcgtag atgcgcttcg cgatcctcgg cagcggcagc cagggcaact
      661 gcctggtggt cgaggcgcgg aacacgcgcc tgctgctcga ctgcgggttc agcgtcggcg
      721 agacgctgcg gcgactcggc cggctcggtc tcgctcccga ggatctgtcg ggcatcctgg
      781 tcactcacga gcacgacgat cacgtcgggg gggtggcccg actcgcgcgc aagctcgggc
      841 tgccggtgtg ggtgactccc ggtacgctgc gcgggctcga ggacctgttc gcggggatgg
      901 atggtctgca cctcctggag ggttatgccc cgctgtgcat cggcgacctc gaggtgcatc
      961 ccttcccggt gccgcacgat gcgcgcgaac cggcccagta cgtgttcggg gacggcgcgc
     1021 gccgtctggg cgtgctcacc gacaccggtt gcgtgacgcc ggtcatggtg aggatgctga
     1081 gcgggtgcga cgcgctggtg ctggaatcca accacgaccc ggggttgctt gccgcgggcg
     1141 actacccgcc ggcgctgaag cagcgtatcg ccggccgcct cgggcacctg gacaacgcca
     1201 cggccgccgc cctgctcggc gcgctggatt gcagccgcct gcagcacctg gtggcggcgc
     1261 acttgagcca gagcaacaat cgccccgagc tggcgcgcga ggcgctcgcc ggacccctca
     1321 cgtgcgcacc ggactgggtg gcgatcgccg gccaggacag cggtctcgac tggcgccaga
     1381 tcgcctgagc tcccgatcgg gcgggaaggc tcgctcacgc ggaaaagaaa aagccggctc
     1441 tcgggccggc tttttctatg ccgccgagcc ggttactctt gcttttcgcc ttccggcgcc
     1501 atgggcgcgg cttccggagc ggacgcggca ccctggtcgg cgggtgccat ctcaggagcc
     1561 ggagcgggct cggcagccgg agcgggtggt tcggcgggcg gggcttcttc cttcttgccg
     1621 caggccgcga gcgtgactgc caggaaggca ccgatgagaa gcgcgtgttt catctatttt
     1681 tcctcgagga ggtctgagct gttgggggaa cgatcgcttg ccgatgctgc tgccttgtgg
     1741 gttggcgcac ctgccgttgt tacggggcca acttaatcgc gcagtctagc actttcgcgc
     1801 aacaattcca gcgccgttgc ggcgatatac ggatcgcata gcctgtcgtc ggcgggcgtc
     1861 acagcgccat gggcgtcagc cccatggggg cgtcacaaac ccagcgtggt ggcgaacacc
     1921 gccggctcgg aagccgccca cagctctgcg aagcgctcca cgaacccgag cgtggcctgc
     1981 ggatcgtcca ggaccagcag ggcgcgcgcg tcgtcgtagt ggaagcgatg caagtggtcg
     2041 cgctcgtcgg ccacggcgaa agggtcgtat acgcccttgg catgcggttg cgtttcgtgg
     2101 atggcgatcg cgtgcgggaa ccggcgcagc agggtgatca tgcgcgggca atcgcgtgcc
     2161 atgccggaaa cgtcgtgcac caccacttgc atgcggttgg cgcggtcgcg cagcaggaag
     2221 ctgcgcaaca gctcggcgcg cgccgcgctg ttgaagccct tgcccagctc gcggtcgaac
     2281 aggcacagtc tgcgctgcgg gcgcgccagc agccggtcca gggcggcctc gtactcgacg
     2341 gtgccggaca gcttctcgtg atgcgggtcc atcatgcctc ctcgtcctgc ttgcgtctca
     2401 cggcgaactc cagccagccc gcccgctgcc agcgccgcaa cagctccacc aggtgcgcgg
     2461 gctcgcggcc gctgaggcgg atgccgccgg catcggccag ggtctgcaat gcgcgcaacg
     2521 cgcccgccgc gggacgttcg cactcgccgt tgatgaacac gctgccgccc gcgtagagca
     2581 tgcggctttt cggcgccagc gccaaccctc cgcgccgggc gcgctccagg aaaacggcac
     2641 gcgacggcgg cgctttaggc gcgcggaaga acacctgcgg tttcggctcg gtgagccagc
     2701 tccccaggaa gcgcagcact tcggcgcggt tccagcggat gcgcgcgagc tggacttgcg
     2761 cctgccgcag caggtcggcc ccgatgcgcg ccggatggcg ggccggccgc agcccgggat
     2821 ccgcgtaccg ccccggcaac tcgaggtgat cctgcagata ggccaggaat gcgctcgcca
     2881 gctcctgcca cgccggcgcg cgaaagccga tcgagtaggt catgcactcg tccagggcca
     2941 ccccgtcgtg cgcgcagcgc ggcggcaggt acagcatgtc gccgggcgcc agcacccagt
     3001 cgcgcgtggc gcggaagcgc gccagcagct gcagcggcgc gccttccacc agagccagat
     3061 cgcgctgggc gctgacccgc cagcggcgcc gccccagccc ctgcagcaag aacacgtcgt
     3121 aggcgtcgaa atgcgggccg acgccgccgc ccgccggtgc atagctcacc atgagatcgt
     3181 cgaggcgcgc atcgggcacg aagcggaagc gcgcgagcag cgcctgcgcc tgcggcagga
     3241 aatggttcac gtcctgcacc agcagcgtcc agcgctgcgc cccgagacgg cggaagaacg
     3301 ccggccggaa cggtccgtgg tgcacctgcc acgcgcggtc gtcgcgcaac accacgcgcg
     3361 attgcacgtc gtcccgggac gccaggcgca tcaggtcgcg cggcaagagc aggccgtgga
     3421 agccttcgat cgccgcgcgc accagcagcg ggcgtctttg ccagtgccgc gcgagaaagg
     3481 tgcgcgcagt catgcccgcg agcagccggt cggcgccggt gccggccata ggaaaagata
     3541 tcgtgccgtg catggtcgaa aattatagcg gcgcgggcag caggcgccgc gcgacgcttg
     3601 catcaacctg ttagaatcga tcgccccttc gggaagacgc ttggtttatg ctcaaggtag
     3661 gagagcgcgc gccggacttc gacctgccgg attgcgagat ggagctggtg acgctcgcgc
     3721 aattccgcgg cgcgaagatt gtcgtgctct tcttctatcc gaaggacggc acgccgggct
     3781 gcattctgca ggcggccgac ttcagcgatc tgtccgaccg cttcgacgcg ctcgagacga
     3841 cggtgctcgg cgtgagcatg gacgactgcc tgagccacgg ctcctttcgc gacaagcacg
     3901 ggctgtcggt ggagctgctg gccgaccccg aaggcgaggt gtgcggcaag tacggcgtgc
     3961 tgcaggagcg cgaagtggaa ggcaagaagc gggtcggcgt gctgcgttcg accttcgtca
     4021 tcgatcgcga cggcgtgatc cgccacgcca tgtacggggt caacccgcgc gggcacgcgg
     4081 ccgaagtgct gaacctggtg aaagggctgg cctgaattgc agatcgccaa gaacaccgtg
     4141 gtctcgctca cctaccggct gctcggtggc gatggcaagg tcatcgaacg taccgacgaa
     4201 ccgatcagtt atctgcacgg cggctatcgg ggcatcttcc cggtcgtcga agcggcgctc
     4261 gacggcaaga gcgccggcga ccgctgcgag gtgcgcttgc aacccgagga cgccttcggc
     4321 gattaccaat ccggcctggt gcgcaccgag c
//
LOCUS       DASPGE010000382         3631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1154047, whole genome shotgun sequence.
ACCESSION   DASPGE010000382 DASPGE010000000
VERSION     DASPGE010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154047"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VD791_12420"
     CDS             complement(<1..651)
                     /locus_tag="VD791_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020693835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HXV08831.1"
                     /translation="MALNDPQWGRRGNDGPPDLDELWNRFKQKLPGMGGRGGGRGGDA
                     RQFRGGGLILIALVIVVWLASGFYIVDASQRGIVLRFGRHIQTTQPGPQWHLPYPIES
                     VEIVNLSQVRTVEVGYRTNVKNKVLKESLMLTDDENIIDIQFAVQYILKSPEDFLFNN
                     RGTDDAVLQAAETAIREIVGKSKMDFVLYEGREQVAAQATKLMQEILDRYKTGIQIS"
     gene            complement(727..1860)
                     /gene="hflX"
                     /locus_tag="VD791_12425"
     CDS             complement(727..1860)
                     /gene="hflX"
                     /locus_tag="VD791_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HXV08832.1"
                     /translation="MLERPRGGDRAVLVGLDFGERGYAAKFAELHELASSAGLETVAQ
                     VGGKRRRPDPATFAGSGKVTELALRVAEQAATLVVFNHELTPAQERNLEKALGCRVVD
                     RVSLILDIFAQRARSHEGKLQVELAQLERLATRLVRGWTHLERQRGGIGLRGPGETQL
                     ETDRRLLGKRVKVLKEKLAAVQRQREVQRRSRRRAQVLSVALVGYTNAGKSTLFNALT
                     QADAHAADQLFATLDTTTRKLRTPMGGEVVLSDTVGFIRDLPPTLVAAFRATLEETVQ
                     ADLLLHVVDCSGAEQDMQIAEVNRVLADIGAGEVPQILVLNKADLTRLAPGVERDEYG
                     KITRLRVSAKTGAGMDLVRLAVDEFRSGAGRTLHSGSADRAVA"
     gene            complement(2000..2239)
                     /gene="hfq"
                     /locus_tag="VD791_12430"
     CDS             complement(2000..2239)
                     /gene="hfq"
                     /locus_tag="VD791_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HXV08833.1"
                     /translation="MSAKGQLLQDPFLNALRKEHVPVSIYLVNGIKLQGQIESFDQYV
                     VLLKNTVTQMVYKHAISTVVPARAVNLSLDHSSDN"
     gene            complement(2384..>3631)
                     /gene="der"
                     /locus_tag="VD791_12435"
     CDS             complement(2384..>3631)
                     /gene="der"
                     /locus_tag="VD791_12435"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HXV08834.1"
                     /translation="DIPGLTRDRHYGRGRLGNRSFLAVDTGGLEPRARSGIFGAMARQ
                     TLQAVAESDAVLFLVDGREGLTPEDRDIALELRKTGRPLWLVVNKAEGRPREAVAAEF
                     HELGLGDPLPVSAAHGEGVRDLLELVLAPFPEEPVAEDRPAAGPGIAPKIAVVGRPNV
                     GKSTLVNAVLGEERMIASDQPGTTRDSIYVEFRRGGKPYTLIDTAGLRRRGKVQETVE
                     KFSVVKTLQAIEDANVVVLVLDAPQGITDQDAHIAGFILEAGKALVVAVNKWDVADAG
                     RRDAVKREIARKLGFLAFARFHYVSALSGRGLKELLASVDWAYAAAMVRLPTPKLTRT
                     LIEAVNRQPPPRAGLFRPKLRYAHQGGVNPPTIVVHGNQLEQLPDSYRRYLERVFAEA
                     FQLRGTPVRLQFRTGRNPYATRR"
BASE COUNT          508 a         1267 c         1239 g          617 t
ORIGIN      
        1 gctgatctgg atgccggtct tgtagcggtc gagaatctcc tgcatcagct tggtcgcctg
       61 ggccgccacc tgctcgcggc cctcatagag cacgaagtcc atcttgctct tgccgacgat
      121 ctcgcggatc gccgtctccg cggcctgcaa caccgcatcg tcggtcccgc ggttgttgaa
      181 gaggaaatcc tcgggactct tgaggatgta ttgcacggcg aactgaatgt cgatgatgtt
      241 ctcgtcgtcg gtgagcatca gcgattcctt cagcaccttg ttcttgacgt tggtgcggta
      301 acccacctcg acggtgcgca cctggctcag gttgacgatc tccaccgact cgatcggata
      361 gggcagatgc cactgcggcc cgggctgcgt ggtctggatg tgcctgccga agcgcaggac
      421 gatgccgcgc tggctggcgt ccacgatgta gaagccgctc gccagccaga cgacgatcac
      481 caacgcgatc aggatcaagc caccgccacg gaactggcgc gcgtcgccgc cgcggccgcc
      541 gcctctgccg cccatgccgg gcagcttctg cttgaagcgg ttccacagct cgtcgaggtc
      601 gggcgggccg tcgtttccac gccggcccca ctgggggtcg ttcaaggcca tggcaggctg
      661 gctcgagggt gtcctggttg gggagcggtt acgcggctcg cgatgcggcg cgccttgcgt
      721 gaggcctcag gcgaccgcgc ggtcggcaga gcccgaatgg agcgtgcggc cggcgccgga
      781 ccggaactcg tcgacggcca ggcggaccag gtccattcct gcgccggttt tggcgctgac
      841 gcgaaggcgc gtgatcttac catattcgtc gcgctcgacg ccgggcgcaa gccgggtcag
      901 gtcggccttg ttcagcacca ggatctgcgg cacttcgccc gccccgatgt cggccagcac
      961 ccggttcacc tcggcgatct gcatgtcctg ctccgcgccg gaacaatcca ccacatgaag
     1021 caagaggtcc gcctgcacgg tctcctccag ggtggcgcgg aaggccgcca ccagcgtcgg
     1081 gggcaggtcg cggatgaacc cgaccgtgtc cgacagcacc acttcgccgc ccatcggcgt
     1141 gcgcagcttg cgcgtggtgg tgtccagcgt ggcaaacagc tggtcggcgg catgtgcgtc
     1201 ggcctgggtg agcgcattga acagcgtcga cttgccggcg ttggtgtacc ccaccagcgc
     1261 caccgacagc acctgcgcgc ggcgccgcga gcggcgctgc acctcgcgct gacgctgcac
     1321 ggcggcgagc ttctccttga gcaccttgac gcgcttgccg agcaggcgcc ggtcggtttc
     1381 cagctgcgtc tcgccggggc cgcgcaggcc gataccgccg cgctggcgct ccaagtgcgt
     1441 ccagccgcgc accagccgcg tggccaggcg ctcgagctgc gccagctcga cctgcagctt
     1501 gccctcgtgg ctgcgcgcgc gctgcgcgaa gatatccagg atcaggctca cgcggtccac
     1561 cacacggcag ccgagcgcct tctccagatt gcgctcctgc gcgggcgtca gctcgtggtt
     1621 gaacaccacc agggtggcgg cttgctccgc cacgcgcagg gcgagttcgg ttaccttgcc
     1681 gctgccggcg aaggtcgccg ggtcggggcg ccggcgcttg ccgccgacct gcgccaccgt
     1741 ctcgaggccg gcactcgacg cgagctcgtg cagctcggcg aacttggccg cgtagccgcg
     1801 ctcgccgaag tccaggccga cgagaacggc tcggtcgcca ccgcgcgggc gttcgagcat
     1861 ctagcagcgt tccagcgtgt tgtccggggt gcccgatggc cgagcgccgg ggcttcgctt
     1921 cacggcaagg gcaccggcag gctgtgggaa gggtcccggc cggtgccggc cgcgatcgga
     1981 gcggacggat gcgtcccgct cagttgtcag aggagtgatc cagggaaagg ttcacggcgc
     2041 gcgccggcac gaccgtcgag atggcgtgct tgtagaccat ctgcgtgacc gtgttcttga
     2101 gcagcaccac gtactgatcg aacgactcga tctggccctg gagcttgatg ccgttcacca
     2161 gatagatcga tacgggcacg tgctccttgc gcagggcgtt caggaacggg tcttgtagca
     2221 gttgcccctt tgcgctcata gcggcctcta ttgttgtggt ccggtgcgcc tactctactt
     2281 gatttcacag tcgcccgcca tccccgccgg tgcgtcgtac atctcggtca cgcccctcgg
     2341 tcacgcctcc gggcgggcgc ggcaaccggc gggtgccggc ctgtcatctg cgtgtcgcgt
     2401 acgggttgcg ccccgtgcgg aactgcagcc gcaccggcgt gccgcgcagc tggaaggcct
     2461 cggcgaacac ccgttccagg tagcgccggt agctgtccgg aagctgttcc agctggttgc
     2521 cgtggacgac gatggtgggc gggttcacgc cgccctgatg ggcgtagcgc agcttgggcc
     2581 ggaacagccc ggcgcgcggc ggcggctgcc gattgacggc ctcgatcagc gtgcgcgtga
     2641 gcttcggggt gggcaatctg accatggcgg cggcgtaggc ccagtccacc gaagcaagca
     2701 gctccttcag gccgcgcccg ctcagcgccg agacgtagtg gaagcgagcg aaggcgagga
     2761 agccgagctt gcgcgcgatc tctcgcttga ccgcgtcgcg ccggccggcg tcggcgacgt
     2821 cccacttgtt caccgccacc accagcgcct tgcccgcctc gaggatgaag ccggcaatgt
     2881 gcgcgtcctg atcggtgatt ccctgcggcg catccagcac cagcaccacg acgttggcat
     2941 cctcgatcgc ctgcagggtc ttcaccacgg agaacttctc caccgtctcc tgcaccttcc
     3001 cgcggcggcg caggccggcc gtatcgatca gcgtgtaagg cttgccgccc cgccggaact
     3061 cgacgtagat gctgtcgcgg gtggtgcccg gttgatcgga ggcgatcatc cgctcttcgc
     3121 ccagcaccgc gttgaccagc gtggacttgc cgacgttggg cctccccacc actgcgatct
     3181 tgggtgcgat cccggggccg gctgcaggcc ggtcttcggc cactggttct tcggggaacg
     3241 gcgccaggac cagctccagc agatcgcgca cgccttcgcc gtgggcggcc gacaccggca
     3301 gcgggtcgcc cagtcccagc tcgtggaatt cggcggccac cgcttcgcgc gggcggccct
     3361 cggccttgtt gaccaccagc cacagcgggc ggccggtctt gcgcagctcg agcgcgatgt
     3421 cgcggtcttc cggcgtgagt ccctcgcgcc cgtccaccag gaacagcacg gcgtcggact
     3481 cggccaccgc ctgcagggtc tgcctggcca tcgcgccgaa gatgccgctg cgcgcgcgcg
     3541 gctcgagccc gccggtgtcc accgccagga agctgcggtt gccgagccgc ccgcgcccgt
     3601 agtgccggtc gcgcgtgagc ccgggaatgt c
//
LOCUS       DASPGE010000383         4710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_803434, whole genome shotgun sequence.
ACCESSION   DASPGE010000383 DASPGE010000000
VERSION     DASPGE010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803434"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VD791_12440"
     CDS             <1..278
                     /locus_tag="VD791_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HXV08835.1"
                     /translation="TMQLRTLDIYSKAYEELRDKRGIKFVETPRDILEAQLNAWDAVI
                     TEKSAANPFFAKVLESQKAFMQRVVAYQVKFIVPPRQAYEHFFGKLS"
     gene            360..947
                     /locus_tag="VD791_12445"
     CDS             360..947
                     /locus_tag="VD791_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HXV08836.1"
                     /translation="MNNPLFMIDRLSAWAGKAFAWTVVLLTLIICYDVALRYILNRPT
                     QWGFDAAYILYGTLFMMAGAYTLSRNGHVRADMMYRTLRPRTQATVDLVLYFLFFVPG
                     IAALVYAGVEFTRVSWAMKEVSSVTSAGTPIYPFKLVIPIAGGLLLLQGLAEIARCVV
                     CLRTGQWPQRLHDVEEEDIEQLKDILGKDEAGGTR"
     gene            944..2395
                     /locus_tag="VD791_12450"
     CDS             944..2395
                     /locus_tag="VD791_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXV08837.1"
                     /translation="MINALKLVKFEARTIGIGIIAIILLAYILFLPPPAQFTHAHLGM
                     VMLGVIVAAILMGFPIAFTLIGLGILFGIVAFQRPGVPLLENPVFDNMVLRTYGVMTS
                     DVIIAVPLFIFMGYIVERSGIIDRLFRSLELAMSGVPAALAVATIATCAVFAAATGIV
                     GATVTLMGLLVLPPMLKAGYDVKMSAGSIAAGGCLGILIPPSVMLILYGATAGVSVVQ
                     LYMGALIPGIMLSLMYIGYVMIAATINPKLAPKLPAEERNVPAGQIFKMLLTSFFPLA
                     FLIAAVLGSILFGVATPTEAAAVGSLGAAILAASYKRLSFKRLKESAFLTARTTAMVC
                     WLLVGSWVFASTFATLGGQRVLEEWVYSMDLSPGGFLLLSQAIVFLLGWPLDWTVIII
                     IFIPIFLPMLANFGIDPLFFGLLTALNLQTAFLSPPMAMAAYYLKGVAPPQVTLAHIF
                     LGMVPFMLLQLTAVLLLAFFPQIGMWLPGVFYD"
     gene            2636..3634
                     /locus_tag="VD791_12455"
     CDS             2636..3634
                     /locus_tag="VD791_12455"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HXV08838.1"
                     /translation="MKKKVLVTREVFDDTLEYLGQHFEVTSNQANVPYSPQQMTARLQ
                     GMDAAVVTTSDRVDQDFLARGPSLKAVCSVSVGFNHIDLEACTRHGVMATNTPGVLTE
                     SVADMAVCLTLATVRRLTEGERLVRARAWEGTHLKQLLGHDLHHATVGICGLGRIGQT
                     IARRLRGFDTKLVYTDLVRADAQVEAALGAAHLSKDELLRTADIVILILPYTPQTHHY
                     IGQAELDRMKRSAVLINIARGGIVDDSALARALKARRIWAAGLDVYENEPKVDPALFE
                     LDNVVLSPHIASATEPTRQAMAMLAARNCVAALAGEDPPNLLNPEVRSKAQAGGRA"
     gene            3651..>4710
                     /locus_tag="VD791_12460"
     CDS             3651..>4710
                     /locus_tag="VD791_12460"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXV08839.1"
                     /translation="MDELYRLSLVEAARRLASGEMTSEVYARGLLLRAREVDDGIHAW
                     ARLDPEEAIVRAREADARLRAGRTPGPLHGVPVGVKDIINTRGTPTQMGSPIYRGWVP
                     EQNAEVVDRLQTAGAFPLGKTVTTEFAFLTPSKTRNPWNPAHTPGGSSSGSAAAVAAG
                     MVPVAIGTQTNGSVIRPAAFCGVVGYKPGKGVLSTDGILPFSPTLDQPGVFARNVEDA
                     GLLVAHLAHSRWTISPQISALKHAPRLVATRTPVWHLAGEDQRNRFAMDVSGLREAGA
                     VVDEVELPASFNDAHKVHRTIMLYEAAAASRAVRAQHAAQLSELLRRTLEEGDRIAVS
                     DYERALKKHEALQRDFSRF"
BASE COUNT          721 a         1600 c         1584 g          805 t
ORIGIN      
        1 cgaccatgca attgcgcacg ctggacatct actccaaggc ctacgaggag ttgcgcgaca
       61 agcgcggcat caaattcgtg gaaacgccca gggacatcct cgaggcgcag ctcaacgcct
      121 gggatgcggt gatcaccgag aagtcggcgg ccaacccgtt cttcgccaag gtgctggaat
      181 cgcagaaggc gttcatgcag cgcgtggtgg cctatcaggt gaagttcatc gtcccgccgc
      241 gccaggcgta cgagcatttc ttcggcaagc tgagctagcg ccatatcgaa cctccgcgca
      301 ccgcgctcgg ttcctgcacg ggcgcgggtg cggctgcggg aaaaatcggg ggagcaccgg
      361 tgaacaatcc gctgttcatg atcgaccggc tgagcgcctg ggccggcaag gccttcgcgt
      421 ggaccgtcgt gctgctgacg ctgatcatct gctacgacgt ggccctgcgc tacatcctga
      481 accggcccac gcagtggggt ttcgacgccg cctacatcct gtacgggact ttgttcatga
      541 tggccggcgc ctacaccctg tcgcgcaacg ggcacgtgcg tgcggacatg atgtaccgca
      601 cgctcaggcc gcgcacgcag gccaccgtcg acctggtgct ctacttcctg ttcttcgtcc
      661 ccggcatcgc ggcgctggtg tacgccggcg tggagttcac gcgcgtgtcc tgggcgatga
      721 aggaggtgtc cagcgtcacc tcggcgggga cgccgatcta tccgttcaag ctggtgatcc
      781 cgatcgccgg cgggctgctg ctgctgcagg ggctggccga gatcgcgcgc tgcgtggtgt
      841 gcctgcgcac cgggcagtgg ccgcagcgtc tgcacgacgt cgaggaggag gacatcgagc
      901 agctcaagga catcctgggc aaggacgaag ccggaggcac gcgatgatca acgcgctcaa
      961 gctcgtcaag ttcgaggccc gcaccatcgg catcggcatc atcgcgatca tcctgctcgc
     1021 ctacatcctg ttcctgccgc cgcccgcgca gttcacccac gcccacctcg gcatggtgat
     1081 gctcggcgtg atcgtggccg cgatcctgat gggctttccg atcgccttca ccctgatcgg
     1141 gctgggcatc ctgttcggca tcgtcgcctt ccagcgcccg ggggtgccgc tgctggagaa
     1201 ccccgtcttc gacaacatgg tgctgcgcac ctacggggtg atgaccagcg acgtgatcat
     1261 cgcggtgccg ctgttcatct tcatgggcta catcgtcgag cgctcgggca tcatcgaccg
     1321 gctgttccgc agcctggagc tggcgatgtc aggcgtgccc gcggcgctcg ccgtggccac
     1381 catcgccacc tgcgcggtgt tcgctgcggc caccggcatc gtcggcgcga ccgtgacctt
     1441 gatgggcctg ctggtgctgc cgcccatgct caaagccggg tacgacgtga agatgtcggc
     1501 gggttccatc gccgccggcg gctgcctggg catcctgatc ccgcccagcg tcatgctgat
     1561 cctgtacggc gctaccgccg gcgtgtccgt ggtgcagctc tacatgggcg cgctgattcc
     1621 cgggatcatg ctgtccctga tgtacatcgg ctacgtgatg atcgcggcca cgatcaaccc
     1681 caagctcgcg cccaagctgc cggccgagga gcgcaacgtg ccggccgggc agatcttcaa
     1741 gatgctgctg acctcgttct tcccgcttgc gtttctgatc gcggcggtgc tgggcagcat
     1801 tctgttcggc gtggccaccc cgacagaggc cgcggcggtc gggtcgctgg gcgcggccat
     1861 cctggcggcg agctacaagc gcttgagctt caagcgcctc aaggaatcgg cattcctcac
     1921 ggcgcggacc accgccatgg tgtgctggct gttggtcggc tcgtgggtgt tcgcctccac
     1981 cttcgccacg ctgggggggc agcgcgtgct ggaggagtgg gtctattcca tggacctgtc
     2041 gcccggcggc ttcctgctcc tgtctcaggc catcgtgttc ctgctcggct ggccgctcga
     2101 ctggacggtg atcatcatta tcttcatccc gatcttcctg ccgatgctgg ccaacttcgg
     2161 catcgacccg ctgttcttcg ggctgctcac cgcgctcaac ctgcagaccg cgttcctgtc
     2221 gccgccgatg gcgatggcgg cctactacct caagggcgtg gcgccgccgc aggtgacgct
     2281 ggcccacatc ttcctgggga tggttccgtt catgctgctg cagctcacgg ccgtgttgtt
     2341 gctggctttc tttccccaga tcgggatgtg gctgcccggt gtgttctacg actgaagcgg
     2401 gcgttttcta caagttgagc cgcggttgct gccctgaggc ggcgtcttct gcaactgaac
     2461 cagtggctta agcgcgaacc gggcgccctc tgcggctgca gccgggggcg ggcctgggcg
     2521 cttgcctgag cctccccctt caaagtattc tcaacggctt gcagcgggtt cggggacatc
     2581 ggtgtcggtc gtgcggctcc cgaatcgcgc ctacaactcc tataagtccg cgtcaatgaa
     2641 gaagaaagtg ctcgtcacgc gggaggtgtt cgacgacacg ctggagtatc tcggccagca
     2701 tttcgaggtc acctccaacc aggccaacgt cccctacagc ccgcagcaga tgaccgcgcg
     2761 cctgcagggg atggacgcgg cggtcgtcac cacctccgac cgcgtcgacc aggacttcct
     2821 ggcgcgcggc ccgagcctga aggcggtgtg cagcgtgagc gtgggcttca accatatcga
     2881 tctcgaagct tgcacgcggc acggggtcat ggccaccaac accccgggag tgctcaccga
     2941 gagcgtggcc gacatggccg tgtgcctgac gctcgccacc gtgcgccgcc tcaccgaagg
     3001 cgaacggctg gtgcgcgcgc gcgcctggga gggcacgcac ctcaagcagc tgctcggcca
     3061 cgatctgcat cacgccaccg ttggcatctg cggtctgggg cgcatcggcc agaccatcgc
     3121 ccggcggctg cgcggtttcg acaccaagct ggtctatacc gatctcgtgc gcgccgatgc
     3181 gcaggtcgaa gcggcgctcg gcgccgccca tctgagcaag gacgagctgc tgcgcacggc
     3241 cgatatcgtc atcctgatcc tgccttacac gccgcagacg caccactaca tcggccaggc
     3301 cgagctggac aggatgaagc gttcggcggt gctgatcaat atcgcgcgcg gcggcatcgt
     3361 ggacgacagc gcgctcgcgc gcgcgctgaa ggcgcgtcgc atctgggccg ccggcctgga
     3421 cgtctacgag aacgagccca aggtcgaccc ggcgctgttc gagctggaca acgtggtgct
     3481 gtcgccgcac atcgccagcg ccaccgagcc cacgcgccag gcgatggcga tgctcgccgc
     3541 gcgcaactgc gtggcggcgc tcgcggggga agatccgccc aacctcctca acccggaggt
     3601 gaggagcaaa gcccaggcgg gaggccgggc ctaaccataa cccttacaac atggacgaac
     3661 tgtaccgctt gagcctggtg gaggcggcgc ggcggctcgc gtcgggagag atgaccagcg
     3721 aggtgtacgc gcgcgggctg ctgctgcgcg cgcgcgaagt cgacgacggc atccacgcgt
     3781 gggcccgcct cgaccccgag gaggccatcg tgcgcgcgcg tgaagcggac gcgcggctgc
     3841 gcgcggggcg cacgcccggc ccgctgcacg gcgtgccggt cggcgtgaag gacatcatca
     3901 acacccgcgg cacgccgacc cagatgggct cgcccatcta tcggggctgg gtgcccgaac
     3961 agaacgccga ggtggtcgac cggctgcaga cggcgggcgc gtttccgctc ggcaagacgg
     4021 tcaccaccga gttcgcattc ctgacgccct ccaagacacg caacccctgg aatccggcgc
     4081 acacgccggg cggctcgtcc agcggctcgg cggcggccgt ggccgccggc atggtgccgg
     4141 tggcgatcgg cacgcagacc aacggctcgg tcatccggcc ggccgccttc tgcggcgtgg
     4201 tgggctacaa gccgggcaag ggcgtgctgt ccaccgacgg catcctgccg ttcagcccga
     4261 cgctggacca gcccggcgtg ttcgcgcgca acgtggagga tgccgggctg ctggtggcgc
     4321 acctggcgca ttcgcgctgg accatcagcc cgcagatctc ggcgctcaag catgccccgc
     4381 ggctggtcgc cacccgcact ccggtgtggc acctcgccgg cgaggaccag cgcaaccgct
     4441 tcgcgatgga cgtgtcgggg ctgcgcgagg ccggtgcggt ggtggacgaa gtggagctgc
     4501 cggcgagctt caacgacgcg cacaaggtgc atcgcacgat catgctctac gaggccgcgg
     4561 cggcgtcgcg cgcggtgcgc gcgcagcacg ccgcgcagct gagcgagctg ctgcgcagga
     4621 cgctggagga gggcgatcgc atcgccgtct cggactacga gcgcgcgctg aagaagcacg
     4681 aggcgctgca gcgcgacttc tcgcgcttca
//
LOCUS       DASPGE010000384         7101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1154745, whole genome shotgun sequence.
ACCESSION   DASPGE010000384 DASPGE010000000
VERSION     DASPGE010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7101
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154745"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VD791_12465"
     CDS             complement(<1..139)
                     /locus_tag="VD791_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXV08840.1"
                     /translation="MSQSRIRNFSIIAHIDHGKSTLADRFIQMCGGLAEREMAEQVLD
                     TM"
     gene            complement(211..414)
                     /locus_tag="VD791_12470"
                     /pseudo
     CDS             complement(211..414)
                     /locus_tag="VD791_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897649.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            complement(579..1961)
                     /locus_tag="VD791_12475"
     CDS             complement(579..1961)
                     /locus_tag="VD791_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HXV08841.1"
                     /translation="MCRHLLAALALWAPLSLLAQGRDLPDFADLAEKQGPAVVNISTT
                     STARGPQVLPPGAEDDPFFDFFRRFGPPQPREYEARSLGSGFIVSQDGHILTNAHVVE
                     AADEITVRLTDKREYKAKVIGTDKRTDVAVLKIDPTGALPIVAIGDPNKLRVGEWVVA
                     IGSPFGFENTVTAGIVSAKGRSLPQENFVPFLQTDVAINPGNSGGPLFNLKGEVVGIN
                     NMIFSRTGGFMGVSFAIPIDVAIDVAKQLQATGRVSRGRIGVVIQEVTKELAESFGLP
                     KPTGALVNSVEKGGPAEKAGIQASDVILKFDNKEIGSSNELPRIVAATRPGAKVGVQV
                     WRKGERKDLQVTVGETPEERTAKRPQGRKPSGETLGKIGLTVSELSADQRRELNVSGG
                     VLVDSAEGSAARAGIRRGDVILAVGNAEVKSVEELNRLLGGGDKPRTVALLVRRGEGS
                     IYIPLRIDGN"
     gene            complement(2039..2986)
                     /locus_tag="VD791_12480"
     CDS             complement(2039..2986)
                     /locus_tag="VD791_12480"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HXV08842.1"
                     /translation="MISQLWVGAAAAETVEESEALAWLQKIANAARELNYRGTFVYQH
                     GDQTETSRITHFVDRSGEYEKLETLDGPRREIIRNSGEILTFYADTRMVKRERRTARK
                     LFPGLLPDQLAALTAYYQLRKGGQERIAGYDSQQLILQPRDEFRYGHKLWAEVNTGLL
                     LKARMLDDKHQIIEQFHFTEVTINAPLTREAVRPSFPVPAPEARREPGADAAGLADTG
                     WTVRSQPAGFRKIMELWRLKQSGGGQVAHLVYSDGLAAVSVFIEPLPSARQVAEGLSQ
                     QGAVNIYTRPLSDQLVTVLGETPPVTVMQIANSVGPKPR"
     gene            complement(3051..3554)
                     /locus_tag="VD791_12485"
     CDS             complement(3051..3554)
                     /locus_tag="VD791_12485"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HXV08843.1"
                     /translation="MDKISELMDGELPRRESKRQIARLERDPQLSERWDTYHLIRDTL
                     RREVDLGPTFTRRVRACLEHEPVVIAPHTRIGYRMTRYTLPMAAAVAGVAVVAWLALT
                     EAPTGAPTTLAERAAAPAPAMLRAAVNGAASDYLVAHQEFSPRTAMQGVASYARTVTA
                     GEATPGQ"
     gene            complement(3556..4155)
                     /gene="rpoE"
                     /locus_tag="VD791_12490"
     CDS             complement(3556..4155)
                     /gene="rpoE"
                     /locus_tag="VD791_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HXV08844.1"
                     /translation="MSDREIDQQLVERAQRGEKQAFELLVAKYQRKLARLLSRFIRDP
                     AEVEDVAQEAFIKAYRALPSFRGESAFYTWLYRIGINTAKNYLVAMGRRAPTTTEFDS
                     EDAENFEDGEQLRDVNTPENELMSKQIAKTVNETMEALPEELRTAIVLRELDGLSYEE
                     IATVMNCPIGTVRSRIFRARETIAERLRPLLETSKDKRW"
     gene            complement(4299..4760)
                     /locus_tag="VD791_12495"
     CDS             complement(4299..4760)
                     /locus_tag="VD791_12495"
                     /inference="COORDINATES: protein motif:HMM:NF018908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HXV08845.1"
                     /translation="MRLSLGPSSALTWLLSGGHAAAAILVWVTLPPWGSLAACAALAG
                     SLVLALGRHARRSAGAAVVEVALAQDGRVDLRRRDGGRAQGRLLPSTFVSPSLVILNL
                     AGDRWRPARAVLVPADAVGADDHRRLRVWLRWRPVAADKNNDRPGLGRGVN"
     gene            4836..5867
                     /locus_tag="VD791_12500"
     CDS             4836..5867
                     /locus_tag="VD791_12500"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HXV08846.1"
                     /translation="MNTTSSVAAPGRLIEHLRQRGAHPDGAEVAHFGRPDEERAAVGS
                     GATLHALTADAMLEVTGEDARAFLQGQLSNDVDALNGTRAQLSTYCTAQGRMLASLLV
                     WRRADAFLLQLPAELAAPVRSRLQKYVLRAKVKLADISERCALLGLGGAGAGAVLEAA
                     FGAAPREPMARVGFDWGDAICLGSELYEVAVFGDDAAAIWDRLAGLARPTGTRWWRWR
                     LIRAGLPVITGATQELFVPQMANMDALGAVSFDKGCYPGQEIVARAQYRGQVKRRLVR
                     LHAPAGAPQAGQALFAPPAAAAVGTVVNAAPAPAGGVDLLAVVQTDSAGRGALRLGSA
                     DGTPLALAP"
     gene            5878..6168
                     /locus_tag="VD791_12505"
     CDS             5878..6168
                     /locus_tag="VD791_12505"
                     /inference="COORDINATES: protein motif:HMM:NF027614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HXV08847.1"
                     /translation="MPLSYYVYYRVAPERAALARERVGALLAFVARATGVTGRLLSKR
                     DEPHLWMEVYEPVPEGASFGPALARGVRELGIEEVLAPGAQRRQECFEDPCA"
     gene            6159..6938
                     /locus_tag="VD791_12510"
     CDS             6159..6938
                     /locus_tag="VD791_12510"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HXV08848.1"
                     /translation="MCLIVVGIGLRPDVPLMLAANRDEFFARPTQAADFWPDADDVLA
                     GRDLEQGGTWLGLTRGGRLAALTNYREGGRPRTGTRSRGWLVRDFLVSDAEPGPYLGR
                     VQDAAHRFDGFNLLLGGREELHYYSNRGGDPMPLSPGVHGLSNHLLDSPWPKVERAKA
                     RMADLADAPRNALDERLFELLADDRRAPDDELPQTGVSLEWERVLSSAFIRTDAYGTR
                     SSTVILIGADGRMLFEERSFTADGAQAQRRRFELHQEGGGF"
     gene            complement(6948..>7101)
                     /locus_tag="VD791_12515"
     CDS             complement(6948..>7101)
                     /locus_tag="VD791_12515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV08849.1"
                     /translation="EARRLGISEVVLNAQTHALPFYARHGFVAEGPEFMEAGIAHQAM
                     RRRMRP"
BASE COUNT          995 a         2509 c         2486 g         1111 t
ORIGIN      
        1 ccatggtgtc gagcacctgc tcggccatct cgcgctcggc gagcccgccg cacatctgga
       61 tgaagcgatc ggccaaggtc gacttgccgt ggtcgatgtg cgcgatgatg gaaaagttgc
      121 ggatgcggct ctgggacatg agcaaagccg gcgattctat cgcagcttgt ccagcacccc
      181 agggtcgagc cggtagtggc acagctcgtt gccgtcgcgc gcccggtact gcagcgcggc
      241 ggtctgcgcc ttgatggtga tgccgcgctc gcgctccagc tccatcgagt ccagcacctg
      301 gtcggccatc tcccggtcgg agaggccgcc gcaaagctgg atgaaccggt cggccagagt
      361 ggacttgccg tggtcgatgt gcgcgatgat ggaaaagttg cggatgtgct ccatcaggag
      421 gggcgccggc gctcggatcc cggagaatcc gccgcgtcag ataaagccgc gattctagcc
      481 gattttcccg ggcgcgcggg tcggctgcac ggcctctgcg cgagccgtgc acggatcgca
      541 ccggggccgt gccgacgcac ccggcgcggc gcccgggctc agttgccgtc gatgcgcagc
      601 gggatgtaga tcgaaccctc gccgcggcgc accagcaggg ccacggtacg gggcttgtcg
      661 ccgccgccga gcaggcggtt cagctcctcc acggatttca cctcggcgtt gcccaccgcc
      721 aggatgacgt cgccgcggcg gatgccggcg cgcgcggcgc tgccctccgc gctgtcgacc
      781 agcaccccgc cggacacgtt cagctcgcgg cgctgatcgg cgctgagctc cgacaccgtc
      841 aggccgatct tgcccagcgt ctcgccggac ggcttgcggc cctgcgggcg cttggcggtg
      901 cgctcctcgg gggtctcgcc cacggtgacc tgcaggtcct tgcgctcgcc cttgcgccac
      961 acctggacgc cgaccttggc gcccggacgg gtggccgcga cgatgcgcgg cagctcgttg
     1021 gagctgccga tctccttgtt gtcgaacttc aggatcacgt cgctcgcctg gatgccggct
     1081 ttctccgccg ggccgccctt ctccaccgag ttgaccaggg cgccggtggg cttgggcagg
     1141 ccgaaggact cggccagctc cttggtcacc tcctggatca ccacgccgat gcggccgcgg
     1201 ctcacgcggc cggtggcctg cagctgcttg gccacgtcga tcgccacgtc gatggggatg
     1261 gcgaaggaca cacccatgaa gccgccggtg cggctgaaga tcatgttgtt gatgccgacg
     1321 acctcgccct tgaggttgaa cagcggcccg cccgagttgc cgggattgat cgccacgtcg
     1381 gtctgcagga acggcacgaa attctcctgc ggcagcgagc gccccttggc gctgacgatg
     1441 ccggcggtca ccgtgttctc gaagccgaac ggcgagccga tcgccaccac ccattcgccg
     1501 acccgcagct tgttgggatc gccgatggcc acgatcggca gcgccccggt cgggtcgatc
     1561 ttgagcacgg ccacatcggt gcgcttgtcg gtgccgatca ccttggcctt gtactcgcgc
     1621 ttgtcggtga gccgcacggt gatctcgtcg gccgcctcca ccacgtgggc gttggtcagg
     1681 atgtggccgt cctggctgac gatgaagccc gatccgagcg agcgcgcctc gtactcgcgc
     1741 ggctgcggcg gaccgaagcg gcggaagaag tcgaagaacg gatcgtcctc ggccccgggc
     1801 ggcagaacct gcggcccgcg cgcggtcgat gtggtgctga tgttgaccac cgccgggccc
     1861 tgcttctcgg cgaggtcggc gaaatccggc aggtcgcgcc cctgcgcgag caggctgagc
     1921 ggggcccaca gggccagtgc ggccagaaga tgtctgcaca tgctgtgctc gtcccggtga
     1981 tggaaggtgg ggccccgggc tgtgctgcgc cgggggtctg cgccatgcag catgcgtctc
     2041 agcgcggctt ggggcccacc gagttggcga tctgcatgac ggtgaccggc ggcgtctcgc
     2101 ccagcaccgt gaccagctgg tcggacaggg gacgagtata gatgttgacg gcgccctgct
     2161 gtgagaggcc ctcggccacc tggcgcgccg aaggcagcgg ctcgatgaat accgacaccg
     2221 ctgccagccc gtcggagtac accaggtgtg ccacctggcc gccgccgctc tgcttcaggc
     2281 gccacagctc catgatcttg cggaagccgg ccggctggct gcgcacggtc caaccggtgt
     2341 cggccagccc cgcggcgtct gcgccgggct cgcgtcgcgc ttcgggggca gggaccggga
     2401 agctcggcct gaccgcctcg cgcgtgagcg gggcgttgat cgtcacctcg gtgaagtgga
     2461 actgctcgat gatctggtgc ttgtcgtcga gcatgcgcgc cttgagcagc aggccggtgt
     2521 tgacctcggc ccacagcttg tggccatagc ggaactcgtc gcgcggctgc aggatcagct
     2581 gctgcgagtc gtagccggcg atgcgctcct gcccgccctt gcgcagttga tagtacgcgg
     2641 tcagggccgc cagctggtcc gggagcaggc ccgggaacag cttgcgcgcc gtgcgccgct
     2701 cgcgcttgac catgcgcgtg tcggcgtaga aggtcaggat ctcgccgctg ttgcggatga
     2761 tctcgcggcg cgggccgtcg agcgtttcca gcttctcgta ttcgccgctg cggtcgacga
     2821 agtgcgtgat gcgcgaggtc tcggtctgat cgccgtgctg gtagacgaag gtgccgcgat
     2881 agttcagctc gcgggcggcg ttggcgatct tctgcagcca ggcgagcgct tcgctctcct
     2941 ctaccgtctc cgcggcggcc gcgccgaccc atagctggga aatcaccagg gccggcaaca
     3001 ggcccctgct taccgctcgc aacccgggcg ccaccatcat tgaccgggca tcattgaccg
     3061 ggagtggcct cgccggccgt caccgtgcgc gcgtaggagg ccacgccttg catcgcggtg
     3121 cgcggcgaga attcctggtg cgcgaccaga taatcgctcg ccgcgccgtt cacggccgcg
     3181 cgcagcatcg ctggggccgg cgccgccgcg cgctccgcca gggtcgtcgg ggcgcccgtg
     3241 ggcgcctcgg tgagggccag ccacgccacc accgccacgc cggcgacggc ggcggccatc
     3301 ggcagcgtat agcgtgtcat ccgatacccg atcctggtgt gcggcgcgat cacgaccggc
     3361 tcgtgctcca ggcatgcgcg cacgcggcgc gtgaacgtcg gcccgagatc gacctcccgg
     3421 cgcagggtgt cgcggatcag gtggtacgtg tcccagcgct cgctcaactg cgggtcgcgt
     3481 tccagccgcg cgatctggcg tttcgactct cggcgcggca gctcgccgtc catcagttcg
     3541 gagatcttgt ccatctcacc acctcttgtc cttgctcgtc tccagcagcg gccgcagccg
     3601 ctcggcgatc gtttcccgtg cccggaaaat gcgcgagcgc acggtgccga tcgggcagtt
     3661 catcaccgtg gcgatctcct cgtagctcag gccgtccagc tcgcgcagca cgatcgccgt
     3721 gcgcagctcc tccggcagcg cctccatggt ctcgttcacc gttttggcga tctgtttgct
     3781 cattagttca ttttccgggg tgttgacgtc gcgcagctgc tcgccgtcct cgaagttctc
     3841 ggcgtcctcg ctgtcgaact cggtggtggt cggcgcgcgc cggcccatcg cgaccaggta
     3901 gttcttcgcg gtgttgatgc cgatccggta caaccaggtg tagaacgcgc tttccccacg
     3961 gaacgacggc agcgcccggt aggccttgat gaacgcctcc tgggcgacgt cttcgacttc
     4021 ggctgggtcg cggatgaagc gcgacagcag gcgcgcgagc ttgcgctggt atttcgctac
     4081 cagcagctcg aacgcctgtt tttcaccgcg ctgcgcgcgc tcgaccagct gctggtcgat
     4141 ctcacgatcg ctcatcccgg cccggtttca gaggaggctt gtatgccaac aaaaatcgcg
     4201 cgagcgaggt cgcgcgagaa tgtcaagtat accgctaagc cttcccggaa ccgcagccgg
     4261 cggtccctat gccaaagaca aagacccgtc cgataagttc agttcacccc ccgccccaac
     4321 ccgggcctgt cgttgttctt atcggcagca accggccgcc agcgcagcca gacgcgcagc
     4381 cggcgatggt cgtcggcgcc caccgcgtcg gccggcacca ggaccgcgcg cgccgggcgc
     4441 cagcggtctc ccgcgagatt gagaatcacc agcgatggcg agacgaaggt ggacgggagc
     4501 agccgccctt gggcgcgccc gccgtcgcgg cggcgaagat ccacccgccc atcctgggcc
     4561 aacgcgacct ccaccaccgc agcgcccgcc gagcggcgcg cgtggcgacc cagtgcgagc
     4621 accaggctgc cggcgagcgc ggcgcaggca gcgaggctgc cccatggcgg caacgtgacc
     4681 caaaccagta tggcggccgc cgcgtggccg ccgctcagca gccaggtgag cgcgctggac
     4741 gggcccagcg acagcctcaa ggtcgaccca ttcatggcct gaagcaaccc ctataatcgc
     4801 ctctggaccc gtcccgagcg ggcctgcaag cccccatgaa cacgacctcc tccgtggccg
     4861 cacccggccg tctgatcgag catctgcgcc agcgcggcgc gcatccggac ggggccgagg
     4921 tcgcgcactt cggccggccc gacgaggagc gcgcagcggt cggcagcggc gcgacgctcc
     4981 acgcgctcac cgccgacgcg atgctcgagg tcacgggcga ggacgcgcgc gccttcctgc
     5041 agggacagct cagcaacgat gtggacgcgc tgaatgggac gcgcgcgcag cttagcacct
     5101 actgcaccgc gcagggacgc atgctggcca gcctgctcgt gtggcggcgc gccgatgcgt
     5161 tcctgctgca attgcccgcc gagctcgccg ccccggtgcg ctcccgcttg cagaagtacg
     5221 tgctgcgcgc aaaggtaaag ctggcggaca tcagcgagcg ctgcgccctc ctgggcctcg
     5281 gcggggccgg ggccggcgct gtcctcgagg cggcgttcgg cgcggctccc agggagccca
     5341 tggccagggt cggcttcgac tggggcgacg ccatctgcct aggaagcgag ctgtacgagg
     5401 tcgccgtatt cggcgacgat gcggccgcga tttgggaccg cctcgcagga ctggcccgac
     5461 cgacgggcac ccgctggtgg cgctggcgcc tgatccgggc cggtctgccc gtcatcaccg
     5521 gcgcgaccca ggagctgttc gtgccgcaga tggcgaacat ggatgcgctc ggcgcggtga
     5581 gcttcgacaa gggctgctac ccgggccagg agatcgtggc gcgcgcgcag taccgcgggc
     5641 aggtgaagcg gcggctggtg cgcctgcacg cgccggccgg cgcaccgcag gccgggcagg
     5701 cgctgttcgc gccgcccgca gccgccgcgg tgggcaccgt ggtcaacgcc gcgccggcgc
     5761 cggcaggcgg cgtcgacctg ctcgcggtgg tgcaaaccga cagcgccgga cgcggcgcgc
     5821 tgcgcctggg cagcgcagac ggcacgccgc tcgcgctcgc gccgtgacgc ccgccccatg
     5881 ccgctgtcct attacgtcta ttatcgcgtt gcgcccgagc gcgcggcgct cgcgcgcgag
     5941 cgcgtcggcg ccctgctcgc tttcgtcgcg cgcgccacgg gcgtcacggg ccgcctgctg
     6001 agcaagcgcg acgagccgca tctgtggatg gaggtgtacg agccggtgcc ggagggcgcg
     6061 agcttcggcc cggcgctggc gcgcggcgta cgcgagctcg ggatcgagga ggtgctggcg
     6121 cccggcgcgc aacgcaggca ggagtgcttc gaggacccat gtgcctgatc gtcgtcggca
     6181 tcgggctgcg cccagatgtg ccgctgatgc tggccgcgaa ccgcgacgag ttcttcgcac
     6241 gccccacgca ggcggccgac ttctggcccg acgccgacga cgtgctggcc ggccgcgacc
     6301 tcgagcaggg cggcacctgg ctcggcctga cgcgcggcgg ccggctggct gcgctcacca
     6361 actaccgcga gggcggccgg ccgcgcaccg gaacgcgttc gcgcggatgg ctggtgcgcg
     6421 actttctggt gtcggacgcc gaacccggcc cgtacctggg tcgggtgcag gacgccgccc
     6481 accgcttcga cggcttcaac ctgctgctcg gcggccgcga ggagctgcac tactactcca
     6541 accgcggcgg cgaccccatg ccgctgtcgc ccggggtcca cgggctgtcc aatcacctgc
     6601 tcgacagccc ctggccgaag gtggagcgcg ccaaggcgcg catggccgat ctggccgatg
     6661 cgccacgcaa cgcgctcgac gagcgcctgt tcgagctgct cgcggacgac cggcgcgcgc
     6721 ccgacgacga gctgccgcag accggcgtat cgctcgaatg ggagcgcgtg ctgtccagcg
     6781 ccttcatccg caccgacgcg tacggcacgc gctcgtccac ggtgatcctg atcggcgccg
     6841 acgggcggat gctgttcgag gagcgcagct tcaccgccga cggcgcgcag gcgcagcgtc
     6901 gacggttcga gctgcaccaa gaggggggag ggttctgaag tcgcgcctca agggcgcatg
     6961 cggcggcgca tcgcctgatg cgcaatcccc gcctccatga actccggccc ttcggcgacg
     7021 aaaccgtggc gcgcgtagaa gggcagcgca tgggtctgcg cattgagcac cacctccgag
     7081 atcccgaggc gccgcgcttc g
//
LOCUS       DASPGE010000385        13172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1387815, whole genome shotgun sequence.
ACCESSION   DASPGE010000385 DASPGE010000000
VERSION     DASPGE010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13172
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387815"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VD791_12520"
     CDS             <1..290
                     /locus_tag="VD791_12520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HXV08850.1"
                     /translation="VHPIDSLPDWLGTLGRGPAQVRLMLSPAGALRLRELPAPPSVIL
                     LAGPEGGLAPEEQELAELFDFRAVRLGPRILRTETAALAGLAAMHALWGDF"
     gene            290..949
                     /locus_tag="VD791_12525"
     CDS             290..949
                     /locus_tag="VD791_12525"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HXV08851.1"
                     /translation="MKAAAILGALLLAMPVAAHAAKIYKWTDPQGNVIYSDTPRPGAV
                     EIEVPTEPAGIVPVPPRDAAQPSQPAAQPAAYQTLTVVSPADQQVLENIGGLVNVSLA
                     LAPSLQVAEGHAIRLRLDGRTLGTRYPGAEIALPNVERGEHTLAAEVVDRAGAVLIAS
                     AAVSFTVHEPSSEAPAGPDIYPPTVPGQVYPPTYPPVYPPQPYPPQGRPRPDRPRPAP
                     R"
     gene            complement(960..2126)
                     /locus_tag="VD791_12530"
     CDS             complement(960..2126)
                     /locus_tag="VD791_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXV08852.1"
                     /translation="MNQRRDCRITRIDTYVVGAKWTNWVFAHVFTDDGIQGVGEGTCE
                     FQPRAVEAAIQQLARRVAIGQSAFGIERLWQDMFRNEFARGGPILNSAIGAIEMALWD
                     IVGKALDRPIYDLLGGRVRDRLPAYANAWYGAGAGLREIGQAAAAVAAKGYRGLKLDP
                     FADAGRDPDAKLIRAAVDMVACVRDAVGPDLDVLIDCHGRFSPGSAIAIAREMEPSKL
                     YWLEEPCDPENVQALAKIGRSINTRLATGERCYTKYHLQALLAGNEIGVLQPDPIHVG
                     GILEAKKIAAIADAAYTPVSFHNPFGPVATAACVQLDACTTNFILQESFCEYSPAWRF
                     DLVQHAPRPRDGGYDLPSRPGLGVGEFLPEAARAHPFDPDAFLPMWSDAWRSRL"
     gene            complement(2138..2923)
                     /locus_tag="VD791_12535"
     CDS             complement(2138..2923)
                     /locus_tag="VD791_12535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV08853.1"
                     /translation="MPADPAAQVIAVTGGAQGLGEAVCRRMAARGWTVAVADINAEGA
                     ARVAAACARHGASAFPVVVDLASADGPAALVEETLRAAGRLDVLVNCAAVAPAEAFLE
                     MSATAWDAALRVNVRAVALAMAAAGRAMAARGGGRIINITSAAARMALPNYAAYAATK
                     AAVDSVIRTAAVALARHGVRVNSLSPGMMDTPLQMRTEEAFCRLEGRTDLERFREERT
                     ARIPLGRRSTPEEMAQAVEWLAADAPDYMTAERLNVTGGLDKD"
     gene            3048..3812
                     /locus_tag="VD791_12540"
     CDS             3048..3812
                     /locus_tag="VD791_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006483642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HXV08854.1"
                     /translation="MDIRDNTFIVTGGGSGLGGATAEMIVANGGNVIVADISAAGEAF
                     AAKLGRQARFAQTDVSNEQQAKAAVDLALSAFGALRGLVNCAGIAAGEKTVGKEGPHS
                     LASFTRVITVNLIGTFNMIRLAADAMSRQPALASGERGVIVNTASVAAFDGQIGQAAY
                     SASKGGVVGMTLPVARDLARSGIRVVTIAPGIFETPMLKGMPQEVQDSLARQVPFPSR
                     LGKPAEYAALVQHIVGNEMLNGETIRLDGAIRMSPK"
     gene            3836..5155
                     /gene="argA"
                     /locus_tag="VD791_12545"
     CDS             3836..5155
                     /gene="argA"
                     /locus_tag="VD791_12545"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HXV08855.1"
                     /translation="MTEAPEQFVHWFRSAAPYIHAFRGRTFVIAFGGEMVAEGRFQGL
                     THDLNLLASLGVRLVLVHGARPQIEARLAARGSPPRYVRGVRVTDENAMQSVKQASGQ
                     LRVEIEALLSMGLPSSPMANAEIRVAGGNFVTARPRGVLDGVDMQYTGEVRRIHADAI
                     RRRLEAGELVLLSPLGYSPTGEIFNLTVEDLAAEAAIALAADKLLFLTEQPGVGDPDG
                     KLRRELTVSEVEAALRERQDLPEDARLYLPAAVKVCRAGVPRVHLLSRHQDGALLLEL
                     FTHAGVGTMVTRDPLERLREARIDDVGGILALIAPLEADGTLVKRSRELLEMEIGRFA
                     ILEHDAVIVGCAALYPFAQEDAAELACLAVHPDYRNGGAGDRLLRHIESRARAESVQR
                     LFVLTTRAAHWFIERGFAQVEVDVLPKPRQQLYNLQRNSQVFVKALG"
     gene            5355..6170
                     /locus_tag="VD791_12550"
     CDS             5355..6170
                     /locus_tag="VD791_12550"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HXV08856.1"
                     /translation="MVPDAGARAQTPPLEFGLAPYISVRPMMALFEPMAAFLEKRLER
                     PVLLVTAPSLREFDARVLAGRYELAMLAPQAARLAQREAGYVPLLRVSDDLYGVFLVP
                     EESPLRSLRELGDAPIAFPDRFTETAHLGREMVAALGLAPERIVYAPGFQDSLPMSLR
                     RGEHEVALMNESAFHQMKPEQKEGLRVLGETQRIAHVMFLAHPSMPAAQQALIRDAIQ
                     EFWASPEGQRFSAQSGLTGVRPPTENELRTLDPLAREHKRLLDEARKDESEPQ"
     gene            6212..8674
                     /locus_tag="VD791_12555"
     CDS             6212..8674
                     /locus_tag="VD791_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXV08857.1"
                     /translation="MQNPFPRSLRLRLVAATIIVEVVTLTVLVANSLRVNDHALLGQA
                     QTWVSSTTPLLNAALAAPLAQRDYATVLELLEEMRSEHALVYLMVLDHDHSVVAVSGR
                     EASAPLQGPDPSLREAGADSVYDTVVPLTLGGRRYGTLALGVSTAFLQRTQKQLLIQS
                     VLVSSMGVAASLLVLTLLALWIMRHLRRLTSAGAAVAAGDFSARVEVTSEDEVGAVST
                     AFNTMAAAVQDKIRALAEGESRLSEALRELEKANAVQREYLTQAAEERARLNALLAAM
                     QVGILLVDRDNRVIYSNPAFERVWLLARTRVRFLGEDAAAVLAKTTGGAGQPELAELA
                     QAAKEGVPSAPLEIAMSDGRLITRTGYPVRDASEQSIGYLWLFQDVTRERQTANQILY
                     LAERDPLTGLYNRHRFQEELGRMLSGGERRRRRVALLFFDIDEFKHVNDSFGHRAGDA
                     LLIRVAGEVSAQVRRNELFARLGGDEFAILAPDISDQEASVFAERIVRAIGRIPFSFE
                     GNHLRLTCSLGLAMYPDHAATAEDLIAHADAAMYQAKEAGKNTWRVYSEHSSGTSRQM
                     IERLSWNERIEDALEHHLLRLHFQGVFACSDLSLRHLEALVRMVDRSAPDQVIMPGSF
                     IPVAEKTGKILDIDRWVIAASVRTLARHPQVSAIALNISGRSLSEPGLPQFIIEQLKQ
                     QAVQPARLVVELTETSAVSDLHDAQRLIDALRRAGCKVCLDDFGSGFSSFTYLKHLDV
                     DMVKIDGQFIRDLPADRSNQVFVRAMVDVARGLRKATVAESVEDARTLEMLRAFGVDY
                     AQGYHLETPQERHPALLVRETT"
     gene            8767..9042
                     /locus_tag="VD791_12560"
     CDS             8767..9042
                     /locus_tag="VD791_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HXV08858.1"
                     /translation="MPRTVRCVKLGREAEGLDFPPYPGALGQRIHEQVSKEAWQGWLE
                     HQKRLINEMRLDLSNPKSRRYLAEQMEKYFFGSGADQASGYVPPSRA"
     gene            8960..11239
                     /gene="ppk1"
                     /locus_tag="VD791_12565"
     CDS             8960..11239
                     /gene="ppk1"
                     /locus_tag="VD791_12565"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HXV08859.1"
                     /translation="MSRSRWRSTSSARAPTRRPATCPPRARDAAPSYGTTSTAQQARH
                     NKAQPLMGKRSLPQHYLNRELGLLEFNRRVLAQAADAAVPLLERLRFLCIVSSNLDEF
                     FEIRVAGLKAQIEVGADAPGPDGQAPSRVYGEVARGAHELVAAQYRLWNDEVLPSLAK
                     EGIRFFRRRDWDSDQREWIGAYFSREILPVLTPIGLDPAHPFPRILNKSLNFAVQLSG
                     KDAFGRSSGIALVQAPRVLPRVIKLPPKLAGCDYGFVFLSSIIHAHVGELFAGMEVLG
                     CYQFRVTRNSDLFVDEEEVKNLRTALQGELPQRHFGDAVRLEVADLCPGDMSDFLLQQ
                     FGLAPADLYQVNGPVNLVRLMQVPDWVARPDLKYPPFTPGVPSSLVKQPDLFEAIAKG
                     DILLHHPFQSFKPVIAFIAQAAKDPEVAAIKQTVYRTGADSELMRILIDAARRGKEIT
                     VVVELMARFDEEANLNWAAQLEEVGAHVVYGVVGHKTHAKLAMVVRREGERLRRYCHI
                     GTGNYHSRTSTLYTDFGLFTCDEEVCADVNDAFMQLTGLGKAGKLRHLWQSPFTLHSQ
                     LIAAIKREADAAKAGKKVGIIAKMNALLEPEIIDELYEASKAGVRVDLIVRGVCGLRP
                     GISELSENIRVRSIIGRFLEHHRVFHFVSTGNTYLSSADWMDRNFFRRIEVCLPVLDP
                     KLKKRVVEEGLKKYLDDNTQAWEMDSDGHYERCTPGKGEPRNAQLELLQELASAQQPE
                     LDMQAFEKLRPRRKAKAAA"
     gene            11291..12883
                     /locus_tag="VD791_12570"
     CDS             11291..12883
                     /locus_tag="VD791_12570"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HXV08860.1"
                     /translation="MQADGTQGQTLSSPPSAAAATAEVELKLAASPEDAAKFARLPLV
                     KSATTGRAITRHMHSVYYDTPDLALKAQGMALRLRREGTRWVQTLKGAGRVEGGLHQR
                     EEHEVQVPAPLLDHGALAAWGMNGVLADPEQRMRLQPVFTCDFKRTVRHLALSDGTRF
                     ELCTDRGAIAAGEGIDPISEIELELKAGSADRLIDFGLVLLEAVPLRLEPRSKAERGY
                     ALAEQRAEAPTKASAPALRPEMSVTDAFRTVVFACVAHLQANQAGAIAGADIEYLHQA
                     RVALRRLRSAFSVFNRAFPRAALEDLLGELRWLGGQLGPARDCDVFTCTTLPQLASAF
                     RDDAAMQALSERAEALRAAARRDAVEALAAKRYTALLLRLNGTFYRAPWTALDDPPAA
                     ALRAEPLGQFAAEVLARRHRKTLKRGRKHAELDASGLHRLRIEVKKLRYAAEFFSSLY
                     ERKAVRAYTGPLAALQELLGTLNDAATVERLCEVLRKGRDEAAFREALGAMRGWAAAT
                     AHDHLQQLPQAWKRFRDAKPFW"
     gene            complement(12961..>13172)
                     /locus_tag="VD791_12575"
     CDS             complement(12961..>13172)
                     /locus_tag="VD791_12575"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXV08861.1"
                     /translation="IEEVSAFVAAGRGLAIVPWHAVAREIAAHRLRELRAPGWPEVTN
                     NVYAVTRVGTVRSDPAARVLAAIPN"
BASE COUNT         1987 a         4680 c         4598 g         1907 t
ORIGIN      
        1 cggtgcatcc gatcgacagc ctgccggatt ggctgggcac gctcgggcgc ggcccggcac
       61 aggtgcggct catgctgtcg cccgcgggag cgttgcgcct gcgcgagctg ccggcgcccc
      121 cgagcgtgat cctgctggcc ggcccggagg gcgggctggc gcccgaggag caggagctgg
      181 ccgagctgtt cgacttccgc gccgtgcgcc tcggcccgcg catcctgcgc accgaaaccg
      241 ccgcgctggc ggggctcgcc gccatgcatg cgctgtgggg agacttctga tgaaagctgc
      301 tgcaatcctg ggtgccctgc tgctcgcgat gcccgtcgcc gcgcacgcgg cgaagatcta
      361 caagtggacc gacccgcaag gcaacgtgat ctattcggac acgccccgac ccggcgcggt
      421 cgagatcgag gtgccgaccg agccggcggg catcgtgccg gtgcccccac gggacgccgc
      481 acagcccagc cagcccgctg cccagcccgc cgcttaccag acgctgacgg tggtttcacc
      541 cgccgatcag caggttctcg agaacatcgg cggcttggtc aacgtgtcgc tggcgctcgc
      601 cccgtcgctg caggtcgccg aggggcacgc catccgcctg cgtttggacg gacgcacgct
      661 cggcacacgt tacccgggcg ccgagatcgc cctgccgaac gtcgagcgcg gagagcacac
      721 gctggcggcc gaggtggtgg atcgcgcggg cgcggtgctg atcgccagcg cggcggtgag
      781 cttcaccgtg cacgaaccgt cgtccgaagc tccggcgggt ccggacatct acccgcccac
      841 ggttccgggg caagtctatc caccgaccta cccgccggtt tacccgccac agccctatcc
      901 gccgcagggg cgccccaggc ccgatcggcc caggcccgcg ccgcgctgac ccgccgtgct
      961 cagagcctgg accgccaggc gtcgctccac atgggcagga aggcgtcggg atcgaacgga
     1021 tgcgcccgcg ccgcctcggg gagaaactcc cccacgccca gccccggccg cgacggcaga
     1081 tcgtagccgc cgtcgcgcgg ccgcggcgcg tgctgcacca gatcgaaacg ccacgccggc
     1141 gagtactcgc agaacgattc ctgcaggatg aagttggtgg tgcacgcgtc cagctgcacg
     1201 catgcggccg tggccaccgg cccgaaggga ttgtggaagg acaccggcgt gtacgctgcg
     1261 tcggcgatgg cggcgatctt cttcgcctcg agaatgccgc cgacgtggat cgggtcgggc
     1321 tgcagcacgc cgatctcgtt gccggcgagc agcgcctgca gatgatactt ggtgtagcag
     1381 cgctccccgg tcgccagccg cgtgttgatc gagcggccga tcttcgccag cgcctgcacg
     1441 ttctccggat cgcatggctc ctcgagccag tacagcttcg acggttccat ctcgcgcgcg
     1501 atggcgatgg ccgagccggg gctgaagcgg ccgtggcagt cgatcagcac atccaggtca
     1561 ggccccaccg catcgcgcac gcacgccacc atgtccaccg cggcgcggat cagcttcgcg
     1621 tcgggatcgc ggccggcatc ggcgaagggg tcgagcttca gtccgcgata gcccttggcc
     1681 gccaccgccg ccgcggcctg gccgatctcc cgcaggccgg cgccggcgcc gtaccaggcg
     1741 ttggcgtaag ccggcagccg gtcgcgcacg cgcccgccga gcaggtcgta gatcgggcgg
     1801 tcgagcgctt tgccgacgat gtcccacaac gccatctcga tcgctccgat ggccgagttg
     1861 aggatcgggc cgccgcgcgc gaactcgttg cggaacatgt cctgccacag gcgctcgatg
     1921 ccgaaggccg actggcctat cgccacgcgc cgggcgagct gctggatcgc cgcctccacc
     1981 gcgcgcggct ggaactcgca ggtgccctcg cccacgccct ggatgccgtc gtcggtgaac
     2041 acgtgggcga acacccagtt ggtccatttc gcgccgacca cgtaggtgtc gatgcgcgtg
     2101 atgcggcaat cgcggcgctg gttcatcgtg atctcactca gtccttgtcc aatccgccgg
     2161 tcacgttgag ccgctcggcg gtcatgtaat cgggtgcgtc ggcggccagc cactccaccg
     2221 cctgcgccat ctcctcgggc gtgctgcgcc ggcccagcgg gatgcgcgcg gtgcgctcct
     2281 cgcgaaagcg ctcgaggtca gtgcgcccct ccagccggca aaacgcttcc tcggtgcgca
     2341 tctgcagggg cgtgtccatc atgcccggcg agaggctgtt cacccgcacg ccgtgccggg
     2401 cgagcgcgac cgcggcggtg cggatgaccg aatcgacggc tgccttggtg gcggcgtagg
     2461 ccgcgtagtt gggcagcgcc atgcgcgccg ccgccgaggt gatgttgatg atgcgcccgc
     2521 cgccgcgcgc ggccatcgca cgcccggccg cggccatggc gagcgccacg gcgcgcacgt
     2581 tgacacgcaa cgcggcatcc caggcggtcg cgctcatctc cagaaaagcc tccgccggcg
     2641 cgaccgcggc gcagttgacc agcacgtcga ggcggccggc cgcgcgcagg gtttcctcga
     2701 ccagcgccgc cgggccgtcg gcgctggcga gatcgacgac caccggaaaa gcacttgcgc
     2761 cgtgacgcgc gcaggcggcg gcgacgcgcg ccgcaccttc ggcgttgatg tcggccaccg
     2821 ccaccgtcca gccgcgcgcg gccatgcgcc ggcacaccgc ttcgcccagg ccctgggcgc
     2881 cgccggtgac cgcgatgacc tgggcagcgg ggtcggctgg catggccgtc tcagggtggg
     2941 ggaagggtcg ctgtgtgtca agcgcccagc actcgagaaa agcggcgctc gggaaaagcg
     3001 gcagcgtccc ttatacttcg agcttttcgg acgatacagg gaagaacatg gatatccgcg
     3061 acaacacctt catcgtgacc ggcggcggtt cgggcctggg cggggccacg gccgagatga
     3121 tcgtcgccaa cggcggcaac gtgatcgtgg cggacatcag cgcggccggc gaggcgttcg
     3181 ccgcgaagct gggccgtcag gcgcgcttcg cgcaaaccga cgtgtccaac gagcagcagg
     3241 ccaaggcggc ggtggatctc gccctgtccg ccttcggcgc cctgcgcggg ctggtgaact
     3301 gcgccggcat cgccgcgggc gagaagacgg tgggcaagga ggggccgcac tcgctggcga
     3361 gcttcacgcg cgtgatcacg gtcaatctga tcggcacttt caacatgatc cgcctagccg
     3421 ccgacgcgat gtcgcggcag ccggcgctcg cctcgggcga gcgcggcgtg atcgtcaaca
     3481 ccgcctcggt cgccgccttc gacgggcaga tcggacaggc ggcgtattcg gcttccaagg
     3541 gcggggtggt cggaatgacc ttgccggtgg cgcgcgacct cgcgcgcagc ggcatccgcg
     3601 tggtgaccat cgcccccggc atcttcgaga cgccgatgct gaagggcatg ccgcaggagg
     3661 tgcaggactc gctcgccagg caagtgccgt ttccgtcgcg gctgggcaag cccgccgagt
     3721 acgcggcgct ggtgcagcac atcgtgggca acgagatgct caacggcgaa accatccggc
     3781 tggacggcgc gatccggatg agtccgaagt aaccggcacg catggccccg cacgcatgac
     3841 agaggcgccc gagcagttcg tccactggtt ccgttccgcg gcgccctaca tccacgcctt
     3901 ccgcgggcgc accttcgtga tcgctttcgg cggcgagatg gtggccgagg gcaggttcca
     3961 ggggctcacc cacgacctca acctgctcgc cagcctgggc gtgcggctgg tgctggtgca
     4021 cggggcgcgc ccgcagatcg aggcgcggct ggccgcgcgc ggctccccgc cgcgctacgt
     4081 gcgcggcgtg cgcgtgaccg acgagaacgc catgcagagc gtcaagcagg cgagcggcca
     4141 gctgcgcgtg gagatcgaag cgctgctgtc gatgggcctg cccagctcgc cgatggccaa
     4201 cgccgagatc cgcgtggccg gcggcaactt cgtcaccgcg cgcccgcgcg gcgtgctcga
     4261 cggcgtggac atgcagtaca ccggcgaggt gcgcaggatc cacgccgatg cgatccgccg
     4321 ccggctggag gcgggcgagc tggtgctgct gtccccgctc ggctactcgc cgaccggcga
     4381 gatcttcaac ctgacggtgg aggacctcgc cgccgaggcg gcgatcgcgc tcgccgccga
     4441 caagctgctg ttcctcaccg aacagcccgg cgtgggcgat ccagacggga agctgcgacg
     4501 cgagttgacc gtgtccgagg tcgaggccgc gctgcgcgag cggcaggacc tgccggagga
     4561 tgcgcgcctg tacctgccgg ccgcggtcaa ggtgtgccgc gcgggggtgc cgcgcgtgca
     4621 cctgctgtcg cgccaccagg acggcgcgct gctgctggag ctgttcaccc acgcgggcgt
     4681 gggcaccatg gtcacgcgcg atcccctgga gcggctgcgc gaggcgcgca tcgacgacgt
     4741 cggcggcatc ctggcgctga tcgcgccgct ggaagccgac ggcaccctgg tgaagcgctc
     4801 gcgcgagctg ctggaaatgg agatcggccg cttcgcgatc ctggagcacg acgcggtgat
     4861 cgtcggctgc gcggcgctct acccgttcgc gcaggaggac gccgcagagc tggcctgtct
     4921 ggcggtgcat cccgactacc gcaacggcgg cgccggcgac cgcctgctgc ggcacatcga
     4981 atcgcgcgcg cgcgcggagt cggtccagcg gctgttcgtg ctgaccaccc gcgccgcgca
     5041 ctggttcatc gagcgcggct tcgcgcaggt cgaggtggac gtgctgccga agccaaggca
     5101 gcagctgtac aacctccagc gcaactcgca ggtgtttgtc aaagccctgg ggtaggcgcg
     5161 agccgccgcg ccggccgccg gggcggccac gggtacgccc cttgctaaga caaacgctcc
     5221 gacgtgcaag cgctgcgcct gtgacccccc tcgatgctcc cccggatgca tgcagcatga
     5281 ccgcgccggc gcggcacatc cgccctcgga cggcggcttc cgcccccctc gtgtggctcg
     5341 ccgcggtcct ttgcgtggtc cctgacgccg gcgcccgcgc acagacgccg ccgctggagt
     5401 tcgggctggc cccttacatc agcgtgcggc cgatgatggc gctgttcgag ccgatggccg
     5461 cctttctcga gaagcgcctc gagcgcccgg tgctgctggt caccgcgccg tcgctacgcg
     5521 agttcgacgc acgcgtgctg gccggacgct acgagctggc catgctcgcg ccgcaggcgg
     5581 cgcggctggc gcagcgggaa gcgggctacg tgccgctgct gcgcgtgtcc gacgacctgt
     5641 acggggtgtt cctggtgccc gaagagagcc cgctgcgctc gctgcgggag ctgggcgacg
     5701 ccccgatcgc ctttcctgac cgcttcaccg agaccgccca cctgggccgg gaaatggtgg
     5761 ccgcgctcgg actggccccc gagcggatcg tctacgcgcc ggggttccag gattcgctgc
     5821 cgatgtcgct gcgccgcggc gagcacgaag tggcgctgat gaacgagtcg gctttccacc
     5881 agatgaaacc ggagcagaag gagggtctgc gcgtgctggg ggaaacccag cgcatcgcgc
     5941 acgtcatgtt cctggcccac cccagcatgc cggccgcgca gcaggcgctc atccgcgacg
     6001 ccatccagga gttctgggcg agccccgagg ggcagcggtt cagcgcccag agcggcctca
     6061 ccggcgtgcg gccgcccacc gagaacgagc tgcgaacgct ggacccgctg gcgcgcgagc
     6121 acaagcggct gctggacgaa gcgcgcaagg acgagagcga accgcaatga ctctgcgctc
     6181 gcccccgcgt gcacgctgac cgaggctcgc gttgcagaat ccctttcccc gctcgctgcg
     6241 cctgcgcctg gtggcagcca ccatcatcgt ggaagtggtg acgctgacgg tgctggtcgc
     6301 caacagcctg cgggtcaacg accacgcctt gctggggcag gcgcagacct gggtgagcag
     6361 caccacgccg ttgctcaacg ccgcgctcgc cgcgccgctg gcgcagcgcg actacgccac
     6421 cgtgctggag ctgctcgagg agatgcgcag cgagcacgcc ctggtgtatc tgatggtgct
     6481 cgaccacgac cactcggtgg tcgcggtctc ggggcgcgaa gcgagcgccc cgctgcaggg
     6541 ccccgacccg tcgctgcgcg aggcgggtgc cgattcggtc tacgacaccg tggtgccgct
     6601 gaccctgggc gggcgtcgct atggcactct ggccctgggc gtttccaccg ccttcctgca
     6661 gcgcacccag aagcagctgt tgattcaaag cgtgctggtg tcgagcatgg gcgtggcggc
     6721 ctcgctgttg gtgctgacgc tgctggcgct gtggatcatg cggcacctgc ggcggctcac
     6781 tagcgccggc gcggcggtgg cggcgggcga tttctccgcg cgcgtggagg tgacctcgga
     6841 ggacgaggtg ggcgcggtct cgaccgcctt caacaccatg gcggcggcgg tgcaggacaa
     6901 gatccgggcg ctggccgaag gcgagtcacg cctgtccgag gcgctgcgcg agctggagaa
     6961 ggccaatgcg gtgcagcgcg agtatctgac gcaggccgcc gaggaacgcg cgcgcctcaa
     7021 cgcgctgctg gcggcgatgc aggtcggcat cctgctggtc gatcgcgaca accgcgtgat
     7081 ctactccaac cccgcgttcg agcgcgtgtg gctgctcgcg cgcacccgcg tgcgtttcct
     7141 gggggaggac gctgccgcgg tgctggcgaa gaccaccggc ggcgccggcc agccggagct
     7201 ggccgagctg gcgcaggcgg ccaaggaagg ggtgccgtcc gcaccgctcg agatcgcgat
     7261 gagcgacggg cgcctgatca cccgcaccgg ctatccggtg cgcgacgcca gcgagcagtc
     7321 gatcggctac ctgtggctgt tccaggacgt cacccgcgag cggcagaccg caaaccagat
     7381 cctgtacctc gccgagcgcg accccctcac cggcctgtac aaccgccacc gcttccagga
     7441 agagctgggg cgcatgctga gcggcggcga gcgccgccgc cggcgcgtgg cgctgttgtt
     7501 cttcgacatc gacgagttca agcacgtcaa cgacagcttc gggcaccggg ccggcgacgc
     7561 gctgctgatc cgcgtggccg gtgaagtgtc ggcccaggtg cggcgcaacg agctgttcgc
     7621 gcgcctaggc ggcgacgagt tcgcgatcct cgcgcccgac atctcggacc aggaagcgag
     7681 cgtgttcgcc gaacgcatcg tacgcgccat cggccgcatc ccgttcagct tcgagggcaa
     7741 ccacctgcgg ctcacttgca gcctcgggct ggcgatgtac cccgatcacg ccgccacggc
     7801 ggaggacctg atcgcgcatg ccgacgcggc catgtaccag gccaaggagg ccggcaagaa
     7861 cacctggcgc gtctacagcg agcacagctc gggcacctcg cgccagatga tcgagcggct
     7921 gtcctggaac gagcgcatcg aggacgccct cgagcaccat ctgctgcgcc tgcacttcca
     7981 gggcgtgttc gcctgcagcg acctgtcgct gcgccatctg gaggcgctgg tgcgcatggt
     8041 ggaccgctcc gcgccagacc aagtcatcat gcccggcagc ttcatcccgg tggccgagaa
     8101 gaccggcaag atcctggaca tcgaccgctg ggtgatcgcg gccagcgtgc gcacgctggc
     8161 gcggcatccg caggtaagcg ccatcgcgct caacatctcc gggcgctcgc tgtccgagcc
     8221 ggggctgccg cagttcatca tcgagcagct caagcagcag gcggtgcaac ccgcgcgcct
     8281 ggtggtggag ctgaccgaga cctcggcggt gtccgacctg cacgacgccc agcgcctgat
     8341 cgacgcgctg cggcgcgccg gctgcaaggt gtgcctggac gacttcggct cgggcttctc
     8401 ctccttcact tatctcaagc acctggacgt ggacatggtg aagatcgacg gccagttcat
     8461 ccgcgacctg ccggcggacc gcagcaacca ggtgttcgtg cgcgccatgg tggacgtggc
     8521 gcgcgggctg cgcaaggcga cggtggccga atcggtcgag gacgcgcgca cgctggagat
     8581 gctgcgcgcc ttcggcgtgg actacgccca gggctatcat ctggagacgc cgcaggagcg
     8641 gcacccggcg ctgctggtgc gcgagaccac gtagcccgcg ccgcgccgat tcgcgcgata
     8701 atcggcattg cagccgttgc aacgtcccgg taacaatccc tcacccggtt ctaggagaca
     8761 atagccatgc cgcgcaccgt ccgttgcgtc aagctcgggc gcgaggccga ggggctggac
     8821 tttccgccct atcccggtgc gctcggccag cgcatccacg agcaggtctc caaggaggcc
     8881 tggcagggct ggctggagca tcagaagcgc ctgatcaacg agatgcggct cgacctgtcc
     8941 aacccgaagt cgcgccgcta tctcgcggag cagatggaga agtacttctt cggctcgggc
     9001 gccgaccagg cgtccggcta cgtgcccccc tcgcgcgcgt gacgctgcgc ccagttacgg
     9061 cacaacaagc acggcacaac aagcacggca caacaaagca caaccgctca tgggcaagcg
     9121 ttccctcccg cagcactacc tgaaccgcga gctgggcctg ctggagttca accgccgcgt
     9181 gctggcgcag gcggccgatg ccgccgtgcc gctgctcgag cggctgcgct tcctgtgcat
     9241 cgtcagcagc aacctggacg agttcttcga gatccgggtg gccggcctca aggcgcagat
     9301 cgaagtcggc gccgacgcgc ccggccccga cggccaagcg ccctcgcgcg tgtacggcga
     9361 ggtcgccagg ggcgcgcacg agctggtcgc cgcccagtac cggctgtgga acgacgaggt
     9421 cctgccctcg ctcgccaagg agggcatccg cttcttccgg cgacgcgact gggacagcga
     9481 ccagcgcgaa tggatcggcg cctacttctc gcgcgagatc ctgccggtgc tcaccccgat
     9541 cgggctcgac ccggcacatc cgttcccgcg catcctcaac aagagcctca acttcgcggt
     9601 gcagctctcg ggcaaggacg ccttcggccg cagctcgggc atcgcgctgg tgcaagcccc
     9661 gcgcgtgctg ccgcgcgtga tcaagctgcc gcccaagctc gccggctgcg attacgggtt
     9721 cgtgttcctg tcgtcgatca tccacgcgca cgtgggcgag ctgttcgccg gcatggaggt
     9781 gctcggctgc taccagttcc gcgtcacgcg caacagcgac ctgttcgtcg acgaggagga
     9841 ggtgaagaac ctgcgcactg cgctgcaggg cgagctcccg caacgccact tcggcgacgc
     9901 ggtgcgcctg gaggtggccg acctgtgccc cggcgacatg agcgacttcc tgttgcagca
     9961 gttcgggctc gcgcccgcgg acctgtacca ggtgaacggg ccggtcaatc tggtgcgcct
    10021 gatgcaggtc cccgactggg tggcgcgtcc cgatctcaag tatccgccct tcacgcccgg
    10081 cgtgccgtcc agcctggtga agcaacccga cctgttcgag gccatcgcca agggcgacat
    10141 cctgctgcac cacccgttcc agtccttcaa gccggtgatc gccttcatcg cccaggcggc
    10201 caaggacccg gaggtggccg ccatcaagca gacggtgtac cgaaccggcg ccgattcgga
    10261 gctgatgcgc atcctgatcg acgccgcgcg ccgcggcaag gagatcaccg tggtggtgga
    10321 gctgatggcg cgtttcgacg aggaggccaa cctgaactgg gcggcgcagc tcgaggaggt
    10381 gggcgcgcac gtggtctacg gcgtggtcgg gcacaagacc cacgccaagc tcgccatggt
    10441 agtgcgccgc gagggcgagc ggctgcggcg ctactgccac atcggcaccg gcaactacca
    10501 ttcgcgcact tccacgctgt acaccgattt cggcctgttc acctgcgacg aggaggtgtg
    10561 cgccgacgtc aacgacgcct tcatgcagct caccggcctg ggcaaggccg gcaagctgcg
    10621 ccatctgtgg cagtcgccct tcacgctgca ctcgcagctg atcgcggcga tcaagcgcga
    10681 ggccgacgcc gccaaggccg gcaagaaggt gggcatcatc gccaagatga acgccctgct
    10741 cgagcccgag atcatcgacg agctgtacga ggcctccaag gccggggtgc gcgtcgatct
    10801 gatcgtgcgc ggcgtgtgcg gcctgcgccc gggcatctcc gagctgtcgg agaacatccg
    10861 cgtgcgttcc atcatcgggc gcttcctcga gcatcaccgg gtgttccact tcgtgagcac
    10921 cggcaacacc tatctgtcca gcgccgactg gatggatcgc aatttcttcc gccgcatcga
    10981 ggtgtgcctg ccggtcctcg atcccaagct gaagaagcgc gtggtggaag aggggctgaa
    11041 gaagtacctg gacgacaaca cgcaggcctg ggaaatggat tccgacggcc actacgagcg
    11101 ctgcacgccg ggcaagggcg agccgcgcaa cgcccagctc gagctgctgc aagagctcgc
    11161 cagcgcgcag cagccggagc tcgacatgca ggcgttcgag aagctgcggc cgcgccgcaa
    11221 ggccaaggca gcagcctagc gccccctagc cggttttccg acaagacaag gcgccgtctc
    11281 ggccgcgtcc atgcaagccg acggtacgca gggacaaacc ctgtcttccc cgccctccgc
    11341 cgctgccgcg acagcggagg tcgagctcaa gctcgccgcc tcgcccgagg acgccgcgaa
    11401 gtttgcccgc ctgccgctgg tgaagagcgc gaccaccggc cgcgcgatca cccggcacat
    11461 gcacagcgtg tactacgaca cgccggacct ggcgctgaag gcgcaaggca tggcgctgcg
    11521 gctgcggcgc gaaggcacgc gctgggtaca gacgctgaaa ggcgccgggc gcgtggaggg
    11581 cggcttgcac cagcgcgagg aacacgaggt acaggtgccc gcgccgctgc tcgatcacgg
    11641 cgcgctcgca gcttggggga tgaacggcgt gctcgccgat cccgagcagc gcatgcggtt
    11701 gcaacccgtg ttcacctgcg atttcaagcg caccgttcgc cacctcgcgc tgagcgacgg
    11761 cacgcgcttc gagctgtgca cggaccgggg tgcgatcgcc gcgggcgagg ggatcgaccc
    11821 gatcagcgag atcgaactgg agctcaaggc ggggtccgcc gaccggctga tcgacttcgg
    11881 gctggtgctg ctggaggcgg ttcccctgcg gctcgagccg cgcagcaagg ccgagcgcgg
    11941 ctacgccctg gcggagcagc gcgccgaggc gccgaccaag gcgagcgcgc ccgctctgcg
    12001 gccggaaatg agcgtcaccg acgcgttccg caccgtggtg ttcgcctgcg tggcgcattt
    12061 gcaggcgaac caggccggcg cgatcgcggg cgccgacatc gagtatctgc accaggcgcg
    12121 ggtggcgctg cgccggcttc gctccgcgtt cagcgtgttc aaccgcgcct ttccccgcgc
    12181 cgcgctcgag gatctgctgg gcgagctgcg ctggctcggc gggcagctgg gcccggcgcg
    12241 tgattgcgac gtgttcacct gcaccacgct gccccagctc gccagcgcgt ttcgcgacga
    12301 tgccgcgatg caggcgctgt ccgagcgcgc ggaagcgctg cgcgcggccg cgcgccgcga
    12361 tgcggtcgag gcgctcgccg ccaagcgcta caccgcgctg ctgctgcgct tgaacggcac
    12421 cttctaccgc gcgccgtgga ccgcgctgga cgatccgccc gcggccgcgc tgcgcgccga
    12481 accgctcggg cagttcgccg ccgaggtgct cgcgcgccgc caccgcaaga cactgaagcg
    12541 cggacgcaag cacgcggagc tggacgcttc cggcctgcac cgcctgcgca tcgaggtcaa
    12601 gaagctgcgc tacgcggccg agttcttctc ctccctgtac gagcgcaagg cggtgcgcgc
    12661 ttacaccggc ccgctcgccg cgctgcagga gctgctcggc acgttgaacg acgcggccac
    12721 ggtcgagcgc ctgtgcgagg tcctgcgcaa aggtcgcgac gaggccgcct tccgcgaggc
    12781 gctgggcgcc atgcgcggct gggccgccgc caccgcgcac gatcacctgc agcagctgcc
    12841 ccaggcctgg aagcgcttcc gcgacgccaa acccttctgg taggcccttt ctggaccgtt
    12901 ctggtagggc cgttctggta gatccgcccg ccccatgcgc gcggccacgc gcgggaatcc
    12961 tcaattcgga atcgccgcca ggacgcgcgc ggcgggatcc gaacgcaccg tcccgacccg
    13021 cgtcacggcg tacacgttgt tcgtgacttc gggccagccg ggcgcgcgca gctccctcag
    13081 gcggtgcgcg gcgatctccc gtgcgacggc gtgccacggc acgatggcaa gcccgcgccc
    13141 ggcggcgacg aaggcggaca cttcttcgat ct
//
LOCUS       DASPGE010000386         4503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_686799, whole genome shotgun sequence.
ACCESSION   DASPGE010000386 DASPGE010000000
VERSION     DASPGE010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686799"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VD791_12580"
     CDS             complement(<1..498)
                     /locus_tag="VD791_12580"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HXV08862.1"
                     /translation="MRTSAMLAVVALAGLPAQARADVYTCTDAQGRMVFRDTPCPGGE
                     RAGERRDAGAADPGARAQRRGKRAAAGAPLEREQVERLVARFDKAMSKRDAKAVTALL
                     AKDATVQIAGKDGAGREPMQPKAYARYLAEAFARAGYVYEPRTARISLSKSKPRATVT
                     RVVREG"
     gene            complement(687..1856)
                     /locus_tag="VD791_12585"
     CDS             complement(687..1856)
                     /locus_tag="VD791_12585"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HXV08863.1"
                     /translation="MYHPSERHPTLEIMTTGLLTEAFAYGHAAAGHWRAAAEACLEQC
                     GVGARSATLGFVYATDAFADHLAEAVDFLRRRTGIPHWVGTVGLGICASGREYLDEPA
                     MAVMCCAFDDSAFRVLPPVTSPREVAQARVEWGNHSAAFAVLHGDASNRSLTSLVREL
                     AGRTESGFVVGGLTSSRGRSVQYADGASEGGLSGVLFSEDVIVATRLTQGCSPIGPRH
                     RVTETRHNVLIRLDGRPALDVLREDAGRELAANLATAGGHLFAGLPVDGSDTGDYVVR
                     NLVGLDPARKLVAIAQPVGPGAQIVFCRRDRGTASDDLVRMLDSLKTGLYRPPRGALY
                     FSCLGRGASLFGEGSEELRMIADGLGDVPLVGFFCNGEISHNRLYGYTGVLTLFL"
     gene            complement(1889..4138)
                     /locus_tag="VD791_12590"
     CDS             complement(1889..4138)
                     /locus_tag="VD791_12590"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXV08864.1"
                     /translation="MHLLRDALRGAGLLALALGMGPPAQGQEPAAVPEVRARPKIGLV
                     LSGGGARGIAHIGALKVLEELRVPVDYIAGTSMGSIVAGSYALGMSPQEMEKRVLAVD
                     WDRVLADAPPRADRSFRSKALERAGIYGVEAGVSKEGLLLPQGAIAGQNLEVFLAELS
                     GGSVDIGSFDALPIPYRAIATDIETGEAVVLARGNILAAMRSSMSVPGVFSPVEIDGR
                     LLVDGGLVRNLPVDVVRAMGAEVVIAVNLGTPLLKRDEIESVFSVSEQMLNILTEQNV
                     QRSLAELTEADVLIEPELGRYGASDFVNAAKTIPIGEAAARKVADRLRALGLPADEYR
                     ALREAQLARISPTRPVDEVRVDTTGLRHVSPSAVTAEVAEGVKPDLDRVSEDVTSLLA
                     TDDFQQVRYGFIDEDGKRTLVLEPVEKTWGPNYLRFGLNLATDLEGESEFTLIVDHRM
                     TWLNRWGLEWRNTVAVGETTALISELYQPIDPARKFFVAPAVGWIQRSDNLYFEDEAI
                     ARYRTRESQFRLDLGWNVSRAGQVRLGYQVGTVYGKRRIGVLFPDYEVDTGAFLLTAA
                     LDRLDNWAFPSSGYLLTGQYKLSREAVGADLDYERFEVEAQKAFSFGQRRHRLVAGLR
                     YGDSFGSELPLHDLLAAGGFLDLSGLQPRELLVSDRLGVGRLVYYYRWGDPGAFTDLL
                     YLGASLEAASVGDRVNGPDPSGLMPSGSVFVAADTALGPLYLGVGLAESGRYAFYLFL
                     GRPQASFYP"
     gene            4231..>4503
                     /locus_tag="VD791_12595"
     CDS             4231..>4503
                     /locus_tag="VD791_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXV08865.1"
                     /translation="MTTDTAVATQDRLVLRSDAKGVTTLTLNRPQQYNALSEELLAQM
                     ISALEAIEQDPAVRVVVIAGSGPTFCAGHDLKQMRANPSQDYYRKLF"
BASE COUNT          683 a         1649 c         1509 g          662 t
ORIGIN      
        1 gccttcgcgc acgacgcggg tgaccgtcgc gcgcggcttg ctcttcgaga gcgagatgcg
       61 cgccgtcctc ggttcataga catatcccgc gcgcgcgaac gcctcggcca ggtagcgcgc
      121 gtaagccttg ggctgcatcg gctcgcgccc ggctccgtct ttgccggcga tctgcaccgt
      181 ggcgtccttc gcgagcaggg ccgtgaccgc cttggcgtcg cgcttgctca tcgccttatc
      241 gaagcgcgcc acgaggcgct cgacctgctc gcgctccagc ggcgcgccgg ccgctgcgcg
      301 cttgccgcga cgttgcgcgc gggcgcccgg atcggcagcc ccggcgtcgc gccgctcgcc
      361 ggctcgctcg cccccggggc acggcgtgtc gcggaacacc atgcgcccct gcgcatccgt
      421 gcaggtgtag acatcggccc tggcttgcgc cggtagaccg gcgagcgcca cgacggcgag
      481 cattgccgag gttcgcatgc cccctccctg ttttccgacc gcgatgtccg cattcgtgtt
      541 gtctcggcgc gcggactgcg cctcggggga tgttgctcct gcggatcgga aatctcaagc
      601 ccggtggtga cgcgggctct tctttccccg gattgcgctt cgctccatcc gggctacatg
      661 ggatctcgcc gggtcggcga ccggggctag aggaacagcg tgagcacgcc ggtgtagccg
      721 tacagccggt tgtgcgagat ctcgccgttg caaaagaaac ccaccagcgg cacgtcgccc
      781 agcccgtcgg cgatcatgcg cagctcctcc gagccttcgc cgaacaggct cgccccgcgc
      841 cccaggcagg agaagtacag cgccccgcgc ggcggccggt acagcccggt cttgaggctg
      901 tcgagcatgc gcaccagatc gtcgctggcg gtgccgcgat cgcgccgaca gaacacgatc
      961 tgcgcgccgg gtccgaccgg ctgggctatc gccactagct tgcgcgccgg gtcgagcccg
     1021 accaggttgc gcaccacata gtcgcccgtg tcgctgccgt ccaccggcag ccccgcgaac
     1081 agatggccgc cggcggtggc caggttggcg gccagctccc ggccggcgtc ctcgcgcagg
     1141 acgtccaggg ccgggcgccc gtcgaggcgt atcagcacgt tgtgccgcgt ctcggtcacc
     1201 cggtgccgcg ggccgatcgg cgagcacccc tgggtgagcc gggtggcgac gatcacgtcc
     1261 tcggagaaca gcacgccgga cagcccgccc tcgctcgcgc cgtcggcata ttgcacgctg
     1321 cgcccgcgcg agctggtcag cccgcccacc acgaagccgc tctcggtgcg cccggccagc
     1381 tcgcgcacca ggctggtgag cgagcggttg gacgcatcgc cgtgcagcac cgcgaaggcg
     1441 gcgctgtggt tgccccattc cacgcgcgcc tgtgccactt cgcgcggcga ggtcaccggc
     1501 ggcagcaccc ggaacgcgct gtcgtcgaag gcgcagcaca tcaccgccat tgccggctcg
     1561 tccagatact cgcggccgct cgcgcaaatg cccaggccga cggtgccgac ccagtgcggg
     1621 atgccggtgc ggcggcgcag gaaatcgacc gcctcggcca ggtgatcggc gaaggcgtcg
     1681 gtggcgtaga cgaagcccag cgtggcgctg cgcgcgccca cgccgcactg ctccaggcag
     1741 gcctcggcgg ccgcgcgcca gtgccccgcg gccgcgtggc catacgcgaa agcttcggta
     1801 aggaggccgg tggtcatgat ctcgagcgtc gggtggcgct cgctaggatg atacaagacg
     1861 atcgccgcgc gaggaactcg tgactgcttc acggatagaa agaggcctgg ggccgcccga
     1921 ggaacaggta gaacgcatag cgcccgcttt cggccaggcc cacgcccagg tacagcgggc
     1981 cgagcgccgt atcggcggcg acgaacaccg acccggacgg catcaggccc gaaggatccg
     2041 gtccgttcac ccggtcgccg acgctggcgg cctcgagcga ggcgcccagg tagagcaggt
     2101 cggtgaacgc gcccgggtcg ccccaccggt agtaatagac cagccgtccc acgccgagcc
     2161 ggtcgctcac cagcaactcg cgtggctgca gccccgacag gtcgagaaag ccgcccgcgg
     2221 cgagcaggtc gtgcagcggc aactcgctgc cgaacgaatc gccgtaacgc aacccggcga
     2281 ccaagcggtg gcgccgctgg ccgaacgaga acgccttctg ggcttcgact tcgaagcgct
     2341 cgtagtcgag gtctgcgcct acggcttcgc gcgacagctt gtactgcccg gtcaacagat
     2401 aaccggagga aggaaaggcc caattgtcca gccggtcgag cgccgccgtg aggaggaacg
     2461 cgccggtgtc cacctcgtag tcgggaaaca gcacgccgat gcgccgcttg ccgtacacgg
     2521 tgcccacctg gtagcccagg cgcacctgtc cggcgcggct cacgttccac cccaggtcca
     2581 accggaactg cgactcgcgc gtgcgatagc gcgcgatcgc ctcgtcctcg aagtagaggt
     2641 tgtcgctgcg ctggatccag ccgaccgcgg gcgcgacgaa gaacttgcgc gccgggtcga
     2701 tcggctgata cagctcgctg atcaaggccg tggtttcgcc caccgcgacg gtgttgcgcc
     2761 attccagccc ccagcggttg agccaggtca tgcggtgatc gacgatcagg gtgaactcgc
     2821 tctcgccctc caggtcggtg gcgaggttca gaccgaagcg caggtagttc ggtccccagg
     2881 ttttctccac cggctccagc accagcgtgc gcttgccgtc ctcgtcgatg aagccgtagc
     2941 gcacctgctg gaagtcatcg gtggccagca gcgaggtcac gtcttcgctc acgcgatcga
     3001 ggtcgggttt gacgccttcg gcgacctccg cggtcaccgc gctcggactc acgtggcgca
     3061 gcccggtcgt gtccacgcgc acctcgtcca ccgggcgcgt cgggctgatg cgcgccagct
     3121 gggcctcgcg cagcgcccgg tactcgtccg ccggcaggcc gagggcacgc agccggtccg
     3181 cgaccttgcg cgcagccgcc tcgccgatcg ggatggtctt ggccgcgttg acgaagtcgc
     3241 tcgcgccgta gcggcccagc tccggttcga tcaacacatc ggcctcggtc agctcggcga
     3301 gcgagcgctg cacgttctgc tcggtgagga tattgagcat ctgctcgctg accgagaaca
     3361 ccgactcgat ttcgtcgcgc ttcaacagcg gcgttcccag gttgaccgcg atcaccacct
     3421 cggcgcccat cgcgcgcacc acgtccaccg ggagattgcg caccaggccg ccgtccacca
     3481 gcaggcgccc gtcgatctcg acgggagaga acaccccggg caccgacatg ctggagcgca
     3541 tggcggcgag gatgttgccg cgcgccaaca ccaccgcttc gccggtctcg atatcggtgg
     3601 cgatcgcgcg gtaggggatc ggcagcgcgt cgaagctgcc gatgtcgacc gacccgccgc
     3661 tcagctcggc gaggaacact tccaggttct ggccggcgat cgcgccctgc ggcagcagca
     3721 agccctcctt gctcaccccg gcctccaccc cgtagatgcc ggcgcgctcg agcgccttgc
     3781 tgcgaaacga gcgatcggct cgcggcggcg cgtcggcgag cacgcgatcc cagtccacgg
     3841 cgagcacccg cttctccatc tcctgcgggc tcatgcccag cgcgtagctg cccgccacga
     3901 tggagcccat gctggtgccg gcgatgtagt ccaccggcac gcgcagctcc tccagcacct
     3961 tgagggcgcc gatgtgggcg atgccgcgcg cgcccccgcc gctcaacacc agcccgatct
     4021 tcggccgtgc acgcacctcg ggcacggcgg ccggctcctg gccctgcgcc ggcgggccca
     4081 tccccagcgc cagcgccagg agcccggcgc cccgcaaagc gtcgcgcagc agatgcatag
     4141 gtcgtgtcct tttctggaga ggcgccgcgg caggcgggcg cggcggcgcc tataatactg
     4201 tccatcatca tcgacacttc ggccccgaac atgaccaccg acacggctgt cgccacgcaa
     4261 gaccggctgg tgctgcgcag cgatgcgaag ggcgtcacca ccttgacgct gaaccgaccc
     4321 cagcaataca acgcgctgtc cgaggagctg ctggcccaga tgatctcggc gctggaggcg
     4381 atcgagcaag accccgccgt gcgcgtggtg gtcatcgccg gcagcgggcc gactttctgc
     4441 gccgggcacg acctgaagca gatgcgcgcc aatccctcgc aggactacta ccgcaagctg
     4501 ttc
//
LOCUS       DASPGE010000387         2505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_454044, whole genome shotgun sequence.
ACCESSION   DASPGE010000387 DASPGE010000000
VERSION     DASPGE010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454044"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VD791_12600"
     CDS             complement(<1..272)
                     /locus_tag="VD791_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV08866.1"
                     /translation="MTRYFVRQQDVPAYHPANHTGTVNRRLIGAENVGARQLEVVLGV
                     VEKGKGALPHAHPGIEQVCYLLEGEAHVEIGGDKFEMKPGEACFFPP"
     gene            complement(458..838)
                     /locus_tag="VD791_12605"
     CDS             complement(458..838)
                     /locus_tag="VD791_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08867.1"
                     /translation="MLAAWTAVIKYLFPMAYALAEGVPIGSYVFLDFWPVVHVWVGWS
                     LLHWQRYSYALALTVSIAEIVIVVSKFVLFLQAPEWTIWRTNWFINKLFVLACFALLL
                     AYLLLHARQLRAAGPVRAPALVRP"
     gene            complement(936..2138)
                     /locus_tag="VD791_12610"
     CDS             complement(936..2138)
                     /locus_tag="VD791_12610"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXV08868.1"
                     /translation="MIDRRDFLALGAGAALGLAGVTGVQQTAAAGDARIRRYVRLGRT
                     GLEVSDVSFGSSGTSDAALVRYAFERGVNFFDTAESYRGGYAEEAIGEALQGKRDRVV
                     LSSKTKADTGDRRADMMRALEGSLKRLGTDRVDIYFNHAVNDVDRMQNQEWWEFTELA
                     KRQGKIRFRGMSGHGSRLPECLDYAIDHDLVDVVLVAYNFAQDPTFYDRLRHTFHWAA
                     IQPDLPRALKKAKQKNVGVIAMKTLMGARLNDMRPYERRGATFSQAAFRWVLAGEYAD
                     ALVVSMNSTREIDEYLAASGDPKVTTSDLELLERYAQMQTAAYCQSGCRACEGSCPVQ
                     VPIAEVLRTRMYDVDYRRPDLAREEYAALQAPASACLTCAHRSCLQACPRGIPIAEFT
                     RDAALRLG"
     gene            complement(2245..>2505)
                     /locus_tag="VD791_12615"
     CDS             complement(2245..>2505)
                     /locus_tag="VD791_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXV08869.1"
                     /translation="DARIRRYVRLGRIADSQLIPLNELKGRLSEVPRDKPVVAVCHAG
                     MRSGQATVILRGAGFERCANLRGGMLLWSQLGLPTQRAPVSA"
BASE COUNT          422 a          880 c          812 g          391 t
ORIGIN      
        1 ggcgggaaga agcaggcttc gcccggcttc atctcgaact tgtctccgcc aatctccaca
       61 tgcgcctcgc cttccagcag gtagcagacc tgctcgatgc ccggatgcgc gtgcggcagc
      121 gcgcccttgc ctttttccac cacaccgagc accacctcga gttgccgcgc tccgacgttt
      181 tcggccccga tcagccggcg gtttaccgtg ccggtatggt tggccgggtg ataggcgggc
      241 acatcctgct gccgcacgaa gtagcgagtc atgctctccc cctttggtac ctcgctattc
      301 tagcccgggt cattcaggaa gcactgcgca tgctgtcgcc gcacaccaca ccgctccggc
      361 gccgaccgcc ccgtcgcctt cggcgccacc cctccttgcc aggaggggag agctaccccc
      421 tcgtgagagg cggggagcaa gcagtttgaa tcgcgcccta agggcgcacg agcgccgggg
      481 cgcgcaccgg gccggcggcg cgcagttgcc tggcgtggag cagcagatag gcgagcagca
      541 gcgcgaagca ggcgagcacg aacagcttgt tgatgaacca gttggtacgc cagatggtcc
      601 actccggcgc ctgcaggaac aatacgaact tgctcactac gatgacgatc tctgcgatcg
      661 aaacggtgag tgccagcgcg tagctgtagc gctgccagtg cagcagggac cagccgaccc
      721 acacgtgcac caccggccag aagtccagga acacatagct gccgatcggc accccttcgg
      781 ccagcgcgta ggccatcggg aacaggtact tgatgaccgc ggtccaggct gcgagcaaga
      841 acaaaaagtg ggcgaagaaa tgcagcatgg tcaaccgaaa ggcgtgaccg aggggcgtga
      901 ccgaagagcg tgaccggaag gcgtgaccga aagcctcatc cgagcctgag cgcggcatcg
      961 cgggtgaact cggcgatcgg gatcccgcgc gggcaggcct ggaggcagga ccggtgcgcg
     1021 caggtcaggc aggccgacgc cggcgcctgc agcgccgcgt actcctcgcg cgccaggtcc
     1081 ggccggcgat agtccacgtc gtacatgcgc gtgcgcagca cctcggcgat gggcacctgc
     1141 accggacagc tgccttcgca ggcgcggcag ccgctctggc agtaggccgc cgtctgcatc
     1201 tgcgcatagc gctcgagcag ttccaggtcg ctggtggtga ccttcgggtc gccggaggcg
     1261 gccaggtact cgtcgatctc ccgcgtgctg ttcatcgaca ccaccagcgc atcggcatac
     1321 tcgccggcaa gcacccagcg gaaggccgcc tgggagaacg tggcgccgcg ccgctcgtag
     1381 ggccgcatgt cgttcagccg tgcgcccatc agggtcttca tggcgatcac gccgacgttc
     1441 ttctgcttcg ccttcttcag cgcgcgcgga aggtcgggct ggatcgccgc ccagtggaag
     1501 gtgtggcgca ggcggtcgta gaaagtggga tcctgggcga agttgtaggc caccagcacc
     1561 acgtccacca ggtcgtgatc gatggcgtag tccaggcact ccggcagccg gctgccatga
     1621 cccgacatgc cgcggaagcg gatcttgccc tggcgcttgg cgagctcggt aaactcccac
     1681 cattcctggt tctgcatgcg gtccacgtcg ttgaccgcgt ggttgaagta gatgtccacg
     1741 cgatcggtgc ccaagcgctt gaggctgccc tccagcgcac gcatcatgtc cgcgcggcga
     1801 tcgcccgtgt ccgccttggt cttgctggac agcaccaccc gatcgcgttt gccctgcaac
     1861 gcctcgccga tcgcctcttc cgcatagcca ccgcggtagc tctccgccgt atcgaagaag
     1921 ttcacgccac gctcgaaggc gtagcgcacc agcgccgcat cggaggtgcc ggaggagccg
     1981 aaggagacgt cggagacctc gagcccggtg cggcccagcc gcacgtaacg gcggatgcgc
     2041 gcgtcgccgg ccgctgcggt ctgctgcacg ccggtcacgc ccgccaggcc cagcgcagcg
     2101 ccggcgccca gcgccaggaa gtcgcgtcta tcgatcatgc ctgatcactc ctgtcgtcgt
     2161 tgcgccgcca tcgggtcggg gatgtcggct cacaccatgc cgacacccta cgcctgtgcc
     2221 gacccctacg ccgacgccct atgcctatgc cgacaccggg gcgcgctgcg tgggcagccc
     2281 gagctgcgac cacagcagca tgcccccgcg caggttggcg cagcgctcga agccggcccc
     2341 gcgcaggatc acggtcgcct gtcccgaacg catcccggcg tggcacaccg cgaccaccgg
     2401 cttgtccctc ggcacttccg acagccgccc cttcaactcg ttcaacggga tgagctggct
     2461 gtcggcgatg cggcccagcc gcacgtaacg gcggatgcgc gcgtc
//
LOCUS       DASPGE010000388         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1037954, whole genome shotgun sequence.
ACCESSION   DASPGE010000388 DASPGE010000000
VERSION     DASPGE010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037954"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1359
                     /locus_tag="VD791_12620"
     CDS             217..1359
                     /locus_tag="VD791_12620"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HXV08870.1"
                     /translation="MLCIQRHVTGRLFPVGVLDPNPFDVPQHAIAEPALRQLTIPFIG
                     ADDRRAWIQLVTTSLLFIAGWAAMAVGVRSGWHYGLVLLLALPVAGLSVRLFILQHDC
                     GHGSFFSGRRLNDAVGRVLGVVTLMPYGYWRYTHAIHHATSGNLDRRGIGEIATRTVR
                     EYSQSGWWRRIGYRFYRSMPVLLGLGPLYQFVLKHRLPLDLPRSRRREWASVWINNVM
                     LAAAVAALSVAIGWKTLLLVHVPVLLVSGAAGVWLFYVQHQFENAYWARSDAWSVERS
                     AVAGSSHYDLPAVLRWFSANIGYHHLHHMATRIPNYRLRECHDSHPRLRQAPRLTLWT
                     SLRTARLRLWDEAAQRMVPFSAVAPGQAARRRRGLRGCEPRDRRVS"
     gene            1434..2156
                     /locus_tag="VD791_12625"
     CDS             1434..2156
                     /locus_tag="VD791_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXV08871.1"
                     /translation="MEPGAATPRQNQLLAALPAAEWERLRSKMELRPMPLGDVLYEPG
                     TTMRHVYFPTTSIVSLLYVMENGSSAEIAIVGHEGIVGISLFMGGETTSSRAVVQSAG
                     HAYRVLGQYLKEEFFLAGPFQRLLLRYTQALITQMGQTAVCNRHHSVEQQLCRWLLMS
                     LDRLPSNELTMTQDLIANMLGVRREGVTESAGRLQKLGLIQYNRGHIIVLDRPRLEAH
                     ACECYDVVKKEFDRLLPVREAT"
BASE COUNT          364 a          801 c          820 g          431 t
ORIGIN      
        1 cccagtatca ggtttgcggc accaccgagg agcgcgccgg cgaggatcca gatcactaca
       61 ctcacgtacg gctctcctgg ttgcggttga ctggcgcgag gttagtcgcc gccggccgcg
      121 ctgtcggtgc gctggcgcac ccagcggatc cggctgcctg atggccgtcc tatgtgtggt
      181 accgtacaga ccgcctgcgg ccggcagagc aggctcatgc tgtgtataca gcgccatgtg
      241 accggtcgac tatttccagt gggtgtgctt gatcccaatc cgttcgacgt tccccagcac
      301 gcgattgccg agcccgcatt gcgccagctg accatcccgt tcatcggagc ggacgatcgt
      361 cgtgcctgga tccagctggt gacgaccagc ctgctgttca tcgccgggtg ggccgcgatg
      421 gcggtcggcg ttcggtccgg ctggcactac gggctcgtcc tgttgctggc gctgccggtc
      481 gcagggcttt ccgtcaggct cttcatcctc cagcacgatt gcggccatgg ctcgttcttc
      541 agcggccgcc gcctgaacga cgcggtcggg cgcgtgctcg gcgtggtcac gctcatgccg
      601 tacgggtact ggcggtatac gcacgccatc caccatgcga cgtccggcaa cctggaccgc
      661 cgcgggatcg gcgaaatcgc cacgcgcacc gtgcgcgagt attcgcagtc gggctggtgg
      721 aggcgaatcg gctatcgctt ctaccgcagc atgccggtgc tgctcggtct cgggcccctg
      781 taccagttcg tgctcaagca tcgcctgccg ctcgacctgc cgcgctcgcg gcgcagggaa
      841 tgggccagcg tctggatcaa caacgtcatg ctggcggccg ccgtcgcggc tctctcagtg
      901 gcgatcggct ggaagaccct gctcctggtg cacgtgccgg tactgctggt gtcgggggcg
      961 gccggcgtgt ggctgttcta cgtgcagcac cagttcgaga acgcctactg ggcgagaagc
     1021 gatgcctgga gcgtggaacg ctcggccgtc gctggcagtt cccattacga tctgccggcg
     1081 gtcctgcgct ggttcagcgc caacatcggc taccaccacc tgcatcacat ggcgacgcgc
     1141 attccgaatt accgcctgcg cgagtgccac gactcgcacc cgcgactgcg gcaggcaccc
     1201 cggctgacgc tctggaccag cttgcgaacc gcgcggctgc ggctctggga cgaggcagcg
     1261 cagcgcatgg tgccgttctc tgcggttgcg ccggggcagg ccgcgcggcg gcggcgcggg
     1321 ctgcggggat gcgagccacg ggaccggcgc gtatcatgac ttcgagctcc aacccggggg
     1381 aatcgccgca cgacggccgc ccgattgccc aggccggcga gttcgggagt tcgatggaac
     1441 cgggtgccgc gacgccgagg cagaaccagc tgctcgccgc attgcctgcg gcggaatggg
     1501 agcggctcag gtcgaagatg gaattgcggc cgatgccgct cggagacgtg ctgtatgagc
     1561 ccggcaccac gatgcggcac gtgtactttc ccacgacctc gatcgtctcc ctcctgtacg
     1621 tgatggagaa cgggtcgtcc gcggagatcg ccatcgtcgg ccacgaaggc atcgtcggca
     1681 tttcgctgtt catgggcggc gagaccacct cgagccgcgc cgtcgtgcag agcgcgggcc
     1741 atgcctaccg tgtgctgggc cagtatctca aggaggagtt ttttctcgcc ggcccgttcc
     1801 agcggctgct gctgcggtac acgcaggcac tgatcacgca gatggggcag acggcggtct
     1861 gcaaccgtca ccactccgtc gagcagcagc tctgccgatg gctgctgatg agcctggatc
     1921 gcctgccttc caatgaactc acgatgacgc aggacctgat cgcgaacatg ctgggcgtgc
     1981 gcagggaagg cgtcaccgag tccgccggca ggctgcagaa gctgggactg atccagtaca
     2041 accgcggcca catcatcgtc ttggaccggc cccgcctgga agcgcacgcc tgcgagtgct
     2101 acgacgtggt caagaaggag ttcgatcgtt tgctgccggt gcgcgaggcg acctgagcga
     2161 tcggtcgtcc ggaccttgta acgcggggat ccacggctgc acctgactgc cttcggcgcg
     2221 catccgcagc gcggcggaag ccgatttcgc gccgtcaggt cgcgcccttg tagagactgt
     2281 cgagaccgcc gcgcagcgcc tgggcccagg tgcggatggc gcgacgcgca tccgccgtgt
     2341 tcgtgacccg gttggtgatc tgcatcatcc cggaattgcg gcccgaacgc gtgtcgacga
     2401 cccgtgcgag gacttc
//
LOCUS       DASPGE010000389         8454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_805322, whole genome shotgun sequence.
ACCESSION   DASPGE010000389 DASPGE010000000
VERSION     DASPGE010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8454
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805322"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /gene="ftsH"
                     /locus_tag="VD791_12630"
     CDS             <1..1503
                     /gene="ftsH"
                     /locus_tag="VD791_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HXV08872.1"
                     /translation="IGWLPMLLIVGLWFMLFRQLQSGGGKAMSFGKSRARLLNENEQR
                     VTFADVAGVDEAKEELKEVVAFLKAPKEYGRLGARVPKGVLLVGPPGTGKTLLARAVA
                     GEAGVPFFSITGSEFVEMFVGVGAARVRDLFEQARQKAPAIVFIDELDALGRARGAYP
                     MGGHDEREQTLNQLLAEIDGFDAGTGVVLLAATNRPEILDPALLRAGRFDRQILVDRP
                     DKAGRLQILQVHVKKVQLSRDVDLAKIAALTPGFAGADLANLVNEAALLATRRRGEAV
                     TLDDFTMAIERIVAGLEKKNRVLNEKERTVVAHHEMGHALVAMSLPGVDEVHKVSVIP
                     RGIGALGYTIQRPTEDRFLMTRAELEAKMAVLLGGRAAEHLMFGHTSTGAADDLVKVT
                     GIARSMVMRYGMSDKLGPVAYDQERTPFLEGPRVPVAQEFSEDTAREIDREVRDIVGR
                     VFEQAVSILARNRALLEKSARELLQKETLGEDDLRAIRAQVVAGATAAAA"
     gene            complement(1526..2164)
                     /locus_tag="VD791_12635"
     CDS             complement(1526..2164)
                     /locus_tag="VD791_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08873.1"
                     /translation="MGRIAMLDSVHAAATAILLAVAFLGAACASNGMQAQWSDPQFAG
                     RSLKGAKVLVVCEAAEVALQRICEDQVASRLGGSGVNPVRAADAGLAAGPAKPDRALD
                     AARSAGAAAIFTSTIAPDATVASPRPSIGIGIGGFGGSHSGVGAGVGVSVPVGTDRVS
                     TAYGASMSLIDTGTGKLMWSGRATAAASKDVAAQIGKLTDQGIRSAQKAGLL"
     gene            complement(2226..4145)
                     /locus_tag="VD791_12640"
     CDS             complement(2226..4145)
                     /locus_tag="VD791_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV08874.1"
                     /translation="MINKLEHMEFPAQIGRYETRRVVGRGGMAIVLEAWDPVLHRTLA
                     VKLVDKARLDLGVAPDALRRFKREAQAAARLTHPNVVQVYEYGEEGQFAWIAMEYVAG
                     KPLTAYLEEGLRTEVQRVRDVVGQLLEALAYSHTQGVVHRDIKPANLLVTPEGQIKVT
                     DFGIARVEASNLTQRGDMLGTPSYMSPEQYMGEASDGRTDIFSAGVILYELLTGQRPF
                     KGDSSGQIMQRILHDTPVNPSELNPQVPPPLDWVVQKALAKEAQDRFQSARDFAAALY
                     SAVPDVQAYDAGGQEVPRANQGLANAARKLRGLGRGGEAPGPGGAAPVFAPEATQQQT
                     QAKTRILFVDDEERILTALKSIFRGKYHVLTAANGQEALDFIRRFRIPVIVSDQRMPG
                     MLGVDLLRRSREISPDSVRILLTGYSDLASIVGSINDGEVYRFISKPWDNQELQQVIA
                     EATAIGNRMLELQRGRLEPPARLDGAVLVLDDRREVFNALASMAGDKVTLQHARNIQE
                     ALTCLEASDVAILVADIDMEAENYLVFFNLLKSKYPQILVLVMTSASDSELVIHLINE
                     AQIYRFLNKPVNLSLMQQYLHSAIGRHAEYRSAPELLERHRVRDSRSQETSFGKMVLD
                     RLKALKLPFAPARGS"
     gene            complement(4142..5968)
                     /locus_tag="VD791_12645"
     CDS             complement(4142..5968)
                     /locus_tag="VD791_12645"
                     /inference="COORDINATES: protein motif:HMM:NF039312.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV08875.1"
                     /translation="MNPATKRLLGGLIRVLGALLAVGILAFLYVKSESGELRRQTQVG
                     THLRQLREIDAGWSRDLAAARNDPFAAEAPPRPGGQRLPEVLDALQMETAALQDATLE
                     EGFASLRQSFHEKQGMMERFAQEAERLRTGVHAFLLQLGAVRQAVGEAAAADPRLRLG
                     ELDGRLVSLNGEILRWYVQTTPALRDAIRSTAGALQQQADGLPETLQAPVAALATSAG
                     PLLESEAALGRLVQEIDRLPAGPRVVSMTSAYERAFQAMADEKEQYRVYLVFYSAALL
                     VFLAYVLSQLGRSYVKLNQANEALRASNENLEQRVAERTRELSQALKHLKESELLLVQ
                     TEKMSSLGQMVAGIAHEINTPLAYVKSSLSTLQERLPAMQGVVQECLRLIQMVERGDA
                     SDEALTAQFNRVTTLAGEFHAEEGTAELQTINTDALHGIEQISEIILNLKNFSRLDRS
                     KVSRFDLNEGLESTLVIARNMVKHKTIKRELGKIPLIECSPSQVNQVFLNLITNAAQA
                     TRDDQSGQIALRSSVTADGYVRVDVSDNGHGIPENVLPKIFDPFFTTKDVGKGTGLGL
                     SIAYKIVQEHGGRIEVRSKVGQGTTFSVLLPVKATQKQALAA"
     gene            6158..>8454
                     /gene="mutS"
                     /locus_tag="VD791_12650"
     CDS             6158..>8454
                     /gene="mutS"
                     /locus_tag="VD791_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006408198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HXV08876.1"
                     /translation="MMQQYLRIKAQYRDMLLFYRMGDFYELFYGDAERAAALLDITLT
                     ARGVSGGEPVKMAGVPYHAVDQYLLKLVKLGESVAICEQIGDPALAKGPVERRVTRVI
                     TPGTLTDAALLEEKRDNVLLALATAGDTLGLAWLSLASGDFRVMEIAGERLAAELTRL
                     QPAEILLADAAEAALPPGLGAAIKRVPAWQFDRDGARRALARHFGTQDLSGFGAESLG
                     PALGAAGALLEYCRATQGAAPAHVLALRVEHESEYLRLDAVTRRNLEISETLRGDQAP
                     TLLSLLDTCATGMGSRWLRHALHHPLRDRAQAEARFEAVTALAGAGGDGPWRRAHALL
                     ARCADVERITARISLRSARPRDLSGLRETLALLPQLNAALAELAGVRIEQLATALQPR
                     QMLHEWLRSAIRDDPSAVVREGGVIAEGYDAELDELRAIQNDCGAFLLDLESRERART
                     GIANLRVEYNRVHGFYIEVTHANAAKVPDDYRRRQTLKNAERYITPELKAFEDKALSA
                     QERALAREKLLYDELLERMAAHIPDLRALAAALAELDVLAAFAERSLALDLVRPSFTE
                     EERIEIEAGRHPVVERQVDRFVANDLRLDPLRRLLLITGPNMGGKSTYMRQAAHIVLL
                     AHCGCFVPARAARIGSVDALFTRIGAADDLAGGRSTFMLEMTESAEILHQATRRSLVL
                     MDEVGRGTSTFDGMALAWAIVRHLVEKNQAYTLFATHYFELTALAQEYREVANVHLDA
                     VEHRDKIVFLHALEEGPASQSYGIQV"
BASE COUNT         1235 a         2952 c         2929 g         1338 t
ORIGIN      
        1 atcggctggc ttcccatgct gctgatcgtg ggtctgtggt tcatgctgtt ccgccagctc
       61 cagtcgggcg gcggcaaggc catgagcttc ggcaagtcgc gtgcgcggct gctcaacgag
      121 aacgagcagc gcgtcacctt cgccgacgtc gcgggcgtgg acgaggccaa ggaagagttg
      181 aaggaggtgg tggccttcct caaggcgccc aaggaatacg gccggctggg cgcgcgcgtg
      241 cccaagggcg tgctgctggt gggcccgccc ggcaccggca agacgctgct cgcgcgcgcg
      301 gtggccggcg aggccggcgt accgttcttc tcgatcaccg gatccgagtt cgtggagatg
      361 ttcgtcggcg tgggcgcggc acgcgtgcgc gacctgttcg agcaggcgcg gcagaaggcc
      421 cccgcgatcg tgttcatcga cgagctggac gcgctcgggc gcgcgcgcgg cgcctacccc
      481 atgggcggcc atgacgagcg cgagcagacc ctgaaccagc tgctcgccga gatcgacggc
      541 ttcgacgccg gcaccggggt ggtgctgctc gccgccacca accgacccga gatcctcgac
      601 ccggcgctgc tgcgcgcggg ccgtttcgac cggcagatcc tggtggaccg tccggacaag
      661 gccgggcgcc tgcagatcct gcaggtgcat gtcaagaagg tgcagctctc acgcgacgtg
      721 gatctcgcca agatcgccgc gctcaccccc ggttttgccg gcgcggacct cgccaacctg
      781 gtcaacgaag cggcgctgct ggccacgcgc cgacgcggcg aggccgtgac cctggacgac
      841 ttcaccatgg cgatcgagcg catcgtcgcc ggcctggaga agaagaaccg cgtgctgaac
      901 gagaaggagc gcacggtggt ggcgcatcac gagatggggc atgcgctggt cgcgatgtcg
      961 ctccccggtg tcgacgaggt gcacaaggtg tcggtgatcc cgcgcggcat cggtgcgctg
     1021 ggctacacca tccagcgccc caccgaggac cgcttcctga tgacgcgcgc ggagctcgag
     1081 gcgaagatgg ccgtgctgct gggcgggcgc gccgccgagc acctgatgtt cgggcacacc
     1141 tccaccggcg ccgccgacga cctggtgaag gtcaccggca tcgcgcgcag catggtgatg
     1201 cggtacggca tgtcggacaa gctcgggccg gtggcctacg atcaggagcg cacgcccttc
     1261 ctggaaggac cacgcgtgcc ggtggcgcag gagttcagcg aggacaccgc gcgcgagatc
     1321 gaccgcgagg tgcgcgacat cgtcggccgg gtgttcgagc aggcggtgtc gatcctcgcg
     1381 cgtaaccgcg ccctgctgga gaagagcgcg cgcgagctgc tgcagaagga gacgctcgga
     1441 gaagacgacc tgcgcgcgat ccgcgcgcag gtggtcgccg gcgccaccgc ggcggcggct
     1501 tgagtttgac aacgaccgcc gcgggttaca gcaagccggc tttctgcgcc gagcggatgc
     1561 cttggtcggt gagcttgccg atctgagcgg ccacgtcttt ggaggccgcg gcagtggctc
     1621 tgccgctcca catcagcttg ccggtgccgg tgtcgatcag cgacatgctg gccccatacg
     1681 ccgtgctcac ccggtcggtt cccaccggaa cgctcacacc gacgccagcg ccgacgccgc
     1741 tgtgggaacc gccgaagccg ccgatcccga ttccgatgct cggcctggga ctcgcaaccg
     1801 tcgcgtccgg ggctatcgta gaagtgaaga tcgcggccgc accggcgctg cgcgccgcgt
     1861 cgagcgcgcg gtcgggcttg gcgggtccgg ccgcgagacc ggcatcggct gcccgcaccg
     1921 gattcacccc tgatccgccg aggcgcgacg ccacctgatc ctcgcaaatg cgctgcaggg
     1981 cgacctcggc cgcttcgcac acgaccagca ctttcgcgcc tttgagcgag cgtccggcaa
     2041 actgcggatc gctccattgc gcctgcatgc cgttcgacgc gcatgcggcg ccgaggaacg
     2101 caaccgcgag cagaatcgct gttgcggccg cgtgcacact gtccaacatg gcgatgcgtc
     2161 ccatgcaggt tggaacacga taccggccga cggttccact gccccgtccg ataccgctgt
     2221 cctcgctacg agcctctggc cggcgcgaac ggcagcttca aggccttcag ccggtccagc
     2281 accatcttgc cgaaagaggt ctcctggctg cggctgtcgc gtacgcggtg gcgctcgagc
     2341 agctcgggcg cgctgcggta ctcggcgtgg cggccgatgg ccgagtgcag gtactgctgc
     2401 atcaggctca ggttcaccgg cttgttcagg aagcggtaga tctgcgcctc gttgatcagg
     2461 tggatcacca gctccgagtc cgacgcgctg gtcatcacca gcaccaggat ctgcgggtac
     2521 ttgctcttca gcaggttgaa gaacaccagg tagttctccg cctccatgtc gatgtcggcg
     2581 accagtatcg ccacatcgct ggcctcgagg caggtcagcg cctcctggat gttgcgcgca
     2641 tgctgcagcg tcaccttgtc cccggccatg ctcgccaggg cgttgaacac ctcgcgccgg
     2701 tcgtcgagca ccaggaccgc gccgtccagc ctggcgggcg gctcgagccg gccgcgctgc
     2761 agctcgagca tgcggttgcc gatggcggtg gcctcggcga tcacctgctg cagctcctgg
     2821 ttgtcccagg gcttgctgat gaagcggtac acctcgccat cgttgatcga gcccacgatc
     2881 gaggccaggt ccgagtagcc ggtcagcagg atgcgcacgc tgtccgggga gatctcgcgc
     2941 gagcgccgca gcaggtccac cccgagcatg cccggcatgc gctgatcgct gacgatcacc
     3001 gggatgcgga agcggcggat gaagtccagt gcctcctggc cgttggccgc ggtgagcacg
     3061 tggtacttgc cgcggaagat gctcttgagc gcggtgagga tgcgctcctc gtcgtccacg
     3121 aacaggatgc gcgtcttggc ctgggtctgc tgctgggtcg cctccggggc gaacaccggc
     3181 gcagcgccgc ccggtcccgg ggcttcgccg ccgcggccca ggccgcgcag cttgcgcgcg
     3241 gcgttggcga gcccctggtt ggcgcgcggg acctcctggc cgccggcgtc gtacgcctgc
     3301 acgtccggca ccgcgctgta cagtgcggcc gcgaaatcgc gtgcgctctg gaagcggtcc
     3361 tgcgcctcct tggcgagcgc cttctgcacc acccagtcca gcggcggcgg cacctgcggg
     3421 ttgagctcgg acggattcac cggcgtgtcg tgcaggatgc gctgcatgat ctgcccgctg
     3481 ctgtcgccct tgaacgggcg ctgcccggtc agcagctcgt acaggatgac gccggccgag
     3541 aagatgtcgg tgcgcccgtc ggaggcctcg cccatgtact gctcgggcga catgtagctg
     3601 ggcgtgccca gcatgtcgcc gcgctgcgtg agattggacg cctcgacgcg ggcgatgccg
     3661 aagtcggtga ccttgatctg gccttcgggc gtgaccagca ggttggccgg cttgatgtcc
     3721 cggtgcacca cgccctgggt gtgcgagtac gccagcgcct cgagcagctg cccgacgacg
     3781 tcgcgcacgc gctgcacctc ggtgcgcagc ccttcctcga gataggcggt gagcggcttg
     3841 cccgccacgt attccatggc gatccaggcg aactgccctt cctcgccgta ctcgtagacc
     3901 tgcaccacgt tgggatgcgt cagccgcgcc gccgcctgcg cctcgcgctt gaagcggcgc
     3961 agcgcgtcgg gcgcgacgcc gaggtccagg cgcgccttgt ccaccagctt gaccgccagc
     4021 gtgcggtgca gcacggggtc ccaggcctcc agcacgatcg ccatgccgcc gcgaccgacc
     4081 acgcgacgcg tctcgtagcg gccgatctgc gccggaaatt ccatgtgctc gagcttgttg
     4141 atcacgccgc gagcgcctgc ttctgggtgg ccttcaccgg cagcagcacc gagaaggtgg
     4201 tgccctgccc caccttggag cgcacctcga tgcgcccgcc gtgctcctgc acgatcttgt
     4261 aggcgatgga cagccccagc ccggtgcctt tgcccacgtc cttggtggtg aagaacgggt
     4321 cgaagatctt cggcaggacg ttttcgggaa tgccgtggcc gttgtcggag acgtccaccc
     4381 gcacgtagcc gtcggcggtg acgctggagc gcagcgcgat ctggccgctc tggtcgtcgc
     4441 gtgtggcctg cgccgcgttg gtgatgaggt tgaggaacac ctggttgacc tgcgacggcg
     4501 agcactcgat cagcgggatc ttgcccagct cgcgcttgat cgtcttgtgc ttgaccatgt
     4561 tgcgggcgat caccagcgtg ctctccaggc cctcgttcag atcgaagcgg ctcaccttgc
     4621 tgcggtccag gcggctgaag ttcttcaggt tgaggatgat ctcggagatc tgctcgatgc
     4681 cgtgcagcgc gtcggtattg atcgtctgca gctcggcggt gccctcctcg gcgtggaact
     4741 cgccggcgag cgtggtcacc cggttgaact gcgcggtcag cgcctcgtcg gatgcgtcgc
     4801 cgcgctcgac catctggatc aggcgcaggc attcctgcac caccccctgc atcgcaggca
     4861 gccgctcctg cagcgtggac aggctgctct tcacgtaggc gagcggggtg ttgatctcgt
     4921 gcgcgatgcc cgccaccatc tgccccagcg aggacatctt ctcggtttgc accagcagca
     4981 gctcggactc ctttagatgc ttgagcgcct gcgaaagctc ccgggtgcgc tcggccacgc
     5041 gctgctcgag gttctcgttg gaggcgcgca gcgcctcgtt cgcctggttc agcttgacgt
     5101 agctgcggcc gagctgcgac agcacgtagg ccaggaacac cagcaacgcc gccgaataga
     5161 acaccaggta gacgcggtac tgttccttct cgtctgccat cgcctggaat gcgcgctcgt
     5221 aggcgctggt catggacacc acgcgtggcc cggcgggcag gcgatcgatc tcctgcacca
     5281 gccggccgag cgccgcttcg ctctcgagca gcggccccgc cgacgtggca agcgccgcca
     5341 caggcgcctg tagcgtctcg ggcagcccgt ccgcctgctg ctgcaaggcg ccggcggtgg
     5401 accggatggc atcgcgcagc gccggggtgg tttggacgta ccagcgcagg atttcgccat
     5461 ttagcgacac cagccgcccg tccagctcgc ccagccgcag gcgcgggtcg gcggcggccg
     5521 cctcccccac cgcctgccgg acggcgccga gctgcaagag gaacgcgtgt acaccggtgc
     5581 gtagccgctc ggcctcctgc gcgaaccgct ccatcatgcc ctgcttctcg tggaagctct
     5641 ggcgcaggct ggcgaagccc tcttccaggg tcgcgtcctg cagcgcggcg gtttccatct
     5701 gcagcgcgtc cagcacctcc ggcaggcgct gcccgccggg acgcgggggc gcttcggcgg
     5761 cgaacgggtc gttgcgggcg gcggcgaggt cgcggctcca gccggcatcg atctcgcgca
     5821 gctgccgcag gtgggtgccg acctgcgtct gccggcgcag ttcgccgctc tcgctcttga
     5881 cgtacaggaa ggccaggatg ccgaccgcca ggagcgcgcc gagcacgcgg atcagcccgc
     5941 ctagcagccg tttcgtcgcc gggttcaaag gtggcagtcg tcgtggagga ggaggtacgc
     6001 cgatgccgca gggttgaggc agcgccgtat tatcggcaaa atgcacgagg cgtgccagat
     6061 agttgacaat acgcgcgctt ttttcagatc cgcgtcagac cgagcgaagg gtctggcgcc
     6121 ggaccgcacg caccccgtga gcgcccagca caccccgatg atgcagcagt acctgcgcat
     6181 caaggcgcag taccgcgaca tgctgctgtt ttaccgcatg ggcgatttct acgagctgtt
     6241 ctacggcgac gccgagcgcg cggccgcgct gctggacatc accctgaccg cccgcggcgt
     6301 ctcgggcggg gagccggtca agatggcggg cgtgccctac cacgcggtgg accagtacct
     6361 gctcaagctg gtcaagctgg gcgaatcggt ggccatctgc gagcagatcg gcgatcccgc
     6421 gctcgccaag ggaccggtgg agcgccgggt cacgcgcgtg atcacgcccg gcaccttgac
     6481 cgacgccgcc ctgctcgagg aaaagcgcga caacgtcctg ctcgccctgg ccacggccgg
     6541 cgacacgctg gggctcgcct ggctcagcct cgcgtccggc gatttccggg tgatggagat
     6601 cgcgggcgag cgcctcgcgg cggaactcac gcggctccaa cccgccgaga tcctgctggc
     6661 cgatgcggcc gaggccgcgt tgccccccgg cctcggcgct gcgatcaaac gcgttcccgc
     6721 ctggcagttc gaccgggacg gcgcacggcg cgccctcgcc cggcatttcg gcactcagga
     6781 cctcagcggt ttcggcgccg agtcgctcgg gccggcgctc ggcgccgcag gcgcgctgct
     6841 ggagtattgc cgtgccacgc agggcgccgc gccggcccac gtgctggcgc tgcgcgtcga
     6901 gcacgaatcc gagtacctgc gcctggatgc cgtcacgcgc cgcaacctcg agatcagcga
     6961 gacgctgcgc ggggaccagg cgcccacgct gctgtcgctg ctggacacct gcgccaccgg
     7021 gatgggcagc cgctggctgc gccacgctct gcaccatccg ctgcgcgacc gggcgcaggc
     7081 cgaagcgcgc ttcgaggcgg tgacggcgct ggccggcgcc ggcggcgacg gcccctggcg
     7141 ccgggcgcac gccctgctcg cccgctgcgc cgacgtggaa cgcatcaccg cgcgcatctc
     7201 gctgcgcagc gcccgcccgc gcgacctctc gggtttgcgc gagaccctgg cgctgctgcc
     7261 gcagctcaac gccgcgctcg ccgaactggc cggcgtgcgc atcgagcagc tcgccacggc
     7321 gctgcagccg cggcagatgc tgcacgaatg gctgcgcagc gcgatccgcg acgatccgag
     7381 cgccgtggtg cgcgaaggcg gcgtcatcgc cgagggctac gatgccgagc tcgatgagct
     7441 gcgcgccatc cagaacgatt gcggcgcctt cctgctcgat ctcgagtcgc gggagcgcgc
     7501 gcgcaccggc atcgccaatc tgcgcgtcga gtacaaccgc gtgcacggct tctacatcga
     7561 ggtcacccac gccaacgccg ccaaagtgcc ggacgactac cggcgccgcc agacgctgaa
     7621 gaacgcggag cgctacatca cgccggagct gaaggccttc gaggacaagg ccctgtccgc
     7681 gcaggaacgc gcgctggcgc gcgagaagct gctgtacgac gagctgctgg agcgcatggc
     7741 ggcgcacatc ccggacctgc gcgcgctggc ggcggctctc gccgagctcg acgtgctcgc
     7801 tgccttcgcc gagcgctcgc tggcgctcga tctggtgcgc ccgtccttca ccgaggagga
     7861 gcgcatcgag atcgaggccg gccgccaccc ggtggtggag cggcaggtcg accgtttcgt
     7921 cgcgaacgac ttgcgcctgg acccgctgcg acggctgctg ttgatcaccg ggccgaacat
     7981 gggcggcaag tcgacgtaca tgcgccaggc cgcgcacatc gtgctgctcg cccactgcgg
     8041 ctgcttcgtg ccggcgcgag cggcgcgcat cgggtcggtg gacgcgctgt tcacgcgcat
     8101 cggcgcggcc gacgatctgg ccggcgggcg ctccactttc atgctggaga tgaccgagag
     8161 cgccgagatc ctgcaccagg ccacgcgccg cagcctggtg ctgatggacg aagtcggtcg
     8221 cggcacctcc acgttcgacg gcatggcgct ggcctgggcc atcgtccggc acctggtgga
     8281 aaagaaccag gcctacacgc tgttcgccac gcactacttc gagctcaccg cgctcgcaca
     8341 ggagtaccgc gaggtggcca acgtgcacct ggacgccgtc gagcaccgcg acaagatcgt
     8401 gttcctgcac gcgctcgagg agggtccggc ctcgcagagc tacggcatcc aggt
//
LOCUS       DASPGE010000390        10054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1038213, whole genome shotgun sequence.
ACCESSION   DASPGE010000390 DASPGE010000000
VERSION     DASPGE010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10054
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038213"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VD791_12655"
     CDS             <1..1053
                     /locus_tag="VD791_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HXV08877.1"
                     /translation="NSLIPVDWDALPGWTQDRVAEAWGAFQRSCSLLGAQPAWRAACE
                     ASAAVDAADDTAVRAFFESAFTPHAVVNPDGTREGTITGYYEPVLRGSRTPDPVHRFP
                     AYAVPDDLLVVDLSELYPELKPMRLRGRIDGRRVVPYYSRGDLESGKADLSGREILWT
                     EDAVELFFLQIQGSGQVILDSGETVRLSYAEQNGHPYRSIGRLLVERGELTLDQASMQ
                     GIKAWAARNPERLSELLNQNASFVFFRELPATDGGPPGALGVGLTAGRSLAVDPRAIP
                     LGAPVFIATTWPNSEVPLQRLMLAQDTGGAIKGAVRADFYWGSGNEAGVQAGRMRQMG
                     QLYVLLPQGFPLNGVP"
     gene            complement(1037..2128)
                     /gene="ydiK"
                     /locus_tag="VD791_12660"
     CDS             complement(1037..2128)
                     /gene="ydiK"
                     /locus_tag="VD791_12660"
                     /inference="COORDINATES: protein motif:HMM:NF008216.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HXV08878.1"
                     /translation="MPDARNDLTRILLAVLSIGALIAGSLWVLWPFVGAVIWATMVVV
                     ATWPMMKGLQARLWNRRWLAVSVMTLALLLLLIVPLTLAVATIVENADRIVGWAKLLT
                     HFEIPPSPDWLTGLPMIGPNAGRLWEQLRAAGLGSLAAKIAPYAGGVAAWFAAQAGSA
                     GLITMQFLLTVVIAAIMYLHGESAASSVLRFGRRLAGERGETVILLAGRAIRGVALGV
                     VVTALVQSVLGGVGLAVCGVPFAVLLSGVMFILCIAQLGPALVLAPAVIWLYWTGDSF
                     WGTVLLVWTVVVVTLDNVLRPILIRRGADLPLLLIFAGVIGGLMALGLIGIFVGPVVL
                     AVTYTLLEAWLSDAPPPPAAGEPGPTARR"
     gene            complement(2200..3168)
                     /locus_tag="VD791_12665"
     CDS             complement(2200..3168)
                     /locus_tag="VD791_12665"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HXV08879.1"
                     /translation="MHLRWLRILALGAGLAAGAPSQADVLTMRSGEKLVGTVVEESAD
                     EIVFDSEAFGRLYIERARVLSLERGAEAAAAPTATPAPAPEPAEPTSGTLAFLARINP
                     LKGWKSSFHLGFVARRGDDSDNDLVLRFRSERTTAAGNDHRLQARYYYAEDVFSDDVQ
                     VETDNQLTADYRYRHRLTDPFFLQSDSRYYRDAITQMDHEVTQTVGIGYRAERERWSL
                     SLTPAAGVQWRELAGEDSTHAVVGLYQTFDIELTQTMKLRQDFDYLVAVDDSDDWSSR
                     FGLELTQKIGAAWSLALRYDFIYDSIVGKDASEDQQRLAFTLGVDF"
     gene            complement(3210..3863)
                     /locus_tag="VD791_12670"
     CDS             complement(3210..3863)
                     /locus_tag="VD791_12670"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXV08880.1"
                     /translation="MADADARLALAVLLSAALHASLIFGVAVRRPSMAPASEPILARI
                     EAVPDAAARPPEPPREEPPRRAARPAARTPAVAESPADASSRANLVRRAQQATPETLP
                     PVEMPLLVDPTWYPAAQLDVFPTALAPVRPAYPQAAADADVGGKVTLLLLIDEAGHVH
                     DSAVVDAQPANTFEEAALEAFRAARFRPGERDGRSVRSRVLVRVVFHPAEAGAAVSR"
     gene            complement(3980..4702)
                     /locus_tag="VD791_12675"
     CDS             complement(3980..4702)
                     /locus_tag="VD791_12675"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HXV08881.1"
                     /translation="MLTRFITLAVAACLLAASSMAGANEAAVRKAFAAKFPKAQVQSV
                     TKLPYLNLYEIVVEGEVLYADEKFEYVIDGSIISTKNMTNLTEQRKRKLTAIAFDELP
                     LDLAFKRVKGSGERKMAVFSDPDCPFCRRVENDLAKLDNVTIYMFLYPIESLHPKAPD
                     VSRRIWCSPDKIKAWDDYMQRGVQPGGDGECANPVDRIVEFGRKKGINGTPTLIFANG
                     DRVPGAISAAQIENYLSGKKEN"
     gene            complement(4802..5989)
                     /locus_tag="VD791_12680"
     CDS             complement(4802..5989)
                     /locus_tag="VD791_12680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HXV08882.1"
                     /translation="MDLDVAIVGGGLVGLSLACALDGGGLRVALVDRGAPPVAFPFET
                     AALPGSPGDWDVRVYAIGPGSQAFLTGCGAWPDTARIAPVDAMQVYGDAPSAGRLTFE
                     AHEARVAHLAQIVENRVLLDALWRRLRQSAASVLAPAQCRAVAFEQDAAVLTLEGQAP
                     LRARLLVAADGAQSWLRTQADMAASTTPYEHSAVVANFEAELVHRNSAFQWFRADGVL
                     ALLPLPGNRCSMVWSAQQGVADELMAASAHALAERVCEASRGALGRLRQITPPAAFPL
                     QLVRVPRLVRARLALVGDAAHNLHPLAGQGVNLGFQDARELAQVLLQRGACHDAGEIR
                     LLRRYERARREDILAMTWVTHGLQRLFGAAGRPASWVRNRGLSLVDRADPIKQLLVRH
                     ALG"
     gene            6256..6330
                     /locus_tag="VD791_12685"
     tRNA            6256..6330
                     /locus_tag="VD791_12685"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6288..6290,aa:Met,seq:cat)
     gene            6349..6798
                     /locus_tag="VD791_12690"
     CDS             6349..6798
                     /locus_tag="VD791_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08883.1"
                     /translation="MLQSFEVTLRSGARRITRFARRAAAVLLLGCVLALATSPDQGFA
                     ARNLPRDARFGKLTAFTYPYASIGGKTLRMSPGAKIYNEQNLIIMPAAMRQQAKVLYR
                     LDNAGSLSAIWLLTEHEAAPFERRFPPVKPAPDTGAASATAPAGSAQ"
     gene            6829..8157
                     /gene="miaB"
                     /locus_tag="VD791_12695"
     CDS             6829..8157
                     /gene="miaB"
                     /locus_tag="VD791_12695"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HXV08884.1"
                     /translation="MAGKFFIRTFGCQMNEYDSDKMADVLRVEQAMEATDDPAQADLI
                     LFNTCSVREKAQEKVFSDLGRVRALKRRNPALLIGVGGCVASQEGTAITARAPFVDLV
                     FGPQTLHRLPRMIEARRRSGRPQVDVSFPEIEKFDHLPPARVHGASAFVSIMEGCSKY
                     CSFCVVPYTRGEEVSRLFDDVLTEVAGLTDQGVKEITLLGQNVNAYRGRISDRETADL
                     ALLLEYLAEMPGLARIRYTTSHPREFTQRLIDAHAAIAQLAGHVHLPVQAGSDRVLAA
                     MKRGYTVLEYKSIVRRLRATRPQISITSDFIVGFPGETEEDFRATLRLVEEVGFDTSF
                     SFVYSRRPGTPAAGLPDDTPQALKLERLQRLQERLNALAQGIGRAMLGTRQSVLVEGR
                     SRKDADELCGRTENNRVVNFAGHRRLIGELTDVTVTAALAHSLRGEVVLR"
     gene            8167..9138
                     /locus_tag="VD791_12700"
     CDS             8167..9138
                     /locus_tag="VD791_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HXV08885.1"
                     /translation="MEISFTPVDNQRLANLCGVLDENLKQIESAFDVSIARRGESFSI
                     AGEPPRTELAAEALRRFYEQAGSELSVEDVQLGLIEVANPTGAEGAEQPVLITRRRDL
                     HARTPHQLQYLRHIREHDITFGIGPAGTGKTYLAVAAAVDAFERDAVKRIVLVRPAVE
                     AGERLGFLPGDLAQKVDPYLRPLYDALYDLMGFEKVGKLFERGSIEIAPLAYMRGRTL
                     NHSFIILDEAQNTTPEQMKMFLTRTGFGTKAVITGDITQIDLARGQKSGLVDAAEVLK
                     DVRGIAFTRFTSEDVVRHPLVARIVNAYEAASQRGARPPHDGQSKTS"
     gene            9146..9583
                     /gene="ybeY"
                     /locus_tag="VD791_12705"
     CDS             9146..9583
                     /gene="ybeY"
                     /locus_tag="VD791_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HXV08886.1"
                     /translation="MSTQFAVAARGRPSRRRLRAWAQAALERDVELTVRVVGRAEGRR
                     LNRDFRGRDRATNVLTFVYSEPGQPLAGDIVLCAPVVEAEARAQGKARDAHYAHLVVH
                     GMLHLQGYDHERSAAAAAMEARESEIVTRLGYADPYADDGPSA"
     gene            9546..>10054
                     /locus_tag="VD791_12710"
     CDS             9546..>10054
                     /locus_tag="VD791_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXV08887.1"
                     /translation="MLTRTPTMDPQPKPSLLERIGAILMREPEDRDQLVELLRSAYER
                     NLIDADALGMIEGALQVSELQARDIMVPRAQMDLVDLHEPPEQILPKVIEAGHSRFPV
                     YGDNKDDIVGVLLAKDLLRYFAGHEVSLREMLRPAVFVPESKRLNVLLRDFRRNRNHM
                     AIVVDEYGGV"
BASE COUNT         1596 a         3547 c         3428 g         1483 t
ORIGIN      
        1 aacagcctga tcccggtgga ctgggacgcg ctcccgggct ggacgcagga tcgcgtcgca
       61 gaagcctggg gcgcgttcca gcgcagctgc tcgctgctcg gcgcccagcc ggcctggcgc
      121 gccgcctgcg aagcttccgc cgcggtggac gcggccgacg acactgcggt gcgagccttc
      181 ttcgagagcg ccttcacgcc gcacgccgtg gtcaatccgg acggcacgcg cgaaggcacc
      241 atcaccggct actacgagcc ggtgctgcgc ggcagccgaa cgccggatcc agtccatcgc
      301 tttcctgcct acgccgtccc cgacgatctg ctggtggtgg acctgtccga gctgtacccc
      361 gagctcaagc cgatgcgcct gcgcgggcgc atcgatgggc gtcgcgtcgt gccctactat
      421 tcgcgcggcg acctcgagtc gggcaaggcc gacctgtccg gccgcgagat cctgtggacc
      481 gaggacgccg tggagctgtt cttcctgcag atccagggat cgggccaggt gatcctggac
      541 agcggcgaga cggtgcgtct gtcctacgcc gagcagaacg ggcatcccta ccgctcgatc
      601 gggcgcctgc tggtggaacg cggcgaactg acgctcgacc aggcctcgat gcagggcatc
      661 aaggcctggg ccgcgcgcaa cccggagcgc ctgtccgagc tgctcaacca gaacgcctcc
      721 ttcgtgttct tccgggagtt gcccgctacc gacggcgggc cgcccggtgc gctgggcgtg
      781 gggctgaccg ccggccgcag cctggccgtg gacccgcgcg ccatccctct gggcgcgccc
      841 gtgttcatcg ccacgacctg gccgaactcg gaggtgccgc tgcagcgcct gatgctggcc
      901 caggacaccg gcggcgcgat caagggcgcg gtgcgcgccg atttctattg ggggtcggga
      961 aacgaggcgg gcgttcaggc cggccgcatg cggcagatgg gccagctgta cgtgctgctg
     1021 ccgcaaggct tcccgctcaa cggcgtgccg tagggccagg ctcgcctgcc gcgggcggcg
     1081 gcggggcatc gctcagccat gcctcgagca gcgtataggt gacggcgagc accaccgggc
     1141 ccacgaagat gccgatcagg cccagggcca tcaggccgcc gatcacgccg gcgaagatca
     1201 gcagcagcgg cagatcggca ccccgccgga tcaggatcgg gcgcagcacg ttgtccagcg
     1261 tgaccacgac gacggtccac accagcagca ccgtccccca gaaggaatcg ccggtccagt
     1321 acagccagat cacggcgggc gccagcacca gcgccggccc gagctgagcg atgcacagga
     1381 tgaacatgac gccgctcagc agcaccgcga agggcacgcc gcacaccgcc aggccgacgc
     1441 cgccgagcac cgactgcacc agggcggtga cgaccacccc cagcgccacg ccgcggatgg
     1501 cgcggcccgc gagcaggatc acggtctcgc cgcgctcgcc cgcgagccgc cgaccgaagc
     1561 gcagaacgct ggacgcggcg ctttccccgt gcaggtacat gatcgcggcg atcaccacgg
     1621 tgagcaggaa ctgcatggtg atcagcccgg cgctgccggc ttgcgcggcg aaccaggcgg
     1681 ccacgccgcc ggcataggga gcgattttcg ccgcgaggct accgaggccc gcagccctca
     1741 actgctccca caacctgccc gcgttcggcc cgatcatcgg cagcccggtc agccaatcgg
     1801 gcgacggcgg tatctcgaag tgggtgagca acttcgccca gccaacgatc cgatccgcgt
     1861 tctcgacgat ggtggccacc gcgagcgtca gcggcacgat cagcagcagt agcagcgcca
     1921 gcgtcatcac gctcaccgcc agccagcgcc ggttccacaa gcgcgcctgc aggcctttca
     1981 tcatcggcca ggtggccacc accaccatgg tggcccagat gacggcgccg acgaatggcc
     2041 acagcaccca caacgagccc gcgatcagcg ccccgatgga cagcaccgcc aacaggatgc
     2101 gcgtgaggtc gttgcgggcg tcgggcacgt cgatgtgcgg ctcctcgatg ctgcgcggat
     2161 tctacgcgac cgccggcatc cggcccagcc cggcgccgct cagaaatcca ccccgagggt
     2221 gaaggccagc cgctgctggt cctccgaggc atccttgccc acgatgctgt cgtagatgaa
     2281 gtcgtagcgc agcgcgagcg accacgccgc cccgatcttc tgggtgagct ccaggccgaa
     2341 acggctcgac cagtcgtcgg aatcgtccac cgccaccagg tagtcgaagt cctgccgcag
     2401 cttcatggtt tgcgtcagct cgatatcgaa ggtctgatac aggccgacta ccgcgtgggt
     2461 ggagtcctct ccggcgagct cgcgccactg caccccggct gccggggtaa gcgacaggct
     2521 ccagcgctcg cgctcggctc gatagccgat gccgacggtt tgcgtcacct cgtggtccat
     2581 ctgcgtgatg gcgtcgcggt aatagcggct gtcgctctgc aggaagaacg ggtcggtaag
     2641 ccggtggcgg tagcgatagt cggcggtgag ctggttgtcc gtctcgacct ggacgtcatc
     2701 ggagaacacg tcttcggcgt agtagtagcg cgcctggagg cggtgatcgt tgccggccgc
     2761 cgtggtgcgc tcggagcgga agcgcaggac gaggtcgttg tcgctgtcgt cgccgcggcg
     2821 cgcaacgaaa ccgaggtgga agctgctctt ccagcctttc agcggattga tgcgcgccag
     2881 gaaggcgagc gtaccgctgg tcggctcggc gggttcgggc gccggtgcgg gcgttgctgt
     2941 cggcgcagcc gccgcttcgg cgccgcgctc caggctcaga acgcgtgcgc gctcgatata
     3001 caaccggccg aaggcctcgg agtcgaacac gatctcgtcg gcgctctcct ccaccacggt
     3061 tccgaccagc ttctcgccgc tgcgcatggt gagcacgtcg gcctggctcg gcgcgccggc
     3121 ggccagtccg gcgccgagcg ccaggatgcg aagccatcgc agatgcacgg cagttcgatc
     3181 gatcgcggtc ggctggaaag ccggcaatgc taccgtgata cggccgcgcc cgcctccgcc
     3241 gggtggaaca ccactcgcac caggacgcgg ctgcgcacgc tgcgtccgtc gcgctcgccg
     3301 ggccggaagc gcgctgcgcg aaacgcctcc aatgccgcct cttcgaaggt attcgccggc
     3361 tgcgcatcca ccaccgcgct gtcgtgcacg tgcccggcct cgtcgatcag cagcagcagc
     3421 gtcaccttcc ccccgacatc cgcgtcggcc gccgcctgcg gataggcggg ccgcaccggc
     3481 gcgagcgcgg taggaaacac gtcgagctgt gccgcggggt accaggtcgg gtcgacgagc
     3541 agcggcatct cgacgggagg cagcgtctcg ggcgtggcct gttgtgcgcg ccgaacgagg
     3601 ttcgctcggc tcgatgcatc ggccggggat tcggcgacgg cgggcgtgcg cgcggcgggc
     3661 cgtgcagcgc gtcgtggagg ctcctcacgc ggtggctcgg gcggacgagc cgccgcgtcg
     3721 ggcaccgctt cgatgcgggc gagaatcggc tcggaggcgg gagccatgct cgggcggcgc
     3781 acggccacgc cgaagatcag ggatgcatgc agcgcagcgg agagcaggac ggcgagcgcc
     3841 agccgcgcgt cggcatcggc cacggcatct gccttgtgcg cccttaaggc gcgacttaaa
     3901 aaacgttcgg cccctctcgc gaggcgggat gacgccacga cccacacccg tggtgtgcgg
     3961 cagccgtcat tcatccgcgt cagttctctt tcttgccgct caagtagttc tcgatctgcg
     4021 cggcggagat cgctcccggc acgcggtccc cgttggcgaa gatcagcgtc ggcgtgccgt
     4081 tgatgccctt cttgcgcccg aactccacga tccggtccac cgggttggcg cactcgccgt
     4141 cgccaccggg ctgcacgccg cgctgcatgt agtcgtccca cgccttgatc ttgtccggcg
     4201 agcaccagat gcgccgcgac acgtcgggag ctttcgggtg cagcgactcg atcgggtaga
     4261 ggaacatgta gatggtgacg ttgtcgagct tggccagatc gttctccacg cggcggcaga
     4321 acgggcaatc ggggtcggag aacaccgcca tcttgcgctc gccgctgccc ttgacccgct
     4381 tgaaggcgag atcgagcggc aactcgtcga aggcgatggc cgtgagcttg cgcttgcgct
     4441 gctcggtcag gttggtcatg ttcttggtcg agatgatgct gccgtcgatg acgtactcga
     4501 acttctcgtc ggcgtacagc acctcgccct cgaccacgat ctcgtacagg ttcaggtacg
     4561 gcagcttggt gacgctctgc acctgggcct tggggaactt ggccgcgaac gccttgcgca
     4621 ccgcggcctc gttggcgccg gccatgctgc ttgcggccag caagcaggcc gcgacggcca
     4681 aggtgatgaa tctcgtcagc atgtgggatc cttcaagtgg ggtggcgata gcgccgctac
     4741 ccgagcgccc tttacttacc cgagagcccg agcgcccttt acccgagagc ccgagcgccc
     4801 tttacccgag agcatggcgc accagcagct gcttgatcgg gtcggcgcgg tccaccagag
     4861 acagcccccg gtttcgaacc caggatgcgg gccgaccggc ggcgccgaac agccgctgca
     4921 agccgtgcgt cacccaggtc atggcgagga tgtcttcgcg gcgcgcgcgc tcgtaacggc
     4981 gcagcagccg gatctctccg gcgtcgtggc acgccccacg ctgtagcaga acttgcgcca
     5041 gctcgcgcgc atcctgaaac ccgaggttga ccccctgccc ggcgagcggg tgcagattat
     5101 gcgcggcgtc cccaaccagc gcaaggcgcg cccgcaccag cctcggcacg cgcacgagct
     5161 gcagcgggaa cgccgccggc ggcgtgatct ggcgcagccg gcccagcgcg ccccggctcg
     5221 cctcgcacac gcgctcggcc agagcgtggg cactcgcggc catcagctcg tccgccacgc
     5281 cctgctgcgc agaccacacc atcgagcaac ggttcccggg cagcggcaac agggccagca
     5341 cgccgtcggc gcggaaccat tggaaggcgc tgttgcggtg cacgagctcg gcttcgaagt
     5401 tggccacgac cgcgctgtgc tcgtaggggg tcgtggaggc cgccatgtcg gcttgggtgc
     5461 gcagccagga ctgggcgccg tcggccgcga ccaggagcct ggcgcgcagc ggcgcctggc
     5521 cctcgagcgt cagcaccgcc gcgtcctgct cgaacgcgac ggcccggcac tgcgcaggcg
     5581 ccagcaccga cgcggccgac tgtcgcaggc gccgccacag cgcatcgagg agcacgcggt
     5641 tctccacgat ctgcgcgagg tgcgctaccc gcgcttcgtg ggcctcgaac gtgagccggc
     5701 cggcgctggg ggcgtctccg tacacctgca tcgcgtccac cggcgcaatc cgcgccgtgt
     5761 cgggccaggc gccgcagccg gtcagaaagg cctggctccc ggggccgatg gcgtacacgc
     5821 gcacgtccca gtcgcccggc gagccgggca aggcagccgt ctcgaatgga aaagccacgg
     5881 gcggcgcgcc acgatcgacc agcgccacgc gcaacccgcc gccgtccagc gcacacgcca
     5941 ggctcaggcc gacgagcccg ccgccgacga tcgcgacatc gagatccaag gagtgcatgg
     6001 atgtatacgg ttcgacaatg cgcctgaagc aaccgttaca ggatcttacg cgcggctccc
     6061 attgctccag ccgacgccgc tcggattcgg taccatgtcg gaccgagcgc gccttgacag
     6121 tggcgcgcgg gctcacgtac cctttcgcgc cttgcttttc gggcactatg ggtacttcgg
     6181 cccatgccgg gtgtttcgac ccctgccgac cgttgaaaaa cccgaccgtt gaagaacccg
     6241 accgttgaaa aacgtggcga attagctcag cggtagagca gaggaatcat aatcctttgg
     6301 tcgggggttc aaatccctca ttcgccacca gcccccattt ccgcacgcgt gttgcagtct
     6361 ttcgaggtca ctctgcgcag cggcgcgcgc cgcatcacgc ggttcgcgcg ccgggcggcg
     6421 gcggtgctgc tgctcggctg tgtgctcgct ctggccactt cccccgacca ggggttcgcc
     6481 gcccgcaatc tgccccggga cgcgcgcttc ggcaagctca ccgccttcac ctacccctac
     6541 gccagcatcg gcggcaagac cctgcgcatg agcccggggg cgaagatcta caacgagcag
     6601 aacctgatca tcatgcccgc cgccatgcgc cagcaggcca aggtgctgta ccggctggac
     6661 aacgcgggca gcctgtccgc gatctggctg ctcaccgagc acgaggccgc ccccttcgag
     6721 cggcgcttcc cgccggtcaa accggctccc gacaccggcg ccgcttcggc caccgccccg
     6781 gccggcagcg cccagtagcg tccgcacgct ctcgcgcgtt ctcgggtcat ggccggcaag
     6841 ttcttcatcc gcaccttcgg ctgccagatg aacgagtacg actcggacaa gatggcggac
     6901 gtgctgcgcg tggagcaggc gatggaggcc accgacgatc ccgcgcaagc cgacctgatc
     6961 ctgttcaaca cctgctcggt gcgcgagaag gcgcaggaga aggtgttctc cgatctgggg
     7021 cgcgtgcgcg cgctgaagcg gcgcaatccc gccctgctga tcggggtggg cggctgcgtg
     7081 gccagccagg agggcacggc catcactgcg cgcgcgccgt tcgtagacct cgtgttcggc
     7141 ccgcagacgc tgcatcggct gccgcgcatg atcgaggcgc gacgccgctc cggccggccc
     7201 caggtggacg tgtcgttccc cgagatcgag aagttcgacc acctgccgcc ggcccgagtg
     7261 cacggcgcca gcgctttcgt ctcgatcatg gaaggctgca gcaagtactg cagcttctgc
     7321 gtggtgcctt atacgcgcgg cgaagaggtg tcgcggctct tcgacgacgt gctcacggag
     7381 gtcgccgggc tcaccgacca gggcgtgaag gagatcacgc tgctcggcca gaacgtgaac
     7441 gcctaccgcg gccggatctc cgatcgggaa acggccgacc tggcgctgct gctcgagtac
     7501 ctggcggaga tgccggggct cgcgcgcatc cgctacacca cttcgcaccc cagggagttc
     7561 acgcaacgcc tgatcgacgc tcacgccgcg atcgcgcagc tcgccggcca cgtgcacctg
     7621 ccggtgcagg ccggctcgga ccgcgtgctc gccgccatga agcgcggcta caccgtgctc
     7681 gagtacaagt ccatcgtgcg ccggctgcgc gcaacgcgcc cgcagatctc gatcacctcc
     7741 gacttcatcg tcggcttccc cggcgaaacc gaggaggatt tccgggcgac gctgcgcctg
     7801 gtggaggaag tcggcttcga caccagcttc agcttcgtgt acagccggcg cccgggcacg
     7861 ccggccgccg gcctgcccga cgatacgccg caggcgctca agctcgagcg gctgcagcgc
     7921 ctgcaggagc ggctcaacgc tctggcgcag ggtatcggcc gcgcgatgct gggcacccgc
     7981 cagagcgtgc tggtggaggg ccgctcccgc aaggacgccg acgagctttg cggtcgcacc
     8041 gagaacaatc gcgtggtcaa cttcgccggg catcggcgcc tgatcgggga gctcacggac
     8101 gtgaccgtca ccgccgccct ggcccactcc ctgcgcggtg aagtcgtgct gcgctgattg
     8161 gaatccgtgg agatctcctt caccccggtg gacaaccaga ggctcgccaa cctgtgcggc
     8221 gtgctcgacg agaacctcaa gcagatcgag tcggcgttcg acgtgagcat cgcgcggcgc
     8281 ggggagagct tcagcatcgc cggtgagccg ccgcgaaccg agctcgccgc cgaagcgctg
     8341 cgccgtttct acgaacaggc cggcagcgag ctgagcgtgg aggacgtgca gctcggcctg
     8401 atcgaagtgg ccaatcccac gggggcggag ggcgccgaac agccggtgct gatcacgcgc
     8461 cggcgcgacc tgcacgcgcg cacgccgcac cagttgcagt acctgcgcca tatccgcgag
     8521 cacgacatca ccttcggcat cggcccggcg ggcaccggca agacctacct ggcggtggcc
     8581 gccgcggtgg acgcgttcga gcgcgacgcc gtcaagcgca tcgtcctggt gcgcccggcg
     8641 gtggaggccg gcgagcgcct gggcttcctg ccgggcgacc tggcgcagaa ggtggacccc
     8701 tacctgcggc cgctgtacga cgcgctctac gatctgatgg gcttcgagaa ggtgggcaag
     8761 ctgttcgagc gcggcagcat cgagatcgcc ccgctcgcct acatgcgcgg gcgcacgctg
     8821 aaccattcct tcatcatcct ggacgaggcg cagaacacca cgcccgagca gatgaagatg
     8881 ttcctcaccc gcaccggctt cggcacgaag gcagtgatca ccggcgacat cacccagatc
     8941 gacttggcgc gcggccagaa gagcggcctg gtggacgcgg ccgaggtgct gaaggacgtg
     9001 cgcggcatcg cctttacccg cttcaccagc gaggacgtgg tacgccatcc cctggtggcg
     9061 cgcatcgtca acgcctacga ggcggcgtcc cagcgcggcg ctcgcccgcc gcacgatggg
     9121 cagtcgaaaa cctcctagcc tctcgatgag cacgcagttc gccgtcgcgg cccgcggccg
     9181 acccagccgc aggcggctgc gcgcgtgggc gcaggcggcg cttgagcgcg atgtcgagct
     9241 gaccgtgcgc gtggtgggcc gggcggaggg ccggcgcctc aaccgcgatt tccgcggccg
     9301 ggatcgcgcc accaacgtgc tcaccttcgt ctactccgaa ccgggacagc cgctcgccgg
     9361 cgacatcgtg ctgtgcgcgc cggtggtgga ggccgaggcg cgcgcgcaag gcaaggcgcg
     9421 cgacgcgcat tacgcccatc tggtagtgca cggcatgctg catctgcagg gctacgacca
     9481 cgagcgcagc gcggccgcgg cggcgatgga ggcgcgggag tctgaaatcg taacgcggct
     9541 ggggtatgct gacccgtacg ccgacgatgg accctcagcc taaacccagc ctcctggagc
     9601 gcatcggggc gatcctgatg cgcgagccgg aggaccgcga tcaactggtg gagctgctgc
     9661 gctcggcgta cgagcgcaac ctgatcgacg ccgacgcgct gggcatgatc gagggcgcgc
     9721 tgcaggtctc cgagctgcag gcgcgcgaca tcatggtgcc gcgcgcgcag atggatctgg
     9781 tggatctgca cgagccgccc gagcagatcc tgcccaaggt gatcgaggcg gggcactcgc
     9841 gcttcccggt gtacggcgac aacaaggacg acatcgtcgg cgtgctgctc gccaaggacc
     9901 tgctgcgcta tttcgccggc cacgaggtca gcctgcgcga gatgctgcgc ccggcggtgt
     9961 tcgtgcccga gtccaagcgc ctgaacgtgc tgctgcgcga tttccgccgc aaccgcaacc
    10021 acatggcgat cgtggtggac gagtacggcg gcgt
//
LOCUS       DASPGE010000391        11238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_571984, whole genome shotgun sequence.
ACCESSION   DASPGE010000391 DASPGE010000000
VERSION     DASPGE010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11238
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571984"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /gene="panC"
                     /locus_tag="VD791_12715"
     CDS             <1..808
                     /gene="panC"
                     /locus_tag="VD791_12715"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HXV08888.1"
                     /translation="RKRLRREDNVACVPTMGNIHDGHLSLVHIARRHAPLTVTTIFVN
                     RLQFGAGEDFDKYPRTFEEDCAKLEAAGNTIVFHPDEGEMYPEPQAFHVDPPAIANKL
                     EGRFRLGHFRGVATVVLKLINIVQPQTAVFGKKDYQQLAIVRHMVEQLALPVKIVPAE
                     TVRAPDGLALSSRNRYLSAVERAEAPRLQRVLQRVREQVAGGERNFEAIEFAADAELA
                     RHGWHVDYVVVRSRRTLMQPESGETGLVTLGAARLGNTRLIDNLEIDAPG"
     gene            complement(841..2481)
                     /locus_tag="VD791_12720"
     CDS             complement(841..2481)
                     /locus_tag="VD791_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(methylthio)propionyl-CoA ligase"
                     /protein_id="HXV08889.1"
                     /translation="MNGLMMNTPLQISSLIRHADRYHGDTEIVSRLVEGGIHRYTYRD
                     AHRRARQLANALGALGVRMHDRVGTLAWNGFRHFELYYAVSGMGAVIHTVNPRLFPEQ
                     IAYIVNHADDQYVCFDLTFAPLVERLAPHCAGVRGWVAMTDRAHMPKLPLPGLLCYED
                     LINAHSDGYDWPQFDEWTAAGLCYTSGTTGNPKGVLYAHRSTVLHAYAGCLPDALNLS
                     ARDVVLPVVPMFHVNAWGLPYACALAGSKLVFPGAQLDGASLYELFEREKVTMSAGVP
                     TVWLGLLQHVQQGGLAFSTFNRTVIGGSACPPAMIRTFEQDYGVRVLHAWGMTEMSPL
                     GTVCTLKAKHLALPKAEQLKVQDKQGRPLFGVDMQIVDDQGKALPWDGKAFGDLLVRG
                     PWVTTQYFKGEGGAILRQDECGASWFPTGDVATIDPDGYMQITDRSKDVIKSGGEWIS
                     SIDLENTAMAHPAVKEAAVVGVAHPKWDERPLLIVVKKPDASLTREELLAFFQGKVAK
                     WWVPDDVVFVAELPHTATGKLLKTRLREDYREYRLPGV"
     gene            2695..3642
                     /locus_tag="VD791_12725"
     CDS             2695..3642
                     /locus_tag="VD791_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV08890.1"
                     /translation="MEFFLVQLLNSLSYGLLLFMLSSGLTLIFSMMGVLNFAHASFYM
                     LGAYLAFQLSSHLGFWPALALAPLLVGALGALTERWGLRTVHKYGHVAELLFTFGLFY
                     LIEEVVHLMWGRAPVDYRIPASLDFSAFTLFSTSYPAYRVFMMAVSVGMLLALYLVLT
                     RTRIGMIIQASLSHPDMVEALGHNVPRVFMLVFGGGCALAGLAGVIGGNAFVTEPGMA
                     AAVGSIVFVVVVFGGMGSLTGAFVASLFFGAVQTFSVALDYSLLDLAGVLGVEVGAES
                     GWRRLLSITIAQTAPVLPYLLMVLMLIVRPTGLMGTRET"
     gene            3680..4945
                     /locus_tag="VD791_12730"
     CDS             3680..4945
                     /locus_tag="VD791_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV08891.1"
                     /translation="MTGKRDQILRRALLWGATAGVMIALPLLFRQGFALSMLSQMGIA
                     IVFALSYNMLLGQTGMLSFGHAVYYGLGAFVCAHTLNAVADGRFALPVTLVPLVGGVS
                     GLAFGIVFGYVTTKRSGTPFAMISLGIAEMVAACALMFPGFFGGEGGIATNRVMGEPW
                     HGLTYGPQVQVYYLIAGYCLACMIAMFALTQTPLGRMANAVRDNPERVRFVGYDPTVV
                     RYLVFCLASFFAGIAGGLGTVNYEIVTAENVGLLASGNVLVMTFVGGVGYFFGPVIGA
                     VLVSFMQSALSSYTQAWLLYFGLFFVVMVLFAPGGIASLIVMHGPLLRHRLWARLVPG
                     YLAAGAAFAVLLAGLIGLVEMIYHLERGAESGAAFRLFGQAVEVDLPGPWLAAGAGVL
                     LGLALLRPAIALARRNWVSVHAELQRAGA"
     gene            4942..5688
                     /locus_tag="VD791_12735"
     CDS             4942..5688
                     /locus_tag="VD791_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV08892.1"
                     /translation="MNNAIELRAVHKSFGRTAIINGVDLAVAPGERHAIIGPNGAGKS
                     TLFNLISGRFPVTSGRILLNGEDITGREPQEINHRGLARSFQITNIFPRLSVYENVRC
                     AVLRSMGYGYSFWHRVSALERVRRRAGEIVERIGLARRRDRAAGVLSYAEQRALEIGI
                     TIAGGADVILLDEPTAGMSRSETADAVELIRTVTGGKTLLMVEHDMGVVFGLADRISV
                     LVYGKVLATGTPQEIRANPAVQEAYLGTQH"
     gene            5726..6439
                     /locus_tag="VD791_12740"
     CDS             5726..6439
                     /locus_tag="VD791_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV08893.1"
                     /translation="MLEVRDLHAYYGKSHILHGVDMTVGQGEIVSLLGRNGAGRSTTV
                     RAVMGQVAKSGSVRLRGEEISGLRTYLIARRGLGYVPESREIFPTLTVRQNLMLGAKT
                     RARSSAGRAGGRWSMDDLFGLFPVLRERADAPAGVISGGEQQMLTLCRTLMGDPDLVM
                     IDEPTEGLAPKVVELVARLFDEMKGRGLSILLVEQKLTIALRLSQRLYVMGHGRIVFQ
                     GTPDDLRANEQVRKEWLEV"
     gene            6484..8583
                     /locus_tag="VD791_12745"
     CDS             6484..8583
                     /locus_tag="VD791_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXV08894.1"
                     /translation="MSELVTYAVQGHLGVITLNNPPVNALSVSKGVLQRILDAVKEGE
                     HDPAVRAFLLTGSGRAFSGGADISEFGKPTPMGVATLPVLAGYMDTVTKPIVAAIHGF
                     ALGGGLELALACHFRCAVAGTQLGLPEVKLGLLPGAHGTQRLPRLVGVERALDMIVSG
                     EPVTPERALELGLIDEIVKGDIAVAGAQFANRVVREGREIRRSSGLEAKLAQPADAFF
                     AAARARVAKEQRGYPAPLAIVDCLEAAVTLPFAKGAVRERELFERLRASGESKALRHL
                     FFAERQVAKIPDVPEDTATREIKSAAVIGAGTMGGGIAMNFANAGIPVKVLEMSQEAL
                     DKGLNVVRKNYAATVAKGRLKQEEMDRRMGLIQGVLRYDALKGADIVIEAVFEDMEVK
                     KQVFERLDAVAKPGAILATNTSTLDVNEIAAATRRPENVLGLHFFSPANVMKLLEVVR
                     ASATSKETLATAMKLARSIKKVGVVAGVCDGFIGNRMLHAYFREAGFLLEEGATPQQV
                     DRVIEDFGFAMGPFRVSDLAGLDVGWYIRKRQAATRPPHLRYSTVADRICEMGRFGQK
                     TGAGWYRYEAGNRTPLPDPEIEALIVSASKEAGIERRQVSDQEILERCIYALVNTGAQ
                     ILDEGIALRAADIDIVYIYGYGFPRYRGGPMFYADSVGLDQVYQAVQRFHAAHGQFWT
                     PAPLLERLARAGGAFNG"
     gene            8597..9775
                     /locus_tag="VD791_12750"
     CDS             8597..9775
                     /locus_tag="VD791_12750"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXV08895.1"
                     /translation="MTEAVIVSTARTPLCKSWKGALNLTHGATMGGHVVKHALERARL
                     EPGQVEDVIMGCANPEGATGWNIARQIALRAGMPVGIAGMTVNRFCSSGLQSVALAAQ
                     RILCGEGEIFVAGGVESISCVQNEMNKHMAQEQWLLENKPAIYWPMLQTAETVAKRYN
                     ISRERQDRYGVDSQLKAAKARAESKFRDEIAPIATTMKVVDKNTGAESLKQVTASEDE
                     GIRPDTTYEGVSNIKPAVEGGVIAAGNASQFSDGASAQVVMSDKAAAARGLRPLGVFR
                     GFAVAGCEPDEMGIGPVFAVPKLLQRAGLKVEDIDLWELNEAFAVQVIYCRDKLGIPN
                     ERLNVNGGSIAVGHPYGMTGSRLVGHALIEGKRRGARRVVVTMCIGGGMGAAGLFEVA
                     "
     gene            complement(9790..10116)
                     /locus_tag="VD791_12755"
     CDS             complement(9790..10116)
                     /locus_tag="VD791_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HXV08896.1"
                     /translation="MIHELRIYHCMPGRLPDLNKRFETATLAIWEKHGIRQAGFWTVL
                     VGPNNQSLYYLLEWDSMAERERKWGAFMADPEWLAKRAETERNGPLVERVENLFLAPT
                     AYSKVK"
     gene            10150..10827
                     /locus_tag="VD791_12760"
     CDS             10150..10827
                     /locus_tag="VD791_12760"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HXV08897.1"
                     /translation="MQRMCGRYELHTPVEEVARRFDAVLTDAARALPPRYNIAPTLQV
                     PVLRQGRRGREIEAMTWGLTPSWAKDRSGVKPINARAETVFERPMFRNAIARRRCLLP
                     ADGFYEWQRGPARKQPYHVGMADGGLLALAGVWEYWAQEGQEPLVSCCIVVTDANALM
                     AAIHERMPVIIAPQDYARWLDPALSGRATIEPMLAPLPAAEMRAYPISTRVNDVKNDA
                     PELIEPA"
     gene            10896..>11238
                     /locus_tag="VD791_12765"
     CDS             10896..>11238
                     /locus_tag="VD791_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08898.1"
                     /translation="MPPSQALSPRLATRWPALIGLAAVVLIGLGWWALVPEAERPAPL
                     PAREPVSAAEPAALAQQLLDGARTDLSRGNAPQARALYRRAVAEYERSGDQAGMAAAL
                     EEFAALEYGAGH"
BASE COUNT         1703 a         3752 c         3973 g         1810 t
ORIGIN      
        1 gcgaaagcgc ctgcggcgcg aggacaacgt cgcctgcgtg cccaccatgg gcaacatcca
       61 cgacggccat ctgtcgctgg tgcacatcgc gcgccgacat gctccgctga cggtgactac
      121 catcttcgtc aatcgcctgc agttcggcgc cggcgaggac ttcgacaagt acccgcgcac
      181 tttcgaggag gactgcgcca agctcgaggc ggccggaaac accatcgtgt tccatcccga
      241 cgagggcgag atgtatcccg agccgcaggc gttccatgtc gatccgccgg cgatcgccaa
      301 caagctcgag ggacgcttcc ggctgggtca tttccgcggc gtggccacgg tggtgctgaa
      361 gctcatcaac atcgtgcagc cgcagaccgc ggtgttcggc aagaaggatt accagcagct
      421 cgcgatcgtg cggcacatgg tggagcagct ggcgctgccg gtgaagatcg tgccggccga
      481 aaccgtgcgc gcgcccgacg ggctggcgct cagctcgcgc aaccgctacc tgagcgcggt
      541 cgagcgcgcg gaggcgccgc gcctgcagcg cgtgctgcag cgcgtgcgcg agcaggtggc
      601 gggcggcgag cgcaacttcg aggctatcga gttcgccgcc gacgccgagc tcgcgcgtca
      661 cggctggcac gtggactacg tggtggtacg cagccggcgc acgctgatgc agcccgagtc
      721 cggcgagacc gggctggtga ccctgggcgc cgcgaggctc ggcaatacgc gcctgatcga
      781 caatctcgag atcgacgcgc cgggctaacc gaggtcgcgg gctacacccg gcgccgtcgc
      841 ctaaaccccg ggcagccgat actcgcggta gtcttcgcgc agcctggtct tgagcagctt
      901 cccggtggcc gtgtgcggca gctcggcgac gaacaccacg tcgtccggga cccaccactt
      961 cgccaccttg ccctggaaga aggcgagcag ctcctcgcgc gtgagcgaag cgtcgggttt
     1021 cttcacgacg atcagcagcg ggcgctcgtc ccacttgggg tgcgccacgc ccaccaccgc
     1081 ggcttccttc accgcggggt gggccatcgc ggtgttctcc agatcgatcg agctgatcca
     1141 ttcgccgccg gacttgatca cgtccttgga tcgatcagtg atctgcatgt aaccgtccgg
     1201 gtcgatggtg gccacatccc cggtcgggaa ccagctcgcg ccgcactcgt cctgccgcag
     1261 gatagcgccg ccctcgccct tgaagtactg cgtggtgacc caggggccgc gcaccagcag
     1321 gtcgccgaag gccttgccgt cccagggcag cgctttgccc tggtcgtcca cgatctgcat
     1381 gtccacgccg aacagcggac ggccctgctt gtcttgtacc ttgagctgct ccgccttggg
     1441 cagcgccaga tgcttggcct tgagcgtgca caccgtgccc agcgggctca tctcggtcat
     1501 gccccaggcg tgcagcacgc gcacgccgta atcctgctcg aaagtgcgga tcatcgccgg
     1561 cgggcaggcc gagccgccga tcaccgtgcg gttgaaggtg ctgaacgcaa ggccgccttg
     1621 ctgcacgtgc tgcagcaggc ccagccacac cgtcggcacg ccggccgaca tggtcacctt
     1681 ctcccgctcg aacagctcgt agaggctggc gccgtcgagc tgcgcgccgg ggaacaccag
     1741 cttgctgccc gccagcgcgc aggcatacgg caatccccac gcgttgacgt ggaacatcgg
     1801 caccaccggc aggaccacgt cgcgcgccga caggttgagc gcatcgggca agcagcccgc
     1861 gtaggcgtgc agcacggtcg agcgatgggc gtacagcacg cctttcgggt tgccggtggt
     1921 gccggaggta tagcacagcc cggcagcggt ccattcgtcg aactgcggcc agtcgtagcc
     1981 atcggagtgg gcattgatca ggtcctcgta gcacagcagg ccgggcagcg gcagcttcgg
     2041 catgtgcgcg cggtcggtca tcgcgaccca gccgcgcacg cccgcgcaat gcggggcgag
     2101 cctctcgacc aatggcgcga aggtgagatc gaagcagacg tactggtcgt ccgcgtggtt
     2161 cacgatgtag gcaatctgct ccggaaacag gcgcgggttg acggtgtgga tcacggcgcc
     2221 catcccggat accgcgtagt acagctcgaa atgccggaag ccgttccagg cgagcgtgcc
     2281 cacccggtcg tgcatgcgca cgccgagcgc gcccagcgca ttggcgagct gacgcgcgcg
     2341 ccggtgcgca tcccggtagg tgtagcggtg tatgccccct tcgaccaggc gcgacacgat
     2401 ctcggtgtcg ccgtggtagc ggtccgcatg ccggatgagc gacgagatct gcaggggcgt
     2461 gttcatcatc aggccgttca tagggtgctc ctcgggcagc cgttgccggg catcgctggt
     2521 tctctttgcc gggcttcgcc gggattctag cagccgcacg cggccgctcc ggcgcggcgc
     2581 gcgcttccgg tgccggggcg gttgggtaga atggcgcttc cccgaccagg ccccgcgccc
     2641 ggcaccgccc ggcggcgccc gcttgcgcgc gcgcgcggga cgtcaacaac gacaatggag
     2701 ttcttcctcg tccagctgct caacagcctg tcctacgggc tgctgctgtt catgctgtcg
     2761 agcggcctga cgctgatctt cagcatgatg ggcgtgctca acttcgcgca cgccagcttc
     2821 tacatgctgg gggcgtatct cgccttccag ctctccagcc acctgggctt ctggccggcg
     2881 ctcgccctgg cgccgctgct ggtcggcgcg ctgggcgcgc tgaccgagcg ctggggattg
     2941 cgcaccgtgc acaagtacgg ccacgtggcc gagctgctgt tcaccttcgg cctgttctac
     3001 ctgatcgagg aggtggtgca cctcatgtgg gggcgtgccc cggtggacta ccgcatcccg
     3061 gcgtcgctcg atttcagcgc gttcaccctc ttcagcacca gctacccggc ctaccgcgta
     3121 ttcatgatgg cggtgtcggt aggcatgctg ctcgccctgt atctggtgct cacgcgcacc
     3181 cgcatcggca tgatcatcca ggcctccctg agccaccccg atatggtcga ggcgctcggc
     3241 cacaacgtgc cgcgcgtgtt catgctggtg ttcggcgggg gttgcgcgct ggccgggctg
     3301 gcgggtgtga tcgggggcaa cgccttcgtc accgaacccg gcatggccgc agcggtgggc
     3361 agcatcgtgt tcgtggtggt ggtgttcggc ggcatgggct cgctcaccgg cgccttcgtg
     3421 gcgtcgctgt tcttcggcgc cgtgcagacc ttctcggtgg cgctcgacta ctcgctgctc
     3481 gacctggccg gcgtgctggg cgtcgaggtc ggcgccgagt cggggtggcg ccggctgctg
     3541 tcgatcacga tcgcgcagac cgcgccggtg ctgccttacc tgctgatggt gctgatgctg
     3601 atcgtgcggc ccacagggct catggggacg agggagacgt gattcagcac cgcgtgaccg
     3661 aaaggcgtga ccgagaaccg tgaccgggaa gcgtgaccag attctgcgac gggcgttgct
     3721 gtggggcgcc acggcagggg tgatgatcgc gctgccgctg ctgttccggc agggcttcgc
     3781 cctgtcgatg ttgagccaga tgggcatcgc catcgtcttc gcactgtcct acaacatgct
     3841 gctcggccag accgggatgc tgtcgttcgg ccacgcggtg tactacggac tgggggcttt
     3901 cgtctgcgcg catacgctga acgcggtcgc ggacgggcgc ttcgcgctcc cggtcacgct
     3961 ggtaccgctg gtgggcggcg tgagcggcct cgccttcggc atcgtcttcg gctacgtcac
     4021 caccaagcgc tccggcacgc cgttcgcgat gatctcgctc ggcatcgccg agatggtggc
     4081 ggcctgcgcc ctgatgtttc ccggcttctt cggcggcgag ggcggcatcg ccaccaaccg
     4141 cgtgatgggc gagccgtggc acggcctgac ctacgggccg caggtgcagg tttactatct
     4201 gatcgccggg tactgcctgg cgtgcatgat cgccatgttc gcgctcaccc agacgccgct
     4261 cgggcgcatg gcgaacgcgg tgcgcgacaa cccggagcgg gtgcgcttcg tcggctacga
     4321 ccccaccgtc gtgcgctacc tggtgttctg cctggcgtcc ttcttcgccg gcatcgcggg
     4381 cggcctgggc accgtcaatt acgagatcgt gaccgccgag aacgtgggcc tgctggcctc
     4441 gggcaacgtg ctggtgatga ccttcgtcgg cggagtgggg tacttcttcg ggccagtgat
     4501 cggcgcggtg ctcgtgtcgt tcatgcaatc ggcgctgtcc agctacaccc aggcgtggct
     4561 gctctacttc gggctgttct tcgtggtgat ggtcctgttc gcgcccggcg gcatcgccag
     4621 cctgatcgtg atgcacggtc ccctgctgcg gcaccgactg tgggcgcggc tggtgcccgg
     4681 ctacctggcc gccggcgcgg cgttcgcggt gctgctcgcc gggctgatcg ggctggtcga
     4741 gatgatctat cacctcgagc gcggcgccga gtccggcgca gctttccggc tgttcgggca
     4801 ggcggtcgag gtagacctcc ccggtccctg gctcgccgcc ggcgccgggg tgctgctcgg
     4861 cctggcgctg ctgcgcccgg cgatcgccct ggcgcggcgc aattgggtga gtgtgcacgc
     4921 cgagttgcag cgggcgggag catgaacaac gccatcgagc tgcgcgccgt gcacaagagc
     4981 ttcgggcgca cggccatcat caacggcgtg gacctggcgg tggcgcccgg cgagcgccat
     5041 gcgatcatcg ggccgaatgg cgccggcaag tccaccctgt tcaacctgat cagcggccgc
     5101 ttccctgtca cctccggccg catcctgctc aacggcgagg acatcaccgg ccgcgagccg
     5161 caggagatca accaccgcgg cctggcgcgg agcttccaga tcaccaacat ctttccgcgc
     5221 ctctcggttt acgagaacgt gcgttgcgcg gtgctgcgct cgatgggcta cgggtattcc
     5281 ttctggcatc gggtgtcggc gctcgagcgc gtgcgccggc gcgcaggcga gatcgtcgag
     5341 cggatcgggc tcgcccgccg tcgcgaccgc gccgcggggg tgctgtccta tgccgagcag
     5401 cgcgcgctcg agatcggcat caccatcgcc ggcggggcag atgtgatcct gctcgacgag
     5461 cctaccgcgg gcatgagccg cagcgaaacg gccgacgcgg tggagctgat ccgcactgtg
     5521 accgggggca agaccctatt gatggtcgag cacgacatgg gggtggtgtt cggcctggcc
     5581 gaccgcatct cggtgctggt gtacggcaag gtgctcgcca ccggcacgcc gcaggagatc
     5641 cgcgccaacc ccgccgtgca ggaggcctat cttggcacgc aacattgaag aaaccgcaga
     5701 ggcgcgaagg cgcagagctt gagccatgct cgaagtgcga gacctgcacg cctactacgg
     5761 caagagccac atcctgcacg gggtggacat gacggtggga cagggcgaga tcgtctcgct
     5821 gctcgggcgc aacggcgccg ggcgctccac cactgtccgc gccgtcatgg gtcaggtggc
     5881 gaagagcggc tcggtgcgct tacgtggcga ggagatctcg gggctgcgca cctatctgat
     5941 cgcgcgccgc gggctgggct acgtgccgga gagccgcgag atattcccca cgctcacggt
     6001 gcgccagaac ctgatgctgg gcgcgaagac ccgcgcgcgc tcctcggcag gaagagccgg
     6061 ggggcgctgg tcgatggacg acctgttcgg cctgttcccg gtgctgcggg agcgtgccga
     6121 cgcgcccgcc ggcgtgatct ccggcggcga gcagcagatg ctcaccctgt gccgcacgct
     6181 gatgggcgac cccgacctgg tgatgatcga cgagcccacg gagggcctgg cgcccaaagt
     6241 cgtcgagctg gtcgcgcgcc tgttcgacga gatgaagggc cgcgggctgt cgatcctgct
     6301 ggtcgagcag aagctcacga tcgcgctgcg cctctcgcag cgcctgtacg tcatgggcca
     6361 cggccggatt gtgttccagg gcacgcccga tgacctgcgg gccaacgagc aggtgcgcaa
     6421 ggagtggctg gaggtctgac ctctgcgccc ctgtggttaa atagcgcttt cgaaggcacg
     6481 acaatgagcg aactcgtcac ctacgccgtg cagggtcacc tcggcgtgat cacgctgaac
     6541 aacccgccgg tgaacgcctt gagcgtcagc aagggcgtgc tgcaacggat cctggacgcc
     6601 gtcaaggagg gcgagcacga ccctgcggtg cgcgctttcc tgctgaccgg cagcggacgc
     6661 gcgttctccg gcggcgcgga tatctccgaa ttcggcaagc cgacgccgat gggcgtggcc
     6721 acactgccgg tgctcgccgg ctacatggac acggtgacca agcccatcgt cgccgccatc
     6781 cacggtttcg ccctgggcgg ggggctggag ctcgcgctcg cctgccattt ccgctgtgcg
     6841 gtcgccggca cccagctcgg cctgcccgag gtgaagctcg gcctgctgcc cggcgcccac
     6901 ggcacccagc gcctgccgcg cctggtgggg gtggagcggg cgctcgacat gatcgtgagc
     6961 ggcgagccgg taacgccgga gcgtgcgctc gagctggggc tgatcgacga gatcgtcaag
     7021 ggcgacatcg ccgtggccgg ggcgcagttc gccaaccgcg tggtgcgcga aggccgcgag
     7081 atccgtcgca gcagcgggct cgaggcgaag ctcgcgcagc cggcggatgc cttcttcgcg
     7141 gccgcccggg cgcgtgtggc caaggagcag cgcggctacc cggcaccgct ggccatcgtc
     7201 gattgcctgg aggccgccgt gaccctgccg ttcgccaagg gcgcggtgcg cgagcgcgag
     7261 ctgttcgagc gcctgcgcgc cagcggcgag tcgaaggccc tgcgccatct gttcttcgcc
     7321 gagcgccagg tggccaagat cccggacgtg cccgaggata ccgccacgcg cgagatcaag
     7381 tcggcggcgg tgatcggcgc gggcaccatg ggcgggggca tcgcgatgaa cttcgccaac
     7441 gccggcatcc cggtcaaggt gctggagatg agccaggagg cgctcgacaa aggcctcaac
     7501 gtggtgcgca agaactacgc ggccaccgtc gccaagggcc gtctcaagca ggaagagatg
     7561 gaccggcgca tgggcctgat ccagggcgtg ctgcgctacg acgcgctgaa gggcgccgac
     7621 atcgtcatcg aggcggtgtt cgaggacatg gaagtgaaga agcaggtgtt cgagcgcctg
     7681 gacgcggtgg cgaagcccgg cgcgatcctc gccaccaaca cctcgacgct ggacgtgaat
     7741 gagatcgccg ccgccacccg gcgtccggag aacgtgctgg gcctgcactt cttcagcccg
     7801 gccaacgtga tgaagctgct ggaggtggtg cgcgcgtcgg cgacctcgaa ggagacgctg
     7861 gccaccgcca tgaagctcgc gcgcagcatc aagaaggtcg gcgtggtggc cggcgtgtgc
     7921 gacggtttca tcggcaaccg catgctgcac gcatacttcc gcgaggccgg cttcctgctc
     7981 gaggaaggcg ccaccccgca gcaggtggac cgggtgatcg aggacttcgg cttcgccatg
     8041 ggaccgttcc gcgtgagcga cctcgccggc ctggacgttg gctggtacat ccgcaagcgc
     8101 caggccgcca cccgtccccc gcacctgcgc tactcgacgg tcgccgaccg gatctgcgag
     8161 atgggccgct tcgggcagaa gacgggtgcc ggctggtacc gctatgaagc gggcaatcgc
     8221 acgccgctgc ccgaccccga gatcgaggcg ctgatcgtga gcgcgtccaa ggaggcgggc
     8281 atcgagcggc gccaggtgag cgaccaggag atcctggagc gctgcatcta cgcgctggtg
     8341 aacaccggcg cgcagatcct cgacgagggc atcgcgctgc gcgcggccga catcgacatc
     8401 gtgtacatct acggctacgg ctttccgcgc tatcggggcg gccccatgtt ctacgccgac
     8461 agcgtcggcc tggaccaggt gtaccaggcg gtgcagcgct tccacgcggc gcacggccag
     8521 ttctggaccc ccgcgccgct gctcgagcgc ctggcgcgcg cgggcggtgc gttcaacggt
     8581 tgatttggag acgacaatga ccgaagcagt gatcgtatcg accgcgcgta cgcctctgtg
     8641 caagtcctgg aagggtgcgc tcaatctgac gcatggtgcg accatgggcg ggcacgtggt
     8701 gaagcacgcg ctcgagcgcg cgcggctcga acccggccag gtcgaagacg tcatcatggg
     8761 ctgcgccaat ccggagggag cgaccggctg gaacatcgcg cgccagatcg cgctgcgcgc
     8821 cggcatgccg gtcggcatcg cgggcatgac ggtgaaccgc ttctgctcct cggggctgca
     8881 gagcgtggcg ctggccgcgc agcgcatcct ctgcggcgag ggcgagatct tcgtggccgg
     8941 cggcgtggag tcgatctcct gcgtgcagaa cgagatgaac aagcacatgg cccaggagca
     9001 gtggctgctc gagaacaagc cggcgatcta ctggccgatg ctgcaaactg cagagaccgt
     9061 cgccaagcgc tacaacatct cgcgcgagcg gcaggaccgc tacggcgtgg acagccagct
     9121 caaggcggcg aaggcgcgcg cggagagcaa gttcagggac gagatcgcgc cgatcgcgac
     9181 caccatgaaa gtggtggaca agaacaccgg ggcggagtcg ttgaagcagg tcaccgcttc
     9241 cgaggacgaa ggcatccgcc ccgacaccac ctacgagggc gtgtccaaca tcaagccggc
     9301 ggtcgagggc ggtgtgatcg ccgcgggcaa cgccagccag ttttccgacg gcgcctcggc
     9361 ccaggtggtg atgagcgaca aggcggccgc cgcccggggg ctacggccgc tcggggtgtt
     9421 ccgcggcttc gccgtggccg gctgcgagcc ggacgagatg ggcatcggtc cggtgttcgc
     9481 cgtgcccaag ctgctgcagc gcgccggtct gaaggtcgag gacatcgacc tgtgggagtt
     9541 gaacgaagcg ttcgcggtgc aggtgatcta ctgccgcgac aagctcggca tccccaacga
     9601 gcggctgaac gtgaacggcg gctcgatcgc ggtgggccac ccctacggca tgacgggcag
     9661 ccgcctggtg gggcacgcgc tgatcgaggg caagcggcgc ggggcgaggc gcgtggtggt
     9721 gaccatgtgc atcggtgggg gcatgggcgc ggccggcctg ttcgaagtgg cctgagcgcg
     9781 cccaggcgat cacttcacct tcgagtacgc ggtcggggcc aggaacaggt tctcgacgcg
     9841 ctcgaccagc ggaccgttgc gctcggtctc cgcgcgcttg gcaagccact ccgggtcggc
     9901 catgaaggcg ccccacttgc gctcgcgctc ggccatgctg tcccattcca gcaggtagta
     9961 caggctctgg ttgttgggcc ccaccagcac ggtccagaag cccgcctggc ggatgccgtg
    10021 cttctcccag atagccagcg tggcggtctc gaaacgcttg ttcaggtccg gcaggcggcc
    10081 tggcatgcag tggtagatac gcagctcgtg gatcatgtct tgtcctccct cgaaagagcg
    10141 gatgctacca tgcagcgcat gtgcggccgc tacgagctcc acaccccggt cgaggaggtc
    10201 gcgcgccgct tcgacgccgt tctcaccgac gcggcgcgtg cgctgccgcc gcgctacaac
    10261 atcgcaccca cgctgcaggt gccggtgctg cggcagggca ggcgtgggcg cgagatcgag
    10321 gcgatgacct ggggcctgac gccgagctgg gccaaggatc gctcgggcgt gaaaccgatc
    10381 aatgcgcgcg ccgaaaccgt gttcgagcga cccatgttcc gcaatgccat cgcgcggcgc
    10441 cgttgcctgc tgccggccga cggcttctac gagtggcagc gggggccggc gcgcaagcag
    10501 ccctatcacg tcggcatggc cgacggcggc ctgctggcgt tggccggcgt gtgggagtat
    10561 tgggcgcagg aagggcaaga gccgctggtg agctgttgca tcgtggtgac cgacgccaac
    10621 gcgctgatgg ccgcgatcca cgagcgcatg ccggtgatca tcgcgccgca ggactacgcg
    10681 cgctggctcg acccggcgct ttccggacgg gcgaccatcg agcccatgct ggcgccgttg
    10741 ccggccgccg agatgcgcgc ttatccgatc tccacgcgcg tgaacgacgt gaagaacgat
    10801 gcgccggagc tgatcgagcc ggcgtgagcc gcggctgcgg tgcatgggca caagccgtgc
    10861 ttgcaccggt cttcgctttc gaattccgcg aacgcatgcc accctcacaa gccctctcgc
    10921 ccagattggc cacgcgctgg cctgcgctga tcgggctcgc cgctgtggtg ctgatcggac
    10981 tgggctggtg ggcccttgtc cccgaggccg agcggcccgc accgctgccg gcacgggagc
    11041 ccgtctccgc tgccgagccg gcagccctgg cgcagcagct gctcgacggc gcacgcacgg
    11101 atctgagccg gggcaacgcg ccgcaggcac gggcgctgta tcgccgagcc gtagccgagt
    11161 acgagcgcag cggcgaccag gccggaatgg cagcggcgct ggaggaattc gcagcgctgg
    11221 agtacggcgc gggtcatc
//
LOCUS       DASPGE010000392        12037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_104498, whole genome shotgun sequence.
ACCESSION   DASPGE010000392 DASPGE010000000
VERSION     DASPGE010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12037
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104498"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VD791_12770"
     CDS             <1..654
                     /locus_tag="VD791_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019719831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXV08899.1"
                     /translation="GQRCTTLRRLIVHRSLYDELLGRLQRLFQRVSIGDPLAAGTLVG
                     PLIDGRAYETMQQALSRARADAQGIAGGERCLEAEFPKAFYVRPALAAMRAQTEIMLE
                     ETFAPILYAVPYDTLDEAIVLHNAAQHGLSSAIFTNDLREAEAFLGAAGSDCGIANVN
                     IGTSGAEIGGAFGGEKDTGGGRESGSDAWKAYMRRATNTINYSRELPLAQGIRFDVS"
     gene            complement(768..2228)
                     /locus_tag="VD791_12775"
     CDS             complement(768..2228)
                     /locus_tag="VD791_12775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV08900.1"
                     /translation="MSPQPVQPQPPAPPPDPRRWLGLAGIGTGVFMFTLDASIVNVAL
                     PTLAQAFGASLAAVQWVPLAYLLVITTLVMGAARLGDVHGRKRAYLSGMMLFIAASAL
                     CGLAPSVGWLIAFRALQGLGGVFISALGAAIIAQIFPPGERGRALGVIASCVTLGVAL
                     GPTVGALIIDLAGWRWMFLVNIPVGIAALSIVARVVPDFEPTPARLPFDWLGTLLVAA
                     ALASFALALTFGQRQGFGAGLPLALFALALAALLAFVLAETRVDGPILELALFANPAF
                     SAGLAMSCLAFTVLGAVSFTLPFFLQLGLGLPIATVGLLMAISPVIGGIMGPIGGALS
                     DRFGPRWGAVVGLTLMTLGCILLMALDDSASIPRFALSVAPIGLGMGFFNSANNSAVL
                     NAVPRTRLGIASGLLSLARTLGQSTGVPVAASLLVIFALGHAEAVDHAALLDLPPISL
                     VRGTSGAFAAAAVAGFLGLCVAVWMLRYERAMRPKS"
     gene            2420..3715
                     /gene="hisD"
                     /locus_tag="VD791_12780"
     CDS             2420..3715
                     /gene="hisD"
                     /locus_tag="VD791_12780"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HXV08901.1"
                     /translation="MIRFLKTGISAERDAADLAQVRQTVEAILADIAQRGDEAVRAYS
                     RKFDNWDPPGFKLSDAEVQACIRAMSPREVDDIKFAQAQIRNFARIQRDALRDVEVET
                     LPGVILGHRNIPVNSVGCYVPGGKYPLVASAHMTILTAKVAGVKRVIGCAPPFQGRPA
                     PAIVTAMHLAGADEIFVLGGVQAIGAMAIGTVSMPACDMVAGPGNAYVAEAKRQLAGR
                     VGIDLFAGPTETLVIADDSCDGEMCATDLLGQAEHGPTSPAILLTNSEKLARATMAEV
                     EKQLRTLPTAAIAGQAWRDYGQVIVCDSYQEMVAEADRIASEHVQVMTRDPDYFLERM
                     TNYGALFLGPETNVSFGDKVIGTNHTLPTRKAARYTGGLWVGKFLKTCTYQKVKPEAS
                     AAIGEYCSRLCAIEGFAGHQAQADLRVRRYGHLKATAHR"
     gene            3747..4622
                     /locus_tag="VD791_12785"
     CDS             3747..4622
                     /locus_tag="VD791_12785"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV08902.1"
                     /translation="MAKLAFLGLGVMGYPMAGHLQKKGGHEVMVYNRTASKSAQWAKE
                     YGGRSAPTPREAAAGAEAVLMCLGNDDDVRSVVYGDSGALAGMKSGMILVDHTTDSAN
                     LARELHGKCKAAGIGFVDAPVSGGQAGAVNGQLGIMCGADAAVFARTKPVLDVYAKSC
                     VLIGEAGAGQLTKMVNQIAIAGLLQGLAEAINFGKRAGLDVEKVISVISKGAAQSWQM
                     ENRWKAVNEVKFDGFGFAVDWMRKDLGICLDEAKRNGARLPATALIDQFYAQVQARGG
                     GRWDTSSLMHLLAKD"
     gene            4626..5360
                     /locus_tag="VD791_12790"
     CDS             4626..5360
                     /locus_tag="VD791_12790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV08903.1"
                     /translation="MQGKVVVVTGGSGGIGSEICRHFAQAGASVVFTYRSGREAAQRL
                     QAALGPGHWARQVAVDDSAALGALAAEVESRHGRCEVLVNCAGITRFVPHADLDALDD
                     RLIDDIFRVNWRGAFAAVRAFRPLLRRGGAGLVVNISSIAGVTGMGSNVAYCASKAAL
                     DCMTKSLARALAPEIRVVSVSPGLVDTAFVKALDKTWRDEQAARTPLRRLATPDEVGA
                     AVLAVASVLEYSNGCIIPVDGGRPLT"
     gene            5388..6254
                     /locus_tag="VD791_12795"
     CDS             5388..6254
                     /locus_tag="VD791_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08904.1"
                     /translation="MTDVRRWASGLAGLCLSIAASAAAAAEPGSELIETIRAQRRQAI
                     ERVILQTTGEIESGQAEGKRLAEAFRGRGIARSYLLQYAEALQDFNQAIELDQVNPQY
                     YEDRAITYLKLREFGAASRDLDMALGLETKRSSAFREKGRLAFYQRDYDLAAREFARA
                     LDTASGAAVVYSAVWLHMAVKRGALSGEAPLAAILAQIDQGQWPVPVLQMFTGALQPA
                     EAVAAAVSSDPRSDLMLKCEGYFYAGQEHLIRADAKQAKAAFEAALATGVTEFLEYDW
                     AVRELELLKETP"
     gene            complement(6267..7622)
                     /locus_tag="VD791_12800"
     CDS             complement(6267..7622)
                     /locus_tag="VD791_12800"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 domain-containing protein"
                     /protein_id="HXV08905.1"
                     /translation="MRPLYACLLACAAAAAGAAEPRPEQFAYAVPVQIDGAQALQRLE
                     IPQSAYEGAARADLGDLRVFNGAGEPVPYAFMPRPAPGAEAAAPRPLRYFPLRGASGT
                     AAEALRIRVQRSADGTVVRVTIADGAPRESTATLVGYLVDATALKQPLAAIELDWRGA
                     DDLAGSLRVEGSDDLARWTTLAAAAPLVSLRFGGQRLEQKSVELPAVRYKYLRLSWPA
                     QQAPIELTALQGRPRDVQIEPERRWKTVTGRAAGEPGEHVFDLGGRLPVDRLRIALPQ
                     PNTLASVQVLARNGDDQPWGTICTGVLYRLQRDGAEIVNPDLAVAGSGWRFWLLRVDQ
                     KGGGIGQGEPTLEAGWVPQQLVFVARGDPPFQLAYGNAVAQPAAFPIATVVPGWRSDE
                     ELKATLATAGAQRELAGARALRAPIDTKTWALWGSLVLAVAVLGWMAWQLTRQMQPPK
                     T"
     gene            complement(7619..10351)
                     /locus_tag="VD791_12805"
     CDS             complement(7619..10351)
                     /locus_tag="VD791_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HXV08906.1"
                     /translation="MWFLGLMIGLVIGAVTEGGDGAVYGAAFGALAGYALRLALGRST
                     DARLDALEQDVRRLREALDAVRAAPPSQAVTAPRAEPFPEFELAAAPPGSMASIQIEP
                     EPAPPEAAPAPAAAAPSAPTPPRPPPSAPQAGSQPPAWWSWLVGGNTVVRVGVVVLFF
                     GVAFLLKYAYDHTHIPIQVRLIAVALGAVVLLVIGWRLRLRRRGYALALQGAGIGALY
                     LTVFASFRLFGLLPSGAALVLLFFIAALSAALAVLQSSQSLAILGASGGFLAPVLTST
                     GGGSHVMLFSYYALLNAGILGIAWYKAWRPLNLVGFAFTFGIGTLWGAQYYRPELFAS
                     TQPFLALFFALYLAIPVLYARREAGQTERYLDAVLVFGVPVIAFGLQLRLVRGFEYGG
                     AFSAVALSLVYLLLARTLHGREGERLRMLVEAFLALGVVFATLAIPLALDGRWTAAAW
                     ALEGAALVWVGVRQGRRAARAFGLLLQIGAGLAFLWDMPHATGRVPIFNSTYLGCLFV
                     ALAGLFSNWYLQRNRGALRNWEAVPAAAVFFWGLAWWVGGGLAEIQQQLSGRYEADAA
                     LLFFAGSAVALGWLCMRIGWRLALYPAFALLPVMALLAFGEAAFTWKRHPFAALGVIA
                     WPAAFAAHYWLLRRFEAPERSIPEVLHSGAVWLLAALGAWELAWNIDRAVLGEGIWPL
                     IAWVLFPGALLNLLSLWGQRIAWPVRAHLDSYLLLGCAPLAAFLYGWMLYANFTSNGN
                     PAPLPYVPLANPLDLAQIAALLVILLWLREARALQLLDLTGERMIAVMSALGTVVFVA
                     ANGVLLRSLHHYADVPFHLASLWRSMLVQAALSIFWSLLALCTMAIATRIRVRVLWIV
                     GATLMAVVVVKLFLVDLSGVSGVERIVSFIGVGLLMLVIGYLSPVPPRARELPQ"
     gene            complement(10442..11299)
                     /locus_tag="VD791_12810"
     CDS             complement(10442..11299)
                     /locus_tag="VD791_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HXV08907.1"
                     /translation="MRAFLLIGAAMAALALGAGSAAAAGVLTVGLIPSEDPRLMVNDN
                     RALLDHLRDTLQLEVKPYVATDYNGVIEALRAKKLDIALLGPFSYVLAVGVADVEAFA
                     VPETQNQGAGYHALIIARKDRNLRALKDLEGKTFAFVDPSSTSGHLFPKAALIKDGHD
                     PDRFFARVVFSGGHDASAIAVQNGKVDAAAIADALLEAAFKRGVAKREDIAVVWTSGR
                     IPGAPFVYRRDLPAALKARIRASFAQIRDMPWGTHNVIKRWEPTGDTAYDVVRDTARL
                     LNLDLKKMK"
     gene            complement(11383..>12037)
                     /locus_tag="VD791_12815"
     CDS             complement(11383..>12037)
                     /locus_tag="VD791_12815"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF011984.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HXV08908.1"
                     /translation="TPGQRQWMDVERFRVYSQRDQRWSDDHLAQVVEERQAMQARNAQ
                     RHRAGLLVLCRERRIPLASHDDTLPGHVEEALASGITICEFPTTLEAAHAARRHGLSI
                     VMGAPNLVRGGSHSGNVSALELARADLLDCLSSDYVPHALLHAAFLLRDLAGWSLPKA
                     LASTTSNPARMVGLGNRGRIAPGARADFVRVRETATAAQAGAVPVPLATWRGGQRIA"
BASE COUNT         1973 a         4196 c         4343 g         1525 t
ORIGIN      
        1 gggcagcgct gcaccacgtt gcgccggctg atcgtgcacc gcagcctgta cgacgagctg
       61 ctgggacgcc tgcagcggct cttccagcgc gtgagcatcg gcgaccctct ggcggccggc
      121 actctggtcg ggccactgat cgacggccgc gcgtacgaga cgatgcagca ggcgctgtcg
      181 cgcgcgcgcg ccgatgcgca ggggatcgca ggcggcgagc ggtgcctgga agccgagttt
      241 ccgaaggcgt tctacgtgcg tccggcgctc gcggcgatgc gcgcccagac cgagatcatg
      301 ctcgaggaga ccttcgcgcc gatcctgtac gccgtgccct acgacactct ggatgaggcc
      361 atcgtcctgc acaacgccgc gcagcacggc ctgtcgtcgg cgatcttcac caacgatctg
      421 cgcgaggccg aggcgttcct cggcgcagcc ggctccgact gcggtatcgc caacgtgaac
      481 atcggcacct ccggtgccga gatcggcggc gccttcggcg gggagaagga caccggcgga
      541 ggccgcgaat cgggctcgga cgcctggaag gcctacatgc ggcgcgccac caacaccatc
      601 aactactcgc gcgaactgcc gctcgcccag ggcattcgct tcgacgtgag ttgacccgcc
      661 aagcacgggt ggcgcgaccc gatgcgtagc ccggatggag cggagccaat ccggggacaa
      721 gaaaggcgct cgtctcgttt cccggattcc gctgcgctgc atccgggcta cgatttgggg
      781 cgcattgccc gctcgtagcg cagcatccac accgcgacgc acagaccgag gaagccggcg
      841 accgccgcgg cggcgaaggc gccgctggtg ccgcgcacca gcgagatcgg cggcagatcg
      901 agcagcgccg cgtggtccac ggcctcggcg tgaccgaggg cgaagatcac cagcagcgag
      961 gccgcgaccg gaaccccggt gctctgcccg agcgtgcgcg ccagcgacag cagtcccgaa
     1021 gcgatgccga gccgcgtgcg cgggacggcg ttcagcaccg cgctgttgtt ggccgagttg
     1081 aagaaaccca tacccaggcc gatgggcgcc acgctgagcg cgaagcgcgg gatcgaagcg
     1141 ctgtcgtcca gcgccatcag caggatgcag cccagcgtca tcagcgtcag gccgaccacc
     1201 gccccccagc gcggcccgaa gcgatcggac agcgcgccgc cgatcggccc catgatgccg
     1261 ccgatcaccg gcgagatcgc catcagcagg ccgacggtgg cgatcggcag ccccagtccg
     1321 agctgcagga agaacggcaa ggtgaagctg acggcgccca gcacggtgaa ggcgaggcag
     1381 ctcatcgcca ggccggcgga gaacgccggg ttggcgaaca gcgcgagctc aaggatcgga
     1441 ccgtccacgc gcgtttccgc gagcacgaag gcgagcagtg ccgccagcgc cagggcgaac
     1501 agcgcgagcg gcagccccgc gccgaagccc tgacgctggc cgaaagtgag cgccagagcg
     1561 aagctcgcca gcgccgccgc caccagcaac gtgcctagcc agtcgaaggg cagccgcgcg
     1621 ggcgtgggct cgaaatcggg caccacgcgt gcgacgatgg acagggcggc gatgcccacc
     1681 gggatgttga ccaggaacat ccagcgccaa ccggcgagat cgatgatgag cgcgcccacg
     1741 gtcgggccga gcgccacccc gagcgtgacg caggaggcga tcaccccgag cgcccgcccg
     1801 cgctccccag gcgggaagat ctgcgcgatg atggccgctc cgagcgccga gatgaacacg
     1861 ccgcccaggc cctgcagcgc gcggaacgcg atcagccagc ccacgctcgg ggcgagcccg
     1921 catagggccg aggcggcgat gaacagcatc atgccggaca ggtaggcgcg tttgcggccg
     1981 tgcacgtccc ccaggcgggc ggcgcccatg accagcgtgg tgatgacgag caggtaggcg
     2041 agcgggaccc actgcaccgc cgccagcgat gcgccgaacg cctgcgccag tgtcggcagc
     2101 gccacgttca cgatgctcgc atcgagcgtg aacatgaaca cccccgtgcc gattccggcc
     2161 aggccgagcc agcgcctggg atcgggcggc ggggcgggcg gttgcggctg aacgggctgt
     2221 gggctcatgg gcgcgtagcg cgaattcgcc gcgtgcggcg cgcggcgctc cggtggagcc
     2281 gcgatgctag ccgaggcgcg cggcgcgggc aaccgcgtta gcacccgcgt gcgcctcgca
     2341 cggcgcagca aggcgcggtc ggcggcccgg gcgtagaatc gcgcttctcg accgtccatc
     2401 ccgcagagcc gagcaaccca tgattcgatt cctcaagacc ggcatcagcg ccgaacgcga
     2461 cgccgccgac ctcgcgcagg tcaggcagac ggtggaggcg atcctcgccg acatcgcgca
     2521 gcgcggcgac gaagcggtgc gcgcctattc gcgcaagttc gacaactggg atccgcccgg
     2581 cttcaagctg tccgacgccg aggtccaggc ctgcatccgc gccatgtcgc cgcgcgaggt
     2641 ggacgacatc aagttcgccc aggcgcagat ccgcaacttc gcccggatcc agcgcgacgc
     2701 gctgcgcgat gtcgaggtgg aaacgctacc gggcgtgatc ctgggtcacc gcaacatccc
     2761 ggtgaacagc gtcggctgct acgtgccggg gggcaagtac ccgctggtgg cctccgcgca
     2821 catgaccatc ctcaccgcga aggtcgccgg cgtcaaacgc gtcatcggtt gcgcgccgcc
     2881 gttccagggc aggcccgcgc ccgccatcgt caccgccatg cacctggccg gcgccgacga
     2941 gatcttcgtg ctgggcggcg tgcaggcgat cggcgcgatg gcgatcggca ccgtcagcat
     3001 gccggcctgc gacatggtgg ccggccccgg caacgcctac gtggccgagg ccaagcgcca
     3061 gctcgccggg cgcgtgggca tcgacctgtt cgccgggccg accgagacgc tggtgatcgc
     3121 cgacgacagc tgcgacggcg agatgtgcgc gaccgacctg ctgggccagg ccgagcacgg
     3181 gcccacgtcg ccggcgatcc tgctcaccaa ctccgagaag ctcgcgcgcg ccaccatggc
     3241 cgaggtcgag aagcagttgc ggaccctgcc cacggcggcg atcgcgggcc aggcctggcg
     3301 cgactacggg caggtgatcg tgtgcgacag ctaccaggag atggtcgcgg aggccgaccg
     3361 catcgcctcc gagcacgtgc aggtgatgac ccgggacccg gactactttc tcgagcgcat
     3421 gaccaactac ggcgcgctgt tcctggggcc ggagaccaac gtgtcgttcg gcgacaaggt
     3481 gatcggcacc aaccatacct tgcccacgcg caaggcggcg cgctacaccg gcggcctgtg
     3541 ggtgggcaag tttctcaaga cctgcaccta tcagaaggtc aagcccgagg cctccgccgc
     3601 gatcggcgag tactgctcgc gcctgtgcgc gatcgagggt ttcgccggcc accaggcgca
     3661 ggccgacctg cgcgtgcggc gctacgggca cctgaaggcg acggcgcacc gctgaatttc
     3721 gacaccaacg acgaggacga cgcgacatgg caaagctggc attcctgggg ctgggcgtga
     3781 tggggtatcc catggccggg catctgcaga agaagggcgg tcatgaggta atggtgtaca
     3841 accgtacagc atcgaagtcg gcgcaatggg ccaaggagta cggcgggcgc agcgcgccca
     3901 cgccgcgcga ggcggcggcc ggcgccgagg cggtgctgat gtgcctcggg aacgacgacg
     3961 acgtgcgctc ggtggtctac ggcgacagcg gcgcgctggc cggaatgaag tccggcatga
     4021 tcctggtgga ccacaccacg gactcagcca acctggcccg cgagctgcac ggcaagtgca
     4081 aggcggcggg gatcgggttc gtggacgcgc ccgtatccgg cggccaggcc ggcgcggtga
     4141 acggccagct cggcatcatg tgcggcgcag acgccgccgt cttcgcgcgc accaagccgg
     4201 tgctcgacgt gtacgcgaag agctgcgtgc tgatcggcga ggccggtgcg gggcagctca
     4261 ccaagatggt gaaccagatc gccatcgccg gcctgctgca ggggctggcc gaggcgatca
     4321 acttcggcaa gagggccggg ctcgatgtcg agaaggtgat ctcggtgatc tccaagggcg
     4381 cggcgcagtc ctggcagatg gagaaccgct ggaaggcggt gaacgaagtg aagttcgacg
     4441 gcttcggctt cgccgtggac tggatgcgca aggacctcgg catctgcctg gacgaggcca
     4501 agcgcaacgg cgcgcgcctc cccgccaccg cactgatcga ccagttctac gcgcaagtgc
     4561 aggcgcgcgg cggcgggcgc tgggatacga gctcgctgat gcacctgctg gcgaaggatt
     4621 agcgggtgca gggaaaagtc gtcgtcgtca ccggcggctc gggcggcatc ggctccgaga
     4681 tctgccgcca cttcgcgcag gccggcgcga gcgtggtgtt cacctatcgc agcggccgcg
     4741 aggcggcgca gcggctgcag gcggcgctcg gccccgggca ctgggctcgg caggtggcgg
     4801 tggacgacag cgcggcgctg ggcgccctgg cggcggaggt cgagtcgcgc cacgggcgct
     4861 gcgaggtgct ggtgaactgc gccggcatca cgcgcttcgt gccgcatgcg gacctcgatg
     4921 cgctcgacga ccggctcatc gacgacatct tccgcgtgaa ctggcgcggg gcgttcgccg
     4981 cggtgcgcgc gttccggccg ctgctcaggc gcggcggcgc gggcctggtg gtgaacatct
     5041 cctcgattgc cggcgtcacc ggcatgggca gcaacgtcgc ctactgcgcg tcgaaagcgg
     5101 cgctggactg catgaccaag tctctcgcgc gcgcgctcgc gccggagatc cgtgtggtgt
     5161 cggtgtcgcc cggcttggtg gacaccgcgt tcgtgaaggc gctcgacaag acctggcgcg
     5221 acgagcaggc cgcgcgcacg ccgttgcggc ggctggctac gcccgacgaa gtgggcgcgg
     5281 ccgtgttggc ggtggcgagc gtgctcgagt acagcaacgg gtgcatcatt ccggtcgacg
     5341 ggggccggcc gctgacttga ccggcgggcg gaccgaagga ggcggcaatg actgacgtgc
     5401 gaaggtgggc gagcgggctg gcgggcctgt gtctgagcat cgcggcgtcc gccgccgcgg
     5461 cggcggaacc gggctcggag ctgatcgaaa cgatccgggc gcagcgccgc caggcgatcg
     5521 agcgcgtcat cctccagact accggcgaga tcgaatccgg tcaggccgag ggcaagcggc
     5581 tggcggaggc cttccgcggc cgcggcatcg cacgcagcta tctgctgcag tacgccgagg
     5641 cgctgcagga cttcaaccag gcgatcgagc tcgaccaggt gaatccgcag tactacgagg
     5701 accgcgccat cacctatctc aagctgcgcg agttcggtgc cgcttcgcgc gatctggaca
     5761 tggcgctcgg cctggagacc aagcgctcct ccgcgttccg cgagaagggc aggctcgcct
     5821 tctaccagcg cgactatgac ctggcggcgc gcgagttcgc gcgcgcgctc gacaccgcga
     5881 gcggtgcggc ggtggtgtac agcgcggtgt ggctgcacat ggcggtcaag cgcggcgcgc
     5941 tttcgggcga ggcgccgctc gccgcgatcc tggcgcagat cgatcagggc cagtggccgg
     6001 tgccggtgct gcagatgttc accggcgcgc tgcagccggc ggaggcggtc gcggcggccg
     6061 tttcgtccga cccgcgcagc gatctcatgc tcaagtgcga aggctacttc tatgccgggc
     6121 aggagcacct gatccgcgcc gacgcgaagc aggcgaaagc ggcgttcgag gcggcgctcg
     6181 ccaccggcgt caccgagttc ctcgagtacg actgggcggt gcgcgagctg gagctgctca
     6241 aggaaacgcc ctgagggcgc tcgccgctac gttttcggcg gctgcatctg gcgcgtgagc
     6301 tgccacgcca tccagccgag cacggccacc gccagcacca ggctgcccca cagcgcccag
     6361 gtcttggtgt cgatcggcgc acgcagcgcg cgcgcgccgg cgagctcgcg ctgcgcgccg
     6421 gcggtcgcca gcgtggcctt cagctcctcg tcgctgcgcc agcccggcac cacggtcgcg
     6481 atcggaaacg cggcgggctg cgcaacagcg ttcccgtaag cgagctggaa cggcgggtcg
     6541 ccgcgagcca cgaacacgag ctgttgcggc acccagccgg cctccagggt cggttccccc
     6601 tgcccgatgc cgccgccctt ctggtccacg cgcagcagcc agaagcgcca gccgctgccc
     6661 gcgaccgcga ggtcggggtt gacgatctcc gcgccgtcgc gctgcaggcg gtagagcacg
     6721 ccggtgcaga tcgttcccca cggctggtcg tcgccgttgc gcgcgagcac ctgcaccgag
     6781 gccagcgtgt tcggctgggg cagggcgatg cgcaggcggt ccaccgggag ccgcccgccc
     6841 agatcgaaca cgtgctcgcc cggctcgccc gcggcgcggc cggtgaccgt tttccagcgg
     6901 cgctcgggct cgatctgcac gtcgcggggc cgaccctgca gcgcggtgag ctcgatcggc
     6961 gcttgctgcg cgggccacga caggcgcagg tacttgtagc gcaccgcggg cagttccacc
     7021 gacttctgct ccaggcgctg cccgccgaag cgcaggctca ccagcggggc ggccgcagcg
     7081 agcgtggtcc agcgggccag atcgtcgctg ccttccacgc gcaggctgcc ggcgagatcg
     7141 tcggcgccgc gccagtccag ctcgatcgcg gccagcggct gcttcagcgc ggttgcgtct
     7201 accagatacc cgaccagcgt agcggtgctc tcgcgcggcg cgccgtcggc gatcgtcacg
     7261 cgcaccacgg tgccgtcggc gctgcgctgc acgcggatcc gcagagcctc ggcggcggtg
     7321 ccgctggcgc cccgcaacgg gaaatacctg agaggccggg gcgcggccgc ctcggcgccg
     7381 ggcgccgggc gcggcatgaa ggcgtagggc accggctcgc cggcgccgtt gaacacgcgc
     7441 agatcgccga ggtcggcgcg cgcggcgcct tcgtaggcgg actgcgggat ctccaggcgc
     7501 tgcagcgcct gcgcgccgtc gatctgcacc ggcacggcgt aggcgaactg ctccggccgc
     7561 ggctcggccg cgcccgcggc cgcggcggcg caggcgagca ggcaggcgta cagcggtctc
     7621 actgcggcag ctcccgggcg cgcggcggaa ccggcgacag atagccgatc accagcatca
     7681 gcaggcccac gccgatgaag gagacgatgc gctccacacc gctcacgccg gacaggtcca
     7741 ccaggaacag cttgaccacg accaccgcca tcagtgtcgc acccacgatc cacagcacgc
     7801 gcacgcggat gcgggtggcg atcgccatcg tgcacagcgc cagcaggctc cagaagatag
     7861 acagggccgc ctgcaccagc atcgagcgcc acagcgacgc caggtggaag ggcacgtccg
     7921 catagtggtg caggctgcgc agcagcaccc cgttggcggc cacgaacacc accgtgccca
     7981 gtgcgctcat gaccgcgatc atgcgctcgc cggtcaggtc gaggagctgc agcgcgcgtg
     8041 cctcgcgcag ccacagcaga atcaccagca gcgccgcgat ctgtgcgagg tcgagcgggt
     8101 tggcgagagg cacgtacggc aagggcgcgg ggttgccgtt gctggtgaag ttggcgtaca
     8161 gcatccagcc gtacaggaac gcggccagcg gcgcgcagcc cagcaacagg taggagtcga
     8221 gatgcgcgcg caccggccaa gcgatgcgct gtccccacag cgacagcagg ttgagcagtg
     8281 cgccggggaa caggacccac gcgatcaggg gccagatgcc ttcgcccagc actgcgcggt
     8341 cgatgttcca ggcgagctcc caggcgccga gcgcggcgag cagccacacc gcgccggagt
     8401 gcaggacttc tgggatggac cgttcgggcg cctcgaagcg ccgcagcagc cagtagtgcg
     8461 cggcgaacgc cgccggccag gcgatgacac ccagggcggc aaagggatgc ctcttccacg
     8521 tgaacgcggc ctcgccgaag gcgagcagcg ccatcacggg cagcagggcg aacgccgggt
     8581 agagcgccag gcgccagccg atgcgcatgc acagccaacc caatgcgacg gcggagccgg
     8641 cgaagaacag cagcgccgca tcggcctcgt agcggccgct cagctgctgc tggatctcgg
     8701 ccaggccgcc gcccacccac caggcgagcc cccagaagaa caccgccgcc gcgggcacgg
     8761 cttcccagtt gcgcagcgcg ccgcggttgc gctgcagata ccagttggag aacagtcccg
     8821 ccagcgccac gaacagacag cccaggtagg tgctgttgaa gatcggcacg cgccccgtgg
     8881 cgtgcggcat gtcccacaga aaagcgagac cggcgccgat ctgcagcagc agaccgaagg
     8941 cgcgcgcggc gcggcgcccc tgccgcacgc ccacccacac cagcgcggcg ccctccagcg
     9001 cccaggcggc cgcggtccag cgtccatcca gagccagcgg gatggcgagc gtggcgaaca
     9061 ccacgcccag cgcgagaaag gcctcgacca gcatgcgcag ccgctcgcct tcgcggccgt
     9121 gcagcgtgcg cgcaagcagc aggtagacca ggctcagcgc gaccgcgctg aacgccccgc
     9181 cgtattcgaa accgcgcacc aggcgcagct gcagcccgaa ggcgatcacc ggcacgccga
     9241 acaccagcac ggcatcgaga tagcgttcgg tctgcccggc ttcgcgtcgt gcgtacagga
     9301 cgggaatagc gaggtacagc gcgaagaaca gcgcgaggaa cggctgtgtg ctggcgaaca
     9361 gctcgggccg gtagtattgc gcgccccaca gcgtgccgat gccgaaggtg aaggcgaagc
     9421 cgaccaggtt gagcggtcgc caggccttgt accaggcgat gcccaggatg cccgcgttga
     9481 gcagcgcgta gtagctgaac agcatgacgt gactgccgcc gccggtggag gtgagcaccg
     9541 gagcgaggaa gccgccgctc gcgcccagga tggcgagcga ctgcgagctc tgcagcacgg
     9601 cgagcgcagc ggacagggct gcgatgaaaa acagcagcac cagcgcggca ccggagggca
     9661 acagtccgaa caggcggaag gacgcgaaca ccgtcagata cagcgcgccg atgccggcgc
     9721 cctgcagcgc cagcgcatag ccgcggcggc gcaggcgcag ccgccagccg atcacgagca
     9781 gcaccacggc gcccagcgca acggcgatga gccgcacctg gatcgggatg tgcgtgtggt
     9841 cgtacgcgta cttgagcagg aaggccacac cgaagaacag caccaccacg cccacgcgca
     9901 ccaccgtgtt gccgccgacc agccagctcc accaggccgg gggctgcgaa ccggcctgcg
     9961 gcgcggaggg cggcggtcgg ggtggggtgg gcgcggaagg cgcagctgcg gcgggcgcgg
    10021 gagcggcttc cggcggcgca ggctcgggtt cgatctggat ggaggccatc gaccccgggg
    10081 gcgcggcggc gagctcgaac tccgggaacg gctcggccct cggagcggtc acagcttgcg
    10141 acggcggcgc ggcacggacc gcgtcgagcg cttcgcgcag gcgccgcacg tcctgctcca
    10201 gcgcgtcgag gcgggcgtcg gtgctgcggc ccagcgccag gcgcagcgcg tagccggcga
    10261 gcgcgccgaa ggccgcgcca tacaccgcac cgtcgccgcc ttccgtgacg gcgccgatca
    10321 ccagcccgat catcagccca aggaaccaca tggtttgccc ctcgacctca accgcgcgct
    10381 gcgtaaacgg ctgactgatg caatcggcat gcctgcaacg gggcatgcca tgtcgcgccc
    10441 gttacttcat cttcttcagg tccagattga gcagccgggc ggtgtcgcgc accacgtcat
    10501 aggccgtatc gccggtcggc tcccagcgct tgatcacgtt gtgcgtgccc cagggcatgt
    10561 cgcggatctg cgcgaaggac gcccggatcc tcgccttcag agccgccggc aggtcgcgac
    10621 ggtagacgaa gggtgcgccc gggatgcgcc ccgaggtcca caccacggcg atgtcttcac
    10681 gcttcgcgac accgcgcttg aacgccgctt ccagcagcgc gtcggcgatt gccgcggcat
    10741 ccaccttgcc gttctgcacg gcgatcgcgc tggcgtcgtg gccgcccgag aagaccacgc
    10801 gcgcgaagaa gcggtcgggg tcatggccgt ccttgatcag cgcggccttg gggaacaggt
    10861 ggcccgaggt ggagctcgga tccacaaacg cgaaggtctt gccctcgaga tctttcagcg
    10921 cgcgcaggtt gcggtccttg cgcgcgatga tcagcgcgtg gtagccggca ccctggttct
    10981 gcgtttccgg cacggcgaag gcctccacgt cggcgacgcc gacggccagc acgtacgaga
    11041 acggtccgag cagcgcgatg tcgagcttct tggcgcgcag cgcctcgatc accccgttgt
    11101 aatcggtggc cacgtacggc ttcacctcga gctgcagggt gtcgcgcagg tgatcgagca
    11161 gcgcgcggtt gtcgttgacc atcaggcgcg ggtcctcgga cgggatcagc ccgacggtga
    11221 gcacacccgc cgcggcggcg ctgccggcgc cgagcgccag ggcggccatc gccgcaccga
    11281 ttaggagaaa ggctctcaag ctgctctccc tggaagagtc cgccgcaaac cgctgcgcct
    11341 gcccatcgtc cgttcgtgcc gaaccgatgg cgcgtgcgca tgttacgcga tgcgctggcc
    11401 cccgcgccag gttgccagcg gcaccggcac cgctccggcc tgagccgcgg tcgccgtctc
    11461 gcgcacgcgc acgaagtccg cgcgcgcccc cggtgcgatc cggccacgat tgccgagacc
    11521 gaccatgcgc gccgggttcg aggtggtgga ggcgagcgcc ttgggcaggc tccagccggc
    11581 gagatcgcgc agcaggaagg cggcgtgcag cagggcatgc ggcacgtaat cggaggacag
    11641 gcaatcgagc aggtcggcgc gcgccagctc gagcgccgag acgttgcccg aatgcgagcc
    11701 gccgcgcacc agattgggtg cgcccatgac gatgctcagg ccgtggcgcc tggctgcgtg
    11761 cgccgcctcg agcgtcgtcg ggaactcgca gatagtgatg ccgctcgcca gcgcctcttc
    11821 cacgtgcccg ggcagcgtgt cgtcgtggct ggcgagcggg atgcggcgtt cgcggcacag
    11881 caccagaagg ccggcgcgat gccgctgcgc gttgcgtgcc tgcatcgcct ggcgttcctc
    11941 gaccacctgc gcgaggtgat cgtcgctcca gcgctggtcg cgctggctgt acacgcggaa
    12001 gcgctccacg tccatccact ggcgctggcc gggcgtg
//
LOCUS       DASPGE010000393         7633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1039717, whole genome shotgun sequence.
ACCESSION   DASPGE010000393 DASPGE010000000
VERSION     DASPGE010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039717"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VD791_12820"
     CDS             complement(<1..1098)
                     /locus_tag="VD791_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXV08909.1"
                     /translation="MVNDINGLTVHLLEAGFQAEERPCIVLLHGFPELAYSWRKVMLP
                     IAAAGYHVIAPDQRGYGRTTGWDGDYDGDLDSFRVFNLVRDTLGLVAAFGYRSVAAVV
                     GHDFGSPVAAWCALLRPDVFRSAVLMSAPFPGPPSVPFDIVNMGNDKPRPGAAPGPTI
                     HDELAALARPRKHYHWYYSSREANANMRDCPQGVHAFLRAYYHYKSADWEQNRPFPLE
                     SWSAGELAKLPTYYIMDLDKGMAETVAPEMPSPAQIAACNWLPDDELSVYSAEYARTG
                     FQGGLQWYRCATSGRYIPELQIFSGRAIDVPSCFIAGKSDWGAYQRPGYLQSMQTSAC
                     TRMLGCHMVEGAGHWVQQEQPEAVCQLLVQFL"
     gene            1148..1753
                     /locus_tag="VD791_12825"
     CDS             1148..1753
                     /locus_tag="VD791_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08910.1"
                     /translation="MFIFEGGSCVVTGNATLSETGARQAGVSRCLGAAALGLALYIGG
                     VLSSGGVLSGAYAAGFELNGRALGDRMDEVLADQRYDCGGVSACFLLTACSFQAASAE
                     TVYGAPLTALTLHYLGERVAAIEARFSPARFDAVVAAALREHGPAQRDAQPAGAAGNA
                     VYTWRQGARLLRLERFFAGGESSLIIAQQSLLGELLAQPQE"
     gene            complement(1810..3885)
                     /locus_tag="VD791_12830"
     CDS             complement(1810..3885)
                     /locus_tag="VD791_12830"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXV08911.1"
                     /translation="MSVFDRIPLKPRLALTGFLPALAAVAATCALAVAGYHQETRVQL
                     LAEAAARAELTAKETVEPLQEEDARAARKSLDLLRLDPEVLSARLFDAYGKLVAEYRS
                     VELREAPPMSSPPRRALQRFHTAYLETAVPVMAGDAKYGTLVLLNDLHRSRYDVVAFG
                     VKLALIAIVALLLGCVAMMHLHGRIVAPLGELSDLVRHVLADKRYELRAHVHYADEVG
                     ALAKGFNAMLDRISDREATLHRELADRSQAQLRLEELAHFDPLTKLPNRHFFSRQLER
                     ALVDSARTGSAGALLLIDLHNFRAVNENFGHDAADSLLLQLSRRLTGRLREADMLCRL
                     GGDVFALVMEQLSGESHVVAVVNKILTALGEPIAVGDGEARLSACIGIAMFPVDGVEP
                     HQVLRHAQAALRRAKAAGGNMHCFFAPDMLDRTHPQQDIENELQRAVERGELRLHFQP
                     QVRLSDGELRGLEALLRWQHPERGLLLPGDFIPLAEENAGLVRAISDWTLNAVCTQIA
                     AWRAAGFEIVPVAVNYTPAQLRDPHMLSGLEALLKRYAVPGEYLELEITENLLMAEPR
                     AGETLVALRALGMRIAVDDFGTGYSSLAYLKELPITTLKIDRGFVHGLPGSNKYAAVI
                     RAIVGLATHLGFDTLAEGVESARQVDMLRAMGCTAYQGFCFSRAVPADEVERFLSGSA
                     QPVRRPALV"
     gene            4419..5387
                     /locus_tag="VD791_12835"
     CDS             4419..5387
                     /locus_tag="VD791_12835"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXV08912.1"
                     /translation="MSTELLRRAMSASNDGLVICDAATADMPIIYVNPAFERVTGYRA
                     EEVLGRNCRFLQRNDMRQPGLDPVREALRTGSNCVSMVRNYRKDGSMFWNELSIAPML
                     DASGRLTHFLGALSDATERVRTEHRQLEKQRQLEKSKRMLEGLALKDSLTGLYNRRHF
                     NEQLAREWNRARRDQLPLSLFMIDIDHFKRFNDTYGHLAGDHCIRLVGDTIRRCFARG
                     SDLVARYGGEEFVALAAGVERRQGRERAELLREAISELAGERHGRSGAHPVTVSIGLA
                     TATPDERISAEELLDAADRALYQAKRQGRDRVVQAPALRAALATAA"
     gene            complement(5400..6680)
                     /locus_tag="VD791_12840"
     CDS             complement(5400..6680)
                     /locus_tag="VD791_12840"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXV08913.1"
                     /translation="MPRHRTPYCAVLIAVSLAAAMPAQAESIRTGSGFAVAPGVVVTN
                     AHVVDGCRALRVVHGEQARDGRVLAIDREQDLAAVRTDLPVPATLPLRAAPALRLGES
                     VVAFGFPLTGSLSQEGNLTTGNVSALAGLRDDARHLQITAPVQPGNSGGPLLDEGGNV
                     IGVITAKLDAVAIAKRTGDIPQNVNFAVKGQELERFLRDARVTYETRVTDRQMAVADI
                     AEAVKRAAVRLECSSSGEPPSTQAARPSQSLDPVAPPSAGAPAMVEPAPALADAERAA
                     ILARLRVASVRTPYPTTSPGLRALTIVNDSSESVYKVTVGWLETPSARCPAAASAYRG
                     RKDVHVALAPGATRTTMSSFPADARLFCIVDAAFSPARSTQAAPGDGPPAENGPAQSE
                     PRGNEPTGTGQPAAADENDRPPAPPEENLPEENL"
     gene            6841..>7633
                     /locus_tag="VD791_12845"
     CDS             6841..>7633
                     /locus_tag="VD791_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV08914.1"
                     /translation="MFRNLVFRQLYDAVSSTYTYLLGDPDSRAAVVIDTVFEQHLRDL
                     ALIEELGLKLEAAIDTHCHADHVTGAWLMQQATGCHIGISRRYRPPVEGADLKLDHGD
                     RIAFGRRHLEVRATPGHTDGCLTLVLDDRSMAFTGDTLLIRGAGRCDFQQGNASLMYR
                     SITEQIFSLPDDCMLFPAHDYSGRTMSSVAEERAHNPRIGGHADERDFVGFMENLHLP
                     HPKQIDVALPANMRSGRPADGKVPRPAEWGPVRQSYAGLLEVDPEW"
BASE COUNT         1187 a         2608 c         2697 g         1141 t
ORIGIN      
        1 caggaactga acgagcaatt ggcacaccgc ctcgggctgt tcctgctgca cccagtgtcc
       61 ggcgccttcg accatgtggc agccgagcat gcgcgtgcag gcgctggtct gcatgctctg
      121 caggtagccg ggccgctggt aggcacccca gtcgctcttg cccgcgatga agcacgacgg
      181 cacgtcgatc gcgcggccgg agaagatctg caactcgggg atgtacctgc cgctggtcgc
      241 acagcgatac cattgcagcc cgccctgaaa gccggttctc gcgtattccg cgctgtagac
      301 gctcagctcg tcgtcgggga gccagttgca tgcggcgatc tgcgccggcg agggcatctc
      361 cggggccacg gtctccgcca tgcccttgtc cagatccatg atgtagtagg tcggcagctt
      421 cgccagctcg ccggcgctcc acgactcgag cggaaagggt cggttctgct cccagtcggc
      481 gctcttgtag tgatagtagg cgcgcaggaa ggcgtggacg ccctgcgggc aatcgcgcat
      541 gttcgcgttg gcctcgcgcg aggagtaata ccagtgataa tgcttgcgcg ggcgcgcgag
      601 tgccgccagc tcatcgtgga tggtaggccc cggcgcggcg cccggtcgcg gcttatcatt
      661 gcccatgttg acgatgtcga agggcacgga aggcgggccc gggaaggggg cgctcatcag
      721 caccgcggag cggaacacgt ccgggcgcag cagtgcgcac caggccgcga ccggcgagcc
      781 gaagtcgtga cccacgacgg ccgccacgga acggtagccg aacgccgcca ccagcccgag
      841 ggtgtcccgc accagattga aaacgcggaa ggagtcgagg tcgccgtcgt agtcgccgtc
      901 ccagcccgtg gtgcggccgt atcccctctg gtcgggggcg atcacgtgat acccggctgc
      961 ggcgatgggc agcatcacct tgcgccagct gtaggcgagc tccgggaagc cgtggagcag
     1021 gacgatgcag gggcgctcct cggcctggaa gcccgcttcg agcaggtgca cggtgaggcc
     1081 gttgatgtcg ttcaccaggc gggagcggat gccgtcgggc agcgcggtct tgccataggc
     1141 ggatgaagtg ttcatcttcg agggcggaag ctgcgtggtc acgggcaacg caaccttatc
     1201 agagaccggc gcacggcagg ccggcgtgag ccgctgcctc ggcgccgccg cgctgggcct
     1261 ggcgctgtac atcggcgggg tgctgtcgag cggcggcgtg ctgtcgggag cgtacgccgc
     1321 cggcttcgag ctgaatggcc gcgcgctggg cgaccgcatg gacgaggtgc tcgccgacca
     1381 gcgctacgac tgcggcggcg tatcggcgtg ttttctgctg accgcttgca gctttcaggc
     1441 ggcgagcgcc gagaccgtgt acggcgcacc gctcaccgca ttgacgctgc actacctcgg
     1501 cgagcgcgta gcggcgatcg aggccaggtt ttcgccggcc cggttcgatg cggtggtcgc
     1561 ggcggcgcta cgcgagcacg gcccggcgca gcgcgatgcg cagccggcag gcgcagcggg
     1621 caacgcggtc tatacctggc gccagggcgc gcggctgttg aggctcgagc ggtttttcgc
     1681 cggcggtgaa tcttcgctga tcatcgccca gcagagcctg ctgggcgagc tgctggcgca
     1741 gccgcaggaa tagcgggttt cgcagccggc ccgagtcaga cgcgtgctca gacgcgggcc
     1801 cggttctggt cagacgagcg ccggacggcg cacgggctgc gcagagccgg acaggaagcg
     1861 ttccacttcg tccgccggca ccgcgcggga gaaacagaac ccctgatagg cggtgcaacc
     1921 catggcgcgc aacatatcca cctggcgcgc cgactccacg ccctcggcca gggtatcgaa
     1981 gcccaggtgt gtggccagcc cgacgatggc gcggatcacg gcggcgtact tgttggagcc
     2041 cggcaggccg tgcacgaagc cgcggtcgat cttgagggtg gtgatcggca gctccttcaa
     2101 gtaggccagg ctcgagtagc cggtgccgaa gtcgtccacc gcgatgcgca tcccgagcgc
     2161 gcgcagcgcg accagggtct cgcccgcacg cggctcggcc atcagcaggt tctcggtgat
     2221 ctccagctcg agatactcgc cgggcacggc gtagcgcttg agcagcgcct ctaggccgga
     2281 cagcatgtgc ggatcgcgta gctgcgcggg cgtgtagttg accgccaccg ggacgatctc
     2341 gaagccggcc gcacgccagg cggcgatctg cgtgcacacc gcgttgagcg tccagtccga
     2401 gatggcgcgc accaggccgg cgttctcctc ggcgagcggg atgaaatctc cgggcagcag
     2461 caagccgcgc tcgggatgct gccagcgcaa cagcgcctcg agcccgcgca gctcgccgtc
     2521 cgacaggcgc acctgcggct ggaagtgcag gcgcagctca ccgcgctcga ccgcgcgctg
     2581 cagctcgttt tcgatgtcct gctgcggatg cgtgcggtcc aacatgtcgg gcgcgaagaa
     2641 gcagtgcatg ttgccgccgg cagctttggc acggcgcagg gcggcttgcg cgtgacgcag
     2701 cacctggtgc ggctcgacgc cgtccaccgg gaacatggcg atgccgatgc acgcgctcag
     2761 gcgcgcttcg ccgtcgccca ccgcgatcgg ctcgcccagc gcggtcagga tcttgttgac
     2821 cactgccacc acgtggctct cgccgctcaa ttgttccatc accagcgcga acacgtcgcc
     2881 gcccagccgg cacagcatgt cggcctcgcg caggcgtccg gtcaggcggc gcgagagctg
     2941 cagcagcaga ctgtcggcgg cgtcgtgccc gaagttctcg ttcaccgcgc ggaaattgtg
     3001 caggtcgatc agcaacagcg ccccggcgct gccggtgcgc gccgagtcca ccagcgcgcg
     3061 ctccagctgc ctggagaaga aatgccggtt cggcagcttg gtgaggggat cgaagtgcgc
     3121 cagctcctcg agccggagct gcgcctgcga gcggtcggcc agctcgcggt gcagcgtcgc
     3181 ctcgcggtcg gagatgcgat cgagcatcgc gttgaagccc tttgccaggg cgcccacctc
     3241 gtcggcgtag tgcacgtgcg cacgcagctc gtagcgtttg tcggccagca cgtgccgcac
     3301 caggtcggac agctcgccca gcggcgcgac gatcctgccg tgcaggtgca tcatcgcgac
     3361 gcagccgagc agcagcgcga cgatcgcgat caaggcgagc ttgacgccga aggcgaccac
     3421 gtcgtagcgg gaccggtgca gatcgttgag cagcaccagg gtgccgtact tcgcatcgcc
     3481 ggccatcacc ggcaccgccg tctccagata cgccgtgtgg aagcgctgca atgcgcggcg
     3541 cggcgggctg ctcatgggcg gcgcctcgcg cagctcgacg ctgcgatact cggccaccag
     3601 cttgccgtac gcatcgaaca gacgcgcgga cagcacctcc ggatcgaggc gcagcagatc
     3661 gaggctcttg cgggcagcgc gcgcgtcttc ctcttgcagc ggctcgaccg tttccttggc
     3721 ggtgagctcc gcgcgggcag cggcttcggc cagcagctgc acgcgcgttt cctggtgata
     3781 gccggcgacg gccagcgcac aggtggcggc gaccgcggcg agcgcgggca ggaaaccagt
     3841 cagcgccagc ctgggcttga gcggaatgcg atcgaacaca ctcatcggcg gcagttcctc
     3901 gtcgcgggag atggccgatc ggccgaaacg gaaacgcttg ccgcgcatcg ctcgatgccg
     3961 gtgacgtgtt ccgcctgctc gattctgctc agatagttca agccgtttcc ttccgggggg
     4021 cgcccgagtg cgactgtcgg gggggcctct gacaatccgt tgaccaatcg ggcgacgaca
     4081 ggagcacact ccgcaaccct tgtgccacgc gggcaacgtg gcactcggat gcggtcatgc
     4141 cacgcgcatc gccatgcgat ccgcgatgcg tgtgcgcggc gcgcgcgatg cccgcgccgg
     4201 caggcagccg ggcgcgcacg ccaagccgcg tctccggggg cgcagtgccg cgcccgagga
     4261 agggatgcaa catactgaaa acacgattga aaattcactt ccccgggctc gttctcaagg
     4321 ccgaaggtaa acaattgcta aagcttcggc cgcgcgggtc gaaactatag ccatcaaggg
     4381 cttctggttc cttgctgagg aatccaatga agagagatgt gagtaccgag ctgctgcgca
     4441 gggccatgtc tgcgtcgaac gacggactgg tgatctgcga cgcagccacg gcggacatgc
     4501 cgatcatcta cgtcaacccc gcgttcgagc gggtgaccgg ataccgggcc gaagaggtgc
     4561 tgggccgcaa ctgccgtttc ctgcagcgca acgacatgcg tcagccgggg ctcgacccgg
     4621 tgcgcgaagc gttgcgcacg ggatcgaact gcgtgtcgat ggtgcgcaac taccgcaagg
     4681 acggcagcat gttctggaac gagctgagca tcgcgccgat gctcgacgcc agcgggcgcc
     4741 tgacccactt cctcggcgcg ctgagcgacg ccaccgagcg cgtgcgcacc gagcaccgcc
     4801 agctcgagaa gcagcggcag ctggagaaga gcaagcgcat gctggaaggg ctggcgctca
     4861 aggacagctt gaccggcctg tacaaccgcc gccacttcaa cgagcagctc gcgcgtgaat
     4921 ggaatcgcgc gcgccgcgat cagctgccgc tgtcgctgtt catgatcgac atcgatcatt
     4981 tcaagcgctt caacgacacc tatggtcacc tcgccggcga ccattgcatc cgcctggtgg
     5041 gcgacaccat ccggcgctgc ttcgcgcgcg gctccgatct ggtggcgcgc tacggcggcg
     5101 aggagttcgt tgcgctagct gccggcgtgg agcggcgcca ggggcgcgaa cgcgccgagc
     5161 tgctgcgcga agccatcagc gagctggcgg gcgagcgcca tgggcgcagc ggggcgcatc
     5221 cggtcacagt gagcatcggg ctcgccaccg ccacgccgga cgagcgcatc tccgccgaag
     5281 agctgctgga tgcggcagac cgggcgctgt accaggccaa gcgccagggg cgcgaccgcg
     5341 tagtccaggc cccggcgctg cgcgccgcgc tcgccaccgc ggcgtaaccg gcaggcgccc
     5401 tacaggttct cttcgggcag gttctcttcg ggcggcgcgg gcggccgatc gttttcgtcg
     5461 gccgcggccg gctgcccggt cccggtcggc tcgttgcccc gcggctcgct ctgcgcgggt
     5521 ccgttttccg cggggggccc gtctccgggc gccgcctgcg tcgaacgtgc aggcgagaac
     5581 gccgcatcga cgatgcagaa cagccgcgcg tccgccggga agctactcat cgtggtcctg
     5641 gtcgcgccgg gcgccagcgc cacatgcacg tcctttcggc cacggtaggc ggatgcggcc
     5701 gcggggcagc gcgcggaagg cgtttccagc caccccacgg tcaccttgta cacgctctcg
     5761 gaggagtcgt tgacgatggt cagcgcgcgc agcccgggag aggtcgtggg ataaggcgtg
     5821 cgcaccgagg cgacgcgcaa gcgggcgagg atcgcggcgc gctcggcatc ggcgagcgcc
     5881 ggcgccggct ctaccatcgc cggcgcgccc gctgacggcg gggcaacggg atccagcgat
     5941 tgcgagggcc gcgctgcctg cgtggaaggc ggctcgccgc tcgacgagca ctccaggcgc
     6001 accgccgcgc gcttcacggc ctcggcgatg tcggccacgg ccatctgccg gtcggtcacg
     6061 cgcgtctcgt aggtcacgcg ggcatcccgc agaaagcgct ccagctcctg ccccttgacc
     6121 gcgaagttca cgttctgggg gatgtcgccg gtgcgcttgg cgatcgccac cgcatcgagc
     6181 ttcgcggtga tcacgccgat cacgttgccg ccttcgtcga gcagcggccc gccgctgttg
     6241 cccggctgca ccggcgcggt gatctgcagg tggcgcgcgt cgtcgcgcag gccggcgaga
     6301 gcgctgacgt tgccggtggt gagattgccc tcctgcgaca gcgatccggt cagcggaaag
     6361 ccgaacgcca ccacgctctc gcccagccgc agcgcgggcg ccgcgcgcag cgggagcgtg
     6421 gccggcaccg gcaggtcggt ccgcaccgcc gccaggtcct gctcgcgatc gatggccagc
     6481 acccggccgt cgcgcgcctg ctcgccgtgc acgacgcgca gtgcacgaca tccgtcgacg
     6541 acgtgcgcgt tggtgaccac tacgccgggc gccacggcga agcccgagcc ggtgcggatg
     6601 gattcggcct gcgccggcat cgcggccgcg agcgagacgg cgatgaggac ggcgcaatac
     6661 ggagtgcgat ggcggggcat gggggcaggc cgatatcggg tgggaacgct cagtgtaacg
     6721 ccggcgcggt cacgtcgttg accgggctgg gggagccccc ggcctgctgg gcggccgcgc
     6781 gcagccacgc gcaagcgtca gtcgtggtgt aattggcgaa tcgctccgcc cggagtcccc
     6841 atgttccgca acctcgtctt ccgtcaactc tacgacgccg tgtcgtccac gtacacctat
     6901 ctgctggggg accccgattc gcgcgccgcg gtggtcatcg acacggtgtt cgagcagcac
     6961 ctgcgcgatc tcgccctgat cgaggagctc ggcctgaagc tcgaagccgc gatcgacacc
     7021 cactgccacg ccgaccacgt caccggcgcc tggctgatgc agcaggccac cggctgccac
     7081 atcggcatct cgcggcgcta ccggccgccg gtcgagggcg ccgatctgaa gctcgaccac
     7141 ggcgaccgca tcgccttcgg ccgccggcat ctcgaggtgc gcgccacgcc gggccacacc
     7201 gacggctgct tgaccctggt gctggacgat cgcagcatgg cgttcaccgg cgacacgctg
     7261 ctgatccgcg gcgccggccg ctgcgacttc cagcaaggca acgccagcct gatgtaccgc
     7321 tcgatcaccg agcagatctt ctcgctgccc gacgattgca tgctgttccc ggctcacgat
     7381 tacagcgggc gcaccatgtc ctcggtggcc gaggagcggg cgcacaatcc gcgcatcggc
     7441 ggccacgccg acgagcgcga cttcgtcggc ttcatggaga acctccacct gccgcacccc
     7501 aagcagatcg acgtcgcgct gccggccaac atgcgttccg ggcggccggc ggacggcaag
     7561 gtgcccaggc cggcggaatg ggggccggtg cggcagagct atgccggcct gctcgaggtg
     7621 gaccccgagt ggg
//
LOCUS       DASPGE010000394        14215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1744604, whole genome shotgun sequence.
ACCESSION   DASPGE010000394 DASPGE010000000
VERSION     DASPGE010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14215
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744604"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VD791_12850"
     CDS             complement(<1..588)
                     /locus_tag="VD791_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HXV08915.1"
                     /translation="MAGDDALLDEFLDSLWLEDGLSRNTLDSYRRDLRQFAAWLQARS
                     RRGLLEAQHADLLDYLGHRYQQRAKASSTARLLSSLKRFYRHALRQGKLGADPTLNID
                     APRLGRRLPKSLTEADVEALLAAPDVDTPLGLRDKAMLEMLYASGLRVSELVSLTAAQ
                     LSQEMGVVRILGKGSKERLVPVGEEALDWVRRYGAQ"
     gene            complement(596..1075)
                     /locus_tag="VD791_12855"
     CDS             complement(596..1075)
                     /locus_tag="VD791_12855"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HXV08916.1"
                     /translation="MLETYSARIAAPFAVLGIRTTGEWLTGIDYLPLGAPVLAPLDAF
                     SAAVCRQLEAYLRDPDFRFDLPCRNPGSGYQQRVWGAIQRIPRGQTMSYAEVAGRIGS
                     APRPVGMACGANRVPLLIPCHRVVASGGIGGFMHSRGGRAIEIKRWLLRHEGVRLLS"
     gene            complement(1099..1488)
                     /gene="rplS"
                     /locus_tag="VD791_12860"
     CDS             complement(1099..1488)
                     /gene="rplS"
                     /locus_tag="VD791_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HXV08917.1"
                     /translation="MDLIAKIEQEEIARLGKSVPPFAAGDTVVVNVNVVEGDRKRVQA
                     YEGVVIAKRNRGVHSSFIVRKISSGEGVERTFQTYSPLIASIEVKRRGDVRRAKLYYL
                     RARSGKSARIREKLSAAEKAGAIEAED"
     gene            complement(1509..2285)
                     /gene="trmD"
                     /locus_tag="VD791_12865"
     CDS             complement(1509..2285)
                     /gene="trmD"
                     /locus_tag="VD791_12865"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HXV08918.1"
                     /translation="MIHFDAVTLFPEMFDAITRSGVTRRAFENGIYELVLWNPRDFSS
                     NAYRSVDDRPYGGGPGMVMLSEPLEKALKAARQRQRSCGVKESRVIYFTPQGSPLTHA
                     RVGELLQGDGLILLCGRYEGVDERLILRQVDCEVSIGDYVLSGGELPAMVLMDAMIRQ
                     LPGVLGDEASAAQESFVGGLLDHPHYTRPEVYEGMPVPPVLLSGNHAQIERWRMKQAL
                     GRTWSRRPELLARVVLTTEQRALLEEYQREAQQAAPAQSG"
     gene            complement(2282..2794)
                     /gene="rimM"
                     /locus_tag="VD791_12870"
     CDS             complement(2282..2794)
                     /gene="rimM"
                     /locus_tag="VD791_12870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HXV08919.1"
                     /translation="MGTLPPEAMIIMGRVAAPFAVSGWVRIQVFTERPDGLLGYPDWW
                     LRRDGQWRQAMVEEAAVHGRVVVAKLKGCDDREAAAALRGCEVAVPRAALPRNAEGEY
                     YWADLEGLRVRNLQDQDLGRIAGLIDTGANRVLVVRGERERLIPFVGAVVQSVDLARG
                     EVAVDWGADW"
     gene            complement(2832..3101)
                     /gene="rpsP"
                     /locus_tag="VD791_12875"
     CDS             complement(2832..3101)
                     /gene="rpsP"
                     /locus_tag="VD791_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HXV08920.1"
                     /translation="MVVIRLARGGAKKRPFFNVVVADSRNPRDGRFIERVGFYDPKAP
                     AGRESLRLDLQRLAHWQGKGAKLSDTVARLVRQFGKAAQPQAASA"
     gene            complement(3171..3953)
                     /locus_tag="VD791_12880"
     CDS             complement(3171..3953)
                     /locus_tag="VD791_12880"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXV08921.1"
                     /translation="MKTLHRMLLIALALSGPMPSAWGISDYDREKRWADQIVPSLMVG
                     EAVWIQQAGGHKFLALYAEAEKPRGALIVAHGRGWSPDYELYGMLRTKLAERGYTTLA
                     IQLPVLGGGAKIGDYLATYPDARERFDLAAKYLLARGYKNIGIVSHSLGAAMANQYLI
                     NVDQTPVKAWAFVGIINGLEEMFRIKIPVLDVFGSKDWEITQVGAYERKRQIDKVPGS
                     EQIVVKDALHFFEGREDELVQILTSFMDRVFHGGDPAAASSR"
     gene            complement(4032..5381)
                     /gene="ffh"
                     /locus_tag="VD791_12885"
     CDS             complement(4032..5381)
                     /gene="ffh"
                     /locus_tag="VD791_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HXV08922.1"
                     /translation="MLESLTGRLSGVIKSLRGQARLTESNIQDALREVRVALLEADVA
                     LPVVKDFIAHVKERALGREVAGSLTPGQALVGVVHDELTALMGAANAPLHLSVVPPAV
                     ILMAGLQGSGKTTSAGKLAKWLHEDLRKKVLLVSCDVYRPAAIEQLQTLARQVDVEFY
                     PVSAASDPVDIARAAHDYARKHYHDVMIVDTAGRLAVDQAMMDEIRALHAALEPAETL
                     FVVDAMQGQDAVNVANAFGAALPLTGIVLTKLDGDARGGATLSVRHVTGKPVKLVGVG
                     EKLNALEPFHPERMASRILGMGDVLSLVDEARRTVDLEQAEKLAKKVKSGKGFDLEDF
                     KQQMLQMRKMGGLTALMDKLPAQLTQMAQGANTDEKVVARTIGIIDSMTPAERARPEL
                     IKASRKRRIAGGAGVPVQEVNRLLNQFEQMQKMMKMMSKGGMQKMMRNLRGMLPGMR"
     gene            5521..6309
                     /gene="ccsA"
                     /locus_tag="VD791_12890"
     CDS             5521..6309
                     /gene="ccsA"
                     /locus_tag="VD791_12890"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HXV08923.1"
                     /translation="MCAALYAAAAVLLYRSLAGRDRPGWELGALHWATLAPLLLHTWL
                     LYRMVFHPDGIYLGVGTSVSVIIWLTVAIYWLGSFVYRLHGLQVLILGAAAGLVLLPA
                     LLPSVRPLSHANLAAFRLHLLISLLAYSLFTIASLQALMMAVLERRLHGGALPAYLQA
                     LPPLLTLERLLFRIITAGFLLLTLTLASGMLFSEALLGHPLPFTHKVIFGILAWLIYA
                     ALLAGRRLYGWRGRTALRWTLGGFLALVLAYIGSKFVLEVILQR"
     gene            6417..7691
                     /locus_tag="VD791_12895"
     CDS             6417..7691
                     /locus_tag="VD791_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HXV08924.1"
                     /translation="MDEIPLHTLLAAFAILLLISAFFSISETSMMALNRYRLKHLART
                     GHRGARLVAQLLERTDKLLGVILLGNNLVNTAAAALGTVIAFRAMGESELAVSIATGA
                     VAFFLLVFSEVTPKVVGATYPERVAFPAAFVLTPLLKLAYPLVWLVNGLAGGLLWLLR
                     IRRQPAAQGAVPLSSEELRTLVLEGGHYLPTKHRKILLNLFELQSITVADLMTPRSRL
                     ETLDLDQPPEALRRQIASSNHTRLPVYRETLDNIAGVLHVRKVLNLDARQVDAQALSE
                     VMRPAYFVPMGTTLLAQLQNFQEEEERFGLVVDEYGELMGLITLEDILEEIIGEFTTP
                     SPLTAGAVLRQDDGSYIVEGRIPLRELNRELELQLPLEGPKTLNGLILERLEDIPEPG
                     TVVKIAGYPIEILQTQHRAIRSARIFPTERAD"
     gene            7810..9525
                     /gene="pilB"
                     /locus_tag="VD791_12900"
     CDS             7810..9525
                     /gene="pilB"
                     /locus_tag="VD791_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HXV08925.1"
                     /translation="MVAASTENQLSGLGRAMVQKGLLAEREATALQHKANEAGVTFVE
                     QLVGSKRFGEVEVAEFAAQTFGFPLLDLQAFDLDHLPSKHIDPKVLGGRRALPLFQRG
                     NRLFVAISDPTNRAAMDQIKFQTGLHIEPVVVEDTKLGILIGKLVAASGAALSELVAE
                     DMAAELTEADPEPQADSQSIEIDDAPVVKYIQKIMLDAIDSGASDIHFEPYEKVYRIR
                     YRLDGELYEVANPPLAIKEKIASRIKVISRLDISEKRVPQDGRMKLVLSKTRAIDFRV
                     STLPTLFGEKLVLRILDPSSASLGIDALGYDPDQREALLNAVHRPYGMVLVTGPTGSG
                     KTVSLYTCLNILNQPDINIATAEDPAEINLPGINQVNVNERAGLTFAVALRAFLRQDP
                     DVIMVGEIRDLETGEIAVKAAQTGHLVLSTVHTNDAPSTLTRLMNMGIAPFNIASSVI
                     LITAQRLARRLCSHCKRPADVPKEALLRAGFSERDLDGRWTPYAHVGCEHCKNTGYKG
                     RVGVYQVMPMSEAINHIILTSGNAMELAAQARREGVRDLRQSGLLKVRQGVTSLEEVE
                     AVTNE"
     gene            9547..10779
                     /locus_tag="VD791_12905"
     CDS             9547..10779
                     /locus_tag="VD791_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXV08926.1"
                     /translation="MATAQATSAARRDVKEFMFAWEGRDRGGKLVKGDMRASGEAVVQ
                     STLRRQGIHVLKIRRQRGGVGGKITEKDITLFTRQLATMMKSGVPLLQSFDIVGKGHS
                     NPAVGKLLLDIKTDVETGSSLNQAFRKYPLHFDALFCNLVAAGEAAGILDNLLDRLAS
                     YKEKILAIKSKIKSALFYPIAIIVVAFLITAVIMIFVIPAFKELFTNFGADLPAPTVM
                     VMNLSDIFVTYWYLIFGTIGGGLWFFFYTWKRSAKMQAAMDRAFLKLPVFGDIIRKAT
                     MARWSRTLATMFAAGVPLVEALDSVGGASGNYVYLIATKKIQQEVSTGTSLTVSMQNS
                     EVFPNMVIQMVAIGEESGSLDAMLSKVADFYEQEVDDAVEALSSLMEPMIMVVLGTLI
                     GGLVIAMYLPIFKMGQAI"
     gene            10888..11817
                     /locus_tag="VD791_12910"
     CDS             10888..11817
                     /locus_tag="VD791_12910"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HXV08927.1"
                     /translation="MSTLQLLAHSPDLFGGVCLLLGLLVGSFLNVVIHRLPVMLRRDW
                     RRQSVEILSEWSEEKEIPGELAQMRAGLEKLGPALAAARAYNLIVPRSACPRCGHRIG
                     ALQNIPVVSYLVLRGRCAGCNARISPRYPVIEAFTGLVSACVGWHFGFSLAALAGLLF
                     AWALIAAAVIDFDTQLLPDDITLPLTWLGLSLNLWGALTTLESAVIGAVAGYLALWSV
                     YWVFKLTTGKEGMGFGDFKLLAAIGAFLGWKMLPAVILLSSLVGAVVGIGLIALAKRG
                     RNVPIPFGPYLAAAGLLALFFGEDLNRAYLQLL"
     gene            11817..12416
                     /gene="coaE"
                     /locus_tag="VD791_12915"
     CDS             11817..12416
                     /gene="coaE"
                     /locus_tag="VD791_12915"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014722400.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HXV08928.1"
                     /translation="MLVVGLTGGIGSGKSAVAALFQALGAAVIDTDVIAHELTAPGQP
                     AVEQIRARFGAHYVTAQGALDRARMRELVFADAAAKRDLEAILHPRIREQAAARVAQA
                     GTPYAILVVPLLAETGGWPGLIDRVLVVDCEESLQTERTRRRSGLSADQVQAIMARQA
                     TRAQRLALADDVIDNSGPVEALEEQVRRLHARYLSLAQA"
     gene            12538..13293
                     /gene="zapD"
                     /locus_tag="VD791_12920"
     CDS             12538..13293
                     /gene="zapD"
                     /locus_tag="VD791_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HXV08929.1"
                     /translation="MISYEFPLNERIRTLLRMESLYERLRFFVEHDERLEHHAALLTL
                     FEFLEVAGRADLKSEVIQELERQRQSLSALRSNPAISERALSEVLDDIDRAVQGMQQS
                     SGKTGQELRENEWLMGIKQRAAIPGGVCEFDLPSYHHWLHQPAADRRRDLESWITPFL
                     PLQQGVGIILRILRDSGRTGQQLAAQGVYQQMMGGRLAQLLRLRVDDAYDCVPEVSAN
                     KYALNIRFTVGSGQQRPRVSQTDIPFELTFCNL"
     gene            13293..13508
                     /locus_tag="VD791_12925"
     CDS             13293..13508
                     /locus_tag="VD791_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011544509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HXV08930.1"
                     /translation="MAEDRRKVVICPQCGAPTPWSELNPHRPFCSERCKLIDLGQWAS
                     EGYRIPVAQDESVSGDETVTEKRDRTA"
     gene            complement(13698..>14215)
                     /locus_tag="VD791_12930"
     CDS             complement(13698..>14215)
                     /locus_tag="VD791_12930"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HXV08931.1"
                     /translation="LEPFLRQLDAALARGLRMVMVREKKMRADALAVFAGEVVRRCHA
                     AGAQALIHGDARLAAACGADGVHLSAPALLQASTRPSAGLCGASCHDAGELQQAQRLD
                     LDYAVLGAVLPTASHPGQPALGWERFAELIADCTLPVYALGGMQPRHLERAWLAGAHG
                     IAMMRAAWETA"
BASE COUNT         2201 a         4989 c         4693 g         2332 t
ORIGIN      
        1 ctgcgcgccg tagcggcgca cccagtcgag cgcctcctcg cccaccggta ccaggcgttc
       61 cttggagcct ttgcccagga tgcgcaccac gcccatttcc tgcgacaact gggccgcggt
      121 gagcgacacc agctcggaca cgcgcagtcc cgaggcatac agcatctcca gcatggcctt
      181 gtcgcgcagc cccagcggcg tgtccacgtc gggcgccgcg agcagcgcct cgacgtcggc
      241 ctcggtcagg ctcttgggca gccggcgccc gagccgggga gcgtcgatgt tgagggtggg
      301 gtcggcgccg agcttcccct ggcgcagcgc gtggcgatag aaacgcttca ggctcgacag
      361 caggcgcgcg gtggagctgg ccttggcgcg ttgctggtag cggtgtccga ggtagtcgag
      421 cagatcggca tgctgcgctt cgagcagacc gcggcggctg cgcgcctgca accaggccgc
      481 gaactgacgc aggtcgcgcc ggtagctgtc cagggtgttg cgcgacagcc cgtcctcgag
      541 ccacaggctg tcgagaaatt cgtcgagcag cgcgtcgtcg ccggccaccg cgcccctagg
      601 aaagcagccg cacgccctcg tgccgcagca gccagcgctt gatctcgatg gcgcgccctc
      661 cgcggctgtg catgaatccg ccgatcccgc cggaagccac cacgcggtgg caggggatca
      721 gcagcggaac gcgattggcg ccgcaggcca tgcccaccgg gcgcggcgcg gagccgatgc
      781 gcccggccac ttcggcgtag ctcatggtct gcccgcgcgg gatgcgttgg atggcgcccc
      841 acacgcgctg ctggtaaccg ctgcccgggt tgcgacaggg taagtcgaag cggaagtcgg
      901 gatcccgcag atacgcctcg agctggcggc acactgcggc ggagaaggcg tcgagcggcg
      961 cgagaacggg cgcgccgagc ggcaggtaat cgattccggt gagccactcc ccggtggtgc
     1021 ggatgccgag caccgcgaac ggtgcagcga tacgggcgct gtaggtttcc agcatctcac
     1081 gccgcgccgg ccgccgcctc agtcctccgc ctcgatcgcg ccggcctttt cggcggcgga
     1141 cagcttctcg cggatgcgcg ccgacttgcc cgagcgggcg cgcaggtaat acagcttggc
     1201 gcggcgcacg tcgccgcgac gtttcacctc gatgctcgcg atcagcggcg agtaggtctg
     1261 gaaggtgcgc tccacgccct cgcccgagga gatcttgcgc acgatgaagg acgaatgcac
     1321 gccgcggttg cgcttggcga tcaccacgcc ctcgtaggcc tgcacgcgct tgcggtcgcc
     1381 ttccacgacg ttgacattca ccaccaccgt gtcgcccgcg gcaaagggcg gcaccgactt
     1441 gcccaggcgg gcgatctctt cctgttcgat cttcgcgatc agatccatgt ttgcacgctc
     1501 ctcacatctc atccgctctg cgccggcgcg gcctgctgcg cctcgcgctg gtattcctcg
     1561 agcagcgccc gctgctcggt ggtgagcacc acgcgggcga gcagctccgg acggcggctc
     1621 caggtgcggc ccagcgcctg cttcatgcgc cagcgctcga tctgcgcgtg gttgcccgac
     1681 agcagcaccg gcggcaccgg catgccttcg tacacctccg ggcgcgtgta gtgcgggtgg
     1741 tccagcagcc cgcccacgaa ggactcctgc gcggccgacg cttcgtcgcc cagcacgccc
     1801 ggcaactgcc ggatcatggc gtccatcagc accatcgccg gcagctcgcc cccggacagc
     1861 acgtaatcgc cgatcgacac ctcgcaatcc acctgccgca agatcaggcg ttcatccacg
     1921 ccttcgtagc ggccgcacag caggatcagg ccgtcgccct gcagcagctc gcccacgcgc
     1981 gcgtgcgtca gcggactgcc ctgcggcgtg aagtagatca ccctggattc cttcactccg
     2041 cagctgcgct ggcgctggcg cgcggccttg agcgccttct ccagcggttc ggacagcatc
     2101 accatccccg gaccgccgcc gtagggccga tcgtccaccg agcggtaggc gttgctgctg
     2161 aagtcgcgcg ggttccacag caccagctcg tagatcccgt tctcgaacgc ccgccgcgtc
     2221 acgcccgaac gcgtgatggc gtcgaacatc tccgggaaca gggtgacggc gtcgaagtga
     2281 atcaccagtc cgccccccag tccaccgcga cctccccccg cgccagatcc accgactgca
     2341 ccacggcacc cacgaagggg atcagccttt cgcgctcgcc ccggaccacc agcacccggt
     2401 tggcgccggt gtcgatcagc ccggcgatgc gccccagatc ctggtcctgc aggttgcgca
     2461 cccgcagacc ttcgaggtcc gcccagtagt actcgccctc ggcgttgcgc ggcagcgccg
     2521 cgcgcggcac cgccacttcg cagccccgca gtgcggcggc cgcctcgcgg tcgtcgcacc
     2581 ccttcagctt ggccaccacc acgcgcccgt ggacagccgc ttcttcgacc atggcctgcc
     2641 gccactgtcc gtcgcgccgc agccaccagt cggggtagcc cagcaacccg tctggccgct
     2701 cggtgaagac ctggatcctg acccagccgc tgacggcaaa cggggcggcc acccgcccca
     2761 ttatgatcat cgcctccggc gggagtgtac ccatcgccgc cgggggcgcc ttggccccgc
     2821 cgggaccgcc ctcaggccga cgcggcctgc ggctgcgcgg ccttgccaaa ctgcctgacg
     2881 agtctcgcca ccgtgtcgct gagcttggcc cctttgcctt gccagtgtgc cagccgctgc
     2941 agatcgaggc gcagcgactc gcggcccgcg ggcgccttcg gatcgtagaa gccgacgcgt
     3001 tcgatgaaac ggccgtcgcg cggattgcgc gagtcggcca ccaccacgtt gaagaacggg
     3061 cgtttcttgg cgccgccgcg cgcgagtctg atcaccacca tggccagggc ctgccccctc
     3121 ttagcgaagc ccgcgattct acggcttttt tccgcggctt ggcaaggcga tcaacggctc
     3181 gaggcggcgg ccgggtcgcc gccgtggaac acccggtcca tgaaactcgt caggatctgc
     3241 accagctcgt cctcgcgccc ttcgaagaaa tgcagcgcgt ccttgaccac gatctgctcg
     3301 gagcccggga ccttgtcgat ctgccgcttg cgctcgtagg caccgacctg ggtgatctcc
     3361 cagtccttgc tgccgaacac gtccagcacc gggatcttga tgcggaacat ctcctccagc
     3421 ccgttgatga tgccgacgaa cgcccaggcc ttcaccggcg tctgatcgac gttgatcagg
     3481 tactggttcg ccatcgctgc gcccaggctg tgggagacga tgccgatgtt cttgtagccc
     3541 ctggccagca gatacttggc ggcgaggtcg aagcgctccc gagcgtcggg ataggtggcg
     3601 aggtagtcgc cgatctttgc gcccccgccc agcaccggca gctggatggc gagcgtggtg
     3661 taaccccgct cggcgagctt ggtgcgcagc atgccgtaca actcgtagtc cgggctccag
     3721 ccgcggccgt gcgccacgat cagtgcgccg cgcggtttct ccgcctcggc atacagggcc
     3781 aggaatttgt ggccgccggc ctgctggatc cacaccgcct cgccgaccat cagcgacggc
     3841 acgatctggt cggcccagcg tttctcgcga tcgtagtccg aaatgcccca tgcggaaggc
     3901 atcgggccgg acagggccag ggcgatcaac agcatccggt gcagcgtttt catggcgact
     3961 cccgtttcca cacggccgga agacttcggg cgcgttctac cggcgttgga ccggcgtcgg
     4021 ccggcggcgg ttcaacgcat ccccggcagc atgccgcgca agttgcgcat catcttctgc
     4081 atgccgccct tgctcatcat cttcatcatc ttctgcatct gctcgaactg gttgagcagg
     4141 cggttgacct cctgcaccgg gacgccggcg ccgccggcga tgcggcgctt gcgcgaggcc
     4201 ttgatcagct cggggcgggc acgctcggcc ggggtcatgg aatcgatgat gccgatggtg
     4261 cgcgccacca ccttctcgtc cgtgttggcc ccctgggcca tctgcgtgag ctgcgccggc
     4321 agcttgtcca tcaatgcggt gaggcccccc atcttgcgca tctgcagcat ctgctgcttg
     4381 aagtcctcca ggtcgaagcc cttgccgctc ttgaccttct tcgcgagctt ctcggcctgc
     4441 tcgaggtcga ccgtgcggcg cgcctcgtcc accagggaca gaacgtcgcc catgcccagg
     4501 atgcgcgagg ccatgcgttc cgggtggaac ggctcgagcg cgttgagttt ctcgccgacg
     4561 cccaccagct tgaccggctt gccggtgacg tgccgcaccg acagcgtcgc gccgccgcga
     4621 gcgtccccat cgagcttggt gagcacgatg ccggtcaacg gcagcgccgc gccgaacgcg
     4681 ttggcgacgt tgaccgcgtc ctggccctgc atcgcgtcga ccacgaacag cgtctcggcc
     4741 ggctcgagcg ccgcgtgcaa cgccctgatc tcgtccatca tggcctgatc gacggccagg
     4801 cgtccggcgg tgtcgacgat catgacgtcg tggtagtgct tgcgcgcgta gtcgtgcgcc
     4861 gcgcgcgcga tgtcgaccgg gtcgctcgcc gcggataccg gatagaattc cacatcgacc
     4921 tgccgcgcca gcgtctgcaa ctgctcgatg gccgcaggcc gatacacgtc gcagctcacc
     4981 agcaggacct tcttgcgcag gtcctcgtgc agccacttgg cgagcttgcc ggcgctggtg
     5041 gtcttgcccg agccctgcag acccgccatc aggatcaccg cgggcggaac caccgagagg
     5101 tgcagcggcg cgttggccgc tcccatcagc gcggtgagct cgtcgtgcac cacgcccacc
     5161 agcgcctggc cgggtgtgag gctgccggcc acttcgcggc cgagcgcgcg ctccttgacg
     5221 tgcgcgatga agtccttgac cacgggcagc gcgacgtccg cctcgagcag cgccacgcgc
     5281 acctcgcgca gcgcgtcctg gatgttggac tcggtgagcc gcgcctggcc gcgcagcgac
     5341 ttgatcacgc cggacagccg gccagtcaac gattcgagca tggcgtgtgg cgcgcccccg
     5401 gacggggcac aagggatgtt gtagactgcg ggcgcttacg acgcgcggcg cactcggccg
     5461 gcctgcacgc gggcgcgtcg cgagcgtctg agaaccatgc cgggcatttt accccatctc
     5521 gtgtgtgcgg ccctgtacgc cgcggccgcc gtgctgctgt atcgcagcct cgcagggaga
     5581 gacaggccgg gatgggagct cggcgctctg cactgggcga cgctggcgcc gctgctgctg
     5641 cacacctggc tgctctaccg catggtgttc catcccgacg ggatctacct gggggtgggc
     5701 acctcggtct ccgtgatcat ctggctgacg gtcgcgatct actggctggg cagcttcgtc
     5761 taccgcctgc acggcctgca ggtgctcatc ctcggcgcgg ccgcggggct ggtgctgctg
     5821 ccggccctgc tgccgtccgt gcgtccgctg tcgcacgcca acctcgcggc gttccgcctg
     5881 cacctgctga tctcgctgct ggcctacagc ctgttcacca tcgccagcct gcaggcgctc
     5941 atgatggcgg tgctcgagcg ccgtctgcac ggcggcgccc tgccggcgta cctgcaggcg
     6001 ctgccgccgc tgctcacgct ggagcgcctt ttgttccgca tcatcaccgc cggcttcctg
     6061 ctgctcacgc tgacgctggc gagcggaatg ctgttctccg aagcgctgct cggacacccg
     6121 ctgccgttca cgcacaaggt gatcttcggc atcctcgcct ggttgatcta cgcggcgctg
     6181 ctcgccggac gccggctgta cggctggcgc gggcgcacgg cgctgcgctg gacgctgggc
     6241 ggtttcctgg cgctggtgct ggcctacatc ggcagcaagt tcgtgctcga ggtgatcctg
     6301 caaaggtagg ggctgggatt tgggagctag gatctaggaa ggaaggcgcg gtgcaggccg
     6361 cacaaagccc cctagtcccc agctcctagc tcctagcccc tcaggagtca tccaccgtgg
     6421 atgagatccc gctgcacacc ctgctcgccg ccttcgccat cctgctgctc atctcggctt
     6481 ttttctccat cagcgagacc tcgatgatgg cgctcaaccg ctatcggttg aagcacctcg
     6541 cccgcaccgg ccatcgcggc gcgcgcctgg tcgcgcagct gctggagcgc accgacaagc
     6601 tgctgggcgt gatcctgctc ggcaacaacc tggtgaacac cgccgccgcg gcgctgggga
     6661 cggtgatcgc gttccgcgcg atgggtgaga gcgagctggc ggtgtccatc gccaccggcg
     6721 cggtggcctt cttcctgctt gtgttcagcg aggtgacgcc gaaggtggtg ggcgcgacct
     6781 acccggagcg ggtggccttt cccgcggcct tcgtgctcac gccgctgctg aagctcgcct
     6841 accccctggt gtggctggtg aacgggctcg cgggcggcct gctgtggttg ctgcgcatcc
     6901 gccgccagcc ggccgcgcag ggcgcggtgc cgctgagcag cgaagagctg cgcacgctgg
     6961 tgctggaagg gggccactat ctgcccacca agcatcggaa aatcctgctc aatcttttcg
     7021 aactccagtc gataacagtc gctgacctga tgaccccgcg cagccgcctc gaaaccctcg
     7081 atctggacca gccgcccgag gcgttgcggc ggcagatcgc cagcagcaac cacacgcgcc
     7141 tgccggtgta ccgggagacg ctggacaaca tcgcgggcgt tctgcacgtg cgcaaggtgc
     7201 tcaacctgga cgcgagacag gtcgacgcgc aggccctgtc cgaggtgatg cgcccggcct
     7261 acttcgtgcc aatgggcacc acgctgctcg cgcagctgca gaacttccag gaggaggagg
     7321 agcgcttcgg cctggtggtg gacgagtacg gcgagctgat gggcctgatc acgctggagg
     7381 acatcctcga ggaaatcatc ggcgaattca ccacgccctc gccgctcacc gccggcgccg
     7441 tgttgcgtca agacgacggc agctacatcg tcgagggccg catcccgctg cgcgagctca
     7501 accgcgagct ggagctgcag ctgccgctgg aagggccgaa aacgctcaac ggtctgatcc
     7561 tcgagcgcct ggaggacatc ccggagccgg gcaccgtggt caagatcgcc gggtacccga
     7621 tcgagatcct gcagacccag catcgggcga tacgctcggc ccgcatcttc cccaccgagc
     7681 gcgccgactg atcacacttc gtttacaaat cccggcccgc cgcggcatca tttgaaccgc
     7741 ctacaccagg gcatcatttg acccgcctac cccggggcat cattccaaac cgcctaaccc
     7801 cgcttgcaca tggtcgcagc ctccaccgag aaccagctct ccggcctcgg ccgcgcgatg
     7861 gtccagaagg gcctgctcgc cgagcgcgaa gccaccgcct tgcagcacaa ggccaacgag
     7921 gccggcgtga ccttcgtcga gcagctggtc ggcagcaagc gtttcggcga ggtcgaggtc
     7981 gccgagttcg ccgcgcagac cttcggcttc ccgctgctcg atctgcaggc cttcgacctg
     8041 gaccacttgc cgtccaagca catcgacccc aaggtgctcg gcggccgccg cgcgctgccg
     8101 ttgttccagc gcggcaatcg cctgttcgtg gcgatctccg atcccaccaa ccgggcggcg
     8161 atggaccaga tcaagttcca gaccgggctg cacatcgagc cggtggtggt ggaggacacc
     8221 aagctcggca tcctgatcgg caagctggtc gccgccagcg gcgccgcgct gagcgagctg
     8281 gtggccgagg acatggccgc cgagctgacc gaggccgacc ccgagccgca ggccgacagc
     8341 cagtcgatcg agatcgacga cgcgccggtg gtgaagtaca tccagaagat catgctggac
     8401 gcgatcgaca gcggcgcttc cgacatccac ttcgaaccgt acgagaaggt ctaccgcatc
     8461 cgctaccgcc tggacgggga gctgtacgag gtcgccaacc cgccgctggc catcaaggag
     8521 aagatcgcct cgcgcatcaa ggtgatctcg aggctggaca tctcggagaa gcgcgtgccc
     8581 caggacggac gcatgaagct ggtgctgtcc aagacccgcg ccatcgactt ccgcgtgagc
     8641 acgctgccga cgctgttcgg cgagaagctg gtgctgcgca tcctcgaccc ttcgagcgcc
     8701 agcctcggca tcgacgcgct gggctacgac ccggatcagc gcgaggcact gctcaacgcc
     8761 gtgcaccggc cttacggcat ggtgctggtc accggtccca ccggctcggg caagaccgtg
     8821 tccctgtaca cctgcctgaa catcctgaac cagcccgaca tcaacatcgc cacggccgag
     8881 gatccggcgg aaatcaatct ccccgggatc aaccaggtga acgtcaacga gcgcgctggc
     8941 ctgacttttg ctgttgcgct cagggctttc ctgcgccagg acccggacgt gatcatggtc
     9001 ggcgagatcc gcgatctgga gaccggcgag atcgccgtga aggcggcgca gaccggtcac
     9061 ctggtgttgt cgacggtgca caccaacgac gcgccgagca ccttgacgcg cctcatgaac
     9121 atgggcatcg ctccgttcaa catcgcctcg tcggtgatcc tgatcacggc gcagcgcctg
     9181 gcgcggcgcc tgtgctcgca ctgcaagcgt cccgcggacg tgcccaagga agcgctgctg
     9241 cgcgccggct tcagtgagcg cgacctggac gggcgctgga cgccctacgc gcacgtgggc
     9301 tgcgaacact gcaagaacac cggctacaag ggccgcgtgg gtgtgtacca ggtgatgccg
     9361 atgagcgaag cgatcaacca catcatcctg acgagcggca acgccatgga actcgccgca
     9421 caggcgcgca gggagggcgt gcgcgacctg cgccagtccg gcctgctgaa ggtgaggcag
     9481 ggcgtcacgt cgctcgagga agtggaagcg gtgaccaacg agtagggcac ggggagagtc
     9541 acgacgatgg caactgcaca agcgacgtcg gcggcgcgtc gggatgtcaa ggaattcatg
     9601 ttcgcctggg agggccgcga ccgcggcggc aagctggtca agggcgacat gcgggcgagc
     9661 ggcgaggcgg tggtgcaatc cacgctgcgc cggcagggca tccacgtgct caagatcaga
     9721 cgccagcgcg gcggcgtcgg cggcaagatc accgagaagg acatcacgct gttcacccgc
     9781 cagctcgcca ccatgatgaa atccggcgtg ccgctgctgc agtcgttcga tatcgtcggc
     9841 aagggccact ccaacccggc ggtgggaaag ctcctgctcg acatcaagac cgacgtggag
     9901 accggcagct cgctgaacca ggcgttccgc aagtacccgc tgcatttcga cgcgctgttc
     9961 tgcaacctgg tggccgccgg cgaggccgcc ggtatcctgg acaacctgct cgaccggctg
    10021 gcgagctaca aggagaagat cctcgccatc aagagcaaga tcaagtcggc gctgttctac
    10081 ccgatcgcga tcatcgtagt cgccttcctg atcacggcgg tgatcatgat cttcgtgatc
    10141 ccggcgttca aggagctgtt caccaacttc ggcgccgacc tgcccgcgcc cacggtgatg
    10201 gtgatgaacc tttcggatat cttcgtcacg tactggtatc tgatcttcgg caccatcggc
    10261 ggcgggctgt ggttcttctt ctacacctgg aagcgctcgg ccaagatgca ggcggcgatg
    10321 gaccgcgctt tcctgaagct gccggtgttc ggcgacatca tccgcaaggc caccatggcg
    10381 cgctggagcc ggacgcttgc caccatgttc gccgccggcg tgcccctggt ggaagcgctg
    10441 gactcggtgg gtggcgcctc gggcaactac gtgtacctga tcgccaccaa gaagatccag
    10501 caggaagtga gcaccggcac cagcctgacg gtgtcgatgc agaacagcga agtgttcccc
    10561 aacatggtga tccagatggt ggcgatcggc gaggagtcgg gctcgctcga cgccatgttg
    10621 agcaaggtgg ccgatttcta cgagcaggaa gtggacgacg ccgtggaagc gctgtccagc
    10681 ctgatggaac cgatgatcat ggtggtgctg ggcacgctga tcggcggcct ggtgatcgcc
    10741 atgtacctgc cgatcttcaa gatgggccag gcgatctagg accggcacgc gcgcggccgg
    10801 caggcggcgc gcgccccgca tcgcgcgtga ggtcgtgggc atcgcccgcg ccggcgcgca
    10861 gcaccgctgc ccccggctac cgcccgcatg tcgacgcttc agctgctcgc ccactcccca
    10921 gacctgttcg gcggcgtgtg cctgttgctc gggctgctgg tgggcagctt cttgaacgtg
    10981 gtgatccacc ggttgccggt gatgctcagg cgcgactggc gccggcagtc ggtggaaatc
    11041 ctgagcgagt ggtcggagga gaaagagatc cccggcgagc tggcgcagat gcgcgccggg
    11101 ctggagaagc tgggtccggc gctcgccgcg gcgcgggcct acaacctgat cgtgccgcgc
    11161 tcggcctgcc cgcgctgcgg ccatcggatc ggtgcgctgc agaacatccc ggtggtgagc
    11221 tatctggtgc tgcgcggacg ctgcgccggc tgcaacgccc gcatcagccc gcgctacccc
    11281 gtgatcgaag ccttcaccgg gctggtctcc gcctgcgtcg gctggcactt cggcttctcg
    11341 ctggccgcac tggccggcct gctgttcgcc tgggcgctga tcgccgccgc ggtgatcgac
    11401 ttcgacaccc agctgctgcc cgacgatatc accctgccgc tcacctggct cggtctgtcg
    11461 ctcaacctgt ggggcgcgct caccacgctg gagtcggcgg tgatcggcgc cgtggccggg
    11521 tacctggcat tgtggtctgt ctactgggtg ttcaagctga cgaccggcaa ggaggggatg
    11581 ggtttcggag acttcaagct gctcgcggcg atcggcgcct tcctcggctg gaagatgctg
    11641 ccggcggtga ttctgctgtc ctctctggtc ggcgccgtgg tgggcatcgg gctgatcgcg
    11701 ctggccaagc gcgggcgcaa cgtcccgata ccgttcggtc cctatctcgc cgccgcgggg
    11761 ctgctcgcgc tgttcttcgg cgaggacctc aaccgcgcct atctgcagct gctgtgatgc
    11821 tggtcgtcgg cttgacaggc ggcatcggtt ccgggaagag cgcggtagcg gcgctgttcc
    11881 aggcgctggg tgccgcggtg atcgacaccg acgtcatcgc gcacgagctc accgcgccgg
    11941 ggcagccggc cgtcgagcag atccgggccc gcttcggtgc gcactacgtc accgcgcagg
    12001 gtgcgctcga ccgcgctcgc atgcgcgagc tggtgttcgc cgacgccgcg gccaagcgcg
    12061 acctagaggc gatcctgcac ccgcgcattc gcgaacaggc ggccgcacgg gtggcgcaag
    12121 ccggcacgcc ctacgccatc ctggtggttc ccctgctggc cgagaccggc ggctggcccg
    12181 ggctgatcga ccgggtgctg gtggtggatt gcgaagagag cttgcagaca gagcgcaccc
    12241 ggcgccgcag cgggctcagc gccgaccagg tgcaggcgat catggcccgg caggccaccc
    12301 gcgcgcagcg cctggcgctc gccgatgacg tgatcgacaa cagcggaccg gtcgaagcgc
    12361 tggaagagca ggtgcggcgt ctgcacgcgc gctatctgtc gctggcgcag gcatgagtcc
    12421 ggccgcccgc gtcatgggcc tgctttctcc cgaagattca ttcttgccgg gtttattttt
    12481 ccgggttttt gggcaatact cccggcagcc atcgaagccg tctcgggtcg acgccaggtg
    12541 atcagctatg aattcccgct caacgagcgc attcgcaccc tgctgcggat ggagagcctg
    12601 tatgagaggc tgcgcttctt cgtcgagcac gacgagcgcc tggagcacca cgccgccctg
    12661 ctcacgctgt tcgagtttct ggaggtcgcc ggccgcgcgg acttgaagtc cgaggtgatc
    12721 caggaactgg agcgccagcg ccagagcctg agcgcattgc gctccaaccc ggccatctcc
    12781 gagcgcgcgc tgagcgaagt cctcgatgac atcgaccggg ccgtgcaggg catgcagcag
    12841 tcctccggca agaccgggca ggagctgcgc gagaacgagt ggctgatggg catcaagcag
    12901 cgcgccgcca tcccgggcgg cgtgtgcgag ttcgatctgc cgtcctatca ccactggctg
    12961 caccagcccg ccgccgatcg ccggcgcgac ctcgagagct ggatcacgcc gttcctgccg
    13021 ctgcagcagg gcgtgggcat catcctgcgc atcctgcgcg acagcggacg caccggccag
    13081 cagctggccg cacagggcgt gtatcagcag atgatgggcg gacgcctggc acagctgctg
    13141 cggctgcgcg tggacgacgc ctacgactgc gtgcccgagg tgagcgccaa caagtacgcg
    13201 ctcaacatcc gcttcaccgt gggcagcggg cagcagcgcc cgcgcgtgag ccagaccgac
    13261 atccccttcg agctgacgtt ctgcaacctc tgatggccga ggaccgcagg aaagtggtga
    13321 tctgcccgca gtgcggggcg cccacccctt ggagcgagct caatccccac cgtccgttct
    13381 gctccgagcg ctgcaagctg atcgacctcg gccagtgggc ctcggaggga tatcgcatcc
    13441 cagtcgcgca ggatgagtcg gtgtccggcg atgaaaccgt gaccgagaaa cgtgaccgaa
    13501 cggcgtgacc gaaaagcgtg acgaacaccg cgtagcccgg atgcagcgta gcggaatccg
    13561 gggaagtaca aggctttgct ctcccggatt ccgccgcttg cgcggctgca tccgggctac
    13621 ggtgtcacgc ggtcacgctt gtcggtcacg tcgttcggtc acgtcgttcg gtcacgtctg
    13681 tcggtcacgt ctgtcggtca cgctgtttcc cacgccgccc gcatcatcgc gatgccgtgc
    13741 gcgcccgcca gccacgcgcg ctccagatgc cgcggctgca tgccgcccag ggcgtacacg
    13801 ggcagcgtgc agtcggcgat cagttccgcg aagcgctccc atcccagcgc cggctgcccg
    13861 gggtggctcg ccgtgggcaa cacggcgccc agcaccgcgt agtccaggtc cagccgctgc
    13921 gcctgctgga gctcaccggc atcgtggcag gacgcgccgc acagacccgc cgagggccgg
    13981 gtgctcgcct gcagcagggc cggcgcagac aggtgtacgc cgtcggcgcc acacgccgcg
    14041 gcaagccggg cgtcgccatg gatgagcgcc tgcgcgcccg ccgcatggca gcgccgcacc
    14101 acctcgcccg cgaacacggc cagcgcgtcc gcccgcatct tcttctcgcg caccatcacc
    14161 atgcgcagtc cgcgcgccag cgcagcgtcg agctgccgca ggaacggctc gagcc
//
LOCUS       DASPGE010000395         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_340020, whole genome shotgun sequence.
ACCESSION   DASPGE010000395 DASPGE010000000
VERSION     DASPGE010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340020"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VD791_12935"
     CDS             <1..1140
                     /locus_tag="VD791_12935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HXV08932.1"
                     /translation="EHPAAAARVSPALRTNLGKRAGVASGLLGALSVLGALCFLLPDF
                     LVTKDAMPFYAAHLGVFRAILWAAIGATFVLAATGLLLLRSKAHGMLGLLLGAVALLM
                     GGGAAEPLAIDGDPAYALGLDYFVLELLVLGLLFIPLERLWSLRSQRIFRPGWQTDLK
                     HFFVSHAGVQLLSFAAMVPVHSLFAWAVQLEFQKAIAAQPLWLQFVEILLVVDFMTYW
                     VHRAFHQVPWLWSFHAIHHSSRQMDWLAGSRTHIVDVLATRAAAYLPVFLLGFAPGAL
                     YAYLAFVSVHAVFIHANIRWRFPYLRWAIATPEYHHWHHTSDEEGLDKNFAAFLPLWD
                     LAFGTAHQPEHWPRNYGTVKFQPPEDYLGQLLYPFRRHGKRTPYG"
     gene            complement(1137..1874)
                     /locus_tag="VD791_12940"
     CDS             complement(1137..1874)
                     /locus_tag="VD791_12940"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HXV08933.1"
                     /translation="MRDVRGWIELAERVVALTGAGISTDSGIPDFRGPQGVWTRNPKA
                     EKLSDIRHYMSDPEVRRLSWQSRLEHTAWRAQPNPGHRALVALEKRGKLHALITQNID
                     ELHQIAGNSPDKVIEVHGTLRKVVCMSCGERAPMEKALARVRAGEVDPPCRSCGGILK
                     SATISFGQALVPEVIDRAMQAAAEAEVLLAVGTSLQVYPIAGAVPAAKAAGARVVIVN
                     AEPTPFDDLADAVFNDSISEVLPRLLG"
     gene            complement(1891..>2129)
                     /locus_tag="VD791_12945"
     CDS             complement(1891..>2129)
                     /locus_tag="VD791_12945"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXV08934.1"
                     /translation="DPIHLGIMLTLAVEMSVVTPPVGLNLFAVSGTSKIPVTQVLKGS
                     VPFFLGDLIVLLLVIFFPILATWLPGQLTTTVFK"
BASE COUNT          290 a          791 c          682 g          366 t
ORIGIN      
        1 gaacaccctg ctgcggcggc gcgcgtgagc cccgccctgc gcacgaacct cggcaagcgc
       61 gccggcgtcg cgtccggctt gctgggagcc ctcagcgtgc tcggcgcgct ctgcttcctg
      121 ctgccggact tcctggtgac gaaggacgcg atgcctttct acgccgccca cctcggcgtc
      181 ttccgggcga tcctgtgggc cgcgatcggc gcaacgttcg ttcttgcagc gaccggtctg
      241 ctgctgctgc gctccaaggc ccacggcatg ctggggctcc tgcttggtgc cgtggcgctc
      301 ttgatggggg gcggtgcagc cgagcccctc gcgatcgacg gcgatccggc ctacgccctc
      361 ggcctggact atttcgtgct ggagctcctg gtcctcggcc tgcttttcat cccgctcgag
      421 cgcctgtgga gcctgcgcag ccagcgcatc ttccgccccg gctggcagac cgacctcaag
      481 cacttcttcg tcagccacgc gggcgtgcag ctgctgtcct tcgctgccat ggtgccggtg
      541 cattcgctct ttgcctgggc ggtgcagctc gagttccaga aggccatcgc ggcgcaaccc
      601 ctgtggctgc agttcgtgga gatcctgctg gtcgtcgact tcatgaccta ctgggtgcac
      661 cgcgccttcc accaggtgcc ctggctgtgg agcttccacg ccatccacca ctccagccgc
      721 cagatggact ggctcgcggg gtcgcgcacg cacatcgtcg acgtgctggc gacgcgcgcg
      781 gcggcctatc ttcccgtgtt cctgctcggc ttcgcccccg gcgcgcttta cgcctacctc
      841 gctttcgtct ccgtgcatgc cgtcttcatc cacgccaaca tccgctggcg cttcccctac
      901 ctgcgctggg cgatcgccac gccggaatac caccactggc atcacacctc cgacgaggag
      961 ggcctggaca agaacttcgc cgccttcctg ccgctctggg acctcgcgtt cggcaccgcg
     1021 caccagcccg aacactggcc gcggaactac ggcacggtga agttccagcc gccggaggac
     1081 tacctggggc agctcctcta ccccttccgg cggcacggga agcgcacgcc ctacggctag
     1141 ccgaggagcc tgggcagcac ctcgctgatc gagtcgttga acacggcgtc ggcgagatcg
     1201 tcgaagggcg tcggctcggc gttgacgatc accacgcgcg ccccggccgc cttggccgcc
     1261 ggcaccgcgc cggcgatcgg gtagacctgc aggctcgtgc cgacggcgag cagcacctcg
     1321 gcctcggccg ccgcctgcat ggcgcggtcg atgacctccg gcaccagcgc ctgcccgaag
     1381 gaaatcgtcg cgctcttcag gatccccccg cagctgcggc agggcggatc gacctccccg
     1441 gcgcgcacgc gcgccagggc cttctccatg ggcgcgcgct cgccgcagct catgcacacg
     1501 accttgcgca gggtgccgtg cacctcgatg accttgtccg gagagttgcc ggcgatctgg
     1561 tgcagctcgt cgatattctg cgtgatgagc gcgtgcagtt tgccgcgctt ctccagcgcc
     1621 accagcgcgc ggtggcccgg attgggctgc gcgcgccacg ccgtatgctc cagccggctc
     1681 tgccaggaca ggcgccgcac ttccgggtcg ctcatgtagt ggcggatgtc cgagagcttc
     1741 tccgccttgg ggttcctggt ccacacgccc tgcgggccgc ggaaatccgg gatgccggaa
     1801 tcggtggaga tgcccgcccc ggtgagcgcc accacgcgct cggccagctc gatccagccg
     1861 cgcacatccc gcatgcgcgc acgcccggcg ctacttgaac acggtcgtcg tcagctggcc
     1921 cggcagccag gtggcgagga tcgggaagaa gatcaccagc agcagcacga tcaggtcgcc
     1981 gaggaagaac ggcaccgagc ccttcagcac ctgcgtcacc ggtatcttgg aggtgccgct
     2041 cacggcgaac aggttcaggc ccaccggcgg cgtcaccacg ctcatctcca ccgcgagcgt
     2101 cagcatgatg cccaggtgga tcgggtcga
//
LOCUS       DASPGE010000396         3713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1627751, whole genome shotgun sequence.
ACCESSION   DASPGE010000396 DASPGE010000000
VERSION     DASPGE010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627751"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="VD791_12950"
     CDS             complement(<1..163)
                     /locus_tag="VD791_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HXV08935.1"
                     /translation="MPDRQYGIGTLCLHAGQIPDPTTGARAVPIYQTTSYVFDSADHA
                     ASLFNLQTFG"
     gene            complement(208..2601)
                     /locus_tag="VD791_12955"
     CDS             complement(208..2601)
                     /locus_tag="VD791_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXV08936.1"
                     /translation="MHGYASNALALSLALTAATVIAVGLAIALRERFSRVGWLHLYLA
                     CAAGGYQAALAAVMLAEDETLALQWMRLGTFAALLIAPLAYQFGCTVTGQRIAQRRGL
                     VVVWAATALLALGHAAGLLFSGLHRYDWGAYGAYTAVGWLFVLLTFGTVALALRVYWS
                     EYRNRSRKLSSQRALRLGCALLLGSFAVVDFLPALGHNVYPLGGLALLGAAAINAHTM
                     WRYRLVEITPAYAADQLMDSMSDGVLMIDRDGVVRLVNPGAAEILGIDRANLVDRLPP
                     ATLATEVLGWQQLPFFPSTDMALGERSYVTPDGTPRVLDVSVVLMRDYGLEPAVAVIG
                     LRDITEKLQAREQIERLAYYDPLTHLPNRLLLRDRFGDAIARAKRANGMAATLFMDLD
                     RFKQINDTLGHDAGDMLLKAVAERIGACVRETDWLLRGSEMSNSSMLARLGGDEFVVL
                     LSPIARPEDAAKVATRILEALARPVSLKRGAEVTSGASIGISIFPHDGDEPETLMKKA
                     DLAMYQAKEAGRNTFRFHDEAMNASMLKRTDLHNSMRRGLHRSEFLLHYQPQVACRSR
                     EIAGLDAQVFWRHPQHGLMPAAEFVSASEDSSVVVPLTEWVVRSACMQLRAWVAQGLP
                     TLYLSLTLPPGAAERGDLPRLVRDSLEQAGVDPGLVMVSLRGGPNPREAQRTRDAMQT
                     LQSMGARLVLDDFGSGDMTLSSLGQYPLGMVRFESAFLRGLARDGDLASVTRSLISVV
                     HALNLGVIVTGVDNPAQAAFLREVACDLAQGAAFGAPVPAEDVPGLLSDIREQLAAG"
     gene            2963..>3713
                     /locus_tag="VD791_12960"
     CDS             2963..>3713
                     /locus_tag="VD791_12960"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV08937.1"
                     /translation="MSAAFTGTAAALGTELYRGAQAYYDEINDRGGIHGRPISVALLD
                     DRYEPLPCVMNTLRLLEEEAVFLLSGYVGTPTLTRALPFIMHYAREHDEDLAVVGVYT
                     GAQPQREPPYVQYVFNIRASYRQEMMGLVDQFWRAGAREFGVFYQIDAYGRSGTDGVA
                     RGLARRGTTIAVEATYARGAKFEDDMSLAVKALRKGGAEVVLCTGAYQGCAAFIRSAR
                     DLGWNVPISNLSFVGSEALLGLLTEHGRKKGR"
BASE COUNT          588 a         1232 c         1322 g          571 t
ORIGIN      
        1 tgccgaaggt ctgcagattg aacaggctgg ccgcgtggtc ggccgagtcg aacacgtagg
       61 acgtggtctg gtagatcggc acagcgcgcg cgccggtggt cggatcgggg atctgtccgg
      121 cgtgcaggca cagcgtgccg ataccgtatt gacggtcggg catttgggat ctcggggagc
      181 tgcgaggagg gagattctac agcgccgtca acccgcggca agctgctcgc ggatgtcgga
      241 cagcagcccc ggcacgtcct cggcgggaac cggcgcgccg aaggcggcgc cctgcgccag
      301 gtcgcaggcg acctcgcgca ggaaagcggc ctgcgcgggg ttgtccaccc cggtgacgat
      361 gaccccgagg ttgagcgcgt gcaccaccga gatcagcgag cgcgtgacgc tcgccaggtc
      421 gccgtcgcgc gcgagcccgc gcaggaacgc ggattcgaag cgcaccatgc ccagcggata
      481 ctggcccagg ctggagagcg tcatgtcgcc gctgccgaag tcgtccagca ccaggcgcgc
      541 gcccatcgac tgcagtgtct gcatcgcgtc gcgcgtgcgc tgcgcttcgc gcggattcgg
      601 cccgccgcgc aggctgacca tcaccaggcc cgggtccacg cccgcctgct ccagcgagtc
      661 gcgcaccagg cgcggcaggt cgccgcgctc ggccgcgccc ggaggaaggg tgagcgagag
      721 gtacagagtg ggcaatccct gggccaccca ggcgcgcagc tgcatgcagg cgctgcgcac
      781 cacccactcg gtgagcggca ccaccaccga gctgtcctcg ctggcgctga cgaactcggc
      841 cgcgggcatc agcccgtgct gcggatggcg ccagaacacc tgcgcgtcca ggccggcgat
      901 ctcgcggctg cggcaggcca cctgcggctg gtaatgcagc aggaactcgc tgcgatgcag
      961 gccgcggcgc atgctgttgt gcaggtcggt gcgcttgagc atcgaggcgt tcatcgcttc
     1021 gtcgtggaag cggaaggtgt tgcggccggc ctccttggcc tggtacatgg ccaggtcggc
     1081 cttcttcatc agcgtctcgg gctcgtcgcc gtcgtgcggg aagatgctga tgccgatgct
     1141 ggcgccgctg gtgacctcgg cgccgcgctt caggctcacc ggtcgcgcca gcgcttccag
     1201 gatgcgtgtg gcgaccttgg ccgcgtcctc gggacgggcg atgggcgaga gcaggaccac
     1261 gaactcgtcg ccgccgaggc gcgcgagcat gctgctgttg ctcatctccg agccgcgcag
     1321 cagccagtcg gtctcgcgca cgcaggcgcc gatgcgctcg gcgaccgcct tgagcagcat
     1381 gtccccggcg tcgtggccca gggtgtcgtt gatctgcttg aagcggtcga ggtccataaa
     1441 cagcgtggcg gccatgccgt tcgcgcgctt ggcccgggcg atggcatcgc cgaagcgatc
     1501 gcgcagcagc agccggttgg gcaggtgcgt gagcggatcg tagtacgcga ggcgctcgat
     1561 ctgctcccgc gcctgtaatt tttcggtgat gtcgcgcagc ccgatcaccg ccaccgccgg
     1621 ctccagcccg tagtcgcgca tcagcaccac gctcacgtcc agcacgcgcg gggtgccgtc
     1681 tggcgtcacg tagctacgct cgccgagcgc catgtcggtc gaggggaaga acggcagctg
     1741 ctgccagccg aggacctcgg tcgccagggt ggccggcggc aggcggtcga ccaggttggc
     1801 gcggtcgata ccgaggatct cggcggcgcc cggattgacc aggcgcacca cgccgtcgcg
     1861 gtcgatcatc agcacgccgt cgctcatcga gtccatcagc tggtcggcgg cgtaggccgg
     1921 cgtgatctcc accagccggt agcgccacat ggtgtgcgcg ttgatcgccg ccgcacccag
     1981 cagggcgagg ccgccgaggg ggtagacgtt gtggcccagc gccggcagaa agtccaccac
     2041 ggcgaacgag ccgagcagca atgcgcaccc gaggcgtagc gcccgctgcg aggacagttt
     2101 tctgctccgg ttgcggtact cgctccagta gacgcgcagc gccagcgcca ccgtgccgaa
     2161 ggtgagcagg acgaacaacc agccgaccgc ggtataggcc ccgtacgctc cccagtcgta
     2221 gcggtgcaag cccgagaaca gcagaccggc ggcatggccc agggcgagca gcgcggtggc
     2281 ggcccatacg acgaccagcc cgcgccgctg cgctatgcgc tgtccggtga cggtgcaacc
     2341 gaattgatag gcgagggggg cgatcagcag cgcggcgaag gtgccgagcc gcatccattg
     2401 cagcgccagc gtctcgtcct cggcgagcat caccgcggcc agcgccgcct ggtagccgcc
     2461 ggccgcgcag gccagataca ggtgcagcca gccgacgcgc gagaagcgct cgcgcagggc
     2521 gatcgccaag ccgacggcga tgacggtggc cgcggtcagc gcaagagaca gcgccaacgc
     2581 gttcgacgcg tagccgtgca ttgtgaacgg tttcaggttc cccgtttacc gccgacgagg
     2641 cgccggcggg caggtttggt tttacggggt tatcggcagg ctgatcccgg gcttaaggga
     2701 ggacgccctg gttccatcgc atttgcgctg gcggaggact cagtttagtc tgtgctacgg
     2761 ttcatcgccc tgagcccagg actcttggca tatggaggca aagcgcaagc tccctcggcg
     2821 acggtttctt cgcctggtgg ggggcggcat cacgagcgca gtcttggcgg gcgcgtcggg
     2881 cgccgcccgg gcgcggcgca cattctcggc gcagaccggc gctcccaggc gcgccgtaac
     2941 tgctcgggcc atcaagatcg ggatgagcgc cgccttcacc gggacggccg ctgcgctggg
     3001 cacggaactg taccgcggcg cccaggctta ctacgacgag atcaacgacc gcggcggcat
     3061 ccacggccgg cccatctcgg tagcacttct cgacgatcgc tatgagccgc tcccgtgcgt
     3121 catgaacact ttgcgcttgc tggaagaaga agcggtgttc ctcctgtcgg gttacgtcgg
     3181 aacgcccacg ctgacgcggg ccttgccgtt catcatgcac tacgcgcggg agcacgatga
     3241 agacttggcc gtggtcggcg tctataccgg cgcgcagcct cagcgggagc ctccgtatgt
     3301 ccaatacgtg ttcaacatcc gggcctccta ccgacaggag atgatggggc tggtagacca
     3361 gttttggcgc gccggtgcgc gtgagttcgg cgtcttctac cagatcgacg cctatggccg
     3421 gagcggaacc gacggagtag cgcgcggcct cgctcgacgg ggcaccacca ttgccgtcga
     3481 ggcgacctat gcgcggggcg cgaaattcga ggacgacatg agccttgcgg tcaaggcgct
     3541 gcgcaagggc ggtgcagagg tggtgttgtg caccggcgca taccaaggct gcgcggcatt
     3601 cattcgatcg gccagggacc tcggctggaa cgtgccgatc tcgaatctct ccttcgtcgg
     3661 ctcggaggcc ctgctaggcc tgctcaccga acatggccgc aagaaggggc ggg
//
LOCUS       DASPGE010000397         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_222032, whole genome shotgun sequence.
ACCESSION   DASPGE010000397 DASPGE010000000
VERSION     DASPGE010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222032"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VD791_12965"
     CDS             complement(<1..790)
                     /locus_tag="VD791_12965"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HXV08938.1"
                     /translation="MGARGHKFVLLLSVTLTGMAVLVVEISAVRMLAPFFGNSIFTFS
                     SVIGIVLAALSLGYYVGGRLADRRPSEAWFFSLIVVSGFSVLLLQVLNAVLLPAIAYK
                     LSMVNGPLLVSLLMFFVPALFLAMLSPYAITLLHARATGGGVGNAAGLVFFWSTLGSI
                     AGSLGTGFLLIPHWGIGSIMIGVGSGLVLLGGAGLLMTRKTPRLFPVGLMLLGLISAM
                     ALRQIGAEERADVVYAAEGLYERIVVRDMRHQGRPVRILWQDRNM"
     gene            838..1566
                     /locus_tag="VD791_12970"
     CDS             838..1566
                     /locus_tag="VD791_12970"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HXV08939.1"
                     /translation="MKLPFAGLRLVALCLTLLPALAAAATPSIGRVSQITGDRLGNAQ
                     APGAPARTLAAGDAVFAGERIRTGARTVLQIEFTDKTRFTLGPNAEFEVSRYSHAAGG
                     AAEEESFHSRIFKGAFRFVSGLVARTKRKDMRVSVSVATIGIRGTHVEGEVIERREKD
                     GAMVDASAKVALLEPEGADAPSAIVVENQFGSVVIDKPGYGTEIPDEKSPPSPVKRMQ
                     LRTIDNVTRAIRSPARTGGPARRP"
     gene            complement(1579..>2112)
                     /locus_tag="VD791_12975"
     CDS             complement(1579..>2112)
                     /locus_tag="VD791_12975"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HXV08940.1"
                     /translation="LPVFRQRAGRRRVVYFPGSTIGNLAVAEAQAFLSMSRAQVGADG
                     AMLIGVDVKKDANVLHAAYNDARGVTARFNLNLLARVNRELGADFDLRRFRHYAFYNA
                     PLGRVEMHLVSLARQAVRIGRHRFEFERGETIHTENSHKYAVEEFRALAAEAGFRGAK
                     LWQDRQGQFSLHGLVAD"
BASE COUNT          380 a          701 c          748 g          283 t
ORIGIN      
        1 tcatgttgcg gtcctgccag aggatgcgca ccgggcggcc ctggtgccgc atgtcgcgca
       61 cgacgatcct ttcatacagg ccttccgcgg cgtaaacgac gtccgcccgc tcctcggcgc
      121 cgatctgcct gagcgccatg gccgaaatga ggccgaggag catcaggccc accggaaaca
      181 gtctcggcgt cttgcgcgtc atgagcagtc cggcgccgcc cagcagcacc aggcccgatc
      241 ccacgccgat catgatgctg ccgatgcccc agtgcgggat cagcaggaag cccgtgccca
      301 ggctgcccgc aatgctaccg agcgtggacc agaagaacac cagcccggcc gcgttgccca
      361 cgccgccgcc cgtggcgcgc gcgtgcaaga gcgtgatggc gtacggcgac agcatcgcca
      421 ggaacagggc gggcacgaag aacatcagca gggaaacgag cagcggtccg ttgaccatgg
      481 acagcttgta ggcgatggcg ggcagcagga cggcgttgag gacctgcagc aacaggaccg
      541 agaagcccga gaccacgatc agcgagaaga accacgcctc ggacggcctg cggtcggcga
      601 gcctgccgcc cacgtagtag ccgaggctca gcgccgccag cacaatgccg atgacgctcg
      661 agaaggtgaa gatggagttg ccgaagaagg gcgccagcat gcgcacggcg gagatctcta
      721 cgacgaggac cgccatgccg gtgagcgtca cggacaacag gagtacgaac ttgtgcccgc
      781 gagcgcccat tgctggataa tcaggctcac gcgaggaaat cgtcaaggag cgggccaatg
      841 aaactcccct ttgctggatt gaggctggtt gctctctgcc tgacgctgct gcccgcgctc
      901 gccgcggccg cgacgccgtc catcggacgg gtatcgcaga tcacgggcga ccggctcggc
      961 aacgcgcagg cgccgggggc gcccgcgcgg acgctggcgg cgggcgatgc cgtcttcgcc
     1021 ggagagcgca ttcgcacggg cgcgcgcacc gtcctgcaga tcgaattcac ggacaagacg
     1081 cgcttcacgc tgggaccgaa cgcggagttc gaggtttcgc gctacagcca tgccgcgggc
     1141 ggcgccgccg aggaggagtc ctttcactcc aggatcttca agggggcctt ccgcttcgtt
     1201 tccggcctgg tggcgcgcac caagagaaag gacatgcgcg tcagcgtgag cgtcgccacg
     1261 atcggcattc gcggcacgca tgtcgaaggc gaagtgatcg agcggcggga gaaggacggg
     1321 gcgatggtgg acgcctccgc caaggtggcg ctgctggagc cggagggcgc ggatgcgccc
     1381 agcgccatcg tcgtggagaa ccagttcggc agcgtggtca tcgacaagcc gggctacggc
     1441 accgagattc cggacgagaa gagcccgccc tcgccggtga agaggatgca gctgcgcacc
     1501 atcgacaacg tgacgcgcgc gattcgttcg ccggcgcgga ccggcggccc ggcccgcagg
     1561 ccctagcggc gcagcacgct agtccgccac caggccgtgc agggagaact ggccctggcg
     1621 gtcctgccag agcttggcgc cgcggaatcc cgcctcggcg gcgagcgcgc ggaactcctc
     1681 gaccgcgtac ttgtgggaat tctccgtgtg gatggtctcg ccgcgctcga actcgaagcg
     1741 gtgccggccg atgcgcactg cctggcgcgc gagcgacacg aggtgcatct ccacgcgccc
     1801 gagcggcgcg ttgtagaacg catagtggcg gaagcgccgc aggtcgaagt cggcgccaag
     1861 ctcgcgattg acgcgggcga gcaggttcag gttgaagcgt gcggtgacgc cgcgcgcgtc
     1921 gttgtacgcg gcgtgcagca cgttggcgtc cttcttcacg tccacgccga tcagcatggc
     1981 gccgtccgcg ccgacctggg cgcggctcat cgacaggaac gcctgcgcct ccgccaccgc
     2041 caggttgccg atggtggagc cggggaaata gaccacgcgc cggcgcccgg cgcgctggcg
     2101 gaacacgggc ag
//
LOCUS       DASPGE010000398         3230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_222135, whole genome shotgun sequence.
ACCESSION   DASPGE010000398 DASPGE010000000
VERSION     DASPGE010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222135"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="VD791_12980"
     CDS             <1..1510
                     /locus_tag="VD791_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HXV08941.1"
                     /translation="GGKDDWFWIDIPVGYLYTIGNPRTDWCYKTEPDPGLNGRRIGYA
                     RGKVLGGCSSINAMIYMRGQKSDYDHWAALGNRGWSWDEVLPWFKRTEDYQHGADALH
                     GAGGELRVEERRVNWEILDAWREAAEECGIPKIREFNRGDNFGNAYFQVNQRRGVRWS
                     ATKAFLRPVQHRPNLAVLTGALAHRVRIDTHDGVKRATGVELSLSDGMRTVEARQGVI
                     LAAGAIGSPQILQLSGVGPAALLQHHAIPVVHDLPGVGENLHDHLQIRLMYKVHGVTT
                     LNQRANSLLGKAAMGLEYLLFKTGPLTMPPSQLGAFARSDPSQPSANIEWHVQPLSLD
                     RFGEPLHPFPAITPSVCNLRPASRGHVRITSADPRAHPAITLNYLSAEADRRVAVDSM
                     RFTRRIMAASALAKYSPEEWKPGPSAQSDEALLKAAGDLGTTIFHPVGTCKMGHDEFA
                     VVDDRLRVRGIEGLRVIDASVMPRITSGNTNAPTVMIAEKGAAMIAEDLSRT"
     gene            complement(1629..>3230)
                     /locus_tag="VD791_12985"
     CDS             complement(1629..>3230)
                     /locus_tag="VD791_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08942.1"
                     /translation="DGDGIPRDELGAPDFRYVATHICDSIKRRLKAQGAQGIQGEFGI
                     GLLSFWTVGDELLLTSPGSDGRLYQMRMRKGDPSYTVSQRHVLFGQKGTELKIKPLLP
                     GIRQFSGEKIQWYLASELRDRIRQSGVRVRIVDRQARREYKVEPRQFTGRLLHDLPRA
                     SAPGGEVYVELYLAEPDAENRVGLYRSGTRVLADVAELDAFKRAPWNLGCLQGLMDAP
                     FLNLTPGTRSGIIHDVQYQAFCTAIAPVAARLSEMLEEQRRAEEERATRDILRSIQRA
                     FQEALLALPAEEYDWFDIRGRDAGGALRRRDAEEGVPVESAAGGEEAAAAGGAEAQKQ
                     FFEYAGPLFSVAVAPASCVLPVNRSRTLRAVPRDRIRRLVEEGLAFRWEVAEGQGRLE
                     NAEGEIVTVHAPPEPGLTRLKVTVTQGTVECSAEAMITVTDTLLAESKEPASARQGLP
                     GYTFQRAPGELWRSRYDAAQNVIVVNNGHRDFVYAARSRALKLRYIARLFAKELVLKN
                     FPGHSPEQLLERMIELSLYTEENLR"
BASE COUNT          507 a         1145 c         1083 g          495 t
ORIGIN      
        1 cggcggcaag gacgactggt tctggatcga tatcccggtc ggctatctct acaccatcgg
       61 caacccgcgc accgactggt gctacaagac cgagcccgac ccggggttga acggccgccg
      121 catcggctac gcgcgcggca aggtgttggg tggctgctcc tcgatcaacg ccatgatcta
      181 catgcgcggg cagaagagcg actacgacca ctgggcggcc ctgggcaacc gcggctggtc
      241 ctgggacgag gtgctgccct ggttcaagcg caccgaagac taccagcacg gcgccgacgc
      301 gctgcacggc gcgggcggcg agctgcgggt ggaggagcgg cgggtgaact gggagatcct
      361 cgacgcctgg cgcgaggcgg cggaggaatg cggcatcccc aagatccgcg agttcaaccg
      421 cggcgacaac ttcggcaacg cttacttcca ggtgaaccag cgccggggcg tgcgctggag
      481 cgccaccaag gccttcctgc gtccggtgca gcatcgcccc aacctcgccg tgctcaccgg
      541 cgcgctggcg caccgggtgc gcatcgacac gcacgatggg gtcaagcgcg cgaccggcgt
      601 cgagctctcg ctgtccgacg ggatgcgcac ggtcgaggcg cggcaaggcg tcattctggc
      661 cgccggcgcc atcggctcgc cgcagattct gcagctgtcg ggcgtggggc cggccgcgct
      721 cctgcagcac cacgcgatcc ccgtggtgca cgatctgccc ggcgtcggcg aaaacctcca
      781 cgaccacctg cagatccgcc tgatgtacaa ggtgcacggc gtgaccacgc tcaaccagcg
      841 cgccaactcg ctgctgggca aggccgcgat ggggctggag tacctgttgt tcaagaccgg
      901 cccgctcacc atgccgccct cgcagctcgg cgctttcgcc cgcagcgacc cgtcgcagcc
      961 ctcggcgaac atcgagtggc acgtgcagcc gctgtcgctg gaccggtttg gcgagccgct
     1021 gcacccgttt cccgccatca cgccgtcggt gtgcaacctg cggcccgcca gccgcggcca
     1081 tgtgcgcatc acgagcgccg atccgcgcgc acaccccgcc atcacgctca actacctgtc
     1141 cgcagaggcg gaccggcggg tcgcggtgga ttccatgcgc ttcactcgcc gcatcatggc
     1201 ggccagcgcc ctggcgaagt attcgcccga ggaatggaag ccggggccga gcgcgcaatc
     1261 cgacgaggcg ctgctcaagg ccgccggcga cctcggcacc accatcttcc acccggtggg
     1321 cacctgcaag atgggacacg acgagtttgc cgtggtggac gatcgcctgc gcgtgcgcgg
     1381 catcgaaggg ctgcgcgtga tcgacgcctc ggtcatgccg cgcatcacct ccggcaacac
     1441 caacgcgccg acggtcatga tcgcggagaa gggcgccgcg atgatcgccg aagacttgag
     1501 tcgaacgtag cccggatgga gtgcagcgga atccggggaa gagagaggcc tctttcatcc
     1561 ccggattcag ccgccgttct agtcgacgac gtacttggcg cggctgcatc cgggctacgc
     1621 tcgggcgcct acctaaggtt ctcctcggta tacagcgaca gctcgatcat gcgctcgagc
     1681 agctgctccg gcgagtgccc ggggaagttc ttgagcacca gttccttggc gaatagtcgg
     1741 gcgatgtaac gtagcttgag cgcgcgcgaa cgcgcggcgt acacgaagtc gcggtgacca
     1801 ttgttcacca cgatcacgtt ttgcgccgcg tcgtagcgcg agcgccacag ctcgcccgga
     1861 gcgcgctgga aggtgtagcc gggcagaccc tgcctggcgg aggccggttc cttgctctcg
     1921 gccagcagcg tgtcggtgac cgtgatcatc gcctctgcgc tgcactcgac cgtaccctgc
     1981 gtgaccgtca ccttcagccg ggtgaggccg ggctcaggcg gcgcgtggac ggtcacgatt
     2041 tctccttcgg cgttctccag gcgtccctgg ccctccgcaa cctcccagcg gaacgcgagc
     2101 ccctcctcca ccaggcggcg gatccggtcg cgcggcacgg cccgcagcgt gcgcgaacgg
     2161 ttcaccggta atacgcagga cgccggtgcg acggccacgc tgaacaacgg ccccgcatac
     2221 tcgaagaact gcttctgcgc ttccgcgccg ccggcggccg ccgcctcctc ccctcccgca
     2281 gcgctttcga cgggcacacc ttcctccgca tcgcgtcgtc gcagcgcacc gccggcgtcg
     2341 cggccgcgga tatcgaacca gtcgtactcc tcagcgggca gggcgagcaa ggcctcctgg
     2401 aaggcgcgct ggatcgagcg caggatgtcg cgggtggcgc gctcctcctc ggcccggcgc
     2461 tgctcctcga gcatctcgct gagccgagcg gccaccggcg cgatcgctgt gcagaacgcc
     2521 tggtactgga cgtcgtggat gatgccgctg cgggtgccgg gggtgaggtt caggaacggc
     2581 gcgtccatca ggccctgcaa gcagccaagg ttccagggcg cgcgcttgaa ggcgtcgagc
     2641 tcggcaacgt cggccagcac acgcgtgccg gagcggtaca gcccgacgcg gttctcggcg
     2701 tcgggctcgg ccaggtacag ctcgacgtac acctcgccgc ccggggcgga ggcccgcggc
     2761 aggtcgtgca gcagccggcc ggtgaactgg cgcggctcca ccttgtactc gcgccgggcc
     2821 tggcggtcca cgatcctgac ccttacgccc gattggcgga tgcggtcgcg cagctcggac
     2881 gcgagatacc actggatctt ctcgccgctg aactggcgaa tgccgggcaa cagcggcttg
     2941 atcttgagct cggtcccctt ctgcccgaac agcacgtgac gctgcgacac cgtgtaactg
     3001 gggtcgccct tgcgcatgcg catctgatac agcctgccgt ccgagcccgg cgaggtcagc
     3061 agcagctcgt cgcccacggt ccagaagctc agcagcccga tgccgaactc gccctggatc
     3121 ccctgcgcgc cctgggcctt gagccggcgc ttgatcgagt cgcagatgtg cgttgcgaca
     3181 taacggaagt cgggcgcgcc cagctcatcc cggggaatgc cgtcgccgtc
//
LOCUS       DASPGE010000399         5882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1276112, whole genome shotgun sequence.
ACCESSION   DASPGE010000399 DASPGE010000000
VERSION     DASPGE010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276112"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1824
                     /locus_tag="VD791_12990"
     CDS             169..1824
                     /locus_tag="VD791_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV08943.1"
                     /translation="MFDLVIRGATVYDGSGRPGAPADVAVRDGRIAQVGRVTEPTRKS
                     VDAAGLALMPGIIDLHTHYDAQVTWDPTLSPSPALGVTTAVMGNCGFGIAPCPAPLRD
                     TMLKNLSVVEAMDLQALTTGVRWEFESFAEYMGLLRRARPYANVAVLAGHSCIRTAVM
                     GEEASLRAQPTEAELVRMRELLREAMAEGAIGFASSFSPNHSGYAGRPMPSTIASEEE
                     LRALVGVLGEAGRGVFVMATGARATPDLMESMAADTDRPMFIVTVLTMYNRAEPERST
                     RYYQRVADALDRGREVYIQTSCQPLSFDFTLRDPYPLQSHDAFDRVKSAAPEALPAIY
                     ADPAFRRRFRENLARPRTGILFYGDWDTIELDGASIGARARAAERDPLDFFFDLALEE
                     GLATQFVGKFFQNDDAGVAPLLQHRAGVIGLSDAGAHLIFMCDAGFGLHLLRHWVRET
                     GAFTLQEGVRRLTSDAADKYRIPERGRIAPGAWADLLLFDPATVGVSGLQRAADLPGG
                     GRRMIRRPQGVHGVWVNGVQVHDGRDYVPLEAGPGAVLDRFPA"
     gene            1834..2178
                     /locus_tag="VD791_12995"
     CDS             1834..2178
                     /locus_tag="VD791_12995"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HXV08944.1"
                     /translation="MIAVIFEVWPAEGRAAEYFDLAAALKTELQRIDGFISIERFESL
                     TTRGKYLSLSFWRDEESVRAWRNVEAHRAAQAKGRGGVFADYRLRVASVVRDYGLTER
                     SEAPRDSRGVHG"
     gene            complement(2223..3242)
                     /locus_tag="VD791_13000"
     CDS             complement(2223..3242)
                     /locus_tag="VD791_13000"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HXV08945.1"
                     /translation="MFETTIAGSLPKPAWLAETQRLWPQWKLVGEALNEGKRDATLLW
                     IKAQEDAGIDIVSDGEQSRQHFVHGFLEAVDGIDFAHKVKMGIRNNRYDAMVPVVTGP
                     LRLRGRVHEAETSVARAHTNRRLKITMPGPMTIVDTIADRHYGDKVELAMAFAALLNQ
                     EARALEAQGVDVIQFDEPAFNVYMDDVKRWGIEALHRCIDGLRCRTAVHICYGYGIQA
                     NIDWKKTLGSEWRQYEEIFPALAGSRLDQVSVECIHARVPMELLKLLAGKDVLVGVID
                     VATQTVETPEDVAGVIAEAMKHVPRERLFPCTNCGMAPMSRPVAEAKLAALGAGAALA
                     RSRFA"
     gene            3508..4083
                     /locus_tag="VD791_13005"
     CDS             3508..4083
                     /locus_tag="VD791_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HXV08946.1"
                     /translation="MKTLLTISRAIDALNERIGRVILWLVLVMVLVSSGNAVSRYLLN
                     ISSNAWLELQWYLFAVVFLLCAGYALLHNEHIRIDVVSSHLSRRTQMWIDVFGTIFFL
                     FPVAGTIMWLSWPIFVNAWVSGEMSSNAGGLIRWPARLMIPVGFFLLTLQGVSELIKR
                     VAFLRGLIPDPVEKHVEPALVMVRNDDGAAQ"
     gene            4080..5636
                     /locus_tag="VD791_13010"
     CDS             4080..5636
                     /locus_tag="VD791_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXV08947.1"
                     /translation="MTAFVIENMAPLMFAALVVFLLLGYPVSFALGAVGLAFGWLGVE
                     LGLLHPELFQALPERIFGIMANDTLLAVPFFTFMGLILERSGMAEDLLDTIGQLFGPV
                     RGGLAYAVIFVGALLAATTGVVAASVISMGLISLPIMLRYGYDRRLATGVIAASGTLA
                     QIIPPSLVLIVLADQLGRSVGDMYAGAIFPGLVLTSLYFLYVFGRTLVSPERAPALPA
                     EARTLRGFKLLRRVLFVLMPPLMLIFLVLGTIFLGIATPTEGGAMGATGALILAAINR
                     RLKLSVLKQAMNTTAKLSSFVIFILVGATVFSLTFRAVNGDLWVEHLLTGLPGGQVGF
                     LVVVNILVFVLAFFLDFFELAFIIVPLLAPAADKLGIDLIWFGVLLGINMQTSFMHPP
                     FGFALFYLRSVAPAREYLDKITGKPTAPITTGQIYWGAVPFVVIQCIMVGIVIAFPGL
                     VTANLDRSRVDESTIEINVPATDYGGQEQPVEDSGASELERAFGAPGGADTDAPEESD
                     ATDLEKEFLK"
     gene            complement(5694..>5882)
                     /locus_tag="VD791_13015"
     CDS             complement(5694..>5882)
                     /locus_tag="VD791_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV08948.1"
                     /translation="SVMEACEKATYELYAELMEKSAHWKRIYPAWKKFRDEQFLWFRV
                     AEAAYENYTFNSKLGAGK"
BASE COUNT          816 a         2039 c         1972 g         1055 t
ORIGIN      
        1 cctgccggcg atgctcgccg ccgcgcaagc ggccgccggc atcccgatag ccacggcaac
       61 cgcagtcggc gaggaccacg gcgttctcgc ggccatggcc gactattgcg tccgcagcgg
      121 cgctcggcga acataggagg catttcccaa gtcgggcgaa ggaagagcat gttcgacctc
      181 gtcattcgag gcgccaccgt gtacgacggc agcggtcggc ccggcgcacc ggccgatgta
      241 gcagtgcgcg acgggcgcat cgcgcaggtg gggcgcgtga ccgaacctac gcgcaagagt
      301 gtcgacgctg cggggctggc gctcatgccc ggcatcatcg atctgcacac gcactacgac
      361 gcccaggtca cctgggaccc gacgctgtcg ccgtcgcccg cgctcggcgt gaccaccgcg
      421 gtgatgggca actgcggctt cggcatcgcg ccctgccccg cgccgctgcg cgacaccatg
      481 ctgaagaacc tgtccgtggt ggaagccatg gacctgcaag cccttacgac cggggtgcgc
      541 tgggagttcg agtctttcgc cgaatacatg gggctgctgc gccgcgcccg gccgtacgcc
      601 aacgtcgccg tgctggcggg gcattcctgc atccgcactg cggtgatggg cgaggaagcg
      661 tcgctgcgtg cgcaaccgac cgaagccgaa ctggtgcgga tgcgcgagtt gctgcgcgag
      721 gcgatggcgg agggtgcgat cggcttcgcc tcgtcgttct ccccgaatca ttccggctat
      781 gccggccggc cgatgccctc caccatcgcc tccgaggagg agctgcgtgc gctggttggc
      841 gtgctgggcg aggctggccg cggcgtgttc gtgatggcca ccggtgcccg cgccacgccc
      901 gacctgatgg aatcgatggc cgccgacacc gaccggccca tgttcatcgt caccgtgctc
      961 accatgtaca accgggccga gcctgagcgc tccacgcgct attaccagcg cgtggccgat
     1021 gccctcgatc gtggccgcga ggtgtacatc cagaccagct gccagccgct gtcgttcgac
     1081 ttcacgctgc gggatcccta tccgctgcag tcgcacgacg ctttcgaccg cgtgaagagc
     1141 gcagcgcccg aggcgctgcc cgccatctat gccgatccgg cgtttcgccg gcgcttccgc
     1201 gagaacctgg cgcggccgcg caccggcatc ctgttctacg gcgactggga cacgatcgag
     1261 ctggacggcg ccagcatcgg cgcccgggcg cgcgcggccg agcgcgaccc gctcgacttc
     1321 ttcttcgatc tcgccctgga ggaaggcctt gccacgcagt tcgtgggcaa gttcttccag
     1381 aacgacgacg ccggagtggc gccgctgctg cagcaccgtg ccggcgtcat cgggctgtcg
     1441 gacgccggcg cgcacctcat cttcatgtgc gacgccgggt tcgggctgca cttgctgcgc
     1501 cactgggtgc gcgagaccgg cgcgttcacg ctgcaggaag gcgtgcgccg cctgaccagc
     1561 gacgccgccg acaagtaccg cattcccgag cgcggccgca tcgcgcccgg cgcatgggcc
     1621 gacttgctgt tgttcgaccc ggcgacggtg ggcgtttcgg gcctgcagcg cgcggccgac
     1681 ctgcccggcg gcggcaggcg catgatccgc cgcccgcagg gcgtgcacgg cgtgtgggtg
     1741 aacggcgtgc aggtgcacga cgggcgcgac tatgttccgc tcgaggccgg gcccggtgcg
     1801 gtgctcgacc ggtttcctgc gtaggagcgc acagtgatag ccgtgatctt cgaggtctgg
     1861 ccggccgagg gcagggctgc ggaatatttc gatctcgccg ccgccctcaa gaccgagctg
     1921 caacgcatcg acgggttcat ctccatcgag cgcttcgaga gcctgaccac gcggggcaag
     1981 tacttgtcgc tgtccttctg gcgcgacgag gagagcgtgc gcgcatggcg caacgtcgaa
     2041 gcgcatcgag ccgcccaggc caaggggcgc ggcggcgtgt tcgcggatta ccgcttgcgc
     2101 gtggcctcgg tggtgcgcga ctacgggctc accgagcgca gcgaagcgcc gcgggacagc
     2161 cgcggcgtgc acggttagcg cgccccgtca cccctcactc ccgccgggtg agaggggagt
     2221 tgctacgcga accggctgcg cgccagcgcc gcgccggcgc cgagcgccgc gagcttggcc
     2281 tcggccaccg ggcgcgacat cggcgccatg ccgcaattgg tgcaggggaa caagcgctcc
     2341 ctgggcacgt gtttcatcgc ctcggcgatc acaccggcga cgtcctccgg cgtctcgacc
     2401 gtctgcgtgg ccacgtcgat cacgcccacc agcacgtcct taccggccag cagcttcagc
     2461 aactccatcg gcacgcgcgc gtggatgcac tccaccgaca cctggtcgag gcggctgccc
     2521 gccagcgccg gaaagatctc ttcgtactgc cgccattcgc tcccgagcgt cttcttccag
     2581 tcgatgttcg cctggatgcc gtagccgtag cagatgtgca cagcggtgcg gcagcgcagc
     2641 ccgtcgatgc agcggtgcag cgcctcgatc ccccagcgct tcacgtcgtc catgtagacg
     2701 ttgaaggcgg gctcgtcgaa ctggatcacg tccacgccct gcgcttcgag cgcgcgcgcc
     2761 tcctggttga gcaacgcggc gaaggccatc gccagctcga ccttgtcgcc gtagtgccgg
     2821 tcggcgatgg tatcgacgat ggtcatgggc ccgggcatgg tgatcttgag ccgcctgttc
     2881 gtgtgcgctc gcgccacgct ggtttcggct tcatggaccc ggccgcgcag tcgcaggggg
     2941 ccggtcacca ccggcaccat ggcgtcgtag cggttgttgc ggatgcccat cttcaccttg
     3001 tgcgcgaaat cgatgccgtc gaccgcttcc aggaagccgt gcacgaagtg ctgccgcgat
     3061 tgctcgccgt cgctgacgat gtcgatcccg gcgtcctcct gcgccttgat ccagagcaag
     3121 gtggcgtcgc gcttgccctc gttgagcgcc tctccgacga gcttccactg cggccagagc
     3181 cgctgcgtct ccgcgagcca ggccggcttg ggcaggctgc ctgcgatggt agtttcgaac
     3241 atgttgggcg gtgtccggac gagtggtggc ggcgcacggc ggcggtcgag gatgcggacg
     3301 atcctcggcg gcatgcctgc gcagtgtcat gatagcgcgc ctgggtgccg ggcactggtg
     3361 ctctcgagca cgggggctcc ctggggccag cccttggcgc ccctgcgcgc gcttttgcat
     3421 ccgccccgcc aatcggcgac aatccacgcg gccctgcatc cgctcagggc gtgatccgag
     3481 cagttccgag gggaggagcc cggcgccatg aaaacgctgc tgaccatctc gcgcgccatc
     3541 gacgcgctca acgagcgcat cgggcgggtg atcctgtggc tggtgctggt gatggtgctg
     3601 gtcagctcgg gcaacgccgt ctcccgctac ctgctgaaca tcagctccaa cgcctggctg
     3661 gagctgcagt ggtacctgtt cgcggtcgtg ttcctgctgt gcgccggcta cgccctgctg
     3721 cacaacgagc acatccgcat cgatgtcgtg tcctcgcacc tgtcgcgccg cacccagatg
     3781 tggatcgacg tgttcggcac gatcttcttc ctgtttccgg tggccggcac catcatgtgg
     3841 ctgtcctggc cgatcttcgt caacgcctgg gtgagcggcg agatgtccag caacgccggc
     3901 ggcctgatcc gctggccggc gcgcttgatg atcccggtcg gcttcttcct gctcacgctg
     3961 cagggcgtct cggagctgat caagcgcgtg gccttcctgc gcggcctgat ccccgacccg
     4021 gtggagaagc acgtcgagcc ggcgctggtg atggtccgca acgacgacgg ggccgcgcaa
     4081 tgaccgcctt cgtcatcgag aacatggcgc cgctgatgtt cgcggcgctg gtcgtattcc
     4141 tgctgctcgg ctacccggtt tcctttgcgc tcggcgcggt cggcctggcg ttcggctggc
     4201 tcggcgtcga gctggggttg ctgcaccccg agctattcca ggcgctgccc gagcggattt
     4261 tcgggatcat ggccaacgac acgctgctcg cggtgccgtt cttcaccttc atggggctga
     4321 tcctggagcg cagcggcatg gccgaggacc tgctcgatac gatcggccag ctgttcggcc
     4381 cggtgcgcgg cgggctcgcc tacgcggtga tcttcgtcgg cgcgctgctg gcggcgacca
     4441 ccggcgtggt cgccgcgtcg gtcatctcca tgggtctgat ctcgctgccc atcatgctgc
     4501 gctacggcta cgaccggcgg ctcgccaccg gcgtgatcgc ggcttccggc acgctggcac
     4561 agatcatccc gccctctctc gtgctgatcg tgctggccga tcaactcggc cgctcggtcg
     4621 gagacatgta cgcgggcgcg atcttccccg gactggtgct gaccagtctg tattttctct
     4681 atgtcttcgg ccgcaccctc gtctcacccg agcgcgcccc ggcgctgccg gccgaggcgc
     4741 gcacgctgcg cggcttcaag ctcctgcggc gcgtgctgtt cgtgctgatg ccgccgctca
     4801 tgctgatctt cctggtgctg ggcacgatct tcctgggcat cgccacgccg accgaaggcg
     4861 gggccatggg tgcgaccggc gcgctgatcc tggcggccat caaccggcgg ctcaagctgt
     4921 ccgtgctcaa gcaggcgatg aacaccacgg cgaagctgtc ctcgttcgtg atcttcatcc
     4981 tggtcggcgc caccgtgttc agcctgactt tccgcgccgt gaacggcgat ctgtgggtcg
     5041 agcacctgct gaccggcctg cccggcggtc aggtcggctt cctggtggtg gtcaacatcc
     5101 tggtcttcgt gctcgccttt ttcctcgact tcttcgagct ggcgttcatc atcgtgccgc
     5161 tgctcgcgcc ggccgccgac aagctcggca tcgacctgat ctggttcggg gtcctgctcg
     5221 ggatcaacat gcagacctcg ttcatgcacc cgccgttcgg cttcgcgctg ttctatctgc
     5281 gcagcgtcgc gcccgcccgg gagtatctgg acaagatcac cggcaagccg accgccccga
     5341 tcaccaccgg ccagatctac tggggcgcgg tcccgttcgt ggtgatccag tgcatcatgg
     5401 tcggcatcgt catcgcgttc ccgggcctgg tgaccgcgaa cctagaccgc agcagggtcg
     5461 acgagtcgac catcgagatc aacgtcccgg cgaccgatta tggcggccag gagcagccgg
     5521 tggaggattc cggggcgagc gagctggaga gagccttcgg cgcgccgggc ggcgccgaca
     5581 cggacgcgcc cgaagaatcg gacgccaccg acctcgagaa ggagttcctg aagtaggttc
     5641 cgacgagcag cgaagagccc cgcgtacgct gggctcttcc caatcagtag gccctacttg
     5701 cccgcgccca gcttgctgtt gaacgtgtag ttctcgtagg ccgcttccgc gacgcggaac
     5761 cagaggaact gctcgtcgcg gaatttcttc caggcggggt agatgcgctt ccagtgcgcg
     5821 ctcttctcca tcagttcggc gtacagctcg taggtggcct tctcgcaggc ctccatcacc
     5881 ga
//
LOCUS       DASPGE010000400         9262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1628428, whole genome shotgun sequence.
ACCESSION   DASPGE010000400 DASPGE010000000
VERSION     DASPGE010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9262
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628428"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VD791_13020"
     CDS             complement(<1..959)
                     /locus_tag="VD791_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXV08949.1"
                     /translation="MSSYKAFHRRSIEDREGFWAEQARLVHWHKPAAKVLDYSRPPFA
                     RWFVGGETNLCYNAVDRHLTARADQKALVFISTETGDERSYTFRDLHAEVTRFAAVLR
                     GLGVGHGDRVIIYMPMIPEACFAMLACARIGAIHSVVFGGFASHSLASRIDDARPKLI
                     VSSDAGMRAGKAVPYKHLLDEAIRLSAARPQKVLLVDRGIDREMPRTPGRDVDYAALH
                     AQHLSAEVPIVWLESNEPSYILYTSGTTGKPKGVQRDVGGYAVALAASMRYIFCGEAG
                     ETMFTTSDIGWVVGHSYIIYAPLIAGMTTVMYEGVPIRPDPGI"
     gene            complement(1046..2038)
                     /locus_tag="VD791_13025"
     CDS             complement(1046..2038)
                     /locus_tag="VD791_13025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HXV08950.1"
                     /translation="MAKFKAFRIFSEDGKTVARLVEMDLDELDPGEVVIKTAYSSINY
                     KDALAATGAGRIIRRFPCVGGIDGCGTVVSSTDARFKAGDPVIATSYDLGVAHDGGYA
                     ECMRVKADWVVPLPKGLSLFEAMALGTAGYTAGLAVVRMEHEGLAPANGPVIVTGATG
                     GVGSVAVDILAGAGYHVVALTGKDAESDYLKRLGAKEIVLRSALDLAKIKPLDKATWA
                     GAVDNLGGDVLSWLASTMQVGGALASVGLAAGHAFNTTVMPFILRGVSLLGVDSVNTP
                     MPVRQRVWERLAGDLKPRHLESMVTTVGLVGMQPVFEKILKAQFRGRTVVKIGA"
     gene            complement(2170..3255)
                     /gene="zapE"
                     /locus_tag="VD791_13030"
     CDS             complement(2170..3255)
                     /gene="zapE"
                     /locus_tag="VD791_13030"
                     /inference="COORDINATES: protein motif:HMM:NF015902.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HXV08951.1"
                     /translation="MSPGFVPRLREVAHEHGFALDAAQERVAAEFQRLEGELAAAERD
                     GSGLRRWLRRRSAVKGIYLWGPVGRGKSFLMDAFFRLTRIERKRRVHFHRFMQDIHHR
                     LRAVQGQRDPLRAVARDTGRGVRLLCLDELHVADIGDAMLMRGLLERLFERGVVLVTT
                     SNQRPDELYLHGLQRAQFLPAIELMKQHLEVVELSGGSDYRLRTLEKAGVYHFPLGEP
                     AEAAQKAAFLGVAGEAGEADVALEIDGRRILARHVGAGTVWFGFAELCEEPRGTADYI
                     ELARRYHTVLISGVPKFRPSMAESLRRFTWLVDEFYDRRVKLILSADAPLEALCQDIE
                     PRPDIERTRSRLIEMQTSRYLSAAHLA"
     gene            complement(3255..4685)
                     /gene="lpdA"
                     /locus_tag="VD791_13035"
     CDS             complement(3255..4685)
                     /gene="lpdA"
                     /locus_tag="VD791_13035"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXV08952.1"
                     /translation="MAQQFDVAVIGAGPGGYTAAIRAAQLGLKTVCIDDWKNARGEPS
                     LGGTCLNVGCIPSKAMLESSENYERVRDKFAEHGIKAQGVTVDLAAMLARKDKIVDTF
                     TGGIALLFKKNKVASLHGHGSFLAGRGPYRIQVKDGEHVEEVEARHVIIATGSVAREL
                     PGAPFDSERILDNVGALTLPEIPRRLGVVGAGVIGLEMGSVWRRLGAEVIILEALPAF
                     LPAADEQVAKEAFRVFNGPLDLKIYTGVNITAVKSGAKAVSLTYTDRDGNTRMADFDR
                     LIVAIGRVPNTAGLRADAVGLKIDARGFVEVDARNRTNLPDVYAIGDVVRGPMLAHKS
                     SEEGVAVAETIAGEAGHVNLDTIPWVIYTSPEIAWVGKTEQQLKADGTPYRAGRFPFI
                     ASGRARALGETAGFVKMLAHDESDRILGVHAIGPYASELIAEAVVAMEFGASAEDIAR
                     VVHAHPTLSEAMHEAALGVHKRTLNI"
     gene            complement(4837..6075)
                     /gene="odhB"
                     /locus_tag="VD791_13040"
     CDS             complement(4837..6075)
                     /gene="odhB"
                     /locus_tag="VD791_13040"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HXV08953.1"
                     /translation="MIEVRVPQLSESVSEATLLTWHKKPGEFVERDENLIDIETDKVV
                     LELPAPESGVLAKIVKHDGGTVVSNEVIATIDTEAKAAAAAAAKAATTAAATGAAAKT
                     AAVRAPEAPAAKGAAQVGIMPAARKLATEKGVDLSVVEGTGRGGRITKEDVAAQLARP
                     AAAKPAIAPPAADIGQLLGDRPEQRVPMSRLRQRVAERLLQSQSTAAILTTFNEVNMQ
                     PVIELRRKYQERFEKEHGVRLGFMSFFVKASVHALKKFPVVNASVDGTDIVYHGYFDI
                     GIAVASPRGLVVPILRNADQLSLAEMEKQIGDFGKRAQEGKLGIEELTGGTFSISNGG
                     VFGSMLSTPIINPPQSAILGIHLTKDRPVVEDGQIVIRPMNYLALSYDHRIIDGREAV
                     LSLVAIKEALEDPARMLLDL"
     gene            complement(6186..9008)
                     /locus_tag="VD791_13045"
     CDS             complement(6186..9008)
                     /locus_tag="VD791_13045"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004526855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HXV08954.1"
                     /translation="MAALLKEYLGTSYLFGANAPFIEELYEAYLADPASVAPQWRRYF
                     DQLQQLPGAPDVAHEPIRQSFVKLVKAPRTGIGHAATASVAAAEKKQVKVLQLTNNYR
                     RLGLRHANVDPLKRFEKPPVPELDPAFYGLTEEDMELVFNTAADFGPEQMPLREIVRA
                     LRETYCGTIGAEFWFMADSAQRRWIRQRLESIRSQPSYSADFKRFILERLTAAETLEK
                     YLHTRYVGQKRFSVEGGDTLITLLDHLLQTAGEKGVQETVLGMAHRGRLNVLVNTMGK
                     MPKDLFLEFEGKNVDHLPAGDVKYHMGFSSDLNTPGGPMHLSLAFNPSHLELVNPVVE
                     GSVRARQHRRKDREGRQVLPVLIHGDAAFAGQGVVMETLNLSQTRGYRTGGTLHIIVN
                     NQIGFTTSDVRDVRSTLYCTDVVKMVEAPVFHVNADDPEAVCLVTEIALDFRMQFQKD
                     VVIDLVCFRRHGHNEQDEPMVTQPLMYKLISQHPGTRKLYADRLAAQGVVRPEEAEDM
                     IKAYRSALDQGYHTNKNVLTSFHPPYAVNWERFRGAKWTDPADTSVPLPELKRLGERL
                     TTIPPEFKLHPRVEKVIADRRQMMDGKIPFDWGMAENLAYAAVLRDGFGVRLSGQDTG
                     RGTFFHRHAVLHDQARERWDAGAYVPLQHIAENQPPFVVIDSVLSEEAVMGFEYGYAT
                     AEPNDLVVWEAQFGDFANGAQVVIDQFIASGEVKWGRLCGLTLMLPHGYEGQGPEHSS
                     ARLERFLQLCADYNMQIVVPSTPAQMFHVLRRQMLRPFRKPLIIMSPKSMLRHKESVS
                     SLEDLAEGRFQPVIGETDDIDPSQVRRVVFCSGRMYYDLRAARRERGAKDIAIVRLEQ
                     LYPFPHDDYTGQIQKYAGAKDVIWCQEEPGNQGAWHRIQHYLLRHMLPHQKLAYALRP
                     SAASPAVGYMQLHQEQQKAVIEAALG"
     gene            complement(9079..>9262)
                     /locus_tag="VD791_13050"
     CDS             complement(9079..>9262)
                     /locus_tag="VD791_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HXV08955.1"
                     /translation="QRAMGIPTSMFTAIFALARTVGWVAQWNEMIADPEQKIGRPRQL
                     FTGSPERDVPDLTMRR"
BASE COUNT         1429 a         3188 c         3050 g         1595 t
ORIGIN      
        1 cagatgccgg ggtcggggcg gatcggcacg ccctcgtaca tcaccgtggt catgccggcg
       61 atcagcggcg cgtagatgat gtaggagtgc ccgaccaccc acccgatgtc cgaagtggtg
      121 aacatggttt cgccggcctc gccgcagaag atgtatcgca tcgacgccgc cagcgccacc
      181 gcgtagccgc ccacgtcgcg ctgcacgccc ttcggcttgc cggtggtgcc cgaggtgtaa
      241 aggatgtacg acggctcgtt cgactccagc cacacgatcg gcacctcggc gctcaggtgc
      301 tgggcgtgca gcgcggcgta atccacgtcg cggcccggcg tgcgcggcat ctcgcgatcg
      361 atcccgcggt ctaccagcag cactttctgc ggccgggccg ccgacaggcg gatggcttcg
      421 tccagcaagt gcttgtaggg caccgctttc ccggcgcgca tgccggcgtc ggacgacacg
      481 atcagcttcg gccgcgcatc gtcgatgcgc gaggccaggc tgtgcgaggc aaatccgccg
      541 aacaccaccg agtggatcgc cccgatgcgg gcgcaggcga gcatcgcgaa gcacgcttcg
      601 gggatcatcg gcatgtagat gatcacgcgg tcgccgtggc ccacgcccag gccgcgcagc
      661 acggcggcga accgggtcac ctccgcgtgc aggtcgcgga aggtgtagga gcgctcgtcc
      721 cccgtctccg tggagataaa taccagcgcc ttctgatcgg cgcgcgcggt aagatgccgg
      781 tccaccgcgt tgtagcagag attggtctcg ccgccgacga accagcgggc gaacggcggc
      841 cggctgtaat ccagcacctt ggcggcgggc ttgtgccagt ggaccaggcg cgcctgctcc
      901 gcccagaagc cttcccggtc ctcgatcgac cggcgatgga acgccttgta gctgctcacg
      961 ctgctgctcc tcctcctccg cctctcccct ggcgctgcgc ttctccgact tggggcgacg
     1021 ccacccgcgc gcaggttccg ccggctcaag cgccgatctt caccacggtg cggccccgga
     1081 actgcgcctt caggatcttc tcgaacaccg gctgcatgcc caccaggccc accgtggtca
     1141 ccatggactc gaggtgccgc ggcttgaggt cgcccgccag gcgctcccag acgcgctggc
     1201 gcaccggcat cggggtgttc accgaatcta cgcccagcag gctcacgccc cgcaggatga
     1261 acggcatcac cgtcgtgttg aaggcatggc cggcggccag gccgacgctg gctagcgcgc
     1321 cgccgacctg catggtgctc gccagccagg acagcacgtc gccccccagg ttgtccaccg
     1381 cgccggccca ggtggccttg tcgagcggct tgatcttggc gagatcgagc gcgctgcgca
     1441 gcacgatctc cttggcgccg aggcgcttga gatagtcgct ttcggcgtcc ttgccggtga
     1501 gcgcgaccac gtgatagccg gcgccggcca ggatgtcgac ggcgacgctg cccacgccgc
     1561 cggtggcgcc ggtcacgatc accgggccgt tggccggcgc gaggccctcg tgctccattc
     1621 gcaccaccgc aaggccggcg gtgtagccgg cggtgcccag cgccatcgct tcgaacaggc
     1681 tgaggccctt gggcagcggc accacccaat cggccttcac gcgcatgcat tccgcgtagc
     1741 cgccgtcgtg cgccacaccg agatcgtaag aggtcgcgat gaccggatcg ccggccttga
     1801 agcgggcgtc ggtcgaggac accacggtcc cgcagccgtc gatgccgcca acgcagggaa
     1861 agcggcggat gatccggccg gcgccggtcg cggccagcgc atctttgtag ttgatgctcg
     1921 agtacgcggt cttgatcacc acctcgcccg gatccagctc atcgagatcc atctcgacaa
     1981 gccgcgcgac cgtcttaccg tcctcgctga agatccggaa cgccttgaac ttggccatga
     2041 tctgctattc ctcccgtcgt tcgcgacgct gacccgtcgg tatgttaccg ccgtacgcag
     2101 tcgctgtgat gcctcccgcg cctcggatga ttacgatgtg ctcggtcgtt gacctgccgg
     2161 agatgggcat caagccaggt gcgccgcgct caagtaccgg ctcgtctgca tctcgatcag
     2221 gcggctgcgc gtgcgctcga tatccgggcg gggctcgata tcctggcaca gggcttcgag
     2281 cggcgcgtcc gccgacagga tcagcttcac ccggcggtcg tagaactcgt ccaccagcca
     2341 ggtgaagcgg cgcaggctct ccgccatgga cggccggaac ttcggcacgc ctgagatcag
     2401 caccgtatgg tagcgccgcg cgagctcgat gtaatcggcg gtgccgcgcg gttcctcgca
     2461 cagctccgcg aagccgaacc agaccgtccc cgcccccaca tgccgggcca ggatcctgcg
     2521 gccgtcgatc tccagcgcta cgtcggcctc cccggcctcg cccgcgaccc cgaggaacgc
     2581 cgccttctgc gccgcctcag cgggttcacc cagcggaaag tggtacacgc cggccttttc
     2641 cagtgtgcgc aggcggtaat cgctgccgcc cgatagctcg acgacttcga ggtgctgctt
     2701 catcagctcg atcgcgggga ggaactgggc gcgttgcagg ccgtgcaggt acagctcgtc
     2761 cggacgctgg ttggaggtgg taaccagtac cacgccccgc tcgaacaacc gctccagcag
     2821 ccctcgcatc agcatggcgt cgccgatgtc ggcgacgtgc agctcgtcca ggcacagcag
     2881 gcgcacgccg cgcccggtgt cccgggccac cgcgcgcagc gggtcgcgct gcccctgcac
     2941 agcgcgcaag cggtggtgga tgtcttgcat gaagcgatgg aagtggacgc ggcgcttgcg
     3001 ctcgatccgc gtgaggcgaa agaaggcgtc catcaggaag ctcttgcccc ggccgaccgg
     3061 cccccacaga tagatgccct tcaccgctga ccgacgccgc aaccagcgcc gcaggccgga
     3121 gccgtcgcgc tcggccgccg ccagttcgcc ttccaggcgt tggaattcgg cggctacgcg
     3181 ttcctgcgcg gcatcgagcg cgaagccatg ctcgtgagcg acctcgcgca gccggggcac
     3241 gaaccccggg ctcatcagat attcagcgtt cgcttgtgca cgcccagagc ggcttcgtgc
     3301 atcgcttcgg acagcgtcgg atgggcatgc accacgcggg cgatgtcttc ggcgctggcg
     3361 ccgaactcca tcgccaccac cgcctcggcg atcagctcgg aagcgtaggg accgatggcg
     3421 tgcacgccca ggatgcggtc gctctcgtcg tgggccagca tcttgacgaa gcccgcggtc
     3481 tcccccagcg cccgcgcgcg accgctggcg atgaaaggaa agcgcccagc gcggtacggc
     3541 gtgccgtcgg ccttgagctg ctgctcggtc ttgccgaccc aggcgatctc cggcgaggtg
     3601 tagatcaccc agggaatcgt gtcgaggttg acgtggccgg cctcaccggc gatcgtctcg
     3661 gccaccgcaa cgccctcctc cgaggacttg tgggccagca tcgggccacg caccacgtcg
     3721 ccgatggcgt acacgtcggg gaggttggtg cggttgcgag cgtccacctc cacgaagccg
     3781 cgcgcatcga tcttcaagcc cacggcatcc gcgcgcaggc cggccgtgtt ggggactctt
     3841 ccgatcgcca cgatcaggcg gtcgaaatcc gccatgcggg tattgccgtc gcggtcggta
     3901 taggtgagcg aaaccgcctt ggcgccgctc ttgaccgctg tgatgttcac gccggtatag
     3961 atcttgaggt cgagcgggcc gttgaagacg cggaaggctt ccttggccac ctgctcgtcg
     4021 gcggccggca gaaacgccgg cagcgcttcg aggatgatga cctcggcgcc cagacggcgc
     4081 cacacgctgc ccatctccag cccgatcacg ccggcgccga ccacgcccag gcgcctgggg
     4141 atttcgggga gcgtcagggc accgacgtta tccaggatgc gctcgctgtc gaacggcgcg
     4201 cccggcagct cgcgcgccac cgagccggtg gcgatgatca catgcctggc ctcgacctcc
     4261 tcgacatgct ctccatcctt gacctgaatg cggtagggtc ccctcccggc caggaacgac
     4321 ccgtgcccgt gcaaagaggc gaccttgttc ttcttgaata gcagcgcgat gccaccggtg
     4381 aaggtgtcga cgatcttgtc cttgcgcgcc agcatggccg ccaggtccac cgtcacgccc
     4441 tgcgccttga tgccgtgctc ggcgaacttg tcgcgcaccc gctcgtagtt ctcggaggac
     4501 tcgagcatcg ccttggacgg gatgcagccg acgttgaggc aggtgccgcc caggctcggc
     4561 tccccccggg cgttcttcca gtcgtcgatg cacaccgtct tcaggccgag ctgggcggcg
     4621 cggatggcgg cggtgtaccc gcccggtccg gcaccgatca ctgcgacgtc gaattgttgg
     4681 gccatgttcg aggggctagg atctaggagc taggatctag gggcgaggat cgagtcggga
     4741 gatgatggtc aagggtcgag gctcgcctgc aggaaattgc agcagccgtc tccctgcctc
     4801 ggagtcacta gatcctagct cctagatcct agatcctcac aggtccagca gcatccgcgc
     4861 cgggtcttcc agcgcctcct tgatcgcgac cagcgacagc accgcctcgc gcccgtcgat
     4921 gatgcgatgg tcgtaggaca gggccagata gttcatcggc cggatcacga tttggccgtc
     4981 ttcgaccacc ggccggtcct tggtgagatg aatgccgagg atggcgctct gcggcgggtt
     5041 gatgatcggc gtggacagca ttgagccgaa cacgccgccg ttggagatgg agaaagtgcc
     5101 gccggtgagc tcctcgatgc cgagcttgcc ctcctgcgcg cgcttgccga agtcgccgat
     5161 ctgcttctcc atctcggcga gcgagagctg gtcggcgttg cgcaagatcg gcaccaccag
     5221 gccgcgcggg ctcgccaccg cgatgccgat gtcgaagtag ccgtgataga cgatgtcggt
     5281 gccgtccacc gacgcgttga ccaccgggaa cttcttgagc gcgtgcaccg aagccttcac
     5341 gaagaaagac atgaagccca ggcgcacgcc gtgttccttt tcgaagcgtt cctgatactt
     5401 ccggcgcagc tcgatcaccg gctgcatgtt cacttcgttg aaggtggtga gaatggccgc
     5461 ggtcgactgc gactgcagca ggcgctccgc gacgcgctgg cgcaagcgcg acatcggcac
     5521 gcgttgctcg ggccggtcgc ccagcagctg gccgatatcc gccgcgggcg gcgcgatggc
     5581 gggcttggcc gcggccggcc gcgcgagctg cgccgccacg tcttccttgg tgatgcgccc
     5641 gccgcggccc gtcccctcga cgaccgacag atccacgccc ttctccgtcg ccagcttgcg
     5701 cgcagccggc atgatgccca cctgcgccgc acccttggcg gccggcgcct cgggtgcacg
     5761 caccgcagcg gtcttggccg cggccccggt ggccgccgcc gtggtggccg ccttggccgc
     5821 cgccgccgcg gcagccttcg cctctgtgtc gatggtggcg atgacctcgt tgctgaccac
     5881 cgtccccccg tcgtgcttga cgatcttcgc cagcacgccg ctttcgggcg cgggcagctc
     5941 gagcaccact ttgtcggtct cgatgtcgat caggttctca tcccgctcca cgaactcgcc
     6001 cggcttcttg tgccaggtga ggagcgtcgc ttcggagacc gactccgaaa gctgtgggac
     6061 tctgacttca atcaacatgg gcctgggcgg cctggtgcgc ccttaaggcg ggaatcaaag
     6121 aaacccgcac cggaagccgg ttcatccgca taagctcctg tcccatctgc acggcatatt
     6181 caccctcacc caagggcggc ctcgattacg gctttttgct gctcctggtg caactgcatg
     6241 taacccaccg ccggcgatgc ggccgacgga cgcaaggcgt aggccagctt ctggtgcggc
     6301 agcatgtgac gcaacaggta gtgctggatg cgatgccagg cgccctggtt gcccggctcc
     6361 tcctggcacc agatcacatc tttggcgccg gcgtatttct ggatctgccc ggtgtagtcg
     6421 tcgtgcggga acgggtagag ctgctccagc cggacgatgg cgatgtcctt cgcgccgcgc
     6481 tcgcgccgcg ccgcgcgcaa gtcgtagtac atccggccac tgcagaacac cacgcgccgc
     6541 acctgcgacg gatcgatgtc gtcggtttcc ccgattacgg gctggaagcg cccttcggcg
     6601 agatcctcca ggctggaaac cgattccttg tggcgcagca tgctcttcgg gctcatgatg
     6661 atgagcggct tgcggaacgg ccgcagcatc tgccgccgca gcacatggaa catctgcgcc
     6721 ggggtggagg gcaccacgat ctgcatgttg tagtcagcgc acagctgcag gaagcgctcc
     6781 aggcgcgcgg aggagtgctc cggcccctgc ccctcgtagc cgtggggcag catcagcgtg
     6841 agcccgcaca ggcggcccca tttgacttcg cccgaggcga tgaactggtc gatcaccact
     6901 tgcgcgccgt tggcgaaatc gccgaactgc gcctcccaca ccaccagatc gttgggctcg
     6961 gcggtggcat agccgtactc gaagcccatc accgcctcct cggacagcac cgagtcgatc
     7021 accacgaacg gcggctggtt ctcggcgatg tgctgcagcg gaacataggc acccgcgtcc
     7081 cagcgttcgc gcgcctggtc gtgcagtacc gcgtgtcgat ggaagaaggt gccgcggccg
     7141 gtgtcctgcc cggacagacg cacgccgaag ccgtcgcgga gcacggccgc ataggccagg
     7201 ttctcggcca taccccagtc gaacgggatc ttgccgtcca tcatctggcg ccggtcggcg
     7261 atgactttct ccacgcgcgg gtgcagcttg aactcgggcg ggatagtggt gagccgctcc
     7321 cccagccgct tgagctcggg cagcggcacg ctggtatcgg ccgggtcggt ccacttggcg
     7381 ccgcggaagc gttcccagtt caccgcgtac ggagggtgaa agctggtgag cacgttcttg
     7441 ttggtgtgat aaccctggtc cagcgcagag cggtacgcct tgatcatgtc ctcggcttcc
     7501 tcggggcgca ctacgccctg cgcggcgagc cggtcggcgt agagcttgcg cgtgccggga
     7561 tgctggctga tcagcttgta catcagcggc tgggtgacca tcggctcgtc ctgctcgttg
     7621 tggccgtggc gccggaagca gaccaggtcg atgaccacgt ccttctggaa ctgcatgcgg
     7681 aagtcgagcg cgatctcggt caccaggcac accgcctcgg gatcgtcggc attgacgtgg
     7741 aacaccggcg cttccaccat cttcaccaca tcggtgcagt acagcgtgga gcgcacatcc
     7801 cgcacgtccg aggtggtgaa gccgatctgg ttgttgacga tgatgtgcag cgtgccgccg
     7861 gtacgatagc cgcgcgtttg cgacaggttg agcgtctcca tcaccacgcc ctgcccggcg
     7921 aaggcggcgt cgccgtggat cagcaccggc agcacctgcc ggccctcgcg atccttgcgc
     7981 cggtgctggc gggcgcgcac cgagccctcc accaccgggt tgacgagctc caggtgcgac
     8041 ggattgaagg cgagcgacag gtgcatcggg ccgcccggcg tgttgaggtc ggacgagaag
     8101 cccatgtggt acttcacgtc gccggccggc aagtgatcga cgttcttgcc ctcgaactcc
     8161 aggaacaggt ccttgggcat cttgcccatg gtgttgacca gcacgttgag ccggccgcgg
     8221 tgggccatgc cgagcaccgt ttcctgtacg cccttctcgc ccgccgtctg cagcaggtgg
     8281 tcgagcagcg tgatgagcgt gtcgccgcct tcgaccgaga agcgtttctg gccgacgtag
     8341 cgcgtgtgca agtacttttc cagggtctcg gcggcggtga ggcgctccag gatgaagcgc
     8401 ttgaagtcgg ccgagtagct gggctgcgac cggatgctct ccagccgctg gcggatccag
     8461 cgccgctgcg cgctgtccgc catgaaccag aactccgcgc cgatggtgcc gcaatacgtc
     8521 tcgcgcagcg cgcgcacgat ctcgcgcagc ggcatctgct cgggcccgaa gtcggcggcg
     8581 gtgttgaaca ccagctccat gtcctcttcg gtcaacccgt agaacgccgg gtcgagctcc
     8641 ggcaccggcg gtttctcgaa gcgtttgagc ggatcgacgt tggcgtgccg caaccccagg
     8701 cgccggtaat tgttggtgag ctgcagcacc ttcacctgct tcttctccgc cgccgcgacc
     8761 gaggcggtgg cggcgtggcc gatgccggtg cgcggggcct tcaccagctt cacgaacgac
     8821 tggcggatcg gttcgtgcgc cacgtccggc gccccgggaa gctgctgcag ctggtcgaaa
     8881 tagcgccgcc attgcggcgc cactgacgcc gggtcggcca ggtaggcctc gtacagctcc
     8941 tcgatgaacg gcgcgttggc gccgaacagg taggaagtgc cgagatattc cttcagcaga
     9001 gcggccatct gtcggtgact cccgttgtcg gtgacccctg gcttgctggt gcgtgatgcg
     9061 ttcccgctgt gcgcccggct acctgcgcat cgtaagatcg ggcacgtcgc gctcggggga
     9121 cccggtaaag agctggcgcg ggcgtccgat cttctgttcc ggatcggcga tcatctcgtt
     9181 ccactgcgcc acccagccga cggtgcgcgc cagggcgaag atggcggtga acatgctggt
     9241 cggaatgccc atcgcgcgct gc
//
LOCUS       DASPGE010000401         5826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1510699, whole genome shotgun sequence.
ACCESSION   DASPGE010000401 DASPGE010000000
VERSION     DASPGE010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510699"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1550)
                     /locus_tag="VD791_13055"
     CDS             complement(204..1550)
                     /locus_tag="VD791_13055"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HXV08956.1"
                     /translation="MSTTGRDVDAAHLHPEVRARVERVLARLTAERIPFRLFEGFRAP
                     QRQQYLWDQGRVRPGAKVTNAAPWQSMHQYGLAADLVLWENGKWSWDDRGPKGAWWTR
                     LKQVGHEEGLRSISWEAPHLELAGVTLAQLQRGEYPDGGDEGWAAHLVEALYGWTGTP
                     PSPPLPRQVPERPPLPAAAAVPVAAATAALPAPGSEGWHSRFGGREWRYDERGVYLRT
                     HAGGREPLRSPGAPVTMRTIWQSFGGAIATAAQRLGVPPALIMTMIANETGNLRKFGF
                     TGPLSFRWEPHVLVNDVNPPRRGDYSAGPMQVLASTARWIVRAQRLAHDPFAAAPAYE
                     MRPEPPAGHPMYDPALNIELGTAVIRQRWAASGADPILAAAAYNAGGIHRSGENDWRL
                     RCYGDHLDRTARWYGDACSVLQEAGALAPPAPATRRRAAAGKRGRATRQRAARRGR"
     gene            1745..2683
                     /locus_tag="VD791_13060"
     CDS             1745..2683
                     /locus_tag="VD791_13060"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HXV08957.1"
                     /translation="MAQSELPFGVALENVWADYRRCAARSRALKRRLDAIRLWALGLF
                     IGGAALGVLAAQLPLPDAAVEALALVSAAAIALSAYLTAQALDAGLERGWIEARSVAE
                     GLKSEAYRFLAKARPYNAADAPERLAETVRGLRLRLRLGEFAHIEDAERLAGLPKSWL
                     GPDEYLRQRVRDQIDWFERRAAERARKAQRLKLCTIALGALGVVLAAAHGVYPQADAL
                     PAWVGVIGTVSGAITTYLFANRLLFLTASYETTALRLRFALEDWSRVSEEGRAAALGA
                     LVAKVEDILLLENRQWVAEFDRPPEEPATQPSGASS"
     gene            complement(2680..3831)
                     /locus_tag="VD791_13065"
     CDS             complement(2680..3831)
                     /locus_tag="VD791_13065"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXV08958.1"
                     /translation="MSIAERIRRPGAKKILALDGGGILGLMSVEILARIEDELRRRLA
                     PTSASRSGGAGFVLADYFDFVCGTSTGAVIASCIASGMSMARVRAFYLDSGKEMFDPA
                     ALLSRLHYKYEDEPIARKLRTELAEALGEDATLGSPGLRCLLMMVMRNASTDSPWPVS
                     NNPFAKYNDRARADCNLDIPLWQLVRASTAAPTYFPPEVVRVGGKTFVFVDGGVTTYN
                     NPAFLAFLIATAAPYRVGWETGEDKMLIVSIGTGSAPRENAALKPSDLHLLYNAASIP
                     AALMNAAGAGQDLACRVLGKCLYGEPIDREVGDLVQQQVPTSSAPKLFTYVRYDPVVT
                     RAGLDALGLRDVDAAHVQTLDSVAYIPEIQRVGEAYAAQHVRPEHFARF"
     gene            complement(3917..4075)
                     /locus_tag="VD791_13070"
     CDS             complement(3917..4075)
                     /locus_tag="VD791_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08959.1"
                     /translation="MAKPNYAFAKRQRDLAKKQKKEEKRQRKAKPTTEQPPQEPPAPP
                     PGDEGTVS"
     gene            4258..5514
                     /locus_tag="VD791_13075"
     CDS             4258..5514
                     /locus_tag="VD791_13075"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV08960.1"
                     /translation="MSDTVLLQGGKVWDGSGRPAFPADVLVRGNRIARVGEKLARDAG
                     AAARIDVSGQTVIPGLIEGHAHLSFAHKASSADIGDIPPEDHTLLTLLHARIVLEAGF
                     TSCLSGGSAKPRLDVAVRDFIAAGHAPGPRLLAASPELTNTGNLGDERRLHLYRESFA
                     MVVDGVDQMLRTVRMLIREGVDTIKLNISGNQTVASARSHITVMRDDEVAVAAETAHV
                     HGKRVAAHVRSAGSIKLALRHGVDILYHCEYADQECLDLMEAARDRIFVAPAIGLLHN
                     LMHEAQAWGITYQRAKDVGVVDQFEHAQAVFPQLRKRGVRVLIGGDYGFPWTPHGTNA
                     RDLEYFVRFLGYSDSEALVCATRTGAAAMLLQGQTGEVREGLLADLLVIAGEPQNDVR
                     VLQVPANVKLVMKDGRIHRNLFTPAQ"
     gene            complement(5564..>5826)
                     /locus_tag="VD791_13080"
     CDS             complement(5564..>5826)
                     /locus_tag="VD791_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXV08961.1"
                     /translation="ELGMPAIEHMGWSGIFAPARTPREIIARLNTELVRILRSPELIQ
                     IIVDSGGDPDAVGGSPEEVAAFVRREQETLAALIQATGMKVE"
BASE COUNT          838 a         1992 c         2099 g          897 t
ORIGIN      
        1 ggttgctatg ctggcaggcg ggacactgca cggcgccgag acctcagccg gaggcgtagt
       61 cagcgcgaat gctacacgct tcgccatcgg tcaacatcat ggatgccatg acgcgtgcgt
      121 ggccggtgga gatcgacacc cggccactcg tcgagatctg gtgcgccgcg taggtcttga
      181 ggcaggtgct gcccggccgt ccgtcagcgc cctctgcgcg ccgctctctg gcgggttgcg
      241 cgcccgcgct tgcccgctgc cgcgcggcgc cgcgttgcgg gggccggcgg cgccagtgca
      301 ccggcttcct gcagcacgct gcaggcgtcg ccgtaccatc tggcggtgcg gtccaggtgg
      361 tcgccgtagc agcgcaggcg ccagtcgttc tcgccgctgc gatggatgcc tccggcgttg
      421 taggcggcgg ccgccaggat cggatcggcg ccgctcgccg cccagcgctg cctgattacc
      481 gcggtaccca gctcgatgtt gagcgccggg tcgtacatgg gatggccggc cgggggctcg
      541 ggccgcatct cgtaggcggg tgcggcggcg aaggggtcgt gcgcgagacg ctgcgcgcgc
      601 acgatccagc gcgcggtgct cgccagcacc tgcatcggcc cggcgctgta gtcgccgcgc
      661 cgcggcggat tgacgtcgtt caccagcacg tgcggctccc agcggaagga cagcgggccg
      721 gtgaagccga acttgcgcaa gttgccggtc tcgttcgcga tcatcgtcat gatcagcgcc
      781 ggcggcacgc cgaggcgctg cgcggccgtg gcgatggcgc cgccgaagct ctgccagatc
      841 gtgcgcatgg tgaccggcgc gccgggcgag cgcagcggct cgcgcccgcc cgcatgggtg
      901 cgcaggtaca caccgcgctc gtcgtagcgc cactcgcggc cgccgaagcg actgtgccag
      961 ccctcgctgc cgggcgccgg caaggccgcg gtcgcggccg cgacgggtac cgccgcggcc
     1021 gcgggaagcg gcgggcgttc cggaacctgg cgcggcagcg gcggcgaggg cggagtgccg
     1081 gtccagccgt agagcgcttc gaccagatgc gcggcccagc cttcgtcgcc gccgtccgga
     1141 tactcgccgc gctgcaactg cgccagcgtc acgccggcca gctccaggtg cggcgcctcc
     1201 cacgagatcg accgcaggcc ttcctcgtgg ccgacctgct tcaagcgcgt ccaccacgcg
     1261 cccttcggcc cgcgatcgtc ccagctccac ttgccgtttt cccagagcac caggtccgcc
     1321 gccaggccgt actggtgcat cgattgccac ggcgccgcgt tggtcacctt ggcccccggc
     1381 cgcacgcgcc cctggtccca gaggtattgc tggcgctgcg gcgcgcggaa accttcgaac
     1441 aggcggaacg gaatgcgctc ggcggtcagt cgcgccagca cgcgctcgac gcgtgcgcgc
     1501 acctcggggt gcaagtgcgc cgcgtccaca tcgcggccgg tggtgctcaa cggcttgggc
     1561 gcggcaacgg gagcacgcgc ggatgatcgc gtggcacggg gggaggatct gcgcacgctg
     1621 cgctttgctc tcgccatgaa ggggcctcgt ttcgagtagt tgacgacgcg tctagtcgct
     1681 tgcgttcgtg ccgcgatcga ttatggtagc gtggttgccg cggatgcgtt ctcggggagg
     1741 aatcatggcg caatcggagt tgcctttcgg cgtggcgctg gagaacgtct gggccgacta
     1801 tcggcgttgc gctgcgcgct cgcgcgcgct caagcgccgc ctcgacgcca tccgcttgtg
     1861 ggcgcttggg ctgttcatcg gcggcgccgc gctgggcgtg ctcgccgctc agctcccact
     1921 gcccgatgcc gcggtcgaag cgctcgcgct ggtgagcgcc gcggcgatcg ctctgagcgc
     1981 ctacctcacc gcgcaggcgc tcgacgccgg gctcgagcgc ggctggatcg aggcgcgctc
     2041 ggtggccgaa gggctcaagt ccgaggccta ccgctttctc gccaaggctc ggccctacaa
     2101 cgctgcggat gcgcccgagc ggctggccga gacggtgcgg ggcttgcgat tgcggctgcg
     2161 tctaggcgag ttcgcgcaca tcgaggacgc ggaacggctc gcgggcctgc ccaagagctg
     2221 gctcgggccc gacgagtacc tgcggcagcg ggtgcgcgac cagatcgact ggttcgagcg
     2281 gcgtgcagcg gagcgtgcac gcaaggcgca acggctcaaa ctgtgcacca tcgcgctcgg
     2341 cgctctgggc gtggtgctgg cggcggcgca cggcgtgtac ccgcaggcgg acgcgctgcc
     2401 ggcatgggtg ggcgtcatcg gtaccgtgtc gggagcgatc accacttacc tgttcgccaa
     2461 ccgcctgctg ttcctgaccg cgagttacga aaccacggcg ctgcgcctgc gcttcgcgct
     2521 cgaggattgg agccgcgttt ccgaggaagg ccgggcggcc gcgctcggcg ctctcgtcgc
     2581 caaggtcgag gacatcctgc tgctcgagaa caggcaatgg gtggccgagt tcgatcgccc
     2641 gccggaagag ccggcgaccc agccatcggg ggcgagcagc tagaaccgcg cgaagtgctc
     2701 gggccgcacg tgctgcgcgg cgtaagcctc gcccacgcgc tggatctccg ggatgtaggc
     2761 gaccgaatcc agcgtctgca catgcgccgc atcgacgtcg cgcagaccga gcgcgtccag
     2821 gcccgcacgc gtgaccaccg gatcgtagcg cacataggtg aacagcttgg gcgcggaaga
     2881 ggtcggcacc tgctgttgga cgaggtcgcc gacttcgcgg tcgatcggct cgccgtagag
     2941 gcacttgccg agcacgcggc aggcgaggtc ctgacccgcg ccggctgcgt tcatcagggc
     3001 ggcggggatc gacgccgcgt tgtacagcag atgcagatcc gaaggcttga gtgcggcgtt
     3061 ctccctgggc gcgctgccgg tgccgatcga cacgatcagc atcttgtcct cgccggtctc
     3121 ccagcctacc cggtagggcg cggcggtggc gatgaggaag gccaggaacg ccgggttgtt
     3181 gtaggtcgtg acaccgccgt ccacgaacac gaaggtcttg ccgcccacgc gcaccacctc
     3241 gggcgggaaa taggtcggcg ccgccgtgct cgcgcgcacc agctgccaca gcgggatgtc
     3301 gaggttgcag tccgcccgcg cgcgatcgtt gtacttggcg aacgggttgt tggacaccgg
     3361 ccacggcgag tcggtggagg cgttgcgcat caccatcatc agcaggcagc gcaggccggg
     3421 gctgccgagc gtcgcgtctt ctcccagcgc ctcggccagc tcggtgcgca gtttcctggc
     3481 gataggctcg tcctcgtact tgtagtgcag gcggctcagc agggcggcgg ggtcgaacat
     3541 ttccttgccg ctgtcgaggt agaaggcgcg gacccgcgcc atcgacatgc cgctcgcgat
     3601 gcagctcgcg atcacggcgc cggtgctggt gccgcacacg aaatcgaagt agtccgccag
     3661 cacgaagccg gcgcctcccg atctcgacgc cgacgtcggg gcaagccgcc ggcgcagctc
     3721 atcctcgatg cgcgccagga tctccacgct catcaggccg aggatgccgc cgccgtccag
     3781 cgccagaatc ttcttcgcgc ccgggcggcg gatgcgctca gcgatgctca tcgaacctca
     3841 tgggaagggc gcagccgctt cgatgccgag cgccgggctc gccctcaagg gcagcctgca
     3901 cgggctcgcc gcgtgatcag gagacggtac cctcgtcgcc aggcggcggg gcagggggct
     3961 cctgcggtgg ctgctccgtg gtcggcttgg ccttccgctg ccgcttctct tccttcttct
     4021 gcttcttggc gaggtctctc tggcgcttcg cgaacgcgta attgggtttg gccatccggc
     4081 ggctcctgtg gcgggtttcg ggggggaatc caagccgaca gagtaaggca tttccggtgc
     4141 cgccgctttc gggagcgcag gcggtcgaga cgcaggtccc gcaggtgtaa ggtgcgcgag
     4201 cccgcgggcg ccggggtata gtcgccctgc tactgcgtgc gatacgagag ctgcgccatg
     4261 tccgatacgg tattgctgca agggggtaag gtctgggacg gcagcggccg ccccgcgttt
     4321 cccgccgatg tgctggtccg aggcaaccgc atcgcccgag tgggcgagaa gctggcgcgc
     4381 gacgccggtg ccgccgcgcg catcgacgtg tccgggcaaa ccgtcatccc cggcctgatc
     4441 gagggccacg cgcacctgtc cttcgcccac aaggcgagca gcgccgacat cggggacatc
     4501 ccgccggagg accacacact gctgacgctg ctgcacgcgc gcatcgtgct ggaagccggc
     4561 ttcacatcct gcctgagcgg cggttcggcc aagccccggc tcgacgtcgc ggtgcgcgat
     4621 ttcatcgccg ccgggcacgc acccggcccg cggctgctgg ccgccagccc ggagctgacc
     4681 aacaccggca acctgggcga cgagcgccgc ctgcacctgt accgagagtc attcgccatg
     4741 gtggtcgacg gcgtggacca gatgctgcgc accgtgcgca tgctgatccg cgaaggcgtg
     4801 gacaccatca agctcaacat ctccggcaac cagaccgtcg ccagcgcgcg ctcgcacatc
     4861 accgtcatgc gcgacgacga ggtcgccgtg gcggccgaga ctgcgcacgt gcacggcaag
     4921 cgcgtcgccg cccacgtgcg ctcggcgggg tccatcaagc tggcgctgcg gcacggggtg
     4981 gatatcctgt atcactgcga gtacgcggac caggaatgcc tggatctgat ggaagccgcg
     5041 cgcgacagga tcttcgtggc gccggcgatc ggtctgctgc acaacctcat gcacgaagcg
     5101 caggcctggg gtatcaccta tcagcgggcc aaggacgtcg gcgtggtgga ccagttcgag
     5161 catgcgcagg cagtgttccc gcagctgcgc aagcgcggcg tgcgggtgct gatcggcggc
     5221 gactacggct tcccgtggac gccgcacggc accaacgcgc gcgatctcga gtacttcgtg
     5281 cgcttcctgg ggtacagcga cagcgaagcg ctggtgtgcg caacgcgcac cggggcggct
     5341 gccatgctgc tccaagggca gaccggggag gtgagagaag ggctgctcgc cgacctgctg
     5401 gtgatcgcgg gcgagccgca gaacgacgtg cgcgtgctgc aggtcccggc caacgtcaag
     5461 ctggtgatga aggacggccg catccaccgc aacctgttca caccggcgca gtgagagaag
     5521 gccgacacgg ccaaagcgga tgcggtctcg acgcgggacg cggttactcg accttcatcc
     5581 ccgtcgcctg gatcagggcg gcgagcgtct cctgctcgcg ccggacgaag gcggcgacct
     5641 cttcgggaga gccgccgacc gcgtcggggt cgccgcccga atcgacgatg atctggatca
     5701 gctcgggcga gcgcaggatg cgcaccagct cggtgttgag ccgagcgatg atctcgcgcg
     5761 gcgtgcgcgc gggcgcgaaa atgccggacc aacccatgtg ctcgatggca ggcatgccga
     5821 gctcgg
//
LOCUS       DASPGE010000402        10622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_458729, whole genome shotgun sequence.
ACCESSION   DASPGE010000402 DASPGE010000000
VERSION     DASPGE010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10622
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458729"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VD791_13085"
     CDS             <1..839
                     /locus_tag="VD791_13085"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXV08962.1"
                     /translation="LARAIGQKLTQAWGHPVVVENKPGANGNVGADLVAKAPADGHTV
                     LLTDIGGLCISASVYPALPFDPARDFAPVVLISYSPHILGVHPAVPARTVKELVALAK
                     SKPGGLNFATAGIGSAPHLAGVEFALRAGVQWTYVPYKGGSAALADVVAGHADVLFNG
                     MLPTYPFVTGGKLRAIAVSSAKRAAAAPDLPTVAESGLPGFETGSWQGVLAPAGTPRE
                     IVARLNAEVRRILALPDVEERLAAQGTEVRAGSPGALAAFMRAEIARWAKVVKQVGLK
                     IE"
     gene            complement(958..1785)
                     /locus_tag="VD791_13090"
     CDS             complement(958..1785)
                     /locus_tag="VD791_13090"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV08963.1"
                     /translation="MNDYSIREVQRTLGLSRGMILGLVKSGYVTPEVGPRHAYRFTFR
                     DLVVMRTAQELIAARIPPRRVAQTLKRLRAKLPGELPSGGLRIRAADDRIVVQDGARQ
                     WEAGTGQYVLDFSVSGAPGSVRVIAPSAVPAPSAPKAASASGLFDQGCELEEQDARRA
                     ASAYQRAIETDPSFASAYVNLGCLLHEQGRLSEAEAVYRNGLSHCPADALLHYNLGVL
                     LEDLGRRKAALEAYREALALQDDLADCHYNIALLYEASGMTRAALRHLNAYRRLQSG"
     gene            complement(1808..2716)
                     /locus_tag="VD791_13095"
     CDS             complement(1808..2716)
                     /locus_tag="VD791_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HXV08964.1"
                     /translation="MARSIASATISFGMVSIPVDLYPATQSSAGISFNLLHKTCGSRL
                     RQQYVCIKEGVVVERAEMVKGYEFAEDRYVTFTKEELKALEERPTQMIEILEFVGADA
                     IDPIFYDKAYYLAPGKGGAKPYALLRQAMQDTQRFGLGKWAARGKQYIVQVRPVADGI
                     VLQQLLYADEVRRIGEIDIDKAEVRPPELKLATQLIAQISSDKFDPAQYEDEEKKRIE
                     AAIQKKIEGEEIAVSPEEGRPGAQIIDLMDALRASLEKGGKPAPVREAAAERKPPKRA
                     AQNKSVEAAKTAETAKPAARAKAGRK"
     gene            complement(2754..5519)
                     /gene="ligD"
                     /locus_tag="VD791_13100"
     CDS             complement(2754..5519)
                     /gene="ligD"
                     /locus_tag="VD791_13100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HXV08965.1"
                     /translation="MTRNDRMAKGQAREAEGGDAVAGLKDYSTKRSFEATPEPPPKPA
                     VARRGPLLFVVQQHSARRLHFDFRLELDGVLKSWAVPDGPSLDPKVKRLAVPTEDHPF
                     DYASFEGVIPPKQYGAGEVIVWDCGVYSPDADGAYAFQDREEAQRRVRAEHARDKLSF
                     FLLGEKLKGSWTLVKMKDKDWLLIKHKDRFARNDVDIRARARSVLSGYAVEDLKAPKP
                     LARLDAQQLIPNGPPEAPPNKLAPMLAAAADKPFNDPAFLYEPKLDGYRCIAIRRDGK
                     VRLLSRRGLDQSAQFPEILGALQEQVLDPMVLDGEILAHDQTGRPSFNALQNRAQLKT
                     EREVAAARAGAPCVLYVFDLLHVCGINLRNAEYAARKRYLGQCVLPSEHVKILGATDT
                     DGVRFYRAALEAGFEGAVAKRRNSRYEAGVRSPAWLKLKATQSGDFLVCGYTVGKGTR
                     AKTFGALILGYRDQDGKLTCAGRVGSGFDERKLAELAARFKGLERKKAPFTELPVLDA
                     PAVWLEPELVVEVEFAEWTPDGSLRAPVFVRLREDIPKEDAGAPAVVHIAKAASAGAL
                     AQPDQIAQVIDQLRGKQDEMVLQVGADRIRLSSLNKPFWPKNQKLKQPLVTKRDLLVY
                     LARVSPYLLPHVVDRPLTMIRFPEGIGGHRFFQKHWEHTLPEFIETITVYSESKQENG
                     EYLLCNNLPTLLWLGQMGTLEYHVWHSRASLYPDAQESSTHFTDSEENIESSILNFPD
                     YVVFDLDPYIYSGKEAPGEEPELNTTAFEKGKEVAFWLRELLEGMKLHPVVKTSGKTG
                     LHIFVPIVRNLTFTAVREICETLGRYLMQRHADAITMEWSIPKRTGKIFLDSNMNVRS
                     KTLNCAYSPRGVAGAPVSMPLTWDELAKAHPMDFRMWNVFERLERQGDTWRDVIALKT
                     DLRNTLG"
     gene            complement(5568..6281)
                     /locus_tag="VD791_13105"
     CDS             complement(5568..6281)
                     /locus_tag="VD791_13105"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXV08966.1"
                     /translation="MLLLAGTSGFSYSEWKGNFYPAKLPAAGMLRYYAARFPTVEINN
                     TFYRMPAESMLSRWAREVPAGFSFTLKAPQRITHHKRLREAEQEVSMFLQRAAALGDK
                     LGPVLFQLPPVLRKDVPRLEDFLALLPGDGNRFAFEFRHASWQDDAVYAVLRARGAMT
                     CVADTDKGDTPLMATADQGYLRLRRTQYDDGELRAWAERVAAQPWGRAYVYFKHEDEG
                     LGPQFAQRFIEAWAQVHGK"
     gene            complement(6325..6771)
                     /locus_tag="VD791_13110"
     CDS             complement(6325..6771)
                     /locus_tag="VD791_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HXV08967.1"
                     /translation="MRPIEALTSLLLLACMSAPALAAQPPSERGRIDYVLHCSGCHAL
                     DGSGVETKGIPQVKDRIGYFLRFPEGRAFLLQVPGLLAAGLSDERAAGVTNWMIEYFA
                     GPSLPPEFIPYTAAEARRYRLSKPADIPGVRQALETKLREVGYPLP"
     gene            complement(6861..7379)
                     /locus_tag="VD791_13115"
     CDS             complement(6861..7379)
                     /locus_tag="VD791_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase light chain"
                     /protein_id="HXV08968.1"
                     /translation="MKSVQEWIDRFAECRVRQAAQLSGRRSALAKLGSVMVGSAFALP
                     MLPFDRSGKAHAAGPAGLDDQACDYWRNCALDGFLCTCCGGTVSACPPGSDVSKVTWV
                     GTCANPKDGRDYLVSYNDCCGKTACGRCLCNYNDRERPGYRMGVHNDINWCMANTQTM
                     YHCTISAIVGTA"
     gene            complement(7416..8027)
                     /gene="mauD"
                     /locus_tag="VD791_13120"
     CDS             complement(7416..8027)
                     /gene="mauD"
                     /locus_tag="VD791_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase accessory protein
                     MauD"
                     /protein_id="HXV08969.1"
                     /translation="MSSLLISNALLWLLMLGVIVALWALARQIGVLYERIAPMGALVT
                     DSGPKLGEAAPKFELPALSGARVAIGGERPKSLLVFFLSPTCPVCKKLLPILKSASQA
                     EREWLDVVLASDGEATQHLAFYGDAKLKDFAYVLSADLGMTYRVSRLPYAVLIDERGM
                     IRAKGMINTREHLESLFNAKELGVGSVQDYLRERLPAQSGETV"
     gene            complement(8024..8596)
                     /locus_tag="VD791_13125"
     CDS             complement(8024..8596)
                     /locus_tag="VD791_13125"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HXV08970.1"
                     /translation="MQAAALTADPVAVGAVVGALALVLFAAAWHKLAQGEVFAAALQA
                     YELVPVSAVLAVARALPVVELAIGVGVLVPVTRQPALIAFAALMLLYAGAMGVNLIRG
                     RRQIDCGCGGEAHPLSWGLVLRNAVLAALALAVSGPTSQRGFEWLDGVTLLAGVLAFY
                     GLYLLFDELLRQGSRLGRLRAYDREEVAAK"
     gene            complement(8596..9744)
                     /locus_tag="VD791_13130"
     CDS             complement(8596..9744)
                     /locus_tag="VD791_13130"
                     /inference="COORDINATES: protein motif:HMM:NF018172.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amine dehydrogenase large subunit"
                     /protein_id="HXV08971.1"
                     /translation="MGSSNRWAAAGGAVAMLAVTLCASTAFAQLATEEVITLPAITAK
                     NRVYVLDIAINHIADGKLHVVDADKMAYLGVIGTGFTGQIIQSPDGKELIVATGYLSR
                     GQRGERTDIVEVWDADTLTFKYEIPIPDKRAMALNYEGLVRLSADGRWLFVQNATPAT
                     SVTVVDMQARETAGEISMPGCWAIYPAASNPNRFASLCGDGSIATVTLDDSGKVASRS
                     ATQKVFDPDEDAWFMSGEQEGDLYHFVSFKGRLITVDVSGDTATVEAPWPLVPAADRK
                     RGWRPGGYQPIAVHGGRLYVAMHPGGAEGSHKNPAAEVWAYDIAKRKRLARLPGRNAI
                     ALETSSDGSKLYALDALKAEVVVYERIGPKPRIRSMQVGETSVQLVAN"
     gene            complement(9826..>10622)
                     /gene="egtB"
                     /locus_tag="VD791_13135"
     CDS             complement(9826..>10622)
                     /gene="egtB"
                     /locus_tag="VD791_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HXV08972.1"
                     /translation="SRNPLRPVYRVGAASAPPAVSHSGAPLSWIELPSGIREIGHDGA
                     GFSFDNELPRHRVFVDGFRLASRLTTNAEYLAFMQDGGYQRPQLWLSDGWDACSALRW
                     NAPLYWEREDGRWRAFTLRGMVDVDPDEPVCHVSYYEADAYARWAGARLPTEAEWEVA
                     AAAAPVRGNLVESQRFHPALAGTGPGLQQAFGDVWEWTGSAYLPYSGYRPAAGAVGEY
                     NGKFMVNQFVLRGGSCATPQSHIRASYRNFFPPAARWQFSGIRLAR"
BASE COUNT         1648 a         3726 c         3515 g         1733 t
ORIGIN      
        1 tcctggcgcg cgcaatcgga cagaagctga cgcaggcctg gggccacccg gtcgtggtcg
       61 agaacaagcc gggggccaac ggcaacgtcg gcgccgatct ggtcgcgaag gcgccggccg
      121 acggccatac cgttctgctc accgacatcg gcggcctgtg catcagcgcc agcgtgtatc
      181 cggcgctgcc gttcgatccg gccagggact tcgcaccggt ggtgctgatc agctactcgc
      241 cgcacatcct cggcgtgcat cccgcggtgc cggcgaggac ggtgaaagag ctggtggccc
      301 tggcgaagtc gaagcccggc ggcttgaact tcgccaccgc gggaatcgga agcgcgccgc
      361 atctcgccgg cgtcgagttc gcgttgcgcg ccggcgtgca atggacgtac gtgccgtaca
      421 agggcgggtc ggcggcgctg gccgacgtgg ttgccgggca tgccgacgtg ctgttcaacg
      481 gcatgctgcc cacgtatcca ttcgtgaccg gcggcaagct gcgggccatc gccgtttcct
      541 ccgccaagcg cgccgccgcc gcgccggatc tgcccaccgt ggccgaatcc ggcctgcccg
      601 ggttcgagac cggctcctgg cagggcgtgc tggctcccgc cggcacgccc cgcgagatcg
      661 tcgcccggct caacgccgag gtgcggcgca tcctcgcgct gccagacgtc gaggagcggc
      721 tcgccgcgca gggcacggag gtgcgcgcag gcagccccgg tgcgcttgcc gctttcatgc
      781 gcgcagagat cgcacgctgg gcgaaagtgg tgaagcaggt cgggctgaag atcgagtagc
      841 ttgggggaaa ctcccctcct cgtcgaggag gggggcacac gaagcgtgac ggggtggtcc
      901 agcagccgca caccacaccg ctccgcgggc agcccgccgc gtgagaggcg gggaacgcta
      961 gcccgactgc agcctgcgat acgcgttcaa gtggcgcagg gccgcccggg tcatccccga
     1021 ggcctcgtag agcagcgcga tgttgtagtg gcagtcggcc agatcgtctt gcaacgccag
     1081 cgcttcacgg taggcttcga gcgccgcctt cctgcgcccg agatcttcca gcagcacgcc
     1141 caggttgtag tgcagcaacg cgtccgccgg gcagtgcgag agaccgttgc gatacaccgc
     1201 ttcggcttcg ctcaagcgcc cctgctcgtg cagcaggcac cccaggttga cgtaggcgct
     1261 ggcgaaggac gggtcggttt cgatcgcgcg ctggtaggcc gaggcggcgc ggcgtgcgtc
     1321 ctgctcttcc agctcacagc cctgatcgaa caaacctgag gccgaggccg ccttgggagc
     1381 agatggcgca ggcacagccg atggggcgat cacgcgtacg ctcccggggg cgccgctcac
     1441 cgagaagtcc agcacatact gaccggtgcc ggcctcccat tgtctggcgc catcctgcac
     1501 cacgatgcga tcgtccgccg cccggatgcg caagccgccc gacggcagct cgcccggcag
     1561 tttcgcgcgc agccgtttca gggtttgcgc gacgcggcgc ggcggaatgc gcgccgcgat
     1621 cagctcctgc gcggtgcgca tcaccaccag atcgcggaac gtgaaccggt aggcgtgacg
     1681 cggcccgacc tcgggagtga cgtacccgga cttcaccagc cccaggatca tgccgcggga
     1741 caacccgagg gtgcgctgca cctcgcggat cgagtagtcg ttcaccgtcg ctcgggaatc
     1801 ggctccgcta cttcctgccg gccttggccc ttgcggcggg cttggcggtc tcggccgttt
     1861 tcgccgcttc gaccgacttg ttctgggcgg cgcgcttcgg cggcttgcgc tccgcggcgg
     1921 cctcccgcac cggcgccggc tttccgccct tctccaggct ggcgcgcaac gcatccatca
     1981 ggtcgatgat ctgggcgccg ggccggccct cctcgggcga gacggcgatc tcttcgccct
     2041 cgattttctt ctggatcgcg gcttcgatgc gtttcttctc ctcgtcctcg tactgcgcgg
     2101 gatcgaactt gtcggatgag atctgcgcga tcagctgtgt ggcgagcttc agctccggcg
     2161 ggcgcacctc ggccttgtcg atgtcgatct cgccgatcct gcgcacctcg tcggcgtaca
     2221 acagctgctg cagcacgatg ccgtccgcca ccgggcgcac ctgcacgatg tactgcttgc
     2281 cgcgcgcggc ccacttgccg aggccgaagc gctgcgtgtc ctgcatcgcc tgccgcagca
     2341 gcgcataggg cttggcgccg cccttgccgg gcgccagata gtaggccttg tcgtagaaga
     2401 tcggatcgat cgcgtcggcg ccgacgaact ccaggatctc gatcatctgc gtgggccgct
     2461 cctcgagggc cttcagctcc tccttggtga aggtgacgta gcggtcctcc gcgaactcgt
     2521 agcctttcac catctcggcg cgctcgacga ccacaccttc cttgatgcag acgtactgct
     2581 gcctgagccg cgagccgcag gtcttgtgca acaggttgaa cgagatgccg gcgctgctct
     2641 gcgtggcagg gtacaggtcc accgggatcg acaccatgcc gaacgagatc gttgccgatg
     2701 cgattgagcg tgccatgatg ctcctttctg cccgcctgct gcgcggggaa actttagccc
     2761 aaagtgttgc gcaagtccgt cttgagcgcg atgacgtcgc gccaggtgtc gccctggcgc
     2821 tcgaggcgtt cgaacacgtt ccacatcctg aagtccatgg gatgggcctt cgccagctcg
     2881 tcccaggtaa gcggcatcga caccggggcg ccggccacgc cgcgcggcga gtaggcgcag
     2941 ttcagcgtct tgctgcgcac attcatgttg ctgtccagga aaatcttgcc ggtgcgcttg
     3001 gggatgctcc actccatggt aatcgcatcc gcgtgccgct gcatcaggta acgtcctaga
     3061 gtttcgcaga tttcccgtac cgccgtaaag gtcaagttgc gcacgatggg cacgaagatg
     3121 tgcaggccgg tcttgcccga agtcttgacc acggggtgca gcttcatgcc ttcgagcagc
     3181 tcgcgcagcc agaacgccac ctccttgccc ttctcgaaag cggtcgtgtt gagctccggc
     3241 tcctctccgg gggcctcctt gcccgagtag atgtacggat cgaggtcgaa cacgacgtag
     3301 tccggaaagt tcaggatcga ggactcgatg ttctcctcgc tatccgtgaa gtgcgtgctg
     3361 gattcctgcg cgtccggata gaggctggcg cgcgaatgcc acacgtgata ctcgagggtg
     3421 cccatctggc cgagccagag cagggtgggc aggttgttgc acagcaggta ctcgccgttc
     3481 tcctgcttgc tttcggaata caccgtgatc gtctcgatga actccggcag cgtgtgctcc
     3541 cagtgcttct ggaagaagcg gtgcccgccg atgccctccg ggaaacggat catggtcagg
     3601 ggccgatcca ccacgtgcgg gagcagatag ggcgagacgc gcgcaaggta aacgagcagg
     3661 tcgcgcttgg tcaccagcgg ctgcttgagc ttctggttct tcggccagaa cggcttgttc
     3721 aggctgctca gtcggatccg atccgccccg acctgcagca ccatctcgtc ctgcttgccg
     3781 cgcaactggt cgatgacctg cgcgatctga tccggctgcg ccagtgcacc ggcggatgcc
     3841 gctttcgcga tgtgtaccac cgccggtgcc ccggcgtctt ccttcgggat gtcctcgcgc
     3901 aagcgcacga acaccggcgc gcgcagcgat ccgtccggcg tccattccgc aaactccacc
     3961 tcgaccacca actcgggctc cagccacacc gccggcgcgt cgagcaccgg caattcggtg
     4021 aagggcgcct tcttgcgctc caggcccttg aaccgggccg cgagctcggc gagcttgcgc
     4081 tcgtcgaagc cgctgcccac gcgccccgca caggtgagct taccgtcctg gtcgcgatag
     4141 ccgaggatca gggcgccgaa cgtcttcgcg cgcgtgcctt ttccgacggt gtagccgcac
     4201 accaggaaat cgccgctctg cgtggccttg agcttgagcc atgccggtga gcgcacgccg
     4261 gcttcgtaac ggctgttgcg ccgcttcgcc accgcgccct cgaaccccgc ttccagcgcg
     4321 gcacggtaga agcgcacgcc gtccgtgtcg gtcgcgccga gtatcttcac gtgctccgag
     4381 ggcagcacgc attgccccag ataacgcttg cgcgcagcgt actcggcgtt gcgcaagttg
     4441 attccgcata cgtgcagcag atcgaagacg tacagaacgc acggtgcgcc ggcgcgggcg
     4501 gcggcgactt cgcgttccgt cttgagttgt gcacggttct gcagcgcgtt gaaggacggg
     4561 cggccggtct ggtcgtgcgc gaggatctcg ccgtccagca ccatcggatc gagcacctgc
     4621 tcctgcagcg cgccgaggat ctcgggaaac tgcgcgctct ggtcgagccc gcgccgcgac
     4681 agcaggcgca ccttgccgtc gcgccggatg gcgatgcagc ggtagccgtc cagtttgggc
     4741 tcgtagagga atgcgggatc gttgaacggc ttgtccgcgg ccgcggccag catcggcgcg
     4801 agcttgttcg gcggcgcttc gggcgggccg ttcggaatca actgctgcgc atcgaggcgc
     4861 gcgagaggtt tgggagcctt caggtcctcg accgcatagc ccgacagcac ggagcgggcg
     4921 cgggcgcgga tatccacgtc gttgcgcgcg aaacgatcct tgtgcttgat gaggagccag
     4981 tccttgtcct tcatcttcac cagcgtccac gagcccttga gcttttctcc gagcaggaag
     5041 aagctcagct tgtcccttgc gtgctccgcg cgcacgcgcc gctgcgcctc ctcccgatcc
     5101 tggaaggcgt aggcgccgtc ggcgtccggc gagtagacgc cgcagtccca gacgatgacc
     5161 tcgcctgcgc cgtactgctt gggcgggatc acgccctcga acgacgcgta gtcgaacggg
     5221 tgatcctcgg taggcaccgc caggcgcttg accttcgggt cgagcgaggg tccatcgggg
     5281 accgcccagg atttcagcac gccgtcgagc tccagccgga aatcgaagtg caggcgccgg
     5341 gccgaatgct gctgcacgac gaacagcagc gggccgcgcc gcgccacggc cggcttgggc
     5401 ggcggctcgg gagtcgcctc gaacgaacgc ttggtgctgt agtccttcag cccggcgacc
     5461 gcatcgccgc cctcggcctc ccgggcctgc cccttggcca tcctgtcgtt cctggtcacg
     5521 atcgtggaga ttgtatcggt cgccgggagc gggaaaacag ggcgaggtta cttcccgtgc
     5581 acctgcgccc acgcctcgat gaaccgctga gcaaactgcg gtcccaggcc ctcgtcttca
     5641 tgcttgaagt aaacgtaggc gcggccccag ggctgcgccg ccacccgctc ggcccatgcg
     5701 cgcagctcgc cgtcgtcgta ctgcgtgcgg cgcaggcgca ggtacccctg atctgcggtc
     5761 gccatcagcg gcgtatcgcc tttgtccgtg tccgcgacac acgtcatcgc gccacgcgca
     5821 cgcagcaccg cgtacaccgc gtcgtcctgc cacgacgcgt gccggaactc gaacgcgaag
     5881 cgatttccgt cgcccggcag cagggcaaga aagtcctcca gccggggaac gtccttgcgc
     5941 agcaccgggg gcaactggaa cagcaccgga ccgagcttgt cgccgagcgc cgcggcgcgc
     6001 tgcaggaaca tgctcacctc ctgctcggcc tcgcgcaagc gcttgtggtg cgtgatgcgc
     6061 tgcggcgcct tgagcgtgaa actgaagccg gccggaacct ctcgcgccca gcgcgacagc
     6121 atgctctcgg cgggcatgcg atagaaagtg ttgttgatct ccacggtggg aaagcgcgcg
     6181 gcgtagtagc gcagcatccc cgccgcgggc agcttcgccg ggtagaagtt acctttccac
     6241 tcggaatagg agaagccgct cgttccggcc agcaacagca tcgcggacag gcgctggttt
     6301 cccggccctg gggcttcccg gccgctaggg cagcgggtac cccacctcgc gcagcttcgt
     6361 ctccagcgcc tggcggacgc cggggatatc ggccggcttg ctcaagcggt agcgccgcgc
     6421 ttccgccgcc gtgtagggaa tgaactccgg cggcagggag gggccggcga agtactcgat
     6481 catccagttg gtgacgccgg cggcacgctc gtcggacaga cccgcggcca gcagtccggg
     6541 cacctgcagc aggaacgcgc ggccctcggg aaaccgcagg aagtatccga tccggtcctt
     6601 gacctgcggg atgcccttgg tctcgacgcc gctgccgtcg agcgcgtgac agcccgagca
     6661 gtgcagcacg tagtcgatgc gcccgcgctc ggacggcggc tgcgccgcaa gcgccggcgc
     6721 gctcatgcac gcgagcagca ggagcgaggt caacgcttca atcggacgca tgttcgatac
     6781 cacatggtgc ttcgcgcacc accccgtcac gcttcgcgtg ccacccctcc ttgaaaagga
     6841 ggggaacctg ccgcacaccg ctacgccgtc ccgacgatcg ccgagatggt gcagtggtac
     6901 atggtctggg tgttggccat gcaccagttg atgtcgttgt gcacacccat gcgatagccg
     6961 ggccgctccc ggtcgttgta gttgcacagg cagcggccgc acgcggtctt gccgcagcag
     7021 tcgttgtagc tcaccaggta gtcgcgcccg tccttggggt tggcgcaggt gcccacccag
     7081 gtgaccttgg acacgtccga cccaggcggg caggcgctga cggtgccgcc gcagcaggtg
     7141 cacaggaagc cgtcgagcgc gcagttgcgc cagtaatcgc aggcctggtc gtcgagcccg
     7201 gcgggcccgg cggcgtgggc cttgcccgag cggtcgaagg gcagcatcgg cagcgcgaaa
     7261 gcgctgccca ccatcaccga ccccagcttg gcgagcgcgc tgcgccggcc ggagagctgc
     7321 gccgcctgcc gcacgcggca ctcggcgaaa cggtcgatcc actcttgaac gctcttcatc
     7381 ttctcccccg ttgcatggtc acccagtggg tgaggtcaaa cagtctcgcc ggactgcgcc
     7441 ggcagccgct cgcgcaggta gtcctgcacc gagccgaccc ccagctcctt ggcgttgaac
     7501 aggctctcca ggtgctcgcg cgtgttgatc atgcccttgg cgcggatcat accgcgttcg
     7561 tcgatcagca cggcgtaagg caggcggctc acccgatagg tcatgcccag gtcggccgac
     7621 agcacgtagg cgaaatcctt gagcttggca tccccgtaga aggccaggtg ctgcgtggcc
     7681 tcgccgtcgc tcgccagcac cacgtccagc cactcgcgct cggcctggga ggcggacttc
     7741 aggatcggca gcagcttctt gcacaccggg caggtgggcg acaggaagaa caccagcagg
     7801 ctcttcggcc gctcgccgcc gatcgccacg cgcgcgccgg acagcgcggg cagctcgaac
     7861 ttcggcgccg cctcgcccag cttggggccg gagtcggtga ccagcgcgcc catcggggcg
     7921 atgcgctcgt acagcacgcc gatctggcgc gccagcgccc acagcgcgac gatcacgccg
     7981 agcatcaaca gccacagcag cgcattggag atcaacaggc tgctcacttg gctgccacct
     8041 cctcacggtc gtacgcgcgc agccggccga gccggctgcc ctggcgcagc agctcgtcga
     8101 acagcagata caggccgtag aaggcgagca cgcccgccag cagcgtaact ccatcgagcc
     8161 attcgaaccc gcgctggctg gtcggccccg agaccgccag cgcgagcgcc gccagcaccg
     8221 cattgcgcag caccagtccc caggacaggg gatgcgcctc gccgccgcag ccgcagtcga
     8281 tctggcgccg tccgcggatc aggttcacgc ccatcgcgcc ggcgtacagc agcatcaacg
     8341 ccgcgaacgc gatcaacgcg ggctggcgcg tgaccggcac cagcacgccc acgccgatcg
     8401 ccaactcgac caccggcaag gcgcgcgcca cggcgagcac ggcgctcacc ggaaccagtt
     8461 cgtaggcctg cagcgccgca gcgaacacct ccccctgcgc gagcttgtgc caggccgcgg
     8521 cgaacagcac cagagccagc gccccgacca cggcgcccac cgcgaccgga tcggcggtca
     8581 gcgcggccgc ttgcatcagt tcgccaccag ctgcaccgag gtctcgccca cctgcatcga
     8641 gcgaatgcgc ggcttgggtc cgatgcgctc gtacaccacg acctcggcct tcagcgcatc
     8701 cagcgcgtac agcttcgagc cgtcggacga ggtctccagg gcgatcgcgt tgcgccctgg
     8761 gaggcgcgcc agccgcttcc tcttggcgat gtcgtaggcc cacacttcgg cggccgggtt
     8821 cttgtggctg ccctcggcgc cgcccggatg catcgcgacg tacagccggc cgccatgcac
     8881 ggcgatcggc tgatagccgc cgggacgcca gcctctcttg cgatcggccg cgggcaccag
     8941 cggccagggc gcttcgaccg tggccgtgtc gcccgacacg tcgaccgtga tcagccggcc
     9001 cttgaagctc acgaagtggt agaggtcgcc ctcctgctcg ccggacatga accaggcgtc
     9061 ctcgtcggga tcgaacacct tctgcgtcgc gctgcgcgag gcgaccttgc cgctgtcgtc
     9121 cagcgtcacc gtggcgatgc tgccgtcgcc gcacagcgaa gcgaaacggt tcgggttgga
     9181 tgccgccgga tagatggccc agcagcccgg catggagatc tcgcccgcgg tttcccgcgc
     9241 ctgcatgtcc accaccgtca ccgaggtggc cggcgtggcg ttctgcacga acagccagcg
     9301 gccgtcggcg gacaaccgca cgaggccttc gtagttgagc gccatggcgc gcttgtcagg
     9361 gatcgggatt tcgtacttga aggtcagcgt gtcggcgtcc cacacctcga cgatatcggt
     9421 gcgctccccg cgctgcccgc gcgacaggta gccggtggcc acgatcagct ccttgccgtc
     9481 tggcgattgg atgatctgtc cggtgaatcc ggtaccgatc acccccaaat acgccatctt
     9541 gtccgcatcc accacgtgca gcttgccgtc tgcgatgtgg ttgatggcga tatccagaac
     9601 gtagacccgg ttcttggccg tgatcgccgg cagcgtgatc acttcctccg tcgccagttg
     9661 cgcgaaggcg gtgctcgcgc agagcgttac ggcgagcatg gcgactgcgc cgcccgcggc
     9721 ggcccagcgg ttagacgatc ccattgcgaa tcctcccggt tgtgcagcgg gctccccgat
     9781 gccccgcggc acagtatagc gccgggggca ggagcacgcc gtcgctcatc gcgccagccg
     9841 gatgccgctg aactgccagc gcgcggcggg cgggaagaaa ttgcggtagc tcgcgcggat
     9901 gtgcgactgc ggtgtcgcgc acgacccccc gcgcagcacg aactggttca ccatgaactt
     9961 gccgttgtac tcgcccaccg cgccggccgc cggccggtag cccgaatagg ggaggtaggc
    10021 gctgccggtc cactcccaga cgtcgccgaa cgcctgctgc aggcccgggc cggtgcccgc
    10081 gagcgccgga tggaagcgct gcgactcgac caggttgccg cgcaccggtg ctgctgcagc
    10141 ggcgacctcc cactccgcct ccgtgggcag gcgtgcgccc gcccagcgcg cgtaggcgtc
    10201 cgcctcgtaa tagctcacgt ggcacacggg ctcgtcggga tccacgtcca ccatgccgcg
    10261 caaggtgaac gcgcgccagc gtccatcctc gcgctcccag tacagcggcg cgttccagcg
    10321 cagcgcgctg cacgcgtccc agccgtcgga cagccacagc tgcgggcgct gataaccgcc
    10381 gtcctgcatg aacgcgaggt actccgcgtt ggtcgtgagc cgcgacgcga gccggaagcc
    10441 gtccacgaag acgcggtggc ggggcagctc gttgtcgaag gagaacccgg cgccgtcgtg
    10501 gccgatctcg cggatccccg acggcagctc gatccagctc agcggcgccc ccgaatgcga
    10561 tactgccggc ggtgcgctcg ccgcgcccac gcgatagacc gggcgcagcg ggttgcgcga
    10621 ca
//
LOCUS       DASPGE010000403        14610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_343616, whole genome shotgun sequence.
ACCESSION   DASPGE010000403 DASPGE010000000
VERSION     DASPGE010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14610
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343616"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VD791_13140"
     CDS             complement(<1..219)
                     /locus_tag="VD791_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV08973.1"
                     /translation="MPGRSAIENLEKLLAAGQDGALLRFGLGSEYLKAGDAGMAAGHL
                     QRALALDPGYSAAWKLLGQALTELSRFSE"
     gene            complement(231..2141)
                     /locus_tag="VD791_13145"
     CDS             complement(231..2141)
                     /locus_tag="VD791_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HXV08974.1"
                     /translation="MEQQQQADGDQPVSHTRRGVLGLAIGAVGVVYGDIGTSPLYAIK
                     ESFGGTHNLPVVHDNVVGVLSVMAWALFSIVTLKYVLLMMRADNRGEGGIMALMALVR
                     RSLDGEPRLRWLLIVLGMFGAALFYGDGMITPAISVLSAVEGLEVITPVLKPYVIPVT
                     LAVITGLFVVQQTGTGRVGAFFGPVMTLWFAVLALLGLISIWHYPSVLEALNPYWGVR
                     FFVSNGTLGFLVLGAVVLAITGAEALYADMGHFGKRPIRLAWLWFVFPALLINYFGQG
                     ALLLREPQAVQHPFFHLVPAWGLLPMVVLATLATVIASQAVITGAYSLTRQAIQLGYC
                     PRLMVEHTSEREIGQVYLPWINWGLYLAVIGLVLGFGSSSNLAGAYGIAVTATMAIDS
                     ILIFFVMRKLWHWPLVLAAPISALFLAVDLSFFSANSLKILEGGWFPVVVGVVVFVLL
                     STWKRGREILFERLRPGAIALQPFIKSITEHPPVRVPGMAVFLTASQEGVPHALLHNL
                     NHNKVLHERVVLLTVRSEDIPHVPDAQRVNLESLGDDFYRLTVHYGFKDEPDLPAALA
                     LAAAQHGMEFNMLETSFFLSRQTLVPTPSPGMALWREKLFAAMSRNAASATVFFKIPT
                     NRVVELGTRIEL"
     gene            2272..2652
                     /locus_tag="VD791_13150"
     CDS             2272..2652
                     /locus_tag="VD791_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HXV08975.1"
                     /translation="MAKQAIHTDEAPQPIGIYSQAVRAGNTLYLSGQIGLDPRTMLLV
                     SGIDAQIHQVFLNLRAVAAAAGASLDDAVKFTVFLTDMTDFPRVNEIMAGYVSQPYAA
                     RSAVAAAGLPRGALVEIEAVLVVG"
     gene            2663..4732
                     /gene="recG"
                     /locus_tag="VD791_13155"
     CDS             2663..4732
                     /gene="recG"
                     /locus_tag="VD791_13155"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HXV08976.1"
                     /translation="MSPQRRAGGEPGISVPDAARKQLDKLGLSSALDLALHLPLRYED
                     ETRLARLRDVRAGDTAQVEVEVLAAEIQFRPKRILVCRARDGDEELTLRFFHFYPSQL
                     RQLAAGVRLRVMGELRRGFFGAEMIHPRWRVVRGGEPLPESLTPIYPSTAGLAQPLLR
                     KLIGRALAAADLSDPLPDALRTHLRLAPFEDSIRYLHNPPPDAPQAALQERTHPAWRR
                     VKFDELLAQQLSMRLHHRRRRSARAPALDPPGPLSARLLAQLPFRLTRAQKRVLAEIR
                     ADLAQPHPMQRLLQGDVGSGKTIVAALAALQAVESARQVAVMAPTEILAEQHHAKLGA
                     WLAPLGVEVAWLAGSLTKKDKRAALARVESGAAQVAVGTHALFQDQVAFRDLALAVVD
                     EQHRFGVHERLALRLKGTLESGELQPHQLMMSATPIPRTLAMSYYADLDVSVIDELPP
                     GRTPVVTKLLSESRREEVTARVRDACMAGRQAYWVCPLIEESEALQLQTAIDTFEGLR
                     RTFPELSVGLVHGRLSSADKTAVMEAFEAGRVQLLVATSVIEVGVDVPNASLMVIEHA
                     ERMGLSQMHQLRGRVGRGAQASVCILLYQEPLSDTARERLRIMYKVSDGFEIARHDLR
                     IRGPGELLGARQSGVPMLRFADLEADLDLLDAARGAADGLLQDAPERARAHLQRWMGG
                     RQEYLRV"
     gene            4821..6842
                     /gene="hyfB"
                     /locus_tag="VD791_13160"
     CDS             4821..6842
                     /gene="hyfB"
                     /locus_tag="VD791_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HXV08977.1"
                     /translation="MAIVPLLHVVLSVVAYWIALGLIGLAVPLERGAHARLFFPLSAL
                     GAGVLAAAGFVGLGAWPEALVLPLGLPDLPFHLRLDPLSAFFLLLLGTAAAGVSLFSA
                     GYFERGGSGDIGLLCLQYHVFLASMVMVLLADDAYLFMVAWESMALASYFLVTTDHRV
                     AEIRSAGLLYLVVAHLGAIAILLAFGVLQLGQWSYTFAAMRDNALTPFWAAVAFLLAL
                     IGFGAKAGFLPLHVWLPEAHPAAPSPVSALMSGVMLKTAVYGLLRVSFDLLQGYTWWW
                     GVAALSVGLVTAFYGVAFAAVQTDMKRLLAYSSIENIGIVLAGLGLALTFHAYNMELL
                     AALALAASLYHCLNHALFKSLLFLATGSVMHATRQRSLGKLGGLIRRMPWVAGLALVG
                     TLAIAGLPPLNGFVSEWMLFQAFLLTPGLPNSYLNMFVPVAAAALALTVALAGYVMVK
                     FYGIVFLGLPREESLEDAHDAGPYERAGLIWLALWCVVLGVTPVYVVQLMDPLTHGLV
                     GGALGESVRRSGWLFLTPVAPERASYAPLLFLLVIVGVVLLTFLVVRRLYHGRVRRAP
                     AWDCGFAGLNARMQDSAEGYGQPIKQIFEPFFRLERHHPSPFDAQPRYFSRIDDRLWY
                     WLYLPVVRLAERVSSLFAVVQHGRIALYLIYSFATLLLLLLLIRWIGAA"
     gene            6839..7786
                     /locus_tag="VD791_13165"
     CDS             6839..7786
                     /locus_tag="VD791_13165"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HXV08978.1"
                     /translation="MNWTDLLSQLFQTLLAALLAPLALGWVNQCRAWLQNRSAPDLLQ
                     PYRVLFKLLEKDAVLAVNASWLFRLAPYVVFACMLLAASIVPLVATDLPFAQAADVIA
                     LVGLFALARVFMALAAMDVGTAFGTLGSRREMLIASLAEPALLMVFFTPALIGQSTAL
                     ATIVEQLAQRELQLNPSMAFAAVAFVMVLLAENARVPVDNPATHLELTMVHEAMILEY
                     SARHLALMEWAHALKLTVYLAIGIALFLPWGIARAGDWGMMPAAILWFAAKLALLGAG
                     LALLETVSAKMRIFRVPEFLGMAFMLAVLGMLIHFLLEA"
     gene            7838..8503
                     /locus_tag="VD791_13170"
     CDS             7838..8503
                     /locus_tag="VD791_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HXV08979.1"
                     /translation="MNAHTVTQLINLLAALLLLIAFAMLSQRRILRLIHLFAAQGAVL
                     TLNTLLVAAALGQSHLYFSVALNLALKVVLLPWILHRLIDRLNLRWDVETLFNVPTIM
                     LVGIVLVIVSFNLALPISQLAGTITRGTLGIAMASVLISFLMMITRRKAVAQVIGFLA
                     MENGLFFAATSATYGMPMVVELGIALDVLVGTFIFGIFFFQIRETFDSLDIHHLERLK
                     ERE"
     gene            8500..9951
                     /locus_tag="VD791_13175"
     CDS             8500..9951
                     /locus_tag="VD791_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HXV08980.1"
                     /translation="MTEALLVLGVPLAGALILGAWGHRPWAAEANSSLSLLTLLAALA
                     LTARVVSMGPLTLLSELLFIDAFNVFLVTLTAFVGFTTALFSRVYMRVEQERGKLSTA
                     RLRLFHGMYQLFLFTMLLALIANNIGILWVAMEAATLATVLLVSLYRTPSGVEAAWKY
                     FILCGVGIAQALFGTILLYLAAERVLGAGGTALLWTHLNQVKGELEPTVLGIAFVFLL
                     VGYGTKVGLAPLHNWLPDAHAEGPTPISAVLSGLLLNVALYAVVRNKVLVDGALGTHA
                     AGNLMMGFGLLSVIVAAFFLSRQKDVKRLFAYSSIEHMGIATFAFGMGGPVASFAGLL
                     HMTAHSLTKSAIFFAVGHAAQKAGTQVIEDIRGLIHTSPAVGWGLMLGGIAILGIPPF
                     GVFASEFLILTTAMRDHPWATPILLLGLGVAFAAIFSRVQGMVFGETTTQRLPHPPAL
                     FPVFVHLALVLLLGLYIPPYLATWYRQAAAMIG"
     gene            9953..11509
                     /locus_tag="VD791_13180"
     CDS             9953..11509
                     /locus_tag="VD791_13180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HXV08981.1"
                     /translation="MKPEDLIANLQPLPGAMPVWRGTVDAAVWGATAQAVREQGGRLV
                     ALWGSDEAAAGFAVHAALAVRQGVLWLTLPLAGRQPEYPDLGAVFPAARRMQRAAYDL
                     LGVRATGADARKWLRHGAWPDGEFPLRKNFDAHPPAGAGRRFETVADDYPFVAVQGDG
                     VHEIPVGPVHAGIIEPGHFRFSVVGERVLRLEERLGYVHKGIEKRFESLGIHDGFRLA
                     GRVSGDSTVAYAWAYAMAAEAAAGLTPPPRALWLRALLLERERVANHLGDLGYLGNDA
                     GLAMGLAQFSRLKEEWLRASAALFGHRYLMDRVIPGGVACDLSAEGVHQIFREIAHLR
                     AEVARLKIIFDEHPGLQDRLIGTGRVVPELADRLGLTGLAGRASGMAWDLRVELPCAP
                     YDALEVAMATHRNGDVAARVTVRFEEVLASLDLCEQIVEHLPDGNVATLFPSPREGAS
                     GAGWVEGWRGEVFVALEAGANGALRRAHAHDPSWQNWPVLEHAVIGNIVPDFPLINKS
                     FNLSYSGVDL"
     gene            11510..12025
                     /locus_tag="VD791_13185"
     CDS             11510..12025
                     /locus_tag="VD791_13185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HXV08982.1"
                     /translation="MYLILKQIAKTGVVTEPAPGPDENLRVTAGRLQHAVLRRFAGAL
                     TIRHVDAGSCNGCELEIHATNNPYYNLEGLGIRFVASPRHADMLLVTGPVSRNMETAL
                     RRTYDATPEPRFVVAVGDCGCSGGIFGESYASCGRVSNVIPVDVAVPGCPPTPLAIMQ
                     GILTAIELPKR"
     gene            12046..12927
                     /gene="ubiA"
                     /locus_tag="VD791_13190"
     CDS             12046..12927
                     /gene="ubiA"
                     /locus_tag="VD791_13190"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HXV08983.1"
                     /translation="MTRAELLDRLDAYEKLMRLDKPIGTLLLLWPTLWALWLAAGGVP
                     HGKVLLIFVVGTLLMRSAGCAINDYADRDFDPHVARTRDRPLAAGRVKPVEALLLAAV
                     LALIAFALVLMLNRLTILLSVVAACLAASYPFTKRFFVLPQAYLGIAFGFGIPMAYAA
                     QLGQVPAVAWALLGANILWAIAYDTEYAMVDRDDDRKIGIRTAAILFGRCDVLAVMGF
                     HAAFLAVLAAVGRYLDLDWPYYAGLAAAVVLIGYQYRLIRTREPARCFRAFLNNNWVG
                     AAVFAGIALALALRHGL"
     gene            13035..14498
                     /gene="ubiD"
                     /locus_tag="VD791_13195"
     CDS             13035..14498
                     /gene="ubiD"
                     /locus_tag="VD791_13195"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041322210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HXV08984.1"
                     /translation="MRYHDLRDFLAQLEKQGELKRIAVEVSPRLEMTEICDRVLKQGG
                     PALLFEKPAGHTMPVLGNLFGTPKRVALGMGEDSVDALREVGKLLAYLKEPDPPQGLK
                     DAWDKLPVLKKVLDMAPRSRSSAPCQETVWEGRDVDLGRLPIQTCWPGDAGPLITWGL
                     TVTRGPLKKRQNLGIYRQQVIARDKVIMRWLAHRGGALDFRDHCTADPGQPFPVAVAL
                     GADPATILGAVTPVPDTLSEYQFAGLLRGARTELVKCLGSDLQVPASAEIVLEGVIHP
                     GETALEGPFGDHTGYYNEQERFPVFSIERITMRREPIYHSTYTGKPPDEPAVLGAALN
                     EVFVPLLARQFPEILDFYLPPEGCSYRLAVVRMRKQYPGHAKRVMFGVWSFLRQFMYT
                     KLIVVVDEDVDARDWKEVIWALTTRVDPARDTLIVEHTPIDYLDFASPVPGLGSKMGV
                     DATNKWPGETGRTWGRPIEMDAQVKQRVDALWDRLGL"
BASE COUNT         2073 a         4996 c         5071 g         2470 t
ORIGIN      
        1 ttcgctgaag cgactcagct cggtgagcgc ctggcccagc agcttccacg ccgccgaata
       61 gccgggatcc agggcgagcg cgcgctgcag gtgaccggcg gccatgccgg cgtccccggc
      121 cttcagatac tcgctgccca ggccgaagcg caacagcgcg ccgtcctgcc cggcggcgag
      181 cagcttttcc agattctcga tcgctgatct gccaggcatc tctggctgcg ctaaagctcg
      241 atgcgcgtgc ccagctcgac cacgcggttg gtggggatct tgaagaacac ggtcgcgctc
      301 gcggcgtttc gcgacatggc ggcgaacagc ttctcgcgcc acagcgccat cccgggcgag
      361 ggcgtgggca ccagcgtctg gcgcgacagg aagaacgagg tctccagcat gttgaactcc
      421 atgccgtgct gcgcggcggc cagcgccagc gccgccggga ggtcgggctc gtccttgaag
      481 ccgtagtgca cagtgaggcg gtagaagtcg tcgcccaggc tctcgagatt cacgcgctgt
      541 gcatcgggca cgtgcgggat gtcctcggag cgcaccgtca gcagcaccac ccgctcgtgc
      601 agcaccttgt tgtggttcag gttgtgcagc agcgcgtgcg gcacgccctc ttggctggcg
      661 gtgaggaata ccgccatccc aggcacgcgc accggcggat gctcggtgat gctcttgatg
      721 aacggctgca gcgcgatcgc ccccgggcgc agccgctcga acaggatctc gcggccgcgc
      781 ttccaagtgg acagcaggac gaacacgacc acgcccacca ccaccgggaa ccagccgcct
      841 tccaggatct tcagggagtt ggcgctgaag aaggaaagat ccaccgccag gaacaaggcc
      901 gagatcggcg ccgcgaggac aagcggccag tgccacagct tgcgcatgac gaagaagatc
      961 aggatcgagt cgatcgccat ggtggcggtg accgcgatgc cgtacgcgcc cgccagattg
     1021 ctcgacgacc cgaaccccag caccagtccg atcaccgcca ggtacaggcc ccagttgatc
     1081 catggcaggt acacctggcc gatctcgcgc tcggaggtat gctcgaccat caagcgcgga
     1141 cagtagccca gctggatcgc ctggcgggtg agcgagtagg cgcccgtgat cacggcctgg
     1201 gacgcgatca cggtggcgag cgtggcgagc accaccatcg gcagcagccc ccacgcgggg
     1261 accaggtgga agaacggatg ctgcacggcc tgcggttcgc gcaggagcaa cgcgccctga
     1321 ccgaagtagt tgatcagcag cgcggggaac acgaaccaga gccaggccag gcggatgggg
     1381 cgcttgccga agtgccccat gtcggcgtac aaggcctcgg caccggtgat cgcgagcacg
     1441 accgcaccca gcacgagaaa cccgagcgtg ccgttgctga cgaagaacct cacgccccaa
     1501 tacgggttga gcgcctccag cacgctgggg taatgccaga tgctgatcag gccgagcagg
     1561 gcgagcacgg cgaaccagag ggtcatgacc ggcccgaaga atgcgccgac gcgcccggtg
     1621 ccggtctgct gcaccacgaa cagaccggtg atcacggcga gcgtcaccgg gatcacgtac
     1681 ggcttgagca ccggcgtgat cacctccagg ccctcgaccg cggacagcac cgagatcgcc
     1741 ggggtgatca tgccgtcgcc atagaacagg gcggcgccga acatgcccag cacgatcagg
     1801 agccagcgca ggcgtggctc gccgtcgaga gagcggcgca ccagggccat cagcgccatg
     1861 atgccgcctt cgccgcggtt gtccgcgcgc atcatgagca gcacgtactt gagcgtgacg
     1921 atgctgaaca gcgcccacgc catcaccgag agcacgccga ccacgttgtc gtgcaccacc
     1981 ggcagattgt gcgtgccgcc gaaggattcc ttgatggcgt acaggggact ggtgccgatg
     2041 tcgccgtaga ccacgccgac ggccccgatc gccagaccca gcacaccccg ccgcgtgtgg
     2101 gaaacgggtt gatcgccgtc ggcctgttgc tgttgttcca cggaggttcg gtctgcgttg
     2161 cggcggcgca tcatagcacg ccgcgaggcg cgcacgagcg cggccggcgg cgcggttgtt
     2221 gtcgcatgcg cttgggcata ccattgcagc gctcaccagc gggcgggaac catggcgaaa
     2281 caggcgatac acaccgacga ggcgccgcaa ccgatcggca tctattccca ggccgtgcgc
     2341 gccggcaata cgctctacct ctccgggcag atcgggctcg atccgcgcac catgctgctg
     2401 gtcagcggca tcgacgcgca gatccaccag gtgttcctca acctgcgcgc ggtggcggcc
     2461 gctgccggag cgagcctcga cgacgcggtg aagttcaccg tcttcctcac cgacatgacc
     2521 gacttcccgc gcgtcaacga gatcatggcc gggtacgtga gccagcccta tgcggcgcgc
     2581 tccgccgtgg ccgccgcagg cctgccgcgc ggcgccctgg tcgagatcga ggcggtgctg
     2641 gtcgtcggtt gatcctggcg ggatgagccc gcagcgccgc gccggcggcg agccgggaat
     2701 cagcgtcccc gacgccgcgc gcaagcagct cgacaagctc ggtctgagca gcgcgctcga
     2761 cctcgcgctg cacctgccgc tgcgctacga ggacgagacc cggcttgcgc gcctgcgcga
     2821 cgtgcgcgcc ggcgacaccg cccaggtcga ggtcgaggtg ctcgccgccg agatccagtt
     2881 ccgccccaag cgcatcctgg tgtgccgcgc gcgcgacggc gacgaagagc tgacgctgcg
     2941 ctttttccac ttctacccca gccagctgcg ccagctcgct gccggtgtgc gcctgcgggt
     3001 gatgggtgag ctgcggcgcg gcttcttcgg cgccgagatg atccacccgc gctggcgcgt
     3061 ggtgcgcggc ggggaaccgc tgccggagtc gctcacgccg atctacccca gcaccgccgg
     3121 cctggctcaa ccgctgctgc gcaagctcat cgggcgtgcg ctggccgcgg ccgatctgtc
     3181 cgacccgctg cccgacgcgc tgcggacgca cttgcggctg gcgccgttcg aggacagcat
     3241 ccgctatctg cacaacccgc cgcccgacgc gccgcaggcc gcgctgcagg aacgcacgca
     3301 tccggcctgg cgccgggtca agttcgacga gctgctcgcg cagcaactgt cgatgcggct
     3361 gcatcaccgc cggcggcgct cggcgcgcgc cccggcgctg gatccgccgg ggccgctgtc
     3421 cgcgcgcctg ctcgcgcagc tgccgttccg cctgacgcgc gcgcagaagc gtgtgctcgc
     3481 cgagatccgc gccgacctgg cgcagccgca tccgatgcaa cggctgctgc agggcgacgt
     3541 gggcagcggc aagaccatcg tcgccgccct ggccgcgctg caggcggtgg agagcgcgcg
     3601 ccaggtggcg gtcatggcgc cgaccgagat cctggccgag cagcaccacg ccaagctcgg
     3661 cgcctggctt gcgccgctcg gcgtggaggt ggcctggctg gccggcagcc tgacgaagaa
     3721 ggacaagcgc gcggcgctcg cccgggtgga atcgggcgcc gcgcaggtgg ccgtcggcac
     3781 gcacgcgctg ttccaggacc aggtcgcgtt ccgcgatctg gcgctggccg tggtggacga
     3841 gcagcaccgc ttcggcgtgc atgagcgcct ggcgctgcgc ctcaagggca cgctcgaaag
     3901 cggcgagttg cagccgcatc aattgatgat gagcgccacg ccgatcccgc gcacgctggc
     3961 catgagctat tacgccgatc tcgatgtatc ggtgatcgac gagctgccgc ccgggcgcac
     4021 cccggtggtc accaagctgc tctccgagtc gcgccgcgag gaggtcaccg cgcgcgtgcg
     4081 cgacgcttgc atggccgggc gtcaggccta ctgggtgtgc ccgctgatcg aggagtccga
     4141 ggcgctccag ttgcagaccg cgatcgacac cttcgaaggg ctgcggcgca ccttcccgga
     4201 gttgagcgtc ggcctggtgc acggacggct gtcgagcgcc gacaagacgg cggtgatgga
     4261 agcattcgag gccggccgcg tgcagctgct ggtggccacc agcgtgatcg aggtcggcgt
     4321 ggacgtgccc aacgcctcgc tcatggtgat cgagcacgcc gagcgcatgg ggctgtcgca
     4381 gatgcatcag ctgcgcgggc gcgtggggcg cggggcgcag gccagcgtgt gcatcctgct
     4441 gtaccaggag ccgctgtcgg acacggcgcg cgagcggctg cgcatcatgt acaaggtgag
     4501 cgacggcttc gagatcgcgc gtcacgacct gcgcatccgc ggcccgggcg agctgctcgg
     4561 cgcgcgccag agcggcgtgc cgatgctgcg cttcgccgac ctcgaagccg acctcgatct
     4621 gctcgacgcc gcgcgcgggg ccgccgacgg attgctgcaa gacgcgccgg agcgcgcgcg
     4681 cgcgcatctg cagcgctgga tgggaggaag gcaggagtac ctgcgcgtgt gatccgaagg
     4741 gcgcgcggtg tcgatcgcgc agtaccgatc acgcctttcg gttatgattg ccgccggctc
     4801 ctcctctgcc gaacaacaag ctggccatcg tgccgctgct gcacgtcgtt ctctccgtcg
     4861 ttgcgtactg gatcgcgctg gggctgatcg gcctcgccgt cccgctggag cggggtgcgc
     4921 acgcgcggct gttctttccc ctgagcgcac tgggcgccgg ggtgctcgcc gccgccggtt
     4981 tcgtcgggct cggcgcctgg cccgaagcgc tggtgctgcc gctcgggctg cccgacctgc
     5041 cgttccacct gcgcctggac ccgctgtccg ccttcttcct gctgttgctc gggacggccg
     5101 cggcgggcgt gtcgctgttt tccgcgggct acttcgagcg tggcggcagc ggggacatcg
     5161 gtctgttgtg cctgcagtac cacgtgttcc tcgcttcgat ggtgatggtg ctgctggccg
     5221 acgacgcgta cctgttcatg gtggcctggg agtcgatggc gctcgcctcc tacttcctgg
     5281 ttaccaccga tcaccgtgtc gccgagatcc gcagcgccgg gttgctgtat ctggtggttg
     5341 cgcacctcgg cgcgatcgcg atcctgctcg ccttcggggt gctgcagctc gggcagtgga
     5401 gctacacctt cgcggcgatg cgcgacaacg cgctgacgcc tttctgggcg gcggtggctt
     5461 ttctgctggc gctgatcggc ttcggcgcca aggccggctt cctgccgctg cacgtctggc
     5521 tgcccgaggc gcatcccgcc gcgccgtcgc cggtgtcggc gctgatgagc ggcgtcatgc
     5581 tgaagaccgc ggtgtacggc ctgctgcgcg tgagcttcga tctgctgcag ggatacacct
     5641 ggtggtgggg cgtggcggct ctgtcggtgg ggctggtaac cgccttctac ggcgtggcct
     5701 tcgccgccgt gcagacggac atgaagcgcc tgctcgccta ttcttcgatc gagaacatcg
     5761 gcatcgtgct ggccggcctt ggcctggctc tgacttttca tgcctacaac atggagctgc
     5821 tggcggcgct ggcgctggcg gccagcctct accactgcct gaaccacgcg ctgttcaaga
     5881 gcctgttgtt cctggccacc ggctcggtga tgcatgccac gcggcagcgc agcctgggca
     5941 agctgggcgg cctgatccgg cgcatgccct gggtggccgg cctggcgctg gtgggcacgc
     6001 tggcgatcgc cgggctgccg ccgctcaacg gtttcgtctc ggagtggatg ctgtttcagg
     6061 cgttcctgct cacgccgggg ctgcccaact cctatctgaa catgttcgtc ccggtggcag
     6121 ccgccgcgct ggcgctcacc gtggcgctgg ccggctatgt gatggtcaag ttctacggca
     6181 tcgtgtttct cggcctgccg cgtgaggaaa gcctggagga cgcgcacgac gccgggccct
     6241 atgagcgcgc cgggctcatc tggctggcgc tgtggtgcgt ggtgctgggc gtgacgcccg
     6301 tgtacgtggt ccagctcatg gacccgctca cgcacggcct ggtcggggga gcgctgggcg
     6361 agagcgtgcg ccgctccggc tggctgttcc tcaccccggt ggccccggaa cgcgcgagct
     6421 acgcgccgct cttgttcctg ctggtcatcg tcggcgtggt gctgctcacc ttcctggtgg
     6481 tgcgccgact gtatcacggc cgcgtgcggc gggcgcccgc ctgggactgc ggcttcgccg
     6541 gcctgaacgc gcgcatgcag gacagcgccg aggggtacgg ccagccgatc aagcagatct
     6601 tcgagccgtt cttccggctc gagcgccacc accccagccc gttcgatgcc cagccgcgct
     6661 acttctcgcg catcgacgac cgcctgtggt actggctcta cctgccggtg gtgcgcctcg
     6721 cggagcgcgt gtcgtccctg ttcgcggtcg tgcagcacgg ccgcatcgcg ctgtacctga
     6781 tctacagctt cgccacgctg ctgctgctgt tgctgctgat tcgctggatc ggcgcggcat
     6841 gaactggacc gacctgctgt cgcagctgtt ccagaccctg ctggcggcgc tgctcgcacc
     6901 gctggcgctg ggctgggtga accagtgccg cgcgtggctg cagaatcgca gcgctcccga
     6961 cctgctgcaa ccctaccgcg tgctgttcaa gctgctggag aaggacgcgg tgctcgccgt
     7021 caatgcgtcc tggctgttcc gtcttgcccc ctacgtcgtg ttcgcctgca tgctgctcgc
     7081 tgccagcatc gtcccgctgg tggcgaccga tctgccgttt gcgcaggccg ccgacgtgat
     7141 cgcgctggtg ggactgttcg cgctggcgcg ggtgttcatg gcgctggcgg cgatggacgt
     7201 gggcaccgct ttcggcacgc tcggctcgcg ccgtgagatg ctgatcgcca gcctggccga
     7261 gcccgcgctg ctgatggtgt tcttcacccc cgccctgatc ggccagtcca ccgcgctcgc
     7321 caccatcgtc gagcagcttg cgcagcgcga gctgcagctc aatcccagca tggcgttcgc
     7381 cgcggtcgcc ttcgtcatgg tgctgctcgc cgagaacgcg cgcgttcccg tggacaatcc
     7441 ggccacgcac ctggagctca ccatggtgca cgaggccatg atcctggagt actcggcccg
     7501 gcacctggcg ttgatggagt gggcgcacgc gctcaagctg acggtgtacc tcgccatcgg
     7561 catcgcgctg ttcctgccct ggggcatcgc ccgggccggc gactggggga tgatgcccgc
     7621 cgcgatcctg tggttcgcgg cgaagctggc gttgctgggc gccgggctgg ccctgctgga
     7681 gacggtgtcg gccaagatgc gcatctttcg cgtgcccgag ttcctgggta tggccttcat
     7741 gctcgccgtg ctgggcatgc tgatccactt tctgctggaa gcatgagcca ccgagcatgg
     7801 cagcccgcgg tggcagaggg cgcagcatga gggccgcgtg aacgcgcaca ccgtcaccca
     7861 gctcatcaac ctgctggcgg cgctgctgct gttgatcgcc ttcgccatgc tgtcgcagcg
     7921 gcgcatcctg cgcctgattc acctgttcgc ggcgcagggc gcggtgctga cgctgaacac
     7981 gctgctggtc gccgcagcgc tcggccagag ccacctgtac ttttcggtgg cgctcaacct
     8041 ggcgctcaag gtggtgctgc tgccatggat cctgcaccgc ctgatcgacc ggctgaacct
     8101 gcgctgggac gtggagacgc tgttcaacgt gccgaccatc atgctggtcg ggatcgtgct
     8161 ggtgatcgtg tcgttcaacc tggcgctgcc gatctcgcaa ctcgcgggca ccatcacgcg
     8221 cggcacgctc ggcatcgcca tggcgagcgt gctgatctcg tttctgatga tgatcacgcg
     8281 tcgcaaggca gtggcccagg tgatcggctt cctggcgatg gagaacgggc tgttcttcgc
     8341 cgccaccagc gccacttacg gcatgccgat ggtggtggag ctcgggatcg cgctggacgt
     8401 gctggtgggc acgttcatct tcggcatctt cttcttccag atccgcgaga ccttcgacag
     8461 tctcgacatc catcacctgg agcggctcaa ggaacgcgaa tgaccgaggc gctgctggtg
     8521 ctcggagtgc cgctggccgg ggccctgatc ctgggtgcgt ggggccaccg cccgtgggcg
     8581 gccgaagcca acagctcgct gagcctgctc accctgctgg ccgcgctggc gctgaccgcg
     8641 cgcgtggtgt cgatgggccc gctcacactg ctctccgagc tgctgttcat cgacgcgttc
     8701 aacgtctttc tggtgacgct gaccgccttc gtcggtttca ccaccgcatt gttcagccgc
     8761 gtgtacatgc gcgtggagca ggagcgcggc aagctctcga cggcccggct gcgcctgttc
     8821 cacggcatgt accagctgtt cttgttcacc atgctgctgg cgctgatcgc caacaacatc
     8881 ggcatcctgt gggtggcgat ggaagcggcc accttggcca ccgtgctgct ggtgagcctg
     8941 taccgcaccc ccagcggcgt cgaggctgcg tggaaatact tcatcctgtg cggcgtgggc
     9001 atcgcgcagg cgctgttcgg caccatcctg ctgtatctcg ccgccgagcg cgtgctgggc
     9061 gccggcggca cggcgctgct gtggacgcac ctgaaccagg tcaagggcga gctcgaaccc
     9121 accgtgctgg gcatcgcctt cgtgttcctg ctagtcggct acggcaccaa ggtcggcctg
     9181 gcgccgctgc acaactggct gcccgacgcg cacgccgagg gtcccactcc gatctcggcg
     9241 gtgctgtcgg ggctgctgct caacgtcgcg ctgtacgcgg tggtgcgcaa taaggtgctg
     9301 gtggacggcg cgctgggcac gcacgcggcc ggcaatctga tgatgggctt cgggctgctg
     9361 tcggtgatcg tggccgcctt cttcctgtcg cggcagaagg acgtcaagcg gctgttcgcc
     9421 tattcctcga tcgagcacat ggggatcgca accttcgcgt tcggcatggg cgggccggtg
     9481 gcctcgttcg ccggcttgct gcacatgacc gcgcactcgc tcaccaagtc ggcgatcttc
     9541 ttcgccgtcg ggcatgcggc gcaaaaagcc ggcacgcagg tgatcgagga catccgcgga
     9601 ctgatccaca cgagccccgc tgtgggctgg ggcctgatgc tgggcggcat cgccattctc
     9661 ggcatcccgc cgttcggcgt gttcgccagc gagttcctga tcctgaccac ggcgatgcgc
     9721 gatcatccct gggccacgcc tatcctgctg ctcggcctgg gtgtggcgtt cgcggccatc
     9781 ttctcgcgcg tgcagggcat ggtcttcggc gagaccacta cacaacggct gccgcatccg
     9841 ccggcgctgt tcccggtgtt cgtccatctc gccctggtgc tgctgctcgg gctgtacatt
     9901 ccgccctacc tcgcgacctg gtaccggcag gcggcggcca tgatcggctg acatgaagcc
     9961 cgaggatctg atcgcgaacc tgcaaccgtt gcccggcgcg atgccggtgt ggcgcggtac
    10021 ggtggacgcc gcggtgtggg gcgcgacggc gcaggcggtg cgcgaacaag ggggccgcct
    10081 ggtggcgctg tggggatcgg acgaagcggc ggctggcttc gcggtacacg ccgcgctcgc
    10141 cgtgcgccag ggcgtcctgt ggctgacgct gccgctggcg ggacggcagc ccgagtaccc
    10201 ggatctgggt gccgtgtttc ccgccgcgcg acgcatgcag cgtgccgcct acgatctact
    10261 cggcgtgcgg gccacgggcg cggatgcgcg caagtggctg cggcacggcg catggcccga
    10321 cggcgagttc ccgctgcgca agaacttcga cgcgcaccca ccggccgggg cggggcgccg
    10381 cttcgagacg gttgccgacg attatccctt cgtcgcggtc cagggcgacg gcgtgcacga
    10441 gatcccggtc ggccccgtgc atgcgggcat catcgagccc gggcatttcc gcttctcggt
    10501 ggtgggcgag cgcgtgctgc gcctcgagga gcgcctgggg tacgtgcaca agggcataga
    10561 aaagcgcttc gagagcctcg gcatccacga cgggtttcgc ctcgccgggc gggtgagcgg
    10621 cgattccacc gtcgcctatg cctgggccta cgccatggcg gccgaggccg ccgccggcct
    10681 cacgcctccg ccgcgggcgc tgtggctgcg cgcgctgctg ctggagcgcg agcgcgtcgc
    10741 caatcacctg ggcgacctgg gctacctggg caacgatgcc ggactggcca tggggctcgc
    10801 ccagttctcg cggctcaagg aagagtggct gcgcgccagc gccgcgctgt tcgggcatcg
    10861 ctatctgatg gatcgcgtga ttcccggcgg cgtggcctgc gacctgagcg ccgagggtgt
    10921 gcaccagatc ttccgcgaga tcgcgcacct gcgcgccgaa gtggcgcgct tgaagatcat
    10981 cttcgacgag caccccgggc tgcaggaccg gctgatcggc accggccgcg tcgtgcccga
    11041 gctggccgac cgcctggggc tgaccgggct ggccgggcgc gccagcggca tggcgtggga
    11101 cctgcgcgtc gagttgccct gcgcgcccta cgatgcgctg gaggtggcca tggccaccca
    11161 tcgcaacggc gacgttgcgg cacgggtcac ggtgcgcttc gaggaggtgc tcgcctcgct
    11221 cgacctgtgc gagcagatcg tcgagcacct gcccgacggc aacgtcgcca cgctgttccc
    11281 gtctccgcgc gagggcgcga gcggcgcggg gtgggtggag ggctggcgcg gcgaagtgtt
    11341 cgtcgccctc gaagccggcg cgaacggcgc tctgcgtcgc gcacacgccc acgacccgtc
    11401 gtggcagaac tggcctgtgc tcgagcatgc ggtgatcggc aacatcgtcc cggacttccc
    11461 gctgatcaac aagtcgttca acctgtccta cagcggtgtg gatttgtaga tgtatctgat
    11521 cctgaaacag attgccaaga ccggcgtcgt caccgagccc gcgcccgggc cggacgagaa
    11581 cctgcgcgtc accgccgggc gtctgcagca cgcggtgctg cgccgattcg ccggcgcgct
    11641 gacgattcgc cacgtggacg ccggctcctg caacggctgc gagctcgaga tccacgctac
    11701 caacaacccc tactacaatc tggagggcct tggcattcgc ttcgtcgcca gcccgcggca
    11761 cgccgatatg ctgctcgtca ccgggccggt gtcgcgcaac atggagaccg cgctgcgtcg
    11821 tacgtacgac gccacgccgg aaccgcgctt cgtggtggcg gtgggcgatt gcggctgcag
    11881 cggggggatc ttcggcgaga gctacgcgag ctgcgggcgc gtgtccaacg tgatcccggt
    11941 ggacgtcgcg gtgcccggat gcccgcccac cccgctggcg atcatgcagg gcatcctgac
    12001 cgcgatcgag ctgccgaagc gctgagcccc tgtggcgagg acgagatgac gcgggccgag
    12061 ctgctcgatc gcctggacgc ctacgagaag ctgatgcggc tggacaagcc gatcggcacg
    12121 ctgctgctgc tgtggcctac gctgtgggcg ctgtggcttg cggcgggcgg agttccgcat
    12181 gggaaggtcc tgctgatctt cgtggtgggc accctgctga tgcgctcggc gggctgtgcg
    12241 atcaacgact acgcggatcg cgacttcgat ccgcacgtcg cgcgcacccg cgaccggccg
    12301 ctggcggcag gccgcgtcaa gccggtcgag gccctgctgc tggcagcggt gctcgcgctg
    12361 atcgcgtttg cgttggtgct gatgctcaac cggctcacca tcctgctctc tgtcgtggcc
    12421 gcctgccttg ccgccagcta tccgttcacc aagcgcttct tcgtgttgcc ccaggcctat
    12481 ctcggcatcg ccttcggctt cggcatcccc atggcctacg ccgcgcagct cgggcaggtg
    12541 cccgcggtcg cctgggcgct gctcggtgcg aacatcctct gggccatcgc ctacgacacc
    12601 gagtacgcga tggtcgatcg cgacgacgac cgcaagatcg gcatccgcac cgcggcgatc
    12661 ctgttcggcc gctgcgacgt gctcgcggtg atgggtttcc acgcggcgtt cctggcggtg
    12721 ctggccgcag tcggccggta cctcgatctc gattggccct actacgcggg ccttgccgcg
    12781 gccgtcgtct tgatcggcta ccagtaccgg ctcattcgca cgcgcgagcc ggcgcgctgt
    12841 tttcgggcct tcctcaacaa caactgggtg ggcgccgcgg tgttcgccgg catcgcgctt
    12901 gcgctcgcgc tccggcacgg cctatagtcg ggcgcattca cacgcgagaa gggagttcgg
    12961 atggagctcg gattgcgtgg caaggcggtg ctcgtcaccg gcggcagcat catcggcaac
    13021 ccggtcctct gagcgtgcgc taccacgatc tccgcgattt cctcgcgcag ctcgaaaagc
    13081 agggggagct caagcgcatc gccgtcgagg tctcgccgcg cctggagatg accgagatct
    13141 gcgaccgcgt gctgaagcag ggcgggccgg cgctgctgtt cgagaaacct gccgggcaca
    13201 ccatgccggt gctcggcaac ctgttcggca cgccgaagcg cgttgcgctc ggcatgggcg
    13261 aggattcggt ggatgcgctg cgcgaagtcg gcaagctgct cgcctacctg aaagagccgg
    13321 acccgccgca aggcctgaag gacgcctggg acaagctgcc ggtgctgaag aaagtgctgg
    13381 acatggcgcc acgcagccgc agctcggcgc cgtgccagga gacggtgtgg gagggccggg
    13441 acgtcgatct cggccgtctg ccgatccaga cctgctggcc cggcgacgcg gggccgctga
    13501 tcacctgggg gctcacggtc acgcgcggtc cgctcaagaa gcggcagaac ctgggcatct
    13561 atcgacagca ggtgatcgcg cgcgacaagg tgatcatgcg ctggctcgcg catcgcggcg
    13621 gggcgctcga ctttcgcgac cactgcaccg ccgatcccgg gcagccgttt ccggtggcgg
    13681 tggcgctcgg cgccgatccc gccaccattc tcggcgccgt cacgccggtg cccgacacgc
    13741 tgtccgagta tcagttcgcc ggcttgctgc gcggcgcccg caccgagctg gtgaagtgcc
    13801 tgggcagcga cctgcaggtt cccgcgagcg ccgagatcgt gctcgagggc gtcatccatc
    13861 cgggggaaac cgcgctcgaa ggcccgttcg gcgaccacac cggctactac aacgagcagg
    13921 agcgcttccc ggtgttcagc atcgagcgca tcaccatgcg ccgcgaaccg atctaccaca
    13981 gcacctacac cggcaagcct cccgacgagc ccgcggtatt gggtgcggcg ctcaacgagg
    14041 tgttcgtgcc gctgctcgcc cgccagtttc ccgagatcct cgacttctac ctgccgccgg
    14101 agggctgcag ctaccggctg gcggtggtgc gcatgcgcaa gcagtacccc ggccacgcca
    14161 aacgcgtcat gttcggcgtc tggtccttcc tgcgccagtt catgtacacc aagctgatcg
    14221 tggtggtgga cgaggacgtg gacgcgcgcg actggaagga ggtgatctgg gcgctcacca
    14281 cgcgcgtcga tcctgcgcgc gacacactga tcgtggagca cacgccgatc gattatctcg
    14341 acttcgccag cccggtgccg gggctgggtt cgaagatggg cgtggacgcg accaacaagt
    14401 ggccgggaga gaccggccgc acatggggca ggccgatcga gatggacgcg caggtcaagc
    14461 agcgcgtgga cgccctctgg gaccggctgg ggctctgagc gcggccccgg gatctggccg
    14521 ccaggcgcgc gcagagctgg ccggcactag aacttgtagg tcacgccggc gctgatcaag
    14581 tccacgtcag cctcgaagtc accgccgacg
//
LOCUS       DASPGE010000404        34105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1044115, whole genome shotgun sequence.
ACCESSION   DASPGE010000404 DASPGE010000000
VERSION     DASPGE010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 34105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34105
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044115"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..392
                     /locus_tag="VD791_13200"
     CDS             63..392
                     /locus_tag="VD791_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV08985.1"
                     /translation="MPARTAIENLEKLLAAGKDGALLRFGLGSEYLKAGDAGMAAGHL
                     QRALALDPGYSAAWKLLGQALTELNRFSEALAAYRQGIEAAQRNGDLQAAKEMQVFAR
                     RVEKRAR"
     gene            complement(405..773)
                     /locus_tag="VD791_13205"
     CDS             complement(405..773)
                     /locus_tag="VD791_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08986.1"
                     /translation="MARQLNITYFADRDMLGLVSDEDYEQFKDLLLEALHKEWPKAQV
                     AVDDAEHASVEVEILNGDFPNAKAEQQAKSDVESRVHEIANDVIEEREWATEEEYEGY
                     DEDLDEDFEERRGRDEEEDY"
     gene            complement(1019..1094)
                     /locus_tag="VD791_13210"
     tRNA            complement(1019..1094)
                     /locus_tag="VD791_13210"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1059..1061),aa:Ala,seq:cgc)
     gene            complement(1153..1938)
                     /gene="lgt"
                     /locus_tag="VD791_13215"
     CDS             complement(1153..1938)
                     /gene="lgt"
                     /locus_tag="VD791_13215"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HXV08987.1"
                     /translation="MFVHPEFDPVAIQIGPVAIRWYGLMYLVGFVGGLLLARWRVRRH
                     PELGWSKHDTDDLLFYCILGVVLGGRLGYVLFYKLGEYLHDPIRILYLWEGGMSFHGG
                     FLGVILALAWFARRSRRHWFAVTDFLAPLAPIGLGAGRLGNFINGELWGRPTDVPWAM
                     IFPTVDSQPRHPSQLYEFALEGVLLFAILWLFAMRPRPLGAVSAVFLIAYGALRFLVE
                     FTREPDAFLGTLALGLTMGQWLSLPMLVGGVVMLVWAYRVRRG"
     gene            2125..3660
                     /locus_tag="VD791_13220"
     CDS             2125..3660
                     /locus_tag="VD791_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV08988.1"
                     /translation="MLRWIRAGVSDHPLEDKEDARQLLGDLADKGSFRALEELAAYLD
                     AVKTAERLKPIRAFEIVELLDRSARPFLRKLNYDYVTHSTRLTRFQQHRLWYAAHTFL
                     TQLAEGYRFCVAKFEVGAVGGAALKPLLPKIAARAIRACGAQLKWALLRYGPVEHQVW
                     EHLARLYTASEALGFAPWLLTLYRGERRESSCERELLRPLMLTVSAPDGLLPVQVELV
                     DRIVAQCADAFVLGARPGGALHYVIDLAATFPPGRLSPSVAVGPQTRFFGPGEAAARV
                     ARYVALIERSGRVPEEMNLGLQLEPPVVLDTLQHLARYWAASLPERREPRRQRTEHVT
                     VVHEFGEVVANAGGLFFESAFVSNDEEWVLENESRGGFGAFVPKALGGWLRVGCLVGV
                     RREEGVAWGVGIVRRVVGDEQGNRHVGIQMLATGGAAVTLFPARPDGGVHASDGELCI
                     LLPSTTTQSGEAILLLRPALFSARRTLEMQAYDRRYRLHPVAITEAGEDFEIARFRIE
                     ELT"
     gene            complement(3688..4581)
                     /locus_tag="VD791_13225"
     CDS             complement(3688..4581)
                     /locus_tag="VD791_13225"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HXV08989.1"
                     /translation="MPELPDIVVYLEALERRISGATLARVRLLSPFLLRTVAPPIGAV
                     DGKRVERLRRIGKRIVIGLEGELFLVLHLMIAGRLRWLAPDDKPPGRIALALFEFTGP
                     QAGTLALTEAGTKRRASLHLVQGEQPLAQFQAGGLEPLACDLAGFAQRLTRENHTLKR
                     ALTDPRLFSGIGNAYSDEILHRAKLSPVALTGALSAEQTERLYLATRATLADWTQRLR
                     SAAGVAFPDKVTAFREGMAVHGRFGQPCPACGAPVQRIVYAENETNYCARCQTGGRIL
                     ADRALSRLLKKSWPRDIDELE"
     gene            complement(4581..5276)
                     /gene="mtgA"
                     /locus_tag="VD791_13230"
     CDS             complement(4581..5276)
                     /gene="mtgA"
                     /locus_tag="VD791_13230"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HXV08990.1"
                     /translation="MRKKWRWSGRLTLLVLSVVLLYQLWIFAHVVWWNFFNPSASAFM
                     QQRLVSMQQKDPKAKLRHSWVGYSKVSLNLKRALVVAEDANFMEHEGFDWDAIQRAYA
                     QNLKKGRVVAGGSTISQQLAKNLFLSGRRTALRKLQEALITVMIEAVMSKRRIFEIYL
                     NIIEWGDGVFGAEAASRHYFKKSAANLDAWDAAYLAAMVPNPRYYDRNRNTAYLQSKT
                     NLILGRMPGAEVP"
     gene            complement(5273..6118)
                     /gene="aroE"
                     /locus_tag="VD791_13235"
     CDS             complement(5273..6118)
                     /gene="aroE"
                     /locus_tag="VD791_13235"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HXV08991.1"
                     /translation="MADRYAVVGNPVAHSKSPLIHAEFARQTGQDMEYGRLLVPLDGF
                     RAAVDAFRREGGRGLNVTLPFKLEAFALADRRSERALDAEAANTLKFDADSVFADNTD
                     GVGLLRDLEVNLRFPLAGRRILVMGAGGAVQGTLGPLIAAHPALLTVANRTLDKAQRL
                     VTRFGAAASASGTRLQASGYEALAGRGFDLVVNGTSASLLGTGLPGALPELPGGVFAI
                     GGLAYDMMYGNGLTAFLQHAQGLGAARLADGIGMLVEQAAESFFLWRGVRPHTAPVIA
                     QLKTL"
     gene            complement(6118..7047)
                     /locus_tag="VD791_13240"
     CDS             complement(6118..7047)
                     /locus_tag="VD791_13240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXV08992.1"
                     /translation="MPASPYRLNAGRVDLPEDLYTPDFPPPTARGPLGLALAGSLLFH
                     AVLLSIHFEYPDLTRFKGPPPTIEVVLVNSKSATRPRKAELLAQADLDGGGNTAERRR
                     AKSNLPAVPDMPSETQLALAARRVQQLEQEAQRLLTLLERSERAVNPTTLPIEQLIRA
                     DAEKLTDLPDRQLQIARLEAEIAREWQSYQELPKRKFVGARTQGVIYAEYVDEWRQRI
                     ERVGTANFPSEARERAEFGTALVTVAIRADGSVEKVEIDRSSGSSVLDAAVERIVALA
                     GPFKPFPEKVRHETDILHITRSWSFTRTDLLVQ"
     gene            complement(7103..8953)
                     /locus_tag="VD791_13245"
     CDS             complement(7103..8953)
                     /locus_tag="VD791_13245"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HXV08993.1"
                     /translation="MNVFYEEDGAFKVGAVLADHDTSLQVEAPHGKRSKVKAASVLLR
                     FEGPIGTFMEEAHKAAEAIDLDFLWECSPQQEFAHDTLARDYYGRAPSALESAALLLR
                     LHGAPMYFYRKGKGHYKSAPPEALKAALASVERKRQQALQQARYLEQLSAFQLPPEFA
                     ARLPELLYRPDRNTIEVKALEEAASAAGMSTAHLLEKCGAIPSSEAYHLQRFLLEHFP
                     QGIDAPAAQEVIEPAELPLAPVQAFSIDDATTTEIDDAFSVRPRPAGGWEIGVHIAAP
                     ALAVAPGSSLDVSASARLSTVYMPGAKITMLPEAVIERYTLSEQRIVPTVSLYLELGP
                     DLRLLGTRSTVEKVRVAANLRHDALEEEFNEESLAAGRQDFPFAAELKLLWDLAGVLE
                     AGRGRQENQRTLNMDYSFYVEDGRVRIVQRKRGAPIDKLVSELMIFVNAEWGRLLAEA
                     GVPAIYRAQGNGKVRMSTVPSPHQGLGVSYYIWASSPLRRYVDLVNQRQLVAWIRGEA
                     PPYLPKSERMLTAMRDFEQAYEAYAEFQRGMERYWCLRWLLQEAVHSTSAEVIRENLV
                     KIDDIPLIAKAHGLPMLAPGARVQVEISDIDLLSLELRVQYRATLAAAVP"
     gene            complement(9107..9670)
                     /locus_tag="VD791_13250"
     CDS             complement(9107..9670)
                     /locus_tag="VD791_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HXV08994.1"
                     /translation="MLIYVHGFNSSPASTKARQLRAALEGLGRAGEFACPALPDRPAR
                     AIALLEAQMAQAPAAGITLVGSSLGGYYSTWLAERHGVRAVLVNPAITPHEGLRAYLG
                     PQTNLHIGERYQLTEQHLDELRALFVPRPTRLERYYLMVTTGDELLDYRVAVAKYAGA
                     RQLVVQGSDHGFADFEQHLDGVLEFAA"
     gene            complement(9672..10172)
                     /locus_tag="VD791_13255"
     CDS             complement(9672..10172)
                     /locus_tag="VD791_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HXV08995.1"
                     /translation="MRLSSRHRQWIYWTSALLFGSGALWLICHFFVRIEGEFGPAPHP
                     LESWALRVHGAAAMLALVVAGSLLPIHVRRAWHQRRNLLPGILLAGVLLLLTVSGYAL
                     YYFAGEEARPVISMLHWGVGLGVPALLVWHIAAGRATLPGMRAGSREAPAPATAPRRA
                     DDGRVA"
     gene            complement(10183..11025)
                     /locus_tag="VD791_13260"
     CDS             complement(10183..11025)
                     /locus_tag="VD791_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HXV08996.1"
                     /translation="MPDALELRRARPLLGTLVEIAARASEPEPLACAIEAAFATVAQV
                     HRLMSFHARDSDVSRLNRWAWRTALRVHPWTYAVLQEAQRVARASGGLFDVSVARILV
                     ARRLLPAPRGARVPDRRACCRDIRLLSGFRVRFDRPLLLDLGGIAKGFAVDRAVQRLR
                     SAGVESALVNAGGDLRCFGAAVQRVHVRDPRAPWRLLPLAELSNGAIATSAAYFTRQR
                     RAGRDVCAQVDPRGGRGVPAGSVSVAARRCITADAWTKVLLLGGARFLGRARRAGAIG
                     RLLG"
     gene            complement(11025..11582)
                     /locus_tag="VD791_13265"
     CDS             complement(11025..11582)
                     /locus_tag="VD791_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HXV08997.1"
                     /translation="MSDFIHWLVPAAIAPAAIAASTVAQHCYAAQYLTLEQAQAAIFP
                     EAREFTAATVQLSREQARGIEQLGGTQVRVAEQRVWEARSEGRLLGWFIVDEVYGKHE
                     LITYAVGLNADGSVRQIEVMDYRETYGYEIRNAAWRRQFVGKRHGDSLKLDQDIRNIS
                     GATLSCRHIAEGVRRLLALHEMALR"
     gene            complement(11660..12706)
                     /locus_tag="VD791_13270"
     CDS             complement(11660..12706)
                     /locus_tag="VD791_13270"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HXV08998.1"
                     /translation="MNRRHPAADRSGRRRFLKIAAAAVGSAVLPTRPATSAGMRHVQR
                     DFLLGADTEIQLYHPDAQAARAAIARCFAEVRRLENIFSLEQPASILSQLNRNGRLEH
                     PPAELVALLERAHDFGRATSGAFDVTVQPLWELFVRHFAANRADVDGPTAADVEQALS
                     RVDYRQIQVSPERIALGRNGAAITLNGVAQGFITDRVAETLHAHGFEHVLVNMGEMRA
                     LRGHADGTPWRIGIADPDRPWHSFLTLPLVARAIATSGGYGTAFDVGGRHHHLFDPRS
                     GGSANYYRSVSVIAPDATTADALSTGLSALPLEAALAVVDAHPDIGALFLGNDGGLIR
                     RGAIPRADGRASRT"
     gene            complement(12703..13254)
                     /locus_tag="VD791_13275"
     CDS             complement(12703..13254)
                     /locus_tag="VD791_13275"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HXV08999.1"
                     /translation="MIRGTTLVLFTAALLSLLSPDALLPARAMAAEVYLPAETFLAES
                     FDGNVPKPDVLWISGETRDAVHKILGHSPGAVRVRYWARGARTAWILEEIGKTQPITT
                     GIVVDQGAIARIAVLIYRESHGWEVRYPFFTAQFKGATLSATHQLDRNIDGISGATLS
                     VRALTRLARVALLLDARVRESAR"
     gene            complement(13304..14614)
                     /locus_tag="VD791_13280"
     CDS             complement(13304..14614)
                     /locus_tag="VD791_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV09000.1"
                     /translation="MGCLVDPSGRVRKLLKGRGKTMKSKKGQGLSWIHLAVGVLVAGA
                     ASAQDQPPSQEEMWRVIQEQQRAMEEQKREIERLKTQLDQTDEKVEATGERVEEVAAK
                     DPGKGGMVEGWWNKTTIGGYGELHYNGGLRDEIDFHRFVLFFGHQFSDRIRFFSEVEL
                     EHSLAGDDLPGEVELEQAYVEFDFSERTQAKVGLFLLPIGILNETHEPPTFFGVERNI
                     VETNIIPTTWWEGGVAGRAILGRGFSADVAYHSGLEVSTTGSGAYLIRGGRQKVAEAP
                     AEDPAVTGRLKWTGVPGVELAVSAQYQDDLTQGAQDVHAVLLETHAAIRRGPWWLRAL
                     YARWDLDGDQPEAIGRDEQYGWYVEPGYIFSTRFGDLGLFARYAETDNTAGDTTDSKL
                     KETTVGLNYWPHPQVVLKADYQFQSVPAGMVDDDRVNLGVGFMF"
     gene            complement(14807..16156)
                     /gene="mpl"
                     /locus_tag="VD791_13285"
     CDS             complement(14807..16156)
                     /gene="mpl"
                     /locus_tag="VD791_13285"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HXV09001.1"
                     /translation="MHIHILGICGTFMGGIAVIAKQAGHRVSGCDANVYPPMSTQLQA
                     QGIALIEGWDPKQLDLKPDLFVIGNVVSRGNALVEAILGRNLPYVSGPQWLHENVLRD
                     KWVLAVAGTHGKTTTSAMLAWVLEHAGLTPGFLIGGVPVDFEVSARATDSPFFVIEAD
                     EYDTAFFDKRSKFVHYRARTAILNNLEYDHADIFPDLAAIETQFHHLVRIIPGNGLIV
                     ADGREESLRRVLARGCWTPVEYLGVEEGWNAVPAADGSFEVRLRGQPQGVVRWDLLGA
                     HNRSNALAALAAARHAGVPVPVGIEGLGRFRNVKRRLEVRGAVNGITVYDDFAHHPTA
                     IETTIAGLRARIGERRILAVLEPRSNTMKMGAMKDSLAPSLRGADLVFCYTNRIGWDA
                     AGALRTLGAKVACHDDLDRLVQAIVAHARAEDHVLVMSNGGFGGIHTKLLQALSEGR"
     gene            complement(16159..17061)
                     /locus_tag="VD791_13290"
     CDS             complement(16159..17061)
                     /locus_tag="VD791_13290"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HXV09002.1"
                     /translation="MSVFPALRTAEERFAALPDFPYPSHYLETLPGYQGLRMHYVDEG
                     PRDAPVFLCLHGEPTWAYLYRRMIPVFVQAGQRVVAPDLFGFGRSDKPQDEGAYTFEF
                     HREALLALVRALDLRDIALVCQDWGGLLGLTLPLDLPGRFTRLLVMNTALGTGDAPLS
                     AGFTAWRAWVANNPDLSAGKLLARACPHLTPQEAAAYDAPFPDARYKAGVRRFPQLVP
                     ERLDAPGAELSRRARDWWSRDWHGVSFMGIGMTDPVLGPPVMRALHAHIRACPAPMEV
                     ADAGHFVPEWGGRIAANALAHFFG"
     gene            complement(17074..18231)
                     /locus_tag="VD791_13295"
     CDS             complement(17074..18231)
                     /locus_tag="VD791_13295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXV09003.1"
                     /translation="MLPRDPRPPPPEALAHSQALTRSIARDIQAAGGWIDFARYMERA
                     LYAPGQGYYVGGASKFGGEGDFVTAPELSPLFAATLARQVAEILAQAPGDVLELGPGS
                     GRLAADLLPALDDLDRLPARYRILEPSADLRARQQERMAQLPGPLARRVEWLERLPEA
                     LCGVVLANEVLDALPVHLIVWREDDIFERGVTWSEGRFQFEDRALPDGALRAQTQRVD
                     PPVPYASEIALAAPALVRSLAERLAQGVLLFIDYGFGEAEYYHPQRARGTLMCHYRHR
                     AHDDPFFVPGLQDITCHVNFSAVARAGVEAGLTLLGYTTQSHFLINLGITELLARTPA
                     EQAGRYLPLAAQAQRLLSPSEMGESFKAIALGRGMGSRLAGFARGDLGRLL"
     gene            18290..19030
                     /locus_tag="VD791_13300"
     CDS             18290..19030
                     /locus_tag="VD791_13300"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF006598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HXV09004.1"
                     /translation="MAEKVALITGGARRVGAAICRLLHASGVDLMVHYRRSADAARAL
                     QSELNAARADSVALVQADLLNLSSLPTMVAETVRQFGRLDVLINNASSFFPTPVGDIT
                     EQEWDDLVGTNLKTPMFLSQAAAPHLRRNQGCIVNIVDIHADRPMRNHVVYSLAKGGL
                     VALTRSLASELGPEVRVNGVSPGVIMWPEDDRWADELARQRIVQSTLLKRVGDPEDVA
                     RTVRFLVFDAPYITGQIIAVDGGRSIHL"
     gene            19044..19892
                     /gene="ttcA"
                     /locus_tag="VD791_13305"
     CDS             19044..19892
                     /gene="ttcA"
                     /locus_tag="VD791_13305"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HXV09005.1"
                     /translation="MDQQRRHYEANKLQKRLRRLAGQAIADFDMIRGGDRVMVCLSGG
                     KDSYALLDLLLSLQAHAPARFEVFAFHLDQKHPGYPAHVLPDYLTARGVPFRVVTQDT
                     YSVVKRVLPEGATLCGLCSRLRRGVIYRVAEEMGATRIALGHHRDDMLETFFLNLFFA
                     GRLKSMPPKLVSDDGRHVVIRPLAYVREQDLERYARIRGFPLVPCDLCGSQDNLQRQQ
                     VKQMLRDWDKRFPGRVQSMFRSLRNVVPSHLADTRLFDFAGLQPLGTPAVGGDLAFDE
                     DPLSVS"
     gene            19906..20337
                     /locus_tag="VD791_13310"
     CDS             19906..20337
                     /locus_tag="VD791_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HXV09006.1"
                     /translation="MTIVNFTPFSALLGGVLIGLAAALMWWLLGRVAGVSNILGQAFS
                     SDERELNWRLAFIAGLVLAGLGAKLFVPGAVHFSLPAGYGQLIVAGLLVGFGTQLGGG
                     CTSGHGVCGMSRLSPRSIVATLVFMAAGAFAVLGLRLAGTL"
     gene            20334..20777
                     /locus_tag="VD791_13315"
     CDS             20334..20777
                     /locus_tag="VD791_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HXV09007.1"
                     /translation="MKRVIASFLAGLLFGAGLIVAQMTNPSKVIGFLDITGNWDPSLA
                     FVMVGAIGVYGLAYWLSRARQARPFLVDGFNLPMRRQIDRSLVLGALIFGAGWGLSGF
                     CPGPALTSAGFGEPRVWVFIAAMLAGMMMYRLRGRAARAAPATGD"
     gene            complement(20797..21318)
                     /gene="fabA"
                     /locus_tag="VD791_13320"
     CDS             complement(20797..21318)
                     /gene="fabA"
                     /locus_tag="VD791_13320"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: protein motif:HMM:NF003509.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HXV09008.1"
                     /translation="MKANPKIAYDKREILAFCVHHPTLPRRLPAPPLLMFDRIPEIHT
                     DGGKHDAGWLVAEKDVRFDEWFFFAHFESDPVMPGILEVDAILQLCGFFLHHAGFGGY
                     GRALKVGKILFREQVRPHHKLVSYRIDFRRIAERPNPLAFAEGVAVVDGQLSAEVDGI
                     MVGLFPDLKYAYP"
     gene            complement(21315..22379)
                     /gene="lpxD"
                     /locus_tag="VD791_13325"
     CDS             complement(21315..22379)
                     /gene="lpxD"
                     /locus_tag="VD791_13325"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HXV09009.1"
                     /translation="MPALTAAQLLDDLKGTGLLVDRTGPDAMIEGVAAVETCGPGDLV
                     FADAEVYLETIRQRKPAVAVTRPDLAARLPGLAVLTARNVRLAQAHILRRYFDRDLRS
                     EGWPRVHPSAVVHETAVVAASAGIGPGAIVGAGARVGERCVVLAGAVIERDAVLGEDS
                     VVHPNAVVGWRCEIGRRCIIKAGAVIGSEGFGFAQDEMRRNHRVPQLGRVVVEDDVVV
                     GAACCIDRAAFEETRIGAGTILDNLCHIAHNVRIGEDCILTAMFCVAGSTTLGRRVIA
                     SGMTGILDHLTLCDDVVLVHRAGVANDIRAPGTYAGSPVQPLKDYMKNQAVARRLADL
                     RAELRELKTRLAALEAGRKA"
     gene            22534..22899
                     /locus_tag="VD791_13330"
     CDS             22534..22899
                     /locus_tag="VD791_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV09010.1"
                     /translation="MDPVRVARMDAYTMVSGMDKAAVERKVAELVRQGARAVTKVTPL
                     GRNWVATLEDPTARPDPDGCRVARLGLQIIVTGPDKTSVQKRAEALMKEGAALKSPPQ
                     ENTNGWSAVLDEAGMDRGA"
     gene            complement(23024..23854)
                     /gene="metF"
                     /locus_tag="VD791_13335"
     CDS             complement(23024..23854)
                     /gene="metF"
                     /locus_tag="VD791_13335"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020750024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HXV09011.1"
                     /translation="MGAKVFSFELFPPQTPEGLEKLRVTRRQLAQLKPAFFSVTYGAG
                     GTTRERTLGVALEIQAEGQQVAPHISCVASSAASVREILERYHAAGVRRIVALRGDLP
                     SGMADAGEFRYASDLVAFVRKEFGDTFHIEVAAYPEYHPQARTAQGDLLSFKRKVEAG
                     ADGAITQYFYNPDAYFAFVEECEAAGITVPIVPGIMPINRFAQLARFSDACGAEIPRW
                     IRRKLEGFGDDAASIRSFGLDVVTSLCDRLLRAGAPGLHFYTLNQAGPTTTIWQRLGL
                     "
     gene            complement(23892..25310)
                     /gene="ahcY"
                     /locus_tag="VD791_13340"
     CDS             complement(23892..25310)
                     /gene="ahcY"
                     /locus_tag="VD791_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HXV09012.1"
                     /translation="MSAVLQSKQATDFHVADLALADWGRKEIRIAETEMPGLMAIREE
                     FAPEQPLRGARIAGSLHMTIQTAVLIETLQALGAEVRWASCNIFSTQDHAAAAIAAAG
                     TPVFAYKGESLDEYWEFTHRIFAFGAGRGANMILDDGGDATLLVHLGTRAETEPACIG
                     RPANDEERALFAAIRRRLDQDSKFYSKLKAGIKGVTEETTTGVKRLYQMHKDGELGFP
                     AINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMVAGKIALVCGYGDVGKGSAQALRA
                     LSAQVWVAEVDPICALQAAMEGYRVVTMDYAADKADIFVSATGNIDVIAHEHMRRMKH
                     NAIVCNIGHFDNEIDVASLRQYQWENIKPQVDHIIFPDGKRIILLAEGRLVNLGCATG
                     HPSYVMSSSFTNQVMAQIELWANPEKYPVGVYVLPKHLDEKVARLQLKKLGAMLTELT
                     DKQARYIGVHKEGPYKPDHYRY"
     regulatory      complement(25330..25404)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     regulatory      complement(25435..25504)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(25543..26718)
                     /gene="metK"
                     /locus_tag="VD791_13345"
     CDS             complement(25543..26718)
                     /gene="metK"
                     /locus_tag="VD791_13345"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HXV09013.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQISDAVLDAILAQDKTARVAAET
                     LVNTGLVVMAGEITTSAVVDFQHVARETIRRIGYDNTEYGIDYKGCAVLVAYDKQSPD
                     IKQGVDQAQDDPLNQGAGDQGLMFGYACDETPQLMPLPIYLAHRLVERQSNLRRDGRL
                     PWLRPDAKSQVTVRYRDGKADSIDTVVLSTQHAPDVTLEKVRESVIEEIVKPVLPRHL
                     MKGEIKYLVNPTGRFVVGGPQGDCGLTGRKIIVDTYGGAAHHGGGAFSGKDPSKVDRS
                     AAYAARYVAKNIVAAGLASKCEIQVSYAIGVARPTSIMVTTFGTGKVADQKLEQLVQQ
                     HFDLRPRGIIQMLDLLRPIYAKTAAYGHFGREEPEFTWERSDKVEALRAEAGVRLHA"
     gene            27001..27867
                     /locus_tag="VD791_13350"
     CDS             27001..27867
                     /locus_tag="VD791_13350"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HXV09014.1"
                     /translation="MSRLGILLLWLLHFLPLALLAPIGRGIGLLAYLLIGKRRRIART
                     NLRLCFPELSDRQRERLLRAHFQCFGRALVESGIAWWGSPQRLRRVVRIEGQDQVERL
                     RGERFIALVPHFVGIELEGLRMAMDYRGVAVYVRQKDPFVDAFLKRKRERFAGTRMIL
                     RREGVKAILRALQGGQALQLSPDMDLGPKDAVFVPFFGVPAATVTVLSRMARLMKLRI
                     VPLVVRQLPGGRGYVARFYPAWEDYPTASVEDDTRHMNAFLEERIREMPEQYLWTHRR
                     FKTRPTGERGVY"
     gene            28024..28854
                     /gene="dapF"
                     /locus_tag="VD791_13355"
     CDS             28024..28854
                     /gene="dapF"
                     /locus_tag="VD791_13355"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HXV09015.1"
                     /translation="MRLKFTKMQGVGNDFVVIDAVNQRVRLDAPAARRLADRHFGVGC
                     DQILLIEAPRRADTDFYYRIFNSDGGEVQHCGNGARCFLRYVRDKGLTSKSAIRVETL
                     SGVIVPRLEPDGQVTVDMGPPQFEPARIPFLAERQALTYWLEVNGQPVEISALSVGNP
                     HAVQIVEDLQAAPVLTQGPLIERHPRFPEGANAGYMQVVDRGHIRLRVYERGAGETLA
                     CGTGACAAVVAGIGRGHLDGRVRVTTHGGDLTIAWTGERQPVLMTGPAESVFDGEIEL
                     "
     gene            28851..29513
                     /locus_tag="VD791_13360"
     CDS             28851..29513
                     /locus_tag="VD791_13360"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HXV09016.1"
                     /translation="MTIGTLRPEEVAKYLKDHPEFFEQYAELLSGIYLPHPYGGHAIP
                     LAERQVLGLRERSRALEGKLRELVQFGEENDTISDRVHRISLALLAAPDLAGVLQVLD
                     TGLREDFAVPEVALRLWPGQSDADRPELEPVSEEARVFADSLAGPYFTDRPMFESAEW
                     FGGGEVLRSFAYVALRAERPLGVLAMASPEPDRFTSDMGTLYVARLGELAGTAVRRHL
                     PD"
     gene            29523..30431
                     /gene="xerC"
                     /locus_tag="VD791_13365"
                     /pseudo
     CDS             29523..30431
                     /gene="xerC"
                     /locus_tag="VD791_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441249.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
     gene            30448..31632
                     /locus_tag="VD791_13370"
     CDS             30448..31632
                     /locus_tag="VD791_13370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HXV09017.1"
                     /translation="MAADLILKPGREKSLLRRHPWVFSGAVARVDGEPGMGETVALRA
                     SGGAFLAWGAYSPRSQIVARVWSWNEREPPGPALMRVLLARAIAMRGERLADAQAVRL
                     VHGESDGMPGLVVDRYGSVLVLQVSSAGAARWRDAIADALTALTGVHDIYERSDADVL
                     ALEGLAQQVGVLRGAAPHDPLIVEEHGLKLAVSALRGHKTGYYLDQRDNRRLVRDLSP
                     GREVLDCFCYTGGFTLSALAGGARRVTAVDSSAEALAAVRDNVQRNGLHAGQVECVEA
                     DVFEQLRRLRNESRRYQLIVLDPPKFAPTAALAQRAARGYKDINLLALKLLAPGGLLV
                     TFSCSGGVSRDLFQKIVAGAAADAGTDAQILRWLSAAPDHPVALGFPEGEYLKGLLCR
                     VP"
     gene            31649..32284
                     /locus_tag="VD791_13375"
     CDS             31649..32284
                     /locus_tag="VD791_13375"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXV09018.1"
                     /translation="MSETHFIVVRHGETAWNAEARIQGHLDSPLNEEGLAQALLVAER
                     LAHEQFDQFYCSDLGRVLQTVQPLVDRTGKRPLPTARLRERNLGVFQGLTGAECQRDW
                     PQDYARFHARDPDHAMPGGESIRQLVARVSAFFDEAAAAHAGQRVLAVTHGGVLDALY
                     RHATAMPLDRLRDFPIYNASLNWLRHSGAGWSVERWGDISHLTRDAALDDF"
     gene            32438..32716
                     /locus_tag="VD791_13380"
     CDS             32438..32716
                     /locus_tag="VD791_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV09019.1"
                     /translation="MLALLLTQQVLTAHPFAHRGGAADTQDDQSAPGERILCALCVAG
                     AASGSALPSAGHSPFASVAQVFEPTPITWAYRPPLARAFSARAPPILL"
     gene            32794..>34105
                     /locus_tag="VD791_13385"
     CDS             32794..>34105
                     /locus_tag="VD791_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXV09020.1"
                     /translation="MFKLKATAFAVAAGCAFPLCAQEDATMDEIRREIRQLKESYETR
                     IQALEQRLKDAEAAKAPSPAAAAPAAVTPAAKQVTDNAFNPAMSLILQGVYGDLKESP
                     EEYAVTGFADPEKIHPGTRGFSLGESELNLSANIDQLFYGNLLVAFEEGEVHVEEAWF
                     QTLALGHGLTVKGGRFFSGIGYQNMLHPHAWDFFDAALVQRSFLGNNYGDDGVQATWI
                     APLPVYLELGAEVGAGRLLPGTFEEHSELAEVDRDKNGAGAYALFARLGGDIGTSNSY
                     RVGVSYLDTSTDDQPFALADFDTRTGVSNEYSGDVHLYGVDLVWKWAPDGNPARRNFK
                     LVAEWMQLERKGDLVFDVGGAAVPDAFKLRQSGWYVQGVYQFMPTWRVGLRYDQLDQG
                     SSSLGANTGNIPGVDYDPERWSAMVDWTPSEFSRWRLQYSNDESR"
BASE COUNT         5192 a        11754 c        11760 g         5399 t
ORIGIN      
        1 aagatcccca ccaaccgcgt ggtcgagctg ggcacgcgca tcgagctcta gcgctgtaac
       61 cgatgcccgc cagaacggcc atcgagaatc tggaaaagct gctcgccgcc gggaaggacg
      121 gcgcgctgtt gcgcttcggc ctgggcagcg agtatctgaa agccggggac gccggcatgg
      181 ccgccggtca cttgcagcgc gcgctcgccc tggatcccgg ctattcggcg gcgtggaagc
      241 tgctgggcca ggcgctcacc gagctgaatc gcttcagcga agcgctggcc gcgtatcgcc
      301 aggggatcga agccgctcag cgcaacggcg acctgcaggc ggccaaggag atgcaggtgt
      361 tcgcgcgccg cgtcgagaag cgggcgcgct gacgttccgg ttcctcagta gtcctcttct
      421 tcgtcgcggc cgcggcgctc ctcgaaatcc tcgtcgaggt cctcgtcgta gccctcgtat
      481 tcttcctcgg tggcccactc gcgctcttcg atgacgtcgt tggcgatctc atgcacgcgc
      541 gattcgacgt cggacttggc ctgctgctcg gctttcgcgt tggggaaatc gccgttcagg
      601 atctcgactt cgacgctggc gtgctcggcg tcatccaccg cgacctgcgc cttgggccat
      661 tccttgtgca gggcttcgag gagcagatcc ttgaactgct cgtagtcctc gtcggacacc
      721 agtccgagca tgtcgcgatc ggcgaagtag gtgatgttga gctgtctggc catccgcctc
      781 tcctgactcg ggccgcggtg ccgggccgcc gcgggcccgg cgctcggctc cctggatgtc
      841 gaagctaaat caagcatagc atgagggttt tgggtgcgtc tcacgaccac gacaactccc
      901 cgccctccgt gctgcggcgg tgacattcct tcagcaagcc aaatgccaac gcggatgccg
      961 gtgcagcgat gcccggcgcg aagaaaaaag gcccgcgcgt cggcgggccg gggatgggtg
     1021 gtggagtgga aggggatcga accctcgacc ttcgcattgc gaacgcgacg ctctcccagc
     1081 tgagctacca ccccatcgcg gcttcgcccg gcagcccggg cgagggcgcg cattgtacaa
     1141 caaacgcccg gtctaccccc gccgcacgcg ataggcccag accagcatca ccacgccgcc
     1201 gacgagcatc ggcaggctca gccactgacc catggtcagc cccagcgcaa gcgttcccaa
     1261 gaatgcgtcc ggctcgcgcg tgaactccac cagaaagcgc agcgcgccgt aggcgatcag
     1321 gaacaccgcc gacaccgcac ccagggggcg cggccgcatg gcgaacagcc acaggatcgc
     1381 gaacagcagc acgccctcga gcgcgaactc gtagagctgc gacgggtgcc gcggctggct
     1441 gtccacggtg gggaagatca tggcccaggg tacgtcggtg ggacggcccc acagctcgcc
     1501 gttgatgaag ttgccgagcc gccccgctcc caggccgatc ggcgccagcg gagcgaggaa
     1561 gtcggtgacc gcgaaccagt gccgccggct gcgcctggca aaccaggcca gcgccaggat
     1621 cacgccgagg aaaccgccgt ggaaggacat gccgccttcc cacagataca ggatccggat
     1681 cgggtcgtgc agatactcgc cgagcttgta gaacagcacg tagcccagcc gtccgcccag
     1741 caccacgccg agaatgcagt agaacagcag gtcgtcggtg tcgtgcttgc tccagcccag
     1801 ctcgggatgc cggcgcacgc gccagcgggc gagcagcaac ccgccgacga aacccaccag
     1861 gtacatcaat ccgtaccagc ggatcgcgac ggggccgatc tggatggcaa cgggatcgaa
     1921 ctcggggtgg acgaacatgg gctcgcgcgg gggggcgggg gaggaattat acgggaccgc
     1981 tccgtgcgcc cggccccgcc tgcggccaag cggtcgacgc aagccggcgt ggactcgcct
     2041 cgtcgacgct gcgggcatcg cgagcggcgg tggccgtcaa tggcatggaa cttgcgccat
     2101 gcccgccctc atggagggca atccatgctc cgctggatcc gagccggcgt ctcggaccat
     2161 ccgctagaag acaaagaaga cgcgcgccag ctcctcggcg atctggccga caaggggtca
     2221 ttccgcgcgc tggaggaact tgccgcctat ctggatgcgg tcaagaccgc cgagcggctc
     2281 aagccgatcc gcgccttcga gatcgtggag ttgctcgacc gcagcgcgcg cccgttcctg
     2341 cgcaagctca actacgacta cgtcacccac agcacccggc tcacgcgctt ccagcagcac
     2401 cggctctggt acgcggcgca cacgttcctg acccagctcg ccgagggcta ccgtttctgt
     2461 gtggccaagt tcgaggtcgg cgcggtcggc ggcgcggcgc tcaagccgtt gctgccgaag
     2521 atcgccgcac gcgcgattcg cgcctgcggc gctcagctca agtgggcgtt gctgcgctac
     2581 gggccggtcg agcatcaggt gtgggaacac ctggcgcgcc tgtacaccgc ctcggaagcg
     2641 ctcggtttcg cgccgtggct gctcaccctc taccgcggcg agcggcgcga gtcgtcctgc
     2701 gagcgcgagc tgctgcgtcc gttgatgctg accgtatccg cccccgacgg actgctgcct
     2761 gtgcaggtgg agctggtgga ccggatcgtc gcgcagtgcg cggacgcgtt cgtcctgggc
     2821 gcgcgtcccg gtggcgcact gcattacgtg atcgatctgg cggccacctt tccgcccggc
     2881 cggttgtcgc cgagcgtcgc cgtgggcccg cagacgcgct tcttcgggcc cggcgaggcc
     2941 gccgcgcgcg tcgcgcgcta cgtcgcgctg atcgagcgca gcggccgggt gcccgaggag
     3001 atgaacctgg ggctgcagct ggaaccgccg gtggtgctgg acacgttgca gcacctggcg
     3061 cgctactggg cggccagcct gcccgagcgc agggaacctc gccgccagcg caccgagcac
     3121 gtcaccgtgg tgcacgagtt cggcgaagtg gtggccaacg ccggcgggct gttcttcgag
     3181 tcggccttcg tcagcaacga cgaggaatgg gtcctcgaga acgagagccg gggggggttc
     3241 ggcgccttcg tgccgaaggc gctgggtggc tggctgcgcg tgggctgcct ggtgggcgtg
     3301 cggcgcgagg aaggagtcgc ctggggcgtg ggcatcgtgc ggcgcgtggt gggcgacgag
     3361 cagggcaacc gccacgtcgg catccagatg ctcgccaccg gcggcgccgc ggtgacgctg
     3421 ttcccggcgc gcccggacgg cggcgtgcac gcgtccgacg gcgagttgtg catcctgctg
     3481 ccttcgacca cgacgcagag cggagaggct atcttgctgc tgcgtccggc cctgttttcc
     3541 gcgaggcgca cgctcgagat gcaggcttac gatcgccgct accggttgca tccggtagcg
     3601 atcaccgaag cgggcgagga cttcgagatc gcgcgcttcc gcatcgaaga gctgacctga
     3661 gcggcccggc tccgcgcttc agcacgccta ctccaactcg tcgatatcgc gtggccagct
     3721 cttcttgagc aggcgcgaca gcgcgcgatc ggccaggatc ctgccgccgg tctggcagcg
     3781 cgcacagtag ttggtttcgt tctcggcgta cacgatgcgc tgcaccggtg cgccgcacgc
     3841 cgggcagggc tggccaaagc gtccgtgcac cgccatcccc tcgcggaaag cggtcacctt
     3901 gtccgggaac gccacgcccg ccgcgctgcg cagccgctgc gtccagtccg ccagcgtagc
     3961 gcgcgtggcc aggtacagcc gctcggtctg ctccgcgctg agcgcgccgg tcaaggccac
     4021 cggcgagagc tttgcccggt gcaggatctc gtcggagtag gcgttgccga tgccgctgaa
     4081 caggcgcgga tcggtcaggg cccgcttcaa ggtgtggttc tcccgggtga gccgctgcgc
     4141 gaagcccgcc aggtcgcagg cgagcggctc caggccgccg gcctggaact gcgccagcgg
     4201 ctgctcgccc tgcaccaggt gcagcgacgc gcggcgcttg gtgccggcct cggtgagcgc
     4261 gagcgtgccg gcctggggtc cggtgaactc gaacagcgcc agcgcgatgc gcccgggcgg
     4321 cttgtcgtcg ggcgcgagcc agcgcagccg cccggcgatc atcaagtgca gcaccaggaa
     4381 caactcgccc tccaggccga tgacgatgcg cttgccgatg cgccgcagcc gctcgacgcg
     4441 cttgccatcc acggcaccga tcggcggggc gacggtgcgc agcagaaacg ggctcagcag
     4501 ccgcacgcgc gccagcgtcg cgcccgagat gcgccgttcc agcgcctcga ggtagacgac
     4561 gatgtcgggc agctcgggca tcacggcacc tcggcgcccg gcatgcgccc gaggatgaga
     4621 ttggtcttgc tctgcaagta ggcggtgttg cggttgcggt cgtagtagcg cgggttgggg
     4681 accatggcgg ccagatatgc cgcgtcccag gcgtccaggt tcgcagcgct cttcttgaag
     4741 tagtggcgcg aggccgcctc ggcgccgaac acgccgtcgc cccactcgat gatgttgaga
     4801 tagatctcga agatgcggcg cttgctcatc acggcttcga tcatgaccgt gatcagcgcc
     4861 tcctgcagct tgcgcagcgc ggtcctgcgc ccggacagga acaggttttt cgccagctgc
     4921 tggctgatgg tggaaccgcc cgccaccacc cgccctttct tcaggttctg cgcgtaggcc
     4981 cgctggatcg cgtcccagtc gaagccctcg tgctccatga agttggcatc ctcggccacc
     5041 accagggcac gcttcagatt gagcgagacc ttgctgtagc ccacccaact gtggcgcagc
     5101 ttcgccttcg ggtccttctg ctgcatgctc accagccgct gctgcatgaa ggcgctggca
     5161 gaggggttga agaagttcca ccacaccacg tgggcgaaga tccacagctg gtacaggagc
     5221 acgacgctca ggacgagcag cgtcaggcgc ccgctccagc gccacttctt tctcacagcg
     5281 tcttgagctg cgcgatgacc ggcgcggtat gcgggcgcac cccgcgccag aggaagaacg
     5341 attctgcggc ctgctccacc agcatgccga tgccgtcggc caagcgcgcc gcgcccaggc
     5401 cttgcgcgtg ctgcaggaac gccgtgaggc cgttgccgta catcatgtcg taggcgagcc
     5461 cgccgatggc gaacacgccg ccgggcagct cgggaagcgc gccgggcagg ccggtgccga
     5521 gcaggctggc cgaggtgccg ttcacgacca gatcgaagcc ccggccggcg agcgcttcgt
     5581 aaccgctcgc ctgcagccgg gtgccggagg cgctcgccgc ggcgccgaag cgagtcacca
     5641 ggcgctgcgc cttgtccagc gtacggttgg cgacggtgag cagcgccggg tgtgccgcga
     5701 tcagcggtcc cagggtaccc tgcacggcgc cgcccgcgcc catcaccagg atgcggcgtc
     5761 cggcgagcgg aaagcgcagg ttcacctcga gatcgcgcag caagccgacg ccgtcggtgt
     5821 tgtcggcgaa gacggaatcg gcatcgaact tgagcgtgtt ggcggcctcg gcgtcgagcg
     5881 cacgctcact gcggcgatcg gcgagcgcga aggcctccag cttgaacggc aacgtcacgt
     5941 tgaggccgcg cccgccctcg cgccggaagg cgtcgacggc ggcgcgaaag ccgtcgagcg
     6001 gcacgagcag cctgccgtat tccatgtcct gcccggtctg gcgcgcgaac tcggcgtgga
     6061 tcagcggcga cttgctgtgc gccaccgggt tgccgacgac ggcgtagcga tccgccatca
     6121 ctgcaccagc agatcggtgc gggtgaacga ccagctgcgc gtgatgtgca ggatatcggt
     6181 ttcgtgccgc accttctccg ggaacggctt gaacggcccg gcgagcgcga cgatgcgctc
     6241 gaccgccgcg tccagcacgc tcgagcccga ggagcggtcg atctcgacct tctccacgct
     6301 gccgtcggcg cggatcgcca ccgtgaccag cgccgtgccg aactcggcgc gctcgcgcgc
     6361 ctcggacggg aaattggcgg tgcccacgcg ctcgatgcgc tggcgccact cgtccacgta
     6421 ttcggcgtag atcacgccct gggtgcgcgc gccgacgaac ttgcgctttg gcagctcctg
     6481 atagctctgc cactcgcgtg cgatctcggc ctcgagcctg gcgatctgca gctgccggtc
     6541 gggcaggtcg gtgagcttct ccgcatcggc tctgatcagc tgctcgatcg gcagcgtggt
     6601 cgggttcacg gcgcgttcgc tgcgctccag cagggtgagc aggcgctgcg cctcctgctc
     6661 gagttgctgc acccgccgcg cggccagcgc gagctgagtc tccgagggca tgtccgggac
     6721 cgcaggcagg ttgcttttgg cgcgccgccg ctcggcggtg ttgccgccgc cgtcgagatc
     6781 ggcctgggcc agcagttcgg ccttgcgcgg gcgcgtggcc gatttgctgt tcaccagcac
     6841 tacttcgatg gtgggcggcg gccccttgaa acgcgtgagg tcggggtact cgaagtggat
     6901 cgacagcagc acggcgtgga acagcagcga ccccgccaga gcgagcccga gcgggccgcg
     6961 cgcggtagga ggcggaaagt cgggggtgta aagatcctcg ggcaggtcga cccgaccggc
     7021 gttcaggcgg tagggagaag cgggcaacgc gcgagccatg gcgtcatggg ttgcgccatt
     7081 gtatgcaagg ctcgggccac tgtcatggca cggccgccgc cagcgttgcc cggtattgca
     7141 cgcgaagctc cagcgacagc aggtcgatgt ccgagatctc cacctgcacc cgggccccgg
     7201 gtgccagcat cggcagcccg tgcgccttgg cgatcagggg gatgtcgtcg atcttgacca
     7261 ggttttcgcg gatcacctcc gccgaggtcg agtgcaccgc ctcctggagc agccagcgca
     7321 ggcaccagta ccgttccatg ccgcgctgga actcggcgta ggcttcgtag gcctgctcga
     7381 agtcgcgcat ggcggtcagc atgcgctcgc tcttgggcag gtagggcggc gcctcgccgc
     7441 ggatccacgc caccagctgg cgctggttca ccaggtccac gtagcgccgc agcggcgagc
     7501 tcgcccagat gtaataggac acgcccagcc cctgatgcgg cgagggcacg gtggacatgc
     7561 gcaccttgcc gtttccctgg gcccggtaga tggcgggcac gcccgcctcc gccagcagcc
     7621 ggccccattc ggcgttgacg aagatcatca gctcggagac cagcttgtcg atgggggcac
     7681 cgcgcttgcg ctgcacgatg cgcacccggc cgtcctccac gtagaagctg tagtccatgt
     7741 tcagcgtgcg ctggttctcc tgccgcccgc gcccggcctc cagcacgccg gccaggtccc
     7801 acagcagctt cagctcggcg gcgaagggga aatcctgccg cccggcggcc agggattcct
     7861 cgttgaactc ctcctcgagg gcatcgtgac gcagattggc cgccacgcgc accttctcca
     7921 cagtgctgcg cgtgccgagc agccgcagat cgggcccgag ctccaggtac agcgacaccg
     7981 tcggcacgat ccgctgctcg gacagcgtgt agcgctcgat gacggcctcc ggcagcatgg
     8041 tgatcttggc ccccggcatg tacaccgtgg acaggcgcgc cgacgcgctc acatccagcg
     8101 acgagccggg cgcaaccgcg agcgcgggcg ccgcgatgtg cacgccgatc tcccacccgc
     8161 ccgccggccg cgggcgcacc gagaacgcat cgtcgatctc cgtggtggtc gcgtcgtcga
     8221 tgctgaacgc ctgcaccggc gccagcggca gttcggcggg ctcgatcacc tcctgcgcgg
     8281 ccggcgcatc gatgccctgc ggaaagtgct ccagcaggaa gcgctgcagg tgataggcct
     8341 cggaggacgg gatcgcgccg cacttctcga gcaggtgcgc cgtggacatc ccggccgcac
     8401 tggccgcctc ctccagcgcc ttgacttcga tcgtgttacg gtcggggcgg tacagcagct
     8461 ccggcaggcg cgccgcgaac tccgggggga gctggaaggc gctgagctgc tccagatagc
     8521 gcgcctgctg cagggcctgc tgccgcttgc gctccacgct ggcgagcgcc gccttgagcg
     8581 cctcgggcgg cgccgacttg taatggccct tgcccttacg gtagaaatac atcggcgccc
     8641 cgtgcaggcg cagcagcagc gcggccgact ccagcgcgct cggcgcacgc ccgtagtagt
     8701 cgcgcgcgag cgtgtcgtgc gcgaactcct gttgcggcga gcactcccac aggaagtcga
     8761 gatcgatcgc ctcggccgcc ttgtgcgcct cttccatgaa cgtcccgatc gggccttcga
     8821 agcgcagcag cacgctggcc gccttcacct tgctgcgctt gccgtgcggg gcttccacct
     8881 gcagcgaggt gtcgtgatcg gcgagcacgg caccgacctt gaaggcgccg tcctcttcgt
     8941 agaagacgtt catgcgtggg gcgcgaggaa tgaggcgtgc ggcgggttcg gtgcgctggc
     9001 ggcccgagtt ggggcgcgca ttatacccgc accgcgcgcg ccggcgacgc gccgcatgcg
     9061 ggtgccagaa cgcgcgggac cgcgcggctt ccagcgcgca ggccggctag gccgcgaact
     9121 ccagcacgcc gtcgaggtgc tgctcgaagt cggcgaaccc gtggtcgctg ccctgcacca
     9181 ccagctgcct ggcaccggcg tacttcgcca ccgccacgcg gtaatcgagc agttcgtcgc
     9241 cggtggtgac catgagatag tagcgttcga ggcgcgtggg ccgcggcacg aacagcgcgc
     9301 gcagctcatc gaggtgctgc tcggtcagct ggtagcgctc gccgatgtgc agattggtct
     9361 gcggccccag gtaggcgcgc agcccttcgt gcggtgtgat cgccggattc accagcaccg
     9421 cgcgcacccc gtgcctctcg gcgagccagg tggaatagta gccgcccagc gagctgccga
     9481 ccagggtgat gcccgcagcc ggcgcctgcg ccatctgcgc ctcgagcagc gcgatcgcgc
     9541 gcgccggacg gtcaggcagc gccgggcagg cgaactcgcc cgcgcggccc agtccctcga
     9601 gtgcggcgcg cagctggcgc gccttggtcg atgccggcga gctgttgaac ccgtgcacgt
     9661 agatcagcat gtcatgcgac gcgtccgtcg tcggcgcgtc gcggggcggt ggcgggtgcg
     9721 ggtgcttccc gcgaaccggc acgcatgccg ggcagcgtcg cccgacccgc agcgatgtgc
     9781 cacaccagca gcgccggcac acccaatccg acgccccagt gcagcatgct gatcaccggc
     9841 ctcgcctcct cgccagcgaa gtagtacagg gcgtagccgc tcacggtgag caggagcaag
     9901 acgccggcga gcaggatgcc gggcaggaga ttgcgtcgct gatgccaggc gcgacgcacg
     9961 tgaatcggca gcagcgaacc cgccaccacc agcgccagca tcgccgcggc accgtgcacg
    10021 cgcagcgccc aggattccag cgggtgcggg gccggcccga actcgccctc gatgcgcacg
    10081 aagaagtggc agatcagcca cagggcgccg ctgccgaaga gcagcgcgga ggtccagtag
    10141 atccactgcc ggtgccgcga agacaagcgc atgccatggc cctcagccga gcagccgccc
    10201 gatcgcgccg gcgcgccggg cacgaccgag aaagcgcgcg ccgcccagca gcagtacctt
    10261 ggtccaggcg tcggcggtga tgcagcggcg cgcggccacc gaaacgctgc cggcgggcac
    10321 gccgcggccc ccacgcgggt cgacttgcgc gcaaacgtcc cgtcctgcgc ggcgctgccg
    10381 agtgaagtag gcggcgctgg tcgcgatggc gccgttgctg agctcggcaa gcggcagcag
    10441 gcgccatggc gcgcgcggat cgcgcacgtg cacccgctgg accgccgcgc cgaagcagcg
    10501 cagatcgccg cccgcgttca ccagcgcgct ctcgacgccg gcgctgcgca accgctgcac
    10561 cgcgcggtcc accgcgaagc ccttggcgat accgcccagg tcgagcagca gtgggcggtc
    10621 gaagcgcacc cgaaagcccg acagcagccg gatgtcgcgg cagcaggcac ggcggtccgg
    10681 tacgcgcgcg ccgcgcggcg ccggcaacaa gcgccttgcc accaggatgc gcgccacgga
    10741 cacgtcgaac agtccgccac tcgcccgcgc cacgcgctgc gcttcctgca gcacggcgta
    10801 ggtccaggga tgcacgcgca acgcggtgcg ccaggcccag cggttgagcc ggctcacgtc
    10861 gctgtcgcgc gcgtgaaagc tcatcaggcg gtgcacttgc gcgaccgtgg cgaacgccgc
    10921 ttcgatggcg cacgccaagg gctcgggctc gctggcgcgg gctgcgatct ccaccagcgt
    10981 gccgagcagc ggccgggcac ggcgcagttc cagcgcgtct ggcatcaacg caacgccatc
    11041 tcgtgcaggg ccagcaggcg cctgaccccc tctgcgatgt gccggcagga cagcgtggcg
    11101 ccactgatgt tcctaatgtc ctgatccagc ttcaggctgt cgccgtgacg cttgccgacg
    11161 aactggcggc gccaggcggc gttgcgaatc tcgtacccgt aggtttcgcg gtagtccatc
    11221 acttcgatct ggcgcacgct gccgtcggcg ttcaggccca cggcataggt gatgagttca
    11281 tgcttgccat agacctcgtc gacgatgaac cagcccagga gtcgcccttc ggagcgtgcc
    11341 tcccacacgc gctgctcggc cacgcgcacc tgcgtgccgc cgagttgttc gatgccccgc
    11401 gcctgctccc ggctgagctg caccgtcgcc gccgtgaact cgcgcgcctc ggggaagatc
    11461 gccgcttgtg cttgttccag cgtcaggtat tgcgccgcgt aacaatgctg cgccacggtg
    11521 ctcgccgcga tcgcagccgg ggcgatcgcg gcgggcacga gccaatggat gaagtcggac
    11581 atggtcgaga gtacagattc tagcctgtgc ggcgccgatt ctcacgcgcc gccgccggtc
    11641 gcaccgccag tactccgcct cacgttcgtg acgcccgccc gtcggcccgt ggtatggctc
    11701 cccgtcgtat cagtccgccg tcgttgccca agaacagcgc accgatgtcc ggatgggcgt
    11761 cgacaaccgc gagcgccgcc tccagcggga gggcggaaag gccggtcgac agggcgtcgg
    11821 cggtggtggc atcgggcgcg atcaccgaca cgctgcgata gtagttcgcg ctgccgccac
    11881 tgcgcggatc gaacaggtgg tggtgacggc cgcccacgtc gaaggccgtg ccgtagcccc
    11941 cggaagtggc gatcgcccga gccaccagag gcagcgtcag gaagctgtgc cacggccggt
    12001 cgggatccgc gatgccgatc cgccaaggtg tgccgtcggc atgaccccgc aacgcccgca
    12061 tctcccccat gttcaccagc acgtgctcga agccgtgcgc gtgcagcgtt tcggcaacgc
    12121 gatccgtgat gaagccttgc gcaaccccgt tgagcgtgat cgccgcgccg ttgcgaccga
    12181 gcgcgatgcg ctcggggctg acctgtatct gccggtaatc cactcgggag agcgcttgct
    12241 cgacgtcagc tgcggtgggt ccgtccacgt cggcccggtt ggcggcgaag tggcggacga
    12301 acagttccca caggggttgg acggtgacat cgaacgcgcc tgaagtggcg cggccgaagt
    12361 cgtgagcccg ttcgaggagc gccaccagtt ccgccggcgg atgttccaac cggccgttgc
    12421 ggttgagttg cgacagaatg gaggccggtt gctcgaggct gaatatgttc tccagtcggc
    12481 gcacttcggc gaaacaccgg gcgatggccg cgcgcgcggc ttgcgcgtcg ggatgataga
    12541 gctggatttc ggtgtcggcc ccgagcagga agtcgcgctg gacatggcgc atgccagccg
    12601 aggtcgctgg gcgggtcggc aacaccgcgc tgccgaccgc ggcggctgcg atcttcagga
    12661 agcgccgccg gcccgagcgg tcggccgcag ggtgcctgcg gttcaccggg ccgactcccg
    12721 gactctcgca tccaggagca acgccacgcg cgccaggcgc gtcagcgccc gcacggacag
    12781 cgtggccccg gagatgccgt cgatgttgcg atcgagctga tgtgtggccg acagtgtggc
    12841 gcccttgaac tgggccgtga agaagggata gcgcacctcc cagccgtggc tctctcgata
    12901 gatgagcacc gcgatgcgcg cgatcgcacc ctgatcgacc acgatgccgg tggtgatcgg
    12961 ctgggtcttg ccgatctcct cgaggatcca cgcggtgcgc gcaccccgcg cccaatagcg
    13021 cacgcgcacg gcacccgggc tgtggcccag gatcttatgc acggcatcgc gcgtctcacc
    13081 gctgatccat aggacatccg gcttcggcac gttgccgtcg aagctctcgg cgagaaacgt
    13141 ttcggccggc agatacacct cggcggccat cgcccgagca ggcagcagcg cgtccggtga
    13201 cagcagcgac aacagcgccg cggtgaacag gaccagtgtt gtcccgcgaa tcatgctctg
    13261 caggcagagg gaaaccgcct cgcggccatc ccccctgccg tcctcaaaac atgaacccga
    13321 cgccgagatt cacccggtcg tcgtcgacca tgcccgccgg tacgctctgg aactggtaat
    13381 cggccttgag caccacctgc gggtgcggcc aatagttcag ccccacggtg gtttccttga
    13441 gcttggagtc ggtcgtgtcg cccgcggtat tgtcggtttc cgcgtaacgc gcgaacaagc
    13501 ccagatcacc aaagcgggtg ctgaagatgt atcccggctc tacataccac ccgtactgtt
    13561 cgtcgcggcc gatcgcctcg ggctgatcgc cgtcgagatc ccagcgggcg tacagcgccc
    13621 gcagccacca ggggccgcgg cggattgccg cgtgagtttc gagcagcacc gcgtggacgt
    13681 cctgagcccc ctgagtgagg tcgtcctgat actgcgcgct caccgccagc tcgacgcccg
    13741 gtacgccggt ccacttcagc cgcccggtca ccgcgggatc ctctgcgggc gcctcggcca
    13801 ccttttggcg cccgccgcgg atcagatatg cgccgctgcc cgtggtgctc acctcgagcc
    13861 cggagtggta agcgacgtcg gcactgaatc cgcgcccgag gatggcacgg ccggcgacac
    13921 cgccctccca ccaagtggtg ggaatgatgt tggtctcgac gatgttgcgc tccacgccga
    13981 agaaggtcgg cggctcgtgc gtttcgttga ggatgccgat ggggaggagg aacaggccga
    14041 ccttagcctg cgtgcgctcg ctgaagtcga actcgacgta agcctgctcc agctcgacct
    14101 ccccgggcag gtcgtcgccc gccagggagt gctccagttc cacctccgag aagaaacgga
    14161 tgcggtcgct gaactgatga ccgaagaaga gcacgaaacg gtggaaatcg atctcgtcgc
    14221 gcaggccacc gttgtagtgc agctcgccat agccgccgat ggtggtcttg ttccaccacc
    14281 cttcgaccat gccgcccttg cccgggtcct tggcggcgac ctcctcgacg cgctcgccgg
    14341 tcgcctcgac cttctcatcc gtctggtcga gttgcgtttt caaccgctcg atctcgcgct
    14401 tctgctcctc catcgcgcgc tgttgctcct ggatgacgcg ccacatctcc tcttggctgg
    14461 ggggttggtc ctgagcggat gcggccccgg cgacgagcac cccgaccgcc agatggatcc
    14521 aggacaaccc ctgccctttc ttgctcttca tcgttttccc ccttcctttt agtagcttgc
    14581 gtacccgtcc ggatgggtcc accaggcatc ccatcggtgg ctgggatgca tgtcgatgga
    14641 gccttcgggc agggcatgcg aatcataatg cgagctattc gcatttcgca agcaggctcg
    14701 agcgagcccg ggtttggagc agagcgagtc agcgcccgcc agggtgcgga ttggggctgc
    14761 gccgtggggc gctcgctgcg ctttcgcccg cgacagcgga aaagcgtcag cgcccttcgg
    14821 acaacgcctg cagcagcttg gtgtgtatgc cgccgaagcc gccgttgctc atcaccagga
    14881 cgtgatcctc ggcgcgcgcg tgcgccacga tggcctgcac cagccggtcc aggtcgtcgt
    14941 ggcaggcgac cttggcgccc agcgtgcgca gcgccccggc cgcatcccag ccgatgcggt
    15001 tggtgtagca gaacaccagg tcggcgccgc gcaggctggg tgccaggctg tccttcatcg
    15061 cccccatctt catggtgttg gagcgcggtt ccagcaccgc gaggatgcgg cgctcgccga
    15121 tcctggcgcg caggccggcg atggtggtct cgatggcggt ggggtggtgg gcgaaatcgt
    15181 cgtacaccgt gatgccgttc accgcgccgc gcacctcgag cctgcgcttg acgttgcgga
    15241 agcggccgag cccctcgatg cccaccggca ccggcacgcc ggcatggcgc gcggcggcga
    15301 gcgcggcgag cgcattgctg cggttgtggg cgcccagcag gtcccagcgc accacgccct
    15361 gcggttggcc gcgcagtcgc acctcgaagc taccgtcggc ggccggaaca gcgttccagc
    15421 cctcctcgac cccgaggtac tcgaccggtg tccagcagcc gcgcgcgagc acgcgccgca
    15481 ggctttcctc gcgtccgtcg gccacgatca ggccgttgcc ggggatgatg cgcaccaggt
    15541 ggtggaactg cgtttcgatg gccgccaggt cggggaagat gtccgcgtgg tcgtactcga
    15601 ggttgttcag gatcgcggtg cgcgcgcggt aatggacgaa cttgctacgc ttgtcgaaga
    15661 acgcggtgtc gtactcgtcg gcctcgatca cgaagaacgg tgagtcggta gcgcgcgccg
    15721 agacctcgaa atccaccggt acgccgccga tcaggaagcc cggcgtgaga cccgcatgct
    15781 ccagcaccca ggcgagcatg gcgctggtgg tggtcttgcc gtgcgtgccg gccaccgcca
    15841 gcacccactt gtcgcgcaac acgttctcgt gcagccactg cggcccggag acgtagggca
    15901 gattgcgccc gaggatcgcc tcgaccaggg cgttgccgcg cgagaccacg ttgccgatga
    15961 cgaacaggtc gggcttgaga tcgagctgct ttggatccca accctcgatc aacgcgatgc
    16021 cctgcgcttg cagctgggtg ctcatcggcg ggtagacgtt ggcgtcgcaa ccgctcacgc
    16081 ggtgccccgc ctgcttggcg atcaccgcga tgccgcccat gaaggtgccg cagatgccga
    16141 ggatgtggat gtgcatgctc aaccgaagaa atgcgcgagc gcgttggccg cgatgcgccc
    16201 gccccactcg ggcacaaagt gcccggcgtc ggccacctcc atgggcgccg ggcaggcgcg
    16261 gatgtgcgcg tgcagggcgc gcatgaccgg cgggccgagc accggatcgg tcatgccgat
    16321 gcccatgaag ctcacgccgt gccagtcgcg gctccaccag tcgcgtgccc ggcgcgacag
    16381 ctccgcgccg ggggcatcga gccgctcggg cacgagctgc ggaaagcgcc gcacgcccgc
    16441 cttgtagcgc gcatcgggga acggcgcatc gtaggcggcg gcttcctgcg gcgtgaggtg
    16501 cgggcaggcg cgcgccagca gcttgccggc ggacaggtcg gggttgttcg ccacccaggc
    16561 gcgccacgcg gtgaaacccg ccgacagcgg tgcatcgccg gtgccgagcg ccgtgttcat
    16621 gaccagcaac cgcgtgaagc gccccggcag atcgagcggc agggtcaggc cgagcagccc
    16681 gccccagtcc tggcacacca gcgcgatgtc gcgcagatcc agcgcgcgca ccagcgccag
    16741 cagcgcctcg cggtggaatt cgaaggtgta ggcgccctcg tcctgcggct tgtcggaccg
    16801 gccgaagccg aacaggtcgg gcgccaccac gcgctgcccg gcctgcacga acaccgggat
    16861 catgcgccgg tacagatagg cccaggtcgg ctcgccgtgc aggcacagga acaccggcgc
    16921 gtcgcgcgga ccttcgtcca cgtagtgcat gcgcaacccc tggtacccgg gcagcgtctc
    16981 gaggtagtgc gacgggtagg ggaaatcggg gagcgctgcg aaacgctcct cggcggtgcg
    17041 caacgcgggg aagacggaca tggcggcgca attctacagg aggcggccga gatctccgcg
    17101 cgcgaaaccc gccaaccgcg aacccatccc gcggcccagg gcgatggcct tgaacgactc
    17161 gcccatctcg gacgggctca gcagccgctg cgcctgcgcg gcgagcggga gatagcgccc
    17221 cgcctgctcc gccggtgtgc gcgccagcag ctcggtgatg cccaggttga tcaggaagtg
    17281 ggactgggtg gtgtaaccga ggagcgtgag ccccgcctcg accccggcac gcgccaccgc
    17341 gctgaagttc acgtggcagg tgatgtcctg cagaccgggg acgaagaacg gatcgtcgtg
    17401 ggcccgatgc cggtaatgac acatgagcgt gccgcgcgcg cgttgcgggt ggtaatactc
    17461 ggcctcgccg aagccgtagt cgatgaacag cagcacaccc tgcgcgagcc gctccgccag
    17521 cgagcgcacc agcgccgggg ccgccagcgc gatctcgctg gcatagggga ccggcggatc
    17581 gacgcgctgc gtctgcgcgc gcagcgcacc atcgggcagc gcacgatctt cgaactggaa
    17641 gcggccctca gaccatgtca cgccgcgctc gaagatgtcg tcctcgcgcc agacgatcag
    17701 gtgcacgggc agcgcatcga gcacctcgtt ggcgagcacc acaccgcaca gcgcctcggg
    17761 cagccgctcg agccattcga cgcggcgcgc cagagggccg ggcaactgcg ccatgcgctc
    17821 ctgttgccga gcccgcaggt cggcgctcgg ctccaggatg cggtagcgcg cgggcagccg
    17881 gtccaggtcg tccagcgccg gcagcaggtc tgcggccagg cggccggaac ccggacccag
    17941 ctcgagtacg tctcccggcg cttgcgccag gatctccgcc acctgccggg cgagcgtcgc
    18001 cgcgaacagc ggcgacaact ccggtgcggt gacgaaatcg ccctcgccgc cgaacttgga
    18061 cgcaccgccg acgtagtagc cctgcccggg cgcgtacagg gcgcgttcca tgtagcgcgc
    18121 gaagtcgatc cacccgcccg cggcctgtat gtcgcgcgcg atgctgcgcg tcagcgcctg
    18181 gctgtgggcc agggcctcgg gcgggggcgg gcggggatcg cgcgggagca aggcgggagc
    18241 gagggtcggt tgcggtagaa ttctacgcag gaagcgggag agcacggcga tggctgagaa
    18301 ggtcgcgctg atcacgggcg gcgcgcgccg ggtgggcgcg gcgatctgcc gcctgctgca
    18361 tgccagcggc gtggacctga tggtgcacta ccgtcgatcg gcggacgcgg cgcgcgcgct
    18421 gcagagcgag ctgaacgccg cgcgcgccga ttccgtggcg ctggtgcagg ccgacctgct
    18481 caacctgtcg tcgctgccca cgatggtggc ggagacggtg cgccagttcg ggcgcttgga
    18541 cgtgctgatc aacaacgcct ccagcttctt ccccacgccg gtgggggaca tcacggagca
    18601 ggagtgggac gacctggtcg gcaccaactt gaagacgccg atgttcctgt cgcaggccgc
    18661 cgcaccgcac ctcaggcgca accagggctg tatcgtgaat atcgtggaca tccatgccga
    18721 ccgaccgatg cgcaaccacg tggtgtacag cctggccaag ggcgggctgg tggcgctcac
    18781 gcgctcgctc gcttccgagc tcggcccgga ggtgcgcgtg aacggcgtgt cgccgggggt
    18841 gatcatgtgg cccgaagacg accgctgggc cgacgagctg gcgcgccagc gcatcgtgca
    18901 gtccaccctg ctcaagcgcg tgggcgatcc cgaagacgtc gccaggacgg tgcgcttcct
    18961 ggtgttcgac gccccctaca tcaccggcca gatcatcgcg gtggacggcg ggcgcagcat
    19021 tcatctgtga tccgcgggca atcatggacc agcaacgccg acactacgaa gccaacaagc
    19081 tgcagaagcg cctgcgacgt ctcgccggac aggccatcgc cgacttcgac atgatccgcg
    19141 gcggcgaccg cgtcatggtg tgcctgtcgg gcggcaagga cagctatgcg ctgctcgacc
    19201 tgttgttgag cctgcaggcg cacgcgccgg cgcgcttcga ggtgttcgcc ttccacctcg
    19261 accagaagca ccccggctat ccggcgcatg tcctgcccga ctacctgacc gcgcgcggcg
    19321 tgccgttccg ggtggtcacg caggacacct acagcgtggt caagcgcgtg ctgccggaag
    19381 gcgccacgct gtgcggcctg tgttcgcggc tgcgccgcgg cgtcatctac cgggtggcgg
    19441 aggagatggg cgccacccgg atcgcgctcg gccaccatcg cgacgacatg ctggagacct
    19501 tctttctcaa cctgttcttc gccggccggc tgaagtccat gccgcccaaa ctggtgagcg
    19561 acgacggccg gcacgtcgtc atccggccgc tcgcctacgt gcgcgagcag gacctggagc
    19621 gctacgcgcg catcaggggc ttcccgctcg tgccctgcga cctgtgcggc tcgcaggaca
    19681 acctgcagcg ccagcaggtg aagcagatgc tgcgcgactg ggacaagcgt ttccccggcc
    19741 gcgtccagtc catgttccgc agcctgcgca acgtcgtccc gtcccatctc gccgacaccc
    19801 ggctgttcga cttcgccggc ctgcagccgc tggggacacc ggccgtaggc ggcgacctcg
    19861 cgttcgacga ggatcccttg tcggtttcct gatcaacctg aacgcatgac catcgtcaat
    19921 ttcacgccgt tttccgcgct gctcggcggt gtcctgatcg ggctggccgc tgccctgatg
    19981 tggtggttgc tcggccgcgt ggcgggcgtc agcaatatcc tgggccaggc cttcagcagc
    20041 gacgagcgcg agttgaactg gcgcctcgcc ttcatcgccg gcctggtgct ggcgggcctg
    20101 ggcgccaagc tgttcgtgcc cggtgcggtg cacttctcgc tgccggccgg ctacggacag
    20161 ctgatcgtgg ccggactgct ggtcggcttc ggcacgcagc tcggcggcgg ctgcaccagc
    20221 ggtcatggcg tgtgcggcat gtcgcggctg tcgccgcgct cgatcgtcgc gacgctggtc
    20281 ttcatggccg ccggcgcgtt cgccgtgctc gggctgcgcc tggcgggtac gctatgaagc
    20341 gggtcatcgc ctcattcctg gccggcctgc tgttcggcgc cggcctgatc gtcgcgcaga
    20401 tgaccaaccc gtccaaggtc atcggttttc tggacattac cggcaactgg gacccgagcc
    20461 tcgccttcgt gatggtcggc gccatcgggg tgtatggcct ggcctactgg ctatcgcgcg
    20521 cgcgccaggc gcggccgttc ctggtcgacg ggttcaattt gcccatgcgc cgtcagatcg
    20581 accggtcgct ggtcctgggt gcgctgatct tcggcgccgg atgggggctg tcgggtttct
    20641 gcccggggcc ggcgctcacc agcgccggct tcggtgagcc gcgagtctgg gtgttcatcg
    20701 ctgcgatgct ggcggggatg atgatgtatc gattgcgcgg gcgcgccgcg cgcgccgccc
    20761 ccgccaccgg cgattgaccg ccaggcggcg cgcccctcag gggtacgcgt acttcaggtc
    20821 ggggaacaac cccaccatga tgccgtcgac ctcggccgac agttgtccgt ccaccacggc
    20881 gacgccttcg gcgaatgcga gcgggttggg gcgttcggcg atccgacgaa agtcgatccg
    20941 gtaggaaacc agcttgtggt gcggccggac ctgttcccgg aacaagatct tgccgacctt
    21001 cagcgcacga ccgtagccac cgaacccggc atgatgcagg aagaagccgc acagctgcag
    21061 gatggcgtcc acctcgagga tgccgggcat cacgggatcg ctttcgaagt gggcgaagaa
    21121 gaaccattcg tcgaagcgaa cgtccttctc ggcgaccagc cagccggcat cgtgcttgcc
    21181 gccgtcggta tggatctcgg ggatgcgatc gaacatcagc agcggaggcg ccggcaggcg
    21241 ccgcggcaaa gtcgggtgat gcacgcagaa cgcgaggatc tcgcgcttgt cgtaggcgat
    21301 cttggggttg gccttcacgc cttgcgaccc gcttccagcg cggcgaggcg ggtcttgagc
    21361 tccctcagct ccgctctcag gtcggcgagg cggcgtgcca cggcctggtt cttcatgtag
    21421 tccttgagcg gttgaaccgg gctgccggcg taggtgcccg gcgccctgat gtcgttggcc
    21481 acaccggccc ggtgcaccag cacgacgtcg tcgcagagcg tgagatgatc gaggatgccg
    21541 gtcatgccgc tggcgatcac gcgcctgccg agtgtggtcg atccggcgac gcagaacatc
    21601 gccgtgagga tgcagtcctc accgatccgc acgttgtgcg cgatgtgaca caggttgtcg
    21661 aggatcgtgc ccgcgccgat gcgggtttcc tcgaacgccg cgcggtcgat gcagcacgcc
    21721 gcgccgacca cgacatcgtc ctcgaccacc acgcggccca gctgcggcac gcggtggttg
    21781 cggcgcatct cgtcttgcgc gaatccgaac ccctcggaac cgatcaccgc tcccgccttg
    21841 atgatgcaac gccggccgat ctcgcagcgc cagccgacga ccgcgttcgg atgaactacg
    21901 ctgtcctcgc cgaggacggc gtcgcgctcg atcaccgcgc cggcgagcac cacgcagcgc
    21961 tcgccgacgc gagcgccggc gccgacgatc gcgccggggc cgatgccggc cgatgcggcg
    22021 acaaccgcgg tttcgtgcac cacggcgctc ggatgcacgc gcggccatcc ttcgctgcgc
    22081 aggtcgcggt cgaagtagcg ccgcaggatg tgcgcctgcg ccaggcgcac gttgcgcgcc
    22141 gtcagcacgg cgaggcccgg caggcgcgcc gccaagtcgg gccgggtgac ggcgaccgcg
    22201 ggcttgcgtt gccggatggt ctcgagatag acctcggcgt cggcgaacac caggtcgccc
    22261 ggaccgcatg tttctaccgc ggcgacgcct tcgatcatcg cgtccggtcc ggtgcgatcg
    22321 accaggagac cggtcccctt gaggtcgtcg agcagctggg ccgcggtcaa agcaggcatg
    22381 gtgcagcagt gtacccgatg tgcacgccga atccggctgt cctccgcccg atcggtcagc
    22441 ggatgagtcc cccttttccg cgcatgcgcg tcgctgctgc agcgtatact gtggcgggcg
    22501 agactggaga tcaacgcgca caagggaggc gtaatggatc cagtgagagt ggccaggatg
    22561 gacgcgtaca ccatggtgtc cgggatggac aaggccgccg tcgagcgcaa agtcgccgag
    22621 ctggtgcgcc agggcgcacg tgcggtgacc aaggtcacgc cgctggggcg caactgggtg
    22681 gccacactcg aagacccgac cgcccgcccc gatccggacg gctgccgcgt cgcccgcttg
    22741 ggcctgcaga tcatcgtcac cggaccggac aagaccagcg tgcagaagcg cgcggaggcg
    22801 ctgatgaaag agggcgctgc cctcaagtcg ccgcctcagg aaaacaccaa cggctggtcc
    22861 gccgtgctcg acgaggccgg catggaccga ggcgcctgat gcgcccttaa ggcgcgtctt
    22921 actgtaagaa gcaactgcgc gcggcgcgcg cgtggacttg taggccgcct cgggacccgc
    22981 aagctttttg ctttttgaga cgcgccttaa gggcgcatca ggcctacaac ccgaggcgct
    23041 gccagatggt ggtagtgggg ccggcctgat tgagggtata gaagtgcagg ccgggcgcgc
    23101 cggcgcgcag caggcgatcg cacaggctgg tcaccacgtc caggccgaaa gagcggatcg
    23161 aggcggcgtc gtcgccgaag ccttccagct tgcggcggat ccagcgcgga atctccgcgc
    23221 cgcaggcgtc ggaaaagcgc gcgagctgcg caaaacgatt gatcggcatg atccccggga
    23281 cgatgggcac ggtgatgccg gcggcttcgc actcctcgac gaacgcgaag taggcgtcgg
    23341 gattgtagaa gtactgggtg atggcgccgt ccgctcccgc ctcgaccttg cgcttgaagc
    23401 tgagcagatc gccctgcgca gtgcgcgcct gcggatggta ctcggggtag gccgccacct
    23461 cgatgtggaa ggtgtcgccg aactccttgc gcacgaacgc gaccaggtcg ctggcgtagc
    23521 ggaactcgcc ggcgtcggcc attcccgacg gcagatcgcc gcgcagcgcg acgatgcggc
    23581 gcacccccgc cgcatggtag cgctccagga tctcgcgcac cgaggcggcg ctggaagcca
    23641 cgcaggagat gtgcggcgcc acctgctggc cctcggcctg gatctcgagc gccacgccga
    23701 gggtgcgctc gcgggtggtg ccgcccgcgc cataggtcac cgagaagaac gccggcttga
    23761 gctgagcgag ctgccggcgc gtgacgcgca gcttctccag cccctccggc gtctgcggag
    23821 ggaacagctc gaaggagaag accttggcgc ccactcggaa cgccggccgc agatcgcggc
    23881 cggccggacg atcaataacg atagtgatcg ggcttgtagg gcccttcctt gtgcacgccg
    23941 atatagcgcg cctgcttgtc ggtgagctcg gtcagcatcg cgcccagctt cttcaactgc
    24001 aaccgcgcga ccttctcgtc gagatgcttg ggcagcacgt acacgcccac cgggtatttc
    24061 tccggattgg cccaaagctc gatctgcgcc atcacctggt tggtgaacga cgacgacatc
    24121 acgtaactcg ggtggccggt ggcgcagccc aggttcacca ggcggccctc ggccagcagg
    24181 atgatgcgct tgccgtcggg gaagatgatg tggtccacct gcggcttgat gttttcccac
    24241 tggtactgcc tgagggaggc cacgtcgatc tcgttgtcga agtgcccgat gttgcacacg
    24301 atggcgttgt gcttcatgcg ccgcatgtgc tcgtgcgcga tgacgtcgat gttgccggtt
    24361 gcgctgacga agatatccgc cttgtcggcg gcgtaatcca tggtcaccac gcgatagcct
    24421 tccatcgccg cctgcagcgc gcagatcgga tccacctcgg ccacccacac ctgcgcggac
    24481 agcgcccgca gcgcttgcgc gctgcctttg ccgacgtcgc cgtagccgca caccagcgcg
    24541 atcttgccgg ccaccattac gtcggtggcg cgcttgatgc cgtccaccag cgattcgcgg
    24601 cagccgtaca gattgtcgaa cttggacttg gtcaccgagt cgttgacgtt gatcgccggg
    24661 aaccccagct cgccgtcctt gtgcatctgg tacaggcgct tgacaccggt ggtggtctcc
    24721 tcggtcaccc ccttgatgcc ggccttgagc ttcgagtaga acttggagtc ctggtccagc
    24781 cggcgccgga tcgcagcgaa cagggcgcgc tcctcgtcgt tggccggacg gccgatgcaa
    24841 gccggctcgg tctcggcgcg cgtgcccagg tgcaccagca gcgtggcgtc gccgccgtcg
    24901 tcgagaatca tgttggcgcc gcggcccgcg ccgaaggcga agatgcggtg cgtgaactcc
    24961 cagtactcgt ccagcgactc gcccttgtag gcgaacaccg gggtgcccgc cgccgcgatc
    25021 gcggccgccg cgtggtcctg ggtcgagaag atattgcacg acgcccaccg cacttcggcg
    25081 cccagggcct gcagcgtctc gatcagcacc gccgtctgga tggtcatgtg caggctgccg
    25141 gcgatgcgcg cgccgcgcaa cggctgctcc ggggcgaact cctcgcggat cgccatcagc
    25201 ccgggcatct cggtctcggc gatgcggatc tccttgcggc cccagtcggc cagggccaga
    25261 tccgccacgt ggaaatcggt ggcttgcttg gattgcagga ctgcgctcat gttgcctccg
    25321 ttcgacgatt cggaacgggc gccgttcgct tgccgacccc ttccgagcct ggcaggagcc
    25381 cctgtcgcag cgcccctcgg agcgggtccc aaaaaaaacg ctcgcacccg attaggggtg
    25441 cgagcgccgt tggtatcgct gagcctggcc ggtcttcaga ccggtcgcag cgctcctcag
    25501 tcgacccgta ttataccgct tcccgcttgc ggcaggaagc gctcaagcgt gcagccgtac
    25561 tccggcctcc gcgcgcagcg cctcgacctt gtccgaccgc tcccaggtga actccggctc
    25621 ttcccggccg aaatgcccgt aggcggcggt cttggcgtag atcgggcgca acaggtcgag
    25681 catctggatg atgccccggg gccgcaagtc gaagtgctgc tgcaccagtt gctccagctt
    25741 ctggtcggcg accttgccgg tcccgaacgt ggtcaccatg atgctggtcg gtcgcgccac
    25801 gccgatggcg taggacacct ggatctcgca tttgctggcc aggccggcgg ccacgatgtt
    25861 cttggccacg tagcgcgcgg cgtaggccgc ggagcggtcc accttcgagg ggtccttccc
    25921 ggagaaggcg cccccgccgt ggtgtgccgc cccgccgtag gtgtcgacga tgatcttgcg
    25981 gccggtcaga ccgcagtcgc cctgcggtcc gcccaccacg aatcgcccgg taggattgac
    26041 cagatacttg atttcgccct tcatcaagtg cctgggcagg accggtttga cgatctcctc
    26101 gatcacgctc tcgcgcacct tctccagcgt gacgtccggc gcatgctgag tggacagcac
    26161 caccgtgtcg atactgtccg ctttgccgtc gcgatagcgc accgtcacct gcgacttcgc
    26221 gtccggccgc agccagggga gccgcccgtc ccggcgcaga ttggactggc gctccaccag
    26281 gcggtgcgcc agatagatcg gcagcggcat cagctgcggc gtctcgtcgc aggcgtagcc
    26341 aaacatcagg ccctgatcgc cggcgccctg attcagcggg tcgtcctgcg cctggtccac
    26401 cccctgcttg atgtccgggc tctgcttgtc gtaggccacc agcacggcgc agcccttgta
    26461 gtcgatgccg tactcggtgt tgtcgtagcc gatgcgccgg atggtctcgc gcgccacatg
    26521 ctggaaatcc accacggcgc tggtggtgat ctcgccggcc atcaccacca gcccggtgtt
    26581 gaccagcgtt tccgcggcca cccgtgcggt tttgtcctgg gctaagatag cgtccagcac
    26641 ggcgtcggag atctgatccg ccaccttgtc cggatgcccc tcggacaccg actcggaagt
    26701 aaataggtat tcgctcatta cgaaagccgc tcgcgcgcgt tttggaaacg ggccaaagaa
    26761 tagcagattg cgcctgcggc acaaaggctg agggcgagct ctgacattct tgttgcctcc
    26821 ggcggtccgg tcgccgccgg ccgggttcca cgcgctgcaa gcgctcctcg ggcgcatcgc
    26881 ccacgtcgct tccccatgct gctcgtcgag gctgcgcgcg aacgaggtgt gcgccgcgca
    26941 ggtccgcacg cggcacaatt cacgcgcgcg ttttcacatt tcgcttcccg cgctgtccgc
    27001 atgagccgcc tcggcatcct gctgctgtgg ctgctgcact tcctgccgct ggcgctgctc
    27061 gcgcccatcg gccgcgggat cgggctgctc gcctacctgc tgatcggcaa gcgccggcgc
    27121 atcgcccgca ccaacctgcg gctgtgcttt cccgagttga gcgatcggca gcgcgagcgc
    27181 ctgctgcgcg cgcatttcca gtgcttcggc cgcgccctgg tggagtccgg gatcgcctgg
    27241 tgggggtcgc cgcaacggct gcggcgcgtg gtgcgcatcg aaggccagga tcaggtggag
    27301 cgtctgcggg gcgagcgctt catcgctctg gtgccgcatt tcgtgggcat cgagctggag
    27361 ggcttgcgca tggccatgga ctaccgcggc gtggccgtgt acgtgcgcca gaaggacccg
    27421 ttcgtcgatg cattcctcaa gcgcaagcgc gagcgcttcg cgggcacgcg catgatcctg
    27481 cggcgcgaag gcgtcaaggc catcttgcgc gccctgcagg gcggccaggc gctgcagctg
    27541 tcgccggaca tggacctggg gcctaaggac gcggtgttcg tgcccttctt cggcgtcccc
    27601 gccgccaccg tgaccgtgct ctcgcgcatg gcgcggctca tgaagctgcg gatcgtgccg
    27661 ctggtggtgc gccagcttcc cggcgggcgc ggctacgtgg cgcgcttcta tccggcgtgg
    27721 gaggattacc ccaccgcgtc ggtcgaggac gatacgcgcc acatgaacgc cttcctcgag
    27781 gagcgcatcc gcgagatgcc ggagcaatac ctgtggacgc accgccgctt caagacccgc
    27841 cccaccggcg agcgcggcgt gtattgagac cgcattgcgc attcgtccag catcatcggt
    27901 ttcgagcgga tagaataggt gccggtgctg ctgtccaatg ataccggtgc tgctgtccaa
    27961 tgataccggt gctgttgtcc aactgatacg ggcgccgttg tccgatgata tcggcccagg
    28021 tcgatgcggc tgaagttcac caagatgcag ggtgtgggca acgacttcgt ggtgatcgac
    28081 gcggtcaacc agcgggtgcg cctggacgcg cccgccgcgc gccggctcgc cgaccgtcac
    28141 ttcggcgtcg gctgcgacca gatcctgctg atcgaggcgc cgcgcagggc ggatacggac
    28201 ttctactacc gcatcttcaa ctccgacggc ggcgaggtgc agcactgcgg caacggtgcg
    28261 cgctgcttcc tgcgctacgt gcgcgacaag gggctgacga gcaagagtgc gatccgggtg
    28321 gaaacgctgt ccggcgtgat cgtgccgcgg ctggagcccg acggccaggt gacggtggac
    28381 atgggcccgc cgcagttcga gcccgcccgc atcccctttc tcgccgagcg ccaggcgctg
    28441 acctactggc tcgaggtgaa cggccagccg gttgagatca gcgcattgtc cgtgggcaac
    28501 ccgcacgccg tgcagatcgt ggaggatttg caggcggcgc ccgtgctcac gcaggggccg
    28561 ctgatcgagc gccacccgcg cttcccggag ggagccaatg ccgggtacat gcaggtcgta
    28621 gaccgcggtc acatccggct gcgcgtatac gagcgcggcg cgggcgaaac gctggcgtgc
    28681 ggcaccggcg cctgtgcggc ggtggtggcc ggcatcggcc gcggccatct ggacgggcgc
    28741 gtgcgggtga ccacgcacgg cggagacttg accatcgcct ggaccggcga gcggcagccg
    28801 gtgctgatga ccgggccggc cgagagcgtt ttcgacgggg agatcgagct ttgaccatcg
    28861 gcacactgcg gcccgaggag gtcgccaagt acctcaagga ccatccggag ttcttcgagc
    28921 agtacgcgga gctgctctcc ggcatctacc tgccgcatcc ctacggcggc cacgccatcc
    28981 ccctggccga acgccaggtg ctcggtctgc gcgagcgcag ccgggcgctg gagggcaagc
    29041 tgcgcgagct ggttcagttc ggcgaggaga acgacaccat cagcgatcgc gtgcaccgga
    29101 tcagcctggc gctgctcgcc gcgcccgacc tggccggcgt gctgcaggtg ctcgacaccg
    29161 gcctgcgcga ggatttcgca gtgcccgaag tggcgctgcg gctgtggcct ggacagtcgg
    29221 acgccgaccg ccccgagctg gagccggtaa gcgaagaggc gcgcgtgttc gccgacagcc
    29281 tggccggtcc gtatttcacc gaccgcccga tgttcgagag cgcggaatgg ttcggcggcg
    29341 gcgaggtgct gcgctcgttc gcttacgtcg cgctgcgcgc ggaacgaccc ctgggcgtgc
    29401 tggcgatggc gagcccagag cccgaccgct tcacctccga catgggcacg ctgtacgtag
    29461 cgcgcctggg cgagctggcc ggcacggccg tgcgccgcca tcttccggac tagcccggcg
    29521 cgatggccga ccgggcttcc gcctcgcagc gcctgctcga cgcctttctc gcccatctcg
    29581 cgcaggaacg ccggctgagc ccgcacacgg tgcgggccta cgctcgcgac gtgcgcgttc
    29641 tgctcgacct caccgcggat gccgtcccgc tggccgacat ccgcatgcat caggcgcgcc
    29701 ggctggcggg gcagctgcat gcgcgcgggc tcgacggccg atccatcgcg cgcatgctct
    29761 cggcgtggcg cgccttgttc cgctacctgg cgcgcgatca cggcttcgct cacaacccgt
    29821 tccagggaat ccgcgcgccc aggtcggcga agaacctgcc caaggcgctg tcaccggacg
    29881 aggccggcaa gctgatgcag ctccccgcgg tcgacgatct cgcagtgcgc gatcgcgcga
    29941 tctgcgagct gttctattcc tccggcttgc ggctgtccga gctgaccggg ttgcgcctgg
    30001 gcgatgtgag cttcgacgat cagaccgtgc gcgtcaccgg caaaggcagc aagacgcgcg
    30061 tggtgccggt cggacgcttc gccctgcagg cgctgaccgc ctggctgccg gcgcgcacgg
    30121 cgatgacgcg gcccgaggag cgggcgctgt tcgtcgcccg caccggccgg ccgctggccg
    30181 cgcgcggggt gcagcgctgc ctgcggcgcc gcgccgtgca gcaggggctg acgcagccgg
    30241 tgcacccgca catgctgcgc cactcgttcg cctcgcacct gctgtagtcg agcggcgact
    30301 tgcgcgcggt gcaggagatg ctgggtcacg ccagcatttc cacgacacag gtgtacacgc
    30361 acctggactt ccagcatctg gcgaaggtct acgacgccgc gcatcctcgc gcgcgcaaga
    30421 agaagacctg aatctgcagc gctggccgtg gcggcagacc tcattctcaa gcccggccgg
    30481 gagaagtcat tgctgcgccg gcacccgtgg gtcttctcgg gcgcggtggc gcgcgtggac
    30541 ggcgagccgg gcatgggcga gacggtggcg ctgcgcgcga gcggcggcgc atttctcgcc
    30601 tggggcgcct acagcccgcg ttcgcagatc gtggcgcggg tgtggagctg gaacgagcgg
    30661 gagccgcccg gcccggcgct gatgcgggtg ctgctggcgc gcgccatcgc catgcgcggc
    30721 gagcggctcg ccgatgcgca ggccgtgcgc ctggtgcacg gcgaatccga cggcatgccg
    30781 ggcctggtag tcgatcgcta cggcagcgtg ctcgtgctcc aggtgtcgag cgcgggtgcg
    30841 gcccggtggc gcgacgcgat cgccgacgcg ctgacagcgc tgaccggcgt gcacgacatc
    30901 tatgaacgct ccgacgccga cgttcttgcg ctggagggcc tggcgcagca ggtgggcgtg
    30961 ctgcgcggcg cggcgccgca cgatcccctc atcgtcgaag agcacgggct gaagctggcg
    31021 gtcagcgcgc tgcgcggcca caagaccggc tactacctgg accagcgcga caaccggcgg
    31081 ctggtgcgcg atctttcccc agggcgtgag gtgctggact gcttctgcta caccggcggc
    31141 tttaccctga gcgccctggc cggcggtgcg cgccgcgtga ccgccgtcga ttcttcggcc
    31201 gaggcgctgg ccgcggtgcg cgacaacgtg cagcgcaacg gcctgcacgc cgggcaagtc
    31261 gagtgtgtcg aagccgacgt gttcgaacag ctgcggcggt tgcgcaacga aagccggcgt
    31321 taccagctga tcgtcctcga cccgcccaaa ttcgcgccca cggcggcgct cgcccagcgc
    31381 gccgcgcgcg gctacaagga catcaacctg ctggcgctca agctgctcgc gcccggcggg
    31441 ctgctggtca cgttctcctg ctcgggcggc gtgtcgcgcg atctgttcca gaagatcgtc
    31501 gccggcgccg ccgccgacgc cggcaccgac gcacagatcc tgcgctggct ctcggcggcg
    31561 cccgaccatc cggtggcgct cggcttcccg gagggcgagt atctgaaggg gctgctgtgc
    31621 cgcgtcccat gaatcggaag gacatcgctt gagcgaaacg cacttcatcg tcgtgcgcca
    31681 cggggagacc gcctggaacg ccgaggcgcg cattcaagga cacctggaca gcccgctcaa
    31741 cgaggagggg ctggcgcagg cgctgctggt ggcggagcgc ctggcgcacg agcagttcga
    31801 ccagttttac tgcagcgatc tggggcgcgt gttgcagacc gtgcagccgc tcgtggaccg
    31861 caccggaaag cggccgctgc cgaccgcgcg gctgcgcgag cgcaacttgg gcgtgttcca
    31921 gggattgacc ggtgccgagt gccagcgcga ctggccgcag gactacgcac gcttccacgc
    31981 gcgcgacccg gatcacgcca tgcccggcgg ggagagtatc cgccagctgg ttgcgcgggt
    32041 gtccgctttc ttcgacgagg cggcggccgc gcacgccggc cagcgcgtgc tcgcggtgac
    32101 ccacggcggt gtgctcgacg ccctgtaccg gcacgccacg gcgatgccgc tcgatcgcct
    32161 gcgcgacttt ccgatctaca acgccagcct caactggctg cggcacagcg gcgcgggctg
    32221 gagcgtcgag cgctggggcg acatctcgca cctcacgcgc gacgcggcgc tggacgattt
    32281 ctgagccggc cctttgaact cgcccgcaaa ccgcgctaga cttcgggccg gtagaccggg
    32341 cgccgcgagc cgccgcatcg cttgtggcgc gttgccccga caggcaccac acccgctcag
    32401 catggcgcct cgacgcacca tcgcccatct gttcctggtg ctggccctgc tgctgacgca
    32461 gcaggtgctc accgcgcacc cgttcgcgca tcgtggcggc gccgccgaca cgcaggacga
    32521 ccagtccgcg ccgggcgagc ggatattgtg cgcgctgtgc gtggcaggcg cggcatcggg
    32581 cagcgccttg cccagcgccg gtcactctcc gttcgccagc gtcgcccagg tttttgagcc
    32641 gacgccgatc acctgggcct atcgcccgcc gctcgcccgc gcattctccg cgcgggcgcc
    32701 gcccatcctc ctctgagccg ttgcgcaagg ttttacgccc cgccgcgcac ggcggcggcg
    32761 tgcatcgcca attgatctca gaggagtgca ccgatgttca agctcaaggc cacggccttt
    32821 gccgtcgcgg caggctgcgc ctttccgctc tgcgcgcagg aagacgcgac catggacgaa
    32881 atccgccggg agatccggca actgaaggaa tcgtacgaga cccgcattca ggcgctggag
    32941 cagcgcctga aggacgccga ggcggcgaaa gctccgtcgc cggcagcggc ggcgcctgcc
    33001 gcggtgacgc ccgccgccaa gcaggtcacg gacaacgcct tcaacccggc gatgtcgctc
    33061 atcctccagg gcgtctacgg ggacctgaaa gagtcgccgg aggagtacgc ggtgaccggc
    33121 ttcgccgacc cggaaaagat ccatccaggc acgcgcggct tctcgctggg cgaatcggaa
    33181 ctgaacctgt cggccaacat cgatcagctg ttctacggca atctgctggt cgccttcgaa
    33241 gagggcgaag tccacgtgga ggaggcctgg ttccagactc tcgcgctcgg ccatggcctc
    33301 accgtcaaag gcgggcggtt cttctcggga atcggatacc aaaacatgtt gcatccccac
    33361 gcctgggact ttttcgacgc cgcgctggtg cagcgttcgt tcctgggcaa caactacggc
    33421 gacgacggcg tgcaagcgac ctggatcgcg ccgctgccgg tgtatctgga gctcggcgcg
    33481 gaggtcggcg ccggccgctt gctgccgggc actttcgagg agcacagcga gctggccgag
    33541 gtcgatcggg acaagaacgg tgccggcgcg tatgcgctgt tcgcccgtct cggcggcgat
    33601 atcggcacca gcaacagtta tcgcgtcggg gtgtcctacc ttgacacttc cacggacgac
    33661 cagccgttcg cgttggcgga cttcgacacg cgcaccgggg tgagcaacga gtacagcggc
    33721 gacgtgcatc tgtacggcgt ggacctggtg tggaagtggg ccccggacgg caacccggcg
    33781 cgacgcaact tcaagctggt ggccgaatgg atgcagctcg agcgcaaggg tgacctcgtc
    33841 ttcgacgttg gcggcgcagc ggtgcccgac gcgttcaagt tgcggcagtc cggctggtac
    33901 gtccagggcg tgtaccagtt catgccgacc tggcgcgtgg gcctgcgcta cgaccagctc
    33961 gatcaggggt cctccagtct cggagcgaac acgggcaaca tccccggcgt cgactacgat
    34021 cccgagcgct ggtcggcgat ggtggactgg acgccgtcgg agttcagccg ctggcgcctg
    34081 cagtacagca atgacgagtc gcgcg
//
LOCUS       DASPGE010000405         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1162419, whole genome shotgun sequence.
ACCESSION   DASPGE010000405 DASPGE010000000
VERSION     DASPGE010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162419"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="VD791_13390"
     CDS             <1..1163
                     /locus_tag="VD791_13390"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXV09021.1"
                     /translation="QQYRDVLEREGVTLHLRPSAGALENYKLLSDAHGGVDVALVQGG
                     IAGIDGGAGNVVSLGAMYFEPLWIFHRGDRALDHLGPLRGTMIAVGAEGSGTAVLAMT
                     LLHSYGIAEPPTTVLKIGDRAAANLLLSSGIDAAFFMGDAYSPLIQEMVRAPRVHLLD
                     LRRAQAYTHRHFFLTRLALPQGVFDLAGDLPPTDVAMIGTTANLFVRRDLHPALHYLL
                     LRTAAEVHSKPTLFSGVREFPAPHDPEVPLSEEAMRFYRSGSPLLQRYLPFWAANLVD
                     RLLVLLVPAAVVLLPAMRVLPILYRWRVRSRIYRWYARLKEIELELEERRTTDELQGI
                     LKRLDEIEDAVNHIDTPLAYSENLYVFRQHIDLVRLRAQARASPMSPVPFEV"
     gene            complement(1211..>2045)
                     /locus_tag="VD791_13395"
     CDS             complement(1211..>2045)
                     /locus_tag="VD791_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HXV09022.1"
                     /translation="EDEAAAVAGVGLAVRHAAQGLGAGGSALDAAVAAVVELEDNALF
                     NAGTGAVLNRDGEAELDASVMTGHDLRCGAVAALRRVRNPVLVARRVMERTAHVMLAG
                     EGALRFARGEGFEDYDPITQRARHGWLRRKAAAPGTVGAVVLDRAGGLAAATSTGGVS
                     LKLPGRVGDSPIPGAGNYAGAAAACSATGQGELMIRVLAAKELCDRVARGDHPQAAVD
                     ELLREMAAGIGADGGFILLARDGRFGVAHTMVSMPHAWWCEGEPAPSARMRVPPNRTA
                     G"
BASE COUNT          282 a          757 c          693 g          313 t
ORIGIN      
        1 cccagcagta ccgcgatgtg ctggagcgcg agggcgtcac gttacatctg cggccctcgg
       61 cgggcgcgtt ggagaactac aagctcttgt cggatgccca cggcggtgtg gatgtcgccc
      121 tcgtgcaggg cggcatcgcc ggcatcgacg gtggcgccgg caacgtggta tcgctcggcg
      181 cgatgtactt cgagccgctg tggatcttcc atcgcggcga cagagcgctc gaccacctcg
      241 gcccgctgcg cggaacgatg atcgcggtgg gcgcagaagg cagcggcacg gcggtgctcg
      301 ccatgaccct gctgcacagc tacggcatcg ccgagccccc gaccacggta ctcaagatcg
      361 gcgaccgggc ggcggccaac ctgctgctgt ccagcggtat cgacgcggcg ttcttcatgg
      421 gcgacgccta ctcgccgttg atccaggaga tggtgcgcgc accgcgcgtg catctgctcg
      481 atctgaggcg agcgcaagcc tacacccacc ggcacttctt cctgacgcgc ctcgctctgc
      541 cgcagggcgt gttcgacctg gcgggcgacc tgccgcccac cgacgtcgcg atgatcggca
      601 ccacggctaa cctgttcgtg cgccgcgacc tgcacccggc gctgcattac ctgctgctgc
      661 gcaccgccgc ggaagtccac tccaagccca cgctcttctc cggtgtgcgc gagttcccgg
      721 cgccgcacga tccggaagtg ccgctgagcg aggaggcgat gcgcttttac cgatcgggct
      781 cgccgctcct gcagcgctac ctgccgttct gggcagcgaa cctggtggat cggctgctgg
      841 tgctgctggt gcctgccgcg gtggtgctgc tgcctgcgat gcgagtgctt ccgatcctgt
      901 accggtggcg ggtgcgctcg cgcatctatc gctggtatgc gcgcctgaag gagatcgagc
      961 tggagctcga agagcgtcgc accacggacg agctgcaagg catcctcaag cgtctggacg
     1021 agatcgaaga cgcggtcaac cacatcgaca cgccgctggc gtattccgag aacctatacg
     1081 tgtttcgcca gcacatcgac ctggtgcgcc ttcgcgccca ggcgcgggcg tcgccgatgt
     1141 ctcccgtgcc gttcgaagtc tagccgcgaa cggctcggta gcccggatgg aagccaagcg
     1201 gaatccgggg tcaaccggcg gtccgattgg ggggtacgcg catgcgcgcg ctcggcgccg
     1261 gctcgccctc gcaccaccag gcgtgcggca tcgacaccat ggtgtgcgcc acgccaaacc
     1321 ggccgtcgcg cgcgagcagg atgaagccgc cgtcggcgcc gatgccggcc gccatctcgc
     1381 gcagcagctc gtcgacggcg gcctgcgggt ggtccccgcg cgccacccgg tcgcacagct
     1441 ctttggcggc cagcactcgg atcatcagct cgccctggcc ggtggccgag cacgcggcgg
     1501 cggcgccggc gtaattgccc gcgccgggga tgggcgagtc gccgacgcga ccgggcagct
     1561 tcaggctcac gcccccggtc gaggtggccg cggccagccc gcccgcccgg tccagcacca
     1621 ccgcgcccac cgtccccggc gctgcggctt tgcggcgcaa ccagccgtgg cgcgcgcgct
     1681 gcgtgatcgg gtcgtagtct tcgaagcctt cgccgcgcgc aaagcgcagc gccccttcgc
     1741 ccgccagcat cacgtgcgcg gtgcgctcca tgacgcggcg cgcgaccagt accgggttgc
     1801 gcacgcgccg caacgccgcc accgcgccgc aacgcaggtc gtgcccggtc atgacggagg
     1861 cgtccagctc cgcttcgccg tcgcggttga gcaccgcgcc agtgccggcg ttgaacagcg
     1921 cgttgtcttc gagctccacc acggccgcca ccgcggcgtc cagcgcgctg cctccggctc
     1981 cgagaccctg tgccgcgtgg cgcacagcca ggccgacccc cgccaccgcg gccgcttcgt
     2041 cctcg
//
LOCUS       DASPGE010000406         7924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1632186, whole genome shotgun sequence.
ACCESSION   DASPGE010000406 DASPGE010000000
VERSION     DASPGE010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7924
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632186"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..792)
                     /locus_tag="VD791_13400"
     CDS             complement(139..792)
                     /locus_tag="VD791_13400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MauM/NapG family ferredoxin-type protein"
                     /protein_id="HXV09023.1"
                     /translation="MADDARRLGRRKFMQDCAGLAGSAGVVGLLLTSYSRQSKALPAW
                     ALRPPGALPEDRFGAACVRCGLCVRACPFDMLHLAELGQDVATGTPYFVAREIACEMC
                     EDIPCAAACPTDALSKALTDIRDAKMGVAVLTGRDTCYAVARGANCRACYLACPIKDE
                     AITMERRHSNGRSYFEPTVHADKCTGCGKCEHDCVTAEASIKVLPVALAQHDLASAG"
     gene            complement(812..1795)
                     /locus_tag="VD791_13405"
     CDS             complement(812..1795)
                     /locus_tag="VD791_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV09024.1"
                     /translation="MFAFVLRRLAQAVAVMLTVGIIAFALFRYVGDPVVFMLGQEATP
                     EDRARLTRELGLDRPFYAQYAGFVARALHGDFGFSLRQLRPVSTLIAERLPATLELSL
                     AAALLALVVGVPMGVYTALRRTAWLSHVFLAVSLVGVSLPTFLIGILLILVFSVELGW
                     LPSFGRGETVSIGWWTTGLLTASGLKALILPSITLGLFQMTLIMRLVRSEMLEVLRTD
                     YIRFARARGLSDRAVHFGHALKNTLVPVITVTGLQLGSIIAFAIITETVFQWPGMGLL
                     FIQSVMFADVPVMAAYLCLIALFFVVINLAVDLLYYAVDPRLRIERAAAAS"
     gene            complement(1994..2437)
                     /locus_tag="VD791_13410"
     CDS             complement(1994..2437)
                     /locus_tag="VD791_13410"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HXV09025.1"
                     /translation="MKLKLVVAAAVATAAMSLSAAAQDWKLVSLDDQQLTFTDGGRVA
                     RNPVAASLWVLESYPEVRHVGEGWYPHRSRSLRYVFNCSEETYAIAQWILHQGALGHG
                     DAVWADRATAPAFAQVHAGDPQAALLAAACSGTALAQHGASGRVN"
     gene            complement(2471..3205)
                     /locus_tag="VD791_13415"
     CDS             complement(2471..3205)
                     /locus_tag="VD791_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009043159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV09026.1"
                     /translation="MKLQDKVALITGGTTGIGLAAAKLFHAEGARVFVTGRSEAGIAE
                     ARKQLPPEVEVLPSDAADLADVQALASTLKNRAGHIDVLFVNAGIARFLPIENVTPQA
                     FDEQFDVNVRGAYFTIQQILPLMSSGGSIVLTTSVAADLGMASTSVYSATKAALSSLA
                     RTLANELAPRGIRVNEVSPGPIETPIFGKIGLTAEQVDGMSALVPLKRLGTAEEVARA
                     ALFLASADSSFLLGAKVRIDGGLALN"
     gene            complement(3255..3788)
                     /locus_tag="VD791_13420"
     CDS             complement(3255..3788)
                     /locus_tag="VD791_13420"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HXV09027.1"
                     /translation="MQLFWERGYHATSIQDLVDRLGVNRQSLYDTFGGKDQLFLSALE
                     RYREIQGAPVRRLLEREGPALEVLREFLHAFADNLLSGDFKGCFIANTITELAGHDPA
                     VTQACAANARQIEGALSGLLVRAQQAGEIPADSPPLQLARFLMNTMNGLAVTAKATRD
                     PKVLNDIVEVALAAVTR"
     gene            complement(3869..4732)
                     /locus_tag="VD791_13425"
     CDS             complement(3869..4732)
                     /locus_tag="VD791_13425"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HXV09028.1"
                     /translation="MSPLVEVGLPLIIVYAMTVVGLELTPADLKRVLHYPVHVAVALL
                     GQVLLLPLLGVALILVLRPEPAVTGGLVLIAAAPQAIISNYYCLLARANIALALTLTA
                     VSSVLALVSTPIAVQIAFGLLLERQAGFSLPAGKVMEQVVIGLLLPIAAGMLVRHFAP
                     GFVERQRVRFQRLSLLSLAALIAIVLVDQGGTIARNLLPIVVAGLLFTAGALALGLAI
                     ARAFAWSRIDTVTMAAAYPSRSLSIATLIAVNVLGRPDFLSFAVVFFLVQAVLLVPAM
                     MLSRPLRAAGS"
     gene            4841..6307
                     /locus_tag="VD791_13430"
     CDS             4841..6307
                     /locus_tag="VD791_13430"
                     /inference="COORDINATES: protein motif:HMM:NF006113.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HXV09029.1"
                     /translation="MRAIWGMLLALLAALVVVLAVNTARSGSRQIEVPRADERLPELE
                     LAVQRLAQAIRLQTVSQQDPDRLPIGHFEALHRLLRDGFPRLGKALKRERIGGSSLLY
                     EWPGADPAAKPILLLAHLDVVPAEEGSLQQWAHAPFSGAVADGFIWGRGTRDDKGAVM
                     ALHEAVEALLAQGFQPRRTVYLAFGHDEEVGGTRGAAQIAQALAARGVTLESVLDEGM
                     PVTHGLLPGIQQPVALIGIAEKGYATLELVAQGEGGHSSMPPPQTAVTILGAALAKLE
                     ASRFAATLTEPLREQLAFLGPEQGLLRRVLFANLWLFGGVVKGQLANSRAGNALIRTT
                     VAPTMLEGSVKENVLPARARALVNFRLLPGTSIDALTRRVVEIVDDPRVAIAPAGTSR
                     SEPSPVSSTRSEAFRSLQRAVEAVFPEALVAPSLLVAATDSRHFAPLAESVFRFLPAR
                     TGPDDLPRYHGVNERLAVDNYAEFIGFYMRYLREAAGG"
     gene            complement(6311..7060)
                     /locus_tag="VD791_13435"
     CDS             complement(6311..7060)
                     /locus_tag="VD791_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HXV09030.1"
                     /translation="MNARDGSVATLACASDVAGRVEAIDWDRVSGDLDARGNATIERL
                     ITPAECAALAELYPDDGVFRSRVVMARHGYGRGEYKYFRYPLPGIVAGLRSPLYARLA
                     PIANRWQAAMRSRVRFPDEHAEFIRRCHAAGQRRPTPLLLQYGADDYNCLHQDLYGEH
                     FFPLQVAILLSEPGRDFSGGEFVITEQRPRMQSRPEVVPLRQGDAVVFAVHQRPVQGT
                     RGAYRVNLRHGVSRVRSGHRHTLGIIFHDAS"
     gene            complement(7057..>7924)
                     /gene="ada"
                     /locus_tag="VD791_13440"
     CDS             complement(7057..>7924)
                     /gene="ada"
                     /locus_tag="VD791_13440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HXV09031.1"
                     /translation="GFRPCKRCRPDQPALAEQHAGRVAEICRLIESAETVPSLEELAR
                     RAGLSAYHFHRVFRAATGLTPRAYAAAHRAKRVRNELRRSRTVTTAIFDAGYNSNGRF
                     YENSDQVLGMTPSRYRAGGADTQIRFAIGECSLGSILVAASERGVCAILLGDDPEALA
                     RDLQDRFPRADLIGGDRDFERIVARVVGFVEAPGLGLDLPLDVRGTAFQQRVWQALRR
                     IPAGSTASYTQLARQLGSPNSVRAVAQACAANPLAVAIPCHRVVRIDGGLAGYRWGVE
                     RKRALLAREANA"
BASE COUNT         1290 a         2730 c         2771 g         1133 t
ORIGIN      
        1 ccagggtccg gcgagaaaca gcgccaggat gccgagctgc acgctgcgcc gcgccagcaa
       61 ccagcgattg cgcgccagca gcccgcgcct tgcggcggcg attgcggacg gcgcgggggc
      121 attcatcgcg ccgccatttt atccagcgga ggcgagatcg tgctgggcca gcgccacggg
      181 cagcaccttg atcgaggcct cggcggtgac gcaatcgtgc tcgcacttgc cgcacccggt
      241 gcacttgtcc gcgtgcacgg tgggctcgaa atagctgcgc ccgttggagt gacgccgctc
      301 catggtgatg gcctcgtcct tgatcgggca ggcgaggtag caggcccggc agttggcgcc
      361 gcgcgcgacc gcatagcagg tgtcgcggcc ggtcagcacc gccacgccca tcttcgcgtc
      421 gcggatgtcg gtcagcgcct tgctcagcgc atcggtgggg caggccgccg cgcacgggat
      481 gtcctcgcac atctcacagg cgatctcgcg cgcgacgaag tacggcgtgc cggtagcgac
      541 gtcctggccc agttcggcca ggtgcaacat gtcgaacgga caggcgcgca cgcacagtcc
      601 gcagcgcacg cacgccgcgc cgaagcggtc ctccggcagc gcgccggggg gccgcagcgc
      661 ccacgccggc agcgccttgg actgccggct gtagcttgtc agcagcaacc cgactacgcc
      721 ggccgagccg gccagcccgg cgcagtcctg catgaacttg cgccgtccca agcgtcgtgc
      781 gtcgtctgcc atcggagcct ccccgcccgt ttcagctagc ggccgcggcc cgctcgatgc
      841 gcagccgcgg atcgactgca tagtacagaa gatcgaccgc caggttgatc accacgaaga
      901 acagcgcgat cagacacagg tacgcggcca tcaccggcac gtcggcgaac atcaccgact
      961 ggatgaacag cagtcccatg ccgggccact ggaacacggt ctcggtgatg atggcgaagg
     1021 cgatgatcga gccgagctgc agcccggtga cggtgatcac cggcaccagc gtgttcttga
     1081 gcgcgtgccc gaagtgcacg gcgcgatcgc tgaggccgcg cgcgcgggcg aaacggatgt
     1141 agtcggtgcg cagcacctcc agcatctcgg agcgcaccag gcgcatgatc agcgtcatct
     1201 ggaacaggcc gagcgtgatc gacggcagga tcagcgcctt gagcccggat gcggtgagca
     1261 gcccggtggt ccaccagccg atgctcacgg tctcgccgcg cccgaacgaa ggcagccagc
     1321 ccagctccac cgagaacacc aggatcagca ggatgccgat caggaacgtg ggcagcgaca
     1381 cgccgaccag cgacactgcc aggaacacgt gcgacagcca cgcggttcgg cgcaatgccg
     1441 tgtatacgcc catcggcacg ccgaccacga gcgccagcag ggcggccgcg agcgacagct
     1501 cgagcgtggc cggcaggcgc tcggcgatca gcgtcgacac cggccgcagc tggcgcagcg
     1561 agaagccgaa gtcgccatga agcgcgcgcg ccacgaagcc ggcgtactgc gcatagaagg
     1621 gccgatccag ccccagctcg cgcgtcagcc gggcgcggtc ctcgggcgtg gcttcctgcc
     1681 ctagcatgaa caccaccggg tcgccgacgt accggaacag ggcgaaggcg atgatgccca
     1741 ccgtgagcat caccgcgacc gcctgggcca ggcgccggag aacgaaggca aacatctgga
     1801 ggtgtggtcg cagaccgccg cgcaacgctc ggctggagcg gcgcgggtct gcccggatgt
     1861 tgccgaagtc ggtcgcgggc gtcaacgcgc cgcgcgggaa cgctcgctgg tgatccgcgc
     1921 aaagaaaacg gccgaccccg atggggtcgg ccgaaagccc cgcggaaagg aggaaacgcg
     1981 ggggaggagg acttcagttc acgcgcccgc tcgcgccgtg ctgtgccagc gccgtgccgc
     2041 tgcaggcggc ggccagaagc gccgcctggg gatcgcccgc gtgcacctgc gcgaatgcgg
     2101 gcgcagtcgc gcgatcggcc cacaccgcat cgccgtggcc gagcgcgccc tggtgcagga
     2161 tccactgtgc gatcgcgtaa gtttcttcgc tgcagttgaa cacgtagcgc agcgaacggg
     2221 agcgatgcgg ataccagccc tcgcccacgt ggcgcacttc ggggtagctc tccagcaccc
     2281 acagcgaggc ggcgaccggg ttgcgcgcga cgcggccacc gtctgtgaag gtgagctgct
     2341 gatcgtcgag cgataccagc ttccagtcct gggcggctgc actgagagac atcgcggccg
     2401 tcgccacggc ggccgcaaca acaagtttga gtttcatgtc gatctcctga gaagggttga
     2461 atcggggggt tcagttcaag gcgaggccgc cgtcgatgcg caccttggcg ccgagcagga
     2521 agctggaatc ggcggaggcg aggaacaagg cggcgcgcgc cacttcctcg gcggtgccca
     2581 gccgcttcag cggcaccagg gcgctcatgc cgtcgacctg ctccgcggtc aggccgatct
     2641 tgccgaagat cggcgtctcg atcggtcccg ggctgacctc gttgacgcga ataccgcgcg
     2701 gcgccagctc gttggccagc gtgcgcgcca gcgacgacag tgcggccttg gttgccgagt
     2761 aaacgctggt gctggccatc ccgaggtcgg cggcgaccga ggtggtgagc acgatcgaac
     2821 cgccgctcga catcagcggc aggatctgct ggatggtgaa gtacgcgccg cgcacgttga
     2881 cgtcgaactg ctcgtcgaag gcctgcggcg tcacgttctc gatcggcagg aagcgcgcga
     2941 tgccggcgtt gacgaacagc acgtcgatgt ggccggccct gttcttcaag gtgctcgcga
     3001 gcgcctggac gtcggccagg tcggcggcgt cggagggcag cacctcgacc tccggcggca
     3061 gttgcttgcg cgcttccgcg atgccggcct cgctgcggcc agtcacgaac accctggcac
     3121 cttcggcgtg gaacaacttc gccgcggcca atccgatccc ggtggtgccg ccggtgatca
     3181 atgccacttt gtcttgcagt ttcatctttg cgcctcctat attggaacga tcggtctgat
     3241 caaggtaaaa aaattcaccg cgtcacggcc gcgagcgcga cctccacgat gtcgttcaac
     3301 actttcggat ctcgggtcgc cttggcggtc accgccagcc cgttcatcgt gttcatcagg
     3361 aaccgggcga gctgcaacgg cgggctgtcg gccggaatct cgcccgcctg ctgcgcccgc
     3421 accagcagcc cggacaacgc gccttcgatc tggcgcgcgt tggccgcgca agcttgcgtg
     3481 accgccgggt cgtggccggc cagttcggtg atggtgttgg cgatgaagca gcccttgaaa
     3541 tcgccgctca gcagattgtc cgcgaacgcg tgcaggaact cgcgcagcac ctcgagggcc
     3601 gggccctcgc gctcgagcag acggcgcacg ggcgcgccct ggatctcgcg gtagcgctcc
     3661 agcgcggaca ggaacagctg gtcctttccg ccgaaggtgt cgtacaggct ctgccggttc
     3721 acgccgagcc gatcgaccag gtcctggatg ctggtggcgt gataaccccg ctcccagaaa
     3781 agctgcatcg cctcgtccag ggccgcgtgc tagtcgaact gcttggtgcg taccatggcc
     3841 gccatcaaac catatctgga acgatcggtc aagacccggc cgcacgcagc ggacgcgaga
     3901 gcatcatcgc cggtaccagc agcaccgcct gcaccaggaa gaacaccacg gcaaaggaca
     3961 ggaaatccgg gcgccccagc acgttcaccg cgatcagcgt ggcgatgctc aggctgcgcg
     4021 aggggtaggc cgcggccatc gtgaccgtgt cgatccggct ccaggcgaaa gccctcgcga
     4081 tggcgagccc cagggccagc gccccggcgg tgaacagcag gccggcgacg actatcggca
     4141 gcagattgcg cgcgatcgtg ccgccctggt ccaccagcac gatggcgatc agcgcggcga
     4201 gcgagagcag gctcaggcgc tggaagcgca cgcgctggcg ttccacgaac cccggcgcaa
     4261 agtgccgcac cagcatcccg gcggcgatcg gcagcagcag cccgatcacc acctgctcca
     4321 tcaccttgcc ggcgggcagc gagaagcctg cctggcgctc gagcagcaag ccgaaggcga
     4381 tctgaacggc gatcggcgtg gacaccagcg ccagcacgct ggaaacggcc gtcagggtca
     4441 gcgccagtgc gatgttggcg cgcgccagga gacagtaata gttggagatg atggcctgcg
     4501 gcgcggcggc gatcagcacc agcccccccg tgaccgcggg ctccgggcgc aggaccagga
     4561 tcagcgccac gccgagcagc ggcagcaaca gcacctgccc gagcagcgcc accgccacgt
     4621 gtacaggata gtgcagcacg cgcttgaggt ccgccggcgt gagctccagc ccgacgacgg
     4681 tcatcgcgta cacgatgatc agcggcaggc cgacttcgac gagcgggctc atggtgtcgc
     4741 atgttcgcag cgcgagccga ccgtggcaag cagacccgcg cgcggcggcg agagccgcgt
     4801 ttgcacgata atccgaccgc ttgctacggg agaaaacaag atgcgggcga tctggggcat
     4861 gctgctggcg ttgctggcgg cgctggtggt ggtgctggcg gtcaatacgg cacggtccgg
     4921 ctcgcggcag atcgaggtgc cgcgcgcgga tgagcgcttg ccggaactgg agctggccgt
     4981 gcagcgcctg gcgcaggcga tccgactgca gaccgtctcg cagcaggacc cggaccgctt
     5041 gccgatcggt catttcgagg cgttgcatcg cctgctgcgg gacggtttcc cgcgcctggg
     5101 caaggcgctg aagcgcgagc gcatcggcgg gtcgagcttg ctctacgaat ggccgggcgc
     5161 ggatcccgcc gcgaagccga tcttgctgct cgcgcacctg gacgtcgtgc ccgccgagga
     5221 gggcagcttg cagcagtggg cgcatgcgcc cttctccggc gccgtggcgg acggcttcat
     5281 ctgggggcgt ggcacgcgcg acgacaaagg cgccgtgatg gcattgcacg aagcggtgga
     5341 agcgctgctc gcgcagggct tccagccgcg gcggacggtg tatctcgcct tcggccacga
     5401 cgaggaggtc ggcggcacgc gcggcgcagc gcagatcgcg caggcgctcg ccgcgcgcgg
     5461 cgtgacgctc gagtcggtgc tcgacgaggg catgcccgtc acgcacggtc tcctgcccgg
     5521 catccagcag ccggtggcgt tgatcggcat cgccgagaag ggctacgcga cgctcgagct
     5581 ggtggcgcaa ggcgagggcg ggcattcctc catgccgccg ccgcaaaccg cggtgacgat
     5641 cctgggcgcg gcgctggcca agctcgaagc gtcgcgcttt gcggccacgc tcaccgagcc
     5701 gctgcgcgag cagttggcat ttctcgggcc cgagcagggg ctgctgcgcc gggtcctgtt
     5761 cgccaacctg tggctgttcg gcggcgtggt gaaagggcag ctcgccaact cgcgcgccgg
     5821 caacgcgctc atccgcacca cggtggcgcc caccatgctg gagggcagcg tcaaggagaa
     5881 cgtgctgccc gcgcgcgcac gcgccctggt gaattttcgc ctgctgccgg gcaccagtat
     5941 cgacgcgttg acgcgtcggg tggtggagat cgtggacgac ccgcgcgtcg cgatcgcgcc
     6001 cgcgggcaca tcgcgcagcg agccctcgcc ggtgtcgagc acccgatccg aggccttccg
     6061 cagtctgcag cgcgcggtgg aagcggtgtt ccccgaagcg ctggtagcgc cctccctgct
     6121 ggtcgccgcc accgactcgc gtcacttcgc gccgctcgcc gaatcggtgt ttcgcttcct
     6181 gccggcgcgc accggccccg acgacctgcc ccgctatcac ggcgtgaacg aacgcctggc
     6241 cgtggacaac tacgccgagt tcatcggctt ctacatgcgc tacctgcgcg aggcggccgg
     6301 cgggtgatgg tcaactggcg tcgtggaaga tgatgccgag cgtgtggcga tgtcccgagc
     6361 gcacgcggct gacgccatgg cgcaggttga cccggtatgc accgcgcgtt ccctgcaccg
     6421 ggcgctggtg aacggcgaac accaccgcat cgccctgccg cagcggcacc acttcggggc
     6481 gcgactgcat gcgcgggcgc tgctcggtga tcacgaactc gccgccgctg aagtcgcgcc
     6541 cgggctccga cagcaggatc gccacctgca gcgggaagaa gtgctcgccg tacagatcct
     6601 gatgcaggca gttgtagtcg tccgcgccgt actgcagcag cagcggcgtc ggccgccgct
     6661 ggccggccgc gtggcagcgc ctgatgaact ccgcgtgctc gtccgggaag cgaacgcggc
     6721 tgcgcatcgc cgcctgccag cggttggcga tcggcgccag ccgcgcgtag agggggctgc
     6781 gcaggccggc gacgatcccc ggcagggggt agcgaaaata tttgtactcc ccgcgcccgt
     6841 aaccgtgccg cgccatcacc acgcggctgc ggaagacgcc gtcgtccgga tacaactcgg
     6901 ccaaggcggc gcactccgcc ggcgtgatca gccgctcgat cgtcgcgttg cctcgggcgt
     6961 cgagatcgcc ggatacgcgg tcccagtcga tcgcctccac cctgccggcg acgtcgctcg
     7021 cgcaagccag ggtcgcgacg ctgccgtcgc gcgcgttcac gcgtttgcct cgcgcgccag
     7081 cagcgcgcgc ttgcgttcga cgccccagcg gtaaccggcc agcccaccat cgatccgcac
     7141 cacgcgatgg caggggatcg ccaccgccag ggggttggct gcgcaggcct gcgccacggc
     7201 gcgcaccgag ttgggcgagc cgagctgccg cgcgagctgc gtgtagctcg ctgtcgagcc
     7261 cgcggggatc cttctcagcg cctgccacac gcgctgctgg aacgccgttc cgcgcacatc
     7321 gagcggcagg tccagaccca gcccgggggc ttcgacgaaa cccaccaccc tggccacgat
     7381 gcgctcgaag tcccggtcgc cgccgatcag gtcggcgcgc gggaacctgt cctgcagatc
     7441 gcgcgccagg gcttcgggat catcgccgag gagaatggcg cacacgccgc gctcgctggc
     7501 cgcgaccaga atggatccca acgagcactc gccgatggcg aagcggatct gcgtgtccgc
     7561 cccgccggcg cggtagcggg atggcgtcat gcccagcacc tggtccgagt tttcgtagaa
     7621 gcggccgttg gagttgtagc cggcgtcgaa gatcgcagtg gtgaccgtgc ggctgcggcg
     7681 aagctcgttg cggacgcgct tggcgcgatg cgccgcggcg taggctctgg gcgtgaggcc
     7741 ggtggcggcc ctgaacacgc gatggaagtg gtacgcgctc agccctgcgc gccgcgccag
     7801 ctcctccagg ctcggaaccg tctcggcgga ttcgatcagg cggcagatct cggccactct
     7861 tcccgcatgc tgctcggcca gcgcaggctg gtccggtctg cagcgcttgc agggccggaa
     7921 gccg
//
LOCUS       DASPGE010000407         3512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_812637, whole genome shotgun sequence.
ACCESSION   DASPGE010000407 DASPGE010000000
VERSION     DASPGE010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812637"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..967
                     /locus_tag="VD791_13445"
     CDS             95..967
                     /locus_tag="VD791_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXV09032.1"
                     /translation="METSSPNIASATRAPKVLSRAQVEAFARNGYHFPIRALSAEQAG
                     EYRARLESVEAKLGTPVMKTGLRNKPHLALAWADELIRHPSILDTVEDILGPNLLVWS
                     SSFFIKEARDPHYISWHQDSTYWGLSHPDVVTAWLALSVSDRSNACMRVVQGTHLMDQ
                     LPHQDTFAADNLLTRGQEVMVQVDESKVVDIELQPGEFSLHHVRIVHGSDPNPAAYRR
                     IGLAVRYVPTYVKQTAGPRDSAMLVRGVDNYHHFELEPRPKADLDPEALAAHQRVTEE
                     ANKILYRGTDKAPK"
     gene            964..2022
                     /locus_tag="VD791_13450"
     CDS             964..2022
                     /locus_tag="VD791_13450"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXV09033.1"
                     /translation="MKRPLPRALLLIAVLLLAGCGQQKAGDAGQQSASGQPGMHWKMQ
                     SYWQSGTLPQTLFEEFAKRVTDLSGGRLAVEALATGAVVAPTESLDAVGSGVLDGHNG
                     GPAYFTGKDAAFALLGDPQGAFETPYQFQMWMEYGGGNELARELYATYGVHFVGGVWY
                     GQECLVSKRPLRSLADFKGLKIRAPQGMNGEIFTQLGAAPVQLPGSEVYTALERGVVD
                     AADWGTLSMNDDLGYHKLAKYPTYPGFHSMPMGEISVNKAKWDALPADLKAIIESATY
                     EFARAMIQRNYLADAKVAVERGQRGFEPTDMPLEERQKFREIARSVWKEYAARSPLAQ
                     KVHASQIAFLQQLGLLRE"
     gene            2037..2531
                     /locus_tag="VD791_13455"
     CDS             2037..2531
                     /locus_tag="VD791_13455"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXV09034.1"
                     /translation="MPDRITRLVGAVGEAASWLFVAIVAITAYEVAMRYVFNAPTIWV
                     HELAIALAATCFVIGGPVVHQRQQHISISVIYERLGPAAQRWARALSSLLTLVFCLFL
                     SYAATQQAWVALKARETTGTALNWPIPAFLKTLFALAAVVLVLQTLGHLLQDIRRLRA
                     RGLH"
     gene            2549..>3512
                     /locus_tag="VD791_13460"
     CDS             2549..>3512
                     /locus_tag="VD791_13460"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXV09035.1"
                     /translation="MSIEWITVLMFGSMLVLLALGLPLAFVTGLIGVTFCIGLFGVNG
                     LQLVASRIYSFMNEYALVSVPMFIMMASLMERSGVAKDLFDAMRVWAGKLPGGLAIQT
                     MVAATIMGAMTGIIGGEIVLLGLIALPQMLRLGYERRLAIGTICAGGSLGTMIPPSIV
                     LIIYGLTVNVPIGELFTANIVPGLLLAGIYMLYIFVRCQIDPRLGPPAPESEWRIPLG
                     RKIAQLKGLALPMLVIATVMGTIYAGVASVTEAAGMGVVGTMIASAVRGELKWVHMRE
                     SMRQTMRTCGMLLWLSFGATAMIGVYNLAGGPAFVKQVMTGMDIA"
BASE COUNT          565 a         1180 c         1209 g          558 t
ORIGIN      
        1 ggaagaaggc cttgtctccc cggattccgc ttacgctcca tccgggctac gattactacg
       61 attggcacga ttacagagca gtcgaggaca gcgcatggaa accagcagcc ccaacatcgc
      121 ctccgcaact cgcgccccca aggtcctgag ccgggcgcag gtggaggcct tcgcccgcaa
      181 cggctaccac ttcccgatcc gcgcgctgag cgccgagcag gcgggcgagt atcgcgcgcg
      241 gctcgagagc gtcgaggcga agctgggcac accggtgatg aagaccgggc tgcgcaacaa
      301 gccgcacctg gcgctcgcct gggccgacga gctgatccgg cacccgagca tcctggacac
      361 ggtggaggac atcctggggc cgaatctcct cgtctggagc agcagcttct tcatcaagga
      421 agcgcgcgac ccgcattaca tctcctggca ccaggactcg acctactggg gcctgagcca
      481 ccccgacgtg gtaaccgcct ggctggcgct gtcggtcagc gatcggtcca acgcctgcat
      541 gcgggtcgtg cagggcacgc acctgatgga ccagctgccg caccaggaca ctttcgccgc
      601 cgacaatctg ctcacgcgcg ggcaggaggt gatggtgcag gtggacgaga gcaaggtggt
      661 cgacatcgag ctgcagccgg gcgagttctc gctgcaccac gtgcgcatcg tgcacggctc
      721 ggacccgaat ccggccgcct accgccgcat cggcctggcc gtccgctacg tgcctaccta
      781 cgtcaagcag accgccgggc cgcgcgacag cgcgatgctg gtgcgcggcg tggacaacta
      841 tcatcacttc gagctggagc cgcggcccaa ggccgatctc gaccccgagg cgctggcggc
      901 gcaccagcgc gtcaccgagg aagccaacaa gatcctgtac cgcggcaccg acaaggcacc
      961 gaagtgaagc gccctctccc cagagcactg ctgctcatcg cggtgctatt gctcgccggc
     1021 tgcggccagc agaaggccgg ggacgcgggg cagcagtccg cgtccggcca gcccggcatg
     1081 cattggaaaa tgcagtcgta ctggcagagc ggcacgctgc cgcagacgct gttcgaggaa
     1141 ttcgccaagc gcgtcaccga tctgtccggc gggcggctgg cggtcgaggc gctcgccacc
     1201 ggcgcggtgg tggcgccgac ggagagcctg gacgcggtgg gctcgggcgt gctcgacggg
     1261 cacaacggcg gcccggccta cttcaccggc aaggacgccg cgttcgcgct gctgggcgac
     1321 ccgcagggcg ccttcgagac gccgtatcag ttccagatgt ggatggagta cggcggcggc
     1381 aacgagctcg cgcgcgagct gtacgcgacc tacggcgtgc atttcgtcgg cggcgtgtgg
     1441 tacggccagg aatgcctggt gtcgaaacgg ccgctgcgct cgctcgccga cttcaagggc
     1501 ctcaagatcc gcgcgccgca aggcatgaac ggcgagatct tcacgcagct gggcgccgcg
     1561 ccggtgcaac tgccgggcag cgaggtgtac acggcgctgg agcgcggcgt ggtggacgcg
     1621 gccgactggg gcaccctgtc gatgaacgac gacctgggct accacaagct cgccaagtat
     1681 ccgacctatc ccggcttcca ctcgatgccg atgggcgaga tctcggtgaa caaggcgaag
     1741 tgggacgcgc tgcccgccga cctcaaggcg atcatcgaga gcgccacgta cgaattcgcg
     1801 cgcgccatga tccagcgcaa ctacctggcc gacgcgaaag tggcggtgga gcgcggccag
     1861 cgcggcttcg agccgaccga catgccgctc gaggagcgcc agaagttccg cgagatcgcg
     1921 cgcagcgtgt ggaaggagta tgctgcgcgc agccccttgg cgcagaaggt gcacgcctcg
     1981 cagatcgcct tcctccagca gctggggctg ctcagggaat agaccccgat acgccgatgc
     2041 ccgaccggat cacccggctg gtgggcgccg tgggtgaggc ggcgagttgg ctgttcgtcg
     2101 cgatcgtcgc catcaccgcc tacgaggtgg cgatgcgcta cgtgttcaac gcccccacca
     2161 tctgggtgca cgagctggcg atcgcgctgg ccgccacctg cttcgtgatc ggcggcccgg
     2221 tggtgcacca gcgccagcag cacatcagca tcagcgtgat ctacgagcgc ttgggcccgg
     2281 cggcgcagcg ctgggcacgg gcgttgagct cgctgctgac gctggtgttc tgcctgttcc
     2341 tgagctacgc cgccacgcag caggcctggg tcgcgctcaa ggcgcgtgaa accaccggca
     2401 ctgcgctcaa ctggccgatc ccggcgttcc tgaagacgct attcgcgctg gccgccgtgg
     2461 tgctggtgct gcagacgctg ggccatctgc tgcaggacat ccgccggctg cgcgcgcgcg
     2521 gcttgcactg agcgggatcc gcacgatcgt gagcatcgag tggatcaccg tgctgatgtt
     2581 cggctcgatg ctggtgctgc tggcgctggg gctgccgctc gccttcgtca ccggcctgat
     2641 cggcgtcacc ttctgcatcg ggctgttcgg cgtgaacggg ctgcagctcg tcgccagccg
     2701 catctacagc ttcatgaacg agtatgcgct ggtgtcggtg ccgatgttca tcatgatggc
     2761 ctcgctgatg gagcgctccg gcgtggccaa ggacctgttc gatgccatgc gcgtgtgggc
     2821 cggcaagctg ccgggcgggc tcgccatcca gaccatggtg gccgccacca tcatgggcgc
     2881 gatgaccggc atcatcggcg gcgagatcgt gctgctgggg ctgatcgccc tgccgcagat
     2941 gctgcgcctg ggctacgagc gcaggctcgc catcggcacg atctgcgccg gcggctcgct
     3001 cggcaccatg atcccgccgt cgatcgtgct gatcatctac ggcctgacgg tgaacgtgcc
     3061 gatcggcgaa ctgttcaccg ccaacatcgt gccgggcctg ctgctggccg gaatctacat
     3121 gctgtacatc ttcgtgcggt gccagatcga tccccggctc ggcccgccgg cgccggagtc
     3181 cgagtggcgc atcccgctcg gccgcaagat cgcgcaactc aaggggctgg ccttgccgat
     3241 gctggtcatc gccacggtga tgggcacgat ctacgcgggc gtggcttcgg tcaccgaggc
     3301 cgccgggatg ggcgtggtcg gcaccatgat cgcttccgcc gtgcgcggcg agctgaagtg
     3361 ggtgcacatg cgcgagtcga tgcggcagac catgcgcacc tgcggcatgc tgctgtggct
     3421 gtccttcggc gccaccgcca tgatcggggt gtacaacctc gccggcgggc cggcgttcgt
     3481 caagcaggtg atgaccggca tggacatcgc gc
//
LOCUS       DASPGE010000408         4236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1280694, whole genome shotgun sequence.
ACCESSION   DASPGE010000408 DASPGE010000000
VERSION     DASPGE010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280694"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VD791_13465"
     CDS             <1..397
                     /locus_tag="VD791_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV09036.1"
                     /translation="SFQLLPAMTALENVMLPLELAGATDAQGPARAMLARVGLGDRLH
                     HYPKQLSGGEQQRVAIARAFVTRPKLLFADEPTGNLDTTTGGQVIDLLFELNRESGTT
                     LLLVTHDEAITGRCDRVLRLHAGRLVENA"
     gene            399..2897
                     /locus_tag="VD791_13470"
     CDS             399..2897
                     /locus_tag="VD791_13470"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXV09037.1"
                     /translation="MSLLTEIRLAWKFLKRDYRAGEITLIAVAIVIAVAAVTTVGFFT
                     DRVQRVLEGEANRLLGADLVISDTRPLDPALRQEASRRGLAVAEIIRFPSMVVVGERT
                     VLTDVRAVSPGYPLRGELRIAERLFGRDRAAGTIPGPGSVWVDARLHTALDLQAGARI
                     GLGNSRLAVSGVVTHEPGVELGFLSGQPRLLLNAEDLVATGLVQPASRVRYRLQLAGE
                     ASAVDAYRNWAQARLRPGTRMEGVRDARPEVRSALERAEKYLNLAALTSVLLAAVAIA
                     LSARRYLQRHLDGCAMMRCLGASQALIVRLYVTHFTVLGALAAAAGIAAGAGAQAVLV
                     AWLGQVVAVSLPGASLMPAVHGAAVGWLLLLGFALPPLVSLAQVPTLRVLRRELGMPR
                     RRGLAAYVLGAGVVAVMILWRAQELRLGLTVLGWFVAATACACALTWALLHSVGALRG
                     HGVSWRFGVTNLRRRTLGTILQVVALAMGIMALLTLTVIHNDLLRTWRESLPRNAPNR
                     FIINIQPDQVAAIARFFSERRLAAPELFPMVRGRLIDIGGRSVKADDYEDDRARRLIT
                     REFNLSWATRLQADNRVVVGRWWEGAPGQAGQFSVEKGLADALGIRMGDPLTFDIAGT
                     PVTATVTSLRTVDWDSFNVNFFVVAPPGLLDKYPATYVTSFHLEGPRAELLTALVKAF
                     PNIVLIDVAQVLSQVRRMLDQAALGVQFVFLFTLAAGLVVLYAAVASTQDERLYQATI
                     MRTLGASRAQISRAHLAEFGLTGAAAGFVAAAGASGLAYFIAHRFLHLAYAPDPSVWL
                     IGIGGGALGVAAAGYLGTRRVLAAPPLKVLRAIG"
     gene            2913..4091
                     /locus_tag="VD791_13475"
     CDS             2913..4091
                     /locus_tag="VD791_13475"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HXV09038.1"
                     /translation="MPDILWLVLAFAVLAVLVVLIARLAGQATAVRRLSEELAPVLEE
                     RHRAMLTDLHGGLAQQSDRLNSRLSEELTQTRDTLHRLQLSLAASLGETTDKLNARID
                     QRLEQIAGKVDERLEEGFKKTNETFVNVMQRLATIDEAQKKIDTLTGSVVGLQELLGD
                     KRSRGAFGEVQLEAQVRNTLPPSAFEMQVTLSNGMRVDCVLKLPEPVGLLCVDSKFPL
                     ENYHRMVAPGGNDVDRALAQRQFRADVRKHVDDIARKYILPNETAEGAVMFVPAEAVF
                     AEIHAYHAEVVEYANSKRVWIVSPTTLMAVLNTARAVLKDVETRKQVHLIKDALVRLS
                     VEFNRFDERLRRLADHIRQAHDDAQKIQITGDKISQQFQRIEAAELDAPEAAKLKVVE
                     "
BASE COUNT          627 a         1376 c         1502 g          731 t
ORIGIN      
        1 gtcgttccag ctgctgccgg caatgaccgc gctcgagaac gtgatgttgc cgcttgagct
       61 tgccggtgcg accgatgcgc aggggccggc gcgggcgatg ctcgcgcggg ttggcctcgg
      121 cgatcgcctg catcactacc ccaagcagct ctcgggtggt gagcagcaac gcgtggcaat
      181 cgcgcgtgcg tttgtgaccc gccccaagct gctgttcgcg gacgaaccga cggggaatct
      241 cgacacgacg acgggcgggc aggtgatcga tcttctgttc gaactgaacc gcgaaagcgg
      301 cacgacattg ctgctcgtga cgcacgacga ggcgatcacc gggcgctgcg accgcgtgct
      361 gcgcctgcat gccggccgcc tggtggagaa cgcctgacat gagcttgctc acggaaatcc
      421 gcctggcgtg gaaattcctg aagcgcgact accgcgccgg ggagatcacg ttgatcgcgg
      481 tggcgatcgt catcgccgtg gcggctgtga ctaccgtcgg gttcttcacc gaccgggtgc
      541 agcgggtgct cgagggggag gcgaaccgtt tgctgggagc tgacctcgtc atctccgaca
      601 cccggcccct tgatcccgcg ctgcgccagg aagcgtcacg ccgcgggctg gcggtcgccg
      661 aaatcatccg gtttcccagc atggtcgtgg tcggcgagcg cacggtgctg actgacgtcc
      721 gggctgtatc ccccggctac cctctgcgcg gagaattgcg cattgccgag cgcctgtttg
      781 gtcgcgaccg ggctgccgga accatccccg gccccggaag tgtgtgggtg gacgcgaggc
      841 tgcataccgc gctcgatctg caagcggggg cgcgcattgg gctcggcaac agccggctcg
      901 ctgtcagcgg tgtggtgacc cacgagccgg gcgtggagct cggtttcctg agcggccagc
      961 cgcgtctcct gctcaatgcg gaggatctcg tggcgacggg cctcgtgcaa cccgcgagcc
     1021 gcgtccgcta ccggctgcag cttgccgggg aggcgagcgc cgtggacgcg taccggaact
     1081 gggcgcaggc ccgcctgcgc ccgggcacgc gcatggaagg cgtgcgcgac gcgcgacccg
     1141 aggtgcgctc tgcgctcgag cgcgccgaga agtatctcaa cctggcggcg ctgacgagcg
     1201 tgctgctcgc cgcggtggca atcgcgctgt cggcgcggcg ctacctgcag cgccatctcg
     1261 acggctgcgc catgatgcgc tgcctgggcg cgagccaggc gctcatcgtc cggctctacg
     1321 tcacccattt cacggtgctg ggtgcgctcg ccgccgcggc cggcatcgcg gccggggcag
     1381 gcgcccaggc agtgctcgtc gcgtggctcg gccaggtcgt cgcggtgagc ctgcccgggg
     1441 cgagcctcat gccggcggtg cacggggccg ccgtcgggtg gttgctgctg ctgggattcg
     1501 cgctgccgcc gctggtgagc ctcgcccagg tgccgacgct gcgcgtgctc aggcgggagt
     1561 tgggaatgcc caggcgccgg gggttggccg cgtacgtgct cggcgccgga gttgtcgccg
     1621 tgatgatact gtggcgcgcc caggagctgc ggctcggcct gacggtcctc ggctggtttg
     1681 tcgcggccac ggcgtgcgcc tgcgcgctca cctgggcgct gctccattca gttggcgcct
     1741 tgcgcgggca cggcgtgtcc tggcggttcg gcgtgacgaa cctgcggcgc cgcacgctcg
     1801 gcaccatcct gcaggtcgtg gcgcttgcga tgggaatcat ggcgctgctc acgctcacgg
     1861 tcatccacaa cgatctgctg cgcacctggc gcgagagcct gccgcggaac gctccgaacc
     1921 gcttcatcat caacattcag ccggaccagg tcgcggccat cgcgcgcttc ttctcggagc
     1981 gacggctggc ggcgcccgag ctctttccta tggtgcgcgg gcggctcatc gacatcggcg
     2041 ggcgcagcgt gaaggcggat gactacgagg acgatcgggc gcggcgcctg attactcggg
     2101 aattcaatct gtcatgggcg acccgcctgc aggcggacaa tcgcgtcgtc gtggggcgct
     2161 ggtgggaggg tgcgccgggg caggccgggc aattctcggt ggagaagggt ctcgccgatg
     2221 cgcttggcat ccgcatgggc gatcccctga catttgatat tgcggggact cctgtcaccg
     2281 cgaccgtaac aagcctgcgc accgtcgact gggacagctt taacgtgaac ttctttgtcg
     2341 tggcccctcc cggcctgctg gacaaatatc ccgcgaccta cgtgacgagc ttccacctgg
     2401 aggggccgag ggccgagttg ctgaccgcgc tggtgaaggc atttcccaac attgtcttga
     2461 tcgatgtcgc gcaggtgctc tcccaggtgc ggcgcatgct ggatcaggcc gcgctggggg
     2521 tgcaattcgt gtttctgttt acgctcgccg ccgggctggt ggtgctgtat gcggccgtcg
     2581 ccagcaccca ggacgagcgg ctctaccagg caacgatcat gcgcacgctg ggggcgagcc
     2641 gcgcgcagat ttcccgcgct caccttgcgg agttcgggct gaccggtgcg gcggcgggat
     2701 tcgtggcagc ggcaggggcg agcgggctgg cgtacttcat tgcgcaccgt ttcctgcacc
     2761 ttgcctatgc gcccgacccg tcggtgtggc tgatcggaat cggcggcggc gcgctcggcg
     2821 tcgcggcggc gggctacctc ggcacgcggc gcgttctggc ggcaccaccg ttgaaggtcc
     2881 tgcgcgccat tggctgatat aatttgcagg cgatgcccga catcctgtgg ctggttctgg
     2941 cgttcgctgt cctcgctgtg ctggttgtgc tgatcgcgag gctggcgggc caggcgaccg
     3001 cggtgagacg gctgtccgaa gagctagctc cggtgctcga ggagcggcac cgggcgatgc
     3061 tgaccgacct tcacggcggc cttgcccagc agtccgaccg cctgaattcc cgtctgtccg
     3121 aggagcttac ccagacccgg gataccctgc accgcctcca gctctcgctt gccgcgagtc
     3181 tcggtgaaac caccgacaag ctgaacgcca ggatcgacca gcgcctcgag cagatcgccg
     3241 ggaaggtgga cgagcgcctc gaggagggct tcaagaagac caacgagacc ttcgtcaacg
     3301 tgatgcagcg cctcgccacc atcgacgagg cgcagaagaa gatcgacacg ttgaccggca
     3361 gcgtggtggg cctgcaggag ctgctcggtg acaagcgttc gcgcggtgcc ttcggcgaag
     3421 tgcagctcga ggcgcaagtc cgcaacacgc tgccgccgtc ggcgttcgag atgcaggtca
     3481 cgctttccaa cgggatgcgg gtggactgcg tcctgaagct tccggagccg gtgggtctgc
     3541 tgtgcgtgga ttcgaaattc ccccttgaga attaccaccg gatggtcgcg ccggggggaa
     3601 acgacgtcga ccgcgcgctc gcccagcgcc agtttcgcgc cgacgtgaga aagcatgtcg
     3661 acgacatcgc gcggaagtac atcctgccga acgagaccgc ggagggcgca gtgatgttcg
     3721 tgccggccga ggcggtgttc gcggagatcc acgcctatca tgccgaggtc gtggaatatg
     3781 ccaactcgaa gcgcgtctgg atcgtgtccc cgacgacgct gatggcggtc ctcaacacgg
     3841 cgcgcgccgt gctgaaggat gtcgagaccc gcaagcaggt acacctgatc aaggatgcgc
     3901 tcgtgcgcct ctcggtcgag ttcaaccggt tcgacgagcg cctgcggcgg ctcgcagacc
     3961 atatccggca ggcccacgac gacgcgcaga agatccagat caccggcgac aagatttcgc
     4021 agcagttcca gcgcatcgag gccgccgaac tcgacgcgcc ggaggcggcg aagctcaagg
     4081 tcgtcgaata ggccgggcgc ttccgtgcag tggctcagca actggcggcg cgagcgcata
     4141 ctccggcgcc atgccctcga tgaacgcttg tggcgctcgg tggtggaccg ctatcctttc
     4201 acccgcgcgc tgtcgggagc agagcagtcc cggctg
//
LOCUS       DASPGE010000409         3447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_110773, whole genome shotgun sequence.
ACCESSION   DASPGE010000409 DASPGE010000000
VERSION     DASPGE010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110773"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1019)
                     /locus_tag="VD791_13480"
     CDS             complement(132..1019)
                     /locus_tag="VD791_13480"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HXV09039.1"
                     /translation="MATWNLQWLIAPETFERLAASCRDEPPSGERRWIPCDIAAGERA
                     MRRTAADFAKLREYARRLDADVVALQEVDGPQAARLVFPGHDFCFTRRVHVQNVGFAI
                     RPGVPFRCADYPDLGLEGSRVRWGADLTLFPGSQREVRVLAVHLKSGCHGERLSSERG
                     DCRTLAEQVPVLERWIDARARDGAPFAVAGDFNRRFSSERRQARDRLGRLTAMWPELD
                     DGSPPEADLTDATPVRRDARCFAQASRRAAIDHIVVSRSLAGRLVPGSMRRVTYATAD
                     VRRFRLSDHCPVAVTLELP"
     gene            complement(1105..1869)
                     /locus_tag="VD791_13485"
     CDS             complement(1105..1869)
                     /locus_tag="VD791_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV09040.1"
                     /translation="MNAFRLDGKVAVVTGSSRGIGRSIAEHMAAYGAKVVISSRKQPA
                     CEEVAAAIRAAGGDAIAVPANIGEKAQLEALVAAARRQWGRIDVLVCNAASNPYFGPM
                     AGLPDEAFHKILQNNVVSNLWLANMVLPEMAQRKDGVVIIVSSIGGLKGTALLGAYAV
                     SKAADMQLARNLAVEWGKHNVRVNCIAPGLVKTDFARALWENPELLARNEAAAPLRRI
                     GEPDDIGGIAVFLASRAAAFITGQTIVADGGITIAS"
     gene            1995..2252
                     /locus_tag="VD791_13490"
     CDS             1995..2252
                     /locus_tag="VD791_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV09041.1"
                     /translation="MDLDKTFEFTTNLDRRAIEARLEEVREAADGLLLADVAQALTGV
                     AGKSREEMAQRIRTALDALEGSGQKRMIALLELAEINLANL"
     gene            complement(2277..3392)
                     /locus_tag="VD791_13495"
     CDS             complement(2277..3392)
                     /locus_tag="VD791_13495"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXV09042.1"
                     /translation="MDEKLVYNVNVSAQDVLLTPTEIKQRVPLTEQAEETVLRGRNTV
                     ERILDRKDHRLMVVVGPCSIHDPQAAMDYARRLKRLADEVSDTLQLIMRVYFEKPRTT
                     TGWKGLINDPHMDDSFDIAQGLETARKVLRDINELGLPAGTEALDPISPQYLGDLVAW
                     SAIGARTTESQTHREMASGLSTPVGFKNGTDGGLQVAINALVSVSSAHSFLGINQSGQ
                     VAVIRTKGNRYGHIVLRGGSKGPNYDSVTISLVEKELAKHKLAANIMVDCSHANSNKD
                     PALQPLVMNDVVHQVIEGNRSIVGVMLESNLHAGNQSIPADPSQLKYGVSVTDACIDW
                     GTTEKVLREARLKLKDALPARLPRLADVRSLRGGVVF"
BASE COUNT          483 a         1175 c         1222 g          567 t
ORIGIN      
        1 ctttgtgcgc attaaagcgc gtcttaagaa cctttccccg cctctaacga ggcggggtgg
       61 cgctcccctc ctggcaagga ggggtggcgc cgaaggcgac ggggtggtcg gcgccggggc
      121 ggtgtggtgt actacggcag ctccagcgtc accgccaccg ggcagtgatc cgacagccgg
      181 aaccttcgca cgtcggccgt ggcgtaggtc acgcggcgca tcgatcccgg cactagccgg
      241 cccgccagcg aacggctgac cacgatgtgg tcgatcgccg cgcggcgcga ggcctgcgcg
      301 aagcagcgcg cgtcgcggcg cacgggcgtc gcgtcggtga ggtcggcctc gggcgggctg
      361 ccgtcgtcga gctcgggcca catcgcggtc agcctgccga ggcggtcgcg cgcctggcgc
      421 cgctccgagg agaagcggcg gttgaagtcg cccgccactg caaacggggc gccgtcgcgc
      481 gctcgtgcgt cgatccagcg ttccagcacc ggcacctgct cggcgagcgt gcggcagtcg
      541 ccgcgctcgc tgctgagccg ctcgccgtgg cagcccgatt tcaggtgcac cgccagcacc
      601 cgcacctcgc gttgcgatcc ggggaacagc gtcaggtccg cgccccagcg cacgcggctt
      661 ccctccagcc ccaggtcggg atagtccgcg cagcggaacg gcacgccggg acggatcgcg
      721 aagccgacgt tctggacgtg caccctgcgc gtgaagcaga agtcgtggcc ggggaacacc
      781 aggcgcgcgg cctgcggtcc gtccacttcc tgcagcgcga cgacgtcggc atcgagccgg
      841 cgggcgtact cgcgcagctt ggcgaaatcc gcggccgtgc gccgcatggc acgctcgccc
      901 gcggcgatgt cgcacggaat ccagcgccgt tcgccggacg gcggttcgtc gcggcaggat
      961 gccgctaggc gctcgaaggt ctcgggcgcg atcagccact ggagattcca ggttgccaac
     1021 cgaaaggtgc gcggctgcgc gcctgcgtgc ggcgcgcaca gcagtgcgag gcaggcgacg
     1081 gcggccgcga gccggcgccg cctatcagct ggcaatggtg atgccgccgt cggcgacgat
     1141 ggtctggccg gtgatgaagg ccgcggcgcg cgaagccagg aacacggcga tgccgccgat
     1201 gtcgtcgggc tcaccgatgc gccgcagcgg ggcggcggct tcgttgcgcg ccagcagctc
     1261 ggggttctcc cacagggcgc gggcgaagtc ggtcttcacc aggcccggcg cgatgcagtt
     1321 gacgcggacg ttgtgcttgc cccactccac ggcgaggttg cgggcgagct gcatgtcggc
     1381 cgccttggat acggcgtagg cgcccagcag ggcggtgccc ttcagcccgc cgatcgagga
     1441 cacgatgatg accacgccgt ctttgcgctg cgccatctcc ggcagcacca tgttcgccag
     1501 ccacaggttg ctgacgacgt tgttctgcag gatcttgtgg aacgcctcgt ccggcaggcc
     1561 ggccatcggg ccgaagtagg gattggaggc ggcgttgcac accagcacgt cgatgcgtcc
     1621 ccactgccgg cgcgcggccg cgaccagcgc ctcgagctgg gccttctcgc cgatgttggc
     1681 cggcaccgcg atggcgtccc cgccggcggc gcggatcgcg gcggccacct cctcgcaggc
     1741 cggctgcttg cggctggaga tcaccacctt cgcgccgtag gcggccatgt gctcggcgat
     1801 cgatcggccg atgccgcggc tcgagccggt gacgacggca accttgccgt ccaggcggaa
     1861 ggcgttcatg ggtcggctcc ggttggtggg atcggttcct gggcgatttg acgttagttg
     1921 acgcgcgacc ctgcatttta tggcatttgt gccgtcatac acccttggga cttggccctg
     1981 cgacggggag cgacatggat ctggacaaga ccttcgagtt caccaccaac ctcgaccgcc
     2041 gcgcaatcga ggccaggctg gaggaggtcc gcgaggccgc ggacggactg ctgctggccg
     2101 atgtcgcgca ggcgctcacc ggggtcgccg gcaagagccg cgaggagatg gcgcaacgca
     2161 tccgcaccgc gctggacgcg ctggagggct cgggccagaa gcgcatgatc gcgctgctcg
     2221 agctggccga gatcaacctc gcgaatctgt gacctgatgc gcccccaagg cgcgtcttaa
     2281 aagacaaccc cgccgcgcag gctacgcacg tcggccaatc gcggcaggcg ggcggggagc
     2341 gcgtccttga gcttcaggcg cgcctcgcgc agcaccttct cggtggtgcc ccagtcgatg
     2401 caggcgtcgg tcaccgacac gccgtacttg agctgcgacg gatcggcggg aatcgactgg
     2461 ttgcccgcgt gcagattgct ctccagcatc acgcccacga tcgaccggtt gccctcgatc
     2521 acctggtgca ccacgtcgtt catcaccagc ggctgcagcg cgggatcctt gttggagttc
     2581 gcgtggctgc agtcgaccat gatgtttgcc gccagcttgt gcttggccag ctccttctcc
     2641 accagcgaga tggtcaccga gtcgtagttc ggacccttgc tgcccccgcg caggacgatg
     2701 tggccgtagc ggttgccctt ggtgcggatc accgccacct gcccggactg gttgatgccg
     2761 aggaagctgt gcgcactgga caccgacacc agcgcgttga tcgccacctg caggccgccg
     2821 tcggtgccgt tcttgaatcc caccggggtg gacaggccgc tcgccatctc gcgatgcgtc
     2881 tgcgattcgg tggtgcgcgc gccgatggcg ctccacgcca ccagatcgcc caggtactgc
     2941 gggctgatcg gatcgagcgc ctcggtgccg gcgggcagcc ccagctcgtt gatgtcgcgc
     3001 agcaccttgc gcgcggtctc gagcccctgc gcgatgtcga agctgtcgtc catgtgcggg
     3061 tcgttgatca gacctttcca gccggtggtg gtgcgcggct tctcgaagta cacgcgcatg
     3121 atcagctgca gcgtatcgga cacttcgtcc gccaggcgct tgaggcgccg tgcgtagtcc
     3181 atcgcggcct gcggatcgtg gatcgagcag gggcccacca ccaccatcag ccgatggtcc
     3241 ttgcggtcga ggatgcgctc cacggtgttg cgcccgcgca gcaccgtctc ctcggcctgc
     3301 tcggtgagcg ggacccgctg cttgatctcg gtgggggtga ggaggacgtc ctgcgcgctg
     3361 acgttgacgt tgtagaccag cttctcgtcc atggtgtttc cgtgctcggg gggctcttct
     3421 gcctgcgggg gttgcccggc gcgtccg
//
LOCUS       DASPGE010000410         3083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_813657, whole genome shotgun sequence.
ACCESSION   DASPGE010000410 DASPGE010000000
VERSION     DASPGE010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813657"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VD791_13500"
     CDS             <1..829
                     /locus_tag="VD791_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-hydroxynicotinate reductase"
                     /protein_id="HXV09043.1"
                     /translation="VVNGVEEHRMRVGCGSATIGIFAKQWHGHVDEVVVVDDHITGVL
                     TEHQAGRFLDMPPSGIKIKGRKSTPGRYFQVANPGTGWGGTDITEPLGIVTGFDPKVA
                     RPGERILMVSTTGEDAAYFVLDEQLRPAPAPMPDAVRKVVERIGENCEPALTTVLFLA
                     GAGGSLRAGVTENPVRLTRSIKDLLVRVTCGGAPVYVWPGGGITVMVDVTRMPDNSFG
                     WVPTPAIVAPIEFSMRLADYAALGGHMDRVRTVDDVLRQERVRVVGWDGINPWPVGT"
     gene            826..1680
                     /locus_tag="VD791_13505"
     CDS             826..1680
                     /locus_tag="VD791_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HXV09044.1"
                     /translation="MRCAQRAPLPDGRWHFQHGPIDLIIGADGDAGSVSQALETAWRR
                     FDAVLGELLGELRLLRSPVQEAQGVRGSVARRMLAACWPHRERFITPMAAVAGAVADE
                     LIECFGAQPGVRRAYVNNGGDIALHLAPEECYRVGLFADLGRIVRDKPLHLDGRFEVT
                     AALPVRGVATSGWRGRSFSLGIADSVTVLARTAAQADAAATMIANAVTVDDDAIVRRP
                     ANTLKDDTDLGDRPVTVEVGTLSFGRVACALEAGAGHAHGLLERGVIWAAWLWLQGQA
                     RSVGGSRT"
     gene            1677..2261
                     /locus_tag="VD791_13510"
     CDS             1677..2261
                     /locus_tag="VD791_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HXV09045.1"
                     /translation="MKAKIRKLVVQVEEVRSEMGREVDPPGRKAVAMAVIENPCAGRY
                     VEDLNELVEIGEELGGLLGERCVRALGIAPHQTESYGKAAIVGEAGELEHAAAILHPK
                     LGATLRKALEKGAALVPSAKKIGTLGAAVDVPLGHKDAAYVRSHFDAVEARVSDAPRA
                     REIVVAVAVTDSGRPLARVGGLQKHEIKGQDGLR"
     gene            2303..>3083
                     /locus_tag="VD791_13515"
     CDS             2303..>3083
                     /locus_tag="VD791_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXV09046.1"
                     /translation="MDAAVARKPWLKSYPPGVPEHMPALEFESIPRMFEASVARYREQ
                     PAFESFGVRITYADLDRLSRDFAAYLQSKLGLRKGDRVAVMMPNILQYPVAVFGILRA
                     GLVVVSVNPLYTARELQHQLADSGATAIVIFESAAHTLQEIIARTPVRHVVVASVGDM
                     LGMLKGNLINFVFRKVKKMVKPWSIPGAVRFPEALAQGGARSLSTPAIAADDLAFLQY
                     TGGTTGVSKGAMLQHRHILANVQQILTYTGDTVRDSREIAIA"
BASE COUNT          468 a         1017 c         1154 g          444 t
ORIGIN      
        1 ggtggtcaac ggcgtcgagg agcatcgcat gcgcgtgggt tgcggctcgg cgaccatcgg
       61 catcttcgcc aagcaatggc acgggcacgt ggacgaggtg gtggtggtgg acgaccacat
      121 caccggcgtg ctcaccgagc atcaggccgg ccgcttcctc gacatgccgc cgtcgggcat
      181 caagatcaaa ggacgcaagt cgaccccggg gcgctacttc caagtcgcca atcccggcac
      241 cggctggggc ggcaccgaca tcaccgagcc gctcggcatc gtcaccggct tcgatccgaa
      301 ggtcgcccgc cccggggagc gcatcctgat ggtctcgacc accggcgagg acgccgccta
      361 cttcgtgctc gacgaacagc tgcgcccggc gccggcgccg atgccggatg cggtgcgcaa
      421 ggtggtggag cgcatcggcg agaactgcga gccggccctg accacggtgc tgttcctggc
      481 cggcgccggc ggcagcctgc gcgccggcgt gaccgagaac ccggtgcggc tgacgcgttc
      541 gatcaaggac ctgctggtgc gcgtgacctg cggcggggcg ccggtgtacg tgtggcccgg
      601 cggcgggatc accgtgatgg tggacgtcac gcgcatgccg gacaactcct tcggctgggt
      661 gcccaccccg gccatcgtcg cgccgatcga gttctcgatg cgcctggcgg attacgctgc
      721 gctcggcggc catatggacc gcgtgcgcac cgtcgacgac gtgctgcgcc aggagcgcgt
      781 gcgcgtggtg ggctgggacg gcatcaaccc gtggccagtg gggacgtgag gtgcgcgcaa
      841 cgcgcgccgc tgcccgacgg acggtggcac ttccagcacg gtcccatcga tctgatcatc
      901 ggtgccgacg gcgacgcggg ctcggtgtcg caggcgctgg aaacggcgtg gcggcgcttc
      961 gacgccgtcc tcggagagct actcggcgag ctgcgcctgt tgcgctctcc ggtccaggag
     1021 gcgcagggcg tgcgcggcag cgtcgcgcgc cgcatgctgg ccgcctgctg gccgcaccgc
     1081 gagcgcttca tcacgcccat ggccgcggtg gccggcgcgg tggcggatga gctgatcgag
     1141 tgcttcgggg cgcaaccggg tgtgcgccgc gcgtacgtca acaacggcgg cgacatcgcc
     1201 ctgcacctcg cgcccgagga gtgctatcga gtcggcctgt tcgccgacct cggccgcatc
     1261 gtgcgggaca aaccgctcca cctcgacgga cggttcgagg tgacggcggc gctgccggtg
     1321 cgtggcgtgg ccaccagcgg ctggcgcggc cgcagcttca gcctcggcat cgccgacagc
     1381 gtcacggtgc tggcgcgcac cgccgcgcaa gccgacgcgg ccgccaccat gatcgccaac
     1441 gcggtcaccg tggacgacga cgccatcgtg cgccggccgg cgaacaccct caaggacgac
     1501 accgatctcg gcgaccggcc ggttaccgtg gaagttggta ccctatcgtt cgggcgcgta
     1561 gcttgcgcgc tggaggcagg cgccggccac gcccacggtc tgctggagcg tggggtgatc
     1621 tgggcggcgt ggctgtggct gcaaggacag gcccgcagcg tcggtgggag caggacatga
     1681 aggcgaagat caggaagctg gtggtgcagg tggaagaggt gcgcagtgag atgggccgcg
     1741 aggtcgatcc gccgggtcgc aaggcggtgg ccatggcggt gatcgagaac ccctgcgccg
     1801 gccgctacgt cgaggacttg aacgagctgg tcgagatcgg agaggagctg ggcggcctgc
     1861 tcggcgagcg ctgcgtgcgg gcgctgggca tcgccccgca ccagacggag agctacggca
     1921 aggccgccat cgtcggcgag gcgggcgagc tggagcacgc cgcggcgatc ctgcacccga
     1981 agctgggcgc gaccctgcgc aaagcgctgg agaagggcgc cgcgctggtg ccttctgcga
     2041 agaagatcgg caccctgggt gcggcggtgg acgtgccgct cgggcacaag gacgcggcct
     2101 acgtgcgcag ccacttcgac gccgtggagg cgcgcgtgag cgacgcgccg cgcgcgcgag
     2161 agatcgtggt cgcggtggcg gtgacggaca gcggccggcc gctggcgcgc gtgggcggtt
     2221 tgcagaagca cgagatcaaa ggacaggacg ggctgcgcta gcccggttat ttcatcagga
     2281 cgggaccggc aggaggacga cgatggatgc ggctgtggcg cgcaagccct ggctgaagag
     2341 ctacccgccg ggtgtgcccg agcacatgcc ggcgctggag ttcgagtcga tcccgcgcat
     2401 gttcgaggcg agcgtggcgc gttaccgcga gcagccggcg ttcgagagct tcggcgtgcg
     2461 tatcacctac gccgacctgg accggttgtc gcgcgatttc gccgcctacc tgcagtccaa
     2521 gctcggactc aggaaggggg accgggtggc ggtgatgatg cccaacatcc tgcagtaccc
     2581 ggtggcggtg ttcggcatcc tgcgcgccgg gctggtggtg gtcagcgtca acccgctgta
     2641 cacggcgcgc gagctgcagc accagctcgc cgattcgggc gcgaccgcca tcgtgatctt
     2701 cgagagcgct gcgcacacgc tgcaggagat catcgcgcgc accccggtgc ggcatgtggt
     2761 cgtggcttcc gtgggcgaca tgctgggcat gctcaagggc aacctgatca acttcgtgtt
     2821 ccgcaaggtc aagaagatgg tgaagccgtg gagcattccg ggcgcggtgc gctttcccga
     2881 ggcgctggcc cagggcgggg cgcgctccct gagcacgccc gccatcgcgg ccgacgacct
     2941 cgccttcctg cagtacaccg gcggcaccac aggcgtgtcc aagggtgcga tgctgcagca
     3001 tcgccacatt ctcgccaacg tgcagcagat actgacctac accggcgaca ccgtgcgcga
     3061 cagccgggag atcgccatcg cgc
//
LOCUS       DASPGE010000411         9779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_110984, whole genome shotgun sequence.
ACCESSION   DASPGE010000411 DASPGE010000000
VERSION     DASPGE010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9779
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110984"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..1486)
                     /locus_tag="VD791_13520"
     CDS             complement(254..1486)
                     /locus_tag="VD791_13520"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXV09047.1"
                     /translation="MKLGFIGAGFVARFQAVAIEQVRGLEIAGLVRRRGSDALAAFCR
                     GHGLGPAAIYDSVGELANRVDALAILVPNFCRIEVMEQIAAAVKAGAALKGVIVEKPL
                     GRNMREARRMVELAREARLRTAYFENQIFMRAIQTQRTQLLPVMRNMGPPTLTRSAEE
                     HGGPHEPWFWDPTRQGGGVLLDMGCHSIAVGRYALTPPGKDLLFLQPLAVTADLGLLK
                     WGQPRWREKLLKDRGVDYSKAPAEDFATGMITYRNPETKQIVKAQFTDSWMFEKQGLR
                     LFMDGMGPGYAFEINTLVSPLNVFIGDAAAQAIADQESALEKATASRGLLAVQYNEAD
                     LYGYTDENRDMLQAFGAGRDAMLSWEFGLEVQRLMMASYLSAERRKSVDLTDPATLAE
                     LETYVPAIQQGKGHDQLC"
     gene            1667..2698
                     /locus_tag="VD791_13525"
     CDS             1667..2698
                     /locus_tag="VD791_13525"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV09048.1"
                     /translation="MHKSLLAACCAAVLATGGIAPASAKDSFTIGLSNGWVGSEWRTQ
                     MIDEAEQAAKEWAKRGVTVKTIAQSSNVDVPGQIAQVRNFINQGVDAIIVNPNSPTAF
                     DPIFAQAKAKGILVISTDAEVSSKDAIYVGIDQKGWAMQSAEWLAKTLKGKGNVVAIN
                     GVAGHPANQMRVAGYREVFAKHPGIKILNEVNANWDEAQGQQAMQNLLATYPQINGVW
                     VQDGMAAGAWRALTDAKRSDVAATGEIRVDFLKTWKEKKFNSGASVNPPGCMASALNV
                     AVLRLQGRQLKDGIFKGQYANALYIPIPLIDSSNFERIYEQWKDKAGYISVSTVVTPE
                     QAEQMFFKK"
     gene            2728..4230
                     /locus_tag="VD791_13530"
     CDS             2728..4230
                     /locus_tag="VD791_13530"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HXV09049.1"
                     /translation="MPVLEASGVRKQFGGVVALAGAQFALEAGEVHALIGSNGCGKST
                     LCKIIAGSVAADAGSLLLDGRPVSFAGPRQAAAAGIGVFYQELSVIREMTVAENIFLG
                     REPRNRAGMLDREALEAAAGAALAQFGDVLGAGVHPRTRVAELSADQSQLVEILKVLA
                     ANPRILIFDEATAALDRNQVTAVFQRIRTLKAEGRSVIFITHRMDEVFEIADRVTVMR
                     NGATVLTCASAQTTRDALVGAMVGGAVARAGRDRRHVPSEAVALEVENLSSAKLAGVS
                     FKLRRGEIVGLGGLHGQGQSELLRALFGAMPLHGGAVRVAGEPLDPRGPRAVMRRSIA
                     YVSGDRGRYGVMAIRPIFENLVISLLARDRRWTVARQRLEREVGPFIERLKLKFSSLQ
                     AAVSELSGGNQQKVVLGRWLATRPSVLLLDDPTKGIDIQTKEDLYATMDELCRQGVAI
                     LLHSSDDEELLAVADRVLVFNGGQVMGELSGEQRTRFALYQAAYASKHPA"
     gene            4235..5200
                     /locus_tag="VD791_13535"
     CDS             4235..5200
                     /locus_tag="VD791_13535"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV09050.1"
                     /translation="MPAAAASRFSTQRHPYLMAAVVFLVLCVVNAVLQPTFLRFGVAA
                     SNVAAFLPMALVAVGQTYVVLASDIDLSAGAIVSLVNVVVVSIIDGMAGSGTSMGLGL
                     AAGLALGLTCGVVNGLCVAYLRFQPIVTTFATGIVFAGLALWVLPEAGKQVPPALYLT
                     YAGSVLGVPTVVWILAAAIAFAAIVGRTRFYQALRATGGNMQAAYQTGVPVARVRLGA
                     YAISGLFAALAAFALVGETASGDPLIGGALTLSSVTAVVLGGTALRGCVGGVAGSILG
                     AFILGLISNVIFFAQLPFEWQGLIQGLIILTALAGGVVMARGRQD"
     gene            5202..6164
                     /locus_tag="VD791_13540"
     CDS             5202..6164
                     /locus_tag="VD791_13540"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV09051.1"
                     /translation="MPAPRLVALLKQPLSIAVVAILALLAAGELLSPGFASPRQIVTL
                     LTIAALLGVVAAGQNLVILGGREGIDLSVGGIISFAAFLAGNIMQQQDAYLVQALVIV
                     LCVSFLIGLTTGLGVTWLRIPPLVMTLGMLGVVQGGLVLLTRGVPSGRAAPTLSRIVS
                     EPMLFGLPGILFIWILVGVALTLMLRRTTFGFNVYAIGTNEPAAALAGVPVRRTRALL
                     FALSGLLSGLTGILLLGYTEYVFVGAGDQYMLPSIIAVVIGGTSLAGGSGGYVGTMAG
                     AIVLVLLQSILTTLNIPPFGRQIVFGVTLLGLMLLYGRQRRLRG"
     gene            complement(6189..7250)
                     /locus_tag="VD791_13545"
     CDS             complement(6189..7250)
                     /locus_tag="VD791_13545"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HXV09052.1"
                     /translation="MKRSAARCICCWVLLLIAGAAWPQDLDRQKAGVVKVTSRLDGAR
                     RTGTGFIVHLDADAAYIVTASHVVEGDNRPLIEFFTRRNATVEAETARIEGGDPRGLA
                     LLVVRGKDRLPAGIGALPLRRGADLRGGESVTTIGFPRGGGAWAVVHATVVSREGRDL
                     MLDGSIDEGNSGGPVLMGDNVVGVVTLLDGKFARAVPADLAGFVLEGWGVRPDAARAA
                     KGAPAPPAARTEAPRAEQTSRPDASVEITRVLCERLRAGTSFRVTFNGQAQGPAGSAV
                     RTALLRAGEEVSTPKTACQGWNGCRRGTSDPGGTRWSVSVIALTPVPTHVTVAVMPEG
                     AAPATPYATQRVELDCPRF"
     gene            complement(7247..8839)
                     /locus_tag="VD791_13550"
     CDS             complement(7247..8839)
                     /locus_tag="VD791_13550"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HXV09053.1"
                     /translation="MVRVFISYASEDRPGAERLAQALAQSGFAVWWDRKIPLGSSFDQ
                     VIEENLAQAECVLVLWTAASVQSRWVRAEASEAAAREVLVPVLMEPELKLPLEFRLLQ
                     AANLSDWSGDAQHAEFSALLAHITAIGGRAGAAQHDPGAAGAHAGMQPAARAHRPPGP
                     LRARDSATQRGPRRPGLALMLLAPGVAILAAAVAATMWRVPTRLQLDLVVDRLAFTLA
                     GERGVDMPVRPLGFRTLSVENFDRLTFTPDRVRWIAPGSSRMPPRSATVILNSPADAA
                     AVAVVEGVGRGNSTAGAGRMQPLAIAPRSRVILATSADASNRVTLRVEGQPLDTNILP
                     AAELALSATNVTLGESSPSAPADGPVALAFSLPEDRPFVQVQGTRRWFAITATLSASA
                     ALALLDRAGVNGVELLKQGGGGTVESAVIANGAIRYPQQPRIAPVPVGARDFVTLGDM
                     SRTTITRLELSAEQSSMLLTMDGVATQATLASGGAERSLCITALQSLWHRSRVGVALL
                     AAVCLASMLAGGYRLYEEHAAA"
     gene            complement(8848..9537)
                     /locus_tag="VD791_13555"
     CDS             complement(8848..9537)
                     /locus_tag="VD791_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV09054.1"
                     /translation="MQAQTQLQYQKRANHYEGVRARPVSGFDIELLAAQAAYADDAEK
                     IGERFHVRFFLTRPRAVHLVVRELDYRYFYWLDRVQPESPWRAGFANVFSWSTAEVVR
                     QLGDLRISDLGVVVRLDRATPSAIEEVAPALFYQSQFPAAVRGYVFHFRLREEAMLRA
                     AIFKDTGTEPIWSAPSVRRTAGRPFSYRWDLAEGGPPEGAYRLVLSGYLLSTNDPISQ
                     VVRFHHRPQVR"
BASE COUNT         1443 a         3412 c         3390 g         1534 t
ORIGIN      
        1 tacgcccgac cgcacgcgag gtgatcgcgt ttctcgatcg ggtcagagcc gatcccaggt
       61 ttcgcacgac gttcgcggag ggcggcagtg cagaaggcat ctcggtgagt tgccgggtcg
      121 cgggttgatg gcgctgtacc gccgacgctg gccgccggcg cgtagctcgg atcgtgccgc
      181 gttgtgcgcg gcctgcgcta cgggcaacca agcggcatcc cgatcgccgc gctagccccg
      241 acagggtacc gagctagcac agctgatcgt gccccttgcc ctgctggatt gcgggcacgt
      301 aggtttccag ctcggccagc gttgcgggat cggtcaggtc gacgctcttg cggcgctcgg
      361 ctgacaggta gctcgccatc atcagccgct gaacctcgag gccgaactcc caggacagca
      421 tcgcgtcacg gccggcgccg aatgcctgca gcatgtcgcg gttctcgtcg gtgtagccgt
      481 agagatccgc ctcgttgtac tgcacggcca gcagcccccg gctcgcggtc gccttctcca
      541 gcgcgctctc ctggtcggcg atcgcctgcg ccgccgcgtc gccgatgaac acgttgagtg
      601 gcgacaccag ggtgttgatc tcgaacgcgt agccggggcc catgccgtcc atgaacaggc
      661 gcaggccctg tttctcgaac atccacgagt cggtgaactg ggccttcacg atctgcttcg
      721 tctccgggtt gcggtaggtg atcatgccgg tggcgaagtc ctccgccggc gccttgctgt
      781 agtccacgcc tcggtccttg agcagctttt cgcgccaccg gggctgcccc cacttgagca
      841 gaccgagatc ggcggtgacc gccagcggct gcaggaacag cagatccttg ccgggcggcg
      901 tcagggcgta gcggcccacc gcgatgctgt ggcagcccat gtcgagcagc acgccgccgc
      961 cctgccgggt gggatcccag aaccacggct cgtgcgggcc gccgtgctcc tcggccgagc
     1021 gcgtcagcgt tggcgggccc atgttgcgca tgaccggcag cagctgcgtg cgctgggtct
     1081 ggatcgcgcg catgaagatc tggttctcga agtaggcggt gcgcagcctt gcttcgcgcg
     1141 ccagctccac catccgccgc gcttcgcgca tgttgcgccc gagcggtttc tccacgatga
     1201 cgcccttcag cgccgcaccg gccttcaccg ccgcagcgat ctgctccatc acctcgatgc
     1261 ggcagaaatt cggcaccagg atggcgagcg cgtccacccg gttcgccagc tcgccgaccg
     1321 aatcgtagat cgccgccggc cccagtccat ggccgcggca gaacgccgcc agtgcatcgc
     1381 tcccgcgccg ccgcaccagg cccgcgatct ccaggccgcg cacttgctcg atcgccaccg
     1441 cctggaagcg ggcgacgaag cccgccccga tgaatccgag tttcatgtgg ttacccctgt
     1501 tcccgacgcg ttgcatcggc gatcccggca cgcagcgtgc gcggtcgccg gcgacgcggt
     1561 cattgtaggt cagatcgcgg acaatccttt acactgcgcc tcgcaagccg gcggcgggcc
     1621 cgcccgcacg tcgaaaccaa gaacgtccat ccctggagga gacaccatgc acaagtccct
     1681 gttggcggcc tgctgcgccg ccgtcctcgc gaccggcggc atcgcgccgg cgagcgccaa
     1741 ggacagcttc accatcgggt tgagcaacgg ctgggtcggc agcgaatggc gcacgcagat
     1801 gatcgacgag gccgaacagg ccgcgaagga atgggccaag cgcggcgtca cagtcaagac
     1861 catcgcgcag agctccaacg tggatgtgcc cggccagatc gcgcaggtgc gcaacttcat
     1921 caatcagggg gtggacgcca tcatcgtcaa cccgaacagc cccacggcct tcgacccgat
     1981 cttcgcgcag gccaaggcca agggcatcct ggtcatttcc accgacgccg aggtgtcgtc
     2041 gaaggacgcg atctacgtcg gcatcgacca gaaggggtgg gccatgcagt ccgccgagtg
     2101 gctggcgaaa accctcaagg gcaagggcaa cgtggtggcg atcaacggcg tggccggcca
     2161 tccggccaac cagatgcggg tggcgggtta ccgcgaggtg ttcgccaagc atcccggcat
     2221 caagatcctg aacgaggtga acgcgaactg ggacgaggcg caaggtcagc aggcgatgca
     2281 gaacctgctc gccacctatc cgcagattaa cggggtgtgg gtccaggacg ggatggcggc
     2341 cggcgcctgg cgcgccctga cggacgcgaa gcgcagcgac gtcgcggcca cgggcgagat
     2401 ccgcgtcgac ttcctcaaga cctggaagga gaagaagttc aacagcggcg cttcggtgaa
     2461 cccgcccgga tgcatggcga gcgcgctcaa cgtcgcggtt ctgcgcctgc aggggcgcca
     2521 gctcaaggac ggtatcttca agggacagta cgccaacgcc ctgtacattc ccattccgct
     2581 gatcgacagc tccaacttcg agaggatcta cgagcagtgg aaggacaagg ccggctacat
     2641 ctcggtgagc accgtggtca cgcccgagca ggccgagcag atgttcttca agaagtaggt
     2701 ccgcgccgcc gcgctcacag cgcgcccatg cccgtcctgg aggcgagcgg cgtccgcaag
     2761 cagttcggag gcgtggtggc gctcgccggc gcgcagttcg cgctcgaggc cggcgaggtg
     2821 cacgcgctga tcggctcgaa cggctgcggc aagagcacgc tctgcaagat catcgccggc
     2881 tccgtggccg cggacgccgg cagcctgctc ctggacggcc gaccggtgtc gttcgccggt
     2941 ccgcgccagg ccgcggcggc cggcatcggc gtgttctatc aggagttgag cgtcatccgc
     3001 gagatgacgg tggccgagaa catctttctc ggccgcgagc cgcgcaaccg cgccggcatg
     3061 ctggatcgcg aggcgctcga ggctgcggcc ggcgcggcgc tcgcgcagtt cggcgacgtg
     3121 ctcggtgccg gcgtgcaccc gcggacgcgc gtggccgagc tgtcggccga ccagagccag
     3181 ctggtggaga tcctcaaggt tctggccgcg aacccgcgca tcctcatctt cgacgaagcc
     3241 accgcagcgc tggaccgcaa ccaggtgacc gcggtgttcc aacgcatccg cacgctgaag
     3301 gccgagggcc gctcggtgat cttcatcacg caccgcatgg acgaggtgtt cgagatcgcc
     3361 gatcgcgtca cggtcatgcg caacggcgcc acggtgctca cctgtgcgtc ggcgcaaacc
     3421 acgcgtgacg cgttggtcgg cgccatggtg ggcggcgcgg tggcgcgcgc aggccgcgac
     3481 cggcggcacg tgccgtcgga ggcggtcgcg ttggaggtgg agaatctgtc ctccgccaag
     3541 ctggcgggcg tatcgttcaa gctgcgccgg ggcgaaatcg tggggctcgg cgggctgcac
     3601 gggcagggac aatccgagct gctgcgcgcg ctgttcgggg cgatgccgct gcacggcggg
     3661 gcggtccgcg tcgccggcga gcctctggac cccaggggcc cgcgggccgt catgcggcgc
     3721 tccatcgctt atgtctcggg ggaccgcggg cgctacggcg tgatggcgat tcggccgatc
     3781 ttcgagaatc tggtgatctc gctgctcgcc cgggaccgcc gctggacggt ggcgcggcag
     3841 cgcttggagc gggaggtcgg accgttcatc gagcggctca agctgaagtt ctcctcgctg
     3901 caggccgccg tgtcggagct gagcggcggc aatcagcaga aggtggtgct cggccgctgg
     3961 ctggcgacgc gccccagcgt gctgctgctg gacgatccga ccaagggcat cgacatccag
     4021 accaaggagg acctgtacgc gaccatggat gagctgtgcc gccagggcgt ggcgattctg
     4081 ctgcattcct ccgacgacga agagctgctc gccgtggccg accgcgtgct ggtgttcaac
     4141 ggcgggcagg tgatgggtga gctgagcggc gagcaacgca cgcggttcgc gctgtaccag
     4201 gccgcctacg cctcgaaaca cccggcctga gagcatgccc gcggccgcag cttcacgctt
     4261 ctcgacgcag cgccatccct atctgatggc ggcggtcgtg ttcctggtgc tgtgtgtggt
     4321 gaacgcggtc ctgcagccca ccttcctgcg cttcggcgtg gccgcctcga acgtcgcggc
     4381 gttcttgccg atggcgctgg tcgcggtcgg ccagacctat gtcgtgctgg cctccgacat
     4441 cgatctgtcg gccggcgcca tcgtttcgct ggtcaacgtc gtggtcgtgt ccatcatcga
     4501 cggcatggcc ggcagcggca cgagcatggg gctgggcctg gccgccgggc tggcgctggg
     4561 attgacctgc ggtgtggtca acggcctgtg cgtggcctac ctgcgcttcc agccgatcgt
     4621 caccacgttc gccaccggca tcgtgttcgc gggtcttgcg ctgtgggtgc tgccggaggc
     4681 cgggaagcag gttccgccgg cgctgtacct gacctacgcc ggctcggtgc tgggcgtgcc
     4741 caccgtggtg tggatcctgg ccgcggcgat cgccttcgcg gcgatcgttg gccggacgcg
     4801 cttctaccag gcgctgcgtg cgacgggcgg caacatgcaa gcggcctatc agaccggcgt
     4861 gccggtcgcg cgcgtgcggc tcggcgcgta cgccatctcg ggcttgttcg cggcgctggc
     4921 ggcgttcgcg ctggtcggcg agacggcctc gggcgatccg ctgatcggcg gcgcgctgac
     4981 gttgtcgtcg gtgaccgcgg tggtgctggg cggcacggcg ctgcgcggct gcgtcggcgg
     5041 cgtggcgggc agcatcctgg gggcgttcat cctcgggctg atcagcaacg tcatcttctt
     5101 cgcgcagctg ccgttcgaat ggcaggggtt gatccagggg ttgatcatcc tgaccgctct
     5161 ggcgggcggg gtggtgatgg cgcgcggcag gcaggattga catgccggcg cccaggctgg
     5221 tcgcgctcct gaagcagccg ctgtcgatcg ccgtcgtcgc gatcctggca ctgcttgccg
     5281 ccggcgagct gctgtcgccg ggcttcgcct cgccgcggca gatcgtcacc ctgctcacca
     5341 tcgccgccct gctcggcgtg gtggccgccg gccagaacct ggtgattctc ggcgggcgcg
     5401 agggcatcga cttgtcggtc ggcggcatca tttcgttcgc cgcgttcctc gccggcaaca
     5461 tcatgcagca gcaggacgcc tatctcgtgc aggcgctcgt gatcgtcctg tgcgtcagct
     5521 tcctgatcgg gcttaccacg ggcctcggcg tcacgtggct tcgcattcca cctctggtga
     5581 tgacgctggg catgctcggc gtggtgcaag gcgggctggt cctgctcacg cgcggtgtgc
     5641 cgtccggccg cgccgcgccg acgctgagcc ggatcgtcag cgagccgatg ctgttcgggc
     5701 tgcccgggat cctgttcatc tggatcctgg tcggcgtggc gctcactctg atgctgcgcc
     5761 ggacgacctt cggcttcaac gtgtacgcca ttggaaccaa cgagcccgcc gcggcgctcg
     5821 cgggcgtccc ggtccggcgc acgcgcgcgc tgctgttcgc gctgagcgga ctgttgagtg
     5881 gcctcaccgg catcctgttg ctgggctaca ccgagtacgt gttcgtcggc gccggcgatc
     5941 aatacatgct gccgtccatc atcgccgtgg tcatcggcgg cacatcgctc gcgggcggca
     6001 gcggtggata cgtcggaacc atggcgggag cgatcgtgct ggtcctgctg caaagcattc
     6061 tcaccacgtt gaacatcccg cccttcggcc gccagatcgt gttcggcgtc acgctgctcg
     6121 ggctgatgct cctgtacggg cgtcaacgcc ggctgcgcgg ctagctgcgc ggccgctcgc
     6181 gcggcgcttc agaaacgcgg gcagtccagc tcgacccgct gcgtggcgta gggtgttgcg
     6241 ggcgcggcgc cctcgggcat gaccgcgacc gtgacgtgcg tcgggaccgg cgtcagcgcg
     6301 atgacggaga ccgaccagcg ggtcccgccc gggtcgctcg tcccgcgccg gcatccgttc
     6361 cagccctgac acgccgtctt cggcgtcgag acctcttcgc ctgcgcgcag cagcgccgtg
     6421 cgcaccgcgc tgcccgccgg cccctgcgcc tggccgttga aggtgacccg gaagctcgtg
     6481 ccggcgcgca ggcgctcgca gagcacgcgc gtgatctcga cactcgcgtc cggacgcgaa
     6541 gtctgttcgg cgcgcggtgc ctcggtgcgc gcggcgggtg gcgcgggggc gcccttcgct
     6601 gcacgcgcag cgtccgggcg cacgccccaa ccttccagga cgaagccggc gaggtccgcg
     6661 ggcaccgcac gggcgaactt gccgtcgagc agcgtgacca cccccaccac gttgtctccc
     6721 atcagcaccg gtccgccgga gttgccctcg tcgatgctgc cgtcgagcat caggtcgcgg
     6781 ccctcgcgcg aaaccacggt ggcgtgcacc accgcccagg ccccgccgcc ccgtgggaag
     6841 ccgatggtcg tcaccgactc gcccccgcgc agatcggcgc cgcggcgcag cggcagcgcg
     6901 ccgatgccgg ccggcagccg gtccttgccg cgtaccacca gcagcgccag tccacgcggg
     6961 tcgccgccct cgatgcgcgc cgtctccgct tcgacggttg cgttgcgacg cgtgaagaac
     7021 tcgataagcg ggcggttgtc gccctcgacc acatgcgagg cggtgacgat gtacgcggcg
     7081 tcggcgtcca ggtgcacgat gaaaccggtg ccggtcctgc gcgccccgtc cagcctcgac
     7141 gtcaccttga ccacgccagc tttctgccga tccaggtcct gcggccaggc ggcacccgcg
     7201 atcagcagca gcacccaaca gcagatgcag cgtgcagccg agcgcttcat gcggcggcgt
     7261 gctcttcgta caggcggtag cccccggcca gcatcgaagc caggcagact gcggcgagca
     7321 gcgcgacgcc gacgcggctg cggtgccaca ggctctgcag cgcggtgatg cacaggctcc
     7381 gctcggcgcc accggacgcg agggtggcct gggtcgccac accgtccatg gtcagcagca
     7441 tgctcgactg ctcggccgac aactccaagc gcgttatagt ggtccggctc atgtcgccga
     7501 gcgtgacgaa gtcccgcgcg ccgaccggta ccggtgcgat gcgcggctgc tgtggatagc
     7561 ggatcgcgcc gttcgcgatg acggcgctct cgacggttcc acccccgccc tgcttgagca
     7621 actccacgcc gttcacgccg gcgcggtcca gcaaggcgag cgcggcgctc gcgctgagcg
     7681 tggccgtgat cgcgaaccag cggcgcgtgc cctgcacctg aacgaaggga cgatcctccg
     7741 gcagcgagaa cgcgagcgca accgggccgt ctgccggcgc cgagggcgac gattcgccca
     7801 gcgtgacgtt ggtggcggaa agcgccagct ccgcggcggg caggatgttg gtgtccagcg
     7861 gctgtccctc cacccgcagc gtgacgcgat tcgaggcatc ggcgctcgtc gccaggatca
     7921 cccggctgcg cggcgcgatc gccagcggct gcatgcgtcc ggcgccggct gtgctgttgc
     7981 cgcgccccac accttccacg accgctaccg ccgccgcgtc tgccgggctg ttcaggatca
     8041 cggtggcgga cctgggcggc atgcgggaag atccgggcgc gatccagcgt acccggtcgg
     8101 gcgtgaaggt cagccgatcg aagttctcga cgctcagcgt gcggaaaccg agcgggcgca
     8161 ccggcatatc gaccccccgt tccccggcga gcgtgaacgc gagccggtcg acgacgagat
     8221 cgagctgcaa ccgcgtcggc acgcgccaca tcgtcgcggc gacggccgca gccagaatcg
     8281 ccacccccgg cgccaggagc atcaaggcca ggcccgggcg gcgtggcccg cgctgcgtag
     8341 cgctgtcccg cgcccggaga ggcccgggcg gtcgatgggc gcgggcggcg ggctgcatcc
     8401 cggcgtgcgc gccggccgcg cccgggtcgt gttgcgcagc tcccgcgcgg ccgccgatgg
     8461 cggtgatgtg agcaagcagc gcggagaact ccgcgtgctg cgcatcgccc gaccagtccg
     8521 acaggttggc cgcctgcagc agcctgaact ccagcggaag cttcagctcg ggctccatca
     8581 gcaccgggac cagcacctcg cgtgccgcgg cctccgacgc ttcggcgcga acccacctcg
     8641 actgcaccga tgcagccgtc cacagcacca gcacgcattc ggcctgcgcc aggttctctt
     8701 cgatgacctg gtcgaaggag ctgccgagcg ggatcttgcg gtcccaccac accgcaaacc
     8761 cgctctgcgc cagggcctgc gccaggcgct cggcgcccgg gcgatcctcg cttgcgtagc
     8821 tgatgaagac gcgaaccatc cagtcggtca gcggacctga ggccgatgat ggaatctcac
     8881 gacctggctg atcggatcgt tggtgctcag caggtagcct gacaggacta ggcggtacgc
     8941 gccttccggc gggccgccct cggcgagatc ccacctgtac gagaacggcc ggccggcggt
     9001 acgccgcacc gacggggcag accagatcgg ctccgtgccc gtgtccttga agatcgcggc
     9061 gcgcagcatc gcttcctctc gcagcctgaa atggaacacg taaccgcgga ctgcggcggg
     9121 gaactgcgat tggtagaaca gcgccggcgc aacctcctcg atcgccgacg gcgtggcccg
     9181 gtccagccgc accaccacgc cgaggtccga gatgcgcaag tcgccgagct gccgcacgac
     9241 ctcggccgtc gaccagctga acacgttcgc gaagccggcg cgccacggcg attcgggctg
     9301 cacccggtcg agccaataga agtagcggta gtcgagctcc cggaccacca ggtggacggc
     9361 gcgtggacgc gtcaggaaga agcgcacgtg gaagcgctcg ccgatcttct ccgcatcgtc
     9421 ggcgtaggcc gcctgtgcgg cgagcagctc gatgtcgaag ccggacacgg ggcgcgcacg
     9481 cacgccctcg tagtggttcg cgcgcttctg gtattgcagc tgcgtctgcg cctgcacgca
     9541 ggcgcactcg caggccagcg cgacgatgac gatcggaaac agcgcgaacc ggcgctttgc
     9601 ctcgaaccta tccgcccagc atcgccagga tctgccgcca gttgagcacc acataaccca
     9661 ccgccatcac catgtaagcc gccgtgcaca gcagcatcaa cgcctgcgtg accggatgcg
     9721 gccggatcca cttcgggaac accctgggca gcatcacgta gttcagatac acgaacgcc
//
LOCUS       DASPGE010000412         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_346818, whole genome shotgun sequence.
ACCESSION   DASPGE010000412 DASPGE010000000
VERSION     DASPGE010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346818"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VD791_13560"
     CDS             complement(<1..217)
                     /locus_tag="VD791_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV09055.1"
                     /translation="MTGLRAIALPAAPEGYTSGSPAQRLGALVYSSGVDHARPMSSAA
                     PSVHPRASALEVLLVFLKLGLTSFGGPV"
     gene            263..1231
                     /locus_tag="VD791_13565"
     CDS             263..1231
                     /locus_tag="VD791_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXV09056.1"
                     /translation="MPLAARICAVALAAVCAPGAAAAADFPSKPLRIIVPFPPGGFND
                     QLARILGQKFTARWRQPVVVDNRAGGSTLVGTDLAAKAPPDGHTLLVVSFAFAVNPSL
                     HARLPYDTLRDFTPVVLAAGAPNFLVVNRNMPVKSVRELIRAAKARPGAIDYASGGNG
                     TSPHLCMELLKSMAGIDLVHVAYKGSAPAVTDLIGGQVQVAFDNAPNVLPHVKAGKLH
                     ALAVSTAARSPLAPELPTIAESGVPGFDVEVWFGIVAPAGTPRDVVALINAEINRALA
                     LPEVNRRFAEQGVRTIGGTPEQFGAYLQGQIARWAKVVKDTGVRIE"
     gene            1287..2054
                     /locus_tag="VD791_13570"
     CDS             1287..2054
                     /locus_tag="VD791_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXV09057.1"
                     /translation="MDEVTTERRGDIALVTLNRPQSLNAVNRALRRALIAALTQANAD
                     AGVRAVVIAGAGDRAFCSGQDLSETAEYTVNDIDQWLSEQHAMYQAVRDLDKPCVAAW
                     NGVAAGAGYQLGLCADLRVGYPQMKLGQPEIKAGLASIVGSYLMTLHLTLSQNQQLSI
                     TGELIDGQRAYELGLINYLVPRDQVLSKAIAVAQELTRLGATALRLTKRRFRELTQPG
                     FDAALEAARQIQKEAYASGEPQAAMKRFFETRARKTS"
     gene            complement(2074..>2649)
                     /locus_tag="VD791_13575"
     CDS             complement(2074..>2649)
                     /locus_tag="VD791_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXV09058.1"
                     /translation="GANYAEAYAASGERARQTGALEVHAYDQAEVLAGQGTLARELER
                     QTEGLDTVLVGVGGGGLIGGVAAWFAGRVKLVAIEPERCPTLHAAVQAGAPVDVEVGG
                     IAADSLGARRVGGLMFDIAQRFVDAVVLVSDEEIRQSQRALWDSLRVLAEPGGAAALA
                     GLRSGRYQPAAGERLAVVVCGGNTDPARFSA"
BASE COUNT          425 a          936 c          923 g          365 t
ORIGIN      
        1 ccaccgggcc gccaaaggaa gtgaggccca gtttcaggaa caccagcagc acctcgagcg
       61 ccgatgcgcg cgggtggacg gagggggcag cggaagacat gggccgagcg tggtcgacgc
      121 cggaactgta caccagcgcc cccagccgct gcgcaggcga gccgctggtg tatccttcgg
      181 gcgcggctgg tagcgcgatg gcccgaagcc cggtcatcgc cacacgagca aactgggccg
      241 cgcgaggagg agatcgacct gcatgccgct cgctgcacgg atctgcgccg tcgcgctcgc
      301 cgccgtgtgc gcgccgggag ccgcggcggc cgccgacttt ccgagcaagc cgctgcgcat
      361 catcgtgccg ttcccaccgg gcggcttcaa cgatcagctg gcgcgcatcc tgggccagaa
      421 gttcaccgcg cgctggcgcc aaccggtggt ggtcgacaac cgcgcgggcg gcagcactct
      481 ggtcggcacc gatctcgccg cgaaggcgcc gcccgacggc cacacgctgc tggtggtgtc
      541 gttcgccttc gccgtcaatc ccagcttgca cgcacgcctg ccgtacgaca ccctgagaga
      601 tttcacgccg gtcgtgctgg ccgcaggcgc gcccaacttc ctggtcgtga atcgcaatat
      661 gccggtcaag tcggtgcgcg agttgatccg ggcagccaag gcccgccctg gcgcgatcga
      721 ctacgcctcg gggggcaacg gcacctcccc gcacctgtgc atggagctgc tcaagagcat
      781 ggccggcatc gacctggtgc acgtcgccta caagggcagc gcaccggcgg tcaccgatct
      841 gatcggcggc caggtgcagg tggcgttcga caacgcgccg aacgtgctgc cccacgtgaa
      901 ggcaggcaag ctgcacgcgc tcgcggtatc caccgccgcg cgctcgccgc tggctcccga
      961 gctgccgacc atcgccgaga gcggcgtgcc gggcttcgac gtcgaagtgt ggttcggcat
     1021 cgtggcgccg gccggcacgc cgcgggatgt ggtggcgctg atcaacgcgg agatcaaccg
     1081 cgcgctcgcg ctgcccgagg tgaatcgccg cttcgccgag cagggcgtgc gcaccatcgg
     1141 cggcacaccg gagcagttcg gggcgtatct gcagggtcag attgcgcgct gggccaaagt
     1201 ggtgaaggac accggcgtgc ggatcgagta aagggttcgc ttgcgcacca agcaggtgag
     1261 gagcgtttct cgggagatca tcgaccatgg acgaagtcac gacggagcgc cggggcgaca
     1321 tcgctctggt gacgctgaat cgcccgcaat cgcttaacgc cgtgaaccgc gcgctgcgcc
     1381 gggcgctgat cgccgcgctg acgcaggcca atgccgacgc gggcgtgcgg gcggtggtga
     1441 tcgccggcgc cggcgaccgc gccttctgct cggggcagga cttgagcgag accgccgaat
     1501 atacggtgaa tgacatcgat cagtggctga gcgagcagca tgcgatgtat caggcggtgc
     1561 gcgatctgga caaaccgtgc gtggcggcgt ggaacggcgt ggccgccggc gccggctatc
     1621 aactcgggct atgcgccgac ctgcgcgtgg gctacccgca gatgaagctg gggcagcccg
     1681 agatcaaagc cgggctggcg agcatcgtcg gctcgtacct gatgacgctg cacctcaccc
     1741 tgtcgcagaa ccagcagctg tcgatcaccg gcgagctgat cgacggccag cgcgcctacg
     1801 agctgggatt gatcaattac ctggtgccgc gcgatcaggt cctgagcaag gcgatcgcgg
     1861 tcgcgcagga attgacgcgg ctcggcgcca cggcgctgcg cctcaccaaa cgccgcttcc
     1921 gcgagctgac ccagcccggc ttcgacgccg cgctggaggc ggccaggcag atccagaagg
     1981 aagcctacgc cagcggcgag ccgcaggcgg cgatgaagcg cttcttcgag acgcgtgcga
     2041 ggaaaacctc ttgaacccct ttagccgccg gcatcacgcc gagaagcggg cggggtcggt
     2101 gttgccgccg cacacgacca cggcgaggcg ctcgcccgcg gccggttgat atcggccgga
     2161 gcgcaggccg gcgagcgccg ccgcgccgcc gggctcggcc agcacgcgca agctgtccca
     2221 cagcgcgcgc tggctctggc ggatctcttc gtcgctcacc agcaccaccg cgtccacgaa
     2281 ccgttgcgcg atatcgaaca tcaggccgcc gacccggcgc gcaccgagcg agtccgcggc
     2341 gatgccgccc acctccacgt ccaccggcgc gcccgcttgc accgcggcgt gcagcgtcgg
     2401 gcaacgttca ggctcgatgg cgaccagttt gacgcggccg gcgaaccagg ccgccacgcc
     2461 accgatcaag ccgccgccgc ccacgccgac cagcaccgtg tccaggccct cggtctgacg
     2521 ctccagctcg cgcgccagcg taccctgccc ggccaggact tcggcctggt cgtaggcgtg
     2581 cacctccagc gcgcccgtct ggcgagcgcg ctcgccgctg gccgcgtagg cttcggcgta
     2641 gttcgcgcc
//
LOCUS       DASPGE010000413        13428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_228303, whole genome shotgun sequence.
ACCESSION   DASPGE010000413 DASPGE010000000
VERSION     DASPGE010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13428
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228303"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /gene="ruvB"
                     /locus_tag="VD791_13580"
     CDS             complement(<1..734)
                     /gene="ruvB"
                     /locus_tag="VD791_13580"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HXV09059.1"
                     /translation="MIETDRLIAPERTSPQEEALERTLRPKHLNEYVGQEKIRGQLSI
                     FIEAARRRREALDHVLLFGPPGLGKTTLAHIIAREMGVNLRQTSGPVLERPGDLAALL
                     TNLEPHDVLFIDEIHRLSPVVEEILYPALEDFQIDIMIGEGPAARSLKLDVPPFTLVG
                     ATTRAGMLTNPLRDRFGIVSRLEFYTSDELASIVQRSARLLNVGVEGDGALEIASRSR
                     GTPRIANRLLRRVRDYAEVKDRGVVTR"
     gene            complement(742..1518)
                     /locus_tag="VD791_13585"
     CDS             complement(742..1518)
                     /locus_tag="VD791_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV09060.1"
                     /translation="MNHRHTIPLAGLLAGCLALLCAVPAAASKTEKSERLFNLHDLRT
                     TVAIGDYYLKQESVLAARAYLRQLGREQDLGSGWNPANAHWQQAEQGIVNALMRRVGR
                     EFSSMEWLSEEWARLNSAELSEEDMDTLLAHFGTDVGRKQVMIVDHSVAFHVMAALSL
                     GGKIQENVPGTEAERKRMQDLYNAEDAAMRFDHNESPEATRFAFSPVGKKYFVNAVLK
                     VSGLVSRRLYQTAAEMPARVSDAAAEAQSSVDAYRKARSG"
     gene            complement(1571..2152)
                     /gene="ruvA"
                     /locus_tag="VD791_13590"
     CDS             complement(1571..2152)
                     /gene="ruvA"
                     /locus_tag="VD791_13590"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HXV09061.1"
                     /translation="MIGRITGIILEKHPPQVLVDVQGVAYELDVPMSTFYNLPANGVR
                     ITLHTHLIVRADAHLLFGFASDAERQAFRQLLKISGIGARTALAVLSGMSVDELQQAV
                     TARDAARLIKVPGIGRKTAERLLLELRDKLQTGAGGAVAGAAQSARGDALNALLSLGY
                     GEKEASAALTQLAPDLNTAESIRQALRLLSKVL"
     gene            complement(2155..2673)
                     /gene="ruvC"
                     /locus_tag="VD791_13595"
     CDS             complement(2155..2673)
                     /gene="ruvC"
                     /locus_tag="VD791_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HXV09062.1"
                     /translation="MRILGIDPGLRVTGFGLLDRAGQTLRYVGSGVVRTPDLALPGRL
                     KVILDSLSEVIAAHGPHQVAVEKVFVNVNPESTLLLGQARGAAICAAVSHGLPVAEYT
                     ALQVKQAVVGNGHAAKEQVQEMVRRLLTLPGDPGADAADALACAICHAHGGLGLGALA
                     TAGYRMRRGRLV"
     gene            complement(2701..3429)
                     /locus_tag="VD791_13600"
     CDS             complement(2701..3429)
                     /locus_tag="VD791_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXV09063.1"
                     /translation="MAGHSKWANIKHKKAAQDAKRGKVFTRLIKEITVAAKLGGADPS
                     MNPRLRLAIDKAYEANMPKDNVERAIKRGSGELEGVDYEEIRYEGYGTGGAAVIVDCL
                     TDNRTRTVADVRYAFGKYGGNLGTEGSVSFLFKHCGLLVFAPGTSEDAVMEAAIEAGA
                     EDAVVNDDSSIEVIAAPQDFPAVRQALERAGLKPELAELTMKPTTENVLSGEDAARMQ
                     RLLDALESLDDVQAVYTTAVIEDQ"
     gene            3563..5071
                     /locus_tag="VD791_13605"
     CDS             3563..5071
                     /locus_tag="VD791_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HXV09064.1"
                     /translation="MPPPLAIAIAAHQICLLPGLANRHGLITGATGTGKTVTLQCMAE
                     GFSRIGVPVFVADVKGDLSGMSRPGEMTPKLRERLQSHGLPEPQFGACPVTFWDVYGQ
                     LGHPVRTTVSEMGPLLLARMLNLNETQSGVLTLVFKIADDNGLLLLDLKDLRAMCQFV
                     GDHARQFTTDYGNVSAASIGAIQRGLLALGEQGGERFFGEPALDIPDLMHTDGHGRGR
                     INVLAAERLMQQPKIYATFLLWLLSELFETLPEAGDPDKPRLVFFFDEAHLLFDDAPQ
                     ALLDKIEQVVRLIRSKGVGVYFVTQNPLDVPERVLGQLGNRVQHALRAFTPRDQKAVK
                     AAAETLRTNPQLDAEKAITELAVGEALVSFLDEKGRPAVVERAWILAPGGRIGPVTPE
                     ERKAAIAGSSLYGRYENAQDRESAYEKLKTHAAQRLPEMSSMPAPSTAGGAMDALRDI
                     LIGSTGPRGGRREGMLEAAAKSAARSVGSQLGREIMRGVLGGLLGGATRRER"
     gene            5322..7475
                     /locus_tag="VD791_13610"
     CDS             5322..7475
                     /locus_tag="VD791_13610"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HXV09065.1"
                     /translation="MSALTTPALGGIDVLRLPCMPLVLRTALERAGDGRQLGPLAETL
                     AQDPGLAVRFLATTSSATATPQGTGASLGRHVELLGSGLAQSILMRAATDRLRRTTAG
                     LASPDALAFWAHSLHCALLAQTIAETAAYAQPEEAYLAGLLHDLGIVALAIDIPHTYG
                     NAFAGASFEAELLERELDQLRTSHAEVSAALVARLGLSGHLADAVLLQHAPYDELTGT
                     HKLVRILWVAEAYTDRSVATPDVAGFARLIGMPQEALVSANDQAAARYLEALRLLGTP
                     AIPQSRLWRLPAPAAAAAENETTWQRSADGALLDTVMQSASLQQVPFLLLGAEDTGAV
                     LSRLRSITAALFGLQTFAAFLHDPAARSLNGWLVTVDRLAPTELEIPVTGGTSLVARA
                     AAERQALRTQGGEGGERLRGVDLEIGRLLQADALSIVPLIAGGNLLGVLVFGVGREQA
                     PRLAGDSLMLQRLSEMATLALTNRRQSAEQQRLKEADLRERLRLSARRLVHEARNPLS
                     VMKTHLELLGERVQSGQPIDKDLRVLREEIDRVGEIVARIGTTDLSVEKGHAPVDING
                     LIRELMIVYREALFATRGVNVELELDPRLPGIVTDGNALKQVLLNFWKNAAEAMPAGG
                     QVRVRTADRVNYEGQLMLEITVSDTGSGMPPEVLENVFSQRLTLTKGSERGYGLSNAF
                     TLVKQLGGHVLCRSEPERGTLFTVLLPRTLHAPGT"
     gene            7486..9669
                     /locus_tag="VD791_13615"
     CDS             7486..9669
                     /locus_tag="VD791_13615"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXV09066.1"
                     /translation="MPDLGLPPADAPGLYLPGSEAIAEGARILLVDDEPRIREAVEAL
                     IAQPGRQILHCGSGGEALRILGGGQVDLALLDIRLPDMTGLEILERVRREGSSVRVII
                     VSADDQVDSAIAALRFGAYEYVRKPFEPAALRRTVDNALSQLRLERDNERMRGRLEQS
                     ERLHRYLIDQSPDLIYTLDRDGRFTFVNERFHRLLGFERDELIGTHYSSIVYREDVKR
                     AEYAFNERRTGQRASSNVELRVTRKGGDGTPCTIVLSAVGVYARREAGSDARSGFVGT
                     YGVGRDISDRKRAEEIVSYHAFYDVLTGLPNRTLFRDRLRQAIAQARRRRSRVAVMFI
                     DLDRFKLVNDTYGHVRGDLFLQQVAGRLRRQLRSSDTLSRLGGDEFTVLVPDLRTPED
                     AALKAQQFLEALRRPFVVEGEELAATASIGLAVFPDHGASEDELVRNADLAMYQVKRR
                     GKDGVGFFAKEMSSLYSEKINLEGELRKALQRADFQVLYQPIHDVRKGSVECVEALLR
                     WQHPTLGTLEPARFIYIAEETGMISLLSDRVLDSACAQLKQWRAEGFHKLRVAVNVSA
                     RDFERSDFTERVVQALTRHKLQPSALELEITENVLIEDVDAAVDKIRQLRKTGVRISI
                     DDFGTRYSSLGYLQRLPVNTIKIDQVFTRELDEAQPQSPIVLAIVGIAKGFGLHLVAE
                     GVERAVHRDMLRNLGCQSMQGFLFSPALTAEETTSYLRRAHDGTL"
     gene            complement(9685..10773)
                     /gene="ychF"
                     /locus_tag="VD791_13620"
     CDS             complement(9685..10773)
                     /gene="ychF"
                     /locus_tag="VD791_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HXV09067.1"
                     /translation="MKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGIVAV
                     PDARLERIAAIAKPQKAIPAVVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIAHVV
                     RCFEDPNVVHVAGKVDPVSDIETINTELALADLATVEKQLAKYSKVAQAGGDKEAQRL
                     VAVLEKVQPALDQARPARSVPLYPEETELIRPLFLLTMKPTMYVANVAEKDFERNPLL
                     ERVRQFARGEGAPVVAICAALEAQIVDLSDEDKRVFLADLGLEEPGLNRLIRAAYELL
                     GLQTFFTAGPKEVRAWTVHRGDTAPMAAGVIHTDFEKGFIRAEVIAFDDYVALKGEQG
                     AKEAGKMRLEGKDYVVRDGDVVYFRFNV"
     gene            complement(10899..11474)
                     /gene="pth"
                     /locus_tag="VD791_13625"
     CDS             complement(10899..11474)
                     /gene="pth"
                     /locus_tag="VD791_13625"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HXV09068.1"
                     /translation="MRLVVGLGNPGREYETTRHNAGFWWLENLAEIERVSLKTESRFH
                     GRVGRIAAHGKDCWLLLPQTYMNDSGRSVAALAGFYKIAPEEILVVHDELDLPPGAVR
                     LKKGGGVAGHNGLKDVARHLGPEFWRLRLGIGHPGERAEVVNYVLRPPREEETRLISD
                     AVERSLEVWPLIFAGEMEKAMHRLHTRDSDS"
     gene            complement(11549..12277)
                     /locus_tag="VD791_13630"
     CDS             complement(11549..12277)
                     /locus_tag="VD791_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HXV09069.1"
                     /translation="MPIEVNATPRSTQGKGASRRLRHAQRVPGILYGGGQPAASIEVD
                     HKELAQHLKHETFHASILTLNMEGSKLPVLLRDYQMHPWRQQVLHVDFQRVAKDRKIH
                     MKIPLHFVNAEVAPGVKTGGGSVNHVLTEIDVTCLPDDLPEYIDVDLSNLQMGHSIRL
                     SELKLPKGVESSQLRGGDDAVVVTIPVPKAEPVVEEEVAPVTTAVAAPAAAVPGAPPE
                     KAEGEKEKEEKEKKEERKDRERKK"
     gene            complement(12418..13368)
                     /locus_tag="VD791_13635"
     CDS             complement(12418..13368)
                     /locus_tag="VD791_13635"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HXV09070.1"
                     /translation="MAYDSLMVFTGNANPALAQDVVRHLNIHLGRATVSRFSDGEVMV
                     ELLENVRGKDVFVLQSTCTPTNDNLMEVMLMVDALKRSSAERITAAIPYFGYARQDRR
                     PRSARVAISAKVVANMLQAVGVDRLLVMDLHSDQIQGFFDIPVDNIYAAPILLGDVWK
                     HGHQNLVVVSPDVGGVVRARALAKRLDSDLAIIDKRRPRPNVATVMNIIGEVKGRTCI
                     IMDDMVDTANTLCEGALALKEKGAERVLAYCTHPVLSGNAVQRIENSEIDELVVTDTI
                     PLNGDARASKRIRQLAVGELLAETMLRISNEESVSSLFMD"
BASE COUNT         2062 a         4663 c         4504 g         2199 t
ORIGIN      
        1 cgcgtcacca ccccgcggtc cttgacctcg gcatagtcgc gcacgcggcg cagcaagcgg
       61 ttggcgatgc gcggcgtgcc gcgcgaacgc gaggcgatct ccagcgcgcc gtcgccttcg
      121 accccgacgt tgagcagccg cgccgaacgc tgcacgatcg aggccagctc gtcgctggtg
      181 taaaactcca gacgcgagac gataccgaag cggtcccgca gcggattggt cagcatgccg
      241 gcgcgcgtgg tggcgccgac cagcgtgaac ggcggcacgt ccagtttcag cgaccgcgcc
      301 gccggaccct cgccgatcat gatgtcgatc tggaagtcct ccagcgccgg atagaggatc
      361 tcctcgacca ccggcgacag gcggtggatc tcgtcgatga acaacacgtc gtgcggttcg
      421 agattggtga gcagcgccgc caggtcgccc ggtcgttcca gcaccgggcc ggaagtctgg
      481 cgcaggttca cacccatctc gcgggcgatg atgtgcgcca gcgtggtctt gcccaggccc
      541 ggcggcccga acaacagcac gtggtcgagc gcctcgcggc gccggcgcgc ggcctcgatg
      601 aagatcgaca gctggccgcg aatcttctcc tgcccgacgt actcgttcag gtgcttgggc
      661 cgcagcgtgc gctcgagcgc ctcttcctgg ggcgaagtgc gctcgggtgc gatcaaccgg
      721 tcggtctcga tcacgcgctg ctcatcccga tcgcgccttg cggtaagcgt ccaccgagct
      781 ctgcgcctcg gccgcggcgt cgctcacgcg cgcgggcatt tccgccgcgg tctggtacag
      841 ccggcggctc accagcccgc tcaccttgag caccgcgttc acgaaatact tcttgcccac
      901 cggcgagaac gcgaaccggg tggcctccgg gctctcgttg tggtcgaagc gcatcgccgc
      961 gtcttcggcg ttgtacaggt cctgcatgcg cttgcgctcg gcctccgtgc ccggcacgtt
     1021 ctcctggatc ttgccgccca ggctcaatgc cgccatgacg tggaaggcga cgctgtggtc
     1081 cacgatcatc acctgcttgc gacccacgtc ggtgccgaaa tgggcgagca gcgtgtccat
     1141 gtcttcctcg ctcaactccg cgctgttgag ccgggcccat tcctccgaca gccactccat
     1201 gctggaaaac tcgcggccca cgcgccgcat caacgcgttc acgatgccct gctccgcctg
     1261 ctgccagtgg gcgttcgccg gattccaccc gctccccagg tcctgctcgc gtcccagctg
     1321 ccgcagatat gcgcgcgccg ccagcacgct ctcctgcttg agatagtagt cgccgatcgc
     1381 caccgtggtg cgcagatcgt gcagattgaa cagccgctcg ctcttttcgg tcttggacgc
     1441 ggccgccgga acggcacaca ggagcgccag gcagccggcg agcaggccgg cgagagggat
     1501 ggtgtgccga tggttcattc gtcgtgccgt ccgggtgggc gcgaaaaaac cgctgtcgaa
     1561 gtggcgccga tcatagcacc ttggacagca ggcgcagcgc ctgacggatc gactcggcgg
     1621 tgttcagatc gggcgcgagc tgcgtgagcg cggcgctcgc ctccttctcg ccgtaaccca
     1681 gcgagagcag cgcgttgagc gcgtcgccgc gcgccgactg cgccgcgcct gccacggccc
     1741 cccccgcccc ggtctgcagc ttgtcgcgca gctcgagcag cagccgctcg gcggtcttgc
     1801 gcccgatgcc cggaactttg atgagccgcg cggcgtcccg cgcggtcacc gcctgctgca
     1861 gctcgtccac ggacatgccg gacagcaccg ccagcgcggt gcgcgcgccg atcccgctga
     1921 tcttgagcaa ttggcggaac gcctggcgct cggcatcgct cgcgaagccg aacaacaggt
     1981 gcgcgtccgc gcgcacgatc aggtgggtgt ggagcgtgat gcgaacgcca ttggccggca
     2041 ggttgtagaa agtgctcatc ggtacatcga gctcataggc gaccccctgc acgtccacca
     2101 ggacctgtgg cgggtgcttc tccaggatga tgccggtgat gcgcccgatc atgctcagac
     2161 caagcgcccg cgccgcatcc ggtatccggc ggtagccagc gcgcccaggc cgagcccacc
     2221 gtgtgcatgg cagatggcgc aggcgagcgc gtcggcggcg tcggcgccgg gatcgccggg
     2281 caacgtgagc agccggcgca ccatttcctg cacctgctcc ttggcggcat ggccgttgcc
     2341 gaccaccgcc tgcttgacct gcaacgcggt gtactcggcc accggcaatc cgtgcgagac
     2401 ggcggcgcag atcgccgcac cgcgcgcctg cccgagcagc agcgtcgatt ccgggttgac
     2461 gttgacgaag actttctcca ccgcgacctg gtgcggcccg tgcgcggcga tcacctcact
     2521 cagactgtcc aaaatcacct tgaggcgccc cgggagcgcc aggtccggcg tgcgcaccac
     2581 gccgctgccg acgtagcgca gcgtctgacc ggcgcgatcg agcagaccga acccggtgac
     2641 ccgcaggccg gggtcgatgc cgaggatgcg cactgcgtcg gtcgggccgc ccacgccggc
     2701 ctactggtcc tcgatgaccg cggtggtgta taccgcctgg acgtcgtcca gggactcgag
     2761 cgcgtcgagc agccgttgca tgcgcgccgc gtcctcgccg ctcagcacat tctcggtggt
     2821 cggtttcatg gtcagctcgg ccagctcggg cttgaggccg gcgcgttcca gcgcttgccg
     2881 caccgccgga aaatcctgcg gtgcggcgat cacctcgatg ctgctgtcgt cgttgaccac
     2941 cgcgtcctcg gcgccggcct cgatggcggc ctccatgacc gcatcctcgc tggtgccggg
     3001 agcgaacacc agcagcccgc aatgcttgaa caggaacgac accgagccct cggtgccgag
     3061 gttgccgccg tacttgccga aggcatagcg cacgtcggcc acggtgcggg tgcggttgtc
     3121 ggtcaggcaa tccacgatca ccgccgcgcc gccggtaccg tagccctcgt agcggatctc
     3181 ttcgtagtcc accccttcca gctcgccgct gccgcgcttg atcgcgcgct cgacgttgtc
     3241 cttcggcatg ttcgcctcgt aggccttgtc gatggccagg cgcaggcgcg ggttcatgct
     3301 cgggtcggcg ccgccgagct tggcggccac cgtgatttcc ttgatcagcc gggtgaagac
     3361 cttaccgcgc ttggcgtcct gcgcggcttt cttgtgcttg atgttggccc acttgctgtg
     3421 acctgccatg gatgcggcgc ctgccggcgc ggacggtgat cggaacgcgg ggacgtcggg
     3481 aacgtgcgcg cggatcatgg accgcttgcg cgtgctgaat ataatcggca aagtctagca
     3541 cagccgcacc cggaacccgc cgatgccccc gccccttgcc atcgccatag ccgcgcacca
     3601 gatctgcctg ctgccgggcc tggccaaccg ccacggcctg atcaccggcg ccaccggcac
     3661 cggcaagacc gtcacactgc agtgcatggc cgagggcttc agccgcatcg gcgtgccggt
     3721 attcgtggcc gacgtcaagg gcgatctatc cggcatgagc cggcccggcg agatgacgcc
     3781 gaagctgcgc gagcgcctgc agtcccacgg cctgcccgag ccgcagttcg gcgcctgtcc
     3841 ggtgaccttc tgggacgtat acggccagct cggccatccg gtgcgcacca cggtctcgga
     3901 aatggggccg ctgctgctgg cgcgcatgct gaatctgaac gaaacccaga gcggcgtgct
     3961 gacgctggtg ttcaagatcg ccgatgacaa cggcctgctg ctgctggatc tgaaggacct
     4021 gcgcgccatg tgccagttcg tcggcgacca cgccaggcag ttcaccacgg actacggcaa
     4081 cgtctctgcg gccagcatcg gggcgatcca gcgcggcctg ctggcgctcg gcgagcaggg
     4141 cggcgagcgc ttcttcggcg agcccgcgct cgacatcccc gatctgatgc ataccgatgg
     4201 ccatggccgg gggcggatca atgtgctggc ggccgagcgg ctgatgcagc aaccgaagat
     4261 ctacgccacg ttcctgctgt ggctgctgtc cgagctgttc gaaacgctgc ccgaggcggg
     4321 cgacccggac aagcccaggc tggtgttctt cttcgacgag gcgcacctgc tgttcgacga
     4381 cgcgccgcag gccctgctcg acaagatcga gcaggtggtg cggctgatcc gatccaaggg
     4441 cgtgggcgtg tacttcgtca cgcagaatcc cctcgacgtg cccgagcggg tgctcggcca
     4501 gctcggcaac cgcgtgcagc acgcgctgcg cgctttcacc ccgcgcgatc agaaagcggt
     4561 gaaggcggcg gccgagacgc tgcgcaccaa cccgcagctc gacgccgaga aggcgatcac
     4621 cgagctcgcg gtgggcgaag cgctggtctc gttcctcgac gagaaaggcc gcccggccgt
     4681 ggtcgagcgc gcctggatcc tggcgcccgg cggacggatc ggcccggtca caccggagga
     4741 gcgcaaggcg gcgatcgcgg gctcgagcct ctacggccgc tacgagaacg cgcaggaccg
     4801 cgagtcggcg tatgaaaagc tcaagaccca cgccgcccaa cgcctgcccg agatgtcgtc
     4861 gatgcccgcg ccgtccacgg cgggtggcgc gatggatgcc ctgcgcgaca ttctgatcgg
     4921 gtcgaccggg ccgcggggcg gccggcgcga gggcatgctg gaagccgccg ccaagagcgc
     4981 ggcgcgctcg gtcggatctc agctcggacg cgagatcatg cgcggcgtac tgggcgggct
     5041 cctgggcggg gcgacccgga gggagcgctg agtgcgcgaa atgctcacgc atgtgcgtgc
     5101 cgaacggcgc cttacgggtg ccgcccagga cgggtcggcc gccctccctt cggccgccaa
     5161 ccgggcgtct ctttctggag acatgcgctg caaccccttg aaccaagtgt tacattggtt
     5221 ttgcagggat ggacgctata ggtgccaagt ggcaccatcc ggcagacacg gagaccgccc
     5281 cgtcgggcgc ccgataacta taacgtcatt agaaagcagg catgagcgcc ctcaccacgc
     5341 ccgctctggg gggcatcgac gtgttgcggc tgccttgcat gccgctcgtc ctcagaacag
     5401 cgctggagcg tgccggagat ggccgccagc tcggcccgct ggcagagacg ctggcgcagg
     5461 atcccggtct cgccgtcagg ttcctggcca cgacgtcgtc cgcgacggcg accccgcagg
     5521 gcacgggcgc ctcgctcggg cgccacgtgg agctgctcgg cagcggcctc gcgcaatcga
     5581 tcctgatgcg ggcggcaacc gaccgcctgc gccgcacgac ggcggggctt gcttcgcccg
     5641 acgcgctcgc gttctgggcg cactcgctgc attgcgcgct gctcgcgcaa accatcgccg
     5701 agacggccgc ctacgcgcag cccgaggaag cctacctggc ggggctgctg cacgacctgg
     5761 gcatcgtcgc gctcgccatc gacatcccgc atacctacgg caacgcgttc gcgggcgcct
     5821 ccttcgaagc ggagttgctc gagcgcgagc tcgaccagct gcgcaccagc catgccgagg
     5881 tgtccgccgc gctggttgca cgcctcggcc tgtccggcca cctggccgac gcggtgctgc
     5941 tgcagcacgc cccctacgac gagctgaccg gtacgcacaa gctggtgcgc attctgtggg
     6001 tggccgaagc gtatacggat cgctcggtcg ccacgccgga tgtggccggc tttgcgcgcc
     6061 tgatcggcat gccgcaggag gcgctggtca gcgccaacga ccaggcggcc gcacgctatc
     6121 tcgaagcgct gcgcctgctc gggacgccgg ccatcccgca gagccgcctc tggcgcctgc
     6181 ccgcgccggc ggccgctgcg gccgagaacg agaccacctg gcagcgaagc gccgacggcg
     6241 ccctcctgga caccgtcatg cagagcgcct cgctgcagca ggtgcccttt ctgctgctcg
     6301 gggccgagga caccggcgcc gtgctgagcc gcctgcgcag catcaccgca gcgctgttcg
     6361 ggttgcagac cttcgccgct ttcctccatg atcccgccgc acgctccttg aacggatggc
     6421 tggtcaccgt ggaccggctg gcgcccaccg agctggagat cccggtcacc ggcggcacca
     6481 gcctggtggc gcgcgccgcg gccgagcgcc aggcgctgcg cacgcaaggc ggcgagggcg
     6541 gcgagcgctt gcgcggcgtg gacctggaga tcggccggct gctgcaggcc gacgcgttga
     6601 gcatcgtgcc gctgatcgcc ggcggcaacc tgctgggcgt gctggtgttc ggagtggggc
     6661 gtgagcaggc gccgcggctg gcgggcgact cgctgatgct gcagcggctg tccgagatgg
     6721 caaccctggc gctcaccaat cggcgccaga gcgccgagca gcagcgcttg aaggaagcgg
     6781 acctgcgcga gcggctgcgg ctgtcggcgc gccgtctggt gcacgaggcg cgcaatccgc
     6841 tgtcggtgat gaagacgcac ctcgagctgc tgggtgaacg cgtccagagc gggcagccga
     6901 tcgacaagga cctgcgcgtg ctgcgtgagg agatcgatcg cgtgggcgag atcgtggcgc
     6961 gcatcggcac caccgatttg tcggtggaaa agggccatgc gccggtggac atcaacggtt
     7021 tgatccgcga gctgatgatc gtgtaccgcg aagcgttgtt cgccacccgc ggggtgaacg
     7081 tggagctgga gctcgacccg cgcctgccgg gcatcgtcac cgacggcaac gcgctgaagc
     7141 aggtgttgct caacttctgg aagaacgccg ccgaggcgat gcccgcaggc ggacaggtgc
     7201 gggtgcgcac cgcggaccgg gtcaactacg agggtcaact gatgctggag atcacggtat
     7261 ccgacaccgg cagcggcatg ccgccggagg tgctggagaa tgtgttctcg cagcgcctga
     7321 cgctcaccaa gggatccgag cgcggctacg ggctgagcaa cgccttcacc ctggtcaagc
     7381 aactgggcgg ccatgtcctg tgccgcagcg agcccgagcg cggcaccctg ttcaccgtgc
     7441 tgctgccgcg cacgctgcac gcgcccggga cctgagatgc agggcatgcc ggacttgggc
     7501 cttccgccag ccgacgctcc cggcctctac ctgcccgggt cggaggcgat cgcggagggc
     7561 gcgcgcatcc ttctggtgga cgacgaaccg cgcatccggg aagcggtgga ggcgctgatc
     7621 gctcagcccg gccgccagat cctgcactgc ggcagcggcg gcgaagcgct gcgcatcctg
     7681 ggcggcgggc aggtcgacct ggccctgctc gatatccggc tgccggacat gaccgggctg
     7741 gagatcctgg agcgggtgcg gcgcgaaggc tcgtcggtgc gcgtgatcat cgtcagcgcc
     7801 gacgaccagg tggactcggc catcgccgcg ctgcgcttcg gggcctacga gtacgtacgc
     7861 aagccgttcg agccggcggc cctgcgccgc accgtggaca acgcgctgtc gcagctgcgc
     7921 ctggagcgcg acaacgagcg catgcgcggg cggctcgagc agtcggagcg actgcatcgc
     7981 tacctcatcg accagtcgcc cgatctgatc tacacgctgg accgggacgg gcgcttcacc
     8041 ttcgtcaacg agcgcttcca tcgcctgctc gggttcgagc gcgacgagct gatcggcacg
     8101 cactactcgt ccatcgtcta ccgcgaagac gtcaagcgcg ccgagtacgc gttcaacgaa
     8161 cggcgcaccg ggcagcgtgc ctcgagcaac gtcgagctgc gcgtcacgcg caaaggcgga
     8221 gacggcacgc cctgcaccat cgtgctgagc gcggtcgggg tgtacgcgcg acgcgaggcg
     8281 gggagcgacg cccgctcggg gttcgtcggt acctacgggg tggggcgcga catctccgac
     8341 cgcaagcgcg ccgaggagat cgtcagctac cacgccttct acgacgtgct caccgggctg
     8401 cccaaccgca ccctgttccg cgaccgcctg cgccaggcca tcgcgcaggc acgccggcgc
     8461 cgctcgcgcg tggcggtgat gttcatcgac ctcgaccgct tcaagctggt gaacgacacc
     8521 tacgggcacg tgcgcggcga tctgttcctg caacaggtcg ccgggaggct gcggcgccag
     8581 ctgcgcagca gcgacacgct ctcccggctc ggcggcgacg agttcacggt gctggtgccc
     8641 gacctgagga cgcccgagga cgcggcgttg aaggcccagc agttcctgga ggcgctgcgg
     8701 cgtccgttcg tggtcgaggg cgaggagctg gcggccaccg ccagcatcgg cctggccgtg
     8761 ttcccggacc acggtgccag cgaggacgag ctggtgcgca acgccgatct ggcgatgtat
     8821 caggtgaagc gccgcggcaa ggacggcgtg ggcttcttcg ccaaggagat gagcagcctg
     8881 tacagcgaga agatcaatct ggagggcgag ctgcgcaagg cgctgcagcg cgccgacttc
     8941 caggtgctct accagccgat ccacgatgtg cgcaagggca gcgtggaatg cgtcgaggcg
     9001 ctgttgcgct ggcagcaccc gacgctgggc acgctggagc cggcgcgctt catctacatc
     9061 gccgaagaga ccggcatgat ctcgctgctc tccgatcgcg tgctcgattc ggcctgcgcc
     9121 cagctcaagc agtggcgcgc ggagggtttc cacaagctgc gcgtggcggt gaacgtctcg
     9181 gcgcgcgact tcgaacgcag cgacttcacc gagcgcgtgg tgcaggcgct gacccgccac
     9241 aagctgcagc ccagcgcgct cgagctggag atcaccgaga acgtgctgat cgaggacgtg
     9301 gacgcggcgg tcgacaaaat ccgtcagcta cgcaagaccg gcgtgcgcat ctcgatcgac
     9361 gacttcggca cccgctattc ttcgctcggc tacctgcagc gtctgccggt gaacaccatc
     9421 aagatcgatc aggtgttcac gcgcgagctg gacgaggcgc agccgcagag ccctatcgtg
     9481 ctggccatcg tcggcatcgc caagggcttc gggttgcacc tggtggccga gggcgtggag
     9541 cgcgccgtgc accgcgacat gctgcgcaac ctcggttgcc agagcatgca gggcttcctg
     9601 ttcagcccgg cgctgaccgc cgaggaaacc accagctacc tgcggcgcgc gcacgacggc
     9661 acgctgtgac gccggcgtcc tcggctacac gttgaagcgg aagtagacga cgtcgccgtc
     9721 gcgtaccacg tagtccttgc cctccagccg catcttgccc gcctccttcg cgccctgctc
     9781 gcccttgagc gcgacgtagt cgtcgaaggc gatgacctcg gcgcggatga aacccttctc
     9841 gaagtcggtg tggatcacac cagcggccat gggcgcggtg tcgccccggt ggacggtcca
     9901 ggcgcgcacc tcttttggtc cggcggtgaa gaaggtctgc aggcccagca gctcgtaggc
     9961 cgcgcggatc aggcggttca gccctggctc ttccagcccg aggtcggcga ggaagacgcg
    10021 cttgtcctcg tcggacagat cgacgatctg cgcctcgagc gcggcgcaga tcgcgaccac
    10081 cggcgcgcct tcgccgcgcg cgaactgcct cacacgctcc agcagcggat tgcgctcgaa
    10141 gtccttctcg gccacgttgg cgacgtacat ggtcggcttc atggtgagca ggaacagcgg
    10201 ccggatcagc tccgtctctt cggggtagag cggcaccgag cgggccgggc gcgcctggtc
    10261 gagcgcgggc tgcaccttct ccaggaccgc gaccagccgt tgcgcttctt tgtccccgcc
    10321 ggcctgggcc accttcgagt acttggcgag ctgcttctcg accgtggcca ggtcggcgag
    10381 cgccagctcg gtgttgatgg tctcgatgtc cgataccgga tcgaccttgc ccgccacgtg
    10441 caccacgttg ggatcctcga agcagcgcac cacgtgcgcg atggcgtcgg tctcgcggat
    10501 gttggccagg aactggttgc ccaggccttc gcccttggac gcaccggcca ccagcccggc
    10561 gatgtccacg aactcgacga ccgccgggat cgccttctgc ggcttggcga tcgcggcgat
    10621 ccgctcgagc cgcgcatcgg gcaccgccac gatgccgacg ttgggctcga tggtgcagaa
    10681 cggatagttc tccgccgcga tgccggcctt ggtgagcgcg ttgaacagag tggacttgcc
    10741 cacgttgggc agtcccacga tgccgcattt catgcgagaa acgtgattcg tgactcgtga
    10801 ctcgtgattc gtgattcgtg actcgctgtg actcgcgatt cgtgattagt actcgcgccg
    10861 cttgaccggg gaggagcaac aagctcgcga tccacgattc acgaatcact gtctctagtg
    10921 tgcagccggt gcatcgcctt ctccatctcg ccggcgaata tcagcggcca cacctccagc
    10981 gagcgctcga cggcatccga gatcagccgc gtctcttcct cgcgcggcgg acgcagcacg
    11041 tagttgacca cctcggcgcg ctcgccggga tggccgatgc ccagccgcag gcgccagaac
    11101 tccggcccga gatggcgcgc gacgtccttc aggccgttgt ggccggccac gccgcccccc
    11161 ttcttcagac gcaccgcccc cggaggcaga tcgagctcgt cgtgcaccac cagaatctct
    11221 tccggcgcga tcttgtagaa gccggccagc gccgcgaccg accgaccgct gtcgttcatg
    11281 taggtctgcg gcagcagcag ccagcagtcc tttccgtgcg cggcgatccg ccccaccctg
    11341 ccgtggaagc gcgactcggt cttgagcgac acgcgctcga tctcggccag gttctctagc
    11401 caccagaagc cggcgttgtg gcgcgtggtc tcgtactcgc gccccggatt gcccaggccc
    11461 accaccagtc tcatgaacgg gtcgtactct caaacgagaa ggcccgcgcg gctgcgcggg
    11521 cccccggttc gcgtgcagcg gcgagaggct atttctttct ctcccggtcc ttcctctctt
    11581 ccttcttctc cttctcctcc ttctcctttt cgccttcggc cttctccggc ggcgctccag
    11641 gcactgcggc ggccggggcg gctaccgctg tagtcaccgg ggcgacctcc tcctcgacca
    11701 ccggctcggc cttcggcacc gggatggtga ccaccaccgc gtcgtcgccg ccgcgcagct
    11761 gcgacgactc cacgcccttg ggcagcttca gctccgacag gcggatcgaa tggcccatct
    11821 gcaggttgga gaggtccacg tcgatgtact cgggcaaatc gtcgggaagg caggtaacgt
    11881 cgatctcggt aagcacgtgg ttcaccgagc caccgccggt cttcaccccc ggtgccacct
    11941 cggcgttgac gaagtgcagc ggtatcttca tgtggatctt gcggtccttg gccacgcgct
    12001 ggaaatccac atgcagcacc tgctgccgcc aggggtgcat ctgatagtcg cgcagcagca
    12061 ccggcagctt gctgccttcc atgttcagcg tcaggatcga ggcgtggaag gtctcgtgct
    12121 tgaggtgctg cgccagctcc ttgtgatcga cctcgatgct ggcggccggt tgcccgccgc
    12181 cgtacaggat acccggcacc cgttgcgcat gacgcaggcg gcggctcgca cccttgccct
    12241 gcgtcgaacg cggggttgcg ttcacttcga tcggcattgc tctacctccg gtcagtatcg
    12301 ccgcccgcga ccaggcggcg gtgtcgataa tgcgtgcttc gtgactcgtg attcgtgatt
    12361 ggaatcaccg cttccgtacg aatcacgatt cacgaatcac gaatcacgta tttcccatca
    12421 gtccatgaac agcgagctca ccgactcctc gttgctgatg cgcagcatgg tctcggccag
    12481 cagctcgccc accgccagct ggcggatgcg cttgctggcg cgcgcgtcgc cgttcagcgg
    12541 gatggtgtcg gtgaccacca gctcgtcgat ctccgagttc tcgatgcgct gcaccgcatt
    12601 gccggagagc accgggtgcg tgcagtacgc cagcacccgt tcggcgccct tctccttcaa
    12661 cgcgagcgcg ccctcgcaca gggtattggc ggtgtccacc atgtcgtcca tgatgatgca
    12721 ggtgcggccc ttcacctcgc cgatgatgtt catcaccgtg gcgacgttgg gccgcggccg
    12781 gcgcttgtcg atgatggcca gatccgagtc caggcgcttg gccagagcgc gcgcgcgcac
    12841 cactccgccg acgtccggcg acaccaccac caggttctgg tggccatgct tccatacgtc
    12901 gcccagcagg atcggcgccg cgtagatgtt gtccaccggg atgtcgaaga agccctggat
    12961 ctggtccgag tgcaggtcca tcaccagcaa acggtccacg cccaccgcct gcagcatgtt
    13021 ggccaccacc ttggcgctga tcgcgacacg cgccgagcgc gggcggcggt cctggcgggc
    13081 gtagccgaag taggggatgg cggcggtgat gcgctcggcg gaggagcgct tgagcgcgtc
    13141 caccatcagc atcacctcca tcaggttgtc gttggtgggc gtgcaggtgg actgcagcac
    13201 gaacacgtcc ttgccgcgca cgttttcgag caactcgacc atcacctcgc cgtcgctgaa
    13261 gcggctcacg gtggcgcgcc cgagatggat gttcaggtgc cggacgacat cctgcgccag
    13321 cgccgggttc gcgttgccgg tgaacaccat caagctgtcg taggccatgg acggaagggt
    13381 ctaggagcta ggatctgggg aacacagtgc gaagataacg caagcgga
//
LOCUS       DASPGE010000414         4920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_464182, whole genome shotgun sequence.
ACCESSION   DASPGE010000414 DASPGE010000000
VERSION     DASPGE010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464182"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VD791_13640"
     CDS             complement(<1..328)
                     /locus_tag="VD791_13640"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV09071.1"
                     /translation="MHRIRIDTLMLWLLAAALALAASAVFAGAREIRGFALTVSDLDR
                     AVAFYEQALGFRKVGERTFAGPDQDALTGIFGTRVRMARLQLGDEFVELEQYVAAAGQ
                     PIPLDSR"
     gene            complement(339..1178)
                     /locus_tag="VD791_13645"
     CDS             complement(339..1178)
                     /locus_tag="VD791_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV09072.1"
                     /translation="MNAISRTERQALSLRPLEGQRAIVTGANSGIGEGVAKALAEAGA
                     RVVVNYVAGPDRAEQVVDEIRRAGGEAVPVRADVSREDQVEAMFAQTIAAWGSVDIVV
                     NNAGLQRDAPLHQMSLKDWQFVLDVNLTGQFLCARAAVREFLRRGVVPELSAAAGKII
                     CMSSVHDVIPWAGHANYAASKGGVSMLMKTMAQELAMHKIRVNAVSPGAIKTPINTDA
                     WATPEAEQKLLKLIPYYRVGETHDIARAVVWLASDLSDYINGATLYVDGGMTLFPEFR
                     GNG"
     gene            complement(1197..1679)
                     /locus_tag="VD791_13650"
     CDS             complement(1197..1679)
                     /locus_tag="VD791_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXV09073.1"
                     /translation="MEFVARSVGGMRVTDAVFATARVIIALLERFLAPVLDLAIRLWV
                     GTAFFQSGLAKIQSWDTTLALFENEYSVPLLPPVLAAYLGTATELIVPVLLIVGLGGR
                     FAAAVLFVFNIVAVISYGDLSEAGLMQHQIWGLLLLVTLLHGPGKLSVDHLIRRRFLG
                     "
     gene            complement(1813..2106)
                     /locus_tag="VD791_13655"
     CDS             complement(1813..2106)
                     /locus_tag="VD791_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HXV09074.1"
                     /translation="MNASDRILRAAVASLLAIGVSAASQQALAAKGDNEKCAGVVKAG
                     KNDCGTSRNACSGQVKTDNDPEAWIYLPKGLCERIVGARVQMSSHAKPGGKKG"
     gene            complement(2240..3037)
                     /locus_tag="VD791_13660"
     CDS             complement(2240..3037)
                     /locus_tag="VD791_13660"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HXV09075.1"
                     /translation="MRSLRELQSGFAAALLEPIAGRAAPGIRADGISPALRLGFYRTN
                     VLENYRKALAATYPAVERLVGIGCFLNLSSEYARRHASRSGDVGMHGERFADFLERHA
                     VGRALPYLADVARLEWAIEESFYEADREALSLDRLARVPAEQVSALRFHLAPSARLLR
                     SRFPVYRIWEISQPDYTGDEPVDLDSGAMQLLVRRERYAVALDPLPAGEYAMLEALRA
                     GYGFGEAFACALTVDGELDAGAFLRRHIASGVLCDFTLPAEAHAPAP"
     gene            complement(3021..3920)
                     /locus_tag="VD791_13665"
     CDS             complement(3021..3920)
                     /locus_tag="VD791_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HXV09076.1"
                     /translation="MNAIENRRRGRAAIPVRVGIGLRPPHYRQLLSELPAVGWLEVHS
                     ENYFSRGGQPLHFLARARGHYPLSLHGVGLSLGSADALSTDHLMHLKRLIERLEPGAV
                     SDHLCWGAIGGRHLNDLLPLPYTDEALQLVCAHIDEAQAFLGRELLIENVSSYLQFSA
                     SVIPEWEFLAAVARRTGCGILLDVNNIYVSSVNHGFDPLRYLEAMPDGAVQEVHLAGF
                     DTVPVAGGTGGSVLIDTHGKRVAEPVWNLYREALSRLGAVPTLIEWDTDIPPLATLLE
                     EARKAQEILDRWSLAEAVDALAA"
     gene            complement(3917..4105)
                     /locus_tag="VD791_13670"
     CDS             complement(3917..4105)
                     /locus_tag="VD791_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV09077.1"
                     /translation="MSRLSEAALDALAQGLPPPSAAADAVEIEPAPWVSLTKAQCLLL
                     AIALHRVARARTSGGKVP"
     gene            complement(4161..4358)
                     /locus_tag="VD791_13675"
     CDS             complement(4161..4358)
                     /locus_tag="VD791_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV09078.1"
                     /translation="MSSIALHADPFLPQFVPATRTASALQSLMRMWEQEAPAEEEESE
                     TELMVMLSGMVGSHATSLGDE"
     gene            4690..>4920
                     /locus_tag="VD791_13680"
     CDS             4690..>4920
                     /locus_tag="VD791_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV09079.1"
                     /translation="MHLAARVPGEPDAAVPCILRERRARRLHRGGDALLLGDLCRVAA
                     GVRTGMRQEHDNQRTGQADFHLHIPRLETGGTP"
BASE COUNT          802 a         1661 c         1687 g          770 t
ORIGIN      
        1 agcggctgtc gagcggaatc ggttgcccgg cggcggcgac gtactgctcc agctcgacga
       61 actcgtcgcc gagctgcagg cgcgccatgc gcacgcgcgt gccgaagatg ccggtaagag
      121 cgtcttgatc ggggccggcg aaggtgcgct cgcccacctt tctgaatccc agcgcctgct
      181 cgtagaacgc caccgcgcgg tcgagatcgg acaccgtcaa ggcgaagccc cggatctcgc
      241 gcgcgccggc gaacacggcg ctggcggcca gtgccagggc ggcggccagc agccagagca
      301 tcagggtgtc gatgcgaatt cggtgcatgg tcttctcctc acccgttccc gcgaaattcc
      361 ggaaacagcg tcatcccgcc atccacgtac agcgtcgcgc cgttgatgta gtccgacaga
      421 tccgaggcga gccataccac ggcgcgcgcg atgtcgtgcg tctcgcccac ccggtagtag
      481 ggaatcagct tgagcagctt ctgctcggct tccggcgtgg cccaggcgtc ggtgttgatc
      541 ggcgtcttga tggcgccggg cgacacggcg ttgacgcgaa tcttgtgcat cgccagctcc
      601 tgagccatgg tcttcatgag catcgacacc ccgcctttcg aggccgcgta gttggcgtgc
      661 ccggcccagg gaatgacgtc gtgcaccgag gacatgcaga tgatcttgcc ggcggcggcg
      721 gacagctccg ggaccacgcc gcggcgcagg aactcgcgca ccgcggcgcg cgcgcacagg
      781 aactggccgg tcaggttgac gtcgagcacg aactgccagt ccttcaggct catctggtgc
      841 agcggcgcgt cgcgctgcag gccggcgttg ttcaccacga tgtccacgct gccccaggcg
      901 gcgatggtct gcgcgaacat cgcctccacc tggtcctcgc ggctgacgtc ggcccgcacc
      961 ggcaccgctt cgccgccggc gcggcgaatc tcgtccacca cttgctcggc ccggtccggg
     1021 ccggccacgt agttgaccac caccctggcg ccggcctcgg ccagcgcctt ggccaccccc
     1081 tcgccgatgc cggagttggc gccggtgacg atcgcgcgct ggccttcgag cggacgcagg
     1141 ctcagcgctt ggcgttcggt cctgctgatc gcattcatgc gcgttctccg gtcgaatcag
     1201 ccgaggaagc ggcggcgaat caggtggtcg accgacagct tgcccggccc gtgcagcagc
     1261 gtcaccagca ggagcagccc ccagatctgg tgctgcatca agccggcttc ggacagatcg
     1321 ccgtaggaga tgaccgccac gatgttgaac acgaacaaca cggcggcggc gaagcgcccg
     1381 cccaagccga cgatcaacag caccgggacg atcagctcgg tggcggtgcc gaggtaggcg
     1441 gcgagcaccg gcggcagcag cggcacgctg tactcgttct cgaacagggc cagagtggta
     1501 tcccagctct ggatcttggc gaggcccgac tggaagaagg ccgtgcccac ccacaagcgg
     1561 atcgcgaggt cgaggaccgg ggcgaggaag cgttcgagca gcgctatgat cacgcgcgcg
     1621 gtggcaaaga cggcatcggt gacgcgcatg ccgccgacag atcttgcgac gaattccatg
     1681 gtggttatcc ttgatcgatt gaccgcatcc gcgcggccgg gatcgcgagt cgcgactggc
     1741 gcgctgcgcg cggatgcggt cgagcttgct agtcgccgat ttgcgccgtc tgggcgcagg
     1801 aaacggtcgg cttcagccct tcttgccgcc cggcttggcg tggctggaca tctgcacgcg
     1861 cgcgcccacg atgcgctcgc acaggccctt cggcaggtag atccaggcct ccggatcgtt
     1921 gtccgtcttc acctgcccgg agcaggcgtt gcggctggtg ccgcagtcgt tcttgccggc
     1981 cttgacgacg ccggcgcact tttcgttgtc gcccttggcg gccagcgcct gctggcttgc
     2041 ggcggaaacg ccgatggcca acagcgaggc gacggcggct cggaggatgc gatcggatgc
     2101 gttcatgctt tgactcctgg gagtgaaatg gtcgatgcga atgattcgct ggtgttccag
     2161 acgtcagctc ggctgacctg gtgacgcgca gtatcggctg cagttctcac atcgatctca
     2221 cgggcgccta aagcttcgct cacggcgccg gcgcatgcgc ttcggccggc agcgtgaaat
     2281 cgcacaacac accggatgcg atgtgccggc gcaggaaggc gccggcgtcc agctcgccgt
     2341 cgacggtcag cgcgcaggcg aaggcttcgc cgaaaccgta gcccgcgcgc agcgcctcga
     2401 gcatggcgta ctcgccggca ggcagaggat ccaaggcaac ggcgtagcgc tcgcgtcgca
     2461 ccagcaactg catcgcgccg gagtcgaggt ccaccggctc gtcccccgtg tagtccggct
     2521 ggctgatctc ccagatgcgg tacaccggaa agcgcgagcg cagcagccgg gccgagggcg
     2581 ccaggtgaaa gcgcagcgcg gacacctgct ccgcgggcac ccgcgccagc cggtcgagtg
     2641 acagcgcctc ccggtcggct tcgtagaacg attcctcgat cgcccattcc agccgcgcga
     2701 catccgccag atagggcagc gcgcggccga cggcgtgacg ctcgaggaag tcggcgaagc
     2761 gctcgccgtg catgccgaca tcacccgaac gcgacgcgtg ccggcgcgcg tactcggaag
     2821 acaggttcag gaagcagccg atccccacca gccgctcgac ggcgggatag gtggccgcca
     2881 gcgccttgcg atagttctcc aacacgttgg tgcggtagaa gccgaggcgc agagccgggc
     2941 tgatgccgtc ggctcggatc ccgggcgcgg cgcggccggc aattggctcg agcagcgcag
     3001 cggcgaaacc ggattgcagc tcacgcagcg agcgcatcga ccgcctccgc caggctccac
     3061 cggtccagga tttcctgcgc cttgcgcgcc tcctcgagca gcgtggcgag cgggggaata
     3121 tcggtgtccc actcgatcag cgtcggcacc gcgcccaggc gcgaaagcgc ctcccggtac
     3181 aggttccaca ccggttcggc gacgcgcttg ccgtgcgtgt cgatcagtac gctgccgccg
     3241 gtgccgccgg ccaccggaac ggtgtcgaag ccggcgaggt ggacttcctg cacggcgcca
     3301 tcgggcatcg cctccagata gcgcagcggg tcgaaaccgt ggttcacgct gctcacgtag
     3361 atgttgttga cgtccagcag gatgccgcaa ccggtgcggc gcgccacggc ggccaggaac
     3421 tcccactcgg gaatgacgga agccgagaac tgaagatagc tggagacatt ctcgatcagc
     3481 agctcccggc cgaggaacgc ctgggcctcg tcgatgtgcg cgcagaccag ttgcagcgct
     3541 tcgtcggtgt agggcagcgg cagtagatcg ttcaagtgac gtccgccgat cgcgccccag
     3601 cacagatggt cggaaaccgc gccgggttcg aggcgctcga tcaagcgctt gagatgcatc
     3661 aggtgatccg tcgacagcgc gtcggccgac cccagcgaca ggcccacgcc gtgcaagctc
     3721 aacgggtaat gaccgcgcgc gcgcgcgagg aagtgcagcg gctgcccgcc gcgcgagaaa
     3781 tagttctcgc tgtgcacttc cagccagccc acggcgggca gctcgctcag cagctgccgg
     3841 tagtgcgggg gccgcagacc gatgcccacc cggaccggga tcgcggcgcg gcctctgcgt
     3901 cggttctcga tcgcgttcat gggaccttcc ctccgctggt gcgtgcgcgc gccacgcgat
     3961 gcagcgcgat ggccagcagc aagcactgcg ccttggtcag cgacacccag ggcgcgggct
     4021 cgatctcgac ggcatccgcg gcggcggacg gcggcggcag gccctgtgcc aaggcgtcca
     4081 gcgcggcttc ggacagccgg ctcatcaggc gatccgggcc gctcgacggt ggcgcggcag
     4141 aggtcatgcg ccgtcccggc ttattcgtcg cccaggctgg tggcatggct gccgaccatg
     4201 ccgctcagca tcaccatcag ctcggtttcg ctttcttctt cttcggcggg cgcttcctgc
     4261 tcccacatcc gcatcaggga ctgcagcgcg ctggcggtgc gcgtggcggg cacgaattgc
     4321 ggcaggaacg ggtcggcatg caaggcgatg ctgctcatgt tgggctcctt tggatcgagg
     4381 tctcttgcga acacggagcc gcagtatcga ggccggtgat cacgcaatct tcacggccca
     4441 ctaaatcttt gctcacgtcg cgcggcccgt ttaacgcgtc gcgccgtggt cgtaaggttg
     4501 cccatagtgc gccgactaag tgcgtttggg ccgcgtagcc cggatgcagc gcagtggaat
     4561 tcggggaagg gaaggcggcg cggcagcact gcgaaaagag agatgggcgg ggcttgcgct
     4621 gggcgcgcag cccgcaaccc atcttaaggg ggaggagact ggacggaggc taaactctta
     4681 ctgcactcga tgcacctggc cgcgcgcgtc ccaggtgagc cggacgcggc cgtgccgtgt
     4741 atcctgcgcg agcgccgtgc gcgccgcctt caccgcggcg gcgatgcgct gctgctcggc
     4801 gatctgtgcc gcgtcgcggc cggcgtacgc accgggatgc gccaggagca cgacaaccag
     4861 agaacaggcc aggccgactt tcatctgcac atccctcgtt tggaaaccgg cggcacaccg
//
LOCUS       DASPGE010000415         7857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1047526, whole genome shotgun sequence.
ACCESSION   DASPGE010000415 DASPGE010000000
VERSION     DASPGE010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7857
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047526"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1166
                     /locus_tag="VD791_13685"
     CDS             111..1166
                     /locus_tag="VD791_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXV09080.1"
                     /translation="MTTTTRWKRRPEGSTWGDFGPDDQLGRLNLLTPQKVKQGVAEVK
                     EGLTFCLSLPLDFPGGNVLNPRRHPPVLRPTLRNGRPNMNYQLGTDNPSLTDVINDDA
                     VIMHLQYSTQWDSLAHVGQMFDANGDGVPEPVYYNGYRPGEHIVGPSAPDDAGAIGQV
                     PARSTSRAKALGVENMAAKCVQGRGVMIDLHAHVGRSRAVIGYDRLMRILEADRVVVE
                     QGDMACFHTGFAQMLLEMERKPDGHKLENACAALDGRDRRLLQWITDSGVAAMIADNY
                     AVEAHPPTSMGERCAYLPLHEHCLFKLGVNLGEIWYLTELADWLRKNGRCRFLLTAPP
                     LRLPGAVGSPTTPVATV"
     gene            1177..2310
                     /locus_tag="VD791_13690"
     CDS             1177..2310
                     /locus_tag="VD791_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXV09081.1"
                     /translation="MSYLSGVGLTPYGKLPGSSTLDLMSAAAGAALADAQLERKDIDG
                     LLCGYSTTFPHLMLSTVFAEHFGLEPAYAQAIQMGGATGFGMAMLAHHLVDAGLARNV
                     LVVGGENRLTGQGRDAAVQTLAQVGHPVYEVPLGPTIPAYYGLVASRYLHEHGTTEHD
                     LAELAVLMRRHASTHPGAQFKEPITVADVLASKPVAAPLKLLDCCPVSDGGAAFVLSR
                     EPTGASRVRVAGTGQAHTHQHVTAALSLTQFGAGASARRAMQAAHVGLDEVKYAAIYD
                     SFTITLTILLEEIGLAPRGQAGRLAREGRFSRDGRFPLNTHGGLLSYGHSGCAGAMAH
                     LVEAQLQMTRRAGARQVRDASVALLHGDGGVLSTHVSLVLANP"
     gene            2336..2683
                     /locus_tag="VD791_13695"
     CDS             2336..2683
                     /locus_tag="VD791_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HXV09082.1"
                     /translation="MIPDWTQGGEGIAYQACVGCGAVWYFRRTFCPRCGRASPEDRAA
                     GGAGTVHAVTLVARAPSEELRAHAPYAIVLVDTDEGFRMMAHGDVDLRIGDRVQCRFI
                     SLAGRIVPRYDKR"
     gene            2786..4897
                     /locus_tag="VD791_13700"
     CDS             2786..4897
                     /locus_tag="VD791_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HXV09083.1"
                     /translation="MTDSRRAALRTVLDPRSIAVIGASENPNKIGGRPLLYLRKFGFR
                     GKVYPINPNRKEVQGFKAYPGLAALPETPEVAIVVVPDDLAVQAVDDCAARGVKTAIV
                     MASGFGETSDPAAQDKERRMAERGRAAGMRIVGPNTQGLANFASGAVLSFSTMFLETE
                     ARDGPVGVVSQSGAMSVVPYGLLRSRGVGVRYACATGNDCDVSVGELASVVAEDAQLK
                     LLLLYLEAIRDPAPFVELARIARARGLPIVALKSGRTPAGQRAAQSHTGALANEDRVV
                     EAFLERHGIWRARDVNELVDGAELYLKGWKPKGRRLVAISNSGASCVMAADAATEAGL
                     ATPALSDETRAGLSRILPGFATTTNPVDITAALLSNSSLFGQILPVIARDPAADAFFI
                     AIPVAGQGYDVDTFASDAAVFARRTGKPVVTAIPQASIAAKFKAQGLPVFGTDAEAVR
                     ALGRYLAHVELMQRIAAEPSRAAPMRRPAPPNWHALNEADSLERVRRHGVPVVEHRVC
                     RTPDEAQAALAELGGPVAVKGCSRDVPHKSEWGLVKLGLTTADQVRSAYSDFDKTLRI
                     AGGRFDGLIVARLVKGLRELMIGAHLDPVFGPVVVVGDGGKYVEAMPDVQVLLAPFTP
                     DDVFRALQRLRIAPLLAGVRGDPPLDVDEFCNAAARVGAMMAAADSDVVSLDLNPVIV
                     RAQREGCEAVDALVVVASPPA"
     gene            4938..6329
                     /locus_tag="VD791_13705"
     CDS             4938..6329
                     /locus_tag="VD791_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HXV09084.1"
                     /translation="MPAYAHTVGSLTYRFADLKTLMAKATPARTGDGLAGIAAASAEE
                     RVAAQMALADLPLTAFLAEHLVPYEEDEVTRAILDHHDVRAFAPVAHLTVGELRNWLL
                     SYEADEAALAALAPGLTPEMVAAVCKLMRIQDLVLAAAKCRVVTRFRNTLGLRGRLSS
                     RLQPNHPTDDPKGIAASIVDGLLYGCGDAVIGINPATDNLEQACALLALLDDIIAGHR
                     IPAQSCVLTHVTTTLRAIERGAPVDLVFQSIGGTEATNRSFGVSAALLDEANRAAQGL
                     ARGGPGANVMYFETGQGSALSANAHHGVDQQTLEARAYGLARLYRPLLVNSVVGFIGP
                     EYLDGREIVRAGVEDHFCGKLLGLPMGVDVCYTNHADADQDDMDTLLTLLGAAGVNYI
                     MGVPGADDIMLNYQSTSYHDALYLRQALGLRPAPEFEDWLMRMGVFDTPTRLRPADPR
                     HPLLGLVGGRGAE"
     gene            6349..7164
                     /gene="eutC"
                     /locus_tag="VD791_13710"
     CDS             6349..7164
                     /gene="eutC"
                     /locus_tag="VD791_13710"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HXV09085.1"
                     /translation="MPRSPKTALGRPDPWQALNRFTAARIGLGRAGGSLPTRPLLEFQ
                     LCHARARDAVQRDLDTVAQEQRLRECGFEVLQLASAAADRHAFIRRPDQGRILHPDSR
                     ALLERRPPRAEPYDAVFVLADGLSALAVERHATPLLERIAPRLIDAGWHLAPVCVVRQ
                     GRVAVADEIGTLLPACMSVMLIGERPGLTSPDSLGIYLTWDPLPGRSNAERNCISNVR
                     PEGLDYAHAAHKLFYLMSESRRRRLSGVDLKEDAPALPAAGSGALPGAGSGPR"
     gene            7416..7826
                     /locus_tag="VD791_13715"
     CDS             7416..7826
                     /locus_tag="VD791_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HXV09086.1"
                     /translation="MAQAKKARKKTTARKPARKAPAKKPAKRKAAPKKAPTRKAARKP
                     AKKAAPKRKRTPNAAFMKAMTPSGDLAAVVGDRAMPRTEVTKKIWDYIKRNKLQDSAN
                     RRMINADDKLRPVFGGKRQVSMFEMTKLVNKHLK"
BASE COUNT         1216 a         2752 c         2761 g         1128 t
ORIGIN      
        1 ggcgcgacgc tcgccgatca tcttccgaac cgaagcgccg cgaatcgcgg caggttccgg
       61 tctgcgcggc cgtcgcgtcc cggcgatgcg aagcgctcga gggggagaca gtgactacca
      121 ccacacgctg gaagcggcgt cccgaggggt ctacgtgggg cgatttcggt cccgacgatc
      181 agctcggccg gctaaatctg ctcacgccgc agaaagtgaa gcagggcgtg gccgaagtga
      241 aggaggggtt gacgttctgc ctcagcctgc cgctggattt cccgggcggc aacgtgctga
      301 acccgcgccg gcatccgccg gtgctgcggc cgacattgcg caacggccgg cccaacatga
      361 actaccagct cggaacggac aatccgagcc tgaccgacgt gatcaacgac gacgccgtca
      421 tcatgcacct gcagtactcg acgcagtggg acagcctcgc ccacgtcggg cagatgttcg
      481 acgccaacgg cgacggcgtg ccggaaccgg tgtactacaa cggttaccgc ccgggcgaac
      541 acattgtggg cccgagcgcg ccggacgacg ccggcgccat cggccaggtg ccggcgcgct
      601 cgacttcgcg cgccaaggcg ctcggggtcg agaacatggc cgcgaagtgc gtacaggggc
      661 gcggggtgat gatcgacctg catgcgcacg tcgggcgcag ccgcgccgtg atcggctacg
      721 accggctgat gcgcatcctg gaagcggacc gggtggtggt ggagcagggc gacatggcct
      781 gcttccacac cgggttcgcg cagatgctgc tggagatgga gcgcaagccc gacggccaca
      841 agctggagaa cgcttgcgct gcgctcgacg ggcgcgatcg gcggttgctg cagtggatca
      901 ccgacagcgg ggtggcggcg atgatcgcgg acaactacgc cgtcgaagcg catccgccca
      961 ccagcatggg cgagcgctgc gcctacctgc cgctgcacga acactgcctg ttcaagctcg
     1021 gcgtgaacct gggcgagatc tggtacctga ccgagctggc cgattggctg cgcaagaacg
     1081 ggcgctgtcg cttcctgctg accgccccgc cattgcggtt gcccggcgcc gtcggttcac
     1141 ccaccacgcc ggtagccacg gtgtagcacg cgtggcatga gctacctgag cggagtcggc
     1201 ctcacgccct acggcaagct gcccggctct tccacgctcg acctgatgag tgcagcggcc
     1261 ggcgccgcgc tggccgatgc acagctcgag cgcaaggaca tcgacggcct gttgtgcggg
     1321 tactcgacca cgttcccgca cctcatgctg tcgaccgtgt tcgccgagca tttcgggctc
     1381 gagcccgcct atgcgcaggc gatccagatg ggcggcgcca ccggcttcgg gatggcgatg
     1441 ctcgcgcacc atctggtgga cgcgggcctc gcgcgcaacg tcctcgtggt gggcggtgag
     1501 aaccggctga ccggccaggg ccgcgacgcc gcggtccaga cgctggcgca ggtgggacat
     1561 cccgtctacg aggtgccgct ggggccgacc atcccggcct actacggact ggtcgcctct
     1621 cgctacctgc acgagcacgg tacgaccgag cacgatctcg ccgagcttgc ggtgctgatg
     1681 cggcgtcacg cgagcaccca ccccggcgcg cagttcaagg agccgatcac ggtcgcggac
     1741 gtcctggcgt cgaaaccggt ggcggctccg ctcaagctgc tcgactgctg cccggtgtcc
     1801 gacggcggcg ccgccttcgt cctgagccgc gagccgaccg gcgcttcccg ggtgcgcgtc
     1861 gccggtaccg gccaggcgca cacgcaccag cacgtcacgg ccgcgctcag cttgacgcag
     1921 ttcggcgcgg gcgcttccgc gcgacgcgcg atgcaggctg cgcacgtcgg cctggacgag
     1981 gtgaagtacg ccgcgatcta cgacagcttc accatcacgc tcacgatcct gctcgaggag
     2041 atcggcctgg ccccgcgcgg ccaggcgggt cggctggcgc gcgagggccg gttctcacgg
     2101 gacggacgct ttcccctcaa cacgcacggc gggttgttgt cctatgggca ttcggggtgc
     2161 gccggggcga tggcgcacct ggtggaggcg cagctgcaga tgaccaggcg cgccggcgcg
     2221 cggcaggtgc gcgacgcgtc ggtggccctg ttgcacggag acggcggggt gctgtcgaca
     2281 catgtgagcc tggtgctggc gaacccgtga tgcgggagct ggacgcgagg cgagaatgat
     2341 ccccgattgg acccaaggcg gcgagggcat cgcttaccag gcgtgcgtcg gttgcggcgc
     2401 cgtctggtac ttccgccgca ccttctgtcc gcgctgcggc cgtgcgtccc ccgaagatcg
     2461 cgcggccggt ggcgccggca cggttcacgc ggtgaccctg gtggcgcgcg cgccgagcga
     2521 ggagctgcgc gcgcatgcgc cctacgccat cgtgctggtc gacaccgacg aggggtttcg
     2581 gatgatggcc cacggcgacg tggatctgcg catcggcgac cgcgtgcaat gccgcttcat
     2641 ctccctggcg ggacgcatcg tgccgcgcta cgacaagcgc tagcggcgca atgggagcga
     2701 gaacgccttc tcgtccccgg attgcgcttc gctccatccg ggctacgcta ctgcctcacg
     2761 aatcacgaat cacgaatccc gacccatgac cgactcccgc cgcgccgcgc tgcgcaccgt
     2821 cctcgatccg cgctcgatcg ccgtcatcgg cgcttccgag aaccccaaca agatcggcgg
     2881 ccggccgctg ctctacttgc ggaagttcgg cttccggggc aaggtctatc cgatcaatcc
     2941 caaccgcaag gaggtgcagg gcttcaaggc ctatcccggc ctggcggcgt tgccggagac
     3001 gcccgaggtc gccatcgtag tcgtgcccga cgacctcgcc gtgcaggccg tggacgactg
     3061 tgccgcgcgc ggcgtgaaga ccgccatcgt gatggcgtcg ggcttcggcg agaccagcga
     3121 cccggcggcg caggacaagg aacggcgcat ggccgagcgc ggccgcgccg ccggcatgcg
     3181 catcgtgggc cccaacacgc agggcctggc caacttcgcc agcggcgccg tgctgagctt
     3241 ctccaccatg ttcctggaaa ccgaggcgcg cgacggcccg gtgggcgtgg tcagccagag
     3301 cggcgcgatg agcgtggtgc cctacgggct gctgcgcagc cggggcgtgg gcgtgcgcta
     3361 cgcctgcgcc accggcaacg actgcgacgt gagcgtgggc gagctcgctt ccgtggtggc
     3421 cgaggatgcg cagttgaagt tgctgctgct ctacctcgag gcgatccgcg atccggcgcc
     3481 gttcgtcgag ctggcgcgga tcgcccgcgc gcgcgggctg ccgatcgtcg cgctgaagtc
     3541 cgggcgcact cccgccgggc agcgcgccgc ccagtcgcac accggcgcgc tcgccaatga
     3601 ggaccgcgtg gtggaagctt tcctcgagcg ccacggcatc tggcgcgcgc gcgacgtcaa
     3661 cgagctggtg gacggcgccg agctgtacct caagggctgg aagccgaagg gccggcgcct
     3721 ggtcgcgatc agcaactcgg gcgcgagctg cgtgatggcg gccgacgcgg ccaccgaggc
     3781 cgggctcgcc acgcctgcgc tgagcgacga gacgcgcgcc gggctgtccc gaatcctgcc
     3841 gggcttcgcc accaccacga acccggtaga catcaccgcc gccctgctca gcaacagcag
     3901 cctgttcggc caaatcctgc ccgtcatcgc cagggacccg gcggccgatg cgttcttcat
     3961 cgccatcccg gtggcggggc agggctacga cgtggacacc ttcgccagcg atgccgccgt
     4021 gttcgcgcgg cgcaccggca agccggtggt gaccgccatt ccgcaggcga gcatcgcggc
     4081 caagttcaag gcgcaggggc tgccggtgtt cggcaccgac gccgaggcgg tgcgcgcgct
     4141 gggacgatat ctggcccacg tggagctgat gcagcgcatc gcggccgaac cttcgcgtgc
     4201 cgccccgatg cgccggcccg cgccgcccaa ttggcacgcg ctgaacgaag cggacagcct
     4261 ggagcgggtg cgccgtcacg gcgtgccggt ggtcgagcac cgcgtatgcc gcacaccgga
     4321 cgaggcgcag gccgctctgg ccgagctggg cggcccggtg gcggtgaagg gctgctcgcg
     4381 ggacgtgccg cacaagtccg aatgggggtt ggtgaagctg ggcctcacca ccgccgacca
     4441 ggtgcgcagc gcctacagcg acttcgacaa gacgctgcgc atcgccggcg ggcgcttcga
     4501 cggcctgatc gtggccaggc tggtcaaggg cctgcgcgag ctgatgatcg gcgcgcacct
     4561 cgacccggtg ttcggcccgg tggtcgtggt cggcgacggc ggcaagtacg tcgaagccat
     4621 gcccgacgtg caggtgctgc tggcgccgtt caccccggac gacgtgttcc gcgccctcca
     4681 gcgcctgcgc atcgcgccgc tcctggcggg ggtgcgcggc gaccccccgc tcgacgtgga
     4741 cgagttctgc aacgccgcgg ccagagtggg cgccatgatg gctgccgccg acagcgatgt
     4801 cgtcagcctc gacctgaacc cggttatcgt gcgggcgcag cgcgagggtt gcgaagcggt
     4861 ggatgcgctg gtggtggtcg cctcgccgcc ggcgtaggat gcgcggcgtg cactgcctgc
     4921 gctgaaatcc gaccgcgatg ccggcgtacg ctcacaccgt cgggtccctc acctatcgct
     4981 tcgccgacct gaagactttg atggcgaagg cgacgccggc gcgcaccggc gatgggctgg
     5041 cggggatcgc cgcggcgagc gccgaggagc gggtggccgc gcagatggcg ctcgccgacc
     5101 tgccgctcac cgcctttctc gccgagcact tggtgcccta cgaggaggac gaagtcacgc
     5161 gcgccatcct ggaccatcac gacgtccggg ccttcgcgcc ggtggctcac ctcaccgtgg
     5221 gcgagctgcg caactggctg ctgtcctacg aagcggacga agcggcgctg gccgctctgg
     5281 cgcccggcct cacgcccgag atggtggcgg cggtctgcaa gctgatgcgc atccaggacc
     5341 tggtgctcgc ggccgccaag tgccgggtgg tgacccgctt ccgcaacacc cttgggctgc
     5401 gcgggcgcct gtccagccgg ttgcagccga atcacccgac cgacgacccc aagggcatcg
     5461 ccgccagcat cgtcgacggg ctgctctacg gctgcgggga tgcggtgatc ggcatcaacc
     5521 ccgccaccga caatctcgag caggcgtgcg cgctgttagc gctgctggac gacatcatcg
     5581 ccggacaccg gatccctgcg cagtcgtgcg tgctcaccca cgtcacgacc acgctgcgcg
     5641 cgatcgaacg cggcgcgccg gtggacctgg tgttccagtc gatcggcggc accgaggcca
     5701 ccaaccgcag cttcggggtc agcgccgcgc tgctggacga ggcgaaccgg gcggcgcagg
     5761 ggctggcgcg cggcggcccg ggcgcgaacg tcatgtactt cgagaccggt cagggcagcg
     5821 cgctctcggc caacgcccac cacggcgtcg atcagcagac cctggaggcg cgcgcctacg
     5881 ggctggcgcg cctgtaccgg ccgctgctgg tgaacagcgt ggtgggtttc atcggcccgg
     5941 aatacctcga cggccgcgag atcgtgcgcg ccggcgtgga ggaccatttc tgcggcaagc
     6001 tgctcggcct gccgatgggc gtggacgtgt gctacacgaa ccatgccgat gcggaccagg
     6061 acgatatgga cacgctgctc acgctgctcg gcgcggccgg cgtcaattac atcatgggcg
     6121 tgccgggggc cgacgacatc atgctcaact accagagcac ttcgtaccac gacgcgctct
     6181 acctgcgcca ggccctgggg ctcagacccg cgccggagtt cgaagactgg ctgatgcgca
     6241 tgggcgtgtt cgacacgccc acgcggctgc ggccggccga ccctcgacat ccgctgctgg
     6301 ggctggtcgg cgggcgcggc gccgagtagc cgcaccgcgc cgcacgcgat gccccgatca
     6361 ccgaaaaccg cgctcggccg tcccgatccg tggcaggcgc tcaaccggtt caccgccgcc
     6421 cgtatcgggt tggggcgcgc gggtgggagc ctccccacca gacctctgct cgagttccag
     6481 ctgtgccatg cgcgtgcgcg cgacgccgtg cagcgcgatc tggacaccgt cgcgcaagag
     6541 cagcgcctgc gcgaatgcgg attcgaagtg ttgcagctgg cgagcgcggc ggccgatcgg
     6601 cacgccttca tccggcgccc ggatcagggc cgcatcctgc acccggactc gagagcgctg
     6661 ctcgaacggc gtcctccccg tgccgagccc tacgacgcgg tgttcgtcct ggccgacggg
     6721 ctctccgcgc tcgcggtgga gcgtcatgca accccgctgc tggagcggat cgcgccgcgc
     6781 ctgatcgacg ccggatggca tctcgctccc gtgtgcgtgg tgcggcaggg gcgcgtagcg
     6841 gtggccgacg agatcggcac cctgctgccg gcctgcatga gcgtcatgct gatcggcgag
     6901 cggcccgggc ttacctctcc cgacagcctg ggcatctacc tcacctggga cccgctgccg
     6961 ggccgcagca acgccgagcg caactgcatc tccaacgtgc gtcccgaggg cctggactat
     7021 gcgcacgccg cgcacaagct gttctacctg atgagcgaat cgcggcggcg ccggctctcg
     7081 ggcgtcgatc tgaaggagga cgctccggcg ctccccgccg ccggctccgg agcgctgccg
     7141 ggtgcgggga gcggcccgcg atgacgcggg ctgcatcccg gctgcatccg gcgagcacgc
     7201 cgtgatcggc ctcgcccgtg ctcgcgccca gcgctcgcac gcggtgtcgc tgaccggcga
     7261 acggccgggc gcgcacgggg gaaaatcgac ccgtcccccc atccggcggg gggttcgcac
     7321 acataacgat tgactcgcgt gacactcttg tgcatcctct gctcgccgca cggcaaatct
     7381 ctctgtgacg agacatccaa cttggagaca tgacgatggc acaagccaag aaggcgagga
     7441 agaagaccac tgcgagaaag cccgccagga aggccccggc gaagaagcct gccaagagga
     7501 aggcggcgcc gaagaaagcc ccgactcgga aagccgcccg gaaacccgcc aagaaagccg
     7561 ctccgaagcg caagcgcacc ccgaacgcgg ccttcatgaa ggcgatgacc cccagcgggg
     7621 atctggccgc cgtggttggc gaccgggcca tgccgcgcac cgaagtcacc aagaagatct
     7681 gggactacat caagcgcaac aagctgcagg acagcgccaa ccggcgcatg atcaacgccg
     7741 acgacaagct caggccggtc ttcggcggca agcgccaggt gtcgatgttc gagatgacca
     7801 agctggtcaa caagcacctg aagtaaaggt ttctaccctt caggtaagag cttctac
//
LOCUS       DASPGE010000416         5192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_464583, whole genome shotgun sequence.
ACCESSION   DASPGE010000416 DASPGE010000000
VERSION     DASPGE010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464583"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VD791_13720"
     CDS             <1..1065
                     /locus_tag="VD791_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV09087.1"
                     /translation="TKELGGYRVLCEKIGAQSELEIVVDSVDGFKQELARVAELVREA
                     GLKLSAVAVCPVGDLKSVLPGGARPPAPPLEQLYQAARDAFPGVKLGGGMFSFFTELN
                     RKRPPAALLDFVHNGTCPIVHAADDRSVMETHEALPYQITTARSFIGKTPYRVGPSSI
                     GCRDNPHGKTFTPNPDNQRICLVKMDPRQRGLFGAAWALGYLSSLAPTGVETISLGAP
                     TGPLGIIYRKTDYAQPYYDGLGRDAVYPAFHVVAGLTRAAGAKLVSATSSDDASVRCL
                     AYRAKGATLLWLANMTAGDQAVTIAHEGANLFGVVLDESTFGKATADPRAFQRSWTAL
                     SAAKLKLTAYAVAALCIDDR"
     gene            1131..2264
                     /locus_tag="VD791_13725"
     CDS             1131..2264
                     /locus_tag="VD791_13725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HXV09088.1"
                     /translation="MNVACIEDLRRIARRRLPRAIFEFIDGGAQDEMTLRRNCEDFQR
                     ILLVPRALTDVSQRDQSVTLLGQTYSSPLILAPTGTPGVVWPNGAIVAALAAEAAGVG
                     YCLSTMSTSSIEEIARASKRPFWFQLYVMRDRELGKSMMQRAKAAGCCALVITVDLQM
                     QGQRDRDVRNGLTIPPQLRFASALDFALHPGWVWRLLTGPKITLANFAGSGKGGDLFT
                     IAEFVNAQFDPSVTWKDIDWVRSVWDGPLALKGILDAEDAKLAVEHGVEAVIVSNHGG
                     RQLDGVPSAISALPAVVDAVGDRAEVILDGGVRRGNDVLKALAIGAKACMIGRPFLYG
                     LAANGGAGVRKALELLRAEIDVNLALLGRAGVRDLDRSAVSWT"
     gene            2343..3608
                     /locus_tag="VD791_13730"
     CDS             2343..3608
                     /locus_tag="VD791_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV09089.1"
                     /translation="MASAVGSALEWYDFFIYGTAAALVFGQLFFPKSDPTTGTLLAFA
                     TFGVAFLARPVGGMVFGHLGDKLGRKPVLVMTLMLVGGGTFLIGLLPTYDSIGAWAPV
                     LLVLMRLLQGFGAGAEYGGAVILAVEHAPPGKRGLFGSVAPLGVTVGLLLANGVFAVV
                     GTLPDEAFLSWGWRVPFLLSIVLILVGFYIRARVSETPAFTEIAARNKLARSPVKEAV
                     VKHPREFLVVIGARLAENGLGFLYPVFALNYMTQQLGLSKTMVLQGNMLAYTVQLATI
                     PLFSMLSDKIGRRPVYLGGALFSAAFAVPFFMLVNTQSPPLIWLALVLGISVGVGAMF
                     GPQAAYFAELFGTRTRYSGFAFARELGSILAGGPAPFVAKVLVIWAAGQPWGVAGYMM
                     AMALITALAVYCGPETYKADIHAEGADKA"
     gene            complement(3624..4097)
                     /locus_tag="VD791_13735"
     CDS             complement(3624..4097)
                     /locus_tag="VD791_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV09090.1"
                     /translation="MSNDPYTPPSAPVRDRQPEAPGSTWKAVTFGVLSDIVGTFLAGI
                     VLFALLGSMLVSQGASPEELDRALMESDAYMLLAGVTGLGFTVLGGYVAARVANQREY
                     YHALLTGLVVLVFGELMIGISGQEYPLAYRIIGDLLAVPAALLGGHLRKTAKLKG"
     gene            4261..4707
                     /locus_tag="VD791_13740"
     CDS             4261..4707
                     /locus_tag="VD791_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV09091.1"
                     /translation="MNRWMGLAMLTAAALAPGCATETEVAGTTQVGKVEQVFVEAYPG
                     LFVDRALATVVGDRQAWASVTFARPLSDGRTTAVAMLERDSGVDPGDLVQMRFAHPGA
                     FDPDTLPGHNQVTALVAKHDSRAALSFGRGQAALSPDKLTAADPLP"
     gene            4764..5147
                     /locus_tag="VD791_13745"
     CDS             4764..5147
                     /locus_tag="VD791_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV09092.1"
                     /translation="MKSGITLIALLFAGLLVHGYARTANVSRGPHLGIVEAVFEEVRP
                     GVFTPRSHGAKRSQAPAWAHVRFPQPLEDGRTTAVAAVPARFDLQAGDLIHMRFGEPV
                     SAASDAPADDTVTALVASNRSRRMR"
BASE COUNT          768 a         1775 c         1806 g          843 t
ORIGIN      
        1 acgaaggagc tgggaggcta tcgcgtcctg tgcgagaaga tcggcgcgca gtccgagctg
       61 gagatcgtgg tcgacagcgt cgatgggttc aagcaggagc tggcgcgcgt ggccgagctg
      121 gtgcgcgaag cgggcctgaa gctctcggcc gtggcggtgt gcccggtggg cgatctcaag
      181 tcggtgctgc ccggcggtgc gcggcccccc gccccgccgc tggagcagct gtaccaggcc
      241 gcccgcgatg cgttccccgg cgtcaagctg ggcgggggca tgttctcctt cttcaccgaa
      301 ctcaaccgca agcgtccgcc ggccgcgctg ctggacttcg tgcacaacgg cacctgcccg
      361 atcgtgcatg cagccgacga ccgttcggtg atggagacgc acgaggcctt gccctaccag
      421 atcaccaccg cgcgcagctt catcggcaag acgccctacc gcgtcggccc cagctcgatc
      481 ggctgccgcg acaacccgca cggcaagacc ttcaccccca accccgacaa tcagcgcatc
      541 tgcctggtga agatggatcc gcgccagcgc ggcctgttcg gtgcggcgtg ggctctgggc
      601 tatctgtcga gcctcgcgcc caccggcgtg gagacgatca gcctgggcgc gccgaccggt
      661 ccgctgggga tcatctatcg caagacggat tacgcgcaac cctattacga cggactcggg
      721 cgcgacgcgg tgtaccccgc gtttcacgtc gtcgccggac tcacgcgcgc ggcgggcgcc
      781 aagctggtgt ccgcgacctc ttccgacgac gccagcgtgc gctgcctggc gtaccgggcg
      841 aagggcgcca ccttgctgtg gctggcgaac atgaccgcgg gcgaccaggc cgtgaccatc
      901 gcgcacgaag gcgcgaatct gttcggggtg gtactggacg agtcgacctt cggcaaggcg
      961 acggcggacc cgcgcgcctt ccagaggagc tggacggcgc tgagcgctgc caagctgaag
     1021 ctcacggcct acgccgtcgc ggcgctttgc atcgacgacc ggtgaagccg tgattcgtga
     1081 atcgtgattc gtgacaacga agcaccgccc gccatcttcg ccgcgcctga atgaacgtcg
     1141 cctgcatcga agacctacgc cgcatcgccc ggcggcggct gccgcgcgcc atcttcgagt
     1201 tcatcgacgg cggcgcgcag gacgaaatga cgctgcggcg caactgcgag gacttccagc
     1261 gcatcctgct cgttccgcgc gcgctcaccg acgtctcgca gcgcgatcag tcggtcacgc
     1321 tgctcgggca gacttacagc tcgcccctga tcctggcccc taccgggacg ccgggcgtgg
     1381 tgtggccgaa cggggcgatc gtcgccgcgc ttgccgcgga agccgccggc gtcgggtatt
     1441 gcctcagcac catgtcgacc tcgagcatcg aggagatcgc gcgggcgtcg aagcggccgt
     1501 tctggtttca gctctacgtg atgcgcgacc gcgagctggg caagagcatg atgcagcgtg
     1561 ccaaggccgc cggctgctgc gcgctggtga tcacggtgga cctgcagatg cagggccagc
     1621 gcgatcgcga cgtgcgcaac ggcctcacca taccgccgca gctgcgcttt gccagcgcct
     1681 tggacttcgc cttgcacccg ggctgggtgt ggcgccttct gaccgggccg aagatcaccc
     1741 tggcgaactt cgcgggcagc ggcaagggcg gcgatctgtt caccatcgcc gagttcgtga
     1801 acgcgcagtt cgacccctcg gtcacctgga aagacatcga ctgggtgcgc tcggtgtggg
     1861 acgggccgct cgccctgaag ggcattctcg acgccgagga cgcgaagctc gcggtcgagc
     1921 acggcgtcga agcggtgatc gtgtcgaatc acggcggccg ccagctcgac ggcgtgccct
     1981 cggcgatctc cgcgctgccg gcggtggtgg atgcggtagg cgaccgcgcc gaggtgatcc
     2041 tcgacggcgg cgtgcggcgc ggcaacgatg tgctcaaggc gctcgcgatc ggcgccaagg
     2101 cgtgcatgat cggcaggccg tttctctacg gtctcgcggc gaacggcggc gccggggtgc
     2161 gcaaggcgct cgagctgctg cgcgccgaga tcgacgtcaa cctggcgctg ctcggccgcg
     2221 ccggcgtgag ggacctggat cgctccgcag taagctggac ctgatgcgcc ctgaaggcgc
     2281 gacttaagaa accgctctct cctcgagtac ccgacatgga tacagcacac cgcagagtac
     2341 tgatggcttc cgccgtaggc tcggcgctgg agtggtacga cttcttcatc tacggcaccg
     2401 ctgccgcact ggtgttcggc cagctgttct tcccgaagag cgatcccacc accggcaccc
     2461 tgctcgcttt cgccaccttc ggggtggcgt tcctggcgcg tccggtcggc ggcatggtat
     2521 tcgggcacct gggcgacaag ctggggcgca agccggtgct ggtgatgacg ctgatgctgg
     2581 tcggcggcgg caccttcctg atcgggctgc tgcccactta cgattccatc ggcgcgtggg
     2641 cgccggtgct gttggtcctg atgcgcttgc tgcaaggctt cggcgccggc gccgagtacg
     2701 gcggcgcggt gattctcgct gtggagcacg cgccacccgg caagcgcggc ctgttcggca
     2761 gcgtcgcacc gctcggcgtg accgtcggcc tgctgctcgc caacggcgtg ttcgccgtgg
     2821 tcggcacact acccgacgag gctttcctga gctggggctg gcgcgtgccg ttcctgctga
     2881 gcatcgtgct gatcctggtg ggcttctaca tccgcgcccg ggtgagcgag acgccggcgt
     2941 tcaccgagat cgcggcgcgc aacaagctgg cgcgctcgcc ggtcaaggag gcggtcgtca
     3001 agcatccgcg cgagttcctg gtggtgatcg gtgcgcgcct ggcggagaac gggctcggct
     3061 tcctgtaccc ggtgttcgcg ctgaactaca tgacgcagca gctgggtctg tccaagacca
     3121 tggtgctcca gggcaacatg ctggcttaca cggttcagct cgccaccatt ccgctgttct
     3181 cgatgctgtc ggacaagatc ggccggcgcc cggtatacct gggcggtgcg ctgttctcgg
     3241 cggcgttcgc ggtcccgttc ttcatgctgg tcaacacgca gagcccgccg ctgatctggc
     3301 tggcgctggt gctcgggata tcggtgggag tgggcgccat gttcgggccg caggcggcct
     3361 acttcgccga gctgttcggc acccgtacgc gctacagcgg cttcgccttc gcgcgcgagc
     3421 tgggttcgat cctcgcgggc ggccccgcac ctttcgtcgc caaggtgctg gtgatctggg
     3481 ccgccggcca gccctggggc gtggccggtt acatgatggc gatggcgttg atcaccgcgc
     3541 tggccgtgta ctgcgggccg gaaacctaca aggccgacat ccacgccgag ggcgcagaca
     3601 aggcgtagcg actcggcgcg acctcagcct ttgagcttcg cggtcttgcg caggtggccg
     3661 ccgagcaggg cggcgggaac cgcgagcaga tctccgatga tgcggtaggc gagcggatac
     3721 tcctggcccg atatgccgat catcagctcg ccgaacacca gcaccaccag gcccgtgagc
     3781 agcgcgtggt aatactcgcg ctggttggcc acccgcgccg cgacgtagcc acccagcacg
     3841 gtaaagccca gcccggtcac cccggccagc agcatgtatg cgtccgactc catcagggca
     3901 cggtccagct cctcgggact ggcgccctgc gacaccagca tggatccgag cagggcgaac
     3961 agcacgatgc cggcgaggaa cgtcccgacg atgtcggaca gcacgccgaa ggtcaccgcc
     4021 ttccaggttg aacccggtgc ttccggctgg cgatcgcgca ccggggcgga aggcggggtg
     4081 taggggtcgt tgctcatcgg ttgtttcctg caggcgactg tcctcgcgcg ggggaagcaa
     4141 ttatccagcc gatgcgccgc ggctgcacgc gggcggccct gctcaagttc accacgggcg
     4201 cgccgttacc tcgtcagagc gggcccccca gagggacccg ggcgagcgag gagtcgggcg
     4261 atgaacagat ggatggggct ggcgatgctg accgcagcgg ccctggcgcc gggatgcgcc
     4321 acggaaaccg aggtcgccgg caccacgcag gtcggcaagg tcgagcaggt tttcgtggaa
     4381 gcctaccccg ggttgttcgt cgatcgcgcc ctggcgaccg tcgtcggcga tcggcaggca
     4441 tgggcgtcgg tgaccttcgc ccgccccctg agcgatggcc gcaccaccgc cgtggccatg
     4501 ctcgagcgcg attccggcgt cgacccgggc gacctggtgc agatgcgctt cgcccatccc
     4561 ggggccttcg accccgacac cctccccggg cacaaccagg tgaccgcgct ggtcgccaag
     4621 cacgacagcc gggcggccct gtccttcggc cgcggccagg cggcgctgtc gccggacaag
     4681 ctcaccgcgg ccgacccgct gccctgatcg cccgtgccct gatcgccccg ctgccctgat
     4741 aacctagcgc ccgcgcatcc gccatgaaga gcggtatcac gctgatcgcg ctgttgttcg
     4801 ccggattgtt agtgcatggt tacgcgcgca ccgcgaacgt gagccggggg ccgcatctgg
     4861 gcatcgtcga ggcggtattc gaggaggttc gcccgggcgt cttcacgcct cggagccatg
     4921 gggcgaagcg cagccaggcg ccggcctggg cgcacgtgcg atttccccag ccgctggagg
     4981 atggccgcac caccgcggtg gcggcggttc cggcgcggtt cgacctgcaa gccggcgacc
     5041 tgatccacat gcggttcggc gagccggtgt ccgcggcaag cgacgctccg gccgacgata
     5101 cggtcaccgc cctggtggcc agcaaccgca gccggcggat gcgctgaacg cgcatcgaag
     5161 gcccgcgtag ccctgctacg cgtggaagta cg
//
LOCUS       DASPGE010000417         3033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_580998, whole genome shotgun sequence.
ACCESSION   DASPGE010000417 DASPGE010000000
VERSION     DASPGE010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580998"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..102)
                     /locus_tag="VD791_13750"
     CDS             complement(<1..102)
                     /locus_tag="VD791_13750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV09093.1"
                     /translation="MKTILIMAGGTGGHVFPGLSVADEMRSAGWRVVW"
     gene            complement(278..1444)
                     /gene="ftsW"
                     /locus_tag="VD791_13755"
     CDS             complement(278..1444)
                     /gene="ftsW"
                     /locus_tag="VD791_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HXV09094.1"
                     /translation="MIALSTPARPAAALQDFDPGLIWVGVLLIGVGLVMVYSASIAIA
                     EGARYTDGNAAYFLLRHALALAVSAAVAFCVFQLPMRLWQRAAPYLFLAGALLLVLVL
                     IPGIGREVNGSRRWLPLGVMTLQPSELMKLFAVLYTADYAVRKAAWMHSLKKGFLPML
                     AVMLFTGALLLREPDFGAFAVIACISMGVLFLGGMNWKLFVGLFVLLAIGFVVVILTS
                     PYRMQRILGFMDPWADPFGKGYQLSHALIAFGRGEWFGVGLGASVEKLFYLPEAHTDF
                     LLAVIGEELGFFGVTAVILLFVWLTARAFSIGAQAAKLERYFPALVAQGIGIWLGAQA
                     LISMGVNMGVLPTKGLTLPFLSFGGSGLLANCCALALLLRIDFESRQLMRGRSA"
     gene            complement(1441..2826)
                     /gene="murD"
                     /locus_tag="VD791_13760"
     CDS             complement(1441..2826)
                     /gene="murD"
                     /locus_tag="VD791_13760"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HXV09095.1"
                     /translation="MELAGKQALVLGLGVSGLSMARWLASRGAHVRVADTRATPPCAP
                     QLVRDLPAVRVAIGPWREDIFAGIDLIALSPGIPAGEPSIAAAAARGVPVVGDIELFA
                     QALPALGPVKVLAITGSNGKSTVTEMTGAMCRAAGLATVVAGNIGLPVLDVLLDIERR
                     PQEPRPEAFVLELSSFQLETTSGLDAQVAVCLNLSEDHLDRYDNMRDYAQAKARIFRG
                     SGVQVLNRQDAFSRAMALPGRRVLTFGLDAPASERDWGLLEGDGATWLAQGDRRLMSV
                     CQLRVTGLHNAANALAALALGRAVDLPYEPLLRALAQFEGLPHRVQKIAQLGGVVFFD
                     DSKGTNVGATVAALNGMRQRVVLIAGGDGKGQDFGPLQAAVAGRARAVVLIGRDRERI
                     AETLTGAGVPLLRAADMEEAVRLAFSQARDGDAVLLSPACASFDMFRNYEHRAQAFAE
                     AVRKLDHSIPA"
     gene            complement(2826..>3033)
                     /locus_tag="VD791_13765"
     CDS             complement(2826..>3033)
                     /locus_tag="VD791_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HXV09096.1"
                     /translation="MGGVFVVETLSVMLQVASFKLTGKRIFRMAPLHHHYELKGWKEN
                     QVVVRFWIITMMLVLVGLSTLKLR"
BASE COUNT          547 a         1092 c          995 g          399 t
ORIGIN      
        1 ccacaccacg cgccagccgg cgctgcgcat ctcgtcggca actgacaatc cgggaaaaac
       61 gtggcctcca gtccctccgg ccatgatcag aatggtcttc attgccacca ccgtctatca
      121 gaccgctggc cacgttttcg gtggaggcta accggcgcgg cgcgccggtt aagcgcgcgc
      181 ccgacggatt caaacagcag gggccgcgcg cggccggaac caaatacatg accgccagtt
      241 ccgccagcca tgataagaat ggtgtggctg gcgacgctca tgcgctgcgc ccccgcatca
      301 attgtctgct ctcgaagtcg atcctgagca gcagcgccag cgcgcagcag ttggcgagca
      361 ggccactgcc gccgaagctc aggaacggca gggtgagtcc cttggtgggc agcacgccca
      421 tgttcacgcc catgctgatc agggcctgcg cgccgagcca gatgccgatg ccctgcgcca
      481 ccagcgcggg gaagtagcgc tccagcttgg cggcctgggc gccgatcgag aaggcgcgtg
      541 cagtgagcca gacgaacagc aggatcaccg ccgtgacacc gaagaagccc agctcctctc
      601 cgatcaccgc cagcaggaaa tcggtgtgcg cttccggcag atagaacagc ttctcgacgc
      661 tcgcccccag gcccacgccg aaccactcgc cgcgcccgaa cgcgatcagc gcatgcgaca
      721 gctgatagcc cttgccgaag gggtcggccc acggatccat gaatccgaga atccgctgca
      781 tgcgataggg tgaagtgagg atgacgacca cgaagccgat cgccagcagc acgaacaagc
      841 cgacgaacag cttccagttc atgccgccca ggaacagcac gcccatggag atgcacgcga
      901 tcaccgcgaa cgcgccgaag tccggctcgc gcagcagcag tgcgccggtg aacagcatca
      961 ccgcaagcat cggcagaaag cccttcttca gactgtgcat ccacgccgcc ttgcgcacgg
     1021 cgtagtcggc cgtgtacagc accgcgaaca gcttcatcag ctccgagggc tgcagggtca
     1081 tcactccgag cggcagccag cgccggctgc cgttcacctc gcgcccgatg cccgggatca
     1141 gcaccaacac cagcagcagc gcgccggcca ggaacaggta cggcgcggcg cgctgccaca
     1201 gccgcatcgg cagctggaac acgcagaatg cgaccgccgc gctcacggcg agcgcaagcg
     1261 catggcggag caggaaatag gcggcgttgc cgtcggtgta gcgcgcgcct tcggcgatgg
     1321 cgatggaggc cgagtacacc atcaccagac ccacgccgat caagagcacg ccgacccaga
     1381 tcagaccggg atcgaagtcc tgcagggcgg cggcggggcg ggcgggcgtg gacagcgcga
     1441 tcatgcgggg atggaatgat ccagcttgcg caccgcctcg gcgaacgcct gcgcgcggtg
     1501 ctcgtagttg cggaacatgt cgaagctggc gcaggcgggg gacagcagta cggcgtcccc
     1561 gtcgcgcgcc tgggagaagg ccaggcgcac cgcctcctcc atgtcggccg cgcgcaacag
     1621 cggcacaccg gcgccggtca aggtctccgc gatgcgctcg cggtcgcgtc caatcagcac
     1681 cacggcccgc gcgcggccgg ccaccgccgc ctgcagcggg ccgaagtcct gccccttgcc
     1741 gtcgccgccc gcgatcagca ccacccgctg gcgcatgccg ttgagcgccg cgacggtggc
     1801 gccgacgttg gtgcccttgg agtcgtcgaa gaagaccacc ccgccgagct gggcgatctt
     1861 ctgcacgcga tgcggcagcc cctcgaactg cgccagcgca cgcagcagcg gctcgtaggg
     1921 caggtccacg gcgcggccga gcgcgagcgc ggccagggcg ttggccgcat tgtgcaggcc
     1981 ggtcacgcgc agctgacata ccgacatcag cctgcggtcg ccctgcgcca gccaggttgc
     2041 gccgtcgccc tccagcaaac cccagtcgcg ctcggaagcc ggcgcatcca ggccgaaggt
     2101 cagcacgcgg cgcccgggca gcgccatcgc gcgcgagaac gcgtcctgcc ggttgagcac
     2161 ctgcacgccg ctgccgcgga agatgcgcgc tttggcctgc gcgtagtcgc gcatgttgtc
     2221 gtagcggtcc aggtggtctt cgctcaggtt gaggcagacg gcgacctggg cgtccaggcc
     2281 ggaggtggtc tccagctgga agctggacag ctccagcacg aaggcctccg ggcgcggctc
     2341 ctgcggccgg cgctcgatgt cgagcagcac gtcgaggacc ggcagcccga tgttgccggc
     2401 caccaccgtg gcgagccccg ccgcgcggca catcgcgccg gtcatctcgg tcaccgtgct
     2461 cttgccgttg gacccggtga tcgccagcac cttcaccggg cccagcgccg gcaacgcctg
     2521 cgcgaacagt tcgatgtccc cgaccacggg cacgccgcgc gcggcggccg cggcgatcga
     2581 cggctcgccg gccgggatgc cgggactcaa tgcgatgagg tcgatgccgg cgaagatgtc
     2641 ctcgcgccat ggcccgatgg cgacccgcac cgcgggcagg tcgcgaacga gctgcggcgc
     2701 gcacggcggc gtggcgcgcg tgtccgccac gcgcacgtgc gcgccgcggc tagccagcca
     2761 gcgcgccatc gacaggccgc tcactcccag cccgagaacc agcgcttgct ttccggcgag
     2821 ctccatcagc gcagcttcaa agtcgacagc ccgaccagca ccaacatcat cgtgatgatc
     2881 caaaaccgca cgaccacctg gttctctttc caacctttca gctcgtagtg gtgatgcagc
     2941 ggcgccatgc ggaagatgcg ttttccggtg agcttgaagg aggccacctg cagcatcacc
     3001 gagagcgtct cgaccacgaa caccccgccc atg
//
LOCUS       DASPGE010000418         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_465037, whole genome shotgun sequence.
ACCESSION   DASPGE010000418 DASPGE010000000
VERSION     DASPGE010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465037"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VD791_13770"
     CDS             complement(<1..252)
                     /locus_tag="VD791_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HXV09097.1"
                     /translation="MRAGRALIGDEMGLGKTIQAIAAAEILARHFGVSKVLVICPTSL
                     KYQWQSEIARFSGRNSHVIAGGRAQRQKDYARDDFCKITN"
     gene            357..1682
                     /locus_tag="VD791_13775"
     CDS             357..1682
                     /locus_tag="VD791_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HXV09098.1"
                     /translation="MSWAAEEFETLDLGDRRLNRRAVLLAERLAAQPTASIPAACGGW
                     AETQAAYRLLADDDIEWADILAPHLACTQHRMAVHPVVLCIQDTTELDFNGQAIAGLG
                     PLSFEAQRGMYLHPTYAVSLEREPLGVLDAWMWAREPKGPDGTRGGISESVRWIEGYE
                     RIAEQAAALPATRLVYVADREADILALMVRAHTLGCPADWLVRAQHNRALPEGDKLWA
                     RVGAGEVLGEIRFTLPSRQGQKARPVRQQVRAARVTLRAGAGKTIEVSCVIAREIDAP
                     VGVTPVEWRLLSNREAATFEAASELIDWYRARWEIELFFHVLKNGCKVEALQLATSAR
                     LERALTLFMVIAWRIGRLMRLGRACPELEAELLFERDEWEAAFILNKRRPPKTPPPLN
                     EVLRLVATLGGFLGRKGDGEPGVKTIWLGLQRVMDFAAGLKYARGDHDE"
     gene            complement(1703..2040)
                     /locus_tag="VD791_13780"
                     /pseudo
     CDS             complement(1703..2040)
                     /locus_tag="VD791_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          314 a          680 c          709 g          337 t
ORIGIN      
        1 gttggtgatc ttgcagaaat cgtcccgcgc atagtccttc tgccgttgcg cccgcccgcc
       61 ggcgatgaca tgcgaattgc gcccggagaa gcgggcgatt tcgctctgcc actggtactt
      121 gagtgaggtc ggacagatca ccagcacctt cgacacgcca aaatgccggg ccagaatctc
      181 ggcggcggca atggcctgga tggtcttgcc gagtcccatc tcgtcaccga tcagcgccct
      241 gcccgcccgc acggcgaaca aggcgccttc ggcctgatac ggggtagagc catcccatta
      301 cacacaacat gaatgtcatc ccgtaacatc ctgatttttt ggatcggatg acacgcatga
      361 gctgggctgc cgaagagttt gagacgctcg acctgggcga ccggcggctg aaccggcggg
      421 cggtgctgct ggccgagcgc ctcgccgcgc agcccacggc gagcattccg gccgcctgcg
      481 gcggctgggc cgagacgcag gccgcgtacc ggctgctggc ggatgacgac atcgagtggg
      541 ccgacatcct ggcgccgcat ctggcgtgca cccagcaccg catggccgtg cacccggtgg
      601 tgctgtgcat ccaggacacg accgaactcg atttcaacgg ccaggccatc gcaggcctgg
      661 ggccgctgtc gttcgaagcg cagcgcggca tgtatctgca tccgacctat gcggtgtcgc
      721 tcgagcgcga accgctcggg gtgctcgacg cgtggatgtg ggcgcgcgaa ccgaaggggc
      781 cggacggcac gcgcggcggg atcagtgaga gcgtgcgctg gatcgaaggc tacgagcgca
      841 tcgccgagca ggcggccgcc ttgcccgcca cccggctggt gtatgtcgcc gaccgcgaag
      901 ccgacatcct ggcgctgatg gtgcgggcgc acacgctggg ctgcccggcc gactggctcg
      961 tgcgcgcgca gcacaaccgc gcgctgcccg agggcgacaa gctgtgggcg cgggtcggtg
     1021 caggcgaggt gctcggcgag atccgcttca cgctgccttc gcgccagggg caaaaggcgc
     1081 gcccggtccg ccagcaggtg cgcgctgcgc gcgtgacgct gcgcgcgggg gcgggcaaga
     1141 cgatcgaggt cagctgcgtg atcgcccgcg agatcgatgc gcccgtgggc gtcacccccg
     1201 tcgagtggcg gctgctcagc aaccgcgaag cggcgacgtt cgaagccgcg agcgaactga
     1261 tcgactggta tcgggcgcgc tgggagatcg agctgttctt ccacgtgctc aagaacggct
     1321 gcaaggtcga agcgctgcaa ttggcgacga gcgcgcgtct ggagcgggcg ctgacgctgt
     1381 tcatggtcat cgcctggaga atcggccggc tgatgcggct cgggcgcgcc tgcccggagc
     1441 tggaggccga gttgctgttc gagcgcgacg aatgggaggc ggccttcatc ctcaacaagc
     1501 gccgtccgcc gaagacgccg ccgccgctca atgaagtcct ccgcctggtg gccacactcg
     1561 gcggctttct cggccgcaag ggcgacggcg agcccggcgt gaagacgatc tggctcggcc
     1621 ttcagcgggt catggacttc gcggcggggc tcaagtacgc gcgcggcgat catgatgaat
     1681 gagttgtgtg taatgggatg agtttgacga cgtgcagatc gatcccggct tccttggccg
     1741 cttgcttcgc cgcctcgccc gtgtagccct ggtcggccca cgccaccttc accgattcac
     1801 cggtggcggc ttgcaccgcc tcgcacaact ccttgacctg cgcgcgctcc tgctcgttgg
     1861 cgggtgtgac ggtcagtgcg accaactgcc ccaaggtatc cacggccata tgcaccttgc
     1921 tgccccgacg tcgcttgtag ccatcgtagc cggcgcgcgg cccgctctcg caggtgctct
     1981 gcagcgtgcg tccgtccagg atcacggcac tgggctgccc cttgcgggcg tcgcccgcgc
//
LOCUS       DASPGE010000419         7101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1048611, whole genome shotgun sequence.
ACCESSION   DASPGE010000419 DASPGE010000000
VERSION     DASPGE010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7101
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048611"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1053)
                     /locus_tag="VD791_13785"
     CDS             complement(112..1053)
                     /locus_tag="VD791_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV09099.1"
                     /translation="MDTPLAELTAEAAVPAPAPRAAAWRRLLRNWSVRIGGALLVLLT
                     LVSVLAPWLGTVDPTLIDPVNRDLLPGARAEVVTLTGESFEHRFWMGSDSYGRDIYSR
                     VLYGGRVSLVVGIAVALLSLFFGLIVGLTAGFLRRLDAVVMRVMDGLMAIPGILLAIA
                     LVALWRPSVPTVILAITVPEIPRVVRLTRALVLSIREEPYVEAAVSVGTTTLRLLYRH
                     VLPNTVAPLIVQGTYICAFAILVEAILSFLGVGLSTDVPTWGNIMAEGRLQFTQYPHN
                     VLFAGVFLALTVLAVNILGDGLRDTLDPRFSKRGDRP"
     gene            complement(1053..1994)
                     /locus_tag="VD791_13790"
     CDS             complement(1053..1994)
                     /locus_tag="VD791_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV09100.1"
                     /translation="MIRFLLQRLLATIPVLLVVAVVVFLLLRLTPGDPAAVIAGDIAT
                     TEQIEHIRESLGLNRPLVVQFGIWFGNALQGDLGESFFFRKSVAELIVQRLEPTVALA
                     LCTIVLAVAVAVPLGVLAAWRHGGWLDRALMGFSTLGFSIPVFVLAYLLIWLVSLELG
                     WLPVQGYRRLAEGFGPFIRHLILPSLTLSVIYIALIARVTRAAVSEALTEDFVRTARA
                     KGLTERRVLIRHALANAAVPIVTVIGIGLALLIGGVVVTESVYAIPGLGRLTVDAVLA
                     RDFPTIQGVILLFSVAYVLINLAIDLAYLALDPRIRY"
     gene            complement(1998..3572)
                     /locus_tag="VD791_13795"
     CDS             complement(1998..3572)
                     /locus_tag="VD791_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV09101.1"
                     /translation="MKPWFRPAAVMLAALCAALPLHARSEKVVRVTPHSNLTILDPGW
                     TTAYITRNHGYMIYDTLYGMDARNQIRPQMVERHEVSKDRKTWTFTLREGLEFHDGKP
                     VTSADVVASLTRWAKRDSMGEMLLRYTQALEAVDARTFRLKLTQPYGLVLESLGKPSS
                     YVPFILPKRVADTPADRQIDDYTGSGPFVFSKDEWKPGERVVYRKNTRYRPRGEAASG
                     TAGGKVAKVDRVEWVIIKDPQTQANALAAGEIDIIEYPAHESYAAFKTDPNIRIVEPN
                     PLGYAAMLRFNHLQPPFDNPKVRRAAMAALNQPAFLRTQIGIPEMYRTCFSVYPCKTR
                     YETSKGMELIAKPDMKRAQALLKESGYDGTPIVLMQPADVAPAAKFPVVATQLLRQAG
                     FKVDMQAMDWQTLVSRRAKKDPPAKGGWNIFITFWASVDGLNPVAMNALNAGCDKAWF
                     GWPCDAELEKLRDAFARTADDAVRKSIAEQIQARAMEIVTHVPIGEFQIPLAARKSVT
                     GFVTGYFLVFWNIDKA"
     gene            complement(3663..4403)
                     /locus_tag="VD791_13800"
     CDS             complement(3663..4403)
                     /locus_tag="VD791_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXV09102.1"
                     /translation="MQQTDAMVEALKRTLKARAITYAQVAQALRLSEPSVKRLFATGS
                     FTLERFEQICELARTSIGELARAVDGGKDEVSQLTPEQERTIMADPKLLAVALCALSH
                     MTLEQMVQTYTLSKTECIGLLLKLDRIKFLELLPNNRIKLRVTRAFTWLPNGPIQQYF
                     KARAQREYFSSSFDRPGETMLLVNGMLSKASSEAVIARLRRIANEFADLHHADAHLAL
                     GERRPASLLLAIRPWELEDLRALRRRRG"
     gene            4562..4795
                     /locus_tag="VD791_13805"
     CDS             4562..4795
                     /locus_tag="VD791_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV09103.1"
                     /translation="MRTDTLSGAYATKLGRPAQPALRPASSLPLRPRPLPPARPTEPP
                     LPDSEALFIALRRSLASCRDTTQLRMLGRLTRP"
     gene            complement(4880..5539)
                     /locus_tag="VD791_13810"
     CDS             complement(4880..5539)
                     /locus_tag="VD791_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HXV09104.1"
                     /translation="MQEMDLASLRALSAAVLSDVMDSLGLGARAMKPFVRPLDESLVL
                     VGRARTGLYMPVYRVHEGENPYEVEIALVDDLKPGDAVVLACGGPTDRIAPWGELLST
                     ASRARGAAGCVTDGLVRDARQIREMGFPVFHGGIGPLDTKGRARMMERDTPVDCGGVH
                     VRSGDVVFGDVDGVVIIPQERAAEVIARAQEKVRGEDNTRAELEQGRRLAEVFAKYRI
                     L"
     gene            5748..6212
                     /locus_tag="VD791_13815"
     CDS             5748..6212
                     /locus_tag="VD791_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HXV09105.1"
                     /translation="MLNLVRDFFDRHLATPEAGSDERHTIELATAALLVETARLDGEI
                     GPAEREAILGAVRAKFGLDPREADTLVRLAEEEARTANDYYQFTSLINKRFTLEQKER
                     VIELMWQVAYADATLSAHEHHLMRKIGDLLYVPHGNYIAAKMRARDAAARSC"
     gene            6287..>7101
                     /locus_tag="VD791_13820"
     CDS             6287..>7101
                     /locus_tag="VD791_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXV09106.1"
                     /translation="MMNSIAVPRTHRAELANIFARDGFVVPDYRVPDDLLRQMQRSVE
                     KLIAENRGVRPEHLVLRWGGGERALPTHQAFLEYVKTPRILDLVEEVLGPDIVCWGAH
                     VFCKPAGTGLAIPWHQDGQYWPIRPLASCTVWIALDDSTLENGCLRIVRGSHHMRTIY
                     HHRQDERPDHALDQRIDEAEFDTAQIVDVQLRAGEMSIHDVYAIHGSRPNTSPKRRVG
                     FAIRYMPATSLYDRTIKMTGASRGLRQDMSRRPIYLVRGRDRAGNDFECGQDVP"
BASE COUNT         1164 a         2470 c         2372 g         1095 t
ORIGIN      
        1 acatcagctc accctgccgc acttccaggc tcacgccggt cacggcatgc gtgcgatcgg
       61 cgccgggcgg caggcgcacg gtcagccctt cgacgcgcag cgcgggtgcg gtcacggccg
      121 gtctccacgt ttggagaaac gcgggtccag cgtatcgcgc aggccgtcgc cgaggatgtt
      181 caccgcgagc acggtgagcg ccaagaacac accggcgaac agcacgttgt gcgggtactg
      241 cgtgaactgc agccgcccct cggccatgat gttgccccag gtcggcacat cggtcgaaag
      301 ccccacgccg aggaagctca gaatcgcctc caccaggatg gcgaaggcgc agatgtaggt
      361 gccctgcacg atcagcggcg ccaccgtgtt cggcagcaca tggcggtaca gcagtcgcag
      421 cgtcgtcgtg cccaccgaca ccgccgcctc gacgtagggc tcctcgcgga tcgacagcac
      481 cagcgcgcgc gtgagccgca ccacgcgcgg gatctccggc accgtgatgg ccaggatcac
      541 ggtcggcacc gatgggcgcc acagcgccac cagggcgatc gccaggagga tccccgggat
      601 cgccatcagc ccgtccatca cgcgcatcac caccgcgtcc agccgccgca ggaagccggc
      661 ggtcaggccg acgatcagcc cgaagaacag gctgagcagc gccacggcga tgcccaccac
      721 cagcgacacg cggccgccgt acagcacgcg gctgtagatg tcgcgcccgt agctgtcgct
      781 gcccatccag aagcggtgct cgaagctctc gccggtgagc gtcacgactt cggcgcgtgc
      841 gcccggcagc agatcgcggt ttaccggatc gatcagcgtc ggatcgacgg tgccgagcca
      901 gggcgcgagc accgacacca gcgtgagcag caccagcagc gccccgccga tgcgcaccga
      961 ccagttgcgc agcagccggc gccaggcggc cgcgcgcggc gcgggcgcgg ggactgccgc
     1021 ttcggctgtc agctcggcga gcggcgtgtc catcagtagc gaatgcgtgg atcgagcgcc
     1081 aggtaagcga gatcgatcgc caggttgatc agcacgtaag ccaccgagaa cagcaggatc
     1141 acgccctgga tggtcgggaa gtcgcgcgcc agcacggcgt ccacggtcag ccggcccagc
     1201 ccggggatcg catacacgct ttcggtcacc accacgccgc cgatcagcag ggcgaggccg
     1261 atgccgatca cggtcacgat cggcacggcg gcgttcgcca gcgcgtgccg gatcagcacg
     1321 cggcgctcgg tcaacccctt ggcgcgcgcg gtgcgcacga aatcctcggt gagcgcctcc
     1381 gacacggcgg cgcgcgtgac gcgcgcgatc agcgcgatgt agatcaccga cagcgtcagg
     1441 ctgggcagga tcagatgacg aatgaacggt ccgaagccct ccgccagccg ccggtagcct
     1501 tgcaccggta gccagccgag ctcgagcgat accagccaga tgagcagata ggccagcacg
     1561 aacaccggga tcgaaaagcc cagcgtggag aagcccatca gcgcgcggtc cagccagccg
     1621 ccgtgacgcc aggcggcgag cacgcccagc ggcaccgcca ccgccaccgc cagcacgatg
     1681 gtgcacaacg ccagcgcgac ggtgggctcg aggcgctgga cgatcaactc cgccacgctc
     1741 ttgcggaaga agaacgattc gccgaggtcc ccctgcagcg cgttgccgaa ccagatgccg
     1801 aactgcacca ccagcgggcg gttcagcccg aggctttcgc ggatgtgctc gatctgctcg
     1861 gtggtagcta tgtcaccggc gatcaccgcc gccggatcgc ccggcgtcag gcgcagcagc
     1921 aggaatacca ccaccgccac caccagcagg acggggatgg tggcgagcag gcgctggagc
     1981 aggaatcgga tcatcgatca cgctttgtcg atgttccaaa acaccagaaa atacccggtc
     2041 acgaacccgg tcacgctctt gcgggcggcg agcgggatct ggaactcgcc gatcggcacg
     2101 tgcgtgacga tttccatggc gcgggcctgg atctgctcgg cgatggactt gcgcacggca
     2161 tcgtcggcgg tgcgtgcgaa ggcgtcgcgc agcttctcca gctcggcgtc gcagggccag
     2221 ccgaaccagg ccttgtcgca gccggcattc aacgcgttca tggcgacggg attcaatccg
     2281 tccacggagg cccagaaagt gatgaagatg ttccagccgc cctttgccgg aggatccttc
     2341 ttggcgcggc gcgacaccag cgtctgccag tccatggcct gcatgtccac cttgaagccg
     2401 gcctggcgca gcagctgcgt ggcgaccacg ggaaacttcg cggcaggcgc cacgtcggcg
     2461 ggctgcatca gcacgatcgg cgtgccgtcg tagccggact ccttgagcag agcctgcgcg
     2521 cgcttcatat cgggcttggc gatcagttcc atccccttgg acgtctcgta acgcgtcttg
     2581 cagggataga cggaaaaaca ggtccggtac atctcgggaa tgccgatctg ggtgcgcagg
     2641 aaagcgggct ggttcagcgc cgccatcgcc gcacgccgga ccttggggtt gtcgaacggg
     2701 ggctgcagat gattgaagcg cagcatcgcc gcgtagccca ggggattggg ctcgacgatc
     2761 cggatgttcg gatcggtctt gaacgcggcg tacgactcgt gggcggggta ctcgatgatg
     2821 tcgatctcgc ccgcggccaa ggcgttggcc tgggtctgcg gatccttgat gatgacccac
     2881 tcgacccggt ccactttcgc caccttgccg ccggccgtgc cgctggccgc ttcgccgcgc
     2941 gggcggtagc gcgtgttctt ccggtatacc acccgctcgc ccggcttcca ttcgtccttg
     3001 ctgaacacga acggccccga gccggtgtag tcgtcgatct gcctgtcggc cggcgtgtcg
     3061 gccacgcgct tgggcaggat gaacggcacg tacgagctgg gcttgcccag cgactccagc
     3121 accaggccat agggttgcgt gagcttcagg cggaaggtgc gcgcgtcgac cgcttccagc
     3181 gcctgcgtgt agcgcaacag catctcgccc atgctgtcgc gcttcgccca gcgcgtgagc
     3241 gaagcgacca cgtcggcgct ggtgaccggc ttgccgtcgt ggaactcgag cccctcgcgc
     3301 agcgtgaagg tccaggtctt gcggtccttg ctcacctcgt gccgctccac catctgcggc
     3361 cggatctggt tcctggcgtc catcccgtag agcgtgtcgt agatcatgta gccgtgattg
     3421 cgcgtgatat aggccgtggt ccagccgggg tcgaggatgg tgaggttcga gtgcggggtg
     3481 acgcgcacca ccttctcgct gcgcgcgtgc agcggcaggg cggcgcacag ggcggcgagc
     3541 atgacggctg ccggccggaa ccagggtttc atcgtgtcct cctcgaagta attcggtgcg
     3601 ctcgcgggtc gaaacccctc tccccttgcg ggagaggggc aggggtgagg ggtagctcca
     3661 gtctagccgc gccgccggcg cagcgcccgc aggtcctcca gctcccaggg ccggatcgcc
     3721 agcagcaggc ttgccgggcg gcgctcgccc agcgccaggt gcgcgtcggc gtggtgcagg
     3781 tcggcgaact cgttggcgat gcggcgcagc cgggcgatca ccgcctcgct ggaggccttc
     3841 gacagcatcc cgttgaccag cagcatggtc tcgccgggac ggtcgaagct cgagctgaag
     3901 tactcgcgct gcgcgcgcgc cttgaagtac tgctggatcg ggccgttcgg cagccaggtg
     3961 aaggcgcgcg tgacgcgcag cttgatgcgg ttgttgggga gcagctccag gaacttgatg
     4021 cggtcgagct tgagcagcag gccgatgcac tcggtcttgc tgagcgtgta ggtctgcacc
     4081 atctgctcga gcgtcatgtg gctgagcgcg cacagcgcga ccgccagcag cttggggtcg
     4141 gccatgatgg tgcgctcctg ctccggggtg agctgcgaca cctcgtcctt gccgccgtcc
     4201 accgcccgcg ccagctcgcc gatgctggtg cgggccagct cgcagatctg ctcgaagcgc
     4261 tccagcgtga agctgccggt ggcgaacagg cgcttgacgc tgggctcgct cagccgcagc
     4321 gcctgtgcca cctgggcgta ggtgatggcg cgcgccttga gggtgcgctt gagcgcctcc
     4381 accatggcgt cggtttgctg catgcggcct cccccttgga agtatcgcca tttgataccg
     4441 gacggcgagc cggcggcaac ccccagtgaa ccgcttcaca ccggccgata cggcgtgcag
     4501 gatggtctcc acgcgggcgg caaacgcccc ggcacgagaa accgacaggg aggagatcca
     4561 gatgagaacc gacacgctct ccggcgccta tgccaccaag ctcggccgtc ctgcgcaacc
     4621 ggcccttcgc ccggcgtcct cgttgcccct gcgccctcgg ccgctgccgc ccgcgcgccc
     4681 caccgagccg ccgctgcccg attccgaggc cctgttcatc gccctgcgcc gcagcctcgc
     4741 ctcctgccgg gacacgacgc agctgcggat gctcggccgc ctgacgcgcc cttgaggcgc
     4801 gatccaaaaa agacaactcc ccgcctctca cgaggcgggg tggcgcgaag cgccggggcg
     4861 gtgtggtgtg aatctatccc tacaaaatcc gatacttcgc gaacacctcc gccaggcgcc
     4921 gcccctgctc cagctcggcc cgcgtgttgt cctccccgcg caccttctcc tgcgcccgcg
     4981 cgatcacctc ggcggcgcgc tcctgcggga tgatcaccac gccgtccacg tctccgaaca
     5041 cgacgtcgcc gctgcgcacg tggacgccgc cgcaatccac cggggtgtcc cgctccatca
     5101 tgcgcgcccg tcccttggtg tcgagcggcc cgatgccgcc gtggaacacc gggaacccca
     5161 tctcccggat ctgccgcgcg tcgcgcacca gcccgtcggt gacgcacccg gcggcgccgc
     5221 gcgcccgcga cgccgtggac aacagctcgc cccacggggc gatgcgatcc gtcggccccc
     5281 cgcaggcgag caccacggcg tccccgggct tcaggtcgtc caccagcgcg atctccacct
     5341 cgtaggggtt ttcgccctcg tgcactcggt acaccggcat gtacaggccg gtgcgcgctc
     5401 ggccgaccag caccagagac tcgtcgagcg gccgcacgaa gggcttcatc gcccgggccc
     5461 ccagtccgag gctgtccatg acgtcggaca ggaccgcggc gctcagcgcg cgcaacgatg
     5521 ccagatccat ctcttgcatg ccttcctccc tgttgtttcc caggcgcccg gcgatatggt
     5581 ggcgcaggtg ccacgtgccc gttcgtcggc cttcggcgca gtacggtagc cgattgtgcc
     5641 ggcttgcatg atacggttgg aactcctatc cgtggggtgc atcgaagtgt gaccggttgc
     5701 gccgcgagcc gtgtgctccg cgcgccatcc atcgcatatc cgaaccgatg ctgaacctcg
     5761 ttcgcgactt cttcgacagg cacctcgcga cacccgaagc gggtagcgac gagcggcata
     5821 ccatcgaact cgccaccgcg gcgcttctcg tggagaccgc gcgcctggac ggcgagatcg
     5881 ggccggcgga acgcgaggcc atcctgggcg cggtgcgcgc gaagttcggc ctcgatccgc
     5941 gggaggcgga cacgctggtg cgcctcgcgg aagaggaagc gcggacggcg aacgactatt
     6001 accagttcac gtcgctcatc aacaagcggt tcacgctcga acagaaggag cgcgtgatcg
     6061 agctcatgtg gcaggttgcc tacgccgacg cgacgctgag cgctcatgag caccatctca
     6121 tgcgcaagat cggcgatctc ctctatgtgc cccacggcaa ctatattgct gcaaaaatgc
     6181 gcgcccggga cgcagccgcg cgcagttgct gaggcggctc gacgcggcgt agtccgtcca
     6241 gctaaacttc tacgctgtct cgctgtgcgg cgccaggagc atccccatga tgaactcaat
     6301 cgccgtccct cgcacccatc gagccgagct ggcgaacatc ttcgcgcgcg acggtttcgt
     6361 cgtgccggac tatcgggtgc cggatgatct gctgcgccag atgcagcggt cggtggagaa
     6421 gctgattgcc gagaaccgcg gcgtgcgacc cgagcacctg gtgctgcgct ggggcggcgg
     6481 cgagcgcgcg ctgccgacgc accaggcgtt cctggagtac gtgaagacgc cccggatcct
     6541 cgatctcgtc gaagaagtgc tcgggcccga catcgtctgc tggggcgccc atgtgttctg
     6601 caagccggcc ggcacggggc tggcgattcc ctggcaccag gacgggcagt actggccgat
     6661 ccgcccgctg gcaagctgca cggtctggat cgcactggac gactcgaccc tcgagaacgg
     6721 ctgcctgcgc atcgtccgcg ggtcgcatca catgcggacg atctatcacc accgtcagga
     6781 cgagcgcccc gaccacgcgt tggaccagcg tatcgacgag gccgaattcg acacggccca
     6841 gatcgtggac gtgcagctgc gggccgggga gatgtcgatc catgacgtct acgcgatcca
     6901 cggctcgcgc ccgaacacat cgcccaagcg cagggtgggg ttcgccattc gctacatgcc
     6961 cgctacgtcc ctgtacgacc ggacgatcaa gatgacgggc gcgtcgcgcg gcttgcgcca
     7021 ggacatgtcg cggcggccga tctacctggt gcgcgggcgg gatcgggcgg gcaacgactt
     7081 cgagtgcggg caggacgtgc c
//
LOCUS       DASPGE010000420         7286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1167244, whole genome shotgun sequence.
ACCESSION   DASPGE010000420 DASPGE010000000
VERSION     DASPGE010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167244"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VD791_13825"
     CDS             complement(<1..402)
                     /locus_tag="VD791_13825"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HXV09107.1"
                     /translation="MNADLDGYQEVPMALSTAGSGELRVRFNRHDNSFEYKLTYEDLE
                     GIVTQAHIHFGRPGVNGGISVWLCGTSANPGPAGTPTCPGPNSGEVSGTITSAQVVGP
                     EAQGIAPGEFDELVRAMREGATYANVHTDTRP"
     gene            835..1365
                     /gene="greB"
                     /locus_tag="VD791_13830"
     CDS             835..1365
                     /gene="greB"
                     /locus_tag="VD791_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HXV09108.1"
                     /translation="MRAEDDEESAVPPIPAGSKNYITPAGFQRLKDEALQLLNNERPE
                     LVKVIQWAAANGDRSENADYIYGKRRLREIDRRIRFLTKRMEAAEVIDPSAREATDQV
                     FFGATVTLADQDGRETRYTIVGIDEADATRGRISWVSPLARALLKRHEGERIVFQAPG
                     GDQVLDIVAVEYKPLD"
     gene            complement(1463..2266)
                     /locus_tag="VD791_13835"
     CDS             complement(1463..2266)
                     /locus_tag="VD791_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXV09109.1"
                     /translation="MRYSDYSTLKLSRPEPGILEIAMGEPGRLSVADAQGHRELTEIW
                     REVDADPETSVAIIRGIGKGYSGGGSLDLVEQMSEDFDTRARVWREARDLVYNLINCS
                     KIVVSAMHGPAVGAGLVTGLLADISIAARNARIIDGHTRLGVAAGDHAAVVWPLLCGM
                     AKAKYYLLLCEEVSGEEAERIGLVSLCVDESELTAKAFEVARKLAQGSQSALRWTKYS
                     LNNWLRMAGPTFDTSLALEMLGFTGPDVREGAASLRAKRPPKFDPACPF"
     gene            complement(2387..3097)
                     /locus_tag="VD791_13840"
     CDS             complement(2387..3097)
                     /locus_tag="VD791_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HXV09110.1"
                     /translation="MIDLYYWPTPNGHKITIFLEETGLPYRIVPVNIGRGEQFEPEFL
                     AISPNNRMPAIVDRTPADGGAAIPVFESGAILVYLAAKTGKFLPRDTRGWVQVLEWLF
                     WQVAGLGPMAGQTHHFLHYAPERIAYPTQRYVMETSRLYAVLDKRLADREYLAGDYSI
                     ADMACYPWIVPHKRQEQNLDEFPDLKRWFEAIHARPAVVRAYDKGKAITTQPTVDDEA
                     RKFLFGQSAATVRGVGPR"
     gene            3367..4746
                     /gene="gorA"
                     /locus_tag="VD791_13845"
     CDS             3367..4746
                     /gene="gorA"
                     /locus_tag="VD791_13845"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HXV09111.1"
                     /translation="MAQHYDLITIGGGSGGVAVSNRAGSYGARCLLVEKGRLGGTCVN
                     VGCVPKKIMWNGAQLAHALEDAADYGFRLTGREFDWAALKRERDRHVRDLNGHYAKSL
                     ASNKVEMIAGEARFVGPRSIDVAGRRCSADHVVIATGGHPIVPDLPGAGYGITSDGFF
                     ELERLPRKVAIVGAGYVAAELSGVLHALGSELTVVLRREQLLMSFDPMLRETLMEHMR
                     ADDIEFVTNTQLVGLEKDAHGGLTLLCSSGTRIGGFDTVIWAIGRAPNTAGLDLGATG
                     LAADELGFLPTDAFQETRVAGIYAIGDVAGRAALTPVAIAAGRRLADRLFGGMRERKL
                     VYENIPTVIFSHPPIGTVGLSEPEALQRYPADQLKVYQARFTPMSHAFTTRKVKTAIK
                     LIVAGPEETVVGCHIIGPSADEMLQGFAVAVRMGATKRDFDDTVAIHPTVAEELVTLR
                     GARSAALPL"
     gene            4827..6227
                     /locus_tag="VD791_13850"
     CDS             4827..6227
                     /locus_tag="VD791_13850"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HXV09112.1"
                     /translation="MNQASANEAKEVQLGHYDPYQLDPKHILEPPTEMGAILSKIGPG
                     IVLSASIVGSGELIATTTLGAEVGYTVMWLIIFSCLIKALIQSFLGRYTIAKGETGLE
                     AFNRIPGRIGRINWVVWAWAAMVAVTLFQICAMFIGVSQAMRLITGIEVVWWVLAFFV
                     LTLVLLLGGAYHRIERLAMIKVGLFTLITLLSAVVLTRMPQYFSWGDVLQGMTFKLPE
                     KGLATALAVFGITGVGATELYMYTYWCVEKGYARYTGPHEDSEAWRRRARGWVRVMNV
                     DVIFSMIIYTVATIAFYLLGAGILHGMGQVPATSDMIPILSNIYTETLGGWAKWLFYI
                     GAIVVLYGTIFAATAGHSRMFADFMRLQGRFEHDDMKARNRWRDIFIVVLTVIPVIMF
                     FVFGEKPVAMVKWGGVAQAWTLPILSLGTVYLVRRYLPKEMSATPLGNALLWIGAIVI
                     TGFVLVSEARRWGLFG"
     gene            6283..6630
                     /locus_tag="VD791_13855"
     CDS             6283..6630
                     /locus_tag="VD791_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXV09113.1"
                     /translation="MSIERKKVGPRLSQAVVHGDTVYLAGIVADHPVPEVAAQTGQIL
                     DQIDKLLAECGSNKSKLLMATIWLADIRYYDEMNSVWDKWVAPGQTPARACVEARLAQ
                     PKYLVEIRVVAAR"
     gene            6790..>7286
                     /locus_tag="VD791_13860"
     CDS             6790..>7286
                     /locus_tag="VD791_13860"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HXV09114.1"
                     /translation="MEVLFLAVLILLNGVFAMSEIAVVSARKTRLQHWADEGRAGAAA
                     ALRLANDPGRFLSTIQVGITTIGILSGAIAEASVAQTLAASLAQVPAVAPYSEGIAIG
                     IVVVAITYLSVVFGELVPKRLALINPEALSSVIAQPMQWLSRISSPLVRFFNLSSEVV
                     LSLLGA"
BASE COUNT         1178 a         2442 c         2422 g         1244 t
ORIGIN      
        1 cgggcgtgtg tccgtgtgca cgttcgcgta cgtcgcacct tcacgcatcg ccctgaccag
       61 ttcgtcgaac tctcctggtg cgatgccttg cgcttccggg ccgaccacct gggcggaggt
      121 gatggtgccg gagacctcac ccgagttcgg ccccgggcag gtcggggtgc ccgccggacc
      181 cggattggcg ctggtgccgc acagccagac gctgatgccg ccgttgacgc cggggcgccc
      241 gaaatggatg tgggcctgcg tcacgatccc ctcgagatct tcataggtca gcttgtattc
      301 gaacgagttg tcgtggcgat tgaacctgac gcgcagctcg ccgctgccgg ctgtggacag
      361 cgccatcgga acttcctggt agccgtcgag atctgcgttc aaagaccgcc ttctcccccc
      421 gtccgcccag gcatcctgaa ggctggggac ggccaacacg cccgctaccc ccgcgagcaa
      481 tgcgaaacgt aagactttca tcgtcgtcct ccttcgcatg catcatcgat gatgcagttt
      541 tccacgttgc gcaacgatct tgcggagatg gcaagacact acgctcgccg tgaagccggc
      601 gtcaagatcg aaacggcttg tcgtcatcga ctgctcgtcg ccttcgtgcg acgcgcgcgt
      661 cgatcttcga tggcgatgaa gtcgccgtgg agacttcttc gatccctcac atcggcgaat
      721 gatcgcaacg agtccgccgc aacctcgcag agccgcatgc gtcggcgcaa ggcctataat
      781 tctcctgtgc cgcaccagcc atcttcagcc cgaaacggac agcctgcgcc gaccgtgcgc
      841 gccgaggacg acgaggagtc cgccgtcccg cccatccccg cgggcagcaa gaactacatc
      901 acgcccgccg ggttccagcg cctgaaggac gaagcgctgc agctgctcaa caacgagcgc
      961 cccgagctgg tgaaggtgat ccagtgggcg gcggccaacg gcgaccgctc ggagaacgcc
     1021 gactacatct acggcaagcg gcggctgcgc gagatcgacc ggcgcatccg cttcctgacc
     1081 aagcgcatgg aggcggccga ggtgatcgac cccagcgcgc gcgaggcgac cgaccaggtg
     1141 ttcttcggcg ccaccgtcac gctggccgac caggatggcc gggaaacccg ctacaccatc
     1201 gtgggcatcg acgaggccga cgccacgcgg ggacgcatca gctgggtctc gccgctcgcc
     1261 cgcgcgctgc tcaagcgcca cgaaggcgag cgcatcgtgt tccaggcgcc cggcggcgac
     1321 caggtgctgg acatcgtcgc cgtcgagtac aagccgctgg attgaatgcg ccgttgaggc
     1381 gcgtcttaag aaaaatcccc acctctcaat gcagcgagtg ccggcgcggc cgggcggggc
     1441 ggtagattgc tgcgcgcttc gctcagaacg gacaggcggg atcgaacttc ggcgggcgct
     1501 tcgcgcgcag tgacgccgcg ccttcgcgca cgtcgggacc ggtgaagccc agcatctcca
     1561 gcgccagcga ggtgtcgaag gtcggcccgg ccatgcgcag ccagttgttc aggctgtact
     1621 tggtccagcg cagcgccgac tgcgatccct gcgccagctt gcgcgccacc tcgaacgcct
     1681 tggcggtgag ctcgctctcg tccacgcaca gggacaccag gccgatgcgc tcggcttcct
     1741 cgccgctcac ctcctcgcac agcagcaggt agtacttcgc cttggccatg ccgcacagca
     1801 gcggccacac caccgccgca tggtcgccgg cagccacgcc cagccgcgta tggccgtcga
     1861 tgatgcgtgc gttacgcgct gcgatggaga tatcggcgag caagccggtg accagccccg
     1921 ctcccaccgc cggtccgtgc atggccgaga ccacgatctt ggagcagttg atcaggttgt
     1981 agaccaggtc gcgcgcctcg cgccacacac gcgcgcgcgt atcgaagtcc tcgctcatct
     2041 gctccaccag gtcgaggctg ccgccgccgg agtagccctt gccgatgccg cgaatgatcg
     2101 ccaccgaggt ctccgggtcg gcgtccactt cgcgccagat ctcggtcagc tcgcgatgcc
     2161 cctgcgcgtc ggccacgctc aaccggccgg gctcgcccat cgcgatctcg aggatgcccg
     2221 gctcggggcg gctgagcttg agggtgctgt aatcggagta acgcatgctg gtctccggcg
     2281 gagtgcgacg cgctggtgct tggatgggcg atttcggggt cgaaactggc ccgctaaggc
     2341 cgaaaaacgc ccgcctttgg gaacagagtg cgcaaatctc agatacctag cgtggtccga
     2401 ccccacggac ggttgcggcg ctttggccga acaggaattt cctcgcctcg tcgtcgacgg
     2461 tcggttgggt ggtgatcgct ttgcccttgt cgtaggcgcg caccaccgcc gggcgggcgt
     2521 ggatcgcctc gaaccagcgc ttcagatcgg ggaactcgtc caggttctgc tcctggcgct
     2581 tgtgcggcac gatccaggga tagcaggcca tgtcggcgat ggagtagtcg ccggccaggt
     2641 actcgcgatc ggcgagccgc ttgtcgagca ccgcgtacag gcgcgaggtc tccatcacgt
     2701 agcgctgtgt cgggtaggcg atgcgctccg gcgcgtaatg caggaaatgg tgcgtctggc
     2761 cggccatcgg gcccaggccg gcgacctgcc agaacagcca ctcgagcact tgcacccagc
     2821 cgcgggtgtc gcgcgggagg aacttgccgg tcttggccgc gagatagacc aggatggcgc
     2881 ccgactcgaa caccgggatg gccgcgccgc cgtcggccgg cgtgcgatcc acgatcgccg
     2941 gcatgcggtt gttcggcgag atcgcgagaa actccggctc gaactgctcg ccccggccga
     3001 tgttcaccgg cacgatgcgg tagggcagcc cggtctcctc caggaagatg gtgatcttgt
     3061 gcccgttggg cgttggccag tagtagaggt cgatcatggt cgcagaagag gaatgggtca
     3121 cggaatgata cgctcggcgc cgcgcgaaca ggtcgagaaa catgcgctcc gagtccaccg
     3181 actcgcagaa gccgacgcgg cgtgcccacg ccggttgcgc ccgccaccag tgccttgtac
     3241 cccgccatcg cgttgcccac ccttgtccga aggggaatat cttgagggat atgcccccgt
     3301 tcgggcaaac tgcgcggcac ggcagcaact gcgctttcct ttcgaactca tcggctggcg
     3361 ttctcaatgg cacagcatta cgatctgatc accatcggcg gcggcagcgg cggcgtcgcg
     3421 gtctccaacc gtgccggcag ctacggcgcg cgctgcttgc tggtcgagaa gggtcgcctg
     3481 ggcggcacct gcgtcaacgt cggctgcgtg ccgaagaaga tcatgtggaa cggcgcgcag
     3541 ctcgcccacg cgctggagga cgcggccgat tacggctttc gcctgacggg ccgcgagttc
     3601 gactgggccg cgctcaagcg cgagcgcgac cggcacgtgc gcgatctgaa cggccattac
     3661 gccaagtccc tggcgagcaa caaagtggag atgatcgccg gcgaggcgcg cttcgtcgga
     3721 ccgcgcagca tcgacgtcgc cggccggcgc tgcagcgccg atcacgtggt catcgccacc
     3781 ggcggccacc cgatcgtgcc cgatctgccc ggtgccggct acggcatcac ctcggacggc
     3841 ttcttcgagc tggagcggct gccgcgcaag gtcgccatcg tcggcgccgg ctacgtcgcc
     3901 gctgagctct cgggcgtgct gcatgccctg ggcagcgaac tcacggtggt gctgcgccgc
     3961 gagcaactgc tgatgagctt cgacccgatg ctgcgcgaga cgctgatgga gcacatgcgc
     4021 gccgacgaca tcgagttcgt gaccaacacg cagctcgtcg gtctggagaa ggacgcgcac
     4081 ggcggcctca ctctgctgtg ctccagcggt acgcgaatcg gcggcttcga cacggtgatc
     4141 tgggccattg gccgcgcgcc caacaccgcc ggtctcgacc tgggtgccac gggactcgcc
     4201 gccgacgagc tgggtttcct gccgaccgac gcgttccagg agacccgcgt cgcgggtatc
     4261 tacgccatcg gtgacgtcgc cgggcgcgcc gcgctgacgc cggtggccat cgccgccggc
     4321 cggcgcctgg ccgaccgctt gttcggcggc atgcgcgagc gcaagctggt gtacgaaaac
     4381 atccccaccg ttatcttcag ccatccgccg atcggcaccg tcggattgag cgagccggag
     4441 gcgctgcagc gctaccccgc cgaccagctc aaggtgtacc aggcgcgctt cacgccgatg
     4501 tcccacgcct tcaccacgcg caaggtcaag actgccatca agctgatcgt cgccgggccg
     4561 gaggagacgg tggtgggctg ccacatcatc ggcccgagcg ccgacgagat gctgcagggt
     4621 tttgcggtgg cggtgcgcat gggcgcgacc aagcgcgact tcgacgacac cgtggcgatc
     4681 catcccaccg tggcggagga actggtcacc ttgcgcggtg cgcggtcggc ggccctgccg
     4741 ctttgatgtt accttacacg ctccggcgca agcccgccgg ccgaaccagc cgagttcggg
     4801 cataacaaac cacagggggg aggagcatga accaggcttc cgccaacgag gccaaggaag
     4861 tccaactcgg gcattacgac ccgtaccagc tcgatcccaa gcatattctc gagccgccga
     4921 ccgagatggg cgcgatcctc tccaagatcg gcccgggtat cgtgctgtcc gcatccatcg
     4981 tcggctcggg cgagctgatc gccaccacca cgctcggcgc cgaggtcggt tacacggtca
     5041 tgtggctgat catcttctct tgcctgatca aagcgctgat ccagtcgttc ctgggtcgtt
     5101 acaccatcgc caaaggcgaa accgggctgg aggccttcaa ccgcattccg ggccggatcg
     5161 gccggatcaa ctgggtggtg tgggcatggg ccgcgatggt ggcggttacg ctgttccaga
     5221 tctgcgccat gttcatcggc gtgtcccagg cgatgcggct gatcaccggg atcgaggtgg
     5281 tgtggtgggt gctggcgttc ttcgtgctga cgctggtgct gctgctcggc ggcgcgtacc
     5341 accgcatcga gcgcctggcg atgatcaagg tcgggctgtt caccctgatc acgctgctgt
     5401 cggccgtggt gctgacgcgc atgccgcagt acttctcctg gggcgacgtg ctccaaggca
     5461 tgaccttcaa gctgccggag aagggtctcg ccaccgcgct ggcggtgttc ggcatcaccg
     5521 gcgtgggcgc caccgagctg tacatgtaca cctactggtg cgtggagaag ggctacgcgc
     5581 gctacaccgg cccacacgag gacagcgaag cgtggcgccg ccgcgcgcgc ggctgggtgc
     5641 gggtgatgaa cgtggacgtg atcttctcca tgatcatcta caccgtggcc accatcgcct
     5701 tctatctgct cggcgccggc atcctgcacg gcatggggca agtgccggcc accagcgaca
     5761 tgatcccgat cctgtcgaac atctacaccg agacgctggg cggctgggcc aagtggctgt
     5821 tctacatcgg cgctatcgtc gtgctctacg gcaccatctt cgccgccacc gccggccact
     5881 cgcgcatgtt cgccgacttc atgcggctgc aggggcgctt cgagcacgac gacatgaagg
     5941 cgcgcaaccg ctggcgcgac atcttcatcg tggtgctgac cgtgatcccg gtgatcatgt
     6001 tcttcgtctt cggcgagaag ccggtggcga tggtcaagtg gggcggcgtc gcgcaggcct
     6061 ggacgctgcc catcctgtcg ctgggcacag tgtatttggt tcgccgttac ctgcccaagg
     6121 agatgagcgc cacgccgctc ggcaacgcgc tgctgtggat cggcgcgatc gtgatcaccg
     6181 gcttcgtgct ggtgagcgag gcgcggcgct ggggattgtt cggctgagcc gggcggttct
     6241 ttcttttaag acgcgccttg agggcgcatt ggagcgagag agatgagcat cgagcgcaag
     6301 aaggtcggcc cgcgcctgag ccaggcggtg gtgcacggcg atacggtgta tctggcgggg
     6361 atcgtggccg atcacccggt cccggaggtg gcggcgcaga ccggccagat cctggaccag
     6421 atcgataagc tgctggccga gtgcggcagc aacaagagca agctgctgat ggcgaccatc
     6481 tggctcgccg atatccgcta ctacgacgag atgaattcgg tctgggacaa gtgggtcgca
     6541 ccgggccaga cgccggcgcg cgcctgcgtc gaagccaggc tggcgcaacc caagtacttg
     6601 gtcgagatcc gggtggtggc ggcgcgctag ttcgcgtcgc tcgtcaacgc ggagagatgc
     6661 ctggattgcg ccgctgggcg gctgcctcca ggctgcacgc tcgggaataa gccagcacgc
     6721 acggccgcgc cgccctccgt cggcgggcac tgcggtatcc ttgcagcttc ccaattcccg
     6781 gtacgctcca tggaagtgct ctttctagcc gttctcatcc tgctgaacgg cgtgttcgcc
     6841 atgtccgaga tcgccgtggt ctccgcgcgc aagacccggc tgcagcattg ggccgacgag
     6901 ggccgcgccg gcgccgcagc cgcgctgcgc ctggccaacg acccgggccg cttcctgtcc
     6961 accatccagg tcggcatcac cacgatcggc atcctgagcg gagcgatcgc ggaagcgtcg
     7021 gtggcccaga cgctggcggc gtcgctcgcg caagtgcccg cggtggcgcc ttacagcgag
     7081 ggcatcgcca tcggcatcgt ggtggtcgcc atcacctacc tgtcggtggt attcggcgag
     7141 ctggtgccca agcgcctcgc gctgatcaac ccggaggcgc tttccagcgt catcgcccag
     7201 ccgatgcaat ggctttccag gatcagctcc ccgcttgtgc gcttcttcaa cctgtcgagc
     7261 gaggtggtgc tgagcctgct gggcgc
//
LOCUS       DASPGE010000421         9233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_467418, whole genome shotgun sequence.
ACCESSION   DASPGE010000421 DASPGE010000000
VERSION     DASPGE010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9233
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467418"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VD791_13865"
     CDS             <1..788
                     /locus_tag="VD791_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HXV09115.1"
                     /translation="RLAQGLTVGHLLECSGQGSGGNFGAADAWAQIPDLGHIGYPIAE
                     VSEDGSALITKAPGTGGRVDFDTVRQQLLYEVHDPHRYVSPDVVLDMGTLTLDDLGGD
                     RVAVKGAGGHPRPQRLKILAGYEDGWMGSSVVGFCWPDAWRKAQAAVALVKQSLQEQR
                     VPIDEMLVEYPGLDAFLGPHADVSQRDDLNEVWVRVAVRTQDKRHADGLGRHFPWLAL
                     SGPPYMSGFHGVTPGSQLLGVWPALVARELVESRVDVSLLEVA"
     gene            785..1144
                     /locus_tag="VD791_13870"
     CDS             785..1144
                     /locus_tag="VD791_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV09116.1"
                     /translation="MKVLLARIAHARSGDKADRADIGLFAYDAPSYAVIKREVTAERV
                     AAHFAWLAEGPVERYPLDNVLALKFVLNRALQGGAARSLRSDNLGKTIGAALLRMQID
                     IDPAEASGLPRLHAQAG"
     gene            1505..1747
                     /locus_tag="VD791_13875"
     CDS             1505..1747
                     /locus_tag="VD791_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV09117.1"
                     /translation="MTTLELTLNLPDSLAQEAKAAGLLTPEAIEQMLKRELKRRAGEL
                     LLESARALQAAEPRLSEDEIQAEIDAARAQRRAHRS"
     gene            1719..2159
                     /locus_tag="VD791_13880"
     CDS             1719..2159
                     /locus_tag="VD791_13880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HXV09118.1"
                     /translation="MHSAVRIVLDTNTVVSALLWGGPPYALIEAATEERVELYSSKTL
                     LEELADVLGRPKFARQLARAQRTVADLIEQYRGLVELVEPASVTPSVRDDPDDDHVLA
                     CALAANADAIVSGDAHLQTLASYQGIPILAAAQCRQLLSSESRE"
     gene            2292..3158
                     /locus_tag="VD791_13885"
     CDS             2292..3158
                     /locus_tag="VD791_13885"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV09119.1"
                     /translation="MPLELLIEAVIAGVLLGCFYAAVSVGLSVAFGLLDVPQIAHPAF
                     LVLGAYGAYLLGTYGVDPLLAGALLTPVFFLGGLGMYRVYHAVFERRSDAGLGGLAFL
                     FGVAFIVEVALILAFGVDQRMVEAAYIGRSVALGELRLPIRMLVAFGVALLLTGTLAL
                     YLSRTFTGRAIKAVAQDQTALALMGADPVRIKEWAFGIATATCALAGALLIIVQPVEP
                     GLGRLYIGRTFCVAVLAGLGSMPGTLAAGVILGVAESIVLTSLGASWAPAVAFGLLLL
                     VLAIRPAGLFGR"
     gene            3166..4116
                     /locus_tag="VD791_13890"
     CDS             3166..4116
                     /locus_tag="VD791_13890"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXV09120.1"
                     /translation="MRGWLQFGIGAAALLGFAFLATQAIDNEYVFFAGYVVLQFVVLA
                     TAWNILGGYAGYVNFGSAAFFGIGAYIAVALFKAFEAPLPAQLAAAMAVTGLMGFAVG
                     ALTMRLRGVFFSIATVAIAVICETLVVNWGFVGGARGTAVLQPPAPALFASYTRMLFV
                     AMALFAVLAVAVARYIETSWIGRGLRALRDSEEAAESLGVPTLNLKLFAATASGALMG
                     LAGAPLALYMSYIEPTSAFSLNYAVSALAMPIVGGTGSWLGPVIGALLLGSTQQIVTV
                     TVSSELNVLIVGLLLVAFVVVAPQGIVGLLQHLLRPRGAK"
     gene            4113..4886
                     /locus_tag="VD791_13895"
     CDS             4113..4886
                     /locus_tag="VD791_13895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV09121.1"
                     /translation="MTAALLDVQSVSKRFGGFAALTDVSLGVRGGERLGIIGPNGSGK
                     TTLINCICGALRGHRGTIVFDGHDVTGLAPYQRTRLGIARSFQIPRPFASMSLVDNVA
                     VPLEYVARQDLTNRDPAPRHGKRWGPLAAADIRAEALAILDQMGLQGGADAPAATLTQ
                     VGLRKLELARAMAARPRLLILDEVMAGLSSTEVDEVLTLLFTLSERGVTILMIEHIMR
                     AIMRFSERVVCLDAGRVMAEGPPATIAADPMVRRIYLGA"
     gene            4879..5607
                     /locus_tag="VD791_13900"
     CDS             4879..5607
                     /locus_tag="VD791_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV09122.1"
                     /translation="MLKLTVQDLEAGYGAVRALHGVSLEVRQGETVALLGTNGNGKST
                     LMKCIMGLLRPARGSVVLELDGNRIDLTGKSPAQIVGLGVAMVPEGRRLFPRLTVQEN
                     LLLGAFRQAARPSIRDNLALCYEAFPILRDRPDQLAGSLSGGQQQMLAIARAIMSSPK
                     LLLVDEPSVGLSPMLVRQTIDVIRDLKQRAGLTVLMAEQNFHEASRIADRGYIIVHGE
                     IAFEGRDLEELQRSDLVKSYYLGQ"
     gene            5633..6535
                     /locus_tag="VD791_13905"
     CDS             5633..6535
                     /locus_tag="VD791_13905"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV09123.1"
                     /translation="MATLPQIGWIGAGRMGVPMAGFLLQAGYPVRVYSRTAASRQKLV
                     ARGATEAQSVAECARGAEIVFCSVSDDAALREVALGPGGVLASLAPGAVFAETSTVSV
                     EVSEQVAQEAARRGVAYLRLPISGNAAQAQKGELTVLVSGPLDAWNRIKPVCERFSKA
                     QVYLGAGEQARVMKLVVNALVYVFAQAMAEAVTLGRKAGLDWGVMLDALAQSALASPW
                     LKVKAELMKRRDFTPTMTPPLVLKDIDLMMACARAQQVSMPLTALTRQLMQTLVGQGF
                     GDEDYMAAIKLAEKQAGLPSDRVD"
     gene            6548..7810
                     /locus_tag="VD791_13910"
     CDS             6548..7810
                     /locus_tag="VD791_13910"
                     /inference="COORDINATES: protein motif:HMM:NF005566.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXV09124.1"
                     /translation="MRPANPGRALVIGGSIAGLIAGNLLHRAGWDVHVFERASGDLEG
                     RGAGITILPGLVSSFQAAGVDQTERSLGIELPARIALDRAGRAIVERVFAQSMTSWGR
                     LYQALRNVYPADRYHGGMALERVEQHADRVSAVFAGGQRVESDLLIGADGLRSTVRSQ
                     FLPELQPYYSGYLAWRCLADERDLTPEVFAPLFNRYTVCVAPGEQGIGYPVPGPDHSV
                     APGRRQYNVVWYHPVREAEELPRFMTDDRGRCHPNGIPPSLLSSAIRDEMVGIAPGVL
                     APQFAEALRRGKLHFFQPILDIEPQRLAFGRVAIIGDAAFVARPHVAMGVPKGAGDAL
                     ALVQSIAAAGGDVPAGLQRFEAERLRVGRTIVARGRYLGAYMEAQLKSEAERRAAEAQ
                     REPERVMMETAAPLDYDAMAQTATGSTG"
     gene            complement(7791..9161)
                     /gene="hmgA"
                     /locus_tag="VD791_13915"
     CDS             complement(7791..9161)
                     /gene="hmgA"
                     /locus_tag="VD791_13915"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HXV09125.1"
                     /translation="MAEQQGSTGQQKDAAQQKDAPQRKDSGLRYQTGFANEFATEALP
                     GALPLGQNSPQKCPYGLYAEQLSGTAFTAPRGANRRSWLYRIRPAAVHRPFRRVDHET
                     LTSNFASTATPPNQLRWDPLPMPNASTDFVDGLVTMAGNGDPHAQSGCGIHLYAANRS
                     MSGRFFYNADGEMLIVPQQGRLRFATELGLIDAEPQEIVVIPRGVRFRVELLDATARG
                     YICENFGAAFRLPDLGPIGSNGLANPRDFQTPVAWYEDKEGGFELLAKFMGNLWAAGI
                     GHSPLDVVAWHGNHAPYKYDLRRFNVIGSISYDHPDPSIFLVLQSLSDTPGWDSIDFV
                     IFPPRILAMQHTFRPPWFHRNVASEFMGLIHGAYDAKAEGFVPGGASLHNCMSGHGPD
                     AAIFEKASNADLSKHDVIRDTMAFMFESRCVIRPTAFALQSTQLQADYYQCWQGLKKN
                     FNPSTP"
BASE COUNT         1326 a         3074 c         3311 g         1522 t
ORIGIN      
        1 ggcggctggc gcaggggctc acggtcggcc atctgctgga gtgctccggt cagggcagcg
       61 gcggcaactt cggcgccgcc gacgcctggg cgcagatccc cgacctcggc cacatcggct
      121 atcccatcgc cgaggtgagc gaggacggca gcgccctcat caccaaggcg ccgggcaccg
      181 gcggccgcgt cgatttcgac accgtgcgcc agcagctcct gtacgaggtg cacgacccgc
      241 accgctatgt ttcgcccgac gtggtgctgg acatgggcac gctcacgctc gacgatctgg
      301 gcggcgaccg cgtcgcggtc aagggcgccg gcgggcatcc gcgaccccag cggctcaaga
      361 tcctcgccgg ctacgaagac ggctggatgg gcagcagcgt ggtcggcttc tgctggccgg
      421 acgcctggcg caaggcgcaa gcggcggtgg ctctggtcaa gcagtcgctg caggagcagc
      481 gcgtgccgat cgacgagatg ctggtcgagt acccggggct cgacgccttt ctcgggccgc
      541 acgcggacgt ttcgcaacgc gacgacctga acgaagtctg ggtgcgcgtg gccgtgcgca
      601 cgcaggacaa gcggcacgcg gacggtctgg gccggcactt cccctggctc gccctgtccg
      661 gcccgcccta catgagcgga ttccacggcg tcacgcccgg aagccagttg ctcggcgtgt
      721 ggcccgcgct ggtcgcgcgc gagctggtcg agagccgggt ggacgtctcc ctgctggagg
      781 tggcgtgaag gtgctgctgg cgcgcatcgc gcacgcgcgc agcggcgaca aggccgaccg
      841 cgcggacatc ggcctgttcg cctacgacgc gccgagctat gccgtgatca agcgcgaggt
      901 caccgccgag cgcgtggccg cgcatttcgc gtggctcgcc gagggaccgg tggagcgcta
      961 tccgctcgac aacgtcctgg cgctcaaatt cgtcctcaac cgcgcgctgc agggcggcgc
     1021 ggcgcgcagc ctgcgcagcg acaacctcgg caagaccatc ggtgcggcgc tgctgcggat
     1081 gcagatcgac atcgacccgg cggaggccag cggcttgccg cgcctgcacg cacaggcggg
     1141 ttgagacgaa gtgcggcttg cgcgcagtgc cggttgaatc gaccatcggg ctgtacaagt
     1201 cgcccgcgcg gcccgtggcg cgacctggaa gcgggggttt tcgacgctga agtgggtgtg
     1261 ctggttccag cagtggtggc gtggtcaaac gcgtcgctca ccgaggtgac ctgaccatag
     1321 ttgccgcgtg cgtcccttag gtcggcacgg tgcgaactat cgcgggtgta gtagcgcacg
     1381 ccggcgttga ggtcggtggc ggtagctgtt actttttccg tcgtaccact ggtaagatgg
     1441 gtgctggagc gactttgctg ctgctgctgg agggcgtgct ggcacggacc agctgccgga
     1501 ccatatgacg acgctggaac tgactctgaa tctccccgat agcctggccc aggaggcaaa
     1561 ggccgcgggt ctgctcacac ccgaggcgat cgagcagatg ctcaagcgcg aactcaagcg
     1621 acgcgcaggc gaactgctcc tggagagcgc aagagcgctg caagcggccg agccgcgctt
     1681 gagcgaagac gagatccaag ccgagatcga cgccgcgcgt gcacagcgcc gtgcgcatcg
     1741 ttcttgacac caacacggtg gtctcggcgc tgttgtgggg tggtccgccg tacgctctga
     1801 ttgaagcggc aacggaagag cgcgtcgagc tgtacagcag caagacattg ctcgaagaac
     1861 tcgcggatgt gctcggccga ccaaagttcg cccggcagct agcccgggcg cagcgcactg
     1921 tcgccgacct catcgagcaa tatcgcggtc tcgtggaact cgtcgagccc gcgtccgtca
     1981 cacccagcgt gcgggacgac cccgacgacg atcacgtgct tgcctgtgcg cttgccgcga
     2041 acgccgatgc gatcgtgtcc ggagacgcgc acttgcagac gcttgcgagc taccaaggca
     2101 tccccatcct cgccgcggcg cagtgccgac aactcctgag cagcgagtca agagagtaga
     2161 acgtagtaac tgacgctctt gccggcgcac gcgcgtcgtt ctgcgatcga ctagaagagg
     2221 cttcccgtgg cagcgttccg cactgcgttg gtgcccagca gcatgccctt atccgcattc
     2281 ggtgccgatc catgccgctc gaactgctga tcgaagccgt catcgccggc gtgctgctcg
     2341 gctgcttcta cgccgcggtg agcgtgggcc tgtcggtggc gttcgggctg ctcgacgtgc
     2401 cgcagatcgc gcatccggct ttcctggtgc tgggcgccta cggcgcctat ctgctcggca
     2461 cctacggcgt cgatccgctg ctcgccggcg cgctgctgac gccggtgttc tttctcggcg
     2521 ggctgggcat gtaccgggtg taccatgcgg tgttcgagcg gcgcagcgac gccggcctgg
     2581 gcggcctggc cttcctgttc ggcgtcgcct tcatcgtgga ggtggcgctg atcctggcct
     2641 tcggcgtgga ccagcgcatg gtcgaggcgg cttacatcgg ccgcagcgtg gcgctcggcg
     2701 agctgcgcct gccgatccgc atgctggtcg ccttcggcgt ggcgctgctg ctcaccggca
     2761 cgctcgcgtt gtacctttcg cgcaccttca ccggccgcgc gatcaaggcg gtggcgcagg
     2821 accagactgc gcttgcgctg atgggcgccg atccggtgcg gatcaaggag tgggcgttcg
     2881 gcatcgccac cgccacctgc gccctggcgg gcgcgttgct gatcatcgtg cagccggtcg
     2941 agccgggcct gggaagactg tacatcggcc gcacgttctg cgtggcggtg ctggccggcc
     3001 tgggcagcat gccgggaacg ctcgccgccg gcgtgatcct gggtgtcgcc gagagcatcg
     3061 tgctcacctc gcttggcgca tcctgggcgc cggcggtcgc attcgggctg ctgctgctgg
     3121 tgctggcgat ccgcccagcg ggtctgttcg ggcgctgaac gcacgatgcg cggctggctc
     3181 cagttcggca tcggcgcggc tgcgctgctc gggttcgcgt tcctcgccac ccaggccatc
     3241 gacaacgagt acgtcttttt cgccgggtac gtggtgctgc agttcgtcgt gctcgccacc
     3301 gcctggaaca tcctgggagg ctacgccggc tatgtgaact tcggctccgc cgccttcttc
     3361 ggcatcggcg cctacatcgc ggtagcgctg ttcaaggcgt tcgaggcgcc gctgcccgcg
     3421 cagctcgccg ccgcgatggc ggtcaccggc ctgatggggt tcgcggtcgg cgcgctgacc
     3481 atgcgcctgc gcggcgtgtt cttctccatc gccacggtgg ccatcgccgt catctgcgag
     3541 acgctggtgg tcaactgggg tttcgtcggc ggcgcccgcg gcaccgcggt gctgcagccc
     3601 cctgccccag cgctgttcgc ctcgtacacg cgcatgctgt tcgtggccat ggcgctgttc
     3661 gcggtgctgg cagtggccgt cgcgcgctac atcgagacct cctggatcgg ccgcgggttg
     3721 cgtgcgctgc gcgacagcga agaggcggcc gaatcgctgg gcgtgccgac gctcaacctg
     3781 aagctgttcg cggcgaccgc ctcgggcgcg ctgatggggc tggccggcgc gcccctggcc
     3841 ctgtacatga gctacatcga gcccacctcg gcgttcagtc tgaactatgc ggtgtcggca
     3901 ctcgccatgc cgatcgtcgg cggcaccggc agttggctgg gcccggtgat cggcgcgctg
     3961 ctgctgggct ccacgcagca gatcgtcacg gtgaccgtgt cctcggagtt gaacgttctg
     4021 atcgtcggcc tgctgctggt ggccttcgtg gtggtggcac cgcagggcat cgtcggcctg
     4081 ttgcagcacc tgctgcggcc gcgaggcgca aagtgaccgc cgccctgctc gacgtgcagt
     4141 ctgtgtccaa gcgcttcggc ggcttcgccg ccctcacgga cgtgagcctc ggcgtgcgcg
     4201 gcggcgagcg cctcggcatc atcggaccga acggctcggg caagaccacg ctgatcaact
     4261 gcatctgcgg agcgctgcgc ggccatcgcg ggacgatcgt gttcgacggc cacgacgtca
     4321 ccgggctcgc gccgtaccag cgcacgcggc tgggcatcgc gcgcagcttc cagatcccgc
     4381 ggccgttcgc cagcatgagc ctggtggaca acgtcgcggt gccgctggaa tacgtggcgc
     4441 gccaggatct gacaaaccgg gatccggcac cccggcatgg gaagcgctgg ggaccactgg
     4501 ccgccgccga catccgcgcc gaagcgctgg cgatcctcga ccagatgggc ttgcagggcg
     4561 gcgcggacgc tcccgccgcc acactcaccc aggtcgggct gcgcaagctc gagctggcgc
     4621 gcgccatggc ggcgcgccca cggctgctga tcctggacga ggtgatggcc ggcctgtcct
     4681 cgaccgaggt ggacgaggtg ctgacgctgc tgttcacgct gagcgagcgc ggcgtgacca
     4741 tcctcatgat cgagcacatc atgcgcgcga tcatgcgctt ctcggagcgc gtggtatgcc
     4801 tggacgccgg gcgcgtgatg gccgaggggc cgccggcgac gatcgccgcc gatcccatgg
     4861 tgcgcaggat ctacctcggt gcttaagctc accgtgcagg acttggaggc cggctacggc
     4921 gccgtacgcg cgctgcacgg cgtgtcgctg gaggtacgcc agggcgagac ggtggcgctg
     4981 ctgggcacca acggcaacgg caagagcacg ctgatgaagt gcatcatggg cctgctgcgg
     5041 ccggcgcgcg gctccgtcgt gctggagctg gacggcaacc gcatcgacct caccggcaag
     5101 agcccggcac agatcgtcgg cctcggcgtg gcgatggtgc cggaggggcg ccggctgttc
     5161 ccgcgcctga ccgtgcagga gaatctgctg ctcggcgcgt tccggcaggc ggcgcgcccg
     5221 tcgattcgcg acaacctggc gctgtgctac gaggcgtttc cgatcctgcg cgaccggccg
     5281 gaccagctcg ccggcagcct gagcggcggc cagcagcaga tgctcgccat cgcccgcgcc
     5341 atcatgtcct cgccgaaatt gttgttggtc gacgagccgt cggtgggtct gtcgcccatg
     5401 ctggtgcgac agaccatcga cgtgatccgc gacctgaagc agcgcgccgg cctgacggtg
     5461 ctgatggccg agcagaattt ccacgaagcg agccgcatcg ccgatcgagg ctatatcatc
     5521 gtgcacggcg agatcgcctt cgaaggcagg gacctggaag agctgcagcg cagcgacctg
     5581 gtgaagagct attacctcgg acaatagagc actcgggaaa ggaaaaccaa cgatggcgac
     5641 gttgccgcag atcggctgga tcggcgccgg ccgcatgggc gtgccgatgg ccggattcct
     5701 gttgcaggcc ggctacccgg tgcgcgtgta ctcgcgcact gccgcgagcc gtcagaagct
     5761 cgtggcgcgc ggggcgacgg aggcgcaatc cgtggcggag tgcgcgcgcg gcgccgagat
     5821 cgtgttctgc tcggtgtcgg acgacgcggc gctgcgcgag gtcgcgctcg ggccgggcgg
     5881 cgtgctggcg agcctcgcgc ccggcgccgt cttcgccgag accagcacgg tatcggtgga
     5941 ggtgtccgag caggtcgcgc aggaagcggc gcggcgcggg gtcgcctact tgcggctgcc
     6001 gatctcgggc aatgccgcgc aggcgcagaa gggcgagctg acggtgctgg tctcgggccc
     6061 gctggacgcc tggaaccgga tcaagcccgt ttgcgagcgc ttcagcaagg cgcaggtcta
     6121 tctcggcgcg ggcgagcagg cgcgcgtcat gaagctggtg gtcaatgcgc tggtgtacgt
     6181 cttcgcgcag gcgatggccg aggcggtgac cctcggccgc aaggccgggc tggactgggg
     6241 cgtcatgctg gacgccctgg cgcagagcgc gctcgcctcg ccgtggctga aggtgaaggc
     6301 cgagctgatg aagcggcgcg acttcacccc gaccatgacg ccgccgctgg tgctgaagga
     6361 catcgatctg atgatggcct gcgcgcgggc gcagcaagtg tcgatgccgc tcaccgcgct
     6421 cacgcgccag ctgatgcaga cgctcgtcgg ccagggtttc ggcgacgagg actacatggc
     6481 ggcgatcaag ctcgccgaga agcaggccgg gctgccgagc gatcgcgtgg actgatcgag
     6541 agggcgaatg aggccagcga acccgggacg agccctcgtc atcggcggct ccatcgccgg
     6601 cctgatcgcc ggcaacctgc tgcatcgcgc gggctgggac gtgcacgtgt tcgagcgtgc
     6661 ctccggcgac ctcgagggcc gcggcgcggg catcaccatc ctgcccggcc tggtcagcag
     6721 tttccaggcc gccggcgtgg accagaccga gcgatcgctc ggcatcgagc tgcccgcccg
     6781 catcgcgttg gaccgcgctg gccgcgcgat cgtcgagcgc gtgttcgcgc agtcgatgac
     6841 ctcgtggggc cgtctgtatc aggcgctgag gaacgtctat ccggcggacc gttaccacgg
     6901 cggcatggcg ctcgagcgcg tggagcaaca cgcggatcgg gtgagcgcgg tgttcgccgg
     6961 cgggcagcgc gtggagagcg atctgctgat cggcgcggac ggcctgcgct cgacggtgcg
     7021 cagccagttc ctgccggagc tgcagcccta ttactcgggc tacctggcgt ggcgctgcct
     7081 ggccgacgag cgcgatttga cgccggaggt gttcgccccg ctgttcaacc gctacacggt
     7141 gtgcgtggcg cccggcgagc agggaatcgg ctatccggtg cccggcccgg atcacagcgt
     7201 ggcgcccggt cggcggcagt acaacgtggt ctggtatcac ccggtgcgcg aggccgagga
     7261 gctgccgcgt ttcatgaccg acgaccgcgg gcgctgccac cccaacggca ttccgccgtc
     7321 gctgctcagc agcgcgatcc gcgacgagat ggtgggcatc gcgccaggcg tgctcgcgcc
     7381 gcagttcgcc gaggcgctgc gccgcggcaa gctgcatttc tttcagccga tcctcgacat
     7441 cgagccgcag cggctcgcct tcggccgcgt tgcgatcatc ggcgacgccg cgttcgtcgc
     7501 gcgcccgcac gtggccatgg gcgtgcccaa gggcgccggc gacgcgctgg cgctggtgca
     7561 gtcgatcgct gccgccggcg gcgacgtgcc cgcgggtctg cagcgcttcg aggccgagcg
     7621 cctgcgggtg ggccgcacca tcgtcgcgcg cgggcgctac ctcggcgcct acatggaagc
     7681 gcagctcaag tcagaggccg agcgccgcgc ggccgaggcg cagcgcgagc cggagcgagt
     7741 gatgatggaa accgccgcgc cgctggatta cgacgcgatg gcgcagactg ctacggggtc
     7801 gacgggttga agtttttctt caggccctgc cagcactgat agtagtcggc ctgcagctgc
     7861 gtcgactgca gcgcgaacgc ggtcggccga atcacgcagc ggctctcgaa catgaacgcc
     7921 atcgtgtcgc ggatgacgtc gtgcttcgac aggtcggcgt tgctcgcttt ctcgaagatg
     7981 gcggcgtcgg gaccgtggcc gctcatgcag ttgtgcaggc tggccccgcc cggcacgaaa
     8041 ccctcggcct ttgcgtcgta cgcgccgtgg atcaggccca tgaactcgct ggcgacgttg
     8101 cggtggaacc acggcggccg gaaggtgtgc tgcatggcga ggatgcgtgg cgggaagatg
     8161 acgaagtcga tgctgtccca gccgggcgtg tcgctcaacg actgcagcac caggaagatc
     8221 gacggatccg gatggtcgta gctgatcgac ccgatgacgt tgaagcggcg cagatcgtac
     8281 ttgtatggcg cgtggttgcc gtgccaggcg accacgtcca gcggcgaatg gccgattccc
     8341 gccgcccaca ggttgcccat gaacttcgcc agcagctcga agccgccctc cttgtcctcg
     8401 taccacgcca cgggcgtttg gaagtcgcgc ggattggcca ggccgttcga tccgatcggc
     8461 cccaggtccg gcagacggaa ggcggcgccg aagttctcgc agatgtagcc gcgcgcggtc
     8521 gcgtccagca gctcgacgcg gaagcgcacc ccgcgcggga tgacgacgat ctcctgcggc
     8581 tcggcgtcga tcaggcccag ctcggtggcg aagcgcagcc gcccctgctg cggaacgatc
     8641 agcatctccc cgtcggcgtt gtagaagaaa cggccgctca tcgagcggtt ggccgcgtac
     8701 aggtgaatgc cgcagccgct ctgggcatgc ggatcgccgt tgcccgccat ggtcaccagc
     8761 ccgtcgacga agtcggtgga ggcattcggc atcggcagcg gatcccagcg cagctggttc
     8821 ggcggcgtgg cggtagaggc gaagttgctg gtgagcgtct cgtgatccac gcgccggaac
     8881 gggcgatgca ccgccgccgg ccggatccgg tacagccacg agcggcggtt cgcaccgcgc
     8941 ggcgccgtga acgcggtacc cgacaactgc tcggcataca ggccgtacgg gcacttctgc
     9001 ggggagttct gtcccaacgg cagcgcgccg ggcagcgctt cggtcgcgaa ctcgttggcg
     9061 aaacccgtct ggtagcgcag cccagagtct ttccgttgtg gggcgtcctt ctgttgtgcg
     9121 gcgtctttct gttgtccggt gctgccttgt tgctcggcca tcggctcagc cctccagcgc
     9181 ttgccacatt tccttgtccc gctcggcggt ccagatgcgc ggatcgcgat agc
//
LOCUS       DASPGE010000422         5650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1050613, whole genome shotgun sequence.
ACCESSION   DASPGE010000422 DASPGE010000000
VERSION     DASPGE010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050613"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VD791_13920"
     CDS             complement(<1..316)
                     /locus_tag="VD791_13920"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HXV09126.1"
                     /translation="MENESMDAAVLPVNPVVSAAVEAHLALLNAVRQNLLEDIETAAE
                     MLVRAAVARKRIFWAGNGGSAADSQHLAAELIGRFKRERRSIASVALTTDSSVLTSIG
                     NDY"
     gene            complement(335..1288)
                     /locus_tag="VD791_13925"
     CDS             complement(335..1288)
                     /locus_tag="VD791_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006759076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HXV09127.1"
                     /translation="MKASRARVLVTGGAGFLGSHLCGRLIERGHEVLCVDNFFTGTRG
                     NIAPLLGHPRFELMRHDVTFPLYVEVDEIYNLACPASPVHYQFDPVQTTKTSVHGAIN
                     MLGLAKRTKARILQASTSEVYGDPQVHPQTEDYWGHVNPVGLRSCYDEGKRCAETLFF
                     DYYRQHRLRIKVARIFNTYGPNMHPGDGRVVSNFIVQALRDGDLTVYGDGSQTRAFCY
                     VDDLIEGLIRLMETGDEFTGPVNLGNPGEFTIRELAQRVIELTGSRSKIVYQPLPADD
                     PVQRCPDIGLAERTLGWRPRVPLEEGLRRTIAYFRELLDLK"
     gene            1480..2433
                     /locus_tag="VD791_13930"
     CDS             1480..2433
                     /locus_tag="VD791_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV09128.1"
                     /translation="MPPIAAFAAAAVLLPLLLLPPARRWMLDRPNFRSLHAAPVPRTG
                     GLGLVPAAALGMALAGGHLLAAGLALGLMLLSVLDDWKGLSARVRLLAHLAIALAFVL
                     AVAGQSGWLVIAGLVLAVAWMTNLYNFMDGADGLAGGMAVFGFGAYAVAAWFGGDQPL
                     ALASLSVAAAAGAFLLFNFPPARIFMGDAGSIPFGFLAAALGVAGWREGLWPAWFPAA
                     VFASFVLDATVTLLRRALRGDGIWQAHRSHYYQRQILMGWTHRQLALFEYSLMAAAAL
                     AAVFALHLAPSAQAVVLGLLVGLHLAAGAAVDLRWRTRQEA"
     gene            2435..4312
                     /locus_tag="VD791_13935"
     CDS             2435..4312
                     /locus_tag="VD791_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014722573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HXV09129.1"
                     /translation="MPPFERFNPRTALAMVHDVAASALAWVAAFWLRLNLELPPGHAA
                     TMIQTLPLVVAVQALVFWRFGLYRGIWRYASLSDLRRLVIAVIVAALAAPFCLILLRL
                     SDPVPRSVLLLDPLLLLFIMGGSRLLYRGWKEHRLVRLSALNAEPVLVIGAGDTGATL
                     IRELGRGNEWRVIGLLDDNPTKQRRQIHGVPVLGRLEDVQRVTEHFALRAAIIAMPSA
                     PHVVRRRAALLCAQAGIKVMTVPSFDDLVSGKVQVSAIRRIELDDLLGRDPVALDQNI
                     LSGFLAGKTILVTGAGGSIGSELCRQIARFEPARLILFELSEFALYQIEQEFRANHTG
                     LDIVCAVGDARNHPRVEQILREYRPRVVFHAAAYKHVPLMEEVNAWEAVQNNVLGTHV
                     LASAAAQAGVEAFVMVSTDKAVNPVNVMGATKRLAEMVCQALQREIGTRYVMVRFGNV
                     LGSAGSVIPKFRQQIADGGPITVTHPDITRFFMSIPEAAQLVLQAGAMGHGGEIFVLD
                     MGEPVRIVDLARELIRLSGLREDDIEIVFSGLRPGEKLYEEVLADDERTLATPHPKLR
                     IARARGVAPDWLPRLMEWLGSAGACPDDQVRVGLSRFVPEYSAARDERPAQEVAKARY
                     G"
     gene            complement(4420..5358)
                     /gene="lipA"
                     /locus_tag="VD791_13940"
     CDS             complement(4420..5358)
                     /gene="lipA"
                     /locus_tag="VD791_13940"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HXV09130.1"
                     /translation="MSDPSNRQTGAAKTARNPVKIIPITPLKKPEWIRVKAGNSQTFY
                     EVKRKLRAHNLHTVCEEASCPNIGECFGKGTATFMILGDLCTRRCPFCDVAHGRPKPP
                     DPQEPTNLARTIADLGLRYVVITSVDRDDLRDGGAAHFAQCIRAVREHSPATRIEILT
                     PDFRSRLERALDALAQGLPDVMNHNLETVPRLYRMARPGADYAHSLKLLLDFKARFPG
                     IPTKSGIMVGLGETDEEILQVMRDLRRHGVDMLTIGQYLQPTSGHLPVLRYVEPARFK
                     EYEREALAMGFGHAACGPLVRSSYHADQQAHGVVAS"
     gene            complement(5355..>5650)
                     /locus_tag="VD791_13945"
     CDS             complement(5355..>5650)
                     /locus_tag="VD791_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase"
                     /protein_id="HXV09131.1"
                     /translation="LDLLQDYAVRGARREGAPGIYAGGAKVAALGLRIRGGCSYHGLS
                     LNVDLDLAPFAAIDPCGYPGLPVTRLRDLGIVESAAGVSERLAHHLIEQLSTP"
BASE COUNT          793 a         1888 c         1959 g         1010 t
ORIGIN      
        1 cgtagtcgtt gccgatcgag gtgaggaccg agctgtcggt ggtgagcgcc accgaagcga
       61 tcgaccggcg ctcgcgcttg aaccggccga tcagttcggc cgcgagatgc tgggaatcgg
      121 ctgcgctccc gccgttgcca gcccagaaga tccgtttgcg tgcgacagcc gcgcgcacca
      181 gcatctcggc ggcggtctcg atgtcctcga gcaggttctg ccgaacggcg ttcagcaggg
      241 cgagatgggc ctcgaccgct gcggagacga ccggattgac gggtagcacg gctgcatcca
      301 ttgattcgtt ctccatagtc ggcgcggttg ccgctcactt taggtccagc aactcgcgga
      361 agtacgcgat ggttcttctc agcccctctt cgagcggcac gcgcggacgc cagccgagcg
      421 tccgctcggc cagcccgatg tccgggcagc gctggaccgg gtcgtcggcg ggcagcggct
      481 gatacacgat cttcgaccgc gatccggtca actcgatcac ccgctgagcc agctcgcgga
      541 tggtgaattc cccggggttg cccaggttca ccgggccggt gaactcgtcg cccgtttcca
      601 tcagccggat caggccctcg atcagatcgt ccacatagca gaaagcgcgc gtctgcgacc
      661 catccccgta cacggtcagg tccccgtccc gcaaggcctg gacgatgaag ttggacacca
      721 cgcgcccgtc ccccggatgc atgttcggcc cgtaggtgtt gaaaatgcgc gctaccttga
      781 tgcgcaggcg gtgctggcgg taatagtcga agaacagcgt ctcggcgcag cgcttgcctt
      841 cgtcgtagca ggagcgcagc cccaccgggt tgacgtgtcc ccaatagtcc tcggtctgcg
      901 gatgcacctg cgggtcgccg tacacctcgc tggtcgaggc ctgcaggatc ctggccttgg
      961 tgcgcttggc caggcccagc atgttgatgg cgccgtgcac gctggtcttg gtggtttgca
     1021 ccggatcgaa ctggtagtgc accggggagg cggggcaggc caggttgtag atctcgtcca
     1081 cctccacgta cagcgggaag gtgacgtcgt ggcgcatcag ctcgaagcgt ggatggccga
     1141 gcagaggagc gatgttgccc cgggtcccgg tgaagaagtt gtccacgcag aggacctcgt
     1201 gtccgcgctc gatcagtcgc ccgcacaggt gcgagcccag gaacccggcg ccgccggtga
     1261 ccagtacgcg cgctcgagaa gccttcagat tgctgccctc cgcatgccgt gggctgttgg
     1321 caaatcgggc aatgctaaca tacgcgagga cgcggatgag ccgcccgctt ggataccgat
     1381 acaattgagc ccgctgcgcg cagagcgtgc gccccggcag cagcgccctg ccgccgggct
     1441 cccctcccca cctggagtcg acctctgctt tcgagcctcg tgccacccat agccgccttc
     1501 gcggcggccg ccgtgctgct gccgttgctc ctgctgccgc cggctcggcg ctggatgctc
     1561 gatcgcccga acttccggtc gctgcacgcc gcgccggtgc cacgcaccgg cggcctcggc
     1621 ctggtgcccg ccgcggcgct cggcatggca ctggctggtg gccacttgct cgcagcggga
     1681 ctggccctgg gactcatgct gctgtcggtg ctggacgact ggaaggggct ctccgcgcgc
     1741 gtgcgcctgc tggcgcacct cgccatcgcg ctcgccttcg tccttgcagt cgcggggcag
     1801 tcgggctggc ttgtgatcgc cgggctggtc cttgcggtgg cgtggatgac caacctgtac
     1861 aacttcatgg acggggccga tggcctcgct ggcgggatgg cggtgttcgg cttcggggcc
     1921 tatgccgtgg cggcctggtt cggcggcgat caaccgcttg cgctcgcgag cctgtcggtg
     1981 gccgccgccg ccggtgcgtt tctgctgttc aactttccgc cggcgcgcat cttcatgggc
     2041 gacgcgggct cgatcccgtt cggtttcctg gccgctgcgc tcggtgtagc cggctggcgc
     2101 gagggtctgt ggccggcgtg gtttccggcc gccgtgttcg catcctttgt gctggatgcg
     2161 acggtcacgc tgctgcgacg cgccctgcgg ggtgacggga tctggcaggc gcaccgcagc
     2221 cactactatc agcggcagat cttgatgggc tggacgcatc gccagctggc gctgttcgag
     2281 tacagcttga tggcggccgc cgcgctcgcc gcagtgtttg cgctgcatct tgcgccctcg
     2341 gcgcaggccg tagtgctcgg gctgctcgtc ggcctccatc tcgcggccgg cgccgctgta
     2401 gacctgcgct ggcgcactcg gcaggaggcc tgacatgccg cctttcgagc gcttcaaccc
     2461 ccgcaccgcg ctcgccatgg tgcacgacgt cgcggccagc gcgctggcct gggtggcggc
     2521 tttctggctg cgtttgaacc tcgagctacc cccgggccat gccgcgacca tgattcaaac
     2581 gctgccgctc gtggtcgcgg tgcaggcgct ggtgttctgg cgcttcgggc tgtaccgcgg
     2641 tatctggcgt tacgccagct tgagcgacct gcggcgcctg gtgatcgccg tgatcgtggc
     2701 cgccctggca gctcccttct gcctgatcct gctgcgcctg tccgacccgg tgccccgttc
     2761 cgtgctcctg ctcgatccgc tgttgttgct cttcatcatg ggcggcagcc gcctgctcta
     2821 ccgcgggtgg aaggagcacc gcctggtgcg cctgtccgcg ctgaatgcgg agccggtgct
     2881 ggtgatcggc gcgggagaca ccggggccac cctcatacgc gagctggggc gcggcaacga
     2941 atggcgggtg atcggcctgc tcgacgacaa tcccaccaag cagcgccgcc agatccacgg
     3001 cgttcccgtg ctcggccgtc tcgaggacgt gcagcgcgtg acggaacatt tcgcgctgcg
     3061 cgccgccatc atcgccatgc cttcggcgcc gcacgtggtg cgccggcgag ccgccctgct
     3121 gtgtgcccag gccgggatca aagtgatgac ggtgccgtcc ttcgacgatc tggtgagcgg
     3181 caaggtgcag gtgtcggcga tacgccgcat cgagctcgac gacctgctcg gccgcgatcc
     3241 cgtggcgctc gatcagaaca tcttgtccgg ttttctggcc ggtaagacga tcctggtaac
     3301 cggggccgga ggttccatcg gatcggaact atgccgccag atcgcccgct tcgagccggc
     3361 gcgcttgatc ctgttcgagc tgtccgagtt cgcgttgtat cagatcgagc aggagttccg
     3421 cgccaaccac accgggctgg acatcgtctg cgccgtgggc gatgcccgca accacccgcg
     3481 cgtggagcag atcctgcgcg aatatcggcc gcgggtggtg ttccatgcgg cggcgtacaa
     3541 gcacgtgccg ctgatggagg aggtcaacgc ctgggaagcg gtgcagaaca acgtgctcgg
     3601 cactcacgtg ctggcgtcgg ccgcggccca ggccggggtg gaggcgttcg tcatggtctc
     3661 caccgacaag gcggtcaatc ccgtcaacgt catgggcgcc accaagcgcc tggccgagat
     3721 ggtttgccag gcgctgcagc gcgagatcgg cacgcgttat gtgatggtgc ggttcggcaa
     3781 cgtgctgggc agcgcgggca gcgtcatccc gaagttccgc cagcagatcg ccgacggcgg
     3841 cccgatcacg gtcacgcatc ccgacatcac gcgcttcttc atgtccatcc ccgaagcggc
     3901 ccaactggtg ctgcaggccg gcgccatggg ccacggcggg gagatcttcg tgctcgacat
     3961 gggcgagccg gtgcgcatcg tggacctggc gcgcgagttg atccggctgt cgggtctgcg
     4021 cgaggacgac atcgaaatcg tgttcagcgg cctgcgcccg ggcgagaagc tgtacgagga
     4081 ggtgctggcc gacgacgaac gcacgctcgc cacgccgcat cccaagctgc gcatcgcgcg
     4141 cgcgcgtggc gttgcgcccg actggctgcc gcggttgatg gaatggctcg gcagcgccgg
     4201 cgcctgcccg gacgaccagg tgcgcgtggg gctgagccgg ttcgttcccg agtattcggc
     4261 cgcgcgcgac gagcggcctg cgcaggaggt ggcgaaggcc cgttacggtt gaccggccgg
     4321 atcgggagga agaatccgtc tgtccccgga tcccgccgcg ctggaccacc ccgccccttc
     4381 ggggcacccc tccttggaaa ggaggggaac tcaactacat cagcttgcga ccacgccgtg
     4441 cgcctgctgg tcggcgtgat agctcgagcg caccagcggc ccgcaggcgg cgtggccgaa
     4501 gcccatcgcg agcgcctcgc gttcgtactc cttgaagcgc gccggttcga cgtagcgcag
     4561 caccggtaga tgcccgctgg tcggctgcag atactggcct atggtgagca tgtccacgcc
     4621 gtggcggcgc agatcgcgca tcacctgcag gatctcttcg tcggtttcac ccaggcccac
     4681 catgatcccg gacttggtgg ggatgccggg gaagcgcgcc ttgaagtcca gcagcagctt
     4741 gagcgagtgc gcgtagtcgg cgccgggacg cgccatgcgg tacagccgcg ggaccgtttc
     4801 cagattgtgg ttcatcacgt ccggcaggcc ctgcgccagc gcgtcgagcg ccctttccag
     4861 ccggctgcgg aaatccggcg tgaggatctc gatccgagtc gccggggaat gttcgcgcac
     4921 ggcgcggatg cattgcgcga agtgcgccgc gccgccgtcg cgcaagtcgt cgcgatccac
     4981 gctggtgatg accacgtacc gcaagccgag atcggcgatg gtgcgggcca ggttcgtcgg
     5041 ctcctgcgga tccggcggct tgggacggcc gtgcgccacg tcgcagaacg ggcagcggcg
     5101 ggtgcacagg tcgcccagga tcatgaaagt ggcggtgcct ttgccgaagc attcgccgat
     5161 attggggcag gaagcctcct cgcacaccgt gtgcaggttg tgcgcgcgca gcttgcgctt
     5221 gacctcgtag aaggtctggc tgttgccggc cttcacccgg atccactccg gcttcttgag
     5281 cggtgtgatc ggaatgatct tgaccgggtt gcgcgccgtc ttcgctgctc cggtctggcg
     5341 gttgctgggg tcgctcatgg tgtggaaagc tgctcgatca ggtgatgcgc gaggcgctcg
     5401 ctcacgcctg cggcggattc gacgataccc aggtcgcgca gccgcgtgac cggcaggccg
     5461 gggtagccgc acgggtcgat ggccgcgaac ggggccaggt ccagatccac gttcaggctc
     5521 agaccgtggt agctgcaccc gccgcggatg cgcagaccca gggcggccac cttcgcgccg
     5581 cctgcataga tacccggggc gccctcgcgg cgtgctccgc gcacagcgta gtcttgcagc
     5641 aagtctagca
//
LOCUS       DASPGE010000423         7993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1519983, whole genome shotgun sequence.
ACCESSION   DASPGE010000423 DASPGE010000000
VERSION     DASPGE010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519983"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VD791_13950"
     CDS             <1..649
                     /locus_tag="VD791_13950"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXV09132.1"
                     /translation="GWKDGDYFYNGVRSQDVGTARGFRKMGTEKAGGFFTRGILIDIA
                     ALKGVQRLQKGYAITMADYRAALQRQGISDATQGDVVLFRTGWNSLWKTNHLGRPPGE
                     KVKTDEQMMKDTAEFGSGEPGPAPEVCDHLASKKISMVGSDTWGLEPYDFAKGMPESF
                     AYCHMNLIVRRGISNFENLDLDDLSADKAYEFLFAWAPLKLVGATGSPGNPVAAW"
     gene            776..1378
                     /locus_tag="VD791_13955"
     CDS             776..1378
                     /locus_tag="VD791_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HXV09133.1"
                     /translation="MTKIIDYYFSPMSPWTYLGHERFAALARKHGAAIDVRPVDYGKI
                     FPASGGLPLAKRAPQRQKYRLAELKRWREYLGLPLTIEPKYFPYDTALAARTILVAKR
                     SGGEAAAMRLTGALLRATWVEERNMADTAQLAAVLREQGLDAAALLQSAGTPQIDAEY
                     QRLTQEAIERDVFGAPTYAYNGELFWGQDRLDLLDRALAR"
     gene            complement(1407..2615)
                     /locus_tag="VD791_13960"
     CDS             complement(1407..2615)
                     /locus_tag="VD791_13960"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HXV09134.1"
                     /translation="MSVTLQDILAAAGALGGNVVHTPCLHSRTLSAVTGAQIYLKFEN
                     HQFTASFKERGALNKLLSLTPAQRARGVVAASAGNHAQGVAYHARRLGIPAVIVMPRY
                     TPGVKVVHTRGHGAEVVLHGEVFDEAKAQARALAEERGLTMVHPYDDEKVIAGQGSIA
                     LEMLGAYPDLDALVVPIGGGGMISGMAVAAKGLNPQVEVIGVQTRRFPSMYCAIKGAT
                     PEFGVTTIADGIAVKEPGALTVPLVRALVSDILLVDEGDVEQAVVLLLEVEKTVVEGA
                     GAVGLAAVLAHRARFEGRKVGLVLSGGNIDPLMLADIIERGMVRTGRLTRLSVELRDL
                     PGALAKVTACLAEQNANIEEIFHQRAFTNLPVQTVEVDFVLETRDREHVQQVIEALGA
                     AGFTARLHND"
     gene            complement(2713..2916)
                     /locus_tag="VD791_13965"
     CDS             complement(2713..2916)
                     /locus_tag="VD791_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HXV09135.1"
                     /translation="MATGTVKWFNESKGYGFITPDEKGEDLFAHFSAIKMDGFKTLKE
                     GQRVSFEVTTGPKGKQASNIQGI"
     gene            3253..4077
                     /locus_tag="VD791_13970"
     CDS             3253..4077
                     /locus_tag="VD791_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXV09136.1"
                     /translation="MLTRTIPGSGEALPLIGLGTYRAFDAGASAAERTALAEVLKTLV
                     ARGGRMVDSSPMYGRAEGVVGDLQAQLGLRPQLFLATKVWTSGRDAGIRQMQRSLQSM
                     QAGTMDLMQIHNLVDVHTHTATLEEWKRQGTVRYLGITHYHEGAHSELERLIATRRYD
                     FVQLNYCMAERTAERRLLPLARETGVAVIVNRPFARASLLSRVRGKALPPWAAEFDCS
                     SWSQFFLKYVVSHPAVTCAIPATSEPAHMLNNAMAGDGRLPDELMRVRMVRYFESL"
     gene            4214..6061
                     /locus_tag="VD791_13975"
     CDS             4214..6061
                     /locus_tag="VD791_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HXV09137.1"
                     /translation="MTLLQRITRGIAAAAMAAAGAASAAMVPGAGPAQWAGDLTPISA
                     RDWSYARAAHLLERAGFGGTPEEIAALAAMTPEQAVRRLVQHQEVDNGALPPFDHSDV
                     WDAGLDPFPVSRPATTDQARAKGVALGVEVKPAGNRRMQPVVNKFFYWLRASRLETNR
                     AAYWWGNRMLVTKRPLQEKMALFWHGHFATSEEKVRDYRKMLVQLELFQARGTGNFRD
                     LLIGVAQDPAMLQFLDAGVNVKGAPNENFAREIMELFTMGVGNYSEQDIREAARAFTG
                     WNFRDLQFVVNREQHDDGDKTVLGQRGAFDGVKVIDVILAQPVTAEFIAGKIYRFFVR
                     EELGPDLRRRLGAVLRDNRYEIAPLLTAIFLSRDFYSAPSIGSRIKGPVELVVSTYKK
                     LGVNELPGVPDFNDTTAALGQQLFRPPTVAGWAQGRSWVTPGLLIERGNFARDVLFPD
                     ITYLPTDRYPSLITGDEIRAVHEKISMGYDITTATKPVGMTGQTEGMSMSNAMADRDE
                     DFNTRYASYRGWQMAVERVKPIPRTYVRLDLAGVVEARGLTDTTQVVDYFLARFLRVP
                     LDAERRARLIAFLDQELGTSEVARADTYMEDALRMLVHLIMSMPEYQLG"
     gene            6116..7360
                     /locus_tag="VD791_13980"
     CDS             6116..7360
                     /locus_tag="VD791_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HXV09138.1"
                     /translation="MTRFEIERLLARRKLLKAGLAGVCTHGAGALGLPLIFGRAAAAM
                     AAAVTGDNRILVVMELSGGNDGLNTLVPYADDAYYRHRPGIGIKPNKLRKIDQHYGFS
                     GGMAGFERLYKDGKLAVVHGCGYDQPSFSHFTSMAYWHTAAPNSGGEYGWVGRLADGM
                     KPGGAPNYLVNIDETQSLAVRSREHVPVVFDDPEKFVRKAFYQERPLFEQMAHAGDFA
                     NPAQRYLADVARSAQEASALVRQAWSRYQTPVDYGIFPVDLKKVAALIDAGLPTRLYY
                     VAYRHNAFDTHVHQSDLHARQLTYVSDAVLGFMRDLERIGRAGDVTLLIFSEFGRRVP
                     ENTSLGTDHGTANCMFLVGQRVKGGHYGTPVSLTNLDEGDNLIHTCDFRRVYATAIEG
                     WMGYASSAALLGGRFEPFPVFG"
     gene            complement(7374..>7993)
                     /locus_tag="VD791_13985"
     CDS             complement(7374..>7993)
                     /locus_tag="VD791_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXV09139.1"
                     /translation="AQPMSAVGTGADIGILERSSWNNPEPEAVLAVNRAGKVVGATLG
                     NDVNLRDWEGRSALLLGRAKDNNGSCAIGPFIRLLDGKFGADELRRLRIEVQVSGNDG
                     FEVKGGYSLTQISRDPLDLAAQAVNRNHQYPDGLVLFLGTAFAPTQDRGEAGRGFTHR
                     LGDVVTIRSPSLGTLSNRIGYCDRIAPWTFGLSDLMRNLAARGLI"
BASE COUNT         1336 a         2731 c         2708 g         1218 t
ORIGIN      
        1 cggctggaag gatggcgatt acttctacaa cggcgtgcgc tcgcaggatg tggggaccgc
       61 gcgcggcttc cgcaagatgg gcacggagaa ggcgggcggt ttcttcaccc gcgggatcct
      121 gatcgacatc gccgcgctga agggcgtgca gcggttgcag aagggttacg ccatcaccat
      181 ggcagactac cgcgctgcgc tacagcgtca gggcatcagc gacgccacgc agggcgacgt
      241 ggtgctgttc cgcaccggat ggaacagtct gtggaagacc aaccacctgg gccggcctcc
      301 gggcgagaag gtgaagaccg acgagcagat gatgaaggac acggccgagt tcggctcggg
      361 tgagccgggc ccggcaccgg aggtgtgcga ccacctggcg tccaagaaga tctccatggt
      421 cggttccgac acctggggct tggagccgta cgacttcgcc aagggcatgc cggaatcctt
      481 cgcctactgc cacatgaacc tgatcgtgcg gcgcggcatc agcaacttcg agaacctgga
      541 tctggacgat ctgtccgcgg acaaggccta cgagttcctg ttcgcgtggg cgcccctgaa
      601 gctggtgggc gccaccggct cgccggggaa cccggtcgcc gcctggtaac acccatgtgc
      661 gcctctcccc cgcccgggga gaggtggcgt tggcttcgag cgccggcgcg cgaatgcgcc
      721 ggcgcttgtt tcttgcgcat cgcgcgcctg gatcggacac acgcaggctg catccatgac
      781 caagatcatc gactactact tctccccgat gtcgccctgg acctacctgg ggcacgagcg
      841 cttcgccgca ctggcgcgca agcacggtgc tgcgatcgac gtcaggccgg tcgactacgg
      901 caagattttt ccggcgtcgg gtgggctgcc gctcgcgaag cgcgcgccgc agcgccagaa
      961 gtaccggctg gcggagctca agcgctggcg cgaatacctc gggctgccgc tcaccatcga
     1021 gccgaaatac tttccctacg acaccgcgct cgccgcgcgc acgatcctgg tcgccaagag
     1081 gagcggtggc gaggccgcgg cgatgcggct tacgggcgcg ctgctgcgcg cgacctgggt
     1141 ggaagagcgc aacatggcgg acacggcgca gctcgcggcc gtcctgcgcg agcagggcct
     1201 cgacgccgcc gcgttgctgc aatcggcggg aaccccgcag atcgacgccg agtaccagcg
     1261 cctgacccag gaggcgatcg agcgcgacgt gttcggcgcc ccgacctacg catacaacgg
     1321 cgagctgttc tgggggcagg accggctcga tctgctggac cgcgccctgg cgcgctgaag
     1381 cctcgcgccg atgtgagaaa tgcgggctag tcgttgtgca ggcgcgcggt gaacccggcc
     1441 gcgcccagcg cctcgatcac ctgctgcacg tgctcgcggt cgcgcgtctc gagcacgaag
     1501 tccacctcca ccgtctgcac cggcaaattg gtgaaagcac gctggtggaa gatctcctcg
     1561 atgttggcgt tctgctccgc caggcaggca gtcaccttgg cgagcgcgcc gggcaggtcg
     1621 cgcagctcga cgctgagccg ggtgagccgc ccggtgcgca ccatgccgcg ctcgatgatg
     1681 tcggccagca tcaacgggtc gatgttgccg cccgacagca ccagccccac cttcctgcct
     1741 tcgaagcggg cgcgatgcgc cagcaccgcg gcaagcccga cggcgcctgc gccttcgacc
     1801 acggtttttt cgacctcgag cagcagcacc accgcctgct cgacgtcgcc ctcgtccacc
     1861 agcaggatgt cgctcaccag cgcgcgcacc agcggaacgg tgagcgctcc gggctccttc
     1921 acggcgatgc cgtcggcgat ggtggtgacg ccgaactccg gcgtcgcgcc cttgatcgcg
     1981 cagtacatgg aagggaagcg ccgcgtctgc acaccgatca cctcgacctg cggattgagc
     2041 cccttggccg cgaccgccat ccccgagatc atgccgccgc ccccgatcgg caccaccagg
     2101 gcgtccaggt cgggatatgc ccccagcatc tcgagcgcga tgctgccctg ccccgcgatc
     2161 accttctcgt cgtcgtacgg gtgcaccatg gtcaggccgc gctcctcggc cagcgcccgc
     2221 gcctgcgcct tcgcctcgtc gaacacctcg ccgtgcagca ccacttcggc gccgtgcccg
     2281 cgcgtgtgca ccaccttgac gcccggggtg tagcgcggca tgacgatgac cgcggggatg
     2341 ccgagccggc gcgcgtgata ggccacaccc tgggcgtgat taccggccga ggcggcgacc
     2401 acgccgcggg cgcgctgcgc gggcgtgagc gacaacagct tgttgagcgc gccgcgctcc
     2461 ttgaagctgg cggtgaactg atggttctcg aacttgaggt agatctgcgc cccggtgacg
     2521 gcggacagcg tgcgcgagtg caggcagggc gtgtgcacca cgttgccgcc gagcgcgccg
     2581 gcggcggcga ggatgtcctg cagggtgacg ctcatgcccg gtagcccgag gtagaaaagt
     2641 taggcgcaga aaagtcgagc atagaaaaga aaaaacccgc cgcagcgggt tttttcatcg
     2701 gctcggtcgg gctcagatgc cctggatatt ggaggcctgc ttgcccttgg gcccggtggt
     2761 gacctcgaag ctcacccgct ggccctcctt gagcgtcttg aagccgtcca tcttgatcgc
     2821 ggaaaaatgc gcgaacagat cctcgccttt ctcgtccggg gtgatgaaac cataaccctt
     2881 ggactcgttg aaccacttga ccgtaccagt tgccataaaa aataccctca gaaaacgatg
     2941 gagacagcgg gctggcgccc gtactgatgt ttgggctgca agcccgcctg cggcaatgcc
     3001 gatacggggt gagacttgac ccgaaacatg cgggcggatg atacgccttt cccgggaaaa
     3061 tgccagagcc gatcaggctc tggggtcggc ccttcgccat gacgccgggg tagcgcatga
     3121 aacgcatgtc cccatgccga ccgattacaa tgcgccgctg cctcatgctt ccgccccggg
     3181 ccgcagcgcc gtgctgctgc gccgcatcgc agggcggcct cgtccgcagc atgacggcag
     3241 gcaagtgaac gcatgctgac gcgcacgatc cccggttccg gcgaggccct gccgctgatc
     3301 gggctgggca cttaccgggc cttcgacgcc ggcgcctcgg cggcggagcg cacggcgctc
     3361 gccgaggtgc tgaagacgct ggtcgcgcgc ggcggcagga tggtagacag ctcgcccatg
     3421 tacggccgcg ccgaaggcgt ggtgggagac ctgcaggcgc agctcggcct gcgcccgcaa
     3481 ctgttcctgg cgaccaaggt gtggaccagc gggcgcgatg ccggcatccg ccagatgcag
     3541 cgctcgctgc agtcgatgca ggccggcacc atggacctga tgcagatcca caacctggtg
     3601 gacgtgcaca cgcacaccgc cacgctggag gagtggaagc ggcaaggcac ggtgcgctac
     3661 cttggcatca cgcactacca cgagggcgcc cacagcgagc tggagcggtt gatcgccacg
     3721 cgccgctacg acttcgtgca gctcaattac tgcatggccg agcgcaccgc ggagcggcgc
     3781 ctgctgccgc tcgcgcgcga gaccggtgtg gcggtgatcg tcaatcgccc gttcgcacgg
     3841 gcgagtctgc tctcgcgcgt gcgcggcaag gcgctcccgc cctgggcggc cgagttcgac
     3901 tgcagcagct ggagccagtt ctttctcaag tacgtcgtct cgcacccggc ggtgacctgc
     3961 gcgattcccg ccacgagcga gcccgcgcac atgctgaaca acgcgatggc cggcgatggg
     4021 cgcctgcccg acgagctcat gcgtgtgcgc atggttcgct atttcgagag tctgtgaacg
     4081 cggccgcggg atggcgcgat gccggcccgc cgcttcgcgg gccacgcgaa aaatcatgat
     4141 aatcatgagc cccgggcagc ggccggggcc ggcacaatgg ccaccatcag gcccgcgcgc
     4201 aaggagcaag gcgatgacgt tactgcagcg cattaccaga gggatcgcag ccgcagccat
     4261 ggcggccgcg ggcgcggcga gcgcagcgat ggttcccggc gcgggcccgg cgcagtgggc
     4321 cggcgacctc accccgatct cggcccgtga ctggagttac gcgcgtgccg cacacttgct
     4381 ggaacgcgcg ggcttcggcg gcacgccgga ggagatcgcg gccctggcgg cgatgacgcc
     4441 cgagcaggcc gtgcgccggc tggtgcaaca ccaggaagtc gacaacggcg ctcttccgcc
     4501 cttcgaccac tcggacgtgt gggacgcggg gctcgatccc ttcccggtga gccgtccggc
     4561 caccaccgac caggccaggg caaagggcgt tgccctcggc gtggaggtga agccggccgg
     4621 caaccgcagg atgcagccgg tggtgaacaa gttcttctac tggctgcggg cgagccgtct
     4681 ggagaccaat cgcgccgcct actggtgggg caaccgcatg ctcgtcacca agcggccgct
     4741 gcaggagaag atggcgctgt tctggcatgg ccacttcgcc accagcgaag agaaagtgcg
     4801 cgactaccgc aagatgctgg tgcagctcga gctgttccag gcgcgcggca ccggcaactt
     4861 ccgcgacctg ctgatcgggg tggcgcagga cccggcgatg ctgcagttcc tggatgccgg
     4921 ggtcaacgtc aagggcgcgc ccaacgagaa cttcgcccgc gagatcatgg agctgttcac
     4981 catgggcgtg ggcaactaca gcgagcagga catccgcgag gcggcgcgcg cgttcaccgg
     5041 ctggaatttc cgcgatctgc agttcgtggt caaccgcgag cagcacgacg acggcgacaa
     5101 gaccgtgctc ggccagcgcg gcgccttcga cggcgtcaag gtgatcgacg tgatcctggc
     5161 gcagccggtc accgccgagt tcatcgccgg caagatctac cgcttcttcg tgcgcgagga
     5221 gctcggcccg gatttgcgcc gccgcctggg ggcggtgctg cgcgacaacc gctacgagat
     5281 cgctccgctg ctcaccgcca ttttcctgtc gcgcgacttc tacagcgccc ccagcatcgg
     5341 cagccgcatc aaaggaccgg tggagctggt ggtgtccact tacaagaagc tcggcgtgaa
     5401 cgagctgccg ggtgtgcccg acttcaacga caccaccgcc gcgctcggcc agcagctgtt
     5461 ccgcccgccc accgtggccg gctgggcgca gggccgcagc tgggtcacgc ccgggctgct
     5521 gatcgagcgc ggcaacttcg cgcgcgacgt gctgttcccg gacatcacct acctgcccac
     5581 cgaccgctat ccgtcgctca tcaccgggga cgagatccgc gcggtacacg agaagatctc
     5641 catgggctac gacatcacca ccgccaccaa gccggtcggc atgaccgggc agaccgaggg
     5701 catgtcgatg tccaacgcca tggccgaccg ggacgaggac ttcaacacgc gctacgcgag
     5761 ctaccgcggc tggcagatgg cggtggagag ggtcaagccg atcccgcgca cctacgtacg
     5821 gctcgacctc gccggcgtgg tggaggcgcg cggcctgacc gacaccacgc aggtggtgga
     5881 ctacttcctc gcgcgcttcc tgcgggtgcc gctcgacgcc gagcggcgcg cgcgcctgat
     5941 cgccttcctc gaccaggagc tgggcaccag cgaggtggcg cgcgccgata cttacatgga
     6001 ggatgccctg cgcatgctcg tgcacctgat catgagcatg ccggagtacc agctcggctg
     6061 acagccttcg gacgcaacac cccggacggc ggcacccgcg aaaggatgaa cgaacatgac
     6121 gcgctttgag atagagagac tgctcgcccg gcgcaagctg ctgaaggcgg gcctggccgg
     6181 agtgtgcaca cacggcgcgg gcgcattggg actgccgctc atcttcggac gcgcggcggc
     6241 cgccatggcg gccgccgtaa ccggcgacaa ccgcatcctg gtggtgatgg agctgtccgg
     6301 cggcaacgac ggcctcaaca ccctggtgcc ctacgccgac gacgcctatt accggcaccg
     6361 ccccggcatc ggcatcaagc cgaacaagct gcgcaagatc gaccagcatt acggcttcag
     6421 cggcgggatg gccgggttcg agcgtctgta caaggacggc aagctggcgg tggtgcacgg
     6481 ctgcggctac gaccagccct cgttctcgca cttcacttcg atggcctact ggcacaccgc
     6541 cgcgcccaac agcggcggcg agtacggctg ggtcgggcgc ctggcggacg gcatgaagcc
     6601 gggcggcgcg cccaactacc tggtcaacat cgacgagacg cagtcgctcg cggtgcgcag
     6661 ccgcgagcac gtgccggtgg tgttcgacga cccggagaag ttcgtgagga aggctttcta
     6721 ccaggaacgg ccgctgttcg agcagatggc gcacgcgggc gacttcgcca acccggcgca
     6781 gcgctacctg gcggacgtgg cacgcagcgc gcaggaggcc tccgccctgg tgcgccaggc
     6841 ctggtcgcgc taccagacgc cggtggatta cggcatcttc ccggtggacc tgaagaaggt
     6901 ggcggcgctg atcgacgccg gcctgcccac gcggctgtac tacgtggcct atcggcacaa
     6961 cgccttcgac acgcacgtgc accagagcga tctgcacgcg cgccagctga cctacgtgtc
     7021 cgatgccgtg ctcggcttca tgcgcgacct ggagcgcatc ggccgcgccg gcgacgtgac
     7081 gctcctgatc ttctccgagt tcggccgccg cgtgccggag aacaccagcc tgggcaccga
     7141 tcacggcacg gcgaactgca tgttcctggt gggccagcgg gtcaaaggcg gccactacgg
     7201 cacgccggtc agcctgacca atctcgacga aggcgacaat ctgatccaca cttgcgattt
     7261 ccggcgcgtg tatgcgaccg ccatcgaagg ctggatgggg tacgccagca gcgctgcgct
     7321 gctcggaggc aggttcgaac cgtttccggt tttcgggtaa accccgacgc gactcagatc
     7381 aggccgcgcg ccgcgaggtt gcgcatcagg tcactgaggc cgaaggtcca gggcgcgatg
     7441 cgatcgcaat agccgatgcg gttggacagc gtgccgagcg agggcgaacg gatggtcacc
     7501 acgtcgccca gccggtgggt gaaaccgcgc cccgcctcgc cgcggtcctg cgtgggcgcg
     7561 aacgccgtgc ccaggaacag caccagaccg tccggatact ggtgattgcg gttcaccgcc
     7621 tgggccgcta ggtcgagcgg gtcgcgactg atctgcgtca ggctgtagcc gcccttcacc
     7681 tcgaagccgt cgttgccgct cacctgcacc tcgatgcgca gccggcgcag ctcgtccgcg
     7741 ccgaacttgc cgtccagcag gcggatgaag ggaccgatgg cgcagcttcc gttgttgtcc
     7801 ttcgcgcgtc cgagcagcag cgcgctgcgg ccttcccagt cgcgcaggtt gacgtcgtta
     7861 cccagcgtcg caccgaccac tttgccggcg cggttgacgg ccagcaccgc ctcgggctcg
     7921 gggttgttcc acgaggagcg ctcgaggatg ccgatgtcgg cgccggtgcc caccgccgac
     7981 atcggctggg ctt
//
LOCUS       DASPGE010000424         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1638957, whole genome shotgun sequence.
ACCESSION   DASPGE010000424 DASPGE010000000
VERSION     DASPGE010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638957"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VD791_13990"
     CDS             complement(<1..388)
                     /locus_tag="VD791_13990"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV09140.1"
                     /translation="MKRLAALALAVLAGGAAAQAVQVAPGTVDELRAIYATSLEIAEG
                     ERIAKSNCARCHGASGISATKGVPHLADQRAAYLDHQLRQYRFGARPKSAMTGAVRDL
                     SPEVLVNVAAYYASLPPPRPAPPPAKG"
     gene            complement(393..683)
                     /locus_tag="VD791_13995"
     CDS             complement(393..683)
                     /locus_tag="VD791_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C'"
                     /protein_id="HXV09141.1"
                     /translation="MKTAVWMVAVAGVLAAGASLPAAAADELKAKGCLNCHEMDKKKV
                     GPAFKDVAAKHKGNAGAEAMLVEKLKTGKGHMKIKGTDAEIQAAVRQALEAK"
     gene            complement(713..>2599)
                     /locus_tag="VD791_14000"
     CDS             complement(713..>2599)
                     /locus_tag="VD791_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HXV09142.1"
                     /translation="PAKPAAAKAAEPVALDNETCLSCHGNEGFEMPGPDGKPRNLHVV
                     QKKFAMSVHGKRQCVECHRDITAVPHEKTAPLKVSCVQCHEDLWRAMQEKRPGAEQNP
                     RLGVVADMIERYMKSVHARPRRDDQSRTNATCYHCHEAHYVYPKDSEARQEWRLSIPD
                     RCGACHTKQRAEYATSVHGKEVLENKNLYAAVCSDCHSTHDIDRPEGDPIKLAITANC
                     GTCHAESLRTYTQTYHGKVSTLGYAHAAKCFDCHGGHTVLRVADPASKVHPDNRLDTC
                     RKCHTGATAGFLTFEPHATTDDYARYPYVWIAYRFMIALLIGVFLFFWTHTALWFYRE
                     YRDRKEGKTRPHVQVDESMQLKSKQYQRFGPVWRLAHLVFAVSVMTLVLTGMTVFFAD
                     TNWAKHVAALFGGPKVTAIVHRTAAAIMLGIFAVHLVYLLGRIGGNLRTFRWFGPTSL
                     VPTWQDLKDIGAMFAWFFGRRPRPAFDRWTYWEKFDYWAVFWGMAIIGGSGAMLAFPV
                     AVASVLPGWVFNVATIVHGEEAVLAAVFLFTVHFFNNHFRPDKFPLDIVMFTGAVPLE
                     EFRREHALEYQRLAASGELQKHLVDAPSRAMTLGSRILGFTLIAIGLLLLLLVLAGFA
                     RRMG"
BASE COUNT          367 a          888 c          895 g          449 t
ORIGIN      
        1 cgcccttggc gggcggtggc gcgggccgcg gcggcggcag gctcgcgtag taggcagcca
       61 cgttgacgag cacttccggg ctcagatcgc gcactgcgcc ggtcatcgcg ctcttcggcc
      121 gcgcaccgaa gcggtactgg cgcagctgat ggtcgagata cgccgcgcgc tggtcggcga
      181 ggtgcggcac gcccttggtt gcgctgatgc cgctggcgcc atggcagcgc gcgcagttgg
      241 acttcgcgat gcgttcgcct tccgcgatct cgagcgaggt ggcgtagatc gcgcgcagct
      301 cgtccaccgt gcccggcgcc acctgcacgg cctgggcggc ggcgccgccc gcgagtacgg
      361 cgagcgccag tgccgcgagg cgcttcacca ggctacttcg cttccagcgc ctggcgcacg
      421 gcggcctgga tctcggcgtc cgtgcccttg atcttcatgt ggcccttgcc cgtcttgagc
      481 ttctccacca gcatggcctc ggcgcccgcg ttgcccttgt gcttggccgc tacgtccttg
      541 aacgcgggac cgaccttctt cttgtccatc tcgtggcagt tcaggcaacc cttcgccttg
      601 agctcgtctg ccgccgctgc cggcaggctc gcgcctgcgg cgagcacgcc cgccaccgcc
      661 accatccaca cggccgtctt catgtcgcct cctatgtaga cctgcattgc gttcacccca
      721 tgcgcctcgc gaagccggcc agcacgagga gcaggagcaa caggccgatc gcgatcagcg
      781 tgaagccgag gatcctggag cccagggtca tcgcgcgcga gggcgcgtcc accaggtgct
      841 tttgcagctc ccccgaggcc gcgaggcgct gatactcgag cgcgtgctcg cgccggaatt
      901 cctccagcgg caccgcgccc gtgaacatga cgatgtcgag cgggaacttg tcgggccgga
      961 agtggttgtt gaagaagtgc acggtgaaca ggaacacggc ggcgagcacc gcttcctcgc
     1021 cgtgcacgat cgtggccacg ttgaacaccc acccgggcag gaccgacgcg accgctaccg
     1081 ggaaggcgag catcgcgccg ctgccgccga tgatcgccat gccccagaac accgcccagt
     1141 agtcgaactt ctcccagtag gtccagcggt cgaacgccgg ccgcggccgc cgcccgaaga
     1201 accaggcgaa catcgcgccg atgtccttga ggtcctgcca ggtgggtacg agcgaagtgg
     1261 ggccgaacca ccggaacgtc ctcaggttgc cgccgatgcg cccgagcagg tagaccaggt
     1321 ggacggcgaa gatgccgagc atgatcgccg cggccgtgcg gtgcacgatg gccgtgacct
     1381 tggggccgcc gaacagggcg gccacgtgct ttgcccagtt ggtgtcggcg aagaataccg
     1441 tcatgccggt gagcaccagc gtcatgacgc taaccgcgaa cacgaggtgg gcgaggcgcc
     1501 acaccggccc gaagcgctgg tactgcttgc tcttgagctg catggactcg tccacctgca
     1561 cgtgcgggcg cgtcttgcct tccttgcggt cgcggtactc gcggtagaac cacagcgcgg
     1621 tgtgcgtcca gaaaaacagg aacacgccga tcagcagggc gatcatgaac cggtacgcga
     1681 tccacacgta cgggtagcgc gcgtagtcgt ccgtggtggc gtgcggctcg aacgtcagga
     1741 agcctgcggt cgcgcccgta tggcacttgc ggcaggtgtc gaggcgattg tccggatgca
     1801 ccttcgaggc cgggtcggcc acgcgcagga cggtgtggcc gccgtggcag tcgaagcact
     1861 tggcggcgtg ggcgtagccc agcgtgctga ccttgccgtg gtaggtctgg gtgtaggtgc
     1921 gcaggctctc ggcgtggcag gtgccgcagt tggcggtgat cgccagcttg atcgggtccc
     1981 cctccggccg gtcgatgtcg tgcgtcgagt ggcagtccga gcacaccgcg gcgtacaggt
     2041 tcttgttctc cagcacttcc ttgccgtgca ccgaggtggc gtactcggcc cgctgcttcg
     2101 tgtggcacgc gccgcagcgg tccgggatgc tcaggcgcca ctcctggcgc gcctcgctgt
     2161 ccttcggata cacgtaatgc gcctcgtggc agtgatagca ggtcgcgttg gtgcgcgact
     2221 ggtcgtcgcg ccgcggccgc gcgtgcaccg acttcatgta gcgctcgatc atgtcggcca
     2281 cgacccccag gcgcgggttc tgctccgcgc cgggccgctt ctcctgcatc gcgcgccaca
     2341 ggtcctcgtg gcactgcacg cagctcacct tgagcggcgc ggtcttctcg tgcgggaccg
     2401 ccgtgatgtc gcggtggcac tcgacgcact gccgcttgcc gtgcacgctc atcgcgaact
     2461 tcttctgcac gacgtgcagg ttgcgcggct tcccgtccgg gccgggcatc tcgaaacctt
     2521 cgttgccgtg gcagctgagg caggtctcgt tgtcgagcgc gaccggttcc gcggccttgg
     2581 ccgcggcggg cttggcggg
//
LOCUS       DASPGE010000425         4386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_936532, whole genome shotgun sequence.
ACCESSION   DASPGE010000425 DASPGE010000000
VERSION     DASPGE010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936532"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VD791_14005"
     CDS             <1..579
                     /locus_tag="VD791_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXV09143.1"
                     /translation="KERNGMTQEAWRRQHELFERAFWALGDLPLPAIAAVNGPAYAGG
                     LELALMCDFIYAARSARFALTEVSLGIMPGAGGTQNLPRAVGERRAKEIILTARPFGA
                     QEAHRWGLVNRLCEPGELLAAALDTAAAIARNAPLAVRQAKKSVRYGMQMDIGSAYRF
                     EVEAYHRLVDTEDRREGIRAFNEKRKPDFKGS"
     gene            complement(581..1795)
                     /locus_tag="VD791_14010"
     CDS             complement(581..1795)
                     /locus_tag="VD791_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV09144.1"
                     /translation="MVGNVLEWYDFAVYGYLATIIARRFFPASDELTGLLSAFAAFGV
                     GFVVRPLGGVVIGRLADTRGRKTALVLTIVLMALGTVGIGLIPDYHSIGVLAPALLVA
                     CRLLQGFSAGGEWGGATAFIVEWAPAQRRGFFGSFQQASVAAGFLLGSGSAALVSTLL
                     SAEQMDVWGWRLPFLIGVLLLPVGIYMRRNVEETPAYRAVQTRTTPVQAPAGWRLAAK
                     AFGFTVLWTVAYYIVLTYMPTFTQKHAGLTPTQALWSNTLCLLVLVLAIPVIGHLSDR
                     VGRKPLLLACCAGFVLLPWPLFGAMVQSGTFATVLLVQIVFALTVALFSGPGPAAIAE
                     MFPTESRTTWMSTGYSLAVAVFGGFAPYVATWLIGATGSPLSPTFYLIGAALVSTAVI
                     FALPETAHDALR"
     gene            complement(1976..2572)
                     /locus_tag="VD791_14015"
     CDS             complement(1976..2572)
                     /locus_tag="VD791_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXV09145.1"
                     /translation="MVTDPRYALDRRQAVAALLDARDDALVVSGLGSTTYDVHAAGDH
                     PGNFYLWGAMGGAAMIGLGLALAQPARRVLVITGDGEQLMGLGALATIGAVAKPANLG
                     IAVIDNGSYAETGMQRSHTGYGADLCAIGRGCGFQKTFEAADLAAVAQLRDLFQRGGG
                     PILGVVRVSAHNPPRSLPSRDAVHLKNRFRAHLGLPQQ"
     gene            complement(2566..3129)
                     /locus_tag="VD791_14020"
     CDS             complement(2566..3129)
                     /locus_tag="VD791_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HXV09146.1"
                     /translation="MTAASPPATATAASSSIPAGRDWPAEIHRVLKEARVQQVSYVPD
                     AGHARLIALCADDPGVRTVPLTTEEEGVALAAGAWLGGQRAALLLQSSGVGNCINMLS
                     LASIARFPLLMLVTMRGEWSEFNPWQVPMSRATQPSLEAIGVRVYRGDTVEEVAETVA
                     AAADLAFDGDQQVAVLLSQRMLGKKIW"
     gene            3259..4215
                     /locus_tag="VD791_14025"
     CDS             3259..4215
                     /locus_tag="VD791_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV09147.1"
                     /translation="MSATKKFASQADLAEKKISFDKLSEHAYAYTAEGDPNTGIVVGD
                     DAVMVIDTQATPVMAQDVIRRIREVTDKPIEYVMLSHYHAVRVLGASGYEPRHIIASR
                     DTYDLIVERGEQDMKSEIERFPRLFRAVESVPGLTWPTLAFEKRMTLWLGKLQVEMMQ
                     LGRGHTKGDTVVWLPQEKILFSGDLVEFDATPYTGDAYLGDWPATLDAIAALQPAKLV
                     PGRGASLQTPAQVKAGLDATRAFVVEMFESVKRGAAAGKDLRTVYRETYGLLKPKFGH
                     WVIFDHCLPFDVSRAYDEATRHRDPRIWTAERDKEMWQALEG"
BASE COUNT          759 a         1508 c         1532 g          587 t
ORIGIN      
        1 aaggagcgca acggcatgac gcaggaggcg tggcgccggc agcacgagct gttcgagcgc
       61 gccttctggg cgctggggga tctgccgctg ccggcgatcg ccgcggtcaa cggcccggcc
      121 tatgccggcg ggctggagct cgcgctgatg tgcgacttca tctacgcggc gcgctcggcg
      181 cgcttcgcgc tcaccgaggt gtcgctcggc atcatgcccg gtgcgggcgg cacgcagaac
      241 ctgccgcgcg ccgtgggcga gcggcgcgcc aaggagatca tcctcaccgc gcgcccgttc
      301 ggcgctcagg aggcgcaccg atgggggctg gtgaaccgcc tgtgcgagcc gggcgagttg
      361 ctggccgcgg cgctggacac ggccgctgcg atcgcccgca atgcgccgct cgccgtgcga
      421 caggcgaaga aatcggtgcg ctacggcatg caaatggaca tcggcagcgc ctatcgcttc
      481 gaggtggaag cctaccaccg gctggtcgac accgaggacc ggcgcgaggg catccgcgcc
      541 ttcaacgaga agcgcaaacc cgatttcaag gggtcttgac ctagcgcagc gcgtcgtgcg
      601 ccgtctccgg cagcgcgaag atcacggcag tcgacaccag cgctgcgccg atcagataga
      661 aggtcggcga cagcggcgag ccggtcgcgc cgatcagcca ggtggccacg tacggcgcga
      721 agcctccgaa caccgcgacc gccaggctgt agccggtgga catccaggtg gtgcgcgatt
      781 cggtcgggaa catctccgcg atggcggccg gcccgggtcc ggagaacagc gccacagtca
      841 gcgcgaagac gatctgcacc agcagcaccg tggcgaaggt gccgctttgc accatggcgc
      901 cgaacagcgg ccagggcagc aaaacgaacc cggcgcagca ggcgagcagc aggggtttac
      961 ggccgacacg gtcggacaga tgcccgatca ccgggatcgc cagcaccagg accagcaggc
     1021 acagcgtgtt cgaccacaac gcctgcgttg gcgtcagccc cgcgtgcttc tgcgtgaacg
     1081 tgggcatgta ggtgaggacg atgtaatagg cgaccgtcca cagcacggta aacccgaacg
     1141 ccttggcggc gagccgccag cctgctggcg cctggaccgg cgtggtccgc gtctggaccg
     1201 cccggtatgc cggcgtctct tccacgttgc ggcgcatata gatgcccacc ggcagcagca
     1261 gcacaccgat caggaacggc aggcgccagc cccagacgtc catctgctcg gcgctgagca
     1321 gcgtgctcac cagcgcggcg ctgcccgaac cgagcaggaa tccggcggcc acgctcgcct
     1381 gctgaaagct gccgaagaag ccgcggcgct gcgccggcgc ccattcgacg atgaaggcgg
     1441 tggccccgcc ccattcgccc cccgcggaga agccttgcag caggcggcac gccaccagca
     1501 gcgccggcgc caggacgccg atgctgtggt aatcgggaat caggccgatg ccgaccgtgc
     1561 ccagcgccat cagcacgatg gtcagcacca gcgcggtctt ccggccccga gtgtcggcca
     1621 ggcggccgat cacaacgccg cccagcgggc gcaccacgaa gcccacgccg aacgcggcga
     1681 aggccgacag caggccggtc agttcgtcgc tcgccggaaa gaagcgccgg gcgatgatcg
     1741 tcgcgaggta accgtagacc gcgaagtcgt accactcgag gacgttgccg accaccgcgg
     1801 ccgcgatggc gcggtgggtg ctggttccgg catccgagcg tgaattgctt cgatacgctt
     1861 gccgtaccac cccgtcacgc ttcgcgtgcc acccctcctc ggggaggagg ggagtttgct
     1921 ccccgcctct cacgaggtgg ggtgccggcg tagccggcgg ggcggtgtgg ggtgctcact
     1981 gctgcggcaa gcccaggtgc gcccggaagc ggttcttcag gtgcaccgcg tcgcgcgagg
     2041 gcagggagcg cggcgggttg tgcgcgctga cgcgcaccac gcccaggatc ggcccgccgc
     2101 cgcgctggaa caggtcgcgc agttgcgcga ccgcggcgag atccgcggcc tcgaaggttt
     2161 tctggaagcc gcaaccgcgt ccaatagcgc acaggtccgc gccgtaaccg gtatggctgc
     2221 gctgcatgcc ggtttcggcg tagctgccgt tgtcgatcac tgcgatcccc agattggccg
     2281 gcttcgccac cgcgccgatg gtggccagcg cgcccagtcc catgagctgc tcgccgtcgc
     2341 cggtgatgac cagcacgcgc cgcgccggtt gcgccagcgc cagccccagt ccgatcatgg
     2401 ccgcaccgcc catggcgccc cacagataga agttgccggg gtgatcgccg gcggcgtgca
     2461 cgtcgtaagt ggtggagccc aggccgctca ccaccagcgc atcgtcgcgc gcgtcgagca
     2521 gggcggcgac cgcctggcgg cggtcgagcg cgtagcgcgg atcggtcacc atatcttctt
     2581 gcccagcatg cgctgcgaga gcagaacggc cacctgctgg tcgccgtcga aagcgagatc
     2641 ggccgcggcg gccaccgtct cggccacttc ctcaaccgtg tcgccgcgat agacccgcac
     2701 gccgatcgcc tccaggctcg gttgcgtcgc acggctcatc ggcacctgcc agggattgaa
     2761 ctcgctccac tcgccgcgca tggtgaccag catcagcaac gggaagcgtg cgatcgacgc
     2821 cagcgacagc atgttgatgc agttgcccac gccgctcgac tgcagcagca gcgccgcacg
     2881 ctgacccccc agccacgcgc cggcggccag cgccacaccc tcctcctcgg tggtgagcgg
     2941 gaccgtgcgt acgccgggat cgtcggcgca caaagcgatc agccgggcgt ggccggcgtc
     3001 gggcacgtag gaaacctgct gcacgcgcgc ttccttgagg acgcggtgga tctcggccgg
     3061 ccagtcgcgc ccagcgggga tcgaggagga ggcggcggtg gccgtggcgg gcggggacgc
     3121 tgctgtcatg gcttctcgga cgaaaatcgg gtggcgcaag gctaccacgg cgccggcggc
     3181 cccgccgcgg cgcacgcggc gcctgcttga gcgacaatag cgggcgaccg agaacgaaac
     3241 gtccagaagg agcagcgcat gagcgcgacc aagaagttcg cctcccaggc cgacctggcg
     3301 gagaagaaga tcagcttcga caagctgagc gagcacgcct acgcctacac cgcggagggc
     3361 gatccgaaca ccggcatcgt cgtgggcgac gacgcggtga tggtgatcga cacccaggcc
     3421 accccggtga tggcgcagga cgtgatccgt cgcatccgcg aggtgaccga caagccgatc
     3481 gagtacgtga tgctgtcgca ttatcacgcg gtgcgcgtgc tgggcgcgtc cggctacgag
     3541 ccgcggcaca tcatcgccag ccgcgacacc tatgatctga tcgtggagcg cggcgagcag
     3601 gacatgaaga gcgagatcga gcgcttcccg cgcctgtttc gcgcggtcga gtcggtgccc
     3661 gggctgacct ggccgacgct cgcgttcgag aagcgcatga ccttgtggtt gggcaagctg
     3721 caggtcgaga tgatgcagct cgggcgcggc cacaccaagg gcgatacggt ggtgtggctg
     3781 ccgcaggaga agatcctgtt ctcgggcgac ctggtcgagt tcgacgccac gccttacacc
     3841 ggcgacgctt acctcggcga ctggcccgcc accctggacg ccatcgccgc gctgcagccc
     3901 gccaagctgg taccggggcg cggcgcgtcg ctgcagactc cggcgcaggt gaaagccggg
     3961 ctggacgcca cgcgcgcgtt cgtcgtcgaa atgttcgagt cggtgaagcg cggcgccgcc
     4021 gcgggcaagg atctgcgcac cgtgtaccgg gaaacctacg gcctgctcaa gccgaagttc
     4081 gggcactggg tgatcttcga ccactgcctg ccgttcgacg tctcgcgcgc ttacgacgag
     4141 gccacgcgcc atcgcgatcc gcgcatctgg accgccgagc gggacaagga aatgtggcag
     4201 gcgctggagg gctgaaccga tggccgagca acaaggcagc gccccacaac agaaagacgc
     4261 cgcacaacag aaggacgccg cacaacggaa agacgcccca caacagaaag actctgggag
     4321 gctgcgctac cagacgggct tcgccaacga gttcgcgacc gaagcgctgt ccggcgcgct
     4381 accgtt
//
LOCUS       DASPGE010000426         5434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1288001, whole genome shotgun sequence.
ACCESSION   DASPGE010000426 DASPGE010000000
VERSION     DASPGE010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288001"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VD791_14030"
     CDS             <1..207
                     /locus_tag="VD791_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HXV09148.1"
                     /translation="PHDRIAALNTLQERAAAGEVVTGLLYVDADPDDLHGNLNTVDAP
                     FNQLAERELCPGAAALQKFNASLR"
     gene            295..789
                     /locus_tag="VD791_14035"
     CDS             295..789
                     /locus_tag="VD791_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV09149.1"
                     /translation="MSGRTTATFALDARADRWALPDGSIQRFRSGLAYRPLVLDLHPE
                     RNGVRLTLALDMPAAGSPAVDRYQATLFELDHPLLQQELSMNVRPGASASARIGTVQV
                     HTPGEHLFLLEELGAPALTPLRATLTVREEVERPLMPLVWAGVAMLLAGVGWMTYRLL
                     LRAG"
     gene            844..1302
                     /locus_tag="VD791_14040"
     CDS             844..1302
                     /locus_tag="VD791_14040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXV09150.1"
                     /translation="MRTSPVHPTSRCCAVVATFVALLCAPPPAGAVSELISALGSSEP
                     PFVPLSEEEAHHALERDLLLKVGKYLQVSDYPGEARRWRWTGTVLVEVLVAADGMVKQ
                     VALSRTSGFRVLDMQALEVVRRVSKLFVPVGLRQRDRTVTVPVSFHLQKS"
     gene            1314..1631
                     /locus_tag="VD791_14045"
     CDS             1314..1631
                     /locus_tag="VD791_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV09151.1"
                     /translation="MHLISLGNIVRAALAGVLLAAGVAIAQEPEFEGHCAMSAALGNK
                     LPTSCSVVWISPQTNKLYCFSSEAAKAAFIRDPQANEKSAQAFWKDPSFWEKLQKERG
                     DGG"
     gene            1719..2279
                     /locus_tag="VD791_14050"
     CDS             1719..2279
                     /locus_tag="VD791_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV09152.1"
                     /translation="MDYRRLEAGDLPAVLALQEANLLVNLTPEQRGQGFLSARFSPAQ
                     FTEMAAAVAVIVATDADGIAGYLCASSLAFNRRVPLLAAMIERFPDMRFLGRALSAQR
                     CLIYGPVCVAGSRRGGGVLRGLYAALRAEIAGDYDAGVLFIAKDNPRSFAAHANGLGM
                     TPVGDFAFADGHYWILAFAVPTSAQR"
     gene            complement(2301..2840)
                     /locus_tag="VD791_14055"
     CDS             complement(2301..2840)
                     /locus_tag="VD791_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010109938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXV09153.1"
                     /translation="MLNEQLDAIDWNILVRLQSDARISNVELARQVGLSPSPCLNRVR
                     ALEVSGYISRYVTLLDAQRVGLKVSVFIQVTLEKQIEPVLERFEAAIRDRPEVMECYL
                     MTGEADYLLRVVVPDLQALERFILNFLSRVPGVGNIKSSFALKQVKYQTALPLPAAEA
                     PRRIRRAAPRPGPRARRQR"
     gene            3006..>5434
                     /gene="aceE"
                     /locus_tag="VD791_14060"
     CDS             3006..>5434
                     /gene="aceE"
                     /locus_tag="VD791_14060"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006749406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HXV09154.1"
                     /translation="MTDLSDIISSGHWAVLPPDLDPVETREWLDALEAIVEAEGPERA
                     TFILRKLLDRARALRVPLPPVLNTPYCNTIPLSDQPQYPGNMEIESRISGLVRWNALA
                     LVVRANKKASELGGHIATYASVADLFEVGCNHFFRAGHSGDLIYWQPHAAPGVYARVY
                     LEGRLSEDQLDNYRQETGGPGLSSYCHPWLMPALWQFPTGSMGLGPITAIYQARFMRY
                     LENRGVLETRGRKVWAFVGDGEMDEPESIAGLSLAAREGLDNLIFVVNCNLQRLDGPV
                     RGNGSIIQELEGLFAGAGWNVIKVLWGSDWDPLFARDHENIILRRLHETVDGEFQKYA
                     ATDGRFNRENFFNKYPELQKLVAHLSDEDIDRLKRGGHDPVKIYAAYHAAVHHEGQPT
                     VILAQTKKGYGMGHWGQGRMTAHQQKKLEDEALLAFRDRFALPLSDEDVHHLRFHKPA
                     AESPEMKYLHQRRQALGGYIPERIGQARTLPAPALESMSKYLEGTGGREQSTTMVFVQ
                     LLAQWLRDAGMGKRIVPIVADEARTFGMQALFRQVAIYSSVGQVYEPEDKDELLYYKE
                     AKEGQILEEGITEAGAISSWIAAATSYSAHGVPMLPFYIFYSCFGFQRIGDLVWAAAD
                     SRSRGFLLGATAGRTTLSGEGLQHEDGSSHLLFSTVPNCVAYDPCFGYELTVILQDGM
                     RRMLERQEDVFYYVTVMNENYAHPPMPEGAREGIIKGMYRLREAREERARSEPGPRVQ
                     LLGSGAILREVLAAADLLQQDWNVAADVWSVTSFTELRREGMDVDRWNRLHPDAETRR
                     THVEKCLARSE"
BASE COUNT          878 a         1854 c         1826 g          876 t
ORIGIN      
        1 ccgcacgacc gcatcgccgc gctcaacacc ttgcaggagc gcgctgcggc cggcgaagtg
       61 gtgaccgggt tgctgtacgt cgatgcagat cccgacgatc tgcacggcaa cctgaacacc
      121 gtggacgcgc ccttcaatca gctggcggag cgggagctct gccccggcgc ggcggcgctg
      181 cagaagttca acgcctcgct gcgctgaacc cgtcccagcc tgaaccccgc cggcgtcctg
      241 ctgatcctgc tcggcctgct gctcggtccg ggctactacc tgtcttgcga acgcttgagc
      301 gggcgcacca cggcgacgtt cgcgctcgat gcgcgcgccg accgctgggc cctgcccgac
      361 ggcagcatcc agcgtttccg cagcgggctc gcctatcgac cgctggtcct cgacctgcac
      421 cccgagcgca acggcgtgcg gctgaccctg gccttggaca tgcccgccgc gggaagtccc
      481 gcggtcgatc gctaccaggc aacgctgttc gagctcgacc acccgttgct gcagcaagag
      541 ctgagcatga acgtgcggcc gggggcctcg gcatcggcga ggatcggcac ggtgcaagtc
      601 cacacgcccg gcgagcatct gttcctgctc gaggaactgg gcgcgccggc gctgacgccg
      661 ttgcgggcga ctctgacggt gcgcgaggag gtcgagcgac ccttgatgcc gctggtctgg
      721 gccggcgtgg ccatgctcct ggccggggtg ggttggatga cctaccggct gttgctgcgt
      781 gcgggctagc tcgcggcgtg gcccgggtct tgcagcgcgt gagctatact gctcccggcc
      841 aacatgagaa cctctcccgt gcacccgact tcacgatgct gcgctgtggt cgcgaccttc
      901 gtcgcgttgt tgtgtgcgcc accgcccgcc ggcgcggttt ccgaactgat atccgcgctc
      961 ggttcgagcg aaccgccctt cgtgccgctc agcgaggagg aagcgcatca tgctctggag
     1021 cgcgacctgc tgctcaaggt cggcaagtac ctgcaggtat ccgactatcc cggcgaggcg
     1081 cggcgctggc gctggaccgg caccgtgctg gtggaagtgc tggtcgccgc cgacgggatg
     1141 gtgaaacagg tcgcgctgtc gcgcaccagc gggttccggg tgctcgacat gcaggcgctc
     1201 gaggtggtgc gccgggtgtc gaagctgttc gtgccggtag gcctgcgcca gcgtgaccgc
     1261 acggtaacgg tacccgtcag cttccatctg cagaagtcgt gaggagacgc cgcttgcatc
     1321 ttatttcact tgggaatatc gtgcgcgccg cgctcgccgg cgtgctgctc gccgccggcg
     1381 tcgcgatcgc tcaggagccc gagttcgagg gccactgcgc catgagcgcc gccctgggca
     1441 acaagcttcc caccagctgc agcgtggtgt ggatctcgcc gcagaccaac aaactgtact
     1501 gcttcagcag cgaagcggcc aaagccgcct tcatccgcga cccgcaggcg aacgagaaga
     1561 gcgcccaggc gttctggaag gaccccagct tctgggagaa gctgcagaag gagcgcggcg
     1621 acggaggctg acgcgcgtcg gcgcgaagtg cccggactgc cgtgaacccc tcacccccgc
     1681 ccctctccca caaggggaga ggcgagttca atgccgggat ggactatcgc cgcctcgaag
     1741 cgggcgacct cccggcggtg ctcgcgctgc aggaagcgaa tctgctcgtc aacctgacgc
     1801 cggagcagcg cggtcagggc tttctgtcgg cgcgcttcag ccccgcgcag ttcaccgaaa
     1861 tggccgccgc cgtggcggtc attgtcgcca cggacgcgga tggaattgcg ggctatctgt
     1921 gtgcctccag cctggcgttc aaccgccgtg tgccgctgct ggcggcgatg atcgagcgct
     1981 tccccgatat gcggttcctt ggccgtgcgc tcagcgcgca gcgctgcctg atctacgggc
     2041 cggtgtgcgt ggccggctca cgccgcggtg gcggcgtgct gcgcggcctg tacgccgcgt
     2101 tgcgcgccga gatcgcgggc gattacgacg ccggcgtgct gttcatcgcc aaggacaacc
     2161 cgcgctcgtt cgccgcgcac gcgaacgggc tggggatgac gccggtgggc gatttcgcct
     2221 tcgcggacgg gcactactgg atcctcgcct tcgccgtgcc gacgtccgcg cagcgctgag
     2281 caggcccagg ttattggtga ctacctctgg cgccgcgccc gcgggcccgg gcgcggagcc
     2341 gcgcgcctga tgcgccgcgg ggcttcggcc gccggcaacg gcagcgcggt ctgatacttc
     2401 acctgcttga gcgcgaagct ggacttgatg ttgcccacgc ccggcacgcg cgacaggaaa
     2461 ttgaggatga agcgctccag cgcctgcaga tcgggcacca ccacgcgcag caggtagtcg
     2521 gcctcgccgg tcatcagata acactccatc acctcggggc ggtcgcggat cgccgcttcg
     2581 aagcgctcca gcaccggctc gatctgcttc tccagcgtca cctggatgaa cacgctcact
     2641 ttcaggccga cgcgctgcgc atcgagcaga gtcacatagc ggctgatgta gccgctcacc
     2701 tccagcgcgc gcacgcggtt cagacagggc gaaggcgaca gcccgacctg ccgcgccagc
     2761 tccacgttgg agatgcgggc gtcggattgg agccggacca ggatattcca atcgatcgcg
     2821 tcgagctgct cgttcagcat attctgctgt ctcacatcgg gtcttcagca ttatgcacca
     2881 tccacggcgg atttcaccag cgcagacgca accacatgct ttgcggtttg cagtacgatg
     2941 aggcaatcgc aatgctcgtc tccgagcacc cggcttcgag ctcctagctc ctggatcctt
     3001 cacagatgac cgacctctcc gacatcatct ccagcggcca ctgggccgtg ctgccacccg
     3061 acctcgatcc ggtcgagacg cgcgaatggc tcgacgcgct cgaggccatc gtcgaagccg
     3121 aagggccgga gcgcgccacc ttcatcctgc gcaagctcct ggaccgcgcg cgcgcgctgc
     3181 gcgtgccgct gcccccggtg ctgaacacgc cctattgcaa caccattccg ctgtccgacc
     3241 agccgcaata cccgggcaac atggagatcg agagccgcat cagcggcctg gtgcgctgga
     3301 acgcgctggc gctggtggtg cgcgccaaca agaaggcctc ggagctgggc gggcacatcg
     3361 ccacctatgc ttcggtggcc gatctgttcg aagtcggctg caaccacttc tttcgcgccg
     3421 gacacagcgg cgacctgatc tactggcagc cgcacgcagc gccgggcgtg tacgcgcggg
     3481 tctacctgga gggccgcctt tccgaagatc agctcgacaa ctaccggcag gagaccggtg
     3541 gccctgggct gtcctcctac tgccatccat ggctgatgcc ggcgctctgg cagttcccca
     3601 ccggttccat gggcctgggc ccgatcacgg cgatctatca ggcgcgcttc atgcgctatc
     3661 tggagaaccg cggcgtcctg gaaacgcgcg ggcgcaaggt gtgggcgttc gtcggcgacg
     3721 gcgagatgga cgagccggag tcgatcgccg ggctgtcgct ggccgcgcgc gaggggctgg
     3781 acaacctgat cttcgtggtc aactgcaacc tgcagcggct ggacgggccg gtgcgcggca
     3841 acggctcgat catccaggag ctggaggggc tgttcgcggg cgccggctgg aacgtgatca
     3901 aggtgctgtg gggatcggac tgggacccgc tgttcgcgcg cgaccacgag aacatcatct
     3961 tgcgccggct gcacgagacc gtggacggcg agttccagaa gtacgccgcc accgacgggc
     4021 gcttcaaccg cgagaacttc ttcaacaagt accccgagct gcagaagctg gtcgcgcacc
     4081 tgtccgacga ggacatcgac cggctcaagc gcggcggcca cgatccggtg aagatctacg
     4141 ccgcctacca cgccgcggtc caccacgagg gccagcccac ggtcatcctg gcacagacca
     4201 agaagggcta cggcatgggc cactggggcc aggggcggat gaccgcacac cagcagaaga
     4261 agctggagga cgaggcgctg ctggccttcc gcgaccgctt cgccctgccg ctgtcggacg
     4321 aggacgtgca ccacctgcgc ttccacaagc cggccgcaga gagccccgag atgaagtacc
     4381 tgcatcagcg ccgtcaggcg ttgggcggtt acattcccga gcgcatcggc caggcgcgga
     4441 cgctgccggc gcccgccctg gagagcatga gcaagtacct ggagggcacc ggcgggcgcg
     4501 aacagtccac caccatggtg ttcgtgcagc tgctggcgca gtggctgcgc gacgccggca
     4561 tgggcaagcg catcgtgccg atcgtcgccg acgaggcgcg caccttcggc atgcaggcgc
     4621 tgttccgtca agtggcgatc tattcctccg tcgggcaggt gtacgagccc gaggacaagg
     4681 acgagctgct ctactacaag gaggccaagg aaggccagat cctggaggaa ggcatcaccg
     4741 aggcgggagc gatctcatcc tggatcgccg cggccaccag ctacagcgcg cacggcgtgc
     4801 cgatgctgcc cttctacatc ttctactctt gcttcggctt ccagcgcatc ggcgacctgg
     4861 tgtgggccgc cgccgattcg cgcagccggg gcttcctgct cggcgccacc gcggggcgca
     4921 ccacgctgtc cggcgaaggc ctgcagcacg aggacggctc cagccacctg ctgttctcca
     4981 ccgtccccaa ctgcgtggcc tacgatccgt gcttcggtta cgagttgacg gtgatcctgc
     5041 aggacggcat gcgccgcatg ctggagcggc aggaggacgt gttctactac gtcacggtga
     5101 tgaacgagaa ctacgcgcat ccgccgatgc ccgagggcgc gcgcgagggc atcatcaaag
     5161 gcatgtatcg gctgcgcgag gcgcgagagg aacgcgcgcg gagtgagcct ggaccgcgcg
     5221 tccagcttct tggttccggc gccatcctgc gcgaagtgct ggccgccgcc gatctgctcc
     5281 agcaggactg gaacgtcgcc gccgacgtgt ggagcgtcac cagcttcacc gagctgcggc
     5341 gcgagggcat ggacgtggac cgctggaacc ggctgcaccc ggacgccgag acgcgccgca
     5401 cacacgtgga gaagtgcctc gcgagatcgg aaga
//
LOCUS       DASPGE010000427         4489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U16443
            k141_1403951, whole genome shotgun sequence.
ACCESSION   DASPGE010000427 DASPGE010000000
VERSION     DASPGE010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935438
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:14:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,818
            CDSs (total)                      :: 2,796
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403951"
                     /isolate="SMAG_U16443"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /gene="ccoN"
                     /locus_tag="VD791_14065"
     CDS             <1..1127
                     /gene="ccoN"
                     /locus_tag="VD791_14065"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HXV09155.1"
                     /translation="WQAVIVLAAITLPLGITSSKEYAELEWPIDILIAVVWVAYAIVF
                     FGTIWKRKVPHIYVANWFFGAFILTVALLHIVNSAAMPATFWKSYSAYAGATDAMVQW
                     WYGHNAVGFFLTAGFLGMMYYFIPKQAGRPVYSYRLSVVHFWALIFTYMWAGPHHLHY
                     TALPEWAQSLGMVFSLILLAPSWGGMINGIMTLSGAWHKLRTDPILKFLIVSLSFYGM
                     STFEGPMMSIKTVNSLSHYTDWTIGHVHSGALGWVGLVSMGSIYYLIPRLFGRSEMYS
                     VPLINVHFWVSTVGIVLYISAMWIAGVMQGLMWRAVSEDGTLTYTFVESVKATYPFYA
                     IRLLGGLLYLGGMLIMAWNVAKTIGAGRSVEAPIPAPAYA"
     gene            1138..1758
                     /gene="ccoO"
                     /locus_tag="VD791_14070"
     CDS             1138..1758
                     /gene="ccoO"
                     /locus_tag="VD791_14070"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HXV09156.1"
                     /translation="MKLTHDMIERNQALLIILVILVVAVGGLVEIVPLYFQRSVTEPV
                     AGLKPYGALQLTGRDVYVREGCYNCHSQMVRPFRAEVERYGRYSVAGEFVYDRPFQWG
                     SKRTGPDLHRVGGRYSDEWHRLHLMNPRDLVPESNMPAYPWLALTAANAGDIGAKMRA
                     LRTLGVPYADDEIAKAPEELKGKTEMEALIAYLQVLGTAGRAGAGK"
     gene            1767..1910
                     /locus_tag="VD791_14075"
     CDS             1767..1910
                     /locus_tag="VD791_14075"
                     /inference="COORDINATES: protein motif:HMM:NF017366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HXV09157.1"
                     /translation="MDLNLIRSLVTVAALAAFLGIVWWAYSPSRKKRLEDAGRSVLEE
                     NDQ"
     gene            1907..2812
                     /gene="ccoP"
                     /locus_tag="VD791_14080"
     CDS             1907..2812
                     /gene="ccoP"
                     /locus_tag="VD791_14080"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HXV09158.1"
                     /translation="MSEFTSSFWDYYISIITLVSIVACGVLLVAQSKRRVAGGTAETT
                     GHTWDEDLGEYNNPLPRWWMWLFWITIVFGFAYLALYPGLGSYPGMLKWTQVGQLERE
                     TARHEERFGPLYERYAKTDVAALAKDPAALAIGQKLFLNNCAQCHASDGAGSRGFPNL
                     TDRDWQWGGSAEAVKTTITQGRMGVMPPFGPALGEQGAKDAAHYVMALSGLAHDSIRA
                     ARGKALFAQNCVACHGAEGKGNPALGAPNLTDRTWLYSAAEPVIIETITRGRTNQMPA
                     HQDLLGEARIHLLTAYVLSLSQSAK"
     gene            2809..4188
                     /gene="ccoG"
                     /locus_tag="VD791_14085"
     CDS             2809..4188
                     /gene="ccoG"
                     /locus_tag="VD791_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HXV09159.1"
                     /translation="MKATSVPVQAVEQALYEVRKTIHPRAVHGWFAGWRWALVWATQL
                     VFYGLAWLPWNERQAVLFDIAHRKFYIFGLVFFPQDIIYLTVLLIISALSLFLFTAVA
                     GRLWCGYACPQTVYTEIFLWVEEKIEGDRPARLKLDAAPLSPRKFALKAAKHGAWIAI
                     ALWTGFTFVGYFTPIRELWDAVWSFSTGPWETFWVLFYGFATYGNAGWMREQVCKYMC
                     PYARFQSAMFDKDTLIITYDPERGEPRGKGPGKGHCVDCSICVQVCPTGIDIRDGLQY
                     ECIGCAACIDGCNRVMDKVGFPQGLIRYSTQHAMEEKLTRAQMFRRAFRPRVLVYSAI
                     LWAVIIGAAVFLWNRVPLKVDVIRDRAAISREVEGGQIENVYRLQIMNTAEGARAFRL
                     SVSGLPGIRIPGEAVVEIDGAASRMVPVKVQIAPGAAGAGTHRIEFAVQSTDEASVAV
                     REKSVFIVR"
     gene            4193..>4489
                     /locus_tag="VD791_14090"
     CDS             4193..>4489
                     /locus_tag="VD791_14090"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HXV09160.1"
                     /translation="MTPWYRERWTWIVMAPPAAAVLAGLATWWIAASGADGLVAEDYY
                     KQGLAINKVIAREERARALGISARLDIADGRIRVRLEGAAPEALFVHLAHRTRAG"
BASE COUNT          679 a         1526 c         1545 g          739 t
ORIGIN      
        1 ggtggcaggc ggtgatcgtg ctcgccgcga tcacgctgcc cctgggcatc accagctcca
       61 aggaatacgc cgagctcgag tggccgatcg acatcctgat cgcggtggtg tgggtggcct
      121 acgcgatcgt cttcttcggc acgatctgga agaggaaggt gccgcacatc tacgtcgcca
      181 actggttctt cggcgccttc atcctgacgg tggcgctgct gcacatcgtg aacagcgccg
      241 cgatgccggc gaccttctgg aagtcctact cggcctacgc gggcgcgacc gacgccatgg
      301 tgcagtggtg gtacgggcac aacgcggtgg gcttcttcct caccgccggg ttcctcggga
      361 tgatgtacta cttcatcccc aagcaggccg ggcgcccggt gtactcgtac cgcctgtcgg
      421 tggtgcactt ctgggcgctg atcttcacct acatgtgggc gggcccgcac cacctgcact
      481 acacggcgct gcccgaatgg gcgcagtcgc tcggcatggt gttctcgctg atcctcctcg
      541 cgccttcctg gggcgggatg atcaacggca tcatgacgct gtccggcgcg tggcacaagc
      601 tgcgcaccga tccgatcctc aagttcctca tcgtctcgct ctccttctac ggcatgagca
      661 ccttcgaggg gccgatgatg tccatcaaga cggtgaactc gctctcgcac tacaccgact
      721 ggaccatcgg ccacgtgcac agcggcgcgc tcggctgggt ggggctggtg tcgatgggct
      781 cgatctacta cctgattccg cgcctgttcg gccgctcgga gatgtacagc gtgccgctca
      841 tcaacgtgca cttctgggtg tcgacggtcg ggatcgtgct ctacatctcg gcgatgtgga
      901 tcgcgggcgt catgcagggc ctgatgtggc gcgcggtgag cgaggacggc acgctgacct
      961 atacgttcgt ggagagcgtg aaggccacct accccttcta cgccatccgg ctgctgggcg
     1021 ggctgctcta cctcggcggc atgctgatca tggcctggaa cgtggcgaag acgatcggcg
     1081 cgggcaggtc ggtggaggcg ccgatcccgg cgcccgccta cgcctaggga gcgcgcgatg
     1141 aaactcaccc acgacatgat cgagcgcaac caggcgctcc tcatcatcct cgtcatcctg
     1201 gtggtcgcgg tgggcggcct ggtggagatc gtgccgctct acttccagcg ctcggtcacc
     1261 gagccggtgg cgggcctgaa gccctacggt gctctgcagc tcaccggccg cgacgtctac
     1321 gtgcgcgagg gctgctacaa ctgccactcg cagatggtgc ggccgttccg cgccgaagtg
     1381 gagcgctacg ggcgctactc ggtggcgggc gagttcgtct acgaccggcc gttccagtgg
     1441 ggctccaagc gcaccggccc ggacctgcac cgcgtcggcg gccgctattc cgacgagtgg
     1501 caccggctgc acctcatgaa cccgcgcgac ctcgtgcccg agtcgaacat gccggcctat
     1561 ccgtggctgg cgctcactgc cgcgaacgcc ggggacatcg gcgccaagat gcgcgccctg
     1621 cgcacgcttg gcgtgccgta cgccgacgac gagatcgcca aggcgccgga ggagctgaag
     1681 ggcaagaccg agatggaggc gctgatcgcc tacctgcagg tgctcggcac cgccgggcgc
     1741 gcgggcgccg ggaaatagga ggccgcatgg acctcaacct gattcgcagc ctggtcaccg
     1801 tggcggcgct cgccgcgttc ctgggcatcg tctggtgggc gtactcgccc tcgcgcaaga
     1861 agcgcctgga agacgccggg cgcagcgttc tcgaggaaaa cgaccaatga gcgagttcac
     1921 cagcagcttc tgggactact acatctccat catcaccctg gtgtcgatcg tcgcctgcgg
     1981 cgtgctgctg gtcgcacaga gcaagcgccg cgtcgccggc ggcaccgccg agaccaccgg
     2041 ccacacctgg gacgaagacc tgggcgagta caacaacccc ctgccgcgct ggtggatgtg
     2101 gctgttctgg atcaccatcg tcttcggctt cgcctacctg gcgctctacc cgggcctggg
     2161 cagctatccg gggatgctga agtggacgca ggtgggccag ctcgagcggg agaccgcgcg
     2221 gcacgaggag cgcttcggcc cgctgtacga gcgctacgcg aagaccgacg tggcggcgct
     2281 cgccaaggac cccgccgcgc tcgccatcgg ccagaagctg ttcctcaaca actgcgcgca
     2341 gtgccacgcg tccgacggcg ccggctcgcg cggctttccc aacctgaccg accgcgactg
     2401 gcagtggggc ggctcggcgg aggccgtgaa gaccaccatc acgcagggcc ggatgggcgt
     2461 catgccgccc ttcgggccgg cgctcggcga gcagggcgcg aaggacgcgg cgcattacgt
     2521 gatggcgctc tccggcctcg cgcacgacag catccgcgcg gcgcgcggca aggcgctgtt
     2581 cgcgcagaac tgcgtcgcct gccacggcgc cgagggcaag ggcaacccgg cgctcggcgc
     2641 gccgaacctc accgacagga cgtggctgta tagtgcggcg gagcccgtga tcatcgagac
     2701 catcacccgc ggccgcacga accagatgcc cgcccaccag gacctgctcg gcgaggcgcg
     2761 catccacctg ctgaccgcgt atgtgctgtc gctgtcgcag tcggcgaagt gaaagcgacg
     2821 tccgtccccg tccaggcggt cgagcaggcg ctctacgaag tccgcaagac catccacccg
     2881 cgcgcggtgc acgggtggtt cgccggctgg cgctgggcgc tggtgtgggc cacgcagctc
     2941 gtgttctacg gcctcgcctg gctgccgtgg aacgagcgcc aggcggtgct gttcgacatc
     3001 gcgcaccgca agttctacat cttcggcctg gtgttcttcc cgcaggacat catctacctc
     3061 accgtcctgc tgatcatctc ggcgctgtcg ctgtttctgt tcacggcggt cgccgggcgg
     3121 ctgtggtgcg gctacgcctg cccgcagacc gtgtacaccg agatcttcct gtgggtcgag
     3181 gagaagatcg agggcgaccg tccggcgcgc ctcaagctcg acgccgcgcc gctctcgccg
     3241 cgcaagttcg cgctcaaggc cgccaagcac ggtgcgtgga tcgccatcgc gctgtggacc
     3301 ggcttcacct tcgtcggcta cttcacgccg atccgcgagc tgtgggacgc ggtgtggtcc
     3361 ttctcgacgg ggccgtggga gacgttctgg gtgctgttct acggcttcgc cacctacggc
     3421 aacgccggct ggatgcgcga gcaggtgtgc aagtacatgt gcccctacgc gcgcttccag
     3481 agcgcgatgt tcgacaagga cacgctgatc atcacctacg acccggagcg cggcgagccc
     3541 cgcggcaagg gccccggcaa gggccattgc gtggactgca gcatctgcgt gcaggtctgc
     3601 cccacgggca tcgacatccg cgacggcctg cagtacgagt gcatcggctg cgccgcctgc
     3661 atcgacggct gcaatcgggt catggacaag gtcggctttc cccaggggct gatccgctac
     3721 tccacgcagc acgcgatgga ggagaagctg acgcgcgcgc agatgttccg gcgcgccttc
     3781 cgcccgcgcg tgctggtcta cagcgccatc ctgtgggcgg tcatcatcgg cgcggccgtc
     3841 ttcctgtgga accgcgtgcc gctcaaggtg gacgtgatcc gcgaccgcgc ggcgatctcg
     3901 cgcgaggtcg agggcgggca gatcgagaac gtgtaccggc tgcagatcat gaacacggcg
     3961 gagggcgcgc gcgccttccg cctttccgtg tccgggctgc cgggcatccg cattcccggc
     4021 gaggccgtgg tcgagatcga cggcgccgcg agccgcatgg tcccggtcaa ggtgcagatc
     4081 gcgccggggg cggcgggcgc gggcacgcac cgcatcgagt tcgcggtaca gtccacggac
     4141 gaggcgagcg tcgccgtgcg cgagaagtcc gtgttcatcg tgaggtgagg ccatgacacc
     4201 ctggtacaga gagcgctgga cctggatcgt gatggcgccg ccggcagcgg cggtgctcgc
     4261 gggcctggcg acctggtgga tcgccgcgag cggcgccgac ggcctggtgg ccgaggacta
     4321 ctacaagcag ggcctggcca tcaacaaggt gatcgcgcgc gaggagcgcg cgcgcgcgct
     4381 cggcatctcg gcgcgtctgg acatcgcgga cggccgaatt cgcgtgcgcc tggagggcgc
     4441 ggcgcccgag gcgctgttcg tgcacctcgc gcaccgcacg cgcgccggc
//
